LOCUS       DAOPWB010000001        80703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_253_length_80703_cov_6.226044, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000001 DAOPWB010000000
VERSION     DAOPWB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 80703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 80703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80703
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_253_length_80703_cov_6.226044"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="PKN92_00005"
     CDS             <1..124
                     /locus_tag="PKN92_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNZ47200.1"
                     /translation="PLVGGGGGGRADMAQAGGKDIEKLPEALKKAETIIAELIK"
     gene            265..1377
                     /locus_tag="PKN92_00010"
     CDS             265..1377
                     /locus_tag="PKN92_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47201.1"
                     /translation="MPYLQFDRDKLFVKSLSQRKDKVFIERDHVPVNASVAKPLSRLG
                     QKTMNELADRLILAKKTNKSRMLTFGAHAIKNGLAPVFIHLMKEGWVTHLATNGAGII
                     HDWEFAFQGHSSEDVRANVDRGEFGIWQETGTYLNFAIVAGAWKGLGYGESVGAFVEN
                     DGVEVPSQDELVEEIREQAAQNPAQAAAAADMLDRVRHFDLKPGFMSVPHPYKKFGVQ
                     AAAYRLGVPFTSHPMIGHDIIYTHPMNYGAAIGRAAQRDFLVFAQQVMNLDGGVYMSV
                     GSAVMSPMIFEKSLSMSQNLHIQEGKHIDNHYMVIVDLQESHWDWSQGEPPMDNPDYY
                     LRYNKSFNRMGGQMRYVTADNRDFLLTLVKTLEERK"
     gene            1381..3678
                     /locus_tag="PKN92_00015"
     CDS             1381..3678
                     /locus_tag="PKN92_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47202.1"
                     /translation="MMIAFLMLFIAMAGSTAKACAAFQVPEVRISPEHPLILLQSSAA
                     FGDADSSQEQAKRVEEARRHGEEIVRVWNLLPSDIRPLCQIQIELRVQEHSLRKMLFE
                     EMLRPVQANEVAVSLQIADPHDEYVFDLNAVESLLQTFPCIKSLMVSEQQFAHYAKFN
                     VEDYAVPPHVRYCMDVIQLGARYGKHTVLVLQGLKWLHIGADTLNRPLLEAMRSFSDY
                     VIPVNEHIEPRHLIRQTAVWGLWLGDFVTHWGVEPQSWWFESSFMNTPGIFGDHLHPA
                     EMPPEMYRPMILQGAAMGATVYSFEPWWDLFDYGNSRCWDEVILPTLREVIQSELIPS
                     KEQVLEKTPVAYQLAPARSIHDFHINMRDLDWLSDDGTLAQLVYGVWEPMLEFELIPN
                     KSNWFIPLLPAVVSEEAAGRFPRLLHAGDCDDEACWREQLSGYLKEPASIPQAWTCEI
                     NDHAYVMHTHENLYEKQFFEVETAQPVRNITASLTENGSLQLNWDEVPQTASYEVCFT
                     SAKGSTAVLATVSETSYIVNLPEPGKFSVTCTTRSRERYSGSVNYLDCLVFSQRTSRP
                     VEHILFTKEGTVLNEKEEAVTDTRPESQQIYPDYSGVPDQFQRLAEEVTGALDEFKNA
                     YESMDWKRLTALYDPAYEDANGFHREYVSRAWKWWFRRNNKCFLLRQIRNWDFSEYSH
                     SEIVKNMLFLYCTAVRWDDQPFGYDGIVRIPRHKGAEVQCSWIKKEGRWRLLKTEPSL
                     PNLEEILWNSRPMDKEEKLVPSLDE"
     gene            complement(3808..5727)
                     /locus_tag="PKN92_00020"
     CDS             complement(3808..5727)
                     /locus_tag="PKN92_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNZ47203.1"
                     /translation="MLSSFFLSLLIAAHPTDTHSLTPLALEPFPLGKIISDGWLEEQM
                     RIQSNGLSGNLDKFWPDIQHSGWIGGNAEGWERAPYWLDGLIPLAWTLPDVHLQKTVH
                     FWMRYIIAHQEKDGWLGPKKSEGYQEHDTWPQFVILKALRQYYEVTGDSSILEVMTAN
                     VRCIDRILIYKPLFDWGKYRWMDFVITLHWLFDRTGNPDFLNIAERAHSQGFSWRDHF
                     ENFRFTEKMKGSECIFDTHVVNNAMAVKAPAVWYRQSRDPGDLHAVQLLLDTLDKYHG
                     QVTGIFTGDEHYAGKNPSQGTELCAVVEYLFSLETLLAYLGRAEDADRMERIAFNALP
                     GTFSPDMWAHQYVQQANQVICRVSDDRIYTNNGPDSNLFGLEPNYGCCTSNMHQGWPK
                     FTSHLLMRTPAANDKPQGIAAVAYAPCSLKTTLNGQPVFLRIETEYPFEETIRIHLDA
                     TGEFPVHLRIPAWAEGATVQLNNEAPVSAVPGTFHVLQQKWTCNSVILLRFPMKAVRM
                     ERFNKSAAISRGPLVYSLLVETEWRSLRGEAPHNDFELYPVSPWNYALAPDFMETIRF
                     ESAPLSCNPFDPASTPVRAYVKGRLLPEWKIEHNAAAPPPLSPVHSEEALSELTLIPY
                     GAAKLRVTEFPVSDK"
     gene            5908..7167
                     /locus_tag="PKN92_00025"
     CDS             5908..7167
                     /locus_tag="PKN92_00025"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNZ47204.1"
                     /translation="MKSRCFIFSLLFVLVQTGSFAQTEGSERYRLLSAREYEERMTAA
                     WIGQMVGVGWGVPTEFKFNGVMVPEKEFPRWTPETVNQFQEDDLYVEMTFLHTLEKYG
                     IDATPRQAGIDFANSGYELWHANKFGRQNLRKGIAPPDSGHPEYNSHADDIDYQIEAD
                     YSGIIAPGMPSIVLKLGDTFGRMMNYGDGLYGGLFIGAMYSEAFFESSIEAIIQAGLK
                     AIPEQSQYHECISDVLRWHKENPEDWTKSWEKINEKYHLNPAYRKFSCGDRGAFNIDA
                     KINGAYVVMGLLYGEGNPDKTIEIATRCGQDSDCNPSSAAGILFTTLGINNIPEKFTS
                     ALKRDVAYSHTPYTFDGLLAICNELTRQAVVYSGGKILHDAEGTEYFCIPVQEVAAGA
                     LEQCWEPGPVANSRFTEEEYAQILTKE"
     gene            complement(7181..7789)
                     /locus_tag="PKN92_00030"
     CDS             complement(7181..7789)
                     /locus_tag="PKN92_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNZ47205.1"
                     /translation="MTEKLSTQEELELLVQKIRRCTVCAAHLPLGPRPVVHLHSEARI
                     LIVGQAPGRRVHETGISWDDPSGDRLRDWMGIDRDVFYNVRKIAIMSAGFCYPGRHPR
                     GGDLPPRPECMPLWHPQVLPLLPKIELTILAGTYAQKGYLKNRRKSSLTDTVKHWKSY
                     IPRFLPLPHPSARNIAWFQSNPWFEEEVVPYLRDRVHTILSL"
     gene            complement(8003..8836)
                     /locus_tag="PKN92_00035"
     CDS             complement(8003..8836)
                     /locus_tag="PKN92_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ47206.1"
                     /translation="MLEPSDRSLKRYLQEISKIPLLPASEEIRLAKLAKEGDSSARRK
                     LIVSNLRLVISVAKKYLYFGLPLLDLIEEGNLGLMRAVDRFDPSHGCKFSTYATWWIR
                     QAVTRALSNQGRTVRIPVYITDNLAKYKRVAEEFYIQNGSYPSSDDMAAALGMTLKEI
                     KKLELFDQTVSPFDNMQSLDSAIGRDTCTIQEPAAANQALVQFFRDEEMKTLMAQLNE
                     RERSIISYRYGLIDGKAHTLEETGKHFELTRERIRQIEREAMRRLRKYMSIHGDDFTK
                     S"
     gene            complement(8870..9688)
                     /locus_tag="PKN92_00040"
     CDS             complement(8870..9688)
                     /locus_tag="PKN92_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47207.1"
                     /translation="MGVPFVLIQDIRLSYQEAIQLIRIGHYDAARNLLKRINEAQPNV
                     KNVLYPLAVCCEYLDLVDEGLDYCDQLISLFDHDKAKMIQARLLEVLPVPEEVQEIPK
                     TEASVSPPPQKDSDETFVAPVGTVDTNKAPQGASDTLDESSPPFSDNPEVFETQAVAV
                     SLPDAKAEEGNFIDTCRVPPDVEEKDIAAERESSELMYEEATAVLEEIMDEESARDNP
                     SVHIPLWFVLLLAFLAAVFSAALIHYFILGTEGPVLLLNELFAKMMAAYHSLMQ"
     gene            complement(9741..10883)
                     /gene="thiH"
                     /locus_tag="PKN92_00045"
     CDS             complement(9741..10883)
                     /gene="thiH"
                     /locus_tag="PKN92_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HNZ47208.1"
                     /translation="MTFEAYLNEWPETLIQEQYTTLSERQVEAALSADRRTPENLLAL
                     LSPAALPFLEAMAQEANKQTRRQFGRTVSLYAPIYLSNVCGSNCLYCSFSTVNAGKET
                     RTTLTPTQIEDECRTLAKEGFSSVLLLTGDAVHAAPPEYIAQACTIARRWFSSVAVEV
                     YSMTQEEYHLLCTHGLDGVTLYQETYHKPTYLDMHRSGRKRDFSFRLEALERAANAGV
                     RRLGAGVLLGLYKWRVDAFWLALHARWLQTRCWNSTIALSFPRLKHTPSAFNPPFPVS
                     DINMVQMILALRLFLPESPFYLSTREAPSFRDKLLPLGITAMSAGSSTRPGGYAAPTS
                     PTLEQFQIEDVRTVAEIKTFIEKSGYDPVWKDYDQAFTEAVPKQLQ"
     gene            complement(10892..11671)
                     /locus_tag="PKN92_00050"
     CDS             complement(10892..11671)
                     /locus_tag="PKN92_00050"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNZ47209.1"
                     /translation="MDTFTIAGRLFSSRLMIGTGKFPSAAALRAAIEASGSEIVTVAL
                     RRVDLNSPEHQSILSAIDPKKQLILPNTSGAQTAEEALKLARLARAGGLEPWIKLELT
                     PEPRYLLPDPIQTLRAAEQLVKEGFVVLPYIQADPVLAKRLEEVGTATVMPLGAPIGS
                     NRGVQTKEMIRIIIEQAHVPVVVDAGLGAPSHAAEAMELGADAVLVNTALSDARDHGA
                     MAKAFALATTAGRMAYLAGLGPQRSLAAASSPLTGFLFNQD"
     gene            complement(11774..11956)
                     /locus_tag="PKN92_00055"
     CDS             complement(11774..11956)
                     /locus_tag="PKN92_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47210.1"
                     /translation="MKKAPLSVLSLALRPVLPEMSFFCREQIQSPVFILVLYDLKQAR
                     SDSFQQRTALFGSAEY"
     gene            11955..12950
                     /locus_tag="PKN92_00060"
     CDS             11955..12950
                     /locus_tag="PKN92_00060"
                     /EC_number="3.1.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF027980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ca2+-dependent phosphoinositide-specific
                     phospholipase C"
                     /protein_id="HNZ47211.1"
                     /translation="MSSILLCLAVLLSLNQRDDFHAPRMNQIQVIGTHNSYHLEPPSL
                     VLDTLSTLDPRVKEWAYSHDSLDAQLEQGVRSFELDIHPYVSGFKVRHVPLVDDNSTC
                     PEFMECLSTLYLWSLEHNEHVPVTILVEIKQAEALLAAEPLCNDPVQIVQRIEDEIRT
                     IFPSDKLVTPSWVQGNAVSLRKKLELEGWPPLKHCLGKFAFILHDRGNLRDACASAGQ
                     NKVLFVNASPARSDGAFIVVDDPYNPEISALLKQNMIVRVRADSGLNVDRPDSIKRKE
                     QALACGAQIVSTDFPPGKADPATGYCLSLGESTSARSNPVTGDFSVKAFLQTIIP"
     gene            13127..14491
                     /locus_tag="PKN92_00065"
     CDS             13127..14491
                     /locus_tag="PKN92_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNZ47212.1"
                     /translation="MRRYVYNLDPQKMPDAWYNILPDLPTPHAPVLHPATHKPVVPED
                     LAPIFPMNLIEQEMSSEPAISIPGEVMDRYLMFRPTPLRRAYRLEEALQTPAKIYYKN
                     ESVTPSGSHKINTSIPQAYYNKVEGVQSLSTETGAGQWGTALSIACAMFNMQCKVYMV
                     RVSFDQKPYRKGFMETFGATVIASPSDTTEVGRKIRAANPGTSGSLGMAISEAVEVAV
                     TSGGTVKYSLGSVLNHVCMHQTIIGEEALLQMEMAGDYPDVVIGCCGGGSNFAGIAFP
                     FVREKFNKKLDTRFIAAEPTACPTLTKGPFAYDFGDTGQLTPLIKQYTLGHNFVPPGI
                     HAGGLRYHGVAATVAHLKKDEVIEAVALHQNPCFQASLMFAKAEGIIPAPESGHAVRS
                     AIDEAIKCRESGEAKTILFNLSGHGHFDITAYEAYLRGNLQDLELDEETLAKGIASIP
                     EIQE"
     gene            complement(14551..15438)
                     /gene="rfbD"
                     /locus_tag="PKN92_00070"
     CDS             complement(14551..15438)
                     /gene="rfbD"
                     /locus_tag="PKN92_00070"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNZ47213.1"
                     /translation="MSRTLIIGSAGQLGSELLCAFPPGEAFGADLEAADFQLDITNYN
                     ALRSCITEVQPSCVILTAALHEPAFCEAHPQLAYQINALSCLETAVLCRHVGARLIYI
                     STDYVYGGYSFSPARPFQEIDPVRPVNVYGKSKTAGERYVIENCSDYLIIRTASLYGQ
                     NPCRGKKGRNFVETMLARAREGSPVHVVTDEMCSPTCARDLAEQISILATAAPSGIYH
                     AVSKGFCSWYDFAREIFFQFKIDADLLPVQRMNFQDAFRRPAYSLLDRSKLARSGLDY
                     MPDWQDGLKKYAAARSLEI"
     gene            complement(15492..16040)
                     /locus_tag="PKN92_00075"
     CDS             complement(15492..16040)
                     /locus_tag="PKN92_00075"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HNZ47214.1"
                     /translation="MKINLLVSLLLLASLFFTTSLCAAEGAHPGKFNIELSGGPGLEL
                     GGTDRSGDFLLKSTLEFEKPFSSHFSLGLRLLPLFLYEQKSKGDDTVWGGGLGLGLRF
                     YFERDSYRGVYLEANTHALAHKGRIEGNGSNLNFLSAFGVGYGFQNGLHTVLRYEHLS
                     NGGLDSHNRGVDVLTLGLGFTF"
     gene            complement(16125..17330)
                     /locus_tag="PKN92_00080"
     CDS             complement(16125..17330)
                     /locus_tag="PKN92_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ47215.1"
                     /translation="MLEPDANKPVAKRPKRFDLGSSESEQTGASLTGSSTDAQTAPAR
                     RLFHGSGNVRYADVTNFLRQFIMLLESGTPILRSLRSLASRAENLAVKGLINDIALYV
                     EGGNTLWQAFDRHPRYFDSVFVNLIKASEASGTLTTVLRRLVTYREGREMLRKKVRGA
                     MIYPVLLIIACAGALFVITSFVVPVFADFFERADMNTPASTKLLIELSALTRLWWWLP
                     FVLLVILGLVYKLWLTKTPERRIKADYYKLKIPVLGAIIHKNALVEMCRTMGLLLRSG
                     LSMMATLDLTRSAIHNRAVAETLKAMRHSVEQGGSLEEPMRQSKSIPPVVTDMFVTGE
                     ESGKVDQIAEQIADIYDEEVKLAVAGLGEVLQPIFTIIVGIAVLILFVSLFLPMITMI
                     DQLGAGSSL"
     gene            complement(17380..17973)
                     /locus_tag="PKN92_00085"
     CDS             complement(17380..17973)
                     /locus_tag="PKN92_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47216.1"
                     /translation="MSNKRTIKRAVTPLFFCCPERCDGRRGAALFTAIALLTLFTLLG
                     ASYVHFLMVEDDLCTFDLRKLRSQFYAEAGIQSSAAYLQETLRKGMLPVTSTIYTFPV
                     YGYTQGSNPDRPVLLDNYRAEVSASMSALSAEDWQEFNFSPENFPGEGLVWKIESSAV
                     LLKNAALDAYPLSRHSIAAVVSLDRNEIQVLSWRTCR"
     gene            complement(17963..18598)
                     /locus_tag="PKN92_00090"
     CDS             complement(17963..18598)
                     /locus_tag="PKN92_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNZ47217.1"
                     /translation="MNITYYSRPNRGFTLLELLLVMSMLGVVSTMGFVGFVKVSYQWN
                     DLVSALTLEKTLARCFTEFRKDFENILPTDCCQRGLEGMRASVEDSRRYWNVNFEDDS
                     VSFPVSIYNPLQDRNDRFLVRYKIERTTESARFIRYVSELDIEDAVPQESFSLENILA
                     MRILYSDGKRWNTQWNRREMPDAVRISLTCVDPRDPTRHISRMVVFNIHVK"
     gene            complement(18595..19146)
                     /locus_tag="PKN92_00095"
     CDS             complement(18595..19146)
                     /locus_tag="PKN92_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNZ47218.1"
                     /translation="MVCRKSLFYNNKAAAGFTLLELITALAVISVALAISVSLFGNNL
                     QRSKELRNNRVAAEIAETQLSVLCAHPGEYVWSFDETGEDGLFPILPNENSTLAASGA
                     LLPDTILATKKAQDKTATLHRSFYWQAWGRIPSETADYLEVTLSVHWRLSGRPRMLAL
                     TSCVPRSLFQSLDQAKETEAGPQ"
     gene            19379..19516
                     /locus_tag="PKN92_00100"
     CDS             19379..19516
                     /locus_tag="PKN92_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47219.1"
                     /translation="MHCALCNEFVEDNELACGDAIEVDGEYWHSECYVEYYGEELEEA
                     V"
     gene            19706..19990
                     /locus_tag="PKN92_00105"
     CDS             19706..19990
                     /locus_tag="PKN92_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNZ47220.1"
                     /translation="MNAVLLKIRAYPRASRNRVIVTESGLLQVYVTAAPEDNKANTAI
                     IATLSKLLKVAKSNIEIVSGLQGRDKKVKVSNVSEKIINQRLELLQADKS"
     gene            20041..20850
                     /locus_tag="PKN92_00110"
     CDS             20041..20850
                     /locus_tag="PKN92_00110"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNZ47221.1"
                     /translation="MKKIEQAAEVVQKHLTWQPEVAIILGTGLGSTGSSIENPVVIDY
                     AAIPNFPLSTAEGHAGEIIAGTLAGKKVIGLSGRFHLYEGYSMEEVTFPVRVVKALGA
                     HTLIVSNASGGLNPQYRAGDLVVITDHINLMGNNPLIGPNDDRLGERIPDMSEAYSRA
                     LVSLAEQAALEEQIKLQRGVYLGCTGPCLETPAEYRFMRIIGADLVGMSTVPEVIVAV
                     HAGLKVLGFSVVTDECFPDCLQPLNLERIVRCAAEAEPKLTKLINRCLHLL"
     gene            20888..21934
                     /locus_tag="PKN92_00115"
     CDS             20888..21934
                     /locus_tag="PKN92_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNZ47222.1"
                     /translation="MLVKISRVFFLLACILMGFIWSKFILENMVESGRQIENVQRWIG
                     LGGLIGAVVAIAVLLALHFVTQELYERFAPAVVAIVLALLAGYSISRYLLFWFPSIDI
                     TSQVFISVTVVLLFGYVGIYLGLTRASNWESLISAVKHRQFQNSPTVSLKIVDTSVLI
                     DGRIADICESGFLEGTLLIPRFVLKELQNIADSADVVRRAKGRRGLDILKSLQESTAE
                     VSVSIIEDNPNASKEVDGKLLLLAKQYGAKVLTTDFNLNKVAQIDGVVVLNINDLANA
                     LKPAVLPDEIMEVKLIKEGKEPSQAVGYLDDGTMVVVDGGRNHLGKNVQVLVTSILQT
                     SAGRMIFTRFHTQA"
     gene            21934..22611
                     /gene="ispD"
                     /locus_tag="PKN92_00120"
     CDS             21934..22611
                     /gene="ispD"
                     /locus_tag="PKN92_00120"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNZ47223.1"
                     /translation="MRVQAVIPAAGMGERLGASRPKALVEINGVPLLRHTLLRLASLL
                     TPLPCIVSYPEGFRNEFEHACCDLNVVLICGGRERQESVWLALNVLDPQVEIVLLHDA
                     ARPFPDPASIQCAVEETKKWGAATLAIPVSDTILSVSEDGFLKDTPDRRELRACQTPQ
                     IFLKETITAAHRAAHEEGRQFTDDASMVRFYGGQVKIVPGTVGNFKITTPADLAYATY
                     LVQEGKE"
     gene            22608..23099
                     /gene="ispF"
                     /locus_tag="PKN92_00125"
     CDS             22608..23099
                     /gene="ispF"
                     /locus_tag="PKN92_00125"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNZ47224.1"
                     /translation="MIRIGIGYDIHRLVPGRKLILGGVCIPHEKGFEAHSDGDVLCHA
                     ISDALLGAAGLPNIGVLFPDTDPAYKDADSISLLKNVVRRLHDRGFRPVQMDSNIVAQ
                     RPKLKDYVEEMIHSLSAATGMPSENISIKPRTNEHLGAEGREEGISAQVVVVIEKIPP
                     EEA"
     gene            complement(23102..23722)
                     /gene="purN"
                     /locus_tag="PKN92_00130"
     CDS             complement(23102..23722)
                     /gene="purN"
                     /locus_tag="PKN92_00130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNZ47225.1"
                     /translation="MTIRLAVLLSGSGSTLQNLIDRITGKTLDAQISCVIASRKDAYG
                     LVRADKAAIPSYAVPRTDYSTQKDFNAAIWEHLGRHEVDLIVLAGFMHLLEVPPAFAN
                     RIMNVHPALIPAFCGHKMYGNHVHKAVLAYGAKITGATVHFVDSLYDHGPIILQDSVP
                     VTPEDTVESLADRVQALERELYPKAIALYAAGRLSVDGRVVHILPE"
     gene            23856..24677
                     /locus_tag="PKN92_00135"
     CDS             23856..24677
                     /locus_tag="PKN92_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNZ47226.1"
                     /translation="MKRFLFFVMIVTALTLSGCETLEGTAGSGFFNQINLLSTQQEVS
                     LGQQFSVQIEESEKVLNHPAIQNYVSEIGKKLIAVAPRQDVPYSFKVIDAPDTVNAFA
                     LPGGYMYIYTGLLKTCSNEAELAAVMAHEIGHVAARHHGEMMTRQMGMEMVTDMVLGK
                     KPGMVASLAGQLFTAGVSARYSRAQEREADSLGMEILYRAGYPPQGMISFMSKLMEAD
                     RRQGGGRSMPIFATHPSPEERVALLQELLQRYPATPQHNANYGEDRYRQAILSQL"
     gene            complement(24684..26183)
                     /locus_tag="PKN92_00140"
     CDS             complement(24684..26183)
                     /locus_tag="PKN92_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ47227.1"
                     /translation="MTDRQKLNVLWICSDEFRPECLGAAGHPFVQTPNLDALAAEGVY
                     FRNAFCQSSPCAPSRMSMHTGRYACSTGVVDNLTPLKNAEHNLAVHLQRFGLSPAIVG
                     YNDYSVDPSLLPEGHPHKHSLSYEHFLPGYEVVLKHEYDSPEWYDWLKTQGYPEQHCT
                     HETMYSPCIPETGAGAHLPCFYPARYKREHSEMAFVTNKSMEYIAGNADKQWFLSLNY
                     IKPHGPYLCSAPFHSMYDPKEMPAPVRHPREAKNDHPYFSRCRADWAQTEFLDEGSWR
                     DVRACYYGMISELDYNLGRLFDFLKESGQWEHTAIIFNSDHGTYLGDHYLAGKAHYFD
                     AAVRVPLLIRDPAAEADSTRGTLNDVLIENVDIAPLICNFLGAPPSSGFQGKDPRYLL
                     TDPNCRNHKSTIIFEFYYYNILKETANLNPEACRLWMLRDHKYKYVQFGEASMPPQLF
                     DLQNDPGEFENLAQQDACSGIVADCCQRLMRWRMGCEQNALEAWARQYR"
     gene            complement(26228..28432)
                     /locus_tag="PKN92_00145"
     CDS             complement(26228..28432)
                     /locus_tag="PKN92_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HNZ47228.1"
                     /translation="MTDSIHADSSSVESTHAGQEMSAHPGGAISVEGVIDKIVYENVE
                     SGFLVGRLLTGDGSALTFIGNLAAVSAGETVRLRGHWVDNLKFGRQFKLSSYEIIQPT
                     SREGIQRYLGSGLIDGIGPVYAKRIVDHFGLQSLKIIADSPERLKEVPGIGKKRAGQI
                     KKAWAAQNAARSVMLFLQSYNIPQGLSIRIYKQYGESAAAQLRANPYKLSTELTGVGF
                     LTADKIAHQMGIAKDSAFRIEAGFLFVLEEQSEKGHVFFPIDQLRECTGELLKLSADT
                     LIEPFSGLIEKRKIVCDNDACYLPLLYAAEKGCAARLDKIAGAPLEAIKIETDKAFQW
                     VENRFRISLAAEQRRAVEKAISSKVMVITGGPGTGKTTIIKSVLEILNAKGLSSLLVA
                     PTGRAAKRMEQATGQEARTIHRLLEFSPLTGSFTRNEHNPIETDLLVVDEASMLDIHL
                     AFALFNALPLHCRLVLVGDSDQLPSVGPGSVLRDIIDSGRVPVVRLNTIFRQKEESAI
                     VTNAHRINHGETPLFNQHDFFMIEREKPEEAVSTIVDIVTRRLPSSFHLHPLKDIQIM
                     TPMRRGSAGVDAINAALRAALISQQNTVDLAGFSVGDKVMQMHNNYDLDVYNGDVGLV
                     TQVDQEAKELEVCFEDERRVIYSTDQAHQLASAYCTTVHKSQGSEYPAVVLCLLPQHY
                     MMLQRNVLYTAITRGRQKVILVGSSKAVSLAVNNNTITRRYTRLAERLQHII"
     gene            complement(28429..29148)
                     /locus_tag="PKN92_00150"
     CDS             complement(28429..29148)
                     /locus_tag="PKN92_00150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ47229.1"
                     /translation="MTQRKTALVTGAGRGIGRAVALALAGIGYEIALTARTESELADA
                     AAAIRSKGGIAEVFPCDLTEQGAIKQLANAVQEKFSPLDLLVNNAGSAVFKPVWELSA
                     EDWLHSLTINLTAAFLLTRAFLPQMMTRRQGRIINISSVTGLKAIDGQAAYCAAKHGL
                     NGFTKSLALELRPYNIAVHAVCPGGVDTQMSRDAMPHRDKTGWMTPEDVAQTVLYLVS
                     LSPRTAIDFVTMRRFDSEPLA"
     gene            complement(29145..30749)
                     /locus_tag="PKN92_00155"
     CDS             complement(29145..30749)
                     /locus_tag="PKN92_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNZ47230.1"
                     /translation="MLSQLLETLHKISAAFQRGRQFITHDIWRIGRPGEKVPSGLIIK
                     QIRAAILLVRGLMEETLLLRASALTFATLLFLVPFLALMFYFIQTFNLGDQIYEKMDE
                     RLSKAVVALRHIGDTEEEGEEESKRQEELDELFPVADENADDNTAVLGVHPENFSGSD
                     EQLKRHLITTLFPILAEKDGITNDPLYQNPVKYLVSFADEAARSPRALGVSGLLFILT
                     TVFGFMRNVEYTFNRIWGVSRQRNPLRVIGHYMLLTFMLPFVAAFIMGISAALENSYV
                     LEILGPAALLLRSAQFLVLSLSFTLVYFFVPNTKVQFRYALIGGLVAASLYMLNSWIY
                     IKFQYGLINYTNFFFAFALFPVLLMYIYFSWLLLLFGALVSFAYQNEKTFALERLAAE
                     TTTAYKESLAVRTMLELSFRFKNSLEPLQIKEAAELWNVPTRLLQETLDILKDNRMVI
                     ATDSIPSSFHLARSAHSISVLEVVNAIRNTGRDPSLLREEADYKVVYEGLDTADPQVL
                     TQSIQSLSDQILDKKDFSAGGSSAIP"
     gene            complement(30809..31483)
                     /locus_tag="PKN92_00160"
     CDS             complement(30809..31483)
                     /locus_tag="PKN92_00160"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ47231.1"
                     /translation="MVLPVLAFCILAFSSTAEETGFVSIFDGASLDGWEGKDGEWRVE
                     DGAITAESTPEHPCPAAHYLYWKGGEPQDFILRFAYKIQGEGGNSGVHFRSEARPAWD
                     IWGYQADIEKGKEWTGCLFQHDRGGVVMRGFKSYIDEEGRKTETVLDDPAKLLEAVHQ
                     DVWNTYEICARGASITLSINGQIMCSVEDHDKVFSRQKGHIALQMHPGPPMKIQFKDL
                     RIKILE"
     gene            complement(31528..32892)
                     /locus_tag="PKN92_00165"
     CDS             complement(31528..32892)
                     /locus_tag="PKN92_00165"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47232.1"
                     /translation="MISRRNFLKTTAVAPLVLSSRVRGQSTPGPNSQINVAVVGLGGR
                     AQEIVATCLGIPELRVAAVCDCFAPRLNSSLKALADKDITCAGYLDYQEMLEKERLDG
                     IMAITTTHARAWVSCLAMAAGLDVYIEKPMCLTIAEGREMVKLARHYKCVTQVGTQQR
                     SMPLNNWASDLVKNGAIGKLKAAHVPDFISPLTWQPKPAESLPDGGRADWWDVWLNQT
                     PLLPYHSELHRGWAQWDAYDGGGISFGVTGWGTHSYDQLQRGLGTDLTGPVEITLEEE
                     VQDRPACPDKTRQPGENETGAPYYGMVLNTTGPRARVSMRFANGTPVYFTFDGNNGPG
                     LGAVFEGEDGIIEINRDRIYSTRQDLVADPSNPGPLSVPETQPHIENWLQCMKTREKC
                     NADVEIGQRSTTLCYLINIVRKIGLVNKPLHWDPETEKFTDCDEANAFLARERRPAYE
                     LPTI"
     gene            complement(33000..34310)
                     /locus_tag="PKN92_00170"
     CDS             complement(33000..34310)
                     /locus_tag="PKN92_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47233.1"
                     /translation="MHYAFSDYLLNTYSDLNPAKAPDEIEHAAFLRANLEEDKGATPL
                     THAFNPLTGRGFLWKHGFGGSALEYGKGIAKEMQGQSGPLLWASAGRIFHLLQDMTSP
                     CHVHSIWHPFNISLYEAYWTDFQEQVTDLLVAAPAESLLPDQCALTEDWHLDAFTEAR
                     LLARISALPVSVEGFIKSLAWHAYYASSYWGALQFSDNAAPPEIPPLQSSDGSTEQSD
                     NALRTAFNGSIQLCTSWHGCYFKIKDRKGKAFYWNKCFLLDTFRPCANNGCKSIDGHK
                     WLCKKNAPEGVATIAGRFVFLQRGFFAEHCYPPKYADGAPMEKHLSKYHGDILIPACM
                     RYGLGFLDLFTSEHQAAAQSSLSSPALEQFQKNSPLPYTAPGSAGLLPFTEEQRPSAS
                     DKPPANTSAFDRKANPDKLEAFQNALESALGFLGRGCIPLAPGD"
     gene            complement(34395..35357)
                     /locus_tag="PKN92_00175"
     CDS             complement(34395..35357)
                     /locus_tag="PKN92_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47234.1"
                     /translation="MPAIIAFSPDHFSAGSSLSQKPDITDTPVFVHNASEQSQVRALP
                     KRTPLQQSRDAFFSDAIPCNADQLRAFAQNRAETVAFVQDALASGDTEQQFQALRLMQ
                     DFPLQEMQSVLENMALNEENDAALRMAACYGLKHFTLGTETVHALFQTALATENVLLS
                     RSALIALAGSPVSENTSSLSTLLTHPDPLTCIYALRLSLSDTNDFPFSSFDTLGKTES
                     PSVRCEFYEAVGSAGTKEALAYLKTKAEEETEEAALSVISSEIQFLETKMSAKDEALI
                     RLLADKAASLSIADQHWAYDKLMDMFSVEPMLYRFISWFPPLWQ"
     gene            complement(35469..36575)
                     /gene="ugpC"
                     /locus_tag="PKN92_00180"
     CDS             complement(35469..36575)
                     /gene="ugpC"
                     /locus_tag="PKN92_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNZ47235.1"
                     /translation="MAQVSLHNLSKSFAGNIQALQPCTLHIEDGAFVVLVGPSGCGKS
                     TLLRLIAGLEESTSGDIFIDGARMNGVPPKSRDIAMVFQNYALYPHMSVYKNLAFGLT
                     MRSIPKNIIDERVREAASILGISDVLDRKPAALSGGQRQRVALGRALVRNPKVFLFDE
                     PLSNLDAKLRVQMRTEIMRLHGRLKSTMVYVTHDQTEAMTMGDRIVVMHQGKILQDDT
                     PLTIYQQPANRFVAEFIGSPAINFFPGTLEISGSALVFKSDHFLNNIHLPESAKGAFF
                     SSASNSALLGIRPEHLHVPTEATAEKECFVQGTIDLVEMLGPESILHLDTGKQMIRAR
                     VPSSCNYSSGSSVCFSFQVSDAFFFDGVHFNRLF"
     gene            complement(36665..40237)
                     /locus_tag="PKN92_00185"
     CDS             complement(36665..40237)
                     /locus_tag="PKN92_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ47236.1"
                     /translation="MAYKLSTPAPEKTFQPKGFWALMATQFQGAFNDNVYQWVITFYL
                     LALLQPSGPGTDSSLFFGKFSADTFVPAFATFLFSLPFIIFPALFGALADRFSKQRVA
                     VAAKWLEIFIMLAGGIAFLLGWTPLIWFLLFMMATQSALFGPAKYGILPEILPAERLS
                     WGNGVIQMGTMLAIIMGTGLAGPLFLLTKGKVYLVSVLLVFLSIAGTCFSYFITRPPA
                     ANPAQPIPKNPFLPWQGMLKYFKAIRKDKILFYAVVGYTYFWFIGALARQNLIKFSQT
                     DLLISEDKISYLLAAIAIGIGIGALAAGYLSRGKVEIGLIPIGALGTMFFSLLLSLPY
                     SVHRTVVAEPLIRFAAFMKPGGSCPVIGTLHSLGGHYLTVMLLFLFLGIFAGLYGVPL
                     AAAIQQRAPHGMKGGVIAAVNMLTWIGIALSSVVFLLLNLFGLTAYHIFIFMGVSALG
                     IGLLQCWRSPVMALRMLWWCLDGTLVRLHVTGRSHIPEDQGALLLSTHESFIDLMAIQ
                     AALDREIYFVIGKDALEVPWIRRLSRSMYLIPVDPHAEDDMAAAVVKMRTLLAQGNLV
                     CVNRARSLAPDGVSLPWFSDYSIITEGTGAPTIPLALGRICEILYAYENRKVRLFFSE
                     FRSPIYLNVGEAMPEGITAVKKRYFVQKATAEGFSPRRFRDSVLQYGFIRFARRYPWR
                     PCYADQLSGQISYFKALVGVIVLARKLKPLCGSRKTIGVLMPSTVAGALANIALQVMG
                     KIPVNLNYTASSEIIADCAQRCDITHTVTAKAFLSRVPVTPPGVPVYLDDIKDTVTAS
                     DRIIAMLLALLMPRPLLTRFLGAAPCSEHDIATIIFSSGSEGIPKGVMLTHRNIITMM
                     HGLREMVPHDKHTCLVGFLPFFHSFGYAVALWTPLLEGIQGVLHPNPLEPKAIGQLVQ
                     KYKGTFMIATPTFLQGFIRRCEPEQLSSLRCVIAGAEKLPERIQKAFLEKFSIEPQEG
                     YGTTECAPVVSVNMPAEESPGFYCEHSRRGSIGKAFPYQQVMVVDPDTGEELPPGEAG
                     MLLVKGANVMLGYLHDEKRTQAAIQDGWYRTGDIASIDEEGFIFITDRLARFSKIGGE
                     MIPHIRIEESLHQLLNLTEQAIAVTGVPDEGRGEKLVVLHTLDEEQIALLKERINGST
                     LPNLWRPRAGDYYQIAEIPVLGTGKIDIRQLKNMALMLTEKRSG"
     gene            complement(40413..40880)
                     /locus_tag="PKN92_00190"
     CDS             complement(40413..40880)
                     /locus_tag="PKN92_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNZ47237.1"
                     /translation="MSASINKELELETPIEDIKHELVLNVVRTATTIATRGATLFRQF
                     GLTEAQFNVLFALKYKKKDWTQSELGRRLVVTRASITSVLDKLESKDLVRRNEVSGNR
                     RIYHVSLTTKGRRLINEVEPVYRAEIHKVLRDFDEEQCRRLIDLLEEIRVSST"
     gene            41110..41772
                     /locus_tag="PKN92_00195"
     CDS             41110..41772
                     /locus_tag="PKN92_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47238.1"
                     /translation="MKYFLTAVCCWTALFLAACNEKTFKNEIHSLHKVPPEERIFPPA
                     LSTRERLGLGDSAQHHPMMMQSEEPEGESEHQLKWEAPVSWEKRPAQAMRLVTYAPKG
                     SVSSECSIFILSGPAGGLEANINRWRSQVAQPPLSDEEIAALPRLALLNNEAVLVEMS
                     GVLTNGKGEKVPSMIYGLVCPYEESTLFIKMTGPVDEMQNEKENFHSFADSLYSDEDE
                     TE"
     gene            41769..42914
                     /locus_tag="PKN92_00200"
     CDS             41769..42914
                     /locus_tag="PKN92_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNZ47239.1"
                     /translation="MKTLKKILDFCASYGVAAVLLLLLLLLVFFGTIEQARIGLFAAQ
                     QKYFNSFFLIHYIGGVVPLPLPGGYLLLALLFFNTLLGGIIRAPKRWSRPGLLIVHLG
                     ILYLVLAGFVAHKYALSGNLPLFEGRSASYFQSYFDWDLFITVGGADGSGQQYVVPQE
                     SLAHPGAFFYAEELPFIVKIDRYYKNSMAVEDNSAHAIDGAALKELSENQSAEANIPG
                     MLITLEGDISEKGILWGMQRGPWIVEADGKQFSFDFTRRRWELPFTVTLQKFNHEWHP
                     GTSTPSHFSSRVSVKDEGLPRESLIQMNEPLRHRGYTFYQASWGPSDGKEGDPLYSVL
                     AVVKNPAGQWPVYASCIISAGLLIHYLQVLLRYLKRRSRLSGEKGTC"
     gene            42914..44884
                     /gene="ccsA"
                     /locus_tag="PKN92_00205"
     CDS             42914..44884
                     /gene="ccsA"
                     /locus_tag="PKN92_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNZ47240.1"
                     /translation="MPHFTLFSRSLIFLVMVSAFAGSVFFSQEAGASSWNKDLVYLFK
                     TLPVQESGRIKPLDTAARFMLLRMNGKRTVSVNQGGKNISLSATEWLLDCLFHPEAAR
                     EYAHFSVDSYELITAIGLTPHGKKRDRYSYSELEPALDTLFALAHEVSARPENIRSLL
                     DEQLIALANNVLLFDELTHFMDFARLRFDVAKSALLTLLFPEDHEVPLSKVVEKLPDQ
                     LKSLVDQAADMDEERKREIAKDLEAVFQRLDEVSASGQMLALFPPLSREEKEWMTPAK
                     YLESAFIGMPADTVSGDIALFETLADAAPDSGAFVSAVKRLHGRITQEAQARGEYKHI
                     PLEVHFYDARYLFFSQWLFVLSFFLMAFSWTRSPDSRLARVLSFSILPPLILLGISIT
                     LRCIIRGRPPVTTLYETVLFTTFVAVAIGFFLEFISKNKIAVSVSALLGTIGMFFAWR
                     YEASEGVDTMPAVIAVLDTNFWLATHVTTIIAGYGAGLFAAALAHVYFVLHLPLWKEK
                     DSAAMQTLATSIYGVMAFSLVFTLIGTILGGVWAAQSWGRFWGWDPKENGALMIVLWI
                     LAVLHARRGSYIKARGTAVSALILGMIVVFAWWGVNALGVGLHSYGFTTGIWKSLGVF
                     WIVETFFIAAAIGISVLFPRKEMPVQKGSAQP"
     gene            complement(44875..45633)
                     /locus_tag="PKN92_00210"
     CDS             complement(44875..45633)
                     /locus_tag="PKN92_00210"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNZ47241.1"
                     /translation="MSNLEAVVKEAARAIFQTTNAVAVTGAGISVESGIEDFRSPGGL
                     WTRYPPQEYATLDAFYDNPDKVWEMWYDLGETLKKAKPNPAHLTLAELEDMGHLKAVI
                     TQNIDGLHHEAGNRTVIEYHGNAAQIMCPACTRRRPMDLSQRVLGAPRCECGGYMKPD
                     IVLFGEIIPEYALKTSEMLAKGCSVMIVVGTSAQVYPAASLPYMAKEGGAVVIECNIE
                     KTAFSDVVVDHFIQGPAGKTLPLLMAEIHALSQG"
     gene            complement(45684..46958)
                     /gene="serS"
                     /locus_tag="PKN92_00215"
     CDS             complement(45684..46958)
                     /gene="serS"
                     /locus_tag="PKN92_00215"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNZ47242.1"
                     /translation="MFDIRLIRSNPELVRAAMEKRNAPVDLDTILAVDARRRSAVYEM
                     EQLRARQNKASETIAQRKKNNENADDVIAEMKQIKEESANLDLIAKQAEEDLEEILLS
                     LPNLPDESVPAGKNEADNQEIRRWGEIPSFNFEVRDHVDIAEKLGIVDFSRGAKISGA
                     RFTLSLGYGARLERALINFMLDIHTQKHGYQEALPPFLVNTASVQGTGQLPKFSADLF
                     KIDNTDYWLIPTAEVPVTNIHRDEILSLEDLPKYYVAHTPCFRSEAGSYGKDTRGMIR
                     VHQFNKVELVKFTTPESSWEELEKLTANAEYILQQLGLAYRVVCLCGGDLGFSAAKTY
                     DLEVWVPAQNTYREISSCSNFTDFQARRANIRFKRDKKPEFVHTLNGSGLAVGRTVVA
                     ILENYQQEDGSVIIPEALRPYMGGLSVIPAQT"
     gene            complement(46973..47899)
                     /locus_tag="PKN92_00220"
     CDS             complement(46973..47899)
                     /locus_tag="PKN92_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNZ47243.1"
                     /translation="MTDAPKKKTTKGLGRGLNALLPSNVMENPIPPEQGISDVRRENF
                     SGPASGVLMHLNPEEIKPNPRQPRKHFNEESLGELAESIKNDGVQEPLIVRFNNGSYE
                     IVCGERRCRAAVMAGLQKVPVICRDISDQEMLKFGIIENIQREDLNAIELAQAYKDLM
                     EEYGWTQEQLSVEVGKKRATVTNTLRLLQLSDGLQQLVADGSLSMGHARALLSLSTAS
                     AQQAAAQKIISQGLSVRQTEQMVAKFGEKKTQSVRKKNEKEPYMQELEERLRRGLGTK
                     VNLKPVDNSRGTIEIAYFSLDELDRLLEHMIR"
     gene            complement(47892..48650)
                     /locus_tag="PKN92_00225"
     CDS             complement(47892..48650)
                     /locus_tag="PKN92_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ47244.1"
                     /translation="MTRIIAVANQKGGVGKTTTAVNLAACLAELGSNVLLIDLDPQGN
                     ASQASGINKSTVPATIYQALTGHVPIDNAIVQTPYKNLSLVASTTDLVGAEIELAQLE
                     NRGFYLANTLRPAVSRFDWIFIDSPPSLGVLTLNAMTAAHGILAPLQCEYFALEGLSE
                     LLQTIINVKNTLNSHLNVFGVLLTMYQHTVLSRDVEADVRNHFMGKVFKTVIPRNVAL
                     SEAPSFGMPINHYSPKSTGAQAYGALAKEVLIRD"
     gene            48903..50186
                     /gene="hemA"
                     /locus_tag="PKN92_00230"
     CDS             48903..50186
                     /gene="hemA"
                     /locus_tag="PKN92_00230"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNZ47245.1"
                     /translation="MTIAITGLSHHTSPLALRERMALPVERIPAMLQDFNARFGADSG
                     CFILATCNRLEVYVHARQNTEAIHREITAFLGKATRLEESEFSDFLYHYSNEAAVKHL
                     FSVAASLDSMVVGENEIMGQIQRAHDLASEAKTLNRQLSMLIQRSLKCGKRVRTETAM
                     GKGKVSVASVAVDLAEAKLKQLTGKTALIVGSGQVSELALKSLVERGVERVLVLNRTL
                     AHAEELASRYQGEALILQALPCHLHRADIVISSTGASDIILRAVDFQRAIEKRGRAPM
                     FVIDIAVPRDIEKEAGRIDNVYCYDMDDLEGVAERNLRKRQDEIPACLEIVDKESLSF
                     LQWQRRLSSDPLIKSLTQSFNAIREKELARTLSELPGLSEKEREKLEQFSKRITNTML
                     RMPLRAIKEFSQMPEHELLVEHIQTVFGWGDAKVE"
     gene            50183..51865
                     /gene="hemC"
                     /locus_tag="PKN92_00235"
     CDS             50183..51865
                     /gene="hemC"
                     /locus_tag="PKN92_00235"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNZ47246.1"
                     /translation="MKIIFGTRGSALALAQTEQIMALFSALHPAVEAEMKIIKTSGDR
                     DQHSSLSSLGGWGAFTRELEKELIAGTIDAAVHSLKDLPTEQPEPLTIVAVAARAPVE
                     DVLLTNEDFTLDTLPSGAVVGTSSLRREVQLRRFRPDLCIKDIRGNVQTRIDKLRRHE
                     YDAILLARAGLSRLALLQEKGLYIIPVTMMIPAPGQGAIAIEIRKSDNLLQDLLLPLN
                     DEQTAACVQFERSALRSFGGGCRSPLAIHAAPHASGLEFQSFVAESRNRYFFFHKALS
                     SWNTVTEEGDALGQMLQSVSVRTESAHKTPEDALSDKKILVTRAHHQSKPFCDILRTH
                     GAEVLELPFISIQGVAFEKPSLKDSWLIFTSANAVHHFVNPEGTDPASCRFERDLLIG
                     AIGTGTAEALSEYGVTTPYIPQKGTAKVFANELTAKKKMSGITKAVIPCGDKALPELS
                     ELLVHQGWTVETPVCYRTVIARLTEGEKQAIINFRPDVITTFSPTAVESMMQNGIPEN
                     LQKVQCAPLYASIGPTTTQELQRRNRTPVLESFLQSGTGLCDALCAYYEGLH"
     gene            complement(51875..52339)
                     /gene="smpB"
                     /locus_tag="PKN92_00240"
     CDS             complement(51875..52339)
                     /gene="smpB"
                     /locus_tag="PKN92_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNZ47247.1"
                     /translation="MKEGKKFITKNRHAFHEYTVLDSFEAGIVLEGTEVKSIRMHNTF
                     SLKEAYVDFTENEAWLVGSRISPYDKGNRNNHEPVRRRKLLLKKMEINRLIKRVEEKG
                     LTVIPLSAYFKQGKVKVEIGLCRGKRLYDKRESIKQRETDREIERAVKRYKT"
     gene            complement(52336..53973)
                     /locus_tag="PKN92_00245"
     CDS             complement(52336..53973)
                     /locus_tag="PKN92_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47248.1"
                     /translation="MSNSVQLTFWGGTASKPKIQRSSSGKPFLFIIFVLIVLASVWFF
                     IPARHAAEMESFIPASHSIEVYVYDPATKRNELLKSPILHTIPEDTAAAAYLHLLTRK
                     LPVPDWLLNNVSSGLFHISVEDSNRPDSLLVITRMTRIGLLSEKFFSLSGNVSRERAG
                     GLDLKKVINAEIFYSVRNKYLLVSSSRDALIQALTLPPEEAIEKSKFEAAQQKAKEAD
                     LFCRLDGKAFLQSPALFKDLSFSVRLNQSVPRLSITGSFSDEILARYGTLLPQNTKDD
                     SLDMPLDGIASLSVNLGAPLPQWYAKLAETNEGWEKVSSWITLSESLPENASATELLQ
                     YLIASFFHSSGEKIRLAWFGFDPLEVVPAPLLAGTAQGKTDALHAAFDLVPPPPFSDD
                     MIVLNPSVNSELLLATVPFIGGENLTPSLAIYPQGALFATSETLIRKLMQSEALTAQL
                     GQKANLYFHLRPAQAWEAAVPALNEFAASGLLRGHTESSFAEAATKISKQISTLQEFT
                     VLGIVSDKAFHLEAKVTIGLSDQADNNAIQIAQRGQR"
     gene            complement(53966..56404)
                     /locus_tag="PKN92_00250"
     CDS             complement(53966..56404)
                     /locus_tag="PKN92_00250"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNZ47249.1"
                     /translation="MKNRCPLSIPFRLFRLKVNFLSRGTALYFAAFLFILLSAVQSWA
                     LEAGSAKIDITPPVGTPLNGYGDRMGRNSTSVHDPLWARTVFLDDGETRFFLVSLDLV
                     AVNPELRQRVLELYSGPVPKEHVLLTATHTHNGHGGMCQKLMIRNVSGRFIPAVLEAT
                     AEKIIESMHKAYNGRRRAALGYAVGSNQGLSVNRRFSAGPAESQIGVVKIEDSDGAAI
                     AVITNFTGHPTSVGGQDKYSFSADYPGYYYLEMEALAEESCVPLFLNGAQGNQTIAAP
                     KGKSGWDRTEAVGRQVAQIADALSKNMSFGDAVFKLSHTKALLPLTLAENLQPKEVDL
                     YSLEINDLLISFFPGEPCVEIGLNLRERALARGYDAHWSVGLANDYLMYFVPRALYPF
                     ANYESFMNFFGPGMEDWLYSQFESLMSRKTLVESIVDLPDEEAQESQQSEGDASFPLL
                     TLHGSSHDIGLARGSFFHNAIQNKYKTKVLDPVDTGQWYPTGSWWTAMPSFLNLTPLA
                     MTALGMGSRSLLQGLSESYIREMEGMAEGAGLPFDGLWLLQHARLYSLAKDQSSLFSL
                     PLCTMVALTSEYTGTKDILLGRNLDWKGDENDYVIRMLPEEGLPSIQVGFDWNAGLMT
                     GLNRDRLFLCLEYVPYVDTVLPEKAPLEFLLGQLLRSCSSFSEALKVLSAAEYIKNYH
                     VLIAGYDGEKADAAVVEYGQSISVRKAVKGILTGVNPDTQGIPDETRERYQALIETLK
                     NQPVKNRESLGTLLTASPMESDGGMQHIWNHASRHCVVLQLSQPEIMCAFKQPDGKAG
                     SFATLTVSEISSHE"
     gene            complement(56434..58623)
                     /locus_tag="PKN92_00255"
     CDS             complement(56434..58623)
                     /locus_tag="PKN92_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional SDR family
                     oxidoreductase/aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ47250.1"
                     /translation="MRLLITGGGGYLGYWVVREALSKGHVVRVFDRFSFLSEGMTPET
                     LFAENNFGRNLEIIPGDIRRLQEQQNLLYGIEGVIHLAGLSSDPTCALDPEIAYEVNV
                     ECTRELARQCIQSGIRPFVFASTCAVYGQGVFPILDEESPVNPVSVFAETKIEAEKAL
                     LDMSSAQFTPRVARMGTLFGISGRMRFDLAVNHMTASAVRNRSILVKGGGNQWRPFIH
                     VRNAAQILVSLASDTDLSGKSGIFNVGWDTQNCTIAALAEKVAARCSQVPVTTARDDD
                     DQRNYKVRFSKIAETVPLPEQISFEEGIDELKVYLDNPDINPFSEAFFNVERMKKLRA
                     TPVDAGGEPVAARFIALSKPNLGEDEERAVLQVLRSGWLTSGPQITFFEKSFAEKVNA
                     PYAVSVSSCTAALHLCLAALAIKPGDEVITSPITWASTGNTILTMGATVRFVDVDPHT
                     LNMDPLQLEATITEKTRAIIPVHMAGLSCNMDPIRAIAKRHGIPVIEDAAHAFGSEYR
                     KKPVGAEGLACFSFYATKNITTMEGGMITTDDESLAHRLRRLAGNGMGSSAWDRYGRS
                     AVSGPAEVLEPGYKYALSNVSAALGVEQLKKFEQFRAARKRIAELYLSVLSDVPELEM
                     PPDAEDGRHAWHLFIIKIRPNTLSVSRNSVIEALRRENIGTGIHFYGLHLHQYYREVL
                     GMRPEDFPQATDVSDRIISLPLHPGLNDRHVQEVVSALKKVISYHRL"
     gene            58906..59370
                     /locus_tag="PKN92_00260"
     CDS             58906..59370
                     /locus_tag="PKN92_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNZ47251.1"
                     /translation="MRVSKKSDYALRVLVYLAEKIGARPVSIRTLARLHDIPYRFLQQ
                     IVLDMKEQGWIMTLPGREGGIQLAKEPREITMGEVVRCFDGVLAPIACVSITQNEACS
                     QEAYCKFRRTFLEIRNHTAQRMDKTTLHDLTFCVPLSQEEVFGVQFKDGAGI"
     gene            59382..60467
                     /locus_tag="PKN92_00265"
     CDS             59382..60467
                     /locus_tag="PKN92_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012068685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ47252.1"
                     /translation="MPFVRLISLCLFLLFLSGCFSYSGKDTAVEILNVSYDPTREFYR
                     EYNAWFEKKAFERTGKHVRILQSHGASGKQARFVIDGLEAHVVTLALAYDIDAIAQKS
                     GSLSKDWQTRLPFASAPYTSTIVFLVRKGNPRNIQDWNDLIKPGVQVITPSPMTSGGA
                     RWNYLAAWGYAWLISLEKRGVILSAITEDERRQADEDAFSFVSALFRNVPVLDSGARA
                     ATTTFARRGIGDALVTWENEALLAMKSLPGKALEIITPSISILTEPVVAKVDEVTARR
                     QTDALAQSYLEMLYSPEGQAMAARHHFRPRHMEFVNAEDKNQFPDLHLFTLEELFTDW
                     QTAHKNHFSETGSFNKIYQSLYSLENQ"
     gene            60464..61303
                     /gene="cysT"
                     /locus_tag="PKN92_00270"
     CDS             60464..61303
                     /gene="cysT"
                     /locus_tag="PKN92_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNZ47253.1"
                     /translation="MIRQIKQKHFLPGFGIGLGFTLSYLSLLVLIPLSILAVKSLSLD
                     WTVYRSLLIDPRTYAAIKLTFLSSLVAAGINLVFGFIVAWTLVRLPFPGSRLIDALID
                     LPFALPTAVSGIALTSLYVRTGLLGRYLEPYGVQAAFAPLGVIIALTFIGLPFVVRTL
                     QPALQDIDKEYEEAAISLGASPFRVFASVIFPSIRPAMLTGFSLAFARALGEYGSVVF
                     ISGNMPMKTEIVSLLIVGKMERFDYEGATALAAITLLASFLILLAINLTQWHYSRKVQ
                     QTG"
     gene            61308..62183
                     /gene="cysW"
                     /locus_tag="PKN92_00275"
     CDS             61308..62183
                     /gene="cysW"
                     /locus_tag="PKN92_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNZ47254.1"
                     /translation="MDTDMRSSMQRLIQFFMRCITITFVVLFLVLPLYVILSEATGEG
                     MSVFFRAVTHQETLQALTLTICTLFIILPLNILFGIAAAWTLTKYEFRGKSVLSALID
                     LPFSVPPVIAGFILILFLGRHGTLGFLLEKLDTQIIFAAPAVIVATLFVTFPFVAREL
                     VPLMQEQGVDEEQAAVSLGASAFQTFFHVTLPNIKWALFYGIILSTARAMGEFGAVSV
                     VSGHIRGKTITLPLHIEILYNEYNFTAAFAVSSLLVTLAMSTLVVKEIVYWRSGKKQT
                     RPTGGFLESPETPDL"
     gene            62180..63256
                     /locus_tag="PKN92_00280"
     CDS             62180..63256
                     /locus_tag="PKN92_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNZ47255.1"
                     /translation="MSIEVQHISKSFGSFHALKDVSLRVKSGELMALLGPSGSGKTSL
                     LRIISGLDRPDVNPLAKVIMNNEDMISTSAGNRRVGFVFQHYALFRHMTVFDNIAFGM
                     RMRRKKERPSKAEIKNKVEQLLHLIQLDNMGNRYPLQLSGGQRQRVALARALAIEPKV
                     LLLDEPFGALDARVREDLRRWLRRLHKEIQITTLFVTHDQEEALELADRVVVMNEGRI
                     EQIGTPEEVFHSPRTEFVMKFLGSVNLFHGRIERGKAVFGSWKVSGPEAGKEEGTPAR
                     LFVRPYDMTIVREGDPAHGSNAVIRRIQSAGPYVKIELEHESGENIIAEMSHERHESL
                     KPLVGEKVAVSLKNVRVFTEDYTI"
     gene            complement(63262..63753)
                     /locus_tag="PKN92_00285"
     CDS             complement(63262..63753)
                     /locus_tag="PKN92_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ47256.1"
                     /translation="MACVLLNITSVPLRITLEVMLHLEGHTLSEIAGDAAVTDSLPHA
                     ADLAGQYPVLLLVPAAQVADAVGLMRKGVHDYALLPLQPGEVPFKIERILASLSAPAH
                     DGVIETGQELKSLAAMEEAHIRLALRHCKGNQLQAAKLLGIGRNTLWRKLRRYAAQQS
                     DDR"
     gene            complement(63784..64029)
                     /locus_tag="PKN92_00290"
     CDS             complement(63784..64029)
                     /locus_tag="PKN92_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47257.1"
                     /translation="MIKKEFTLNARLALLSLSIGKARLENNCATSGLPAQQRQEFSRV
                     LDSILADYEQLWLARNRSGGLSDSLEKMKRLAKLLEQ"
     gene            complement(64171..65565)
                     /locus_tag="PKN92_00295"
     CDS             complement(64171..65565)
                     /locus_tag="PKN92_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNZ47258.1"
                     /translation="MRTETTRIPVDINATAVVKDRKPPEPGSLVLLPQPRLIRSLNGS
                     NLFPAKEPAVRFDAKTLKQQGYTLDISEKDITIIAADEPGLFYARQTLAQIQRLYAGS
                     GRIPCLHIEDWPDFPNRGVLLDIARDKVPTMQTLYEYVDLLAHLKFNQLQLYTEHSFA
                     YPGHEVVWKNASPVTPDEIRELDRYCAARYIELVPNQNSFGHMERWLAHPEYSSLAEI
                     PGRSDLCPTNPGSIALLKSMYDSLLPCFSSSQVNVGCDETFTLGEGASKAEAERLGKG
                     RVYLNFLAQIYDLVQGHGKKMQFWGDIILNHPELIPELPKNIIAMEWGYEAKHPFDER
                     CAQFARSGIPFYVCPGTSSWNSLLGRTDNALANLERAARNGLNQGAIGYLITDWGDSG
                     HWQFPPVSYLPFAQGAALSWAYDANRSLDLVKAADLHIFEDASGLLARSAFDLGNAYL
                     QTGTSRDNAAVYYG"
     gene            complement(65695..66450)
                     /locus_tag="PKN92_00300"
     CDS             complement(65695..66450)
                     /locus_tag="PKN92_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47259.1"
                     /translation="MEENTESQPVILGTPSYQGLLYGKEAGQSFDLWLPQGHPLPCPA
                     VFFLKKTASDAFPALFFKNCLRQGWAVVAISLQRDVPAELLQESTENQEPTNLLDNLK
                     TALHFFSEQSVAHGIRPDRIFLVAFDELAPYALYGNCFEANSERPETKINACALILSK
                     NIQTEREIQESLDELLAENHSIENVNIFFAGSNFLEWLETLLADHFSSLSQHLLAEGI
                     KDAQISELLISTFQKRIKESSAQKKKPDSSNSD"
     gene            complement(66714..67742)
                     /locus_tag="PKN92_00305"
     CDS             complement(66714..67742)
                     /locus_tag="PKN92_00305"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNZ47260.1"
                     /translation="MELTPKMIFVIGAPRSGTTMLERIISSHSAVQGGPEPHIITPLA
                     HTGVWDKVDKAPYDHILAAEAQKFFVSQLPEGENDYYAACRAYCDVLYGRFLQAKGTK
                     LYCLDKTPAYALVLPFLSRVYPDAAYIVLTRHPLAIFSSFANSFFDGSYQEAHAYNPV
                     LERYIPAMAAFLRNPSTRKLHVRYEDLVKAPEEWFEKICNHIGIPFEAEAVNYGEHRD
                     KQQTGGLGDPIGVSQHSRPTTASVKKWAAEIHADPLKLTLAKKMIAALNPEDLALCGY
                     PLENLWQPLVQIEGTTLRKPPRSKLTRYSLERKLILFFRRRAQKNKLFRKLLLKIRLI
                     ADVFLREV"
     gene            67900..70815
                     /locus_tag="PKN92_00310"
     CDS             67900..70815
                     /locus_tag="PKN92_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNZ47261.1"
                     /translation="MPFSQRVVALGIRHPRAITASMVISALLLIAAVAIPTLLPGSLP
                     LPPVRVDTDPENMLSRTEPVRLYHDSMKKVFDLNEIIVVGVVNDSHPEGVFNPETLRQ
                     VYELTEYAKTLRGEVIGLDDPMAGVVPMDIIAPSTVDNIEQGGPGEVKFEWLMKEPPA
                     TEEEALAVRDRAAKIPFLQGTLVSESGKALAIYLPITSKDLSYRIYSKLQKKIAEFPG
                     AEQWHITGLPVANDTFGVEMFIQMAISAPLAMLVIFLLMLYFFRKFVLILAPMLLAVY
                     VVVVNMGLLIVTGNTIHIMSSMIPIFIMPISILDSIHILSFFFDRYQKFKDRRKAIEH
                     VMDTLFSAMLYTSLTSAAGFASLAITPIPPVQIFGIYVAIGIMIAWFFTMTFIPAYIM
                     LLPERTLARFGSMISEEDDSGPDTSTRLGRLLAGAGVFTFRHARIVLVGAVLLGVVAA
                     YGISKIQVNDNPTRWFKHSHPIRVADRVLNEHFGGTYMAYLALQPESTGPELGEYAVD
                     LEARLRRTVDEMNLDEEPKNALLAIASLLHEKGAETQTIQELRQMLHKEADDRLNAAS
                     EALMTADPEDSVAESLVEAWEDLLLFLDMEEQRNEIFKQPEALAYLEKLQNKLLETNV
                     VGKSNSLTDIVKTVYRELMEGNADYFTIPETARAVAQCLITFESSHRPQDLGHFVSHD
                     FRETSLWVQLKSGNNQDMKYVTEVMDAFIAENPPPFGLKHHWFGLTYINVVWQEKMVI
                     GMLNAFLGSFLIVLLMMTVLFRSGLWGLISMIPLTVTIALIYGVVGLMGKDYDMPVAV
                     LSSLTLGLAVDFAIHFLSRSREYHDLKGNWEGAYPLVYGEPVRAITRNVIAVAIGFTP
                     LLAAPLVPYNTVGFFMAGILACAGLSTLFFLPALIKVLEKLLFPQDKICRITCRCGTC
                     VVTAVIATATVALNVQQFLDMTWTGLSLVSLVVVLASGTVCFFMSRRESCKTVSDEK"
     gene            70852..71649
                     /locus_tag="PKN92_00315"
     CDS             70852..71649
                     /locus_tag="PKN92_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNZ47262.1"
                     /translation="MIKHAKMMLLATCLITLTCSALAQDAPSVDEIIKKANHAAYYQG
                     SDGRAKVKMSIKDKQGNVREREFFILRRNADDKDEEQQFYVYFNSPADVREMVFMVWK
                     HVGKDDDRWLYLPALDAVRQIAATDKRTSFVGSTFFYEDVSGRSIEEDVHELVETTDN
                     FYVIKNVPKNPGSVEFDSYKVYIHKTTFIPVKVEYEKGGNIYRVGEAQKVDTIDGFPT
                     VTKNVMKDLNSGSETTIEYLKVDYNVGIPENIYTERFMRRAPRQYLN"
     assembly_gap    71833..71932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            71949..73289
                     /locus_tag="PKN92_00320"
     CDS             71949..73289
                     /locus_tag="PKN92_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47263.1"
                     /translation="MGLEPSLPAGLDSVGERTQEPALPSGLEPALPEGLGAAPFNEEN
                     DSASALRKESPLQFHGFWDVRAGSRITSDPAQAKDATLGEMRLQLKTDKDWDRVFIEF
                     TGDALFDAVEERARIDLRQLRLTWTPVESLDIRIGRQVLTWGTGDMLFINDLFPKDWR
                     SYFSGRDVEYLKAPSNAARIGWFNEIMNVEVVYTPRFNPDRYITGKRFSYFSPMHGKT
                     VGRKQQVHTIEPDDWFDNDEIAVRLYRNLGSAQLALYGYSGYWKSPGGMQLFPSRAIF
                     PKLRVYGASIRGALGKGIANLEFGYYDSYQDRSGSSPFINNSEIRFLAGYEREIGHEF
                     TAGIQYYLEYMQQYKAYKKTLPFFMKARDRDRHLITLRLTKQLLQQNLILSLFAYASP
                     SDSDSYLRPNVSYKINDNWTVDFGGNIFMGRQRHTFFGQFEDNTNVYAGARVAF"
     gene            complement(73378..74361)
                     /locus_tag="PKN92_00325"
     CDS             complement(73378..74361)
                     /locus_tag="PKN92_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47264.1"
                     /translation="MVKIGIMSFAHLHANSYAACLKALPGVSLSAIWDDNASRGKKMA
                     KEFKTNFVKDMNEFLASDIEGVIITSENSKHRAMVEAAARAKKWILCEKPLAPTMKDA
                     QSIVTTCRKAGVGLGTAFPCRFSTPLIEAKSMLNSGKFGAVYAITCTNHGQYPGGWFS
                     DPALSGGGATMDHTVHVVDLLRWMLGKEFKSVYCELGNQLHNKSFPTDDLGSLHLEME
                     GGIQVSHLASWSRPKSFPTWGDVTLEFVCEGGLITVDAFNQKVTVFDDGNQQVRYDAW
                     GDNPDMALVKNFVDAVQSRCDCASTGMDGYAATAVTEAAYRSAKTGRTIKI"
     gene            complement(74377..75351)
                     /locus_tag="PKN92_00330"
     CDS             complement(74377..75351)
                     /locus_tag="PKN92_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47265.1"
                     /translation="MNVAILGCGGMGRLHAQMATNCGLRISYCADAVRKAAKGLADDF
                     GAKAVVDPMKAIEAKDTDIVVITTPTPSHETFVSAAAAAGKHIFCEKPFGRSSEECKR
                     AIRAAEKAGVTLFVGHVVRYFQEFELMRERIQKGSIGKTGWVKLFRGGIFPGAPGSWF
                     RDYKQSGGVTFDCMIHDLDWVRYVFGEPERIFCQTIMRTEPTQLDYSQVTMRMKSGVI
                     ATIINTWAAPSGFKVKAEICGSKGMIQFDSTETPLGAEPRQTGSGPTMFVPMSPVAVS
                     PYQLEWQDFIATIGSGHTPRVTPLDALRAVEMAEAALKSNSSKEPVKF"
     gene            complement(75419..76234)
                     /locus_tag="PKN92_00335"
     CDS             complement(75419..76234)
                     /locus_tag="PKN92_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ47266.1"
                     /translation="MLTGVNQWAFPADMPALDAMELAARLGFQSFELCVGEDGPLPLS
                     TTQSEAAVLRKKAEELGLVLPSLGTGIGWKYPLTTPDETLAKKGIEQTAQALQLAQWL
                     GAQSVLVVPGSVTSDLSYDDAYENALDNLLALVPQAETTGVTLGIENVWNKFLLSPLE
                     MRDFIDECDSEHVGAYVDTGNIILYGYPEQHLRILGHRVCAVHAKDFRASAGNFDGFV
                     MLMEGDVNWPEVMAALKEIGFDGALTAEYGPYQHSTEVMLRHVRTSLEAIISL"
     gene            76426..79584
                     /locus_tag="PKN92_00340"
     CDS             76426..79584
                     /locus_tag="PKN92_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ47267.1"
                     /translation="MNVVTTKNSLVLCLLLVALAAGCGLRLLHIQNESIWWDEYASHA
                     HLDASSFSEFYDLVRTLDPLALPGYLVPEYFWAKWVSPSLFSLRLESIAIGLATLLLL
                     YAFADKYLSRRVALLSVWLLALSPVHIHHSQSIRMYGLLVFLALGVMWSFLGFLERRN
                     LSSILLHGVFSFALCWTHPFAVLVLAGPVTYMLFTRQKQLRLLLIWFLLQGTIFIPQA
                     FYLAKVRFWPQNTTAAWFARPTFTELIADLFFDDIISFHWQLRLGEYAQKLVLFRSAS
                     DWLLALAIVFFLIAAFLFAWKKRKETKQQLVLYCILPWLILPPLALFCASWLLRPCMF
                     PRYTVHCLIPLYLLVAAGAGISRNKLLVSFAALVLLAGTGLQWVWTLPGPQRTQWRQT
                     ALFLQEQSKREDVVLVEEFLWRDVLQHNARWAAQVVLALPVAAVADQKLLAAEAAFCL
                     SRQGKAESVVWAVIPRDYFDFSPPADFESWLSEAGLSFERFCLSAIRDIYIYKIAAER
                     KSENALSLEECFSLDRSFGGEEREKALTHREIESVGDLALELAVQGSREEACTIFEAL
                     MQESSFAKEIYSSVVSALQRDEFFLKRIEAVRWLWKGYGARNNSQTAYAIYCFSKAAQ
                     KDSASGLAHLEEALERIPQRDYESAASALTKAQAQPGSYREITKNLLQALNSGKHIFE
                     CYEAVKSYREASLAVEQGDTAGAKHLLEESLSLDPALALSQLLLAYIRSVYEEGQRVQ
                     EMMEGYAALTGKSITEAYAHLALLQAENFHPDLCKKTLDTLFQADPDTQKIYRSLHKA
                     CSDGKNSRQAAASIRAMWNAYEVMAAGDNEAAGDLFGDAARLDSTNTLALLEWGLLLA
                     EKGAYDEALTALISAADADPDYARFLAEVIQALETGQAPEKHIAAVRAYRRGILAQSR
                     GELDAAEKALKEALFMDSTMTEAYTSLTFLLVMQKKYAEAEEILTHYFSVADTPVSGA
                     YGLMSLLHLAAGREGDAAKCAFKALAGDATYTAQFGVFFTALFKDKNKSAVLEEIHRL
                     KGEGINLEALLGDLLDSYFQRP"
     gene            complement(79653..80063)
                     /gene="speD"
                     /locus_tag="PKN92_00345"
     CDS             complement(79653..80063)
                     /gene="speD"
                     /locus_tag="PKN92_00345"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNZ47268.1"
                     /translation="MHKGRHLLIDCRNVARELCLDDKRVLNVMARAAEKAGATVVSQI
                     RYKFGADSPPGFTCVVVLDESHCSAHTYADLGLIAMDVFTCGNTHPRDVLRYIQEELD
                     LGDVTVTEVGRFSTPGTREYVETYLDQEPALAMS"
     gene            complement(80365..80442)
                     /locus_tag="PKN92_00350"
     tRNA            complement(80365..80442)
                     /locus_tag="PKN92_00350"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80405..80407),aa:Pro,seq:ggg)
BASE COUNT        20473 a        19217 c        20595 g        20318 t
ORIGIN      
        1 gccacttgta ggcggaggcg gaggaggcag ggctgatatg gcacaggcag gcggaaaaga
       61 tattgaaaag ctgcctgaag ccttgaaaaa agcggaaaca atcattgcag aacttattaa
      121 gtgatctgaa aagcaccata taaagccagg ttcgcaggtg tcgcatttcg ttcggcgcag
      181 accgtatgta tttctccgga tgatgccgtc tgagcataaa gactctttgg aaatctgtgt
      241 ccccaactct caggagtgtt attaatgcct tatttgcaat ttgaccgtga taaacttttt
      301 gtaaaaagtc tgtctcagcg caaagacaaa gtgtttatag aacgggatca tgttcctgtg
      361 aacgcatccg tcgccaaacc tttgagcagg cttggtcaaa aaacaatgaa tgaactggca
      421 gacagactga ttctagccaa aaagacgaat aaatcgcgca tgctcacctt cggcgcacac
      481 gccatcaaaa atgggctcgc gcctgttttc atccatttaa tgaaagaagg atgggtgacg
      541 catctcgcaa ccaatggtgc cgggattatt cacgactggg agtttgcctt tcaggggcac
      601 agcagcgaag atgtgcgggc gaatgtggat cgcggtgaat ttggtatctg gcaggaaacg
      661 ggcacttatt tgaattttgc cattgttgca ggggcctgga aaggtcttgg gtacggagag
      721 tctgttggcg cttttgtaga aaatgacggg gtagaagttc cgtcacagga tgaattggtt
      781 gaagaaatac gggagcaggc agcgcaaaat ccggcacagg cagccgctgc tgcggatatg
      841 ctggatcgtg tccgccactt tgatctgaaa cccggcttca tgtctgtgcc ccatccctac
      901 aagaaattcg gagttcaggc agcggcatac cggcttggtg ttccttttac aagccacccg
      961 atgatcggac atgatatcat ttacacccac cccatgaatt atggtgctgc cattggcaga
     1021 gccgcccaga gagattttct ggtttttgcc cagcaggtta tgaatctgga tggcggcgtc
     1081 tatatgagtg tagggtctgc tgttatgtcg cctatgatct tcgaaaaaag cctgtccatg
     1141 tcgcaaaacc tgcatattca ggagggaaaa cacattgaca atcattatat ggtgattgtc
     1201 gatctgcagg aatcacattg ggactggtcg cagggggagc ctcctatgga taaccctgat
     1261 tattatctgc gttataacaa gagtttcaat cgtatgggcg gacagatgcg ctatgtaaca
     1321 gccgataaca gggacttcct gctcacgctg gttaaaacac tggaagagag aaagtagcgt
     1381 gtgatgattg ctttcctgat gctttttatt gcgatggcgg gcagtacagc gaaggcatgt
     1441 gctgcattcc aggtgccgga agtcaggatc agccctgagc atcccctgat tctgcttcag
     1501 agttctgcgg catttggaga tgcggactca agtcaggaac aggcgaaacg agtagaggaa
     1561 gcgcggcgac acggagagga aatcgtacgt gtctggaacc tgctcccgtc ggatatccgt
     1621 cccctctgtc agattcaaat tgagttgcgg gtccaggagc attccttaag aaaaatgctt
     1681 tttgaggaaa tgctcagacc ggtacaggcg aatgaagtgg cggtgagtct tcaaattgca
     1741 gacccccatg atgagtatgt ctttgatctt aatgctgttg aaagcctttt acagaccttt
     1801 ccctgcataa aaagtcttat ggtatctgaa cagcaatttg cccattacgc taagttcaat
     1861 gttgaggact atgctgtccc gcctcatgtg cgctattgca tggatgtgat tcaattggga
     1921 gcccgttacg gaaagcatac tgttctggta ttgcaggggc tcaagtggct tcacatagga
     1981 gccgacacgc tgaaccgccc ccttctggag gcgatgcgtt ctttttccga ttacgtgatt
     2041 ccggtgaatg aacatattga gccacgccat ctgattcgac agacagctgt ctggggatta
     2101 tggctgggtg attttgtgac gcattggggt gttgagcccc aatcctggtg gtttgaaagc
     2161 agcttcatga atacgccggg gattttcgga gatcatctgc accctgcgga gatgcctccg
     2221 gaaatgtatc gccccatgat tctgcaaggg gcggcgatgg gtgccacagt ctacagcttt
     2281 gaaccgtggt gggatctctt tgattacggc aacagccggt gctgggatga agtgattctc
     2341 ccgacgcttc gggaagtcat tcagagcgaa cttatcccgt cgaaagaaca ggtgctggaa
     2401 aaaacgccgg tcgcctatca attagctccg gctaggtcta ttcatgattt ccacataaat
     2461 atgcgcgatc tcgactggct ttctgatgac ggtaccttgg cacagctggt gtacggcgtc
     2521 tgggagccta tgttggaatt tgagttgatt ccgaataaaa gcaattggtt tatcccgctt
     2581 ttgcctgctg ttgtttcgga ggaggcggca ggacgctttc cccgacttct gcatgcagga
     2641 gattgcgatg atgaagcctg ctggagagag cagctcagcg gttatctgaa agagccggca
     2701 tcgattccgc aggcgtggac ctgtgaaatc aatgatcatg cctatgtcat gcacacccat
     2761 gaaaatctgt acgaaaagca gttctttgaa gtggaaacgg cgcagcccgt tcggaatatc
     2821 actgcgtctt taacagagaa tggctccttg cagttgaact gggatgaggt gcctcaaaca
     2881 gccagttatg aagtgtgctt cacgtctgca aagggatcaa ccgcagtgct ggcgactgtc
     2941 agtgaaacca gttatatagt gaaccttccc gaaccgggaa aattttcagt tacctgtact
     3001 acccggtcaa gagaaagata ttccggttct gtcaattatc tggactgtct ggttttcagc
     3061 cagcgtacaa gccggcccgt ggagcatatt ctctttacga aagagggaac agtgctgaat
     3121 gaaaaagagg aggcggttac agatacccga ccggaatctc agcagattta tcctgattat
     3181 tccggtgtgc ctgaccagtt tcagcgtctt gccgaggaag tgacaggggc attggatgaa
     3241 tttaaaaatg cctacgagtc catggactgg aaaagactga cagcgcttta cgacccggcg
     3301 tatgaggatg ccaatggctt tcacagggaa tatgtaagcc gggcatggaa atggtggttc
     3361 cgcagaaata acaaatgctt tctgctgcgg cagatcagga actgggattt ttcagagtac
     3421 agccattcag agattgtcaa aaacatgctt tttctgtatt gcaccgctgt tcgctgggac
     3481 gatcagcctt tcgggtatga cggcattgtg cgtataccac gccataaggg tgcggaagtg
     3541 cagtgctcct ggataaaaaa agagggcagg tggcgcctgc tcaaaacgga accgtcgctt
     3601 ccgaatctgg aagaaattct gtggaacagc cgacccatgg acaaagagga aaagctggtt
     3661 cccagtctgg atgaatgaca gcgcctcttc atatgccggc agctttctca cctccgctga
     3721 aaggatcagg atagaaccgt tcctcttcac agcagcactc ttttggagac aggtgttaga
     3781 gagcggcggt gttattatgt tgtagtccta cttgtctgaa acagggaact ccgtcactct
     3841 aagttttgcc gcaccataag gaatcaaagt gagttctgaa agcgcttctt ctgaatggac
     3901 tggactgaga gggggcgggg cggcagcatt gtgctcgatt ttccattcag gcagaagcct
     3961 accttttaca tatgcccgaa ccggcgtgga agccggatcg aaaggattgc aggaaagagg
     4021 tgccgactca aatcggatgg tttccatgaa atcgggcgct aatgcgtaat tccacggact
     4081 gacgggatac agttcaaaat cattgtgcgg tgcttcgccc cggagagaac gccattcggt
     4141 ttccaccaaa agagagtaaa ccagcggacc tctggaaata gcggcgcttt tgttaaaacg
     4201 ctccatgcgc acagctttca tgggaaaccg cagaagaatc acactgttgc aggtccattt
     4261 ttgctgcagt acatggaaag tgccggggac ggccgaaaca ggtgcttcat tgttcagctg
     4321 cactgttgct ccttcagccc aggcggggat gcgcagatgg acagggaact caccggtggc
     4381 atcaaggtga atccggattg tttcttcaaa gggatactct gtttcgatac ggagaaatac
     4441 cggctgcccg ttcagcgtgg tttttaatga acagggcgca taggcaacgg ctgcgattcc
     4501 ctgaggttta tcgttggctg cgggcgtacg catcaaaaga tggctcgtaa atttgggcca
     4561 accctgatgc atattcgaag tgcagcagcc gtaattgggc tcaagaccga agaggttgga
     4621 gtccggcccg ttattggtat agatacggtc gtctgaaacg cggcagataa cctgatttgc
     4681 ctgctgcaca tactgatgtg cccacatgtc cggagaaaag gtgccgggaa gtgcgttaaa
     4741 agcaattcgt tccatacgat ccgcatcttc agcccgaccc agatatgcca gcagcgtttc
     4801 aagagagaag agatattcta cgaccgcgca gagctcagtg ccctgagagg gattctttcc
     4861 tgcatagtgt tcgtctccgg taaaaatgcc ggtaacctgt ccgtgatatt tgtccagtgt
     4921 atccagaagc agctgcaccg cgtgtaaatc gccgggatcc cgggactgcc ggtaccagac
     4981 agccggcgcc ttaacagcca tggcgttatt gactacatgg gtatcaaaaa tacactcgct
     5041 gcctttcatc ttttcagtga atctgaagtt ttcaaaatga tcgcgccagg aaaagccctg
     5101 agaatgagca cgctccgcaa tgttcagaaa atccggattc cctgtccggt caaaaagcca
     5161 gtgcaaagta atcacgaaat ccatccagcg atattttccc caatcgaaaa ggggtttgta
     5221 tatgagaatc ctgtcaatac agcggacatt cgctgtcatg acctcaagga tggaggaatc
     5281 accggtgact tcataatatt ggcgcagtgc ttttaatatg acgaattgcg gccaggtatc
     5341 atgctcctga tagccctcac tttttttggg cccaagccaa ccatctttct cctgatgcgc
     5401 aataatatac cgcatccaga aatgtacggt tttctgcaaa tgcacgtcgg gcagtgtcca
     5461 cgccagcggg atgagaccgt ccaaccaata gggggctcgt tcccagcctt cagcattacc
     5521 tccaatccag ccgctgtgct ggatatccgg ccagaattta tccagattgc cggaaagacc
     5581 gttgctctga atccgcatct gctcttccag ccatccatct gaaatgattt tgcccaaggg
     5641 aaaaggctca agggcgagtg gcgttaatga atgggtatca gtcggatgcg cggcgattaa
     5701 gagactgagg aagaaggaag agagcatagc ggttctccat taaaagtttg ccggtttact
     5761 atgaaagtta tctgaaaatg ccggaggggc acctttgcag aaataatttg ctatactaaa
     5821 cctgaaatgc tatttaccta ttgtaagtgt tgctttccgc acaatcaaga aagggaatac
     5881 aaaacgatgc tcaaggagaa aaagaccatg aagagcaggt gttttatttt cagccttctg
     5941 tttgttttgg ttcagacagg gtcttttgcc cagacagaag gatcagaaag gtacaggctt
     6001 ctaagtgccc gggagtatga agaacgtatg acggcggcgt ggatagggca gatggtggga
     6061 gtaggctggg gtgtacctac cgaatttaag tttaacggtg taatggtgcc tgaaaaagaa
     6121 tttccccgat ggacaccgga aacggttaat cagtttcagg aagatgatct gtatgtggag
     6181 atgacttttc tacacacgct ggaaaaatat gggatagatg cgacaccgag acaggcggga
     6241 atcgattttg ccaacagcgg ttatgaactc tggcatgcca ataaattcgg ccggcagaat
     6301 ctgcgaaaag ggattgcgcc gcctgattca gggcatccgg aatacaacag ccatgcagat
     6361 gacattgatt atcagattga agcggattac agcggcatca ttgcaccggg gatgccgtcc
     6421 atcgtactta aactgggcga tacttttggt cgtatgatga attacggtga cggtctttac
     6481 ggcgggcttt ttattggtgc catgtacagc gaagcgtttt ttgaatcttc cattgaagcc
     6541 attattcaag cagggttaaa agcgattcct gagcagagtc agtaccatga atgcataagt
     6601 gatgtgcttc gctggcataa agaaaatcca gaggactgga caaaaagctg ggagaagatt
     6661 aacgagaaat accatctgaa tcctgcctac cgtaaattct cgtgcgggga tcggggcgct
     6721 tttaacatcg atgcgaaaat taatggtgcc tacgtggtca tgggtctgct ctacggggaa
     6781 ggcaacccgg ataaaaccat tgaaatcgcc accaggtgcg ggcaggactc agattgcaat
     6841 ccctccagcg ccgcggggat tctctttacc acattaggca taaacaatat tcctgaaaag
     6901 tttaccagtg ccttgaaaag ggatgtggca tacagtcata ccccttatac ctttgacggg
     6961 cttctggcta tttgcaatga attgaccaga caggcagttg tttatagtgg cggaaaaatc
     7021 ctgcatgatg cagagggaac tgaatatttc tgtattccag tgcaggaagt ggcagccggt
     7081 gcgcttgaac agtgctggga acccggtcct gtcgctaaca gccgttttac cgaagaggaa
     7141 tacgcgcaga ttctaaccaa agaatgacgt ttcgaatatt ttacagtgac agaatagtgt
     7201 gtaccctgtc gcgaagatag ggtaccactt cctcttcaaa ccaggggttg ctttggaacc
     7261 aggcaatatt gcgtgcggaa gggtggggaa gcggcagaaa tcgggggata tatgatttcc
     7321 agtgcttaac tgtatcggtc aggctggact ttctgcggtt cttcagataa cccttttgtg
     7381 cgtaggttcc tgcaagaatt gtcagctcta ttttggggag caacggtaat acctgaggat
     7441 gccataaggg catgcactcc ggacgtggcg gcagatcgcc cccgcgcgga tgccgtcccg
     7501 ggtagcagaa accggcggac attatggcta tttttcggac attataaaag acgtcacgat
     7561 caattcccat ccagtcgcgc agacggtctc cgctcgggtc atcccaggaa atgccagttt
     7621 catggacacg gcgccccggt gcctgtccta cgataagaat gcgggcttct gaatggaggt
     7681 gaacaacggg gcgcgggccg aggggcaggt gagcggcgca gactgtgcag cggcgtattt
     7741 tttgtaccag aagttccagt tcttcttgcg tgcttaattt ttctgtcaaa gcagagacac
     7801 tctcaaggta ttttatgttt attcacaaga tacacaacac atgtaaaaat ataaacaaaa
     7861 tagacttgtt gaagaatgcc cgatgctcta ctgaaaagaa gaaggctttg cagaaacgca
     7921 acagaaatag ggtttagaag aacaacattt tttaaaaaaa ctatcctgcg caggcctgtt
     7981 gaagaagccc cttggcaggt acttatgact tggtaaaatc atcgccgtgg atgctcatgt
     8041 atttgcgcaa gcgacgcatg gcttcccgtt caatttgcct gatgcgttcc cgggtaagct
     8101 caaagtgttt gcccgtttct tccagcgtgt gggctttgcc gtcaatgagg ccgtagcgat
     8161 aactgatgat agaacgctcc cgctcattga gctgggccat cagggttttc atttcttcat
     8221 cccggaaaaa ctgtaccagc gcctgattcg ctgctgcagg ttcctgaatg gtacaggtgt
     8281 cgcgaccgat cgcactgtcc agtgactgca tattatcaaa gggggaaact gtctgatcaa
     8341 agagctccag tttttttatt tctttaagag tcatacccag agcagcagcc atatcatcac
     8401 tggaaggata agagccgttt tgaatataaa actcttccgc cacccgtttg tatttagcaa
     8461 ggttgtcagt aatataaaca ggaatacgaa ccgttcgccc ctgattggag agtgcacggg
     8521 tcacagcctg ccgtatccac caggtggcat aggttgaaaa tttacagcca tgagaggggt
     8581 caaaccgatc cacagcgcgc atcagtccca gattgccttc ttctatgaga tcaagtaaag
     8641 gcagtccgaa atagaggtat tttttagcaa cagagattac gagccgcaga ttggagacaa
     8701 ttagtttgcg tcgcgcagaa gaatcgcctt cttttgccag cttggcaaga cgaatttctt
     8761 cggaggccgg aagaagcggt atttttgaaa tctcctgcag ataccgcttc aaacttctgt
     8821 cactgggctc aagcatagaa tctcgctcaa tgtagaggac aaaaaaggtt tattgcatca
     8881 gcgaatgata ggctgccatc attttcgcaa acaactcatt caacagaagc acagggcctt
     8941 ctgtacctag aatgaagtaa tgtatcaagg cggcagagaa gactgcagcc aggaaagcca
     9001 gcagtaaaac aaaccagaga ggtatgtgca cagacgggtt gtcacgggcg ctttcctcgt
     9061 ccatgatctc ttccagtacg gcagttgcct cttcatacat taattcggag ctttcccgct
     9121 ctgccgcgat gtccttctct tcgacatccg gcggcacccg gcaagtgtct ataaagttgc
     9181 cttcctccgc tttggcatcc ggaagagaaa cagcaacagc ctgagtttca aaaacttccg
     9241 gattatcaga aaacggcggg gaagattcat ccagggtatc ggaagcacct tgcggtgcct
     9301 tattagtatc tactgtaccg acgggagcca cgaatgtttc atcggaatct ttctgcggcg
     9361 gtggggaaac ggaggcctct gtcttgggaa tttcttgcac ttcttcaggc actgggagca
     9421 cttcaagaag gcgtgcctgg atcatttttg ccttgtcgtg atcgaaaaga gagattaact
     9481 ggtcacagta gtccagtcct tcgtccacca aatccagata ttcacaacag acagccagcg
     9541 gatacaggac gttttttacg ttgggctgcg cctcgttgat gcgtttaagc aagttgcggg
     9601 cggcatcgta atgtcctatc cgtatcagct gaatggcttc ctgataagaa agacgtatat
     9661 cctgaatcag cacaaatggt actcccaaaa aaagaagatt tcttacgtta gggtatgagt
     9721 ttgtcctccg gaaagtcaag ttactggagt tgtttcggaa ctgcttccgt aaaagcctgg
     9781 tcatagtcct tccaaacagg atcataaccg gatttttcta tgaacgtttt tatttctgca
     9841 accgtgcgca catcctcaat ttgaaactgt tcgagggtgg gagatgtggg cgcggcatat
     9901 ccgcccggac gcgtgctgga gcctgcgctc atggcagtaa tccccagagg cagcagttta
     9961 tctctgaaag agggggcctc gcgcgtgcta agataaaagg gcgactccgg gagaaagagg
    10021 cgcagcgcaa gaatcatctg caccatgtta atgtcagaaa cggggaaagg cgggttgaaa
    10081 gcggaaggtg tgtgcttcag tcgtggaaaa gaaagggcta tcgtgctgtt ccagcatcgg
    10141 gtctgcagcc agcgtgcatg caaagcaagc cagaaagcat caacacgcca tttatacaga
    10201 cccagcagaa cgccggcacc cagtcttctt acgcctgcat tcgctgctct ttccagagct
    10261 tccagacgaa aagaaaagtc tcgtttgcgt ccgctgcgat gcatatccag ataggtgggt
    10321 ttgtgatagg tttcctgata cagcgttaca ccgtcaagac cgtgggtgca caggagatgg
    10381 tactcttctt gtgtcatgga atacacttcg accgcaacgg aggaaaacca tcgtcgggcg
    10441 atggtgcatg cctgggctat gtattccggc ggcgccgcat ggacagcatc ccctgtcagc
    10501 agcagcacgc tgctgaaacc ctcctttgcc aaagtgcgac actcgtcttc aatttgagtt
    10561 ggagtaagcg ttgttctcgt ttctttgccg gcattgactg ttgaaaaact gcaatacaga
    10621 caattggagc cacagacatt ggaaaggtaa atgggagcat aaaggcttac ggtgcgcccg
    10681 aactggcgac gagtctgttt gttggcttcc tgtgccatgg cttccagaaa aggaagcgcg
    10741 gcaggcgaca gaagggcaag aaggttttca ggggtgcggc ggtcagcaga cagggcggcc
    10801 tcgacctgtc tttcagaaag cgtggtgtac tgttcctgaa tcagcgtctc gggccactca
    10861 tttaaatagg cttcaaaggt catgctgcac attaatcctg attaaaaaga aaccccgtta
    10921 acggagagga agcggcggca aggctgcgtt gcggtcctaa tccggcgagg taagccattc
    10981 gtcctgcagt agtagccagg gcaaaggctt ttgccatggc tccgtgatcc cgggcatcgg
    11041 agagcgctgt atttacaagc accgcatccg cacctagttc catggcttcc gccgcatggg
    11101 acggtgcgcc cagaccagcg tcaacgacga caggcacatg ggcctgttca ataataatac
    11161 gaatcatttc ttttgtttgc accccacgat tgcttcctat gggagcaccg aggggcatca
    11221 ctgttgccgt accgacttct tcgagacgtt ttgccagaac cgggtctgcc tgaatatagg
    11281 ggagtacaac aaatccttct tttacaagtt gttcggcagc acgaagcgtt tgaataggat
    11341 caggcagcag ataacgcggc tcaggagtca attcaagttt tatccagggt tccagcccgc
    11401 cggcacgggc gaggcgcgcc agtttcaaag cttcttctgc tgtttgcgca ccgctcgtat
    11461 tgggcaaaat cagctgcttt ttcggatcta tggcggaaag aatgctctgg tgttcgggac
    11521 tgtttaagtc aacacgccta agtgctaccg tgacgatttc cgagccgctt gcctcgatgg
    11581 ctgcgcgcaa cgctgccgct gagggaaatt tgcctgtgcc gatcataagc cgtgatgaaa
    11641 aaaggcggcc tgcaatcgta aatgtatcca taggggaatt cgtatccata attaagtgat
    11701 cctgatagat gcagaggacg ggcgggcttt cctgaacgcc tggctgtttt taaatgtaac
    11761 actgcagccg ttatcaatat tcagcgctgc cgaacagcgc cgtgcgctgt tgaaaagaat
    11821 cggagcgagc ctgtttcagg tcatatagta ccaaaataaa taccggactt tgtatttgtt
    11881 cacgacaaaa gaaactcatc tccggcaaaa cgggtcttaa ggcaagagat aaaactgaaa
    11941 gaggtgcctt cttcatgtct tcaattttgc tttgccttgc ggttctttta tcactcaatc
    12001 aaagggatga cttccatgca cctcgtatga accagattca ggtgatcgga acgcacaaca
    12061 gctatcatct ggaaccgccc tccctggtcc tggacacttt gagtactctt gatccccgtg
    12121 tgaaagagtg ggcatattct catgattcac tggacgcaca acttgagcaa ggggttcgca
    12181 gtttcgaact cgatatacac ccgtacgttt caggattcaa ggtgcggcac gtgccgcttg
    12241 tagacgataa cagtacctgt cctgagttta tggagtgttt atccacattg tatttatggt
    12301 cactggaaca taatgaacat gtgcctgtca ccattctcgt cgaaattaaa caagcggagg
    12361 cactgcttgc agccgagccg ctctgtaacg atccggttca aattgtgcag cgcatcgaag
    12421 acgaaatcag aacaatattt ccgtccgaca aactggttac gccttcgtgg gttcaaggaa
    12481 atgcagtttc tctcaggaag aagctggaac tggaaggatg gccgcccctg aaacattgtc
    12541 tggggaaatt tgcctttatc cttcatgacc gcgggaactt gcgggacgca tgcgcttctg
    12601 ccggacaaaa taaagtgctc tttgtaaacg catctcccgc ccgaagtgac ggggcgttca
    12661 ttgtggtgga tgatccctat aatcctgaaa tttctgcgct cctgaaacag aatatgatcg
    12721 tccgggtgcg tgcggacagc gggttaaacg tggatcgccc cgacagcata aagaggaaag
    12781 agcaggcact ggcatgcggt gcccaaattg tcagcactga ttttccgcca ggaaaagcag
    12841 atcctgccac cggatattgt ctttctttgg gagaaagtac ttctgcccgg agcaatcccg
    12901 ttacaggaga tttttctgtc aaggcgtttc ttcaaaccat aattccttag aggaaaaaga
    12961 ggtgttccgg agcgaaccgg aaaagaatgg aactgtttct cttgattaag aatcagattc
    13021 ttgttatatt taccccgaat tcaaagagga atttccctga tgtccgtaga atgctcggga
    13081 gatttgggtg aaaatgtcag aataaaatga agaaaggact tctgtaatgc gtcgctatgt
    13141 atataacctc gatcctcaaa aaatgccgga tgcctggtac aatatcctgc ccgatcttcc
    13201 tacgccccat gcaccggtac tgcatcctgc cacgcataaa cctgtcgtgc ctgaggatct
    13261 cgcacccatt tttccgatga acctgattga acaggaaatg agcagcgagc cggcaatttc
    13321 catacccggc gaagtgatgg atcgttattt gatgttccgc cccacgccgc tgcgtcgcgc
    13381 ctatcggttg gaagaagcac tgcagacgcc cgcaaaaatt tattataaaa atgaaagtgt
    13441 taccccttca ggcagccaca aaataaatac gtccatacct caggcctact acaataaagt
    13501 ggagggggtt cagtcattaa gtaccgagac cggagcaggg cagtggggca cggctctttc
    13561 tattgcctgt gccatgttca acatgcagtg caaagtctat atggtccgtg tcagttttga
    13621 ccagaaaccc taccgtaagg gatttatgga aaccttcggc gcaactgtta ttgcgagtcc
    13681 cagcgataca acagaggtgg gcagaaaaat tcgcgctgcc aatccgggta cctccggcag
    13741 cctgggcatg gcaatctcag aagcagtcga ggtggcggtt accagtggtg gaaccgtgaa
    13801 gtacagcctg ggcagtgtgc tgaatcatgt gtgcatgcac cagacgatta tcggcgagga
    13861 agcgctgctg cagatggaaa tggcaggcga ctatccggac gttgttatcg ggtgctgcgg
    13921 aggcggttcc aattttgccg gtatcgcttt tccttttgta cgggaaaaat tcaacaaaaa
    13981 actggatacg cgctttattg cggcagaacc caccgcgtgc ccgactctga ccaaaggccc
    14041 cttcgcttat gatttcggcg atacaggaca gcttaccccc cttattaagc agtatacact
    14101 gggtcacaac ttcgtaccgc ccggcattca tgccggcggt ctgcggtatc atggcgttgc
    14161 cgccacggtg gcgcatctta aaaaggacga agtgattgaa gccgtcgcgc tgcaccagaa
    14221 cccctgcttc caggcgagcc tcatgtttgc aaaagcagaa gggattattc cagcgcccga
    14281 atccggccat gccgtgcgga gtgccattga tgaagcgatc aaatgccggg aatccggaga
    14341 agcaaaaact attctattca acttgagcgg acatgggcac ttcgacatta ccgcttacga
    14401 ggcgtatttg cgcggtaatc ttcaggatct ggaactggac gaagaaacgc ttgcaaaagg
    14461 aattgccagt attccggaga ttcaagaata aagacctgac ccctgctaac tgctgaagca
    14521 gggttgcaaa atcaccacta tgcggctgca tcagatttca agagatcggg cagccgcata
    14581 ttttttcagc ccgtcctgcc agtcaggcat gtagtccagt ccggagcggg cgagtttgct
    14641 tctgtcgaga agggaatagg ctggtctgcg aaatgcgtct tgaaaattca tccgttgtac
    14701 aggcaaaaga tccgcgtcga ttttgaattg aaaaaagatt tcccgggcaa aatcatacca
    14761 gctgcagaag cccttgctta cggcgtgata gatgcccgaa ggggctgcag tcgccagaat
    14821 gctgatctgt tccgccagat cccgagcaca tgtcgggctg cacatctcat cagttaccac
    14881 atgtacaggg ctaccttctc gcgcccgggc cagcatcgtt tcaacaaaat ttcgcccctt
    14941 ttttccccgg cagggatttt gcccgtacaa ggatgccgtc cgtataatca aatagtctga
    15001 acagttttca ataacatacc gttctccggc agttttgctt tttccataca cattgaccgg
    15061 gcggacagga tcaatttcct gaaaaggccg ggctggcgag aaactgtatc cgccgtacac
    15121 ataatcagtg ctgatataaa tcagacgggc tcccacatgc ctgcagagta cagctgtttc
    15181 caggcaactg agtgcgttga tctggtaagc gagctgaggg tgtgcttcgc agaaggcagg
    15241 ttcatgcagg gcggcagtga gaatgacaca ggaaggctgc acttcagtaa tgcaggagcg
    15301 cagtgcattg taattggtga tatccaattg aaaatctgcg gcttccagat cggcgccgaa
    15361 ggcctcacct ggcggaaatg cgcacagcag ctcgctgcca agctgaccgg cagaacctat
    15421 aatgagggtc cgggacatgg caagtcagat ctttcaaagg atagtaggac tgagagtgta
    15481 tgctaccgga cttaaaaggt gaagcccagc cccagcgtaa gcacatcgac gccacgattg
    15541 tggctgtcca gtccgccatt agacaggtgc tcgtagcgaa gaacggtgtg caagccgttt
    15601 tgaaagccgt agcctacacc aaaggcggaa aggaaattga gattggagcc attgccttca
    15661 atccgccctt tgtgcgccag agcatgggtg tttgcttcaa gatacacgcc ccggtaactg
    15721 tctctttcaa aataaaagcg aagcccgaga ccaagacctc cgccccatac cgtatcgtcc
    15781 cctttgcttt tctgctcata gagaaacaag ggaagaaggc gaagtcccag cgaaaaatgg
    15841 gaactgaagg gcttctcgaa ctccaaagta gacttgagca gaaagtcacc gcttctgtca
    15901 gtgccaccca gttcaagccc cggaccgcct gataattcaa tattgaattt tccaggatgc
    15961 gctccttcgg ccgcacaaag cgatgttgta aagaaaagag aggcaagcag gagaagactg
    16021 acgagaagat ttattttcat tacgggtacc attttgttag aacaaagaaa tatatacgca
    16081 aaactgttac gcggagtata gcatgaaagc cactgaaatt acagttatag actgcttccg
    16141 gctcctaact ggtctatcat ggtgatcatg gggagaaata gggaaacgaa caggatgagc
    16201 accgcaatgc ctacgataat ggtaaagatc ggttggagca cttcgcccag tcccgcaaca
    16261 gcaagcttca cctcttcgtc gtaaatgtcc gcaatctgct cggcgatctg gtcaactttt
    16321 cctgattctt cgccggtgac aaacatatcc gtaacaacgg gcggaatact tttagactgc
    16381 cgcatcggct cttcaagact gccgccctgt tccacagaat ggcgcatggc tttcagtgtt
    16441 tctgctactg cacgattgtg tatggcactg cgtgtcagat caagggtcgc catcatggaa
    16501 agcccgctgc gcagcaggag ccccatggtt ctgcacattt cgacaagtgc gttcttgtgg
    16561 ataatggcac caaggacagg tattttcagt ttataataat ctgcttttat ccggcgttca
    16621 ggagttttgg tgagccagag tttatacaca agtcccaaaa ttaccagaag cacaaaaggc
    16681 agccaccacc agagacgggt cagggcagac agctcaatca gcagtttcgt gctggcaggc
    16741 gtgttcatat cggcgcgttc aaaaaaatct gcgaaaacgg gaacgacaaa agaggtgatg
    16801 acgaagagag cgccagcgca ggcaataatc agcagcaccg gataaatcat ggcgccccgc
    16861 acttttttgc ggagcatctc gcgtccttcc ctgtaggtga ccaaacggcg cagcacggtg
    16921 gttaaagtgc cgcttgcctc gcttgctttg atcagattga caaaaacgga gtcaaaatag
    16981 cgcggatgcc gatcaaaggc ctgccacagc gtattgccac cttccacata cagggcaata
    17041 tcattaatga gccctttaac ggctaaattt tcagcgcggc ttgccagcga ccgtaaagag
    17101 cgcaaaatag gagtacccga ttccaaaagc ataataaact ggcgcaaaaa attggttaca
    17161 tcggcgtagc gtacatttcc cgaaccatga aaaaggcggc gggcgggtgc tgtttgcgca
    17221 tccgtggaac ttcctgtcag agaagcgcca gtctgttcac tttccgagga accgaggtcg
    17281 aagcgtttgg gtcttttggc gaccggtttg ttggcatcag gttcgagcat gcgtttcatt
    17341 tcctctcata tagatcctgc acggcaggca ggcactcact tatcggcagg tacgccagga
    17401 aagcacttgt atttcgtttc tatccagaga cacaacagcc gctattgaat gcctggaaag
    17461 gggataggca tccagagccg catttttcaa aagaacagca ctgctttcga ttttccagac
    17521 aagtccttct cccggaaaat tttcaggaga aaaattaaat tcctgccaat cctcggcaga
    17581 aagggcggac atgctggctg agacttcagc gcgataatta tcaagcagga cagggcggtc
    17641 ggggtttgag ccttgcgtat aaccatatac cgggaaagtg taaattgtgg aggtcacagg
    17701 caacatgcct ttccgcaaag tttcctgcaa ataagcagcc gaactctgaa tacccgcttc
    17761 cgcataaaac tggcttcgaa gtttgcggag gtcaaatgtg cacaaatcgt cttctaccat
    17821 cagaaaatgg acataactgg cgccaagcag cgtgaaaagc gtaagcaggg caatagctgt
    17881 aaaaagagca gcaccgcgcc gtccgtcaca acgctcaggg cagcaaaaga aaagaggcgt
    17941 aaccgccctt ttgattgttc ttttatttga catgtatgtt aaaaaccacc attcgggaaa
    18001 tgtgccgtgt aggatcgcgc ggatcaacac aggtcaaact gatccgtact gcatcgggca
    18061 tttctcttct gttccattga gtgttccatc tttttccatc ggaatacaaa atgcgcattg
    18121 ccaaaatatt ttcgagagag aaagattcct gaggcactgc atcttcgatg tcaagttcag
    18181 aaacataacg gatgaaacgg gcgctttcag tagtccgctc aattttatat ctgacaagaa
    18241 aacgatcgtt acgatcctgg agaggattat agatgcttac cggaaaagaa acactgtcat
    18301 cctcaaaatt gacattccag taccgcctgc tgtcttcgac agatgcccgc ataccctcaa
    18361 ggcccctctg gcagcagtct gtgggcagaa tattttcaaa atctttgcgg aactctgtaa
    18421 aacagcgggc aagcgttttt tcgagagtca gagcggaaac aagatcgttc cactggtaag
    18481 agactttgac aaacccgacg aatcccattg tggatacaac acccagcata ctcatcacca
    18541 gaagcagttc taaaagggta aagcctctat ttggtcggga ataataagtt atattcactg
    18601 tgggcctgcc tctgtttctt ttgcctggtc aaggctttga aagagggaac ggggaacaca
    18661 actcgtcagg gccagcatgc ggggccgacc ggataagcgc cagtgcacag aaagagtgac
    18721 ctcgaggtaa tccgctgttt cggaggggat cctgccccac gcctgccagt aaaaagatct
    18781 gtgcaaagtt gccgttttgt cctgagcttt ttttgtggcg agtattgtgt ccgggagcag
    18841 cgcaccggaa gcggcaagtg tggagttttc atttggaagg attgggaaaa gaccatcttc
    18901 tcccgtttca tcgaaagacc acacatattc ccccgggtga gcacaaagca cggagagctg
    18961 agtttcggca atttcagcgg caacacggtt attgcgtagc tcttttgaac gctgcaggtt
    19021 attgccgaaa agggaaactg aaatagccag agcaacagaa ataaccgcta gcgcggtgat
    19081 taactcaaga agagtaaatc cggctgcagc tttgttgtta taaaaaagag acttgcgaca
    19141 aaccaccgtt caaagcttcc ttgttccctt aacaggcacg ccccgtcatt cagcagctat
    19201 tacgtgttgt tatcataata gaattcaaaa agtaaatcaa gagaaaaaaa aatattataa
    19261 aaataaattt gactttttaa aaatattatg ctataagtgc tttgaagatg gtgagggaca
    19321 ggtgtattcc atttagaaac gcaagaaaaa actccaaaaa gaaagagagg tgagaagtat
    19381 gcattgcgca ttatgtaatg aatttgtcga agacaacgaa cttgcctgcg gtgacgccat
    19441 agaagtggac ggcgagtatt ggcatagcga atgttatgtt gagtactacg gcgaagagct
    19501 cgaagaagcc gtgtagtatt cctgcagccc ttttgttttg aaagcgcctc ggtcttacct
    19561 gtgaccgagg ttttgttttt aacggttgcg cgaaaacggg aacatttctt ttggaaaagg
    19621 aattctttat aagaagcctg agctccgtct ccgaggtctt caaataagtt actgacagtg
    19681 aagggaagta aaaagtactt aaaaagtgaa cgctgtttta ttaaaaattc gtgcctaccc
    19741 gcgcgcctca agaaatagag ttattgtaac tgaatcgggc ttacttcagg tgtatgtgac
    19801 agctgcgccg gaagacaaca aagcaaatac cgccattatt gcaacgctct cgaaactatt
    19861 aaaagtcgca aaaagcaaca ttgaaatcgt tagcggactg caaggacgtg ataaaaaagt
    19921 aaaagtgtcc aatgtatctg aaaaaataat taatcagcgg cttgaattac ttcaagcgga
    19981 taaatcataa tattttctga cgattaaaaa ttttagcagt aggaatcata aatggattta
    20041 atgaaaaaaa ttgagcaggc agcagaggtt gtccaaaaac atttgacctg gcaacctgaa
    20101 gtcgcaatta ttttgggaac cgggttagga tcaacgggga gcagtataga gaatcctgtc
    20161 gttattgact atgccgctat ccccaacttt cccttgtcaa cagccgaagg gcacgccgga
    20221 gaaattattg caggaacgct cgccggcaaa aaggtcatcg gattatccgg tcgtttccat
    20281 ttgtatgaag ggtattccat ggaggaagtt acctttcctg tacgggtggt gaaagcgctg
    20341 ggagcacata cgctgatcgt ctccaatgcg tcgggaggcc tgaacccgca gtaccgggca
    20401 ggcgatcttg tggtaattac cgatcatatt aatttgatgg gaaacaatcc tctcataggt
    20461 cctaacgatg accggcttgg cgagcgaatt ccggacatgt ccgaggcata ttcccgtgct
    20521 cttgtctctc ttgcggagca ggcggcactt gaagagcaaa taaaacttca gcggggcgta
    20581 tatttaggct gcaccgggcc ctgcctcgaa acgccggcag aataccgctt tatgcgcatt
    20641 atcggtgctg atcttgtggg tatgagtact gttcctgaag tcattgtggc agtgcatgcc
    20701 ggacttaaag tgcttggctt ttctgtggta actgacgaat gctttcctga ttgcctgcag
    20761 ccgctaaatc tggaacgaat tgtgcgatgt gctgctgaag cggagcccaa attgacgaag
    20821 cttattaacc ggtgtttgca tttattatga tggtgtttgc atttattact cttttaggga
    20881 actaagaatg cttgtaaaaa ttagtcgcgt cttttttctc ctggcctgca tccttatggg
    20941 gtttatctgg agcaaattca ttctggaaaa tatggtggag tccgggcgcc agattgaaaa
    21001 tgtacagagg tggattgggc tgggtggtct gattggggca gttgttgcca tcgctgtgct
    21061 tttggcattg cactttgtga cgcaggaatt atatgagcgt tttgcccctg cggttgttgc
    21121 catagtgctc gcattgcttg ctgggtactc catttcaaga tatcttcttt tctggtttcc
    21181 ttccattgac atcacttcgc aggtttttat ttctgtaacg gtggtgctgc ttttcggcta
    21241 tgtgggcatt tatctgggat taacccgcgc ttcaaattgg gaatccctga tttccgccgt
    21301 aaaacacagg cagtttcaaa acagcccgac ggtttcactg aagattgttg ataccagcgt
    21361 gcttattgac gggcgcatcg cagacatctg tgaaagcggt tttcttgagg gtacgcttct
    21421 tatcccgcgc tttgttttaa aagaattgca gaatatagcg gattcggcag atgtcgtaag
    21481 acgtgccaaa gggcggcgcg ggctcgatat actgaaaagt cttcaggagt ctacagcaga
    21541 ggtctctgtc agcattattg aggataaccc caacgcaagc aaagaggtgg acggaaaact
    21601 ccttcttttg gcaaagcagt atggcgccaa agttcttacc acggacttta atctgaataa
    21661 agtggcgcag attgacggag tggttgtttt gaatatcaac gatcttgcca atgccttgaa
    21721 gcctgctgta ctgcctgatg aaattatgga agtaaagctt atcaaagaag gaaaagagcc
    21781 ttctcaggca gtcggctatc ttgatgacgg taccatggta gttgtggacg gcggacgcaa
    21841 tcatctcggc aaaaacgtgc aggttcttgt taccagtatt ctgcagactt ctgcgggacg
    21901 catgatcttt acccgctttc acacacaggc ataatgagag ttcaggctgt cataccagct
    21961 gccggtatgg gggaacggct gggcgcatca cgccctaaag cgctggttga aatcaacggt
    22021 gtgccattgc tgcgccacac actgcttcgc ctggcgtctc ttttgacacc gttgccctgc
    22081 atcgtttcgt accccgaagg tttccgaaat gaatttgagc atgcgtgctg cgacttgaac
    22141 gtagtactga tctgcggggg aagagaacgg caggagtccg tttggcttgc cttgaatgtg
    22201 ctggatccgc aggttgaaat tgttctgctc catgatgccg cccgcccttt tccggatcct
    22261 gcttcaatac agtgcgctgt ggaagaaacg aagaagtggg gtgctgctac ccttgcaatt
    22321 cctgtgagcg atacgattct ttcagtttcc gaagacggat ttttaaaaga tacacccgac
    22381 agaagagagc tccgggcatg ccagacaccg cagatcttcc tcaaagaaac cattacggcg
    22441 gcgcaccggg cagcgcatga ggaaggcagg cagtttacag atgatgcgtc catggttcgc
    22501 ttttatggcg gacaggtgaa gattgttcca ggcacggtcg gaaattttaa aattacaact
    22561 cctgccgatc ttgcctatgc cacgtattta gtgcaggagg gaaaagaatg attcgcatcg
    22621 gcatcggtta cgatatccat cgtctggtac ccggacgcaa gctcatactc ggcggtgtat
    22681 gcattcccca tgaaaaagga tttgaagccc attcggacgg agatgttttg tgccatgcaa
    22741 tttccgatgc attgctgggt gctgccgggc tgcctaatat cggtgtcctt ttcccggata
    22801 cagatcctgc ctataaagat gcagacagta taagcctcct taaaaatgtg gtgcgacggc
    22861 tgcacgacag aggttttcgt cctgtgcaga tggattccaa catagtggcg caacgtccaa
    22921 aactgaaaga ctacgttgaa gagatgatcc attcgctttc tgctgcgaca ggaatgcctt
    22981 cagaaaatat ttctattaag ccccgtacga atgagcattt aggggcagaa ggcagggaag
    23041 aaggaatcag tgcacaggtt gtggtggtca ttgaaaaaat tccacctgaa gaagcctagt
    23101 tttattcagg aagaatgtgc acaacacgac cgtcaaccga aagccgtccg gcggcatata
    23161 gggcgatggc ttttggatac aactcacgtt caagcgcctg caccctgtca gcgagtgatt
    23221 ctaccgtgtc ctccggtgtc acagggacac tatcctgaag aataatcgga ccgtgatcat
    23281 agagcgaatc aacaaaatgt actgtcgctc cagttatttt ggctccatat gcgagcacgg
    23341 ctttgtgcac gtgattgccg tacattttat gtccgcaaaa agcagggatc agtgcgggat
    23401 gaacgttcat gatacggttg gcgaaggcag ggggcacttc tagaaggtgc atgaagccgg
    23461 cgagtacaat aaggtcaacc tcatggcggc ctaaatgctc ccagatagct gcattgaagt
    23521 ctttctgggt gctgtaatcg gtacggggga ccgcatagga cggtatggct gccttatcgg
    23581 cgcgcacaag gccgtaggca tcttttctgg aagcaatgac acaggaaatt tgtgcgtcca
    23641 gagtcttccc tgttattctg tctattagat tctggagggt gctgccgctt cccgacagca
    23701 aaacagccaa acgaatcgtc atgataaacc cttaatatgg caaagatgat ggttgacggt
    23761 gcctgatata tggcattata tacctttgtc aaggggcaca tccatagaaa cgaaacagat
    23821 tcattgttgc aggcaataaa aacaaggaat gtactatgaa gcgtttttta tttttcgtta
    23881 tgatcgtgac agcgctgact ttaagcggat gcgaaacgct tgaaggaacg gcaggctccg
    23941 gcttttttaa tcagataaat ctcttgagca ctcagcaaga agtgtcgttg ggtcagcagt
    24001 tttctgttca gatagaagaa tctgaaaaag tccttaatca ccctgcaata cagaattatg
    24061 tgtcagaaat cggaaaaaaa cttattgctg tggcgccccg acaggacgta ccctattctt
    24121 ttaaagtcat agacgccccc gacactgtga atgcctttgc ccttcccggg ggctatatgt
    24181 atatttacac gggtctctta aaaacatgct ccaatgaagc ggagcttgct gctgttatgg
    24241 cgcacgaaat cgggcacgtt gctgcacggc atcatgggga aatgatgacc cgtcagatgg
    24301 gcatggagat ggttaccgac atggtgctgg ggaaaaagcc gggtatggtc gcctctctgg
    24361 cagggcagct gtttactgcc ggtgtgtccg cccgttacag cagagcacag gaaagagagg
    24421 cggacagcct gggtatggag atcctgtatc gtgccggcta cccgcctcag ggaatgattt
    24481 catttatgag caaacttatg gaagcagacc ggcgccaggg tggtggcaga agtatgccta
    24541 ttttcgccac gcatcccagt cctgaggaaa gagtggcgct tttgcaggag ctgcttcaga
    24601 gatacccggc aacgccgcag cacaatgcca actacggcga agaccggtat cgccaggcaa
    24661 tactgtcaca attataaaca acactagcga tactggcgag cccaggcctc aagggcgttt
    24721 tgctcacagc ccatacgcca gcgcatgagt cgctggcagc aatccgcaac gatgcccgag
    24781 caggcgtcct gttgtgctaa attctcaaac tcacccgggt cattctgcag atcgaaaagc
    24841 tggggaggca tggatgcttc gccgaactgc acatacttgt acttgtgatc ccgaagcatc
    24901 cataaccggc acgcctccgg attcagatta gcagtctctt tcagaatgtt gtaatagtaa
    24961 aattcgaaaa taattgtact tttatggttc cggcagtttg gatcggtcag aaggtatctg
    25021 ggatctttcc cctgaaagcc ggaagagggg ggagcaccca aaaaattaca gatcagaggt
    25081 gcaatatcca cgttttcaat gagcacgtcg ttgagcgtgc cccgtgtgct gtccgcttct
    25141 gcagcgggat ctcttataag cagtggaacc cggacggcgg cgtcaaaata atgcgccttt
    25201 cctgccagat agtggtcacc cagataagtg ccgtggtcgc tgttaaaaat aattgctgta
    25261 tgctcccact gccctgattc cttaaggaaa tcaaagagcc ttcccagatt gtagtccaac
    25321 tcagaaatca ttccgtagta acaagcgcga acgtcgcgcc agctgccctc gtccagaaat
    25381 tccgtctgcg cccagtcggc gcggcaccgg gagaaatacg ggtggtcatt tttggcttca
    25441 cgagggtggc ggaccggtgc gggcatttcc ttcggatcat acatggagtg aaagggtgcg
    25501 gaacaaaggt agggaccgtg aggcttgata taattcaggg aaagaaacca ctgtttgtcc
    25561 gcattcccgg caatgtactc catgcttttg tttgtgacaa aagccatttc actgtgctca
    25621 cgtttgtatc gggcaggata aaaacagggc aggtgcgccc ctgccccggt ttccggtatg
    25681 catggagagt acatggtctc gtgcgtgcag tgctgttccg gatacccttg tgttttcagc
    25741 cagtcgtacc attccggact gtcatattca tgtttcagca ccacttcata cccgggcaga
    25801 aaatgttcat aactgagcga gtgtttgtgt gggtgtcctt cgggaagcag agacgggtcg
    25861 acggagtaat cgttatagcc gacgatagcc ggcgacagac cgaagcgctg cagatggacg
    25921 gcaaggttgt gctcagcatt tttaagaggg gtaagattgt ccacgacccc ggtagaacag
    25981 gcatatctgc cggtatgcat ggacatgcgg ctgggagcgc aggggctgct ctgacagaag
    26041 gcgtttctga aataaacccc ttcagcagcg agggcatcaa gattcggcgt ttgcacaaag
    26101 ggatgaccgg cggcacccag acattcaggt ctgaattcat cgctgcatat ccacaataca
    26161 ttcagttttt gtctgtcagt catggcggca ccctgtaaag caaaagcagg cagaaattga
    26221 agaacaatca aataatatgc tgtaaccgtt cagccagacg tgtatagcgc cgggtgatag
    26281 tgttgttgtt gactgcaaga gaaacggctt tggatgatcc gactaggatc actttttgcc
    26341 tgccccgcgt aattgccgta tacagaacat tgcgttgaag catcatatag tgctggggaa
    26401 gcaggcacag cactaccgcc ggatattcac tgccctggga cttatgcacc gttgtgcaat
    26461 acgcacttgc cagctgatgg gcctgatcgg ttgaataaat gacccgccgt tcatcttcga
    26521 agcaaacttc aagttccttc gcttcctgat ccacctgagt taccagccca acatcaccgt
    26581 tgtacacgtc cagatcataa ttattatgca tttgcatcac tttgtccccg acggaaaaac
    26641 ccgccaaatc aacggtgttc tgctgtgaaa tcagcgcagc acgcaaggcg gcattgatgg
    26701 catccacgcc tgcagagcct cttcgcatgg gagtcatgat ctgaatatct ttgagcgggt
    26761 gcagatggaa gctggaaggc agccggcgtg tgactatatc gactatggtg cttaccgcct
    26821 cctctggttt ttctcgttca atcataaaaa aatcatgctg attgaaaaga ggagtctctc
    26881 catgattaat gcggtgagca ttggtgacaa tggcactttc ttctttttgt ctgaaaatag
    26941 tattcagacg aactaccgga accctgcctg agtcgataat gtcccgcaac acagaaccag
    27001 gcccgaccga ggggagctga tcggaatcgc cgacaagaac cagtctgcag tgaaggggca
    27061 aagcattaaa cagcgcaaaa gcaaggtgta tatcgagcat gctcgcttca tccacaacca
    27121 gtaaatccgt ttcgatggga ttatgttcat ttcgtgtgaa tgaaccggtg agcgggctga
    27181 attccaggag gcggtgaatg gttctcgctt cctgacctgt tgcctgttcc attcttttag
    27241 cagcgcgccc cgtaggcgcc acaagaagag acgagagtcc ttttgcgttc agaatttcaa
    27301 ggacgctttt tatgatggtt gtttttcctg tgcccggccc gccggtgatg accatgacct
    27361 tggaagagat ggctttttct acagccctgc gctgttctgc cgccagagag atgcgaaaac
    27421 ggttttctac ccactgaaac gccttatccg tctctatctt gatcgcttcc agcggtgctc
    27481 cggcaatttt gtcaagccgt gctgcgcagc ctttttccgc tgcatacaaa agcggcaggt
    27541 aacaggcatc attgtcacag acaatttttc ttttctctat cagaccggaa aagggttcta
    27601 tcagcgtgtc tgcggaaagt ttaagaagtt ctccggtaca ttctctcaat tgatcgattg
    27661 gaaaaaaaac atgccctttt tcagactgtt cctccagcac gaacagaaat cctgcttcta
    27721 tgcgaaaagc ggagtctttg gcaatcccca tctgatgcgc tattttatct gcagtaagaa
    27781 atccgacacc ggtgagttct gtcgaaagct tataaggatt ggcacgcagc tgtgccgctg
    27841 cagattcgcc gtactgttta taaatgcgga tagagagacc ctgcggaata ttatagcttt
    27901 gcaggaaaag catcacagag cgggcggcat tttgtgctgc ccaggctttt tttatttgtc
    27961 cggcacgctt tttcccgatg cccggcacct ctttcagtcg ctccgggctg tccgcaatta
    28021 ttttaaggct ttgcaggccg aagtggtcta caattcgctt ggcataaaca ggaccgatgc
    28081 cgtcaatgag cccggagccc agataccgtt gaatcccttc ccgggacgta ggctgtatga
    28141 tttcgtaaga agaaagttta aattgcctgc cgaacttgag attatcgacc cagtgccccc
    28201 gcaggcgcac ggtttcccct gctgaaacgg ctgccagatt tccaataaaa gtaagtgccg
    28261 aaccgtcacc ggttagcagt ctgccgacta aaaagccgct ttccacgttt tcataaacaa
    28321 tcttatcaat cacgccttcc acagagatag ccccgccagg atgagcagac atctcctgac
    28381 cggcatgggt gctttctacg gaagaggaat ctgcgtggat ggaatctgtc atgcaagagg
    28441 ctcactgtca aaacggcgca tggtcacaaa gtcaatggct gtacgaggac ttaatgaaac
    28501 taagtatagc actgtctgtg caacatcttc aggtgtcatc cagccggttt tgtcccgatg
    28561 cggcattgca tcccgggaca tctgcgtatc cacgcctccc ggacaaacag catggacagc
    28621 aatattgtaa gggcgcaatt ccagtgcaag acttttcgtg aaaccgttga gcccatgttt
    28681 ggctgcacag taagcagcct gcccgtcaat ggcttttaat cctgtaacgc ttgaaatgtt
    28741 gataatgcgt ccctgtcggc gtgtcatcat ttgaggaaga aaagcgcgcg tcagcagaaa
    28801 agcagcggtc agatttatag tgagagaatg aagccagtct tcagcactta attcccatac
    28861 aggcttgaag actgcagatc cggcattgtt tacaagcaga tccagaggag aaaatttctc
    28921 ctgtacagcg ttggcaagct gcttgatggc gccttgttca gtaagatcgc agggaaagac
    28981 ctcggcaata ccgcccttcg accggatagc cgctgcagca tcggccagtt cactttccgt
    29041 ccgtgccgtc aaagcaattt cataaccgat acctgccagg gcaagggcta cagctcttcc
    29101 tatgcctctg cccgccccgg taaccaatgc tgttttccgc tgtgtcatgg aatggcggaa
    29161 ctccctccgg cagaaaaatc tttcttatcc agaatctgat ctgacagact ctgaattgat
    29221 tgggtaagca cttggggatc tgccgtgtcc aaaccttcat aaacgacttt gtagtccgct
    29281 tcttcacgga gaagcgaagg atctctgcca gtattacgga tggcattgac aacttcaagt
    29341 acggaaatac tgtgtgcgga acgtgcgagg tgaaaactgc ttggaatcga atccgtggcg
    29401 ataaccatcc tgttatcttt gaggatatcc aaagtttcct gcagcaatct tgtaggcaca
    29461 ttccacaatt ccgcagcttc cttgatttga aggggctcga gactgttttt aaaccgaaaa
    29521 ctgagttcca gcatcgtgcg gacagccaga gattctttgt atgccgttgt ggtttccgcg
    29581 gcaagccgtt ccagagcaaa tgtcttttca ttctgatagg cgaaagaaac tagagcgccg
    29641 aaaagaagaa gaagccagct gaaataaata tacatcagca gcacaggaaa cagggcaaag
    29701 gcaaaaaaga aatttgtgta gttgatgagc ccgtattgaa atttaatgta gatccaggag
    29761 ttgagcatgt agagactggc ggcgaccaat ccgccgatca gcgcataccg aaactgaacc
    29821 ttggtgttgg ggacaaagaa ataaaccagc gtaaaagaaa gactcagtac gaggaactgg
    29881 gcgctccgca gcaataacgc tgcaggcccc agtatttcca gtacatagga gttttccagt
    29941 gcggcgctga tacccatgat gaaggcggca acaaaaggca gcatgaaagt aaggagcata
    30001 taatgcccta tcacgcgcaa gggatttctc tgcctgctaa ccccccatat tctgttgaag
    30061 gtatattcaa cgttccgcat gaaaccgaag acagtagtga ggatgaagag gagccccgat
    30121 acacccagcg ctcttggact tcgagccgct tcatcggcaa aacttacaag atattttacc
    30181 gggttctgat acagagggtc attggtgata ccgtctttct ctgcaaggat agggaagagc
    30241 gtagtaatta aatgccgttt taattgttcg tcgcttccgg aaaagttttc gggatgcacg
    30301 ccaaggacag cggtattgtc atctgcattc tcatcggcga cagggaaaag ctcatctaat
    30361 tcttcctgtc ttttcgattc ttcttctcct tcttcttccg tatcgcctat gtggcgcaaa
    30421 gcaacgacgg ctttggacag cctttcatcc attttttcat agatctgatc gccgaggttg
    30481 aaagtttgta taaagtaaaa catcagggcg agaaagggta ctagaaaaag aagggtggca
    30541 aaggtgagag cggaggcacg caggaggagt gtttcttcca tcaacccacg cacaagaaga
    30601 atggcggcac ggatctgttt tatgatcagc ccgctgggca ctttttcgcc gggcctcccg
    30661 attctccaga tgtcatgtgt aataaactgc ctgccccgct ggaatgcggc ggaaatttta
    30721 tgaagtgttt caagcaattg tgacaacatg acatttctca cgggcgaatc ggctgcagat
    30781 tactctgttc ggttacgaag tatttgcgtt actctaaaat ttttatacga aggtctttga
    30841 actggatttt cataggtggt ccgggatgca tttgtaaggc tatatgccct ttttgccggg
    30901 aaaaaacctt gtcatgatct tcaaccgaac acatgatctg tccattaatg gaaagggtga
    30961 tggaagcgcc tcttgcacaa atttcatacg tgttccagac atcctgatgg actgcttcca
    31021 gaagtttggc aggatcatcc agcaccgttt cagttttcct gccctcttcg tctatatatg
    31081 acttgaaacc gcgcataacg actccgccac ggtcatgctg aaagaggcaa cctgtccatt
    31141 ctttcccttt ttcaatgtct gcctggtagc cccatatatc ccaggcagga cgggcctcac
    31201 tgcggaaatg gacaccggaa ttgccacctt caccctgaat tttataagca aagcggagaa
    31261 taaaatcctg aggctcaccg cccttccaat aaaggtaatg tgccgcaggg caggggtgtt
    31321 ccggtgtgct ttctgcggta atggcgccgt cctcaacacg ccactcaccg tctttccctt
    31381 cccaaccgtc aagagacgcg ccgtcaaata tggatacaaa gcccgtttct tccgctgttg
    31441 acgaaaaggc aaggatgcag aaggcaagga ccggtaaaac catataaaaa acttgtttca
    31501 tactctgtct ttcgtgtcct atcatattca gattgttggc aattcgtaag ctgggcgtct
    31561 ctcccgggcg agaaaagcat ttgcctcatc gcaatccgta aatttttccg tttcggggtc
    31621 ccaatgaagc ggcttattga cgaggccgat ttttctgacg atgttaatga ggtagcaaag
    31681 ggtcgtgctg cgctgcccga tttccacgtc ggcattacat ttttctcggg tcttcatgca
    31741 ctgcagccag ttttcgatgt gaggctgtgt ctcaggtaca gaaagaggac ccggattgga
    31801 agggtcagcg accagatcct gccgggtgct gtagatccgg tctcgattga tttctataat
    31861 gccgtcttcg ccttcaaaaa ccgctccaag accggggccg ttgttgccgt cgaaggtaaa
    31921 gtacacgggg gtgccgttag caaaacgcat ggagacacgc gcacgggggc cggttgtgtt
    31981 caataccatt ccataataag gcgcaccggt ttcattttct ccgggctgac gggttttatc
    32041 agggcaggca gggcgatcct gtacctcttc ttccaaagta atttctaccg gaccggtaag
    32101 atccgttccc agacctcttt gaagctgatc atagctatgc gtgccccagc cggtgacacc
    32161 gaaagaaata ccaccaccgt cataggcatc ccattgtgcc cagccgcggt gcagttcact
    32221 gtgatagggg agaagtggcg tctggttcag ccacacatcc caccaatccg ctcttccgcc
    32281 atcaggcagg gactctgccg gtttgggctg ccaggtcagc ggactgataa agtcgggaac
    32341 gtgtgccgct ttcagtttgc cgatggcacc gttttttaca agatcgctcg cccagttatt
    32401 taagggcatg gagcgctgct gtgtgccgac ctgtgtcacg catttataat gtcgtgcaag
    32461 tttcaccatt tctctgcctt ctgcaatggt gaggcacatg ggcttttcaa tatacacgtc
    32521 cagcccggca gccatggcaa ggcagctgac ccaggcgcgg gcgtgcgtag tcgttattgc
    32581 cataattcca tcgaggcgtt ctttttccag catctcctga tagtcaaggt agccggcgca
    32641 agtaatgtct ttatccgcca gtgctttcag ggaggaattc aggcggggag caaagcagtc
    32701 gcacacagca gcaacacgca gttccggaat acccagacaa gtcgccacta tttcctgagc
    32761 gcgcccgccc agacccacga cggcgacgtt aatttgactg ttgggccccg gtgtgctttg
    32821 tccccgaact ctggaagaaa gaaccagagg ggcaacagca gtggttttca aaaagtttct
    32881 gcgtgaaatc ataacggctc ctttgtttag agtgtaatct aaattatttc aacggtttat
    32941 gatattttca gcctttactg cacagggaat caacttggag agcgtcggta aaaaaaatat
    33001 caatctcctg gcgcgagagg aatacagcca cgacccaaaa atccgagcgc actctctaaa
    33061 gcattctgaa acgcttccag tttgtccggg ttggcttttc tgtcgaaggc agacgtgttc
    33121 gctggcggct tgtcggaggc ggacggtcgc tgttcctccg taaaaggcaa caatcctgcg
    33181 ctgccgggag ctgtataggg aagcggggaa ttcttttgaa actgctccag agccggactg
    33241 gaaagagaag actgagcagc cgcctggtgt tcacttgtaa acagatcaag aaatccgagc
    33301 ccgtaccgca tgcatgccgg tatgagaatg tctccgtgat attttgacag atgtttttcc
    33361 attggtgcac catccgcata tttcggggga taacaatgtt cggcaaagaa tccccgttgc
    33421 agaaacacaa aacgcccggc aatcgttgct acaccttccg gagcattctt tttgcaaagc
    33481 cacttatgtc cgtcaatgga tttgcagccg ttgttggcgc agggtcgaaa agtatccaga
    33541 agaaaacact tgttccagta aaaagccttt cctttgcgat cttttatttt gaaatagcag
    33601 ccatgccagg aggtgcacaa ctgaatgctt ccattaaagg cggttctgag ggcattgtcc
    33661 gactgttcag tgcttccatc cgaggattga agcggcggta tttcgggagg cgctgcattg
    33721 tcggaaaatt gaagggcgcc ccaataactg ctggcataat acgcatgcca ggcaaggctt
    33781 tttatgaagc cctcgacact tacaggcaga gcactgattc gggcaaggag gcgcgcttcc
    33841 gtgaaggcgt caagatgcca gtcttcggta agggcacatt ggtcgggcag gaggctttca
    33901 gcaggtgcag caaccaggag gtctgttact tgttcctgaa aatctgtcca ataagcctca
    33961 taaagactga tattaaaagg atgccatatg gaatgaacat gacaggggct tgtcatatcc
    34021 tgcagcaggt gaaaaattct gcctgcagaa gcccacaata ggggcccact ctgcccctgc
    34081 atctctttgg caatgccttt tccgtattca agcgccgagc cgccaaaacc gtgcttccag
    34141 agaaaacctc ttccggtgag ggggttgaaa gcgtgggtga gaggcgtcgc tcctttatcc
    34201 tcttccagat tcgcacgcag aaaagcagcg tgctcaattt cgtcgggagc cttagcggga
    34261 tttaaatcgg agtatgtgtt gagaagatag tcagaaaaag cgtagtgcac cggttcattg
    34321 tgttttgcgg tgagggtggt ttccgatgca ggggcgtcta aagctacaga ccgagattgt
    34381 aatgccgcaa tccattactg ccacaaaggt ggaaaccacg aaatgaagcg gtacagcatg
    34441 ggctcaacag aaaacatatc catcagttta tcgtacgccc agtgttgatc cgcgatagaa
    34501 agcgaagctg ctttatccgc caggagccgt atcagcgctt cgtctttggc ggacattttc
    34561 gtttccagga actgtatttc gctggaaata acggagagag ccgcctcttc agtttcctct
    34621 tccgctttag ttttcaggta agccagtgct tccttcgttc cggctgaacc gacagcttcg
    34681 taaaattcgc atcgcaccga aggagattcc gtctttccca atgtgtcaaa agagctgaag
    34741 gggaagtcgt tagtgtctga cagagaaagc ctcagcgcat aaatacaggt taatgggtca
    34801 ggatgggtca gcagcgtgga aagagaagat gtgttttcag aaactgggga gccagccaag
    34861 gcgatgagcg cacttctgga aaggaggacg ttttcagtcg caagtgctgt ttgaaaaagg
    34921 gcgtgaacgg tttctgtacc cagtgtgaaa tgtttcagtc catagcaggc agccatgcgc
    34981 agcgcagcgt cattttcttc gtttaatgcc atgttttcca gcactgactg catttcttgc
    35041 agagggaaat cctgcatgag ccttaatgcc tgaaattgct gctctgtgtc tccggaggca
    35101 agggcatctt ggacgaaagc aactgtttcc gctctgttct gtgcaaaggc ccgtaattga
    35161 tcagcattac aggggatagc gtctgaaaaa aaagcatcgc gactttgctg gaggggtgtg
    35221 cgttttggca aagcacgaac ctggctttgt tctgaagcgt tatgtacaaa gacaggtgtg
    35281 tcagtgatgt ctggtttttg tgacagggat gaacctgcgg aaaagtgatc cggagaaaaa
    35341 gcgatgatag cgggcacaca tctttcctct gtatcggaaa gaagaaggaa tgttgcaaaa
    35401 agaatacaaa gcaaaaaaga gaaggacttg actatgcgca tcgtagtgac cctgacaaag
    35461 aagtcggctt aaaagagcct gttaaagtga acgccgtcaa aaaaaaacgc atccgaaact
    35521 tggaaagaaa aacaaacaga agatccggaa gaataattgc acgatgaggg cactctggca
    35581 cggatcatct gttttcccgt atcaagatgg agaatagatt cggggccgag catttcgacc
    35641 agatctatgg tgccttgtac aaagcattct ttttctgcag tcgcctctgt ggggacatgt
    35701 aagtgctcag gacgtatgcc cagcagagcg gaatttgaag ctgaagagaa aaacgcccct
    35761 ttggcacttt ccggcaagtg aatattattt aaaaaatgat cgcttttaaa aacgagggcg
    35821 ctcccggaaa tttccagagt gccggggaaa aagttgattg ccggagagcc gatgaattcc
    35881 gccacaaatc gattcgccgg ctgctgataa atggtcaagg gcgtgtcatc ctgaagaatt
    35941 tttccctgat gcatcaccac tattcggtcg cccatggtca tggcctctgt ctggtcatgg
    36001 gtaacatata ccatggtgga tttgaggcgg ccatggagac gcatgatttc ggttcgcatt
    36061 tgaaccctca gtttcgcatc caggttggaa agaggctcgt caaagagaaa tactttggga
    36121 ttccggacaa gggcacgacc caaggcaacc cgctgccgct gtcctcctga taatgccgcc
    36181 ggtttgcgat ccagaacgtc actgatgccg aggatggatg ccgcctcacg aacccgctca
    36241 tcgatgatat ttttcggaat actgcgcatg gtaagaccga aagccagatt tttgtaaacg
    36301 ctcatatgag gatatagagc gtagttctgg aaaaccatgg caatatcccg ggattttgga
    36361 ggaactccgt tcatgcgcgc accgtcgatg aatatatcgc ctgaggtgct ttcttccaga
    36421 cccgcaataa gccggagcag ggttgatttc ccgcagcccg atggacccac cagaacaaca
    36481 aacgcaccgt cctctatatg aagagtgcat ggctgcagcg cctgaatgtt gccggcgaag
    36541 gatttgctga gattatgtag agaaacctgt gccatatcga ttccgaaact cgtgtctcag
    36601 gggtttaaaa tgcacttacg gaatcataac acagcaaaag gggctaaagg ctacttttaa
    36661 gatcttatcc gcttcttttt tcggtcagca ttagcgccat gtttttaagc tgtcttatat
    36721 cgattttgcc tgttcccaat acaggaattt cagcaatctg gtagtaatca ccggcacggg
    36781 gacgccacag attcggcagt gtactcccgt tgatccgttc tttcagaaga gcaatctgct
    36841 cttcatcgag cgtatgcagt acaaccagtt tctcgccacg gccttcgtcc gggactcctg
    36901 taacggcgat cgcctgttcg gtaagattta acagctgatg caaagattcc tcaatgcgga
    36961 tatgagggat catttcaccg ccaattttac tgaatcgtgc caggcggtcg gtgataaaaa
    37021 taaagccttc ttcatcaatg gaggcgatat cgccggttct gtaccatcca tcctgtattg
    37081 ccgcctgtgt ccgtttttca tcatgcagat agcccagcat aacattagct cctttaacca
    37141 gcagcattcc ggcttcaccg ggaggcagtt cttctcctgt gtccggatct acaaccatga
    37201 cctgctgata ggggaatgct tttccgatag agccgcgcct gctgtgttca cagtaaaacc
    37261 ccggtgactc ttcagcgggc atattgacag aaacaacagg ggcgcactct gttgtgccat
    37321 atccttcctg aggttctata gaaaattttt ccagaaaggc tttttggatc ctttcaggaa
    37381 gtttttccgc accggcaatc acgcaccgca gacttgaaag ctgttcgggc tcgcagcgcc
    37441 ggataaatcc ctgcagaaag gtgggcgttg caatcataaa ggtacccttg tatttctgta
    37501 ccagttgacc aattgccttc ggttcgagag ggttgggatg cagcactccc tgaatgcctt
    37561 ccagtaatgg cgtccaaagg gcaacggcat agccgaaaga atgaaagaat ggaagaaatc
    37621 ctacaaggca ggtgtgctta tcatgaggca ccatttcgcg caacccgtgc atcatcgtaa
    37681 tgatattacg gtgggttagc atcactcctt ttgggatgcc ttcactgcca ctggaaaaaa
    37741 taatggtagc aatatcgtgt tcgctgcacg gtgctgctcc gagaaaacgg gtaaggagcg
    37801 gtcgcggcat cagcagcgcc agaagcatgg caatgatgcg atcggacgca gtgaccgtgt
    37861 ctttgatgtc atccagatag accggcacgc ccggcggggt gacgggaaca cgggaaagaa
    37921 acgctttcgc agtgacggta tgcgtgatgt cgcagcgctg cgcgcaatcg gcgataattt
    37981 ccgaagaagc tgtgtaattg agattgaccg ggattttacc cattacctga agcgcaatat
    38041 ttgccagagc accggcgacg gtggaaggca tcaatacccc aattgttttc cggctcccgc
    38101 ataatggttt aagcttgcgt gccaaaacga tcacaccaac aagtgcttta aaataactga
    38161 tttgcccgga taactgatct gcataacagg gtcgccaggg ataacgccgt gcgaaacgga
    38221 tgaaaccgta ctgcaggaca gagtcccgga accggcgcgg actaaagccc tcggctgtcg
    38281 ccttttgaac gaaatagcgt tttttcacag cagtgatgcc ttccggcatc gcttcgccta
    38341 cattcaggta aatgggactg cgaaattcag aaaagaacaa acgcactttc ctgttttcat
    38401 aggcatagag aatttcgcag atgcgaccta aggccagggg aattgttggt gcgccggttc
    38461 cttccgtaat gattgaataa tcagaaaacc agggaaggga aactccgtcg ggggcaaggg
    38521 acctggcgcg gtttacacat accagatttc cttgtgccag aagtgtacgc atcttcacta
    38581 cagcggctgc catatcgtct tcagcgtgcg gatcgacggg gattaagtac atggaacggg
    38641 acaggcgtct gatccacggt acttccagcg catctttgcc gattacaaaa taaatctcac
    38701 ggtcgagagc ggcctgaata gccatcagat ctataaaact ctcatgggtc gatagaagca
    38761 gtgcgccctg gtcctcagga atatgcgaac gccctgtaac atgcaggcgt accagagtgc
    38821 catccagaca ccaccaaagc atccgcagcg ccattacggg cgatctccag cactgcagca
    38881 ggccgatgcc gagggcgctg acacccataa aaataaaaat atggtaagcc gtcaacccaa
    38941 aaagattcag caataaaaat acgacagatg aaagggcaat gccaatccag gtaagcatat
    39001 tgacagcggc aattacgccg cctttcatac cgtgaggggc gcgctgctga atagccgctg
    39061 caagcggtac gccgtagagg ccggcaaaaa tgcctagaaa aagaaagagc agcattacgg
    39121 tcaggtagtg accgcccaaa gaatgcagag tgccgattac ggggcagctg cccccgggct
    39181 tcataaaggc ggcaaagcga atcagcggct ccgcgacgac ggttcggtga acgctgtagg
    39241 gtaacgaaag aagcagggag aagaacatcg tgcctaaggc acctatcgga ataagcccaa
    39301 tttcgacctt gccccgcgaa agatatccgg cagccagagc accgatccct atgccgatgg
    39361 caatggctgc cagaagatag gaaattttat cttcacttat aagaagatct gtttgcgaaa
    39421 acttaatcaa gttctgccgt gccagagcgc cgataaacca aaagtaggta tagccgacca
    39481 ctgcataaaa aaggattttg tctttgcgga tggctttgaa atatttgagc atgccttgcc
    39541 agggaagaaa gggattctta ggaatgggct gggcagggtt ggcagcaggc gggcgtgtaa
    39601 tgaaatagga aaaacaggta ccggctatag acaggaatac gaggagtaca gacacaaggt
    39661 agactttgcc ttttgtcagc aaaaacaggg gaccggcaag accggtaccc ataatgatgg
    39721 caagcatcgt gcccatctgg attacgccat tgccccagga aaggcgttct gctggcagga
    39781 tttccggaag aatgccatat tttgccgggc cgaagagagc gctttgtgtc gccatcataa
    39841 aaagaaggaa ccagattaag ggtgtccagc cgagcaaaaa agcgatacca ccggcgagca
    39901 ttataaaaat ttcaagccat ttcgctgcta cagcaacacg ttgtttgctg aaacgatcgg
    39961 caagagcgcc aaatagagcc gggaaaatga taaaaggcaa tgaaaaaagg aatgtagcaa
    40021 aggcaggtac aaaggtatcg gcggagaatt tgccgaaaaa cagactgctg tccgtgccgg
    40081 ggcctgatgg ctgcaacaga gcgagcagat agaaggtaat aacccactga taaacattgt
    40141 cattaaaagc accctgaaac tgggttgcca taagtgccca gaaacctttg ggttggaagg
    40201 tcttttcggg ggcgggtgtg ctcaatttgt atgccataac ggcagcctct cgtacctacg
    40261 gctcatagaa tgagcccggt gcttgcagaa aaagcggaat tagaaaattg acgcctctgc
    40321 ctttgtccat aaatgccgag gattctccga acagaaactc agcattgcga ccagaagaaa
    40381 gacttttgtg aaaaaaaacg cggatgaaat tgtcaggtgg aagaaacgcg gatttcttcc
    40441 agaagatcta taagtctgcg gcattgttct tcatcaaaat cacgaaggac tttatgaatt
    40501 tcagcgcggt aaacaggctc aacctcattg atcagcctcc tgccttttgt cgtaagtgat
    40561 acatgataaa tacgcctgtt cccggagact tcattgcggc gaaccagatc tttgctttca
    40621 agtttatcca gaaccgaagt tatacttgcc cgggttacta cgagtcgtct gcccaattcc
    40681 gattgcgtcc agtctttctt tttgtatttc agggcaaaaa gaacattaaa ctgtgcttcg
    40741 gtaaggccaa actgacggaa taaagtggcg ccccgtgttg caatcgtcgt ggcagtacgc
    40801 accacattga gcacaagctc atgtttaata tcctcaatag gggtttccag ttccaattct
    40861 ttgttaattg atgcgctcat agtacctccg cttacacaag tattgacttc ttacaaacat
    40921 agtataaacg attggtttaa agaatgtaaa atatttaaca gtctgtgcat catctatatt
    40981 tctttcagtg acttatataa aaactggtat acttctccga atctcatagt tgcagaacat
    41041 ttcgggaatt ttggatgtcc ggtgaaagtt cttcatgcag ttacgtcata atttgaagga
    41101 ttaacaatct tgaagtattt tctgacagca gtctgttgct ggactgccct gtttctggca
    41161 gcatgcaatg aaaagacatt taaaaatgaa atacattctc ttcataaggt gccccctgaa
    41221 gagcgtattt ttccgcctgc ccttagcaca cgggagcggc tggggcttgg tgactccgca
    41281 caacatcatc ccatgatgat gcaatcagaa gagccggaag gcgaaagtga gcaccaactc
    41341 aaatgggagg cgccggtttc ctgggaaaag cgccctgcac aggccatgcg actggtcact
    41401 tatgctccga agggcagcgt atcatcagag tgctccattt ttatcctgag cggacctgca
    41461 ggcggtcttg aggcaaatat caacaggtgg cgctctcaag ttgcccagcc gcctttgtcc
    41521 gatgaagaaa ttgccgctct cccgcgtctt gcccttttaa acaatgaagc ggtgcttgtt
    41581 gaaatgtccg gtgttttgac caatgggaag ggagagaagg taccttcaat gatctacggt
    41641 ctggtctgtc cttatgagga gtccacattg tttataaaaa tgaccggccc tgtcgatgaa
    41701 atgcaaaacg aaaaagagaa tttccattct tttgcagatt cactgtactc cgatgaggat
    41761 gaaacagaat gaaaacactg aaaaagattc tggacttctg tgcatcctat ggtgttgctg
    41821 cagtgctgct gctgttgctg ttgctgcttg ttttcttcgg aacaatagaa caggcacgga
    41881 taggcctctt tgcggcacag caaaaatatt ttaattcttt tttcctgatt cattatattg
    41941 gcggggttgt tcctttaccg ctccccggtg gctatctgct tcttgctttg ctttttttca
    42001 atacgcttct gggggggata atccgcgccc ctaaaagatg gagcagaccg ggacttctca
    42061 ttgttcatct tggtatcctg taccttgttc tcgccggttt tgtagcacat aagtatgcgc
    42121 tttcaggtaa cctcccttta tttgaaggaa ggagcgcatc ttattttcaa agttattttg
    42181 actgggactt attcattacg gtaggcggag ccgatgggag cggtcaacag tatgttgttc
    42241 cgcaggaatc gttggcgcac ccgggtgcct ttttttatgc agaagaactt ccctttattg
    42301 tgaagataga taggtactat aaaaattcca tggcagttga agataactct gctcatgcaa
    42361 tagacggggc ggcactgaaa gaactgtctg agaatcaatc ggccgaagcg aatattcccg
    42421 gtatgctgat cacgctggaa ggtgacataa gtgaaaaagg gattttgtgg gggatgcagc
    42481 gggggccctg gattgttgaa gccgacggaa aacaattttc ttttgacttc acacgtcgcc
    42541 gctgggaatt gcccttcacg gtcactcttc agaaatttaa tcatgaatgg catcccggaa
    42601 ccagcacacc cagccacttc agcagtcgtg tttcggtcaa agatgaggga ctgccgcggg
    42661 aatcactgat tcagatgaat gaacccctcc gtcacagagg ctatacattt taccaggcat
    42721 cctggggtcc gtcggacgga aaagagggtg acccgctgta ttctgttctg gctgtagtta
    42781 aaaaccctgc cgggcaatgg cctgtctatg catcatgcat catttctgcg gggttactta
    42841 ttcattatct gcaggtttta ctgcgctatc tgaaacggcg ttcccgtctt tcaggtgaga
    42901 aggggacatg ctgatgccgc attttactct attttcccgt tctttaattt tcctggttat
    42961 ggtgagtgct tttgcgggtt ctgtcttttt ctctcaggaa gcaggtgcct cctcctggaa
    43021 taaagatctt gtgtatcttt tcaagacctt acctgttcag gaaagcggtc gtataaagcc
    43081 tctggataca gcagcgcggt tcatgttgct gcggatgaat ggaaaaagaa ccgtcagtgt
    43141 gaatcagggc ggcaaaaata tttcgctttc agcaacagaa tggcttcttg actgcctatt
    43201 tcaccctgag gcagcccggg aatacgcaca tttctctgtg gattcttatg agctgattac
    43261 ggcgatcggg cttacgccac acggaaaaaa aagagatcgc tattcctact ctgaattgga
    43321 gcctgcgctg gatactttgt ttgctcttgc tcatgaagtt tccgcccgcc cggaaaacat
    43381 acgcagtctt cttgacgaac agctgattgc ccttgcaaac aatgttttgc tttttgatga
    43441 actgacccat ttcatggatt ttgcacgcct tcgctttgac gttgcgaaaa gcgcacttct
    43501 gacgcttctt ttcccggagg atcacgaggt tcctttaagc aaagtagtgg agaaattgcc
    43561 cgaccaatta aaaagcctgg tcgatcaggc cgccgatatg gacgaggaaa gaaaacggga
    43621 aatcgccaaa gatcttgagg cagtgttcca gcgtttggat gaggtcagtg catcggggca
    43681 gatgcttgcc ctgtttcctc ctctttcccg ggaggaaaaa gagtggatga ctcctgcaaa
    43741 gtatctggaa tccgccttta ttggcatgcc ggcagatacg gtcagcggtg atattgcgct
    43801 ttttgaaaca ctggcagatg cggcgcctga ttcgggcgcc tttgtgagtg ccgtgaaacg
    43861 tttgcacggc cgcattaccc aggaggcaca agcgcgaggt gagtacaaac atattcccct
    43921 tgaagtgcat ttttatgatg cccgctatct ttttttcagc caatggcttt ttgtactgtc
    43981 ttttttcctg atggcttttt cctggacccg ttcgccggac agcagactcg cacgcgttct
    44041 gtccttctcc atattgcccc ctctcattct gctgggtatc agtattaccc tccgatgcat
    44101 tatacggggc agaccgccag tgaccaccct ttatgaaacc gttctcttca caacctttgt
    44161 tgctgttgct atcggttttt tccttgaatt tatttctaaa aacaagatcg ctgtttcggt
    44221 gtcggcgttg ctgggcacaa tcggaatgtt tttcgcctgg agatatgagg caagcgaagg
    44281 cgtggacacc atgcctgcag tcattgcggt tcttgacacc aatttctggc tggctacgca
    44341 tgtaacaact attattgccg gttatggtgc aggacttttc gctgctgcgc tggcgcatgt
    44401 ttattttgtc ctgcatcttc cgctttggaa agaaaaagac tctgccgcca tgcagacatt
    44461 ggctacctcg atttacgggg ttatggcttt ttctcttgtg ttcacactga taggcaccat
    44521 cttaggcgga gtctgggcag cgcagagctg gggccgcttc tggggctggg atccaaaaga
    44581 aaatggagcg cttatgattg tcctttggat tctggcggtg ctgcatgcgc gtcgtggttc
    44641 ttacattaag gcgcgcggca ctgctgtcag cgcactgatc ctgggcatga tcgttgtctt
    44701 tgcctggtgg ggcgtcaatg ctttgggcgt ggggcttcac agctacggat ttaccaccgg
    44761 catctggaaa agcctgggcg ttttctggat cgtggaaaca ttttttattg ctgcggcaat
    44821 aggcatcagc gtgctctttc cacgaaaaga gatgcccgtg cagaagggaa gtgctcagcc
    44881 ttgagaaagg gcatgtattt ccgccataag aagcggcagc gtctttccgg cggggccctg
    44941 aataaaatga tccacaacga catcggaaaa ggctgttttt tcaatattgc attcgatgac
    45001 aaccgctccg ccttcttttg ccatataggg caaactggcg gcagggtaca cctgcgcaga
    45061 tgtgccgact acaatcatga cgctgcagcc tttggctagc atttccgacg tcttcagtgc
    45121 atattcggga atgatctcgc cgaagagaac gatgtccggc ttcatataac cgccgcactc
    45181 acagcggggc gctccgagaa cgcgctgact taagtccata ggcctgcgtc gggtgcaggc
    45241 gggacacatg atctgagctg cgttaccgtg atattcaatt acagtgcggt tgccggcttc
    45301 gtggtgcagg ccgtcgatat tctgtgtgat aaccgcttta agatgcccca tatcctcgag
    45361 ttctgcaagg gtcaggtgag ccgggttggg ctttgctttt ttgagcgtct cgccgaggtc
    45421 ataccacatt tcccagactt tatcagggtt gtcgtaaaag gcgtccagcg tcgcatattc
    45481 ctgaggcggg taccgtgtcc agagaccgcc cgggctgcga aaatcttcta taccgctttc
    45541 tacggagatg cctgcgccag ttactgcaac tgcattggta gtctgaaaga tggcacgtgc
    45601 cgcttccttt acaacagcct caagattcga catgtaagtg caggactcct aatgctgagc
    45661 cgggcaatgc gccggccggt gttttatgtc tgagcaggaa taaccgaaag accgcccata
    45721 taagggcgca atgcctccgg tataatgaca cttccgtctt cctgctgata gttttccaga
    45781 atagcaacta ctgttctgcc cacagcgaga ccggagccat tgagcgtatg aacaaattcc
    45841 ggctttttgt cccgtttgaa gcggatgttt gcacggcgcg cctgaaaatc cgtgaaattg
    45901 gagcaagaac tgatttctct gtaggtgttt tgtgcgggaa cccagacttc caaatcgtat
    45961 gttttggctg ctgaaaaccc gagatctccg ccgcaaaggc acacaaccct gtaggcaagc
    46021 cctaactgtt gcagaatata ttcggcattg gccgtaagtt tttccaactc ttcccaggaa
    46081 ctctccggcg tggtgaattt tacaagttca accttgttaa actggtgtac tcggatcatg
    46141 ccccgtgtgt cttttccgta ggagccggct tcgcttctga agcagggggt atgggcgaca
    46201 tagtatttcg gcagatcttc aaggctcaga atctcgtcac ggtggatatt ggtaaccggc
    46261 acttccgccg tagggatcag ccaataatcc gtattatcaa ttttaaaaag gtcagctgaa
    46321 aatttcggca gttgtccagt gccttgtaca ctcgccgtat ttactagaaa gggcggaagc
    46381 gcttcctgat atccatgttt ctgcgtatga atatccagca taaagttaat taaggctctc
    46441 tcaagccggg caccgtagcc caggctcagt gtaaaacggg cacctgaaat ttttgcgcca
    46501 cggctgaagt caacaatgcc gagtttctca gcaatatcca catgatcacg gacttcaaaa
    46561 ttaaatgagg gaatttcacc ccagcggcgg atttcctggt tatccgcttc gtttttcccg
    46621 gctggaacgg attcatccgg aaggttcggc agggatagga gaatctcctc caggtcttct
    46681 tctgcctgtt tggcaatcag atcgaggttc gccgattctt ctttgatctg cttcatctcg
    46741 gcgatgacat catctgcgtt ttcgttgttc tttttacgtt gcgctatagt ttcgcttgct
    46801 ttattctggc gtgcacgcag ctgctccatt tcataaacag ccgagcgtct tcgggcatcc
    46861 acagccaaaa tggtgtcaag atcaacaggc gcattgcgct tttccatggc ggcgcgcacc
    46921 agttcgggat tgctcctgat gagtctgata tcgaacatgc actattcctt tgctatctga
    46981 tcatgtgttc cagcaggcga tccagttcat ccagagaaaa ataagcgatt tcaatggtgc
    47041 ccctgctgtt atcgacaggt tttaaattga cttttgtgcc cagcccccgt ctgagtcgct
    47101 cttcgagctc ctgcatgtag ggttcttttt catttttttt ccgcacgctt tgcgtctttt
    47161 tttcgccgaa tttggcaacc atctgctccg tctggcgaac tgacagtccc tgagaaataa
    47221 tcttttgcgc tgccgcctgc tgcgcggagg cggtggaaag ggagagcagc gccctggcgt
    47281 gccccatgga taaggaaccg tcggcgacca gttgctgcaa tccatcggac aattgcagga
    47341 ggcgaagcgt attggttacg gtcgcacgtt ttttgccgac ttcaaccgac aactgctcct
    47401 gtgtccaccc atattcttcc atcaggtctt tataggcctg tgccagctca atagcattca
    47461 ggtcttcccg ctgaatgttt tcgataatgc cgaacttaag catttcctgg tcggaaatat
    47521 cacggcagat cacgggcact ttctgcagtc ctgccatgac agctgcccgg cagcggcgtt
    47581 caccgcagac aatctcatag gacccgttat taaaacggac gatcaaaggc tcctgaacgc
    47641 cgtcgttttt gatggattct gcaagctcac caagagattc ttcgttaaaa tgctttcgcg
    47701 gctgtctggg attaggctta atctcttccg gattgaggtg catcagtacg ccgctggcag
    47761 gcccggaaaa gttttctctg cggacatcgg atattccctg ttcaggcggg ataggatttt
    47821 ccatgacatt acttggaaga agagcattca gcccgcgtcc cagccctttg gtcgtttttt
    47881 tctttggagc atcagtcacg gatcaaaacc tcttttgcaa gggcgccata ggcctgagcg
    47941 cctgttgatt tcggcgagta atgattgata ggcatcccga agctgggcgc ttcgctcagc
    48001 gccacattgc gcggtataac ggttttaaat accttgccca taaaatggtt gcgcacatcg
    48061 gcttcaacat cccgggacag cacggtgtgc tggtacatgg tgagaagcac gccgaaaaca
    48121 ttcaaatgac tgttgagagt gttcttgaca ttaatgatag tttgcagcag ctcggaaagc
    48181 ccttccagcg caaaatattc acattgcaga ggcgccagaa tgccgtgggc ggcagtcatg
    48241 gcgttcaacg ttagtacgcc caatgaaggc gggctgtcaa taaaaatcca gtcaaaacgg
    48301 ctgacagcag ggcgaagggt attggcaagg taaaagccac gattttccag ctgtgccagt
    48361 tcgatttcag ctcctaccag atcagtcgta gaagcaacca gagataaatt cttataagga
    48421 gtctgcacaa tggcattatc gatgggaaca tgtcccgtta atgcctgata aattgtggcg
    48481 ggaaccgtgc ttttattaat tccggaggcc tgactggcat ttccctgagg atccagatcg
    48541 atgagaagaa cgttggatcc cagctctgcc agacaggcgg caaggttgac ggcagtggtt
    48601 gtttttccga caccgccttt ttgatttgcc acagctataa tgcgtgtcat acaataattt
    48661 ccggttttgt ggaattaaag cctgaagaac gataataata cggttaggag cgtatctaag
    48721 tcaaagtccc tctgaaccgg tcaaaatgtc caaataagaa aaaacaaagc ctatattttc
    48781 tcatcttcac cctgttccgg gaatccattg ttcatacaat tgtgtttcag cctgttgaag
    48841 cagcctaatt tccggacacc gagtgtattt tcgccaatct gttccaatag tcaggggttt
    48901 tcatgaccat tgccatcaca ggccttagcc accatacaag cccgttagcc ttgcgtgagc
    48961 gcatggcatt gcccgtggag cgcatcccgg cgatgctgca ggatttcaat gcgcggtttg
    49021 gagccgacag tggctgtttt attcttgcca cctgtaatcg tttggaagtc tatgtgcacg
    49081 caagacaaaa tacggaggcg atacaccggg agataaccgc ttttctcgga aaagcgacac
    49141 ggcttgaaga atcagaattt tccgatttcc tttaccatta cagcaatgaa gcagccgtaa
    49201 aacatttatt ttccgttgcc gccagtctgg atagcatggt agtgggtgaa aacgaaatta
    49261 tggggcagat tcagcgggca catgatcttg cttccgaggc gaagacactg aaccgccaac
    49321 tttccatgct tatccaacgt tccttgaaat gcgggaaaag agtgcgcacg gaaacagcga
    49381 tgggcaaagg aaaagtgtct gtagcttctg tggcagtgga tctggcggaa gcaaaactca
    49441 agcagctaac gggtaaaacc gctttgattg ttggctcggg gcaggtaagt gagctggctc
    49501 ttaaaagtct tgtagagcgc ggtgtcgaga gagtacttgt tttaaatcgc accttagcgc
    49561 atgccgaaga acttgcgagc aggtatcagg gagaagcgct tattctgcag gctttgccct
    49621 gccacctgca tagagctgat attgtgatca gctcgacggg tgcgtctgat attattttaa
    49681 gagccgtcga ttttcagcgt gctatcgaaa aacgaggacg tgcgcccatg ttcgtcatcg
    49741 acattgctgt cccgcgggat attgaaaaag aagcaggtcg tatcgataac gtctattgct
    49801 acgacatgga cgatttggag ggtgtggcgg aacggaattt acgaaaaagg caggacgaaa
    49861 ttcctgcatg ccttgaaatc gttgacaagg aaagcctctc ctttcttcag tggcagcgtc
    49921 gcctttcatc cgatccctta atcaaatcgc ttacgcagtc tttcaatgca atccgtgaga
    49981 aggagctggc acgcacttta tccgaacttc ccggactgtc agaaaaagaa cgggaaaaac
    50041 tggagcagtt ttcaaaacga attacaaaca caatgctgcg tatgcctttg cgtgccatta
    50101 aagaattttc acaaatgccg gagcacgaat tactggtgga gcatatccaa acggtgtttg
    50161 gctggggaga cgccaaagta gaatgaaaat tattttcggt acgcggggca gtgcgcttgc
    50221 gctggcgcag acagaacaga ttatggcgtt gttttccgct ttgcatccgg ctgtggaagc
    50281 ggaaatgaag attattaaaa cgtcagggga tcgggatcag cattcttcgc tatcgtcgct
    50341 gggaggctgg ggcgcattta ccagagaact tgaaaaagaa ctgattgccg gtacaattga
    50401 tgcggctgtg cacagcctta aagaccttcc tactgaacag ccggagcccc tgactattgt
    50461 ggcggttgca gcgcgggcac cagtagaaga tgtcctgctg accaatgaag actttacgct
    50521 tgatacgctg ccttcagggg ctgtggtggg tacgtccagc ctgcgacgcg aagtgcagct
    50581 gcgccgtttt cgccccgatc tctgcataaa agatattcgc ggaaatgtcc aaacacgcat
    50641 agataaactg cggcgacatg aatatgacgc aattcttctt gcccgtgccg gattatccag
    50701 gctggcgttg ctccaggaaa aaggtttata tatcattcct gtaaccatga tgattcctgc
    50761 tcccgggcaa ggtgccattg caattgagat ccgcaaatcc gacaaccttc ttcaggatct
    50821 tttgctgcca ctgaacgatg aacagactgc tgcctgtgtc caatttgaac gcagcgccct
    50881 tcgctccttc ggaggcggat gcaggtcgcc gctggcaata cacgcagctc cgcatgcatc
    50941 cgggctggaa tttcagtctt ttgtggcgga gagccgaaat cgttatttct tctttcataa
    51001 agcgctgtct tcctggaata ctgtcacaga agagggggat gcgttggggc agatgcttca
    51061 atcggtgtca gtccgaaccg aatcggcgca caaaactccg gaagatgctc tttcggataa
    51121 aaagattctc gtaaccaggg cacaccacca gagcaaacct ttttgtgata ttttacgtac
    51181 ccacggcgca gaagtgttgg agctgccctt catcagtatt cagggagtcg cttttgaaaa
    51241 accgtcactt aaggacagct ggctgatctt caccagtgca aatgctgtgc atcacttcgt
    51301 aaatccggag ggaacagatc ccgcttcctg ccgctttgaa agggatctgc tgattggagc
    51361 gattggtacc ggcacagcag aggctttgtc tgaatatggc gtgacgacac cgtatattcc
    51421 gcaaaaagga acagcgaaag tatttgccaa tgaactgaca gcgaagaaaa aaatgtccgg
    51481 gataacaaaa gcggtcattc catgcggaga taaagccctc cctgaattat cggaattgct
    51541 cgtgcaccag gggtggacgg tagaaacacc cgtttgttat cgcaccgtta ttgcccggct
    51601 gacagagggg gaaaaacaag cgataatcaa cttccggccc gatgtaatca ccactttcag
    51661 cccgacagcg gtggaaagta tgatgcagaa tggaattccg gaaaacctgc agaaagtaca
    51721 atgtgctcct ctatatgcgt ctattggacc cacaacgacg caggagcttc agcggagaaa
    51781 ccgcactcct gtacttgaaa gctttcttca gtccggtaca ggcttgtgtg atgctttgtg
    51841 cgcttattat gaagggctgc actaatcagg ctgctcaagt cttgtaacgc ttgaccgcac
    51901 gttctatttc ccggtctgtc tctctctgct taatggattc ccgtttatca taaaggcgtt
    51961 ttccacggca caaaccgatt tctactttta cttttccctg tttaaaatag gcggaaagag
    52021 gaataaccgt aagtcctttt tcttctaccc gttttatcag gcggttgatt tccatttttt
    52081 ttagcagcag cttccgtctt cttaccggtt cgtgattgtt tctgttgcct ttgtcatagg
    52141 ggcttattct ggagccgaca agccatgcct cgttttctgt aaaatccaca tatgcctcct
    52201 tcagtgaaaa tgtgttatgc atacgaatgg atttgacttc ggtgccttca agaacgatgc
    52261 ctgcctcgaa gctgtcgaga acagtatatt catgaaaagc atgcctgttt tttgtaatga
    52321 attttttgcc ctctttcatc gttgcccccg ctgcgcaatt tgtatggcat tgttatcggc
    52381 ctgatcggat agacctatcg tgacttttgc ttccagatgg aaagccttgt ctgaaacaat
    52441 tccaagcaca gtaaattcct gcagtgtgga aatttgtttg ctaattttcg ttgctgcttc
    52501 tgcaaaggaa ctttccgtat gcccgcgcaa aagtccggat gcggcaaact cattaagggc
    52561 aggcacggct gcctcccacg cttgagccgg gcgcagatgg aaatagaggt ttgctttttg
    52621 ccccagctga gcggtgaggg cctcgctctg cattagtttg cggataaggg tttcgcttgt
    52681 ggcaaagagt gcgccttgag gataaatggc gagagaagga gtcaggtttt ctccgcctat
    52741 gaagggtaca gttgccagga gcagctcact gttaacagaa ggattcagga caatcatatc
    52801 gtcagaaaag ggtggaggag gtacgagatc aaaagcggcg tgaagtgcat ctgtttttcc
    52861 ctgtgctgtt ccggccaaaa gtggcgcggg aactacttcc agaggatcaa aaccaaacca
    52921 ggcaagccga atcttttctc cggaagaatg gaaaaaactg gcgataagat actggagcag
    52981 ttcagtggca gaagcatttt caggcagtga ctcggagagg gttatccatg aagagacctt
    53041 ctcccagcct tcattggttt ctgccagctt tgcataccat tggggcaggg gtgccccgag
    53101 attcactgaa agagaggcta ttccgtcaag cggcatatcc agagaatcgt ctttagtatt
    53161 ctgcggtaaa agtgttccgt atcttgccag tatttcatcg gaaaaagaac ctgtaatcga
    53221 aaggcgcggc acggactgat tcaggcgcac agaaaaagac agatccttga aaagagcggg
    53281 ggattgcaaa aaagccttcc cgtccagacg gcaaaacagg tctgcctctt ttgccttctg
    53341 ctgtgccgct tcaaatttgc ttttttcaat ggcttcctcc ggtggaagcg tcaaagcctg
    53401 gatcagggca tctcttgaag agctgaccag aagatatttg tttctgacag agtaaaatat
    53461 ttcagcattt attacttttt tcagatcaag tccgccggcg cgttctcgcg atacgttacc
    53521 ggacagcgaa aagaatttct ctgatagcag gcctatacgg gtcattcgcg taattacgag
    53581 caaactgtcc ggacgattgc tgtcttcgac agagatatgg aaaagaccgc tgctgacatt
    53641 attcagtaac caatcgggga caggtaattt acgcgttaac aggtgaagat aagcagctgc
    53701 tgctgtatct tccggaatcg tgtgcagaat cgggcttttc aacaactcat tgcgcttggt
    53761 ggcagggtcg taaacgtata cttcgatgct gtgactggcg ggaatgaaag attccatttc
    53821 cgctgcatgg cgtgccggta tgaaaaacca cacactcgca agcacaataa gaacaaaaat
    53881 aatgaacagg aaaggcttgc ctgaggacga acgttgaatt ttaggcttgc ttgcggtacc
    53941 gccccagaaa gtcaattgaa ctgaattact catggctgga tatctccgat acggtcaacg
    54001 ttgcaaaact gcctgccttg ccgtccggtt gtttgaaagc acacattatt tcgggctggg
    54061 atagttgtaa caccacgcaa tgccgggagg cgtgattcca tatatgctgc ataccgccgt
    54121 cactttccat aggggaagcg gtcagcagag tacccaggga ttctctgttt ttgaccggtt
    54181 ggtttttcaa agtttctatc agtgcctgat agcgctcccg tgtttcatcc gggattccct
    54241 gtgtgtcagg attgactccg gtgagtatgc ctttcaccgc ctttcggaca gaaatgcttt
    54301 gtccgtattc aacgaccgcc gcatctgctt tctcgccatc atacccggca ataaggacat
    54361 gatagttctt aatatactca gcggcggaaa ggactttcag cgcttcgctg aaggaagaac
    54421 agctgcgcag caattgaccc aataaaaact ccaacggcgc tttctccgga agaacagtat
    54481 cgacgtaggg gacatattcc agacacagga aaagcctgtc ccggtttagc ccggtcatca
    54541 gaccggcgtt ccagtcaaag cctacttgaa tagaaggcag cccttcttca ggcagcatgc
    54601 ggatgacata atcattttcg tcgcctttcc agtccagatt tcggccgaga agaatatctt
    54661 tcgttcctgt gtattcgctt gttaaagcca ccatggtgca gagcgggaga gagaaaaggg
    54721 aagactgatc tttggcaagg ctgtagagcc tggcgtgctg gagcagccaa agtccgtcaa
    54781 agggaagtcc tgccccttca gccatgcctt ccatttcacg gatatagctt tcagacaatc
    54841 cctgcagcag agaacgtgat cccatcccta gtgctgtcat tgccaggggc gtcaaattca
    54901 gaaaagacgg catcgccgtc caccaactgc ccgtaggata ccactgcccg gtatccacgg
    54961 gatccagcac ttttgtttta tatttgttct ggatggcatt atgaaaaaaa ctgccccgcg
    55021 ccaacccaat gtcgtggctg gacccatgca aagtaagcaa aggaaaactt gcatcacctt
    55081 cggattgctg ggactcctgc gcctcttcgt ccggcaaatc aacaatggat tcgaccagtg
    55141 tttttcgcga catcaggctt tcaaactggg aataaagcca gtcttccatg cccggtccga
    55201 aaaaattcat aaaagattcg taattggcaa atggataaag agcacgaggc acaaagtaca
    55261 tcaggtaatc attggcaagt cccacagacc aatgggcgtc atatccacgg gcaagcgccc
    55321 gttctcttag attcaacccg atttcaacgc agggttcccc cgggaaaaaa gagatcagta
    55381 aatcattgat ttcaagtgaa tataagtcca cttctttggg ctgcaagttc tcagcaagcg
    55441 taagtggcag gagtgccttc gtatggctta atttaaagac ggcatctccg aaagacatgt
    55501 ttttggacag cgcatcggca atttgcgcta cctgcctgcc gacggcttcc gtgcgatccc
    55561 agccgctttt accttttggt gctgcgatgg tctgatttcc ctgcgcaccg tttaaaaaca
    55621 gaggaacgca ggattcttcc gcaagcgcct ccatttccag gtaatagtaa ccggggtagt
    55681 ctgcagaaaa agagtactta tcctgcccac cgacagaagt cggatgaccg gtgaaattgg
    55741 taatgacagc aatggcggca ccgtccgagt cttcaatttt taccacgcct atctggcttt
    55801 cggcagggcc agcggaaaaa cgtctgttaa cagacaggcc ctgattactg ccgacggcat
    55861 atccgagtgc tgcacgcctc cggccgttat aggctttgtg catggattca ataatttttt
    55921 cagcagtcgc ctccagaacg gcgggaataa agcgtccgga aacattgcgg atcatcagtt
    55981 tttggcacat gccgccgtgg ccgttatgcg tatgggttgc tgtgagcagc acatgttctt
    56041 taggtacagg cccggaatac agttcaagaa cacgctgacg caattcggga ttaacagcga
    56101 caagatccag gctgaccaga aaaaagcgtg tttcgccgtc atccagaaaa acggtgcgtg
    56161 cccacaacgg atcatgaacg ctcgtagaat tgcgtcccat gcggtcgccg taaccgttta
    56221 agggcgtgcc tacaggcggt gtgatgtcga ttttggcaga ccctgcttca agtgcccaac
    56281 tttgtacggc cgataggaga ataaaaagga aagcggcgaa atacagagct gttcctcggg
    56341 aaagaaagtt taccttaagt cgaaacagcc tgaaagggat agaaagggga catcggtttt
    56401 tcattgttta ttcctgcgtc ttcagggcga cggttaaagc cggtggtaag agatcacttt
    56461 ctttagtgcg gatacaactt cctgcacatg acggtcattc aaaccgggat gcagcggaag
    56521 ggaaataatg cggtcggaaa catctgttgc ctgaggaaaa tcttcaggcc gcatcccgag
    56581 cacttctctg tagtattgat gaagatgaag tccgtaaaaa tggatgccgg tgcctatgtt
    56641 ttcccggcgc aaagcttcta ttacactgtt tcgtgaaaca gaaagagtat tgggcctgat
    56701 tttgatgata aacagatgcc acgcatggcg cccgtcctca gcatcgggag gcatttccaa
    56761 ttcaggaaca tctgagagta cagacaaata taattctgca atacgcttgc gtgcagctct
    56821 gaattgttcg aactttttca gctgctctac gccgagagcg gcagagacgt tgcttaatgc
    56881 atatttgtag ccgggttcca gcacttcagc aggacctgag acagcgcttc ggccgtaacg
    56941 gtcccaggca gagctgccca tcccgttgcc cgccagacgg cgaaggcggt gtgccagcga
    57001 ttcatcatcc gtggtgatca tgcctccttc catggtggtg atatttttgg tggcataaaa
    57061 gctgaagcaa gcgagacctt cagcgcccac aggcttcttc ctgtattcac ttccgaaagc
    57121 gtgtgccgca tcttctatga caggaatacc gtgccttttc gcaatcgcac gaatcggatc
    57181 catgttgcag gaaagccctg ccatatgaac aggaataatg gcgcgcgttt tttccgtgat
    57241 tgttgcttcc agctgcaaag gatccatatt cagagtatgc ggatccacat ccacaaaacg
    57301 gacggttgca cccattgtta aaatagtgtt gcctgtagac gcccaggtga tcggactcgt
    57361 aattacttcg tcgcccggtt ttatagcgag cgccgccagg cataggtgca gtgccgcagt
    57421 gcaggatgaa acggaaacgg cgtaaggtgc gttcactttt tcagcaaaac ttttttcaaa
    57481 aaaagtgatc tggggacctg atgtcagcca gccgctgcga agaacctgca gcaccgcacg
    57541 ctcttcatct tcacccaggt taggcttgct aagagcaatg aacctagctg ctaccggctc
    57601 tccgcctgca tcaactggcg ttgccctgag ttttttcatc cgttcaacat tgaaaaatgc
    57661 ttcagagaat ggattgatgt ccggattatc cagatatacc tttaactcgt caatcccttc
    57721 ctcaaaggaa atctgctcgg ggagaggaac agtttcggca atttttgaaa aacgaacttt
    57781 atagttgcgc tggtcatcat catcccgcgc agtcgtcaca gggacttgag aacatcgggc
    57841 tgccactttt tcagcaagag cagcgatagt acaattctgg gtatcccagc ccacattgaa
    57901 aataccggac ttgcccgaca gatctgtgtc ggaagcaaga gacacaagga tttgtgccgc
    57961 atttctaaca tgaatgaaag ggcgccattg gtttccaccg cctttgacca gaatagacct
    58021 gttgcggaca gctgaggcag tcatgtggtt tacagcaaga tcaaaacgca tacggccgga
    58081 aatgccgaaa agcgtgccca tgcgcgcaac ccgtggcgta aactgagctg aagacatgtc
    58141 caaaagcgct ttttctgctt ctattttggt ttccgcaaaa acagatacgg gatttacagg
    58201 gctttcttca tcaagaatag ggaagacgcc ctgcccgtac acagcgcatg tcgatgcaaa
    58261 gacaaaaggt cgtataccgc tttggatgca ttgacgcgcc aactctctgg tacactcgac
    58321 attgacttca taggcaattt caggatccag agcacatgtc ggatcgctgg aaagaccggc
    58381 aagatggatt accccctcaa tgccgtataa caggttctgt tgctcctgca accggcggat
    58441 atcgccgggt atgatttcca gattccgccc gaagttgttt tccgcgaaaa gcgtttccgg
    58501 agtcattcct tcggaaagga aggaaaaacg gtcaaatacg cgtaccacat gaccctttga
    58561 aagagcctcg cgaactaccc agtagcccag gtatccgccg ccgcctgtga ttaaaagtcg
    58621 catggtaagg aacactccgg agaaatatag tttttgaaaa gaggcccgac actaaaaact
    58681 acctacgaga aaccggagaa aaagaaactc cggaaacacc tgcttcgttc aatcctgttt
    58741 ttgcataatc agccttattt tactaaaaaa aaccagcatg acgctaagta tagcacttag
    58801 tgaagaatgt cctgtaacag cggacatatg cacacttgcg gaaattgttt tttagtagac
    58861 aaagtaatat aatacttaac aggtcgtgta taataaggag caggaatgag agtatcaaaa
    58921 aaatcagact atgccttaag ggtgctcgtc tatctggctg aaaaaatcgg ggcacgtccg
    58981 gtttcaatcc gtaccctggc aaggctccat gacattccct accgtttttt gcagcagatt
    59041 gttctcgata tgaaggagca gggatggatc atgacgcttc cgggccggga gggtggtatc
    59101 caacttgcga aagagccccg ggaaataacg atgggtgagg tagtgcggtg ctttgatggt
    59161 gttttggctc ccattgcctg tgtgtcaatc acgcaaaatg aagcatgcag tcaggaagca
    59221 tactgtaaat tccggcgaac ttttctcgaa ataagaaacc acaccgctca gcgtatggac
    59281 aagactacct tacacgatct gactttctgt gttcctctgt cgcaggaaga agtgtttggt
    59341 gttcaattta aagacggtgc agggatttga aagagaatac aatgccattc gtgcgcttaa
    59401 tttctttgtg tctcttcctg ctctttttaa gcggttgttt cagttacagc gggaaagaca
    59461 cggctgttga gattttgaat gtgtcttatg atccgacccg tgagttttac cgggagtata
    59521 atgcctggtt cgaaaagaag gcgtttgaaa gaaccggcaa acacgtgcgt attctgcaat
    59581 cccacggcgc ttcaggtaag caggcgcggt ttgtcattga cgggctggaa gcgcacgttg
    59641 ttacccttgc gctggcttac gatatcgatg caatagcaca aaaaagcggc agtttaagca
    59701 aggattggca gacacgtctt ccctttgcga gtgcaccata cacctccacc attgtttttc
    59761 ttgtccgtaa agggaatcca cggaatattc aggattggaa tgatttaatc aaacccgggg
    59821 tacaggtcat cacgcccagt cccatgacct ccggtggagc gcggtggaat tatctggccg
    59881 cctggggata tgcctggctg atttcgctgg agaaacgcgg agtcattctc tctgctatca
    59941 cagaagacga acgaagacag gcagatgagg atgctttttc ctttgtttcg gcgctatttc
    60001 gcaatgttcc tgtgcttgat tcaggtgcgc gggcagcgac gaccaccttt gcccgccggg
    60061 gaattggaga tgcgcttgtt acctgggaaa acgaagcgtt gcttgccatg aaatctcttc
    60121 ccggcaaagc acttgaaatt attacaccat ccataagcat tttgacagaa ccagttgtcg
    60181 ccaaagtaga tgaagtaacg gcacgacgac aaacagatgc attggcacaa agttatcttg
    60241 aaatgctgta cagcccggaa ggtcaggcaa tggcagcccg gcatcatttc aggcctcggc
    60301 atatggaatt tgtcaatgca gaagataaaa accaatttcc tgatttgcac ctttttactt
    60361 tggaagaact ttttaccgac tggcaaacag cccataaaaa ccatttcagc gaaacaggca
    60421 gcttcaacaa aatctatcaa agcctttatt ctctggagaa ccaatgattc ggcagataaa
    60481 acaaaagcat tttttaccag gattcggcat tggattaggg tttaccttgt cctacctttc
    60541 attacttgtg ctcattccgc tcagtattct tgcggtaaag tcactgagcc ttgactggac
    60601 agtctaccgc tctcttttaa tcgacccgag aacatatgct gcaataaaac tgacctttct
    60661 ctcttcgctt gttgctgccg ggattaatct ggtttttggg tttattgttg cctggacact
    60721 tgtgcgcctt ccctttcccg gaagtcggct gatcgatgct cttattgatt tgccttttgc
    60781 gcttcccacg gctgtgtcag gcattgctct gacatctctt tatgtaagaa cagggctgct
    60841 cggcagatat ctggaaccct acggtgtaca ggcagctttt gctcctttag gcgtgatcat
    60901 tgcccttact tttatcggtc ttccctttgt cgtgcgaacg ctgcagcctg cattgcagga
    60961 tatagacaaa gaatatgaag aggcggcaat cagtttgggt gcttctccgt ttcgtgtttt
    61021 tgcgtccgtt attttccctt ccatccggcc ggcaatgctc acaggatttt ccctggcttt
    61081 tgcccgggcg cttggtgagt atgggtccgt agtttttatc tcaggcaata tgcctatgaa
    61141 aacggagatt gtttccctgc tgatcgtagg gaaaatggag cgttttgatt atgaaggggc
    61201 aacggcgctg gccgcaataa cccttcttgc ctcttttctc attcttcttg ccatcaacct
    61261 tacacaatgg cattacagcc gcaaagttca gcagacaggg taggcaaatg gataccgata
    61321 tgcgcagctc catgcagagg cttattcaat tttttatgcg gtgcattaca atcacttttg
    61381 ttgtgctttt tctggtgctc cccttgtatg tgattttgag tgaagcgacg ggcgaaggga
    61441 tgagcgtgtt tttccgggct gtcacacatc aggaaacgtt gcaggcgctc actttgacta
    61501 tatgtaccct ctttatcatc ctgcctctaa atatcctttt cggcatcgca gcggcttgga
    61561 cattgactaa atacgaattc agaggcaaaa gtgttttgtc cgctctgatt gatcttcctt
    61621 tttcagtgcc tcccgtaatt gcaggcttta tcctgattct ttttcttggg cgacatggga
    61681 ctttgggctt tctgcttgaa aaactcgata cgcagattat ttttgccgcc cctgccgtta
    61741 tcgttgcgac cttgtttgtg actttcccgt ttgtcgcgcg tgaacttgtt cctctgatgc
    61801 aggagcaggg tgtcgacgaa gagcaggcgg ctgtgtcgct gggtgccagt gccttccaga
    61861 cttttttcca tgtcacactt ccaaatatca agtgggcact cttttacgga attattctga
    61921 gcaccgcccg agctatggga gaattcggag ctgtttccgt tgtgtccggt catattcggg
    61981 gaaagacaat tacactgccc cttcatatag aaatactcta taacgaatac aattttacag
    62041 cagcttttgc ggtttcctct ttgctggtta ccctggctat gagcacgctt gttgtcaaag
    62101 aaatcgtgta ctggcgttcc ggcaaaaaac agacacgacc aaccggcggt tttttggaat
    62161 cgcctgagac accagactta tgagcattga agtacaacat atttcgaaga gtttcggttc
    62221 ctttcatgcc cttaaagatg tttctttacg agtgaaatcg ggagagctta tggctttgct
    62281 cgggccttca ggctctggaa aaaccagtct gttgcgtatc atttccggat tggacagacc
    62341 tgatgtgaac ccgctggcta aagtgatcat gaataatgaa gacatgatca gtaccagtgc
    62401 tggcaacagg cgagtgggct tcgtctttca acattatgcg ctttttcggc atatgactgt
    62461 ttttgacaat attgcttttg gcatgcggat gcgtcgaaaa aaagaaaggc cttccaaagc
    62521 ggagattaaa aataaggtag agcagcttct gcatttaatt caactggata atatgggaaa
    62581 ccgttacccg ctgcaactgt cgggaggaca gcgccagagg gtcgctcttg cgcgggcgct
    62641 ggcaatagaa ccaaaggtac tcttgctgga cgaacctttc ggcgccctgg atgcacgtgt
    62701 aagggaagat cttcgacggt ggcttcgccg ccttcataaa gaaattcaga ttactacact
    62761 ttttgtaacg cacgatcagg aagaagcgct ggaactggcg gacagagtcg ttgttatgaa
    62821 tgaagggcgc atcgaacaga tcgggacacc ggaagaggtt tttcattctc cccgaacaga
    62881 atttgttatg aaatttctgg gaagcgtcaa cttatttcac ggaaggatcg agcgaggcaa
    62941 agcagttttt ggctcctgga aagtgagcgg cccggaggct ggcaaagagg aaggaacgcc
    63001 tgcacggctt tttgttagac cctacgacat gaccattgtc cgggaaggag accccgccca
    63061 cggaagcaat gcggtgattc ggcggattca gtctgccggt ccttacgtga agattgaact
    63121 tgaacatgag agcggagaga acatcatcgc ggaaatgtct catgaacggc atgagagtct
    63181 taagcctttg gtgggcgaga aagtagcggt aagtttaaaa aacgttcggg tttttaccga
    63241 agactacacc atataagtac attaacggtc gtcagactgc tgcgcggcat accgtcttag
    63301 tttgcgccac agcgtatttc ttccgatacc cagcagtttt gctgcctgca gctggttccc
    63361 tttgcagtgc cgcaatgcca ggcgtatatg cgcctcttcc atagccgcaa ggcttttcaa
    63421 ttcctgcccg gtctctatca cgccgtcatg tgcaggggca gacaggctgg caagaatccg
    63481 ttcaattttg aaggggactt ctcccggctg caaaggcaga agtgcataat catggacgcc
    63541 ttttcgcatg agaccgacag catcagcaac ctgtgctgcc ggcacgagaa gaagcacagg
    63601 atattgcccg gcaagatcag ccgcatgcgg cagagaatcg gttacagcgg cgtcgcctgc
    63661 aatttccgaa agcgtatggc cttccagatg gagcataact tcgagcgtaa tccgaagggg
    63721 cacgctcgta atgtttaata gcacacaagc catacaattg aacctgtctg atggaaaatt
    63781 tctttattgc tctaagagct tagcaagcct tttcatcttt tcaaggctgt ctgaaagacc
    63841 gcctgagcgg ttgcgggcaa gccaaagttg ttcatagtcc gccaaaatag aatcaagcac
    63901 tcgtgaaaac tcctgacgct gttgggcagg cagccctgat gtggcgcagt tattttccag
    63961 gcgcgctttg cctatggaca gagacagaag cgccaagcgt gcattgagag tgaactcttt
    64021 cttaatcaag gttccgtctt ttctgcgggg atcggacttt gccaggcgct ccagtgcact
    64081 ttgaattgct gaggcggctt tttcaatccc ctgtgctgtg atgccggaaa agaaaccgct
    64141 tttcgggtct ccttcaggtg cataaagcag tcatccgtaa tatacagcgg cattgtcccg
    64201 ggaagtgcct gtctgtagat aagcatttcc aagatcaaag gcagatcgcg ccagcaatcc
    64261 ggaagcatct tcaaagatat gcagatctgc cgctttaacc agatccaatg agcgattggc
    64321 atcataagcc caactcagag cggcaccctg cgcaaaggga aggtaggaaa caggcggaaa
    64381 ctgccagtgc ccgctgtcgc cccagtcggt gataagatag ccgattgcgc cctgattaag
    64441 accgttgcgt gccgcccgtt caagatttgc aagtgcattg tctgtccttc caagcagaga
    64501 attccagctg gaagtgcccg gacaaacata aaaaggaata cctgaccggg caaattgtgc
    64561 gcatctttcg tcgaaaggat gtttcgcctc gtatccccat tccatggcaa taatgttttt
    64621 gggaagttcc ggaattagct caggatgatt aagaatgatg tccccccaaa attgcatctt
    64681 ttttccatgt ccctggacaa gatcatagat ttgggccaga aagttcaggt aaacacgtcc
    64741 ttttccaaga cgttccgcct ctgctttgct tgctccttcg ccaagcgtaa aagtttcgtc
    64801 gcagccgaca ttgacctggc tgctagaaaa gcaggggaga aggctgtcat acatgctttt
    64861 cagcagggca atacttccgg gattggtagg gcaaagatca gatctgccgg gaatttccgc
    64921 aagagaactg tattcagggt gggcaagcca ccgctccata tgaccaaaag agttctggtt
    64981 ggggaccagt tctatgtagc gggcggcaca ataacggtcc aattctctaa tttcatccgg
    65041 tgttacaggc gaggcgtttt tccagacgac ttcatgaccg ggataagcaa aactgtgttc
    65101 tgtgtaaagt tggagctgat tgaatttcaa gtgggcaagc aaatccacat attcgtaaag
    65161 ggtctgcatc gtaggcactt tatcccgggc aatatcaagc agaacaccgc ggttcgggaa
    65221 gtcgggccag tcttcaatgt gcaggcaggg aatgcgaccg gatccggcgt aaagccgttg
    65281 gatctgggcg agggtttgtc ttgcataaaa taatccgggt tcatctgcgg caatgatcgt
    65341 aatatctttt tcggaaatat caagcgtata gccttgctgc ttaagtgtct ttgcgtcaaa
    65401 acggacagca ggttcctttg cgggaaacag gttagagcca ttcaaagaac ggataagacg
    65461 tggctgtggc agaagaacaa gacttcccgg ttcaggtggt tttcggtctt ttacaacagc
    65521 tgttgcatta atatctaccg ggattcttgt ggtttctgtt cgcataacaa aaacctcctc
    65581 ctcttcttcc ggtaaggtgc tgcaggccga cagaaacaca agcatcgaaa cagagagcac
    65641 agactggaga aaatatttca tcgtaaaagt cctttagatg agccagcgga aaaatcaatc
    65701 agaattggaa gagtccggct tttttttctg agcagaagac tcttttatcc ttttctgaaa
    65761 agttgaaata agtaattcgc tgatctgcgc atctttgatg ccttctgcca gcaagtgttg
    65821 cgataaagaa gaaaaatgat ctgccagcag cgtttcaagc cattccaaaa agttgctgcc
    65881 tgcaaaaaag atatttacat tttcgataga gtgattttct gccaaaagtt catctaatga
    65941 ttcctgtatc tcccgttcag tttggatgtt tttggagaga atgagcgcgc aggcatttat
    66001 ttttgtttca ggccgctctg aattggcttc aaaacaattt ccgtataaag catagggcgc
    66061 cagttcgtcg aaagctacaa ggaaaatgcg gtcaggtctg attccatggg caaccgattg
    66121 ttcagagaaa aaatgcaggg ctgttttaag attatccaat aaatttgtcg gctcttggtt
    66181 ttctgttgac tcttgaagta actctgcagg aacgtcacgc tgcagcgaga tggcaacgac
    66241 agcccagccc tggcgcaaac agtttttgaa aaaaagtgca ggaaaagcat cggatgctgt
    66301 tttctttaaa aaaaatacag caggacacgg caggggatgc ccctgaggaa gccaaagatc
    66361 aaagctctgc ccggcttcct ttccgtaaag cagtccctga tagctagggg ttcccagaat
    66421 gacaggttgt gattctgtgt tttcttccaa cgggatggat tgcccgcttt ctgtgtctga
    66481 ctgccatagt ttcgagcgaa atgcccgggc ttcttcgcgg cttaaaatgc catcgccatc
    66541 aagatccgcg tccgggtagc gctggagaaa tcgctgcagc ccttcgcttg aaccctcagc
    66601 ggtggagttc gtatggacag ccacagtcag gatcagtaaa aaaaacggga gaaaaacttt
    66661 acctttcatt gttgcaacct ctacctttat ccaattaaaa aaatgcggct cgcctaaact
    66721 tctcttaaaa aaacatcggc aatcaaccga attttcaaca ggagtttacg aaacaatttg
    66781 tttttttgtg cacgccggcg gaaaaacagg attagtttcc tttccaggct ataacgggtt
    66841 aattttgagc ggggcggttt gcgcagtgtg gtgccctcga tttggacaag aggctgccac
    66901 agattttcaa gcggatagcc gcacagggcg agatcttccg gattcaaagc agcaatcatt
    66961 ttttttgcca gagttaattt caggggatca gcgtgaattt cagcagccca cttcttgacg
    67021 ctggctgtag tcggccggga atgttgagaa acgccgatag gatcaccaag acctcccgtc
    67081 tgttgtttgt cacggtgctc cccgtaattg acggcttctg cctcaaaagg aattcctata
    67141 tgattgcaga tcttttcgaa ccattcttca ggcgctttaa ccagatcttc atagcgtacg
    67201 tgcaatttcc ttgtggacgg attgcggaga aaggcagcca tggcagggat atatcgctcc
    67261 agtacagggt tataggcatg ggcttcctga tagctgccat cgaagaaaga attcgcaaaa
    67321 gaagaaaata ttgccagcgg atgacgggta agcacgatat aggccgcatc cggatagact
    67381 cgggaaagaa aggggagtac aagcgcataa gcaggcgttt tatcgagaca gtacagtttc
    67441 gtgcccttgg cttggagaaa acgcccgtac agtacgtcgc aataggcacg acacgccgca
    67501 taataatcgt tttccccttc cggaagttgt gacacaaaga atttttgcgc ttctgctgcc
    67561 agaatgtggt cgtaaggggc cttatcgact ttgtcccata cgcctgtatg ggcaagagga
    67621 gtgataatgt gcggttccgg accgccctgc actgcggaat gcgatgaaat aatgcgttcc
    67681 aacatggtcg tcccggagcg cggcgctccg ataacaaaaa tcattttagg cgtaagttcc
    67741 attcagtttg tctccggtag ggcatcaggg gatatcctag catatcaagg aacaaaatta
    67801 aatgatatac tgttctttga tacatgtgcg ttgttgtggt gtgatgaata ttccgtgaat
    67861 tgtcgttttg cggaatataa cgatcaggag gaatctctta tgccttttag ccagcgtgtt
    67921 gttgctttag gtatacgtca tccccgggcg attactgcat ctatggttat cagtgctctt
    67981 ttgctgatag ccgctgtagc aatcccgact ctattacccg gttcacttcc cctgcctccc
    68041 gttcgggtgg acacggatcc ggagaatatg ctcagcagaa ccgaaccggt acgtttatac
    68101 catgattcca tgaaaaaggt ctttgacctc aacgaaatta tcgtagttgg ggtagtgaat
    68161 gacagtcacc cggaaggggt ttttaatccg gaaacattgc ggcaggttta tgaattaacc
    68221 gaatatgcaa agaccttgcg cggagaagtg atcggcctgg acgatccaat ggcgggtgtt
    68281 gtcccgatgg atattattgc accttccacc gtggacaaca ttgaacaggg cggtccgggc
    68341 gaagttaaat ttgagtggct catgaaagag ccgcccgcga cagaagaaga agcacttgcc
    68401 gtccgggaca gggcagcaaa aattcccttt ctacagggca cgcttgtttc cgaatcagga
    68461 aaagcgctgg cgatttatct gccgatcact tcgaaagatc tgagttaccg gatttattcc
    68521 aaactgcaga aaaaaatcgc cgaatttccg ggagctgaac aatggcacat cacaggcttg
    68581 cctgttgcca atgatacttt tggtgtggaa atgtttatac agatggcgat ttccgcgccg
    68641 ctggcaatgc tcgtcatttt tctgttgatg ttgtattttt tcagaaaatt tgtgctcatt
    68701 ctggcaccca tgcttctcgc tgtgtatgtc gttgtagtca acatgggttt gctcattgtt
    68761 acgggcaata caatccatat catgagttcc atgattccca tttttatcat gcccatttcc
    68821 atacttgact ccatacacat tctcagcttt ttcttcgaca gataccagaa atttaaagac
    68881 cggcgcaaag ccattgagca tgtaatggat actttatttt ccgccatgct ctatacatcc
    68941 cttacctctg ctgccggctt tgcctctctc gccattacac ccattccacc cgtgcaaatt
    69001 ttcggcattt acgttgcaat cgggattatg attgcctggt tctttaccat gactttcata
    69061 cccgcttaca tcatgcttct gccggaacgc acgctcgcac gattcggcag catgatcagt
    69121 gaagaagatg actcaggtcc tgacacttct accaggttgg gccgccttct ggccggtgca
    69181 ggtgttttta ctttcagaca tgcgcgcatc gttcttgtcg gtgctgttct gctgggcgta
    69241 gtggctgcct atggtatcag taaaatacag gtaaacgaca atcctacccg ctggtttaag
    69301 cacagccacc ctattcgtgt tgccgacagg gttttaaatg aacattttgg cggcacatat
    69361 atggcatatc tcgctttgca gcctgaaagc accggtcctg aactgggcga atatgcagtg
    69421 gatttggagg cacggctgcg tcggacagtt gacgaaatga atctcgacga ggagccgaaa
    69481 aatgccttgc ttgctattgc ctctcttctt cacgaaaagg gagcagaaac gcaaacgatt
    69541 caggagttgc gtcagatgct gcataaggaa gccgatgaca ggctgaatgc ggcgtcggaa
    69601 gcactgatga ctgcggatcc cgaagacagt gtggctgaat ctcttgttga agcctgggaa
    69661 gacctgctcc ttttcctcga tatggaagag cagcgcaatg aaatattcaa gcagccggaa
    69721 gcgcttgctt acttagaaaa actccagaat aagctgcttg aaaccaacgt tgtcggaaaa
    69781 tcgaactctc ttactgatat tgttaaaacg gtttatagag aactgatgga aggaaacgct
    69841 gactatttta ctattcccga aacggcgcgt gctgttgccc agtgccttat tacctttgaa
    69901 agcagtcatc gccctcagga tctgggtcat tttgtttctc atgacttcag agaaacgagt
    69961 ctatgggtgc agctgaagag tggcaataat caggatatga aatatgtgac agaagtcatg
    70021 gacgctttca tcgccgagaa cccgccgcct ttcggtttaa agcaccattg gttcgggctg
    70081 acatacatca acgtggtctg gcaggagaaa atggtgatag gcatgctcaa tgccttcttg
    70141 ggaagttttc tcattgtcct gctcatgatg actgtcctgt ttcgttcagg gctgtgggga
    70201 cttatttcca tgatcccgct gacagtcacc atcgcactta tttacggtgt ggtcggtctt
    70261 atgggcaagg actatgatat gcctgttgct gtactgtcct cccttacact tgggcttgcc
    70321 gttgactttg caattcattt cctttcaaga agccgtgagt accacgactt gaaaggaaac
    70381 tgggagggtg cctatccgct tgtatacgga gaacctgtcc gggcaattac cagaaatgtg
    70441 attgcagttg ctatcggctt taccccgcta ctggctgctc cgctggtacc ttacaataca
    70501 gtcggttttt ttatggcagg cattcttgcg tgtgctggat tatccacctt gttctttctt
    70561 ccggctttga ttaaagtttt ggagaaactg cttttcccgc aggataaaat ctgccggatt
    70621 acctgccgct gcggaacctg tgtggtgacc gctgtcattg caacagcaac tgtagcgcta
    70681 aatgtgcagc agttccttga tatgacctgg acaggtttgt cacttgtgag tctggttgtt
    70741 gtcctggctt ccgggacagt ttgtttcttt atgtcccgcc gtgaatcctg taaaactgtt
    70801 tcagatgaaa aataagcgtt gcgtaccttg cagaaaaaag gagaatgaat catgattaaa
    70861 cacgcaaaaa tgatgctgct tgcaacttgt ctgattactt tgacctgtag tgcactggca
    70921 caggatgccc cgtcagtgga tgaaatcatt aaaaaagcca accatgctgc ttattatcag
    70981 gggagcgatg gcagggcaaa agtgaaaatg agcattaagg ataagcaggg caatgtgcgg
    71041 gaaagagagt tttttatcct gcgtcgcaac gctgatgata aagatgaaga acagcagttt
    71101 tatgtctatt tcaacagccc ggccgatgtg cgcgaaatgg tttttatggt ctggaaacat
    71161 gtgggaaaag atgatgaccg ctggctctat ctccctgctc tggatgctgt ccgtcagatt
    71221 gctgccacag acaaaagaac cagttttgtc ggctccacat tcttttacga agatgtctcc
    71281 ggacgcagta ttgaagaaga cgtgcacgaa ctggtagaaa ctaccgacaa tttctatgtg
    71341 atcaaaaatg tacctaagaa tccgggttcg gttgaattcg attcttataa ggtttatatt
    71401 cacaagacaa cttttatccc tgttaaagta gaatatgaaa aaggcggtaa catttatcgt
    71461 gttggcgagg cgcaaaaagt ggatacaatc gacggctttc cgacagtcac gaaaaatgta
    71521 atgaaggatc tgaattcagg aagtgaaacc acgatagaat acctgaaggt ggattacaac
    71581 gtgggaatcc cggaaaatat ctacacagag cggtttatgc gccgtgcacc acgacaatat
    71641 ctgaactgat aaaaacagcg acttttccgt taccgcctga actgtctaga aaggggtctg
    71701 catgcgtccc ggatatgcct tttgggtgtc atttctgatt gtgctcatga tcgtgagcgt
    71761 ggttgctcag gctgaagagc ctgctttgcc ggccgggctg gagcctgtcg ggaaaagcag
    71821 ttcagaaccc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    71881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagaacccg
    71941 cattgcctgt gggactggaa ccttccttgc ctgccgggtt ggattctgtc ggcgagcgca
    72001 cccaggagcc tgctctccca tcgggactgg agcctgcttt gccggaaggg ctgggagctg
    72061 caccttttaa tgaagaaaac gactccgcct cagctctacg caaggaatcg ccgcttcagt
    72121 ttcatggatt ctgggatgtt cgtgcaggca gcagaataac atcagatccg gcacaggcta
    72181 aagacgcgac actgggcgaa atgcgcctgc agctcaaaac cgataaagac tgggatcggg
    72241 tgtttattga atttacgggc gatgctcttt ttgatgcggt tgaagaacgg gcacgcatag
    72301 acctccgaca gctgcgcctc acctggacac ctgttgaatc gcttgacata agaatagggc
    72361 ggcaggtgtt aacctgggga acaggcgaca tgctcttcat caatgacctg tttccgaaag
    72421 actggcgttc ttatttcagt ggcagagacg tggagtatct caaagcgccg tccaatgcgg
    72481 cccgtatagg ttggttcaat gaaataatga atgttgaagt ggtttacacg ccccgcttca
    72541 atccagacag gtatatcact ggaaagcgtt tcagctattt cagtcctatg cacggaaaaa
    72601 cagtaggcag aaaacaacag gtgcacacca tagaacctga tgactggttt gacaacgatg
    72661 agatcgctgt ccgcctgtac agaaatcttg gcagtgcaca gcttgcttta tacggctact
    72721 caggctactg gaaaagcccg ggcggcatgc agctcttccc gtccagagcc atttttccga
    72781 aattgagggt ctatggtgcc agcatcagag gcgccctcgg gaaagggatt gccaacctgg
    72841 aatttggcta ttatgactcc tatcaggacc gttccggctc cagccccttt ataaacaaca
    72901 gtgagatccg ttttttagct ggctatgaac gtgagatcgg ccatgaattt actgccggaa
    72961 tccagtacta tcttgaatac atgcagcagt ataaagccta taaaaaaacg cttccttttt
    73021 tcatgaaagc aagagatcgg gacagacatc tgatcacgct gcgcctgacc aaacaacttc
    73081 ttcagcagaa cctgattctc tccttgtttg cttacgcaag cccttcagac agcgacagct
    73141 atctgcgccc caatgtcagt tacaaaatca atgacaactg gacagttgat ttcggaggca
    73201 acatctttat gggtcgccag cgccacacgt tcttcgggca attcgaagac aatacaaatg
    73261 tgtatgcagg ggcaagggtg gcgttttaat ttaatccgga tagaggaaaa gaaagggact
    73321 gatgaaaacc ttcgggcgct catcagtccc tgattatttc agagggatcc aggtggatca
    73381 gatcttaatt gttctgcctg tttttgcaga tctgtatgcc gcttctgtta cagcagtggc
    73441 agcatagccg tccatacctg tgctggcgca atcgcagcgg gactggacag catcaacaaa
    73501 gtttttgacc aatgccatat ccggattgtc tccccaggca tcgtatctta cctgctggtt
    73561 gccatcgtcg aagacagtaa ctttctggtt aaaagcgtct actgtaatca atccgccttc
    73621 gcacacaaac tcaagcgtaa catcgcccca ggtcggaaaa cttttcggac ggctccagct
    73681 cgcaagatgc gacacctgga taccgccttc catttcaaga tggaggctgc cgagatcgtc
    73741 agtcggaaaa gatttattgt gcaactggtt gccaagttcg caataaaccg atttaaattc
    73801 cttgccaagc atccagcgaa gaagatctac cacgtgcacg gtgtgatcca tggtggcgcc
    73861 accgccgctt aaagcgggat cggagaacca gccgcccggg tactgaccgt ggttggtgca
    73921 ggtaatagca taaacagcac caaacttacc ggaattgagc atagattttg cttcaatgag
    73981 gggtgtggag aagcggcagg ggaaagctgt acccagtccg acgcctgctt tacggcaggt
    74041 agtgacgatg gactgagcat ctttcattgt gggtgccaga ggcttttcgc agagaatcca
    74101 ttttttagcc cgggcagctg cttctaccat ggcacggtgt ttggaattct ccgatgtgat
    74161 aatcacccct tcgatgtcgg atgcaagaaa ttcattcata tcttttacga agtttgtctt
    74221 gaattccttt gccatttttt tgccgcggct ggcattgtca tcccaaatgg cggacaggga
    74281 aactcccgga agcgctttaa gacaggcggc atagctattg gcgtggagat gcgcaaaact
    74341 cataatacct attttaacca tgattaaaag cctttcctag aatttaaccg gttctttgga
    74401 ggaattggat ttcaaggcgg cctccgccat ctcgacagcg cgcaaagcat cgagcggcgt
    74461 gacccggggg gtgtgaccgc ttcctatggt tgctatgaaa tcctgccatt caagctgata
    74521 cgggctgacg gcaacgggac tcatggggac aaacatggtc gggcccgagc cggtttgccg
    74581 gggttctgcc ccaaggggcg tttccgtgct gtcaaactga atcatgcctt tgctgccgca
    74641 tatttccgcc ttcactttaa agcctgaagg ggcagcccag gtgttgataa tggtggcaat
    74701 gacaccactt ttcatgcgca tggtgacctg tgagtaatcg agctgcgtcg gttcggtgcg
    74761 cataatggtc tggcagaaga tccgttccgg ttcaccgaat acataccgaa cccagtccag
    74821 atcgtgaatc atgcagtcga aggtgacgcc accgctttgc ttgtagtccc tgaaccagct
    74881 gccgggcgca ccggggaaaa tgccgccacg gaacaatttt acccagcccg tttttccgat
    74941 actgcctttc tggatgcgtt cacgcatcag ctcgaattcc tggaaataac gaacgacatg
    75001 cccgacaaac agggttacgc ctgctttttc ggcggcacgt atcgcccgct tgcattcttc
    75061 agaactgcga ccgaaaggct tttcacaaaa aatatgttta cccgccgccg ctgccgccga
    75121 gacaaaagtt tcatgggagg gcgtcggtgt cgtaatcact acgatatcgg tgtctttcgc
    75181 ttcaatcgct ttcatggggt caacaaccgc tttggcgccg aaatcgtctg ccagcccctt
    75241 tgccgctttt ctgaccgcat cagcgcaata agaaatgcgg agtccgcagt tagtggccat
    75301 ttgcgcatgg agccgaccca tgccgccaca gccgagaatt gcaacgttca taaattgttc
    75361 tccttcagtt aagggttaaa gagaaactgt acactatctg agaagccgaa taaaggcact
    75421 atagcgaaat aattgcctcc agagaagtgc gaacatgacg gagcatgact tccgtgctat
    75481 gctggtaagg gccgtactcg gcggtcaaag caccgtcaaa accgatttct ttcagcgctg
    75541 ccatcacttc aggccagttt acatcgcctt ccatcagcat cacaaaacca tcgaaattcc
    75601 cagcggaagc acggaaatct ttggcatgta cggcacagac tctgtgtccc agaatacgca
    75661 aatgctgctc cgggtagccg tacagaataa tattgcctgt atccacatag gcgcccacat
    75721 gttcgctgtc gcattcatcg atgaagtcgc gcatttcaag agggcttagc aggaatttgt
    75781 tccagacatt ttctatcccc agagtaacgc ctgtcgtttc cgcctgtggt acaagtgcca
    75841 gaagattatc cagagcattc tcataggcat catcatagct gagatcagaa gtaacgctgc
    75901 ctggtactac cagcacggac tgcgcaccga gccattgtgc cagctggagc gcctgcgcag
    75961 tttgttctat accttttttt gcgagcgttt cgtcaggtgt tgtaagaggg tacttccaac
    76021 cgattcctgt acccagactc ggcagaacaa gccctaattc ctcagccttt ttccggagta
    76081 ccgccgcttc gctttgcgtg gtgctgagcg gcaacgggcc atcttcgccc acgcataatt
    76141 caaaagactg aaaacccaga cgtgcagcca attccattgc atcaagtgct ggcatatcag
    76201 caggaaaagc ccattgattt acaccggtaa gcatagccat cccttctgta agagaaattt
    76261 ctctgaattc atacaattcc ggtgtatccc gtcgggggaa ccacggaaac actacagcaa
    76321 gacaaacttc cacataaaga ctactaacta tttacggtga aatgcaaaga gacggctctt
    76381 ttttcgcttc agtacccctt ttttctttat gatgagccga tgaaaatgaa tgtcgttact
    76441 acaaaaaaca gtctggtact gtgcctgctt ctggttgcac tggcagcagg ttgcgggctg
    76501 cgcctcctgc atatacagaa tgaatcgata tggtgggatg aatatgcaag ccatgcgcat
    76561 cttgatgcct cttcgttcag cgaattttat gatcttgtgc gcacccttga tcctctggca
    76621 ttgcccggct atctggttcc ggaatatttt tgggctaaat gggtcagccc gtcccttttt
    76681 tctttgcgtt tggagagcat cgcaattgga ctggcaacgc tgcttttgtt atacgctttt
    76741 gccgataagt acctgagcag aagagttgct ctgctttctg tatggctgct tgccctgtcg
    76801 ccggtgcata tccatcattc gcagagtatc cgcatgtacg ggcttctggt ctttcttgct
    76861 cttggtgtca tgtggagttt tcttggtttt ctggagcgcc ggaatctgtc atcgattttg
    76921 ctgcatggcg ttttttcttt tgctctatgc tggacgcatc cttttgccgt tctggtgctg
    76981 gctggtcctg ttacatatat gcttttcacc cggcagaagc aattgcgcct tcttttgatc
    77041 tggtttcttc ttcagggaac aattttcata ccccaggcct tttatcttgc aaaggtgcgg
    77101 ttctggccgc aaaataccac ggccgcctgg tttgccagac ctacctttac tgaattaatt
    77161 gccgatctct ttttcgatga tatcatttcc tttcattggc agctgcgcct gggggaatat
    77221 gcacaaaaac tcgtgttgtt ccgcagtgcc tccgattggt tgcttgccct cgcaattgtt
    77281 ttttttctta ttgccgcgtt tctctttgcc tggaaaaaaa gaaaagaaac aaaacagcag
    77341 ctcgtacttt attgcattct gccatggctg atcctgccgc cgctggcgtt gttctgtgcg
    77401 tcgtggcttc tgcgcccctg catgtttccc aggtatacgg ttcattgcct gattcctctt
    77461 tacctgctgg ttgccgccgg agcaggcata agcaggaata aactcctggt gtcgtttgcc
    77521 gcgctggttc ttctggcggg cacaggcctc caatgggtgt ggacgttacc cgggccccaa
    77581 cgaacgcaat ggcgacagac tgctctcttt cttcaggagc aaagcaaacg ggaagatgtc
    77641 gtgctggtgg aggagttttt atggcgtgat gtgctccagc acaatgcccg gtgggcggct
    77701 caggttgtat tagccctgcc ggtggcagcc gttgccgatc agaaacttct tgccgccgag
    77761 gctgcttttt gcctgtcgcg gcaaggaaaa gcggagagcg ttgtatgggc tgttattcct
    77821 cgggattact ttgatttttc tccccccgca gacttcgaga gttggctttc tgaagcgggt
    77881 ctttcgttcg aacgcttttg cttaagtgcg atcagagata tttacattta taagatcgct
    77941 gctgaaagaa aatcggaaaa cgctctttct ctggaggagt gtttttctct ggatcgttct
    78001 tttggaggag aggagagaga aaaggcactg acccacaggg agattgaatc tgttggtgat
    78061 ctggcccttg aattggcagt ccagggcagc agggaagagg catgcacaat ttttgaggct
    78121 ttgatgcagg aaagcagttt tgcaaaagag atctacagca gtgtagtttc ggcactgcag
    78181 cgggatgagt tctttttaaa acgtattgaa gcggtgcgct ggctttggaa aggctatggg
    78241 gcccgaaaca acagccagac agcctatgcg atttactgtt tttcaaaagc agcacaaaaa
    78301 gattccgcat ccgggctggc tcacctggag gaggcactgg aaagaattcc ccaacgggac
    78361 tatgagtcag ctgcatcggc tttaacaaaa gcacaggcac agccaggaag ttaccgggaa
    78421 ataacaaaaa atctcctgca ggcactcaat tcggggaagc acatttttga gtgttacgaa
    78481 gcggtaaaat cctatagaga agcatcgctt gcagtagaac agggagatac cgccggagca
    78541 aaacaccttc tggaagaatc gctttctctt gatccggcac ttgctctctc acagcttctt
    78601 ctggcatata tccgcagcgt gtatgaagaa ggacagaggg tgcaggaaat gatggaaggc
    78661 tacgcggcat taacaggaaa aagcatcact gaggcatacg ctcatttggc attgctgcag
    78721 gcagaaaact tccatccaga tctgtgcaaa aaaacactgg acaccttgtt tcaggcagat
    78781 ccggatactc aaaaaatata tagaagcctg cataaggcgt gttcagatgg aaaaaattcc
    78841 agacaggcag ctgcctctat ccgtgcaatg tggaacgcct atgaagtgat ggctgcaggt
    78901 gataatgaag cggcagggga tctattcggc gacgctgccc gtcttgattc aactaataca
    78961 ctggcgcttc tggaatgggg gcttctcctt gcagaaaaag gggcttatga cgaggcgctc
    79021 actgctttga tttctgctgc tgatgctgac cctgattacg cccgctttct tgccgaagtt
    79081 attcaggctc ttgagacagg gcaagcacct gaaaagcaca tcgctgccgt tcgtgcctac
    79141 aggcgtggaa tcctggcgca aagtcgcggg gagcttgatg ctgccgaaaa agccttgaag
    79201 gaagcgctgt tcatggacag tacaatgacg gaagcctata cgtctttgac ttttctgctg
    79261 gtcatgcaaa aaaaatatgc cgaggcagag gaaatattaa cacactattt ttccgtagcg
    79321 gatactcccg tatcaggcgc ctacgggctg atgtcgctgc tgcatcttgc agccggcaga
    79381 gaaggtgatg cagccaagtg cgcctttaaa gcacttgccg gggatgccac ttatacagcg
    79441 cagttcggag ttttttttac ggcgctcttc aaagataaaa ataaaagtgc agtccttgaa
    79501 gaaatacaca ggttgaaggg ggaagggatt aacctggaag cgcttttggg tgatcttttg
    79561 gacagttatt ttcagcgccc ctgaagagca gcaaaaaaaa gaccggacaa ctgcatcaat
    79621 cgcaattgcc cggtcgaact tccagaatat cgtcaggaca tggcaagcgc cggttcctgg
    79681 tccagatacg tctccacata ttcacgagtt ccgggagtac tgaagcggcc gacttcggtg
    79741 actgtaacat caccgagatc aagttcctcc tggatatagc gcagtacatc gcgcggatgg
    79801 gtattgccgc acgtaaatac gtccatggca atcaaaccca gatcggcgta ggtgtgcgct
    79861 gagcaatggc tctcatcaag cactacaaca caggtgaatc ccggagggga atcagcaccg
    79921 aatttgtagc ggatctgaga taccacggtt gcgcccgcct tttcggctgc acgtgccatc
    79981 acgttcagca cgcgcttgtc atcaagacaa agctcgcgag caacattgcg acagtcaatt
    80041 aatagatgtc tacccttgtg caactcataa atcctcctaa tgttgagggt tctggaaaag
    80101 gaactggaga agtgcccgca atgcggcaca tacacaactc cagcctgatt gacaagataa
    80161 aagattactc caaaacacgc attgaaaagt tgcggtatat tacaacaatc caaattcaaa
    80221 atcaagtaaa aaagacagta tttttccgcc tggatttttg gtgaaaaaaa agcccggagc
    80281 gggcttcatg cagttgcctt acagcacttc agctgtcggc gctaacaacc acagattaga
    80341 gcgggacatc gaataaaaaa gaaatggtcg gggcgattgg atttgaacca acgatctctt
    80401 gacccccagt caagcgcctt atccgggcta ggccacgccc cgacatgctg aaatttgcag
    80461 tgcccaaatg ataacaaaaa gagcagtgct aaagcaaacg aaaagaatgg gcagggatgc
    80521 agaatttttt cccacacttg gcacagagaa acaggagaag aaaactaacc acgaagagca
    80581 cgaaggctca ggaagaagag aaagagagat acagacttca cgcagcagcc aagtcaacta
    80641 accaatcgcg ccaacacacc ctgtttgcca atgtgtaaac gggcgttctc ttggggctca
    80701 tcc
//
LOCUS       DAOPWB010000002        80699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_254_length_80699_cov_6.052788, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000002 DAOPWB010000000
VERSION     DAOPWB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 80699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 80699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80699
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_254_length_80699_cov_6.052788"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PKN92_00355"
     CDS             complement(<1..297)
                     /locus_tag="PKN92_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47269.1"
                     /translation="MSKKSMLWSAALLAGFVCLGLAAAPVAVADDVEIKIELPEPFFG
                     GTPIDYWSPNLEPEDFKDRPPFMAPKGTALVSKDKPVTSSEAPLHGKLTQITDGD"
     gene            complement(605..2767)
                     /locus_tag="PKN92_00360"
     CDS             complement(605..2767)
                     /locus_tag="PKN92_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNZ47270.1"
                     /translation="MMTNSHEQNRMWLLETLEYLENFSRLDEDYCGDRAYESKAVSYQ
                     HAVVRDGRYRVVFLGAFNVGKSTALNAFLGGSYLPMDIEECTSRLTFIQRGDRMQLLL
                     SLDTAASRHEIEALERALAAVPVIVESEGNPLQISVLCQDQNPDSMRRALEPLITVLA
                     DEAFPHLSSLREKTESLILCLPEEALQDDLVLIDTPGVHSISETRQKITYDIIEHCHL
                     VIAFVDSAFVGNIHDLNFIKRIITCRDRRVFFVLNKADKLENDEIDPRGTFGPAGQLV
                     ETFRRNDIPDHSDIFFLSGYRALSALELDQKRLSFEEALADNKLMLPTSILERLEHSD
                     TPEEDLSAFLMGQSRFPLLKERLMDYLINENKSNAVFTRAARFVYERASDYLITISNT
                     LALARNPEKFIELKENRDLLNNQMATIRTEVDSILVRYNIQFHGGQQDEVFGPGYDAH
                     LRAQLTVPLIESEIIQPLLDWLRKKGQLKEIRQGNFHPLAAQLEHEVDVFITGVIKKL
                     RAVIETNEKRTSEEVSRQISRARELRAALSEPERLVNVAWDAAQSEEKKVFGSAGAGL
                     NTFTGKSISSVIPAVGAAIGVGVGGFMGTIRGWVARFAWSEERWIKKIMPVLRQETHK
                     LLMQETQEESASESVLTMLLNYSQRRGEAFEQAMRKELEHVIETVQQECESLLSREME
                     IRREQDTIIERLKPKEAKLQSIRERAEVMAEEDPETSI"
     gene            complement(2764..3615)
                     /locus_tag="PKN92_00365"
     CDS             complement(2764..3615)
                     /locus_tag="PKN92_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNZ47271.1"
                     /translation="MNNSFSQTAQATPEAAALFVGDFNVGKSSVLNALMRRNLLFTSR
                     EESRTPPVFLTRSGEETIAYSAYNPEEKTFTEQSHTQFLSTRRHGRTKCPFSALSVLV
                     PQFPFTRLTLIDTPGTSTEVAEDTLLPAGKAADKSLIVLVTTVEYWPARHTLALITDY
                     AERFSGRFLVVANMADQLNHGDILRIRDKAAQRIESHSHVKAPPFYIVSAKLEFARRN
                     QDDEYRHRVKREVRDICDAGFDALRVALYEFEAKCQTPEGTCPLDRIVHPLLTALISN
                     NQQEQEA"
     gene            complement(3684..4709)
                     /gene="trpD"
                     /locus_tag="PKN92_00370"
     CDS             complement(3684..4709)
                     /gene="trpD"
                     /locus_tag="PKN92_00370"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNZ47272.1"
                     /translation="MYSIHDLISLLCCGAHLTREQSAEAMDKLMSGETTPAQAAALLI
                     GLRMKGETAEEITGFAQVMRQFATPVQTSRSPLVDTCGTGGDGIGTFNISTTAALVVA
                     GAGVAVAKHGNRAASSRCGSADVLEALGVNIELEADQVGKSIDKLGIGFLFAKSLHLA
                     ARHVAPVRGELKIRTIFNILGPLTNPANADRQVVGVFNSQLAPTMARALLSLGTRHAF
                     VVAGRDGLDELTISGPSYVCEAKNNDIREYLVNPEEVGIPLAAMAEILGGDAQVNAEI
                     TRNVLEGMEGPCRNVVLLNAAPALVAGGLVDTLKEGVERARYSIDSGAALEKLNALVA
                     FTNRKDC"
     gene            5309..6505
                     /locus_tag="PKN92_00375"
     CDS             5309..6505
                     /locus_tag="PKN92_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47273.1"
                     /translation="MSSISRRNFLWSSLATAGLAGLHLSRTAAETTIQGFDETGTTLQ
                     AGAIWQPVSDRKIKVGIVGYGVCRFGAAFGFQDHPNVEIVAVSDLLPDRCAALAKACR
                     CEKSYPSLEDLIKDDEIEAVFIATDAPAHAWHSITAMRHGKHVACAVPAVFGSLEDAD
                     QLYETVKSTGMTYMMFETTAFRNDAYAMRTLYNAGAFGNMLYTEGEYYHYMDTPIDSF
                     RGWRIGLPPQWYPTHSNGYHTCITGGGFTEVSCLGMRSRIDCFDPKNNRYQNPFATEI
                     ALLRTSEGGSARMAVSWDTPGLEGEVGRVRGEKGSMTDLNYQGLANISSVSLEKTPLP
                     PGMEAGGHGGSHGYLCNEFIMALLERRTPLVHIAEALNMTVAGIVAHISALKDGELLK
                     IPQYTL"
     gene            complement(6599..7474)
                     /locus_tag="PKN92_00380"
     CDS             complement(6599..7474)
                     /locus_tag="PKN92_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ47274.1"
                     /translation="MKHESGWKMTRREALAVFGALAASAKMSGTFAHEVSAFKGIALQ
                     MYTLREPAKKDLPGTLKKVRDMGWEYIQWSGMPDLPASEIRAALDNAGLTAVSCHIGI
                     EAFETDFDGQVAFWRTVGNSDLAPGGMMSDCRKDLEAWLRGAARLDAVAARLKETGMR
                     LSYHNHDWEFEKFDNDPRAKIDILMEATSDLCAELDLAWVFLGGADPADYIKKFKNRC
                     PMIHAKDCLETRKLGRRAQFVPLGQGALNWQAIFEAGKEAGVEWYIYEQDNAEKDIFE
                     CAQESFDFLKKSLSL"
     gene            7683..8216
                     /gene="hslV"
                     /locus_tag="PKN92_00385"
     CDS             7683..8216
                     /gene="hslV"
                     /locus_tag="PKN92_00385"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNZ47275.1"
                     /translation="MQEFHGTTILSVRKNGLVAMGGDGQVTIGDTVMKGNAVKVRRLA
                     EGEVLAGFAGAVADAFALFERFEGKLKEYNKNLLRSAVELGKEWRTDKYLRQLNALLA
                     VSNADISLLISGGGEIIEPEDGILAIGSGGSYALAAARALSSHTDMDAREIVERSLEI
                     AADICIYTNRHITIELL"
     gene            complement(8208..10565)
                     /locus_tag="PKN92_00390"
     CDS             complement(8208..10565)
                     /locus_tag="PKN92_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47276.1"
                     /translation="MRVFISCFFLCVVSFFAAADTVYLSSSRAGHSLQAAKTTPVMKT
                     ALRVLSEEIEKRTGSDSTDLSSGDEKVRKICLKHNPSLPAESYKITLSPEELIISGHD
                     GRAMIFGVGHVLSHSHYGKNIVQYCGGAVTLATPAYPMRGHQLGYRYNANSYDSWDDR
                     QYEQYIRELALFGTNSIENIPFQDEIPSPHMPLDRRTMNRRMSEICADYGLDYWIWTP
                     AVFDLADLEKRKAHLAEYKELFNDCPELTGVFFPGGDPGANPPELVLPFLEDLVKPLS
                     RTHPEAKIWLSLQWFTAEQCEDIQRWIDREQPKWFGGLVSGPGSPDVPQTRKRLNKAY
                     PVRHYPDITHTVRCQYPIPWWDHAFALTLGREAINPMPHFYGFVHNYYAPMTVGFITY
                     SDGINDDVNKIVWSRRGWDPEAKVQDILTEYASFFFPVGEETLIADGIAALERNWAGD
                     LESNGGVAATLTLWQDLEKRYPRLQDDWRWQSLLVRAYYDALVRERLLHERALEKEAN
                     AVLLKASKSGADAAMDQALNIVNAAEVPAGISVLREKVVALYDQLFHSIGLQSSVPKY
                     KSRGYERGCSLDFIDYPLSNRWWLEDQFAEIKALSSEEEKLDRLAEIALWESPGSGSF
                     YDAVGQVGKSPHVKRAEAMNTDPEMERCGNPEAWWWDQGFSRQRLTHQSTLSIPFSMM
                     YEGLERDAEYVLRIIGAGEAFPRANGFALVPSAYSTELGGVKEFPIPNAITTGGTLII
                     DWTAIEEEHINWRQQSRIAEVWLLKKGDPAEKVWQQPDGHDDYLE"
     gene            complement(10644..11543)
                     /locus_tag="PKN92_00395"
     CDS             complement(10644..11543)
                     /locus_tag="PKN92_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ47277.1"
                     /translation="MWRFVHITDPHLASERDGVWNNRFLCSMMPEVMACLKRDLQRLQ
                     PEFLIVSGDIVSHNTEEAVLQARDAIESLGFPYYPLGGNHDCYSLESRKWFTDAYAHR
                     LPDSKTYYSFAHKNIRFFALDPWWVHNDGSLSPEADKNAAKRQQTNLNNMRWALPEEQ
                     FLWLIKELERTAEKTACAVSHYPFLPVPDRFRRPGYKFGGNLDNGKKISRELAAFPKM
                     KMVLSGHIHTNSIQRFQGIYHVTTSALPEYPVEFRIVDVYRNRWEIKTQALSDSSFAE
                     RSLRPGHSYTAGTTRDRQATIYL"
     gene            complement(11701..13110)
                     /locus_tag="PKN92_00400"
     CDS             complement(11701..13110)
                     /locus_tag="PKN92_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNZ47278.1"
                     /translation="MIQKLLAKFGFTGESVCKAARDAIQGAQREGWTAKKIEKTLRQT
                     VLPPQKRMRDPLFAPLALALLGKGAGTVRYETVCAPGKELSFAQWGTGIDPAAIRQMK
                     DACSLPISVKGALMPDAHVGYGLPIGGVLATDNAVIPYAVGVDIACRMRLSVLDLPPS
                     LLDDGNDTRLKEAIEQETRFGIGASFKKKRLHKVLDENWSVSPVTRKMKDTAWEQLGT
                     SGSGNHFVEFGILDVFEDCLGVNLGSYLALLSHSGSRGTGAEVCKYYSARAQSSCPGL
                     PGELSRLAWLDLKQTEGREYWDAMELMGRYAAANHELIHDHITERLGAQVLLKVENHH
                     NFAWKEMHEGRQLIVHRKGATPAWEGQAGIIPGSMAAPAYVVHGLGNPLSLCSAAHGA
                     GRNMSRTQAKKLFSWHQLNDLLKKDRITLLSAGLDESPMAYKNIDEVMAAQRDLASIK
                     ARFFPRLVKMAPAGERPED"
     gene            complement(13107..13631)
                     /locus_tag="PKN92_00405"
     CDS             complement(13107..13631)
                     /locus_tag="PKN92_00405"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ47279.1"
                     /translation="MPNGLPLVPFEWHYCATCGTPLVDEHDGESVRPYCPPCNRFFYR
                     NPVPACCLFIRDEKNRLLFGRRNVEPCLGKWALPGGFMELLENGEQCAVREMLEETGL
                     EGSGPVLLGAHSSRSADKGSVLVLGYVITAWSGDLSPSSDVSELCFFSRDERPDIPFD
                     AHRSLLALYDTLYP"
     gene            complement(13777..15117)
                     /locus_tag="PKN92_00410"
     CDS             complement(13777..15117)
                     /locus_tag="PKN92_00410"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HNZ47280.1"
                     /translation="MHIESSKKKWHPYSWQEKPALQQPSYGDKTQVAEVLEILHAKPP
                     LVSRGEIDRLLNELAEAAAGKRFLLHGGDCAERFSDCSAEALRRKLEVLLQMSLVLTY
                     ATRRPVIRIGRLAGQYAKPRSADEEIIDGRSLPVYRGDLINDFSPDEKARKADPCRMI
                     EGYHHAAASLNYLRALIEGGFADLHHPEKWGAHFLHQCADNQDYRRILDAIADAVRFM
                     DMLGGDAADRLKRIAFYTSHEALLLPYDAALTRQENEEHFYNLGAHYLWLGYRTSQLK
                     GAHVEYLRGISNPVGIKVGAASDTEELVSLLQCLNPAHVPGRITVITRFGAGKVKALL
                     PPLINAIRKAHLPVLWCCDPMHGNTESLADGRKTRHVHNIFSELEESFAVHASLDSCL
                     GGVHFEQTGEEVTECLGGNVTQHDLEAHYETACDPRLNGAQALEMAFLIARLLR"
     gene            15329..19369
                     /locus_tag="PKN92_00415"
     CDS             15329..19369
                     /locus_tag="PKN92_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47281.1"
                     /translation="MNPAKCSLKGSAALFLLILSIFHGCPTPASAAVNIARIKPGVHL
                     TDDVLLPDQICCMRVSVTGEASLSDMILTPPPGVYYFHAGDHVILSVFDSLHHTVPIF
                     WDGAGKEATCFKVLTLQEHLEIELSFDPELPVFKETPPDSREQWFDVPTDSTIYAFVG
                     GGNLYGWFTGYRHKDHGAGITGLVHTHYPWMPVFDRTNLNFEHIFSSGAENIERVFFT
                     PRTDPMRFCIEAPNKIRVRWDKESSAWGVDCEMIYCFSGEDAIDISFSFTPEDYHASL
                     DYLGFMWASYMHNVKERGISFFSKTPSGMKWILFAPENDAHKKNGNVVPVEGLESKSP
                     ENAEPALLLKCTDTLTALVPFYYGTFYPDTNLPESFGTMQYVMLFQKSEGLRYAVYNW
                     GPVKHCSTWDWQYLVKSPQAGQRYDYLMRCLITPVITEDEIDLERKVWLKSLSGDSSE
                     EKPLFLFPDLPLYWSPASVFLGLAFMGQQIEPWAPDHALTWYRYLLQESENKQPFSMD
                     IDRYYVRSSNNDGLIHEWETWIEYDNSDPVFWQHLAMAYNTKGNFEKADHCFSEALLR
                     KKDDPHILLHYGINNIYIKKIEYGFNQIKEALKLEPLLESAIPTYLNQCGEWLFEQND
                     LITAIQFYKESARLFPEHYWYIVHLAECYEAMGDDRLAAELFVNMLLKFPESPYSAFK
                     LDRILKDKNDLKERINIWQEIYRQHKNSFIPALYLGKALYHKQAYQEALLIVEQALEV
                     MPDAPEALLYAGLCCLLLNNYDQGYQKIKEALKNDPVLSTEAGTELAAAGDALYKQND
                     FGRALQLFQLAEEFESTNLWHQVRQGECLEAQHKFSDAAALYTAVLLKAPESPYSAFK
                     LDRILKDKNDLKERINIWQEIYRQHKNSFIPALYLGKALYHKQAYQEALLIVEQALEV
                     MPDAPEALLYAGLCCLLLNNYDQGYQKIKEALKNDPVLSTEAGTELAAAGDALYKQND
                     FGRALQLFQLAEEFESTNLWHQVRQGECLEAQHKFSDAAALYTAVLLKAPESPYSAFK
                     LDSMLQDHEDNKVRIKSWQHIYEHTGKASLPAFYLGMSLFIDCHYDQAYALLSAADSA
                     SEENSRAVLYKGLSQVILGEVHEGTENIEAALKQDNTLSHEAGERLSVLGHHAFNEGK
                     YKEAVRWYQQAYLYEPDALWHLYHEAKSLVKCNARSDALTIYKTILEKAPESPESAQE
                     MDTLLIEMEEPAKRVELWRILHGKAEKALVPILYYAAALCDTGEEEQAIELITDFFHF
                     RDTSGFSTDTLPDNDGSNPLPEENAVLDKGKTLVIEQLLHFSHLAKRTGHNELAFFLQ
                     HKADQGRTGQGDSTLSLLEWLNQLRE"
     gene            complement(19373..20677)
                     /locus_tag="PKN92_00420"
     CDS             complement(19373..20677)
                     /locus_tag="PKN92_00420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ47282.1"
                     /translation="MFVAINVVSWQNIIGPVMILFGRKIQMPESLIGLLLSFMPFTSL
                     LLLFTLPMLIRIGPKRVMMTSWFLRNIITCLVFLMPLALASQVKWLSWAVLLVSILGF
                     CVMRALGAGGWLPWLHEIVPTEKRSTYFSTETAMTQLINVAVLFLQARLLAGNPDIPR
                     FLLIYGIGIAMGIVSLITMGRIPGGEGTVEINALTGMSAYKKAACDPVFRRFLLISTF
                     AFSGLVWFSASYVLYLRDVIGLTDSLNMTLTACSGLGIFLTIGPWGRFTDKNGSGLAM
                     AKTMFAHSFAPLILLFLLPGMKGGTAAALAAVVLAGVFNAPFNVAANRAMLNLVPDES
                     RIGYTSLWTICTALALGITPLVAGFLIEHFGLLGFRLCFILSGLSSFMASLLCLLFIH
                     GKAGDRSWLHALVYTVSLPMLGKIFWITMGLDKSNRKEETEI"
     gene            complement(20817..21581)
                     /locus_tag="PKN92_00425"
     CDS             complement(20817..21581)
                     /locus_tag="PKN92_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNZ47283.1"
                     /translation="MDSPKNYLIDMDGVLVKGRLPVPGAQAFVNRLKEKEAKFLVLTN
                     NPMFSPADLAYRLRASGLDIPVENIFTSAMATAQFLHGQRPYGTAFVIGESGLTSALH
                     GIGYIITDHHPDYVVLGETMAYNLEQITKAVRLVAEGSRFIATNPDASGPTESGIVPA
                     CGAMAALIEKATGRAPFFVGKPAPFMMRSAMNYLGVHSQNTVMVGDRMDTDIIAGMQA
                     GMETILVLSGVTRREELSAFPFMPTQVLESVADIKV"
     gene            complement(21647..22669)
                     /locus_tag="PKN92_00430"
     CDS             complement(21647..22669)
                     /locus_tag="PKN92_00430"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNZ47284.1"
                     /translation="MDMIELRSDTFTCPSPAMRKAMADAEVGDDVYGEDPTVRALESK
                     AAELIGKESALFLPSGTMANLVAFLAQTRPGDTVILSESAHPFHYENANLAMIGGLLV
                     RTIPDPLGKFSAEQVQEQIVSEDDPHLSKTTMVSVENTTNRGGGACWDYHELEAVFRV
                     CQESSMRLHCDGARIFHACIETNIQARYYGRCCDTLSFCLSKGLGAPAGSLLTGSTEV
                     IKKALRFRKMLGGGMRQAGVLAAAGLYALEHHLSDLAEDHRRARECRRALENEGVVFA
                     LPSPTNILYIQTANAALKVKQLEEAGVRALAYRDDRIRVVFHRDISDALLSRAIEVFK
                     KILVPA"
     gene            complement(22685..23239)
                     /locus_tag="PKN92_00435"
     CDS             complement(22685..23239)
                     /locus_tag="PKN92_00435"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNZ47285.1"
                     /translation="MRKSGYLLLALLVSLTACARLTNPFSTMRADYTNVPEASLNAFT
                     LAVEQSVLEGNRTSTFDGYDGVASSNEEILQAIHTRAARSELIQALLNSGHAYEQAQG
                     TICILRTRDYKLQTSKKQRDQNALLVMSENANRWTLYEGLCKASNWQRKSLGAVQESF
                     FQARVQSMSAGQKYETADGKIAVK"
     gene            23682..26087
                     /locus_tag="PKN92_00440"
     CDS             23682..26087
                     /locus_tag="PKN92_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ47286.1"
                     /translation="MTSETHIEIARIALFSGDRKESKLLSDELTTLRPAWQIITRPQL
                     KDFDWTHIQSEIDCLLVKADSEKKALSLLEAITGHKKAELPPVIVVTSLSKKQKVREA
                     VNSFNLSRLYVLLDDSDTIISALESIMRAYRVEQKLRQVNTLLSNLIEERSKTLHEFS
                     ERIRFLTEFSSDAVFEIEKDRTTGISTFISANRTACSWLGYSQEELLQLPFSHFLEPA
                     AITNITQLLNTISQPQEFSLELSLKNRNQERLFLNIVIRAFESDRGLRMIAIAHKILS
                     PEFSLSEHASESDYEQVVSHIGQLMYIGNVDTQQMKWSGAASQITGYTIKHLNKGSMR
                     EWRKRIVSEDYPRTIAAINKAILRISSYEVEYGFLHKNGEIRYLEDRGVVLPDHRGAP
                     HRIMGTIKDITEKKMEEEARQHLEKELQHTQRLESLGVLAGGIAHDFNNILAGIIGLT
                     DLALREIPSDSFAFEDLNEVLHAAHRAKELVQQILAFSRQGDHERSLIFPHIIAREVI
                     RLLRASLPPAIEIIDSIDIQSGAVMANASQLHQVITNFSTNAAQSIKGGSGKVEIQVC
                     DVEVENSFSAKNPNIPPGSYVRIAVSDTGHGMTPSILARVFDPFFTTKGPGEGTGMGL
                     AVAHGIITDHGGVILAQSRLKKGSTFEAYLPKITDPQITESLVQSGSADGRNNTHILF
                     VDDDETVLRFASSALPRLGFKITLCKNAEEALHAFAENTDTFDLVITDQRMPGMSGTE
                     LAAAIRKSSPRMPVILFTGFSKTLTEKDQKITGIQTVLRKPIIVRDLLTAIRKTLKSP
                     TDL"
     gene            26130..27224
                     /gene="murG"
                     /locus_tag="PKN92_00445"
     CDS             26130..27224
                     /gene="murG"
                     /locus_tag="PKN92_00445"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNZ47287.1"
                     /translation="MITGGGTGGHTSPALAVIEELRNRDPRLLLQWVGKKGSIEERIA
                     KREGIPFRSIPMEGWTRKISLRLIWVGLKFLYSVLKAWTQIRVFRPDAVFGVGGYVSL
                     PVLLVAPRIGIPAFSHEQNKRLGLANRLSAKKLTTLFLSYEDTLGVPPETPTKVTGNP
                     VRSAFYNPSGKEECREALGLDKEVPVILVVGGSQGAQTLNRAMQEAVFNFSANDAQFI
                     WSTGPGDLADCQALAEKAPCTVQVYPYIENMAQTCGAADLLISRAGASTTAEIAIMGK
                     ASILVPYPYATDNHQEHNARAFEAAGAAQVLLDKNCTGESLTQQIKAIIQNPSVLASM
                     GKAAADLARPAAADDIVETILEKACTPADY"
     gene            complement(27221..27826)
                     /locus_tag="PKN92_00450"
     CDS             complement(27221..27826)
                     /locus_tag="PKN92_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ47288.1"
                     /translation="MHIKQAIEERRAFKKFDKDFQMSEEMIQDLFSSVRLSPTAFNIQ
                     HWRFVLVSDPQLRQELRKVAWDQVQVTDSSLLIILCADVKAWEKEPHRYWRNSSSEYQ
                     DIILPAIDAYYRGKDQVQRDEAMRSCGMAAQTLMLTAKSMGLGSCPMVGFDFEAVGRL
                     INLPHDHVIALMIAVGKTIAPANPRGGHLPLEEILVRDRFA"
     gene            complement(27904..28761)
                     /locus_tag="PKN92_00455"
     CDS             complement(27904..28761)
                     /locus_tag="PKN92_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47289.1"
                     /translation="MEYMEMLDRFSSRSKEKSAESIPTPSIYKKERQKVVVRMKSGDV
                     YYGSTFALNRQAEIFHLELVTAEGISLNKTLPIAFKNVKALFYVKSFDGRFDPAIEFP
                     QDIPPAKALAVEFSDGEVIVGRPMHARWNEEVRFFLVPEDQKSNNLMVLVERSAVVSI
                     QDADLYTREQENAYQKYKATHFKPEMSDLECQGDYLFRSGKYGDAFRIYRDLFKNDQE
                     NSRLKKKVCAARYNLGIRLIRQRNYTKALQFMEAVLALDPSHQQAQSRVEQLRTHIQK
                     KQSQSSRSE"
     gene            28943..29548
                     /locus_tag="PKN92_00460"
     CDS             28943..29548
                     /locus_tag="PKN92_00460"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNZ47290.1"
                     /translation="MQNAYVLVLNKSWVAIQVTSARRALRLLVQGRACVVHPTDYMLY
                     DMPSWLELSMHADGIPPDRYVYTTAQRIRLPEVVVLNVFNGFHVQEVRLSRRNLFARD
                     KDTCQYCGRTGNRSCMTIDHVIPKSRGGEDSWSNLVVACPACNLKKRDRTPDEARMPL
                     LKKPVAPRWLPRFTRPLLREELISWQRFVDLAYWGSDYYFS"
     gene            29588..30511
                     /locus_tag="PKN92_00465"
     CDS             29588..30511
                     /locus_tag="PKN92_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ47291.1"
                     /translation="MDFIRYLHYLRIERNFSVHTLRAYANDIATFCDFIEHGVAALHR
                     KSDDIRPPASLSVLQRADRNTVRAFLAHIQTSGATARTAARKLAALRSVYLYYQKTGQ
                     ITTNPTEGLRSPKRAQNLPEVLSIPEITALLESVDTATALGSRDRAVLEVLYSCGLRA
                     AELAGLTFEDIFWHDETMRIFGKRKKERLVPVGGPALRALKSYLGRRGELCSQAQPIV
                     FVNHRGGALTTRSIQRIVEAHVQKSLPERVGVSPHTLRHSFATHMLNAGADLRVVQEI
                     LGHESLSSTQIYTHVSIDRLKEVYRNAHPHA"
     gene            complement(30533..30946)
                     /locus_tag="PKN92_00470"
     CDS             complement(30533..30946)
                     /locus_tag="PKN92_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNZ47292.1"
                     /translation="MKFETEYLVFQTTKKREYINITDKVEQALHRSGIQEGMILVSAM
                     HITAGVYVNDAESGLIADIDEWLESLAPFKEGYRHHRTGETNGDAHLKSLLVHHEVIV
                     PVTKGRLDLGPWQQIYYAEFDGRRPKRLIIKAMGE"
     gene            31284..32447
                     /locus_tag="PKN92_00475"
     CDS             31284..32447
                     /locus_tag="PKN92_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47293.1"
                     /translation="MAKKTVNVAMIGASFMGKAHSNAWRKVDMFFDAPVKPVMKVMCG
                     KFEEELATADRWGWQETSLDWKEVVNRPDIDIIDICTPNFLHPIIAIEAAKAGKHIVC
                     EKPLANTLAEAKDMLKAADKAGIKHMCGFSYRFAPAVQTMKKMISSGALGEIFHFRAA
                     YLQDWIVDPDFPMVWRMKKKHTGSGALGDIGAHITDLCHFLVGPVGEVTSALETFIKK
                     RVVADSDVGAWDAKGNKKTKQYDTVDVDDAAVFIGRIKGQNTLASFEATRFAPGRRNF
                     NSIEIYGSKGSLIWNQEDMNEFQYFNRADPDTLQGFRKVLACDSGHPYVNSWWPPGHI
                     IGYEHLFVHEIYEFLCGLKKKKINYPTFEDAVNCQAVLEAVEKAASTRKWEKV"
     gene            32510..33472
                     /locus_tag="PKN92_00480"
     CDS             32510..33472
                     /locus_tag="PKN92_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ47294.1"
                     /translation="MKLGVLTALFSDKPLKEVLDLIRPLELQTVELGTGNYPGEPHCP
                     LQELLESKPKRDELLATLKGEGLEISALSCHGNPIHPDEAFAKANHAVTMNTIKLANL
                     LGVGVVNGFSGCPGSGPGDKKPNWVTCAWPPDYLEILDWQWNEVVLPYWSKMNTELGN
                     AGVKFAFEMHPGFVVYNTDTMLRVRRECGDNIGANFDPSHLFWQGMEPVECVKALKGC
                     IYHVHAKDSIVHQTNAAVNGVNDYKSYGDEINRSWIFRTCGYGHDLKWWNDFFSTLRM
                     VGYDGAISIEHEDSLMSNWEGLTKAVSFLKKSIIFEAPTAMTWA"
     gene            33552..33953
                     /locus_tag="PKN92_00485"
     CDS             33552..33953
                     /locus_tag="PKN92_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47295.1"
                     /translation="MKIYLLAIPLIAAVSTLFYLIFREMARAWLHHRLRMLLLEWAEK
                     KPGFSMSSEELRELLEEMPAPSIQTPKTDLTVTGLYLALIGLFFVIAYALIGKSQWAV
                     GAYFGGVACVVLGFILTTIGIILRFLRKPPL"
     gene            33993..35324
                     /locus_tag="PKN92_00490"
     CDS             33993..35324
                     /locus_tag="PKN92_00490"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ47296.1"
                     /translation="MMDTSVHRHRYSGDACKEPLRVAVIGSGPAGFFCTDYLLRLAQP
                     CVVHMYERWPVPYGSARHAIAPDRQRIRAVTTAFDRIASRQGFHFFGNVTVGKDISLQ
                     LLQQHYHAVILATGADDGLVLNIPGEEVPGFFHITKFAGWYNGRPDCAFLRPDLTHTA
                     AAVIGAGNAALDAVRILSLPSDWLPKTDIPEPMQQALASSAIREIHLIIRRGPLDVRF
                     SLSEFAALFDIPGVSYHLHPENCLENIQAVSPEEEQLLSLYKSAHTTPKTARHIHFHF
                     YSTPKEIQGKEAVSALVVKSGEEDTAIPCYLIISAIGQGATEIDGIALNAQKSMIPHK
                     AGRVTQQGIIVPGLYTAGWAKRSGTGMVGKNKPDCRETVQAIMDDRDSLQCKIEAVPD
                     LPKDAVTLDGWHRIDAAERGRAENTTKTRECFLTVEEMLQAAGIKSKLPSL"
     gene            complement(35325..36764)
                     /locus_tag="PKN92_00495"
     CDS             complement(35325..36764)
                     /locus_tag="PKN92_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ47297.1"
                     /translation="MRTIPKVFALFICLCVSFAFADTHSLLILHTNDIHGHVKADPLG
                     QGGLPYVSAFVKEMRAQRGDLLLLDGGDVMRKGDLIAEKTEHRILYEAMKKIGYTAGA
                     MGNHDNFHGYDHLRECSEIAGMPILCLNSLDDTGSPRFAPSTIVECNGLKVALIGLTT
                     LAGGAFKSIDKCGEQLRLEAERLENQIDLTVAVVHIGRKECILLSEKAPAIDVFVSGH
                     THEALHEAALVPETGALIVQAGQYAQYVGVLDLTVDPAQKKAITAESRLVSMDPETVT
                     PDQEMIAWLDAEEERIFPGAGEIVGETGQVIQIPDLVRIASAALQWKAGADIAFCHTF
                     VMRSNQREGAITANSIFQSAGQRGDQVVLTELSGEQIRVYLNCLSNARQGKTAWYGFS
                     GKMEKADEAGKWNLVSDLEMAKNYQVAIPLYQWENLMGECAEPDSPLATLPKTPPIAD
                     YSVADAMTDYLREVKKSGGSVEALLATLN"
     gene            complement(36786..37361)
                     /gene="pyrE"
                     /locus_tag="PKN92_00500"
     CDS             complement(36786..37361)
                     /gene="pyrE"
                     /locus_tag="PKN92_00500"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNZ47298.1"
                     /translation="MNPQEIIRIFKESGALLEGHFIYASGRHGRQFLQAARVFQYPEY
                     GEAFGQALAAAFQDKDIDLVVGPATGGIVIAYETARHLKCRAAYSEKDGAGGMAMKRG
                     FKIKEGERVLVVEDIVTTGGSVKKTIDHLRRRGAEIAGVAVLIDRSSGLASFDCPFHP
                     LAVLPMESWPADALPEDLRTTEPMDPDDIML"
     gene            complement(37358..38044)
                     /gene="pyrF"
                     /locus_tag="PKN92_00505"
     CDS             complement(37358..38044)
                     /gene="pyrF"
                     /locus_tag="PKN92_00505"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNZ47299.1"
                     /translation="MATELIVVLDMDSRAEALSAVHACGDCKWFKIGAQLFTRLGPDI
                     VKEVQQLGKNVFLDLKYHDIPNTVGHAARAAADLGAGLITVHASGGCAMIEAARTAVE
                     GTATRILAVTVLTSINASVLCEEVGFSETPEEAVRRLASQAVKAGAHGIVCSPLELGI
                     TREVLGAEPLIVTPGVRPEWASRDDQARVMTPRAAAQAGASMVVVGRPILKHPYPSEA
                     VSLILEELNS"
     gene            complement(38190..38981)
                     /locus_tag="PKN92_00510"
     CDS             complement(38190..38981)
                     /locus_tag="PKN92_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47300.1"
                     /translation="MTLTREQLTGRLVLNAVLPVIRVMLEDDERMKDRFADVTGTVQF
                     EADDPDGKTGAWLLFDRGKFEVTQGFTENPDVLFSFSSLKAMNDMFRGKPVLPRLGAL
                     LPALMKKPGLVIRVFKVLFGLKLLMPAAKPKNAEQAHLKVKMTLYMVSTALSQMNKAG
                     DTDMQAWTAKQPERIYQWSVDGTDIVCYLKVKAGKTKAGRGVHTGRKPFVHMSFKSVD
                     AALPVLANTIDTVSAIGQGLVLSDGSPEYGGKLGDFMMKVANLLS"
     gene            complement(38985..40001)
                     /locus_tag="PKN92_00515"
     CDS             complement(38985..40001)
                     /locus_tag="PKN92_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ47301.1"
                     /translation="METASTLTWTIYDADKMTQREIYGLVLTDLGRQHPKIVGLSADL
                     AKSTKIGTFGDAYPDRFFNFGIAEQNMFGAAAGLAKAGLIPFVSTMSAFASMRACEFV
                     RTDICYQNLNVKIIATHGGMSFGSGGSTHHANEDLSIIRAFPNLTVVAPADTIETALA
                     VKKCMDIQGPVYIRIGRGFEPRIYESEEYGFEIGKAHELRSGTDITIIATGNTVFHAV
                     QAADIMNKNSGLSVRVLNMHTLKPIDQDAVLKALVETRRLLTVEDHSVIGGLGSAVAD
                     VIAASGKGCAFNKLGVPDTFCPHGNPEDLMHMYKIDTDGIIETVGELMQRDFEEDEDW
                     EDEV"
     gene            complement(40006..40839)
                     /locus_tag="PKN92_00520"
     CDS             complement(40006..40839)
                     /locus_tag="PKN92_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNZ47302.1"
                     /translation="MSLTEQELATLKERAASIRCQIADVTGWSGGAHIGGSLSMVDIL
                     TLLYWKYLRIDPANPAWEDRDRFVLSKGHGGVGHAVVLAERGYFDKAALHEFNETGSM
                     LGMHLDSNKVPGCDASTGSMGHGLSQAVGMALGARVLKKEWRVFCMIGDGESNEGSIW
                     EAAMAAAHYKLDNLIVIQDRNHLMIDGPTEEVMSLEPLPDKWRAFGFETREVNGHDLP
                     ALAEAFEFAIAHKGSPVMLVCDTIKGKGVEFMEGKPEWHYGGLNAEQIAKAKASIENK
                     G"
     gene            complement(40861..42105)
                     /locus_tag="PKN92_00525"
     CDS             complement(40861..42105)
                     /locus_tag="PKN92_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ47303.1"
                     /translation="MNIYRYEESNKWFDRAVKVIPCGVYGHFSPAPYAPVEAYPFFSN
                     KADAARFWDVDGNEFIDYMCAYGPMVLGYNHPAVEAAFQEQHSLSDANSVASPRMVEL
                     AELLCDLVPVAEWAFFARNGADVTNLAVMVARAATGRDKVIAIKGGYHGTSPWMQAPG
                     HHGTIADDHQHVIRIPWNDLNALDQVLAEHGSQVAAFMASPYHHPIFEDNVLPAEGYW
                     QAVIDKLHKNGSVVIVDDVRAGFRLHLGGSNEHYGFKPDMIAFSKAIANGYALSALVG
                     TDALRLDAAKVFHTGSFWYCAGSFAAAIACLTTLKAEKGPERMRVLGEKLTSGLRDVA
                     AGHGYTLVVSGEPAMPYLRLADDDTLMLQQAWCGECTRRGAFFTSHHNWFLSTAHTDG
                     DIEKTLQIADDAFKVIKSEFGH"
     gene            complement(42127..42888)
                     /locus_tag="PKN92_00530"
     CDS             complement(42127..42888)
                     /locus_tag="PKN92_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47304.1"
                     /translation="MIDQLKARLNLKAVLRNVELLPEIDDLSRELIKDWEISIRLSIW
                     RDTNILLNFHNGRCTFLADTIEKGTVSLFFCSAAHLNNMMENNASPLPLSGFTRLGFL
                     KTEFSKLTSRLEYFLKPPSDVVQDDRFKMINLSLALSTAVFAIPELLQLDPVSRRIAP
                     QLSAGTLQLSVLPEGPYAHVIYDGKGGAKAFTGKAAHPDALLSFRNPEIAQLLVDGTL
                     DSFGAVAKGDIQLRGKIPLIDHTNVILDQIPKYLQ"
     gene            43126..44658
                     /gene="xylB"
                     /locus_tag="PKN92_00535"
     CDS             43126..44658
                     /gene="xylB"
                     /locus_tag="PKN92_00535"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNZ47305.1"
                     /translation="MAELLIGLDVGTSGVKALLMDTCGNVVASALREYPLSTPKPNWA
                     EQNPSDWAEASLSALQELAACVPSGSEVKAIGLTGQMHGSVFLDKHNEVLRPALLWCD
                     QRTAAQCQEITDRAGGEAALVNMVSNPALTGFTAPKILWLRDHEPHHFEKLCKVLLPK
                     DYIRWVLTGEFATDVSDASGTLLFDVRNRCWHKELLSLLELDSSLLPESFEGPEVTGT
                     LSSQAAQRTGLSKGIPVVAGGGDQAAGGVGCGIVKAGTLSSALGTSGVVFAFSEEICV
                     DPQGRIHTFCHAVPGKWHVMGVMLSAGGALQWFRNQLCHEEIQKARELDIDPYTLITE
                     AAATISPGAEGLLFLPYLSGERTPHKDPYAKGTFAGLSLRHTKAHMARAILEGVAYGM
                     RESLDIMREIGVPLSVSRISGGGARSALWRQILADTGRVAIARLEVDQGPSYGAAILA
                     GVGARLFTSVEEACDLCIHEKDIQMPDPSVADFYDTYVAEYRAVYLALKSHFKRMAAL
                     VG"
     gene            complement(45459..46463)
                     /locus_tag="PKN92_00540"
     CDS             complement(45459..46463)
                     /locus_tag="PKN92_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNZ47306.1"
                     /translation="MDQRDDLLTILGTEIPAAYHLGAIRPLRYLENTHQRRHKKIVVE
                     SEKGKYLAKTYTNDAVTLDSLYFQHNLSNFLYSHDLPVAQILRTHAGKSFIAKDNWAV
                     ELQSFLSGGPIPLTVQTLKVAGSCLGKFHSVCRGLPSPPRDARKWRFSEVPRAIFQDF
                     YQRVLQERNDAQMHGFCDTIVAFLGEATEELAQEKRDTFETGIIHGDWHGGNLLFVNE
                     EIQGIIDLEYAGDGCYLEDLSYGLSNLCLRASVDAQKLENRTDIYLAAYEQHRSLSWG
                     ERVALFYAAGIKHITTVAFQHPAADGKVAGATPVQWLATLAEQTKWLARQAHNARWGN
                     "
     gene            46917..47684
                     /locus_tag="PKN92_00545"
     CDS             46917..47684
                     /locus_tag="PKN92_00545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ47307.1"
                     /translation="MSSLTVLTLVPGECERLPKALESVSWADVLFCVIDSRAPQETID
                     LVRKYTDTAPVHEYVNAAAQRNWALPQITTEWTLVLDADEWVSDALAERIQAIIKNPD
                     SADGYNILRHSYFLGKLIRYCGWHRDYNLRLFRTAKGHYLDKRVHSKVIVEGRVEKIR
                     DVMYHNTYRSFEEYFSTFQRFTTWGAQDAYERGKRAGLRDIILRPVHRFLKMYFWNRG
                     FLDGYHGAVLCGLSAFSVFTKYAKLWKIQTDAQKPEN"
     gene            47704..48111
                     /locus_tag="PKN92_00550"
     CDS             47704..48111
                     /locus_tag="PKN92_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNZ47308.1"
                     /translation="MTQTLSPMHDDLPDKVYSVSETAQLVGISPHLLRQWEKRFPTEV
                     KPRRNSANHRMYSREDIISLMRIKTLLYHNGMTAKGARQQLRQERFTDGQPRDISGMA
                     RIIDAIADESRAILNLFDPKVDELIPDQDDAFH"
     gene            48258..49712
                     /locus_tag="PKN92_00555"
     CDS             48258..49712
                     /locus_tag="PKN92_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNZ47309.1"
                     /translation="MGGFFAVTSEKDCIFDLFYGTDYHSHLGTCRGGLAVLDGTDFRR
                     HIHDISNAPFRTKFEKDILAMRGNSGIGVISDMEPQPLIISSHLGRYAIATVSRVSNL
                     DFLVQDAFNRRVAHFSETGSIGINPTELIATLINEKTSFEAGISYAQERVEGSCSILL
                     LTEKGIYVARDRFGRTPIITASKEGAVAATLENAVFPNLDYTGQYYLGPGEILRLTPE
                     GIEQLRKPDAYKQVCAFLWVYYGFPASSYENINVESCRYRCGARLAEKDTVEADLVAG
                     IPESGTGYAIGYANAKTVPYGRPFTKYTTTWPRSFMSPGQTKRKLVARMKLIPVPDLV
                     QAKRLLFCDDSIVRGVQLADTIKRLYEAGAREVHMRPACPPLLFACKYLNFSRSKSEM
                     DLIARRTVRELEGGANGSLEEYTNPDSEKYKAMVDRIRQRLCLTTLKYQRLDDLIAAI
                     GLPREDLCTYCWDGCEGQCGRQMTFANGIQETKD"
     gene            49741..51099
                     /locus_tag="PKN92_00560"
     CDS             49741..51099
                     /locus_tag="PKN92_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ47310.1"
                     /translation="MPEKHEHKPLRSTLPGIVLFRLIFSFHLAFLPVFAAGPERWDSF
                     RGPYGNGIVAPPGENTGVPLTWSENENIAWKTEIPYQGWSTPVIGGGRIWLTTATVDG
                     HDFYVLSIDQENGQILLNKHLFHSDNPEELGNEVNSYASPTAALEEGRVYVHFGSYGT
                     ACLDTATYEVLWTRTDLPCRHFRGPGSSVILFEDLLLLTFDGADVQYLAALNKNTGKT
                     VWKTDRSTEWPDIDENGEVLREGDFRKAFTTPFLVREKDRTLLFSQGSYATFAYDART
                     GKELWKKTHSAYSPAPCPVSGNGFVYIPIGRGVQELWAMKLSGTSSIVEKEASWIIAG
                     KKVPAEPSPLLINGLLYLLSNDGIVTCLDGNSGEEYWSERIGGNYMASPIYADGRIYC
                     AGTRSKITVLREGKQFEKLAVNELDEGMLASPVVAGSAIYLRTKTHLYRVELKKEPGE
                     NN"
     gene            complement(51096..52214)
                     /locus_tag="PKN92_00565"
     CDS             complement(51096..52214)
                     /locus_tag="PKN92_00565"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNZ47311.1"
                     /translation="MKTHHIAQKFDVAGRAVTLEHTGEGNVNDTYLAIFRTVFSEERF
                     ILQKINKRVFPHPEQIMQNMRVVTEHVHRRIEEEMDTADRIWQLPRVIPAKDGKDYVL
                     DDDGEFWRAITLIASAHSYGRVQNIEHAHEAGYVLGHFQRMISDIEIDKLHDTLVGFH
                     ITPGYLKKYDEALASPEGQERVKISPEAQRCMRFVEARRDWCSILEDARDAGELKVRP
                     VHGDPKISNIMIDDATGKGTCIIDLDTVKPGLIHYDFGDCLRSCCNPAGEEVRDLGQV
                     VFDTDWCEAIVKGYMVNARSFLTEADIYYLYDSIRLISFELGLRFFADYIAGNVYFNT
                     RYDGQNLNRARVQFKLVESIQAREKQIRSILDKSLRGC"
     gene            52460..53158
                     /locus_tag="PKN92_00570"
     CDS             52460..53158
                     /locus_tag="PKN92_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47312.1"
                     /translation="MFGFRKKSSSSEIILYSYPTFLYVWPVIAFGYVFAGLQALGWAS
                     PTSLAWTYIFIVALTMLTMGVDLGRNTSIFCLVAFLACWLGILWLQEARNVEFFAKLG
                     AMIRSLQPQINPHFLVTLSTLLLILYILMLLLSRVNDRWRITHNEIEHRSFGQKEDAI
                     GRGAKRTRSVYPDILELLICLSGTIEIYTANSNQKLASIENIPFLPLRAKKISRILEI
                     KSVLATEDDDEEEA"
     gene            53346..55226
                     /locus_tag="PKN92_00575"
     CDS             53346..55226
                     /locus_tag="PKN92_00575"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNZ47313.1"
                     /translation="MQFRPFLSKKGGIVACLVWVCLLTHLASAQDRLRQPLEDGVSAL
                     LQYGQTITLEITLSSSSQAQAIISPYLAAPSGWRSFVRGSAALIPFERLSATKQRHVL
                     LTLFPLDYVDAEGWHHHVRIASDKSPETVKILSYWLTGSQDNAALILSVDANRAVKEP
                     LQRGQIILFPQPFLRPVMKEPCASFASRNTDQFAQPFQQQTSPKPKEPPSATYHERLP
                     LMPQAAPPQTPEPTAESAESAMAENYHKDLEYGEDKEGRYAIYRLKPGESLYSAVIVR
                     FTDYTANADVLQACEIVAKRTGIKNMHHVTVEQKVKIPADLLSDQYQPLGTVQRENYE
                     TIREEARRLQEIRKGTRNLQGVILVLDPGHGGIDPGAIRESDGLYEDEINYDIACRIK
                     EILEKATQARVFMTLEDPSQGFKPTNNKRFVHDKDERILTTPRYTIANKDIANNLRWY
                     LANSIYSQARKNGADPQKIVFASIHCDYIYDETVRGTMIYVPGVAHRKNEESHTSGIY
                     AQFEEVRQNPVASSTHSQRVQDEALSRSLSASIIRAMRTHNPPLKVHSIGNPVRSEIR
                     KSGGKSYLPTVLRNTAIPTKVLVEVANMNNPQDRQHLANPEWRQWFAESFVDALISHY
                     NK"
     gene            55272..56258
                     /gene="hemB"
                     /locus_tag="PKN92_00580"
     CDS             55272..56258
                     /gene="hemB"
                     /locus_tag="PKN92_00580"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNZ47314.1"
                     /translation="MILPDRPRRLRLTSTIRRMTRETRISVDQLIAPLFIREEEQVRS
                     PISSMPGQYQLGIADAAEEAQRIYDVGIPAVLLFGIPEHKDNEGSSAWRKDGVIPRAV
                     DRIKKRCPDLCVITDVCLCEYTDHGHCGIIKKTRRNTLDVDNDATVEVLVREALAHAD
                     AGADIIAPSDMMDGRIGAIRSALDAHSFEDVAIMAYSAKYASSFYGPFRDAAESPPAF
                     GDRRSYQMDPANALEAQREISIDIAEGADIIMIKPALPYLDIIRQAADNFDVPVAAYN
                     VSGEYAMVKAAAARGWLDEKQATLEILTGICRAGASLVITYFAQDAAKWLQK"
     gene            56271..57563
                     /gene="hemL"
                     /locus_tag="PKN92_00585"
     CDS             56271..57563
                     /gene="hemL"
                     /locus_tag="PKN92_00585"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016739165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNZ47315.1"
                     /translation="MIHTKSKQLWDEAQQIFPGGVNSPVRSFKSVGGSPFFVHKGQGA
                     LLQDADGNEYVDYVLSWGPLILGHACPEVVQAVCEAAGRGFGFGAPTEVETLLAQKIR
                     HHFPSMQMMRFVSSGTEAAMSAIRLARGYTGRNIIIKMDGCYHGHADSLLAKAGSGIA
                     TLALPDCPGVPEGITENTIVAPFNNAESIAEIFKAHPGEIAAVILEPVAGNMGVIPPA
                     PGYLSALRSLTEKEGALLIFDEVMTGFRVAMEGCQGLFNVTPDLTILGKVIGGGLPVG
                     AYGGRRDIMMHLAPPGPVYQAGTLSGNPVAMAAGLKTLEILGKPGLFGALTETMQKLC
                     KELGALFARHGIPVYQTQAGTMACTFFSDQKVNDYKEAQKSDTALYARWFHAMMQEGV
                     YWAPSQFEAAFISSAHDAVAIERTLDAAEKVLARGLPG"
     gene            57605..60031
                     /locus_tag="PKN92_00590"
     CDS             57605..60031
                     /locus_tag="PKN92_00590"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47316.1"
                     /translation="MLTFSALLLMTTLHLAQVQPAVSFETALPVWIEGREEEMNLCAG
                     FRCAFNFPGSEPVHAKVTASTLYRLYVNGAFAGYGPARGPRGFFRVDEWDISSHLRAG
                     TNVVAVEVAGYNANSYYVLDQPSFLQAEIGTKNKVLAATGAGPNQFKAHIITERLQKV
                     QRYSFQRPFIEVYALKPGFDLWHTRVDQSLPRALLSVCEPKTLLPRGVPYPLFPIQPA
                     VKIISEGTMRSGENDNPFLDRSLMQIGPKLKGFVREDLAVEISDELCRLHPDHCEAVA
                     EENAKPGNFTLKPNEFKVLDLGCNLSGFVFLKVNCTEATQLYITFDEQLDGLDVNWRR
                     LGCVNALTWNLAPGTYTLEAFEAHTLRYLKVFAAKGNVTISHAGMRLYENPEAGKAQF
                     TCSDERLNRIFEAGRTTFAQNAVDIFMDCPHRERAGWLCDSYFTARSAEVLCGNYSVE
                     RCFLENFLLAESFPCLPDGMLPMCYPADHYDEVFIPNWAMWFVMELGEFADRINDPAL
                     IARFKPRVEALFNYFEKFENADGLLEKLESWVFVEWSAANKFVQDVNYPSNMLYSGAL
                     ETAARLFDVPAWAEKSDKIKERIREQAGKELFFVDNAVRDADGALVITDNRSEVCQYF
                     AFYFNIATPEKNPELWQILHSEFGADRIEKGLYPEIHQANAFIGNMLRFELLSRAGLA
                     DQLVKETADYLMYMVERTGSLWENIQDAASLNHGFASHITVTLFRDILGVWKITPKNK
                     TVTVKINPVSLDYCSGIIPVGDDFVEASWKKDGDSIQLRLSLPQGYTAEVENNSGLTL
                     RMDNPATKES"
     gene            60224..61630
                     /locus_tag="PKN92_00595"
     CDS             60224..61630
                     /locus_tag="PKN92_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ47317.1"
                     /translation="MAQIAHASKRSRHNIHKHRLLIACPDITFGTTLVNFLKAFPIEA
                     EQCHDRAGLLRSVSRRQADIVLLDLSLNTDETLNLVSFIRHQAPSARIVLLFEMDQLQ
                     QALDGIRHGAYFYLPKSCPLSDIALIIGKALENLSTEIHLDSYEQTVFEEFIGRTDAL
                     RRIIELIKKVAPTNSTVLLLGESGTGKEVVAQTIHRLSTRREMPFIAVNCAALPDTLL
                     ESELFGHVKGAFTGAHQDKQGLFEAADGGTLFLDEIGELPAITQAKLLRALQGGEIRR
                     VGAIESRKMDVRIIAATNRDLLEAVEDFRFREDLYYRLNVIQIRIPPLRERLDALPAL
                     VTHFIAKCNQSYNKSVRGVDQAAAAYLQQYPYPGNVRELESIIAHAVIVTESDFIGAA
                     DLPEYVRQGISHRPALPYHKEDAILSLTEMEANHIRFVLDATNWNQTHAANKLGISRS
                     TLWRKMREYNLQAPPDSV"
     gene            61729..62085
                     /locus_tag="PKN92_00600"
     CDS             61729..62085
                     /locus_tag="PKN92_00600"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNZ47318.1"
                     /translation="MSEQQHGDRRRHPRCSKRVAFCWCESEHGTSNHVDNISESGILC
                     QTGAPIPLMTKIQVLLDLPKPFEQRIEVDGVVVRCEADPFKDNCFKVAIFFSGIKDAD
                     RDTIRRFVASSEKTEE"
     gene            62133..63494
                     /locus_tag="PKN92_00605"
     CDS             62133..63494
                     /locus_tag="PKN92_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47319.1"
                     /translation="MDKNTLTRRAFLASATAGSVILSGLPVRANTARVVPRKISPNEK
                     INVAGIGVGGKGMGDIMSCRRENVVALCDPDKKRAAEAFSRLSKAKQYDDYRKMFDEI
                     GDEIDACTISTPDHSHAPAAYLAMNLGKHVYVQKPLTHTIAEAALLTRLAAEKDLVTQ
                     MGNQGHCEDGVRDLCEMLWAGAIGDVTEVHIWTNRPIWDQGMENPLPEEPIPAEMNWD
                     LWIGTAPMRPYNKGYAPFNWRGWWDFGCGAIGDMACHIMDPAFWALKLYEAEDFTVQT
                     VRQEGMTKQSPPRTSVIKYQFPQRGAMGPVAVYWYDGGEKPTYPESLPPGQKLGDGDN
                     GSLFIGTDGFLTAGEYGGRSRLLPDTRMQDYKRPDPSIPRIPKGNPYQNWLDSIREGK
                     KSASDFSYAGPLTITANMGNVALQAAGEALHFDARALRITNNEQANALLTKEYRKGWE
                     LPC"
     gene            63791..64771
                     /locus_tag="PKN92_00610"
     CDS             63791..64771
                     /locus_tag="PKN92_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNZ47320.1"
                     /translation="MSIKLATGLWVYSSMQDRFCLDGYRETPRLPDVISALAKLKGLK
                     GVEIQQTDITPEMPVKEVKRLLKDNGLVCSAVSTSLSHSRRFSLAGFAQQHQKTRNAA
                     IDEGRKAVEIARALGTTEVVLRLFTDGFDYPFHVDYTAHWNTIISSIKTIAKFASPDV
                     NISVVYKPREPRKYLAISSVGKALSLCQEIAMKNVGVALNFSHALMAGENPAESIAFL
                     NRSSKLFQVYISDCYAPWDDLMIPGSLHLWETLEALFYLKNAKHKGFVTIDIQPQRMD
                     PNHALQLALGNIAILWKKLERLDATELRKAQRTLNAVESQRIIRRILLQG"
     gene            64913..66439
                     /gene="hflX"
                     /locus_tag="PKN92_00615"
     CDS             64913..66439
                     /gene="hflX"
                     /locus_tag="PKN92_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNZ47321.1"
                     /translation="MISPEIARTLTELSFDIRRQIGLIIDRRGRITAVVVGDARRLVL
                     PDIHRRGMNRLSGVRLIHTHLTNEALSNDDLTDLVTLRLDLIAALIVQTDGMPGSLYS
                     AHLLPDNAAQDPWNLLPPSSIHALEEDFLDFIEALEEEFSRTQYQLSTDDPRERAILI
                     HVSTLPPSIAHDSLDELKELARSAGIKTVQEVLQRRSIDSRLVLGKGKMQSVLISAMQ
                     KGAELLVFDTNLSPSQLRSLGDFTDLKILDRTQLILDIFAQHAVTREGKLQVELAQLR
                     YLLPLLNIRQKALSRLTGGIGGRGPGETRLEIDRRRARDRIARLEREVAQLGERRRLR
                     RQVRTEKNVPTVAVVGYTNAGKSTLLNHLTNSDVTAKDFLFATLNPVSRRLRFPQERE
                     IIITDTVGFIRELPEDLMAAFRTTFEELDEADLLLHVVDAHSPDLSDKMKTVLDTVRS
                     LELDHKPIVTVFNKIDLCSPEEIEGLLHQYEGVPVCALDRGSFAPLLTLLEERLWPAD
                     "
     gene            66448..67386
                     /locus_tag="PKN92_00620"
     CDS             66448..67386
                     /locus_tag="PKN92_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47322.1"
                     /translation="MLRIVVALVLSVLFQTASHAQPWQNPPEAPPPSRQDMINRFQQL
                     TAAGAPFNVSAELYEEYLGLIQNLDYQDLWADLLEKKLNEPGNQEALDVKNWLALGEA
                     HFKTGMHGTLKAAEAFQHALTLSPGHADALARLGQLHHREGQYDLAESKYRKALESDP
                     ENALALIGTAVLQIRKGDILNTSRTLDQLGVKAQPYDVETRLMLRQALTAFEHHGGWF
                     DDTAENHNAYARLLYRSGRLTDAILAARRAVTLEPARSDIWNFIASMQLQIGNLEQAK
                     QAYDQSLEANPDQPAITDTRRQLITTLTTSGPGKAP"
     gene            67436..68401
                     /locus_tag="PKN92_00625"
     CDS             67436..68401
                     /locus_tag="PKN92_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ47323.1"
                     /translation="MLRKKRLVLYLLVAGLIWIGWRYSLPLAGVLLLTAGVLLGLVKL
                     LRIAQAHFGMKRLNEKTNQRYAALIQKLPGQEESETWSFAVLGDTRNNVQIASALYKK
                     IESYAPVMAFHTGDIVRGGTADELLDRHIAVVERDMPSIPLFCIPGNHERGPKRDYAA
                     FKRLYGDDRFSFSFRKCLFVGFNNNLKDYVQEEDLSFLQEQLQGEYRHRFVFLHIPPA
                     FFEESFVSDTRRRGFKKNAEVFHQMMVEHRVDEVFMAHIHGYATLDRDGVRYTLTAGG
                     GAPLSHRIAPENRHYHFIDCQVTPSGITRTLHLYIDGRWQEREIR"
     gene            complement(68414..69556)
                     /locus_tag="PKN92_00630"
     CDS             complement(68414..69556)
                     /locus_tag="PKN92_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNZ47324.1"
                     /translation="MYKTGGILDRLLNSFIFSRRRFCTAESGTVLNAAVYETATLPVS
                     SQDDPEDCAGQGFLSSLSSRSSSAAKVCVIVNPHSSTGKTLKRWPSIFKTLEGHIGKI
                     DYVFTKAPLDASYLARAALKNGAEHIIAVGGDGTLNEVVNGFFENDLPVNPSAVLSHI
                     CSGTGCDFNRSFSSSRDFYKKINSIAEGKTRRIDVGRVHYHNDRTGETEIRYFINSTS
                     CGLSGATCRTVNSLVFWKKLGGKIAFQWGVISTLLRYKSKKLRLRVDDHYDEVLEANT
                     IAVCNGRFFGGGMQIAPEALLDDGVFDIIAIRKLSLFQVLKWFPLIYKGTHLKKKDMV
                     VALRGKKVSIEPCQPEDAALFEIDGESPGGVPAILEILPAALLIKQ"
     gene            69773..70471
                     /locus_tag="PKN92_00635"
     CDS             69773..70471
                     /locus_tag="PKN92_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ47325.1"
                     /translation="MKHCMSVLVLLAFFCLYMPSYAEESSCSLHPDSCAWKPLFADDL
                     SNTVKPDGVWTVENGELTASKDEAIWTDREYENFILDLEFKNAEETNSGVIIYCSDLK
                     NWIPNSIEIQIADDFAEKWAQSPKSWQCGAIFGHLPASKSVVKKPGEWNRMTLRCAGK
                     IIEVILNNEKINEINLDDYTSAKVNPDGTEIPAWLSKPKSELATKGRIGFQGKHGNAP
                     VWFRNIRIFETASN"
     gene            complement(71041..73080)
                     /locus_tag="PKN92_00640"
     CDS             complement(71041..73080)
                     /locus_tag="PKN92_00640"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNZ47326.1"
                     /translation="MKMKSFLPFLVILVLLLGLVVWQKSNQKKPAPIAEQIGLAALVP
                     DTLTKSTLDRVELFSGSAPEDKVVLQKKREEWLITSLHNAPADKNAVDDFIGKILAMK
                     GEPRATADNDEKLANFALKDDEAFHVQAWLASGESPALELLFGKAADFRTVFVRKAGE
                     SKVFVENNNLRREAGASETGEDLRPKSNKWLKTQLFDVDAENVAKLTLKYPDKELVFD
                     RKELEKEPETQEAGSEESAVPAQPDKQYEWALATDPGAKDFKSTEVKTILTKFSTITA
                     MDVAGPDEKDTCGFNPPAFTATVSFADGSEKVFYGGRNVAEDKYYLQQAGQEPELMYQ
                     VAKYNFEQVFVAGSKIVSLPEWNLEQESLAKITMERPEGKVVLEKDGSDWKIIEPQLN
                     LEQQTTALKGIATALASLKAIDYADQAADTGALDTTIRIAMLDGSERVLRAGSPSLSV
                     DGRYVKFDDSDSILVISRADSERILPPVRDLFSLAVLDIPSENLVELNVTQEKRNLAL
                     KKDPQTEQWAGTFNGKPIAPGADKVEDYLMTVNAFQMDSFTLSRAGSEVEAAATLSFV
                     LADGEHKVLSFGAEKDGNYEASLSWLPCLFTAKSLEYLRLFADIDAFAAAEETSGAAA
                     ASSATETAGAGSVVEENASGMIPKEESVVAIDLPSASSGQEAPEAIGDTSEAPSE"
     gene            complement(73114..75642)
                     /locus_tag="PKN92_00645"
     CDS             complement(73114..75642)
                     /locus_tag="PKN92_00645"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNZ47327.1"
                     /translation="MSNTLSILRRDLGTYFTSPIGYIFIMVFVTMSVGLYVTAFFLFP
                     VADMRPYFENLPLLLCVFIPAVTMRIWAEERKENTWEMLLTFPMQAWELTVGKFLAAL
                     TFYALTLIATFTVPLMLFSLGNPDTGVVVSGYFGTLLLGATFLALGILFSGFFKDQIV
                     AFVVTLLACFLFFLLGTDFIASYIDSRISGLGSMLADLFGIFTHYQPLVRGVIDLSDV
                     LFFLTWTVIFLTLNILFIDGRNRPRARTMFGMITAVCLIIGMLFNYLMAGSSIKRFDM
                     TEDKIYTISPSSKAILQEASSEIEIKLYITPRDKMPTAMRNMERDITDKLEELRLASG
                     GKLNYATVYLEAANAITTGSDFNVGDDKDKTEDEAIEKRMLDKGVEPFNVQAMSEDQV
                     TSQLVYSSIGVGYEAKAEEIIPQIMPNTLPDLEYQLANTIYKLTREEKPVVALVAPKE
                     AVNIDPQMRQMLMQMGQPVPETEDPYVYLERILEMEKYDVQRIELTKEDPLPEKYDTL
                     VVVNPRSLNDRQRWEISRAVYSGKSVIIAAQQYEWDYQATRDGNRITRRDENPEVNQL
                     LEKYGLGVSKDVLMDTNNVTLTVQSQGGGLFGALMGQPFTLPTHILINNKSMNQAKSI
                     TNRLAAILYLWGTAVSMDEAKLKELKLDAEVLMSTSANAWSVAADAPMSQVSFEPPSS
                     GKAYPLMVLVQGQFPDAYADSERPAWPKPQPHPGMPPVPEDDAQEAPAAAVTPAPGKL
                     IVLGCSEMFKKNFLQASNLDLFLNSVDAVTLSENLVNVRGTKPIDRLIDRPTGRQRTI
                     WQLANYGLANVIIAAVGISSFVLRRRSRNAYTMAQSRTAMSNNQ"
     gene            complement(75655..76608)
                     /locus_tag="PKN92_00650"
     CDS             complement(75655..76608)
                     /locus_tag="PKN92_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ47328.1"
                     /translation="MIKAKNLTMHYGPVVALDNVSFEVNRGEIVGLLGPNGAGKSTTM
                     KILTTYLYPTSGTAEVNGVDVLENPLEVRKSIGYLPEILPLYMEMEVRSYLDFVARAR
                     GLKGAALKNRIDAVVDTCGLRPMYRKVIRELSKGYKQRTALAQALVHNPDIIILDEPT
                     SGLDPHQILEIRNLIRQLAEEKTVILSTHILQEVEATADRIVIINRGTIVGDGTLAEL
                     RGRAKQSERLVISLEGEREQFERLLGGIDGVKQVVHRGSSGGCSSFVLHSPVDKNPWR
                     EINALVQQQGWKLRELSDKPLSLEETFLTLTEKAAGQKTAS"
     gene            complement(76862..77824)
                     /locus_tag="PKN92_00655"
     CDS             complement(76862..77824)
                     /locus_tag="PKN92_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNZ47329.1"
                     /translation="MVILKSVDMRYVAMGVSLVVAVLMLLGKMAAYWLTGSYAILSDA
                     AESVVHLLATGFAAFSLWYSTQLPDRLHPYGHGKIAYFSAGFEGALIGAAALSVFYLA
                     GHSLVYGVVLGPLGAGLLITFVLAVINALLGSFLVFVGKRYHSIVLVANGKNVLSDMW
                     TSFGVVLGVGLVWLTGWKWLDPLVALIMACNISITAYGLLKKASQGLLDRACPENTVL
                     LQKCLDEQIQSGCIKSYHQLRHRQAENKLWIEFHALVDRSLSVEEAHRRITVAESAMR
                     SLFPDFQVYITSHMEPGDHKGAHPLEYDEGVDALVADEDRCNLK"
     gene            complement(77861..78721)
                     /locus_tag="PKN92_00660"
     CDS             complement(77861..78721)
                     /locus_tag="PKN92_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNZ47330.1"
                     /translation="MTSKKRFLIPDGYSKESRDEFDRVGMTLAGKLYASLLMKRMPDA
                     EYDIWYSSDPGVEAPTDEELEGYAGVIWPGCNLTIYHDDPRVKAHIKLCERAYEAGIP
                     QFGSCWAIQLATTVAGGVTEPHPRGREMGIALNIRQTEAGMRHPMMKGKPPVYSHFVS
                     HDDYVARVPECAEILAGNYWCDVQAVAVTYKRGVFWATQYHPEYDLKEMACLIHAREE
                     RLIKQGWFSDREDLIAYADKLEKVFQEPDNTKNIRWQLKIEDDVVLDDIRECEFVNWL
                     KYAVLRESLV"
     gene            complement(78811..79773)
                     /locus_tag="PKN92_00665"
     CDS             complement(78811..79773)
                     /locus_tag="PKN92_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ47331.1"
                     /translation="MAVFELSLDQLKSYQGRNPKPTDFDDYWDKALMEMRSIDPAVEL
                     VPADFQTPFAECFHLFFTGVQGARIHAKYIRPRHASQPHPALLLFHGYTGSSGDWADK
                     MGYAALGFSVAALDARGQGGRSQDTGSVLGNTHCGQIIRGLDDHPEKLYFRQVFLDTA
                     QLAAIVMHMDEVDSTRVGAMGMSQGGALTLACAALEPKIARLAPMCPFLCDYKRVWEM
                     DLAVDAYAELKTYFRLFDPRHERENEIFTRLGYIDCQHLANRIKGKTMMAIGLMDEVC
                     PPSTQFAAFNKITAPKECVLYPDFMHESYPGFRDKAFQFFSEMI"
     gene            complement(79818..80579)
                     /locus_tag="PKN92_00670"
     CDS             complement(79818..80579)
                     /locus_tag="PKN92_00670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ47332.1"
                     /translation="MTKKAIGAVTFDLWDCMFCDETDEPKRAFMGLPPKKVARRQLLH
                     EALATEAPIDRLLVDHAFDTADAAFRKVWHEQYVTWTTKERIEVVLAGLGRTLTEQSL
                     TRVVDGIERMEVDISPDMAPGVRESLAVLKEKYNLAVVSDAIFSPGKYLRELLAKHDM
                     LKYFDYFVFSDEIGHSKPHPSLFEAVASHFGIQVDEIVHIGDRPHNDIGGPHAVGARG
                     LLLTVVKDRPLDGHVPDAICNNYAELADVLASMEP"
BASE COUNT        20661 a        20718 c        19152 g        20168 t
ORIGIN      
        1 gtctccgtcc gtgatctgcg tcaatttgcc gtgcagaggg gcttcactgg aagtaacagg
       61 tttatctttg gaaacaaggg cagtgccttt cggagccatg aaaggcgggc gatctttgaa
      121 atcttcaggc tcaagattgg gagaccagta gtcaataggc gttccaccga aaaaaggctc
      181 aggaagttca attttaattt ccacatcatc agcaacagcg acaggggccg cagcaagtcc
      241 gaggcatacg aacccggcga gcagggctgc cgaccacaac atactcttct tgctcatact
      301 acactccttt taaaaaagtt ttgggggttg aaaaggtggt tacttagacc agtcagacac
      361 cgtccgcatg cctaacaggg ttcagtatac catacaagag acacttaact cacttcttta
      421 tgcattttgt acggggaaga gtgcattttt tcactccttt ttcctgtaac tgatgcacgc
      481 gctgcatcaa tgcgtttatt agaccgaagg gatccagttt cagtttctca ggcgaccctc
      541 aatcgttgaa gggcagccac gcctttcatc agatcagacg attacgggag ggatctattc
      601 attcttaaat agatgtctcc ggatcttctt ccgccataac ctctgcccgt tctctaatgc
      661 tttgcagctt tgcctccttc ggtttcaaac gctcaataat ggtgtcctgt tctcttcgga
      721 tttccatttc tctggagaga agggattcac attcctgttg aacggtttct ataacgtgct
      781 ccagttcctt gcgcatagcc tgttcaaaag cctcaccgcg ccgctgtgaa taattaagaa
      841 gcatcgtaag cacggactct gaggcagact cttcctgtgt ttcctgcatc agaagtttat
      901 gagtttcctg tcggagtaca ggcataatct ttttgatcca tcgctcttcg ctccaggcaa
      961 agcgtgcaac ccaccctctg atagtaccca tgaaaccgcc cacgccaaca cctatggctg
     1021 caccgacagc aggaataact gaggaaatgg attttcccgt aaaagtattc aagcctgcac
     1081 cagcagaacc gaaaaccttt ttctcttccg actgcgccgc atcccaggcg acattcacta
     1141 aacgctcagg ctcgctcaat gcggcacgca attcacgggc gcggctaatt tggcgggaca
     1201 cttcttcgga agtacgtttt tcattcgttt cgatgacagc acgaagtttt tttatgacgc
     1261 ctgtgatgaa aacgtccact tcatgctcca actgcgccgc cagcggatga aaattcccct
     1321 ggcgaatttc cttgagctgt ccttttttac ggagccagtc caataaaggc tggataattt
     1381 cagattctat caaaggtact gtcagctgcg cccgcaggtg cgcatcatag cccggaccga
     1441 agacttcatc ctgttgccca ccgtgaaact ggatattgta acgcaccaga attgaatcca
     1501 cttcggtacg aatggtcgcc atctggttgt tcaataaatc ccggttttct ttcagttcaa
     1561 tgaatttttc agggttgcgt gccagagcaa gagtattgct tatagtgatc agatagtctg
     1621 aagcacgttc ataaacaaag cgtgccgctc tggtaaagac ggcgttggat ttattttcat
     1681 tgatcagata atccataagc cgctctttca acagaggaaa gcggctttgc cccatcagaa
     1741 aagcagacag atcctcttcc ggcgtatcgc tgtgctccag gcgttccaat atggaagtgg
     1801 gcagcatcag tttgttgtca gctaatgcct cctcaaagga gagacgtttc tgatccagtt
     1861 ccagtgcact caatgctctg tatccggaca ggaagaagat gtcagaatga tccggaatat
     1921 cattgcggcg aaatgtctcc accaactgcc cggcaggccc gaaagtgcct ctgggatcaa
     1981 tttcgtcgtt ctccagttta tccgctttat tcagtacaaa gaagacccgg cggtcacggc
     2041 aggtaataat tcttttgatg aaattcagat catggatatt tccgacaaaa gcactgtcca
     2101 caaaagcaat gaccagatgg caatgctcga taatgtcata ggtaattttc tgccgtgttt
     2161 cggaaatgga gtgtacgccg ggcgtgtcta tcagtacgag atcatcctga agtgcctctt
     2221 cggggaggca taatatgagc gattccgttt tttcccgaag agaagagaga tgcgggaagg
     2281 cttcgtccgc gagaacagta atcagaggtt cgagagcacg ccgcatggaa tccggattct
     2341 gatcctggca caacacagaa atctgaagcg gatttccttc actttccacg atgacaggca
     2401 cagctgcaag cgctcgctcc agtgcttcta tttcgtggcg ggatgccgcc gtatcaagag
     2461 agagcagcaa ctgcattctg tctccccgct gaataaaagt caggcgcgac gtgcactctt
     2521 caatatccat gggaagataa gagccgccca ggaaggcatt cagggctgtt gatttaccca
     2581 cattgaaagc tccgagaaaa acaacccggt atcttccgtc gcgaacaacg gcgtgctggt
     2641 aggaaacagc tttgctttcg taggcacgat cgccgcagta atcttcatcc agacgtgaaa
     2701 aattctccag atattccaat gtttccagaa gccacatccg attctgttcg tgagaattgg
     2761 tcatcatgcc tcctgctcct gctggttgtt gctgataagg gcggtaagta aaggatgaac
     2821 aatgcggtca agggggcagg taccctccgg agtctggcat ttcgcttcaa attcatacag
     2881 agctacacgc aatgcgtcaa aacctgcatc gcaaatatca cgtacttctc ttttgacccg
     2941 gtggcgatat tcgtcatcct gattgcggcg cgcaaactca agttttgccg acacaatata
     3001 aaagggcggg gcttttacat ggctatggct ttctatgcgt tgcgccgctt tgtctctgat
     3061 tcggagtata tctccatgat ttaattgatc tgccatattc gctaccacaa gaaagcgtcc
     3121 ggaaaaccgc tccgcatagt ctgttatcaa cgccagtgta tgacgggcag gccagtactc
     3181 cacagtagtc acaagaacaa tcagggactt gtcagccgcc tttcctgccg gtagcagcgt
     3241 atcttccgca acctcagttg aggtgcccgg cgtgtcaatc agcgttaacc gtgtaaatgg
     3301 aaactgaggt accagaacgg acagcgcaga aaaggggcat tttgttctgc catgacgccg
     3361 ggtgctgaga aattgtgtat gcgactgctc agtaaaagtt ttttcctcgg gattgtaggc
     3421 actgtacgca atcgtttctt ctcctgaacg agtcaggaag acaggggggg tccggctttc
     3481 ttcccggctt gtaaaaagaa gatttcgtcg catcagggca ttcagcaccg aagatttccc
     3541 cacattgaaa tcgccaacaa aaagtgccgc cgcttccggt gtcgcttgcg ccgtttggga
     3601 aaaagagttg ttcatagctg atttttcttc ctcatcatgc atagttgtga caccctgacc
     3661 aaatctggtt tcagtgtcca tactcagcag tccttccggt tggtaaatgc caccagggca
     3721 ttgagttttt ccagagcagc accgctatca atggaatagc gtgcccgttc aaccccttct
     3781 ttcaatgtat ccacaagccc gcccgctacc aatgccgggg cagcattcag caataccacg
     3841 ttgcggcagg gcccttccat tccttccaga acgtttcgcg taatttcagc gttgacctgt
     3901 gcatcgccgc ccagaatctc tgccattgcc gcaagaggaa tacccacttc ttcagggttc
     3961 accagatatt cacggatatc attatttttg gcttcacaaa cgtaactggg gccactgatc
     4021 gttaattcat ccagcccgtc gcgacccgca acgacaaagg catggcgtgt tcccaaagag
     4081 agaagcgccc gtgccatcgt cggcgcaagc tggctgttga agaccccgac aacctgtcgg
     4141 tcagcattgg cgggattggt taatggcccc agaatattaa agatggtacg gatttttaac
     4201 tcgccacgga cgggtgccac atgccgcgcc gccagatgaa gagatttggc aaaaaggaac
     4261 ccgatcccca gtttatcgat gcttttgcct acctgatccg cttccagctc aatattaact
     4321 ccgagcgctt ccagcacatc ggcactgccg cagcgacttg atgccgcacg gtttccatgc
     4381 ttggcaacgg cgactccggc accggcaacc accagtgccg ctgtggtgga aatattaaag
     4441 gtgcctatgc catctccgcc ggtgccacag gtgtcgacca ggggcgaacg ggaggtctgc
     4501 acaggagtag caaattgacg catcacctgg gcaaaaccgg taatttcttc tgcagtttcg
     4561 cctttcatgc gcaacccgat cagaagtgcc gccgcctgcg ctggcgttgt ttcaccggac
     4621 atcagtttat ccatcgcctc tgcgctttgc tcacgggtca gatgtgcgcc gcagcataaa
     4681 agcgaaatta ggtcatgtat actatacatg ggcatgagca gaccgactcc ttattttctt
     4741 tatagcaacc ccaaccctga ctgaagccta taatactgtt tttatgagac catgtcaaag
     4801 cctgtcacct cacaaagtct ttgccttttc cctgtcactt gtcggttttt attcccctgt
     4861 ttcaccgaca aatatgaaaa gtttttcagt aatccctata tattggggaa aaatgagttt
     4921 tcaagcactt ttcagcctgt ggataactta tccccgtacc tgcatgtttc aggagtcatc
     4981 gtttgaaaaa gaaaacagtc aataaacctt agtcaccact acactccata tcttattgat
     5041 acacaaatgt ttaatactta ttttggggga gcgcttccag tgtttatttt tattcctcaa
     5101 tagaatcatg cgcttttctg ctgtggaaaa cctgtggata acttgtgttt ttatgcttta
     5161 caacatatag tcaacctgaa ttaattttta aaaaatgacc ctgtaacagc gtctttgttc
     5221 gatttagcaa cgtttttttg atatgctttt gtttatagta attatgtcag ctaaaaaagt
     5281 aacaagaaga gaacagcgag gagatagtat gagcagcatt tcacgcagaa actttttgtg
     5341 gtcttctctt gcgaccgccg ggcttgccgg gctccatctg tcccggacgg ccgccgaaac
     5401 taccattcag ggctttgatg aaacggggac aactcttcag gcaggtgcca tatggcagcc
     5461 tgtttccgac cggaaaatca aggtgggtat tgtaggatat ggcgtatgtc ggttcggtgc
     5521 cgcttttggc tttcaggatc atcccaatgt ggaaatcgtt gctgtcagcg acctgcttcc
     5581 ggatcgctgc gccgccctgg cgaaggcttg ccgctgtgag aaaagctatc cttctttgga
     5641 agacctgatt aaagacgacg aaatcgaagc agtctttatt gccaccgatg cgcctgctca
     5701 tgcgtggcac agtattactg ccatgcgcca tggcaaacat gtggcgtgtg ctgtccctgc
     5761 tgtttttggt tctctggaag atgccgacca gctttatgag accgtaaaat caacaggcat
     5821 gacctatatg atgtttgaaa ccacggcttt ccgcaacgat gcctacgcca tgcgtacatt
     5881 gtacaatgcg ggagcattcg gcaacatgct gtatacggaa ggcgagtatt accactacat
     5941 ggatacgccc atcgattctt tccgtgggtg gcgcatcggt ctgccgccgc aatggtaccc
     6001 gacccattca aacggatacc atacctgcat taccggtggc ggctttacag aagtttcgtg
     6061 cctgggcatg cgcagtcgga ttgattgttt cgatccgaag aacaaccggt accagaatcc
     6121 cttcgctaca gaaattgcac ttctcagaac aagcgaaggc ggttctgctc gcatggctgt
     6181 cagttgggat acgccgggac tcgaagggga agtggggcgg gtacgcgggg aaaaggggag
     6241 tatgaccgac ctgaactatc agggactcgc taacatctcg tctgtctctc ttgaaaaaac
     6301 gccgcttccg cctggaatgg aagcaggcgg gcatggcggc tcacacggtt atctctgcaa
     6361 tgagtttatt atggctcttc tggagaggcg gacacccctc gtacatattg cggaagcgct
     6421 gaatatgact gtggcgggca ttgtagccca tataagtgca ctcaaagatg gcgagctgct
     6481 taaaataccg caatacacac tctgatcgca gccaagactg aaacaaaagg acagcctccg
     6541 gagatcttta aatatcccgg aggctgtccg atttttttta tgtcggtcag ctaaaggatc
     6601 agagcgagag gctctttttc agaaaatcaa aactctcctg cgcacactca aaaatatctt
     6661 tttctgcatt gtcctgctcg taaatatacc actcaacgcc ggcttctttc cctgcttcaa
     6721 aaatcgcctg ccaattcagc gcaccttgcc ccaaaggcac aaactgagcg cgccgcccga
     6781 gttttctggt ttccagacag tctttggcgt gaatcatagg acatctgttt ttgaactttt
     6841 ttatatagtc cgccggatcg gcaccgccga gaaaaaccca ggcaagatcg agctcagcgc
     6901 ataaatcact tgtcgcttcc ataaggatgt ctattttggc gcgcgggtca ttatcaaatt
     6961 tttcaaattc ccaatcatgg ttgtggtagg aaagccgcat tcccgtttct ttcaaccggg
     7021 cggcgactgc gtccagccgt gccgccccgc gcagccaggc ttccagatct ttacggcaat
     7081 cagacatcat accgccgggc gccaaatcag agttgcccac tgtcctccag aaggcaacct
     7141 gaccgtcaaa atcagtctca aaagcctcga tcccgatgtg gcaggaaacc gctgtcaatc
     7201 cggcattatc cagtgccgca cgaatttctg acgccggaag atcaggcatg ccgctccact
     7261 gtatgtattc ccagcccata tcccgaacct ttttaagagt gcccggaaga tcttttttgg
     7321 ccggttcacg cagcgtgtac atctgcaaag cgatcccctt gaatgcgctt acctcatgtg
     7381 caaaagtacc cgacattttt gcagatgccg ccagtgcgcc aaaaacagca agcgcctcgc
     7441 gacgggtcat cttccacccg gattcatgtt tcatgatgac tgtcctttct aaacgattga
     7501 gtgcctgatc agtttcatct tttttacctg ttcattatac aacacatcct gccatttaat
     7561 cctgccgggc ttcagtcaaa ttgccgggca gcaaagggat gctgtatact gcgtactaat
     7621 acaccagacg gtaacgcagg aattttcttc aatgactctc aggtaagtcg gagccagcta
     7681 tgattcagga atttcacgga acaactattt taagcgtacg aaaaaacggg cttgtagcca
     7741 tgggcggaga cggacaggtc accatcggcg acactgtcat gaaaggcaat gctgttaaag
     7801 tacgccgtct ggcagagggc gaagtgctcg ccgggtttgc cggtgccgtt gcagatgcct
     7861 tcgctctgtt cgagcgtttt gaaggaaaac tgaaggaata caacaaaaac ctcctccgtt
     7921 ccgccgtaga acttggaaaa gagtggcgca cagataagta cctgcgacag ctgaatgctc
     7981 ttttagccgt cagcaatgcg gacatatcac tgctgatttc gggcggcggc gaaattattg
     8041 aacccgaaga cggcattctg gctattggtt ccggaggctc ttatgccctg gcagctgcgc
     8101 gggcattgtc atcgcacaca gatatggatg cccgggaaat cgtggagcgt tcgctggaaa
     8161 tcgccgccga catctgtatt tacaccaatc gccatatcac cattgagtta ctctagataa
     8221 tcgtcatgcc catccggctg ttgccagact ttttcagcag gatcaccctt tttcaaaagc
     8281 cacacttcgg caattctgga ctgttgtcgc cagtttatat gctcttcttc aatggcagtc
     8341 cagtcaataa tcagggtacc gccggttgta atggcattgg gtatgggaaa ttcttttact
     8401 ccgcccaatt ctgtgctgta ggcagaaggc accagcgcaa aaccgttcgc acggggaaat
     8461 gcttcaccgg caccgataat gcggagtaca tattccgcat ccctttcaag accttcgtac
     8521 atcatggaaa aggggatgct taaggtgctc tggtgggtca ggcgctgcct gctgaatccc
     8581 tgatcccacc accaagcctc cggattgccg cagcgctcca tttcaggatc ggtattcatt
     8641 gcctcagccc gtttcacatg aggtgacttt cccacctgtc ccacagcgtc atagaaagaa
     8701 cccgatccgg gactttccca aagcgcaatt tccgccagcc tgtccagttt ttcctcttct
     8761 gaagacaggg ctttaatttc cgcaaattga tcctcgagcc accatctgtt gcttaaagga
     8821 taatcgataa aatccagact gcagccccgc tcatatcctc tggatttata tttgggcacg
     8881 cttgactgga gaccgattga atggaagagc tgatcataga gggcaactac tttttctctg
     8941 agtactgaaa tgccagcggg cacttcggca gcattcacaa tattcaatgc ctgatccatg
     9001 gctgcatccg ctcctgattt cgacgctttg agcaggacag cattagcctc tttttccagt
     9061 gcacgctcat gaagaagccg ttcacggacc agcgcatcat aataagcacg aacaagcagg
     9121 gactgccacc gccagtcgtc ctgcagtcgg ggatatcttt tttcaagatc ctgccaaagg
     9181 gtcaatgtcg ctgcaacacc gccgttgctt tccagatctc ccgcccaatt ccgttccagt
     9241 gcggctatgc catccgcaat cagggtttct tctccaaccg ggaagaagaa agatgcgtat
     9301 tcagtaagga tatcctgaac tttcgcttca ggatcccatc cgcgacgaga ccacacaatt
     9361 ttattcacat catcattaat tccgtcggaa taagtaataa aaccaacggt cataggcgca
     9421 tagtagttgt gcacaaatcc gtaaaaatgg ggcatcgggt tgatggcttc ccggcctaaa
     9481 gtcagcgcaa atgcatgatc ccaccaggga atggggtact ggcagcggac agtatgtgtg
     9541 atgtcgggat aatgccttac cggatacgct ttattcaggc gtttgcgtgt ttgcggcaca
     9601 tcagggcttc ccggtcctga tacaagaccg ccaaaccatt tgggctgttc cctgtctatc
     9661 cagcgttgta tatcttcgca ctgttccgct gtaaaccatt ggagggagag ccagattttc
     9721 gcttcaggat gcgtgcggga aagcggcttt accagatcct caagaaaagg aagcaccagc
     9781 tcagggggat tggcgccggg atcacctccg ggaaagaaaa ccccggttaa ttccgggcag
     9841 tcattgaaaa gttccttata ctctgcaaga tgagccttcc gtttctccag gtctgccaga
     9901 tcaaaaacgg caggtgtcca aatccagtaa tcaagcccgt aatcagcaca aatttcactc
     9961 attcgccgat tcattgtacg gcggtcaagg ggcatatgag ggcttggaat ctcatcctga
    10021 aaaggaatat tttcgatgct gttggttccg aataaagcca gttcacgaat atactgctca
    10081 tattgcctgt cgtcccaact gtcataactg ttggcattgt accggtatcc cagctgatgt
    10141 ccccgcatag gataagcagg tgtggcaaga gtaacagcac cgccgcagta ttgaacaata
    10201 tttttcccgt agtgactgtg actcaggaca tgccctacac caaaaatcat agctctgccg
    10261 tcatgcccgg aaataatcag ctcttccgga gacagcgtta tcttataact ttccgctggc
    10321 aaagaagggt tatgtttcag acaaatcttt cgtacttttt catctcctga agacaaatcg
    10381 gtgctgtcgc tgcccgttcg tttttcaatc tcttccgaca gtacccgcaa agctgttttc
    10441 ataacaggcg ttgtttttgc tgcctgcaaa gaatggcctg ctcttgacga gctcagatag
    10501 actgtgtccg ctgctgcaaa gaaactcacg acacacagga aaaaacagct aataaaaaca
    10561 cgcacttttt gaacctcctg aaattttatt taaaaaagat gccagtaata tctacgctat
    10621 ttacttgtta ttttcgccag aatctataaa tagattgttg cctgacgatc ccgtgtagtg
    10681 cctgccgtgt agctgtgtcc cgggcgcagc gatcgctcgg caaaagaact gtcgctgagc
    10741 gcctgcgtct ttatttccca ccgattcctg tacacgtcga caatgcggaa ctcaacagga
    10801 tattccggca gcgcagaagt cgtcacatga tagatcccct gaaaccgctg gatgctgtta
    10861 gtgtggatat gcccggaaag aaccattttc attttcggga atgccgccaa ttcacgggaa
    10921 atcttttttc cattgtccag atttccgcca aatttataac cgggacggcg aaaacgatcg
    10981 ggaacaggta aaaagggata atgcgaaact gcacacgctg ttttttctgc tgttctttcc
    11041 aattccttta tcagccagag aaattgctct tcgggaagtg cccagcgcat attattcaga
    11101 tttgtctgct gcctttttgc cgcattttta tccgcctccg gagaaagaga gccatcgtta
    11161 tgaacccacc agggatccaa tgcaaaaaaa cgtatgtttt tgtgggcaaa ggaataatag
    11221 gtcttgctat ccggaagccg gtgcgcataa gcgtctgtaa accattttct ggattccagg
    11281 ctgtaacaat cgtgatttcc gccgagggga tagtagggga acccaagtga ttcaatcgca
    11341 tcacgggctt gcaggacggc ctcttccgta ttatggctga cgatatcgcc gctgacaatt
    11401 aaaaattccg gttgaagtcg ctgcaaatcc cttttcagac acgccatcac ctccggcatc
    11461 atggaacata ggaaacgatt attccacacg ccgtctctct cactggcaag gtgcggatct
    11521 gtaatatgga caaaacgcca catgcgctaa aatttctcct gctcttgcaa aaaaatgtca
    11581 acagacggag ttaaacccac attttacctg ataaggcctt ttattcgtta atgcgtcctt
    11641 tactcaggaa aaacagttaa aagaagagcg ctttgcgatt ttacatccaa gcttgaccgc
    11701 ttaatcttcg ggacgttctc cggcaggtgc cattttgacc agacgcggga aaaaacgcgc
    11761 cttaatggat gccagatctc tttgagccgc cattacttca tcgatatttt tatacgccat
    11821 gggggattca tcaagccccg ctgaaagaag tgttatcctg tcctttttca gcagatcatt
    11881 caactggtgc caggaaaaca gtttttttgc ctgtgtacgg ctcatgttgc ggcccgcccc
    11941 gtgggctgca ctgcaaagac ttaatggatt cccgagccca tgtacaacat aggcaggggc
    12001 tgccatggat cccgggataa tacctgcctg gccctcccat gccggagtag cacctttacg
    12061 gtgaacaatt agctgccgtc cctcatgcat ttctttccag gcaaaattat gatggttttc
    12121 aactttcagc agcacctgtg cacccagccg ctcagttatg tggtcatgaa tcaattcgtg
    12181 gttggctgca gcataccgcc ccatgagttc catggcatcc cagtattcgc gcccctcggt
    12241 ctgtttaaga tccagccagg caaggcgtga caattctccg ggcagacccg ggcaggagga
    12301 ctgcgcccgt gcgctataat atttgcagac ttctgcgccc gtaccccggc tgccactatg
    12361 gctcagtaaa gccagataac ttccgaggtt cacgccaaga caatcctcga aaacatccag
    12421 aatcccaaac tcaacaaaat gattgccgct gccgctcgtt cctaattgct cccaggcggt
    12481 gtctttcatt ttccgggtta caggggaaac agaccaattc tcatcaagaa ccttatgcag
    12541 gcgctttttt ttaaaagaag cgccgatacc gaaacgtgtt tcctgttcta tcgcctcttt
    12601 aagccgggta tcattgccat cgtcaagaag ggaagggggc agatccaaaa cggaaaggcg
    12661 catcctgcag gcaatatcca ctcctacggc ataagggatt acagcgttgt ccgtagcaag
    12721 cacaccgcca atcggaaggc cgtagccaac atgcgcatcg ggcataagcg cccctttaac
    12781 ggatatgggc aacgagcacg catctttcat ctgccttatg gcggcaggat caataccggt
    12841 gccccattgt gcaaaagaca gttctttccc cggagcacag acagtctcat atcgaacggt
    12901 gcctgcccct ttccccaata aagcgagagc caggggcgca aagagaggat ctctcattct
    12961 tttttgcggg ggaagcacag tctgacgaag cgttttttct atttttttag ctgtccagcc
    13021 ttcacgctgt gctccctgaa tcgcatccct tgccgctttg cacactgatt cacctgtaaa
    13081 accgaattta gccagtaatt tctgaatcat ggatacaggg tatcataaag cgccagaaga
    13141 gagcgatggg catcaaaggg aatatccgga cgttcatccc ggctaaaaaa acaaagctca
    13201 ctcacgtcgg aactggggga tagatctccg gaccatgcgg taatgacata cccgagcacc
    13261 aacacagacc ctttgtcggc actgcggctg ctgtgtgcgc ccagcagtac cggccctgag
    13321 ccttcaaggc cggtctcttc aagcatttcc cgcacagcgc actgctcgcc attttcaagc
    13381 agttccataa aacctccggg taatgcccat tttcccagac acggctcaac atttcgccgc
    13441 ccgaagagca gccggttttt ctcatcccgt ataaaaagac agcatgcagg gacagggttt
    13501 ctgtaaaaaa aacgattgca gggcgggcaa taaggtcgga cgctttcgcc gtcgtgctca
    13561 tcaacaaggg gagtgccgca ggtggcgcag taatgccact caaaaggcac cagcggtaaa
    13621 ccgttcggca ttataaaaaa ccttctttag atttaagtca ggtacttaca ccaacttttt
    13681 gcctgatatg aaaagataac aaacaaaaaa ccggttttgt gccctcctca cggagaagag
    13741 agacggatga gtcagactgt tttttcgcca cagcaatcag cgcagcagtc gcgcaatgag
    13801 gaaagccatc tccagcgcct gcgcaccatt taaacgcgga tcgcaggcag tctcatagtg
    13861 cgcttccaaa tcatgctgtg tcacattacc gccgaggcat tccgtaacct cttcaccggt
    13921 ctgctcaaaa tgtacaccgc ccagacagga atccaaagaa gcatgcacgg caaaactttc
    13981 ttccagttca ctgaaaatat tatgaacatg ccgggtcttt ctgccgtcag ccaagctttc
    14041 tgtattacca tgcatagggt cgcaacacca aagaaccggc aagtgtgctt ttcgaatcgc
    14101 attgataaga ggggggagca gtgcttttac ttttccggca ccgaagcggg tgattacggt
    14161 aatgcggccc ggaacatgag cgggattcag acattgaagt aaagacacga gttcttctgt
    14221 gtcggaagcg gcgcctactt taatccccac gggattggag attccccgaa ggtattccac
    14281 atgtgcaccc ttcaactggg acgtacgata ccccagccac aaataatgtg cgcccagatt
    14341 atagaaatgc tcttcgttct cctgacgggt cagggcggca tcatagggca gcagcagggc
    14401 ttcatgagag gtgtaaaaag caatgcgctt cagcctgtcg gcggcatctc cgcccagcat
    14461 atccataaaa cgcaccgcat cagcgatcgc atccagaata cggcgataat cttgattatc
    14521 ggcacactga tgcaggaaat gggctcccca tttttcagga tgatgcaaat cagcaaagcc
    14581 gccttcaatc agggcacgca gatagttcag gctcgcagca gcatggtgat acccctcaat
    14641 catccggcag ggatccgctt tccgcgcttt ttcatccggt gaaaaatcat taatcagatc
    14701 cccgcgatat accggcagtg aacgcccgtc gatgatctcc tcgtctgcac ttcgcggttt
    14761 tgcatattgc ccggcaagcc gtccgatccg gatgacaggc cgccgggtag cataggtcag
    14821 caccagactc atttgcagca atacctcgag ttttctgcgc aaagcctctg cggagcagtc
    14881 tgagaaccgc tccgcgcaat ccccgccatg gagcagaaaa cgcttgccgg ctgcggcttc
    14941 agccagctca tttaaaagcc gatctatttc tccccgtgac acaagaggag gctttgcgtg
    15001 cagaatttca agcacctccg ccacctgcgt tttatcgcca taagaaggct gctggagtgc
    15061 tggtttctcc tgccatgaat acggatgcca tttctttttc gaagactcta tatgcattga
    15121 tatggacttt cattgattaa aaaggtaata cggaaccggt ctcattaaat caaaaaagta
    15181 aagtctaaac caaaccggtt atttgtattt ctaagaacct taaatacgta catgtgaatc
    15241 cgtcaacggt cttctgataa gatttcagga acccgctacg cacggaatgt tttttgaaga
    15301 cccaaccaaa tgaaaaagag gtaggaatat gaacccagcc aaatgttctt tgaaaggttc
    15361 cgctgcatta tttctgctca ttttgagcat ttttcatggc tgcccaaccc ccgcctctgc
    15421 tgccgtcaac attgcccgga tcaaacccgg ggttcattta acggatgatg tgcttttacc
    15481 tgatcagatc tgttgtatgc gcgtttctgt cactggagaa gcttcacttt ctgacatgat
    15541 cctgaccccg ccgcccggag tttattattt tcatgccggc gatcatgtca tcttgtccgt
    15601 ttttgacagc ttgcaccata ctgtgcctat tttctgggat ggggcaggga aagaggcaac
    15661 ctgttttaaa gtacttactc ttcaggagca tttggaaatc gaattatctt ttgatcctga
    15721 gttgcctgtt ttcaaagaaa cgcctcccga tagcagggaa cagtggttcg atgtccctac
    15781 agactccacc atctacgctt ttgtgggtgg aggaaatctt tacgggtggt ttacgggata
    15841 tcgtcataaa gatcatggcg caggcattac agggcttgta cacactcatt acccgtggat
    15901 gcctgttttt gaccgcacaa atctgaattt tgaacacatt ttttcctccg gtgcagagaa
    15961 tatagagcgc gtttttttta cgccccgtac tgatcctatg cgtttttgta ttgaagcccc
    16021 gaacaagatt cgtgttcggt gggacaaaga atcgtccgcc tggggtgtgg actgtgaaat
    16081 gatctattgt ttttccggag aagatgccat cgatatttct ttttctttca caccggaaga
    16141 ctatcatgcc tcactggatt acctcggctt tatgtgggca tcctatatgc ataatgtcaa
    16201 agaaagaggt atctcttttt tttcaaaaac gccatcgggt atgaaatgga tcttgtttgc
    16261 gccggaaaat gacgcccata agaagaatgg caatgtcgtt ccagtcgaag gcttggaaag
    16321 caaatctcct gaaaacgctg aaccagcact gctgcttaaa tgtactgata cactcactgc
    16381 cctggttcct ttttactatg gcacatttta tccggacacg aatctccccg aatcttttgg
    16441 cacgatgcag tatgttatgt tgtttcaaaa aagcgaagga ctgcgctatg ctgtttataa
    16501 ttggggaccg gtgaagcatt gcagcacctg ggattggcaa tatctggtca aatcgcctca
    16561 ggcgggacaa cgctacgact atctgatgcg atgccttatc acaccggtga ttacagaaga
    16621 tgaaattgac ctggaaagaa aagtgtggtt gaaaagcctt tccggagatt cttctgaaga
    16681 aaagcctttg tttttatttc ctgaccttcc tctttactgg tcacctgcgt ctgttttttt
    16741 aggcttggct tttatggggc agcaaataga accctgggcc ccagatcacg ccttgacgtg
    16801 gtacagatat ctactgcaag aatcagaaaa caaacagccc ttttctatgg acatcgatcg
    16861 ctattatgtt cgttcgagca ataacgacgg gttaattcac gaatgggaaa catggataga
    16921 gtatgataat tccgatccgg ttttctggca gcatttggca atggcataca acacaaaagg
    16981 gaattttgag aaggcagatc actgtttttc tgaagcattg ctccgaaaaa aagacgatcc
    17041 gcacattctt ttacattatg gaataaataa tatctatata aaaaaaatcg aatatggctt
    17101 caaccaaatt aaagaagcgc ttaaactcga acccctactg gaatcagcga ttccaaccta
    17161 tctcaatcaa tgcggtgaat ggcttttcga gcaaaatgac ctgataactg ccattcagtt
    17221 ctacaaagaa tccgcaaggc tgtttccgga acactactgg tatatcgttc atctggcaga
    17281 gtgctacgag gcaatggggg atgacaggct ggcagcagaa ctctttgtaa atatgcttct
    17341 taaatttccg gagtcgccct acagtgcatt taagttggat aggattctaa aagataaaaa
    17401 cgacctaaaa gagcgaataa atatttggca ggaaatttac agacagcaca aaaacagttt
    17461 cattccagcg ctttatctgg gcaaggcact ttatcataag caggcatatc aggaggcact
    17521 gctcatagta gagcaggcat tggaagttat gcctgatgcg ccggaggcac tgctttatgc
    17581 agggctgtgc tgccttttat tgaacaatta tgatcaggga taccagaaga taaaagaggc
    17641 attgaagaac gatcccgtcc tctcaaccga agcgggaaca gaacttgccg ctgcagggga
    17701 tgcgctttac aagcagaacg acttcggcag ggcgctacag ctctttcagc tggcagaaga
    17761 attcgaatca accaatctct ggcatcaggt gcggcagggg gagtgtctgg aggcacaaca
    17821 taaattttct gatgccgccg ccctgtatac ggctgtgctg ctgaaagccc cggagtcgcc
    17881 ctacagtgca tttaagttgg ataggattct aaaagataaa aacgacctaa aagagcgaat
    17941 aaatatttgg caggaaattt acagacagca caaaaacagt ttcattccag cgctttatct
    18001 gggcaaggca ctttatcata agcaggcata tcaggaggca ctgctcatag tagagcaggc
    18061 attggaagtt atgcctgatg cgccggaggc actgctttat gcagggctgt gctgcctttt
    18121 attgaacaat tatgatcagg gataccagaa gataaaagag gcattgaaga acgatcccgt
    18181 cctctcaacc gaagcgggaa cagaacttgc cgctgcaggg gatgcgcttt acaagcagaa
    18241 cgacttcggc agggcgctac agctctttca gctggcagaa gaattcgaat caaccaatct
    18301 ctggcatcag gtgcggcagg gggagtgtct ggaggcacaa cataaatttt ctgatgccgc
    18361 cgccctgtat acggctgtgc tgctgaaagc cccggagtcg ccctacagtg catttaagtt
    18421 ggatagcatg ctgcaggacc acgaagacaa taaggtgcgg ataaaaagct ggcagcacat
    18481 ttatgagcat acaggcaaag cctcattgcc tgctttttat ctcggaatgt ctctcttcat
    18541 tgactgtcac tatgatcagg cgtatgcgtt attgtctgca gcagacagtg catcggaaga
    18601 aaacagcaga gcggtgcttt ataaagggtt atctcaggtc atattgggag aagtgcatga
    18661 gggcacggaa aacatcgaag ccgcattaaa acaagacaac acactgtcgc atgaagccgg
    18721 agaacgcctg tcagtgctgg ggcaccatgc ctttaatgaa gggaaatata aagaggctgt
    18781 ccgctggtat caacaggctt atttatatga acctgacgcg ctctggcacc tttatcatga
    18841 ggcaaaaagt ttggtcaaat gcaatgcacg gtctgacgcg ctcacaattt ataaaacaat
    18901 ccttgaaaaa gcgcccgaat ctcccgaatc ggcacaggaa atggacacct tactcataga
    18961 aatggaagag cctgcaaaaa gagttgagct ctggagaata ttgcatggaa aagcggagaa
    19021 ggcactggtg cccatccttt attatgcagc tgctctttgt gacaccggcg aggaagagca
    19081 ggcaatagag ctcatcacag acttctttca ctttagagat acgagcggat tttcaaccga
    19141 cactctgcct gataacgacg ggagcaaccc tctcccggaa gaaaacgccg tgctggacaa
    19201 gggaaaaact cttgtcatcg agcaactttt gcacttctca catctcgcca aaagaaccgg
    19261 acataacgaa ctggctttct ttttgcaaca caaggcagat caaggccgta cagggcaggg
    19321 tgattcgaca ctatccctgc ttgaatggct caaccagtta agggaatagc attcaaattt
    19381 ctgtctcttc tttccggttg cttttatcaa gccccatagt aatccagaag attttaccca
    19441 gcatgggcaa agaaacggtg tagaccagtg catgaagcca gctgcgatct cctgccttgc
    19501 catgaataaa gaggagacag agcagagaag ccataaaact ggaaagtccg gataaaataa
    19561 agcacaggcg aaatccgagc aaaccgaaat gctcaatcag aaatccggcg acaagcggcg
    19621 tgattcccag tgccagcgcg gtgcagatcg tccaaagaga ggtgtatccg attctcgatt
    19681 catccggaac cagattcaac atggcgcggt tggctgccac attaaagggc gcattaaaga
    19741 ctcctgcaag aacaactgct gccaatgctg cggctgtgcc gcccttcatg ccgggcaaga
    19801 gaaacaaaag tatcagagga gcaaagctgt gagcgaacat ggttttcgcc atcgcgagtc
    19861 cgctgccgtt tttgtcagta aaccgccccc agggtcctat ggtgagaaaa ataccaagcc
    19921 cgctgcacgc tgtcagcgtc atgttcaaac tatccgtcag tccaatgaca tcgcgtaaat
    19981 agaggacata ggaagcgctg aaccagacaa gcccggaaaa agcaaaggtg gatatgagaa
    20041 gaaaacgtct gaacaccgga tcacaggctg cttttttata agcactcatc cctgtaagcg
    20101 cattaatttc tacagttcct tcacccccgg ggatgcgccc cattgtgatg agactgacaa
    20161 tacccatggc tatcccgatt ccataaatca ataaaaatcg gggaatatcc ggatttcctg
    20221 ccagtagccg ggcctgcaaa aaaagcacag ccacattgat gagctgtgtc atggctgtct
    20281 cggtgctgaa ataggtagac cgtttttcgg tcggcacaat ttcgtgaagc cagggaagcc
    20341 atccaccggc acccagggcg cgcatgacac aaaaaccgag tatggacaca agcaatacag
    20401 cccaggatag ccatttgacc tgagaagcca aagccaaggg cattaaaaat accagacacg
    20461 tgataatgtt tctgagaaac caggaggtca tcatgacccg tttgggtccg atgcgtatca
    20521 gcattggcag cgtaaaaagc agaagcagac tggtgaaggg catgaaagag agcagcagcc
    20581 caatcaggct ttccggcatc tgaatctttc tgccgaataa gatcattacg ggtccgatga
    20641 tgttctgcca cgaaacaaca ttaatggcaa caaacatcaa aaatcgctgc tgttcctttt
    20701 ccagcctttt aaaatctgta agaagcggca cttgcataaa gaaagggata cttttctatc
    20761 gggcggtcac atgacccggt tagagaaaac ttaacaattg ttgcgatgaa gtgagttcag
    20821 actttaatgt ccgcaacact ttcaagaacc tgagtcggca taaaaggaaa agcgctcagc
    20881 tcctcgcggc gtgtgactcc gcttaaaacc agaatcgtct ccatacccgc ctgcatgcct
    20941 gcgataatgt cggtatccat gcggtcgccc accataacgg tattctgaga atggacgccc
    21001 agatagttca tggcggagcg catcataaac ggggcaggct ttcccacaaa aaaaggagca
    21061 cgcccggtcg ctttttcaat taaagcagcc atggcgccgc atgcgggaac aataccgctt
    21121 tcggtgggac cggaagcatc cggattggtt gctataaatc gtgagccctc ggcaacaagg
    21181 cgcacggctt ttgtaatctg ctcaagattg tacgccattg tttctccaag aacaacataa
    21241 tccggatgat ggtcggttat aatatagcca ataccgtgca gagcactagt cagtccgctc
    21301 tcgccgatga caaacgccgt tccgtaagga cgttgtccgt gaagaaactg cgcagttgcc
    21361 atcgctgagg tgaaaatatt ttcaaccggt atgtccagcc cgctcgcacg aaggcgataa
    21421 gcaagatcag caggagaaaa catgggatta ttggtaagaa ccaggaattt tgcttctttt
    21481 tctttgagac gattaacaaa agcctgtgcg ccgggcacag gcaaacgacc ttttaccagc
    21541 acgccgtcca tatctatcag atagttttta ggtgaatcca tggttttctc ccggaaaata
    21601 attgcattgc tactgagtgg aaggaactgc aatcaggaag cctgagtcat gccggcacga
    21661 ggattttttt aaagacctca atagcacggc tcagcagtgc atctgaaata tccctgtgaa
    21721 atacgacacg gatgcggtca tccctgtaag ccagagcccg cacacctgcc tcttccagtt
    21781 gtttcacttt cagcgctgcg ttcgctgtct ggatataaag gatattggta ggggagggca
    21841 gggcaaatac aaccccttca ttttcaagag cacgcctgca ttcacgcgca cgccgatgat
    21901 cttctgcgag gtcggacaaa tgatgttcca acgcgtataa ccctgcggct gccaatacac
    21961 ctgcctgacg cataccaccg cccagcatct tgcgaaagcg aagggctttc tttatgactt
    22021 cggtgctgcc ggtcagaagt gagcctgccg gtgcccccag tccttttgac aggcagaagg
    22081 aaagagtatc acagcacctg ccataataac gggcctgaat attcgtttct atacaggcat
    22141 gaaaaatccg ggcgccgtca caatgaaggc gcatgctgct ttcctgacac acacggaaaa
    22201 cagcctccag ttcatgataa tcccagcagg cgcctccgcc ccgattggtg gtgttttcca
    22261 cagagaccat agtagttttt gataaatgcg gatcgtcttc ggaaacaatt tgttcttgca
    22321 cctgctccgc agaaaacttt cccagcgggt cggggatagt acgtacaagc agcccgccaa
    22381 tcatggcgag gttcgcattt tcataatgaa aaggatgtgc tgattcgctg agaatcactg
    22441 tatcgcctgg acgggtttgc gccagaaatg ccacaagatt cgccatagta ccggaaggca
    22501 gaaaaagggc agattctttt ccgatcagtt ctgccgcttt gctttccagc gcccgcactg
    22561 tcggatcttc gccataaaca tcatcgccca cctccgcatc agccattgct tttcgcattg
    22621 ccggagaagg gcacgtaaat gtgtcactgc gaagttctat catgtccata cagatcgcat
    22681 ctcattattt tacagcgatt ttaccgtcag ccgtttcata cttctgaccg gcactcatgg
    22741 attggacacg tgcctgaaaa aagctttcct gaacagcgcc caatgatttt cgctgccagt
    22801 ttgaagcctt gcagagccct tcatacaaag tccagcggtt ggcattttca ctcataacca
    22861 gtaaagcgtt ctggtcgcgc tgttttttac ttgtctgcaa tttgtagtcc cgggttcgta
    22921 atatgcagat agtcccctga gcctgttcat aggcatggcc tgaattcaac agagcctgaa
    22981 tgagctcgga ccttgcggca cgggtatgta ttgcctgcag aatttcttca ttcgatgaag
    23041 ccacgccgtc atatccatca aaagttgacg tgcggtttcc ttccagaact gactgctcaa
    23101 ctgccagggt aaaagcattc agtgatgctt cgggcacatt agtgtagtcg gcacgcatgg
    23161 tactgaaggg gtttgtaagc ctggcacagg ctgtaagact tacaagaaga gccaacaaca
    23221 gataaccgga ttttctcatg ggataatcct tccgcaggtg agtgcgcctc gggcgccgtt
    23281 aaaaaacaca cagaaatgtc agagattctc aagcagaatt cgcgctggcg atgggaagca
    23341 tgcaacacac agctcagtat aagcaaaatc agccatagag ttattttttc gaaacaaaga
    23401 tgaaagacgt cgctggcatg gttcccggcg gtataaatct gtctctttca tgatgttacc
    23461 tgcgaaaaac agatttcctt cccgcctgac cgcattatct ccgtataaca agcgaattgt
    23521 caaatctatg ccatttcgtt tcataaaaca gccatcgatc gcaacactgt gacgatctgc
    23581 ctttccctgc ccaaattttg tattatagag agcgggtctt attcaggccg gtagggtcaa
    23641 atagtgctct aacatgttgt gatataggga accctgtgcc catgacttct gaaacgcaca
    23701 ttgaaatagc tcgtattgct ctcttttccg gcgacagaaa agagagcaaa ttactgtccg
    23761 acgaactcac cacactgcgc cctgcctggc agatcattac ccggccacaa ttaaaagact
    23821 ttgactggac acatattcag agtgaaatcg actgccttct tgttaaagca gactctgaaa
    23881 aaaaagccct gtctctcctg gaagcgataa caggtcataa aaaagctgaa ttgcctcctg
    23941 tgatcgtcgt cacgtccctc tcaaaaaaac aaaaagtccg cgaggcagta aactctttca
    24001 atctatcccg cctctatgtg cttctggacg atagcgatac catcatttcc gcgttggaat
    24061 ccatcatgcg cgcctaccgg gttgaacaga agcttcgtca agtcaacacc ctgctctcga
    24121 atcttattga agaacgctca aagacactcc acgaattcag tgaaaggatt cgttttttaa
    24181 ctgagttcag cagcgatgcc gttttcgaaa tagaaaaaga ccgaactacc ggaatcagca
    24241 cttttatcag tgcaaacaga acggcgtgca gctggctggg gtatagccag gaagagctgt
    24301 tgcaactgcc ctttagccat tttctggaac cggctgccat tacaaacatt acacaactcc
    24361 tgaatacaat ctctcagccg caggaatttt ctttagaact gtcccttaaa aaccggaacc
    24421 aggaacggct ttttcttaat atagtgatcc gtgcttttga gagtgacaga gggctgcgca
    24481 tgatcgctat tgcgcacaaa atactgtcgc ctgaattcag cctatcggag cacgcctccg
    24541 aaagtgacta cgagcaggta gtgtcgcaca tcggccagct tatgtatatc ggcaatgtcg
    24601 atacccagca gatgaagtgg agcggcgctg cttctcagat caccggatac acaatcaagc
    24661 acctgaacaa gggcagcatg cgcgagtggc gcaagcgaat agtatcagaa gattacccgc
    24721 gcaccattgc agcaataaac aaagccattc tccgcatttc ctcatacgaa gtggaatacg
    24781 gtttcttgca caaaaacggc gagatacgtt atcttgaaga caggggagtc gtgctgccgg
    24841 atcatcgggg tgctcctcat cggatcatgg gtaccatcaa ggatattaca gagaagaaga
    24901 tggaggaaga agcacggcag catctggaaa aagaattaca gcacacacag cggctggaaa
    24961 gcctcggggt tcttgccgga ggtattgccc atgattttaa taatattctc gcaggaatta
    25021 tcgggctgac agatcttgca cttcgcgaaa tcccgtccga ctctttcgct ttcgaagatt
    25081 tgaatgaggt gcttcatgca gcgcacaggg ccaaagaact ggtgcagcaa attcttgctt
    25141 tcagtcggca gggtgaccac gagcgcagtc tgatcttccc tcatattatc gcccgggagg
    25201 ttattcgtct tttacgtgcg tccttgccgc cggccataga aattatcgac agtatcgata
    25261 tccaatcggg tgcagtaatg gcaaatgcct ctcaactgca tcaggtaatt accaacttca
    25321 gtacaaatgc ggcgcagtct atcaaaggtg gatccggaaa agttgaaatt caggtctgcg
    25381 atgttgaggt agagaattct ttttccgcta aaaacccgaa catcccgccc ggttcctatg
    25441 tgaggatcgc tgtcagcgat accggacacg ggatgactcc ttccatcctt gcccgggtct
    25501 ttgatccttt ttttacaaca aaaggtccgg gagaaggtac gggcatgggg cttgccgtcg
    25561 cccacggtat tattacagat catggcgggg taatcctcgc tcaaagccgt ctgaaaaaag
    25621 gatccacttt tgaggcctat cttccgaaaa taaccgaccc tcaaataaca gaatcacttg
    25681 tccaaagcgg atcagcagac ggcagaaata atacgcatat ccttttcgtg gatgatgatg
    25741 agacagtgct gcgctttgcc tcttccgctt taccacggct gggctttaag attacattat
    25801 gcaaaaatgc ggaagaagca ttacatgcat ttgcggaaaa taccgatacg tttgatctcg
    25861 tgattactga tcagagaatg cctggaatgt ccgggacaga attggctgcc gctatcagaa
    25921 aatcatcccc tcgaatgcct gtaattctgt ttaccggttt cagcaaaaca ctgacagaaa
    25981 aagaccagaa aatcaccggc attcagaccg tgctcaggaa gcctattatt gtgcgtgacc
    26041 tgctgacagc tatccgtaaa acgctgaaaa gcccgaccga cctttaataa tacacactcc
    26101 aacagtaaag ggaacttttt gtgaaaataa tgattaccgg aggcggcaca ggagggcata
    26161 cctctcccgc tttggcagtg atagaagaac ttcgcaaccg cgatccacgc ctccttttgc
    26221 agtgggtggg aaaaaaaggg agcatcgaag aacgtattgc caaacgggaa ggaattcctt
    26281 ttcgatccat acctatggaa ggctggacca gaaagatttc cttgcgttta atttgggtag
    26341 gccttaaatt tctttattcc gtgttaaaag cctggactca aatacgtgtt tttcggcctg
    26401 atgctgtttt cggagttgga gggtatgtca gccttccggt cttgctggtt gcgcctcgca
    26461 tcggcattcc agcattttcg cacgaacaga ataaaaggct tggtctggca aatcgtcttt
    26521 cagcgaaaaa acttacaaca ctatttttga gctatgagga cacactgggc gtgcctcctg
    26581 aaacacccac aaaagtaaca ggaaatccgg tgcgaagcgc tttttacaat ccatccggaa
    26641 aagaggaatg ccgtgaggcg ctgggtctgg ataaagaagt gcctgttatt ctagtggtag
    26701 gtggcagcca gggcgcccag acattgaatc gggcaatgca ggaagcagtg tttaatttca
    26761 gtgccaatga tgcgcagttc atctggtcaa cgggtcccgg ggatctggcg gactgccagg
    26821 cactggcaga aaaagcaccg tgtactgttc aggtttatcc ctacattgaa aatatggctc
    26881 agacctgtgg tgccgccgat cttctgattt ccagagcagg tgcttccacc acggcagaaa
    26941 tcgctattat gggcaaagcg tccattctgg ttccttatcc ctatgcaacg gataaccacc
    27001 aggaacacaa cgcacgtgct tttgaagcag caggcgccgc acaggtgctc cttgacaaaa
    27061 attgcactgg cgagagcctg acacagcaaa tcaaagcaat tattcagaat ccctcagtgc
    27121 tggcttccat gggaaaagcc gcggcagacc tcgccagacc tgctgcagcc gatgacattg
    27181 tagagactat tctcgaaaaa gcctgcacgc ctgcagacta ttaggcgaag cgatctctga
    27241 ccagtatttc ctcaagcggg aggtggccgc ctctgggatt ggcgggtgca atggttttgc
    27301 ctacggcaat cataagagca atcacatgat cgtgtggaag gttgatcagg cggccgaccg
    27361 cttcaaaatc aaaaccgacc atggggcagc tgcccagtcc cattgacttt gctgtcagca
    27421 taagggtctg tgccgccata ccacatgaac gcatggcttc gtcacgctgc acctgatctt
    27481 ttccccgata ataggcatca atagcaggga ggataatatc ctggtactcg gaactgctgt
    27541 tgcgccaata acggtgaggc tctttttccc aggccttcac atcggcacac aggataatga
    27601 gcagggaaga atcagtcacc tgcacctgat cccaggcgac ttttcgcaat tcctgacgta
    27661 attgcggatc ggagacaaga acgaaacgcc agtgctggat attaaaggca gtaggggaga
    27721 ggcgaaccga tgaaaagaga tcctgaatca tttcttcaga catttgaaag tctttatcaa
    27781 atttcttaaa agcgcggcgc tcttcaattg cttgttttat atgcatgtct ataattcctt
    27841 ttgtagactg cccaacacgg cacaagccgc ttttcttccc ggcaccgata tatacggtct
    27901 tcactattca cttcgtgaag attgggactg tttcttttga atgtgcgtgc gtagctgctc
    27961 tacccgtgat tgtgcctgct gatgactggg atccaaagcc agaaccgctt ccatgaactg
    28021 cagtgctttg gtgtagttcc gctggcgaat aaggcggata cctagattat aacgcgcagc
    28081 acataccttt ttcttaaggc ggctgttttc ctgatcattt ttaaatagat cacgatagat
    28141 ccggaaagca tccccatatt tccctgatcg gaataaataa tcaccctgac attccagatc
    28201 actcatttca ggcttgaaat gcgtcgcttt gtatttctgg taggcatttt cctgctcgcg
    28261 ggtataaaga tcagcatcct gaattgagac aactgcacta cgctcaacca gcaccataag
    28321 attattgctc ttctgatctt cgggcaccag aaaaaagcgc acttcttcat tccaccgtgc
    28381 atgcatgggg cggcctacaa tcacttcgcc atcgctgaat tctactgcca gcgcttttgc
    28441 cggtggaata tcctgtggaa attcaatggc agggtcaaaa cgcccatcaa aacttttaac
    28501 atagaaaagg gcttttacgt ttttgaaggc aataggaagg gtcttgttta aagaaatgcc
    28561 ctccgcagtc accaactcca gatgaaagat ttctgcctgc ctgttcaggg caaaagttga
    28621 accgtaatat acgtcccccg atttcattcg gacaacaact ttttgccttt cttttttgta
    28681 gatggaaggc gtcggaatgg actcggcgga tttttccttg gatcgggacg aaaaacgatc
    28741 aagcatctcc atatactcca tcgttgtcac actaaacccg aagatccttt gcagctacca
    28801 gtatagagca tccggaaaga aaaggcaaca aatctcgttt ttctcaagaa aacgcaaaat
    28861 tgggcgtgat atttcttttt gagacggcaa gccaaaacgg atatactgaa ggggttagga
    28921 cacggcgcat ggaggcttta ccgtgcagaa tgcctatgtt ctggttttaa ataaatcctg
    28981 ggttgcgatt caggttacct cagccaggcg tgcattgcgt ctcctggtgc agggaagagc
    29041 ctgcgttgtg catcctaccg attacatgct gtacgacatg ccctcctggc tggaattatc
    29101 catgcatgca gacggcattc ctccggatcg ttacgtctac accactgctc agcgcattcg
    29161 ccttccggaa gttgtagttt taaacgtttt caacggattc catgttcagg aagtgcgatt
    29221 gtcccgaaga aatctttttg cccgtgacaa agacacatgc cagtattgtg gcagaacagg
    29281 caatcgctcc tgcatgacca ttgatcatgt aattcccaag tccagaggcg gtgaagacag
    29341 ttggagtaat ctggttgtgg catgccctgc ctgcaatctg aaaaagcggg accgcactcc
    29401 tgatgaggcg cgcatgccac tcctcaaaaa acctgtagca ccccgatggc tcccccgttt
    29461 caccagaccg cttttgcgtg aagagctcat ttcctggcaa cgttttgttg atcttgctta
    29521 ttggggttca gactactatt tttcctgaaa caacctttgc tgcactggca cacaatttat
    29581 taaatccatg gactttatca gatatttaca ttaccttcgc atagaacgca atttctccgt
    29641 acacaccctg cgcgcttatg ccaatgatat cgccactttt tgtgatttta ttgaacatgg
    29701 tgttgccgca ctgcaccgaa aatccgacga tatccgaccg cctgcatcac tgagtgtgct
    29761 gcagcgggca gatcgaaata ctgtccgggc atttctggcg cacatccaga cttcaggtgc
    29821 aaccgcacgg acggcagcac gaaaactcgc agccttgcgc tctgtgtatt tgtactatca
    29881 gaaaaccggg caaatcacaa cgaatcctac agaggggctt cgctcaccga aacgtgctca
    29941 aaacctgcct gaagttttgt ccatcccgga aatcacggct ttgctggaat cagttgatac
    30001 tgccacagca ctaggatccc gggatcgagc cgtactggaa gtgctctact catgcggctt
    30061 acgtgcggca gagctggcag gactgacatt tgaggatatt ttctggcatg atgagactat
    30121 gcgaatcttc gggaagcgga aaaaagaacg tctcgtgcct gttggagggc ctgcactgag
    30181 agcactcaaa tcttatctgg gacggcgtgg tgagctctgc agccaggcgc agcccattgt
    30241 ctttgtgaat catcgcggcg gcgctttaac cacacggagc atccagcgca ttgttgaagc
    30301 acacgttcaa aaaagcctgc ccgaacgcgt cggcgtttcg ccccacaccc ttcgccatag
    30361 tttcgcaacc catatgctca atgccggtgc cgacctgagg gtagtacagg aaattctggg
    30421 gcacgagagc ctgtccagca cacagatcta tacgcacgta agcattgacc gtctaaaaga
    30481 agtgtaccga aacgcacacc cgcatgctta gtaatcgtac ttgcctcttg gcttattcac
    30541 ccatggcttt aatgatgagc cgtttaggac gtctcccgtc aaattctgcg taataaatct
    30601 gctgccaggg cccgaggtcg agtcgccctt ttgttaccgg aacgatgacc tcgtgatgta
    30661 caagcaggct tttcagatgc gcatcgccgt tggtttcgcc tgtccgatga tgcctgtaac
    30721 cttctttgaa aggtgcaagc gattcaagcc actcgtctat atcagcaatc agcccggact
    30781 ctgcgtcatt cacatacacg ccggcggtaa tatgcatggc ggatacaaga atcatccctt
    30841 cctgaatacc gctgcggtgc agtgcctgct ccactttatc ggtgatatta atgtactctc
    30901 tttttttggt agtctgaaaa acaagatact ctgtctcaaa tttcattctg cgccctttcc
    30961 cgcaaagata gcctgtaggc tgctgcagct gatgtgtact ttcgaaacaa ttcagtgtac
    31021 catactcacg ttgtcggatc agccctgccc ggaaaagtga tttcggtttc cattgcttta
    31081 ccgctttttt gactggaagg aaggcatcag actatgcaac aggaattccc gcagaagcca
    31141 agttgattta attcagataa aacggtactc ttttatagat gtctgttttt tcagttttgg
    31201 acagtacatc cttttactgt gaccggcatt ctgtgtgata ccggcgtgga agcactatta
    31261 caactccaac ggagggaaag gctatggcca aaaaaacagt gaatgtggca atgatcggtg
    31321 ccagtttcat gggaaaagcg cacagcaacg cctggcgcaa agtagacatg ttctttgatg
    31381 cacccgtaaa gcccgtcatg aaagtcatgt gcggaaaatt cgaggaagag ctggcgacag
    31441 ccgatcgctg gggatggcag gaaacttctt tggactggaa agaagtagtg aatcgcccgg
    31501 atatcgatat catagatatc tgcacaccaa atttcctaca cccgattatt gccatagagg
    31561 cagccaaggc aggcaagcac attgtctgtg aaaaacctct tgcaaatacg ctagccgaag
    31621 ccaaggatat gttaaaagcg gcggacaaag ccgggatcaa gcatatgtgc ggtttttcat
    31681 accgttttgc cccggcagtg cagaccatga aaaaaatgat ttcctccggc gcactgggtg
    31741 aaattttcca cttccgcgcc gcctatcttc aggactggat tgtggatcct gatttcccca
    31801 tggtctggcg gatgaagaaa aaacataccg gttccggtgc tctcggcgat attggcgccc
    31861 acataaccga cctgtgccat ttcctggtcg gacccgtcgg cgaagtgaca agtgctctgg
    31921 aaacttttat caaaaaacgt gtggtggcag attccgacgt tggtgcctgg gatgccaaag
    31981 gcaacaaaaa gaccaaacag tacgacacag tcgatgtgga cgatgccgct gtctttatcg
    32041 gacggatcaa agggcagaac acactggcaa gttttgaagc tacccgcttt gctccgggac
    32101 gccgcaactt caactccatt gaaatttacg gcagtaaagg ctctctcatc tggaatcagg
    32161 aagacatgaa cgaattccag tatttcaacc gcgccgatcc cgatacgctt cagggattcc
    32221 gcaaagtact cgcctgcgac agcgggcatc cttatgtcaa ttcctggtgg cctccgggac
    32281 acattatcgg atatgaacat ctttttgttc atgaaatcta cgagttcctt tgcggtctca
    32341 agaagaaaaa gatcaactac ccgaccttcg aagatgcggt aaactgccag gctgttctgg
    32401 aagcggtgga aaaagcggcg tctacacgca aatgggaaaa agtttgaaaa ccaaccccgg
    32461 actgcaccta tctgaaacgg tgcttcaaaa gttataaagg agatgcttta tgaaacttgg
    32521 agttttaact gcccttttca gcgacaaacc ccttaaagaa gtcctggatc tgattcgtcc
    32581 gctggagctg cagacagtgg aacttggcac ggggaactac cccggcgagc cgcattgccc
    32641 cctgcaggaa ctgctcgaat ccaagccgaa aagagatgag ctgcttgcca cgttgaaggg
    32701 agaaggtctg gaaatcagtg cgctgagctg ccatggaaat ccgattcacc ccgacgaagc
    32761 atttgcaaaa gcaaaccatg ctgttaccat gaacaccatt aaactggcga atctgctggg
    32821 cgtcggtgtc gtcaacggct tctcaggctg cccgggatcc ggtcccggcg ataaaaagcc
    32881 taactgggta acctgtgcct ggccgcccga ttatcttgaa attctggact ggcagtggaa
    32941 cgaagtagtg ctgccgtact ggtcaaagat gaacacagaa ctcggaaatg caggcgttaa
    33001 atttgccttt gaaatgcatc cgggctttgt cgtttacaac acggatacca tgctgcgtgt
    33061 acgtcgggaa tgcggtgata acatcggtgc gaacttcgat cccagccatc ttttctggca
    33121 gggcatggag ccggtcgagt gcgtgaaagc cctcaaaggc tgcatctatc acgttcacgc
    33181 caaagactct atcgttcatc agaccaacgc cgccgtcaat ggcgtcaatg actacaaatc
    33241 ctacggtgat gaaattaacc gctcctggat cttccgcacc tgcggttacg gtcacgacct
    33301 gaaatggtgg aatgatttct tttccacgct gcgcatggtc ggctacgacg gcgcaatcag
    33361 cattgagcac gaagacagcc tcatgtccaa ctgggaaggg ctgaccaaag cagtttcttt
    33421 cctgaagaag agcatcattt ttgaagcgcc cacagccatg acctgggcgt aattttattc
    33481 cctcgttttc tagttgcggc cgggtgtcag tgacgcccgg ccgtctttta acagggagct
    33541 tttcaaaacg catgaaaata tatcttctgg cgataccgct tatcgccgca gtcagtacgt
    33601 tgttctatct tatttttcgt gagatggcgc gtgcctggct tcaccatcgc ctgaggatgc
    33661 tgctgttgga atgggcagaa aagaaacctg gcttttccat gtcctctgag gagcttaggg
    33721 aactgctcga agaaatgcct gctccgtcta tccagacccc taaaactgat ctcactgtca
    33781 caggtcttta tcttgccctc atcggtcttt tttttgtaat cgcttatgct ttaatcggaa
    33841 aaagccagtg ggcggtaggt gcctatttcg gaggcgttgc ctgtgtggtt cttggcttta
    33901 tcttaactac gataggcatt attctccgct ttctgcgcaa gcctccttta taagattttc
    33961 tgtttctttc tctctttttt gcattgtttt atatgatgga tacctctgta catagacatc
    34021 gttattccgg agatgcctgt aaggaaccac ttcgggtcgc cgtgatcggc agcggaccgg
    34081 caggcttttt ctgcaccgat tatctgttgc gcctggcgca gccttgtgtc gtgcatatgt
    34141 atgaaagatg gcctgttcct tatggctccg cacgccatgc catcgcacct gaccggcagc
    34201 gtattcgtgc tgtaactacc gctttcgacc gtattgcttc ccgtcagggc ttccattttt
    34261 tcgggaatgt aacagtaggt aaagacatct ctcttcaatt gctgcaacag cattatcatg
    34321 cggttattct ggcgacagga gcagacgacg gtctagtact gaacattccc ggagaagagg
    34381 tgccaggctt ctttcatata acaaagttcg caggctggta caacggaagg ccggattgcg
    34441 cctttctgcg ccccgacctg actcacacgg ctgccgcggt gatcggtgcc ggcaatgctg
    34501 cgctggatgc cgtccggatt ctgtcgcttc cttccgactg gcttccaaaa acagatatac
    34561 ccgagcctat gcagcaggca cttgcctcaa gtgctatcag agaaattcat ctcatcattc
    34621 gtcggggccc tctggatgtt cgtttttctc tttccgaatt tgcagcgctt tttgacattc
    34681 ccggtgtgag ttaccatctt catccggaaa actgcctgga aaacatacag gcggtttccc
    34741 ccgaagagga acagttgctt tctctgtata aatccgcaca tacaacgccg aagactgcca
    34801 ggcatattca cttccatttt tattcaaccc cgaaagaaat acagggcaag gaagccgtct
    34861 cggcactggt tgtgaagagc ggggaagagg acaccgcaat tccctgttat ttgattatca
    34921 gcgctatcgg acagggagct acggaaattg acggcattgc gctgaatgcg caaaaaagta
    34981 tgatccccca taaggccggg cgggtaacac aacagggtat tattgtgccc ggtctttaca
    35041 ccgccggatg ggcaaagcgg agcggcacag gcatggtggg gaaaaacaag cccgattgcc
    35101 gtgaaactgt tcaggctatc atggacgaca gggactctct tcaatgcaaa attgaggctg
    35161 tgcccgatct ccctaaagat gcagtcacgc tggatggatg gcaccgtata gatgccgctg
    35221 agcgtggtcg ggcggaaaac acaacaaaaa caagagaatg cttcctgact gtggaagaaa
    35281 tgctccaggc tgccggtatc aaaagcaaat tgccctctct ctgattaatt gagcgttgcc
    35341 agaagtgcct caacagagcc accgcttttt ttaacttccc tcaagtagtc agtcatagcg
    35401 tctgccacag agtagtcagc aatcggcggt gttttaggaa gtgttgccag cggggaatct
    35461 ggttcggcgc attcacccat aaggttttcc cactggtaca gtgggatggc aacctgatag
    35521 tttttcgcca tctcgagatc gctgaccaga ttccatttgc ctgcttcgtc agctttttcc
    35581 attttacctg aaaagccata ccacgccgtc ttgccctgac gtgcattgct caaacaattc
    35641 agatagaccc ggatctgttc tccgcttaat tcggttagca ccacctgatc gcctcgctga
    35701 cctgccgact gaaaaatgga attcgccgtt atagcacctt cccgctggtt gctccgcatg
    35761 acaaaggtat ggcaaaaggc aatatctgcc ccggctttcc attgcagtgc ggccgacgca
    35821 attcgcacca ggtcgggaat ctggatcacc tgacccgttt cgcctacaat ctcacccgcc
    35881 ccgggaaaaa tgcgttcttc ttccgcgtcc agccaggcaa tcatctcctg atcgggcgtc
    35941 acagtttcag gatccatgga gacaagcctg ctttctgctg tgattgcctt tttctgagcg
    36001 ggatcaaccg ttaaatcaag cactcccaca tattgggcat attgaccggc ctgcacaatc
    36061 aatgcgcccg tttccggcac cagagcagct tcatggagcg cctcatgggt atgcccggaa
    36121 acaaacacat caatagccgg cgccttttca gacagaagaa tgcattcctt tctgccgatg
    36181 tgcacaactg ccaccgtaag atcgatctga ttttccagcc gttccgcttc cagccgcaat
    36241 tgctcaccgc acttgtctat ggatttgaac gccccgcctg cgagggtagt aagaccgatc
    36301 agagcaactt tcaacccatt gcattcaacg attgtggaag gcgcaaagcg cggactccca
    36361 gtgtcatcca gactgttaag gcacaaaatc ggcattcccg caatttcgct gcattcccgc
    36421 aaatgatcat atccgtgaaa attatcgtga ttacccatgg cgcccgctgt atagccgatt
    36481 ttcttcatgg cttcgtaaag aatccgatgc tccgtttttt cagcaatcag atcccctttt
    36541 cgcatgacat cgccaccgtc aagaagaagc agatcgcctc tttgcgcacg catctcttta
    36601 acgaatgcgg atacataagg gagcccgccc tgaccgagtg ggtctgcttt cacgtgcccg
    36661 tgaatatcat tggtatgcaa aatgaggagc gagtgcgtat cggcaaaagc aaaagaaaca
    36721 caaagacaga taaaaagagc gaacactttg ggtatcgtgc gcatgaatta gatctcctta
    36781 aatactcaca gcattatgtc gtcgggatcc atgggctcgg ttgtgcgcag gtcctcagga
    36841 agcgcatctg caggccagct ttccatgggc aaaacagcaa gcggatgaaa gggacaatca
    36901 aaacttgcca agccgctgct gcggtcgata agaacagcca ctccggcaat ttcagcaccg
    36961 cgacggcgca gatgatcaat tgtcttcttt actgaaccac cggtcgtcac aatatcttcc
    37021 acgaccagca cccgttcgcc ttctttgatt ttaaaccctc ttttcatcgc cataccgcca
    37081 gcgccgtctt tttcgctata ggcggcacgg cactttaaat gccgcgccgt ctcgtaggca
    37141 atgacaatgc cgcctgttgc aggcccgacg accagatcaa tgtctttatc ctgaaacgcc
    37201 gctgcaagag cctgtccgaa agcctctcca tattcaggat actgaaaaac acgggctgcc
    37261 tgaagaaact gccgcccgtg ccgtccgctt gcataaatga aatgcccttc taaaagagca
    37321 ccggattctt taaaaatacg aatgatttct tgcgggttca tgaattcagt tcctctaaaa
    37381 ttagtgatac agcttcagaa ggataaggat gtttcaggat gggccggccg acgaccacca
    37441 tggaagcacc tgcctgcgcc gctgctcggg gcgtcatcac acgcgcctga tcgtcgcgtg
    37501 atgcccattc ggggcggact ccaggcgtca cgatcaatgg ctctgcaccc aaaacttctc
    37561 ttgttatgcc cagttccaga ggggagcaga caatcccatg ggcacccgct ttcacagcct
    37621 gtgaagcaag gcgacgcacg gcttcttccg gtgtctccga aaaacccact tcttcacaaa
    37681 gaacactggc atttatactg gtcagcactg ttacggccag gattcgtgtc gccgtacctt
    37741 caactgctgt acgtgctgct tcgatcatgg cacaaccgcc cgaagcatgg acagtgatta
    37801 atcctgcgcc caggtcggca gctgcccgtg cggcgtgacc gaccgtattc ggaatatcat
    37861 gatatttcag atcaagaaat acatttttcc cgagctgctg aacttctttg actatatccg
    37921 gtccaagccg ggtaaagagt tgtgcgccaa ttttaaacca tttgcagtca ccgcaggcat
    37981 gcacagcaga aagtgcttca gcgcggctgt ccatatcaag aaccacaatc agttctgttg
    38041 ccatttattt ttcctcacag aattagatat tcttttaaaa ctctggaaaa tatcaaactc
    38101 cgggacgatc ccatcccgga gttcatatag acgaatcata tcaagggcag ggcaggggag
    38161 tgcaattttt atttgaacct ggttttatat cacgaaagca ggttcgccac cttcatcatg
    38221 aaatcgccca gctttcctcc atattcagga gaaccgtcac tcaaaacaag gccctgccca
    38281 atagcgctta ctgtgtctat cgtatttgcc agaacaggaa gcgcagcatc gacgctttta
    38341 aagctcatgt gcacaaaagg tttgcgccct gtatgaacgc cacgacccgc tttggtcttg
    38401 cctgctttca ccttcagata acagacgata tctgtaccat ccacactcca ttgataaata
    38461 cgttcaggtt gtttcgctgt ccaagcctgc atgtccgtat caccggcttt gttcatctgg
    38521 ctgagcgcag tggaaaccat gtacaaagtc atctttacct taagatgcgc ctgttccgca
    38581 tttttgggct ttgctgcagg catcagcaat ttgagtccga atagcacttt aaaaacgcgt
    38641 atcacaagcc ccggcttttt catcagagca ggaagcagcg cgcccagacg cggcagaaca
    38701 ggcttgcccc tgaacatatc attcatggct tttaatgagg aaaacgaaaa gagcacatcc
    38761 ggattttccg taaatccctg ggtgacttca aatttcccgc gatcaaaaag aagccaggct
    38821 ccggtcttgc cgtccggatc gtccgcctca aactgcaccg ttcctgtaac atcggcaaac
    38881 cggtctttca tccgttcatc atcttcaagc atcacccgga ttacgggaag aaccgcattt
    38941 aaaacaagtc ggccggtcag ctgttctcga gtaagtgtca tgaattacac ctcatcttcc
    39001 cagtcttcat cttcttcaaa atcacgctgc atcagttcac cgactgtttc aataataccg
    39061 tccgtatcaa ttttatacat atgcatcaga tcttcaggat tgccgtgggg gcagaaggta
    39121 tcaggcacac ccagtttgtt aaaagcgcag ccttttccgc tggcagcaat cacgtccgcc
    39181 acagcgcttc ccaaccctcc aatcacactg tgatcttcca cggtgagcaa gcgcctggtc
    39241 tctactagcg ctttaagaac agcatcctga tctatcggtt tcagggtgtg catattcaat
    39301 actcttacac tgagtccgct gtttttattc atgatatcgg ccgcctgaac agcgtgaaat
    39361 acagtatttc cggtagcgat aatagtgata tccgttccgg atcgcaattc atgcgctttc
    39421 ccgatttcaa aaccgtactc ttctgattcg tagatgcgtg gttcaaaacc acgaccgatt
    39481 cgaatataca ccggtccctg aatgtccatg cattttttta ctgcaagtgc tgtttctatc
    39541 gtatccgctg gagcaaccac ggtcaggttc gggaaggcgc ggataatgga taaatcctcg
    39601 ttggcatgat gcgtggagcc tcccgagccg aaagacatgc cgccatgggt agctataatt
    39661 tttacgttca ggttctggta acagatgtct gtgcgtacaa attcacaggc acgcatggac
    39721 gcgaaagcgg acatggtgga cacaaaagga ataagcccgg ctttggcaag cccggccgct
    39781 gctccgaaca tattttgttc ggcaattccg aaattaaaaa aacggtcggg ataggcatca
    39841 ccgaatgtac cgattttggt ggatttggca agatccgccg acaggccgac gatcttcgga
    39901 tgctgcctgc ccagatctgt aagcaccagc ccgtagattt cccgctgcgt cattttatcc
    39961 gcatcataga ttgtccaagt cagagtgctg gctgtttcca tgcttttatc ctttattttc
    40021 tatcgatgct ttggctttgg cgatctgttc tgcatttaat ccgccatagt gccactccgg
    40081 tttcccttcc ataaattcca cgcctttgcc tttgatcgta tcgcagacca gcatgacagg
    40141 ggaacccttg tgcgcaatgg caaattcaaa cgcttccgcc agtgccggca aatcatgacc
    40201 attcacttcc cgggtttcaa aaccgaaagc acgccattta tccggaagcg gctccaggct
    40261 cattacttct tcggtaggcc cgtcaatcat caggtgattt ctatcctgaa taacaatcag
    40321 gttatccagt ttgtaatggg cggctgccat ggcggcttcc cagatggaac cttcgttgct
    40381 ctctccgtcg ccgatcatgc agaagacacg ccactctttc ttcagcacgc gagcgcccaa
    40441 agccatacca acagcctgtg acaaaccatg ccccatggag ccggtggaag catcgcagcc
    40501 gggcacttta ttgctgtcca gatgcatgcc cagcattgat cctgtttcgt tgaactcgtg
    40561 cagcgctgct ttatcaaaat atccgcgctc ggcaaggaca acagcatgcc ctacgccgcc
    40621 atgccctttg ctcagcacaa aacgatcccg atcttcccag gcagggttgg caggatctat
    40681 gcgaagatac ttccagtaca acagcgttag aatatccacc atacttaagg acccgccaat
    40741 atgcgcacca ccggaccatc cggtcacatc ggcaatctgg cagcgaatcg atgctgcacg
    40801 ctctttcagc gtggctaatt cctgttccgt caaggacata atcatttcct tttcgttaat
    40861 ttaatgacca aactcgcttt ttatcacttt aaaggcatcg tcagctattt gaagtgtttt
    40921 ttctatatcc ccgtccgtgt gcgcggtgga taaaaaccag ttgtgatgcg aggtaaagaa
    40981 tgctccccga cgggtacatt ctccacacca ggcctgctgc agcatcagtg tgtcatcatc
    41041 cgcgagacga agataaggca tggcgggttc gccggatacg accagagtgt agccatggcc
    41101 ggctgccaca tcacgaagcc cggaggtaag cttttcgcca agcacccgca tccgctccgg
    41161 tcccttttct gccttgaggg tagtaaggca ggctatagcg gcggcaaaag acccggcaca
    41221 ataccagaaa ctgcccgtat ggaacacttt tgcagcatca agacggagcg catctgtgcc
    41281 caccagtgcg gacaacgcat agccattggc gatagctttg gaaaaagcaa tcatatccgg
    41341 cttaaaacca tagtgctcat tggagccgcc caaatgcagc ctgaagccgg cgcgaacgtc
    41401 atcaacaatg acaacactgc cgtttttgtg cagcttatct atcacagcct gccaatagcc
    41461 ttcggcaggc aggacgttgt cttcaaaaat gggatgatga taggggcttg ccataaaagc
    41521 agcaacctga gatccatgct cggcgagcac ctggtccaga gcattcagat cattccaggg
    41581 aatgcggatt acatgctggt ggtcatcggc aatggtgcca tggtgcccgg gcgcctgcat
    41641 ccagggactt gtaccgtgat agcctccctt aatggcaatg actttatcac ggcctgtagc
    41701 tgctcttgcc accataacag ccaggttggt gacatcagct ccatttcggg caaaaaaagc
    41761 ccactcggcg acgggaacaa gatcgcagag aagttcagca agttccacca tccgcggaga
    41821 cgcaacgctg tttgcatcag aaagactgtg ctgctcctga aaggcggctt ccacggcagg
    41881 gtgattgtag cctagaacca tgggaccgta ggcacacata tagtcaataa attcgttgcc
    41941 gtccacatcc cagaaacggg cggcatcagc cttattggaa aaaaaagggt aggcttccac
    42001 aggagcatag ggagcagggc tgaaatgacc gtaaacgccg catgggatta cttttacagc
    42061 tctatcaaac cacttatttg attcttcgta acggtaaatg ttcactgtct tgtctcctgg
    42121 ggcgaactac tgcaaatatt ttggaatttg atcaagaatg acgtttgtat gatcgatcag
    42181 aggaattttg ccccttaatt gaatatcgcc tttggcaacg gcgccgaaac tgtccagcgt
    42241 gccgtcaaca agtaactggg caatttcggg gtttcgaaaa gaaaggaggg catccggatg
    42301 ggcagctttg cctgtaaacg cttttgctcc gcccttaccg tcataaatca catgagcata
    42361 aggcccttcg ggcaataccg acaactgcag ggtgccggca gacagttgcg gggcaatgcg
    42421 ccgggaaaca ggatccaact gaagcaattc cggtatggca aataccgctg tacttagtgc
    42481 cagggataaa ttgatcattt taaagcggtc atcctgaact acatcgctgg ggggcttcag
    42541 gaaatattca agcctgctgg taagcttcga aaattcagtt tttaaaaagc ccagcctggt
    42601 aaagccggag agaggcaggg gagaggcgtt attctccatc atattgttca aatgagcagc
    42661 agagcagaaa aagaggctaa ccgttccttt ttctattgta tcggctaaaa aagtgcaacg
    42721 tccgttatga aaatttaaaa ggatattggt atcgcgccaa atagataaac ggatggaaat
    42781 ttcccagtct ttgatcagct cccgactcag gtcgtctatt tccggaagca gctccacgtt
    42841 tcttaatact gctttaagat tgagtcgcgc tttcagctga tcaatcattc tcatactcct
    42901 tcctgaaaac catctatctt tgtgtcggtt gagagattca agagtaccat aatcacatac
    42961 aaagtgcaac ttatgaacgc cgtttggtgc ggtgctgctt cttctttaca cgggcagcta
    43021 tttcccttag cgactttggg aagtaccctt tctgctgata tactacggaa cagaatccca
    43081 ctattcttta agaggctttt ttccgggcag gaaaggaaaa ttgaaatggc ggaattgctc
    43141 atcggactgg acgttggcac aagtggtgtc aaggcattgc ttatggacac ctgcggaaat
    43201 gtcgttgcat ctgctttgcg tgaatacccg ctttccacac caaagcccaa ctgggcggag
    43261 cagaatccat cggactgggc ggaagcatcg cttagcgctc ttcaggagct ggctgcgtgc
    43321 gttccttcag gttcagaagt aaaagccatt gggctaaccg gtcaaatgca cggaagtgta
    43381 tttctggata aacataacga agtgctgcgg cctgctttgc tctggtgcga tcagcgcaca
    43441 gccgctcaat gccaggaaat taccgaccgg gccggaggag aggcggcact tgtaaatatg
    43501 gtatccaatc cggcgttgac agggtttaca gccccgaaaa ttctttggct ccgtgaccat
    43561 gagccgcatc atttcgagaa gctttgcaaa gttcttcttc cgaaagacta tatccgttgg
    43621 gtactcacag gtgagtttgc taccgacgtt tccgacgctt cggggacgct cctttttgat
    43681 gtacgaaacc gctgctggca taaagaatta ctctctttac tggaactcga ttcatctctt
    43741 cttcctgagt cttttgaggg tcctgaagtg acggggactt tatcgtcaca ggcagcacag
    43801 cgcacagggc tttcaaaggg tattccggtg gttgccggcg ggggagacca ggctgccggc
    43861 ggggttggct gcggcattgt caaagccggg acactttctt ctgcattagg taccagcggt
    43921 gtggtttttg ctttttctga agaaatatgt gttgatcctc aaggccgtat ccacaccttc
    43981 tgccacgcag tgccgggcaa atggcatgtt atgggcgtta tgttaagcgc gggcggtgcc
    44041 ctccaatggt tcagaaatca gctgtgtcat gaggaaattc aaaaagcccg ggaattggat
    44101 atcgacccct atacccttat cactgaagca gctgcaacta tatccccggg cgccgaaggc
    44161 ttattatttc tcccgtacct ttccggggag cgcacccctc ataaagaccc atatgctaaa
    44221 ggtacttttg cagggctatc gctgcgccat acaaaggcgc atatggcacg ggcaattctg
    44281 gaaggggtcg cctacggaat gcgggaaagt ttggatatca tgcgcgaaat tggcgttccg
    44341 ctctcagtct ctcgcatttc tggtggaggc gctcgcagcg ctctatggcg gcagattctg
    44401 gcggacacgg gtcgtgtagc aatcgctcgc ctggaggtgg atcaggggcc gtcctacggt
    44461 gctgccattc ttgccggggt aggggcgagg cttttcacgt ctgtggaaga agcgtgtgat
    44521 ctctgtattc atgagaaaga catacagatg ccggatccct ccgtagcaga tttttatgac
    44581 acctacgtgg cagaataccg cgcagtctat ctggcattga aaagtcactt caagcggatg
    44641 gcagcgctgg ttggctagta ttacgttgcc ttcgaagctt ctgttccgag accgcttccg
    44701 tcagaaacgc taaacgggcc ttgctgcatg gaagggttcc cattgccgca agaccagcga
    44761 tagcggcaga gggcatttca taggcatttt atctgagccc gtattttcgc ttctgcgcgc
    44821 tttttgtccg gttcctctta ctttcctatg gtttaaagcg cgcgcgcata gtgtctgata
    44881 atatatatta tgttcatttt tcgacgatca aaaaaatagc ccgcatgagg aatacctcca
    44941 aactccttat aaaaaagact tgccttatat gtttactata gatatgactt attttcgtac
    45001 acgtttcatt atgatacaca caattactga tttaaaataa cacgccattt caataagatg
    45061 ccttgctttt taaatgcatt tacagaccta atatttcata tcatatcagc acgatatcaa
    45121 actgttatta caaggatttt atgagttaat ctatctatag aaatatttgt ctcctctgat
    45181 tttttcaggt atccaaaatg cgcgtatcat attagatcag tctgaaatta ataggaaatc
    45241 atgacaaaaa aaatattgtc cgaagcagaa aataaaataa tactcttatg tgtattataa
    45301 taaagcagat tgactttgcg ttatacttgt gctctacaat gaaacaacga gacacccctc
    45361 ccctaatgaa ggatcacaag actattcgct ctgttcccgg gctatgacat ggttacagca
    45421 cctgagctga tcagagaagc aaacttcgga ctgcttcctt aattacccca ccgggcgttg
    45481 tgcgcttgac gggcaagcca tttggtttgc tctgcaagcg tcgccagcca ttggaccggt
    45541 gtcgctcccg ccacctttcc gtctgcagcc ggatgctgga aagccacagt tgtaatatgc
    45601 ttaatacctg ccgcataaaa gagcgccacc cgttcccccc aggaaaggct tctgtgttgc
    45661 tcatacgcag ccaaataaat atcggttctg ttttccaatt tttgtgcatc cacggaggct
    45721 cgcaaacata ggtttgaaag gccgtaggac agatcttcca gataacagcc gtcccctgca
    45781 tattctaaat caataatgcc ctgtatctcc tcatttacaa agagaaggtt gccaccgtgc
    45841 cagtccccat ggataatgcc cgtctcaaaa gtatcccgtt tttcttgtgc cagctcttct
    45901 gtggcttccc ccaaaaaggc gacaatggtg tcacagaaac catgcatctg ggcatcgttt
    45961 ctttcctgca gaactctctg ataaaaatcc tgaaatatgg cacgcggcac ttcgctgaaa
    46021 cgccacttac gggcatctct tggcggtgac ggcagtcccc ggcatacact gtggaatttt
    46081 cccaaacaac tgccagccac tttgagcgtt tgcacagtta acggtatggg cccgcccgat
    46141 aaaaaggact gcagttcaac tgcccagtta tctttggcaa taaagctttt gcctgcgtgg
    46201 gttcgtaaaa tctgcgccac gggtaaatca tggctgtaca aaaagtttga aagattgtgc
    46261 tgaaaataaa gggagtccag agttacggca tcattcgtat aggttttcgc caggtacttc
    46321 cctttttccg actccacaac aattttctta tgccgccgct ggtgcgtatt ttccaaatag
    46381 cgcaagggac gtatcgcgcc taaatgatag gcggcaggaa tctccgtgcc tagaatggtc
    46441 agtaaatcat ctcgttgatc cacgcctgaa catctgcctt atttcattac ataattatct
    46501 ttagacggct ggtacgcttt gtgactttcg ctgccggtct gtcgtaaaaa gagtcgcatc
    46561 tccgacaaag cgcagcaaat cttgtatata gcgtatatga ataagcgaca tttcagcaac
    46621 tttccagcac tttggacgaa ggaggctttt aattacatcg ggcacacgca aaaaagcctg
    46681 tgtaaagaag atagcctcct gtatatccga tttttttcct ggcgatacca ggaggacagc
    46741 actttcctcc tccttattct gtcgccaatt ttctaagacg cgccagtaat ttatcgtctg
    46801 caatgatttt ctgctatatt agaggcattc atacgttggc acaaagtcta cctgaagcct
    46861 ttcagttgcc gccttttaaa actatcgcct gattgtactc agacggagag aagtaattga
    46921 gctcccttac cgttttgacg ctggttcccg gcgaatgtga gcgtctgccc aaagcactgg
    46981 agagtgtatc ctgggcggat gtactttttt gtgtaatcga ttcccgtgcg cctcaggaaa
    47041 ccatcgatct tgtcagaaaa tataccgaca cagcaccggt tcatgaatat gtaaacgccg
    47101 ctgcccagcg caattgggct ctcccgcaaa ttacaaccga gtggactctt gtgcttgatg
    47161 ccgatgagtg ggtatctgac gcactggcgg aacgcattca ggcgatcata aaaaatccgg
    47221 acagtgccga tggatacaac attctgcgcc actcctattt tctcggcaaa ttaatccgtt
    47281 actgtggttg gcatcgtgac tacaacctca ggctttttcg gacagctaaa gggcattatc
    47341 tggacaaacg ggttcattcc aaagttatcg ttgagggccg tgtcgaaaag atacgcgacg
    47401 tcatgtatca caatacttac cgaagttttg aggagtattt ttccactttt caacgcttta
    47461 cgacgtgggg cgctcaggat gcctatgaac ggggaaagcg tgcagggcta agggacataa
    47521 ttcttcggcc cgttcaccgt tttttaaaga tgtacttttg gaaccggggc tttctcgacg
    47581 gatatcacgg cgctgttctc tgcggtcttt cagccttctc tgtcttcacc aaatatgcta
    47641 aactatggaa aatccagacg gacgctcaaa agccggaaaa ctgattccag ggcacttcgc
    47701 accatgacac agactctctc tcctatgcat gacgatctcc cggacaaggt ctacagcgtc
    47761 agcgaaacgg cgcaactggt aggaatctcc cctcatttgc tgcgtcagtg ggagaaacga
    47821 tttcccacag aagtaaaacc ccgtagaaac agcgcaaacc atcgcatgta ctcacgcgag
    47881 gatataatca gtctgatgcg aattaaaaca ctcctttatc acaatggaat gacagctaaa
    47941 ggggcgcgtc agcaattacg tcaagaaaga tttacggacg gacaaccccg tgatatcagt
    48001 ggtatggcaa gaataatcga tgccattgcc gatgaatcca gagccattct caacctgttt
    48061 gacccgaaag tggacgaact gattccggat caggacgatg ctttccacta acgcgtcaat
    48121 tgtctgtcag agcaccgacc ggggctgacc atataaacgt aaagcgatac tgcaacaata
    48181 agtgaaagaa aactaccgat ctgcagaaat aaggcgccac aagccttgac ataacactaa
    48241 agaaggatat ctaaactatg ggcggttttt ttgccgtaac ctccgaaaaa gactgcattt
    48301 ttgatctttt ttacggaacg gattaccatt cgcatctggg tacctgtcgt ggcggcttgg
    48361 cggtgcttga cggaaccgac ttccgcaggc acatccatga catttccaat gcccccttcc
    48421 gcaccaaatt cgaaaaagac atcctggcca tgcgaggcaa ttccggtatc ggtgtcatca
    48481 gtgatatgga gccccagccc ctcattatct catcgcatct gggccgctat gccattgcaa
    48541 cggtatccag ggtctccaat ctggattttc tggtacagga tgctttcaac cgccgggttg
    48601 cccatttttc agaaacaggc agcattggca tcaatcctac agaattgatc gcaacgctaa
    48661 tcaacgaaaa gacttccttt gaagcaggca tcagctatgc gcaggaacgg gtcgaaggtt
    48721 cctgttcaat tctgctgctc actgaaaaag gcatttatgt agcgcgggac cgtttcggac
    48781 gcacaccgat catcacagcg agcaaagaag gcgctgtggc cgccacgctg gagaatgccg
    48841 tttttccaaa tctggattat acaggacagt attatctcgg ccctggcgaa attctccgac
    48901 tgacgccgga aggtattgag caactccgca agcccgatgc ctacaagcaa gtgtgcgctt
    48961 ttttgtgggt ctattacggc tttcccgcct cctcctacga gaacattaac gttgaatcct
    49021 gccgctaccg ctgtggagca cgccttgccg aaaaagacac tgttgaggcg gatctcgtgg
    49081 cgggtattcc tgagtccggt accggatatg ccataggcta tgccaacgct aaaacagtgc
    49141 cgtacggcag acctttcacc aaatatacaa ctacttggcc cagaagtttc atgtcgccgg
    49201 gccagaccaa gcgtaaactt gttgcacgca tgaaattaat tcctgtgcct gatttggtgc
    49261 aggcaaaacg ccttcttttc tgtgatgatt ccattgtccg cggcgtacag ctcgccgaca
    49321 caataaagcg gctctatgag gcaggagccc gtgaagtgca tatgcgcccc gcctgccctc
    49381 ctctgctttt cgcctgtaaa tatcttaatt tttcccgttc caaatccgaa atggatctga
    49441 tcgcccgccg caccgtaaga gaactggaag gaggcgcaaa cggctccctt gaggaataca
    49501 cgaatccgga ctcagaaaaa tacaaggcaa tggttgaccg gatcaggcaa cgattgtgcc
    49561 tgactacttt gaaataccag cgtctggatg atctgatcgc cgctattggg ttgcccagag
    49621 aagatctttg cacctattgc tgggacggtt gtgaaggtca atgcggcagg caaatgacct
    49681 ttgccaatgg aatccaggaa accaaagact gagtccccta gaaaaccaac aggaactgcc
    49741 atgcctgaaa agcacgaaca caaacctctg cgctccacct tgccgggaat tgtcctcttc
    49801 cggttgattt tttctttcca ccttgccttt ttgcccgttt tcgccgccgg acctgagcgt
    49861 tgggattcct ttcgcgggcc ttatggaaat ggcattgtgg cgccgcccgg tgaaaacacc
    49921 ggcgttccgc taacctggag cgaaaatgaa aatattgcct ggaagacaga aattccctat
    49981 cagggctggt caacgccagt tatcggtggt ggaagaatct ggctgacaac tgcaactgtt
    50041 gacgggcatg atttctatgt cctcagcatc gatcaggaga acggacagat tcttctgaat
    50101 aagcatctct ttcattcgga caatccggaa gagctgggca atgaagtgaa ctcctatgct
    50161 tcaccgacag ccgctctgga agaaggacgc gtatatgtgc atttcggcag ttacggcaca
    50221 gcctgcctgg acacggctac ctatgaagtt ctgtggacac gaaccgatct cccctgccgc
    50281 catttcagag gaccaggttc atcggttatt ctttttgaag accttcttct gcttaccttc
    50341 gatggtgccg atgtgcagta tctggctgca ctgaataaaa acaccggaaa aactgtatgg
    50401 aaaacagacc gttccacgga atggccggat attgacgaaa atggggaagt gctccgggaa
    50461 ggtgactttc gaaaggcttt caccacccct tttcttgtga gagaaaaaga ccgcaccctt
    50521 cttttcagcc agggatctta tgcaacattt gcctatgatg cgcgtacagg caaagaatta
    50581 tggaaaaaga cgcacagtgc ctattcccct gccccctgcc ctgtatcggg aaacggattc
    50641 gtttatatac ccataggcag aggcgttcag gagttatggg caatgaaatt gagtggcaca
    50701 tcatcaattg tcgaaaaaga agcctcctgg ataattgccg gaaaaaaagt accggcggaa
    50761 ccatcccctc tcctcataaa tggtcttctc tatcttttaa gcaatgacgg gatcgtcacc
    50821 tgtctggacg gtaactccgg agaagagtac tggagcgaaa ggatcggcgg caactatatg
    50881 gcatctccta tttatgcaga cgggcgaatt tactgcgcag gcacgcgcag caaaatcacc
    50941 gtgctgcggg aaggcaaaca gtttgaaaaa ctggcggtca atgagctgga cgaaggcatg
    51001 cttgcctctc cggtcgttgc gggatctgcc atttatttgc gcacaaagac gcacctctac
    51061 cgtgttgaat tgaaaaaaga gcctggtgaa aacaactagc agcctcttaa actcttgtcc
    51121 aggatggaac gaatctgctt ttcccgtgcc tgaatgctct ccacgagctt aaactgcacc
    51181 cgtgcccgat tgagattctg cccgtcataa cgagtgttga agtacacatt accggctata
    51241 taatcggcaa aaaagcgcag ccccagttca aaactgataa ggcggatgct gtcatataaa
    51301 taatagatgt ctgcctctgt caaaaaagag cgggcattca ccatgtaccc cttgacgatg
    51361 gcttcgcacc agtctgtgtc aaagacaacc tgccccagat cgcgcacttc ttcaccagcc
    51421 ggattgcagc acgaacgcaa acaatctcca aagtcataat ggatcaatcc gggtttcact
    51481 gtatccagat caataatgca tgttcctttc cccgtagcat catcgatcat aatatttgaa
    51541 attttcggat cgccgtgtac gggacgcacc ttcaattcgc ccgcgtctct ggcatcctcc
    51601 aaaatagaac accagtcacg ccgtgcctca acaaaacgca tgcagcgctg tgcctcaggg
    51661 gagatcttca cccgttcctg cccttccgga cttgcaagag cctcatcata tttcttcaga
    51721 taaccgggtg tgatatgaaa gccgacaagc gtatcatgca gtttatctat ttcgatgtcg
    51781 ctgatcatac gctggaagtg cccgagaaca taccctgcct catgggcatg ctcaatattc
    51841 tgcacacgcc cataggagtg agccgatgca atcagcgtga tcgcacgcca gaattcgccg
    51901 tcatcatcca gcacgtaatc ttttccgtct tttgcgggaa tcacgcgcgg cagctgccag
    51961 atgcgatccg cagtatccat ttcctcttca atacgcctgt gaacatgttc agtcacaacg
    52021 cgcatattct gcataatctg ttccggatgg ggaaaaacgc gtttgttgat cttttgcagg
    52081 ataaaacgtt cctctgaaaa gactgtcctg aagattgcca ggtacgtgtc attcacgttc
    52141 ccttcacctg tatgttcaag agttacggca cgacctgcca catcaaattt ctgagctata
    52201 tggtgcgttt tcacaaatct gctttctttg gtgggttatt gttgaaccgg gctttttctg
    52261 aaaaagattt cttttgagta ttaaaccata attcagacat tctgtccaag aaaggtaaag
    52321 agcaccaagt ggacttatca gaaatattcc gcgcggcagc tgcctgtaat gtatttctgg
    52381 cttttttgaa ttcaattgtg atacgctgaa ttggcaagcg ttttttttgg cgatgagttt
    52441 cagcggaaag ggaaaagaaa tgtttggatt cagaaaaaaa tcgtcttcgt cggaaataat
    52501 cctttattcc taccctactt ttctttacgt ctggcctgta attgcgtttg gctacgtctt
    52561 tgccggactg caggccttag gatgggcatc acctacttca ctggcgtgga cctatatttt
    52621 tattgtggcg ctgaccatgc ttaccatggg tgttgacctc ggtcgtaaca ccagcatttt
    52681 ttgtctggtg gcgtttctgg cttgctggct cggtatttta tggcttcagg aagcaagaaa
    52741 cgttgaattt tttgcaaaac tcggtgcgat gatccgcagc cttcaacctc aaattaaccc
    52801 gcactttttg gtcaccttga gtacgctgct tctgattctt tatatcctca tgctgctctt
    52861 gtcgcgtgta aatgaccgct ggcgcataac tcataacgaa atagagcatc gctccttcgg
    52921 gcagaaagaa gatgccatcg gtcgcggcgc caagcgtacc cgatccgttt acccggatat
    52981 tctggaactg ctgatctgtc tgtcaggtac tatcgaaatt tacacggcaa acagcaacca
    53041 gaaactagcg tccattgaaa acattccttt tcttcccctc cgggcaaaaa aaatcagccg
    53101 tatccttgag atcaaatccg ttctggctac tgaagacgac gatgaagaag aggcttgagt
    53161 tcttttctgc tcttctcacc gattaacctc aatacatact acgccatcgg ttgtgtattt
    53221 gctttcgtaa agcagcaaac acaacaacac cttgtattcc tcctctgggt ttggtataat
    53281 ttcaaatctg atgaaaacac ctgaataatc tgttccggac agacagattt gtagaggagg
    53341 acgctgtgca gtttcgtccg ttcttgagca agaaaggggg aattgttgcc tgtctggtat
    53401 gggtatgcct gttgactcac ctggcttccg cacaggatcg gttgcggcag ccgttggaag
    53461 acggcgtttc tgctctgctc cagtatggtc agactataac actggaaatt acgctgtctt
    53521 cgtcttccca ggcgcaggcg atcatcagcc cttacctggc tgcccccagc ggatggcgca
    53581 gctttgtccg tggttcagca gcactgatcc ccttcgaacg tctttcggcg acgaagcaac
    53641 gccatgtgct gcttaccctt ttccctcttg attatgtgga tgccgaaggc tggcaccatc
    53701 atgtccgtat cgccagtgac aagtcaccgg aaacagttaa aattctttct tactggctca
    53761 cgggatctca ggacaatgcc gcacttattt tatccgttga tgccaatcga gcggtaaagg
    53821 aaccgcttca gcgcggacag atcattttat ttccccaacc ttttctgcgc ccagtcatga
    53881 aagaaccctg tgcttctttt gcctcacgaa acacggatca attcgcacaa ccgtttcaac
    53941 agcagacaag tccgaaaccc aaagagccgc catccgctac gtaccacgaa cgtcttcccc
    54001 taatgccgca ggcagcaccg cctcagacac ctgaaccgac tgcggaatca gccgaatcgg
    54061 cgatggctga aaactatcat aaagatctgg agtacggtga ggataaagaa gggcgctacg
    54121 ccatttatcg actgaaaccg ggagaaagtc tctattccgc agtcattgtt cgtttcaccg
    54181 actacacggc aaatgccgat gtcctgcagg cgtgcgaaat tgtggcaaaa cgaaccggca
    54241 tcaaaaatat gcaccatgtg actgtggaac aaaaagtaaa aatccctgcc gatcttttgt
    54301 ctgatcagta ccagccttta ggaacagtgc agcgtgaaaa ctatgaaaca atccgcgaag
    54361 aagcacggcg cctgcaggag attcgtaaag gaacgcgtaa tctccaaggg gttattcttg
    54421 ttctggatcc gggtcatgga ggcattgacc cgggtgcaat tcgtgaatcc gacggcttgt
    54481 acgaggacga gatcaattac gatatagcct gtcgcataaa agaaattctg gaaaaggcca
    54541 cacaagcccg cgtcttcatg actcttgaag atcccagcca gggatttaaa ccgactaata
    54601 acaagcggtt tgttcacgat aaagatgaac gcatcttaac gaccccacgt tacaccatcg
    54661 ccaataagga tatagctaat aatctacgct ggtatcttgc caacagtatc tattcgcaag
    54721 cccgaaagaa cggggcagat ccacaaaaaa tcgtttttgc cagcatccac tgcgattata
    54781 tctatgacga aacagtgcgg ggcaccatga tttatgtgcc cggagtcgcc catcgaaaaa
    54841 atgaagaaag ccatacttca ggcatttatg cacaatttga agaggtacgc caaaatccgg
    54901 ttgcttccag cacccactcc cagcgtgtgc aggatgaggc gctgtcccgc tctttatccg
    54961 cttccatcat ccgagccatg cgcacgcaca atccccctct gaaagtacac agcatcggca
    55021 atcctgtgcg cagcgaaatc cgcaagtccg gtggtaaatc ctacctgccg acagtgctcc
    55081 gcaacacagc tataccgaca aaagtgctgg ttgaagtagc gaatatgaac aacccgcagg
    55141 accgccagca cctggccaat cctgaatggc gccaatggtt tgcggaatct tttgtagatg
    55201 ccctcataag ccactataat aagtgatcct ctctgtatat cacgaatgat agcttccaag
    55261 ggttcacaac catgatactt ccagaccgtc cccgccggct gaggctcact tcaacgatac
    55321 gccggatgac aagagagacc cgaatctctg tggatcagtt gattgcgccg ctttttatcc
    55381 gtgaagagga gcaggtgcgg agccccatct cttccatgcc ggggcaatac caactgggaa
    55441 ttgccgacgc ggcggaagaa gcacagcgta tttatgacgt tggtattccg gcagtccttc
    55501 ttttcggaat cccggaacat aaggataacg aaggcagttc ggcatggcgc aaggatggcg
    55561 tcatccctcg agccgttgac agaattaaaa agcgctgtcc ggatctttgt gttatcacag
    55621 atgtatgcct gtgcgaatat accgatcatg gacactgcgg cattataaaa aagacacgcc
    55681 ggaacacact ggatgtggac aacgacgcca ccgttgaagt gctggttcgt gaggcactgg
    55741 cacatgcgga tgctggcgcc gacatcatcg ctccttctga catgatggac ggacgcatcg
    55801 gtgccatccg ctccgctctg gatgctcact cttttgaaga cgtcgccatc atggcataca
    55861 gcgctaaata cgcctcctct ttttacggac ccttcaggga cgcggctgaa tcgccacctg
    55921 ctttcggaga tcgacggtct tatcagatgg accctgccaa tgccctggaa gcacagcgcg
    55981 aaatttctat tgatattgcc gaaggtgccg atattattat gataaaaccg gcgcttccct
    56041 accttgatat catccggcag gcggcagaca atttcgatgt tcctgtggca gcatacaatg
    56101 tttccggtga atatgccatg gtaaaagcag cggcagcccg gggatggctt gatgaaaaac
    56161 aagccacact ggaaatactg acgggcatct gccgcgccgg tgcatctctt gttattactt
    56221 acttcgcaca ggatgcggca aaatggctgc agaaatgaaa gattcagcgc atgatacata
    56281 caaaatccaa acaactatgg gatgaggctc aacagatctt tcccggcggt gtcaacagcc
    56341 cggtacgctc ctttaaatct gtgggaggca gccccttctt cgtccacaaa ggacagggcg
    56401 ctctgctgca ggatgccgac ggaaatgagt atgttgatta cgttctttcc tggggcccgc
    56461 tcattttggg acatgcctgc ccggaagtag tgcaggctgt atgcgaggct gccgggcgcg
    56521 gcttcggctt cggcgcaccc acagaggtgg aaacgctcct ggcgcaaaaa atccggcacc
    56581 acttcccttc catgcagatg atgcgttttg tcagcagcgg cacagaagcg gcaatgagtg
    56641 ccattcgcct tgcccgtggc tataccggca gaaacattat catcaagatg gatgggtgct
    56701 atcatggaca cgccgacagt ctgctcgcaa aagcaggcag cggtatcgct acgttggctt
    56761 tgccggattg tcccggagtt cccgaaggca ttaccgaaaa caccattgtt gcgcctttta
    56821 ataatgctga aagcatcgca gagatcttca aagcgcaccc gggcgaaatt gcggcagtga
    56881 ttctggaacc cgtcgcaggc aatatggggg ttattccgcc cgctcccggt tatctcagtg
    56941 cgctgcgcag cctcactgaa aaagaaggtg ccctccttat ttttgacgaa gttatgaccg
    57001 gatttcgcgt agctatggaa ggctgccagg gactttttaa tgtcacaccc gacctgacta
    57061 ttctgggaaa agttatcgga ggcgggctcc ccgtgggagc ctatggaggg cgtcgtgaca
    57121 tcatgatgca tttagcaccg cccgggcctg tctatcaggc agggacgctt tcgggcaacc
    57181 ccgttgctat ggctgccgga ttaaaaacgc ttgaaattct gggtaaaccg ggactgttcg
    57241 gcgcattaac tgaaacaatg caaaagctct gtaaagaact gggagctctt tttgcccgac
    57301 acggcatccc tgtatatcaa acacaagcgg gcacaatggc gtgcactttt ttctcagacc
    57361 agaaagtgaa cgactacaaa gaagcccaaa aatcagatac cgccctctat gcacgctggt
    57421 ttcatgccat gatgcaggaa ggtgtctatt gggcgccgtc acagtttgaa gcagccttta
    57481 tcagcagtgc ccatgatgcc gtcgccatag agcgaacgct tgacgcagcg gaaaaagtac
    57541 ttgcacgggg tcttcccggc tgagccttta aaaaacaaac gaatcgattc aaggagtatc
    57601 ctgcatgtta actttctcgg ctttattgct tatgaccacg ctccatctgg ctcaagtaca
    57661 accggctgtt tctttcgaaa cagcgctgcc tgtctggatt gagggacggg aagaagaaat
    57721 gaatttgtgt gcaggattcc gctgcgcatt caattttccc ggcagtgaac cggtacatgc
    57781 gaaagtcacg gcttctactt tgtatcgcct ttatgtcaac ggcgcatttg ccggatacgg
    57841 acctgccaga ggtccccgcg gatttttccg cgttgatgaa tgggatatct caagccacct
    57901 tcgtgcaggc acaaatgttg tggctgtgga agtcgccggg tacaatgcaa acagttatta
    57961 tgtgcttgat cagcccagtt ttttacaggc tgaaataggc acaaaaaaca aagttcttgc
    58021 cgcaaccggg gctgggccca atcaatttaa ggcacacatt atcacagaac ggctgcaaaa
    58081 agtgcagcgt tacagttttc agcgcccctt tatagaagtc tacgcactga aaccgggttt
    58141 tgacctatgg catacccgcg tcgatcaatc tttgccccgt gctttgctgt ctgtttgtga
    58201 acctaaaacg cttttgcccc gtggtgtccc ctatcccctt ttccctattc agccggcagt
    58261 aaaaataatt tccgaaggga ctatgcggtc gggtgaaaac gataacccct tcttagatcg
    58321 gagtttgatg cagataggtc cgaagctgaa aggttttgtg cgtgaagacc tggcagtgga
    58381 aatatccgat gaactttgcc gcttgcatcc ggatcactgt gaagctgtgg cagaagaaaa
    58441 tgccaaaccc ggcaatttca ctttgaagcc caatgaattc aaagtgcttg atctcggctg
    58501 caatctgagc ggttttgtgt ttcttaaagt aaactgtact gaggcaacgc agctctatat
    58561 aacctttgat gagcagctcg acggactgga tgtcaattgg cgacggctgg gatgcgtcaa
    58621 tgcactcacc tggaatcttg ccccgggaac ctacacgctg gaagcttttg aagcccatac
    58681 cctgcgctac ttgaaagttt ttgcggcaaa aggcaatgtg acaatcagcc atgccgggat
    58741 gcggctgtat gaaaatccgg aagccggtaa agcgcaattt acctgttcgg atgaacgcct
    58801 gaatcgaatt tttgaagcgg gacgtaccac cttcgcccag aatgcagtag acatctttat
    58861 ggactgtcct catcgtgaac gggctggctg gctgtgtgat tcctatttta cggcgcgcag
    58921 cgctgaagta ctttgcggca actattcagt ggagcgctgc tttctcgaga atttcctgtt
    58981 ggcggaaagt ttcccatgcc tgccggacgg catgcttccc atgtgctacc ccgctgatca
    59041 ctatgatgaa gtcttcattc ccaactgggc aatgtggttt gttatggaac tgggcgagtt
    59101 tgctgatcgt attaacgacc ccgccttgat tgcacgcttt aagccccgtg ttgaagcgct
    59161 ttttaattac tttgagaaat tcgaaaatgc ggacgggctt ctggaaaaac tggaaagctg
    59221 ggtctttgta gaatggagcg ctgcaaacaa atttgtccag gatgtgaact atccgtcaaa
    59281 tatgctgtac tccggcgcac tggaaacagc agcacgcctc tttgatgtcc ctgcatgggc
    59341 agaaaaatcg gacaagatca aggaaaggat ccgggaacag gcaggaaaag agctgttttt
    59401 tgtagataat gcagtgcgcg acgccgacgg cgcattggtc attacagaca accgaagcga
    59461 agtgtgccaa tatttcgctt tttattttaa tattgccacc cctgaaaaga acccggaact
    59521 ttggcaaatt ctgcacagtg aattcggagc ggatcgtatc gaaaaaggat tatatcccga
    59581 aatacatcag gcgaatgcct tcattggcaa tatgctgcgc ttcgaacttc tttcccgggc
    59641 aggtctggcg gatcaactgg tcaaagaaac tgctgattac cttatgtata tggtagaacg
    59701 aactggatcc ctgtgggaaa atattcaaga tgccgccagc cttaaccatg gcttcgcatc
    59761 acatattacc gttaccttgt tccgggacat cctcggtgta tggaagatta ctcccaaaaa
    59821 taaaacagtg actgtaaaga tcaaccctgt ttctctggat tactgctccg gtattattcc
    59881 tgtgggagat gactttgtcg aagcatcctg gaaaaaagat ggcgatagca tacaattacg
    59941 actttcactt ccgcaaggct acaccgcaga ggtggaaaac aactccggat taactttgcg
    60001 tatggataat cccgctacga aagaaagttg acgctattta gaacacctat ccaacccggg
    60061 cctgattcca gacgtgaatc gggcctcttt ttatattatg cgcatttcac cgtatttttt
    60121 taaggataac ttcgtatttt ccggtccgta tttttcataa aaaccttgca tggcaagcga
    60181 tatttctctt taaatttgtg tgtcattgtg atacaatcaa ctcatggctc aaattgcaca
    60241 tgcttcaaag agaagcagac ataacataca caaacatcga ttgctgattg cctgtcccga
    60301 tatcactttc ggaacgactc tggttaattt tttaaaagcc tttccgattg aagctgagca
    60361 gtgccacgat cgggcaggac tcctgcgtag tgtcagccgt cgacaagcgg atatcgtgct
    60421 gctggatcta tctctcaaca cggatgagac attgaatctg gtgtccttta tccgtcacca
    60481 ggcacccagc gcacgaattg tactcctgtt tgaaatggat cagctgcagc aggcattgga
    60541 cggtatccgt cacggcgcct acttctactt gcctaaaagt tgtcctttat ccgacatagc
    60601 gctgattatt ggcaaagctc ttgaaaatct cagcaccgaa attcatctgg attcctatga
    60661 acagaccgtg tttgaagagt ttataggccg tacggacgca ctgcgccgca ttattgagct
    60721 catcaaaaaa gtagcgccaa ccaatagcac tgttcttctt ctgggcgaaa gcggtaccgg
    60781 caaagaagtt gttgcccaaa ccattcatcg tttgagtaca cgtcgtgaaa tgccctttat
    60841 cgctgtgaac tgtgcggctt tgccggacac attgcttgag agtgaacttt tcggccatgt
    60901 caaaggcgca tttaccggag cgcatcagga taagcaggga ctttttgaag cagcagacgg
    60961 cggaacactg ttcctggatg aaatcggtga acttcctgcc attacacagg cgaagttgct
    61021 gcgggcgctt caaggcggag aaattcgaag agtcggggcg attgaatccc ggaaaatgga
    61081 tgtacgcatt attgcagcca ccaacagaga tttattggag gctgtagaag attttcgttt
    61141 tcgggaagac ctgtactacc gtctgaatgt gattcaaata cgcattcccc cgctccgaga
    61201 gcgtctggat gccctgccgg ctctggtgac tcatttcatc gccaaatgca accaaagtta
    61261 taacaaaagc gtgcgcggcg ttgaccaggc ggcggctgct tatctgcagc agtaccctta
    61321 tccgggcaat gtgcgggaac tggagagcat catcgcgcac gcagtaattg ttacagaaag
    61381 tgactttatc ggagcagcgg atctccctga atatgtgcga caaggaattt ctcacagacc
    61441 ggcgcttccc tatcacaagg aggacgccat tctttcactg actgaaatgg aagccaacca
    61501 tattcgcttt gttctggatg ccacaaactg gaatcagact catgcagcaa acaaactggg
    61561 catttcacgg tcaacactct ggcgcaaaat gcgtgaatac aatctgcagg cacctcctga
    61621 tagcgtttag atctttccgc acttgcacaa agcactgcgc gctttaatta tcatgatgct
    61681 ttcagatatc atttgatcat gatctaatcc aaccggggag ttacagccat gagcgaacag
    61741 caacacggcg accgaagacg acatccgcgc tgcagcaaaa gggttgcctt ctgctggtgt
    61801 gaatctgaac acgggacatc aaaccacgtg gacaacatca gcgaatccgg gattctttgt
    61861 cagaccggag ctcccatccc gcttatgaca aaaattcagg tattactcga tctgcccaag
    61921 ccctttgaac aacgaatcga agttgatggc gtggtagtac gctgtgaagc cgatcccttc
    61981 aaagacaatt gttttaaagt tgccatattc ttttccggca ttaaagatgc cgatcgggat
    62041 acaatccgcc gctttgtcgc atccagcgag aaaacagaag agtaaaccct gatgctgttt
    62101 tggcagcgcc atttctgagg agaatagact tcatggacaa gaacacactt acacgccgcg
    62161 cattccttgc ctctgctaca gccggatcag ttattttaag cggacttcct gtccgcgcca
    62221 atacagcacg ggtagttcca cgtaaaatct cgcccaatga aaaaatcaat gtcgccggca
    62281 tcggtgtggg cggaaaaggg atgggtgata tcatgtcctg ccgccgcgaa aacgtggttg
    62341 ctctatgcga tcctgataaa aaaagagctg cagaggcttt ctcccggcta tccaaagcaa
    62401 agcaatatga tgattaccgg aaaatgtttg atgaaatagg cgatgaaatt gatgcatgca
    62461 ccatcagcac gcccgatcac agccatgcgc ctgccgccta tctggcaatg aatctgggaa
    62521 agcatgtcta tgtgcagaag cctctcaccc acacaattgc ggaagctgct ctgttaacac
    62581 gccttgccgc cgaaaaagac ctggtgaccc aaatgggaaa tcagggtcat tgcgaagatg
    62641 gtgtccgtga cctgtgcgaa atgctttggg ccggtgctat tggcgatgtg actgaggtgc
    62701 atatctggac taatcgcccg atctgggatc agggcatgga aaatccgctg ccggaagagc
    62761 ccattcctgc tgaaatgaac tgggatcttt ggattggtac ggccccgatg cgcccctaca
    62821 acaaagggta cgcgcctttt aactggcgtg gttggtggga ttttggctgc ggtgccattg
    62881 gtgatatggc gtgccacatc atggatcctg cattctgggc actgaaactt tatgaagccg
    62941 aagactttac ggtgcagacg gtgcgtcagg aaggtatgac aaaacagtca cccccgcgca
    63001 cgtctgtcat taaataccag tttcctcagc gtggtgctat gggaccggta gccgtatact
    63061 ggtatgacgg cggcgaaaag ccgacgtatc ctgagtcgct ccctcctggg caaaagctcg
    63121 gggacggaga taatggctct ctttttattg gaacagatgg tttccttaca gccggagagt
    63181 atggcggaag aagtcggctg cttccggaca cgagaatgca ggattacaag cgcccggatc
    63241 catcgattcc aagaattcct aaaggaaatc cctaccagaa ctggcttgac agcatccggg
    63301 aaggaaagaa atcagcatcg gacttttcct acgccggtcc tctgaccatt acagctaaca
    63361 tgggcaatgt ggctttgcag gcagcaggcg aagcgcttca ttttgatgcc cgggcactgc
    63421 gcatcaccaa caatgaacag gcgaatgcgc tattaaccaa agaataccgc aagggctggg
    63481 aacttccctg ctgaaaacct gaattaattt ttacttaccg gcgttcagtc tttcttaacg
    63541 ttgatgatta attccgcctt gctcccgccc acacgggaca tttcgcatta ttctttgcaa
    63601 ttccggggta ttgacttttt tcactaactg cgctataata ccccaaaata tggtgggaac
    63661 tgttgcagac ttcaaaggat ttcaccgaag gcacggggta tttagatcgg cgagtttgta
    63721 aaaaagtcat ttacgggaca gggaaaatac tgtcacgaat ccactttaaa atcttcaagg
    63781 agaccttttc atgtccatta aactggcaac cggtttgtgg gtatattcca gcatgcagga
    63841 tcgtttttgt ttggacggct accgcgaaac gccacggctg cccgacgtta tttcagccct
    63901 tgcgaagctc aaaggtctga aaggtgttga aatacagcaa accgatatta caccggaaat
    63961 gccggttaaa gaggtgaagc gtcttctgaa agacaacggt ctcgtctgca gcgctgtttc
    64021 cacaagtctt agccattccc gccgtttttc gcttgccggt tttgcgcagc agcaccagaa
    64081 aacacggaat gccgccattg atgaaggacg caaggcggtc gaaatagcac gcgctttggg
    64141 taccaccgag gtcgtgctcc gtctctttac agatggcttt gactatcctt tccatgtgga
    64201 ttacaccgct cattggaata cgattatttc ttccataaag accattgcca agttcgcctc
    64261 tcccgatgtg aatatttcgg ttgtgtacaa accgagggaa ccgcgcaaat atcttgcgat
    64321 ttcatcggtc ggcaaagccc tttcgctctg tcaggaaata gccatgaaaa acgtgggcgt
    64381 agctctgaat ttttctcatg cgctcatggc aggagaaaat ccggcggaaa gcattgcctt
    64441 cttaaatcga tcgagcaaac tgtttcaggt ttacatcagc gattgctatg ctccctggga
    64501 tgacctgatg attccgggca gcctccatct ctgggaaacg cttgaagcac ttttctatct
    64561 gaaaaatgcc aagcacaaag gctttgttac tattgacatc cagccgcagc gcatggatcc
    64621 caaccatgcg ctccagcttg ctctgggcaa tatcgccatc ctttggaaaa agctcgaacg
    64681 cctcgacgcc accgaactgc gcaaggccca acgcaccctc aatgctgtag aaagccagcg
    64741 catcatccgc cgcatccttt tacaaggctg acctttttcc ccggcatatc ggctttttta
    64801 aaactatccc gaaggaacga acacactgct caccggcaat ttgcaaggtt taaagtctgc
    64861 tcagatcaaa gcgttggagc gcctgtctac tcgttctatg cacgcacagg cagtgatcag
    64921 tccggaaatt gcgcgaaccc tgacggaatt atccttcgat atccggcggc agatcggatt
    64981 gattatagac cgacgcggca gaattaccgc cgttgtggta ggggatgccc gccgtctggt
    65041 gctgcctgat atccaccgtc gcggtatgaa ccggttaagc ggcgtgcgct tgatacatac
    65101 ccatctgacc aatgaagcac tgtcgaacga tgatcttacc gacctggtca cattgcgcct
    65161 cgacctgatc gctgctctta ttgtgcagac agatggaatg cccggatccc tttacagtgc
    65221 tcatctgctg cctgacaacg ctgcacaaga cccctggaac ctcctgcctc cctcttctat
    65281 ccatgcattg gaagaagact ttttagattt tatcgaggcg ttggaagagg agttctcgcg
    65341 aacacaatac cagctcagca cggatgaccc aagggaacgt gcgattttaa tccacgtgtc
    65401 gacattgcct cccagcattg cccatgattc tcttgatgag ctgaaagaac ttgcgcgaag
    65461 tgccgggata aaaacagttc aggaagtact ccagagacgc tcgatagaca gccgccttgt
    65521 cctgggtaaa gggaaaatgc agtccgtttt aatctcagcc atgcaaaaag gtgcagagct
    65581 gcttgtcttt gatacgaatc tttccccgtc tcaactccgt tctctgggcg attttaccga
    65641 tttaaaaatt cttgaccgca cccaactcat tctggatatt ttcgcacaac atgccgttac
    65701 ccgcgaagga aaactgcagg tggaactcgc ccagctgcga tacctgcttc ctttgctcaa
    65761 tattcggcaa aaggcactgt ccagactgac aggcggtatc ggaggccgtg gtccgggaga
    65821 aacacgtctg gagatcgaca ggcgacgggc gcgggaccgt attgcacgac tggaacgtga
    65881 agtggcacag cttggggagc gaaggcgcct ccgccggcag gtgcgcacag agaaaaatgt
    65941 tcccaccgtc gccgtagtcg gttataccaa tgccggaaag tcaactctcc tcaatcacct
    66001 tacaaacagc gatgtaaccg ccaaagactt cctttttgcg acgctcaacc cggtatccag
    66061 acgcttgcgt ttccctcagg aacgtgaaat aataatcacg gacactgtcg gatttatcag
    66121 ggagcttcct gaggatttga tggcagcttt tcgaactact tttgaggaac ttgacgaagc
    66181 cgatctgctt cttcacgtgg tggatgcgca cagcccggat ttatctgaca aaatgaaaac
    66241 tgttcttgat acagtccgga gtctggagct ggatcacaag ccgattgtta cggtgttcaa
    66301 taagatagat ttgtgctctc ctgaagagat agaaggactt ttacaccagt atgagggcgt
    66361 tcccgtttgc gcattggaca gagggagctt tgcaccgtta ttgacgcttc ttgaagaacg
    66421 cctgtggccg gcagattagg agaaattatg ctcagaattg ttgtcgcact cgttctttcc
    66481 gtcctttttc agacagcatc gcatgcgcag ccctggcaga atccgcctga ggctcccccc
    66541 ccttcacgcc aggacatgat aaatcgtttt cagcaactga ctgctgccgg cgctccattt
    66601 aacgtgtcag cggaactgta tgaagaatac ctcggattaa ttcagaacct cgattatcag
    66661 gatttatggg cggatttatt agagaaaaag ctcaatgaac cgggcaatca ggaagcgctt
    66721 gacgtaaaaa actggcttgc tctgggcgaa gcgcatttta aaacaggcat gcatggcacc
    66781 ttgaaggcag ccgaagcctt tcagcatgcc ctgacgctaa gtcccggcca tgccgatgca
    66841 ttagcacgcc tggggcagct gcaccaccgg gaaggtcaat acgatctcgc cgaaagtaaa
    66901 tacagaaaag cactggaatc agacccggaa aacgcgcttg cccttatagg cactgccgta
    66961 cttcaaataa gaaaaggcga catcctgaac acctcccgga cgctggatca gttgggtgtc
    67021 aaggcgcagc cttatgacgt agaaaccagg cttatgctca ggcaggcgct gactgctttt
    67081 gagcatcacg gaggctggtt tgatgatacg gcggaaaacc ataacgcata tgcaagactg
    67141 ctgtatcgct cagggcggct cactgatgcg attctggcgg cccgacgggc agtcacgctg
    67201 gaacccgcac ggagcgacat ctggaatttt attgcaagca tgcagctgca gatcggcaat
    67261 ctggaacagg caaagcaggc gtatgatcag tctctggaag cgaatcctga ccaacccgct
    67321 attactgata cacggcgcca gctcatcact accctgacaa cttcgggtcc gggcaaagca
    67381 ccctgacagt gccgtatcaa acgaaatcag agacattttt atttaaggat tgtccatgct
    67441 cagaaaaaaa agactggttc tctacctctt agttgcgggg ttgatctgga ttggatggcg
    67501 ctattcccta ccactggcag gcgtgctgtt gctcacagct ggtgtgctac tgggcctggt
    67561 gaaactatta cggattgctc aggcgcattt tggaatgaaa cgcttgaacg aaaaaacaaa
    67621 tcaacgctat gcggcgttga ttcaaaaact tccggggcag gaagagtctg agacctggtc
    67681 ttttgccgtg ctgggcgata cacgcaacaa cgtccagatc gcctcagccc tttataagaa
    67741 aatcgagtct tatgctcccg taatggcatt tcataccggc gatatcgttc gcggcggaac
    67801 tgcagacgaa ttactggatc ggcacattgc tgttgttgaa agagacatgc cctcaatccc
    67861 acttttctgc atacccggta atcatgaaag aggccccaaa agagactacg ccgcttttaa
    67921 acggctctac ggagatgacc gtttctcttt ttccttccgc aagtgtcttt ttgtcggctt
    67981 taacaacaat ctcaaggact atgtgcagga agaagacctg tcctttttgc aggaacaact
    68041 gcagggagag tacaggcacc gctttgtttt tctccacatt ccccccgcct tttttgaaga
    68101 aagttttgtg tcagacacaa ggcggcgcgg gttcaaaaag aatgccgaag tttttcatca
    68161 aatgatggtg gagcacagag tcgatgaggt ttttatggcg catatccacg gatatgcaac
    68221 acttgatcgg gacggcgtac ggtatactct gactgcggga ggcggtgctc ccctgagcca
    68281 tcgtattgct ccggaaaaca ggcactacca ttttatcgat tgtcaggtga cccccagcgg
    68341 cataacacgc acactgcacc tttatataga cggtcgctgg caggaacgag aaattcgtta
    68401 ggaaggcaag ggctcactgt tttattagca gcgccgccgg gaggatttcg agtattgcgg
    68461 gcaccccgcc cggtgactcc ccatctattt cgaacaatgc cgcatcctca ggctggcagg
    68521 gctctataga gaccttcttt cctctgagtg cgacaaccat gtcctttttt ttcagatgcg
    68581 ttcctttgta gatcagagga aaccacttca gcacctgaaa aagagacaat ttcctgatcg
    68641 caataatgtc aaagacaccg tcatccaaaa gggcttccgg tgcaatttgc ataccgccac
    68701 cgaaaaaacg cccattgcat actgcaatgg tgttggcttc cagcacctca tcataatgat
    68761 catctacacg caagcgcagc tttttgcttt tatagcgcaa aagcgttgaa ataacacccc
    68821 actgaaaggc aatttttccg cccagttttt tccagaaaac gaggctattc accgtgcggc
    68881 aggtcgctcc gctcaagccg cagcttgtcg agtttatgaa ataacggatt tctgtttcgc
    68941 ccgttctgtc attatggtaa tgcaccctgc cgacatcaat acggcgcgtc ttcccttccg
    69001 caatactgtt aattttttta tagaaatccc ggcttgaaga aaaacttcgg ttaaaatcgc
    69061 agcctgtgcc gctgcagata tgactcagca cagcagaggg gttaacgggc aggtcatttt
    69121 caaaaaaacc attaacaact tcattcaagg tgccgtcacc gcccacggcg ataatatgct
    69181 ccgccccgtt tttcagggca gcccttgcca gatatgaggc gtccagaggg gctttggtaa
    69241 aaacataatc aattttgcct atgtgccctt ccagtgtttt aaatattgag ggccaacgct
    69301 tcaatgtctt tccggtagac gaatgaggat taacgatcac acagactttt gcggcggaag
    69361 aggagcgaga agaaaggctg ctcaaaaatc cctgtccggc acagtcctcg ggatcatcct
    69421 gagaagagac tggcagagtc gccgtctcat aaacagccgc attcaaaaca gttcccgatt
    69481 cggcagtaca gaaccgcctt cgggaaaaga taaaagaatt tagcagtctg tcaagaatgc
    69541 cgcctgtttt atacatggtg cacttaccct gtaatacact ttttgtttat ttttgaagcg
    69601 gggaagaaaa cccactgcat gagccggaac accgtacgaa aagcatagca cggacagcgc
    69661 aaaagagaca aaccgccttt ttgcattccg gactgtattt catgggatcg ggtgtgctaa
    69721 ctttatcatg cgttgtttgt actttttcac cctgaataaa ggagcgtctc ccatgaaaca
    69781 ctgtatgtct gtactggttt tgcttgcttt tttctgtctg tatatgccct cctatgcgga
    69841 agaatcttcc tgttctctgc atccggactc ttgcgcctgg aagcctttat ttgctgatga
    69901 tttgtccaat accgtgaaac ccgatggcgt ctggacagta gaaaacggag aactgactgc
    69961 ctccaaggac gaagcaatct ggacggacag ggagtacgag aattttattc ttgacctgga
    70021 atttaaaaat gccgaggaaa caaacagcgg cgttatcatt tattgcagcg atctgaagaa
    70081 ttggattcct aattccatcg aaattcaaat agccgatgac ttcgctgaaa aatgggcgca
    70141 gtcgccgaaa agctggcaat gcggtgccat attcggccat ttacccgctt cgaaaagtgt
    70201 ggttaaaaaa cctggagagt ggaacagaat gaccctgcgc tgtgcaggca aaataatcga
    70261 agtcattctc aacaatgaaa aaataaacga aatcaatctg gatgactaca catctgccaa
    70321 agttaacccg gacggcacag agattccggc gtggctcagc aaacctaaaa gcgaattggc
    70381 aacgaaaggc agaataggat ttcagggcaa gcatggcaat gccccggtct ggttcagaaa
    70441 catcagaatt tttgaaacag caagcaactg aaatattgtt cagtttcttg tttttttaat
    70501 ttagaattat tcctttgaat atggtaaaat taaggtactc aaaagcctat tgagcaagac
    70561 agttaaatct tccctgtaaa ctcaggttta ccctgccatg tacgatacgg tgtaagagca
    70621 attgaccttt cgtatgaacc gggagtctaa tatttgagca gtctactgta tgcccccttg
    70681 tgcggaaacg ggatggctga cgtgaagaca cccccttatt tagggaaagg gttcattacg
    70741 cttttcacat accgaccggc atgtgcgggg ttttttttgc cacagaaccg cctttttgca
    70801 ctttttgtgc gcgcaatggc gtcactctac gcactctccg gcgcgccatc tcctctctat
    70861 tcgtgaaccg ccatctctgc acacctgtat gcatgtgaaa agcctcaaac ggatctgctt
    70921 ccatcattgc tcagtccttc tttattaaag attcaactgt tcataaaaaa aggccttctt
    70981 ctcttgtctt cgacagacgt tgccaaaaga agaaggccaa taacattcgg gcagatagaa
    71041 ttattcagac ggggcttctg atgtatcccc gattgcttcc ggagcctctt gcccgctaga
    71101 agctgaaggc agatctattg ccacaacaga ctcctcttta ggaatcatcc ctgaggcatt
    71161 ttcctcaacc acactgcctg ctccggccgt ttctgttgca gaggaagccg ctgcagcccc
    71221 ggaagtttct tctgcggcag caaatgcgtc tatgtctgca aaaagacgta ggtactcaag
    71281 cgatttagcc gtgaaaaggc agggcagcca agacaaagac gcctcatagt taccgtcttt
    71341 ttccgcaccg aaggacaaca ctttatgctc tccatctgcc agcacaaaag aaagcgtagc
    71401 cgcagcttcc acctctgatc cggcccggga aagtgtaaag ctgtccattt ggaaagcgtt
    71461 aaccgtcata agataatctt caactttatc ggcaccgggc gcaatcggct ttccgttgaa
    71521 tgtccctgcc cactgctcgg tttgcggatc ttttttcaat gcaagatttc gtttctcctg
    71581 ggtaacattc aattcgacca ggttttccga cggaatatca aggactgcca gtgagaagag
    71641 atctcgaaca ggcggtaaaa tgcgctctga atccgcccgt gatatgacca gaatcgaatc
    71701 agagtcatca aattttacat aacgcccgtc aacacttaaa gacggacttc ctgcccgcaa
    71761 aacacgttct gaaccgtcaa gcatcgctat acgtatggtt gtgtccaacg cacccgtatc
    71821 ggccgcctga tcggcatagt caatggcttt taacgatgcc aatgcggtgg caatgccttt
    71881 cagtgcagtg gtttgctgtt caagattcag ttgaggctca ataattttcc agtccgaacc
    71941 gtctttctcc agcacaacct tgccttcagg gcgctccatt gtaatctttg ccaggctttc
    72001 ctgttccaga ttccattccg gcaaagacac aattttgctg cccgcaacaa aaacctgttc
    72061 aaagttgtat ttggcaacct gatacatcaa ttcaggctcc tggccggcct gctgcaggta
    72121 atacttatct tctgcaacat ttcttccgcc gtaaaacact ttctccgatc catcagcaaa
    72181 agaaacagtg gcagtaaaag ccggaggatt gaagccgcaa gtgtcctttt cgtcgggtcc
    72241 cgccacatcc attgccgtga tcgtactgaa ttttgtcagg atggttttca cttctgtact
    72301 tttaaaatct ttggcgccgg gatcagttgc cagtgcccat tcgtactgtt tgtccggttg
    72361 ggcgggtacg gcgctctctt cgctgcccgc ttcctgggtt tctggctctt tttccaattc
    72421 tttacggtca aagaccaatt ccttgtccgg gtattttaga gtcaacttcg caacattctc
    72481 cgcatcgaca tcaaaaagct gcgttttcag ccatttattt gattttgggc gaagatcctc
    72541 ccctgtttcg ctcgcccctg cttcccggcg aagattgttg ttttcaacaa aaactttgga
    72601 ctccccggct ttacgcacaa aaacggttcg aaaatcagcc gcttttccga aaagcaattc
    72661 aagagcaggg ctttctccgg aagcaagcca ggcctgcaca tgaaacgctt catcgtcttt
    72721 caaagcgaaa ttggcaagtt tctcatcatt atccgctgtg gcgcgtggtt cacctttcat
    72781 cgcaaggatt ttgccgataa agtcatcaac agcattttta tctgcaggtg cattgtgcag
    72841 gctggtaatg agccactctt cccttttttt ctgaagaact actttgtctt cgggggcgga
    72901 acctgaaaag agttccactc tgtccagagt actttttgtc agggtatcgg gaaccagtgc
    72961 tgcaagcccg atctgctccg caatgggtgc cggttttttc tgattcgact tttgccacac
    73021 caccagcccc aacagaagca ccagaataac aaggaacggt aaaaatgatt tcattttcat
    73081 aagaaacttc ctatttgctg cagattcgct gccttattga ttgttggaca ttgccgtacg
    73141 actctgcgcc atggtatagg cgttccgtga acgacggcga agaacaaagg agctgatacc
    73201 aacggcggcg ataatcacat tcgccagacc gtagttagca agctgccaaa tcgtacgctg
    73261 gcgcccagta ggccgatcaa tcaggcggtc aataggtttg gtgccgcgca cattaaccag
    73321 attttcgctc aatgtcacag cgtcaacact gttcaaaaac agatccagat tggatgcctg
    73381 caaaaagttt ttcttgaaca tttctgaaca gccgaggaca atcagttttc cgggcgccgg
    73441 cgtaacagca gctgccgggg cttcctgtgc gtcgtcttca ggaacaggag gcataccggg
    73501 atgcggctgc ggtttcggcc aggccggacg ctcgctgtcc gcataagcat cggggaattg
    73561 tccttgcacc aaaaccataa gcggataagc ttttcctgaa gaaggcggct caaaggatac
    73621 ctgtgacatg ggggcatcgg cggcaacaga ccaggcgttg gcgcttgttg acatgagcac
    73681 ttcagcatca agttttaact ctttcagttt ggcttcatcc atggagaccg ctgttcccca
    73741 gagataaaga atggcggcga ggcggtttgt aatggatttt gcctggttca tggatttatt
    73801 gttaatgaga atatgggtcg gtaaggtgaa gggctgtccc atcagtgcac cgaaaagccc
    73861 gccgccctgg ctctgcacag tcagcgtgac attgtttgta tccataagca cgtctttgct
    73921 gacacccagc ccgtatttct ccagcagctg gttcacttcc ggattttcat cgcgacgggt
    73981 aattctgttt ccgtcccgtg tcgcctgata gtcccattca tattgctgag cggcgataat
    74041 cactgatttt ccggaataga cggcacggct gatttcccat cgctgccggt cattgagaga
    74101 tcgcgggtta acaacgacga gcgtatcgta tttttccggg agcggatctt cctttgtcag
    74161 ctcaatacgc tgaacatcat atttttccat ttcaagaata cgctcaaggt aaacataagg
    74221 atcttctgtt tccgggacgg gctgccccat ctgcatgagc atctgtcgca tttgcggatc
    74281 aatgttaacc gcttctttcg gagcaaccag agccacgaca ggtttttctt cacgggtcag
    74341 tttgtagata gtattagcaa gctgatactc aagatccggc aaagtattgg gcataatttg
    74401 aggaatgatt tcctccgctt ttgcttcata gcctactccg atactggaat ataccagctg
    74461 gctagtgacc tgatcctcgc tcatcgcctg gacattgaag ggttccacgc ccttgtcgag
    74521 catccgtttc tcaatcgcct cgtcttcagt tttatctttg tcgtctccaa cgttgaaatc
    74581 gcttccggta gtaatagcat tggcggcttc aagataaacg gtcgcatagt tcagcttccc
    74641 gccagatgcc agtcgcagct cttccagttt gtctgtaata tcgcgctcca tattccgcat
    74701 ggctgtgggc atcttatcgc gcggcgtaat gtataacttt atctcaattt cgcttgaagc
    74761 ttcctgcagg atcgctttgc ttgacggaga gatcgtgtag attttatcct cagtcatatc
    74821 aaagcgcttg atgctgcttc ctgccatgag gtagttaaaa agcatgccga taatcaggca
    74881 gaccgctgta atcataccga acatggttcg tgcgcgcggg cggtttctgc catcgataaa
    74941 aagaatgttt agtgtcagga aaatgacagt ccaggtcagg aaaaagagta catcagacag
    75001 gtctatcaca ccgcgcacca gcggctggta atgtgtaaag atgccaaaca ggtcagccaa
    75061 catcgagccc agcccgctta tccgactgtc tatataggaa gcaatgaagt cagtgccgag
    75121 gaggaaaaaa agaaagcatg cgagaagtgt cactacaaat gcgacaatct gatctttaaa
    75181 aaagccactg aaaagaatac ccagcgccag aaaagtggca cccagaagca aagtaccgaa
    75241 ataaccgctg accacaacac ctgtatcggg attacccaga gaaaacagca tcagtggcac
    75301 tgtgaatgtg gcaatcaggg taagggcata aaatgtgagt gcagcaagaa attttccaac
    75361 ggtcaattcc cacgcctgca tgggaaaggt cagcagcatt tcccaggtgt tttctttacg
    75421 ttcttccgcc caaatacgca tcgtgacagc aggaataaaa acgcacagaa gaagcggcag
    75481 gttctcaaaa tagggccgca tatccgctac aggaaagagg aaaaaggcgg tgacgtacaa
    75541 accgacactc atggtcacga agaccataat aaaaatatat ccgatgggcg aagtaaaata
    75601 agtgcccaga tcccgtctaa gtattgaaag agtattgctc attcttatta tctcctagct
    75661 ggcggtcttt tgcccggcgg ctttttcggt caatgtcaaa aatgtttctt ccagagaaag
    75721 gggtttgtcg gaaagctcac ggagtttcca cccctgttgc tgtaccagtg cattaatttc
    75781 ccgccagggg tttttgtcca ctgggctatg cagtacaaaa gagctgcagc ctcctgaaga
    75841 cccccggtgg accacctgct tcacaccatc tatgccgccc aataaacgct caaactgctc
    75901 gcgttcacct tccagactga tgaccagccg ttcagattgc ttcgcacgcc cccgtaattc
    75961 cgcaagcgtt ccgtctccca caatagttcc gcggttaata atgacaattc ggtctgctgt
    76021 tgcctctact tcctgcaaaa tatgcgtgga gaggatcacc gttttttctt cagccaattg
    76081 gcggatcagg ttccgaatct caagaatttg atggggatcc agccctgaag ttggttcatc
    76141 aagaataata atatcgggat tgtgtaccag cgcctgcgcc agagcggtgc gctgtttgta
    76201 ccctttggaa agctcgcgga tgactttccg gtacataggc cggagtccgc atgtgtcaac
    76261 aaccgcatca atccggtttt ttaaagcggc acctttgagt ccccgggcac gtgccacgaa
    76321 atccaggtaa ctccgcactt ccatttccat atacagagga aggatttcag ggagatagcc
    76381 gatcgatttt cgtacttcga gtgggttctc caatacgtct acgccattga cctcggctgt
    76441 cccgcttgtg ggatacagat aggtggtcaa tattttcata gtcgttgatt tgccggcacc
    76501 gttcggcccc agcagaccta caatttcgcc tcggttcact tcaaaggata cattatccaa
    76561 agcgaccaca ggcccgtaat gcattgttaa attctttgcc ttaatcatgt actaccttgt
    76621 ctccactgtg cagaaattca cggaggtgtt attcgaatca tccgcattta aacacggaca
    76681 caccgctacg cctcgataag agcaacaatc atgccatcgt tttattcgaa gagcgcatga
    76741 aattatatat acataagcac tttattatca ataatttatt gcaccgtaac cctctgctga
    76801 tcagaaacag ttgttctgca atgcccgatt tgagacatgc aatgccttaa aaggtttcgg
    76861 tttatttgag gttacagcgg tcctcgtcag cgaccagcgc atccaccccc tcgtcatatt
    76921 caagaggatg agctccctta tgatcccccg gctccatatg cgaagtgata taaacctgga
    76981 aatcaggaaa gagagagcgc atcgctgact cggcaactgt aatccgccgg tgagcttcct
    77041 ccacagaaag cgagcgatca accagggcat gaaactctat ccaaagtttg ttttctgcct
    77101 gacgatgacg cagctgatga taactcttaa tgcatcccga ctgaatctgc tcatccaggc
    77161 acttctgaag caacaccgta ttttcaggac atgcccggtc aagtaatccc tgcgatgctt
    77221 ttttcagcag cccataggcg gtaatactga tattgcatgc cataataaga gcgaccagcg
    77281 gatcaagcca cttccacccg gtaagccaga caagcccgac accaaggacg acaccgaaac
    77341 tcgtccacat atcagacaga acatttttgc cattagcaac aagtactatg gaatggtacc
    77401 gttttccaac aaagacgaga aaagagccga gaagcgcatt gataaccgcc agaacaaatg
    77461 tgattagcag accggcgccc agcggtccca gaacaacgcc atagaccagg gagtgacctg
    77521 caagataaaa gacactgagc gccgccgcgc cgataagtgc gccttcaaat ccggcggaga
    77581 aataagcgat cttgccatgc ccataggggt ggagcctgtc cggaagctgc gtggaatacc
    77641 agaggctgaa agcagcgaat ccggtcgcca gcagatgcac aacactctct gcggcatcgg
    77701 ataaaatcgc ataactgccc gtcagccagt atgccgccat cttgcccagc agcattagaa
    77761 cagccacgac aaggcttacc cccatggcaa cataccgcat gtccactgat ttcagtataa
    77821 ccataacaga cgcatcaatg ctgaggaaca gccatcgttt ttatacaagg gactctctta
    77881 agaccgcata cttcagccag ttaacaaact cacactcccg gatgtcatcc agaaccacat
    77941 cgtcctcaat tttcagctgc cagcgaatat ttttagtgtt gtccggctcc tgaaagacct
    78001 tctccaattt atcagcatag gcaataagat cttctctgtc gctaaaccag ccctgtttta
    78061 taagacgctc ttctcgggca tgaatcaggc acgccatctc tttcaggtcg tactccggat
    78121 gatactgggt tgcccagaaa acacctcttt tgtaagtcac tgcaacagcc tgcacatcac
    78181 accagtagtt acctgccaga atttctgcac attcaggaac ccgggcaaca taatcatcgt
    78241 ggctcacgaa atgcgaataa acagggggtt tccctttcat catggggtgt cgcatgccgg
    78301 cttcggtctg cctgatattc agtgcaatac ccatttccct gccacgggga tgcggctcgg
    78361 tcacgccacc cgctaccgtc gttgccagtt ggattgccca gcagctgccg aattgcggaa
    78421 ttccagcttc ataggcacgc tcacacaatt tgatatgcgc cttaactctg gggtcgtcat
    78481 gataaatagt taaattacag cctggccaga taaccccggc atatccttcc agttcctcgt
    78541 cggtgggtgc ttccaccccc gggtcactgc tgtaccagat atcgtattcc gcatcaggca
    78601 tacgtttcat taacaggctt gcatagagtt ttcctgccaa tgtcatgccg acccggtcaa
    78661 attcgtccct gctttctttc gaatacccat cgggaatcag gaagcgtttt ttggaagtca
    78721 taatcagatc ctttgctttc tttaatcttc tatttttcag actgccagac gaagttaagt
    78781 atacgactgt cttccggccc gcctggtgtt ttatatcatt tcgctaaaaa actgaaaggc
    78841 cttatcgcga aaccccggat aactttcatg cataaaatcc gggtaaagaa cgcactcttt
    78901 gggcgccgtg attttattga aagcggcgaa ctgagtggat ggcgggcaca cttcgtccat
    78961 aagcccgatt gccatcatgg tttttccttt aattctattt gccagatgct ggcagtcaat
    79021 gtaaccaagg cgtgtaaata tttcattctc acgctcatga cggggatcaa aaagccggaa
    79081 ataggttttc agttccgcat aagcatctac cgcaagatcc atttcccaaa cccgtttgta
    79141 gtcgcacagg aaggggcaca taggcgccag acgtgcgatt tttggctcca gcgccgcaca
    79201 tgccagggtt aaagcaccgc cctgagacat gcccatagct ccgacccggg tactgtcgac
    79261 ctcatccata tgcatgacga ttgcggcaag ctgtgccgta tcgagaaata cctgacggaa
    79321 ataaagcttt tccggatgat catccaaacc tcgaataatc tgaccgcaat gagtattgcc
    79381 gagaacactg cctgtatcct gagaacgtcc gccctgccct cttgcatcca gtgccgcaac
    79441 agaaaaaccg agagcggcat aacccatttt atccgcccag tctccggagc ttcccgtgta
    79501 tccgtgaaaa agaagcaatg ccgggtgtgg ctgcgatgca tggcgcggcc gaatatactt
    79561 ggcatggatg cgggcgcctt gcacaccggt aaaaaataag tgaaagcatt cagcaaaagg
    79621 agtctgaaag tctgccggaa ccagctcaac agccgggtct atggagcgca tctccatcag
    79681 cgctttgtcc cagtagtcat cgaaatccgt cggtttcgga ttgcggccct gataactttt
    79741 cagctgatcc agagacagtt caaaaacagc cataatcctc ttgctttctg aaatcgtggg
    79801 ataggtattg ccccggttca aggctccatg gaagccagta catccgccag ttctgcatag
    79861 ttattgcaga tggcatctgg cacatgcccg tccaaaggtc tgtccttaac gacggtaaga
    79921 aggagtcccc gggcgcctac cgcatgaggc ccgccaatat cattatgggg cctgtctcct
    79981 atatgaacaa tttcatccac ctgaataccg aaatgactgg caacggcttc aaaaagagac
    80041 ggatgcggct tgctgtgacc gatttcatcg gagaagacaa agtaatcgaa atacttcagc
    80101 atgtcatgtt ttgccagcag ttcacgaaga tattttccgg gtgaaaaaat ggcatcggac
    80161 acgaccgcga gattgtattt ttctttcaaa acagccagag attcccgaac acccggggcc
    80221 atgtcgggag aaatatccac ttccatacgt tcaataccat ccacaacacg ggtcaatgat
    80281 tgttcggtca gagtacgtcc gagcccggca agaaccactt caatgcgttc tttggttgtc
    80341 caggtgacgt actgttcatg ccacaccttg cgaaaagcag catctgctgt gtcaaaggca
    80401 tgatcaacca gcagacggtc aatgggtgct tcggtcgcca aggcttcatg cagcaactgg
    80461 cgcctcgcta cttttttggg aggcagaccc atgaaagcac gttttggctc atccgtttca
    80521 tcacagaaca tgcagtccca aagatcaaaa gtcacggcac caatcgcttt ttttgtcatt
    80581 gaacttctcc cgtgttatcc atacagcaca tcttttgtgc ttcggctgtt ttcatggtgt
    80641 tgttcaggta ttttctggcg tttctagagt gtaaaagaaa tgaaagcagt ttatcaaac
//
LOCUS       DAOPWB010000003        63374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_411_length_63374_cov_5.515375, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000003 DAOPWB010000000
VERSION     DAOPWB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 63374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63374
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_63374_cov_5.515375"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKN92_00675"
     CDS             <1..461
                     /locus_tag="PKN92_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNZ47333.1"
                     /translation="INDINTAAWLLDNVPLYVPFQRIVQKEDNRISAKKALSAAEMNV
                     PQYTRSGWQPSFHAPGKKSSAEMQTNPEIPASAPTVPEEGNASGSGKVNLNTASLAEL
                     QTLPGIGPKTAEKIDNYRQSQPFRSIEELQQVHGIGPKKLEAVRLLITVD"
     gene            520..609
                     /locus_tag="PKN92_00680"
     tRNA            520..609
                     /locus_tag="PKN92_00680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:554..556,aa:Ser,seq:gga)
     gene            704..1768
                     /locus_tag="PKN92_00685"
     CDS             704..1768
                     /locus_tag="PKN92_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNZ47334.1"
                     /translation="MLNGVPRILIIRFGAIGDVVRVLPALHALRDAFPHAQIDWAVEP
                     KSADVITDHPALDQIHVFERSGSFWAGIKAFRGFCRHLRNSRYDMVLDFHGILKSGLA
                     MRATRAASRIAFSAPRSQELSHLFANRRVTLPKQILNRIEENLILAAAAGASGRGLEV
                     TLAVSDEIQESVESYVHDTFQSAKKLVIVHAPVDRPEKQWPLSYYAALVDMLLSDGRF
                     DVLLTWGPGQRDIVEKIHAKCKRDPVIAPEMPSLKYFAWLIRQAALYFGGDTGPMHIA
                     SAMDTPVVAVFGGTSPQRHHPFREPCRVLYAGSPDRTENLSPQEGQKLLEKITSEEAY
                     DACTAIIKKSEGGGRPAIPV"
     gene            complement(1755..2846)
                     /locus_tag="PKN92_00690"
     CDS             complement(1755..2846)
                     /locus_tag="PKN92_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ47335.1"
                     /translation="MQYKEFGRTGHLVSRIGFGGMRLESPEDIDGMAEVVLHAFEQGI
                     NYFDTAPDYLDFQSELIFGRAIGEIKKQGKPFFISTKTMAADPDAVRRQCEASLERLG
                     IDTIDFYHIWCLVKPEDLPARKKKGVLKIFQQLKEEGLIRHICVSTHLEHGRVAAMLE
                     EGEDLFDSMLIGLNVQNSHLRLEGVRAAAAKNMAVVTMNTLGGGVLTDHSDRFQAVLR
                     PGDRSILDAAIRFNLSIPEVTVALVGFRNKKDIDQVIDTVNRLQTLSQDEIHEISTAM
                     ASAYKDMCTQCGYCSDCPEGIPVVRLMESYNYGMLKGMDAARRHMQYHWNSPDIKALL
                     ADCTQCRLCEEQCTQHLPILERFEILKQE"
     gene            complement(2859..3794)
                     /locus_tag="PKN92_00695"
     CDS             complement(2859..3794)
                     /locus_tag="PKN92_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ47336.1"
                     /translation="MSKILIVDDDVDLAELVRTKLTKEGHQAFAINTGDGAFEYAKQL
                     KPDVILLDIMLPGQTGYQICRRIRKDPELYRSAVLMLTALGEEPEIVHGMEQGADDYL
                     VKPFKLERLMDKITSLNALLASLGHVNRISNMMGTEAFKRELNHQLARDSAIACVYLD
                     VVGFEAFCRSRSAELQEQALKSIAGLLTALIRSQGFYETAVAHMGRQHFVIMLKLEDY
                     ERFTGLLAQTFDEEVKQLYKPEELERGYITAMTRDGKEVQAPIMALAIGVAHNKFRVF
                     KSAKKMFEVLAQVRQKAQPIDGTSTIFVDRRHTNR"
     gene            4042..5109
                     /locus_tag="PKN92_00700"
     CDS             4042..5109
                     /locus_tag="PKN92_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47337.1"
                     /translation="MTNTARPLKVGLIGSGAIAREQHLPYWRELEEEGRVRIVGVCDL
                     IRERREKESALCANAVPYADYKKMLQKESFDIIDVCTQNRVHAPATIAALNAGAHVLV
                     EKPMAMSSRECDAMIAASKKAERKLMVAQHMRFEARAEKLKEVVDSGALGEIYTAETK
                     WLRRRGIPGWGQFHIKKESLGGPLIDIGVHMMDLCVWLMGCPKPVAASGKVYRKFGDR
                     KDLFNADWGVHYPAEEFDVEDYATALIRFEKGITMQMSVSWAANVHDEIEDMYILGDK
                     GGIGINPLGYFSADHSSLTRTTWDWLSAEDGHRREVRHFCECVEKDLPVMVKPEESLR
                     IQKIIDAIYESSRLNKEVMIK"
     gene            complement(5187..5741)
                     /locus_tag="PKN92_00705"
     CDS             complement(5187..5741)
                     /locus_tag="PKN92_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47338.1"
                     /translation="MSKTLTATVLLVLLAISLSSHAVPPAYEGQFGNPEEPALRVVKW
                     PWLGFRKLVSRTHDGLHRGIYKHPPASICEGTKGAVVGTGVFIDHTARGMVYAPLPPK
                     GPLKKKRTYEDNATLFIEMTLNQQGNTGDLSAAQELAGEPKMLEVREIKGDVRRAQRR
                     YVPHLMSYRDRTPAGQGNLLRLAK"
     gene            complement(5851..6756)
                     /locus_tag="PKN92_00710"
     CDS             complement(5851..6756)
                     /locus_tag="PKN92_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47339.1"
                     /translation="MPFIAVSQSAEIPATAIVYLPAWWKIAILIAVLLELTGISFFIK
                     KKWLENQEALPVKVALTLMFMVFSIYFLVGTVLYWGSPKDERAPLLMWLILFIIPVSV
                     QYSLTLVNALSWRGVQRLTPVGTKIPEAPAIIQTRLLLQAGKITEAQDVLTELFQDRS
                     RALEFTAHWFREEGNYEKAASLLEELIELPVKDPDFWSEAAYSLGKLYESFLNQPLKT
                     MSLFQRIAAECPGSRFCSLAEADLARLRLIYSDQGESDTVAVPAVPGSDSEFWERRRV
                     LLRRQMPPASSAADQPKEAPTDIRQ"
     gene            7103..7762
                     /locus_tag="PKN92_00715"
     CDS             7103..7762
                     /locus_tag="PKN92_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNZ47340.1"
                     /translation="MKERIALNLNTIGCRIKEAAERVNRSAEDVTLVAVTKSVGLDEI
                     LALYELGIRDFGENRLDVALEKIKQCPSDIRWHYLAPVQTRKARDIAARFTVADAVDR
                     VRAAEVLQKRCEELDCHLEILVEVNVSGEESKHGFDESLLSEAVERIRRMDRLSLQGL
                     MTMAPWEAEEKVLRRCFAGLKKLCVAHNLPVCSMGMTDDFEIAIEEGSTQVRIGRALF
                     V"
     gene            7828..8838
                     /gene="pheS"
                     /locus_tag="PKN92_00720"
     CDS             7828..8838
                     /gene="pheS"
                     /locus_tag="PKN92_00720"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNZ47341.1"
                     /translation="MKEELKQLELDGSARIAEASDLKSLEEVRVAYLGRKGRITEILK
                     GLGSASPEDRRSLGQMANLLKDQFAQRIDARKTELEAALREQKAQEQRVDMSLPGRAI
                     PPGHVHVLNQIADEISDIFIQMGFQLALGPDVETEYYNFDALNTPDDHPARDSHDTFF
                     VKPGVVLRTQTSPVQIRVMEQTRPPVAVIAPGRVYRVDLDATHSPMFVQMEGLLVDKG
                     ITFSDLKGTLMHFIHSFFGPDIQVRFRPHFFPFTEPSAEVDILWTARDRHTGEVKSKW
                     LEILGCGMVHPEVFKNVSYDYEEYSGYAFGLGIDRIAMVRHGISSITHLYENDMRFLE
                     QF"
     gene            8840..11233
                     /gene="pheT"
                     /locus_tag="PKN92_00725"
     CDS             8840..11233
                     /gene="pheT"
                     /locus_tag="PKN92_00725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNZ47342.1"
                     /translation="MQISLNWLKELLPLEADADEIAEHLTMLGLEIEAVRRPGQDIEN
                     VFVGRILDIQPHPEADKLVVCRTDAGQGEALQIICGATNMKVGDKVPTAIDGATLPGG
                     FKIGSRKMRGIQSCGMMCSARELGLGEEHAGLLILDKNAPIGQDIRDFLGLNDVVFEI
                     EVTPNRGDWASMIGVARELAAFYGLQMTLPDAEVKESGEPAAAWSSITIEAPDLCPRY
                     AGRILRNVVIKPSPPWLCARLIAAGQRPINNVVDITNYILMETGHPLHAFDYEKLKEN
                     RIVVRRARADEKITTLDGTERQLQEDMLVIADAENAQAVAGIMGGADSEVEKTTTTIL
                     LESAWFESRCVRSTSRRLSLSTEASQRFQRGADMQMLPYALNRAARLMQELADAEVAP
                     GILDAWPQQRAYPAVTLRYERTVSRVGVNVAPAEQKRFLVSLGCELESETDASATFKI
                     PSWRHDIRHEADLIEEVARLFGYENIPVTMPPVRQAESVFAPHATILRNFRRYLAGQG
                     LTEVMNWSFGSPEGAVDAGLPVSPADQVYLSNPLSENYAVMRFSLLPGLFGTAAYNAK
                     RGRRDLRIFELGPVYRNSADEILPAEPMYAGILLSGNAPGHWSRAECSYDFYDIKGCL
                     DVIARFFGKELTLEESASSGFQKGQFAEIKWRKHILGCIGKVADEVVKTFGLPENTYV
                     LEINLEILLAKKTEPAQFTAIPQYPASLRDLAVVVDRAVPAKDLVAATKQAAGPLLTA
                     VNVFDVYSGKPLPSDKKSIALSLLFQSPDRTLTDKDTQKTMDRIVSRLRKDFEAELR"
     gene            11230..11526
                     /locus_tag="PKN92_00730"
     CDS             11230..11526
                     /locus_tag="PKN92_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47343.1"
                     /translation="MNQFLQNYIEQINVLADQLEKTLTSYGTQLASSRIKNEEDQKEK
                     WMMARKIKTMEENLDNLAELKQENEELRQKNMQAAEHAKRILILTKSLADALRL"
     gene            11523..11828
                     /gene="zapA"
                     /locus_tag="PKN92_00735"
     CDS             11523..11828
                     /gene="zapA"
                     /locus_tag="PKN92_00735"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNZ47344.1"
                     /translation="MSILNPKQQVEIAGNRLQVPLYIDEETTEQLAAEISARLKKIGA
                     TYETVNTQRNALATAYDYAVETAETKKQYEQDVREILTALERITSSLKELLDRFPPV"
     gene            complement(11860..13230)
                     /locus_tag="PKN92_00740"
     CDS             complement(11860..13230)
                     /locus_tag="PKN92_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47345.1"
                     /translation="MQPVGLSYRQAATPDEIHLLPRLIFQQMHRWFGDSIPGYLVVNF
                     LLLYGCMVLIFFLTRLITRGPWWLGSVSAVLFMANPIKSTALLSIGGIQWLLPGLLGL
                     AVLLLYALCRSGSMIGHKWLPFLTYLLVSVFTPSNIPLFAVIFVLEEICFKKAERSVT
                     RHHIYIAAAGILLLFFSGQIFLPHALSLHAMFIPLLLLLNPIGFLSSTFSFFSYYTIA
                     FYIGLAAGLTLLCFFMRRYRHPAFIFGILGAFAFRIGQGSLPIDLVTLENSSTLLIPT
                     ALAAVGAAGFFLALSEIPHWNTSVVRLSTLVCCAAMLCQIWVNLQWYRAGADIARFRT
                     AARISAEEHPGQYLAVFPDIDYVGFVPYFLSETLRPIDRNTSALPVYSPASFSVMPPL
                     RAEVLVYAPNLAVASITGFDEKGKPDFRPLSHDWWRQRLFRSGSRVLTREAGDHLFPL
                     HRIPFP"
     gene            13760..14980
                     /gene="pilM"
                     /locus_tag="PKN92_00745"
     CDS             13760..14980
                     /gene="pilM"
                     /locus_tag="PKN92_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNZ47346.1"
                     /translation="MGFRKPRKVIGVDIGTHAVKSVLMTRISGRLKIEQAAMCPIDRN
                     LLNLDPVKAQEEAVRRTLERMPVQQSLIVGALAGQTVVVRYPRLTDVSKERLQGAVAK
                     EATNSIPYDLKDVFLDWVVLEEYSEEGRHITRVLLVAARKEVVDVRLQVMRAAGLECG
                     VLGVDSLALADAAEACDFFQKGETVALINIGLNSSSIQFVKNGVSNFIRDVNWGAREL
                     IQSVAKGMRSNYEQALSTIENYAIDHVSVPEAQEAGGAESSFAYMETPFLEDIPQAEA
                     YMGGHDDEQGYMESELSYMDSGYGKSATLSDLVASPLNRLGVELRRSFDYYEHQLYEQ
                     PVDRILISGGPVAFAPLVESLQEVLNFSDIEIVNPAASALLVSEEASLSAFHAHPAQF
                     AVAVGLAARGMAEL"
     gene            14994..15656
                     /locus_tag="PKN92_00750"
     CDS             14994..15656
                     /locus_tag="PKN92_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47347.1"
                     /translation="MIKINLLPKELRPVQRTPIPHIVSLLILAGALVFMYNIFIGQRT
                     ELLIVDGKVQKMTKELDGLQEAVEEHKELIKQKSLLQRKILAIQEILEGKTVWSEQLH
                     RLATLTPENIWYKRIRITSKKFAEEKPTLDKKTGAPVVDAKTGAVRMTRVSVDKQILE
                     VSGYAIDDEHGLSSTSTLAARTTNDKDFSNYFMLYNSKIVDTDFNGHPVREFTFEYII
                     GG"
     gene            15716..16291
                     /gene="pilO"
                     /locus_tag="PKN92_00755"
     CDS             15716..16291
                     /gene="pilO"
                     /locus_tag="PKN92_00755"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNZ47348.1"
                     /translation="MAVLAKGKITRQDWAFVGITLGVTALLFVAFYLFGYTKLQNRIK
                     AQEAEAVRISKELKEAHALTDGIEDLRKEAQEMNHLVESFEDRLPEERKIPALLARFE
                     KLGYEMGLRVQLSSLPTRKTLNNDMEVIPYKAITDGQFHQIVSFINMLERDERYLKIS
                     DLDIDEEIEGISKATFVLSTFRFLNEPEIKE"
     gene            16288..16860
                     /locus_tag="PKN92_00760"
     CDS             16288..16860
                     /locus_tag="PKN92_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47349.1"
                     /translation="MNEQNKKQIIMAAVLGVVLIGVLVYQFGLSGSSSGGRSADTKTA
                     EKSKTTSKKSSSNAKGAPVADAAALASIDIAALIATVEVVPFDYALSRTARDPMAPLV
                     GNLPPGLAAESAAADETDALAYVQRGTTATRVVSGIIWDKQFPVAIVDDIVVHVGYEF
                     PNGAVVHSIEPLRVLFKVGEAIIPVEMKEF"
     gene            16864..19128
                     /locus_tag="PKN92_00765"
     CDS             16864..19128
                     /locus_tag="PKN92_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNZ47350.1"
                     /translation="MRYHRHVKAVGLSVAVLLLAAVAWAEMELQAKPSALLESSAPLA
                     QQVIQLPANNSPGTAHAQKADMLKSMLVDFVNEQEMTATKDPEAVSLQIIDKDLGPVS
                     GMEQKVLAVLQMNDPSEVADTYEAADGVDNQNAAAPGDDAAADAPDLPEREPAPKVDA
                     AQEVAEEEEGPLPPAPRVQTSQDVKDANASLVSQLRTLSDGSETPGGGSVTAGAPSGS
                     SDTALFSKDGSDPAQAPNRAPKSGSLNDLVNLDFRNTDLSHVVAILALKANINIIAGA
                     DLKGSVTANLYQVPLHVAMETVLRMNGLGLIEEDGIFFVVPYEEAASVNRKTVMVAMD
                     NANVDDVRKVLTDVTKGAREEAQISISSNKATNVLIISAPKARVDELAAMAHQLDVAE
                     PVLPTETRAISLNYCEPQKLVPVIDKMLSPRIGKASADERARHLIITDLPVVVEQISD
                     LIKTLDIPTKQVLIDSMVVEALLSDDADTGVKWLMNSLHRMSRRQALLGEEGKSLGNI
                     QELALGADLEALRQPAGMLTFSLLGDHIDWSGLIQAEVRNRNGRLLSNPMLLTVENES
                     ANISIAQEIPYIELSQTNAGGSQTNTRFKEIGTVLTVTPRVTHDNTIICKLEGKESYV
                     SGEFQGVPIEDKREVASTMRMQTGQTIFIGGLRKSQGTSTAKKIPVLGDLPIINFAFR
                     SNSRTEQINDLLVFLTCSVVNEQDYQLSPHQQQVLEDVPPKEMKADAWETTLYDTRHP
                     QAVKELQWKWRREP"
     gene            complement(19242..19838)
                     /locus_tag="PKN92_00770"
     CDS             complement(19242..19838)
                     /locus_tag="PKN92_00770"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNZ47351.1"
                     /translation="MSEPFSSKSEARLFYLEKRRSLSSEDWQAKSGSIFQRIINSSFY
                     QESNAVLCYISSKDNEVDTHALLAIAAKSGKRVLVPYTAHCLPGTMRWAEMQPGAELV
                     RSAFGLLEPAPGTVALRTPPPGSLCIIPGIAFTREGHRLGYGGGYYDRFLKSHKGVRV
                     ALGFDLQIADSLPFDNFDETVHYLVTETEVIHASKESR"
     gene            complement(20225..21685)
                     /locus_tag="PKN92_00775"
     CDS             complement(20225..21685)
                     /locus_tag="PKN92_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNZ47352.1"
                     /translation="MSSSNRSDYSAALPWRRILIVIIVTGALAAGATLGVTGLLINIF
                     QRQQEAKNPFMRVVEVTDETTDPAQWGMNFPMQYDQYKQTVDMERTNFGGSEAVPHTP
                     TDEDPRTVTAKSKLETIPLLKRLWAGYAFSIDYREKRGHAYMLDDQTFTQRQKVQQYG
                     NCMNCHASMYTVYKEIGNGDLQAGFEKINPMKYQDARKLVEHPVACIDCHDPETMQLR
                     ITRPSFMTGIRAAKLAEEGIADYDVNTMATRQEMRTFVCAQCHVEYYFAPDESRRLTF
                     PWYKGRRADQILDYYSEIGFTDWTHKETGAQMLKAQHPEFEMWSQGTHSAAGVACADC
                     HMPYMRQGGLKISDHHVRSPLLNINNSCQTCHKVSEEELLQRVNRIQERTVEMCNMAL
                     EGLATFIDDLVEAKDGGLAEAQLHEAYQCQRGASFLIDIVQSENSHGFHAPQESARLL
                     FKAMDYLRRGEIALQAKEIAAKEEAPSSLEATETAS"
     gene            complement(21732..22211)
                     /gene="nrfH"
                     /locus_tag="PKN92_00780"
     CDS             complement(21732..22211)
                     /gene="nrfH"
                     /locus_tag="PKN92_00780"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNZ47353.1"
                     /translation="MADFLQHTWLRRLFYASLFFGISSGLGLGVGTYTFFYAKGLSYM
                     NDDAETCANCHVMKEYYDGWVKSSHKAVAVCNDCHTPHNFFGKYWVKAMNGMHHSYAF
                     TTGFFPDNIQIRPGNRQVTEGACRYCHSKIVEEVDRFGHGPQALDCIRCHTSVGHLK"
     gene            complement(22422..23483)
                     /gene="rlmN"
                     /locus_tag="PKN92_00785"
     CDS             complement(22422..23483)
                     /gene="rlmN"
                     /locus_tag="PKN92_00785"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNZ47354.1"
                     /translation="MTAAFEAFHLTGSTPEEMAELLSEPPRLGRQIFTWIHRKRVFNC
                     SAMTDLPLQLRNRLNAQETLFALKVIEKKQSKQNGTCKLLLECGDGELIESVLLRHKD
                     RLTFCLSSQAGCALNCSFCATGQSGFRRSLSAAEMVEQALHLACAGNLQSNSTPNLVY
                     MGMGEPFQNYDAVMKSIALLMNPLGMGIGARKITISTAGDIPGIDRLAEEPRQLRLSV
                     SLHAAHDDLRSALVPLNKKYPLQDLHDALQRYQNRRGRQITIEWTLLENTNDSPEEAL
                     RLVSFLKGLDAVVNVIPWNPVSGLPFTPSPQKRQDRFMSVLEHAGITATLRQERGGDI
                     DAACGQLRMRRNRSFREKS"
     gene            complement(23480..24445)
                     /locus_tag="PKN92_00790"
     CDS             complement(23480..24445)
                     /locus_tag="PKN92_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47355.1"
                     /translation="MTKHNACFTRYALRPVRPFLTVCKPSALLWLLPAVLLVSLTGCV
                     HLGTPCEPVELPAGAPSVRDVLASLTENEARLHNFKATGTIMVKLPEMEGIQVSRESV
                     VSFAAPDSLYVLGRRYGTRILNLIYDKDSFLIEFPTRREYYYRPSEESLGSVTTADIV
                     REMFQPEDWKQLNPRLIQMTAYDEEKQTATLVLWEGSGVALRHKRTLKLQGAPWVVLE
                     NILYNESGVPVARTNKSHYYNNNDLHYPTQIECTFPQEDVWMRFIMRKVETNGEIDPA
                     LFDIPAKLSDLIYKKHKQVDLFRSDITSLDDLSAGTAEENQDPVP"
     gene            complement(24432..25673)
                     /locus_tag="PKN92_00795"
     CDS             complement(24432..25673)
                     /locus_tag="PKN92_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ47356.1"
                     /translation="MDIFIFLMCVLGSFFFAAYETCFYSANLIRIRHLAEKENNKTAV
                     RLLAHYKDPGRLITLMLVGTNLVLVIGTISLSRAVGAGWTTLIATPVYLFVGEIVPKS
                     IARHFPMQMVLPLFPLVTFFDRLLAPITWPIVQLSKLVLKMLGREGEHMKPLAGSRED
                     VRILVDESHDQGALDPEEHEMIHSVIALQNQSAKEVMVPRMQMLALPESATRHELTAF
                     FIESGRTRLPVYADTVDQIVGVVNAFDLIKDTRTDRQDIARFIKPIIHVPDTMKLDDV
                     LKVMRDARQSVAIVTDEHGGTDGLITLEDILEQIFGEIHDEYDLAPTQLKKIGPDAYF
                     VDAQMPLEAFCEALHIPLIENGVETVGGWVTRLAGCIPERGQVIPWENHTIIILEGAP
                     NYISSIRLELNQEKAPEHDQA"
     gene            complement(25683..27149)
                     /locus_tag="PKN92_00800"
     CDS             complement(25683..27149)
                     /locus_tag="PKN92_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNZ47357.1"
                     /translation="MDDDTSGDPPRIARLGFTGRSAFQAFVLIFSLLLFCSLATRANG
                     AADTGGTGHFSHPWHEIITPSVIFASFFLILCSFFFSSCEASFLSLSKVQLRALRETG
                     SWIHGLTAQLLDKPASLLTTILMGNTIVNVLLSITFAGPMTAVLQKSFLLSGTSAYLL
                     TVAVTTAILLFFCESLPKMLAVAHPRTFAIAAAVPIYCVEMLMTPFRLFSMSLVGYLF
                     KVTRLSQVPPAVFLTDEEFKILLSEGEAAGAIEEDERQMIQGILEFQDVTLREILSPR
                     PDIVAIRESATVREALAIVREHEYSRMPVYKDSLDRICGILYAKDLLPVTEDGEQDSP
                     VTQYMRRAHFVPETMTVADFVSMAQRLHIHIAIVVDEYGGTEGLVTLQDALREVVGDI
                     GEEDDVEPPLCVDGPDNSKLIAGNFPLSDFAEMMGLTLKNEEHTTLGGFLQSQSETIL
                     AVGDHIAYEGLCFRVEEIDKKRITLVRVMHEPCTAPEE"
     gene            complement(27210..27665)
                     /gene="ybeY"
                     /locus_tag="PKN92_00805"
     CDS             complement(27210..27665)
                     /gene="ybeY"
                     /locus_tag="PKN92_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNZ47358.1"
                     /translation="MITLDIKNESTVHRIIQKKTLLRLLECIVTGEGIKDPLEVSVLF
                     CDDAFMTSLNKQYRNVASTTDVLAFPMDAQIPSDSVRVLGDIVVSMDVLRTRCKDVRE
                     AMRQELLLLVCHGMLHLLGYDHDTPEKRTLMQQKQALYLELPFDTVWHS"
     gene            complement(27662..30178)
                     /locus_tag="PKN92_00810"
     CDS             complement(27662..30178)
                     /locus_tag="PKN92_00810"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNZ47359.1"
                     /translation="MAFSSNINTPTGLSTGADSVIFNVHNGQDHTEKRIFQVPLRQIA
                     FVLAVFVLSIAALTRESPRDRISPEDLKSTVAQREIRAMFYFESTDIKKTQEARDLEA
                     MKVPVHYRIHNEAAEEQIRLLQYRLEMIREQCSLVPEKLHAALQRSEPAQTPESVLQR
                     EVTAYATRLKEETAWESFPEPDLLALWLTPDKVNLTSAPGADLSAPRDPTALQPEFTF
                     EQSNKMGAMALSSLRFVLFTGIRHSVSSNIDLARHIVILRDRNLPELPITTDTSFGEV
                     PDLAKAATLLNQRLTESAKTLASEMGMPEAWARIHDASQALVEPLLIATLQEDRQSTE
                     NARAVAMESVAPVLKEIEAGEIIQDAGKRWTEQSRIDAQSYMHVLSDEQHPYKKILNT
                     LTAHFILVLLVFWALYKRLHFQVDQRGLSSRTVFNLSLLILCGTLIIGRISSYFEPSG
                     YVLPVAAGGILFAILAGPQRAAFFGVLLAAMISAQYQYNWRLLLVQGAMIIAGAFSIY
                     KVRKRNDMTAAALIATFVGLLCAGAGLLASDLLFGEIFMRRSFLILLNGGLCVLVVPA
                     LLPWLERLFDITTDMQLLEYSDLNNELLRKLAIAAPASYAHSLLLGQIAEAAADAIGA
                     NGLQARVCAYYHDIGKRHKPEHFTENQGGRKNIHDNMPPEISARMIRQHVIEGAREAR
                     EHNLPKPVIDGILEHHGTCRISFFYDLAQKTNPGEIIADDEFRYPGPKPQCPETAILM
                     ICDASESGARSIPNPTQEKVRQFVDTIIKARAEDNQFDNCDLTLKQLRIIRDVVARSI
                     FNAMHTRIAYPGQNAHKKEEPQPLQDNTSQKNSQEASVPT"
     gene            complement(30258..31199)
                     /locus_tag="PKN92_00815"
     CDS             complement(30258..31199)
                     /locus_tag="PKN92_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNZ47360.1"
                     /translation="MALLGHNGAIRKLVQTHFNVRIVDRGAKVTILGEQNAARSAHEL
                     LSGMLDAVRKGYSLTLKDFEYALTASPDTAGTTTGELLSAKPAGIRPDVKVRPRTDGQ
                     RLYLDTIYAQDITLVTGPAGTGKTFLAMAVAVSALLNRQVRRLVLTRPAVEAGERLGF
                     LPGDLTQKVNPYLRPLYDALYSMVDMDRIGRLIDQEAIEVAPLAFMRGRTLDNAFVIL
                     DEAQNTTPEQMMMFLTRLGQSSKMVVTGDVTQIDLPRGFKSGLIDAQETLQGIDGIGM
                     VALTRSDVIRNPLVQKVVSAYEARQLQNKVRFEEKTE"
     gene            31439..32668
                     /locus_tag="PKN92_00820"
     CDS             31439..32668
                     /locus_tag="PKN92_00820"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNZ47361.1"
                     /translation="MTIELPRLKSYSRMKEVMTMYYMGAKMAEGSGQKMAWITSGAPV
                     EFLYAADITPLYPENHAAMCGASRLANTLCSAAEEAGFSRDLCSYARTDIGAILTGTS
                     PIGGLPKPDLLVCCNNICGTVTKWYQELQRHFDVPLVLLDVPYQYEKEDAAHVVSYLK
                     DQLKGYIGTIEEVTGKSFDYDRFDEVVALSGEAADLWRSIQGMLAHKPAPMNSFDTFI
                     HMAPIVTLRGTQLCLDYYRELEAELRERIEQGIGAVPVERFRLGWDNLAIWHQMNQLS
                     RKFAEFDAALVVSTYTENFSYRGPIQDPNDHLLTYASTYIGGYINHGLDFREKELTRM
                     VKDFSLDGFVMHSNRSCRAYSFGQYELARRMEEKHGVPTLMLEADMNDSRTWSDEQMN
                     MRIAAFMETLTGRCAVM"
     gene            complement(32732..33784)
                     /locus_tag="PKN92_00825"
     CDS             complement(32732..33784)
                     /locus_tag="PKN92_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ47362.1"
                     /translation="MRKNSFFLPSRTVIAGCLFLSLLLPCLPDVTASVYAELIEGEPE
                     LHALPEAPALPPDIHSESDLYIAAAKDEITLLNPQGPVPVPGNVLEKKDIEYGNVNGR
                     ALLLDLYLPRDQDADIPGILFIHGGGWASGNRSDYKYYTVRLAQMGYAVATISYRFVS
                     EASFPACVEDAKCAVRWMRAHAKEYRICPDTIAVAGGSAGGHLAMMTGYSSDIEALEG
                     TGGYPEFSSAVQAVINLYGPTDLTPVEFHDHPTLKAFFGGKTFNEAPESYALASPIRH
                     LDKSDPPTLVIHGTDDSIVPVAQADLLVARLEALEIPHEYLRLEGYPHTLDIIQEVNQ
                     CVRWHMYRFLEKYLKK"
     gene            complement(33856..36429)
                     /locus_tag="PKN92_00830"
     CDS             complement(33856..36429)
                     /locus_tag="PKN92_00830"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNZ47363.1"
                     /translation="MPTARSLRFAILPLLFLSGVLCASALPATADTAASLEEIWFDSW
                     TVEKSNTVTIDLPVLPSVPAADNIDLGISIAATVPYQKTAVILQVRDVQNNLAQRLES
                     IADLRQGLNDFRFEWSGLSLTPGRYTLSIQVDYSDAFPPLSAHTCMRRISSEAWQQKL
                     AQSIETLSKLEQQSTALSEGIAKKHGQSLVSTPYLSLSVQTAHRAIEAAKDALQQGNW
                     SVVSRNMEYLETALPSLQAALVFSTQRPELCMTETTDHLTCELRQGGFYCSDKALFLF
                     GLGLPNPETTTEEQAFLENQAALLSRYNLNFLVLNCPITLDKSQIASLLDRAASAAEH
                     EQLFWALQFQQDEDVEKLMDVQPAIISPGFVNLAHVDFNKAYRDQLLAAMPHLQNRSG
                     RIAAVSLADNPRFHYDGEEVRQAFINTIKEKHPDRMTLNRLWHAKLASYDEISIWENP
                     KEHSYHNQRAYQYEWQTFHRSLIADFFSQFKQSLSSLAPKIPLTLTQPDTAFLPGETR
                     STAGRSDMSEIMDLQACTVRFEPGDGVYALNYPIPNAYLTLMRFYQPDKALLPLKGDI
                     DVSTIKESEMRVRIVTAALWESVISGASGFVLAHDSSVFDYPDAFEAFVRSTMDINRL
                     GNIVVAFQQDTPEIGILFSEAAKIMDDGVPHLESARFAFEGSSFSGYPIHYLTEGQIL
                     KGGLDKLKVLIMPETMAISDEAFLKIDAYVEAGGMVARVGTPIPYNESGISRNDVIRT
                     SPNTVLVRGMNLPTEYLHAMDAVLDRGKLPLIPRPINAHGYPLEGVRSRYVRIQNQEY
                     LYIINLRRAPVGVHITGASEGGRDLILARDVEFPREMEPLEVMLVQLDSPAPADLSVQ
                     E"
     gene            complement(36550..37728)
                     /locus_tag="PKN92_00835"
     CDS             complement(36550..37728)
                     /locus_tag="PKN92_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47364.1"
                     /translation="MKRQMFLSLVFVCAVALYGHAATFQEDVDFLKQYLDVIVLKSPD
                     GQAQVAVVPAYQGRVMTSTASGANGPSFGWINRELIASKKFEPHINAFGGEDRFWLGP
                     EGGQFSIFFEAGAPFDLEHWQTPPVIDTVAYAVVEQSDSEVAFEHRATITNRSGSSFD
                     VKIRRVIRLAESLTDEAQSVAFESENTITNLGDSAWNEETGMLSIWILGMFSPSPDAT
                     IVIPFREGSDEALGAKVNDKYFGKVPEDRLVQRDNVLFFSADGTMRSKIGISPKRATE
                     WLGSYDSGLQALTLVQYTLPGDTDKYVNSMWELQEEPFSGDAINAYNDGPPAPGKKPM
                     GPFYELESSSPAASLQPGESLTHIHRTQHLIGPKEALEPLAVKYLSAKLDDISSALPR
                     "
     gene            complement(37778..39355)
                     /locus_tag="PKN92_00840"
     CDS             complement(37778..39355)
                     /locus_tag="PKN92_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNZ47365.1"
                     /translation="MAETKRFLWQCVEHWASVKGDAEALVFEDTRLSWAELARQVDQA
                     ARAFLALGVGRGDCVACISSARPEFVISFLAASKISALWTGISPRYTVGEMGRILRGC
                     RPTVLITQERYHNTNLLERAFTFNFELASIEHIILIDGEHGDAPSFHTILKKDYSDTA
                     SLYEERLAAASPGDNALLMYTSGSSGNPKGVLHTHSSVISNVSQQHTLYNIHKNSRIL
                     LHFPINHVAADVEISYCALFAGATIVMQPDFNAKASLEIIEKENITVLGQVPAMYILQ
                     LREPSFHLTRWDSVETFVWGGSPASKEMLEALHRICSRTGAILVTGYGSTELCGFVTA
                     TQKKAPFPVSDQSTGPVYPNCELRIVDEQRNPLPPGQIGEIAVRGPVVMKGYLNEPIQ
                     TAAVLDAAGWYYTHDLGSLDKEGNLTLHGRRTDMIKTGGENIFPFEIELTLESHPQIL
                     FAAITAVPDDVFTEVCHAHVMTVPGSKLTEMQLLSWCRDHLAHFKIPQRVFFYATLPL
                     LPNGKVDKLKLVEINPL"
     gene            complement(39589..40890)
                     /locus_tag="PKN92_00845"
     CDS             complement(39589..40890)
                     /locus_tag="PKN92_00845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNZ47366.1"
                     /translation="MKITILGCGYQGLVTGTCFAENGHSVVCLDKNPAVINQLQAGKL
                     PIHEPGLEELIIRNLEEERLSFTTDLSAALDGSLMVFLCVGSPADRLGQVDLSNVLSV
                     TEEIAKKRTGYQIIVNKSTCPPGTAKKIEEIWKIRQAGDVVVNPDFMKEGSAVDDFLR
                     PDRIIVGCRDVRVQEMMRELYSPFLRTGKPFLAMSPESAEMAKYATNAMLASRISLMN
                     QLADLCEAYDCDIADIREAVGMDSRIGPTFLFPGIGFGGSGLPRDVAVCERLAREAGL
                     EHDLLTAISAVNNRRRQQFLAKILDYYGSKISSKRLALWGVSFKARTDDIRDAPSLDI
                     IEGLCAAGAEVVVYDPVAGQKVSARYGNRIQMAPKYYSALENADGLVILTEWNEFRRP
                     DYARMASLLREPVIFDGRNLYTPVVMQEHGFKYFSIGRKSV"
     gene            41208..43172
                     /locus_tag="PKN92_00850"
     CDS             41208..43172
                     /locus_tag="PKN92_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNZ47367.1"
                     /translation="MRKHRRLIFFFIFIFIGIPFVFMWGKPTSPHGNQAADSAVLAQV
                     GGVPIKESEFRRGLDAAAQARSRQGGDRPSYTDLDKDGTAQAVLEQLVDASLLRIRAA
                     QRNFSVDKRVLEKQMQQWGMFQDENGVFNHEIWNEWIASVERWNEIYDELEEVVGRQM
                     YLSAVSAPAHRIIAAKAQNELKSDNIKIKVKFAKIEPQVAVSDDDARKHYDENPEAYR
                     LPEQYKAEFIAYSLTPDVPEQANELVARIRAGEDFVAVAKEYSGQDEEDSSDLGWSPV
                     NPDAAPYMKALYALEPGAVSDPVWGPLGYHIFKNEEIRINPDTEEKEVHGRQIVVPAM
                     MTPEEAAIREKALGEIAKKIQDGEDAASAAAAAGLSLQQTSFFGRTSTEIEPLSMNDL
                     FTFRSQVIAQKDTPWTVLKGRDNLFITHIIETAPGTIPEFAEVEQDARDNFIAQEKRS
                     EAYLARIDEYAKQIKESKLKIDSLAGAFPDIVQETGETPEAFTRKDTLFQQKIYVQAN
                     EIVAALQDALPGDIAGPIKGFFGDAWFFELVEKTEPTEEELAALSDELKNIEARLAQN
                     AEYEIMSDFMKDLRERMLASVAYKQNNDVLDRVLSRGKYAIEEREPEKETAPVDAAAS
                     AADSSPGESAGADAAAAAETADASGSEEDK"
     gene            43279..44232
                     /gene="trxB"
                     /locus_tag="PKN92_00855"
     CDS             43279..44232
                     /gene="trxB"
                     /locus_tag="PKN92_00855"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNZ47368.1"
                     /translation="MMEKVVIIGGGPAACTAALYTARADLSPVVLEGEFSQDILPGGQ
                     LMTTTEVENFPGYPEGISGAELMADLKKQAERFGARFEYKTVTRVNFADRPLRLFCGE
                     TEISSHAVIVATGATAKYLGLDAEKRFLNNGVSACATCDGALPRFRNNVVVVVGGGDT
                     AAEEALFLANFAGRVHVIHRRDSFRASKIMSDRVISHPKITVEWNSVVTDILGDDEKG
                     VSAVVLKDTQSGEERTLACVGYFAAIGHKPNTDLFAGVLDMDDAGYLVTRPDSSYTNC
                     EGVFACGDVQDRIYRQAITAAGSGCMAALDCTRWLEAQQID"
     gene            44277..45302
                     /locus_tag="PKN92_00860"
     CDS             44277..45302
                     /locus_tag="PKN92_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ47369.1"
                     /translation="MTVLFSSKLSIREMAVVCRQLSTAYNAGLPIISALQLVVDRGAS
                     IRARRALLTMAELIRNGATLADAARAQEATFPDLFIVVLAAGETGGKLGALLNELADH
                     YEEMAKIARAAKVAMVYPGLQLLSAWYLGTFALNIMKTILDPSRGRIVFADYLSSYAS
                     FQIKSHLIALAVIAVIFILKRFGMLDSGISIVKNHVWPINQISRKFSMARFFQCFALL
                     LGAGIEIKQCISRSAALTLNRIMERDLLQAIPFIVQGKTLAEAFARCKSFTRVDHEMV
                     GVGELSGELEATCKKLSEYHLAEASSALKVGMRVMQVLITLVVGAVIGYIVISFYGRL
                     YGSMLDL"
     gene            45340..45894
                     /locus_tag="PKN92_00865"
     CDS             45340..45894
                     /locus_tag="PKN92_00865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ47370.1"
                     /translation="MMDIQEKLKNWNEETVAEDPRDYTKLIWLGVGILIVAMMFFLYS
                     GRTTKPLTTSVHVRHILVSFDPADPVDRGKAHDRILELKKRLEAGESFDKLARKYSDD
                     TASAKRGGDLGWAPKGIYAAPFDEYCWTGNIGAISDVILTNFGFHLVQIEERYIAGAD
                     LYEKELEKRALEKMKEEAAKGVAP"
     gene            45941..47638
                     /gene="argS"
                     /locus_tag="PKN92_00870"
     CDS             45941..47638
                     /gene="argS"
                     /locus_tag="PKN92_00870"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNZ47371.1"
                     /translation="MILQEMIARLIVSGFPDVEDADIQCTVPPSPELGDVSVPMFQLA
                     KKLRKPPVVLAKEVADSVDFSAIAESVSLAGPYLNLILKRDYFAKQLFSRAFAKKEKW
                     GSNASGAGKKVLIEHTSINPNASPHVGRGRCAMIGDSLVRLLRFEDYDVDVHYYVNDM
                     GRQIALLVLMMEELDNPTFDEILEIYVKANVRAEADPEFAARGYELLARIESGDEEIR
                     KRFFDITEMCLKGQLAVLQRIGAQYDFFDHESNYVNDPRLSVIEKALQSKGALFTDEE
                     ARLVVDLSKLGYPLEEGRFFVLRRANGSSMYGCRDLIYSIEKQEKGADYNLMVLGEDH
                     KLYAQQLALILEAAGYPAPETIYYSYILLKEGKMSTRSGKVVLLTSVLDQAEAIALEK
                     VTEQFPDLAGAEREDIARQVAVAAIRFAVLRVKPSKNVVFDMETALSFQGDTGPYIQY
                     CCARIRSILRKWDQALPDLSSCAFAFDEEGEWRLVLKVADFPDIVKEALLLRSCAGIG
                     NYLIDLAHVFTTFYHQCPVLTAKTEVQQESRILLCALVLQTLSNGLALLGIETPERM"
     gene            47820..50213
                     /locus_tag="PKN92_00875"
     CDS             47820..50213
                     /locus_tag="PKN92_00875"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNZ47372.1"
                     /translation="MIEKRYIDLIASDENVTPEQVESAISLLNAGATVSFIMRYRKDV
                     TGGLSESKLERISDRNKYYTQLYSKKKSLLHSLEKPDPGQEEKQVVSEEIQAAIDACD
                     DMIVLEDLSLPYKKQRNNHAATAVAKGLLPLANYVWAQAPSLPPPEMYAQSFVSADQK
                     IYSVEEALEGAKHILAECIAMNVDVRKELRKALAEEGRLLVHPTRAEAEKKSKYDAYK
                     DLNVLLAELDAEKLLTILRGEWEAALRVELVIDDEKMLAGIINRFVNDKESAYAVELT
                     GAVNDAYRRLLRPSLEVEVIAQARRNAEDAVIEACREQVRNTLLAAPAGEVPVIAVCS
                     LSPGEYSFVLVDESGALLEDRQVESPNEDVLHADAEAALEALLHKAPAAGIAVSSGPG
                     GRSLLRLIQAYLKKKGHRQSYATLVSDAGLAAYASSPLAAEELPGLKESVRAAVSIAR
                     RHQDPLKELIKMDPILLVPGKIAFGVNRRRLLASINRTIESTVNRNGLDVNKSTVEML
                     RYISGLQFGLAQAIVKTREERGGFTNRTQLMEVPGLDNKIFQQCAGFLRIIGGEQILD
                     ASSIHPEAYGTVEKLAASLSLSVAELTQNTEPLNSVRLEDFVDGIIGVMTLEDICYDL
                     GRIGRDSRKKFKAPASFVTLDSIEDLEPGMVLEGLITNVTDFGVFVSLGLPEEGLLHR
                     SEMPRLDIKALKTSILTGEVIKVQVKEVEPSKGKIGLTIRNVAKLPLTRPTGAGRFPK
                     EPWDQRERGRGDRTKRRAPKDSQRAPKQQKEGLHEGSLINTALAEQLAALRNKIISK"
     gene            complement(50289..51830)
                     /locus_tag="PKN92_00880"
     CDS             complement(50289..51830)
                     /locus_tag="PKN92_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47373.1"
                     /translation="MYERQGKFAEALESWKSVLAFAPEAHAPRLRMIRLYLTQGDLNQ
                     AIATGEEVLRYSPEVVEFWVIQGEMKQAAGDFDGARDCFEKAIALKPDNMLGYSALAD
                     LYEKTNDLVAAIELYEKLIQNSPDSAALYFQLGMNLTKINDNAGACAAFTRVLELEPL
                     VTQARLMQAIHLSNLHRFNESKDAFYLYLAERPNDMQALQYLVGTMLHLRRMEEARSI
                     MHRLLENQEAGAKNVLQWALVQGLEMNFSGAAQSALEGNAPFLASCYGRLANAAPNEY
                     SGQDKVFLSLEEIELECSRIEDALISLQAPPQLADALCSQMAFFQESPSIGRFTAFLQ
                     ARLLLYLKRPREALDLLNQIQADGEAQKFVLYHQAIASEALKDMESTEKHLLAYLFLD
                     SQNATMLNFLGYLYAEENRNLGKALKLLTLALSLSPDDPFYLDSLGWIHYRLGNFDQA
                     AELVRKAIYGMETDDAVLRRHLGDIYEAQGNTQAALEQWRRALRLDPAQDDLRKKMEH
                     YQPAN"
     gene            complement(52053..52784)
                     /locus_tag="PKN92_00885"
     CDS             complement(52053..52784)
                     /locus_tag="PKN92_00885"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009658929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNZ47374.1"
                     /translation="MEPYPDLTSFLATLSSDTAPSERERLSAMLSIEDALWQQGFSHI
                     AGVDEAGRGPLAGPIVAAAVILKSPVCGLNDSKRLTERKRAQLYEEIMRGGHIVSVST
                     IPSKQIDLFGIQAANQSAMRQAVTALSVSPDFVLVDGYKLSGFQQPAMQVIKGDARSL
                     SIAAASIVAKVTRDRFMLEIDECYPGYGFARHKGYGTRQHLEAIKRLGACPEHRQSFA
                     PFTDNASSEKTFLDFTTNPGRQDSE"
     gene            complement(52886..53836)
                     /locus_tag="PKN92_00890"
     CDS             complement(52886..53836)
                     /locus_tag="PKN92_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNZ47375.1"
                     /translation="MKALYFDGSLSIKEMPDPVPARGEALIQVLAAGICNTDLEITQG
                     YMGFNGILGHEFIGLVVECDNPFLRGKRVVGEINCPCRNCRYCRMEMTRHCVERTVLG
                     IQGRHGAFAEYLCLPEENLHLVPDSIQDETATFTEPLAAAFRIVEQLSPSSEDKILVL
                     GDGKLAQLITQTLWLYSKNTICLGKHPWKMELLRNFGITAVPFEKYKGESADIVVEAT
                     GKSDGLTHALQLIRPEGSVILKTTCYEGSPLSSVSAVVNEVKILGSRCGPFRPALEAL
                     ALGTVEVRPMISEAYALSDGVRAFSRAKCPDVMKVLLHMR"
     gene            54146..56002
                     /locus_tag="PKN92_00895"
     CDS             54146..56002
                     /locus_tag="PKN92_00895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ47376.1"
                     /translation="MSKTICPVCFTENAEEAERCHHCGALILSGSGTTPHELSSDAEN
                     SMDYKRGDLIAGRYEVHDLIGRGGMGAVYKVHDKVLDEEVALKVLLPEFVADKQVVER
                     FMNEVRISRKIAHPNIVRIHDIGHAEKTIFISMEYVDGSSLRSILDKRGKDSHMSIRQ
                     SIYIISQLCNALKYAHRYMVHRDIKPDNIMVTRNNHIKLMDFGISKLKDNRFESHAGV
                     IVGTPYYMAPEQIHNAPDVDGRADIYSLGVVLYELLTGTLPTGMRHSISQDYNHIPVE
                     LEVIIFRCLDPDREQRFQNPGDLRDALISLNDSLIDNSTPGIQSSHFSKADTGASNLT
                     TSILASAMESFLRDQGGDSASDAEKDSGLSSRIKTPAEESASGLPAYFETDSPRSPST
                     IRKKKAVEQSFFQQYKFLLIGFAVLVLVIALFVVRNRVWEISENLGIVAQETMQVDPG
                     EQAERLLAAADKFQVLIDVSNAYKKNKTPEGRALLEAVRDYFVETIEARAYARPFSMD
                     KLNEASLEAAQAAQIDGGSRMVEFADRMTREAVYFKFVLTRFDSTAGTAEFRLNNPYI
                     PEETATVAVGDLLLDRFLVVSITSQAVRLEDTDPKCAGRPLVARLMERVSDR"
     gene            56172..56780
                     /locus_tag="PKN92_00900"
     CDS             56172..56780
                     /locus_tag="PKN92_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47377.1"
                     /translation="MKLFLRMSMLVLLLIPLTSGCARDRNQSGISTETSGFSSAEEGA
                     KKELELLIKNHIQTSKTYNDAAQVPVVYRRPYYLKEYSVYFNGPDAFDIAYRELESKT
                     RPLMAEVTIEKVRFSTKMHRKKNLAREDHEFVRDTGVEKMVYELHNGRWTRIGAIFDA
                     KTTEEQVNGEWIPKREELERVLPEEQQPGWFGRLWNRIRGEE"
     gene            56802..58133
                     /locus_tag="PKN92_00905"
     CDS             56802..58133
                     /locus_tag="PKN92_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47378.1"
                     /translation="MTEVPGKDEAAIGVQGKNWLHEMPVVLALLLVLLPLFGGGTGIV
                     VGETVNSRLATVYALIHDGVWHIDRPLSEDPNPFENKTVDKVQRHDGHYLSSKPPVLP
                     LLMAGEYGALHRLFGWELTNTDQLRHVLRIMIFTLMKLPHIIGVLCFTLLLRLFLPNR
                     FHVAFLILALGLASPLTGFACQLNNHTPAAAGVMVCLYLGIGLYTARLLPTAWRFLLF
                     GFACAFVFVMDMPVTIFPAVLGLLLLQRFPKQSVLWAGAGALPLLLLHFALMVVITGN
                     PLPVQTQTSMYTFRNSYWRNPIGVDGLNEFWFVYLFHMTFGRFGVFLLFPITLLAFPG
                     VYKKIREPQNLLSLPYFALLTAFLVLSVYYVLFTNNYGGAAYGFRWYNGAIPVLLLLS
                     LPAVSAVRNWKCAFLLVPLFVVSAYSAWECFQAPWGASHEWTCRLLFGPVF"
     gene            complement(58130..59239)
                     /locus_tag="PKN92_00910"
     CDS             complement(58130..59239)
                     /locus_tag="PKN92_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47379.1"
                     /translation="MRPMPGKPLKPYTPLDHLCPFFTRFPLSFPLNILENTYPPKRVF
                     DPFCGCGTTLLAASVTGHFVSGMDIHPVACAVSRAKLASATAEEVIAKAEQILETTPV
                     NTQNKQNGFWNLCFHSSVYEGLLRLRERYLQGNLDQAEARLYGIVLGILHGPLQKGKY
                     LSNDMPATFAPPQDLLSAHWQARGSKPPPYSLQRLIAEKARQLPILDAASGSGVVQEN
                     SFLRAEAPAAENKFDLIITSPPYYGLPNKNCGLWLRSWLLGDDEASASQSQELHHQSL
                     TDYINDLGEVWKKAASFCRPGASLYLHFGKVHGSGAYSSLDVLHESVKRASTGWTVRS
                     IDAVKTAAKFMARRNFASPSPPPEDEWRIQMLLNN"
     gene            complement(59272..60762)
                     /locus_tag="PKN92_00915"
     CDS             complement(59272..60762)
                     /locus_tag="PKN92_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNZ47380.1"
                     /translation="MNYLALLKNMGLFCLVATGMFVPAVLCAFIYREDQMLPAFFFSM
                     MLTFSLGGLLYLVGKKSNDRLFHRETLALVGLGWLIGAVFGGLPYYFSGHLTFEDACF
                     ESMSGFTTTGATILVAIEGLPRSLLFWRDFTHWLGGLGIVLLLLIVLPFLGAGGKLLF
                     RSEVPGIVKSGLRPRIKDSALILLKLYCGMTVVMTLILCLLGMNLFDALTHTFGALST
                     GGFSPYQASVAHFNSFAIEMALVAFMLISATNFGTLHQIIHRQHKKAFASPEWRIFLL
                     IVTASTLLITVNLYQAELNGQTIIRPEHRGILPQGIELMRMALFQVASIITTTGFCTE
                     DFNLWPPLSQGILIAIMFIGGCSGSTAGGLKVIRIILFLKMAYWQIERSFKPKNVHSL
                     RVEETVIQPDMQQSIFAFFFIYMAIAALSTLYLSGLGLSLVTSFTAVAACLNNVGPGL
                     ELVGATGNYGFLPVSAKWLLFFLMGMGRLELYAILVLFVPSFWKHS"
     gene            complement(60918..62267)
                     /gene="trkA"
                     /locus_tag="PKN92_00920"
     CDS             complement(60918..62267)
                     /gene="trkA"
                     /locus_tag="PKN92_00920"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNZ47381.1"
                     /translation="MNIFILGGGRVGYHLALLLSEEVYEVTVIENDPERQEYIDQMLN
                     AQVILGDGSSALFLQSLDVGEADLFVACTGHDETNLLAAAAAKGLGARQAVARVEKAP
                     YMVESYMYDQLLNIDYILSPDALVAQEISNYILHPGVLVSEEFSHGHILLRQVQVSPD
                     APAAGHLLSEILKPGSGTLVGVIERQGKVFVPDGTTRILSGDKATLLSKREKLSDALS
                     KFQGRVSAIQRVAIMGAGTIGLWIAHTLESTVAEVKLFERNSSRALAVAGEFRKRNIQ
                     VINRDATTRDSMEQEHLETFDLFIATTEDDERNIVAGVLAREVGVSRVAAVIHHPDFA
                     PLAEKLGVTLAVTPRSVVANSVLKIVRQKTIAASTTLNEGEAEILEISISATSPLKNL
                     LLKDSKGKLPPGVLIATVMRGNEAFIPDGTTMIQEDDSLVLIATTENIHEIQKLFHR"
     gene            complement(62452..62625)
                     /locus_tag="PKN92_00925"
     CDS             complement(62452..62625)
                     /locus_tag="PKN92_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ47382.1"
                     /translation="MGVNVNVDTCDGCAECVDTCPTESIVIENEKAVVDNDTCIDCAA
                     CVDACPTDSLSME"
     gene            63152..>63374
                     /locus_tag="PKN92_00930"
     CDS             63152..>63374
                     /locus_tag="PKN92_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNZ47383.1"
                     /translation="MAIDLGTANTLVYVKGQGIVLSEPSVVAINKDTGKARAVGSEAK
                     SMIGRTPGNIIAVRPMKDGVIADFTVTEQM"
BASE COUNT        16135 a        15008 c        16583 g        15648 t
ORIGIN      
        1 atatcaatga tattaacact gccgcctggc ttctggataa tgtgccttta tatgtgcctt
       61 ttcagcgcat tgtccagaaa gaggataacc ggatcagtgc aaaaaaggcg ctgtctgcag
      121 cggaaatgaa tgtgcctcaa tatacacgga gcggctggca accgtctttt catgcgccag
      181 ggaagaaatc ttccgctgaa atgcagacaa atccggaaat tcccgcttct gcgccgacag
      241 tgcctgaaga agggaacgcc agcggatcag gcaaagtgaa tttgaataca gcttctctgg
      301 cagaattaca gacccttccc ggaattggcc ctaaaactgc cgaaaaaatt gacaattatc
      361 gccagagcca gcctttccgc tccattgaag aattgcagca ggtgcatggt atcgggccca
      421 aaaaactgga agcagttcgt ttgctgatta ctgtggattg agggcgctcc gagcagcttt
      481 ttgcttccgc cccggcaagc ggggtataat gctgcgcagg gagaggtgac cgagtggctt
      541 aaggtgcacg cttggaaagc gtgtgtgcgt ggaaacgtac cgtgggttcg aatcccaccc
      601 tctccgccag tatgtttttt gttcccgaag gtgtggaagt gcaggggagg ggcgaaagca
      661 cgcattctca atccttccaa cccgcagccc gtagatcgaa tccatgctta acggtgtccc
      721 ccgtatcctg atcattcgtt tcggcgcaat tggtgatgtg gtgcgcgtac tgcctgcttt
      781 gcatgcattg cgtgacgcat ttccacatgc ccagatagac tgggcagttg aacccaagtc
      841 cgcagatgtg attactgatc accccgctct ggatcagatt cacgtgtttg agcgttctgg
      901 cagtttctgg gcaggtataa aagcttttcg gggcttctgc cgccacctga gaaacagccg
      961 ctacgatatg gtattggatt ttcacggcat tctgaagagc gggcttgcca tgcgtgccac
     1021 ccgtgccgca tcacgtatcg ccttttccgc cccgcgcagt caggaattaa gccatctttt
     1081 tgccaaccga cgtgttacgc ttccaaaaca gatcctgaac cgcatcgaag aaaatttgat
     1141 tcttgccgca gcggcgggag catccgggcg cgggctcgaa gttactttgg ctgtttctga
     1201 tgaaattcag gagtcggtgg agtcctatgt gcacgacacc tttcaaagtg cgaaaaaact
     1261 cgtgattgtc catgcacctg tagaccgtcc ggaaaaacaa tggccgctca gttactacgc
     1321 cgctcttgtg gatatgctgt tgtctgacgg tcgctttgat gtacttttaa cctggggacc
     1381 cggccagcgg gatattgtgg aaaaaatcca cgcaaagtgc aaaagagacc ctgtcatagc
     1441 accggaaatg ccttccctga agtattttgc ctggctcatc cggcaggctg ctttgtattt
     1501 tggtggagat acgggcccca tgcatatcgc ttccgctatg gacacccctg tcgttgccgt
     1561 atttggcggt acatcgccgc agcgacacca tcccttccgt gagccctgtc gtgtccttta
     1621 cgcaggatcc ccggaccgca ctgaaaacct ttcacctcag gaagggcaga aactcctcga
     1681 aaagattacg tctgaagagg cttatgatgc ctgcacagcc attatcaaaa agagcgaagg
     1741 cggcggaaga ccggctattc ctgtttgagt atttcgaaac gttccagaat gggaaggtgc
     1801 tgagtgcatt gttcctcaca gaggcggcat tgggtgcagt cggcgagcag tgctttgatg
     1861 tccgggctgt tccagtgata ctgcatatgc cggcgggcgg catccatgcc tttgagcatt
     1921 ccgtagttgt aggattccat cagtcggaca acggggatgc cttccgggca gtcactgcag
     1981 tagccgcatt gggtgcacat gtctttatac gctgacgcca tcgctgtcga gatttcatga
     2041 atttcatcct gagacagcgt ctgcaggcgg ttcaccgtat cgataacctg atcaatatcc
     2101 tttttgttgc gaaagccgac cagtgcgaca gtaacctccg gaattgacaa gttaaatcgt
     2161 attgccgcat caaggatgga tcggtcgccg ggacgcaaga ctgcctgaaa gcggtcgcta
     2221 tgatccgtaa gaacgccacc gcccaatgta ttcattgtga cgacagccat atttttggcg
     2281 gctgccgccc gaacgccttc gaggcgcaga tggctgttct ggacgttcag accaataagc
     2341 atgctgtcaa aaagatcttc cccttcttca agcatggcgg caacgcgacc gtgttccaaa
     2401 tgcgtggata cacaaatatg gcggatgagc ccttcttctt tcagttgctg aaagattttc
     2461 agtacaccct ttttctttct ggcgggcaaa tcttcgggct tgacaagaca ccagatatgg
     2521 tagaaatcga tggtatcaat gccgaggcgc tccaatgacg cttcacattg ccggcgcacg
     2581 gcatcagggt cggcagccat ggttttggtg gagatgaaaa aaggttttcc ctgtttttta
     2641 atttccccga tggcgcgacc gaagatcagc tcgctctgaa aatccagata atccggagcc
     2701 gtatcaaagt agttgatgcc ctgttcaaaa gcatggagca ccacctctgc catgccgtcg
     2761 atgtcttccg gggattccag gcgcatcccg ccaaaaccga ttctggagac aagatgccct
     2821 gttcgaccaa attctttata ctgcattgag attgtagtct atcggtttgt atggcgtcgg
     2881 tcgacaaaaa tagtgctggt accgtcgatg ggctgtgctt tttgccttac ctgcgccagt
     2941 acttcaaaca ttttttttgc gctcttgaag acccggaatt tgttgtgggc taccccaata
     3001 gccaatgcca tgatgggcgc ctgcacttct ttgccgtccc gagtcattgc ggtaatgtaa
     3061 ccacgttcca gttcttcagg cttgtacagc tgcttgactt cttcatcgaa agtctgggca
     3121 agtaaccctg tgaaacgctc atagtcttcg agtttcagca tgataacgaa atgctgtcgc
     3181 cccatatggg caacggcggt ttcgtagaag ccctgagacc ggatcaaagc agtcagcagt
     3241 ccggctatag acttaagtgc ttgctcctgc agctcagcgc tccggcttcg gcaaaaggcc
     3301 tcaaaaccca ccacatccag gtagacgcag gcgatggcac tgtctcgcgc caactggtga
     3361 ttgagctctc ttttaaatgc ctctgtgccc atcatattgc taatgcgatt gacgtgaccc
     3421 agcgaagcga gcagggcatt gagagacgtg attttgtcca taagcctttc cagtttgaaa
     3481 ggctttacca gataatcatc ggcaccctgt tccataccat ggacaatctc aggctcttca
     3541 cccagcgccg tgagcataag tacggcactt ctgtaaagct cagggtcttt acggatgcgt
     3601 cggcagatct gataacctgt ctgacctggc aacatgatat cgagcaggat gacgtccggc
     3661 ttaagttgct tggcatactc aaaagccccg tcacctgtat taatggcaaa ggcctgatgt
     3721 ccttctttgg ttagctttgt gcgaaccagt tcagcaagat caacgtcatc atcaacaatc
     3781 agaattttac tcataaatag atttccttca tacaaaaaag gacataatac atgtcacggc
     3841 agacgccacc tgtcctcctc tgaaacgact tttttttcat acaaccagag caaacatgca
     3901 agaagtttta ttcttcctgt attttatcat aaaatgccac ggcataccat ctcactgcga
     3961 aaagaaaatt gttttgtttt taccattctt acgaatcaat cgcattttgg actgcttgaa
     4021 ccgaaagagg agttgaaaat tatgacaaat acagcacgtc ctttaaaggt aggacttata
     4081 ggatcaggcg caattgcccg ggaacagcac ctcccttatt ggcgcgaact ggaagaggag
     4141 gggcgtgttc ggatcgtcgg tgtatgtgac ctgatcaggg aacgccggga gaaggagtct
     4201 gccctttgtg caaatgccgt tccttatgca gattacaaaa aaatgctgca aaaagaatct
     4261 tttgacatta tcgatgtctg tacgcaaaac cgtgtgcatg ccccggcaac cattgcggca
     4321 ttaaatgccg gtgcccatgt acttgtggaa aaaccgatgg caatgagcag ccgggaatgc
     4381 gacgccatga tcgccgcttc taaaaaagcg gagcgaaaat tgatggtggc gcagcatatg
     4441 cgttttgaag cgcgggcgga aaaactgaaa gaagtggtgg acagcggagc tctgggcgag
     4501 atttacacag cggaaaccaa atggctcagg cgtcgtggta ttccgggttg gggtcagttt
     4561 catattaaaa aagaatccct cggcgggcct ctgattgaca tcggagtgca tatgatggat
     4621 ctttgtgtct ggttgatggg ctgcccgaaa cccgtggcag catcaggaaa agtgtaccga
     4681 aagttcggcg acagaaaaga tctttttaat gcggactggg gcgtgcatta tcctgcagaa
     4741 gaatttgatg tggaagacta cgccacagca ttgatccgtt ttgaaaaagg tattaccatg
     4801 cagatgtctg tttcatgggc agccaatgtt catgacgaaa ttgaagatat gtatattctg
     4861 ggcgataagg gcggcatagg cattaatcct ctggggtatt tcagtgccga tcattcgtca
     4921 ttgacccgta ccacctggga ctggctttcc gctgaagatg ggcaccgtcg tgaagtgagg
     4981 catttttgcg agtgcgtgga aaaagatctt cctgtaatgg tgaagccgga agagtcgctg
     5041 cgcatccaga aaattatcga tgccatttat gaatcttcac gacttaataa ggaagttatg
     5101 atcaaatagt atccggcgca caaagaaaaa agaagaaagc cggcagatca tttacggtct
     5161 gccggcttat tttttagggg gggctgttat ttcgctaggc gaaggaggtt gccctgtcct
     5221 gcaggcgtgc ggtcgcgata gctcatcaga tgcggaacat aacgcctttg cgcacggcgg
     5281 acatctcctt tgatttcccg gacttccagc attttcggct cgcccgccag ttcttgagca
     5341 gccgacagat ctcccgtatt tccttgctgg ttcagcgtca tttcgatgaa aagtgttgca
     5401 ttatcctcat aggtcctttt ctttttcaag ggacctttgg gcggaagtgg cgcatagacc
     5461 atgccccggg cggtatgatc aataaaaacg ccggtaccga cgacagcacc tttggttccc
     5521 tcgcagatgg acgcgggcgg atgcttgtaa attccccggt gcagtccatc atgggtgcgc
     5581 gagacaagct ttctgaaacc gagccacggc cactttacaa cacgcagcgc cggttcctcc
     5641 ggattgccga actgcccttc ataggctggc ggcacggcat gacttgaaag agaaatggca
     5701 agtaacacga gtaaaacagt tgcggtcagt gttttgctca tcctgaatct cccttgcgtt
     5761 tggacagggt ggcttgttta atgaaaccaa ctttaacgaa attaacttct ttcctttatt
     5821 ttacacaaaa tctcagtaaa tgtaaacatt ttactggcgg atgtctgtcg gagcttcttt
     5881 tggctgatca gcagcacttg aagcgggtgg catttgtcgc cgcagcaaaa cacgtcgccg
     5941 ctcccagaac tcgctgtcgc tgcctggtac agcgggtaca gcaaccgtgt ctgactcgcc
     6001 ctggtcactg tatattaaac gcaaccgggc gagatctgct tcggcaaggg agcagaaccg
     6061 gctgcctggg cattctgcgg caatccgctg aaaaagactc atcgttttca gcggctgatt
     6121 taaaaaggac tcatatagct tgccgagact gtaggctgct tccgaccaga aatccgggtc
     6181 tttcaccgga agctcaatca gttcttctaa aagggaagcg gctttttcat aattcccctc
     6241 ttcacgaaac cagtgggcgg tgaattccag tgcccgggat ctgtcctgaa ataattcagt
     6301 caaaacgtcc tgagcttcgg taatttttcc tgcctgcagc agcaggcgcg tctgaattat
     6361 agcaggtgct tccggtattt tggtgccgac aggtgttaat cgttgaacgc cacgccagct
     6421 gagagcattg accagtgtca aagaatactg gacactcacc ggaatgataa acaggatcag
     6481 ccacataaga agaggagccc gttcatcttt cgggctgccc cagtaaagaa cagtaccgac
     6541 aaggaaatag atcgagaaaa ccataaacat cagtgtcagt gcgaccttga cgggcagtgc
     6601 ttcctgattt tcaagccatt tctttttgat gaaaaaactg atgccggtca gttcgaggag
     6661 aacagcgatt aaaatagcaa ttttccacca tgcaggcagg tacacaatgg ctgtggcagg
     6721 tatttcagca gattgtgata cggcaataaa aggcatgaaa gaaaaatgct ttctgacggg
     6781 acttgttcgc gtctcatcgg aatatgtgtt gttttcgtag agaaaagagc cgttatgcgc
     6841 tgaaatacag atttgtgcaa cagacggcca caaccgaagt atacctgttg ccggcgcaca
     6901 gttcaattgg aaagcaaagt tccgcttttt tgctttttat cggctttgcc atgtatcctt
     6961 tacaggaata aaaaatagtt aagagttgca gtttttctgc ccgttgatgc agaaaaagga
     7021 ttgttttctt tggcgttgag gtgtgggaag tgcgccactg cctctgaaac tggcggagag
     7081 ggagagcgaa agagtcacat cgatgaaaga acgcattgcc ttgaacctga ataccatcgg
     7141 gtgtcggata aaagaagcag cggaacgggt gaaccgttct gcggaagacg tgactctcgt
     7201 tgcggtaacg aaaagtgtgg gtcttgacga gatcctggct ttatatgaat tgggcatccg
     7261 tgattttggt gaaaatcgtc tggacgttgc attggaaaaa ataaagcagt gcccgtcaga
     7321 tatccgatgg cactatctcg caccggtgca gacccgaaag gcacgggata ttgccgcccg
     7381 ttttacggtt gctgatgctg ttgacagagt gcgggcggct gaagtgctgc agaagcgatg
     7441 cgaagaattg gactgccact tggaaatttt agtggaagta aatgtgtccg gtgaagaaag
     7501 caagcatggc tttgacgaat cacttctctc tgaagctgtg gaaaggattc gtcgaatgga
     7561 tcgcctgtct ttgcaggggc ttatgactat ggcaccctgg gaggctgaag aaaaagtgct
     7621 gcgccgatgc tttgccggtt taaaaaaact gtgtgttgct cataatctgc ctgtatgcag
     7681 catgggcatg accgatgatt ttgaaatcgc cattgaagaa ggctccacac aggttcgtat
     7741 tggcagagcg ttgtttgtgt aaaaaaagga ttgttctgca gcaacagggt aaaatgctgt
     7801 caggcaactg taataaggta cttctgtatg aaagaagaat tgaagcaatt ggaactggat
     7861 ggaagcgcac gtattgcgga agcatcggat ctcaaatcac tggaggaggt gcgtgttgcc
     7921 tatctcggga gaaaagggcg tattacagag atattgaagg ggctgggcag tgcgtctccg
     7981 gaagacagac ggtcgctcgg gcagatggcg aaccttctga aggatcagtt tgcgcagcgt
     8041 attgatgcgc gtaaaacaga actggaagca gcgcttcgtg agcaaaaagc acaggaacaa
     8101 cgggtcgata tgagtctgcc cggcagggcg attcctccgg gacacgtcca tgtgctcaat
     8161 caaattgccg atgaaatttc ggatattttt attcaaatgg gctttcagct cgcattagga
     8221 cccgacgttg aaacggaata ttacaacttt gatgccttga atacacccga tgaccatccg
     8281 gcgcgggatt ctcatgatac tttttttgtc aaaccgggtg tggttttgag aacacagacg
     8341 tcacctgtac agatcagggt aatggagcag acccgcccgc ccgtggcggt tatagcaccc
     8401 ggtcgggtct atcgtgtgga tctggatgct acgcacagcc ccatgttcgt gcagatggaa
     8461 gggctgctgg tcgataaagg cattaccttt tctgatttaa aaggaacgct gatgcacttt
     8521 atacatagtt ttttcggccc tgatatacag gtccggtttc gacctcactt tttccccttt
     8581 accgagccta gtgcggaagt ggatattctg tggacagcac gggatcgtca tacgggagaa
     8641 gtaaaaagca aatggcttga gatcctcgga tgcgggatgg tgcatcctga agtctttaag
     8701 aatgtttcct acgattatga agagtactcg ggatatgctt tcggcctggg tattgacagg
     8761 attgccatgg tgcgccatgg tatcagcagc attactcacc tgtatgaaaa cgatatgaga
     8821 tttctggagc agttttaata tgcaaatttc actgaactgg ctgaaagagc tactgccgct
     8881 tgaagcggat gctgatgaga ttgccgaaca ccttacgatg cttggactcg aaatagaggc
     8941 ggtgcgccgc ccggggcaag acatagaaaa tgtttttgtc gggcgcattc tggatattca
     9001 accgcaccct gaagcggaca aactggttgt ttgccgtacc gatgcgggac agggtgaagc
     9061 gctgcaaatc atctgcggtg cgaccaatat gaaggttgga gataaagtgc ccaccgctat
     9121 cgatggtgcc acgctgccgg gcgggtttaa aattggcagc cgaaaaatga ggggaattca
     9181 atcctgcggt atgatgtgct ccgcccgtga actgggactg ggcgaggagc acgccgggct
     9241 tctgattctg gataaaaatg cgcccatcgg tcaggatata cgggactttc tcggactgaa
     9301 tgatgtggta tttgaaatag aagtcactcc caaccggggg gactgggcaa gcatgattgg
     9361 tgtggcgcgg gaacttgctg ctttttacgg acttcagatg acgctgcccg atgccgaagt
     9421 caaagaatca ggagagcctg ctgctgcctg gtcctccatt actatcgagg caccagacct
     9481 ctgtccgcgc tatgcggggc gcatactgcg caatgttgtc ataaaaccct cgccgccctg
     9541 gctgtgtgcc cggctgattg ccgccggaca acggcccatc aacaatgtgg tggatattac
     9601 gaactatatt ctcatggaaa cagggcatcc gctccatgct ttcgattatg aaaaactgaa
     9661 agagaaccgc atagttgtcc gccgcgcccg cgccgatgaa aaaataacca ctctcgacgg
     9721 aactgagcga cagcttcagg aggacatgct ggtcattgcc gatgcagaaa atgcacaggc
     9781 agtagccgga attatgggcg gtgccgacag tgaggtggaa aagactacaa caacgatcct
     9841 tcttgagagt gcctggtttg aatctcgatg tgtccgcagc acttcacgcc gtctgtcttt
     9901 atcgacggaa gcgtctcagc gattccagcg tggcgccgac atgcagatgc ttccttatgc
     9961 gctcaacagg gctgcacggc tcatgcagga attggcagat gcggaagtgg cacccggtat
    10021 tttggatgcc tggccccaac aaagagcgta tcctgccgtt acgcttcgat acgagcgtac
    10081 tgtgtcccgt gtcggtgtga acgtggcgcc ggcagaacag aagcgttttc ttgtgagcct
    10141 gggctgtgaa cttgagtccg aaacggacgc atctgccact tttaaaattc cttcctggcg
    10201 gcatgacatc aggcatgaag ctgatttaat tgaagaggtg gcacgcctct tcggatatga
    10261 aaatatcccc gtcaccatgc ctccggttcg ccaggccgaa agtgtttttg ctccccatgc
    10321 gactattttg cgcaatttca gacggtatct ggcggggcaa gggcttacgg aagtgatgaa
    10381 ttggtcattc ggcagccccg aaggtgctgt tgatgccggt ctacctgtat caccggcaga
    10441 tcaggtctat ctgtccaacc ccttatctga aaattatgct gtcatgcgtt tttctctgct
    10501 gcccgggctt ttcgggacgg cggcatataa cgctaaacga ggaaggcgtg atctgaggat
    10561 tttcgaactg gggcctgtgt acagaaatag cgcagacgaa attctgcccg ctgagcctat
    10621 gtacgcaggg attcttttga gcggcaatgc gcccgggcac tggagccgtg cggagtgctc
    10681 ttatgatttc tacgatatta aaggctgtct ggacgtaata gcccgttttt tcgggaaaga
    10741 gttgacgctg gaagaaagtg cgtcttccgg ttttcagaaa gggcagttcg cagaaataaa
    10801 atggcgcaaa cacattttag gatgcatcgg gaaggtggca gatgaagttg taaaaacctt
    10861 tggcttgccg gaaaacacct atgtgttgga aattaacttg gaaatcctcc tcgcgaaaaa
    10921 gacggagccg gcgcaattta ctgctatccc gcagtatccg gcgtctcttc gggatcttgc
    10981 cgtggttgtc gatcgggctg ttcccgcaaa agatctggtg gcggcgacga aacaggcagc
    11041 cggacctttg ctcacggctg tgaatgtttt cgacgtttac agcggaaagc ccctgccttc
    11101 agataaaaaa agcattgcct tgagcctgct ttttcagtcg cccgaccgaa ccctcaccga
    11161 caaagacacc caaaaaacta tggaccggat tgtgtcgcgg ctccggaaag attttgaggc
    11221 tgaacttcgt tgaaccaatt tcttcagaac tatattgagc agataaatgt gctggccgat
    11281 cagttggaaa agaccctgac cagttacggc actcagctcg ccagcagccg gataaagaat
    11341 gaagaagacc agaaagaaaa atggatgatg gcaaggaaaa tcaaaacaat ggaagaaaat
    11401 cttgataatc ttgccgaact gaaacaggag aatgaagagc tgcggcagaa aaacatgcag
    11461 gctgcagagc atgccaaacg aattctgatc ttaacgaaaa gtctggcgga tgcattgaga
    11521 ttatgagtat tctgaatcca aaacagcagg tggagattgc aggcaataga ttgcaggtcc
    11581 ctctttacat tgatgaagaa acgacagagc agcttgccgc tgagataagt gccagactga
    11641 agaaaatcgg ggcgacgtat gaaacagtga atacgcagcg caatgccctg gcgaccgcat
    11701 atgactatgc tgtagagaca gctgagacaa aaaaacagta tgagcaggat gttcgagaaa
    11761 tactgacggc acttgaaagg atcacctcct cgttgaaaga actgcttgac cgtttcccgc
    11821 cggtgtgata tcgccggagc aggaatgaaa atcaagggct caggggaagg ggattctgtg
    11881 cagaggaaag aggtggtccc cggcttctct tgtcaacacc cgggagcctg aacgaaagag
    11941 gcgctgcctc caccagtcgt gggaaagagg gcggaaatca ggctttcctt tttcatcgaa
    12001 gcctgtaatg gatgcaactg ccagattcgg cgcgtacacc aacacttccg cccgcagcgg
    12061 tggcattaca gaaaaagaag ccggggaata aacaggaagg gcggacgtat ttctgtcaat
    12121 cgggcgcagc gtttccgaga gaaaataggg gacaaagccg acgtagtcga tatcggggaa
    12181 aacggcaaga tactgaccgg ggtgctcttc tgcgctgatc ctggctgctg tgcgaaaacg
    12241 tgctatgtcc gcccctgcac ggtaccactg taagttaacc cagatctgac acagcatggc
    12301 agcacagcag accagagttg acaaacgtac gacagaagtg ttccagtgcg gtatttcaga
    12361 aagagccaga aaaaaaccgg cggcgccgac ggcagcaagt gctgtcggaa tcagcagcgt
    12421 agaactgttt tctaaggtaa ctaaatcgat gggcagcgag ccctgaccga tcctgaaggc
    12481 aaaagcgccc agaattccga aaataaatgc aggatgcctg taccgtcgca tgaaaaaaca
    12541 aagcagtgtc aaacctgcag caaggccaat ataaaaagcg atggtgtaat aagaaaaaaa
    12601 agaaaatgta gatgaaagga agccgatggg attcaggaga agaagcaggg gaataaacat
    12661 agcatgcaaa gaaagggcat gggggagaaa aatctgtccg ctaaaaaaca atagtagaat
    12721 gcctgccgct gcaatataaa tatggtggcg ggtaacggat cgttctgctt ttttaaagca
    12781 aatttcctct agcacaaaaa tgacggcaaa gagcggaata ttggaaggcg taaatacgga
    12841 tacgagcaga taggtcaaaa aaggaagcca cttgtgcccg atcatggacc ccgatctgca
    12901 cagtgcgtaa aggagaagaa ctgccaaacc caagagaccg ggcaaaagcc attgaatgcc
    12961 gcctatcgac aaaagggctg tgctttttat gggatttgcc ataaagagta cggcagagac
    13021 ggaaccaagc caccaggggc ctcgcgtaat aaggcgggtt aaaaagaaaa taagaaccat
    13081 gcagccgtag agcagcagaa aatttacaac aagatatccg ggtatcgagt cgccaaacca
    13141 tcggtgcatc tgctgaaaga taaggcgggg gagcaaatgt atttcgtcgg gtgtcgccgc
    13201 ctgccggtac gacaatccga ccggttgcat ggtgacaagc catccataaa ctataaggag
    13261 gtatgcagcg tcggcaatta aaaagggaag gatccgtcca ttttcgtttg aggcggtttt
    13321 agagaaattt ttttttccag tggcaggcat ggtctgttat cttttccggg tcttaaaaat
    13381 cagcatagca tagctgcatt tatatcacca atttttcgtc tgtgaaaagt attgttaggc
    13441 atggcgtttt ccgtgcgaac taatcttgga aagttcttga caaagccttg gggtgagagt
    13501 ataataactc ttgacacttt cggaatttat gctattagta aagaatagag agtttatgat
    13561 gtgtgatgct tatcaggaac aagtttcacc atctcacatt taagtgttga ctgaaatgta
    13621 atttctggaa gtcatgatag atcgcaacag agaagtcggg catatttatt atgatctgag
    13681 tccctggttc tgcatccgga gatgataaaa attttgtcca tctgtgatac cggaaaacgt
    13741 atacaggagg taccggcatt tgggcttcag aaaaccaaga aaagtcatcg gcgttgacat
    13801 cggcacgcat gcagtaaaat ctgttttgat gactcgcatt tcgggccgtc tcaagataga
    13861 gcaggctgca atgtgtccta ttgaccgcaa tctcttaaat cttgacccgg tcaaagcgca
    13921 ggaagaggct gttcgcagaa cacttgaaag aatgccggtt cagcaaagtc tcatcgtcgg
    13981 cgccctcgcc ggtcagactg ttgttgttcg gtatccgcgt ttgaccgatg tgtcgaaaga
    14041 acggcttcag ggagcggtag cgaaagaggc aaccaacagc attccctacg atctgaaaga
    14101 tgtttttctg gattgggtcg tgctggagga atactcggag gaaggccgcc atattaccag
    14161 agtgctcctc gtggcggcgc gtaaagaagt ggttgacgtg cgccttcagg tgatgcgtgc
    14221 ggcggggttg gagtgcggtg ttttgggggt tgactccctg gcgcttgccg atgccgcgga
    14281 agcatgcgac tttttccaga agggcgaaac agttgcgctg attaatattg gactgaactc
    14341 gtctagcatt caatttgtca aaaacggtgt ttccaatttc attcgtgacg tgaactgggg
    14401 agcccgggaa ctgattcaat ctgttgccaa gggcatgcgc tctaactacg agcaggcact
    14461 gtcaaccatt gaaaattatg ccatcgacca tgtgtctgtt cctgaagcac aggaagcagg
    14521 cggtgcggaa agcagttttg cttacatgga aacgcctttc cttgaagata ttccgcaggc
    14581 agaagcatac atgggtggtc atgacgacga acaaggatat atggagtctg aattgagtta
    14641 catggacagt ggctacggta aatcggcgac actgtccgac ctggtagcct cgcccttgaa
    14701 cagactgggc gtcgaactgc gccgttcctt tgactattac gagcatcagt tgtatgagca
    14761 gcctgtagac cgtatcttaa tcagcggtgg tccggtcgct tttgcgcctt tggttgaaag
    14821 tctacaggaa gtgcttaatt tcagtgatat agaaattgtt aatccggcgg caagtgccct
    14881 tctagtttca gaagaggctt ctctaagtgc ttttcatgcc caccccgcac aatttgctgt
    14941 cgctgtcggt ctggcagcac gcggaatggc ggaactataa aggtcacagc agcatgataa
    15001 aaataaattt gttgccaaaa gagctgaggc cggtacaacg gacgccgatc ccccatatag
    15061 tcagtctgct gattctggcg ggtgcgctgg tctttatgta caacattttt attggtcagc
    15121 gtacggagtt gcttatcgtt gacggcaaag ttcaaaagat gacaaaagaa cttgacgggc
    15181 tgcaggaggc tgtggaagag cataaagaac tgattaagca gaaatctttg ctccagagga
    15241 aaatccttgc aattcaggag atcctggaag gcaaaacagt gtggtctgaa caattgcacc
    15301 ggctggcaac actgacgcct gaaaacatct ggtacaagcg gatacgcatt acttccaaaa
    15361 agtttgctga agaaaagccg acccttgata agaagacagg tgcaccggtt gtggatgcca
    15421 agacaggagc ggttcggatg acccgtgttt ccgtggataa acagatcctg gaagtgtccg
    15481 gatatgcaat tgatgacgag cacgggttaa gcagtacctc cacgcttgcg gcacgcacca
    15541 ccaatgacaa agatttttca aattatttta tgctgtacaa ttcaaaaatc gtggataccg
    15601 attttaacgg acatcccgtc cgcgaattta cttttgaata tataattgga ggttgaccct
    15661 gttacgcact gacgaggcgc tttaagcagc cacaatcaca aaacaggaat tacatatggc
    15721 agtactagca aaaggaaaga ttacccggca ggattgggca ttcgtgggta tcaccctcgg
    15781 tgtaacagcc ctgctttttg ttgcctttta tcttttcggg tatacaaagc tccaaaatag
    15841 aatcaaggca caggaagctg aagctgtgcg gatcagtaaa gaactgaaag aagcgcacgc
    15901 tcttacggat gggattgagg atctccgtaa agaggcgcag gaaatgaacc atctggttga
    15961 gtcttttgaa gacagactgc ctgaagaacg caagatcccg gcacttttgg cgcgctttga
    16021 aaaacttggt tatgagatgg ggctgcgcgt acaattgtct tctttgccta cccggaagac
    16081 cttaaataat gatatggaag tgatcccgta caaagccatt acggacggac agtttcatca
    16141 gatcgtttct tttattaata tgcttgagag agacgagcgt tatctcaaaa tttccgactt
    16201 ggatattgac gaagaaattg aaggcatttc caaggcaact tttgttctca gcactttcag
    16261 attcctgaat gaaccggaaa tcaaagaatg aatgaacaaa ataaaaaaca gatcattatg
    16321 gctgccgtcc tcggggtcgt gctgataggt gttcttgttt accaattcgg tctcagcggc
    16381 tccagcagcg gtggcaggtc tgcagacacc aaaacagccg agaaatcaaa aacgaccagc
    16441 aagaaaagtt cctcaaatgc aaagggagca ccagtagccg atgcggctgc tcttgcctca
    16501 attgatattg ccgcgttgat tgccacggtt gaggtggtac cttttgatta tgctttgtcc
    16561 aggacggcga gagatcccat ggcaccattg gtcgggaatc ttcctcccgg actggcggca
    16621 gaatcagcag ccgctgatga gacggacgca ctggcatatg tacaacgggg gaccacggca
    16681 acgcgcgtcg taagtggaat tatttgggat aaacagtttc ccgttgctat tgtggatgat
    16741 attgttgttc atgtcggcta tgaatttcct aatggtgcag ttgtgcattc tattgagcca
    16801 ttacgtgttt tgttcaaggt tggcgaagcc ataatccctg tggaaatgaa ggagttctga
    16861 tttatgcgtt accaccggca tgttaaggcg gtaggcctaa gtgtggccgt tctgttgttg
    16921 gcggcagttg cttgggcgga aatggaactt caggcaaagc ccagtgcgtt attggaaagc
    16981 agcgcgcccc ttgcacagca ggtgattcag ttgcctgcca ataattcacc gggaactgct
    17041 catgcgcaga aggcagatat gctgaaatct atgctggttg actttgtcaa tgagcaggaa
    17101 atgacagcaa ctaaagaccc ggaagccgtg tctttgcaga ttattgacaa ggacttgggg
    17161 ccagtgtccg gcatggaaca gaaggttctg gcagtgcttc agatgaacga tccgtccgaa
    17221 gtggccgata cctatgaggc agcggacggc gttgacaatc agaatgcggc ggcacccggt
    17281 gacgatgcgg ctgccgacgc gcctgacctt ccggaaagag agccagcacc taaggtggat
    17341 gcggcacagg aagtagcaga ggaagaggaa ggtccgcttc cgcctgcccc gcgtgtgcag
    17401 acgtctcagg atgtaaaaga tgccaatgct tccctggttt cgcaactgag gactttatct
    17461 gacggctcgg aaacgccggg cggtggatca gtgacggcag gggcgccctc cggttccagc
    17521 gatacggctt tgttttctaa agacggatca gatcctgcgc aggcgccgaa ccgtgcaccg
    17581 aaatcaggct cattgaacga tctggttaat ctggatttca gaaataccga tttaagccat
    17641 gtggttgcca ttcttgcact taaagcgaac attaatatta ttgccggcgc agatctgaaa
    17701 ggttctgtga cagcgaatct ttatcaggtt ccgcttcatg tagccatgga aactgttttg
    17761 cgcatgaatg gtctgggcct gatcgaagag gacggaattt tctttgtcgt gccttatgaa
    17821 gaagcagcat cggttaaccg gaaaacagtc atggttgcca tggacaacgc caatgttgac
    17881 gatgttcgga aggtactcac ggacgtgacc aaaggtgccc gcgaagaagc gcaaatcagc
    17941 atatcttcaa acaaggcaac caacgttctt attatcagcg caccaaaagc cagagtcgac
    18001 gaactcgctg ccatggctca tcagcttgac gtggctgagc ctgttcttcc cactgaaaca
    18061 cgtgccatca gtcttaatta ttgcgaaccc cagaagttgg tgcctgtcat tgataaaatg
    18121 ctgagccccc gtatagggaa ggcgtctgct gatgagcgtg cacgccatct gattatcacg
    18181 gatcttcctg tggtggtaga gcagatttcg gatctgatta aaaccctgga tattcccacc
    18241 aagcaggttt taattgactc catggttgtt gaagcgctgc tttctgatga tgccgacaca
    18301 ggtgtaaaat ggctgatgaa ttctcttcat cgtatgtcca gacgccaggc attgctggga
    18361 gaagaaggta agagcttagg aaatattcag gaacttgctc ttggtgcgga ccttgaggca
    18421 ctgcggcagc ctgcaggaat gcttaccttc agccttctgg gtgatcatat cgactggagc
    18481 gggcttattc aggcggaggt tcgtaacaga aatggtcgcc tgctctccaa tcctatgctg
    18541 cttacagtag aaaatgaatc cgctaatatt tccatcgctc aggaaatccc ctatattgag
    18601 ttgtcgcaga caaatgcggg cggttcccaa accaatacgc gctttaaaga aattggtaca
    18661 gttctgacag ttaccccacg agtaacgcat gataatacga tcatttgcaa gctggaaggc
    18721 aaagaaagtt atgtttccgg cgaatttcag ggtgtcccta ttgaagacaa gcgtgaagtt
    18781 gcatcaacca tgcgcatgca gacaggccag acgattttta tcggtgggct gaggaagtct
    18841 caaggcacat caacagctaa gaaaattccg gtgctgggtg atttgcctat tattaatttt
    18901 gctttccgtt ccaatagccg aaccgaacag attaacgatc ttcttgtgtt ccttacatgc
    18961 tcagttgtga atgaacaaga ttaccagtta agtccgcatc agcagcaggt cctggaagat
    19021 gtgccgccca aagagatgaa agcagacgcc tgggaaacca ccctttatga cacacgtcat
    19081 ccccaggcgg taaaagaact tcagtggaag tggcgtcggg aaccgtaatt ctcttcctta
    19141 aaataaaaaa ctacagcccg aaggtcctat ctggtcttcg ggctttttta ttactgtgag
    19201 agaggaacgt atgccgtaga aaaggagacg ctctgcgccc ttcaacggct ttcttttgac
    19261 gcgtggataa cctcagtctc ggtaaccaga taatgcactg tttcgtcaaa attatcgaag
    19321 gggagcgagt ctgctatttg taaatcaaat cccaatgcta cccgaacgcc cttgtggctt
    19381 tttaaaaatc gatcataata cccgccgcca tatcccaaac gatgcccttc ccgtgtgaac
    19441 gcaattcccg ggatgataca gagggagcct ggcgggggcg tgcgcaaagc cactgttccg
    19501 ggagcaggct ctagcaagcc gaaagcgctt ctaacgagtt cagcccccgg ctgcatctca
    19561 gcccagcgca tagtgcccgg gaggcagtgg gcggtatagg gaaccagaac acgttttccg
    19621 ctctttgcgg caatggcaag aagtgcatgt gtgtcaactt cattgtcctt agatgagatg
    19681 tagcacagca cggcattact ttcctgatag aaggaagaat tgatgatacg ctggaagatg
    19741 cttccgcttt ttgcctgcca gtcttcggaa gaaagggatc tccttttttc cagataaaaa
    19801 agccgtgctt ctgactttga cgaaaaaggc tctgacatgg attctgtagt gtgaccctat
    19861 gcaaaaaaag atttacaaca gagagcagaa aggctgcgtg ggtttatttt aaccgaattg
    19921 acgccgtaac agaaatagag gagcacagtc tttttgcgtg ccctttatct taaccaatgc
    19981 tgttgttttt cttctcgaat agtcaatgat ctgcagactt cgcaaggaac ctatttagaa
    20041 aaccgcaaag agtcgtataa aaaacggcat gaaaataaat taaagagaat gaatctgaga
    20101 gtacatcggt aatactgtag ttgcgataat gacagtcgac aatgcaggat ttaaaaagcc
    20161 tcctgccgca tctgtacggc aggaggcatg ttctttttac acaggtacta ttttggtcgg
    20221 gcgctcaaga agcggtctct gttgcttcca aagatgaggg ggcttcttcc tttgctgcta
    20281 tttcttttgc ctgcagcgcg atttcaccgc ggcgcagata atccattgct ttaaaaagaa
    20341 gacgggctga ttcctgggga gcatggaatc cgtgagagtt ttcagactgt acaatgtcga
    20401 taaggaagct ggcaccgcgc tggcactgat aggcctcatg caattgcgct tcagccagac
    20461 caccatcttt tgcttctacg agatcgtcga tgaaggtggc aagcccctca agagccatat
    20521 tgcacatttc cacagtgcgt tcctgaatgc ggtttacccg ctgcaggagt tcttcttccg
    20581 atactttatg gcatgtctgg caggagttat tgatgttcaa cagcgggctg cgtacatggt
    20641 gatcactgat cttcaggccg ccctgacgca tgtaaggcat atggcaatct gcacaagcga
    20701 cccctgctgc cgaatgggtg ccctggctcc acatttcaaa ttccgggtgc tgtgccttga
    20761 gcatctgggc gccggtttct ttgtgtgtcc agtctgtaaa gccgatttca ctgtaatagt
    20821 cgagaatctg atctgcccgg cggcctttat accaggggaa ggtgaggcgg cggctttcat
    20881 ccggagcaaa atagtattcg acgtggcatt gggcacatac aaaagtgcgc atttcctggc
    20941 gcgttgccat ggtgttcacg tcataatcgg caataccttc ttccgcaagt tttgcggcac
    21001 ggatgccggt catgaaagac gggcgggtaa tgcgaagctg catggtttcc gggtcgtggc
    21061 aatcaataca ggccacagga tgttctacca gttttcgggc atcctgatac ttcatggggt
    21121 tgattttttc aaaaccggcc tgaagatcgc cattgccaat ctctttatac actgtataca
    21181 tggaggcatg gcagttcatg cagtttccgt actgctgcac tttctggcgc tgcgtgaagg
    21241 tctggtcatc aagcatatag gcatgaccgc gcttttccct gtagtcgatg gaaaaggcat
    21301 agcctgccca aaggcgtttg agaagaggga ttgtttccag tttgctcttg gctgtcactg
    21361 tgcggggatc ttcatcggta ggagtgtggg gtaccgcttc actgccgccg aaattagtgc
    21421 gctccatatc aacagtctgc ttatactgat catactgcat ggggaaattc atgccccatt
    21481 gcgcaggatc ggttgtttca tcggtcactt caacgacacg catgaagggg ttcttcgctt
    21541 cctgctggcg ttggaagatg ttgatgagga gcccggtgac tcccagagtc gcccctgcag
    21601 ccagtgcacc ggtaacaata ataacaatca gaatacgccg ccacggcaat gctgcggaat
    21661 aatcggacct gttgctggag ctcataacgc tgtgattcct ttcccgataa aaactacata
    21721 aaccaatgct actattttaa atgacccaca gaagtgtggc atctaatgca gtccaaagcc
    21781 tgaggcccgt gtccgaaacg atccacttcc tcaacgattt tgctgtgaca ataacggcat
    21841 gcgccctcgg tcacctgacg gttccctggt ctgatttgaa tgttgtcggg gaagaacccg
    21901 gtggtaaaag cataggaatg atgcatgcca ttcatcgctt ttacccagta ttttccaaaa
    21961 aaattatggg gagtatggca gtcattacag acggcaacag ccttgtgact ggatttaacc
    22021 caaccgtcgt agtactcctt catcacatgg caattggcgc atgtttccgc atcatcgttc
    22081 atgtaggata agcctttggc ataaaagaac gtgtaagttc ctactccgag accaagaccc
    22141 gaagaaatgc cgaaaaacag ggatgcgtaa aaaagccgcc tcagccaggt atgttgaaga
    22201 aagtcagcca cggtgtccgt ctccactcag acttaagtta ttattacctt acagataata
    22261 tataaaatgg aataaacaaa gcaaatctgt tgttaagagt aaaaatgtat atgcggtgta
    22321 taaaatatgc agttgaaacg atatgttttt atccaagcat aacgatcatc ttatttgctt
    22381 tgcgggcggg gaaagcattc attccagcaa ataatagcgt attaactttt ctcgcgaaac
    22441 gaacggttac ggcgcatacg caattgcccg catgccgcat caatgtcgcc ccctctttcc
    22501 tggcgcaagg tcgctgtaat tccggcatgt tccagcacgc tcataaacct gtcctgcctt
    22561 ttttggggag agggagtaaa cggcaacccg gataccgggt tccacggaat tacattcacg
    22621 accgcatcca acccttttag aaatgaaacc aggcggagcg cctcttccgg agagtcattc
    22681 gtattctcca aaagtgtcca ttcaatggtg atctgccttc cccgacggtt ctggtaccgc
    22741 tgcaaagcat cgtgcaaatc ttgaagcggg tattttttat ttaaaggaac cagtgcggaa
    22801 cgcagatcat catgtgcggc atgaagagaa acgcttagtc gcagttggcg gggttcttca
    22861 gccagtcggt ctattcccgg tatatcgcct gcggtggaaa tagtgatttt ccgggctccg
    22921 atacccatcc ccagcggatt catcagcagg gcgatacttt tcattacggc atcatagttt
    22981 tgaaagggtt cgcccatgcc catgtacaca agattaggtg tgctgttcga ctgcaaattt
    23041 cctgcacagg cgagatgaag cgcctgctca accatttctg cagcggagag gctgcgccgg
    23101 aatccggact ggcctgtggc gcaaaacgag caatttaacg cacatccggc ctgcgatgac
    23161 aggcagaagg tcagtcggtc tttgtgcctg agcagcacag attcaatgag ctcgccgtcc
    23221 ccacattcca gcagaagttt gcaggttccg ttttgctttg actgcttttt ttctatcact
    23281 ttcagcgcaa aaagagtttc ctgagcattg agacggtttc ttaactggag gggaagatca
    23341 gtcatggcgc tgcagttaaa aacacgcttc cgatgaatcc aggtgaaaat ctggcgcccc
    23401 agacggggtg gctccgaaag cagttctgcc atttcttcag gtgtggagcc tgtcagatga
    23461 aaggcttcga aagcggcggt catggcaccg gatcctggtt ttcttccgcc gtacctgcag
    23521 aaagatcgtc aagggaagta atgtcgcttc ggaacagatc cacctgctta tgttttttgt
    23581 atataaggtc cgacagcttg gcagggatat caaagagtgc ggggtcaatc tcgccgttgg
    23641 tttcaacttt tctcatgatg aagcgcatcc acacatcttc ctgcgggaaa gtgcactcta
    23701 tctgagtagg atagtgtagg tcattgttgt tgtaatagtg gcttttgttt gttcgggcga
    23761 cgggaacacc tgattcatta tagagaatat tctccagcac gacccagggc gcaccctgca
    23821 gtttaagtgt tcgtttatgc cttaaggcaa cgccgcttcc ttcccagagt acaagggtgg
    23881 ctgtttgctt ttcttcgtca tatgcggtca tttgaatcag gcgtggattc aattgtttcc
    23941 agtcttcagg ctgaaacatt tcccgcacaa tatcagcagt ggtaacggaa cccaggcttt
    24001 cttccgatgg gcggtagtaa tattcgcgcc gcgtgggaaa ttctatgagg aaagagtctt
    24061 tatcataaat gaggttgagt atacgggttc cgtaccttct tccaaggaca tagagggaat
    24121 ccggggcggc gaaagacaca acgctttccc gggaaacctg tatcccctcc atttccggga
    24181 gtttaaccat aatggttccg gtcgctttga aattatgaag ccgggcctca ttttcagtca
    24241 gactcgcaag gacatcccga acggaggggg caccggcagg aagttcgacg ggttcacaag
    24301 gcgttcccag atggacgcag cctgtgagag atactaaaag aacagcaggc aacagccaaa
    24361 gaagggcaga aggcttacag accgtgagaa acggccgaac gggacgaagc gcatatcggg
    24421 taaagcaggc gttatgcttg gtcatgttcg ggtgctttct cctgattcaa ttccagccgg
    24481 atactggaga tatagttggg agcaccctca agaataatga ttgtatggtt ctcccaggga
    24541 atgacctgcc cccgctcagg tatacatccg gcaagacggg ttacccagcc gcccactgtt
    24601 tctaccccgt tttctatcag cggaatatgc agcgcttcgc aaaacgcctc caatggcatc
    24661 tgtgcgtcaa caaaataggc gtcgggtccg atttttttga gttgtgtcgg cgccaggtcg
    24721 tattcgtcat ggatttcgcc gaatatctgt tcaagtatat cttccagcgt tatcagtcca
    24781 tcagtacccc cgtgctcatc ggttacaatg gcaacagatt gacgggcatc ccgcatcact
    24841 ttcagcacgt cgtccaattt cattgtgtcc ggcacatgga tgataggttt aataaaacgg
    24901 gcgatgtcct gacgatcggt tcttgtgtct ttaatcaggt caaaggcatt gacaacgccg
    24961 acaatctggt caacagtatc cgcatacacg ggcaggcgtg ttctgccgct ttcgatgaaa
    25021 aaagcagtta attcatgacg tgtagcagac tcgggcaagg caagcatttg catgcgcggc
    25081 accatgactt ccttggcact ctggttctgc agggcgatga ctgaatggat catctcatgc
    25141 tcttcggggt caagcgcacc ctgatcatga ctttcatcta ccagaatgcg cacgtcttct
    25201 ctggatcccg ccagcggctt catgtgctca ccttcccggc ccagcatttt gagcaccaat
    25261 ttagagagtt ggacgatggg ccaggtgatc ggcgccagca gacgatcaaa aaaagtgaca
    25321 agcggaaaaa gaggcagtac catctgcata ggaaagtgcc gggcgatact tttcgggaca
    25381 atttctccga caaaaagata aaccggggta gcgatcaatg ttgtccatcc ggcaccgact
    25441 gcccggctga gagaaatagt gccgataacg aggacaagat tggtgcctac cagcatcagt
    25501 gtgatcagtc gtcccggatc tttgtaatga gccagcagcc gtaccgctgt tttgttattc
    25561 tccttttcag ccaggtgtcg gattctgatc agattcgcag aatagaaaca ggtctcgtag
    25621 gcagcgaaaa aaaaggatcc aagtacacac attaggaaaa taaaaatatc catagggccc
    25681 gactattctt ccggcgcagt gcagggttca tgcatcaccc gtacaagcgt gatgcgcttc
    25741 ttatcgattt cttcaacacg gaagcataat ccctcatagg cgatatgatc tccgacagca
    25801 agaattgttt cagattgaga ttgaaggaac ccgccaaggg tggtgtgctc ttcatttttc
    25861 aatgtaagcc ccatcatttc agcaaagtcg gacagaggga aattgccggc gatcagcttt
    25921 gaattatccg gaccgtcaac gcacaaagga ggctccacgt cgtcctcttc gccaatatcg
    25981 cccaccactt ctctcagtgc atcctgcagc gttaccaggc cttccgtacc gccgtactca
    26041 tccacaacga tggcaatatg aatatgaagc ctctgagcca tggatacaaa atctgccacg
    26101 gtcatggttt ccgggacaaa gtgtgcccgg cgcatatatt gggtgaccgg gctgtcctgc
    26161 tctccgtctt ctgtgacagg gagcagatct ttggcgtaca aaatgccgca aatgcgatcg
    26221 agactgtctt tataaacggg catgcgtgaa tattcgtgtt cccgtacgat ggcaagcgcc
    26281 tcgcgtactg tggctgattc ccggatagcg acaatgtccg gccggggaga aagaatttcc
    26341 cgtagtgtca catcctgaaa ttcaagaatt ccctgaatca tctgccgctc atcttcttca
    26401 atagcaccgg cagcctctcc ttcagagaga aggattttaa attcctcatc cgtgagaaaa
    26461 acagcgggcg gcacctgact gaggcgggtt actttaaaaa gatatccgac caggctcatg
    26521 gagaagagcc gaaaaggcgt catgagcatt tccacgcaat aaatcggtac ggcggcggca
    26581 atggcaaagg ttcgtggatg agccactgcg agcattttgg gcagactttc acagaagaaa
    26641 agcagaatgg cagtagttac agcgaccgtc aacaaataag cagaagtccc ggaaagaagg
    26701 aagctcttct gcagaacagc ggtcatcggg cctgcaaagg tgatacttag cagcacattc
    26761 acgatggtat ttcccatcag gatggtggta agcaggctgg caggcttgtc cagcaactga
    26821 gcggtcaggc cgtgaatcca gctgcctgtt tcccgaaggg cacgcagctg cactttgctt
    26881 agagacagaa aagaggcctc gcaggaggaa aagaagaaag agcagagtat caggaaaaaa
    26941 ctggcaaata ttaccgaagg agtaataatt tcatgccagg gatgagagaa gtgccctgtt
    27001 ccgccggtat ctgctgcacc attggctctg gtagccaaac tgcagaagag gagaagggaa
    27061 aagatcagaa caaaagcctg aaaggcactc cggcctgtga acccaagtct tgctattcgc
    27121 ggagggtccc cgctggtatc gtcatccaag gaaatactgt gctcctgttc ctgcatattg
    27181 ttctgttaat ccagagaata cctattatat catgaatgcc aaacagtatc gaaaggcagt
    27241 tcaagataca gcgcctgctt ttgctgcatc agtgtacgtt tttccggtgt atcatgatcg
    27301 tatccgagca ggtgcagcat gccatggcat accagaagca gcagttcctg tctcattgct
    27361 tcccggacgt ccttgcaccg ggttcgcagc acatccatgg aaacaacgat atcgccgagc
    27421 acccggacac tatccgacgg gatttgcgca tccatgggaa aagcaagcac atctgtcgtt
    27481 gaagcgacat ttctgtactg tttgttcaga gaagtcataa atgcatcatc gcagaaaagc
    27541 acgctgactt ccagaggatc tttaatgcct tcacctgtca caatacattc cagaaggcgc
    27601 agcaatgtct ttttttgaat aatacggtgt acagtcgatt cgtttttgat atcaagtgtg
    27661 atcatgtggg tacggacgcc tcctgactgt ttttctgaga ggtattgtcc tgcaagggct
    27721 gcggttcttc ttttttgtga gcgttttgtc ccggataggc gatgcgtgta tgcattgcgt
    27781 tgaaaatgga gcgggcgacc acgtccctga tgatgcgcag ctgcttgagg gtaaggtcgc
    27841 agttgtcaaa ctgattgtct tcagcccgtg ccttaattat tgtgtcaaca aactggcgca
    27901 ctttttcctg tgtcggattg ggaatagagc gggctcctga ttctgatgca tcgcaaatca
    27961 tgaggatagc cgtttccgga cattgcggtt ttgggcccgg gtaacggaat tcatcgtccg
    28021 cgataatttc tccggggttt gttttttgcg ccaaatcata aaaaaaactg attctgcagg
    28081 tgccgtgatg ctcgagaatg ccgtcgatca cgggcttcgg aagattgtgc tcccgggctt
    28141 cccgtgcacc ttctataaca tgctgccgaa tcatacgcgc actgatttcc ggcggcatat
    28201 tgtcgtggat gtttttccgg ccgccctggt tttctgtaaa gtgctcgggt ttatgccgct
    28261 tgcctatgtc gtgatagtag gcgcataccc gggcctgaag cccgttggcg cctatggcgt
    28321 cggcagcggc ttcggcgatc tgtcccagca ggagactgtg tgcataggag gcgggtgccg
    28381 cgatagcgag tttacgcagc agctcattgt tgagatcgct gtactcaaga agctgcatat
    28441 cggtggtaat atcaaacaga cgttccagcc agggcagcag tgcaggcacc accagaacgc
    28501 ataagccgcc attcagcagt atcagaaaac tgcggcgcat gaagatctct ccaaagagaa
    28561 gatctgaagc aagcagacca gcgcctgcac ataacagccc cacaaaagtg gcgatcagag
    28621 cggcagcggt catatcattt cttttgcgaa ccttgtaaat gctgaaagcc ccggcaataa
    28681 tcatggcacc ctgcaccagc agcagccgcc aattatactg gtactgggcg gaaatcattg
    28741 cggcaagaag tacgccgaaa aaggccgccc gctgcgggcc tgccagaatg gcaaaaagaa
    28801 tgccgcctgc cgccaccggc aatacatagc ccgacggttc aaagtaagag ctgattcgcc
    28861 cgatgattaa agtgccacat aagatcagca gcgaaagatt gaaaacggtt ctcgatgaca
    28921 gaccgcgctg gtccacttga aaatgaaggc gtttgtataa tgcccaaaag acaagaagca
    28981 ccagaataaa atgcgctgta agggtattca ggattttttt gtagggatgc tgttcatcgg
    29041 acagaacatg catgtaggat tgcgcatcga tacgggactg ctccgtccaa cgttttcctg
    29101 catcctgaat aatttcgcct gcctcgatct cttttagaac gggtgctacc gattccatgg
    29161 caacagcacg tgcgttttca gtggattgcc tgtcttcctg cagcgtagca ataaggagag
    29221 gctcaaccag ggcttgtgag gcatcgtgga tacgggccca cgcttccggc atccccattt
    29281 cgctggcaag cgttttggca gattcagtaa gccgctgatt cagcagcgtt gctgctttag
    29341 caaggtcagg tacttcgcca aaactggtgt cggttgtaat gggaagttcg ggcaggttcc
    29401 tgtcacgaag aatgacgata tgccgtgcca gatctatgtt tgaagacacg ctgtgccgga
    29461 tgcctgtaaa aaggacaaat cgcaaagaag agagagccat ggcgcccatt ttattgctct
    29521 gttcaaaagt aaattccggc tgcagcgctg tcgggtcacg cggtgcggaa agatcagcgc
    29581 ccggagcact tgttaaattt accttgtccg gggtcagcca aagggcgagc aggtccggct
    29641 ccggaaaact ttcccacgca gtttcttctt tgagacgggt cgcataagca gtgacctcac
    29701 gctggaggac tgattcagga gtttgcgcag gctccgagcg ttgcagggca gcatgcagtt
    29761 tctcgggcac caggctgcat tgttcacgaa tcatttccag tctgtattga aggagccgga
    29821 tttgttcttc tgctgcttca ttgtggatgc gatagtgcac aggcactttc atggcttcca
    29881 gatcccgggc ttcctgtgtt tttttgatgt cggtggactc aaaataaaac atagcgcgaa
    29941 tttcacgctg cgccactgtg gatttgaggt cttcagggga gatacggtcg cggggcgatt
    30001 cccgggtcag cgccgcaatc gaaagcacga atacagccag cacaaaggcg atctgacgga
    30061 gcggcacctg aaagattcgt ttttcagtgt gatcctgccc gttgtgaacg ttaaaaatga
    30121 ccgaatccgc ccctgtgctg aggccggttg gggtgttgat gttgctggag aaagccataa
    30181 tgttctacag tgctgtttgg tgatagttga gggaaaggcc caatgggaaa gagggcatca
    30241 atgatgaatc tgaagcctca ttcggttttt tcttcgaagc gcactttatt ctgcaattgc
    30301 cgtgcctcat aagctgagac aactttttgc accagcggat tacgaattac gtcactgcgg
    30361 gttaaggcaa ccatgccaat accgtctatg ccctgcagcg tctcctgtgc atcaatgagc
    30421 ccggatttaa agccgcgggg caggtcaatc tgcgtcacgt cgcccgttac caccattttg
    30481 gacgactgac ccaacctggt taaaaacatc atcatttgct caggcgttgt gttttgagct
    30541 tcatcaagaa taacaaatgc attatccaaa gtacgtccgc gcataaaagc cagtggtgcc
    30601 acttcaatgg cctcctggtc aatcaggcgg ccgatccgat ccatatcaac catgctgtaa
    30661 agagcatcat ataaaggtct gagatacgga ttgactttct gagtgagatc gccgggaaga
    30721 aagcccaatc gttctccggc ctctacggct ggacgtgtaa ggacaaggcg ccttacctgc
    30781 cggtttaaca gtgcggacac agcaacagcc atggcaagaa aggtctttcc agtacctgcc
    30841 gggccggtta ctaatgtgat gtcctgagcg taaatcgtat ccagataaag tcgctgccca
    30901 tctgttcgcg gtcgtacttt cacatccggg cgaatgcctg ccggcttcgc agagagaagt
    30961 tctcctgttg tggtccctgc cgtgtcggga gatgcggtca acgcatattc gaaatctttc
    31021 aacgtaagag aatagccttt tctcacggca tccagcatgc cggacagcag ctcatgtgcg
    31081 gatcgcgcgg cattttgttc acctaaaatc gtgactttcg ccccccgatc cacaatacgc
    31141 acattgaaat gtgtctgaac taattttctg atggcaccat tatgtcccag cagtgccaat
    31201 gattcggatt ggctgttcag ttctatttcg caaatagggt tcgtagaata tcctttcgac
    31261 gcagtaagaa gtcggattta actctattat aacgtgaaca gcccgcagga gccaatctgt
    31321 actccatgcc tgagaaggac cgccgttgaa aacaagttga tttgcagttg caaacaacgt
    31381 atcctagatt ttaattcagt catgccattt gagctgaagc aataaaagga gtcttctcat
    31441 gacgatagaa cttccgcgct taaagtccta ctcccgcatg aaagaagtga tgaccatgta
    31501 ttacatgggc gcaaaaatgg cggaggggtc aggacagaaa atggcttgga taacaagcgg
    31561 tgctcccgta gagttcctgt atgccgctga cataacaccg ctctatcccg aaaaccatgc
    31621 tgccatgtgc ggtgcgtcac gtcttgccaa taccctttgt tccgctgcgg aggaggctgg
    31681 tttttcaaga gacctgtgtt cctatgcccg gacagatata ggcgccatcc tgaccggtac
    31741 cagtccgatt ggcggcctgc ccaaaccgga tcttctggtt tgctgcaaca atatctgcgg
    31801 aaccgtcacc aaatggtatc aggaactgca gcgccatttt gatgtgcctc tggtgcttct
    31861 ggatgtcccg tatcagtatg aaaaagaaga tgcggcgcat gtagtatctt atctgaaaga
    31921 ccagttgaag ggttatatcg gtaccattga agaggtgacg ggcaagtcct ttgattacga
    31981 ccgttttgat gaagtggtag ctttgtcagg tgaggccgcc gatttgtggc gtagcataca
    32041 gggcatgctg gcacacaagc ccgcgccaat gaattctttt gataccttta tacacatggc
    32101 accgatcgta acgctccgcg gcacgcaatt atgcctggat tattaccgtg agcttgaggc
    32161 ggaacttcgg gaacggattg aacagggaat aggggctgtt ccggtggaac gcttccgcct
    32221 gggatgggac aatctggcga tctggcatca gatgaaccag ctgtccagaa aatttgcaga
    32281 atttgacgca gcgctggttg tgtccactta tacagaaaat ttttcttacc gggggcctat
    32341 tcaggatccg aatgatcacc tgcttaccta tgcctccact tatattggag gatacataaa
    32401 ccacgggctg gacttccgtg aaaaagagtt gacgcgtatg gtaaaggatt tcagcctgga
    32461 tggttttgtg atgcattcca accgcagctg tcgcgcttac tcttttgggc agtatgaact
    32521 tgcccggcgt atggaggaaa agcatggagt accgactctt atgcttgaag cggacatgaa
    32581 cgattcgcgt acctggtcgg atgagcagat gaatatgcgg attgctgctt tcatggagac
    32641 tctcacaggg cggtgtgctg tcatgtaaag ggcccgggca gatcaggcag ggtactcaaa
    32701 caccatcgcg gagacgtgcc tctgccaccc cttacttctt taaatatttc tccagaaaac
    32761 gatacatatg ccagcgcaca cactgattga cttcctgaat gatatccaat gtgtggggat
    32821 aaccctccaa acgaagatac tcgtgaggga tttcaagtgc ttccagtcga gcgaccagca
    32881 gatcggcctg agctactggc acaattgagt catcagtgcc gtgaatcacc agcgttggtg
    32941 gatcgctttt gtccagatgg cgaattggcg aggcgagggc gtaactttca ggcgcctcgt
    33001 tgaaagtttt ccctccgaaa aaagctttca acgtaggatg atcatgaaat tcaaccggtg
    33061 tcagatcagt gggaccgtaa aggttgatga cagcctgtac agcactgctg aattccggat
    33121 agccgcctgt cccttccaaa gcctcaatat ctgaagaata ccctgtcatc atggcaagat
    33181 gtcctccggc agaaccgccg gcaacagcaa tggtatccgg gcagatccga tattctttcg
    33241 catgggcgcg catccagcgg actgcacatt tggcgtcttc cacgcatgca ggaaaagatg
    33301 cctctgaaac aaagcggtaa gaaatggttg caaccgcata ccccatttgt gccagacgga
    33361 ctgtgtagta tttgtaatca ctccgattgc cgcttgccca gcctccgcca tgaataaaaa
    33421 ggatgccggg aatatcggcg tcctgatcac ggggaaggta aagatccaga agcagtgcac
    33481 gcccgtttac attcccgtat tcaatatctt tcttttccag tacgtttccc gggacaggaa
    33541 caggaccctg agggttcaga agtgtaattt catcttttgc cgctgcaata tacaaatcac
    33601 tttctgaatg gatgtccggc ggtagagcag gagcttcggg aagcgcatgc agctccggtt
    33661 ctccttcaat cagctcggca taaacggaag cggtaacatc cggaaggcag gggagaagga
    33721 gtgaaagaaa aaggcatcca gcaataactg tccgcgaagg aagaaaaaat gaattctttc
    33781 gcaagattcg ttcctttttt aaagtggttc acgcagtaaa gagaaaaacg ttcctcgctg
    33841 gttaccccga agcgattatt cttgtactga aagatcggcg ggagcgggag agtcgagctg
    33901 aaccagcatg acttcaagcg gttccatctc gcggggaaat tccacatcgc gggcaagaat
    33961 taaatcgcga ccgccttccg atgcgcctgt gatgtggacg cctaccggag cacgacgcaa
    34021 atttatgatg taaagatact cctggttttg aatacggaca tagcgggaac gcaccccttc
    34081 aagcggataa ccgtgggcat tgatcgggcg gggaatcagc gggagctttc cccgatccag
    34141 caccgcatcc atggcatgca ggtactcagt cggaagattc attccccgca ccagtacggt
    34201 attggggctt gtgcggatta catcatttcg ggatatgccg ctttcgttat aaggaatagg
    34261 cgtgcccacc cgggcgacca ttccgcctgc ttccacatag gcatcaatct tcagaaaggc
    34321 ttcatctgat attgccattg tttccggcat gatcaaaact ttaagtttat ccagaccgcc
    34381 ttttaagatt tgaccctctg tcaggtaatg aatgggatat cccgaaaagg aagagccttc
    34441 aaaggcaaaa cgggcagact ccagatgagg aacaccgtcg tccatgattt tggcagcttc
    34501 actgaaaaga atgccgattt caggcgtgtc ctgttgaaat gcgactacaa tatttcccag
    34561 cctgttaata tccattgtgc ttctgacaaa ggcttcgaaa gcatcggggt aatcaaaaac
    34621 ggatgagtca tgagcgagga caaagcccga ggcccccgat atgacggact cccagagggc
    34681 agcagtgaca atacgcaccc gcatttccga ctcttttatt gtgctgacat cgatatcgcc
    34741 ttttaaagga agcagcgctt tgtccggctg gtagaagcgc atcaaagtca gataggcgtt
    34801 gggaattgga tagttcagtg catagacccc gtcaccgggc tcaaagcgaa ctgtgcatgc
    34861 ctgaagatcc ataatctccg acatatcgga tcttcctgct gtgcttcggg tttctcccgg
    34921 caaaaatgca gtgtcgggtt gggtcagcgt cagggggatt ttcggcgcca gagaggacag
    34981 tgactgcttg aactgcgaga aaaaatcagc aatcagggaa cggtgaaagg tctgccattc
    35041 atattggtag gcgcgctggt tatggtagct gtgctctttg ggattctccc agatactgat
    35101 ttcatcataa gaagcaagtt tggcatgcca cagccggttc agcgtcatcc gatccggatg
    35161 cttttcttta atggtgttaa tgaatgcctg ccgcacttct tcgccatcgt aatggaagcg
    35221 cggattgtcg gcaagactga cagcggctat gcggccggat cgattctgga gatgaggcat
    35281 ggctgcaagc aattgatctc tgtatgcctt gttaaagtcg acatgagcaa ggtttacaaa
    35341 acccgggctt atgattgccg gttgtacatc cattagcttt tcaacatctt catcctgctg
    35401 aaactgcagt gcccagaaca gttgttcgtg ctcagcagca gaggcagcgc gatccagtaa
    35461 agaagcgatt tgtgatttgt ccagcgtaat ggggcaattc agcactaaaa aattaaggtt
    35521 gtaccgcgag agcagtgccg cctgattttc taaaaatgcc tgttcttccg tggtggtttc
    35581 aggattggga agccccaggc cgaagagaaa aagtgcctta tcagagcaat agaacccgcc
    35641 ttgccgcaat tcgcacgtca gatgatccgt ggtttctgtc atgcacagct cgggtcgctg
    35701 ggtgctgaaa acgagggctg cctgtaagga tggaagtgct gtttccaaat actccatatt
    35761 tcgggaaacc actgaccagt tgccttgctg caaggcatcc ttcgctgctt ctatggcacg
    35821 gtgtgctgtt tggacactca ggctgagata cggtgttgat accagtgact gaccatgctt
    35881 tttggcaata ccctcagaca gtgccgtgct ttgctgctcc agtttgctta gcgtttcaat
    35941 ggactgtgcc agtttctgct gccaggcttc actgctgata cgacgcatgc aggtgtgagc
    36001 ggataaagga ggaaatgcat ctgaatagtc gacttgaatt gacaacgtat agcgcccggg
    36061 tgttaaagac aagccgctcc attcgaaacg aaaatcattc agaccctggc gaagatcagc
    36121 aatggactcg aggcgttgcg caagattatt ttgcacatcg cgcacttgga gaatcacggc
    36181 ggttttctga tacggcacag tcgcagcaat gctgataccc aaatctatat tgtctgctgc
    36241 cggaactgac ggcaaaaccg gcagatcaat ggtgactgta ttggattttt ccactgtcca
    36301 actgtcaaac cagatttctt ccagagaagc tgcggtgtct gcggtggcgg gcagcgctga
    36361 tgcacagagc actccggaga gaaacagcag cggcagaatg gcaaaacgga gggatctggc
    36421 ggttggcata gaatgtatcc ttgtgagaca gtgcaggtac agcacagccg ggattctgtg
    36481 taagaacgta tcgactccgg cttatgttaa cgcagtcgta tctttgtatc agtgaaaaca
    36541 ggcggttatt tatcggggca gtgcggagga aatatcatca agttttgcac tcagatattt
    36601 gacagcgaga ggctcgagcg cctctttcgg tccgatcagg tgctgtgtgc gatgtatatg
    36661 agtcagggac tcgcccggct gcagggatgc ggccggagaa gaactttcca attcataaaa
    36721 gggtcccatc ggctttttcc ccggcgcagg cggaccgtca ttgtaagcat tgatggcatc
    36781 acccgaaaag ggctcttcct gaagttccca catggagttt acgtatttat ctgtgtcccc
    36841 cggcagtgtg tactgaacca atgtcagcgc ctgcaagccg ctgtcataac tgccgagcca
    36901 ctccgttgca cgcttggggc tgattccgat tttactccgc atggtgccat ctgcgctgaa
    36961 aaacagaacg ttgtctctct ggacgagtcg gtcttcaggc actttgccaa aatacttgtc
    37021 attgaccttg gcgccaagag cttcgtcact tccttcccgg aaaggaatga caatcgtggc
    37081 gtccggagag ggactgaaca tgccaagaat ccagatggac agcatccctg tctcttcgtt
    37141 ccacgctgaa tctcccagat tggtgatggt gttttccgat tcaaaagcaa cactttgcgc
    37201 ctcgtccgtc aggctttccg ccagacggat gacacgccgt atctttacat caaaggagct
    37261 gcccgaacgg tttgtgatgg ttgcgcggtg ctcaaaagcc acttctgaat cgctttgctc
    37321 gacaaccgca taagcaaccg tatcaatgac aggtggcgtt tgccagtgct caagatcgaa
    37381 aggggcaccg gcttcaaaga agatggagaa ctgcccgcct tcaggaccga gccagaaacg
    37441 atcttctccg ccgaaggcat tgatatgggg ctcaaatttt tttgatgcaa tcagttctct
    37501 gttaatccag ccgaaacttg ggccgttggc accggatgcc gtactggtca tgacccggcc
    37561 ctgataagcg ggcaccacag caacctgtgc ctgcccgtcc ggacttttga gtacaattac
    37621 atccaggtat tgcttcagaa aatccacatc ttcctgaaaa gtggctgcat gaccatataa
    37681 ggcgacagca cagacgaaca caagactcaa aaacatttgg cgtttcataa gatctccttt
    37741 atggggaatc aacagtccct tttccttaaa aaaatcttta gagcgggttt atttctacaa
    37801 gttttaactt gtccactttg ccattgggga gcagtggcaa ggttgcgtaa aaaaagacac
    37861 gctgcggaat tttgaagtgg gcgagatgat cccggcacca gctgagcagc tgcatctccg
    37921 ttaactttga tcctggaaca gtcatcacat gggcatggca cacttcggtg aatacatcat
    37981 ccggcacggc ggtaatggcg gcgaaaagta tttgcggatg cgattccaga gtcaactcaa
    38041 tttcaaatgg aaaaatattt tctccgcctg ttttaatcat gtcggtgcgc cgcccatgca
    38101 gcgtcagatt gccttcctta tcaagactgc ctaaatcatg ggtatagtac cagcccgcag
    38161 catcgagaac tgccgctgtc tgaatcggct cgttcagata ccctttcata acaacagggc
    38221 cgcgaacagc gatttcgcca atctgacctg gcgggagtgg gttgcgctgc tcgtcaacta
    38281 tccgtaattc acaattagga tagacaggcc cggtagactg gtctgaaacg gggaaaggcg
    38341 cttttttttg cgtcgccgtg acaaaaccgc acaattctgt ggaaccgtat ccggtcacca
    38401 gaatggcacc tgtccggctg cagatgcggt gcaacgcctc cagcatctct ttggaagcag
    38461 gggagccgcc ccagacgaag gtttccacag aatcccatct ggttaaatga aaggacggtt
    38521 cccgtaactg caggatatac atggccggaa cctgtcccag taccgtgata ttttcttttt
    38581 cgatgatctc caggcttgct tttgcgttaa agtccggctg catgactatt gtggcgcctg
    38641 caaaaagagc acagtagctg atttcaacat cagctgccac atggttgatt ggaaaatgaa
    38701 gcaggatcct gctgttttta tgaatgttgt acagtgtgtg ctgctgtgaa acattgctga
    38761 tgacacttga atgggtgtgg agtacgcctt taggattacc ggaagaaccg gaagtgtaca
    38821 taagcagcgc attgtcacca ggagaggccg cagccagacg ttcttcataa agggatgcag
    38881 tatctgaata gtcttttttc aggattgtat gaaaagacgg cgcatcgcca tgctctccat
    38941 caatcaggat aatgtgctca atgcttgcca gttcgaaatt gaaggtaaaa gcccgctcaa
    39001 gcaggtttgt gttgtgatag cgttcctgcg tgatcagtac agtcgggcgg catcctctga
    39061 ggatacggcc catttctcct acggtatatc ggggcgatat gcctgtccag agggcggaga
    39121 tttttgatgc ggctaaaaaa gatatcacaa attcagggcg ggcagaagaa atacacgcca
    39181 cacagtcgcc gcgacctact cctaaagcga gaaatgcccg ggcagcctga tccacttgac
    39241 gggccagctc tgcccagctg aggcgcgtgt cttcaaacac cagggcttct gcatctccct
    39301 tgacagacgc ccagtgctcg acgcactgcc atagaaatct tttagtttca gccatacagt
    39361 taccttaatt ttggcgtgga atgagactat cacgcctccc gcttgcaagg caaattaacg
    39421 gggattgcct caacgaaaga aagggtgccg tgtgtccggc tgtgtccttt ctttatttgt
    39481 gtatcagata ccacttatac gcccaccgaa aaatgctgtg cttgcaggta cgcagaaaat
    39541 accgaccttc gcccggcaca acgggatttc tgcggaacag ccgtataatt atacggattt
    39601 tctgcctatg ctgaaatatt taaatccatg ttcttgcata acgaccggcg tgtacagatt
    39661 tctcccgtca aagataacag gctcccgcag aagcgaagcc atgcgggcat aatcggggcg
    39721 cctgaactcg ttccattccg tcagaataac caggccgtcc gcattttcaa gggcgctgta
    39781 atacttgggc gccatttgaa tgcggtttcc gtagcgcgcc gatacttttt gtcccgccac
    39841 cgggtcatag acgacgactt ccgcaccggc ggcacacaat ccttcaataa tatccagaga
    39901 aggtgcgtcc cggatatcat cagttcttgc cttgaaactg acaccccaaa gagcaaggcg
    39961 tttgctgctt atttttgagc cgtaatagtc cagtatcttt gcaagaaatt gttgtcgccg
    40021 ccggttgttt actgcgctga tggcggtcag taagtcgtgc tccagccccg cttcccgggc
    40081 gagtcgttcg cataccgcca catcgcgggg aagccctgaa ccgccaaagc caataccggg
    40141 aaaaagaaaa gtaggcccga tgcggctgtc cataccgacc gcctcccgta tgtccgcaat
    40201 gtcacagtcg tatgcttcgc ataaatcagc cagctggttc atgagcgaga tgcgggatgc
    40261 cagcatggcg ttggttgcgt attttgccat ctctgcgctt tccggactca ttgccagaaa
    40321 cggtttgcct gtcctcaaaa aagggctgta cagttcgcgc atcatttcct gaacacggac
    40381 atcccggcat cctacaataa tgcggtcagg gcgcaggaag tcatcgacag cggagccttc
    40441 tttcataaaa tcaggattga ccaccacgtc cccggcctgt ctgattttcc agatctcttc
    40501 gatctttttg gctgtgcccg gaggacaggt gcttttgttg acaataattt gataaccagt
    40561 gcgttttttc gcaatctctt cggtaacgct caggacattg cttaagtcca cctgccccaa
    40621 ccggtcggca gggctgccca cacacagaaa caccatgagg ctgccgtcta gcgctgccga
    40681 cagatctgtt gtaaagctca aacgctcctc ttcaagattt cggatgatca gctcttccag
    40741 acccggttca tggatcggga gttttcccgc ctgcaactga ttgattacag ccggattctt
    40801 gtcaaggcaa acgaccgaat gtccattttc agcaaagcag gttcctgtaa ccagtccctg
    40861 atatccgcag cctagaatag ttattttcat ataaaactcc gatattgaaa aaatgcaccc
    40921 ggacggcgct gcgcacttct gcctgatact tccggatatg gtaacagtat aaaggaagga
    40981 ttcgcaacgg agtgagaagg acggcgaagc atcgcctttt tccgttgtag ggaatttcag
    41041 cctattttta agccgttctc agttgaggtg tcaacagtaa atatgctaca ttaaagacag
    41101 cagcggtaag aagtttccct gacttctctt tgtcattccg ctgtattttc ttttttttcc
    41161 cgttttgtgc acactacagg atcggagaat ccggcatgca agattggatg cgtaaacatc
    41221 ggcggttaat tttctttttt atttttattt ttatcggcat accctttgtt tttatgtggg
    41281 gtaaaccgac atcgccccac ggcaatcagg ctgccgacag tgcggtgctg gcgcaggtcg
    41341 gcggcgttcc cattaaagaa tcggagttca ggcgcgggct ggatgctgcg gcgcaagcaa
    41401 gaagccgcca gggcggtgat cgtccttcct acaccgatct cgataaagac ggcacggcgc
    41461 aggcagtact tgaacagctt gtggatgcct ccctgctcag aatccgggca gctcagcgca
    41521 atttttcagt tgacaaacgg gttctggaaa agcagatgca gcaatggggg atgtttcagg
    41581 atgaaaacgg tgttttcaac catgagatat ggaatgaatg gatagccagt gttgaacggt
    41641 ggaacgagat ttacgacgaa cttgaagaag tagtgggtcg ccagatgtat ctttccgctg
    41701 tgtctgcgcc tgcacaccgg ataatcgccg caaaagcaca aaatgagctg aaatccgata
    41761 acatcaaaat taaagttaag ttcgcaaaaa ttgagccgca agtggcggtg tccgacgatg
    41821 atgcgcgcaa gcactatgac gaaaatccgg aggcatacag actgccggaa cagtacaagg
    41881 ctgaatttat agcctattct ctgactccgg atgtgccgga gcaggcaaat gaacttgttg
    41941 cacgcattcg ggctggtgaa gattttgtcg ctgttgccaa agaatattcc gggcaggatg
    42001 aggaagacag cagcgatctg gggtggagtc ctgtaaatcc ggacgcagcg ccctatatga
    42061 aggctctgta cgccttggaa ccgggagcag tgagcgaccc tgtatgggga ccgctggggt
    42121 accatatttt caaaaacgaa gaaatacgaa tcaaccccga tacggaagaa aaagaagtgc
    42181 acggaaggca gattgtagtt cctgccatga tgactcctga agaagctgct attcgtgaaa
    42241 aggcacttgg cgaaattgct aaaaaaattc aggatggcga agatgctgcg tccgcagcag
    42301 cagcggccgg tttgtccctg cagcagactt ccttcttcgg gcgtaccagt acggaaatag
    42361 aacctctgtc catgaatgat ttgtttacat tccgctcgca agtgattgcc cagaaagata
    42421 ctccctggac agtcctcaag ggtcgggata acttgtttat tacgcacatc attgagacgg
    42481 cacccggtac aattcctgag tttgcggaag tcgaacagga tgccagagat aatttcattg
    42541 ctcaggaaaa acgctctgaa gcttatttgg cacggataga tgagtatgcc aaacagatta
    42601 aagaaagcaa attaaaaatt gacagcctgg caggcgcatt tcctgacata gttcaggaga
    42661 ccggagagac acctgaggcc tttacccgta aggatacgtt attccagcag aagatctatg
    42721 tgcaggccaa tgagattgtt gctgctttgc aggatgccct gcccggcgat atagccggtc
    42781 cgatcaaggg cttttttggc gatgcctggt tctttgagct cgtcgaaaaa acagagccaa
    42841 ctgaagagga attagccgct ctttcggatg aattgaaaaa cattgaagcg cggctcgccc
    42901 aaaatgccga gtatgaaatc atgtccgatt tcatgaaaga tctcagagaa agaatgcttg
    42961 ccagcgtggc atacaagcag aataacgatg tgttggatcg tgtgctgagc cggggaaaat
    43021 atgcgattga agagcgggag cctgagaaag agactgcacc tgtggatgcc gccgcttcag
    43081 cagcagactc gtcgcccggc gagagtgccg gagcggacgc tgcagcagca gccgaaactg
    43141 ccgatgcatc aggatcagaa gaagataagt aggctgtaaa gtctgcagaa tgccgagcat
    43201 acgccgacga gctgattttt tcccggactt gggtgttgcc aggtgcctgg aggtggcgtt
    43261 ctgtaacaag gagtaaggat aatggaaaaa gttgtgatta tcggaggcgg tcccgcagcc
    43321 tgtacagcag cgctgtacac tgcccgggct gatctgtcac cggtggtgct ggaaggtgaa
    43381 ttcagtcagg atattcttcc gggcggtcaa ttgatgacca caacggaagt ggaaaatttt
    43441 ccaggttacc cggaaggaat ttccggcgct gaactgatgg ctgacctgaa aaaacaggcg
    43501 gaacgttttg gtgcgcggtt tgaatataaa acagtaacac gtgtgaattt cgccgacagg
    43561 cctttgcgcc ttttctgcgg agagacagaa atcagcagcc atgctgtgat tgtggcgaca
    43621 ggtgccacag cgaaatatct cgggctggat gcagaaaaac gctttctgaa taacggtgtt
    43681 tctgcctgcg ctacctgtga cggggcactt ccccgattcc gcaacaatgt agtcgttgtg
    43741 gtcgggggtg gagatacagc agcggaagag gccttgtttc ttgccaattt cgccgggcgc
    43801 gtgcatgtta tccatcggag agactctttt cgtgccagta agatcatgag tgatcgtgtg
    43861 atcagccatc ccaaaatcac agttgaatgg aattctgttg taacagatat tcttggcgat
    43921 gatgaaaaag gggtgtccgc tgttgtgctg aaagacacgc aaagtggcga agaacgtaca
    43981 ctggcgtgtg tcggatattt tgcagctatc gggcataaac cgaatacaga cttgttcgcg
    44041 ggagtgctcg atatggacga cgcaggctat ctcgttaccc gaccagacag ctcttacacc
    44101 aattgcgaag gggtctttgc ctgtggtgat gtgcaggacc ggatctacag gcaggcaatc
    44161 acggcagcag gcagcggttg catggctgct ctggattgca cgcgatggct tgaagcacag
    44221 caaattgact gatttctttt ctcttttctt ctgacggctt gaaaaagtct cctcttatga
    44281 ccgtgctttt ttcttcaaaa ctgtccatcc gtgagatggc tgtagtgtgt cggcagttaa
    44341 gcaccgctta caacgccggg cttcctatta tttccgcatt gcaacttgtt gtagaccgcg
    44401 gtgcgtcaat acgtgcacgg cgtgcgttgc ttaccatggc ggagctgatt cgcaacggcg
    44461 ccacccttgc cgatgctgca cgcgcgcagg aagcaacctt tccggatctg tttattgtgg
    44521 tgctcgcagc aggcgaaacg ggaggaaaac tgggcgcgct gcttaatgaa ctcgcggatc
    44581 attatgaaga aatggcaaag atcgcccggg cggcaaaagt tgccatggtc tatcccggtt
    44641 tgcagctgct gagcgcctgg tatctgggca cttttgccct gaatatcatg aaaaccattc
    44701 tggatccgtc aagaggacgc atcgtatttg cggattacct gtcttcctat gcttcttttc
    44761 agataaaaag ccatctgatc gcactggcag tgattgcagt gattttcatc ctgaagcgtt
    44821 tcgggatgct cgattctgga atttctattg taaaaaatca tgtctggcca ataaaccaga
    44881 tttccagaaa attttccatg gcccgttttt ttcaatgttt tgctcttttg ctgggcgccg
    44941 gtatcgaaat aaaacaatgc ataagcagaa gtgccgcatt gacattgaat cgcattatgg
    45001 aacgcgatct gcttcaggcg attcctttta ttgttcaagg aaaaacactt gcggaagctt
    45061 ttgcacgctg taaaagtttt acccgtgttg accatgaaat ggtcggtgtg ggtgaattat
    45121 ccggtgagct cgaagcgacg tgcaaaaagc tgtctgaata ccatctggca gaagcgagca
    45181 gtgcgcttaa agtgggaatg cgggtcatgc aggttcttat aaccctggta gtcggagccg
    45241 tgatcggcta tattgtaatt tcgttttacg ggagattgta tggatccatg cttgaccttt
    45301 gacagatcac tgccgaggta ccgggactga atggagtgta tgatggatat acaagagaaa
    45361 ctcaaaaact ggaacgaaga aactgttgcg gaagacccca gagactatac gaaactcatc
    45421 tggcttgggg tggggattct gattgttgcc atgatgtttt ttttatactc cgggagaacc
    45481 accaagcctt tgacgaccag cgttcatgtg cgccatattc tggtttcgtt tgacccggcc
    45541 gatccggttg accgcgggaa ggcgcacgac cgtatccttg agctgaaaaa acggctcgag
    45601 gcaggagaaa gttttgataa gctggcgcga aagtattcgg atgacactgc gtctgcaaaa
    45661 aggggcggtg atttgggatg ggcacctaaa gggatttacg cagcgccttt tgatgaatat
    45721 tgctggacag gcaatatcgg ggctatcagt gatgtgatcc ttaccaattt tggttttcat
    45781 ctcgtgcaga tagaggaaag atacattgcc ggagctgacc tttacgaaaa agagttggag
    45841 aagcgtgccc ttgagaaaat gaaagaggaa gccgccaaag gtgttgcccc ttgaaggggt
    45901 atttctgcat gctgttcaac ttttaactgg agaagatgct ttgattttgc aagaaatgat
    45961 tgcccgacta attgtttccg gattcccgga tgttgaagat gcagacatac aatgtacagt
    46021 accaccgtct ccggaactgg gcgatgtctc tgtgcctatg tttcaacttg ctaaaaagct
    46081 tcgcaagcct cccgtcgttt tagccaaaga ggtggcagac agcgtggatt tttctgcaat
    46141 agcggaatct gtttcgctag caggaccgta tttgaatctt attcttaagc gtgattattt
    46201 tgcgaaacag cttttctccc gtgcatttgc aaaaaaggaa aaatggggaa gcaatgcgag
    46261 tggcgccggt aaaaaagttc ttatagagca tacgagtatt aatcccaatg ccagccctca
    46321 tgtgggcagg gggcgctgtg ccatgatagg ggacagcctg gttcgcctgc ttcgttttga
    46381 agattatgat gttgatgtcc attattatgt gaatgacatg ggcaggcaga tcgcactgct
    46441 tgtgctgatg atggaagaac tggacaaccc tacttttgat gaaattcttg agatctatgt
    46501 gaaagccaat gtccgtgccg aggcagatcc ggaatttgca gctcggggct acgaactgct
    46561 tgcccgtatt gagtccggcg acgaagagat acggaaacgt tttttcgata taacggagat
    46621 gtgtttaaag gggcaacttg ctgtactgca gcggataggt gctcaatacg atttctttga
    46681 tcatgagtcg aactatgtga acgatccgcg gctttcggtg atcgaaaaag cactgcaaag
    46741 caaaggggcg ctttttaccg atgaagaggc gcgtctggtc gtcgatctgt ccaaacttgg
    46801 gtatcctctg gaggaagggc gtttttttgt gctgcgccgt gccaatggct cgtccatgta
    46861 tgggtgtcgg gatttaattt attccattga aaaacaggag aagggcgctg attataattt
    46921 aatggttctg ggagaggatc ataaactcta tgcacagcag cttgccttga ttcttgaagc
    46981 tgccggatat ccggctccag agaccatcta ttactcttac atccttctta aagaagggaa
    47041 gatgagtacg agaagcggca aggtggttct gctcacctct gttctggatc aggctgaagc
    47101 gatcgctttg gaaaaagtca ccgaacagtt tcccgatttg gcgggagcgg aaagagagga
    47161 tattgccagg caggttgctg ttgctgccat ccgttttgcc gtgctccggg tgaagccgag
    47221 taaaaatgtt gtattcgata tggagacggc gttgtctttt cagggggata caggccccta
    47281 catccagtat tgttgtgccc gcattcgttc cattctgaga aaatgggatc aggcgcttcc
    47341 tgatctgagc tcctgtgcct ttgcctttga tgaagaaggt gagtggagac tagtgctgaa
    47401 agtcgccgat tttcctgata ttgtaaaaga ggcattgctg ctgcgaagct gcgccggaat
    47461 tggaaattat ctgattgatc tggcccatgt attcaccacc ttttatcatc agtgtcctgt
    47521 cctgaccgca aaaacagagg tgcagcagga atccagaatc cttttatgtg ctcttgtact
    47581 gcagaccttg tctaacggcc ttgctctgct gggcattgaa acccccgaac gcatgtaggc
    47641 ctttatggtg cacgctggcg gcacagctga actgtatatt gcttaaaata cggcaatttg
    47701 ctaaactttc atgcattgtc tttattccag aaacaccggg ctgaaacaga atacgatgaa
    47761 ccaatgtcag aactgcaaag cagccaggtg ttctgtcatc ggcctttatg gagtctgtca
    47821 tgattgagaa acgatatatc gaccttattg catcagacga gaacgttacc ccggaacagg
    47881 tggagtctgc aatatcactt ctgaatgcag gcgccacggt gtcctttatt atgcggtacc
    47941 ggaaggatgt cacgggcggt ttaagcgaaa gtaaactgga gcgtatttca gatcgcaata
    48001 agtactatac ccagctgtac tccaaaaaga aatctctgct gcacagtctg gaaaaaccgg
    48061 atccggggca ggaagaaaaa caagtcgtct ctgaagaaat tcaggctgcc attgatgcgt
    48121 gcgatgacat gatcgtactc gaagatctga gtcttccata taaaaagcag cggaacaatc
    48181 atgcagccac agctgtggcg aaaggactgc tgccacttgc taattacgtc tgggcacagg
    48241 cgccctcatt gccgcctccc gaaatgtatg cgcagtcttt tgtttctgcc gaccagaaga
    48301 tctattccgt cgaagaagca ctggaagggg caaagcatat tctcgcagaa tgcatagcca
    48361 tgaatgtgga tgtgcgcaaa gagcttcgta aagcactggc ggaagaggga cgcctgcttg
    48421 tgcatcccac ccgtgccgag gcagaaaaga aatcaaaata tgacgcctac aaagatctga
    48481 atgttctttt ggcggaactc gatgctgaga aactcctgac catcttaaga ggtgagtggg
    48541 aagctgcttt gcgggttgaa ctcgtgatcg atgatgaaaa gatgcttgcc ggtattatca
    48601 accgctttgt caatgacaaa gaaagcgcat atgccgttga gttgacaggt gctgtgaacg
    48661 atgcttaccg aagattgctc cgcccgtctc tggaagtcga ggtcattgca caggcgcgcc
    48721 ggaatgcgga ggatgccgtt attgaggcat gccgcgagca ggtgcgtaat acacttcttg
    48781 ccgcaccggc aggggaagtg cctgttattg ccgtgtgctc tttatctccg ggcgagtatt
    48841 ctttcgtgct cgtcgatgaa agcggtgccc ttctcgaaga tcgtcaggtt gagagtccca
    48901 atgaagatgt cttgcatgca gatgctgaag cagcgctgga agcacttctg cacaaagcgc
    48961 ccgctgccgg gattgccgta agcagcgggc cgggaggcag aagcctgctg cggcttatac
    49021 aggcctatct caagaaaaaa gggcaccggc aaagttatgc cacactggtg tctgatgcag
    49081 gattggcggc ttatgcatcg tcacctctgg ctgccgaaga actgcccggg ttgaaagagt
    49141 ctgtccgtgc tgcggtttcc attgcccgga gacatcagga tcctttaaaa gagttgatca
    49201 aaatggatcc gattctgctc gttccaggca aaattgcttt tggcgtaaac aggcggcggc
    49261 ttctggcgag cattaacaga accattgaat ccacggtgaa ccgcaacggt cttgacgtaa
    49321 ataaatccac tgtggaaatg cttcggtata tcagcgggct tcagttcgga ctggcacagg
    49381 caattgtaaa aacacgcgaa gaaagaggcg gcttcaccaa cagaacgcag ctgatggaag
    49441 tgccgggctt ggacaacaaa atcttccagc agtgcgccgg atttttgaga attatcggag
    49501 gcgaacaaat cctggacgcc tcatccattc atccggaggc gtatggtact gtcgaaaaac
    49561 tggctgcatc actttcactg tctgttgccg aactgaccca gaacactgaa cctttgaaca
    49621 gcgtcaggct ggaagacttt gttgacggta tcatcggtgt gatgactctg gaagatatct
    49681 gctacgatct gggacgtatc ggcagagatt caagaaagaa attcaaagcg cctgcaagct
    49741 ttgtgacgct ggattccata gaggatttgg agccgggcat ggtgctggaa ggactcatca
    49801 ccaatgtgac agatttcgga gtctttgttt ccctcgggct tcctgaggag gggctgctgc
    49861 atcgctctga aatgccgcgg ctggatatca aagcattgaa aacctcgatc ctcacaggcg
    49921 aagttattaa agtccaggtg aaagaagtgg aaccttctaa aggcaaaata ggacttacga
    49981 ttcgaaacgt tgcaaaactg ccgttaacac gtccaaccgg agcaggacga ttccccaaag
    50041 agccttggga tcagcgggag cgcggacgcg gtgaccggac aaaacgtaga gcacccaaag
    50101 acagccagag agccccgaag cagcagaagg aaggactaca cgaaggcagc ctgatcaata
    50161 cggcattggc agagcagctt gcggctctgc ggaacaaaat catttcaaag tagcggcaga
    50221 ttgtctgctt cgttggagat cttccggcgg aactcaggaa aagtcgctgt ctgtctcagc
    50281 ccttttgatt aattcgcggg ctgatagtgt tccatctttt ttcgcagatc gtcctgcgcc
    50341 ggatccagac gcagagcgcg acgccattgc tccagcgctg cctgtgtatt tccctgagcc
    50401 tcataaatat cacccagatg cctgcgaagg acagcgtcgt ctgtctccat gccgtaaatg
    50461 gctttcctga ccagttctgc cgcctgatca aagttgccca gtcggtagtg gatccatccc
    50521 agactatcca gataaaaagg atcgtcgggg gaaaggctca gtgccagcgt aagcagtttc
    50581 agcgccttgc ccagattgcg attttcttcg gcatataaat acccgagaaa attcagcatg
    50641 gtcgcatttt gcgaatccaa aaaaagatag gcaagcaggt gtttttctgt actttccata
    50701 tccttgaggg cttcactggc gattgcctga tggtacagaa caaatttctg cgcttcgccg
    50761 tccgcctgaa tctgattaag aaggtcaaga gcttcgcggg ggcgtttcaa ataaagaaga
    50821 aggcgcgcct ggagaaatgc agtgaatctg ccgatagaag gagattcctg aaagaaggcc
    50881 atctgtgaac aaagcgcatc tgcaagctga ggaggtgcct gcagggaaat aagagcgtcc
    50941 tcgatgcggc tgcattccag ttctatttct tccaaagaca aaaacacttt atcctgaccg
    51001 ctatattcgt tgggtgccgc atttgccaga cggccgtaac aactggcaag gaaaggggca
    51061 ttgccttcaa gagcagattg cgcagcgccg gaaaaattca tttccagccc ctggacaaga
    51121 gcccattgca gaacattttt cgcacccgct tcctgattct ccagcaggcg gtgcatgata
    51181 gaacgggctt cttccatccg gcgcaaatgg agcattgttc cgacgagata ctgcaaggcc
    51241 tgcatgtcgt tgggtcgctc cgccaaataa agataaaaag catctttgct ttcgttaaat
    51301 ctgtgaagat tggaaaggtg aatagcttgc attaggcgtg cctgggtcac gaggggctcc
    51361 aattcaagta cccgggtgaa ggcagcgcat gctcccgcat tatcattgat ctttgtcaga
    51421 ttcatcccca attgaaaata taaagcagca ctgtccggac tgttttgtat cagcttttca
    51481 taaagttcga tggcagctac aagatcattg gttttttcat acagatcagc cagggcgctg
    51541 tatccgagca tattgtcggg cttcagggct atggcttttt cgaagcagtc ccgtgcaccg
    51601 tcaaaatctc ctgctgcctg cttcatttct ccctgaataa cccaaaattc aacaacctcc
    51661 ggcgaataac gcagaacctc ttcacctgtt gcaatagcct ggtttaagtc accttgcgtg
    51721 agataaagcc ggatcattct gagccgggga gcatgggctt ccggggcaaa ggcaagaaca
    51781 cttttccagg actccagcgc ctcggcgaac ttgccctgcc gttcatacaa gactcccgct
    51841 atgtgatgag cgtaagcttt gccttgcggt ctgatcggca caactttccc ccagtatact
    51901 ctgttgctct gtcctgggaa atgagcacat ccggtactga agagaagcag caggaataaa
    51961 caggcgttat ataaatattt tctgggcgtc ataagggctg atggctctct tcagtgtccg
    52021 tttctatgcc tgaacaggcg aaaaagtttg tgttattcag aatcctgccg gccaggattc
    52081 gttgtgaaat ccaaaaaagt cttttccgaa gaagcgttgt ctgtaaaagg tgcaaaactt
    52141 tggcgatgtt ccgggcaggc acccagccgt tttatggctt ccagatgctg tcgggttcca
    52201 tagcctttgt gccgggcaaa gccgtagccc gggtagcact cgtctatctc aagcataaat
    52261 ctgtcacggg taactttcgc cacaatgctg gccgctgcaa tactcaagga acgtgcatcg
    52321 cctttaatta cctgcatggc gggctgctga aagccggaca gtttatagcc gtctaccaga
    52381 acaaaatcag gagagactga taatgccgtt acagcctgcc gcatagcaga ctgatttgct
    52441 gcctgaatgc cgaagaggtc tatttgtttt gacgggattg tcgatacgga aacaatatga
    52501 ccgcctctca taatttcctc atagagttgt gctcgctttc tttccgttaa gcgttttgaa
    52561 tcattcagcc cgcagacagg agacttcaat ataaccgctg ctgccacgat aggaccggcc
    52621 agaggaccac gcccggcttc atctactccg gcgatatgag aaaaaccctg ctgccaaagc
    52681 gcatcttcga tcgacagcat cgccgagagc cgttcacgtt ctgaaggggc tgtatcgctg
    52741 gaaagagttg ctaaaaaaga tgttaaatcc ggatatggtt ccatgaacca acactgtatc
    52801 tgatttgccc ggagatatgc aatttaaacc gggcgaaaga ggaaagggcg cacaaggaag
    52861 gctgtctgcc gatgaccctg cctttttatc tcatatgaag cagtactttc ataacatccg
    52921 gacatttcgc acgactgaaa gcccggacgc cgtccgaaag tgcgtaggct tcactgatca
    52981 tagggcgtac ttctactgtt cccagtgcca gcgcttccag agcagggcgg aacgggccgc
    53041 accgggaccc tagaatcttc acttcattca cgaccgcact gaccgagctg agcggggagc
    53101 cttcataaca ggtcgttttc aggattacag agccttcggg gcggattagc tgcaaggcat
    53161 gcgtcagtcc atcggatttt cccgtggctt ccactacaat gtcagccgat tctcccttat
    53221 atttttcaaa aggaacggca gtgattccga agtttctcag caattccatc ttccacggat
    53281 gctttccaag acaaatggta ttctttgaat aaagccacaa tgtctgggtg atcagctgtg
    53341 ccagctttcc atcacccaat acaagaattt tatcttccga agagggggag agctgttcga
    53401 caattctgaa agccgccgcc agcggttcgg tgaatgtagc ggtttcatcc tgaatggaat
    53461 ccggtaccag atgcagattt tcttcgggaa ggcatagata ttcggcgaaa gcgccatgcc
    53521 gtccttgaat accgagaaca gttctttcaa cacagtgtct ggtcatttcc atccggcaat
    53581 agcgacagtt tctgcagggg cagttgattt caccgacaac gcgttttccc cgtaaaaaag
    53641 ggttgtcaca ttccaccacc agaccgataa attcatgccc caaaattcca ttgaaaccca
    53701 tgtaaccttg tgtaatttcc aggtcggtgt tgcagatacc ggccgccagc acctgaataa
    53761 gagcctctcc acgtgcagga actggatcag gcatttcctt gattgagaga gaaccgtcaa
    53821 agtacaaggc tttcatttgt tgaaccctta tggtgatttg atcattttga agcagaagtg
    53881 ataattttct ttgcgccatt atgcatttca tcgattgatt aaacaaaatt tgaagcctgg
    53941 cttctttcgt aaagggcggc tattttcggc ttgtttcagg ctgtttcggg ttcaaaagaa
    54001 aaatagcaga aaaaaatttt cagcccgact tgaagatgtg ctaatattat ttgtataaag
    54061 ccaattaaac gatgaaaaaa ctgaggaagt aaaagaagca tcgccatgat gcctggaatt
    54121 gaagcatggg aagtaaaggg ctgtgatgtc caaaactatt tgtcctgttt gctttactga
    54181 aaatgccgaa gaagccgagc ggtgtcatca ttgtggcgcc ttgattctct ccggcagcgg
    54241 tacgactccg catgaattat cgtccgatgc agaaaacagc atggactaca aaagggggga
    54301 tttgatagca ggcaggtacg aagtgcatga cctgatcggt cgtggcggca tgggggcagt
    54361 atacaaagtt catgacaagg tgctggacga agaagtggct ttaaaagtgc tgcttccgga
    54421 atttgtcgca gacaagcagg ttgttgaacg tttcatgaat gaagtgcgca tttccagaaa
    54481 aatagcgcac cccaatattg tgcgtattca tgatataggc catgctgaaa aaaccatatt
    54541 tatttccatg gaatatgtgg atggcagttc gctgcggtca attctggata aacgcggtaa
    54601 agattctcat atgtctatcc gccagagcat ctacattatt tctcagttgt gcaacgcctt
    54661 aaaatacgcg caccgctata tggttcatcg ggatattaag cccgataaca tcatggtcac
    54721 gagaaataac catatcaaat tgatggattt cggtatatcc aaactgaaag acaaccgctt
    54781 tgaaagtcat gcgggggtta ttgtgggcac gccttattat atggcgccgg agcaaattca
    54841 taatgccccg gatgtggatg gccgggcaga tatttatagt ctcggggttg ttctgtacga
    54901 gctgttgacc ggaacccttc caaccggtat gcggcattcg atttcacagg attataatca
    54961 tattcctgtc gaactcgagg tgattatctt tcgttgtctt gacccggata gagaacagcg
    55021 ctttcaaaat ccgggcgact tgagagatgc tcttatctca ttgaatgaca gcctgataga
    55081 taactccact ccggggatcc agtccagtca tttttcgaaa gcagataccg gagcctctaa
    55141 tttgacgacc agtattctgg catcggcaat ggaatctttt ctgcgtgatc aaggcggtga
    55201 ctccgcaagc gatgcagaaa aagattccgg tctgtcttcc agaataaaga cgcccgcaga
    55261 agagtctgca agtggacttc cggcttattt tgagacggat tcgccaagat cgccgtcaac
    55321 tataagaaaa aagaaagctg ttgaacagtc cttttttcag cagtacaagt ttctactgat
    55381 cggcttcgct gtacttgtgt tggtcatcgc tttatttgtt gtgcgaaatc gggtgtggga
    55441 aatttccgaa aacttgggaa tcgttgctca ggagacaatg caggtagatc ccggggagca
    55501 ggcagagcga ctgctggcag cggcagataa atttcaagtc ctgatagatg tatcgaatgc
    55561 ttacaagaaa aataagacgc cggaagggcg tgccttgctt gaagcagtgc gggattattt
    55621 tgtagagact attgaagcac gggcatacgc gcgacctttc agcatggata aactgaatga
    55681 ggcatcgctg gaagctgctc aggcggcgca gatagatggt ggatcgcgta tggtcgaatt
    55741 cgcggacaga atgaccagag aagccgttta tttcaagttt gtcctcaccc gttttgacag
    55801 cactgcgggc acagctgaat ttcgtttgaa taacccttat attcctgaag aaacagccac
    55861 cgtagccgtg ggtgaccttc tgctggatcg ttttttagtt gtgagcataa caagccaggc
    55921 cgtacgtttg gaggatacag atcccaagtg cgccggacgg cctctggttg cccgattaat
    55981 ggagcgcgtt tctgaccggt aaatttggct tcctggaaaa gagataacga aacgcatctc
    56041 ctaaaatcag ataaagaacc ggaaaagaaa tgaaaccggt cttttgggtg ttctctgttg
    56101 tgggagtgga gtgtatttta cctgcctgac cgatgcaagc ccatggacat accaacaaag
    56161 ggtaaagaat tatgaagcta tttctcagaa tgtcgatgct tgtgctgctg cttatccctc
    56221 tcacctccgg atgcgcacgg gacagaaatc aatccgggat ttcgacggag accagcgggt
    56281 tcagcagtgc cgaggagggc gcaaaaaaag agttggagct gcttattaaa aatcatatcc
    56341 aaacgagcaa aacgtacaat gatgcggcgc aggtgcccgt ggtgtaccgc cgtccttatt
    56401 atttgaaaga atactcagtg tattttaacg gccctgatgc ctttgacatt gcttatagag
    56461 aacttgaatc aaaaacccgg ccattaatgg cagaggtcac cattgaaaag gttcgttttt
    56521 cgacgaaaat gcaccgaaag aaaaatctgg cgcgggaaga ccatgagttt gtccgggata
    56581 ccggggttga aaaaatggtt tatgaattgc ataatggcag atggacacgc atcggtgcta
    56641 tttttgatgc aaaaaccacg gaagaacagg taaacggcga atggattccc aaacgggagg
    56701 aactggaaag agtgttgccg gaagagcagc agcccggatg gttcggcagg ctgtggaatc
    56761 gcatccgagg ggaagaataa ccggctgaaa gagagaatag catgactgaa gtgcccggga
    56821 aagacgaggc agcaattggc gtgcagggta aaaactggct gcatgaaatg ccggttgtgc
    56881 ttgcgttgct tctggttctg ctgccgcttt ttgggggagg gaccggtatt gtcgtgggag
    56941 aaaccgtaaa cagcaggctt gctactgtgt atgctttgat acatgatggt gtctggcata
    57001 tagaccgccc tctcagtgaa gatcccaatc cttttgaaaa caagactgtt gataaagttc
    57061 agcgccatga cggccactat ctctcaagca agccgccggt gcttccgctg ctgatggctg
    57121 gagaatatgg agcgttacac cggcttttcg gctgggaact gaccaacacg gatcaactgc
    57181 gacatgtgct gcgcattatg attttcacgc tcatgaagct gccacatatc attggggttc
    57241 tgtgctttac tctgctgctc cgtctgtttt tgcccaatcg ttttcatgtc gcttttctga
    57301 ttctggcgct tggtctggca agccccttaa ccggttttgc ctgccagctc aacaaccaca
    57361 cgccggcagc agctggagtg atggtttgcc tctatctggg tatcgggctt tatactgccc
    57421 ggctgctgcc gactgcctgg cgttttcttc ttttcggatt tgcctgcgct tttgttttcg
    57481 tcatggatat gcctgttact attttcccgg cggtacttgg tcttctcctg ctgcagcgtt
    57541 ttccgaagca aagtgtctta tgggcgggag cgggtgcact tcccttgttg cttcttcatt
    57601 ttgcgcttat ggtggtgatt accggaaatc cgttgcctgt gcagactcag acttccatgt
    57661 acacctttcg aaattcctac tggcgaaatc ctatcggcgt ggacgggctg aatgaattct
    57721 ggtttgtcta tctttttcat atgaccttcg ggcggttcgg cgtttttctc cttttcccga
    57781 ttacgctgct tgcttttccc ggtgtttata aaaagatcag agaaccgcag aatctgctgt
    57841 cccttcctta ttttgctctt ctcaccgctt ttcttgtcct ctcggtttat tatgtgctct
    57901 ttaccaacaa ttatggcggg gcagcctacg gctttcgctg gtataatggc gcgatccctg
    57961 ttctcctcct tctgtcactg cctgccgttt ctgctgtcag aaactggaag tgcgcctttc
    58021 tgctcgtgcc gctttttgtt gtttccgcct attctgcctg ggaatgcttt caggcgccct
    58081 ggggtgcgag tcatgagtgg acttgccggc tgctcttcgg gcctgttttt taattattca
    58141 gaagcatctg aatacgccat tcatcttccg gcgggggaga cgggctggca aagttacggc
    58201 gagccatgaa ttttgccgct gtttttacgg catcaatact tctgacagtc cagcctgtgg
    58261 aggcgcgttt cacggactca tgaagcacat ccagcgaaga gtaggcaccg gatccatgca
    58321 cttttccgaa atggagatag agggatgctc cgggcctgca gaatgatgcc gcttttttcc
    58381 acacctcccc gagatcattt atatagtccg tgagggactg atgatgcaat tcctggcttt
    58441 gtgaagcact tgcttcatcg tcgcccagca gccaggaacg aagccacagg ccgcagtttt
    58501 tattcggcag cccgtaataa gggggagagg taattatcag atcgaatttg ttttcagctg
    58561 caggggcttc tgcacggaga aaactgttct cctgcacgac acctgacccg gatgcggcat
    58621 ccagtattgg taactgccgt gctttttcag cgataagtct ctgaagagaa tacggaggcg
    58681 gttttgagcc acgtgcttgc caatgagcag acagcaaatc ctgtggcggt gcaaaagtgg
    58741 caggcatgtc attggatagg tacttaccct tttgaagagg gccatgcaga atacccagaa
    58801 caattccata gagtctggct tccgcctgat ccaggttacc ctgaagatag cgttcacgca
    58861 ggcgcagcag cccttcatat acggaagaat gaaaacaaag attccagaag ccgttctgct
    58921 tattctgagt attcacgggc gttgtttcta aaatctgttc tgcttttgct atgacctctt
    58981 cggccgtggc agaggcaagt tttgctctgg agacagcaca ggctactgga tgtatatcca
    59041 ttccactgac aaaatgtccg gtaacagagg ctgccagaag agttgttccg catccgcaga
    59101 aagggtcaaa aactcgtttg ggcgggtatg tattttccag aatgttcagc ggaaaggaca
    59161 gaggaaaacg ggtaaaaaaa gggcataagt gatcaagagg tgtgtacggc ttcagcggtt
    59221 taccaggcat ggggcgcaaa agaaacttcc cggtttatat cagtcggctg tttatgaatg
    59281 cttccagaag gaggggacaa acaagaccag aatagcataa agttccaggc ggcccatacc
    59341 catcaggaaa aagagcagcc atttggcaga cacaggcaaa aagccgtaat tgcctgtagc
    59401 gcccacgagc tccagtcccg gtcccacatt gttcagacac gcggcgacgg cagtgaacga
    59461 ggtgaccaag gacaggccaa gtccagacag ataaagggtg ctgagggcgg cgatagccat
    59521 gtaaatgaag aaaaaagcaa agatggactg ctgcatatcc ggttgtataa ctgtttcttc
    59581 aacacggagg gagtggacgt tcttgggttt gaaacttctt tcgatctgcc agtaggccat
    59641 ttttaagaaa agaataatcc ggattacttt cagcccgccc gctgtggagc cagaacagcc
    59701 gccaataaac atgatagcga tcaggattcc ctgcgacaga ggtggccaga gattaaaatc
    59761 ctctgtgcag aagccggttg tagtgatgat ggaagcgacc tgaaacagcg ccatccgcat
    59821 cagctcaatg ccctgaggaa gaatgccgcg atgctccggc cggataatgg tctgtccgtt
    59881 gagttcagcc tgataaagat taacggttat caggagtgtg gaagcggtaa cgatgagcag
    59941 aaaaatccgc cattccgggc tcgcaaatgc tttcttgtgc tgtcggtgaa taatttgatg
    60001 cagtgttccg aaattggttg cggaaatcag cataaaggcg acgagtgcca tctcgatggc
    60061 aaaactgttg aagtgggcga cgcttgcctg ataaggggaa aagccacccg tggacagggc
    60121 gccgaaagtg tgggtgaggg catcaaaaag attcatccca agaagacaga gaatcagggt
    60181 catgactaca gtcataccgc aatacagctt cagaagaatc aatgccgagt ctttgattct
    60241 cggtcgcaat ccacttttaa cgattccggg cacttctgaa cggaagagca gttttcctcc
    60301 ggcgcccagg aagggcagta cgattaggag gagcagtacg attccaagcc cgccgagcca
    60361 gtgggtaaaa tcgcgccaga aaagaaggct ccggggcagt ccttctatag cgacgagaat
    60421 ggtcgctcct gtggttgtaa aacccgacat gctttcaaaa caggcgtctt caaaagtcag
    60481 atgccctgag aaatagtagg ggagacctcc gaaaacggcc cctataagcc agccaaggcc
    60541 cacaagcgcc aatgtctcac ggtgaaaaag ccggtcattt gattttttcc caaccaagta
    60601 gagcagtccg cccaggctga atgtcagcat cattgaaaag aaaaaagcag gaagcatctg
    60661 gtcttcccga taaatgaaag cgcagagaac agccggcaca aacattccgg tggcgaccag
    60721 gcagaaaagc cccatgtttt ttaacagtgc aagataattc atgagatctg ttcattcctg
    60781 tgaaagtcag atcaatgatc cggcttctat ggcattatac ttgaaagaga ggcggaagta
    60841 ctgaaaaatg gcgggcggga acgccgaaaa agacaggcta tacaagggct gttaagatgt
    60901 tgccgagaag atcactctta gcggtgaaaa agtttttgta tttcgtgtat gttttcagtg
    60961 gtggcaatca gcaccaaaga atcatcttcc tggatcattg tagtgccatc cggtataaag
    61021 gcttcgttgc cgcgcatcac cgttgcgatg agtacgcccg gcggcagttt tcctttagag
    61081 tctttcagaa ggagattttt cagaggactt gttgcgctga tggaaatttc cagaatttcc
    61141 gcctctcctt cattcagggt cgttgaagcg gcaattgtct tctgacgaac aatcttcaac
    61201 acactatttg ccacaacact tcgtggtgta acggcaagag taactcccag tttttcggcg
    61261 aggggcgcaa aatccggatg gtgaattact gctgccacac gcgatacacc gacttcccgt
    61321 gccagtacac cggctacaat attacgctcg tcatcttctg tggtggcaat aaaaagatcg
    61381 aaagtttcaa gatgctcctg ctccatagag tcgcgggtag ttgcatcacg gttgattacc
    61441 tgaatgttgc gttttctgaa ttctccggca acggcaagag cgcggctgct gttccgttcg
    61501 aagagtttta cctcagccac tgtgctttca agagtatggg cgatccagag cccaatagtt
    61561 cctgcgccca taattgccac gcgctggata gccgaaacac ggccctgaaa tttactcagc
    61621 gcatctgaaa gtttttctcg tttgctcagc aaagtggctt tgtcacctga cagaatacgg
    61681 gtggtgccgt ccggcacaaa gacttttcct tgacgttcaa tcactccaac cagggtgccg
    61741 ctgcccggct tcagaatctc acttaataaa tgacccgcag cgggagcgtc cggcgaaacc
    61801 tgtacttgcc tcagcaagat gtgcccgtga gaaaattctt ctgaaactaa aacgccgggg
    61861 tggagtatgt agttggaaat ttcctgcgcc actaacgcat cggggctgag tatgtaatca
    61921 atattcagca attggtcgta catgtaactt tcaaccatgt acggggcttt ttccacccgg
    61981 gcgacagcct ggcgtgcccc gagacctttt gcggcagctg cagcaagcag gttggtctca
    62041 tcgtgaccgg tgcaggcgac aaaaagatcg gcttcgccaa catccagaga ttgcaggaac
    62101 agggcagatg agccatcgcc gagaataacc tgagcattca gcatctgatc gatatattcc
    62161 tgacgctccg gatcattttc aatgaccgtt acctcataca cctcttcact gagcagcagt
    62221 gccagatgat atccgacgcg accaccgccc agaataaata tgttcatgac atgctccaga
    62281 ctgctttccg acacgtttaa gagtttttat aagagtacac gacttcaccg ggacgaaaaa
    62341 caaatgaaaa gagattttta aaaattgtta cctaaaagaa cactagcgac acagatcttt
    62401 aagaagtatc catatcgcca gtgtattttc agataaggtg tgtccggaac actattccat
    62461 ggaaagtgaa tcggtggggc aggcatcaac acacgcagcg cagtcgatgc aggtgtcatt
    62521 gtccactacc gctttttcgt tttcgattac gatggattca gtcggacaag tgtccacgca
    62581 ttcagcgcag ccgtcgcagg tatcaacgtt tacatttacg cccatggtat caactcctta
    62641 aaatgtttct tttcatatac acgggtatcc agtaacatct aatgaacacc ccttacacct
    62701 gaccccatta tcccaaaaca ccggtagtaa aatccatttt tataccgcat ccgggaaaag
    62761 aggtccggcc aacatcaaaa ctgagtggtt tgaggcggtt ttttcataat aacactatta
    62821 tcaccttttc agccggggcg catgggctgg ttttagcacc gaacacatgc cggaaagtct
    62881 tttaaaaaat actgctgtaa cagtttattt ttttgacttt cagctgatga gttaaacttt
    62941 ctcaatacat atatggaaac aaccgggact tgggatttct tccttttttc tgctataata
    63001 aggcgggtat gactgctttt cagtgggcag aatggcgata ccttcccttt ttcataaaca
    63061 ccgcttccaa aggcaaatga ggggcatccg ctagaaaggc ttgattgtgt ttaatatact
    63121 gcgctacttc agtgggcttt tttcccatga catggcaatt gatctgggca cagccaatac
    63181 tcttgtttat gttaaaggtc agggcattgt gctgagtgag ccctcggtag ttgccatcaa
    63241 taaggatacc ggcaaagccc gtgctgtcgg cagcgaggca aaaagcatga tcgggcggac
    63301 tccgggcaac attattgccg ttcgccccat gaaagacggc gtgattgccg atttcacggt
    63361 caccgagcaa atgc
//
LOCUS       DAOPWB010000004        55210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_540_length_55210_cov_5.283274, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000004 DAOPWB010000000
VERSION     DAOPWB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 55210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55210
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_540_length_55210_cov_5.283274"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1204)
                     /locus_tag="PKN92_00935"
     CDS             complement(224..1204)
                     /locus_tag="PKN92_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ47384.1"
                     /translation="MNKVVLMTAVLSLSLGAFAENEQKIPLKFRDNGTFRIVQFADLH
                     WTYGYGEDKLTAKLIRTVLNEEDPDLVVFSGDNITGGTLLPNRSLRQVTSFCAERKIP
                     WAAVFGNHDDEGNASRERLMSIMSGLPYSLSVAGPEDIDGVSNYSLVIGSSKNPEKPA
                     ANIFLIDSLSYMIWEGQSRYAHIKESQLEWFKQESTRRREAAGGAALPSLAFFHIPLP
                     EFRKAWESGNAIGVKQEDICDSPVNSGLFDVFKETGDVVGIFCGHDHTNDFIGEVDGI
                     YLGYGRGGGHGTYGKAGFPRGGRVIELIEGERKFRTWHRLETGEQVMQIP"
     gene            complement(1319..1822)
                     /locus_tag="PKN92_00940"
     CDS             complement(1319..1822)
                     /locus_tag="PKN92_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNZ47385.1"
                     /translation="MHEKSIDLLNRAIADELTAVHQYMYFHFRCDDQSLDMLANLFRR
                     IAIEEMLHIETLAERIFFLGGEVELAASAEVQKIADVKEMLKLASHLEEQSVRDYNNF
                     AIECGSHADSVSKKLFEKLVEDEERHFDLFDTELGHLKRFGDQYLALQSIERTKVIGA
                     ENEPPEA"
     gene            complement(1959..2783)
                     /locus_tag="PKN92_00945"
     CDS             complement(1959..2783)
                     /locus_tag="PKN92_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNZ47386.1"
                     /translation="MRSFLTTTAVFLLMSFMLCLAADVAGNTAEPQPEPLGLYALAAA
                     YAPYCRICEEEKALCWDDGTVLYYDDGIEKESFEVLLDQASLKDQMAQPYPRHWRDGA
                     PGVNEDPGRIRNESFFRKIYGESADAVRAQLTDVPWLNGTVISFTRVNGADEALARVR
                     EKMMQLPPAAQRYIMNPSGSFNWREIAGTRRLSMHSFGIAVDFQLPPRLYRYWLWDQK
                     GTQEGSVYPEGILSDDRLRCIVEIFESEGFIWGGKWHHYDTMHFEYRPELLIQEKR"
     gene            complement(2791..3681)
                     /locus_tag="PKN92_00950"
     CDS             complement(2791..3681)
                     /locus_tag="PKN92_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ47387.1"
                     /translation="MDTQQKRSRREFIGQTIAAGTALGLMPAVARFVQAEEEKVAPKI
                     SFVTANLVAQVTGFRFKLENWGDQHNKTVAATDAAAWEKICKEIAGLGFTAVEVWEAH
                     AAPESMDREKGRVWKAILDDHGLKAIGYGGHLSSETVEICHWLDIPQINGGIGKNTPE
                     EATALCERSGIRFNTENHPEKTADEILKAVGGGNEWLGVCIDTGWLGTQGAAGPQIIR
                     ECGAFVRHTHIKDVEAPGAHLTCLLGDGTAQVEACMAALAEIGYDGWYSWEDEPEDRN
                     PLLSARRNYEWILSRIGQKS"
     gene            3808..7491
                     /locus_tag="PKN92_00955"
     CDS             3808..7491
                     /locus_tag="PKN92_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47388.1"
                     /translation="MLLKYQFSFIAVLLLLSSGTYAEPYRIALFHEPGMPAPAITPEP
                     DFWENMVAGLPGFQMERRGLKDLNNPTHFSRDHFDLLLIPTGASFPAAAADALISFLH
                     KGGDLLTSGGYAFDALVIEDQGAWKTQTDFLENAASIARNPEHSAVPGGDFESPVSCW
                     ISHNPAYAGITTEVSYTGAACAYALSSLDGGTGRWECVLGCQPGNHYLIGAHLKTDAL
                     LGRGYAYLAVYQYDREGAILDFTDFAQVGKASDWMRHEAAVTIHPQTDRTVFQAGLHH
                     IQGTMWFDSVTCAAIPEEIRINAHYGKPGDGLVVRKEQLQIFSPDRRFTSSADAYAFY
                     AQTESGRNSEPPPALHGFEATAQLSGNGHISPLATFATPEEYLPCPGTLVFNHQGFFK
                     DSHWALFGVDNLNFPALAPDAYREALLRLRAGVTISELRPAYVSYQPGESGSLSASVR
                     NTSKEKRVLRVSLAVDDLFCAALTDRPEQEVALAPGEAVTTSWAFTLPESDSLPALCS
                     IVLRVYEGTLLCDERTTGFVVNRLNRLPAACRKAPESCRFGTDLWSSVFLSPSHSPVS
                     WSEDLKLMKQSGLTVAEMLQLCPADYEYTEEHWRFFDGFIQLCADQRLTYMAGLLIGK
                     NVCIDNDTLKKQQALCRSFAERYKNVPDLIYYLNGDFRLDLADTEDIRALWNQFLATR
                     CADAASLNEAWKSEGFPAPGAVPVQEYAAQGWYDLHARDLNEFKALLMNRWIQALIVA
                     IREEDPHHPITSEYYQHPFSGIDLRNSLGGQYASNFGYFDRPNLDRARFAATARMYDM
                     RREGKALHVGEFGVKTHDAWAEERGGTHYHIRRSEEEQLRFFWDIVHHAWALGFSQIQ
                     NWCWRDDPDRIFPWGVVWANPSRPKPVLSLWSRLAGMNRMITVQHEPADIVFVLSDGW
                     RSGAPESFSLEVMLNALDCLLAANIPFDVVSENALTTLQDNLPKAIIFPAAYALSDET
                     LSLMQGYAIRGAAVLLSGDPGINPQGQRNQERYRQFLGSTFLAETGDAKGLPVPRIKT
                     ETATLCLTEGTVTLYAIPCGAGAIYWSPLLWESAVSADLFGKDRHLTADPRQNLYLPF
                     LKMLGFSSPLPVSNTSGIWRTSLVPSGENRLISLFPVTGENTPGTVSVAYDGLQFQFE
                     YTYAAPSALLLNPENKILALSGCGSLSVNGIPVVQSKGMWLIYSDEMLPLEACSRVHA
                     FSDREAVFFPGDNRSLNCMQFEQ"
     gene            7576..8544
                     /locus_tag="PKN92_00960"
     CDS             7576..8544
                     /locus_tag="PKN92_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ47389.1"
                     /translation="MQSSRRHFLAGSLLAGAVVLAKSSPLTAAAASADAPWKCGVGLN
                     GFMSSGDHFKKTYPLWEILDYAAQAGFNGVELVEGWPMGGYPDYEEEKRVAALRRLYN
                     QYGLQIYTMQTGGADSYASSAEARREWLARFRRLATLCKALGGDFIGHWPGGGLEGNK
                     TVDEAIKHLGSSYREASKICADLGMHMSFEIEPPFLFNTYEHLIGILEAADHPACRTN
                     YDPSHFDVMSGSKGKPEDMLKRLGVQHIGHVHLTDTDGTQFNGTSTHLPCGEGHCDIQ
                     ASLHLLHEGGYKGWIMIDAWMTEDAYYTTQKGIDAVRVAQQAFSAE"
     gene            complement(8557..9708)
                     /locus_tag="PKN92_00965"
     CDS             complement(8557..9708)
                     /locus_tag="PKN92_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ47390.1"
                     /translation="MNRYTAVQSLLILFAIALLISPPVIFAESIEKLDPNMAVADPDG
                     EWIWYDALLLGIEGKVWGETEKPYDRLPTKAEGVVTDSVWKLSKHSAGMAVHFVSDAE
                     KISAKWEVTNDNLAMPHMPATGVSGLDLYVRLDKGWHWIGNGRPSQKETQAVLASGIP
                     AGSHEYLLYLPLYNGTASLAIGVPKEASLAKAPARPEGKQKPLVFYGTSITHGGCASR
                     PGMAYPAILGRRFDRPVVNLGFSGNGKMEAAMGELLQEIDAAAYILDCIPNMTPELIA
                     ERVVPLVKALRAARPGIPILLVENIQYQSGAFLEASRIAYEEKNEALQESYGTLLGSE
                     YISDLYYLPGDTLLGSDGEATVDGVHPTDLGFQRMADAFEPVLKSFLSE"
     gene            complement(9736..11070)
                     /locus_tag="PKN92_00970"
     CDS             complement(9736..11070)
                     /locus_tag="PKN92_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47391.1"
                     /translation="MSRKISRRSFITRSAACAVAVPLILSRKTPASASTQVLNHAAIG
                     VTGQGTSDLEQIFSSGKVHVRALCDIDANNLEKAAVRYPGAKLYRDWREMLAKEGEYL
                     DSINIATPDHIHAPSAMAAIRKKLHVFCEKPLTHEVYEARRLTEAAKEYGVATQMGIQ
                     IHSHDYYRTAVHWVKEGAIGKVKNWYSWSAAVYTTDDKKRPAHRDPVPAHVDWDLWVG
                     VAPMRPYVREVYHPFYWRCWRDFGGGATGDFGCHIFDPVFTALNIGAPLTIECDAECY
                     SEEVYPAWTIANYLFNGSDLTVGKTIEAVWMDGGKMPEKSVSPHLSEAYQLPKSGSLL
                     IGEEGSLVIPHVGPPKLFPEEKFANYPAPELEPMNHYHDFVEAALGNRTAGAHFGFSG
                     PMTEAVLLANIANRFIGEKLEWDAGALKFTNNKKANRYVKRRYRTGWHVKGL"
     gene            11369..12931
                     /locus_tag="PKN92_00975"
     CDS             11369..12931
                     /locus_tag="PKN92_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNZ47392.1"
                     /translation="MENIIGLSNIDVSIIFVYMLGTLLLGLFCTRYIGNAEDFFISGK
                     ALPFWAIGFSIVVSDIGATDFVAVAGATFRHGVSAANFDWMGSMPAMVFAAFVFVPYF
                     WRTGVFTIPEFLGRRYNAGVQFLNAAIWAVVLFLMLAIMLWVTADKLMHTVLGWNAKW
                     SVWLMAIVTGLYTFSGGLTAVVFTDVVQLVVMYVGGFGLLALSLWEVGGWSALREQVL
                     AKGPEFQQHFTLLLEHDTTGPFPWTGIIFGLGIVLAIAYMSGNQVIVQRTLGARTEWD
                     AKGGMLLGGFLKSMIPLMVALPGLCALILVPHLPVEEADRAVPEMIRLLLPTGLRGLM
                     FAALFAALMSSISGTLNSASTIFITDIWGQGLRLFKCKPLSEKGALNMGRGFTAFLII
                     TTAILAKPIADRESIYVFTQTILSMFQGPTLAILLLGITWPRATQWGGLAGLALGVIF
                     CFILNYTPGLFTSSEPFLFVAWWSFVFSLVVTVVVSLLTPAEPEEKLRGLTWGSVVHS
                     SEAQAALEERNS"
     gene            12931..13215
                     /locus_tag="PKN92_00980"
     CDS             12931..13215
                     /locus_tag="PKN92_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47393.1"
                     /translation="MHLETLLRKYCAIIFYPILSKIFHPINHLLAPIYKPWATIAAMS
                     FFVGTMIWVGVILKESYVNEGRPNKSIWTDLRLWTVVSMLPHVFVYWYFR"
     gene            13225..13530
                     /locus_tag="PKN92_00985"
     CDS             13225..13530
                     /locus_tag="PKN92_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47394.1"
                     /translation="MNNTPDTIESDAKVSKDTCCSCEGTAPADDPKCMKKRDGETNIA
                     VGLFLFALGIPVLLGTLGIEGNVRAATVNAVCGLTLLSIGGGWVAYGWRQFKQATRR"
     gene            13537..14118
                     /locus_tag="PKN92_00990"
     CDS             13537..14118
                     /locus_tag="PKN92_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47395.1"
                     /translation="MTGLFLPLRIPRDEARRLFYRTGIRNLYGLIRRRPAKMGTDGLP
                     ASVELLWLPAYAVRFALSLGEIQSQGWVSVDANFGGFALFERVDILEEREEKGECLPV
                     ILSPDEADKKGRHGLLRYFLRRRGSKPLIGKTEDSLLYYAPVWVYYYRRGSKIDLKVL
                     DGYTGSPMGGQVRTAIVNAFIAQRKRKQQEQPS"
     gene            complement(14210..15544)
                     /locus_tag="PKN92_00995"
     CDS             complement(14210..15544)
                     /locus_tag="PKN92_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47396.1"
                     /translation="MSITRRRFLTQLSVLGAAPFVLPSRIWSAAHKPNDRINIAVIGA
                     GKQGRYLLEAFMNHKRARVVAVCDVDTTRRLDIQRMVNEFYTGHPEAGSPDCAAYNDF
                     RAVINRRDIDAVCVTTPDHWHAYIAIAAMRSGKDVYCEKPLAHNVHEIKEMMKTVALT
                     GRVLQTGSMQRSMKEFRIACELVRNGMIGKISRVECSFGGPAVPCDLPEETLEPGLDW
                     DMWLGPAPLRPYSSVLSPRGIHKHFPDWREYREYGGGMVTDWGAHHMDIAQWGLDMDK
                     SGPVEIIPPDNPEAVDGGILIYDNGIRVIHQKGFGVHFYGSDGEVKVNRGKFEFWLQG
                     KKIAGFAGKGDEGSLGAALSKVEEEFLKDPKIKLYVSVDHVRNFLDCMESRKKPITDV
                     EVGGRSATVCHLLNQAYYHRQVLKWSPRRMQFARGGGDEAWVTREFRSPWVV"
     gene            complement(15581..16441)
                     /locus_tag="PKN92_01000"
     CDS             complement(15581..16441)
                     /locus_tag="PKN92_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ47397.1"
                     /translation="MKKQSAEQIGVCSWSLQATGPEDLAEKVNALGLKKVQMGLTPHR
                     GDVGVWDNVQEILAASGISIVSGMYSTVGEDYTTPATIQVTGGVVPDQHWEENQELAK
                     VTAALAEKMGLTSANAHAGFLPHDKNDPAYDKLCGRVETLAKIFKTHGVTLMMETGQE
                     SAESLLAFLEEMKKRGVDNVSVNFDPANMILYDMDEPIEALRRLVPYVQQVHVKDARR
                     TKVKGDWGEEVVVGTGEVDWVEFVRILAEAGFTGDYIFEREAGADRVGDIAKGIEHLK
                     KAMDEVSLAF"
     gene            16674..18284
                     /locus_tag="PKN92_01005"
     CDS             16674..18284
                     /locus_tag="PKN92_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47398.1"
                     /translation="MNTAGIISRRTFIKTGALLGAGLYAAGNSDFALSASKNNRRFHA
                     SLSIDALERYPDLLPTVVNAGVDAVWVAAFLQGSWQYPPETVGVWKKRIEAAGLEVHH
                     ITVPLGHPSFTEAAPDYMGELSYQPWQRGILPDGRACYGVCIHPPACDVNAEGVKILH
                     DAAPGMIFLDDDFRLAPSPHDIGGCFCPAHKAAFLEKHGYSENTWEEMLAAVKDRRLT
                     APLRNWLEDACTELSDCFKAQQAMLPEKSLGIMVMYMGSEKAGIRLDDYRDVPFRVGE
                     LQFNDQAFSRVKGKTDELFSVLFHRRFTLPENSYSESTAWPPDGLSPENLAAKLAIST
                     IADVRNTMLMSGLEPYPLFYWDTLAPAMKKHRALHETLAGHKPTGPLKHYWGDAGRWV
                     GDSKPYSLFLATGVPFEVTEIPADDGFTFLGDGDAKALQDGRLKPGKGLCITREKAEA
                     PADVLRYIPEELDALFAFKRELLEKNRDFPYVVEDLPVICAWYPTANLAALWNLSENA
                     QPLTLAYQGREYRVNASGLELVPVPLNA"
     gene            complement(18298..20001)
                     /gene="ilvD"
                     /locus_tag="PKN92_01010"
     CDS             complement(18298..20001)
                     /gene="ilvD"
                     /locus_tag="PKN92_01010"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNZ47399.1"
                     /translation="MSIKNPSGHRQFSKSVTESSERAPSRAMLRAVGFSDEDFKKSQV
                     GIASTWSMVTPCNMHIDALAREAEAGVNEAGGKGVIFGTITISDGISMGTEGMKYSLV
                     SREVIADSIETVVGCEQFDGLVAIGGCDKNMPGAVMAMIRLNRPSVFVYGGTIKPGCV
                     NGCAADIVSVFEAVGRHARGEITDAELLDIEKKAIPGPGACGGMYTANTMACAIETMG
                     LSLPNSSAQAAVSPEKNEDCRRAGAAVMECIKRNLRPKDILTKKSFENAITVVMALGG
                     STNAVLHLLAIAHTAGIKLTLDDFTRIGKKTPVLADLKPSGHYVMQSLVDIGGLTPLL
                     KELLKAGLLHGDCMTVTGKTLAQNLRGTKSYPKNQQIIQPLKKPIKPEGHLTILYGNL
                     AEEGAVAKISGHEGLRFTGKARVYDSEEKALANILNGTVKAGDVVVIRYEGPKGGPGM
                     REMLAPTSAVIGRGLGKEVALITDGRFSGGSHGFVVGHITPEAQTGGLIALVKNGDTI
                     TIDAVKHSITLEVDQEEIARRRAAWKAPKPRYTSGVLAKYAALCASASEGAVTDKNLS
                     L"
     gene            20366..22069
                     /locus_tag="PKN92_01015"
     CDS             20366..22069
                     /locus_tag="PKN92_01015"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNZ47400.1"
                     /translation="MNFLQALALRARVYYWIATWNKKDLHYPPPVKDNPKFMTAWEAV
                     KMIPDGATIAISGMAGNQRCSLLLWGIRDMFRAEGSPKDLTVLCLGGQGGRGKVPGTI
                     EELAIEGLNTRLITGHTETFKGQLKLAHEGKIELQCSLIGMITQIFKAQSEGRNYFIA
                     ETGTGTFVDPRTGHGTPVWPPDAEQFVEVLEDGRLKYTLPLIDTAIFSAPAADRKGNI
                     YFKGSSVLGESESIAKAAKYNGGRTLVNVGRMVEEGYDDQFLPAEYVDAVVYWPGTEQ
                     AATIKHKKYWNCLTPHSTMSTEEGLKRVRLVNKTLRVMSTRTPIDDVLARLATKVFAA
                     HARKGDIVDIGIGLPEEVARLLYESGVMKELNMMTEAGAIGGVAAPGVFFGAAVNPRD
                     LIPSVTAFERMYNRLDWVILGMLQADSEGNVNVSKRGEGPLNHVGPGGFIDLVTCANS
                     ILFCTAWGARARFEIKDNKITILNPGKPKFVAKVDEITFNGKEALKHGKKVYYVTQVG
                     AFRLTERGMELFYVMPGIDIQKDIIDACEMKIVLPENGEVPVVGEDVITGKNFQFQLQ
                     Q"
     gene            22171..23877
                     /locus_tag="PKN92_01020"
     CDS             22171..23877
                     /locus_tag="PKN92_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47401.1"
                     /translation="MNLFSKIALYAHVLRWRLMWNRFNTHFPPPDFDNPKFMTAWDAA
                     GLIADGAIVGTSGIGSNQRPSVLYRAIRERFLKTGHPRDLTVVGIGGQGGRGIVPGTV
                     EELGIPGLNTCLITSHTEMFKAQLRLAQENQLELQCSILGAITHILRAQGDEGINYII
                     RDHTGAGTFVDPRTGGGTAICPPDGRQFVEVTEDGKLKYSLPFIDTAVFNADAADRHG
                     NIYFKNSSVICEGFSLPKAVKRHGGKVIVNVGTLVDEGYDTNFVPAEMIDAVVYWPGT
                     EQTGTVKQKKYWDFFTVNSRTSTEDALARVRQINAILKITAKRYPVDDVLARLATRIF
                     AAHAHAGDHVDIGFGLPEEVARLLYESGIMKKLQMINESGAFGGISAPGIFFGASINP
                     DEITTTAETFDRLYKRLDWAILGALQVDSRGNVNVSQRGKGAMNHVGPGGFIDLVTCA
                     KNILFCCAFGEHSDVTVQGAKLRVGNPGKVKFIDKVDEITFNGEEALRQGKKVYYVTH
                     LAAFHLTARGMELTHIMPGIDIQKDIIERSSMRILLPENKEPEIVSEEVVTGKDFSFS
                     LS"
     gene            23914..24948
                     /locus_tag="PKN92_01025"
     CDS             23914..24948
                     /locus_tag="PKN92_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47402.1"
                     /translation="MLFARIHTTLPFTLSLLLLCLYGCSTNSASEKTVRKSSPPAVSA
                     NTHFPRVTLEHSPLPASDAFRLLGEKAGGGFVLMAGLEERPVPAANWQKQPYGDVVNT
                     YAQELDCLLFPASGYYFIAPEGYESLAAVSLSAALPERYRTLTASLTLGAKTKLYTAF
                     ALISQVLGITILSDNFIAESPCGELHLEDLPLGIMLEALLQSARIAPDSMALDASDEY
                     LFIQAKENIQPKDLLLNDRSLPAESSAILDKRLPRFAIPPVSPDNEAALFGAESIPLK
                     DALLPLSTQAGIEIVCRRALADIPINPFVCTDVSLRTALNLLIRQWPLPSFGYEVQDN
                     RILLRERAEP"
     gene            complement(24980..25435)
                     /gene="dut"
                     /locus_tag="PKN92_01030"
     CDS             complement(24980..25435)
                     /gene="dut"
                     /locus_tag="PKN92_01030"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNZ47403.1"
                     /translation="MTSRQIQIQVHRLPGTEDLPMPVYATEFSSGMDLVAAVDSDATL
                     QPGERRAIPTGMTVAIPPGYEAQVRPRSGLAIRHGITMINAPGTIDADYRGEIQVLLI
                     NLGTEPFVIHRGDRIAQLVIAPVVQAVWCPVDVLSETERGEGGFGHTGI"
     gene            complement(25432..26700)
                     /locus_tag="PKN92_01035"
     CDS             complement(25432..26700)
                     /locus_tag="PKN92_01035"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNZ47404.1"
                     /translation="MTKNNFDENIRVVTLPNGFRIAMEHLPYLHSASLGVWIKAGSAS
                     ETQKQSGLAHFLEHLFFKGTKNRNVHEIMDAIESKGGYFNAGTSQEYTTFYVRMLAEH
                     VEIGAEVLGDLLLNSLFCDLEKERNVVLEEIASIEDTPDELASDLLAEFHWPDHSLGR
                     PVSGYAETVAALSRQDLLQFYDAWYKPENMVFAIAGNFDQDRVLAKVEEIFSGLPAGT
                     PESDLRVPRFQAGTKLVERPISQVHVGIATPAPSMGDQERYAGSMLSGIFGGSSTSRL
                     FEKIREEEGLAYNVCAYISSYVPAGMMGIYEAVAPQNCAQALDLTFKELDLIRSSPVS
                     QEELNRCREQLKGSLLMAMESTHSRISRVAKNLLFRNEIIPMEELVAGIEAVTAEGLL
                     DFANRYIRQDACALLLLGPKGTHIPDGIPL"
     gene            complement(26771..29068)
                     /gene="pnp"
                     /locus_tag="PKN92_01040"
     CDS             complement(26771..29068)
                     /gene="pnp"
                     /locus_tag="PKN92_01040"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNZ47405.1"
                     /translation="MLKTASVDVGTEKMTFETGRVAKQAHGAVICRSGDTMVLSAVTV
                     APDMRDGQDFFPLTVDYREKFYAVGQIPGNFFRRESRPSEREILVCRMTDRPLRPLFP
                     KNFLNEVMVCQTVLSADNVNNPDVLSINAASAALHISKIPLLEPVGAVRVGCLDGVLT
                     ANPAMEEMKKSTLDIVVAGTRDSICMVEGQANEVSELFMVEALEFAHGHIRNIIAAID
                     ALREAAGVEKMPVPEDEQPPELVAEIETLAAKRLGDALAIKEKQVRLDTIDALKSEIE
                     ATLLEKYGEEWGATFGAAAGEIFNDLKKRIMRQAVIDTNIRMDGRRLDEIRQITIELD
                     ILPRAHGSVLFTRGETQALVVTTLGTSRDEMRLDELTGDEFRRFFLHYNFPAWSVGEV
                     RRISGPGRREVGHGKLAERALEPMLPFLQEKDEETVQENEIHFPYTVRIVSDITESNG
                     SSSMATVCGGSLSMMAAGVPVTNSVAGIAMGMIKEGNEVRILSDILGDEDHLGDMDFK
                     VCGTSKGITAFQMDTKIKGLSREVMEQALEQARAGRAHILSIMESALAAPREDMSPYA
                     PRIFTITIHPDKIREVIGPGGKVIRQIQAETGAEIEIEDDGTVNIAATNKEDADAAID
                     FIREIVAEVEVGKIYHGRVTRIMNFGAFCTVIGSKEGLVHISELADYRVHNVTDVVNV
                     GDEIDVKVLEIDSMGRVNLSKIAADRELDQVQPAPEGYFEQRGGGEQRDNRGGRGGGD
                     RRDRNDGGRRGGPSRGRRNDRSDRD"
     gene            complement(29209..>29470)
                     /gene="rpsO"
                     /locus_tag="PKN92_01045"
     CDS             complement(29209..>29470)
                     /gene="rpsO"
                     /locus_tag="PKN92_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNZ47406.1"
                     /translation="KKEEKQTLIKEHGGSDKDTGSVEVQVAIFTERINQLNEHLRQHP
                     KDHACRRGLLRLVGKRRNLLTYVANNDVERYRALIAKLGLRK"
     assembly_gap    29471..29480
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29652..30932)
                     /locus_tag="PKN92_01050"
     CDS             complement(29652..30932)
                     /locus_tag="PKN92_01050"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012576012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNZ47407.1"
                     /translation="MNKLRIDEVAHAGKTVLMRVDFNVPQNADGSVRNDMRIRAALKS
                     IQYVRDQGGKLVLMSHLGRPGDVAKAETQEEKDEIQRKNELLKMDPVAAKLSELLGAP
                     VKKLDACVGPEVEAAIAAMNPGDVVLLENTRFYGKKETKNDPEFAQQLAKLGDLYVSD
                     AFGTVHRAHASTEGVAHYLPTSVAGFLVAKEIEYFSKVLDNPERPLVAILGGAKVSDK
                     ITVIENLLNLVDTLLIGGGMAYTFLKAKGYEIGKSILDKDGIDVAKKVLALAEEKGVK
                     LQLPVDIVVADAFADNANTQIVGIDAIPADWEGLDIGPKTIALFCDVIQQAKTVVWNG
                     PLGVFEMPSFVKGTRAVADALAESPGVTSIIGGGDTAAAVIQFGLAEKMSHVSTGGGA
                     SLEMLEGKTLPGLAALSDKPESGSCSGCCSCQKS"
     gene            complement(31020..32018)
                     /gene="gap"
                     /locus_tag="PKN92_01055"
     CDS             complement(31020..32018)
                     /gene="gap"
                     /locus_tag="PKN92_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNZ47408.1"
                     /translation="MATKVGINGFGRIGRNVFKLLLADPAFEVVAINDLTNAKTLAHL
                     LKYDSVHGVYGKEVSAKDDAIVVDGKEIKVLAVRNPADLPWGELGVELVLESTGIFRS
                     KEQCNMHIQAGAKKVLLSVPPKDAIDAVIVVGVNDDTLKSTDCIVSNASCTTNCLAPV
                     AKVLHENFGIKRGLMTTIHAYTNDQAVLDLPHSDLRRARAAAHSIIPTSTGAARAVGQ
                     VLPALNGKLDGMAMRVPVINGSVVDLVAELEKPATKDEINAAIKAAAEGALKGVLQYT
                     ADPIVSCDIIGNPHSSIFDSLETTVMGGDFIKIVSWYDNEWGYSNRCLDLFKMMAS"
     gene            32369..33685
                     /locus_tag="PKN92_01060"
     CDS             32369..33685
                     /locus_tag="PKN92_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47409.1"
                     /translation="MRTRCLRPLLFFCGIASLAVMVSASLQGCGSCFGSKGTSGDPAS
                     QTPHSPRGARNQSPVSPRASRSQTPQAPGALAVPAVEPAVLPETPELSIQTAGDIKPG
                     MTYEDIHSILGSPGVVIAGTDQDNVVYRWSYAGMSFMGRFENGILVRKTAVQNDDEYR
                     GLQDEDIVSFDKVLYEKIQPGMSYDEVLAIIGMDAQPLTSNSGPVKIYKWTDERGSSI
                     TGRFENSILKRKSGMILEPGKGEGEIEVAPSPPAGAVHEDKEPARISEKESVPTYEEQ
                     EESTAEVSSSDTEGTPAQEKPSRVSVSGSSRRSRTQGSEAGARASERSYHPKVKLPEF
                     KRRLREGVYEIRIHNTTQSNLKVALISEEGGLEMRLAPGRNSSAKVGQGTYQVYFINE
                     NDPFTLHQGQSIPVAALLSDFSVYLFEDTYEVDFLDRNAPRSGNRR"
     gene            33862..34770
                     /locus_tag="PKN92_01065"
     CDS             33862..34770
                     /locus_tag="PKN92_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNZ47410.1"
                     /translation="MKKNFTIAVMIAVAIQIFSVGILAPRLPREVCRPEAATERFFSL
                     EPATAFRLINADGSAQISSSVSAKKIAVKARIRAYTATAEGQVLLEKYLSSLFQIQET
                     PELTTFTTEPIKRPDPIDLRVDYTIEVPQDTDLSLEVSNGNIWVSPGVRNVSITANNA
                     DIQVLEARGDLSIKTINGRIHTEKSCGESTLETINGSIQTSLTSGTLQASTTTGNILT
                     TLLDNEITTCDLTSLNGSITLVMPENPSAEVNAVTLRGIIRADLPMVLAGGPKRRELY
                     GTLGDGYTNVSMNSMNGSIVIQRNTP"
     gene            34767..35369
                     /locus_tag="PKN92_01070"
     CDS             34767..35369
                     /locus_tag="PKN92_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNZ47411.1"
                     /translation="MTREAVLDTGLAVNADSLEEVSDIELVRSCQSGSREAFSELVRR
                     HQHLVYNVAYRYMRDTSSAEDMAQEAFIKAYRLLKGFRGDCSFATWMYRVTSTTCLTE
                     INKKKKRNEIALEARDSDLIADKAPLPEETDMRERIRKCVTRLPNHYATAITLYYLKG
                     VSYDEIAQALEIPLGTLKTWMFRARRQLRKIVEKELRSYE"
     gene            35362..35784
                     /locus_tag="PKN92_01075"
     CDS             35362..35784
                     /locus_tag="PKN92_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47412.1"
                     /translation="MNEHFDCRDDLDLLIAQALAEEPVLQAPPSLLESIEARLDIAEL
                     RAHEERRFRISMTSLLVIFVAALTVAGLSLWFTNLSVLYSEGVSGGKGLFDYYLTAFR
                     MSFSSYQGTYSLAGGLFFSISAVAILAALQIHKMPHSD"
     gene            35836..36885
                     /locus_tag="PKN92_01080"
     CDS             35836..36885
                     /locus_tag="PKN92_01080"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ47413.1"
                     /translation="MHRVTVATLLVMSPLLFAVSTAAPKAPAPLKPPSHASGQMEKDS
                     FSETPSPEKVDSTAPDKALPMPPPPHKSAAWVLEQMSRADNPEFRRRASEAWPLDPDA
                     LETLEPLIRQLSDPSAQVRSAAELRLAEYPVPTLFAYVMRTFTSLDTQKTAWLEVALP
                     SLPAAAGTYFLETLNTEIELPLHRRIAAYVLGRMQYRAALPTLTRHVWSTDMELAGTC
                     VEALRSLHDPQSLDLWIQLLQHDNVYFRPIAVQALAALRSPAALEKIRQLVIHPGNTA
                     AQLSALSAVESYPPEGQLPILIEILEKNPELAPQAHRMLRKQAGMDLGTQAGPWRQWM
                     NTLLTPQPPPLVPSE"
     gene            36949..37641
                     /locus_tag="PKN92_01085"
     CDS             36949..37641
                     /locus_tag="PKN92_01085"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ47414.1"
                     /translation="MRIISVFPALVLATASLSLLSCADDVLRLPTGKATEAPSGEDWI
                     DLFSAENAPLWKNVTDSASTAFTLTENGMKMNPQKETRYIAWTGGSFSDFELHVEFKC
                     GTDANSGVFFRTDPADPVQRGMEIQVFGDHGRHPSVHSSAALYDIATPAFNMALPPGE
                     WNSYDISVDGDQVTVIYNGWKVLDLDLGLMTMPIGKFDTPLASLPKEGHIILQNHGDE
                     VWFRNLKIRPKQ"
     gene            37711..38904
                     /gene="dprA"
                     /locus_tag="PKN92_01090"
     CDS             37711..38904
                     /gene="dprA"
                     /locus_tag="PKN92_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNZ47415.1"
                     /translation="MILTPDQQDWLRLFLVPGIGSVRFLKLLAHFQKPGRVLRASVKE
                     LSDVIGKTVAQRIRQHADAVDMEYHQYQMKTHGVSMVTMDDPAYPVSLAETYDAPVLF
                     FIKGTLLESDQKSIAIVGTRRPSSIGLKTAHQLAYELAARGITVVSGLAEGIDTAAHK
                     GALDAGGRTIAVLGCGLDSIYPASNKTLSEEISRQGCLLSPFVMGTRAIRGNFPRRNH
                     IISGLTMGTLVVEAPLGSGSLLTAKYAAEQGREVFAVPGSAGFSNSQGPNDLIRDGAR
                     LVEKVDDILLELPLAENEFSTFKGLPERPVRQPSPPKMSPAPVAKKEKPSAPSPNSEA
                     PPKALSLSKEEQAVYDVLHQEGSFVDEIALATRLAVPEILVSLTMLEMKGVVRQMSGK
                     RFARK"
     gene            38901..39902
                     /gene="lpxK"
                     /locus_tag="PKN92_01095"
     CDS             38901..39902
                     /gene="lpxK"
                     /locus_tag="PKN92_01095"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNZ47416.1"
                     /translation="MNYIDALAHKIRKDETLPLLLSVLLRGGSVFQRLGMWKRLHCPR
                     VRVAARVISFGNLTAGGAGKTPAVIERALREISLGHQVAVITRGYGAARARKVQILPP
                     GATDPDAVKRFGDEALLIARRVPGVWIVRSANRVKGAQAALEQGCDTLLLDDGYQAVS
                     LERDENILLIDGANPFGNGCIIPRGLLREPLQAMNRATEILVTRCDQTTDEALRKLTE
                     TIRIYNSRAPLSYTVHKPLEIQRLCDGSTLPLSFLQQQSVQALCGIGNPDAFFQTLES
                     LGARREQTTVLADHQALDQVAFSSELPVVMTEKDAVRLGKTDLPNLYALTVSLAPYG"
     gene            complement(39893..40789)
                     /locus_tag="PKN92_01100"
     CDS             complement(39893..40789)
                     /locus_tag="PKN92_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47417.1"
                     /translation="METLQRALEKKLEEKDYFLYRSFKEGLDGKTPDSLAVDLVYENK
                     SRLIFSLLAGSRQGKRTNLYLMMAKNHEEFSAALEQEYAAFQAPGMSGHNGFIPVLRQ
                     GLIYLPDRHQRRDINRELFAWLFRLPGEWAPLYVASSTQWGPRGSEVRRFTVQETEKI
                     KCELIRHLAAAYDPDQFEGVTPRLLSSDDLWVKKNKDRSFSLAIASCRRLDKKLRPGQ
                     FVASLLFGSFGEGQHRLPLAPARPEHFRDALMKALPADHAALLAAFFRQRSLHEKKLS
                     PELKQLLPGADYLDALEACLHP"
     gene            complement(41036..41575)
                     /locus_tag="PKN92_01105"
     CDS             complement(41036..41575)
                     /locus_tag="PKN92_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47418.1"
                     /translation="MSAEKTKKSSVGIFDTVNDLVSHSMKLAGDLIDSGSTASKDISL
                     SSAKHLLKIQQGAMKTGLSLIGKTNKQVDKIIRDRVKEQEWMPEEGKECLDEWADMLD
                     SGLKEFTRVVDSSFEKMLKLVERSGKKKTSGKCKGSAAAPSSCKAESAEAPAKKTTAK
                     KATTKKAPAKKPAAKKKNA"
     gene            41813..42250
                     /locus_tag="PKN92_01110"
     CDS             41813..42250
                     /locus_tag="PKN92_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47419.1"
                     /translation="MYCKQSAAITVLMLIAVLLVSGCATTAGGKSDEEMVKETASLWA
                     QALVEHNVEKLLSTISESFAAPQMESADKAMLGTFIQQAIDSGYLDDAEVSFEDAEYE
                     LKDGEYFIYPIDLMGSAGSVSVELGLKKENGTWLVSSMSVDGI"
     gene            42337..43734
                     /locus_tag="PKN92_01115"
     CDS             42337..43734
                     /locus_tag="PKN92_01115"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNZ47420.1"
                     /translation="MSASLDEVLQAIRTGIPLFLERFQECLRIPSISTAPENQPDMLR
                     MSQWLQAEMRRIGLEHVQAYPTPGQPMVYGDWIHAPGRPTVLVYGHYDVQPADPLDEW
                     VSPPFGAEIRDDYIFARGASDMKGQFLAQLAAVEAWLAQGTCPVNLKYLIEGEEEIGS
                     PYLDDFIRDHTDLLECDIVLNCDAMIHSAENPSITYALRGLAYFELEICTAEKDMHSG
                     MFGGAVRNPIHVLTEVLSGMHDASGRITLPGFYDHVPTAEDDERALLAKAPYNDATFL
                     KTAGAFALYGEEGFTTLERLGVRPSLDVNGIWGGFQGEGAKTVLPARAGAKLSMRLIP
                     GQPLDMVEKQLRAYLEAKVPAGCQWNLTCHSIGPGSVMHWKSPYMQAAADALKKVFGK
                     EPFLRREGASVPVVRLLQERLGVDSVMLGFGQPDDGIHGPNEKQSLPVLYKGMESYAC
                     FLYNVGRLKPMSVHD"
     gene            43727..44482
                     /locus_tag="PKN92_01120"
     CDS             43727..44482
                     /locus_tag="PKN92_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNZ47421.1"
                     /translation="MTKERLDILVQERFQLTRSKAQGLIQTNCVRNSAGEILNKAGMR
                     LDPDTVLFLSEGPRFVSRGGEKLTRALEVFPVSLQNRVVMDVGASTGGFTDCLLQHGA
                     AHVYAVDVGYGQLAWSLRQDSRVTVLERCNIRTLRKEQIPLSPTFFTVDCSFISLKVV
                     LPCIVDLLSPVVEGIVLIKPQFEAGRHQVGKGGVVRDPAVHTEVVEGISRFAAGLGFS
                     EVDSTPSPLLGPAGNKEFLAWLRGFTPQESIGT"
     gene            complement(44483..44971)
                     /locus_tag="PKN92_01125"
     CDS             complement(44483..44971)
                     /locus_tag="PKN92_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47422.1"
                     /translation="MPLSFDEMRDIYNRTRIIRRPTYGIVKGYHELPYICLGAGLDGT
                     RGSLYVRGRIHVSPQFLIKPRHYQSKYEDIFGSEEVDVEIAGRVFGFLGFPNRPVECS
                     MDKFEVKHLSETVDDLLSKAIDELERKEDITTGVIITPESQYYQISIERFIAYIIEDE
                     FR"
     gene            complement(45045..45593)
                     /locus_tag="PKN92_01130"
     CDS             complement(45045..45593)
                     /locus_tag="PKN92_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNZ47423.1"
                     /translation="MNIPVKKSFLCACVLVMCLAVSGCSVSIHDAIAHESTEAVLQLL
                     KEHPEYLTARDGKGKTPLHTAVTYKKIDLIPLLVERGAALDATDVTGMTPLHVAAMLG
                     RSEEAKVLMSLGASPELTDDYGDMPLHTAAVFGSGGIIQLLVRTGMAPDTPNAKGQTG
                     LEIAREYRQERVVALFEHLVRR"
     gene            45842..46948
                     /locus_tag="PKN92_01135"
     CDS             45842..46948
                     /locus_tag="PKN92_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNZ47424.1"
                     /translation="MKLGIEITPLTQQPTGIGYYTLHLLSALSALPDCPEICGFSSGL
                     HKPQTADLPISFRRIAVPTRALYRCWRLFNAPKADAFLGGTDIYHAVNYVLPPLKKAA
                     GVLTIHDLAFLAHPEWCSPKIVAPFRDAVTRHARRADRILVPSEATRKDVLRFLDVAP
                     DKIRVTPLAGDPSFFPMPREEAEEQLREHLQLTGGFLLCVGTLEPRKNLPTLLRAFLK
                     TDLPHKLVFVGGHGWGDALQAQVPLSAQDKKRIVFTGYIRDRGLFPALYSAATAFLFP
                     SWYEGFGIPAVEAMACGCPVIASDTSSLPEVAGSAALYAAPGDTDAWARAMEHLCADI
                     TLQEDLRQKSLAQAEAFSWEKCALATMRCYKELL"
     gene            46948..48051
                     /locus_tag="PKN92_01140"
     CDS             46948..48051
                     /locus_tag="PKN92_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNZ47425.1"
                     /translation="MRILLNAMQSENRSGTGRYVTELARALHALAPKEELQYLCPDGK
                     IRCFEEAFLQKPVTPLPLCKRVLTEQWKLPGLVRKKHFDLLHYPAGTGLLFASAPHVL
                     TIHDLCYLKNPDWFPKSRARYYSFVMGKNVRHASRIFADSQATARDAEALLSVPSEKI
                     DVIPLGISPDFSPAGPEARKQLRQCLLLPERFFLFVGTLEPRKNLTTLVRAWARLPEY
                     LPELVLAGRPGWKITQDALRNASGAQAHRLHFLNYVPAELLPALYSEATVFVYPSLME
                     GFGLPPLEAMACGTPVVTSNTSSLPEVTGDAAVLISPEDETELAGALEWVAGDETLCS
                     RMREKGLSQSARFTWEKTAQLTLAGYRKILGGY"
     gene            complement(48072..48719)
                     /locus_tag="PKN92_01145"
     CDS             complement(48072..48719)
                     /locus_tag="PKN92_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ47426.1"
                     /translation="MDNDFTEIRETNEAETRLLESALQLFSEKGYEGTSIREIIEGAG
                     VTRPVLYYYFTNKEDLFRKLLEPALAKYTRTLADIRENYSDAVGRLKAIARATFRFAE
                     ENPQAVRLVLQLYFAPPKGGPRLDKTIYRLRRFRFLEEIMQEGLEKGELAGGDAQGLA
                     LALIGMMDTFVTAKSYLPDTHLSEEAAEGLIDLFYFGACYKENPPSILTSPFKYI"
     gene            48902..50320
                     /locus_tag="PKN92_01150"
     CDS             48902..50320
                     /locus_tag="PKN92_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNZ47427.1"
                     /translation="MTQFFCGIDFGTTNSSAAVSTAETVHLLDLDPANDAPVSLPSLL
                     YISREGEKIVGRQAADLFIARNIDREIETAEVSLESLIPAFTRAEPDKDESYNPLFTD
                     PNIVEAVNAKITVEVGSPGRLFQSLKSLLRQSSFLYTEVFGIKYQMEALISYILKVMK
                     EAADTHAGYPIESAVFGRPVRFSEKENEDQLAERRLRTAASLAGFKNVVFFYEPVAAC
                     VEYAADTRDSQKIMVVDIGGGTCDVSIMEFAAAEHLADRLSSSRVLSVSGTPVAGDEL
                     DKAIIRSRLFPLLGSRAHYGPSKLPMPQYIYSQVLDWQNLYKLNTESQINWLLAAEVS
                     SNYPEGLKALRMLIENNYGYLLSKEAEAAKKRLSTESTTSITVDAAHLHLKDTLDRSE
                     FIEIIEDTLDKMFGCILEAEEKAGLRPDDLDYVLTTGGTCLIPAVYALLEKRYGAEKL
                     KRRHSFTSVAAGLATVARYVFR"
     gene            complement(50329..51702)
                     /locus_tag="PKN92_01155"
     CDS             complement(50329..51702)
                     /locus_tag="PKN92_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47428.1"
                     /translation="MTNSFSDKHLSADQAARWVSAIPQPFTLIHNPPYVFPPWELCPM
                     AHVATELADGAEGVVMDMDGTTTTTEVLCIESLAKMTAAMCGRSLENASQYLDPVRDY
                     PNIIGNSTTKHVEYLIQQYGASFQVESICRHFLESTAWTISQGMDVNRKKEALNSLIV
                     LGLSELDQDPDWNRLLSAGEPERQEGLTALVSRCLGRLTVNSLTEQSRAGIEIYYRHY
                     HATLAEIRKQGGSSGGAGKAMIEPMPGIGITLALLKGWLGEELAQLRDPLIAALLERN
                     DSADTKTVLSREGLAQLGRYFERHPVKLALVTSSIGSEAAIVLDEVFRVLVSEAGDWK
                     ISSGRKDVLLDAFQSPARVYDAQITASDSSEIRLKPHRDLYSMALHTMGISPEDFHKV
                     AGFEDSESGTIAIRTAGIPLCCALPFAMTQHHRFEAASMVCIGGLPEVILNRNFFLPE
                     HLTAGSD"
     gene            51946..54093
                     /locus_tag="PKN92_01160"
     CDS             51946..54093
                     /locus_tag="PKN92_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNZ47429.1"
                     /translation="MKQVAVTGGKISVEEVPPPVPSEGMALVQTAFSLISSGTESAFL
                     STGGAAGLALKKVLDPVTREKFKHKLASTGIRSTWTLVQNKLFDTQAPGYGCAGIIAE
                     TGPGVLHLRQGDRVACSGVGYACHAELNSVPHQLITPVPDGVDLDEAAFVSLGAVACQ
                     AVRRAAPSFGEIFIVSGLGLVGQLTVQVLKAAGCQVIGCDPLPFRRQLAESLGADLSC
                     APEDLAEASWQNSGGFGVDGVLVCAASKDSSAVNEALRACRQKGRVIVAGAVGMHLER
                     EALYLKELDFGLSCSYGPGRYNPLYEEQGLDYPIGHVRWTQGRNMAAFLLMIRDKRVQ
                     VRPLIHLQVSVDEAPAAYDAIQHRKEVIAALLEYPAPAQQSAQTPFKRVYVLRASPNK
                     KKELAVSVFGAGAFASGILLPALKGVPGCRLHSLACATGLHAQKWAKRFQADACSTDY
                     KELLKDPALDAVFITTRHHLHKAMALDAIAAGKHVFVEKPLALTTEACEEICDAAQKQ
                     GVLLAVGFNRRFAPLSLRAQQLFSGTKQAHLLLYRCNAGALPPDHWALDPLQGGGRIL
                     GEAVHFFDYACWFFQDVPRTIQAECAAPAQRGKGIDSVSVSLLFSRGALATVLYTGDG
                     SSLLEKEYIEIHGTESSLTIHDFKQLTPYGIGAGKITARRIDKGHTSLITHFIRAIQG
                     HEALSVSGEDGRLATYIAEETLRQINAFHKDTL"
     gene            54093..>55210
                     /locus_tag="PKN92_01165"
     CDS             54093..>55210
                     /locus_tag="PKN92_01165"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNZ47430.1"
                     /translation="MRSSLLHFLLSLSFSFLLCLAVPVFAEVKPSDDLSELVDNISIS
                     QGWGNPGINTAAWMAEGSQQPLRIGDTVFEKGIGTHAQGAIRIPLNKGYNTFSAKTGV
                     QWQGGGKGSVVFKVQVDGKSVYTGKSMSDSDTPAALHLSVQGADELLLLSHDNGDGIS
                     CDMANWVDAQLIRDTRVPVIGVPVWKFGSKDASPLAEMEGSFALAGQSGNAQAAFCVP
                     LRCATLFTSPGQDIAVSFPLAGISESFTIEAQLQGVGTAKGIATLSLASGETVRQPLT
                     EDVTTLTVRGNSGTAEESLTLTIQTNCSALVRLKSLTYSCDGIKTPLPFFPPQRLAAG
                     SDAPPAIAMQPLLEQEIIEWDWRMQDGIGAGATRNSYA"
BASE COUNT        12806 a        14917 c        14027 g        13450 t
ORIGIN      
        1 gggatacccg ccaggcggtg gtactacacc ggcaggaagc ctggtcacga gatcaggacg
       61 ttttgccgga gcactaccgt aggcacagat gaacaccatg ccgagcgata aaagcagcag
      121 cagaacaatg cctgtccact tgatcaggga gcgcagatat ttcacggtgc atcctctccc
      181 gaaagccggt tattccggta tgttttattt tgtgcctttg tcctcaggga atctgcatga
      241 cctgctcgcc tgtttccagg cggtgccagg tacggaactt gcgttcccct tctatcaatt
      301 caataacacg cccgcctctg ggaaaccctg ctttgccgta cgttccgtgt ccgccgcctc
      361 tgccgtagcc aaggtaaatg ccgtccactt caccgatgaa atcattggta tggtcatgac
      421 cgcaaaaaat accgacaacg tcacccgttt ctttgaaaac atcaaagagt ccggaattca
      481 cgggcgagtc gcaaatgtct tcctgtttca cgccgatggc gtttccggac tcccaggctt
      541 tgcggaattc gggaagcgga atatgaaaga atgccagaga aggaagcgct gcacctccgg
      601 cagcttcacg acgccgggtt gattcctgct tgaaccattc cagctgggat tcttttatgt
      661 gagcataacg cgattgccct tcccagatca tgtaggacag agaatcaatg aggaagatat
      721 tagctgcagg cttctcggga ttttttgagg aaccgataac cagactgtaa ttgctcaccc
      781 cgtcaatgtc ttccggtccg gcgacagaca gggaatacgg aagcccgctc atgatggaca
      841 tcagccgttc acggcttgcg ttgccttcat cgtcatgatt gccgaacaca gccgcccagg
      901 ggatttttct ttcagcacag aacgatgtta cctggcgcag cgaacggttc ggaagcaggg
      961 tgccgcctgt aatattgtct ccggaaaaaa caaccagatc cgggtcttcc tcattcagaa
     1021 cggtgcgtat gagctttgca gtcaatttgt cttcgccgta cccgtacgtc caatgaagat
     1081 ctgcgaactg tacgattctg aaagtgccgt tatcacgaaa cttcagcggg attttctgtt
     1141 cattttctgc aaaggcaccc aaactcaggc tgagcactgc tgtcatcaag actactttat
     1201 tcattcacgg actccttctg tcacaaagcg gaagccgcac gtttcttgca caactcaagt
     1261 aaaacagata cggcttccgg tatttcagac gctgttcttc atttgacggc gttgaaaatc
     1321 aggcctcggg tggctcgttt tcagcaccga ttacttttgt gcgttcaatg gactgcaatg
     1381 ccagatactg atcgccgaag cgtttcagat gacccagttc cgtgtcgaaa aggtcgaaat
     1441 gccgttcctc gtcttccacg agtttttcga acagtttttt ggacactgaa tctgcatgac
     1501 tgccacattc aatagcaaaa ttattgtaat cgcgcaccga ctgttcttcc agatgggacg
     1561 ccagtttcag catttctttc acgtcagcaa tcttctgcac ttccgctgat gctgccagtt
     1621 cgacttcgcc gcccagaaaa aagatacgct ctgcgagggt ttcgatatgc agcatttctt
     1681 caatggcaat gcggcggaaa agattggcaa gcatgtccag gctctggtca tcacaccgga
     1741 aatggaaata catgtactgg tgaaccgctg tcagttcgtc ggcaatggct ctgttcagca
     1801 aatcaatact cttttcgtgc atggtagatc tccttttgat tttgtttata cacgcatggc
     1861 ccgattcatt tttttgatta tataggttcc ttctctatct atcaaaactt tttccggtgt
     1921 cggcaacagc aatttctgca aaaaaaagat caagtcgctt atctcttttc ttgaatcaac
     1981 agctccggtc gatattcaaa atgcatggtg tcatagtggt gccatttccc gccccagatg
     2041 aagccctcac tttcgaagat ctctacaata cagcgaagcc ggtcatccga taagatgcct
     2101 tccggataaa ccgaaccttc ctgtgtccct ttctgatccc agagccagta gcgataaaga
     2161 cggggcggca gttgaaaatc tacagctata ccgaaagagt gcatgcttaa gcgtcgggtt
     2221 ccggcgattt cgcgccagtt gaaactgccc gacggattca ttatatagcg ctgtgccgca
     2281 gggggcagct gcatcatctt ttcccttacc cgcgccagtg cctcatccgc tccattgacg
     2341 cgggtaaaag aaatgactgt tccgttaagc caggggacat ctgtcagctg cgcccgcacg
     2401 gcatctgccg actctccata tatttttctg aaaaaggact cattccgtat ccgtcctgga
     2461 tcttcattaa cgccgggcgc cccgtcacgc caatgtctcg gatagggctg cgccatctga
     2521 tctttcaggg aagcctgatc gagcagcacc tcaaaacttt ctttttctat tccgtcgtca
     2581 taatacagaa cggtgccgtc gtcccagcaa agcgctttct cttcctcaca aatccggcag
     2641 tacggcgcat aggcagccgc cagcgcataa agtcccaagg gctcgggttg cggttccgct
     2701 gtattgcctg ccacatcggc agcaagacat agcataaagg acatcagcag aaacactgcc
     2761 gttgttgtca aaaaagatct catgaagcaa tcagctcttt tgtccgatgc gtgacaagat
     2821 ccattcataa ttccgccgtg ccgacagcag gggattccgg tcttcgggtt catcttccca
     2881 ggaataccac ccgtcatagc ctatttcagc gagagccgcc atacaggctt ccacctgagc
     2941 cgttccatcg cccaacagac aggtcaaatg tgcgcccggt gcttccacat ctttaatgtg
     3001 ggtgtggcgc acgaaagcac cacattcccg tatgatctgc gggccggcgg ctccctgcgt
     3061 accgagccat cccgtatcaa tgcacacacc cagccattca ttgccgccgc caacggcttt
     3121 caggatttcg tccgcggttt tttccggatg attttccgta ttgaaacgga tgcccgatct
     3181 ttcgcaaagt gccgtagctt cctcgggtgt attctttccg atccctccgt taatctgcgg
     3241 gatatccagc cagtggcaaa tttccactgt ttccgacgat aaatgaccgc catacccgat
     3301 ggctttcaat ccatgatcat caagaatagc tttccagacc cgccctttct ctctgtccat
     3361 ggattcaggg gctgcatgcg cttcccatac ttccactgca gtaaaaccca gaccggcaat
     3421 ttccttacag atcttttccc aggcagcggc atctgtggcg gcaacagttt tgttgtgctg
     3481 atcgccccag ttctccagtt taaaccgaaa accggtcact tgcgccacca gattagcagt
     3541 gacgaaagaa attttcggtg cgactttttc ttcttccgcc tgcacgaagc gcgcaacagc
     3601 tggcatcagt cccaatgcgg tacctgcagc aatggtttgt cctatgaatt cccgtcgact
     3661 tctcttctgc tgtgtgtcca tgcatatttc cttttttgtc gctgttcact ggcaagatta
     3721 aaggaaccgt tcttttcatg taagataata ccgcttcggg catcctgccc gccattttat
     3781 ttcaaagttc ttttcggaac aaacaacatg cttttaaaat accaattttc ttttatcgcc
     3841 gtgcttctgc tgctatcatc aggcacttat gcagaaccat atagaattgc tctctttcat
     3901 gaacccggca tgcctgcgcc tgccattact ccggaaccgg atttctggga aaacatggtc
     3961 gccgggctgc ccggatttca gatggaacgt agaggcttaa aagacctgaa taacccgacg
     4021 cacttttcaa gggaccactt cgatcttctg ctgattccaa caggcgcctc ttttccggct
     4081 gcggcagcgg atgcgctcat ttcctttctg cacaaaggtg gtgacctctt gacctcaggc
     4141 ggctatgctt ttgatgcgct ggtgattgaa gatcagggtg cctggaaaac acagacagat
     4201 tttctggaga atgcggcgtc catcgccaga aatccggaac attccgctgt gccgggcggt
     4261 gatttcgaat cgccggtctc ctgctggatc agtcataacc cggcatatgc aggcatcact
     4321 accgaagtat cttacaccgg agcagcctgt gcttatgcac tcagcagtct cgacggcgga
     4381 accggccgct gggaatgcgt tctgggctgt caaccgggca accattatct catcggagcc
     4441 catttgaaaa cggatgcgct tctgggcaga ggctatgcct atcttgcagt ttaccaatac
     4501 gaccgggaag gagccatcct cgattttact gatttcgcac aggtcggcaa ggcgtcggac
     4561 tggatgcggc atgaagccgc agtgacaatt catccgcaaa cagacagaac cgtatttcag
     4621 gcggggctgc atcatattca gggtaccatg tggtttgatt ccgttacctg tgcggcaatt
     4681 cccgaagaaa tccgcatcaa tgcacactac ggcaaaccgg gcgacggact cgttgtcaga
     4741 aaagaacagt tacaaatctt cagtcctgat cgccgcttta cgtcttctgc cgatgcctat
     4801 gctttttatg cacaaacaga gtcaggcaga aactccgagc ctccccccgc actccatggt
     4861 ttcgaagcca cggcgcagtt gtcgggtaat gggcatatca gtcctctggc gaccttcgca
     4921 actccggaag aataccttcc ctgcccgggc acacttgttt tcaatcatca gggctttttc
     4981 aaagactcgc attgggcgct ttttggcgtg gacaatctga attttcctgc gctggcacct
     5041 gatgcctaca gagaagcgct tctgcgcctc cgtgccggcg tcacgatctc cgagctacgc
     5101 cctgcttatg tctcctatca gccgggagag tcaggatccc tcagtgcatc agtccgcaac
     5161 acatcaaagg agaagcgtgt tctgagagtt tctctggcag ttgatgacct gttctgcgcg
     5221 gcactgacgg atcgtcctga acaggaggtc gctctcgcac cgggagaggc tgtaacgacc
     5281 agctgggcat ttaccctgcc ggaaagcgat tcccttcccg ctttgtgcag cattgtcctt
     5341 cgtgtctatg aaggaactct tttgtgcgac gaaagaacaa caggttttgt ggtaaaccgc
     5401 ctgaaccgcc tccctgctgc ctgccgtaaa gcgccggagt cctgtcgttt cggcaccgac
     5461 ctctggtctt ctgttttcct ttcgccctct cattcgccgg tcagttggag cgaggacctg
     5521 aaactgatga agcagtccgg gttaacggtt gctgaaatgc ttcaactttg tccggcagat
     5581 tatgagtata ccgaagagca ctggcgcttt ttcgacggat ttatccaact ctgtgcggat
     5641 cagaggctga cctatatggc aggactgctc attggcaaga atgtgtgcat cgacaacgat
     5701 actttaaaaa agcaacaggc tctttgtcgc agttttgccg aaagatacaa gaatgtacct
     5761 gatctgattt attatctgaa cggtgatttc aggctggatc tggcggatac ggaagatata
     5821 cgtgcccttt ggaaccagtt tctggcgaca cgttgtgccg atgctgcaag tctgaatgag
     5881 gcgtggaaaa gcgaaggatt cccggctcct ggcgctgtgc ctgtacagga gtatgcggcg
     5941 cagggctggt atgacctgca tgccagagat ctgaacgaat ttaaagccct tctgatgaat
     6001 cgctggattc aggcgctcat cgttgccatc cgggaagagg atccgcacca ccccatcacc
     6061 agtgaatact accagcatcc tttttccggt attgatcttc gtaacagtct gggcgggcag
     6121 tatgcttcca atttcggata ttttgaccgt cctaatctcg acagggcacg gtttgccgct
     6181 accgcacgca tgtatgacat gcgccgggaa ggcaaagcac tgcatgtggg tgagttcggt
     6241 gtaaaaaccc atgatgcctg ggctgaggaa cggggcggta ctcattacca catccgacgg
     6301 agcgaagagg aacagcttcg ttttttctgg gatattgtgc atcatgcctg ggcactggga
     6361 ttcagccaga tacaaaactg gtgctggcgg gatgatccgg atcgcatctt tccctggggc
     6421 gttgtatggg caaatccgtc acgaccgaag ccggttcttt ccctctggtc gcgtctggcg
     6481 ggtatgaacc gaatgattac ggtgcagcat gaaccggccg atatcgtatt tgtattgtcg
     6541 gacggctggc gcagcggggc gcctgaaagc ttttcgctgg aagttatgtt gaatgcactg
     6601 gactgccttc ttgctgcaaa tatacccttt gacgttgtca gcgaaaacgc cctcaccacc
     6661 cttcaggaca atctgccgaa ggctattatt tttccggcag cctatgcgct ttcagatgaa
     6721 acactgtctc tgatgcaagg ctatgcgata cggggagcgg cagttcttct ttccggtgat
     6781 cccggcataa atccgcaggg acaaagaaat caggaacggt acaggcaatt tctgggctcc
     6841 acgtttctgg cagaaacagg agacgcaaag ggactacctg ttccccggat aaagacggag
     6901 acggcgactt tatgcctgac tgaaggcact gttacgcttt atgcaattcc gtgcggcgcc
     6961 ggggcaattt actggtcacc ccttctttgg gaaagtgccg tctccgccga tctcttcgga
     7021 aaagaccggc acctgactgc tgatccccgt caaaacctct atctcccctt tctgaaaatg
     7081 cttggcttct ccagccctct gcctgtctcc aacacctctg gaatctggcg gacatccctt
     7141 gttccttccg gggaaaacag gctgatcagt ctttttcctg ttacagggga aaatacgccg
     7201 ggaacagtca gcgttgctta cgatggcctg cagtttcaat ttgaatatac atatgcggcg
     7261 ccctccgctc ttctgctgaa cccggagaat aaaattctgg cgttgtccgg atgcggctcc
     7321 ctttctgtaa acggcatacc agtggttcaa agcaaaggaa tgtggcttat ctattcggat
     7381 gaaatgcttc ctttggaggc gtgcagccgg gttcatgctt tttcagacag ggaagccgtc
     7441 tttttcccgg gagacaaccg ttccctgaac tgcatgcaat ttgagcagta ggcggcaggc
     7501 aggcataata gacagaaacc agcacgcttg tgcagaatcc ttttactgac ccctttacct
     7561 cgagaggaaa tcgcaatgca gtcgtcacgc agacattttc ttgccggaag ccttcttgcc
     7621 ggtgctgtcg ttctggcaaa atcttctccc cttaccgcag cagctgcatc tgcagatgcg
     7681 ccgtggaaat gcggtgtcgg gctgaacgga ttcatgtcgt caggcgacca ttttaaaaag
     7741 acctatccgc tttgggaaat actggattat gctgcacagg cgggctttaa tggtgtcgag
     7801 ttggttgaag gctggcccat gggcggttat ccggattatg aggaagagaa acgggtcgcc
     7861 gcattgcgcc ggctctataa ccagtacggc ctgcagatct atacgatgca gacaggcggg
     7921 gcagactcct atgcttccag tgcagaagca cgccgggaat ggctggcacg tttccggagg
     7981 ctggctactc tttgcaaagc cctgggaggt gacttcatcg gccattggcc gggcggcggt
     8041 cttgagggaa ataagactgt ggatgaggcg attaaacatt tgggcagcag ttaccgggaa
     8101 gcatcaaaga tctgtgcaga tctgggcatg catatgtctt ttgagattga accgcctttc
     8161 cttttcaata cctatgagca tctgatcgga attctggagg cggcggatca tcctgcctgc
     8221 agaaccaact atgatcccag ccactttgat gtcatgtccg gaagcaaggg aaagcctgag
     8281 gatatgctga aacggcttgg cgtgcagcat atcgggcatg tccatctgac ggacacggac
     8341 ggcacccagt ttaacggcac atcaacccat ctgccctgcg gtgaaggaca ctgcgacatt
     8401 caggcttctc ttcatctgct gcatgaaggc gggtataaag gttggatcat gattgatgcc
     8461 tggatgactg aagatgccta ctacaccacc cagaaaggca ttgacgccgt ccgggtggca
     8521 cagcaggcat tttctgcaga ataaatcgcg gcgcccttat tcagaaagga aggacttcag
     8581 aacaggctca aatgcatccg ccatccgctg gaatccaaga tcggtgggat gcacaccgtc
     8641 taccgtcgcc tcgccgtccg atccgagaag ggtgtcgccg ggaaggtaat aaagatcgct
     8701 gatatactcg ctgcccagca gcgtaccgta actttcctga agtgcctcgt tcttttcttc
     8761 ataagcaatg cgggacgcct ccagaaaagc gccgctctgg tactggatat tttcaaccag
     8821 cagaatggga atgcccggcc ttgcggcacg caatgccttt accaacggaa caacccgctc
     8881 cgcaatcagc tccggcgtca tgttcgggat acaatccaga atataagcgg cagcgtcaat
     8941 ttcctgcagc agctcgccca tggcggcttc cattttccca ttgccggaaa aaccgaggtt
     9001 aaccacaggt ctgtcaaaac gtctgccgag aatagccggg tacgccatgc cgggccggga
     9061 ggcacaaccg ccatgggtaa tggaggtgcc gtaaaacacc agcggtttct gtttcccttc
     9121 aggtcgcgcg ggtgctttgg caagagaggc ttctttgggc acaccgatcg caagcgaggc
     9181 ggtgccgtta taaagaggca gatacagaag atattcatga ctgcccgcag gaatcccgga
     9241 agcgagcact gcctgcgttt ctttttgaga aggacgcccg tttccgatcc agtgccatcc
     9301 tttatccaga cgcacataca gatccagacc gctcacaccg gtggcaggca tatggggcat
     9361 ggcaagattg tcgttggtca cttcccattt ggctgaaatc ttctccgcat cgctcacaaa
     9421 atgcaccgcc attccggcag aatgtttgct taacttccat acagaatcgg ttacaacccc
     9481 ttccgctttt gtaggaagac ggtcgtaagg tttttctgtc tctccccaga ccttcccttc
     9541 aatgcccagg agcagtgcat cgtaccagat ccattctccg tcgggatcgg ccactgccat
     9601 attggggtca agtttttcga tggactctgc aaaaataacc ggaggggaaa tcagcagagc
     9661 gattgcaaaa agaatcagca gcgactgtac cgctgtatat ctgttcatga ggctcatttt
     9721 ccttgtcatt aacttttaca gcccttttac atgccatccg gtgcgataac gccgcttgac
     9781 gtaccggttg gcttttttat tgtttgtgaa tttcagtgca cctgcatccc actccagttt
     9841 ttcgccgata aagcgattgg caatgttcgc cagaaggact gcttcggtca tgggtccgga
     9901 aaaaccgaaa tgggcacctg ccgtccggtt gcccagagcc gcctccacaa aatcatggta
     9961 gtggttcatc ggttccagtt cgggcgcggg ataatttgcg aatttttctt cagggaaaag
    10021 tttcggcggc cctacatgcg gaataaccag actgccttct tcaccaatca gcagagaacc
    10081 gctcttcgga agctgatacg cttccgacaa atggggagag actgattttt caggcatctt
    10141 tccaccatcc atccagaccg cttctatcgt tttccccacg gtcagatcgg agccgttgaa
    10201 aagatagttg gcgatcgtcc aggccggata cacttcttcg gaatagcatt ccgcatcaca
    10261 ttcaatagtg agcggtgcac caatgttgag ggccgtgaat acaggatcaa aaatatggca
    10321 gccgaaatca ccggtggcac cgccgccgaa atcgcgccag cagcgccagt agaaaggatg
    10381 gtatacctca cggacataag ggcgcatggg cgcaacgcca acccagagat cccaatccac
    10441 atgggctggc acgggatctc tgtgggcggg ccgtttctta tcgtccgtcg tatacacggc
    10501 agcggaccag gaataccagt ttttcacttt accgatggca ccttctttca cccagtgaac
    10561 agcagtgcgg taatagtcgt gagaatgaat ctgaatgccc atctgagtgg caacaccgta
    10621 ttccttggcc gcttccgtaa ggcgccgggc ttcataaacc tcatgagtca gaggcttttc
    10681 acagaagaca tgcagcttct tgcggatggc agccatagcg ctcggagcat gaatatgatc
    10741 cggggtggca atattgatgg aatcaagata ctccccttct tttgccagca tttcgcgcca
    10801 gtcgcgataa agctttgcac ccggatagcg gacggcagct ttttccaggt tgttcgcatc
    10861 aatatcacat aatgcccgga catgtacttt gccgcttgaa aaaatctgtt ccagatcaga
    10921 agtgccctgc cccgtcacac cgatggcggc atgattgagt acttgcgtgg aagcggaggc
    10981 aggcgttttt ctgctcagga ttaacggaac tgccaccgca caagcggcac tgcgggtgat
    11041 aaaagaccga cgtgatattt tcctgctcat taaaaactct ttccatacgc ccgtaggcat
    11101 tctgacgtaa caggggcgac cctgccccgt ggtctatctc taaaagactt cacaatagtt
    11161 aaaaaagggt ggctaattca agacaacccc gaaaaggggg cgattcgttt gtctcccacc
    11221 tggagatttc atggaggggg caaaaaagaa aaacatttca tttttcgggg ttttgaatga
    11281 ctcccggtct tgtgcaataa taccgctttg ggcgctttgc cctgtttttt ctgttttgtg
    11341 tttcttcgtt caggaagtgg aaggtattat ggaaaacatc atcggactga gcaatataga
    11401 cgtttccatt atttttgtct atatgctcgg cacgctcctc ctgggtctct tctgcacacg
    11461 ctatatcggt aatgcggaag actttttcat ctccggcaag gcattgccct tctgggcaat
    11521 cggcttctcc atcgttgtca gtgatatcgg tgccacagac tttgtcgctg ttgccggtgc
    11581 aactttccgg catggtgtat cggccgccaa cttcgactgg atgggctcca tgcccgccat
    11641 ggtctttgcc gcctttgtct ttgtgcctta tttctggcgt accggtgtct tcacgatccc
    11701 cgagtttctg ggacgccgct acaatgccgg agtacagttc ctgaacgctg ctatctgggc
    11761 ggtggtgctt ttccttatgc tcgccatcat gctgtgggtt acagcagaca agctaatgca
    11821 taccgttctt gggtggaacg ccaagtggtc ggtatggctg atggcaatcg tcaccggtct
    11881 gtacacgttt tccggcggac ttacggcagt cgtgtttacc gacgtagttc agctggtcgt
    11941 tatgtatgtt ggcggtttcg gacttctcgc cctgtcactt tgggaagtcg gcggctggag
    12001 tgcactgcgt gaacaggtac tggcaaaggg cccggaattc cagcagcatt tcacccttct
    12061 gcttgagcac gatacgacag gtcccttccc ctggactggc ataatcttcg gtctgggaat
    12121 tgttcttgcc atagcataca tgagcggaaa tcaggtcatt gttcagcgta ccctcggtgc
    12181 ccggacagag tgggatgcga aaggcggtat gcttttgggc ggatttctga aatccatgat
    12241 tccgctgatg gtggcacttc ccggtctgtg tgcattgatt ctggttccgc accttcccgt
    12301 tgaagaagcc gaccgcgccg tacctgaaat gatccgtctg ctccttccga caggactgcg
    12361 cggactcatg tttgccgccc tttttgcagc gcttatgtcg agcatctccg gaacattgaa
    12421 ctcggcaagc acgatcttta tcaccgatat ctggggtcag gggctgcgac ttttcaagtg
    12481 caagccgctg agcgagaaag gcgcgcttaa catggggcgt ggctttaccg cttttctgat
    12541 catcacgacg gcgattctgg caaagcccat tgccgaccgg gaaagcattt acgttttcac
    12601 ccaaacaatt ctgagcatgt ttcagggacc gacccttgcg attcttctgc ttggcatcac
    12661 ctggccccgg gcaacccagt ggggcggtct tgccgggctg gcattgggcg taatcttctg
    12721 ctttattctt aattacaccc ccgggctttt cacttcgagt gaacctttcc tctttgttgc
    12781 ctggtggtca tttgtctttt cccttgtcgt aacggttgtg gtcagcctgc ttaccccggc
    12841 tgaaccggaa gaaaaactca gaggtcttac ctggggttct gtggttcaca gctccgaagc
    12901 gcaagccgcc cttgaggaaa ggaattcctg atgcatttag aaacgctgct gcgaaaatac
    12961 tgcgccatta ttttttatcc gatcttaagc aaaattttcc accccatcaa ccatctgcta
    13021 gcacctattt acaaaccctg ggcaactatc gccgccatgt cattctttgt gggtaccatg
    13081 atctgggtcg gtgttattct caaggaatct tatgtgaatg aagggcggcc caataaatcc
    13141 atctggacag atctccgcct gtggactgtc gtttccatgc tcccccacgt ttttgtttac
    13201 tggtatttca gatagggaat tgatatgaac aacacaccgg acactattga atctgatgcg
    13261 aaagtaagca aagacacctg ttgtagctgc gaagggacag cgcctgccga tgatcctaaa
    13321 tgtatgaaaa agcgggacgg ggaaaccaat attgcagtgg ggcttttcct ttttgcgctg
    13381 ggaattcctg ttctgctggg cacattgggg attgaaggga atgtgcgtgc cgcaactgtt
    13441 aatgctgtgt gcggactaac cctgctgagt atcggcggag gctgggtcgc gtacggctgg
    13501 cgccagttca agcaagccac acgcagataa tctgttgtga ccggtctttt ccttccgctg
    13561 cgtatccccc gggatgaagc gcgccgtctg ttttatcgca ccgggatcag gaatctctat
    13621 ggtctcatcc gcaggcgtcc cgccaaaatg ggtacagacg gactgcctgc ttccgtagaa
    13681 ttgctctggc ttcctgccta tgccgtccgc tttgctctct ctctcgggga aatacaaagc
    13741 cagggctggg tttctgtaga tgcaaacttc gggggctttg cgctgtttga gcgtgttgac
    13801 attctggaag aacgtgaaga aaaaggcgaa tgccttccag taatcctctc tccggatgaa
    13861 gcggataaaa aaggaaggca tggtctcctt cgttactttc tccggcggcg cggttcgaaa
    13921 cctctcatcg gtaaaacgga agattcgctg ctttactacg cccctgtatg ggtatattat
    13981 taccggcgcg gctctaaaat tgatctgaaa gttttggacg gttatacggg cagtcctatg
    14041 ggcgggcagg ttcgcaccgc catcgtcaat gcgttcatcg cgcaacgaaa aaggaagcag
    14101 caggagcagc cttcctgatc tgccttttgc ggcacttcct ccaaaaaaga aaactgcggc
    14161 atgtcgcaca gacacaccgc agtcagtttt tgacccgcaa taaaaacgat cagaccaccc
    14221 agggacttct gaactcccgg gtcacccagg cttcatctcc gccgccacgg gcaaactgca
    14281 ttctcctggg gctccatttc agtacttgcc tgtggtaata cgcctgatta agaaggtggc
    14341 acaccgtggc actgcgtccg cccacttcca catcggtgat gggctttttg cgggattcca
    14401 tacagtccag gaaattccgg acatgatcca cactgacata cagtttaatt ttgggatctt
    14461 tcaggaattc ttcttctacc tttgagagtg cagcacccag cgatccttcg tcacccttcc
    14521 ctgcgaatcc ggcgatcttt ttgccctgca gccagaactc aaatttaccc cggttcactt
    14581 tcacttcgcc atcgcttccg tagaaatgca cgccaaagcc tttttgatga atcacacgga
    14641 taccattgtc ataaatgagg ataccgccat ctacagcttc gggattgtcc ggcggaatga
    14701 tctcaaccgg accgctttta tccatatcaa gcccccactg agcaatatcc atatgatgag
    14761 cgccccagtc ggtcaccatg ccgccgccat attctctata ctcgcgccag tccgggaaat
    14821 gtttgtgaat gccccgggga ctgagcacgg aactgtaggg gcgcaagggc gccggaccaa
    14881 gccacatatc ccagtcgaga ccgggttcca gtgtttcttc cggaagatca cagggaactg
    14941 ccggtccacc gaaactgcat tccacccgac tgatttttcc aatcatgccg tttcgcacca
    15001 gttcgcaggc aatacgaaat tctttcatgg agcgctgcat ggaacccgtc tgcagaacgc
    15061 gaccggtcag agcaacggtc ttcatcattt ctttgatctc atggacatta tgagcaagag
    15121 gcttctcgca atatacgtcc ttgccggagc gcatggcggc aatggcaata taggcatgcc
    15181 aatgatccgg agtggtcacg cagacagcat caatatcacg cctgttgata acggcccgaa
    15241 agtcgttgta agcggcacaa tcgggggaac ctgcctccgg atgtccggtg taaaactcat
    15301 tcaccatccg ctggatgtcc agccgccgcg tcgtatccac atcacaaaca gccacgacac
    15361 gggcgcgttt atggttcata aacgcttcga gcagatagcg cccctgcttt ccggcaccga
    15421 tcacggcaat attgatccgg tcattgggct tgtgcgccgc tgaccatatc cgggatggaa
    15481 gaacaaacgg agcagcaccc agcacagaga gctgcgtcag aaatctacgt ctagtgatac
    15541 tcatcaatga attccttcag aatatttctg ctttgtttta ttaaaaagcc agggacactt
    15601 catccatcgc ttttttaaga tgctcaatgc ctttggcaat atctcccaca cgatccgctc
    15661 cggcttcacg ttcgaaaatg tagtcccctg tgaagcccgc ttctgccaga atgcgtacaa
    15721 actccaccca gtcgacttcg ccggtaccaa cgacaacttc ctcgccccag tcgcctttca
    15781 ctttcgtacg gcgggcatct ttcacatgca cctgctgcac atagggcacg aggcggcgca
    15841 gggcttctat cggttcatcc atatcgtaaa ggatcatgtt ggcagggtca aaattcaccg
    15901 acacattatc aacgccccgt tttttcatct cctcaagaaa agccaggagc gattcggcac
    15961 tctcctgccc tgtttccatc atgagcgtca caccatgagt tttgaaaatt ttggcaagcg
    16021 tttccactct gccgcacagc ttgtcataag ccggatcatt tttatcatga ggcaggaagc
    16081 cggcgtgggc attggcggag gtcagtccca tcttttcagc aagagccgct gtcacttttg
    16141 ccagctcctg attctcttcc caatgctgat ccggcaccac acctccggtc acctgaatgg
    16201 ttgcaggcgt cgtgtaatcc tctcctaccg tggagtacat gccggagaca atggaaatac
    16261 cggaagccgc cagaatttcc tgaacattgt cccacactcc gacgtcgccc cgatgcggcg
    16321 taagacccat ctgtactttt ttcagcccca gggcattcac tttttccgcc agatcttcgg
    16381 ggcctgtcgc ctgaagtgac cagctgcaca cgcctatctg ttccgcactt tgctttttca
    16441 tttactattt cctccagggt ttggatttgc tgattggtat tggaatcttc gcttcccaag
    16501 cacggcgctg ttccgacaca tcatggtctc cattgtacca gtacttttat catgaaacaa
    16561 tgcccgaata aaagcagggc attcaaacat ccggagttcc ggctgcttaa aacaggtata
    16621 ataaatgcct gccgccgtga aggtgaaaga atcggaaata aaagggaaat ataatgaaca
    16681 ctgcaggaat catctcccga aggactttta tcaaaaccgg tgccctgtta ggtgcagggc
    16741 tctatgctgc cggaaattca gacttcgccc taagtgcgtc caaaaacaac aggcgcttcc
    16801 atgccagtct ttctattgat gccttggaac gttatcccga tctgctgccg accgtggtca
    16861 atgccggtgt ggatgctgtc tgggtggcgg catttttgca gggaagctgg cagtatcctc
    16921 ctgaaaccgt cggagtttgg aaaaaacgta ttgaagcggc ggggctggaa gtgcatcaca
    16981 ttacggttcc tctcggccat ccgagcttta ccgaagccgc tcctgattat atgggagaac
    17041 tttcttatca accatggcag cgaggcatcc ttccggacgg cagggcgtgc tacggcgtct
    17101 gcattcaccc tccggcgtgc gatgtaaacg cagaaggagt caaaattctg cacgacgcag
    17161 cgcccggcat gatttttctg gacgatgatt tcagacttgc gccgtctccc cacgatatcg
    17221 gcggctgttt ctgcccggca cacaaggcgg catttctcga aaaacacggc tatagcgaaa
    17281 acacgtggga agaaatgctg gcagcggtga aagaccgcag gctgacagcg ccactgcgta
    17341 actggctgga ggatgcctgc accgagcttt ccgattgttt caaggcgcag caggcaatgt
    17401 tgccggaaaa aagcctgggg atcatggtca tgtacatggg ctctgaaaaa gcagggatcc
    17461 gtctggatga ttatcgggat gtccctttca gagttggcga actgcagttt aatgatcagg
    17521 ctttcagtcg ggtcaagggg aaaacggatg aacttttcag tgtcctcttt catcgtcgtt
    17581 ttaccctgcc tgaaaacagc tacagcgaaa gcaccgcctg gccacccgat gggctatctc
    17641 ctgaaaattt ggcggcaaaa ctcgccattt ccaccattgc tgatgtacga aatacaatgc
    17701 ttatgagcgg tctggagccg taccctcttt tctactggga cacgctcgct ccggcaatga
    17761 aaaagcacag agcccttcat gaaacactag cgggacacaa accgactggt cccttgaagc
    17821 attattgggg agacgccggg cgatgggtcg gcgattccaa gccgtacagc ctttttctgg
    17881 cgacgggcgt tccttttgaa gtgacagaaa ttccggcaga tgacggattc acctttctgg
    17941 gagatgggga tgcgaaggcc ttgcaggacg gaaggttaaa gccgggaaaa ggcttatgca
    18001 tcaccagaga aaaagcggaa gcgccggctg acgtcctccg gtacataccg gaggagctcg
    18061 atgctctttt tgctttcaag cgggagcttc tggaaaaaaa ccgggacttc ccttatgtag
    18121 tggaagatct gccggtgatc tgcgcgtggt atccgacggc aaacctggca gccctgtgga
    18181 atctcagtga aaatgcccag cccctgacgc ttgcctatca gggcagggag taccgggtta
    18241 atgcgagcgg tctggagctg gttccggttc cgctgaacgc atgatcaggt cgttctgtca
    18301 gagcgaaaga tttttatcgg taacagcacc ttccgatgct gaagcgcaca gggcagcgta
    18361 ttttgccagt accccggagg tatagcgggg tttgggcgct ttccaggcag cccggcgtct
    18421 ggcaatttct tcctgatcca cttccagcgt aatggaatgt ttcacggcat ctatggtgat
    18481 ggtatctccg tttttaacca gcgcaatcag tccgcctgtc tgcgcctccg gggtaatatg
    18541 tccgaccaca aaaccgtggc tgccgccgga aaagcgtcca tccgtaataa gagcgacctc
    18601 ttttccgagc ccgcgtccga tcacggcgga agtaggcgcc agcatttccc gcatgcccgg
    18661 cccgcctttc gggccttcat agcggatcac aaccacgtcc cctgctttca cagttccatt
    18721 cagaatattg gcaagtgctt tttcttcgct gtcgtacacc cgtgccttgc ctgtaaaacg
    18781 cagtccttca tgaccggaaa tctttgcgac tgccccttct tccgccagat ttccgtagag
    18841 gatggtgaga tgtccttctg gtttgatggg ctttttaagg ggttgaataa tctgctggtt
    18901 tttcggatag gactttgttc cgcgcaagtt ctgcgccagc gtcttaccgg tcaccgtcat
    18961 gcagtcgcca tgcagcaacc ccgctttcag cagttctttc agcagcggtg tcaatccgcc
    19021 aatatccact aaagactgca tcacataatg gccgcttggc ttcagatccg caagtacagg
    19081 cgtctttttc ccaatacggg tgaagtcatc gagggtcagc ttgatcccgg cagtgtgcgc
    19141 aatggcgagc aggtgcagca cagcattggt cgagcctcca agcgccataa ccactgtaat
    19201 ggcgttttca aaagatttct tcgttaaaat atctttgggg cgcaggttgc gtttgatgca
    19261 ttccattaca gccgcaccgg cacgacggca gtcttcgttt ttctcgggag acacggcagc
    19321 ctgtgctgag ctgttcggaa ggcttagacc cattgtttcg atggcacatg ccattgtgtt
    19381 ggcagtatac ataccgccgc aggctccggg tccgggaatg gctttcttct cgatgtcaag
    19441 cagttccgca tccgtaattt cgccgcgggc atggcggccg accgcctcaa atacagacac
    19501 aatgtccgca gcacagccat tcacgcagcc cggtttgatc gtgccgccat aaacaaatac
    19561 ggaaggccgg ttgaggcgaa tcattgccat gacagcgccg ggcatatttt tatcgcatcc
    19621 gccgatggcg accagtccgt cgaactgctc acagccgaca acagtctcga tagagtcggc
    19681 aattacttcc cgggaaacca gagaatattt catcccttcc gtgcccatgg aaatgccgtc
    19741 ggaaatggtg atggtaccga aaataacgcc ttttccgcct gcttcattca cgccggcttc
    19801 cgcctctctt gcaagggcgt ctatatgcat attgcagggc gttaccatgc tccaggtcga
    19861 agcgataccc acctggcttt ttttaaagtc ttcatcagaa aaaccaacgg cacggagcat
    19921 ggcgcggctg ggcgctcttt cgctgctttc cgttacagat tttgaaaact gcctgtgacc
    19981 gcttgggttt ttgatactca ttgctccggt ctccttatag tttttatcat cagtaaagtg
    20041 ctctcgtgaa gaacaataag gaaaaacaat agaaagaagc ggcttcttcc ctgcacggaa
    20101 tttattttct ctcagagctg ttcaaaacag tagtgtccgt gaagtgggcg tgacatgaaa
    20161 actcatcggc ataaatgcat atgctgatga gcatcgggtg cattgcgcgc ggaaggaaaa
    20221 ctcacttttc gccagcccgg tatatttcaa gtctggttcg ctgctcggaa ttgcctgcaa
    20281 aaaaagggat tgaatgttct atactcgtaa tttgctataa tcgtattttc aaatttattc
    20341 agttttttag ataacaggac aagttatgaa ttttcttcag gctttggcac ttcgcgcacg
    20401 tgtatattac tggatagcaa cctggaataa aaaggatctg cattatccgc ctcccgtaaa
    20461 agacaacccc aaattcatga ctgcctggga agcggtcaaa atgattcctg acggtgccac
    20521 catcgccatt tcaggcatgg cgggcaatca gcgctgctct ctgctgctat ggggcatccg
    20581 cgacatgttt cgggcggagg gttctcccaa agatctcact gttttgtgcc tgggtggcca
    20641 gggcggacgg ggcaaagtgc cgggcaccat tgaagaactt gccattgaag gcctgaatac
    20701 ccgtttgatt acaggccaca cagaaacctt caaagggcag ttgaaactcg ctcacgaggg
    20761 aaaaattgaa ctgcaatgtt cgctgattgg aatgatcacg cagattttta aggctcagtc
    20821 agaagggcgc aattatttta ttgcggaaac gggaaccgga acttttgtgg atccccggac
    20881 aggccacggc accccggtct ggccgcctga tgccgaacag tttgtggaag tgctggaaga
    20941 cggacgtttg aaatacacgc ttccgcttat tgacactgcc attttcagcg cccctgctgc
    21001 cgatcgtaaa ggtaatattt acttcaaagg atcgagtgtt ttaggtgaaa gtgaaagtat
    21061 cgccaaagcg gcaaaatata atgggggacg cactctggtt aatgtggggc gtatggttga
    21121 agaaggatac gacgaccaat ttcttcccgc tgagtatgtg gacgctgtcg tttactggcc
    21181 cggcaccgaa caggccgcca ctatcaagca taaaaagtac tggaactgcc ttacacccca
    21241 cagcactatg tccacagaag aaggtctgaa gcgcgtgcgg ctggtcaata agactctgcg
    21301 tgtcatgtcg acgcggacgc ctattgatga tgtgcttgcc cgtcttgcga ccaaagtttt
    21361 tgccgcgcat gcccgtaaag gggatattgt tgatatcggt atcgggctgc cggaagaggt
    21421 ggcgcggctg ctctatgaaa gcggcgtgat gaaagagctg aacatgatga ccgaggcggg
    21481 agctatcggc ggtgtggcag ctcccggtgt cttcttcggt gcagcggtaa acccccgcga
    21541 tctgataccc tctgtaaccg cttttgagcg gatgtacaac cggctcgact gggtgatcct
    21601 gggcatgctt caggctgaca gtgaagggaa tgtcaatgtc tccaaacggg gggaaggtcc
    21661 cctcaaccat gtgggtccgg gcggtttcat tgatttggtg acctgtgcca actcgattct
    21721 cttctgcaca gcgtggggcg cccgtgcccg ctttgaaatt aaagacaata aaattaccat
    21781 cctgaatccg gggaaaccca agtttgttgc caaagtggac gaaatcactt tcaacggcaa
    21841 agaagcgctc aaacacggga aaaaggtcta ttatgtgacg caagtcggcg catttcggct
    21901 gacggaacgg ggaatggaac ttttttacgt gatgccgggc atagacattc aaaaagatat
    21961 cattgacgca tgtgaaatga agattgtgct gccggaaaac ggtgaagtac ctgttgttgg
    22021 cgaagatgta atcacaggca aaaacttcca gttccagtta cagcaataac cgggaactga
    22081 cctctgctcc tgcagaacag cacttttatt tcacctttgt ggttatgaac cacaacaggt
    22141 tgcggattac aaatataaca ggagtccgcc atgaatctgt tcagcaaaat tgctctttat
    22201 gcccatgtgc tgcgctggcg cctgatgtgg aatcgtttca atactcattt tccgccgccc
    22261 gactttgaca atccgaaatt catgacagcc tgggatgccg ccggacttat cgccgacggt
    22321 gccatagtcg gcacttcagg aatcggatcc aatcagcgcc cttccgtatt gtaccgggcg
    22381 ataagagagc gttttctgaa aacggggcat ccccgggatt tgaccgtcgt cggcattggc
    22441 ggtcagggcg gaagagggat cgttccgggg accgtggagg aactgggcat tcccggactg
    22501 aatacctgcc tcattacatc ccatacggaa atgttcaagg cgcaattgcg tctggcgcag
    22561 gaaaatcagt tggaactgca atgcagtatc ctgggcgcca taacccatat cctgcgcgca
    22621 cagggcgatg aaggcatcaa ttatattatt cgggatcaca cgggcgccgg aaccttcgtc
    22681 gatccgcgca ccggcggcgg caccgctatc tgtccccctg atgggcggca gtttgtggag
    22741 gtgaccgaag acggaaaatt gaagtacagt cttccgttca ttgacactgc tgtatttaac
    22801 gctgacgccg ccgatcggca tggcaacatt tactttaaaa actcgtcggt catctgtgaa
    22861 ggattttctc tgcccaaagc ggtcaaaagg catggcggaa aagttattgt gaatgtgggc
    22921 acacttgtag acgaggggta cgacacgaat tttgtcccgg cagagatgat tgatgcggtt
    22981 gtttactggc cgggaacaga gcagaccggc accgtgaagc agaaaaaata ctgggacttt
    23041 tttactgtaa acagcaggac gtctactgaa gacgctctgg cacgggtccg ccagatcaac
    23101 gctattctca aaataaccgc caaacgctat cccgttgatg atgtgctggc gcgccttgcg
    23161 acccgcattt ttgccgctca tgcgcatgcc ggtgaccacg tcgatatcgg tttcggtctt
    23221 ccggaagaag tggcgcggct tctttatgag agcggcatca tgaaaaaact tcagatgatt
    23281 aatgaaagtg gtgctttcgg cggaatttcc gctccgggca tcttttttgg cgcttccatc
    23341 aaccccgatg aaattacaac tacggctgaa actttcgacc ggctgtacaa acgcctggac
    23401 tgggctattc tcggggcgct gcaggtggac agccggggga acgtcaatgt gtcacagcga
    23461 ggtaaaggtg ccatgaacca tgtaggtccc ggcggcttta tcgacctggt cacctgtgca
    23521 aaaaatatcc tgttctgctg cgctttcggt gagcattccg atgtgacagt tcagggcgca
    23581 aaactccggg ttggcaaccc gggcaaggtg aaattcattg acaaggtgga tgagatcacc
    23641 tttaatggtg aagaagcact ccggcaggga aaaaaggtat actatgttac ccatctggct
    23701 gcttttcatt taacagcacg gggcatggag ctgactcaca tcatgcccgg catcgacatt
    23761 caaaaagaca tcatagaaag aagtagcatg cggattctgc ttccggaaaa taaagagccc
    23821 gagatcgtca gtgaagaagt agtgaccgga aaagactttt ccttcagctt gtcctaaaac
    23881 tgcagcgaac cttccggatg tggatttcta attatgcttt ttgcccgaat acatactacc
    23941 ctgccgttca ctctgtcttt acttcttttg tgcctgtacg gctgttccac aaactcagcg
    24001 tctgaaaaaa ctgttcgcaa gtcctctccg cccgctgtat cagccaatac acattttcca
    24061 cgggtcacac tggagcactc tcctcttcct gcaagcgacg cctttcgcct cctcggtgaa
    24121 aaggcaggtg gcggtttcgt gctgatggcg gggcttgaag aacgtccggt acccgccgcc
    24181 aactggcaga aacagcccta cggtgatgtt gtaaatactt atgcccagga gttggactgt
    24241 ctgcttttcc cggcttccgg gtactatttt atcgcaccgg aaggctacga atctctggcg
    24301 gctgtttcgc tcagcgcagc gcttcctgag cgatatcgta ctttaaccgc ttccctcact
    24361 ctgggcgcaa aaacaaagct gtacaccgct tttgcactca tcagccaggt gctgggcatt
    24421 accatactgt ctgataattt tattgcggaa tccccctgcg gggaacttca tcttgaagac
    24481 ctgccactgg gcattatgct ggaagccttg cttcagtcgg cacgtatcgc tcctgattca
    24541 atggctctgg acgcgtcgga cgaatatctg tttattcaag ccaaagaaaa cattcagccg
    24601 aaagaccttc tattaaatga ccgttctctt ccggcagaaa gctcagcaat tcttgataag
    24661 cgccttcccc gcttcgcaat acctccggtc agtcctgaca acgaggcggc gcttttcggt
    24721 gcggaatcca tacccctcaa agatgccctg cttcccttga gcactcaggc cggtattgaa
    24781 atcgtctgca ggcgtgcgct cgcagacatc cccatcaatc ccttcgtatg cacggacgtc
    24841 tcacttcgaa cagcgttgaa tctgctcatc cggcaatggc cactcccctc tttcggatat
    24901 gaagttcagg acaaccgtat tcttctgagg gaaagagcag aaccgtaacc ctcttgtccc
    24961 gcaaaacatt ggcaagcctt caaattccgg tatggccgaa gccgccttcc ccccgctctg
    25021 tttcagaaag cacatcaacg gggcaccaca cagcctgcac aaccggtgcg ataaccagct
    25081 gcgcaatacg atctccccga tgaatcacaa agggttctgt tcccagattg atcagaagca
    25141 cctgaatttc accccggtaa tcagcatcaa tggttccggg cgcattgatc atggttatgc
    25201 catggcgaat ggcaaggccg ctgcgtgggc gcacctgtgc ttcatatcct ggaggaatgg
    25261 caacagtcat gccggtaggg atggcgcggc gttcaccggg ttgaagtgtt gcgtcactgt
    25321 ctactgcggc gactagatcc ataccgctgg aaaactccgt ggcgtaaacc ggcatcggaa
    25381 gatcttccgt tccgggcagg cggtgaacct gtatctggat ctgccgactc gtcataaggg
    25441 aatcccgtcc ggtatatggg tacctttcgg cccaagaaga agcagcgcac aggcatcctg
    25501 ccggatatag cggtttgcga agtcaagaag tccttccgct gtaaccgctt ctatccctgc
    25561 gacaagttcc tccattggaa taatctcatt tctgaagaga aggtttttgg caacccttga
    25621 tatccgggaa tgggtgcttt ccatcgccat cagaagcgat cctttcaact gctcccgaca
    25681 gcggttcaac tcctcctgag acacaggaga ggaacggatc agatccagct ctttaaaggt
    25741 cagatccaat gcctgcgcac agttctgggg agcaaccgct tcataaatcc ccatcatacc
    25801 tgccgggacg tagctgctta tgtaggcaca gacattgtat gccaggcctt cttcttccct
    25861 tattttttca aaaaggcggg acgtgctgct gccgccgaat atgccggaca gcatgctgcc
    25921 tgcataccgt tcctgatctc ccatagaggg ggcaggggtg gctataccga catgaacctg
    25981 tgaaatcggg cgttctacca gtttggtccc tgcctgaaaa cggggaaccc tcaggtcgct
    26041 ttccggagta ccggcaggaa gcccactgaa gatttcttcg acctttgcca ggacgcgatc
    26101 ctgatcaaaa tttcccgcaa tagcaaaaac catattctcc ggcttatacc aggcatcgta
    26161 aaactggagc agatcctgtc tgcttaaagc agccactgtt tcagcatagc ctgaaacagg
    26221 ccgcccgagg gaatgatcag gccaatgaaa ttctgccagc agatcgcttg ccagttcatc
    26281 gggcgtatct tcgatagaag caatttcttc gagtacaaca ttgcgttctt tttcgaggtc
    26341 acaaaaaagg gaattcagaa gaaggtctcc aagcacctct gccccaattt ccacatgctc
    26401 cgccagcata cgcacataaa atgtcgtata ttcctgagaa gttccggcgt tgaaataccc
    26461 gcctttgctt tcgatagcat ccataatctc gtggacattt ctgttttttg tccccttgaa
    26521 aaaaagatgc tccagaaaat gagcgaggcc ggattgtttt tgcgtttcgg acgctgaacc
    26581 cgctttgatc cagacaccca gcgatgcgga atggagatac ggcaaatgct ccatcgcaat
    26641 cctgaagccg tttgggaggg ttacgacccg gatgttttca tcaaaattat tttttgtcaa
    26701 agcctgtttt tcctacaaaa aaaagttgcc gcatgggaac ggcaacttta cataattttg
    26761 aaatcagaaa tcaatcacgg tcggaacgat catttctgcg ccctctggaa gggccgcccc
    26821 gtcgtccgcc atcattgcgg tcacggcgat cgccgccccc gcgaccaccg cggttgtcgc
    26881 gctgttctcc gccaccgcgc tgctcaaaat agccttcggg ggcaggctgc acctgatcca
    26941 attcccgatc tgcggcgatt ttggacaagt tcactctgcc catggagtcg atttcgagaa
    27001 ctttcacatc aatttcatcg cccacattca ccacatccgt cacattgtga acccgataat
    27061 ctgccaactc ggaaatatga accaggcctt ctttacttcc gataacggta cagaaagcgc
    27121 caaagttcat gatgcgcgta acacgaccgt gataaatctt gcctacttcg acttcagcaa
    27181 cgatctcgcg gataaagtca atagcggcgt cggcatcttc tttgttggta gcggcgatat
    27241 taactgtacc gtcatcttcg atttcgattt cggcgccggt ttctgcctga atctgcctga
    27301 taaccttgcc accgggtcca atcacctcgc gaatcttgtc gggatggatg gtgatggtga
    27361 aaatccgcgg cgcatagggc gacatgtctt cacgcggagc agccagggct gattccataa
    27421 tactcaatat gtgggcgcga cctgcccgtg cctgctcaag agcctgctcc atcacttccc
    27481 gggacagccc tttaatcttg gtgtccattt ggaaagcggt aatccctttg gaggttccgc
    27541 agactttaaa gtccatatca cccaaatggt cctcgtcacc cagaatgtca gagagaatac
    27601 ggacttcatt cccttccttg atcatgccca tagcaatgcc cgccaccgaa ttggttacag
    27661 gaacgccggc tgccatcatg ctgagcgagc cgccgcaaac cgttgccata gagctggagc
    27721 cattgctttc ggtaatatca gagacaatgc ggacagtata gggaaaatga atttcatttt
    27781 cctggactgt ttcctcatct ttttcctgca ggaaaggaag catgggttcc agagcacgct
    27841 cggcaagttt accgtgcccg acttcacggc ggccgggacc ggaaatacgt cgcacttcgc
    27901 ccaccgacca ggcaggaaag ttgtagtgca ggaaaaaccg ccggaactca tctccggtca
    27961 gttcatccag ccgcatttca tcccgactgg ttcccagtgt ggtaacgacg agcgcctgag
    28021 tttccccgcg tgtaaaaagc acactgccat gcgcgcgagg cagaatatcc agttcaatgg
    28081 taatctgcct gatttcatcc aaccggcgac catccatgcg gatattggtg tcgattacgg
    28141 cctgccgcat gatccgtttt ttcagatcgt tgaaaatttc tccggcggca gccccaaaag
    28201 tcgcacccca ttcttcgccg tatttttcga gaagcgttgc ttcaatttca gattttaaag
    28261 catcaatggt atccagccgc acttgctttt ctttgatggc cagggcatcg cccagtctct
    28321 tcgcagcaag cgtttcaatt tcggcgacca gttccggcgg ctgctcatcc tcgggcaccg
    28381 gcattttttc cacaccggcg gcttcccgaa gcgcatcgat ggcagcgata atattgcgaa
    28441 tatgcccgtg tgcaaattcg agcgcctcaa ccatgaaaag ctcagatact tcgttcgcct
    28501 gaccttccac catgcagatc gaatcacggg ttccggcaac aacaatatca agcgtgctct
    28561 tcttcatctc ttccatggct ggatttgctg tcagcacgcc atccagacaa ccgaccctga
    28621 cagcgcccac gggctcaaga agggggattt tcgaaatgtg cagggctgca gaggcagcat
    28681 taatagagag cacatcagga ttgttaacat tgtctgcgga aaggacggtc tgacaaacca
    28741 tcacctcatt gaggaagttt ttaggaaaga gggggcgtaa ggggcggtct gtcatacggc
    28801 agaccaaaat ctcccgctct gacggacgcg attcccggcg gaaaaaattt ccgggaatct
    28861 gacctaccgc atagaatttc tcacgataat ctaccgttag agggaaaaag tcctgaccat
    28921 cccgcatatc cggcgccaca gtcacggcgg aaagaaccat cgtatcaccg ctgcgacaga
    28981 ttacggcgcc gtgcgcctgc ttggcaacac gccctgtctc aaacgtcatt ttttccgtgc
    29041 caacatccac tgatgctgtt tttaacatct tttttcatcc ttttgcgact tcatcgcttc
    29101 tttagtaata ccggctgaac caatcgccgt ttcggaggca tggaggagac gcatgagacc
    29161 ccgtacaatt caatacccgc atcggctgcg cacattctcc tgaaggagtt acttacgcag
    29221 gcccaattta gcaatcagcg cacgatagcg ctctacgtca ttattggcga cataggtcag
    29281 cagattccgc cgcttgccga ccaggcgcag cagcccgcgc cggcacgcat gatccttggg
    29341 atgctggcgc aaatgttcgt tcagctgatt aatcctctcg gtaaaaatag ccacctgcac
    29401 ttccacagag ccggtgtctt tatcagaacc gccatgttct ttaataagcg tttgtttttc
    29461 ttcttttttc nnnnnnnnnn gacattgaaa ttcacctttc ctgttaatcc gcccgctcca
    29521 ggcacggcgc cggtgaaccg tccggccgag tcgcgggtaa cttgtttgaa gaccctttca
    29581 gatctcttta tgcattcagg ccttatgcct ttattttcag taagtaaagt ataccatgtt
    29641 tccgcggtct gttacgattt ctgacaggag caacagccgg aacagctccc ggattcgggc
    29701 ttatcggaaa gagctgccag accgggaagc gttttccctt ccagcatctc cagagaagca
    29761 ccgccgccgg tagacacatg agacattttt tcggcaagtc cgaattggat caccgctgcg
    29821 gccgtatcac cgccgccaat aatgctggtt acgccggggc tctctgccag cgcatctgca
    29881 acagcacggg ttccttttac aaaggagggc atttcaaaaa cgcccagagg cccgttccat
    29941 accactgttt tggcttgctg aatgacgtcg cagaaaagag cgattgtctt cggaccgata
    30001 tcaagccctt cccaatctgc cggaatggca tcaatgccta caatctgtgt gttggcatta
    30061 tcagcaaaag catccgccac aacaatatcc acaggcagct gcaatttcac gcctttttct
    30121 tcggcaagag cgagcacttt tttcgcaaca tcaattccgt ccttgtccag aatagatttg
    30181 ccgatttcat aacctttggc cttgaggaag gtataagcca tgccaccgcc gatgaggagc
    30241 gtatcgacga gatttaaaag attttcgatg acagtgattt tatcggacac ttttgcgccg
    30301 ccgagaatag ccacgagcgg gcgttcggga ttgtccagca ccttgctgaa gtactcaatt
    30361 tctttggcaa ccaggaaacc ggcaacactt gtgggaaggt aatgcgccac gccctcggta
    30421 gatgcgtggg cacgatgcac cgttccaaac gcatcagaga cataaagatc tccgagtttt
    30481 gccagctgct gcgcaaactc cggatcattt ttcgtttctt ttttgccgta gaaccgtgtg
    30541 ttttcaagaa gcaccacatc gccggggttc atggcggcaa tagctgcctc cacctcaggc
    30601 cccacacagg catccagttt tttaaccggc gcacccagca attcggacag tttcgctgca
    30661 acaggatcca ttttcagcaa ctcatttttg cgctgaatct cgtccttttc ttcctgcgtc
    30721 tccgcctttg ccacatcgcc ggggcggccg agatgggaca taagtacgag tttgccgccc
    30781 tgatcccgca catattggat acttttgagt gcggcacgga tgcgcatatc atttcgcacc
    30841 gaaccatccg cattttgcgg cacattaaaa tcaacacgca tcaaaaccgt ttttccggca
    30901 tgagcaactt catcgattct aagtttgttc attgcagtat cactttccct atgctttttt
    30961 gtaagaatcg aggccgccat tgctgtgcgg cctcgataga aaattacatc acaacagaat
    31021 caggacgcca tcattttgaa gaggtccaga cagcggttgg aataacccca ctcgttgtcg
    31081 taccaggaca cgatcttaat gaagtcgcca cccatcacag tagtttccag cgaatcgaag
    31141 atggaggaat ggggattgcc gataatgtcg caggacacga tgggatcggc ggtgtactga
    31201 agcacgcctt tcagcgcacc ttcggcagca gccttgatcg ccgcattgat ttcatctttg
    31261 gttgccggtt tttcgagctc agccaccaga tcaacaacgc tgccgttgat cacggggaca
    31321 cgcatcgcca taccgtcaag tttgccgtta agggcgggca gaacctgacc gacggcacgg
    31381 gcggcaccgg tggaggtggg gataatggaa tgcgcagcgg cacgggcacg gcgaaggtcg
    31441 ctgtggggca gatccagaac agcctggtca ttggtgtagg cgtggatagt ggtcatgaga
    31501 ccgcgcttga ttccgaagtt ctcatggaga actttggcta cgggagcaag gcagttggtg
    31561 gtgcagctgg cgttggatac gatacagtct gtgctcttca gcgtatcatc attcacgccg
    31621 actacgataa cggcgtcaat agcatccttc ggcggaacgc tgagaagtac ttttttggca
    31681 ccggcctgaa tgtgcatgtt gcactgttct ttgctgcgga aaataccggt agattcaagc
    31741 acgagttcca caccaagttc accccaggga aggtcggcag gattgcggac tgccagcact
    31801 ttgatttctt ttccatcgac tacgatggca tcgtctttcg cactgacttc tttgccgtac
    31861 acgccgtgga cactgtcgta tttgagcagg tgcgccagcg tcttggcatt ggtcaggtca
    31921 ttaattgcca ccacttcaaa tgcggggtcg gcaaggagca gtttgaacac gttacggcca
    31981 atgcgcccga atccattgat accaacttta gttgccatgg cttgttcttc ctttcatttg
    32041 tatggacagt caaacacata agagtccagc tgccacgtgc cggagtcttt ttattaaacc
    32101 acttttttgc gcatcgcttc cgatgtgtta tgaagtggca tcactaaaga tttcaaaaaa
    32161 acctgctata cagatcactg catgagacag gattttcccc cggaagcaaa attcttgctt
    32221 tcaagaccta aaataactta ggttattatg tgtagtatgt cataatccgg gttgacaagt
    32281 caattttccc tgcctcggac agactgccgg cagcaatctg cagcatatac ccgcaggctc
    32341 agcattacag atggtaagga tgccaatcat gcgaaccaga tgcctcagac ctttgctctt
    32401 tttttgcggt atcgcaagtc tcgctgtcat ggtgagcgcc tctttgcaag gctgcggatc
    32461 ctgttttggc tccaaaggca cttccggcga ccctgccagc cagacccctc actcaccccg
    32521 tggcgcacga aaccagtcgc cggtctcgcc tcgcgcatcc cgcagccaga caccgcaggc
    32581 accgggcgct ctcgctgtgc ctgccgtaga gccggcagtg cttcccgaaa ctcctgagct
    32641 gagtattcag acagctgggg acattaaacc gggtatgact tatgaggata tccattctat
    32701 ccttggctct cccggcgtag tgatagccgg gaccgatcag gacaatgtag tttaccgttg
    32761 gagctatgcg ggcatgagtt tcatggggcg ttttgaaaat ggcatccttg tccgaaaaac
    32821 agccgtgcaa aatgacgacg agtaccgcgg gttacaggat gaggatattg tctcttttga
    32881 caaggtgctt tatgaaaaaa ttcaaccggg aatgagctac gacgaagtgc tcgctattat
    32941 tgggatggat gcgcaaccgc tgacaagtaa ctccgggcct gtaaaaatct acaaatggac
    33001 agatgagcgc ggttccagca tcacggggcg cttcgaaaac tcaattttaa aacgaaaatc
    33061 aggaatgatt ctggagccgg gcaaaggaga gggagaaatt gaggttgcgc cttctcctcc
    33121 tgccggggca gtgcatgagg ataaagaacc cgcccgaatc tctgaaaaag aatcggttcc
    33181 gacatatgag gaacaggagg aaagcactgc agaagtgagc agctcagaca cggagggaac
    33241 gcctgctcag gaaaaaccgt cccgggtcag cgtgtccggc tcttcacggc ggagtcgaac
    33301 gcagggttcg gaagccggtg cccgtgcttc tgaacgctct tatcacccta aagtaaaact
    33361 gccggaattc aagcggcggc ttcgggaagg tgtctatgaa atccgcatcc acaacacgac
    33421 ccagtcaaac ttaaaagttg ccctgatttc cgaggaaggc ggcctggaaa tgcggcttgc
    33481 ccccggaaga aattcttccg ctaaagtcgg tcagggtacc tatcaggttt attttatcaa
    33541 cgaaaatgat ccttttaccc ttcatcaggg acaaagcatt cccgttgccg ccctgttatc
    33601 cgacttttca gtctatcttt tcgaagacac ttacgaagtg gatttccttg atcggaatgc
    33661 gccacgctcc ggaaaccggc gttagtttga gcgccgtgcc gggatgcata ctgcctgaat
    33721 gaaacctgaa agcccgcacg gaagtcttat gttcaggtca gggcagttga aatcattggt
    33781 aacaggtgtt ttaatgcagc gtcttttttc ttattacccg ggcgcagcgc catttatatt
    33841 cttcaaagca agcacaactc catgaaaaaa aactttacca tagcggtcat gattgccgtg
    33901 gcaatccaga ttttcagtgt gggcattctt gcgccacgac taccccggga agtctgccgc
    33961 cctgaagccg ctacggagcg ttttttctcc cttgaaccgg cgaccgcatt ccgcctgatc
    34021 aacgccgatg gcagcgctca aatttcttcc tcggtttcgg caaaaaaaat agcggtcaaa
    34081 gcacggatcc gtgcctatac cgccaccgcc gaagggcagg ttcttctgga aaaatacctg
    34141 tcatcccttt ttcaaattca ggaaacgcca gaactgacga cctttacgac tgagcccatt
    34201 aaacggccgg atcctattga tcttcgtgtg gattacacca tagaagtccc tcaggacacc
    34261 gatctgtctc ttgaagtctc gaacggcaac atctgggttt ctcctggcgt ccggaacgta
    34321 tccataaccg cgaacaatgc ggatattcaa gtactcgaag cccggggtga tctgagcata
    34381 aaaaccatta acggcagaat tcacactgaa aaaagttgcg gtgaaagtac gctggaaacc
    34441 attaacggaa gtatccagac aagtctgaca tcgggcactt tgcaggcctc caccacaacc
    34501 ggaaatattc tcacaacgct tcttgataat gaaatcacta cctgcgactt gacctcactg
    34561 aacggaagta ttacactagt catgccggaa aatccctctg cagaagtgaa tgctgtcact
    34621 ctgcgcggga tcattcgtgc cgatctgccg atggttcttg cgggcggacc taaacgccgt
    34681 gagctttacg gaaccctcgg agacggctac acaaatgtgt caatgaattc catgaacgga
    34741 agcattgtca tacaaaggaa tacgccatga ctcgtgaagc agtgttagat accggattag
    34801 ccgtcaatgc cgattcgctc gaagaagtca gcgacataga gctggtgcgc agctgccagt
    34861 caggcagtcg cgaagccttt tcggaactgg ttcgtcggca ccagcatctg gtgtacaacg
    34921 ttgcgtaccg gtacatgcgg gatacaagct ccgctgaaga catggcacag gaagccttca
    34981 tcaaggcata tcggctgctc aaaggctttc gtggtgactg cagttttgcc acctggatgt
    35041 accgcgtcac ctccacgacc tgcctcaccg aaatcaataa aaagaaaaaa cgcaatgaaa
    35101 tagcactgga agcccgggat tcggatttga tcgccgacaa ggcgccgctt ccggaggaaa
    35161 cggacatgcg ggaaagaatc cgcaagtgtg tcacccgtct tccaaaccat tacgcaacag
    35221 cgatcactct ttattatctt aaaggggtca gttatgacga gatcgctcag gcactggaaa
    35281 ttcctctggg caccttaaaa acctggatgt tccgtgcacg cagacaattg cgtaaaattg
    35341 ttgagaagga attgcgctcc tatgaatgag cactttgact gccgtgacga tctggacctt
    35401 ttgattgcac aggcgctcgc tgaagagcct gtgcttcagg caccgccttc gctgcttgaa
    35461 agcatcgagg cacgtctgga tattgcggaa ctgcgtgcgc atgaagaaag gcgcttccgc
    35521 atttccatga cttctcttct ggttattttt gtcgcggcac ttacagtcgc cggcctcagt
    35581 ctgtggttta ccaatctcag tgtgctgtac agtgaaggtg tttccggggg taaaggtctg
    35641 tttgactatt atctgactgc tttccgcatg tccttctcat cgtaccaggg cacctacagc
    35701 ctcgcgggcg gattattttt cagcatcagc gctgttgcaa ttcttgcagc tttgcagatc
    35761 cataaaatgc ctcattcgga ctgatccgca ccaatcagcc agccgaaatt ccggcgcaaa
    35821 agggtttcca gcggcatgca ccgtgttaca gtggctactc ttcttgtcat gtctcccttg
    35881 cttttcgccg tatcgacagc tgcacccaaa gcacccgctc ccctaaagcc tccctcccat
    35941 gcgtccgggc aaatggagaa agactctttc tcggagactc cgagtcctga aaaggttgac
    36001 agcactgcgc ccgacaaggc gttgcctatg cctccgccgc cgcataaaag tgccgcctgg
    36061 gtgctggaac agatgagccg tgccgataac ccggaattcc gacggcgcgc ttctgaagcc
    36121 tggccacttg atcctgatgc gctggaaact ctggagcctc ttatacggca attatccgac
    36181 ccaagtgccc aagtgcgctc cgctgcggag ctcaggcttg cggaatatcc ggtcccaacc
    36241 ctttttgcct atgtcatgcg gacattcact tcattagaca cgcagaaaac cgcatggctt
    36301 gaggtcgcgc ttccttcact gcccgctgct gcaggcacct actttttaga aactctgaac
    36361 acggaaattg aattgcccct tcaccggcgc atcgccgcct atgtcctcgg gcgcatgcaa
    36421 tatcgggctg cgctgcctac gctgacccgc cacgtctgga gcactgatat ggagctggca
    36481 ggcacctgcg tggaagcact ccgttccctc cacgatccgc agagtctgga cttgtggatc
    36541 cagctgctgc agcacgacaa tgtctatttc aggccaatcg ctgtacaggc gctcgccgca
    36601 ctgcgcagtc cggccgcact ggaaaagatc agacaacttg taatccatcc ggggaatacg
    36661 gcagcccaac tgagtgcatt aagcgctgtc gaatcctacc cgccggaagg acagctgccc
    36721 atactgattg aaatattgga gaaaaacccc gaactcgccc cccaggcgca ccggatgctg
    36781 agaaagcagg cgggtatgga tctgggcacc caggccggtc cctggcgcca gtggatgaat
    36841 acgcttttga cgccgcagcc cccgccgctt gtaccttccg aataaattga attatctgcc
    36901 cgcctcaccc ataatataaa cggcagcaac ccagcaagga gtctgacaat gcgtatcatc
    36961 agtgtttttc cggcgcttgt tctggcaaca gccagtctgt ctcttttatc ctgtgccgat
    37021 gatgtccttc gtcttccaac aggcaaagca acagaagccc cttccggcga agactggatc
    37081 gatttgtttt ccgcagaaaa tgcaccgctc tggaaaaacg tgaccgactc tgcttctacg
    37141 gcctttaccc tcacggaaaa cggcatgaaa atgaacccgc aaaaagagac gcgctatatt
    37201 gcgtggacgg gcggcagttt ttctgatttt gaattgcatg tggaattcaa gtgcggcacc
    37261 gatgccaaca gcggtgtttt tttcagaacc gatcctgccg acccggtaca gcgcggcatg
    37321 gaaattcagg tcttcgggga tcatgggcgg catccgtccg tgcatagcag cgcggctctt
    37381 tatgacattg ccacaccggc ctttaatatg gcgctgcctc cgggcgagtg gaattcttat
    37441 gacatatcag ttgacggtga tcaggtcaca gtaatctata acggctggaa agtgctggat
    37501 ctggatctcg ggctcatgac catgcctata ggcaaatttg atacgcctct ggcttcattg
    37561 cccaaagagg ggcatatcat tctccagaac catggcgatg aagtttggtt tcgtaatctt
    37621 aaaatacgcc cgaagcaata aagtacgtac tcttttgttc ccatatcgac tgcagtcatt
    37681 cagaaataga gataagggtg atatacatct atgattctga cccctgatca acaggactgg
    37741 ctcaggctct ttcttgtgcc cggcattggc tcggtgcgat ttctcaaact gctggcacac
    37801 ttccagaaac ccgggagggt gctgcgtgct tcggtaaaag agctcagcga tgtaatcggg
    37861 aaaactgtcg ctcagcgtat ccgtcagcat gcggacgccg ttgatatgga ataccaccaa
    37921 taccagatga agacgcatgg cgtctccatg gttaccatgg atgatcctgc ttatcctgta
    37981 tctctggcgg agacctatga tgcccctgtc ctgttcttca ttaaaggcac actccttgag
    38041 tctgaccaga aaagcatagc cattgtcggt acacgacgcc cctcctccat cgggttgaag
    38101 accgctcacc agctggctta tgaacttgcc gcccggggca tcactgtcgt aagcgggctg
    38161 gcggagggca tcgatacagc cgcacataaa ggtgcccttg atgcgggtgg cagaaccatc
    38221 gctgtgctgg gctgcggtct ggacagcatt tacccggcat ccaacaaaac actcagcgaa
    38281 gaaatcagcc ggcaaggctg tcttttgagc ccctttgtca tgggtacccg tgccattcgc
    38341 ggcaactttc cccgccggaa tcatattatc agcggattga ccatgggcac gctggttgta
    38401 gaagcgcctc tgggcagcgg atccctgctt acggcaaaat atgcggcaga gcagggcaga
    38461 gaagtctttg ccgtgcccgg cagtgccggg ttctccaaca gccagggtcc caatgatctg
    38521 attcgtgacg gtgcacggct ggttgaaaaa gtggatgata ttcttcttga gctgccgctt
    38581 gctgaaaacg aattttcgac ctttaaaggg ctgcctgaaa gacctgtgcg acagccgtct
    38641 ccgccaaaaa tgagtcctgc tcctgtagcg aaaaaagaaa aaccttctgc cccctctccg
    38701 aatagcgaag caccaccaaa agcgctgtcc ctctcaaagg aagagcaggc tgtttatgat
    38761 gtgctgcatc aggaaggctc ttttgtggat gagattgctc ttgccacacg acttgctgtt
    38821 ccggaaattc tggtctcgct gaccatgctg gaaatgaaag gggtggttag gcagatgagc
    38881 ggaaaacggt ttgcacgaaa atgaactata ttgatgcact ggctcacaaa atacgaaagg
    38941 acgagacact gccgctgctg ctttcggttt tactgcgggg aggttccgtg ttccagcgtc
    39001 ttggcatgtg gaagcgcctt cattgccctc gtgtgcgcgt ggccgccaga gtcatcagct
    39061 tcggtaatct caccgccggc ggcgcaggca aaacgcctgc cgtcatcgag cgcgccctcc
    39121 gtgaaatcag cctcgggcat caggtagcag taatcacccg gggatatggc gccgctcgcg
    39181 cccggaaagt gcaaatcctt ccaccgggtg ccactgatcc ggacgctgta aaaagattcg
    39241 gcgacgaggc tcttctgatt gcacgccggg ttcccggtgt ttggatagtg cggagcgcca
    39301 atcgggttaa aggtgcacaa gcagcacttg aacagggatg tgacacactg cttcttgacg
    39361 acggatatca ggccgtttcg ctggaacggg acgaaaacat cctgctcatt gacggggcaa
    39421 atcctttcgg gaatggctgc atcattcccc gcggactgct gagagagcct ctgcaagcca
    39481 tgaaccgtgc cacagaaatc ctggtaacac ggtgtgatca gaccacagac gaggcactgc
    39541 gtaaactcac cgaaaccatt cggatatata acagccgggc gccgctctct tatacagtgc
    39601 ataaaccact ggaaattcag cggctttgcg acggcagcac attgcctctt tcctttctgc
    39661 agcagcaatc tgtccaggca ctatgcggca tcggcaaccc ggatgctttt tttcagacgc
    39721 tggaaagtct gggggcgcgg cgggagcaga ccaccgttct cgctgatcat caggcgctgg
    39781 atcaggtggc tttttcttcg gaactgcccg tggttatgac tgaaaaagat gctgtgcgcc
    39841 tggggaaaac agatctcccc aatctctatg cgctgaccgt gtcgctggca ccttacggat
    39901 gaaggcacgc ctccagcgca tccagatagt ctgcgcccgg aagcaattgt tttagttccg
    39961 gcgaaagttt tttctcgtga agggatcgct gcctgaaaaa agcggcaaga agagcagcat
    40021 gatccgccgg aagagccttc attaaagcat ccctgaagtg ctcgggacgg gcgggcgcca
    40081 aaggcagccg atgttgtcct tcgccgaaac ttccaaataa aagggaggcg acgaactgtc
    40141 ccggccgcag ttttttatca agacgacggc acgaagcgat tgcaagagag aaggagcgat
    40201 ccttgttttt tttcacccat aaatcatctg aactcaacag ccggggcgtt acaccttcga
    40261 actgatcagg atcataagcg gcggcaagat gccgtattaa ctcacatttg attttctctg
    40321 tttcctgtac ggtaaaacgc cgcacttcac tgccgcgggg tccccactgt gttgaagaag
    40381 caacatacag gggtgcccac tctcccggca gcctgaaaag ccacgcaaag agttctctgt
    40441 tgatatcccg gcgctgatgg cggtcgggaa gatagatgag gccctgacga aggacgggga
    40501 taaatccgtt gtgtccgctc atacccggtg cctgaaaagc agcatattcc tgttccagag
    40561 ctgcggaaaa ttcttcatgg ttttttgcca tcataaggta caaatttgtg cgtttcccct
    40621 gacggctccc ggcaagcagt gaaaaaatca gcctgctttt attttcgtaa acaagatcaa
    40681 cggcaaggct gtccggtgtt tttccgtcca ggccctcttt aaaactgcga taaagaaaat
    40741 aatctttttc ttccagtttt ttttcaaggg cgcgctggag agtttccata cgaaaaaact
    40801 gcttgctttt tttcggatac ggtccggcat agcgaagagt ttcggaccat tttattttaa
    40861 ttttagacac accctgccgg acagggtatt taggcggaat atcggacttc actgagatat
    40921 cctcatagta ttgaaaagaa aactaccgga gatgatcatt tacattaaag agacaagccg
    40981 gcgcggcacc agctttccgg agcggcagat gagcactttt ataaacgaga ggccgttacg
    41041 cgtttttctt tttggctgca ggcttttttg ccggcgcttt tttcgtagtc gcttttttcg
    41101 ccgttgtctt ttttgccggc gcttctgcgc tctctgcttt acatgatgac ggcgcagccg
    41161 cagacccttt gcactttcct gatgtctttt tcttgccgct gcgctcaacc agtttcaaca
    41221 tcttttcgaa actgctatcc acaacacggg taaactcttt taatcccgaa tccagcatat
    41281 ccgcccactc gtccagacat tctttgcctt cctcaggcat ccactcctgt tcttttacgc
    41341 ggtcccggat aattttgtcg acctgcttat ttgttttgcc gataagactc aagccggtct
    41401 tcattgcgcc ctgctgaatt ttaaggagat gcttggcgct ggacaatgaa atgtctttgg
    41461 atgcggttga gccggagtca atcagatccc ctgccagttt catggaatga gacaccagat
    41521 cgtttacggt atcaaaaatt ccaacagacg acttttttgt tttttcagca ctcatttgca
    41581 tactcctttg gatgatctac cgggcacttt gtttccaatc agataactta atgtctatta
    41641 tacgccctgc cggtataaaa gaccatagag acaacggtta tttttaaaaa ttcctgcagg
    41701 caagaatttt aagcctcttt accctgcacc gctctttttt gttatagtat attcctgagc
    41761 gtatgtagag cccttctcag tatcaaaaga ccaacgctaa aggagatttt aaatgtattg
    41821 caaacagagt gccgccatta cagtgctgat gctgatcgcc gtccttctcg tttccggctg
    41881 cgccaccact gccggaggaa aaagtgatga ggaaatggtg aaggaaactg ccagcctctg
    41941 ggcacaagca cttgtagagc acaacgtaga aaaactgctc tccactattt ctgaatcctt
    42001 cgctgctccc cagatggaat ctgcagacaa agccatgctc ggtaccttca tccagcaggc
    42061 tattgattcc ggatatctgg atgatgctga agtcagcttt gaagatgcgg aatatgagct
    42121 gaaggacggt gaatacttca tttatcccat cgacctcatg ggatcagccg gaagtgtatc
    42181 ggtcgagctg ggtctgaaaa aagaaaacgg cacctggctg gtcagctcca tgagcgtgga
    42241 cggtatttaa ccggactttg gcatagcctg ttttgaagca actgctttcc ttgaaccatt
    42301 gtctttcatt caaagacttt ccgggaattt tcagccatgt ctgcatcttt agatgaagtt
    42361 ctgcaagcaa tacgtaccgg catcccgtta tttctggagc gttttcagga atgcctgcgc
    42421 attccaagca tttctacagc cccggaaaac cagcctgata tgctgcgcat gtcacaatgg
    42481 cttcaggcgg aaatgcgccg tatcggactg gagcatgtgc aggcataccc cacgcccggt
    42541 cagcccatgg tctatggaga ctggattcac gctccgggca gaccgacggt gctggtttac
    42601 gggcactatg atgttcagcc tgctgatccg ctggacgaat gggtgagtcc tccctttggt
    42661 gcggaaattc gcgacgacta tatttttgcg cgcggcgcct ccgacatgaa agggcaattt
    42721 ctcgcacagc ttgctgcggt ggaggcctgg cttgcacaag gcacttgccc tgtcaatctg
    42781 aagtatctga ttgaaggcga agaagaaatc ggatcgccct atctcgatga cttcatcaga
    42841 gaccataccg atctgctgga atgcgatatt gtcctgaact gcgatgccat gatccacagt
    42901 gcggagaatc cgtcaatcac ctatgcgctg cgcggtctcg cctatttcga actggaaatc
    42961 tgcacagccg aaaaagacat gcacagcggc atgttcggcg gagccgtccg caacccgatt
    43021 catgtgctca cggaagtgct cagcggcatg catgatgctt cgggaaggat cacactgccg
    43081 ggtttttatg accatgttcc cactgcggaa gacgatgagc gggcgcttct cgccaaagca
    43141 ccctacaatg atgcgacctt tctgaaaaca gccggtgcct ttgctctgta cggtgaggaa
    43201 ggattcacca cactggaacg tctgggtgtg cgcccttcgc tggatgtgaa tggcatctgg
    43261 ggcggctttc agggcgaagg cgccaagaca gttctgcctg cacgggcagg tgctaaactg
    43321 tccatgcgcc ttattccggg gcagcctctg gacatggttg aaaaacaact gcgcgcttac
    43381 ctggaagcaa aggttccggc gggctgccaa tggaatctga cctgccattc aatcggtccg
    43441 ggttcggtga tgcactggaa atctccctat atgcaggcgg ctgcagatgc cctgaaaaaa
    43501 gtcttcggaa aagaaccttt tctccggcgt gagggcgcca gtgttccggt ggtcagactg
    43561 cttcaggagc ggctcggcgt ggactctgtc atgctcgggt tcggccagcc cgacgacggc
    43621 attcacggcc ccaacgaaaa acagtcactt ccggttctgt ataagggcat ggaaagttat
    43681 gcctgcttcc tctacaacgt gggcaggctg aagccgatga gtgtccatga ctaaagagcg
    43741 gctggatatc cttgtacagg agcgttttca gctgacccgc agtaaagcac aggggctgat
    43801 tcagacgaat tgtgtgcgca acagtgcggg agagattctg aacaaagcgg gcatgcgcct
    43861 ggatccggac acagtacttt ttctgtctga gggtccccgt tttgtcagtc gcggtggcga
    43921 aaaactcacc cgtgcactgg aagtctttcc cgtgtccctc cagaaccgtg ttgtcatgga
    43981 tgtgggcgcc tctacgggcg gctttacgga ctgtctgctt cagcatgggg ctgcgcatgt
    44041 ttatgccgtg gacgtaggct acgggcagct cgcctggtct ctccgtcagg attcacgggt
    44101 cacggtgctg gaacgctgca acatccgaac gctaagaaaa gagcagattc cgctctctcc
    44161 cacttttttc acggtagact gctcttttat ttctctgaag gtggtgctgc cctgtatcgt
    44221 cgacctgctt tcccctgttg tggaaggcat tgtactaata aagcctcagt ttgaagcggg
    44281 tcggcatcag gtgggaaaag gcggcgtcgt acgggatccg gctgtgcata cggaagtggt
    44341 ggagggtatt tcccgcttcg ccgccgggct cggattctcc gaggtcgaca gcacaccatc
    44401 accactgctg ggccccgccg gcaataaaga atttcttgcc tggcttcgtg gctttacccc
    44461 gcaagaaagc atcggtacgt aatcaccgga actcatcttc aataatgtag gcaataaaac
    44521 gctcaatgga aatctggtaa tactggcttt caggggtaat gatcacaccg gtcgttatat
    44581 cttccttgcg ttccagttca tcaatcgcct tgctgagcag atcgtccaca gtttccgaaa
    44641 gatgcttaac ctcaaattta tccatggaac attccacggg cctgttggga aatcccagaa
    44701 agccgaaaac ccgcccggct atctccacat ccacttcttc acttccgaaa atatcttcat
    44761 acttgctctg gtaatgacgg ggtttgataa gaaattgcgg agaaacatga atacgccccc
    44821 tcacatagag actgccccta gtgccgtcca gaccggcgcc cagacaaata tacggcaact
    44881 catgataacc ctttacgatg ccgtaggtag gccgtcggat gatacgggtg cgattataaa
    44941 tatcccgcat ctcgtcaaaa cttaagggca tctgagcaat ctccatcaat acacattagc
    45001 aacaggcgaa aagtcgggcg gcacgaatcc aaaagcactc ttcctcaacg ccgcaccaga
    45061 tgttcaaaga gcgccaccac acgctcctga cggtattccc gggcaatctc cagaccggtc
    45121 tgccctttgg cgttgggagt gtccggtgcc ataccggtac gcaccagcag ctgaataatg
    45181 ccgccgcttc cgaaaacagc tgccgtatgc aaaggcatgt ctccgtagtc atcagtcagc
    45241 tccgggctgg cacccaggct catcagcact tttgcctctt cactgcgccc gagcatggca
    45301 gccacgtgca ggggggtcat gccggtcaca tctgtcgcat ccagggcagc tccccgttct
    45361 acaagcagcg ggatcagatc aatttttttg taggtcaccg ctgtgtgaag cggtgtctta
    45421 cccttgccat cccgggcggt cagatattcc ggatgctctt taagcagttg cagtacggct
    45481 tccgtagatt catgggcaat agcatcatga atgctgacac tgcatccgga aacagccagg
    45541 cacatcacca gaacacaggc gcatagaaag ctttttttca caggaatatt caaaaaattc
    45601 acctgcttgc tgaaggacgg aatgcgaatg tttcttctac gtattatagc ggataaatgc
    45661 cgctttgaac atacttacag cctatcagat tcttccgcta ttttacaaaa acacccctca
    45721 gaaaaaacga cgtagctgcc atatccgccc gattttcagc cagtcgtctc cgctctccga
    45781 tataaattga agcatcggct caggactcgt acaataagca caagcacagt gcagggataa
    45841 tatgaaactc ggcatagaaa tcacaccgct gacacagcag cccacaggca tcgggtatta
    45901 caccctccat ctgctgagtg ctttgtcggc tttacccgac tgccctgaga tatgcggttt
    45961 ttcttcgggc ttgcacaaac cgcaaacggc tgatctcccg atctcctttc gtcggattgc
    46021 cgttcctacc cgcgcgcttt accgctgctg gcgtttgttc aatgcgccca aagcggatgc
    46081 ctttctcggc ggcacggaca tttatcacgc tgtaaattat gtccttccgc cgctgaaaaa
    46141 ggctgccggg gttctgacaa ttcacgatct ggcttttctc gcgcatcccg aatggtgcag
    46201 cccgaaaatt gttgcgcctt tccgtgatgc cgttacccgc catgcccggc gggcagacag
    46261 aattctggtg ccgtcggaag cgacccgaaa ggacgtcctc cggtttcttg acgtcgcccc
    46321 cgacaaaatc agagtgacgc ctctggcagg tgatccttct ttctttccga tgccacgcga
    46381 ggaagcggag gaacaactcc gtgaacacct ccagcttacc gggggctttc ttttatgcgt
    46441 cggaacgctg gaaccccgga aaaaccttcc tacgctgctc cgtgctttct taaaaacaga
    46501 cctgcctcat aaactggtct ttgtgggagg gcacggctgg ggtgatgccc ttcaggcaca
    46561 agtcccttta tcagcacagg acaaaaagcg catcgtcttc acaggctata tcagagaccg
    46621 tggattattc cctgccttat acagcgccgc cactgctttt ctttttccaa gttggtatga
    46681 aggattcggc atcccggcag tggaagccat ggcatgcgga tgccctgtca tcgcttcgga
    46741 cacatcgtct cttcctgaag tggcgggttc tgcagcactc tacgccgcac cgggagatac
    46801 cgatgcctgg gcacgtgcta tggagcacct atgtgctgac ataacattgc aggaagatct
    46861 cagacaaaaa tcgctggcac aggccgaagc cttttcctgg gaaaaatgtg ccctggctac
    46921 tatgcgttgc tataaggaat tattgtaatg cgtatcctcc tcaatgccat gcaaagtgaa
    46981 aaccggagcg gaaccgggcg gtatgtgact gagcttgccc gggcgctgca tgcgcttgca
    47041 ccaaaggagg agctgcagta tctctgcccg gacgggaaaa tccggtgctt tgaggaggct
    47101 tttttgcaaa agcccgtgac accgcttcct ctctgtaaac gtgtgcttac ggagcaatgg
    47161 aaactgccgg ggcttgtccg taaaaaacac ttcgacctcc tgcactatcc tgcaggcacc
    47221 ggtcttctct ttgcatccgc ccctcatgtc ctcactattc atgatttatg ctatttaaaa
    47281 aaccccgact ggttccccaa aagccgggcg cggtactact cctttgtcat ggggaaaaac
    47341 gtgcggcatg cctcccggat ttttgctgat tcacaggcaa cggcccggga tgcggaagcg
    47401 ctgctgtcgg ttccttcaga aaaaatagat gtcattccgc tggggataag cccggacttc
    47461 tcccctgcag ggccggaggc acggaagcag ctgaggcaat gccttttgct tccggagcgc
    47521 ttttttctct ttgtcggcac gcttgagcca cgcaaaaacc tgacaacgct tgtccgtgcc
    47581 tgggcacgac tgccggaata tcttccggag ctggtgcttg cagggcgacc gggctggaaa
    47641 atcacccagg atgctctccg taatgccagc ggcgcacaag cacaccggct tcactttctg
    47701 aattatgtcc cggcagaatt gcttcccgcc ctttacagcg aagcgacggt ttttgtgtat
    47761 cccagtctga tggaaggatt cggactgccg cctctggagg caatggcatg cggaacaccg
    47821 gtcgtaacat ccaacacctc cagcctgccg gaagtaactg gcgatgcggc tgtattgata
    47881 tctcctgaag acgagacgga actagccggt gcgctggagt gggtggcggg agatgaaacg
    47941 ctgtgcagcc ggatgcgtga aaaaggactc agccagtcgg cacgcttcac ctgggaaaaa
    48001 acagcacagc tgactttggc agggtacaga aaaatactgg gcgggtactg aagcagcaat
    48061 ttctgcttcg cttaaatata cttgaacggg ctggtaagaa ttgagggcgg gttctctttg
    48121 taacaggctc caaagtaaaa aaggtcgata agcccttcag ccgcttcttc agaaagatga
    48181 gtatccggca aataagactt cgctgtcaca aaagtgtcca tcatgcctat aagtgccaaa
    48241 gcaagccctt gcgcgtcacc gcccgccagc tcgccttttt ccagcccttc ctgcatgatt
    48301 tcctcaagaa aacgaaaacg gcgcaggcga taaatggtct tatcgaggcg tggcccgcct
    48361 ttgggcggtg cgaagtaaag ctgcagtacc agccgtaccg cctgaggatt ctcctcggca
    48421 aatctgaaag ttgcacgggc aatcgctttc aggcggccta cggcatcact gtaattttcg
    48481 cggatatcag cgagtgttct cgtgtacttt gccagagcag gctccagcaa tttccggaag
    48541 agatcttcct tatttgtgaa ataataataa agaaccggac gggtaacacc tgccccttca
    48601 ataatttctc taatgctggt gccttcatag cctttttctg aaaaaagctg cagggcactt
    48661 tcgaggaggc gtgtttccgc ttcgttagtt tcgcgtattt ctgtaaagtc attatccatt
    48721 ataaggaacc tacctaccgt tcgttcacat gcatagtata cctgatcgca ctgcaaaaga
    48781 ggaaatgttg gtgtgcacta aaacttcgcc gccaatgcgg atttggtaaa tttttccgga
    48841 aatgatatac tgccgatttt gtgtggaaaa ggaatgtttt tgactttttg gggagaattg
    48901 tatgacacaa tttttctgcg gcatagattt cggcaccaca aattccagtg cggcagtatc
    48961 cacggcagaa acagttcatt tgctggatct ggaccctgcc aacgacgctc ccgtatctct
    49021 tccctccctg ctctacatct ccagagaagg cgagaaaatt gtcggacggc aggcggctga
    49081 tctctttatc gcccgcaaca tcgaccgtga aatcgaaaca gcggaggtgt cgctggagtc
    49141 tcttatccct gcattcaccc gggcggagcc ggacaaagac gaatcgtaca acccgctttt
    49201 tacagacccg aatatcgtgg aagctgtaaa cgccaaaatt acggtggaag ttgggtcgcc
    49261 cggtcgtctt tttcagtctc ttaaaagcct gctccgtcag tcttcctttc tttacacgga
    49321 agtgttcggc attaaatatc agatggaagc gctcatctcc tatattctga aagtaatgaa
    49381 agaagctgca gatacccacg ccggttaccc gattgagtcc gctgtatttg gcagaccggt
    49441 tcgcttttcg gagaaggaaa atgaagatca gctggctgaa cgccggctcc gtacagcagc
    49501 atccctggcg ggatttaaaa acgtcgtttt tttctacgag cctgttgccg cctgtgttga
    49561 atatgctgcg gacacccggg acagccagaa gatcatggtg gttgatatcg gcggaggaac
    49621 ctgcgacgtt tccattatgg agtttgccgc tgcagagcat ctcgcggatc ggctctcatc
    49681 aagccgtgtc ttgagcgtat ccggaacgcc cgttgccggt gacgagctgg ataaagcgat
    49741 tattcgcagc cgcctttttc ccctgctggg aagtcgggcg cattacggac cttctaaact
    49801 gccgatgccg caatatattt acagtcaggt gctggattgg caaaatctgt ataagctgaa
    49861 tacggaatct cagattaatt ggctcctcgc tgcagaggtt tcatccaatt atcccgaagg
    49921 gttgaaagcc ctgcgcatgc tgattgagaa caattacggt tacctgcttt ccaaagaagc
    49981 agaagcggca aaaaaacgat tgtccacgga aagcacgacc agcatcacag tggatgcggc
    50041 ccatctccat ctaaaagaca cattggaccg cagtgagttt atcgaaatta ttgaagacac
    50101 cctcgataaa atgtttggct gcattctgga ggcagaagaa aaagcgggac tccggccgga
    50161 cgatctggat tatgtgctta cgaccggagg tacctgcctt atcccggcgg tttatgcgct
    50221 gctggagaaa cgctacggtg cggaaaaact gaaacgacgg cattccttca ccagtgttgc
    50281 ggccggtctt gccacggtgg cacgttatgt tttcagatga tcgttttctc aatcagatcc
    50341 ggctgtaaga tgctcaggca ggaagaaatt tctatttaaa attacttcag gcagcccccc
    50401 gatacacacc atgcttgccg cttcaaagcg gtgatgctgc gtcattgcaa agggcagagc
    50461 acagcagaga ggaatcccgg ctgtacgaat ggcgatggtg cctgattcgc tatcttcaaa
    50521 gcctgccacc ttatgaaagt cctcagggga aattcccatg gtgtgcagcg ccatggaata
    50581 aagatcccga tgcggtttca gacggatctc gctggaatca cttgctgtga tctgcgcatc
    50641 ataaacccgg gcaggcgatt gaaaagcatc aagaagcacg tcttttcttc cggatgaaat
    50701 cttccagtcg cctgcctcgc tgaccagcac gcgaaaaact tcatccagca caatagccgc
    50761 ttcagatcct atggaagaag ttaccaacgc aagttttacg ggatgtcgtt caaaatagcg
    50821 tcccaactgt gccagccctt cccgggacag cactgtcttt gtgtcagctg aatcgttccg
    50881 ttccagaagt gccgcaatca gaggatcacg gagctgcgcc agttcttctc ccagccagcc
    50941 cttgagaagg gcaagagtaa ttccgatgcc gggcattggt tcgatcattg cctttccggc
    51001 cccgccgctg cttcccccct gcttcctgat ttcagcaagt gtggcgtgat aatgccgata
    51061 ataaatttcg atacctgccc gggactgctc cgtcaggctg ttcacagtga gccgaccgag
    51121 acatcgggac accagagccg taagtccctc ctgacgctcc ggttctccgg cagagagcag
    51181 tctgttccag tcgggatcct gatcaagctc cgacaatccc agcacgatca aactgttcaa
    51241 agcctctttt ttccggttaa catccatgcc ctgactgatc gtccacgccg tagactccag
    51301 aaaatgccgg caaatggact ccacctgaaa cgacgcccca tactgttgaa tcagatattc
    51361 cacatgcttg gtcgtactgt ttccgataat attgggataa tcccgcacag gatccagata
    51421 ttgcgacgca ttctccaggc tgcgcccgca catggcagcc gtcatttttg caagcgattc
    51481 gatgcacaat acctcagtcg tggtcgttgt gccatccatg tccatcacca cgccttctgc
    51541 accgtctgcc agttctgtgg cgacgtgagc catgggacac aattcccagg gcggaaaaac
    51601 atagggcggg ttgtgaatca gtgtaaaagg ctggggaatc gcgctcaccc atcgggctgc
    51661 ctgatcggcg gagagatgtt tatcagaaaa ggagtttgtc attagatttt tccggaggct
    51721 gcattgctga aaaacagggt cgggaggtgt gtgcccggca tgacgaaaaa aaacctgccg
    51781 ccgagcgcgc ctattatacc agagtaagcc gttttccctc ctgtttccgc tcataaataa
    51841 ttggttcccg gtacaagcat agtttatact tgataagcgg ccgacacgat gcgctcaaca
    51901 gcaggcaatg acctggagcc ccgatggttt taaagagtct cagacatgaa gcaggtagca
    51961 gtcacaggcg gaaaaatcag cgttgaagaa gtgcctcctc cggttccatc ggaaggtatg
    52021 gctctggtgc aaactgcttt ctctctcatc agttccggca cggaaagcgc ttttctttcg
    52081 accggcggcg ctgccggact ggcactgaaa aaagtactgg atcctgtcac tcgggaaaaa
    52141 ttcaagcaca agcttgccag tacagggatc agaagcacct ggactctggt gcagaataaa
    52201 ctcttcgata ctcaggcgcc cggttacgga tgcgccggga tcatagcgga aaccggcccg
    52261 ggcgtccttc acttgcgaca gggcgaccgg gtagcatgct ccggggtggg ctatgcctgc
    52321 catgccgagc tgaacagcgt accccaccag ctgatcacgc ccgtccccga tggtgtcgat
    52381 ctggatgagg cggcttttgt cagtctgggc gccgttgcct gccaggcggt caggcgtgcc
    52441 gcaccgtcct tcggcgaaat ctttatcgta tccggtctgg gtctggtagg ccagctgaca
    52501 gtccaggttc taaaagcagc gggctgtcag gtcatcggct gcgaccccct tcccttccgg
    52561 cggcagctgg ctgaaagtct cggagctgat ttatcctgtg ctccggaaga tttggcagaa
    52621 gcatcctggc agaactccgg cggcttcgga gtggatggcg tgctggtctg tgccgcatcg
    52681 aaagacagca gtgctgtcaa cgaagcattg cgtgcatgtc gccagaaagg acgggtcatt
    52741 gttgccggtg ctgtcggcat gcatctcgaa agagaagcac tttacctgaa agaactggac
    52801 ttcggactgt cctgttccta cggacccgga cggtacaatc cgctgtatga agagcagggc
    52861 ctggactatc cgatcgggca tgtacgctgg acgcaaggaa ggaacatggc ggcatttctt
    52921 ctcatgatcc gcgacaaacg ggtacaggtg cgcccgctga ttcatctgca ggtctctgtc
    52981 gatgaagcgc ctgccgcgta tgatgccatt cagcaccgga aagaagtgat cgccgccctt
    53041 ctcgagtatc ccgctcccgc gcagcaatca gctcaaacgc cctttaaacg tgtttacgta
    53101 ctgcgcgcca gcccgaataa aaagaaagag cttgcggtgt ccgttttcgg tgccggcgct
    53161 ttcgcttccg gaattctcct gcctgcttta aaaggagttc cgggatgtcg cctccattcc
    53221 ctcgcctgcg caacaggtct gcatgctcaa aaatgggcga aacgtttcca ggccgacgcc
    53281 tgctccacgg actataaaga actgctgaaa gatccggcac tggatgccgt ctttattacg
    53341 acacggcatc acttgcacaa ggcaatggcg ctggatgcca ttgctgcagg caaacatgtt
    53401 tttgttgaaa aaccgctggc actcaccaca gaagcgtgtg aagagatttg tgatgcggcg
    53461 caaaaacagg gagttctgct tgccgtgggg ttcaacagac gctttgcccc gctgtcgtta
    53521 cgggcacaac agcttttttc cggaacaaag caagcgcatc tgctgctcta tcgctgcaat
    53581 gcaggtgctc ttcctcccga tcactgggcg ctggatccgc ttcagggcgg agggcgcatt
    53641 ctcggggagg ctgtccactt ttttgattac gcctgctggt tctttcagga tgttccccgc
    53701 acaattcagg cggaatgcgc ggcgcccgca cagaggggaa aaggcatcga ttccgtttct
    53761 gtgtcccttc ttttttcccg gggcgcactt gccacggtgc tttatacagg cgacggaagc
    53821 agcctgctcg aaaaagaata tatagaaatc catggcactg aaagttctct cactatccac
    53881 gattttaaac agctgactcc atacggtatt ggagccggaa agatcactgc ccggcggatc
    53941 gacaaagggc acacttcact gattacccat ttcatccggg caattcaggg gcatgaagca
    54001 ctctctgttt caggagagga cggcaggctt gccacttata ttgccgaaga aacactacgt
    54061 cagattaatg cttttcataa ggacacctta taatgcgctc ttcgttactt cattttctgc
    54121 tttcgctctc attttctttt ttgctgtgcc tggctgtacc cgtctttgcc gaagtaaaac
    54181 cttcggacga tctttccgag cttgtggaca atatttccat ttcacagggc tggggcaacc
    54241 cgggaattaa cactgccgcc tggatggcgg aaggatcaca acagcccctg cgcattggtg
    54301 acactgtttt tgaaaaagga atcggcacac atgctcaggg agcgattcgt atccctctga
    54361 ataagggata caacactttt tcagcaaaaa ccggagtaca gtggcagggg ggcgggaaag
    54421 gcagtgttgt tttcaaggta caggtagacg gcaaatcagt gtataccggc aaatcgatga
    54481 gcgacagtga tacgcccgct gccctgcatc tttccgtgca gggtgccgat gaattgcttc
    54541 tcctgtccca tgataacggc gacggaatca gttgtgacat ggcaaactgg gtggatgcgc
    54601 agcttatccg tgacacccgt gtcccggtca tcggtgtacc tgtctggaaa ttcggcagta
    54661 aagacgcttc tcctctggcg gaaatggaag gaagttttgc tctggcaggg caaagcggaa
    54721 acgcgcaggc agctttctgc gtccctctgc gttgcgccac cctgtttaca tctcccgggc
    54781 aggacattgc tgtatctttt ccgctcgccg ggatcagcga atcttttacg attgaagcgc
    54841 aactgcaggg cgtcgggaca gccaaaggca ttgcaaccct ttctctggct tcaggcgaaa
    54901 cggtgcgcca acctttgacc gaagatgtga ccacgcttac agtcagaggg aacagcggca
    54961 cagcggagga aagtctgacc cttacgattc agactaactg cagcgccctt gtacgcctga
    55021 aatcgttgac ttacagctgc gacggcatca aaacaccgtt gccttttttc ccgccgcaac
    55081 gcctggctgc cggcagtgac gcaccgcccg ctattgccat gcagcctttg cttgaacagg
    55141 aaatcataga atgggactgg cgcatgcagg acggcatcgg cgccggagca accagaaaca
    55201 gctatgcgga
//
LOCUS       DAOPWB010000005        51303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_611_length_51303_cov_6.419002, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000005 DAOPWB010000000
VERSION     DAOPWB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 51303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51303
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_611_length_51303_cov_6.419002"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PKN92_01170"
     CDS             complement(<1..886)
                     /locus_tag="PKN92_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ47431.1"
                     /translation="MRDKQWNDLLAVLDGKRVSPLPVGFIIDSPWLPAWHGVSVMDYF
                     ADPHIWLEANLNACSTFPDIWFLPGFWSEFGMCSEPAALGARCSFYPAELPYAHPLPD
                     VVKQFPDRPDPKSDGLTPFLLHRLCSMRSKIESHGYTIRFAIARGPLNIASFLMGISE
                     FLMALRLEPDSVHQLLQKINTYLLEWLRLQKNRIDSIDGIFILDDIVGFLGEPDYQEF
                     AHPYLKELFNAFPASVRFFHNDAEGLVCASHLPDLNVNLFNFSHNHSIEQIRALAGNT
                     VTLLGNLPPRDVLAQASAE"
     gene            complement(896..2080)
                     /locus_tag="PKN92_01175"
     CDS             complement(896..2080)
                     /locus_tag="PKN92_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47432.1"
                     /translation="MISKPTVISGIVGAGFSANFHYEGIKRVYSVNPDVRGVFALDQD
                     QAAAFAAKRGITHFSSLDALIDAVDLVHCCVLVSGHEEVAVAALKKGKSVIIEKPLTG
                     YLGDGSSSFHGDTFPKSDALAYGLASVERILEAEKNNKGRIFYAENWVYAPSIQREKE
                     IIEKTKAQILWIHGEEAHSGSHNATYAQWKFSGGGVMIGKGCHPLTAALYLKNVEGRA
                     RTGKPILPATVTARTAALTRMDNFQNDGFIRNDYFDIDDFSMMHVTFEDGTLATVFAS
                     DIILGGIHNWLEVAATNHRTVCNINPNTAMRVYNPRHENFSDIYTVEKIEQKQGWTDM
                     PPDEDWFTGYPQEIEAFYRAAAFGEPVESDSLLGANTISTIYSAYLSAEQGGKEVAIR
                     TF"
     gene            2276..5137
                     /gene="uvrA"
                     /locus_tag="PKN92_01180"
     CDS             2276..5137
                     /gene="uvrA"
                     /locus_tag="PKN92_01180"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNZ47433.1"
                     /translation="MASKNSLNEGSIIIRGAREHNLQNLSLKIPRNKLVVITGLSGSG
                     KSSLAFDTIYAEGQRRYVESLSAYARQFLELQGKPDVDYIEGLSPAISIEQKTTHKNP
                     RSTVGTVTEIYDYLRLLFARAGTPHCYKCGKPIVSQTPQSIVDTVLALPENTRIQIMA
                     PLVRQRKGHYQKIFSEVKQLGFVRVRVDGNFYHVDDDIQLERYVKHDIDVVVDRLSVK
                     EDARKRLTDSVETALKLGKGLIRIWHEPPGGKADEFLQSEHLACIECGVMMEELEPRL
                     FSFNNPHGACPRCTGLGEVQEVIAELVVPNEELSINEGAVHPWSVRRVLDGMYRRALR
                     SLCRHYQISPDTPWNKLPEHFKKIVLYGSGKEEILFSFSNENRNYETRKVFEGVIPNL
                     ERRFKETDSNNAREMIGEYMASRPCPECQGSRLRPEVTSVKVGVHSITSLTALSIQSA
                     MEFFNTLELTPTQMAIAERIIKEIRERLGFLNSVGLNYLTLDRHAGSLSGGESQRIRL
                     ATQIGSGLVGVLYILDEPSIGLHQRDNAKLLATLLRLRNLGNTVIVVEHDEDTIRAAD
                     FVLDLGPGAGIHGGSVVASGTPAQIMKNKKSLTGKYLSGALKIAVPEKRRKGNGAVLK
                     ICKAAHNNLKNITVEFPLGLMIGVSGVSGSGKSSLINETLYPAVARQIYSDFRKSPGK
                     HASVHGLEHIDKIIDIDQSPIGRTPRSNSATYTGLFGPIRELFSKTPESRARGYLPGR
                     FSFNVKGGRCEACEGHGVLTIEMHFLPDVYVPCDTCQGARYNRDTLEIRYKGKNIAEI
                     LDMTVEEGCDFFKNIPPVHSKLITLKNVGLDYIKLGQAATTLSGGEAQRVKLATELAK
                     RHTGKTLYILDEPTTGLHSADVDKLLEVLHQLVDYGNTVIIIEHNLDVLKTADWIIDL
                     GPEGGDGGGKIIGAGTPEDLCKIKASYTGKFLKEKLQRT"
     gene            complement(5138..5902)
                     /locus_tag="PKN92_01185"
     CDS             complement(5138..5902)
                     /locus_tag="PKN92_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47434.1"
                     /translation="MKNRTVFIAGLFCVAFASLNCSQEQPSIQYILPPFGDNIPGNYQ
                     LVAEADFSSESCLNLFEFTDPSSWKPDDRHGRPCLNLIQGVGAYKPPVRSPFSFALLK
                     DLQVEDFILEMDVESTDLAGGNHRDACFIFGFTARDSFYYVHVAAAADPHAHNIFIVD
                     KKPRVNIGASATKGVGWGKEVVHRIRIERTVADGRIAVYFNDMEKPVLEGNDTRLAKG
                     NIGFGSFDNSCCFYEMRLYAPDVTRCEQSLFFTAKN"
     gene            6066..7103
                     /gene="pdxA"
                     /locus_tag="PKN92_01190"
     CDS             6066..7103
                     /gene="pdxA"
                     /locus_tag="PKN92_01190"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNZ47435.1"
                     /translation="MPTSIFTERTKPIIGITMGDSNGIGPEILVKALDTLKQNDSFIP
                     VVYGNAALLNHIAQEYQLSVTFTEKDNIPEIHTDSPDIPVIQCGARAIPYTPGKLDPL
                     AGANAALWIRAATEDASAKRIDGMVTCPVNKDGMLKGGCPYAGHTPMLAALTQTEECY
                     MSLFSSNMRIVHITAHLSMKDAVAAVKKERIYRTIKQCYRTLTQMGFESPRIAVAGLN
                     PHAGEMGAFGTEELEEIQPAVDRARQENIQCTGPLSPDTVFSRMSSGEFDVVVAMYHD
                     QGHIPFKLINMDEGVHATLGLPFVRTSPDHGTAFDIAGTGKARAESMCAALLLSSQWA
                     MHALKQREATA"
     gene            7177..8361
                     /locus_tag="PKN92_01195"
     CDS             7177..8361
                     /locus_tag="PKN92_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNZ47436.1"
                     /translation="MRTFSLLPRSFQVGSHPCAIALADLNGDGIPDIVTADRGELHDA
                     REERPANDELSVLLSDTPFNYIRRHPSLKTGFGPYALALANVDSLKWPDIISANFHTG
                     RNRIISVFLNFKDEGIFKPVEFKAPETSDPYQRHTDGAGEPLYTVPGLTSLAVGDFNN
                     DGFRDLVATGWSNDTIMLMTGHPDHVFNEPVFFPLKGAPRSVCCAHLDDDKNLDLIVS
                     LYATSEIALLQGNGKGEFTEKARFNSRGKLPVSVLYKDINGDGSGDIIVAHADAGDNI
                     SIFYGGRPYQFSISQDIPLGKAQNVLEYGIKDGVAADFNDDGAADLALACQALPGVVV
                     LINESGNTTMPQKFRQEEYLFKEGEPLALATADLDGNGYLDLAVSLGSSNSVSFLKNN
                     KK"
     gene            8398..9885
                     /locus_tag="PKN92_01200"
     CDS             8398..9885
                     /locus_tag="PKN92_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47437.1"
                     /translation="MPQFTTKLFFALLYLGILLFVSGRIVHQMGKFTGYIVLKPGQTE
                     RWVLRESTTEGMLATALRNMPMEIVGLNHFQTAAQFFDRSKHFDAVQLPFYLSLEKEL
                     SREKIKEKYVLTLRRGESSLEKAVRPGQKIDIYDSLIDIVAVQPWSGLVRDERGTPHM
                     VMQVEDTENAAINKLFFENASFFRPGKDFSAEWHWVQSEEEAEHKLQELQHYAGKWGI
                     REAERVHWFETLTPGDGLITSDGTEYTLLGYETSENSSALTVSIEIKDKDTRKILQYP
                     GSDTVGGTSVLLDPGKQKNHIICISWQENSTLCTFFKDGIPEVSRLVQEEEWLDLPVS
                     ENHFLRCTLLQVLEKSFVVTAEESTLKVLCLQVDGTPLALREGESCAAGDLHLSFSRE
                     KILPVPKFLFTTELYNSQSKDSFTLTGSRKIRINNWKLSIAPQFINNGTMPVIIKAER
                     MLGTPSQLVGLTICLLGIIGLTVFRFADWRQEKDRENAFKDDYAG"
     gene            9973..10494
                     /gene="def"
                     /locus_tag="PKN92_01205"
     CDS             9973..10494
                     /gene="def"
                     /locus_tag="PKN92_01205"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNZ47438.1"
                     /translation="MALLKIVVYPNPPLMDVAAPVTDFSERLQQLADDMLETMYHNEG
                     VGLAAPQVGKSIRLIVYCDYVSDPVCLVNPEILERHGCEYSEEGCLSLPSLYATVPRA
                     KSILVRAQDLDGSPFEFEAEDYIARIIQHECDHLEGKVFPDRLDLISKSSVLKEWEEL
                     VRSGKATTKPITD"
     gene            10507..11106
                     /gene="recR"
                     /locus_tag="PKN92_01210"
     CDS             10507..11106
                     /gene="recR"
                     /locus_tag="PKN92_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNZ47439.1"
                     /translation="MFMQSPPVERLLESLRRLPGVGRKSAERYALHLLTLPPEISEEF
                     SAALIAARRAIGWCPLCRNLTDIMPCAICQDPKRDCTTICVVERPTAVLAIEKGGGYR
                     GLYHVLHGVINPLEGIGPEALQIDTLIERVQKNDVKEVIIATNTTAEGEVTALYLAKR
                     MAEFGIQATRIAHGVPMGGGLEYADDVTLLRALEGRLPL"
     gene            11103..11744
                     /locus_tag="PKN92_01215"
     CDS             11103..11744
                     /locus_tag="PKN92_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ47440.1"
                     /translation="MSAKGLFTFHPMRASSIGVLVAQVVWAVLLCFPSAINAQENVAA
                     SRRNAIVTAIEKVAASVVTINVVGYERVQTADPFFDNFMGLFDFAPGMTRTKKKAVEG
                     IGTGFVFDQQGHILTNYHVLQNADHFSSVTLADGRNLDVEVVGTDERTDIAVLKAKTD
                     TPLPYAVLGNSDDLYIGEWVIAIGNPFGSMITDPQPSVSVGVVSTTTEGSKGK"
     gene            11636..12271
                     /locus_tag="PKN92_01220"
     CDS             11636..12271
                     /locus_tag="PKN92_01220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNZ47441.1"
                     /translation="MGHCHWQPLRLYDYRSPTQRQRRGSVYNHRRVKREVGGGDRLYQ
                     DMIQTDAAINPGNSGGPLVNAEGVVVGINTMLFSQSGGYQGIGFAIPGNRARKVAQEI
                     IQFGRRRNPWFGFHGKAVEEMNAYSLRELQIQSRSGVVVTEILKISPAYIAGLQLGDV
                     IEEVNGYPVQYPQDIDFINWDLFCGDSVRLSIDRQGIKKEVRFSVSEIRTE"
     gene            12389..13384
                     /gene="bamD"
                     /locus_tag="PKN92_01225"
     CDS             12389..13384
                     /gene="bamD"
                     /locus_tag="PKN92_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNZ47442.1"
                     /translation="MRKAVFFAACALLLTTEAAFAQWSWTPQTGRFINLKKMPKETAE
                     LQIEYARSFLVAGNYRKALRECDKFVEYYGNDPLAADNLFLRGEVLMAQRQWWNAAKT
                     FQQLLANYPGSDKYDAAIKMQYELGDRFYARGEALAGKKFRFFKKKPYKRAADIYAMV
                     VENQPFAAEAAEAQYKIGLCHFARKQFQDAAFEYKRVIEDYPTTEWVKDASYGLAMCY
                     YKDSYSPAYDQTPSELTVNAIDDFKANFPGDEKNEKLGEMRIEMRNSIAQHYLNSAKF
                     YERRREFPAAKLYYEKIANEFADTQAVDAAKEWLEKNNGVEHVGDKYSEGIRSAL"
     gene            13381..13971
                     /gene="lptE"
                     /locus_tag="PKN92_01230"
     CDS             13381..13971
                     /gene="lptE"
                     /locus_tag="PKN92_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNZ47443.1"
                     /translation="MTQASIFDKPLHWVGLLLAGCAMVISAGCGYTARSSLDPVYQSI
                     SVSPFYDKTNEYGLQIPLTNAVIRKFTADSRLRVKKPEEADLLLEGMIIDFQRKGLIY
                     DRKDETTQALLVVIASIRLTDQKTGEVLWQEPAVSGETTFYTRAAGLTSGRLRGNAET
                     YMNPVRSFTSEEENRAAAEALEQLASEIVYRVLEPW"
     gene            13972..15009
                     /gene="holA"
                     /locus_tag="PKN92_01235"
     CDS             13972..15009
                     /gene="holA"
                     /locus_tag="PKN92_01235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNZ47444.1"
                     /translation="MIINVGDLVSKLDKHKPPSIILLTSGKAPFGKADFEPWLAEAAL
                     HDLLQMYVPEDQKDLALTTLQAGESSPGDVASEVLTLPFLVERKVIVVRHAELFFALS
                     SDKKSPVIPLLECLSDPPDFCLLILLASEANRSRRFYKLCEEKGLVVECPQMEDRVYG
                     QWIKEQLKQKGYSISSGALALMIERVGNSMSDMANAINLLCSFAEQETKLNEQHVLAA
                     CADVAETTVWALTDAIAASDTGKALENLHELLAINKSHEEILGMINWLLESAYKAHPG
                     TKVSLNKPFVEKKVLPLAKKWDVTKMQNALQLCTRTNFQFRNSGADKTLLLETMIIKL
                     AASRGRAAPAR"
     gene            15221..16447
                     /locus_tag="PKN92_01240"
     CDS             15221..16447
                     /locus_tag="PKN92_01240"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNZ47445.1"
                     /translation="MRCSLWLFLFLLAISPLPVPVQAEEGRLFTAELNNAIKTVPMPF
                     LVHENRNYINLETLITELGGLVVNMTDQIEASLNGKTATLITNHREVALENQSFSLSV
                     PCTLLNNALSIAVTDTELFFHNAFGIQIKAEEMKPGADKDLEVMDLDESLLLESVPDL
                     PQPASVSTETASDEGSSEPEKNEQEEDSEESLPSDATAGDFSGMKNIKGCIVIDAAHG
                     GQDAGIVIAEEFKEKEILLQLAGAIKAVLEEKTSLTVVLTREADVALSPGKRVSAAEK
                     AGGAFFVSLESNYSPNIPVSSISIWTDQPPFSDVKKEDAKKENKSELTRQECAEKAAK
                     FGIRLQSNLNKTNTWEKVFVHQAPLLLQRESKIPVILIGTGCLNDPEKLHLLKNPEEI
                     ARISSGIAWALAESLR"
     gene            16587..17144
                     /locus_tag="PKN92_01245"
     CDS             16587..17144
                     /locus_tag="PKN92_01245"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNZ47446.1"
                     /translation="MLTEGRNPFKLAEEDSEEFKQQESSASRDVLTEKKEITLFFTSA
                     AGIGLVPEKCMIESAETTVENCRSALLKMISGPDGQHSLPNIPSDTRVRGVYLKDDGE
                     LILDISSEIIFLQTVPRSIETETMMFFGIVNTMTHEALLGADSKKVTAVRFLFDGMAP
                     QETFPLNFDLSYPLYPAPNWIMTES"
     gene            17147..17974
                     /gene="murI"
                     /locus_tag="PKN92_01250"
     CDS             17147..17974
                     /gene="murI"
                     /locus_tag="PKN92_01250"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNZ47447.1"
                     /translation="MNDLNASSCAPIGIFDSGVGGLTVCRKISELLPFEKIIYLGDTA
                     RVPYGSKAPETVLRYARSCAAVLLKYKIKMLVIACNTATAFALEALQKELDIPVLGVL
                     RPGATAALSRTKNGIIGVIGTRGVINSRSYEKTLKAQRPDIDVYSQACPLFVPFAEEG
                     WVSGDALFHVARHYLEDLKKKKIDTLVLGCTHYPLLKNTIQAVLGEDVILVDSAEETA
                     KDVVESLDKNFLFNKEKEVTQPLFLVTDSPDAFRSTGEKFWGTSLMSVQWVDICSTS"
     gene            18273..19712
                     /locus_tag="PKN92_01255"
     CDS             18273..19712
                     /locus_tag="PKN92_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HNZ47448.1"
                     /translation="MAALGDLLEEEDCLKVNALIRSLLANHETNQQSFPVESRHSSKE
                     SPYPITRHSVFLNSPDALSVLAVTIQSQLGSSCKVSESRLSRKRALIQIYQYDLLCAE
                     VLCIENFEDNKTAPSEDEFEHPAQDGIPEEAPLPKQESESPPKHSVEPEIKEQASELD
                     KPFSEQKRSVARQQEESPLIPEPREIAAKEMQESAPEQEASREFPESDQIIDEEDAET
                     LLEELTILPPEEKVAAEEVHIIEPAKREEHAFPATPDLNAHPCARLAIILDDGGYFKE
                     ETERVLALDYPLTLAILPDTPLGQYIASEGKSRGFETILHMPMQAGNGGKNRFPGELT
                     VSMTGEEIQQRTRECLAMYPGIVGVNNHTGGLFTTQYYAMSSFMDVLAQAGVYFVDSI
                     TTAKSCAFQVAIEKKVPCTKRDIFLDHDNTLSEVRKQFHIAVEQALLNGSCLAIGHFR
                     KNTVFVLEEQLPLVKSKGVRIVPVSELVW"
     gene            19709..20716
                     /gene="tsaD"
                     /locus_tag="PKN92_01260"
     CDS             19709..20716
                     /gene="tsaD"
                     /locus_tag="PKN92_01260"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNZ47449.1"
                     /translation="MRLILGIESSCDETATAIVRDGTEVLSNVVISQIKVHREFGGVV
                     PEIASRMHTEVIYQLAEKAIRDAGLLFTGNLPPVDAVAATFGPGLMGSLLVGLSFGRA
                     LAAAWKIPFVPVHHIEGHLFSAFFGKKKPEYPFLALIVSGGHTQIIACHAPHQYHVLA
                     TTRDDAVGECFDKVARLLGLPYPGGPSIQKAAKEGNPHAYAFPVGMAGKDTLDFSFSG
                     LKTAVLYKMRQEPDADLADVAASFQATAVESLMVKLRLAIEQTGIHRLVIAGGVAANE
                     LLREKAALLDAELFLPPFTYCLDNAAMIAAAAYSRLRTGKALPTDTAAVSTLALQDLP
                     G"
     gene            complement(20705..21781)
                     /locus_tag="PKN92_01265"
     CDS             complement(20705..21781)
                     /locus_tag="PKN92_01265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ47450.1"
                     /translation="MNTCRALRSLGVDVTFACSPKAGSGFNKVRHMAELYKIPVLTFM
                     YLSKHRHPWKNFRDKTALRRHLQEVPYALVHCHLHNDHLIAGAALAESSIPLVRSSYE
                     GVGLSESKNCRALLKRTNLIIEPSEMAKNADLKHFTLPAERFSVIPGAIDLTRFSPSE
                     KLPSWRDKWKLTKDHFIFGIVARMQTHRHYEDLFVAFAHVLRQFPQARLLIVGRGTHQ
                     EKVAYAPVRNLKLEGKVIFTGYLEGDEYTGALGAMDVGIYLTPGSDGTCRTVREFMAM
                     GVPVISSARGMLPELLGQEERGLVTDGSPEGLTEAMVRMITHKALVKRCSEAALAHAQ
                     KHFDLNIYGARILAAYHQLLRLPG"
     gene            complement(21856..25443)
                     /gene="smc"
                     /locus_tag="PKN92_01270"
     CDS             complement(21856..25443)
                     /gene="smc"
                     /locus_tag="PKN92_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNZ47451.1"
                     /translation="MYFKQIYLHGFKSFADPTTVTLDLGTTAIVGPNGCGKSNILDAL
                     RWGLGEQSAKTLRGSHMQDVIFSGSIGRPATGMAEVTLVFDNADGKLPLEYAEIEVTR
                     RVYRNGDSEYLLNKSLCRLKDIQELFMDTGIGTNAYSLIGQGKIDLVLSSKPEDRRYL
                     LEEAAGIIKYKTRKRVAMRKLESAEQNMLRLADIIAEVDRQMRTLKRQVNAAIRHREL
                     TEQVRLLEIRNSWLQYNELSGQITDLKAQLSEATDHYHELAAKTSRQEAELEAITLKR
                     HEASRALSECREKEYSLDSEMERLENQITLYRKEIEFITTRSVEAQAEAERLTLESTN
                     LKNRSGSVSQELLEHTTKIQTLKQALEKSTKELENATKALQDTEIMIEALHERRVEAI
                     NVRNKAHTEIETVNTRITDIHKQRNTVEESIVVQKKRHQEARTVLEQFSEKAKVLQSN
                     LTDIVTKKTESQTLQRNLNQEHARINQEWQSLREKISSEEGRLKSLIELRDSYEGFAV
                     GVRAVMMGKQREFKGLSGIIGPAGDLVSTEKQYRFALEAALGGNINNVVVRLADDAKS
                     AIEFLKENRAGRVTFLPLDTIRPARNEDAASFDNMKGVVGALIQFVECDAEVLPAIQY
                     LLYNTLLVETIDDAIRISRSEKHHPRLVTLEGEVVTSAGTVTGGRTKTDSHGLLGRSA
                     EIEELEESTRQRKKQIKKLEEQGLRIAAKIQDLSDALKSIEASENDIRKTAGAHEVAL
                     ARHATEEANLSAMLDSLEKQKENLNAQEKDLAERLQAARSNIAGMDGDDQAIQGNFEE
                     TQQLALVQKEELAALAKLNTEQRVELAETIKSYEELQRDLDRSSRGGDEMLEKANLHH
                     QQIEAFKKQISDLELKINNTITQSKALSEKRDHAHEAVLVKNKDLQFLQESTDKLTIQ
                     VKENREKENIQQKKVHKLELDCSQKEERVRSFVERIATEYNLVLSSLTEKEVGVDEYD
                     EQEREARIQESRNALQRLGNVNLAAIDEYESLEKREAFLKSQNDDLIKAKETLLGVVK
                     RIDQTTEEMFLQTFKQISENFQHYFRRLFNGGQARLFLLDDNTPLDCGIEIEARPPGK
                     KPQTISLLSGGEQALTAIALLFSIFAAKPSPFCVLDEVDAPLDDVNVGRFLNIVNEFT
                     EHSQFIMITHNKQTMAHANAIFGVTQQEAGVSQLVSVHLEEAEKVAGVS"
     gene            complement(25544..26335)
                     /locus_tag="PKN92_01275"
     CDS             complement(25544..26335)
                     /locus_tag="PKN92_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNZ47452.1"
                     /translation="MLVLFIGDIVGKPGREAVRVVLPDLIKTLCIDVVMANAENIAHG
                     TGATPPLIKKLEAEGVHLFSLGNHAWRHPEMIKGIDALPSVARPANFSTHAPGKGRAV
                     YTLPDGRHCTLINLIGRVFMEPANCPFDAIERELDSRTGDSPFIFVDVHAEATSEKAA
                     LAWHLDGRCSAVLGTHTHVQTADERILPGGTAFISDVGMCGPYNSILGVKSELVLKKS
                     ITGLPVKWEIADGPVQFNAVLVETDDRTGRAISVKRIQQILQLDS"
     gene            complement(26498..27946)
                     /locus_tag="PKN92_01280"
     CDS             complement(26498..27946)
                     /locus_tag="PKN92_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47453.1"
                     /translation="MLDFIKKYQTALMAGVLLFSLGAGVLLNNQLRHLRESEAFYRWL
                     LAAAVNERLFQENEDPSEDALFFARCTALAEKFPSFSPSDKVDGLSMIGSIAKNYEND
                     KRLWFFAESDLAKEERAEFLQLAKKQKLQFATDIEYAEAQAGGVNVFNLFFGFRKVAA
                     NLVWLEVDRYWHQGNVYRMQTLMRTCVALDPQFVEAYLVGAWHLSYNATAQMQDTPWP
                     QREWNAKYGQCLGEKERFYYYGVDFLREGIRRNPRNYKLYFDLGFGIYKQKLEDYPNA
                     VKYLSEAIRLPHDRWVPRQLFLCQELNGQYGDALEGWKDYVERYPGSMTAVEVAPRFI
                     MRNEGMLYEKEARTLRKKAKESSDPAEAEELRAQAENYWKKARDTWNALDDPYGEGRK
                     LMMDALDLREEGRYLEAIATLDKARWDTGALFEEFSSLIMEIKLEAHIPLSVSEKKAL
                     LRESEVGLCSGMPDEERQRRLKSAESIESTFQ"
     gene            complement(27946..28776)
                     /locus_tag="PKN92_01285"
     CDS             complement(27946..28776)
                     /locus_tag="PKN92_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ47454.1"
                     /translation="MTFLSSVFGGPWCIAVYTYREGIRRKLLIGFLILSLFVIFGSSF
                     ISSFLDPSAETDIDLKLIKDICVTAIAIFGALITVYISASVVPAEMETKVIYTVLSKP
                     VRRVQYLLGKFQGIQLIILVNLLLMGGLFFIALYMRQGIMPTLLLWSIFLTYFEFLII
                     SAFTFAVSCAATSPLVPTLGGLFIYITANLTEYLKDVEHRAGQTGQFFDNLIGKIASA
                     LYSILPNLQNFSIKMQIVNGQPNDPPTDVLIPWLVLYSLVYALAGFSCAYLVFRRKEV
                     "
     gene            complement(28773..29723)
                     /locus_tag="PKN92_01290"
     CDS             complement(28773..29723)
                     /locus_tag="PKN92_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ47455.1"
                     /translation="MTTVIRTTALNKIYEGAFRGQDVHALKDLDLDIRQGEIFGYLGP
                     NGSGKTTTIKMLLGLLFPTSGKIEILGSESIDSATVKRNIGYLPEGAYYPDFLRGEEI
                     LAYYGKLYGLRGKELKKRVDETIELVGMQHARKRLLRGYSKGMRQRIGLAQALISDPK
                     ILILDEPTTGLDPIARKEIRDLLLQLREQGITLLISSHELLEVEMISDRVAILFEGVL
                     QKIGSMAELLTQRELSMTVDHLSESSVSDLTDKGIQVEDRTGNRAVLRLPFHMSSYDG
                     VEMCKAHSLNLLSISPRRETLEELFVRIVSTASKAREVVQ"
     gene            complement(29904..30848)
                     /locus_tag="PKN92_01295"
     CDS             complement(29904..30848)
                     /locus_tag="PKN92_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47456.1"
                     /translation="MKIKQQTIARFLEFMVGFYFIAGAIPKAWDIDKFSVQMAAYKIL
                     EAPHFLLTASFFTVFLEFSLGAALLLALRCKGWLYALVQAMILAFSGMILYSWIFHGL
                     KDCGCFPVIAMSPPVSLFKNLIIFASILFSWKTLSPVSLFSFAASKKSAVKFVLSCFA
                     GIAGLVIAWQQGDFTALTRSNDSAAGIYAQFQIFSNDGYFDLSDGTYLVPVMSVTCPE
                     CKSKVPELNALWEANDLPPMIALVYEDSPGDLDEFLALVSPLFPTYSLGDRALLYFTL
                     IDDDPFKFVIVRNGRELASWNGEVPEHAEIERFLEDNG"
     gene            complement(30893..33763)
                     /locus_tag="PKN92_01300"
     CDS             complement(30893..33763)
                     /locus_tag="PKN92_01300"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ47457.1"
                     /translation="MNATFAENTDTARWTASWIWNKRDNYACYNDTIEARKEVTLPAI
                     KAATLRITADTSYRLLINGEWVADGPSRSWPEHYQYDVIPVAGFLKEGENTIHIIAKF
                     YGIGTFHQIPVQAGLLAQLDVTASDGSNFSVGTDERWQVRDAVAWIQNTPKQSVQMGP
                     FEIYDARQPAKEFVPATVLCKALEGPWKNFESRDCPFLSRIVQSPAAVHAVTVVEKLP
                     VKSFIFPTTNWLYGDIVYSNNHVAVTGAYATVFEVDTACEVLMNPDANQVYLDGKNAN
                     GKAFHVEPGRHLLYFVLSEPWGHWRHDSELIIHCEEAFRLSHPLGDAANGSWCFIYPD
                     PKVKYRFADYAWALLSVEEQARIEKEVSDCITGFKDAAPDLDSFPRFVQDKKMRTISD
                     AEITPSPHYLFENRKVLSSGNDRVVNADALLSSSSGTIIKPSEKGDIEIILDFGTQNV
                     GYYSFELHAEEGLILDLFGVEYIAPDGAVQHTERYRNGMRYICSEGDNTFLSLNRRSQ
                     RYLFLTFRNQTREAVLKNFKLIESTYPVKEEGHFACSDEGLNRIWQISAYTLKLCMED
                     VFTDCPLYEQTLWVGDSRNEALFGYTAFGSADIAKRCIKLAAHSLDHHPMVLAQVPTT
                     WETIIPVWSFLWNIMIRDYYEFTKDKDFMEWAFPYAMKNLRNAAEYADEKGLFSAPFW
                     NMFDWSGIDDRHNTVLHNSMFMVGAIDATAKIAQVLGQEEDLAWLKDRRSILVNALNA
                     LWQEDAGAYPDSIHADGKISPKYSQHTAFLSLLFDIAEDRMRPSLLAHLMDPPENMTR
                     IGSPFAILYLFSALEKEGLGHEVIQRIRSAYQPMLDIEATTVWETFAGALNYKGAFPT
                     RSHTHAWSSAPVHFLNRIILGIVSDNTGCASFTISPDITGLTWASGSTACYNKEPVSV
                     SWTVKESVLEIKASGPDGVDLHFKPNDSHGSLEIVFNGTKVR"
     gene            complement(33909..34439)
                     /locus_tag="PKN92_01305"
     CDS             complement(33909..34439)
                     /locus_tag="PKN92_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ47458.1"
                     /translation="MRELSLHLLDVIENSMRAKATIIRIAVIKKEGFLTLSVEDDGPG
                     LPVTPEQALDPFFTTKKGKKTGLGLSFFKAACEQAGGDFALGTSELGGVSVIARFQYD
                     HWDRAPLGNFMDTLQTVTLAAPDIVWVCHIESETVSSTLVLQNFLEAAQDNLLSGLRN
                     FRQAVEAALLAADVQS"
     gene            complement(34432..35115)
                     /locus_tag="PKN92_01310"
     CDS             complement(34432..35115)
                     /locus_tag="PKN92_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HNZ47459.1"
                     /translation="MTPEAIVYKAITSDLEVIAICDHNSAANIQAVQEAAFSIAGDLF
                     LVLPGMEITTSEEVHLVALFPDAEAALAASDEVGKTLPCAANNPEQCKEQPILNAAGE
                     VIGHADRFLSFASAFSLDDAALLVRRYGGLCVAAHVDRPSFSVVSQLGFIPPAPVFDA
                     LEISASGYKQGKALDFSARKETLISSSDAHYPDNIGDGRCFLEMKNLSFQEVKLALAG
                     AIGRRCSLA"
     gene            complement(35175..35528)
                     /locus_tag="PKN92_01315"
     CDS             complement(35175..35528)
                     /locus_tag="PKN92_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HNZ47460.1"
                     /translation="MLLTDLVQELGLTPLTPQLPLDEAAGIERAYVSDLLSDVMGNAP
                     GDGVLITVQVHANVVAVASLAAQKAIIFAMNRRPDENTCLKAAEEGIHLFSSSLPAFE
                     IAGMLYRNGVRGLKE"
     gene            complement(35532..37121)
                     /locus_tag="PKN92_01320"
     CDS             complement(35532..37121)
                     /locus_tag="PKN92_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNZ47461.1"
                     /translation="MKYEIEAVIEKENYSNAGRTSRKIKELLIQIGVDSAIIRRAMVA
                     LYEAEMNVAIHTNGGKVRAAIASDVLDVTIEDEGLGIADLQQAMTAGFSTAPDSAREM
                     GFGAGMGLPNIQRNTDRFSMSSTLGKGTVMRFAISLAPHSSDGAVGTALSMEPSKCIH
                     CLKCVHVCPTQALRVHRKGPEILTHLCVDCTACMRVCPTGVFDFKADHALPEFEEGQI
                     YCLPEALRGQFPLLSSPDALRGLLSPLTVENTIFSHTATWWMNGYLEFYRKRFVNNEF
                     KIAPLCPSISRLIQLRFPSLLPHMLPLMTPLLSLREKIIDSDLVIVPSCPAEFSQAKG
                     IHALNAVHAVHPRFFHSLLEKRINKEAVSQHPQTDSFQPAKDCLVVTGINQVCHFLDQ
                     IENGYLQDAGLVVLYACVGACYGSPVWTAPGAIAEKRSRYYDVMEGAKKELNPLYRVQ
                     PLMPRVGARLDEDMKRAIQKLNKIDEIAKTFPGNNCGACGSPSCLTHAEDIVLARTTD
                     YECPFLVLSQSNASENASTEK"
     gene            complement(37118..37369)
                     /locus_tag="PKN92_01325"
     CDS             complement(37118..37369)
                     /locus_tag="PKN92_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47462.1"
                     /translation="MSDLLGAVSEQMLIVTHLSNQGMIRLIELMDASAICLLNGTPPC
                     PELQEAAENAGTPLIVSPFGMYESCGIIYSLFKEDTSSL"
     gene            complement(37496..37894)
                     /locus_tag="PKN92_01330"
     CDS             complement(37496..37894)
                     /locus_tag="PKN92_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ47463.1"
                     /translation="MYVVVIDDDDDVLEWCRIILEADGFTVDCFNDPSIAFNAIKVNR
                     PDILVSDLMMNNLDSGFELAAKVKSLPGYEELPIILMSAASTRHGYDFIPRNDDDLRA
                     MHIDAFMAKPAEANKLLSKIRELVVRSTKK"
     gene            complement(37979..40126)
                     /locus_tag="PKN92_01335"
     CDS             complement(37979..40126)
                     /locus_tag="PKN92_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47464.1"
                     /translation="MYGSPADCVSCKQKLFIPEEHEIGEDTRIIYLRDHLELLRKPGV
                     YVRDNIPEKKVLSSDLQESSTSAGNSKISFLKESATEASKKDESSEFDLFFQDTLIGS
                     KSNASVYSSESNIPLDSFEPLQKWAGYSKALNRARHSQSPEQEEYFEQLELDLENALL
                     NIKNYLAEEIEDSKKKIAEIAQEIADFALSLSNGDVALAYYQQQVRELRLQREFLARK
                     LYNLECWFEYSPDAILFPPAEVTLEDFDRRQFKGGFEFSQWPHPSTPLSSYYCSLLKD
                     SFENKDRLSQQIKTEKQLLASTESVATEAASLLSRTHCQIHFCRSRLEKIIEDCDLDL
                     RSFIKCQITLLDEIPRNKAEWRKTKSLTSALQAETRNLIARQEELEVFFKASSPEDLK
                     TGVTAAPAPDANETTFFPIVQIFALSLATCSFLCFFFILHLVDPDLPVLPALLPSFLL
                     FSHLLLLWRSVWVRSLPFVLLFWLLEMAASAYSLFYLQGKITVVPTDTAVLNNFALYL
                     FPAGAALINGGNMSAFIAMVHRKIREKMAVQFITFLVVTVAVFIAFISVKDVNALFSP
                     AFTTQDKTLSQNLVTNTPPNPSEKIPLPRTYSQEIITDAETEDADDVSEEDDMSSAEG
                     EATAPLRDNEMRFHLNGVVHVPGKDPRFRGVLQHPDGTEESVSLLLGEQVVGNWVASE
                     YDPAGKKLIVSDGKTLLVLQAGKSVSLPSEEEE"
     gene            complement(40350..40715)
                     /locus_tag="PKN92_01340"
     CDS             complement(40350..40715)
                     /locus_tag="PKN92_01340"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNZ47465.1"
                     /translation="MLKEIVTWLIGLGLVVLVGYGYVQQREMSSRYKELQQTELEIER
                     MKEEIQSLEEKVSAARLKVQSLVSDPTEIEATIRQERQLTRPGEQVFRIEEAPAPVST
                     DSNTDSPQKTEAVDSEPAL"
     gene            complement(40771..42051)
                     /gene="eno"
                     /locus_tag="PKN92_01345"
     CDS             complement(40771..42051)
                     /gene="eno"
                     /locus_tag="PKN92_01345"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNZ47466.1"
                     /translation="MTRIMSVQGREILDSRGNPTVEVEVLLQSGFSGRAAVPSGASTG
                     ENEAVELRDGDKARYLGKGVLKAVNNVNEELGPELIGMDALGQREIDATLIAFDGTKT
                     KGKMGANALLGISLAVARAAAAALELPLYQYIGGVNAHTLPVPMMNILNGGQHADNNV
                     DVQEFMVMPAGAPSFREALRMGTEIFHALKSVLKDAGLNTAVGDEGGFAPSLESNVAA
                     IEVILKAIQKAGYKAGEDVFIALDPASSEFYENGKYVLGAENASLSSEEMVAYWADWV
                     KKYPIISIEDGLHENDWAGWKALTDTLGKKTQLVGDDLFVTNVDYLSRGIKEGVANSI
                     LVKVNQIGTLTETLDACQMAHRAGYTTVMSHRSGETEDSTIADIAVAINAGQIKTGSA
                     SRSDRTAKYNQLLRIEEQLGEQAYFPGLAAFKHK"
     gene            complement(42173..43174)
                     /locus_tag="PKN92_01350"
     CDS             complement(42173..43174)
                     /locus_tag="PKN92_01350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ47467.1"
                     /translation="MKNFKSLISFCAIAIYLISLSSTGTAAAATFVDGVVASVGRDPV
                     LYSDVMQEMFSELGDRSSLEMSEEDMVQHLNPLYKEALEREIELRILFKEATALGVVV
                     PDDKIEDRISDLKKQFPNAEAFRTALNATGLTPSTLRERIRRQMMAYTVRMSKQQQFE
                     KEAIISESDLAQYFQDHKSEFKFETRYLVRRFFLKAPFDAKKRDEVRQRLNDIRIRIK
                     DGADFTALAKEMSDGPEASEGGSLGWVLPGDLVEPLNSALLSLPANELSDILETDFGF
                     HFLRIEEIQTEGTTPYEEARIDIEPLLRRKQAEEQYRRWMNLLRQRSEIRILRDAIQ"
     gene            complement(43199..47320)
                     /gene="rpoC"
                     /locus_tag="PKN92_01355"
     CDS             complement(43199..47320)
                     /gene="rpoC"
                     /locus_tag="PKN92_01355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNZ47468.1"
                     /translation="MAKYVPTTGEHFDAIQIRLASPQEILKWSRGEVKKAETVNYRTH
                     KPEKDGLFCERIFGPAKDWECGCGKYKKVKHRGITCDRCGVEITESKVRRERMGCIKL
                     ASPVTHIWFLKNNPSSLGNLLDLSIKQLERIIYFEQYIVVDPGDTLLPKMKLLTDEEY
                     KEEREKYGPDRFTALMGAEAIKALLTDLDLNALSSELQVQFHTATSQQAKKKANKRLK
                     IVEALRKSGNDPAWMVLDVIPVLPPDLRPLVLLDGGLYATSDLNDMYRRVVNRNVRLK
                     RMLEMRAPEVVLRNEKRMLQEAVDALLDNGRHGRPVTGKNGNRQLKSLSDTVKGKQGR
                     FRQNLLGKRVDYSGRSVIVVGPELKLHECGLPKRMALELFEPFIIQQIMSRGISQTIK
                     TAKAFITRGRDEVWDILETVIKDHPVLLNRAPTLHRLGIQAFQPVLIEGNAIRLHPLV
                     CRAFNADFDGDQMAIHVPLFPPAQLEAHLLMMSSSNIFSPSNGLPIVTPSQDIVLGIA
                     WMTKVREGAKGEWQSNWTGKKGEILAFGQIYSSPIEVVQAYDAGVVDIHAAIKVHFNG
                     KVIDTTVGRVLFAEALGSEVTIEDVNRQHDQTSIGNVIAKVYQRHGHKRTVALLDRLK
                     ETGFKYATLSGLSIAIDDMVVPEKKYKLLREAEESVQKINEMYEGGLISTKEKRNKIV
                     DQWTTTNNEVGETMVKALEENEQGFTGLHLMYQSKARGSKSQIRQLAGMRGLLAKPSG
                     EIIETPIMSNFREGLSVVEYFISSHGARKGLADTALKTANAGYLTRRLVDVAQDVTIE
                     MEDCGTMAGIYREALIEEAEVISPLSERIVGRYALEDIYLSGESEPLVQANEEITEAA
                     AKRIEEEQLHGVKIRSVLTCNAPKGVCALCYGRNLATGKKVELGEAVGIIAAQSIGEP
                     GTQLTMRTFHIGGAASKTVESESQRCKTAGTIQFSNVQTARNSEGFTVVIKRGGEIQI
                     LTEDGKTAERIPVTPGCRMQIEDGAKVKSSQILFTRDPYNDLIVAEKSGRIELQGMLE
                     GVTTRLELNAETGREELIVTEHKHDVHPQINIVDEKGDVLAFVTVGSGTQVQVRTNDQ
                     VCAGDLLGKTSRQFSKTKDITGGLPRVEELFEARNPQQPAIISHIEGTVELSTTAAGN
                     RKLKVIPPVGKEREYSIPPAKHVNYHTGDRISAGDRITDGSVILDDMLEVLGEDEVRT
                     YLLNEVQQVYRLQGVRTDDKHIEIIIRQMLRKVRIQADPGDTPFLAHEEVDKNLHSRI
                     NAEIGMRGGRPAEAAAIIQGISKAALTTNSFIAAASFQKTSMVLTEAAIHGKSDLLEG
                     LKENVIIGHLIPAGTGSRHYQNSTAKFPKEDFDEYFDIESDVSTETADNEENN"
     gene            complement(47324..51172)
                     /gene="rpoB"
                     /locus_tag="PKN92_01360"
     CDS             complement(47324..51172)
                     /gene="rpoB"
                     /locus_tag="PKN92_01360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNZ47469.1"
                     /translation="MAVAPPTERTFLGKIHDDYQLPDLIEIQTKPFSDFLQWETLPEE
                     RKCQGLQAVFSEVFPIVSPNGSMRLEFVSYSLTPPKYSVQECLDRALTYSASLKAVLR
                     LSSFEDIGNTNEKQEKLVSEQEVFLGELPVMTKTGTFIINGAERVIVSQLHKSPGVSF
                     EKKTHTNGRELLFARIIPNRGEWLEIEYDVSDTLWVIVNRKGKLLATTFLKCFGIVDH
                     DEILQIFYTMETVRFERGHPKIDKKKVPLESLENRFLGAEVIDLETGEILADAGEELT
                     QAKLNRIVASSVKELKLIAEKDLINDKAIVNTLQQDATTTEDEALHAFYSHSRPEDPT
                     TTAQCRSHFNKTFHDPSRYDLASVGRYKINRKLGLQIDPDTKTLTAEDVVKTMQYLSR
                     LRSGKGYLDDIDHLGNRRVRSIGELLYNEMRKGLTRMERTARDRMNYNEEENLNPQGL
                     VNPKPINSAIKEFFGRSQLSHFMDQINPLAELTHKRRLSALGPGGLTRERAGFEVRDV
                     HPTHYGRICPIETPEGPNIGLMASLATYAHINEYGYLETPYRRVENGRVQHDQIERLS
                     AFDEENYTIAQANAPLDAKGNFTEELVLCRLQDDFPRVAPAKVDYMDISPKQMVSVAA
                     ALIPFLEHDDANRALMGSNMQRQAVPLLCSESPIVGTGLEHITACNSGTVVRAEREGV
                     VEYADSEVIRIRVKEKKGDNPFHSIEEVHKLKKYSRSNQSTCINQRPIVQKNERVVPG
                     QIIADGPAVDHGELALGKNVLVGFMSWEGYNFEDAIILSEELLEKDAFTSIHIEMHEM
                     QARDTKLGPEEITRDIPNVGEEILRNLDEDGIVRIGAKVGPGDILVGKVTPKGETELA
                     PEEKLLRAIFGEKAEDVRDASLYVKPGTQGVIVDVKICSRREKGSDTQAKRAMKKEET
                     QIVQEFNQRIAEIKSTFEEMVSKKMVGLQILADVYDMVSGDLIFEKGKKIRPKYLSSY
                     DYSYLAQLKVQDKKIQKEFVKLVNLAAMEEAKLVAVRDAQIERIRKGDELPAGVNKTV
                     KVYVATKRRMSVGDKMAGRHGNKGVVAKILPAADMPFLPDGTPLQILLNPLGVPSRMN
                     VGQLLETQLGWALGALGMKAATPVFNGVSEGTIREYLKKADLPENGKIRLRDGRTGNY
                     FHHETTVGQIYMMKLNHLVDDKIHARAIGPYSLVTQQPLGGKAQSGGQRFGEMEVWAL
                     QAYGAAYTLQELLTIKSDDIQGRTKAYEAIVKGRHEIEPSTPESFNVLVREMQALCLD
                     IVKLVKSESLSDQDDYTMED"
BASE COUNT        13534 a        12169 c        12051 g        13549 t
ORIGIN      
        1 tttcagctga tgcctgcgcc agtacatctc ggggcggcaa atttcctaga agcgtcactg
       61 tattcccggc aagcgctctt atttgttcta tggaatggtt gtgtgaaaaa ttaaacagat
      121 taacgttaag atccggcaaa tgcgaagcgc acaccagccc ttcagcatca ttgtgaaaaa
      181 agcggacaga agcaggaaaa gcgttaaaaa gctcttttaa atacgggtgt gcgaactctt
      241 gataatcagg ctcccccaga aagccgacta tatcatctaa aataaagata ccgtcaatgg
      301 aatcaattct atttttttgc aaccggagcc attctagaag gtaagtatta attttttgca
      361 gcagctgatg gacactgtcc ggctccaaac gcagtgccat taaaaactcg gaaattccca
      421 tcagaaaaga ggcaatattc agtggacccc gcgcgatggc gaaccggata gtgtatccat
      481 gcgactcaat cttgctgcgc atggagcaga gacgatgcag taaaaatggg gtgagcccgt
      541 cgcttttggg atcaggacgg tcaggaaatt gtttcacaac atccggcagc ggatgggcat
      601 agggcagctc tgcaggataa aaactgcagc gggctcccag agcggcaggt tcggaacaca
      661 tgccgaattc tgaccagaac ccaggcaaaa accaaatatc aggaaaagta gaacaggcat
      721 ttaaatttgc ttcaagccag atatggggat cggcgaaata atccatcacc gatacgccat
      781 gccaggcggg cagccacggg ctgtctataa tgaaaccaac cggaagcggg gacacgcgtt
      841 ttccgtccag tacagcaagc agatcattcc attgtttatc tcgcatggtg ttcctctaaa
      901 aagtgcgtat agccacttct ttaccgccct gttccgctga aagatacgct gaatagatcg
      961 ttgaaatggt gtttgcgccc aaaagactgt cgctttcaac aggctcccca aaggcagctg
     1021 cccgataaaa agcctcaatt tcctgaggat acccggtaaa ccagtcttca tcgggcggca
     1081 tatctgtcca cccctgtttt tgctcaattt tttcaactgt ataaatatcg gagaaatttt
     1141 cgtgacgcgg attataaaca cgcatggctg tattggggtt aatattgcag acagtccggt
     1201 ggttggtcgc agcgacttca agccagttat ggatgccgcc taaaataata tcggatgcaa
     1261 aaacagtagc caaagtgccg tcttcaaatg ttacgtgcat catagaaaaa tcatcaatat
     1321 caaaatagtc atttcggata aagccgtcat tctgaaaatt atccattctg gtcagagcgg
     1381 cggtccgggc tgtaacggta gcgggcagaa tcggttttcc agtacgggcg cgcccttcca
     1441 catttttcag atagagtgcg gcagtaaggg gatggcatcc tttgccaatc ataacgccgc
     1501 caccggaaaa tttccactgg gcataagtag cgttatgcga tccggaatgt gcctcttctc
     1561 catgaatcca aaggatttgg gcctttgttt tttcaatgat ttccttttcc cgctgaatgg
     1621 agggagcata gacccagttt tcagcataga atattctgcc tttgttgttt ttttctgcct
     1681 caagaatacg ctcaacactt gccagaccgt atgccaaagc atcgcttttt ggaaaagtat
     1741 ctccatgaaa agaactgctt ccatcgccca gatagccggt caagggcttt tctatgatta
     1801 cagatttgcc tttcttaaga gcggctacag cgacttcttc atgaccggag accaacacgc
     1861 agcagtggac aagatcaact gcgtctataa gggcatccag ggaagaaaaa tgggtaatac
     1921 cacgtttagc agcaaaagct gcagcctgat cctgatccaa agcaaagacg ccacggacat
     1981 ccgggttcac gctatataca cgtttaattc cttcataatg aaaatttgct gaaaaaccgg
     2041 cacccacaat tccggaaatg accgtcggtt tagaaatcat ggtttctttt cctgtgttta
     2101 ctgtagaaat tgacgctaag aaattcttta catattatag ccaccattga ttgaaagaag
     2161 aaattagaag taaaatactg catgataatg agattcggtc ccgattctct tttggtgttt
     2221 tacgctctat cctgtcactt tttttctctg cttcttatta aggacttgcc tctttatggc
     2281 ttctaaaaat tcgctcaatg aaggctccat tattattcgc ggagcccgtg agcataattt
     2341 acagaacctt tcactaaaaa taccccggaa caaactggta gtgattaccg ggttgagcgg
     2401 ttcaggaaaa tccagcctcg ctttcgatac tatctatgcc gaaggtcagc gtcgctatgt
     2461 tgaaagtctt tctgcttacg cccgtcaatt tttggaactg caggggaagc cggatgtcga
     2521 ctacatcgaa ggtcttagcc cggcgatctc catagagcag aaaaccacgc ataaaaaccc
     2581 tcgttctact gtaggaacag tcacagagat ttacgactat cttcgtttac tcttcgcacg
     2641 ggctggaacg cctcactgtt ataaatgcgg gaagcccatt gtttcgcaga caccccagag
     2701 catagtggat actgtccttg cactcccgga aaatacccgt attcagatta tggcaccgct
     2761 ggttcgccaa agaaaaggac actaccagaa aatcttttca gaggttaaac agcttggctt
     2821 tgttcgtgtt cgcgtagatg gcaattttta tcatgttgat gatgacatcc agcttgagcg
     2881 ctatgtcaaa catgatattg acgttgtcgt ggaccggctg agcgtcaagg aagatgcacg
     2941 gaaaagactg accgattctg tcgaaactgc gctgaaatta ggaaaaggcc tcatccggat
     3001 ctggcatgaa ccgccgggcg gaaaggcgga cgagtttctg caaagtgagc accttgcctg
     3061 tattgaatgc ggcgtgatga tggaagaact ggaaccgcgt ctcttttcct ttaataatcc
     3121 tcatggcgcc tgcccgcgct gtaccgggct gggcgaagtt caggaagtca ttgctgaatt
     3181 agttgttccg aacgaagaac tttctatcaa tgaaggtgcg gtgcacccct ggagtgtccg
     3241 gcgtgttctg gatggaatgt accgacgcgc tttgcgttct ctgtgccgcc attatcagat
     3301 ttctccagat acaccgtgga ataaactacc ggagcatttc aaaaaaatag tgctatacgg
     3361 ttccggcaag gaagaaattc ttttcagttt ttccaatgaa aaccgaaatt atgagacccg
     3421 caaagtattt gaaggggtta ttccgaatct ggaaagacgc tttaaggaaa cagactccaa
     3481 taatgccaga gaaatgatcg gagaatatat ggcgtccagg ccatgtccgg aatgccaggg
     3541 aagccgcctg cgacctgaag tgacttctgt aaaggtcggc gtacattcca ttacttctct
     3601 cactgcttta tctatccaaa gtgccatgga gttttttaac accctagaat taacgccaac
     3661 acaaatggca attgcggaac gcattattaa agaaatcagg gagcgccttg gctttctgaa
     3721 cagcgtaggg ctgaactatc tcacgctgga caggcatgcc gggtcgcttt ccggcggtga
     3781 gtctcagcgt atccggctgg cgacacaaat cggaagcggt cttgtcggtg tgctgtacat
     3841 actcgatgag cccagtatag gattgcatca gagagacaat gcgaaactgc tggcgacact
     3901 ccttcgactc agaaatttgg gtaatacagt gatcgttgtt gaacacgatg aagataccat
     3961 ccgtgcggcg gactttgtgc ttgatctggg gcccggtgca ggaattcacg ggggcagtgt
     4021 ggtggcgtcg ggcacgcctg cccaaattat gaaaaacaag aagtccctta caggcaagta
     4081 cctttccggt gctttaaaaa tagctgttcc tgaaaaaaga cggaaaggta acggagccgt
     4141 tcttaagatt tgcaaagcgg cacacaataa tctgaaaaac ataaccgtag agtttcctct
     4201 gggcctcatg atcggcgtat cgggcgtgtc cggctcgggt aaatcaagcc tgattaatga
     4261 gacactctat cctgcagtcg cccggcaaat ttattcagat tttcgcaaaa gtcccggaaa
     4321 acacgcatct gtgcacggtc ttgagcacat tgataaaatc atagatattg atcagtctcc
     4381 catcgggcgc acacccagat ctaactcggc gacctacacc gggcttttcg gacctatccg
     4441 ggaactcttc agcaaaacgc ctgaatcccg ggctagagga tatctgcccg gacgtttcag
     4501 ctttaatgtg aaagggggac gatgtgaggc atgcgaaggc catggcgttc tgacaataga
     4561 aatgcatttc ctccccgatg tttacgtgcc ctgcgatact tgccagggtg cccgatacaa
     4621 cagagacacg cttgaaataa ggtacaaagg gaaaaatatt gccgaaattc tggacatgac
     4681 tgttgaggaa ggctgcgact tttttaaaaa cataccccct gttcatagca agcttataac
     4741 attgaaaaat gtgggacttg attacataaa actgggtcag gcagcgacaa ctttatctgg
     4801 cggtgaagcg cagcgggtca aactggcaac ggaactggca aaacgacaca cagggaaaac
     4861 gctttatatt ctcgatgagc cgaccacagg acttcattct gctgatgtag acaaactgct
     4921 tgaggtactt catcagttgg tcgattatgg aaatacagtt attattattg agcataatct
     4981 ggatgttctg aagacagcag actggatcat tgatctgggt ccggaaggag gagacggagg
     5041 cggcaagatc ataggcgccg gtacccctga agatctctgc aaaatcaaag cttcctatac
     5101 gggtaagttt ctgaaagaaa agctgcagcg cacgtaatca gtttttggca gtgaaaaaaa
     5161 ggctttgctc acaccgggtg acatcgggtg catacagccg catttcataa aaacagcagc
     5221 tgttgtcaaa tgaaccgaaa cctatattcc cttttgccag ccgggtgtca tttccttcca
     5281 gaacaggctt ctccatatca ttaaaataaa cagctattct gccgtctgcc acggttctct
     5341 caatgcggat tctatgaaca acctcttttc cccagccaac gccttttgta gcggaagcgc
     5401 caatattgac gcgcggtttt ttatcgacaa taaaaatatt gtgggcatga ggatcagcag
     5461 cggcggcaac atgaacgtag taaaaggagt cccgggctgt aaacccgaaa ataaaacagg
     5521 catcccgatg gttgccgccc gccaggtctg ttgactcaac atccatttct agaataaagt
     5581 cttccacctg cagatctttc agcagagcaa aagaaaatgg agagcggaca ggaggcttat
     5641 aagcaccgac tccttgaatt aaattaaggc aagggcggcc gtggcgatca tctggcttcc
     5701 aggaagaggg atcggtaaat tcaaataaat ttaaacaaga ttccgatgaa aaatccgctt
     5761 ccgccaccag ttgatagttg cccggaatat tgtcgccgaa cggcggaagg atatactgga
     5821 tagatggctg ttcctgtgaa cagttgaggg aagcaaaggc aacacaaaaa aggccggcaa
     5881 taaaaacagt tctatttttc atagagagac ttcccgaaag tgagcttcaa acaattgctg
     5941 aacgttacta tacgacgaaa gcagccgcat aatcgagtcc tttttctgat ggtttcaatg
     6001 cgaggcagtt tttacgcctc tgcagataat ggtacactaa cagaagatta attaaggaaa
     6061 aagaagtgcc tacctcaatt tttactgaaa gaacaaaacc tattattggc attaccatgg
     6121 gtgacagcaa tgggataggg ccggaaatat tagttaaagc gttagatacg ctaaaacaga
     6181 atgactcttt tatccctgtg gtgtatggaa atgcggccct attaaatcat attgcccagg
     6241 aatatcaatt gtcggttaca tttacagaga aggacaatat tccggaaata cacaccgatt
     6301 ccccagatat ccctgtgatt cagtgcggag cacgggctat cccttataca ccaggtaagc
     6361 ttgacccgct tgcgggtgcc aatgctgctc tctggattcg tgcggcgact gaggacgctt
     6421 cagcaaaaag aatagatggc atggtgacct gtcctgtaaa taaagacgga atgctcaaag
     6481 gagggtgccc gtatgccgga cacacgccga tgctggcagc gcttactcaa acagaagagt
     6541 gttatatgag tcttttttca agcaacatgc gcattgttca tattaccgcc cacctttcca
     6601 tgaaggatgc agtggcagca gtaaaaaaag agcggattta cagaactatc aagcagtgct
     6661 atcgcacctt aacccaaatg ggctttgaat cgccccgcat cgctgtagca ggattaaacc
     6721 cgcatgccgg cgaaatgggc gctttcggaa cagaagaact tgaggaaatt cagcctgctg
     6781 tcgaccgcgc ccgtcaggaa aatatacagt gcacaggtcc tttgtccccg gacaccgttt
     6841 tttcccggat gagcagcgga gaatttgatg tcgtcgtggc gatgtaccac gatcagggac
     6901 atattccctt caaactgata aacatggatg aaggtgtgca tgcaactctc ggactccctt
     6961 ttgtgcggac ttcacctgat catggaacag cctttgacat tgcaggtaca ggaaaagccc
     7021 gggcagaaag tatgtgtgcc gctttgcttc tgtcttcaca gtgggcaatg catgctttaa
     7081 aacagaggga ggcaacagca taacatgcgt ttctctgtta catgggcatt tgccagtttt
     7141 cttttactct gcgctggtgc atccggggaa agtggagtgc gcaccttttc attgttgcct
     7201 cgcagttttc aggtaggttc ccatccctgt gcaattgctc tggctgattt aaatggcgac
     7261 ggcatcccgg atattgtgac agcggatagg ggagagctgc atgatgcccg ggaggaaaga
     7321 ccggctaatg atgagctttc ggtgcttctg tccgatacac cctttaacta cataagacgc
     7381 cacccgtctt taaagaccgg attcggtccc tatgcactgg ctctggcgaa tgtcgattca
     7441 cttaaatggc cggatatcat ttctgccaat tttcatacgg ggcgcaacag gattatttct
     7501 gtctttctta acttcaaaga cgaaggcatc tttaagccgg ttgagtttaa agcacctgaa
     7561 accagcgatc cttatcaacg gcataccgat ggtgccgggg aacctttgta cactgtgccg
     7621 ggattgactt ctctggcggt tggtgatttt aacaatgatg gttttcgtga tctggtggca
     7681 acagggtgga gcaacgatac catcatgctc atgaccggac acccggatca tgtatttaat
     7741 gagcctgttt tcttcccctt aaagggagcg ccgcgttctg tatgctgtgc gcatcttgac
     7801 gacgataaaa accttgattt gattgtttcg ctctatgcca cttcagaaat tgctcttttg
     7861 cagggaaatg gcaaagggga atttacagaa aaagcgaggt tcaactccag aggaaaactc
     7921 cctgtctctg tactgtataa agatattaat ggtgacggca gtggcgatat tattgtcgca
     7981 cacgcagatg ccggtgataa catttcaatt ttttacggag gccgtcccta tcaattttca
     8041 atttcgcagg atattcctct aggcaaagca cagaatgttt tggaatacgg catcaaggac
     8101 ggtgtagctg ctgattttaa tgatgacggg gctgctgatc ttgcgctggc ttgccaggct
     8161 ttgcccggcg ttgtagtgct gataaacgaa tcaggcaaca caacaatgcc tcagaaattt
     8221 cgacaggagg aatatctatt taaagaaggc gaacctctgg cactggctac ggcagatctt
     8281 gacggaaacg ggtatctgga tcttgctgtc tctctgggct cttctaattc agtaagtttt
     8341 ctaaaaaaca acaaaaagtg attgtttcaa gcctattaac gataaagcga agtgtaaatg
     8401 ccacagttca cgacaaaact attcttcgca ctgctctacc ttggaattct tctttttgtc
     8461 tccggtagaa ttgtccatca aatggggaaa tttaccgggt atattgtttt aaaacccgga
     8521 cagactgagc gttgggtctt gcgtgagtca acaacagaag gaatgctggc aactgccttg
     8581 cgcaatatgc cgatggaaat tgtgggactt aatcattttc aaacagctgc gcagttcttt
     8641 gaccgttcaa agcatttcga cgctgtgcag ctgccttttt atctttcatt ggaaaaagag
     8701 ctgtcccgtg aaaaaatcaa agaaaagtac gttctgactc ttcgacgggg cgaatcctct
     8761 ttggaaaaag cagtacgtcc cggtcagaaa atagatattt atgattcgct gattgatatt
     8821 gtggcggtgc agccctggag cggactcgtt cgtgacgaac gcgggactcc acacatggtt
     8881 atgcaggtcg aagatacaga aaatgcagcg ataaacaaat tgttttttga aaatgcttct
     8941 tttttcagac cggggaaaga tttttctgcg gaatggcatt gggtgcaaag tgaggaggaa
     9001 gcagagcata aactgcagga actgcagcat tatgccggaa agtggggcat tagagaagcg
     9061 gaaagagtgc actggtttga aacactcacc cctggggatg ggttaattac ttccgacgga
     9121 actgaataca cccttttggg atatgaaact tctgaaaact cttctgcttt aactgtttca
     9181 atagaaataa aagacaaaga tacgcgtaaa attctgcaat acccgggcag cgataccgtc
     9241 ggcggaactt ctgttctctt agatccgggc aaacagaaaa accacattat ttgtatttcc
     9301 tggcaggaga acagcactct ttgcactttt tttaaggacg gtattcctga agtctcccga
     9361 ctggtacaag aggaagagtg gctggatctg cctgtttccg aaaatcattt tctgcggtgc
     9421 actttgttgc aggtactgga aaagtccttt gttgttactg cagaagaatc tactcttaaa
     9481 gtgttatgcc ttcaggtgga cggcacgcct ttagcgctgc gagagggcga aagctgcgct
     9541 gccggtgatc tgcacctttc attttcacga gaaaaaatcc tgcctgtgcc aaaatttctt
     9601 tttactactg aactttataa ttcccaatca aaggattcgt ttacacttac cggcagcagg
     9661 aaaatccgca tcaacaactg gaaactttct attgcgccgc aatttattaa taatggaacc
     9721 atgccggtga ttatcaaagc ggagcgtatg ctgggcacgc cgtcgcaact ggtaggattg
     9781 acgatttgtt tgctgggcat tatcggactt accgtttttc gttttgctga ctggagacag
     9841 gaaaaagaca gagagaatgc atttaaagac gattatgccg ggtaatttgt tggataaccc
     9901 ggtggaatgt ttacggattg gcacatgttt ttcgttaaat aactgattat gagctttgaa
     9961 ttggaggcca ctatggcttt actaaaaatt gttgtttatc ctaaccctcc gcttatggat
    10021 gtggctgctc ctgtaacaga tttctcagaa cgattacagc aattggctga tgacatgctg
    10081 gagaccatgt accataatga aggtgttggg ctggctgccc cgcaggtggg aaaaagtatt
    10141 cgcctgattg tctactgcga ttatgtatcc gatccggtat gcctggtgaa tccagaaatc
    10201 ctagagagac atggctgtga atacagtgaa gaaggctgct taagccttcc ttcgttatat
    10261 gcaacggtgc ctcgtgcaaa atccattctt gttcgtgctc aggatctgga cggaagtccc
    10321 tttgagtttg aagcggagga ttatattgcc cgaatcatac agcatgaatg tgaccatttg
    10381 gaaggtaagg tttttcctga tcgtttggat ctgatctcca aaagttccgt gctaaaagaa
    10441 tgggaagaac tggtgcgatc cgggaaagcc actaccaagc ctatcactga ctaaaaggga
    10501 ttttccatgt ttatgcagtc accgcctgtt gagcggcttt tggaatcact gagacggctg
    10561 cctggtgtag gacgcaaatc ggcagagcgc tatgccctgc atctgctgac attgccgcct
    10621 gaaatctcag aagaattctc tgccgccctt atcgcagcgc ggcgcgctat tgggtggtgt
    10681 cctctctgca gaaaccttac cgatatcatg ccttgcgcta tttgccagga tcccaaacgg
    10741 gattgcacaa ctatatgtgt ggttgaaaga cctacagctg ttctggcaat tgaaaagggc
    10801 gggggatacc gtggacttta ccatgttctg cacggtgtca ttaatccttt ggaaggaata
    10861 ggtccggagg cattgcaaat tgatacgttg attgaacgtg ttcagaaaaa cgatgtaaaa
    10921 gaggtcatta ttgcaaccaa taccactgct gaaggcgaag tcaccgcgct ctatctggca
    10981 aagcgcatgg cggagttcgg gattcaagca accagaattg ctcatggcgt gcctatgggc
    11041 ggtggccttg aatatgcgga tgacgttacg ttacttcgtg ctttggaagg gcgattgccg
    11101 ttatgagcgc taaaggtctt tttacttttc accccatgcg cgcctcttca attggtgtac
    11161 ttgtcgcaca agtcgtgtgg gccgtgcttt tgtgttttcc atctgcaatc aatgctcagg
    11221 aaaacgttgc cgcttccaga agaaatgcaa tcgtaacagc catagagaaa gtcgctgctt
    11281 cagttgtaac cataaatgtt gtcgggtatg aaagagtgca gacagcagat cctttttttg
    11341 ataacttcat ggggcttttt gactttgcac cgggaatgac acgcacaaag aaaaaagcag
    11401 tagaaggaat aggtacagga tttgtttttg atcagcaggg acatattctc accaactacc
    11461 atgtattgca gaatgccgat cacttttcgt ctgtcacgct tgcagacgga cgaaatcttg
    11521 atgttgaagt ggtagggaca gatgaacgca cagacatcgc tgttcttaaa gccaaaacag
    11581 atacgcccct tccttatgcc gttctgggaa actccgatga tctctatatt ggagagtggg
    11641 tcattgccat tggcaacccc ttcggctcta tgattacaga tccccaaccc agcgtcagcg
    11701 tcggggtagt gtctacaacc acagaagggt caaaagggaa gtagggggcg gtgaccgttt
    11761 atatcaggat atgattcaaa cagatgcggc catcaacccg ggcaatagtg gcgggccgct
    11821 ggttaatgcg gaaggggttg tggtgggaat taacaccatg cttttttctc aaagcggcgg
    11881 atatcagggg ataggcttcg ctattcccgg caatcgggcc agaaaagttg cgcaggagat
    11941 tatccagttc gggcgccgaa gaaacccctg gttcggcttt catggaaaag ctgtcgaaga
    12001 aatgaacgcc tacagcctgc gggaacttca gattcaaagc cgttcaggcg ttgtagtgac
    12061 ggaaatcctt aagatttctc ccgcttatat cgccggtttg cagctgggtg atgtcataga
    12121 ggaagtgaac gggtatcctg ttcaatatcc tcaggacatt gattttatca attgggacct
    12181 cttttgtgga gactctgttc gcctttctat tgacaggcag gggattaaga aggaggttcg
    12241 cttttcggtc agtgaaatcc gcaccgaata acggcggcaa taggcaggtc tttttgtatt
    12301 tactgccttc taactgctac tatgttaccg ttcagataca ggtgtctgaa ccgattggat
    12361 gagggctgat taccttagga gaacttccat gcgtaaggct gttttcttcg cagcatgtgc
    12421 actgttactg actacagaag ctgcttttgc acaatggtcc tggacaccgc aaacagggcg
    12481 ttttatcaat ctgaaaaaaa tgccgaaaga aacggctgaa cttcagattg aatatgcaag
    12541 aagttttctc gttgccggca attacagaaa ggcattgaga gagtgcgata aatttgttga
    12601 atattacggc aatgatcctc tggcagcaga caaccttttt ctgcgcggtg aagtgctgat
    12661 ggcgcaaagg caatggtgga acgccgccaa gaccttccag cagctcctcg ccaattatcc
    12721 gggttcagac aaatatgatg ccgccataaa aatgcaatat gagctgggcg accgttttta
    12781 tgcacgtggt gaagcgcttg caggaaaaaa attcagattc ttcaaaaaga aaccctacaa
    12841 acgggctgct gatatctatg ccatggtagt ggagaaccag ccttttgccg ctgaagccgc
    12901 cgaagcacaa tataagatcg gtttatgcca ctttgcacga aaacaatttc aggatgccgc
    12961 tttcgagtat aagcgggtca ttgaagatta tcccaccaca gaatgggtta aggacgcatc
    13021 ttacgggctt gccatgtgct attacaaaga ctcttacagc ccggcctatg accagacacc
    13081 cagcgaactt accgtgaatg ctattgatga ttttaaggca aatttccctg gcgacgagaa
    13141 aaacgaaaaa ctcggggaga tgcgcataga aatgcgcaat tctatcgctc aacactatct
    13201 gaatagcgca aagttttacg aacggcgcag agaattccct gctgctaaac tgtactacga
    13261 aaaaatcgcc aatgaatttg ctgatacaca agcagttgac gctgccaaag agtggctcga
    13321 aaaaaacaat ggtgttgaac atgtaggtga taaatattcc gaaggtatca gatccgcttt
    13381 atgacgcagg cttctatatt cgataagcct ctgcactggg tagggctttt gctggcagga
    13441 tgcgcaatgg tcatcagtgc cggttgcggt tacacggctc ggagctcttt agacccggtt
    13501 tatcagtcga tcagcgtctc gcccttttat gacaaaacga atgagtacgg gctgcagatt
    13561 cccttgacga atgcagtcat tcgaaagttt accgctgatt ccagacttcg cgtaaaaaaa
    13621 cctgaagaag ctgatcttct tttagaagga atgatcattg acttccagcg caaaggtctg
    13681 atctatgatc gcaaggatga gacaacccag gcgttgcttg ttgttatcgc ctctatccgg
    13741 ctgacagatc aaaagactgg cgaagtactg tggcaggaac ccgcagtttc cggagaaacc
    13801 actttctaca caagagcagc aggcttgacc agcgggcgac ttcgcggtaa tgcggagacc
    13861 tatatgaacc ctgtacgttc tttcacttca gaagaagaaa accgtgctgc tgccgaggcg
    13921 ctggaacagt tggcatcaga aatcgtttat cgggtgctgg agccctggta aatgattatc
    13981 aacgttggcg atctcgtaag taaattagac aagcacaagc ctccctccat tattctgctc
    14041 acttccggca aagccccttt tggcaaggct gattttgaac cgtggcttgc ggaagcagcg
    14101 cttcatgatt tattgcagat gtatgtgccc gaagatcaaa aagatctggc gctcactacg
    14161 cttcaggcag gggagtcttc ccctggcgat gtcgcctctg aagtcttgac gctccctttt
    14221 ctcgtagaaa gaaaagtgat tgttgtgcgt cacgcagaac ttttttttgc actttcttcc
    14281 gataaaaaaa gccctgttat tcctttattg gaatgcctga gcgatccgcc tgatttctgc
    14341 ctcttaattc tcttagcctc cgaggccaac aggagccggc gcttctataa actctgcgaa
    14401 gaaaaagggc ttgtggttga atgtcctcaa atggaagacc gtgtatacgg gcaatggatt
    14461 aaggagcagc tgaagcaaaa aggctattcc atcagcagcg gcgcccttgc tcttatgatt
    14521 gagcgggtgg ggaatagcat gagtgatatg gcaaatgcca ttaatctcct ttgttccttc
    14581 gcagaacagg aaaccaaact caacgagcag catgtgctgg cggcctgtgc cgatgttgcc
    14641 gaaaccactg tgtgggcact taccgacgcc attgcggctt ctgatactgg aaaagctctc
    14701 gaaaatcttc atgaattgct ggcaattaat aaatctcatg aagagattct cggaatgatc
    14761 aattggcttc tggaaagtgc ctataaagcc catcccggca caaaggtcag tttaaacaag
    14821 ccctttgtgg aaaagaaagt gctgcccttg gcaaaaaaat gggatgtgac taaaatgcag
    14881 aatgctctgc aactctgcac cagaactaat tttcaattca gaaacagcgg tgccgacaaa
    14941 acgctgctcc tggaaaccat gatcattaaa cttgctgctt ccagaggacg tgcagcgcca
    15001 gcccgatgaa gagagctttc cttttgccgg ggcattcaga atttctgtga aaactaacta
    15061 aaaattagtc tttttttctc tgtaatttga ttgaaatcag gcaatccggt agaattcctg
    15121 ttgtatgcgc taatgtcgtt ttctttttca gaatgtaccg tgccaaagca gtttgcaggt
    15181 tcttttttct cttgtcttcc tcaaaggaaa ttaagtttat atgcgttgct ctctctggct
    15241 gtttttattt cttctcgcaa ttagtcctct gccggtgcct gtccaggcag aagaagggcg
    15301 cctttttaca gctgaactga acaacgcaat aaaaaccgtt cccatgccct ttctggtgca
    15361 tgaaaacaga aattacatta atctggaaac ccttatcaca gaattgggcg ggctggtcgt
    15421 aaatatgact gaccagatag aggcgagcct gaacggaaag acagcgacgc taatcacaaa
    15481 ccaccgtgag gtagctttag aaaatcaaag tttttcttta tctgttccct gtacattatt
    15541 aaataacgca ctcagcattg ctgtaaccga taccgaactt ttttttcaca acgcttttgg
    15601 aatacagatt aaagcggaag agatgaaacc cggggccgat aaagaccttg aagtaatgga
    15661 tctcgacgag tcactccttt tggaatcagt acccgatctt ccgcaacctg cttctgtttc
    15721 cacggaaact gcgtcagatg aaggctcaag tgaacctgaa aagaacgagc aggaagaaga
    15781 ctccgaagaa agcctgccct ctgatgcaac agcaggcgat ttttcaggta tgaaaaatat
    15841 taaaggttgc atagtgatcg atgcagccca cggcggacag gatgccggta ttgttattgc
    15901 agaggaattt aaagaaaaag agattctttt gcagctcgct ggcgcaatca aagctgttct
    15961 tgaagaaaaa acgtccttaa ctgtagttct caccagagaa gcagatgttg ctctctctcc
    16021 cggcaaacgg gtctcggcag ctgaaaaagc cggaggtgct tttttcgtgt cgctggagag
    16081 taattattct ccaaacatcc ctgtatcctc tatttcaatt tggacagatc agccgccctt
    16141 tagtgacgtg aaaaaagaag atgcaaaaaa agaaaataaa agtgaactta cccggcaaga
    16201 atgcgctgaa aaggcggcaa aatttggcat caggcttcaa agcaacttaa ataaaacaaa
    16261 cacttgggaa aaagtttttg tgcatcaggc gcctttgctt ttacaaaggg aaagcaaaat
    16321 accggttatt ttaatcggga caggatgttt gaatgatccg gaaaaattgc acctgttgaa
    16381 gaatcctgag gaaatcgcgc gtatatcttc tggaatcgcc tgggcgcttg ccgaaagcct
    16441 ccggtaaaag aaccgtgcct taatcttata aatcggaata cacaatgcag ttttctacca
    16501 gtcacatctt taaaaagctt ggcttgattc tgtgggcatt tgccacgctg attctatgct
    16561 ttcttgttct gctgctgtcc agacatatgc ttactgaggg gcgcaatccc tttaaactgg
    16621 cagaagaaga ctcggaagaa tttaaacagc aggagtcatc tgcttcacgc gatgtcctga
    16681 ccgaaaaaaa ggaaatcacc ctttttttca cctcggcggc cggcatcggt cttgtcccgg
    16741 aaaaatgtat gattgagagt gccgaaacaa ccgttgaaaa ttgcaggtca gctttgctca
    16801 agatgatttc aggtcctgac ggccagcact ctttacctaa catcccttcc gacacccgtg
    16861 ttcgcggggt ctacctgaaa gatgatggtg aactcatttt agatatttct tccgaaatta
    16921 tttttctgca aacagttcct agaagcatcg aaacggagac tatgatgttt ttcgggattg
    16981 tgaacacgat gactcatgaa gcattgctcg gggctgattc aaaaaaagtg acagctgtcc
    17041 gttttctttt tgacggcatg gctccgcagg agacttttcc tctgaatttt gatttatctt
    17101 acccgctcta cccggcgcct aactggataa tgacagaatc ctgataatga acgatctgaa
    17161 tgctagcagc tgcgcaccta tcggtatttt tgactctgga gtaggcggat taactgtttg
    17221 ccggaaaatt tccgagctgc tgccttttga aaaaatcatt tatcttggtg atacggcacg
    17281 ggtgccttac ggatctaaag cccctgagac cgtacttcgc tatgcccgtt cctgtgcggc
    17341 ggtgctccta aaatataaaa taaaaatgct ggtcattgcc tgcaacacag caaccgcttt
    17401 cgctttggag gcgcttcaaa aagaattgga tatcccagtt ctgggggttc tcagacctgg
    17461 agcaacagct gctttgagtc gaactaaaaa cgggataatc ggtgttattg gtaccagagg
    17521 cgtaattaac agccgttcct acgaaaagac tttaaaggca caacgccccg acatagacgt
    17581 ttacagtcag gcttgtcctc ttttcgtgcc ttttgctgaa gaaggctggg tttcggggga
    17641 tgctcttttt catgtcgccc gacactacct ggaggacctg aaaaagaaaa aaattgacac
    17701 cctggtcctg gggtgtactc attatccgct tctgaaaaac accatacagg ctgttttggg
    17761 cgaggatgta atacttgtcg acagtgcgga agaaacagcg aaagatgttg tggagtcgct
    17821 ggataaaaat tttctgttta ataaagaaaa agaagtgaca caacctctgt ttctggtgac
    17881 cgattctcct gatgccttcc ggtctactgg cgaaaagttt tggggaacca gcttaatgtc
    17941 ggtacaatgg gttgatatct gttctacctc ttaattgaaa caggaaattt ttaatggcga
    18001 aacagaagca aaaaaagaaa agtcctaact tttctccttt gtttcgcgct gttatgcata
    18061 cttatttttc cgctgcagtt ctttcctttc tatccgtttt tatttttgcc ctctttatta
    18121 gcctgaaaga cctcgttccg gacaaatggt ctgccccctt tcagaaaatc agccacagca
    18181 ataatgcttt tccagaacaa ttgccagagg atcctgtacc ggcaacaata atccgtaact
    18241 acgaattttc tcccgagccg caggagttta caatggcagc tctgggtgac ttgctggagg
    18301 aagaggactg tctaaaagta aatgccttaa tccggtccct gcttgcaaat catgaaacca
    18361 atcagcagtc ctttccggtg gaatcgcgcc acagctccaa agagtctcct tatccgatta
    18421 cacgccactc tgtattcctt aattctcctg atgcattgag cgtacttgcc gtaacaatcc
    18481 agagccaact tggcagttcc tgcaaagtgt cagaaagccg tctttctcga aaacgtgcgc
    18541 ttatccaaat ttaccagtat gacctcttgt gtgccgaagt gctgtgcatc gaaaacttcg
    18601 aagacaacaa aactgctcct tctgaagacg aatttgaaca ccctgcgcag gatggcatac
    18661 cagaagaggc tccacttcca aagcaggaaa gcgagtcgcc tcccaaacat agtgtcgagc
    18721 cggagatcaa agaacaggcc agtgaattgg ataaaccgtt ttctgagcag aagaggtctg
    18781 ttgcgcggca gcaggaagag agccctctca tccccgaacc aagagaaata gcggcaaagg
    18841 agatgcagga aagcgctccg gagcaagagg cgtcccggga attcccggaa tcagatcaaa
    18901 tcatagacga agaagacgcg gagacacttc tggaagaact tactattctg cctcccgaag
    18961 aaaaagtggc tgctgaagag gtgcacataa tagagcccgc caaaagagaa gagcacgctt
    19021 ttccggcgac gcctgattta aatgcacatc cctgcgccag gctcgccatt attttggacg
    19081 acgggggcta ctttaaagaa gaaacagaga gggtgctggc tttagactat ccacttacgc
    19141 tcgcaattct tcctgacacg cctcttggcc agtacattgc ctccgaaggg aagtcccgtg
    19201 gcttcgaaac gattcttcat atgcctatgc aggcaggaaa tggtggaaag aaccgtttcc
    19261 ccggtgaact aaccgtttca atgactggag aggagattca gcagcgaacc cgtgaatgtc
    19321 tggctatgta tccgggtatt gttggcgtaa ataaccacac aggcgggctt tttacaacac
    19381 aatactacgc aatgtcttct tttatggatg tactggcgca ggcgggagtc tatttcgttg
    19441 acagcatcac gacagctaaa agctgtgctt ttcaggtggc gatagaaaag aaagtgccct
    19501 gcaccaaacg tgacatattt ctggatcatg ataatacttt gtccgaggtg cgtaagcaat
    19561 ttcacatcgc tgttgaacag gctctcctga atggaagctg tctggcaatt gggcacttca
    19621 gaaagaatac tgtttttgtt ttagaggaac agcttcctct ggttaaaagt aaaggggtgc
    19681 ggattgtgcc cgtttcggag ttggtctggt gagattaatt ctgggaatag aaagctcatg
    19741 tgatgaaact gcgacagcca tagtacgcga tggcaccgaa gttttatcta atgttgttat
    19801 atcgcaaata aaagtgcatc gggaatttgg aggtgttgtg cctgagattg cttcccgaat
    19861 gcatacagag gtgatttatc agttggcaga aaaggctatt cgtgatgccg ggctgctatt
    19921 cactgggaat cttcctcctg tggatgccgt cgcggcaact ttcgggccgg gtctcatggg
    19981 gtcccttctc gtaggattaa gtttcgggag ggcattggct gccgcctgga agattccttt
    20041 tgtgcccgta caccatatag aaggacacct cttcagtgct tttttcggta aaaaaaagcc
    20101 ggaatatcct tttcttgctt tgatcgtcag cggcggacat acccaaatca ttgcctgtca
    20161 tgctcctcat cagtaccatg tccttgccac aacccgtgac gacgccgtag gggaatgctt
    20221 tgacaaggtg gcccgtctcc tcggattgcc ctatccgggc gggccttcca tacaaaaagc
    20281 tgcgaaagaa ggaaatccgc acgcctatgc ttttcctgtc ggcatggcag gaaaagacac
    20341 gttggatttc agtttcagcg gattgaagac cgcagtcctt tataaaatgc ggcaggaacc
    20401 ggatgccgat ctcgcagacg tagcagcttc ttttcaagca acagcggtcg agagcttaat
    20461 ggttaagttg cgcctggcaa tagagcagac aggcatacat cgtcttgtta tcgctggcgg
    20521 cgtggctgca aacgagttgt tgcgtgaaaa ggcagcattg ctggacgcgg aattatttct
    20581 tccgcccttt acctattgtc ttgataatgc cgcaatgata gcggcagcag cttattcccg
    20641 gctgagaaca ggtaaagcgc tgccaacgga cacagcagct gtttcgacgc ttgcattgca
    20701 ggatttaccc gggtaaacgc aaaagctgat gataagctgc cagaattctg gctccataaa
    20761 tattcaaatc aaaatgcttc tgtgcgtgcg caagcgccgc ttcgctgcag cgtttcacta
    20821 gagccttatg tgtaatcatg cgcaccattg cttcggtcaa gccctcagga gaaccgtccg
    20881 tcacaagtcc gcgctcttcc tgcccaagca attcaggaag cataccgcgg gccgacgaaa
    20941 taacaggcac tcccattgcc ataaactcac gtactgttcg acaggttcca tcactgccgg
    21001 gagtcaggta aatgccgaca tccatggcac ccagagcccc cgtgtattca tcgccttcga
    21061 gataacctgt aaaaatgacc tttccttcca attttaaatt tcggacagga gcataagcca
    21121 ctttttcctg atgggtacct cttcccacga tcaaaagccg tgcttgcggg aactgacgta
    21181 gcacatgagc aaaagcgaca aagaggtctt cataatggcg gtgtgtctgc atgcgtgcaa
    21241 cgataccgaa gataaaatgg tcttttgtaa gtttccattt atcgcgccag gaaggaagct
    21301 tctcggacgg agaaaagcgg gtgaggtcaa tagcgccggg tatcactgaa aatcgctctg
    21361 caggcagggt aaagtgtttt aaatctgcat tcttcgccat ctcagacggc tcaataataa
    21421 gatttgttcg cttcagaaga gcacggcagt tcttgctttc tgacaagcca accccctcat
    21481 agctgcttcg caccagggga atcgacgatt ctgccagtgc ggctcctgcg attaaatgat
    21541 cattatgaag atggcagtgg acgagagcgt agggaacttc ttgcaaatgg cgacgcagcg
    21601 ctgttttatc ccgaaagttt ttccaggggt gcctatgctt tgaaaggtac ataaaagtaa
    21661 gcacaggaat cttgtagagc tcagccatgt gcctgacttt attaaagccg gaccctgctt
    21721 taggagagca tgcaaaagtg acatcgactc ccagggatct taaggctctg caggtgttca
    21781 caaccggctc agcaggtcct gtccacttgc agttgctaaa tagatgcagt actttgagag
    21841 taggggaaag cacagttatg atacgcctgc aactttttcc gcttcttcca ggtggacact
    21901 gaccaactgt gaaacacctg cctcctgctg agtaacgcca aaaatagcat tggcatgcgc
    21961 catggtttgt ttattatgcg tgatcataat gaactggcta tgctccgtaa attcattgac
    22021 aatattaaga aaacgcccta cattcacgtc atccagcggg gcatccacct catccagcac
    22081 gcaaaagggg cttggcttgg ctgcaaagat actgaagagc aatgctatgg ctgttaaagc
    22141 ctgctctccg ccggacaaca gggaaatggt ctgaggtttt ttccctggag ggcgcgcttc
    22201 aatttcaatt ccgcaatcaa gaggcgtatt gtcatcgaga agaaataaac gcgcttgtcc
    22261 gccattaaaa aggcgtctga aataatgctg gaagttctcg gaaatctgct tgaaggtctg
    22321 aaggaacatc tcttcggtag tctggtcgat tcttttcaca acacccaaaa gcgtttcttt
    22381 tgccttaatc aggtcatcgt tttgcgattt tagaaaggcc tcccgttttt ccagactttc
    22441 atattcatct atggcagcca gattaacatt accaagccgt tgcagggcat ttctgctttc
    22501 ctgaatacgg gcttctcgtt cctgttcatc atattcatca acgccaactt ccttttcagt
    22561 taacgaggaa agcactaaat tgtattcagt ggcgatacgc tccacaaaag accggacacg
    22621 ctcttctttc tgggagcaat ccagttcgag tttatgcact tttttctgct ggatattttc
    22681 cttttcacgg ttttctttaa cctgaattgt gagtttgtca gtggattcct gcaaaaattg
    22741 aaggtcttta tttttaacga gaactgcctc atgagcgtga tctctttttt cggataaggc
    22801 tttggattgc gtgattgtgt tattaatttt taattcaaga tcggagatct gctttttaaa
    22861 tgcttctatc tgctgatgat gcagattggc tttttcaagc atttcatcac cgcctcggct
    22921 ggaacggtcg agatcccgct gcaattcttc ataggacttg attgtttcag ccagctcaac
    22981 acgctgttct gtattcaatt tagcaagagc agccagttct tctttttgca caagggctaa
    23041 ctgctgcgtt tcttcgaaat taccctgtat tgcctgatcg tctccgtcca tgcctgcaat
    23101 attagatctt gccgcctgca gacgttcagc aaggtctttt tcctgtgcgt tcagattttc
    23161 tttttgtttc tccaaagagt caagcatagc gcttaggttt gcttcttctg ttgcatgccg
    23221 tgcaagagcg acttcatgcg ctcccgccgt ttttctgata tcgttttcac tggcctcgat
    23281 ggatttcagt gcgtcagaaa gatcctgtat tttagccgca atacgcaatc cttgctcttc
    23341 aagcttttta atctgttttt tacgctgccg ggtggattct tccaattcct caatttcggc
    23401 actgcgcccc agcaatccgt ggctgtctgt tttggttcga cctcccgtga cggtgcctgc
    23461 agacgttaca acctcgcctt ccaaggtgac caggcgggga tggtgctttt cgcttctgga
    23521 tatcctgatg gcatcatcta ttgtctctac aagcagcgta ttgtataaaa gatactgaat
    23581 ggcaggcagc acttcagcgt cacattcaac gaattgaatc agagcaccta ccacgccttt
    23641 catattgtcg aatgaagcgg catcctcatt gcgtgccggt ctgattgtat ccagcggaag
    23701 aaaagtgaca cgtcccgctc tattttcctt taagaactct atagcactct tggcatcatc
    23761 ggcaagtcgg acgacaacat tgttaatatt tccgcccagc gctgcttcaa gagcaaaccg
    23821 gtactgtttt tctgttgaaa ccagatcgcc tgcaggtcca atgattccgg acagtccttt
    23881 aaattctctc tgttttccca tcattacagc acgcacaccc actgcaaatc cctcgtaact
    23941 gtcccgcagt tctataagag attttaatct tccttcttcg ctgctgattt tttcgcgcag
    24001 tgactgccat tcctgattga tgcgggcatg ttcctgattt aaatttcttt gcagcgtctg
    24061 gctctcagtt tttttagtga cgatatcggt aagattcgat tgaagaactt ttgctttttc
    24121 agaaaactgt tcaagaacgg tcctggcttc ctgatgacgt ttcttttgga cgacaatgga
    24181 ttcctcaaca gtattgcgct gcttatgaat atccgtaatc cgcgtattta cggtctcaat
    24241 ctcggtatgg gctttatttc ttacgttaat ggcttccaca cggcgctcat gcaatgcttc
    24301 tatcataatt tcggtgtctt gcagagcttt agtggcgttt tccagttctt ttgtcgactt
    24361 ttccaacgct tgctttaacg tttgaatctt agtggtatgc tcaagcaatt cctgagatac
    24421 ggacccggat cggtttttga gattggtgga ctcaagagtc aaccgctccg cttctgcttg
    24481 cgcttctaca gatcgagttg tgatgaactc aatttctttt ctatagaggg taatctggtt
    24541 ttccaaccgc tccatttcac tatccagaga atattctttc tctctgcatt cactgagtgc
    24601 acggcttgct tcatggcgtt taagggtgat cgcctccagt tctgcctcct gacggctggt
    24661 ctttgctgca agttcgtgat aatgatcggt ggcctctgac aattgagcct tgagatctgt
    24721 aatttgtccg cttaattcat tgtattgcag ccaggagttt ctgatctcaa gcagccgtac
    24781 ttgttcggtg agttctctat gcctgattgc agcgttgacc tgccgcttca aggtccgcat
    24841 ttggcggtcc acttccgcaa taatgtcagc caaacgaagc atattctgtt cggcagactc
    24901 gagcttgcgc atggcaacac gttttctggt cttatatttg ataatccccg ccgcctcttc
    24961 aaggaggtaa cggcggtctt ccggcttgga acttaaaacc agatcgattt ttccttgtcc
    25021 gatcagcgaa taagcatttg ttccgatacc ggtatccata aataattcct ggatgtcttt
    25081 taatcggcaa aggcttttgt ttaacagata ttcgctgtct ccgttccggt acacacgccg
    25141 tgtcacttca atctcagcat attccaaagg caatttcccg tctgcattgt caaaaaccag
    25201 cgttacttct gccatgccgg tggcggggcg ccctatagaa ccgctaaaaa tcacatcctg
    25261 catgtgagat ccgcgcagcg tttttgcgct ctgttctcca agcccccagc ggagtgcgtc
    25321 aagaatattg ctttttccac acccattcgg accgacaata gcggtagttc ccaagtcaag
    25381 agttactgta gtgggatctg caaaagactt aaaaccatga agataaatct gtttaaaata
    25441 catatctgcc agccagtcta tagagatgat tgtgaaagaa aagggactca atcacagtgt
    25501 tatttgttac agccaaccat ggtagtgcat accaaaatct ttgtcaggag tccagttgca
    25561 gtatttgctg aattcgctta acggagatag cgcgcccggt ccgatcatct gtttcaacaa
    25621 gaactgcatt aaactgcaca ggtccgtccg ctatttccca tttaacggga agtcccgtaa
    25681 tacttttttt cagaaccaac tcacttttta ctcccaggat actgttgtac ggtccgcaca
    25741 taccaacatc agatataaat gcggttcccc cggggaggat tcgctcatcg gctgtttgca
    25801 cgtgcgtatg ggttccgagc acagcagagc aacgcccgtc aagatgccag gcaagcgccg
    25861 ctttttcaga tgtagcttca gcatgaacat caacaaatat aaagggggag tcgcctgttc
    25921 gtgaatcgag ttcacgctcg atcgcatcga aagggcagtt agccggctcc ataaaaaccc
    25981 tgccaatcaa atttataagt gtacaatgcc ttccatcggg gagcgtataa acggctctgc
    26041 cttttcctgg cgcatgcgtt gaaaaattag caggccgggc aacagagggt agtgcatcga
    26101 tccctttaat catttcagga tggcgccagg catggtttcc caaagaaaaa agatggacgc
    26161 cctcagcttc aagctttttg attaaaggcg gagtcgcgcc tgtaccatgg gcaatgtttt
    26221 ccgcatttgc cataaccaca tcaatacaga gcgtttttat cagatcggga agcacgactc
    26281 tgaccgcttc gcggcccggt tttccgacaa tatcgccaat aaataaaaca agcatgggaa
    26341 taactttata gaaaaagagt ttaaaacgta ccgtacactg tcacatggga cggaaaatta
    26401 ctaacggcat acacataaag cagaaataga tgcagccagt ctttacactg aagcctgata
    26461 caagcaaaaa gaacaggcgc aggatcacac acgcccgtta ttggaacgtg gattcaatgg
    26521 attctgcaga ttttaaacga cgttgtcttt cttcatctgg catgccgctg caaagcccca
    26581 cttcactttc acgcagaagc gctttctttt ctgaaacaga aagcggtata tgcgcttcca
    26641 atttaatttc catgatcagg gaggaaaact cttcgaaaag agcgccggta tcccatcttg
    26701 ctttgtccag agtggcaatt gcttccagat aacgcccttc ctcgcgcaga tccagagcat
    26761 ccatcatgag cttgcgcccc tcaccgtaag ggtcgtccaa agcattccag gtatcccggg
    26821 cttttttcca atagttttcc gcctgagctc tgagctcttc ggcctcagca ggatccgatg
    26881 attctttggc tttttttctt aacgtgcgcg cctctttttc gtaaagcatt ccctcgttgc
    26941 gcataatgaa gcgtggagca acttccacag cagtcatgct gccgggatag cgttcgacat
    27001 aatctttcca tccttccagc gcatcaccgt attgaccatt cagttcctgg cacaaaaaca
    27061 gctgtctggg cacccacctg tcgtgaggca ggcgtatcgc ttccgacaga tatttaacag
    27121 cattgggata atcttccagt ttctgcttgt aaataccgaa tccaagatca aaatagagtt
    27181 tgtaattacg cgggttgcgt cgaatacctt ccctgaggaa gtcaacgcca taatagtaaa
    27241 aacgctcttt ttcgcctaaa cactgaccgt attttgcgtt ccattccctc tgtggccagg
    27301 gcgtgtcctg catttgcgcc gtggcattat aggataagtg ccaggcgccg acaagatagg
    27361 cttccacaaa ttgcggatcc agggcgacgc aggtacgcat aagtgtttgc atacggtaga
    27421 cattaccctg atgccagtac cggtcaactt caagccacac aagattggca gccactttcc
    27481 taaacccaaa aaacaggttg aatacattta caccgcccgc ctgtgcctct gcatattcga
    27541 tatcggtggc gaactgaagt ttttgctttt ttgcaagttg aagaaattca gcacgctcct
    27601 cttttgccag atcagattca gcaaaaaacc acagacgttt gtcattctca taatttttgg
    27661 caatgctgcc gatcatgctc aacccatcga ctttgtcaga cggggaaaag gatggaaatt
    27721 tttctgcaag cgctgtacaa cgtgcaaaaa aaagagcatc ttcggacgga tcctcatttt
    27781 cctgaaaaag acgctcatta acggcagcag ctaaaagcca acggtaaaaa gcctcagatt
    27841 ctcttaaatg ccgtaactgg ttgttcagaa ggacgccagc acctagagaa aacagcaaaa
    27901 cacccgccat aagggctgtt tggtatttct tgataaagtc cagcattaca cctccttgcg
    27961 acggaagaca aggtaagcgc aggaaaagcc ggcaagggcg tagaccagac tgtacaacac
    28021 gagccaggga atcagaacat ctgttggcgg gtcattgggc tggccattta caatctgcat
    28081 tttaatgctg aagttctgaa ggttgggtag aatggaatac aaggcagagg cgattttacc
    28141 gatcagatta tcgaaaaact gccctgtttg cccggcacgg tgttcaacgt ctttaagata
    28201 ttcagtcaga ttagcggtta tgtagataaa aagcccgccc aaggtgggaa caagcgggga
    28261 agtggctgca caggatacag caaatgtaaa agctgaaata attaaaaact caaagtaggt
    28321 aaggaaaatg ctccagagca acaaggttgg catgatcccc tgccgcatat aaagtgcgat
    28381 gaagaataaa ccacccatta acagcagatt gacaagaata attagctgta ttccctggaa
    28441 ttttcccaga agatattgaa cccggcgcac cggcttggac aagacggtgt aaatcacttt
    28501 tgtctccatt tctgctggaa caacggaagc agagatatat acggtgatta aggcgccgaa
    28561 aatagcaatt gccgtcacac agatatcttt gatcagctta agatcgatgt ctgtttccgc
    28621 actcgggtcg agaaaggaag aaataaaact ggagccaaag ataacaaaaa ggctcaggat
    28681 caaaaagccg ataagaagtt ttcttctgat accttccctg taggtgtaaa cagcgatgca
    28741 ccagggtcca ccaaaaacag atgataaaaa tgtcattgca ccacctcccg ggcttttgac
    28801 gctgtgctga caatgcgaac gaacaattct tccagggttt cacggcgtgg tgagatgctc
    28861 aagaggttga gggagtgtgc tttgcacatt tctacaccat cataactgga catgtgaaaa
    28921 ggcaggcgca aaacagcacg attgccggta cggtcttcca cttgaattcc tttatcagtt
    28981 agatcggaaa cactgctttc gctcagatga tcgacagtca tggaaagttc acgctgcgta
    29041 agcagttcag ccattgaacc gatcttttgc agcactcctt caaaaagtat ggcgactctg
    29101 tcgctgatca tttccacttc cagcaactcg tgactggaaa taagaagcgt aattccttgt
    29161 tcacgaagtt gaagcagcaa gtcgcgaatt tcttttctgg ctatagggtc taaacccgtg
    29221 gttggctcat caagaataag gattttaggg tcgcttatta acgcttgcgc aagaccgatg
    29281 cgctgccgca ttcccttgga ataaccccgc agcagtcgtt ttcgcgcatg ctgcattccc
    29341 actaattcaa tggtctcatc tactcttttt ttcagttctt tccctctaag accatacagt
    29401 tttccatagt aagccaggat ttcttcccct ctcaaaaaat ccgggtaata agccccttcc
    29461 ggaagatagc ctatgttcct tttcacggtg gcagagtcta tagactcgct tcccagaatt
    29521 tctatcttcc ccgaagtagg gaataaaagc cccagaagca ttttgattgt cgtcgttttt
    29581 ccggaaccgt tgggcccaag atatccgaat atttcgccct ggcgaatgtc cagatcaaga
    29641 tctttaaggg catgtacatc ctgaccgcga aatgcgcctt catagatctt gttcaatgcg
    29701 gtggtgcgaa tgactgttgt cacgtgaatt cagcctttcc ttagcagtat ggctcctgca
    29761 gcctgaaaca gaactgcaca aatgcaacta cagcggctcc agccacgctg tacctttttc
    29821 atataaccaa aacagtacca tggtagcaaa aacgcgggtt tgcttccaaa cagaactagg
    29881 catacgctct gtttcgggcg ttctcagccg ttgtcttcaa gaaatctttc gatttcagca
    29941 tgttccggga cttctccgtt ccaggacgca agttctctgc cattgcgcac aatcacaaat
    30001 ttaaacggat catcgtcaat aagcgtgaaa taaaggagag cacgatcgcc cagagaatat
    30061 gtgggaaaca ggggagagac cagcgcaaga aactcgtcaa ggtcgccagg tgaatcttca
    30121 taaacaaggg ctatcatggg gggaaggtcg ttggcttccc aaagtgcatt taattcaggc
    30181 acttttgact tgcattccgg acaggtcaca ctcattacag gcactaaata ggttccgtca
    30241 ctcagatcaa aatagccgtc atttgagaag atctgaaatt gtgcataaat gcccgctgcg
    30301 ctatcattgg aacgggtgag cgccgtaaag tcgccttgct gccacgcaat aaccaaaccg
    30361 gcaattccgg caaagcaact gagcacaaat ttgactgctg attttttaga tgcggcaaaa
    30421 gagaacagag aaacaggtga cagcgttttc caggaaaaca ggatagaggc gaaaattata
    30481 aggtttttga aaaggcttac cggcggagac atggcaataa cagggaagca gccacaatct
    30541 tttaaaccgt gaaaaatcca ggaatacagg atcatcccgg aaaacgcaag gatcattgcc
    30601 tgaacaagag cataaagcca tcctttgcag cgtaaagcaa gaagcaaagc cgcgcctaag
    30661 gaaaattcaa gaaaaacagt aaagaaggag gcggtcagaa gaaaatgcgg tgcttcaaga
    30721 attttataag cagccatctg gacggaaaac ttatcaatat cccacgcctt agggattgcc
    30781 ccggcaatga aatagaagcc gaccataaat tccagaaaac gcgcaattgt ctgttgtttt
    30841 atcttcattt gtattctaac agattgaaaa gagtcgtgtt aatgtaaaga aatcagcgca
    30901 ctttcgtgcc gttaaagact atttcaagtg aaccatgact gtcattaggt ttaaagtgta
    30961 aatccacgcc atccggccct gaggctttta tttccagcac actctcttta acggtccatg
    31021 aaacagatac cggctctttg ttgtaacatg cggtcgaacc ggaagcccag gtcaagccgg
    31081 taatatcagg gctgatagta aaagaagcac agccggtatt atcagaaaca atacccagga
    31141 tgattctatt caaaaaatga acaggcgccg aagaccaggc atgagtgtgg cttcgcgtgg
    31201 gaaatgcgcc tttataattc aatgcaccgg caaaggtttc ccagactgtg gttgcttcaa
    31261 tatcgagcat aggctgataa gcactgcgaa tacgctggat tacttcatgc cccaatcctt
    31321 ctttttccag ggcactgaat aaataaagga tggcaaaagg ggaaccgatg cgtgtcatat
    31381 tttccggcgg atccatgagg tgcgccagca ggctcgggcg catcctgtct tctgcaatat
    31441 caaaaagcag cgataaaaac gccgtatgct gagagtattt aggacttatt tttccatccg
    31501 catggatgct atccggataa gcgccggcat cttcctgcca cagggcattt aaagcgttta
    31561 caagaatgga acgccggtct ttcagccatg ctaaatcctc ttcctgccct agtacctgtg
    31621 cgatcttagc tgttgcgtca atggctccga ccatgaacat gctgttgtga aggacagtat
    31681 tatgtctgtc atctataccc gaccagtcaa acatattcca gaaaggcgcg ctgaaaagac
    31741 ccttttcatc ggcgtattct gcagcattgc gcagattttt catggcatag ggaaaagccc
    31801 attccataaa gtctttgtct tttgtgaact cataataatc ccgaatcatg atgttccaga
    31861 gaaaactcca aacaggaatg attgtttccc aggtggtcgg tacctgtgca agtaccatag
    31921 gatggtgatc cagcgaatga gctgccaatt tgatgcagcg tttggctata tcggcgctgc
    31981 caaaagccgt ataaccgaag agcgcctcgt tgcgggaatc gccaacccac agggtctgct
    32041 catagagcgg gcagtctgtg aaaacatcct ccatacacaa tttcagagtg tatgcggaaa
    32101 tttgccagat acgattcaac ccttcatcgc tgcaggcaaa gtgcccttct tcctttacag
    32161 gatacgttga ttcgatgagc ttgaaatttt taagtacggc ttctcttgtc tggttcctga
    32221 aagttagaaa aaggtaacgc tgtgaccggc gatttaaact cagaaaagta ttatcgccct
    32281 ccgagcaaat atagcgcatg ccattgcggt agcgttccgt atgctgcacg gctccgtccg
    32341 gtgcaatata ctcaactccg aacaggtcta gaatcaatcc ctcttccgca tggagttcaa
    32401 aggaatagta accgacattc tgggttccaa agtccaggat tatttctata tccccctttt
    32461 ctgacggctt tatgattgtg cctgatgagg aagagaggag ggcatccgca tttacaaccc
    32521 ggtcatttcc ggaagaaagc acttttctgt tttcaaaaag gtagtgggga gaaggggtta
    32581 tctcggcatc cgaaatggtg cgcatttttt tatcctgaac gaagcgggga aagctgtcca
    32641 gatccggtgc tgcgtcctta aagccggtga tacagtctga cacttctttt tctatgcgag
    32701 cctgttcttc tacagacaat aaagcccagg cataatcagc aaaacggtat ttcactttcg
    32761 gatcgggata gataaagcac caggaaccgt ttgcagcatc tcccagagga tggctcaggc
    32821 gaaatgcttc ttcgcaatgg ataataagtt cagaatcatg ccgccagtgt ccccagggct
    32881 ctgaaagcac aaaatagagc aagtgcctgc ccggctccac atgaaaggct ttgccattcg
    32941 cattctttcc gtcgaggtaa acctgattgg catccgggtt cataagaact tcgcacgccg
    33001 tatccacttc gaagacagtt gcataggcgc ctgttactgc aacatgatta ttggaataca
    33061 caatatcgcc atagagccag ttggttgtag ggaaaatgaa gcttttaacg ggcagctttt
    33121 ctacgacagt aacagcatga acagcggcag gcgactgaac aatccggctg agaaaaggac
    33181 agtcccgact ttcaaagttt ttccagggtc cttccagggc tttgcagagc accgtagcgg
    33241 gcacaaattc cttggcaggc tgccgcgcat cataaatttc aaaggggccc atctgcacgg
    33301 attgtttcgg ggtattttga atccaggcaa cagcgtcccg cacctgccat ctctcatcgg
    33361 tgcctacaga gaaatttgac ccgtcagaag ctgttacgtc aagttgagcc agcaagcctg
    33421 cctgaacagg aatctgatga aatgttccga ttccgtaaaa tttggcgata atatgaattg
    33481 tattttcgcc ttccttcaga aagccggcaa caggaatgac atcatattga taatgctcag
    33541 gccaggagcg gctgggcccg tctgcaaccc attcgccgtt aatcagcaat cgataagaag
    33601 tatcggcggt aattctgagc gttgccgcct tgattgccgg aagggtgact tcttttctgg
    33661 cctctatcgt gtcattataa caggcgtaat tgtcccgttt gttccatatc cacgatgccg
    33721 tccagcgtgc cgtatcagta ttttctgcaa aagtagcgtt catgaagagt cctgtaataa
    33781 ttaaaaaaac tattgtaaaa aggtaacggg acacagtatt tctccttgtc taagggcata
    33841 catatggact actgctttta aagcatgagg tacgatgact gatgatactc tatttcagga
    33901 agaagcgtct aactttgtac atccgcagcg agaagcgctg cttcaactgc ctgcctgaaa
    33961 ttgcgaagtc cggaaagcag attgtcctgt gccgcctcca gaaaattctg taaaaccagg
    34021 gttgaggaaa ccgtttcact ttcaatatgg catacccaaa cgatgtccgg tgctgccagc
    34081 gtcactgttt gcagagtatc cataaaattg cctaagggtg ctctgtccca atgatcgtac
    34141 tgaaaacggg cgataacgga cacgccgccg agttcggaag taccaagcgc aaaatcgcct
    34201 cctgcctgtt cgcatgcagc tttaaaaaag ctgagcccaa gccctgtttt tttccctttt
    34261 tttgtcgtaa aaaagggatc caaagcctgc tcgggagtaa caggaagacc gggaccgtca
    34321 tcttcaacag acagtgttaa aaaaccttct tttttaatca ccgcaattct aatgatcgtc
    34381 gcctttgccc gcatagaatt ttctataaca tccaacagat gaagagacaa ttcacgcaag
    34441 gctgcatctc cgaccgatgg ctcctgcaag agccagttta acttcctgaa aactcaaatt
    34501 cttcatttcc aaaaagcacc gcccgtctcc gatattatcc ggataatggg catctgaaga
    34561 agaaatcaag gtttcttttc ttgcagaaaa atccaatgct ttcccttgtt tgtaacctga
    34621 ggcactaatt tccagtgcat caaagacagg tgcaggcggg ataaatccca actgactgac
    34681 cacggagaaa gacgggcgat ccacatgggc ggcgacacag agaccgccgt atctccgaac
    34741 aagcagagca gcatcgtcca aggaaaatgc ggatgcaaaa gacaagaaac gatcggcgtg
    34801 cccgatgact tctccggcag cgttcagaat aggctgctct ttgcactgtt cggggttatt
    34861 tgccgcacag ggcagtgttt ttccgacttc atcggacgct gccaaggctg cctctgcatc
    34921 cggaaaaagc gcaaccaggt gcacttcttc gctcgtagta atttccatgc cgggcaaaac
    34981 aagaaataaa tcgccggcaa tggaaaaggc ggcttcctga accgcctgta tattagcggc
    35041 gctgttatga tcacaaatcg caataacttc cagatcagac gtaatggctt tatatacaat
    35101 tgcctcggga gtcatctcat ctgaggcgca aggtgaaagg gctgagtgta tatgcaagtc
    35161 cgcagcgaca actctcattc cttcagacct ctaactccat ttctgtataa cataccggcg
    35221 atttcaaaag caggaagaga ggaggaaaaa agatggatac cttcttcagc tgctttgaga
    35281 catgtgtttt cgtcgggacg acggttcatg gcaaagataa tggccttctg ggcagcgagg
    35341 gaagcgacag cgaccacatt cgcatgaacc tgtaccgtta tcagcactcc atcaccggga
    35401 gcattgccca taacatcact caacaggtcg gacacataag ctctttcaat tcctgcagcc
    35461 tcatctaacg gcagttgagg ggttaacggt gtcaagccta attcttgaac taaatctgtc
    35521 agcagcatta tttatttctc cgttgaggca ttttctgatg cattggattg cgaaagcacc
    35581 agaaacgggc attcatagtc cgtggtacgg gcaagaacaa tatcttcggc atgggtaagg
    35641 caacttggcg agccacaggc accgcaattg tttcccggaa aggttttagc gatttcatcg
    35701 attttattta atttctgaat cgctcttttc atgtcttcat ccaatcgggc tcctacccgc
    35761 ggcatcagcg gttgaaccct gtaaagggga ttgagctctt ttttcgcgcc ttccataacg
    35821 tcataatacc gtgaacgttt ttctgctatg gcgccgggcg ctgtccatac cggagagccg
    35881 taacatgcgc caacgcaggc ataaagcacc acaagccccg catcctgtaa gtagccgttt
    35941 tctatctggt ctagaaaatg gcacacctga ttaatgcctg ttacgacgag gcagtccttg
    36001 gcaggctgaa aggaatcagt ctgagggtgc tgagaaacag cttctttgtt tatccgtttt
    36061 tcaagaaggg aatggaaaaa acggggatga acagcatgaa ccgcattcaa ggcatggata
    36121 cctttggctt gagaaaattc tgccggacag gatggaacaa tgaccagatc gctgtcgata
    36181 atcttttctc gcaatgacag aagaggtgtc attaagggaa gcatatgcgg aagaagagac
    36241 gggaaacgca actgaatgag cctggaaata gaagggcaca agggtgctat tttaaactcg
    36301 ttattaacaa aacgttttct atagaactcc agatagccat tcatccacca ggtagccgta
    36361 tgactgaaga ttgtgttctc gactgtcaga ggtgagagga gacctcgaag cgcatccggc
    36421 gatgaaagca atgggaattg accgcgcaac gcttcaggaa gacaataaat ttgtccttcc
    36481 tcaaattccg ggagtgcatg atctgccttg aaatcaaaaa cgcctgtagg acagacacgc
    36541 atacatgccg tgcaatcaac acagaggtgc gtcagaattt cagggccttt cctgtggacg
    36601 cgcagtgcct gagtcggaca gacatgcacg cattttaaac aatggatgca cttggaaggt
    36661 tccattgaaa gtgctgtccc gaccgctccg tcggaactgt gcggcgccag agagatggca
    36721 aaacgcatca cagttccttt accgagggta gacgacatcg aaaaacgatc ggtgttgcgt
    36781 tggatattcg gaaggcccat tccggcgccg aaacccattt cgcgggcact gtcaggcgca
    36841 gtggaaaagc ctgcagtcat tgcctgttgc aagtcagcta tgcccagccc ctcgtcttca
    36901 atcgtaacgt ccagaacgtc gctggcaatg gcagcacgca cctttcctcc gtttgtatga
    36961 attgcaacat tcatttccgc ttcgtacagg gcaaccatgg cccggcggat aatggcagaa
    37021 tcgacgccta tctgtataag aagctctttg atttttcttg aagtccgccc ggcattggag
    37081 taattttctt tttctatgac ggcttctatt tcgtatttca taacgaactg gtgtcttctt
    37141 taaaaagaga ataaatgata ccgcagcttt catacatgcc gaaaggggac acaatcagag
    37201 gagtgcccgc attttccgcc gcttcctgca gctcagggca ggggggagtt ccattgagaa
    37261 gacatatggc agaggcatcc atcaactcaa taagacggat cataccctga ttcgaaaggt
    37321 gtgtgactat aagcatctgc tctgaaacag cgcccagcaa atcactcatt ctgtctccgg
    37381 caaaaatgtg atctatttcg catgtatcac tgttgtgatt tacgaagacg cgcgccccga
    37441 gggcgcttat aattgtagag agctgcatag caatcctgta gatgaagcgt aaaagttact
    37501 tctttgtgct gcggacaacc agttctctta ttttggacaa caatttgttt gcttctgccg
    37561 gtttagccat aaacgcatcg atatgcatgg cacggagatc gtcgtcattt ctgggaataa
    37621 aatcatagcc atgacgggtg gaagcagcgc tcatcagaat gatgggaagc tcttcatagc
    37681 ccggcagact ttttaccttg gctgccagtt caaagccgga gtcaagattg ttcatcatca
    37741 ggtcggaaac aagaatgtcg ggcctgttga ctttaattgc attgaaggca attgatggat
    37801 cgttgaaaca atcgacggta aaaccatctg cttcaagaat gatacggcac cattccagca
    37861 catcatcatc atcatcaatt acgactacgt acatgaaagc ttcctcgttt cattatcgct
    37921 tttcagcagc acttattgga aacattcggt acttatagac acaggacttt ctgcgatttt
    37981 attcttcttc ttcagaaggc aaactgacac tctttcctgc ttgcagtaca agcagcgttt
    38041 ttccgtcgga gacaataagt tttttccctg cagggtcata ttcactggct acccagtttc
    38101 cgacgacctg ctctccaaga agaaggctga cactttcttc tgttccgtcc ggatgctgca
    38161 gcacgccacg aaaacgcggg tctttgccgg gtacgtggac aacaccattc agatgaaatc
    38221 gcatttcatt atcacgtaaa ggggccgtgg cttcaccttc agctgaggac atgtcgtctt
    38281 cttctgatac gtcatcagca tcttctgtct cagcatccgt tataatttcc tgagaataag
    38341 tgcggggcaa agggattttt tctgacggat taggaggagt attagtaaca agattttgtg
    38401 aaagagtttt atcttgcgtg gtaaaagcag gggagaacag cgcattaaca tctttgactg
    38461 aaatgaaagc aatgaacaca gcgacagtaa caacaagaaa agtaataaac tgaaccgcca
    38521 tcttttcacg aattttgcgg tgaaccattg caataaaagc cgacatgttt ccgccgttga
    38581 tcagcgcagc tcctgccgga aaaaggtaaa gagcgaaatt attgagaacg gcagtatcgg
    38641 taggaacaac ggtgattttt ccttgcagat agaaaagact ataggctgaa gctgccattt
    38701 ccagcaacca gaacaacagg acgaaaggaa gggaacgaac ccagacactg cgccagagca
    38761 atagaagatg ggaaaataaa agaaatgaag gaagaagcgc cggaaggacg ggcagatcag
    38821 gatcgacaag atgaagaatg aaaaagaaac acaaaaaaga acaggtagcc aggcttaatg
    38881 caaaaatttg cacaatggga aaaaaagtgg tttcgttagc atcgggcgca ggcgcggcag
    38941 ttactcctgt tttcaaatct tcggggctgc tcgctttgaa aaatacttca agttcttcct
    39001 gccgtgctat taaatttctt gtttctgcct gtaacgcaga cgtaagtgat ttggtttttc
    39061 gccactcggc cttattgcgc gggatttcat ccagaagagt aatttgacat ttaatgaaag
    39121 atctgagatc caagtcgcaa tcttctatta ttttctccag acggctgcga caaaaatgga
    39181 tttgacagtg tgtacgggaa agaagtgaag cggcttcggt tgcaacggat tctgtagaag
    39241 caagcagttg cttttctgtt ttaatctgtt gggaaagccg gtctttattc tcaaaggaat
    39301 cttttaataa agagcaatag taagaagaca gcggggtaga gggatgaggc cattgtgaaa
    39361 attcaaaacc acccttgaat tgcctgcggt caaaatcttc taaagtgacc tcggcgggcg
    39421 gaaaaagaat cgcatccggg gaatattcaa accagcactc cagattgtac aattttctgg
    39481 cgagaaattc ccgctgtagc cgcagctctc ttacctgctg ttgataataa gcaagagcga
    39541 catcaccgtt ggaaagagac agggcaaaat ctgcaatttc ctgagcaatt tccgcaatct
    39601 tttttttgct gtcctcgatt tcttctgcaa gataattttt tatattgaga agcgcatttt
    39661 ctaaatccaa ttccagctgc tcaaaatatt cttcctgttc aggactttgg gaatgccggg
    39721 cccgattaag tgccttagaa tagcctgccc acttttgaag gggctcgaaa gaatccagtg
    39781 gaatattact ttcacttgaa taaacggaag cattcgattt tgagccaatc agtgtatcct
    39841 ggaaaaagag gtcaaattca ctagattcgt cttttttaga agcttcggtg gcactttctt
    39901 tcagaaaaga gattttcgag tttcctgctg acgtggaaga ttcctgcaga tcgctgctca
    39961 acaccttttt ctcgggaatg ttgtccctga cataaacgcc gggttttctg agcagttcga
    40021 gatgatctct caaataaatt attcgtgtgt cttcgccgat ttcgtgctct tcaggaataa
    40081 acaatttctg tttacaggaa acacaatcgg cagggcttcc atacattgac ggagccacct
    40141 gcattttttg cccgcatagg catacgatgc gaagattcgg tttttttata tctgtcataa
    40201 tgttctaaat gggatatcgt ttgatgcact tagcatatac actttatata gtatacatgc
    40261 ccttcacgga taaaaaaaga aaaggcaata aaataaaaat ccttgtaaac aacgggacgc
    40321 tggttacaag gataaaactt tacaaggcat taaagagcag gttcagaatc aactgcttcc
    40381 gttttctgag gcgaatcagt attagaatca gtggagacag gtgccggcgc ttcttcaata
    40441 cggaaaactt gctcacccgg gcgtgtcagc tggcgctcct gtctgatagt ggcttcgatt
    40501 tccgttggat cgctaacaag gctttgaact tttaaccggg ctgcagagac tttttcttca
    40561 agagattgaa tctcttcttt catgcgctct atctcgagtt cagtctgctg cagttccttg
    40621 tagcggctac tcatctcacg ttgttgcaca tagccatagc caaccagcac cacaagccct
    40681 aacccgatta gccatgtaac tatctcttta agcatgaact ttgtttcccg ggaggggata
    40741 ggatatcccc tcccgctgtt tctctgtcac ttatttgtgt ttaaaagctg cgagaccggg
    40801 aaaatacgcc tgctcgccca gctgctcttc gatacgcagt aattgattat acttcgcggt
    40861 gcggtcgctg cgggaagccg aaccagtctt aatttgtcct gcattgatgg caacagcgat
    40921 gtctgcaatc gttgaatctt ccgtttcacc gcttctatga gacataacag ttgtgtagcc
    40981 tgcacggtgg gccatctggc aagcatccag cgtctcagta agcgtaccaa tctgattaac
    41041 tttaaccaga atactattgg caacgccttc tttaatgcca cggctcagat agtccacatt
    41101 ggtcacgaaa agatcgtcac caacaagttg cgtctttttg cccagtgtat ctgtaagtgc
    41161 tttccagcct gcccagtcgt tttcgtgcaa cccatcttca attgaaatga taggatattt
    41221 tttaacccag tctgcccagt aggcgaccat ttcttcagag ctcagggagg cattttcggc
    41281 accgagaaca tacttcccat tttcataaaa ttcgctggaa gccggatcca gggctataaa
    41341 aacgtcttcg cctgctttgt acccggcttt ctgaatagcc ttcagtatga cttcaatcgc
    41401 tgcaacattg gactcaagtg aaggggcaaa tccgccttca tcgccaacag cggtatttaa
    41461 tccggcatct ttcaacacac ttttgagtgc atggaaaatt tctgtgccca tacgcagcgc
    41521 ttcgcggaag gaaggggcac cagcaggcat gaccataaat tcctgcacgt ccacattgtt
    41581 gtcggcatgc tgtccgccgt ttaaaatatt catcattgga accggaaggg tatgcgcatt
    41641 cacaccaccg atatactggt agaggggaag ctctaaagca gcggcagcgg cacgggcaac
    41701 ggcaagggaa atgccaagca acgcgttggc acccattttt cctttggtct ttgttccgtc
    41761 aaaagcaatg agtgtggcat caatttcgcg ctgtcccagg gcatccatgc cgattaattc
    41821 ggggccgagc tcttcattta cattgttaac ggctttaaga acccctttgc ccagataacg
    41881 tgctttgtcg ccgtcacgga gttcaacggc ttcattttca ccggtagaag cgcctgaagg
    41941 cacagctgct cttccactga aaccggactg taaaagaact tccacttcaa ctgtaggatt
    42001 accgcgcgaa tcgagaattt cccgaccctg tacactcatg atcctggtca ttgcttgtct
    42061 ctttctatgt tagattatgg ttacgaagct gcagacacgg tcactgctat tttaccttac
    42121 cacagggcaa ataagaagtg ctgcagtttc actgttacac ggtgtcgaca aattactgaa
    42181 ttgcgtcccg caaaattcta atttcactcc tctggcgcaa aaggttcatc cagcgacggt
    42241 attgctcttc agcctgtttg cgacgtaaaa ggggttctat gtcgattctg gcttcttcgt
    42301 aaggcgtagt gccctcggtc tgaatctctt caatacgtag gaagtgaaag ccgaaatcag
    42361 tctctaaaat gtcgctgagt tcattggcag gaagggagag gagggcgctg tttaagggtt
    42421 cgaccaaatc tccaggaagc acccagccaa gactcccgcc ttcagaagct tcggggccgt
    42481 cagacatttc ctttgccagg gcagtgaaat cagcaccgtc ttttatacga atacggatgt
    42541 cgttcagtct ttgtctgact tcatccctct tctttgcatc aaaaggggct ttcaagaaaa
    42601 accttctgac gagataacgg gtctcaaact taaactctga tttgtgatcc tgaaaatatt
    42661 gagccaaatc agattcacta atgattgcct ctttttcaaa ctgctgctgc ttgctcatcc
    42721 ggacagtata cgccatcatc tggcgccgaa tccgctctcg cagcgtggac ggtgtcaatc
    42781 cagttgcatt caaggcggtt ctgaaggctt cagcattcgg aaattgcttt tttaggtctg
    42841 agatgcggtc ttcaatttta tcgtcaggaa caacaacacc gagagcagtc gcctctttaa
    42901 agagaatacg aagttcaatt tcccgctcta acgcttcctt atacagcggg tttaagtgtt
    42961 gaaccatatc ttcttcactc atttcgagac tgcttcgatc tcccagctcg gaaaacattt
    43021 cctgcatgac atcgctgtaa agtacagggt cgcgcccgac agaggcgacg acgccatcaa
    43081 caaatgttgc agcggcagcg gtgcctgtag aagacaaact aatcagataa atggctatgg
    43141 cacaaaacga aataagagac ttaaagttct tcatatgtgc ttccaggtta tatctgcgtc
    43201 agttgttttc ttcattgtca gctgtttcag ttgagacatc gctctcaata tcaaaatatt
    43261 catcgaaatc ttctttgggg aatttcgctg tagaattctg ataatggcgg ctgcctgttc
    43321 ccgctggaat taagtgccct atgatcacat tctctttcaa cccttccaga agatcgctct
    43381 taccgtggat ggcagcttca gtaagcacca tgctggtctt ctggaaagag gcggcagcga
    43441 tgaaactatt ggtggtcagc gccgcttttg aaatgccttg aatgatggcg gcggcttcgg
    43501 ccggtcttcc gccgcgcatg ccaatctccg cgttgatcct ggaatgaagg tttttgtcaa
    43561 cctcttcatg ggcaagaaac ggggtgtcgc ccggatcagc ctgaatgcgg acttttctta
    43621 acatctggcg gataatgatc tcaatgtgct tatcatcggt acgcacgccc tgcagtcgat
    43681 aaacctgctg cacttcgtta agaaggtaag ttctaacttc gtcctcgcca agaacttcca
    43741 gcatgtcgtc aagaatcaca gaaccatctg tgatacggtc tcccgcagaa atgcggtcac
    43801 ccgtatgata attcacatgc tttgcgggcg gaatgctgta ttcgcgctct ttacccacag
    43861 gcggaattac tttcagcttt ctattaccgg ctgctgtcgt gctcagttcg acggtacctt
    43921 caatatggct gataatagca ggctgctgcg ggttgcgcgc ttcgaaaagt tcttcaacgc
    43981 ggggcaaacc accggtaata tcttttgtct tggagaattg tctggaggtt ttgccaagga
    44041 ggtcgccagc gcacacctga tcattggtgc gcacctgaac ctgtgtgccg ctgccgactg
    44101 taacgaaagc aagcacgtcg cctttttcat ccacaatatt aatttgcgga tgcacatcgt
    44161 gcttatgttc cgttacgatc agttcttcac gtcccgtttc agcgttcagt tccagacggg
    44221 tggtaacacc ctccagcata ccctgaagct caattcttcc ggatttctcg gcgacaatca
    44281 ggtcattgta cgggtcacgg gtaaacagga tctggcttga ttttaccttg gcaccatctt
    44341 ctatctgcat acggcagccc ggggttaccg gaatacgctc cgcggttttg ccatcttccg
    44401 taaggatttg gatttcgcca ccacgtttga taacaacagt aaatccttca ctgttccggg
    44461 ctgtttgcac attggagaac tgaatggtac ccgctgtttt gcagcgttgg ctttcgcttt
    44521 caacggtctt gctggccgca ccgccaatgt ggaaggtacg catggtcagc tgtgttccag
    44581 gctcaccaat actctgtgca gcgataattc cgaccgcttc gccaagttca acctttttgc
    44641 ccgtggcaag attacgtccg taacaaaggg cacacacgcc cttgggagcg ttgcacgtaa
    44701 gaaccgagcg gattttcaca ccatgcagct gctcttcttc aatacgttta gcagctgctt
    44761 ccgtgatttc ctcgtttgcc tgtaccaacg gctcggactc gccggagagg tatatgtctt
    44821 ccagagcata acggccgaca atacgttcac tgagcggact gatgacctct gcttcttcga
    44881 tgagggcttc acggtaaata cccgccatcg tgccgcagtc ttccatttca atcgtgacat
    44941 cctgtgccac atcgaccaga cgtcgtgtga gatacccggc gttcgccgtt tttaaagcgg
    45001 tatctgccag acctttacgg gcaccgtgag aagaaatgaa atattcgaca acgctcagcc
    45061 cttcacggaa gttggacata atgggcgttt caataatttc accggaaggc ttcgccagaa
    45121 gtccgcgcat tcctgcaagt tgtcggatct gcgacttact gccgcgcgct ttggactggt
    45181 acataaggtg aaggccggta aagccctgct cgttttcttc gagcgctttg accatggtct
    45241 caccgacttc attgttggtt gtggtccatt gatcaacgat tttattgcgc ttttccttcg
    45301 tggatataag cccaccttca tacatttcgt tgattttctg gacactttct tcggcttcac
    45361 gcaaaagttt atatttcttt tccgggacaa ccatatcatc aatggcaatg gaaagcccgg
    45421 acagggtggc atatttgaaa cctgtttctt ttaacctgtc caaaagagcg acggtgcgtt
    45481 tgtgtccatg ccgctggtaa actttggcga tcacatttcc aatagaggtc tgatcatgct
    45541 gccggttcac atcttcaatc gtgacctcgc tgcctagcgc ttctgcaaaa agaacgcggc
    45601 caacggttgt atcgattact ttcccgttga aatggacttt gatggcagca tgtatatcaa
    45661 cgactccggc atcataggcc tgaaccactt cgatcggact tgaatagatc tggccgaaag
    45721 caagaatttc gccttttttt cctgtccagt tactttgcca ttcacccttt gcgccttcac
    45781 ggactttagt catccaggcg atgccaagaa caatatcctg gctgggcgtg acaataggca
    45841 gaccattgga aggcgaaaag atattggaag aagacatcat cagcagatgc gcttcaagtt
    45901 gagcaggggg gaagagagga acgtgaatcg ccatctggtc accgtcgaag tccgcattaa
    45961 acgcacgaca gacaagcgga tgcaggcgaa ttgcattgcc ctcgataagt acaggctgaa
    46021 atgcctgaat gccaagtcga tgcagggtcg gagcgcgatt taaaagaacg ggatgatctt
    46081 taatcaccgt ttcaagaata tcccagactt cgtccctgcc tcgggtaatg aaggcttttg
    46141 cggttttgat tgtctgtgaa atgccacggc tcataatctg ctgaataata aacggctcaa
    46201 agagttccaa tgccatccgc ttaggcaaac cgcattcatg cagcttcaac tcgggtccta
    46261 caacaatcac agagcgcccg gaatagtcga cacgctttcc gagcaggttc tgacggaaac
    46321 gcccctgctt gcctttaacg gtatcgctca aagacttaag ttggcgattg ccgttcttgc
    46381 ctgtaacggg gcgtccatgc ctgccgttgt ccagcaacgc atcaacagct tcctgaagca
    46441 tacgcttttc attgcgcaac acaacttcag gggcacgcat ttccagcatg cgtttgagcc
    46501 ggacattacg gtttacaaca cggcgataca tatcatttaa atcgcttgta gcatacaaac
    46561 cgccatccag caatacaagc ggtctgagat caggtggcaa tactgggata acatccagaa
    46621 ccatccacgc ggggtcgttt cctgattttc tcagggcttc aacgatttta agacgcttgt
    46681 tcgccttctt ttttgcctgc tgcgaggttg ctgtgtgaaa ttgcacctgc agttcagaac
    46741 tgagggcatt taaatccaga tccgtcagaa gggctttaat ggcttctgcg cccataagag
    46801 cggtaaaacg gtcaggaccg tatttttcgc gctcttcttt gtattcttca tctgtaagaa
    46861 gcttcatttt aggcagcagc gtatcgcctg gatcaacaac aatgtattgc tcaaaataaa
    46921 tgatgcgctc aagttgttta atggaaagat caagcaaatt cccgagggaa ctggggttgt
    46981 ttttcagaaa ccaaatatgg gtaaccggtg aggcaagttt gatacagccc atgcgctcac
    47041 ggcgcacctt ggattctgtt atttctacac cgcagcggtc acaggtaata cctctgtgtt
    47101 tgactttttt gtacttgcca cagccacatt cccagtcctt ggcagggccg aaaatgcgtt
    47161 cacagaaaag gccgtctttt tcaggcttgt gggtacgata gttgaccgtt tcagcttttt
    47221 tgacttctcc tctggaccat ttcaagatct cttgaggaga tgccagccgt atttgtatcg
    47281 cgtcgaagtg ttcccctgtg gtgggtacat atttagccac ttgctagtcc tccatcgtat
    47341 aatcatcttg atccgaaaga gactcggatt taaccagttt cacaatatcc agacataacg
    47401 cctgcatttc gcgcaccagc acgttaaagg attccggcgt gctcggttct atctcatgtc
    47461 tgcccttcac tatcgcttca taggctttgg ttcggccttg aatatcgtct gacttaatgg
    47521 tcagcaactc ctgcaaggta taggcggcgc cgtacgcctg gagtgcccaa acctccattt
    47581 cgccaaaacg ctgaccgccg gactgtgcct tcccgccaag gggctgctgc gtgacaagag
    47641 aatagggacc aatcgcacgg gcatggatct tatcatccac gagatggttc agcttcatca
    47701 tatagatctg acccaccgtt gtctcatggt gaaaataatt accggtgcga ccgtcgcgga
    47761 gtcggatttt cccattttca ggaaggtccg ctttcttcag atattcgcgg atagtaccct
    47821 cgctcacgcc attaaatacg ggcgttgcgg ccttcatgcc caacgctcct aatgcccagc
    47881 ccagttgtgt ttcaagcaat tggccgacat tcatccggga gggaacgcca agcggattca
    47941 ggagaatctg cagaggagta ccatcaggaa ggaagggcat atccgccgcc ggtagaattt
    48001 tagcaacgac acccttgttt ccgtgacgtc ctgccatttt gtcaccgacg gacatgcggc
    48061 gtttggtggc gacataaact ttaacagtct tattgacacc ggcaggcaat tcatcgcctt
    48121 ttcggatcct ctctatctgc gcatcgcgga cagctaccag tttcgcctct tccatggcgg
    48181 caagatttac aagctttacg aactcttttt ggattttctt atcctgaact ttcagctggg
    48241 caagatagga ataatcgtag ctggacagat atttgggccg gatttttttg cctttttcaa
    48301 aaataagatc accggaaacc atgtcataca cgtctgccag aatctgaagt ccaaccatct
    48361 tcttgcttac catctcttcg aaggtgcttt tgatttcggc aatgcgctga ttgaattcct
    48421 gaacgatctg tgtttcttct tttttcatcg cccttttggc ttgcgtatcc gagccttttt
    48481 cccggcggct gcagatttta acatctacaa taacgccctg ggtgcctggt tttacataca
    48541 gggatgcgtc tcggacatct tccgctttct cgccaaaaat ggcacgcagc agtttttctt
    48601 ccggagcaag ctcagtttca cctttaggag tgactttacc gacaagaata tcaccggggc
    48661 cgactttagc gccgatacgg acgattccgt cttcgtcgag atttctcaga atttcttcac
    48721 cgacgttcgg gatgtcgcgt gtaatttctt caggtccgag tttggtatcc cgtgcctgca
    48781 tttcatgcat ctcgatgtgg atggatgtga aagcatcttt ttcaagaagc tcttcactca
    48841 ggataatggc atcttcaaag ttgtagcctt cccaggacat gaagccgacc agaacatttt
    48901 tgccgagcgc cagttctcca tgatcaacag cagggccatc agcaatgatc tgtccgggca
    48961 caactctttc atttttctgt acgatcggtc tctggttaat gcaggtgctc tgattggaac
    49021 gagaatattt cttgagctta tgcacttcct caatggaatg gaacggatta tcgcccttct
    49081 tttccttgac cctaatacgg attacttcgc tgtcagcgta ctcgaccaca ccttcccgtt
    49141 cagcgcgcac aacggttccc gagttgcagg cggtaatgtg ctccagcccg gtgccgacta
    49201 taggtgattc gctgcaaaga aggggaaccg cctgccgctg catgttggaa cccatgagcg
    49261 cacgattggc atcgtcatgt tccaggaaag gaataagagc cgcagcaacg ctgaccatct
    49321 gcttgggcga gatatccatg taatccacct tcgcgggggc gactcgcggg aagtcgtcct
    49381 gaaggcgaca gagaaccagt tcttccgtaa agtttccttt ggcatccaaa ggtgcattgg
    49441 cctgcgctat cgtgtagttt tcttcatcaa atgcagaaag ccgttcgatt tgatcgtgct
    49501 gaacacgtcc gttttccacc ctacgatagg gcgtctctag atagccgtat tcgttaatgt
    49561 gcgcatatgt tgccagagaa gccataagcc cgatattggg accttccggg gtttcaatag
    49621 ggcagatacg tccgtaatgg gtaggatgca cgtcgcgcac ttcaaagcct gcgcgctcac
    49681 gggtaagacc gccgggaccc aaagcactca aacggcgctt gtgggtcaat tcggccagag
    49741 gattgatctg atccataaag tgggacaact gactgcgacc gaaaaattcc ttaatggcgc
    49801 tgttaatcgg cttaggattt acgagaccct gaggattaag attttcttcc tcattgtagt
    49861 tcatgcgatc ccgggcggtt cgctccatgc gcgtaaggcc tttgcgcatt tcattgtaaa
    49921 gcaattcgcc tatggaacgt acccgccggt tacccagatg gtcaatatca tccagatacc
    49981 ctttgccgct gcgaaggcgg gacagatact gcatggtttt tacaacatct tcagcagtca
    50041 gtgttttcgt gtccggatcg atctgcagtc caagtttacg gttgatttta tatcgcccca
    50101 ccgatgcaag atcataacgg gatggatcgt gaaaggtctt gttgaagtgc gagcggcatt
    50161 gggctgtcgt ggtaggatct tcaggccgcg aatgcgaata aaaggcgtga agcgcctcat
    50221 cttccgtggt cgtagcatcc tgctgaagcg tattgacaat agccttgtcg ttaatcagat
    50281 ctttttcagc aatcagcttc agttctttta cggaagaagc gacaatccgg ttcagcttgg
    50341 cctgtgtcaa ttcttcaccg gcatcagcca aaatttcgcc cgtttccaga tctataactt
    50401 ctgcacccag aaaacgattt tcaaggcttt caagagggac ttttttcttg tcaatcttcg
    50461 gatgtccccg ctcaaaacgc actgtctcca ttgtatagaa gatctggaga atttcgtcat
    50521 gatcaacaat gccaaagcat ttcagaaaag tggtcgccag taatttgcct tttctgttga
    50581 caataaccca aagagtgtcg gatacatcat attctatttc aagccattct ccacgattag
    50641 gaattatccg ggcgaacagc aactctctgc cattggtatg ggttttcttt tcaaaagaaa
    50701 caccaggcga tttatgaagc tggctgacta taaccctctc agctccatta atgataaatg
    50761 tacctgtttt ggtcataaca ggcagctcac caagaaacac ttcctgctcc gaaacaagtt
    50821 tttcctgctt ttcgttggtg ttcccgatat cttcaaagct ggaaagacgc aatacggctt
    50881 tcaatgaagc ggaataggtc agcgcacgat caagacactc ctgcacactg tatttaggag
    50941 gtgtcaagga ataactgaca aattccagcc gcatggaacc gttaggggag acaattggga
    51001 aaacttccga aaaaaccgcc tgaagaccct ggcactttct ctcttcgggg agcgtttccc
    51061 actgcaaaaa gtcagaaaag ggttttgttt gaatttcaat caaatccggc aattgatagt
    51121 catcatggat tttgcctagg aaagtacgtt ccgtgggggg ggcaacagcc atttacagca
    51181 ctccttgcta ggtgtttgat catcggcgca acgcttgtgg aatggggaaa aaaaaacctg
    51241 acacactgca cccagagaca gttattgtta ttttaacaaa aatcgtgccc aaaatcaaaa
    51301 ttg
//
LOCUS       DAOPWB010000006        46101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_735_length_46101_cov_5.683056, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000006 DAOPWB010000000
VERSION     DAOPWB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 46101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46101
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_735_length_46101_cov_5.683056"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKN92_01365"
     CDS             <1..653
                     /locus_tag="PKN92_01365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ47470.1"
                     /translation="TERRKNLLMESDIRHLPETDCRKAAKALARSFRNDPGCLFVFST
                     PERCEKGMRRIFRSWLRIMATHKACWTVPDCSGVAVWYSSAAKPALACAALFKTGLWR
                     LPLYMTPAEQYRGIRFFRDLKKRTENEETEEHWVLDTLGVAPEAQGKGIASALVRQGL
                     QHADKAGKPARVITHNPANVPFYRQFGFEPVESGCIAGTEVQVIVMRRKVSDTVCC"
     gene            711..1322
                     /gene="pgsA"
                     /locus_tag="PKN92_01370"
     CDS             711..1322
                     /gene="pgsA"
                     /locus_tag="PKN92_01370"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNZ47471.1"
                     /translation="MNLPNTLTVARCILAGIFVVLMSFDHNLCYFIAYLVFILAALTD
                     YFDGKIAREQNLITNFGKLLDPVADKILMVAGFIMLMRVPDLTIPAWTVVAILAREYL
                     ITGARTLAASEGAILPANVWGKAKTVIQMAYVFVVLALVILLRFVFSVPALRDAIPYT
                     REMCAQTLHYLSFGGIILVSIYTVVSGIQFTWANWNALKLHDI"
     gene            1338..2477
                     /gene="alr"
                     /locus_tag="PKN92_01375"
     CDS             1338..2477
                     /gene="alr"
                     /locus_tag="PKN92_01375"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNZ47472.1"
                     /translation="MISRHPSRAIVDLNAYASNLTLVQRFAGKEIALIPILKANAYGH
                     GAIPLARKALEANPLLLGVATVEEGIQLREAGIEAPILLMLQPNQEALPLCIQYRLTL
                     MISDTGLAETLGRLAAQTNTVVPVHIKVDTGMGRQGVSLADALQTIQYISRIVHVDIQ
                     GICTHMSTSEIQDDGFTHGQIKSFKALLKQVDKAGVPYEVTHAANSAAIVNYRKSILD
                     AVRPGLITYGVWPSREKNATPQLKPVLRWETRIVYLRDLAPGSTIGYGRTYTTFGNTR
                     TAILPVGYADGFRHSLSNKADVLIRGRRCPVRGAVSMDQIVVDVSALPNVAVGDVATL
                     IGKDGTQEITAEEMAAKAGTIPYDILTGIGTRVHREYLHETKTDH"
     gene            2553..3104
                     /locus_tag="PKN92_01380"
     CDS             2553..3104
                     /locus_tag="PKN92_01380"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNZ47473.1"
                     /translation="MLDHYFIDAYNVIHKSSYLRQQAQRDLELARETLLEMSFAFSLV
                     GQNEITVVFDGKELQYDIPILHRLGNAPNLHIFFSPHGTTADTVIERKIYQAQNRMRC
                     IVVSNDRSLREQCRGMGAMTMEADSFISTIKQMQQRTQEQLSSRVKSRATLIEDRLNS
                     RSLEALAAIREEIVSAGDKRKKR"
     gene            3242..3784
                     /gene="rsmD"
                     /locus_tag="PKN92_01385"
     CDS             3242..3784
                     /gene="rsmD"
                     /locus_tag="PKN92_01385"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNZ47474.1"
                     /translation="MTLHIIGGSAKGRRLFSPPDHNTRPTAARVRESLFNILQDIIEG
                     SHFLDLFCGAGAVGIEALSRGALHATFVDQQADALDLVRRNLRLCKMEGQADLARLQL
                     PGKLSRLTTPYHIVFADPPYALPCQEQLLRALCTHRLLCPDAIVVVESGKKAVLPDSV
                     ESLARKDTRTYGDSVLHFYS"
     gene            3896..4378
                     /gene="nrdR"
                     /locus_tag="PKN92_01390"
     CDS             3896..4378
                     /gene="nrdR"
                     /locus_tag="PKN92_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNZ47475.1"
                     /translation="MRCPFCGFDDSKVVDSRVSKEGDSTRRRRECISCTKRFTTYERI
                     EEVAQMVIKKDGRRENFDRWKLKSGIMKAIEKRPVSLEQVDALINDVERELYNGGEHE
                     IFTRDLGEAVMTKLRYLDEVAYVRFASVYRQFRDVNEFMLELQDMIPFAPPGKTGKKA
                     "
     gene            4476..4862
                     /locus_tag="PKN92_01395"
     CDS             4476..4862
                     /locus_tag="PKN92_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ47476.1"
                     /translation="MAYDKTILVVDDEPDVVSLLEATLKGDGFNVLTAYDGIAALDLC
                     STEKPDLVLLDIMMPMMSGYEVCEQIKANPMTQQIPVLCISSAHTPEARAQSIRVGAA
                     ELIKKPFLPKELIAQIRRHLPKENDF"
     gene            5088..6512
                     /gene="der"
                     /locus_tag="PKN92_01400"
     CDS             5088..6512
                     /gene="der"
                     /locus_tag="PKN92_01400"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNZ47477.1"
                     /translation="MKARRKKTLPLVAIVGRPNVGKSTLFNRIIGKQRAIVHDIEGIT
                     RDRFINEAVWKDCPFRLVDTGGLVENPIDEITRKMQEQVFHAIEEARAILFVLDGQQE
                     ITRADEQVAELLQKTGKPVVLAVNKLDNHQLAMNQYEYYRLGLGDPFPISSGHNLGIE
                     ALMDALVTHLPAPPAEEAKAAPEDGDDDNETTDDTEETDTADEPQPIKVAIIGRPNVG
                     KSSFINALLNEERTIVTDIPGTTRDAIDIEFHWHGKDYILIDTAGLRKKAGIRQHAEH
                     FSVARSLRAIRRADVCLVMMEATEGMSEQDKRIIGYALEQGTAFILVWTKWDLIENKE
                     RRFKALEDDLDLKMPQIQYAPRLTISNITRKRLFSAFEIVDRVAGAAKKRVGTGELNR
                     LIEEIKTKHNPPSQKGKHAKILYATQASVQPTTFVIFVNQKRLFHFSYLRFIENQIRE
                     RWAFEGVPLRLELREEKRKERKPR"
     gene            6509..7087
                     /gene="plsY"
                     /locus_tag="PKN92_01405"
     CDS             6509..7087
                     /gene="plsY"
                     /locus_tag="PKN92_01405"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNZ47478.1"
                     /translation="MTIPLAILLSYLAGSIPTGLWLGLRFRGIDIRKEGSGNIGATNT
                     LRVLGKPFGAVALAVDVFKGWVAVTVFSLLNPWLYLPIICGLAAILGHTFSLFVRFRG
                     GKGVATSAGAFLGLAPMPMLIAGAVFLVIVGITRMVSLGSITAAAVAAVSVFFFPVSG
                     PVRIVAVLVAALIILRHHSNIKRILKREEPRL"
     gene            7091..9055
                     /locus_tag="PKN92_01410"
     CDS             7091..9055
                     /locus_tag="PKN92_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNZ47479.1"
                     /translation="MPGSTASMQQVDAMLSSPDPDSKPFFTAADLVIAFVGGLAALTL
                     YLLTLAPTISGEDSGEFITAAATLGIAHPPGYPLYCLLGHLFSWMPLGTPAWRINFMS
                     AFFSAASISLAILTVIYLTRNRRAALMAGLMLACTRDLWSQSVVAEVYTLTLFVLAAS
                     FLTLLLWSDRGESRYLLFFAALCGTGITVHNTMLLILPPAALFFLHSEYKAKERTSPA
                     LLFYACCCAALPFLLYLYLPLRSRANPLLDWGNPETLPLMLRHIFRSQYAFMVDQYPR
                     DLGRFLEQLRVFLRFAREQAGLIPAVCGLTGLLLLLRQRKDYALFLLFSLLLIPAAFS
                     FWQNFEITREWLWVMRVFGIPSWYILALGCGIFLHRLSETSQKMKPFFWLFAVSFILF
                     PLVRNYRYNDKSDYYWVEDYARNLADHLEPGALFISESDHASFSLLYFQAVLGERRDV
                     ENIRRYGYLQTPLFDGLPKELKEKTGAFPPRRYDAELIAWLLDHTTRPLYVARPRPLP
                     SQLFARYEACGPVFRALRHGENFSLPDTIRTDRRNRSDDTRGDYTADAILYEESMNRA
                     LRLFLSIRQDGTNPESVKAEVLEAVEKGLYHYTRDAAALNNVGTLVARCGYQAEALAY
                     FEEASRLLPHSPEIRKNLQRMKATGDLPAR"
     gene            complement(9068..10918)
                     /locus_tag="PKN92_01415"
     CDS             complement(9068..10918)
                     /locus_tag="PKN92_01415"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNZ47480.1"
                     /translation="MRFFLTAFSVLYCLSLASGAAAQSSSDWVADSIERVFQRFDEVQ
                     EPAFTDDFILADVKLDPEYPRRFDEFSGDISGRFIGAVAMASRGEEDDARLQQLMEKI
                     LPCQREDGRFGNASLSFDAADIGPDQMALLWGNGRLLVGLLEYAQRFPEEKAVIEAAV
                     KLGDFLLKVFDECATEEVMARVRGKAANGFICFTQFNEGLELLARATGNEHYRDSAKK
                     MEALLEPRGVQHTHGYLTTLRGHMMIYESTGDPALLSAVEARFDDMEASGSILITGGL
                     LEFFKEDYDRDEGCTEADFLRLCLQLWRATGKEQYLARAEQCLNNIFYANQFETGDFG
                     HRFFNNQGYIPHPGAGRAWWCCTMHGLRALQDLHDYDITPVERDGKKGLYIALWQETA
                     CEDGDFRAKVERVILPEKPYMLAFQIEVIDAPQDTRVLSLRVPGWAKDLSATVSLDGR
                     TVGNTISQQPSAEAASQLISRSISSLDLERVWQAGERVTVGIECALRFADRQGQVHLL
                     DGLPENGAEERAVFFGPWLLGVDAHRNPMFHGEPYSKNILLLPKDQEELRKHVSMHSD
                     LPGAVQAPEVTLSYIHDGFPDRCTATLQPVAAQNARPQTTVSYWHWMRRQ"
     gene            complement(11023..12306)
                     /locus_tag="PKN92_01420"
     CDS             complement(11023..12306)
                     /locus_tag="PKN92_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47481.1"
                     /translation="MTRTVQKYLRTRSVHSQFKLLGASPPGHVSAVIVIPALAESQHL
                     GTTLAALAHCSTETLCRSLVICVVNNRDENHSDPSDREDNRKTLLQLEQWAQDGYCAP
                     LTLNWIDASSPGQCLPAGEGVGLARKIGLDHGLALLSRNDCAFGAPRYLVNLDADSHA
                     LDGYLDALHSYYASAKRHGGYAAYAHRLEGSGQVQEAMIAYELYMRYHELHLRWAGSP
                     YAWPALGSIISCTADAYAAAGGMNRHCAGEDFYFMQQLRKTGTLEQIPHATIFPSGRL
                     SSRTPFGTGPHLKSGAARRYLHHPEAYQILKEWLALPGDNAALSPERLLLRSEELSPL
                     LMQFLTAKEFPRHWEKIYRQHGRGRQLHRHFHSWFDGLRTIQWLHYFRDHGKADLLAK
                     EAFTVLQQRISFPEKSLQQGNRELLETLRLWKYTG"
     gene            complement(12309..13208)
                     /gene="miaA"
                     /locus_tag="PKN92_01425"
     CDS             complement(12309..13208)
                     /gene="miaA"
                     /locus_tag="PKN92_01425"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNZ47482.1"
                     /translation="MVIAGPTASGKTGLGVQLAALLDGEILSADSRQVYRGLDIGSGK
                     DLNEYSFNGRSIPYHLIDIADPSDEFSLFHYQQAFYPALASVQERNKLPVIVGGTGLY
                     LDAVLSGYAMVEVPPDKTLRAALADLEMPQLIARLESTGKNLHNQTDTEDRERLIRAI
                     EIAEYTASHPAPPLPKINAFIMVIDHDKEVLRERIARRLKERLDQGMIEEVAQLHESG
                     LSWERLELLGLEYRYVAQYLQGAILSPNDLFQKLNSAIARFAKRQRTWFRGMERKGHV
                     LHWLPDAELGTALNAIKYAVRQP"
     gene            13351..15000
                     /locus_tag="PKN92_01430"
     CDS             13351..15000
                     /locus_tag="PKN92_01430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNZ47483.1"
                     /translation="MFFSRRLQRCLFYILLPFLSVFQSQAEEERFFADYFYLAPETAT
                     DKSAQDPAYARRYPMPERQVRRYVAADKGRLHFSEFRHDYFMEEKRADVEPTQIALGK
                     SVNEKAPDFFAQVPSEKGMHIFLAELSSEDAAALRLRVNLCALQPGEELWLLDSRGDA
                     AFGPFTQRHARPEGNWLPTTAGDRAVLLLRTPRKECPLLEIQSYSHFFKSIFNPSLRD
                     PKPCHLDASAAEDPRAQEISTAVGILIIPRGNTQSFCTGTLINSLGHDDATLPEPYLI
                     SAGHCFEGGPDVAGMEVFWDYRSETDDPATLPRSSGASLVHVNHVLDGALLRLEGEVP
                     SGPFGRAWAGWDSLRPVRQSLVQCFHYPVASSMKLSRGLISLAKTDVCKNILCTSSFE
                     RQIKINWYEGIAEAGSSGSPLLLPDFNFRIIGMLSNGNDHSCRSSQNNFDHFAAFHEY
                     FPEIKCHLVPGEKCADPVGRTASGCFLRRLFCLNSETLADLYRFRSGILEKSSWGRSC
                     IDEYYSLAPVLENWFNLDPLAKEAFGLVVEAAAFWGHLLQD"
     gene            complement(15091..16719)
                     /gene="groL"
                     /locus_tag="PKN92_01435"
     CDS             complement(15091..16719)
                     /gene="groL"
                     /locus_tag="PKN92_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151544.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNZ47484.1"
                     /translation="MPKQLEFGEGARRKVLDGVTQLSRAVRVTLGPKGRNVVLDKKWG
                     APTVTKDGVSVAKEIELEDKYENMGAQMVKEVASKTSDVAGDGTTTATVLAEAIFREG
                     LRNVTAGANPMSLKRGIDTAVSAVVEKLAGMSKQVRDDKDVIRNVASISANSDAEIGN
                     IIADAMDKVGNDGTITVEEAKSIETTLDVVEGMQFDKGYLSPYFVTDSENMSAVLEEA
                     YILIYEKKISSLKDLLPLLENVAKSNKPLLIIAEDVEGEALATLVVNKIRGTFQAVAV
                     KAPGFGDRRKAMLEDIAILTGGLCITEDLGRSLESVEMADLGRAKRVVVDKDTTTIVE
                     GAGASADIVGRINLIRKQIEETSSDYDREKLQERLAKLAGGVAVINVGAATEIEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALLRCQDAIDGLALEGDEKVGAAIIRRALEEPM
                     RQLAINAGDEGATVVQNVRAKKGNVGYNAATGEYEDLFKAGVLDPAKVTRTALQNAAS
                     IAGLLLTTEVLIADLPEPEKAPAGPPGGDMGGMY"
     gene            complement(16756..17046)
                     /locus_tag="PKN92_01440"
     CDS             complement(16756..17046)
                     /locus_tag="PKN92_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNZ47485.1"
                     /translation="MTVRPLADRILVKREEPSETVRGGIIIPDTAKEKPQEGIVIAVG
                     PGRIDDQGKRVALEVKKGDRILMGKYAGTEVKIDGVEHIIMREDDVLAVIEK"
     gene            17405..18490
                     /gene="menC"
                     /locus_tag="PKN92_01445"
     CDS             17405..18490
                     /gene="menC"
                     /locus_tag="PKN92_01445"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNZ47486.1"
                     /translation="MIIRDARSYTYSLQLKAPLRRKEEVVVRRKGVLFFLQGEEDFIG
                     WGEAAPLPGFSRITLQEAQALGRAAAKAVTGRSIADARNWLTPEQSGDPSVYFAFHQA
                     FRVLAQEHEEEKKDSCTVALCSLLDGDLNETEARLQAGLAEGCRCFKLKVARRSLAEE
                     TDLVAHILSQLPSDSTLRLDANRGWTYDEALAFCCSVPLEKIAFIEEPLQDWRQIPRL
                     QTESGIACAVDECLQDMSALLLFRADHDREALMHAYRTVVEAACYLVWKPSLSFPPEA
                     LGLDIRKRCILSGAYESGVGTAAVLEEALRRGGASLAAGVDTYSRLAEDLLLTPLHLE
                     GNRADARLFIPAGQAVDVTHLEECCCE"
     gene            18483..19928
                     /locus_tag="PKN92_01450"
     CDS             18483..19928
                     /locus_tag="PKN92_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ47487.1"
                     /translation="MNKASALSCPIREQALKHPRMPALCDAGGVQDYASLDAAIEQYR
                     AMLGAQHCKAGDRILLTGTPSSAYCALLWACFREGITACPVNSALPSAAMEHWARFTG
                     AAAVYGEHADLLPPSLPRLLFSGTFQETEEENPGKKLPLNGLQHAVIIQTSGSTGTAR
                     GAVLTLSAVLAAAGFANQNMPLRQGDRWLVTLPFFHVSGLSILFRCAAAGACAVFPEA
                     GSTLAQGLLAHDATHLSLVPTQLQRLLDDPEAVPCLRKMKGVLLGGAPMPESLLRRAF
                     EAGIPLIPSYGMTETAAQCCAVPPGSDFSLLQTSGRPLQPGSMRIDERGMIQYNGDAL
                     FQGYLQGDGSLHLPLSTDGWFPTGDCGFFDEAGFLHVNGRADSMIICGGENIHPEEIE
                     QLLCTAPGIRRVLVSGVTHAEYGTIPVAFIDPEDGAPIDEVFLRRYLAERLPRFKHPR
                     RFLRWPDAEPGGLKASRSWFASEAERLLEEK"
     gene            20069..21634
                     /locus_tag="PKN92_01455"
     CDS             20069..21634
                     /locus_tag="PKN92_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47488.1"
                     /translation="MVEKQILSGCACCGISRRHFLAAGCAACAGAAGLALPVRRVYGA
                     GNSSKGTLRVLFALHAETQPGPDWPNVGFEFRPVMDRLMTQLEKECGEFQYLPAMVNG
                     AEATQAVIDEDKGKDILGYLVFQLNCWNPVAQTAATQGKPVLYVDFKYGGSGGFLVYT
                     AHFLRTQSGNVGFVASSDPADLTAAVRCFNAVAEGAPASGFAGLVARCRQERTAARSD
                     TACLNDPVKTLPVAEWKERIAGSKILLYQDQESADLGTYMGIPLMKRPFAELNAAWEK
                     ADREESAHLAEKWVREAKAVEGVPMEVLKQSAAMYLANKALLKEHGANAITINCLGGF
                     YGGHIHAYPCLGFHELCNEGLVGACEGDVRSTATMTAFGILSGGRPGMISDPVIDTSK
                     RQIIYAHCVASKRVFGPQGSDNPFTILTHSEDRQGAAVRSLLPSGYLTTTLEVNPDEK
                     EILMHQAKAVDNDPDDRACRTKLCAEPLGDMEKLFTQWDRYGWHRVTFYGDLREAVHE
                     LADAIGYQVLEEA"
     gene            complement(21662..23149)
                     /locus_tag="PKN92_01460"
     CDS             complement(21662..23149)
                     /locus_tag="PKN92_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ47489.1"
                     /translation="MTKKQVYSFIPAMLLALFLSGCVHSTLPLTKISAPMPQSPVTLS
                     TVKALFGGYSDGNFSGRSWEAAFDAMHQKLSREYAFTEWKGVDWDALYDRFKTRLDEA
                     SANNDAEAYYLTLREYIYSIPDGSLSITTPQEYKESHIGGGYGFSLLPLEDGRVVIAW
                     LDPESQAAVTGIQWGAEILEWNGLPINEALKQQPVLWNDVPVATLENEKFVQCALLTR
                     ASVGTPAEFMFRNPGAGDLWITRLEARRDFMQGLKNLSDQGKSFTEFESPLESRILDG
                     NIGYIRIYCHAATLAMPFPVRAFRKSMEKFVQARVTGLIMDLRGNPGGLDELAAAYAG
                     HFTQTPLSFRKVVSFDSGKGGFALNESLNLYVEPRSPCFSAPVCVLINRNTREGGEAL
                     AAALRKMPKVQLVGSTATEGSYGLMDGTITMPGGHVIHYPRGRMLEEDGSVLVTAGTN
                     RVSPVAPDVTVPYTMEKCESLFRKEEDPVLKQAIAILSAGEADSR"
     gene            23312..24004
                     /locus_tag="PKN92_01465"
     CDS             23312..24004
                     /locus_tag="PKN92_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNZ47490.1"
                     /translation="MAIKPSLMTTTLWDFPSQQYGKETQGSKQYKGATPAYIQWNLIQ
                     RYTRPGELVVDPMAGSGTTLDVARELDRRALGYDVNVTRKDIFNCDARKLPLEDEKAA
                     FVFVDPPYSTHINYSDDPRCIGKLDARENAYYEAMDQVIGEIARILRPGRHMALYVSD
                     SLVKGKHFCPIGFELFGLLRKRFTPVDIVCVTRHNTDLSKGNYHKAAQEGNFLLRGFN
                     YLFIMQKPLRPG"
     gene            24133..25434
                     /locus_tag="PKN92_01470"
     CDS             24133..25434
                     /locus_tag="PKN92_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNZ47491.1"
                     /translation="MSTESNSYRPGTLALHAGQEPDPTTGARAVPIYQTSSYVFRDHD
                     HAAKLFALQEFGNIYTRLMNPTTDVFEKRVAALEGGVGALALASGAAAITTAVLNITR
                     AGQNFVSSQTLYGGTYNLFAHTLKDLGIEARFVDASDASNFEKKIDGNTRFLYLETIG
                     NPANDVVDIEAVSAVAHKHGIPLIVDNTVASPLLFRPFDHGADIVVHSATKVLGGHGT
                     SIGGVLVDSGKFDWDNGKFPELTEPNDSYHGMKFYEAFKGIGNISYIIKARVTLLRDM
                     GACLSPFNAFLLLQGMETLHLRVPRHSENALATAQFLEKHPAVAWVNYPGLPSHADYA
                     RAQKYLPKGQGAILGFGIKGGSGAAVKFINSCKLASHLANILDAKTLVIHPATTTHQQ
                     LSEEEQRAAGISPEFVRVSVGLEDIADILEDFDRALTASQQ"
     gene            25622..27445
                     /locus_tag="PKN92_01475"
     CDS             25622..27445
                     /locus_tag="PKN92_01475"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNZ47492.1"
                     /translation="MSTETTTETGSVGLVQTQSLLLEETRDGFTLESGAAIGPVTVAY
                     ECYGTLNEEKNNAILVCHALSGDAHAAGRHRENDPKPGWWDGMIGPGKGIDTNKYFVI
                     CSNFLGGCKGTTGPMSLNPATGKAYNLDFPIFTVGDMVRVQHALIRQLGIDQLLCVLG
                     GSLGGMQALEWATRYPDDTRSVILLATSHYTGAQQIAFDAVGRSAIQADPEFHGGDYN
                     PARGPRKGLAIARMLAHITYLSDQSMDRKFGRTLHRSGSLGYRFGSEFNVETYLDYQG
                     ERFVNRFDANTYLYVTKAMDYFDISNGFESLDASLARAKPRTLAISFTSDWLFPPHMS
                     QDIVYALARNQKEVAYCNIRTDAGHDAFLLELKEISRLISGFLIHTLYPERDCENCPP
                     KPCDGAQDQVPIKNDRLGKLKRVDYEMIVDLVEENSRVLDAGCGEGELLCSLQRRKNI
                     TGVGIELDQEKVIRAIGCGIHVIQANLDKGLTQLPDNSFDYVVLSMTLQVVKNPAFAL
                     KEMLRVGKKCIVTFPNFGHWRVRTNLAFKGKAPVTPNLPYSWHDSPNRHVLTIKDFRQ
                     FCDSLGIGIEQEIALNADGRARLMPNLFAEEAVYVLTKNGF"
     gene            27572..30412
                     /locus_tag="PKN92_01480"
     CDS             27572..30412
                     /locus_tag="PKN92_01480"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNZ47493.1"
                     /translation="MPFKTPVPVTMRSIGWIPLLICFFFCSGASFADTALRQAAWLRD
                     PVFQGVPVLNLFHREKDPAPKAEGPAHVHTLFRREVTLADTPVRAILLITADDYVKFY
                     INGYFAVQGPAPSYHFAHHCLEMDVSSFLKKGRNSLAAHVYYQGLVNRVWNSGDNRSG
                     FAAVLQLTYADGRTDEIVTDEQWRCHSLQAFTTGEIIGYRTQFQENIDMRLMPRGWRT
                     TGFGDLSWTTPLAGGQDHVFIAQPTPPLQVSLREPARTQEGKKGRYLYDFGQTIVGAA
                     QIRLQGKKGHKIIVRHGEELQPDGWVRYEMRANCRYEETCILSGDPDLIEFYDYRSFR
                     YLEILNAPEAPEVRVAVRHYPFQPEAVSFRSENKLLEQIWAICQQGVIMGSQEVFVDC
                     PSREKGQYLGDTLITARSHMWLTADPRLTNQAIRAYVHSACVHPGLMAVAPGSFMQEI
                     AEYSLQFPLLLLHYYQMSGDRDTVEKAVDSVFSGLFDYFAGFENERGLLAGISKETGK
                     WVLVDWPENLRDNYDYEYSLQAGNTVLNAFYYGALRTAAALERILGRNGKTYDQRAEK
                     LAESFARHLVNPATGLYVDAPGSQHSSLHANAVPLFFGLTAGADKDAMLNLIREKKLS
                     CGVYMASFVIEACFKAGAPELGFSLLCSQDEHSWHEMIRHGATTCLEAWAPDQKWNTS
                     WLHPWSSSPIYLLAQYVFGISPAEAGWKRIRFAPAEIPGLPAMSLRLPLPGGRSVSVN
                     YDPEKGYSAMFPPDISVDKNAAPGSKKGIGTEALKAANWQERVGAAPGLWVSIPEQRL
                     YIIESGQVLDTMVCSTALRGMGSVINSQQTPPGWHRITGKIGDGEPVGRVFRSRQATK
                     ELWKPGMTSKEDLVVTRIFILDGLEDGLNRGKDDQGRIVDSRQRFIYIHGTNHEEELG
                     TPGSQGCIRLANDDVVTLFNRIPEGTLVYIEP"
     gene            complement(30442..31095)
                     /gene="deoC"
                     /locus_tag="PKN92_01485"
     CDS             complement(30442..31095)
                     /gene="deoC"
                     /locus_tag="PKN92_01485"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNZ47494.1"
                     /translation="MQVAKVIDHTLLKAFASEEEMKALCEEAVRFSFRTVSVNPVWVA
                     FCKKQLQGSSVGIDACIGFPLGANTAHMKVEETRDAISNGATEIDMVINIGAVKSGYG
                     SYVEREIAAVVKAAGTAPVKVILETSYLTHDEKVEVCRMSVRAGAAYVKTSTGFGTRG
                     ATVDDVRLMRETVGDRLGVKAAGGIRSYGDVMRMLRAGATRIGTSAGVAIVEDAPGS"
     gene            31350..32303
                     /locus_tag="PKN92_01490"
     CDS             31350..32303
                     /locus_tag="PKN92_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47495.1"
                     /translation="MKKIRVGVVGIGHLGYHHARLYRTLEQAELVGVADCSEAARNRA
                     AEDFNVKTVSSVEALLDLGVDAVSVATPTETHCETVLAALAQGAHVLVEKPLASTIEE
                     GEQMVEAAAAQGLLLQVGHVERFNGAVQALFDAGMTPKFIECHRLSPFPGRGSDVSVV
                     LDLMIHDLDIVMKLADSPVISIDAVGVPVFSAQEDIANVRLRFQSGCVANLTSSRVSL
                     ERMRKIRIFDPNAYVSTDYSAQEVLVYRKKPGTLPPDANPMESIEITPLAVKKEEPLR
                     MELASFLEHLQSNTPVAVSGEDGLQALKLSWQILESIRKNG"
     gene            32300..33400
                     /gene="lpxB"
                     /locus_tag="PKN92_01495"
     CDS             32300..33400
                     /gene="lpxB"
                     /locus_tag="PKN92_01495"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNZ47496.1"
                     /translation="MKTIFLIAGESSGDIHGANLIRSIKKKNPEVHCFGLGGQAMAAA
                     GMELIHDLASDAIMGFVEVLRRVRPLRRLFLDTVALVKERRPDVIVLIDYPGFNIRFA
                     EAVKELGIPVVYYICPQVWAWKKKRLDTIARLVRKALVIFPFETDLYRDKGVYCRYVG
                     HPLLDELKDSLHSFPERKDCVIGMLPGSRSQEIRRIAPVMAQICGDILAVRPEARFLA
                     PCTNEARAAEVRAAFSGLPVEIVVRPAREVFLQSRCCLVASGTATLEAALFETPLVIL
                     YRVNRLSYWLARLLVRIRFIGIVNILVGSEIVPEFIQYEATSQKITPVLLELLEDSPL
                     RSQMLANFCMLKRHLGGDGASDRAADEILTLL"
     gene            33441..35096
                     /locus_tag="PKN92_01500"
     CDS             33441..35096
                     /locus_tag="PKN92_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ47497.1"
                     /translation="MLPYFLAALLLFSATSAFSAAERRHDLVIYGGTSAGVIAAVQAV
                     QMGKSAVIVAPERHLGGLTASGLGYTDTGNKAVIGGLSRNFYHRVWQHYSQKENWRWQ
                     DRESFRNRGQGVPAMDGEFRTMWVFEPHVAEDVFEELIREFNIPVFRDEWLDRDTGVI
                     MEGNRITSIRTLSGNIYHGSMFIDAGYEGDLMAAAGVSYTIGRESNSIYGETLNGVQK
                     GNAKYHQFNECVDPYVIPGDPESGLLPRIHNDAPGEDGEGDKRIQAYNYRVCLTTVPE
                     NRIPFPKPDNYDPLQYELLARELDLGFERMLRKFDAIPNLKTDTNNYGSFSTDNIGMN
                     YEYPEASYEKRFEILREHRKYQEGYFWFLSKDPRVPEGMREIMSKWGLAKDEFTDNNH
                     WPHQIYVREARRMVGDFVVSELHLRRTIPTQRSVGMGSYNMDSHNTQRYVDRDGHGRA
                     CARNEGDVQINPGGPYPIDYGAIIPKEKECANLLVPVCVSSSHIAFGSIRMEPVFMIL
                     GQSASTAAVLALDQGISVQQLAYPDLEAQLLADGQVLHYETQN"
     gene            35188..36645
                     /locus_tag="PKN92_01505"
     CDS             35188..36645
                     /locus_tag="PKN92_01505"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated pathogenicity-related family
                     protein"
                     /protein_id="HNZ47498.1"
                     /translation="MRNHSMWKGTGGIVLSALLLALCISGTASANNLRVSENLTPLDE
                     YVAAPDDNYRYELRYSKQEKGFSEHVLYMASQRWLTEELVDRTLWEHWLVLVVPDEPQ
                     CSTALLTIGSGKNGGDMPQGADDTLRRSAKATNTITAKLFQVPNQPLYFTDDGERRTE
                     DAIISHNWDKYLRSGDPIWLTRLPMTKSVVRAMDTIQDFCGGKAGGEHKVDQFVVTGA
                     SKRGWTTWTTAAVDKRVVACIPVVIDLLNVVPSFIHHYEVYGFWAPAVDDYVNSRIFD
                     WIGSPEFDALLKNIDPYSFRERLTIPKLVMNGAADQFFLNDSWQFYWDDLKGPKYLRY
                     APNAGHGMDNADAAGTIIAFYQHIIEGKPLPEYAWSFPDDQSIRVTANTVPKTVKLWQ
                     ATNPEKWDFMLQEGAPVWTETVLEAHADGAWVGRVNSPEKGFTAFLVELTFPGLHDDV
                     LVLTSPTRVTPNTPQHHLEMPTDFSAGFLHRNPAP"
     gene            36781..38178
                     /locus_tag="PKN92_01510"
     CDS             36781..38178
                     /locus_tag="PKN92_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNZ47499.1"
                     /translation="MRIRALSGDKWETTAMLDSFFGITRSGSTLRREVVGGFTTFATM
                     SYIVFVQPTVLAMAGMNLGSVLLATCLSSAAACFLMGILANYPIALAPGMGENFLFVF
                     TICGTMGFAWPSALAIVFISGALFTLLSLFSFREKILDVFPPSLKHAIGPAIGLFIAF
                     TGMQWAGIIVISPATMVTLGPLKHAAPLTALAGIAVTTILLARGLNIAILAGIASTTL
                     LGVLTGVIPATLPDIVFSTESFFNLNFRDLLDHWQNALIAIALFFFLDLFDTVGTLVG
                     VSVQAGFLTEDGKLPRAGRAFLSDALGTCIGALFGTSTVTSFIESATGVAAGARTGLA
                     SLVTGLCFIAAILFAPLVALAGTNIGPDYYALLGLDHAAVAMYPTVAPALIVVGFLMM
                     APLKRINWGDVTESLPAFFTVVVMVFGYGITEGISAGCISFAGIKAATGKHRDVHPIM
                     YAIAAALLLRYIFLK"
     gene            complement(38185..41502)
                     /locus_tag="PKN92_01515"
     CDS             complement(38185..41502)
                     /locus_tag="PKN92_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ47500.1"
                     /translation="MQPSFALHAPFQILAGFVLPLFCLTLLLAGSLCMHACAAEDLKD
                     WQNPELLGINTLAPHATMVICPDADTALQIGEVSNAERVKSPFYRSLNGDWKYKYAKN
                     HTERVANFWEKDFDDSAWTTIPVPANVEMHGYGIPIYVNIPFPWPRPWNPPFVPEDDP
                     NNTVNSYRHRFTVPEEWMGRRALITFDGVNSMFYLWVNGQFVGMGKDSRTPVEFDISS
                     FLKAGENIIAVENFRWCDGSYLEDQDFWRMSGIFRDVYLWSPAQVHIRDFEVSTLLDD
                     AYKDAELTVKAEVENHTAESVPLTVRARLLDANQKQVCTFTENVRASARGDGQKISLS
                     ARISDPLKWTAETPNLYTLLLSLENTDGSVIEVLPSKVGFRRVEIKDGNLLVNGKRIL
                     VKGVNRHESDPDRGQVMTRESMIKDILLMKQFNVNAVRTSHYPDVPAWYNLCDEYGLY
                     VVDEANIESHGMGYGKESLANPPVWMKAHMDRTVRMLERDKNHPSIIIWSLGNEAGNG
                     PNFMATYDWLKERDPGRPVQYERAGFHRNTDIYCPMYSSPAALRDYADGKKVDGGWGR
                     EFLLKEGEPRTRPFILCEYLHSMGNSTGNAWDYWNLIYNKPYLQGGFIWDWADQALRE
                     STAQKPPRTYKGAKEGEDFFWSYGGDYGPEGVPSDKNFNCNGVITPDREPHPGLYQVK
                     HVYQYIHSKLVDGAARQVEVKNWYDFVNLKDWLELHWQVSADGAVIQEGTQAAPSLEP
                     QNTTTLTIPVADFEPQGGVEYFLTLSFRLAQDELWAKKGHEVAWDQFLLPDAAPAPVL
                     AAPEGQAVTVSDEAEQLIVATGDLALRFDKKTGALVSMTSGGLELLHSPLRPDFWRAP
                     TDNDRGRAIDKSQGIWKTAHAGAVLDSLSVKESSPKRVVVEQQLSMKEADVRWFSRFT
                     VFNNGDIRVENDFVPRNTKQQKLPRLGVQMVLNPGFDRITWLGRGPHETYIDRKDALV
                     GRYSGTVRDQFYKHYANPGESGNKEDVRWAALQNAEGAGLLIVADKAHLLGVNAMHHT
                     VEDLAAAMHPFELPERDEIVLNVDWRQQGVGGNDSWGAWPEEEYLIICRPQTFRFWMR
                     PLQAGADPESLARRSRP"
     gene            42108..43976
                     /locus_tag="PKN92_01520"
     CDS             42108..43976
                     /locus_tag="PKN92_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNZ47501.1"
                     /translation="MKRISLLSFVLCLLSVPCPLSAAAREPAPSILLDESYGALLESS
                     SDTLALWWASSGWKVSRERGLPEEKASSISLELARNEWEATQLVLRPHKAMSGLHLSA
                     TDLQSASGARIPADCIELLRVRYLAVEKASDGFGCEGFWPDPLPPLENSFDLEAEINQ
                     PIWIRVYVPGGSEAGKYEGALLLEGEGTSVRIPLQLRVYGFTLPDRSSCTSAFGFSPA
                     WVYQYHGLDKQEDRKAVLDLYWESFRKHRISPYTPAPEVAPEVNWVPLERDACQHVSD
                     EDAQLLTEHPLTPVFDWSAWDQEMDRVFKTFHFNSFRFGVPGIAAESYQGFPEGSRGY
                     DLAFNAWGAAVESHLRETGRLNDAYIYWVDEPTENDYPQVMKGFNQLKKALPGMRRML
                     TEQVEPELAGGPDIWCPLLSLYNHERAEERRAAGDSFWWYICTGPKQPFLGLFIDHPG
                     TDLRAWLWLTWKYGIEGILIWHTNWWTSNTAYPDQMQNPYKDPMSWQTGYGAKTGDKK
                     PWGNGDGRFLYPPERAADGQPEQPVLEGPVDSIRWELLRDGVEDYEYFVILRRLLAQK
                     SDNLDEKERQLFNELLLVPEKVASGPKEYTKDPAPLERQRHRLGRAIERLQALESK"
     gene            44070..44933
                     /locus_tag="PKN92_01525"
     CDS             44070..44933
                     /locus_tag="PKN92_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ47502.1"
                     /translation="MSAMRVFTYDSLEERRPRHILMDLIRARQLLLDLIWRDLRVRYR
                     YAVMGFLWAVLEPLAFTVILTLVFTLVFAPRVPDAAQTAGPPYVVMLLCGIIFWQYFS
                     TSVTTATTSVVANQNLVKKVRFTREIIPMAACCVPLVQLGIGLTLLFIVHLFLGGALS
                     SALIWLPFVFAIQFVLTLGLALLFSCVHVHFRDVSNVVTVGLLLGFYASPIFYPLEMV
                     RESRLPQWLQQLYLLNPMAGLLTAYRQILLEYRFPDWQLLIAPSVIAVSAFIAGLLLF
                     RRIAATMSDYL"
     gene            44971..>46101
                     /locus_tag="PKN92_01530"
     CDS             44971..>46101
                     /locus_tag="PKN92_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ47503.1"
                     /translation="MVRIEKTKKRVYTRRGVVWLGQTCNQRCYFCYFISRIEDHCHPL
                     HAFMELDKAKEIMNRLRYFYGNRSVDIQGGEPTIFKEILPLISHCRDIGLYPTLITNG
                     LQLAKPGRAESFREAGIRDFLVSLHGYGKQHDEVVGVPGAFEKISQALDNIREADIPI
                     RINSTMSKPVIPVITQVAQKAIDAGALVVNYIAFNPFEDQHTGRRRHDTVARYSEIMP
                     RLTEAIDMLEEAGVEVNVRYLPMCLAEKRHRKNFFNYQQLSFDLHEWDYQSWLWTMMM
                     PQMMKEGGLIPPFRLGTGARRLYKADPLYLRDNAAKHPVKTGFKYAVQRAVAKVQQTI
                     NKDGIYREEAKQRATVDCGYRQVPACASCHLRHICDGFHGDYT"
BASE COUNT        10340 a        12623 c        12300 g        10838 t
ORIGIN      
        1 aaactgaacg cagaaagaac ctgctcatgg aaagtgatat ccggcatctt cctgaaacag
       61 actgcaggaa ggccgctaaa gcacttgccc gttccttccg gaacgacccg ggctgtctct
      121 ttgttttttc cacgccggaa cgctgcgaaa aagggatgcg ccggattttt cggagctggc
      181 tccgtattat ggccacgcat aaagcctgct ggaccgtacc ggactgttca ggcgttgccg
      241 tgtggtacag ctccgcggcc aaacctgccc ttgcttgtgc tgcacttttc aaaacaggcc
      301 tctggcgcct gcctctttac atgactccgg cggagcaata cagaggcatc cgtttttttc
      361 gggatctgaa aaagcgtaca gaaaatgaag aaacagaaga gcattgggtg ctggatacac
      421 tgggcgttgc accggaagcc cagggaaaag gcatcgcctc agcgctggtc aggcagggac
      481 tgcagcacgc cgacaaggcg ggcaagcccg cacgggttat cacgcacaat cccgcaaatg
      541 ttccttttta ccggcagttc ggttttgagc ctgttgaaag cggctgcatc gccggaacgg
      601 aagtccaggt catcgtgatg cgccgcaaag tttccgacac tgtatgctgt tgattccttc
      661 cgtttcttcg ctattcgaaa gcgctcctga gattctgcta aaatacgggc atgaacctac
      721 ccaatacatt aactgttgcc cgctgcatcc ttgcaggcat ttttgttgtg ctcatgtctt
      781 ttgatcacaa cctgtgttac ttcatcgcct atcttgtttt tatacttgcg gcactgaccg
      841 attactttga cggtaaaatt gcacgggagc agaacctgat caccaatttc ggcaagctgc
      901 tcgatcccgt tgccgataag atattaatgg ttgccggttt tatcatgctg atgcgtgtgc
      961 cggatctgac cattcccgcc tggactgttg tggcaattct tgctcgtgaa tacctgatca
     1021 cgggtgcgcg gacacttgcg gcatcagaag gggcgatcct gcccgccaat gtatggggca
     1081 aggcgaaaac agtcatccag atggcttatg tttttgttgt gctggcactg gtcattctgc
     1141 tgcgctttgt cttttctgtt ccggcactgc gcgatgccat cccttacaca agggaaatgt
     1201 gcgcacaaac cctgcattat ctttcttttg gcggcattat tcttgtttca atctacaccg
     1261 ttgtttcagg cattcagttt acctgggcaa actggaacgc tctgaaactg cacgatattt
     1321 aaaacggggt ttcgtttatg atatcacgtc acccatcccg tgccattgtt gatttaaacg
     1381 cctatgcctc caatctcacg ctggtgcagc gtttcgccgg gaaagagatt gcgctgatcc
     1441 cgattctcaa ggcgaatgcg tacgggcatg gcgcaatacc gctggcacga aaagcgctgg
     1501 aagcaaaccc gctgcttttg ggagttgcaa cggtcgaaga aggaattcag ctgcgcgaag
     1561 cgggcattga agcccccatc ctgttgatgc ttcagcccaa ccaggaagcc cttcctttgt
     1621 gcatccagta cagactgaca ctgatgatct ccgacacagg gctcgctgaa acactgggac
     1681 ggcttgctgc gcagacgaat accgtagtgc cggtgcacat caaagttgat acaggcatgg
     1741 gacggcaagg tgtttccctc gccgatgctt tacagacaat ccagtatatt tcccgcattg
     1801 tccatgtgga tattcagggt atctgcaccc atatgtccac ttcagaaatc caggacgacg
     1861 gatttaccca tggacagatc aaaagtttta aggcgctgct caagcaggtc gacaaggcgg
     1921 gggttcccta tgaagtgacc catgccgcca acagcgccgc tattgttaat taccgtaaaa
     1981 gcattctgga cgccgtacgc cccggcctga ttacttacgg tgtatggcct tcccgggaaa
     2041 aaaatgccac accgcaatta aagccggtac ttcgctggga aacaagaatc gtttatttgc
     2101 gcgatctggc gccgggatcc accatcggct acggcaggac ttatacgacc ttcggcaata
     2161 cacgcacggc gattcttcct gtgggctacg cagatggatt ccgccattcc ctgtcgaaca
     2221 aggcagacgt actgatccgg ggcagacgct gcccggtacg cggcgctgta tccatggatc
     2281 agatagtcgt agacgtgtcg gcactgccca atgtggcggt tggggatgtg gcaacactga
     2341 tcggcaaaga cggcactcag gaaattaccg cagaggagat ggcggcaaaa gccggaacga
     2401 ttccctacga tattctgacg ggcatcggca cccgggtgca tcgggaatac ctgcatgaga
     2461 caaaaacaga ccattgaggg gcgtttttac cgcgcaaaca aacctgttgt acacatcact
     2521 ttgttcacta ccggttaatc ggggcagaat ccatgctgga tcactatttc atcgatgcct
     2581 acaatgtcat tcataagtct tcttatttgc gccagcaggc gcaaagagat ctggaattgg
     2641 cacgggaaac gctgctggaa atgtccttcg ccttcagtct ggtcggtcaa aacgaaatca
     2701 cggtggtttt tgacggaaaa gaactgcagt atgacattcc gattctccac cggctcggca
     2761 atgcgcccaa tctgcacatc tttttttcac cgcacggcac aacagccgac acggtgattg
     2821 agcgcaagat ctatcaggcg cagaacagaa tgcgctgcat cgtggtcagc aacgaccgct
     2881 cactccgtga gcaatgccgt ggcatgggtg ccatgaccat ggaagcagac agttttatct
     2941 ccaccataaa acaaatgcag cagcgtacac aggaacagct tagcagccgt gttaaaagcc
     3001 gtgcgaccct tattgaagac agattgaaca gccgcagcct ggaagctctg gctgccatca
     3061 gagaagaaat tgtgtcagcc ggcgacaaaa gaaaaaagag gtgaaaacgc tgtccggaac
     3121 aaatacgccg tcagtatggc gaaaacgagt tttttcagtg ctgcaccttg tctgtgttta
     3181 gtgccaggca gcccatcccc attttccgct ttcatctgaa acacgtcagg gaggattaca
     3241 gatgactctt cacatcatcg gcggcagtgc caaaggaagg cggctctttt ctcctcccga
     3301 tcacaacacg cgcccaactg ccgctagagt gcgcgagagc cttttcaaca tcctgcagga
     3361 catcatcgaa ggatcacact tccttgacct cttctgcggc gccggggcgg ttggaattga
     3421 agcgttgagc cggggtgccc tccacgccac ctttgtcgac cagcaggcgg atgcgctgga
     3481 tctggttcgc cggaaccttc ggctatgcaa aatggaggga caggcggatc ttgcgcgtct
     3541 gcaactgccg ggaaagctga gccgtctgac cacgccgtac catattgttt ttgcggaccc
     3601 tccctatgcc ctgccctgcc aggagcagct gctgcgcgcc ctatgcaccc atcgtctgct
     3661 ctgcccggac gccatcgttg tggtggaaag tggcaaaaaa gccgtgctgc cggactctgt
     3721 tgaatctctc gccagaaaag acacccgtac ttacggcgac agtgttttgc acttttactc
     3781 ttgacacagg atatagtatc tgttatccta atatagaaga agtgtatacc atatcttgca
     3841 tttaccctga ttattttatt cgggtgtaaa agcagcaaca gagaaagtca taactatgcg
     3901 gtgtcccttt tgcggctttg acgacagcaa agttgtggat tcgcgtgtgt ctaaagaagg
     3961 cgattcaacc cgtcggcgac gcgaatgcat cagctgtacc aagcgcttta ccacttacga
     4021 gcggattgag gaagtggcgc agatggtgat caaaaaagat ggacgccggg aaaactttga
     4081 ccgctggaaa ctgaaaagcg gcatcatgaa ggcaatagaa aaacggcccg tcagtcttga
     4141 gcaggtggat gcactgatta atgatgtgga gcgggagctt tacaacggtg gcgagcatga
     4201 gattttcacc cgggatctgg gcgaggcggt gatgaccaaa ctgcgctatc tggacgaagt
     4261 cgcttatgtc cgttttgcgt ctgtttaccg ccagttccgt gatgtgaatg aattcatgct
     4321 tgaacttcag gatatgattc cttttgcgcc gccgggcaaa acgggcaaaa aagcctgatc
     4381 ctgagtaaac agtagcagca aacgctgtat cgtgttatac tgtatgcata agctgccatg
     4441 ctgtttgacc gcagacgata gaacaggagg aaaaaatggc gtacgataaa acgattctcg
     4501 tcgtggatga tgagcccgat gtggtatcac tcctggaagc gactcttaag ggagacggct
     4561 tcaacgtact gactgcctac gacggtattg cggcacttga tctgtgcagc acggaaaaac
     4621 cggatctggt actgctcgat attatgatgc ccatgatgag cggttacgaa gtgtgcgagc
     4681 agatcaaagc aaatcccatg acccagcaga ttccggtgct gtgcatttca tccgcccata
     4741 cgccggaagc ccgtgcgcag tccatccggg tcggcgcagc ggaactgatc aaaaagccgt
     4801 tcctgccgaa agaactgatt gcacagatcc gaagacatct gcccaaagaa aatgatttct
     4861 gatggctctg tatcgccccc gtgcttcctt ttttcccggc gttttttaat caagtcgaag
     4921 cgggcgggaa aggcaaaatg ctcaggatac cccgctgcac tgaagcttca tctgttgccc
     4981 ggaatagagt gagtttctct tctgctttct gtttcttttt tgagctgtcg cgcggggctg
     5041 cggcttttct ctctcatccg taatgccaaa tcaggacttc tttttacatg aaagcccgaa
     5101 gaaaaaaaac gcttcctctt gtcgccatag tcggcagacc gaatgttgga aaatccaccc
     5161 tctttaacag gattatcggg aagcagcgtg ccattgttca tgatatagag gggattaccc
     5221 gggatcgttt tatcaatgaa gctgtctgga aggactgccc ttttcgactg gtagatacag
     5281 gcggactcgt tgaaaatccc atcgacgaaa ttacccggaa gatgcaggaa caggtctttc
     5341 acgccattga ggaagcccgt gccattcttt ttgtgttgga cgggcagcag gaaattaccc
     5401 gtgccgatga gcaggtggcg gagctgctgc aaaagaccgg caagcctgtt gtgcttgcag
     5461 tcaacaaact cgacaaccat cagctggcta tgaaccagta cgaatactac cgcctgggat
     5521 tgggtgatcc tttccccatc tcttcagggc ataatctggg tatcgaagct cttatggatg
     5581 ctcttgttac ccatctgccc gctccgcctg cggaagaagc gaaagcagcc cctgaagatg
     5641 gggacgatga taatgaaacg acagacgaca cagaagaaac tgatacagcc gatgaacctc
     5701 agccgatcaa agttgccatt atcggcaggc cgaacgtagg caaatcttct tttatcaatg
     5761 cgcttctgaa tgaagagcgg actatcgtta cagatatccc cggcaccaca cgggatgcca
     5821 ttgatattga attccactgg cacgggaaag actacattct tatcgacacg gcagggctcc
     5881 ggaaaaaagc aggcatccgc cagcatgctg agcacttcag cgttgcccgc tcccttcgtg
     5941 ctatccgccg ggcagacgtg tgtctggtca tgatggaagc aaccgaaggc atgtcggagc
     6001 aggacaagcg cattatcggt tatgctctgg agcagggtac cgcttttatc ctggtctgga
     6061 caaaatggga cttgatagaa aacaaggaac ggcgttttaa agcactggaa gacgatcttg
     6121 atcttaaaat gccgcaaatc cagtacgcgc cccgcctcac catttccaac attacccgca
     6181 aacggctctt ctctgccttt gaaatcgtcg atcgggtcgc aggagcagca aaaaaacgtg
     6241 tcggtacagg cgagcttaac cggctgattg aagaaatcaa aacaaagcac aatccgccca
     6301 gccagaaagg aaagcacgcc aagatccttt acgccacgca ggcaagtgtt cagccgacga
     6361 cctttgttat cttcgtcaat cagaaacgtc tttttcattt cagttacctt cggttcattg
     6421 aaaatcagat tcgggagcgg tgggcttttg aaggagtacc cctccggctg gaattgcgcg
     6481 aggaaaaacg gaaggagaga aaaccacgat gaccatcccc cttgccatcc tgctttctta
     6541 tcttgccgga tccattccga cgggactctg gctcggtctg cgttttcggg gcattgacat
     6601 tcgtaaggaa gggagcggca atatcggcgc cacgaacacg ctccgcgtac tgggtaagcc
     6661 tttcggcgcc gttgcactgg ctgtagacgt attcaaaggg tgggtggctg tcactgtttt
     6721 ttcgctgctc aatccctggc tctatttgcc gatcatctgc gggctggcag cgattttagg
     6781 gcatactttc tcgctctttg tccgtttccg cggcgggaaa ggcgttgcca caagtgccgg
     6841 tgcgtttctc ggactcgcac ccatgcccat gctgatcgcc ggtgcagtct ttctggtcat
     6901 tgtcggaatt acccgcatgg tcagtctggg atcaatcact gccgcggcag tagcagccgt
     6961 gtccgttttt ttctttcctg tatccggtcc ggtccgaatt gttgccgtac tggtcgcagc
     7021 gctgatcatt ctccggcatc attcgaatat caagcgcatt ctgaagcgcg aagagccccg
     7081 gctgtgatgc atgccgggca gtacagcctc catgcagcag gttgatgcca tgctctcttc
     7141 tcccgacccg gacagtaaac ctttttttac cgccgccgat cttgtcattg cctttgtcgg
     7201 cggtcttgcc gctttaaccc tctatctcct taccctggcg ccgaccatta gcggagagga
     7261 cagcggcgag tttattacgg cagccgccac gctgggcatt gcccatccgc ccggctatcc
     7321 gctgtactgc cttctggggc acctcttcag ctggatgcct ttaggcactc ctgcctggcg
     7381 catcaatttc atgtcggctt ttttctccgc tgcttccatt tctcttgcca tactcaccgt
     7441 catctatctg acacgcaacc gacgggctgc cctcatggca gggctgatgc tcgcctgcac
     7501 acgggatctg tggtctcaat cggttgttgc agaggtatat acgctgaccc tttttgttct
     7561 ggcagcgtct tttcttaccc ttctgctctg gagcgaccga ggtgaaagcc ggtatctgct
     7621 tttttttgct gcgctttgcg gaaccggcat aaccgtacac aatacgatgc ttctcatcct
     7681 gcctcctgct gcgttatttt ttctgcattc ggaatacaaa gcgaaagaac gcacttctcc
     7741 ggctctgctt ttttacgcct gctgctgcgc tgctctgccg ttcctcctgt atctgtatct
     7801 gccccttcgt tcccgcgcca atccccttct ggattggggc aacccggaaa cactcccgct
     7861 gatgctgcgt cacattttcc ggagtcagta tgccttcatg gtcgaccagt atccgagaga
     7921 tttggggcgt tttctggaac agctccgggt ttttctgcgc tttgcccggg aacaggccgg
     7981 gctgatcccg gctgtgtgcg gtctgacggg attgctgctg ctgcttcgcc aacggaaaga
     8041 ctatgcgctg tttcttcttt tttcgcttct gctgattccg gcggcctttt cgttctggca
     8101 gaactttgaa ataaccagag aatggctctg ggtcatgcgt gttttcggga ttccttcctg
     8161 gtacatcctc gctctgggct gcggtatttt tctgcatcgg ctttctgaaa cttcgcaaaa
     8221 gatgaagccg tttttttggc tgtttgccgt ttcttttatc cttttccctc ttgtcagaaa
     8281 ctatcgatat aatgacaaat cggattatta ctgggtggaa gattatgcaa ggaatctggc
     8341 agatcatctg gagccgggtg ctcttttcat atccgaatcg gatcatgcca gtttcagcct
     8401 gctctatttt caggctgtgc tcggcgaacg aagagatgtg gaaaatatcc ggcgctacgg
     8461 atatttacag acacctcttt ttgacgggct gccgaaggaa ctgaaagaaa agaccggtgc
     8521 tttcccgccg cggcgatacg atgccgagct gatcgcctgg ctgctcgatc atacaacgcg
     8581 ccctctttat gtggcacgtc cgcgcccgct tccctcacag ctcttcgccc gttatgaggc
     8641 atgcggcccg gtatttcgtg cgctgcgtca cggagaaaac ttcagcctgc cggacacaat
     8701 ccgcaccgac aggcgtaaca ggagcgacga tacacggggc gactacaccg ccgatgccat
     8761 tctgtatgag gaatcgatga accgtgcact gcgcctcttt ctttccattc gacaggacgg
     8821 cacaaatcct gaatcggtca aagcagaagt gcttgaagca gtggaaaaag ggctttatca
     8881 ttacacgcgg gacgccgccg ctctgaataa tgtcggcacc ctcgtcgccc gatgcggcta
     8941 tcaggcggag gcgcttgcct acttcgaaga agcatcccgt ttgctgccgc attcccccga
     9001 aattcggaag aacctgcagc gtatgaaagc aacgggagat ctccccgccc gttaacagtg
     9061 cgccttttta ttgcctgcgc atccagtgcc agtaactgac ggtagtctgg ggacgggcgt
     9121 tttgggcggc aacaggctgg agcgtcgccg tacaccgatc cgggaatccg tcatggatat
     9181 aggacagggt cacttccggt gcctgcacag ctcccggcag atcgctgtgc atagaaacat
     9241 gtttccggag ttcctcctgg tctttcggga gcagcagaat gtttttggaa tacggctcac
     9301 catgaaacat agggttacgg tgcgcatcca ctccgagcag ccagggcccg aaaaaaacag
     9361 cccgttcttc cgcaccgttt tcaggcagcc cgtccaaaag atgcacctgt ccctgacggt
     9421 cagcaaaacg cagtgcacac tcaataccga ccgttacccg ttctcctgcc tgccagacac
     9481 gctccagatc cagtgagctg atgctccggc tgatcagctg cgaagccgct tccgccgaag
     9541 gctgttgtga aattgtattg cctactgtcc tgccgtccag gctgactgtg gcggaaagat
     9601 ccttcgccca gccgggcaca cgcaaggaga gaacccgtgt atcttgcggc gcatcgatca
     9661 cttcgatctg aaaagcaagc atatatggtt tttccggcag aatgacccgc tccactttcg
     9721 cccggaaatc cccatcctcg caagctgtct cctgccagag agcaatataa agcccctttt
     9781 ttccgtctct ttccacaggc gtaatatcgt agtcgtgcaa atcctgcaga gcgcgcagtc
     9841 cgtgcatcgt acagcaccac caggcacgcc cggcaccggg atgaggaata tacccctggt
     9901 tattgaagaa gcggtgcccg aagtcgcctg tttcaaactg gtttgcgtag aaaatattat
     9961 tgaggcactg ttccgcccgg gcaagatact gctctttgcc cgtggcacgc cagagctgaa
    10021 ggcacaagcg caaaaaatct gcttccgtac atccttcgtc ccggtcgtag tcttctttaa
    10081 agaattcaag gagcccgcct gtaatcagga tggagccact tgcttccata tcatcaaagc
    10141 gcgcttccac tgctgacaga agagcagggt caccggtgct ttcgtagatc atcatatgcc
    10201 cgcgcaaagt ggtcagatac ccatgggtat gctgcacccc ccgcggttcc agaagagcct
    10261 ccattttttt cgccgagtca cggtagtgct catttcctgt cgcccgtgca agaagctcca
    10321 gtccttcatt gaactgcgta aagcagatga aaccgttcgc cgcctttcca cgcacccgag
    10381 ccattacctc ttccgtggca cattcatcaa agactttgag cagaaaatcg ccaagtttca
    10441 ccgccgcctc aatcactgct ttctcttctg gaaatcgctg ggcgtactcg agcagcccaa
    10501 ccagcagacg gccgttgccc caaagcagcg ccatctgatc cggaccgatg tccgcagcat
    10561 caaacgacag ggatgcatta ccgaaacgac cgtcttcccg ctgacagggc agtatctttt
    10621 ccatcagctg ctgcagccgt gcatcatctt cttctccccg cgatgccatt gccactgcac
    10681 cgataaaacg gcctgatatg tcaccgctga attcatcaaa gcgccgcgga tattccggat
    10741 ccagttttac atccgcaaga ataaaatcgt cggtaaatgc cggctcctga acttcatcga
    10801 agcgctgaaa aacacgctca atgctgtcag cgacccagtc ggaggagctt tgagcagcag
    10861 caccggacgc cagcgaaagg cagtacagaa cagaaaatgc tgtcaaaaag aaacgcatat
    10921 ttttttccct tcagttacaa tttttgttga atctgtgaag agcactataa caactttcca
    10981 gatgcgttgc aacagccgcg gagatgctgc cgcaaagacc tgctatccgg tgtatttcca
    11041 caaccgcaag gtttcgagca gttcccggtt gccctgctgc aaactttttt ccggaaaaga
    11101 aatacgctgc tgcagcacag taaaggcttc ttttgccagc agatccgctt ttccatgatc
    11161 acggaaataa tgaagccact ggatcgtccg aagtccatcg aaccaactgt gaaaatgccg
    11221 gtgcagctgc ctgccccgtc cgtgctggcg gtaaattttt tcccagtgac gaggaaattc
    11281 tttggcggtg agaaactgca tgagcagcgg cgacagttct tcactgcgaa gcagaagtct
    11341 ttccgggctc aacgctgcgt tgtccccggg aagagcaagc cactctttca gaatctgata
    11401 tgcctcgggg tgatgcagat atcggcgggc tgctccgctt ttcaggtgag gacccgtgcc
    11461 gaaaggagtg cggctggaga gtcgccccga cgggaaaatg gttgcgtgcg gtatttgttc
    11521 cagcgtaccc gttttccgca gctgctgcat gaaataaaaa tcttctccgg cgcagtggcg
    11581 gttcatccca ccggcagcgg cataagcatc agcggtgcag ctgataatag agcccagcgc
    11641 aggccaggca taaggcgaac ccgcccagcg aaggtgcagc tcatggtagc gcatatacaa
    11701 ttcataggcg atcatggctt cctgcacttg ccctgaacct tcgagcctgt gggcgtaagc
    11761 ggcatatcct ccgtggcgct tcgcactagc ataataggaa tggagcgcat ccagataacc
    11821 gtcaagggca tgtgaatcag catcaaggtt tacgagataa cgaggggctc cgaaggcaca
    11881 atcatttcgg gacagaagcg caagcccatg atcgagtccg atctttcgtg ccagcccgac
    11941 gccctccccg gcaggcaggc actgaccggg cgaagatgca tcaatccagt tcaatgtcaa
    12001 aggggcacaa taaccatcct gtgcccactg ttccagctgc agcagtgttt tccggttgtc
    12061 ttccctgtcg gacggatcag aatgattttc atccctgtta ttgactacac agattacgag
    12121 gctccggcac agcgtttccg tggaacagtg cgcgagagca gcaagcgtgg ttccaagatg
    12181 ctgcgattcc gcaagtgcgg ggatcactat gacagcggaa acgtggcctg gcggcgatgc
    12241 acccagcagt ttaaattgcg aatgaacgga acgtgtgcga aggtatttct gaactgtgcg
    12301 ggtcactgct acggctgcct gacggcatac ttgatggcgt taagcgccgt gccgagttcc
    12361 gcatcaggaa gccaatgcag gacatgtcct ttacgctcca tgccgcgaaa ccaggtgcgc
    12421 tgccgtttcg caaaacgggc gatggcactg ttgagtttct gaaaaaggtc attgggactg
    12481 aggatggcac cctgcagata ttgcgccaca tacctgtatt ccagacccag cagttcaagc
    12541 cgctcccagc tgagcccgga ttcgtgcagc tgcgccactt cttcaatcat tccctgatcc
    12601 agacgctcct tcagccgtcg ggcaatacgc tcccgcagca cttccttgtc atgatcgata
    12661 accataataa atgcgtttat cttcggcagg ggcggtgccg gatgagaggc tgtgtattcg
    12721 gcaatttcaa tagcgcgaat gagtcgttcc cggtcttccg tatcagtctg gttgtgcaga
    12781 ttctttccgg tgctctccag ccgtgcaatc agctgcggca tttccagatc tgcgagcgcg
    12841 gcacgaagcg ttttgtccgg cggcacttcc accatggcat acccggaaag aaccgcatca
    12901 agataaagac cggtgccgcc aacaatcacc ggcagcttgt ttcgctcctg caccgatgcc
    12961 agcgccggat aaaaagcctg ctgataatga aagaggctga actcatcgga aggatcggcg
    13021 atatcaataa gatggtaggg aatggaacgc ccgttgaaag aatattcgtt cagatctttg
    13081 ccggatccga tgtccagccc gcggtaaacc tgacgcgaat cggcagacag aatttcaccg
    13141 tcaagcagag ctgccagttg tacgccgagc cctgtctttc cggaagccgt cggtccggca
    13201 ataaccataa cactttgcct gttctcatgc ataggcatat aatactgttt cgcagaataa
    13261 actacaattt catacccccg ggacagggta tataaaggaa cctctacgct cctgttccag
    13321 cgccggatca cttcccggag attttcaaaa atgttttttt caagaagact tcaacgctgc
    13381 ctcttttata ttctcctgcc ttttctcagc gtctttcaaa gccaggcgga agaagagcgt
    13441 ttctttgccg actatttcta tctggcaccg gaaacagcaa cagataaatc tgcgcaggat
    13501 cctgcctatg cccggcgcta tcccatgccg gagaggcagg tgcggcggta tgttgccgct
    13561 gacaaaggca gactccattt ttccgagttc agacatgatt actttatgga agaaaagcgc
    13621 gccgacgtcg agcccaccca aattgctctg ggtaaatctg tgaacgaaaa agcaccggat
    13681 ttctttgcac aggttccctc cgaaaagggc atgcacatat ttctggcaga actgtcttcg
    13741 gaagatgcgg ccgctttgcg ccttcgtgtg aatttatgtg cgctgcagcc cggcgaagaa
    13801 ctctggctgc tggacagccg gggcgatgcc gcattcggtc cattcacgca aaggcatgcg
    13861 cgcccggaag gcaactggct gcccactacc gccggcgaca gggcggtgct gctactgcgc
    13921 acccccagga aagagtgccc ccttctggag attcagtctt actcccattt cttcaaatcc
    13981 atttttaatc cttctctgcg tgatcccaaa ccctgccatc tggacgcatc agctgcggaa
    14041 gatcctcgcg cacaggaaat ttcaacagct gtcgggatcc tgatcattcc ccggggaaat
    14101 acgcaaagtt tctgtaccgg tacgctgatc aacagtctgg ggcacgatga cgcaacgctt
    14161 cccgaacctt atctgatcag tgcaggtcac tgttttgaag gcggccccga tgttgcgggc
    14221 atggaagtct tctgggatta ccgcagtgaa acggatgatc ccgccacact gccacgaagc
    14281 agcggtgctt ccctcgttca tgtcaatcat gtccttgacg gtgccctgct ccggctggaa
    14341 ggcgaagtgc cctcaggtcc attcggtcgc gcctgggcag gctgggacag cctccgccct
    14401 gtccgccaga gcctcgttca gtgttttcat tatcctgtcg cctccagcat gaaactctcc
    14461 cggggactca tcagccttgc aaaaaccgat gtatgcaaaa acattctatg cacttcttcc
    14521 tttgaacgcc agataaaaat caactggtat gaaggcattg ccgaggcagg ctcttccggc
    14581 tctcctctgc tgctccctga ttttaacttc cggatcatcg gcatgctgag caacggcaat
    14641 gatcacagct gccgctcgtc tcagaacaac ttcgaccatt tcgccgcttt tcacgaatat
    14701 tttcctgaaa tcaaatgcca cctcgttccc ggcgaaaagt gcgccgaccc tgtgggccgc
    14761 accgcaagcg gctgtttcct tcgccgcctg ttctgtctga acagcgaaac attggcggat
    14821 ctctaccgtt tccgctcggg catccttgaa aaaagcagct ggggcagaag ctgtattgat
    14881 gaatactaca gcctcgcgcc tgtgctcgaa aattggttta atctggatcc tctggcaaag
    14941 gaggctttcg gtcttgttgt ggaagctgcc gctttctggg gacacctcct tcaggactga
    15001 gcctgtaaaa gacttttacc cggcggcata aaaaagctgc ctgccccgaa ggacaggcag
    15061 caataaacag tctcagaatc agtatacggc ttagtacatg ccgcccatat cacctcccgg
    15121 agggcctgca ggcgccttct cgggctcggg cagatcggca atcagcactt ccgtggtcag
    15181 cagcagaccg gcaatacttg cggcattctg cagagcggtg cgggtcacct tggcaggatc
    15241 gagcacgccc gctttgaaca ggtcttcgta ttcaccggta gcggcgttat aaccgacatt
    15301 gccttttttc gcacggacgt tctgtaccac agtggcacct tcgtcaccgg cgttaatggc
    15361 aagctggcgc atgggctctt cgagggcgcg gcggataatg gcggcaccca ctttctcatc
    15421 gccttccagc gcaagcccgt cgatggcatc ctgacagcga aggagggcaa caccgcctcc
    15481 cgcaacgatg ccttcttcca cagccgcacg ggtcgcatga agcgcgtctt ccacacgggc
    15541 tttcttttct ttcatttcga tttcagtggc agcaccgacg ttaatcacgg caacaccgcc
    15601 ggcgagcttc gccagacgtt cctgcagttt ctcacggtcg taatcgctgc tcgtctcttc
    15661 aatctgttta cggatcaggt tgatgcggcc cacaatgtcg gcgctcgcac cggcaccttc
    15721 gacgatggtg gtcgtgtctt tgtcgacaac aacacgtttc gcacggccga ggtcagccat
    15781 ctcaacgctt tccagagaac ggccgagatc ttcggtaatg cagagaccgc cggtcagaat
    15841 ggcgatgtct tccagcatgg ctttgcggcg gtcgccgaag ccgggggcct tcactgccac
    15901 tgcctggaaa gtgccgcgaa tcttgttgac aaccagcgtt gccagtgctt cgccttccac
    15961 atcttcagca atgataagca ggggtttgtt gctcttggca acgttctcaa gcaggggaag
    16021 cagatccttg agggaggaga ttttcttctc gtaaatcagg atgtaggctt cttcaagcac
    16081 agccgacata ttttcggaat ctgtcacgaa gtagggggac agatatcctt tgtcgaactg
    16141 catgccttca acgacatcca gcgtggtctc gatgctcttg gcttcttcca cggtgatggt
    16201 gccgtcgttg ccgactttgt ccatggcatc ggcaatgata ttgccgattt ctgcatcact
    16261 attggcgctg atggaggcaa cgttgcggat cacgtctttg tcgtcgcgca cctgtttgct
    16321 catacccgca agtttttcca caacagcact gacagccgta tcaataccgc gtttcaggga
    16381 catggggttc gcaccggcag tcacattgcg caggccttca cggaaaatcg cttcggcaag
    16441 cactgtggcg gtcgtggtgc cgtcaccggc gacatcggaa gtcttggacg ccacttcctt
    16501 caccatctgg gcacccatgt tctcgtattt atcttccagt tcgatctctt tcgcaacgct
    16561 cacgccgtct ttggtcactg tgggggcgcc ccatttcttg tcgagaacaa cgttccggcc
    16621 tttgggtccc agggtcacgc gaacagcacg gctcagctgg gtcacaccgt ccaatacctt
    16681 gcggcgtgca ccttctccga attccaattg cttaggcata ctcttgattc tccaatgttc
    16741 aggcaggagg cctgcttatt tctcgatgac tgcaagaaca tcatcttcac gcatgatgat
    16801 gtgctctaca ccgtcgatct tcacttccgt accggcatat ttgcccatca ggatccggtc
    16861 gccttttttc acttcgagag cgacgcgttt gccctgatca tcaatacggc cgggacctac
    16921 cgcaatcacg atcccttcct ggggtttttc cttggctgta tccgggatga taataccgcc
    16981 gcgaactgtt tcgctgggct cttcacgctt gacaaggata cggtctgcaa ggggacgcac
    17041 tgtcatggtg tgactccttc ttttaacgtt tgtttttccg gttgcttcta tccaaccgac
    17101 actgaaaaaa cccgcacgct ccactgcgcc gggcgccaac aatcccgggg cggctgttag
    17161 cactcacccc gtgtgagtga taataacaca gacaattcat ccttgtcaaa cccgaaaaca
    17221 gcagacagaa atggacccgg ataaagggcg gcaatttctc ccttcctaca gcctctgatc
    17281 agaaattact ttgataagca gggagaccgg gtagattttc cttcgggttc ttaaccatac
    17341 tatggtctgg cgtacaatgc atgtatcttg cattttgcgg tccgctaaaa aaggaaaggc
    17401 ttttgtgatt atccgcgatg cccgaagcta tacctattcg ctgcagctga aagctccttt
    17461 gcgacggaaa gaggaggtgg tggtgcgacg gaaaggcgtt ctttttttcc tgcagggcga
    17521 agaggatttt atcggctggg gcgaagcggc gccccttccc ggcttctccc gtatcactct
    17581 tcaggaagca caggcactgg gcagagccgc agcaaaggca gtcaccggcc ggagtattgc
    17641 agatgcccgg aactggctca ccccggaaca atcgggcgat ccctctgttt actttgcttt
    17701 tcatcaggct tttcgcgtgc tggcacagga gcacgaggaa gaaaaaaagg attcctgcac
    17761 tgttgcgctg tgttcccttc tggatggtga cctcaatgaa actgaagccc ggctgcaggc
    17821 cggcttggca gaaggctgcc gctgctttaa attaaaagtg gcgcgacgct ctcttgccga
    17881 agaaacagat ctggtggcgc acatcctgtc tcagcttcct tcagacagta cgcttcgtct
    17941 tgatgcaaac cggggctgga cttatgacga ggcgcttgct ttctgttgct ctgtgccttt
    18001 ggaaaaaatc gcatttattg aggagccgct tcaggactgg cggcaaattc cccgcctgca
    18061 gacagaaagc gggattgcct gtgctgttga tgaatgcctg caggacatga gcgcattgct
    18121 tctctttcgt gccgaccatg accgggaagc gctcatgcat gcataccgga cagtggtgga
    18181 agcggcttgt tatttggtct ggaagccctc actctccttt cctcccgaag ccctggggct
    18241 tgatataaga aagcgatgta tcctgagcgg cgcttatgag agtggcgtgg gtacagcggc
    18301 cgtactggaa gaagcactgc gtcgtggcgg agcatccctg gcggcaggtg tggataccta
    18361 cagccgcctg gcggaagacc ttcttctgac cccgcttcat ctggaaggga accgggcaga
    18421 cgcccggctg tttattcctg cggggcaggc ggtggatgtg acgcatctgg aggaatgctg
    18481 ctgtgaataa ggcatccgct ctgtcttgtc cgatccggga acaggctctt aagcaccccc
    18541 gtatgcctgc tctgtgcgat gcgggcggtg tgcaggatta tgcaagcctg gatgcagcga
    18601 tagaacagta ccgggcgatg ctgggcgcgc agcattgcaa agcaggagac cgcatactgc
    18661 tcaccggcac gccttcctcc gcttactgcg ctctgctctg ggcatgcttc agggaaggca
    18721 tcaccgcctg ccctgtaaat tctgcgttac cctctgcagc gatggagcat tgggcgaggt
    18781 tcaccggtgc ggcggcagtg tacggtgagc acgccgatct gcttcctcct tcactgcccc
    18841 gcctcctgtt ttcagggact tttcaggaaa cagaggagga gaacccggga aaaaaactgc
    18901 ccctgaacgg actacagcat gctgtcatca ttcaaacctc cggcagcacc ggcacagcgc
    18961 gcggtgctgt gcttactctg tccgctgttc ttgccgctgc cgggtttgcc aaccaaaaca
    19021 tgccgctccg gcagggcgac cgctggctgg tcacacttcc tttttttcat gtctcaggac
    19081 tgtccattct tttccgctgt gcggcagcag gcgcctgtgc cgtgtttcct gaggcaggca
    19141 gtacattggc gcaaggactg cttgcgcatg atgccacaca cctttccctg gtacccacac
    19201 agctgcaacg ccttctggat gatccggaag cagtcccttg ccttcgaaag atgaaaggtg
    19261 tgcttctcgg cggtgccccg atgcctgaat ctctgctgcg ccgtgctttt gaagcaggaa
    19321 ttccgctgat ccccagctac ggtatgacgg aaacagctgc gcagtgttgt gcggttccgc
    19381 caggttccga tttcagcctg ctgcaaacga gcgggcgtcc cctgcagccg ggctccatgc
    19441 gtattgatga gcggggcatg atccagtaca acggggatgc tcttttccag gggtatctgc
    19501 agggagatgg aagcctgcat ctcccgctca gcactgacgg ctggttcccc accggggact
    19561 gcggcttctt tgatgaagcg ggttttttgc atgtcaacgg cagggcagac agcatgatca
    19621 tctgtggtgg cgagaacatt caccccgaag agattgaaca actcctttgt actgcgccgg
    19681 gcattcgccg cgtcctggtt tcaggggtaa cccatgctga gtacggaacc attccggtcg
    19741 cctttatcga tcctgaagac ggagccccca ttgacgaggt ttttctgcgc cgttatctcg
    19801 ctgaaagact gccgcggttc aagcatcccc gccgttttct tcgctggccg gatgcggaac
    19861 cgggggggct gaaagcatcc agatcctggt ttgcttcgga agcggaaagg cttcttgagg
    19921 agaaataagc ggcatggtac ccgcaggaaa gcccggatct gcagcacatc aaaacactgg
    19981 acaaattccc tctgagaggg tatagtattg aatacatgtg ttttttacct ctgctcgggt
    20041 ctgcatcatc atttataaag gatttgccat ggtggagaaa caaatactca gcggttgcgc
    20101 ctgctgcggg atcagcaggc gtcatttttt ggcagccggt tgtgccgcct gtgccggagc
    20161 ggcggggctg gcgcttccag tacgccgggt ttatggagca ggcaatagca gcaaaggaac
    20221 cctgcgtgta ctttttgcgc ttcacgcgga aacacagccg ggccccgact ggcccaatgt
    20281 cggcttcgag ttccgcccgg tcatggaccg gctcatgaca caactggaaa aagaatgcgg
    20341 tgaattccag tatctgccag ccatggtaaa cggtgcggaa gcgacccagg cggttatcga
    20401 tgaagacaaa gggaaagaca tcctcggcta tctggtcttt caactgaact gctggaatcc
    20461 ggtagcgcag acagcagcca ctcagggtaa gcccgtgctg tatgtcgatt tcaaatacgg
    20521 aggcagcggc ggctttctgg tctacacggc gcactttctc cggacgcaaa gcggcaatgt
    20581 cggctttgtc gcctcttcgg acccggcaga tctaaccgct gcggtacgct gtttcaatgc
    20641 cgtggctgaa ggtgctcctg cttctggttt tgcgggtctg gttgcccggt gccgccagga
    20701 acgcacagcc gcacggtcag acacagcatg cctgaacgat cctgtgaaga ctctgcccgt
    20761 tgcggaatgg aaggaacgta ttgcgggctc aaagattctg ctttatcagg atcaggaaag
    20821 tgccgatctc gggacgtaca tgggcatccc gcttatgaaa cgtccttttg cagagctgaa
    20881 cgctgcctgg gaaaaggccg atcgggagga atccgcgcat cttgctgaga aatgggtacg
    20941 cgaggcgaaa gcggtggaag gtgttccgat ggaggtgctg aagcagtcgg ctgccatgta
    21001 tcttgccaac aaagcgctgt taaaagagca tggcgccaat gccatcacca tcaactgtct
    21061 gggtggattt tacggcgggc atatccatgc ctatccctgc ctgggcttcc atgagttgtg
    21121 caatgaaggg ctggtaggcg cgtgtgaagg ggacgtgcgc tcaacagcga ccatgaccgc
    21181 cttcggcatt ctgagcggcg gcagaccggg tatgatttcg gatcctgtga ttgacacatc
    21241 gaaaaggcag attatttacg cacactgcgt cgcctcaaaa cgtgttttcg gtccacaggg
    21301 cagcgacaat ccctttacaa tcctcaccca ttccgaagac cgtcagggcg ccgctgtccg
    21361 gtcgctgctg ccgtcggggt atctgaccac aacgctggaa gtgaacccgg acgaaaaaga
    21421 aatccttatg catcaggcaa aggcggtgga caatgatcct gatgaccgcg cctgcagaac
    21481 caagctgtgc gcggagccac tgggtgacat ggaaaaactc tttacccaat gggatcgcta
    21541 cggttggcac cgggtcactt tttacggtga tcttcgtgag gctgttcatg aacttgccga
    21601 tgccatcggg tatcaggtgc tggaagaggc ataaccgggc acaaaagcca ggtgacaaaa
    21661 actagcggct atccgcttca ccggcggaaa gaatagcgat tgcctgcttc aggacaggat
    21721 cctcttcctt cctgaagagc gattcacatt tttccatggt ataaggcacc gtcacgtccg
    21781 gtgcgaccgg actgacacgg ttcgtgccgg ctgtcacaag aaccgagccg tcctcttcga
    21841 gcatgcgccc ccggggatag tgaatcacat gcccgccggg catggtgatt gttccatcca
    21901 taagtccata gctgccttct gttgctgtag agcccaccag ctgaaccttc ggcatttttc
    21961 ggagagcggc tgccagcgct tcgccgcctt cccgggtatt gcgattgatc agaacacaga
    22021 caggtgcgga aaagcaggga gagcgaggct ccacatagag gttcagtgac tcattgagag
    22081 caaagccccc tttcccggag tcaaaagaaa ccactttccg gaaggacagg ggcgtttggg
    22141 taaagtgccc cgcataggca gctgccagtt cgtcaagccc gcccggattg ccgcgcaaat
    22201 ccataatcag gcccgtaaca cgggcctgca caaatttttc catacttttg cgaaaggcgc
    22261 gtacaggaaa aggcattgcc agcgtggcgg catgacagta gatgcggata taaccgatgt
    22321 ttccgtcgag aatcctgctt tccagaggag actcaaactc cgtaaaactt ttcccctgat
    22381 cggaaaggtt cttgagaccc tgcataaaat cgcggcgtgc ctcaagccgg gtgatccaca
    22441 gatcaccggc accgggattg cggaacatga actccgcagg ggtgccgaca gatgcccggg
    22501 tcagcagggc acactgaaca aacttctcgt tctccaatgt ggcgacaggc acatcattcc
    22561 ataaaacagg ctgctgtttc agggcttcat ttatgggcag tccgttccat tcaagaattt
    22621 cggcgcccca ttgaatcccc gttactgccg cctgactctc gggatccagc caggctatga
    22681 ccaccctgcc gtcttccaga ggaagaagag aaaatccgta accgccgcct atatggcttt
    22741 ctttatattc ctgcggtgta gtgatgctca gcgagccgtc gggaatggaa taaatatatt
    22801 cgcgcagggt taaataatag gcttccgcat cgttattcgc tgatgcttca tccagccggg
    22861 ttttaaagcg gtcataaagc gcatcccagt caacgccctt ccactcggtg aaggcatatt
    22921 cacgggacag tttctggtgc attgcgtcaa aggcggcttc ccaggatcgc ccgctgaaat
    22981 taccgtcgct gtacccgccg aaaagcgctt tgacagtgct cagtgtcaca ggcgactgcg
    23041 gcatgggcgc gcttattttc gtgagaggca gcgtggaatg aacacagccg gacagaaaaa
    23101 gcgccagcag cattgcagga ataaaactat acacttgttt tttggtcatg atacgccgca
    23161 tgtgttggat ccggccaaaa gagccgacac gagataatgg tctgtttaca cccctattgg
    23221 tggtattgta ccatagttat ccggtctttc gtgaaaaaca aacagctctc ctgccggacg
    23281 gtacgctgac aaccctgaaa agaaacagat tatggcaatc aaaccttcgc tcatgaccac
    23341 gaccctttgg gattttccat cacagcagta cggcaaagaa acccagggaa gcaaacagta
    23401 caaaggtgca actccggcgt atatccagtg gaacctgatt cagcggtaca cccgtccggg
    23461 agagctggtg gtagatccca tggcaggcag cggtaccacg ctggatgtgg cacgggagct
    23521 ggacagacgt gcactgggct atgatgtcaa tgtaacccgg aaagatattt tcaactgtga
    23581 tgcacgaaag ctgccgctgg aagatgaaaa ggcggctttt gtatttgtgg atccgcctta
    23641 ctcaactcat ataaattaca gtgacgatcc gcggtgtatc ggaaaactgg atgcacggga
    23701 aaacgcttat tatgaagcga tggatcaggt aatcggtgaa atcgcacgca ttctccggcc
    23761 tgggcggcat atggcgctgt acgtcagcga ctctctcgta aaaggaaaac acttctgtcc
    23821 tatcggattt gaactcttcg gactgctgcg caagcgtttc acccccgtgg acattgtttg
    23881 cgtgacccgg cacaacaccg atctgtcaaa aggaaattac cacaaggcag cccaggaagg
    23941 aaactttctc ctgcgcggtt tcaactatct ttttatcatg cagaagcctc tgcgcccggg
    24001 ttgacaactg ttttttttgg gtatctggat aaaaatttgc actttgccgg gattatcttt
    24061 ataattccga ttgttttact tgtatatgaa cgctgcgtcg gcaaaccacc acttcaactg
    24121 aggagaaaca tcatgtccac cgaatccaat tcataccgtc ccggaacact tgcccttcat
    24181 gcgggccagg aaccggatcc caccacgggc gcccgtgccg taccgattta tcagaccagt
    24241 tcctatgtat ttcgggatca cgatcatgcg gcgaagctgt tcgcactgca ggagttcggc
    24301 aacatctata cccggctcat gaatccgacg acagatgttt tcgaaaaacg tgtggcggcg
    24361 cttgaaggtg gtgttggcgc gctggcgctt gcgagcggtg ctgcagccat cacaacggca
    24421 gtgctgaaca ttacccgcgc cggtcagaat tttgtttcct cccagacact gtatggcggc
    24481 acctacaatc tttttgcgca tactttaaag gatctgggta ttgaagcgcg ctttgtggac
    24541 gcttccgatg cttcaaattt cgagaaaaag attgacggca acacccgttt tctctatctg
    24601 gaaaccatcg gcaacccggc gaacgacgta gtggatattg aagccgtgtc ggcagttgcc
    24661 cacaaacatg gcattccgct gattgtggac aatacggtcg cctctcccct gcttttccgt
    24721 cccttcgacc acggtgcgga tattgtggta cattcggcga ccaaagtact cggcggtcac
    24781 ggcacctcca tcggcggcgt tctggttgac agcggtaagt tcgactggga caacgggaag
    24841 tttccggagc tgacggagcc taacgacagt tatcacggca tgaaatttta cgaagccttt
    24901 aaaggcatcg gcaatatcag ctatatcatt aaagcacggg tcaccctgct gcgtgatatg
    24961 ggcgcctgcc tgtcgccttt caatgctttc ctgctccttc agggcatgga aacgctgcac
    25021 ttgcgtgtgc cccggcacag tgaaaatgcg ctggcaacgg cacagtttct cgaaaaacac
    25081 ccggcagtcg cctgggtcaa ttaccccggt cttccaagcc atgccgatta cgcccgtgca
    25141 cagaagtatc tgcccaaagg acagggcgcc attctgggct tcgggattaa aggcggttcc
    25201 ggggcggcag tgaaattcat taattcatgc aaacttgcat cgcatcttgc caacatcctc
    25261 gatgccaaga cgctggtgat ccacccggca acaacaacgc atcagcagct gtctgaggaa
    25321 gagcagcgcg ccgccggcat ttcacctgaa tttgtgcgcg tctccgtagg tctggaagat
    25381 atcgcagata ttctggagga tttcgatcgg gcactgaccg cctcgcagca gtaggtctgt
    25441 acgccgtctg caggcggtgc cgatcttttg aaaaagcggt gataagattg ttccggggaa
    25501 gtactttgtg cttaaatgaa agagaagcgc aaaggcttcc ccttttccat tcttttaaaa
    25561 agggttgccc gcccatcctg attttcagtt cattcccgct tcgccggata gagtattgtc
    25621 catgagtacg gaaacaacca cagagaccgg gtctgtcggc ctggtgcaga cccaaagcct
    25681 tcttctcgag gaaacccggg acggcttcac cctggaaagc ggtgcggcca tcggaccggt
    25741 gaccgtcgcc tatgaatgct acggcacgct gaacgaggaa aaaaataacg ccattcttgt
    25801 ctgtcatgcc ctctcaggtg atgcacacgc agccggacgg catcgggaaa acgatcccaa
    25861 gcccgggtgg tgggacggta tgatcgggcc gggtaaaggc attgacacaa acaagtattt
    25921 tgtgatctgc tcgaactttc tgggcggatg caagggaaca accgggccga tgagcctgaa
    25981 tccggcaacg ggaaaggcgt acaatcttga ctttcctatt ttcaccgtcg gcgacatggt
    26041 acgagtacag catgcactga tccgccagct cggcatcgat cagctgcttt gtgtcctggg
    26101 cggatcgctg ggcggtatgc aggcactgga atgggcgacc cgctatccgg atgatacccg
    26161 ttccgttatc ctgctggcga caagtcacta tacaggcgcc cagcagattg ctttcgatgc
    26221 cgtgggcaga agcgccattc aggcagaccc tgaatttcac ggcggcgatt ataatcccgc
    26281 gcggggcccc cgaaaagggc ttgccattgc gcgtatgctc gcccatatca cctatctgtc
    26341 cgatcagagc atggatcgca aattcggacg tactttgcac cgttccggat cgctgggcta
    26401 tcgtttcggc agtgaattca atgtggagac ctatctggat tatcagggcg aacgctttgt
    26461 caaccgtttc gacgccaaca cctatcttta tgtgaccaaa gccatggatt atttcgatat
    26521 cagcaacggc tttgagtcgc tggacgcatc gctggcgcgg gccaaaccgc ggacactggc
    26581 aatatccttc acctcggact ggctgttccc gccgcacatg tcgcaggata ttgtctatgc
    26641 gctcgcccga aatcagaaag aagtggcgta ctgcaatatc cgcactgatg cgggccatga
    26701 cgcttttttg ctggagctga aagaaatatc ccggctcatt tcaggtttcc tcatccatac
    26761 cctttacccc gaaagagact gcgagaactg tcctccgaag ccatgcgacg gtgcgcaaga
    26821 ccaggtgccc attaagaacg accggctcgg caaactcaag cgggtcgatt atgaaatgat
    26881 tgtggatctt gtggaagaga acagtcgcgt tctggatgcg ggctgcgggg aaggcgaact
    26941 gctctgcagt ctgcagcgac ggaagaacat caccggagtc ggtatagaac tcgatcagga
    27001 aaaagtgatt cgtgccatcg gctgcggtat ccatgtgatt caggcgaatc ttgacaaagg
    27061 gctgacccag ctgcccgaca actcctttga ttatgtggtg ctgagcatga cgctgcaggt
    27121 ggtgaaaaac cctgccttcg ccctgaaaga gatgctgcgg gtcgggaaaa aatgcattgt
    27181 caccttcccg aatttcggtc actggcgggt gcgcaccaat ctggcattca aaggaaaagc
    27241 gccggtaaca ccgaatctcc cctactcgtg gcatgactcg ccaaatcgcc atgtcctgac
    27301 catcaaggac ttccgccagt tctgcgacag cctcggcatc ggtatcgaac aggaaatagc
    27361 gctgaatgcc gacggacgtg cccggcttat gccgaatctc tttgcggaag aagcggttta
    27421 tgtgctgacc aaaaacggct tctgacaaaa aagaagaagt cttccatcgc tcggacaacg
    27481 gcgccgcatt ttgcaatgga tccatcataa ttggcatagt accccttctt tatgtcgtgt
    27541 gcttacatgc ttactcagga ttattttccg catgccgttc aaaactcctg tcccggttac
    27601 gatgcggtcc attgggtgga tcccgctgct catctgtttt tttttctgca gtggcgcctc
    27661 ttttgctgat accgccctgc gtcaggccgc atggcttcgt gatcctgttt ttcagggcgt
    27721 gccggtgctg aatctttttc atcgggaaaa agatccggcg cctaaggctg aaggcccggc
    27781 gcacgtacat acacttttcc ggcgggaagt aacgcttgcg gatacgcccg tacgtgctat
    27841 tctgctgatt acggcagatg actacgttaa gttttatatt aacggttatt ttgcggtaca
    27901 gggtccggca ccttcctatc attttgcaca tcattgcctc gaaatggacg tcagctcttt
    27961 tctgaaaaaa ggcaggaaca gtctggcggc gcatgtctac taccagggac ttgtgaatcg
    28021 ggtctggaac agcggcgaca accggtcggg ctttgcagcg gtgctccagc tcacctatgc
    28081 ggacggacgt accgatgaaa ttgttacaga tgaacagtgg cgctgccatt cgcttcaggc
    28141 ttttaccacg ggggaaatca tcggctaccg tactcagttt caggaaaata tcgacatgcg
    28201 cctgatgccg cgggggtggc gtacaacagg atttggtgat ttatcgtgga caacaccgct
    28261 ggcaggcgga caggaccatg tgttcatcgc ccagccgaca ccgccccttc aggtcagtct
    28321 ccgggagccg gcccgaactc aggaaggaaa aaaaggacgg tatctctacg atttcggtca
    28381 aaccatcgtc ggagctgctc agatccgttt gcagggaaaa aagggacaca aaataatcgt
    28441 ccgccatggg gaagaactgc agccggacgg atgggttcgc tatgaaatgc gcgccaactg
    28501 ccgctatgag gaaacatgca tcctctccgg cgatccggat ctgattgagt tttatgatta
    28561 ccgctccttc cgctatctcg agattctgaa tgcgccggag gcaccggaag tccgggtggc
    28621 tgtgcgtcat tatccttttc agccggaggc tgtgtctttc cgctctgaaa acaaactgct
    28681 ggaacagatc tgggcaatct gccagcaggg tgttatcatg ggatctcagg aagtatttgt
    28741 cgactgcccg tcccgtgaaa aaggacagta cctgggcgac acgctcatca cagcccggag
    28801 ccatatgtgg cttacggcag accctcgcct gaccaatcag gcaatccgcg cttatgtcca
    28861 ttccgcctgt gtgcatcccg gactgatggc agtggcaccg ggcagcttta tgcaggaaat
    28921 tgccgagtac tcgcttcagt ttcctctgct gctgctccac tactatcaga tgagcggaga
    28981 ccgtgacact gttgaaaaag cagttgattc cgtattctcc gggctcttcg actactttgc
    29041 aggctttgag aatgagcggg gactgctggc aggcatcagc aaggaaaccg gcaaatgggt
    29101 actggttgac tggccggaaa atcttcggga caactacgat tacgagtatt cccttcaggc
    29161 aggaaacacg gttctgaacg ctttttacta cggtgcactg cggacagcgg cggcactgga
    29221 gcgcatcctc gggcggaacg gcaaaaccta tgatcagcgc gctgaaaaac tggcggaaag
    29281 ttttgcgcgc catctggtca accccgccac cggactctat gtcgatgcgc cgggatcaca
    29341 gcacagttcg ctgcatgcca atgctgtgcc gctttttttc ggattgacag caggcgccga
    29401 caaagatgcc atgctgaacc tcattagaga aaagaaactg tcctgcggcg tatatatggc
    29461 atcttttgtg attgaggcgt gcttcaaagc gggcgcaccg gaactgggct tttccctgct
    29521 ctgctctcag gatgagcatt cctggcatga aatgatccgc cacggtgcca cgacctgcct
    29581 ggaagcctgg gcgcccgatc agaaatggaa taccagctgg ctgcatccct ggagcagcag
    29641 ccccatttat ctgcttgctc agtatgtttt tggcataagt ccggcagaag ccggatggaa
    29701 gcgcatccgg tttgctccgg cggaaatccc cgggctgccc gccatgagtc tgcgtctgcc
    29761 cttacccggc gggcggtctg tgtcggtaaa ctatgatccg gaaaaaggat acagcgccat
    29821 gtttccccca gatatttcgg tagataagaa cgcagctccg ggatcgaaaa aaggtatcgg
    29881 taccgaagcc ctgaaagcag caaattggca ggaacgggtg ggcgctgcac ccggcttatg
    29941 ggtcagcatt cccgaacagc gactttacat cattgaatcc ggacaggtgc tggacacgat
    30001 ggtctgctcg accgctcttc ggggcatggg ctctgtcatt aacagccagc agacaccacc
    30061 cggctggcat cggatcacgg gaaaaatcgg agacggcgaa ccagtcggca gggtcttccg
    30121 ttcacggcag gcaacaaaag aactgtggaa gcccggcatg acctccaaag aggatctggt
    30181 ggtcacacgg atctttatcc tcgatggtct ggaagacggg ctgaaccggg gaaaagacga
    30241 tcagggaaga atcgttgatt caaggcagcg gttcatttat atccatggaa ccaatcatga
    30301 ggaggagttg gggacgcccg gctcacaggg ctgcatccgc ctcgccaatg atgatgtagt
    30361 gactctattc aaccggattc cggaaggcac gctggtttat attgaaccct gaaactggaa
    30421 agaggggctg cctgatgacg ctcagctgcc cggcgcatct tcgacaatgg caacgccagc
    30481 actggtgccg atacgggtcg ctcccgcacg gagcatgcgc atgacatcac cataactgcg
    30541 gattccgcct gccgctttta cgccgaggcg gtcaccgaca gtctcacgca tcagccgcac
    30601 atcgtccacc gtagcacccc gggtaccgaa ccccgttgac gtcttcacat aagccgcacc
    30661 ggcacgaacg ctcatacggc agacttccac cttttcatcg tgggtaagat aactggtctc
    30721 cagaatgacc ttaacgggcg ccgttccggc ggctttcact acagcggcaa tttctcgttc
    30781 cacataagag ccatagccgg attttacggc gccgatgttg atcaccatat ctatttcggt
    30841 agcaccgtta ctgatggcat cccgtgtttc ctccactttc atatgcgccg tattggcacc
    30901 aagagggaac cctatacagg catcaatacc gactgaactg ccctgcagct gttttttgca
    30961 aaaggcgacc cagacaggat ttactgaaac ggtccggaaa gaaaagcgga cagcctcttc
    31021 gcacagggct ttcatctctt cttcagaggc aaaagccttg agcagggtat gatcaatgac
    31081 tttggctact tgaatacggt tcactgaaac agcaagcctc cgactcggca gtattttgtg
    31141 tggttagatg gacaaaacag gtgttgcggg cattgtaaca cggaaagcaa ttccggggca
    31201 tctttccggc tacggcactt tgtgtcgtga attcacccgg gcgcagtttg cctctctttg
    31261 cagtattcag gtacagtaat tttctgcagg cacacttttt tcagcataat cgatgtgctg
    31321 gatcagaatt cattcaacag gatatttata tgaaaaaaat acgcgtgggc gttgtcggaa
    31381 tcgggcatct gggctatcat cacgcaaggc tctaccggac attggagcag gcggaactgg
    31441 ttggcgtggc ggactgcagc gaagcggcac ggaacagggc ggcggaagac ttcaatgtaa
    31501 agactgtttc atctgtggaa gccctgctgg atctgggggt ggatgctgtc tccgttgcca
    31561 cacctactga aacgcattgt gaaaccgtgc ttgccgctct cgcacagggc gcccatgtac
    31621 tggtggaaaa gccccttgcc tccacgattg aagaaggaga gcagatggtc gaagcggcag
    31681 cggctcaggg gcttctgctg caggtcggtc atgtggaacg cttcaacggt gcggttcagg
    31741 cgctcttcga tgcaggcatg acaccgaaat ttatcgaatg ccatcggctc agtccttttc
    31801 cgggacgtgg cagtgatgtg agtgtggtgc tggatctcat gatccatgat ctggacattg
    31861 tcatgaaact cgcggattct cccgtgatca gtattgatgc cgtcggtgtc ccggtctttt
    31921 cggcgcagga ggacatcgcc aatgtccggc tccgctttca gtcgggctgt gtcgccaatc
    31981 ttacaagcag ccgtgtttcg ctggagcgga tgcgtaaaat ccggattttc gaccctaacg
    32041 cttatgtatc cacagactac agtgctcagg aggttctggt ttatcggaaa aaacccggta
    32101 cccttccacc tgatgccaat cccatggaat cgattgagat cacgccgctt gcggtaaaaa
    32161 aggaagaacc gctccgtatg gagcttgcct cttttctgga acatctgcag agtaacactc
    32221 ccgtagccgt cagcggtgaa gacggcttgc aggcactgaa gctcagctgg cagattctgg
    32281 aatcgataag gaagaacggg tgaaaacaat ttttctcatt gcaggcgagt cttccggtga
    32341 tattcacggt gccaatctga tccgttccat caaaaagaaa aatccggagg tgcattgctt
    32401 cgggctgggc ggccaggcga tggcggctgc aggtatggaa ctgattcacg accttgcctc
    32461 cgatgcaatc atgggctttg tggaagtcct gcgccgtgtg cgaccgctgc gccgcctctt
    32521 tctggatacc gtagccctgg tcaaagaacg acgccctgat gtgatcgtgc tgatcgatta
    32581 tcccggattc aatatccgct ttgccgaagc cgtgaaagag ctgggaattc ccgttgttta
    32641 ttacatctgc ccgcaggtct gggcatggaa gaaaaaacgg ctggacacaa ttgcccgtct
    32701 cgtgcgcaag gcactggtca tcttcccctt tgaaaccgat ctctatcggg acaaaggcgt
    32761 atactgccgc tatgtcgggc atcccctgct ggatgaactg aaagacagcc ttcattcctt
    32821 tcccgaacgg aaagactgtg tgatcggcat gctgccggga agccgctcac aggaaatccg
    32881 gcgcattgct cctgttatgg cgcaaatatg cggcgatata ctggcggtgc ggccggaagc
    32941 acgatttctt gccccttgca ccaatgaagc gcgggcggcg gaagtgcgtg ccgccttctc
    33001 cggccttcct gttgaaattg tcgtgcggcc tgcccgggaa gtctttctgc agagtcgctg
    33061 ctgtctcgtt gcatcaggca ctgccacgct ggaagcggca ctttttgaaa caccgctggt
    33121 gatcctttac cgggtgaacc ggctcagtta ctggcttgca cggcttctgg tccgcattcg
    33181 ctttatcggc attgtcaata ttctggtagg ctccgaaatt gttcccgagt ttattcagta
    33241 cgaggcaacg tcacagaaga ttacccctgt gctgcttgaa ctgctggaag acagcccgct
    33301 tcgcagtcag atgcttgcta atttctgcat gctcaaaaga catttgggtg gcgacggagc
    33361 ttccgacagg gcagcagatg aaattctgac actgctgtaa cctccacgca tagaatcagg
    33421 aaaggtatat tggaatgaaa atgctgccgt attttctcgc tgcgctcctg ctgttttcag
    33481 cgacttccgc tttttccgct gcggagcgaa ggcacgacct cgtgatttac ggaggcacct
    33541 ccgccggagt gatagccgcc gttcaggcgg tacagatggg aaaaagcgca gttatcgttg
    33601 cgccggaacg gcatctgggc ggtctgacag caagcgggct cggctacacc gacactggca
    33661 acaaagcagt gattggcggg ctgtcccgca acttttatca ccgggtctgg cagcactaca
    33721 gccagaaaga aaactggcgc tggcaggaca gagaaagttt tcgtaaccgt gggcagggcg
    33781 ttcctgccat ggacggagaa ttccggacca tgtgggtttt tgagccccat gtggcggaag
    33841 atgtttttga agagctgatc cgtgaattta atattcctgt ctttcgtgat gaatggctcg
    33901 accgggacac tggcgtgatt atggaaggca accgtattac ttccatccgc accctttccg
    33961 gcaatatcta ccatggcagc atgtttatcg atgccgggta tgaaggcgac ctcatggcgg
    34021 cggcgggcgt ttcgtatacc atcggcaggg agtcgaacag catctacggc gaaactttga
    34081 acggcgtcca gaaaggaaat gccaaatacc atcagttcaa tgagtgcgtg gacccctacg
    34141 taattcctgg cgaccccgaa agcggactgc tgcccaggat ccataatgat gcccccggcg
    34201 aagacggcga aggggacaaa agaattcagg cctacaatta tcgtgtctgc ctgaccactg
    34261 tcccggaaaa ccggattccc tttccgaaac cggacaacta tgatcccctt cagtatgaac
    34321 tgctggcgag ggagctggat ctcggttttg aacggatgct ccggaaattc gatgctatcc
    34381 ctaatctgaa aacagatacg aacaactacg gttctttttc cacggataat atcggaatga
    34441 attatgaata tcccgaggct tcgtatgaaa agcggtttga gattctccgt gagcacagaa
    34501 aatatcagga aggatatttc tggtttctgt cgaaggaccc ccgtgttccc gagggcatgc
    34561 gggaaatcat gagcaaatgg gggctcgcca aagatgaatt cacagacaac aaccactggc
    34621 cccatcagat ttatgtgcgg gaggcaaggc ggatggtagg ggattttgtg gtttcagaat
    34681 tgcatctgcg gcgcaccatc ccgacacagc gatctgtagg aatgggttcg tataacatgg
    34741 attcccataa cacccagcgt tatgtggatc gggacgggca cggacgtgcc tgcgccagga
    34801 acgaaggaga tgtgcagatc aatccgggcg gcccttaccc catcgattat ggcgccatta
    34861 ttcccaaaga aaaggaatgc gccaatctgc tggtgccggt atgcgtatcc tcttcgcata
    34921 tcgctttcgg ctccatccgc atggaaccgg tcttcatgat tctgggtcag tcagcatcga
    34981 ctgctgctgt gctggcactg gatcagggaa tcagtgtgca gcagctggcg tacccggatc
    35041 tggaagcgca gcttctggca gacgggcagg tacttcatta cgagacacaa aactgaaaga
    35101 cttcagaaag agtccagtta agtgagattc ttctgaacaa agaagcataa tctctcaaaa
    35161 gaacttacaa gagaaggaga cttcagcatg agaaaccata gtatgtggaa agggaccgga
    35221 gggatagtgc tctccgccct gctgctggca ctttgcattt ccggcacggc ttccgctaat
    35281 aacctgcggg tcagcgaaaa cctgacaccg ctggatgaat atgtggctgc gcccgatgat
    35341 aattaccgct atgagctgcg ctacagcaag caggaaaaag ggttttcaga acatgtgctg
    35401 tatatggcct cgcagcggtg gcttacggaa gaactggtgg acagaacgct gtgggaacac
    35461 tggcttgtcc ttgtcgtgcc cgatgaaccg cagtgctcca ctgccctcct caccatcggc
    35521 agcgggaaaa acggaggcga catgcctcag ggtgcggatg ataccctgcg ccgcagtgcc
    35581 aaagccacca atacaattac cgcaaaactt tttcaggtgc ctaaccagcc cctttatttt
    35641 accgatgatg gcgaacggag gacggaagat gccattatct cccacaactg ggataaatac
    35701 ctgcgctccg gcgatcccat ctggcttacc cggctcccca tgaccaaaag tgttgtccgt
    35761 gccatggata ccattcagga tttctgtggc ggaaaagcgg gcggtgaaca taaagtggat
    35821 cagtttgttg tcaccggggc atcgaagcgc ggctggacaa cctggacgac ggcggcggtg
    35881 gacaagcgtg ttgtcgcctg cattcctgtg gtgattgatc tgctgaatgt ggtgccttcg
    35941 ttcatccacc attacgaggt atacggattc tgggcaccgg cagtcgatga ttatgtgaac
    36001 tcccgcattt tcgactggat cggctcgccg gaattcgacg ctctgctgaa aaacatcgat
    36061 ccgtacagtt tccgcgagcg tttgactatt cccaaactgg tgatgaacgg tgctgccgac
    36121 cagtttttcc tcaacgactc ctggcagttt tactgggatg atctgaaagg gccaaaatat
    36181 ctgcgttatg cgcccaatgc gggccatggc atggacaacg ccgatgcggc aggaaccatc
    36241 atcgcttttt atcagcatat catcgaaggg aagccgctgc cggaatacgc ctggtccttc
    36301 cctgatgacc agtccatccg ggtgactgcc aatacggtgc cgaaaacggt gaagctgtgg
    36361 caggcgacca atccggaaaa atgggacttc atgctgcagg aaggtgcgcc cgtctggaca
    36421 gaaacggtac tggaagcgca tgccgatggc gcgtgggtcg gccgtgtgaa ctcaccggaa
    36481 aaaggcttca ccgcctttct ggtggaactc acttttcccg gactccatga tgatgtgctc
    36541 gtactgacat cacccacacg ggttacaccc aacacaccgc agcatcatct ggaaatgccg
    36601 actgattttt cagcaggatt tcttcatcgc aatcctgctc cctgacagac aaacctgcaa
    36661 tccgtacttt ttcgtcgccg atgcactttg aaaacaagtg aatcggcggc ttttcttttc
    36721 tgtcggttgc atccgacagc ctgcccggcg tatcctgaac agggatcatc cggaacggac
    36781 atgcgaataa gagcgctgtc cggagataaa tgggagacaa ctgctatgct tgacagtttt
    36841 ttcggtatca cccggtcagg ctcaacactt cggcgggagg tggtaggcgg cttcaccact
    36901 tttgcaacca tgagctatat tgtttttgtt cagccgaccg tgcttgccat ggcaggcatg
    36961 aatctgggtt ccgtacttct ggcgacctgt ctttcttcag cagccgcctg ttttctcatg
    37021 ggtattctcg ccaactatcc tattgctctg gcgccgggca tgggtgaaaa tttccttttt
    37081 gttttcacca tctgcggaac catgggtttc gcctggccct ccgccctcgc cattgttttc
    37141 atttcaggcg cgctgttcac gcttctttct ctgttcagtt tccgtgaaaa aattctcgac
    37201 gtatttccgc ccagcctcaa acatgccatc ggaccggcta tcgggctttt tatcgccttc
    37261 accgggatgc agtgggcggg aattatcgtc atcagcccgg caaccatggt caccctcggt
    37321 ccgctgaagc atgcggcacc gctgacggca cttgccggaa tcgccgtgac aacgatcctc
    37381 cttgcccggg gtctgaatat tgccattctg gcaggcatcg catcaacgac cttgctgggc
    37441 gtacttaccg gcgtgatccc ggcaacactt cccgacattg ttttttccac ggaaagtttt
    37501 ttcaatctga attttcgtga cctgctggat cactggcaga atgcactgat tgccattgcc
    37561 ctgttcttct ttctggatct ctttgatacc gtaggcactc ttgtcggcgt aagcgttcag
    37621 gcgggatttc ttaccgaaga cggaaaactg ccccgcgccg gtcgcgcttt tctgtccgat
    37681 gcgctgggta cctgcatagg cgcacttttc ggcacctcca ccgtaaccag cttcattgaa
    37741 agcgccacag gcgtggcagc cggtgcccgg acagggctgg catccctcgt caccgggctc
    37801 tgcttcattg ccgccatcct ttttgcgcct ctggtcgcac tggcaggcac caatataggt
    37861 ccggactatt acgctcttct cgggctggat catgctgccg tcgccatgta ccccaccgtc
    37921 gccccggcac ttatcgttgt cggatttctg atgatggcac cgctgaaacg gatcaactgg
    37981 ggtgatgtga cggaaagtct gcccgctttt ttcactgtcg tcgtcatggt tttcggctac
    38041 ggtattaccg aaggtatttc cgccggatgc atctccttcg ccggcattaa agcggcaaca
    38101 ggaaagcacc gcgacgtgca tcccattatg tatgctatcg ccgcggcgct gcttttacgt
    38161 tatatctttc tcaaataaga tgtgtcaggg acggctcctc cgcgccaggc tctccggatc
    38221 ggctccagcc tgaagaggcc gcatccagaa gcggaaggtc tgcggtctgc agataatgag
    38281 gtattcctct tcgggccagg cgccccagct gtcattaccg ccgacgccct gctggcgcca
    38341 gtccacattc agcacaatct catcccgttc aggcaactca aagggatgca ttgccgcagc
    38401 cagatcttcc accgtatggt gcatggcatt gacaccgagc agatgcgctt tgtcggcaac
    38461 aatcagcagc ccggcgcctt ccgcattctg aagcgccgcc cagcggacat cttctttgtt
    38521 cccgctttct ccgggattgg cgtagtgttt atagaactga tcgcgaacag ttccgctgta
    38581 acgtcccacc agcgcatctt ttcgatcaat atacgtttca tgaggaccgc gtcccagcca
    38641 ggtgattctg tcaaaaccgg gattcagaac catctgcaca cccagcctcg gcagtttctg
    38701 ctgcttcgta ttgcgcggca caaaatcatt ttcgacccga atatcgccgt tattgaaaac
    38761 agtaaaccga ctgaaccagc ggacatcggc ttctttcatg gaaagctgct gctccacaac
    38821 cacacgtttc ggtgaagatt ctttcacgga aagagaatcc agcacggcac ctgcatgggc
    38881 tgtcttccag atgccctggc tcttgtcgat ggcacggccc cggtcgttat ccgtcggcgc
    38941 acgccagaaa tcaggtcgaa gcgggctatg cagcaattcc aggccgcctg aggtcatgga
    39001 caccagagca cctgtctttt tgtcaaaacg gagcgccagg tcgcccgtcg ccacaatgag
    39061 ttgttccgcc tcgtcgctga ctgtcacggc ctgaccttcc ggcgcggcaa gaacaggtgc
    39121 cggtgcagcg tcaggcagga ggaactgatc ccacgccact tcgtgccctt ttttcgccca
    39181 cagttcatcc tgcgccagac ggaaactgag tgtaagaaaa tactcaacgc cgccctgcgg
    39241 ttcaaaatct gccacaggga tggtcagcgt cgtggtattt tgcggctcca gtgaaggggc
    39301 tgcctgcgtt ccttcctgaa taacggctcc gtctgcgctc acctgccaat gcagttcgag
    39361 ccagtctttc agattgacaa agtcatacca gtttttaact tccacctgcc gtgccgcacc
    39421 gtcaaccagc ttgctgtgga tatactgata gacatgtttc acctgataca ggccgggatg
    39481 gggttcgcga tccggtgtga ttacgccgtt acaattgaag tttttgtcgc tgggcacacc
    39541 ttcgggcccg taatcgccgc cgtaagacca gaagaaatct tctccctctt tagctccttt
    39601 gtaggtacgt ggcggcttct gtgccgttga ttcacgcaat gcctgatccg cccagtccca
    39661 gatgaaaccg ccctgcagat agggcttgtt ataaatgaga ttccagtaat cccaggcgtt
    39721 tccggtgctg ttgcccatgg aatggagata ttcacacagg ataaagggcc gtgtccgcgg
    39781 ttctccttct ttcaggagga attcacgccc ccagccgcca tccacttttt tgccgtccgc
    39841 ataatcacgc agcgccgcag ggctggaata catggggcag taaatatctg tgttccggtg
    39901 gaaccctgcg cgttcatact gcacgggacg accggggtcg cgctctttca gccagtcata
    39961 ggttgccatg aaattgggcc cgtttccggc ttcgttcccc aaagaccaga taatgatgga
    40021 ggggtgattc ttatcccgct caagcatccg tacggtacga tccatatgag ctttcatcca
    40081 gacaggcgga ttggcaagag attctttgcc gtaacccatg ccatggcttt caatattggc
    40141 ttcatccacc acataaagcc cgtattcgtc gcaaaggtta taccaggcgg gaacgtccgg
    40201 atagtgcgaa gtgcgaactg cattaacatt aaactgcttc atcagcagaa tatctttaat
    40261 catgctttcc cgggtcatca cctgtccccg atccggatcg gattcgtgac ggttaacccc
    40321 tttaaccaaa atccgctttc cattgaccag caggttaccg tctttaatct ccacacgacg
    40381 gaagcccact ttggagggca atacctcaat aacggagccg tctgtgtttt ccagactcaa
    40441 aagcagtgta tagagattgg gggtttccgc cgtccatttc agtgggtcgg aaatccgggc
    40501 ggaaaggctt attttttggc cgtcccctct ggccgatgcc cggacgtttt ctgtaaaagt
    40561 gcacacttgc ttttggttgg catcaagaag cctggcgcga acggtcagag gaacactctc
    40621 tgccgtatga ttttccactt ccgccttcac ggtcagctca gcatctttat acgcatcatc
    40681 cagcagggtg ctcacttcaa aatcccgaat atggacctgc gccggagacc acagatagac
    40741 atcccggaaa ataccgctca tacgccagaa atcctgatct tcaagatagg acccgtcgca
    40801 ccagcggaaa ttttctacgg caataatgtt ttcgcccgct ttaagaaatg atgaaatatc
    40861 aaattcaaca ggggtacggc tgtccttgcc catgccaaca aactggccgt ttacccagag
    40921 gtagaacatt gaattcacgc catcaaaggt aatcaatgca cggcgcccca tccattcttc
    40981 gggcacggta aagcggtgac ggtaggagtt gaccgtattg ttcgggtcgt cttcggggac
    41041 aaatggcgga ttccacggac ggggccaggg aaacggaatg ttgacataaa tggggatgcc
    41101 gtagccgtgc atctccacat tggcaggaac cggaatggtg gtccaggcac tgtcatcaaa
    41161 atctttttcc cagaaattcg ccacccgctc cgtgtgattt ttcgcatact tgtatttcca
    41221 gtctccgttg agagagcggt aaaaaggcga cttcacccgc tccgcattgg atacttcgcc
    41281 gatctgaagt gccgtatccg catccgggca gatgaccatg gtcgcatgag gcgccagcgt
    41341 gttgatgccc agcagttccg gattctgcca gtccttcaag tcttcagctg cgcaggcgtg
    41401 catgcacagg cttccggcaa ggagcagcgt cagacaaaat agcggcagaa caaaccctgc
    41461 aagaatttga aagggtgcat gaagggcgaa agagggttgc atggagagta ctccttccgt
    41521 aaagatagtg acagatcaac aggaaaaata tgctctatga ttctacttgc tctatcccct
    41581 gtaaatccag cacaggaatc acgcattcta aaatcttacc aggaataaat atcgggtagt
    41641 tgggataagt gacggcgcgc gtaagaatgg cggaggagat gcgtcgcagg gcgtatttct
    41701 gcggcgaatc tgagtggctg ttttcgtcat gacccgcttg agtgccaatg cggagattgg
    41761 cgttcccagg ggaagaattg cctttccacg tcatccctgc atccccgtca ttcctgcctt
    41821 cccacgtcat ccctgccttt ccacgtcatt cctgccttcg caggaatgac gggagggagc
    41881 aagaatgacg ggaagtgcgg aaatattgcc tgagacgagg cgcgcggtgt gcaggctttt
    41941 aggcataact cagatccaga tccgggagaa atgaaggaac agaccccgaa gtaaattatt
    42001 ttttgctttt agccttagtc tgtctctgca tttttcaaaa aatctgtttt gactgccaca
    42061 atgtgctagt cttgtgctat aaccgaagat ggtgaaaggg tcatgcaatg aaacgtattt
    42121 ctttgctgtc ttttgtgctg tgtcttctgt ccgttccatg ccctctgtcc gctgccgccc
    42181 gggaaccggc accctccatc ctgctggacg aaagctacgg ggcgcttctg gaaagcagta
    42241 gtgatacgct tgctttgtgg tgggcttctt caggctggaa agtatcgcgg gagcgcggat
    42301 tgcccgaaga aaaggcgagc tctatctctc ttgaactggc gcgaaacgaa tgggaagcca
    42361 cccagcttgt actgcgtccc cacaaagcca tgtccgggct tcatctgagt gctacggatc
    42421 tgcaaagtgc ttccggtgcc cggattccgg cggattgcat cgaactgctc cgggtacgtt
    42481 atcttgctgt ggaaaaggct tctgacgggt tcgggtgtga agggttctgg cctgatccgc
    42541 tgccgcctct tgaaaattct tttgacctgg aggcggaaat caatcagccg atatggatac
    42601 gtgtttatgt gcccggagga tcagaggcag gcaagtatga aggtgcgctt ctgcttgaag
    42661 gtgaaggaac atccgtccgg attccgctgc agctgcgtgt ttacggtttt accctgcccg
    42721 acagaagcag ctgcacaagc gcattcggtt tcagccctgc ctgggtctac cagtaccacg
    42781 gactggacaa gcaggaagac cgcaaagcgg tgctggatct ttactgggag agtttccgga
    42841 agcaccgcat ttccccctat actcctgccc cggaagttgc accggaagta aactgggtac
    42901 cgctggagcg tgatgcctgt caacatgtca gtgatgaaga tgcgcagctt ctgacagagc
    42961 atccgctgac ccctgtattt gattggtcag cctgggatca ggagatggat cgcgtcttca
    43021 aaactttcca ttttaattct ttccgttttg gtgtgcccgg cattgctgct gaaagctatc
    43081 agggctttcc ggaaggatcc aggggatatg acctcgcatt caatgcctgg ggcgctgccg
    43141 tggaatctca tctgcgggag acaggcaggc tgaatgacgc ttatatttac tgggttgatg
    43201 agcctaccga aaacgactac cctcaggtga tgaaaggctt taatcagctg aaaaaagcac
    43261 ttccgggcat gcgccgcatg ctgactgagc aggtggaacc ggaactggca ggcggtcccg
    43321 atatctggtg tcccctgctg tccctgtaca accatgagcg cgcggaagag cgccgagccg
    43381 caggagacag cttctggtgg tatatctgca cgggccccaa gcagccattt ctcggccttt
    43441 tcatagatca tcccggcaca gacctgcgtg cctggctctg gctgacctgg aaatacggga
    43501 tcgaagggat cctgatttgg cataccaact ggtggacaag caacaccgct tatcctgacc
    43561 agatgcagaa tccctacaaa gaccctatgt cctggcagac gggatacggt gccaaaacag
    43621 gcgataagaa gccctgggga aatggtgacg gcaggttttt gtacccgcct gaacgcgcgg
    43681 cggacggaca gccggagcag ccagtactgg aaggtcctgt ggacagtata cgctgggaac
    43741 tgcttcgcga cggcgtagag gattacgaat actttgtgat tctgcgccgc ctgcttgcgc
    43801 aaaaaagcga caatctggac gaaaaagagc gccaactttt caatgaactg ctgctggttc
    43861 ctgaaaaagt ggcatccggc ccgaaagaat acacgaaaga tccggcgccg ctggagagac
    43921 agcgccatcg cctgggcaga gcaatcgaaa ggcttcaggc gctggaaagc aaataagagc
    43981 cggaacaatc gcgcttctcg ttttgttcca cccataggcc tttgaaagtg aaccattgtt
    44041 ttgtcccgcc tctgcagggg tgataccata tgtccgctat gcgagtattt acttacgaca
    44101 gtttagaaga gcggagaccg cggcatatcc tgatggatct gatccgggcg cggcagctgc
    44161 tgcttgatct tatctggcgc gacttgcggg ttcgctaccg ctatgccgtc atgggctttc
    44221 tgtgggcagt acttgagcct cttgctttta cggttattct gacactggtt ttcacgctgg
    44281 tgtttgcacc ccgcgtgccg gatgcggcgc aaacagccgg accgccttat gtggtcatgc
    44341 tgctgtgcgg cattattttc tggcagtatt tcagtacatc tgtcactact gccaccacat
    44401 cggtggtggc aaaccagaat ctggtcaaga aggtgcgttt cacccgcgaa atcattccca
    44461 tggctgcatg ctgcgttccg ctggtacagc tgggcatcgg tctgacgctc ctctttatcg
    44521 tgcacctctt tctgggggga gccctgagca gtgcgctgat ctggcttcct tttgtttttg
    44581 caatccaatt tgtgctgact ctgggactgg ctcttctttt ttcatgcgtc cacgtccatt
    44641 tccgggacgt cagcaatgtg gtaacggtgg ggctgcttct gggattttac gcctccccga
    44701 ttttctatcc gctggaaatg gtacgcgaga gcaggctgcc gcaatggctg cagcagctct
    44761 acctgctgaa ccccatggcg gggctgctca ccgcataccg ccagattctg ctggaatatc
    44821 gcttcccgga ctggcagctg ctgatagcac cttccgtaat cgctgtctcc gcctttatcg
    44881 caggattgct gctcttccgc cgcatcgctg ccaccatgtc agactacctg taatctttat
    44941 tgaaaatttc cgactcagga acgaaaacga atggtcagaa tagaaaaaac aaaaaagcgt
    45001 gtctataccc ggaggggtgt ggtctggctc ggtcagacat gcaaccagcg ctgctatttc
    45061 tgttatttca tcagccgtat tgaagatcac tgccacccgc tccacgcttt catggagctg
    45121 gataaggcaa aagagatcat gaacaggctg cgctatttct acggcaaccg gtctgtggat
    45181 atccagggcg gagagcctac cattttcaag gagatcctgc cgctgatatc gcattgccgg
    45241 gatatcgggc tgtacccgac gctgatcaca aacgggctgc agctggcgaa gcccggcagg
    45301 gcagaaagtt tccgcgaggc aggcatccgc gattttctgg taagtctcca cggctacggc
    45361 aagcagcatg acgaagtggt gggtgtgccg ggcgctttcg aaaaaatctc tcaggctctt
    45421 gacaatatcc gggaagcaga tataccgatc cggatcaact cgaccatgag caaaccggta
    45481 atccccgtaa tcacgcaagt ggcgcaaaag gccattgacg ccggtgctct cgtggtgaat
    45541 tacatcgcct tcaatccttt tgaagaccag catacaggaa ggcggcgcca cgatactgtt
    45601 gcccgctact cggagatcat gccccgttta acggaagcca tcgatatgct ggaggaagcg
    45661 ggggtggaag tgaatgtccg ttatctgccc atgtgtctgg cggaaaaacg gcaccgcaaa
    45721 aactttttca attaccagca gctttctttt gatcttcacg aatgggatta ccagagttgg
    45781 ctctggacga tgatgatgcc tcagatgatg aaggaaggcg ggctcattcc gcccttccgg
    45841 ctgggtactg gtgcacgccg gctttataag gcggatcccc tttatcttcg ggacaatgca
    45901 gccaaacatc ccgtcaagac cggatttaaa tacgccgtgc aacgcgctgt tgcgaaagtg
    45961 cagcagacaa tcaacaaaga cgggatttat cgtgaagagg caaagcagcg cgccactgtg
    46021 gactgcggct acagacaggt tcccgcctgt gcaagctgcc accttcgcca tatctgtgac
    46081 ggatttcacg gcgattatac a
//
LOCUS       DAOPWB010000007        45153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_763_length_45153_cov_5.508626, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000007 DAOPWB010000000
VERSION     DAOPWB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 45153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45153
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_763_length_45153_cov_5.508626"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="PKN92_01535"
     CDS             complement(<1..1726)
                     /locus_tag="PKN92_01535"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNZ47504.1"
                     /translation="MRFFSVIFCLSLALTATAADFSVLPANPDGTPHAAMMYKYLLHE
                     AEGCLNARMEAYEALKTEADILAWQENLRSRFTESLGGFPERCPLNARITGQGEKEHF
                     RYEKVIFESQPGLLVTAILYLPKGQGPWPGVIVPCGHAAEGKGMDAYQRASMLLAANG
                     TAALCYDPIGQGERCTFLKEDGSVELGSVIDHTLVGAKAILTGMNTASFRVWDGMRAI
                     DYLQSRDDIIADKIGCTGNSGGGTLTSYLMALDPRIVCAAPSCYLTSFDRLLNTIGPQ
                     DAEQNVFGQISYGFDHADYLHLHMPNPVLMCVATRDFFDINGTWSTFRQAKRLYTRLG
                     VAERIDLIENDEEHGFHKAQREGMVRWMSRWLLQQDRFITEPDFDIFTEKEFLCTDRG
                     QVIYEAGARSILDLLSERENMLAQKRTAFNRSADPSAIREKIKELIALRTSDTAAPEA
                     LVLKEDTYKGIPLQYLALNPEPGIVLPALLAMPEAFTGSWILICSAEGKASLFGNPER
                     IQALLKEGNAVLALDLRGLGESANHEGGLGWHKTVGPDWQDFFLAFLLMRGCDCMLTA
                     HILTAAPWL"
     gene            complement(1798..3960)
                     /locus_tag="PKN92_01540"
     CDS             complement(1798..3960)
                     /locus_tag="PKN92_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47505.1"
                     /translation="MMTFVRCSLALLLFALLFGLAAEAEGPDRTCFQEAGHWQPAIDL
                     GADMAIVYGVNDSFADRVRQWREKGYSIGMMTGIAWGGYDDYYRTADGFKKDEVQTRK
                     SGKLYMHGDSTTVGYNVPTDAYIDYIKQVVNPAVDLGAQAIFLEEPEYWAEAGWSEAF
                     KEEWLRFYGEPWQEPDSSPDAQYRASKLKYELYFKALREVFRHIKERAAAQGREVECH
                     VPTHSLNSYAQWRIVSPMSHLMDLEEMDGYIAQVWTGTARTPNLYRGVQKERTFESGF
                     LEYSQMVAMVRPTGRKLWFLADPIEDNPNYSWRNYKENYECTVISSLLWPENYRYEIM
                     PWPTRIFKGSYPKVDLDTKSDDREGIPADYAAELMAVTNALNHMDQDKVQYDTGTQGI
                     GVLVSDTLMFQRAAPRGSDALLSNLYGIAMPLVKHGIPARFVQMETLTQPGALDGIHL
                     LLMTYDGQKPLKPDYHEVLDQWVRGGGALLFVDDGSDPYNRVREWWNDEGKNDALPQD
                     DLFRRLGISSEQADTPVAVDGGRVYVMNISPTRLAKQTEGAERLMQAVALMLQQKGIT
                     LQTRNYMKLRRGPFLIASVMDESISEQPLSLQGSFIDLLDAALPVVKEKTLEPGQRCL
                     LFDLDWAAAHHATAEVAAASCRVREESMNGSTFSFVTRGPENVRARMRIRLPKAPETI
                     TVAPDDTEFLSAWDSSSGTLMLECDNQAKDIRFSLQLQ"
     gene            complement(4024..5481)
                     /gene="tilS"
                     /locus_tag="PKN92_01545"
     CDS             complement(4024..5481)
                     /gene="tilS"
                     /locus_tag="PKN92_01545"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNZ47506.1"
                     /translation="MTLSSIESKLMTSIRAVLAEAGALSKNRLILAAVSGGQDSMVLL
                     HLLNRLGVPLIAAHFDHGTRSGESALDAKLVEDFCVEKGIPFRSARESLEEDARRTGE
                     NFEALARRKRYAYLTDTAHREGCEVIATGHHLNDQVETILMGSLGLASSFGGSGMEPV
                     CRKNGFLLVRPMLHIRKEDIDACARSAGVPWREDSSNPDQRYARNRIRSILLPVIDSL
                     GSHCKAQLAERAALQREALHYLDEEGGRVLKQCGRAGHQRNILLYLEYEKLCSYPPLL
                     VRHALRVLARLLKVVFSRADINRIHRLLMDSKPGKEYDLVEGLILYKRGKECMIFRNL
                     PALEDEWPTVPTPGEIIYSHFSVQTSFPEPLHVMHLIEYAASQGIFYFYEWQVEYPLY
                     IRTWDGIGWMVPFGSSKAKPINKILRERGIPPTLRRLECIICDELQRDLLFSSGHRTD
                     MAVLSEHHSNSILAIKITPLDTSDPDIVAYFDSSD"
     gene            complement(5478..7373)
                     /locus_tag="PKN92_01550"
     CDS             complement(5478..7373)
                     /locus_tag="PKN92_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47507.1"
                     /translation="MKRDQRQQKHDEVFSEHTTFFPEDATETGLCLLALTLPLGIGAR
                     VAGPLYLHHPALLLLLFLLGRDWQRIKRLTVPFELIFPATLLCLLSLLLIPGGSAAVA
                     GKIIAFSLFFLLIYHSTRRSAVVWRALQLSLWSSSLVILLSLPAQHGLLFPTAFQPGN
                     SFVWLGPSNFRSGPFHLIAVLLLPFLLLTARMTGMHRSRHSVALLETLPVLLPGIVLY
                     QSGTFPASGTDSGTAFLFPALPLTLTALWLAARTAAKLQVARSHMPQALAALFALSVL
                     TGTVFFLLTDNMPDGYAVFLFALLAACAKPRLKWNETAPHGTVLLILPAALLLIFNLT
                     FIRSGDPRHYGHTAEQALHSRDYAALQAQLDFVQNRCPGERYADFCEARLHLALGNLD
                     TARHCFAQSLLPGRNRLLPPPGTQEITRFLDDMRDASSALPESLRGLAYEQALLAAGR
                     ERHALSLLEIRNPVETHTTLNPLPAAAALAQLLERPELEEKFRRWDGALLAAILKSAH
                     PLNELRVAPPGFPENRLPLIACARIEGNRVELSLFSPAREEGASYINPAATEIFSPEH
                     SFPDFWGELYQNGSGAWRLPFSVCAELAPLDEAPFSFYPDAMPSDPETEGITQLVIYI
                     PGSGETP"
     gene            complement(7370..7576)
                     /locus_tag="PKN92_01555"
     CDS             complement(7370..7576)
                     /locus_tag="PKN92_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47508.1"
                     /translation="MKTPQAATLQERPAFLPPLERMRRERRLTLLFFTGFMLLAALFH
                     LLASEHSPAEAPAVPHAVTEEHLP"
     gene            complement(7655..10585)
                     /locus_tag="PKN92_01560"
     CDS             complement(7655..10585)
                     /locus_tag="PKN92_01560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNZ47509.1"
                     /translation="MKTSVLKITGLLFVCVLAAAAADSNENAGKGFESMGGPYPDDPR
                     HVHHYRLDNGLEVFLSACVDEPRFYAEIAVRAGSKNDPPDATGLAHYMEHLLFKGSEK
                     LGTLDYEKEKPHLERVSELYEQHFNATDPAERKAIYQQITEESTRAAAYSVPNELDRT
                     YSALGARNLNAHTWVEEVVYKVDLPKNCLEQWAMIESDRFLHPVFRLFQTELETVYEE
                     MNRAQDNKNRIISEAVAACAFKNHPYGQQTTLGKVEHLKSPSIRKLEEFYRTWYVPNN
                     MAIFIAGDIDPDETMEIINRYFSSWEPRPIPEQKQWQEDPLRGREQVTVSYEGEEFVL
                     LGFRTVPFLHPDRLPLLMLDMMLDNSEAGLINLNLNKNQRVQSAGAYPMIFNDYGMQY
                     LYGTPKEGQSKEEVEALLLEQLRIIRAGEFDDWLIPAINNYFQKSEKAKLEKYSSCVP
                     LMRDAWLAYEPWEDAYRFLDQMAAVTREDVIRVANEYFGENYVAGYREDGSHNPPLVE
                     KPPLPDINIDAAAQSDLAKAVLALPQPDVAPVFLQENRDYRKLQDERGITLYHTPNPY
                     NDLFSLTFSFPLGTRQDKRLAAATNLFSKTGTETKSPDELQIEWFKRGSSFNISASAN
                     DVVLIILGLDDQLPATLALLQEMLEHPAVTEESLETMKAVTLKIQADLRKDPGGLMTA
                     LSSYMAYGEESAARQWLPADQLKALTAAELQELLRSLFTYKYHVLYSGTMTPEEALRL
                     TGSLCRSDAPLTDRLPYYQPTMRTPEKNEIFFTQREMGQAHITIRYPGVNYDPELMAQ
                     VNLFNEYFSGGMSGLVFQELREVRALAYAAYAAYQPGARRTEQNTMEGMIQTQADKAP
                     EAINAFIDLLDNMPLSAERFKQARETLLKDFAFTRTSFREIPALALSWERKQIEPDPR
                     EKVYQKIQQMTSIDILATFHKEHIAGKAKLISVLGDTARLDFNALGGIAEVRTLTPDE
                     LFIN"
     gene            complement(10759..13557)
                     /locus_tag="PKN92_01565"
     CDS             complement(10759..13557)
                     /locus_tag="PKN92_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ47510.1"
                     /translation="MSKKYVIHVISNTHWDREWLNNFQETRMMLVEFFDLLLEVMEGD
                     DSYTSYVLDSQTVPLEDYLEFRPENRERLAALIEAERLLVGPWYTCPEGFEVNGESLV
                     RNLLMGHRVASGFGHVMKVGHTPFSYGQNSQMPQIYSGFGIDTMLFYHGVSHQEVSNE
                     WIFEGADGTRILGSQMSSGARYNFYHGVYRPAVIGRTVAEREHEWKEGGMPFHRASPE
                     LSLTHHLLLQPRRTIDKTRLEQAVRALKEAETGVSVTRHLCFMMGHDSSVPDPLEPEL
                     IRLTREALPDDEVVHSNYESMLRAVREEIDWNKLTVLKGERRVPKPMPIILHLYSDVL
                     SSRTRMKKRSSRAEYKLQRTVEPFLMAAALMGAPWPAAMLEYAWKTMLRCHAHDSISG
                     SGVDAIEEDMMNRLRQVLDLSDSLGSRALAHMQEQIDTSALPSDAVVLTVYNPAPRER
                     SEVVTAVVDLPWQGPRPRGQFTLRDAESGKAIPVQCAARKPHWAVMNHAFDAPNLMKS
                     ERFTIHFEATQLPGLGYATFVADLSGSFDTGTLVTAANTLENEHLRAEIRPDGTITLL
                     DKHTGVSYPDLHYFVDDGEGGHAWMHVRPAYDRPVDSRGFPVVISLEEDGPLLARYRI
                     EYRMSVPSGIDDNGGDPWQRLDGVGNHASRGTMEEEMTIVSRITLRRNSRSLEVQTSF
                     DNRAQCHRLRVMLPTHRKGKYCHAESAFDVVERETAFDENSVWHGAKGVTFPMQRFAD
                     VSDGTAGIAFISESLREYEVTDTEDRAIAVTLLRAYEINLATVSCRWETHPEMILSQS
                     PGHHEFTYRIHPHAGNYAEGAVLEEADDLVAPLEPVQAGVCKGRAPLRQGFLTVSPAS
                     LLLSAVKQAEDGAGWVIRLFNPTERTIEGSVTPPPSVTGAALLTLEEKRQETLSLEAG
                     QLHLSVAPKKIVTLLLEN"
     gene            complement(13667..15103)
                     /gene="ahcY"
                     /locus_tag="PKN92_01570"
     CDS             complement(13667..15103)
                     /gene="ahcY"
                     /locus_tag="PKN92_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNZ47511.1"
                     /translation="MPELTDTLPFKVADMGLAEWGRKEIEIAEQEMPGLMAVRRQYAE
                     AQPLKGARIMGSLHMTIQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAIAATGVPV
                     FAWKRETLEEYWWCTWQAMNYPDGKTLNMIVDDGGDATLLIHRGCDFEKSFEETGALP
                     PVCRDNKEIEVVDSLLYKVHEMDPGYWRRIAKNWIGVSEETTTGVHRLYHMMREGTLL
                     TRAMNVNDSVTKSKFDNLYGCRESLADGIKRATDVMVAGKVVVVAGYGDVGKGCADAM
                     RGLGARVIITEIDPICALQAAMEGYQVLTMDEAAPLGDIFVTATGCCDVIAGRHMEQM
                     KDQAIICNIGHFDSEIEVSWLESNPSISEENIKPQVDKFIFPDGRAIFLLARGRLVNL
                     GCATGHPSFVMSNSFTNQTLAQIAFFTEPDKYEAGKVYTLPKKLDEEVARLHLGKLGV
                     KLEVLTARQASYLGVPVDGPYKPDTYRY"
     gene            complement(15266..15460)
                     /locus_tag="PKN92_01575"
     CDS             complement(15266..15460)
                     /locus_tag="PKN92_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47512.1"
                     /translation="MPVYEYHAIRAEGDEEKSFIGGIVVAKDRREAKGKLREHGLKAT
                     QLKRARGIMAVIKWLDADIR"
     gene            15783..16466
                     /locus_tag="PKN92_01580"
     CDS             15783..16466
                     /locus_tag="PKN92_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ47513.1"
                     /translation="MPQNTAIVIPCYNAGNRLESVLNGLTSFEGPVIVVDDGSTDGGT
                     DCLSERTVQALRFPENRGKGHALLAGIREALQHEAVQRIVLMDADGQHDPGEVDALLS
                     ASRSTGAALVVGARQIDRRLMPLRSRFGNVLTAWISAHVFRCPLSDTQCGFRVLSRAF
                     AESFLAQSRGGRYETEMEMILFALREGCVLASAPVATVYEPGNRSSHFRKIRDSFRIY
                     RVLLRAWRR"
     gene            complement(16477..17577)
                     /gene="aroC"
                     /locus_tag="PKN92_01585"
     CDS             complement(16477..17577)
                     /gene="aroC"
                     /locus_tag="PKN92_01585"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNZ47514.1"
                     /translation="MRRHLANTYGEVIRATTFGESHGRAIGVVLDGVRPGIPFDEEDI
                     QKELDRRRPGQSKVVTQRREADTVEILSGVFEGQTTGAPLAMVIRNEDQRSKSYDNLK
                     DLFRPGHGDFTFYKKYGVRDHRGGGRQSGRETACRVAAGAFARSVLESMNIRIVAHSI
                     EIGGIQASKCDLSIIETNPVRCADPDAAPLMEEAILKARSEKDSLGGIIQLEVHNLPP
                     GLGDPVFGKLDARLCSAIMTIGAIKGVEVGDGFAITRLRGSQANDPMGEQGFLSNHHG
                     GILGGISSGAPLIMRIAVKPTASIASRQRSIRISGEPCDVEVKGRHDPCIVVRAVPVV
                     ENMAAWVLLDAFEVQARINPEWAEKYYPPAAP"
     gene            complement(17611..18078)
                     /locus_tag="PKN92_01590"
                     /pseudo
     CDS             complement(17611..18078)
                     /locus_tag="PKN92_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            complement(18107..19396)
                     /gene="aroA"
                     /locus_tag="PKN92_01595"
     CDS             complement(18107..19396)
                     /gene="aroA"
                     /locus_tag="PKN92_01595"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNZ47515.1"
                     /translation="MEVTIRPSTLRGRIQIPASKSHTIRAVVIAALAPGESCILNPLE
                     SEDARSARNVYNAFGAQITEEPEMWRIVGTNGRPQAPDNIVDTGNSGTTMRMALGSAA
                     LIPKGLTVLTGDDQVRRRPGGPLAQALNALGAQVQSTRGNGCPPWVVGGMLRGGETEM
                     EAVTSQYVSSLLLNAPLAEGDTRLHVPLLNEKPYVVMTLDWLKNQGIAVQYSDDLSEY
                     HIPGGQQYLPVNRPIPGDFSSATFFLAAGALPGNRVACAGLDMNDSQGDKAVVSFVKE
                     MGAVLRCEKDEVTVAAKKLRGCEFDLNATPDALPMMAALACLAEGTTRLVNVPQARLK
                     ETDRIRVMRMELEKLGAAIEELEDGMIIQGGVLHEGVVDGHGDHRIIMALATAATAAT
                     GPVTIRGTEAVTVTYPEFFRHLQALGGSLSGSGIEAS"
     gene            complement(19450..20349)
                     /locus_tag="PKN92_01600"
     CDS             complement(19450..20349)
                     /locus_tag="PKN92_01600"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNZ47516.1"
                     /translation="MTMKIKIRGIMAAMVTPFTKGGENVDYDKIGPLANRLLSQGAHG
                     LFPCGTTSEGMLMSAEERKETLEEVLRTAPKKTPVIAHTGCLDYATTLELTRHARDAG
                     ATAASIVAPGYYAYDDAGLFQYYRNLARAVDGFPILLYNIPACARNVLSPELVLKLAE
                     SEENIVGIKDSSGVMTALTRMIGNAPDGFHVICGTDDYGFQAILAGCPALVSGLSNVV
                     CEIYAAVYENIQKKNLKKAWQEQIRLERAARIFKYGQNLASFKEGLRLRGFDAGYVRP
                     PQRDLTAAEKKQLEKDMQELGVI"
     gene            complement(20437..20934)
                     /gene="ruvC"
                     /locus_tag="PKN92_01605"
     CDS             complement(20437..20934)
                     /gene="ruvC"
                     /locus_tag="PKN92_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNZ47517.1"
                     /translation="MLVIGFDPGSALTGFGVVSRQGNRLHHREHGTIRTPAKQHSAQR
                     LQTLFQEAEALIARIQPDAVAIERLFFKQNVTTGIQVAQARGVLLLAAAQAGCPIGEF
                     SPTEMKIAVTGYGRADKKQLQEMVRRLLNLPEVPRPDDAADALALAICQIQFGATQER
                     LQAEP"
     gene            complement(20935..21687)
                     /locus_tag="PKN92_01610"
     CDS             complement(20935..21687)
                     /locus_tag="PKN92_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ47518.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGKIFSKLAKEITVAARLGGGDPA
                     ANPRLRSVLLAARTQNMPGDNVDRAIKKGTGELPGVTYDEVRYEAYAPGGVALIIDVL
                     TDNKNRTVSEIRHLVSKLGGNMAEAGAVAWNFEPQGLLTIPKEGISEEEMFDKAIEAG
                     AEDVSGDGDAYEIRTAPTQLHVVAGALEAAGTKAEEVKLTMMPKTTMELTGKQLLSCL
                     RMIEELEDHDDVQDVFSNLNITDEAMAEVMDE"
     gene            21974..24286
                     /locus_tag="PKN92_01615"
     CDS             21974..24286
                     /locus_tag="PKN92_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ47519.1"
                     /translation="MYESFYGLRERPFNLTPDPKFLYLSEKHKEAFAHLLYGIKNRSG
                     FVMITGEIGTGKTTLCRNLLNQLDSNTEVAFIFNPFLSAIELMKKINQEFGIESDADT
                     VLELTEELNVHLLHSSAMGKDCVLVIDEAQNLSPQVLEQIRLLSNLETDTEKLLQIIL
                     IGQPELAEKLALRELRQLNQRITARYHLKSLSASETLQYIAYRIHVAGGRKRINFTKA
                     AVTQVYKLAGGVPRMINALCDRTLLIGYTKEQHVISAKLVKQAEKEIRGEKITAPKEP
                     RTRWRRLIPSPALLLILAVAIVLIHYLVSPLDRFAQELNHFNRILTGSTEAVPGTPES
                     GAARTAAEAASDATSPAVPVQPATEEPLRQVLNRLNAAGAGKNAQQLAPVLAELDAEK
                     ALQGGLETLLSLWNRPSPSEYPAKGDADTLVRFLEAQGLASEHIRPSAEHLAAINLPG
                     LVRLHAGEITCWLGLVRMDQETVTLLPAVNKRIIVPLKEFSLYYAKEAVFSWNDAGFS
                     GPVLVLGQKGPQIEALKEKLRRAGLLDAGNTGDTFDKATEKAVMRIQELAGLKADGKA
                     GRQVRLALASFSADVPTLQERPAAGNAAESEGGAPADAAAITAAETVSKVPAPAKQDP
                     LKKAVVAEKTAPVRKPSGETPAQKTAAPAAEKEIIFTAAEPSAASESNTDSSLSEERD
                     AAAPGQESSLLRLLGAPADGVREEEEKVSAPSVLSVGGEKLIEERELPEPGVPFIAPG
                     YAPEGLPSSATMPVTGSSPLVPAARESAYQ"
     gene            24327..25460
                     /locus_tag="PKN92_01620"
     CDS             24327..25460
                     /locus_tag="PKN92_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47520.1"
                     /translation="MSSILDALNKLEQEKAAKQSQPGDYEPTLSPEEAASVLLGESSV
                     PEREKRMPVWLPVVLALCGGFVLALVPVGYFLAVKGGATPPEQVAGAPAADTHSYQMP
                     VTAPPPVAAPVPEEKKAASKAEPAGETPPTPPPAPPKSAPAAVPEKKETTRPPLPESI
                     PLPDRFPQETSAPPPVVKVADLPPPVPEPAPVPEPVAPVPPPAPAPVSEPAPVPVPEP
                     VAPVPPPASVPAPEPVAPAPPPAPVPVQEPVVVPVPAPVPAPAERSVIAQTPSEYPSA
                     SAGTVPRVPSARSSGGAVSVDNLPRLSSQERARFGLDGLRVNVLRPADKDQPDALAII
                     NLKKVYVGETIPGTRARLIGVASSGIGIEIEVEGSNKQFRIPR"
     gene            25488..26465
                     /locus_tag="PKN92_01625"
     CDS             25488..26465
                     /locus_tag="PKN92_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ47521.1"
                     /translation="MRYITTGAVLLSLICLAGCGGSGSGSSGSGGAKSGALEIAVIPK
                     GLSHQFWLGVKAGAEAAAAECGASVVWQGPPKETEIAQQINIVQDMITRGVSAIVMAA
                     CDENALGAVIAQADRAGIPVVMVDSGVKSDIPKSLVATDNLEGAKMAARVLADLMGGQ
                     GELGLLPFISGAATSEIRERGFKEGVTEFPGMKIVATNYTQSDVSTGMNVTSDMLTAH
                     PDLGGIFATNEPGAIGAAQALRAAGKAGKVKLVGFDATEEEINALKEGVIQALIVQDP
                     FKMGYEGVKAAVASLKGEEVPPIIDTGVTVVTLENLESPEVQKLLNTGK"
     gene            26620..27999
                     /locus_tag="PKN92_01630"
     CDS             26620..27999
                     /locus_tag="PKN92_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNZ47522.1"
                     /translation="MDQPFSKDMLPPESTEIILESISDGVFTVDHGWRITSFNRAAEE
                     ITGIPREEALGRRCCEVFRASTCEEECALRHTLDSGEPIINKSVYIVDAAGERIPISL
                     STALLKDERGNVVGGVETFRDLSLVEELRREIEHRYDLGDMVSRSAAMEKLFQMLPQV
                     AASEVPVVIEGETGTGKELLARAIHDLSGRHKGPFLGINCGALPDTLLESELFGYQAG
                     AFTGADKDKPGRFALAEKGSLFLDEIGDISPAMQVRLLRVLQEKQYEPLGATRAFHTD
                     VRIIAATNKSLGALVQEGLFRQDLYYRINVIRLELPPLRERREDIPLLVDRFIRRNNH
                     LRQNKTVEGVTAEAMRLLMSYDYPGNIRELENSIEHAFVLCDRPLIDVGCLPEAMQAF
                     YRRSGAGTAPSLNNAVQEAEARAILDALKACDGNRQKAADLLGMHKSTLFRKVRALGL
                     TLPPATRRK"
     gene            28164..28670
                     /locus_tag="PKN92_01635"
     CDS             28164..28670
                     /locus_tag="PKN92_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ47523.1"
                     /translation="MKIALTVREQRISPVLDTARTLLVVELQQGVVVSRQEKEIAGGT
                     VQNLLSQLEALGVRQLVCGAVSRRFADWLSAAGIQLIPFISGDVEEILAAVLAGTVCS
                     GAFAMPGCGSGGRRCGAGKGQGYGRGKSGFSGGHSVCPECGCRLPNCRRHPESAVCPG
                     CNRPFGHE"
     gene            28677..29363
                     /locus_tag="PKN92_01640"
     CDS             28677..29363
                     /locus_tag="PKN92_01640"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNZ47524.1"
                     /translation="MMTVSTQEATDKQKTPWHGRGLTSFLISLVFLTLAVSGLVLYLA
                     PSCRIARETGWRVLLLSKDQWNALHIIMACTFVVAGLIHVYFNWRILLRYLFLKQRLH
                     LKKEAVTALLLFLLLSAGAIFEVPPFSFLTALTEEEKAGSCAGVLSAEAAHPASGHGT
                     GVGRMTVQEFCDDAGLLLEDTLSRLQEAGFTADPGSTFRALSQQKQMSPMDVKEWLLN
                     RGSLDKEERN"
     gene            29367..29651
                     /locus_tag="PKN92_01645"
     CDS             29367..29651
                     /locus_tag="PKN92_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNZ47525.1"
                     /translation="MPGRDGTGPCGKGALTGRGAGGCLRDGRGMAARRGAGSGRGMRG
                     GMGFGRGFGRGFGLSSETESQSLREQAARLQGALEEVQDRLASLEKSSDR"
     gene            29701..30063
                     /locus_tag="PKN92_01650"
     CDS             29701..30063
                     /locus_tag="PKN92_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ47526.1"
                     /translation="MKIAFSTSGKDLNALVCSRFGRADGFLIYDTDTDEYTVVENQQN
                     VNAPQGAGIQAAQAVVKAGASALVTGHCGPKAFTVFAASSIRVYHSDEVPISEALQAW
                     RRDALAEMHAADVDGHWV"
     gene            30283..31152
                     /locus_tag="PKN92_01655"
     CDS             30283..31152
                     /locus_tag="PKN92_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ47527.1"
                     /translation="MHIAIASGKGGTGKTTVAANLAALTEGDETVYVDCDVEAPNGHL
                     FLKPELSFSETAGIPVPQVDPALCTGCGKCVEVCRFNALACVAGKLIVFAELCHGCGG
                     CVPACPEKALTESSHAIGTVSRGMAGDLHFVQGTLRVGAAMSPPLIRAVKAKAPDAAV
                     IIYDAPPGTSCPVITTLKGMDYVVLVTEPTPFCLNDLQLAVETVRTLGLPFGVVINRA
                     DVGDARVRDYCDAEDIPVLLTLPEDRRIAAAYSGGALIVDALPEYRASFMELLGKIRD
                     GAGQREKGKAVRS"
     gene            31149..32102
                     /locus_tag="PKN92_01660"
     CDS             31149..32102
                     /locus_tag="PKN92_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ47528.1"
                     /translation="MTSRKHSFIFGPVVSRRLGRSLGVDLIPAKTCPYDCIYCEQGRT
                     TCHTMQRDAYVDVEAVLEEVAAFLATHPAPDYITISGAGEPALHTGLGELIQKIKSLT
                     SVPVAVITNGALFWQQEVRDALLHADVVLPSLDAGNERLFQKIDRPVAGLSFAKMLEG
                     LIAFRNEFSGALWLEVFLMDGINDDEDSVREIAAQVRLIRPDKVQINTVERPPAMSRV
                     KAVSFSVMERYVSFFEPEAEIISPRYIPDSGGVLLPQEAPVLEMLRRRPCPLEEIAEG
                     LGFHRLETAKLLDRLVKEGRLCVMERENKLFFGIGERYEQA"
     gene            32089..33027
                     /locus_tag="PKN92_01665"
     CDS             32089..33027
                     /locus_tag="PKN92_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ47529.1"
                     /translation="MSRHDIRELVIISGKGGTGKTSLTAAFASLAGRAVLADCDVDAA
                     DLHLLLHPEPEEHYEFRSGHQAVIRQEDCTRCGDCYERCRFEGIRREDTGADVRYTVD
                     PLSCEGCGVCVDWCPAAAIDFPECTAGEWYVSSTPCGPMVHAKLGIAAENSGKLVSTV
                     RRAARDRAEKEGIAKLIIDGPPGIGCPVIASITGTSLALIITEPTLSGQHDLERVARL
                     AKHFNVPVAVCVNKWDINSELTESIEAVALSMGARPVGRIGYDRRMTEAQVAGKTVLD
                     YGGAAAEAIREIWCRVASMLDGTAPDAGDAESGNGG"
     gene            33057..33455
                     /locus_tag="PKN92_01670"
     CDS             33057..33455
                     /locus_tag="PKN92_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNZ47530.1"
                     /translation="MEENYSAKALERFRNPRNYDELQEPDGYARITGPCGDTMEFWAQ
                     VDDGIVSAASFTTTGCGPSRACGSMATELAEGRPVAEAKEISQQDILNALGGMPEAHR
                     HCALLASNTLRAALENYQASASVKQMKTEA"
     gene            33514..33855
                     /locus_tag="PKN92_01675"
     CDS             33514..33855
                     /locus_tag="PKN92_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNZ47531.1"
                     /translation="MMRVAIPVVQGHLALHFGHCAQFALVDTDPEQKSILKVDVVDAP
                     EHQPGLLPRWLAERNAQVIIAGGMGARAQALFAENNIEVVIGAPADPVEALVQRYLEG
                     TLESGENVCDH"
     gene            complement(33879..34319)
                     /locus_tag="PKN92_01680"
     CDS             complement(33879..34319)
                     /locus_tag="PKN92_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HNZ47532.1"
                     /translation="MNSESAKQRFQEANRLFKNRQYEKALELLQQLNREFPNTENIMY
                     AAALSLEKLGSIDEAKYLCHSMIRNFESVRAQELLNHLEVAPRVAAPQGDIPDAAVEV
                     LPKHARLEDIGNPSVKRRNRMLVVAVVVLAALVLALPFLLKMLR"
     gene            complement(34396..35058)
                     /locus_tag="PKN92_01685"
     CDS             complement(34396..35058)
                     /locus_tag="PKN92_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47533.1"
                     /translation="MKPIFCFSMLLCSLFFFGTALADEAPDNDLDRMLHGVRLNMSFE
                     ECLKRLPDAVYSDAASRNTTPGPQKPDALLIAYGADPFLGVHAFANIGFQDARIYELV
                     AVWSDDAKDIEARCRRFFKALDERHGTAYEKKSLFVFPGSDQQQAVAVRVWQEADTVT
                     LAFYTPPGENAPSQKASLSFAQFKTGASFLDDVFVNNPVPEEQATEAWEKMEKPNATP
                     LP"
     gene            complement(35077..35985)
                     /locus_tag="PKN92_01690"
     CDS             complement(35077..35985)
                     /locus_tag="PKN92_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ47534.1"
                     /translation="MALFRIAVILFIPFLLIGALLFWNNYAPKEAERYDRTLPRNPDT
                     GILSGAEARTCGPDDTRKAILFVHGFIATPDCFSDLPEYVAERGWHVRAMLLPGHGTS
                     PFDFQKTSPAALEEAVLKELDALQQRYDTVVILGHSMGAALATIAAAQKQPDGLILTA
                     PYYAVTKQWYYILRPETWAGLVSPILHWVYRPQNMQPVNRAEARGEILSYNWIPSKGA
                     LTAMAVAGKARDDTILQKIAMPTLLIHSKNDTVADPAYAEAVYNRIPSKDKTLHWLLL
                     SDHILFYDYDRETVKIQIAAFLKKWD"
     gene            complement(36000..36845)
                     /locus_tag="PKN92_01695"
     CDS             complement(36000..36845)
                     /locus_tag="PKN92_01695"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ47535.1"
                     /translation="MGFISTRLARLHDKKQRRHPDSGILSGAEPRHLGAQDAERAVLF
                     IHGFIATPNSFADLPDYVAARGWHVRAMLLPGHGTSPFDFQKVTGQELEDAVLQEVEQ
                     LKRRYATVVLVGHSMGGALATIAAAQSSPKGLILVAPYYSVTHRWYYMMRPETWARLA
                     SSVTPFICAPGTRQPIRQTTDKGKIILYKWIPLKSVPVAMALAEKARHEDTLRKINMP
                     TLLIHSVNDTVTDPAASKKVYDVIPSPDKTAEWLTRSNHVVFWDYDRDQVKESVSRFL
                     SRWES"
     gene            complement(37006..38826)
                     /gene="ilvB"
                     /locus_tag="PKN92_01700"
     CDS             complement(37006..38826)
                     /gene="ilvB"
                     /locus_tag="PKN92_01700"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNZ47536.1"
                     /translation="MVGLKAKKSIAHGQIMSGAEMTLQVLAEEGVDTIFGYSGGAILP
                     TYDAVFQYNKNNPEKQINLIVPANEQGAGFMAAGYARASGNVGVALVTSGPGATNTVT
                     PVRDCMADSIPIIVICGQVARNAIGTDAFQEAPVYSIMSACAKHVFLVKDPKQLAATV
                     RTAFQIARSGRPGPVVIDLPKDVQNWQGAYDSESVLQLHGYQERTNSVSARRMPEKKM
                     ESFFKMLKQAKKPLLYVGGGAVISGASAVLQCFMKQYQIPAVTTLMGIGALDTTDALC
                     LHMLGMHGAAYANYAVDDCDLLIALGARFDDRVAGIPHKFAANARIAQIDIDAAEIGK
                     VKAVDWSFAGDIKTALSDLLEYGKEFSLDHSEWVAAVQKMKETHAFNFNRESDIVQPQ
                     EVLQELNKITEGNAIIATGVGQHQMFAAQYFDFKKPRTLLTSGSMGTMGFGLPAAIGA
                     QAAFPDALVIDVDGDGSIRMNIGEMETCTTYNLPVKILLLNNHGDGMVVQWQSLYFDN
                     RFSGTDKTLHSKDFVKAAEADGFGFARRVTKRSELEEALKAFVSFDGPAFLEVFVDKN
                     SFVFPMVGPGMAYKDMMTGPYIKSRSAGPTDGTLDTSDSF"
     gene            complement(39007..40938)
                     /locus_tag="PKN92_01705"
     CDS             complement(39007..40938)
                     /locus_tag="PKN92_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNZ47537.1"
                     /translation="MKNLVSQLARGGADLMPADLEADLNEQISQESMSQLMEEAPQNF
                     KEGEVVRGRIVGINDNRVSVDIGYKSEGVVDLGEFAEGESTAIGTAYDFYIETPENDH
                     GAPNLSKIKADRIKNWEHVQAVYDNDGIIEGVITRRVKGGLKVDVGVDAFMPASQLTF
                     RPTGDLDRYIGEKMEVKIVKLTRRRRNVVVSRRRLLEEQREIEKQHLLKRIEVNGLIE
                     GEVKNITDFGAFVDVGGIDGLLHVTDMSWGRVKHPTQMVQVGDKIQVIVLSFDPVSER
                     ISLGLKQRTENPWKTVLDRYPVGAIMRGRVVSMTEYGAFVQLEEGVEGMVHVSEMSWT
                     RRVRYPNEVLSIGDEVDVMVLSVDAEEEKIALGIKQTQPNPWKQLSDRYPIGSAIKGV
                     VRNLTEYGAFVQIEDGIDALLHVSDLSWTKKITNPGEVLQKGQEVEVQVLSIDPEAEK
                     ISVGLKQLQSDPWLSVVEKLPIGAHVEVEIVKLVAFGAFGRLDNSVEGLIHVSELSQE
                     RVNKPEDVLKVGDTVWAKVISINAAERRIGLSMREYQRDQERLEAGEEIPAGESVDVS
                     AALGGSLPQGIYQAGRSLADVAHDLYAAVSRSQNAAAQEQAEAQAASAEDAPAEEPPL
                     SGVPEATVEEAAPEPEQEG"
     gene            41014..41664
                     /locus_tag="PKN92_01710"
     CDS             41014..41664
                     /locus_tag="PKN92_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNZ47538.1"
                     /translation="MVYFQFTAGEDCFFLEAPVWDYSKLGLLEIGILGLGLSIDAFAV
                     AMVASTLLVTVNRRQFFRLSFHFGLFQALMPLIGWLAGQTVEKWIHPWDHWVIFLILL
                     AVGGKMSWEAWSNYRNGTDPLASCSTDPTRGLRLIGLSIATSLDALAVGLTLAVMQVN
                     IFFVVFVIGCITASLTFTGMKLGSRLGLHFGQKVQIFGGFLLAALGMKVLLAHFLS"
     gene            41773..42915
                     /locus_tag="PKN92_01715"
     CDS             41773..42915
                     /locus_tag="PKN92_01715"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNZ47539.1"
                     /translation="MDIQSLMAPFSFAEKEPYAKAMEAAASGKPVAGYLCSYAPLELL
                     HAAGYLPVRVFSRIGDTHHADELLQTFSCSYARSVLDRALKKEWPFLDIVVFSHTCDT
                     MQNVADLWRAHRGDAEVLIVSVPTQTDGALAEKYYGAELARVRCLLEEKTGPIAEARI
                     EEALALFQQQRNAMEQLYALRARYPKVLTGARVMQIALAAQLMDREAHLAALHALLSA
                     VESAEEAASDDSAALPKVFVAGSMCSPPAFIDLIEEAGCHIVGDDLCVSHRAFYSADA
                     EDGSTLEVLTRRSLHRDPCPAFHRTGWHPGHALLARVRAAGADGVIFLLTQFCDPMAF
                     DYVPMMKALEEAGIPAITLSVEQNQEPSEQLRTRVSAFSEMLQGSA"
     gene            42917..43720
                     /locus_tag="PKN92_01720"
     CDS             42917..43720
                     /locus_tag="PKN92_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNZ47540.1"
                     /translation="MNTHTFFAGVDVGSAAVKVVLLDEADQILSQAVLPTSGVLADSF
                     RRALDQAVDSAGLHAQYCRGLIATGYGRDRVEGRIRSVTEITCHARGARFFYPEARSV
                     IDIGGQDSKAIALDEGGRVLRFEMNDKCAAGTGRFLEVMARLLEIELDQFGGRALAAD
                     APVAISSTCTVFAESEVISWLAQNQPVNNIAAGLCRSIASRVFGLASRVRMTGPTIMT
                     GGVARNPGVVHALEELLGEPLLLPGQPQLAGALGAALFAKDSVLSSGGQ"
     gene            complement(43772..44365)
                     /locus_tag="PKN92_01725"
     CDS             complement(43772..44365)
                     /locus_tag="PKN92_01725"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNZ47541.1"
                     /translation="MALEVTKTAPDFTATAVMPDNTFNEKFRLANYLGKYVVLFFYPL
                     DFTFVCPSEIIAFDKKLKEFKERNCEVIGVSVDSHYSHWAWKNTPVDKGGIGNIQYPL
                     VSDLTKQIARDYGVLFDESVALRGLFLIDKEGIVRHALINDLPLGRSVDEAIRVLDAL
                     QFHEENGEVCPANWRKGEEGMKATADGVASYLGKHAK"
     gene            complement(44740..>45153)
                     /locus_tag="PKN92_01730"
     CDS             complement(44740..>45153)
                     /locus_tag="PKN92_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNZ47542.1"
                     /translation="GTVTYDYSGAAPAGQIINQDPAAGLELPAGSTINLVVAAEPSIV
                     PNVVGKTEAEAADLLAAAGLVVGSVSQVYSDTAPAGQVLQQSVAPGSSVAPGTAVNLV
                     LSRGKETTVPAAGAVALVALSAALAGIGLARSRRK"
BASE COUNT         9531 a        12075 c        13169 g        10378 t
ORIGIN      
        1 tcagccaagg cgcggctgtg agtatgtgtg cggtgagcat gcagtcacag ccgcgcatga
       61 gcagaaaagc caggaaaaag tcctgccagt cagggcctac ggttttgtgc cagccgagtc
      121 cgccctcatg attggcgctt tcgccaagac cgcgcagatc aagagcaagg acagcgttgc
      181 cttctttcag aagggcctgt attctttccg ggtttccaaa aagagatgct ttgccttctg
      241 ccgaacagat gagtatccag cttccggtaa aagcctcggg catggcgagc aatgccggga
      301 gtacaatgcc gggctcaggg ttcagagcca gatactggag ggggattccc ttataagtgt
      361 cttctttcag cacaagcgct tcgggcgcag ctgtatcact cgtgcgcaat gcgatcagct
      421 cttttatttt ctcccggata gcagaggggt cagcagaacg gttgaaggcg gtgcgtttct
      481 gtgccagcat attctcacgt tctgaaagca ggtcgaggat ggaacgggcg cctgcttcgt
      541 aaatgacctg acccctgtca gtgcacagga attctttctc cgtaaaaata tcaaaatccg
      601 gctcggtaat aaagcgatcc tgctgcagca gccagcggct catccagcgg accatccctt
      661 cccgctgtgc tttgtgaaat ccgtgttctt cgtcgttttc gataagatca atccgttctg
      721 ccacacccag acgggtatag agtcttttcg cctgccggaa agtggaccag gtgccgttaa
      781 tgtcgaagaa gtctctggtt gccacgcaca taaggacggg gttcggcata tgcagatgca
      841 gatagtcggc atggtcaaac ccgtagctga tctgcccgaa aacattctgc tcggcgtcct
      901 ggggcccgat ggtgttgagc agccggtcga agctggtcag atagcaggaa ggggcagcgc
      961 agacaatacg cggatccagt gccatgagat aactggttag cgtgccgccg cctgagttcc
     1021 cggtgcagcc gattttgtca gcaatgatgt cgtctcggct ctgaagataa tcgatggcgc
     1081 gcatgccgtc ccagacacgg aaggaagcgg tgttcatgcc ggtcagaatc gccttggcac
     1141 ccacaagggt atggtcaata acagagccca gttcgaccga accgtcttct ttgagaaatg
     1201 tgcagcgttc accctgaccg atgggatcgt agcagagcgc cgccgtgccg ttggcagcca
     1261 gaagcatgga agcacgctga taggcatcca taccttttcc ctcggctgca tgcccgcagg
     1321 gaacaatgac gcccggccag ggtccctgtc ctttggggag atatagaata gcggtcacca
     1381 gcaatcctgg ctgactttca aaaatgactt tctcataacg gaagtgctct ttttcgccct
     1441 gtccggtaat acgtgcattc aggggacagc gttccggaaa accgccaaga ctctcagtaa
     1501 aacggctgcg caagttctcc tgccaggcga gaatgtctgc ttccgtcttc agtgcttcat
     1561 aggcttccat gcgcgcgttg agacaccctt ctgcctcatg caggaggtat ttgtacatca
     1621 tggcggcatg cggcgtgcca tccggattgg caggcagcac gctgaaatct gcagctgtgg
     1681 cagttaacgc cagagaaaga caaaaaatca cgctgaaaaa acgcataacc gtctccttta
     1741 agcagttgtt ctatataaaa agtatggcga gacaggcggg caaagtcaaa actgttttta
     1801 ttgcagctgc aaggagaaac ggatgtcctt tgcctgattg tcgcattcga gcataagcgt
     1861 gccgctgcta ctgtcccatg ccgagaggaa ctcggtatca tcgggggcga cggtgattgt
     1921 ttccggtgct ttcggcaggc gtatgcgcat gcgcgcccgg acattttcgg ggcctctggt
     1981 tacaaaggag aaggtgcttc cgttcatgct ttcttcgcgg acacggcagg aggcggctgc
     2041 cacttcagcc gtggcatgat gtgctgccgc ccagtcaaga tcaaagagga ggcagcgctg
     2101 accgggctcc agcgtttttt ctttcacgac aggcaaggcg gcatccagca gatcgataaa
     2161 ggaaccctgc agcgacagcg gctgttcaga aatcgattca tccatcaccg aagcaatcag
     2221 gaacggaccg cgccgcagtt tcatatagtt ccgggtctgc agggtgatcc ctttctgctg
     2281 cagcataagc gccaccgctt gcatgagccg ttcggcgcct tctgtctgtt tagcgaggcg
     2341 ggtcggcgag atgttcatga catagacccg gcccccgtcc accgccacag gcgtgtccgc
     2401 ctgttcagag ctgatgccga gccgtctgaa aaggtcatcc tgcgggaggg catcgttttt
     2461 gccttcatca ttccaccatt cacgcacccg gttgtaggga tcggaaccgt catcgacaaa
     2521 gaggagcgca ccgcctccgc gtacccactg atccagcact tcatgataat cgggtttgag
     2581 cggtttctgt ccgtcatagg tcatgagcag cagatggatg ccgtcgaggg cgcccggttg
     2641 tgtgagggtt tccatctgga cgaagcgggc ggggataccg tgtttgacga ggggcatggc
     2701 aatgccgtag agattgctga gcagggcatc gctgccccgg ggtgcagccc gctgaaacat
     2761 gagagtatcc gaaacgagca cgccgatccc ctgtgttcct gtatcgtatt ggaccttatc
     2821 ctgatccata tggttgaggg cattggtgac ggccatgagt tcggcggcgt agtctgccgg
     2881 gatcccttcg cggtcatccg attttgtgtc aagatccact ttgggatagc tccccttgaa
     2941 gatgcgtgtc ggccagggca tgatttcgta tcggtagttt tccggccaga gcagcgagga
     3001 gatgacggtg cattcataat tctccttgta gttgcgccag ctgtaattcg ggttgtcttc
     3061 gatggggtcg gcgaggaacc agagttttct tcctgtgggc cggaccatgg cgaccatctg
     3121 gctgtattcg aggaagcccg attcgaaagt acgttctttc tgcacgcccc ggtacagatt
     3181 gggcgtacgg gcagtgcctg tccagacctg ggcgatatac ccgtccattt cctcgagatc
     3241 catgaggtgt gacatggggc ttacaatccg ccactgcgca taactgttga ggctgtgggt
     3301 gggcacatgg cattccactt ctctgccctg tgctgccgcc cgttctttga tatggcggaa
     3361 aacttcgcga agcgctttga aatagagttc atatttaagt ttggaggcac ggtattgcgc
     3421 atcggggctt gaatcgggct cctgccacgg ctctccgtag aagcgcagcc attcttcttt
     3481 gaaggcctcg ctccaaccgg cttccgccca gtattcgggt tcttccagaa aaattgcctg
     3541 tgcaccgaga tccacggcag gattcaccac ctgcttgata tagtcgatgt aggcatcggt
     3601 aggcacgtta tagccgacgg tggtgctgtc gccgtgcata tacaattttc ctgatttacg
     3661 ggtctgcact tcatcttttt tgaagccgtc cgccgtgcgg taataatcat catagccgcc
     3721 ccaggcgatg cccgtcatca tgccgatgga atagcccttt tcacgccact gccgaacccg
     3781 gtcggcgaag ctgtcgttaa cgccgtaaac aatcgccata tcggcaccga ggtcaatggc
     3841 aggctgccag tgcccggctt cctgaaagca ggtgcggtcg ggaccttccg cctcagcggc
     3901 cagaccgaac agaagtgcaa agagcagaag agccaaagaa cagcggacaa aggtcatcat
     3961 aaaatccttt ataccagaag aaaattgaat cctgcgggag cgaccatcgc ttgcacagct
     4021 attctagtcg ctgctgtcaa agtatgcaac tatatccggg tcggaagtgt caaggggcgt
     4081 aattttaatt gccagaatgg aattcgaatg gtgctctgag agaacagcca tatcggttct
     4141 atgacctgaa gagaaaagca gatctctctg cagttcatca caaatgatac attccagtct
     4201 tcgcagtgtg ggagggattc ctctttctcg cagtatttta ttaatgggct ttgctttgga
     4261 agagccaaaa gggaccatcc atcctatgcc gtcccacgta cggatataaa gagggtactc
     4321 aacttgccac tcatagaaat aaaagatgcc ttgtgaagcg gcatactcaa tgagatgcat
     4381 aacatgcagc ggttcgggaa atgaagtctg cacagaaaaa tggctgtaaa tgatttcgcc
     4441 gggtgtgggc acggtaggcc actcgtcttc taaagcaggg agattccgaa agatcatgca
     4501 ttccttgccg cgcttgtaaa ggatcagtcc ctccacgaga tcatattctt ttccgggttt
     4561 gctgtccatg agaagacgat ggatccggtt gatatccgcc cggctgaaga caactttgag
     4621 aagccgggca aggacacgca aggcatgacg gacaagcagg ggcggatagc tgcacaattt
     4681 ttcatattcg agataaagaa ggatgttccg ctgatgcccg gcccggccgc attgtttcag
     4741 tacccttccg ccttcttcat cgagatagtg caatgcttcc cgttgaagtg ccgcccgttc
     4801 cgccagctga gctttgcagt ggctgcccag cgagtcgatg accggcagaa ggatgctgcg
     4861 gatacgattt cgtgcatagc gctgatcggg attgctgctg tcttcacgcc agggcacgcc
     4921 tgcactccgg gcacaggcgt caatatcctc tttgcggatg tggagcatgg gcctgaccag
     4981 aaggaacccg ttttttctgc atacaggctc catgccgctt ccgccgaaac tgctcgccag
     5041 acccagcgat cccattaaaa tcgtttccac ctggtcattg agatgatggc ctgtagcgat
     5101 gacttcgcaa ccttcccggt gcgccgtgtc cgtaagatag gcatagcgtt tccgccgtgc
     5161 cagtgcctca aagttctcgc ctgtacggcg ggcatcttct tccaggcttt cccgggcgga
     5221 gcggaaaggg atcccttttt ctacgcaaaa atcttcgacc agctttgcgt ccagcgcact
     5281 ttcccctgaa cgggtgccgt gatcaaagtg ggcggcgata agaggcacgc cgaggcggtt
     5341 caagagatga agcagtacca tggagtcctg tccgcctgac acagcggcga ggatgaggcg
     5401 attttttgat aaagcgcctg cctccgccag caccgcacgt attgatgtca tcagttttga
     5461 ttcgatggag ctaagggtca tggcgtttct ccgctgccgg gtatataaat gaccagttgt
     5521 gtgatacctt cggtttccgg atcggatggc atggcatcag gatagaaaga gaaaggcgct
     5581 tcgtcgaggg gcgccagctc cgcacaaacg gaaaaaggca gccgccatgc gccggatccg
     5641 ttctgataca gctcgcccca gaaatcagga aaagaatgct ccggagaaaa gatctctgtt
     5701 gctgccgggt tgatataaga cgcgccttcc tccctggcag gagagaaaag ggacagctcc
     5761 acccgattcc cctcaatgcg ggcacaggcg atcagcggca gcctgttttc gggaaagccc
     5821 ggcggcgcta ctctgagttc gttgagggga tgcgccgatt tcagaatcgc tgcgagcaac
     5881 gctccgtccc atcggcggaa tttctcttcg agctccggcc tttcgagaag ctgcgccagt
     5941 gcggcagccg cggggagggg attcagtgtt gtgtgcgtct cgacaggatt gcgaatttcg
     6001 agcagcgaaa gggcatgccg ttcccgccct gccgccagca gtgcctgttc ataggcgaga
     6061 ccacggagcg attcagggag agcggaactg gcatctcgca tgtcatcgag aaagcgggta
     6121 atttcctgtg ttcccggcgg cggcaggagc ctgtttctgc cgggcaggag cgactgtgca
     6181 aagcagtgac gggcagtatc cagattgccc agtgccagat gaagtctcgc ttcgcagaaa
     6241 tcggcatatc tttcgccggg acaccggttc tgcacaaagt ccagctgtgc ctgaagtgcg
     6301 gcataatccc ggctgtggag cgcctgttcc gccgtatgtc cgtaatggcg gggatcaccg
     6361 ctgcggatga aagtcagatt aaaaataagc aggagtgcgg caggcagtat cagaagcaca
     6421 gtgccgtgcg gtgcggtctc attccatttc aggcggggtt tggcgcaggc ggcaagaagc
     6481 gcaaagagaa aaacagcgta gccgtcgggc atattgtcgg tcaggagaaa aaataccgtt
     6541 cctgtcaata cagaaagagc aaagagtgca gcaagcgcct gaggcatgtg gctccgtgcg
     6601 acctgcagtt ttgcggcggt gcgtgcggcg agccagagtg ccgtcagggt aagcggtaat
     6661 gcggggaaaa gaaaagcggt tccggaatcg gtcccggaag cgggaaaagt gcctgactga
     6721 tacagaacaa tacccggcaa cagcaccggc agtgtttcca gcagagcaac agaatgcctg
     6781 gaacggtgca tacctgtcat ccgtgcagtc agcagcagaa aaggaagcag cagcacagcg
     6841 atgaggtgaa agggaccgga gcggaaattg gaaggtccca gccagacaaa ggagtttccg
     6901 ggctgaaatg cagtggggaa gagaagcccg tgctgtgccg gcagactgag caggatcacc
     6961 agactgctcg accacagaga gagttgaagc gcccgccaga ccacagcact ccttcgggtg
     7021 ctgtgataaa taagcagaaa gaaaagagaa aaggcaatta tttttccggc tacagcggcg
     7081 gagcctccag ggatcaggag cagtgacagg agacacagca gcgtcgcagg gaagatcagt
     7141 tcgaaaggaa cggtcagcct tttgatgcgc tgccaatctc tgccgaggag gaagaggaga
     7201 aggagcagcg ccggatgatg cagataaaga ggaccggcga cacgtgcgcc gatacccaga
     7261 ggcagtgtga gtgccagcag gcacagacct gtttctgtgg catcctccgg gaaaaaagtg
     7321 gtgtgttctg aaaagacttc gtcgtgcttc tgctgtctct ggtccctttt cacggaagat
     7381 gttcctccgt tacagcgtgg ggcacagccg gtgcttcggc aggggaatgc tctgaagcaa
     7441 gcagatgaaa gagggcggca aggagcataa atccggtgaa aaagagcagt gtcagccgtc
     7501 gctcccgacg cattctttcc agtggcggga ggaaagcggg gcgctcctgc aatgtagcgg
     7561 cctgaggtgt cttcatgtcg tcgctgcttt tttcagacac aggcttctcc cggaccggat
     7621 tgctccggac acttaaccct cctgccggca ggcatcagtt gataaacagt tcgtccggcg
     7681 tcagcgtccg cacttcggcg ataccgccga gagcattgaa atcgaggcgt gctgtatcgc
     7741 ccagcacaga aataagtttt gcttttccgg caatatgttc tttatgaaat gttgcgagga
     7801 tgtcgatact ggtcatctgc tgaatcttct gatacacctt ttctctgggg tccggttcga
     7861 tctgcttgcg ctcccaggag agcgccagtg ccggaatttc ccggaacgaa gtccgggtga
     7921 acgcaaaatc cttgagaagg gtttctcttg cctgtttgaa gcgttccgct gaaaggggca
     7981 tgttgtcgag cagatcgata aaggcgttaa tggcttcagg cgctttatcc gcctgggtct
     8041 gaatcatgcc ctccatggtg ttctgctccg ttcggcgtgc tcccggctga taggcggcat
     8101 aggcggcgta cgcaagggct cgcacttccc gcagctcctg aaaaacaaga ccggacatgc
     8161 cccctgaaaa atactcattg aagagattga cctgtgccat gagctcggga tcatagttta
     8221 cgccgggata ccgtatggta atgtgtgcct gccccatttc gcgctgagtg aagaagatct
     8281 catttttttc gggcgtgcgc atggtgggct gataataagg cagtcgatcc gtcaggggcg
     8341 catcacttct gcacagcgac ccggtaagac gcagtgcctc ttcgggtgtc atcgtgccgg
     8401 aatacaacac atgatatttg taagtaaaaa gacttcgcag cagttcctgc agctcggcgg
     8461 cagtcagtgc tttcagctgg tcggcaggaa gccactggcg ggcagcggat tcttcgccat
     8521 aagccatgta gctggaaagc gccgtcatca gtccgccggg gtctttgcga agatccgcct
     8581 gaatcttcag ggttacggct ttcatagtct ccaggctttc ttccgttact gccggatgct
     8641 caagcatttc ctgcagcagt gccaaggttg ccggcagctg gtcatccagc ccgagaatta
     8701 tcagaaccac atcgttggca gaggcggaga tattgaaaga agaaccccgc ttgaaccact
     8761 caatctgcag ttcgtcgggg ctttttgttt ctgtacctgt ttttgagaaa agatttgtcg
     8821 ctgccgccag acgtttatcc tgccgggtac cgagaggaaa gctaaaggtt aatgaaaaga
     8881 ggtcgttata gggattcggc gtatggtaaa gcgtgatgcc gcgttcgtcc tgcagcttcc
     8941 tgtagtcacg gttttcctga agaaagaccg gtgcgacatc gggctgaggc agtgccagta
     9001 cggcttttgc aagatcagat tgggcagcgg catctatatt aatatccggc agaggcggtt
     9061 tttccaccag tggcgggttg tgtgacccat cttcacggta gcccgccaca tagttctcac
     9121 cgaaatattc attggcgacg cggatcacat cctcccgggt caccgctgcc atttggtcaa
     9181 gaaaacggta agcatcctcc cagggctcgt aagcgagcca ggcatcgcgc atcagaggaa
     9241 cacaggaact gtatttttcc agttttgctt tttctgattt ctgaaaataa ttgttgatgg
     9301 caggaatgag ccagtcatcg aattcgccag ccctgatgat gcgcagttgt tcgaggagca
     9361 gtgcctccac ttcttctttg ctctgccctt ctttgggtgt gccgtagaga tactgcatgc
     9421 cgtagtcatt gaagatcatc gggtaggcgc ctgcactctg cacgcgctgg tttttgttca
     9481 gattgagatt gatcaacccc gcttcggagt tgtcaagcat catatcgagc ataagcagag
     9541 gcagcctgtc gggatgcagg aaaggaaccg tccggaagcc gaggagcaca aattcctctc
     9601 cttcatagga aacggtcacc tgttctcttc cgcgaagcgg atcttcctgc cactgtttct
     9661 gttcgggaat ggggcggggt tcccaggagg aaaaatagcg gtttataatt tccatcgtct
     9721 cgtccggatc gatgtcaccg gctatgaaaa tggccatgtt attgggcacg taccaggtcc
     9781 ggtagaattc ttcgagcttg cggatggacg gtgatttcag atgctccact ttgcccagtg
     9841 tggtctgctg cccgtaagga tgatttttga atgcacaggc ggcgactgct tccgaaatga
     9901 tacggttttt gttgtcctgc gccctgttca tttcttcata caccgtttcc agttccgtct
     9961 ggaacaggcg gaaaacagga tgaaggaaac ggtctgactc gatcattgcc cactgttcca
    10021 gacaattttt cggaagatcg actttatata caacttcctc cacccaggta tgagcattga
    10081 ggttccgggc gccgagagcg gagtaggtgc ggtcaagttc gttaggaacg gaataggcag
    10141 ctgcacgggt agattcttct gtgatctgct gatagatggc tttccgttcc gccggatcgg
    10201 tggcgttgaa atgctgttcg tataattcgg aaacacgctc cagatggggc ttttctttct
    10261 cataatccag ggtgcccagc ttttcagagc ctttgaacag aagatgctcc atataatggg
    10321 cgagaccggt ggcgtcgggc ggatcgtttt tgctgcctgc acgcactgca atctccgcat
    10381 agaaacgggg ttcatcgaca caggcggaaa gaaagacttc cagcccgtta tcaagtcggt
    10441 aatgatgcac atgcctggga tcgtcgggat agggtccccc catagattca aatcctttcc
    10501 ctgcgttttc gttactgtct gctgcggctg cggcaagcac gcatacaaaa agaagacctg
    10561 ttattttcag aacggaagtt ttcatggtct gcataatcct ctcagtgcaa agcgttctat
    10621 tcagcaggcg gcacagctga caggtcagtg gggacagttg gcagcagccc gtccacaaac
    10681 aaagcagcat gccgcccgga aattatatca tgatcaggaa cagattcccg gagaggctta
    10741 tttcattgtt tcttctgctc agttttccag cagcagtgtc acgatttttt taggcgccac
    10801 cgacaggtgc agttgtcctg cctcaagcga aagggtctcc tgccgttttt cttccagtgt
    10861 caggagagcg gcaccggtaa cggaaggcgg cggcgtgaca cttccttcga ttgttctttc
    10921 agtcgggtta aaaagccgta tgacccagcc tgcaccgtct tccgcctgct tgacagcgga
    10981 aagaagcagc gaggcggggg atacggtaag gaatccctgg cgcaggggcg cacggccttt
    11041 gcatacgcct gcctgcaccg gttcaagagg cgccaccaga tcatccgctt cctcgagtac
    11101 ggcaccctcc gcataatttc ccgcatgggg atgaatccgg taagtgaatt catgatgccc
    11161 cggactctgg gaaagaatca tttcaggatg cgtttcccag cggcagctta cggtggcaag
    11221 attgatttca taggcacgga gcagcgtcac cgcaatcgca cggtcttccg tgtcggtaac
    11281 ttcatattcc cggaggcttt cgctgataaa agcaatgccg gcggtgccgt cgctcacgtc
    11341 cgcaaaacgt tgcatgggga aggtgacacc tttggcgcca tgccagacgc tgttttcatc
    11401 gaaggccgtt tcccgctcca ctacatcgaa agcactttct gcatggcagt attttccttt
    11461 gcggtgcgtg ggcagcatga cccggagccg gtggcattgt gcccggttgt cgaaagaagt
    11521 ctgcacttcg aggctccggc tgtttctgcg cagcgtgatg cgggaaacaa tggtcatttc
    11581 ttcttccatt gtgccgcgcg atgcatgatt gcccacaccg tccagccgct gccagggatc
    11641 gccgccgttg tcgtcaatgc ccgaaggaac agacatgcgg tattcaatac ggtaccgtgc
    11701 gaggagcggt ccgtcttctt caagggaaat cacgacagga aaaccccggc tgtcgacagg
    11761 tcggtcatag gcgggccgca cgtgcatcca ggcatggccg ccttccccgt catccacaaa
    11821 gtaatgcaga tcgggatagg aaacgccggt atgtttgtcg aggagcgtaa tcgtgccgtc
    11881 cggccggatt tcggcacgga gatgctcgtt ttccagcgtg ttggcggcgg tgaccagtgt
    11941 gcctgtatcg aagctgccgg agaggtcggc gacaaaagtc gcatagccga ggccgggcag
    12001 ctgtgtcgct tcaaaatgaa tggtgaaccg ctcgctcttc ataaggtttg gtgcgtcaaa
    12061 ggcatgattc atgactgccc agtggggctt gcgcgccgca cactgcacgg gaatggcttt
    12121 gccgctttcc gcatcgcgca aggtaaactg accccgggga cgtggtccct gccacggcag
    12181 atcgacaaca gccgtaacca cttcactccg ctctctcggt gcgggattgt acaccgtcag
    12241 cacgaccgca tccgacggaa gcgcagaggt gtcgatctgt tcctgcatat gggcaagcgc
    12301 ccgggagccg aggctgtcac tgagatcgag cacctgccgg agacgattca tcatatcttc
    12361 ttcaatggca tcaacaccgc tgccgctgat gctgtcgtgg gcatgacagc gcagcatggt
    12421 cttccaggcg tactcgagca tggcggcagg ccagggcgct cccatcagcg ccgcagccat
    12481 caggaaaggc tcgaccgtgc gctgcagttt atattcggcg cgggagctgc gttttttcat
    12541 tctggtgcgg ctcgaaagta cgtcactgta gagatgcaga ataatgggca tgggcttggg
    12601 tacccgccgc tcgcctttca gaacagtgag cttgttccag tcgatttctt cacgaacggc
    12661 acggagcatg ctttcataat tgctgtgcac cacttcatca tcaggcagtg cttcccgggt
    12721 cagccggatc agttccggct ccagcgggtc gggcacactg ctgtcgtggc ccatcatgaa
    12781 gcagagatgc cgggtaacgg acacaccggt ctccgcctct ttcagggcgc gcacagcttg
    12841 ctccagccgt gttttgtcaa tcgtgcgccg gggctgcagg agcagatggt gcgtcagtga
    12901 aagttccggt gatgctctgt ggaaaggcat accgccctct ttccactcgt gttctctttc
    12961 cgcaacggtt ctgccgatga cggcgggacg gtatacgccg tgatagaagt tgtagcgtgc
    13021 gccgctgctc atctgcgacc cgaggatgcg ggtgccgtcg gcgccttcaa aaatccattc
    13081 gttggaaact tcctgatggc tgacgccgtg gtagaagagc atggtgtcaa tgccgaaccc
    13141 ggaatagatc tgaggcatct gtgaattctg cccgtaggaa aaaggggtgt gccccacttt
    13201 catgacatga ccgaacccgg aagcaacacg atgacccatg agcaggttgc ggacgagtga
    13261 ttcgccgttg acttcaaagc cttcagggca ggtgtaccag gggccgacca gaagccgctc
    13321 cgcctcaatc agcgctgcga gacgttccct gttttccggt cggaattcca gatagtcttc
    13381 cagcggtacg gtttgcgagt cgagcacata ggaagtatag gaatcgtcgc cttccatcac
    13441 ttccagcagc aggtcgaaaa actcgaccag catcattcgg gtttcctgaa aattattcag
    13501 ccattcccga tcccagtgcg tgttggaaat cacatggatt acatattttt tgctcatgaa
    13561 cagaaccctt gtttaaaaga aagaagaacc gcagtccgga gctgtccgga cagcgtgacg
    13621 gcgtgcagcg caggctcgaa atataaacta aaagaggcgg cagtccttag taccggtacg
    13681 tgtccggttt gtagggaccg tccacaggaa cgcccagata ggacgcctgg cgcgctgtca
    13741 gtacttccag cttcacgccg agcttgccca gatgcaggcg tgccacttcc tcgtccagtt
    13801 tctttggcag cgtatacact ttgcccgctt cgtatttgtc aggctcggtg aaaaaggcaa
    13861 tctgcgccag cgtctggttg gtgaaggaat tggacatgac aaaggacggg tgaccggtgg
    13921 cgcagccgag attcacgagc ctgcctcggg caagcaggaa aatcgcacgc ccgtcgggga
    13981 aaatgaattt gtccacctgc ggcttgatat tctcttcgga gatggaagga ttcgattcga
    14041 gccaggatac ttcaatttcc gaatcaaagt gaccgatatt gcagataatc gcctgatcct
    14101 tcatctgctc catatgtcgc ccggcgatca catcgcagca gcccgtggcg gtaacaaaga
    14161 tgtcgcccag cggtgccgct tcgtccatgg taagcacctg atagccttcc atggcggcct
    14221 gcagcgcaca gatcggatcg atttccgtaa tgatcacccg ggcaccgagt ccgcgcatgg
    14281 cgtcagcgca gcctttgccc acgtcgccgt agcccgccac gacaaccact ttgcccgcca
    14341 ccatgacatc ggtggcacgt ttgatgccgt cggcgaggga ttcacggcag ccgtagagat
    14401 tgtcaaattt ggatttggtt acactgtcgt tcacgttcat ggcgcgggtg agcagtgttc
    14461 cttcccgcat catgtgatac agacggtgca cacctgtcgt cgtctcctcg gaaacgccga
    14521 tccagttttt cgcgatgcgg cgccagtagc ccggatccat ttcgtgcact ttgtagagca
    14581 gcgaatcgac cacttcgatt tctttgttgt cccggcagac aggcggcagc gcaccggttt
    14641 cctcgaaact tttttcaaag tcgcagcccc ggtgaatcag cagcgtggca tcgccgccgt
    14701 catccacaat catgttcagc gtcttgccgt caggataatt catcgcctgc caggtgcacc
    14761 accagtactc ttccagcgtt tcccttttcc aggcgaaaac aggtacgccc gttgccgcaa
    14821 tggcggcggc ggcatgatcc tgcgtcgaaa aaatattgca gcttgcccag cgcacttcgg
    14881 cacccagcgc cacaagcgtt tcaatgagca ctgccgtctg aatcgtcatg tgcagtgagc
    14941 ccatgatacg tgcgcccttg agcggctgcg cctccgcata ctggcggcga accgccatga
    15001 gaccgggcat ttcctgttcc gcaatttcta tttctttgcg tccccattcg gcaaggccca
    15061 tatcggcaac tttaaaggga agcgtgtcgg tcagttcggg aatggcggca agcactgaag
    15121 acataggata aaaacctcct gcaggtaaag ggttggttga gagatttcag ttggatagaa
    15181 gcccttgtcg ggcagaaaaa aaacagacgg gaagccagtt tatgcttcag acgacaaaag
    15241 atgcgctgtg aagacggcgc cgaactcagc gaatatcggc atccagccat ttaattacag
    15301 ccataatgcc ccgggcgcgc ttcagctgcg tcgctttcag accgtgttcc cgcagcttgc
    15361 cttttgcttc gcgccgatcc tttgccacga cgatcccgcc gataaaggat ttctcttcgt
    15421 ctccttcggc gcgaatcgca tgatactcat acacaggcat ggatatactc cttcatgctc
    15481 aaaaaagaag catcagtaca catatataat accatttacc gggaaagtat tcacaatccg
    15541 gggcagatgt gaaaaaatgc ggcatcagta caccccggga gcgcgtacgg gcttttccag
    15601 aatattaata atgcagatgt atatggcgtc gaagacgctg tctctttttt atcccgaatt
    15661 cctgcgtttc ataagagaac ccgcaccctt tctccggggc tgtgtttctt tctttacgcc
    15721 gtgataagat agagggcgtt ttgaaatgat cttttttatg ttaagaccga cgggatctct
    15781 gaatgcccca gaatacagct attgtcattc cctgttacaa tgcgggaaac cgtcttgaat
    15841 cggtactgaa cgggctgact tcctttgagg gcccggtgat tgttgtggat gatggttcca
    15901 cggacggggg gacggactgc ctgtcggaga ggactgtgca ggcacttcgt tttccggaga
    15961 accgcggcaa gggtcatgcg cttcttgcag ggattcgcga ggcgcttcag catgaagctg
    16021 tgcagcgcat tgttctgatg gatgcggacg gtcagcacga tccgggcgaa gtggatgctc
    16081 tgctttcagc gagcaggtcg acgggggcgg cattggtggt gggtgcccgt cagatcgacc
    16141 gcaggctgat gccgctgcgc agccggttcg gcaatgtact gaccgcctgg atcagcgccc
    16201 atgttttccg ctgcccgctg tccgatacgc aatgcggttt cagagtgctg tcccgtgcct
    16261 ttgcggagtc atttcttgcg cagagccgtg gcggtcgcta cgagacggaa atggagatga
    16321 tcctctttgc cctgcgtgag ggctgtgttc tcgcatcggc gccggtcgcc accgtgtacg
    16381 agcccggtaa ccggagcagc catttccgga agatccgcga ttctttccgc atttaccgtg
    16441 tgctgcttcg ggcgtggcgc cgctgattct tctggttcag ggtgcggcag ggggataata
    16501 tttttccgcc cattccggat tgatgcgtgc ctgcacttcg aaagcatcga gcagcaccca
    16561 cgccgccatg ttttcgacga caggcacggc acggacgacg atgcagggat catggcgccc
    16621 tttgacttcc acatcgcagg gttcaccgga gatgcgtatc gaacgctggc gtgatgcaat
    16681 ggaagcggtc ggcttgacgg cgatgcgcat aatcaggggc gcaccgctgg agatgccgcc
    16741 cagaataccg ccatgatggt tgctgagaaa tccctgctcg cccatgggat cgttcgcctg
    16801 cgaaccgcgc agccgggtga tggcgaaacc atcgcccact tccacgcctt tgatggcacc
    16861 gatggtcatg atggcactgc agaggcgcgc atccagtttc ccgaagacgg gatcgccgag
    16921 gccgggcggc aggttatgca cttcaagctg gatgataccg cccagggagt ctttttcgct
    16981 ccgcgctttc agaattgctt cttccatgag cggtgcggca tcgggatcgg cgcagcggac
    17041 ggggttggtt tcaatgatcg aaaggtcgca tttggaagcc tggatgccgc cgatttcgat
    17101 ggaatgggca acgatgcgga tgttcatgga ttcgaggacg gatcgggcaa aggcgcctgc
    17161 cgcaacacgg cacgctgttt cacgccctga ttggcggccg ccgccgcgat gatcacggac
    17221 tccgtatttt ttatagaagg tgaaatcgcc gtgtccgggc cggaacagat ctttgaggtt
    17281 atcatagctt tttgagcgtt ggtcttcgtt gcggataacc attgccagcg gcgccccggt
    17341 ggtctgccct tcaaaaacgc cggagagtat ttcgacggta tcggcttcgc ggcgttgcgt
    17401 tacgactttg ctctgcccgg gtctgcgccg gtcgagttct ttctggatgt cttcctcgtc
    17461 aaaggggatg ccggggcgca cgccgtcgag caccacgccg atggctctgc catggctttc
    17521 gccgaaggtt gtggcacgaa tcacttcgcc ataggtgttg gcgagatggc ggcgcagcgc
    17581 cagttcggat tcgatatcgt tggcgagggc ggcggctttt ttatccggat cggtatcggt
    17641 ggtatcgaaa aggatgtctg caaagggggt cagtacttca cggcggaaac ggcattgttc
    17701 ggcgaatcgt tcgaagccgt catctccggc aaaggcgggc ggtatgccgc gacgcgttgc
    17761 ccgttcccag aggatttccg gctcggcgca gaaaaggaca aggatattac ccggtcgcag
    17821 cagtcttcgt gcatcggggg acatcatcat gccgccgccg gtgatgatga catgataaac
    17881 atgctttgcc acttccgccg ctgcctgcaa ttccagttcc ctgaagcgcg cttcgccttc
    17941 tgcagtaaag atttcacggc agctgcggtt ctgtccgtcc agtgcttcaa tggttctgtc
    18001 cgtatccacc caggggagtc ccagctgctt tgcgagtttt gttcccagcg tggttttgcc
    18061 ggcgcctttg gcgcccataa tgacgatgtt catgcgcaat cctttgtcac gacgcttcga
    18121 tgccggatcc agagagactg cctccgagag cctgcaggtg ccggaaaaat tcggggtagg
    18181 ttactgtaac agcttctgtg ccccggatgg tcaccggtcc cgttgccgct gtggcggctg
    18241 ttgccagtgc catgataata cggtggtcgc cgtgtccgtc caccactcct tcatgcaaca
    18301 cgccgccctg aataatcatt ccgtcttcca gttcctcgat ggcggcgccg agtttttcca
    18361 gctccatccg catgacccga atacggtctg tttctttaag tcgtgcctgc ggcacattga
    18421 ccagacgtgt ggtgccttcc gccagacagg cgagggctgc catcatgggc agtgcatcgg
    18481 gcgtggcgtt gagatcgaat tcacagcccc gcagcttttt ggccgctacg gtcacttcgt
    18541 ctttttcaca gcgaagtact gcacccattt ctttgacgaa actgacaacc gccttgtcgc
    18601 cctggctgtc gttcatatcc agtccggcgc aggcgacgcg gttgccgggg agcgcaccgg
    18661 cagcgagaaa gaaggtggcg gaactgaaat caccgggaat gggccggttg acaggcaggt
    18721 actgctgccc tccgggtata tgatattcgc tcaggtcatc gctgtactgc acggcaatcc
    18781 cctgattttt cagccagtca agggtcatga caacataagg cttttcgttg agcaggggca
    18841 catgcagccg tgtatcgcct tccgccagcg gtgcattgag cagcagggag ctgacatact
    18901 ggctggtgac ggcttccatt tccgtttcgc cgccgcggag catgccgccg acgacccagg
    18961 gcgggcatcc gttgccgcgg gtggactgca cctgtgcgcc cagagcattc aacgcctgcg
    19021 ccagcgggcc gcccggccgc cttcgcacct gatcatcgcc ggtgagtacg gtcagtcctt
    19081 tcggaatcag cgctgcgctg ccgagcgcca tgcgcatggt ggtgccggag ttgcctgtgt
    19141 ctacgatatt gtcgggcgcc tgcggccttc cgtttgtgcc gacgatgcgc cacatctccg
    19201 gttcttcggt gatctgtgcg ccgaaggcat tgtagacgtt gcgggcggat cgggcatcct
    19261 ccgattccag aggattaagg atgcagcttt cccccggtgc cagtgcggca atcactacag
    19321 cccggatggt atgggacttc gaggcaggga tttgtatcct gccccgaagt gttgaaggtc
    19381 ggattgtaac ttccacgcct gtttctttct gcagcgcagg gagttgcgga cggagtggct
    19441 gtgtcagcct cagatcacgc cgagttcctg catgtctttt tccagctgtt ttttctctgc
    19501 agcggtgagg tcacgctgcg gcggacgcac ataccccgca tcgaagccgc ggagccgtag
    19561 cccttctttg aaagaggcga ggttctgacc gtacttgaaa atgcgggctg cccgctcaag
    19621 ccggatctgc tcctgccagg cttttttcag gtttttcttc tggatattct cgtagaccgc
    19681 cgcatagatt tcgcagacca cattggagag cccggatacc agagcggggc agccggcgag
    19741 aatggcctgg aaaccgtaat catcagtgcc gcagataaca tgaaagccgt cgggcgcatt
    19801 gccgatcatg cgggtgagtg cggtcataac accggaactg tctttgatgc ctacaatatt
    19861 ttcttccgat tccgccagtt tcagcacaag ttcagggctg agtacgttcc gggcgcaggc
    19921 gggaatgtta tagagcagaa tggggaagcc gtccacggca cgggcaagat ttctgtaata
    19981 ctgaaagagt ccggcatcgt cataggcgta atagcctggt gcgacaatgg aggccgctgt
    20041 ggcgcccgca tcacgggcat ggcgtgtcag ttccagcgtg gtggcatagt ccagacagcc
    20101 ggtatgcgca atgacgggtg tctttttcgg cgccgtacgg agcacttctt ccagtgtttc
    20161 cttacgttct tcggcagaca tgagcatgcc ttcgctcgtg gtgccgcagg gaaacagccc
    20221 atgagctccc tgactgagca ggcggtttgc cagcggaccg atcttgtcgt agtccacatt
    20281 ttcgccgccc tttgtaaaag gcgtaaccat ggcagccatg atgcctctga ttttgatttt
    20341 cattgtcata actacagtct ccctgtatgt ttttgcccgg actttctgcc cgggcactga
    20401 ttaaaccgga ttattatgtg cagcaaacgc cagccgtcac ggctccgcct gcagacgttc
    20461 ctgtgttgcg ccgaactgaa tctggcagat tgccagcgcc agcgcatcgg cggcatcgtc
    20521 gggccgtggc acttcgggca gattcagcag ccgccgcacc atttcctgca gctgcttttt
    20581 gtctgctctg ccgtagccgg tcacggcgat cttcatttcc gtgggactga actcgcctat
    20641 gggacagcct gcctgtgcgg cggcaaggag cagcacaccg cgtgcctgcg ccacctgaat
    20701 gcctgtggtc acattctgct tgaagaacag gcgttcaatg gcaacggcat cgggctgaat
    20761 gcgggctatg agtgcctccg cttcctgaaa cagagtctgc agccgttgtg cgctgtgctg
    20821 ttttgccgga gtccggatag tgccgtgttc acggtggtgc aggcggttgc cctgcctgct
    20881 gaccacaccg aaaccggtaa gggcgctgcc cggatcaaag ccgatcacca gcatttattc
    20941 gtccatgact tcagccatag cctcgtcggt aatgttcaga ttggaaaaaa cgtcctgcac
    21001 gtcatcatga tcttcgagtt cttcgatcat ccgcaggcat gagagcagct gtttgcctgt
    21061 cagctccatg gtggtcttgg gcatcatggt cagtttgact tcttctgctt tggttcctgc
    21121 cgcttcgagc gctccggcga caacatgcag ctgcgtggga gcggtccgaa tttcataggc
    21181 atccccgtcg ccgctgacat cttcggcacc cgcctcaatc gccttatcga acatttcttc
    21241 ttcggaaatg ccttccttgg gaatggtcag gagaccctgg ggctcaaagt tccaggcaac
    21301 ggctccggct tccgccatat tgccgcccaa cttgctgacc agatggcgaa tctctgacac
    21361 ggtacggttt ttgttgtctg taagcacgtc gatgatcagt gccacgccgc ccggtgcata
    21421 ggcttcatac cggacttcat cataggtgac gccgggaagt tcgccggtgc cttttttgat
    21481 ggcgcgatcc acattatcgc cgggcatgtt ctgtgtacgg gcagccagaa gcacggaacg
    21541 taatcgaggg ttagcagccg gatcgccacc gcccagacgg gcggcaacgg tgatttcttt
    21601 cgccagcttg gagaagattt tcccgcgctt ggcatctgct gcaccttttt tacgtttgat
    21661 tgtactccac ttggaatgac cggacatttg gaattctcct ccgtacttgt aggtactggc
    21721 gtcacctcag gtgacaggta aaagtgtcat agaagattca tagtatacca ttcccggcat
    21781 atccccgcaa agaaatagga cggaagcgtt tcggaaaaga ggatcaaaaa ggaagattaa
    21841 actgttgaca ggacaaataa atcttgatat tacaggtaat atttggtagt ctattaaaaa
    21901 gtgtctttgg tggcttttac ggcggctgca gcgccgcagt cccggaaaaa taacagcggc
    21961 cggatgatct cccatgtatg aatcctttta cgggcttcgt gagcgtcctt tcaatctgac
    22021 gcccgatccc aaatttctct atctgagcga aaagcacaaa gaggctttcg ctcatttgct
    22081 ctacggcatc aagaaccgta gcggattcgt gatgatcacc ggcgaaatcg gaacggggaa
    22141 gactacgctg tgccgcaacc tgctcaacca gcttgattcc aatacggaag tcgcctttat
    22201 tttcaatcct ttcctgagtg ccatcgagct gatgaaaaag atcaatcagg aattcggaat
    22261 tgaatcggat gcggatacag tgctggagct gaccgaagag ctgaatgtgc atctgctcca
    22321 ttccagtgcc atgggaaaag actgcgtgct cgtcattgat gaagcacaaa acctttcgcc
    22381 tcaggtgctc gaacagatcc gccttctctc gaatcttgaa acggatacgg aaaaactcct
    22441 tcagatcatt ctgatcggtc agccggagct ggcagaaaaa ctggcactgc gtgaactgcg
    22501 ccagctgaat caacggatta cggcacgcta tcatctgaaa tcgctgagcg ccagcgaaac
    22561 gctccagtat atcgcctaca gaattcatgt tgcaggcggg cgcaaacgca tcaattttac
    22621 caaggcggcg gtgacccagg tctacaaact tgctggtggc gtgccgcgca tgattaacgc
    22681 cttatgcgac cggacgctgc tcatcggcta cacgaaagag cagcatgtta tttccgcaaa
    22741 actggtgaag caggcggaga aagaaatccg gggtgaaaaa attacggcac ccaaagaacc
    22801 ccgcacccgg tggaggcggc tgatcccttc gcctgccctg ctgctgattc tggctgttgc
    22861 gatcgtgctg atacattatc ttgtgtcgcc cctcgatcgt tttgcgcagg agctcaacca
    22921 cttcaaccgg atcttaacgg gtagcaccga agcggtgccg ggcacgcccg aaagcggggc
    22981 agcgcggaca gcggcagaag cggcgagcga tgcgacttcc ccggcggtgc ctgtgcagcc
    23041 agcgacggaa gagccgctgc gccaggtgct gaaccggctg aatgcggcag gagccggcaa
    23101 aaatgcacag cagctggcgc ctgtgcttgc ggagctggat gcggaaaaag cccttcaggg
    23161 cgggctggaa acgctcctgt ctctgtggaa ccggccgtcg ccgtcagagt atccggcaaa
    23221 aggtgatgcg gacacactgg ttcgcttttt ggaagcccag ggtcttgcca gcgagcatat
    23281 ccgcccgtcg gcggagcatc tggccgccat caatctgccc ggccttgtac ggcttcatgc
    23341 cggagagatc acctgctggc tggggctggt gcggatggat caggaaacgg tgacactcct
    23401 tccggcagtg aataagcgta ttatcgttcc tttaaaagag ttttccctct attacgccaa
    23461 agaggcagtg ttctcctgga atgatgccgg tttctcggga cccgtcctgg tgctgggtca
    23521 gaagggccct cagattgaag cattgaagga aaaactgcgt cgggcgggcc tgctggatgc
    23581 aggcaataca ggcgacactt tcgataaagc cactgaaaaa gcggtgatgc gcattcagga
    23641 gctggcgggc ttgaaggcgg acggaaaagc ggggcgtcag gtgcggcttg ctctggcaag
    23701 tttcagcgct gatgtgccga ccctgcagga acgcccggcg gcgggaaatg cggccgaaag
    23761 cgaaggcggt gctccggcag atgcggcagc cattacggcg gcggaaacgg tcagcaaagt
    23821 cccggcacct gcaaaacagg atcccttgaa aaaagccgtc gtcgctgaaa aaacggcacc
    23881 tgtccggaag ccttccgggg aaactcctgc acagaaaacg gcggcgcctg cagccgaaaa
    23941 agaaattatt tttaccgctg ctgaaccttc tgctgcatct gaaagtaaca cggactcatc
    24001 cctgagcgaa gagcgtgatg ctgcagcgcc gggccaggaa agcagtctgc tgcgcctatt
    24061 gggtgcgcct gcggacggcg tgcgggaaga ggaggaaaaa gtctcggccc cttctgtgct
    24121 gtcagtcggc ggagaaaaac tgatagagga acgcgaattg cctgaaccgg gtgttccttt
    24181 tatcgcaccc ggatatgccc cggagggttt gccctcctct gccacgatgc ctgtaacggg
    24241 cagttcgcca ctggtgcccg ccgcccggga aagtgcatat caatgatcag ctgcagcgac
    24301 ttcaggaatg tgaaaggcac cagtgcatga gttccattct ggatgccctg aataaactgg
    24361 agcaggaaaa agcggcgaag cagtcgcagc ccggcgatta tgagccgacc ctctcccctg
    24421 aagaggcggc atctgtcctc cttggcgaat cctctgtgcc tgaacgtgaa aagcggatgc
    24481 cggtctggtt gcctgttgtg ctggcgctgt gcggcggttt tgtactggcg ttggttcctg
    24541 tagggtattt tcttgccgtc aaaggcgggg caacaccgcc cgaacaggtc gctggcgcac
    24601 cggcagctga tacgcacagc tatcagatgc ctgtgacggc gcctccgcct gtggctgcac
    24661 ctgtaccgga agagaaaaag gctgcttcga aagcagaacc tgccggagaa acgccgccga
    24721 cacctccgcc tgcaccccca aaatctgccc cggcggcagt gccggaaaaa aaagaaacga
    24781 cccggccccc gctgccggaa tccatcccgc tgcccgatcg ctttccacag gaaacatcgg
    24841 cgcctccgcc tgtggtcaaa gtggcggatt tgccgccgcc cgttcctgag cctgcacctg
    24901 tacctgagcc tgttgccccg gtgccgcctc ctgcgcctgc acccgtctcg gagcctgcac
    24961 cagtgccagt gcctgagcct gttgccccgg tgccgcctcc tgcgtccgtg cctgcgcctg
    25021 aacctgttgc tccagcgcca cctccggcac ctgtacccgt ccaggagccc gtggttgttc
    25081 ccgttcctgc gcctgttcct gcgcctgctg agcgaagtgt gattgcccag acgccgtcag
    25141 aatacccctc cgcctctgcc gggacagtgc cgcgtgttcc gtcggcgcgt tcgtccggcg
    25201 gtgcggtaag tgtggacaat ctcccgcgtc tgtcttccca ggagcgtgcg cgcttcggtc
    25261 tggacgggct gcgcgtgaat gtgctgcgtc ctgctgataa agatcagcct gatgcgcttg
    25321 caattatcaa tctgaagaaa gtatatgtcg gtgaaaccat tccgggtaca cgggcgcgcc
    25381 ttatcggtgt tgcctcctcc ggcatcggca tcgagataga agtggaaggc agcaacaaac
    25441 aattccggat cccccgctag gattcgcaat tacaaggaga attttcgatg agatacataa
    25501 cgacaggcgc agttctgttg tcgctgattt gtcttgccgg ctgcggcggc agtggaagcg
    25561 gcagttccgg ttccggcggg gcgaagagcg gtgctctgga aattgctgtg atccccaaag
    25621 gtttgtctca tcagttctgg ctgggtgtga aggcgggagc ggaagcggct gcagccgagt
    25681 gcggtgcctc tgtggtctgg cagggtcctc ccaaggaaac cgagatcgcc cagcaaatca
    25741 atattgtgca ggacatgatt acacggggcg tgagtgccat tgtcatggcg gcgtgcgatg
    25801 aaaatgcgct gggtgccgtc atcgcacagg cggacagggc gggcattcct gtcgtcatgg
    25861 ttgattcggg tgtgaagtcg gatattccga aaagtctcgt tgccacggat aatctcgaag
    25921 gcgccaaaat ggcggcacgc gtgctggcgg atctcatggg aggacagggc gaactggggc
    25981 tgcttccctt catttccggc gctgcaacgt ctgaaatccg tgagcgcggt ttcaaggaag
    26041 gggtgactga attcccgggc atgaaaattg ttgccacgaa ctatacccag agtgatgtca
    26101 gtacaggaat gaatgtgacc tccgatatgc tgacggcaca tcccgatctg ggcggtattt
    26161 tcgcaaccaa cgaacccggc gccatcggtg cagctcaggc actccgggcg gcaggaaaag
    26221 ccggaaaggt caaactggtc ggctttgatg cgaccgaaga agagatcaat gcgctgaaag
    26281 aaggagtgat tcaggcactg attgtgcagg atcctttcaa gatgggctat gaaggcgtga
    26341 aagcggctgt ggcatcgctc aaaggggaag aggtgccgcc gatcattgac acgggagtca
    26401 cggtggtgac gctggaaaat ctggaaagtc cggaagtgca gaagctgctg aatacgggca
    26461 aatagccctt tgaaatttct ttgacgcaat gcctgaaaag gagaacggga acgtcgctcc
    26521 ttttcaggtt tttttattgt gcaggttgaa gcgctgccgg tcaagggtct acactgaggc
    26581 tgtgcaatgc tgcgcttttt tctgaaaaca tgaggtgcca tggatcagcc tttttccaaa
    26641 gatatgctgc cgccggagag cacggagatt attctggaaa gtatttccga cggagtattc
    26701 acggtagatc atggatggcg tattacctcc ttcaaccgcg ccgccgaaga gatcaccggt
    26761 attccccggg aagaagcact gggcaggcgc tgctgcgaag tttttcgcgc cagcacctgc
    26821 gaagaggaat gcgccctgcg tcacacgctg gattcggggg aacccatcat taacaaatct
    26881 gtctatatcg tcgatgcggc aggagagcgc atccccatca gtctttcaac tgcactgctg
    26941 aaggatgaaa ggggaaatgt tgtgggaggt gtggaaacct ttcgtgatct gagtcttgtg
    27001 gaagagctgc ggcgtgaaat tgaacatcgc tatgacttgg gcgacatggt cagcagaagt
    27061 gccgccatgg aaaaactctt tcagatgctg ccgcaggtgg cagccagcga ggtgcctgtt
    27121 gtcatagaag gagaaacagg taccggcaag gagctgctgg cacgggcaat tcatgatctg
    27181 agcggccggc acaaaggacc ttttcttggc atcaactgcg gcgcattgcc tgatacgctg
    27241 ctggagtccg agctcttcgg gtatcaggca ggtgctttta ccggagcgga caaagacaag
    27301 cccggccgct ttgctctggc ggaaaaaggg agcctttttc tggatgaaat aggggatatc
    27361 agccctgcca tgcaggttcg gctgctgcgg gtgctccagg aaaagcagta tgagccgctg
    27421 ggcgcaaccc gcgcatttca cacagatgtg cgcatcattg ccgccaccaa caaatcgctc
    27481 ggcgcactgg ttcaggaagg acttttcagg caggatcttt attacagaat caatgtgatc
    27541 aggctggaac tgccgccgct ccgggagcgc cgggaagata ttcccctgct cgtggaccgg
    27601 tttatccgcc gtaacaatca tctccggcag aataaaacgg tagaaggggt cactgcggag
    27661 gcaatgcgcc tgctgatgtc ctacgactat ccgggtaata tccgtgaact ggagaacagc
    27721 attgaacatg ccttcgtgct gtgtgacagg ccgctcattg atgtcggatg tctgcctgaa
    27781 gcgatgcagg ctttttaccg ccgctccggc gctgggactg ctccgtcgct gaacaatgcg
    27841 gttcaggaag cggaggcccg tgccattctc gatgccctga aagcgtgcga tggaaacagg
    27901 cagaaagcgg ctgatctgct gggcatgcat aagagcacgc tgttccgcaa ggttcgtgct
    27961 ctgggcctga cgctccctcc tgcgacgcgt cgcaaataag tcgcactatt gcgactgttg
    28021 cgtctctaat agtcgcatta ttgcgactta tctcgccgaa aatgcgatcc cgctctcctg
    28081 taattgcttt aatatcagta tgttaaaaat attttgatca ttggcacggt gtatgctcta
    28141 agaaggcata ctaggagctg ctgatgaaga ttgcactgac agtaagggaa caacgcattt
    28201 ctccggtgtt ggatacagcc cggacgttgc ttgtcgttga gctgcagcaa ggggttgtgg
    28261 tgtcccgtca ggaaaaagaa attgccggag gcaccgtaca gaatctgctt tcacagcttg
    28321 aagcactggg cgtaaggcag ctggtgtgcg gcgctgtatc ccggcgtttt gccgactggc
    28381 tttctgccgc cggcattcag ctgatcccgt ttatctccgg tgatgttgaa gaaatactgg
    28441 cagcagtgct cgcaggcact gtctgcagcg gcgcttttgc catgccgggc tgcggttccg
    28501 gcgggcgccg gtgcggcgcc ggaaaaggac agggatacgg cagaggcaag tccgggtttt
    28561 ccggagggca ttccgtatgt cctgagtgcg gctgccgtct tcccaactgc cggaggcatc
    28621 cggaatcagc cgtatgtccc ggctgcaacc gccccttcgg gcatgagtga ggtagcatga
    28681 tgacagtgag cacacaagag gcaacagaca aacagaaaac gccctggcac ggcagagggc
    28741 ttaccagttt tctgatcagc ctggtttttc tgacactggc ggtgtccggt ctcgtgcttt
    28801 accttgctcc gtcctgtcgc attgcccggg aaacaggctg gcgtgttttg ctgctgtcga
    28861 aagatcagtg gaatgccctg cacatcatta tggcctgcac ttttgttgtt gccgggttga
    28921 ttcatgtgta cttcaactgg cgcattctgt tgcgctatct gtttttaaaa cagcggcttc
    28981 atttgaaaaa agaagcggtc acggcactcc ttctttttct gctgctgtcg gcaggtgcca
    29041 tttttgaagt gccgccgttc agcttcctta cagcattgac cgaagaggag aaagccggaa
    29101 gctgtgccgg tgtgctctcc gcagaggcag cgcatcctgc atcaggtcat ggtacaggtg
    29161 tcggtcgcat gaccgtgcag gaattttgtg acgatgccgg gcttcttctg gaagacacac
    29221 tgagtcgcct gcaggaagcg ggtttcacgg cggatcccgg atcgacgttc cgggcgctgt
    29281 cgcagcagaa gcagatgtct ccgatggatg tgaaagaatg gctgctgaac agaggcagcc
    29341 tggacaaaga agaaaggaac tagattatgc ctggaagaga tggaacagga ccttgcggaa
    29401 aaggcgccct gacggggcgt ggtgcaggcg ggtgcctgcg tgacggtcgt ggtatggctg
    29461 cgcgccgtgg tgcgggttca ggccgcggta tgcgcggcgg tatgggtttc ggtcgtggtt
    29521 tcggtcgtgg tttcggttta agttccgaaa cagagtcaca gtccctgcgc gaacaggccg
    29581 cccggcttca gggcgcactg gaagaagttc aggaccgtct ggcttctctt gaaaagtcat
    29641 ctgaccgctg acgcagcgga agcagcagtg ccctgcacgg ctgccggaaa agaaatgatt
    29701 atgaaaattg cattttcgac atcaggaaaa gatctgaatg cgcttgtatg ttcccgtttc
    29761 gggcgtgccg atggctttct aatttatgac accgacacag acgagtacac ggtggtggag
    29821 aatcagcaga atgtcaacgc gcctcaggga gcaggcatac aggcagcgca ggccgtagtg
    29881 aaagcgggag caagtgctct cgttacaggg cattgcggcc ccaaagcctt tactgtattt
    29941 gctgcatcat ccatacgggt ttatcattcg gatgaagtac cgatttcgga ggcgctgcag
    30001 gcgtggcgcc gtgatgccct cgcggaaatg catgctgccg atgtggatgg ccattgggtt
    30061 tgagcaggaa actgctcctg ctcagaccct ttgtctgacc cccgacaggc atgttaatta
    30121 tagagtttct cttttctcct tactgacgca ctgtccgttc aatcggcaat tccggaaccg
    30181 gagtcgctcc cgacggcccc ggcaggtttc ggaattgccg ggacagtgcc ctgaaaaacg
    30241 gaaacttcca ttcaggcaga accatcagga tatttttctt tcatgcacat agccatcgca
    30301 tcaggaaaag gcggaacagg caaaacgacc gttgccgcca atctggctgc ccttacggag
    30361 ggtgacgaaa ctgtttatgt ggattgcgat gtggaggcgc caaacggtca tctttttctg
    30421 aagccggagc tttcattttc tgaaactgcg ggaattcctg tgccgcaggt ggatccggcg
    30481 ttatgtaccg gctgcggcaa atgcgtggaa gtctgtcgtt tcaatgcgct tgcctgtgtg
    30541 gcgggaaagc tgattgtttt tgcggagcta tgtcacggtt gcggcggctg tgtgcctgcc
    30601 tgccctgaaa aagccttgac tgaatcttcc catgcgatcg gtacggtcag ccgtggaatg
    30661 gcgggcgatt tacattttgt gcaggggacg ctgcgcgtgg gcgctgctat gtcgccgcct
    30721 ttgatccgag cggtaaaagc gaaagcgccc gatgcggcag tgattattta tgatgcgccg
    30781 ccggggacct cctgtcctgt gatcacgacc ctgaagggca tggattacgt tgtgctggtt
    30841 accgaaccga ctccattctg tctcaatgat ctgcagcttg ctgtggagac ggttcgcacc
    30901 ctcgggcttc ctttcggtgt agtgattaac cgtgcggacg tgggcgatgc cagggtgcgg
    30961 gattattgtg atgcagaaga tattccggta ttgctgacac ttcccgagga tcgccgtatt
    31021 gcagctgcct actccggagg tgccctgatc gttgatgcgt tgcctgaata cagagcatca
    31081 tttatggaat tgctcggaaa aattcgggac ggtgcggggc agcgtgagaa aggaaaggcg
    31141 gtgcgttcat gacttcccgt aaacattcct tcatctttgg tcctgtagta tcacgccgcc
    31201 ttggcagatc gctgggcgtg gatctgattc cggcgaaaac ctgcccctat gactgcatct
    31261 actgcgagca ggggcgcacc acctgccaca ccatgcagcg cgatgcctat gtggacgtgg
    31321 aagccgttct ggaggaagtg gctgcttttc ttgccacaca tccggcgccg gattacataa
    31381 cgatttcggg cgccggggag cctgccctgc atacgggact cggagagctg attcagaaaa
    31441 taaaatcact gacgtctgtt cctgttgcag tcattaccaa cggtgcgctc ttctggcagc
    31501 aggaagtgcg ggatgcgctc cttcatgcgg atgttgtttt accctcgctg gatgcgggaa
    31561 atgagcgtct ttttcagaaa atagaccggc ctgtcgcggg actttctttt gcaaaaatgc
    31621 tggaagggct gattgctttc cgaaatgagt tttccggtgc cctgtggctc gaagtctttc
    31681 taatggacgg tatcaatgat gatgaagaca gcgtgcgcga aattgcggca caggtgcggc
    31741 tgatccggcc ggataaagtg cagatcaata cggtggagcg tccgcccgcc atgagccgtg
    31801 taaaagcggt ttctttttcc gtgatggagc gctatgtttc tttttttgaa cctgaggcgg
    31861 aaattatttc gccccgttac attccggaca gcggcggcgt gctgctgcct caggaggcac
    31921 ctgtgctgga aatgctgagg cggcgtccct gtcccctgga ggagattgcg gaagggctgg
    31981 gttttcatcg gcttgaaacg gcgaagctgt tggacaggct ggtaaaagag gggaggctgt
    32041 gtgttatgga acgggaaaac aagctgtttt tcggtatagg ggaacgctat gagcaggcat
    32101 gatatccggg agctggtcat catcagcgga aaaggcggaa cgggcaagac aagccttacg
    32161 gccgcctttg cgtctcttgc cgggagggcg gtgctggcag actgtgacgt ggatgcggcc
    32221 gacctgcatt tgctgctgca cccggagccg gaagagcatt atgaattcag gagcgggcat
    32281 caggcggtaa tcaggcagga ggactgcacc cgatgcggag actgttatga acggtgccgc
    32341 tttgagggta tccggcggga ggatacggga gcggatgtcc ggtatacagt ggatccgctg
    32401 tcctgtgagg gctgtggcgt ctgtgttgac tggtgccctg ccgccgccat tgattttcct
    32461 gaatgtacgg caggggagtg gtatgtttct tcgacaccct gcgggcccat ggttcatgcg
    32521 aagctgggta ttgccgcgga aaactcgggc aagctggtgt cgacggtgcg ccgtgccgca
    32581 agagacagag cggaaaagga ggggattgca aaactgatta tcgacggtcc tcccggaatc
    32641 ggctgtccgg tgatcgcttc cattacaggc acatcgctcg cattgatcat cacggaaccg
    32701 acgttaagcg gacagcacga tctggagcgg gttgcccggc tggcgaagca ttttaatgtg
    32761 cccgtggcgg tgtgtgtcaa caagtgggat atcaattctg aattgacgga aagcattgaa
    32821 gcggtcgctc tttcaatggg cgcccgtccg gtcggacgga tcggttatga ccgccggatg
    32881 acggaggcgc aggttgcggg gaaaactgtt cttgattatg gcggtgccgc agctgaggcg
    32941 atccgtgaaa tctggtgccg ggtggcttca atgcttgacg gtacagcgcc tgatgccgga
    33001 gatgcagaaa gcggaaacgg cggctgatct tttttagata cgggacaaca agtcagatgg
    33061 aagaaaacta cagcgccaag gcattggaac gtttcagaaa tccaagaaat tatgatgaac
    33121 tgcaggagcc ggacggctac gccagaataa ccggtccctg cggagacacg atggagttct
    33181 gggcgcaggt cgatgatggt attgtgagtg ccgcttcgtt taccacgaca ggctgcggtc
    33241 cttcccgcgc ctgcggaagc atggcgacag aacttgcaga aggcaggccc gttgcggaag
    33301 caaaagaaat ctcacagcag gatattctga atgcgctggg cggtatgccg gaagcgcacc
    33361 ggcactgtgc actgctggca tcgaatacac tgcgtgctgc gctggaaaat tatcaggcat
    33421 cggcatcagt aaagcagatg aaaacagaag cataaggaag gtcgaggttg caccggatgt
    33481 gtcgcccgac tctgaaaaac aacaggagag aaaatgatgc gtgttgctat accggtagtt
    33541 caggggcatc ttgcccttca tttcgggcac tgtgcccagt ttgcgctcgt ggatacggat
    33601 ccggagcaga aaagcattct gaaagtggat gtggtcgatg cgccggagca tcagcccggg
    33661 ctgctgccgc gctggcttgc tgagcgcaat gcccaggtga ttattgccgg cggcatgggg
    33721 gcacgtgccc aggcgctttt tgccgagaac aatatcgaag tggttatcgg cgcaccggcc
    33781 gatccggtgg aagccctggt gcagcgctac ctggagggca ctctggaatc aggagagaat
    33841 gtctgcgatc attaaagcgt actgctttta cggccctgtt atctgagcat cttcagcaga
    33901 aaaggcagcg ccagtacgag ggctgccagt acaacaacgg caacgacaag catcctgttg
    33961 cgccgtttga cagacgggtt gccgatatct tcaagccgcg catgcttcgg caggacttcc
    34021 acggctgcat ccgggatatc gccctgagga gcggcaaccc gtggtgccac ttccagatga
    34081 ttgagcagtt cctgcgctct gactgattcg aagttcctga tcatgctgtg gcagagatat
    34141 ttggcttcat cgatgctgcc cagtttttcc agcgataaag cggctgcata catgatgttt
    34201 tccgtattgg gaaattctct gttcagctgc tgaagcagtt ccagcgcttt ttcatactgc
    34261 ctgtttttaa acagcctgtt ggcttcctga aatcgttgtt ttgcggattc tgaattcatg
    34321 gtgctttgtt ttcactacct gtttttttcg gcggactgat gcgttgccgg ataaatggta
    34381 ccatataaaa gcgtttcagg ggagaggcgt tgcgttcggc ttttccattt tttcccaggc
    34441 ttctgtcgcc tgttcttcgg gcacgggatt gttcacaaaa acatcatcca gaaaagaagc
    34501 gccggttttg aactgggcaa aactgagcga tgctttttgc gaaggggcgt tttcaccagg
    34561 gggtgtataa aatgccaggg tgacggtgtc tgcttcctgc cagacccgga ctgcaacggc
    34621 ctgctgctgg tcggaaccgg ggaaaacaaa gaggcttttt ttctcgtatg cggtaccgtg
    34681 ccgttcatcg agtgccttga agaagcggcg gcatcgggct tcaatgtctt ttgcatcatc
    34741 ggaccatacc gccaccagtt catagattct ggcatcctga aatccgatat tggcgaaggc
    34801 atgcacgccc agaaaagggt cggcgccata tgcgatcagc agtgcatccg gtttctgggg
    34861 gccgggagtg gtattgcgcg aggcggcgtc gctgtagacg gcatcgggca ggcgcttgag
    34921 gcattcttca aaagacatat tcaatcggac accgtgcagc atccggtcaa gatcattgtc
    34981 cggcgcttcg tctgccagtg cggtaccgaa aaagaaaaga gagcaaagga gcatggagaa
    35041 acagaagatg ggtttcatga aaagttcccg ggacagtcaa tcccattttt ttaaaaaggc
    35101 ggcaatctgt attttgactg tttccctgtc gtaatcataa aacaggatat gatcggaaag
    35161 gagcagccag tggagcgttt tatctttcga tggaatacgg ttatatacgg cttcggcata
    35221 ggccggatcc gcgacggtgt cattttttga atgaatcaga agggtcggca tggcgatctt
    35281 ctgcaaaatc gtgtcgtccc gtgctttgcc cgctactgcc atggcagtga gcgctccttt
    35341 cgaagggatc cagttatagg aaaggatttc gccccgtgcc tctgcacggt ttacaggctg
    35401 catgttttgc ggtctgtata cccagtgcag tatcggagag acaagtcctg cccaggtctc
    35461 cggacgcaat atataatacc attgtttcgt aacagcatag tagggcgctg tcaggatcag
    35521 cccgtccggc tgcttctgtg cagcggcaat ggttgcaagg gcggcgccca tggaatgccc
    35581 gaggatgacg acggtatcgt agcgctgctg cagcgcatcg agttctttca gcaccgcttc
    35641 ctccagcgct gcggggcttg tcttctgaaa atcgaaggga gaggtgccgt ggccgggcag
    35701 gagcatggcg cggacatgcc agccccgttc tgccacatac tccggaagat cggaaaagca
    35761 gtcgggtgtt gcgataaagc catgcacaaa aagaatggct ttccgggtgt catcagggcc
    35821 gcaggtgcgc gcttcggcgc cggaaagaat gccggtgtcg ggattcctgg gcagggtgcg
    35881 gtcatagcgt tccgcttctt tcggagcgta attattccag aacagaagag cgccgatcag
    35941 aagaaagggg ataaaaagga taacggcgat gcgaaataag gccattttta cctcctgttt
    36001 catgattccc atctggacag aaaacggctg accgattctt tcacctgatc ccggtcgtaa
    36061 tcccagaaaa cgacgtgatt ggagcgggta agccattctg cggttttgtc cggggacggg
    36121 atgacgtcgt acaccttttt cgatgctgcc ggatcggtga cagtgtcatt tactgaatgg
    36181 atcagcagcg tcggcatgtt tattttgcgc aaggtatctt catgccgtgc tttttcggcc
    36241 agagccatgg caacgggcac gcttttcagg gggatccatt tgtacagaat gatcttgcct
    36301 ttgtcagttg tttgccggat cggctgccgc gtgcccggcg cacagataaa gggcgtgacg
    36361 gatgaagcca ggcgtgccca ggtctccggc cgcatcatgt aataccaccg atgggtgact
    36421 gaataataag gtgcaacaag aatcagtcct ttcgggctgc tttgtgccgc cgcaatggtt
    36481 gcgagagcgc cccccatgga atggccgaca agcacaaccg ttgcatatcg ccgcttcaat
    36541 tgctccactt cctgcagcac ggcatcttcc agttcctgcc cggtgacctt ctgaaagtcg
    36601 aagggggaag tgccatgccc gggcagcagc atggcgcgga catgccagcc ccgtgccgcc
    36661 acatagtccg gaaggtcggc aaaactgttg ggcgtggcta taaagccgtg gatgaacagc
    36721 accgcccgct ctgcatcctg cgcaccgagg tgacgcggct ccgcccctga cagaatgcct
    36781 gaatccggat ggcggcgttg ttttttgtcg tggagccgtg ccagtctggt gctgataaaa
    36841 cccataacag ttcttttacg cttcgtcttc agtttatgaa aaagtgtgcg gctgcatcag
    36901 tagtataaat aagtggtgtc tctgaaatac agagcggaga tctcatcaga cttaaaaaaa
    36961 gcctccggcg aaaaccggag gcttgaaaca gacaggacat gttatttaaa agctgtcgga
    37021 agtgtccagc gtgccgtctg taggtccggc actgcggctc ttgatgtagg ggccggtcat
    37081 catatctttg tacgccattc cggggccgac catggggaag acgaaagaat ttttatcgac
    37141 gaacacttcg agaaaagcag ggccgtcgaa agagacaaag gctttcagtg cttcttccag
    37201 ttcgctgcgc tttgtcacac ggcgggcaaa tccgaagccg tccgcctccg ccgctttcac
    37261 aaaatctttg ctgtgcagcg ttttgtcggt gcccgagaag cggttgtcaa agtaaaggga
    37321 ctgccattga accaccattc cgtcgccgtg attgttcagc agcagaatct tcacgggcag
    37381 attgtaggtg gtgcaggttt ccatttcgcc gatattcatc cggatgctgc catctccgtc
    37441 cacgtcgatc accagcgcat cgggaaaggc agcctgtgct ccgatggcgg cgggcagacc
    37501 gaagcccatg gtacccatgc tgcccgatgt aagcagcgtt ctcggcttct tgaagtcgaa
    37561 gtactgggcg gcgaacatct gatgctggcc cacgccggtg gcgataatgg cgttgccttc
    37621 ggtaatctta ttgagttcct gcagcacttc ctgcggctgc acgatatcgc tttcccgatt
    37681 aaagttgaaa gcatgtgttt ctttcatctt ctgaacggcg gcgacccatt cgctgtgatc
    37741 cagggaaaat tctttcccgt attccagcag atcggacagt gccgttttga tatcgccggc
    37801 aaaagaccag tccacagctt tcactttacc gatctcggca gcatcaatat cgatctgggc
    37861 gatacgggca ttggcagcga atttatgcgg gatgccggca acgcggtcat caaagcgtgc
    37921 gcccaatgca atgagcagat cgcaatcgtc cacggcgtaa ttggcataag cggcaccgtg
    37981 catgccgagc atgtggaggc acagcgcatc ggtcgtatcc agagcgccta tacccatgag
    38041 cgttgtcacc gccggaatct ggtactgctt cataaagcac tgcagtactg ctgaagcgcc
    38101 ggaaatgacg gcaccgccgc ccacataaag cagcggcttt ttcgcctgct tcagcatttt
    38161 gaaaaagctc tccattttct tctcgggcat gcgccgtgcc gaaacggaat tggtgcgttc
    38221 ctgatagccg tgcagctgaa gcaccgactc gctgtcatag gcgccctgcc agttctgcac
    38281 gtctttgggc agatcgatga ccaccggacc gggcctgccg ctccgtgcaa tctgaaaagc
    38341 cgtacgcacg gtggctgcaa gctgcttggg atctttaacc agaaaaacat gcttggcaca
    38401 ggcgctcatg atgctgtaca ccggcgcttc ctgaaaggca tccgttccga tggcgttgcg
    38461 tgccacctgc ccgcaaataa caatgatggg tatggaatct gccatgcagt cccggacagg
    38521 cgtcacagta ttcgtggcgc cgggcccgga ggtaaccagt gccacgccca cattgccgct
    38581 ggcgcgggca taccctgccg ccatgaatcc ggcgccctgc tcattggcag gcacaatcag
    38641 gtttatctgc ttttccgggt tatttttatt gtattgaaag acggcatcat aagtggggag
    38701 aatggcgccg ccgctgtaac cgaagatggt gtccacacct tcttcggcaa gcacctgcag
    38761 cgtcatttcc gcaccgctca tgatctggcc gtgtgcgatt gactttttag ctttcagtcc
    38821 aaccatttta ttatctcctg cgaagcgtcg tccggacgcg gggggtgggt gggttgcttg
    38881 ggtaacaacg gctcttcctg ttttcttcct catcagaacc ggaggagcct gtgtgcttgt
    38941 gtataaaaag gcgagcgcca aataagattt gacgctcgcg gttcaattca gcttaagtga
    39001 ataagattag ccttcttgtt ccggttccgg cgccgcctct tcaacggtcg cctccggtac
    39061 acctgacagg gggggctcct ctgccggtgc gtcttctgcg gatgccgcct gtgcttccgc
    39121 ctgttcctgt gcggcagcat tctggctgcg gctcaccgct gcgtacagat cgtgcgccac
    39181 atcggcgaga cttctgcctg cctgatatat gccttgcggc agactgccgc ccagcgccgc
    39241 actgacgtcc acggactctc ctgcagggat ttcttcgcct gcttcgagcc gttcctgatc
    39301 gcgctgatat tcgcgcatgc tcagaccgat acggcgttcc gctgcgttga tgctgatgac
    39361 ttttgcccag accgtgtcgc cgactttcag aacatcttcg ggcttattca cacgttcctg
    39421 agaaagctcg ctcacatgga tcagtccttc aacgctgttg tccagacgcc cgaaggcacc
    39481 gaaagcaacc agtttgacaa tttccacttc gacatgagcg ccgatgggca gtttttccac
    39541 gacagacaac cagggatcgg actggagctg cttgagaccg acgctgattt tttccgcttc
    39601 gggatcaatg ctcagcacct gcacttcaac ttcctgaccc ttttgaagca cttcaccggg
    39661 gttggtgatt ttcttggtcc aggacagatc gctcacatgc agcagggcgt cgataccgtc
    39721 ttcaatctgg acaaaggcac cgtattcggt caggttgcgt acaacaccct tgatggcgga
    39781 gccgatggga taccggtcgc tcagctgttt ccagggattg ggctgggtct gtttgatgcc
    39841 cagagcaatc ttttcttctt cagcgtcaac gctcagcacc atcacgtcca cttcgtcgcc
    39901 gatgctaagc acttcgtttg gataacggac acggcgtgtc cagctcattt cgctgacatg
    39961 aaccatgcct tccacgcctt cttccagctg gacaaaggcg ccgtattcgg tcatgctgac
    40021 aacgcgtccg cgcatgatgg cgcccacagg gtaccggtcg agaaccgttt tccacggatt
    40081 ttccgtgcgc tgtttgagtc cgaggctgat ccgctcgctc acggggtcaa agctcagcac
    40141 aataacctga atcttatcac cgacctgcac catctgcgtg ggatgcttga ctctgcccca
    40201 gctcatgtcg gtcacatgca gcagaccatc gatgcccccg acgtccacaa aggcgccgaa
    40261 gtcggtaata tttttaactt caccttcgat aagcccgttg acttcgatgc gtttaagaag
    40321 atgctgtttt tcaatttcac gctgctcttc gagcagtctt ctgcggctga caaccacatt
    40381 gcggcgacgg cgggtcagct tcacaatctt gacttccatc ttttcgccga tgtagcgatc
    40441 aagatcgccc gtgggccgga aggtcagctg gctggcgggc atgaaggcat ccacaccgac
    40501 atccactttg agaccgccct tgacacggcg ggtgatgacg ccttcaataa taccgtcgtt
    40561 gtcgtatacg gcctgcacat gctcccagtt tttgatccgg tccgctttaa tcttggagag
    40621 attgggggcg ccgtgatcat tttccggagt ttcgatataa aaatcgtatg cggtaccgat
    40681 ggcggtgctt tcgccttctg caaattctcc caggtcaact acgccttcgc ttttgtagcc
    40741 gatatccacg gaaacgcggt tatcgttgat gccgacaatg cggccgcgga cgacttcgcc
    40801 ttctttgaaa ttctgaggcg cttcctccat cagctgcgac atgctttcct gactgatttg
    40861 ctcgttcaga tcggcctcca ggtcggcagg cataaggtct gcgccgcccc gggccagttg
    40921 ggacaccaga tttttcatga aactacaaga ctcctaaaag gtgggtggaa ctccccgcga
    40981 catacgggtt aatatgctat tttaacaaaa agtgtggtat actttcaatt tacagcaggc
    41041 gaggactgtt ttttcctgga agcaccggta tgggattatt ctaaattggg attgctcgaa
    41101 atcggaatac tcggactggg gttgtccatt gatgccttcg ctgttgccat ggttgccagt
    41161 acacttctgg tgacggtaaa caggcggcag tttttcagac tgtcctttca tttcggtttg
    41221 tttcaggcgc tcatgcctct catcggatgg cttgccgggc agaccgttga aaaatggatt
    41281 catccctggg atcactgggt gatctttctg attctgcttg ccgtgggcgg aaaaatgagc
    41341 tgggaggcat ggagcaatta cagaaacggt accgatcccc tggcttcctg ctccaccgac
    41401 ccgacccggg ggctccgcct gatcgggctg tccatagcga ccagtctgga tgctctggcg
    41461 gtggggctga ctctggcggt catgcaggtg aatatctttt tcgtcgtgtt tgtcatcggc
    41521 tgcattacgg catccctcac ttttacagga atgaagctgg gcagccggct gggacttcat
    41581 ttcggacaga aggtgcagat attcggcggc tttctgcttg ctgcgctcgg catgaaagtg
    41641 ctgcttgccc actttttgag ctgagccttc ccgcttccga agccctccga tttgcaatat
    41701 cccgccctgt tccggaaaat aaagaggcag gcagaacacg ctgcggaaaa accgctgaaa
    41761 gggatcaggc ctatggatat ccagtctctg atggctcctt tttcttttgc agaaaaagaa
    41821 ccctatgcca aagccatgga agcagccgct tcaggaaagc cggtggcagg gtatctctgc
    41881 tcttatgcac cgctcgaact gcttcatgcg gcggggtatc tgcctgtccg tgttttcagc
    41941 cgtataggcg acacacacca cgcggatgag ctgttgcaga ccttctcatg cagttatgcc
    42001 cgcagtgtgc ttgaccgtgc tctgaaaaaa gaatggccct ttctcgatat tgtcgtgttt
    42061 tcccacacct gcgataccat gcagaacgtg gcagatctgt ggcgcgcaca tcggggcgat
    42121 gcggaggtgc tgattgtgtc ggtgccgacg cagacggacg gggctctcgc agaaaagtac
    42181 tacggtgctg agcttgcccg tgtgcggtgt ctgctcgaag aaaaaacagg tcccattgcg
    42241 gaggcgcgta ttgaagaagc gctcgcactt tttcagcagc agcgcaatgc catggagcag
    42301 ctgtacgcac tccgcgcacg ttacccgaag gtactcaccg gagcccgggt tatgcagatt
    42361 gctcttgcgg cgcagctgat ggacagggaa gcgcatctgg cagcgcttca cgcccttctt
    42421 tccgccgtgg aatccgcaga ggaggcggct tcagacgata gcgccgccct tccgaaggtg
    42481 tttgttgcgg gcagcatgtg cagtccgccc gccttcatcg atcttattga agaggcaggc
    42541 tgccacattg tcggcgatga tctgtgtgtg agtcatcgcg ctttttacag tgccgatgcg
    42601 gaagacggca gtacgctgga ggtgcttacc cggcgcagcc tgcaccgtga tccctgccct
    42661 gcttttcacc gaacgggctg gcatccgggg catgcgctgt tggcgcgggt gcgtgcagca
    42721 ggtgcggatg gtgtgatctt tctgctgaca cagttctgcg accctatggc ttttgattat
    42781 gtgcccatga tgaaagccct ggaagaggcg gggattcccg cgattacgct ttccgtggaa
    42841 caaaatcagg aaccgtcaga gcagctgcgc acccgggtga gtgcattttc agaaatgctg
    42901 cagggatccg catgacatga atacacatac tttttttgcc ggggtcgatg tgggcagtgc
    42961 cgctgtcaag gtggtgcttc ttgacgaagc agatcagatc ctgtctcagg cggtactgcc
    43021 gaccagcggc gtgctggccg attctttccg ccgtgcgctg gatcaggcgg tggacagtgc
    43081 cggtttgcac gcccaatact gccgcggact cattgcaacc gggtacgggc gggatcgtgt
    43141 ggaagggcgc atacggtcgg taacggaaat tacatgccat gcccggggtg cccgcttctt
    43201 ttaccctgag gcgcgctcgg taattgatat cggcgggcag gactcgaaag ccattgcact
    43261 ggatgagggc gggagggtgc tccgttttga aatgaacgac aaatgcgccg ccggtacagg
    43321 ccgatttctg gaagtcatgg cacggctcct cgaaatagaa ctggatcaat tcggaggccg
    43381 tgcactggcg gcggatgcgc ctgttgccat ttcctctacc tgcactgttt ttgccgagag
    43441 cgaagtgatt tcctggctgg cgcagaacca gcctgtgaat aatattgctg ccgggctgtg
    43501 ccgctccatt gcttcgcgcg ttttcgggct tgcttcccgt gtccgcatga cagggcccac
    43561 cattatgacg ggcggtgttg cccggaaccc cggtgttgtg catgcgctgg aggaactgct
    43621 gggtgagccg ctgctccttc ccgggcagcc acagctggca ggtgccctcg gtgccgctct
    43681 ttttgcaaaa gacagcgtgc tttcctccgg cgggcaataa aaaacgcccg ccattgatac
    43741 agcgggcgtt gaaaaaccgg aggtgtcgtt tttatttggc gtgctttccg agatagctgg
    43801 cgacgccatc ggccgtggct ttcatgcctt cttccccttt gcgccagttg gcggggcaga
    43861 cctcgccgtt ttcttcatgg aactgcagcg catccagcac ccggatcgct tcgtccacgc
    43921 tgcggcccag aggcaggtcg ttgatcaggg cgtgccggac gatgccctct ttatcaatga
    43981 ggaacagtcc gcgcagtgct actgactcat cgaagagcac gccgtagtcg cgggcaatct
    44041 gcttggtcag atcggagacc agcggatact ggatattgcc gataccgcct ttgtccaccg
    44101 gggtgttttt ccacgcccag tgagagtaat gggagtctac ggaaacgccg atcacttcac
    44161 agttccgctc tttgaattcc ttcagctttt tatcaaaagc gatgatttca gagggacata
    44221 caaaggtaaa gtccagcgga tagaagaaaa gaaccacata cttgccaagg taattggcga
    44281 gccgaaactt ctcattgaag gtgttgtcgg gcatgactgc tgttgccgta aaatcaggtg
    44341 ctgttttggt gacttccaat gccatggaga tactccttat tttttgagat gagggttgcg
    44401 gtctgcctcc cgttcgaaga acagaccgga taaacacgca ttcagtgcag ttatgccgga
    44461 aaacgccggc gaaggctctt ttatttcccg gagcaggagt gtgcgcccca caaacggttc
    44521 catgccgttc tttttatacg aagccccggt gcatcccggt gcctcgggta catctgaaaa
    44581 aaaagccccg gcaggcatat ggtgtcacgt gcgcacctat acatgccgga gctcattttt
    44641 cgaagcaccg gatccgggcg aagtgttttc gcttcaccct gatccgggct gttctcgatc
    44701 aaagaaagac aggtctgctg actctatcgt aaatgtcttt tatttgcggc gtgaccgtgc
    44761 cagaccgatg cccgccagag cggcagacag agcaaccagg gcgaccgcac cggctgcagg
    44821 aacggtagtt tctttacccc gtgaaagaac cagattcacg gcagtaccgg gggcgacaga
    44881 agagcccggg gctacgctct gctgaagcac ctgacctgca ggggccgtat cgctgtacac
    44941 ctgagaaaca ctgccgacaa ccagcccggc ggctgcgagg aggtcggcgg cttctgcctc
    45001 agtcttgccg acaacattgg gaacgattga gggctccgct gcaaccacca gattgattgt
    45061 gctgccggca ggcagttcca atccggcggc aggatcctga ttgattatct gacctgctgg
    45121 agccgcaccg ctgtaatcat aggtgacggt acc
//
LOCUS       DAOPWB010000008        41314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_885_length_41314_cov_5.182990, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000008 DAOPWB010000000
VERSION     DAOPWB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 41314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41314
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_885_length_41314_cov_5.182990"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKN92_01735"
     CDS             complement(<1..365)
                     /locus_tag="PKN92_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47543.1"
                     /translation="MKYRNFFYGNALLICLLLATPAFAWGPRSQQVIVTTALQLVSRE
                     QNLPLNRLQSDIQAGAALSPPMIEALYPDLLSDPLRAIENEMALLTAARGAKIDSYYA
                     FRLGALGKVVSNYAAPMYTA"
     gene            complement(397..1950)
                     /locus_tag="PKN92_01740"
     CDS             complement(397..1950)
                     /locus_tag="PKN92_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47544.1"
                     /translation="MAGSKNCPYCGGTVNQGATVCGQCGGPLKKAPAGAGSNPLKVTH
                     CPQCNTPVSPGDILCMSCGTNLLTGQKVAAPKAQASPESGRSLWVVAAKWVSAALLVF
                     AAIGIALFVTLYLLRDPVGEALKYARSEDWDHAVSTLKNYIQKNPDKTNAQFLLAKFY
                     WKMQEYDKAYDLFALVAQKGESTARDALLLAVLAAEEIADKQPEYREKQRSLLSDLAG
                     TRFAGDGEVQLLLALTLGKEGDFARQKGVMDKALASGAKPADILPVLAYALDRDLPAL
                     EQFVERQQQAGSTLNADALAAAGIMRLEEGQSDSAIATLSTLPSVSPHVDGWVKLQLG
                     ALYMGKGEYGKALPLLSDAKHQQPENERAVFLHALCLQENKLTEEAVTAFDQLVSARK
                     NYAGQAALQLAQILSDQGNLDRAAAMAREAAELGLNTARQAAIQGMIFARQGDSMQAE
                     QAYRRALSMSSDYAPAFLELGLLLIARNATDEGLAQLDQYLKLAQSDPVGLHANEIEL
                     LVAQIRQSQ"
     gene            complement(2157..2687)
                     /locus_tag="PKN92_01745"
     CDS             complement(2157..2687)
                     /locus_tag="PKN92_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47545.1"
                     /translation="MEFYIDLFRPLFPHFQEYSYYYIFGVPVLLLLIYITRWYSVPTI
                     LYVLEYTLYAGIMHTVVHVLVRLFCWFKNNSSMNALQADGTRADPEAWTTPWKQFWNW
                     EYYDPEWIPWMELFFLIVVAGLMIRFRPFRPTKRRRRAYVPPPPPKPSAADDNWGVPK
                     QYHVPAASSAAHKKKK"
     gene            2834..4195
                     /locus_tag="PKN92_01750"
     CDS             2834..4195
                     /locus_tag="PKN92_01750"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNZ47546.1"
                     /translation="MKKILFKILVVTIGALIVLIAGTLLWLNPGRGPAEAVDPHAKGW
                     AEEMEGTLVLHLAGTPYEMGYQRGYFAREKVLLTLSLFENLLELAQEEMGLPRFASHL
                     ILDLTYQLCSPYIPDRFKRELEGIADASGCDLKMLRRGHVISVLTERGCSAFAVWGKA
                     TKEGKLFHGRNFDWILSAGLEDTAVLCLYEPEGFHRFASAGYAGVIGVLSGMNMEGIS
                     ISQIGAITKDKSLRGLPLEFVLRRILEETSNLDEADALMHSVKHTVGFNYVVADGDAG
                     DARAFETTAHHIATFGPGDPKETVEYAIPIEDAVFRSDEAMDPLIRSLQECANAPGLP
                     YGSNSYDHRYLGIAKRVQERYGTIDADYALEILKATAMDNANLHSVLANSSDRELWIA
                     HAKKGQQASLQPYIHYDLKQLFLRPEERNPVAPPPETHSETAAETDTPTGNTEAATAE
                     ASS"
     gene            4286..4615
                     /locus_tag="PKN92_01755"
     CDS             4286..4615
                     /locus_tag="PKN92_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNZ47547.1"
                     /translation="MLKGLGDLGKMGGIIKQALEMKGKIEELKEKMGDMEIPYAIGGD
                     MVTVVMTGKLEVKSLTISQELLEQNDREMLESMVQAAMSGAIAKAQDAVKEKMTELTG
                     GIDIPGL"
     gene            4618..5553
                     /gene="fmt"
                     /locus_tag="PKN92_01760"
     CDS             4618..5553
                     /gene="fmt"
                     /locus_tag="PKN92_01760"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNZ47548.1"
                     /translation="MRIVFFGTAEIAVPCLASLCTAHEIAAVVTQPDRPKGRSKKPEP
                     PEVKVWALEHGLEVVQPEKLNDGNFERWLREKKPELCALAAYGRLLKQPILDVPLRGW
                     LNMHPSLLPRWRGPSPVQTALLAGDDVTGVTIMRITLEMDAGNIVLQEKLPVGRTETA
                     GELSRRAADLGGRLMAQAVAQMETGKLTEYPQEHDQATFSRMLQKEDGIIRWDREAAS
                     IHNQIRACNPWPVAHCLFRDRMLRLLRSDYSEKSHSALPGTVIQADRQGIVVATGKGC
                     LTITELQWAGKNPLAVDAFLRGTPVRPGDCFQEVT"
     gene            5553..6887
                     /gene="rsmB"
                     /locus_tag="PKN92_01765"
     CDS             5553..6887
                     /gene="rsmB"
                     /locus_tag="PKN92_01765"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNZ47549.1"
                     /translation="MAVDPVRDAAVSVLLRVFQTEAHNDVSIDRTLRRTNLSPRGARF
                     MTHLVYGVVRHRLLCDHVLQPLCDQPLDQLPLSVLIVLRMGVFQSFFCSNVTRPALVH
                     TGVDLAKKRGHSGLAKVANAVLRRLPSSLDEITLPDRTTQLMEHLRIRYSMPRWLVRL
                     WCELYGEDGAEAYCAACDSAALPSVRINTLLAQTDDVLQQMQKAGIAAERITEALDAL
                     LLHDGTNPQATRWFRQGQLMQQDPASILAGALVDPKPGEKILDMCAAPGGKTLHLCAL
                     SRDAAPIFALERYAGRVARIVENRERLGGTQVLPLCGDGLQPPFQDGTFDRVLVDAPC
                     SGLGTLRRHPEIKWRIQPETPSLLAATQKAMLRKALQLCKNGGLVVYSVCTPTPQETT
                     EVTAAILAEGGCMPEDGPEQFDSWKTALGQYQTSPKNGAWDAFYLMRFRKQS"
     gene            6845..7918
                     /locus_tag="PKN92_01770"
     CDS             6845..7918
                     /locus_tag="PKN92_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNZ47550.1"
                     /translation="MGRFLLDAFSKTILSSVFFIIRFFVWTLQWSFAVVLFLLVMAGT
                     GYYVYNVTLKGRAHVVVPNIVDRPISEAAFLLSEKHLEIGKQVQATHPSIPKYYVIAQ
                     RPAAGRVAREGRRVNVIVSMGEDYMRAPDLTGKNLEEARREILASRFRVGSLARIPEE
                     QPRDTVLSQDPPAGARLPEQGEIHLLVSAGSGRPSALMPDLRGLAISDIARAMEGFDV
                     VLVANAVDIPGAVPDIVLAQSPPPDTLIYQNQVVTYDYLRSSGEKAPTQQYEDTVRHQ
                     MFYDWYSRDVRVDVVDRLGNRQTIWTKKPLFDEEALRTYVTGTVIRVPVTYIDEVSVE
                     VYIDNELEASYTLKEGRPPVKIR"
     gene            8016..8672
                     /gene="rpe"
                     /locus_tag="PKN92_01775"
     CDS             8016..8672
                     /gene="rpe"
                     /locus_tag="PKN92_01775"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNZ47551.1"
                     /translation="MSANTIKISPSLLSCDFSRLGEEIQAITDAGADMIHCDIMDGHF
                     VPNITFGPPVLASLRAHCRLPLDVHLMIEEPENYVQAFVEAGADILTIQVESTRHPHR
                     LIRRIQDLGCKAGIVLNPGTPEDSIKYLADMVDMVLVMSVNPGFGGQSFIREMLPKIS
                     RIRDMIGDCDLQVDGGIDEHVAADVIAAGANVLVAGSYIFNHHSYAKAIRLLKTAHHT
                     "
     gene            complement(8681..9958)
                     /locus_tag="PKN92_01780"
     CDS             complement(8681..9958)
                     /locus_tag="PKN92_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNZ47552.1"
                     /translation="MSLKERVEKAHSEMKHHLTEELLPFWSTRGVDKEYGGFLTYLDK
                     NGDPTGEGVKTLVCQTRMIYTFSSISRAGLDNGTFLPLAKQGVDFLLKHFKDRENGGW
                     YWTSERDGTPLDRAKIMYGHSFAVYALSEYAMAAQDSAVLQHAVDAYDIIKTRSADLQ
                     YGGFGEFFEEDWSPKKPGVYGGDRKSLDVHMHLMEAFTNLYEATRNHRHKRDTVRVIE
                     LIFERMIHPVYGTGIAQFSFDWTPLRAILFKNVWGSDRDVEDDVGRPMNNTSFGHNVE
                     FAWLLMHSLRILGLDVDPFKERMRKLYDHCHSYGIDWERGGVFCEGPGDGPARERNKE
                     FWQQAETLVGMLDAFLLFGEEKYWNAYENVHRFVMDRVINHEVGEWYPLFDEHNNLLW
                     DYMGHAWKINYHTVRAAIQCEQRLSAILKKMGS"
     gene            complement(9988..10566)
                     /locus_tag="PKN92_01785"
     CDS             complement(9988..10566)
                     /locus_tag="PKN92_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47553.1"
                     /translation="MTTKGQRFALPFFALKFMVFVVFFVVVWWECLPYYGQALLQITG
                     LPLKYALGVPIDSGFIDVQGKLNTESSLVFSVSGHERRMKIALLATNVPPYIALVLAT
                     AGLTWRRRGLILLYGCGILCFFHAFFIIIAMRFQDKLMLISEVPTALVQFFLTLPFML
                     WIVFAYWEKLMTLFQDKASGTEKQLPSEKEQS"
     gene            complement(10563..11471)
                     /locus_tag="PKN92_01790"
     CDS             complement(10563..11471)
                     /locus_tag="PKN92_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HNZ47554.1"
                     /translation="MTQNQESSETLSEKKRQSRENRKRIFLFVAVFVITTLVLLTSYR
                     YLIPTRINDWYLFQAARHTAWVLDRIGHGEVEPLHYSRLDIKGTRAALQAWKKGKESL
                     SSEETALLSDEPLSAWERWSWRAVTTRKQDARRVNGPRVYFVFREGISSRINSLSGKI
                     NQLEAQPDSDAPALQSELEHLKNDVQALRQEQQEGKEDLSLVFPFILVPECGAIEIMA
                     IFVAAILAFPTCWRKRVIGLAAGIPVMYGVNIFRLTVLAIIGAVDQSRVWFNLAHEYV
                     WQAVYIIFVVAVWLVWVEYVVKRVRS"
     gene            11677..12864
                     /locus_tag="PKN92_01795"
     CDS             11677..12864
                     /locus_tag="PKN92_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47555.1"
                     /translation="MRKNDIWAVLDIETSRSIPPAAIVQISCQRMRGWRADGAPFRML
                     LNQRVVIDPDAEAKHGFSREYLEEYGFAPGYAYRKLRDYAEDCPTVMSELALNWKHFL
                     EPDMARTGFSELPPPRFCLTALAERLIPETGEHKAETLLALFQTETDLEKTQLSPDTP
                     LWVILLRAVLRKRLNALGITGFEQIETFSKSFPVEDSKKAVREALEAPEQWYVLTSGQ
                     LPKGPYRLRHIVAMSKGDNWLVARKGAEQWTPLRHLPEFRQAQEANEYIQAISGKGDK
                     PATKPILLGSREAVSSAPLFPSLKAALKKSTASEDITDSAQDAVKVLVDILREIDSLN
                     EAGEAAMQSLRLQLAQCPDPHLFPLPYLSKVLESFPHDRKLQPEERQELLASIREILS
                     LLP"
     gene            13039..13728
                     /locus_tag="PKN92_01800"
     CDS             13039..13728
                     /locus_tag="PKN92_01800"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNZ47556.1"
                     /translation="MNNQSNTHKEERASASHLRIVSQNGYPTDWSALDDDSLMAKHQQ
                     GEERAFEELVNRYQRSIFSYVYRMLQNRHIAEEITQEVFLALVRNSDRYKAQGRLSSY
                     LFAIASNKVYKEWEQRRRRPHFLSLSFWSSDNGFSDGGLTPLDKLDDKQARVDKACEH
                     MEISEAINDALRHLPAHYREAFILRRFQDLPYDEIAEILDCPVGTVKSRVARAEKALR
                     PYLEKFRDYIE"
     gene            13745..14431
                     /locus_tag="PKN92_01805"
     CDS             13745..14431
                     /locus_tag="PKN92_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47557.1"
                     /translation="MSSDSPILRHPSSIQLMEYAESLVDNDAPVCALIASHVSRCPEC
                     AAEVRAIRHTLKVSTLSQIPEAENSLVQAILLQARKEAAADSAKTERNRWFPVLRGAA
                     CFAGIILLGMLSFSAALHEGSKAPSIALQAEARPIPAPENGWNPVRPFHGSVVQALAA
                     SVAGSGDGLSRSADAGYRRMVDFLDQDLDAATAALQRNPGCVRATQILDAEAKKRLEG
                     LRTLFIDRTL"
     gene            14471..16363
                     /locus_tag="PKN92_01810"
     CDS             14471..16363
                     /locus_tag="PKN92_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNZ47558.1"
                     /translation="MGRILLSLLLPGLLLTASPSSAQLIQHLQSGIEEVARGMDYVGR
                     RAGTLFSGTEGKNTANDTLPSVQREVEEHYPATPASTLFLANEFGDIRIRCWDERLIQ
                     IKAVIQVSAPTEDLAEQVSSLIELRVEQEGDYWECRTSLPELRSGDAVAMSVHYEAAI
                     PKDATLTINNYFGDVYVNDLGGALTAEIQYGALHLSHIQGLVRARIQGEFPVRANYLN
                     QGGTFIMTGTDAEFSNFQGNLDITQFRGSVTVRHPGPESTVNLISENGRTEILLPPDA
                     NPDLTATVAHGRFESELDVTRVVRGKQLLARHLNAEAKQRFMVNASFSDVLVGIEGGM
                     SQTVSRMSADQKAFTDTTTTLIPFESGNTMVIDAMPGNIHIEGVDEDQLSVEMTRVAW
                     TPSAAAAMDALDALEIKTVKTPGFIHLTTRATQDMTAFSCQSYRADLRIKCPRTLPVT
                     LNAEKGATVLQGVGADCVVNQESGEVQFEHMRGMLTVSGNTTSVTAKSCIGPLIINVA
                     SGDITAKEIFDSISINNGEGRVLVDTPGKDTEVRQRKGDVHFLSLAPLAGKMDIFTEA
                     GDINVFIAPESSASITVKTQGGQIRSALPLTGSINLESQEFFGRINTGEHTVLLETVQ
                     GSIYLN"
     gene            16404..16859
                     /locus_tag="PKN92_01815"
     CDS             16404..16859
                     /locus_tag="PKN92_01815"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47559.1"
                     /translation="MAGRRIAMRMFLFGCMGLVFEIFYIALHDAVFGGSWNLRGHSSL
                     WMIPVYALPGLLTGPVSHRLKNRHIPLPFRAFIYMLMIFAVEYVSGVLFTAAGLDIWN
                     YSNLRWNLHGHIALSYVPVWYFLALWLEYLYRLIDGCACVLVKSHKTTQ"
     gene            16953..17600
                     /locus_tag="PKN92_01820"
     CDS             16953..17600
                     /locus_tag="PKN92_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47560.1"
                     /translation="MEDMHLLLDKLGDKEAAWVERRDAAESIGKELQQILGRLTNHLH
                     DEDMDVKMAVERSLDPVHLLLRTRDKGNNEFSLRDLAFFCEKTGVRSVKPYKAGFVIR
                     VRLPHGNQQSVYLLPHQLRDGRQTVRVLAICCEANSHTVRKALRTNSKLAHSAFSMTR
                     SKNSVYISLLSNVFRQEATPDRIKRAVKEVAYYADWMAHLNAPSEKDEESEFDQD"
     gene            17624..18142
                     /locus_tag="PKN92_01825"
     CDS             17624..18142
                     /locus_tag="PKN92_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNZ47561.1"
                     /translation="MMKPHETPDVVAGVGACCAGESIGPEDAGSLDFHEKDLSGQCFS
                     GASLVGANFRGALLRGTRFDGADLSNADFTGADLELAVLGGAVLRNASFHSANLERAN
                     LVGVDAAGADFSGARMYFSRPGNGNFTGASFQGAQLDRAIFRRATLVNCDLRGATGVP
                     NFENARLDGVIK"
     gene            18139..18699
                     /locus_tag="PKN92_01830"
     CDS             18139..18699
                     /locus_tag="PKN92_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ47562.1"
                     /translation="MIFAHFRTEINESNQFVIACSQEKQALLIDAPVLDKRIPAFFEA
                     HGLSLAGIFITHTHYDHVEGLADILQWRQAPVYAGKSSIAGILAEKKKQDDPLDLGCL
                     KGRFIALPGHTACSLGLVIGERVFTGDALFAGSIGGTPDDITKQQEIQAVQQQIFTLP
                     HHYQVHTGHGPSSTVWIEKTFNPFFV"
     gene            complement(18702..19589)
                     /gene="metF"
                     /locus_tag="PKN92_01835"
     CDS             complement(18702..19589)
                     /gene="metF"
                     /locus_tag="PKN92_01835"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNZ47563.1"
                     /translation="MTKFRTFYDTGQPVISFELFPPKTQEGLENLFLHLKELAACKPA
                     YFTCTYGAGGSSRERTLGVLREIHDTYPQIPLASHLTCVGSSVDELRAFLRQAQEIGI
                     THIVALRGDLPQEDPEYTPPADGLRYANELVSLIRSEFPAFGISVAGYPETHPEAPST
                     AADIENLKRKVDAGADFIVTQIFFDNADYFRFRDRCSDAGITVPIIPGVLPVTNLTQT
                     RRIVNMCGAHLTSKLVKRLEVYEDDHEGQYAVGVYYAARQAEELIKQGAPGIHFYVLN
                     KSRATVQICRALALFTPVD"
     gene            complement(19643..21421)
                     /gene="menD"
                     /locus_tag="PKN92_01840"
     CDS             complement(19643..21421)
                     /gene="menD"
                     /locus_tag="PKN92_01840"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HNZ47564.1"
                     /translation="MNHNGNMNMLWATLLIEEVTRLGIKCFVIAPGSRSAPLAVAAWR
                     NPSVKTRVHYDERGAAFFALGAARAGVPAAVICTSGTAVANCFPAVAEANLSGLPLVI
                     LSADRPPEMHQCGANQTMEQAGLFGSHVRASLSLPCPDAGIPAGLFLSRIDQALSHCF
                     AQAPGPVHINCPFREPLAPVPVPASGQEDYLDELKEWHAGTVPWCDHADSDKKPDPEE
                     IRAVLESFAGKKNKALLLGAMHRQEDQEMACRLAKRLGWRVLPDILSNCRRNETGLSF
                     LSGYDLLAKAGCLGHFFQADAILHLGDTFVSKALYEFLQASPPELYVHVHPRSDRRDP
                     AHLVTRHLQGSIAFVCRSILNMPEIDRLRAEAETLPTEGLFSRAVEILNDLTDTAPLT
                     ELSLFLLLHERITEGTAFFSGNSMPIRYMDMLDQPCCAFLIHANRGVSGIDGNIATAA
                     GIAFGCERPTVACIGDMTALHDLNSLALVAASPTPVILVIVNNGGGGIFHHLPIAAFP
                     EVLVPCFVHPHRREFSGAAAMFGLPWYAPETVGAFKTCWSAVQDAGESCIIECKVDKE
                     ISLQAYSRMIDGIRKEYAPGETTAES"
     gene            complement(21411..22799)
                     /locus_tag="PKN92_01845"
     CDS             complement(21411..22799)
                     /locus_tag="PKN92_01845"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HNZ47565.1"
                     /translation="MSELLETDRLKQQETACSCLRRFLSLVKETPLSSSGALVRLQIE
                     APDLDALTWLGSLTGVPRYYWRDRKGTVEMAGVGEADVLDAQGGRDIEQVFALMQDRL
                     KNGSEGIRYYGGFRFHSSPARHGLWRDFQAHRFVIPLLEFCRENEKAVFAAAVKDDGG
                     RRKLIALLERMISAKQAVLPELPVFSGRSDLPDFSGWKKQIEDALHAFADGPLTKVVL
                     ARESSFHASAAVDPIALMCLLADTAPDTTLFCFQPASCRAFLGASPERLYHRRGVHMQ
                     TEALAGTRPRGLDPAEDARLEKELVCSKKEQQEHAIVVESLLTTLDNFAVELQYPGKP
                     AIKKLAHCQHLYTPVRVRLEQGVSDSFLLRQMHPTPAVGGYPKKEALQYLREHEPFER
                     GIFAAPVGWIARDSADFFVGIRSAAVLGNRLCLYAGAGIVPDSVAEEEWRELDAKIHQ
                     FAGVIEGRMHES"
     gene            23073..24074
                     /locus_tag="PKN92_01850"
     CDS             23073..24074
                     /locus_tag="PKN92_01850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ47566.1"
                     /translation="MAHTFCATLRHHKHIPVLMFLFLTGLLLRASWLMEIQRTPDFQI
                     PLLYQVDMGFIDNSALDYAQALRAFFHLPDIELKFERQIVWRHHAGDPLLRPPGYTFF
                     LSLLYFLFGDNQLLVRIVQMSLGMAGGLLGFLAGRKLAGVRAGVIAAGALWLYWPLIL
                     YESVLHEPVLVVFVSLLFLNSALQWLKTTGLKQAAKTGFLCGIFTVCASQIILFLPVM
                     LLWFLWFGCKRFRNLRRSLSHAGLAGLFFFLPLLPVSCLNYYESGMFVLNSHGHGITL
                     YIGNQPGAEGLLMPGKPLAEEFLGLQDSGLRADEIETLFRTGLPGLIFLKRRQSGRY"
     gene            24446..25222
                     /locus_tag="PKN92_01855"
     CDS             24446..25222
                     /locus_tag="PKN92_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47567.1"
                     /translation="MTRFLADIKGAQWRRAALNGLGASVFILPAFLFHWNYDQDIQTW
                     LYHHVKVYENQGNSEAAAALALFASEKAPDHAFVQKTAGDLLLDSGRKEEAMIHYAKA
                     RKCTQGVIDQTELLAQMGIIQRDSGNNDAAELLFQEVLQHNPEHSTALHFLGNIAYEK
                     GAYETAETLLKKAVALETTQGNTFFLLGLTQRAQGKTEEAERAFRQGLFFDPDNLWLQ
                     LELADVLTLLGHKEEACALYQKVLENNPGNERAQNALSAP"
     gene            25542..26993
                     /locus_tag="PKN92_01860"
     CDS             25542..26993
                     /locus_tag="PKN92_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ47568.1"
                     /translation="MRILFVLNIPYAIEPHGVMLLSSICKNAGHEVQLILLKKDSLIK
                     RVQEFNPHVVAYSVIGSEIDIFAAADEKLRRYCEESGTRIFRIMGGPHPTFSPGILDQ
                     MSLDAICQGDGEKALPALLECLEKNEPLDDIPNIALTSAGARLREKLSSEELDAQPFA
                     DRDLYYAAVPYIPRTGLRSFVASRGCPFHCTFCYNHVYNKMFEGCGPILRRRSVNSLL
                     TEIEQVIAQYPPVRFIRFFDDTFSLTVDDWLREFAEEYPKRIGIPFYCLMRPNTFSEE
                     AAVLLKKAGCYSLSMAVEAGSEPIRNTILKRGLSDKDMRTAFERARRLGINTYGNTMV
                     AVPGTTIEDDFHSMEFTRSLKLTVPTFSVCSPSRGTELADFAMEQGLMPADADMITRF
                     SAESPLNSYTEKEKKIQARIACLGTLYCTVPGFLKPLAKLMITSPFPIGLARKIGFSF
                     AIFRIATRLFPHAIPKNPITIVRVAIDGIRYFW"
     gene            complement(27012..29294)
                     /locus_tag="PKN92_01865"
     CDS             complement(27012..29294)
                     /locus_tag="PKN92_01865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ47569.1"
                     /translation="MKKKYCFEALLVLVACILSFFICYVYGVSLEDPLDHAAPMAMNL
                     FFPSVFFAAGHGLGTADASDIPGLEDFLLRNTDHFDIQNIPSETRTLPVSSPFELTHL
                     YTLYLVGWFWRFFGISVHSMLLCSAFLSALAGVALFGIFRLGLRRGYSLAGVFLVVSS
                     PVFLYTSNNLRDFAKTPFVLLALWMALFLFLKPAGLRKYLFFSLGLGIVLGMAMGIRQ
                     DVAMIVPPLALLFLVGTKFKSNHSFFWRLAAGLLFVVPFYLLSAPARYGAALEGNQAF
                     THSFFTGVSPDIEEKMSFGCASYDALFWTDAGLYAQANVLARRRGDTSSMANVYTAEY
                     RQEQREKDAPRMINPNLYYTSAKYGEYGQILFLDYLKHFPADIVYRAWVSTSAFYSMP
                     LEVCMMTKQVRASFPLLIQKIFTGHTLAAHGIRYGGLCLVLLSLLVLSARNLKIAYGL
                     AFLILWCTGYVSVCFDTRHMAYLSFVPFLVLLAGLEWVHNCAVSGWQALRKKQHPDQI
                     HNHFPCSEARCIFGSGEMLKKILLFSLTLCLAVVVPLVPLRLWQKQKVHSLLDIAESL
                     KRYPVSVTAEDVQGNESSMLVRPSEPLPGLPDEDFHAPGEASWEYLAAVFYTDGADIP
                     VTIEYDHNRFIHDSTQTVLIHGVSDAGRGTVTLFYPVYEIGTVHSPGLLEQYLHEIKV
                     DFWREKIDPSKPFKDQDLWKSGKYLGLSFDKRFKDSFAGLYRVDPDDRLTYLPFLQVP
                     ESREGMRYFKKWQNCPVYLN"
     gene            complement(29353..31425)
                     /locus_tag="PKN92_01870"
     CDS             complement(29353..31425)
                     /locus_tag="PKN92_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47570.1"
                     /translation="MGTADIDDIPGLKPFILKEKEYFDGKNIPADTVVQPLTTELELR
                     HLYYLYAVGWVWRLFGISQNALAFFTMLWYIAGVVAVYAIFLCFFKPFPSFVGAVLSL
                     TLPFHFHPLIGLREQGSVPFMLWILWGIVFVARKCKTRRSYFFVAFMSGAFIGIGQGF
                     RGEIPLYCPPLLVALYFFPNAISGFSVKIRLAALSVFLLSLSLCLIPLRQTMDSAHAH
                     TYFVGIAPDIEQTIPFGEAGYESLPSSDAAVYAMVSAFAKCRGDRESMVNEKSSEYAR
                     AQGDKNAPLLWDPSLFFNGEHYREQTFALMRQVIGSTPADLVARAWYSVFYLPGMPYQ
                     VLSSLEIKNFPEWLQKIVKFHYIFSLILCKSGMICSLFLLLCCSVISYKQAFFWFAML
                     AWFFGYPSLQYEFRNYYFLSFVPILILFLATSRIVQCAKLFDLKIKEKHFRVLFKEGV
                     AALKRPLIFTSFILLAVIAPVLALRSWQVHAISSIARELAGASLQEVETVSSLSGGAC
                     LVSPKETLPGLVNASSLPAGETAWQYVAVEVDTCGKDIPLTIRYNSRQIMYDFSQTVT
                     LFGIPDGKKGSLLFFFPVYEVDMNYGGTLMPSEILDIYPHVKDIVDSDVPIEEQQWWR
                     RGKFEGIAVPEEYRQHIRKMYIVSPDEGFCWLPIFQLPEDSRYLRCYKTGSLKKTFSR
                     WLKTLKST"
     gene            31808..33520
                     /locus_tag="PKN92_01875"
     CDS             31808..33520
                     /locus_tag="PKN92_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ47571.1"
                     /translation="MLYLGISEDMYDSGVTLMEDNTILFASNEERYTRRKNEGGFPHQ
                     ALAALLKETGINPADLDRICVCGHMTPPLPVRFFPFLQKAVYSVYRSKKDGLSKRLLD
                     FVLFHTPISHTKGDSPARKLTRPLLAPVVRRKLPKALRALPIGFTEHHTAHAAAAWFA
                     SGFDTCLCITADGMGDALSMTVSKADREGGVQRLWSAPAESSFGLFFEMLSQAFGFTP
                     CRDEGKITGLAAHGDPGKIDIPSPFSLEEGQLIYTGPRGLAGVKWIRKKLLTRYDRND
                     ISAWAQHLLEHFVLEIAAQWIEQTGLRKVAVSGGIFANVKLNQRLHELSQVEEFFVLP
                     NMGDGGLSLGAICEEAGLSPEKIQHVFLGDSFDDDATEKALGEAGLPYTKVEDPEETI
                     AELLADNKIVARFHGAMEWGPRALGNRSILARAVDPEITSRLNAMLSRSDFMPFAPAL
                     LLEDAALYCTHTEKAQHAAEFMTVCFRCTEKMAREHAPVVHVDGTARAQFVSQDSNPS
                     FHRILHSLKEKTGSSVVLNTSFNYHEEPITRTPQHAIGAFLRAGLDALMLGTFLVTNN
                     NEKE"
     gene            complement(33740..35443)
                     /locus_tag="PKN92_01880"
     CDS             complement(33740..35443)
                     /locus_tag="PKN92_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47572.1"
                     /translation="MQFMRIKSGILFLLLCGIPFLLLAGAEVTLRAIGYGQDYALFIP
                     CPDKESGTYTVNSHFYARNFYTELKKNIEETARWGTVLQKKKKEDVFRIFMFGGSVAM
                     GEDPDKAFNIPRFLEVMLQEAFPEVHFEICNLACFALGSAVMSDAAEQASCFSPDLYL
                     IYMGNNEYLGTMLSQWHRNGMPHKAWLVRCYYRAQQYRLVQLVQSFFPEKESPAPKDW
                     DDFYTNAFKLKAGTAGRERMYRNYEKNLQDMCRSARRGGADVLLSTVGTNIAALPPMG
                     SAHREGLAEEERVRWEQYFAAGCALLPQEPQQALDQFQKAMEIDPDPAKLHFHMGEAF
                     AALNRLAEAKSAFQEACDRDIYPVRADSHINAAIRQKAQEKDGATVWLADVEAAFAAA
                     SPIGIAGQEFCYDFVHQCVEGNYRIASVMFDAVADAVALRTGQMRQKEKLDYEHCCRS
                     LGMSPLLYVRCVNAILDDQEHLRELFPDYLPGRFTQLKKEAEAALPESAVDDALEAID
                     SALEIRGEDLQLRRLRCEILWDAGRAKEMHQEAQKILAHFGDTPLGAFTARSFILADG
                     Q"
     gene            35641..36267
                     /locus_tag="PKN92_01885"
     CDS             35641..36267
                     /locus_tag="PKN92_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47573.1"
                     /translation="MKPTRWLSLALAASLILLVSPGSYADRNPGKALRGIRNAVRSGA
                     RRPPTPFSRGILSQQMRGHNSNPLRSLSDLSRSHGDFRSPFHSGRRAYRQEDSMAKAY
                     RDVGIANAMVGLVGVLATLSQEPVYAPVCAPAPAPAAVPLAAPAAPSGHWERRPVAVH
                     PEHYEQYQVWIPAIVDARTGATTGGGYYETRSRLVPEQIEYQDVWVSY"
     gene            36342..37358
                     /gene="queG"
                     /locus_tag="PKN92_01890"
     CDS             36342..37358
                     /gene="queG"
                     /locus_tag="PKN92_01890"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNZ47574.1"
                     /translation="MDNSDITRQLKARALDLGFDDCGIADPSLESDDGLDAWLAQGKH
                     NGLQWLKKSRDKRQHIDLVLPGVRSVIVTASNYYHPGSKAQYGHARIARYAWRRDYHR
                     VILPRLKCLAAWIEHTLPGTKCYCSTDTGPVRERPWAARAGLGWIGRNSLLIHPIYGS
                     WMHLGIILCTAELVPDKPMPDHCGSCKRCLIFCPTDAIEPGRVIDTRKCIACQTIESR
                     SCETTLPSHNWVFGCDLCQEACPVNLQLQREDTTLEAPRQSFANLTLEELSSISEEEY
                     LTYFAGTPIMRAGHAAMQRFARHALTALPQTEGTLQHKKEGAISTETAPFFSKRLRID
                     LKRV"
     gene            complement(37480..38790)
                     /locus_tag="PKN92_01895"
     CDS             complement(37480..38790)
                     /locus_tag="PKN92_01895"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNZ47575.1"
                     /translation="MKTKTLMLALAVMAATFSTAYAQQGQGVELLRQIQDNFVQLHER
                     LRPAVVNIDVQGKAEAEAMTPFGPMEDFFRFFNVPEPQERQRPRQQMQRPRGTGSGFI
                     YDAQGYIITNNHVVEDAEDITVRLYNGHEFEAQIIGTDPETDLAVIKIEASEHLTPLP
                     LGDSDRVKVGEFAIAIGSPRGFEGSVSFGHISALGREGLVGLAMQGLTFQNLIQTDAA
                     INLGNSGGPLCNIDGEVVGINTAIVYGANSIGFAIPINTAKQTVPVLISQGKVTRGYL
                     GVSIDDANTYKDVDSLGLPDDRGAVVRQVQPDTPADKAGLKTYDVIRKVNGEEISNAS
                     ELVRKISAFSPGTTVTLEIWRDRQQISVDVNLTERTVVADRKAVEKSVLGMKVTEITP
                     EILQRLEMKSAIKGVIVSAVEPGSPAQEARLMEGDIIIEVGRKP"
     gene            complement(38901..39656)
                     /locus_tag="PKN92_01900"
     CDS             complement(38901..39656)
                     /locus_tag="PKN92_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47576.1"
                     /translation="MTTAILIVLLGFSQAAPDVAAALLAEGEHCFQQGNYKEAADLFS
                     RAHAEQGVCNPALFFNLGNAWQHEGSSGRAILYYEAALMLDPGFAPARQNLALALEKS
                     RRNLPLPDRRMLESPVLRYMPLKPLHGLYLTHALFLVAFVFALLLYWKSHRRYLVLAV
                     VSVLAALLLFGWTLAANQASRSLPQLAVACSEEVPVYFSTRESDTPRFVLYEGDRVFV
                     DRTEGEWLRVCVQGGEKGWAKKDLIGVVESVFH"
     gene            complement(39653..>41314)
                     /locus_tag="PKN92_01905"
     CDS             complement(39653..>41314)
                     /locus_tag="PKN92_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNZ47577.1"
                     /translation="YITISASGSEVKEPDLSPWHRLGFKTGAPATQSSTSIQSINGNT
                     TVVQSRSWRYPVAAGSPGTHTLPPLAVTVDGVQQMTRSLTLEISEKVSVSPEGRDGTP
                     AQEVAVDDLVFVRAIADKTTVYQGEPILLRLLIYVLDGRFVSLDTPRQLPLPETEGFF
                     SGPQWQNTYNGEYNSAAYRITEFNQVLFPAMPGDLQIASWTWQGVVRWQEGRFRPQGA
                     TRLFSTHPIPVTVLPLPEGAPAGFSGAVGKFRMQAYLSSASVAQGTPVRLTVSITGEG
                     NPQAIGMPLFEKPSWAHVSEPESEVKQTEGSLEVTKNYSYLITPLEAGEHVIPAVSFS
                     YFAPILKNYKTETSQELKLTVSATGETGRIVAVGGSSDTARQKIEINSSGELPLITDS
                     RLTALTVRPPAHILSMPALLSPVISAVMLVAAVLFLLRRKRLATDTRYARRIQARGKC
                     LALLQDAKEKGDKSAGIYQALSAFVADMLHINGSGLTSGEIQHLLQEARLNQESIDMT
                     VRVLRACERAAYGGGKGSEEELTVLMDSALHALDALHAAFLEEGK"
BASE COUNT        10367 a        11003 c        10380 g         9564 t
ORIGIN      
        1 gcagtgtaca tgggcgcagc ataattactg accacttttc ccagtgctcc cagacgaaag
       61 gcataataag agtctatttt cgcgcctctg gctgccgtca gcaacgccat ttcattctca
      121 atggcacgga gaggatcaga aagcagatca ggatagagtg cctcgatcat gggaggagac
      181 agagcggcgc ctgcctgaat atcactctgc aggcggttca acggcaaatt ctgttctctg
      241 gaaaccagct gcaatgccgt ggtaacgata acctgttgtg atctcggacc ccaggcaaaa
      301 gcaggtgtcg caagaagcag gcagatcagc agggcgttgc cgtaaaagaa attccgatat
      361 ttcatggaaa tgactccctg ttgtttacag atctgtttac tgagactggc gaatttgtgc
      421 aacaagcagc tcaatttcgt tggcatgcag tcccaccggg tcggactgag caagcttcag
      481 atactgatcc agctgcgcca gcccttcgtc cgtagcgttg cgtgcaataa ggagcaaccc
      541 cagctcaagg aaagcggggg catagtccga tgacatggac agagcacggc gatacgcctg
      601 ttccgcctgc atgctgtctc cctgcctggc aaaaatcata ccctgaattg ccgcctgccg
      661 tgccgtattc aagcccagct cagccgcttc acgcgccatg gccgccgcgc gatccagatt
      721 gccctgatcc gacaggatct gcgccagctg caacgccgcc tgcccggcat aattttttct
      781 ggcgctcaca agctggtcaa aagcggtaac ggcctcttct gtcagtttat tctcctgcag
      841 acaaagggcg tggagaaaaa cagcgcgctc attctctggc tgctgatgct tggcatcaga
      901 aaggagcggc agcgccttcc cgtattcacc tttgcccata taaagcgcac cgagctggag
      961 tttcacccag ccgtctacat gcggcgaaac agagggcagt gtactgagcg ttgcaatggc
     1021 ggaatcgctt tgaccttcct ccagacgcat tatccctgca gccgccagag cgtcagcatt
     1081 caaggtgctg cctgcctgct gctgcctctc gacaaactgc tccagtgccg gaagatcccg
     1141 atccagcgca taggcaagga caggcagaat atcggcgggt ttcgcaccgg atgccagcgc
     1201 cttatccata acgcctttct ggcgggcaaa atctccttct tttccgagtg tcagtgccag
     1261 cagcagctga acctctccgt caccggcaaa gcgtgttccg gcaaggtcgc tgagaaggct
     1321 ccgttgtttt tcccggtatt ctggttgttt gtcggcgatt tcttccgccg cgagcactgc
     1381 cagcagaagc gcatcccgcg ccgtactctc ccctttttgc gctacgagag caaaaagatc
     1441 atatgctttg tcatactcct gcatcttcca gtagaatttg gcaagcagaa actgcgcatt
     1501 ggttttgtca gggtttttct ggatatagtt tttaagggtg ctgacggcat gatcccaatc
     1561 ttctgaacgg gcatatttca atgcctcccc tacaggatcg cgcaagaggt aaagggtaac
     1621 aaataaggca attcctatgg cggcaaatac gagcagggcg gcactcaccc atttcgcagc
     1681 gacaacccat aagcttctgc ctgattcagg ggaagcctgt gctttaggag ccgccacttt
     1741 ttgccctgtc agtaaattgg ttccgcagga catacaaaga atatcgcccg gagagaccgg
     1801 ggtattgcac tgagggcaat gcgtcacttt caggggattg cttcctgctc ccgcaggcgc
     1861 ttttttcagc ggtcccccac attgaccgca gacagtggcg ccctgattga cggtgccacc
     1921 gcaataggga caatttttgc ttccagccac aacagtttcc ctttcatggt tgatcgggct
     1981 acactcagtt cctgtcaact atttactcaa gtgtaccata gaacacgtct gaggacagta
     2041 taatattccg gaaaagcgac tgcctttcgg ggcgctgcag gcgccacaga gattatacct
     2101 cagacaagtg atttgcagag atctttctgt tccgcttccg gtgcaatcca acaagatcac
     2161 tttttctttt tatgagcggc agatgatgcc gcaggcacat gatactgttt aggcacgccc
     2221 cagttatcat ccgcagcgct cggcttcggc ggcggcggcg gcacataggc tcttcttcgt
     2281 ctttttgtgg ggcggaaggg ccgaaacctg atcataagcc ctgctaccac gatcaaaaaa
     2341 aacagttcca tccaaggaat ccactccgga tcgtaatact cccagttcca gaattgtttc
     2401 cagggtgttg tccacgcctc gggatctgcc cgggtgccgt ctgcctgcaa cgcattcatt
     2461 gaagagttgt tcttgaacca gcagaagaga cgtaccagca catgcaccac cgtgtgcata
     2521 atcccggcat aaagtgtata ttccaagaca tagaggatcg ttggcaccga ataccatctt
     2581 gtgatataga tgagaagaag aagaaccggg acaccaaaaa tgtaataata actgtactcc
     2641 tgaaaatggg gaaagagcgg acggaaaaga tcaatataaa actccatacc tgaacctttt
     2701 atccctgagt tttctttggt taccaccaga atctataata ctccaagcgg tcatagttta
     2761 caacacagca ccgcatgcct ttgtcggggt tgccgctgac tccgattctt aacggtttgc
     2821 gcaggaaatg gttatgaaaa aaatactgtt caaaatcctt gtcgtcacca tcggtgctct
     2881 tattgtcctt attgcgggca cgctgctttg gttgaatccg ggacggggcc cggcagaggc
     2941 agtggacccc cacgccaaag gctgggctga agagatggaa ggcacacttg tgctgcatct
     3001 tgccggcacc ccctatgaaa tgggttatca gcgcggctat tttgccaggg aaaaagtgct
     3061 gctgactctg tcccttttcg aaaatctgct ggagctcgcc caggaagaaa tgggacttcc
     3121 gcgctttgcg tcgcatttga ttctggatct gacctatcag ctgtgctcgc catatattcc
     3181 ggatcgcttc aaacgcgagc tggaaggcat tgccgatgcg agcggatgcg atctgaaaat
     3241 gctccggcgt ggtcatgtga tttctgtcct gacggagcgg ggctgcagtg cctttgccgt
     3301 ctggggaaaa gcaaccaaag aaggaaaact gtttcacgga cgcaacttcg actggattct
     3361 gtccgccgga cttgaagaca cagctgtgct gtgcctttat gagcccgagg gctttcaccg
     3421 tttcgcctct gccgggtatg cgggtgtgat cggtgtactc agcggaatga atatggaagg
     3481 catctccatc agccagatcg gcgccattac caaggataaa tcactgcgcg gcctgcctct
     3541 ggagtttgtg ctgcgccgga ttcttgaaga aacatccaat ctggacgaag cagacgcact
     3601 catgcattcc gtcaagcata ctgtcggatt caattatgtg gttgccgacg gagatgccgg
     3661 tgatgcccgc gcctttgaga cgactgccca tcatatcgcc accttcggcc cgggtgatcc
     3721 gaaagaaaca gtggaatatg ctatccccat tgaagacgcc gtcttccgct ccgacgaggc
     3781 aatggaccct cttatccgca gtcttcagga atgcgccaat gcgcccggcc tgccctacgg
     3841 ctccaactcc tacgatcacc gctacctggg aatcgcaaaa agagtacagg aacgttacgg
     3901 cacaatagac gcggactatg cgcttgaaat cctgaaagcg acggcaatgg ataatgcaaa
     3961 cctgcacagc gttctcgcca acagcagcga ccgtgaatta tggattgccc atgcgaaaaa
     4021 aggacagcag gcatccctgc agccctatat tcattatgat ctgaaacaat tatttttacg
     4081 gccggaagaa aggaatcctg ttgcgcctcc tccggaaaca cactcggaaa cagcggcgga
     4141 aacagacact ccaaccggaa acacagaagc ggcaactgca gaagcctctt cataacaggc
     4201 gttttatgta gaaactgcaa gaagttcctg cgaaaagaag caggttcaac cagccggact
     4261 tcagacggca caaaaaggga acacaatgct gaaaggatta ggtgatctgg gcaaaatggg
     4321 cggaatcatc aaacaggcgc tcgagatgaa aggcaagatc gaagaattaa aagagaaaat
     4381 gggggatatg gagattccct atgccatcgg cggcgatatg gtcaccgtag tcatgacagg
     4441 caaactggaa gtgaaatcgc tgactatttc tcaggaactg cttgagcaga acgaccgtga
     4501 aatgctggag agtatggtgc aggctgccat gagcggcgcc attgcaaaag cccaggatgc
     4561 ggtcaaagag aaaatgacag aattaaccgg cggtatcgat attccgggat tatgactatg
     4621 agaatcgttt ttttcggcac agcagaaatt gcagtgccct gtctggcatc tttatgcacg
     4681 gcacatgaga ttgcggctgt cgtcactcag ccggatcgcc ctaaaggtcg cagtaaaaaa
     4741 cctgaaccgc cggaagtgaa agtttgggct cttgaacacg ggctcgaagt tgttcagcct
     4801 gaaaaactga acgacgggaa cttcgagagg tggctccggg aaaaaaaacc ggagctgtgt
     4861 gctctggcgg cttatggcag gctgctgaaa cagcccattc ttgacgtgcc tctgcggggg
     4921 tggctcaaca tgcatccgag cctccttccc cgatggcgcg gtccctcacc ggtacagacg
     4981 gcgctgctcg ctggtgatga cgtgaccggt gtcaccatta tgcgcattac gctggaaatg
     5041 gatgcgggca atattgtgct gcaggaaaag ctgcctgtcg gcagaacaga aacggcaggc
     5101 gaactgtctc gccgagccgc cgatctcggc ggacggctca tggcacaggc tgttgcccaa
     5161 atggaaacgg gcaaactgac ggaatatccg caggaacacg atcaggcaac tttcagcaga
     5221 atgcttcaga aagaagacgg catcatccgg tgggatagag aagctgcttc catacacaac
     5281 cagattcgcg cctgcaaccc ctggccggtt gcccactgtc tgttccggga tcgcatgctg
     5341 cggcttctgc gaagcgatta cagcgaaaaa tcacacagtg cgctgccggg cacggttatt
     5401 caggcggaca ggcaaggtat cgttgttgca accggtaaag gatgcctgac cataacagaa
     5461 ttgcaatggg cgggcaaaaa cccgctggca gtcgacgcct ttttgcgtgg caccccggtg
     5521 cggcccggcg actgctttca ggaggttacc tgatggcagt ggatccggtt cgtgatgctg
     5581 cagtgtctgt gctgcttcgt gtctttcaga cagaagcgca caacgatgta tccatagaca
     5641 gaaccctccg gcgcacaaat ctgtcgccac ggggcgcacg ctttatgacc catcttgtgt
     5701 acggtgtagt acgccaccgt cttttgtgcg accatgtact gcagcccctt tgcgatcagc
     5761 cgctggatca actgccgctg tccgtattaa tcgttctgcg gatgggtgtt tttcagtcat
     5821 ttttctgcag caatgtcacc cggcccgcac tggtgcacac aggagtggat ctggcgaaaa
     5881 agcgcggtca ttccggactc gccaaggtag cgaatgccgt gctgcgccgc ctcccctctt
     5941 cacttgatga aatcacactg cccgaccgga cgacacagct gatggagcac ctgcgcatac
     6001 gctattccat gccgcgatgg cttgtgcgcc tgtggtgcga actgtatgga gaggacggtg
     6061 ctgaagccta ttgtgccgca tgtgattccg ctgcgctccc gtcagtacgc atcaacacgc
     6121 ttctcgcaca gacagacgat gtgctgcagc agatgcagaa agcaggcatc gccgctgaaa
     6181 gaatcacgga agcactggat gcgctgctgc tgcacgacgg caccaacccg caggcaaccc
     6241 gctggttccg tcaggggcaa ctgatgcagc aggatccggc atccattctg gcaggtgctc
     6301 ttgtcgaccc gaagccgggc gaaaagattc tggacatgtg tgcggcaccg ggcggaaaaa
     6361 cgctgcacct ctgcgcactc agccgggatg cggcgcccat ctttgcgctg gagcgttatg
     6421 ccggacgggt ggcacgaatt gtagaaaacc gcgaacgtct gggagggaca caggtactcc
     6481 ccctgtgcgg tgacggactt cagccgcctt ttcaggatgg cactttcgac cgcgttcttg
     6541 tggatgcgcc ctgttcgggt ctgggcactt tgcgccgcca tcctgaaatc aaatggcgca
     6601 tccagccgga aacaccgtca cttttagcgg caacacagaa agccatgttg cgaaaggcac
     6661 tgcaactttg taagaatggt ggacttgtcg tttattccgt ttgtaccccc accccgcagg
     6721 aaactacaga agtgaccgca gctattcttg ccgaaggggg ttgtatgccc gaagacgggc
     6781 cggagcaatt tgattcgtgg aaaacagccc tgggacaata ccaaacaagc ccgaaaaacg
     6841 gagcgtggga cgctttttac ttgatgcgtt ttcgaaaaca atcctgagtt cggtcttttt
     6901 tattatccgc tttttcgtct ggacgctgca gtggtccttt gcagttgtgc tgtttctgct
     6961 ggtcatggcg ggtacaggct actatgtata caatgtcacc ctgaaagggc gtgcccatgt
     7021 ggtcgtgccc aatatcgttg accgacctat ctctgaagct gcctttctgc tttctgaaaa
     7081 acatctggaa atcggcaagc aggtgcaggc aacccacccg agcatcccaa aatattatgt
     7141 gattgcccag cgccccgcag cgggtcgtgt cgcacgggaa ggcaggcggg tgaatgttat
     7201 cgtcagtatg ggcgaagatt acatgcgtgc gccggatctc accgggaaga acctcgaaga
     7261 ggcacgccgg gaaattcttg cctcccgctt ccgcgtgggc agtctcgccc gtattcccga
     7321 agagcagccc cgggacaccg tactatcgca ggatcccccc gccggagcgc ggctgcccga
     7381 acagggcgag atccatctgc tggtaagtgc gggaagcggg cgacccagtg cgcttatgcc
     7441 cgatctgcgt ggccttgcca tttccgatat cgcaagagcg atggaaggct ttgatgtagt
     7501 gcttgttgcc aatgcggtgg atattcccgg agccgttccg gacattgttc tggcacagtc
     7561 gccccctccc gacaccttga tctatcagaa tcaggtggtc acctatgatt acctgcgttc
     7621 ctccggcgaa aaagcgccta cacagcaata tgaggacaca gtacgccatc agatgttcta
     7681 tgactggtac agccgtgatg tccgggtgga tgtcgtcgac cgtctgggta atcgtcagac
     7741 gatttggaca aaaaagccgc tatttgatga agaagcgctg agaacctatg tcaccggcac
     7801 tgtgattcgg gttcctgtga cctacattga tgaagtttct gtggaagtct atattgacaa
     7861 tgaacttgag gcttcttata cactgaaaga aggtcgccct ccggtgaaaa tccggtgatg
     7921 gcccctgtgt tcaaaagtta agagcaggaa gcgacgggga tgcagcaaga gattctccgc
     7981 gtactctgaa tgaaactaac tggaatcaga aaacgatgtc agccaacaca attaaaatat
     8041 ccccttctct cctctcctgt gatttcagcc gtctgggcga agaaattcag gcaattaccg
     8101 atgccggtgc cgacatgatt cactgcgaca ttatggacgg ccattttgtg cccaatatca
     8161 cttttggccc gcccgttctg gcaagtttgc gggcacattg ccgcctgcct ctggatgtgc
     8221 atctgatgat agaagagcct gaaaattatg tacaggcttt cgtggaagcg ggtgcggaca
     8281 ttcttaccat tcaggtggaa tcgacccggc acccgcaccg gctgatccgg cgcattcagg
     8341 atctgggctg caaagcggga atcgtactga acccgggcac cccggaagac agcatcaaat
     8401 atctggcgga tatggtggat atggtgctgg ttatgtcggt gaacccgggc ttcggcggac
     8461 aatcttttat ccgggaaatg ctgccgaaaa tcagccgtat ccgtgatatg atcggcgact
     8521 gcgacctgca ggtagacggg ggaattgatg aacatgtggc ggcggacgtc attgctgccg
     8581 gtgcgaatgt gcttgtggcg ggctcctata tcttcaatca tcattcctat gcgaaggcaa
     8641 tccggctgct gaagactgcc caccacacct gatcccgggc tcagcttccc atttttttca
     8701 gaatggcgga caaccgctgc tcgcactgga tggcggcacg cacggtatga taattgatct
     8761 tccaggcatg tcccatataa tcccagagca ggttgttgtg ctcatcaaag agcgggtacc
     8821 actcacctac ctcgtgattg atcacacgat ccatcacaaa gcggtgcaca ttttcgtagg
     8881 cattccagta tttctcttca ccgaaaagca gaaatgcatc cagcatgccg accagcgtct
     8941 ccgcctgctg ccagaattcc ttattccttt cccgggcagg cccgtcgccg gggccctcgc
     9001 aaaagacacc tccccgctcc cagtcaatac catagctgtg gcaatggtcg tacagctttc
     9061 gcatacgctc tttaaagggg tcaacgtcaa gacccagaat acgcaatgaa tgcatgagca
     9121 gccaggcaaa ctccacattg tgcccgaagc tggtattgtt catgggacgt cccacatcat
     9181 cctccacatc ccgatccgag ccccatacat ttttgaaaag gatagcgcgg agcggcgtcc
     9241 agtcaaaaga aaactgggca atgccggtac cgtacacggg atgaatcatg cgctcgaaaa
     9301 tgagttcgat gacccgtacc gtatcccttt tgtggcggtg gttcctcgtt gcctcataca
     9361 ggttggtgaa cgcttccatg aggtgcatat gcacgtccag cgacttgcgg tctccgccat
     9421 aaacgccggg tttcttcggc gaccagtctt cctcgaaaaa ctcaccgaag ccgccatact
     9481 gaagatcggc ggagcgggtt ttaataatgt cataagcgtc cacggcatgc tgcagcacag
     9541 cggaatcctg agctgccatg gcatattcgc tcagcgcata aacggcaaag ctgtggccgt
     9601 acatgatttt ggcacgatca agaggggtac catcgcgctc acttgtccaa taccagccac
     9661 cattctcacg atctttgaaa tgcttcagca ggaaatcaac gccctgtttt gccagaggaa
     9721 gaaatgttcc gttatccagc ccggcacgag aaatggagga aaaggtatag atcatgcgtg
     9781 tctgacagac cagcgttttt acgccttctc ccgtaggatc gccgttttta tccagatacg
     9841 ttaaaaagcc gccgtattct ttatccacgc cccgggttga ccagaacgga agcagttctt
     9901 ctgtaagatg atgcttcatt tcgctgtggg ctttttctac acgttctttc aaagacataa
     9961 caaaattcct ttcttcctga tcggtattca agactgttct ttttcagaag ggagctgctt
    10021 ctccgttccg gaagccttgt cctgaaacag cgtcattaac ttttcccaat atgcaaatac
    10081 tatccaaagc atgaaaggca gggtcaggaa aaattgcacc agtgccgtcg gcacttcgct
    10141 gatcagcatg agtttatcct gaaaacgcat ggcaatgatg ataaagaaag catgaaaaaa
    10201 gcaaagaatg ccacagccgt agagcaggat aagcccgcgc cgtcgccagg tcaatccggc
    10261 tgtagcaaga accagcgcaa tatacggagg gacattggtg gcaagcagtg ctattttcat
    10321 gcggcgctca tgaccggaaa cagaaaaaac aagactgctt tccgtattga gtttgccctg
    10381 cacgtcgatg aatccggagt cgataggaac acccagagca tatttcagcg gcaatcccgt
    10441 tatctgcagc aatgcctgtc cgtaatacgg aaggcactcc caccacacca caacaaaaaa
    10501 aacgacaaac accataaatt tcagtgcaaa gaatggcaga gcaaaacgtt gtcccttagt
    10561 ggtcatgagc gcaccctttt caccacgtac tcgacccaga ccagccaaac ggcaacgaca
    10621 aaaataatgt agactgcctg ccagacatac tcgtgggcga gattgaacca gacgcggctc
    10681 tggtctaccg ccccgataat ggcgagcacg gtaagccgga aaatattcac gccatacatg
    10741 acgggaattc ccgccgccag accgatgaca cgcttacgcc aacaggttgg aaaggcaagt
    10801 atggcagcga caaaaatcgc catgatttct atggcaccac attcgggcac taaaataaag
    10861 gggaaaacaa gagaaagatc ttcttttccc tcctgctgct cctgccgaag cgcctgcaca
    10921 tcgtttttca ggtgctcgag ctccgactgc agcgcaggcg catccgaatc aggctgggct
    10981 tccaactgat taattttgcc ggaaaggcta ttgatcctgg acgagatacc ttccctaaac
    11041 acgaaataaa cgcgcgggcc gtttacacgc cgggcatcct gctttctggt tgtgacagca
    11101 cgccatgacc agcgttccca ggcggaaagc ggttcgtcgg aaagcagagc cgtttcctcg
    11161 gaagaaaggg attctttgcc ttttttccac gcctgcaggg cggcgcgggt tcccttaata
    11221 tccaggcggg aataatgcag cggctccact tctccgtgcc cgatacggtc aagaacccag
    11281 gcggtatgac gggcagcctg aaagaggtac cagtcattga tgcgggtagg gatcagatac
    11341 cggtaggaag tgagcagcac aagggtcgta ataacaaaaa cagcaacaaa aagaaaaatc
    11401 cgtttccggt tttctctgga ttgtcgcttt ttttctgaaa gtgtctcact tgattcctgg
    11461 ttctgtgtca tccccgggca ggctccttga aaggtggaat atgaatagag aaagtatgcc
    11521 acaaaaaagg agaacacaac aaaaatatgt gatccaacat tgcagcaagg gctcgggtca
    11581 ttcagaatgc ctgccggagt gagaggttta ttcctgtgcc tgaatcatat agtataaaca
    11641 gccggatcga ccgatttttg ataaggaagc ggcgcaatga gaaaaaatga tatatgggct
    11701 gtactggaca tagaaacgtc ccgttcaata ccgcccgctg ccatcgtgca gatttcctgt
    11761 cagcgcatgc gcggctggcg ggctgacggt gcgccgttcc gcatgctgct gaatcaaagg
    11821 gtggtgatcg atccggatgc ggaagcaaag cacggatttt cccgggagta tctggaagaa
    11881 tacggttttg cgcccgggta tgcttaccga aaactcaggg actatgcgga agactgccca
    11941 acggttatga gcgagctcgc attgaactgg aagcactttc tcgaaccgga catggcaaga
    12001 acaggctttt cagagctgcc gcctccgcgc ttttgcctga cggctcttgc ggaacggcta
    12061 atccccgaaa cgggagaaca caaggctgaa acgctactcg cactttttca aaccgaaaca
    12121 gacctggaaa aaacgcagct ttcccctgac acaccgctat gggtaattct gctccgggct
    12181 gtattgcgga aaaggttgaa tgcccttggg atcacaggat ttgaacagat agaaactttt
    12241 tccaaatcct ttcccgtaga ggacagcaaa aaagcagtta gggaagcact ggaagcgccg
    12301 gaacaatggt atgtgctgac ttcagggcag ctaccgaaag gtccttatcg ccttcgccat
    12361 attgtcgcca tgtcaaaagg cgacaactgg cttgtcgccc gtaaaggagc agaacagtgg
    12421 acaccactcc ggcatttgcc tgaatttcgt caggcacagg aagccaacga gtacattcag
    12481 gcaatatcag gaaagggaga caagccagcc acgaaaccga ttcttctggg aagccgggaa
    12541 gcggtgtcat ccgcccctct tttcccgtcg ctgaaagcag cgctcaagaa aagcaccgca
    12601 tcggaggata tcactgactc cgctcaagat gctgtaaaag ttttagtgga tatacttagg
    12661 gaaatcgatt ctctgaatga ggcaggagaa gcggcaatgc agtctctgcg ccttcaactg
    12721 gcacaatgtc ccgatccgca cctctttccc cttccctatc tatccaaagt actggagtca
    12781 tttccacatg acaggaaact ccaaccggag gaaaggcaag aactgctggc ttctataagg
    12841 gaaattctgt ctctcctgcc atgaaaaaaa tgacggaagc gctgcttttc gcctttatat
    12901 ccggttttct ccaaatttaa gatgcacgga atacggatct tctgttacac tttagggaga
    12961 tggtcggaac tttttgagtc aaaaaaggat agtacttgta aagacattca gtacttatcc
    13021 ggataagtca gtgaatacat gaataatcaa tccaacacgc acaaagaaga gcgtgcatct
    13081 gcctcacacc tgcggatcgt cagtcaaaat ggttatccga cggactggtc ggcgctggac
    13141 gatgacagcc tgatggcaaa acatcagcag ggagaagagc gcgcctttga agagttggtc
    13201 aacagatacc agcgaagtat attttcttat gtctaccgga tgctgcaaaa cagacatatt
    13261 gcggaagaaa tcacgcagga agtttttctt gcccttgtcc gcaacagcga ccgatacaaa
    13321 gcgcagggac gcctgtcgtc ctaccttttt gccattgctt ccaacaaagt atacaaagag
    13381 tgggaacagc ggcgacggcg cccgcacttt ttatccctgt ctttctggag cagtgacaac
    13441 gggttctccg acggtggctt gacaccgctg gacaaactgg atgacaaaca ggcccgcgtc
    13501 gataaggcat gtgaacacat ggaaatttca gaggcgatca acgatgcgct gcgacacctt
    13561 cccgcccatt accgggaagc attcatcctg cggcgtttcc aggatctgcc ctatgacgaa
    13621 atagcagaaa tacttgactg ccctgtgggc acagtcaagt cccgggtagc acgtgcggaa
    13681 aaggcgctgc gcccgtatct ggaaaagttc agagactata tagagtaagt gcggagtttg
    13741 tgaaatgtcg tcagattccc ctatcctgcg ccatccctcc tcaatccaat tgatggagta
    13801 tgcggaatca ttggtggata acgacgctcc cgtctgtgcg ctgattgcat cacatgtgtc
    13861 ccgctgcccg gagtgtgcgg cggaagttcg cgccatccgg cataccctca aagtatcgac
    13921 gctctcacag ataccggaag ctgaaaacag ccttgtgcag gcgattcttc ttcaggcgcg
    13981 caaagaggcg gcggcagaca gcgcaaaaac agagcgaaac cgctggtttc ctgttttgcg
    14041 cggtgccgcc tgctttgccg gaatcattct tctgggaatg ctttcttttt cagcggcatt
    14101 gcatgaaggc tccaaagccc cctccatcgc acttcaagct gaagcacgcc ccatccctgc
    14161 tccggaaaat ggctggaacc ccgtcagacc cttccacggc tcggtggtgc aggcgctcgc
    14221 cgcctccgta gcgggttccg gggatggatt atccagaagc gcagacgcag gctatcggcg
    14281 tatggtagat tttcttgatc aggatctgga tgcggcgaca gcggcgttgc agcgtaatcc
    14341 gggctgcgtc cgggcaacac aaattctcga tgccgaggca aaaaaaagac tggagggact
    14401 gcgcaccctc tttatagacc gcaccctctg agaggcacct tcttttgagc agtaaaaagg
    14461 cagcatagtc atggggcgaa tacttctatc actgttgctt cctggactgc tcctgaccgc
    14521 ttctccttcc tctgcgcagc tgattcagca tttacagtcc ggcattgaag aagtcgcccg
    14581 ggggatggac tatgtgggac gccgcgccgg aacgctattc agcggcacag aagggaaaaa
    14641 cacagccaat gacacgctgc cttccgtgca gcgggaggtg gaggaacact accctgccac
    14701 acccgcttcc actctttttc tcgccaacga attcggtgat atccgcattc gctgctggga
    14761 cgaacgcctg attcagatta aggcggttat tcaggtgagt gcacccacag aagatctggc
    14821 ggaacaggtc tcatcgctga tcgagctgcg tgttgaacag gaaggggact actgggaatg
    14881 ccgcacctct ctcccggaac tgagaagcgg agatgccgtg gcgatgagtg ttcattacga
    14941 ggccgctatt cccaaagatg ccacgctgac aatcaacaac tatttcggag atgtctatgt
    15001 gaatgacctt gggggagcgc tcaccgccga gatccagtac ggcgccctgc acttgtccca
    15061 catccagggg ctggtgcgcg cccgcattca gggggagttt cctgttcgcg ccaattatct
    15121 gaatcagggc ggcaccttta tcatgacagg aacggacgcg gaattttcaa attttcaggg
    15181 taatctcgac attacacaat ttcgcggttc cgtgacagta cgccatccgg gtcccgagtc
    15241 tacggttaac ctgatcagcg aaaacgggcg gacagaaatc ctgctgcctc ctgatgcgaa
    15301 tcctgatttg actgccaccg tcgcacacgg gcgctttgaa tccgagctgg atgtaacccg
    15361 ggtcgtgcgg ggaaagcagc tgctggcaag gcacctcaat gccgaagcaa aacaacgctt
    15421 catggtgaat gcctctttca gtgatgtact tgtcgggatt gaaggcggca tgtctcagac
    15481 agtttcacgg atgagtgctg atcagaaagc ctttaccgac accaccacta ctctgattcc
    15541 ttttgagtcg ggaaatacga tggttattga tgccatgccc ggcaacattc atatcgaagg
    15601 tgtggacgaa gatcagcttt ccgttgaaat gacccgcgtt gcctggacac cctccgccgc
    15661 tgccgccatg gatgcgctgg atgctctgga aatcaagact gtgaaaaccc ccggcttcat
    15721 ccatcttacg acacgtgcca cacaggacat gaccgccttc tcgtgccagt cctatcgcgc
    15781 cgatctgcgc atcaaatgcc cgcgcacatt accggtgacc ctgaatgcgg agaagggagc
    15841 aaccgttctg cagggtgtgg gcgcggactg tgttgtgaat caggaatcag gcgaagtaca
    15901 gtttgaacat atgcggggca tgcttactgt atcaggaaac acgacctcag tcacagccaa
    15961 atcctgtatc ggccccctca tcataaacgt tgcatccggc gatatcacag ccaaagagat
    16021 ttttgacagc atctcaatta ataacgggga agggcgtgtt cttgtggata caccggggaa
    16081 ggacaccgag gttcggcagc gcaaaggtga cgtgcatttt ctaagtttag cgccccttgc
    16141 cggcaaaatg gatatcttta ctgaagccgg tgatatcaat gtcttcatcg ctcctgagtc
    16201 aagcgcttcc attacagtga aaacacaggg cggacagata cgaagtgcct tgcccctgac
    16261 aggcagtatc aacctggaaa gccaggaatt tttcggacgc attaacacgg gcgagcacac
    16321 tgtgcttctt gaaacggtgc agggcagtat ctatttaaat taacggactg tacctctgat
    16381 tgaacgtgaa ggagtctggg ctgatggcgg gcaggcgaat agccatgcgg atgtttctct
    16441 tcggatgcat gggactcgtt ttcgagatct tctacatagc actgcatgat gccgtgtttg
    16501 gcggcagctg gaatctgcgg ggacactcat cactttggat gataccggtc tatgcgcttc
    16561 ccggtcttct caccggccct gtatctcatc ggctgaaaaa ccggcatatc ccgctgccgt
    16621 tccgtgcttt catttatatg ctgatgattt ttgccgtgga atacgtaagc ggtgttttat
    16681 tcacagccgc agggctggat atctggaact acagcaacct gcgctggaac cttcacgggc
    16741 atatcgccct gtcctacgtt ccggtctggt attttctggc attgtggctg gaatatcttt
    16801 atcgcctgat tgatggctgt gcctgtgttc tggttaaatc tcataaaact actcagtgat
    16861 cagatcttta caaaataaat aattgtgcaa aagccgtggt ttgtagtatt atattgatca
    16921 ggatagtaac ggaactgtag cgggagcctg acatggaaga catgcacttg ttactggata
    16981 agctgggcga caaagaagcc gcctgggtag aacgccgtga cgcagctgaa tcaatcggca
    17041 aagaacttca gcagattctg ggccgcctta cgaatcatct ccacgacgaa gacatggacg
    17101 tcaagatggc ggtagagcgc agtctggatc ctgtgcatct gctgctgcgc actcgagaca
    17161 agggtaacaa tgaattcagt ttgagggatc ttgccttttt ctgtgagaag acaggtgtgc
    17221 gctctgtcaa accatacaag gcaggtttcg ttattcgggt acgccttcct catggcaatc
    17281 agcagagtgt atacctcctg ccacatcagc tgcgggacgg acgccagacc gttcgggttc
    17341 ttgccatctg ctgtgaagca aacagccata ctgttcggaa ggctcttcgc accaattcca
    17401 aactggcgca cagcgccttt tccatgaccc gttctaaaaa cagtgtctac atttcccttc
    17461 tcagcaacgt tttccgtcag gaagcaacac cggacaggat taaaagagcc gtgaaagagg
    17521 ttgcttatta tgcggactgg atggcgcatc tcaatgcacc gtctgaaaaa gatgaagaaa
    17581 gcgaattcga tcaggactaa cggttcctgc tgaaagaagg taaatgatga agccccacga
    17641 aacccctgat gtggtggcag gtgttggtgc atgctgcgct ggtgaaagca tcggaccgga
    17701 agatgccggg tctttggatt ttcacgaaaa agatctttcc gggcagtgct tttccggtgc
    17761 ctctctcgta ggcgccaatt ttcgcggtgc acttctgcgc ggcacccgtt ttgacggcgc
    17821 agatctcagc aatgctgatt ttaccggcgc ggatcttgaa ctggctgtgc tgggcggcgc
    17881 cgtactccga aacgcttctt ttcactccgc caatctcgag cgcgccaacc tggtcggggt
    17941 agatgcggca ggggctgatt tttccggcgc acgcatgtat ttttcaagac caggaaacgg
    18001 caacttcacc ggcgcctcct ttcagggcgc acaacttgac cgtgccattt tcagaagggc
    18061 gacacttgta aattgtgatc ttagaggtgc caccggtgtg cctaattttg aaaatgcacg
    18121 gcttgacggt gtcataaaat gatttttgcc cacttccgta cagagattaa cgagtccaac
    18181 cagttcgtca tcgcctgcag tcaggaaaaa caggcgctgc tcattgacgc acctgttctg
    18241 gacaaacgga ttcccgcctt ttttgaagca cacggtctct cgctcgccgg tatttttatc
    18301 acccacaccc attatgatca tgttgaggga ctggcagaca tcctccagtg gaggcaggcc
    18361 ccggtttatg caggaaaaag cagcatagcc ggaatactgg ctgaaaagaa aaagcaggat
    18421 gaccctcttg atctgggctg cttaaaaggc agattcatcg cattgccggg gcacactgcc
    18481 tgctccctcg gacttgttat tggagagcgg gtttttaccg gtgatgctct tttcgccgga
    18541 agcatcggcg gcaccccgga tgacattaca aagcagcagg agattcaggc agttcagcag
    18601 cagatattta cgctgcctca tcactaccag gtgcatacgg gacacggtcc ctcctccacc
    18661 gtatggattg agaaaacctt caatcccttc tttgtgtagc atcagtcgac aggggtaaaa
    18721 agtgccagcg ctctgcaaat ctgcacagta gcccgcgact tgttgagcac atagaagtga
    18781 atccccggcg caccctgctt gatcagctct tccgcctgcc gtgccgcata gtacacgccc
    18841 acggcatact gcccctcatg gtcatcttca taaacctcaa gccgtttcac cagtttggat
    18901 gtcaaatgcg caccgcacat gttgacaatg cgccgggtct gagtaagatt ggtgacgggc
    18961 agcaccccgg ggataatagg caccgtaatg cctgcatcac tgcagcggtc tctgaaccga
    19021 aaataatccg cattatcaaa aaagatctgg gtcacaatga aatcagcgcc ggcatcaacc
    19081 ttccgcttca aattttctat atcggcggcg gtagatggcg cctcgggatg cgtttccggg
    19141 tatccggcaa cactgatccc gaaagcagga aactccgatc ggatgagaga aacaagttca
    19201 ttcgcatatc ggagcccgtc ggcaggaggc gtatattccg gatcctcctg gggaagatcg
    19261 ccccggagcg caacaatatg ggtaatgccg atctcctgag cctggcgtaa aaaagcccgc
    19321 agttcgtcaa cgctgcttcc tacacaggtc aaatgcgatg ccagaggaat ctgcgggtag
    19381 gtatcgtgaa tttctctcag gacaccaagc gtccgctccc gggaactgcc ccctgccccg
    19441 taggtgcagg taaaatatgc cggcttgcac gccgccagtt ctttcagatg cagaaaaaga
    19501 ttttccagac cttcctgtgt tttcggggga aaaagttcaa aggagatcac gggctgcccc
    19561 gtatcataaa acgttctgaa ctttgtcatg aaagactcct gtttacaaaa cacaacatca
    19621 ttgcggaaaa catgccttgc tctcagcttt ccgctgttgt ttcacccggg gcgtactctt
    19681 ttcggatacc gtcaatcata cgggaatacg cctgcagcga aatttccttg tctaccttgc
    19741 attcgattat acatgactct ccggcatcct gcacagcaga ccagcatgtt ttaaatgcgc
    19801 ccacagtttc gggagcatac catggcagtc cgaacatggc tgcagcaccg ctgaattctc
    19861 gccggtgagg atgaacaaaa catggtacga gcacttcggg aaatgcagca atgggaagat
    19921 gatgaaaaat gccgccacct ccattgttca cgatcacaag tataaccggg gtcggcgaag
    19981 ccgccacaag tgcaagtgaa ttaagatcat ggagcgcagt catgtcacca atacaggcta
    20041 ccgtcggcct ttcgcatccg aaggcgatcc cggcggcagt ggcgatattg ccgtctatgc
    20101 cgctcacacc ccgattggcg tgaatgagaa aagcacagca gggctgatcc agcatatcca
    20161 tataacggat cggcatgctg ttaccggaga aaaaagcggt gccttcggtg atccgctcat
    20221 gcagcagaag aaaaagggat agctcagtaa gcggtgccgt gtctgtcaag tcattcagga
    20281 tctccacggc acgagaaaag agcccttccg tcgggagtgt ttctgcctca gcccgaagtc
    20341 ggtctatttc aggcatattc agtatggaac gacatacaaa agcgatgctg ccctgcagat
    20401 gacgggtcac caggtgcgca gggtctcttc tgtcggaccg gggatggaca tgaacataga
    20461 gttccggggg actggcctgt aaaaactcgt agagtgcttt ggaaacgaag gtgtcgccca
    20521 aatgcaggat tgcatccgcc tgaaaaaagt gcccaaggca tcctgccttc gccagaagat
    20581 catatcctga cagaaaagat aatcccgttt catttcttct gcagtttgaa aggatgtccg
    20641 gcagcaccct ccagccgagt cgctttgcaa gcctgcatgc catttcctgg tcttcctgcc
    20701 tgtgcatcgc accgagcaga agagccttat tctttttgcc cgcaaaagat tccagtaccg
    20761 ccctaatttc ctcaggatca ggctttttat cgctatcggc atgatcgcac cagggcactg
    20821 taccggcatg ccactctttc agttcatcca gataatcttc ctgcccggaa gcaggaacag
    20881 gaacaggggc gagtggctcc cggaagggac agttaatgtg aacaggaccg ggcgcttgag
    20941 cgaaacagtg cgacaacgcc tgatcaatac gactcagaaa cagccccgca gggatgcccg
    21001 catcgggaca gggaagagaa agagatgctc gaacatggct gccgaaaagt cctgcctgct
    21061 ccatggtctg atttgcgccg cattggtgca tttcaggcgg acgatcggcg ctcagtatca
    21121 caagcggaag ccccgaaagg ttcgcttcgg cgactgcagg aaaacaattg gcaacagctg
    21181 tgcctgatgt gcagatcact gcagcaggca cccccgcccg ggcagcaccc aaagcaaaaa
    21241 aggcagcgcc tctttcgtca tagtgaaccc gggtttttac cgatggatta cgccaggcag
    21301 ccactgcaag aggagcgctt ctcgacccgg gggcaatgac aaagcacttt atgcccaggc
    21361 gcgtgacctc ctctatcaga agtgtcgccc aaagcatgtt catgtttccg ttatgattca
    21421 tgcatgcgcc cttcgatcac acctgcgaac tgatgtattt ttgcgtccag ctccctccac
    21481 tcctcttccg ctacggagtc gggaacaatg cctgcaccgg cgtaaaggca cagacggttt
    21541 cccaaaacag cagcactccg gatcccgaca aaaaagtcag cactgtcccg ggcaatccag
    21601 cctacgggtg cggcaaaaat accccgctca aaaggctcat gctcacgcag atactggagc
    21661 gcttcttttt taggataacc tcctacggca ggcgtgggat gcatctgccg caacagaaaa
    21721 gaatcagaga cgccctgctc cagacggaca cgcacaggcg tgtacagatg ctgacaatgt
    21781 gccagttttt ttattgccgg tttccccgga tattggagct caacagcgaa attatccagc
    21841 gttgtcagca gcgactctac aacaatggca tgttcctgct gctctttttt gctgcagaca
    21901 agttcttttt cgagccgtgc gtcctctgcc ggatccagtc ctctcgggcg tgttcccgcc
    21961 aaagcctcgg tctgcatatg cacgcctctt ctgtggtaca accgttcagg ggatgcaccc
    22021 agaaaagcac ggcaggatgc cggctgaaag caaaacaatg tcgtatcagg ggctgtatct
    22081 gccaaaaggc acatcagggc tatcggatcg acggctgcag atgcgtgaaa agaactttcc
    22141 cgtgccagca ccacttttgt caatggtccg tctgcaaatg cgtgcagtgc gtcttcgatt
    22201 tgttttttcc atccggaaaa atccggcaga tcactgcgcc cgctaaaaac aggcagctcc
    22261 ggaagcactg cctgttttgc gctgatcatt ctttctaaaa gggcaatcag tttcctgcgc
    22321 cctccgtcat ccttcaccgc agccgcaaac accgcttttt cattctcacg gcaaaattcc
    22381 agaagaggaa taacgaagcg atgcgcctga aaatcccgcc agagtccgtg gcgcgcggga
    22441 gaggaatgga aacgaaagcc gccatagtat cggatccctt cgctgccgtt cttgagacgg
    22501 tcctgcatca gcgcaaacac ttgctcaata tcacggccgc cctgcgcatc gagaacgtct
    22561 gcttccccaa cacctgccat ttcaaccgtg cccttccggt cacgccaata atagcggggc
    22621 acacctgtca gagaaccgag ccaggtcaga gcatcaagat caggtgcttc aatctgcagc
    22681 ctcactaaag ctccagacga agagaggggc gtttctttga ctagcgacag aaaacggcgc
    22741 aagcagctgc atgcggtttc ctgctgttta agtcggtcag tttccaataa ttcagacata
    22801 gattctctgg tgtgtgttaa agaaaaggaa cggcggcatc gctgaaattg acgcagccta
    22861 taggataatc aaaaatggca gcggtgccca aataattttt tgaaagactg tctgcaggca
    22921 gttttttttc agcactttga aaccccggca gaggataaag gccggttcag gttgcagttg
    22981 cggggataca tcggtaaaat gacattgctt gtcttcagat tctttttcgc aattccaatg
    23041 gaacaccgtt tccccttttc actgaaagac gcatggctca tacattctgc gcaacgctgc
    23101 gacaccacaa gcatattcct gttctgatgt ttctttttct gacaggtctg ctgctgcgtg
    23161 cctcctggct tatggagatt cagagaactc ctgatttcca aattcccctc ctttatcagg
    23221 tagacatggg tttcatcgac aacagcgctc tggattatgc acaggctctg cgtgcattct
    23281 ttcacctgcc cgacatagag ctgaaatttg aacgtcagat tgtgtggagg caccatgcag
    23341 gcgatccctt gctgcgaccg cccggataca ctttttttct ctcgctgtta tattttcttt
    23401 tcggggacaa tcagcttctg gtacgcatag ttcagatgag tcttggcatg gcgggcggct
    23461 tgctcggttt ccttgccggg agaaaactgg cgggcgtccg ggcgggcgta attgccgcag
    23521 gcgccctgtg gctttactgg cctcttattc tttatgaatc tgtgctgcac gagccggtac
    23581 tggttgtttt tgtttccctt ctttttttga acagcgcgct acagtggctt aaaacaacag
    23641 ggctgaaaca agctgcgaaa accggatttt tgtgcggcat ctttactgtt tgcgcttcac
    23701 aaattatcct cttccttccg gtcatgcttc tctggttcct gtggttcggc tgcaaacgtt
    23761 tcaggaatct gcgtcgaagt ttatcgcacg ccggcttggc gggactcttc ttcttcctcc
    23821 ctctgcttcc ggtctcctgc ctgaattatt atgagtcagg catgtttgtg ctcaacagcc
    23881 acggtcatgg gattacactt tacatcggca accagcccgg tgctgagggg cttctgatgc
    23941 cgggaaagcc actggctgaa gagtttctgg ggcttcagga ttcaggctta agagcagatg
    24001 agatagagac cttattcagg actggactgc ctggtctcat ttttctcaaa aggcggcaat
    24061 ccggacgata ctaagtgctc ccggagattt tatcaaaagg tccttccggc gcactttcct
    24121 attctgggct ccattcgaaa tatcccagaa cgttcttgaa tattgtgacc gcctgttttc
    24181 ttcatcgtta tctaagattc cgggtaattt tggaatagta ctcccctgtt taatgctcgg
    24241 tgtattttat tttctgcttc ttctgctgcg ccgattccgg aaagagcgca ccgcggaaga
    24301 ctggcaagag tatagaacat tcgcaggatt gtgcctgatt tgcctcctga ttgtggtctg
    24361 gtaccttccg attactgtgc tgtgggtctc tgctcatttt cgaatacctc ttctgccttc
    24421 cttctttttg cttgcggctt tcggcatgac cagattcctt gctgacatca aaggagccca
    24481 gtggcgccgg gccgcattga atggtttagg cgcatctgtt tttatcctgc ccgccttcct
    24541 ttttcactgg aattacgatc aggatattca aacctggctt taccaccatg taaaggtgta
    24601 tgaaaaccag ggcaattcag aagctgcagc tgctcttgct ttatttgctt ctgaaaaagc
    24661 acccgaccat gcctttgtgc agaaaacagc cggtgatctt cttcttgatt caggacgtaa
    24721 agaagaggca atgatccatt acgcaaaagc acgcaaatgt acgcagggcg ttatagatca
    24781 gacagaattg ctggcgcaaa tgggtattat tcagcgggac tccggaaaca acgacgctgc
    24841 ggaactgctt tttcaggaag tgctgcagca caatcctgaa catagcacgg cattgcattt
    24901 tctgggtaat attgcctatg aaaaaggggc atatgaaaca gcagaaacac tgctgaaaaa
    24961 agcggtggcg ctggagacaa cacagggaaa cacttttttc ctgctgggct tgacacagcg
    25021 ggcacaggga aaaaccgaag aggcggaacg ggcatttcga cagggacttt tttttgaccc
    25081 ggacaatctc tggctgcagc ttgaacttgc cgatgttctg actctcctgg gtcacaaaga
    25141 ggaggcctgt gcgttgtatc agaaagtact tgaaaacaat ccgggaaatg aacgggcgca
    25201 aaatgcactt tccgcacctt agtgcgaaga tcagaaaaaa taacatcggg gtggatcatg
    25261 aatcagaagg taaaaacctc gaccacagac aattctttct gttttttatg cgctgagtac
    25321 tattgtgcta gaatcgcttg ttagattccg ttctcaacac gcaacgtagc ggagaagttt
    25381 atcgtccgat cttaaatttg aagaagccct ctcctgcctt ccgacggctg cgggcattcc
    25441 ctttgtgttt ttccaaaccg cattgtgaaa taattacttg ccgccaaatc gtttatttga
    25501 agaaacatgc gtctgatctg cccccacatg gaaagatttt tatgcgtatc cttttcgtgc
    25561 tgaacattcc ctatgccata gaaccccatg gggtcatgct gctctcgtcc atctgcaaaa
    25621 acgcaggtca tgaagtgcag ctcatactgc ttaaaaaaga cagtttgata aaacgggttc
    25681 aggaatttaa ccctcatgta gttgcctaca gcgttatcgg cagtgaaatt gatattttcg
    25741 cagccgccga tgaaaaactc cgccgttact gtgaggaaag cggcacccgg atatttcgga
    25801 tcatgggagg tccgcacccc accttttcac ctgggatact ggatcagatg tcactcgatg
    25861 ccatctgtca gggcgatggc gaaaaagcgc tgcctgcttt actggaatgt cttgaaaaga
    25921 acgagccgct tgatgacata ccgaatatag cgcttaccag tgccggagcc aggctgagag
    25981 aaaaactgag cagcgaggaa ctggacgccc agccctttgc tgaccgtgac ttgtattatg
    26041 cggcagtacc ctatatcccc agaacaggac tcaggtcctt tgttgcatcc agaggctgtc
    26101 cttttcactg cactttctgt tataaccacg tctacaacaa aatgtttgaa ggctgcggac
    26161 cgatcctgcg gcggcgttcc gtcaacagcc ttctcactga aatagaacag gttattgccc
    26221 agtacccgcc ggtgcggttt attcgctttt ttgatgatac tttttccctg accgttgatg
    26281 actggctgcg ggaatttgcc gaagaatatc ccaaacgaat cggtatcccc ttctactgtc
    26341 tcatgcggcc gaacaccttt tctgaagaag cggctgtgct tctgaaaaaa gctggatgct
    26401 attccctttc catggcagtg gaagccggtt ccgagcccat acgcaatacc attctgaaac
    26461 gcggtctttc cgataaagat atgcggaccg cttttgaaag agcacgcagg ctgggcatta
    26521 acacctatgg gaataccatg gtcgccgtgc cgggcacaac gattgaagat gattttcact
    26581 ccatggaatt cacgcgcagc ctgaagctga ccgtgcccac cttttcggtc tgcagtccct
    26641 caagaggcac tgaactcgcc gactttgcca tggaacaggg actcatgcct gccgatgcgg
    26701 atatgattac ccgcttttcc gctgaaagcc ccctcaactc ctatacggaa aaggaaaaga
    26761 agatacaggc acgcatcgcc tgtctgggca ctctgtactg tacagtaccc ggttttctga
    26821 aaccgctggc aaaactgatg attacaagcc ccttccccat cgggcttgcc cgcaaaatag
    26881 gtttctcctt cgccattttc cggatcgcaa cccgcctgtt cccccatgcc atccccaaaa
    26941 atccgatcac gatcgtacgc gttgccatag acggcatccg ctatttctgg taaacacgtg
    27001 aagaaaacag gctagtttaa atagacaggg cagttttgcc attttttaaa atagcgcatc
    27061 ccttcccggc tctctgggac ctgcaggaaa ggtagatacg tcagcctgtc atccggatca
    27121 accctgtaaa gacctgcaaa agagtcttta aaccgtttat caaaagagag ccccagatac
    27181 ttcccgcttt tccacagatc ctgatcctta aagggcttgg agggatctat cttctcacgc
    27241 cagaaatcca ctttgatttc gtgaaggtat tgctccagca gcccgggcga atgaacagtt
    27301 ccgatttcat aaacgggata aaaaagtgtg acagtgcccc tgcctgcatc cgatacccca
    27361 tggatcagca ctgtctgagt ggaatcatga ataaacctgt tgtgatcata ttcaatagta
    27421 acgggaatgt ctgctccatc cgtataaaaa acggcagcca ggtattccca ggaggcttct
    27481 ccgggagcat ggaagtcttc atcggggaga ccgggtaacg gttctgacgg tcgcacaagc
    27541 atgctgcttt catttccctg aacatcttct gctgtaacag aaaccggata ccgcttcaga
    27601 ctttctgcaa tatccagcaa agaatgcacc ttctgtttct gccagagacg cagcggtacc
    27661 agaggcacta ctacggcaag acatagggtc aggctgaaca gcagtatttt tttcaacatt
    27721 tcaccgctgc caaatataca gcgggcttca gaacatggga aatgattatg aatctgatcc
    27781 ggatgctgct ttttccggag tgcctgccag cccgatacag cacaattatg tacccactcg
    27841 aggcctgcca gcaaaacaag aaacggcaca aaagacaaat acgccatatg acgtgtatca
    27901 aaacacacgc tgacatagcc tgtgcaccat aaaatcaaaa aggcgagtcc gtaggctatt
    27961 ttcaaattcc gcgcgctcag cacaagtaaa gaaagaagaa ccagacacaa tcctccatat
    28021 cgaatgccat gtgctgccag agtgtgcccg gtaaatattt tctgaatcag caatggaaaa
    28081 gaagcacgaa cctgcttcgt catcatacag acttccaggg gcatggaata aaaggcactt
    28141 gtggaaaccc aggcacggta aacaatatcg gcgggaaaat gttttaaata atctaaaaac
    28201 agaatctgcc catattcgcc atattttgca ctggtgtagt acaagttggg attaatcatt
    28261 cgtggagcat ctttttcgcg ctgctcctgc ctgtattccg ccgtataaac atttgccatg
    28321 gaggaggtat ctccccgacg cctcgccagt acatttgcct gtgcatacag cccggcatct
    28381 gtccagaaga gggcatcata ggaggcacat ccgaaagaca tcttttcttc tatatcaggg
    28441 gacactcccg taaaaaaact atgcgtgaat gcctggttcc cttcaagagc cgctccatat
    28501 cgggcaggcg cagaaagcag ataaaaaggc acaacaaata acaagcctgc ggctaatcgc
    28561 cagaaaaaac tgtgattact tttgaacttc gtgccgacca gaaaaagaag agccaggggc
    28621 ggaacaatca ttgctacatc ctgacggatt cccatcgcca ttcccagcac aatccctagg
    28681 cctaaagaaa aaaacaggta ctttcggagg cctgccggtt ttaaaaacaa aaacaatgcc
    28741 atccataaag ccagcagtac aaagggtgtc tttgcaaaat ccctgaggtt attgcttgta
    28801 tataaaaaaa caggcgatga cacgaccaga aacactcctg ccagactgta gccacggcgc
    28861 agtcccagac gaaaaatacc gaacagggca acaccggcaa gagcacttaa aaaggcggag
    28921 cacaaaagca tggagtgcac tgatatgccg aagaaccgcc agaaccagcc aacaaggtac
    28981 aaagtgtaga ggtgcgtcag ttcaaagggg ctcgatacag gaagggttct tgtctctgac
    29041 ggtatatttt gaatatcaaa atgatcagtg ttcctaagca ggaaatcttc cagaccgggg
    29101 atgtcactcg catcagccgt tccaagtcca tgtcctgccg caaaaaaaac agagggaaaa
    29161 aaaagattca tcgccatcgg ggccgcatga tcaagcgggt cttccagaga aactccataa
    29221 acatagcaga tgaaaaaaga aagaatgcat gcaaccagta ccaaaagtgc ttcaaagcaa
    29281 tacttctttt tcaacgtttg acgctttcat ccggaataaa tttgcagcag ttcactatcg
    29341 gcctctgttt ttctaagtac tttttaaggt tttcagccat cgactgaaag tttttttcaa
    29401 agagcctgtt ttataacagc ggaggtagcg gctatcctcg ggaagctgaa aaatgggaag
    29461 ccagcaaaag ccttcgtcag gcgacacaat atacattttt cggatatgct gccggtactc
    29521 ttccggtacg gctatgcctt caaatttacc gcgacgccac cattgctgct cctcgatcgg
    29581 aacatcagag tccactatgt ctttcacatg gggatatata tccagaattt cgctcggcat
    29641 caacgttccg ccgtaattca tatccacttc gtaaacagga aagaaaaaga ggagcgagcc
    29701 ttttttaccg tccggaattc cgaaaagagt tactgtttgt gaaaaatcat acatgatctg
    29761 acgcgagtta tagcgaatag tcagaggaat gtctttcccg cacgtatcca cttccaccgc
    29821 aacatactgc caggcggtct ccccggcggg caatgatgat gcgtttacaa gaccgggaag
    29881 cgtttcttta ggagaaacga ggcacgcccc cccgctcagc gatgaaaccg tttccacttc
    29941 ctgcagggaa gcccccgcga gttctcttgc aatactgctt atggcatgca cctgccaact
    30001 tctgagcgca agaacaggag caataacagc caacaggatg aacgatgtga aaatcagagg
    30061 acgtttcagt gctgccacgc cctctttaaa taaaaccctg aaatgttttt ctttaatttt
    30121 taaatcaaac agcttcgcac actgcacaat tctgctggtt gccagaaaaa gtataagaat
    30181 agggacaaat gaaagaaagt aataatttct aaactcatat tgaaggctgg gatatccgaa
    30241 aaaccaggca agcatggcaa accagaaaaa ggcctgttta taacttataa cactgcaaca
    30301 caaaagcaaa aataacgagc agatcatgcc actcttacat agaatcagac tgaatatgta
    30361 atggaatttt acaattttct gaagccattc gggaaagttt tttatttcca gactggacag
    30421 tacctgatag ggcattccgg gcagataaaa aacactgtac caggctcggg ccacaagatc
    30481 cgcaggagta ctgccgatta cttgccgcat caacgcaaat gtttgttcgc gataatgttc
    30541 tccgttaaaa aatagagaag gatcccatag aagcggggca tttttatccc cttgggcacg
    30601 cgcgtattct gacgacttct cattcaccat ggattcacgg tcgccacgac acttcgcaaa
    30661 agcactgacc atggcataaa cagcggcatc agaagaagga aggctttcat aacctgcttc
    30721 tccgaaaggt atagtctgct caatatccgg ggcaatgccg acaaaatacg tgtgtgcatg
    30781 ggcactatcc atcgtttgcc tcagagggat caaacaaaga ctaagcgaaa gtaaaaacac
    30841 acttaaagca gcaagacgta ttttaacgga aaagcccgat attgcattag ggaaaaaata
    30901 cagcgccacc agaagaggcg gacaataaag cggtatttct cctctgaagc cctgaccgat
    30961 gccaataaaa gcgcctgaca taaaggcaac aaaaaaataa gatctgcgcg ttttgcattt
    31021 tcgtgcgacg aaaacaatcc cccataaaat ccacaacatg aaaggcacac taccctgctc
    31081 acgcaaccca atcagcggat gaaaatgaaa aggcaaggtc aaagacaaaa cagcgcccac
    31141 aaaagacggg aaaggcttga aaaaacacaa aaatattgca tacacagcca ctacgccggc
    31201 gatataccac aacatcgtaa agaaagccag tgcgttctgt gatattccga agagacgcca
    31261 gacccagcca acggcataga gataataaag atggcgcaat tccaactcgg ttgtcagagg
    31321 ttgaacgact gtgtcagcgg gaatattctt cccatcaaaa tactcttttt ctttcagaat
    31381 aaaaggcttc agaccgggaa tatcgtctat atcagccgtt cccattccca tgcccgcagc
    31441 aaaaaaaact gccgggacta atagatttgt ctggagaaat accgggtgct cctgcgggat
    31501 tctatagaga aaaacgaaag aaaaagaaaa agccaaaacg agaaggctag acaaccaaaa
    31561 caattcaagc actttttttg agccacgctt ctgctcgtta agcaatccca taaataaatc
    31621 ctgataggac cgaggggaca cctgaagaat gtatctgttg ttcccttcgg acggcggtca
    31681 aggcagtaaa ggagcacttc taaatggtat actataaata ctttataaat ctaccgccat
    31741 gcttttaaat ggtgccatac aggcacaatc aatttatagg caaagcctca ccatccagga
    31801 agcccacatg ctgtatcttg gcatcagtga agacatgtac gattccggtg taacgcttat
    31861 ggaggataac accattttgt tcgcctccaa cgaagagcgg tatacgcgcc ggaaaaatga
    31921 aggaggcttc cctcatcagg cccttgcggc actgctgaag gaaaccggaa ttaatcctgc
    31981 agatttagat cgcatttgcg tttgcggtca tatgacccct cctctgcccg ttcgtttttt
    32041 tccttttttg caaaaagcag tctattccgt ttatcggtcc aaaaaagacg gtctttccaa
    32101 gcggcttctg gattttgttc ttttccatac ccctatttca cacaccaagg gcgattctcc
    32161 tgcccgcaaa cttacacgcc cgcttcttgc ccctgttgta cgccgcaaac tgccgaaagc
    32221 acttcgagcc ctgcccatcg gatttacaga gcaccataca gcacatgccg ctgcggcgtg
    32281 gttcgcatcc ggatttgata cctgtctatg catcactgcg gacggcatgg gcgatgcatt
    32341 gtccatgact gtttcaaaag ccgacaggga aggcggcgtt caacggctct ggagcgcgcc
    32401 agctgaaagt tctttcgggc tttttttcga aatgctgtct caggccttcg gatttacacc
    32461 ctgccgggat gaaggaaaaa taacaggcct cgccgcccat ggcgacccgg gaaaaatcga
    32521 tattccgtct cccttttctc tggaagaggg tcagctgatc tacaccggcc cccggggttt
    32581 ggccggtgtt aaatggattc gtaaaaaact cctgacccgg tatgaccgca acgatatcag
    32641 cgcctgggca cagcacctcc tggaacactt tgttctcgaa atcgcggcac agtggattga
    32701 gcagacggga ttacgaaaag tcgctgtatc gggcggcatt ttcgcaaatg ttaagctgaa
    32761 ccagagatta cacgagctga gccaggtaga agaatttttt gtactgccca acatgggaga
    32821 tggcggcctg tctttgggtg ctatctgcga agaggcagga ctttccccgg aaaaaattca
    32881 acatgttttt ctgggggact cttttgatga cgacgcgacg gaaaaagcgt tgggagaagc
    32941 cgggctcccc tatacaaaag ttgaagaccc cgaagaaaca atagcggaac ttctggcgga
    33001 taacaagata gttgcccgtt ttcatggcgc catggaatgg gggcctcgtg cgctcggcaa
    33061 ccgctccatt ctagcccggg cagttgatcc ggaaattact tcgcgcctca acgccatgct
    33121 gtccagaagt gactttatgc ctttcgcccc tgcactgctt ctggaagatg ctgcgttata
    33181 ctgcacacat acggaaaaag cacagcatgc ggcagagttt atgactgtct gcttccgatg
    33241 caccgaaaaa atggcaagag agcacgctcc cgttgttcat gtggacggaa ccgcccgtgc
    33301 acagtttgtc tcacaggaca gcaatccgtc attccatcga attcttcact ccttaaaaga
    33361 aaaaacaggg agcagtgtcg tactgaatac cagtttcaac taccatgaag aacctatcac
    33421 acgaacgcca caacatgcca taggagcctt tctgcgcgcc ggactggatg cactgatgct
    33481 tggcactttt ctggttacga acaacaatga gaaagaataa tcagaaaatt agcggtataa
    33541 ttcgtgcccc ctggtcgtgt aaattcgtat gaacacacag gactttattt cctgatccag
    33601 tttgcagcca aaacgatctg tacactatct ttactaatgc tatgagcatt ctttctgccc
    33661 ataaactctg attatcatga ataactctct tttccagagg tattaaagct ccgccttata
    33721 ccggcaaagg agttctattt cattgcccgt ctgccagaat aaaagatcgg gcagtaaatg
    33781 cgccaagtgg cgtatccccg aaatgcgcga ggattttctg cgcttcctga tgcatctcct
    33841 tcgctcttcc ggcatcccaa agaatttcac agcgaagccg gcgcagctgt aaatcctcgc
    33901 cccgaatttc cagagcgctg tcaatcgcct ccagcgcatc atcaaccgca ctttcgggca
    33961 atgctgcctc tgcctccttt tttaactgcg taaaacgccc gggaagataa tccggaaaca
    34021 gttctctcaa atgctcctga tcatccagta tggcattgac acatcttaca tacaataaag
    34081 ggctcattcc cagcgatcgg caacagtgct cgtaatcaag tttttctttt tgccgcatct
    34141 gcccggtgcg aagtgcaaca gcatcagcaa cggcatcaaa catgacggaa gcaatccgat
    34201 aatttccctc aacgcattga tgcacaaagt catagcagaa ttcctgtccg gcgatgccta
    34261 tgggtgaagc tgctgcaaaa gcagcctcca catctgccag ccaaacagtc gcgccatctt
    34321 tctcctgcgc tttttgcctt atggccgcat taatatgcga gtctgcacga acaggataaa
    34381 tatccctgtc acaggcctcc tgaaaagcac tttttgcctc tgccagtctg ttcagtgcgg
    34441 caaatgcctc gcccatatgg aagtgaagtt ttgccggatc cggatctatt tccatcgctt
    34501 tctgaaattg atcaagggcc tgctggggct cctgcgggag caatgcacat ccggcggcaa
    34561 agtactgctc ccaacggact ctctcttctt cggctaaacc ttcacgatgc gccgacccca
    34621 tgggcggcaa ggcagcaata ttggtaccga ccgtagacaa gaggacatct gcaccgccac
    34681 gccgggcact cctgcacata tcctgaagat ttttttcata attccgatac atgcgctcac
    34741 gcccggctgt accggctttt aatttaaatg cgttcgtgta aaaatcatcc cagtctttag
    34801 gtgcagggct ttctttttcc ggaaaaaaag actgcacaag ctgaacaagc cggtactgct
    34861 gcgcccgata atagcagcga accagccacg ccttatgtgg cattccgttt cgatgccact
    34921 ggctcagcat cgtgcccaga tattcgttat tacccatgta aataagatag aggtcgggag
    34981 aaaaacagga tgcctgctcc gctgcatcac tcatcacagc tgaaccgagc gcaaagcacg
    35041 ccaggttgca gatttcgaag tgcacttcag gaaatgcttc ctgcaacatg acctccagaa
    35101 agcggggaat attaaaggct ttgtccggat cttcacccat agcaacggag ccgccaaaca
    35161 taaaaatccg aaagacatct tcctttttct ttttttggag cactgttccc cagcgcgctg
    35221 tttcttcaat attttttttc aattcagtat aaaaattgcg cgcataaaag tgactgttga
    35281 ccgtgtatgt cccgctctct ttatccgggc agggaataaa gagtgcgtaa tcctgcccgt
    35341 agccgatggc gcgaagggta acctcggctc ctgcgagcaa cagaaaagga attccgcaca
    35401 gaaggaggaa cagaataccg gattttattc tcataaattg catgaaaaga attcttccag
    35461 gaaaataagc accattacgg gcgacagtgc tttgcatggt aacacagcgc ttttatcctg
    35521 ctcaattgcc tttccttccc tgcagtgcaa atgaatagag ccgggctgac atcgtcttta
    35581 ttcgtataga atgcaggttt tcaggtaatg acgcgttcac aacaaataag gagcacaccc
    35641 atgaaaccaa cacgctggct ctctcttgcg ctggctgcaa gtcttatcct tctggtatcg
    35701 ccgggatcat atgccgaccg aaatcccggg aaagcactga gaggcattcg caatgcggtg
    35761 cgttccggtg cccggcgtcc gcccactcct ttctcccgtg gaattttgtc ccagcaaatg
    35821 cgtggtcaca acagcaaccc gctcaggagc ctttcagacc tgagcagatc gcatggtgat
    35881 tttcgttcac ccttccattc aggaagaaga gcataccgcc aggaagattc catggcaaag
    35941 gcttaccgcg acgtaggcat cgccaatgcc atggtcggtc tggtcggcgt gcttgcgacg
    36001 ctgtcacagg agccggttta tgcgcccgtc tgtgcccccg cacccgctcc cgcagctgtt
    36061 ccactggcag cgcctgcagc tccgagtgga cattgggaac ggcgaccggt agcggtacat
    36121 cctgaacact atgagcagta tcaggtctgg attccggcga ttgttgatgc ccgcactggc
    36181 gcaacgacgg gcggcggcta ttacgaaacc cgttcacggc tggtcccgga acagatcgaa
    36241 tatcaggatg tgtgggtatc ttactgactt cttttttaag gaagcaaaaa acgtaatagc
    36301 aaaattcgca acagtcggac atttcgtcat attatagtcc gatggataac agcgatataa
    36361 ccaggcagct caaagcccgg gcattggatc tgggctttga tgattgcgga attgccgatc
    36421 cgtcgctaga gtcggatgac ggtctggatg cctggcttgc gcaggggaag cacaacggac
    36481 ttcagtggct taaaaaaagc agagataaac gccagcacat cgatctggtt cttcccggtg
    36541 tccggtcagt gatagtcacg gcgagcaact attaccatcc cggctcaaaa gcgcaatacg
    36601 gtcatgcacg cattgcccgt tatgcctggc gccgggatta tcaccgggtg attctgccca
    36661 ggcttaaatg tcttgccgcc tggatcgagc acacacttcc gggcacgaaa tgctactgca
    36721 gcacggatac aggtcctgta cgtgaacgtc cctgggcagc ccgggcaggt ctgggctgga
    36781 tcggaaggaa cagcctcctt atccacccga tctacggctc ctggatgcat ctgggcatta
    36841 ttctgtgcac tgccgaactg gtgccggaca agcctatgcc cgaccattgc ggcagctgca
    36901 agcgctgtct gattttttgt cccaccgatg ccattgagcc cggacgggtt atcgacacaa
    36961 gaaaatgtat tgcctgtcag actattgagt ccaggagctg cgaaaccacc cttccttctc
    37021 ataattgggt tttcggctgt gatctgtgtc aggaagcgtg tccggtaaat ctgcagcttc
    37081 agcgtgaaga cactacgctg gaagcgccgc gtcagtcttt tgcgaatctg acactggagg
    37141 aactctcttc aatttcagaa gaggaatatc tcacctactt tgcgggtact cctatcatgc
    37201 gtgcgggaca tgccgctatg cagcgttttg cccggcacgc cctgactgct ctgccgcaaa
    37261 cagagggtac acttcagcat aaaaaggagg gcgcgatctc cacggaaacc gcgccttttt
    37321 tttcaaaaag actcagaatc gatctgaagc gtgtctagtg tttcggcacc cggatgatgg
    37381 tgatatccgg atcacggtca ccgcgaacaa agcgtaccag cagccctttt ccgggctcgg
    37441 cgtgcttatc caccagtttg aagaattcgc ttgcggagct tacggcttcc tgcccacttc
    37501 aataatgatg tcgccttcca tgaggcgtgc ttcctgggca gggctaccgg gctccaccgc
    37561 gctgacgatc acacctttaa tggcggattt catttccaga cgctgcaata tttcgggtgt
    37621 aatctctgtc actttcatac ccagcacgga cttttccacc gccttacgat cagcgacaac
    37681 ggttctctct gtcagattca cgtccacaga aatctgctgg cggtcgcgcc agatttccag
    37741 agtaacggta gtaccgggcg aaaaagcgga gattttgcga accagttcgc tggcattcga
    37801 tatctcttca ccgttcactt tgcggatcac gtcataggtt ttcaggccgg ctttgtcggc
    37861 gggcgtatcg ggctgtacct gccgcacaac agcaccccgg tcatcaggaa gcccaagact
    37921 gtccacatct ttgtatgtat tggcatcatc aatggatacg ccaagatacc cgcgcgtcac
    37981 tttgccctga gaaatcagca caggcaccgt ctgctttgcg gtattgatag gaatggcaaa
    38041 tccgatcgag ttggcaccgt agacgatagc ggtgttgata ccgaccactt cgccgtcaat
    38101 attgcagagg ggaccgccgc tgtttcccag attaatggcg gcatccgtct gaatgagatt
    38161 ctggaaagtc agtccctgca tggcaagacc tacaagccct tctctgccca gggcgctgat
    38221 gtgcccgaag gacacagagc cttcaaagcc gcggggactt ccgatggcaa tggcgaactc
    38281 acccactttc acccggtcgg aatcgcccaa aggcaggggg gtcagatgct cgctcgcctc
    38341 gatttttatg actgccagat ctgtttccgg atcagtgccg attatctgcg cttcgaattc
    38401 atgaccgtta tacaggcgga ccgtaatgtc ttccgcatct tccacgacat ggttattggt
    38461 aataatatag ccctgtgcat cataaatgaa gccggagccg gtacctctcg gccgctgcat
    38521 ctgctggcgc gggcgttgac gttcctgcgg ttcgggcaca ttgaaaaagc ggaagaaatc
    38581 ttccatgggt ccgaagggag tcattgcctc cgcctccgct ttgccctgta cgtcaatatt
    38641 gacaactgcc gggcgcagcc gttcgtgaag ctggacaaaa ttgtcctgga tctggcgcag
    38701 caattccacg ccttgacctt gctgtgcata agcggtactg aaagttgccg ccatcactgc
    38761 taaagcaagc atcagtgttt tcgttttcat actcaggttc tccttaattt tctgggaggt
    38821 gatcgatccg cactccatta tgcgtatcgg catctcctct tctttccaaa gacacaaagc
    38881 ctttacaggc tattttgctc tcaatgaaac acgctttcaa ccacccctat caggtctttt
    38941 ttcgcccagc ctttctcccc gccctgcaca caaacacgca gccactcccc ttccgtgcga
    39001 tccacaaaca cacgatctcc ttcatataac acgaaacgcg gagtatcgga ttccctggta
    39061 ctgaagtaga caggcacttc ttcgctgcag gctactgcca gctgcggcag tgaccggctc
    39121 gcctgattcg cggccagcgt ccagccgaaa agaaggagcg ccgccagcac ggaaacaacc
    39181 gcaagaacaa gatatctccg atggcttttc caatacagca gaagagcgaa aacgaaagcc
    39241 acaagaaaga gtgcgtgcgt gaggtacagc ccgtgcagcg gcttcaaagg catataccgc
    39301 agcacgggac tttccagcat gcgtctgtcc ggcaggggca gatttcttct ggatttttca
    39361 agggcaagag ccagattctg acgggcaggg gcaaaccccg gatccagcat cagcgccgct
    39421 tcataataaa gaatggcgcg cccggaagag ccttcatgct gccaggcatt gccgagatta
    39481 aaaaagagtg ccgggttgca tactccttgc tcggcatggg cacggctaaa aagatcggct
    39541 gcctctttat agtttccctg ctgaaaacaa tgctctcctt ccgcgagcaa agctgccgct
    39601 acatcgggag ccgcctgcga aaaacccagc agaacaataa gaatcgccgt tgtcatttcc
    39661 cttcctccag aaacgccgca tgaagcgcat caagagcatg cagtgcggaa tccataagca
    39721 ccgtaagctc ttcttcagaa cctttcccgc cgccataggc agcgcgctca caggcacgga
    39781 gaacacggac agtcatgtct atgctctcct gattcaaccg ggcttcctgc agaagatgtt
    39841 gaatttcccc ggaagtgaga cccgagccgt ttatatgaag catgtctgcg acaaaagcgg
    39901 acagcgcctg ataaatacct gcgcttttat cgcccttttc ctttgcatcc tgcagcaggg
    39961 caaggcattt gccacgggcc tggatgcgac gggcatatct ggtatccgta gcaaggcgtt
    40021 ttctgcgcag aagaaacagc acagctgcaa caagcataac agcgctgatt acgggactca
    40081 gcaaagcagg cattgaaaga atatgtgccg gagggcggac tgtaagcgca gtcagtctgc
    40141 tgtcggtaat gaggggcaat tcgcccgaac tgttgatttc tattttctgt cttgctgtat
    40201 cggaagaacc gccgactgcc acgatgcgtc ccgtttcgcc tgtggcgctg acagtcagtt
    40261 ttaattcctg agaagtctct gttttgtaat ttttcagaat aggcgcgaaa taactgaagc
    40321 tgacagccgg gatgacatgc tcgcctgctt ccagcggtgt gataaggtaa ctgtagtttt
    40381 tagtaacttc caggctgcct tcggtctgct tcacttcaga ttcaggctct gagacatgag
    40441 cccacgaagg tttttcaaaa agaggcatgc ctatagcctg aggatttccc tcaccggtta
    40501 tgctcacagt caggcgcaca ggcgtgccct gtgccactga agcactggac aaataagcct
    40561 gcatacggaa cttgcccacc gccccgctga aaccggcagg cgcaccctcg ggcagcggca
    40621 gcacggtcac aggaatcgga tgagtactga aaagtcgcgt ggcaccctgc ggacggaacc
    40681 gcccttcctg ccagcgcacg actccctgcc aggtccagga agcaatttgg agatccccgg
    40741 gcatcgccgg aaaaagcacc tgattaaatt cggtgatccg gtaagcggcg ctgttgtatt
    40801 ccccgttgta cgtattctgc cactgcggcc cggagaaaaa accctctgtt tcgggaaggg
    40861 gcagctgacg cggtgtatca aggctgacaa aacgtccgtc cagcacataa ataagcagcc
    40921 gaagcagtat gggttctccc tgatacaccg ttgtcttatc ggcaatggca cggacaaaaa
    40981 caagatcatc tacggcgact tcctgcgcag gggttccgtc tctgccttca ggggatacag
    41041 aaaccttttc gctgatttca agcgtaagag accgggtcat ctgctgcacg ccatccaccg
    41101 tgacagccaa gggtggcagc gtatgtgtgc ccggggaacc ggcagcaaca ggataacgcc
    41161 aactccggga ttgaaccacc gttgtgttgc cgttgatgct ttggatactg gtggaagact
    41221 gggtggcggg cgctccggtt ttgaaaccga ggcgatgcca gggactgaga tccggctcct
    41281 ttacctctga accgctcgct gaaatcgtaa tata
//
LOCUS       DAOPWB010000009        38514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_994_length_38514_cov_5.967966, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000009 DAOPWB010000000
VERSION     DAOPWB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 38514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38514
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_38514_cov_5.967966"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKN92_01910"
     CDS             <1..431
                     /locus_tag="PKN92_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47578.1"
                     /translation="NYKASQGGDLRCAFSLKLNHSAFDAADATRFGWARSLDMTPGVQ
                     NVILSESSPENAQTSMIMLSDKSVILSELLPLPGGKRMLARFYNPSPVAARTTLKIRG
                     VTKQKLYETDLFGENGKEIPAVMPFSVPPHGIKTLVLEAK"
     gene            complement(460..1305)
                     /locus_tag="PKN92_01915"
     CDS             complement(460..1305)
                     /locus_tag="PKN92_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47579.1"
                     /translation="MKRLLRLAGGGHTPKEMRWHLACLTGGFWGLVLLARLGYPAENH
                     YAITKEMLSALGSFDARYNPRWFWLFTLAMTYCGLMMIPVMLYVRRQLRDLSPIGTFV
                     GTIFFIGGCLSIFLTGLFPYSRAALWGSWRWADIHVPVAGAIAVFFSLGAFWYAALIL
                     KDRFGKKQFTPVPFWGYSVFFLPYLVCFSVFFILGLKLRWDLVYQALQLLFQAELAAA
                     SSFVMAAVKPVQSAAILEHISIWGLTFFVVFFAGVIPDQDGATPEKETGIRPAEGEPQ
                     VDARS"
     gene            complement(1346..2767)
                     /locus_tag="PKN92_01920"
     CDS             complement(1346..2767)
                     /locus_tag="PKN92_01920"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNZ47580.1"
                     /translation="MRLLKKILLGVVVLLLLLASAFLLFIGPWPTYSSSFENAAYFQR
                     NLAQIDTVAAKNDITGEPGQLLCGWGVANLNPPAGVPLAGLSARRGEPSTGIHDDVFV
                     KAVAFSDGKDTAVLIGSDMLLVPTNVADMVREAVAKETSLTAKDLFFTASHTHDSVGG
                     MAPGLVAAISFGPYDPQVPGILTAAFTRAILEAVRSLAPAKFVHGAADATQYIRNRTR
                     DAGVDPLLNWMVIEKESGERCIITRFSAHPTILDSDNMQISAEFPGYLQRKIEEETGT
                     TAVYLGGAVGSMSPRAPEASSDYGKCEALGHALAQLVLEATREELDFSDRADVASVGV
                     SLELPPLQVRLFSPNWRLSPLFRYLVGLSNRGWMGAVKVGDALFVNTPGDFSGEIAAD
                     WQNWASRKGISLWVSGFSGDYAGYISPDRYYGDLFDKKGGLAYETGQMSWLGPHMEAF
                     FTTLMEKMVGTMQLPVQAQAAAF"
     gene            complement(2880..>4191)
                     /locus_tag="PKN92_01925"
     CDS             complement(2880..>4191)
                     /locus_tag="PKN92_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ47581.1"
                     /translation="LTGLLFPGISLLSFTLFFLLQKHACFSEQFRKNGLLWNTLFWLL
                     LMSGLIVGRPPLPQSPSDAAVILPGATLSGQPLLLFALFLSPVIVLNMRRAQAVVSFA
                     DPRQALLRIFPGFLLLLSVSLLLILTNVWGYVPGGEWLRNRFYLPFLPAGTALFILWL
                     KKSTHRPNALRQPLMYKAALSFLVLFLLMQAWQGYRRAGHAPGHENRMSVMTYNIQQG
                     SEQDGTIGYQAQLKVLKHCDADVVALQESDTARPSGGMADPVRYFASSLGYNACYGPG
                     SIIGSYGTALLSRYPIRSPQIRLTYSTVDEVATLSAELAVNGGHVFVSSNHPAGPHEV
                     MSAHAENLAASGSEYRHAIALGDFNFTRKSPYYKRVHQSYPVSAEEKADLSDPENPEQ
                     QRDKIIDHIFASASFEVLESYFLSPPASKSDHPVHWAVLQRGAD"
     assembly_gap    4192..4201
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4202..4896)
                     /locus_tag="PKN92_01930"
     CDS             complement(<4202..4896)
                     /locus_tag="PKN92_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47582.1"
                     /translation="MTEDRTEEKKKNALQQSTSTCFFALLLLFFFQLFGRWIESIYRL
                     SLIKLEPGYEIFGLFLFLAAGLLWLIPRRWDRQTLRFCLVFFFLCRLLTPFLSTPGQV
                     LLSGMGVAVWLIIYAFLLSRQYCSIQGDGGSGLALAVLILVLLRSAGNSIDLSMHIRP
                     FLLGVPLTGAALLLSHRVLASLPESGTPDELPAYPGNTMALLSLFGNYALISLFFSCP
                     LAVCCWHGYHSLFL"
     gene            complement(4893..7247)
                     /locus_tag="PKN92_01935"
     CDS             complement(4893..7247)
                     /locus_tag="PKN92_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HNZ47583.1"
                     /translation="MKNKKCYWYFLLLLGLVYFPPANAVAETVELIREFRFDADNAAD
                     AWKAEGAALFQKDGLYTGSMAGKVFHGVSSEEKFDIPAARDGYLLRVEWTLVPLRIGG
                     WGQHVNLGGPFVVELTGNYATLNARHRAVTAVKAGEETVISCDFNKYQVFDWRINGEP
                     QLEQAVPAWKEGFTRSGLALSDFDQSKSAARWLAVRISKVVPDEVLPLMLSGLDDLSE
                     AKQNEAVPFFTAAASPMDKVFREASDFKGKLDANVCITAAGRESESFQIVAIPAGASL
                     HRLHAVTSDLVHSDGVTRIPSGQVSWHPVGYIQTRESNSSIRRAGWWWPDVLMPPEAV
                     DVKSGYVQPLWFTVAVPHGAKPGDYRGVITLKAEEGERYVPLRLTVRSFSLPLRGKLK
                     TAFCFSPGIWEMWYAPQAVKERMGLTGRSNHGPLYSSSECEDILSREDWYRLYDFLLA
                     HRLSPTQIYSRIKNGRSRVIPPAEHMDYCYERGMNATCLTNVDVLSGDPETADRQMAE
                     LEAWIRSWEECITANDWPDMCWYVHGFDESEMREDPVNTVDPSIRRVFGMLRDQFPWL
                     KRESANPINEKHYGLINVWTPLTQQLPAEKMELVRERQKAGDEVWAYVCCGPGRPYAN
                     LFIDFPGVDPRILPWQYYQKGITGFLYYLINLYDGQENWNTEEPKWPDRPWNPYSFKT
                     NSDGILMYPGPNRMPLASTRMENLRDGIEDYEALAMLEEIARRATEEGNLQDYAERAR
                     AVLAVREEVTASWTDYTQDHEVIVRARREVDALIEEGMQLTGAL"
     gene            complement(7237..8256)
                     /locus_tag="PKN92_01940"
     CDS             complement(7237..8256)
                     /locus_tag="PKN92_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47584.1"
                     /translation="MFKILRILFRLVVVGLLLAFYAYHNGIQYTLAFLTNSMRNPYFD
                     SSSYPPELNCFADETCSSGDLRVLCYNVLCRMCEPEHPWEERLPHLQHLISRYAPDLF
                     GSQELGGWKDIKELMATVEGQYETVSFTFGPWVYADSALFFKKQRFELLDSGQFWLNP
                     KHNLPFGFAWLKLSVPRYVCWACLRDKKTGFTFLFMNSHFDNNSTNKETTAPLVYEHF
                     AKHAKQLPIIFTGDFNTNPTTERYGRLMRGNEGAQVFQNSADLVSVKEIQAYEKGEPL
                     PRAPFPAFNDAIEHIFVAGPAKIVVDEWVMDYNSYGDPQRDASDHPALFAVLRFDLRE
                     TAHEE"
     gene            8437..9306
                     /locus_tag="PKN92_01945"
     CDS             8437..9306
                     /locus_tag="PKN92_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ47585.1"
                     /translation="MKRRDFLGIAGGALATAALSAYGQNAPLALRTVSYNILAFRGFP
                     RTEGNRDALGAAHSRLPEITARTLAAFKPDLITFKEAPYEKKVKRFAEALGMNYAFFP
                     GGWKGDRTYPGGFPGAIITRYDITYSENRPSAGEAHPDTLYTRHLGRAELSTPFGKLH
                     VITTHFHAHKQEVRLEEAQKIIELIESLRTTAPVLLQGDLNHTPDAPEYQLWKNAGLI
                     DIGAKMGIGDEPTYPVTEPRIRIDYIWATPDLAEKAKDAAVLSKPPFVPDPKNPASFA
                     LSDHMPVMATFSD"
     gene            9389..10618
                     /locus_tag="PKN92_01950"
     CDS             9389..10618
                     /locus_tag="PKN92_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47586.1"
                     /translation="MKGQILLLLLPVLAATASEEHLCEAAVPLTALTQTPAAHWFGYY
                     DKFQFDPSDRYVLVMEVSFEDRAPTAEDAVALGMIDLQENNRWIPLGSTTAWCWQQGC
                     MLQWLPGSDSEIIYNIREDQDYAAVIQNVFTGEQRRLPKPVYTVNPDGTEALGLNFTR
                     LGDTRPGYGYNGFEDPPGQELYPDSEGIYRMDLRSGEWKTIITLNQIALAYGNETTDQ
                     GKHWFNHLLYNPDGSRFIFLHRWHKENRKGRFTQGFTAAPDGRDIFCFNDNGMVSHFI
                     WRNTTEILAWSAEKESGNKFHRYFDRSNQVEVVAPDILLQDGHCTWSPCGNWILTDTY
                     PDKEQKQTLFLYRPADGKRIDLGRFYQERPAEEQLRCDLHPRWNRAGTQICIDSRCSG
                     QRQLYLLDVSDIVRNQE"
     gene            complement(10672..12816)
                     /locus_tag="PKN92_01955"
     CDS             complement(10672..12816)
                     /locus_tag="PKN92_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ47587.1"
                     /translation="MKQHNFGWSGLLTAVLLCTMVASGSAAADAFGWRTGAAAWSFRL
                     FSFFEAVDKASELKMPCIEAFQGQKLMPESELTLDYALPEEYFSRIHEKLKDAGVILT
                     SIYIHQIPGEEEACRRAFDFAKALGVSLIVSEPEPEALDLIEKYCNAYGISVALHNHP
                     EGSSRYWHPDVALEACAGRGPRIGVCGDTGHWIRSGLKPADMFRLLKHRLLSVHLKDL
                     EKIDGAWVDVSWGSGSGALEEALSVLADEGITPLLFAAEFETKWENNLPEIAQGKEWF
                     ETTVRRLATAANEKNSLSTGWAVVDITPPRPVALMGQYQTRISEGVQDPLTLTALALE
                     TKKDGNKTEQALLLSCDLCFVESGIVRRIKTALKEQLPDLDSEKIILSATHTHDGPAL
                     NDETIEGVYDTSGVPDIMTASEYGAYFVEKAVEAAGSAWKNRKPSGVSWALGTAVVGI
                     NRRIQYFDGTSVMYGNTARDDFKNFEGSADPGLPLLFFWTPEKKLEGILVNLPCPSQE
                     TESLMVLSADFWHETRLELKKRFGENLYVLAQCAAAGDISPHRTFRKRAEEIMLERKG
                     ISHRQEVANRIANAVAEVLPGAEATIDYEIAFRHVIRMLDLPEKEPPSPPFVKGESVK
                     PAEFHMLRIGDVAMASFPFEYFLDYSLRIQARAKALLTLTVQLANGQSGYLPTDKAVQ
                     GGGYSAENYIVTPEGGQQIVDETVSALNAFWP"
     gene            complement(12813..14117)
                     /locus_tag="PKN92_01960"
     CDS             complement(12813..14117)
                     /locus_tag="PKN92_01960"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47588.1"
                     /translation="MKRKMNRRQFLGSACAVGAGFALPQIVSASVTGQSAAAAPSNTV
                     TLGCIGVGGMGLDNMHKLMSLNNCRVLAICDVYKDRQERAKSIVDEKAGNSDCAVYGD
                     WRELIARDDIDAVMIAAQDHWHALMAVEAAQAGKDIYCEKPLGVSVQESQAIRDAVRK
                     HGCVFQTGTWQRSLENFQRACTLVRNGYIGRVHTVEVSVEGPHYQPKYKGPYGMQPVP
                     EGFDWEMWRGPASPEPYNRGRVEWPDWYLIFDYCVGFITNWGVHHLDIAHWGCPRIGQ
                     EPFELSCEEVIRTEGFTDNSLSWRAEFRYADGFKMIFTDEEKMKLGCKFIGEEGWVHV
                     DRSGIWAEPDSLLTLPFKDSDERLTDSTHHGKNFLDCIATRKDPVSHVDAAHTASILG
                     MLADIAGRTGETLRWDPALEQFINHDGANAQLKRAMHNGWSL"
     gene            complement(14180..15496)
                     /locus_tag="PKN92_01965"
     CDS             complement(14180..15496)
                     /locus_tag="PKN92_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47589.1"
                     /translation="MPKSRKGYGDQVMNESKGITRRTMLAATAAAITAPTILSSVKAA
                     PDPVRLGHIGAGVRGWDLLQHTGSLKSAQVVAVCDVYKPHLERGLKAARNPEAKGYTN
                     YHDLLNDPQVEAVVIATPDHWHEQMVLDAVAAGKAIYCEKGLTTSIAAAKRMRDAVKN
                     SNTVFQLGHQGRQYPSTEEAGRLMREGAIGPVTLVHTGRVFNGTRERAPWRWYGYYNI
                     YDRPDPVQVAKDVDWEAWLGSCPSIDFNEEHFWHWRCYWAYGTGQAGDLLSHELDHVQ
                     SVLRWGIPDSCVCSGHNAFYHDGREVPDTWLAQYTFEKEDAMLLFEGCMNSTREQPPE
                     YIGREGRIIFNGIGQDANRFYVYPDAPPYPHLGRELKPKQVFDPAEHENWPTHMEDFL
                     QCVRSGGTPRCNIDEAFIEAATLLMSVEAYHQKRQVRWDAQIEAIV"
     gene            complement(15572..17881)
                     /locus_tag="PKN92_01970"
     CDS             complement(15572..17881)
                     /locus_tag="PKN92_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47590.1"
                     /translation="MLSMNALFAIMRNEMIIGYGMLAFFTCLCFGYALLYFYKHKNEI
                     GWQELILWCLYGGVFVCLVVPMATRLAFTQVWVQYLTQTRRLTEYLIFIPPMIVLTLF
                     WLAGARILARKNLSHLLFLLLFFLPVFFLFRFLFLYFFDAKPYSDFETYWLMAQENVQ
                     AGSFLVKERLGVSQFWLPYCRRVAVIWTPLIWFFGAAPAVYKSANVVMQLMAFLMAYR
                     IAVRHFGKTAAQLALWLILFVPELIFSCNIPSHDLNGMFLLLGYFFLLDEAYALWGKK
                     KKIPAVVLALFCGILWLAVDLQRSHGPFIFLCSISFVGLHLAFHLPDLRKRELWKTTL
                     LRYLSLFLLIFLLPWGSKTVSEYYVTEATGLRGNAATGGQKHYHTSIDNWNGMFQSLT
                     TTKNYFASLQKSADPPQSNLFWSEYYRDVFLSGYLHNHVRYIPQLMNRASGLLWLGKD
                     VVYYFSRLKPESPIAPHYSLTRGFYFLSDCFMLVFLLLGIFGVTSALKNRDWKINSVQ
                     PLLFLSALVILLLFFGEIQARYTYPIWIILPLYLGVPMKRWLRREKKACPQKSHLLWG
                     TTITTGGFFAFFMSTMAVYALAGNPLLSMQNWEVQASERFTQEELYAPFMQSDGTVQI
                     KFENNWNMTPGLAYTANNLRLQLRFPAQPVPGMYTKAAKSFSLKPDTSYILQGVLECA
                     SQEKLKSLDVDTFYVTVSCNGSTLFVIDYKKIFGIRFVRIPGITADENGKAKIEFRIS
                     AKKSCAENDWKTLSSLSFSHFQLVPAEAI"
     gene            complement(17940..19082)
                     /gene="rffA"
                     /locus_tag="PKN92_01975"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(17940..19082)
                     /gene="rffA"
                     /locus_tag="PKN92_01975"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNZ47591.1"
                     /translation="MSSSSLPIPFNRPGYTGREIAYMQEAMQSGHLSGDGAFTKRCHA
                     FLEERYACKKALLTSSCTDALEMAAILCRIAPGDEVIAPSFSFVTSVNAFVLHGARPV
                     FADIRPDTLNLDETQVETLITKKTRAIVAVHYAGVGCEMDALMDLARRYQLPLIEDNA
                     HGLDGKYRGRYLGTFGSLATQSFHETKNITSGEGGALLINDASLAERAEIIREKGTDR
                     SRFFRGQVDKYSWVDVGSSFLPSELTAAFLYAQLEALDSIQRRRKALWLRYHDGLRDW
                     AEKSGVGLPCVPEHCEQTYHMFYLLMPSLETRQAFIAHLKEQGIQAVFHYLPLHLSVM
                     GKEFGGQEGACPVTEDVSDRLVRLPFYSDMTPAEQERVINAILQFN"
     gene            complement(19166..20182)
                     /locus_tag="PKN92_01980"
     CDS             complement(19166..20182)
                     /locus_tag="PKN92_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ47592.1"
                     /translation="MTADQRKKIVFWLRLTALLLSIAVLLRMVKVQLVLSHIRDIPWY
                     IFTVLVVIAIFRTWLNGLRWQLLNPDASRQLTRWQYFRFLMIAHAFNLIMPGALGGDF
                     VKTAMTLKTVKSNRASNLIAIVADRYIGLFSIIMLGFISLIFATRIPDRMVFYRYFAV
                     LMALFAGLSLISTQPWLFALLEKIFLRLGKVGSALNSLLDTWRGALLYFRKNYFRVLM
                     AFLLCLPIHGVVFITKYTVARYLGISVSFFDICVVQALVWVITAVPVTISGAGVRELS
                     VIYFLSFYGIDAESAAALSVYNYIVNVILGFIGFLFLIDSRGLRRKKAAEDTQTKEQI
                     PDVF"
     gene            complement(20179..21120)
                     /locus_tag="PKN92_01985"
     CDS             complement(20179..21120)
                     /locus_tag="PKN92_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ47593.1"
                     /translation="MNAASTPLYSVVIPVYRSEPTLGLLCRRVFQVFEQMSLAVEIIL
                     IDDSSPDASWQVMQELRHKDNRIKIIRLARNFGQHNALMCGFAHAQGEFILTMDDDLQ
                     HPPEEIPKLIDAMQKDSNCDVVIGSYVAKKHSFIRRLGTRAMQGFNRLALHSEPKLEL
                     TSFRLIRRFIIDELVRLQIDKPRVGRLLVDLSAHIVNVPVAHEERRYGRSGYSFFRLV
                     RDFFNNIVSTSAAPLKVVSVLGFFSAAMSFLLAFYYLYRYFAIGISVPGWTTLIMVNL
                     FHFGVLLFSMGVIGEYLIRILREEQQLPQYFIRDKEL"
     gene            complement(21117..22433)
                     /locus_tag="PKN92_01990"
     CDS             complement(21117..22433)
                     /locus_tag="PKN92_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HNZ47594.1"
                     /translation="MNCKPGIYGVSTQSGRAFFADFLSKGLQSYGYARATEHGRAAVS
                     CFNARGGIYLERPPNNIGEESRFIPLGMSSVGHSLDELLTRSDFIFLSHPSQHLEESV
                     SLLKEAGLMQRQIPLVLTPPRTLAAPYLWRIVGENYPLICFSTSPYSAKALENGVYIK
                     RRKRSWMVSLEGEVSEEQREQISEIFPQVLFNRVPATTSLGNIGAVFHPGGYLLNYDD
                     IEKRQRQGEPYSFYMEGIAARPEVGELLEEIDQIRLRIAHKLNMPVFGLYGNDREEEW
                     QAMMARLRLAESRTEKRDIQTLRKMRHECLSTIHNAIVSAQHWLDYSYGVARIPGESL
                     ANAVSRTPTYQKRSVPQRRYLDEDIPTGLVPLEALAKRFDIDCTAISRILDLYGEKEG
                     KEPRASGRNLQPFTTEYLIRYLKGCPDEEMPDVSMPDTNSTIRSYL"
     gene            complement(22478..23674)
                     /locus_tag="PKN92_01995"
     CDS             complement(22478..23674)
                     /locus_tag="PKN92_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNZ47595.1"
                     /translation="MKKTLFIFGTTYNQIPLVEKAKASGYMTVCLGTGGGVCEKYADK
                     VYPIDFTDIEEVLRLAKIYQPAGMVTCGTSTAIHAIAQCNHLLKFSDKVPPLQVVENC
                     VFKNRYRDIIGESMAPGFSASDPEKAVEMVQTLRLPVVVKPADGGGGKGISLLEKVSL
                     QQMQDAFQHARSYSKMNTVVVEEFIDGTVLGVESFVHDGKIFTLVMPEKLIDEEVMNL
                     TRGVVFPANFSAEIADQIRRVNEAAIRAFGINWGAVHIDMILERNEVPRIIDVGPRLA
                     GGPLMAEMVPRYYNYDFYKATIQLSVGEQPLPVPDQHDNQYYASYSLVPDKDGILESV
                     EYEAEAVDACRVFFYRQLLADGSSVKSPKSDGDRVVLFASTGENYQQTLSHVMSFASG
                     IRMKIR"
     gene            complement(23682..25508)
                     /locus_tag="PKN92_02000"
     CDS             complement(23682..25508)
                     /locus_tag="PKN92_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47596.1"
                     /translation="MSLYFIGATLGNDPHTEGLFKAQRIANLACISGHITAPADTLSL
                     LLGAVREYRPSHIGLSYRLSPDKAEAVFTECIHTLEENGLLRRGDGTWCRVAFAALPE
                     SIARLRARSNQFPVPVEFFSQELPPLDRAEQVLDYFEVRDFRRDRILALLKEELCPPG
                     IEMLDQMAKEVIAGDKWRSEPGLQKPSAEAVKDLIRRFEESELPCLRTHFGVPEDSIQ
                     PTVEGIAALANAGAVDEISLGSSDLSQRYFGEPEAFKGRKNDGGVPYKTVDDLKVLYQ
                     ATRRGNYPGIKPYAHVRNLCAFADDCLKVGMLQGAHQAVPLFWFNELDGRGPLTLDES
                     LVEHQETVAHLARRGIPVEMNDPNHWASRWAHDTVVVADYGLITAVMEQCGVADMIFQ
                     FQLNKPAETGDYADLAKMGAALELVGRLKGNARAWRETRAGIEHFSPDPAEAKYQLAR
                     TTLLQMILEPHMIHLVSYCEARHAATVQDIVESAQIVRHAARLFKKHAPDLMKYRNQN
                     EVVERKHYLLKEAEYLLHAIARLHPRYEPGSLQSIASMLADPFILEAAVKHRFMAAPG
                     LVHPDYPYPRLVTRPSRHGFFDVYEEEGVQILKEAERLSLQQ"
     gene            complement(25697..27769)
                     /locus_tag="PKN92_02005"
     CDS             complement(25697..27769)
                     /locus_tag="PKN92_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNZ47597.1"
                     /translation="MDYVEGWQQRDLPLAFAMRALFLWQSGGLYAVNPSAEPPACWAP
                     FEESGARLVLFGSEEEFVECGGSYTDPGADGQDEDGSPLVVASVITKDGVPVESIDNN
                     TGTLESEFRITYSTETASGVNLSVVRRIYVQDTRPPVIEPAMLQMDPVALMPYGYRNF
                     EADWTYYPRVFHVHILDCNADWSMIDSGTALDACGSEGPTDYWDSVEKTVRRLKPDYS
                     LEYDEFGNEIIVPLDQFTQIPGIYRIDYTVQDSAGNEGRYVTNNFLRYIKVMDTTRVP
                     LVVDAGLTREEAEARILAAHLVPVVIQQHDDNIFADQVISQSPAPDIFALCNSEVQIV
                     VSLGPCLLPTVTGMSLEDAQEALRTAGYISGSVTAVPSEAEAGTVLFIETDNFLCGGV
                     IHLVVSEGPCFMPDVIGLPMSEAIAQITGAGFPAPEIQYDWNVVPVDTVYDQFPDPGK
                     RTCDFQSVLKVSLFLKPTYIDPDASLFNSFECGVYWNDPEASVRVGEEFDPIAWIGAA
                     EIAYQSGESWIPADPEIPLLADHSPYRVTYRYEYRQPYTEEPGVLEGIRFIAVNDTLP
                     PVLTVKDNPDYLLNETIPVYVCRKGQYHHWDDVAAAYDIRILSLEDQCEGSLSADNVV
                     IDIYRYFPDEVNEDDPGEPVLRSLEEAGLTETTWLNTPGFYVVNYSARDQAYNMIFSG
                     RGVEVPED"
     gene            28307..28855
                     /locus_tag="PKN92_02010"
     CDS             28307..28855
                     /locus_tag="PKN92_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47598.1"
                     /translation="MADDAPLPQVLNGELDQWNNGRLAGWIYVPYSALEIQKSQEIYN
                     GHPVITMHHPKLGFLEQVFADGSTLMPGDILSVSVSLRSQPGATVDLSFMLLYGKGDD
                     LQRKYKKEIYTGGGEWQRLEVLWVVSEADLENLEKVVIRLSVANAEAPVSVSHFQAYA
                     ANVGVSANPIPPGSVEVAWIRP"
     gene            28840..29640
                     /locus_tag="PKN92_02015"
     CDS             28840..29640
                     /locus_tag="PKN92_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ47599.1"
                     /translation="MDTPVTLIRNHRLLLWRFALRGLTQRYKGSLFGLLWTVITPLCM
                     LGIYTFMFSVVFQARWGHGVGDSKMAFAIVLLSGLTIYQIFSESVNASIQAVSNNPNY
                     VKKVVFPLEILPFAGHISFLLPNLVWFLMVVAGTWLFMGGLSLKILLLPAILIPLSFL
                     TIGISWFCASLGVFIRDLNHFITIVLRMLLFLTPIFYDTDRIPHPWNYLLLCNPLAHF
                     VDQTRRILIYNQWPDWYVLAGISLFSLAVFQLGYLWFMRTKKGFADVL"
     gene            29630..30931
                     /locus_tag="PKN92_02020"
     CDS             29630..30931
                     /locus_tag="PKN92_02020"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ47600.1"
                     /translation="MSSDPCALNLQNVSKVYHIYEAPHHRLLQMIYGYRRRFFREFKA
                     LDDISFQVGRGESVGIIGRNGAGKSTLLQIIAGTLSATSGNCQTSGRITALLELGSGF
                     NPEFTGHENVLLNAAILGMSEKEIAAKYDDIVAFADIGNFLEQPVKTYSSGMKMRLAF
                     AVQAFIDPEILIIDEALSVGDGAFQMKCMQRMREMIEQKTTILLVSHSLQTLRSFCSR
                     VVWLDQGRIKMDGDTREVTGRYSEVLFGTAVQKQKEKADPEQNIREAGPSPDFPVINL
                     ETASRTETLRRWGSGEAKVTAFSLHDDSGKMSKVYEFGVTLQLNIEAEVIDELPDAFF
                     SMAFAVKNRQGLDIISISTDRLGIFTDPPDTGSKIKVRFTFPLQIAPGDYSLVLAIAY
                     THEGRRTYMDYVENAWYFSILSDRPRYGIYEPDATAVFLDT"
     gene            30948..34079
                     /locus_tag="PKN92_02025"
     CDS             30948..34079
                     /locus_tag="PKN92_02025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ47601.1"
                     /translation="MDTQPGINSPEYWDQRFQQDWSANGGESYTRAYGELLKEHLPGF
                     FFQEMCREQMTVCDWGCAEGEAVAYLSQLYPELDISGVDVSATAIDNARKKYPSLKFA
                     AENWLDPNRELPAYDVVFTSHVLEHFVNPLEIIQQTLARIARTMIIILVPYAEDLDRK
                     DPEHFFRFFDSNLPVRWPGWVCTHFQVLDTRMRAGLCWRGQQALLVYAEENWAKKANP
                     ARCLNSFRGEAAALLPEVKLLEQEKQLLEQEKQLLEQEKQLLRAQEEQLLEQEKQLLE
                     QEKQLLEQEKQLLRAQEELAKRKLEIQYLHDANLMRVNDTLRSKISTIEENLKKETAE
                     HKNLQKKYALLSNDAETLQKELKAQKAESAQFASRNEQLSQDLARAESNYLETKMILH
                     SPGKIALYYLRALKRRLGAGSESPAQKTRPLSSDAPHTDEEQSGRIPSEKTASSTSPK
                     ATAPEAKEIRVAPGARQIRNKDKALKGLTVACILDTFSYACFAPEANFIPVTPHDWRQ
                     TFQQEEIDFLFVESAWNGNDGAWQYRIASYANPAGNELDDLIQWCNTQDIPTVFWNKE
                     DPPNFDRFIRTAVKFDYIFTTDENCVPAYREKCGHDRVYPLMFAAQPAIHNPVDAKWG
                     YRRNVCFAGTYYADCFPERRRQMENLLAGSVPYGLDIFDRMHGYTGEDRNRYIFPEQF
                     QPYIRGRRSYEEMLEEYKRYRVFLNVNSVQDSPTMFARRVFELLACGTPVVSTPSLGI
                     EKIFGELVPQVSTVEETRKALELLVESDEVWHRRSIQGMRKVMAEHCYEHRLAEIAAK
                     LGSGSAPFKPTIHLILEVGAGFPERIIEEILSQHYLPSRIFMFQGTPGETLRKKAFER
                     WLEKTGCNAEEFPYSLCSDEEAHEKIRTALTAAGISVASYERKKTIEKLQQDNSQDYF
                     ALWDGKSFYGPHHLSDSVHALHFSGTSCVGIYYCFRINGIGEFCTPPPSVYDWKNSKY
                     SKKNPHDEGFVFPASMVGKMEGLEEFFVERLPVDEPVGVTADSIIRYPYEFVYSSRNP
                     EPLPHEIRAQVFI"
     gene            34051..36402
                     /locus_tag="PKN92_02030"
     CDS             34051..36402
                     /locus_tag="PKN92_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ47602.1"
                     /translation="MKFVPRCLSDLFKSSPKKTIRSEAARPVRFLLAGHDLKFTGPFH
                     DYFSNNPRYEFRIDEHPGHEIASEKQSRESLEWADVIFCEWALGNLVWYSKHKKPGQM
                     LLARLHSQEWNYRDRLDYIYKTDWNRVDKLLLVTPVIYDHIIQEFPQLGRGKAQVLNC
                     PIDTDAYVKAGQSARKKYALGFVGMVPAMKRLDLALEMLEILQREVPEFSLHIKGKRP
                     EEYPWMLQREEEMRWYEGLYSKIKELKNPDSVIFYEHGSDMPEWYASVSFLLSTSDHE
                     GCHLSVAESMLAGCVPSIRSWDGANRIYPPRFVWSTPEEGAQKILALFEENAREEASA
                     FSRTYAEERFSQGHLCEQVDSMIMRHFETVWPAVVSGECREISSRLLPNILFVGYLAS
                     SSQSGYRIRIEQEIRALARAGARIHFACCHPEMNSAVLNALKVELDALPCPVSFHRAP
                     SFFDMKLTAAEVKALLDSLEEVLQKKRIDILHCEALYCARIGTMLKERKPELKLFFDC
                     HGISPEEEAMGGAAKTRVEKVNQLEMEVLFRTDLNIFVSEAMQQHFRNKHAYVGENYW
                     ILPCCVAESQFASDREETEQRLSLPQNALILSYAGTLAAWQCGEEMLRLFSLLHQKES
                     RLHFLLLLPEKEQEKAREWLHDYGVSDESVTMLEARHDEVPLYLQEAHAGLLLRKEDP
                     VNQVASPTKFGEYLAAGLPVLLTEGIGDFSALVEKEGLGMIVPSSLLDTTEANDEELE
                     QILQFLKQTVSGRDQMRRRCVHYARTELHWERHAHDFLKAITR"
     gene            complement(36415..37434)
                     /gene="rfbB"
                     /locus_tag="PKN92_02035"
     CDS             complement(36415..37434)
                     /gene="rfbB"
                     /locus_tag="PKN92_02035"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNZ47603.1"
                     /translation="MQKILVTGGAGFIGSCFVRTQLRNYPDQHVVVLDKLTYSGNLDN
                     LKGLDETRISFIRGDVNNPEHVRHALEGCDGAVHFAAESHVDRSLSGASDFIATNVGG
                     VNTLLSQARAMNLERILLVSTDEVYGSVTDGSSKEDSPILPRNPYSASKASGELIGLS
                     YFESFCMPVIITRGSNTYGPYQYPEKVLPLFISNAIDNEPLPLYGDGRNVRDWLFVED
                     HCAGIDCAFRKGTPGEAYNIAGENERENITLTRKVLSLLNRPDTLIRYIEDRTGHDRR
                     YSIDSTKLKALGWEPKMNFDEGIAFTVKWYQENEWWWRKIKSGEFKAYYEEHYRERLD
                     KSQGI"
     gene            complement(37496..38395)
                     /gene="rfbA"
                     /locus_tag="PKN92_02040"
     CDS             complement(37496..38395)
                     /gene="rfbA"
                     /locus_tag="PKN92_02040"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNZ47604.1"
                     /translation="MKGIVLAGGAGTRLHPITVAVSKQLLPVYDKPMIYYPLSMLFLA
                     GIRDILIISTPHDLPLFKKLLGDGSQFGIQLSYAEQPQPHGLAHAFIIGEEFCSGEPV
                     ALVLGDNVFYGNNMSAILQKAAKIQEGALIFAYYVSDPERYGVVEFDTSNKAVSLEEK
                     PEHPKSNFAIPGLYFYGPDVVSLAKNLKPGKRGELEITDLNRIYLEQQRLQVSVFGRG
                     VAWLDTGTNRSLLDATMFVQAIEERQGLKIACLEEIAFKNGWIDLKAVEKSAKKMGSS
                     AYGDYLRFLVKRQKQKLSQNAPL"
BASE COUNT         9956 a        10108 c         9459 g         8981 t
ORIGIN      
        1 ccaattacaa ggcatcgcag gggggcgatc tgcgctgtgc cttttctctg aaactgaacc
       61 acagcgcctt tgatgctgcc gatgccaccc gtttcggctg ggcacgcagc ctggatatga
      121 cgccgggcgt acagaatgtc attctcagtg agagcagtcc ggaaaacgcc cagacaagca
      181 tgatcatgct gagcgacaaa tcagtcatcc tcagtgaact gctgcccctt cccggcggaa
      241 aacgaatgct tgcacgcttc tacaatccat cgcctgttgc tgcacgcacc actttaaaaa
      301 tacggggcgt tacgaagcaa aaactctatg aaaccgatct cttcggtgaa aacggaaaag
      361 aaattccagc cgtgatgccc ttcagcgtgc cgccgcacgg cattaaaaca ctggtgcttg
      421 aagccaaata gccttccacc ggactgaagc acggagtcat tatgaccggg catccacctg
      481 cggttcacct tccgcagggc ggatgcctgt ttctttttca ggggtggctc catcctggtc
      541 aggtatcact ccggcaaaaa acacgacaaa gaaagtcagt ccccatatgg aaatatgctc
      601 gagaatcgcc gccgactgaa cgggcttcac cgctgccatc acaaaggaag atgctgctgc
      661 aagttccgcc tgaaagagca gctgaagcgc ttgataaaca agatcccacc gcagtttcag
      721 tccgagtata aaaaacacag aaaaacacac cagataaggc agaaaaaaca cactgtaacc
      781 ccagaacggc actggagtaa attgcttttt tccgaaccgg tctttcagaa tcagcgcggc
      841 ataccagaaa gcgcccagcg aaaaaaagac agcaatagcg cctgcaacgg gcacgtgaat
      901 gtccgcccag cgccatgaac cccagagggc tgcgcgggaa tagggaaaca gcccggtcaa
      961 aaaaatagac aggcacccgc cgataaagaa gatcgtaccg acaaaggtgc caatgggaga
     1021 aagatcccga agctgccggc gcacatataa catcaccggg atcatcatca gcccgcagta
     1081 agtcatggct agcgtaaaaa gccagaacca gcgggggtta taccgggcat caaagctgcc
     1141 cagcgcactg agcatttctt tagtgatggc atagtgattt tcggcaggat accccaaccg
     1201 ggcgagaagc acaagccccc agaagccacc agtgaggcac gcaagatgcc accgcatttc
     1261 ttttggcgtg tgcccgcctc ccgcaagacg caacaaacgc ttcatgacgc atcacccttt
     1321 tgcccggaag ctccggggct gcctgtcaga aggcagcagc ctgtgcctga accggcagct
     1381 gcattgtgcc gaccattttc tccatcaatg tagtaaaaaa ggcttccata tgaggtccga
     1441 gccaggacat ctgtcccgtt tcataggcaa gcccgccttt tttatcaaac agatccccat
     1501 agtaacggtc aggagatata taacctgcat aatcaccgga aaagccggac acccagaggc
     1561 ttatcccttt gcgggacgcc cagttctgcc aatcggcagc tatttcaccg ctgaaatcgc
     1621 caggtgtatt cacaaagagg gcatctccca ctttcaccgc acccatccac ccgcggttgc
     1681 tcaaaccgac cagataacgg aacagaggcg acaagcgcca attagggctg aaaaggcgca
     1741 cctgcagcgg cggaagttcg agagaaacac cgacactggc cacatcagca cgatccgaaa
     1801 aatccagttc ttccctggtc gcttccagca cgagctgtgc cagagcatga ccgagggctt
     1861 cacattttcc gtaatcggaa ctggcctcgg gagcgcgggg gctcatgctg cccacagcgc
     1921 cgcccagata caccgcagtt gttccagttt cctcttcaat ctttcgctgg agatatcctg
     1981 gaaactctgc tgatatctgc atgttatcgc tgtccagaat agtcgggtga gcagaaaagc
     2041 gcgtaatgat acagcgttcg ccgctttcct tttcaatcac catccagttc agcagcggat
     2101 ccacacccgc atcccgggta cgattacgga tgtactgggt tgcatcagca gcgccatgaa
     2161 caaattttgc cggggcaagc gaacggacag cttccagaat cgcacgggta aaggctgctg
     2221 tcaggatgcc gggtacttga ggatcatagg gcccgaaaga aatggctgcg acgagcccgg
     2281 gtgccatgcc tccgacactg tcatgtgtgt gacttgctgt gaaaaagagg tctttcgctg
     2341 tcagagatgt ttcctttgcg acagcttcac gcaccatatc ggcaacgttg gtaggaacga
     2401 gcagcatatc agaaccgatc agcacagccg tatctttccc atcgctgaaa gcaacggctt
     2461 tcacaaaaac atcgtcatga attccggtgg agggttcacc tctccgggca ctcaaccccg
     2521 ccagcggcac tcccgccgga gggttcagat tggcaacgcc ccatccgcac agcaattgcc
     2581 ccggctcgcc agtaatatca tttttggcgg caacggtatc aatttgtgca aggttgcgct
     2641 gaaagtaggc ggcattttcg aagctgctgc tataggtggg ccagggcccg atgaagagaa
     2701 gaaaagcact ggcgaggagg agaagcagaa caacgacacc cagaagaatt tttttcagca
     2761 aacgcattat tatcatcctt gttgtgagga atatgtttta cgcaacaggg catatcttaa
     2821 aaatactgcc cactcagaag acccgaacaa atcacatccg gtttcgcttt ttcctgcttt
     2881 cagtcagcgc cccgctgcag aaccgcccaa tggacagggt gatcgctttt agatgcaggc
     2941 ggtgaaagaa aataactttc cagcacttca aaagaagcgc ttgcaaaaat atgatctatg
     3001 attttgtccc gctgctgttc cggattctca ggatcggata aatccgcctt ctcctccgct
     3061 gaaacgggat aggattgatg tacccgctta taatacggag attttcgtgt gaaattaaaa
     3121 tcgcccaggg caatggcatg gcgatattca ctgcccgatg cagccagatt ttccgcatgg
     3181 gcggacatca cctcgtgcgg accggccgga tggttggatg aaacgaaaac atgtccgccg
     3241 ttgacggcca actccgccga cagtgtcgcc acttcatcca cagtgctgta ggtcagtctg
     3301 atttgaggcg atcggatagg ataacgggac agcagcgccg tgccgtagct gccgatgatg
     3361 cttccaggac cgtaacaggc attatatccg agagaagaag caaagtaccg gacaggatct
     3421 gccattcccc ctgaaggtcg cgctgtatcg ctctcctgca gtgcgaccac gtccgcatca
     3481 cagtgcttca gaaccttgag ttgcgcctga tacccaatag tgccgtcctg ctcggaaccc
     3541 tgctggatgt tatacgtcat cacggacatg cgattttcat ggccgggcgc atgccctgcc
     3601 ctgcgatatc cctgccatgc ctgcatcagg agaaatagca caagaaaaga taaggctgcc
     3661 ttgtacatca gcggctgccg taatgcattt gggcggtgag tcgacttttt tagccacagg
     3721 atgaaaagtg cagtacctgc gggaagaaaa ggaaggtaaa accggttgcg aagccactca
     3781 ccaccaggca catagcccca gacattggtg aggatcagaa gcagcgatac agagaggaga
     3841 agcagaaacc cgggaaagat gcgcagcaat gcctgacggg gatcagcaaa ggacacgaca
     3901 gcttgtgccc tccgcatatt caaaacaatg acaggagaca aaaagagagc gaacaacagt
     3961 aagggctgac ctgaaagtgt ggcaccgggc agtatgaccg ctgcatcgga cgggctttgt
     4021 ggcagcggcg gtcgcccgac aatgagcccg gacatgagga gaagccaaaa aagggtattc
     4081 cataaaagtc cgttttttct gaactgttca gagaagcatg cgtgcttctg cagcagaaaa
     4141 aacagtgtga acgaaaggag gctaatcccg gggaacagta agcctgtcag gnnnnnnnnn
     4201 naggaaaaga gagtggtagc catgccagca gcataccgcc agcggacagg agaagaacaa
     4261 agagatcagt gcatagtttc cgaaaagact cagcagcgcc atcgtatttc ccggatatgc
     4321 cggcagctca tccggcgtgc cggattctgg cagggacgcc agcacacgat gactcagaag
     4381 cagggcggca cctgtcagag gaacacccag cagaaaaggg cggatatgca tggacagatc
     4441 aatggaattt cccgctgaac gaagcaacac caggatcagc accgcgagag ccagcccgct
     4501 gcctccgtcc ccctgaattg aacagtattg cctgctaagg agaaaggcat aaataatcag
     4561 ccaaacggca acgcccattc cagaaagcaa cacctgcccc ggtgtagata gaaagggggt
     4621 cagcagcctg cacagaaaaa agaaaaccag acaaaagcgc agggtctgcc gatcccaccg
     4681 gcggggaatc agccacagca gtccggcagc cagaaaaagg aacaacccga atatttcata
     4741 acccggttcc agtttaatca ggcttaaccg ataaatgctt tcaatccagc gaccgaaaag
     4801 ctgaaagaaa aacagcagca gcagagcaaa gaaacaggtg ctggtgctct gctgtaaagc
     4861 gttctttttc ttctcttccg ttctatcttc tgtcataatg cgcctgtaag ctgcatccct
     4921 tcctctatga gagcatccac ttcccggcgg gcacggacaa tgacttcatg atcctgtgta
     4981 tagtccgtcc acgatgctgt aacttcttcc ctgactgcca gcacagcccg ggctctctcc
     5041 gcataatcct gcaggtttcc ctcttctgtc gcccgccttg caatttcctc aagcatcgcc
     5101 agcgcttcat agtcttcaat accatcacgc aggttttcca tccgggtact ggcaagaggc
     5161 atccgattcg gacccggata catcagaatc ccatcactgt tcgttttaaa cgaataggga
     5221 ttccacgggc ggtccggcca tttcggttct tccgtattcc agttttcctg cccgtcatac
     5281 agattgatca ggtaataaag aaaaccggta attcctttct ggtagtattg ccacgggagg
     5341 atccggggat ccacaccggg aaagtcgata aacagattgg catagggtct gccgggacca
     5401 cagcatacat aagcccagac ttcatcacct gctttctgcc gttcccgcac cagttccatc
     5461 ttctcggcgg gcagctgttg tgtcagcggg gtccatacat taatcagacc gtaatgtttt
     5521 tcattgattg gattggcact ttcccgtttc agccagggaa attgatcccg gagcatgcca
     5581 aacacgcggc gtatggaagg atccaccgta tttaccggat cctcgcgcat ctcggactcg
     5641 tcaaagccgt gtacatacca gcacatgtcc ggccagtcgt ttgcggttat gcactcttcc
     5701 caggagcgga tccaggcttc cagctccgcc atctgccgat cggccgtctc cggatcgccg
     5761 gaaagcacat ccacattggt caggcaggtc gcattcatac cccgctcata gcagtaatcc
     5821 atgtgctccg caggcggaat caccctgctc cggccattct tgattcttga gtaaatctgt
     5881 gtcgggctca gccggtgcgc cagcagaaaa tcgtacagtc tgtaccagtc ctcccggctg
     5941 agaatgtcct cgcattcgga agaactgtaa agcggcccgt gattacttct cccggtcaag
     6001 cccatccgct ctttcactgc ctgcggtgca taccacatct cccagatacc cggagaaaag
     6061 cagaaggccg ttttcagttt tccgcgaagc ggcagcgaaa agctccgcac tgtcaatcgc
     6121 agcggcacgt aacgctcgcc ttcttccgct ttcagcgtaa tcacgccgcg gtagtcgccg
     6181 ggctttgcac catgcggcac agccaccgta aaccagagcg gctgtacata cccgcttttt
     6241 acatccactg cttccggcgg catgagtaca tcaggccacc accagccagc ccgccggatg
     6301 gaggaattgg attcgcgggt ctgaatgtaa ccgaccggat gccagctgac ctgccccgac
     6361 gggatacgcg tcacaccgtc gctgtgtaca agatccgaag tcactgcgtg aagccggtgc
     6421 aaagacgctc cagccgggat cgctacaatc tgaaaactct ccgattcgcg gccagcggca
     6481 gtaatgcaaa cattggcgtc cagttttcct ttaaaatcgg acgcctcacg gaagacttta
     6541 tccatgggac tggctgctgc ggtaaaaaac ggaaccgcct cattctgctt tgcctcggac
     6601 aggtcatcca ggccggacag cataaggggc aagacctcat cgggaactac cttgcttatg
     6661 cggactgcca gccaccgggc ggcacttttc gattgatcaa aatcagaaag tgcgagaccg
     6721 ctccgggtaa acccttcttt ccaggcaggc accgcctgct ccagctgcgg ctcaccgttg
     6781 atcctccagt caaagacctg atacttgttg aaatcacagg atatcacagt ctcctcacct
     6841 gctttcacgg cagtgacagc acgatggcgg gcgtttaatg tggcataatt tccggtcagc
     6901 tccaccacaa aagggcctcc caaattcaca tgctgtcccc agccgccaat acgcagcggc
     6961 accagagtcc attccacccg aagcaggtat ccatcacggg cagcggggat gtcaaacttc
     7021 tcttcactgc ttacgccatg aaacaccttt cccgccatac tgcccgtgta taaaccgtcc
     7081 ttctgaaaga gggcagcacc ttccgccttc caggcatcgg ctgcattgtc cgcatcaaag
     7141 cgaaactccc gaatcagctc cacagtctct gcgacagcgt tcgccggcgg aaagtacaca
     7201 agaccaagaa gcagcagaaa ataccagtag cattttttat tcttcatgcg ccgtttctct
     7261 caaatcaaaa cgcagaacag caaagagggc aggatgatca gaagcgtccc gctgcggatc
     7321 tccgtaggaa ttgtagtcca taacccattc atccaccaca atttttgccg ggcctgccac
     7381 aaaaatatgt tcgatagcat cgttaaaggc aggaaaagga gcccggggca gcggctcacc
     7441 tttttcataa gcctgtattt ccttaactga cacgagatcg gcgctgtttt gaaaaacctg
     7501 agcgccctca ttgccgcgca ttagtctgcc ataccgttca gttgtgggat ttgtgttgaa
     7561 atcgcctgta aaaatgatgg gcaattgctt cgcatgtttt gcgaagtgct catatacaag
     7621 gggcgccgtt gtttctttat tggttgagtt gttgtcgaag tgggagttca taaacaggaa
     7681 agtaaaacca gtttttttat cgcgcaggca cgcccagcag acatatcgcg gcacactgag
     7741 tttcagccag gcaaagccga agggcaggtt gtgtttggga ttcagccaga attgccctga
     7801 atcaagaagt tcaaaacgct gcttcttaaa aaacagcgcg gaatcggcat aaacccaggg
     7861 accgaaagta aaactgaccg tttcatactg tccctctact gtcgccatca gctcctttat
     7921 atctttccat ccgcccagct cctgcgaccc gaaaagatcc ggagcgtaac gggaaatcag
     7981 gtgctgcaga tgaggcagcc gctcctccca gggatgttcc ggttcgcaca tccggcagag
     8041 cacgttgtaa caaaggacgc gcaggtctcc gctggaacaa gtctcgtccg caaagcaatt
     8101 taactccggc ggataggagg aagaatcgaa atacggattt cgcatggaat ttgtcagaaa
     8161 cgccagcgtg tactgaatac cgttgtggta cgcataaaaa gcgagcagca gcccgacaac
     8221 aacaagacga aataaaatac gcagaatttt aaacataatc cctggctttc tttcccgacg
     8281 gggcggtgtt gcagaaaaaa actgaaattt atactaacac cctattcagg catatttaaa
     8341 ctgtattctt aaggaatgtt gtgataccat gaacttttga ctgtagaaag cgcggttggt
     8401 tttacagccg ttgcgaacga ttgaggagaa ataagtatga aaagacgtga ttttttgggt
     8461 attgcaggcg gagcgctggc aactgccgct ctttccgctt acggacagaa tgcgccgctt
     8521 gcgctgcgga cagtcagtta taacatactg gcgttccgtg gatttccccg tactgaaggc
     8581 aaccgtgatg ctctgggcgc cgcgcacagc cgtctccctg aaattacagc ccggacactt
     8641 gccgctttta aacctgacct gatcactttc aaagaagcgc cctatgaaaa gaaagtgaaa
     8701 cgttttgccg aagctctcgg catgaactac gctttcttcc cgggtggctg gaaaggagac
     8761 cgcacctatc ctggtggctt ccccggtgcc attatcacgc gttatgacat tacatacagt
     8821 gaaaacaggc cttcagccgg agaggcacac ccggatacgc tgtatacccg ccatctcggc
     8881 agagcagagc tttccacgcc cttcgggaaa ctgcacgtta tcacaaccca cttccatgct
     8941 cataaacagg aggtgcggtt ggaagaagca cagaagatta tcgagcttat tgagagcctt
     9001 cgcactaccg ctcccgtact gctgcagggc gatttgaatc atacccctga tgcacccgag
     9061 tatcagctgt ggaaaaatgc cggactcatt gacatcggcg cgaaaatggg tatcggcgat
     9121 gagccgacct accccgtcac cgagccacgc atccgcattg actacatctg ggcaacaccg
     9181 gatctggctg aaaaagccaa agatgcggca gtgctgagca aaccgccttt tgtgccggat
     9241 cccaaaaatc cggcgtcctt tgcactcagc gatcacatgc cggtcatggc gactttttct
     9301 gactgagcac tcccctgttc gcagcccaac cgaagagaga aacgtctgcc cgggactctt
     9361 ttccgggcgg gatgaaagga cagattgcat gaaagggcag atcctgttgc ttctgcttcc
     9421 ggtgctggca gcgacggcat ctgaagagca tctttgcgaa gccgctgtac ctttgacagc
     9481 actgacgcaa acaccggcag cacactggtt cggttattac gacaagtttc agttcgatcc
     9541 ctccgaccgt tacgtgcttg tcatggaagt gagttttgaa gacagggctc ccactgccga
     9601 agatgccgtt gcgctcggca tgattgatct tcaggaaaat aaccgatgga ttccgctggg
     9661 aagcacaacc gcctggtgct ggcagcaggg atgcatgctg cagtggctgc ccggttcgga
     9721 ctcggaaatc atttataaca tccgtgaaga ccaggattat gctgctgtca tacagaatgt
     9781 atttaccggc gagcagcggc ggctgccgaa gcccgtttat accgtcaacc cggacggaac
     9841 tgaagcactc ggattaaatt tcacccggct tggcgatacc cgccccggct acggttacaa
     9901 tggttttgaa gatccgccgg gtcaggagct ctatcccgat tcggaaggaa tttaccggat
     9961 ggatctgcgc agcggagaat ggaagactat cattaccctg aatcagattg ctctggctta
    10021 tggcaatgaa accaccgatc agggaaaaca ctggttcaac catctgctct acaacccgga
    10081 cggaagccgc tttatctttc tccaccgctg gcacaaggaa aatcgaaaag gaagatttac
    10141 tcaggggttc acggcagcac cggacgggcg ggacatcttc tgttttaacg acaacgggat
    10201 ggtatctcat tttatctggc ggaatactac agaaatcctt gcctggtcag cagaaaaaga
    10261 aagcggaaat aaattccatc gctattttga cagaagcaat caggttgagg tggtggcgcc
    10321 agatattctg cttcaggacg ggcattgcac ctggtcaccc tgcggcaact ggattcttac
    10381 ggacacctat cccgacaaag agcagaaaca gacactgttt ctttatcgcc ctgccgatgg
    10441 aaagcggatt gatttaggtc gtttttatca ggaacgtccg gcggaagaac agctgcgctg
    10501 cgatctccat ccccggtgga accgtgcggg cacacaaatc tgcattgatt cgcgctgttc
    10561 cggacagcgt cagctctatc tgctggatgt aagtgacatt gtgcgtaatc aggaataaaa
    10621 aaagaagccg gcacttccgc accggcttct aaaaacaaac agcactacga ctcaaggcca
    10681 gaaagcattc agtgcagata cagtctcgtc cacaatctgc tgtccccctt ccggtgtgac
    10741 aatataattt tcggcactgt agccgccgcc ctgcacagct ttatccgtag gcaaatatcc
    10801 gctctgtcca ttcgccagct gcaccgtcag cgtaagcagt gctttggcgc gcgcctgaat
    10861 acggaggctg tagtccagaa aatattcaaa ggggaaactt gccatggcaa catccccgat
    10921 acgaagcata tgaaactcgg cgggcttcac agattctcct tttacgaaag gcggagacgg
    10981 cggttccttt tccggaagat ccagcatgcg gatcacatgt cggaacgcta tctcataatc
    11041 gatggtggct tccgcaccgg gcagtacttc cgccactgca ttggcaatgc ggtttgccac
    11101 ttcctgtctg tggctgattc cctttcgttc cagcataatt tcttcggctc ttttccggaa
    11161 ggtacgatgc ggggatatat cgcctgccgc cgcacactgc gccagaacgt aaagattctc
    11221 gccaaaacgt ttcttcagtt ccaggcgggt ctcatgccag aagtcagcgg aaagcaccat
    11281 cagggactct gtttcctggg atgggcaggg cagattcact aaaatgcctt ccagtttttt
    11341 ctccggtgtc cagaagaaaa gaagcggaag tcccggatct gcacttcctt caaaattttt
    11401 gaagtcatcc cgggcagtat ttccgtacat gaccgaagtt ccgtcaaaat actgaatacg
    11461 ccggttgata ccgacgacag ctgtacctaa agcccagctg accccggagg gtttccggtt
    11521 tttccaggca cttccggctg cttcaacagc tttttccaca aaataagcgc cgtattccga
    11581 agcggtcata atgtcgggca caccgcttgt atcataaacc ccttcgatcg tttcatcatt
    11641 gagcgccggg ccgtcatggg tatgggtcgc actgagtatg attttttcag aatccagatc
    11701 aggcagctgt tctttcaaag ccgttttgat gcgtcggacg ataccgcttt ccacaaagca
    11761 caggtcacaa cttaaaagaa gcgcctgctc tgttttattg ccatcttttt ttgtttcgag
    11821 cgccagagct gtcagggtca acggatcctg tacaccttcc gaaatacggg tctggtactg
    11881 ccccatgagc gcgacgggcc gtggtggcgt aatatctaca accgcccagc ctgtactcag
    11941 gctattcttt tcgtttgctg ccgtcgcaag cctgcggact gtggtttcaa accactcttt
    12001 tccttgagca atttccggca ggttattctc ccatttcgtt tcaaattcgg ctgcaaaaag
    12061 caacggagtg attccttcat cggcaagtac ggataatgcc tcttccagcg caccactgcc
    12121 actgccccag gacacatcaa cccaggcacc gtcaattttt tccaggtctt tcaggtgcac
    12181 cgagagaagc cggtgcttga ggagccggaa catgtcagcg ggcttcagac cggagcgtat
    12241 ccaatgccct gtatctccgc aaacaccgat gcgcgggccc cgccctgcac aggcttccag
    12301 cgccacatca ggatgccagt agcgggacga cccttcagga tgattgtgca gtgccacact
    12361 gattccatag gcattacagt atttttcaat gagatcaagc gcctcaggtt ccggttcgga
    12421 gacgatcaac gagactccca gcgccttcgc aaaatcaaag gcacgccggc aagcctcttc
    12481 ttcaccggga atctgatgga tatagatgct ggtaagtatg acgcctgcat ctttgagttt
    12541 ttcatgaatc cgggaaaaat attcttccgg aagcgcataa tccagtgtga gttcgctctc
    12601 aggcatcagt ttctgtccct gaaaagcttc aatgcagggc atcttcaatt ctgatgcttt
    12661 gtccacggct tcaaaaaaac tgaacagccg gaaactccag gcggcagcac ccgtacgcca
    12721 gccgaaagca tctgcggcag cagacccgga tgccaccatc gtgcaaagga gaaccgccgt
    12781 cagcagcccg ctccatccaa aattatgctg tttcataatg accatccgtt gtgcatggca
    12841 cgtttcaact gcgcattggc gccgtcatga ttgataaact gttccagtgc gggatcccag
    12901 cgcaaggtct cccctgtccg cccggcaata tccgccagca taccgagtat cgacgctgtg
    12961 tgcgcagcgt ccacatgcga cacagggtct tttcgggtgg cgatgcagtc caggaaattt
    13021 ttcccgtggt gagtggaatc tgtaagtcgc tcgtcactat ccttaaacgg cagggtgagc
    13081 agtgaatcgg gctcagccca gatgcctgaa cgatccacat gaacccagcc ctcttcaccg
    13141 atgaatttgc atcccagttt catcttctct tcatcggtga aaatcatttt aaagccgtcg
    13201 gcgtaacgga attcggcgcg ccagcttagc gaattatccg tgaacccttc cgtccggatc
    13261 acttcttcac aggacagctc aaaaggctcc tgtcctatcc gggggcagcc ccaatgggcg
    13321 atatcgagat gatgcacacc ccagttggtg ataaaaccta cgcaataatc aaaaatgagg
    13381 taccagtccg gccattcgac acgcccgcgg ttgtacggct cgggcgaagc aggccctctc
    13441 cacatttccc agtcaaaccc ttccggcacg ggctgcatgc cataggggcc tttgtatttg
    13501 ggctgatagt gtggtccttc cacactcact tccacggtat gcaccctgcc tatatagccg
    13561 ttgcgcacca gtgtgcaggc acgctgaaag ttttccagcg aacgctgcca ggttcccgtc
    13621 tgaaaaacac agccatgctt tctcactgca tcacggattg cctgcgactc ctgcacggat
    13681 acgcccagcg gtttttcaca atagatgtct ttgcccgcct gtgccgcttc caccgccatc
    13741 agtgcatgcc aatgatcctg tgccgcaatc atgacagcgt caatatcatc acgggcaatc
    13801 agctcacgcc agtcgccata aaccgcacag tcgctgttgc ccgccttctc atcgacaatg
    13861 gacttcgccc gttcctggcg gtctttataa acatcgcaaa tcgcaagaac ccggcagttg
    13921 ttcaggctca tgagcttgtg catattatcg aggcccatgc cgccgacacc gatacacccg
    13981 agggtgaccg tattggaagg cgccgctgca gcggattgcc ccgttacgga tgcggacaca
    14041 atctgcggca gtgcaaatcc ggcaccgaca gcgcaggcac tgccgaggaa ctggcgcctg
    14101 ttcatttttc gtttcatgat atttcctttg tcaggcaaaa aacagcctgt aactctcact
    14161 tccatccaaa aagatatttt cagacaattg cctctatctg agcatcccag cgtacctggc
    14221 gtttctggtg gtaggcttca acgctcatca gaagggtggc tgcttcaata aaagcttcat
    14281 caatattgca acggggcgtc ccgccgctgc gcacgcactg aaggaagtct tccatgtggg
    14341 taggccagtt ctcatgctct gcaggatcaa agacctgttt cggcttcagc tctctgccca
    14401 ggtgcgggta gggcggcgca tcggggtaca cataaaaacg attggcgtcc tgcccgatgc
    14461 cattgaaaat aatgcgccct tcccgtccga tatattcagg cggctgctcg cgggtggagt
    14521 tcatgcagcc ttcaaaaagc agcatggcat cttctttttc aaaggtgtac tgggcaagcc
    14581 aggtatcagg cacttccctg ccgtcgtggt aaaaagcatt gtgcccggag cagacacaac
    14641 tgtcaggaat tccccagcgg agaaccgact gcacatgatc cagttcgtgt gacagcaggt
    14701 cgcctgcctg cccggtgccg tatgcccagt aacagcgcca atgccagaaa tgctcttcat
    14761 taaaatcgat ggacgggcat gaaccgagcc acgcttccca gtccacatcc tttgccacct
    14821 gaacgggatc cggccggtca taaatattat aatatccgta ccagcgccag ggggcacgtt
    14881 cccgtgttcc gttaaaaacg cgccccgtat gtaccagcgt caccggtccg atcgcaccct
    14941 cgcgcatgag acgccctgct tcttccgtgg acgggtactg gcggccctga tgtcccagct
    15001 gaaataccgt attgctgttt ttaaccgcat cccgcatgcg ttttgccgca gcaatggaag
    15061 tagtcagccc tttctcgcag taaatggctt tacccgccgc caccgcatcc agcaccatct
    15121 gctcgtgcca gtggtccgga gtggcaataa ccactgcctc cacctgaggg tcattcagca
    15181 gatcatgata attggtatag cctttcgctt cgggattacg cgccgctttc aacccccgtt
    15241 ccagatgcgg cttgtacacg tcacagacgg caaccacttg cgcacttttg agcgagcccg
    15301 tatgctgcag cagatcccag ccccgtacac cggcacctat gtgccccaga cgcaccggat
    15361 ccggagccgc tttcacggaa gagagaatgg tcggagctgt gatagccgcc gccgttgcgg
    15421 caagcatggt acgacgggtg attcctttgg attcattcat gacctgatct ccatagcctt
    15481 tgcggctttt tggcattgat tctgtcctgt tcagataagt caaaagactg agggttattt
    15541 tgcaggataa acgctttcat gtcaaacggt atcagatcgc ctcggcaggt accagctgaa
    15601 aatgactgaa actgagagag ctcaatgttt tccagtcgtt ttccgcacag ctcttctttg
    15661 cactgatcct gaattctatt ttcgctttcc cgttttcatc tgccgtaata cccggaatac
    15721 ggacaaagcg aataccgaaa atctttttgt aatcaatgac aaataatgtg ctgccgttac
    15781 aactcacggt gacataaaag gtatctacat ccaacgattt caatttctcc tgagaagcac
    15841 attccaacac tccctgaaga atataagaag tgtccggttt taaagaaaat gatttggctg
    15901 ccttcgtata catgcccggc accggctgcg ccggaaagcg aagctgcagg cgcagattgt
    15961 tggcggtata ggcaaggcct ggagtcatat tccagttgtt ttcaaacttg atctgcactg
    16021 taccatcact ttgcataaaa ggcgcataca actcttcttg tgtaaaacgc tctgaagcct
    16081 gcacctccca gttttgcatg gacagcagcg gatttccggc gagtgcataa acagccatgg
    16141 tgctcataaa aaaagcgaaa aagccgcctg ttgtaatagt tgttccccaa agaagatggc
    16201 ttttttgtgg gcatgctttt ttttcacggc gcagccagcg tttcatgggt actcccagat
    16261 ataaaggcag aatgatccat atgggatagg tatagcgcgc ctgtatttct ccaaagaaga
    16321 gcagcaggat cacaagtgcc gataaaaaaa gaaggggctg tacgctattg atcttccaat
    16381 ctcggttttt cagtgcgctc gtgacaccaa agatgccaag caaaagaaaa acgagcataa
    16441 aacagtcgct aagaaaataa aagccccgtg tcaagctgta gtgaggtgct atcgggctct
    16501 ccggcttcag ccgggaaaaa tagtagacca catctttacc cagccataac agaccgcttg
    16561 ccctattcat caattgaggg atataacgca catgattatg cagataaccg gataagaaaa
    16621 catcccgata atactcagac cagaataaat tggactgggg cggatccgcg gacttttgga
    16681 gcgaggcaaa atagttcttc gtagtagtaa gggattgaaa catgccgttc cagttatcga
    16741 tggaagtgtg gtaatgtttt tgtccgcctg ttgccgcatt tcctcttaaa ccggtagcct
    16801 ccgtcacgta gtattcagac actgttttgc ttccccaggg gagaagaaaa atcagaagaa
    16861 aaagcgataa atatcgaagc agcgtcgtct tccacagttc ccgttttcta agatccggga
    16921 gatgaaatgc cagatgaagc ccgacaaaag aaatgctgca aagaaaaatg aaaggcccgt
    16981 ggcttcgctg caaatcgact gcaagccaga gaatcccgca aaacaatgca agaacaacgg
    17041 caggaatctt cttttttttc ccccataaag cgtacgcttc atccaacaga aaaaaatatc
    17101 ctaaaagaag gaacatgccg ttgaggtcgt ggctgggtat attacagctg aagatcagct
    17161 cgggcacaaa gagtatcagc cacagggcaa gctgtgccgc tgtttttccg aaatggcgta
    17221 cggctattct gtacgccatc aggaaggcca tcagctgcat gacaacattg gcactcttat
    17281 atacagcagg agcagcgcca aaaaaccaga tgagcggtgt ccagattacg gcgaccctgc
    17341 ggcaataggg caaccagaac tgcgaaacac ccaagcgttc tttgacaaga aaagaacctg
    17401 cctgaacatt ttcctgtgcc atcagccagt aggtttcaaa atcagagtag ggctttgcat
    17461 caaagaagta gagaaaaaga aaacgaaaga ggaaaaacac aggcagaaaa aaaagaagca
    17521 gaaacaacaa atgagaaaga ttttttcgcg caaggatccg ggcaccggcg agccagaaca
    17581 aagttaaaac gatcatgggc ggaataaaga ttaaatattc agtcaatcgg cgggtttgtg
    17641 tcaggtactg cacccatacc tgtgtaaaag caagccgggt tgccatgggc acaaccagac
    17701 acacaaacac acctccgtag agacaccaga gaataagttc ctgccatcct atctcatttt
    17761 tatgtttata aaaatacaga agcgcatagc cgaaacaaag gcacgtgaaa aaagcgagca
    17821 tcccataacc gatgatcatt tcgtttcgca taatcgcgaa aagggcattc atagacagca
    17881 attttaatct ccaaaatcag ggaatgatgg caggatcgga acagctacaa cggtgcagat
    17941 caattaaatt gaagaatggc attaatgacg cgttcctgtt ccgccggagt catatctgaa
    18001 taaaacggca ggcgcaccaa tcggtcactg acatcctcgg tcacaggaca ggcgccttcc
    18061 tgtccgccaa actccttgcc catgaccgac aaatgaagcg gcagataatg gaatacagcc
    18121 tgtatgccct gctctttcag atgcgcaata aaagcctgcc tggtttccag cgaaggcatg
    18181 agcaaataaa acatgtggta ggtttgttcg cagtgttcag gcacacaggg aagcccgact
    18241 cccgatttct ccgcccagtc ccgaagccca tcatgatagc gtaaccagag ggctttacgc
    18301 ctgcgttgaa tactgtccag cgcttccagt tgtgcataaa ggaaagcggc ggtcaactcg
    18361 gaaggcagaa agctggagcc cacatcaacc cagctgtatt tgtccacctg cccccggaaa
    18421 aaacgactgc gatcggtgcc cttttcgcgg ataatttccg cacgttcagc cagagaagcg
    18481 tcattgatca gcaacgctcc cccttctccc gaagtaatgt tcttagtctc gtgaaaactc
    18541 tgggtggcaa gacttccgaa agtaccaaga taccgtcccc tgtattttcc atccagtccg
    18601 tgcgcattat cttcaatcag cggaagctga tagcgcctgg caagatccat aagcgcatcc
    18661 atttcacagc ccactccggc atagtgcacg gcaacaatgg cacgggtctt ttttgtaatc
    18721 agcgtttcca cctgtgtctc atccagattg agcgtatcag ggcggatatc ggcaaagaca
    18781 ggccgggcac cgtgaagcac aaaagcatta accgaggtaa caaaagaaaa agaaggggct
    18841 atcacctcat cgccgggagc gatacggcag agaatcgctg ccatttccag cgcatctgtg
    18901 caggaactgg taagaagcgc ttttttacac gcataacgtt cttccagaaa agcatggcac
    18961 cgtttagtaa atgccccatc ccctgacaga tgtccgcttt gcatagcctc ctgcatataa
    19021 gcgatttcac ggcctgtata tcccggacga ttaaaaggga taggcaaaga agatgaactc
    19081 attataatct cctgcggggc accctcatgc atcatcgggc acagcagcgt caacgtattt
    19141 gaaggagacg gcagacacaa cccgttcaga aaacatctgg tatctgctcc ttagtctgtg
    19201 tgtcttctgc tgcctttttc cggcgtaaac cccgtgagtc aataagaaac agaaatccaa
    19261 taaatcccaa tataacatta acaatatagt tatatacaga aagcgccgca gcagactccg
    19321 catcgatgcc gtaaaaagag agaaagtaaa taaccgaaag ttcgcggacg cctgccccgg
    19381 aaattgtgac gggaacagcc gtgataaccc atacaagcgc ctgcacaacg catatatcaa
    19441 aaaaagagac agagatgccg agataacgtg ccaccgtgta ttttgtgatg aaaacaacac
    19501 catgaatggg gagacacagc agaaaagcca taaggacccg aaaatagttt ttccgaaaat
    19561 aaagcaatgc accacgccag gtatccagca gcgaattaag tgctgatcct acttttccca
    19621 gtcgcaggaa aatcttttcc aacagtgcaa aaagccacgg ctgtgttgaa atcagactca
    19681 gtccggcaaa aagagccata agaacggcaa aataccggta aaaaaccatc cgatccggga
    19741 tacgggttgc aaagatgaga gatatgaacc ccagcatgat gatagagaaa agccctatat
    19801 agcggtcggc aacgatggca attaaattgc tggcgcggtt gctttttacc gtcttcaagg
    19861 tcatggctgt ctttacaaag tcgcctccga gagcgccggg cataatcaga ttaaacgcgt
    19921 gggcaatcat gagaaaacgg aagtactgcc aacgggtcag ctgccggctc gcatcgggat
    19981 tcagcagctg ccagcgcaat ccgttgagcc aggtccggaa aattgcgata acaaccagaa
    20041 ctgtaaagat ataccaggga atatcccgga tgtggctcag caccaactgc accttcacca
    20101 tgcgaagaag cacggcaata gaaagcagca atgcagtcag ccgaagccag aatactattt
    20161 tctttctctg atctgccgtc acaactcttt atcccgaatg aaatactgcg gcagctgctg
    20221 ctcttcccga agaatgcgga tcaaatactc cccgataact cccatggaaa aaaggagtac
    20281 gccaaaatgg aaaagattga ccataataag cgtagtccag ccgggcacag aaatgccgat
    20341 ggcaaaatag cggtacaaat aataaaaagc caatagaaaa ctcatggctg cgctgaaaaa
    20401 acccagcacg ctcaccactt ttaacggggc ggcagaggtg ctgacaatat tgttgaaaaa
    20461 atcccgcacc agcctgaaaa aagaataacc actgcggccg tagcgccgct cttcgtgagc
    20521 gacaggtaca ttgacaatat gagcggacaa atcaaccagc agccgaccaa ccctgggctt
    20581 atctatttgc aggcgcacca gttcatcaat aataaaacgg cggatcagcc gaaaactcgt
    20641 cagctccagt ttgggctcgc tgtgcagcgc aaggcgattg aacccttgca tggcacgggt
    20701 tcccagccgg cggataaaag aatgtttttt cgccacataa gaaccgatca ccacatcgca
    20761 attgctgtct ttctgcatgg catcgatcag tttaggaatc tcttcaggcg gatgctgcaa
    20821 atcatcatcc atggtcaaaa taaattcgcc ctgagcatgg gcaaaaccgc acatgagtgc
    20881 attatgctgt ccgaagtttc gtgccaggcg aataattttg atcctgttgt ctttgtgacg
    20941 caattcctgc atgacctgcc aggacgcatc cggagaagaa tcgtcgatga gtataatttc
    21001 cacagccaaa gacatctgtt caaaaacctg aaagacacgt ctgcagagca gccctagcgt
    21061 gggttcagag cgatagacag gaataactac tgaatagaga ggagtagacg ctgcattcat
    21121 aaataagacc ttatagtgct gtttgtgtcc ggcatagaca catccggcat ttcttcgtcc
    21181 gggcaaccct ttaaataacg gatcaggtac tccgtcgtaa aaggctgcaa gttacgtccg
    21241 gatgcacggg gctccttgcc ttctttttca ccatataaat ccaaaatacg gctgatggca
    21301 gtacaatcta tgtcaaaacg ttttgccagg gcttccagag gaacgagacc agtaggaata
    21361 tcttcatcca gatagcgtcg ctgaggtacg gatcgcttct gataggtcgg cgttctgctc
    21421 acagcattag cgagtgattc tccggggatc cgagccacac cataactgta atccaaccag
    21481 tgctgggctg atacgatggc attgtggata gtggaaaggc actcatggcg catttttcgt
    21541 aaagtctgga tgtctctttt ttctgtgcgt gactccgcca gacgcaggcg agccatcata
    21601 gcctgccatt cctcttccct gtcattgcca taaagtccaa agacaggcat attcaattta
    21661 tgggcgatac gcagtctgat ctgatcgatt tcctccagca attctcctac ttccggtcgg
    21721 gcagcaatcc cttccatata aaaagaatac ggttcgccct gccgttgccg tttctcgatg
    21781 tcatcgtaat tcagaagata gccgcccgga tgaaacacgg cgcctatatt gcccagcgaa
    21841 gttgtcgcgg ggacacggtt gaacagaacc tgcggaaaga tctccgaaat ctgttccctc
    21901 tgctcttcgc tcacctcgcc ttcgagcgac accatccagc ttcgttttct ccgtttgatg
    21961 taaacgccgt tctccaacgc tttggcagag taaggggaag tggaaaaaca gatcagcgga
    22021 tagttctcac ccacaatccg ccaaagatag ggggctgcca gtgttctggg cggcgtcaga
    22081 accagcggta tctgccgctg catcagtcct gcttctttca gcagagagac actctcctcc
    22141 aggtgctggg aagggtgaga cagaaaaatg aaatctgatc gggtaagcaa ttcatcaagg
    22201 ctgtgcccga cgctgctcat cccaagagga ataaagcgtg actcctcacc gatattgttg
    22261 ggagggcgtt cgagataaat accgccccgg gcattgaaac agctaacagc cgccctgccg
    22321 tgttccgtag cacgggcata gccgtagctc tgcagaccct ttgaaagaaa gtcggcgaaa
    22381 aaagcacgcc cggactgggt gcttacgccg tatatacctg gtttacaatt catttttctt
    22441 catcaaatgc tgtcggatgc aggacaaacc aaacaagtca gcggattttc atccgaattc
    22501 ctgaagcgaa agacattaca tgcgacaacg tctgttgata attttctccg gtagatgcaa
    22561 acagcaccac tctgtcaccg tcgcttttcg gtgattttac tgaagaaccg tcggcaagca
    22621 gttgtctgta aaagaaaacc cgacaagcat cgacggcttc tgcctcatat tcaactgatt
    22681 caagaatgcc atctttatca ggcaccagac tgtaggacgc atagtactga ttgtcatgct
    22741 gatccggaac gggcaaaggc tgctctccca cagagagttg aatggtagct ttataaaagt
    22801 cgtagttata ataacggggt accatttcgg ccatgagagg ccctccggca aggcggggcc
    22861 ccacgtcgat aatacgaggc acttcatttc tctcaagaat catgtcaata tgcacggccc
    22921 cccaatttat gccgaaagcc cggatagcag cttcattgac acggcggatc tgatcagcta
    22981 tttcagcgga aaaatttgcc ggaaatacaa cgccccgcgt caaattcata acctcttcat
    23041 ctatcagttt ttccggcatt accaaagtaa aaatttttcc atcatggacg aaagactcca
    23101 caccaagaac agttccgtct atgaattcct cgacaacaac agtgttcatc tttgaatagc
    23161 tgcgggcatg ctgaaaagca tcctgcatct gttggagcga cactttttcc agaaggctga
    23221 tccctttacc accgccgccg tcggcaggtt taaccacgac gggcagccgc agtgtctgca
    23281 ccatttccac tgctttctcc ggatcagacg cagagaaacc gggcgccata gattcgccga
    23341 tgatatcgcg gtatctgttt ttaaatacgc aattttccac tacctgaagc ggaggcactt
    23401 tatcactgaa tttaagcaga tgattgcatt gtgcaatggc atggattgcc gtgctggtgc
    23461 cgcaagtgac catccctgca ggctggtata tttttgccag gcgcagcact tcctctatgt
    23521 ctgtaaaatc aatgggatat actttgtcgg catatttttc acaaacgccg ccgcctgttc
    23581 cgaggcagac cgtcatgtaa cctgatgctt tcgctttttc tacaagcgga atctgattat
    23641 aagtggttcc gaatataaaa agtgtttttt tcatacagac cttattgctg aagagacagt
    23701 cgttctgctt ccttcagtat ttgaacgccc tcttcttcat atacatcaaa aaaaccatgc
    23761 cgggacgggc gtgttacaag acggggataa ggataatccg gatgtaccag accgggtgct
    23821 gccataaaac gatgttttac cgctgcttcc agtatgaacg ggtcagcaag catgcttgcg
    23881 atactctgaa gcgatccggg ctcataccgg ggatgaaggc gggcgatcgc atgaagcaga
    23941 tattccgctt ctttcagcag gtagtgcttt ctttccacta cttcattctg attacggtat
    24001 ttcattaaat cgggtgcgtg ttttttgaag agtctggcgg catgccgtac gatttgtgca
    24061 ctttcgacaa tatcctgtac cgttgcggcg tgacgtgcct cgcaatagct gaccagatgg
    24121 atcatatgcg gttccaatat catctgcagc aatgtagtac gagccagctg gtatttggct
    24181 tcagccggat ccggagaaaa gtgttcaatc cctgctctgg tttcacgcca ggcacgggca
    24241 ttccctttca gcctgccgac cagctccaaa gcagcgccca ttttggcaag atcggcataa
    24301 tcaccggttt ccgccggttt gttcaactga aactgaaaaa tcatatcggc aacaccacat
    24361 tgttccatca cggcggtgat caatccatag tcagcaacaa ccactgtatc atgtgcccat
    24421 cgggatgccc aatgattagg atcattcatc tcaacgggga tgccccgccg ggcaagatgt
    24481 gccaccgtct cctggtgctc caccagtgat tcatccagag taagcggacc acgcccgtcc
    24541 aattcattaa accagaaaag cggcactgcc tgatgagcgc cttgcagcat gccgactttc
    24601 aaacaatcat cggcaaaagc gcagagattg cgtacatgag catagggctt tatacccgga
    24661 taattgccac gacgggtcgc ctgataaagt accttgaggt cgtccacagt tttataaggc
    24721 actccaccgt cgttttttct tcctttaaaa gcttcaggct caccgaaata acgctgcgaa
    24781 agatcggaag agcccagaga aatttcgtct acagcaccgg cattggcaag tgcagcaatc
    24841 ccctccaccg tcggctgaat agagtcttcg ggaaccccga aatgggttcg cagacagggc
    24901 agttctgatt cttcaaatcg tcgaatcagg tccttgactg cttcggcgga cggtttttgc
    24961 agccccggct cagagcgcca cttatcgcct gcaatcacct ctttcgccat ctggtccagc
    25021 atttctatac caggggggca gagttcttct ttcaacagag ccagaattct gtcgcgccgg
    25081 aaatcacgaa cctcaaaata atcgagcacc tgctctgctc tgtcaagagg cggcaattcc
    25141 tgcgaaaaga attccacggg caccggaaac tgattgctgc gggcacggag tctggcgatg
    25201 ctttcaggaa gggcggcaaa ggcaacacgg caccaggtgc cgtcaccacg tcggaggagt
    25261 ccgttttctt caagagtatg gatgcattcg gtaaaaacag cctctgcttt atcaggtgaa
    25321 agccggtagc tcagtcctat atggctgggg cgatattccc gaacagcacc cagcaaaaga
    25381 gataatgtgt ctgctggcgc cgttatatgc ccggaaatgc atgccaggtt cgcaatacgc
    25441 tgtgctttga aaagcccttc tgtatgggga tcgttcccaa gagtcgcccc aataaaatac
    25501 aatgacatcc gccttctctc cgtcaaggca tattgaacca gctcccttga gttggctgtc
    25561 tgccgctact accgattgta aaagggtatt ctacctgaaa atcgggggca aagtcgcccg
    25621 ctctgcacgg catgtcatgt ctgtgggtgc aaccaggcaa tatttttagc gcttttggca
    25681 gcgcagggga aagtttttag tcttcgggaa cttcgacgcc tctgccggaa aatatcatgt
    25741 tatacgcctg gtcgcgagcg gaataattaa ccacataaaa tccgggcgta ttcaaccagg
    25801 tggtctccgt taagcccgct tcttctagtg aacgcaaaac aggttcgccg ggatcatctt
    25861 cattgacttc atccggaaag taacgataga tatcaatcac aacattgtct gccgaaaggc
    25921 tgccctcgca ctgatcttcc aggctaagga tgcgaatatc ataagccgcc gcgacatcgt
    25981 cccagtggtg atactgacct ttacgacaca catacaccgg gattgtttcg ttcagaaggt
    26041 aatcaggatt atctttaact gtcaagacag ggggcaatgt gtcgttcacc gcaataaaac
    26101 gaatcccttc cagtacgccc ggctcttccg tatacggctg ccggtactca tatcggtaag
    26161 taacacgata gggagagtga tcggcgagca gagggatctc cggatcggcg ggaatccagc
    26221 tttctccgga ttgataggca atctctgccg ctccgatcca ggcaatgggg tcaaactcct
    26281 ccccgacacg gactgatgcc tccggatcat tccagtagac tccacattca aagctattaa
    26341 atagggatgc gtccggatct atatacgtgg gctttaaaaa cagagaaact ttaagtacag
    26401 attgaaaatc acaggtgcgc ttaccgggat cagggaattg atcataaaca gtatcaacgg
    26461 gcaccacgtt ccagtcatat tgtatttcag gcgctgggaa gcccgctcct gtaatctgtg
    26521 cgattgcttc actcattggc aatcctatga catcaggcat gaaacagggc ccttcagaaa
    26581 caaccagatg aataaccccg ccgcagagaa agttgtctgt ttcaataaaa agcactgtgc
    26641 ctgcttctgc ttcggaggga acagcagtga cagaaccgga aatatatccg gcggtgcgca
    26701 gagcttcctg agcatcttca agagacatgc cagtcactgt cggaagcagg catggtccga
    26761 gcgaaactac aatctgtact tcggaattgc agagtgcaaa aatatccggt gccggactct
    26821 gagaaataac ctggtctgca aaaatattat catcgtgctg ttgaattact actggcacca
    26881 gatgcgctgc aagaatacgc gcctcggctt cttcgcgtgt cagaccggcg tcaacgacaa
    26941 gaggaacccg ggtcgtatcc ataactttta tataccgcaa aaagttattg gtgacatagc
    27001 ggccttcgtt gccggcactg tcctgaactg tataatcgat gcggtatata ccaggtattt
    27061 gtgtaaattg atcaaggggc acaatgatct cattgccgaa ttcgtcatac tcaaggctgt
    27121 aatcgggctt cagccgacga acggtctttt caacgctgtc ccagtaatct gtcggtcctt
    27181 ccgaaccgca cgcatcgagc gctgtacccg aatcaatcat gctccagtcc gcattgcagt
    27241 caagaatatg gacatgaaat acgcgagggt aataagtcca atccgcctca aaatttctgt
    27301 aaccgtaggg cataagtgca accggatcca tttgcagcat ggcaggctca atgacagggg
    27361 ggcgggtatc ctgcacataa atgcggcgta cgacagagag attgactcct gatgctgttt
    27421 ccgtgctgta cgtgatcctg aattcacttt ccagagtgcc cgtattgttg tctatggatt
    27481 cgacaggaac tccgtctttt gtaataaccg atgcaacaac aagcggactg ccgtcttcat
    27541 cctgaccgtc cgcgcccgga tccgtatagg aaccgccgca ttccacgaat tcctcttccg
    27601 atccgaataa aaccagccgg gcaccggatt cttcaaacgg agcccagcac gccggaggct
    27661 ctgcggaagg gtttactgca tagagcccgc cagattgcca caggaagagg gcacgcatgg
    27721 caaaagcaag agggaggtct ctctgctgcc agccctccac ataatccata gcttctcctt
    27781 ctgtgatcct tttatcctca ttcgtatcgg cagggtgaaa ggaaagaggg gcagcagatg
    27841 cagttcgtat aaaccccgaa atcagtaaaa gcaaactgca cacacagacg ataaacagac
    27901 gatttttcag aaagtgtttg cggcaaaagc aggaaatttt catgggacct tccttgcgtt
    27961 aaagagtttg aactgtcttc atcacggtgc agcccgaatc ggcaagaggt tttgttgttt
    28021 ttcgattatc ttactttagt ttaacacaat aaagtgtgaa aatgttcgct aaaaaactgt
    28081 tacggacgaa aaagagagtt ggctgcccta tcatgatcag ttgatttact ctgaatggct
    28141 tccgtatact cacaaacatg attcagacgg gctgggattc cccgttctat taacaaaaaa
    28201 caggcttttt tatatgcatt gcaaagacat agtcacgatg agcaggcttt ttatgaggtg
    28261 tgtcgcagcc gcggcactga tactctgtct ttggcagctg aattgtatgg cggacgacgc
    28321 tccactgccc caggttctga atggagaact ggatcaatgg aataatggcc gtctcgcagg
    28381 ctggatctat gtaccctaca gtgccctgga gatacagaaa agccaggaaa tctacaacgg
    28441 gcaccccgtt atcaccatgc accatccgaa gctgggcttt ctcgagcagg tctttgccga
    28501 tggatcaacg ctgatgccgg gagatatact gtccgtgtct gtgtcactac gttctcagcc
    28561 gggggcgact gtagatcttt cttttatgct tctgtacggg aagggcgacg atctgcagcg
    28621 caaatataaa aaagagattt acaccggcgg cggagaatgg cagcggctgg aggtgctttg
    28681 ggtcgtttca gaagcagatc tggaaaatct cgaaaaagta gtcattcgct tatctgttgc
    28741 aaatgcggaa gctcctgtgt ccgtgagcca ttttcaggca tatgccgcca atgtgggggt
    28801 gtcggcaaac ccgatcccgc cgggatctgt agaggtggca tggatacgcc cgtaaccctg
    28861 atccgaaatc accgtcttct tctttggcgt tttgccctcc gcgggttgac tcaacgctat
    28921 aaaggaagtc ttttcggtct gttatggaca gtaattactc ccctttgcat gctgggcatc
    28981 tatactttta tgttcagtgt tgtttttcag gcgcgctggg gccacggcgt cggtgattct
    29041 aaaatggctt ttgccatcgt tctgctgagc ggcttaacga tttatcagat tttttctgaa
    29101 tcggtcaatg cctccattca ggcggtcagc aacaatccca actatgtcaa gaaagtcgtt
    29161 tttccgctgg aaattcttcc ttttgcgggg cacatcagtt ttctcctgcc caatcttgtg
    29221 tggtttctca tggtagtggc gggcacctgg ctttttatgg gtggtctttc gctgaaaatt
    29281 ctgctgcttc ctgccattct tattcctttg agttttttga ccatcggaat cagctggttc
    29341 tgcgcatccc tgggtgtttt tatccgggac ttaaaccatt ttattacgat cgtattgcgc
    29401 atgctgcttt ttttaacccc gatcttttac gatacagacc gcattcccca tccgtggaac
    29461 tacctgctcc tttgcaatcc tcttgcccat tttgttgacc agacgcgccg gatcctgatc
    29521 tataatcaat ggcctgactg gtatgttctt gcggggatct ctctgttttc gctggcagtt
    29581 tttcagctgg gctatctttg gtttatgaga acaaaaaagg gatttgcaga tgtcctctga
    29641 tccctgtgcc ctgaatctgc agaatgtgag caaggtgtat cacatttatg aggcgcccca
    29701 tcaccggctg ctccagatga tctacggtta ccgacgtcgt ttttttcggg aatttaaagc
    29761 actcgacgat atcagttttc aagtgggacg cggagaaagc gtcggtatta tcggcagaaa
    29821 cggagccggg aaaagcaccc tcctgcagat tatagcgggc actctttccg ccacctccgg
    29881 aaactgccaa acctcaggaa gaattacggc gctgctggaa ctgggcagcg gttttaatcc
    29941 tgaatttaca ggccatgaga atgtgctcct caatgctgcc atcctcggta tgagcgaaaa
    30001 ggaaatagcc gccaaatatg atgatattgt tgccttcgcc gacatcggaa attttctgga
    30061 gcagcctgtt aaaacctatt ccagcggcat gaaaatgcgc ctggcttttg cggtgcaggc
    30121 ttttatcgat ccggaaatac tgatcatcga tgaggctctt tctgtcggtg acggtgcctt
    30181 ccagatgaaa tgcatgcagc gcatgcgcga aatgatcgag cagaaaacaa cgatcctgct
    30241 ggtcagccac agtttacaga ccctgcgctc tttctgttcc cgggtggtct ggctcgatca
    30301 gggccggatt aaaatggacg gtgacacacg tgaggtaacc ggacggtata gtgaagtgct
    30361 tttcggaaca gcagtacaaa agcaaaaaga gaaggcagac ccggaacaga acattcgcga
    30421 ggcagggcca tccccggatt ttccggtaat aaacctggag acagcatccc gaacggaaac
    30481 actgcgaaga tggggcagcg gcgaggcgaa ggtgaccgct ttttcacttc atgatgacag
    30541 cgggaagatg tcaaaggtct acgaattcgg cgtcaccctc cagctcaata tagaggctga
    30601 agtaatcgat gaacttccgg atgcgttttt ttccatggct tttgctgtaa aaaaccggca
    30661 gggtcttgat atcatctcta tcagcacgga ccggctcggt attttcacag atccgccgga
    30721 tacaggaagt aaaatcaaag tgcgatttac ctttcctctg cagattgcac cgggagatta
    30781 ttcgctggta cttgccattg cctataccca tgaaggcaga cgcacctaca tggattacgt
    30841 tgaaaatgcc tggtattttt ctattctttc ggatcggccg cgctacggca tttatgagcc
    30901 tgatgcaaca gctgtttttt tagacacctg aatcttggga gaaacggatg gacacacagc
    30961 ccggtatcaa ctctccagag tattgggacc agcgctttca gcaggattgg tccgccaatg
    31021 gaggggaatc ctatacccgt gcctacggtg aactgctgaa agagcatctg ccgggatttt
    31081 tttttcagga aatgtgcaga gagcaaatga ctgtctgtga ttggggctgt gcagaaggcg
    31141 aagcggttgc gtatttaagt caactctatc ccgaactgga tatcagcggt gtggatgttt
    31201 ccgccaccgc tatcgataat gcccggaaaa aatacccatc gctcaaattt gctgcggaga
    31261 actggcttga tccgaaccgg gagctgcccg catacgatgt ggttttcacg tcccatgtcc
    31321 ttgaacattt tgtgaatccc ctggagatca tacagcagac actggcacgg atcgcccgga
    31381 caatgattat tattctggtg ccttatgcag aagatcttga cagaaaagat ccggagcact
    31441 ttttccgctt ttttgacagc aatctgccgg tccgctggcc aggctgggtc tgtactcatt
    31501 ttcaggtgct cgataccaga atgcgggcag gcttatgctg gcgcgggcag caggcgctcc
    31561 ttgtttatgc ggaagagaac tgggcaaaaa aagccaatcc agcccgatgc ctcaacagtt
    31621 ttcgcggaga ggccgctgcg cttctccctg aggtgaagtt gctggaacag gaaaaacagt
    31681 tgctggaaca ggaaaaacag ttgctggaac aggaaaaaca gttgctgcgt gcacaggaag
    31741 aacagttgct ggaacaggaa aaacagttgc tggaacagga aaaacagttg ctggaacagg
    31801 aaaaacagtt gctgcgtgca caggaagaac tggcgaagcg gaaactggaa atacagtacc
    31861 tccacgatgc caatctgatg cgtgttaacg atactttgcg ctcaaaaatc agcactattg
    31921 aagaaaacct gaaaaaagaa accgccgagc ataaaaacct gcaaaaaaaa tacgctttgc
    31981 tcagcaatga cgctgaaaca cttcaaaaag agctgaaggc acaaaaagca gaaagcgcac
    32041 agtttgcaag ccggaatgag cagctcagcc aggatctcgc ccgggcagaa tcaaattacc
    32101 ttgaaacaaa aatgatcctg cactcgcccg ggaaaattgc gctgtattat ttacgtgcat
    32161 tgaaaagaag gctcggggct ggatctgaaa gcccagcgca aaaaacacgc cctctttcat
    32221 cagatgcccc gcataccgac gaggagcaaa gcggcaggat tccttctgaa aaaacagctt
    32281 cgtcaacatc cccgaaagcc acagcacctg aagcaaaaga aattcgtgtc gcacccggag
    32341 cccgtcaaat ccgcaataaa gacaaagcgt tgaaaggtct tactgttgcc tgcattcttg
    32401 atacgttttc atatgcctgc tttgcgcctg aggcaaactt tatacctgta acgcctcatg
    32461 actggcggca gacatttcag caggaagaga ttgattttct gtttgttgaa tctgcctgga
    32521 acggcaatga cggtgcgtgg cagtatcgga tcgcctctta tgccaaccct gcaggaaacg
    32581 agctggatga tctgattcaa tggtgcaata cccaggatat cccgactgtt ttctggaata
    32641 aggaagaccc gccgaatttc gaccgcttta tccggactgc tgtcaagttt gattatattt
    32701 ttaccactga tgaaaattgt gtgccggcct atagggaaaa atgcggacac gatcgcgtgt
    32761 acccgctcat gtttgctgcg cagcctgcta tccacaatcc ggtcgatgcg aagtggggct
    32821 acaggagaaa tgtctgtttt gccggtactt actatgcgga ttgctttccg gagcggaggc
    32881 ggcagatgga aaacctcctg gcgggcagcg tcccttatgg tctggatatt tttgaccgta
    32941 tgcacggcta taccggagaa gaccggaacc gttatatatt tcccgagcag tttcagcctt
    33001 atatacgagg gcggcgcagc tacgaggaaa tgcttgaaga gtacaaaagg tatcgggttt
    33061 ttctcaacgt gaattcggta caggattccc cgaccatgtt cgcccgtcgt gtttttgagc
    33121 tgctcgcctg cggaacgccg gttgtttcca cacccagtct tggcattgaa aaaatcttcg
    33181 gtgaacttgt gcctcaagtt tctacggtcg aagaaaccag aaaagcactg gaacttcttg
    33241 tggaaagtga cgaggtctgg catcgccgtt ccattcaggg aatgcgtaaa gtgatggcgg
    33301 agcattgtta tgagcaccgc ctcgctgaaa ttgcagcaaa gctcggttcg ggatctgctc
    33361 cttttaaacc aacaattcat ctcatccttg aagtgggcgc aggattcccc gaacggatta
    33421 ttgaagagat cctgtcgcaa cattatcttc ccagccggat atttatgttt cagggaacac
    33481 cgggcgagac tttgcgcaag aaagcttttg agcgatggct ggaaaaaaca ggctgcaatg
    33541 cggaagaatt tccgtacagt ctgtgcagcg acgaagaagc gcatgaaaaa atccggaccg
    33601 ctcttaccgc cgccggtatc tctgttgcgt cttatgaaag aaaaaagaca attgaaaaat
    33661 tacagcagga caacagccag gattattttg cgctttggga tggaaaaagt ttttatggcc
    33721 cccatcatct gtcagattct gttcatgcgc ttcacttttc cgggacatcc tgtgtcggta
    33781 tttactattg cttccggatt aatggtatag gagaattttg cacaccgcct ccttcagttt
    33841 atgactggaa gaatagcaaa tactcaaaaa agaaccccca tgacgaagga ttcgttttcc
    33901 cggcatctat ggtgggcaag atggaaggtt tagaggaatt ttttgtggaa aggctccctg
    33961 tagatgagcc tgttggcgtc acggcggaca gtatcatccg ttatccttat gagtttgttt
    34021 attcttcacg aaacccggaa cctcttcccc atgaaattcg tgcccaggtg tttatctgat
    34081 ctttttaaat catctccgaa gaaaacaatc cggtcggagg ctgcccggcc tgtccgtttc
    34141 cttctggcgg gtcatgatct taaatttacg ggtccctttc acgactattt ctccaacaac
    34201 ccgcgctacg aatttcgcat tgatgagcac ccgggacacg agatcgcttc ggaaaaacag
    34261 tcccgggaaa gcctggaatg ggccgatgtc atcttctgcg agtgggcttt gggaaatctg
    34321 gtatggtatt ccaaacataa aaagcccggt cagatgctgc ttgcccggct tcacagccag
    34381 gaatggaact atcgggaccg gctggactat atttacaaaa cggactggaa cagagtcgat
    34441 aaactgctgc tggtcacccc tgtaatttat gatcacatca tacaggaatt tccacagctt
    34501 ggtcggggga aagcacaggt actgaactgc cctattgata cagatgccta cgttaaagca
    34561 gggcaaagtg ccagaaaaaa atatgccctg ggctttgtgg gcatggtgcc tgccatgaag
    34621 cgcctcgatc tggcattgga gatgctggaa atacttcagc gcgaagtacc cgagttttcg
    34681 ctgcatatca aaggaaaacg cccggaagag tacccctgga tgctgcagcg cgaagaagaa
    34741 atgcgctggt atgaaggatt gtattcaaaa ataaaagaat tgaaaaaccc ggacagcgtt
    34801 atcttttatg aacatggctc tgatatgcct gaatggtatg cctctgtaag ttttcttctg
    34861 tcaaccagtg atcatgaagg atgccatctg tccgtagccg aaagtatgct ggcaggctgt
    34921 gtgcccagca ttcgcagttg ggacggtgcc aaccgtattt atcctccccg tttcgtatgg
    34981 tcgacacctg aggaaggcgc ccaaaagata cttgctctgt tcgaagaaaa tgcccgggaa
    35041 gaggcttccg ctttcagccg gacttatgcg gaagagcgtt tttcccaagg gcacttatgc
    35101 gaacaggtgg acagcatgat catgcggcat tttgaaactg tctggcctgc cgttgtttcc
    35161 ggagaatgtc gggaaatcag cagccgtctg ttgccgaata tcctttttgt cggatatctg
    35221 gcttcgtcga gtcagagcgg ctaccgtatt cgcatcgaac aggaaatccg tgcgctggca
    35281 cgggcagggg cacggattca ttttgcctgc tgtcatcccg aaatgaactc agcggtgctg
    35341 aatgcattga aggtcgaatt ggacgccctg ccctgcccgg tctccttcca tagggcaccc
    35401 tctttttttg atatgaaact gactgctgct gaggtaaaag cacttctgga ctcgctggaa
    35461 gaagtgcttc agaaaaaacg tatcgacatc ctccattgtg aagccttgta ttgtgcgcgc
    35521 atcggcacca tgctgaaaga gcggaaaccg gaactgaagc ttttctttga ttgccatggt
    35581 atttcaccgg aagaggaggc catgggcggt gctgcaaaga cacgagttga gaaggtgaat
    35641 caactggaaa tggaagtgct tttccggaca gatctgaaca tctttgtgtc tgaagccatg
    35701 caacagcact tcagaaataa acacgcctat gtcggagaaa attactggat cctgccgtgc
    35761 tgcgtggcag aaagtcagtt cgccagcgac cgcgaggaaa ctgagcaaag actttctctc
    35821 ccccaaaatg ctctgatcct ttcctatgcc ggaacactgg cggcctggca gtgtggcgaa
    35881 gaaatgctcc gcctgttctc actgcttcat cagaaagaat cccggcttca ttttcttctg
    35941 ttgctccccg aaaaagaaca ggaaaaagcc cgggagtggc ttcatgacta tggcgtctcc
    36001 gatgagtcgg tgaccatgct ggaagcgcgc catgatgaag tgccgctgta cttgcaggag
    36061 gctcatgcgg gcttgttatt gcgcaaggaa gaccccgtga atcaagtagc ttcacccacg
    36121 aaattcggtg aatatctcgc cgcaggtctg cccgtattac tcacggaagg catcggcgat
    36181 ttcagcgcat tggtggaaaa agaagggctg ggcatgatcg tgccttcgtc tttgctcgac
    36241 acgaccgagg caaacgatga agagttggag cagatcttgc agtttctgaa acagaccgta
    36301 tcaggccggg accagatgcg acggcgttgc gtccactacg cccggacaga gctccactgg
    36361 gaaagacacg ctcatgactt tctaaaggct attacacgct gactctcaag cttttcagat
    36421 tccctgactc ttgtccagtc gttccctgta atgctcttca taataagctt tgaattcccc
    36481 gctcttgatt ttgcgccacc accattcatt ttcctgatac cacttgacgg tgaaggcaat
    36541 accttcgtca aaattcattt tcggctccca gccgagcgct tttaattttg tgctgtctat
    36601 ggaataacgc ctgtcatgcc cggtccgatc ttctatatac cggattaatg tatccgggcg
    36661 gttcaataaa gagagcactt ttctggtcag tgtaatattt tcccgttcat tttctcctgc
    36721 aatgttataa gcttctcccg gcgttccttt tcggaaagcg cagtcgatgc cggcacaatg
    36781 atcctccaca aaaagccagt cccgcacgtt ccttccatca ccatacagcg gaaggggctc
    36841 attgtctata gcgttgctga tgaaaagagg gagcactttt tccgggtact ggtatggacc
    36901 ataagtattg gagccgcggg ttatgatcac cggcatgcaa aaggattcaa aataggaaag
    36961 cccgatgagt tctccggagg ctttgcttgc agaataggga tttcgcggaa ggatcgggga
    37021 atcttctttg ctggagccat ccgtaacgct gccgtacact tcatccgtag agacaagcaa
    37081 aatccgttcg agattcattg cacgtgcctg tgacagaagc gtattgaccc ctcctacatt
    37141 cgttgcaatg aaatccgatg cgccgctcaa acttcgatcc acatggcttt ctgcggcaaa
    37201 atgtaccgcg ccgtcacagc cttccaatgc atggcggaca tgctccggat tattcacgtc
    37261 accccggatg aaggaaatgc gtgtttcatc aagcccttta aggttgtcca gatttccgga
    37321 ataggtcagt ttgtccagta caacaacatg ctgatcaggg tagttgcgta attgggttcg
    37381 gacaaagcag gatccgataa aaccggcacc gccggtgacg agaatttttt gcatagattt
    37441 tctcctgtgc agcactcgga actaaggcgc atcgacactg cggctttagc cgattttata
    37501 aaggcgcatt ttggcttagt ttttgttttt ggcgtttgac aagaaaacgc aggtaatctc
    37561 cataggcgct gcttcccatt tttttggcag atttttccac cgctttcaga tcaatccatc
    37621 catttttaaa ggcaatctct tccaggcagg caatctttaa gccttgtctt tcttcgatag
    37681 cctgcacaaa catcgtggca tcaagaagac tcctgttggt gccggtatcg agccaggcaa
    37741 cgccacgccc gaaaacagat acctgcagcc gctgctgttc cagataaatg cggtttaaat
    37801 cggtaatttc gagttcgccc cgcttgccgg gtttcagatt tttcgccagt gacacaacat
    37861 ccggaccgta aaagtacagt ccggggatgg caaaattact tttcgggtgc tccggttttt
    37921 cctcaaggct gactgctttg tttgaagtat caaattctac aacgccatag cgctccgggt
    37981 cgctcacata ataggcaaag attaatgcgc cctcctgaat ttttgccgct ttttgcagga
    38041 tagcagacat gttgtttcca taaaaaacat tgtcgccaag gaccaaagcc acaggttcac
    38101 cgctgcaaaa ctcttcaccg ataataaacg catgagccag gccatgcggt tgaggctgtt
    38161 cggcataaga caactgtata ccgaattgag aaccgtcgcc caaaagcttc ttgaagagcg
    38221 gaagatcgtg aggcgtagaa ataatcagga tgtcacggat gcccgccaaa aagagcatgc
    38281 ttaatggata gtaaatcatt ggtttgtcgt aaacagggag caactgttta ctgactgcca
    38341 cagtaatcgg gtgaagccgg gttccggcgc cacctgccag tacgatacct ttcatcttaa
    38401 aatattcctt tgagtacttt actttttttt cgattataaa ggtcgtgcct gaacatatca
    38461 agccaatgcg cttacttcgg attgctcctc gttttccaat gctcaggttt aagt
//
LOCUS       DAOPWB010000010        38273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1011_length_38273_cov_5.261081, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000010 DAOPWB010000000
VERSION     DAOPWB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 38273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38273
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1011_length_38273_cov_5.261081"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKN92_02045"
     CDS             <1..302
                     /locus_tag="PKN92_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ47605.1"
                     /translation="VVEGNHHVFHLYPVMLSPEQSGISKEDFVYRMLYEKGVKVGTHY
                     IPLHWTTAFQNRGFRRGQFPVADRVGENLVTLPINPRQTREALDYLIESIRSLIG"
     gene            413..1729
                     /gene="xylA"
                     /locus_tag="PKN92_02050"
     CDS             413..1729
                     /gene="xylA"
                     /locus_tag="PKN92_02050"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNZ47606.1"
                     /translation="MKVYFPEVAKPIAYEGPDSKNPLSFKHYNPKEKVLGKTMEDHLR
                     FAVCYWHTFRSGGADMFGSPVMDREWLKGDSVIEVAENTLQAAFEFFTKLGVKFWAFH
                     DDDLIPETGSLAESNKQLDHMVKLAKKMQKDTGIKLIWGTCNLFSHPRFMSGAATNPN
                     PDVFAYAAAKVKKAIENTKYLGGLGYVFWGGREGYETLLNTDMGREMDHLGIFLNMAV
                     DYAKKIGFTGQFYIEPKPKEPTKHQYDFDAASCYAFLQRYGLDKTFKLNLEANHATLA
                     GHTLIHELEYAGANGILGSVDANRGDMLLGWDTDQFPTDLYDTTQIMYTLLKFGGFKT
                     GGFNFDAHVRRQSIDAVDLFHAHVGSMDCFARGLKIAAQMHKDKGFANFVKNRYSGWD
                     KGIGKDIENGKVGFEELEAYALKKGNPKIVSGRQELLENMLNDYIK"
     gene            complement(1789..3060)
                     /locus_tag="PKN92_02055"
     CDS             complement(1789..3060)
                     /locus_tag="PKN92_02055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ47607.1"
                     /translation="MSHCADRNPASPSDWNRWGMTPPQRVALVAFLFSGAVNSALLVM
                     PFFLFNQMNQGATLSGIFIGLASLGYTLASLFSSRFVARARHGLNWSLFGVTGFLIFL
                     GAMPAFRTPFLCGFFFVAAFTFSAFAWPAYHSWVGAEPFPEARARHMSLVNVAWSTGG
                     AAGPLLAGPFYEIDFRFPYLFIAGLCLLSLVLIASVPPEKSYFVCLSSDSTQSRRAHD
                     RASESFLYSAWCATFAAHITVGSTRAVFPKQLDELISTGQLRYLWESHASAWLNSASA
                     SRFSWLAVIMGLATGLTFFIMGRSAWWHHRFSMLVVIQLFTGAAMWSLGYTHSIIVML
                     IAFSAIGMNLGVAFFSSVFYGMANPTTKHGRAAINEGVVGLGGMTGSLGFAAVGIYAG
                     LNLPFRLMPLVMIAIILLQWCLIRWRRSGEA"
     gene            complement(3176..4201)
                     /gene="ruvB"
                     /locus_tag="PKN92_02060"
     CDS             complement(3176..4201)
                     /gene="ruvB"
                     /locus_tag="PKN92_02060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNZ47608.1"
                     /translation="MSRDEVIIPGRVEDDASYDDQIRPKRLDDFPGQEPIKDKLRIAI
                     EAAKQRSEPLDHLLLSGPPGLGKTTLARIIANEMGVDIKQSSGPVIERQADLSAILTS
                     LENFDVLFIDEIHRLNHAVEETLYSAMEDFEVDIMLGKGPTARSMKIGLRPFTLIGAT
                     TRAGLLTPPLRARFGDTCRFEMYTPEELERIITRSARILDVPVQPEGALEIAARSRGT
                     ARIANRLLRRVRDFAQVKGDGIITRELAEGALELLRIDALGLDDMDRQILEVLIDKFG
                     GGPVGLASLAVAVGEERQTLEEVHEPYLIQIGFLKRTPQGRMATPHAFRHLKKSPPPA
                     GGQGTLL"
     gene            complement(4188..4805)
                     /gene="ruvA"
                     /locus_tag="PKN92_02065"
     CDS             complement(4188..4805)
                     /gene="ruvA"
                     /locus_tag="PKN92_02065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNZ47609.1"
                     /translation="MFAFLRGEVVASSVNRIALDVNGVGFDVLVPDSAQRKLPVGQHA
                     TLLTYCHIREDIFQIYGFLTEEDRALFIMCMSITGIGPKVALSILSTLSVSAFARAVQ
                     EHDITAMSRVPGVGKKTAQRIILEMKTRMGQDPELNALLGDPQSAEETGDDDVYEALI
                     SLGCTAQEAKKASAHARKMLGSEAPDEELVREALRSLAKAGHVQR"
     gene            complement(4885..6177)
                     /locus_tag="PKN92_02070"
     CDS             complement(4885..6177)
                     /locus_tag="PKN92_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47610.1"
                     /translation="MSKNKDIVGVIDFGSRAIRVIIGSRLRNGQTHILGYSSVPTQDA
                     VHFGAIQDINLAGKLANQALTQAQAMAKEKTYYLYCGIQGSTEMSGIHDGKVEIENGI
                     IKPEHLEAARHNASLECVTPESRPVSCIKSEQWTVDDIRVADPIGMQGHVLKGRIHFT
                     RLSIFLENNLRTCLENQKKKIADFVYMPLAAAHGCLTLEDIRLGAAVINFGSTSTSIT
                     VYNDNRLIATEVFKWGSGHYVGDIAAVLNVYFEEAVELLLDYGINIDLFETPTKGGFK
                     LSSTSIAPPDSDVTIKLHKTVTGAPGTVAKSKLDAVIFERSKQFAALIEDYLKTNNYL
                     MKLSRGLIITGGGAAVSNQDKILEKTIGISTRIGLPLGFNELPDAINTPEWAPTIGIL
                     NYAFAHRGGTGTASAKNGRQTKNSFLTPIKWFFNDFIW"
     gene            complement(6236..8152)
                     /locus_tag="PKN92_02075"
     CDS             complement(6236..8152)
                     /locus_tag="PKN92_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47611.1"
                     /translation="MFNRARNHDNDAATQTENEPPVSSQAQEEEEESMNVYPDSTVPP
                     SDSENTAKTDPSQLVAALAPFQRCLEDAYHQNDAELWYDNCREALLNAYLICKQNQWV
                     PVRNTIQEMGRILHSYHAAGETFECLSFLRESYQALTVMVGDIIVGKVRDGVIRKWAA
                     LYEKTVSSMTAAGIPLTRDTEEKEIVTAHDAPEEEDPEQESFEETEAEEEVPCEETED
                     GDLEPSEETSGEEPEEKVSDSQTSDAPDQTCFPEINAAEMDGKADNAPAVKHQTQPEV
                     LSINLPPLMDDFFDTPPSAASAPEDTLILFPEQQKSPEDISFSQTDEIYEEENTHEEE
                     EYPDPENNEELLEDEIQEEVTEDLFSLVIEEDEGQEDFFAEDDEGSAQAQESLSATEE
                     EALESASPSEKDTPPESRSSHPLQPEEDSHQILEHIQASITREDIDHARAGALQLALS
                     LDKKAYETAKSAVQQAEQHLEENARAIQEVEEKTIMAGEALARMQDELECREEERNHA
                     REVIAQIDIEVGDTLTDIEELSRQISELEKKRALKNEELAKIYERKEGALAEESRIST
                     DAEALAQKVAAALTHVDDLHDDKKRYETERKLIENEITDARTVMERHHASLLQMEAML
                     ANALNYIRSDEDAR"
     gene            complement(8366..9967)
                     /locus_tag="PKN92_02080"
     CDS             complement(8366..9967)
                     /locus_tag="PKN92_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HNZ47612.1"
                     /translation="MYQRSLLWGIALFILVLALGAAFRLPQLDRRPLHGDEANQAVKT
                     GALYDRGFYEYDPFEHHGPTLYYLELPFLWLSGAKDFTDSTIVPYRLLTALGGLTLIA
                     LLWYLRDLMGTAAVLWAALLMALSPVMVYYSRYYVQEILLVCFIQAAFSFGWLYFKKP
                     SAFRALLFGVALGLIHATKETCILMVFSALAGLAVTLWLYKSSPSRSFKEMAASFFTR
                     QRKLHIVLASAAAVTVSVVFFTSFLTHARGPLDSILTYTHYLHRAEGAGSSGIHDKPW
                     YYYLQLLSYTHRQTGPYWTEGLVLFLAFIAALMILYRTVFPGKDNKEKTAQTRFQPFL
                     LVYALTLLALFSAIPYKTPWNILPAFHGLLLLAGIGAARLTAARFPLLVRIPVTALLL
                     AGAAHLGWQSYQGNFVYDADTRNPYVYAHPSRAILKLADRVEQIAAVSSEGKNMRINL
                     VQPIGDYWPLPWYLKSFTRVGWWTALPEDLDAAMILAPQALHDAVEARLKGNYFVEFQ
                     ALRPGVLFHVWIQRDLWDRFMETRR"
     gene            10194..12008
                     /locus_tag="PKN92_02085"
     CDS             10194..12008
                     /locus_tag="PKN92_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HNZ47613.1"
                     /translation="MNILGISAFYHDSAAALLCDGEIVAAAQEERFSRKKHDPCFPKH
                     AAAYCLEEGGLDISQIDYVAFYDKPLVKFERLLMSYLALAPKGYLSFLSQMPSWLKEK
                     LFVPSIIRKELGYDGKILYSTHHLSHAAAAFYPSPFEDSAIITVDGVGEWATTTYGIG
                     EGADIRILKQINFPHSLGLLYSAFTYFCGFKVNSGEYKLMGLAPYGEPRYVDIIKEKL
                     IEIRDDGSFRMDMRYFDYCAGLRMTNREFAELFGGPERTPESRISQREMDLARSVQAV
                     TEEILLKMAAHVHQETGKKNLVLAGGVALNCVANGRILRESPFASLWIQPCAGDGGNA
                     LGAALFAWHQLLRKERVPDARRQKATYLGPSFSDDAIQQFLDARKVPYTRLAPEEVPA
                     KVAELLASEKVVGWHQGRMEFGPRALGGRSILGDPRSREMQSVLNLKIKFRESFRPFA
                     PTALADHADDYFDLRGHESPYMLLVADVLESRLRHLSEEEQQVAGFDKLRVIRSELPA
                     ITHVDNSARVQTVRREDHPLYYDMIRAFYERTGCPVVINTSFNVRGEPIVCTPEESYT
                     CFMRTRMDYLCLGSFLLNKEEQEAWEEEGDWREEFELD"
     gene            12026..12436
                     /locus_tag="PKN92_02090"
     CDS             12026..12436
                     /locus_tag="PKN92_02090"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNZ47614.1"
                     /translation="MLDIDTSDRKEQRKFGFVMAAAIVILGGIRFALHGFTAIPWAFL
                     AVAVLFAVPAALFPGALRPVFVVWIKFALVLNWIVTHVMLTLVYYLIIVPMGLIMRVA
                     GKDPLNRKWLPATESYWEEPEEQPEEFERYHHQF"
     gene            complement(12475..13620)
                     /locus_tag="PKN92_02095"
     CDS             complement(12475..13620)
                     /locus_tag="PKN92_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47615.1"
                     /translation="MLHKPLGIIMNGVTGRMGTNQHLIRSLMEIRKEGGLRAGEYCIM
                     PEPFLVGRNEDKLKALSEACGGLPWSTDLDAALSDNKYSVYFDALSTVLRVEATRKAI
                     RAGKHVYCEKPIATNSDEALALYHAAEEAGVKHGVVQDKLWLPGLLKLKRLIDTGFFG
                     RILSVRGEFGYWVFEGDAVPMQRPSWNYRKAEGGGIILDMLCHWRYVIDNLFGNINSV
                     SCLGEIHVRRRWDESGQIFEVDTDDAAYATFVTEPGIVCQFNSSWATRVRRDDLLTLH
                     VDGEKGSAVAGLTQCHIQPAAATPRYVWNPDLGRTLEYASHWTCMPDTENYKNAFRAQ
                     WELFLKHVVSDTPFRWNLLEGAKGVQLAELGMESWQRRTWLDVPDLV"
     gene            complement(13734..15047)
                     /locus_tag="PKN92_02100"
     CDS             complement(13734..15047)
                     /locus_tag="PKN92_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47616.1"
                     /translation="MKKQHLSRRNFLGTACAALSAPLIVPARALGREGAVAPSERIAM
                     GFIGLGGQGSGHLFGGSWTYVAGGYLGRREVQVQAVCDVWKQRREDAADRVNRHYAHT
                     TGQTSYDGCRAYLDFRELLAREDIDAVLMALPFHWHAPMAILAARAGKDVYSEKPVAL
                     TIEEGRLLSNTMRRYARVYQAGTQQRSEAEYGGKFQRAWELIRNGYIGELREIYSFSP
                     GGGFSANMQNTRLKEQPLPEGFDWNLYLGTAPWQPFTGGNVHCGIFAYGDPNWGPHHF
                     DVVQWVLEHQFNGPEAIDYEEDHAVLLYPGGLKVHCCAHPEIEVGATGGACFIGTEGT
                     LAVDREGIMASPRNIMDISIRPQEIHPYKSPIHAGNFLDCIRTRKRTICDAETAHRSM
                     SLVLLAGIATQIKGYARWDARQECFPEHEDANRFLAYARRPEWNI"
     gene            complement(15044..18634)
                     /locus_tag="PKN92_02105"
     CDS             complement(15044..18634)
                     /locus_tag="PKN92_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ47617.1"
                     /translation="MIFYLRMHCFLALALSVVFFPCEGNSEACTLSFGELGEGNPRAF
                     FQQVMPGTATLGGVGFQLTEQMLRVRAGSEKTLHFPATTAESIHFLHFTENAGDRVGS
                     YTLLYEDGNREEIPLQNGISIQDWWLTGPLPFAAEAFTDTLGGIHTGKSVSFWRFSVR
                     NPRPDVPLCGITATVIDPTVTLNILAVTLDHDRSERIAGIPVWVPGMDEESFILALLE
                     QPEKTGEEAKFCARLAQTGSRKCIPLLASYLLDEETSQGARIALEALPYEEARNALRS
                     GLAKSKGAVKAGILESLGKSGQKTDLPLLLSALDDKDPSVVRAAVLALGKFGGPPALR
                     SLWHTADKASEQCLPLVQEALLACTDTLPASGRKEAFRSYRQYLSRWSDSPGAAAAWK
                     GMLLTGGRNSADLLFQALMSDSDASWQGALSALPHMNSKKAVRRAGKALDQLQDSRRC
                     ALIAVLGQGKNMSALPFVLPWIEAEDQQTALDALDALIQLDSSDAVPALIQRAVRKDG
                     AIAEAALQALASLRASGTAAALVRYLDHADPAVTETVAKVLELRREDSVAPELRRLVH
                     HRTAKVRIAAAGALAAVGNASDAALLADVWHQSDSPEDAAACFSTLQKLVARLGSDET
                     YIQTLAACHARSNTPQRCALFSLFGTVGAPQLLALLGDAAATGTPEEKEAALKALSRT
                     RNPDALPLLQALLKSGISDTMRLPLIRNLAALSQADEMNDAARTALLRECLDGAKNLQ
                     EKRILLSALNRCSSPDALVLAEAWFHREGTEAEGAVAWGTIALALLDTNRTEISQAFP
                     ALFAEAQAAGLSANALEPLYRVRKILNAEAVPGKSLRFKEICIDTAFRSEGIAIADIN
                     RDGLPDVVVGDYWYAAPGWTAHEIRPPGTYDALTEYSRAFAVFAEDVNRDGWADVIVV
                     GFPGAPVYWYRNPGKTEGHWQEYLIAVEACGETVLYEDITGDGKRELIFAMNKRITWL
                     TPGQDVTAPWIAYPFTHQLEQFALFGHGLGIGDVNGDGRTDVLSTVCWWEGPTETTRP
                     DWTPYPADLGPDCAHMLVYDANNDGLMDIITSSAHDYGIWWFEQQPSGQALPSFIRHE
                     IDRSISATHALIQADLNNDGLADLITGKRFYAHGKEDSGALEPALLCWYELQRDMQGN
                     PSWTRHVIHEDSGVGTQFEVCDVDGDGLDDIAVSSKKGVRIFLQRRDSSS"
     gene            18818..19810
                     /locus_tag="PKN92_02110"
     CDS             18818..19810
                     /locus_tag="PKN92_02110"
                     /inference="COORDINATES: protein motif:HMM:NF022784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HNZ47618.1"
                     /translation="MLICFLAVGSLLQGTSIASDIDLPQMLYVWQRLWTDEVKSAVRE
                     ASGSAEGLCVLCGEIAGRGEGWRFHRVNVDWASLAPVKGTLWLVFRIHTGALESEHWD
                     ASTFAGHVQKTLNDGADAGCVIAGIQIDYDCPTEKLTLYQNWLQVLRGKLAGIPVSIT
                     GLPDWLTSRRLPELLAGLDHFVLQVHSLELDRESGRQPVLFDETKMMRWVGQMNSLML
                     PFRVALPTYGWRIAYDRYGQPQRLEAEGIHEAVAGWTYQDVSADPAVLAGAVRKLAAV
                     SGGNLKGIIWFRMSLPSERMNWSLKTLRQVMKGGAPQAHFEAELRLSDNHLVEL"
     gene            19850..20062
                     /locus_tag="PKN92_02115"
     CDS             19850..20062
                     /locus_tag="PKN92_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47619.1"
                     /translation="MRLHLQSGSILASDLMRGFRQLKNTEERYITVSGPVYSDGEKHM
                     AGWWLLSGDSDSEVPALSVEEIGLCP"
     gene            20053..22332
                     /locus_tag="PKN92_02120"
     CDS             20053..22332
                     /locus_tag="PKN92_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47620.1"
                     /translation="MSLKKFCFLSVLMTASFPGMVFPCAWEPMSSYFGEGSDKDLYYL
                     PDTHFDSEYLRITDQLLDLTVEEAYRGAQLSFYMDLPNYLTRMAVAQQRAETSEAEAL
                     ADALAENESKESFLEAYRFLRDLVNRYAQRQLDLRIHGEQGWTFQEEEHPEMLEQRQL
                     QALLDVCPDEIPPQFRLYLQGAGAFHSGDYRRAQSSFSQILQLPEAERTHKTVWALFM
                     LGRCAMELKALDEAESFFHQTQHAVDDGFADPLNLHGECFGWFGKIYLERGAFVEAVH
                     AYVEYRYQPEKWEMGKESLFILFQRIFSQETDLRALAQDDLSRQLITAWFTSQSRPNP
                     DRVKRWLESVALLPRQEELPGAGQLAWLAYHHGDMEAAARWQKQSDEHSLAGRFVYAK
                     LLLREGKLEEADALLKALVEDTNRDRLLLFEGHYPRVTGASVRAAEAAVLLKQDDYIG
                     ALKNFAPVFLEDAQFLARRILTLDELDRAMADLKSMDPQEVADTLDTGDYFNETEYQE
                     ALSGFLCALETVHAQRLARLNDWSDAAQHFQQAEALLKEEGDPEDAEDLSAAAQQIAD
                     LMRRTKESALSDREKAQLLMDAGALIRQYGSDLLGEWWLFQEPGAESLTEGGRDLLSD
                     DGLKRMDASRNVCGRQYSFRFIASDLMKEAAELLPDNDPLTAKALFLGGTYLKARYPK
                     EADFFYKALIRRNPNLKIAKQADELKWFPENFTDEILYTPRPPEGLRKRDVAILVAKG
                     ALIVLAVGLLLWGLKRRCD"
     gene            complement(22395..22946)
                     /locus_tag="PKN92_02125"
     CDS             complement(22395..22946)
                     /locus_tag="PKN92_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNZ47621.1"
                     /translation="MKATRELIQEHEGITLMLKILDAVVKKAQQGTQVPQEDLDGIVE
                     FLTVFVDKCHHGKEEDFLFPELEKLGIPKENGPIGVMLSEHEEGRGLIADLKAAVAAY
                     KGGDSAAVAAMSKAAEGYIFLLNAHINKENNVLFAMADNHLNAEKDDWLKAQFDALEL
                     ERIGAGKHEAFHAMIHRLEKAYL"
     gene            23261..24316
                     /locus_tag="PKN92_02130"
     CDS             23261..24316
                     /locus_tag="PKN92_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNZ47622.1"
                     /translation="MNDTRNACCPSGNSSDLPVLQFVLRNYHNFNARSTRDALIAYWR
                     LLEQGGKMFWAVAGAMSSAQLGISIAPAIRAGLIHGMSLTGANLEESLFRLVAHKHYK
                     DIPEYRYLRKEDDTRLLNERLRRVTDTTIPEDEAFRAVEQIILPMWKEATASGTRRFW
                     HEYFYEVIQAIPSSLYEGDPEECWLLAAAKANLPIVVPGHEDSTFGNIFASYVQTGEC
                     NASIVKSGIEYMTSLYSIYPKLSEGAGVGFFQIGGGIAGDFPICVVPSIIYDLEKEAK
                     PWSYFCQISDSTTSYGSYSGATPNEKITWDKLTEETPMFVIESDATIVAPLILSALME
                     CKANPEAANALIARYGD"
     gene            24426..28514
                     /locus_tag="PKN92_02135"
     CDS             24426..28514
                     /locus_tag="PKN92_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47623.1"
                     /translation="MPIQRVLKSFLFLTLSSSFWFAYPVCSETRPFLDAQRHSFVLSF
                     SDAEKKETLTAGCRTKLFNESHYNILLAHLQKSETGHWELFTTPGDGILHLYAPGRVP
                     DHVHTEKSITDGQWHEIIAVLDGSGVRLIVDGEVAAEAEAPARTDSFLSGDLVLGALV
                     EGTFGCEGLITDAFLLSGASGHEGQLFLNDRFTEEAFAYWRFSSEDPLCGEGSEKRDG
                     VLRPAGVVRVNPSRQIPGGMSAALQELPPLEDTAPLRHSLAQAVQSLDLAFLGEPDVS
                     DGVFRQWSHDYDWMGKKEYPESRPGGPGEEKLRREVFDPEALPVPGESPLQAVLRRTR
                     ALLDLMGSTVDLAEEENAWNRLHQIAEKVAKQSDSQGKKGLYLAACALRRRIMLKNPL
                     LDFDEILCVARGTFEGSVRSNPTTLDVQGGHFVTQYFGFNALPGGGLYLIRNFKGKPE
                     IVDLVQHAVVEEGAIKGQNLEHGAFATPELSWDGETIFFAWTANPDHQWIYSKERCFH
                     LFRINKDGSGLVQLTDGAYNDFDPCQMPDGRIAFVSERRGGYIRCFDAYLKVRSYTLF
                     SMNARGEEMHPLSYFETSEWNPSVNNEGQLVFTRWDYVDRENCLGTRFWISGPDGSDP
                     RAPHGNYPRPYHTFPDHEPWKGETGHERDSRFGAPLVEMGIRAIPDSSRYIFTAAPHH
                     GSVYGTLCTLDLRHEDDGHMSQIRRITPEESFPETEMPGRRHYRYGTPWPLSEDLYLC
                     NEWENLVLLDRFGNKELLCDLPSLPCAIDERLRIIDPVPVRPRPVPATRPVLPAKEQG
                     DPATIAVMDVYNSDLPFPENTEIKWLRVVQNIPKPNHAMGEPMVGYERENTPRVPLGI
                     VPVEADGSAYFEAPVAKQLIFQALDENFMAVQSMRSSAFVHPGEQITCLGCHESPHRA
                     ANRTTSPLAMRRAPSRLEPECGQVEPISYYRQIRPIFENRCVPCHIKEDAEPKDMSYE
                     ALKEEYTFWFSGAMFGDMTTAYSGVHGGSRTIPGRFGARASKIGQALLDAVHCDVVSE
                     EDRRRIIQWLDCNSLRLAAFSREEEQVAGALVWPELDVDPENPTGVEGRGRPLRHHFW
                     HENLWGPFPVLITDAEKECIRLLDAQNNLQWEQALADPVSAHMLPDGHILAASRQGVQ
                     ELTRQHEQCWQFTVDSGDEVVSCQPLPDDRILLGVAGENRLMLLNRQGVVEKTVMLPP
                     AESRDVRLRCCTVVQENLYLVSFDGEKRICGLSDTGQCRLDLLCEGAPVALVGLKNGH
                     FLVLTEQTICEYDRQGHYIWEALGPDFADIKIRAFSSVCVLENGDLLITNQGADRAEG
                     VSLFEMTRDHRVVWQLNRRDAGDAVHCQALFDDNLWMPRPAL"
     gene            complement(28518..29804)
                     /locus_tag="PKN92_02140"
     CDS             complement(28518..29804)
                     /locus_tag="PKN92_02140"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM143 family protein"
                     /protein_id="HNZ47624.1"
                     /translation="MLFFRQKDDPALKQAALQAEEDLFFVPYSRRDIIDMCLDQGKLA
                     GQEKRFRQLATQLTHLIHFEFHQKIEALKSAYAPMNADFRPSPFETGEEAGHVREFYS
                     LFQHLLEKANYKAIPEEDIQRAFSEDALFTIRLQVDFNSFSEYHFFYRGATVKEECLP
                     VFMGLRSKSISFINYDRVVMYIRWKKTLDSTQKINPEAAGGTALLKLFQNVPRPDLEM
                     LFPNSRVGMRIMDKLLFGIPAAVTGSIMVLTRLSTTLFVLGSLLAFWLGLSSQPVEWN
                     KTTMAVIFAGLLTLGTYLWRQFNAFKNRKLRFLQTLIRHLYFKNLDNNIGVIHRIADD
                     AEEEECKEALLGYYFLLTAERPLTVQETAKKIEDWFLNRWGCVVNFDIDDAADKVLRL
                     GIVRKENGQLAALSLEEALSLLQTQWENLTTLNGLG"
     gene            29837..30109
                     /locus_tag="PKN92_02145"
     CDS             29837..30109
                     /locus_tag="PKN92_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47625.1"
                     /translation="MHSNRASPPDKKTSGKLGLLHDPEGGMEALYSIGVDRYLFVLLK
                     DLTLFPNHGQEWACIRRVPRWKMGFPERAPVAGLRTFFSDAFRMSP"
     gene            30192..31238
                     /locus_tag="PKN92_02150"
     CDS             30192..31238
                     /locus_tag="PKN92_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNZ47626.1"
                     /translation="MDINELFDLVHRENASDLLISAGAPPILRIHGQLYRHPADALNA
                     TEAQELVYAFLTPGQRERFERDKELDFSFEAEGKRRFRVNVYYQKGAVTAALRPIPEI
                     IPALEGLGLPDSLRNLAQARQGLVLVTGPTGSGKTTTQAAFIDLINSTRACHIVTVED
                     PIEYVHRHRNSIVDQREVGGDTHSFANALKYVLRQNPDVILIGEMRDLETIQAALTAA
                     ETGHLVFATLHTNDAMQTVDRIVDVFPAIQQQQIRYQLSLTLLAIISQHLLARKNGEG
                     RVLAYEILRANQAIAHLIRENKTPQLYTILETGMKAGMITMDRSVKDLYEAGKISYEE
                     AVTIMRNPRELQER"
     gene            31303..33564
                     /locus_tag="PKN92_02155"
     CDS             31303..33564
                     /locus_tag="PKN92_02155"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HNZ47627.1"
                     /translation="MGLLFALLTVAVSAADPSVCNGHALLYDSPAETWDEAFPLGNGI
                     LGALVWGDGNPLNISLDRTDLWDLRPVPEYYESDYSYTTMRQWEKEKRYKELIAKYDE
                     PYHRPAPTKIPAGRITLHFPGGTSFVEGRLPLDRACAEVRLKNGAIVRTWIHAREAVG
                     LIEVEHGAQAEFVLNAPPFAGVEDQRPRVDVVSGELSQLGYPAPVETKGENFTAFQQT
                     GWGGFTFAVYLCHKEHADGRLAAWSIASSRESDDPFALAKSRAEAALNQLDALRSSHE
                     AWWRTYWQKGQVSLPNIRLERQWYLEMYKFGAAARPDTPPITLQGPWTADDGKLPPWK
                     GDYHHDLNTQLSYWPCYSGNRLEEGYGFLEWLWQNKPQAEDWTRRFFDMPGLNVAMTT
                     DLELRQMGGWRQYTHSSTTAAWLAHHFYLHWCYSQDRKFLEERAWPWLSECAVFLEAV
                     TDERDSEGKRTLPLSSSPEVNDNKPNAWFPSITNYDNALIRWTFEKTAELADALGKAE
                     EAAHWRNCLGEMPPLAMNSRGALLVAQDYPLRESHRHFSHAMAIHPLGIIDRSQGPDA
                     ERIIRATLRDLDKLGTKLWTGYSFSWLGSLQARAGDGAAAEKALEIFSEAFVLRNSFH
                     CNGDQTDKGYSNLRYRPFTLEGNFAFAASVNEMLLQSQSGVIELFPAVPESWDNVSFD
                     TLRAEGAFLVSAVREGGQVIRAEIISETGAAPVIRNPWTGAVGTPDETSQDGRTKVER
                     DGDRWVLTPADRS"
     gene            complement(33584..34435)
                     /locus_tag="PKN92_02160"
     CDS             complement(33584..34435)
                     /locus_tag="PKN92_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ47628.1"
                     /translation="MARYIHLTDLHYWSLVCNPLRMLNKRALGTLNLLIRRRHQIKTE
                     RAAGLITLFQEMDIDAFLIGGDLTTTATEEEFRQAADFLGRLTMLAPVYLVAGNHDVY
                     TFESKRKERLEKYCQYWLPDEDTAAIDRLPGDVPLLRIPTVRPNLLSSRGSFQQDAAT
                     SLQTCLSEQQSQPMIVLAHYPFLPQTAAYHLGLNRGLGNGTTLRRILGGSEQPLLYLA
                     GHVHVFSHTRDPEHPNLEQVTTRALFYAKKDRPGGFTEIQAAGAQFSVYPWVYRDGWQ
                     REALSSP"
     gene            34757..37159
                     /locus_tag="PKN92_02165"
     CDS             34757..37159
                     /locus_tag="PKN92_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNZ47629.1"
                     /translation="MRDIKRVAVLGSGVMGAAIAAHLANCGIPSLMLDIVPPNLSDED
                     RKSKKNRDAIAAGAKGALLKAKPSPLYVKSYMDMIEIGNFEDDMAKISDCDMIIEVVI
                     ERLDIKKKIFAQVAEHRKPGSIVSSNTSGILISAMAEDMPDEMARHFLGTHFFNPPRY
                     LKLLELIPTDKTDPEVVKSVAEFGENVLGKGIVYAKDTPNFVANRIITFAMQYLMHEM
                     VNAGLSVEEVDALTGPAIGHASSATFRTADLVGLDTLQKVVGNVYHGCPNDERHDMMT
                     GPAWFDAMIEKGYFGNKSGSGFYKSTKERDEKGKRIILGYDPATGEYRAPIKPRFACT
                     GAVRSAGSMAEKLKIMHFSEDPGSAFLFKFFANMVQYAGNRIPEITDDIVNLDNACKW
                     GFAWEVGLFETWDLLGFDEVCSRMEAEGIELPPIAKALKEAGGNSFYKTENGTDLFFD
                     LASRSYKPVPSNPNELKLVNVRARATAVVEALDEASLIDLGDGILCAEFHCKMNAIGP
                     DIVAVLNKGVDLLEAGQFEGMVIGNQGPHFCAGANLMLVLGDAMQENWEGLENMVRGL
                     QGIGMRMKYCKAPVVAAPHHYTFGGGVEICQHADRVVLAGETYAGLVEVGVGVIPAGG
                     GTTEMVVRAFEYMPENIQPNDVFPFIRRAFETIGTAKVGTSGAEVVEFGYFRSSDIVL
                     PNYDHQIQKAKNVCRGLVISGYTPPRKPRLYALGASGKAAFEAAVWGMQEAGWASEHD
                     RLISTHLANILCGGDRLAGTPMTEQDFLDLECEAFVSLCGTEKTQARIQNMLATGKPL
                     RN"
     gene            37182..>38273
                     /locus_tag="PKN92_02170"
     CDS             37182..>38273
                     /locus_tag="PKN92_02170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNZ47630.1"
                     /translation="MENVYVVSAVRTAVGRSKAGSAIAHVRPDDLAAAAIGEAVKRSG
                     IPAERIQDCVIGCAFPEAESGMNVGRMALLTAGLPDTISGETVNRFCSSGLETMAIAG
                     AKIEAGMLDVAIAGGTESMSLIPMGGNKVMPNPTLVSINPRAYTSMGQCADNVGKDFN
                     ITREECDQWALESNLRAAAAIAEGRFKDEIVPLEVMGPGGKTYVFDTDEGPRPDTTIE
                     GLAKLKPAFAGPAGKGIATAGNSSQTSCGAAATVLASKAVVDELGLKPLARLRGYAVA
                     AGDPGYLGPAQVPAIKEAARQAGIDISDIGLVECNEAFASQTIYVVKELGLDHAIVNV
                     NGGAIALGHPLGATGAKLATQLIYEMKRRG"
BASE COUNT         8660 a         9918 c        10704 g         8991 t
ORIGIN      
        1 aagtggtgga aggaaatcac catgtgttcc atctgtatcc ggttatgctt tcaccggagc
       61 agtccggcat cagcaaagaa gattttgtct atcgtatgct gtacgaaaaa ggcgtgaagg
      121 tgggcacgca ctatattccg ctgcattgga caacggcgtt tcagaaccgt ggcttccggc
      181 gcggtcagtt cccggtggcg gaccgtgtag gcgaaaatct ggtgacgctg ccgatcaatc
      241 cgcggcagac ccgggaagca ctggattatc tgattgaaag catccgatca ctcatcggct
      301 aagtgttgcg gggaggaaac ttgcattcaa ctgctttgtc aaagtattgt ctttttgcct
      361 ggttcactgc ggatcagcaa aggaataacc gtttagaaca gggagattaa tcatgaaagt
      421 atattttccg gaagttgcga agcctattgc ttacgaaggg cccgactcca agaatccgct
      481 gagcttcaaa cactacaatc ccaaggaaaa agtgcttggc aaaaccatgg aagaccatct
      541 tcgttttgcc gtctgctact ggcatacttt ccgcagtggc ggtgctgaca tgttcggatc
      601 accggtgatg gatcgtgagt ggctgaaggg cgacagcgtc attgaagttg ccgagaatac
      661 gcttcaggcc gcttttgaat ttttcaccaa gctgggtgtg aaattctggg catttcacga
      721 tgatgatctt attccggaaa cgggatctct ggcagaatcg aacaagcaac tggatcatat
      781 ggtgaagctt gcgaagaaga tgcaaaaaga cacaggcatc aaactgatct ggggtacctg
      841 caatctcttt tcgcatcccc gcttcatgtc gggtgctgca acgaacccga atccggatgt
      901 gttcgcctat gcggcggcga aagtgaagaa agccattgag aacacgaaat atctgggcgg
      961 tttggggtat gtgttctggg gcggtcgtga aggctacgaa acgctgctca atacggatat
     1021 gggccgtgag atggatcatc tgggcatctt cctgaatatg gcggtggact atgccaaaaa
     1081 gatagggttc accgggcagt tttatattga gccgaaaccg aaagagccca ccaaacatca
     1141 gtacgatttt gatgcggcaa gctgctacgc tttcctgcag cgttacggcc tggacaaaac
     1201 cttcaaactg aatctggaag cgaatcatgc gacattggcg gggcatacgc tcattcacga
     1261 actcgaatac gccggagcca acggcattct gggttcggtt gatgcgaacc gaggcgatat
     1321 gctcctgggc tgggatacgg atcagttccc caccgatctt tatgacacga cacagatcat
     1381 gtacaccctg ctgaaattcg gcggattcaa aacgggcggc ttcaattttg atgcccatgt
     1441 acgccgtcag tccattgatg cggtggatct gttccatgcg catgtgggtt ccatggactg
     1501 ctttgccaga gggctgaaga tcgccgcaca gatgcacaaa gacaaaggct ttgccaattt
     1561 tgtgaaaaac cgttacagcg gatgggataa aggaatcggc aaggacatcg aaaacgggaa
     1621 ggtaggcttt gaagaactgg aagcttatgc tctgaagaaa ggcaacccga aaattgtgag
     1681 cggtcgtcag gagctgctgg aaaatatgct caacgactac atcaaataat ttctgttctg
     1741 atcgcgccgc ggcggcagtc tcgatgatgg aggctgtcgc cgcttttttt atgcttcccc
     1801 ggatcgccgc cagcggatca ggcaccattg aagcagaatg atggcaatca tcaccagggg
     1861 catcaggcgg aaggggagat tcaatccggc ataaatgccc accgcagcaa agcctaggct
     1921 gcctgtcata ccgcccagac cgactacgcc ttcgttgatg gcggcgcgtc catgttttgt
     1981 agtcggattt gccatgccgt aaaagacgct ggagaagaat gcgactccaa gattcatgcc
     2041 tatggcagaa aaagcgatga gcatgacgat tatactgtgc gtatatccca gcgaccacat
     2101 ggcggcacct gtaaaaagct ggatcacgac aagcatggaa aaacgatggt gccaccaggc
     2161 ggagcgcccc atgataaaaa aggtcagtcc ggtggcgagc cccatgatca cggcaagcca
     2221 ggaaaagcgt gaagccgatg cactgttcag ccaggcggag gcgtggcttt cccaaaggta
     2281 gcgcagctga cccgtactga tcagctcatc cagctgtttg ggaaaaaccg ctcttgtgga
     2341 gccgacggtg atatgggcgg caaaagtggc gcaccaggca ctatacagaa aagattcact
     2401 ggcacggtcg tgggctctcc gtgattgagt gctgtcactg ctgaggcaga cgaaataact
     2461 tttttccggt ggaacagatg caatcagtac gagcgataga agacaaagcc ccgcgataaa
     2521 aaggtaggga aaccggaaat caatctcata gaacggccct gccagcagcg gtcctgcagc
     2581 accacctgtg ctccaagcga cattgaccag actcatatgc cgtgcccgtg cttcggggaa
     2641 gggttccgcg ccgacccagg aatggtaagc cggccaggca aaagcagaaa aggtaaaagc
     2701 ggcaacaaaa aagaaaccgc agagaaaggg tgttcggaaa gcgggcatgg cgcccagaaa
     2761 aatcagaaat ccagtaacac cgaatagaga ccagttcaat ccatgccgcg cccgtgcgac
     2821 aaaccgtgag gaaaacaggg atgccagtgt atagccgagc gacgccaggc cgatgaaaat
     2881 gccagagagt gtagcaccct ggttcatctg attgaacaga aaaaaaggca taaccagtaa
     2941 ggcggaattg actgcaccgc tgaaaagaaa ggcgaccagc gccacccgct gcggcggtgt
     3001 cataccccag cggttccagt cggaagggga ggcgggatta cggtctgcac agtgagacat
     3061 ggttttactc ctgaaaaaat aaggcacaat agttccgaac gagaggcggt gagaccttta
     3121 ggagggaagc cgatcagaga agaaaggctg catcttttgc atgcagcccg gcgtattata
     3181 gcagggttcc ctgccctcct gcaggcggcg gacttttctt cagatgcctg aaggcgtgcg
     3241 gcgttgccat gcgcccttgc ggggttcgtt tcagaaaacc tatctgtatc aggtagggtt
     3301 cgtgaacttc ttccagggtc tgccgttctt cgccgacagc caccgccaga gaagccaggc
     3361 cgacagggcc gccgccgaat ttatcaatca gcacttccag tatctgcctg tccatatcat
     3421 ccagcccgag cgcatcaata cgcagcagtt ccagcgcccc ttccgccagc tctctggtga
     3481 tgattccatc gcctttcact tgcgcaaaat cacggacacg ccgcagcagc cggttggcga
     3541 tgcgggcagt gcccctggaa cgtgcggcga tttcaagcgc tccttcgggc tgtacgggga
     3601 catcgaggat tctggcggat cgggtgataa tacgctcaag ttcttcagga gtgtacattt
     3661 cgaaacggca ggtgtcgccg aagcgggcgc gcagcggcgg tgtaagcagt ccggcacggg
     3721 tggtggcacc gatcagtgtg aaggggcgca agccgatttt catggatcgg gcagtgggtc
     3781 ccttcccgag catgatgtca acctcgaagt cctccatggc ggaatagagt gtttcttcca
     3841 cagcgtgatt taaccggtga atttcatcaa taaaaaggac atcgaaattt tccagactgg
     3901 tcagaatggc ggagagatca gcttgacgct ctatgacagg acctgagctt tgcttgatat
     3961 ccactcccat ttcattggcg atgatccggg caagggttgt tttgcccaga cccggagggc
     4021 cgctcagaag caggtgatcc aggggctcgc tccgttgctt tgccgcttca atcgcaatgc
     4081 gcagcttgtc tttgatcggc tcctgtcctg gaaagtcgtc aagccgtttc gggcggatct
     4141 gatcatcata agaggcatcg tcttcaacgc gccccggtat aattacctca tctctggaca
     4201 tggcctgcct ttgctaggga acgtaacgct tcgcgtacga gttcttcatc gggtgcctca
     4261 ctgcccagca tctttcgggc gtgggcgctg gctttttttg cctcctgggc agtgcagccg
     4321 aggctgatca gcgcctcata cacatcatca tcgccggttt cctctgccga ctgaggatct
     4381 cccagcaggg cattgagctc cggatcctgc cccatgcggg tcttcatttc caggataatg
     4441 cgctgtgccg ttttcttgcc gacgcccggg acgcgcgaca tggcagtgat gtcatgctcc
     4501 tgtacagccc gggcaaaggc actgacggac agggtggaca aaatggacag ggcaactttc
     4561 ggaccgattc cggtaatact catgcacatg atgaacaaag cacggtcttc ttcggtcaga
     4621 aatccgtaga tctgaaaaat atcttcccgg atgtgacaat aggtgagcag tgttgcatgc
     4681 tgaccgacag gcagcttccg ctgagcagaa tcgggaacaa gcacgtcgaa gcctactccg
     4741 ttcacatcca gagcaatgcg gttaacggat gaggcgacta cctctccacg aagaaaagcg
     4801 aacatgcaaa gaatcctttt ccatccggag cgtcagttgg agtgctttgc caggtattgc
     4861 cccggcgccg gcacagacag gatctcacca gataaaatca ttaaaaaacc attttatggg
     4921 tgtcagaaaa gagtttttgg tctgtcgacc gtttttagcg gaagcagtgc ccgtcccgcc
     4981 ccggtgtgca aaagcataat tcagtatacc gatggtcggc gcccattcag gagtgttgat
     5041 ggcgtcgggc agttcattga atccgagagg cagtccgatg cgtgtggaaa ttccgattgt
     5101 tttttccaga atcttatcct gattactgac ggctgcaccg ccgcctgtaa taatgagccc
     5161 tctgcttagt ttcatgaggt agttgtttgt tttcagatag tcctcaatca gtgcggcaaa
     5221 ctgcttgctt ctttcaaaaa taacggcatc aagtttgctt ttggctacag taccgggtgc
     5281 gcctgtaacc gtcttatgca gtttgatggt gacgtcgctg tccggcgggg cgatgctggt
     5341 ggaggacaat ttgaatccgc cttttgtcgg tgtctcgaac agatcgatgt taatgccgta
     5401 atcgagcagc agttccacgg cttcctcgaa atagacattg agtacggcgg caatatcacc
     5461 cacgtaatgc ccgctgcccc atttgaagac ttcggtggca atcagccggt tatcgttgta
     5521 gacggtgata ctggtgctgg ttgagccgaa attaatgacc gctgcaccca ggcggatatc
     5581 ttccagcgtg aggcagccat gggcggctgc caaaggcatg taaacaaaat cggcaatctt
     5641 ctttttctga ttttccagac aggttctcag attgttttcc agaaatatgg acaaacgggt
     5701 aaagtggatc cgccctttca gaacatgccc ctgcattccg atggggtcgg cgacacgtat
     5761 atcgtctaca gtccattgct ccgatttgat gcaggaaacg ggacgggatt caggagtaac
     5821 gcattccaaa gaagcattgt gtcttgcggc ttccagatgt tccggtttga tgataccgtt
     5881 ttctatttcc actttaccgt catgaatgcc cgacatctcc gtgctgccct gaatgccgca
     5941 gtaaagataa tacgtttttt ctttcgccat tgcctgggct tgtgtcagtg cctgattggc
     6001 gagttttcct gccaggttga tatcctgaat ggcaccgaag tgcacggcat cctgagtcgg
     6061 tacggagctg tagcccagaa tgtgcgtttg tccgttcctg aggcggctgc cgataattac
     6121 acggatagca cggcttccaa aatcaatgac acctacgata tctttgttct tcgacacgac
     6181 acacaatctc ctttttctat ctgaacacct ctggaaaatg ccgccgcccg atggttcagc
     6241 gagcatcctc atccgaacgt atgtaattca aagcgtttgc cagcatggct tccatctgca
     6301 gcagagaagc gtggtgccgc tccattacag ttctggcatc ggttatttca ttttcaatta
     6361 atttccgttc cgtttcatat cgttttttgt cgtcatgcag atcatccacg tgggtgagtg
     6421 cagcagccac tttttgtgcc agagcctccg catcggtgct gatccggctt tcctcagcaa
     6481 gggcaccttc tttcctttca tatatttttg ccagttcctc attttttagc gcgcgcttct
     6541 tttcaagctc ggaaatctgt ctggacaatt cttcaatatc cgtcagtgtg tcgccgactt
     6601 cgatatcaat ttgcgcgatg acttcccggg cgtgatttcg ctcttcttca cggcattcca
     6661 gttcgtcctg catccgcgca agcgcttcac ctgccattat tgttttttct tcgacttcct
     6721 ggatggcgcg ggcattttct tccagatgct gctcagcctg ctgcacggca ctttttgcgg
     6781 tttcataggc ttttttatcc agagacagcg caagctgcag cgcaccggcg cgggcatgat
     6841 caatgtcctc gcgggtgatg gatgcctgaa tgtgctcaag aatctggtgg ctgtcctctt
     6901 ctggctgaag gggatgcgaa ctccgggatt ctggaggcgt atctttttct gaaggagagg
     6961 cggattccag cgcttcttct tctgttgcag ataaagactc ctgcgcttga gcggaaccct
     7021 cgtcgtcttc cgcaaaaaag tcttcctgac cttcgtcttc ttctattact agcgaaaaga
     7081 gatcctccgt cacttcctcc tgaatctcat cctccagtaa ctcctcattg ttctcgggat
     7141 cgggatattc ctcttcctca tgtgtatttt cctcttcata gatttcatct gtttgggaaa
     7201 aagaaatgtc ttcgggagat ttctgctgtt cggggaaaag aataagcgtg tcttccggcg
     7261 ccgatgccgc agacgggggt gtatcaaaga aatcgtccat taagggcggc agattgatgg
     7321 acagtacctc cggttgcgtt tggtgtttta ctgccggggc gttatctgcc ttcccgtcca
     7381 tttccgctgc atttatttct ggaaaacatg tctggtccgg tgcatcagaa gtttgtgaat
     7441 cactgacctt ctcttcgggc tcttcgcctg aggtttcttc cgaaggctcc agatctccgt
     7501 cttcggtctc ttcacagggc acctcttctt ccgcctccgt ttcttcgaaa gactcctgtt
     7561 cagggtcctc ttcttccggg gcatcatgag cagtgacgat ttctttttct tcggtgtccc
     7621 gtgtcagcgg aatgcctgct gctgtcattg acgacacggt cttttcataa agggctgccc
     7681 attttcggat gacaccgtca cgcacttttc cgacaatgat atccccgacc attaccgtca
     7741 gcgcctgata actttcccgg agaaaagaaa ggcattcaaa agtttctcct gctgcatgat
     7801 agctgtgcag gatccttccc atttcctgaa tggtattgcg aacagggacc cactgattct
     7861 gcttgcagat taaataggca ttcagaagtg cttcccggca attatcatac cagagctccg
     7921 catcattttg atgataggca tcttcaaggc atcgttgaaa gggtgccagt gcggcgacga
     7981 gctggctggg gtctgtcttt gcagtgtttt ctgaatcgga cgggggtact gtgctgtccg
     8041 gataaacgtt catggattct tcctcctcct cctgcgcttg agaagaaacg gggggctcat
     8101 tttctgtctg tgtggcggca tcgttgtcat gattgcgggc gcgattgaac atcgtccaca
     8161 aactcttttt tgcaaaccgc acgcctgaaa tctccgaaaa tgaagggttg tcgggaataa
     8221 aaacaatata ttgtgtgtgt gctaaatctt atccactata tgatgacaga ttccccgaaa
     8281 aattgcaata ttttacgggg ctttttttcg cccggcaggg ggtgattttt ctcataaaaa
     8341 ggcaaaatct ttgtccaatg gtggatcagc ggcgtgtttc cataaaacga tcccacagat
     8401 cccgctgaat ccatacatga aaaagaacgc ccggacgcaa cgcctgaaac tcaacgaaat
     8461 agttaccttt caggcgggct tccaccgcat catgcagcgc ctgaggtgca agaatcatgg
     8521 cagcatccag atcttcaggc aatgctgtcc accagccgac ccgggtaaaa gacttcaggt
     8581 accagggcag cggccagtag tcgccgatag gctgcaccag attgatacgc atattttttc
     8641 cctcagaaga gacagcggcg atttgttcaa cacgatcggc aagcttgagt attgcccggg
     8701 acggatgggc gtagacatag ggatttcggg tgtcggcgtc atacacaaaa tttccctgat
     8761 agctctgcca gcccaggtgt gctgcaccgg cgaggagcag cgctgtgacc ggaatccgca
     8821 cgagcagagg gaaacgtgcc gcagtgagcc gggccgctcc aattccggca agcagcaata
     8881 gcccgtgaaa tgccggaagg atgttccagg gtgttttata gggaatggca ctgaacagcg
     8941 ccagaagagt cagggcatag acaagcagaa aaggctgaaa ccgggtttgc gctgtttttt
     9001 ctttgttgtc tttgcccgga aagacggtgc ggtaaagaat catcagtgcg gcgataaagg
     9061 caagaaagag caccaatccc tccgtccagt acgggcctgt ctgccggtgg gtataactca
     9121 ggagctgcag gtagtagtac cagggtttgt cgtggatacc ggaacttccg gctccttcag
     9181 cccggtgcag gtagtgtgta taggtgagga tggaatcaag gggaccgcgg gcatgcgtca
     9241 aaaaagacgt aaaaaatacg acagatacgg tcactgccgc agcgcttgcc agaacgatat
     9301 gaagtttccg ctgccgggta aaaaaggacg ccgccatttc cttgaaagac cgggacgggg
     9361 aacttttgta cagccatagc gtgactgcca gccctgcgag agcactgaag accataagaa
     9421 tgcaggtttc cttagtggcg tgaatcagtc ccaatgcgac gccgaaaagg agtgcccgaa
     9481 aagcagaagg ctttttaaaa tagagccatc cgaaactgaa ggcggcctga atgaaacaga
     9541 ccagcagtat ttcctgcaca taataccggc tgtaataaac catcacaggg gagagcgcca
     9601 taagcagggc agcccagaga acggcagccg tgcccatcag atcccgaaga taccagagca
     9661 gtgcgatgag cgtgagtcca cccagcgctg tcagcagccg gtaggggacg atggttgaat
     9721 cggtgaagtc ttttgcgccg ctcagccaca ggaaaggcag ttcaaggtaa tagagcgtcg
     9781 gtccgtgatg ttcaaaagga tcatattcat aaaaaccacg gtcataaaga gcgccggtct
     9841 tcacggcctg attggcttcg tcgccgtgaa gaggccgtct gtccagctgg ggcagccgaa
     9901 aagcagcgcc cagagcaaga accagaataa acagggcgat gccccaaagg agtgaacgct
     9961 gatacacgct gcttctcttt ctgtgatctg agttaaatgt ccggaaacag tgacccacgc
    10021 ccggatcaat cggagtcttt tctgtgttat atcataatgt gctccgccct tatcaggcaa
    10081 ccggctttat ttctccggag aagagcaggt aaaatggtca gcatatgttt tatccggcag
    10141 tccggtccgg gcatagagaa cggatagggt tgtgcggaaa aaaaaggaat attatgaata
    10201 tactcggcat ctcggctttt taccacgaca gcgccgccgc actgctctgc gacggcgaga
    10261 ttgttgccgc cgcccaggaa gagcgttttt cccggaaaaa acatgatccc tgttttccga
    10321 agcacgccgc tgcctattgt ctggaagagg gcggactcga tatcagtcag atcgattatg
    10381 tcgcttttta tgacaagccg ctcgtgaaat ttgaacggct gctcatgagt tatcttgctc
    10441 tggcgcctaa agggtatctc tcgtttctga gccagatgcc gtcgtggctg aaagaaaaac
    10501 tttttgtacc gtccattatc cgcaaggagc tgggctatga cgggaaaatt ctgtattcca
    10561 cccatcacct gtcccatgcc gccgcggctt tttatccgag tccctttgaa gacagtgcta
    10621 ttatcacggt tgacggcgtg ggtgaatggg caaccaccac ttacggtatt ggcgagggcg
    10681 cggatatccg tattttaaag cagattaatt tcccccattc cctcgggctg ctttattctg
    10741 cttttaccta tttctgcggg tttaaagtca acagtggcga atacaaattg atggggcttg
    10801 cgccttacgg cgagccgcgt tatgtggata tcatcaagga aaaactgatt gaaatccggg
    10861 acgacggttc gttcagaatg gacatgcgct attttgatta ctgcgccggg ctgcgtatga
    10921 ccaaccggga atttgcggaa cttttcggcg gaccggagcg cacaccggaa agcaggatct
    10981 cgcagcgaga aatggatctt gcacgcagtg tgcaggcggt aacggaagaa atccttttaa
    11041 agatggcagc gcatgtccat caggagacag gcaagaaaaa tctggtgctt gccggcgggg
    11101 ttgccctgaa ttgtgttgcc aacgggcgga tcctcaggga gagtcctttt gcgtcgctct
    11161 ggattcaacc ctgtgccgga gacggcggca atgccctggg tgccgctctt tttgcctggc
    11221 atcaactgct cagaaaagag cgtgttcccg atgcacgcag acaaaaagca acctatctgg
    11281 ggccttcctt ttccgatgat gcaatccagc aattcctcga cgcgcgtaaa gtgccttata
    11341 cccggctcgc acccgaagaa gttccggcaa aagtcgcgga gcttctggcc tcggaaaaag
    11401 tggtgggctg gcatcagggc cgcatggaat tcggcccccg ggcgctgggc gggcgcagca
    11461 ttctgggcga tcccagatcc cgggaaatgc agtccgtatt gaacctcaaa attaaattcc
    11521 gcgagtcctt ccgtcctttt gcaccgacgg cccttgccga tcatgccgat gactattttg
    11581 atctgcgcgg gcatgaaagt ccctacatgc tgctggtggc ggatgtactg gaaagccggc
    11641 tccgccatct gagcgaggaa gaacagcagg tcgccggttt cgataagctc cgtgtcatcc
    11701 gctcagagct gcccgccatt acccatgtgg acaactctgc acgggtacag acagtccgtc
    11761 gggaggatca tccgctgtat tacgacatga ttcgggcttt ctatgagaga accggctgcc
    11821 cggtggtgat caacacttcc tttaatgtcc ggggcgaacc catcgtctgc acgcccgaag
    11881 aatcgtatac ctgttttatg cgcacccgta tggattatct ctgcctgggt tcttttctgc
    11941 tgaacaaaga ggaacaggag gcgtgggagg aagaaggtga ctggcgcgaa gagtttgaac
    12001 tggactgatc agcaggagat gtaccatgct tgatatagat acttccgacc gcaaagagca
    12061 gcgaaaattc ggttttgtta tggcggcggc gattgtgatt ctgggcggaa tacgttttgc
    12121 cctgcacgga tttacagcca taccctgggc ttttcttgct gtagcggtgc tgtttgctgt
    12181 ccccgctgcg ctgtttccgg gggcgctccg ccctgttttt gtggtctgga tcaaattcgc
    12241 actcgtgctg aactggatcg tgacccatgt catgctgacg ctggtctatt acctgatcat
    12301 tgtgcccatg ggcctgatca tgcgtgttgc gggcaaggat cctcttaacc ggaaatggct
    12361 tcccgcaaca gagagttact gggaagagcc ggaagagcag cccgaagaat ttgagcggta
    12421 ccaccatcag ttctgagtgc cgtgccggac cggcgggctc ttctgccgca gcagtcagac
    12481 aagatcgggc acgtccaacc aggtgcgccg ctgccagctc tccatgccaa gttcggcaag
    12541 ctgaacaccc tttgcccctt ccagcagatt ccagcggaag ggtgtgtccg ataccacatg
    12601 tttcaggaaa agttcccact gcgcacggaa agcatttttg taattttccg tgtcgggcat
    12661 gcatgtccaa tggctggcgt attccagagt cctgcccaga tccgggttcc atacatagcg
    12721 gggcgttgcg gcggcaggct ggatatgaca ctgggtcagc ccggcaacgg cagagccttt
    12781 ttctccgtcc acatggagcg tcagcagatc atcacgtcgg acacgggtcg cccaggagga
    12841 attgaactga cagacaatgc ccggttctgt cacaaaagtg gcataggcag cgtcgtctgt
    12901 gtccacctcg aaaatctgtc cggactcgtc ccagcggcga cgcacatgga tctcgcccag
    12961 acagctgaca gagttgatgt tgccgaagag attatcgatg acgtagcgcc aatggcagag
    13021 catatccaga ataatgccgc ctccttccgc ttttctgtag ttccacgaag gccgctgcat
    13081 gggtacggcg tcgccctcaa aaacccagta gccgaattcg ccccggaccg acaggatccg
    13141 cccgaagaag ccggtatcaa taagccgctt cagtttcagg agaccgggca gccataactt
    13201 atcctgcacc acaccatgct ttacgcctgc ttcttccgct gcatgataca gcgccaacgc
    13261 ctcatcgctg tttgtggcaa tgggcttttc acaatagaca tgctttccgg cacggatggc
    13321 tttgcgggtt gcctccacac gcagcacagt gctgagcgca tcaaaataaa cactgtactt
    13381 gttgtcggag agggcggcat cgagatcggt cgaccagggg agcccgccgc aggcttccga
    13441 caatgctttc agtttgtcct cattgcgccc gacaagaaaa ggttcgggca tgatgcagta
    13501 ctctcctgcc cgcagtccgc cttcttttct gatttccata agagaacgta tcagatgctg
    13561 gttggtgccc attcttcctg tgacaccgtt catgataatt cccagtggtt tgtgcagcat
    13621 ggcttttatc tccttgtctg tccgggcggg acgtatgtgc tatttttgct tcaagctgat
    13681 tctaccagcc ctgcaccagg aaggcaagcc ctgtcgggaa aaggcgaaag gaatcagata
    13741 ttccattccg gtctgcgtgc atacgccaga aaacggttgg catcttcatg ctccgggaaa
    13801 cattcctgac gggcatccca gcgggcatat ccttttatct gtgtggcaat gcccgccagc
    13861 agcaccaggc tcatggagcg gtgcgctgtt tccgcatcac atatggtgcg tttccgcgtc
    13921 cggatgcagt caagaaaatt gccggcatgg atcgggcttt tgtaggggtg tatttcctga
    13981 gggcgaatgg agatatccat aatatttcgg ggtgatgcca tgatcccttc ccggtcgacg
    14041 gcgagtgtgc cttcggtgcc gataaaacag gcgccccctg ttgcgccgac ctcaatttca
    14101 gggtgggcac agcaatgcac tttgagtccg ccgggataca gcagaacagc atggtcttct
    14161 tcataatcga tcgcttccgg tccgttgaat tgatgttcaa gaacccactg caccacatcg
    14221 aagtggtggg gcccccagtt gggatcaccg taggcgaaga tgccgcaatg gacattccct
    14281 ccggtaaaag gctgccaggg tgcagtgccg agatagaggt tccagtcaaa accttccgga
    14341 aggggctgct ctttcaggcg tgtgttctgc atgttggcgc tgaaaccgcc gcccgggctg
    14401 aagctgtaga tttcacggag ctcaccgata tagccgttcc ggatgagctc ccaggcacgc
    14461 tgaaacttgc cgccgtattc cgcttcggag cgctgctgag tgcctgcctg atagacacgg
    14521 gcgtaacgcc gcatcgtatt ggagagaagt cgcccctctt caatggtaag agcaaccggc
    14581 ttctcactgt acacgtcttt gcctgcccgg gcggcaagaa ttgccatggg ggcatgccaa
    14641 tgaaagggga gtgccatcag cacagcgtca atatcttccc gtgcaagcag ttcccgaaaa
    14701 tccagatagg cacggcaccc atcgtaagag gtctgtcctg tagtgtgtgc ataatggcgg
    14761 ttgacgcggt cagcagcatc ttcgcggcgc tgcttccaca catcgcagac agcctgcacc
    14821 tgtacttcgc gcctgcccag ataaccgccc gccacatagg tccaggatcc gccgaagaga
    14881 tggccggatc cctgaccgcc cagtccgatg aatcccatgg cgatgcgttc tgacggtgcc
    14941 acagcgcctt ctctgcccag tgcccgggcg gggacgatga gcggtgcgga aagggcggca
    15001 caggcggtgc ccagaaaatt acgtcgtgat aagtgttgtt ttttcatgag gatgagtccc
    15061 ttcgctgcag aaagatccgc acgccttttt tgcttgatac ggcaatatcg tccaatccgt
    15121 ctccgtccac atcacagact tcaaactggg tgcctacgcc tgaatcttcg tggatcacgt
    15181 ggcgtgtcca ggaaggattg ccctgcatat cccgctgaag ctcataccag cagagcaaag
    15241 cgggctccag agcaccggag tcttctttcc cgtgcgcata aaaacgcttg cctgtgatca
    15301 gatccgccag accgtcatta ttgaggtcag cctgtatcag tgcatgggtt gccgatatag
    15361 aacggtcgat ctcgtgtctt atgaaagagg gaagtgcctg cccggacggc tgctgttcaa
    15421 accaccagat gccgtagtcg tgggcggaac ttgtgatgat atccataagc ccgtcgttgt
    15481 tggcatcata gacgagcatg tgcgcgcaat caggtcccag atcggcaggg tagggcgtcc
    15541 agtccggtcg ggttgtctca gttggtcctt cccaccagca cacggtgctg agcacatcag
    15601 tgcgtccgtc tccgttaaca tcgcctatac cgagtccgtg tccgaaaaga gcgaactgtt
    15661 ccagctgatg tgtgaagggg taggcgatcc agggagcagt cacatcctgt ccgggagtga
    15721 gccaggtaat gcgcttgttc atggcaaaga tgagctcgcg ctttccgtct ccggtaatgt
    15781 cttcataaag caccgtttca ccgcaggctt caacagctat cagatattcc tgccagtgtc
    15841 cctctgtttt tccggggttg cggtaccagt agaccggtgc gccgggaaag cccaccacaa
    15901 tcacatctgc ccaaccgtct ctgttcacat cttcggcaaa gacggcaaag gcacggctgt
    15961 attcagtcag ggcgtcatag gttccgggcg ggcggatttc atgggctgtc cagccgggtg
    16021 ccgcatacca gtaatcgccg acgacaacat ccggcagacc gtcccggttg atatcggcaa
    16081 ttgcaatgcc ttccgagcgg aatgcagtat caatgcatat ttctttaaaa cggaggctct
    16141 tgcccgggac tgcttcggca ttcagtattt tgcggacacg ataaagcggc tccagcgcat
    16201 tggctgacag cccggctgcc tgtgcctctg caaaaagggc agggaaagcc tgtgaaatct
    16261 ctgtccgatt ggtgtccaga agcgccaggg caatggtgcc ccaggcaacg gcaccttccg
    16321 cttcggtgcc ttctctgtga aaccacgctt cagcaagcac gagcgcatcc ggagaactgc
    16381 accggttgag tgcggaaaga agtatccgtt tttcctgcag attttttgcc ccgtccaggc
    16441 attcccgaag caatgccgtg cgggctgcat cgttcatttc atccgcctgc gacagggcag
    16501 ccagatttct gatcagaggc agacgcatcg tatcggatat gcctgatttc agcaatgcct
    16561 gtaaaagcgg aagggcgtcc gggttccggg tacgggacaa cgctttcagc gccgcttctt
    16621 tttcttccgg agtgcctgtt gctgccgcat cgcccagaag agcgagcagc tgcggagcgc
    16681 ccactgtgcc gaaaagagag aacagggcac agcgctgcgg cgtattgctc cgcgcgtgac
    16741 atgctgccag agtctggata tatgtttcat cgctgcccag gcgggcgacg agcttctgca
    16801 aggtactgaa acaggcggca gcgtcttccg gagagtccga ctgatgccag acatcggcaa
    16861 ggagcgcagc gtctgaggca ttaccgactg ccgccagcgc acctgctgcg gcaatgcgca
    16921 ctttagcggt acgatgatgc accaaacgcc gaagttccgg tgcgacactg tcttcccggc
    16981 gcagttccag cacctttgcc actgtctccg ttactgccgg atcggcgtga tccagatagc
    17041 ggacgagtgc ggctgctgta ccggaagccc gtagggatgc aagcgcctga agggctgcct
    17101 cggcaatggc tccgtctttg cgcactgccc gttgaataag tgcggggaca gcatcgctgc
    17161 tgtccagttg tatcagtgca tccagtgcat ccagtgctgt ttgctgatcc tccgcttcta
    17221 tccagggaag gacgaagggc aaagcggaca tgttttttcc ctggcccagt accgcaataa
    17281 gcgcgcagcg cctgctgtcc tgcagctgat ccagcgcttt tccggcacgg cgaaccgctt
    17341 ttttgctgtt catgtgcggc agagcggaaa gcgccccctg ccaggaagcg tcgctgtccg
    17401 acatcaatgc ctgaaaaagc agatcggcac tgttgcgccc tcccgtcaga agcatgcctt
    17461 tccaggcggc ggctgcaccg ggactatcgc tccaacggga cagatactgt ctgtaagaac
    17521 ggaacgcctc ttttcttccg gatgcaggca gggtatccgt gcacgccagc agtgcttcct
    17581 gcacgagagg aagacactgt tccgatgctt tgtcagccgt atgccacagg ctgcgaagag
    17641 cgggcggccc gccgaatttt cccagtgcaa gcacagcggc acggacaaca gagggatctt
    17701 tgtcgtccag tgcggacagg agcaacggca gatccgtttt ttgtcctgat tttcccaggc
    17761 tttcgagtat accggctttt acagcaccct ttgattttgc cagccccgag cgcagcgcat
    17821 tccgggcttc ttcataaggg agtgcctcca gcgcaatgcg tgctccctgc gatgtttctt
    17881 catccagcag atacgaagcc agcagcggaa tgcatttgcg gcttcctgtc tgtgccagac
    17941 gggcgcagaa tttcgcttct tctccggtct tttccggctg ctccagaagt gcgaggatga
    18001 aagactcctc atccattccc ggtacccaga cgggtatgcc tgctattctc tcgcttctgt
    18061 catgatcaag tgtgacggcc aggatattga gtgttaccgt aggatcgatt acagtcgcgg
    18121 taatgccgca gagcggcaca tcagggcggg ggtttcgcac agaaaaacgc cagaaagaaa
    18181 ctgactttcc cgtatgtatt ccgccgaggg tatccgtgaa ggcttctgct gcaaaaggca
    18241 gcgggcccgt cagccaccag tcctgaatgg agatgccgtt ttgaaggggg atttcctcac
    18301 ggtttccgtc ttcataaagg agcgtgtaac tgccgacccg gtctccggca ttttctgtga
    18361 agtgaaggaa atgaatactt tctgctgtcg tggcggggaa gtgtagcgtt ttctctgagc
    18421 ctgccctgac acggagcatt tgctccgtca gctgaaagcc cacgcccccc agagtagctg
    18481 tgccgggcat cacttgctga aaaaaggccc ggggattacc ctcacccagt tcaccaaaag
    18541 aaagggtaca agcttcgctg tttccttcac agggaaagaa caccacgctg agagctagcg
    18601 ccaaaaaaca gtgcatgcgt aaataaaaga tcattttgaa actttccata taaaccgatt
    18661 gtgatgtggc aacacgatga tacaatgttt tattattgtt tcacagagca gcagacaaac
    18721 acgactttta ttttgtacac ttttcaggtt caaaaataag ggttccatta ttctttcctg
    18781 tctccttttt ccggcgcacc tttcatgccg ccttccgatg ctaatctgtt ttcttgcggt
    18841 cggcagtctg cttcaaggca cgtccattgc gtcagatatc gacttgcctc agatgctgta
    18901 cgtctggcag cgcctgtgga cagacgaagt gaaatcggca gtaagggaag cctctggcag
    18961 tgcagaggga ctctgcgttc tctgcgggga gatagccggg aggggggaag gctggcggtt
    19021 tcaccgggtg aacgtggatt gggcgtcact ggcaccggtg aaaggcacac tctggcttgt
    19081 tttccgtatc cataccggtg ctttggagag tgaacattgg gatgcttcca cttttgccgg
    19141 acacgtacag aaaactttga atgacggagc agacgcgggc tgtgtcattg cgggcattca
    19201 aattgattat gattgtccga cagaaaaatt gacgctttat cagaactggc tgcaggtgct
    19261 gcgggggaaa ctggcgggaa tacctgtttc cattacgggg ctgccggatt ggctgacatc
    19321 gagaaggctt cctgaactgc tcgccggtct cgatcatttt gtgctgcagg tgcattctct
    19381 ggaactggac agggaatcag ggcgccagcc ggtgctcttt gatgaaacta aaatgatgcg
    19441 ctgggtgggt cagatgaaca gcctcatgct gcctttccgg gttgcgctgc ccacctatgg
    19501 ctggcgtatt gcttatgacc ggtacggaca gccgcagcgg cttgaggcgg aaggtatcca
    19561 tgaagcggta gcgggatgga cgtaccagga cgtatctgct gatccggcag ttcttgcagg
    19621 agcagtccgc aaactggctg ctgtatccgg cggtaactta aaagggatta tctggtttcg
    19681 tatgtctctc ccctctgagc gcatgaactg gtctctgaaa accctgaggc aggtgatgaa
    19741 aggaggtgcg ccgcaggcgc attttgaagc ggaactgcgc ctgagcgata atcatcttgt
    19801 ggaattgtga ctcgcaacgc atgatgcagc ggggctcggc aggctttgcg tgcgcctgca
    19861 ccttcagtcg ggcagcattc ttgcctccga tttaatgcgt ggttttcgcc agctaaaaaa
    19921 tacggaagag cgttatatta cagtttcggg cccggtgtat tccgacgggg aaaaacatat
    19981 ggcaggctgg tggcttctga gtggcgacag cgattcggag gttccggcat tatcagtaga
    20041 ggagatcggc ttatgtcctt gaaaaagttc tgttttcttt cggtgcttat gaccgccagt
    20101 tttcccggta tggtctttcc ctgtgcctgg gagcctatgt cctcatattt tggtgaaggc
    20161 agcgataaag atctttatta cctgccggat acgcattttg attcggaata tctccgtata
    20221 accgatcagc ttctggattt aactgtggaa gaggcatatc gcggggcgca gctgtctttt
    20281 tatatggatc tgccgaacta tcttacccgg atggctgtgg cgcagcagcg tgctgaaacc
    20341 agtgaagcag aggcgcttgc cgatgctctg gctgagaatg aatccaaaga aagttttttg
    20401 gaggcgtatc gctttctccg ggatcttgtc aaccgttatg cacagcgtca attggatctt
    20461 cggattcatg gagagcaggg ctggacgttt caggaagagg agcatccgga aatgctggag
    20521 cagcggcagc tgcaggcact tctggatgtc tgcccggatg agattccgcc gcaattcaga
    20581 ctttatcttc agggagccgg ggcttttcat tcaggcgact acaggcgggc acagtcctct
    20641 ttcagccaga ttcttcagct gcccgaggca gagcgcaccc ataaaacggt ctgggcgctt
    20701 tttatgctcg ggcgctgtgc tatggaatta aaagcgctag atgaggcgga atcttttttc
    20761 catcaaacac agcacgccgt tgatgacgga tttgctgatc ctcttaatct gcacggtgaa
    20821 tgcttcgggt ggtttggaaa gatctatctg gagcggggcg cctttgtgga agcggtgcat
    20881 gcttatgttg aatacaggta tcagcctgaa aaatgggaga tgggcaagga gtcgctcttc
    20941 atcctttttc agcgtatctt cagtcaggaa accgatcttc gtgctctggc gcaggatgat
    21001 ctttcccggc agctcattac agcctggttt acgagccaga gcaggccgaa ccctgaccgg
    21061 gtaaaacggt ggctggaaag cgtcgcattg ctgccccggc aggaagaact cccgggcgca
    21121 ggacagcttg cctggctggc gtatcatcat ggcgacatgg aggcggcagc ccgatggcag
    21181 aaacaaagcg atgagcacag ccttgccggt cgtttcgtgt atgcgaaact gttactccgg
    21241 gaaggaaagc ttgaagaagc cgatgccctg ctgaaagcgt tggttgagga tacgaaccgg
    21301 gacaggctgc ttctttttga agggcattat ccccgggtga caggtgcttc tgtccgtgcc
    21361 gctgaagcgg cggtgcttct gaagcaggat gactatatcg gtgcattgaa aaactttgct
    21421 ccggttttcc tggaggacgc tcagtttctg gcaaggagga tcctgacgct ggatgagctg
    21481 gatcgtgcca tggcagactt gaagtcgatg gatccgcagg aagtcgcgga tactctggac
    21541 accggggatt attttaatga gaccgagtat caggaagcgc tgagcggatt cctgtgtgcg
    21601 ctggaaactg ttcatgcaca gcggcttgca cgcctgaatg actggtctga tgcggcgcaa
    21661 catttccagc aggcggaggc gctgttgaaa gaagaagggg atccggagga cgcggaagat
    21721 ctgtctgccg ctgcacagca gatagccgat ctgatgcgcc gaacaaagga aagtgcactc
    21781 agtgaccgtg aaaaagcgca gctgctgatg gatgcgggtg cactgatccg gcaatacgga
    21841 agtgatctgc ttggcgaatg gtggctgttt caggaacccg gtgcggagag tctgacggag
    21901 ggcgggcgag atctgctttc cgatgacgga cttaaacgga tggacgcatc ccggaatgta
    21961 tgcggaaggc agtacagttt ccgtttcatt gcctctgatc ttatgaaaga ggcggcggaa
    22021 ctgctgccgg acaatgatcc gctcacggcg aaagcactgt ttctcggtgg cacctatctg
    22081 aaagcacggt atccaaaaga ggcggatttc ttttacaaag cactgatccg gcgcaatccg
    22141 aatctgaaaa ttgcaaagca ggcggacgag ctcaagtggt ttcccgagaa ttttaccgat
    22201 gaaatcctgt atacgccccg cccgccggaa gggctgcgca agcgggacgt ggcaatcctt
    22261 gttgcgaagg gcgcccttat cgtccttgct gtcgggttgc tgctctgggg tctgaaaagg
    22321 cgctgcgact gagggctcag ccgcagctgt ctgcattgtt cagcacagta aggataagtg
    22381 tccgcaaaag gcgcttacag gtatgccttc tccagccggt ggatcatggc gtgaaacgct
    22441 tcatgcttcc cggcgccgat gcgctccagc tccagcgcgt caaactgcgc tttcagccag
    22501 tcgtcttttt cggcattcag atggttgtcc gccatggcga aaagcacatt gttttctttg
    22561 ttgatatggg cgttgaggag aaaaatgtag ccttccgccg ctttggacat ggcggcaacc
    22621 gcagcactgt cgcctccttt ataggcagca accgcggctt tcagatcggc aataagtccg
    22681 cgtccttctt catgttcgga cagcatcacg ccgatgggtc cgttttcctt gggaatcccc
    22741 agtttttcaa gttcggggaa aagaaaatct tcttcctttc cgtggtggca tttatccacg
    22801 aaaacggtca gaaattcgac aataccgtcc agatcttcct gagggacctg cgtaccctgc
    22861 tgcgcttttt ttacgacggc atccaaaatt ttaagcatca gggtaattcc ttcatgctcc
    22921 tgaatcagtt ctctggtagc tttcacagcg tgttcctttc atactgcacc cgcattccgg
    22981 gatgcctgtt gtggttgtac ccggaaaaac agtggtatac aaaagattat tccactgaac
    23041 agaagtgcag tgctctgatg agagcgccga aagatacatt tcgcctgacc tgctttcgcg
    23101 taaaaaagga atacacggcc tgtgatttgc tatgatatcc ggcgtaagtc tttttcttcc
    23161 tgccccgtgt ccggggctta cacctcccga cctccggcaa atccctctga aaaactcagg
    23221 gctgtttctg tcggaattac gagtaaagga tgcctttgaa atgaacgaca cccgaaacgc
    23281 ctgctgtcct tccggaaaca gctctgatct gcctgttctt cagtttgttc ttcgcaatta
    23341 ccataacttt aatgcacgtt ctacccggga tgcgctgata gcctactggc gccttcttga
    23401 acagggcggc aaaatgttct gggctgttgc cggtgccatg tcttccgccc agctgggcat
    23461 cagtattgcc ccggctatcc gtgcgggtct gattcacggc atgtcgctta cgggcgccaa
    23521 cctcgaagag tcgctgttcc gtctggtagc gcataagcat tataaagaca taccggaata
    23581 ccgttatctg cgcaaagaag atgacacacg tctgctcaat gaacgtcttc gccgggtgac
    23641 tgacacaacc atccccgaag atgaggcttt tcgtgccgtg gagcagatta tcctgcccat
    23701 gtggaaagaa gcgaccgctt ccggtacccg ccgtttctgg catgagtatt tttacgaagt
    23761 gattcaggcc attccgtcct ctctttacga aggcgatccg gaggaatgct ggctgctggc
    23821 ggcggcgaaa gcgaatcttc ccattgtggt gcccgggcat gaagactcca ccttcggcaa
    23881 tatcttcgcc tcgtatgttc agaccggaga atgcaatgca tccatcgtca aaagcggtat
    23941 tgagtatatg acctcgctgt atagtattta tccgaagctc tccgaaggag cgggcgtagg
    24001 tttctttcag attggtggcg gcatagccgg tgatttcccc atctgcgttg taccttccat
    24061 catttatgat ctggaaaaag aagcgaaacc ctggtcctat ttctgccaga tttccgattc
    24121 aaccacttcc tacggctcct attcgggcgc tactcctaat gaaaaaatca cctgggataa
    24181 gctgaccgaa gagacaccca tgtttgtgat tgaatctgac gccaccattg ttgctccgct
    24241 gatcctgagt gcgctcatgg aatgcaaggc gaaccctgag gctgccaacg cgctgatcgc
    24301 ccgctacggg gactgatcgg aaagctatcc ttttcaattc aatacctgtg ttatgatgaa
    24361 ccggatgcag tgcggtttgc cgtgctccat ccggttttta attgcttccg cccgggagaa
    24421 aagatatgcc gatacagcgt gttttgaaga gtttcctttt tctgacgcta agttcttctt
    24481 tttggttcgc atatccggtg tgcagtgaaa cacgtccttt tctggatgcg cagaggcaca
    24541 gttttgtcct ttctttcagc gacgcagaaa aaaaagaaac attgacagca ggctgccgca
    24601 caaagctttt taatgaaagc cattacaaca ttctgcttgc ccacctgcag aaatcagaaa
    24661 ccggtcattg ggagctcttt accacgcccg gggatggtat tctccatctc tatgcgcccg
    24721 gccgtgtgcc tgatcacgtt cacaccgaaa aatcaattac tgacgggcag tggcatgaaa
    24781 ttattgcagt gctggatggg agcggcgtca ggctgattgt ggacggagaa gtggccgccg
    24841 aggctgaagc gcctgcccga acagacagtt ttctatcggg cgatcttgtc ctgggggcac
    24901 ttgtggaggg caccttcggc tgtgaaggac ttattaccga tgcttttctc ctgtcaggcg
    24961 cttccgggca tgaaggacag ctgtttctga acgaccgttt tacggaagag gcttttgcat
    25021 actggcgttt ttcttccgaa gaccccttgt gtggcgaggg gagcgaaaaa agagacggtg
    25081 ttttgcgccc tgccggagtc gttcgggtga atccatcccg ccagattccg ggggggatgt
    25141 ccgctgcttt acaggaactg cctccgcttg aggatacggc accattgcgc cacagccttg
    25201 cccaggcggt tcaaagcctg gatctggctt ttctcgggga gcctgatgtc agcgatggcg
    25261 tgttccggca gtggagccat gattatgact ggatgggtaa aaaagagtac ccggagtcgc
    25321 ggccgggcgg tcccggagaa gagaaactca gacgggaagt cttcgaccct gaagcactgc
    25381 ctgtgccggg agaatcaccg cttcaggcgg tgctgcggag aacccgtgct ctgctcgatc
    25441 ttatgggaag cactgttgat ctggcggaag aagaaaatgc ctggaaccgt cttcatcaga
    25501 ttgcggaaaa agttgcaaag cagtctgatt ctcaggggaa gaaagggctt tatcttgcgg
    25561 cgtgtgcgct gcgtcgccgg atcatgctga aaaacccgct tctcgacttt gatgaaattc
    25621 tctgcgtagc cagagggact tttgaaggct ctgtccgaag caatccgaca acgcttgatg
    25681 tgcagggcgg gcactttgtc acgcaatatt tcggattcaa tgccctgcct ggcggcggac
    25741 tttacctgat ccggaatttt aaaggaaagc cggagattgt ggatcttgtt cagcatgctg
    25801 tcgtggaaga aggcgccata aaagggcaga atctggagca tggagccttt gcaacgcccg
    25861 agctttcctg ggacggagag acgatctttt ttgcctggac cgccaatccg gatcaccaat
    25921 ggatttattc aaaagaacgg tgttttcatc tgttcaggat caataaagat ggttcggggc
    25981 tggttcagct tacagacggc gcctataatg attttgatcc ctgccagatg ccggatgggc
    26041 gcatcgcttt tgtttccgag cggcgcggcg gctatatccg ctgcttcgat gcatatctga
    26101 aagtacgcag ttacacactt ttctccatga acgcccgcgg agaagagatg cacccgctca
    26161 gttatttcga gaccagcgaa tggaaccctt ccgtgaataa tgaagggcag ttggttttta
    26221 cacgatggga ttatgtggat cgggaaaact gtcttggtac ccgattctgg atcagcggtc
    26281 ccgacggaag cgatccccgg gcgcctcatg ggaattatcc tcggccgtac cataccttcc
    26341 cggatcacga accctggaaa ggggagaccg gtcatgaacg ggacagccgc ttcggcgcgc
    26401 cgctggttga aatggggatt cgtgccatcc cggactcttc ccgctatatc tttacggcgg
    26461 caccgcacca cggctcagtt tacgggacgc tctgcacgct cgaccttcgg catgaagatg
    26521 acgggcacat gtcccagatc aggcggatta cgccggagga aagctttcct gaaacggaaa
    26581 tgcccggcag aaggcattac cgctacggaa ccccctggcc tttgagcgaa gacttatatc
    26641 tctgcaacga gtgggagaat cttgtcctcc tcgaccggtt cggcaataaa gagctgctgt
    26701 gcgaccttcc ctcgctgccc tgtgccattg acgagcgtct gcgcattatt gatcctgtac
    26761 cggtgcggcc ccgtcctgta cccgccacac gtcccgtgct gccagcgaaa gagcaggggg
    26821 atcctgccac tattgcggtg atggatgtgt acaacagcga tctgcctttt cctgaaaata
    26881 ccgaaattaa atggctgcgt gtggtacaga acattcctaa gcccaatcat gccatgggcg
    26941 agcccatggt cggctatgaa cgcgaaaata cgccccgtgt gcctctcggt atcgtgccgg
    27001 tggaagcaga cggcagtgcc tatttcgagg cgcccgtggc aaaacaactg atctttcagg
    27061 cactggatga gaatttcatg gcagtgcagt ccatgcgctc ttccgccttc gtgcatcccg
    27121 gtgaacagat tacctgtctg ggctgccatg aatcaccgca tcgggccgca aaccgtacca
    27181 cctcacctct cgccatgcgc cgcgcgccct cccggctgga gccggagtgc gggcaggtgg
    27241 aaccgataag ttattaccgt cagattcgac cgatttttga aaaccgatgc gtgccctgtc
    27301 atatcaaaga ggatgcggaa cccaaagaca tgtcttatga ggctctgaaa gaagagtaca
    27361 ccttctggtt ttcaggtgcc atgttcggtg acatgaccac agcatacagc ggggtgcatg
    27421 gcggatcccg aacgatcccc ggacgcttcg gcgcccgtgc ctccaaaatc gggcaggcac
    27481 tgctggatgc cgtccattgc gacgttgttt ctgaagaaga ccgccgccgg attattcagt
    27541 ggctcgactg taattcattg cggctggctg catttagccg ggaagaagaa caggtggcgg
    27601 gggcgctggt ctggccggaa ctggatgtgg atcccgaaaa ccccactgga gtagaaggca
    27661 gagggcgccc gcttcggcac cacttctggc atgaaaatct gtggggacct tttcctgtgc
    27721 tgatcaccga tgctgaaaaa gagtgcatcc ggctgctgga cgcacagaat aaccttcagt
    27781 gggaacaagc ccttgccgac cccgtatctg ctcatatgct gccggacggg catattcttg
    27841 ctgccagtcg ccagggagta caggaactga cccggcagca tgaacaatgc tggcaattca
    27901 cagtggactc cggcgatgaa gtggtatcct gccagcctct tccggatgac aggatcctgc
    27961 tgggtgttgc cggcgagaac cggctgatgc tgctgaacag acaaggcgtg gtggaaaaaa
    28021 ctgtgatgct tcctccggcg gagagccggg atgtccggct gcgctgttgt accgtagtgc
    28081 aggaaaatct ctatctggtt tcttttgacg gagaaaaacg tatctgcggt ttaagcgata
    28141 cgggtcaatg ccggctggat cttttgtgtg aaggcgcacc agttgcattg gtcgggttga
    28201 agaacggtca ttttcttgtg ttgaccgaac agactatttg tgaatatgac cgtcagggtc
    28261 attacatctg ggaagcattg gggcctgatt ttgccgatat aaaaatcagg gctttctcgt
    28321 ctgtctgcgt tctggaaaac ggtgatctgc tgattaccaa tcagggcgct gacagggcag
    28381 agggagtatc gctctttgaa atgacccggg atcaccgtgt ggtgtggcag ctgaatcgcc
    28441 gggacgcggg tgatgcagtg cattgccagg ctttatttga tgataatttg tggatgcccc
    28501 gcccggcctt gtgaatgtca gcccagtcca ttcaatgttg tcaggttttc ccactgcgtc
    28561 tgcagaaggg agagggcttc ttccagcgac aaagcggcca gctgtccatt ttctttccgg
    28621 acaataccca gcctcaacac tttgtcggca gcatcgtcaa tatcaaaatt cactacacag
    28681 ccccaacgat tgaggaacca gtcttcaatt tttttggcgg tctcctgaac agttagcggg
    28741 cgctctgctg ttaaaaggaa ataatagccc aacagggctt ccttgcattc ctcttcttcc
    28801 gcatcatcag cgatacgatg aatcacgccg atattgttgt cgaggttctt gaaatacaaa
    28861 tgcctgatca gcgtttgcag aaagcggagc ttcctgtttt tgaaggcatt gaactgccgc
    28921 cataaatagg tgcccagcgt cagcagtccc gcaaagatta ccgccatggt cgtcttgttc
    28981 cactccactg gctggctgct cagtcccagc cagaaagcca gcagtgagcc cagtacgaag
    29041 agcgtggtac tcagccgtgt cagcaccatg atactgcccg tcacggcggc gggaataccg
    29101 aaaagaagtt tgtccatgat ccgcatgcct acacggctgt tcgggaaaag catttccaga
    29161 tccgggcggg gcacattctg gaaaagcttc aacagtgccg tgccacccgc tgcttcagga
    29221 ttgattttct gggtgctgtc cagtgttttc ttccaacgga tatacatgac gacgcggtca
    29281 taatttatga aggagatgct tttggagcgc aggcccataa acacaggcaa acactcttct
    29341 ttgactgtgg cgccacggta aaagaagtga tactcagaaa aactgttgaa atccacctgc
    29401 aggcggatgg taaagagcgc gtcttcagaa aaggcgcgtt gaatatcttc ttcaggaatg
    29461 gctttgtaat tagctttctc aagcagatgc tgaaaaagcg aataaaactc acggacatgt
    29521 ccggcttcct cacctgtctc aaagggagac gggcggaaat cagcattcat gggcgcatag
    29581 gcactcttga gtgcttcgat tttctgatga aattcaaaat ggatgagatg tgtcagctgt
    29641 gttgccagct gacggaaacg cttttcctgt cccgcaagtt tcccctgatc cagacacata
    29701 tcaataatgt cacggcggct gtaaggaacg aagaataaat cttcctctgc ctgaagcgct
    29761 gcctgcttca atgccggatc atctttttgt ctgaagaata gcatggcgga cctcctcttt
    29821 acccgaagaa tttctcttgc attctaacag agcctcaccg ccggataaaa agacttcagg
    29881 aaaactgggg ctccttcatg atccggaagg gggaatggaa gcgctttata gcatcggtgt
    29941 tgatcggtat ctgtttgtgc ttctaaaaga cctgacgctg tttccgaatc atggtcagga
    30001 gtgggcctgt atccgtcgtg tgcccagatg gaagatgggg ttcccggagc gtgcgcctgt
    30061 tgccggactc cgcacgttct tttctgacgc cttccgtatg tccccgtgac cttttttcgt
    30121 gcttcactat ctttaatttg tctattggga ctcccttttg ttattttgac ggctagatca
    30181 ggagaaccgc catggacatt aacgaacttt ttgatctcgt gcaccgggag aatgcttccg
    30241 atctgctgat cagtgccggt gcccctccga tcctccgtat ccatgggcag ctctaccggc
    30301 atcctgccga cgcgcttaac gctaccgagg cacaggagct tgtttatgcg tttctgacac
    30361 cggggcagcg ggagcgcttt gaaagagaca aggaactgga cttttctttt gaggcggagg
    30421 ggaagcgccg tttccgcgtc aatgtctatt accagaaagg ggcggttaca gcggcacttc
    30481 gtccgattcc cgaaattatc ccggcattgg aagggctggg cttgccggac agtctgcgca
    30541 atctggcgca ggcacggcag gggcttgtac tggtgaccgg accgaccggc agcgggaaaa
    30601 ccaccacgca ggcggcattt attgatctta tcaatagtac gcgcgcctgc catattgtga
    30661 cggtggaaga cccgatcgag tatgtgcatc ggcaccggaa cagcattgta gatcaacggg
    30721 aagtaggcgg cgatacgcac agctttgcga acgcgctgaa atatgtgctg cgccagaatc
    30781 cggatgtgat tctcatcggt gagatgcgtg atctggaaac aattcaggca gcgctcacag
    30841 cggcagaaac ggggcatctg gtttttgcca cactgcatac gaacgatgcc atgcagacgg
    30901 tggatcgcat tgtggacgtg tttcccgcca ttcagcagca gcagatccgt taccagcttt
    30961 ctctgacgct ccttgccatt atttcccagc atcttctggc acgcaagaac ggagaagggc
    31021 gtgtgctcgc atacgaaatt ctgcgtgcga atcaggcgat tgcgcacctg atccgggaga
    31081 ataaaacgcc ccaactgtac accattctgg agacgggtat gaaggcgggg atgatcacca
    31141 tggatcggtc ggtgaaagat ctgtatgagg ctggaaaaat cagttatgaa gaggcggtaa
    31201 ccattatgcg taacccccgt gaactgcagg agagatagcc gctcctgcat ggttcacact
    31261 attctctgac gtttcaggac acaactggaa aggaacagaa ctgtgggact tctttttgct
    31321 ctgttgacag tcgccgtgag tgctgcggat ccttctgtct gtaatggtca tgcccttctc
    31381 tatgacagcc cggcggaaac ctgggatgaa gcctttcccc tgggcaacgg cattctcggt
    31441 gcgctcgtct ggggcgacgg caatccgctg aatatttcac tggaccgcac cgacctgtgg
    31501 gatctccggc ccgtacctga atactacgag tccgattatt cctatacgac catgcgtcaa
    31561 tgggaaaaag aaaagcggta caaagaactg atcgcaaaat atgacgagcc ctatcaccgg
    31621 cccgcaccga ccaaaatacc ggctggcaga atcaccctgc attttcccgg aggaacttcc
    31681 tttgttgagg gcaggctccc gctggatcgt gcctgtgcgg aagtgcggct caaaaacgga
    31741 gccattgtgc gcacctggat tcatgcacgg gaggcagtcg ggctgattga agtggagcat
    31801 ggtgcccagg cggagtttgt cctgaatgcg ccgccttttg cgggagtgga agatcagcgt
    31861 ccccgggtag atgtggtctc cggcgagctt tcccagctag gctatccggc accggtcgag
    31921 accaaaggtg aaaactttac tgccttccag cagaccggat ggggcggttt tacttttgcg
    31981 gtctatctgt gccacaaaga acatgcagac gggcggctgg cggcatggag cattgcgtcg
    32041 tccagagaaa gcgatgatcc ttttgcactg gcaaagagcc gggctgaggc agcgttgaat
    32101 cagctggacg cgctgcgcag ttcccatgaa gcctggtggc gcacttactg gcagaagggg
    32161 caggtgagcc tgcccaacat acgcctggag cggcagtggt atctggagat gtacaaattc
    32221 ggcgcggctg cccgccctga tacgccgccc atcacgcttc agggaccctg gacggcggac
    32281 gatggaaagc tgccgccctg gaaaggtgat tatcatcacg acctcaatac gcagctgagc
    32341 tattggccct gttacagcgg caaccggctg gaagaggggt acggctttct ggagtggctc
    32401 tggcagaaca agcctcaggc ggaagactgg acacgccgct tttttgacat gccggggctg
    32461 aacgttgcaa tgaccaccga tctggaactg cggcagatgg gcggctggcg acagtatacc
    32521 cattcgtcca caactgccgc ctggcttgcg caccattttt atctgcactg gtgctacagt
    32581 caggatcgga agtttctgga agagcgcgcc tggccctggc tgtccgaatg cgccgtattt
    32641 cttgaagctg tgaccgatga aagagacagc gaaggaaaaa gaacccttcc gctcagttct
    32701 tctccggaag tgaatgataa taagcctaat gcctggttcc cctccatcac gaattacgac
    32761 aatgcgctga tccggtggac ttttgaaaaa acagcggaac ttgccgatgc actgggaaag
    32821 gcagaggagg cggcgcattg gcggaactgt ctgggagaga tgccgcctct tgcgatgaac
    32881 agccgcggtg cgcttctggt ggcgcaggac tatccgctcc gggaatcgca caggcatttt
    32941 tctcatgcca tggcaattca tccgcttggg attatcgaca gaagccaggg acccgatgcg
    33001 gaacgtatca tacgcgccac cttgcgtgat ctggacaaac tgggcaccaa actctggaca
    33061 ggctacagtt tttcctggct tggcagtctt caggcgcgtg ccggagacgg cgccgccgct
    33121 gaaaaagccc tggaaatatt cagtgaagct tttgttctcc gcaacagttt tcactgtaat
    33181 ggcgatcaga ctgataaagg ctacagcaat ctgcggtacc ggcctttcac attggagggc
    33241 aatttcgcgt ttgccgcatc cgtcaatgaa atgctgcttc agagtcagtc cggtgtgatc
    33301 gagctgttcc ctgccgtgcc ggaatcctgg gacaatgtat cctttgatac ccttcgtgcg
    33361 gaaggagctt tccttgtttc cgctgtccgg gaaggcgggc aggtgatacg ggcagagatc
    33421 atttcagaaa caggtgctgc gccggtgatc cgaaatccct ggaccggagc ggttggcaca
    33481 ccggatgaga cctcacaaga cgggcggaca aaggttgaaa gagacggtga tcgctgggtt
    33541 cttactccgg cagaccgttc ataaaatttg cctgatccat tttttaaggt gaggacaatg
    33601 cttcccgctg ccagccatct ctgtaaaccc agggatagac ggaaaactgt gcccctgccg
    33661 cctgtatttc cgtaaacccg ccgggcctgt cctttttcgc atagaagagg gcacgggtcg
    33721 tcacctgttc cagattcgga tgctccgggt cacgggtgtg gctgaataca tgcacgtgcc
    33781 cggcgagata cagtaaaggc tgttctgaac cgccaagtat ccgccgaaga gttgttccgt
    33841 tgcccagccc tctgttaagc cccaggtgat acgccgctgt ctgcggaagg aagggatagt
    33901 gcgccagtac gatcatcggc tggctctgct gctcggaaag gcatgtctgc agggatgtgg
    33961 cggcatcctg ctggaaagac cctcgtgaac tcagcagatt cggtcgtaca gtagggatgc
    34021 gcagcagagg aacgtcgccc ggcaggcggt caatagcagc tgtgtcctcg tcaggcagcc
    34081 aatactggca gtatttctcc agccgttcct ttctctttga ctcaaaagtg tatacatcat
    34141 gattgcctgc caccagataa acaggagcca acatggtcag cctgcctaaa aaatccgctg
    34201 cctgcctgaa ttcttcctct gttgctgtgg tggttaagtc accgccgatg aggaaagcgt
    34261 cgatgtccat ttcctgaaat aacgtaatca gcccggctgc acgctctgtt tttatctggt
    34321 ggcgcctgcg aatcagcaga ttaagcgtgc ccagtgcccg tttgttcagc atccgcagcg
    34381 ggttgcaaac caggctccag tagtgcagat cggttaaatg gatatatcgt gccatgaaaa
    34441 aactccgttg acccagaagc agtatgataa cgcagggcat gttgaaaaag gaaagcgatt
    34501 ttccctgtct tgagtggttg tgcacagcaa agcggatttt atacttttaa aattctaaga
    34561 atgccggctg aaacaggcgg ttttcagtga ataaaagcct tgatttaagc gcttccggtt
    34621 catgtttcct cgagaatctt gacaatagcc cttgacgtgg gaatagattt ggtgtagcat
    34681 aaggtttacc taacgaccgt taagtatagg tctggaaacc caaattgcga gtcaaccaat
    34741 aaggaggaat catgcgatga gggatattaa acgtgtcgct gtgctcgggt caggggtcat
    34801 gggtgccgct attgccgccc acctcgccaa ctgcggaatt cccagcctga tgctggatat
    34861 cgttccgccg aatctgtcgg acgaagacag aaaaagcaag aagaatcgtg atgcaattgc
    34921 cgcaggcgcc aaaggggcgc tcttgaaggc gaagccgtcg ccgctctacg tcaagtccta
    34981 tatggacatg atcgagatcg gtaacttcga agatgatatg gcaaaaattt ctgattgcga
    35041 tatgatcatt gaagtggtga ttgagcggct cgacatcaag aagaagatct ttgcgcaggt
    35101 ggctgagcac cgtaaaccgg gcagcatcgt cagctccaac acaagcggta ttctgatttc
    35161 tgctatggct gaagatatgc cggatgaaat ggcacgtcac ttcctgggta cccatttctt
    35221 caatcctccc cgttacctta aactgctcga actgatcccg acagacaaaa cagatccgga
    35281 agtggtgaaa agcgttgcgg aattcggcga aaacgttctg ggcaaaggca ttgtctatgc
    35341 gaaggacacg ccgaactttg ttgccaaccg catcattacc ttcgccatgc agtatctgat
    35401 gcatgaaatg gtcaatgccg gactgagcgt ggaagaagtg gatgcactga caggtcctgc
    35461 catcggacat gccagctcgg ccaccttccg caccgccgat ctggttggac tggatacgct
    35521 gcagaaagtg gtgggcaatg tctatcacgg ctgccccaac gacgagcgcc atgacatgat
    35581 gaccgggcct gcctggtttg acgccatgat cgaaaaaggg tatttcggca ataagtccgg
    35641 ctccggtttt tataaatcaa caaaagagcg ggacgaaaaa ggaaaacgga ttattctggg
    35701 ttacgacccg gcgacgggtg aataccgggc gccgatcaag ccccgcttcg cctgcacggg
    35761 cgcagtccgg agtgccggtt ccatggctga gaaactgaaa attatgcatt tcagtgaaga
    35821 tccgggatct gcattcctgt tcaaattctt cgccaatatg gtgcagtatg caggcaacag
    35881 aattcctgaa atcacggatg atatcgtgaa tctggataat gcctgtaaat ggggcttcgc
    35941 ctgggaagtg ggtctttttg agacctggga tcttctcggc tttgacgaag tctgcagccg
    36001 gatggaagcg gaaggtattg agttgccgcc gattgcgaaa gcgctgaaag aagcgggcgg
    36061 aaactctttc tataaaacag aaaatggtac agatcttttc tttgatctgg caagccgcag
    36121 ctataagcct gttccgtcca atcccaatga actgaaactg gtgaatgtcc gtgcacgcgc
    36181 tacggctgta gttgaagcgc tggacgaggc cagtctgata gatcttggtg acggtattct
    36241 ctgcgctgaa ttccattgca agatgaatgc catcggcccg gatattgtgg cggtgctgaa
    36301 caaaggcgtg gatcttctgg aggccggaca gtttgaaggc atggtgatcg gcaatcaggg
    36361 accacacttc tgcgccggtg ccaatctgat gctggtgctt ggtgatgcca tgcaggagaa
    36421 ctgggaagga ctggaaaaca tggtccgcgg tctgcagggt atcggcatgc gcatgaaata
    36481 ctgcaaggcg cctgtggttg ccgcgccgca ccattacacc ttcggcggcg gcgttgagat
    36541 ctgccagcat gccgacaggg ttgtccttgc cggagaaacc tatgccgggc tggttgaagt
    36601 cggcgtgggc gtgattcctg ccggcggcgg cacgacagaa atggtcgtcc gtgcttttga
    36661 atatatgccc gaaaatattc aacccaacga tgtattccct ttcatccgcc gtgctttcga
    36721 aacgatcggt acagccaaag tgggtacgag cggcgcagaa gtggtggagt ttggctattt
    36781 ccgcagcagc gacatcgtgc tgccgaatta tgatcaccag atccagaaag cgaagaatgt
    36841 gtgccgcggt cttgttattt ccgggtatac gccaccgcgc aagccccggc tttatgcatt
    36901 gggcgcgagc ggtaaagcgg cctttgaagc ggcggtctgg ggtatgcagg aagcaggctg
    36961 ggcgagcgag catgaccgcc tgatcagcac acatcttgcc aatattctct gtggtggtga
    37021 ccgccttgcc ggaacgccta tgaccgagca ggacttcctg gaccttgagt gcgaggcctt
    37081 tgtcagcctt tgcggtactg agaagacaca ggcaagaatt cagaacatgc ttgctaccgg
    37141 caagcctttg cgtaactaag caagtcagga ggaaaattaa catggaaaat gtatatgtag
    37201 tttccgcggt tcgtaccgct gtgggccggt cgaaagccgg tagtgccatt gcccatgtac
    37261 gccctgacga tctggcggca gcggcgattg gcgaagccgt aaagcgttca ggcattccgg
    37321 cggagaggat tcaggactgt gtgatcggct gcgcttttcc tgaagcggaa agcggcatga
    37381 atgtcggtcg tatggcattg ctgacagcgg ggctgcccga caccatttcc ggtgaaacgg
    37441 tcaaccgttt ctgctcttcc ggtctggaaa ccatggcgat tgcaggtgcc aaaattgaag
    37501 ccggtatgtt ggatgttgct atcgcaggcg gcacagaatc catgagcctg attcccatgg
    37561 gcggcaacaa ggtcatgccg aatcccacac tggtcagcat caacccccgt gcttatacaa
    37621 gcatgggcca gtgtgcggat aatgtaggca aagactttaa tatcacccgg gaggaatgcg
    37681 atcaatgggc gctggaaagc aacctgcgtg ccgcagccgc cattgctgag ggccgtttta
    37741 aagatgagat cgttccgctg gaggttatgg gaccgggcgg caaaacgtac gtctttgata
    37801 cagacgaagg gccgcgtccc gacacaacta tagaagggct cgcgaaactg aaacctgcct
    37861 ttgcagggcc tgccggaaaa ggcattgcca cagcaggcaa ttccagccag acgagctgcg
    37921 gtgctgccgc cacagtgctg gcaagtaaag cagttgtaga cgaactggga ctcaagcctc
    37981 tggcacgcct tcgcggttat gccgtggctg caggtgaccc gggttatctc ggtccggcac
    38041 aggttcccgc cattaaagag gcagcacggc aggcgggtat tgacatcagc gacatcgggc
    38101 ttgtggaatg caatgaagcc ttcgcatcgc agacgattta tgtggtgaaa gaactcgggc
    38161 tggatcatgc cattgtcaat gtgaacggcg gtgccattgc gctgggtcac ccgctgggag
    38221 ccactggcgc aaagcttgca acgcagctga tttatgaaat gaagcgccgc ggc
//
LOCUS       DAOPWB010000011        37283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1064_length_37283_cov_5.268696, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000011 DAOPWB010000000
VERSION     DAOPWB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 37283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37283
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1064_length_37283_cov_5.268696"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..916)
                     /locus_tag="PKN92_02175"
     CDS             complement(368..916)
                     /locus_tag="PKN92_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47631.1"
                     /translation="MCRLFFLLCVGLLASVPAFSLQPPVVLTPSDGSVISGPELTVRW
                     QPLPEGQWCQIQLAADADFSNIIANADKITGTEWRYSALPQDGKRYHWRIRARQLSDN
                     PDEGEGENSVEGESPDPDNPLPGEGEGSPEGEEGTAKPSDKNGGCGSKSATLLKELLG
                     DWLLVGLALMGLGSTAAFRSCR"
     gene            complement(949..1308)
                     /locus_tag="PKN92_02180"
     CDS             complement(949..1308)
                     /locus_tag="PKN92_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47632.1"
                     /translation="MMKRAFIFCLALALFIAITGGYGQIINSQWIAKSFISADQTSGQ
                     NPTLEVGKSYEFTLVTGSVLEQRVLSEYVLIEQNGKWVTFKKEYREPTDPYYNKDLYI
                     LINLDQVVEITYSEKVK"
     gene            1519..2862
                     /locus_tag="PKN92_02185"
     CDS             1519..2862
                     /locus_tag="PKN92_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47633.1"
                     /translation="MKQKSHVSRRNFMKSAAFGAGLMVLPSGTLFGQNTPNNKLNIAL
                     IGAWGRAEAHYEGLVRENVVALCDVDENHLAVAAKHFPQAKHYVDWRKCLDQKDIDAV
                     VCCTPDSTHAFIALWSLNRDCHVYMEKPLGLSVEEIRLVREAWLKKKGKLAVQHGTQR
                     HAKPNFDRIKELVQDGAIGELESVSTWGDRQLRRPGYPAASGNPPSTLHYDLWIGPSP
                     YHPYSPEYFSGDPGANCLQWNMYWDFGAGQVADMGSHTMDIAWNAIDASLPTSAYAFG
                     EEYNPEVTPVELTMQFEVPANSWRPAIEVAWYQGGAMPQSPLSPVDLNKIHHGALFKG
                     SKGHLVCSFDDRILIPADDSSDMTYYTPRPKEKVASPMGNFQQEWINACKGDCKTTCD
                     FEYAGNKMEMMMLGLAAYRAGEKLEYDGAAGRITNNEAANELLKRSYRPGWDLIG"
     gene            2896..3891
                     /locus_tag="PKN92_02190"
     CDS             2896..3891
                     /locus_tag="PKN92_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ47634.1"
                     /translation="MKRRDFLGTSALVGAGSLMQRGQAEESAKTVETARGSFSLTGYR
                     AAVYSPAVAEPFRLFQITDSHLALDDARGEAWRDNSKRMAAAFQNNVHFEDGKTYPNP
                     EIFEMTLARAQQEKADFIALTGDIFSFPSEAAVEFALDKLNAAGIPFGYVAGNHDWHY
                     EGLPGSSKELRDTWTARRLAPLYQGVHPLYHSQEHKGIRFVFIDNSIYEILPEQLDFF
                     RSEAATGNPLVLLMHIPLYIPGRGVGFGCGHPQWGAATDHIWELERREIWRKEGHTET
                     TFAFHKEVFSTPNLLFVLAGHIHQASLDVKQDIPQIVTAHNACGHFGVVDFKALP"
     gene            3908..5242
                     /locus_tag="PKN92_02195"
     CDS             3908..5242
                     /locus_tag="PKN92_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47635.1"
                     /translation="MMRIFILSVFAVASVILSGMACADEAVVETECDIFTLLPPENGS
                     GPLWSYGCTQVARLGDDVFVSIMETGEGVPLLSNTRWRLMRISGGEQKIVAEADGYRQ
                     REPASLAAVPPDHLFMYVNDSQEAPGVKYGHCRPHLLSFSGEKYLQKALYPKWGKKHH
                     FTDHSYRGYAADAASGHLLMLNIDATTSVQNACLMNSKGKTLATGSIVFPVRACYPQV
                     AVCGKAVQVLAVGDIVEPVDAWREYKFEQTKQQWDYVFRRLFLVGTPDLSRENFGVVL
                     EIDSVDDTGGHITNQDLWVSPEGEAWVLYSRREVQNELMRDKFFPDRSVIDSLWLVRV
                     RNGEITHKELLVEGTEESRPTQARFQETPDGALHVILYLTGADAGNKALRVFPETKER
                     VVLPLPLQEPLPSFCLANTRSGNAPSYMVDFYGTPGNNRFRYAGVRLPQSEK"
     gene            5291..5953
                     /locus_tag="PKN92_02200"
     CDS             5291..5953
                     /locus_tag="PKN92_02200"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNZ47636.1"
                     /translation="MKKDPPRSYHPTSEKPVGKKPKRVYDKVDWKPGTLLNPVPVVLV
                     ASIDGKGKPNLCTVAWAGTVCSRPPMISISLRPERYSHDLIAASGEFTVNIPSVEQMH
                     ATDYCGVVSGRDVDKFAETGLTPAPSTTIKTPIVRECPISLECRTTKKMLLGTHTLFL
                     AEIKAVQVTEALLTPSGRLALEKARLAAYAHGNYYALGKKLGFFGYSVQKKKKRKTAT
                     GK"
     gene            6034..7467
                     /locus_tag="PKN92_02205"
     CDS             6034..7467
                     /locus_tag="PKN92_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47637.1"
                     /translation="MSMSSLTRRTFLATATTTMAFGAVTMKDGKEVIEAPPVTPNTAK
                     VIPRKLSPNEKVNLAAIGAGGKGYSDLMSCKRNGANVVAIADVDWDRCAEAIYRLPDA
                     KKFKDFRIMLETMPEIDAVTISAPDHIHAPAAYMAMMMGKHCYVQKPLTHTVAEARLL
                     KNTAEECGVMTQMGNQGHCGNGVRTLCEMISSGAIGDVREVHVWTHRPVWDNQGMDVP
                     LPPEVAPENLDWDRWIGPAPWRPYHHKLAPHDWRAWQDFGGGSLGDMACHIMDPAYMA
                     LNLVEASDYTVEVVMQKGRNEQTFPIMSTIKYSFPARGDMPPVDVYWYDGHEPDPTTG
                     EEVYNRPQRPASVPLDEVLGDKEFNGSFFVGDKGILTAGEYGDNPRLMPAAAMEDYKF
                     PDETLERIPREDPYFDWLRGITEGKMPCSNFSYAGPFTEMVNFGNLVVKSGQKLHWDN
                     KKGEVMGVPNPGEIVSKEYRKGWELPC"
     gene            complement(7554..9437)
                     /locus_tag="PKN92_02210"
     CDS             complement(7554..9437)
                     /locus_tag="PKN92_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ47638.1"
                     /translation="MRNIFFTLLLMLLAVRGSAADTDPVSVLQSNASMEAKIEACRLI
                     AASGDESAIPVLEPLLNDSALSHMARYALEPMTGEAADQALLRALEKASGTVKAGIIA
                     SLGVRKTESAFDALAAAVDDPDNAVAEAAVRALGRLGTAAAAGVLETFSLRNDLPVAL
                     REATGEAFMDTAEAFLARDREDHASGFYEYVYFKKSFPVHVRAAALRGAVRAHETDAG
                     WKLLVKAFQSDESALFEMALRIALEITPRARTAAKLAALLPEYSPERKLLVIQTLGEL
                     GVTKAGHALIPEAMAGRNDLRIAALNALTRLKYEAALPLITSLAVSADEELAPAARDC
                     LAFFPGEAGDQILERLLKSEDSDTRLAAVGMIALGGLPDASNRLLNIARRDSAMPVRL
                     AALEGAARHGGIGHLSEFMRHLTRPQSKEERTAAEDALRSIITRSRDKGIPDGIVKDL
                     INTLNRAAAPQQPALITILTATGSQRAFDNLFEKTGADDEALRDAAVKAITDWPTLIA
                     LPTLLDWVSNNTAQRTAAFRGAARLLAAKQLGSEEALTHYRQLLTLVQNTDEKKLLLS
                     GMGNLDSPDALLLVLDLLSDDQVKAEAVQAAMAIVRRLGETPDSEAIKEKAYALIPEL
                     KKD"
     gene            complement(9509..10807)
                     /locus_tag="PKN92_02215"
     CDS             complement(9509..10807)
                     /locus_tag="PKN92_02215"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47639.1"
                     /translation="MSKKLSRRRFLACAAGALAAPMIIPPSALGRGGAVAPSERITAA
                     GIGIRGRGFHDLRWMIRNPDVQILAICDIQKKQRLAVKEFVDGHYGNEDCAMYSDMRE
                     FFAERPDIDAVLIATGDHWHAQASIRAMRAGKDVYTEKPSAMTIAEGRAVVETAAKYG
                     RVYQTGTQRLSEANHVFCIEMARTGRLGNVATAYAHIAPWDAAKMPRKWLPAQPEPPK
                     DEVDWDAWLGPCPWRPYNEEYVLGGWRGDYDFHTSCIGEWGAHTFAQAQMGLGLGETS
                     PVKYEYVDNDSGDGMVCHFSTGQKLIFSRGDKYWYGSCGELFEGERGWVAAADGYSVP
                     DVSDPEMLAEFDEVVGEYKARTGRSLDHMRNFLDCVKTRQEPVANPRVMHHSMCTVHA
                     ANICMWLERDLVYDPKKECFVNDDEANRFLARAERAPWTL"
     gene            complement(10923..11828)
                     /gene="ispE"
                     /locus_tag="PKN92_02220"
     CDS             complement(10923..11828)
                     /gene="ispE"
                     /locus_tag="PKN92_02220"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNZ47640.1"
                     /translation="MSHHWRSYAKINLYLDVLRRRRDGYHNIETVFQSVSLYDELTFS
                     ELPEDIVLTCSRPELDTGSSNLICRAAALLKESTGCTKGAQIHLQKNIPLAAGLAGGS
                     GNAAAALAALNRLWQLELPPAQLLACARKLGADVSFCLSGGTQAATLKGEVLYPMPPL
                     PPVFFVLIHPDIQVSTPFIYTHPLLRRSNARPVAGFSSSFRRALRALKESRWQHLVFN
                     AMEVPAFSEYPQLAELKGQLVKQGCDAAAMSGSGPTLFGLFDLCEKAEQAIAFFRQQY
                     PSLGCTLVQPSSKGVAYFRESFSEP"
     gene            complement(11941..13269)
                     /locus_tag="PKN92_02225"
     CDS             complement(11941..13269)
                     /locus_tag="PKN92_02225"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNZ47641.1"
                     /translation="MGMHRRFVSSALFFSFFLASIFAPLYAAEINASPEVTLLREEAG
                     GKLYQVANQKMLVMEGTPEEMGFQHGLLLAKEIRHIMKEGYTNKALWDRGYSKEYVAA
                     QSERMEKFFPESVREELRGMVKGLQAAGDDGISYEDLRLGITQAEILHFPPDGGPACS
                     NFACWGKWTTDGRLLHGRNLDWNIKGDAQDDHTILVWRPKGGIPFMMVGWAGGVGSVT
                     GMNSRGITLGEMTLPSSDVTFDGLPLFLQMRLCLEQCATLEEAVQFFQKCHRTTGWNF
                     IIGDSKIPDARALETDAKYCTVFAPMDPKESDATGHWGMVDAVRRTNHPVGMEQLLRL
                     AAAFGHEFGLEISSEADIETAKPLLMTQDSWQRYDWISREIDRQPGAMDVAQAAAILG
                     SGPVQAGNTLHSCVFDPKNKSIYVANAGNNPPVPAWKLPYVLFDLSPWFD"
     gene            complement(13463..14563)
                     /locus_tag="PKN92_02230"
     CDS             complement(13463..14563)
                     /locus_tag="PKN92_02230"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNZ47642.1"
                     /translation="MKIAPLPLPMPRDTRFGKGASKLLLPLCAAFGTKGLVVHSASVK
                     KNGRLEALLSEKPGETDCWQHEGGEPALVQTEALRRYIQNTAPHWVAAIGGGSVLDLA
                     RAAAGLSQAPLSVQEYHDGAEIPESPLPFIAVPTTAGTGSEATTVSVLTNRATGVKKS
                     IRHPSFMARLVLLDPELMTGTPASVLAASGMDALTQAIEAFCSRGATCLTDALAKDAA
                     EKVFNALPGACSGDPSFFDDMMLGSYMAGLALASARLGLVHGLAHPLGARYHAAHGLV
                     CAFCLPPVLAFNRPVIPEKYDTLKRIFRRDPLEAIAALIQKLPLASPFTGQVLSDTQA
                     IIEETLASGSTKANPRDVSADQVQALLNHLFQ"
     gene            complement(14560..14925)
                     /locus_tag="PKN92_02235"
     CDS             complement(14560..14925)
                     /locus_tag="PKN92_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNZ47643.1"
                     /translation="MIRRKDDMHCEIRDNMRGGAGSVSLRHFFHADEFTAPVRLCASL
                     SIPPGAGIGSHQHLKEDEVYYITRGTGILDDGETQSRVCAGDAILTGRGESHAIRNDG
                     NETLEFIAFIACYPEEDKA"
     gene            complement(14999..17029)
                     /locus_tag="PKN92_02240"
     CDS             complement(14999..17029)
                     /locus_tag="PKN92_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HNZ47644.1"
                     /translation="MNTNTTDTVKQTLFVDGRELPINGERNVLEMARKAGIDIPTFCY
                     HSELSVYGACRLCLVEIEGRGIQASCSVAPEPGMRLRTNTKKVRDIRKISIELLLANH
                     DMTCPTCSRSATCRLRELAERMGIDKVRYKPIERKAPLDHSSLALVRDPNKCVLCGDC
                     VRACHELQGVGAIDFAFRGADSAVLPAFNQDLAEVECVNCGLCASVCPTGALTPRPEM
                     EEVWADLDNPNKIVVAQMAPAVRVGLGEYFGMEPGSITTGQAVAALKAIGFDKVYDTS
                     FAADMTVLEEGEEFLDRFKNQGVLPLFTSCCPAWVKYAEQYHHELLPHLSSCKSPQQM
                     FGAVARKVLPKVMDMKGKELVVVSVMPCTAKKYEAKQEKFIHDGVPEVNHVITTAELG
                     LMLRQCGLQLADLEPAAFDKHISFKTGAGVLFGVTGGVTEAVLRYAVEQVQGKPLEDV
                     DFREVRGEKGLREATVTVDGIELRLAIVNGLAQAAAVIESIKNGTCAYHLVEVMACPG
                     GCVAGAGQPHTFNPAARQMRTRAIYDNDKMLDYQKSQDNPELIKCYADHFDGVGGHTA
                     HETLHTEYTHRKRLHEIDIALQEGESKGTLPIQVCVGTNCFLNGSQDLLKKLMHHVDQ
                     QGLTDQVSITATFCMENCGKSPNVRVGATHLEACTFEAACRSLEEQLGSKAL"
     gene            complement(17026..18960)
                     /locus_tag="PKN92_02245"
     CDS             complement(17026..18960)
                     /locus_tag="PKN92_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNZ47645.1"
                     /translation="MNTSVMTDRPGLKEASDIFQQRISRVEHRIVICAGTGCVAGGAL
                     AVRDAFVRELTALGLEVATNLHADGALDSDKPISVYLSKSGCQGFCQMGPLVTLEPEG
                     ILYTRVTPEDVPEIVDVSLVKKSVVERLLYRHPQTGEQCKGKDTIPFYQRQERRVLKE
                     CGMLDPGDLYEYINHGGYEAARRALCDISDADICNEILQSGLRGRGGGGFPTGRKWDL
                     TRIQPGDQKYVICNGDEGDPGAFMDRSVMEGNPHAVIEGMVIAAKAIGAQEGYIYVRM
                     EYPLAIRRLQEAMDQAREFGALGENIFNSGFSFELHIMEGAGAFVCGEETALIASIEG
                     KRGMPMPKPPFPAQSGLWGKPTLINNVETLASVPLILRYGPEQFRAQGTEKSPGTKTF
                     ALTGHVANTGLIEVPFGATLREIVYEIGGGVTDNAGNVTDRPFKAVQIGGPSGGCLVA
                     EHLDLPLDFDSLGSVGAMVGSGGLVVMNDQTCMISVARFFMQFARNESCGKCVLCREG
                     TRQMLALLDDIIAGEGTLETLEILEQTAEAVKIGSLCGLGKTAPNPVLSTLRYFRKEY
                     EAHVIDKHCPAGVCPALCTPVIIDELCTGCGLCKRKCPVEAISGERKEVHHIDQEKCI
                     KCGVCLESCRFDAIVQGAAQ"
     gene            complement(18957..19445)
                     /locus_tag="PKN92_02250"
     CDS             complement(18957..19445)
                     /locus_tag="PKN92_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNZ47646.1"
                     /translation="MQQTMRKFDTLCDIIDRFDRDPAKLISILQAVQAEYRYLPKEAL
                     AYIATSLNLPPARVFGVATFYAHFALEPKGKYVIHVCDGTACHVKNSQGLVNAIQKKL
                     GLTTDKNTTEDLLFTLETVSCLGACGLAPVLVINEDVHGMMTPQKCVEALDKIIEREA
                     QQ"
     gene            complement(19472..20650)
                     /locus_tag="PKN92_02255"
     CDS             complement(19472..20650)
                     /locus_tag="PKN92_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNZ47647.1"
                     /translation="MNQDTLFLEVDFAQRRKHTEHICGDAFQSQRVDGSDRILAVLSD
                     GLGSGVKANILATMTASMALKFAASSMDFVHSAEVIMDALPVCQIRRISYSTFTIIDC
                     SAGGRARFIEMDNPPLLFIRDGKALPLERREMVSPKWQDRVLRFSDMETMPEDRVVLV
                     SDGIVQAGLGSKALPLGWRDQGLREFVLDRIRQDPVVSARTLAEDVLREALSRELNRQ
                     AHDDMSCAVLYLRKPRKMIVLTGPPFDPNHDAEFARRLQEFDGRKVICGGTTTTIVAR
                     ELGVEAKLDLSRMSEHIPPQSTMEGVDLITEGILTLTRTAQLLEKGAGVRGDDPAEKL
                     AALLLESDIIHFIVGSRINEAHQDPTLPIDLEIRRNIVKRLTHCLEERYLKETHTLCI
                     "
     gene            complement(20647..22386)
                     /locus_tag="PKN92_02260"
     CDS             complement(20647..22386)
                     /locus_tag="PKN92_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNZ47648.1"
                     /translation="MDRLFPIDTMETTCQDCYKCLRQCPLKAIRIEDGHAKITPDLCV
                     ACGICVQVCPVQAKKIRLDLGRARHLLDQEAGVYVSLAPSWAGEYPGLKAEQMICALK
                     RLGFAGVSETALGAQEVSAAVAKMLNRNGLYISSACPSTVRLVRKYLHDYAANITAVH
                     SPVMAHSKLLRQHFGDAIRIVFIGPCIAKKTEADEHRDLLDIALTFEELNDWFRQMKL
                     DPYTMTPDKNDRFVPFSADEGALYPVEGGMITTIQALGAKDTVRYVAVSGIDNICKTL
                     KGLEETSLQHPVFVEALGCEGGCIGGPCAKASGALLGKRILVEDHAIIPPLPVVREHT
                     LDITETFTPAPAAPPAPDPALVRGALRRIGKRLPEDELNCSGCGYNTCREFAAALVRG
                     TAEETMCVSHMRALAQKKANALLRSMPSGVVIVDAAMHIVECNERFARMFGPETTEIY
                     ETCPGLKGCSLAKIIDFTDMFRTVLKFDQEIHYDHFRYKNRLLNITVFSLDPHQVVGA
                     VILDVTRQEGLRDQIAQQAQEVIRRNISTVQEIACRLGEHMADTEILLRSIAEGYACD
                     SGRLAGKPKEKER"
     gene            complement(22390..22650)
                     /locus_tag="PKN92_02265"
     CDS             complement(22390..22650)
                     /locus_tag="PKN92_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNZ47649.1"
                     /translation="MNDPVTIVICMGSSCFARGNRNNLTLIENFLEKHNLTAAVRLEG
                     SHCDAECQKGPNIRIGDTLYQHVDPGMLLDILETHFAVNAKE"
     gene            complement(22647..23324)
                     /locus_tag="PKN92_02270"
     CDS             complement(22647..23324)
                     /locus_tag="PKN92_02270"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNZ47650.1"
                     /translation="MIKEETIRSIPEPTLRRVPSYCQVLLGLAEQGQEYVSCTQLSEA
                     LHIDPTLIRKDIALAGATGRPKVGYLTRDLHRQLENFLGWNNARDAILVGAGSLGSAL
                     MGYKAFDQYGVNIVMAFDTDPEKVGRKIHDVPVLPLTRMPNLLERMKVHLGILTVPAE
                     YAQTVGELMVMNGILAIWNFAPLALKVPEGIIVQNEDLFSSLGILSSKLAAVLKNEDD
                     HFKEGER"
     gene            complement(23691..24344)
                     /locus_tag="PKN92_02275"
     CDS             complement(23691..24344)
                     /locus_tag="PKN92_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47651.1"
                     /translation="MTTQFNEDLNILKAKLASLKTVWEGKPSVLELKQANYNWKQMEW
                     WAFYFEYLCYKVLLSDFQHPGGHFDSVTFDLRRNVNWDLKAKAIKSDDHRAILNDVRA
                     MKQSIAQNGGEHGVIIALCDVEYNDIDRSFQKWHTELKGDLSTYEIERKARTSISRYR
                     KTKAVLTEILFLRFTCADLELLSTMKQGRNSNGKPRPEKYMIDLENIGNLQVGHIAF"
     gene            complement(24328..25260)
                     /locus_tag="PKN92_02280"
     CDS             complement(24328..25260)
                     /locus_tag="PKN92_02280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNZ47652.1"
                     /translation="MSSPNKLTMADLSINDAVNNHLTNCIITGDCLEVLPYIKDAEVD
                     LILFSPPYDGIRTYHKGWNFDYSALGKSLYRVSKEGGVCAVVIGDGTKDFAKSLTTAR
                     LTVDWCDNAGWRLFEQCIYYRDGNPGAWWTQRFRVDHEYILLFFKGKRPKTFEKRSLM
                     IPSKHAGKVYSGTDRLTNGGFKEIVPKEVNSMKCRGTVWRYSTSNTEGNRLKLKHPAT
                     YPDKLAQDIIQCFSMPNDFVLDPMAGSGTTCVIAAKNQRRYLGIDISEEYCDIALTRL
                     NIEKNVQLSLFDLQKSKNMGVANTEDGTFNDDTI"
     gene            26272..27516
                     /locus_tag="PKN92_02285"
     CDS             26272..27516
                     /locus_tag="PKN92_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47653.1"
                     /translation="MEKKPSNLSRRDFAKASAAAGFAILASRSGQAQDSAETLKIGLI
                     GCGGRGCGAAVNCLTGNDNVKLVALADVFEDSVIKAKANLERNNDPRVKNKLAIEEEL
                     CFHGLDAYQKLLETDVDIVIHATPPYCRPMHLEAIVAAGKHLFSEKPFAVDSPGIRRC
                     LAAAKVADEKGLTFVTGLQRRYQDKYLQTIEKLQGGEIGEILYARVYWNGNLPFCHDR
                     LPDESDLAYRLRNWYNYIWTCGDNIVEQHVHNIDVINWLLGAYPVKVVSNGGRAWKPA
                     EEKYGDIWDNFACDFEYANGVHMLSMSRHWNNSKSDVWERVVGTKGKAACGDLGRDRS
                     DPYVNEHIAMLQSIRGEIDRINDGERTAYSTLTAIMGREAAYTGQEVTWEQALNCEKS
                     RVPADLDFAKSYPVGPIPVPGA"
     gene            complement(27589..29067)
                     /locus_tag="PKN92_02290"
     CDS             complement(27589..29067)
                     /locus_tag="PKN92_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47654.1"
                     /translation="MKPHEIIEIAPGYPPAEIRWYLGKTAPSRKGIFFAILLFLVGIA
                     GLFFSTLIDLAYSHEVPWRTAAIYLAPVYVPREQIAYPLSARPLDARLWTDDRGLDML
                     AGIATVPGSETAPRREMLFALDLASETLQIPLLSGRLPEPGQAEVLAGDLASPEPFTI
                     NGITFTVTGRLHPAASGFICSYLLPDRSAFAELFDNADPEVFQGVFIPENGLMIPEPP
                     SGKVRHPVASETDADELPGKETDAVIAVPAVIGGVLRTHDYAARCSFLSMLLCALGGS
                     LFFIKLFRRFHLQERRLFAPVFGAMEQHRRFFMLMHLLLYGAFFLSMYSAISNPLMGY
                     ELKRYTMDIFSDGSLEYIGSAYDKGNILQATWATFYNNYIEQTLVMTFALSLILPLGV
                     LKNIASFFIVGGAMSPLWVGTAAVLILHCLTMVVELEAYIIACFFVLLWSALLLRGIR
                     KRQVSAAIRKGSWLLFNAALLTAVILAAAALYEAVTLIHLFS"
     gene            complement(29064..30488)
                     /locus_tag="PKN92_02295"
     CDS             complement(29064..30488)
                     /locus_tag="PKN92_02295"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNZ47655.1"
                     /translation="MHIVKRILLGVVVVLLLFTTLFLVFVGPWPVYKDSRYRESAYYK
                     DALAAVDADAALSNFTDTPEALRAGWGVRIITPSIGLPIAGYGARKDGMRSQGVRDDL
                     KVKAVSFTDGKDTVVLVGVDMLIIPPNIAEMTVDKVASRIPLASRNLYFTASHTHCGP
                     GGFGPGLAAKFTGGTYNPSVPEFLSSSFADAIVEAWQNMKPAKLAHGTVDARAYIRNR
                     AREHGDVDPNLNYILVEQESGRKCYITRFSAHPTNFGSSMMQFSAEYPGALMRSLEQQ
                     TNATAVYLGGGLGSMSGKGIEADGPDARVELMGKTLADLIVAASASPKFTDRVDIASL
                     AVPVGVPPFQARPLSPKWRLSPLAAKLIGLPNEGFVQGARIGDLLFVGLPFDTSGELT
                     RQWREEKAAAGWDLWVTSFSRAYLGYLSPEKYYFETNKRGQLDYEIGFMNWLGPNSTT
                     YFKEILDRVVMQLGKSAPTLAAAP"
     gene            complement(30695..31747)
                     /locus_tag="PKN92_02300"
     CDS             complement(30695..31747)
                     /locus_tag="PKN92_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNZ47656.1"
                     /translation="MKAWQWQAPEKLRLVELPRPVPKRNEALIRVEAVGVCGSDIHYF
                     LEGAIGENRLTAPTVLGHEYAGIVEELGADADPALLGKRVAVEPGIPCRRCEWCRKGR
                     YNLCTSLFFPGGPGSDGAVCEYIAVDHEFCFPVPGTMSAGTAAMVEPAAVAVHTAELA
                     QLRPGDTAAIFGLGVIGLLTARMLQCCGAARVLAADILPYRVEAARLYGIQEVFCNRK
                     GSIKEGGASAVGWIQEMTERRGVDVAFDCTNSADGLALACAAARRGGRVVLTGISGNE
                     MDPLPVSIARRRELCLQWCRRFVHNYPATLDYIQSGKLDVDSLITHVFSFDEAPDAFA
                     LTSAYGDGVLKASIEW"
     gene            complement(31811..32149)
                     /locus_tag="PKN92_02305"
     CDS             complement(31811..32149)
                     /locus_tag="PKN92_02305"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNZ47657.1"
                     /translation="MKKSELEHIGDILNRMKKTTPLGLTLEQAQIWEHWTELVGEHLA
                     PHCRPYDIRDGELRIMTDSTVWMHKISYVKWDLLRRINRMAKKELVSDIYFMLDSDET
                     EKNGRKKRKP"
     gene            complement(32130..33209)
                     /gene="recF"
                     /locus_tag="PKN92_02310"
     CDS             complement(32130..33209)
                     /gene="recF"
                     /locus_tag="PKN92_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNZ47658.1"
                     /translation="MKLTKMTCRHFRGLSDMELIPGAGINLLYGGNAQGKTSVLEAIL
                     YAATARSHRTTNDADLVCHEKEDFHIYLEGETAKRSLSIEAHWWKGAKRFKVNGVAQN
                     RLSDLLGQLCVVFFSPDDIALIKGGAAVRRLFLDMELSQLSTPYLRALQQYRQALRQR
                     NEMLKHLQFDAELMGLWEAQLAAQGSRLMDERTTYIAQLSAIASPLYSQIVEKEPLEI
                     IYAPDIPDKEEFASLLERSRKTDQQRRSTGRGPHRDDLDILISGRSARVFASQGQQKS
                     AALIVKLAEVALVHGRLGDYPLILLDEAVAELDENRTHRLFHALPEGAQTLITTAQPA
                     QLPETIRSDSRIFRLEKGTLEKVGT"
     gene            complement(33394..37206)
                     /locus_tag="PKN92_02315"
     CDS             complement(33394..37206)
                     /locus_tag="PKN92_02315"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNZ47659.1"
                     /translation="MAQWISVLPAVLIMATLITSAHGQVVFIGSESGENNSSNSSFSF
                     NVTVPADTTLAVGIAHNGDSKTASMTLGGSPMIALPATDFGSNYERIRMFYLVNPPKN
                     TVSLATSYVDGNFKGKVAGIFYFKGNDPSEPIGKNTCEWSGDPFQAVPLAPSDLPLGC
                     NIVVGLSGYNTKFDAINRNDYVSGGGTVNQQYAKWPNWYNAGAVRYDYRPGLGEVITG
                     NIVGPVKPNWNSYSGNGVGAVAVINPVKSTITYAVTPANTGTITGGAAAIFPRTGSTS
                     FTVAANPGYSFANITTTRGTLTGTFPNYTLSNVTVNTTVTASFNAVMRNLTYGTPTGA
                     TISGPATVQDTIGTAIFSVTVQPGYQLVGAPTSTSGTVAADGANWKLTNVKTNATVNA
                     NVQPIIHTVGYSADTGGSVAGADTIQNTVGSIPVTVSTDPGYTFTGISASSGTVNGSY
                     PNFTLSNVTADTTVTANFTAVYHNITPAVSTNSPDGAAIGGTVTGLSGTIQDTIGTVN
                     FTVDVNPGYVLAGPTVSNGTISGTAPNFTLENVTADTTVTVAFTNTRPTISAIPGALT
                     LECGASYTAANARVGVTANDAEDGPINSTLIAVGGVTFPLTAVGVHTITYNVSDKAGL
                     AALQQTRTVTIKDTTPPAITLNGAAAMTLECGVDSYAEPGASATDSCEGALSVTPSGS
                     VNTDAPGVYTVTYTAEDSEGNIGTATRTVTVEDTTPPVITRIGNENIQIFTGDPYTDE
                     GATVADSCDTTVSVVATGSVNPNVAGSYTIRYNATDANGNHAVEVVRNVQVVSLIDPS
                     LVSVEVDSEFSVVVTWNQDIAYLNAALVAANYTVSGNGAGSLSTNPTRVEIAGAKAVR
                     LWWDNAGEMLNGKDIVITVDPAFTGNFGNPIDGNQSTDAGGAIGIAPVIALNGGDITL
                     ECGVDEYAEPGATANDNVDGNVAVVITGVINTGATGIYTVTYTAEDAVGNISTVTRDI
                     TVEDSTAPILTLLGDADMEAPCGYDFVDPLVTAADACDGDLTAKVVVAGDTVDTSVSG
                     AVFTITYDVTDDAGNPAASVSRTVRIADNEGPEIILTGDNPLVLDGGTAYVEYGAVAI
                     DFCGDAVYTDDIVIEGGDDVNPLVIGSYAVTYTVADADGNETVAVRTVEVKRDSCKIR
                     VTLDITPNPAVPGEKVTMTALEQSSSCHVGALHYQWEKRDNGKAGDFQPIPGADDAST
                     FVISSVDFDDAGDYRCTISDDMITVNSAAVTLSVGTGVPALSRLGVLLASAAALAAGA
                     AALRKKRD"
BASE COUNT         8481 a         9886 c        10155 g         8761 t
ORIGIN      
        1 ttaagattcc tgcctccctc cgtcattcct gcgaaggcag gaatcttaat cagggcatgg
       61 acgtcttgat ctgcacatcg ccctgatcca caccaaagac ttcaagacat acaaacacaa
      121 aataagcctt aatttaaaag acttctccgc gtagttgacg gcaacagcat gacccttata
      181 aataacatct cgcgttaaga ttcctgcctg cgcaggaatg acggagggag gcaggtatga
      241 cgagagaaag caggaatgac gccgggggat ggacaccttt gcttcacagg actgcattcc
      301 acacaaaagt cagtggacag acacagacac agacacaggc gctgctgttc agtacccggg
      361 tacagagtta ccgacagctg cggaaggcgg ctgtgctgcc cagtcccatc agcgccagcc
      421 ccacaagcag ccagtcgccg agcagttcct taagcagggt tgcacttttg cttccgcaac
      481 cgccattttt atccgatggt tttgcagtac cttcttctcc ttcaggcgat ccttcgcctt
      541 caccgggcaa gggattatcc ggatcgggac tctcgccttc aacactgttt tcgccctcgc
      601 cttcgtccgg gttgtctgac agctgacggg cgcggatacg ccagtgatag cgtttgccgt
      661 cctgaggcag tgcactgtac cgccattctg tacccgtgat tttgtcggca ttggcgataa
      721 tgttactgaa atccgcatca gcggcaagct gaatctgaca ccattgccct tccggcagtg
      781 gctgccatct gacggtcagt tccggtccgg aaatcaccga cccgtccgaa ggtgtcagca
      841 ccactggcgg ctgaagggaa aatgccggca cagaagcaag cagaccgaca cagagaagaa
      901 aaaagagacg gcacatagca aattcctcgc tcccgataag caacagatct atttaacttt
      961 ttcggaatag gttatttcga caacttgatc aagattaatc agaatgtaaa ggtctttatt
     1021 atagtaaggg tccgttggtt ctctgtattc ctttttgaaa gttacccatt ttccgttttg
     1081 ttctatcaat acatattccg ataataccct ctgctcaagt acacttccag tgactaaagt
     1141 aaactcatag gatttcccga cttccagtgt ggggttctgt cccgaagtct ggtcggcgct
     1201 gataaaggat ttagcgatcc attggctgtt gatgatttga ccgtagccgc ctgtaatggc
     1261 aataaagagg gcaagagcaa ggcagaaaat aaacgcgcgt ttcatcatgg tgcagctcct
     1321 ttttcgggtt gatgtgcaca aaaacaaata acagtgattc cactataagg aactgaagag
     1381 gaaaaagcaa ggagatcaat gcagcgatgg aggcggggaa aattccatgc tgcttttgta
     1441 tgtgtcggtt ctgtatccgg gctgcaaaat atgtataata aatttttcgg gcttgccaca
     1501 acagaaagga atgctcccat gaaacagaaa tcgcatgtca gtcgccggaa tttcatgaaa
     1561 agcgccgcct tcggtgccgg acttatggtg ctgcccagcg gcacgctctt cggacagaac
     1621 acacccaata acaaattgaa tattgcactg attggtgcct ggggcagggc tgaagcgcat
     1681 tacgaaggac ttgtccgtga aaatgtggtt gctctgtgtg atgtggacga gaatcatctg
     1741 gctgttgcgg caaagcattt tccccaggcg aagcactatg tggactggcg caagtgtctg
     1801 gatcagaaag acattgatgc agtggtctgc tgcacgccgg acagcaccca tgcttttatt
     1861 gctctgtggt ctctgaacag ggattgccac gtttacatgg aaaagccgct gggtctgagc
     1921 gtggaggaaa tccggctggt gcgcgaagcc tggctgaaga aaaaaggcaa gctcgccgtc
     1981 cagcacggaa cgcagcgcca tgccaagccc aattttgacc ggattaagga actggtgcag
     2041 gacggcgcca tcggcgaact cgagtccgtg agcacctggg gcgacaggca gctgcgtcgg
     2101 cccggctatc cggccgcatc gggcaatccg ccctccacac tgcattatga tctctggatc
     2161 ggcccgtctc cctatcatcc ctactcgccg gaatattttt cgggcgatcc cggagccaac
     2221 tgtctgcagt ggaacatgta ctgggacttc ggcgccggtc aggtggcgga catgggcagc
     2281 cataccatgg acattgcctg gaatgccatc gacgcttctc ttcccacgag cgcctatgcc
     2341 ttcggcgagg aatacaatcc tgaagtcaca ccggtggagc tgaccatgca gtttgaggtg
     2401 cctgccaaca gctggcgtcc cgccattgaa gttgcgtggt atcagggcgg cgccatgccc
     2461 caatcgccgt tgtcgcccgt tgatctcaac aaaattcatc acggtgctct gttcaaaggc
     2521 agcaaaggac atctggtctg ctcctttgac gaccgtattc ttattcctgc cgatgacagc
     2581 tccgatatga cgtattacac gccgcggccg aaggaaaaag tggcatcgcc catgggtaat
     2641 ttccagcagg aatggattaa tgcgtgcaag ggtgactgca agacaacctg cgatttcgag
     2701 tatgccggaa ataaaatgga aatgatgatg ctcggtcttg cagcgtaccg tgccggagaa
     2761 aaacttgagt atgacggtgc tgcaggcaga atcaccaaca acgaagctgc caacgaactg
     2821 cttaaacgca gctatcgccc gggctgggat ctgatcggct gaatacagcc ttctctacag
     2881 tggaaagaca gacctatgaa gagacgtgat tttctgggta cctcggcact tgtaggggcg
     2941 ggttccctta tgcagcgcgg acaggcggaa gaaagcgcaa aaaccgtgga aacagcaaga
     3001 ggcagttttt cacttaccgg ttaccgggca gcggtgtaca gccctgcggt ggcggagccg
     3061 ttcaggcttt tccagatcac cgacagccat cttgctcttg acgatgcgcg gggtgaagcc
     3121 tggcgagaca acagcaaacg catggcggca gcctttcaaa acaatgtcca ttttgaggat
     3181 ggcaagacct atcccaatcc ggaaatattt gaaatgactc tggcgcgggc tcagcaggaa
     3241 aaagctgatt ttattgcact taccggcgat atcttcagtt ttccctctga agcggcagta
     3301 gagtttgctc tggataaact gaatgcggca ggaatccctt tcggctatgt ggccggaaac
     3361 catgactggc attatgaagg gctgcccggt agctcaaaag agctgcgcga tacatggact
     3421 gcccggcggc tggcgcccct gtatcaggga gtgcaccctc tttatcacag tcaggagcat
     3481 aaaggtatcc gctttgtctt tatcgacaac tccatctatg aaattctgcc ggaacagctt
     3541 gactttttca gaagtgaagc ggcaacaggc aaccccctgg ttctgctgat gcatataccg
     3601 ttgtatattc cgggcagagg tgtaggcttc ggatgcgggc atccccaatg gggcgctgcc
     3661 accgaccata tctgggagct ggagcgtcgt gaaatctggc ggaaggaagg gcacaccgaa
     3721 acaaccttcg cctttcataa agaagtattc agcacaccca atcttctgtt tgttctggca
     3781 gggcatatcc atcaggcatc gctggatgtg aagcaggata ttcctcagat cgttacagcg
     3841 cataatgcct gcggtcattt tggtgttgtg gatttcaaag cactcccgta aggcggagaa
     3901 ggactgtatg atgcgcatat ttattctgtc agtttttgct gtcgcgtccg tgattctttc
     3961 cggcatggcg tgtgccgatg aggcagtggt agaaacggaa tgcgacatat ttaccctgct
     4021 gccgcccgaa aacggttcag gccccctctg gtcttacggc tgcacgcagg tggcacgtct
     4081 gggtgacgat gtcttcgtca gcatcatgga gaccggggag ggggttcctc tgctcagcaa
     4141 tacacggtgg cggctgatgc gtatttccgg gggagaacag aaaattgtcg ccgaggcgga
     4201 cggatacaga cagcgcgaac ctgcctcgct tgccgctgtc ccgccggatc acctttttat
     4261 gtatgtcaat gattcgcagg aagcgcccgg cgtgaaatac gggcattgtc ggccccacct
     4321 tctttctttt tccggagaaa agtatcttca gaaagcgctt tatccgaaat ggggtaagaa
     4381 gcatcatttt accgaccact cttacagagg gtatgcggcg gatgcagcct ccgggcatct
     4441 gctgatgctg aatattgatg cgacgaccag cgtgcaaaat gcctgcctca tgaacagcaa
     4501 agggaaaacg ctggcgacgg gcagcattgt gtttcccgta cgcgcctgct atccgcaggt
     4561 ggcggtttgc ggtaaagccg tgcaggtact tgccgtgggc gatattgtgg aacctgttga
     4621 tgcctggcgt gaatataaat tcgagcagac aaagcagcaa tgggattatg tgttccggcg
     4681 ccttttcctg gttggaacgc ctgatctgag tcgtgaaaac ttcggggttg tattggagat
     4741 cgattctgtt gatgataccg gcggccatat taccaatcag gacctgtggg tttctcctga
     4801 aggggaagcg tgggtgctgt attcccggcg ggaagtgcag aacgaactca tgcgagacaa
     4861 attctttccg gacagatcgg tgatagattc actgtggctg gtacgggttc gcaatggtga
     4921 aattacacat aaggagctgc ttgttgaagg cacggaagaa agccgtccga cacaggcacg
     4981 ctttcaggag acgcccgacg gcgctctcca tgtgatttta tatctgaccg gtgctgacgc
     5041 gggtaataaa gcgcttcgtg tttttccgga aacaaaggaa agagtcgtgc tgccgctgcc
     5101 cctgcaggag ccgcttcctt ctttctgtct ggcaaatacc cgttccggca atgcaccgtc
     5161 ttatatggtt gacttttatg gcacgcccgg caataaccgg tttcggtatg ccggagtgcg
     5221 ccttccccaa tctgaaaaat gagagcagcg catccatcct tttcctgaaa tgctgcattg
     5281 aaagtctggt atgaaaaaag atcccccacg ttcctatcat ccgacatctg aaaaaccggt
     5341 cgggaaaaaa cctaaacggg tttatgataa agtggactgg aaaccgggca cactcttgaa
     5401 tccggtgccg gttgttctgg tcgcttccat tgacgggaag gggaaaccta atctctgcac
     5461 tgtggcatgg gccggtactg tgtgcagcag gccgcccatg atatctattt cccttcgccc
     5521 tgaacggtat tctcacgacc tgatcgcggc ttccggcgag ttcactgtaa atataccctc
     5581 ggtggaacag atgcatgcta ccgattattg cggtgtcgta tccggcagag acgtggataa
     5641 atttgcggaa acgggactga cacctgcgcc gtcaacaacg ataaagacgc ctatcgtccg
     5701 ggaatgtcct atcagcctgg aatgcaggac aactaaaaag atgctgctgg gtacccatac
     5761 gctgtttctt gcggaaatca aagcggtaca ggtgacggag gcgctgctga ccccgtccgg
     5821 acggctcgct ctggaaaaag cgaggctcgc cgcctatgcc cacggaaact attatgcgct
     5881 cgggaaaaaa ctgggtttct tcggatattc cgtacagaaa aagaaaaaac ggaaaactgc
     5941 cacaggcaag taattttgac caatccacgc caaccgtgat agcatacttt ttcatgtgtg
     6001 tttttcttct ccgcaacatt taaggaaaca gttatgagta tgtccagttt gacgcgcagg
     6061 actttccttg cgacagcaac gacgaccatg gcttttggcg ccgttacgat gaaagacggg
     6121 aaagaagtca ttgaggcgcc gcccgtgaca ccgaataccg ccaaagtgat tccccggaag
     6181 ctttctccca atgaaaaagt gaatcttgcc gctatcggtg ccgggggcaa aggctattcg
     6241 gatcttatga gctgtaaacg caacggcgcg aatgtggttg ccattgccga tgtggactgg
     6301 gatcgctgcg cagaggccat ttatcggctg cctgacgcca agaaattcaa agatttccgg
     6361 atcatgctcg aaaccatgcc ggaaattgat gcggttacca tttccgcacc cgaccatatt
     6421 catgcgcctg ccgcctatat ggcgatgatg atgggcaagc actgctatgt gcagaagccg
     6481 ctgactcata ccgtggcgga ggcccggttg ctgaaaaaca cggcggaaga atgcggtgtc
     6541 atgacccaga tgggcaacca ggggcattgc ggcaacggtg tccgcaccct ctgcgaaatg
     6601 atctcgagcg gcgccatcgg cgatgtgcgg gaagtgcatg tatggacaca ccggcctgtg
     6661 tgggacaatc agggcatgga cgttcctctt cctccggaag tggcacctga aaatctggac
     6721 tgggaccgct ggatcggtcc ggcgccctgg cggccctatc accacaaact tgctccccac
     6781 gactggcgcg cctggcagga tttcggcggc gggtccctcg gcgacatggc gtgccacatc
     6841 atggatcctg cctatatggc actgaatctc gttgaagcga gcgactacac ggtggaagtg
     6901 gtcatgcaga aaggcagaaa cgaacagact ttccccatca tgtccaccat caagtattcc
     6961 ttcccggccc ggggcgacat gccgccggtg gatgtgtact ggtacgacgg tcacgagccc
     7021 gatccgacga caggcgagga agtgtataac cgcccgcagc gtccggcaag cgtacctctg
     7081 gatgaagtgc ttggtgacaa ggaattcaac ggttccttct tcgtcggtga taaaggcatt
     7141 ctgacagccg gcgaatacgg cgataatccc cgcctgatgc cggcagccgc catggaagac
     7201 tacaaattcc ccgatgagac gctggagcgt attccgaggg aagatcctta ctttgactgg
     7261 cttcgcggca ttacagaagg caaaatgccc tgctccaact tcagctatgc aggtcctttc
     7321 actgaaatgg tgaacttcgg caatctggtg gtgaaatccg gccagaaact gcactgggac
     7381 aacaagaaag gcgaagtcat gggcgtgccg aatccgggcg aaatcgtgag taaagaatac
     7441 cgcaaaggct gggaacttcc ctgctgagtg cccctgatcc cgtagaatga tgaaaggcgc
     7501 caccttccgg attcggaggg tggcgccgtt gttttatcag gcgattggca gggtcagtct
     7561 tttttcagtt ccgggatgag ggcgtatgcc ttttccttga tggcttccga atccggagtt
     7621 tcgcccagcc ggcggacaat tgccatggct gcctgaactg cttcagcttt gacctggtca
     7681 tcactaagaa gatccagcac cagaagaagt gcgtcaggac tgtccagatt gcccataccg
     7741 cttaagagca gtttcttttc atcggtgttt tgcacgagag tcagaagctg ccggtaatgg
     7801 gtcagtgctt cctctgatcc gagctgcttt gcggcgagga gccgtgcggc accgcggaaa
     7861 gcggcggtac gctgtgctgt attattgctt acccagtcaa gaagggtggg cagggcaatc
     7921 agggtcggcc agtcggtaat ggctttgaca gcggcatcac gaagtgcctc atcgtcggcg
     7981 cctgtttttt caaaaaggtt gtcaaaggca cgttggctgc ctgtggctgt caggatagtg
     8041 atgagggcag gctgctgcgg ggctgctgca cggttcagcg tgttgatcag atccttcaca
     8101 atgccatccg gaataccttt gtcacggctg cgtgtaataa tggagcgcaa tgcatcttcc
     8161 gctgcagttc tttcctcttt tgactggggg cgtgtcagat gacgcatgaa ttcagaaaga
     8221 tgaccgatgc cgccgtgccg tgcagcgcct tccagtgctg ccaggcggac gggcatagcg
     8281 ctgtctcttc ttgcaatgtt cagcaggcgg ttactcgcat ccggcagccc gcccagagca
     8341 atcattccca ctgccgccag tcgcgtgtcg ctgtcttcgc ttttcagcag acgctccagg
     8401 atctgatcgc ctgcctctcc ggggaagaag gcgaggcagt cgcgggcagc aggagccagc
     8461 tcctcatccg ctgacacggc gagggacgtg atcagcggca gcgctgcttc gtatttgagt
     8521 cgtgtgaggg cattcagggc ggcaatacgc agatcatttc ttcctgccat agcttcaggg
     8581 ataagggcgt gccctgcttt ggtcacgccc agttcaccca gcgtctgaat cacaagcagc
     8641 tttctttcgg ggctgtattc gggcaggagc gcagcgagtt ttgcggctgt ccgggcgcgg
     8701 ggcgtgattt ccagcgcaat ccggagtgcc atctcaaaaa gggcggactc atcgctttgg
     8761 aaggctttaa cgagcagttt ccagcctgca tcggtttcat gagcccgtac ggcaccgcgc
     8821 aaggcggctg cccggacatg taccggaaat gattttttga aatagacgta ttcgtagaag
     8881 ccggacgcat gatcttctct gtcgcgggca aggaacgctt ctgccgtatc catgaaagct
     8941 tctccggtgg cttcccggag agcaaccgga agatcattgc gcagactgaa agtctccaga
     9001 acaccggccg cagctgctgt gcccagcctg ccgagcgcgc gtaccgccgc ttcagcaacg
     9061 gcattatcag ggtcatccac tgccgcagca agagcatcaa aagcgctttc tgtcttgcgc
     9121 acacccagag aagcgatgat tccggcttta acggtaccgg acgctttttc aagtgcccgg
     9181 agcagtgcct gatctgccgc ttcacccgtc atgggttcca gagcataccg tgccatatgt
     9241 gacagcgcac tgtcgttaag cagcggctcc agcacgggaa tggcactttc atcgcctgat
     9301 gcggcaatca ggcggcatgc ctcaattttg gcttccatgg atgcattgga ctgcaggacg
     9361 gaaacaggat cggtatcggc agcgcttccc cggacggcaa gaagcatcag caacagtgtg
     9421 aaaaatatat tacgcatttt atgtactgtc cttctttcag ttgacgggcc ttggtcgggc
     9481 cggttgcggt caaaagggta ttgcttcatt aaagggtcca gggtgcccgt tccgcccgtg
     9541 cgaggaaacg gtttgcttca tcgtcgttaa cgaaacattc ctttttcgga tcatagacca
     9601 gatcccgttc aagccacatg cagatattgg cggcgtggac agtgcacatg gagtgatgca
     9661 tgacccgggg attggcaacc ggttcctgtc tggttttaac acaatcgagg aaattgcgca
     9721 tgtgatccag agacctgcct gtgcgcgctt tgtattcgcc gacgacctca tcgaattcgg
     9781 caagcatttc cggatcggaa acatcgggga cgctgtaacc gtctgcagcg gcaacccaac
     9841 cacgctcgcc ttcgaagagt tcgccacagg agccgtacca gtatttgtcg cctcgtgaaa
     9901 aaatcaattt ctgaccggtg gaaaaatggc agaccatacc gtcaccgctg tcgttgtcca
     9961 catattcata tttaacaggg gatgtttcgc cgagcccgag gcccatctgt gcctgtgcga
    10021 aggtatgagc gccccactct ccgatacagc tggtgtggaa gtcgtaatcg ccccgccagc
    10081 cgcccagcac gtattcttca ttgtaggggc gccaggggca gggcccgagc caggcatccc
    10141 agtccacttc atctttcggc ggttccggct gggcaggcag ccacttgcgc ggcattttcg
    10201 cagcatccca gggtgcgata tgggcatagg cagtggctac attgccgagc cgtcctgtcc
    10261 gcgccatttc aatgcaaaaa acatggttgg cttcgctgag ccgctgggtg cccgtctggt
    10321 agactctgcc atatttagcg gctgtttcca ccacagcccg cccttcggca atggtcatgg
    10381 cggagggttt ttcggtgtat acatctttac cggcgcgcat cgcccggatg gacgcctgcg
    10441 catgccagtg atcaccggta gcaatcagca cggcatcaat atcgggtcgt tccgcaaaga
    10501 attcgcgcat atccgaatac atggcgcagt cttcattgcc gtaatgcccg tcgacaaatt
    10561 ctttcaccgc cagccgctgt tttttctgaa tgtcacaaat cgcaagaatc tgcacatccg
    10621 ggttgcggat catccagcgg agatcatgaa aaccacggcc gcgaataccg ataccggcgg
    10681 ctgtaatccg ttcagaaggg gcaacagccc cgcccctgcc cagtgcagaa ggggggatga
    10741 tcatgggtgc cgccagtgca ccggcagcac aggcaagaaa tcggcgtcga ctcagttttt
    10801 tgctcataaa tattttcctt tatgcatgtt ggcgtgagtc cccgttcggg attcaggcgc
    10861 acatcaaaaa acaactctac cacagccttc tcttttgcat caaagagcag gcagccagtt
    10921 ttttacggtt ctgaaaagct ttcccggaaa tacgcaacgc cttttgaaga aggctgcacg
    10981 agtgtacagc caagagaagg atactgctgc cgaaaaaacg caatggcttg ttctgctttc
    11041 tcgcaaagat caaaaagtcc gaagagtgta gggccgctgc cgctcatggc agctgcatcg
    11101 cagccctgct tcacaagctg ccctttcagt tcagcgagct gcggatattc ggagaaggca
    11161 ggcacttcca ttgcgttaaa aacaaggtgc tgccacctgc tttctttcag cgcccgcagt
    11221 gcccggcgga acgaggagga aaagccggca accggtctgg cattgcttcg gcgcagtaaa
    11281 gggtgcgtat agatgaaggg ggtgctcacc tgtatatccg ggtggatcag cacaaaaaag
    11341 acaggcggga gaggcggcat gggataaagc acttctccct tcagtgtcgc tgcctgggtg
    11401 ccgccgctca ggcagaagga cacgtccgcc cccagtttcc gggcacatgc caggagttgt
    11461 gcaggcggaa gctccagctg ccacaaccga ttcagtgcag cgagtgctgc tgccgcattg
    11521 ccgctgcccc cggcaagccc tgccgccagc ggtatatttt tttgaaggtg gatttgtgcg
    11581 ccttttgtgc agccggtgct ttcttttaaa agcgccgccg cccggcagat gagattggaa
    11641 gaacctgtgt ccagctctgg tcgggaacag gtgagtacga tgtcttcggg gagttcactg
    11701 aaagtcagtt catcatatag gctgacggac tgaaagacgg tttcaatatt gtgatatccg
    11761 tcccgccgcc tgcgcagcac atccagataa agatttattt tggcatagga acgccagtga
    11821 tgcgacatgt ctgaagccag ttatgttttt tcggaagcaa atcagagccc tttgttcccg
    11881 ggcggatgat ttgccaagga tagcacagga gcggccttct gtgaaaaaac agatcaaaga
    11941 tcagtcaaac caggggctca gatcgaaaag aacataggga agtttccagg cggggacagg
    12001 cggattgtta cccgcattgg cgacataaat ggacttgttt ttcggatcga atacacagga
    12061 atgcagtgtg ttgcccgcct gcacgggacc gctgcccaga atcgcggcgg cctgtgccac
    12121 atccatcgca ccgggctggc ggtcaatttc acggctgatc cagtcataac gctgccagct
    12181 gtcctgtgtc ataagcaggg gcttggctgt ttcgatatcc gcttccgaag aaatctcgag
    12241 cccgaattca tgaccgaaag cggcggcaag ccggagcagc tgctccatcc cgactgggtg
    12301 attcgtccgg cgcacagcat ccaccatgcc ccagtgtccc gtggcgtcgc tttctttggg
    12361 atccatcggc gcaaaaacgg tgcagtattt ggcatccgtt tccagagcac gggcatcggg
    12421 aattttgctg tcgccgataa tgaaattcca gccggtggtg cggtggcatt tctgaaaaaa
    12481 ctgaaccgct tcttccagtg tggcgcactg ctccagacaa aggcgcatct gcaggaagag
    12541 aggcagcccg tcaaaggtca catccgagga gggtaacgtc atttctccca gcgtaatccc
    12601 ccgggaattc atgccggtga cgcttccgac accgcctgcc cagcctacca tcatgaaagg
    12661 gatcccccct ttggggcgcc agaccaggat cgtgtgatcg tcctgtgcat cgcctttgat
    12721 gttccagtcc agattcctgc catgaagaag ccgcccgtct gttgtccact tgccccagca
    12781 tgcaaagttg gaacaggcag ggccaccgtc cggcgggaaa tgaagaattt ccgcctgtgt
    12841 aatccctagc cggagatctt cataggaaat accatcgtcc cctgccgcct gcaggccttt
    12901 caccatgcca cgcagttctt cacgaacgga ttcaggaaaa aacttttcca tacgttcaga
    12961 ttgggctgct acatactctt tggagtagcc ccgatcccac aatgctttat tcgtataccc
    13021 ctctttcatg atgtggcgga tttcttttgc cagcagcagg ccgtgctgaa atcccatttc
    13081 ttccggcgtg ccctccatga ccagcatttt ctgatttgcc acctgataaa gtttgccgcc
    13141 tgcctcttcc cggagaaggg tcacttcagg agatgcgttg atttccgctg cataaagggg
    13201 ggcgaaaata cttgccagaa aaaacgaaaa aaagagcgca gaagaaacaa aacgacggtg
    13261 catgcccata gcagaactcc ttatcagtca ccggttacta ccaaataaag caaatgtgaa
    13321 cagaacagca gaggcgccac tgtttgcctt ccgggaaatt agactgacaa caccggcgtt
    13381 cagttacagt tttcgtgtaa aggaggttcg ggttttgtgc cttcattgtt ttttatacgc
    13441 tttctttgtt ttgtttcatt tctcattgaa acaggtggtt taaaagagcc tgcacctggt
    13501 ctgcactgac atcccgggga ttggctttgg tcgatccgga agccagtgtt tcctcgatga
    13561 ttgcctgtgt atccgacaat acctgcccgg taaagggcga ggcaagaggc agtttctgaa
    13621 tcagtgcagc gatcgcttcc agcggatctc ttctgaaaat ccttttcagc gtgtcatatt
    13681 tttcaggaat aacgggtcgg ttgaaagcca gaacaggagg caggcagaaa gcacaaacaa
    13741 gcccgtgagc ggcatgatag cgggcgccca gcggatgagc gagtccatgt actagcccca
    13801 gccgtgccga tgccagagca agtcccgcca tataactgcc cagcatcata tcatcaaaaa
    13861 aagacgggtc accggaacag gcgcctggca gagcgttgaa cactttttcg gcggcgtctt
    13921 ttgccagcgc atcggtgaga caggtggcgc cccggctgca gaaggcttcg attgcctgag
    13981 tgagcgcatc catgcctgaa gcggcaagga cagatgccgg agtaccagtc atcagctcag
    14041 ggtccagtaa aacaagacgt gccatgaaag aaggatggcg gatggatttt ttaacccctg
    14101 tcgccctatt ggtcagcacg gaaacggtag tggcttcaga gcctgttccc gctgtggtgg
    14161 gcacagcgat gaaagggaga ggtgattcag ggatctctgc accgtcgtgg tattcctgca
    14221 cgctgagtgg tgcctgtgac agccctgccg ccgcccgcgc aagatccagg acactgccgc
    14281 cgcctatggc cgccacccag tgcggagctg tgttttgaat ataccggcgc agtgcttctg
    14341 tctgcaccag agcaggttct ccgccttcat gctgccagca gtcggtctct cctggttttt
    14401 ccgacagaag cgcctccagc cttccgttct ttttaacgga tgcactgtgt acgacaagcc
    14461 cctttgtgcc gaaagcggcg cagagcggca ggagtagttt tgatgcgcct ttgccgaagc
    14521 gggtatcacg gggcatgggc aggggaagcg gggcgatttt catgccttat cctcttcagg
    14581 gtagcaggcg ataaaggcaa tgaattccaa agtttcgttg ccgtcgttcc ggatggcatg
    14641 tgattcgcct ctaccggtga ggatggcgtc gcctgcacat acacggcttt gtgtttcccc
    14701 gtcatcaaga atgccggtac cccgcgtaat gtagtagact tcatcttctt tcaggtgctg
    14761 gtgtgagcct atgcctgcac ctggcggaat gctcagggaa gcacaaagcc gcacgggcgc
    14821 cgtaaattca tcggcgtgaa aaaagtggcg caggcttaca gaacctgcgc caccgcgcat
    14881 attgtctctg atttcacagt gcatatcgtc ttttctgcgg atcatgtctt tctccttttt
    14941 tcggttaacc gttcaggaga tagcatgtca aaaaacgctg cacttcggca actttggctt
    15001 aaagggcctt gcttcccagc tgctcttcca gcgaccggca tgctgcctcg aaggtgcagg
    15061 cctccagatg ggtggcgccc acacggacat tggggctttt gccgcagttt tccatgcaga
    15121 aggtggctgt gatgctgacc tgatcggtga gcccctgctg gtccacatga tgcatcagtt
    15181 tcttcagcag atcctgcgaa ccgttaagga agcagttggt tcccacacat acctgtatgg
    15241 gcagcgttcc tttgctctcg ccttcctgca gggcaatgtc aatttcatga agccgcttgc
    15301 gatgtgtgta ttcggtatgc agcgtttcat gggcggtgtg tccgccgacg ccgtcgaaat
    15361 gatcagcata gcatttgatc agttcggggt tatcctgcga tttctggtaa tccagcattt
    15421 tgtcattatc ataaatggcg cgggtgcgca tctgccgtgc ggcaggattg aaggtgtggg
    15481 gctgccccgc accggcgaca cagccgcccg ggcacgccat tacctcaacg agatgataag
    15541 cgcaggtgcc atttttgatg ctttcaatta cggccgctgc ctgtgcgagt ccgttcacaa
    15601 tagcgaggcg cagttcgatg ccgtccactg taacggtggc ttcccgcagc cctttctcgc
    15661 cgcgcacttc ccggaaatcg acgtcttcaa gcggcttgcc ctgtacctgc tcgacggcat
    15721 agcggagcac cgcttcagta acaccgccgg tcacgccgaa gagtacacct gcaccggttt
    15781 tgaaactgat atgcttgtcg aaggcggcag gttccagatc agccagctgg agaccgcact
    15841 ggcgcagcat gagccccaat tcagcggttg tgatgacatg gttcacttca ggtacgccgt
    15901 catgaatgaa tttttcctgt ttggcttcat atttcttggc agtgcagggc atgaccgata
    15961 cgactaccag ttctttcccc ttcatgtcca tgactttggg caggactttg cgtgccactg
    16021 ctccgaacat ctgctggggc gatttgcagc tcgacaggtg aggcagcagt tcgtggtgat
    16081 actgttccgc atatttgacc caggcagggc agcatgaagt gaagagcggc aggacgccct
    16141 ggtttttgaa gcggtccaga aattcttcgc cttcttccag tacggtcatg tcagcagcaa
    16201 aggaggtgtc atatactttg tcgaagccga tggctttcaa tgcagctact gcctgtccgg
    16261 tggtgatact gcccggttcc atgccgaaat attcacccag acccacccgc actgcaggtg
    16321 ccatctgggc aacaacgatt ttattggggt tgtccagatc tgcccagact tcttccattt
    16381 cggggcgcgg tgtaagggcc cctgtggggc ataccgatgc gcagagtccg cagttgacgc
    16441 attccacttc ggcgagatcc tggttgaaag cgggtaatac agcactgtcc gcaccacgga
    16501 aagcgaagtc tatggcaccc acgccctgca gctcgtggca tgcccggacg cagtcgccgc
    16561 agagcacgca tttattgggg tcacgcacca gcgcgagtga cgaatgatcc agcggcgctt
    16621 tgcgttcgat gggtttatac cgcactttgt cgatgcccat gcgttccgcc agttcacgaa
    16681 gtctgcaggt tgcgctgcgc gagcaggtcg ggcaggtcat gtcatgattg gcaaggagca
    16741 gctcaatcga tattttccgg atatcacgca cttttttggt gttggttcgc aggcgcatgc
    16801 ccggttccgg tgccaccgag caggaagcct gaattcctct gccttcaatt tcgaccagac
    16861 agagccggca ggcaccgtac acgctgagct ctgaatggta gcaaaaggtg ggaatatcaa
    16921 tgcccgcttt tcgtgccatt tccagcacgt tgcgctctcc attgatgggc agctcccgtc
    16981 cgtcaacaaa taaagtctgt tttactgtgt cagttgtgtt cgtattcatt gggcggctcc
    17041 ttgtacgatg gcgtcaaagc ggcagctttc cagacagacg ccgcatttta tacatttttc
    17101 ctgatcgatg tgatgaactt cttttctttc acccgaaatg gcttcaacag ggcatttacg
    17161 tttacacagc ccgcagcctg tgcacagctc gtcgataatc acgggagtgc agagcgcggg
    17221 acagacgcct gcggggcagt gcttatcgat aacatgcgcc tcatattctt tccggaaata
    17281 gcgcagcgtg gaaagaacgg gattaggggc ggtttttccg agcccgcaca gcgaaccgat
    17341 cttcactgct tcggcggtct gctcgaggat ctccagagtc tccagagtgc cttctccggc
    17401 gataatgtca tccagaagcg ccagcatctg ccgggttcct tcgcggcaga gcacacattt
    17461 gccgcaggat tcattgcggg cgaactgcat gaagaagcgg gcgacactga tcatgcaggt
    17521 ctgatcattc atgacgacga gcccgcccga tccgaccatg gcacccacgc tgcccagcga
    17581 atcgaaatcg aggggcagat ccagatgttc cgctaccaga cagccgcctg agggaccgcc
    17641 tatctgtaca gctttgaagg gtctgtccgt gacattgccg gcattatcag tcacaccgcc
    17701 tccgatttca tatacaattt cacggagcgt tgcgccgaag ggcacttcaa taagaccggt
    17761 atttgccacg tgcccggtaa gcgcgaaggt tttggtgccc ggcgactttt ccgtgccctg
    17821 tgcccggaac tgttccggtc cgtagcgcag gatcagaggg acgctggcga gggtttccac
    17881 attgttgatc agcgtgggtt ttccccagag accgctttgt gcagggaagg gcggcttggg
    17941 catgggcatg ccccgtttgc cttcgatgga tgcaatgagc gctgtttctt cgccgcagac
    18001 aaaagcaccg gcgccttcca tgatgtgcag ctcaaagctg aagccgctgt taaaaatatt
    18061 ttcgcccagc gctccgaatt cacgcgcctg atccatggct tcctgcagtc gtctgattgc
    18121 cagcggatat tccatgcgta catagatgta tccctcctgg gcgccgattg ctttggcagc
    18181 gattaccatg ccttcaatga ccgcatgggg attgccttcc atcacgcttc gatccatgaa
    18241 tgcgccgggg tcgccttcgt cgccgttgca gataacatat ttctgatcgc ccggctggat
    18301 gcgtgtgaga tcccacttgc gcccggtggg aaatccgcct ccgcctctgc cgcgcaggcc
    18361 cgactggaga atctcattgc agatgtccgc atcgcttata tcgcaaaggg cgcgccgtgc
    18421 cgcttcatag ccgccgtggt tgatgtactc gtacaggtcg cccgggtcga gcatgccgca
    18481 ttctttcagg acacgccgtt cctgccgctg gtagaagggg attgtatctt tgcctttgca
    18541 ctgctcgcct gtctgcggat gcctgtagag gagccgttcc actaccgatt tcttcaccag
    18601 tgaaacatcg acaatttccg gtacgtcttc gggcgttacc cgtgtgtaca gaatgccttc
    18661 aggctccagt gtcaccagag gccccatctg acagaagccc tggcatccgc tctttgacag
    18721 atacacgctg atgggtttgt ctgaatccag cgcgccgtcg gcatggagat ttgtcgccac
    18781 ttccagaccg agcgccgtga gttcccgcac aaaggcgtcg cgtacggcca atgcgcctcc
    18841 ggcaacacag ccggtgcctg cacagatgac aattctgtgc tctacccgtg aaatacgctg
    18901 ctgaaaaata tcggatgcct ctttgaggcc gggacggtct gtcattactg atgtattcat
    18961 tgctgtgcct ctctttctat aattttgtcc agcgcttcca cacatttctg gggcgtcatc
    19021 atcccgtgca catcttcatt gatgaccagc acaggtgcca gtccgcaggc gccgagacac
    19081 gataccgtct ccaatgtgaa gagcagatct tctgtggtgt ttttatcagt ggtaagcccc
    19141 agttttttct gaatggcatt cacaagaccc tgggagtttt taacgtgaca ggcagtaccg
    19201 tcgcatacat gaatcacata ttttcctttg ggttccagtg cgaagtgcgc atagaaggtg
    19261 gcaacaccga aaacccgcgc gggcggcaga ttcagagagg tggcaatata ggcgagcgcc
    19321 tctttgggca ggtagcggta ttccgcctga accgcctgca aaatggaaat cagttttgcg
    19381 gggtcccggt cgaaacgatc aataatgtca cagagcgtat cgaattttct catggtttgc
    19441 tgcatgaccg gtttctcctg ttgcctgtac attaaatgca cagcgtgtgt gtttctttta
    19501 aatagcgttc ttcaagacaa tgggtaagcc gcttcacaat attgcggcgg atttcaaggt
    19561 caatgggaag ggtcgggtcc tgatgggctt cgttgatccg gctcccgacg ataaagtgga
    19621 tgatgtcgct ttccagaagc agtgcggcga gtttttcagc aggatcatcg ccgcgcactc
    19681 cggcgccttt ttccagaagc tgcgcggttc ttgtgagggt gaggatacct tcggtgatca
    19741 gatccacgcc ttccatggtc gattgcggcg gaatgtgctc actcatccgg ctcaagtcca
    19801 gtttggcctc cacgcccagt tcccgggcaa cgatggttgt ggtggtgccg ccgcaaatga
    19861 cttttcgtcc gtcaaactcc tgaagtcggc gggcgaactc cgcatcatgg ttcgggtcaa
    19921 agggcggccc ggtcagaaca atcattttcc ggggcttgcg cagatagaga acggcgcagc
    19981 tcatgtcgtc atgggcctgg cgattcaatt cccggctcaa tgcttcacgc agcacatctt
    20041 ccgccagtgt gcgcgccgaa accacgggat cctgccggat gcggtccagc acaaattcgc
    20101 gcagcccctg atcgcgccac cccagaggaa gcgccttcga gccgagccct gcctgaacga
    20161 tgccgtccga aaccagaacc acacgatctt ccggcatggt ttccatgtca ctgaagcgca
    20221 gcacacgatc ctgccacttc ggcgaaacca tttccctccg ttccagcggc agtgccttgc
    20281 cgtcgcggat gaaaaggagc ggcggattat ccatttcaat aaaacgggca cgcccgcctg
    20341 ccgaacagtc gataatcgta aaggttgagt agctgatgcg tcggatctgg cataccggca
    20401 gggcgtccat gatcacttct gcagaatgca caaagtccat gctgcttgcc gcaaacttca
    20461 gtgccatgga agcggtcatg gttgccagaa tattggcttt tacaccgctg cccagcccgt
    20521 ccgacagcac ggcgagaatc cggtcgctgc cgtccactcg ctgcgattga aaggcatcgc
    20581 cgcagatgtg ttccgtgtgt ttgcgccgct gtgcaaaatc aacttccaga aaaagagtat
    20641 cctgattcat cgctctttct ccttcggttt gcccgcgaga cggccggaat cacaggcata
    20701 gccttctgca atgctgcgca gcagaatttc tgtatccgcc atgtgttcac ccaggcgaca
    20761 tgcaatctcc tgtactgttg aaatattgcg gcggatcact tcctgcgcct gctgggcaat
    20821 ctgatcgcgg agtccttcct gccgggttac gtcgagaatt acggcaccga ctacctgatg
    20881 gggatccagc gaaaagacgg tgatgttaag cagccggttt ttatagcgga aatgatcgta
    20941 atgaatttcc tgatcaaatt tgagtactgt ccggaacata tctgtgaagt cgatgatttt
    21001 tgcaagtgag cagcctttca gcccggggca ggtctcatag atttctgtcg tttccggacc
    21061 gaacatgcgg gcaaagcgct cgttacattc cacaatatgc atggcggcat caacaatgac
    21121 gacacccgag ggcatgctgc gaagcagggc gttggctttt ttctgtgcaa gtgcacgcat
    21181 atgggagacg cacatggtct cttcggctgt gccgcgtacg agtgccgcag cgaattcccg
    21241 gcaggtgttg tagccgcagc ccgagcagtt caattcgtct tcgggcagtc gtttccctat
    21301 gcgccgtaaa gcaccgcgga caagtgccgg atccggagcg ggcggtgcag caggggctgg
    21361 cgtgaaggtt tcggtgatgt ccagtgtatg ctcgcgcact acaggcagag gcggtattat
    21421 ggcgtgatcc tcgacgagaa ttcttttgcc gaggagtgcg cctgatgcct tggcgcaggg
    21481 gccgccgatg catccgcctt cgcatccaag cgcttccaca aagacaggat gctgcaggct
    21541 ggtctcttcc aggcccttca gcgttttgca gatattgtcg attccgctga ccgctacata
    21601 acgtacagtg tctttggcgc ccagcgcctg aatggtagtg atcatacctc cttccaccgg
    21661 atagagtgcc ccttcgtcgg cgctgaaagg aacaaaacgg tcgtttttat ccggggtcat
    21721 ggtatacgga tccagcttca tctgcctgaa ccagtcgttc agttcttcaa aagtcagcgc
    21781 aatatcaagc aggtcccggt gctcgtccgc ttccgttttt ttagcgatac agggtccgat
    21841 aaagacgatg cggatggcat caccgaaatg ctgccggagc agtttgctgt gtgccatgac
    21901 aggtgagtgg accgcggtga tattggcggc gtaatcgtgg aggtatttgc gcacgaggcg
    21961 gactgtggac ggacacgccg acgaaatata caggccgttc ctgttcagca tctttgctac
    22021 agcagccgat acttcctgcg ctccgagcgc tgtttcgctc acgcccgcaa agccgagccg
    22081 tttcagggcg cagatcatct gttccgcctt caatcccggg tattcacctg cccaggaagg
    22141 tgccagcgac acataaaccc cggcttcctg atcaagaaga tgccgtgccc gccccagatc
    22201 aaggcggatt ttctttgcct gtaccgggca gacctgcacg cagatcccgc aggcaacgca
    22261 cagatccggc gtgattttgg cgtgtccgtc ctcaatgcgg attgctttca acgggcactg
    22321 gcgcaggcac ttgtagcagt cctgacaggt ggtttccatg gtgtcgatgg ggaagaggcg
    22381 gtccatggct tattcctttg catttaccgc aaaatgggtt tcaagaatgt ccagaagcat
    22441 acccgggtcc acatgctgat aaagggtgtc gccgatacgg atgttcggac ccttctggca
    22501 ttccgcatca caatggcttc cttccagacg taccgcagcc gtcaggttgt gcttttccag
    22561 aaagttttct atgagcgtaa gattgttccg gttgccccgg gcaaagcagg aactgcccat
    22621 gcagatgact atagtgacag gatcgttcat cgttcacctt ccttgaaatg gtcgtcttca
    22681 ttcttcagta cggcagccag cttgctggag agaatgccga gggaagaaaa aagatcttcg
    22741 ttctgtacaa tgatgccttc aggcactttc agtgccaggg gtgcaaagtt ccagattgcc
    22801 agaatgccgt tcatcaccat cagctcgccg acggtctggg catattcggc gggcactgtc
    22861 agaatgccca gatgcacctt catccgttcc agcagattgg gcatccgtgt gagcggaaga
    22921 acggggacat catggatctt cctgccgact ttttcagggt ctgtatcgaa ggccatcaca
    22981 atattgactc cgtactgatc aaaggctttg tagcccatca gcgccgagcc cagactgcct
    23041 gcaccgacca ggatggcgtc ccgggcattg ttccatccca gaaagttctc cagctgccga
    23101 tgcagatccc gggtgagata gccgactttc gggcggcctg tcgcaccggc aagagcgatg
    23161 tccttgcgga tcagcgtagg atcaatatgg agtgcttcgc tcaactgggt gcacgataca
    23221 tattcctgcc cctgctcggc gagtcccagc aaaacctgac aataggaagg cacccttctg
    23281 agcgttggtt cggggatgct cctgatggtt tcttctttga tcatgtgttt tctcctttcg
    23341 ggtgcctaat agagaaatag atatcgagaa atatatatcg agaagattat ctctaattat
    23401 acaccgagaa aaaacaaatg tcaaattcag atacccgcaa aaagaagaga ccggaaaaaa
    23461 aggcgcaaaa gataagcgcg ggaagggaaa atacccggag cagagggaaa cgctagaaca
    23521 gactacgcaa taaatctttt tgtttttcgt agtctttccc tgcaatttca caaataggaa
    23581 tttgggagcc aggctaaagt ggccttgaat caggcgatta ccgtctggaa atacggcctc
    23641 aattcattgg cattaggcgt gcttgcaaaa tcgaacaagg agaaatgcga ctagaaagca
    23701 atatgaccaa cttgtagatt gccgatattt tctaaatcaa tcatatattt ttctggccgt
    23761 ggttttccgt tgctattgcg cccttgtttc atagtgctga gtagttctaa atccgcacag
    23821 gtaaatcgta gaaacagaat ttcagtcaag acggctttcg ttttacgata gcgtgaaatg
    23881 cttgttcttg ctttacgctc aatttcatat gttgacagat cacccttaag ttccgtgtgc
    23941 cacttttgaa acgaacggtc aatatcatta tattcaacat cgcacaaggc tatgatcact
    24001 ccgtgctccc caccattttg agcgatagac tgtttcattg cccttacatc atttagaatc
    24061 gccctgtggt catctgattt tatggcttta gctttcaaat cccagtttac attgcgcctg
    24121 aggtcaaacg taacgctgtc gaagtgaccg cctggatgct gaaagtcact aagtaatact
    24181 ttgtaacaca aatactcaaa gtaaaaagcc caccattcca tctgtttcca gttgtagttt
    24241 gcttgcttga gttcgagaac gctaggcttc ccctcccaga ctgtttttag tgaggctaat
    24301 tttgccttta gaatattcag atcttcatta aattgtgtcg tcattgaaag tgccatcctc
    24361 cgtgtttgcc acgcccatgt ttttactttt ttgaaggtca aatagtgaga gttgcacatt
    24421 tttttcgatg ttaagtcgtg taagggcaat atcacaatac tcttcactaa tatcaatccc
    24481 aagatatcgg cgttggtttt tggctgcgat aacacaagtg gtaccgctac cagccattgg
    24541 atccaaaaca aaatcgtttg gcatagaaaa acattgaata atatcttggg ccaacttatc
    24601 tggataggtt gccgggtgtt ttaacttaag tctgttccct tcagtgttac ttgtagaata
    24661 gcgccaaaca gtacctctac acttcattga atttacttct ttcggtacta tttccttgaa
    24721 gcccccattc gtcaatctat ccgtgccaga atacactttt cctgcatgtt tacttggtat
    24781 cataagtgat cttttttcaa aggtcttagg gcgctttccc ttaaaaaaga gaaggatata
    24841 ttcgtgatca acacgaaatc tttgtgtcca ccatgcgcct ggattcccat cacgatagta
    24901 aatgcattgt tcgaataagc gccaccctgc attgtcacac cagtcaaccg tcaagcgggc
    24961 tgttgttaga gatttagcga agtcttttgt tccatcgcca ataactactg cacagacacc
    25021 accttctttt gaaactctat acaaggattt tcctaaagcg gagtaatcaa aattccaacc
    25081 tttgtgataa gtgcgaatgc catcgtaagg ggggctaaaa agaatgagat ccacttcggc
    25141 gtctttaata tagggaagta cttctaagca atctcctgtg atgatgcaat tagtgaggtg
    25201 attattaaca gcgtcattaa ttgataaatc ggccatagtt agtttatttg ggcttgacat
    25261 gatttaaaag tattccttta agtcagattg ttgaccaaaa tatcgacttg ctttagcaat
    25321 tcaatgcatt catcgcttag gaagccgcgg tcccttttcg gggcttgatg ctcattttct
    25381 caggggcgca cacattattt tttttcgttt tctctctgta tttcagatat gtaacctgtt
    25441 cgaaatcgtt gcggctgaac aaatccatgt cctgattctt gtggcaaaag agagcgtata
    25501 tcttttcaca aaaccaccgc cgccacatta cttgaccagc tgacgccact ggcgttgacg
    25561 gctattatac ggtattcgag ttgcataaaa cgaagttggt tagtcagggc gatttcggtt
    25621 tccaaggcga gtcccgcttg cgaccagtcg ggaaattccg ggacagacta cgcaataagt
    25681 tatttttgtt ttgcgtagtc tgtccccgca tttagaggac attgcgtttc gatattgaac
    25741 ttgcgattac cggcaaaaaa aggtgcaaaa ggaaagcggg ggaagggaaa ctatccggag
    25801 cagagggaag agataaaaaa agggcgggac cgcggcaggg gaaggggagg ggaggaagtg
    25861 gggggaaacc tgccacgatc ccgcgttttt ccgaacaagt agtacaacct tgtccagctt
    25921 gctacacctg tatagaatac agggcgagtc aaaaaaactt aggagtcgtc tttacgattt
    25981 aactctctgt ttagtgaaac acagttgtgt gcatttacat tcccggcttc aactcttttt
    26041 ttcatctcta ttctactaac gaatttttga taagaaaaag ttaacagact ctttctgata
    26101 taaggaagaa agagggtgtt aagggtcttc ttttttaaaa tggcaatgac atttcataat
    26161 ttgatatgct aactgacagc cgttcactgt gaccggcaag taattacaag cgaccggcgc
    26221 gagagctatt ttgatctcgg cagccatttc agcaaagaga ggattacacg tatggaaaag
    26281 aaacccagca atctttcccg ccgggatttt gccaaggcat ccgctgcagc gggttttgcc
    26341 atcctggcaa gccgttccgg tcaggcgcag gacagcgccg aaaccctgaa aatcgggctt
    26401 atcggctgcg gtggtcgcgg ctgtggtgcg gcagtgaact gtctgacagg caacgacaat
    26461 gtgaagcttg tggcgctcgc cgatgttttt gaggattcag tgattaaggc aaaagccaat
    26521 cttgagcgga acaatgaccc ccgagtgaaa aacaaacttg ccattgaaga agaactttgt
    26581 ttccacggac ttgatgctta ccagaaactg ctggagacgg atgtggacat tgtgatccat
    26641 gcgacaccgc cctattgccg gcccatgcat ctggaagcca ttgtcgcggc aggcaagcat
    26701 cttttcagtg aaaagccttt tgctgttgac agccccggta tccgccgctg cctggcagcc
    26761 gccaaagtgg ctgatgaaaa aggacttacc tttgtgaccg gtcttcagcg ccgttatcag
    26821 gacaagtacc tccagacgat cgagaaactg caaggcggag aaattggcga gattctttat
    26881 gcccgcgtct actggaatgg aaatcttcct ttctgtcacg accgcctgcc tgatgaaagt
    26941 gatctggcct accgtctgcg caactggtac aactacatat ggacatgcgg tgacaatatc
    27001 gttgagcagc atgtgcacaa tatcgacgtg attaactggc tgcttggtgc gtatccggtg
    27061 aaagtggttt ccaatggcgg ccgtgcctgg aagccggcgg aagaaaaata cggagatatc
    27121 tgggacaact tcgcctgcga tttcgagtat gccaacggtg tccacatgct cagcatgtcc
    27181 cgccactgga ataacagcaa gagcgatgtg tgggaacggg tagttggcac caaaggcaaa
    27241 gccgcttgcg gtgatctggg ccgtgaccgg agcgacccct atgtgaacga acatattgcc
    27301 atgcttcaga gtatccgtgg cgaaattgac cgcattaacg atggtgagcg taccgcttat
    27361 tccaccctta ccgcgatcat gggtcgtgaa gccgcttata cggggcagga agtcacctgg
    27421 gaacaggcgc tcaactgcga aaagagccgt gttcctgccg atctggattt cgcaaaatcc
    27481 tacccggtcg ggcccattcc cgtccccggc gcctgatcct tttaaactga gtccgattgt
    27541 gcctctcctg tcctttccgg atgggagagg ttttttgttg acagaaagtc agctaaaaag
    27601 atggatcaat gtcacggctt catataaggc ggcggccgca agaatcacag cagtaagcag
    27661 cgccgcatta aaaagcagcc agctgccctt ccggatagcg gccgatacct gtcttttacg
    27721 gatgccccgg aggagcagag cggaccagag cagcacaaag aagcaggcaa tgatataggc
    27781 ttccagttcc acgaccatgg tgaggcagtg cagtataagc accgctgctg tgccgaccca
    27841 gaggggggac atggcaccgc cgacaatgaa aaagctggca atatttttca gaacacccag
    27901 gggcagaatc agagaaagag cgaaagtcat gaccagggtc tgttcgatgt agttgttgta
    27961 aaaagttgcc caggtcgcct gcaggatatt gcctttatcg taggcgctcc caatatattc
    28021 cagactgccg tcgctgaaaa tgtccatagt ataacgtttg agttcatagc ccatgagagg
    28081 gttgctgatg gctgaataca tggacagaaa aaaagcaccg taaagcagca gatgcatcag
    28141 cataaagaaa cgacggtgct gctccattgc tccgaataca ggtgcaaaaa ggcggcgctc
    28201 ctgcagatgg aagcgtctga acagtttgat gaagaaaagg ctgccgccca gagcacacag
    28261 gagcatgctc agaaaactgc agcgcgccgc ataatcatgc gtgcggagta ccccgccgat
    28321 aacggcaggc acggcaataa ctgcgtccgt ctctttgccg ggaagttcat ccgcatctgt
    28381 ttcagaagca accgggtgcc ggacttttcc cgaaggcggt tcagggatca taagcccgtt
    28441 ttcaggtatg aataccccct gaaatacttc cggatcagca ttgtcaaaaa gttccgcaaa
    28501 agcgctccgg tccggaagca gataactgca gatgaacccg gaggcggcag gatgcagcct
    28561 ccctgtaacg gtgaaggtta tgccgttaat agtaaaaggc tccggtgaag cgaggtctcc
    28621 cgccagtact tccgcctgcc ccggttcagg aaggcgacct gaaagaagag ggatctgaag
    28681 tgtttcagaa gcaagatcca gagcgaagag catttcccgg cgcggtgcgg tttcgctccc
    28741 tggtacagtg gcgatacctg ccagcatatc aagccccctg tcgtcggtcc agagccgtgc
    28801 gtcaagaggg cgggcggaca gcgggtaagc gatctgttcc cgcggtacat ataccggtgc
    28861 cagatagatg gccgccgtac gccagggtac ttcatggctg tatgccaaat cgataagcgt
    28921 gctgaagaag agccctgcaa tcccgacgag aaaaaggaga atggcaaaga agatgccttt
    28981 gcgggaaggc gctgtttttc ccaaatacca ccgtatttcc gccggaggat agccaggtgc
    29041 gatttcaatg atttcgtggg gcttcatgga gctgccgcca gcgtgggtgc agatttgccg
    29101 agctgcatga ccaccctgtc cagaatctct ttaaaatagg tggtgctgtt ggggcccagc
    29161 cagttcatga acccgatttc atagtccaac tgaccccgtt tgtttgtctc gaaataatat
    29221 ttttccggtg acagataacc taaataggca cgggagaaac tggtaaccca gagatcccag
    29281 cctgctgcag ctttttcttc acgccactgg cgggtaagtt cgccgcttgt atcaaagggc
    29341 agccccacaa aaagcaggtc accgatgcgg gcgccctgta cgaagccttc gttgggaagg
    29401 ccgataagtt ttgccgccag aggtgagagc cgccatttcg gggataaggg ccgtgcctga
    29461 aaagggggca cgcctacagg tacggcaagc gatgcaatgt cgacacgatc tgtgaactta
    29521 ggcgacgcac ttgccgccac gatcagatcg gctagtgttt tgcccatcaa ttccactctt
    29581 gcatccggcc cgtccgcctc gatgcctttt ccggacatgg agccaagccc gccgcccagg
    29641 tatacggcag tcgcattggt ctgctgttcc agagagcgca tcagcgcacc cggatattcg
    29701 gcggaaaact gcatcatgga ggaaccgaaa ttcgtgggat gagccgaaaa acgggtaata
    29761 taacatttac gtccgctttc ctgctccacc agaatatagt tcagattggg atctacatcg
    29821 ccgtgttccc gggcacggtt acggatatat gcccgggcgt ccacagtgcc gtgggcgagt
    29881 tttgcgggct tcatattctg ccaggcttcc acaatggcat cggcgaaact gctcgaaaga
    29941 aactcgggca cagaggggtt ataagtgccg cccgtgaatt tggcggcaag cccggggccg
    30001 aatccgccgg gtccgcagtg agtatggctg gcggtgaagt acaaattccg ggaagcgagc
    30061 ggtatgcgcg atgccacttt atccaccgtc atttctgcaa tattcggggg aatgatcagc
    30121 atgtcaacgc ccaccagaac cacggtgtcc ttgccgtccg tgaaagaaac agctttcacc
    30181 ttcagatcat cacggacacc ctgagagcgc attccgtctt tccgtgcgcc gtagcctgca
    30241 atagggagcc cgatagaagg cgtaatgatg cgtactcccc agccggcacg gagggcttcg
    30301 ggggtgtctg tgaaattcga tagtgccgca tcggcatcaa cggcagcgag ggcgtctttg
    30361 taatacgccg actcccgata gcgtgagtct ttataaaccg gccagggacc gacgaagaca
    30421 agaaaaagag tggtgaaaag aaggaggaca acgacaacac cgagcaggat acgcttcact
    30481 atatgcataa cggaaactcc cggatccggc aaagaaacag tattccggat ctctgttttg
    30541 ctgcctggca tccgcctcaa gccacagcat caatgtgcga catttgaatg cagaagcagg
    30601 cagggaaaag atttacaaca ggctctgttc agaacggcct gctgctgtca gaatttttgt
    30661 gttgtacttt caaaaaaggc tttttgagag ccttttacca ttcgatagat gccttgagca
    30721 cgccgtcacc gtatgccgaa gtcagcgcaa aggcatccgg cgcctcatca aaagagaaca
    30781 catgggtgat caggctgtca acgtccagtt tcccgctctg gatataatcc agcgtggcgg
    30841 ggtaattgtg cacaaagcgg cggcaccact gcaagcacag ttccctgcgc cgggcaatac
    30901 tgaccgggag cgggtccatc tcattgcccg agattcccgt aagaacaacc cgcccgcccc
    30961 gacgtgccgc cgcacaggca agggcaagcc cgtccgcgct gttggtacag tcaaaagcaa
    31021 catccacgcc ccggcgttct gtcatttcct gaatccagcc aacggcggag gcgccgcctt
    31081 ctttaataga accttttctg ttgcagaaga cttcctggat gccgtagaga cgggccgctt
    31141 ccacgcgata aggaagtatg tccgcagcca gcacccgggc agcgccacag cactggagca
    31201 tgcgggctgt aagcagcccg ataacgccca ggccgaaaat agccgccgta tcaccggggc
    31261 gaagctgtgc cagctcagcc gtgtggacgg caacggcagc gggttccacc atggcagctg
    31321 tacctgcaga catggtgccg ggtacgggaa agcagaactc gtgatccacg gcgatatatt
    31381 cgcatacggc accgtctgag cccggtcctc ccggaaaaaa gagggaagtg cagagattgt
    31441 atcggccttt ccggcaccac tcgcagcggc ggcagggaat gccaggctcc acagccaccc
    31501 gttttcccag cagcgccgga tcggcatcgg caccgagttc ttccacaatg cctgcatatt
    31561 catgaccgag cacggtgggt gcggtaaggc ggttttcacc tatcgccccc tccagaaaat
    31621 agtggatatc cgagccgcac acgccgacgg cttccacgcg tatcagggct tcatttcttt
    31681 tcggtacagg acggggaagc tcaacaagcc tcagcttctc cggtgcctgc cattgccagg
    31741 ctttcatagg cgaattcctt gttattgata ttgctttgtg caaaaggaca aaagtgcaca
    31801 gggcacagag ctacggtttt ctttttttac ggccattctt ttctgtttcg tcgctgtcca
    31861 gcatgaaata aatatcggaa accagctctt tttttgccat tctgttgatc cgccggagca
    31921 gatcccactt gacatagctg attttatgca tccatacggt gctgtcggtc ataatgcgca
    31981 gctcgccgtc ccggatgtcg taggggcggc agtgtggcgc cagatgctcg ccaacaagct
    32041 ctgtccagtg ctcccagatt tgcgcctgtt ccagcgtcag cccgagaggg gtcgtttttt
    32101 tcatccggtt caggatgtcg cctatatgtt caagttccga ctttttcaag tgttcctttc
    32161 tcaagcctga aaatccggct gtccgaccgg attgtttccg gaagctgggc gggctgcgcc
    32221 gtagtaatca gtgtctgtgc cccttcagga agggcatgaa aaaggcggtg cgttctgttt
    32281 tcatccagct ctgccaccgc ctcatccagg aggatcagcg ggtagtcgcc gagccgtccg
    32341 tgaaccagtg ccacttccgc cagttttaca atcagggcgg cgctcttctg ctgaccctgc
    32401 gaggcgaaga cccgggcgga gcgcccggaa atcagaatat ccagatcgtc ccgatggggc
    32461 ccgcgtcctg tactgcggcg ttgctgatct gtttttcggg aacgttccag cagggaggcg
    32521 aattcctctt tgtccggaat atccggtgca tagatgattt ccagaggttc cttttccaca
    32581 atttgactgt aaaggggcga agcgatggcg gaaagctgcg caatataggt ggtgcgttca
    32641 tccatgagcc gggagccctg agccgccagc tgcgcttccc agagacccat cagctcggca
    32701 tcgaactgaa gatgtttcag catttcattg cgctggcgaa gtgcctggcg atactgctgc
    32761 aaagcgcgga gataaggggt gctgagctga gaaagctcca tgtcgagaaa aaggcggcgc
    32821 acagcagcgc ctcccttgat aagggcaatg tcatcgggag agaaaaagac gacgcaaagc
    32881 tgccccagca gatcgctgag ccgattctgg gcgacgccgt tcactttgaa gcgtttggca
    32941 cctttccacc agtgcgcttc gatagacaaa gaccgtttcg ctgtttcccc ttcaagataa
    33001 atgtgaaagt cctctttttc gtggcagaca agatcagcgt cattggtggt gcggtggctg
    33061 cgggctgtgg cggcatacag aattgcttcg agcaccgaag ttttgccctg tgcattgcct
    33121 ccgtagagca gattaatgcc cgctccgggg atcaattcca tatcggaaag cccccgaaaa
    33181 tgccggcatg tcattttggt taatttcatt taatctgctc cggaagaata cagccccttg
    33241 acagcctcag tattatacac ggaaataaaa aaagaggaga aggatccata tataccttag
    33301 caaagggcgc ctgttgccgg agtaaaagtc ggacggcgcc agcacagtag tgctgacgcc
    33361 gtcgtcaggt tatatcaggt agaggcctaa aaatcagtca cgtttttttc gaagtgcggc
    33421 tgctcctgct gccagagccg cagcggaagc caggagtacg cccagcctgc tgagggcggg
    33481 aacgcctgtg cccacgctca acgttaccgc agcggaattt actgtaatca tatcgtcgga
    33541 gatagtgcag cggtagtctc ccgcatcgtc aaagtcgaca gagctgatga caaaggtgga
    33601 ggcatcgtcc gcgccgggaa tgggctggaa atcgcccgct ttgccattgt cccgtttttc
    33661 ccactggtaa tgaagagcac cgacatggca tgaactgctc tgctccagcg cagtcatggt
    33721 tactttttct ccgggcactg ccgggttggg tgtaatatcc agtgtgacgc ggattttgca
    33781 gctgtcccgc ttcacttcta ccgtccgtac ggctacggtt tcattgccgt ccgcatcggc
    33841 tactgtgtag gttacggcat aactgccgat tacaagagga ttcacatcgt ccccgccttc
    33901 gatgacgatg tcatcggtgt acacggcatc accgcagaaa tcaatggcaa cggcgccata
    33961 ttcaacatac gctgttccgc catcgaggac gaggggatta tcaccggtga ggatgatctc
    34021 gggtccttcg ttatcggcaa ttcgcacagt ccggctcacg gaggcagccg ggttcccggc
    34081 atcgtctgtc acgtcgtagg taatcgtaaa gacagcaccg gagacggacg tatcaactgt
    34141 atcacctgct acaaccacct ttgcggtcag gtcgccgtca catgcgtccg cagcggtcac
    34201 taatggatcc acaaagtcgt agccgcaggg cgcttccata tccgcatcgc caagaagcgt
    34261 aaggatcggt gccgtgctgt cctcgactgt gatgtcgcgt gttaccgtgg atatattgcc
    34321 cacagcatcc tcagcggtgt aggtgacggt atagattccg gtggctcccg tgttgataac
    34381 accggtaatg acaacagcga cattgccgtc gacgttgtcg ttggccgttg cgccgggctc
    34441 agcgtattcg tctacgccgc attccagtgt aatgtctccg ccattcagtg caatcaccgg
    34501 cgcaatcccg atagcgccac cagcatctgt actctgattt ccgtctatag gattgccgaa
    34561 attcccggta aaggcgggat caacggtgat gacaatgtct ttgccgttca gcatttcgcc
    34621 tgcattatcc caccagaggc gtaccgcttt ggcgcctgca atttccacgc gggtggggtt
    34681 ggtgctgaga gatcccgcac cgttccccga gaccgtgtaa ttggcagcca caagtgcagc
    34741 attcagatag gcaatgtcct gattccaggt gaccacgacg gagaattcgg aatccacttc
    34801 caccgatacc agagacggat caatcagact gaccacctga acattgcgga ctacttctac
    34861 tgcgtggttg ccgtttgcat ccgtggcgtt gtaccggatt gtataacttc cggcgacatt
    34921 gggatttact gaacctgtgg cgactacgct caccgtggtg tcgcagctgt ccgcaactgt
    34981 tgcaccttca tcggtgtagg ggtcacctgt gaagatctgg atgttctcgt ttccgatgcg
    35041 tgtaatcacc gggggggtcg tgtcttccac cgtaacggta cgggttgccg ttcctatatt
    35101 gccttcactg tcttcggctg tataggtaac tgtatatacg cccggagcgt ccgtgtttac
    35161 agaaccgctg ggtgtgacag agagcgctcc ttcgcagctg tctgttgctg aggcaccggg
    35221 ttccgcatag ctgtccacac cgcattccag tgtcatcgct gcggcaccgt taagcgtaat
    35281 cgccggaggg gttgtgtctt taatggtgac tgtccgggtc tgctgcagtg cagccagccc
    35341 cgctttatca ctaacgttat aggtgatggt atgcacgcca acagcggtga gcgggaaggt
    35401 gactccgcct acagcgatca gcgtggaatt aatgggaccg tcttccgcat cgtttgcagt
    35461 cacgccgacg cgggcattgg ctgctgtata agaagcaccg cattccagag tgagcgcccc
    35521 cgggatggcg ctaatggttg gccgggtgtt tgtgaaggct accgtgacgg ttgtatcggc
    35581 agtcacattt tcaagcgtga aattgggtgc tgtaccggaa atagtgccat tgcttactgt
    35641 cggtcctgcc agcacgtagc ctggattcac atctaccgtg aagttgacag tgccgatggt
    35701 atcctgaata gttccggaca gaccggtcac cgtgccgcct atcgcagcgc cgtcggggct
    35761 gttggtggag acagcgggag tgatgttgtg atacaccgcc gtaaagtttg ccgtcacggt
    35821 cgtatcagcg gtcacattag agagtgtgaa attcgggtaa gatccgttca cagtgccgga
    35881 ggaagccgaa atgcccgtga aagtatagcc gggatcggtg gagacggtaa ccggaatgga
    35941 tccgaccgta ttctgaattg tatcggcacc ggcgacagag cctcccgtat cggccgagta
    36001 gccgacggtg tgtatgatcg gctgtacatt ggcattcacc gttgcattgg ttttaacatt
    36061 cgtaagtttc cagttggcac cgtcagcagc aacggttcca ctcgtcgatg tcggggcgcc
    36121 tacaagctgg tagccgggct gaacagtaac actgaagatg gccgttccaa tggtgtcctg
    36181 gactgtagca ggcccgctga tcgtcgcgcc agtgggggtg ccataagtca agtttctcat
    36241 gacagcgtta aaagacgctg tcacggttgt gttgacagtc acattgctca gcgtataatt
    36301 cggaaaggtt ccggtcagag tgccccgggt cgtggtgata ttggcaaagc tgtagcccgg
    36361 attggcggcc actgtaaaag aggtggatcc cgtacggggg aatatggctg cagcaccacc
    36421 cgtgatggtg cccgtattag cgggggttac agcatatgta attgtgctct tgacagggtt
    36481 aattacagca actgccccaa cgccattccc gctgtaactg ttccagttgg gcttcacagg
    36541 acccacaata tttccggtaa taacctctcc gagtccgggc cggtagtcat aacgaactgc
    36601 gccggcgtta taccagttcg gccattttgc gtattgctgg ttcacagttc cgccgcctga
    36661 aacatagtcg tttctgttaa tggcatcgaa cttggtgttg taaccgctta gcccgacaac
    36721 aatgttacaa cccagaggaa gatctgatgg agccagagga actgcttgaa aagggtcacc
    36781 tgaccattca cacgtattct tgcctatagg ttcagaggga tcattgcctt tgaaataaaa
    36841 aatgcccgcc actttccctt taaagtttcc gtccacatat gaagttgcca aagataccgt
    36901 gtttttgggc gggttgacca gataaaacat gcgaatccgt tcataattgc tcccgaaatc
    36961 cgttgcagga agggcaatca tgggggagcc gcccaaagtc atggaggcgg ttttactgtc
    37021 tccattgtgg gcaattccga cagccagcgt cgtatcagcg ggaaccgtca cattaaaaga
    37081 gaaagagctg ttgctgctgt tattttctcc tgattcgctg ccgatgaata ccacctgacc
    37141 atgcgcgctt gtgatgagtg ttgccatgat aagcacggca ggcagcacac tgatccactg
    37201 cgccacacct cccctggctt tttttgttag gtaacgtacc atagtttctg taaattaatt
    37261 ggacaaacgg ggtcatcgtt tgg
//
LOCUS       DAOPWB010000012        36348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1106_length_36348_cov_5.474417, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000012 DAOPWB010000000
VERSION     DAOPWB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 36348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36348
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1106_length_36348_cov_5.474417"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKN92_02320"
     CDS             <1..798
                     /locus_tag="PKN92_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ47660.1"
                     /translation="LLPVHIDQSQLNQIVTNIAVNAADALNGKGVVHVSTDTVQLEDE
                     PLLVRSSLPSGQYVKIIIQDTGPGIDPAIESKILEPFFTTKAPGKGTGMGLAVVFGIV
                     KQNNGFLDIRKTPGEGAAFIIYLPACTDRAVAESEKRSASVDGVTKTVCIIESEPDLR
                     DLLVEILKKAGYTVLAASSFEDAKTLIALEHEVIDLFIINAVEFLADDAVLAEEISKH
                     CPQRKILLMSTLAKEEASVHLFDPEVCAIIEKPFSLDAFFRQVNDLI"
     gene            810..2726
                     /locus_tag="PKN92_02325"
     CDS             810..2726
                     /locus_tag="PKN92_02325"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNZ47661.1"
                     /translation="MPFIATPLLLCSLLFAATDAEIIADPKVEKSIEEALASAPGHPR
                     LLITARDLETLRKEIPADRNRNRILEVVVAEADKMLEKAPSERIKQGVRLLSVSREVL
                     RRVLFLSTAYLFTEDLKYAERAVLELRAAASFSDWNPSHFLDVAEMTTAFALGYDWLF
                     AQLSEEDKGLLQEAIVTKGLNPSFQGRAHWARRDNNWNQVCNGGMLFGALALAEHEPA
                     LAKKVIARALDGLPYAMATYQPDGLYVEGPSYWEYGTTYNVLAIAALESSLGTDFGLA
                     DFPGFKETGTFPLFMTGPTGDHFTFSDCGPKGQAFPAVYWFARRWQDSGIASLEHRWL
                     QKLLDGSTDYGGRFLPLMLFWWQGDTPAQPKQSLAWHGDGLNPIAVLRSSWESPDAVF
                     IAMKGGTPRANHGHMDVGSFILEADGIRWAVDVMGKGYGELEALGLSLWSMKQESDRW
                     KIFDYSNLSHNTLVVNGTGQIVGGDAPLVQFSDDGAFPFAVFDMTAVYQDQLAKAVRA
                     AALLPDGRVVIQDELVNKKEPSSVRWAISTPAVLEATGDTEGILHCRDRQLRMAVTAP
                     LSEKLQTLPEHAWQEWDKPRGHLRQVGFFMNLAPEEEVLCRVVFTPGSAADSADGILP
                     VMPVSRWGERAEQE"
     gene            2980..4689
                     /locus_tag="PKN92_02330"
     CDS             2980..4689
                     /locus_tag="PKN92_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ47662.1"
                     /translation="MEGVFPEGQNDNFQFVILGDKSSGGEDLWPLFDQAVDSINLLSP
                     DFVITTGDHIPGHMEERRQWDLEWAEYREHARRLQCPLIMTPGNHDISNTQCHQFWMD
                     DFGATYFQFTYKKCLFLVLNTEEERFDGRGPRWEAMMQFAEKALEDAKDCHHTFLFFH
                     KPMWDDPRFLRDWGQLLQWLGERPFTAVAGHEHYLSVSRQGSNLLIVMNATAAGVHES
                     EVLPFGGFHAFAPVTVRSGKVHVSVMEPNGIIHPVDIAPSSFRKKINREVVFPDADFP
                     DCSDPEFAETKSFALVKNPFEKPIAVRLNVTSLQACRWELKDPSALDINVKSGDAQTE
                     VSLLPGEEKRISLACRVPAERIGTPPLLEWEVQYEGQWLKKEIMYMEEEAALPLYPAH
                     CWKMAPAWQQTDPFIIGDINTAALPRDPEKANLWFVNELGPEKGYEKGVRYDGGQQWH
                     PAESCARGLVNLNGLLGTLDLAATFSSCRVFSPEAQNTHGFMYADNYAQLYLNGSLVE
                     EGQALRGPSPFVYVPLHLNAGWNVLSIKTVNNRGDWFLRFLVADAQNNLCFDSWQESE
                     RSS"
     gene            4934..6331
                     /locus_tag="PKN92_02335"
     CDS             4934..6331
                     /locus_tag="PKN92_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ47663.1"
                     /translation="MIDWMLSRPSILPALDPDFKPPVLAHRTYLQAVKDAGGGVPLVL
                     GLERGDGSLSRFETRVFEESDPRTEASKFYVERLVKFLLWQRGGWRVYVGGPKSIGEH
                     IQQCYQAGGSRAFDFEFMGVSIYDKAFTVVICDADEVPAQKENASALGRHLEGYRIGF
                     DLGGSDRKVSAVVNGEAIYSEEVMWNPIQTADPAYHYNEVMTAIKTAASKMPRLDAIG
                     GSAAGVYVDNRARIASLFRAVPKERYHEVHDLFPRISREMGVPLVMVNDGEVTALAGS
                     MSLEDNGVLGIAMGTSEAGGYVNLDGNITGWLNELAFCPVDYNPAAPVDEWSKDYGVG
                     ALYFSQQCAFRLAPKVGISLPDNILNAEKLKIIQDKMEAGEEGALKIWQSMGVYLGYT
                     LAHYADFYDIRHALILGRVTSGKGGDLLLSGAQEVLEKEFPALAESMCVQLPDEKSRR
                     VGQSIAAASLPDISK"
     gene            6385..6891
                     /gene="folK"
                     /locus_tag="PKN92_02340"
     CDS             6385..6891
                     /gene="folK"
                     /locus_tag="PKN92_02340"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNZ47664.1"
                     /translation="MESVSSVLLCLGSNLGDRLTMLKRAIQLLGGHASVRVVRSSSVY
                     ESEPWGLTDQPKYFNIVVEIETVLTPLELLKTVKDVEQQLGRQPSEKWGPRSIDIDLI
                     LWGSHCMRTPELTIPHQLFRDRAFVLAPLAEIAPAAVDPESGMTVTALAGRVDAGGLV
                     QIADPLFS"
     gene            6957..7748
                     /gene="panB"
                     /locus_tag="PKN92_02345"
     CDS             6957..7748
                     /gene="panB"
                     /locus_tag="PKN92_02345"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNZ47665.1"
                     /translation="MLVTTQTFRKKKEAGEKIALLTAYDYPTAMLMDESGVDAVLVGD
                     SLGMAVMGMENTLNVTMDMMVHHVKMVRAGLSRAMLIADMPYLSYQINTAEAIRNAGR
                     LIAEGGAQAVKLEGSARFFGEAIAGLGRAGIPVMGHLGLTPQFINQLGGYKIQGRTEE
                     QARQLQTEALELQKAGCFALVLECVPAELAMQISNNLSIPTIGIGAGNGCDGQVLVWY
                     DMAGWGRTKFTKNFKDVRSLMLDAFTAYVEEVRRGEFPGKEHSFE"
     gene            7745..8599
                     /gene="panC"
                     /locus_tag="PKN92_02350"
     CDS             7745..8599
                     /gene="panC"
                     /locus_tag="PKN92_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNZ47666.1"
                     /translation="MNIINDPQEMRRCADHLRAEGKTIGLVPTMGALHAGHMSLVHAA
                     LRENDVAVVSIFVNPTQFAPHEDFGKYPRTFQSDIEQLKAAGVSYVYAPSAESMYPED
                     FVTYVEIGKVTEGLCGGSRPQFFRGVATVVMKLFHAVKPHRAYFGQKDVQQCAVIKRM
                     VRDMDMGIDIIILPVVREPDGLAMSSRNAYLSPEDRRQALAISRALFEAGERMCGGER
                     DSTELIRGVRECMAALQIDYVSLVDAESMQEVCRIEGKVALAAAAVLPTARLIDNIIY
                     DPVENRVF"
     gene            8660..9019
                     /locus_tag="PKN92_02355"
     CDS             8660..9019
                     /locus_tag="PKN92_02355"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNZ47667.1"
                     /translation="MLLTLMKSKIHRATITEANLNYQGSLTLDRDLMDAAHILPYEQV
                     HVLNINTGDRFVTYAIEGEPGSGVVCLNGAAARLGHPGDLIIAISYAQMGEEEALNYV
                     PYTVHVDENNKIRSSSR"
     gene            9239..10942
                     /locus_tag="PKN92_02360"
     CDS             9239..10942
                     /locus_tag="PKN92_02360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNZ47668.1"
                     /translation="MNFFTKTAIQLHTLKWLMTWRKHNNHCVVPVKDNPKFMTPWQAA
                     GIIPPGAVMGTSGLGGNQRCSAMYWAMRERFQAEGTPKDLTIVSVGGQGGRGKIPGTL
                     EEIALKGLIRRFITGHSETFKAQLKMADAGDLELQILPQGTLTLLLKAQGEGVDHVIN
                     RTGVGTFLDPRVGRGTPLVPENAEQLVEVLEDGSFKYHLPPIDVAVFNAPAADRKGNI
                     YFKDCAVLCESLEIARAAKKNGGKVIVNVGLIVEEGYDEIGIAADDVDAVVYWRDTEQ
                     SATIKHRAKWDCLTLNSRKTEKEGLARVKVINAFLKVTPRRKAVDSLLARLGTYIFVQ
                     NAKKGDHVDIGVGLPEEVSRLLYENGVLKDLTMINESGVFGGVAAPGVFFGAAVNPTE
                     IVSSAEAFCRIYKRLDWAILGALQIDSSGNVNVSKRGDGARNYVGPGGFIDLITNAKS
                     LLFCSAWGDRAQFELTPDTIKVTDSGKAKFVDVVDEITFNGSEALRHGKKVFYITHVG
                     AFQLTDRGMELIYIMPGIDLQKDILDATSMKIVLPESGEPELVPGFVMTGKGFNMTLP
                     D"
     gene            11055..11387
                     /locus_tag="PKN92_02365"
     CDS             11055..11387
                     /locus_tag="PKN92_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47669.1"
                     /translation="MLKDKIVRVRLRKFFNEQKPTSYVGKCVVFTNDWIVVEGFGIML
                     TRASKNGAQIDARPSQTAIPSSNIESIRVLPDDFAYGRLQLSTEGQQLVIVVPNAQPC
                     VIGEIGEG"
     gene            complement(11396..12259)
                     /locus_tag="PKN92_02370"
     CDS             complement(11396..12259)
                     /locus_tag="PKN92_02370"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ47670.1"
                     /translation="MTAQRISLLITAVFLVPLSSWSQTVDSIIEGNFSIRLGGHRSGR
                     LEWPENTVTAIRAFSDRWKQALIEVDVQTTADGKLILLHDLDVDSVTNGSGVAMLMQF
                     EALRALDAGWQFTRDRKEYPYRGQGVVIPTLQEALAAAPDHPFLIEMKDGAEVAKATA
                     AAIREACAEQRCFVATVNPVTLAEFKSEAPEIATCYDVLDAAEAIAAMRNGAWDSYTP
                     PHRMFVLSLELQKQFDLSWEELRRFQERGILLCFFTVNREDEMKHLESHGVDCILTDY
                     PARLAAVLGYE"
     gene            complement(12348..13016)
                     /locus_tag="PKN92_02375"
     CDS             complement(12348..13016)
                     /locus_tag="PKN92_02375"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNZ47671.1"
                     /translation="MSALLLPVLGYIGALLFYGRFYLQWFVSERQRKSVIPIGFWYMS
                     GIGSIFLLLYGTLWLSSPVGALSHCFNSLIYARNLVHIWRERGLLTPRRSLLFQGAVL
                     LMIAAGMLLVAWTWLNEYESTRDIPVVDQARNWIWIAVGVAGQGLFALRFLIQWAVTE
                     LKRKSIVPASFWYLSLVASLLLMSSHLQRLEWLYATGIASTIVVYLRNIYWIHKAPER
                     SSAE"
     gene            complement(13105..15549)
                     /locus_tag="PKN92_02380"
     CDS             complement(13105..15549)
                     /locus_tag="PKN92_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ47672.1"
                     /translation="MSKVIVIVGGVAGGMSCAARIRRLDEEATIIVYEKGSAVSFANC
                     GMPYYVGGIIADRANMIVQSRETLRGRYNLEINVRHEVTKILPEEKEVEVRNLETGET
                     YRRSYDTLVLAPGAEPIRPPIPGIDSEHVLTLNSLDDMDKIKAKAAVSQRACIIGAGF
                     IGLELTENLRHLGLNVTLVEMLDQILPPLDKEMTAPLLQELRLNGVEVRLGVRAEKID
                     AHGVTLSDGTNIPADLICLSAGVRPRSELARDAGLELAASGHIVVDAQMRTSNPSIFA
                     AGDAVQVWDRATQAPATLPLAGPANRQGRIAADVICGREAAYPGVLGTSIVQVFNQQA
                     ACTGWSEKKLKNESVPYFSVYIHPMQHVQYYPNAVPVSIKGLFSPEGKLLGAQVIGPE
                     EVCRTIDTLAMALANGMTAADLEHSELAYAPQFGAAKSGINMLGFTANNVVKKDLEQI
                     DCRDLTKEHFLLDVRMPDETACGIIPGAVIIPLDELRNRHNELPRDRTIALYCAVGLR
                     GYVAYRQLKNYGFNVININGGYRTWAWSHGAAPAAGQNCSPCSGAIPGKSPAPAGKVT
                     QLDVCGLQCPGPITQVKRLMDGMNPGDTLEVTSTDVGFIADIPAWCKSTGNTLLEARP
                     DGNRYLARLVKGGTAPAASTAPAASGSASAATSGKNSKTIICFSDDLDRVMATFVIAN
                     GAAAMNSEVTIFFTFWGLNVLKKSRPPSVKKGFLDRMFGMMMPKGPEKLKLSKMHMAG
                     MGSKMMRYVMANKRVMAPEELVAAARAAGVRLVACSMSMDVMGIHKDELIDGIEIGGV
                     GAYLGTAEQSNVNLFI"
     gene            complement(15805..17373)
                     /locus_tag="PKN92_02385"
     CDS             complement(15805..17373)
                     /locus_tag="PKN92_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47673.1"
                     /translation="MSVNPLQGIPRCDHTKLRRSQRAFRMTVTLLLVLTAAWWFSESY
                     LRYDHSETQYRMALTLQRAQARPILRTVVERESKEKDTPSALYLEALAEVEEAEKLLP
                     AYARAYQSNPKSESLLINYGCHLYEMGQYGEARERFREAGINPPRNLLPRYLESAALL
                     ASSEGKEERSNALALIARANASGDPLLVPEPLWHDSLPRHGKGYLHKYYMTADRIFET
                     IHNSTLLFCAALDREATQENAAELNSHLEKIEAMSMRLLGMNKGDSPPALRQLEHALQ
                     IQLAVHQQRGRLADNFGLPAEPEYAERSQKLLNLLKELDQFNASGDALAQKYARRLLI
                     PFVLILESVVLFTLFYFALLLLRRFLKPCTHLPFLPVTGTGKKLLFTGFTALFLLLLL
                     MPALYSTNREYVWEYPMQLLGEILCVGLLLFFGIYYPLSTLRAFLKDARHHELQEVWV
                     EQKRLQLSIFSCLALRYAGFLLGGLIICVCLWLNFTRIFYSAYPFQSALLPSGLEADI
                     GALVNSIKDQLKNF"
     gene            complement(17370..18758)
                     /locus_tag="PKN92_02390"
     CDS             complement(17370..18758)
                     /locus_tag="PKN92_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNZ47674.1"
                     /translation="MKEIQAPVYANIEINISRAKSSAVSAAHGITPAEFAAIEERVLA
                     AHKILQQERKEGKYGFYDLYKDKETLADVKKAAAHFSSFGYENLVVLGIGGSALGITA
                     LQTALNPPYFNTLSKKRRKGAPRLFVMDNIDPDTFRAMLRLCPPKKTLFNVISKSGET
                     AETMSQLMIVVALLEKAVGAKAMKKHLVITTSPTGKGAPKSLLHPVAKNYKLTSFSIP
                     LNVGGRFSVFSPVGMFPAAMMGMDVDAMMAGCAAMDTLCSRASLMENPAYLRAAIQYL
                     MDVNKGKTMSVMMPYADALRDIADWYRQLWAESLGKQKDLDGNDVFTGQTPIKALGAT
                     DQHSQVQLYREGPNDKIFNILEVRRFNTSLRIPDTLSSIKELDYLRNKSMNKLMAAEL
                     RGTMDALKASHRPVVRIILPRLNAYTVAQVLYLLEVETAMAGRLYHVNTFNQPGVEEG
                     KKIARRLMGGEA"
     gene            complement(18946..21120)
                     /locus_tag="PKN92_02395"
     CDS             complement(18946..21120)
                     /locus_tag="PKN92_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNZ47675.1"
                     /translation="MRNCVTGLLVALLCCTAVFAADEVPAKVGVFPQEIAKNYSVADG
                     LPDQKVLSLHQDETGIVAVTAAGACRLEGATWKPVRDFTPQRFVLVPDGDAYTLAPAE
                     AAPGSGAVIVRSFAGDAKAPVAIAAAEGLFLRNDQGVYEKSPACDGQGRLWGACDVRG
                     VTLDSDGNLWVAMLAGLACRTADGWKFYTGHEGLPYNDFTSIAAGPDGAVWFGTTKGI
                     VRFKNGTWHYRQGRRWLPDDHVNDLLVDQAGNAWAATQHGIGWIGFTPMTLAEKAAFY
                     EEELDKYIKRTPFGYTSEVKLEKAGDRSTVVYTDSDNDGLWTSMYGAGECFAYGATRD
                     PEAKKRADKAFEALRFLQTVPQTGDHRPPKGYVARTILPTDGKDPNEGRIERDTYHRD
                     NHDVLWKVYEPRWPKSGDGKWYWKSDTSSDELDGHFFFYPAYYDLVAETKEEKEAVQQ
                     VIRDLMDHLIDHDFNLVDFDGTPTRWSIYNPDSLNKDPLWWEERGLKSLSMLSYLTAT
                     EHVTGDPKYGKIANELIEKHHFRTNAMIYKIHFGPGSGNHSDDEMAFMCYYNLLKYSQ
                     DKALKHEILYSFFSAWINEWPEMNPLFNFMYAVFGRNAKHTNPWDTYDISPWEGWLDD
                     SVETLKDFPLDRVAWDHHNSHRLDIVLLPRQQATEPYESEVPQRGHRVNGKVLPVSER
                     YFNHWNHDPWNLNYGGKGHTLGSGTVFLLPYYMGLYHGFIDN"
     gene            complement(21294..22628)
                     /locus_tag="PKN92_02400"
     CDS             complement(21294..22628)
                     /locus_tag="PKN92_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ47676.1"
                     /translation="MKKALLIAVTASLISTLLLVPLFSGCAPATPGGSKDRSFKPLDP
                     DFAVFWDRQTTESAQLLQDLAAEFNRSWQGIPIKVERAGNYTDIFRKVSASIRAGVLP
                     AMAVAYESMTNDYINAGAAVDLEPLLADEEAGLSAEALADYIPGALENNRFANHDNHL
                     YAFPFAKSVLILYYNKKVMQDAGITAPPATWDDFINQCRQIKAKTGKYAHGVHADCST
                     VNGMIFSQGGDVLKDGKCLYDSPEALAVFRIYEILAKENLAWLVSPSSYEDNVALSKD
                     EIAFVMRSSSSFSDMKLLMEGDTERWGIAPIPQKYPAKPGTTLYGPNVCIFNTTEAHI
                     QAAWAFMKFLTSPEVSVRWSLETGYLPVRRSTLHQPELQQHWEKWECNKVPYDCLAFA
                     RTEPNAPGWQEVRDLATRAVSEIMAGVTTAEKAAAALQSGAERILARNARPQ"
     gene            complement(22631..23446)
                     /locus_tag="PKN92_02405"
     CDS             complement(22631..23446)
                     /locus_tag="PKN92_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNZ47677.1"
                     /translation="MKFNRLLLYLFLCCGAFLALYPFLWMLGASFKTLNEASTPSLNL
                     LPKVWQWSNVTETFRAAPFGLYFFNSFLVAGSVCLSVCATSLMAGYAFARLQFKGRQL
                     LFFIILGSMMVPFELTFIPNFVLISRLGWYNSYAALIVPWCVSAFSIFLMRQAFLALP
                     RDFYDAAVLDGCGHLRFLVRIGIPLVMPSLVTVALFAFLGSYNALLWPLIVTADDRCR
                     VVQIGLVAFYSDAGARLNLLMCASSIIILPTVLIYFVAQRYFVQTTMGTGIKG"
     gene            complement(23443..24477)
                     /locus_tag="PKN92_02410"
     CDS             complement(23443..24477)
                     /locus_tag="PKN92_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNZ47678.1"
                     /translation="MTQFKKRLQIFLFPFVLLAVAGDRCLGMLDDGLDALRRRGLGDW
                     PAALLLLLPAVILLSLFSLYPMGASLQMSFFGGRHGEGDFVGLANYTEALNNPDFRRS
                     FLVTLYYVLGVVPLTVSLSFIIAFGLFKITRGRGLLRSLYFLPYVTSAVAAAMVWRSL
                     FHPQQGVVNLLFSYLGFSPQNWLLEPRGILHLLSHGWIPADLGPSLGLCCIMLFDIWH
                     GSGFMIAVFLAGLSVIPRELEEAARMDGAGEWRLMWHVTLPLLSPTIFFLSIVGVIKA
                     FQAFNSFYALTHAGGLPDTQNLILYVYAQFYQYGYWGYGAAVATLLVLVIVALTLLQW
                     RVLGRKVFYS"
     gene            complement(24545..25519)
                     /locus_tag="PKN92_02415"
     CDS             complement(24545..25519)
                     /locus_tag="PKN92_02415"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HNZ47679.1"
                     /translation="MSKVVIGVDLGGTNIKAAVVSEDKKIIVQGDCPTGAAAGPGPVM
                     DLMAGFVKDLIASAGLTMADVLAAGFGAPGPMNWRSGVVFSPPNLPGWKDVPLADEMK
                     ARLGVPCYVDNDANVACYGEYWMGAGQGAESIVVFTLGTGLGGGIVVFGELLRGIDGT
                     AAELGHLKVQKDGRLCGCGSKGCLETYASVTGMLRTAKEGWDGADTSLKALCDNDPGK
                     LTGKMIYEAAMSGDAFARDVFTETAVWIGLGAASMVNALNPERIILCGAMSKSGDLLY
                     ETVRKTVKENAFEVPAARCQILPAGLGNDSGVLGSAGCALARHHAASC"
     gene            complement(25516..27003)
                     /gene="gltX"
                     /locus_tag="PKN92_02420"
     CDS             complement(25516..27003)
                     /gene="gltX"
                     /locus_tag="PKN92_02420"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNZ47680.1"
                     /translation="MTTSAVRCRVAPSPSGFLHIGTAKTALYNWLFARSNGGTFILRL
                     EDTDAERTEEQYVQAMCEGFKWLGIEWDEGPEFGDEPEKGAFGPYRQSQRLQLYSEEA
                     ARLVAEGKAYRCYCTKEELDAQREQAALEKRPPRYSGKCRNLTPEEIAAKGDTPYAIR
                     FRVPEGETVIDDIIQGPVRIQNKEYDDFIIVKSNGDAIFHLAVVVDDGLMKITHVIRG
                     DDHLTNAGRHVMLFNALGYPLPKFAHHPLIHDEQGRKYSKRLHGANVLDWRKDGYLPE
                     TMINYISLLGWTSEEEGREFYTLDELKQLFTLKRLTKSPARFDRKKLDWLNGQHIRML
                     SPDALCDRVLPILREQGFPVDERSRDWLVEMAAICQEKIPTLNHIIPYTDFFFAAPTA
                     YDEKGDRKFFRQDTSLQALEAALECMRQADEWTVTALKESYEAAAEQKELKIGALVNG
                     TRLALTGKTVGPGLYELVKLLGREESVQRMEAALNYIKTSGEGTA"
     gene            complement(27182..28459)
                     /locus_tag="PKN92_02425"
     CDS             complement(27182..28459)
                     /locus_tag="PKN92_02425"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNZ47681.1"
                     /translation="MTTAKLIYNGQEYDIPVIVGTENEGALDIRKLRDTTGLITYDPG
                     YANTGSCQSAITFIDGDRGILRYRGYPIEELAGSVRFSAAAYLMIYGRMPSEAEFMEW
                     RKQLTLNSLIHASQVNFFDNYSAHAHPMNILSAMVSSISSFYPHIGGENDNMDQHIQR
                     LIGQVKTIAAFSYRKSVGLPYIYPRTDHSYSANFLRMMFASPAEEYVVPKVMENALDM
                     LLILHMDHEQNCSTSTVRMVASSMSNIYASVSAGINALWGRLHGGANEAVLNMLQRIA
                     DDGSDYKKYVELAKRKDDPFKLMGFGHRVYKNFDPRSKILKKAVDDVLTEMGIQDPLL
                     DIAKNLEEVALKDDFFIERKLYPNVDFYSGILYRALGIPTEMFTVLFAIGRMPGWIAH
                     WLEMSKDPDLRIQRPRQVYIGENLRHYEKTIYP"
     gene            28878..30512
                     /locus_tag="PKN92_02430"
     CDS             28878..30512
                     /locus_tag="PKN92_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47682.1"
                     /translation="MKKVLLGILTVLLIFVLLIGLFLFSRTRGPHRSYELDFVKIGSA
                     ADAGALLVGVSKVDITPDLSLYDTYYDADNNNKFNPAKKDMNRLERLALRFGLIKEGQ
                     DTWTDSNKNGRFDPVWMAGFSPNRPAKGVHDPIWARAIAFRNNGVTVAMVTLDAIGIF
                     HDKIIDIRKMIDPSLNVDHVIVTSMHNHETPDTMGNWSGLVPRPANFDQRHMNNVKMA
                     CKTAVEQAVRSMVPADMYLKTIMIEPEGLVNDSRKPIVIDRQICTARFNRAGTDTTLA
                     TLVSWGNHPEAAGSDNPLITSDFSGYWRDGVEKGVSDPNGEEGIGGMCLYFQGMVGGL
                     MTQLHTTVPHRNGVDMIRENGFAKTEALGDNLALITLRGLKEPDVVKSEDVRVGVAAK
                     TIYVPMAGLFKYAIMLGLVHPGTYWKDGAITSKTEVNVVRVGDLEIITVPGELYPEIA
                     EGGVEAKPGRDFDIPPLEVPPLRKEMRGKVNMVFGLANDEIGYIVPKSQWDVEPPFVY
                     EKAQYGEENSGGPDVAPTIHRESLALMRRFAEAWDN"
     gene            complement(30614..31429)
                     /locus_tag="PKN92_02435"
     CDS             complement(30614..31429)
                     /locus_tag="PKN92_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNZ47683.1"
                     /translation="MRTLISKTMTVLFLALCPLILMPCGQAEDDISHFFSVFKQKRDG
                     IESLEAKFEQTTILPEEKLITHGVLRYSKPRRILFQTEDPEHALLMDGRQGYEYDADI
                     RQMTMFYVEDHPRANIFFLGFDNDTEALRAAYDLTLLVSDDPRGRQGIRIRPRTGSEE
                     AEYFLEVNLYLRESDYFPYRIHIVNDQESELYIDVKEIRTLNAQDHETPRIFLPEGTT
                     IVAEDRVVDVVGEGGRFVPAGNTGTEQSVKDLPPLDAGEANESEKASPIVPSE"
     gene            complement(31410..32141)
                     /locus_tag="PKN92_02440"
     CDS             complement(31410..32141)
                     /locus_tag="PKN92_02440"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNZ47684.1"
                     /translation="MTRQKSDPANWAHTDGQAQADYQEELIMTEEREFQEVQPGHAPL
                     LTAEQLYSAQISTRPMGYSKVQVDMLLEEAAATIESLRLEKEGLAATAAALEQELHKY
                     REMESSLRASLLSSQKMGESMIASAKLQADALVEEARIAKERAVFKMEQLTPALQGEI
                     QRLTAARDQLRQDLSAILKAHEALIETIPRAETKEQAASSEKNTAAEKSKTGKNKTKT
                     SAGSSVAAGQDGENSREEDNAYIDL"
     gene            complement(32098..32913)
                     /gene="proC"
                     /locus_tag="PKN92_02445"
     CDS             complement(32098..32913)
                     /gene="proC"
                     /locus_tag="PKN92_02445"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNZ47685.1"
                     /translation="MATLSGDFGFLGYGNMGAAIAAGMVAQGTVTPRQISVYDPDTEK
                     VRAASALGFRCVASAEMLAQHCDTLLLAVKPQMLEEALAPVCAAARPDACVLSVIAGI
                     SIARLQVLFGAEARILRVMPNTPALAGAGAAAVAAGTNCTKEDIARGRAVFESVGIAE
                     VVPEAWLDGVTALSGSGPAYFFYLTECLINAAVRRGIPESTAAHLAAQTLYGAGKLLI
                     ESGESPATLREKVTSKGGTTFAALERMRGRDLEGLVQEAFDAAEKRSRELGAH"
     gene            complement(32938..34722)
                     /gene="dnaX"
                     /locus_tag="PKN92_02450"
     CDS             complement(32938..34722)
                     /gene="dnaX"
                     /locus_tag="PKN92_02450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNZ47686.1"
                     /translation="MSSSSYVAIARKWRPQTFDEVVGQEHITRTLKNALQSGRIHHAF
                     LFIGSRGIGKTTTARILAKALNCESSDKPTMNPCGTCGNCLSITQGTNIDVIEIDGAS
                     NNSVDDVRDIRETIRMVPSSSRYKIYIIDEVHQLSLPAFNALLKTLEEPPPHAIFVLA
                     TTEAHKIPATVISRCQRYDFRRVTIHNLVALLRTIVEKEGLAASDDALYAIARVAEGG
                     VRDAESILDKLITYSETSITFEDVFDVLGLVPWKAFHDLCDAMLQGDIATQLEIVDDV
                     AATGKDLSQFVEDLLRYYRNLLIYRTAGAREALLRLPEAEMEAIRIRSARFPVVKLIR
                     LIEAFSDLCNNFDSQLAQRTALEAMLIRSTRVDVEVSLDTVMEKLVHLGAGGILPPEP
                     GRREIPDSRPAEETAPVPAPAEKPSAPTERKMPRPPENPPEAGSPSGTSTIRQKEERI
                     TLGADTLDSQWRQLVLDAGKTSLSLGLWLGRAHPLAILEDTGELELELEPGAVQAHAF
                     FEDEENRKSLHAFLQGRTNNIRSVRFTLAAPTVPRISRKKKPAETMKTSTDNLEQDEK
                     IAAVLHEFKGRIVRVIPGTEAGDAPSSQ"
     gene            complement(34786..35054)
                     /gene="ffs"
                     /locus_tag="PKN92_02455"
     ncRNA           complement(34786..35054)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKN92_02455"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            35372..35812
                     /locus_tag="PKN92_02460"
     CDS             35372..35812
                     /locus_tag="PKN92_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNZ47687.1"
                     /translation="MVQKKNRNRDGFTLLELMISVAIFAIILSIAVPNLLRSRLQSNE
                     VATIQNLRSINSAQGAYNSAKKVYATTFEDLCNVALPFLPSTFLTQPMAGYTITLAGE
                     EDLYTVVAEPSEWGVTGNRGFYTDASGIIRFAISEPADEGSPPL"
     gene            complement(35987..36063)
                     /locus_tag="PKN92_02465"
     tRNA            complement(35987..36063)
                     /locus_tag="PKN92_02465"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36027..36029),aa:His,seq:gtg)
BASE COUNT         8416 a         9464 c         9879 g         8589 t
ORIGIN      
        1 cttctgcccg tgcatattga ccagagccag ctgaaccaga ttgtgaccaa tatagcagtc
       61 aatgctgcag acgcactgaa cggcaaaggc gtcgtgcatg tgtctacaga taccgttcag
      121 ctggaagacg agccgcttct ggtgcgctca agtctgccgt ccgggcagta tgtaaaaata
      181 attattcagg atacaggacc cgggatcgat cctgcgattg agtctaaaat actggagccc
      241 tttttcacga ccaaagctcc tggtaaagga acgggcatgg ggcttgccgt cgtgtttggc
      301 attgtcaaac agaacaatgg gtttctggat attcgcaaga cgccggggga aggagcggct
      361 tttatcattt atttgcctgc ctgtactgac agagcggtgg ctgaatcgga aaagagatct
      421 gcctctgttg acggggtgac gaaaacggtc tgcatcatag aaagcgagcc ggatctgcgt
      481 gacttattgg tggaaatact caaaaaagcc ggatatacgg tgcttgcggc ttcttctttc
      541 gaagatgcaa agacgctgat agcgctggaa catgaggtaa tagacctttt tattataaat
      601 gcggtagagt ttttagctga cgatgcagta ttggcagaag agatttccaa acactgccct
      661 cagcggaaaa tacttctcat gtccacgctc gcgaaggagg aagcaagcgt gcatcttttt
      721 gatcccgaag tctgtgcgat aatagaaaaa cccttttctc tggatgcttt tttcagacag
      781 gtaaacgatt taatataaag agatgtgaaa tgccttttat cgcaaccccc ttgctgctgt
      841 gcagcctgtt gttcgctgcc accgatgccg agatcattgc agatccgaag gtggaaaagt
      901 ctatagaaga ggcactggct tcggcgccag gtcatccgcg tctgctcatt actgcccgtg
      961 atctggagac tttgcggaaa gaaatccccg ctgacagaaa cagaaacagg attcttgagg
     1021 tagtcgtggc ggaagcggac aagatgctgg agaaagcgcc gtcggaacgt ataaagcagg
     1081 gggtcaggct tttgtctgtg tcccgcgaag tgctcaggcg tgtgcttttt ctcagtacgg
     1141 cctatttgtt tacagaggat ctgaaatatg cggagcgggc agtacttgag ctgcgcgctg
     1201 ccgcttcatt ttccgattgg aatccgtcgc actttctgga cgttgctgaa atgacgaccg
     1261 cttttgcgct gggttatgac tggcttttcg cacagctttc tgaagaagat aaggggctgc
     1321 tgcaggaagc gattgtcact aagggactga atccctcctt tcagggacgt gctcattggg
     1381 cacggcggga caacaattgg aatcaggtat gtaacggcgg catgcttttc ggtgcgctgg
     1441 cactggcgga gcatgagccg gctctggcaa aaaaagtgat tgcccgggct cttgacgggc
     1501 ttccctatgc catggcgacc taccagcccg acggccttta tgtggaaggc ccttcctact
     1561 gggaatacgg cactacctac aatgtgcttg ctattgccgc cctggaatca tcactgggaa
     1621 cagatttcgg tctggcggat tttccgggat ttaaagaaac aggtactttt ccgcttttta
     1681 tgactggacc taccggtgac catttcacat tcagtgactg cggccccaaa ggtcaggcgt
     1741 ttccagctgt gtactggttt gcccgccgct ggcaggattc cggcattgcg tctctggagc
     1801 atcggtggct ccagaaactg ctggacggca gcaccgatta cggtggccgt tttcttcccc
     1861 tgatgctgtt ctggtggcag ggtgatacgc ctgctcagcc gaagcagtct ttggcatggc
     1921 atggggatgg attgaatccc attgcagttc tccgatcttc ctgggagagc ccggatgctg
     1981 ttttcattgc gatgaaaggc ggcacgcccc gggcaaacca tggccatatg gacgtaggtt
     2041 cctttatact ggaagcggac ggtatccggt gggcggttga tgttatggga aaaggctacg
     2101 gcgaactgga ggcgctgggc ttgagcctct ggtccatgaa acaggaatcg gatcgctgga
     2161 aaatttttga ttacagcaac ctcagccata atacgctggt agtaaacggc accgggcaga
     2221 ttgtgggcgg agatgcgcct cttgtccagt tctccgatga tggggcgttt ccttttgcgg
     2281 tctttgacat gacggcggta tatcaggatc aacttgctaa agcggttcgg gcagcagccc
     2341 ttttgcctga tggacgggtg gtgattcagg atgaacttgt aaacaaaaaa gagccctctt
     2401 ctgtccgttg ggcgatcagc acgcctgcag tattggaagc gacaggggat acggaaggaa
     2461 tactgcactg ccgtgatcgc cagctccgta tggctgtgac cgcccctttg tcggagaaac
     2521 tgcagacttt gccggagcat gcctggcagg aatgggacaa gccccggggg catcttcgac
     2581 aagtcggctt ttttatgaat ctggcaccgg aagaagaggt gctttgcaga gtggttttta
     2641 ccccgggctc tgctgccgac agtgccgacg gcatcctccc tgtgatgcct gtgtcccgat
     2701 ggggcgagag agcagaacag gaatagcccc gggaataatc tgcctccttt ccggcttgtc
     2761 ctgaaagcat aaaccacttc ttttaatcat aaccctttgc ctgctaccat gaaagccagg
     2821 ctatacctgt tctttttaac agatttccgt aggagaattt gagtgtttat cataccccgt
     2881 tttcgagtgt tttttggttt ctcatctttt ttactctttt tttgccttgc tgcggctatc
     2941 tgtgctgacg ttcccgtttt cgttcctgaa aaaattccga tggaaggagt ttttccggaa
     3001 gggcagaacg ataactttca attcgtaata ctgggtgaca aaagcagcgg tggcgaagat
     3061 ctctggcctt tgtttgatca ggccgtggat tcgatcaatc ttttgtcccc ggactttgtg
     3121 attaccacgg gcgaccatat ccccgggcac atggaagaac gcaggcagtg ggatctggaa
     3181 tgggcagaat atagagagca tgcccgtcgc ctgcagtgtc ctctgattat gacaccgggc
     3241 aaccacgata tttccaatac gcaatgccac cagttctgga tggacgattt cggcgccacc
     3301 tattttcagt tcacttataa aaagtgcctc tttcttgttt taaatacgga ggaagaacgc
     3361 tttgacggaa gaggtccgcg ctgggaggcg atgatgcaat tcgctgaaaa ggcactggaa
     3421 gatgcaaaag actgtcatca tacatttctg ttctttcata agcccatgtg ggatgatccc
     3481 cgctttctcc gggactgggg gcagctgctt caatggctgg gggaacgccc cttcacagcc
     3541 gttgccggtc atgaacacta tctttccgtg tcacggcagg gcagtaattt gctgatagtc
     3601 atgaatgcaa cggcggcggg agttcatgaa agcgaagtgc tgcccttcgg cggttttcat
     3661 gcttttgcac ctgtgacggt acgcagcggc aaggtgcatg tctctgtcat ggagccgaat
     3721 ggcattattc atcctgtgga catagcaccg tcttctttca gaaaaaagat aaacagggaa
     3781 gtggtctttc ctgacgcgga ttttcccgat tgttccgatc cggagtttgc tgagacaaag
     3841 tctttcgcgc tggtaaaaaa tccttttgaa aaaccgattg cggtacggct gaacgtgact
     3901 tcactgcagg cgtgtcgctg ggaattgaaa gatccttccg cactggatat taatgtgaag
     3961 agcggtgatg cgcagacgga agtgtctttg ctgcccggag aagaaaagcg gatttctctt
     4021 gcatgccggg tgcctgcgga gcgtatcggt acaccgcctc tcctggagtg ggaagtgcag
     4081 tatgagggac agtggctcaa aaaagaaatc atgtatatgg aagaggaggc tgctcttccc
     4141 ctgtacccgg cgcattgctg gaaaatggca ccggcgtggc agcagacaga tccgtttatc
     4201 ataggcgata ttaatacggc agcactgccc cgggatccgg aaaaggccaa tctgtggttt
     4261 gtaaatgaac tgggacctga aaaaggctat gaaaaaggag tgcgctatga cggcggtcag
     4321 cagtggcacc ctgctgaaag ctgtgcccgg gggcttgtca atttaaatgg tcttttaggc
     4381 acattggatc ttgccgcaac cttcagcagc tgccgtgtat tcagtcctga agcgcagaac
     4441 acccacggat tcatgtatgc ggacaactat gcacagctct acctgaatgg atccctcgtt
     4501 gaggaagggc aggcactgcg aggaccctcg ccttttgtct atgttcctct gcaccttaat
     4561 gccggctgga atgtcctctc catcaaaacg gtgaataacc ggggtgactg gttccttcgt
     4621 tttttggtgg cggacgctca gaacaacctg tgttttgaca gctggcagga atctgaacgc
     4681 agttcgtaac ttcttgccgg tatgcttttt tttaaactga aaaagggaag cagcaaaaaa
     4741 aacatttgtt tgcttatctc tttttagggt atcatctggt aacaccattc catcgcaacc
     4801 agcggtatgc agtctgctgg ttccgtatcg ctttcgtgaa atgactcagg cagggttgcc
     4861 tgccacggcg gaacggatgc tcttcaggca gactgaaaag tacttgaaac cactacttta
     4921 aaggaaacga gcaatgattg actggatgtt gtcacgcccc tctattcttc cggcattaga
     4981 ccctgatttc aagccgcctg tgcttgctca tcggacttat cttcaggcag tcaaagatgc
     5041 cggtggtggc gtgcctctgg ttctcgggct tgagcggggc gacggttctc tgtcacgttt
     5101 tgaaacccgg gtatttgagg agtcagatcc cagaacagaa gcttccaagt tttatgtgga
     5161 gaggcttgtc aaattcctgc tgtggcagcg tggcggctgg cgggtctatg tgggtggtcc
     5221 taagagcatt ggcgagcata tccaacagtg ttatcaggca ggcggcagcc gcgcttttga
     5281 ctttgaattt atgggcgtgt ctatttacga caaggctttt accgtggtta tctgcgacgc
     5341 ggatgaggtg cctgcccaga aagaaaacgc aagcgcgctc gggcggcatc tcgaaggtta
     5401 tcgtatcgga tttgatctgg gtggatcaga ccgaaaagtc agtgctgtag tgaatggcga
     5461 agcgatctac agtgaagagg tcatgtggaa tcctatccag actgccgacc ctgcgtatca
     5521 ctataatgaa gtgatgaccg ccatcaaaac ggcggcgtct aaaatgccgc gtctcgatgc
     5581 catcggcggc agtgctgcgg gcgtttatgt ggataaccgt gctcgcatcg cctcgctttt
     5641 cagggcagtg ccgaaagaac gctaccatga agtgcacgat cttttccccc gaatcagccg
     5701 ggaaatgggc gtgcctcttg tgatggtgaa tgatggcgaa gtgaccgcgc ttgccggatc
     5761 catgtctctg gaagacaacg gtgtgctcgg tatcgccatg ggaacaagtg aggcaggcgg
     5821 atatgtcaac ctggacggta atattaccgg ctggctgaac gaactcgctt tctgccccgt
     5881 agattataat cctgccgccc ctgtggatga atggtcaaaa gattacggcg taggtgctct
     5941 ttatttttcc cagcagtgcg ccttccgcct tgctcccaag gtaggcatta gcctgcccga
     6001 caatattctc aatgcggaaa agttgaaaat tattcaggac aagatggaag caggcgaaga
     6061 aggggcactg aaaatctggc agagcatggg cgtgtatctc gggtacacgc tggcacatta
     6121 tgcggacttc tacgacatcc ggcatgccct gattctggga cgggttacct ccggcaaagg
     6181 cggagacctt cttctgtccg gagcgcagga agtgctggaa aaagaattcc ccgcactggc
     6241 ggagtcaatg tgtgtgcagc ttcccgacga aaaaagccgc cgtgtcggac agtccatagc
     6301 cgcagccagc cttcccgata tttcaaagta atgagcgtct ttccggtccg gaggcaaagc
     6361 agctgagaaa ggaaatttct ttccatggaa agtgtttcat cagtactgct ttgcctcggt
     6421 tccaatctgg gtgaccgcct gacgatgctc aaaagagcta tccagcttct tggcgggcat
     6481 gcatcagtgc gtgtcgtgcg gagttcttct gtatacgaaa gtgagccctg gggcttgacg
     6541 gatcagccga aatactttaa tatagtagtg gagattgaga cggttctgac accgcttgaa
     6601 ctcctgaaaa cagtcaaaga tgtggaacag cagcttgggc gccaaccttc tgaaaagtgg
     6661 ggaccccgct ccatagacat tgatctcatt ctctgggggt ctcattgtat gcgcactccg
     6721 gagttgacga ttccacacca gttattcagg gatcgcgcat ttgttctggc cccgttggca
     6781 gaaatcgctc ctgccgccgt agatccggaa agcggtatga ctgttaccgc tcttgcaggc
     6841 agagttgatg ctggcgggtt ggtgcagata gcggatcctc ttttctcttg aaccgctccg
     6901 cttggagccc aaaagggacc gggacggaat cgtcacggtt caggaaagaa cgagccatgc
     6961 ttgtaacaac acaaactttc agaaagaaaa aagaggcggg ggaaaagatc gcactgctga
     7021 cggcgtacga ctacccgaca gcaatgctga tggatgaatc cggtgtagac gctgtgctgg
     7081 tgggcgattc gctgggcatg gcagtcatgg gtatggaaaa cacgctcaat gtgaccatgg
     7141 acatgatggt tcatcatgtg aaaatggtac gtgccgggct gagccgcgcc atgctcatag
     7201 cggatatgcc gtacctgtcc tatcagatca ataccgcaga agcgatccgg aatgccggac
     7261 gccttattgc ggaaggcgga gcccaggcag tgaagttgga agggtcggca aggtttttcg
     7321 gagaagccat cgccggactc ggccgtgcgg gcattcctgt catgggacat cttggactga
     7381 cccctcagtt tatcaatcag ctgggaggct acaaaatcca gggcagaacg gaggaacagg
     7441 cacgtcaact tcagacggaa gcgctggaat tgcagaaagc cggttgtttt gcgctggttc
     7501 ttgagtgtgt tcctgcagag ctggcgatgc agatttccaa caatctttct attcccacca
     7561 tcggcatcgg tgccggcaac ggctgtgacg ggcaggtgct cgtgtggtat gacatggcag
     7621 gctggggacg gactaaattt accaaaaact tcaaagatgt ccgttccctt atgcttgacg
     7681 catttacggc ttatgtcgaa gaagtgcgcc gcggagaatt tcccggtaag gaacacagct
     7741 tcgaatgaat atcatcaatg atcctcagga aatgcgacgc tgtgcggatc atctgcgtgc
     7801 agagggcaaa accatcggtc tcgttcccac gatgggggcg ctgcatgccg ggcatatgag
     7861 tcttgtgcat gctgcactcc gggaaaacga tgtggctgtt gtcagcattt ttgtcaatcc
     7921 gactcaattt gccccccatg aagatttcgg aaaatacccg cgcacttttc aaagcgacat
     7981 tgaacagttg aaagccgccg gggtctctta tgtttatgca ccgtccgcag aaagcatgta
     8041 tccggaagat ttcgtcactt atgtcgaaat cggaaaagtg actgaagggt tgtgcggcgg
     8101 aagcaggcct cagtttttcc gcggtgttgc gactgtggtg atgaaacttt ttcacgcagt
     8161 aaaaccgcat cgcgcctact tcggtcaaaa ggatgtccag cagtgcgctg tgataaagcg
     8221 catggttcgg gacatggaca tgggtattga tattattatc ctccctgttg tccgggagcc
     8281 cgacggtctg gcaatgagtt cccggaatgc ctacctgtcg cccgaagaca gacggcaggc
     8341 gctcgccatt tcccgggcat tgttcgaggc aggcgaacgg atgtgtggcg gagaacggga
     8401 cagtaccgaa cttattcggg gagttcgcga gtgtatggca gccctgcaga ttgactatgt
     8461 gtcccttgtc gatgcagagt ctatgcagga agtttgccgg atagaaggaa aagtagcgct
     8521 tgcagcggcc gcagtactgc ccactgcaag acttattgac aatattattt acgatcccgt
     8581 tgagaatcgt gtgttctgaa acatgaggac ggaagcgcga aggtcttgcc gctctgtacc
     8641 ttgaggatga aatctgaaaa tgttattaac cctgatgaaa agcaaaatac acagggcgac
     8701 gatcacggaa gcaaacctga attatcaggg aagcctgacg ctggatcgcg atctgatgga
     8761 cgcggcgcat atactgcctt atgagcaggt gcatgttttg aatattaaca cgggcgaccg
     8821 gtttgttacc tatgccattg agggagaacc gggcagcggt gtggtttgcc tgaatggcgc
     8881 tgccgccaga ctcggacatc ccggagacct catcatcgcc atcagttatg cgcagatggg
     8941 agaagaagag gcgctgaatt acgttcctta tacggtgcat gtggatgaaa acaacaagat
     9001 ccgcagcagc agccggtaag ggaaacgttt tcaactccct tcgcagatgc tcttttttgt
     9061 gtgtctgtct ttcttccggt attggctttt cacggccggc ttccgttata cttgatgatg
     9121 tgtgtgttta caggcacttt gcggggcggg caggttcttt gtcgagacaa gcaaaacagc
     9181 attcttcgaa gcatttcagc gcttctctga tgtggtccaa ccaaacagga taaatcaaat
     9241 gaacttcttc accaaaaccg ccattcaact gcatacgctc aaatggctga tgacctggag
     9301 aaaacataac aaccactgtg tggttccagt gaaggataat cccaagttca tgaccccgtg
     9361 gcaagcggcg ggcattattc cgcccggggc cgtgatgggc acttccggtc tgggtggcaa
     9421 tcagcgctgt tccgccatgt actgggcgat gcgcgagcgt tttcaggcag aaggcacacc
     9481 gaaagatctg actattgtca gtgttggcgg ccagggcggt cgtggaaaaa tcccgggaac
     9541 gctggaagaa attgcactca aaggcttaat caggcgtttt ataacagggc actccgaaac
     9601 ttttaaggcg cagcttaaaa tggctgatgc cggggatctg gaacttcaga ttttgccgca
     9661 gggaaccctg acccttcttc tgaaggccca gggtgaagga gtggatcatg tgattaaccg
     9721 gacaggtgtc ggcacttttc tggatccccg tgtcggtcgc ggaacccctc ttgtgcctga
     9781 aaacgcagag caactggtcg aagttcttga agacgggtcc ttcaagtatc atctgccgcc
     9841 cattgatgtg gcggtgttta atgcgccagc ggcagaccgc aaaggtaata tttatttcaa
     9901 ggactgcgcg gtgctttgcg aaagcctgga aattgcccgg gcagccaaaa aaaatggcgg
     9961 caaggtcatt gtcaatgtcg gattgattgt tgaagaagga tatgatgaaa ttggcattgc
    10021 tgcggacgat gtggacgctg ttgtctactg gcgggacacc gagcagtcgg cgacgatcaa
    10081 acaccgtgcg aaatgggatt gtctgacgct taacagccgc aaaacggaaa aagagggtct
    10141 cgcccgcgtc aaagtcatta acgcttttct gaaagtgaca ccgcgccgta aagcagttga
    10201 ctctttactg gcacggctgg gcacctacat tttcgttcag aatgcgaaaa aaggcgatca
    10261 cgtcgacatc ggtgtggggc ttcctgagga agtatcccgg ctgctctatg aaaatggcgt
    10321 gcttaaagat ctgaccatga tcaacgagag cggtgttttt ggcggagtcg ccgcgccggg
    10381 cgttttcttc ggtgctgctg tcaatcccac ggagatcgtt tcttcagcgg aggccttctg
    10441 ccgtatttac aaacgtcttg actgggcgat actgggtgcg ctgcagattg attccagcgg
    10501 taatgtgaat gtgtccaagc gcggcgacgg tgccagaaat tatgtggggc cgggcgggtt
    10561 tattgatctg attaccaacg ccaaatctct ccttttctgc agtgcctggg gcgatcgcgc
    10621 tcagtttgag ctgacgcccg ataccatcaa agtaaccgac agcggaaaag ccaaatttgt
    10681 cgatgttgtg gacgagatca ctttcaacgg cagcgaagcg cttcgccatg gcaaaaaagt
    10741 gttttatatt acccatgtcg gtgcgtttca gcttacagac cgggggatgg aactgattta
    10801 catcatgccc ggtatcgatc ttcagaagga tatcctggac gctacttcga tgaagattgt
    10861 gcttcccgaa tccggagaac cggaattggt tcctggtttt gttatgacag gaaaaggatt
    10921 caacatgacg cttcctgatt gaggcggatg gaatccaagc cccctgctca ggtattctac
    10981 tcatcagccg gatatgccgg acagacgaca ggttgcatat tcggacgaag gacagacact
    11041 acggagatca gagaatgctg aaagataaaa tcgtacgggt gcggctccgc aagtttttca
    11101 atgagcagaa gcccacatcc tatgtgggga agtgcgttgt tttcactaat gactggatag
    11161 tggttgaagg cttcggtatc atgctgaccc gggcgagcaa aaatggcgcg cagatagacg
    11221 cgcgacccag ccagaccgcc atcccgagtt ccaatatcga gtcgatccgt gttctgcctg
    11281 atgacttcgc atacgggagg ctgcagctga gcacagaggg gcagcagctg gtgattgtag
    11341 tgcccaatgc acagccttgt gtgattggag aaatcggcga aggctgagtc tgtgcctact
    11401 catatcccag aacagcggcg agcctggcgg ggtagtccgt cagaatgcag tccactccgt
    11461 gcgattccag atgtttcatc tcatcttctc tgttcacagt aaaaaagcag aggagtatcc
    11521 cacgttcctg aaagcgccgc aattcttccc agctcagatc aaactgcttc tgcaattcca
    11581 acgacagaac aaacattctg tgcggcggtg tgtagctgtc ccaagctcca ttcctcattg
    11641 cggcaatggc ttcggcggcg tccagcacat cgtagcaggt ggcaatctca ggcgcctcag
    11701 atttgaactc ggcaagggtg accggattta cagtcgctac aaagcaccgt tgctctgcgc
    11761 acgcttctct gatggcggcg gcggtagctt ttgcaacttc cgctccgtcc ttcatttcga
    11821 tcagaaaagg gtgatccggt gcggcagcca gggcttcctg cagcgtggga atgacgacgc
    11881 cctgcccccg gtatggatac tcttttctgt cgcgcgtgaa ttgccagccc gcatccaggg
    11941 cgcgcaatgc ttcaaactgc atgagcatgg caacaccgct tccgttggtg acgctgtcca
    12001 catccagatc gtgaagaaga atcagttttc cgtcggccgt ggtctgaacg tccacttcta
    12061 taagggcctg cttccaccgg tctgaaaaag cccggatggc agtgactgta ttttccggcc
    12121 attccagtct gccgcttcga tgccctccca gacgaatgga aaaattgcct tctatgatac
    12181 tgtcaacggt ctgagaccag gaggagaggg gcacgagaaa aacggcggtt atgagcaaag
    12241 agattcgttg ggcagtcaca gaaaagctcc tttccggcac aatacagctc tgttctcaag
    12301 acctgaaagc gtaaaaagaa gtttcgtttt ccggcgtttt cggaaaatta ttctgcgctg
    12361 ctgcgctccg gtgctttgtg tatccagtaa atattccgga gatataccac tatcgtgctc
    12421 gctatgcccg tggcgtaaag ccattcgagc cgctgcaggt gcgagctcat gagaaggagg
    12481 gaagcaacga gactcaggta ccagaaagaa gcggggacaa tactcttgcg cttcagctcg
    12541 gtgaccgccc actgaatcag aaaacgaagg gcaaaaagac cctgacctgc cacacctaca
    12601 gcaatccaaa tccaattgcg tgcctgatcc accactggaa tatcccgggt gctttcatat
    12661 tcgttcagcc aggtccaggc gacgagtaac attcctgctg cgatcatcag cagaacagcc
    12721 ccctgaaaaa gcagggagcg gcgcggggtc agaagtcccc gttcacgcca gatgtgcacc
    12781 agattgcgtg cataaatcag gctgttaaag cagtgactca aagcacctac cggagagctc
    12841 agccatagcg ttccgtataa aagcagaaaa atggagccga tgccgctcat gtaccagaag
    12901 ccgatgggaa tgacactttt tctctgccgc tcagaaacga accactgcag gtaaaagcgt
    12961 ccgtaaaaca gcagagcacc gatatagccc agaacgggaa gcagcaaagc agacaagggg
    13021 aatcctttcg aaaaaaaaga gggaaatgga tattccattt cccccatcat accaattcgg
    13081 gagggtgacg tgaaagaagg cgggctaaat aaataaatta acgttggact gttccgccgt
    13141 tccgaggtaa gcaccgaccc cgcctatttc aatcccgtcg ataagttcgt ctttgtggat
    13201 gcccatcacg tccatgctca tggagcaggc gacgaggcga acaccggcag cgcgggcggc
    13261 agctaccagt tcttccggcg ccataacccg tttgtttgcc atcacatagc gcatcatttt
    13321 tgagcccatg cccgccatat gcattttgga aagtttcagc ttttccggac ctttaggcat
    13381 catcatgccg aacatgcggt cgaggaatcc ttttttcacc gacggcggtc tggatttctt
    13441 cagcacattc agaccccaga aggtgaagaa aatagtgact tcgctgttca tggcagcggc
    13501 gccgttggca attacaaaag tcgccatcac acgatccaga tcatcactga aacaaataat
    13561 agtcttggaa tttttgccac tggtggctgc tgatgcagaa cccgacgctg ccggggcagt
    13621 gcttgctgcc ggggcagtgc cgcctttgac cagccgggca agataacggt ttccgtccgg
    13681 gcgcgcttcc agcagcgtgt ttccggtgct cttgcaccag gccggaatgt cggcaatgaa
    13741 accgacatcc gtcgaggtca cttccagcgt gtcgccggga ttcatgccat ccatgagtcg
    13801 tttcacctgc gttataggac cggggcactg aagcccgcac acatccagct gggtcacttt
    13861 tccggcaggg gcgggtgatt tgcctggaat ggcaccgctg cagggggaac agttttgccc
    13921 ggcggcaggt gcagccccat ggctccacgc ccaggtgcgg tagccgccat tgatgttgat
    13981 cacattaaag ccgtagtttt ttaattgtct gtaggcgacg tatccgcgaa ggccgacggc
    14041 gcaatacaat gcaatggtac ggtcacgggg cagttcatta tggcgatttc tcaattcatc
    14101 cagcggaatg attaccgcac ccggaataat accgcatgcc gtttcatcag gcatacggac
    14161 atccagcaga aaatgttctt tggtcagatc gcggcagtca atctgctcca gatctttttt
    14221 caccacatta ttggcggtaa agccaagcat gttgatgccg ctttttgccg cgccgaactg
    14281 aggcgcatag gcaagttcgg aatgctccag atcggctgcc gtcataccgt ttgccaatgc
    14341 catggcaagg gtgtcaatcg ttcggcacac ttcttccggc ccgatcacct gcgcccccag
    14401 cagtttccct tcggggctga aaagcccctt aatgctcact ggcacggcat tgggatagta
    14461 ctgaacgtgc tgcataggat ggatataaac actgaaatac gggacgcttt catttttcag
    14521 tttcttttcg ctccagcctg tgcacgctgc ctgctgattg aacacctgaa caatggaagt
    14581 gcccagcacg cccggatagg cagcttcacg cccgcagata acgtcagcgg caatgcgccc
    14641 ttgtcgattg gcgggtcctg ccagaggaag cgttgccgga gcttgtgttg cccggtccca
    14701 cacctgtaca gcatcacctg cggcaaaaat ggaggggttg ctggtacgca tctgcgcatc
    14761 cacaacgata tgtccggaag cagccagctc aagcccggca tcccgtgcca gctcgctgcg
    14821 gggacgcaca ccggcagaca ggcagatcag gtcggcagga atattggttc catcactcag
    14881 cgtgactccg tgagcgtcaa ttttttccgc acggacaccc agcctcactt ccacaccgtt
    14941 cagacgcagt tcctgcagga gcggcgcagt catttcttta tccagcggcg gaagaatctg
    15001 atccagcatt tccaccagcg taacgttaag ccccagatgc ctgaggtttt ctgtcaattc
    15061 cagaccgatg aaaccggcgc cgataatgca ggcacgctgc gaaacggcag ccttcgcctt
    15121 gatcttatcc atatcgtcca ggctgttcaa cgtaagcaca tgttcgctgt cgattccggg
    15181 gatgggcggg cgaataggtt cggctccggg cgctagaacc agcgtatcat aggaacggcg
    15241 gtaggtttca cctgtttcca gattgcgcac ctccacctct ttttcttcag gcaggatttt
    15301 cgttacttca tgccgtacat taatttcaag attgtacctg ccccgaagcg tttcccggct
    15361 ttgaacaatc atgttggcgc ggtccgcaat aatgccgccc acataatagg gcatgccgca
    15421 gttagcgaaa gaaacagcgc ttcctttttc ataaacgatg atggttgcct cttcatccag
    15481 acgccggatt cgcgctgcac aactcatgcc gccggcaaca ccgcctacta tgacgataac
    15541 ttttgacatg ctcttaaaac tccttttcct gtaattgacg gaataatggg tttctgtatg
    15601 actgtggaaa acgtcaggtg ccgcctgtcc gtgtaatttc ctgcgtctaa ttcgcatacc
    15661 ggtttgcttc tatgcaagtg cgttttgcag gacaccggac aaagacagtt tttgacttct
    15721 gatgatgaaa acacttcctc ggacggcatt ttttatgaaa taacaccatt tgacaggaat
    15781 ttattcatcg gaacatgcgg ctggctaaaa gttttttagc tgatctttaa tggagtttac
    15841 gagagcgcct atatccgctt caagacccga cggaagcaga gcgctctgaa aaggataggc
    15901 gctgtaaaaa atgcgggtaa agttcaacca gaggcacacg cagatgatga ggccacccag
    15961 aagaaatcct gcgtatcgaa gcgcaagaca gctgaagatg ctgagctgca gtcttttctg
    16021 ctccacccag acctcctgca gttcatggtg gcgtgcatct ttcagaaaag cccgcagtgt
    16081 tgacagggga tagtaaatgc cgaagaaaag gagcagaccg acacaaagaa tttcgcccag
    16141 cagctgcatg ggatactccc acacatattc tctgttggtg ctgtacagag caggcatcag
    16201 cagcaacagc agaaaaagtg cggtgaagcc cgtgaaaagc agtttctttc ctgttccggt
    16261 taccggcaga aagggcaaat gcgtgcaggg tttcaggaaa cgacgcagca gaaggagagc
    16321 aaaatagaag agggtgaaaa gcacgacact ttcgagaata agcacaaaag gaatcagaag
    16381 acgtcgggca tacttttgtg cgagagcgtc accggaggca ttgaactgat ccagttcctt
    16441 cagcagattg aggagctttt gcgaccgttc cgcatactcc ggctcggcgg gaagcccgaa
    16501 gttgtccgca agcctcccgc gctgctgatg cactgccaac tggatctgga gagcgtgctc
    16561 caactggcgc agggcaggcg gcgagtctcc cttattcatc cccagaaggc gcatactcat
    16621 ggcttcgatc ttttcgaggt ggctgttcaa ttccgccgca ttttcctgag ttgcctccct
    16681 atccaatgca gcgcagaaaa gaagagtgga attatgaatg gtctcaaaaa tccgatcggc
    16741 tgtcatgtaa tatttatgca ggtagccttt tccgtggcgt ggcaggctgt catgccaaag
    16801 gggttcggga acaagcaggg gatcgccgga agcattggcc cgggcaatca gagcgagggc
    16861 attactgcgc tcctcttttc cttctgaaga ggcaaggagc gccgccgatt ccagataacg
    16921 cggcagtaag ttgcggggcg gattgatgcc cgcttcccgg aaccgctccc gcgcttcgcc
    16981 atactgcccc atctcgtaaa gatgacagcc gtaattgatt agcagcgatt cgcttttagg
    17041 attgctctga taggcgcgcg cataggcggg cagcagtttt tccgcctctt ccacttctgc
    17101 cagcgcttcg agataaagcg cagagggtgt gtctttttct ttgctttcac gctccacaac
    17161 ggttcgcaga ataggccgtg cctgtgcccg ctgcagagtc agcgccattc tgtattgcgt
    17221 ttcgctgtgg tcgtagcgca ggtaggattc tgaaaaccac caggccgcgg taaggacaag
    17281 aagaagggtt accgtcatgc ggaaagcgcg ctgtgaccgg cgaagtttcg tgtggtcaca
    17341 acgcggtatt ccctgcagcg gattcacgct cacgcttcac ctcccatgag gcggcgggca
    17401 atcttcttgc cttcctccac accgggctgg ttgaaggtgt ttacatggta gagccgtcct
    17461 gccatggctg tctccacttc aagcagatag agcacctgtg ccacggtata ggcattcagg
    17521 cggggcagga taatccgcac taccgggcga tgactcgcct tcagcgcgtc catggtgccc
    17581 cgcagttccg ctgccatcag tttgttcatg ctcttattgc gcaggtaatc caactcttta
    17641 atggatgaga gcgtgtcggg gatgcggagg cttgtattga aacggcgtac ttcaagaata
    17701 ttaaagatct tgtcgttcgg tccttctctg tagagctgca cctgcgaatg ctgatcggtg
    17761 gcacccagcg ccttgatcgg tgtttgccct gtgaaaacat cgttgccgtc cagatctttc
    17821 tgttttccca gactttccgc ccacagctgc cggtaccagt cggcgatgtc ccgaagcgca
    17881 tctgcgtagg gcatcatgac agacatggtc ttgcctttgt ttacgtccat cagatactga
    17941 atggccgcac gcagataagc cgggttttcc atcagcgacg ccctggagca aagggtatcc
    18001 atggcggcac agcccgccat catggcatca acatccatac ccatcatggc agcagggaac
    18061 atgcccaccg gcgagaaaac ggaaaagcgt ccgcccacat tgagcgggat ggaaaaggag
    18121 gtgagcttgt agtttttggc aacggggtga agcaggcttt tgggcgcgcc ttttccggtg
    18181 ggactggtcg tgatgaccag atgttttttc attgcctttg cgccgaccgc tttttcaaga
    18241 agcgctacaa cgatcatcag ctgggacatg gtttccgccg tttctcccga tttggagatc
    18301 acattgaaca gtgtcttttt gggagggcac agccgcagca tggcacggaa ggtgtccgga
    18361 tcgatattat ccatgacaaa aaggcgcgga gctcctttgc gccgcttttt gctcagtgtg
    18421 ttgaagtaag gcggattcag ggcggtctgc agtgcggtga tgccgagggc ggagccgccg
    18481 attcccagta ctaccaggtt ctcgtatccg aaactgctga aatgcgcagc agcctttttt
    18541 acatccgcca gcgtttcttt gtctttgtac agatcataaa atccgtattt cccttccttg
    18601 cgttcctgct gcaggatttt gtgcgcagcc agcacgcgct cttcgatagc cgcaaattcg
    18661 gcaggcgtga taccgtgtgc cgcacttaca gcactggact ttgcacggga aatatttatt
    18721 tcaatgtttg catatacggg tgcctgaatc tctttcatgg gcgcaatcct tgtacctctg
    18781 tctgggggtt gtggtgcttg tcagtcggtg aacatatagg cggcatcaac cgggctgcat
    18841 tttatgcatg aaaacaaaac atgcacaaaa aagacatctt ttccgtgtca tgcccggatg
    18901 atacaacacg ctattgtagc atattcaggt ttttcagaat cgctctcagt tgtcaatgaa
    18961 accgtgatac agccccatat aatagggcag cagaaagacg gtaccgcttc ccagcgtatg
    19021 gccttttccg ccgtagttca gattccaggg atcatgattc cagtggttga aatagcgttc
    19081 ggaaacaggc agaacttttc cgttgaccct gtgtccgcgc tggggcactt ccgattcata
    19141 aggttccgtt gcctgctggc ggggcagcag cacgatatcc aggcgatggc tgttatggtg
    19201 atcccaggcg acccgatcca gcggaaaatc tttcagcgtt tcaacagaat catcaagcca
    19261 gccttcccag gggctgatgt cgtaggtgtc ccagggattg gtgtgctttg cattccgccc
    19321 gaagactgca tacataaagt tgaaaagggg attcatttcc ggccattcat tgatccaggc
    19381 ggagaaaaat gagtacagaa tttcatgttt aagcgcttta tcctgactgt atttcaggag
    19441 attgtagtag cacataaacg ccatttcatc gtctgaatga ttgcctgatc cggggccgaa
    19501 atggatttta tagatcatgg cgtttgttct gaaatggtgc ttttcaatca gttcattggc
    19561 aatcttcccg tatttgggat cgcctgtgac atgttctgta gcggtcagat aggaaagcat
    19621 actcagcgat ttaaggccac gctcttccca ccacagcgga tctttattca gcgaatccgg
    19681 attgtagata gaccagcgtg ttggtgtgcc gtcaaaatct accagattga aatcgtggtc
    19741 gataagatga tccatcagat cacggatgac ctgctgtacc gcttcttttt cttctttcgt
    19801 ttcggcaaca aggtcataat aggcagggta gaagaagaaa tgcccgtcca gttcgtccga
    19861 gcttgtgtcg ctcttccagt accatttgcc gtcaccgctt ttcggccagc gtggctcata
    19921 gactttccag agcacgtcat ggttgtcccg gtgataggtg tcccgttcta tcctgccttc
    19981 attgggatcc ttcccgtcgg ttggaagaat tgtgcgggca acatagcctt tgggggggcg
    20041 atgatcgcct gtctggggaa cggtctgcag aaaacgaagc gcttcaaagg ctttgtcagc
    20101 acgttttttg gcttcaggat cacgggtagc gccataagca aagcattctc cggcgccgta
    20161 catacttgtc cacaagccgt cattgtcgct gtccgtatac acgaccgtgg agcggtcgcc
    20221 tgctttttcc agcttcactt ccgaagtata gccgaagggc gtgcgcttga tgtatttatc
    20281 caattcctct tcgtaaaagg ctgctttttc cgccagcgtc atgggcgtga agccgatcca
    20341 gccgatgccg tgctgtgttg ccgcccaggc attccctgcc tgatccacca gcagatcatt
    20401 gacgtgatca tccggcagcc agcgccgtcc ctggcgataa tgccaggtcc cgtttttaaa
    20461 acggacgatt cctttggtag tgccgaacca gacagcgcca tcaggaccgg cggctatgct
    20521 cgtaaagtca ttgtagggaa gcccttcgtg ccctgtgtag aatttccagc cgtccgctgt
    20581 acggcaggcg agccctgcaa gcatggcaac ccacagattt ccgtcgctgt ccaacgtgac
    20641 accgcggaca tcgcaggcac cccagagcct gccctgtccg tcacaggcag ggcttttttc
    20701 atatacaccc tgatcatttc tcaggaaaag accttctgcc gcggcaatgg caacaggagc
    20761 tttggcgtct ccggcgaagg accggacaat cacagcgcct gatcccgggg cagcttctgc
    20821 cggtgccagc gtataggcat ctccatcggg aacgagcaca aagcgctgag gcgtgaaatc
    20881 ccggaccggc ttccatgttg ccccttccag tcggcaggca cctgcggcag tcacagccac
    20941 aatacccgtt tcgtcctggt gcaaagaaag gactttctga tcgggtaatc cgtctgccac
    21001 actgtaattc ttcgctattt cctgagggaa tacgcctact tttgccggaa cctcgtcggc
    21061 tgcaaaaact gccgtgcagc aaagcagtgc aacaagcaat ccagtaacac aattacgcat
    21121 gatttctcct ttagacagtt tcagggtttt cgccggataa aatacaaata caaaaaaaca
    21181 ccctctgcac ctcgtctatt tgtaccatac ccggatgatg gaaaggaaat aaagggaagg
    21241 cagaattcat gccggcacct ccggcaacaa ttaaacagag tgagtcagtg tggttactga
    21301 gggcgggcgt tgcgtgccag gatccgttct gcgccggact gcagtgcggc ggccgctttt
    21361 tccgctgtgg tgacgcctgc cataatctca gacaccgccc gtgtggcaag atcgcgtact
    21421 tcctgccatc ccggcgcatt gggttctgtc cgcgcaaagg caaggcagtc atagggcacc
    21481 ttgttgcatt cccatttctc ccagtgctgc tgcagctcag gctggtgcag cgtgctccgg
    21541 cgtacgggca ggtagcctgt ttccagagac cagcgtaccg atacttccgg cgaggtgaga
    21601 aatttcatga aagcccaggc ggcctgtatg tgcgcttcgg tggtattgaa aatgcagaca
    21661 ttgggcccgt acagcgtggt gcccggtttt gcaggatatt tttgcggaat gggcgcaata
    21721 ccccagcgtt ccgtatctcc ttccatgagc agcttcatat cgctgaagct ggaactggat
    21781 cgcatgacga aagcaatttc gtcttttgaa agcgccacat tatcttcgta tgaagagggt
    21841 gagaccagcc aggccaggtt ttcttttgcc aatatctcat agatccggaa gacggcaagt
    21901 gcttccgggc tgtcatacaa gcatttaccg tctttcagca catcgccgcc ctgactgaaa
    21961 atcattccgt tgacagtgga acagtcggca tggacaccat gtgcatattt tccggttttt
    22021 gcttttatct gtctgcactg attaataaaa tcgtcccagg tggcgggggg ggctgtaatc
    22081 ccggcatcct gcattacttt tttgttgtaa taaagaatga gcacactttt agcaaacgga
    22141 aaggcgtaca ggtgattatc gtggttggcg aagcggttgt tttccagtgc gccagggatg
    22201 taatcagcga gagcttcagc ggagagcccc gcttcttcat ccgccagcag aggctcgaga
    22261 tcaacggcag cgcctgcatt gatgtagtcg ttggtcatgc tttcataggc aacagccatg
    22321 gcggggagta cgcctgcgcg gatactggcg gagacttttc tgaaaatatc cgtatagttg
    22381 cctgcccgtt ccactttaat ggggataccc tgccagctcc ggttaaattc tgcggcaagg
    22441 tcctgcagca gctgggcact ttccgtggtc tgccggtccc agaaaaccgc aaaatccgga
    22501 tccagcggct tgaaggatct gtctttgctg cctccggggg tggcaggcgc gcaccctgaa
    22561 aaaagaggaa caagaagaag cgtgctgatc agtgaagcag tcacagctat aagcaatgcc
    22621 tttttcatga ttatccttta atgccggtac ccatggttgt ctggacaaaa taccgctgtg
    22681 ccacgaaata gatcagcacc gtgggcagaa tgatgatgga ggaggcgcac atcagaagat
    22741 tgagccgtgc tccggcatca ctgtaaaaag caacaagccc gatctggaca acgcggcatc
    22801 tgtcatcggc ggtaacgatg agcggccaga gcagagcgtt gtaactgccc agaaaggcaa
    22861 aaagcgcaac agtcaccagc gagggcatga ccagcggaat cccgatcctg acgagaaaac
    22921 gaagatggcc gcatccgtcc agaaccgccg catcgtagaa gtcgcggggc agcgcaagaa
    22981 aagcctggcg catcagaaaa atagagaagg cgctgacgca ccagggcaca atcagcgctg
    23041 cataactgtt gtaccagccg agccgggaaa tcagcacgaa attggggatg aaggtcagtt
    23101 caaaaggaac catcatggat cccagaataa tgaagaaaag aagctgtcgc cctttaaact
    23161 gcaggcgggc gaaggcgtaa cctgccataa gggaagtggc gcagacggac aggcataccg
    23221 atcctgccac aagaaaagaa ttgaagaagt agaggccgaa aggggcggca cggaaggttt
    23281 ccgtcacatt tgaccattgc catactttcg gcagaaggtt caggctgggt gtgcttgctt
    23341 cattcagggt tttaaacgaa gcacccagca tccagagaaa aggatacagg gcaagaaaag
    23401 cgccgcagca gagaaacaga tataaaagca ggcggttgaa tttcatgagt agaaaacctt
    23461 ccgcccgagt acccgccact ggagcagggt taatgccaca ataaccagca cgagcagtgt
    23521 ggcgacggca gcgccgtatc cccagtagcc gtattgatag aactgggcat atacataaag
    23581 gataagattc tgcgtgtcag gcagtcctcc cgcatgagtc aatgcgtaaa agctgttgaa
    23641 tgcctgaaag gccttgatca cgcccacgat ggacagaaaa aagatggtgg gtgagaggag
    23701 cggcagggta acatgccaca tgagccgcca ttctccggca ccatccattc gtgccgcttc
    23761 ttccagttct cggggtatga cactcagtcc cgcgagaaag acagcaatca tgaatccgct
    23821 tccgtgccag atatcaaaga gcataataca gcagagcccg agactgggtc ccaggtctgc
    23881 agggatccag ccatgactca gcagatgcag gatcccccgg ggctccagaa gccagttctg
    23941 cggggaaaac cccagatagc tgaaaagaag attaaccaca ccctgctgcg gatggaagag
    24001 actccgccag accatggcag cagcaacagc ggaggtgaca taaggcagaa aatagagaga
    24061 gcgcagaagc ccccggccgc gggtaatttt aaaaagaccg aaggcaataa taaagctcag
    24121 agatacagta agaggcacca caccgagaac atagtaaagc gttacaagaa agctgcgccg
    24181 gaaatctggg ttattcaatg cctccgtgta gtttgccagc ccgacaaaat caccttctcc
    24241 gtggcgacct ccgaaaaaac tcatctgcaa cgaggcaccc atggggtaaa gagagaaaag
    24301 ggaaaggagg atcacagcgg gcaggagcag cagcagagcg gcaggccagt cgcccagccc
    24361 gcgcctccgc agtgcgtcca gtccgtcatc aagcataccg aggcagcggt cacctgccac
    24421 tgcaagcagt acgaagggga aaagaaatat ctgcagtcgc tttttaaact gcgtcataag
    24481 tcattctcca gggttgaccc tggcaggcgt ttctgccttc aaaaggcata gagtctgcag
    24541 tgcgtcagca ggaagcggcg tgatgtcgcg ccaaagcaca tccggcactg cccagcactc
    24601 ctgaatcatt gccgagcccg gcaggcagga tttgacagcg ggcggcgggt acttcaaagg
    24661 cattttcctt caccgttttt ctgaccgttt catagagcag atcgccggat ttgctcatgg
    24721 cgccgcaaag gataatcctt tccgggttca gtgcattgac catggaggcg gcgcctagac
    24781 cgatccatac agctgtttcc gtgaatacat cacgggcaaa ggcatcgccg gacatcgccg
    24841 cttcataaat catttttccc gtcaatttgc ccgggtcatt atcgcacaag gctttaagcg
    24901 aggtgtccgc tccgtcccag ccttcttttg cggtacgaag cataccggta acagaggcat
    24961 aggtttccag acagcctttt gatccgcatc cgcacagcct gccgtctttc tggactttaa
    25021 gatgtcccag ttccgccgct gtgccgtcta tgccgcgcag cagttcaccg aaaaccacaa
    25081 ttccgccgcc cagacccgtg ccgagcgtga agacgacaat actttccgcg ccctgaccgg
    25141 cgcccatcca gtattcgcca taacaggcta cattggcgtc attgtccaca tagcagggaa
    25201 cgccgaggcg cgctttcatt tcgtccgcca gaggcacgtc cttccaaccg ggcagattgg
    25261 gcggagagaa gacaacgcct gaacgccagt tcatggggcc cggcgcaccg aacccggctg
    25321 caagcacatc ggccatggtc agcccggcag aagcaatcag atctttgaca aagcctgcca
    25381 tcagatccat gacgggaccg gggcctgcgg cggcgcctgt cgggcagtcg ccctgtacaa
    25441 tgattttttt gtcttcagag acaacagccg ctttgatatt ggttccgccg agatcaacgc
    25501 caatgactac cttactcatg ctgttccttc tcctgatgtc ttgatatagt tcagggcagc
    25561 ttccatccgt tgcacggatt cctcacgccc gagcagtttt acaagctcgt acaatcccgg
    25621 cccgacggtt tttccggtga gcgccagccg ggtcccgttg accagcgcac cgattttgag
    25681 ttctttctgt tccgcagcgg cctcatagct ttctttaaga gcggttaccg tccactcgtc
    25741 cgcctgccgc atgcattcca gagcggcttc cagcgcctga agtgacgtgt cctggcggaa
    25801 aaacttccgg tctccttttt catcatacgc agtcggagca gcaaagaaaa agtcggtgta
    25861 cgggataata tgattcagtg tcgggatctt ttcctggcag atagccgcca tttccacaag
    25921 ccaatcccgg gagcgttcat ccaccggaaa gccctgttcc cgcaggatag gcagtacacg
    25981 gtcgcacagg gcatcagggc tcagcatgcg gatatgctgc ccgttcagcc aatcgagctt
    26041 tttgcggtca aaacgcgccg gggatttcgt cagtcttttc agtgtgaaaa gctgcttcaa
    26101 ttcatcgagc gtataaaatt cgcgcccttc ttcttctgat gtccagccga gaagcgaaat
    26161 ataattgatc attgtttccg gcaggtaccc gtctttccgc cagtccagca catttgcgcc
    26221 atgaagacgt ttactgtatt ttcgcccctg ctcgtcatgg atcagggggt gatgtgcaaa
    26281 tttaggcagg ggatagccca gtgcgttaaa aagcatcaca tgcctgccgg cattggtcag
    26341 atggtcatcg ccccggatca catgggtgat tttcatgaga ccgtcatcga cgacgacggc
    26401 aagatggaaa atggcatctc cattggattt gacaatgata aagtcatcgt attctttgtt
    26461 ctggatccgg acgggcccct gaataatgtc gtcgataaca gtttcccctt cgggcacgcg
    26521 gaaccggatg gcataaggcg tatcgccttt cgccgcaatt tcttcgggcg tgagatttcg
    26581 gcatttaccg gaatagcggg gcggccgttt ttccagtgcc gcctgttccc gctgtgcgtc
    26641 cagttcttct ttcgtgcagt aacagcggta agctttgcct tctgcgacca gtcgtgccgc
    26701 ttcttcgctg tagagctgca gccgctgtga ctggcggtaa gggccgaagg cgcctttttc
    26761 cggttcatcc ccgaattccg ggccttcatc ccattcaata ccaagccact tgaatccttc
    26821 gcacatggcc tggacatatt gttcttccgt ccgttccgca tcggtatctt ccagacggag
    26881 gatgaaggtt ccgccgttgc ttcgggcgaa aagccagttg tacaacgcgg ttttggcggt
    26941 accgatgtgc agaaagccgg aaggagacgg ggctacacga caacgtacgg cggaagtagt
    27001 cataacagtc ctttttttta ctacagggaa ggggttcggg gttatggagc atattttaga
    27061 gcaatgcccc gcactaatca accggacgct ttttctccgg tttcacggcc gtgacaggaa
    27121 aaagcgccgg cacatccgct caggcgtgcc gacgctccgt cattcgtcat aaatctcgtg
    27181 atcagggata aatcgttttt tcgtagtggc gcagattttc gccgatataa acctggcggg
    27241 ggcgctgaat ccgaagatcg ggatccttgc tcatttccag ccagtgggcg atccagccgg
    27301 gcatgcgtcc gatggcgaag agtactgtga acatttctgt cggaatgccc agggcacggt
    27361 agaggatgcc gctgtagaag tccacgttgg gataaagttt gcgctcgata aagaaatcat
    27421 ccttcaacgc cacctcttcc agatttttgg cgatatccag cagcggatcc tgaatgccca
    27481 tttctgtaag cacgtcatca acggcttttt tcagaatttt cgagcgggga tcaaagtttt
    27541 tatagacacg gtgcccgaaa cccatgagct tgaagggatc atctttccgc tttgccagtt
    27601 ccacatattt tttatagtcg ctgccgtcgt cggcgatacg ctgaagcata ttaagcaccg
    27661 cttcattggc accgccgtgg agccgtcccc acagcgcatt gatgcctgcc gatacggaag
    27721 cataaatatt ggacatggag ctggcgacca tacgcactgt ggaggtgctg cagttctgct
    27781 cgtggtccat atgcagaatc agaagcatat ccagcgcatt ttccatgact ttgggcacca
    27841 catattcttc ggcagggctg gcaaacatca tgcgcaggaa gttggcggaa tagctgtgat
    27901 ccgtgcgcgg ataaatatag gggagcccga ccgatttacg gtaggagaat gccgcgatgg
    27961 tctttacctg accgatgagc cgctgaatat gctggtccat attgtcgttt tcgccgccta
    28021 tatgcgggta gaacgaggag atggaagaca ccatggcact cagaatgttc atgggatgtg
    28081 cgtgagcgct gtagttatcg aaaaaattca cctggctcgc atgaatcagg ctgttcagcg
    28141 tcagctgctt gcgccattcc atgaactcgg cttcagacgg catccgcccg tagatcatca
    28201 ggtaggcagc ggcggaaaag cggacggagc ccgccagttc ttcaatggga taaccccgat
    28261 agcgcaggat cccccggtca ccgtcgataa aagtaatggc actttggcag gagccggtgt
    28321 tggcataacc cgggtcatag gtgatcagcc cggtggtatc acgaagttta cggatatcaa
    28381 gagcaccttc gttttccgtg cccacgatga cgggaatatc atattcctga ccgttataaa
    28441 tgagttttgc cgttgtcatg gttatactcc tctagatttt tcccggtgga tgatgcgcag
    28501 gaaggtttcc cgggtgagat tcgttccttt accttcgccg cctgatggag aacatcctgc
    28561 ttgtgcccaa aagcaatact tttttcgttg taaaagcata aaaaccggaa aagcaggaaa
    28621 tccgaatggt ttcagcccga tgccctgtcc tgatacaggg gtgtcccgac tcctgctcgg
    28681 aaggctggag tatagcaaaa atggacatat tttgcaattt aaagagcaga cctaacgctg
    28741 gcggaggcct atatgttctt gtttttggtt tggcacggtc cggaggagta caatacggca
    28801 atagaaaacg gatttttccc acctgttaca tcccggttct tttctgacag gaaccgcttt
    28861 acatggagaa ctgcctgatg aaaaaagtat tactaggtat tctgactgtg cttctgatct
    28921 ttgttctgct aatcggtctc ttccttttca gtcgtacgcg cggcccgcac cgcagttatg
    28981 aactggactt tgtcaagatt ggatcggctg ctgatgccgg agcactgctg gtgggtgttt
    29041 ccaaggtcga tatcacaccc gacctgtctc tctacgatac ctactatgat gcggataaca
    29101 acaataaatt caatccggca aaaaaggaca tgaaccgtct tgaacgcctt gcactgcgtt
    29161 tcggtctgat caaggaaggc caggacacct ggaccgacag caataaaaac ggacgatttg
    29221 accctgtatg gatggcgggc ttcagtccca atcgtcccgc caaaggcgtg catgacccta
    29281 tctgggcgcg tgctatcgct ttccggaaca acggcgttac cgttgccatg gtcaccctgg
    29341 atgccatcgg catttttcat gacaaaatca tagatatccg taaaatgatc gatccttccc
    29401 ttaatgtgga tcatgtgatt gtcacctcca tgcacaacca tgagacgcct gatacgatgg
    29461 gtaactggag cggtcttgtt cctcgccccg ccaactttga tcagcgtcat atgaacaatg
    29521 tcaaaatggc atgcaagaca gcagtggaac aggctgtccg cagcatggtt cccgcggaca
    29581 tgtatctgaa aaccattatg atcgagcccg aaggtctggt caatgactcg cgtaaaccca
    29641 ttgtgatcga cagacagatc tgcacagcgc gcttcaaccg tgccggcaca gacaccaccc
    29701 tagccacact ggtcagctgg ggcaaccacc ccgaggcggc gggaagtgac aacccgctga
    29761 ttacctccga cttcagcggt tactggcgcg acggcgtgga aaaaggtgtg tctgacccca
    29821 acggtgaaga aggaatcggc ggcatgtgcc tctattttca gggcatggtc ggcgggctga
    29881 tgactcagct tcacactacg gtaccgcatc gcaacggcgt agacatgatc cgggaaaatg
    29941 gctttgcgaa aacagaggca ttgggcgaca atctggcgct gatcacattg cgcggtctga
    30001 aagagccgga tgtggtcaag agcgaagatg tccgtgttgg cgttgccgcc aagacaattt
    30061 atgtgcccat ggcgggtctt ttcaaatatg caatcatgct gggactggtt cacccgggca
    30121 cctactggaa agacggagcg attacctcca aaacggaagt gaatgtggtt cgcgtcggcg
    30181 atctggaaat catcactgtt cccggcgaac tttatcctga aattgccgag ggcggcgtgg
    30241 aagccaagcc gggtcgtgac tttgatattc cgccgctgga agtgccgcct ctgcgcaagg
    30301 aaatgcgcgg gaaagtgaat atggtttttg gtcttgccaa tgatgaaatc ggctacattg
    30361 tgccgaagag ccagtgggat gtggaaccgc cctttgtcta tgaaaaagca cagtatggcg
    30421 aagagaactc cggcggtccg gatgttgctc ctaccattca ccgggaaagc ctcgctctga
    30481 tgaggcgttt tgctgaagcc tgggacaact gagtcagccc cgctgccgaa ttctgaaaaa
    30541 tatacgcccc gactgcttcc tgagtcgggg cgtattgctt tatcgagtac caggagtgtc
    30601 cggcatacgg cttttattcg gagggtacga tcgggctggc tttttcgctt tcgttggctt
    30661 cgcccgcatc aagcggcggc agatctttca cgctctgttc tgtgccggta ttgcctgcgg
    30721 gcacaaaacg cccgccctcg ccgacaacat ccaccacccg gtcttcggcg acaatggtag
    30781 tgccttcggg cagaaaaata cggggcgttt cgtgatcctg agcgtttaat gtccgtatct
    30841 cttttacatc gatatacagc tccgattcct ggtcattcac aatatggatc cgatagggaa
    30901 agtaatcgga ttcgcgcagg tagagattca cttcgaggaa gtactctgct tcctcagatc
    30961 ctgtgcgggg gcggatccgg ataccctgtc tgccccgggg gtcatccgag accagcagtg
    31021 taagatcata ggcggcgcgc agggcttctg tatcgttgtc aaagcccaga aaaaagatgt
    31081 tggcgcgcgg gtggtcttcc acatagaaca tggtcatctg cctgatgtcg gcatcatatt
    31141 catacccctg ccgtccatcc ataagcagtg catgttcggg atcctccgtc tgaaaaagga
    31201 tgcgccgggg ctttgaatat cggagcacac catgggtaat cagcttttct tcaggcagta
    31261 tggtggtctg ttcaaattta gcttccagtg attcgatgcc gtcgcgcttc tgtttgaaaa
    31321 cactgaaaaa gtggctgata tcatcttctg cctgaccgca gggcatcagg ataagcgggc
    31381 agagcgccag aaacagcact gtcatggttt tagagatcaa tgtacgcatt atcctcttcc
    31441 cggctgtttt ccccgtcctg accagcagca acagaggaac ctgccgaagt ctttgtttta
    31501 ttttttcccg ttttgctttt ttctgcagcg gtgttttttt cggaagatgc tgcctgttct
    31561 ttcgtctctg cccgggggat tgtttcgatg agtgcctcat gcgccttcag aattgcgctg
    31621 agatcctggc gaagctgatc ccgggctgcg gtaagccgct gaatttcgcc ctgaagggca
    31681 ggggtgagct gctccatttt aaagacggca cgctctttgg caatacgtgc ttcttccacc
    31741 agtgcatctg cctgcagttt tgccgacgca atcatggatt cgcccatttt ctgggagctg
    31801 agcagggaag cgcgcaagga tgattccatt tcgcggtact tgtgaagttc ctgctccagc
    31861 gcagccgctg ttgctgcaag cccttccttc tccaggcgca gtgattcaat agtggcagcc
    31921 gcttcttcca gcagcatatc cacctgtact ttggaataac ccattggccg tgtgctgatc
    31981 tgtgccgaat acagctgttc cgccgtcagc agcggtgcat gccccggttg tacttcctga
    32041 aattctcttt cttccgtcat gatcaattcc tcctgataat ccgcctgtgc ctgcccgtca
    32101 gtgtgcgccc agttcgcggg atcgcttttc tgccgcgtca aaggcttcct gcacgagacc
    32161 ttccaaatcc cgcccacgca tgcgttccag agcggcaaag gtagtgccgc ctttggatgt
    32221 gaccttttcc cggagcgttg ccgggctttc acccgattca atcagcagtt tgccggcgcc
    32281 atacaacgtt tgagccgcaa gatgtgccgc tgtgctttcc gggatgcccc ggcgcactgc
    32341 cgcattaatc agacattccg tgaggtaaaa aaaatacgca ggaccgctgc cgctcagtgc
    32401 ggtgacacca tcgagccacg cttccggtac aacttctgca atgccgaccg attcaaatac
    32461 ggcgcgccct cgggcgatat cctccttcgt gcagttggtt ccggcagcca cagcggcggc
    32521 tcctgctcct gccagagcgg gcgtgttggg catgacccgg aggatgcgtg cttcggcgcc
    32581 gaaaagaacc tgcagccggg cgatggaaat acctgctata accgaaagta cgcatgcatc
    32641 gggacgggcg gctgcgcaca caggggccag agcctcttcc agcatctgcg gtttcactgc
    32701 cagaagaagg gtgtcacagt gctgcgcaag catttcagcg gaagcgacgc accggaagcc
    32761 cagcgcactg gcagcccgca ctttttccgt atcgggatca taaacagaaa tctgccgcgg
    32821 cgtgacggtt ccctgtgcga ccattcctgc cgcaatggca gcacccatat tgccgtatcc
    32881 gagaaagccg aagtcaccgg aaagtgttgc catacgctgt cctgcatcct gtgtctctta
    32941 ttgcgatgag ggggcgtcgc ctgcttcagt tccggggata acccggacaa ttctgccctt
    33001 gaactcatgg agcacagcgg cgattttctc atcctgttcc agattgtcgg tagacgtctt
    33061 cattgtctcc gccggttttt ttttccggct tatacgggga actgtcggcg ccgctagggt
    33121 aaaccgcacc gaacggatgt tgttagtgcg cccctgcaga aaagcgtgca agcttttccg
    33181 gttttcctcg tcttcgaaaa aggcgtgcgc ctgaacggct ccgggctcca gctcaagttc
    33241 cagctcaccc gtgtcttcca gaatcgccaa aggatgggcc cgtcccagcc agagtcccag
    33301 gctcagagat gtttttcctg catccagcac caactggcgc cactgtgaat ccaatgtgtc
    33361 ggcacccagc gtgatgcgct cttccttctg ccgaatcgtc gatgtgcctg aaggcgaccc
    33421 ggcttcagga gggttttccg ggggccgggg cattttcctt tctgtcgggg cggagggctt
    33481 ttcagcgggc gctggcactg gagcagtttc ttcggcaggg cgggagtcag ggatttctcg
    33541 tctgcccggt tccggaggaa gaatgcctcc cgcaccgaga tgcaccaatt tttccataac
    33601 cgtatcgaga gagacctcca catccacccg ggtgctgcgg atgagcatgg cttccagagc
    33661 ggtacgctgc gccagctgtg aatcaaaatt gttgcaaaga tcagaaaatg cttcgatgag
    33721 acggatcagt ttcacaacag gaaagcgtgc tgaccgtatc cgtattgctt ccatttccgc
    33781 ttcgggcagg cgcagcaagg cttcccgcgc cccggctgtg cggtagatga gcagattacg
    33841 gtaatagcgg agcaaatcct ccacaaactg ggagagatcc tttcccgttg cggccacgtc
    33901 atcgacaatt tccagctgcg ttgctatatc accctgcagc atggcgtcgc aaagatcgtg
    33961 aaaggctttc cagggaacca gcccgagcac gtcgaaaaca tcttcgaaag tgatggatgt
    34021 ctcgctgtag gtgatcagtt tgtcgagaat gctttccgca tcacgtacgc ccccttccgc
    34081 aacacgggca atagcataaa gcgcatcatc cgaggccgcc agtccttctt tttccacaat
    34141 agtgcgcagc agtgccacca gattgtggat ggttacccgg cggaaatcat agcgctgaca
    34201 gcgggaaata acggtggcag ggattttatg tgcctccgtc gtcgccagca caaagatcgc
    34261 atggggcggt ggctcttcca atgttttcag aagggcattg aaggcgggca acgagagctg
    34321 gtgcacttcg tcgataatgt aaatcttgta gcgtgaactg ctgggaacca tgcggatggt
    34381 ttcacgaatg tcccgcacgt catccacact gttgttggag gctccgtcta tttcgatgac
    34441 atcaatattt gttccctgcg tgattgacag gcagttgcca caggtgccgc aggggttcat
    34501 agtgggcttg tcactggatt cgcagttcag cgctttagcc agaatgcggg cagttgtcgt
    34561 cttcccgata ccccgcgacc cgatgaacag aaaagcatgg tggatgcgcc cggactgaag
    34621 cgcatttttc agtgtccgcg tgatatgctc ctgacccact acttcatcaa aagtctgggg
    34681 acgccacttc cgggctatgg cgacatagga tgaactggac acaggcactc ctgttttgcc
    34741 ggtagaggca cataaatgta tcagacaagg aaaaagaaaa agagcccgcg cacccacctt
    34801 cgaacaggta cccagaacgg cctcggaggc cgacggctca ggccaggcaa ccctgcagca
    34861 cacgagactg accggctgcg gctgcttcct ttcggacctg accgggttca ccagcgcccc
    34921 gttgagcagg acccagcctt caacaccacg taccccgaac ctgactaaaa ggcactgtgc
    34981 cctccagtgg gaattcaacc tcgctaaagc ggattgcgag ttacaggaca ccgctaactt
    35041 cccgtctagc gcaggcaaaa tggatcttat ccaactgatt tcaacggtag aagcgtatca
    35101 cagcaggcga taaagtgtca acttgtgcct ttattacctt tgctgtgttg tacaaaaaaa
    35161 cagtataccg cacaaatatt tgtcgttaaa tatggcgcta atcttccttt tcctgtaatt
    35221 taggttgaaa tacagagaaa aacgataaat gcagtcgtgg catgaaaaat gctctcttct
    35281 ctagtaaaga ggcagccgga caatgcttct cttcacgaca gcgtgttgtt gtttgtgctc
    35341 tctggcatca aattccggag ctttagtcgc catggttcaa aagaaaaacc ggaaccgaga
    35401 cggtttcaca ctccttgagc tgatgatttc tgtggcgatt tttgctatca tcctgagtat
    35461 tgctgttccc aatctgctgc gttcccggct ccagtcaaac gaagttgcga ccattcagaa
    35521 tctgcgctcc atcaactcgg cacagggtgc ttacaattca gccaaaaaag tctacgccac
    35581 cacctttgaa gacctctgta atgtagccct gccttttctg ccgtcgacct tcctgacaca
    35641 gcctatggca ggttatacca tcacgcttgc cggcgaagag gatctttaca ctgttgtcgc
    35701 tgagccctcc gagtggggcg tcaccggtaa tcgtggcttc tacacagacg ccagcggcat
    35761 cattcgcttt gccataagcg agcctgccga tgaaggcagc ccgccactct aaaccgccgc
    35821 acttttcctg tcttctgatg atgaaacgga cgcatcccgg ataatctggc ttttttttgc
    35881 gtgcccttcc caagaccggc acgctcccct tttccggaca aaaagaaaac cccggcgccc
    35941 atcagcgttt cagccaatga ttgcaggggt gaaatgaact cttgtatggt accggggcag
    36001 ggggtcgaac cctggacctc tggatccaca atccagcgct ctaaccatct gagctacccc
    36061 ggcacctgaa cctcgcagaa agcattatag ccttttacgc ccggataagt caattttgcc
    36121 ggtgcacgcc tctgcgcata tgcgtgtctg cagcattaca aacgggcgtc gctgcaaact
    36181 taggacaggc gtttattggg gagcacggga acgaggtgca caaaggccta agaaaaagtt
    36241 cttcccagcc aaatagttcg cggcgaattc gtgtattcgt ggcacaaaaa aagcggcgcc
    36301 agcaccgggg tactgacgcc gcaaagggtc aacagagaaa ttcaggat
//
LOCUS       DAOPWB010000013        33359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1248_length_33359_cov_5.577078, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000013 DAOPWB010000000
VERSION     DAOPWB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 33359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33359
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1248_length_33359_cov_5.577078"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKN92_02470"
     CDS             complement(<1..488)
                     /locus_tag="PKN92_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47688.1"
                     /translation="MSEKKKCGRFRLCLKWGFRIFLLIVLILAGILGYFFREGIYKRY
                     FLFPKQAKAWAQFKAERVEPGIATGWHEYRGMLHSHSELSHDSAAAFPEIVAALKKID
                     VDFICMTDHYEKGLADYSLGWNGMHDGILFIRGYELDHGLMPWGIPEGTVFAASDEPR
                     ALA"
     gene            complement(843..2852)
                     /locus_tag="PKN92_02475"
     CDS             complement(843..2852)
                     /locus_tag="PKN92_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNZ47689.1"
                     /translation="MKQNSAGTSGISRRTFLKQSGCTLAGAALGRSFHIGKARALRQG
                     KVIMLGYDGMESSIVSAMMERGDLPHFARLKALGGDYTLTTTIPPQSPVAWSSFATCK
                     NPGAHNIFDFIRRTPDGAAGPMPLVGTGKIHPAAITPDGVMQKKAQAENFRKGAPFWS
                     IADEQGKRGKILNIPFAFPPDPLKNSLMLCALGVPDLRGTTSTYYSLSDRFTAGQLSE
                     SLGGGKRILLSFDGSDETQLPIPGPRDARFPFSDPKAYTEAMLSLKMDRKNRQGLAAS
                     GEHQVELNQGKWSEWLEFSFKMTEKDAVFGIARFFPTEIGEQIRLYMSCVQYHPEHPY
                     TALTQPEAYSAELKGRLGLYKTIGWAYDTHALRQNDLDEDAFLEDIKYTMTWREQLTM
                     DEHRRGNYDFLLSGWTATDRVGHMFWRFRDEKHPLYEEDVPDRWKQALENTYKQADAF
                     VGQMLNEITDEDTLFVFSDHGFGTWRTGFNLNSWLQENGYLSVADPKAANTGFLMGID
                     WAKTKAYSVGLSSLYINLQGRERFGTVKKDEADALAQELTARLAEVKDPATGAKVYSA
                     LYLSGEYRGEAMADAPDISLGYAATYQSGRQTSRGGVGEALFEPVTDKWSGEHASSDY
                     RHCPGVFFTNRPLEKGNPHIQDLGVTALHLLGVDMPGDLEGDMLL"
     gene            complement(2914..4782)
                     /gene="mnmG"
                     /locus_tag="PKN92_02480"
     CDS             complement(2914..4782)
                     /gene="mnmG"
                     /locus_tag="PKN92_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNZ47690.1"
                     /translation="MNTVDILVIGAGHAGIEAALASARCGFSTLLASMHLDAIGCMPC
                     NPSIGGVAKGQLTREIDALGGEMARAIDATGIQFKMLNRSKGAAVHSPRAQADRFRYV
                     DYMTEVCCTTKNLSLREMEVADFLVEDGKIHGVVATDGAEIAARAVIVCTGTYLRGLL
                     HYGMESRPGGRGNAAAANSLSSAYTRLGFTLGRLKTGTCARIHKDSVNYDLLQEQPGD
                     DNPRPFSFSTPVENFNRNFISCWITRTNENTRDIILANMDRSPLYSGKIEGIGTRYCP
                     SIEDKIVKFPDNISHHIFLEPEGLDTDEMYVNGLSTSLPPDVQYALLHSCRGLEEAEI
                     IRPGYAVEYDFIPPTQLKPTLETKAVRGLFHAGQINGTSGYEEAAAQGIYAAFNAMRY
                     LQQDQPHDISRSEAYLGVMVDDIVTRGVLEPYRLFTSRAEYRLHLRHDNADQRLAAYG
                     LERNCSAASVEAREQRIKAEIKRLRTTIAAPSPALNSLLQECGEAAAVQAAPLEQILR
                     RPGMSLEKLHRFSPPPETLDFQSREQVEIRVKYSGYLEREARMIGHFTKTEKLAIPDD
                     LDYDAISGLPRESRQRLKEVRPANFGQASRIPGVRASDVAVLHIYLVKLQRSRNGA"
     gene            complement(4826..5395)
                     /gene="folE"
                     /locus_tag="PKN92_02485"
     CDS             complement(4826..5395)
                     /gene="folE"
                     /locus_tag="PKN92_02485"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNZ47691.1"
                     /translation="MNTDRIAGLIRELLIEIGEDPDREGLLKTPDRYAKALKFLTKGY
                     HEDMNTIINGAIFQAESNNMIIMRDIELYSLCEHHMLPFFGRAHVGYIAQEKVIGLSK
                     IARIVDFYARRLQIQERLTAQIARAIMDNTRAEGVGVVLECKHLCTMMRGVEKQNSLM
                     TTSSVLGSFHDDEITRQEFLQLIGRGGQI"
     gene            complement(5385..5921)
                     /gene="folK"
                     /locus_tag="PKN92_02490"
     CDS             complement(5385..5921)
                     /gene="folK"
                     /locus_tag="PKN92_02490"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNZ47692.1"
                     /translation="MDTAVPVQAFIALGSNINPRKNIEEALNALSRKCTIAALSSFYL
                     SPPLNRPEQPDFINGVAQIKTDQPPFALKYEILRKIEADAGRCRSADKYAARPIDLDI
                     LLYGDEQISGSELNIPDPDICLRPFLFVPLLELDPLLCLPGMNTPLRALCRPEADVTL
                     KKEEEFSSYLKERFIYEH"
     gene            complement(5924..6298)
                     /gene="folB"
                     /locus_tag="PKN92_02495"
     CDS             complement(5924..6298)
                     /gene="folB"
                     /locus_tag="PKN92_02495"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012305941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNZ47693.1"
                     /translation="MTDYAHDRIFIRDLTARCILGIFPEERVKPQEVLINLVLFTDTR
                     QAGKTDCIEDTVDYKAIKNKVLELVEQSSFFLVEKLAQKIADCCLNAEGVQKVQVTVD
                     KPGALRFARSVAVEIIRTKDGQ"
     gene            complement(6291..7037)
                     /locus_tag="PKN92_02500"
     CDS             complement(6291..7037)
                     /locus_tag="PKN92_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ47694.1"
                     /translation="MNTFSLSQKNALITGASRRLGAAFARALAAADCSVVLHYNHSAK
                     DAEALASEIRAQGGKASCIQADLAEPAQVESLIPRAVAQSGPLDILINNASIFRETDF
                     SETTASSLQENIAVHATAPLLLSRAFAAQQRCGAILNMVDARIADYDQQHVPYHLSKR
                     MLADLTRIMAVEFAPRIRVNGIAPGLILPPEGKDETYLKGLAHTNLLQSFGAAQDIVD
                     AGLYLLCAAFVTGEILYVDGGRNLRGKMYD"
     gene            complement(7034..9538)
                     /locus_tag="PKN92_02505"
     CDS             complement(7034..9538)
                     /locus_tag="PKN92_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47695.1"
                     /translation="MKPDSYHRLHYFLLIAFLLIAAVLRGWFLAEIRQAPDFREMRQD
                     MSVQDYQARAMLSGDWTLPEGRSDPEIPTTPYYRPPGYCYFLAGIYYLSDGSYLAPRI
                     VNIFMGLVAIALMYLLGILLWNRRVALIAAFLMSTYWGFIFYEGEVNDPAVFVFLIPC
                     LLLALYRWQQTFHLRWILLAAIITGSYALMRPNILLFGPFMAAWILWTGFKKTTPFRI
                     AAAWVILALFTFLVIAPVTVRNYLVSGEFVPISTYFGENLYIGNCDDADGYTSWTPYL
                     QQLEGGGQFSVWEYARIVRGLGKDIGQPGLTHSEASKVFARMAIDWIREKPMAAAKVT
                     LKKAVLFWSPVEITENKVVQCEKAHYAPLKYLPGFPYVLTFFILGLGLTLLDFVGKNR
                     KKAGTCRNTPLLWLILAFIGVYWISFLPFFVNARARHPIVGLLFLFGAYGLYRFFSLL
                     LAKQCRKSILTAVLIALLFAAAHVEPYPYLPDKARWHYARADSWLREGKVDAAAAEAE
                     ALLKEDYSYYMPFRIGHAMAKKGRPALAERLLSAALSPDPQHQPAAYREDLYFHIAAI
                     QAAQGKNEAARKNFEEALALNPKDARAHNDLGILLEKEGKLEEALASYEKAVQSNPAF
                     ALAQSNLGNLLGLLGKHEEAAEAFRTAATLAPDQPEYFYNLAIHCSALNKPDEARRAY
                     EQTLLLAPKHARALNNLALLDHDEGKMEDALAKLEQAIEANPFLLIARANLGNFLIES
                     GRYTEGMAVFERALEKFPENITLCTQMSRHALSHGDLLLAEKYLSLALLLDPENEQLK
                     EQLAALHQPSDEATEPKQEEPPSNEGSTAHEETVGP"
     gene            complement(9564..10559)
                     /locus_tag="PKN92_02510"
     CDS             complement(9564..10559)
                     /locus_tag="PKN92_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNZ47696.1"
                     /translation="MSIPENNAPALSAVYAPVQKPLEEVRAILTDLWQDVLCLAQPTS
                     GGAPPKQSEGKLLRPTACLLGAGALQAPDLHSFVQLAASYETMHLASLVHDDVVDRAA
                     LRRGQSALHMYWNEHGAILGGDYLVSRALQLMVSYENTGLIARILTAVRQMSEGELRF
                     LDKSWDSVSEENCLDLARAKTATLFAASCAGAAILLAPEHYDILENYGINTGIAFQLT
                     DDMLDLTQPFTTLGKQPCGDISEKKKTLPIILLRSRMNSADLKMLDSLAGKTLSEEEC
                     LWIKEQLETLGVESAVEEYVNGYIRKGIDSLDALPPSPYRDSLAALAWYIPRRVT"
     gene            complement(10613..11359)
                     /gene="lptB"
                     /locus_tag="PKN92_02515"
     CDS             complement(10613..11359)
                     /gene="lptB"
                     /locus_tag="PKN92_02515"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNZ47697.1"
                     /translation="MSEQEALLYTDAIRKSFKKRTVVRDVSISLNAGEVVGLLGPNGA
                     GKTTSFSMIVGLLKPDQGRILFLNRDVTPMPMYRRARAGMAYLPQEPSVFQQLTVRQN
                     LQVVLENQKISRKEIRERIDLLLSEMNITHLADSPAFTLSGGERRRTEIARALAIQPK
                     VFLLDEPFAGIDPIAVADLQQIVRNLKELGIGVLITDHNVRDTLEITDRAYIINEGTV
                     LATGSPQDIAENPVVRQIYLGEKFKLDNQQ"
     gene            complement(11374..12249)
                     /locus_tag="PKN92_02520"
     CDS             complement(11374..12249)
                     /locus_tag="PKN92_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47698.1"
                     /translation="MIRWMPAFFLLCIALPVLAQTSMGGYSSMEIEAGRMKGNFATGS
                     IDEMTGGVRLKLLSEDPATKPLPVAAGSMKFTWGEGKTTPATIVMEKDVKVDHPQASI
                     TAQRAEWNFETGDLVFSGNPVVNSEQLKGLRGEKMLLNLKNNTFEVLQVRADEVPLKA
                     SEGAAAASAPSQNLTAREIKDWSALIDAIKKDAGQDRPSAGKQIMKHLNAQNQQLLVK
                     MDTALLVERKEDLLRLINSVLAQPGFYSADAWQGITLPDAVKESLVKEDLSKEDRVAV
                     NKTLLKAAYPFAFNE"
     gene            complement(12246..12845)
                     /locus_tag="PKN92_02525"
     CDS             complement(12246..12845)
                     /locus_tag="PKN92_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47699.1"
                     /translation="MKIRSLSVLLFPVLCLTAGCGTPKPAVPEIQAENPTAEAAENAL
                     QNADITMEGINLYMHRPDNHEGVPSRPELWVQAESFSIGDDKVYAFKEATAVLYGKDE
                     TEEIRLHANQGIFKQDHFAKMDGDIRMTAGAMKIFLLDLQWDRSEEDAAGVVHSDQPV
                     IIDDPDLQLNASSLRLVPDTKIFEMDNVSGVVRFGKEMS"
     gene            complement(12842..13618)
                     /locus_tag="PKN92_02530"
     CDS             complement(12842..13618)
                     /locus_tag="PKN92_02530"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNZ47700.1"
                     /translation="MLLVVDAGNSQTEVGLFDENKIIGQWRVVTTNYRTRDELHILLS
                     MLLHSIGVSRKDIRGCCIASVVPHLNSSLQQACEHTFGLEPLMVEPGIKTGIILQCDN
                     PKEVGADRIANAAGARAQCTDAMIIIDFGTATTFDVMTEKAEWLGGLILPGVQLSADA
                     LFEHCARLPRVPIKAPRSAIGKDTVTNIQAGLTYGYADMVDGLVTRITGEMGVPLYVI
                     ATGGLSRAIASVATRIDCVDPLLTLKGLCAIYERNLGKIK"
     gene            complement(13741..14577)
                     /gene="nadC"
                     /locus_tag="PKN92_02535"
     CDS             complement(13741..14577)
                     /gene="nadC"
                     /locus_tag="PKN92_02535"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNZ47701.1"
                     /translation="MDKMLRTIVANALDEDIGPEDMTTTLTVGPEIRCQVRLVSKDEG
                     ILSGIHVFQCAFDLLDAQVQDWQSLEDGSPFGRGDIIATFTGNARAVLTAERTALNFL
                     QRLSGVATLTRRFVTAVEGFPTRICGTRKTSPCIRHLEKQAIVHGGGATHRHTLFNGI
                     LIKENHITMAGGIGTAVTRAREGAHHLFRIAVEAATLADFDEAMAAGADVILLDNMDI
                     DTMAEAVRRAKLSSVLLEASGNMTLDRVRAVAETGVHYISVGMLTHSAPALDLSLLVD
                     RF"
     gene            14768..17035
                     /locus_tag="PKN92_02540"
     CDS             14768..17035
                     /locus_tag="PKN92_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNZ47702.1"
                     /translation="MPGQNTYFLTVIAVLAVAASMLMPGCELPFEETPITPVSNPAIR
                     AFEGRLAFPVGEAPYEVLSADLNGDGMEDLVTLNWLGESVSVLLGKVNGFQKKVDYPV
                     GQTPRSGVAARLTADGILSLAVLSESLEQITVLFGDGQGTFPESGIIPLTAGSRPVAM
                     ASADFNLDGAADLVVANSGTDNVSIVYSQGNKFFDAPLSIPVGKTPAGIYAGDINRDG
                     IPEILVANSGDDTVSIISLTASGYEETAVLPCPARPRRIQCADVNQDGILDICVSREF
                     SPDVAIFYGSISGAYYKTEITYSAPVSRFVVAELTSDAFPDFAAILFMEGKEEYQPSG
                     AFEVLQGGLSGTFKSLGRYGTGWGSNALIATDMNRDGRADLVTADLATNMVSLIYNRG
                     NGSFQTERHFAVPDEPGEILLADFTQDAFPDLVVTGRKTSFLYLFTNLGDASFTHTLT
                     LGLGAQALALAAGDLNGDGKTDLVVSLNQQFDLLVYINSGNGKFMPAQRLSILGTDKK
                     VLPQVRSIALGDVNGDGTPDIVTANSKVDSVSVLLNSGNAVFQAAKVIKVDNYPLDLH
                     LLDTNRDQKMDLVFLSRNDPESPTDSAEPRVCRWFGKGDGTFDLDSHIRITTGAGPRA
                     LRMGDYTGEGNMDCATIHPGDNSLYLLNGGSNGNFAVGKRFPMGIAAQDMVFVDVKKD
                     GKADMLCTNDQGSFYLRFSRGADQGFEGANNYNVRPGIGRILAADLNNDLFPDALLLD
                     RGRKAFYVLRGCSLQ"
     gene            17083..17700
                     /gene="coaE"
                     /locus_tag="PKN92_02545"
     CDS             17083..17700
                     /gene="coaE"
                     /locus_tag="PKN92_02545"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNZ47703.1"
                     /translation="MIKLIGLTGGIGSGKSQAAAFFQEEGVPVIGMDAVGHELLQHDT
                     AIKDALVGMFGSGILDGERPSREKIGRLVFQDQEALKKLNALLHPAIAQETVRRARAL
                     EQDGHGVVLIEAALFAEDYEKESWLDGLILISAPEALRIERLQKSRSMSLPEIRQRMA
                     QQADPEKKRPLADRVIHNDKSQEDLRRQVQEAARELLCRFPRRKS"
     gene            17826..19223
                     /gene="cysS"
                     /locus_tag="PKN92_02550"
     CDS             17826..19223
                     /gene="cysS"
                     /locus_tag="PKN92_02550"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNZ47704.1"
                     /translation="MALVFYNTLGRKKEAFTPITEGKAGLYTCGPTIYNYAHIGNFRA
                     YMFEDLLKRYLLFKGYEVRHIMNLTDVEDKLIRTCKETGKPLSEITRYYADAFFKDIK
                     TLDILPADVYPAATEHIHEVVEMIKTLRDRGHTYEEQGSIYFRLSTFDEYGALSGINL
                     DELQAGASGRVQADEYDAEEARDFALWKAWDEEDGDVFWETELGKGRPGWHIECSAMS
                     MKYLGAHFDIHCGGIDNMFPHHENEIAQSRCCSGEPFVNYWLHCAHLVVEGKKMSKSL
                     GNFYTLRDLLEKGIEPLAIRWVLLATHYRQPSNFAFDALAAASQSLRRIADFRLRMQE
                     IRGEGSDLQKECAACEADFVRDMDDDLNISGALGTVFTFIREVNRQADQDEVGAEGAK
                     NALALLDKLNAVTGLFAGVREEAVPEEILELVDARQKARRAKDFAASDAIRDQLAAAG
                     WVVEDTPDGPRVKKL"
     gene            complement(19248..20015)
                     /locus_tag="PKN92_02555"
     CDS             complement(19248..20015)
                     /locus_tag="PKN92_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47705.1"
                     /translation="MSPFCFSFQTDTRHKRHFHRAGTLLLFIGLAGLLFVGMFSGCSC
                     REKEDPPRTPPAAPPQESKETDLVFYAPVKTVDRAGNPLRGMLPIATRSPNAFDKPLA
                     QGKPTDADGLSSIRFALTEKIALRAWDPQLFFFPNNFFDVLPGSGNAKDPLVVVMVEA
                     GTLEAVLILPDGTPAAGENVGLMMFHPVHGPWWPAEAESNEKGEVVFPNLPPGSFILR
                     LKVESGATLETPEQYIAPGENTHMGVLYLKAPQSSSF"
     gene            complement(20012..21976)
                     /locus_tag="PKN92_02560"
     CDS             complement(20012..21976)
                     /locus_tag="PKN92_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ47706.1"
                     /translation="MNSPSHTKLPITPRDCSSDYSAWSSYFRLVGLAWHFKYRLLLSL
                     FFSLIIAASFGAMLVSVGTIIRITFYEAPAAIEESAASPADPAVKLAKDVAGINDTMR
                     RMIGWAPSSLPDRVLSLSYGMRENKMKAVTFASGLALFLTILMSSARFLQEYLAGSIG
                     THVTTGLGEAMYANLMKQSVGFFERKSSGEVLARFTNDIFMVNSGLQGVFVKLMREPI
                     KVLVFLAVALHVDWQLTLIGIGVLPLVLYLLISIGKKMRNSVKRSLQKIAGLATVVNE
                     TIKGIAIVKSFTMEEYEIGRVKAEIGRLRKFLLRMITLHAATGPLTEFTLVLGIIGFV
                     LVSGHRVETGYLDAGDLVQLYFALAMMLDPVRKLSDVNNMIQTSIASADRCFEFIDMV
                     PDILESSDAETLPALTDTIRFENVTFSYDGSKNALDGIDIEIKCGEMIALVGASGSGK
                     STFVKLLPRFYDPTGGRITFDGKDIRNATFQSLREQISYVTQENILFAESIRSNIAFG
                     RDCYTDARVREAATAAHADLFIDDLPEGFDTIIGESGSSLSGGQRQRLAIARAIIKDP
                     ALLILDEATSSLDTESERLIQEALDQFVKGRTTIVIAHRLSTIQQADRIVVLDEGKII
                     EQGSLEELLALNGAYKRLHDTQFNFSKGEQ"
     gene            22210..23310
                     /locus_tag="PKN92_02565"
     CDS             22210..23310
                     /locus_tag="PKN92_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNZ47707.1"
                     /translation="MLSLKKTVPILFCFCFLFASSACAAVPLIYCSDLFHPHDDPDDH
                     FDLACIYAIEEFDLKAIILDQGLKQKEQPGLIPVMQMNALTGREVPSVSGLEKPLVSP
                     EDTGEDQPASAQAGVETILAILRESPEPVTIITVGSLRDVAAAFNRDATLFHQKLKRL
                     YAFIGNTGGGFREYNVNLDLNAYRCIMNAGLPLYWTPCFDGGLWENNGRASYWVSPHN
                     QLLEGVPEALLNFFIYMIEKKDSADPVAAIFEPVNEREKEAFLAEKRNLWCCAVFPHA
                     ADRFYICRKGKCLALPPHSLRQGDLPAAPFSYVPVKVRLDEEGVEHCPDPDGTEILCF
                     HMTDPQNYARHMTELSVQLLHELAGLIRTSEK"
     gene            23342..25666
                     /locus_tag="PKN92_02570"
     CDS             23342..25666
                     /locus_tag="PKN92_02570"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNZ47708.1"
                     /translation="MKKWIRRLAVLLLALLLIPLAALSFIVLTYYRVVRAQPGSLPVP
                     VYAGPLGSQVNVFSGTGGYPWLCPHNSPAATVPFGMVRLAPDTASLFIKKPALNLSGY
                     YYGDNKILGFSHTRLMGAGVVEGGNFRILPTRKERMTGGQHPVMTRFSHTYETAFPGY
                     YAVRLPEEDILVELTATGHTGIHRYTFPPSVPPQILFEATSALGKGKCRESHCRLLPD
                     SGEIEASIRYYGGFSSRYDGLDLFCVLRFSRPFQSAALWNGDALYEGGFSVSGENTGA
                     CLNFSAGEPVEVRLGLSSVSVANARMNLDEEAGTLSFEEIYDRARNAWEERFGAIRVA
                     GGTESDRRIFFTALYRTFQLPTQFSDVNGDYRGFDKEIHRSEGRPYFTDMSLWDTFRS
                     VHPLYNLIARDEQNSMIRSLIEMAKIGGSLPRWPAGCGYSGSMFGTPADMMITEAWLK
                     GVRDYDIETAWFYMRRSALEGPPVDVRCARRNGLEYYLKYGYCPDDLMKGSVANTLEF
                     CWADYSLSLLAASLGKQNDAELLGRHARFYRNLWNPETGYFQPKDSAGTFSSNFKPDI
                     LTYIDFKEEYTRAFVEGSAMQWRWGVPFDGDDLVRLLGGAESFVKELEKYMEGVNPKL
                     GDWNPGPYYWHGNEPYFHAPYLFLHAGRSDLTQKWVRWILKNKYADNYVGLEGNDDCG
                     TLSAWYVLSALGLYPVAGTDRYWIGSPLFDRADLRLDEGRVLTITAENNTPENCYVKA
                     LYLNDEPHSIDFLYHHEIENGGSLRFVMSPTPAE"
     gene            25790..29164
                     /locus_tag="PKN92_02575"
     CDS             25790..29164
                     /locus_tag="PKN92_02575"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HNZ47709.1"
                     /translation="MTLCTVLLVTLLVGINAGGEILFSEETLTLPTYETMPPDKLPLF
                     YRSEEVQLAERHIYPYPFYDVQSGDKKDIQYKALILENEYLRLCVLPELGGRFYSMLD
                     KTTGYEAVYKNRVVKPALIGTLGAWISGGVEWNTPHHHRATSMIPVDYAVSKNEADGS
                     VTVWVGEYEKRSRTRWMTGLTLTPGKAYVKATFKSMNVTPLQYPALYFANAAVPVNDD
                     YQFIFPPDVELMTFHYVTEFCRWPVLNQVFQSYDYTHGEDLSWWNAAKQPVSFFVMET
                     RRDFMGGIDHGRGTGIVLIGDHRIFKGKKLWNWGKNEVQEVWDEKLTDEDGPYAELMM
                     GFYSDNQPDYNFVAPFETKYGDMYFYGIKGLRDIKEANRDLAVNLEIKGRKALIQINA
                     TLALQEVKLKLLGSGKPLLEQTIQLDPSALYETSAVLSRKTKMEDLQIVLTAADGTEL
                     LAWEQPVKKNEPFPEVYHDPLPPDEYTTSQDAYFAGLKLEQFGNTNFDYMKYYERAIA
                     LNPADVAANTRLGLIYLKRGEFDRAEGFLREAVHVVTDNHKKAKDAESLYYLGLCLMY
                     QNKVEEALDLLYRSTWDYTWTSAGYTLAAQLESRQGRFEKGLEAADRAWRANQDNVEA
                     CQCMALLYRRLKECTEAQNMQQRWLELDPMSILARNEQILLKGGYSECDPALVTDLLR
                     LMRDEPYNFIESAIRYASFGFYQDAVAILRLAADSDLPALNAYPMIYYHLAFYQDRAG
                     CTEAAAGSLAKAAGLSSTLCFPYGLESIEVLRYALSKNPEDVSALYLLGNALADYQHE
                     AAVTCWAKAAELNSADPVSFRNMAYIQAHHFRDMQKALDNIMCALSLDPSEPRYFSEA
                     HLYMSYASLSPEELEQFLEEYAGTSGHMTDIQLMQIKLNIFRGNYTEAIALLEQMQYH
                     IKEGADFNPHVYWFDAHLYDGIAAMERGSYEEAKARFLRAMEFPKNLEAERNGKIGIA
                     LYYLGLNSLASGNADEAREYFTQMSAYTYTQGWGAGDFPEIAYYQALARRQLGEKEDA
                     LQEIFRKLIADGESRLAARTDSRHITVSVDESHSGRIFLLEKEIARKNLKVSSYYMQG
                     LGYLGLGDREKAKSFFNQALQVDPLHVDAQRMLRTL"
     gene            29354..29429
                     /locus_tag="PKN92_02580"
     tRNA            29354..29429
                     /locus_tag="PKN92_02580"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29387..29389,aa:Gly,seq:ccc)
     gene            complement(29587..31050)
                     /locus_tag="PKN92_02585"
     CDS             complement(29587..31050)
                     /locus_tag="PKN92_02585"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNZ47710.1"
                     /translation="MTDKKKLTTNSGAPVVDNQNVWTAGPRGPMLLQDHWFLEKLAHF
                     DREVIPERRMHAKGSGAFGVFTVTGDITQYTRAKIFSEVGKKTELFTRFSTVAGERGA
                     ADAERDIRGFAVKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHAVKRDPRTNLRSAL
                     NNWDFWTNLPEALHQVTVVMSDRGIPATYRHMNGFGSHTFSLINAKNERFWVKFHFKT
                     QQGIKNLTDAEAEAVIGQCRESHQRDLYYSIEKGDFPRWTLYIQVMPEKEAATCPYHP
                     FDLTKVWFHKDYPLIEVGVMELNRNPENYFADVEQAAFNPANIVPGIGFSPDKMLQGR
                     LFSYGDAQRYRLGVNHHLIPVNQPRCPFHSFHRDGAMRVDGNYGSTLGYEPNSYGEWQ
                     EQPDFSEPPLDLNGPAFHWDHREDTDYYSQPAKLFNLMTPEQKEALFGNTARNMGDAP
                     DMIKIRHINNCYKADPAYAKGVADALGIPLGDCGLDK"
     gene            complement(31129..31596)
                     /locus_tag="PKN92_02590"
     CDS             complement(31129..31596)
                     /locus_tag="PKN92_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNZ47711.1"
                     /translation="MKADPTHIEERIRAFQEACRRAGAKATHQRTEIFRAVIATDSHP
                     DAQAVYRQVKERIPSISLDTVYRNLKLLAEKGFLQIAGTSHDSLRFDGNMAHHHHFSC
                     TRCGMIRDFVTDDPQGLKIPEEACAFGEVQSLHLEVKGICAACRKKLKAKDKK"
     gene            complement(32166..>33359)
                     /locus_tag="PKN92_02595"
     CDS             complement(32166..>33359)
                     /locus_tag="PKN92_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47712.1"
                     /translation="ELTYTPMEFEPVKGGPVGGTKTVIIPENGRISITMLFEEEQEHD
                     FILNKTVDGKTTQAGRFRIYSLKPDLYGMRPYKGDFHQHSHRSDGVESPPYVAGICRR
                     AGLDFTALSDHHFRGGSIEARDFYNGLPIDLRLYPGEECHFPGNPVHILSFGAEQGLT
                     ELWKNDQAACDREIAEIQAALTDLPAGVNPFHYAACVWETRKIAEFNGMSMFCHPYWV
                     TGNRYNVDERLMDHIFSTGIFDAYEVISGFDWNSLNSLDVNGIQVARYVEECAKGRRM
                     PVCGIGDTHGHERSDQFGRYYTLVFAPSSDLQDLIAGIKGFRSVAVETPKGELARAYG
                     PFRIVKYATYLLREVLPQHDELCFEEGRLMIQYASGDTSAVDRLALLQGQVARFYDKV
                     YSPAK"
BASE COUNT         7635 a         8580 c         9138 g         8006 t
ORIGIN      
        1 gcaagtgcgc gcggttcatc agaggcggcg aaaacggttc cttccggaat accccagggc
       61 atgagtccgt gatccagctc gtacccgcgg ataaacagaa tgccgtcatg catgccgttc
      121 cagcccagcg agtagtcggc aagccctttc tcatagtgat cagtcataca gatgaaatcg
      181 acatcgattt tcttcagagc cgccacaatc tccggaaaag cggcggcgga atcgtgagac
      241 agttccgaat ggctgtgaag catgccccgg tattcgtgcc agcctgtggc aatgcccggc
      301 tcgacacgct ctgctttaaa ctgagcccag gctttggctt gtttcgggaa aaggaaatat
      361 cgtttgtaaa tcccttccct gaaaaaatag cccagaatac cggcaagaat caggacaatc
      421 agaagaaaga tccggaaacc ccactttaaa caaagccgga agcgaccgca tttctttttt
      481 tcggacatga gatggatctc ctttgcgtct taccagacgc ctgcagggat aaaagaaggt
      541 acagatgatc tgcgtctttg tctgcacagc agaaaacacg gcgcagctga aaaacacagg
      601 aaggaatcgg attcacacag cgaaatgtta tgtgaacttg cttttgcaag catgccaaaa
      661 gaataaagta tcaggaaacg gtacttcaac tcttttccgg agcgaagcca gagcagccac
      721 aggaggcttt cctggcagga tcaggaggaa tgtatcggcc ggctagtatg gcagtccatt
      781 tattttgttt agatgacctt tgtttatttg aaaaatcagc ttcttcgccc ccaatgcatt
      841 gcttataaca gcatatctcc ttcgagatcg ccgggcatgt cgacgcccag cagatgcaac
      901 gctgttacgc ccaggtcctg aatgtgcggg ttcccttttt cgagcggtct gttcgtgaag
      961 aaaacaccgg ggcagtgccg gtaatcactg gaggcatgct ctccgctcca tttgtcggta
     1021 acgggctcaa agagagcttc gccgacaccg ccgcgcgagg tctgtcttcc gctctgatac
     1081 gttgcggcat agccgaggga gatgtccggt gcatcagcca tcgcctcgcc ccggtattcg
     1141 cccgacagat acagcgctga atataccttt gcgcctgttg cgggatcttt cacctcggcg
     1201 agacgtgcgg tcaattcctg cgccagagcg tccgcctcat cctttttgac agtgccgaag
     1261 cgttctctgc cctgcaaatt gatataaaga gaactgagtc cgaccgaata ggcctttgtt
     1321 tttgcccagt cgatgcccat gagaaaaccg gtattggcgg cttttggatc agcaacgctg
     1381 agatagccgt tttcctgaag ccaactgttg agattgaatc cggtgcgcca ggtaccgaag
     1441 ccatggtcgg aaaagacgaa gagggtatcc tcgtcggtga tttcgttcag catctgtccg
     1501 acaaaggcat ctgcctgctt gtaggtgttt tcaagcgcct gtttccatct gtccggtaca
     1561 tcttcttcat agagcggatg tttttcatcc cgaaaccgcc agaacatatg accgacccgg
     1621 tcggtagccg tccatccgct gagcagaaag tcataattgc cccggcggtg ttcgtccatc
     1681 gtcagctgtt cccgccaggt catggtgtat ttaatgtctt ccagaaaagc atcttcgtcc
     1741 agatcattct gtctgagcgc atgggtgtcg tatgcccagc cgatggtttt ataaagaccc
     1801 agccgtcctt tcagctcagc gctgtacgct tcgggctgcg tcagggcggt ataggggtgc
     1861 tccggatggt actggacgca ggacatatac agccggatct gctcgccgat ttccgtggga
     1921 aaaaagcgcg caataccgaa gaccgcgtct ttttcggtca ttttgaaaga gaactcgagc
     1981 cactccgacc attttccctg gttcagctca acctgatgct caccgcttgc ggcgagaccc
     2041 tgccggttct tgcgatccat tttcagagag agcatcgcct cggtgtaggc cttgggatca
     2101 gagaagggaa accgggcatc ccggggcccg ggaatgggca actgtgtctc atcggatccg
     2161 tcaaaagaga ggaggatccg tttccctccg cccagtgatt ctgaaagctg cccggcagta
     2221 aaacgatcgc tcagggaata gtaagtgctg gtggtgccgc gcagatcagg cacgcccaac
     2281 gcacagagca tgaggctgtt tttcagcggg tcgggcggaa aagcaaaggg aatattcagg
     2341 attttgcctc tttttccctg ttcatcggca atactccaga agggggcgcc tttccggaaa
     2401 ttttcagcct gcgctttttt ctgcatcact ccgtccggcg tgatggcagc tgggtgtatc
     2461 ttgcctgtac cgaccagcgg catggggccg gcggctccat cgggggtgcg gcggataaaa
     2521 tcaaaaatat tgtgcgcacc ggggtttttg caggttgcga aagaagacca ggcaacaggc
     2581 gactggggcg gaatggttgt cgtcagtgtg tagtcgccgc cgagggcttt caatcgggcg
     2641 aaatggggga gatcgcctcg ttccatcatg gcagagacaa tgctgctttc catgccgtca
     2701 tagccgagca ttatgacttt gccctgacgc agggcacggg ctttgccgat atgaaaactg
     2761 cgaccgaggg cggcaccggc aagagtacag ccgctctgtt tgagaaaggt gcgacgtgat
     2821 attccggatg tgccggcaga attctgtttc aagatgctgc atgctcctgt attggatctt
     2881 tagaaaaaga aacagtttgt ttgtacaagt tattcatgcg ccgtttcggg accgctgcag
     2941 ctttaccaga taaatatgca gcaccgccac gtcggaggcg cggacgccgg ggatgcgtga
     3001 cgcctgaccg aagttggcgg ggcgtacttc tttcagccgc tgcctgcttt cccggggcag
     3061 ccctgaaatg gcatcgtaat ccagatcatc gggaatggca agtttttccg ttttggtgaa
     3121 atgcccgatc atccgggctt cacgctccag atagccgctg tatttgactc tgatttccac
     3181 ctgctctctg ctttgaaaat ccagcgtttc gggaggaggc gaaaaacggt gaagtttctc
     3241 cagagacatg cccggacggc ggagaatctg ttccaggggc gcagcctgca cggcggcggc
     3301 ttcaccgcat tcctgcagaa ggctgtttag cgcaggagac ggcgccgcga tggtcgtgcg
     3361 cagccgtttt atctccgctt tgatacgctg ttctctcgct tcgacagagg cggcactgca
     3421 gtttctctcc agtccgtagg cggcaaggcg ctgatcggcg ttgtcatggc gcagatggag
     3481 gcggtattct gcccgggagg taaagaggcg atagggctcc agcacacccc tggtcacaat
     3541 atcatccacc ataacgccca gataagcctc actccggctg atgtcgtgcg gctgatcctg
     3601 ctggagatag cgcatggcgt taaaggcggc ataaatgccc tgtgccgccg cttcctcata
     3661 gcctgaggtg ccgttgatct gcccggcgtg gaaaagaccg cgcaccgcct tggtttccag
     3721 tgtgggcttc agctgggtgg gcgggataaa atcgtattcg acggcatagc ccggccggat
     3781 gatctccgcc tcctccagtc cgcggcagct gtggagcaag gcatactgca catcgggcgg
     3841 caggctcgtg gaaagtccgt tgacatacat ctcatcggtg tcgagccctt ccggttcgag
     3901 aaagatatga tggctgatgt tgtcgggaaa cttgacgatt ttatcttcta tactcggaca
     3961 gtaacgggtg ccgatgcctt caattttccc ggaatagaga gggctgcgat ccatatttgc
     4021 aagaatgata tcccgggtgt tttcgttggt acgtgtgatc cagcaggaga tgaagtttct
     4081 gttgaagttt tcaacgggcg ttgaaaaaga aaaagggcgc gggttgtcgt cgccaggctg
     4141 ttcctggagc aggtcatagt tgacgctgtc tttatgaatg cgggcgcagg tgcctgtttt
     4201 cagacggccc agcgtgaagc ccagacgggt ataggcactg ctcaggctgt ttgcagcggc
     4261 ggcattgcct cttccgccgg gtctgctttc catgccgtaa tgcagcagac cgcgtagata
     4321 agtgccggta cagacgatga ctgcccgtgc tgcgatttcc gcgccgtcag tggcaacgac
     4381 tccgtggatt tttccgtctt ccacgagaaa atccgccact tccatttctc taagactcag
     4441 gtttttcgtg gtgcagcaga cctcggtcat atagtcgaca tagcggaaac ggtctgcctg
     4501 agcacggggc gaatggacgg cggcaccttt gcttctgttc agcattttga actgaattcc
     4561 tgttgcatcg atggcgcgtg ccatttcacc gcccagagcg tcgatttccc gggtcagctg
     4621 cccctttgcc acgccgccga tggacgggtt gcagggcatg cagccaatgg catccaggtg
     4681 catgctggca aggagcgtgg agaagccaca gcgcgcactt gcgagagcgg cttcaatacc
     4741 ggcatgcccg gcgcctataa cgagaatgtc aacagtattc atgggaaggg tctgggaaag
     4801 gttgggatga aatgaacacg gtgacttaga tctgtccgcc gcgtccgatg agttggagga
     4861 actcctggcg ggtgatttca tcgtcatgga aactgccgag aacggacgaa gtggtcatga
     4921 gagaattctg tttttcaacg ccacgcatca tcgtgcacag atgcttgcac tcgagcacaa
     4981 cgcccacgcc ttccgcccgg gtattgtcca taatagcccg ggcaatctgg gcggtgagcc
     5041 gttcctgaat ctgcagacgg cgtgcgtaaa aatcaacgat tcgggcgatc ttgctgaggc
     5101 cgatcacttt ctcctgagca atatatccca catgggcgcg accgaagaag gggagcatat
     5161 ggtgctcgca cagactgtac agctcaatgt cgcgcatgat gatcatgttg tttgattcag
     5221 cctgaaaaat cgcaccattg ataatggtgt tcatgtcctc atgatagccc tttgtcagaa
     5281 acttcagggc ttttgcataa cggtccggcg ttttcagcag accttcccgg tccggatctt
     5341 caccaatttc aatcagcagc tcacggatga gcccggcaat acggtcagtg ttcataaata
     5401 aacctttctt tcagataaga ggaaaattct tcctcttttt tcagtgtcac gtctgcttcc
     5461 ggccggcaga gcgccctgag cggtgtgttc atgcctggca ggcagagcaa tggatccagt
     5521 tccagcagcg gaacaaataa aaagggtcga aggcaaatgt ccggatcggg gatattcagt
     5581 tctgatccgc tgatctgctc atcgccgtaa aggagaatgt caagatcaat ggggcgcgcc
     5641 gcatatttat cggcactgcg gcatcttcct gcatcggcct ctatttttct caatatctca
     5701 tattttaacg caaaaggcgg ctggtctgtc tttatttgtg ccaccccgtt gataaaatcc
     5761 ggctgttcag gtctgttcag tggcggcgac agatagaagg aggacagagc ggcgatggtg
     5821 cattttcggc tgagcgcatt cagtgcttct tctatattct ttctgggatt gatattggat
     5881 cccagtgcga taaaggcctg aacaggaaca gcggtgtcca tggttactga ccgtctttcg
     5941 tccggataat ttccaccgcg acactgcgcg caaaacggag agcaccgggt ttatcaacgg
     6001 ttacctgcac tttttgaacc ccctccgcat tcagacagca gtctgctatc ttctgcgcca
     6061 gtttttccac cagaaaaaaa ctggactgct ccaccagttc cagcaccttg tttttgatgg
     6121 ctttatagtc cacagtgtct tcgatgcaat ctgtttttcc tgcctgccgg gtgtcggtaa
     6181 aaagtaccag attaatgagt acttcctgcg gtttcacacg ttcctcagga aagataccaa
     6241 gaatgcagcg ggctgtcaga tcccggataa aaatcctgtc gtgggcataa tcagtcatac
     6301 atttttcctc gcaggttccg gccgccgtcc acataaagaa tctcacctgt gacaaaagcg
     6361 gcacacagca aatagagtcc ggcatcaacg atatcctgtg cagcgccgaa gctttgcagc
     6421 aggttggtgt gcgccagacc tttcagataa gtttcgtctt ttccttcagg aggcagaatc
     6481 agccccgggg caatgccgtt cacccggatg cgaggcgcaa attccactgc catgattctg
     6541 gtcagatcgg caagcattct cttgctcaga tggtagggca catgctgctg atcataatca
     6601 gcgatgcgtg catcgaccat attgagtatg gcgccgcatc gctgttgtgc tgcaaaggca
     6661 cggcttaaca gcagcggagc ggtcgcgtga acagcgatat tttcctgcag tgacgaagcc
     6721 gttgtttccg aaaagtccgt ttccctgaag atggatgcat tgttgatcag aatatccagc
     6781 ggacctgatt gcgccaccgc tcgcgggatg agcgattcca cctgagcagg ctccgccaga
     6841 tcagcctgga tacaggaggc ttttccgcct tgcgcccgaa tctccgatgc cagagcttcc
     6901 gcatcttttg ctgaatggtt gtaatgcagc acaacgctgc agtccgctgc ggcgagtgcc
     6961 cgggcaaagg cggcgcccag ccgccgggaa gccccggtaa tcagtgcatt tttctgtgac
     7021 agactgaaag tgttcatggg ccgactgttt cttcatgggc agtgctgcct tcatttgagg
     7081 gcggctcttc ctgcttcggt tccgttgctt cgtcggatgg ctgatgcaat gcggcgagtt
     7141 gttccttgag ctgttcattc tcaggatcca gaagcaacgc caggctcagg tatttttcgg
     7201 caagaaggag atcgccgtgg ctcagtgcat gcctgctcat ctgggtgcag agtgtgatat
     7261 tttccgggaa tttttccagc gcacgttcaa aaaccgccat tccttctgta taacggccgg
     7321 actcgataag aaaatttccg agattggcgc gggcgatgag caggaaggga ttggcttcta
     7381 ttgcctgttc gagctttgcc agagcatctt ccatctttcc ttcatcgtga tcgagaaggg
     7441 caagattatt tagcgcccgg gcatgtttcg gtgcaaggag aagtgtttgt tcatatgccc
     7501 gtcgggcttc atccggtttg ttcaatgccg agcagtggat agcgaggtta tagaagtact
     7561 ccggctggtc gggtgcgagg gtcgcagcgg tacgaaaggc ctcagcagct tcttcatgtt
     7621 ttcccagaag gcccagcaga tttccaagat tgctttgagc cagagcaaac gccgggttac
     7681 tctgcacagc cttttcataa gaagcgagcg cttcctccag ctttccttcc ttttccagca
     7741 atatcccgag atcgttgtgt gccctggcat ccttcggatt cagtgccagt gcttcttcaa
     7801 agttttttcg cgccgcttca tttttcccct gtgccgcctg aatcgctgca atgtgaaaat
     7861 agagatcttc ccggtaggct gcaggctggt gctgcgggtc gggactcagg gcagcgctga
     7921 gcagccgctc cgccagagcc ggacgtcctt tttttgccat ggcgtgaccg atccggaagg
     7981 gcatgtaata agaataatct tctttcagca gggcttccgc ttccgcagcg gcagcgtcca
     8041 ctttgccttc acggagccag gaatcagcac gggcatagtg ccagcgtgcc ttatcaggca
     8101 ggtagggata gggttccaca tgtgccgcag caaacaaaag ggcgatgagc accgctgtaa
     8161 gaatactttt ccgacattgt tttgcgagga ggagcgaaaa aaaacgatac aatccgtaag
     8221 caccgaaaag gaaaagcagc ccgacaatgg gatggcgtgc acgggcattg acaaaaaaag
     8281 gcaggaagga gatccagtac actccaataa atgcgagaat cagccagagc aggggcgtgt
     8341 ttcggcaggt gcccgccttt tttctgtttt tgccgacaaa atccagcagg gtgagaccca
     8401 gtcccagaat aaaaaaagtc agtacataag ggaagccggg cagatatttc aggggtgcgt
     8461 aatgggcttt ttcgcattgc accactttgt tctccgtgat ttccacgggc gaccagaaca
     8521 gcacggcttt tttcagggta acctttgccg ctgccatcgg tttttcacgg atccagtcta
     8581 tagccatgcg tgcaaaaact tttgacgcct cagaatgagt cagaccaggc tgcccgatgt
     8641 ctttgcccag gccgcggaca attctggcgt actcccagac ggaaaactga ccgccgcctt
     8701 ccagctgctg gaggtaaggc gtccaggaag tgtagccgtc cgcatcatcg caattgccga
     8761 tatatagatt ttcgccgaag taggtggaaa tgggcacaaa ttctccggat accagataat
     8821 tgcggacggt gacaggtgca atgacaagaa aagtgaaaag cgcaagaatg acccaggcgg
     8881 cggcaatcct gaaaggagtt gtttttttga atccagtcca gagaatccac gccgccataa
     8941 aaggaccgaa cagaagaata ttggggcgca tcagggcata ggagccggtg atgattgctg
     9001 ccagaagaat ccatcggaga tgaaaagttt gctgccagcg ataaagtgcc agcagaaggc
     9061 agggaatcag aaacacaaaa acagccgggt cgttcacctc tccttcatag aaaatgaacc
     9121 cccagtaggt gctcataaga aaagcggcta tcagggcaac tctgcggttc cagaggagga
     9181 taccaagaag atacataagt gcaatggcga cgagtcccat gaagatattg acgatgcggg
     9241 gcgccagata actgccgtca gaaagataat agatgcctgc caggaaatag cagtagccgg
     9301 gagggcggta atagggcgtg gttggaattt cgggatctga gcgcccttcc ggcagtgtcc
     9361 agtcgccgga gagcatggcg cgtgcctgat aatcctgcac agacatatcc tgccgcatct
     9421 cgcggaaatc gggtgcctgg cggatctccg ccagaaacca cccgcgcaaa acggccgcta
     9481 taaggaggaa ggcaataagc aggaaataat gaagtctatg ataactgtcc ggtttcacga
     9541 tctctcctga gcagagacag gtttcaggtt acgcgccggg ggatatacca ggcaagtgcg
     9601 gcgaggctgt cgcggtaggg agacggcggc agggcatcca gactgtcaat gcctttgcgg
     9661 atgtacccgt ttacatattc ctctacggcc gattccacgc ctagtgtttc caactgttct
     9721 tttatccaaa ggcattcttc ttcggaaagt gttttccctg ccagactgtc cagcattttc
     9781 aggtcggcac tgttcatgcg gcttcgcagc aggatgatag gcagtgtttt ctttttttcg
     9841 ctgatgtcac cgcagggctg tttgcccaga gtagtgaacg gctgcgtcag atcgagcatg
     9901 tcatcggtga gttgaaaggc aatgccggta ttgatgccgt agttttccag aatatcatag
     9961 tgctccgggg cgagcagtat cgcggctccg gcacaggacg ctgcaaaaag agttgccgtt
    10021 ttagcccgtg ccagatcgag gcagttttcc tcactgaccg aatcccagga cttgtcgagg
    10081 aaacgaagtt ctccttcgga catctggcgt acggcggtga ggattcgggc gatgagaccc
    10141 gtgttttcat aggaaaccat caactgcagc gcccgggata ccagataatc gccgccgagg
    10201 atagcgccat gttcgttcca gtacatatgc agcgccgatt gaccccgccg gagggcagcg
    10261 cggtcgacca cgtcatcatg gacgagcgat gccagatgca tggtctcata agaagccgcc
    10321 agctgcacga agctgtgaag atccggcgcc tgcagtgcgc cggcacccag cagacaggcc
    10381 gtaggtcgca gcagttttcc ctccgattgt tttggtgggg cgcccccgga ggtcggctgg
    10441 gcgaggcaca gcacatcctg ccacaggtcc gtcagaatcg cccggacctc ttccagcggt
    10501 ttctgcacgg gagcatagac ggcgctcagc gccggcgcat tattttcagg tatggacatg
    10561 gttttgtttt tgttggcctt ttaaccttcc cgaagggaga agaaaggtct tctcactgct
    10621 ggttatccag tttgaatttt tcgcccaggt aaatctgccg gactacaggg ttttccgcaa
    10681 tatcctgcgg actgcccgtc gccagaacag tgccctcatt gatgatataa gcacgatccg
    10741 ttatttccag tgtgtcacgg acattgtgat cggtaattaa aacgccgatg cccagttctt
    10801 tcagattccg gacaatctgc tgcagatccg ccacggcaat gggatcaata cctgcaaaag
    10861 gttcatcgag cagaaaaact tttggctgaa tggcaagagc acgggcgatt tccgtgcggc
    10921 gccgttctcc tccggagagt gtaaaggcag ggctgtcggc aaggtgggta atgttcatct
    10981 cggagagaag aaggtctatc cgttctctga tctctttgcg gctgattttc tggttttcca
    11041 gcacaacctg caggttctgg cgcaccgtca gctgctgaaa aacggaaggt tcctgcggaa
    11101 ggtatgccat gcctgcccgt gcccgccgat acatgggcat gggggtcacg tcacggttga
    11161 ggaaaagaat gcgcccctga tcaggtttga gcagacctac gatcatgctg aatgaagtgg
    11221 tctttcctgc gccgttgggt ccaagcagac cgaccacttc tccggcattg aggctgatgg
    11281 acacgtcacg caccaccgtc ctttttttaa aagactttct gatagcgtcg gtgtaaagaa
    11341 gggcttcctg ttctgacacg tccaagcttt ctgttattcg ttaaatgcaa agggatacgc
    11401 cgctttaagc agcgtcttat ttactgctac tctgtcttct ttggaaagat cttccttcac
    11461 gagcgattct ttcaccgcat cgggaagcgt aatgccctgc caggcatcgg cgctgtaaaa
    11521 accgggctga gccagtacgc tgttgatgag ccgaagaaga tcctcttttc tttccaccag
    11581 cagggcagta tccatcttta ccagtagctg ctgattctgt gcattcagat gcttcatgat
    11641 ctgcttgcct gccgaaggac gatcctgccc ggcatctttt ttgatggcat cgatgagtgc
    11701 ggaccagtct ttaatttccc gggcagtcag gttctgggag ggggcagatg cagcagcagc
    11761 accttctgag gctttcagcg gcacttcatc cgcccgaacc tgcagcactt caaaagtgtt
    11821 gtttttcagg ttgagcagca ttttctcgcc acgaagccct ttcaactgct cgctgttgac
    11881 gaccggattg ccggaaaaaa caagatcgcc tgtttcaaaa ttccattccg cccgctgcgc
    11941 agtgatggat gcctgcggat gatccacttt cacgtctttt tccatcacga tggtagcggg
    12001 agtggttttg ccttcgcccc aggtgaattt catgctgcct gctgcaacgg gaaggggctt
    12061 tgtcgccgga tcttccgaaa gaagtttcaa tcgtactccg cccgtcattt cgtcaatgga
    12121 ccctgtggca aagttgcctt tcatgcgccc cgcttcaatc tccatggacg aataaccgcc
    12181 catggatgtc tgcgccagta cgggcagtgc aatgcaaagg aggaagaagg cgggcatcca
    12241 tcgtatcatg acatttcctt tccaaagcgg acaacgccgg acacattgtc catctcgaat
    12301 atttttgtat caggcacaag ccggagggaa gaggcattca gctgcaggtc aggatcatcg
    12361 atgattacag gctggtctga atgcaccaca ccagcggcat cctcttctga tctgtcccat
    12421 tgcagatcca gcagaaagat tttcatggca ccggctgtca tccggatatc tccgtccatt
    12481 ttggcaaagt gatcctgctt gaaaataccc tgattggcgt gcagccgtat ctcttctgtc
    12541 tcatctttgc cgtaaagtac agcagtcgcc tctttaaaag cgtacacttt gtcatcaccg
    12601 atggagaaag actccgcctg cacccacaat tccggacggc tgggaacccc ttcatgattg
    12661 tccgggcggt gcatgtataa attgattcct tccatggtta tatcggcgtt ctgcaaagca
    12721 ttttcggctg cctccgctgt cgggttttct gcctgtattt cagggacggc aggcttgggg
    12781 gtgccacagc ctgcagtcag gcaaagcacc ggaaaaagaa gaactgagag agaccgtatt
    12841 ttcattttat tttgccaagg ttccgttcat agatggcgca caggcctttg agcgtcagaa
    12901 ggggatccac gcaatcgatg cgtgtggcaa ccgaagcgat tgcgcgggac agcccccccg
    12961 ttgcaattac atagaggggg acacccattt cccctgtaat gcgcgtcacc agaccgtcca
    13021 ccatatcagc ataaccgtag gtgaggcccg cctgaatatt ggtaacggtg tcttttccga
    13081 tagcgcttcg gggcgcttta atcggaaccc gcggcagccg cgcacagtgt tcaaaaagtg
    13141 cgtctgccga cagctgcaca cccggaagga tcagcccgcc cagccactcc gctttttcgg
    13201 tcatcacatc aaaagtggta gcagttccga agtcgataat aatcatggca tcggtacatt
    13261 gcgcccgggc tcctgccgca ttggcgatgc ggtcggcacc cacctctttg ggattgtcgc
    13321 actgaaggat aatgcctgtt ttgatgcccg gctccaccat gagcggctcc agcccgaaag
    13381 tatgctcgca tgcctgctgc agtgagctgt tgagatgggg caccacactg gcgatacagc
    13441 agccccggat atctttccgg gaaacgccga tactgtgcag cagcatgctc agaagaatat
    13501 gaagttcatc ccgggtccgg taattggtag tgacgacacg ccactgcccg atgattttat
    13561 tctcatcaaa aagcccgact tctgtttgtg aattacctgc atcaacgaca agcaacatgt
    13621 tttttcatac ctgaaccgca ctgccgagcc cgcgtaacgg gtgcggcagg tctgtgctac
    13681 cgggcgccca actgcgcccg ccctgagact gacccgcata aaaacaagca gaaccggaat
    13741 ctaaaaccgg tccaccagca gagacagatc caatgccggt gctgaatggg tcagcattcc
    13801 caccgagata taatgcacgc ctgtttccgc cacggcacgc acccgatcca gcgtcatgtt
    13861 accgctcgct tccagaagaa cggaagacaa tttcgcgcgc cggacggctt ccgccattgt
    13921 atcgatgtcc atattatcca gaagaatgac atccgcaccg gcagccatag cttcatcaaa
    13981 gtccgccagt gtcgctgctt ccacggcgat acggaaaaga tggtgtgctc cttcccgcgc
    14041 ccgggtaaca gccgtgccga tgccgcctgc catggtgata tggttttcct tgatcagtat
    14101 gccgttaaat agcgtgtgcc ggtgtgtcgc accgccgcca tgtacaatcg cctgtttctc
    14161 aagatggcgg atgcagggac tggttttccg ggtgccgcag atcctggtgg ggaacccctc
    14221 aacagcagtc acaaaacgcc gggtcagcgt ggcaaccccc gaaagccgct gcagaaaatt
    14281 caatgccgtg cgctcggcgg tcagtacagc gcgggcgttg ccggtgaagg tggcgataat
    14341 atcgcctctg ccgaaggggc tgccgtcttc cagagactgc cagtcctgca cctgggcgtc
    14401 cagcaggtca aaggcgcact gaaaaacatg tataccgctt aaaatgccct cgtcttttga
    14461 cacaagccgt acctgacatc gaatttcagg acctactgtc agagttgtcg tcatgtcttc
    14521 cggcccgata tcttcatcaa gagcgtttgc gacaatggta cgtagcattt tatccataat
    14581 tctgttaaat ccgtttaaag caagagttta gcacaggcag gcaggtaaac gcatgttcac
    14641 tgcggctccc gaatggagta tactgtatca aaagaccttt accgcggtat agtctgctat
    14701 gcggtctttt ccgctgcgca gcaggaattc ccgttgcttt tccctaaatt gcggagtttt
    14761 atatgccatg cccggacaaa acacttattt tctgacagtt attgccgttt tggcggttgc
    14821 cgcgagcatg ctcatgcccg gctgcgaact gccttttgaa gaaacgccga tcacccccgt
    14881 cagcaatccc gctatccgcg cttttgaagg acggcttgct ttccctgtag gcgaagcccc
    14941 ttatgaagtt ctgtccgctg atcttaacgg agacggcatg gaagacctgg tcacattgaa
    15001 ctggctgggg gagagcgtgt cggtgctgct gggcaaagtg aacgggtttc agaaaaaagt
    15061 ggattatccc gtcgggcaga cgccccgttc aggcgtggcg gcacggctga ccgctgacgg
    15121 cattctttct cttgccgttc ttagcgaatc gctggagcag attacagtgc tcttcggtga
    15181 cgggcagggc acttttcctg agtcggggat tattccgctg accgcaggct cccgccctgt
    15241 cgcgatggca tctgccgatt tcaatctgga tggcgccgcc gatcttgtcg tggcgaacag
    15301 cggaacagac aatgtatcaa ttgtttacag tcagggcaac aagttttttg atgcgccttt
    15361 atccattcca gtgggcaaaa cacctgccgg tatatatgca ggcgacatta accgtgacgg
    15421 aattcccgag attcttgtag cgaacagcgg tgatgataca gtttcgatta tttcacttac
    15481 ggcgtcgggc tatgaagaaa cagctgttct gccctgcccg gcacgccccc gcagaatcca
    15541 gtgcgctgat gtgaatcagg acggcatact ggatatttgt gtttcccgtg aattttcccc
    15601 ggacgttgcg atattttacg gaagtataag cggggcctat tataaaacgg aaatcactta
    15661 cagtgcgcct gtttcccgtt ttgtcgttgc ggagctgacg tcggacgctt ttccggattt
    15721 tgcagccatt ctttttatgg aaggcaaaga agagtatcag ccttccggtg cctttgaggt
    15781 gcttcagggc ggactttccg gcacctttaa atctctgggg cgatatggaa cgggctgggg
    15841 ctccaatgcg ctgattgcga cagatatgaa tcgggacggc cgtgcagacc tggtgacggc
    15901 agacctcgcc acaaatatgg tgtctctcat ttataaccgg ggcaacggtt cttttcagac
    15961 ggagaggcac tttgctgtgc cggacgagcc cggagagatc ctgcttgctg attttacgca
    16021 ggatgctttt ccggatctgg ttgtaacagg cagaaagacc agttttctct atctgttcac
    16081 caatcttggc gacgcttcct ttacccatac actgacgctg gggcttgggg cgcaggcact
    16141 ggcactggcg gcaggggatt tgaacggaga cgggaagacc gatctggtcg tgtcgctgaa
    16201 ccagcagttt gacctgctgg tttacatcaa ttcgggtaat ggaaagttta tgcctgcaca
    16261 gcgcctgagt attctcggta ctgataaaaa agtgctgcct caggtgcggt ccattgcgct
    16321 gggcgatgtg aacggagacg gcacaccgga tattgtgaca gccaacagca aggtggactc
    16381 cgtttctgta ctgctgaaca gcggcaatgc tgtctttcag gcggcaaaag tgatcaaagt
    16441 ggacaattat cctctggatc ttcatcttct cgacacaaac cgggatcaga aaatggatct
    16501 tgtttttctg agcagaaacg accctgaatc tcccacagac agtgctgaac cccgggtctg
    16561 ccgatggttc ggtaaaggcg atggtacctt tgatctggat tcgcatatac gcatcacgac
    16621 cggtgccggc ccgcgcgctt tgcggatggg agattacacc ggggaaggca acatggattg
    16681 cgcaacgata catccgggcg acaacagcct ttatctgttg aatggcggga gtaacggcaa
    16741 ttttgccgtt ggaaaacgtt ttcccatggg catcgccgct caggatatgg tttttgtaga
    16801 tgtgaaaaaa gacggcaagg ctgacatgct gtgcaccaac gatcagggat ccttttatct
    16861 tcgtttcagt cgcggcgccg atcagggatt cgaaggggcc aataattaca atgtccggcc
    16921 cggcatcggc agaatcctcg ccgctgatct gaacaatgat cttttccccg atgcgcttct
    16981 tcttgatcgc gggcgcaaag cattttatgt tttgcgcggt tgttctcttc agtaattatt
    17041 gttatgcctt tttttatttt gtgagagcac ccggaactgg tgatgattaa actgatcgga
    17101 ctgacaggcg gcatcggcag cggaaaatcg caggcggcgg ctttttttca ggaagaaggc
    17161 gtgcctgtga tcggcatgga cgctgttggt cacgagctgt tgcagcatga taccgctatc
    17221 aaagatgctc ttgtcggtat gttcggttcc gggattcttg acggagaaag accgtcaaga
    17281 gagaaaatcg gcagattggt ttttcaggat caggaagcgc tgaaaaaact caatgccctg
    17341 ctgcatcccg ccattgcaca ggagaccgtc aggcgcgccc gtgctcttga gcaggacggt
    17401 catggagtcg tgctgataga ggcggcgctt ttcgccgaag attacgagaa agaaagctgg
    17461 ctggacggtc tgattctgat cagtgcaccg gaagcgctcc gtatagagcg gctgcagaaa
    17521 tcgcggagta tgagcctgcc ggagattcgg cagcgcatgg cacagcaggc cgatccggag
    17581 aaaaaaagac ctctcgccga tcgggtgatt cacaatgaca aatcacagga agatctccgc
    17641 agacaggtgc aggaagctgc ccgggaactg ctctgtcgtt tcccgcggag aaaaagttaa
    17701 gtgccctgtc gcaaagccct tttgggtact gatgcgctct tttagtatca ttcccggaag
    17761 gcggtgcgcc ggcaccggct gacttcaggt tttattttca taggaaaaat caggaaatca
    17821 cagcaatggc acttgttttc tataacacgc tcggaagaaa aaaagaggct tttacgccga
    17881 ttacggaagg gaaagcagga ctctatacct gcggtccgac tatctataat tatgcgcata
    17941 tcggtaattt ccgtgcctat atgtttgagg atctgctcaa gcgctatctt cttttcaagg
    18001 ggtacgaagt gcgtcacatc atgaacctca ccgatgtgga ggataaactg atccgcacct
    18061 gcaaagaaac aggaaagccc ctttctgaaa ttacccgata ctatgctgat gcctttttca
    18121 aagatatcaa gaccctggat attctgcctg cggatgtata tcccgccgcc accgaacata
    18181 ttcatgaagt ggtggagatg atcaaaacac tccgggatag agggcatacc tacgaagagc
    18241 agggcagcat ttatttccgg ctgagcacat ttgatgaata tggcgctctc agcggtatta
    18301 atctggatga actgcaggcc ggtgccagcg gaagagtgca ggcggatgag tacgatgcgg
    18361 aagaggcacg tgattttgcc ctgtggaaag cgtgggacga agaagacggt gatgttttct
    18421 gggaaacaga actgggcaaa ggcagaccgg gctggcatat tgaatgctcc gccatgagta
    18481 tgaaatatct gggtgctcat ttcgatattc actgcggcgg cattgacaac atgtttcctc
    18541 atcatgaaaa tgaaattgcg cagtcacgct gctgcagtgg tgagccattt gtgaattact
    18601 ggcttcattg cgcccatctg gttgtggaag ggaagaagat gtccaagtcg ctgggcaact
    18661 tttatacgct ccgcgatctt ctcgaaaagg ggattgaacc tctggcaatc cgatgggtgc
    18721 tgctggcaac tcattatcgt cagcccagca acttcgcctt tgatgcactc gcagctgcaa
    18781 gtcagtctct gcgccgcatc gccgatttcc ggctgcgcat gcaggaaatc aggggagagg
    18841 ggagtgatct tcagaaagaa tgtgccgcct gcgaagcgga ctttgtccgg gatatggatg
    18901 atgacctgaa tatttccggc gccctgggca cggtctttac ttttattcgt gaggtgaacc
    18961 ggcaggcgga tcaggatgaa gtaggcgctg aaggcgcaaa gaatgcactg gcgcttctgg
    19021 ataagctgaa tgcggttacc ggtctttttg cgggagtccg cgaagaggcg gtgcccgaag
    19081 aaattctgga actggtcgat gcccggcaga aggcgcggcg cgccaaagac tttgccgcct
    19141 ccgatgctat ccgcgatcag ctggcggcgg cagggtgggt ggtggaagat acgcctgacg
    19201 ggccccgggt caaaaaactc tgattttgaa attttccgcc gacagcctta aaaagaggag
    19261 gactgcggag ctttcaggta gagcacgccc atatgggtgt tttcgcctgg agcgatatac
    19321 tgctccggag tttccagtgt tgcgccggat tccactttca gacgcaggat gaaacttccc
    19381 ggcggcagat tggggaacac cacctcaccc ttctcattgc tttccgcctc tgcaggccac
    19441 cagggaccgt gcaccgggtg gaacatcata agtccgacat tttctccggc ggcgggggtt
    19501 ccgtccggaa gaatcagtac cgcttccaac gtgcccgcct ccaccatcac gactaccagt
    19561 ggatctttgg cattgcccga gccgggaagc acatcaaaaa agttattcgg aaaaaagaaa
    19621 agctggggat cccaggcacg tagcgcaatc ttttccgtta gtgcgaagcg gatgctgctg
    19681 agcccatccg catcggtcgg cttgccctgt gccagaggct tgtcaaaggc gtttggcgac
    19741 cgcgtggcga tggggagcat gccccgcagc ggattgcccg cacggtctac cgttttcacg
    19801 ggtgcataaa aaacaaggtc ggtttctttg ctttcctgag gcggagcggc aggcggcgtc
    19861 cggggcggat cttctttttc tctgcagctg cagccgctga acatgccgac aaaaagcaga
    19921 cctgccagac cgataaacag aaggagggtt ccggcacggt ggaagtgtct tttatggcgt
    19981 gtgtctgtct gaaaagaaaa acagaaagga ctcactgttc gccctttgaa aaattgaatt
    20041 gcgtgtcatg aagccgctta taggcaccgt ttaacgccag cagttcttcc aaagagccct
    20101 gctcgataat tttcccctcg tcaagcacaa caatgcgatc agcctgctga atggtagaca
    20161 gacggtgggc tatgacgata gtagtgcgcc ctttaacaaa ctgatccagt gcctcctgga
    20221 tcagccgctc gctttccgtg tccaggctgg acgtcgcctc atccagaatc aggagcgccg
    20281 gatctttgat gatagcgcgg gcaatggcga ggcgctgccg ctgtccgccg gaaagagaag
    20341 agcccgattc tccgatgatg gtgtcgaagc cttcgggcag atcatcaatg aaaaggtcgg
    20401 catgcgccgc tgtcgccgct tcacgcaccc gtgcatcagt gtaacagtca cgtccgaaag
    20461 cgatgttgga acggatgctt tcggcaaaaa ggatgttctc ctgggttaca tagctgatct
    20521 gttcgcgcag actttggaac gtggcattcc ggatgtcttt gccgtcaaag gtgatccgcc
    20581 ctccggtagg gtcgtaaaag cgggggagca gtttgacaaa ggtgcttttg ccggaaccgc
    20641 tggcaccgac cagagcaatc atttcaccgc attttatttc aatgtctatg ccgtccaggg
    20701 catttttgct gccgtcgtaa ctgaaggtta cattttcgaa acggatggtg tcggtaagcg
    20761 ccggcaatgt ttccgcatcg gatgactcca ggatatccgg aaccatgtcg atgaattcga
    20821 agcaccggtc tgcgctggca atgctggtct ggatcatatt gttgacatca gaaagttttc
    20881 gcacgggatc cagcatcatg gcaagagcga aatagagctg caccagatca cccgcatcaa
    20941 gatagcccgt ttccacgcgg tgcccgctga caaggacaaa accgataatg ccaagcacaa
    21001 gcgtaaattc ggtgagcgga ccggtggcgg cgtgcagtgt aatcatgcgc agcagaaatt
    21061 tgcgcaatcg tccgatttcc gctttcaccc gcccgatctc atattcttcc atggtgaaac
    21121 ttttgacaat ggcgataccc ttgatggtct cgttgaccac cgtcgccagt cccgctattt
    21181 tctgcagcga acgctttaca ctgttccgca tttttttgcc gatactgatc agaagataaa
    21241 ggacgagagg gagcacgccg atgccgatga gcgtcagctg ccagtccaca tgcagtgcca
    21301 cggcaagaaa cacaagcact ttaatgggct ctcgcatgag ctttacaaag acaccctgca
    21361 gaccgctgtt gaccataaag atatcatttg taaaccgcgc cagcacttct cccgagcttt
    21421 tccgctcaaa aaagcccacc gattgtttca tcagattggc gtacatggct tcacccagcc
    21481 ccgttgtcac atgcgtgccg atggaacctg cgagatactc ctgcagaaaa cgggcggagc
    21541 tcatgagtat agtcagaaac aacgcaagtc ctgatgcgaa agtgaccgcc ttcattttgt
    21601 tttcgcgcat cccgtaggaa agggacagca ctctgtcggg cagggaagaa ggcgcccatc
    21661 cgatcatccg gcgcatggta tcgttaatgc ctgccacatc ctttgcaagc tttacagccg
    21721 ggtctgcggg agaagcggcg ctttcctcaa ttgcggcggg tgcctcgtaa aaagtgatgc
    21781 ggatgatggt gccgacactg accagcatgg cgccgaaaga agcggcaatg atgagtgaga
    21841 agaaaagaga aagcagcagc cggtatttaa aatgccacgc cagacctacc agccggaaat
    21901 aggatgacca ggcggaatag tcggacgagc agtcacgcgg ggtaatgggc agcttggtgt
    21961 gtgacggaga attcatgaag ccttactctc taggggggat tgaatatacg aatagtagca
    22021 cagcctttat gatttcggca aaccgctttc tttcgcactg cccgcaaaaa cagggatgca
    22081 gaagaaaaag gaagtgtctg ttttcagaaa tttcagaggt tatttgagag gcagccgccg
    22141 gatgttttct tttacacaag cagggtttta taataggggc tgctcacatc ctctatgcgg
    22201 gagtcgctaa tgttgtccct gaaaaaaact gttccgatcc tcttctgttt ctgttttctt
    22261 ttcgcatctt cggcttgcgc ggctgttccg ttgatttatt gcagtgattt gtttcaccct
    22321 cacgatgatc cggacgatca ctttgatctg gcgtgtatct atgctattga agaattcgac
    22381 ctgaaggcga taattctgga tcagggtctg aaacagaaag agcagcccgg tttaattccg
    22441 gtgatgcaga tgaacgcatt gacaggaaga gaggttcctt ccgtttccgg gctggaaaaa
    22501 ccgctggttt ccccggagga taccggagaa gaccaaccag caagcgcaca ggcaggtgtc
    22561 gaaaccatcc tggcgattct tcgtgaaagc cccgagccgg tcaccatcat tacagtaggc
    22621 agccttcggg atgtggcggc agcttttaac cgggacgcca cattgtttca tcagaaactg
    22681 aaacgtctct atgcatttat cggaaacacc ggcggcggtt ttcgagaata taatgtgaac
    22741 cttgatctca atgcctatcg ctgtatcatg aatgcaggtc tgccgctcta ttggacgccc
    22801 tgttttgatg gcggtctttg ggaaaacaac gggcgggcat cctactgggt ctcgccgcat
    22861 aaccagctgc tggagggcgt gcctgaagcg ctgcttaatt tttttatcta catgatcgaa
    22921 aaaaaagaca gtgctgatcc cgttgccgca atttttgaac cggttaacga aagagagaaa
    22981 gaagcctttc ttgcggaaaa gcggaactta tggtgctgcg ctgtttttcc ccatgccgca
    23041 gaccgcttct acatctgccg gaaaggaaaa tgtcttgccc tgccgccgca cagtctccgg
    23101 cagggagacc ttcctgcagc gccattcagt tatgttcctg taaaagtccg gctcgacgaa
    23161 gaaggcgtgg aacattgccc ggacccggac ggcacagaaa tcctttgctt tcatatgacc
    23221 gatcctcaaa actatgcccg tcatatgacg gagctttccg tgcagctgct tcacgagctc
    23281 gccggcctta tacgcacctc cgaaaaataa tctgcgaaca atacctacaa ggacaccttc
    23341 cgtgaaaaaa tggattcgcc gtctggctgt tttattgctt gccctgcttt taatcccttt
    23401 ggcggcactg tcttttatag tgctcaccta ctaccgggtc gttcgtgcac agccgggcag
    23461 cctgcctgtt ccggtctatg ccggtcccct cggctcacag gtaaatgtat tcagcggaac
    23521 aggcggatac ccctggcttt gcccgcataa ttctccggcg gcaacagttc ctttcggcat
    23581 ggtgcggctg gcgcctgata cagcttccct ctttatcaaa aaaccagcac ttaacctttc
    23641 gggatattac tacggcgaca ataaaatatt ggggttcagc cacacccggc ttatgggcgc
    23701 cggagttgtc gaaggaggga atttcaggat tttgcccaca cgaaaagaac ggatgacggg
    23761 cggacagcac ccggttatga cccgtttcag ccacacctac gaaacagcct ttcccgggta
    23821 ttatgccgtt cgcctgccgg aggaggatat tctggttgag ctcactgcca ccgggcacac
    23881 cggcattcac cggtacacct ttcctccttc tgtgccccca cagattcttt ttgaagcgac
    23941 cagcgcactg ggtaaaggga aatgcagaga gagccattgc cgtctccttc cggacagtgg
    24001 cgaaatcgaa gcatcaatcc gttattacgg cggtttttcc agccgctatg atgggcttga
    24061 tcttttctgc gtccttcggt tcagcagacc ttttcaatcg gctgccctct ggaacgggga
    24121 tgctctttat gaaggcggct tttctgtttc cggcgaaaac acaggcgcct gtctcaactt
    24181 ttctgctggt gagcccgtgg aagtgcgact gggtctttcc agtgtcagcg ttgccaatgc
    24241 ccggatgaac ctggatgaag aggcaggcac tctgtctttt gaagaaattt acgaccgtgc
    24301 ccgaaatgcc tgggaggaac gcttcggcgc aatccgtgtt gccggaggaa cagaatcaga
    24361 ccggcgcatt ttctttaccg ctctttacag aactttccag ctgccaacgc aattcagtga
    24421 tgtgaatggt gactatcgcg gatttgataa agagattcat cgcagcgaag gcagacccta
    24481 ctttaccgat atgtctcttt gggacacttt ccgttctgtg catccccttt acaatcttat
    24541 tgcccgggac gagcagaaca gcatgatcag atctttgatc gaaatggcaa aaataggggg
    24601 gagcctgccc cgctggcctg ccggctgcgg ctattccgga agtatgttcg ggaccccggc
    24661 cgatatgatg attaccgagg cgtggctgaa gggtgtccgg gactatgata ttgaaacagc
    24721 gtggttttat atgcgaagaa gtgcgctgga agggccgcct gtcgatgtcc gatgtgcccg
    24781 gcgcaacggt cttgaatact atctcaaata cggatattgc cccgatgatc tgatgaaggg
    24841 ctctgtagcc aatacactgg agttttgctg ggcggattat tccctctcgc tgctggctgc
    24901 atctttgggc aagcaaaacg atgcggagct tctgggcagg catgcccggt tttatcgcaa
    24961 cctgtggaat ccggaaacag ggtatttcca gccgaaagac agtgccggaa ctttttcgtc
    25021 caatttcaaa cccgatattc tgacctatat cgattttaaa gaagaatata cccgtgcttt
    25081 tgtggaagga agtgctatgc aatggcgctg gggcgtgcct ttcgatggcg atgatctggt
    25141 cagactcctg ggcggtgcag agtcctttgt aaaagagctg gaaaagtaca tggaaggcgt
    25201 gaatccgaaa ttgggtgact ggaatcccgg gccctattac tggcatggca acgagccata
    25261 ttttcatgcg ccctatcttt tcctccatgc gggaaggtcc gaccttaccc agaaatgggt
    25321 gcgctggata ttgaaaaata agtatgccga caattatgtc gggctcgaag ggaacgacga
    25381 ctgcggtact ttgtcggcgt ggtatgtcct gagcgcactc gggctctatc ctgtcgccgg
    25441 tacagaccgg tactggattg gctcacctct ttttgaccgg gcggatctta gactcgatga
    25501 gggcagggtg ctcacgatta cggcagaaaa caacacgccg gagaactgtt atgtgaaggc
    25561 actttacctc aacgatgaac cgcatagcat tgattttctg taccaccatg aaattgaaaa
    25621 cggcggctcg ctgcgttttg tcatgtcgcc gacacctgcg gaataaagga gctggactta
    25681 tacaatattc gggaaatttt atttgaccac gctgaatagg tattatgctc atcgttactt
    25741 ttcaccttta tggtaatttt ctttgttctc aagcgaagga aacgaaagta tgactctttg
    25801 cacggtattg cttgtgaccc ttcttgtggg aataaatgct ggcggtgaaa ttcttttttc
    25861 cgaagaaacg ctgacgctgc ccacttatga aaccatgccg ccggataaac tgcccctgtt
    25921 ttaccggtct gaagaggttc agctggcgga acgccatatt tatccctatc ccttttatga
    25981 tgttcagagc ggcgataaaa aagatattca atataaggcc ctgattctgg aaaacgaata
    26041 tctgcggctt tgcgtgctgc ctgaactggg cgggcgcttt tactccatgc tggataaaac
    26101 aacgggctat gaggcggtat ataaaaatcg agtagtgaag cccgccctga tcggtacgct
    26161 cggcgcatgg atttcaggcg gcgtggaatg gaacacgccc caccatcacc gcgcaaccag
    26221 tatgattccg gttgattatg ctgtcagtaa aaatgaggcg gacggcagcg tcacggtctg
    26281 ggttggcgag tatgaaaaaa gaagccgtac acgctggatg acagggctga cactcactcc
    26341 gggcaaggct tatgtgaagg cgaccttcaa aagcatgaac gtgacgccgc tccaataccc
    26401 cgccctgtat tttgccaatg cggccgtgcc ggtaaatgat gactatcagt ttattttccc
    26461 gcccgatgtg gaattgatga cctttcacta tgtgacggaa ttctgccgtt ggcccgtgct
    26521 gaatcaggtc ttccagtcct atgattatac gcatggtgaa gatctctcct ggtggaacgc
    26581 agccaagcag ccggtctctt tctttgtgat ggaaacccgt cgggatttta tgggcggtat
    26641 cgaccacggc agaggaacgg gcattgtgct catcggcgat catcgaatct tcaagggcaa
    26701 gaaactttgg aactggggca aaaacgaggt gcaggaagtc tgggacgaaa aactgaccga
    26761 tgaggacggc ccctatgccg agctgatgat gggcttttat tccgataacc agccggacta
    26821 caattttgtt gcgccttttg agaccaaata cggggatatg tatttttatg gcatcaaggg
    26881 actgcgggac atcaaagagg caaaccgtga tcttgccgtg aatctcgaga ttaagggcag
    26941 aaaggcgctg attcagatta atgccaccct tgctctgcag gaagtgaaac tgaaactttt
    27001 gggttcggga aagcccctcc tggaacaaac gatccagctg gatccctctg cgctttatga
    27061 aacatctgca gtcctttccc gaaagacgaa gatggaagat ctgcaaatcg tcctgaccgc
    27121 tgctgacgga acggaactgc ttgcctggga gcagccggta aagaaaaatg agcccttccc
    27181 cgaggtctat cacgatcctc tcccgccgga tgaatatacg accagtcagg atgcttattt
    27241 tgcggggctg aaattggaac aattcggcaa caccaacttc gattacatga aatattatga
    27301 acgggcgatt gcgctgaatc ccgcagatgt ggcggcgaat acccgactgg ggctcatata
    27361 tctgaagcgc ggcgaatttg accgggcgga gggttttctt cgcgaggccg tgcatgtggt
    27421 aacggataac cacaagaagg cgaaggatgc cgagtccctg tattatctcg gcctgtgtct
    27481 catgtatcag aacaaagtgg aagaggcgct ggatctgttg taccgttcca cctgggatta
    27541 cacttggacg tctgcaggct ataccctggc ggcgcagctg gaaagcaggc aggggcgttt
    27601 tgagaaaggg ctggaagcgg cggatcgcgc ctggagggcg aatcaggaca atgtcgaagc
    27661 ctgccagtgc atggcgctgc tttaccgccg gttgaaagaa tgcacagaag cgcagaacat
    27721 gcaacagcgc tggcttgagc tggatcccat gagcatactt gcgcgcaatg agcaaattct
    27781 ccttaaaggc gggtacagtg aatgcgatcc ggctcttgta actgatctgc ttaggcttat
    27841 gcgggatgag ccgtataatt ttattgagag tgcaatacgc tacgcgtctt tcggatttta
    27901 tcaggatgca gttgcgattc tacggcttgc ggcggactct gatcttcctg cgctcaacgc
    27961 ctatcctatg atttattacc acctcgcctt ttatcaggat cgggcaggtt gcacagaagc
    28021 cgccgcaggg tcgctggcaa aagccgcggg gctttcctcg actctctgtt tcccctacgg
    28081 gctggagtcg atagaagtgc tgcgttatgc cctttcgaaa aatccggagg acgtgtccgc
    28141 cttgtatctt cttggcaatg ccctggccga ttatcagcat gaggcagcag tgacatgctg
    28201 ggcgaaagcc gcagaactca acagtgccga tccggtttcc ttcaggaata tggcttatat
    28261 tcaggcgcat catttccgtg atatgcagaa ggcgttggat aatatcatgt gcgcgctgag
    28321 cctggatccc tccgagccac gctatttttc ggaagcacat ctgtacatgt cctatgccag
    28381 cctcagtcct gaggagttgg agcagttttt ggaagaatat gccggtacca gcgggcatat
    28441 gactgatatt cagctgatgc agatcaaact gaatattttc cgcggcaact ataccgaagc
    28501 gattgcgttg ctcgagcaga tgcaatatca catcaaggaa ggggcagact tcaatcctca
    28561 tgtgtactgg tttgatgccc atctttacga cggaattgca gcgatggagc gggggagtta
    28621 tgaagaggcg aaagcccggt ttctgcgtgc catggaattt ccgaaaaatc tcgaggcgga
    28681 acggaacggc aaaatcggca tagctcttta ttatctgggg cttaacagcc tggcctccgg
    28741 caatgccgac gaggcgcggg agtacttcac ccaaatgtcc gcatatacat acacgcaggg
    28801 atggggcgcc ggtgatttcc cggagattgc ctactatcag gcgctggcac gccgccagct
    28861 aggagaaaaa gaggatgcgc tgcaggaaat cttcaggaag ctgatcgcag acggtgagag
    28921 caggcttgct gcacgcaccg acagcaggca tatcacggtg tctgttgatg agtcgcattc
    28981 aggtcgtatt ttcctattgg aaaaagaaat tgcccgtaag aatctcaagg tctcttctta
    29041 ctatatgcag ggtcttggtt atctcgggtt gggcgaccgt gaaaaagcga agtccttttt
    29101 caatcaggcg ctgcaggttg atccgctcca tgtcgatgca caaaggatgc tgcgcactct
    29161 gtagtggtat acgtctggag caatcgatac gcgggaaatt ggctgtgccg atacctgaaa
    29221 ggtcggcagg cgcgacctct gtttcaatta cggctgaata aaaggataaa gggctgtccg
    29281 caaattggct gaaaaataat tggctgaaaa gttgacagcc accgccgctt ctgttagaat
    29341 aaaatccccc tgagcgggac taactcagtt ggtagagtgc cagcttccca agctggatgt
    29401 cgcgggttcg aaccccgtgt cccgctccat ccttttcccc acaatcccca atgtttacag
    29461 gggtattcgc tgtgtctgaa atatcgctga ctgcttttta gattccttag gaaaacagaa
    29521 cgataaaaag gggggagcct ttattcaaag actccccgcc gtgttgccaa gaggcattat
    29581 cgtctgttat ttatccagac cgcagtcgcc cagaggaatg cccaaagcat cggctactcc
    29641 tttggcgtac gccggatctg ctttgtagca gttattgatg tgtcgaattt tgatcatgtc
    29701 cggtgcatcg cccatattgc gtgccgtgtt gccgaagagc gcttctttct gttcaggagt
    29761 catcagattg aacagttttg cgggctgcga atagtagtcc gtgtcttccc ggtgatccca
    29821 gtggaaggcg ggtccgttca gatccagcgg cggctcggaa aaatcaggct gttcctgcca
    29881 ttcgccgtaa ctgttgggct catatccgag ggtgcttccg tagttgccgt ccacacgcat
    29941 ggcaccgtcg cggtggaaac tgtggaaagg gcagcggggc tggtttaccg ggatcagatg
    30001 gtggttgacc cccagtctgt agcgctgggc atcgccgtag gaaaagaggc ggccctgcaa
    30061 cattttatcg ggcgaaaagc cgatgcccgg tacaatattc gcaggattaa aagcggcctg
    30121 ttccacgtcg gcaaagtagt tttccgggtt tctgttgagt tccatcacac cgacttcaat
    30181 cagcggataa tctttatgga accacacttt ggtcaggtcg aagggatggt agggacaggt
    30241 tgccgcctct ttttcgggca tgacctgaat gtaaagcgtc cacctgggga aatcgccttt
    30301 ttcgatgctg taataaagat cgcgctgatg actttcacga cattgtccaa tgacggcttc
    30361 cgcttccgca tccgtcaggt ttttgatgcc ctgctgcgtt ttgaaatgaa acttgaccca
    30421 gaacctttca ttcttcgcat tgatgaggct gaaggtgtgg ctgccgaatc cgttcatgtg
    30481 cctataggta gccgggatgc cgcggtcgct catgacgacg gtcacctgat ggagcgcctc
    30541 gggcaggttt gtccagaagt cccagttatt cagtgcactg cgcagattgg tgcgcggatc
    30601 ccgttttacc gcatggttca ggtcggggaa tttcagcggg tcgcgaagga agaaaacggg
    30661 cgtgttgtta ccgacaagat cccagttgcc ttcttctgta tagaatttca cggcaaatcc
    30721 gcggatatcc cgctcggcat cggcagcgcc ccgttctcct gccaccgtgg aaaagcgggt
    30781 gaagagttcc gtttttttcc cgacttcaga aaaaatcttt gcccgggtat actgcgtaat
    30841 gtctccggta acggtaaaga caccgaaagc gcctgagccc ttcgcatgca tgcgccgctc
    30901 gggaatcact tcccgatcaa agtgcgccag tttctcgaga aaccagtgat cctgcagaag
    30961 catgggcccc cggggccctg ctgtccacac attctggttg tccactaccg gcgccccgga
    31021 attggttgtc agtttctttt tgtctgtcat ggttacatcc tttcatcagt tgaaccacat
    31081 cccacaaaca catgtttttt cgttcatgca aaatcaaaag aaggacaatt attttttatc
    31141 tttggctttc agctttttcc ggcaggctgc acaaatccct ttcacctcca gatgcagcga
    31201 ctgcacttcg ccgaatgcac atgcctcttc gggaattttc aacccctgcg ggtcatcggt
    31261 gacaaaatca cggatcatac cgcagcgggt acagctgaag tggtggtggt gcgccatatt
    31321 gccgtcaaag cgaaggctgt catggctggt tccggcaatc tgcagaaagc ctttctccgc
    31381 cagcagcttc aaatttctgt agaccgtatc cagcgaaatg gagggaatgc gttccttcac
    31441 ctgccgatac acggcctgcg catccggatg ggaatccgtt gcgatgacag cccggaaaat
    31501 ctcggtgcgc tggtgggttg cttttgcccc tgcccggcga catgcctcct gaaaagcacg
    31561 tattctttct tcaatatgtg tcgggtctgc tttcattaag aacaattcct aaataagagt
    31621 atatcttaat aagaacgatt tgtcaaccct gaatgaaaaa gaatgaaaaa gaaggctgcc
    31681 cccgtacgca ggaggcaacc gaaaatacag gaggataggc gttaggtgtt aggtgttaga
    31741 gaattgggaa tatgggatag gactgacgat cttgcgccaa ccccctggga acgccaacga
    31801 cggttttata cggctgggat ctcgtccact ctgtccactc tgtccacttt gtccccccac
    31861 tttggtgtgg aatgcagtcc tgtaaaccaa agacgcccat gccctgatta agattcctgc
    31921 cttcgcagga atgacggtgt taggaaaatg cagggatcga cgcggaataa attgtttttt
    31981 gctgtattcc gtgatgctca gttctcatga atagagcagc acgtctagcg gtgcgggttt
    32041 tcttcatcct attgcccagc gtgggaatcg ccaggcgggc gtttttgaaa cagatgaaga
    32101 ctgtctagtc aatgatgaat gaaaaaaggc tgcccccgtg cgccggaggc agccggtcag
    32161 ccgacttatt tcgccggaga atacactttg tcatagaagc gggcaacctg cccctgcagc
    32221 agggcgagtc tatccacagc ggacgtatca ccggaagcat actggatcat gagccttcct
    32281 tcctcaaagc agagttcgtc atgctgaggc agaacttcgc gcaggagata ggtggcatat
    32341 ttgacaatgc ggaagggacc gtacgcgcgg gcgagttcgc ctttgggtgt ttccactgct
    32401 acggaacgga agcctttgat gcctgcaatc aggtcctgca gatcggaaga gggtgcgaac
    32461 accagtgtgt aatagcggcc aaactgatcg ctgcgttcat ggccgtgggt gtctccgatg
    32521 ccgcagacag gcatgcgccg tccctttgca cactcttcaa catagcgtgc gacctggatc
    32581 ccgttgacat cgaggctgtt caaactgttc caatcgaatc cgctgatgac ttcataggca
    32641 tcaaaaatac ccgtggagaa aatatgatcc atgagccgtt catccacatt ataacggttg
    32701 ccggttaccc agtatggatg acagaacatg ctcatgccgt tgaattcggc aatttttctg
    32761 gtttcccaga cacaggcggc ataatggaag ggattgactc ctgcgggcag atccgtgagg
    32821 gcagcctgaa tctccgcgat ttccctgtcg catgcagcct ggtcattttt ccacaactcg
    32881 gtgagcccct gctccgcgcc gaagctcagg atatggacag ggttccccgg gaaatggcat
    32941 tcttctcctg ggtaaagccg caaatcgatg ggcagcccat tgtaaaagtc gcgggcttca
    33001 atggagccac cgcgaaaatg gtggtctgag agggcagtaa agtcaagacc cgcacgacgg
    33061 catatcccgg caacataagg cggggattct accccgtcgg aacggtggct gtgctgatgg
    33121 aaatcacctt tgtaggggcg catgccgtag agatcgggct ttaacgaata aatgcggaat
    33181 ctgcccgcct gagtggtttt tccgtccacg gttttgttca gaataaagtc atgttcctgc
    33241 tcctcttcaa agagcatggt aatggagatg cgcccgttct cggggataat cacagttttg
    33301 gtgccgccga cagggccgcc ctttaccggt tcgaattcca tgggtgtata ggtcagttc
//
LOCUS       DAOPWB010000014        31364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1367_length_31364_cov_4.724808, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000014 DAOPWB010000000
VERSION     DAOPWB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 31364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31364
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_31364_cov_4.724808"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="PKN92_02600"
     CDS             <1..149
                     /locus_tag="PKN92_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNZ47713.1"
                     /translation="VVKDGHAYIQAGAGIVHDSNPDKEYEETVNKAKALMKAIDFAEK
                     GLEP"
     gene            146..709
                     /locus_tag="PKN92_02605"
     CDS             146..709
                     /locus_tag="PKN92_02605"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNZ47714.1"
                     /translation="MIIIIDNYDSFTYNLLQPLAGLKQEMQVVRNDALTVEALKEMKP
                     DKILISSGPGNLKDTGICLEVIREMGEYTPILGISLGCHCIARLFGAELIPADTIMHG
                     KNSDISHGDTALFKGIPSPFSATCYHSLLVDRASLPACLEVIATDAKGSVMGIAHRSF
                     PVFGVQFHPESIQTQHGGLLLRNFVGL"
     gene            851..>3170
                     /locus_tag="PKN92_02610"
     CDS             851..>3170
                     /locus_tag="PKN92_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47715.1"
                     /translation="MKAFTHSLLTFLIVTIAFAASYGADDFVVHGRVSFDTGGFMVKN
                     ASETDWALAAVNTMVLPGDTVWVDEEGTAEIELPNSSFLRLSDGSKAEITALPPNALL
                     RGWVGSFYVHRLSRSAGAFTLTTPSATTTIAPDSMVRIDVNAEGGTAVSVRWGSATVQ
                     APQGDAVTATESNRVWVDPGYLPSNPTPFDRNVTDAFDNWNNDRARLLAEGAKTIPRE
                     IYSETAPSVGSYDLARYGDWVMVDNRSYWRPTVVVDYVPYHYGCWNYMPAVGHVWVDE
                     YPFGYITSHYGRWRYTASYGWLWSYDPVWSPAWVASVHVSNYHVWAPVDYYNRPVFVT
                     GGTFFTIGGVSFCSYGTSYVSSSYLYSGPGYIGVPNTTIINVFNTTPSREINIWNLSP
                     HAHRRPAIPWQGGILAQERDYNPRRSIRGFSGNTELTLASRDRVERLERALNRDSFAP
                     RRETAARQSERTTLQNRSVAAMPRSVRMNQSEQTYRPRGEQAGGRGDTARSLVPESAR
                     IGRTPFSPGDRQRDSNTMEARSTTAAPQRGTVNTDSMRQPRSAAGRQKGTAGTQGTRG
                     GIRTNPTNVAVRDLDTAAPAPRGSGQLQRTPVRESQPVPRSGRGAAENPRNTPAPPAA
                     TTGRQAPEARPIPTGTRTAPAPTPPTRSMAPSASTAPSTRPAPTHTVPAPSVRTAPAP
                     SVRTAPAPAPSVRTAPAPSVRTAPAPAPSVRTAPAPSVRTAPAPAPSVRTAPAPAPSI
                     RTAPAPSVRSMPAPSVRTAPAPAPSVRSMPAPS"
     assembly_gap    3171..3270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3271..3535)
                     /locus_tag="PKN92_02615"
     CDS             complement(<3271..3535)
                     /locus_tag="PKN92_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47716.1"
                     /translation="MYLQRPRRTEPPEGAERTDGVENPLGALGMERVLIDGEEKPRGA
                     LGAGAERTDGAGAILGALGAGMERTEGALGAGAERTDGAGAILG"
     gene            3568..4479
                     /locus_tag="PKN92_02620"
     CDS             3568..4479
                     /locus_tag="PKN92_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47717.1"
                     /translation="MKNLKTLSFPVMALLLAVALSGCPSGKESEYNLGFNVGFLNDAK
                     YWEGFDDSYETVPDGPIFYAGASIPELTDGSYDAGYWDGIWYAYNDGYFVCYDYAFMI
                     GFSEGYDAAYQKNWKSFLLQDSHPEYLDGSFEDGYNDGFTEGSVFGADDYKRKAPFNW
                     QDALWDYRDNIDVYLDELGFGTGEWGDVFLYEYGTDPHEFYKTKNAAASGTATRLLFQ
                     KNNGGRGLRGTALQAKAAVNAAAPKAEELEIPALSYRSLPGRIQQSLPTRTLYVDRRQ
                     GTESHLNTTWLQRINAYRDAIGKNSGE"
     gene            4667..6013
                     /locus_tag="PKN92_02625"
     CDS             4667..6013
                     /locus_tag="PKN92_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47718.1"
                     /translation="MQQRRLLVLDALRGFFILFVICIHAVSGVIYGNNAQALENVPLW
                     QIILFAPLLLISTWAPIFVVISGMAQAYVLSGLILSKKAANALSLRNFFGGVLVTSLF
                     LYLLSLVNMAFFHHPMDYAGEFRYTFLTGALHAGHWVPFDMRLLFYNDALSMISINGL
                     LVTCILYFLWKINWIPRTRKASLLLLGVAASFFALSPLLHQKLDPIFLTAVENGHAGT
                     AFFLRFFVGIGFSTFPYAAYGLTGAILGLFLAKRKEEIWLRRDGYRAATAMVLSSVVL
                     MGITGFAPKDVIHHPYPFKMQLLVLGTILLTCIWLILKLEYFTEEQRAYRAKKTVWLR
                     RFGLLALTIFCFESVLSMLFSKAYCFLWGFDEAFPKTAPHILFFLALNVLFWTFVLKF
                     WEKINFKYSIEWWISCLAGIVRGRPSARLQADAVLYKPCLLPEPVASPCPAGLENR"
     gene            complement(6045..7379)
                     /locus_tag="PKN92_02630"
     CDS             complement(6045..7379)
                     /locus_tag="PKN92_02630"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNZ47719.1"
                     /translation="MQNDLSDGGTGLVAEPSDALAAREYLDSLILHGIKLGLSNIEAI
                     LREGGEPQYSCPVVHVGGTNGKGSVLAFLDAMLLAAGYRTGRFSSPHLLDVTERFLID
                     AVPVCNATLEESAHWCQLMSARALCVPTYFEANTAMAFYCFQKKKVDVALLEVGMGGR
                     FDSTNVVQPRICAITNIALDHTRYLGSTLAEIAFEKAGILKKNIPAVLGRIDPEAQRV
                     IEQEAAARAVPLFRWGIEYEAEKGGSPLTPVLTYRGNGKVFENVQLGLAGMHQIENAA
                     IALTVALQLQSDFPRLTDSAIISGLEKAVWPGRLERLLDSPPVLMDVAHNPAGCAALV
                     EAVDNCTAVFAVSSDKDAATMLALLKRIARPLILTCYTGERSLPLTALRQFADSAQCI
                     IVPSLAEALEAGKREASPEKPLLITGSIYACGEARDYMIRNWGVSAPAFRRS"
     gene            complement(7385..8116)
                     /locus_tag="PKN92_02635"
     CDS             complement(7385..8116)
                     /locus_tag="PKN92_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNZ47720.1"
                     /translation="MINLLKLQRLDTNIADCIQKEKELPRQKDKFFIQKERLAEEIKN
                     SEEHCRKIQAEQRECEAGIEQLQEKIKKYEGQLPDIKRNEEYKAVLHEIEMQKKQISN
                     KEERVLVLMDAHEEALAKLEEDKTRVQGEISKLDAECAAIDADLAELVQHRKALQAKR
                     PELKEEVEPQLLSLYERIRKVRVPAVVPLNAESCGGCFMTIRPQIVNEIMGSEKVHTC
                     QHCHRILYYPDNVSLSGAETIEESA"
     gene            8604..9410
                     /locus_tag="PKN92_02640"
     CDS             8604..9410
                     /locus_tag="PKN92_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ47721.1"
                     /translation="MVPLLTRRDFLKISAAGIAAAALPVPSVLGEPRKTGSFTWAGIN
                     DLHIKNRDCLPYISRVVAALNDNPAVSGVVVLGDLATMAQPEELRLAKEALKPLNKPC
                     YILPGNHDIGPETEGALKRFNDIIGPEHWLLEWEHWTFLGFNSCVGAKSDVILPESEI
                     NWLRDQLISIDSERPLALFCHHPLNPHSKAYRIINGDEILDLFSHHALRLVSSGHWHG
                     NQEESTAQHLFTTTACCSSTRDNFDKTAAKGYRLFHFGEDRADTEFVEVR"
     gene            9701..10117
                     /locus_tag="PKN92_02645"
     CDS             9701..10117
                     /locus_tag="PKN92_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47722.1"
                     /translation="MTIFLNFQRKLFAGILLLTVPAALLFWQLSHPFTEAYLIGLAAA
                     LISLLLKFRLAHMTLKRLTYTLPWHSLCSMSLYMMALYGGYKIHAKHLLGLAGVSIAL
                     GLVLLVLFFLGLTELDLLGTGHHSSVFRTGHRKDEK"
     gene            10117..10878
                     /locus_tag="PKN92_02650"
     CDS             10117..10878
                     /locus_tag="PKN92_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HNZ47723.1"
                     /translation="MEDLGHRLVWHYIPFTNLEIPFGGVNILTFANTAFVFVVALGLL
                     WLGARKRETIPGRGQMLFEFLIQTFRDMLEPSVKQEHLRIARRSLPVIMSLFYFILVS
                     NAIVLLPIPHVEEPTGDLNCTFALAVVAVTYAILSGIRQSGLKGTFGEFCGPFWHQEN
                     QSGWGFYAGKLSALFFFPLRILEEISRMISLSCRLFGNIFGVAIVIAVVSSITYYVMV
                     PLALYGVLVVFEAGLQAFVFATLSVTYLSSAVNPH"
     gene            10899..11222
                     /locus_tag="PKN92_02655"
     CDS             10899..11222
                     /locus_tag="PKN92_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNZ47724.1"
                     /translation="MNGSIFVTFMQSVAAQAEAAQAAQGVSGMDLRVIFACISAALVM
                     SLAALATGLSEGLATGKAVESIGRNPESAGGVTRALVIGLAITEAVAIYALLIAAMIL
                     FMVID"
     gene            11215..11676
                     /locus_tag="PKN92_02660"
     CDS             11215..11676
                     /locus_tag="PKN92_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47725.1"
                     /translation="MTDPLFLSARMLTLNFTSVVALAVFLIFLRIASRIFWRPMIRHM
                     VRRERLFAVQEESIQQQLSDAKALQERIQQRLDQKKKILSHDVDLKLQQAHSSRRLML
                     ASEKNKTEQHLVQLHDQMQEYLETERRRFAEALPRLIEEMDLRFQQGGPHL"
     gene            11673..12431
                     /locus_tag="PKN92_02665"
     CDS             11673..12431
                     /locus_tag="PKN92_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47726.1"
                     /translation="MKNQRIFFAFFCIAYALLSATGIFLFGRPGYSKEYLANNHEDHK
                     RYLAISKNPLYQKYCERPLLNPLDQHLQKEADFAAAYTARPAFRAERMRMFLYAIWFK
                     VLNALFLFILFVRFGLPIARTFLDSHIHQIQTKKDTLEDELARASSQAAESREAFSHL
                     PNQEAALEQSFDDLYKKKLADIEKQSQHALEQLAIDTEKRIAAEEQAAAAAVRRELVD
                     NALHELERKYRKEPSQEHLIKSVEQFCQYMEIIS"
     gene            12428..12991
                     /gene="atpH"
                     /locus_tag="PKN92_02670"
     CDS             12428..12991
                     /gene="atpH"
                     /locus_tag="PKN92_02670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNZ47727.1"
                     /translation="MRPEQIAVKYTLALRATLHTEEDLHRTVATYRQFMTICGKEPDL
                     LRTLSNSIVPLDHRKQLLETALSICNPPPSLTALAFLLLERNRFSLLPVIARQFEQQM
                     DQWLNRVEVEVITAIPLSAELFQKVHHTMELFTKKTVRLVQSIDPDIIGGLIIKMEGL
                     AMDFSYRARLEKLREKLLSEGRKIHGA"
     gene            12981..14516
                     /gene="atpA"
                     /locus_tag="PKN92_02675"
     CDS             12981..14516
                     /gene="atpA"
                     /locus_tag="PKN92_02675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNZ47728.1"
                     /translation="MALNPRTDEIVEIIRRQISDFIWSAETAEVGTVIQSGDGIARIF
                     GLDDVAAGEMLAFPHGVKGMAYNLESDHVGAILFGDYHKVREGDTVKRTENITSVPVG
                     ESLIGRVVNALGEPVDGSGPLTNTTDLPVERLAPGVIDRQPVNKPLQTGIKAIDSMTP
                     IGKGQRELIIGDRQTGKTAIALDTMINQRGKDVICIYVAIGQKQSTVARLMQELADHG
                     AMKHSIIVTASASEPAPMLYLAPYTGCTIGEYFRDSGRDALVIYDDLSKHAVAYRQLS
                     LLLRRPPGREAYPGDVFYLHSRLLERAAALSEEKGGGTLSALPIIETQAGDVSAYVPT
                     NVISITDGQIYLESSLFHAGIRPAINPGISVSRVGGAAQADAMKKVSGPLRLNLAQFR
                     ELQAFAQFGSELDKTAQAQLERGERLVEILKQDQSKPLSVSAQILIIRCASIGLLDDI
                     AVRELDRFEEELFAFFNLNHEALLEELASTRHFDEALKQKLDESLKHFKEQFFCGESR
                     TDG"
     gene            14509..15369
                     /gene="atpG"
                     /locus_tag="PKN92_02680"
     CDS             14509..15369
                     /gene="atpG"
                     /locus_tag="PKN92_02680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNZ47729.1"
                     /translation="MANLQEIRRRIGSVKNTQKITRAMKLVAASKVRHAQKALATSRT
                     YAAHVYSLAEEMLFESQDYAHPLLEERGGKSACLLVVTSDRGLCGSFNHSLFQEAAQW
                     WQTHRRDKEVFIHVIGKKGMDLFQRSRIPVQEYSTGVLEGNPVHTAIDLVNSYIRGFS
                     AHLFDEVYCLYSRFATVVSQEICLEKMLPCRFEKTDTSAFSDWIIEPDRRALLSRLIT
                     SNLVAQQYRILQESLASEQGARMTAMESATNNAGDVLASLTLSYNRLRQDAITREVVE
                     VISGASSQEF"
     gene            15378..16781
                     /gene="atpD"
                     /locus_tag="PKN92_02685"
     CDS             15378..16781
                     /gene="atpD"
                     /locus_tag="PKN92_02685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNZ47730.1"
                     /translation="MKTGKITQIIGPVIDVQFEAGNLPPINTALEVRPPKSSVVTLEV
                     ALHLGDNVVRTIAMQPTEGLLRRMEVVNLEQPISVPVGRETLGRILNVIGAPVDGLGA
                     VNTKERWPIHRSAPDLKDINIRTEIFETGIKVIDLLAPYPRGGKIGLFGGAGVGKTVL
                     IMELIHNVAINHGGYSVFAGVGERTREGNDLWREMKQSGVLEKAALIFGQMTEPPGAR
                     ARVALTGLTVAEYFRDVAGQDVLLFIDNIFGFTQAGAEVSTLLGRMPSAVGYQPTLST
                     EMGELQERITSTRKGSITSVQAVYVPADDLTDPAPATTFSHLDATTVLSRKIVELGIY
                     PAVDPLDSTSRILDPRYVGEEHYRIARDVQKVLQRYQDLQDIIAILGMDELSEDDKAI
                     VGRARRIQRFLSQPNFVAEEFTGIPGKSVPLEETLHSFKLILAGDYDHYPEAAFLYCG
                     GIEDVVEKAKTLGSHAS"
     gene            16778..17212
                     /gene="atpC"
                     /locus_tag="PKN92_02690"
     CDS             16778..17212
                     /gene="atpC"
                     /locus_tag="PKN92_02690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNZ47731.1"
                     /translation="MIQTGSAKRLLQVEICSMDKAPVRLEASLVKLEGGLGPFTVLPG
                     HAPMLASLDIGVMTLHSADGERFFYAVNGGVVKVCNDQVLILPQSFEESGEIDMERAQ
                     QAKTKAETILKERHSSELERMEVVLKRAIARIRARSYSEGKV"
     gene            17348..19816
                     /gene="priA"
                     /locus_tag="PKN92_02695"
     CDS             17348..19816
                     /gene="priA"
                     /locus_tag="PKN92_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNZ47732.1"
                     /translation="MDLFSSTSPAVKVALPLSLDKVLTWLVPPELVDKARPGMRALVP
                     IQSRIVLGIIVEVLAESDVQEARTLIDIPDHEPVLDKNLLRLCKWIADYYCCSWGEAL
                     FSALPSSLKAVPKKQYYLNENKLDSGRLSEMQRKIVGALYRQSPQTVVQLSKSVGTGA
                     LQSALRSLLSRKIIFEDHLFQDRARSILTETRVSLAADAPGDPETLQALQKKAPKQAA
                     LYLELLYGEKEQSADLLCRKHNTTKSTFKSLEKRGWVHLQETEVYRRPSLPAGFHAKD
                     KFKLNEEQLNAYNAMCTPLLKREYHCFLLHGITGSGKTEVYLQIIEKALSVGRTALML
                     VPEISLTPQTVGRFYARFNQDIAVFHSGLSEGERYDAWRRTREGKVRIVVGARSAIFA
                     PLQNIAVIVVDEEHDHSYKQGEAPRYHGRDVAIVRARMENALCVLGSATPSVESFYNS
                     EQKKSSRLVLLNRATTSSLPEVRIIDMRREVAETPGQLILSTELEKAVQKRVAANEQV
                     ILLLNRRGFAPVVLCPVCGWIPECDNCQVSLTYHRAGGNLLCHYCNTSRSNPLVCSQC
                     GFNPLLYLGAGTQKAEDYLVRSFSGARVERMDGDTTSGKGGHANILERFARREIDILI
                     GTQMLAKGHDYPGVTLVGVINADAGLTIPDFRAAEQSFQLLTQVAGRAGRGSLGGEVI
                     IQTHRPQHYAVQSASRHDYAAFYAHEIQQREAAGYPPFRRLVNFLIEGEDPLYTEKAS
                     LLLRRLALAQADEMGFHGIAFLGPAPASVRRVKKIYRWNFALMSRSAARVNALSRALR
                     EVFENNAPEGNIRLKIDLDPYGMF"
     gene            complement(19891..19966)
                     /locus_tag="PKN92_02700"
     tRNA            complement(19891..19966)
                     /locus_tag="PKN92_02700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19931..19933),aa:Met,seq:cat)
     gene            complement(20090..21217)
                     /locus_tag="PKN92_02705"
     CDS             complement(20090..21217)
                     /locus_tag="PKN92_02705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNZ47733.1"
                     /translation="MSFSLIAAVSIYIASQTVQQGYEQLPPSYICIEAETGKIITESN
                     ADLQRPPASMLKMMVMLLADEGVHAGKWSYDTPITTSKAAQSMGGTQIYLAAGDIFPL
                     EDMLMAMAVLSANDASVAVAESLWGSVPACLDAMNRRAEELGMKNTHFTSVHGLPPAD
                     RENFDLTSARDMAILAKESLKYPQTLVRSSTREFALRPNTPPRPSTNKLMDRMPDCDG
                     LKTGYIRASGFCLTATAKRNDIRLIAVLMGSDREGRFNHTRDILNECFAKITRVQPVF
                     AGMSIGKAIPVQKSMEEEVGLRAASDITALINKDESDRLFLEITAPVSLEAPVLEGTE
                     CGNVRLMLGDEILGTSALYTDRSVERVRLIDRGLGCRGKRN"
     gene            complement(21236..24046)
                     /locus_tag="PKN92_02710"
     CDS             complement(21236..24046)
                     /locus_tag="PKN92_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ47734.1"
                     /translation="MNLTTVFKRRLTYYIVPVLIGLGGVAFSQQLDNMALRAFVLFLS
                     VLVPLYTGSSLLVRYYTSPMERFGMALGFLLLVTGASVVFSGVFDRLSEVEYVADSIN
                     VLARYIGIISLSVGFVVVFMSIMRKGAGIEELAERFRFLAEHISDGFILSTPDGTVLL
                     VNQKALDIFGKSRQSVIGHNARELALSFGFETIARQLDNRAVGVASEYEINWTVNGEA
                     RILLLNGAPVFDKRGNHSLTMATIRDVTEHRQLTSRVEQYARGLQQLVDEQTRKLHRS
                     EEQLRQLLLSMNEGFLTVDMQYRIRFANEQAEKLLNASTESLISREIFEFVDSVGRSR
                     LMNLFSKSIEHKPGHALRQEIELLKGGKPETSVLVGVAYLPSEEEKDNGFSLVLTPID
                     DLKRMQIQLVARARDLERANEGLRLHDRAKDSFLSNVTHELRTPLTTIQGYIEMLKED
                     DMGTVSDEQRRALVIMDRNARHLLNHINELIEFSRMQIRGVQIASNLYDASALAREAM
                     TSLVPAAEEKGVSLAVEVPDNAIYTWGDRDKISQVLGILLNNALKFTDKGGKVTVRAA
                     MKTHRTVSFEVADTGIGIDQKYHEKIFAKFFQVDSSKTRQYEGAGIGLSIALNIVQAH
                     NGSISVQSAKGKGTTFTVHFPNSTYLSGVKEEMIPLLGALRILLVDESPERQAAFKTM
                     SPLRHCRLTCTSNAYEVTRWEDPDDFDIIIINDAQADVAGSTSRRILRQQFSLAQLPV
                     LVLTNEDVEKITTDLDADSQVFFFYKPCTAQALADKILYILTGDEVYKKELEDPSALV
                     DPDKLQAVVVDQDAEFLEWMNKALHLQNIEARVATAPERLLELVGQKKTDAVFLDADI
                     PTGQLAALLNSYFISSETRRVPLFLISGMRNTVDAAAKLDGIAGVLHKPFPISDMTAL
                     IKKQRDIQQEKSRLKNEAFHKS"
     gene            complement(24081..24545)
                     /gene="rnhA"
                     /locus_tag="PKN92_02715"
     CDS             complement(24081..24545)
                     /gene="rnhA"
                     /locus_tag="PKN92_02715"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNZ47735.1"
                     /translation="MTKPQVIIYTDGGCEPNPGVGGWGAILLYGERRKEIYGGQPHST
                     NNRMELSAAIAALSSLKRPCKVVLYSDSNYLVKGMQEWLAGWKRNNWKRKNEPVLNRD
                     LWEELDAVSASHEIEWVWVRGHAGNELNEACDRLAAKAIEEQRASANYEAPR"
     gene            24867..26384
                     /locus_tag="PKN92_02720"
     CDS             24867..26384
                     /locus_tag="PKN92_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47736.1"
                     /translation="MTLVRLVYLPGEPAINSLCPKSALLEGVGQLCEAGFQTDLQHLG
                     EVRSHGEALISETPRVALTPAEAVSGLPAAALTVFYAESRGDYEQVRKASLLLAKYHP
                     ETTQLLAGPFAVHFATCALADLPVLDGAISDHLSATLPQAALYLLGGQKRPDSGDLSF
                     QENGGKAVWKRRAAFTTLSPFVSRRRCEKNNLLQYYPLSFSEGPCAFFYQDKKCSQRG
                     SKSAALLFNEIKWLHESHDATVFHIDIQHVASEEVEELAQRLLDHNLMILYSLGNATQ
                     SFSRTTARLLLASGCRSIGFRIPTGSQRLLEDFYGMQVSISAMQSVLRLCRDTGLQTV
                     LHLTGPCPIDDIHSTAETVRFLEDAHPHSVTLSPLAVVPHSLWFCQPRRFGLSFSYKH
                     YQKQMDPFRLPLTLAGKWNRYRLAFVNKVIQDYAFKAGLLFNADERSMLLLRLLGIEG
                     VSDSSRFLQHLSRLLESNDAAGLSDVIQKFNHRATVAVSRDAAAMPHFGQVAISR"
     gene            26527..26847
                     /gene="trxA"
                     /locus_tag="PKN92_02725"
     CDS             26527..26847
                     /gene="trxA"
                     /locus_tag="PKN92_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNZ47737.1"
                     /translation="MSNVAELTEATFKDAVNSGVSLIDFWAEWCGPCRMMGPILDDVA
                     KAVGDTANVYKVNVDNAQNLAATLNISSIPALIIFKDGEEVKRFVGVTSKGDLISALE
                     AAAE"
     gene            26995..28002
                     /locus_tag="PKN92_02730"
     CDS             26995..28002
                     /locus_tag="PKN92_02730"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNZ47738.1"
                     /translation="MSTKVVAVVGATGVVGRQMLETLEARNFPLSRLVPLASERSRGK
                     TVSFKGETIPVEVLSENSFEGIDIALFSAGGGTSKKYAPLAAASGAVVIDNSSAFRMD
                     PDVPLVVPEVNAHALKNHKGIIANPNCSTTQMVVVLKPLHDAARIRRIVVSTYQAVSG
                     AGIAAITELQEQSRALLAGEPYAPKVFPHQIAFNCLPQIPQSDAFLDNGYTSEEMKMV
                     HETRKILGDDSIAVTATTVRVPVLNGHSESVNVQTEKKLTAAEARALLSKAPGVVVQD
                     DPQHQIYPLAVNSTGKFDTFVGRIRDDISHESALDMWIVSDNLLKGAALNTVQIAEEL
                     L"
     gene            complement(28061..28795)
                     /gene="ubiE"
                     /locus_tag="PKN92_02735"
     CDS             complement(28061..28795)
                     /gene="ubiE"
                     /locus_tag="PKN92_02735"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNZ47739.1"
                     /translation="MKPEPPVKPDMIHAQRNQTRFMFDAIARRYDVINRIFSFRRDVA
                     WRKAMLRTVFKDENHVILDIATGTGDVLKGLAAEAGEAAVFGADISFNMLQAAQNKLQ
                     HGGRTQAVLMQSDAAALCFADRSVDTITIAFGIRNFALLSESFREIYRVLRPGGKVVI
                     LEFSLPENYLIRSAYLFYFRYLMTPVAGKLSRQKDAYRYLNRSVEAFPSPSVIRDHLY
                     NAGFEVVQSRPMTFGVVTLYTASRSE"
     gene            complement(28785..29285)
                     /gene="rimI"
                     /locus_tag="PKN92_02740"
     CDS             complement(28785..29285)
                     /gene="rimI"
                     /locus_tag="PKN92_02740"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNZ47740.1"
                     /translation="MNEFPGIALTFHPLDFYFLPFVEEAEKEAYPEAWNTSELLQEIY
                     NIRSHFFVMLRYGELIGYAGFWDLVDEAHVTRVTVLPPYRRQGFARPLMKNLIKEVSA
                     SGLEAIRLEVRETNYRAILLYLTLGFRPVRYRNGYYRRTGENALEMFKRLEPHEISVK
                     KGMDEA"
     gene            complement(29282..>29799)
                     /gene="tsaB"
                     /locus_tag="PKN92_02745"
     CDS             complement(29282..>29799)
                     /gene="tsaB"
                     /locus_tag="PKN92_02745"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNZ47741.1"
                     /translation="LRVGLSFWKGLALALQLPLVSVPTLDALARMNAFPQGTLVPLLD
                     ARMKEVFGAVYRFQKGCREKIVPDQAASVDFFIEAALSATAPLVFLGDGAQRYASEIR
                     SRAPEAMIAEVHCGLPRADAVAAEALDLWLTQTEHNPAYAVPLYHRLSQAEQAKEAAG
                     TKAATPEKHRP"
     assembly_gap    29800..29809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29810..30066)
                     /locus_tag="PKN92_02750"
     CDS             complement(<29810..30066)
                     /locus_tag="PKN92_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47742.1"
                     /translation="MAHTCSNTETFPLILAGDTSTGYCTVALCQCDKNKPSCIPISTM
                     GLDSRHLHSERLLEAVEGVLEKAGTELQSLSCLAVTTGPGS"
     gene            complement(30071..30388)
                     /locus_tag="PKN92_02755"
     CDS             complement(30071..30388)
                     /locus_tag="PKN92_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ47743.1"
                     /translation="MTTTKKDIAQQMAERLELQPEAARKSTETILSILANALLEGEYW
                     EIRNFGVFRTKQRAPRLGRNIHTGEKVAVPSYRDVVFKPGREMLQRAASPAEFTRPKT
                     RKP"
     gene            complement(30451..>31364)
                     /locus_tag="PKN92_02760"
     CDS             complement(30451..>31364)
                     /locus_tag="PKN92_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47744.1"
                     /translation="DTSYCLHYAKEVLGLKPLAVHFDNGWTSEKARSNMENICAKLGV
                     DLHIVTMDWDDSRELTNANIRACVPYVDLTNDIGIAYTLYSTAAKNDIKYILLSHSFR
                     EEGVAPLLWNYMDARYTRAIIKRFVKTKINHFKNADIHHFLYWLFIKRIKVVNVTNYY
                     NDMGRQIEKTLIERYGWQEPDGHHMDNELFILIYNYAWRKFGIDWRVVDVAAQVRTGI
                     LTREEGLAIMASPPYFDNEEFVSQSLEKQQMSREEFEAIIAAPNKYFLDYPNYYSWVK
                     LFKWPFKLLCRMHILPIHAYEKYFEAI"
BASE COUNT         7502 a         8148 c         7554 g         8050 t
ORIGIN      
        1 tggttgttaa agacggacat gcctatatcc aggctggagc gggaattgtt catgacagca
       61 atcccgacaa agaatatgaa gaaaccgtaa acaaagccaa agcacttatg aaagcgattg
      121 actttgctga aaaagggctc gagccatgat cataattatc gataactatg attctttcac
      181 ctacaatctc ctgcagcctc ttgccggatt gaaacaggaa atgcaggtgg tgcgcaatga
      241 tgcgcttacg gtagaagccc tgaaagaaat gaagccggat aagatcctta tctcttccgg
      301 tccgggaaac ctgaaagata caggcatctg cctggaagtc atccgtgaaa tgggagaata
      361 tacacccata ttgggcatat ctctgggctg ccactgcatc gcccggctat ttggcgcaga
      421 actgattccg gcggatacga ttatgcacgg taaaaacagc gatatttccc acggcgacac
      481 cgctttattt aagggaattc cttccccctt ttcagcgaca tgctaccatt cactgcttgt
      541 tgaccgggcc tctcttcctg cctgccttga ggtcattgcc acagacgcaa aagggtctgt
      601 tatgggaata gcccatcgct ccttccctgt tttcggtgtg cagtttcacc ctgaaagcat
      661 tcaaacgcag catggcggtc tccttctccg gaactttgtg ggcctttagc attttttcaa
      721 tcttgcccga acagaccctt ttgctctttt caaatgctgt taattaatga atgaaagttg
      781 ctgcccgtcg tccagttgta tgaaagcaag tttattcatc ctcacagcaa tttgaaagga
      841 gggccccagc atgaaggctt tcacccattc tttattaact tttttgattg tgacgattgc
      901 ctttgccgcc agctacggtg cggacgactt tgttgttcac ggtcgtgtca gttttgatac
      961 cggcggtttt atggttaaaa atgcctctga aaccgactgg gcccttgcgg ctgtgaatac
     1021 tatggttctt ccgggcgaca ccgtttgggt tgatgaagaa ggaactgcag aaattgaatt
     1081 acccaattcc tcttttctgc gcctgtcgga cggaagtaaa gcggaaatca ctgcgctccc
     1141 gcccaatgcc ctgctgagag gatgggtcgg ctccttttat gttcatcgcc tttcccgcag
     1201 tgcgggagct ttcacgctga ccactccctc cgccactaca accattgctc cggattccat
     1261 ggtgcgcatc gatgtcaatg cggagggcgg cacagccgtg tctgtccgct ggggcagcgc
     1321 cacggtccag gcgccgcagg gcgatgctgt cactgccacc gaatcgaacc gcgtctgggt
     1381 agatcccggc taccttccct ctaatcccac gccatttgat cgtaatgtca cagacgcttt
     1441 tgacaactgg aataatgacc gcgcccggct gcttgctgaa ggtgcaaaga ccattccccg
     1501 cgagatttac tctgaaacag cgcccagcgt gggttcctat gatctggcac gttacggcga
     1561 ttgggtgatg gtggacaaca ggagctactg gcggcctacg gttgtggtcg actacgttcc
     1621 ttaccactac ggctgctgga actacatgcc cgctgttggt catgtatggg tggatgagta
     1681 tccgttcggg tacataacaa gccactacgg cagatggcgg tacaccgcca gttacggttg
     1741 gctctggtca tatgacccgg tgtggagccc cgcctgggtt gcctccgttc acgtttcaaa
     1801 ctatcacgtc tgggcgcccg tcgattatta caaccggcct gtctttgtta ccggcggcac
     1861 cttttttacc attggcggcg tttcattctg cagctacggc accagttatg tatcttcctc
     1921 ctacctgtat tcagggccgg gttatatcgg agtccccaac acgactatca tcaatgtgtt
     1981 caacacgaca ccatcccggg agattaatat ctggaatctg agtcctcatg cgcaccgccg
     2041 cccggccata ccctggcagg gaggtattct tgcgcaggaa cgtgattata atcccaggcg
     2101 ctccattcgc ggttttagtg gaaacacgga gcttacgctt gcctctcgtg accgtgtcga
     2161 acgcttggaa agagcactca accgcgattc cttcgctcca cgacgtgaaa cagctgcccg
     2221 ccaatccgag cgcactacgc tccagaatcg ttcagttgcg gcaatgccca gaagcgtccg
     2281 gatgaaccag agcgagcaaa cctatcgccc caggggtgaa caggcaggag gccggggaga
     2341 cactgcccgc tctcttgttc ctgaatcagc gcgtatcggc cgtactcctt tcagccccgg
     2401 cgacagacag cgcgattcga atacaatgga ggcacgcagt accactgctg caccgcagcg
     2461 tggcactgtg aatacggatt caatgaggca gcccagaagc gctgcaggcc gccagaaagg
     2521 aactgcgggc acacaaggta ccagaggcgg gataagaaca aatcccacca atgttgccgt
     2581 gcgtgacctt gatacggcag cgcctgcgcc cagaggaagc ggccaactgc agcgtacccc
     2641 tgtgagagaa agccaaccag tcccccgaag cggacgtggc gctgcggaaa atccgcgcaa
     2701 tacgccggcg cctcctgcgg caactacggg cagacaggct ccggaagcac gcccgattcc
     2761 cacaggcacc agaacagcac ccgccccgac tccgccaacc cgctctatgg caccttctgc
     2821 atcgactgcg ccgtctacac gtcctgcgcc aactcataca gtgcccgctc cctcagttcg
     2881 caccgctccg gcgccttcag tccgtacagc tccggctccg gcgccctcgg ttcgcaccgc
     2941 tccggcgcct tcagtccgta cagctccggc tccggcgccc tcggttcgca ccgctccggc
     3001 gccttcagtc cgtacagctc cggctccggc accttcggtt cgcacagctc cggctccggc
     3061 accttcgatt cgcacagctc cagcgccttc agtgcgttcc atgcctgcac cttcagtgcg
     3121 cacagctcca gctccggcgc cttcagtacg ttccatgcct gcacccagcg nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcccagaat tgcacctgca ccatccgtac
     3301 gttctgcacc agcgcccagt gcgccttcag tacgttccat gcctgcaccc agcgcgccca
     3361 gaattgcacc tgcaccatcc gtacgttctg caccagcgcc cagtgcgccc cgtggtttct
     3421 cttctccgtc tatcagtacc cgctccatgc caagcgcccc cagcggattt tcgacaccat
     3481 ccgtccgctc tgctccttcc gggggctctg ttcggcgcgg acgttgaagg tacacacatt
     3541 taaactcaac aggcaggatc aaataatatg aaaaatttaa aaacactgtc ttttcctgtt
     3601 atggctctcc ttctggctgt ggcactgtcg ggctgtcctt caggcaaaga atctgaatac
     3661 aatctggggt tcaatgtcgg ctttttgaat gacgccaagt actgggaagg ctttgacgac
     3721 agttacgaaa cggtccctga cgggcctatc ttttatgctg gtgcctctat ccctgagcta
     3781 acagacggca gttacgacgc cggatactgg gacggcatct ggtacgccta taatgacggt
     3841 tatttcgtct gttacgatta tgcttttatg atcgggttca gtgaaggcta tgatgccgcc
     3901 tatcagaaaa actggaaatc ctttcttctg caagacagtc atcctgaata tttggacgga
     3961 agttttgaag acggatacaa cgatggattc acagaaggaa gcgtttttgg cgcggacgac
     4021 tataaaagaa aagcgccctt taactggcag gacgctcttt gggattatcg ggacaacata
     4081 gatgtgtatc tggatgaact tggcttcggt accggagaat ggggtgatgt atttctgtac
     4141 gagtatggaa ctgaccccca tgagttttac aaaacgaaaa acgccgcggc ctccgggact
     4201 gccacgcgcc ttctttttca gaaaaacaat ggcggacgag ggctccgcgg aacagcactg
     4261 caggctaaag cagcggtgaa tgcagccgca ccaaaagcgg aagaactgga gatacccgct
     4321 ctttcctacc gatccctgcc cggccggatc cagcagtctc tgccaacccg tactctttat
     4381 gttgaccgcc gccagggcac ggagtctcat ttaaacacaa cctggttgca gcgtatcaat
     4441 gcttatagag atgcaatcgg gaaaaacagc ggagagtgaa acatttttac ccgttcagtt
     4501 gtattccaat taacaacggt tattatttct tcaactgaac agggcaccac ctgcctgatt
     4561 ttatagagcc ctccgggcat gaactgatgc ccggaggggt tttctcaaaa gtgcttacac
     4621 actgaagatt caggcataat tttgacccgc taccaggata gattccgtgc aacaacgacg
     4681 cttacttgtt ttagatgctc tccgaggttt tttcatcctt tttgtcatat gcattcacgc
     4741 cgtgagcggc gttatttatg gcaacaatgc ccaggcattg gaaaatgtac cgttatggca
     4801 aatcatcctt tttgcgcctt tgctgctgat ttccacgtgg gcgcctattt ttgtggtgat
     4861 atccggaatg gcgcaggcct atgtgttaag cggactgatt ctttccaaaa aagccgcaaa
     4921 tgccctctcc ctcaggaact tttttggcgg tgtcctcgtc accagcctgt ttttatacct
     4981 gctatcgctg gtaaatatgg cgtttttcca tcatccgatg gactatgcag gtgaattccg
     5041 ctataccttt ttgacgggag cattgcatgc cggacattgg gttccctttg acatgcgtct
     5101 gcttttttac aacgatgcgc tttccatgat ttcgattaac ggactactgg tcacctgtat
     5161 cctgtatttc ctatggaaaa tcaactggat tcctcgaaca agaaaagcct ctctgctgtt
     5221 actgggtgtt gccgcttctt ttttcgctct ttcaccgctg ctgcaccaga aattggatcc
     5281 gatttttctg acagccgttg aaaatggtca tgcaggaaca gcgtttttcc tgcgattctt
     5341 cgtgggcata ggattttcca cattccctta tgcggcatac ggtctgacgg gtgccatttt
     5401 aggtctgttt cttgcaaaac gcaaggaaga aatatggttg cgtcgtgacg gataccgtgc
     5461 tgctacggcg atggtcctct cttccgtcgt actcatgggc atcaccggat ttgctcccaa
     5521 agatgtcatt catcacccgt acccctttaa aatgcagctt ctggtactgg gcaccatcct
     5581 gctgacttgc atctggctta tacttaagtt ggaatacttc actgaagaac agcgtgccta
     5641 cagagctaaa aaaacggtgt ggctccgccg ttttggcttg cttgccctca ccattttctg
     5701 ttttgaaagc gttctctcca tgctgttcag caaggcgtac tgctttctat ggggcttcga
     5761 tgaagccttt cccaagacag ccccgcacat tttgtttttt ctcgctttga atgtgctgtt
     5821 ctggacattc gttctgaaat tctgggaaaa aatcaatttc aaatacagta ttgaatggtg
     5881 gatttcctgt ctggccggaa ttgtacgtgg ccgcccttcg gcaagattac aggcagatgc
     5941 tgttctgtac aagccctgcc ttctcccgga acctgtcgcc tcgccatgtc ctgcaggtct
     6001 ggaaaaccga taagccgctc tgctgccacg ctctgtatcg ttcctcagct gcgcctaaac
     6061 gccggcgcac tgactcccca gttcctgatc atgtagtccc gtgcttcacc gcatgcatag
     6121 attgagccgg taattaaaag cggtttttcg ggggaagctt ctctttttcc cgcctccagc
     6181 gcctcggcaa gagaaggtac aattatacat tgagcggaat cggcaaattg acggagcgct
     6241 gttaaaggaa gacttctttc accggtgtaa caggttaaaa taaggggccg agcaattcgc
     6301 ttcagcaagg caagcatcgt cgccgcgtct ttatcggatg acacagcaaa gactgcggta
     6361 cagttgtcta cggcttccac caacgcggcg caacctgccg gattatgagc aacatccatg
     6421 agtacaggtg gtgaatccag gagcctttcc agccgacccg gccacaccgc tttttccagt
     6481 cccgaaataa tagcgctgtc cgtcagccgt ggaaaatctg attgaagctg taaagccacg
     6541 gtaagtgcaa tggcagcatt ttcgatctga tgcattccgg caagccctag ctgcacattt
     6601 tcaaatactt tgccgttccc ccggtacgtt aaaacaggcg ttaaaggaga gcctcctttt
     6661 tccgcttcat attcgatccc ccatcgaaaa agcggcacag cgcgggcagc ggcctcctgt
     6721 tcaattaccc gctgtgcttc cggatcaatt ctgcccagca cagccgggat attctttttt
     6781 aaaatacctg ccttttcaaa agcgatctct gccagagtgc tgccaagata acgtgtatga
     6841 tccaaagcga tattggtaat ggcacaaatg cggggctgca ccacattggt tgagtcaaag
     6901 cgaccgccca tgcccacttc taataatgcc acatcgactt ttttcttttg gaaacagtaa
     6961 aaagccatgg cggtattcgc ttcgaaataa gtggggacgc aaagagcgcg cgcagacatg
     7021 agctggcacc agtgcgctga ctcttccagt gtggcattac agacaggcac tgcatcaatg
     7081 agaaaacgct cagtcacgtc aagcaggtgc gggcttgaaa aacgtcctgt cctgtatccg
     7141 gctgcaagaa gcatggcgtc cagaaatgca agaacgcttc cttttccatt ggtgccgcct
     7201 acatgtacta ccgggcaact gtattgcggc tctccccctt ctcttaggat ggcttcaatg
     7261 ttggaaagtc cgagtttaat cccatgcagt attaaactat ccaagtactc acgtgcagcc
     7321 agcgcatccg aaggctctgc gacaagcccg gtacccccat cggaaaggtc attctgcata
     7381 aaaatcaggc gctttcttca atggtttcag ctccagacag actgacatta tcggggtaat
     7441 acaaaatacg gtggcagtgc tggcaggtgt gcaccttttc gctgcccata atttcattga
     7501 caatttgcgg acgaatggtc ataaaacatc cgccacagga ttctgcattc aaaggcacca
     7561 cagcggggac acgtactttt cgaatccgct catacaaact gagcagttgg ggctccactt
     7621 cctctttgag ctcgggacgt ttcgcctgca gtgctttgcg gtgctgtaca agttcggcaa
     7681 gatcagcgtc tatcgctgcg cattcggcat ccagtttgct gatctcccct tgcacccgtg
     7741 ttttatcttc ttctaatttt gccagagctt cctcatgggc atccatgagt acaaggactc
     7801 tttcttcctt attgctgatc tgctttttct gcatttctat ttcatgaagc accgctttat
     7861 actcttcatt tcttttgata tccggaagct gaccttcata cttttttatt ttttcctgca
     7921 gctgttcaat cccggcttcg cactcacgct gctccgcctg aatcttccgg caatgttctt
     7981 cgctgttttt tatttcttca gcaagccttt ccttctgaat gaaaaattta tctttctgcc
     8041 gtggcagttc tttttctttt tggatacagt ccgcgatatt cgtatccagc ctttgaagtt
     8101 tcagtaagtt gatcaagatc agacactcct tgccgactct gttggaacaa aaaaaccgca
     8161 accctgaaag agttgcggct gatcgaagac gcttggggca tcccatttaa agaacggggt
     8221 tccgccaacg gaacataaag aggcgcgcag ccgccctgat tcagggccgc acggccgtat
     8281 tggcagattt atgggcatgg acgcagtatt cattcttttt ctgaattctg gtaaaacgct
     8341 tttcgctgcg aatggaattt cttcaactgt aactgcatat caacatacca tgcagtaaaa
     8401 cctgatgacg taaaagtata gcagaaaaaa gagaacaaaa aaaaatatgt gatccaacat
     8461 tgcagggcgg attcaaggct gaaatgcaat tcttgaatcc gccttacgac acaaacatcg
     8521 gataatcttg tttaactacc ctgaagacag gcataatgac cggcataagt atcttttttc
     8581 tgttctcaat aaggagagaa aacatggtgc ccttacttac ccggcgcgac tttttgaaaa
     8641 tcagtgctgc agggatcgca gccgccgcgt tgcctgtgcc ctctgttctg ggcgagcccc
     8701 ggaaaaccgg ttcctttacc tgggcaggca tcaatgactt gcatataaaa aaccgggact
     8761 gccttcctta tatttcacgt gtagtagcag cacttaacga taatccggct gttagtggcg
     8821 ttgttgtttt gggcgatctg gcaacaatgg cgcagccgga agaactgcgc cttgcgaaag
     8881 aagccttaaa acctctgaac aagccctgct atattcttcc gggaaatcac gatatcggcc
     8941 cggaaaccga aggcgctctg aaacgtttta atgatattat cggtccggag cactggcttc
     9001 ttgagtggga acattggaca tttcttggat ttaattcctg tgtcggcgct aagagcgacg
     9061 ttattcttcc ggaatccgaa ataaactggc tgcgggacca gctgatttcc atcgattctg
     9121 aacggccgct ggcgcttttt tgccatcatc ccctgaatcc tcattcaaaa gcttatcgca
     9181 tcattaacgg tgatgaaata ctggatctgt tttcgcatca cgccttacgg ctggtttctt
     9241 ccggacactg gcatgggaac caggaggaat ctaccgcgca gcacttgttt actacgactg
     9301 cctgctgctc ctctacgaga gacaactttg acaaaactgc agcgaaggga taccgcttat
     9361 ttcactttgg agaagaccgt gcagacaccg agttcgtcga agtccggtaa cccctgccct
     9421 ccaagagtgt tcagcctttt cttatcgcct cgttagggta ttgataagtc cgttttttaa
     9481 aaaaactgac ggtccttttt ctcctttccg cttcccgggc aatttgtatt tgaggtccgt
     9541 tttgtagtaa aattctatta tattactcag gatttaatga gtcttttcgg aggcgtaatg
     9601 aaacactcct tcgaacgatt cgtgttcacg tgcgggaggg aaggttctgc tttgtttatt
     9661 tgttttttta tgaacctgct tgacgatcag atgactttcc atgactattt tcctgaactt
     9721 tcaaagaaaa ctttttgcag gcattctgct tcttaccgtg cctgctgcgc ttcttttctg
     9781 gcagctgtcc catcctttta ctgaggcata cctcattggc cttgcagcag cactcatcag
     9841 cctgttgctg aaattcaggc ttgcccacat gacgctgaag cgattaacct acaccttgcc
     9901 ctggcatagc ctttgttcca tgtctctgta tatgatggct ttatacggcg gctacaaaat
     9961 tcatgcgaaa catttacttg gacttgcagg ggtctctatt gcgctcgggc tggtgctgct
    10021 tgtccttttt tttctggggc ttacagaatt ggatttactg ggcaccggac accacagttc
    10081 ggtttttcgt accggtcatc ggaaggatga gaaataatgg aagatctggg acaccgtctg
    10141 gtctggcatt atataccctt cacgaatctg gagatccctt tcggcggagt taatattctt
    10201 actttcgcca ataccgcttt tgtctttgtt gtggcactcg gtcttctgtg gctcggagca
    10261 agaaagcggg aaaccattcc cgggcgcgga cagatgcttt ttgaatttct gattcagact
    10321 ttccgggata tgctggaacc gtcggttaag caagaacatc ttcgcattgc ccgtcgcagc
    10381 cttccggtca tcatgtctct tttttatttt attctggttt ccaatgcaat cgtgctgctt
    10441 cctattccgc atgtggaaga gcctacagga gaccttaact gcacttttgc tctggcagtt
    10501 gtggcagtaa cctatgcgat tttatcaggc atccgtcaat ccggcctcaa gggtactttc
    10561 ggggaattct gcggtccttt ctggcatcag gaaaatcaga gtggctgggg tttttacgcg
    10621 gggaaattat ccgccctgtt ttttttcccc cttcgtattc ttgaggaaat cagccggatg
    10681 atttcgcttt cctgccgact gttcggaaac atttttggcg ttgccatcgt cattgcagtg
    10741 gtatcatcca ttacttacta cgttatggtt ccccttgcgc tgtatggcgt tcttgttgtt
    10801 tttgaagcgg gcctacaggc atttgtattt gccacattgt cggtcaccta tttatcatca
    10861 gcagtaaatc ctcactaact aaaggagaag ttctaaaaat gaatggaagt atttttgtta
    10921 cattcatgca atccgtcgct gctcaggctg aagcagctca ggcagcgcag ggcgtttcag
    10981 gcatggatct cagagttatt tttgcctgta tcagtgctgc actggtaatg agtcttgccg
    11041 ctcttgcaac cggtttaagc gaaggtcttg ctacgggaaa agcagtggaa agcatcgggc
    11101 gcaatcctga atcggcgggc ggggtaaccc gtgccttagt catcggcctc gccatcacag
    11161 aagctgttgc catttatgca ctgctgatcg ctgccatgat cctttttatg gtcattgact
    11221 gatccgttat ttctgtctgc acgcatgtta acgcttaatt tcacaagtgt tgttgcgctg
    11281 gctgtttttc ttattttttt aagaattgcc agtcgtattt tctggcgtcc catgattcgt
    11341 catatggtgc gccgggagcg gctttttgcc gttcaggaag agagcatcca gcagcagcta
    11401 tctgatgcca aagcactgca ggaacgcata cagcagcgat tggatcagaa aaaaaagatt
    11461 ttgagccatg acgtggatct gaaacttcag caggcccatt ccagtcggcg gttaatgctc
    11521 gcatcagaaa agaataaaac cgagcagcat ctggtacagc tgcatgatca gatgcaggag
    11581 tacctggaaa cagaacggcg ccgttttgcg gaagccctcc cgcgccttat agaggagatg
    11641 gatctccgct ttcaacaagg ggggccgcat ttatgaaaaa ccaacgtatt ttcttcgctt
    11701 ttttctgcat cgcctatgcg cttttgtctg caacagggat attcctcttc ggccgccccg
    11761 gctattccaa agaatatctg gcaaacaatc atgaggatca caagcgttat ctggctatca
    11821 gcaaaaaccc tctttatcag aaatattgtg agcgcccgct actgaacccg ctggatcagc
    11881 atctccagaa agaagcggat tttgccgcag cctacactgc ccgcccggca ttcagagctg
    11941 aacgtatgcg catgtttttg tatgcgatat ggtttaaagt gctgaatgcc ctgtttctct
    12001 ttatcctgtt tgttcgtttc gggctgccga tagcccgcac tttccttgac tcacacattc
    12061 atcaaataca gacaaaaaaa gatactttgg aagacgaact tgccagagca tcttcccagg
    12121 ctgctgaaag ccgggaggct ttcagtcatc tgcccaatca ggaagctgca cttgagcagt
    12181 cttttgatga tctctataag aaaaaactgg cagatattga aaaacaaagt cagcacgcac
    12241 ttgaacaact ggcaatcgat actgaaaaac gtattgcggc tgaagagcag gctgcagctg
    12301 ctgcagtacg ccgtgaactg gtagataacg cactgcatga actggagagg aaataccgga
    12361 aagaaccttc tcaagagcat ttaattaaaa gtgtggaaca attctgtcag tatatggaaa
    12421 tcatttcatg agaccggagc aaattgccgt aaaatacacc cttgccttgc gggcgaccct
    12481 tcatactgaa gaggatcttc atcgcacggt cgccacttac cggcaattta tgacaatctg
    12541 cggaaaagag cccgatctgc tccgcacttt aagcaattcg attgtcccgc tggatcacag
    12601 aaaacagctg cttgaaacag cgctaagcat atgtaatccc ccgccctcat tgacagcact
    12661 ggcattttta ctgctggaga gaaaccgttt ttctctttta cccgtcatcg cccggcaatt
    12721 tgaacagcag atggatcagt ggctcaatcg tgtggaagta gaggttatta cagccatccc
    12781 gctttcggct gagctgtttc aaaaagtgca tcacaccatg gaactgttta cgaaaaagac
    12841 tgtgcgtctg gttcagagca ttgaccctga tattatcggc ggacttatca taaaaatgga
    12901 agggctggcg atggatttca gttacagggc ccgactggaa aaactgagag aaaaacttct
    12961 ttcagaggga agaaaaattc atggcgctta atccacgaac agacgaaatc gttgaaatca
    13021 tccgacgcca gatcagtgat tttatctggt ctgctgagac ggctgaagtt ggcacggtca
    13081 ttcagtccgg agacggtatt gcccggattt tcggtttgga tgatgtggca gccggagaaa
    13141 tgcttgcatt cccgcatggc gtcaaaggga tggcctataa tctggagtcc gaccatgtcg
    13201 gcgccatcct tttcggtgac tatcacaaag tgcgggaagg cgacactgtc aaaagaactg
    13261 agaatatcac ctccgttccg gtgggtgaaa gcctgatcgg acgagtcgta aatgcccttg
    13321 gtgagccggt tgacgggagc ggccccctca ctaacacaac cgatctgccc gtggaacggc
    13381 tggcgcccgg cgtaatcgac cggcagcctg tgaacaaacc gctgcaaaca ggtataaaag
    13441 ccattgattc gatgacacct ataggaaaag gccagcgtga attgataatt ggagaccgtc
    13501 agacgggtaa aaccgccatc gcactggaca ccatgatcaa tcagcgcgga aaagacgtta
    13561 tctgcattta tgttgccatc gggcaaaagc agtctaccgt ggcacggctt atgcaggaac
    13621 tcgccgatca tggtgcaatg aaacactcca tcattgtaac cgcttccgca tcggaacctg
    13681 cgcccatgct gtatcttgct ccttacacgg gctgcactat aggtgaatat ttccgggata
    13741 gcggtagaga tgcactggtt atttacgacg atctgtccaa gcatgccgtt gcgtaccgtc
    13801 agctttccct gctgctgcgt cgccctcccg gccgcgaagc atacccggga gacgtttttt
    13861 atcttcacag ccgtttgctg gagcgtgcgg cggctctgag tgaggaaaaa ggcgggggca
    13921 ccctttccgc cctgcccatt atagagactc aggcgggcga cgtttccgct tatgttccga
    13981 cgaacgtcat ttccattacg gacggacaga tttatcttga atcctccctt ttccacgcag
    14041 ggatccggcc cgccattaac ccgggcatca gtgtcagccg tgtaggcgga gcggcgcagg
    14101 cggatgccat gaaaaaggtt tccggtcctc ttcgtttaaa tcttgcccaa ttccgcgaac
    14161 tgcaggcttt cgcgcagttc ggcagtgaat tggataagac cgcacaggca cagctggagc
    14221 gcggtgaacg gctggtcgaa atcttaaaac aggatcagag caaaccgctc tccgtttctg
    14281 ctcagattct gatcatccgc tgcgcatcca tcggtctttt ggacgacatc gctgttcggg
    14341 agcttgaccg gtttgaggaa gaactatttg cattctttaa cctgaatcat gaagcgctgc
    14401 tggaagagct cgcctcaacc cgacattttg acgaagctct taaacagaaa ctggatgaaa
    14461 gcctgaagca ctttaaagaa cagttcttct gcggagaatc ccggacagat ggctaatctt
    14521 caggaaatcc ggcgacgcat aggcagcgta aaaaacacgc agaaaattac ccgtgccatg
    14581 aaacttgtgg cggcgtccaa agtgcgccat gcccaaaaag cactggctac ctcacgcact
    14641 tatgccgctc atgtgtactc gttagcagaa gagatgcttt ttgaatcgca ggattatgcg
    14701 catcctcttt tggaagagcg cggaggaaaa agtgcctgcc ttcttgtagt cacctccgac
    14761 cgcgggctgt gcggcagttt taatcattct ttgtttcagg aggctgcgca gtggtggcaa
    14821 acccaccgcc gggacaaaga agtttttatc catgtcatcg gcaaaaaagg aatggacctt
    14881 tttcaacgca gtcgcattcc tgtacaggaa tacagtacag gcgtgctgga aggcaatccc
    14941 gttcacactg caatagacct ggtcaatagc tatatccgcg gattttcagc gcaccttttt
    15001 gatgaggtct actgtttata cagccgtttt gccactgtgg tcagtcagga aatctgtctg
    15061 gaaaagatgc ttccctgccg cttcgaaaaa acagacacgt cggcgttttc cgactggatt
    15121 atcgagccgg acaggagagc ccttttgtcc aggctgatca catccaacct cgttgcccag
    15181 caatacagga ttctccagga gtctctagca agtgaacaag gcgcccgaat gaccgctatg
    15241 gaatcggcaa ccaataacgc cggtgatgtg cttgcttcgt taacactcag ttacaaccgc
    15301 ctccgccagg atgccatcac ccgggaagta gttgaagtga tcagcggtgc atcgagccag
    15361 gagttttgag ggataaaatg aaaacaggaa aaatcaccca gatcatcgga cctgtaatcg
    15421 acgtccagtt tgaggcgggc aatttgccgc ctatcaacac ggcacttgag gtgagacctc
    15481 ccaaaagttc ggtggtcacg ctggaagttg cactgcatct gggagacaat gtggtgcgta
    15541 ccattgccat gcaaccgact gaaggtcttt tgcgtcggat ggaagtggtt aatcttgaac
    15601 agcccatcag cgttcctgtc gggcgcgaga cgctcggacg catcctcaat gtgatcggtg
    15661 cacctgtaga cggactcggg gcggtcaata caaaagaacg gtggcccatt caccgcagtg
    15721 caccggatct gaaagatatt aacatccgca ccgagatttt cgaaacaggc atcaaggtga
    15781 tcgatctgct tgctccctat ccccggggag gaaaaatagg cttattcggc ggtgcgggcg
    15841 taggcaaaac cgttctgatt atggaactga ttcataatgt agccatcaac catggcggtt
    15901 actccgtttt cgccggtgta ggcgaacgaa cccgcgaggg caacgacctc tggcgggaaa
    15961 tgaagcagtc cggcgttctg gaaaaggcgg cccttatttt cgggcaaatg acagaaccgc
    16021 ccggcgcacg ggcccgtgtt gctctgacgg ggcttacggt tgcggagtat tttcgtgacg
    16081 ttgcgggaca ggatgtgctt ctttttattg acaatatctt cggcttcacc caggcaggtg
    16141 ctgaagtatc gacgcttctc gggcgcatgc ccagtgctgt cggctaccag cctactcttt
    16201 ccaccgaaat gggtgaattg caggaaagga tcacttccac gcgaaaggga tctattactt
    16261 ctgtgcaggc cgtgtacgtt ccggcagatg acctgacgga cccggctccg gcaactacct
    16321 tttcacatct ggatgcaacc accgttcttt cccgcaaaat tgttgaatta ggcatttacc
    16381 ccgctgttga cccccttgat tcgaccagcc gtattctgga tccgcgctac gtcggtgaag
    16441 agcactaccg gattgcccgt gacgtgcaaa aagtactgca gcgctaccag gatcttcagg
    16501 acattattgc aattctcggc atggacgaac tctccgaaga tgacaaggca attgtcggac
    16561 gtgcccggcg cattcagcgt ttcctgtcgc aaccgaattt tgtggctgaa gaattcaccg
    16621 ggatcccagg aaagtctgtt ccgctggaag aaacactgca cagtttcaaa ctgattctgg
    16681 caggcgacta tgatcattac cctgaggcgg cctttctcta ttgcggcggc atagaggatg
    16741 tcgttgaaaa agcaaaaaca ctgggctcgc atgcatcatg attcaaaccg gctctgctaa
    16801 aagattactg caggtcgaaa tttgttcaat ggacaaggcg cctgtacgtc tggaagcgtc
    16861 cctggtcaaa ctagaaggcg gtctgggccc ctttaccgtt ttgccagggc atgccccgat
    16921 gcttgcctcc ctggacatcg gcgtgatgac attgcattct gccgacggtg agcgtttttt
    16981 ttacgctgtg aatggcggtg tggtaaaagt ctgtaatgat caggtactca tccttccgca
    17041 gagttttgaa gaaagcggcg aaatagatat ggagcgggcg caacaggcca aaacaaaagc
    17101 agaaacgatt ttgaaagagc gccattcttc cgagctggaa cggatggagg ttgtgttaaa
    17161 gcgcgccata gcgcgtattc gtgccagaag ctattctgaa gggaaagtgt gatttttctt
    17221 tcatcgctga tttcctgaag tcctcttttt tttctgctat actttacgga ggctttgtaa
    17281 aaacatcagg aacccagcgt acttgctctg ccaggcaaag gtcttcgtct gcccgctcaa
    17341 ctaaattatg gacttatttt catccacatc cccagctgtc aaagtcgcgc ttcctctgag
    17401 tctggataag gtgctgactt ggcttgtgcc gcctgaactg gtcgataaag ccaggcctgg
    17461 gatgcgggct cttgttccca tccagtccag aattgtttta ggaatcattg ttgaggtgct
    17521 cgcagaaagc gacgtgcaag aagccaggac gctgattgat attcctgatc atgaaccggt
    17581 tcttgataaa aacctgcttc ggctctgcaa atggatcgct gactactatt gctgctcctg
    17641 gggcgaagcg cttttttctg cgctcccttc ttcgctgaag gcggtgccca aaaaacagta
    17701 ttacctcaat gaaaacaaac tggattccgg cagattatca gaaatgcagc gcaaaattgt
    17761 cggcgctctt tatagacaga gtccgcagac cgtcgtacaa ctttcaaaat ctgtaggaac
    17821 aggcgctctg caaagcgccc tcagatccct gctttccaga aaaatcattt ttgaggacca
    17881 tctgtttcaa gaccgcgccc gctccattct cacagaaaca cgggtttccc ttgcagctga
    17941 tgctccgggt gatcctgaaa cactgcaggc gcttcagaaa aaagcaccaa agcaggcggc
    18001 actttatcta gaattgctgt atggggaaaa agaacagtcc gccgatctcc tctgccggaa
    18061 gcacaataca acgaaaagta cgtttaaaag tctggaaaaa agagggtggg tacacttgca
    18121 ggaaacagaa gtctaccgtc gcccttctct gcccgccggt tttcacgcta aagacaaatt
    18181 caaactcaat gaagagcagc tcaacgctta caatgccatg tgcacgcccc ttttaaagcg
    18241 ggagtatcat tgctttctgc tccatggcat tacgggatcc ggcaaaacag aggtttatct
    18301 gcaaattatt gaaaaagcgc tgtccgtcgg aagaacagcg ttgatgctgg ttcctgaaat
    18361 ctctctgacg ccgcagacgg tcggccgttt ttatgcacgt ttcaatcagg atatcgctgt
    18421 ttttcacagc ggtctcagtg agggtgaacg ctacgatgcc tggcgccgca cccgtgaagg
    18481 gaaagtgcgc attgttgtcg gagcaagatc tgccatcttt gctccgttgc agaatatcgc
    18541 tgtcattgtt gtcgacgaag aacacgatca ttcttataag cagggagaag caccccggta
    18601 tcacggcaga gacgttgcaa ttgttcgggc ccgaatggaa aatgctcttt gtgtcctcgg
    18661 atccgccacg ccgtcggttg agtcttttta taattccgag caaaagaaat cttcccggct
    18721 tgtactgctg aaccgcgcca ctaccagttc ccttcctgaa gttcgtatta ttgacatgcg
    18781 ccgggaagtg gcagaaactc cgggacaact gattctttcc accgaattgg agaaagctgt
    18841 tcagaaacgt gttgcggcga atgagcaggt cattcttctt ttaaaccggc gcggctttgc
    18901 acctgtggtt ctttgccctg tctgcggatg gataccggaa tgcgataact gtcaggtcag
    18961 cctcacctat catcgtgcag gcggaaatct gctctgccat tactgcaaca caagccgctc
    19021 caatccgctg gtctgcagcc aatgcggctt caatcccctt ctttatcttg gagcgggcac
    19081 ccaaaaggca gaggactatc ttgtccgctc cttttccgga gcccgtgtcg agcgcatgga
    19141 cggcgacaca acatccggca aaggtggtca tgctaacatc ctggagcgtt ttgcccgccg
    19201 ggaaattgac atactcatcg gcacccaaat gctcgccaaa ggacacgatt acccgggcgt
    19261 aactctcgta ggtgtaatca atgcagatgc cggattaacc attccggact ttcgtgccgc
    19321 tgaacagtct ttccagcttc ttacgcaggt ggcaggcagg gcgggccggg gcagcctggg
    19381 cggggaagta atcattcaga cccatcgccc gcagcactat gcggtgcaaa gtgcatcacg
    19441 ccatgattat gctgcttttt acgcccacga gatccaacag cgggaagcag cgggctaccc
    19501 tcccttcaga cgtctggtta attttctgat agaaggtgaa gaccctctgt atacggaaaa
    19561 agccagtctc ctgctgaggc ggctggcgtt ggcccaggca gatgaaatgg ggtttcacgg
    19621 gattgctttt ctcggacctg ctccggcatc ggtacggcgt gtcaaaaaaa tctatcgctg
    19681 gaatttcgcc ctcatgtcac gcagtgccgc cagagtgaac gctctttccc gagcgctccg
    19741 tgaagttttt gaaaataatg caccggaagg caacatccgc ctgaaaatag atctcgatcc
    19801 atacggcatg ttttaggtga aaatggctct gctctgcgtc tttcagcaca aaaaaaaggc
    19861 ttacacctat gtgtaagcct tgagcattat tggtagcggg ggcgggactc gaacccgcga
    19921 ccttcgggtt atgagcccga cgagctaccg gctgctccac cccgcgtcaa tttctaaagg
    19981 cattgtatca gaaaaaagga ggcgacatca agggaattgg ggcagaattt cagcaaaaaa
    20041 cggttttttt tgtaaaaatt gaagttttgt ggttcgaaac ctctttattt tagtttcttt
    20101 tacctctgca gcccaacccg cgatcgataa gtctaacccg ctcaacactt cggtccgtat
    20161 acagcgcact cgtcccgaga atctcatcgc cgagcataag ccgcacattc ccgcattcag
    20221 tcccttccag aacaggagct tccagggaga caggggctgt tatttccaaa aacaaacgat
    20281 cgctctcatc tttgttgata agagctgtta tgtccgaggc ggcgcgcagc cccacttctt
    20341 cttccatact tttttgcaca ggaattgctt tgccaatgga catgccggca aacaccggct
    20401 gcactcgtgt tattttggcg aaacattcgt taagaatatc acgcgtatga ttaaaacgac
    20461 cttcccgatc gcttcccata agcacagcga tcaggcggat gtcatttctc ttggcggtgg
    20521 ctgtcaaaca gaagcctgaa gcccgaatat agcctgtttt caggccgtca cagtccggca
    20581 tgcgatccat gagtttattc gtgctgggcc ggggcggcgt attggggcgc aaagcaaatt
    20641 cccgggtact ggatcgtaca agcgtctgag ggtatttaag agattccttt gccagaattg
    20701 ccatatcccg cgcgcttgta agatcgaagt tctcgcggtc tgcaggcggt aagccatgga
    20761 cgctggtaaa atgcgtgttt ttcatgccca gttcttcagc gcgccggttc atagcatcaa
    20821 ggcatgcagg gacactgccc cacagagact ctgcaacagc tacagaggca tcattggcgg
    20881 aaagcaccgc catcgccatc agcatgtctt ccagaggaaa aatatcaccg gcagcgagat
    20941 agatctgcgt gccgcccatg ctctgcgccg cttttgaagt ggtaattggt gtatcgtagc
    21001 tccacttacc tgcatgaacg ccttcatcag ccagaagcat gaccatcatt ttcagcatgc
    21061 ttgcaggcgg tcgctgcaag tcggcatttg attcggtaat aatcttgccc gtctccgcct
    21121 caatacaaat ataacttgga ggcagttgtt catatccttg ctgaacagtc tgcgatgcaa
    21181 tataaataga aacggctgca attaaactga aggacattct tgcaaaaact cctcattatg
    21241 acttatgaaa tgcttcgttt ttaagcctgc ttttttcctg ttgtatatca cgctgttttt
    21301 ttatgagcgc tgtcatgtcg ctaatgggga aaggtttatg cagcacccct gcaatcccat
    21361 cgagctttgc ggcagcatcc accgtattgc gcatacctga aatcagaaaa agcggcactc
    21421 ttctggtttc gctgctgatg aaatacgaat taagcagtgc ggcgagctgc cctgtcggga
    21481 tatccgcatc gagaaaaacg gcatccgttt ttttctgacc taccagttcc aaaagacgtt
    21541 caggagctgt ggcaacccgt gcttcaatat tttgaagatg aagcgccttg ttcatccatt
    21601 ccagaaactc cgcatcctga tcaacgacaa ccgcctgcag tttatccgga tctaccagtg
    21661 ctgagggatc ttccagctct tttttataga cttcatctcc agtcagaata taaaggatct
    21721 tatcagcaag tgcctgggcg gtacagggtt tataaaagaa aaagacctgc gaatctgcat
    21781 ccagatcagt agtaatcttc tcgacatctt cattggtgag tacaagcaca gggagctgtg
    21841 ccagggaaaa ctgctgcctc aatatccgac gtgatgtgga gccggcaaca tccgcctgcg
    21901 catcattgat aataatgatg tcaaagtcat cggggtcttc ccatcgtgta acttcgtagg
    21961 cattggaagt gcaggtcaga cgacagtgcc gcaaagggga cattgtttta aaagcggctt
    22021 gccgttccgg actttcatcc acaagaagaa tcctgagcgc acccagcaaa ggaatcattt
    22081 cttcttttac gccggacaag tacgtagaat tgggaaagtg gacagtaaat gttgttcctt
    22141 tcccctttgc gctctgcact gaaatagaac cgttgtgagc ctgcactata tttaaagcaa
    22201 tcgaaagccc gatacctgct ccttcatact ggcgcgtttt ggaactgtct acctgaaaaa
    22261 atttcgcaaa aatcttctcg tgatatttct ggtcaatgcc aataccggtg tccgcaacct
    22321 caaaggaaac agtcctgtgt gttttcattg ctgcccgaac tgtaactttt cctcctttat
    22381 cggtaaattt tagggcattg ttcagaagaa tgcccagtac ctgacttatt ttgtctctgt
    22441 cgccccaggt ataaatggca ttatcaggca cttccacagc cagcgaaacg cctttttctt
    22501 cagctgccgg gacaagcgaa gtcattgctt cccgggcaag agcgctggca tcataaagat
    22561 ttgacgcaat ctgaacaccc ctgatttgca tgcggctgaa ctcgatcagt tcattaatat
    22621 gattcagcag atgccgggca ttgcgatcca taatgacgag ggcacgacgc tgctcatcgc
    22681 tgacagttcc catatcgtct tctttaagca tctcaatata gccctgaata gtggtcagcg
    22741 gagtgcgcag ctcatgggtg acattggaca ggaaactgtc tttggcacgg tcatgaagcc
    22801 ggagtccttc gttcgcccgt tcgagatctc tggcacgggc aaccaattgg atctgcatgc
    22861 gtttcagatc atctataggc gtgagaacca gcgagaagcc attgtctttt tcttcttcag
    22921 acggcagata agcaacaccg accagaacag acgtctccgg tttgccacct ttaagaagtt
    22981 ctatttcctg ccggagtgca tgtccgggct tgtgttcaat cgattttgaa aaaagattca
    23041 tgagacggga ccgccccacg ctgtccacga attcaaagat ttcccggcta atcagagact
    23101 ccgtgctggc attcaataat ttttccgcct gctcattagc aaaacggatc ctgtattgca
    23161 tatcaacagt taaaaatccc tcgttcattg aaagaagcag ctgacggagc tgttcttcgg
    23221 aacggtgaag tttgcgtgtt tgctcatcca ccagctgttg cagtccgcgg gcatattgct
    23281 ccacccggct cgtaagctgg cggtgttcgg taacatcgcg gattgttgcc atggttaggc
    23341 tatgattccc ccgcttatca aatacaggtg cgccattgag gagcagaatc ctggcttcac
    23401 cgttaactgt ccagttaatc tcgtattccg atgctacacc cacagcacgg ttatccaact
    23461 ggcgtgcaat cgtctcgaag ccaaaggata atgccagttc gcgggcattg tgtcctatta
    23521 ccgattggcg ggattttcca aaaatatcaa gggccttctg gttcacgaga agcacggtgc
    23581 cgtccggcgt actgagaatg aagccatcag agatgtgttc agccagaaac cggaaacgct
    23641 cagccagttc ttcaattccc gcgcccttgc gcatgatact cataaacacg acaacaaatc
    23701 cgacagaaag gctgattata cctatatatc gggcgaggac attgatggaa tcggctacat
    23761 attcaacttc actgagccga tcaaatacgc ctgagaaaac cactgaggcg ccagtcacca
    23821 gaagcagaaa ccccagtgcc atgccgaagc gttccatggg gcttgtgtaa tagcggacga
    23881 gaaggctgct tcccgtatag agcggcacga gcacggagag gaaaagcaca aacgcccgca
    23941 gcgccatatt atccagttgc tgcgaaaatg cgaccccgcc aagcccaatc agcacaggaa
    24001 caatgtagta ggtcagtctg cgtttgaaaa cggttgtcag attcatagca cactcttttt
    24061 tacaaaacga aacagccttt tcatcggggt gcctcgtagt ttgctgaagc ccgctgctct
    24121 tcaatcgctt ttgctgccag ccggtcgcag gcctcattta attcattgcc cgcatgaccg
    24181 cgcacccaga cccattctat ctcatgagaa gcagatacag cgtccagctc ttcccacaga
    24241 tccctgttaa gaacaggttc atttttgcgc ttccagttat tgcgcttcca tccggcaagc
    24301 cattcctgca tgcctttcac cagatagttg gagtcggaat acagaacaac tttacaggga
    24361 cgcttaagcg atgacaatgc ggctatagcg gcggagagtt ccatgcggtt gttggttgaa
    24421 tgaggctggc cgccgtatat ttcttttcga cgttcgccgt acagcagtat ggcaccccag
    24481 ccacccacac cgggattcgg ttcacatccg ccgtctgtgt agatgatcac ttgtggcttc
    24541 gtcatcccaa aaactcactc ggtttcagaa aatattcttt ttccattctt tacttcatta
    24601 taccccttta tatggtgttt ataaaatctt taatcgctta tcaatacaac atatctgttt
    24661 tcagaccact caagccagtt gtaaaatcat aattttttaa aaaagcgcaa catgttgcac
    24721 tttttcctct tctatggtat tataaggtca atatgttgtg gttcaagtta tgaacataca
    24781 atatattgtg tatttatgat tccagatagc accttacgac aaagggtaga cggtatgcaa
    24841 ccaaagatgc aacgggagga aaagaaatga cacttgtccg acttgtttat ctgcctggtg
    24901 aaccggcaat caattcttta tgccccaagt cggcgcttct ggaaggtgta ggtcagcttt
    24961 gcgaggcagg ctttcaaacc gatcttcaac accttggaga agtgcgctcg catggggagg
    25021 cgttgatatc tgaaacgccc cgcgttgcat tgaccccagc cgaagcagtg agcggccttc
    25081 ccgccgccgc attaacggtg ttttatgcag aatcccgcgg ggactatgaa caagtccgca
    25141 aagcgagtct gctcctagcg aaatatcatc cggagacaac gcaattactt gccggtccct
    25201 ttgcggtaca ttttgcaacc tgtgctcttg ctgaccttcc ggttttagac ggtgcgattt
    25261 cagaccatct gtctgctacg cttccacagg ctgcgcttta tcttctggga ggacaaaagc
    25321 gcccggacag cggcgacctg tcttttcagg aaaatggcgg gaaagcggta tggaaacgcc
    25381 gggccgcctt tactactctt tctccttttg tatcaagaag acgctgtgag aaaaacaatc
    25441 tccttcaata ctacccttta tctttttcag aagggccatg cgcatttttt tatcaggaca
    25501 aaaaatgcag tcagcgcgga tccaaatcag ccgcgctgct ctttaatgaa attaaatggc
    25561 tccatgagag ccatgatgcc actgtttttc acattgatat tcaacatgtt gcatcggaag
    25621 aagtggaaga acttgcccag cgactattag accacaacct gatgattctc tacagtcttg
    25681 gaaacgccac acagtctttt tcccgcacta cggcaagact gctgcttgcg tccgggtgcc
    25741 ggagtatcgg tttcaggata ccgacgggca gtcagcgcct cctggaagat ttttacggta
    25801 tgcaggtaag tatcagcgcc atgcagtcag tgcttcgcct ttgccgtgat accggtctgc
    25861 agaccgtcct ccatctgacg ggaccctgtc cgatagatga tattcatagc acggcggaga
    25921 ctgtacgctt tcttgaagac gcacaccccc atagcgtaac cctctctccg cttgccgttg
    25981 taccacactc actttggttt tgtcagcccc gtcgctttgg tctttccttc agctataagc
    26041 actaccagaa acagatggat cctttccgcc ttcctctcac gcttgccggc aaatggaata
    26101 ggtatcgtct tgcttttgtt aacaaagtaa ttcaggatta cgctttcaaa gcggggcttc
    26161 tttttaacgc tgacgagcgt tcgatgctcc tgctgcgctt gcttggtatt gagggcgtga
    26221 gtgactcaag ccgctttctg cagcatcttt cgcggctgct ggagagtaac gacgctgccg
    26281 gtctgtctga tgtcatacag aagtttaacc accgtgctac agtagcagtt tccagggatg
    26341 cggcggcgat gccccatttc ggtcaggttg cgatttcccg ctgagcacgc ggggttatta
    26401 atttccggcg aacaggcgca ggttgctgcg cgttttccat gtgtccagtt ggtatttttg
    26461 ctgtcgatcc tgtataattc acggcagtca aaaagaaaca cagatccaga taaaaagggt
    26521 attgcgatga gtaacgttgc tgaacttaca gaagccactt ttaaagatgc agtgaatagc
    26581 ggtgtttcgc ttattgattt ttgggcagaa tggtgcggtc cctgccggat gatgggtcct
    26641 atcctggacg atgtcgcaaa ggcagtcggt gatacagcca atgtctataa agtgaacgtt
    26701 gacaatgctc agaaccttgc ggcgactttg aatatttcaa gcattcctgc gctgattatt
    26761 tttaaagacg gcgaagaagt caagcgcttt gtcggagtca cttccaaagg tgatcttatc
    26821 agtgcgctgg aagcggcggc agaatagttc ttttctgcac cgttaatcca aacattcaag
    26881 gcgctctgta acaacaatga ggtacagggt gccttttttt aaagtcctct gtcagacaat
    26941 tgacatactt ttcttcggca gaacttaata agacaaccaa aaaaggacac aatgatgagt
    27001 acaaaagtcg tcgccgttgt cggcgcaact ggtgttgtcg gtcgccaaat gctggaaact
    27061 ctggaagcgc gcaacttccc gctctcccgc cttgtccccc ttgcctccga gcgttcccgt
    27121 ggcaaaacag tttccttcaa aggggagaca attcctgtgg aagttttgtc cgaaaactct
    27181 tttgaaggga tcgacatagc gctgttcagt gcgggcggtg gcaccagtaa aaaatacgca
    27241 cctctggcgg ctgcctccgg tgcagttgtt attgacaact ccagtgcttt tagaatggac
    27301 ccggacgtgc ctctagttgt tcccgaggtc aatgcacatg ctttaaaaaa tcacaaaggc
    27361 attattgcaa atccgaactg ttcaacaaca cagatggttg tcgtactgaa gcctctgcat
    27421 gatgctgccc ggatccggcg cattgtcgtt tccacctatc aggcagtctc cggtgcggga
    27481 attgcagcca ttacggagct gcaggagcag tcccgtgccc ttctggcggg tgagccttat
    27541 gcacccaagg tttttccgca ccagatcgct ttcaactgcc ttccgcaaat tccgcaaagc
    27601 gatgcttttc ttgacaatgg atataccagt gaagaaatga agatggttca tgagaccaga
    27661 aagattctag gtgatgacag catagccgtc acagcgacaa ctgtccgcgt ccctgtcctg
    27721 aacgggcaca gtgaaagtgt caacgttcag accgagaaaa agctgacggc agcagaagcg
    27781 cgtgcgcttc tttccaaagc acccggagtg gtcgtacagg atgaccccca gcaccaaatt
    27841 tatcctcttg ccgtaaattc aaccggaaaa tttgacactt ttgtcgggcg catccgggac
    27901 gacatctctc acgaaagtgc tctggacatg tggattgtgt cggacaacct gctgaaaggt
    27961 gctgcgctca atacggtaca gattgctgaa gagcttcttt aagcccgcga tacacttacc
    28021 aaaacgcaaa cgcccgggtt cggtctcccg ggcgtttttt ttattcgctc cgtgatgccg
    28081 tataaagtgt gacgaccccg aaagtcatgg gtcgggattg cacgacttca aaccctgcat
    28141 tgtaaagatg atcacggatc actgagggac tgggaaaagc ctcaaccgaa cggttgagat
    28201 agcgatatgc atctttttgt cggctcaatt tccccgctac cggcgtcata agatagcgaa
    28261 aatagaagag gtatgcgctg cgaatcaaat agttttccgg taagctgaat tcaagaatga
    28321 cgacctttcc gcccggtcgc aatacccggt aaatttctct gaagctttcg gaaagcagag
    28381 caaagttacg aattccaaag gcaatggtta ttgtgtccac acttcggtcg gcaaaacaca
    28441 gcgccgccgc atccgactgc ataagcacgg cttgtgtccg tccgccgtgc tgaagtttgt
    28501 tttgggcagc ctgcagcata ttaaaagaaa tgtccgcacc gaaaaccgca gcctcccccg
    28561 cttccgccgc cagccctttc aagacatcac ctgttcccgt cgcgatatcc agaatcacat
    28621 ggttttcatc cttaaagacg gtacgcagca tcgctttacg ccaggcgaca tcccggcgaa
    28681 aagagaatat tctgttgatt acatcgtatc gccgggcaat cgcatcaaac ataaaacgtg
    28741 tttgattccg ctgtgcgtga atcatgtccg gcttaaccgg tggttcaggc ttcatccatg
    28801 cctttcttta cagagatttc gtgcggttcc agccgcttaa acatctccag tgcattttca
    28861 ccggttcgtc ggtaataacc gtttcgatag cggaccgggc gaaatccaag cgtcaggtaa
    28921 agaagaattg cccggtaatt ggtctcgcgc acctccagac gtattgcctc cagtcccgaa
    28981 gcggaaacct ctttaataag atttttcatc agcggtcttg caaatccctg tcttctgtaa
    29041 ggcggcagca cagtcacccg ggtcacatgt gcctcatcca caagatccca gaatccggca
    29101 tacccgatca gttctccgta acggagcata acaaagaaat gagaccggat attatagatt
    29161 tcctgcagca gttcggaagt attccaagct tcagggtacg cctctttctc cgcttcttca
    29221 acaaaaggca ggaaataaaa atccaaggga tgaaatgtca aggcaatgcc gggaaattca
    29281 ttcatggccg gtgtttttct ggggtcgctg cttttgtacc cgcagcctct tttgcctgtt
    29341 cggcctggga caggcgatga taaagcggta cggcgtaagc gggattgtgt tccgtctgtg
    29401 tgagccacaa atcaagagct tccgctgcga ctgcatcggc acggggaagt ccgcaatgta
    29461 cttcggcaat catcgcttca ggtgcccggc tgcgtatttc actcgcataa cgctgtgccc
    29521 catcaccaag aaacacaaga ggcgctgttg cgctcagagc agcttcaata aaaaaatcga
    29581 cagacgctgc ctgatcgggc actatttttt cgcggcatcc tttttgaaag cggtaaacag
    29641 caccaaaaac ttctttcatc cgggcatcca gcaaaggcac taatgttcct tggggaaacg
    29701 cattcattcg cgcgagcgca tccagtgtcg gcaccgacac cagaggcagt tgaagagcca
    29761 aagcaagtcc tttccaaaaa gacagcccta cgcgcagtcn nnnnnnnnna atgagcctgg
    29821 tccggtagtc accgccagac aggagagact ttgcaactct gtcccggcct tttcaagaac
    29881 cccttccaca gcttcaagca gacgctcgga atgcagatgc cgactatcca gccccatagt
    29941 gctgatggga atgcatgagg gtttgttttt atcacattgg cataacgcca ccgtgcaata
    30001 gcccgtgctt gtatctccgg caagtatcaa aggaaaggtc tcagtatttg agcaggtatg
    30061 cgccatgact ttatggtttt cttgtcttag gtcgggtaaa ttccgccggg gaggcggcac
    30121 gctgcagcat ttccctgccc ggtttaaaaa caacatcgcg gtaggaaggc acagcgactt
    30181 tttcgcctgt atgaatgttt cttcccaggc gcggagcacg ctgtttggtt ctgaaaacgc
    30241 caaagttgcg tatttcccag tactcacctt ccagcagggc attcgcaaga atagaaagaa
    30301 tcgtctccgt tgatttgcgg gcagcctccg gctgcagctc aagacgctca gccatctgtt
    30361 gagcaatgtc tttttttgtt gtagtcatat agaagaagtc cttaccttag atgcctggcg
    30421 ctgtttcctg tacgggcatc ttcctttatc ttatatggct tcaaaatatt tttcgtaggc
    30481 atgtattgga agaatgtgca tgcggcacag caatttaaaa ggccacttaa acaacttcac
    30541 ccatgaatag tagttgggat aatccagaaa gtatttattg ggggcggcga tgatggcttc
    30601 aaattcttct ctggacatct gctgtttttc caggctctga cttacaaact cttcattatc
    30661 gaaatacggc ggggatgcca taatagccag cccctcttct cttgtcaata tgcctgttcg
    30721 cacctgggca gccacatcga ccactctcca gtcaatgccg aatttccgcc aggcatagtt
    30781 ataaatgaga ataaaaagct cgttatccat atggtgcccg tcaggctcct gccatccata
    30841 acgctcaatc aacgtctttt ctatctgacg tcccatgtca ttgtaatagt tcgtcacatt
    30901 aacaactttt attctcttta taaaaagcca ataaagaaaa tgatgaatat cggcattttt
    30961 aaaatgattt atttttgttt tgacaaaccg cttgatgatt gcacgggtat agcgggcgtc
    31021 catgtaattc cacaacagcg gtgccacacc ttcttctctg aaagagtgac ttaataaaat
    31081 gtacttaatg tcgttttttg ctgccgtgct atacaaggta taagcaatgc ctatgtcgtt
    31141 ggtcaaatca acataaggaa cacaggcacg aatattggca ttcgtcaact ctctggaatc
    31201 atcccagtcc atcgtgacaa tatgcaagtc aactccaagt tttgcgcata tgttttccat
    31261 gttggaccgc gctttttctg aagtccagcc attgtcaaag tgaaccgcaa gcggtttcag
    31321 acccagcact tctttggcat aatggaggca ataagaggta tctc
//
LOCUS       DAOPWB010000015        31024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1393_length_31024_cov_5.224967, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000015 DAOPWB010000000
VERSION     DAOPWB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 31024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31024
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1393_length_31024_cov_5.224967"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKN92_02765"
     CDS             <1..296
                     /locus_tag="PKN92_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47745.1"
                     /translation="YSTRYPELPAILAENPKAPCGNLIARNICLGGTWDEVEDKARDL
                     LRFEDNLLNDPSCFADAKAGDYSLPPDSPARAKGIREIPVNEMGTYSHPDCAF"
     gene            complement(304..1176)
                     /locus_tag="PKN92_02770"
     CDS             complement(304..1176)
                     /locus_tag="PKN92_02770"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNZ47746.1"
                     /translation="MGRFSDWIRLLRVYQWTKNALVLMPLVFAQQLLNPEAAVRSLLA
                     TVAFCLASSATYIVNDLRDRESDRNHPTKRMRPLASGAVAPVPASLFSAALLVLSLAL
                     AFHLNWAFLLSLLTYLALTASYSFFLKDLFIVDVIAVALGFVVRAIAGAVVIDVVFSN
                     WLIVCTLFLALFLGLGKRRGEIALLEEDAADHREVLHHYSIDFIDKMLIVVAGGAMIT
                     FTIYTCSPEVVSRIGTDKLYMTLPFVIYGLARYLWLVEQNETGDPSQVLLKDLPTCVA
                     VLLWAITCALIIYS"
     gene            complement(1190..1948)
                     /locus_tag="PKN92_02775"
     CDS             complement(1190..1948)
                     /locus_tag="PKN92_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ47747.1"
                     /translation="MTAISRKTVVIVGALSSVARAVADQFARQGFSILLAGRDVEELR
                     RIAKDLTIRHEVPCHVRFFDALDNAGHSSFLDQCSESFGNLPGGIVYCAGYMADQQLA
                     QMDTAAMRRTMEVNLLGAISILEKFAAAFEKHGSGFIAAISSVAGERGRKSNYIYGAA
                     KAGLTAYLSGLRNRLYTANVQVTTILPGFIDTAMTFGLPLPGPLVASPEGAGKVIVKA
                     ILRGANTIYVPFFWRWIMLIIRNIPEWQFKKMSI"
     gene            complement(1963..3336)
                     /locus_tag="PKN92_02780"
     CDS             complement(1963..3336)
                     /locus_tag="PKN92_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNZ47748.1"
                     /translation="MEGRSVLLNTASINLAGWGRYHPKKCSVFRPERTSELKNFLEES
                     GDMSFIARGLGRSYGDTAVNSAGLALDMSRMNRMLYFDEEHGILECEGGVTLAEILDA
                     FVSRGWFLPVTPGTKFVTVGGAIANDVHGKNHHVDGTFCQFVDSFDLWVPSKGVLTCS
                     ASENSDLFWATAGGVGLTGVILKVRLQLRAVETAYVKVDYRRCAHLDDVLEKMAATDQ
                     DYLYSVAWIDCLARRQQLGRSVLMQGAHARLRDLPVRLERNPFRLPKKAPLAVPVDFP
                     GFVLNNWSIKAFNTLFYLLNRDKEQAIINYDKYFYPLDGIHDWNRMYGRRGFIQYQAA
                     FPYEGRTGLVKMLELLSEKKRASFLAVLKCFGASNPGLLSYPMPGYTLALDIPCKKGI
                     EDFTKELDRILLDHGGRLYLAKDAVTDAATFASMYPALERFRSLRRELDPQQLLSSDQ
                     AKRLHLV"
     gene            3546..4271
                     /locus_tag="PKN92_02785"
     CDS             3546..4271
                     /locus_tag="PKN92_02785"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNZ47749.1"
                     /translation="MDFIEQEDSRHYSYGIGHMQQFSFQQQGAFFHTPRITWAVQMLI
                     LVNAACFALQLIAYPFETGLASSWLRLDMWFGFQPAAFLHGFLWMPFTYQFLHGGLLH
                     LFMNMLWLFVFGPDVERLLGSRQFLFFYIACGALGVLASLVPYLATGHGALIIGASGS
                     TMGVLVAFAMIDPQRQFLLFPLPVPVTAVWLVILVVIFNILMARSGNSGISVATHFGG
                     MLAGGALMKGIPRYHSWRRSRRR"
     gene            4323..4643
                     /locus_tag="PKN92_02790"
     CDS             4323..4643
                     /locus_tag="PKN92_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNZ47750.1"
                     /translation="MPGLPKIIFFAIVFYILHATGLLGFFRRLFDEVKKSMAQADAPG
                     KSAGDAEVCYRLLGVTPSAKWEEIEKAYRSKAKVHHPDLGGDEDAMRALNEAYNYIKK
                     LRKG"
     gene            4648..8985
                     /locus_tag="PKN92_02795"
     CDS             4648..8985
                     /locus_tag="PKN92_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47751.1"
                     /translation="MAPPVHPIDAPGGHESSSEGQSADTCDRTGTMNPRLRRILLVLC
                     LLIAAGIRCSLFLESRSGPDYAHPLHDAEFRDYWARAIVTGDWTPPPGREQPFADWLP
                     YPHAPGYSWLLSGIYRIFGLGYDAPRLIQMFLGLISILLLYDIARRLYNDKAGIAAAA
                     LMSLYFVFPFYEMDLGQTSLIVFLLLLLTLFGSKAVKKQHLLFLTINALLFGILLLVR
                     NEFLPFGIIFLGVHLFSYLKSKNWKKILAGTTLWCLFSIIPVTPVSVYNYQHAQVLPI
                     VGGEGTYVFYCSNNQWATGTNPDSPDLRALEKNDEWTFFSFPAIQEACARAQNITPLS
                     YRELQKHFKQLNRKYIATEKKRLFWLMLKKAALFWGPVEVDENKVVACEKDYFAVLRY
                     LPGFPPAMALFLLGAVLLFIRAIRKEKNCTKENLAAWTLLLLAMAGYSGIFVLFYVTA
                     RYRVPVIPFMLLFGAVAVSDWFEWLRRKAFKKAAVGLTAFLLLTALLSRPLVPFTPDT
                     ERWLDERRRLWVKTDRIDEGIADQEAWNIRHPQSPGGQYHLALLLIEKGDYEKALNCL
                     QRTIALDSGYDAAYYNQGIVLLKLNRPEEALESFRIRLDRVPNDEKAWFGLAQAQQML
                     GDTGLQREALDHALSLKPGYPEALVDRSTLFLQEGSRDRAQALLEKALEAAPGYAMAE
                     FNLGLLLAGEGSYSKALSFFESALTHAPDRADFHYNTGLALIQLKQYEEAVRHFHEAL
                     RLRPDYPEVYSNLGLSEARRHHEEEAKHAFDRAFELLPEDSDTLYNYGCSLNSLGDTA
                     GARRYFEAVLEQAPDHGKALFELALLDIKEKALDNALIRLNRARTILSGDPEVCYNLG
                     LIRQQQYASDEAQRLFLETLQLDPGHFGANRSLGTLLRSEGRSADALPYLKAALEASE
                     AADDSALHYEYGVALYDLRHFDEAGQALNEAAQLAPGSAAIRTHQALVSIARHNFGDA
                     VARFREAVALDPDNLLLRYRLGLAELEEGDAASAADTLAPLLKQLPHRKDLFCDYGRA
                     LAALSKHDEALQAYRVAWLLDPAYLIAINALAFSLSMSGRPAEALEWLDLALAQDPEN
                     QDALFNTGHALHLLGKLEAADRLFLALLRLNPHHSTALLNRGLILEMQQRPEEALAQF
                     EAALKENNGLALAHHHLAGLLRRKGDREQALRHYRQARELDPGNQSTAVNLGFTLVEL
                     GRLAEAKELFLQAQEQAPDDAEAYIGQGDVLLAENNLNEAEAAYRHALRLSPDNAVAL
                     KQYGFLLVRQGKSAEALEKFEAARAALPYDAGLYCGLGDVSFARGELERAESYYQKAV
                     ELYPDFALAWNNYGDLLARKGQTQEAISAYLRARALEPENTSVLLNLGRLYAREKEAA
                     PAVEVLETALRLEPDNLRVRALLGDMRMLTGDAPSAIREYQRILLERPDWQEIRDKLD
                     TARAQAAEN"
     gene            9198..10046
                     /locus_tag="PKN92_02800"
     CDS             9198..10046
                     /locus_tag="PKN92_02800"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ47752.1"
                     /translation="MNFSIADAEVFVPDQATMPEALSRVTHLAVGAHQDDLEIMAADG
                     ILKCFQQSDKWFAGVVVTNGGGSPRTGLYAHLNDDEMMAVRRIEQKKAAIIGDYGAQF
                     LLNHPSSAVKDGANQAVVDDLVAVLKATRPEVIYTHNPADKHDTHVGVLLKLIAALRR
                     LPKEERPKHLYGCEVWRDLDWLCDEDKVAFDVSAHENLQAALVGVFDSQVAGGKRYDL
                     ATLGRRRAHATYSASHAVDTAVAIIYAIDLSPLLTDDSLTLEALVEAHMKRFNEDVTG
                     RIRKMS"
     gene            10100..11773
                     /locus_tag="PKN92_02805"
     CDS             10100..11773
                     /locus_tag="PKN92_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HNZ47753.1"
                     /translation="MKQYIFVCLLFVALLFAGCGDTGRSTSGPGDILTEYMPDAMPEQ
                     GDVLRLLLPAEMPHLNPLTSTDYYASTVLEWIFDTLLDRDPATLESRPAIAARWEVSE
                     DHLAYTFYLRDDIVFSDGTPLTAQDVKFSFDTVMDPAVDAPNLRNYFIDIKECIAVDA
                     YTVRFTCDKPYYQHLIMLGGLSIMPKHIYGEGDFNRHPNNRKPVGSGMYTLEAWDTGQ
                     RLLLQRNPRYRGALEAGRPWFDRIEYAIIPDDNTAFQVLAKGELDFMSMPAELFMRRA
                     NTPRFQQQFNRFAYFRPAYNYLGWNLRKAMFSDKETRQALTMLLDRDLIRDEIYYGQA
                     EVIAGNFMPGTPECNSALKPLPFDPEKAQQLLNEAGWVFNRSTSLLERNGIPFRFEAA
                     TTNQNPVAEKVLTLYKEELARIGIDLTIRLMEWASLLERVDKREFDAILMGWSMPPDP
                     DPYQVWHSSQADHGSNYIGFVNPEADRLVEEARLSFDRQERIRLYHRFQEILHEEQPY
                     LFLLAPKVLAAGDKRIQGIRIYPFGIDQREWFVPRTMQRFTNTAGVTPP"
     gene            11788..12783
                     /locus_tag="PKN92_02810"
     CDS             11788..12783
                     /locus_tag="PKN92_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ47754.1"
                     /translation="MRAYIIRRLLLIIPTFIGISLLTFMLIQLAPGSPIAFKLRGMDG
                     SMKADAATQEIIEQTKALYGLDKPLPVQYLLWLGRMARFDFGNSMKDQRPVIEKIGEA
                     LPITLLFNVLSLILIYMIAIPLGLFSSTHSGTWQDSTLTFSLFLLYSMPAFWVAMLLV
                     LFLGGGEYLNLFPIQGLHSADADSYSPLMRLLDLLWHMALPLFCMTYGGLAFLSRYMR
                     SGMLDVLRQDYIRTARAYGFSEKTVTWKYALRNALIPIVTLLGGLLPGLIGGSIIIET
                     IFSIPGMGRLAFEAVLSRDYPLIMGDVTIVALLTLAGLLLSDILYVFVDPRIKLG"
     gene            12780..13799
                     /locus_tag="PKN92_02815"
     CDS             12780..13799
                     /locus_tag="PKN92_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ47755.1"
                     /translation="MNNPVTVIETKKRKSLVWRQFTKNKPALAGLILAIAMGLVGLFA
                     PFLAGDIPILMKQNGHLYILPNLFTYQGLQEQNLYQNFDRWEPGAGEFAWRPPVPHGP
                     LRQSLRERLEPPSRKHLLGTDDRGRDVLSRIIWGTRISMSVGFIAEGIAVLLGVILGA
                     LAGYYGGKVDMLVLRLVEIWICVPSFFLVITVMAFLDPSIINIMVVIGLTGWPGIARL
                     VRGEFLKQKQQEYAIAARAAGLNDRRIIFRHLLPNSLSPVFVSATFGVAGAILTESAL
                     SFLGFGVPPPTASWGEILKQSQNYVDFGWWLVAFPGIAIFMTVVSFNLVGDGLRDALD
                     PRLRQ"
     gene            complement(14209..14481)
                     /locus_tag="PKN92_02820"
     CDS             complement(14209..14481)
                     /locus_tag="PKN92_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47756.1"
                     /translation="MDTPEQCVTCRYKRTIRGEARCAKRMMQFCAHEKGLQTLQMVPG
                     ICAVLNPENNCADYRRRNFHRLRTALETNRWLCFLTVVVFAVLIVM"
     gene            complement(14578..16248)
                     /locus_tag="PKN92_02825"
     CDS             complement(14578..16248)
                     /locus_tag="PKN92_02825"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNZ47757.1"
                     /translation="MKSVSLAGSVYNSAFLLAVFCAAFAPAYCDTYHVDIRNETGTAD
                     GSPAHPYTDIQSALDAAHSGDSILVAHGKYAGNITVNNKRVILRGGYAGGSSDQYNSG
                     SSGDFSEQLPKTYVTEIAGESDAPALLLIYDQTSGTEISGLTVSGGSCGVVFDHDYTW
                     PRLENITLSDMVIQGNGTQDQYTTGGGMFVTGSGHSIRNSAIRDNESGFGAGIYLNAD
                     NVTIQDCHIADNQGHGDHGAGIYQGGSAVIERCIISGNRVGVTAGHGWGGGLLLAGTV
                     TMRHNRIYENYSPSGGGGVFADEGAVVTLDHELIYGNRSEDWFRGAAGIYVDGGPGGP
                     SAVEITHCTIAFNQGDGSSGGNGVYVELESSAVLRNCILWGNGGDDFYTDESSTVTAT
                     YTLSEEVIDGTGNFSLDPLFADAAGGDFHLRSTAGRFEPAGGGAGNWVADAADSPAID
                     TGDPGSPFALEPEPNGGRANLGAYGNTPEASKSADASEGEPPAVPHPADADGNGFLKK
                     EDAIGFIEGWQQGTNAMATAIRALYISEKGGSYSYDPALPEPECWVSR"
     gene            16647..17933
                     /locus_tag="PKN92_02830"
     CDS             16647..17933
                     /locus_tag="PKN92_02830"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNZ47758.1"
                     /translation="MGDQSFNAFELAQKQFDAVAEKLGLDAATRELLRNPMREYYFSI
                     PVRMDDGSVQVFRGSRVQHNDARGPNKGGIRFHPQETDNTVKALSMWMTWKCAVVDIP
                     LGGGKGGVVCDPHHLSQQEQERICRGWVRQVARNVGPLADVPAPDVMTSGQHMLWMLD
                     EYETIRGEKYPGFITGKPLSMGGSLGRTEATGFGAIYVLREALKLKNIDIAGCTASVQ
                     GFGNVAQYAIQLFQAYGGIVTCVACWDQTDKVSYTYRKKDGVDLAFLLSITNRYGGID
                     KAQAAANGYEVLPGDAWIEEEVDILMPAAIENQINANTVGRINPSVKVLLECANGPTT
                     LEADAVLKEKNIYVIPDFLTNAGGVTCSYFEQVQCNMNFYWSKEEVLQRLESKMVSAF
                     QAVADLSERESVSMRDAAYMIAIQRVAEACRLRGWV"
     gene            18231..19220
                     /locus_tag="PKN92_02835"
     CDS             18231..19220
                     /locus_tag="PKN92_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNZ47759.1"
                     /translation="MFRGNKPGFTLIELLVVIAIIGILAAILLPALARAREAARRSSC
                     ANNLKQMGVVFKMYAGESSDLFPRIHADQPWGASLPSGCEDGQNRASLAPQMSAVYPE
                     YLTDLQVLLCPSDPERSQKNPLHIIAEAPGQLCAYKGFPSRPDASYLYYGFVIDKASD
                     SDPSFDVGLFGFPSSLISSQMAYLMACLSFMPLLFNGTLGDKNSENDWALNKDLDNPA
                     AYGMFQAMSTPSNVPLGNGDSTTLYRLREGIERFLITDINNPAASAQAQSMLPVMWDV
                     VSATTTASAQFNHLPGGANTLYMDGHVKFNQYPYEFPASKGFASLGSLFSLGE"
     gene            19311..20657
                     /locus_tag="PKN92_02840"
     CDS             19311..20657
                     /locus_tag="PKN92_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ47760.1"
                     /translation="MLLLSAVVLAGGCASLSGNTQDRVLAARNRVAPALVHIRPVKEV
                     FVSGKRTEVLTVGSGFIISADGYIVTNEHVAGESSEVWCILSDNTEHEARVVGVDPYT
                     DVAVLKIEADRPFPYVRWGDSDAIEAGQMVLAMGSPHGLARSVSLGIISVRDRYLENA
                     SGMSSPYNNWIQTDAAINPGNSGGPLVNLKGDVVGINARMLSGAENLGFAIPANIARQ
                     VVTQIIDHGSVRRSALGLVFQEMTSKTDDPSARGVVIADVAPLSASYEAGVRPGDILI
                     RLGNRPMHARHEEELPAVRKAVADLPVGEPVTLVLERGGEEMSFSVVTEEESAYKGRN
                     AEFSEWGFTLVELTPDLARRAQLPGHTGVLVSGCLPGGLAASSGLTPGDIILKLDDTP
                     VASLSDFMNLYKSVQENRKALTMLFVQKGALTRFVLIDQTNSKSSILDKEVLDHVD"
     gene            20647..22032
                     /locus_tag="PKN92_02845"
     CDS             20647..22032
                     /locus_tag="PKN92_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNZ47761.1"
                     /translation="MSIKSFLPFALCLSVLLFSGSGFSFEQEMLQRAYDTISPALCVL
                     TFTQETTDPRSGEQIRQNGFAVALNVSAEGLLLTNGHLQRDNVQSFNFRVTFRIDGKE
                     QSYPAVLLKKPDDLNVSLLRLQSETPLTLPYVRFSTDAGLSLGEPVAILGIMGETLDF
                     NRAIQEDRIISVLDAPRSTYCLGGQLKMGFVTGPVINRRGQVVGITGFDLAVAEGGEF
                     FSRSGVPLVYQASLFEKHIASPPDQTQESVEAEEAWLGVFTQPLKEEFARYWNIPEAG
                     GLIVSTVIPDSPAAAVGIVPGDIIRAFDGHPIRAIHDRDVLAFTQMVKEKAPGSEVEI
                     TLLRNGEELALTATLGHRPRAAQDADEYEDEHLGMVVREITRDLRILLNLGEEVQGVI
                     VRRVVSGSAAQAARIQPGLIIMSMNSATIRDLDDYKEAAQFFKQDKPDEITLFVRAGA
                     QTGFLRLRPRW"
     gene            22049..22642
                     /locus_tag="PKN92_02850"
     CDS             22049..22642
                     /locus_tag="PKN92_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47762.1"
                     /translation="MNGSVRFFAAMLALFSCMLAACHHNPDPDTVLLNAWRLCEKLSY
                     TEAYPLVLESLQAAPGNAVAHYLLGRCFFTQSPPQLTRAKGEYDLALQLLDKNGDLSV
                     LAGKMSEEEFRATLHCDTALALLQTVVEAEKAGMPPRTAVGVMKTALFHVNEGLRFNP
                     ASSFLQELSQTLRHMVDVMNESGTFQEAEPEAPHYFI"
     gene            complement(22646..23599)
                     /locus_tag="PKN92_02855"
     CDS             complement(22646..23599)
                     /locus_tag="PKN92_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47763.1"
                     /translation="MANAVQILLIFLASVLVGLWGCGMLMDTGLLHGFEAEVAVALCI
                     AAAYALVQCLFHGGTLLLKPARGAGVLIAESLSQLSALILLLSLAHVRLPLPAAASSL
                     PALVTPLLYFALFVGLHLFFKLMFFFAAVQGTPVSRAASLFWFLPAGLSVVVMAGAAG
                     SYLLSLENVSLLKQVTPEQIMIGDVTMTARLLPEKKALLFPMKKQPGRTISLFFALPP
                     ELKREVPPLFVRIRSTSESADTGKALFRAAPSRTTRYRLAPVDGAWNAISFDSALLAP
                     DTASILVDWTEKDPADWRARLGLKKTPVSSYRLWVADPVIQ"
     gene            complement(23672..24784)
                     /locus_tag="PKN92_02860"
     CDS             complement(23672..24784)
                     /locus_tag="PKN92_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ47764.1"
                     /translation="MADTARQQMMAWAYDLFQRMYSEAPYRLSPSGKALPAWHYFIEV
                     TRRCNLRCRMCQYLSYLENVPVAEQKKGELSLDEWKNVVDQTNRLGLITFTGGEPLVR
                     SDFFDLLAYASVRRRTHFISNATMLKEAAVHSCVELAPARLGGKGLNALGVSLEAPDE
                     LHDEIRHQKGAFEKSTDGVRLLTEARKKAGKTCPLIHITTVIQQANVHVLAEMPALAA
                     SLGADSLNLVTETRLHDLPGLGEEPPGKWRASDLEWPRVDRTALEKALAETGEAARRH
                     NIELRLPRMPREELLKYYDGGIDARQYTCRSPWNTLIIGRTGDVFPCWLQKIGNVREE
                     KLATMWNSAQTRAFRRACRNCLFPVCPGCCFIEYKK"
     gene            complement(24869..25651)
                     /gene="truA"
                     /locus_tag="PKN92_02865"
     CDS             complement(24869..25651)
                     /gene="truA"
                     /locus_tag="PKN92_02865"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNZ47765.1"
                     /translation="MDTTCLKATVRYQGTRFCGWQRQDNGRTVQGEIETALSRIADRP
                     IAVQGAGRTDAGVHALGQVFSCLWQGSPPQRLAHGLCKMLAPEIRIESLEPVRDDFNA
                     RFDAKGKRYVYSFSLNRHADPLQSLFVWHVPYPVDLDLVRQGLSIITGRHDFAGFQSQ
                     GSQSRTTVRTLVSARLYPGAWCGPADAKNTWTLEFIGDGFLYKMVRNLCGTLIGVGRG
                     RFPLSFIEDSLSRGAPFLGHCAPPHGLALAEVYYEDLPGNTH"
     gene            25811..26437
                     /locus_tag="PKN92_02870"
     CDS             25811..26437
                     /locus_tag="PKN92_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HNZ47766.1"
                     /translation="MKLKKTIFCLLLILLVPVPAGFAQYVAEGALIGGAFGTGTGALI
                     GSYSGRTGEGALIGAGIGALAGAAIGSASRPHYYRSTAPYPYIMPVRHHAYYAPRPYY
                     HIRPPLRPVVYHSARPVTWYRPAGVPVRTAVAVPVRRVAVPAVQAPIPRAVPLVDTHP
                     IATLPAPGAAAAPAARSTVPAPDTTQRFTVRTVTTPSGERYEERVALP"
     gene            26457..26771
                     /locus_tag="PKN92_02875"
     CDS             26457..26771
                     /locus_tag="PKN92_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47767.1"
                     /translation="MKARTLWRLTGLLCLAGAFVLVLFSDPLLRGDADYRFIMIFWGS
                     LLCLLGAVVYIAFLDIHFTRLVYKKQERALFRQTFLGERHKEEAHEKADESGSSVPPE
                     EA"
     gene            complement(26797..28023)
                     /gene="ispG"
                     /locus_tag="PKN92_02880"
                     /gene_synonym="gcpE"
     CDS             complement(26797..28023)
                     /gene="ispG"
                     /locus_tag="PKN92_02880"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNZ47768.1"
                     /translation="MPGLTHRVLVDSVLIGGGAPVVVQAMTNTNTEDALSTAEQCAAL
                     AAAGAELVRITVNTPRAAGAVPEIVLRLRDAGYPVPLIGDFHYNGHKLLQKHPQCAEA
                     LAKYRINPGNVGQGDARAIHFSEICAIARDLKKPVRIGVNAGSLDQALLEKLQRENLK
                     QQHPGAAQEVFNRCMVLSTLQSAEQALEAGLSENQIILSCKSSQPVDLITVYRSLAEK
                     TRQPLHLGLTEAGLGAKGLIWSAAAMGILLEAGIGDTIRVSLTPEPGGDRCGEVYAAQ
                     ELLQALGLRQFSPSVTACPGCGRTSGDAFQRLAADTQAYIRMRLPLWREQYPGVETLK
                     VAVMGCVVNGPGESRAAHIGISLPGSNEAPLCPVYMDGEKQCSLQGSMEEITRSFLGL
                     LEDYVIRRFGTPESAD"
     gene            complement(28060..29328)
                     /locus_tag="PKN92_02885"
     CDS             complement(28060..29328)
                     /locus_tag="PKN92_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNZ47769.1"
                     /translation="MFELIQANQRRSRWLLAGMFLVLLGVGFTIGALMLPTPEQALAG
                     GIIGMGVAFMIWALQALIAWSSGDRLLMAVAGARQIQKADHPQLFNVVEEMTLAARLP
                     AVPQVYIIEDMSLNAFATGRKPEKAAVAVTAGLLSKLNRDQLQGVIAHEIAHIANRDT
                     EFMTFAGIMLGSIILITELFHRSIWYATRTTGSMRRYSSNRSGKDGNAALLVLLVISM
                     VLAVLAPLLAQILYFACSRRREYLADAGGAVFTRYPEGLASALEVIAGNPGDKETTSR
                     AMAPMYIINPLQSARSFNSLFSTHPPVDERIRILRNMSGGTAYRDYAAAWEKVSGSKA
                     VDMPASAMAFTAAQSAVPAAQDSAAAAASSVPADQDARQRHRETAEMLRRMHHYRLIT
                     CSCGLKAKIPPQYPHAAVKCPRCGKMHRIA"
     gene            complement(29383..29961)
                     /locus_tag="PKN92_02890"
     CDS             complement(29383..29961)
                     /locus_tag="PKN92_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNZ47770.1"
                     /translation="MTGTIILIAAGAVLLIFVWWMIAIFNGLVRLRNAVKNAWAQIDV
                     QLKRRHDLIPNLVETAKGYMKHERETLESVIKARNQAAEASKNSAGASAQGALESGLS
                     GALRNFMVVVERYPDLKADTLFSNLQEELASTENRISFARQAYNDAVMMMNNKVEMFP
                     GNIVAGMFNFSKESFFEIEVEAERAVPEVSFS"
     gene            complement(29958..30395)
                     /locus_tag="PKN92_02895"
     CDS             complement(29958..30395)
                     /locus_tag="PKN92_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNZ47771.1"
                     /translation="MKCPACFEILLTLEYQDVEVDYCAACQGVWLDAGELELLLGEAR
                     LSKAFLTAGSKAVAAGEKKRLCPLCDDAMDKEVTGGAKPIVYDRCPKGHGLWFDHGEL
                     LAVIQSDCTEGDVRTVSLWLQEMFPPSTEQYGVENQPDKGVSS"
BASE COUNT         6930 a         8769 c         8299 g         7026 t
ORIGIN      
        1 cctacagcac ccgatatccg gagctgcccg ccattctggc agaaaaccct aaagcgccct
       61 gcggtaatct catcgcccgc aatatctgtt taggcggaac ctgggatgaa gtggaagaca
      121 aagcgcggga tcttctccgt tttgaagaca acctgctgaa tgatccctcc tgcttcgctg
      181 atgcgaaagc gggtgattac agcctgccgc ccgattcacc ggcacgcgcc aaaggcatcc
      241 gggaaattcc ggtgaacgaa atgggtacgt acagccatcc ggactgcgcc ttctgattcg
      301 tcttcagctg taaataatca gagcgcaggt gatcgcccag agcagaacag cgacacaggt
      361 cggcaggtct ttcagaagca cctgactggg gtcgccggtt tcgttttgtt ccaccagcca
      421 gaggtagcgt gccagtccgt aaatcacaaa gggcagggtc atatacagct tgtctgtgcc
      481 gatgcgggac accacttcgg ggctgcaggt gtagatggtg aaggtgatca tggcaccgcc
      541 tgccacaaca atcagcattt tatcgataaa gtcgatgctg taatggtgca gtacttcccg
      601 atgatcggcg gcatcctctt ccagcagggc aatctcgccc cggcgtttgc ccagaccgag
      661 gaagagcgcc agaaaaagcg tgcagacaat gagccaattg gaaaaaacca cgtcgatgac
      721 cacggcaccg gcaatggcgc gaacaacaaa gcccagcgcc acggcaatca catccacaat
      781 aaacaggtct ttcaggaaaa agctgtagga tgccgtcagc gccagatagg tcagcagcga
      841 taacagaaat gcccagttca gatgaaaggc gagtgccaga gacagtacca gcagtgcggc
      901 ggaaaagagc gaagcaggaa caggtgccac agcgccggat gccagagggc gcatgcgttt
      961 ggtcggatgg ttccggtcac tttcccggtc gcgcaggtcg ttcacgatat aggtggcgct
     1021 ggaagccagg caaaaggcaa cagtggcgag gaggcttcgc accgctgctt ccggattgag
     1081 cagctgctgc gcaaaaacca atggcataag aaccagagcg ttttttgtcc actgataaac
     1141 acgcagcagg cgtatccagt cagaaaagcg gcccataatc tgttctttct tatatgctca
     1201 ttttcttgaa ctgccactca gggatatttc tgatgatcag catgatccag cgccagaaaa
     1261 agggcacata aatagtattg gctccccgaa gaatggcttt cacaatcact ttgcccgccc
     1321 cttcagggga tgccaccagc ggaccgggca acggcaaacc gaaggtcatt gccgtatcga
     1381 taaatccggg aagaatggtg gtaacctgaa cgttggcagt atacaggcgg ttgcgcagac
     1441 cgcttaaata ggcggtcagt ccggcttttg cggcaccgta gatatagttg gacttgcggc
     1501 cccgctcgcc cgccacggaa ctgatggcgg caataaatcc gctgccgtgt ttttcgaagg
     1561 cggcggcaaa cttttccaga atggaaatgg cacccagcaa attgacttcc atcgtccggc
     1621 gcatcgctgc cgtgtccatc tgtgccagct gctggtccgc catataaccc gcacagtata
     1681 cgattccgcc gggcagattc ccgaagcttt cactgcattg atccagaaag ctgctgtgcc
     1741 cggcgttgtc cagggcatca aaaaagcgca catgacacgg cacttcgtgg cggatagtaa
     1801 gatcttttgc aatacggcgc agctcctcaa catcccgccc ggcgagaagg atggaaaagc
     1861 cctgtctcgc aaactgatcg gcaacagcgc gcgccacgga agaaagcgca ccgacaatca
     1921 caacggtttt tctggaaata gctgtcatga ccgctgtcct ttctatacca ggtgcaggcg
     1981 ttttgcctga tccgatgaga ggagctgctg cgggtccagt tcccggcgca gactccggaa
     2041 cctttccagc gcaggataca tcgatgcaaa tgtggcggca tcggttacgg catcttttgc
     2101 cagataaagc ctgccgccat gatccagcag aatgcggtca agttcttttg tgaagtcttc
     2161 aattcctttt ttgcacggaa tatccagtgc cagcgtatag ccgggcatgg gataggagag
     2221 cagacccgga tttgaagcgc cgaagcattt cagcacagcc agaaaagaag cccgtttttt
     2281 ctcggacagc aattcaagca tcttcaccag acccgtgcgc ccttcgtagg gaaaagccgc
     2341 ctgatactgg ataaaaccgc gccgcccgta catacggttc cagtcgtgaa ttccgtcgag
     2401 gggataaaaa tatttgtcgt aattgataat agcctgctct ttatcccggt tcagcagata
     2461 aaaaagcgtg ttgaacgcct tgatgctcca gttattcagc acaaagcccg ggaagtcaac
     2521 aggtactgcc aggggcgcct ttttcggaag ccggaaggga ttgcgctcaa gacgcacagg
     2581 cagatcgcga aggcgggcat gagcgccctg catcagaacc gacctgccaa gctgctggcg
     2641 tcgcgcaaga caatcgatcc acgcgacaga gtaaagataa tcctgatccg tagccgccat
     2701 tttttccagc acgtcatcca gatgcgcaca gcgccggtaa tccaccttta cataggctgt
     2761 ctccaccgca cggagctgaa ggcgcacttt taaaataaca cctgtcagcc cgacaccgcc
     2821 cgctgtcgcc cagaagagat cgctgttttc tgaagcggaa caggtcagca cgcctttgga
     2881 cggcacccac aggtcgaagg aatcaacaaa ctggcagaag gtgccgtcga catgatgatt
     2941 ttttccgtgc acatcgttgg cgatggcacc gccgactgtg acaaatttgg tgcccggcgt
     3001 taccggcaaa aaccagcccc gggagacaaa tgcgtcaaga atttccgcca gagtaacccc
     3061 gccctcgcat tcaagaatgc cgtgctcttc atcaaaatac agcatgcggt tcatacggga
     3121 catgtccagc gccagccctg ctgaattcac cgccgtatcg ccatagctgc gcccgagccc
     3181 ccgggcaata aaagacatat ctccggattc ttccagaaaa ttcttcagct ccgacgtccg
     3241 ctccggacgg aaaacagagc actttttcgg gtggtaacgc ccccagccgg caagatttat
     3301 tgaggcagta ttgagaagca cagaacgtcc ttccatccgt ggaaaaccag acacagagca
     3361 ctctgacagg aaaagtcagg cagttcagta taccgaaaaa gcgccggagg taacaaaggt
     3421 cataaagaga aaagagaaaa tacgccgtct ttttctgtaa aggccgcagc gctatccggg
     3481 ctcacgcagg gaagcgttat tgggcagccc tccgtgcata gtgtacaata agtgaaagac
     3541 tggctttgga tttcatcgaa caggaagaca gccggcacta ctcttacggt atcggtcata
     3601 tgcagcaatt ttcttttcag cagcaaggtg ctttttttca tacgccccgc atcacttggg
     3661 cggtgcagat gcttattctg gtgaatgccg cctgctttgc cctgcagctc attgcctatc
     3721 cttttgaaac ggggctcgct tcgtcctggc tccggctgga tatgtggttc ggctttcagc
     3781 cggcagcttt tctgcacggc ttcctgtgga tgcccttcac ctatcagttt ctccacggcg
     3841 ggctcctcca tctctttatg aacatgctgt ggcttttcgt tttcgggccc gatgtggagc
     3901 gtctgctggg gtcgcgtcag ttcctgtttt tttatatcgc ctgcggtgca ttgggcgtac
     3961 ttgcttcgct ggtgccttat ctggctaccg gacacggagc gctgattatc ggggcgagcg
     4021 gctcgaccat gggtgtgctg gttgcctttg ccatgatcga tccgcagcgc cagtttctgc
     4081 tgttcccgct gcctgttccc gtaacggccg tatggctcgt tattcttgtg gtcattttca
     4141 atatcctcat ggcacgatcc ggcaattcag gtatttccgt tgccacccac ttcggcggga
     4201 tgcttgcggg cggtgcgctc atgaaaggca ttccccgata tcacagctgg cggcgaagca
     4261 gaagacgctg acccggacaa acaaagacac agtttcctga acgtactcaa aggattttcc
     4321 ggatgcccgg cttaccgaaa attatatttt ttgcaatcgt cttttatatc cttcatgcca
     4381 cagggctgct cggttttttt cgtcgtcttt tcgatgaggt gaaaaaaagc atggcgcagg
     4441 cggatgcacc cggaaaaagt gccggtgatg cggaagtatg ttaccggctc ctcggcgtga
     4501 ccccctccgc taaatgggaa gaaatagaga aagcctaccg ctccaaagca aaggtgcatc
     4561 atcctgatct ggggggcgat gaagatgcca tgcgcgcact caacgaagcg tacaactata
     4621 tcaagaagct gaggaaaggc tgagtgcatg gctccgcctg tacaccctat tgacgctccc
     4681 ggtggacatg aatcttcatc ggagggccaa tcggcggata cctgcgacag aaccggaacc
     4741 atgaatcccc ggctccggcg catcctcctg gttctttgcc tcctcattgc agcaggcatc
     4801 cgctgctcgc tcttcctcga aagccgtagc ggcccggatt atgcgcaccc tcttcatgat
     4861 gctgaattca gggactactg ggcgcgggct atcgttaccg gcgattggac gccgccgccg
     4921 ggacgggaac agccttttgc cgactggctg ccctacccgc atgcgcccgg ctattcctgg
     4981 ctgttgtcgg gcatctaccg cattttcgga ctcggatacg atgcgccgcg gctgatacag
     5041 atgttccttg gtctgatcag catcctgctc ctctacgaca ttgcacgacg gctttacaac
     5101 gataaagccg gtatcgccgc cgccgccctg atgagccttt atttcgtttt tcccttctac
     5161 gaaatggatc tggggcaaac cagccttatc gtttttttgt tacttctttt aacgctgttt
     5221 ggcagcaaag ctgttaaaaa acagcacctg ttatttttaa caatcaatgc tttgctattc
     5281 gggatacttc ttcttgttag aaatgaattt ttgccctttg gaattatttt tctgggcgtg
     5341 catctgttta gttatttaaa aagcaagaac tggaaaaaga tcttggcagg aaccactctc
     5401 tggtgcctgt ttagcattat tcccgtcacg ccggtttctg tatacaacta tcagcatgcg
     5461 caggtattgc ctattgttgg tggcgaagga acctatgttt tctattgttc aaataatcaa
     5521 tgggcaactg gaacaaaccc cgactcccct gatttgcgtg ctctggaaaa aaatgacgaa
     5581 tggacttttt tctcttttcc tgcaatacag gaggcttgcg ctcgcgctca gaatattacg
     5641 ccgctctcct atagagaact ccaaaaacat tttaaacagc tcaatcgaaa atacatagca
     5701 acagaaaaaa agcgtctctt ttggctaatg cttaaaaagg ctgctctctt ctgggggccc
     5761 gttgaggttg acgagaacaa agtggttgcc tgtgaaaagg actattttgc cgtcctccgt
     5821 tatctgccgg gatttccacc ggcgatggcg ctgttcctgt tgggagccgt actccttttc
     5881 attcgggcaa tccgcaaaga aaaaaactgc acaaaggaaa acttggcggc atggacgctc
     5941 cttctgctgg caatggcagg atacagcgga atattcgttc ttttttatgt cacggcgcgg
     6001 tatcgggttc ccgtcattcc tttcatgctg ctcttcggtg ctgtggcggt ttctgactgg
     6061 tttgaatggc tgcgacggaa agcgtttaaa aaagccgctg tcggacttac cgccttcctc
     6121 ctcctcacag cactgctcag cagacccctt gttccattta cgcccgacac cgagagatgg
     6181 ctggatgaac ggcggcggct ctgggtgaag accgacagga tcgatgaggg catcgccgat
     6241 caggaagcgt ggaatatacg ccatccgcag agcccgggcg gacagtatca tcttgctctc
     6301 cttctgattg aaaaagggga ctacgagaaa gcgctgaatt gcctgcagcg aaccattgcg
     6361 ctcgattccg gctacgatgc cgcctattac aatcagggca ttgtgctgct caaattgaac
     6421 agaccggagg aagctctcga atctttcaga atccgtctgg atcgtgtgcc gaacgatgaa
     6481 aaagcctggt tcggtctggc gcaggcacag cagatgctgg gagataccgg attgcagcgt
     6541 gaagcgctgg atcatgccct gtctctgaag ccaggttatc cggaagcgct cgtggaccgc
     6601 agcacactct tcctgcagga gggcagccgg gaccgggcgc aggctctgct ggaaaaagcg
     6661 ctggaagccg caccgggcta tgccatggcg gaatttaacc tcgggctcct cctcgccggt
     6721 gaaggaagct acagcaaagc cctttccttt tttgaatccg cactcacgca cgcacccgac
     6781 cgcgccgatt ttcattacaa taccgggctg gcgctgattc agctgaagca atatgaagag
     6841 gcggtccggc attttcatga ggcgctgcgt ctgcggcccg actatcccga agtctattcc
     6901 aatctcggtc tttccgaagc cagacgccat cacgaagaag aagcaaaaca cgccttcgac
     6961 cgcgcctttg aactgctccc cgaggacagc gacactcttt acaattacgg atgctcgctg
     7021 aattctcttg gcgacaccgc aggcgcgcgc cgctattttg aagccgtgct ggaacaggca
     7081 cccgatcatg gaaaagcact cttcgaactg gcgctgctgg acattaaaga aaaagcactg
     7141 gacaatgccc ttatccgtct caaccgggca cgtaccattc tgtcaggcga cccggaggtg
     7201 tgctacaatc tcgggctcat ccggcagcaa cagtatgcat ccgatgaggc acagcgtctc
     7261 tttctggaaa cgttgcagct ggatcccggc cacttcggcg ccaacaggag cctcggtacc
     7321 cttctccgca gcgaagggcg ttccgccgat gcgctccctt acctgaaggc ggcgctcgaa
     7381 gcttccgaag cggcagacga cagcgcactg cattacgaat acggtgtggc actctacgac
     7441 ctgcgccatt ttgatgaagc cggacaggcg ctgaacgaag cggcacagct ggcgcccgga
     7501 tccgcagcta ttcgaaccca ccaggcgctg gtttccattg cccggcataa ctttggggac
     7561 gctgtagccc gtttccggga agccgttgcg ctggatccgg acaacctgct gctccgctac
     7621 cggctcggcc ttgccgaact cgaagaggga gacgccgcat ctgccgccga tacattggca
     7681 ccgcttctga aacagctgcc ccaccggaaa gaccttttct gcgattatgg aagagccctt
     7741 gccgctctgt caaagcacga cgaggcatta caggcatatc gcgtcgcctg gctgctcgat
     7801 cccgcctatc tgattgccat aaacgccctt gctttttcgc tgtccatgtc aggcagaccc
     7861 gccgaggcac tggaatggct tgatctggcg cttgcgcagg atccggagaa tcaggatgcc
     7921 cttttcaata ccggccatgc cctccatctt ctcggaaaac tggaagcggc cgaccgcctg
     7981 ttccttgcgc tcctgcgcct gaatccccat cacagcaccg ccctcctcaa ccggggactg
     8041 attctggaaa tgcagcagcg ccccgaggag gcactggcac agtttgaggc tgcattaaaa
     8101 gaaaataacg gccttgcgct ggcacatcat caccttgccg ggctgctccg ccggaaaggc
     8161 gaccgggaac aggcgctcag acattaccgg caggcgcggg aattggatcc cggcaatcag
     8221 agcaccgccg tcaatcttgg tttcacgctc gtggagctcg ggcgtcttgc cgaagcgaaa
     8281 gagctctttc tgcaggcgca ggaacaggca cccgatgatg ccgaagccta tatcgggcag
     8341 ggggatgtgc ttctggcgga aaataacctc aacgaagccg aagcggcgta ccggcatgcg
     8401 ctgcgtctca gtcctgacaa tgccgttgcc ctcaaacagt acggattcct gctggtgcgt
     8461 cagggaaaaa gtgctgaagc cctcgaaaaa tttgaggcgg cacgcgcagc gctgccttat
     8521 gatgcggggc tctactgcgg actgggtgat gtttcttttg cccgcggtga actggaacgt
     8581 gccgaaagct attatcagaa agcagtggag ctgtatcccg atttcgctct cgcctggaac
     8641 aactacggtg atcttctcgc aaggaagggg cagacgcagg aagcgatcag tgcctatctc
     8701 agggcgcgcg ctctcgaacc ggaaaacacg agcgtgcttc tgaatctggg gcgcctctat
     8761 gcccgggaaa aagaagcagc cccggctgtg gaagtgctgg aaacagcgct ccgtcttgag
     8821 cccgataatc tccgtgtgcg ggcacttctg ggcgacatgc gcatgctcac aggcgatgcg
     8881 ccttccgcta tccgggaata ccagcgcatt ctgctggaac ggcctgactg gcaggagatc
     8941 cgggacaaac tcgataccgc acgggctcag gcggcagaaa actgaattgc ttctgcggtt
     9001 aaaagagacg cccaacagaa tcagacacgc cgtccctgta tgggaacaca cggctgaacc
     9061 tgatataata ccctttattc tccgcaggaa cagcctggtg gattaaatag tgccgggcgc
     9121 tgcggcacag ctgtctctgc tgcagaggcg gtgtgtttgt gtttgatttt gctttcacct
     9181 atataaagga tttcatcatg aacttttcta ttgccgatgc tgaagtattt gttcctgatc
     9241 aggcgaccat gcccgaagcg ctcagccggg tgacccatct tgctgtgggt gcccatcagg
     9301 acgatctcga aatcatggcg gctgacggca ttctcaaatg ctttcagcag agcgacaaat
     9361 ggtttgccgg tgttgtcgtc accaacggcg gcggttcgcc ccggacggga ctctatgccc
     9421 atctcaatga tgatgagatg atggctgtcc ggcgtatcga gcagaagaaa gccgccatca
     9481 tcggcgacta cggtgcacag tttcttctca accatcccag cagcgccgtg aaagatggcg
     9541 ccaatcaggc ggtagtggat gaccttgtcg ctgtgctgaa agcaacccgc cctgaagtca
     9601 tttacacaca caacccagcc gataaacacg acacccatgt gggtgtgctg ctgaaactga
     9661 ttgccgcact gcgccgcctc ccgaaggaag aacgaccgaa acatctttac ggctgcgaag
     9721 tctggcgtga tctggattgg ctctgtgatg aagataaagt cgcttttgat gtttccgccc
     9781 atgaaaatct gcaggcggcg ctcgtcggtg tgtttgattc tcaggtggca ggaggaaaac
     9841 gctatgacct cgccacactc ggcaggcgtc gtgcccatgc cacttacagc gcatcacacg
     9901 ctgtcgacac tgccgtcgca attatttacg ctatagacct cagtccgctt cttactgatg
     9961 attcgctgac cctggaagcg ctggtcgaag cgcatatgaa acgcttcaac gaggatgtga
    10021 ccggcagaat ccgcaaaatg tcctgacggg cttccgggta catagtgtat cccgccttca
    10081 actgcacagg aacaggctta tgaaacagta catattcgtg tgcttgcttt tcgtggcatt
    10141 gctttttgca ggatgcggcg ataccggacg aagcacttcc ggtcccggcg atatcctcac
    10201 ggaatacatg ccggatgcga tgccggagca gggtgacgtg ctccggctgc tgctcccggc
    10261 ggaaatgcct catctgaatc ccctcaccag cacggattat tacgccagca cagtactgga
    10321 atggatcttc gacacgctcc tcgaccggga ccccgcaacg ctcgaaagca ggcccgccat
    10381 cgcggcacgc tgggaagtgt ccgaggatca tcttgcctac actttttacc tccgggatga
    10441 tattgttttt tccgatggta cgccgctgac agcgcaggac gtcaaattct cttttgacac
    10501 agtcatggat ccggccgtag atgcacccaa cctgcgcaac tatttcatcg atatcaaaga
    10561 atgcattgcc gttgatgcat acacggtacg cttcacctgc gacaagccct attatcagca
    10621 tctgatcatg cttggcgggc tcagcatcat gccaaagcat atttatggcg agggggattt
    10681 caaccggcac ccgaacaacc ggaagcccgt cggttccggc atgtatactc tcgaagcatg
    10741 ggacacggga cagcgcctcc tcctgcagcg gaacccccgc taccgtggcg ccctcgaggc
    10801 aggaaggccc tggttcgacc gcatcgaata cgccatcatc cccgatgaca atacggcctt
    10861 tcaggtgctc gccaaagggg agctcgattt catgagtatg cctgccgaac tgttcatgcg
    10921 ccgtgccaat accccccgct ttcagcagca gttcaaccgt tttgcctatt tcagacccgc
    10981 ttataactac ctcggctgga atctgcgcaa agccatgttc agcgacaaag agacacgtca
    11041 ggcgctgacc atgctgcttg accgggacct gatccgggac gaaatctatt acgggcaggc
    11101 ggaagttatc gccggcaatt tcatgcccgg cacgccggaa tgcaacagcg cattgaaacc
    11161 gctccccttc gatccggaaa aagcgcagca gctgctgaac gaagccggat gggtttttaa
    11221 caggagcacc agcctgctgg aaagaaacgg aatacccttc cgcttcgaag cggcaacgac
    11281 caatcagaat cccgtggcgg aaaaggtact gacactgtac aaggaagaac tggcgcgtat
    11341 cggcatcgac ctcaccataa ggctcatgga atgggcatca ctcctcgagc gggtcgacaa
    11401 acgtgagttt gatgccatcc tcatgggatg gagtatgccg cccgaccccg acccctatca
    11461 ggtatggcat tcctctcagg cagaccacgg ctccaactat atcggctttg tcaatcctga
    11521 agcggaccgg ctcgtcgaag aggcacgtct ttccttcgac cggcaggagc gtatccgcct
    11581 ctaccatcgc tttcaggaaa tccttcacga ggaacagccc tatctctttc ttctggcgcc
    11641 gaaagtgctg gcagcaggtg ataaacgcat tcagggtata cgcatctatc ccttcggcat
    11701 cgatcagcgt gaatggtttg tgccccggac gatgcagcgt ttcaccaata ccgccggcgt
    11761 cacgccgccc tgatcagggg aaatgccatg cgtgcgtata ttatccgacg actgctcctc
    11821 atcatcccta ctttcatcgg gatcagcctg ctgactttta tgctgatcca gcttgccccg
    11881 ggaagtccta ttgctttcaa gctccgcggg atggatggct ccatgaaggc tgatgcggca
    11941 acgcaggaaa ttatcgagca gaccaaggcg ctctacggac tggataagcc gctgcctgtg
    12001 cagtatctcc tttggctggg gcgtatggcg cgctttgatt tcggaaacag catgaaagat
    12061 cagcggcctg tcattgaaaa aatcggcgag gcgctgccta tcacgctgct ctttaacgtg
    12121 ctgtccctga ttctcattta tatgatcgcc attcccctcg ggcttttttc ttccacccac
    12181 agcggcacct ggcaggacag cacgctcacc ttctccctct tcctgctcta cagcatgccc
    12241 gctttctggg tcgccatgct gcttgtgctc tttctgggcg gcggcgagta cctgaatctt
    12301 tttcccattc agggactgca ctccgccgat gccgacagtt attcaccgct catgcgctta
    12361 ttggatctcc tctggcatat ggcgctgcct ctcttctgca tgacctatgg agggcttgct
    12421 tttctctccc gctatatgcg ttccggcatg ctggacgtgc tccggcagga ttacatacgc
    12481 accgcccggg cttatggttt ttcagaaaaa accgtcacct ggaaatatgc attgcgcaac
    12541 gcactcattc ccattgtaac gctgctgggc ggattactcc ccggactgat cggcggaagc
    12601 atcatcatag aaacgatttt ctccataccg ggcatgggtc gcctcgcctt cgaagccgtg
    12661 ctcagccggg attatcccct gattatgggt gatgtcacta ttgtggcgct cctgaccctc
    12721 gccggacttc tcctcagcga tatcttgtat gtctttgtcg atcccaggat caaactcgga
    12781 tgaataaccc tgtcactgta atagaaacga aaaagcggaa aagcctcgtt tggaggcagt
    12841 tcacgaaaaa caagcccgct ctggcggggc tgatcctcgc catcgccatg ggactggtcg
    12901 gactttttgc gccttttctc gcaggggata tccctatcct gatgaaacag aatgggcatc
    12961 tgtatattct gccgaatctg ttcacctatc agggattgca ggaacagaat ctctatcaga
    13021 acttcgaccg ctgggagccc ggcgccggag aatttgcctg gcgcccgcca gtgccgcacg
    13081 gaccgctgcg ccagagcctc cgcgaacggc ttgagcctcc ttcccgcaaa catcttctcg
    13141 gcaccgatga ccggggcaga gacgtgctta gccgcatcat ctggggcacc cgcatttcca
    13201 tgtccgtggg ctttatcgcc gaaggcatcg ccgttcttct gggcgtgatc ctcggtgcgc
    13261 tggcaggcta ttacggcggt aaagtggata tgctcgtgct gcgcctggtg gaaatttgga
    13321 tctgtgtccc ttcttttttt ctggtcatca ccgttatggc gttcctcgat cccagtatta
    13381 tcaatattat ggtcgtcatc gggctcaccg gctggcccgg cattgcccgg ctggtgcgcg
    13441 gcgaatttct taaacaaaag cagcaggaat atgctattgc cgcccgggcg gcgggactta
    13501 atgaccgccg tatcatcttc cgccatctcc tgcccaattc gctgagcccg gtttttgtca
    13561 gcgcgacttt cggtgtagca ggggctatcc tcaccgaatc cgccctgagc tttctcggct
    13621 tcggcgttcc gccgcccact gccagctggg gcgaaattct gaaacagagc cagaactatg
    13681 tcgacttcgg ctggtggctc gttgctttcc ccgggattgc gatcttcatg accgtcgtct
    13741 ctttcaatct tgtgggggac ggtctccgcg acgcgctgga tccacggctt cgtcagtagt
    13801 gacgaggcgg aagaacgctt cggctctgaa gatattgcca tcaactccgt cgggaaagtg
    13861 ctgctttcgt ttaacagagc cgcattccac acgagaatca gtggacagag tgggcacagt
    13921 ggacagagtg gacagagtgg acggagtgga cggagtggac agagtggatg gagtgggctt
    13981 cttcttcctg ccgtcatgcc acccatttcc cgtcattcct gccatttccc gtcattcctg
    14041 ccatttcccg tcattcctgc gaaggcagga atcttaacca gggtaaggac ggctttcgtt
    14101 taacagagct gcattccaca cgagaatcag tggacagagt gggcagagtg gacttcttct
    14161 tctgaggccg cctttctttg aatcatatcg tcccctgccg tgctgccgct acatgacaat
    14221 cagaacagca aacacaacaa ctgtcagaaa gcacagccat cggttcgtct caagagcagt
    14281 gcgcagacga tggaagttcc gtcggcgata atcagcgcag ttattttccg gattcagcac
    14341 ggcgcagatt cccggaacca tctgcaaagt ctgcagtccc ttctcgtgcg cacagaactg
    14401 catcatcctt tttgcgcatc gtgcctcccc cctgatggtt ctcttatacc ggcatgttac
    14461 gcactgttca ggagtatcca tggaactgtt ctccgttgct gccatcaccg tctgcccttt
    14521 gcgccgcttc ctgtgctttg catctgataa ccgtaataaa gcaggtgcaa gaccggttta
    14581 tctgctgacc cagcactcag gttcgggcaa tgcgggatca taactatagg atccgccttt
    14641 ttccgatata taaagcgcac gtatcgctgt cgccattgca tttgttccct gctgccaccc
    14701 ctcaataaag cctatcgcat cttccttctt caggaaaccg ttgccgtcag catcagcggg
    14761 atgaggcacc gccggcggtt ctccttcgga tgcatcagca cttttgcttg cttccggcgt
    14821 attgccatag gcccccagat tcgcgcgccc cccgttgggt tccggttcca gagcgaaggg
    14881 tgagccggga tcacccgtat caatggcggg gctgtctgca gcatcggcca cccaattccc
    14941 tgcaccgcct ccggcgggtt cgaaacggcc tgccgtggag cgaagatgga aatctccgcc
    15001 ggcggcatca gcaaataaag gatcaagaga aaaattgcct gttccgtcta tcacctcttc
    15061 actgagtgta taagtggctg taacagtgga actttcgtcc gtgtaaaagt catcgccgcc
    15121 gttaccccaa aggatacagt tgcgcagcac agcggaagac tccagttcca cgtagacgcc
    15181 gttgccgccg cttgatccgt ccccctgatt aaaggctatg gtgcagtgtg tgatttcgac
    15241 agcagaaggg ccgcccgggc ctccgtccac ataaataccg gcagccccgc gaaaccagtc
    15301 ttcggagcgg ttcccgtaaa tgagttcatg atccagcgtt acaaccgccc cttcgtcggc
    15361 aaaaacgcca ccgccgccgg agggcgaata attttcatag atacggttat gccgcattgt
    15421 cactgttccg gcaagcagca acccgccgcc ccatccatgt ccggcggtta cgcccaccct
    15481 gttgccggaa atgatgcacc gttcaatgac agcgcttccg ccctgataga tacccgcacc
    15541 atggtctccg tgcccctgat tatccgcaat atggcagtcc tgtatagtga cattgtccgc
    15601 attgagataa atgccggcac cgaaacctga ctcgttgtca cggatggcac tgttccggat
    15661 actgtgtccg cttcctgtga cgaacatgcc gccgcctgtg gtatattggt cctgagtgcc
    15721 attgccctga atcaccatat cagagagggt aatattttcc agccgaggcc aggtgtagtc
    15781 atgatcaaac actacaccgc agctgccgcc tgatacggtc aggccgctga tttccgtccc
    15841 ggatgtctga tcgtaaatca gcagcaacgc cggcgcgtct gactcaccgg caatttcagt
    15901 cacatacgtc ttcggcagct gttctgaaaa gtcgccgctg ctgccgctgt tgtactgatc
    15961 agatgatccg cctgcatatc cgccgcgcaa gatcacccgt ttgttgttca cagtgatgtt
    16021 tccggcatat tttccgtggg caaccagaat cgaatcaccg gagtgtgccg catccagtgc
    16081 gctttgtata tccgtgtaag ggtgtgcagg cgagccgtca gcagtacctg tttcattccg
    16141 gatatcgaca tgatacgtgt cgcaataagc cggggcaaag gcagcacaaa agacggcgag
    16201 taaaaaagca ctgttatata cagaaccggc cagagacacg gatttcatcg tgagcgttcc
    16261 tattcgtaac gaagcaacag ttaaagcacc ataaaagacc gtctgcatta tacttcagat
    16321 ttcctgaaat ggcaagcatt tttcctgggt gtttcccgta aaaacgcctg tatttccttt
    16381 cgatatccct ttgtgtcggc tcctttgaag aaaatttgcc gagcccgtcc gcattggaat
    16441 aaaatatgga ggcttaaaaa cgatcggggg tgcccggaat ctgaaatcca gtcaatgcac
    16501 gacagtaaaa ctgtcggaga aaaagaccgt ggcgggatcc cgatttttcc tttccagtcc
    16561 ggctcaattc agttcttttc attgcgccct tgcacgaagt caagggttta cacaccaggt
    16621 tcaccagcct cataaggaac agtacgatgg gtgaccaatc ttttaatgcc tttgaacttg
    16681 cccaaaaaca atttgacgcc gtagccgaaa agctgggtct ggatgctgca acccgcgaac
    16741 tgctgcgcaa cccgatgcga gagtactatt tcagcatacc ggtgcgcatg gacgatggtt
    16801 cggttcaggt gttccgcgga tccagagtgc agcataacga tgcgcggggt cccaataaag
    16861 gcggaattcg tttccacccg caggaaacag acaacaccgt caaggcgctt tccatgtgga
    16921 tgacctggaa atgcgctgtt gtggacattc ctctgggcgg cggcaaaggc ggggttgtgt
    16981 gtgatcccca tcatctgtcg cagcaggaac aggaacggat ctgccgcggc tgggtacgcc
    17041 aggttgcccg caatgtgggt cctcttgccg atgtgccggc gcccgatgtc atgacttccg
    17101 gtcagcatat gttgtggatg ctggacgaat acgaaacgat ccgcggagag aaatatcccg
    17161 gattcatcac aggcaagccg ctgagtatgg gcggttctct gggccggaca gaagccaccg
    17221 gcttcggcgc tatctacgtc cttcgtgagg cactgaagct gaagaatatt gacatcgccg
    17281 ggtgtaccgc ctcggtacag ggcttcggca atgtggctca gtacgccatt cagctgtttc
    17341 aggcgtacgg cggtatcgtc acctgcgttg cctgctggga tcagacagac aaagtgtctt
    17401 acacctaccg caaaaaagat ggtgttgatc tggcttttct tctgtccatc accaaccgct
    17461 acggcggcat cgataaagcc caggcagctg cgaacggcta cgaagtgctt ccgggcgatg
    17521 cctggattga agaggaagtg gatatcctga tgcctgccgc catcgaaaac cagatcaatg
    17581 cgaacacggt gggccggatt aatccttccg tgaaagtgct gctggaatgc gccaacggtc
    17641 cgaccactct tgaagcggat gctgtgctga aagaaaaaaa tatctatgta attcccgatt
    17701 ttcttaccaa tgcgggcggc gtgacctgca gctatttcga gcaggttcag tgcaacatga
    17761 acttctactg gagcaaggaa gaagtgctgc agcggctgga aagcaaaatg gtatccgctt
    17821 ttcaggcagt tgccgatctg tcggagcggg aatctgtttc catgcgtgat gccgcctaca
    17881 tgattgccat tcagcgcgtg gcggaagcct gtcgcctgcg cggctgggtc tgatcggacg
    17941 gacatgtaca gagcaccgct ctgtgccaaa tagctacgag aaattcattt aactgagcgg
    18001 gtgaaggatc acttcatccg ctcagtcgat tttaagtgta ctgatatccg gaattttact
    18061 tttcctccgc tgttcccttt acaggcactg ccccccgtcg ggaaatgaag gaagaaactg
    18121 aaacaaaaga atccgctgtt gatgtataat taacgaagcg gttccgcatt tcttcagaat
    18181 cacacttcca actctaatta tctgcttgct ttttaagaaa gggaaaggtt atgtttcgtg
    18241 gaaataaacc aggctttaca ctcatagagc ttctggtggt cattgctatt atcggaattc
    18301 ttgccgccat cctgcttcca gcccttgccc gtgccagaga agcagcacgg cgctccagct
    18361 gcgcgaacaa cctcaagcag atgggcgttg tcttcaagat gtacgcaggc gagtcgagtg
    18421 atcttttccc ccgtatacat gccgaccagc cgtggggtgc ctccctgccg tcaggctgtg
    18481 aagacgggca gaaccgggcc agcctggcgc cgcagatgtc cgctgtctat ccggaatatc
    18541 tgacagatct gcaggtgctg ctgtgccctt ccgatccgga aaggagccag aagaacccgc
    18601 tgcatatcat tgctgaagcg ccggggcagc tctgcgccta caaaggattt ccctcccggc
    18661 ccgatgccag ttatctgtat tacggctttg tcattgacaa agcatccgac agcgacccgt
    18721 cttttgacgt ggggcttttc ggatttccct cgtcccttat ttcttcgcaa atggcctatc
    18781 tgatggcgtg tctttccttc atgccgctcc ttttcaacgg cacactgggt gataagaatt
    18841 cagagaatga ctgggcgctc aataaagacc tcgataaccc tgccgcctac ggtatgtttc
    18901 aggccatgtc caccccgtcc aatgttcctc tgggcaacgg agacagcacc acgctttacc
    18961 ggcttcggga aggcatagag cgttttctta ttacggatat caacaaccct gccgcctccg
    19021 cacaggcgca gagcatgctg ccggtcatgt gggatgtcgt ttctgcgacc accacggcga
    19081 gcgcccagtt caaccatctg ccgggcggcg ccaatacgct gtatatggac ggtcatgtga
    19141 aattcaatca atatccttat gagtttccgg cgtccaaagg gtttgcgtcg ctgggttctc
    19201 ttttcagcct tggcgaataa ctgccgacaa ccctttgtgg agaccggata catatgatgc
    19261 ctcatacgca cgtgccctta tccgtccgcc tgcggcacgg tttttcatac ttgctgcttt
    19321 tgagtgccgt ggttcttgcc ggtggctgcg ccagcctcag cggaaacact caggaccggg
    19381 ttcttgctgc ccgcaaccgg gtcgctcctg cgctggtgca cattcgtccg gtgaaggaag
    19441 tgtttgtatc cgggaagcga acggaagtgc ttacagtggg cagcggtttt atcatcagcg
    19501 cagacgggta tatagtcacg aacgaacatg tcgccggaga aagcagcgag gtatggtgca
    19561 tcctgagcga caatacggaa catgaggcgc gggtcgtagg cgtggatcct tacacggatg
    19621 tggcggtgct caaaatagaa gcagaccgcc cttttcccta tgtacgctgg ggggattccg
    19681 atgctatcga ggcgggacag atggtgcttg ccatgggcag tccccatggg cttgcccgaa
    19741 gcgtttctct ggggatcatc agtgtccgcg accgttatct tgaaaatgcg tccggcatgt
    19801 cttctcccta caacaactgg attcagaccg atgctgccat caacccgggc aacagcggcg
    19861 gccctctcgt caacttaaaa ggtgatgtgg taggcatcaa tgcgcgcatg ctcagcggag
    19921 ccgagaatct gggttttgcc attcccgcca atattgcgcg tcaggttgtg acacaaatca
    19981 ttgatcacgg cagtgtccgg cgcagtgcgc tgggcctggt ctttcaggag atgacttcca
    20041 aaaccgatga tccttcggca cggggtgtgg ttattgccga tgttgcgcct ttgtccgcca
    20101 gttatgaagc aggtgtccgg cccggagaca ttctcatccg tctgggcaat cgtcccatgc
    20161 atgcacgtca cgaagaggaa ctgcccgccg tccgaaaagc tgttgctgac ctgcctgtcg
    20221 gagaaccggt gactcttgtg ctggagcggg gcggtgaaga gatgtccttt tctgttgtta
    20281 cggaagaaga atcggcatac aagggcagaa acgccgagtt ttcggaatgg ggcttcacac
    20341 ttgtggagtt gacccctgat cttgcgcgcc gggcacagct gccgggtcat acgggcgtgc
    20401 ttgtttcggg ctgcctgccg ggcgggcttg ccgcatcgtc ggggcttacg ccgggggaca
    20461 ttattttaaa actggatgac acgccggttg cttccctttc agattttatg aacctctata
    20521 aatcggtgca ggaaaacagg aaagcgctga ccatgctttt tgtgcagaaa ggcgctctga
    20581 cccgttttgt gctgattgat cagacaaaca gcaagtcctc cattctggac aaggaagtac
    20641 ttgatcatgt cgattaaatc cttcttaccc tttgccctct gcctgagcgt gctgcttttt
    20701 tccggttcgg gcttttcctt tgaacaggaa atgctgcagc gcgcctatga caccatctct
    20761 cctgcgctct gcgtgctcac cttcacgcag gaaaccacag acccgcgaag cggcgagcag
    20821 atcagacaga acggttttgc cgtggcgctc aatgtctcgg cggaagggct gcttttaacc
    20881 aacgggcatc tgcagcggga caatgtgcag agttttaatt tccgggtcac tttccgcatt
    20941 gacggaaaag aacagagtta tccggctgtg ctgcttaaga agccggatga tctgaatgta
    21001 tcccttctgc gcctgcagtc cgaaacaccg cttacactgc cttatgtgcg cttcagcacg
    21061 gacgccgggc tttcgctggg cgagcccgtg gcgattctcg gcatcatggg ggaaacgctg
    21121 gatttcaacc gcgccattca ggaagaccgc atcatctccg ttctggacgc accccgatcc
    21181 acctattgtc tgggcggtca gctgaaaatg ggctttgtta ccggtccggt catcaaccgt
    21241 cggggacagg ttgtcggtat caccggattt gatcttgccg tagcggaggg cggtgagttt
    21301 ttttcgcgaa gcggtgtacc actggtatat caggcttctc tttttgaaaa acacatcgca
    21361 tcacctcccg atcagacaca ggaatccgtc gaagcggaag aagcctggct gggcgttttt
    21421 acgcagcctc tgaaagaaga atttgcacga tactggaata ttccggaagc gggcggtttg
    21481 atcgtgagca cagtcatacc tgattctccg gcagctgccg tcggcatcgt acccggcgac
    21541 attatccgtg cctttgacgg gcatcccatc cgggctatcc atgaccggga tgttctggct
    21601 tttactcaga tggtcaaaga gaaagcgccg gggtcggaag tggaaatcac cctgctgcgc
    21661 aatggcgaag agcttgctct gactgccacg ctggggcatc ggccccgtgc cgcacaggat
    21721 gccgatgaat acgaggatga gcatctgggc atggttgtcc gggagattac ccgcgacctt
    21781 cgcattctcc tgaatctggg tgaagaggtg cagggcgtga ttgtccgccg ggttgtgtcg
    21841 ggcagtgccg cacaggcggc acgtatccag ccggggctga tcatcatgtc catgaacagt
    21901 gcgacaattc gcgatcttga tgactataaa gaagccgcac aattttttaa acaggacaag
    21961 cctgatgaaa tcacactatt tgtccgcgcc ggagcacaga cgggttttct ccggctgcgc
    22021 ccgcgctggt aaaagaggca tgcctgccat gaacggaagc gtacggtttt ttgcggcgat
    22081 gctggcgctc ttttcctgta tgctggcggc atgccatcac aacccggatc cggatacagt
    22141 cctgctgaat gcgtggcgcc tttgcgaaaa gctttcctat acagaagcct acccgctggt
    22201 actggaaagc cttcaggcag cgccgggcaa tgccgtggcg cactatctgc tggggcgctg
    22261 ctttttcacc cagtctcccc cgcagcttac ccgtgccaaa ggcgaatacg atctggcact
    22321 tcagctgctt gacaaaaacg gcgatttaag tgtccttgcc ggaaaaatga gtgaagagga
    22381 attcagggcg acccttcatt gtgacaccgc tttagcactg ctgcagacgg ttgtggaagc
    22441 ggaaaaggcg ggcatgccgc cccgtaccgc tgtcggtgtc atgaaaacag ctctctttca
    22501 tgtgaatgaa ggactgcgct tcaatccggc atcgtctttt cttcaggaac tgagccagac
    22561 gcttcgccat atggtggatg tcatgaatga gtccgggact tttcaggagg cagagccgga
    22621 agcgccgcat tatttcatct gagccttatt gaatcacagg atctgccacc cacagacggt
    22681 atgaagagac cggtgttttt ttcaggccca gccgcgcacg ccagtcggcg ggatccttct
    22741 ctgtccagtc aacaagaatg gatgctgtat caggtgccag cagtgcactg tcgaaactga
    22801 tggcattcca tgcgccgtcc accggtgcaa gccggtagcg tgtggtgcgg gacggtgctg
    22861 cccggaacag cgcttttccc gtatctgcag attccgaagt gcttctgata cggacaaaga
    22921 gcggcggcac ttcccttttt aattcgggcg gcagcgcaaa aaagaggctg attgttctgc
    22981 ccggctgctt tttcatgggg aaaagcagcg cttttttctc cggaagcagg cgcgcggtca
    23041 tagtaacgtc gcctatcatg atctgttcag gtgttacctg ttttaacaga cttacgttct
    23101 ccagcgacag cagataactg cccgctgccc ctgccattac cacaacagac agccctgcag
    23161 gcaaaaacca gaacagcgat gcagcacggg ataccggtgt tccctgtacg gctgcgaaaa
    23221 aaaacatcag cttgaagaaa agatgcaagc ccacaaaaag tgcaaagtag aggagcggcg
    23281 taaccagtgc gggcagggaa gacgctgcgg caggaagagg cagccggaca tgtgccagag
    23341 agagaagcag gatcagcgcc gataattgcg acagagattc cgcaataagt acccccgcgc
    23401 cccgggctgg cttcaaaagg agcgtgcctc catgaaacag gcattgcaca agcgcatagg
    23461 cggctgcaat acaaagggcg acagcgactt ccgcttcaaa cccgtgcaga agtccggtgt
    23521 ccatgagcat gccgcagccc cagagtccca caagaaccga ggcaagaaag atgagcagta
    23581 tctgaactgc attagccata cagacaaacc cctccggttt tagatgatac ggcgcctgag
    23641 ggtgcgcatt gacccggaac agcaaactgt atcatttttt gtattctata aaacaacagc
    23701 ccgggcacac cggaaacaaa cagtttctgc atgcgcgccg gaaagcccgg gtctgtgcgg
    23761 aattccacat agtcgccagt ttttcttccc gcacattgcc gattttctga agccagcagg
    23821 ggaagacatc tcctgtccgc ccgataatga gtgtgttcca ggggctgcgg caggtatact
    23881 gccgcgcatc gattccgccg tcataatact tcagcagttc ctcccggggc atgcggggca
    23941 gacgcaattc aatgttgtga cgccgcgccg cttcacccgt ttccgccagg gctttttcca
    24001 gagcggtccg gtcaacacgg ggccattcca aatcggatgc gcgccacttt ccgggcggtt
    24061 cctccccgag accggggaga tcgtggagcc gtgtttcggt taccagattg agtgaatcgg
    24121 ctccgagcga cgccgccagt gcgggcatct ccgccagcac atgtacattt gcctgctgaa
    24181 tcaccgttgt aatatggatc agcgggcagg tcttgcccgc ttttttccgc gcttcagtca
    24241 gcagccttac gccatcggtg gatttttcga aggcaccttt ctgatgccgg atttcatcat
    24301 gcaattcgtc cggcgcctcc agagacacgc cgagggcatt caatcctttt ccgccgagcc
    24361 gggcaggcgc aagttccaca caggagtgaa ccgctgcctc tttcagcatg gtggcattgc
    24421 tgataaaatg cgtccgcctt ctgactgatg cataggcgag cagatcaaaa aaatccgaac
    24481 gcaccagagg ttcccctccg gtgaaggtga tgagccccag ccggttggtc tggtcgacta
    24541 catttttcca ctcgtccaga gacaattcgc cttttttctg ttcggcaacg ggcacatttt
    24601 ccagatagga cagatattgg cacatcctgc agcgcaggtt gcagcgccgg gtcacttcaa
    24661 taaaataatg ccaggcggga agcgctttcc cggacggaga caggcggtac ggtgcctcgc
    24721 tgtacatacg ctggaaaagg tcgtatgccc atgccatcat ctgctgtctg gctgtatctg
    24781 ccatggaagc tccttaatgt gcaccggtcg gtgaagattg ccgcccccgg aattatgatg
    24841 aatgtattgt ttaaaaaaca agccctgatc agtgagtatt tcccgggaga tcctcgtaat
    24901 acacctctgc cagcgccagc ccatggggag gcgcacagtg tccgagaaaa ggcgcacccc
    24961 gggaaaggct gtcttcaatg aaagacagag gaaacctgcc tcgccccact ccgatcagcg
    25021 tgccgcacag atttctgacc atcttgtaca ggaatccgtc gccaataaac tccagcgtcc
    25081 aggtgttttt cgcatctgca gggccgcacc aggcgccagg atacagacgc gccgagacga
    25141 gggttcgcac ggtggttctg ctttgcgaac cctgactctg aaatccggcg aaatcgtggc
    25201 ggcctgtaat gattgacaga ccctgccgga caaggtccag atcgaccggg tagggaacgt
    25261 gccagacaaa aagcgactgc agcggatcgg catggcggtt taatgagaag gaatagacgt
    25321 agcgtttccc tttcgcatca aaacgcgcat tgaaatcatc ccgcaccggc tcaagcgatt
    25381 cgatgcggat ttccggggca agcattttgc acaacccgtg tgccagccgc tgcggagggc
    25441 tgccctgcca caggcatgaa aaaacctgcc ccagcgcatg aacgcctgca tctgttctgc
    25501 cggcgccctg cactgcaatc ggtcggtcgg cgatccggga cagtgccgtt tcaatttcgc
    25561 cctgtaccgt cctgccgtta tcctgacgct gccatccgca aaagcgggtg ccctgatagc
    25621 ggaccgtagc ttttagacat gttgtatcca tagactggta ttgtactgaa gtgacacagg
    25681 ttcaggcgaa aagcctgcaa aaaaggacgg aagcgaaaga ggcatcatga cgaatgaata
    25741 ttttttctcc tgaaagcgtc tattttatca gaacaaacga tgcctgacgt gcgtagaaag
    25801 gagatctgcc atgaaactca aaaaaacaat cttctgtctt ctcctcatcc ttctggttcc
    25861 tgtgcctgca ggctttgcac agtatgtggc ggaaggtgcg ctcatcggcg gcgctttcgg
    25921 aacaggaacc ggtgctctta tcggaagcta ctcgggccgg acaggtgaag gtgcgcttat
    25981 cggagcgggc atcggcgcgt tggcgggtgc cgccattggc agtgccagcc gcccccacta
    26041 ctacagaagc acagcgccat acccgtatat tatgcccgtg cggcatcatg cctactatgc
    26101 accccgcccg tactatcaca taagaccgcc tctgcgcccg gtcgtatacc atagcgcccg
    26161 accggtgacc tggtatcgtc ccgccggagt ccccgtccgt actgcagtgg cagtgcctgt
    26221 gaggcgcgtc gccgtaccgg cagtacaggc gccgattccc cgtgccgtgc cgctggttga
    26281 cacccacccc atcgcgaccc ttcccgcacc aggtgcggca gcagctccgg cagcgcggtc
    26341 aaccgttccg gcacccgaca caacacagcg ttttacagtg cgcaccgtaa ctacgccatc
    26401 gggagaacgt tatgaagagc gtgtagccct tccctgagtc tgcagaaagg tacagcatga
    26461 aggcaagaac attatggcgt ctgacaggcc tcctttgtct ggcaggcgcc tttgtcctcg
    26521 tactcttcag tgatcccctg ctccggggag acgccgatta tcggttcatc atgattttct
    26581 ggggcagcct gctctgcctg ctgggggcag tggtctatat cgcttttctg gatattcatt
    26641 ttacacggct tgtctacaaa aaacaggaac gtgctctttt caggcagact tttttaggtg
    26701 agaggcacaa agaggaagcg catgaaaagg ctgatgaaag cgggtcctct gttcctcctg
    26761 aggaggcata aggcataagc cggacagatg cgacgttcag tcagcgcttt ccggcgtgcc
    26821 gaaacgccgt atcacatagt cttccagcag accgagaaac gaccgggtaa tttcctccat
    26881 gcttccctga agactgcact gcttttcacc gtccatatag acggggcata aaggcgcttc
    26941 gttgctgcca ggaagggaaa tcccgatgtg ggcggcacgg ctttctcccg ggccgttgac
    27001 gacacacccc atgaccgcca ccttcagtgt ttccacgccc ggatactgtt cccgccacag
    27061 gggcaggcgc atccggatat atgcctgtgt gtccgccgcc agacgctgaa aagcatcgcc
    27121 cgaggtgcgc ccgcatccgg ggcaggcagt gacggaaggc gaaaactggc gcaggccaag
    27181 tgcctgcagc agttcctggg cagcatagac ttcgccgcat cggtcgcctc ccggctcagg
    27241 cgtaagagaa acacggatgg tatcgcctat accggcttcc aggagaatcc ccatggcggc
    27301 ggcactccag atcagcccct ttgcgcccag cccggcttcg gtcaatccga gatgcagagg
    27361 ctgtcgtgtt ttttctgcaa gactccggta gaccgtgata agatcgacgg gctggctgct
    27421 tttacaggaa agaataatct ggttttccga aagccccgct tcaagtgcct gctcggcgga
    27481 ctgcagtgtt gacagcacca tgcaccggtt aaatacttcc tgcgcagcgc cggggtgctg
    27541 ctgtttcagg ttttcccgct gcagtttttc cagcaatgcc tgatccagcg atccggcatt
    27601 tacgcctatg cgaaccggct ttttcagatc ccgggcaatg gcgcaaattt cagaaaaatg
    27661 gatcgcccgg gcatcaccct gaccgacatt acccgggttg atccggtatt ttgccagtgc
    27721 ctcggcacac tgcggatgct tctgcagcag tttgtgcccg ttgtaatgga aatcgccgat
    27781 caggggcacg ggatatcccg catctctcag gcgcagcact atttccggaa ctgccccggc
    27841 ggctcggggc gtgttcactg taatacgcac caattccgca ccggccgcag ccagagccgc
    27901 acactgctca gcagtggaaa gcgcatcttc cgtgtttgta ttggtcattg cctgtacgac
    27961 aaccggcgca ccgcctccga taaggacgga atccaccaat acacgatgag ttaatccggg
    28021 catgggtcac acctgtactt ttttttaata agaaaccgat cacgcaattc tgtgcatttt
    28081 tccgcagcgg ggacatttca ctgcggcatg aggatactgg ggcggaattt tggctttcag
    28141 accgcaggag caggtgatca ggcggtaatg atgcatgcgg cgaagcattt cagccgtttc
    28201 ccgatggcgc tgccgggcat cctgatccgc cggaacggaa gaagccgcag ctgccgcact
    28261 gtcctgagct gccggaacgg cagattgtgc tgccgtaaag gccatggcgg aagcgggcat
    28321 atcaacggct ttgcttccgc tgactttttc ccaggcagcc gcataatccc tgtatgctgt
    28381 tccgccgctc atgttccgca agatgcggat acgctcatcc accggcgggt gcgtgctgaa
    28441 gaggctgtta aaagaacggg cgctctgcag cggattgatg atatacatag gcgccatggc
    28501 acggctcgtt gtctctttat caccgggatt tccggcgatc acttccagtg cgcttgccag
    28561 cccttccgga tagcgtgtga atacggcgcc gcccgcatct gccagatatt cacggcgccg
    28621 ggagcaggcg aaatacagaa tctgtgcgag caggggagcc agaacggcga gcaccatcga
    28681 aataaccagc aggacaagaa gggctgcatt tccgtctttt cctgaacggt tggaactgta
    28741 gcggcgcatg cttcccgtgg tccgggtcgc ataccagatg gaacgatgaa acagttctgt
    28801 gatcagaata atggagccca gcataatgcc tgcaaaggtc atgaactcgg tgtcccgatt
    28861 ggcgatatgg gctatctcat gggcaatcac accctgcagc tgatcccgat tcagtttgga
    28921 cagcagcccc gccgtcacag caacagctgc cttctccggt tttctgcctg ttgcaaaggc
    28981 attcagcgac atgtcttcga taatatagac ctgcggtaca gcaggaagcc gggcggcaag
    29041 agtcatctct tctactacat taaaaagctg cgggtgatct gccttttgaa tctgtcgggc
    29101 acctgcgact gccatgagga gccggtcgcc cgaagaccag gcgatcagtg cctgcagtgc
    29161 ccaaatcata aaggcaacgc ccatgccgat gataccaccg gcaagcgcct gttcgggagt
    29221 gggcagcata agcgcgccga tggtaaaccc cacgcccagc agcacaagaa acatgcctgc
    29281 gaggagccat ctggaacggc gctgattcgc ctgaatcaat tcaaacatga accgcccctt
    29341 tgccgattgt cgcccgttac gccggggaca aagaaactac gctcaggaaa acgatacttc
    29401 aggaacagca cgttcggctt caacctcaat ttcgaagaaa ctctccttgc tgaaattgaa
    29461 catgcctgcc acaatattgc ccggaaacat ttccacttta ttgttcatca tcatgacggc
    29521 atcgttgtac gcctggcggg caaaactgat gcggttttct gtggaggcga gctcttcctg
    29581 cagattgctg aacaaggtgt ccgctttcag atccggatag cgctcgacca ccaccataaa
    29641 attgcgaagg gcgccgctca atccgctttc aagggcgcct tgagccgatg cacccgccga
    29701 atttttcgat gcttccgccg cctggttccg tgcttttatt acgctttcca gcgtttcccg
    29761 ttcatgcttc atgtacccct tcgccgtttc aaccagattg ggaatcaggt cgtgccgccg
    29821 cttcagctgt acatctatct gcgcccaggc atttttaaca gcattgcgaa gtcgcacaag
    29881 tccgttaaaa atggcgatca tccaccatac aaaaataagc agcactgccc ctgcggcaat
    29941 caggataatc gtacctgtca tgatgaaact cctttatccg gttgattttc aacgccatac
    30001 tgctccgtgg aaggcggaaa catctcctgc agccacagag aaacagtgcg cacgtcccct
    30061 tctgtgcagt ccgactgaat aacagcaagc aattctccgt gatcaaacca aagcccatgc
    30121 ccttttggac agcggtcata aacaattggt ttcgcgcctc ccgtcacttc tttgtccatg
    30181 gcatcatcgc acagagggca cagccgtttc ttttcaccgg ctgccactgc tttgctgcct
    30241 gctgtcagaa acgctttcga cagccgtgcc tcgccgagaa gcagctcaag ttcccccgca
    30301 tcgagccaga caccctgaca ggcggcgcaa taatccacct cgacatcctg atattcgagg
    30361 gtcagcaata tttcgaaaca ggctggacat ttcataaagc tattgtatct ttaaaaatcc
    30421 ggaataatca aagaacagca gtatccccaa aaataagcat cacgggcgaa ggcgaacgaa
    30481 ggcgaacaaa agaaacacct cgtcagtaag ccttatcgat aaggtgtggg cctaaatggt
    30541 atgcacaaag aaaatcctaa aatgtcaaca atgtgaagag atgatatgac aaaaatcgat
    30601 tcgtgtgaaa tcggcgaagg atggggctcc agtggcgggg tttatgcggg acactctttc
    30661 cagatgcgcc tcgttgaagg gcctgctttt ctcagcgcct tgggcgaacc atgcatcggt
    30721 cggctagttt ctttctcaat gccatttact tgtgcgttgt gacacgcgct ttcaatatcg
    30781 ttgtttcggt gaagaggact gttctccctg atttctgggg cgggcacgca ctcaaaacga
    30841 tgctctctta cctgcaaagg acaatcttcc tgatgcggac tgagataatt caaaaacgta
    30901 cccaagcctc ccgcccaaac gatctacagc ctccttatcc ggctattagg gacgatttaa
    30961 aaagttgaca agataacgta ccatagtttc tgtaaattat ttggacaaac ggggtcatcg
    31021 tttg
//
LOCUS       DAOPWB010000016        30462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1430_length_30462_cov_5.816523, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000016 DAOPWB010000000
VERSION     DAOPWB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 30462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30462
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_30462_cov_5.816523"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PKN92_02900"
     CDS             complement(<1..618)
                     /locus_tag="PKN92_02900"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47772.1"
                     /translation="MNSKKTATVTLLCVMLLAYIAMPLAHGQDVEDAQPAVNGAPVME
                     TTKLDATVTEAEGYFRRGVDLYKQDLFREALTEFNRALALDPNFAEAREFQSKANIKL
                     QQALVGAATAETPVFQTIEPTTLRANEGRMDLSADEIRQERVRELMSHGAKYLEAEHY
                     TTAVEIYSNVLLIDPENQAAKDGLHKATLGAHDTKIKDNKSGVAED"
     gene            complement(693..1217)
                     /locus_tag="PKN92_02905"
     CDS             complement(693..1217)
                     /locus_tag="PKN92_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47773.1"
                     /translation="MDKLQKLGAWLWRNKERMVLIVMLAVLAYRVYGIFNPPPEEAWA
                     RLPQPKLELPTEHDERQELGLPNLPALPAPSGLPGVYTSLYERNPFWYYSGQKTSSAS
                     EQVSAADLNIELVDIQNVRGKPRARLKTSRTTKWYSENEQFEEFEVTSIDAENQSVVV
                     YSQRYSKSFTLEKQ"
     gene            complement(1280..2266)
                     /locus_tag="PKN92_02910"
     CDS             complement(1280..2266)
                     /locus_tag="PKN92_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47774.1"
                     /translation="MKYKAQIAGLLILALIGGGFFGVWHFFFKELFAGYQQDSALKDS
                     LEATLKQMEDNFSGYKPELLIDEWQNMVQPWRNAREERAKFFNFGGWYDIEIKPQEDR
                     ILKFWYTEETNTMLMELYKKVYEKMGGYDRFPLDLRAMLNVPKEEDWSGQNVTKEQID
                     LSLRKLQFGIQISEFLLKANIGSLTTIAVWPRRFPEAHQQMLGLQTVGLNFTMTARDL
                     VRFLESLRQEQRYFTVDALRVTYPYISYAGEPQLNVRLLLTQANYRPPHEESAPGAAV
                     AGAPGAAFGPGGSPMVIPPREIDPDRITVKPEELGPVGRFWKWFKRTVLYMN"
     gene            complement(2263..4293)
                     /gene="pilM"
                     /locus_tag="PKN92_02915"
     CDS             complement(2263..4293)
                     /gene="pilM"
                     /locus_tag="PKN92_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNZ47775.1"
                     /translation="MARMWSGLKKSAGKKRLVIDVGTSAVRVCELAKTKTGYEISKYV
                     QREYNSDPSLEEQARNQLRSNALLEALKESKIRRRKTVLGVPGQSVFTRCRTLPPVAE
                     FKVNQIVRYEIQQQIPFGLDQIAMDYQVLQKTEQGGYDVMMAAIKVDVVEKHLEILKA
                     AKASVDTVDVLPIAAYNWLLHQKEMGQQGDCVALIDIGASTTDIVIERGNQFRFTRPL
                     NIGGNDITRALAEAFNLDFISAEKIKRERAFAPTGDPQRDGKSGEVIGQVLQRLSTEI
                     IRSFSYFRSLPGGGQVNRILLTGGCARLKNIVPYLRSQLGMDVRIAPVINNISIGEGA
                     SSVKAAPEQACVVLGMAARCWEPVAININLIPPRILETARRKDQALYWGLSVVALILI
                     FASMVPSAAKENKRVKQRIDELRSAIRAYDPELVQRIRVGVPVPQSELRSQLDVRKRQ
                     LQTLVGQVDVLEKAKRQRRFWLDEFAFINEARPTLGQMWFSSMESCVVEEEKPQQQHG
                     PAMRPPSDEEGSSVAPQRRGFPGLQFPGASGAASDQQGMGRGRPGGQAEEKVNDYAIP
                     RANGVIIHGFAESDEVINEFVKGLRTTSRQMPNTWYVSASRVIFSEGSVRRVPWSILN
                     DAPTEGGITDRGAPGGAGAQGKESLFTFTIEVALHYTADKPSPPTPTEDGTV"
     gene            4829..5935
                     /locus_tag="PKN92_02920"
     CDS             4829..5935
                     /locus_tag="PKN92_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47776.1"
                     /translation="MILTVSLFVLNATLCQSGTIAFITGESPFDYQLACLDLGSGEVR
                     TPGRGLSDSAPQWSPDGSRVAYQSRIEGGYSVCVYSVDTETTEILPHTYAWNYEPAWS
                     PDSRKIAYCSEVGDTPLRSLAVYDLESQREELWGGGHEGLLSPAWLPSTDMLKALDPD
                     DQLAADSLGLFALKEEAEKNGALLAIGVAGMPPKLSTEIFIVTRDQVIPLLSLLTGQS
                     TRYVRYAVTPDFKGRQIAYECNEGGDREIFMIGRRGIANLSNHPAADWNPKWSPDNNW
                     ITFESFRNGYSGIYLIQATTAGVTAIAGAKEYQCWSPSWSPDGKWIVYVSNESGMPQL
                     YASSFDGITQKQLTHEKYGALNPVWRPAPQKSKD"
     gene            5941..7005
                     /locus_tag="PKN92_02925"
     CDS             5941..7005
                     /locus_tag="PKN92_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ47777.1"
                     /translation="MCNCRFVFLAGCLAFCALTAQARNTEGALDLIRVPNNGQPVQVL
                     RESTFEVILREACELSLSGETGVYPMAVTWHDKRGDRVSGSAVVPSHVPSGTYALQGE
                     CQNIRDVNLRSVYVVDAFPDAYALAHITDMHTGSGRHKRSDRDILIELIQQVNAAEAQ
                     FALITGDVTDGGTPEEFRRFLDILDTCRMPTYITPGNHDRTGTHYADYFERLTYAFQY
                     GRDGYLSFDTKDYLIADEMGVQNAHLYLYRRQLRPCRWSIGLTHRYDPGMGIHAQLTL
                     FVDDPLDYLLVGHTHREARMDDQIPWGKTDLIMTPAAINGSWRLIRVDSQGLHPEATV
                     STVSVFQEKDELQDEEREDQ"
     gene            complement(6999..8249)
                     /locus_tag="PKN92_02930"
     CDS             complement(6999..8249)
                     /locus_tag="PKN92_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNZ47778.1"
                     /translation="MRRGFLQFVGVVLFLCLAGAPLHAQEATVMNNEGVDAFNRGDFS
                     RAVMLLEEALALDYSNTAIRRNLCNAYQKKAELLAAEREFTLATQILRKAVKVDAENA
                     SPFMQMGAYYLSQDKVADAIDALEEAIGLAPGNLMAHELLGQAYYEDNDLYSARVQWD
                     YVLAVDPERPGLASRYEKAFREESVEQEFNRWKANHFIINYPKDTPARLRGAITTILE
                     RAYLDVGRALGGTYPPSPVQVVLYSFEQFGEATRMQGHIGAVYDGKIRSPLTDTDGEW
                     IAEDELKRRLVHEYVHVVARQIAGDRLPWWLNEGLAETLSKSLVSRDIDQLKAIYNED
                     KALPLASMESAQMEHRSADDLRRAYLQAHATVDLLWNRYGRNKMLVFLRSLARGEESE
                     SAFKSIYRRNYETCQNEVAALYLY"
     gene            complement(8249..8638)
                     /locus_tag="PKN92_02935"
     CDS             complement(8249..8638)
                     /locus_tag="PKN92_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005503164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNZ47779.1"
                     /translation="MKQCIVSPNGPPAAGPYSAAVRAGNLLFISGQGSLARDGSGYVA
                     GTIEEESRNALENLKVILQDAGLGLENVVKTTVFLRDMADFSRFNTLYKEYFTRDCPA
                     RTCIAAAGLPLGMQVEIEAIVAFPEEK"
     gene            complement(8750..9718)
                     /locus_tag="PKN92_02940"
     CDS             complement(8750..9718)
                     /locus_tag="PKN92_02940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ47780.1"
                     /translation="MANVEISVIVVAKDITDALPTLLTCFEQQSMPTARYELILVSYG
                     EAQPDAMARIAKCLTGASISVKHYHEPSSSEVKAKNVGARKASGALLLFLDPDLLAGP
                     ELLERHVEIHATTGKPVIAFGQSAPHSALPGGLLTRWFVKQDHALMNVEKPESLYTLS
                     SRHCSLSRGLFLEEKGFPEDFEAMRAADILLMRKLIEKRCVPLRLPGVQAFIWRASNF
                     DEECARHYREGYDLARLAFLLDDPELLKNFGLQCSSFRYWLAELYMPFYVRTCRSHTL
                     DMRVHGHSCRRVLVYERHRGARAAHHRYGSGRNEVLRWSGKTTVEE"
     gene            complement(9853..10074)
                     /locus_tag="PKN92_02945"
     CDS             complement(9853..10074)
                     /locus_tag="PKN92_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47781.1"
                     /translation="MAAVLITPLVALLFMAIIFGLWTGTQLLAEKRLGYRVQGCKGPT
                     RGARGESLCCKGGGRLCSEADDGHRHLTH"
     gene            complement(10148..11170)
                     /locus_tag="PKN92_02950"
     CDS             complement(10148..11170)
                     /locus_tag="PKN92_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ47782.1"
                     /translation="MKVIIQIPCYNEEKTLGITLDALPRTLEGVDSVEWLVIDDGSTD
                     GTVDLAIRKGVHCVVRNRRNRGLAHTFLHGLNACLQRGADIIVNTDADNQYCADDIQK
                     LIHPILTGEADMVIGARPIDDIRHWSFQKKILQKIGSKVVRLASGTEIADAPSGFRAI
                     SREAAVRLNVFDNYTYTLETIIQAGRKGIPVMSVPIRTNDELRPSRLMKSIPEYVRKS
                     LFTVVRIFMTYRPFRFFAMPGLLVFGAGSLLLLRFLLLYWLGDGAGHIQSVVLSALLL
                     GTGLALMVVGLIADLIGVNRQLLEDIRSRVRELEYTLTRLEKSTRPQSEADNTEGEKC
                     PDETIR"
     gene            complement(11296..13860)
                     /locus_tag="PKN92_02955"
     CDS             complement(11296..13860)
                     /locus_tag="PKN92_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ47783.1"
                     /translation="MISQYGRRATRRKSWFRSSLFSQLVTFTLGAIVGACLYYAYSEI
                     YSEKVQEQQPTAQVDTSPEPVIPAQAVVPEVVPPVQPEYAIEPIPADMDSMWPLRHVI
                     VGVEGTHLSRKTIEMLNAWKPAGVWLRENNTENPRQIEEMISLINLYASLDGSREKNP
                     LICMAQEGGQTGNLLQIDGALTCQELAALDSLDAIKKAGRQVAETALSAGVGVLLAPR
                     LDLFFPGRSREEDRPHYFGDNKESVIQAGLAYAGGLMEAGALSVVSSYPGMGSAVHQP
                     RRLPVVEETDIEKLAEAMLPFAEAAAYDISGILVGNVSVPALDKERPDRPASLSPRLV
                     REVLRRQFGYTGVVLASDIIEAGVWSGNEPETDLVDALKAGCDAVLISSANTEQLGRI
                     AQEVMRAADRGILNKSELDASARRLDSWRQQIARQRSAVPAEVEGEPHDVTETIPESA
                     PAVPEADVVPETVKTAPAEKETAESAEETPAPSSETAPPRSTIIDQEAVPELVEEKQP
                     QAKSPEEVEATPAAVQEAVPVIEETAPPQEEKPAAPQEKAVVQETKKDTPSPQPQGTK
                     MLSYTIQPGDTLSKIATTHQVSSSDLTKWNGLTNGDIKFGKKLTIYVPEAGNAPEQSQ
                     PSGAEVSVEKTVEDVVTEIISPDHAAAAETESEGIAEAAPVQETAGNSPETAAALEAV
                     TTEQVETEPVTAEDTSSVPEAEEIPPPLTPSRETEKNSADEVLPEEATDVISDLDSQT
                     DFQPASDLLEPSDEKQDERPESMIPPVSPPVEYTQAPDDAPVQAENSADENKEASSQT
                     EDTATPEFGSYVIQVGDTLHSIAKRYNTTVQNLMAINGIQDANLIVLGKEIKVPLP"
     gene            complement(13926..15125)
                     /locus_tag="PKN92_02960"
     CDS             complement(13926..15125)
                     /locus_tag="PKN92_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ47784.1"
                     /translation="MNVAKSENNSLPGRPFSGLFQFLLICLPLLLFFGGLEIIARFYE
                     SAHPVLQVDIGQGFDESSLLFAPVPGGWMETRPEKTVSFKQQRFKMPKPPGVLRIFAL
                     GGSSVNYLDYEFSELEKELEHRFHASVDAVEVINCGGLSYGSHRLVLIAAEILHYQPD
                     IVLLYSGHNEFEELQQLHLSLKNYGKVQQYLGKSAFYRLIREMRARKAIARLEESRQA
                     RDLAQSIPDSSKTWEMVFTQEEIDGRMEAYRQNLTMIIAQCREAEVPLIIGTVPSNLI
                     RPNLPGKEGQLYEEVVQLYEAGDFDEAKRRGAEILAQASPRHQSSPVENGIIRDLARI
                     YGVPLADVETAVASAEPHGIPGETLFSDHCHLNERGNRILRQQYQALIEDIVSRRYAP
                     EPSLGIQ"
     gene            complement(15236..16519)
                     /gene="purD"
                     /locus_tag="PKN92_02965"
     CDS             complement(15236..16519)
                     /gene="purD"
                     /locus_tag="PKN92_02965"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNZ47785.1"
                     /translation="MNVLVIGSGGREHALAWKIAQSPLVEKVYGAPGNPGIDRLEKGA
                     CINVGVNDFEALDTYIEAEGIDLTVVGPEVPLVHGIVDFLEERGKRVFGPSKKAAQLE
                     GSKSFAKEFMARHGIPTAESRTFDDLQEALAYVRSQPAPIVIKADGLAAGKGVTVAVS
                     QAEAEAAIYAAMAEGAFGEAGARVVVEEYLTGEEASILAFCDGRTVKPLASSQDHKPV
                     GENDEGPNTGGMGAYSPAPVVTDALMEEIVWDVLVPTSAGMAKEGMPYKGILYAGLMI
                     TGNGPKVIEYNVRFGDPETQAILPRMETDLVPALLACCNGTLDSCEIRYSPRPCVSVV
                     MASEGYPGVYAKAKEITGVDEAEALEDVAVFAAGVKRKGDQLFSSGGRVLNVTALGNT
                     QRAAMEKAYQAVSCIHFEGAFYRRDIGAKALKHLE"
     gene            complement(16754..17719)
                     /locus_tag="PKN92_02970"
     CDS             complement(16754..17719)
                     /locus_tag="PKN92_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNZ47786.1"
                     /translation="MNHQKKSLMAVEIGGTKLQAVLGLSDGTILERRRGQVNPEAGAE
                     GILRWMREQCGELLALASAKEAVQAIGVGFGGPVESSTGRVLVSHQIEGWQNAPLKHC
                     FEEWFHLPVAVANDANAAGWAEYCCGAGRGTRTFVYMNIGSGIGGALIVDGKLYDGQG
                     LGACEIGHTWVPDWTSAQPGAADKLENLCSGWAIERRIRGTMLPEPGTPLHALCDGDA
                     ALLSCPMLAQAAQAGDQAALDEIGHVAEAAAMALGNVVTLLHPERIALGGGVALMGDV
                     LLKPLRSALSARIFAPFNDTVQFVACELEEDVVVVGALLLAGDLL"
     gene            complement(17716..18825)
                     /locus_tag="PKN92_02975"
     CDS             complement(17716..18825)
                     /locus_tag="PKN92_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47787.1"
                     /translation="MPRNTAKKLCAAEKLHWLPDGIYPRWQDEVTGVEGVVLVRGVPE
                     SEVVYQTHPMWTPEMDYFLFMARPEQGGLHPHLLEIRTGESRLLLDGNVHFAAMAWRS
                     RSLYYFADGYFHEADVVDSFQGRGEPRVLGALPPECARLAGALTVDASGEALYFGASL
                     VSEGEWGIYVLSLKNGQSRLLCKTDFRIGHFQANPYRPGSLMFCQETGGDANQRIWHI
                     QDGGSAMKPLYQEEGWEWVTHECWWGQDRFLFTVWPYDEERKNKPHGIMMADLESGPQ
                     GKMTEIVRYPAWHTHGSPDGKWLLGDDFDRSLWLINGEEKERRLLTAGHTSGDFKTHP
                     HASFTPDSRAIVFSSSKFGAREILLISLPDWKDLK"
     gene            complement(18966..20336)
                     /gene="hemG"
                     /locus_tag="PKN92_02980"
     CDS             complement(18966..20336)
                     /gene="hemG"
                     /locus_tag="PKN92_02980"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HNZ47788.1"
                     /translation="MDTEVVVIGAGVTGLCTATLLAQKLGREAVLCLEKAPHPGGTTG
                     SDRDGRFVMDWGGNGFLDREPRTLEWIEQLGLTNQLVRANTAAAKRFICRNGVLHEIK
                     APPGFFFSPLLSVMGRLRLCLEPFIPPKKDNSPETIYGFASRRIGREAARIMVGAMVL
                     GIYGGDAEKLSMTHCFPRMVAMERDYGGLMKALLAIRRKRKASPMGPPGTLTSFQGGT
                     GTLIEGASAQLQDRIRFNTPVSALARGDNGYEVILPDGSVIRCRAVVSAVPAYAAAAF
                     TSDFDAALSGALGAIPYAGLAVVTLAFPKAAIGRDLDGFGFLVPRGEGIRTLGCLWTS
                     TLFPRQAPENMALLRVMAGGSTDPEVLDLSDEELADLVLKELTPVLDIGGKAEFIRIK
                     RHEKGIPQYTIGHEQRLEAIEAAESRHPGLSFAGNAYRGIGLNDCVLSAERAFLRTLE
                     CLHRSD"
     gene            20628..23174
                     /locus_tag="PKN92_02985"
     CDS             20628..23174
                     /locus_tag="PKN92_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ47789.1"
                     /translation="MFATTQDLMFSPAFYETGTGCIWTRPHSELLSMNEWFLAGPAPG
                     IYRDEWLEAAADYRDQVSQGGKRFLLLDTARGVARVQPSSALTRLLKLQPGDELFYSF
                     RARSGKESVFSLTLEEITHEQGSPDNWQKTLQTLHLPGDGEWHELSWSVNIPEDVPET
                     CIAAPVLTLMPGSDMSASAEIAGILFGIGDTERMSLLGNHLRFMNSTHMCRCIYNRDD
                     LEWAGSAFSGYFLFAYDRRFYTPEEGYKTQALIEDFQERLGGVDTVVFWPSYPQLGVD
                     DRSQFDFFDDMPGGLTGLREQFRLMQEKGIRILLPFLPWDTGTRRENDEAEKQLIALV
                     AALEADGIFMDSLSGAEDRLRMATDSEKLGVVFVPGACPPVEQVSVCNLSWAQNAEYP
                     IPPGIPLLKWVEPRHMQHFVRPFSLDRREEIEQAFFNGSGLMIWENVFGFANPLSEED
                     AALWKRCVSILRNMHKPFTSSLWDPFFPSLQEDLYIHRWPGAPGTVFTLINKGEPLEY
                     VPLIRWKLPESLRADQMVVYDLWNGGTLRWDMNEFGAVQVWGDVERLGCIAISFSPDE
                     NLENLAAQRKKEQLPEASSRHQYLALPQRLAAPAANASSNTGGTDGMRFISGGGITLA
                     AANPGNPADERLLPLQKQAEAETHQVNPFYMDEAQVSNGAFQVFLQETGYHPENDQNF
                     LKHWGGRSMPDALADLPVVYVSLEDARAYAAWAGKRLPTEEEWQAAAQGEDGRCWPWG
                     NDFSSQHCAPAGTAPVSVRSCASGRSPYGMYHMTGNVREWTESEYSDGITRFCILRGG
                     SCYEAKGSRRYVPGGPQPVTARTKYLLMHPGLDRSSLIGFRCAKDAVSAG"
     gene            23265..24242
                     /locus_tag="PKN92_02990"
     CDS             23265..24242
                     /locus_tag="PKN92_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ47790.1"
                     /translation="MNVQKAVITAAGRGVRLFPVADTVQKGMLPLQDRDGLVKPVIQI
                     IIEEALESGIEELCIVCAPGDESRYRQQMQQIRDNLLEGYSGEQWAQQQAARLEVILN
                     KLSFAVQEEALGYGHAVYCAKDFAGDAPFLLLLGDHLYVSHLEGKRCAQQLLALAAQE
                     QCAVAAVNPTREHLVGNYGTVSGKRVPAMPGVYTLEKIIEKPSVSLAESVLQTPGLRA
                     GHYLCFFGMHVLTPGIFPLIEEEMAQSKRSNSSCQLTPALQALALREKYLATEVQGSR
                     YDIGARYGLLLAQMALGLAGQDRDVMLSDVVSLLAECRCNSSPSMERQV"
     gene            24239..27574
                     /locus_tag="PKN92_02995"
     CDS             24239..27574
                     /locus_tag="PKN92_02995"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNZ47791.1"
                     /translation="MNAFIETITSRDEVLRNRPFRTLCAGRNSAELLAAARELEAFRQ
                     SSENLYERVRASLFLYALHRFYLSESADFPASGTLPPSGFYDLMERRFEEALACFWKE
                     VNEQGATGALCSALAESYHHLSFDTLSDQVRRSVRSSRGNQWMFRVGHPAEHPLRCRP
                     ELQRRASGESLYPVLVERTPVRLDLSHSGWSDIFFLGMDYPEGARVLNISVDLGVYGR
                     DSSTRPPVQAYARVITEPVLRITSLDLGTSKDIRDLGDLFNFGNDYLSLLKAGIIASG
                     FIPPSFEGTDIPLAGILGEVIRPGMGMELVTQVNDIPKGSRLAVSTNLLACVIALLMR
                     ATGQTQSLTGSLTEEERRLAASRAILGEWLGASGGGWQDSGGIWPGIKVIRGALAGES
                     DPEHGISKGRLLPEHQILGDQDLHPDITERLAASLVLIHGGMAQNVGPILEMVTEKYL
                     LRSQPEWDARLRMDKIFNGIRSALKTGDIKALGACTTQNWNDPLKTIIPWVSNHFTES
                     IISRARAALGDDFWGFLMLGGMSGGGMCMMVAPERREAFRDIILDIMQSTKEELEEAL
                     PFAMNPVVYDFTINNQGTQAALLTGEEALLPMSYHILLTPDLARNCPENLPLQRRIEL
                     DCAIAACPEPDKRDQLLRTLVGSLFRVGDALSRSEKNQWDEEAEAIKAQNGFDSIQHE
                     MIRDDLRGGRIGLSRNRLPADTLIEDVQDQDILFLKADARSRQSGEEALRAGTVAVLS
                     LAAGVGSRWTTGAGVIKAINPFIFMAGRHRSFLELHLAKTRESMQRYSCVIPHIVSTS
                     FLTHGPVVKHLEQQKRYQHPGPLYLSPGRSIGQRFVPMVRDLLFLWEETTQESLDEQK
                     QKVRDAVRAALIDWAKAKGEGTDYTDNLPIQRFNPPGHWYEVPNLLLNGVLAQLLQDQ
                     PQIKTLLLHNIDTLGADLDATALGMHLLSGNALSFEVTPRRIADRGGGLARVNGRLRL
                     LEGLAQPREEDELRLRFYNTMTTWIDVDALLQQFGLVREDLCRSRDKVAQAVRMMARR
                     MPSYVTIKDVKRRWGRGQEDVYPVAQVEKLWSDMSALPDLSCGYMVVPRFRGQQLKDL
                     NQLDAWANDGSKDWIARLCGLQG"
     gene            27673..30135
                     /locus_tag="PKN92_03000"
     CDS             27673..30135
                     /locus_tag="PKN92_03000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ47792.1"
                     /translation="MNPSFKEKYARVPASPLLWFLLLCAFHFSFNIWWLHADRHAIST
                     DEETHMIMARDYYTALFLPPGNESISARFEALRHIRADVGNPVHPPLLHLAGAFLAKI
                     LGYGIDRIAFANTLAFLLSLAGFYLLMGSFLSQKNTLFAVTVFSMTPMVYMASRYFMT
                     DFLSMALVIWVLYTLWKSDGYARIGWTVLFGICNGLALLTRTTAVLYYALPCFLVGCF
                     GVYRIFQESSGFASRWKELMKMSARMALAAGITLLLAAPWYIMHGEQFYQHWMKPQAV
                     HEETPLSILRKTAPPPQQTAAASKTEATASAADGQKENSSPKGETGKAGWRLRLHRRI
                     PWTRYPVFVINNAVFLPMFLLSLAGMICACCSGAFIKRKEIWLLLSWLWGSYVLLTLL
                     LSFSTPRYALQALPALAFFSALPFFLIQGGKLRRTLQGVYLALLLFQFVTLSVHAFSP
                     LPELKLPLQPDPEFQAVYDDPGLYIYKSVLHASSAYGRMQAPMQDNFKDRIFEAMLQN
                     EQKQPYFGVEAPYARLNMRGMLFEEQHYWLDSSRPNPFRRTDIPADLEPYRNFRHYGW
                     GRDMEEIQPILPMVDYVVYTTEDISEAKEQEWLHILDSHDFDLIERFFEARFGQVGAR
                     YYAVAARREIRPLPEIKTQADIAALPLEALYRFRHSGKWRLLSPPLQNMLEQRIQALF
                     EARGKGVTLNDTVTFLGATASRASEDRYILNLMFKSNDPPPLSCRLLLQGVVSPEDMA
                     VHFGTPGEKYGQFRWSFEPSPSLVFWPEQGHVLVRFPFSARPIPYELSLAFFLPGKGL
                     WDRAVNLGSVDFSKVPDENATR"
     gene            30154..>30462
                     /locus_tag="PKN92_03005"
     CDS             30154..>30462
                     /locus_tag="PKN92_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNZ47793.1"
                     /translation="MTVSARRQPASDNDPDFRRLVRGAFLSAISILISMAALLITGKL
                     FTNALDQEQVGIFALLLVCSDFIIYAAGLGFAASMPKLIAAAGQEDRKKIIASALAGQ
                     L"
BASE COUNT         6957 a         8545 c         7727 g         7233 t
ORIGIN      
        1 gtcttctgcg accccgctct tgttgtcttt tattttggta tcatgtgcac ccaaagtggc
       61 tttatgcaac ccgtctttcg ctgcctgatt ttccgggtcg atcagcagaa cattgctgta
      121 aatttccaca gctgtggtgt aatgctccgc ttcgagatac ttggcgccgt ggctcattaa
      181 ttcgcgaacc cgctcctgcc tgatttcgtc ggcgctcaaa tccatgcgcc cctcgttggc
      241 acgaagcgtc gtgggttcaa tggtttgaaa aaccggagtt tccgcagtag cagcaccgac
      301 aagcgcctgc tgaagtttta tattggcttt gctctggaat tctcttgcct cggcaaaatt
      361 cggatccaaa gccagagcgc ggttaaactc cgtcagcgct tctctgaaca agtcctgctt
      421 gtaaagatca acgccgcgcc tgaaatagcc ttctgcctcg gtgacagtgg cgtcaagctt
      481 cgtagtttcc atgactggtg caccattgac cgcaggctgc gcatcttcga catcttgtcc
      541 atgagccagc ggcatggcaa tatatgcaag gagcataacg caaagcagtg tcactgtcgc
      601 tgttttttta ctattcatga cggtcttttc ctcccgcggt ttgcggcatc ttctctttac
      661 ggtcatgcct tcatgctttc ttcgggttaa cttcattgtt tttccagggt aaaagatttg
      721 gagtagcgtt gcgaataaac aacaacactt tgattctccg catcaatact tgttacttca
      781 aactcttcaa actgctcgtt ttcagagtac cacttcgttg tccgtgaggt cttaagtctt
      841 gcccggggct ttccgcgcac attctgaatg tccaccagtt caatattcaa atcggcggca
      901 cttacctgct ccgaagccga acttgttttc tggccggaat agtaccagaa aggattgcgc
      961 tcataaagcg acgtatacac gccaggcaat cccgagggtg ccggtaaagc cggcagattc
     1021 ggcaaaccaa gttcctgcct ttcatcgtgt tctgtcggca gttccaactt cggctggggc
     1081 aaacgtgccc aggcctcctc cggaggcgga ttaaaaattc cataaacacg gtaagcaagc
     1141 acagcaagca taacaatgag caccatgcgc tctttgttac gccacagcca ggcaccgagt
     1201 ttttgaagtt tatccattta cttcagtttc ctgaatcagg ttagtgtata ccaatacatg
     1261 acaattcaca acttttttat caattcatat aaagaactgt ccgtttaaac cacttccaga
     1321 agcgaccgac aggaccgagt tcttcaggtt ttaccgtgat cctgtccgga tctatttcac
     1381 gcggcgggat caccatggga cttccgccgg gaccaaaagc agcaccgggt gctcctgcta
     1441 ctgcggcacc aggagctgat tcttcgtgcg gcggtctata attagcctgt gtcagcaata
     1501 accggacatt caactgaggc tcacccgcat aagatatata gggataggtc acgcgcagcg
     1561 catctacagt aaagtaccgc tgctcctgac gtaaagattc aagaaaacgc acaagatccc
     1621 gggcagtcat ggtaaagttc agccccaccg tctgtagccc cagcatctgc tgatgcgctt
     1681 cgggaaaacg cctgggccat accgctattg tggtcagact gccgatattt gctttcagca
     1741 gaaattccga aatctgaata ccaaactgca gtttgcgaag tgacagatca atttgttctt
     1801 tagtcacatt ctgacctgac cagtcctctt ctttcggcac attcagcatg gcacgaagat
     1861 caagagggaa gcggtcataa ccacccattt tttcgtagac ttttttgtaa agctccataa
     1921 gcatggtatt ggtttcttct gtataccaga acttaaggat gcggtcctcc tgcggtttaa
     1981 tttcaatgtc ataccagccg ccaaaattaa aaaacttggc tcgctcttca cgggcattac
     2041 gccagggctg caccatgttc tgccattcat cgatcagcag ttccggttta tagccgctga
     2101 agttatcttc catttgtttt aaagtcgctt caagagaatc tttcagcgca ctgtcttgtt
     2161 gatatcccgc gaaaagctct ttaaagaaaa agtgccagac accaaaaaaa ccgccaccta
     2221 ttaaggcaag gatcaggaga cccgctattt gcgctttata cttcatacag ttccgtcctc
     2281 cgtcggagtt ggtggggacg gcttatccgc cgtataatga agagcgactt caatggtaaa
     2341 ggtaaaaagg gattcctttc cctgagcgcc tgcaccgccc ggagcaccgc gatctgtaat
     2401 ccctccttct gtgggcgcat cattgagaat actccaggga acgcgtctca cactgccctc
     2461 gctaaaaatc actctgcttg cactcacata ccaagtgttg ggcatctgac gcgaggtagt
     2521 tcgaagccct ttaacaaact cgttgattac ttcatcggat tcggcaaaac catgaatgat
     2581 cacgccattt gccctcggaa tggcgtaatc gttgactttc tcctcggctt gaccgcccgg
     2641 tctcccgcgc cccattccct gttgatcaga agcagccccg ctcgcaccgg gaaactgcag
     2701 ccccggaaac cctcggcgct gtggcgcaac actggatcct tcctcatcag agggcgggcg
     2761 cattgcaggt ccatgctgct gctggggttt ctcttcttcc acgacacagc tttccatgga
     2821 ggaaaaccac atctgaccca gcgtaggccg ggcttcgttg ataaaggcaa attcgtccag
     2881 ccagaagcgt cgctggcgtt ttgctttttc aagcacatcc acctggccga caagggtctg
     2941 aagctgccgc ttacggacat ccagctggga acgaagctcc gattgcggaa caggcacgcc
     3001 cacccggatc cgctgtacca gttcaggatc ataagcacga attgcgctgc gcagttcatc
     3061 gatgcgctgc ttcacccgct tgttttcttt ggcagccgat ggaaccatgg aagcaaaaat
     3121 gagaatcagg gcgacaactg aaagccccca atagagtgcc tgatcttttc ggcgtgccgt
     3181 ttcaagaatg cgtggcggga tcaggttgat attaatcgct acgggttccc agcaccgggc
     3241 agccatcccg agtacaacac aggcctgttc aggggccgct ttaacggacg atgccccttc
     3301 gccgatggaa atgttattga tcaccggtgc aatgcgcaca tccatgccca attgcgaccg
     3361 aaggtaaggc acgatgtttt ttaaacgggc gcagccgccg gtcagaagaa ttctgttgac
     3421 ctgcccgccg ccgggaagtg aacggaaata tgaaaaggaa cgaataattt cggttgataa
     3481 acgctgcagc acctgaccga tcacttcacc gcttttcccg tcacgttggg gatcacctgt
     3541 cggtgcgaag gcacgttcgc gtttgatttt ctccgccgag ataaaatcga gattaaacgc
     3601 ttccgccagc gcacgggtaa tgtcattgcc tccgatattc agaggacggg tgaagcgaaa
     3661 ctgattgccc cgttcaatca caatgtccgt agtggacgcg ccaatatcga tcagggcgac
     3721 acagtctccc tgctgtccca tttctttttg atgaagcagc cagttgtacg cggcaatggg
     3781 caacacatct actgtatcca cagacgcttt tgcagccttg aggatttcga gatgtttttc
     3841 tacaacatca actttgatgg cggccatcat cacatcatag ccgccttgct ccgtcttctg
     3901 cagtacctga taatccattg caatctgatc aagtccgaag gggatctgct gttgaatttc
     3961 atatcggacg atctgattta ctttgaactc cgcaacaggc ggcaatgttc tgcagcgggt
     4021 aaatacggat tgacccggca cacccagcac tgttttccgt cgtcggattt tggattcctt
     4081 cagcgcttca agcaaggcat tgcttcgcaa ttgattacgt gcctgctcct ccagtgaagg
     4141 atcggagttg tactcgcgct ggacgtattt gctgatttca tagcctgttt ttgtctttgc
     4201 cagttcgcat acacgaacgg cactggtgcc aacatcgatc accagccgtt tcttaccggc
     4261 agattttttc aggcctgacc acattctggc catatacgca ccttactttt ttaggcggcg
     4321 tcgaaatgat ctccgcacaa gcttaaccca cattgtcgca cagtgaacat cgggacagtg
     4381 ctttatcagc acaagtaaac tcttgacaaa tattgtagta aatattaatt cagatgtcaa
     4441 gactttaaat gatttttaat tatacaggaa acagatgatt aaaagacaat acttttcccg
     4501 tcttttcaat ccctgccgga aaaccaaaaa gtgccgtttt atttgcgttt tctgtcataa
     4561 cttgcttagc ccgtcaatat caccgttatc cattattttc ttttccctgc atgattgtca
     4621 acaattaatg atatgaaatt tccccggttc agaaaaagaa agtgcacctg gtttgccatt
     4681 acccgctttt tcatattacc atgactctgg ctttgaagaa ttaaaaagca tgtttctatg
     4741 gttgcttccc tcattcattt ccggagacag cgcttcaaag ttgttccatt cttttctacg
     4801 gccctgtccg tgatttcagg agaaggcaat gattttaact gtgtcactct ttgtactgaa
     4861 tgcaacatta tgccagtcgg gcacaattgc ttttatcacc ggtgaatctc ctttcgatta
     4921 tcagctggca tgcctggatc ttggatcggg cgaggtacgg acgcccggcc ggggcctgag
     4981 tgacagtgcg ccccaatggt cgcctgacgg cagcagggtc gcatatcaat ccagaataga
     5041 agggggctat tccgtctgtg tttattctgt ggacacagaa acgactgaga ttcttcctca
     5101 tacatatgcc tggaactatg agcctgcgtg gtcgcccgac agccgcaaga ttgcgtattg
     5161 cagcgaggtc ggggacacac cactgcgttc gctggctgtc tatgatctgg aatcacaacg
     5221 tgaagaactc tggggcggcg ggcacgaagg ccttttgtca cccgcatggc tcccatcaac
     5281 ggatatgctc aaagcactgg atcccgatga tcagttggcg gccgactccc tcggcctctt
     5341 tgctttgaag gaggaggctg aaaaaaatgg tgcccttctt gccatcgggg tggcgggcat
     5401 gccgcccaag ctgagcacgg aaatctttat tgtgacccgc gatcaggtga tccccctttt
     5461 gtctcttctg accggacaga gtacccgtta tgtccggtat gctgtcactc cggactttaa
     5521 aggaaggcaa attgcctatg aatgcaacga gggcggagac cgggagattt tcatgatcgg
     5581 ccgtcgcggc attgcaaact taagcaatca tcccgctgcg gattggaatc ccaaatggtc
     5641 tcctgacaac aactggatta cttttgaatc cttcagaaac ggatacagcg gtatctacct
     5701 gatacaggcg accacagccg gtgttactgc catagcaggc gcaaaagagt atcagtgctg
     5761 gtccccttcc tggtctcctg acggcaaatg gattgtttat gtatcgaacg aaagcggtat
     5821 gccccagctc tatgcctctt ctttcgacgg gataacacaa aaacaattga cgcacgaaaa
     5881 gtatggtgcg ctgaatccgg tatggcgtcc ggctccgcaa aaatcaaagg attgaatact
     5941 atgtgtaact gtagatttgt ttttcttgcc ggatgcttag cattctgcgc gctcacagcg
     6001 caggcgcgaa acacggaagg ggcccttgat cttatccgag tgcccaacaa cggtcagcca
     6061 gtgcaggttc ttcgggaaag cacttttgaa gtgattctgc gggaagcgtg cgagttaagt
     6121 ctcagcggag aaacaggcgt ctatcctatg gcggtgacct ggcatgacaa acgcggtgac
     6181 agggtctccg gcagtgctgt tgttccttct catgtcccca gcggcaccta tgctcttcaa
     6241 ggcgaatgtc aaaacatccg ggatgttaat ttgcgttcag tctacgttgt ggatgcgttt
     6301 ccggatgcct atgcgttagc ccatatcacg gatatgcaca ccggttccgg acggcataaa
     6361 cgatcggatc gtgatattct tatagaactg atacaacagg tcaatgcagc ggaagcgcag
     6421 tttgcactta taacagggga tgtgaccgac gggggcacac cggaagaatt ccggcgtttt
     6481 ctggacattc tcgatacctg ccgtatgcct acttatatca ctcccggcaa tcatgaccgc
     6541 acaggcacgc attacgccga ctattttgag cgattaacct acgctttcca atacgggcgc
     6601 gacggctatc tgtcttttga tacaaaagat tatctgattg ccgatgaaat gggcgtacag
     6661 aatgcacatt tatatttata cagacggcag ctccgcccct gcagatggag tatcggtctt
     6721 acccatcgct atgatccggg gatgggcata catgcccaac tgacgctctt cgtcgatgat
     6781 cctctggatt atcttctggt ggggcacacc catcgggaag cgcgaatgga cgaccagata
     6841 ccctggggaa aaaccgacct tattatgaca ccagcggcaa tcaacggctc ctggcgcctg
     6901 atacgcgttg acagtcaggg gctgcatccg gaagcaacgg tcagtacagt ttctgtcttt
     6961 caggaaaaag acgaactgca ggatgaggaa agagaggatc agtagagata gagtgccgcc
     7021 acttcgttct ggcaggtttc gtagttgcgc cgataaatgg atttaaaggc gctttcactt
     7081 tcctcgcccc gtgcgaggct gcgcagaaaa acgagcattt tgttgcggcc gtagcggttc
     7141 cataacagat ctactgtggc atgcgcctgc agataagccc tccgcaaatc gtcggcactg
     7201 cgatgttcca tctgggcact ttccatggat gccagaggaa gcgccttgtc ttcgttgtaa
     7261 atcgctttca gctgatcgat atcacgggag actaaagatt tcgaaagggt ttccgcgagc
     7321 ccttcattaa gccaccaggg aaggcggtct ccggcaatct gccgcgctac aacatgtaca
     7381 tactcatgaa ctaaccgtct ttttaattca tcttcggcaa tccattcgcc atcggtatct
     7441 gtaagagggc tccggatttt tccgtcatat accgcaccta tatgcccctg catacgggtc
     7501 gcttctccga attgctcaaa agaataaagc actacctgca caggcgacgg cggataggta
     7561 ccgcccagcg ctctgccaac atcaaggtac gctctctcca gaatcgtcgt gattgcccct
     7621 ctcagccgtg ccggtgtatc tttaggataa ttgataatga aatgattggc tttccatcga
     7681 ttgaactcct gttcgaccga ttcttcccga aatgcctttt cataacggga cgccagccct
     7741 ggtctttccg gatctacggc aagtacataa tcccattgca cccgggcaga atataagtcg
     7801 ttgtcttcat aataggcctg cccgagcagt tcatgagcca tgaggttccc cggtgccagc
     7861 ccgatagcct cctccagcgc atcgatggca tcggcgactt tgtcctgcga aaggtaatag
     7921 gcgcccatct gcatgaaggg agaagcgttt tccgcatcaa cttttacagc cttgcgcagg
     7981 atctgagtcg ccagggtgaa ctcccgttcc gccgccagca attccgcttt tttttgatag
     8041 gcgttacaaa gattccggcg gatagcagta ttggagtagt ccagtgcaag cgcctcttcc
     8101 agcagcatga ccgcccggga aaaatcgccc cgattaaacg catccacccc ttcattattc
     8161 attacagtcg cttcctgagc atgcagcgga gcaccggcaa gacacaggaa aagaaccacg
     8221 cccacaaact gtaaaaaacc gcgtctcatt atttttcttc cggaaaagcg acgattgcct
     8281 ctatttccac ctgcataccc agcggcaaac cggccgctgc tatgcaggtc cgtgcagggc
     8341 agtcccgtgt aaaatattct ttatacaggg tattgaaccg tgaaaagtct gccatatccc
     8401 ggagaaagac tgtagttttc accacatttt ccaaaccaag accggcatcc tgcagaataa
     8461 ccttcaggtt ttccagagcg ttacggcttt cttcctcaat tgttccggca acataaccgc
     8521 tgccatcccg ggcaagcgac ccctgcccgg atataaacaa cagatttcct gcccgtacgg
     8581 cagcagaata aggcccggca gcaggcggtc cgttgggcga tacaatacac tgtttcatct
     8641 tgcttttcct tattatttcg tgccagtcat catagcagga ggaggggaat catcacaacg
     8701 ctgacagctc gggcttcatt tactcaaaaa aaggccgttt ctgaaatatt tattcctcta
     8761 cggtcgtctt gccggaccat ctcaggactt cattcctgcc gctgccatag cggtgatgtg
     8821 ccgcccgggc tccccgatgg cgttcataaa ccaatacacg gcggcagctg tgcccgtgca
     8881 cacgcatgtc cagtgtatgg gagcggcagg tgcgtacata aaagggcatg tacagttctg
     8941 ccagccagta cctgaaagaa gaacattgca ggccgaaatt ttttaacagc tccgggtcat
     9001 ccagcagaaa agccagacgg gcaaggtcat atccttctct gtaatgcctg gcgcattctt
     9061 catcaaaatt tgatgcccgc cagataaaag cctgaacacc cggtaatcgc aggggaacac
     9121 aacgcttttc tatcagcttc cgcatcagaa gaatatcggc cgcacgcata gcttcgaaat
     9181 cttccggaaa tcctttctct tccagaaaaa ggccgcggga caaactgcag tggcggctgg
     9241 aaagagtgta gagagactcg ggcttctcca cattcatcag agcgtgatcc tgtttcacaa
     9301 accagcgggt cagcagtccg cctggaagcg cactgtgagg cgccgactga ccgaaggcaa
     9361 tcaccggttt gcccgtagtg gcgtgtatct ccacatgacg ctccagcaac tccggtcctg
     9421 ccagcaaatc cggatcgaga aaaagcagca aagcgcctga agctttgcgc gctcccacat
     9481 tctttgcctt cacctcactt gaagagggct cgtgataatg tttaacggaa atagaggcgc
     9541 cggtcagaca ctttgcaatg cgtgccattg cgtccggctg cgcttccccg taggacacaa
     9601 gaataagttc ataccgtgcc gtcggcatgc tctgctgttc aaaacaggtc aataatgtgg
     9661 gcagtgcatc tgttatgtct tttgccacaa cgattacact gatctcaacg tttgccatct
     9721 tttccattcc acatcttgtg cttcgaatca ccggactgca ggcaacagtc taagacacaa
     9781 gcaaattcca cggattatgg tatcacaaaa caagataacg aaaagcagga atagagaagc
     9841 gggatccggc gattagtgcg tcagatgccg gtgtccatca tccgcttctg agcaaaggcg
     9901 tcctccgcct ttgcagcaca acgattcgcc ccgggcacct cgcgtcgggc ctttgcagcc
     9961 ctgtacacgg taccccagtc ttttttctgc cagcagctgg gtgccggtcc agagaccgaa
    10021 gataatcgcc ataaacaaga gtgcgaccag aggtgttatt aaaacagcag ccatgtttca
    10081 gccctttaaa agtcacggtt ttatgtcctg ttatgagaac atttttcgga gaaaaagtgt
    10141 tccagcctca tcggatcgtt tcgtcagggc atttttctcc ttctgtatta tcagcttcgc
    10201 tttgagggcg tgtgcttttt tccagccggg taagcgtata ctccaattcc cggactctgc
    10261 tgcgtatatc ttccagcagc tgccggttca cacctatcag gtcggcgatc agccccacga
    10321 ccatgagcgc cagccctgtt cccaggagaa gcgcactgag cacaacagac tgaatatgcc
    10381 ctgctccatc gcccaaccag tataaaagga ggaaacgcag gagcagcaaa gatcctgcac
    10441 caaaaacaag cagcccgggc atggcaaaga agcggaacgg ccgataagtc ataaaaatcc
    10501 gaacaacagt aaaaagcgac ttgcggacat attcggggat gctcttcatc agccgggacg
    10561 gacgcaactc atcattggtt cggataggca ccgacatgac gggaatgcct ttgcgccctg
    10621 cctgtatgat ggtttccagt gtgtaggtgt aattatcgaa aacattcagc cgcacggcag
    10681 cttctctgct gatggcacgg aatccgctcg gcgcatcggc aatttcagtg ccgcttgcca
    10741 ggcgcactac ttttgagcct attttctgca gaattttttt ctggaaagac cagtggcgga
    10801 tgtcatctat aggccgtgct ccgatcacca tatccgcctc acctgtcagt atgggatgaa
    10861 tcaatttctg aatatcatct gcacaatact ggttgtcggc atcggtgttt acaatgatat
    10921 cggcgccacg ctgcagacag gcattgagac cgtgcagaaa agtatgagca agccctctat
    10981 tgcgcctgtt tctgacaacg cagtgaaccc ccttgcgaat ggcaagatca acggtgccgt
    11041 ccgtggaacc gtcgtctata acaagccatt caaccgagtc aacgccttcc agcgtacgag
    11101 gcagcgcatc aagggtaata cccagcgtct tttcttcgtt gtaacagggt atttgaataa
    11161 tgaccttcat ggcagcaaac ctcagggttg aaatgcacaa gatggcggcg atgccatcat
    11221 ctcaaaaccg ccgggctttt aagcagacaa tcctttaatc gggtaccttc gttcatcagt
    11281 ctgaaacaaa agagatcagg gaaggggcac cttgatctct tttccaagga caattaggtt
    11341 ggcgtcctgg atgccgttga ttgccattaa gttttgcact gttgtgttgt agcgctttgc
    11401 gatggaatgc agcgtatcgc ccacttgaat cacataagag ccgaattccg gtgtcgctgt
    11461 atcttcagtc tgcgatgaag cctctttatt ttcatccgca ctgttctccg cctgcacggg
    11521 agcgtcatcc ggtgcctgag tatactctac aggaggactc acgggaggga tcatactttc
    11581 cggacgctcg tcctgttttt catcggaggg ctccagcagg tctgaagcag gctgaaagtc
    11641 ggtctgtgaa tccagatcgc tgattacatc cgtcgcttct tcaggaagca cttcatcagc
    11701 cgaattcttt tcggtttctc tggaaggtgt cagcggaggg ggtatttctt cagcttcagg
    11761 taccgaactg gtatcctcgg ccgttacggg ttcagtttcc acctgctctg tcgtaaccgc
    11821 ttcaagcgct gctgctgttt cgggggaatt acccgccgtc tcctgcaccg gagcagcctc
    11881 cgcaatacct tctgattctg tttcggctgc agcagcatga tccgggctga taatttctgt
    11941 caccacatct tcaaccgttt tttccacaga aacttctgca ccggacggtt gactctgctc
    12001 cggcgcattt cctgcctcgg gcacatagat ggttaatttt tttccgaatt tgatgtctcc
    12061 atttgtcagc ccgttccatt tcgtcagatc tgaggaagaa acctgatggg ttgtggcgat
    12121 tttacttaaa gtatcaccgg gctgtatggt gtaggaaagc attttggtgc cttgcggctg
    12181 gggactgggg gtatcctttt tcgtctcctg tacaacagct ttctcttgag gtgcggcggg
    12241 tttctcctcc tgcggcggtg cggtttcttc gatcacaggc accgcttcct gcaccgcagc
    12301 cggagttgct tcgacctctt cgggcgattt cgcttggggc tgcttttctt ccaccagttc
    12361 ggggactgcc tcctgatcaa taatggttga acgaggcgga gccgtctctg aagagggcgc
    12421 aggcgtttcc tctgcacttt ctgcggtttc tttttccgcc ggagcggttt ttaccgtttc
    12481 aggcacaaca tccgcttccg gcactgcagg ggcgctttcc ggaatcgttt ctgtcacatc
    12541 gtgaggctcc ccctccacct ccgccgggac tgcagaacgc tggcgggcaa tttgttgacg
    12601 ccagctgtcc agacgtcgcg ccgaagcatc aagttcactc ttattcaaaa tgccccgatc
    12661 cgccgcccgc ataacttcct gagcaatacg cccgagttgc tctgtatttg cagatgaaat
    12721 caggacagca tcacagcccg ctttcagcgc atcgacaaga tcagtttccg gctcgttacc
    12781 ggaccagacg cctgcctcga taatgtcgga ggcaagcact acaccggtat agccgaactg
    12841 acgtcgcaac acttcacgaa ccagccgggg tgataaggaa gcgggacggt cggggcgttc
    12901 cttatccaga gccggcacag aaacatttcc tacgagtatc ccggaaatat cataggcggc
    12961 agcctcggcg aaaggcagca tggcctccgc cagtttttca atatccgttt cttccacaac
    13021 cggaagcctt cgcggttgat gcacggcgct gcccatgccc ggataactgc ttacgactga
    13081 cagtgcgccc gcctccatga gccctccggc ataggcaagc cccgcctgaa tcaccgattc
    13141 cttattgtcg ccgaaataat gcgggcggtc ttcttcacgg cttcggccgg gaaaaaaaag
    13201 atccaggcga ggggcaagca atacacccac gccggcactc aaagccgttt ccgccacctg
    13261 gcgccccgcc tttttgatgg catccaggct gtccagcgcc gcaagttcct ggcaggtcag
    13321 tgcgccgtct atctgaagca ggtttccggt ctgaccgcct tcctgagcca tgcagataag
    13381 cggatttttc tcgcgggaac cgtcgagcga tgcatagaga ttgatgagcg aaatcatctc
    13441 ttcgatctga cgggggtttt ccgtattgtt ttcacgcagc cagaccccgg cgggcttcca
    13501 ggcattcagc atttcgatgg ttttgcggga gagatgggtg ccttccacac cgacaatcac
    13561 atgacgcaaa ggccacatgc tgtccatgtc tgcagggatc ggctcaatgg catactccgg
    13621 ctgtacgggg gggacaactt cagggacgac ggcttgagca gggatcaccg gttcaggaga
    13681 agtatcaact tgtgccgtcg gctgctgttc ctgcactttt tcagaataga tttcggaata
    13741 ggcataatac agacaggcac ccacaattgc gccaagcgtg aacgtgacaa gttgactgaa
    13801 aagggaagaa cgaaaccagg attttctacg ggtggcgcgc ctgccatatt gactaatcat
    13861 aatttaatag aaaccttacg acagaaaaat gtgtattttc agcagaaaaa gttgtccaac
    13921 aaatcttatt gtataccaag ggatggctcc ggtgcatatc tgcggctcac aatgtcttca
    13981 atcagtgcct ggtactgctg cctcagaatg cggtttcctc tttcattgag atgacagtgg
    14041 tcactgaaaa gagtttctcc cggtatgccg tgcggctctg ccgaggcaac agcagtttca
    14101 acatcagcca acggcactcc gtatatccgg gccagatcac ggatgatgcc gttttcaact
    14161 ggagacgatt ggtgacgcgg agaggcctgg gcaaggatct cagcaccgcg ccgtttcgct
    14221 tcgtcaaagt cgcccgcctc atatagctgc accacttcct cataaagctg cccttctttt
    14281 cccggcagat tgggtctgat cagattggac ggcacggtac cgataatcag cggtacttcc
    14341 gcttctctgc actgggcaat aatcatggtc aggttctgcc ggtatgcctc catacgcccg
    14401 tcgatttctt cctgagtgaa caccatttcc caggtttttg aggagtcagg gatagactgc
    14461 gccagatccc gtgcctgcct gctctcttcg agccgggcaa tagcttttct tgcgcgcatc
    14521 tcccgtatca gacggtagaa cgccgatttc cccagatact gctgcacttt tccataattt
    14581 ttcaacgaga gatgcagctg ctgcaattct tcgaattcgt tgtgaccgct gtacagcaga
    14641 acaatatcag gctgataatg aagaatttcc gccgcaatca gcacaagcct gtggcttcca
    14701 taggagagcc cgccgcaatt gatcacctca acagcatcga cagacgcatg aaagcggtgt
    14761 tccagttctt tttccaactc cgaaaactcg taatccaaat aattaactga agaaccgccc
    14821 aaagcgaaga tacgcagtac gcccggcggt ttgggcattt taaaacgctg ctgtttaaaa
    14881 gacaccgttt tttcaggacg ggtttccatc cacccgcctg gtacaggcgc aaaaagcagg
    14941 ctgctttcgt caaatccctg cccgatatcg acctgcagca ccggatgagc ggattcataa
    15001 aaacgggcga taatttccag accgccgaag aaaagcaaaa gaggcaggca aattaaaagg
    15061 aactgaaaca gacctgagaa aggtcttccg ggcagggaat tgttttcgga ttttgctaca
    15121 ttcacatcaa ttccgcacct ttacttttcc cgtctgaaaa aactttaggc gttgcaggca
    15181 ccatgatcca agaaactgcg atgtccttgt ggtgaaagta tttttagcgt cggctttatt
    15241 ccagatgttt caacgctttc gcccctatat cacggcgata aaaagcgccc tcaaaatgga
    15301 tgcagctgac ggcctgatac gccttttcca tggcggcacg ctgtgtattg ccgagcgccg
    15361 tgacatttag cacgcggccg ccgctgctga aaagctgatc tcctttccgt ttcacgccgg
    15421 cagcgaacac agcaacgtct tccagcgcct ccgcttcatc tacgccggtg atttctttcg
    15481 ctttggcata gacgccggga tatccttcgc ttgccatgac tacggaaacg caagggcggg
    15541 gggaataacg gatttcgcag ctgtccagtg tcccgttgca gcatgccagc aatgccggaa
    15601 ccagatccgt ttccatgcgc ggcaggatcg cctgcgtttc cggatcgcca aaacgcacgt
    15661 tatattcaat gactttcggg ccgtttcccg tgatcatcag accggcgtaa agaatccctt
    15721 tatagggcat gccttctttc gccattcctg ccgatgtggg caccagaaca tcccagacaa
    15781 tctcttccat cagtgcatcg gtcacgacag gcgccggaga ataggcgccc atgccgcctg
    15841 tattcggtcc ttcatcgttt tccccgactg gtttatgatc ctgactggaa gccagcggtt
    15901 tcactgttct accatcacaa aaggcaagaa tggaagcctc ttctcccgtc aggtattctt
    15961 ccactaccac ccgggcgcct gcttcgccga aagcaccttc agccatggcg gcatatattg
    16021 cggcttccgc ctctgcctga gaaacagcaa ctgtaacacc ttttccggca gccagcccgt
    16081 ccgctttgat tacaatcggc gcaggctgtg agcggacata tgccagcgct tcctgcagat
    16141 cgtcaaaggt gcggctttcc gctgtgggaa ttccgtgacg cgccatgaac tctttggcaa
    16201 agctcttact tccttccagc tgagcggctt tttttgaggg gccgaaaacc cgtttgcccc
    16261 gttcctcaag aaaatcgact atgccatgca ccagaggcac ttcagggccg accacagtca
    16321 ggtcaatgcc ttctgcttca atgtaggtat ccagcgcctc aaaatcatta acgcccacat
    16381 tgatgcatgc ccctttttca agcctgtcaa taccgggatt gcccggtgca ccgtacactt
    16441 tttccaccag cgggctctga gcaatcttcc acgcaagcgc atgctcgcgt ccaccgctgc
    16501 caatcaccag cacgttcatg gtatcgctcc tcagacaaac cggaagcaca ccggtttctg
    16561 atcagttaag aatttttatg atttctgatt tagttgaacc gaccggacag accggccgga
    16621 cagaatacag cctgtaaaaa gaaataaatc gtgtatctac ctgaaatctg ccggaaaccc
    16681 aaccggcact cccgtgcatc agtatacgaa aaacgagggg gtcggaacga acaggcaggg
    16741 tatctccttc caattaaaga agatcgccgg caagcagcag cgcccccacc actaccacat
    16801 cttcctcaag ctcacaggct acaaactgca ctgtatcgtt aaaaggcgca aaaatgcggg
    16861 cagagagcgc cgaacgcagc ggcttcagca gcacatcgcc cataagcgcc acaccaccgc
    16921 caagagcgat acgttccgga tgaagcaggg tcaccacatt accgagagcc atggctgcag
    16981 cttccgccac atgcccgatc tcatccaaag cagcctgatc ccctgcctgt gccgcctgtg
    17041 ccagcatggg gcagctcaaa agagcggcat cgccgtcgca taatgcatga agcggggttc
    17101 ccggttccgg cagcatcgtg ccgcgtatgc gcctttcaat tgcccagcca gaacatagat
    17161 tttcaagctt atccgccgcg cccggttggg cagatgtcca gtccggaacc caggtatgcc
    17221 ctatttcaca ggcaccgagg ccctgtccgt cgtacagttt cccgtcaaca atcagtgcac
    17281 cgccgatacc gctgcctatg ttcatgtaca caaacgtacg ggttccccgt ccagcaccgc
    17341 agcaatattc agcccagcct gccgcattgg catcattggc tacagccacg ggcaggtgaa
    17401 accattcttc aaaacagtgt ttcagcggcg cattctgcca gccttctatc tggtgcgata
    17461 caagcactct tcccgtgctg ctttccacgg gaccgccgaa accgacccct attgcctgca
    17521 cggcttcttt tgctgaagcc agcgccagca gttcgccgca ctgctcccgc atccatcgga
    17581 gaataccttc cgcaccggct tccggattca cctgaccgcg ccgacgctcg agaatcgtgc
    17641 cgtcggacag ccccagaacc gcctgcagct tagtgccgcc gatttcaact gccatgagag
    17701 atttcttttg gtgattcatt tcagatcctt ccagtccggc agggaaatca gcaggatttc
    17761 ccgggcgccg aattttgacg aggaaaaaac aattgcacga ctatccggcg taaaactggc
    17821 atgagggtgt gttttaaaat cgccggaagt atgtcccgca gtcagcagcc tgcgttcttt
    17881 ttcctccccg ttgatcagcc agagagaacg atcaaaatca tctccgagca gccatttacc
    17941 atcggggctg ccatgggtgt gccaggcagg ataacgcact atttctgtca tttttccctg
    18001 cggtccgctt tcaagatccg ccatcatgat cccatgaggc ttgttttttc gttcttcatc
    18061 ataaggccag acagtgaaaa gaaaacggtc ctgcccccac cagcattcgt gcgtgaccca
    18121 ttcccagccc tcctcttgat agagcggttt catcgctgag ccgccatcct gaatatgcca
    18181 gatgcgctga ttagcgtcgc cacctgtttc ctggcagaac atcagggaac ccgggcgata
    18241 cgggtttgcc tgaaagtgtc cgatacgaaa atcagttttg cataagagac ggctctgacc
    18301 gtttttcaag gataaaacat agatgcccca ctccccttcc gaaaccagag aagcgccgaa
    18361 atagagcgct tcacccgaag catccacggt aagtgccccg gcaagccggg cgcattccgg
    18421 cggcaaagca cctaatacac ggggctctcc tctgccctga aaggaatcaa cgacgtccgc
    18481 ttcgtgaaaa taaccatctg caaaataata aaggctgcgg ctgcgccacg ccatggcggc
    18541 aaagtgcaca ttgccgtcca gaagcagccg gctctctcct gttctgattt ccaaaagatg
    18601 aggatgtagg ccgccctgtt ccggtcgtgc catgaagaga aaataatcca tctccggagt
    18661 ccacatggga tgggtctgat agaccacttc actctccgga accccgcgga caagaacaac
    18721 gccttccaca ccggtcactt cgtcctgcca ccggggatat atgccgtcgg gcagccagtg
    18781 cagtttttca gcggcacaca gtttttttgc agtattacgg ggcatgaaca tagtcgctcc
    18841 ggctgctacg gtaccggcca aaaaagaacg gcggctgacg gtatgttttt caaacgctct
    18901 catgcgagcc tttcacagta caaaaacagt ttcggagggt attctgttca aatccgggtt
    18961 aaaagtcaat ctgagcgatg caggcattca agggtgcgca gaaaagcccg ttcggcggag
    19021 agcacacaat cattcaggcc gatgcctctg taggcatttc cggcaaaaga cagtccgggg
    19081 tgtctgcttt ctgcagcctc aatggcttcc agccgctgct catgtccgat tgtatattga
    19141 ggaattcctt tttcgtggcg tttaatccgg atgaattcgg cttttccccc tatatccagc
    19201 acaggcgtca gttctttaag tacaaggtct gccaattctt catcgctgag atccagcact
    19261 tccggatcgg tagagccacc tgccatgact cgtaaaagcg ccatattttc cggcgcctga
    19321 cggggaaata aggtcgatgt ccagaggcag ccgagggtgc gaatgccttc gccacggggc
    19381 acaagaaagc cgaagccgtc cagatccctg ccgatagccg ctttgggaaa cgccagtgtc
    19441 accacagcaa gccccgcata aggaatggcg ccgagcgccc cggagagggc ggcatcaaaa
    19501 tccgacgtaa aggcggcagc ggcataggca ggcacggcgg atacaactgc acgacaacga
    19561 ataacggagc catccgggag gatcacttca taaccattgt ccccgcgggc aagcgccgaa
    19621 acaggggtgt taaatcgaat gcgatcctga agctgtgcgg aagcgccttc aatgagggtg
    19681 cctgtgccgc cctgaaagga ggtcagcgtt ccgggcgggc ccatagggct tgcttttctt
    19741 tttcggcgga tagcgagcag ggccttcatc agtccgccat aatcccgctc catggcgacc
    19801 attctcggaa aacaatgcgt catactcagt ttttccgcat cgcccccgta tatgccgagc
    19861 accatggcac ccaccattat ccgtgccgct tccctgccga ttcttctgga agcaaagccg
    19921 tagatcgttt ccggactatt gtcctttttg ggggggataa acggctccag gcacaggcgc
    19981 aagcgtccca tgacggagag aaggggcgaa aagaaaaatc cgggcggcgc tttaatttca
    20041 tgaagaacac cgttccggca aataaagcgt ttggctgccg ccgtatttgc ccgcaccagc
    20101 tgattcgtga gccccagttg ctcgatccat tccagggtac gcggctcccg atccagaaag
    20161 ccattgcctc cccagtccat cacaaaacgc ccgtctctgt cgctgcctgt cgttccgccc
    20221 ggatgcggtg ctttttccag acagagcaca gcttctctgc caagtttttg cgccaataaa
    20281 gtagcggtgc acaaaccggt tactccggca ccaatgacaa caacctctgt atccataaaa
    20341 actttccatg tcatgcacaa gaagggaaat acatccggcg ttgaatacgt catttccgga
    20401 cagaacaagc atactactgc agcccgcctt cttccaagcg cgacacgccc gagccggaat
    20461 ttttctctgc taaatattgc atcctctccg tttttcaagg ataataccgt tttatcctaa
    20521 tccctccggt tgtgtccgtt cagggacttc cggaagtcat gtctactgct tccacaggat
    20581 gcttcatgtt tttcacttct ttgtttctac ttttcgggaa cagcctcatg tttgccacta
    20641 ctcaggatct catgttcagt cccgcttttt acgaaacagg tactggatgc atctggacac
    20701 gtccgcattc cgaacttctt tccatgaatg aatggttcct cgccggacct gctcccggca
    20761 tataccgtga cgaatggctg gaagccgctg cggactaccg cgaccaggtc agccagggcg
    20821 gcaaacgatt tttactgctg gacactgccc gcggagtcgc ccgtgtacag ccttcttcag
    20881 cactgacccg gctgctgaaa ctccagccgg gggacgaact tttttactct ttccgtgcac
    20941 ggagcggaaa agaatctgta ttttctctaa ccctggaaga aatcacccac gagcaggggt
    21001 cgccggacaa ctggcagaag acattgcaga cactgcacct tcccggagac ggggaatggc
    21061 atgagctttc ctggtctgta aacatccctg aagacgttcc ggaaacctgt atcgcagccc
    21121 ctgtcctgac tctgatgccc ggctctgaca tgtccgcttc cgcggaaatc gccggcattc
    21181 tcttcggcat cggtgacacg gagcggatgt ccctgctggg gaatcacttg cgtttcatga
    21241 acagcaccca tatgtgccgg tgcatttaca accgtgacga tctggaatgg gcaggcagtg
    21301 ccttttccgg ttattttctt ttcgcctatg accgccggtt ctacaccccg gaagagggat
    21361 acaaaaccca ggcactgatt gaggattttc aggaacggct gggcggggtg gatactgtag
    21421 tcttctggcc ttcttatccg cagctgggtg tggatgaccg cagtcagttt gatttcttcg
    21481 acgatatgcc cggcggtttg acagggctgc gggagcagtt ccgtctgatg caggaaaagg
    21541 gcatccgcat tctcctgcct ttcctgccct gggacacggg cacccgtcgc gaaaatgatg
    21601 aagcggaaaa gcagctgatc gcactcgttg cggctctgga agcggacgga atcttcatgg
    21661 acagcctgtc cggtgcagaa gaccggctcc ggatggcgac cgactcggaa aaactgggcg
    21721 tggtttttgt tcccggcgca tgcccccctg tggagcaggt gagtgtatgc aatctgtcct
    21781 gggcgcaaaa tgcggagtat cccattcctc cgggaatccc tttgctgaaa tgggtggaac
    21841 cacggcacat gcagcatttc gtgcgccctt tcagcctgga tcggcgtgaa gaaatcgagc
    21901 aggctttttt caacggatcc ggtctgatga tctgggaaaa tgttttcggc tttgccaatc
    21961 ctctgtccga agaggatgcc gcactatgga aacgctgtgt atccattttg cgaaacatgc
    22021 ataagccttt cacatcctcc ctttgggatc cttttttccc gtcccttcag gaagacctct
    22081 atattcaccg ctggccgggt gcgccgggca ctgtcttcac gctgatcaat aaaggggagc
    22141 ctctggaata tgtgcctctt atccgatgga aacttcctga atctctgcgt gccgatcaga
    22201 tggtcgtcta tgatctgtgg aatggcggta ctctccgctg ggatatgaac gaattcggtg
    22261 ccgttcaggt atggggcgac gtggagcgtc tgggctgtat cgccatcagt ttttcgcctg
    22321 acgaaaatct tgaaaatctg gcggcacagc ggaaaaaaga acagcttccg gaggcatcat
    22381 cccgccatca atacctcgca cttccgcagc gtctcgcagc gccggcagcg aatgcatctt
    22441 caaacacggg cggaacagac ggaatgcggt ttatctccgg tggcggcatt acgctggcag
    22501 cggcaaatcc gggcaatccg gcagatgagc gtctgcttcc tttacagaaa caggcggagg
    22561 cagaaacgca tcaggtcaat cctttttata tggacgaggc acaggtcagc aacggagcgt
    22621 tccaagtgtt tcttcaggaa acgggatatc atcctgaaaa tgatcagaat ttcctgaaac
    22681 attggggcgg ccgttccatg ccggatgcgc tggcggatct tcctgtcgtc tatgtttccc
    22741 tcgaagatgc ccgggcatat gcagcctggg cgggcaaacg ccttccgacg gaagaagaat
    22801 ggcaggcagc cgctcaggga gaggacggac gctgctggcc gtggggcaac gatttcagca
    22861 gtcagcactg cgccccggcg ggtacagcgc ctgtatcggt tcgcagttgt gcgtcaggca
    22921 gaagccctta cggcatgtac catatgaccg gcaatgtgcg cgaatggacg gaaagcgaat
    22981 acagcgacgg aatcacccgt ttctgtatac tccggggcgg ctcctgctat gaggcaaaag
    23041 gcagccggcg ttatgttccg ggcggaccgc agccggtaac agcccgcaca aaatatctgc
    23101 tgatgcatcc cggcctcgac cggtcgtcgc tgatcgggtt ccgctgtgcg aaagatgcgg
    23161 tcagcgccgg ctgaagaagg aaaattttcc gagagcaacg ctctccgacg gcttgtaatt
    23221 tttacaattt gactgctaca ctcttcaaca atggaggaca caccgtgaac gtgcagaaag
    23281 ctgtaattac cgccgccggt cgtggagtcc ggctgtttcc cgtagcagat accgtgcaga
    23341 aaggcatgct gccgctgcag gacagagacg gtctagtcaa acctgtgatt cagatcatca
    23401 tcgaggaagc gctggaaagc ggcatagaag aactgtgtat tgtctgtgct ccaggggacg
    23461 aaagccgcta taggcaacaa atgcagcaga tccgcgacaa tctcctggaa ggctactcag
    23521 gggagcagtg ggcgcagcag caggcggcaa ggctggaagt tatcctgaac aaactgtctt
    23581 ttgccgttca ggaagaggcg ctgggctacg gtcatgcggt ttactgcgcc aaagactttg
    23641 caggggatgc tccttttctg ctgctgctcg gcgatcacct ttatgtctct catcttgaag
    23701 ggaaacgctg tgctcagcag ctgctggcgc tggctgcaca ggaacaatgt gccgttgcgg
    23761 cagttaaccc cacgagagaa catctcgtcg gcaattacgg tacagtcagc ggtaagcgcg
    23821 tccctgccat gcccggcgtg tacacccttg aaaagatcat cgaaaaaccc tcagtcagcc
    23881 tggcggaatc agttctgcaa acccccggtc tccgggcagg tcattatctg tgctttttcg
    23941 ggatgcatgt actcacaccg gggattttcc cccttattga agaagaaatg gcgcagtcga
    24001 aacgcagcaa cagcagctgc cagctgactc cggctcttca ggcgctggca ttgcgagaga
    24061 agtatctggc tacagaggtt cagggaagtc gctatgatat cggcgcccgc tacggactgc
    24121 ttctggctca aatggcgctg ggactcgccg gacaggatcg ggacgtcatg ctctccgatg
    24181 ttgtgtctct tctggcggaa tgtcgctgca acagcagccc ttcgatggaa agacaggtat
    24241 gaacgctttt atcgagacca tcaccagccg tgacgaagtt ctgcgcaacc gcccctttcg
    24301 caccctgtgc gccggcagaa attctgctga acttctggcg gcggcacggg aactggaagc
    24361 cttccggcag tcttcagaaa atctttacga gcgggtgcgg gcatctcttt ttctctacgc
    24421 ccttcaccgt ttttacctga gtgaatcggc agacttcccc gcttcgggca cactcccgcc
    24481 ttccggtttc tatgatctca tggagcgacg ttttgaagaa gcacttgcct gcttctggaa
    24541 agaggtgaat gagcagggcg ctacaggagc actttgcagt gcgctggcgg aaagctatca
    24601 ccatctctct tttgacacgc tgagcgatca ggtgcggcgc agcgtgcgct cgagccgtgg
    24661 caatcagtgg atgttccggg tcggtcaccc tgcagaacat cccctccgct gtcgccctga
    24721 gctgcagcgc cgcgcctcag gagaaagcct ctaccctgtc cttgttgagc ggacaccagt
    24781 ccggctggat ctaagccaca gcggatggtc cgacattttc tttctcggca tggactaccc
    24841 tgaaggggca cgcgtgctca atatcagcgt ggatctgggc gtatacggtc gcgacagcag
    24901 cacccgccct ccggtacagg cctatgcccg ggtcatcacc gaaccggttc ttcgcatcac
    24961 cagtcttgat ctgggaacaa gcaaagacat ccgggatctg ggtgatctct tcaatttcgg
    25021 caatgactat ctgagccttt taaaagccgg gatcatcgcc tccggcttta tcccgccttc
    25081 ttttgaaggc acggacatac ctctggccgg gattctgggt gaggttatcc gccccggcat
    25141 gggaatggaa cttgtcactc aggtgaacga tatccccaaa ggatcccgcc tcgctgtttc
    25201 cacaaacctg ctcgcctgtg tcattgcgct gctcatgcgc gccactggcc aaacacagtc
    25261 cctcacaggt tcacttacag aagaggaacg gcgccttgcc gcatcccgtg ccattctggg
    25321 cgaatggctg ggcgcttcag gcggaggctg gcaggattca ggcggcatat ggccgggcat
    25381 caaagtgatc cgcggcgccc ttgcgggaga atccgatcct gaacacggta taagcaaggg
    25441 gcggctcctg cctgagcatc agattctggg cgatcaggat ctgcatccgg acattaccga
    25501 acggctggcg gcatcgctcg tgttaattca cggcggcatg gcgcagaacg taggacccat
    25561 tcttgaaatg gttaccgaaa aatatctgct ccgctcccag cccgaatggg acgcccgcct
    25621 gcggatggac aagattttca acggcatccg cagtgccctg aaaacaggag acattaaagc
    25681 gctgggcgcc tgcaccacac aaaactggaa tgatccgctc aaaaccatta tcccctgggt
    25741 cagcaatcat ttcacggaaa gcatcatcag ccgcgcacgg gcggcacttg gggacgattt
    25801 ctggggcttt ctcatgctgg gcggcatgtc cggcggcggt atgtgcatga tggtggcgcc
    25861 tgaacggcgg gaagccttcc gtgacattat tcttgacatc atgcagtcaa caaaagagga
    25921 gctggaagaa gccctgccct ttgccatgaa tcctgtcgtc tatgatttta cgatcaacaa
    25981 tcagggaaca caggcggcgc tgctgaccgg agaagaagca ctgcttccca tgtcctatca
    26041 catactgctc accccggatc ttgcccgaaa ctgcccggag aatctcccgt tgcagcggcg
    26101 gatagaactg gattgcgcca tcgccgcctg cccagaaccg gataaacggg atcaattgct
    26161 tcgaacactt gtgggcagcc tgttccgtgt aggagatgcg ctgtcacgaa gcgagaagaa
    26221 ccaatgggac gaagaagcgg aagcgatcaa ggcacagaac ggctttgaca gcattcagca
    26281 tgaaatgatc cgggatgatc tgcggggggg ccgcatcggg ctttcccgga accgcctgcc
    26341 cgccgatacg ctcatcgaag atgttcagga tcaggatatc ctatttctta aagccgatgc
    26401 ccgaagcaga cagtcgggcg aagaagcgct cagagcgggc accgttgctg tgctgtcact
    26461 ggctgccggc gtaggcagcc gctggacaac aggtgctggc gtaatcaaag caatcaaccc
    26521 ctttattttt atggcaggca ggcatcgcag ctttctggaa ctccatctgg caaaaacacg
    26581 ggaaagcatg cagcggtaca gctgcgtaat ccctcatata gtgtccacca gttttctcac
    26641 ccatgggcct gtcgtaaagc atctggagca acagaaacgc tatcagcatc caggccccct
    26701 ttacctgtcg ccgggcagat ccatcggaca gcgttttgtg cctatggtgc gggatcttct
    26761 ttttctctgg gaagaaacga ctcaggaaag cctggatgaa cagaaacaga aggtgcgcga
    26821 tgccgtccgg gcagcactga tcgactgggc gaaagccaaa ggggaaggaa cggactatac
    26881 ggacaacctg cctatccagc ggtttaatcc gcccggccac tggtacgagg tccccaatct
    26941 gctgctgaac ggcgtactgg cgcagttgct gcaggatcag cctcagatca aaaccctcct
    27001 gctgcacaat atcgacacgc tcggtgccga tctggatgca actgctctgg gcatgcatct
    27061 tctgtcgggc aatgcgctca gctttgaggt tacaccccgg cgtattgcgg atcggggcgg
    27121 cggtcttgcg cgggtaaacg gaagactcag gctgctggaa gggctggcac agccccggga
    27181 agaagatgaa ctccggctgc gcttctacaa tacgatgacc acctggatcg atgtcgatgc
    27241 gctattgcag cagttcgggc tggtgcgcga agatttatgc cggtcacggg ataaagtcgc
    27301 tcaggctgta cgcatgatgg caaggcgcat gcccagttat gtcacgatta aagatgtgaa
    27361 acgacgctgg ggccgcgggc aggaagatgt ttacccggtt gctcaggtgg aaaaactctg
    27421 gagcgacatg agcgcactcc ccgacctgtc ctgcggttat atggttgtgc cccgcttcag
    27481 aggccagcag ctgaaagatc tgaaccagct cgacgcctgg gcaaatgacg gaagcaagga
    27541 ctggatcgcc cgcctttgcg gacttcaggg ctgacttttc cggtcacgca ccttgcggtt
    27601 gtccagaatt tttcctacaa ggagacgctg aaaactgaaa cagctccacc ggagactatg
    27661 ctacctgcct ttatgaatcc atctttcaaa gaaaaatacg cccgagtgcc cgcctcgcct
    27721 ctgctgtggt ttcttcttct ctgcgccttc catttttcct ttaatatatg gtggctccat
    27781 gccgaccgcc acgccatcag cacagacgaa gaaactcata tgattatggc gcgggactac
    27841 tacaccgcac tgtttcttcc gcccggcaat gaaagtatca gcgcccgttt tgaagcactg
    27901 cgacatatcc gcgctgatgt gggcaatccg gtacacccgc cgctgcttca tcttgccggt
    27961 gcctttctgg caaaaatcct gggctacgga atagaccgca tcgcttttgc caacaccttg
    28021 gcatttcttc tgtcactggc aggcttttat ctgctgatgg gctcgtttct ctcgcaaaaa
    28081 aacacgcttt ttgccgtcac tgtcttcagc atgacgccca tggtttacat ggcatcccgt
    28141 tatttcatga cagactttct ttccatggcg cttgtgatct gggtgcttta cacgctgtgg
    28201 aaatcagacg gctacgcccg tatcggctgg actgtgcttt tcggcatctg caacggactc
    28261 gccctgctga cacggacaac ggcagtactt tattatgcgt tgccctgttt tcttgtcgga
    28321 tgcttcggcg tgtaccgtat ttttcaggaa agctccggtt tcgccagtcg ctggaaggag
    28381 ctgatgaaaa tgagtgcccg catggcactg gcggcaggca tcacgctgct tcttgcggca
    28441 ccctggtaca tcatgcacgg ggaacagttc taccagcact ggatgaagcc gcaggctgtc
    28501 cacgaggaaa cgccgctatc tatcctccgg aaaacagcac cgcctccgca gcagacagcc
    28561 gctgcatcga aaacagaagc gacggcatcc gcagcggatg gtcagaaaga aaacagcagc
    28621 ccgaaagggg aaactggaaa agccggatgg cgcctgcgtc tgcacagacg tatcccctgg
    28681 acacgctacc cggtttttgt catcaacaac gctgtttttt tgcccatgtt tcttttaagc
    28741 ctagccggca tgatctgtgc ctgctgcagc ggtgctttca taaaacgaaa agaaatctgg
    28801 ctgctcctga gctggctgtg gggttcctat gttctcctca cactgctgct cagtttttcc
    28861 acaccgcgtt acgctttgca ggcactgccc gcccttgcgt ttttttccgc cctccccttc
    28921 ttcctgattc agggaggcaa acttcggcgg acactgcagg gggtatatct ggcactgctt
    28981 ctctttcaat ttgtcaccct gtctgttcac gccttttcgc cgcttcccga actcaaactg
    29041 ccccttcagc cggatccgga atttcaggct gtctatgatg atccgggtct gtacatctat
    29101 aaatccgtgc tccatgcgtc aagcgcctac ggccgcatgc aggcacctat gcaggacaat
    29161 ttcaaagacc ggatttttga agccatgctg cagaacgagc agaaacaacc ctatttcggg
    29221 gtggaagctc cttatgcccg cctcaacatg agaggcatgc tctttgagga gcagcattac
    29281 tggcttgaca gctcccgccc caatcctttc cggcgtaccg acattccggc tgatctggaa
    29341 ccctatcgca acttccgaca ttacggctgg ggaagagaca tggaggaaat acaacccata
    29401 cttcccatgg tggactatgt tgtctacacg acggaagata tcagcgaggc aaaggagcag
    29461 gaatggcttc acattctcga cagccacgac tttgatctca ttgaacgctt tttcgaagcg
    29521 cgtttcggac aggttggcgc acgttattat gcagtggcgg cacgccggga aatccgtccc
    29581 ctgccggaaa ttaaaacaca ggcagacatt gccgctctgc ctctggaagc tctgtaccgt
    29641 ttccgccatt cgggcaagtg gcggctcctt tcgccccctc ttcagaatat gctggagcag
    29701 cgcatacaag cactctttga agcccgtgga aaaggcgtta ctctcaatga caccgtcacg
    29761 tttcttgggg ccactgcatc ccgggcatct gaggatcgct acatcctgaa cctgatgttt
    29821 aaatcaaatg atccgcctcc gctcagctgc cggctgctgc tgcagggcgt cgtgtcgcct
    29881 gaggatatgg cagttcattt tggaacgccg ggtgaaaagt acggtcaatt ccggtggtct
    29941 ttcgaaccca gcccgtcact tgtcttctgg cccgaacagg ggcatgtgct ggtacgcttc
    30001 cccttttccg ccaggcccat accttacgag ctgagccttg ccttttttct gccaggaaaa
    30061 ggactgtggg atcgggcggt caatctcgga tcagtggact tcagtaaagt acccgatgaa
    30121 aatgcaaccc ggtaattgcg aaaggcaccc tgcatgacag tatctgcccg tcgacagcct
    30181 gcctccgaca acgatccgga ttttcgccgc cttgttcgcg gtgcatttct gtcagcgatc
    30241 agcattctga tttccatggc ggctttgctg attacaggca aactgttcac caatgccctc
    30301 gatcaggaac aggtgggcat cttcgcactg ctgctggtct gttcggattt catcatttat
    30361 gccgccgggc tgggctttgc cgcatccatg cccaaactga tagcggcagc cggacaggaa
    30421 gaccggaaaa agatcattgc ctccgcactt gccggtcaac tg
//
LOCUS       DAOPWB010000017        30296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1442_length_30296_cov_5.879402, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000017 DAOPWB010000000
VERSION     DAOPWB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 30296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30296
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1442_length_30296_cov_5.879402"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..543
                     /locus_tag="PKN92_03010"
     CDS             328..543
                     /locus_tag="PKN92_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47794.1"
                     /translation="MKVFRSIEDVDRANLPEGLSEAVRGTLTTLINAYAESGESYNPE
                     VDGHTMLIEESDTDETSRMNMLHCKLL"
     gene            668..1192
                     /locus_tag="PKN92_03015"
     CDS             668..1192
                     /locus_tag="PKN92_03015"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HNZ47795.1"
                     /translation="MTKSVPTPDYPAFFSALKERILLAHVSAARAVNHELVLLYWDIG
                     QGIVEKQQTAGWGDGVVERLAADLRSAFPDMRGFSSANLWRMKQFYLTHTSAEFLAHV
                     AQEMEKPKTSFLAQPVRETTVTGTASEQLAQAVRELVSAVPWGHHINALSRLTHPAAL
                     RHSCLRRNDDKRRE"
     gene            1431..2792
                     /gene="radA"
                     /locus_tag="PKN92_03020"
     CDS             1431..2792
                     /gene="radA"
                     /locus_tag="PKN92_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNZ47796.1"
                     /translation="MIRLKSRFVCEECGTVHPKWTGKCSACESWNTLQEEEVAEKGVH
                     VRASLGIHSEPVSITEGSEETAPRLSSGMAEFDRVLGGGIIPGSLTLVGGDPGIGKST
                     LMLQLSHHLAAHTGRVLYVSGEESCEQARLRARRLNTLHDRLMLLTETHIAAISPHLS
                     EGHYALAVIDSIQSVYSSQLPAAPGSTGQLRECANEFMRLAKTSNIPVFLVGHVTKDG
                     VIAGPRLLEHLVDTVLYFEGEGRQSLRVLRAVKNRFGSTNEIGVFEMQEGGLREVKNP
                     SALFLEERPVGVSGSVVYPAMEGARPLLVEVQALVSDPHAGSPRRTITGVNPNRVALL
                     LAVLERHGGLRFCDRDVFVNVAGGIRVEEPGVDLAVAAALASSLLNRPVPASLAVFGE
                     VGLSGEVRAVSASRQRAAEVAKFGFTRCLVPKSCSRDIAASGITPEPALTIVEALKVA
                     LEK"
     gene            2794..3882
                     /gene="disA"
                     /locus_tag="PKN92_03025"
     CDS             2794..3882
                     /gene="disA"
                     /locus_tag="PKN92_03025"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HNZ47797.1"
                     /translation="MVKRKTLDEHESFRAALRMIAPGTQIREAISAILQARTGALLCF
                     GATNRLSRLSEGGVKLDEEMTAQLLYELSKMDGAILINETGTRISYANRFLKPSSNYP
                     TSETGTRHRAAQRFANQAKCLVLAISQRRSCVTLYCHSKRYVLDSVPTLINKGTQTLQ
                     ILDKYVDTLHKTLDDLTSRELGNVVTIYDVCRVLQRAEMVHRIAKEVTPIIEELGTEG
                     RLLDIQMQETLKPLNEVALILKDYHREKPGVTVSVIMERMAALPQEELMNLGTISQIL
                     GYGPSRNVDVIMSPRGYHVLHAINRLPAQIIDNLVNELGSLQAILQAPKEVLVEVEGV
                     GDVMAERIRSGLALLANQFGPGSGDTQS"
     gene            3879..4685
                     /locus_tag="PKN92_03030"
     CDS             3879..4685
                     /locus_tag="PKN92_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNZ47798.1"
                     /translation="MIHLLDCKVLSNDEVAPGHRRLVLDGKAIAEDTCAGQFCMLESS
                     PGLQPFLRRPMSIERFGGDSFSVLYKIVGEGTRLLGRIQPGDTVNVQGPLGNGFSIPK
                     EFKRYILVGGGIGIAPLPGLAQALIRETGTPPEVILAARSRDILLCQEDFTRMNCPVF
                     LVTDDGSAGMHGFASNALEARKPDKNTLVYTCGPMIMMGAVHRICMENGSRCQASLEA
                     NMACGDGVCMGCVVETTLQEEFRRMVRVCHEGPVFDSRDIRWNTGTPAHD"
     gene            4678..5613
                     /locus_tag="PKN92_03035"
     CDS             4678..5613
                     /locus_tag="PKN92_03035"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNZ47799.1"
                     /translation="MTEPSLHINLGGLAMKNPVTVASGTFGYGSEYGHYFDVSLLGAV
                     TVKSLSLKPRPGNRPPRISETPAGMLNAIGLQNVGIDVYLKEKLPYLREKNCTIIANI
                     YGYSPEEYAELAQRLEKEGGADALEANLSCPNVHDAKNTCGPALVAQSPQRVKEYTET
                     IRAATSLPLYIKLSPNVADIREPALAAQEGGADAVALINTLLGMNIDVETRKPCLSNI
                     VGGLSGPAIRPVAVKMVWDAARVLNIPVLGMGGIYTADDAIQFLLAGATAVAVGCASF
                     RRPDAALQVIRGMRDYCIRHGIEDIQKLTGALRLQ"
     gene            5775..7055
                     /locus_tag="PKN92_03040"
     CDS             5775..7055
                     /locus_tag="PKN92_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47800.1"
                     /translation="MTEKKRLSRRTFLAAAAAGASVITLPQILPSGVLARNGQPGAND
                     RIGVGVIGPGRQGSDLMSQYPRKGAQLVAFADCYQERLKRAQKRFRKTALYQDYRELL
                     EDSNVDAVIIATPDHWHALNTIHACEAGKDVYVEKPMNLTIREGQLMVAAAQKYGRVV
                     TTGSMQRSMEACRIGCELVRNGRAGKIHTVHTHNYPSPWECDLPEQPVPEGMNWDMWC
                     GQTEPRPYHELLYAPRGNGKTDSRGPLGWISYRPYSGGEVTGWGPHGLDLIQWALGMD
                     GTCPVELWPEGEGGWAPVSFRYANGITVHLDGKGPAGGGVFVGDAGKILVDRNKYDVR
                     PKSLAQEPIGESEKHLEVSTDHMQNWLDCIRSRQKPITTVEVGHSTTTLCHLINITRW
                     VGKKLRWDPETERFDDEEANSHIARTMRAPYNLE"
     gene            complement(7073..8449)
                     /gene="hemN"
                     /locus_tag="PKN92_03045"
     CDS             complement(7073..8449)
                     /gene="hemN"
                     /locus_tag="PKN92_03045"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNZ47801.1"
                     /translation="MHTPEITAEMLVRYDRPGPRYTSYPTVPEWLETFFAVDFRRALY
                     RASKETSQPLSLYCHIPFCERRCAYCGCNTIVGAGDDAMDRYIDHIRAEMSLAAEYLK
                     DRCEVTQCHWGGGTPTRLSLRQMTALFAGITSLYSIHRDAEIALETNPNTVTEEQIRH
                     LRALGFNRISFGVQDLAPEVQRAIGRNQDAEGIRKMTDFCRELGFSGINMDLIYGLPL
                     QQMAAWEKTMEGILAMRPDRVALYSYAHLPQRFEHQRILDKLPRPDTAEKYALFAFAR
                     RVLLEAGYRAIGMDHFAVPEDELALSLEAGTLNRNFMGYTVSQAPDQIGFGLSAISEI
                     GGCYSQNQKAMPLYEQALRKGEFPVDRGMTLSSDDLIRRWTIRRLMCSFVLDHKEASE
                     LFQVDSLAYFEEEHKLLKAYEDEGMILLGAYRWQVTPLGMPFIRNICMIFDAYLGTEG
                     KTLFSRTI"
     gene            complement(8480..9577)
                     /locus_tag="PKN92_03050"
     CDS             complement(8480..9577)
                     /locus_tag="PKN92_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47802.1"
                     /translation="MTLEKDAVLKNSDNRLLEQAAQVLEERRKLGLEGLTGDLAFIMI
                     QTEADRHEAAAAELLRYTGHDLVDAFVCGEEKTFVLRHPASADILVRTGRPGPNPFAE
                     INSHPKSAHLPNTRLETLVFHCRDIEAYRDAQLERGVQFLTDEIQEWGPCRFLQSRPS
                     PYTGNSVGVVEFLGDTGRYRGDACADLPSSLSKPETPWAKAVSTLDHIATRVRAEHRD
                     PAILEYMGLTNYSFAFAIYVPELNSITNVARCGTARFAQVFTSGISVSGSGEGEGPTE
                     AFVRNYGARVHHMAFLTEQIGEMDPALRRDGLEFLSDLVGSEEDGIRQSFSAPSPNTL
                     LVNEYIQRFGDFDGFFTQKNVTLLTEASGRQ"
     gene            complement(9581..10096)
                     /locus_tag="PKN92_03055"
     CDS             complement(9581..10096)
                     /locus_tag="PKN92_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ47803.1"
                     /translation="MILGVMSDTHGNTPLMLQAADRLCSEHGAEHLIHLGDNWEDQEW
                     LYMSGYPVSGVPGLWCPEYRSRRIPKSRVDTFAGMQIAYAHDMQDLPPLTPSLELLLS
                     GHTHRACIGRIEECSYMNPGHLKRLQDRGQPASYGLIVIEPQVMHLSLHYLDGKCFEQ
                     KSVNRNVDTKE"
     gene            complement(10199..11062)
                     /locus_tag="PKN92_03060"
     CDS             complement(10199..11062)
                     /locus_tag="PKN92_03060"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ47804.1"
                     /translation="MSTFQVLPALPPFWKGVIFVLLFCLLVYMLLLFGFVLLENWLLY
                     SRRSQEMGETPADRGWQYEELFLATSGGKSCGWWIPLEGARGAVLFSHGSGRNISHYL
                     RDAALYRACGFSVMLYDYGGYGKSEGKPSERRCYEDGEAFYRYLIEQIGLYPEKIVLA
                     GASLGSGIAVKLASEQKPGALILEAAFTSFPDALQEGHRAGKFFLPRLLARNRFPSRQ
                     RLQSVRCPVLIVHSREDDTIPFAQGEHLYAAAQEPKVFLEIRGSHGGGKFSSGESYSR
                     PLCTFLETHVQ"
     gene            complement(11162..12640)
                     /locus_tag="PKN92_03065"
     CDS             complement(11162..12640)
                     /locus_tag="PKN92_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47805.1"
                     /translation="MADVKKTAGEKNGNGAINRRSFIKTAGAGALIGLAAKQAHASVY
                     KSILPATVLGANERLLTGHIGIGGMGRANLGFALQRDDIQPIAICDIYKKNLFRASQM
                     LKQKYPDFQQYKDFRELLDNKDVDAVVIATSDHWHCLCTLHAADAGKHIYCEKPLSTT
                     IAEGHAMVDAVKRNKVVFQGGNMQRSGTHFQEAVKLIQEGYIGDVAHVETWSHDQDKV
                     ESIGMGETDLAKYESDDFGIDWDFHQGWVEHRPFNTNRWIYNFRWFLEYSGGKITDWG
                     AHLIDIVLWAMGEEKQPRSISSQGGKYIVQDNRTTPDTLEVAWRFDDYVLTFKNRVWN
                     PQLLDGSYSDHGIVFYGTKGMLEVSRGGYEVKSFPANGGCEPVKAGPSDLNQPHWTNW
                     IECIKSGGLPISNIDVLHNTTRVCHIGTCSYVAGTYFNSGAAFENGELPDEAPAYGGS
                     HLEWDDATQRFTKGDPRAVEIANAWAYREYKNGWSLKAPYHS"
     gene            12881..13315
                     /locus_tag="PKN92_03070"
     CDS             12881..13315
                     /locus_tag="PKN92_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47806.1"
                     /translation="MTPQERKALCLEFLKGKGFETSLDEDGDIKFLFEDHEFYLDLDE
                     EDELFFHLIYPAFWSIDNDDEWAEVCEAALQASASTKVAKIIVVDDQVHAAAEMFCPT
                     HEVFEAIFDRAFGTIHAAVNLFLDCIEGVEEQSDEAEEGEEN"
     gene            13455..13832
                     /locus_tag="PKN92_03075"
     CDS             13455..13832
                     /locus_tag="PKN92_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HNZ47807.1"
                     /translation="MASKLLLLALAGAAGTVTRFGISAAVNHFFPSPFPPGTLIVNTA
                     GCFLAGFFWMLFEMRWMHAAESRTILFVGFMGAFTTFSAVMMENSELLRSAAWMPAAL
                     HLSLHNLLGIVAVFAGAALGRLV"
     gene            14002..14763
                     /locus_tag="PKN92_03080"
     CDS             14002..14763
                     /locus_tag="PKN92_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47808.1"
                     /translation="MMSRLHRIVLILIVLPAVFFLNGCIKGTSTVQLDEVEIFSAAAL
                     NLAAAQGRIHLQGDPKRNEEGSARIRTSLQVETYSLFGMAKPDTYLPLVRISPRLEED
                     TLVLATELEARSLWDRLLVRVVPRVDRYLDIPTNLRTSVDLLVGDVEAANLPGDLRIR
                     VDAGHILVAPESEIVGEQRFSIRLGDLEMALPTYTAFRYDLQADLGTISTRNVALSLQ
                     TRFLGARASGVAGLPVRQGTVDAKVRCGSIRVNAE"
     gene            14824..15528
                     /locus_tag="PKN92_03085"
     CDS             14824..15528
                     /locus_tag="PKN92_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ47809.1"
                     /translation="MKRVTAPLEQRLIWLQGCPLFAEVAPEHLKDLAGLMSIRRYEPG
                     ETVFQEGDTADGLYIIVDGQVKIGRYAPDGREQVLHIFEEGEPLGEAAMFEGGHFPAT
                     AQTLTAATILFLLRADFLYLAEKKPALLLNLLAVLSRRLRRFVQMIDDLALKEVSTRL
                     AKHLLELSEEADSSHEVFLTTSKTMLAARLGAVAETLSRTLARMQRRGIIQVDGRRVL
                     LKNREALEALAEGEKL"
     gene            complement(15525..17075)
                     /locus_tag="PKN92_03090"
     CDS             complement(15525..17075)
                     /locus_tag="PKN92_03090"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNZ47810.1"
                     /translation="MAIILSAEEAVKRIPDGATVFVLPMPLEEVFPAFYRVYEETGSP
                     RDLTVVWAAGIGPFNADPRGMNHFAHPGMVKRVILGHVGLNHAMVKVIGANEVEAYNF
                     PQGVISQWFREVAAGRPGLITRVGLGTFVDPRIEGGKLNDRTRQCEDLVEVVTIGDKE
                     LLRYKPIRGDVGVIRGTTADPYGNITSENEAILMEPLEIAMAAKNSGGLVIAQVERLS
                     DTPALPSAVTIPGVMVDIIVVASSQEKHPHTLFADHDPSYTGAARADLSKDLAPMPLC
                     AEKVICRRAFQEISRGDLVNLGVGIPMGVAKVAQEEGLLDNITLTTELGVIGGLPQGG
                     KNFGPAQNPHAIISQAAMFDYYDGGGINISCVGIAQVDGEGNVNVSKLGSHVIGSGGF
                     INITQSSPKVMFCGEFTAAGTDVVVEDGKIVIRSEGKAVKFVKEVGQITFSGKVAREE
                     GHKVLYITERCVFQLTPEGVVLTEIAPGVDLEQDILARMEFRPLISPELKEMDPRLFA
                     DAPMSSSV"
     gene            complement(17269..19038)
                     /locus_tag="PKN92_03095"
     CDS             complement(17269..19038)
                     /locus_tag="PKN92_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ47811.1"
                     /translation="MISRRTILALSIISALVLQVLFLWMAPLITMLESDRLVDRIPTR
                     FQVAYLDQPPPAPRKFVPGQQRALPPGKIESVVGEEPGLLPLEDSAAAPPVETPKLTE
                     RVAGNLEERSYDLEAEPERINKMDAKILEIARESARRDIDVVRRLVRPSPEVTLSASD
                     LPALRSPGITPEAISLEPARLGAGLLAQVIPTGDETVGGAGSGDEKPDQESVAFQAAA
                     DTVEVLPALEKKIAEAPVLREREQLREETPFVLLDDLVEFRLETYVESPAVPGYFRLN
                     IYPKKGSALETLPKDLTIILDGSRSMQHRKLELAARGIADALSFLRPQDRLNIYIFRD
                     TVVPFREQFFPATPENIAAAQQYLRSLEAQGQTDFYNALQMLPGVESRPNQPSIVFVV
                     SDGRPTLGIQDARTIINSTANDNALRKSIFTYGSGNTVDTYLMALLAYRNKGEAFVSP
                     RIQDSQNDVKSWLLRLNDPLLADLRADFGGIMEEESFPRAVPDFFKDKPVVLYGRYTP
                     GEKESFALRITGKAGDKEKEMIFRARFADAATGGREIAQGWAFEKAYYLIGEMSRRGE
                     SAETIGEIRALSRQYAIRTIYDE"
     gene            complement(19041..21413)
                     /locus_tag="PKN92_03100"
     CDS             complement(19041..21413)
                     /locus_tag="PKN92_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47812.1"
                     /translation="MTGIGLPIVIRRSLHAMLLLLMAASGAFAASSGEQMDFANGLFH
                     RGFYKEALDEYKAWLAASPESDDAFAVWLRMGRCALALDNRDEAVHAFDQAAQMAENA
                     AERAEAQASAGEALFFKGDYAAASQRLEKSLADSPAGELRARTLFYLGRAKMERGELL
                     DAASALNSLIKEFPESPLTAFAQYHLAFVHVKLGSAEEAAKAFSAVAGASGADESLRM
                     ESRFRAAELYDSLGWTDTALGAYEMLRRDFPGSSYGLRADYGYAWALYHSGRYEEALQ
                     GASAFLKEHKDSDQAAGMYYLEGNCLQQLHRYEEALKSYQQLGRDYPGTSFAVHGLYK
                     SAWVLYALGNVPAARETAQRYLESGNDGSFIGETRYLLGILHVSEGDFEEALDAFQKV
                     VSEAPDSTFTADALFKMAECYAHLGLRDESARHFEEFARRYPDNPLTEQAILRSGDAR
                     FTAQDFAQAIENYKKLLENPKDPALEEEALYRLAVTCHNAGAYDDSAAAFHRLLDKFG
                     EGRFACEAAFRIAEYELHQRQDPLKALESYEAALRRNPDPDMTFRILQGLASARYEQK
                     DFEKAGELILRLVREYPQSSLPDESYLWSGQWFEKAGRNGEAAEIFQALLKAYPDHKE
                     KAGISFLLAQCLEKEGKNQAAAEHYRLALTAGIGEDREAEVLLRLGALYEKEGDRKSA
                     RDMYDQAANLDSGESAARARFHLAELFEADGQLEEAAKNYMRLAILFVHKTLSPESLW
                     RAGNCYLKMKNTGQAKSIFEELLSDYPDSSFASEAQALLASDAFSAKGSE"
     gene            complement(21410..21838)
                     /locus_tag="PKN92_03105"
     CDS             complement(21410..21838)
                     /locus_tag="PKN92_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNZ47813.1"
                     /translation="MKIRHKFQEETDTIPMAPLIDIVFLTLIFFMVTTVYATLESEVD
                     ILLPTADTAQQGERAQGEIFINLRKDGTIVVNERVMTLDMLQQTLNRVAAYFPGGAVI
                     IRGDSAAALGNAISILDCCRKADIQNVAFAALPAEEKEAP"
     gene            complement(21835..22578)
                     /locus_tag="PKN92_03110"
     CDS             complement(21835..22578)
                     /locus_tag="PKN92_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNZ47814.1"
                     /translation="MFRLLLITCLCLVVLPAAAQAPVSGEVVTADTLPVLRTTDTITL
                     MMMIEQGGVILWIIMGLGFIALVLTLYLLVTVSPRREAPATLVKRAVAQVKAGELQGA
                     YHMCLERDEFLARVFAAGIKMAGHGRFVIQDAMESEGERMAAALWQRITYLNNIAMIA
                     PLLGLLGTVWGMMRAFSAIALDTAQVKGITMAYSVSQAMITTAGGLALAIPAMAVYFL
                     LRGRVLRIIAEVEARSAELIEVLAGENKE"
     gene            complement(22749..23693)
                     /locus_tag="PKN92_03115"
     CDS             complement(22749..23693)
                     /locus_tag="PKN92_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNZ47815.1"
                     /translation="MKRICGFTLIELLVVIAIISILAAILLPALSRAREAARRASCQN
                     NLKQLGFACKLYADESGGNFFPPVKTLHCTGIPISGLSPMMDMERVYPEYLNDFAITL
                     CPSFAFKGDAVALWDQGLNPSSHWHHAEEMGWLPTAGNGVVEPCEVYDHPYIYFGWAL
                     NPSGLDTEDKLHHFEHALLEEPEGLVHRLELDPHLAQSDWMLEERWSDDMAEPVVYRL
                     REGIERFLITDINNAAQGALAQSSLPVLWDALSGESPDHFNHVPGGCNVLYMDGHASF
                     LRYVPTGSDACRNTGSAFPVNGGGIILHEATHGHDHHH"
     gene            complement(23779..24084)
                     /locus_tag="PKN92_03120"
     CDS             complement(23779..24084)
                     /locus_tag="PKN92_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47816.1"
                     /translation="MKKRKLKHLLRRTAFAALAVLWCFVANAAYGFHHHDHGEEECEH
                     SDCPFLQFSFHLPSLPLAASVLPAVTILFPVLLPLAVPRGVCLSVRLSSRAPPALPA"
     gene            complement(24216..26000)
                     /locus_tag="PKN92_03125"
     CDS             complement(24216..26000)
                     /locus_tag="PKN92_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47817.1"
                     /translation="MIRRNFFVIGLLVSLCFVSAQAEYSGGTGKKADPYLIGTVQDWQ
                     QLCATQMHWASHFKVINDLDFFGVTVEPLGWAFEEGSVYYPYQYFSGTLDGQGYSFKN
                     IVLDYPEFSRVALFHELRESTVSNLKIENIVVKGSCSAAALAATLADSTVSGCSITGS
                     ITADSSAAGLAVYCESIILSDCTAGIAVTSEGDDAGGLLCRVGGGRLENCAVEGSVHS
                     KNGLCGGIAASCDYAFLGSCTVDAEIGSFNLDAGGLAGNLREKCAVRNCRTRGKVTAR
                     INGGGLAGFIEGSTVYESFSEAEIIVAGTVKGSGVGGLAGGASETDFYDCYARGSLAG
                     RDLGGGLAGDMRGLRQLPEAQSTVARCYATCDVTVKEGGIAGGLTARAERMVWESSYW
                     DQEKTGIAFSVGGEGRATTDMLYPHAENSYVGWDFDEIWQADNGEVQLNDGYPWLRNT
                     PPFEKKEVVVPEGEEVAEGEELPEGEEVSEGEEILEGEVKPSEGEFNPTEGETAIEGE
                     DATEGEIPPSEGEIPGEGESVLEGEEISEGELEPEGENILPDDSPFGCGCGKKSVQGP
                     VKDLLGDWLLVALSLGVLLSGSVLAKKD"
     gene            complement(26025..27392)
                     /locus_tag="PKN92_03130"
     CDS             complement(26025..27392)
                     /locus_tag="PKN92_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNZ47818.1"
                     /translation="MRVEHDCCTQTLPSVLDHFFGITAPRLKALAERWNPAQGTPVFT
                     RQGKYTSRGWTEWTQGFLYGCRLLCFDAAGDEALLDSGRQDTLRYMAPHLSHVGVHDH
                     GFNNLSTYGNLRRLALEGRSTDSEDLIRLYELALKVSGAVQASRWTKIHGGGGFIHSF
                     NGPQSLFVDTIRSCRILVVAHTLGHRLMGENDEAISLLSRAMDHIRATLQYNVYYGKG
                     RDYYDVRGRVAHESIFNVADGRYRCPSTQQGYSAFSTWTRGLAWGLLGCAEQLEFFRD
                     YPSLCEEQEDGKAFPEVLEEGARAMADFYLENTPSDGVPYWDTGAPGLAEMGDYLNRP
                     AEPDNDWEPVDSSAGAIAAQGLLRLGLYLGEKGEGALYYRAGMQLAQRLFTAPYLSED
                     PAHEGVLLHSVYHRPNGWDYIPDGKKIPQGESSLWGDYHAMELAVCCSRLLKKETPLC
                     FYEKI"
     gene            complement(27413..28840)
                     /locus_tag="PKN92_03135"
     CDS             complement(27413..28840)
                     /locus_tag="PKN92_03135"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ47819.1"
                     /translation="MKYTEAGALEGELASGAFDCILLDFDHTLYLNNSTERFLDSVRP
                     RILAYLLCAFSDWFLQVLAWFRLCSYPVHRDFVRVSLCMLLMPWSLPLWRKKAQHLAG
                     ASMNQWLLERLPADRRTAVISFGFVPLIRPLLDAAGLARVDLIASAPFCFSGNLRRKG
                     KVTALAALLPEEAWEKTLFITDSLDDAELLDALPHGRLIEWGGSSKAAFHGLYLPFRY
                     TVEGKYAGSRYFTYQILLEDLMLLFLAYLFSWQHAGALTALFASLYCIYEIGYYENDH
                     VAVRREEKPVVSDAAAVFPKFPRFRPWLWAFGFAVIGFLFLYGGGDASVQRWGSAALL
                     WSVILLSLRLFFLCFNYLAPGFRVPLFPLLHLYKCFVFACFVPLSLFGALLLTAQVLA
                     ISANYTAYRWIASGLRLNRQLWRLILFLLLSAAVFMWYPMEGQAPSLCWWLLVLIWGG
                     IRSLEHACHKNIVRIVGGLLVPALR"
     gene            complement(28899..29945)
                     /locus_tag="PKN92_03140"
     CDS             complement(28899..29945)
                     /locus_tag="PKN92_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47820.1"
                     /translation="MAKISYGIIGCGNIGPIHGAAIAQVKSAKLVAVSDVVEASAAAL
                     AGQYGAAVYTDYRKLLERDDIQAVCLCVPSGLRMEIAVDCAKAGKHILSEKPLDVTTK
                     RIDRIIAAADAAGVHLGCIFQSRFADDSAKIKKALDEGRFGNLVLGDAYIKWYRSKEY
                     YASGAWRGTKKLDGGGALMNQGIHQIDLLQWFMGPVKTVRAQMTRRLHEGLEVEDTAT
                     AMLEFESGAFGVIEGSTAIWPGHSARIEIHGSSGSVVTEDGAIKFWQFEKRRPADKKI
                     EEALNRESELGSGAADPLSSLKTEGHRRQIDDFTQALLKGRAPLIDGREGRNAVALIE
                     AIYKSAASGKAVHL"
     gene            complement(30096..30185)
                     /locus_tag="PKN92_03145"
     tRNA            complement(30096..30185)
                     /locus_tag="PKN92_03145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30149..30151),aa:Ser,seq:gct)
BASE COUNT         7069 a         8984 c         7757 g         6486 t
ORIGIN      
        1 gtacacgccc aattccggtg cctatactgt gcctaattta cagtaattct atctgcactc
       61 cttcggcgta aagctccgac cgtccacctt aaacttggta attagcggtc tacactttgg
      121 aggaggcgca ccgttccggc atagactgac ctttcgcccc acagtgatgg cagtctctcc
      181 tgcgtcgacg tcccatggtt ccgaatgtcc acccaccggt acgccatctc ccccttttct
      241 atccccacct cctcacccgt attaactccc tttttcaaat ccatattaat cctctgtgga
      301 tagctattta ccctggagga tctaaacatg aaagtatttc gtagtattga agacgtggac
      361 cgggcgaatc tgcctgaggg ccttagtgag gccgtgcgtg gaacgctgac aaccctgatc
      421 aatgcctacg ctgagagcgg tgagtcctac aaccccgaag tagacggaca cacgatgctc
      481 atcgaggaga gtgacaccga cgagaccagc cgcatgaata tgctacactg taaattattg
      541 tgatagccct tgacaaattg cactggcata ccagatcgag gctggctttg aggctgaggc
      601 ggagagaata tttaatgaaa tactcggatt aacggttagc gaaaatgaca gagagcgttt
      661 tttattcatg acaaagtctg tgccaacccc ggattatccg gccttttttt ctgctttgaa
      721 agagcgcatt cttcttgcgc atgtatctgc tgcacgagcg gtcaaccatg aattggtcct
      781 gttgtattgg gacatcgggc aaggtatcgt ggaaaagcag caaactgcgg gctggggcga
      841 tggggtcgtg gaacgccttg ccgctgatct gcgcagtgcc tttccggaca tgcgtggctt
      901 ctcgtcggca aatctatggc gcatgaaaca attctacctt acccacactt cagcggaatt
      961 tcttgcccac gtcgcacagg aaatggaaaa accgaaaacg tcatttctcg cacaacctgt
     1021 gcgagaaacc actgtaacag gaacagcttc ggaacaactt gcacaggctg tgcgagaatt
     1081 ggtttcggcc gttccctggg gacatcacat aaacgcattg tcccgactca ctcatcccgc
     1141 cgccctccgt cattcctgcc ttcgcaggaa tgacgataaa agaagggaat gatgtggtgg
     1201 ataaaatgaa tatttttctg tttccatccg tccttttgca aaagaaagtt ctctggtggc
     1261 aacgtcattc ccctttgtac cctttcttca acagaacagt ttgccggact cgccgtgaat
     1321 acggtacact ttatccaata gaaaacacag ccctctggcc ctgaatccat cgataaaaag
     1381 gacagcatta ttttctggaa gcgcaagaca aacatcaggt ctcttccgct atgatacggc
     1441 tgaaatcacg ctttgtctgc gaagagtgcg gcaccgtcca tcctaaatgg acgggcaaat
     1501 gttctgcctg tgaatcctgg aacacgctgc aggaagaaga ggtggctgaa aagggggttc
     1561 atgtccgtgc ttcgcttgga atacacagcg aacccgtttc cattacagaa ggttcagaag
     1621 aaacggcgcc ccggctgtcc agcggcatgg cagaattcga tcgggtgctc ggaggcggca
     1681 tcatccccgg ttcgctcaca ctcgtcggcg gcgatcccgg cataggcaaa tcaacgctta
     1741 tgctgcagct ttcccatcat ctcgccgccc acacggggcg tgtcctctat gtatccggcg
     1801 aagagtcctg cgagcaggcg cgcctgcgtg caagaaggct gaacacgctt catgaccgtc
     1861 tgatgctgct gacagagacg catattgccg ccatctcccc gcatctcagc gaaggacact
     1921 atgctctggc ggtgatcgat tccatccagt cggtctattc atcccagctt ccggcggcgc
     1981 ccggctccac gggacagctg cgggaatgcg ccaatgaatt catgcgtctc gccaagacca
     2041 gcaacattcc ggtgtttctt gtggggcatg tgaccaaaga cggcgttatc gccgggccgc
     2101 gcctgctgga gcatctggtg gacacggttt tatattttga aggcgaaggc cgtcagtcgc
     2161 tgcgtgtgct ccgtgccgtg aaaaaccgct tcggctccac caacgaaatc ggcgtttttg
     2221 aaatgcagga agggggactg cgtgaagtca aaaaccccag tgcccttttc ctcgaagaac
     2281 ggccggtcgg cgtcagcggt tctgtggtgt atcccgccat ggaaggggcg cgcccgctgc
     2341 tcgtggaagt acaggccctg gtcagtgacc cgcacgccgg ttctccgcgg cgtaccatta
     2401 ccggcgtcaa tccgaaccgt gtcgctttgc tcctggctgt tcttgagcgc catggcgggc
     2461 ttcgtttctg cgaccgtgat gtctttgtca acgtggcggg cggcattcgc gttgaagaac
     2521 cgggtgtcga tcttgccgtg gcggcggcgc tggcgtcgag cctcctcaac cgtcccgtac
     2581 ccgcctctct cgccgttttc ggagaagtag gtctgtcggg cgaggtgcgt gctgtcagcg
     2641 catcccgaca gcgtgccgcc gaggtggcaa agttcggctt cacccgatgc cttgttccca
     2701 aaagctgcag ccgcgacatt gccgcttcag gtatcacgcc ggaaccggca ctcactatcg
     2761 tggaagcctt gaaagtagct ctggagaaat agcatggtca aacgaaaaac actggatgaa
     2821 catgaaagct ttcgtgccgc cctgcggatg attgccccgg gaacacagat cagagaagcc
     2881 atctcggcga tcctgcaggc gcgcacaggt gcccttttat gtttcggtgc cacgaaccgg
     2941 ctcagccgcc tctcagaagg cggcgtcaaa ctggatgagg aaatgaccgc tcagctgctt
     3001 tacgagctgt cgaaaatgga cggtgccatt ctgatcaatg aaacgggcac ccgcatttcc
     3061 tatgccaacc gctttctgaa gccctcctcc aattacccga ccagtgaaac aggaacacgc
     3121 caccgcgcgg cacagcgctt cgcaaaccag gcgaaatgcc ttgtcctcgc catctcgcag
     3181 cgtcgttcct gcgtaacgct gtactgccat tccaaacgct atgtgctgga ttcggtgcct
     3241 acactcatca ataaaggaac acagaccctt cagattctcg ataagtatgt agataccctt
     3301 cataaaacac tggacgatct gacctccaga gagctgggca atgtggtcac catttacgat
     3361 gtgtgccgcg tgctccagcg ggcggaaatg gtgcaccgca ttgcaaaaga agtcacaccc
     3421 atcattgaag agctgggtac ggaagggcgg cttctggaca tacagatgca ggaaacctta
     3481 aaaccattga atgaagttgc cctgatcctg aaagattatc atcgggaaaa gcccggagtc
     3541 actgtttccg taatcatgga acgtatggcg gcactgcctc aagaggaatt gatgaacctg
     3601 ggaacaatca gccagatcct cggctatggg ccaagcagaa acgtggacgt gattatgagc
     3661 ccccgcggct atcatgtgct gcatgccatc aaccggctgc ccgctcagat catagacaat
     3721 ctggtcaatg agctgggttc acttcaggct attcttcagg cgcccaaaga agtgctggtg
     3781 gaagtggaag gcgtgggcga tgtaatggcg gagcggatcc gaagcggtct tgccctcctc
     3841 gccaatcaat tcggacccgg ctcgggagat acgcagtcgt gatccatctt cttgactgta
     3901 aagtgctatc caacgatgaa gtggctcccg gacaccggcg cctggttctg gacggaaaag
     3961 ccattgccga agacacctgc gccggtcagt tctgcatgct cgaaagcagc cccgggctcc
     4021 agcctttttt gcggcgcccc atgtccatcg agcgtttcgg cggcgactcc ttttccgtcc
     4081 tttacaaaat tgtcggagaa ggcacccggc tcctgggcag aatacagccc ggagataccg
     4141 tcaatgtgca gggacctctg ggcaatggct tctccatacc gaaggaattc aaacgatata
     4201 ttctcgtcgg aggcggtatc ggcatagccc cgctgcccgg gctggcacag gcgctgatcc
     4261 gggaaacagg gacaccccct gaagtaattc tcgccgcgcg cagccgggac atactgctct
     4321 gccaggaaga cttcacccgg atgaactgcc ccgtatttct ggtcacggac gacggatcgg
     4381 cgggaatgca cggcttcgct tccaatgcat tagaggcgcg gaagcccgat aaaaacacac
     4441 tggtgtatac ctgtggcccc atgatcatga tgggtgcggt gcatcggatc tgcatggaaa
     4501 acgggagcag gtgccaggca tcgctggaag cgaatatggc ttgcggcgac ggcgtttgca
     4561 tgggctgcgt tgtggagacc acgctgcagg aagaattccg gcggatggtc cgggtctgcc
     4621 atgaaggacc cgtttttgat tcacgggaca tccgatggaa tacaggaact cctgcccatg
     4681 actgaacctt cccttcatat aaacctcggc gggctcgcca tgaaaaaccc tgtcactgtc
     4741 gcatcgggta cattcggtta cggcagtgaa tacgggcatt attttgatgt atcccttctg
     4801 ggcgctgtta ccgtcaaaag cctgtcactc aagccgagac cgggcaaccg tcctccgcgc
     4861 atctctgaaa caccggcagg catgctcaac gccatcgggc tgcagaatgt gggcattgat
     4921 gtgtatctga aggaaaaact gccctacctg cgtgaaaaga actgcaccat tatcgccaat
     4981 atctatggct actcgcctga agaatatgcg gagcttgcgc agcggctgga aaaagaaggc
     5041 ggcgccgatg cgctggaagc caatctttcc tgtcccaacg tacacgacgc caaaaacacc
     5101 tgcggccctg ctctggtggc acagtctccc caaagagtga aagaatatac cgagaccatc
     5161 cgcgccgcca catcgctgcc gctttatatc aaactgtcac ccaacgttgc ggatatccgg
     5221 gaacccgctc tggcggcaca ggaaggcggt gctgatgccg tcgccctgat caacacgctg
     5281 ctgggcatga acatcgatgt ggaaacccga aagccctgct tgagcaatat tgtgggcggg
     5341 ctgagcggcc ctgccatccg gcctgttgcc gttaaaatgg tctgggatgc ggcaagggtg
     5401 ctcaatattc cggtactggg catgggcggc atctacactg ccgacgatgc catacaattt
     5461 ctgctggcag gtgccacagc cgtagcagtc ggttgtgcgt ccttccgccg tcccgatgct
     5521 gccctgcagg tcatccgggg gatgcgggac tattgcatcc gccacggcat tgaggacata
     5581 cagaaactga ccggtgcctt gcgccttcag taatcctgtc ctcgtactgt tcctttgccg
     5641 cggaaacgtc ccctcgaact gcccctcgct atgaattgcc aatttgcgtg ctctcttttt
     5701 cacggcactc agtttgcatt tccatcaggc gccttatcat aatgacagtg acagtacaca
     5761 ggagaaaaaa catcatgacg gaaaagaaaa gactttcccg gagaacattt ttagcagcgg
     5821 ctgcggcagg cgcatcagtc atcacactgc cacagatcct cccttcggga gtgctggcac
     5881 gcaacggaca acccggcgcc aatgaccgta tcggcgtggg ggtcatcggt ccgggcagac
     5941 agggatccga cctgatgagt cagtacccga ggaaaggggc acagctcgtt gcttttgccg
     6001 actgctatca ggaacggctg aaacgggcac agaaacggtt cagaaaaacg gctctttatc
     6061 aggactaccg ggaactgctg gaagacagca atgtggatgc ggtcatcatt gccacgcccg
     6121 atcactggca tgcactgaac accattcacg cctgtgaagc aggcaaagat gtttatgtgg
     6181 aaaagcctat gaatctcact atccgtgagg ggcagcttat ggtggcagcg gcacaaaaat
     6241 acggacgtgt tgtcactaca ggaagcatgc agcgctccat ggaagcctgc cgcatcggct
     6301 gcgaactggt acgcaacgga cgggcaggta aaatccacac tgtgcacacc cataattatc
     6361 ccagcccctg ggaatgtgat ctgcctgagc agcctgtacc tgaaggcatg aactgggaca
     6421 tgtggtgcgg ccagacagag ccaaggccct atcatgaact tttatatgcc ccccggggca
     6481 acggaaaaac cgactcaaga ggcccgctcg gctggatttc ctacaggccc tattcaggcg
     6541 gcgaagtcac cggctggggc ccgcacgggc tcgatctcat ccaatgggcg ctcggcatgg
     6601 acggcacctg cccggtggaa ctgtggccgg aaggggaagg cggctgggca ccggtttctt
     6661 ttcgctatgc aaacggcatt acggtacatc tggacggcaa aggccctgca ggcggcggtg
     6721 tttttgtcgg tgatgcgggt aaaattcttg tcgaccgcaa caagtatgat gtgcgcccca
     6781 aatcacttgc ccaggagccg atcggtgaat ccgaaaaaca cctggaagtc agcacggatc
     6841 atatgcaaaa ctggctcgac tgcatccgtt cccggcaaaa acccattacc accgtggaag
     6901 tcgggcacag cacaaccacc ctttgccacc tcatcaatat aacccgctgg gtcgggaaaa
     6961 aactgcgctg ggatccggaa acagaacgct ttgatgacga ggaggcaaac agccacatcg
     7021 cccgcaccat gcgtgcgcca tacaatctgg aatagcctgt acgcccgcta tctcagatgg
     7081 ttctggaaaa aagggtcttg ccctcggttc ccagatacgc atcaaaaatc atgcagatat
     7141 tgcggataaa aggcatgccg agcggggtga cctgccaccg gtatgcaccg agcagaatca
     7201 tgccttcatc ctcgtacgcc ttcaacagtt tgtgctcttc ttcaaaatac gccaggctgt
     7261 ccacttgaaa cagctccgat gcctccttat gatcgagcac aaaactgcac ataagcctgc
     7321 ggatcgtcca acgacgaatc agatcatcag aagacagcgt cattccccgg tcaacaggaa
     7381 actccccttt tcgcagtgcc tgctcataca aaggcattgc tttttgattc tggctgtagc
     7441 agccgccgat ctcgctgatg gcagaaagtc cgaaccctat ctgatcagga gcctgactta
     7501 cggtgtaacc cataaaattg cggttcaatg tgcccgcttc caatgaaaga gcgagctcat
     7561 cttcaggcac agcaaagtga tccatgccga tggcgcggta acccgcttcc agcagtacac
     7621 gccgggcaaa cgcaaaaagc gcgtactttt ccgctgtatc aggtcggggc agtttgtcca
     7681 gaatacgctg gtgctcaaag cgctggggca gatgcgcata gctgtaaagc gccacgcggt
     7741 ctgggcgcat ggcaaggatc ccctccattg tcttttccca ggccgccatc tgctggagag
     7801 gcagcccgta gatcagatcc atgttgatac cgctgaaccc gagttctctg cagaagtcag
     7861 tcattttccg gatgccctcc gcatcctgat tgcggccgat ggcacgctgc acttcaggcg
     7921 caagatcctg cacaccgaaa ctgatccgat tgaagcccag cgcgcgcaga tgtctgatct
     7981 gctcctccgt cactgtattg ggattggtct ccagtgcaat ctccgcatcc cgatgaatag
     8041 agtagagcga cgttatcccg gcaaaaagtg ccgtcatctg ccggagactc agccgggtag
     8101 gcgtgccacc gccccaatgg cactgcgtca cttcgcaccg gtctttcaga tattccgccg
     8161 caagagacat ctctgctcgg atatgatcaa tatagcggtc catggcgtca tcgccagcgc
     8221 cgacaatggt attgcatccg cagtaggcac agcgtcgctc gcaaaaaggg atatgacagt
     8281 atagcgagag aggctgtgac gtttcctttg atgcacgata cagcgctctc ctgaaatcga
     8341 cggcgaaaaa ggtttcaagc cattcaggta cagtcggata actggtatac cgcgggccgg
     8401 gacggtcgta gcgcacaagc atttccgctg tgatttcagg tgtatgcata gtcaaaggcg
     8461 ccccactaaa aaaacgctct tattgacgac cactcgcctc agtcaggagc gtaacattct
     8521 tttgtgtaaa aaagccatca aaatcgccga aacgctggat gtattcgttt acaagcagcg
     8581 tattgggtga aggggcgctg aaagactggc ggatgccgtc ttcttcagaa ccgaccagat
     8641 cgcttagaaa ttccagtccg tctcttcgca atgccggatc catctcgccg atctgttccg
     8701 tcagaaacgc catatggtgt acccgggcac cgtaattccg gacaaatgcc tcggtcggcc
     8761 cttctccctc gccgctgccg cttacactga tgccggaggt gaacacctgt gcaaaacggg
     8821 cagtgccgca gcgggcaaca ttggtgatgg aattgagctc gggaacgtaa atcgcaaaag
     8881 caaaggagta attggtcaaa cccatgtact ccagaatggc aggatcgcga tgctccgccc
     8941 ggacacgggt ggctatatga tcaagcgttg acaccgcttt cgcccagggt gtctccggct
     9001 tgctcagcga tgacggcaga tcagcacatg catcgcctct gtaacggcct gtatcgccca
     9061 gaaactcaac aacgcccaca gaattacctg tatagggcga aggacggctc tgcagaaagc
     9121 ggcacggtcc ccattcctga atctcatcgg tcagaaactg tacaccccgc tccaactggg
     9181 catcacgata cgcctcgata tcacggcagt gaaaaaccag cgtttccagg cgtgtgttcg
     9241 gaaggtgtgc ggacttggga tgactgttaa tttcagcaaa agggttcgga cccggtctgc
     9301 ctgtacgaac cagaatatcc gctgaagcag gatgacggag tacaaaagtc ttctcttccc
     9361 cgcacacaaa agcatccacc aggtcatgcc cggtataacg gagcagttcc gccgctgccg
     9421 cctcatgccg atccgcctcc gtttgaatca taataaacgc aaggtcgccc gtcagccctt
     9481 ccagccccag cttccggcgc tcttcaagca cctgtgcggc ctgttcaaga aggcggttat
     9541 ccgaattttt cagcacagca tctttttcca gagtcatggc ttactccttt gtgtcaacgt
     9601 tgcggttaac acttttttgc tcaaaacact tcccatccag ataatgcaga gatagatgca
     9661 ttacctgagg ctcgatgacg atcagcccgt aggaggcagg ctgtcccctg tcctgcaggc
     9721 gttttaaatg ccccggattc atatacgaac attcttcgat cctgccgata catgcccggt
     9781 gggtatgccc ggaaagaagc agttccagcg aaggcgtcag aggcggcaga tcctgcatat
     9841 catgcgcata agcgatctgc attccggcaa aggtatccac acgacttttg ggaatgcgtc
     9901 tgctcctgta ttccggacac cacagaccgg gcacgccgct gacaggatac cccgacatgt
     9961 agagccattc ctggtcctcc cagttgtcgc ccagatgaat cagatgttct gcgccgtgct
    10021 cgctgcacaa tctgtctgcc gcctgaagca tcagtggcgt gtttccgtgc gtatcgctca
    10081 tcacacccaa aatcatgatc aatcaccttg ataatcatct gcacgatcat tttatcacga
    10141 agtggcagca gcagaatcag cggaaagatc cgggctcaga gctgcggtcc ccttcgcttt
    10201 attgtacatg agtttcaaga aaagtacaca atggacgact gtagctttcg ccggacgaaa
    10261 atttccctcc accgtgactc ccccgaattt ccagaaatac tttcggttcc tgcgccgccg
    10321 catacaaatg ctcgccctga gcaaatggaa tggtatcatc ttcacgactg tgcacaatca
    10381 gcaccggaca gcggacagac tgcagacgct gcctgctggg gaaacggttg cgcgccagca
    10441 gacggggcag aaagaattta cctgcccggt gcccttcctg cagcgcatcg ggaaaagacg
    10501 tgaaggccgc ttccagaatc agagcgcccg gtttctgttc agacgcaagt ttcaccgcaa
    10561 tccctgaacc caaagaagca ccggcaagca caatcttttc cggataaaga ccgatctgct
    10621 ctatcagata cctgtaaaac gcctcgccgt cctcataaca ccgtcgttcc gagggctttc
    10681 cctcgctttt cccgtagccc ccgtaatcgt aaagcatcac agaaaatccg catgcccggt
    10741 ataatgccgc atcccgcaaa taatgggaaa tattgcgacc gctgccatgt gaaaaaagaa
    10801 cagcaccccg cgccccctcc agcggaatcc accacccgca gcttttcccg cctgatgtcg
    10861 caagaaaaag ctcctcgtac tgccagcccc tgtcggcagg cgtttctccc atttcctgag
    10921 aacggcggga ataaagaagc caattctcca gcagaacaaa gccgaaaagc agcagcatat
    10981 aaaccaggag gcagaaaaga agaacaaaaa tcaccccttt ccagaaaggc ggtaaggctg
    11041 gcagtacttg gaaggtactc ataacagatc cttcttcggg cgcatcaaaa gaaggacggc
    11101 tccctccgga aaaatccggt gaagccgtcc agagaaagga gaatggaacg agtagacttt
    11161 attatgaatg gtagggtgcc ttgaggctcc agccattttt atactcacga tacgcccagg
    11221 cattggcgat ttccaccgct ctgggatcgc ccttggtgaa acgctgtgtg gcatcatccc
    11281 attcaagatg ggatccgccg taggcaggcg cttcatcagg cagttcgccg ttttcaaagg
    11341 cggcgccgga attaaaataa gtgccggcca cataggaaca ggtgccgata tggcagacac
    11401 gggtcgtgtt gtgcagcaca tcaatattgg aaatgggcag accgccggac ttgatgcatt
    11461 cgatccagtt cgtccagtgg ggctgattga ggtcgctggg acccgcctta acaggctcgc
    11521 agccaccgtt tgccgggaag gatttcactt catagccgcc gcgactcact tcaagcatgc
    11581 ccttggtgcc gtagaagaca atgccgtggt cgctgtaact gccgtccaga agctgcggat
    11641 tccagacacg gttcttgaag gtcaggacat agtcatcaaa acgccacgcc acttccagcg
    11701 tatcgggcgt ggttcggttg tcctgaacaa tgtatttgcc gccctgagac gaaatgctgc
    11761 ggggctgctt ctcttcgccc attgcccaga gcacgatatc gatcaggtgc gcgccccagt
    11821 ccgtgatctt gccgcctgag tattccagga accagcggaa gttatagatc cagcggttgg
    11881 tattgaaggg acggtgctcg acccagccct gatgaaaatc ccagtcaatg ccgaaatcgt
    11941 cactttcgta tttcgccaga tccgtttcac ccataccgat gctttccacc ttgtcctgat
    12001 cgtgcgacca ggtctccacg tgggcgacat caccaatata accttcctga atcagcttca
    12061 cggcttcctg aaaatgggtg ccgctgcgct gcatgttccc gccctgaaac acaactttgt
    12121 tgcgtttcac cgcatcgacc atggcatgcc cttcggcaat ggtggtgctc aggggctttt
    12181 cgcagtaaat atgcttgcct gcatcagcgg cgtgcagcgt gcagaggcaa tgccagtgat
    12241 cactcgtcgc aatcacgaca gcatcaacgt ctttgttatc cagcagttca cggaaatcct
    12301 tgtactgctg gaaatcgggg tatttctgct tcagcatctg ggaggcgcgg aaaagatttt
    12361 tcttgtagat atcgcaaatg gcgatgggct gaatatcgtc gcgctgaagt gcgaaaccga
    12421 ggttggcacg acccatgccg ccgataccga tgtgccccgt cagcaacctt tcattggcgc
    12481 ccagcacagt cgccggaagg atggatttgt atacagaggc atgcgcctgc tttgcagcca
    12541 atccgatcag tgcgccggct ccggcggtct taatgaagga acggcggttg attgcgccgt
    12601 tgccgttttt ttcgcctgca gtctttttta cgtctgccat gagtgtcttt ctccctgtcg
    12661 gtggaggttg taacagtaat agaacattgc tccgggcaca gccgaaaagg catgcctgtc
    12721 agaggaatat ggtacctgat tcaggactct gattcaatca caggtaaaag atggaaaaat
    12781 cagtaatctg acggaagaaa aacaaaactt taaccgggtt ccctcttttc tttatgatag
    12841 gcaggtgcga actaaccttt acctgccgga gactataaca atgacaccgc aagaacgaaa
    12901 agccctttgt ctggaattcc tcaaaggaaa aggctttgag acctctcttg atgaagatgg
    12961 cgatattaaa tttctctttg aagaccatga gttttatctg gatctggatg aggaagacga
    13021 actcttcttc cacctgatct accctgcttt ctggtctatt gacaacgacg acgaatgggc
    13081 ggaagtatgc gaagccgcac ttcaagcctc cgcctcaacg aaggtggcga agattatcgt
    13141 tgtcgatgat caggtgcatg cagccgccga aatgttctgc ccgacgcatg aagtctttga
    13201 agccattttt gatcgtgctt tcggaaccat tcatgccgcc gtaaatctct ttctcgactg
    13261 tatcgaaggc gtggaagagc agagcgatga agcggaagaa ggcgaagaaa actgactcgc
    13321 actgctctgt aacagcggtt ttttcagctt ctccggcata acctccagtg aatcggtgca
    13381 acagcactgc cactgcggca gggtgtattg tgctttttcc agtagaactg cggcacttca
    13441 ggagtttctc tttcatggca tccaaacttc tcctgcttgc ccttgccggt gctgccggaa
    13501 cagtcacccg tttcggcatc tcagcggcag tgaatcactt tttcccgtcc ccttttccgc
    13561 cgggaaccct gatcgtcaat actgccggct gctttcttgc cggatttttc tggatgctct
    13621 tcgaaatgcg ctggatgcat gcagccgaaa gccggaccat cctttttgtc ggatttatgg
    13681 gcgctttcac cactttttcc gcggttatga tggaaaacag cgaactgctg cgctccgccg
    13741 cctggatgcc tgccgctctg cacctctccc tgcacaatct gctcgggatc gttgccgttt
    13801 ttgccggtgc cgcactgggc agactcgtct gaaccgcccc gggattaatc tacatgctgc
    13861 ttgataatct ttgcgaaact cagtctgggc agactcgtct gaaccgcccc gggattaatc
    13921 agggttacag tttgtttggt ataatggtac tgtaatcaag tggcaggtat acttacagga
    13981 aactgaaccg gaaagggtcg catgatgagc cgtctgcatc ggattgttct catactgatc
    14041 gtgctgcctg ccgtcttttt cctgaacggc tgtatcaaag gcaccagtac cgtacaactg
    14101 gacgaagtgg aaattttctc cgctgccgct ttgaatctcg ccgcagcaca ggggcgcatt
    14161 catttacagg gcgacccgaa gcgcaatgaa gaaggcagcg cccgcatcag aacaagcctt
    14221 caggtggaga cctacagcct cttcggcatg gcaaagcccg atacctatct gcccctcgta
    14281 cgaatttcac cgcgccttga agaggatacc ctcgttcttg cgaccgagct ggaagcaaga
    14341 agcctctggg atcggctgct ggttcgtgtc gtcccccggg tagaccgtta tctggatatc
    14401 ccgacgaatc tgcgcacttc cgttgatctt cttgttggag atgtcgaagc cgccaacctt
    14461 ccgggcgatc tgcgtatacg cgtggatgca gggcacattc tggttgcacc ggaaagcgaa
    14521 attgtcggag agcagcgttt cagcatccgc ctgggagatc tggaaatggc gctgccgacc
    14581 tacaccgctt tccgctatga tcttcaagcc gatctgggta ccatcagcac cagaaatgtt
    14641 gcgctcagcc ttcagacccg ttttctggga gcccgcgcat cgggcgtggc agggctgccg
    14701 gtcagacagg ggacagtgga cgcaaaagtc cgctgcggga gcatccgggt caatgcggag
    14761 tagcttgccc tccgccgggg cggcactgac ccgaagggcc tgcatcacaa ggaaagattt
    14821 accatgaaac gtgtcactgc cccattggaa cagcgcctga tctggcttca gggctgcccg
    14881 ctctttgcgg aagtggcgcc ggagcatctg aaagatcttg ccgggctcat gagcatacgg
    14941 cggtatgaac cgggagaaac ggtctttcag gagggcgaca cggcagacgg tctttacatt
    15001 attgtcgacg gtcaggttaa aatcgggcgt tatgcgcccg acggacgtga gcaggtactc
    15061 catatctttg aggaaggcga gcccctgggc gaagccgcca tgtttgaagg cggacatttc
    15121 cccgccacgg cgcaaacact caccgccgct accatccttt ttcttctccg tgcggatttt
    15181 ttgtatctgg cggaaaaaaa accggcgctg ctcctgaatc ttctggctgt actgtcccgc
    15241 cggctccgcc gcttcgtgca gatgatcgat gacctggcac tgaaagaagt ctctacccgg
    15301 ctggcaaaac atctgctgga actgtcggag gaagcggaca gcagccatga agttttcctc
    15361 acaacctcca aaaccatgct cgccgcacgc ctcggtgctg tggcggaaac cctgagccgt
    15421 actctcgcca gaatgcagcg ccgcggcatt attcaggtgg acggcaggcg cgtgctcctg
    15481 aaaaaccgcg aggcccttga agcgctggca gaaggggaaa agctttagac cgagctgctc
    15541 ataggcgcat cggcaaagag ccgcggatcc atttctttca gttccggaga aataagcggc
    15601 cggaactcca tacgggcaag gatatcctgt tccagatcca cgccgggtgc aatctcggtc
    15661 agaaccacac cttccggggt cagctgaaag acgcagcgct ccgtaatgta aaggacttta
    15721 tgaccctctt cccgggcgac ctttccgctg aaggtgatct gacccacttc cttcacaaat
    15781 ttaaccgctt tcccttcaga acggatgaca atcttgccgt cctccacaac cacatccgtt
    15841 ccggcagcag taaattcacc gcagaacatg accttgggag agctctgggt gatgttgatg
    15901 aagccgcccg agccgatcac atgggagccc aatttgctga cgttcacatt gccttctcca
    15961 tccacctggg caatgccgac gcaggagata ttgatgccgc cgccgtcgta ataatcaaac
    16021 atggcggcct gggaaatgat ggcatgaggg ttctgcgccg ggccaaaatt cttcccgccc
    16081 tgaggcagcc cgccgatcac gcccagctcc gtggtaagcg tgatattgtc cagcaggccc
    16141 tcttcctgcg caacttttgc cacacccata ggaatgccca cgcccagatt caccagatca
    16201 ccccggctga tttcctgaaa agcgcggcgg cagatcacct tttccgcaca cagcggcata
    16261 ggcgccagat ctttgctcaa atcggcacgg gcagcgccgg tatagctggg gtcatgatcc
    16321 gcaaacaggg tatgaggatg cttctcctgc gaggatgcca ccacgataat atcgaccatc
    16381 acgcccggaa tcgtgaccgc actgggcagc gccggcgtgt cgctgagccg ctcaacctgc
    16441 gcaatcacaa gtcccccgct gtttttcgcc gccatggcta tctccagggg ctccatcaga
    16501 atggcttcgt tctcggaagt gatattgccg tagggatccg ccgtcgtgcc ccggatcaca
    16561 cccacatcgc cccggatcgg tttgtagcgg agcagctcct tatcaccaat cgtcaccact
    16621 tccaccagat cttcacactg gcgggtacgg tcattcaact tgcccccttc aatgcgcgga
    16681 tcaacaaaag tgcccagccc tacccgggta atcagcccgg gccgtcctgc agccacctcg
    16741 cggaaccatt gagaaatcac gccctgaggg aaattatagg cttccacctc attggcaccg
    16801 attactttta ccatggcatg attcagaccc acatgcccca ggatgacccg cttcaccatg
    16861 cccggatgcg caaaatggtt cataccccgg gggtcggcat tgaagggacc gatgcccgcc
    16921 gcccagacta cagtcagatc acggggtgag cccgtctcct cataaacccg gtaaaaagcc
    16981 ggaaacacct cttcaagagg catgggcagc acaaaaactg ttgcgccgtc agggatccgt
    17041 ttgaccgctt cttccgctga aagaataatc gccatgacaa tcgtactcct tgtactgtga
    17101 atagtccggg aaaggggtgt gctcaagccg ggttatttcc tgcagcaggc aaagtgcgcc
    17161 ggataatccc gccgcctctg agtataaacc agtatgcgcc ttctttccaa gcagcgaagc
    17221 gttttcgcta cgatcactca acccactacg tccgcaaacg ctgccgccct attcatcata
    17281 aatagtacgg atggcatact gtctcgacag ggcacggatc tccccgatcg tttccgccga
    17341 ctcgccgcgc cgtgacattt ccccgataag ataataggct ttttcaaatg cccagccctg
    17401 ggcaatctct ctgccgcccg tcgctgcatc ggcgaaacgg gcacggaaaa tcatctcctt
    17461 ctctttatcg cccgcctttc cggtaatacg aagggcaaaa ctctcttttt cacccggcgt
    17521 ataacgccca tacagtacca cgggcttgtc cttaaaaaaa tcaggcactg cccggggaaa
    17581 actttcctct tccataatac cgccgaagtc tgcccggaga tcggcaagca gcggatcatt
    17641 cagacggagc agccagcttt tcacatcatt ctgactatcc tgtatgcgcg gagacacaaa
    17701 ggcttccccc ttgtttctgt atgccagcag cgccatcaga taggtatcca ccgtgttgcc
    17761 ggagccatag gtaaaaatac tcttgcgcag ggcgttgtca ttggctgtgc tgttgataat
    17821 cgtccgtgca tcctgaatac ccagcgtcgg tcttccgtca cttaccacaa agacaatgct
    17881 gggctggttc ggccgggatt ctacccccgg cagcatctgc agtgcgttgt aaaaatcagt
    17941 ctgaccctgc gcttccagac ttctcagata ctgctgggca gcggcgatat tttccggcgt
    18001 ggcaggaaaa aactgttccc ggaagggcac aaccgtatcc ctgaaaatgt aaatgttgag
    18061 gcgatcctgc gggcggagaa aggaaagcgc atcggcaatg ccccgtgcgg caagttccaa
    18121 ttttctgtgc tgcatgctcc gtgacccgtc gagaatgatc gtcaggtctt tgggcagcgt
    18181 ttccagtgca ctgccttttt tcggataaat gttcaggcgg aaatatccag gaactgcagg
    18241 cgattccaca taggtctcca gccggaattc caccagatca tccagcagaa caaagggagt
    18301 ttcttcacgc agctgctccc gttcccggag aaccggagcc tcggcaatct ttttttccag
    18361 ggcaggcagg acttccaccg tgtctgccgc agcctgaaaa gcgacagact cctgatccgg
    18421 cttttcatca ccgctgcctg cgccgcccac ggtttcatcg ccggtcggaa tcacctgcgc
    18481 cagaagtccc gcacccagcc gcgccggctc cagagaaatt gcttcaggcg tgatgccggg
    18541 actgcgcagc gcaggcagat cggaggcgga aagagtcacc tccgggctcg gccgcacaag
    18601 ccgccgcacc acatctatat cccgccgggc ggattcacgg gcaatctcca gaatcttcgc
    18661 atccattttg ttgatgcgct ccggctccgc ctcaagatcg tagctccgct cttccagatt
    18721 accggccacc cgttccgtca gcttcggcgt ttccaccggc ggcgcagcag cactgtcctc
    18781 cagaggcagc agccccggct cttcaccgac gacagattct attttgcccg ggggcaaagc
    18841 gcgctgctga ccgggcacga attttctcgg tgcaggcggc ggctgatcca gataggccac
    18901 ctgaaagcgt gtcggaatgc gatcaacgag acggtcgctt tccagcatgg tgatcagcgg
    18961 tgccatccat agaaacagca cctgcagcac cagggcactg atgatggaca acgccagtat
    19021 ggttctccgg cttatcatgc tcactctgat ccctttgcag agaaggcatc cgatgccagc
    19081 agcgcctgcg cttcggatgc aaaagatgaa tcgggataat ccgaaagcag ttcctcaaaa
    19141 atacttttcg cctgcccggt gtttttcatt tttaaatagc agtttcccgc ccgccacaaa
    19201 gattccggcg aaagggtctt gtggacaaac agaatcgcaa gacgcatata gttttttgcc
    19261 gcctcttcca gctgcccgtc cgcttcgaaa agctccgcca gatgaaagcg cgcccgggct
    19321 gcactctctc cgctgtccag attagcggcc tgatcataca tgtcacgggc ggacttccgg
    19381 tctccttcct tttcgtacag tgcgccgagg cgcagcagca cttccgcttc ccggtcttcg
    19441 ccgattcccg cagtgagcgc aagcctgtaa tgctccgccg ccgcctggtt tttcccctct
    19501 ttttccaggc actgtgccag cagaaaagaa atgcccgcct tctctttatg atccggataa
    19561 gcctttaaaa gcgcctgaaa tatttcggct gcctctccgt ttcgtcctgc cttttcaaac
    19621 cactgcccgc tccacaaata actttcatcc ggaagcgatg actgcggata ttcacgaacc
    19681 agccgcagaa tcagttcgcc cgctttctca aagtccttct gctcatagcg tgccgacgca
    19741 agcccctgca aaatcctgaa ggtcatgtcc gggtcaggat tgcggcgcaa tgccgcctca
    19801 taagattcca gcgccttcag cgggtcctga cgctggtgca gctcatactc agctatgcga
    19861 aacgccgctt cgcaggcgaa gcggccttca ccgaatttat ccagaagacg gtgaaaagcc
    19921 gccgcactgt catcatacgc ccccgcattg tgacaggtca cagccagccg atacagcgcc
    19981 tcttcctcca gtgccgggtc tttcgggttt tccagcagct ttttgtaatt ctcaatagcc
    20041 tgcgcaaaat cctgtgccgt aaagcgggca tcgcccgagc ggaggatagc ctgctctgtc
    20101 agcggattgt cggggtaccg gcgggcaaac tcttcgaaat ggcgtgccga ttcatcacga
    20161 agccccagat gggcataaca ctccgccatc ttgaacaggg catcggccgt aaaagtgctg
    20221 tcgggcgctt cactgaccac tttttgaaaa gcgtccagcg cttcctcaaa gtccccttcg
    20281 ctcacatgca gaatacccag aagatagcgt gtttccccga taaaagagcc gtcatttccg
    20341 gattcgagat agcgctgtgc cgtctcccgt gctgcaggaa cattcccgag ggcatacaac
    20401 acccacgcac ttttataaag accgtgcacg gcaaacgacg tgcccggata gtccctgccc
    20461 aactgctgat aacttttcag ggcttcttca tagcggtgaa gctgctgcag gcagttgccc
    20521 tccagataat acatgcccgc cgcctgatcg gagtctttgt gttctttcag aaatgccgaa
    20581 gcgccctgca gcgcctcctc ataccgcccg ctgtggtaga gtgcccaggc atacccgtag
    20641 tccgcccgga gcccatagga ggaaccggga aaatcacggc gaagcatttc gtaggcgccc
    20701 agcgcggtat cagtccagcc cagactgtca taaagctccg ccgcccggaa gcggctttcc
    20761 atacgcagcg attcatccgc accggacgca ccggcaacag cggaaaaggc ctttgccgct
    20821 tcttcggcac tgcccagttt cacatgcaca aaagcaagat gatactgcgc aaaggcggtg
    20881 agcgggcttt caggaaattc tttgatcaga ctgttcagtg cggaagccgc atccagaagc
    20941 tcgccccgtt ccatctttgc tctgcccaga taaaaaagtg tgcgcgcccg cagttctccg
    21001 gccggggaat cagccagcga cttttccagc cgctgggacg cagcagcata atcaccttta
    21061 aaaaacagtg cctcacctgc actcgcctgc gcttcagcgc gttcagcggc gttctccgcc
    21121 atctgcgccg cctgatcaaa ggcgtgcacc gcctcgtccc gattgtccag tgccagcgca
    21181 caccgtccca tccggagcca cacggcaaaa gcatcgtcac tttccggcga tgccgcaagc
    21241 caggctttgt attcgtccag tgcttcttta taaaaaccgc gatggaacag cccgttggca
    21301 aagtccatct gctctccgga agaagcggcg aaggctccgg aagccgccat cagaagcaat
    21361 agcatggcat ggagacttcg ccttatcacg ataggcaaac cgatgccggt catggcgcct
    21421 ccttttcttc agcgggcagt gctgcaaacg ccacattctg tatgtccgct ttccggcagc
    21481 agtccaggat ggagatggca ttgcccaatg ccgcggcact gtcgccccgg atgatcacag
    21541 cccccccggg aaaataggcg gcgacacggt tcagcgtctg ctgcagcata tcaagcgtca
    21601 tgacccgctc gttcaccaca atggtgccgt ctttacgcag gttgataaaa atctctccct
    21661 gggcacgctc cccctgctgt gccgtatctg ccgtgggcag aagaatatcc acctcgcttt
    21721 ccagcgtggc gtataccgta gtcaccataa agaaaataag ggtaagaaaa acaatatcga
    21781 tgagcggcgc catgggaatg gtgtcggttt cttcctggaa tttatgacgg atcttcattc
    21841 tttattctcc ccggcaagta cctcgatgag ctctgcggat cgcgcctcca cttccgcaat
    21901 aatccgcagc accctgcccc gcagcagaaa ataaacagcc atggcgggaa ttgcgagtgc
    21961 cagaccgcct gccgtggtga tcatcgcctg ggaaacgctg tatgccatgg tgatgccctt
    22021 gacctgtgcc gtatcgagcg caatggcact gaaggcgcgc atcatgcccc agactgtgcc
    22081 cagcagtccc agcagcggcg caatcatggc gatgttgttg agataggtga tccgctgcca
    22141 gagtgccgcc gccatgcgtt ccccttcact ttccatggca tcctgaatca caaaacggcc
    22201 atggcccgcc atcttgatac cggcggcgaa aacgcgcgcc agaaattcat cccgctcaag
    22261 acacatgtgg tatgcgccct gaagctctcc cgctttcacc tgcgccacag cccgcttcac
    22321 cagcgttgcg ggcgcctcac ggcggggact caccgtcacc agaagataca acgtcagcac
    22381 aagcgcaata aagcccagcc ccataatgat ccacagaatg acaccgccct gctcgatcat
    22441 catcatgagc gtaatggtgt cggtggttcg gaggacggga agggtatccg ccgtgaccac
    22501 ctcgccggat accggcgcct gagcagcagc cggaagcaca accagacaca ggcatgtaat
    22561 taaaagcaaa cgaaacattg aggggttctc ctgctgccgg aacgccggcg taacctgggg
    22621 ctctcatttc tgatttcaag accgcggggg caggcacact tcttttatgg ctgtccctgc
    22681 aaccttcata tgtatatgct cttttttgcg gaaaagttgc aaaaactgct tttttttacg
    22741 cccgctcctt aatgatggtg gtcatgaccg tgcgtcgctt cgtgcagtat gatgccgccg
    22801 ccgttcacag gaaaagcact gcccgtattt cggcaggcat cagaaccggt aggcacataa
    22861 cgcagaaagg atgcatgccc gtccatgtac agcacgttgc agccccccgg aacatgattg
    22921 aaatggtcgg gtgactcgcc ggacagcgca tcccacagga caggcagcga tgattgcgcc
    22981 agagcaccct gagcggcatt gttgatgtcc gtaatcagga atcgttcaat gccctcgcgc
    23041 aaccggtaaa caacaggttc cgccatgtcg tcgctccatc gttcctccag catccagtcg
    23101 ctctgggcaa ggtggggatc cagctccaga cggtgaacca gcccttccgg ttcctccaga
    23161 agcgcatgct caaaatgatg caatttatct tctgtatcaa gaccggacgg attcagtgcc
    23221 cagccgaaat aaatataggg atggtcatag acctcgcagg gctccacgac accgttgccc
    23281 gctgtcggaa gccagcccat ttcctcggca tggtgccagt ggctggaggg attgagtccc
    23341 tgatcccaaa gagcgacagc gtcgccttta aaggcaaaac tcgggcagag ggtaatcgca
    23401 aagtcattaa ggtattccgg ataaacccgc tccatatcca tcatgggcga cagcccgcta
    23461 atgggtattc cggtgcaatg caatgtcttc acgggcggga agaaatttcc gccgctttcg
    23521 tctgcataca atttgcaggc aaacccgagc tgtttcagat tgttctgaca gctggcgcga
    23581 cgtgccgcct cccgtgcccg ggaaagtgcc ggaaggagaa tggcagccag gatactgatg
    23641 atggctatca ccaccagcag ttcgatcaaa gtaaaaccgc agattctttt catgaaaagt
    23701 gctccctgcc ggtgccggaa aagcagcggc atttgcgatg cagatgctgc atctcctgaa
    23761 aatgaaaggg tgcatgtttc aggcagggag cgcaggcggc gctctggagg agaggcgtac
    23821 gctcagacaa acgccccggg gtaccgccag gggcagcagc accggaaaca gaatagttac
    23881 agccggaagc accgacgccg ccagaggcag ggaaggaagg tgaaaactga actggaggaa
    23941 ggggcagtct gaatgctcgc attcttcctc gccgtggtcg tgatgatgaa atccgtaagc
    24001 cgcatttgca acaaagcacc acagcaccgc caacgctgca aaagctgtac gacgcagaag
    24061 atgcttcagt ttacgctttt tcatcctgaa tccctttcca caaacaaagc atacagcagt
    24121 atagcactac tgaaggctgc acatataaaa accggtacag ccgcaaaacg gctgtaccgg
    24181 caaacagcat ttgtcagaga cagttcagga aaaaatcagt cttttttggc aagtacactc
    24241 ccggaaagca gtacacccag actcagtgct acgagcagcc agtcgcccag aaggtctttc
    24301 accgggccct gcacactctt cttaccgcag ccgcatccga aaggcgaatc atcaggaagg
    24361 atattctcgc cttccggctc caattctcct tctgaaattt cttcgccttc caatacggat
    24421 tcgccttctc ccggaatttc tccctccgac ggaggaattt caccttcagt tgcgtcttcg
    24481 ccttcgatgg ctgtttcacc ttcagtagga ttaaattcac cttccgaagg cttcacttcg
    24541 ccttccaata tttcttcccc ttccgacact tcctctccct ccggcaactc ctcaccttct
    24601 gccacttctt ctccttccgg cactacgact tccttctttt cgaagggcgg tgtgttgcgc
    24661 agccagggat aaccgtcatt gagctgcact tcaccattgt ccgcctgcca gatctcgtca
    24721 aaatcccagc cgacataact gttttcagca tggggataca gcatgtccgt cgtggctcgt
    24781 ccctctcctc cgacactgaa agcaatgccg gttttttcct gatcccagta agaagattcc
    24841 cagaccatcc gctccgccct ggccgtaagc ccgcccgcaa tacctccttc cttcacagtg
    24901 acatcacagg tggcatagca gcgcgcaaca gtactctgcg cttcgggcag ctgtcgcaac
    24961 ccgcgcatgt cccccgccag gcctccgccc aaatcccgcc ctgccaaaga accgcgggca
    25021 taacagtcat aaaaatctgt ctcactggca ccgccggcaa gcccgccaac gccacttccc
    25081 ttaacagtgc ctgccacgat aatttccgcc tcggaaaaag attcgtagac cgtgcttccc
    25141 tcgataaatc cggcaagtcc gcccccgttt atccgggctg taacctttcc tctggtacgg
    25201 caattccgaa cagcacactt ttcacgcaaa ttaccggcaa gaccgcccgc atccaagtta
    25261 aagcttccaa tctccgcatc aacggtacag gatccgagaa atgcatagtc acaggacgcc
    25321 gctataccgc cgcataagcc attcttcgaa tggacactcc cttccaccgc acaattttca
    25381 agccgacctc ctcccacgcg gcacaacagc ccccctgcat catcaccctc gcttgtcaca
    25441 gcgatcccgg cagtacagtc ggataaaata atggactcgc agtacaccgc cagaccggca
    25501 gcactgctgt ctgccgtgat actcccggta atactgcatc cgctgacagt gctgtctgcc
    25561 agagtcgcgg caagagccgc cgcagagcaa ctgcctttca ccacgatatt ttcaatctta
    25621 agattgctaa cagtactttc ccgcagctcg tgaaaaagcg caaccctgga gaactccggg
    25681 taatccagca caatattttt aaaagaatac ccctgaccat ccagcgttcc gctgaaatac
    25741 tgatagggat aatacacgct tccttcttcg aaggcccagc cgagaggctc aacggtaacg
    25801 ccgaaaaagt ccagatcatt gatcacttta aaatgtgaag cccaatgcat ttgcgtggca
    25861 caaagctgct gccagtcctg caccgtgcca ataagatagg gatccgcctt cttacctgtg
    25921 ccaccgctgt attctgcttg agcactgaca aaacaaagac tcaccagaag accgataacg
    25981 aaaaaattgc gtctgatcat aacaacactc cttttctcta tcacttatat tttctcataa
    26041 aagcagagtg gcgtctcttt tttgagcagc ctgctgcagc atacggcaag ttccatcgca
    26101 tgataatcac cccagagcga agactctccc tgaggaatct tcttgccgtc aggaatataa
    26161 tcccacccgt tcggacggtg ataaacactg tgaagcagca caccttcgtg cgccggatcc
    26221 tcgctcaggt agggagccgt aaaaagcctt tgtgccagct gcatgcccgc ccgataataa
    26281 agcgctccct cccctttctc gccgagatac agacccagac gcagcagccc ctgcgcggct
    26341 atggcgcctg ccgagctgtc cacaggctcc cagtcattgt ccggctctgc cgggcggttc
    26401 agataatcgc ccatctccgc cagaccgggc gctcctgtat cccaataggg caccccgtca
    26461 ctgggtgtat tttccagata gaaatccgcc atggcacgcg ccccttcttc cagcacctcc
    26521 ggaaaggctt tgccgtcttc ctgctcttca caaagcgaag gataatcccg gaaaaactcc
    26581 agctgttccg cacagcccag cagcccccag gcaagccccc gggtccaggt gctgaaagcg
    26641 gaatagccct gctgcgtgga aggacagcgg taccgtccgt cagcgacatt gaaaatactc
    26701 tcatgagcga ccctgccgcg cacatcataa taatcccggc ctttcccgta atacacattg
    26761 tattgaaggg tggcgcggat atgatccatc gcccggctca gcagggaaat cgcttcatca
    26821 ttttcgccca tcagccgatg ccccagcgta tgtgccacca ccagaatacg gcaggagcgg
    26881 attgtatcca caaaaaggga ctgaggcccg ttaaaagaat gaatgaatcc gccgccgccg
    26941 tgaatcttcg tccagcgcga tgcctgcacc gcacccgata ctttcagcgc cagttcatac
    27001 agccggatca gatcttccga atcagtgctc cgcccttcca gcgccagccg ccgcaggttt
    27061 ccataggtcg acaggttgtt gaagccatga tcatgcacgc ccacatggct caggtgcggt
    27121 gccatgtaac gaagcgtatc ctgccgcccc gaatcgagca gcgcttcatc ccccgcagca
    27181 tcaaagcaca gaagcctgca gccgtataga aagccctgcg tccattcggt ccagccccgg
    27241 ctcgtatatt tcccctgccg tgtgaataca ggcgtaccct gcgccgggtt ccaccgttcc
    27301 gccaatgcct tcagccgcgg tgcggtaatc ccgaaaaaat gatccagcac ggacggaagt
    27361 gtctgagtac aacaatcatg ctcaacccgc acggcatgct cctttcacac attcagcgca
    27421 gcgccggaac cagcagccct ccgacaatgc gcacaatatt tttatgacag gcatgttcca
    27481 gagaacggat gccgccccaa atcagaacca gcagccacca gcagagagac ggtgcctgcc
    27541 cctccatcgg ataccacata aagaccgctg cgctcagcag cagaaaaagg atcagccgcc
    27601 ataactgtct gttcagccgg agcccggaag cgatccatcg gtaggcggta taattggcgg
    27661 aaatagccag aacctgtgcc gtaagcagca gcgccccgaa aagagacagc ggcacaaaac
    27721 aggcaaagac aaaacattta tacaagtgca gcagcggaaa aagcggcaca cgaaacccgg
    27781 gcgccagata attgaaacat aggaaaaaca gacgcagact cagaagaatc accgaccaga
    27841 gtaacgcagc gctgccccag cgctgtaccg atgcatcgcc gcccccataa agaaacagaa
    27901 aaccgataac agcaaagcca aaggcccaca gccagggacg gaaccggggg aacttcggaa
    27961 acacggcagc agcatcagaa acaaccggct tctcttcccg ccgtacagcg acatgatcgt
    28021 tttcataata tccgatctcg taaatacagt agagtgaagc aaaaagagcg gtcagtgcgc
    28081 ctgcatgctg ccacgaaaac aggtatgcca aaaacagaag catcagatct tccagcagga
    28141 tctgataggt aaaataacgg ctcccggcat atttgccttc aacggtataa cggaaaggca
    28201 gatagagacc atgaaatgcg gctttgctgc taccgcccca ctcgatcagt ctgccgtgag
    28261 gcagcgcatc cagcagttcg gcatcgtcca gggaatcggt gatgaaaagc gtcttttccc
    28321 acgcttcctc cggaagaagc gctgctagcg ccgtcacttt accctttcgg cgcagattac
    28381 ctgaaaaaca gaaaggcgcc gatgcgatca ggtcaacccg ggcaagccct gctgcatcca
    28441 gaagaggacg gatcagcggt acgaaaccaa agctgataac tgctgtgcgc cggtctgctg
    28501 gcagccgttc gagaagccac tgattcatgg aagcccccgc gaggtgctgc gcctttttcc
    28561 gccacagcgg gagactccag ggcatcagca gcatacagag acttacccgc acaaagtccc
    28621 tgtgcacagg ataggagcag agacggaacc acgccagcac ctgcaggaac cagtcgctga
    28681 aagcgcagag cagataggcg aggatccgcg ggcgcacgct gtccagaaaa cgttccgtgg
    28741 aattgttcag ataaagcgta tgatcaaaat cgaggaggat acaatcgaag gcacccgaag
    28801 ccagctcccc ttccagtgcc ccggcttcgg tatatttcat ttcctcttcg gtacctttca
    28861 ttgctgcccg ccgccgtatt cctgacaggc ggagcagatc aaagatgcac tgcttttccg
    28921 ctggcggcgg atttataaat cgcttcaatc agggcaacag cgttgcgccc ttctctgccg
    28981 tcaataagcg gcgccctgcc tttcagcagt gcctgtgtga agtcgtcaat ctgcctgcgg
    29041 tgcccttcag ttttgagact gctcagcgga tcagcggcac cggagccgag ttccgattcc
    29101 cggttcagcg cttcttcaat ctttttatcc gcaggacgac gcttctcaaa ctgccagaac
    29161 ttgatggctc cgtcttctgt caccacactg ccgctgctgc cgtggatttc aatccgggcg
    29221 gaatggcccg gccagattgc cgtactgccc tcaatcactc cgaaagcacc gctttcaaat
    29281 tcaagcattg ccgttgccgt atcttccact tcaaggcctt catgaagcct gcgtgtcatt
    29341 tgggcacgca ctgttttcac aggacccatg aaccactgaa gcagatcgat ctgatgaatc
    29401 ccctgattca tgagggcacc gccgccgtcc agttttttcg tgccgcgcca ggcgccgctc
    29461 gcgtaatatt cctttgaacg gtaccatttg atataggcat cgccaagcac caggttgccg
    29521 aagcggcctt catccagtgc ctttttgatt ttcgcggaat catcggcaaa acggctctgg
    29581 aaaatgcagc ccagatgaac ccctgccgca tccgccgccg ctataatacg atcaatgcgt
    29641 ttcgtggtta cgtccagcgg tttttcagaa agaatgtgtt tccccgcttt ggcgcagtcc
    29701 acggctattt ccatgcgcag cccgctcggc acacaaagac agaccgcctg tatatcgtcc
    29761 cgttccagca gcttacggta gtccgtatag acagcggcac cgtactgacc cgcaagcgcc
    29821 gctgctgatg cctcgaccac gtcggacact gccaccagct tcgctgattt cacctgagca
    29881 atggcggcgc cgtgaatcgg tccgatgttg ccgcagccga taatgccata actgattttt
    29941 gccatgtcgt aaaggctcct ttcagggttg ttgctcaaca gatcccttat tctactatag
    30001 catacacagg aatacggaaa aagccccgat caggcacacc gcacccgatc atgaaaagaa
    30061 agaaaaaaag gggggagggg gagaaagaaa aagactggcg gagagggagg gattcgaacc
    30121 ctcggtacgg tttcccgcac aacgccttag cagggcgcca ccttcagcca ctcggtcacc
    30181 tctccgtggc ttcttgtagt gtaacatgag atttgagagc aggtcaaaat tgtgaactca
    30241 atacgcgggt tcaaattcga aacgcaatta ttgagaaaca aaaaaggaga cattgc
//
LOCUS       DAOPWB010000018        28893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1573_length_28893_cov_5.935502, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000018 DAOPWB010000000
VERSION     DAOPWB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 28893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28893
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_28893_cov_5.935502"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1028
                     /locus_tag="PKN92_03150"
     CDS             63..1028
                     /locus_tag="PKN92_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47821.1"
                     /translation="MHIRFEKQTALLLLVIFSVFLCLGGGAYYYFFIAPRYGIYHEDL
                     LAPDTALRVVFKPVLVQKYISSLAPAGSSLIPGIPKVSSMQQMHFRTEWIHKMPFEFS
                     FLLDQRASDQLGVLFFVKEHPASESFSELVDDGGFFRATRPIVWDQNRMSRYAVRTLT
                     ATGSVLIPRTYQEETAKHHPVYSPLTPPEVKGHHFLEAALNNKNGTLIELLGAFSAPL
                     RPILSTDTEKTLISLSPNIEGAFLKAFLSEADLLLIEVTVECSDSSSAEEVSKAVLAA
                     AYDTAAFAKREGFALYWDSDIEDNHVLLTGEFSDFEPKIRRAMGG"
     gene            complement(1143..2177)
                     /locus_tag="PKN92_03155"
     CDS             complement(1143..2177)
                     /locus_tag="PKN92_03155"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ47822.1"
                     /translation="MKVSAFYRNKAVLTLIAGGLVLMLLVCPVLCARETKGADDFYPQ
                     ERDPYAGNWTGRWSDDEDVDPDISAQVTATGRNKYTLRLASRLFMRCPVLAVMESRVK
                     NGVLEFSDANYRVKIENGVLTGGRSSGSSTFSMKPFIYENPGLGTPPPEGADVLFDGA
                     GFDAFTGSEGWQIVGDAMMVMPGTEDLVSKNKYLDAQLHVEFRTPLQPTEKGQSRGNS
                     GVFLQDTYEVQVLDSFGLEGTYDECGALYKLSAPHVNACLPPGEWQSYDIRYTAARFS
                     DDGTLLANPRITVYHNGILIHNYQELGWITGWKEKDRLQPPPREAGSVKLQSHRNHYV
                     QFRNVWIKAL"
     gene            complement(2207..4711)
                     /locus_tag="PKN92_03160"
     CDS             complement(2207..4711)
                     /locus_tag="PKN92_03160"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNZ47823.1"
                     /translation="MRTPILLVVLTALFCIGLLGIVGYTQAQSEPLEGYTLALNGAPN
                     QRYTVGDVYFEAGLSIHPPLDDAEKKVKVSLFILPSLNAAHTTYLAHIGSEFNEKGFN
                     QKAAKYELRYSLGSSSVVRQFCYASLAGARIALLGDAEMTVECGTSFIDPGAAADDEC
                     DGDLTSQIIAAGNVNTKSPGEYTRLYSVTNSQGNKTEVTRKITVVDTLKPALQLLGRE
                     IINIPLNGTFTDPGATASDSCDGDLTGSILVDDSELNPSEAGDYSVRYSVQDSAGNVA
                     EAVRTVRVGCVSPGPVIELNGDAVMEAECGEIFADPGVQAWDECEEDEINDTVEYTDD
                     GSFASTGTVRRTYKVTNYMGLQAQVTRTITVVDTKAPVITLNGKETVSLLVGDNYTES
                     GADAFDACEGAVSVSVEGVDAVDTSVIGRYFVTYRASDSLGNEAVKTRLIRVGCGTPE
                     FKLLGSATMVVECGDPYQEPGFTASDSCVDDLRDEVVVHYLQGSVNTQVPGEYTIRYQ
                     LYDWTKVRTVHVIDSKGPVLTLEGAEAMDLHVGTPFVEPGYTAVDACIGDLSSAVRVR
                     GTVNENEEGTYILIYTVQDIYGNESKTERTVTVTFNPEKPIVKLIGAAIVTVECGDQF
                     TDPGATATNEIGTVEYPVTATKLPKEALSVPGEYVIIYSATNELGNTLDVARKVIVVD
                     TKKPVIVLRGAAEVIVDEGSVYVDPGVLSALDDCDGDLRKDVLFESDVDTSAVGVYHV
                     EFFVRDSSGNRAMAVRTAQVRKAVAPGEGENEGEVVEGEPDTEGEDPVTEGEDVDPDD
                     ECPGCGCRHGGIIGDLFMAGLSLAFIFFMIFIHEKI"
     gene            complement(4918..7161)
                     /locus_tag="PKN92_03165"
     CDS             complement(4918..7161)
                     /locus_tag="PKN92_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47824.1"
                     /translation="MHALFLALAVYTAAVLPAETGPEALAFTHFPDRLHAFVWRNWNL
                     VPLERMAAAVGTDASQILETGRSMGLPHPPDISKEQSARSYITIIRANWHLLPFDQLL
                     TLLEWDAEKLAYTLREDDFLLIKLGGFKPVCAPLQYHAPDASVKLRVEEIKDLVHQYF
                     GEEAGSWKTPPFSFIKDLCEPVTHSEPVEAINRFNPRFCYSYFALYGDPFLEQDPFPD
                     GYLQRLAESGVNGVWLHAVLYKLSPFPWAPELSEGWEQRLESLRAMTERAARFGIGIY
                     LYFNEPRAMPLAFFEKYPDLKGVEENGYATLCTARPEVASYITDATASIASAAPALAG
                     ILTITASENLTNCWSHHRGDMCSRCAERGAAKTIADVNTFIASGLDRAGSNARLLAWD
                     WGWAEPWAQECVALLPRRASLMSVSEWDAPVSVGGVSTKVGEYSLSVVGPGPRALRQW
                     EAAKNAGLGAVAKIQANTTWELGALPYIPAVANVAEHITHLRESGVSGLMLGWTLGGY
                     PSPNLEVVYALGEPLADGRFRTADEALHSVAEKRFGSDAAGTMVHAWKKLSDAFKEFP
                     YHGGVVYNAPLQVGPANLLWREPTGYKATMVGLPYDDLDAWRVVYPAGTFLDQLEKVA
                     SGFRESALLMESALEKGNVHATAVQQEMHLAQAAALHYQSIAQQGRFILQRNELAKEA
                     NKEKQQKIREEMKELLRQELECARSLYELQRYDSRIGFEATNHYFYTPADLVEKVLNC
                     ARLMESL"
     gene            7317..9362
                     /locus_tag="PKN92_03170"
     CDS             7317..9362
                     /locus_tag="PKN92_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ47825.1"
                     /translation="MKKLYIHAIVLLAAGSYLALFSAYAQPSAFYPEQITPAEVTKPA
                     PSDQTSSSSGTAEPAEDPVEAFIRNRSLQQRIAQLMIVTLEGESRLLLGDVTYLKSFS
                     PGGILLARSLNPSTALSYVQSVRSFEAVSGAPFLIGADLFALTQPTRSLPSQFIQLPT
                     PLSVLATSSPDIFQDYSALLALYTESLGFNFYPGPALDLSPPFKTAADTVLTLGSDPV
                     KAAGIASCFQNAFAGTRTAWMPMGFPGGGANREGRSAAVLTTPLSALLEQDGLPYRQV
                     IEQGAGIIHVGNVLVPTLDTSSPPASLSKQVMTDLLRHQLNFQGVIAAGPLDEALIQA
                     ETDPAVSAVMALLAGADMLLWQSNLVQPEKANKRIAAAVTDGTLSEERINDSVRRVLR
                     LKMNLAQATPAQLTSKNKRVDLKKLGEWSVKVERHAATLIKNQGNALPLVKDGGAPVG
                     ITGVVGVEELYQLLEKEVKRLVRQSITSARHVGDIERFEIDRLTRNMKALGTIVVILT
                     DRVRFESQIELLRALKQNGAKLVVLFLGDPRNACRLQEADSILLSYCNSNATLQAMQA
                     LAPVLMGKSPLRFRDFPDEIRVKAGEERSFDSKEILCSPVGRLPIALSDQYPAGFSLS
                     MDPGPAIKKVEWFFDGKRIKKDKVAFTFSTPGKHTVSVTVTDMFKTEQSREIPFYAE"
     gene            complement(9371..10864)
                     /locus_tag="PKN92_03175"
     CDS             complement(9371..10864)
                     /locus_tag="PKN92_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNZ47826.1"
                     /translation="MFVCVLALSVCASASPFYIVEVTAPDEVALADLLNCGLTIDNRV
                     DRKVTFYVNEEELEQLKKLSYPLHLLETIPDGSSAEKASGYTSYDQIGPLFSSYANQY
                     PNIVQTGTLGQTPQYREIWAIKITLTPEQEADKPVVKYIAAIHGDEIVGVEMCLYFIQ
                     DLLENFGKNPFISDLICKTVIWVVPIMNPDGHAAVSRYSAGKDLNRNFPAYGQHYSTT
                     LFDGEPLGDEKVPVEDGCIMRWHVRYPANLCANFHGGALLANYPYDNTPGISSGSPAI
                     SPDDDVFRYLALEYSRNNPPMYQSPIFPQGIINGSAWYSITGGMMDWHYRFLGCPELT
                     LEISADKRPPASQLPRFWDENRDAMYAYLSAVHMGIRGLVKDRTTKLPLWAKVMTHDR
                     AQPVFTSRAVGNYHRLLLPGTYNLTYQAPGYITYHADAIAVESGGATRKDIFLSDGDV
                     NQDGTKDAEDVQAVVNAALGKATSFDADVDGRGLSATDIQAVINAVL"
     gene            complement(10906..11460)
                     /locus_tag="PKN92_03180"
     CDS             complement(10906..11460)
                     /locus_tag="PKN92_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47827.1"
                     /translation="MEIFSGNTMLSRLKATACVSGLVLCLFLSGCQTGGGSVADKVLA
                     DFGLKEHPEGHVSRTDMAFEQLDLVGTTEMKRMNHAGRHGEIKFEGDGRRGNYYKQVK
                     VYYAYQALDAKVATDAGSRNRGFTGTVQYRYRIYESERRPTQAEAATLTADKETSEEG
                     REIYRYNFSSGGVWDGAAGEKTKG"
     gene            11644..13311
                     /gene="recN"
                     /locus_tag="PKN92_03185"
     CDS             11644..13311
                     /gene="recN"
                     /locus_tag="PKN92_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNZ47828.1"
                     /translation="MIESVHIQDYALIRTLTIEFGKGFNVLTGETGAGKSIVIGALSL
                     ALGARASSEVVRRNAPKAVIEVVFRIDSPSPALQELLDEGEIQLEEDALILSRIVSSD
                     GRSRALINGRPVTITLLSSIGDELVDLHGQHEHQSLFRTDCQLQLLDAFAATTPQASA
                     LASLVSARNRIRRDLDNLLASSRDQVRHADFMRFEIQEIDDASLVPGEEEELKERLKL
                     ITHAEAIRSLSAEAYSRLYDADGSSAVDALDGTLNAVDKLCDILPDFKLILNELTEVR
                     ARIENVADEMRKHSREQEYDPEEINLINERLNLIRALKRKYGADISAILAYREKTAAA
                     LSDFDHQDERIDALRQEEVKLSAQAEKEAAALSLARKKAAQRMDREITNALHRLDMPH
                     AHFETAFETIPLAARGLDRISFLLAANAGEEAKSLKQVASGGEVSRIMLAIKAVFAHY
                     DLIPTLIFDEIDAGIGGATARRVSDRIRELAASKQVLCITHLAQIAAPADTHYKVTKE
                     EENGEQTTTQVFKMNKEEREKEIARLLDGSVSSASLRHAKALLKEFQ"
     gene            13441..13992
                     /locus_tag="PKN92_03190"
     CDS             13441..13992
                     /locus_tag="PKN92_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47829.1"
                     /translation="MMGSAGGNMDEEILERCRTLGHAIAEEGCAILTGGCPGLPHAAV
                     IGCKEKGGLTIGVSPALSIEEHVEWYESPLDNIDVMIYTGAGLMGREIIGVRSCDIVV
                     IVGGRSGTLGEFSIAYDEGRMIAVLTGTGGVADHIQTILPVIEKPTGSRIFFDDDPRR
                     LIQRSVASFRADPPIIAPREAAG"
     gene            complement(13993..15933)
                     /locus_tag="PKN92_03195"
     CDS             complement(13993..15933)
                     /locus_tag="PKN92_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNZ47830.1"
                     /translation="MSHPGKALLSAQKVSKSFGAQQILDNVSLTIHEEDRIGLIGRNG
                     SGKSTLLRILAGAVMPESGFTTRAQGIHVSFLEQQQHFESEEKVQDALNRAVAKWRSL
                     LQRWHQLNEQMASAEKGSREYLLWEAECQEIHHRLDVAGRWDPVVEIKLMAEQLHLPD
                     LKRTLGSLSGGELRRVDLARTLLEQPDVLLLDEPTNHIDADSVSWIEGYLEKYSGACV
                     LVTHDRYFLDRVSNRIVELSHGSVMSFPGNYAHFLEYKAAVDEVQQRTEANRQALIRR
                     ELAWYKRMPQARGTKQKARIGRLFDAAAQGPPPADKRFAFAIPEPERLGKDILEARLI
                     THAYGERLLFERFSLFMQKGMRVGIVGPNGCGKSTLLRVLMGQEEPLSGELLKGAATQ
                     FLYVDQTLADMDPELTVLQFISDGQRHVDVGRQRIHIPSYLESFLFDKSCADMPVGRL
                     SGGEKRRIDLAKKLLKGGNFLILDEPTNDLDLYTLRVLEETIEAFDGCALIVSHDRYL
                     LNRLCTHMLVFEEKGQIVQIAGNYDDYLLYRDRQETSVREERRNARKEESSEKSAPIS
                     RKKGLSYQEKKRLDMMESLIAEAEGALERLQKEVNAPGFFERPFTETQSTLTLLSNAE
                     QQVSDLFDEWMLLEAKKEEKDS"
     gene            complement(16046..17014)
                     /locus_tag="PKN92_03200"
     CDS             complement(16046..17014)
                     /locus_tag="PKN92_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ47831.1"
                     /translation="MGSKHDLNRRDFFRAAAVTGGAVLGAGVLTSPSPVEARAEKRYQ
                     EGVSPWPLVLNTSTIRPASLEEKIRVTAEAGWDGIEPWINELNAYEEGGGDLKELGAQ
                     IREMGLYIPNIIGLWDAMPATEDAFKESLKITRERMRQCAAVGSIHVAAIPAPDRPDF
                     DLAYGAKQYKELLKIGREEYGIKVAFEFVGFMKGVHRFGQACAVALDANDRDACLIMD
                     TFHLFRGGSGFEGLSKVQGSLFANFHWNDVPGDVPREEQGDEHRLYPGDGTLPLTQVL
                     QDLKATGYDKALSLEIFNRKYWEQPAEQVARDGLQKMRECVAAAEV"
     gene            complement(17046..18080)
                     /locus_tag="PKN92_03205"
     CDS             complement(17046..18080)
                     /locus_tag="PKN92_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47832.1"
                     /translation="MITPPDISRREFIVGTGRFSAALGIVAVCGNLLPQAAAGNGCIV
                     IGAGSRGECCLNVLLQCRYPVAALYDKDTGRGERLSRGYGIPSLDVAAPDRWAGRHPF
                     ILAVPPSEVLELIRALKMEDVPVLLDFSTESSVLHVLRILAECSLKKAAAVLTAVSPA
                     TEGLRRFIAQEDHGPLQRIHCSQGCCAPALLLEKHVRSLFMILSLCSNDEVSHHFSVY
                     SGCTGGQSDAYSTLMSSLSFASGLQAEFITTTADTVPLRMTFRGEKKRMRLDVASCAA
                     AMHCLSMEEVRIRSIHDWCATWYEKKCLESTPTTHATLLLSMLQQLGRSVPIAEISNN
                     IIFPTYTCKA"
     gene            18309..20045
                     /gene="lnt"
                     /locus_tag="PKN92_03210"
     CDS             18309..20045
                     /gene="lnt"
                     /locus_tag="PKN92_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNZ47833.1"
                     /translation="MAVSIIKKKYRPAAGVLLSALLLSLAFPRYHRYRLALFALVPLL
                     IHTKTGTAKSTLRRFFLCGWLFHTILLQWLWANIFWAGGWAVLAQQGLCLILALFWLL
                     PAGAWVYFRDAGGRYTTAIGFACFWVGMELLQARLFTGFGWSALGYTQGANRYVAQFA
                     SIGSVSLLSFFLVLINTLIAKIYTERQHFVLRILSVILLLGGLTAGGYFLFQEADYES
                     LPLNAGIIQPNFSQEMKWDSDYYGEMLRKTGNLTQSLVNHKKADIVIWPEALLTVDYK
                     IPLFHEALSDIARTAGTYIFTGTVRDDPSSGDSYNSSVLFDPNGKEAGCYDKIRLAAF
                     GEYIPLENYIPFVSRIAFGGITAGKEQQVFSLHGRKLAPLICFEVLFGPMAETLRQKG
                     ADALIVVTNLAWFGNSNAILQELEIARMRAIETGLPLLHSANTGVSGVFDPYGRFHTV
                     HHTVDSRGALLDWTEKTQPEHVVMKRFVDAFPVAAPARRLLPGGPVFLPWLLFGLGFL
                     LIAWRQFRQSSLFSLLEGETSEAQDVRESPADSGSVSAGTQTGAKAKAKAPPKKKAAA
                     RKPASKPKAKEE"
     gene            20075..20482
                     /locus_tag="PKN92_03215"
     CDS             20075..20482
                     /locus_tag="PKN92_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ47834.1"
                     /translation="MYQKEIPACLGSVSTKEPLPIYRQIVFLVALCLLSGTLQPGDQL
                     PSTRELARHLKVNMNTAARAYRLLKEKNMITVQRGVGVFLSDQAIEKGKAEAFAQIHT
                     AFRKSLADALKAGLSENEIFDLIRSLLAEPNRK"
     gene            20484..21236
                     /locus_tag="PKN92_03220"
     CDS             20484..21236
                     /locus_tag="PKN92_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47835.1"
                     /translation="MDSFIRPDSSTPESFSWVNGKDAWQIMPSCWQEGLPPAHTFKGD
                     NLLIVSTAISHFRDIEKSPAFQKLPMRQRRHLTNYSDEKSKRHKSMAYLMRAFLQQHF
                     GDTRMEMTTSHCGIWVVVALGQEVSGVDVEKLSSPAQYPSVAAKLFPPRWQAQLVQFE
                     NDAERESLFFTACWTALEARFKLEMAPALLPYLKAELQKSAPEKDTTVLNHFWVDEGY
                     IGCLARCKGGSIPLLHRLKADVILYPDQELPR"
     gene            complement(21218..21883)
                     /locus_tag="PKN92_03225"
     CDS             complement(21218..21883)
                     /locus_tag="PKN92_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ47836.1"
                     /translation="MADSLHTEIPLLLFDYDGVIADSFQVYFEEFLKACAQMGYGQFN
                     SRESFLKLFDRNLLVQALRAGFSLTQLKKLAVDFMPQMEKANARISPFPEMPAILATL
                     SARFPTIIITSNATAVVQKFVADHGLALIYDAIGSDIEPSKVKRIRRARRLFPAHKPY
                     YIGDTRGDMLEARRAGAVAVGAGWGWHDTARLRSGKAAHIVDTPAGLLEFFQRQIHYR
                     GSS"
     gene            complement(21885..23588)
                     /locus_tag="PKN92_03230"
     CDS             complement(21885..23588)
                     /locus_tag="PKN92_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNZ47837.1"
                     /translation="MTRYEEKPVIVQSDRSILLETDGPLFEEARDCLAAFSELVKSPE
                     HIHTYKLSPLALWNAASLGLSATEVVDSLKRFSRYEIPQNILADIQDQMSRYGRIKLY
                     RKDDGGLVLSADHPLLIRELLSHRQIKDLVFPAEDTHTLSVKAENRGNLKSALIKIGF
                     PVEDLAGYADGAPLEIAVRETTLSGRPFGLRQYQKESVDAFYLNGSNQGGSGVIVLPC
                     GAGKTIVAIGAIAAVKSHTLILATNTVALRQWKSELLDKTTLREDDIGEYSGDKKEVR
                     PVTIATYQVLTYRKTKNSPFTHFDLFDKALWGLVVYDEVHTLPAPVFRATASIQAKRR
                     LGLTATLVREDGQEEEVFSLIGPKKYDVPWKVLEKQGWIAEAACAEIRVPLPDNLRFE
                     YAVAPKRNKFRIASTNPEKIVLCEKLIESHKEDNILVIGQYLSQLKEIARHFKAPIIT
                     GQTPVGRRQELYAVFRKGDIKLIIVSKVANFAIDLPDANVAIQVSGTFGSRQEEAQRL
                     GRILRPKVSASPQACFYSLVSKDTCDQDFALKRQMFLTEQGYQYRIVEAARLDEFMQG
                     E"
     gene            complement(23719..24747)
                     /locus_tag="PKN92_03235"
     CDS             complement(23719..24747)
                     /locus_tag="PKN92_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47838.1"
                     /translation="MTRSVRVISVFLALSLFFGLAVSFLRLADTQRHALATDEVLYLP
                     NEKLLTYFTAGLNCVIADFIWLHCIQYTAHEHRGKRVFKWMEHMLTTATRLDPYYVAV
                     YRFGAMFLAALRADPEASLSLAYAGISINPHAWQLPYEAAMVYLLNKGEEPNSKYLAT
                     KLLTLATATGRAPGGIVNLTAKLQDEFNLLEVEQGMWQEMAASPDSFLSELGQRKLIE
                     IELRKICGVLDKQAQRFYQEKGRYPASLETLLQAGIIPYLPEDPLGGTFFLDAAGKSY
                     NTTLLEDEVIRARNTLINAVDRYHAKHIAYPTSLQALVEAGELNEIPAHPYPGKHWEY
                     DPFSGEVN"
     gene            complement(24744..25508)
                     /locus_tag="PKN92_03240"
     CDS             complement(24744..25508)
                     /locus_tag="PKN92_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ47839.1"
                     /translation="MNRIVTIAQNTFRESIRDKVLYVLLFFALATILGSKVLGWISIG
                     QDIKIIKDISLAAMSVFGALIAIFVGATLVYKEMDKKTLYTILSQPMKRYEFVTGKYL
                     GLVALLGVSLIIMAAGSTLYLKIAGAAIGLIWFQAILLIYIKLLLITAFAILLSTVVS
                     PILGALIVFSFYVGGHATDVLRDLPPQFDGTRAKQVLETLYYVIPNLNLFNVQSEAAN
                     MVPVTWPYVFYAVAYGISWTLFLLILACLAFERRDV"
     gene            complement(25501..26460)
                     /locus_tag="PKN92_03245"
     CDS             complement(25501..26460)
                     /locus_tag="PKN92_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ47840.1"
                     /translation="MSAIVTHDLSKTYKSLGKATVHSLRNVNMEVQENEIYGFLGRNG
                     AGKTTAIKLLTGLIQATKGRAEIFGEDAVLPSARARIGYLPEHPYFYEYLTPRETIAF
                     YGKLRGLSKDAIRKEWLRISEMLFLDEFADRRIRGFSKGMRQRVGLGVALIGNPPLLL
                     LDEPMSGLDPYGRRMVREIMLRLRAEGKTVFFSSHILTDVEEISDRVGILVSGVLAAS
                     GRIKDLTAGAQKAVELCCSGLKDEDVNAITARGIECRRGEEHQVTFILPEVAEAPPLI
                     AEIQQKGGMLLSLIPVLESLEDFFTKLQQREQNPSGKDGADHE"
     gene            complement(26482..27180)
                     /locus_tag="PKN92_03250"
     CDS             complement(26482..27180)
                     /locus_tag="PKN92_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNZ47841.1"
                     /translation="MARMARFVLGGYVHHVVQRSCLKKPIFTADQDFEKYIGLLAKHA
                     KLERVDIWAYCILPDSIHLLVVPPNARALSNMIRDIHATYSGYYNALNGHSGTIWRGR
                     FQSCVLDDPLVEFAARIVEFLPCRQRLAERAEDYRWSSAAAHTTENEHPFLTAHFPSP
                     EMRSHWSAFLHTNPQADLLEEELQMRTRTGRPFGRAAFFQELEQKYNRSLAPKKRGRK
                     AKPPKTNTAPDQNS"
     gene            complement(27229..28494)
                     /locus_tag="PKN92_03255"
     CDS             complement(27229..28494)
                     /locus_tag="PKN92_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47842.1"
                     /translation="MKKRLSIALVGAGMFGGDVHMRAYCQFAQYGFLPWLGRLGLDSM
                     ARALGDIEIDFVALGTRTETSAKARLAEYRALGLDFAIYHGETPWVDILRDFPDLDVL
                     AIATPDHLHTAPALAALEAGAHVIMEKPMALDAAEADAILAASKRAGRVVGVDMHKRY
                     DPDHLRIRDDVLHKIGEPLYGVALLEEPLAVSTEVFKAWAEKSDPFSYVGCHWTDLFI
                     SMMDVKPVSLHAVGQKKKLRQEYGMDAFDAVQVKIQFDNGMSIDFHNNWILPDSFEAP
                     VNQESRIFGTHGLVESDTQYRGLRFVSEKDGMRTANVHMTRNVLRPDGSLAYCGYGVD
                     SVAVCLEKIAEVKFLNKTLEDVEDYYPNAKQGRLSVLIVHAAREVAARNFACLESGKG
                     APVTALFNESGITIVDPYAGNEVIYSCPV"
BASE COUNT         7148 a         7830 c         6974 g         6941 t
ORIGIN      
        1 ccatgaaaaa ggcattggaa gacgctcgct gatactttca tgcgaccacg gagacggtaa
       61 ccatgcacat tcgcttcgaa aaacaaacgg ctttgctgct gttggtaatt ttttcggtct
      121 ttttatgcct tggcggcgga gcctattatt atttttttat cgcaccgcgt tacggcatct
      181 atcacgaaga ccttctggca ccggacacgg cactgcgcgt agtttttaaa cctgtcctgg
      241 ttcagaaata catcagttcg cttgccccgg cagggtcatc gcttattccc ggaattccca
      301 aagtaagttc catgcaacag atgcatttcc gcacggaatg gatccataaa atgccttttg
      361 agttttcttt tctgctggat caacgtgcat cggatcagct gggcgttcta ttttttgtta
      421 aagagcatcc ggcatcagag tccttttccg aactggtgga tgacggtggc ttctttcgtg
      481 ccacccgccc catcgtctgg gaccagaacc gcatgtcccg ctatgccgta agaacgctta
      541 cagcaacggg aagcgttctg attccccgca cgtatcagga agaaacagca aagcaccacc
      601 ccgtgtactc ccctcttacc cctcctgagg ttaagggaca tcatttcctc gaagctgcat
      661 taaataataa aaacggcaca ctaatagaac tgctcggcgc tttttccgca ccgttgcgcc
      721 ccattctttc gacagataca gaaaaaacgc tgatttccct ttcgccgaac attgaaggcg
      781 cttttttaaa ggcattttta tccgaggcgg atttactgct catcgaagta acagtcgagt
      841 gcagtgacag ttcttcagca gaagaagttt ccaaggccgt tcttgcagcc gcttatgata
      901 cagctgcttt tgcaaaaagg gaagggtttg cgctttactg ggacagcgac attgaggaca
      961 accacgttct gctgacaggc gagttttccg attttgagcc taaaatcagg cgcgccatgg
     1021 gcggataatc cgtcttccct tcagacgata ccccaccgac gtgcccggaa aaggacatat
     1081 aaaaaacggc gcatctttat cccgatacgc cgtcgcaaaa aaccggagaa gcaacgcagc
     1141 gattaaagcg ctttaatcca gacattgcgg aattgaacat aatgatttcg gtgggactgc
     1201 agtttcacag accccgcttc acgaggcggc ggctgcagac gatctttctc cttccacccg
     1261 gtaatccagc ccagctcctg ataattatga atcagaatgc cgttgtggta tacggttatg
     1321 cgcgggttgg cgagaagagt gccgtcatcg gaaaaacgtg ctgctgtata gcgaatgtca
     1381 taactctgcc actctccggg cggaaggcag gcatttacat ggggtgcact gagtttatag
     1441 agcgcgccac attcatcata ggtgccttca agaccgaaac tgtccagcac ctgtacttca
     1501 taggtatcct gcagaaagac gccgctgttt ccccggctct gccctttttc agtgggctgc
     1561 agcggtgtac gaaattccac atgcagctgc gcatccagat atttgttttt gctgacgaga
     1621 tcttctgtgc cgggcataac catcatggca tcgccgacga tctgccagcc ttcggagccg
     1681 gtaaaggcat caaagccagc accatcaaaa agcacgtccg ccccttccgg cggcggagtc
     1741 ccaagtccgg gattttcgta aatgaaaggc ttcatggaaa aagtggagga accgctggaa
     1801 cgaccgcctg taagtacacc gttttcaatt tttacccggt aattagcatc ggaaaattcc
     1861 agcacaccat tttttactct ggactccata acagcaagta ccgggcagcg cataaataat
     1921 ctggaagcca ggcgaagcgt atatttatta cgcccggtag ctgtgacttg tgcacttata
     1981 tccggatcaa catcttcatc gtcgctccag cgtccggtcc agttgcctgc ataagggtct
     2041 ctttcctgcg gataaaaatc atccgcacct ttcgtttcac gggcacagag aaccgggcaa
     2101 acaagcagca tcagcaccaa accgcccgct attaacgtca gaaccgcttt attccgataa
     2161 aaagcagaca ctttcatata aactccttta taggatcctg tttttactat atcttttcat
     2221 gaatgaaaat catgaagaaa ataaaggcaa ggctcaggcc cgccatgaaa agatcaccga
     2281 taatgcctcc gtgcctgcat ccgcatccag ggcattcatc gtccggatcg acatcctcac
     2341 cttctgttac aggatcttcg ccttcagtat cgggctcgcc ttccaccact tctccctcat
     2401 tttctccttc gcccggggcg acagcttttc gaacctgcgc tgtacgcact gccatggcac
     2461 gattgcctga agaatcacgg acgaaaaatt caacgtggta cacgccaacg gctgacgtat
     2521 caacgtccga ttcaaaaagc acatctttcc gcaggtcgcc atcgcaatca tccagagcgg
     2581 acaacacgcc cggatccaca tatacactgc cttcatccac gattacttct gcagcgccgc
     2641 gcagcacaat gaccggtttt ttcgtgtcaa ctacgatcac tttgcgggca acatccagcg
     2701 tgttgcccag ttcattggtg gcactgtaga taatgacata ttcgccgggc acagacagtg
     2761 cttctttcgg cagtttagtc gccgtaacgg gatactcaac agttccaatc tcattggttg
     2821 ccgttgcgcc cgggtcagta aactggtcac cgcactcaac ggtcacaata gcagcgccta
     2881 tcagttttac aatgggcttt tcaggattaa atgtaactgt aacagttcgt tcggtcttgc
     2941 tttcgttgcc ataaatatct tgtaccgtat agataaggat ataggtccct tcctcatttt
     3001 cattaacagt acccctcaca cgtactgcgc tgctaaggtc acctatacag gcatcaacag
     3061 cagtatatcc tggttccaca aagggcgtgc ctacatgaag atccatcgcc tcagcgcctt
     3121 ccaaggtaag gacaggcccc ttagaatcga taacatggac tgtgcgcact ttcgtccaat
     3181 cgtacaattg atagcggatg gtgtattctc cgggcacctg tgtattgaca cttccctgga
     3241 gatagtgcac aacaacttca tcacgtaaat catcgacaca gctgtcgctg gctgtgaagc
     3301 cgggctcttg gtatggatcg ccgcattcca caaccatcgt ggcactgccc agaagtttaa
     3361 actccggcgt tccacagccg acacggatca atcgtgtttt aacggcttca ttacccaaag
     3421 aatcggaagc gcggtaggtg acaaagtatc ttcctataac ggaagtatcg acagcatcca
     3481 ctccctcaac ggatacagag actgcacctt cacaggcatc aaaggcgtca gcgccactct
     3541 ccgtataatt atcgcctaca aggaggctca ccgtttcctt gccattgagg gtaatcacag
     3601 gcgcttttgt atcgactact gtaattgtac gggtcacttg tgcctgtaat cccatataat
     3661 tggttacttt gtaagtccgg cgcaccgttc ccgtactggc aaagcttccg tcatccgtat
     3721 actccaccgt atcattaatt tcatcttctt cacactcgtc ccatgcctga acacccgggt
     3781 cagcaaaaat ctctccgcat tccgcctcca tcacggcatc gccattcaat tcgattacag
     3841 ggccgggcga aacacagccg acacggacag tacgaacagc ttccgccaca ttgccggcac
     3901 tgtcctgcac actgtacctg acggaatagt cccctgcttc cgatgggtta agttcgctgt
     3961 catccaccag aatgctgccg gtaagatcac catcgcagct gtcggaagcc gttgccccgg
     4021 gatcggtaaa agtaccgttc aacggaatat ttataatttc acgacccagt aattggagag
     4081 caggtttaag ggtatcgaca actgtgatct ttcttgttac ttccgtttta ttgccctgtg
     4141 agtttgttac tgaataaagg cgtgtatact caccggggct cttcgtatta acattccccg
     4201 cagcaataat ttgtgaagtc agatcgccat cacactcgtc atcggcggca gcacccggat
     4261 ctatgaagga agtgccgcat tctactgtca tctcagcatc accgaggagc gcaattcgcg
     4321 cacctgcaag agaggcataa caaaactgcc gaaccacgga cgaactaccc agactgtatc
     4381 tcagttcata tttcgcagcc ttctgattaa atcccttttc attaaattct gaacctatat
     4441 gtgccagata ggtcgtatga gctgcgttca gcgaaggcaa aataaaaagg cttaccttga
     4501 cttttttttc ggcatcatcc aaaggcgggt gaatcgacaa acccgcctcg aagtacacat
     4561 cacccaccgt ataccgttga ttcggtgcac cgttcaaggc taaagtatac ccttccaggg
     4621 gctcggactg cgcctgggtg taccctacaa tcccgagcag tccaatacag aaaagagcgg
     4681 ttaacacaac taaaagaatg ggtgttctca tggcatgtat ccttgtttca aaggcggtat
     4741 acaattctat tacagaacct gattctgctc ttggaagcag actggaatga tttccttcgc
     4801 ccactaatac tatacaattg ctattgcaat acgcaacctg ttttccagaa aaaccgctct
     4861 cttatccaat ccgccagtcc atcacagcat ttccgggctg gcacccgcat ccggatttta
     4921 cagcgattcc attaatcggg cgcaattcag caccttttct acgagatccg ccggagtata
     4981 gaaataatga tttgtcgctt caaaccctat gcgtgaatcg tagcgctgca gttcatacag
     5041 agaacgggcg cactccagtt cctgcctcag cagttccttc atttcttcac gaatcttctg
     5101 ctgtttctcc ttattggctt ccttcgccag ttcattccgc tgaaggataa atcgcccctg
     5161 ctgcgcaata ctctgataat ggagagcagc tgcctgcgca agatgcattt cctgctgaac
     5221 cgctgtggca tgaacatttc ccttttccaa cgcactttcc atcagcagtg cactctctct
     5281 aaaaccggac gccacttttt ccagttgatc aagaaaagta ccagccggat aaaccacacg
     5341 ccaggcatcc agatcatcat aaggaagccc gaccatggtc gctttatagc cggtgggctc
     5401 gcgccacaga agattggcag gccctacctg caacggtgca ttgtaaacga cccctccatg
     5461 ataaggaaat tctttaaagg catcgctcag ctttttccag gcgtgaacca tggttccggc
     5521 agcgtcgctt ccgaagcgtt tttcggcgac actgtgaagt gcctcatcgg ctgtgcggaa
     5581 ccgtccgtcc gcgagcggtt caccaagagc atacaccacc tccagattcg gtgacggata
     5641 tccgcccaga gtccatccga gcatcagtcc ggaaactccg ctttccctca gatgcgtaat
     5701 atgctccgcc acatttgcca cagccggaat atatggaagt gctcccagtt cccaggtggt
     5761 gtttgcctgg attttcgcca cggcacccag cccggcattc ttcgccgcct cccattgccg
     5821 aagggcgcgt ggtcccggtc ccaccactga aagagaatat tcgcccactt tggtggacac
     5881 accgccgacc gatacaggag catcccattc actgacgctc ataagggatg ctctgcgggg
     5941 cagcaaagca acacactcct gcgcccaggg ctccgcccat ccccagtccc acgccagaag
     6001 acgggcattg ctgcctgccc gatccagccc cgatgcaata aaagtgttca cgtctgcgat
     6061 tgtttttgcc gcaccccgtt cagcacatcg tgaacacatg tccccccgat gatgagacca
     6121 gcagtttgtc agattttccg atgcggtaat cgtcaatatt ccggcaagcg caggggctgc
     6181 cgacgcaatg gatgccgtgg catcggttat gtaggacgcc acttccggcc gggcagtaca
     6241 gagcgtggca tatccgtttt cctctacccc tttcaaatca gggtattttt caaaaaatgc
     6301 cagaggcatg gcgcgcggct cgttaaagta aagataaata ccgatgccga aacgggcagc
     6361 ccgttccgtc atggcacgca gtgattcaag gcgctgttcc catccttcgc tgagctcggg
     6421 agcccagggg aaggggctta atttgtataa gactgcatga agccatacgc cgttgacgcc
     6481 actttcggcc aggcgctgta aatagccatc cggaaaaggg tcctgttcga gaaaaggatc
     6541 gccatagagc gcaaagtagg aatagcagaa gcggggattg aaccggttaa tcgcttcaac
     6601 cggctcacta tgcgtcacag gctcgcatag atcctttata aaagaaaaag gcggtgtctt
     6661 ccaggagcct gcctcttctc cgaaatattg atgtaccaga tccttaattt cttcgacccg
     6721 caatttgaca gaagcatcgg gagcatgata ttgaagtgga gcacagacag gcttaaatcc
     6781 gcccaattta atcagcagaa aatcatcttc acgcagcgtg tatgccagct tttccgcgtc
     6841 ccactccagc agggtcagca gctgatcgaa agggagcagg tgccaattgg cccgaataat
     6901 ggtaatataa ctgcgcgccg attgttcttt tgaaatgtcc ggagggtgcg gcaatcccat
     6961 ggagcgcccg gtctccagaa tctgtgacgc atcggtgccg actgccgctg ccatgcgctc
     7021 caaaggaacc aggttccagt tgcgccagac aaaggcatgg agccggtccg gaaaatgagt
     7081 aaacgccagt gcttccggcc cagtctcagc aggaagcacg gcagccgtgt agaccgcaag
     7141 tgcaagaaag agagcatgca tccgaccgcc ctttccaggc aaagtagttt tgattggtgc
     7201 aattatagca gaatgcgccg tttctgtaaa tagtattccg tccgagaaat tgccgattcg
     7261 gtagaaaaca aagacgattg atcgtgtcga aaccgcttcg accagaaagc caaacgatga
     7321 aaaaactgta tatccacgcc atcgttcttc ttgctgcggg aagctatctc gctcttttca
     7381 gtgcctacgc ccagccctct gctttttacc cggagcagat cacacccgcc gaagtaacaa
     7441 agcctgcgcc atctgaccag acttcatcgt cctctggaac ggcagaacct gcagaagatc
     7501 ctgtggaggc ttttatccga aaccggtcat tgcaacagag aatcgctcag ctgatgattg
     7561 tcactctcga aggagaaagc cggcttttac tgggcgacgt tacctatctc aagtcattca
     7621 gccccggcgg gatcctgctt gcccggtctc tgaatccgag cactgctcta agttatgttc
     7681 aatctgtccg tagttttgaa gccgtctcag gcgccccttt tctcatcggg gcggatcttt
     7741 ttgcgctcac ccagcccaca cgctctcttc cgtcccagtt cattcagttg ccgacacctt
     7801 tgtcagtgct tgccacatcc agccccgaca tttttcagga ttatagtgct ttactggcgc
     7861 tttacacaga aagtctcggc tttaattttt atcccgggcc tgcgctggat ctctcccctc
     7921 cttttaaaac agcagcggac accgtgctca ctctgggcag tgatcccgta aaagctgcag
     7981 gcattgcctc atgttttcaa aacgcttttg caggcaccag aacagcctgg atgcctatgg
     8041 gttttccggg aggaggtgcc aatagagaag gacggagtgc ggctgtactg accacgcctc
     8101 tgtccgctct tctggaacag gacggactgc cttaccgcca ggtgatcgaa cagggtgcgg
     8161 gtatcattca tgtggggaat gtgctggttc ccacactgga cacttcgtct cctcctgcga
     8221 gcctttccaa acaggtaatg actgatctgc ttcgccatca gttgaacttt cagggcgtta
     8281 ttgccgccgg gcctctggat gaagcattga ttcaggcgga aaccgaccct gctgtttcag
     8341 ccgtgatggc attgcttgcc ggagcggata tgctgctgtg gcaaagtaat ttagtacagc
     8401 cggaaaaggc aaacaagcgt attgccgccg ccgtcaccga cgggacattg tcggaagaac
     8461 gtattaatga ttccgtgcgt cgggttctcc ggctaaaaat gaatctggct caggcaacgc
     8521 ctgctcagct caccagtaaa aacaaacggg tcgacctgaa aaagctcggt gaatggagcg
     8581 tgaaagtgga gcgccatgcg gcaactctga ttaaaaatca gggcaacgca cttccgctgg
     8641 ttaaagatgg aggcgcaccg gtcggaatta caggtgttgt cggtgtggaa gagctgtatc
     8701 agctcctcga aaaagaagtg aagcgccttg tcaggcaaag catcacgagt gcgcgccatg
     8761 tcggagacat cgaacgtttt gaaattgaca ggctgacacg caacatgaaa gcactgggca
     8821 ccattgtggt gatactgacg gatcgggttc gctttgaaag ccagatcgag ctgctccgcg
     8881 ccctcaagca gaacggcgca aaacttgtgg ttctctttct gggtgacccg cgcaatgcct
     8941 gccgattgca ggaggcggac agcatccttc tttcctattg caattccaat gcaacgctgc
     9001 aggcaatgca ggcactggcg ccggtactga tgggtaaaag cccgcttcgt ttccgagatt
     9061 tcccggacga aatcagagtc aaagcaggcg aagaacggag ctttgattcc aaagaaatac
     9121 tctgttcgcc ggtgggcagg cttcccattg cactctccga ccagtatccg gcaggctttt
     9181 ccctgtctat ggatccaggt cccgccatta aaaaagtgga atggtttttc gacggaaaac
     9241 gcatcaaaaa ggacaaggta gcgtttacgt tttcaacacc gggaaagcac acagtatcag
     9301 tcactgtaac cgatatgttt aaaacggagc aaagccggga aataccattt tacgcggaat
     9361 agaagattca ttataaaacg gcattgatca ccgcctgaat gtcggtagca gaaagccccc
     9421 gcccatccac atccgcatca aaggacgttg cttttccaag tgcggcattt actaccgcct
     9481 gtacatcttc agcatccttc gtgccgtcct gattcacatc tccgtccgat agaaaaatgt
     9541 cttttctcgt cgcgccgccg gactctacag caatggcatc tgcatggtac gttatgtatc
     9601 cgggtgcctg atacgtgagg ttataggtgc cgggcaacag caagcggtga tagtttccta
     9661 cggcgcggct tgtaaaaacg ggctgtgccc ggtcatgtgt cattactttt gcccagagag
     9721 gcagcttcgt cgtgcggtct tttaccagcc cccgtatccc catatgcacg gcactgagat
     9781 aggcatacat ggcatcgcgg ttttcatccc agaaccgggg cagctgcgat gcgggcgggc
     9841 gtttgtccgc cgatatttcc agagtgagct ccgggcagcc caggaaacga taatgccagt
     9901 ccatcatgcc gccggttatg ctgtaccagg cggagccgtt aataatcccc tgcggaaaaa
     9961 tcgggctctg atacatgggc ggattgtttc gcgaatattc cagagcaaga tagcggaaaa
    10021 catcatcatc gggactgata gccggggagc cgcttgaaat cccgggcgtg ttatcgtaag
    10081 gatagttcgc cagaagtgca ccgccatgaa agttggcgca taaattggcg gggtatcgaa
    10141 cgtgccaccg catgatgcag ccatcttcca cagggacttt ttcatcgccc agaggctcac
    10201 catcgaaaag agttgtgctg taatgctgcc cgtaagcggg aaagtttctg ttgagatcct
    10261 taccggcgct gtatcgcgaa accgcagcat gcccatccgg gttcatgata ggcaccaccc
    10321 agattacagt tttgcagata aggtcactta taaaaggatt tttgccgaaa ttctcaagca
    10381 aatcctgaat gaaataaagg cacatttcaa cgccgacaat ttcgtctcca tgaatggcag
    10441 ctatgtattt cactacaggt ttatctgctt cctgctccgg tgtcagcgtg atttttatcg
    10501 cccagatttc ccggtactgc ggtgtctgcc ccaatgtccc cgtctgcact atattgggat
    10561 actgattggc gtaagagctg aacagcgggc ctatctggtc ataactcgta taccccgatg
    10621 ccttttcggc cgatgacccg tcgggtatcg tttcaagaag atgcagcggg taagacaact
    10681 ttttaagctg ctccagctct tcttcattta catagaaagt gaccttccgg tcaacacggt
    10741 tatcaatagt caatccgcag ttaagcagat cagcaagagc gacttcgtcc ggagccgtca
    10801 cctccacgat gtagaagggc gaggcggaag cgcatacgga cagggcaaga acacagacaa
    10861 acatgaaaag cggacccctg ccgtaagatg tccggggcgt tacattcatc ctttggtttt
    10921 ttctccggcg gcaccgtccc aaacacctcc ggaagagaag ttataccggt atatttcccg
    10981 tccttcttct gaagtctcct tgtccgctgt gagcgtcgcg gcttcggcct gggtcggtct
    11041 gcgttcactt tcatagatac ggtagcgata ttgaacggtt ccggtaaacc ccctgtttct
    11101 gctgccggca tcagtggcaa ctttagcatc cagcgcctga taggcataat acaccttcac
    11161 ctgcttgtaa taattgccgc gccggccatc gccttcgaat ttaatttcac cgtggcgccc
    11221 ggcatggttc atccgtttca tctcggtggt gcccacaaga tccagctgtt caaaggccat
    11281 gtctgttcgt gacacatgac cttcaggatg ttctttcaga ccgaaatcag ccagcacttt
    11341 gtctgccacg cttcccccgc cggtttggca gcctgataaa aaaagacaca agaccaggcc
    11401 gctgacacac gctgttgcct ttaatctgga aagcatcgta tttcctgaaa agatttccat
    11461 agcacaactc ctgttctgca ttgaaacata tgcagattct atagtttcag tataccatga
    11521 ttggcagcat caatacagcc cgaacatcac gctctgccat aaagcgcaca tctgtgatac
    11581 aatacatttt gaaggcgaag tcatttattt tatatttctt tcatccggct tttattgctt
    11641 tttatgatag aaagcgtgca cattcaagat tatgccctta tccggacttt aacaattgag
    11701 ttcggaaaag gctttaacgt gctcacaggc gaaactggcg caggaaaatc cattgttatt
    11761 ggcgctctga gtctggcatt gggcgcacgg gcatcttcgg aggttgtgag gcgcaacgcc
    11821 cccaaggcgg tcattgaggt ggtgttccgc atcgacagcc cttctcccgc tttacaggaa
    11881 cttctggacg aaggtgagat tcagctggag gaagatgcat taattctgtc gcggattgtt
    11941 tcgtcggacg ggcggagccg ggctttaatt aacggcagac ccgtcaccat cacattatta
    12001 agcagcatcg gtgatgagct ggtcgacctc cacgggcagc atgaacatca gagcctattc
    12061 agaacagact gccaactgca gcttctggac gcctttgccg caaccacgcc gcaggcatca
    12121 gcgctagcct ctctcgtatc cgcacgaaat cggatccggc gagatctcga caatctgctt
    12181 gcaagcagca gagatcaggt gcgccacgcc gatttcatgc gctttgaaat ccaggaaatc
    12241 gatgacgcct cccttgttcc cggcgaagaa gaggaactca aggaacggtt gaagctgatt
    12301 acccatgccg aggcaatcag atcgctgtct gcggaagcgt acagccgcct ctatgacgct
    12361 gatggctctt ctgcagtaga tgcattggat ggcacactca atgctgttga taaactctgc
    12421 gacattcttc cggactttaa attgattttg aatgaactga cggaggttcg cgcacgtatt
    12481 gagaatgttg ccgatgaaat gcgtaaacac agcagagaac aggaatatga ccctgaagaa
    12541 atcaatctga ttaatgaacg actgaacctt atcagggcgc tcaaaagaaa atacggcgcc
    12601 gatatttccg ccattctcgc ctatagggaa aaaactgccg cggctctctc tgattttgac
    12661 catcaggacg agcggattga tgcgctgcgg caggaagaag taaaactctc cgcccaggcg
    12721 gaaaaagaag cggccgccct ttccctcgcc agaaagaaag cggctcaacg catggacaga
    12781 gaaatcacga atgcgctgca tagactcgac atgccccatg ctcattttga aaccgccttc
    12841 gaaacgattc ctctggcagc acgggggctc gatcgtatct cctttctgct ggcggcaaac
    12901 gccggagaag aagccaaatc tctgaaacag gtggcgtcag gcggtgaagt gtcccggatt
    12961 atgctcgcca tcaaggccgt ttttgcacac tacgatctga ttccaacctt aattttcgac
    13021 gaaattgacg caggcatcgg cggtgctacg gcacgccgtg tatccgacag aatccgggag
    13081 cttgcagcct caaaacaagt gctgtgcatc acgcatctgg cgcaaattgc cgccccggcg
    13141 gatacgcact ataaagtgac gaaagaggaa gaaaatggtg agcaaacgac cacgcaagta
    13201 tttaaaatga ataaagagga acgggaaaag gaaatcgccc ggcttcttga cggctctgtc
    13261 tcttctgcca gtttgcgcca tgcaaaggcc ttattgaaag agtttcagta aagatgtatc
    13321 atacaagttg gtctaatcac tgaggagaaa aagatgcata aagatcattc aaagtccaga
    13381 caaagaaacg gggcgccacg ggttccgatc tgcagaacct gcgggctgac aaagatcggt
    13441 gtgatgggct ctgcaggagg aaatatggac gaagagatcc tggagcgatg ccgcaccctc
    13501 gggcatgcca ttgcagaaga aggatgcgcc attcttacgg gcggctgtcc cggacttccc
    13561 catgcggcag tgataggctg caaagaaaaa ggggggctta ccatcggtgt ctcgcctgcg
    13621 ttgagcattg aagagcatgt ggaatggtat gaaagcccgc tggacaatat agatgtgatg
    13681 atttataccg gtgcagggct tatgggcaga gaaattatcg gagtgcgcag ctgtgacatc
    13741 gttgttatcg taggcggccg ttccggcacg ttaggcgagt tttccatcgc ttatgacgaa
    13801 ggccggatga tcgctgttct tacaggtacg ggcggtgttg ccgatcacat acaaactatt
    13861 cttccggtca ttgaaaagcc aacagggagc agaatctttt ttgatgatga tccccggcgc
    13921 ctgatacagc ggagcgtcgc ttccttccgc gccgatcctc cgataattgc gccccgtgaa
    13981 gcagcaggct gatcaggaat ccttttcttc ctttttcgcc tccagcagca tccactcgtc
    14041 aaataaatcg ctgacttgct gctcggcgtt gctgagcagt gtcaatgtgc tttgtgtttc
    14101 agtgaagggg cgttcaaaaa agcccggggc attcacttcc ttttgcaacc gctcaagggc
    14161 gccctctgcc tcagcaataa gagactccat catatccagc cgtttttttt cctgatagct
    14221 gagtcctttt tttctggata taggtgcaga cttttcactg ctttcttctt ttcgggcgtt
    14281 tcggcgttct tcacgaacgc tcgtttcctg cctgtcccga taaagcaggt aatcatcata
    14341 atttccggca atctggacga tttgtccttt ctcctcaaaa acaagcatat gcgtgcagag
    14401 tctgttgagc agatatcggt cgtggctgac aataagtgcg caaccgtcaa aggcctctat
    14461 ggtctcctca aggactcgca gcgtataaag atccaggtca ttagtcggct catcaagaat
    14521 caggaagttg ccgcctttca gcagcttttt ggcaagatca atgcgtcgtt tctcaccgcc
    14581 ggacaaccgt ccgaccggca tatcggcgca gcttttatca aaaagaaaag actccagata
    14641 ggacggaatg tgtatacgct ggcgtcccac atccacatgc cgctggccgt cggaaataaa
    14701 ctgcagcaca gtgagctccg ggtccatatc tgccaatgtc tgatccacat aaagaaattg
    14761 ggtggctgcc cctttgagca attctccgga caaaggctct tcctgtccca tcagcacgcg
    14821 caggagggtt gattttccgc agccattagg acctacaatt ccgacacgca ttcctttctg
    14881 catgaaaagg gaaaaacgct caaataacag cctttcacca taggcatggg tgatcagacg
    14941 ggcttcaaga atatcttttc ccagacgttc cggctcggga atcgcaaaag caaaacgctt
    15001 atctgccgga ggcggcccct gtgcggcggc atcaaagagc cttcctatcc gcgctttctg
    15061 tttggtgcct cgtgcctgag gcatacgttt ataccaggca agttccctgc gaatcagcgc
    15121 ctgtctgttt gcctccgtgc gctgttgcac ctcatctaca gccgctttat attcgaggaa
    15181 atgggcataa ttcccgggaa aactcataac agagccgtgg ctcaactcta caattctgtt
    15241 ggaaacccgg tcaagaaaat aacggtcgtg cgtaaccagc acacaggctc cggaatattt
    15301 ttccagataa ccttcaatcc aggaaacgct gtcagcatcg atatggttgg tcggctcgtc
    15361 gagaagcagc acatcaggct gttcgagaag cgtgcgcgcc agatccaccc gccgcagttc
    15421 gccgccggac aggctgccaa gagtccgctt taaatcgggc agatgcagct gttccgccat
    15481 cagctttatt tcgacgaccg gatcccatct gcctgcaaca tcgagccgat gatgaatttc
    15541 ctggcattcc gcttcccata gcaggtattc acggctgccc ttctctgcag atgccatctg
    15601 ctcattcagc tgatgccacc gctgcagaag gctgcgccac ttggcaacag cacggttcag
    15661 cgcatcctgc accttttctt cgctttcaaa atgctgctgc tgttcaagaa aagaaacgtg
    15721 tatgccctga gcccgtgtgg tgaatcctga ttccggcatt accgcaccgg caagaatcct
    15781 gagcagggta gattttccgg atccgttgcg accgatcagg ccgatacgat cttcttcatg
    15841 aatcgtcagc gaaacattgt ccagtatctg ctgtgcacca aaagacttgc tgactttttg
    15901 tgcactgagc aacgcttttc cggggtgaga catcaaattt catgtacctt ggagccgatg
    15961 cataatgtgc gctgcgcatc cttttgcatg cacgtctgcg cggtatccgc aaatgagcca
    16021 actgcgcacg cggcagaaag tgctcttata cttccgcagc cgcaacacat tcacgcatct
    16081 tctgcaatcc atcccgggca acttgctcag caggctgctc ccagtatttt cgattgaaga
    16141 tttccagaga aagcgccttg tcatatcccg tggctttcag atcctgaagc acctgagtca
    16201 aaggcagggt accgtctccg ggatacaggc gatgttcatc gccctgctct tcccggggca
    16261 catctcccgg aacatcattc cagtggaaat tggcaaagag gctgccctgc actttgctga
    16321 gtccttcaaa gccggaaccg ccccgaaaca gatgaaacgt gtccatgatc aggcaggcat
    16381 cccggtcatt ggcgtccagg gcaacagcac acgcctgacc aaagcggtga acccctttca
    16441 taaaaccgac aaattcaaaa gctactttta ttccatattc ctcacggccg attttcaata
    16501 attctttgta ttgcttcgcg ccgtaagcaa gatcaaaatc cgggcgatca ggtgcaggaa
    16561 tcgctgccac atgaatagaa cctacagctg cgcactgccg catacgttcc cgggtgatct
    16621 tcagagattc cttaaacgcg tcctctgtcg ccggcatcgc atcccagaga ccaatgatgt
    16681 tgggaatgta aagccccatc tcgcggattt gtgcgcccaa ctctttcaga tcaccgccgc
    16741 cctcttcgta ggcattcaac tcattaatcc agggctctat cccgtcccag cccgcctcgg
    16801 cagtgacacg aattttctct tccagcgagg caggacgtat ggtgctggtg ttcaacacaa
    16861 gcggccaggg actcacaccc tcctgatacc gtttttccgc acgggcttcc accggagagg
    16921 gtgatgtcag aacgcctgct cccagcacag caccgcctgt taccgctgca gcccgaaaaa
    16981 aatcacgccg gtttaaatca tgtttggaac ccatggtttt tcctttctca aaagacaaaa
    17041 ggcgatcatg ccttacaggt gtaggtggga aaaataatat tgttggaaat ttctgcgata
    17101 gggacagaac gccccaactg ctgcaacatt gacagcaaga gtgtagcatg cgttgtcggg
    17161 gtgctttcaa ggcacttttt ctcataccag gtggcacacc agtcgtgaat actccgtatc
    17221 cgcacttcct ccattgataa acagtgcatt gctgcagcac aggatgcaac atccagccgc
    17281 atacgttttt tctctccccg aaaagtcatg cgaagcggca cagtatcggc agtagttgta
    17341 ataaactcgg cctgcagccc ggaagcaaag gacaaagagc tcattaacgt gctgtaggca
    17401 tcgctttgtc cccctgtgca gccggaataa acagaaaaat ggtggctcac ttcatcgttg
    17461 gaacaaagcg aaagaatcat aaaaaggctg cgaacatgct tttcaagaag aagcgccggt
    17521 gcacaacaac cctgtgaaca gtgaattcgc tgcaaaggtc cgtggtcttc ctgagcgata
    17581 aagcggcgca gcccctcagt ggccggactt actgccgtta aaaccgccgc tgccttcttc
    17641 agtgaacact ccgccagaat ccttagcaca tggaggactg aagattctgt ggaaaaatca
    17701 agcaaaacag gcacatcttc catcttcaaa gcgcgaataa gttccagtac ttccgaaggg
    17761 ggcacagcga gaataaaagg gtgccgccct gcccatctat ccggagcggc gacatccagc
    17821 gacggaatgc cgtatccgcg cgagagccgc tcccctcgtc cggtgtcttt gtcatataac
    17881 gcagcaacag gatatcggca ctgaagcagt acgttgagac agcattcgcc ccggctgcct
    17941 gctccgatca caatacagcc attgcctgcg gcggcttggg gcagcaaatt tccgcatacc
    18001 gctactatac ccagcgcagc cgagaatctg ccggtgccca caataaattc tcttcttgaa
    18061 atgtccgggg gtgttatcat actgtatcca caagaacggg ttattggtat aagcggggta
    18121 catcacaacc gcatgaagac agggtaagca tgggctctgc ttacaatgac tactttaacc
    18181 gcttgcaggt gagggataca aatagccgcc ctcctggagc agaaaatcag aaatgattac
    18241 atctcaccag attgacaacg acagactaca catcagccag gaattcgcta aggatcggtc
    18301 aataaaatat ggcagtttcc atcattaaaa aaaaatatcg ccctgcagca ggtgtactgc
    18361 tcagtgcgct tctgctctct ttggcgtttc cgcggtacca tcgttaccgg cttgccttgt
    18421 ttgcactcgt tccgcttctg atacacacca aaacaggaac agcaaaatcg actctgcgac
    18481 gcttctttct ctgcggctgg ctttttcata ccatcctgct tcagtggctt tgggcaaaca
    18541 ttttctgggc aggcggctgg gcggtacttg cgcagcaggg gctatgcctt atccttgctc
    18601 tcttctggct tcttccggcg ggagcctggg tgtattttcg tgacgcaggg ggcagataca
    18661 caacagcaat aggctttgcc tgcttttggg ttgggatgga actgctgcag gcacgtcttt
    18721 ttaccggatt cggttggtcg gcgctgggtt atacacaagg tgcaaatcgc tatgtggcac
    18781 agtttgcctc cattggcagc gtttctcttc tttccttttt tctggtgctg atcaacacac
    18841 tgattgccaa gatctacacc gaaagacagc attttgtgtt acgcatcctc tctgttattc
    18901 tgcttctcgg cggactgacc gcaggcggct atttcctgtt tcaggaggca gattatgagt
    18961 cgctcccgct gaatgccgga attattcagc ctaatttttc acaggaaatg aagtgggatt
    19021 ctgattatta cggcgaaatg ctgcgtaaaa ccggcaacct cacccagtct ctggtaaacc
    19081 acaaaaaagc ggacatcgta atatggccgg aagcgctgct tacagtcgac tataaaatcc
    19141 cgctgtttca cgaagcactc tcggacattg cccgaaccgc cggaacctat attttcacag
    19201 gaactgtccg ggacgatccc tcgtcggggg atagctacaa ctcaagtgtg ctcttcgatc
    19261 cgaacggtaa agaagcgggc tgctacgata aaattcgtct cgccgctttc ggtgaatata
    19321 ttcccctgga aaattatatt cctttcgtca gcagaattgc cttcggcggc atcaccgccg
    19381 gcaaggaaca gcaggtattt tctctgcatg gaaggaagct tgcgccactc atttgctttg
    19441 aagtgctctt cggaccgatg gcggaaacat tgcggcagaa aggcgccgat gcgcttatcg
    19501 tagtgaccaa tctggcgtgg ttcggcaatt ccaatgctat cctccaggaa ctggaaattg
    19561 cgcgaatgcg ggcaattgaa acaggcctgc ctctgctgca cagcgccaac acaggtgtgt
    19621 ccggggtttt tgatccctac ggacgttttc ataccgtaca tcacacagtt gattcccgcg
    19681 gtgctttgct ggactggact gagaaaacac aacccgagca tgtggtaatg aaacgctttg
    19741 ttgatgcttt tccggtggca gcacctgccc ggcggctttt gccgggcggt cctgtatttc
    19801 ttccctggct ccttttcgga ttgggcttcc tgctgatagc gtggcggcag ttcaggcaaa
    19861 gcagtctttt ttccctgctc gaaggggaaa cttctgaagc acaggatgtg cgggaatctc
    19921 ctgctgacag cggcagcgta tcagccggaa cacagaccgg agcaaaggca aaagcaaaag
    19981 caccgccaaa aaagaaagct gccgcccgaa aaccggcgtc caaaccaaaa gctaaagaag
    20041 aataaggcct gctgtcttca gagatctttt cgccatgtat caaaaagaga ttcctgcctg
    20101 cctgggttct gtcagcacca aagagccgtt gcccatttac cggcaaattg tttttctagt
    20161 tgctttgtgt ctactgtccg gaaccttgca gccgggagac caactgcctt cgacccgtga
    20221 gctcgcccgg catttaaagg tgaatatgaa tactgccgcg cgcgcctatc gcctgctgaa
    20281 agaaaaaaac atgattacag tacaacgggg cgtcggtgtg tttttaagcg atcaggcgat
    20341 tgaaaaaggg aaagcagaag cattcgcaca aattcacaca gcattccgaa aatctttggc
    20401 cgatgccctg aaagcaggcc tttcggaaaa cgaaatcttt gacctgatcc gcagtcttct
    20461 tgcggaacca aaccggaaat aagatggact cttttatccg ccccgacagc agcacacctg
    20521 aatctttctc atgggtaaat ggaaaagacg catggcagat tatgccttcc tgctggcagg
    20581 aaggactgcc gcctgcacac acattcaaag gggacaacct tttgattgtc agcacagcga
    20641 tttctcattt cagagatatt gaaaaatctc ctgcttttca gaaacttccg atgcgtcagc
    20701 gccggcatct cacaaactat tcggatgaaa aaagcaaaag acataagagc atggcctatc
    20761 tcatgcgcgc cttcctccag cagcatttcg gcgatacccg gatggaaatg acaaccagcc
    20821 attgcggcat ctgggtcgtc gtggcgctcg gacaggaagt atcgggagtg gatgtggaga
    20881 aattaagcag ccccgcacaa tacccttcgg ttgcggcgaa attatttccg ccccgctggc
    20941 aggcacagct ggtacagttt gaaaatgatg ctgaaagaga gtccctgttt tttaccgcct
    21001 gctggacagc gctggaagca cgctttaaac tggagatggc acccgccctg ctgccttatc
    21061 tcaaggcgga gctgcaaaaa agtgcaccgg aaaaagacac aacggttctg aatcattttt
    21121 gggttgatga aggctatatc gggtgccttg cccgatgtaa aggggggagc atcccgctgc
    21181 tgcacaggct caaggccgat gtcattctgt atccggatca ggaactaccc cgataatgta
    21241 tctgcctctg aaaaaactcc agaagccccg ccggtgtgtc tacaatgtga gccgctttac
    21301 cgctgcgtaa tcgggctgta tcatgccagc cccagcctgc accgaccgcc acagcccccg
    21361 ctcttcgtgc ctcaagcata tccccccggg tatcaccgat ataatagggt ttatgcgccg
    21421 gaaaaagtct tcgtgcgcgc cggattcttt tgactttact gggttcgatg tcgcttccaa
    21481 tcgcatcata gatcagggca agcccatggt cggcaacaaa tttctgcact acagcagttg
    21541 cattggaagt aatgataatc gtcgggaacc gggcacttag tgtcgccaga atcgcaggca
    21601 tctcgggaaa gggactgatc cgggcatttg ccttttccat ttgcggcata aaatcaacgg
    21661 ccagtttttt tagttgggtc agtgaaaacc cggcacgaag cgcctgaacc agcaggtttc
    21721 tgtcaaagag tttcagaaaa ctttcccgtg aattgaactg accatagccc atctgagcgc
    21781 atgccttaag aaattcttca aaatacacct gaaaagaatc ggcgatcaca ccatcataat
    21841 cgaaaagaag caacggaatt tctgtgtgca aagagtcagc cattttattc gccctgcatg
    21901 aactcatcca gtcgtgccgc ttccacgatc ctgtactggt atccctgctc agtcaaaaac
    21961 atctgacgct tcaatgcaaa atcctgatca caggtatctt tactgaccag agagtaaaag
    22021 cacgcctgag gggatgccga gactttgggc cgcaggatac gccccagccg ctgcgcttcc
    22081 tcctgccgcg aaccgaaggt gccgctcacc tgaattgcca cattggcatc aggaagatca
    22141 atggcaaaat tagcgacttt ggatacaatg atcagtttga tgtctccttt tcgaaatacg
    22201 gcataaagtt cctgccgtct gcctaccggc gtctgccctg taatgatggg cgccttgaag
    22261 tgccgtgcaa tttctttcaa ctggctcagg tactgaccta taacgagaat attgtcttct
    22321 ttatggcttt caatcagttt ttcgcacagc actatctttt caggattggt ggaagcgatt
    22381 ctgaatttgt tccttttcgg ggccaccgca tattcgaacc gcaggttatc aggcaaaggc
    22441 acacgaattt cggcacaagc cgcttctgca atccagccct gtttttccag aaccttccaa
    22501 ggcacatcat atttttttgg gccgatcaga ctgaacactt cctcttcctg accgtcttca
    22561 cgaaccagcg tggcagtgag ccccagccgg cgctttgcct gaatggaagc cgtcgcccga
    22621 aaaaccggcg ccggaagggt gtgcacttcg tcatagacca cgagccccca cagcgctttg
    22681 tcaaacagat caaagtgtgt gaatggactg ttttttgttt tgcggtaagt cagcacctga
    22741 taggtcgcaa tcgttacagg ccgcacttct tttttgtcgc cgctgtactc cccgatgtca
    22801 tcttcacgca atgtggtctt atccagcagc tcgcttttcc actggcgaag cgctaccgta
    22861 ttggtggcaa ggataagggt atggcttttg actgccgcta tggcgccgat agcgacgatg
    22921 gttttgcctg caccgcaggg cagcacaatc acaccgcttc cgccctgatt ggatccattc
    22981 agatagaagg catccacaga ttctttctga tactggcgca ggccgaaagg ccgtccggaa
    23041 agtgttgttt cccgaaccgc tatttccagc ggcgcaccgt cagcatagcc cgcaagatct
    23101 tcaacaggaa atcctatttt tataagcgcc gacttcagat ttccccggtt ttccgctttg
    23161 acagaaaggg tatgcgtgtc ttctgccgga aaaaccagat ctttaatctg ccgatggctg
    23221 agcaattcac ggataagcag cggatgatct gctgacagca caagaccgcc gtcatccttg
    23281 cgatacagct tgatgcgccc gtaacgggac atctgatcct gaatatccgc aagaatattc
    23341 tgcggtattt catagcggct gaagcgttta agcgaatcaa ccacctcagt ggcagacagg
    23401 cccagagaag cggcattcca tagcgcgagc gggcttaatt tgtatgtgtg gatatgctcc
    23461 ggcgatttaa ccagctcgga aaaagcggcc aggcaatccc ttgcctcttc gaataacggc
    23521 ccgtccgttt cgagcagtat ggatctgtct gactgaacaa taacgggctt ttcttcataa
    23581 cgtgtcatct ggttgtccgg agaggggctg cccctctttc atcagttact gctttcacaa
    23641 caaaaatcga tacagagaaa ggaattcggg aaatcttttg cctgcaggac agaagtgctc
    23701 atcctttcgg gagaaacgct aattcacttc accggaaaac ggatcatact cccagtgctt
    23761 cccggggtaa ggatgcgccg gaatttcatt aagctcccct gcttccacca gcgcctgcag
    23821 ggaagttggg taagcgatgt gcttcgcatg atacctgtcc acagcgttaa tcagtgtatt
    23881 tctggcgcgt ataacctcat cttcaagcag cgtcgtgtta taagattttc cggcagcatc
    23941 cagaaaaaac gtaccgccca gcggatcttc aggcagatac gggatgatgc ctgcctgaag
    24001 aagagtctcc agagaggcgg gataacggcc cttttcctga taaaaccgct gcgcctgctt
    24061 atccagaaca ccgcaaattt tgcgcagttc tatctcgatc aattttctct gtcccagttc
    24121 gcttaaaaaa ctgtccgggc tggcagccat ttcctgccac atgccctgct ccacttcgag
    24181 cagattaaat tcgtcctgta atttcgccgt taagtttaca atcccgcccg gcgccctgcc
    24241 tgttgccgtc gccagcgtta acagcttcgt agcaagatat ttggaattcg gctcttcacc
    24301 tttgttcagc aggtagacca ttgccgcttc atacggaagc tgccaggcat gaggattaat
    24361 ggaaattccg gcataggcaa gcgacaggct ggcttcaggg tcggcacgta aagcagcgag
    24421 aaacatggcg ccgaagcgat agacggcaac gtagtatgga tccagccgtg tcgccgtagt
    24481 cagcatatgt tccatccatt tgaaaacccg cttgcccctg tgttcatgag ctgtatattg
    24541 gatgcagtga agccagataa agtcggcgat aacacaattc agacccgctg tgaaataggt
    24601 gagcaatttt tcattgggca gataaaggac ttcatccgtt gccagggcgt gcctctgcgt
    24661 atcagccaga cgaagaaagg aaactgccag accgaagaag agagaaagcg ccagaaaaac
    24721 gctgataaca cgtacagatc gggtcataca tcacgcctct caaaagccag gcaggcaagg
    24781 atcagcagaa aaagagtcca gctgatgcca tatgccacag catagaaaac atacggccac
    24841 gtgacaggga ccatatttgc cgcttcagac tgaacattaa acagatttaa attcgggatt
    24901 acatagtaaa gtgtttccag aacctgcttt gcccgggtgc cgtcaaactg gggaggcaga
    24961 tcgcgcagca catctgtcgc atgcccgccc acatagaaag aaaaaacgat cagcgccccc
    25021 aaaatcgggg acacaacagt cgacagaaga attgcaaaag ccgtaatcag gagcagtttt
    25081 atgtaaatta aaaggattgc ctgaaaccag atcagcccga ttgcagcacc cgcaatcttc
    25141 aggtaaaggg tcgagcctgc agccataata attaaggata cgcccagtag agcgacaagt
    25201 cccaggtact tgccggttac aaattcatag cgcttcatag gctgagaaag gatggtatac
    25261 agggttttct tatccatttc cttgtacaca agcgttgcac ccacaaaaat ggcaataagt
    25321 gcaccgaaaa cagacattgc cgccagagaa atgtctttga taatctttat atcctgaccg
    25381 atgctgatcc atccgagcac tttggaaccg agtatggtcg ccagtgcaaa gaaaaggagc
    25441 acataaagga ctttgtcgcg gatggattcc ctgaaggtat tctgtgcgat ggttacaatt
    25501 ctattcatga tctgctccat cctttccgga aggattttgc tcccgctgct gtaattttgt
    25561 gaaaaagtct tccagagact ccagaaccgg aatcagcgac aacagcatgc cgcctttttg
    25621 ttgtatctct gctataagag gcggtgcttc cgccacttca ggaagaatga aggtaacctg
    25681 atgttcttca cctcttctgc attcaatgcc tctggctgta atggcgttga catcctcatc
    25741 tttcaagccc gaacagcaga gttcaacagc cttctgcgcc cccgctgtaa ggtctttgat
    25801 cctgcctgaa gctgccaata ccccggaaac cagaatgcct acccggtcag aaatttcttc
    25861 aacatctgtc agaatatgag aactgaagaa aacagttttc ccttccgccc ggagacgcag
    25921 cataatctcc cgcaccattc gcctgccata agggtcaaga ccgctcatag gctcatccag
    25981 cagcaggagc ggcgggttgc cgatcagcgc cacgccaagc cccacacgct gccgcatgcc
    26041 ttttgaaaag ccacggatgc gcctgtcggc aaactcatcc aaaaaaagca tctccgaaat
    26101 acgcagccat tctttccgga tcgcatcttt gctcaatccc cggagtttgc cgtaaaacgc
    26161 aatggtttcg cgcggtgtaa ggtattcgta aaaataagga tgctccggaa gatatcctat
    26221 acgggcacgg gcggacggca aaacggcatc ttcgccaaat atttccgctc tgcctttggt
    26281 cgcctgaatc aatcctgtca gcagtttaat cgccgtggtc ttgccggcac cgttcctgcc
    26341 gagaaaaccg tagatctcat tctcctgcac ttccatattc acattacgga gagaatggac
    26401 ggtggctttt ccgaggcttt tataagtttt gctcaagtca tgggtcacaa tggcggacat
    26461 actgttctat cactcctata ctcagctgtt ctgatccggc gccgtattgg ttttcggtgg
    26521 cttcgctttc cgtccgcgtt ttttcggtgc aaggctccgg ttgtatttct gctcaagttc
    26581 ctgaaaaaat gcggctcttc cgaaggggcg cccggtgcgg gtgcgcatct gcagctcctc
    26641 ttcgagaaga tccgcttgcg gattggtatg aagaaatgca ctccagtggc tcctcatttc
    26701 aggcgatgga aaatgagcgg tcaaaaaggg atgctcattt tctgtagtgt gggcggcggc
    26761 gcttgaccat cgataatcct ccgcacgttc cgccagcctc tgccgacagg gaaggaattc
    26821 cacaatgcgt gcggcaaatt ccacaagcgg atcatcaaga acgcagcttt gaaatcggcc
    26881 acgccagatt gtgccgctgt gaccattcaa cgcattgtaa taccccgaat aggttgcatg
    26941 aatatcccga atcatattgc tcaatgcccg ggcattgggt ggcaccacaa gcagatgtat
    27001 gctgtccgga agaatgcaat acgcccagat atccacacgc tccagcttcg catgtttcgc
    27061 caggagaccg atatattttt caaaatcctg gtccgctgta aaaatcggtt ttttcaggca
    27121 actgcgctgt actacatggt gaacatatcc gccgagaaca aaacgagcca tacgggccat
    27181 gacacttgtc tccttcatta tttccggaaa tgaaaaccgc tttcagaatc agaccgggca
    27241 gctgtaaata acctcattac ccgcataagg atcaacgatt gtaatgcctg attcgttaaa
    27301 tagcgctgtt accggagcac ctttccctga ttccaggcag gcaaaattac gggctgccac
    27361 ttcacgggca gcatgcacaa tcaaaaccga caggcgtcct tgtttagcat tggggtaata
    27421 gtcttctaca tcttccagcg ttttattcaa aaatttcact tcggctattt tttcaaggca
    27481 cacggcaacg ctgtcaacac cgtagccgca ataagccagg ctgccgtccg ggcgcaggac
    27541 atttcgtgtc atgtgcacat tcgctgttct catgccgtct ttttcagaaa cgaaccgcag
    27601 gcctctgtac tgtgtatcgg attcaaccag accgtgtgtg ccgaatatcc ggctctcctg
    27661 attcacaggc gcttcaaagg aatcaggcag gatccagtta ttatgaaaat caatactcat
    27721 tccgttatca aactgaattt tcacctgaac cgcatcaaaa gcatccatgc cgtattcctg
    27781 gcggagtttc tttttttgac ccaccgcgtg gagagacacc ggcttcacat ccatcatgga
    27841 tataaaaaga tcggtccaat gacagccgac ataactgaaa ggatcgctct tttcggccca
    27901 cgctttaaac acttccgttg aaacagcaag aggctcctcg aggagagcca cgccgtacag
    27961 gggctcaccg attttatgca atacatcatc ccgtatgcgg agatgatcgg ggtcatagcg
    28021 cttatgcata tccacgccta caacacgccc tgcccgtttt gacgctgcca gaattgcatc
    28081 ggcttctgcc gcatccaacg ccatgggctt ttccatgatt acatgtgcgc ctgcctcgag
    28141 ggctgcaagc gccggagccg tatgcagatg atccggcgtg gcgatggcaa ggacatccag
    28201 atcaggaaaa tcccgaagaa tatccaccca gggagtctct ccgtggtaaa tagcgaaatc
    28261 cagacctaag gcccgatatt ctgccagacg cgcctttgcg gaggtttcgg tccgggtacc
    28321 gagcgccaca aaatcgatct ctatatcgcc caatgcccgt gccatggaat cgaggcccaa
    28381 acgaccgagc cagggcagaa atccatattg cgcaaactgg caataggccc gcatgtgaac
    28441 atctccgcca aacatgcctg cgcccacaag agcgatggat aaacgtttct tcaaggatag
    28501 cgcctttcta taaattgcta cggcctactt gccccgtcat ggttcaaaaa caaggactgc
    28561 ggacaaacct gtccctgaat atttatattt tagcacagac gccttaaaaa ggaagtatgc
    28621 gcgacggtcc ggctttttcc agtttctttt tttctgctat gatggtggga gtgaatttct
    28681 ctgatgatcc gcaatttaga gaaggacttt gatacatgac aagcaaactt gtggaagctc
    28741 tacaacttaa actgcagccg gtttctttgc tttggcagga cgagaagcct gagaaggcat
    28801 tggaatttaa agaaggcaaa tggggctgtc tcatgtttca tcttgttgcc gcagcgaagg
    28861 ggaaaactgc tgtcgtttca cgaaccagct acg
//
LOCUS       DAOPWB010000019        28843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1579_length_28843_cov_6.125955, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000019 DAOPWB010000000
VERSION     DAOPWB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 28843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28843
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1579_length_28843_cov_6.125955"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PKN92_03260"
     CDS             <1..1199
                     /locus_tag="PKN92_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNZ47843.1"
                     /translation="TDSIPDLAVGRIPVDTLDQATVVVRKIVRYEKNPVNDADFYRRA
                     VVASQFQCCREGAPKDGTDSRSFVEVSEFSRQVLLTAGKEVTRIYGRTGASTPARYYD
                     GTLLPEALSSAKNFAWNGGANDITNAWNNGTFLIIHRDHGLVSGWGTPSYSSNNILAL
                     TNGERLPVVFSINCATGFFDNETANGAYGTTQNGIYFAENALRQPNGGAVSLIAATRN
                     SPSWENSTLLQGFMDAIWTNALPKFGTSQSQRRLGDILNHGKRYVVSKTGMNVMGETI
                     WENAVRNELYLWHCIGDPTLELWVKNPHVQEVLPNYQFNHQDVAIQNGIEVAGGITIL
                     YGVEGAEITVFEEGPNEKMPIGRGVVKNGVAEINYLQKHATTYPLSAVANFENAPALT
                     LEGRKL"
     gene            complement(1287..1646)
                     /locus_tag="PKN92_03265"
     CDS             complement(1287..1646)
                     /locus_tag="PKN92_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNZ47844.1"
                     /translation="MQGKMIKAAKVSDFENRCVRSVRILGRHVAIIKNPDGSFRAMEG
                     GCKHQNADLTTGKVSNGIVTCSWHGWQYDIESGACVQGGSASLRPYACRVEGDDILIS
                     LQPVDPDASSSSFVPFE"
     gene            complement(1658..4192)
                     /locus_tag="PKN92_03270"
     CDS             complement(1658..4192)
                     /locus_tag="PKN92_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ47845.1"
                     /translation="MAKQLLINPKKVRNRSTLELGSIPINQYSRSVREESTSKGVGME
                     RCLRIYRDMALIREFETMLDSIKKLGAYQGIEYNHAGPAHLSIGQEGAAVGQSVHLSV
                     DDHLFGSHRSHGEFIAKGLRAVEDLADSSLSEIMENYFGGRTLSIVEKNEADWSPFSL
                     TPQKSRKAALQTTASEERGIDFLLYGLLAEVFGRETGFNKGMGGSMHAFFPPFGVYPA
                     NAIVGGSADISVGAALYKKVQEKKGIAIANIGDASIGCGPVWEALGFSAMSQTRTLWP
                     DFMKGGLPMLFNFMNNFYGMGGQPIGETMGFDFLARVGAAVNAENMHAEVVDGNNPLA
                     LADAYARKLKLIKAGKGPVFLDVLCYRQAGHSPSDQSAYREREEIDLWRAVDPITEYG
                     QNLVQAGILAEGDLEAVAEYARRKVTKACRLAVSEEVSPRMFLSSRQGVSQMMYNNQV
                     EEALPGLRRKEDVCMPLEENPRLQAIARKSRSGIAPDGSVLKGTKAVTLGEALFETII
                     HHFYNDHRLVAYGEENRDWGGAFAVYQGLTEALPYYRLFNSPISEAAIVGTAVGYAME
                     GGRPLVELMYCDFMGRAGDEVFNQMPKWQAMSAGLLRLPIVLRVSVGAKYGAQHSQDW
                     TALCAHIPGLKVVFPATPYSAKGLLASALSGNDPVVFFESQRLYNQVELFQEGGVPAE
                     YYRLPIGVPARIKEGKDLTVLTFGATLYRALEAAKRFEQEYNLSVEVIDGRTLVPFDY
                     SLLLESVKKTGKVIIASDACERGSYNHTIAGQLTQLAFDDLDAPVCVIGAPNWIVPPA
                     EMEDAYFPQAFSFLDAYHQQIQPLPGYTPVISRTADEMMYQNRFGI"
     gene            complement(4385..5257)
                     /gene="dapA"
                     /locus_tag="PKN92_03275"
     CDS             complement(4385..5257)
                     /gene="dapA"
                     /locus_tag="PKN92_03275"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNZ47846.1"
                     /translation="MLFSGSYVAMVTPFQESGAVDFDAYGRLVDFHLEQGSDGLVPCG
                     CTGEAATLSHDEQKACIRFVVERVAGRIPVIAGTGSNNTQEALTLTRYARELNCDAAL
                     LITPYYNKPTAAGQILHYTTIAEAVDIPIMLYNVPGRTGVKMEPETVAALSRVPGIQS
                     IKEACGSVDQVSNILDQCSIIVMSGDDSLTLPMMSVGATGVVSVAGNVAPRAVAELCR
                     HANEGAYEEARTIHYRLHALFKALFWETNPMPVKAALAEMGLIKNILRLPLVPMSEKY
                     QDKLRAVLKELDLV"
     gene            complement(5314..6801)
                     /gene="dnaB"
                     /locus_tag="PKN92_03280"
     CDS             complement(5314..6801)
                     /gene="dnaB"
                     /locus_tag="PKN92_03280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNZ47847.1"
                     /translation="MLSESDSRSGAGATLKAHYDRTPPQDVEAERAVLGAILLRPSAI
                     SAVLEILHGGETGVFFIPVHQVIFNALIALFTTGRAIDRVTVMSEIGVNPAFDSAGGE
                     AYLTDLIYAVPTSANVEYYAEIVQEKALRRRLIEMCAQVTSESYRTEQRVEDLLDVAE
                     QQIFSLNQQRHTNKIYSISALLGSTIELIEKNLKAGEHITGLATGFHGLDNLLSGLQP
                     SDMVILAARPSVGKTALALNIAANAATRNDKSVLIFSLEMAKEQLTHRMMCMVGTVDA
                     GRLRKGFLSDHEFPKVQKAAGILAQAKIFIDESAGLTPLDLRSKARRFASQNPLDLII
                     IDYMQLMHTSGRNENRQTEISEISRSIKGLARELSIPILTLSQLSREADKDDGGSPKL
                     SHLRESGAIEQDADVVIILSRPPASDREGKENMILATVAKHRNGPTGRVDLIFQSNYQ
                     RFVDADTGYGAQRAAREDTYSAPADDYYADPIMEAPFSEDDEEIF"
     gene            complement(6803..7333)
                     /gene="rplI"
                     /locus_tag="PKN92_03285"
     CDS             complement(6803..7333)
                     /gene="rplI"
                     /locus_tag="PKN92_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNZ47848.1"
                     /translation="MNVILCEHIEKLGEIGEKVSVRNGYARNYLFPRKLAVPADSASA
                     KQIEHELGILKRREEKRRTLLTAEADKMRGLTLEFKVRAGEEDKIFGSVTTAMIAEKL
                     KEAGFDVDRKKVLLDDPIKALGIYSVEIKVYPGITSAVKVWVTGLEDEVTTSAATVEA
                     TSAADEAEGDASDATA"
     gene            complement(7398..8330)
                     /locus_tag="PKN92_03290"
     CDS             complement(7398..8330)
                     /locus_tag="PKN92_03290"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HNZ47849.1"
                     /translation="MPVYLFLCTLLGALIVLGRSTEGILPVLLFSVYPVGWYWSQGHR
                     FRAVGLPVLMWLGAAVVSGDFLLALVLLFGLLPGILLGTLILQRQTPGRCLAAVTAVL
                     FLFVGGSYALMWPAVSLEWHQAFENYIEQMSQNASDLRSENAIATFRWLDGHWAYVSF
                     GLLFGMILLCQLLLVLLLFSRLQKRAVLKGTRYSAGRLRMPEPLVWVAIVVALGWFWD
                     SYYPNDSLRLVVWNSAIMLAFIYLVNGISITAFALVLFRFQSLWLAFAVLILILFNLY
                     QLLPFIGFFDTWINFRAWLLRMRHRQLKAKDPDL"
     gene            complement(8393..8647)
                     /gene="rpsR"
                     /locus_tag="PKN92_03295"
     CDS             complement(8393..8647)
                     /gene="rpsR"
                     /locus_tag="PKN92_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNZ47850.1"
                     /translation="MKVREKKSRRRKKKKSLRAKVCRLTIDRVVYIDYKDVNLLKHYV
                     TERGKIIPRRITGATAKHQRMLNRAIKLARQAALLPYLAD"
     gene            complement(8695..9159)
                     /gene="ssb"
                     /locus_tag="PKN92_03300"
     CDS             complement(8695..9159)
                     /gene="ssb"
                     /locus_tag="PKN92_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNZ47851.1"
                     /translation="MGDVRVPDLNHVFLAGRLTRDPELRYLQNSNKAYCRFSVANARY
                     YKDKTTGERREETTFVDITAWGPQGEFVGEHLKKGRPVLVEGRLHTSEWEDQASGQRR
                     TRLAVTAVRVTPLDWEDSGGGRGSYQSAQSESRAEQRPREIEEPVPEDDIPF"
     gene            complement(9227..9685)
                     /gene="rpsF"
                     /locus_tag="PKN92_03305"
     CDS             complement(9227..9685)
                     /gene="rpsF"
                     /locus_tag="PKN92_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNZ47852.1"
                     /translation="MRTYEALYIINPELDDSAIQTITTDVEQLITNNGGSIVRSDVWG
                     RRKLAYTIQKHTEGVYVVLRFTADPAFVHKLEQYFKLSETVIRSMVLYLDAKTLKLEE
                     QQQRQYEEDLRQSAARRSHSDDDDDDDNGDDEDGGYRHRGRDSRDDDDDD"
     gene            10040..10810
                     /locus_tag="PKN92_03310"
     CDS             10040..10810
                     /locus_tag="PKN92_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47853.1"
                     /translation="MSDDQLDGTEPREEGVSGSAVFGTARGLIQRMRECSQEAYIKSD
                     EQVVYLWACVTRTGFCNHSKDTDHPIAPLTLEDWLNVVDESATLGANWLILTVGDPLH
                     LCPDVWELSRWAQESYGMTVGLHPKNGGLCAEDLEAIKQLDINNTRLLLREEWTDARK
                     LAENEGIIVWTANPQPPGERPHCKGGTRMIFVNEHGILYTCGVVEGNSNYHLGHVLDK
                     RLKQVVTDPKRPRWVEKSLHVVSENCDGCPARMAVFFS"
     gene            10883..11521
                     /gene="lexA"
                     /locus_tag="PKN92_03315"
     CDS             10883..11521
                     /gene="lexA"
                     /locus_tag="PKN92_03315"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNZ47854.1"
                     /translation="MAQEITPRQAAVLNFIAESIEKLGFPPTIQEIGQRFGIVSTNGV
                     FDHLKTLERKGFIERSSKARSIRLTPKAAAGLVRRHADALPLIGRVAAGYPLLAMENI
                     EEYIPVAPRLARKNAFCLRVTGDSMIDAHILEGDIIVVDRDRRPATKDIVVALVEEEA
                     TVKYYYPQNGFVELRPANAALQPMIFPAEEVSVQGVVISLQRDLGTASAPAF"
     gene            11619..13880
                     /locus_tag="PKN92_03320"
     CDS             11619..13880
                     /locus_tag="PKN92_03320"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNZ47855.1"
                     /translation="MIFDLAMIQRFYKSLPELVAAARKALRRPMTFTEKILYAHLSVG
                     LPEQPFMRGKDYVDFDPDRVAMQDATAQMALLQFMQAGKEKSAVPTTVHCDHLILAEN
                     GAGPDLLRALQMNEEVFNFLASVSARYGIGFWKPGAGIIHQIVLENYAFPGGMMIGTD
                     SHTPNAGGLGMVAIGVGGADAVDVMAGMPWELKFPKIIGVRLTGTLKGWTSPKDIILK
                     VAGELTVKGGTGAIIEYFGEGTASISCTGKGTVCNMGAEIGATTSVFPFDESMAAYLE
                     ATGRREVAEAARNAASCLRPDPEVLQAPESYYDRIIEIDLDTLHPHLNGPFTPDLATP
                     VPEMKKAAQEGGWPLQVEVGLIGSCTNSSYEDMSRAASIARQAKEKGLTAKAQFTITP
                     GSELIRSTLERDGILDDFTAIGGVILANACGPCIGQWKRHRNGADDEPNTIVTSFNRN
                     FAKRNDGNPKTHAFVASPEMVTALALSGRLDFDPATDTLVNNAGEAVRLTPPQGDALP
                     ARGFADGDSGFVAPPEDGSAIKVLVKPDSERLHLLEPFSPNHGDICGMKLLIKAKGKC
                     TTDHISMAGPWLRFRGHLDNISNNCLIGATNAFNDKNNTVKNQLTGAYEEVPATARAY
                     KAAGIPSLVVGDENYGEGSSREHAAMEPRHLGVRAVLVRSFARIHETNLKKQGMLALT
                     FSDPADYEKIREDDTICITGLDRFTPGVPLTVTLKHADGSEESFPVNHSYNETQIAWY
                     QAGSALNLIRTQH"
     gene            complement(13887..15041)
                     /locus_tag="PKN92_03325"
     CDS             complement(13887..15041)
                     /locus_tag="PKN92_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNZ47856.1"
                     /translation="MRSVFVFGVCCLATLTSFAAPTFVAHKIGSYRGEVLNVADFNND
                     GLLDIVALPWIYLAPDFKGYEICTIEGEVDEEGKGYRWDFVNAPVDVDGDGLTDVVTA
                     SWFGMKAEWLRNPGTFDTQWERHLIVENGNYECGDLCDLDGDGKLNQVLPAVLKTVWY
                     EPGMKDGKQQMLRYVISEKEMEFGVGYGDINGDGRPDVLRPSAWFEAPENPRADPWIE
                     HPLALGGKEEGTSGHTAPILVYDVNNDGLADIISAIAHDYGIFWWEQQRDGDAITWKR
                     HLIDDSWSQPHSLALADLDGDGDLDLVAGKRFMAHNGGDPGAFEPLCVYWYELERAPA
                     PAWKRHVISYDEGIGSGLNMIATDLDGDGDADIIVTGKWGGPVWFENKLK"
     gene            complement(15214..15807)
                     /locus_tag="PKN92_03330"
     CDS             complement(15214..15807)
                     /locus_tag="PKN92_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HNZ47857.1"
                     /translation="MGRFRRSFSRTYGQRSYKKLFVIATEGKKTEPAYFRMLADSSKL
                     VKIKCIKGDAKSAPGYVLKRMVLCLQEEDLQKGDEAWIVIDQDQWEEHQMETLFVWEK
                     ATENRGIALSNPNFEYWRLLHFEEGTKLAHAEDCSRRLKKYIPGYDKGIDPGHFTSEQ
                     VRQAVERARARTKGKPAACFGSTSVYRLVENILTARD"
     gene            complement(15807..17141)
                     /locus_tag="PKN92_03335"
     CDS             complement(15807..17141)
                     /locus_tag="PKN92_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ47858.1"
                     /translation="MLTRFTINNWLSFADTAELSMLATPERQHSERVARVKPYAKVLP
                     VSVVYGGNASGKTNFFKALHFVQQFVVQGRRPDSPIPVTPFLLDQLKRQQPSRFTVEL
                     FAEGAFYEYGFSVTCTEVLEEHLVRIGNKTEKLLFSREQGRYEFDRALPEQKYLDFVA
                     RGTRQNQLFLTNSVFQNLKHFQAVYEWFKSHLEMIGPDGRFAAFEQRFDQTKIFSDQM
                     NRLLQHLDTGIRRLETEAVAFEDLPLPAPVQAMLHEELLEGKTLRVDGPMNSRFLLSR
                     KAGALKAEKLFGYHAAAGGEEVRFELSQESDGSQRLIDLLPAFVDLLAEASSKIFVID
                     EVDRSLHSLLTQKLLDLYLASCTALTRKQLILTTHDLGLMDQRLLRRDEMWVTERNDR
                     GESSLVSFSEFKDVRYDKDIRKSYLQGRLGGVPVLARVMEDPAVYTTGASED"
     gene            complement(17228..17833)
                     /locus_tag="PKN92_03340"
     CDS             complement(17228..17833)
                     /locus_tag="PKN92_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNZ47859.1"
                     /translation="MIERIDHVAVAVKDHDAALQFFRDVLGAVPGEKFKASDRNFYWQ
                     TLALGDLSRLELISPSGPDSFLDGFLAKKDGGVHHITLQTPNLEAMLKKLEAHGVPYF
                     GKHAYPDGSWKEVFVHPKDAFGVLVQISEFDAEYWMVPDAKMPEGVRFQVASEEKGVT
                     LTFAHPGGGKVCVECSREEVKALAEQIEKALAGKNIKKADE"
     gene            complement(17863..20361)
                     /locus_tag="PKN92_03345"
     CDS             complement(17863..20361)
                     /locus_tag="PKN92_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNZ47860.1"
                     /translation="MRTPVLVQKRAWFFLLTVLLSLHASGSLEDTVWQRAAENGEIAR
                     EALVKSLRFVHAWLQTADPETGLIPRNLKDSPYWNAKDSAADNYPFMVLTTYFTDRTL
                     FDGRMKTMLETEQRLCNRLGRLPDDWLFEPQGFRVQEVRSDDLIFGASEYMKDGLIPV
                     TELLGPSPWSERMLGMLEDLWAYGAVETEIGRLPSTSHEVAGNLLQLCSRIYWMTGEE
                     IHRRHVFQLGDYFFLHHLPTETERLQLDDHGCEVINGLSEAYFVAAKTDPEKHAQWRK
                     PMHAMLDRILETARDENGLLYDLINPKTGEIKSRELTDNWGYNYNAFAVVAEVDGEER
                     YAEAVRHVLSNLPAVKDYRWEYGSADGYADSLEGGLNLLNRYPVAEAAEWADYTARIL
                     LDKPRDTGIVEGWHGDGNFARTALMYAFWKSQGAWLHPWRNDLRLGAVSPEPGTWCFH
                     IASDWHWQGAVNFDLPRHAVYLHMPEDYPRLNQFPEWFVIREDQQYALQVDDNPVLYL
                     RGKDLSSLPLRLTGDKPRRIILRENAAAPAAPPVPTESVQAFTEWQQETRKALFEVLR
                     ITDLTEGSGLPLEAAPEVRTEKDGFVLCEVEIQGLPGYRLPAVLGLPAGEGPFPAVVC
                     IHGHGDTRYSVFEEKPESAYKGIGARLAKAGYVTMAVDVGGHEALEVSRELMGERLWN
                     LMRCVDYLTSLKVVDPKRIGCAGLSLGGEMSLWLAATDTRIRAAVSGGFLTLMDQMEQ
                     NHCMCWKFPGLRSLVDYPGLASLAAPRSLQFQNGMKEPDNQFPPWLARLAFRQIQPAY
                     ACLDASDRLFLHVHPGGHELDYYGLLRFFDTHLK"
     gene            complement(20455..21918)
                     /locus_tag="PKN92_03350"
     CDS             complement(20455..21918)
                     /locus_tag="PKN92_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNZ47861.1"
                     /translation="MKAPHIILFLFTALILSGVSAEAQASDSLKLSDPVAEKVASGQT
                     TLSDQTDVAITIYNNDRALIRDRRKIRLLPGEAALRFMDVASTILPETVSLRSLNDPG
                     ALKILEQNYEYDLMSPDKLMEKYVGRNVTLINKNTDYSFYEQPAKLLSVNNGPVYEVD
                     GKIYLGHPGVVVLPEIPEALIAKPTLVWLLSNDATDHEVEASYLAGGISWRADYVLTV
                     GKDEKTMDLEGWVTLDNQSGATYNNAQLKLVAGDVNIAAPAPVMYREAKAMDMMMAGA
                     PPMMEESFSEYHLYALQRRTTIKEKQTKQVSLLSGKGIKISKKYEFRGNLAFYSSRLD
                     AQKNQNADAFIVFENKEENGLGIPLPAGIMRVYQPDSSGMLQFSGEDRISHTPKNEEV
                     RLILGKAFDVVGDRTQKDYKNLGPNTHEVEFELSIRNRKDTAIQLDVVEPMPGGDWQI
                     LKQSHAFVKKDARTAVFTVDIPADSEVTVNYRVRVSY"
     gene            22128..23339
                     /locus_tag="PKN92_03355"
     CDS             22128..23339
                     /locus_tag="PKN92_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ47862.1"
                     /translation="MKLTGKVLVAQGGGPTAVINQSVVGVVLEARKYPEVTRIYGARH
                     GVNGIINENFLDLTDVPTANLEAFAASPSSGLYSTRDKPDAAYCKKIFEICKAHDIRY
                     FFYCGGNDSADSCNTVSKEASNAGYEMHVIHVPKTIDNDLKVTDHCPGYGSAAKFVAQ
                     AFAGADLDNRALPGVYIGVVMGRHAGFLTAAAALGRKYEDDGPHLIYLPERTFNLERF
                     KNDVENVYAKYGRCVIAVSEGIVDETGKPIAAKFTGGEKDAHGNVQLSGSGALGDHLA
                     KYIKDEAKISRVRADTLGYLQRSFLGCVSEVDQVEARTVGEMGVRYAIGQQKSGSVAM
                     KRAGNYSISYFLTALDTVARDSIEMPDEYINAAGNDVTEAFIKYARPLVGTLTEATLI
                     DAPAVQKILKK"
     gene            23477..24445
                     /locus_tag="PKN92_03360"
     CDS             23477..24445
                     /locus_tag="PKN92_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNZ47863.1"
                     /translation="MKKTLLLSLVMVAGIIVTGCGKQAAAPGETADKAPDKLRIALVM
                     KSLANEFFLTMEQGARAHQAAHADQYVLLADGIKDELDVNRQIQLVEQMMARHVDAIV
                     IAPADSKALVQVCQRAQEAGIIVINIDNKFDDEIMKQRGVHIPFVGPDNRKGARLAGE
                     YLAARLQPGDPVAIIEGVPSAFNGIERRLGFEAAMKEAGMNIVASQVGDWEMDKAHRV
                     VSALITEHPDIKAFLCANDSMALGAVAALRSAGKLQGIPVIGFDAISAAVDLVKKGEL
                     LATVTQHADRLAVFGIEAALEVLKTKATPEDMETPVELVTAEALAS"
     gene            24442..25386
                     /locus_tag="PKN92_03365"
     CDS             24442..25386
                     /locus_tag="PKN92_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ47864.1"
                     /translation="MIQGRQLLRALVNYAGLALVLGLLVTLFALSTDYFFTWTTFRTI
                     ANQIPDAVILATGMTFVLIIGGIDLSVGSVLALSGAVLGLAMVRWQLPLPVAVLLCLG
                     TGALCGVLNGLFVIRWQLPAFIVTLGMLEAARGGAYLVTGSKTQYIGAGMDWISRWSL
                     LGLSLPFLVAMITVITGQLVLTYTPFGRSLYAIGGNEEAARLSGIPIGRAKVAVYALC
                     STLSALAAVIYCSRLTAADPNAGSGYELQAIAAVVIGGTSLMGGRGSVINTLFGVIII
                     AVLESGLIQMGAQEPVKRLVTGGIIVAAVVIDYYRGKI"
     gene            25484..27664
                     /locus_tag="PKN92_03370"
     CDS             25484..27664
                     /locus_tag="PKN92_03370"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HNZ47865.1"
                     /translation="MRYFLPLTLLILCGSVSADDAGSFVFLDDGDNTGVLYTYASTEI
                     QDYFAKMFTTKLVTEVPEGTAPDVVIRLGTPDSNTAIADAVAAGQLVLPEGALSDQGY
                     TLKNLENQLFIAAPAPQGVLYGTYAFLEAYGAYFQISGERLPAPLPFTLKTLDLTEKP
                     AVKFRGLLPWDAYICGIAGYNPEDYRQLIDRAARLKLNFLQFHFYPGSAFFTETIDGT
                     TAEPRYLGMPVDIFPTANAVGKAAFGDVIAFGAQCYIDHAGSPAAQAHAVQDMMRDAL
                     AHAKNRGMHTAVGMELMQPSVGSPLFTAKPEGSVNCLDPLRPENLALSRQRYLSLKET
                     YPDADFYCFWQSEGQGVRGAAVGQEEGAAAMREAQAHWTSQDNYRGDIDYAWLLKQLC
                     DSMTPEEQATSVAGGWFLQHLFPGIHPDFPAGIRFSSINNYSMEQSLDGGLDYYAVAA
                     ENRAVWVTDWWEYSGEQWFPQWRAGGQERIYRQCLDYGIEALSLVGWKLSGIEHNIRF
                     MADFAWHPGLTAEQFYRDYVSRLYGQDSAPLADLYMTADAKSELTPPATSSDDRPMSL
                     GEGWIVLGIPPFPDTAEGLSTEEWRHTAERAAAIAAEQQTFLQSDRDAVALIGQIIPG
                     LDSQGQYWAALLLNRLRMRLHYLESMIALNESYGIYQAAAPQGIPAARAAAALRLNDA
                     VALAGEAIEIYSKEIRNRNDLGVVAQMNVQYLQPLVQLHASLSD"
     gene            27698..28699
                     /locus_tag="PKN92_03375"
     CDS             27698..28699
                     /locus_tag="PKN92_03375"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ47866.1"
                     /translation="MHRFVILALTFLLTASFSYADDDDGIMGNYQGAFTSPGAAGKTV
                     RAQIVAYGKGNYTLVLFLGDEKGEFSRDEIRSHVKKGQLSFSKSIRVAPWGGNFTLTA
                     QAAEETLAGTLSPGKKTSAESLSFELKRVFLEPPTRGMAPPEGARVLFDGNNLDQWER
                     HPRVWNIQDDGGMQVCGSSLKTLENFGSGLYHVEFMTPFTPEKRGQARGNSGVYLLGI
                     YEIQVLDTFGLPPADNHCGGLYSLATPGTDALLPPLQWQTYDITFEAAEFDDAGNKTK
                     NARLHVLHNGIVIHDNIELPGPTPGGISDQESASGPLLLQDHRDPVRFRNIWFKPAS"
BASE COUNT         6834 a         8251 c         7204 g         6554 t
ORIGIN      
        1 aaaccgattc cattcccgat ctggctgtcg gccgtatccc cgtggacaca ctggatcagg
       61 caacagtcgt tgtccgcaaa atcgtccgat atgaaaagaa tcccgtaaac gatgcggatt
      121 tttaccggcg cgctgtggtg gcatcccagt ttcaatgctg ccgcgaggga gcaccgaaag
      181 acggtacgga cagccgttct tttgtcgaag tatccgaatt ttcccggcag gttcttctca
      241 cagcaggcaa agaggtcaca cgtatttacg gacgcacagg tgcttctacg cctgcccgct
      301 attatgacgg aaccctgctt cccgaagcgc tctcttctgc caaaaacttt gcgtggaacg
      361 gaggagccaa cgatatcacc aatgcctgga acaacggtac tttcctgatc attcatcggg
      421 atcatggtct ggtaagcggg tggggtacgc cctcctacag cagtaacaat atactcgccc
      481 tgactaatgg agagcggctg cctgttgtct tcagcatcaa ttgcgcaacc ggtttctttg
      541 ataatgagac cgccaacggc gcctatggca caactcagaa cggtatttac ttcgccgaaa
      601 acgcattacg ccagccgaac ggtggcgccg tttcattgat tgccgccaca cggaacagtc
      661 ccagctggga aaactccacg ctccttcagg gcttcatgga tgccatctgg accaatgcgc
      721 ttcccaaatt cggtacaagt cagtctcaga gacggctggg cgacatcctg aaccacggca
      781 aacgctatgt tgtgtccaaa accggcatga atgtcatggg cgaaaccatc tgggaaaatg
      841 ccgtgcgcaa cgaactgtac ctgtggcact gcatcgggga tccgacgctt gagctgtggg
      901 tgaaaaaccc ccatgtacag gaagtgctgc cgaactatca gttcaaccac caggatgtgg
      961 cgatacagaa cggcattgaa gttgccggag gcattacaat tctgtacggc gttgaaggtg
     1021 ctgaaatcac cgtcttcgaa gaaggaccga atgagaaaat gcccatcggt cgcggtgtgg
     1081 tgaaaaacgg cgtcgcggag atcaattatc tccagaaaca cgcaacgacc tatcccctct
     1141 ccgcggtggc aaattttgaa aatgcgcctg ccttaacgct ggaaggcaga aaactctaac
     1201 cctgtcaatg catattcccc ttcctgtgcg gggaggcgcc ccggcggcgt ctcccccttt
     1261 ttttgtcgcc tgacccggaa aaaagatcat tcaaagggga caaaagaaga ggaagaggcg
     1321 tccggatcaa cgggctgcaa tgaaatcagg atgtcgtcgc cttccacccg gcaggcatag
     1381 gggcgcaaag acgcacttcc gccctgaaca caggcgccac tttctatatc gtactgccag
     1441 ccatgccagg aacaggtaac aatgccattg gaaacctttc cggttgtcag atccgcattc
     1501 tgatgcttgc agcccccttc catggcacgg aagctgccat caggattttt aataatggct
     1561 acatgcctgc cgagaatgcg gacacttctt acacagcgat tttcaaaatc gctgaccttc
     1621 gccgctttga tcatcttgcc ctgcatgaaa aattccgtta aatgccgaag cggttctgat
     1681 acatcatttc atccgcagtg cgggaaatta ccggtgtata gccgggcagc ggctggatct
     1741 gctggtgata ggcatcgagg aaagagaatg cctgcgggaa gtaggcgtct tccatttccg
     1801 ccgggggcac aatccagttg ggtgcgccaa tcacgcaaac cggtgcgtca agatcatcaa
     1861 aggcaagctg cgtcagctgc ccggcaatcg tatgattgta gctgccccgt tcgcaggcat
     1921 cgctggcaat aatcactttg ccggtctttt tcaccgattc aagaagcagg ctgtaatcaa
     1981 agggtaccag cgttcttccg tcaatgactt ccacggacag attgtattcc tgctcaaaac
     2041 gtttggcagc ttcaagcgcc cggtaaaggg tcgcaccgaa agtaagaacc gtcaggtctt
     2101 tgccttcttt tattctggca ggcacgccta taggcagccg gtagtattcc gcgggaacgc
     2161 cgccctcctg aaacagttcc acctgattat agaggcgctg gctttcaaag aaaaccacgg
     2221 gatcattgcc gctcaaagcc gaggcaagga gccctttggc gctgtagggc gtggcgggaa
     2281 agaccacctt caatcccgga atgtgcgcgc aaagtgctgt ccagtcctga gaatgctggg
     2341 caccgtactt ggcgccgaca gacacgcgca gcacgatggg cagacgcagc agccccgcag
     2401 acatggcctg ccatttaggc atctggttga aaacttcatc ccctgccctg cccataaagt
     2461 cacagtacat gagctcgacc agagggcgcc cgccttccat ggcatagccg actgccgtac
     2521 ccacaatggc ggcttcggaa atgggcgagt tgaaaagacg gtaataagga agcgcttcgg
     2581 tcagaccctg atatacggca aaggctccgc cccagtcacg gttttcttcg ccataggcaa
     2641 ccagtctgtg gtcattgtaa aaatgatgga ttatcgtttc gaaaagcgcc tcgcccagag
     2701 tcaccgcttt ggtgcctttg agcacggaac cgtcgggagc aataccggaa cgggacttgc
     2761 gcgcaatagc ctgcagcctc gggttctcct caagcggcat acacacatct tctttgcggc
     2821 gcagtcccgg cagcgcttct tccacctgat tgttgtacat catctgggac acgccctgcc
     2881 gcgatgacag gaacatgcgt ggcgacactt cttcagaaac ggcgagacgg cacgccttgg
     2941 tcactttgcg tcgggcatac tcagccacag cttccagatc cccttctgca agaattccgg
     3001 cctgcaccag attctgtccg tattccgtaa tgggatccac ggcgcgccac aaatcaattt
     3061 cttcccgctc cctgtatgcc gactgatcgc tcggagaatg gcccgcctga cgatagcaaa
     3121 gcacatcgag gaatacgggt cctttccctg ctttaatcag tttcagctta cgggcataag
     3181 cgtccgccag tgccagcgga ttattgccgt ccacgacctc ggcgtgcatg ttttccgcat
     3241 tcaccgccgc accgacacgg gcaaggaagt caaagcccat ggtttcgccg atgggctgcc
     3301 cgcccatacc gtaaaaattg ttcatgaagt tgaagagcat gggaagtccg cccttcataa
     3361 aatccggcca gagtgttcgg gtctgggaca tggcggaaaa acccagtgcc tcccagaccg
     3421 gaccgcagcc gatggatgca tcgccgatat tggcaatggc gattcctttc ttttcctgca
     3481 cctttttgta caacgccgcg ccgacactga tatcggcgct cccgcctaca atggcattgg
     3541 cgggataaac gccgaaaggc gggaaaaagg cgtgcatgga tccgcccatg cctttgttaa
     3601 atcccgtttc tctgccgaaa acttccgcaa gcagtccgta cagaagaaaa tcgatacccc
     3661 gttcttccga tgctgtggtc tgcagtgccg ctttgcggga cttctgaggc gtcagcgaga
     3721 aaggcgacca gtcggcttca tttttttcga caatagacag tgtcctgccg ccgaaatagt
     3781 tttccataat ctctgaaagc gaagaatccg caagatcttc aacagcacgc agtcctttcg
     3841 cgatgaactc cccgtggctg cggtggctgc cgaaaagatg atcgtcaacg ctcagatgca
     3901 cgctctgccc taccgctgcc ccttcctgac cgatagacag atgtgcggga cccgcatggt
     3961 tatattcaat gccctgataa gcacccagtt ttttgatgct gtccagcatg gtctcaaatt
     4021 cacggataag tgccatgtcg cggtaaatgc gcaggcagcg ctccatgccg acccctttgg
     4081 aggtgctttc ctcccggaca ctgcggctgt actggttaat cggaatagat ccgagttcga
     4141 gcgtgcttct gttgcgtact tttttcggat tgatcaggag ttgctttgcc atgaaaaatt
     4201 accttcgcgg aacgaacccg cctgttgggc cgcagggtta cggtgaaatg tgaccaaaaa
     4261 tcccgtttag tgtaccagaa aaaagaggac atgcgccaag agccgcagca agacattgtt
     4321 cagagcaaac tggtttccgg tcaggcgttt ttttgaaaac cgggcggaac cgcgaaaccg
     4381 tcactcagac gaggtcgagt tctttcagca cagcacgcag tttatcctgg tatttttctg
     4441 acatgggaac aagcggcagg cgcaggatat tctttatcag tcccatctcc gccaatgctg
     4501 ctttcaccgg catgggattg gtctcccaga aaagcgcctt gaaaagagcg tgcagccggt
     4561 aatgtattgt ccgtgcctcc tcatacgccc cttcattggc gtggcggcac aactccgcaa
     4621 ccgcacgggg cgcaacatta cccgccacgg acacaacgcc ggtcgctccg accgacatca
     4681 tgggcagcgt caggctgtca tcacccgaca tgactataat ggagcactga tccagaatat
     4741 tcgatacctg atccacactg ccacaggcct ctttaatcga ctgaataccg ggtacccggg
     4801 acagagcggc aacagtctcg ggctccattt ttacgcctgt ccgccccggc acattgtaaa
     4861 gcataatcgg gatatcgacc gcttcagcga tagttgtgta gtgcagaatc tgcccggctg
     4921 ctgtcggctt attgtaataa ggggtgatca gaagcgccgc atcgcagttc agttcacggg
     4981 catagcgggt cagcgtgagc gcctcctgag tattgttgga gcctgtaccc gcaatgaccg
     5041 gaatgcggcc tgccacccgc tcgaccacga agcggataca cgctttctgc tcgtcgtgcg
     5101 acagcgtagc ggcctcgccg gtacagccgc aggggacgag cccgtcgctg ccctgctcaa
     5161 gatggaaatc gaccagtcgc ccgtaggcat caaagtcaac agcaccggac tcctgaaaag
     5221 gagtgaccat ggcaacataa gatcctgaaa aaagcataac agtctccaaa gccgcatcaa
     5281 gggcagttac tcagaatgaa ttctggggat cttctaaaaa atttcttcgt cgtcttcaga
     5341 aaagggcgct tccatgattg gatcagcgta atagtcgtct gcaggggcgg aataggtatc
     5401 ttcccgcgcc gcccgttgcg ctccatagcc cgtatcggca tctacaaagc gctgataatt
     5461 cgattggaaa atcaggtcaa cgcgacccgt cggtccgttt ctgtgctttg ccacagtcgc
     5521 taaaatcata ttttccttgc cctcacggtc agatgcaggc ggacgggaca gaataatcac
     5581 cacgtccgca tcctgctcga tggcgcccga ttccctcagg tgggaaagct tgggactgcc
     5641 gccgtcatct ttatccgctt cccggcttaa ttgcgacaag gtcaggatcg ggatggaaag
     5701 ctcccgggcg agccctttga tggatctgga aatttccgaa atctccgtct ggcggttttc
     5761 attccgtccg gacgtgtgca taagctgcat atagtcgatg ataatcagat ccagaggatt
     5821 ttgagacgca aaccgacgcg ccttcgagcg caagtccaga ggtgtaagcc cggcggattc
     5881 atcgatgaat atcttcgcct gagccagaat gcctgccgct ttctgcacct ttggaaattc
     5941 atgatccgag agaaagccct tgcgcaggcg gcccgcatcc actgtgccaa ccatacacat
     6001 catacggtgc gtcagttgtt cttttgccat ttccaggctg aaaatcaaca cagacttatc
     6061 attgcgtgtc gccgcattgg ccgcaatatt caaggccaac gccgtttttc ccacagaagg
     6121 cctggccgcc agaatcacca tatccgacgg ctgcagtccg gacagcagat tatcaagccc
     6181 gtgaaaacct gtggcgagac cggtaatgtg ctcgcctgcc ttcagatttt tttcgatcag
     6241 ttctatggta gatcccaaca gggcggatat ggaatatatc ttgttggtgt gccgctgctg
     6301 gttcagcgaa aaaatctgct gttccgccac atccagcaaa tcctccaccc gttgttcggt
     6361 gcggtaggac tcagaggtga cctgagcaca catctcaatc agacggcggc gaagtgcttt
     6421 ttcctgaacg atttccgcat aatattccac attggcagaa gtgggaaccg cataaatcaa
     6481 atcggtcaga taggcttcac cgcctgcaga atcaaaggcc gggttcacgc cgatttcact
     6541 catcaccgtg acccgatcaa tcgccctgcc tgttgtgaac agggcaatca atgcattaaa
     6601 aataacctgg tggacaggaa tgaaaaaaac acccgtttca ccgccatgca ggatttccag
     6661 aacggcgctg atcgctgacg ggcggagcag aatcgctccc agaacggcac gctcagcttc
     6721 tacatcctgt ggcggcgtgc ggtcataatg tgctttgagc gtcgctccgg cacccgatct
     6781 ggaatccgat tcagagagca tatcaggcgg tggcgtcaga tgcgtcgcct tcggcttcgt
     6841 ccgcagcaga cgttgcttcc acggttgccg cactggtggt cacttcatct tcaagaccgg
     6901 taacccacac ttttaccgca gaggttatac cggggtagac tttgatctca acggagtaaa
     6961 tgcccagcgc tttgatcgga tcgtccagca gcactttttt gcggtcaacg tcgaagcccg
     7021 cttctttcag cttctcggca atcatggctg tcgtcacaga gccgaaaatc ttgtcttctt
     7081 cgccggcacg aactttgaat tcaagtgtaa gaccgcgcat tttgtccgct tcagccgtca
     7141 aaagagtgcg gcgtttttct tcacgccttt tcagaatacc cagctcatgc tcgatctgtt
     7201 ttgcggaagc ggaatctgcc ggcaccgcca gcttgcgggg aaacagataa ttccgggcgt
     7261 agccgttgcg caccgaaacc ttttctccga tctcgcccag cttttcaatg tgttcacaaa
     7321 ggataacgtt cattgccaaa ctctccctaa tttaaagttt ttcttttctt ttcatgtgcc
     7381 cgatgctgtc ttcgtgctca cagatcagga tcttttgctt tgagctgccg atgccgcatc
     7441 cgaagcaacc aggctctgaa attgatccag gtatcaaaaa aaccgataaa aggcagcaac
     7501 tgatagagat taaatagaat cagaatcaac acggcaaatg caagccacaa gctttgaaac
     7561 cggaataata ccagtgcaaa tgcggtgatg gaaattccgt tcaccagata aatgaaagcc
     7621 agcataatag cgctgttcca cacgaccagc cgcagactgt catttggata ataactgtcc
     7681 cagaaccagc ccagcgccac aacaatggca acccagacca gaggctcagg catacgcagc
     7741 cggcctgccg aatagcgcgt tcctttcagc acggcccgct tctgcaagcg cgaaaaaagc
     7801 agcagaacca gcaaaagctg gcacagtaaa atcatcccga agagcagacc gaaactcaca
     7861 tacgcccagt gcccgtccag ccatctgaac gtggctatgg cgttttccga ccgaaggtct
     7921 gatgcgttct gagacatctg ctctatgtag ttctcaaatg cctgatgcca ttccaggctg
     7981 accgccggcc acatgagtgc gtaactgccg ccgacaaaaa gaaacagcac tgcagtcaca
     8041 gccgcaaggc aacggcccgg ggtctggcgc tgcaggatca gcgtccccag caatatgccg
     8101 ggaagcagac cgaacagaag aaccagagcg agaagaaagt cgccggagac caccgcagca
     8161 cccagccaca tcagtaccgg cagaccgaca gcccggaagc gatgcccctg agaccaatac
     8221 cagcccacag gatacacgct aaataacagg acaggcagta tgccttctgt cgatctgccc
     8281 agcacaatca gtgcgccaag cagtgtgcac aaaaataaat aaactggcat agcgttgaga
     8341 agcctcgcac cggctccgaa aaaagccggt gcgaaggact ttcattatct gatcagtctg
     8401 cgagataagg caacagtgca gcctggcgcg ccagtttaat ggcacgattg agcatacgct
     8461 gatgtttcgc agtggcgccg gtaatgcgcc ggggaatgat tttgccgcgt tctgtaacat
     8521 agtgtttcag caaattgaca tctttgtagt caatatagac tacgcgatca atagtcaggc
     8581 ggcaaacctt ggcgcgcaaa gattttttct tcttacgcct gcgtgatttc ttctcacgca
     8641 ctttcatttt ggttttggct gatattctcg ccatttctgg attcctttag ttggttagaa
     8701 ggggatgtcg tcttccggaa caggctcttc gatttcgcgg ggacgctgct ccgcacggga
     8761 ctctgactga gccgactgat acgatccgcg cccgccgccc gagtcctccc agtcaagggg
     8821 agttacccga acagccgtaa ccgcaagacg tgtccgacgc tgaccgcttg cctgatcttc
     8881 ccattcactg gtatgcagcc gcccttcaac aagaaccgga cgcccttttt tcagatgttc
     8941 accgacgaat tcaccctgag ggccccaggc ggtgatatcc acaaaagtag tctcttcccg
     9001 gcgctcaccg gtcgttttgt ccttgtaata gcgcgcattt gcaacactga aacggcagta
     9061 cgccttgttg ctgttctgca gatagcgcaa ttccggatca cgggtcaaac gacccgccag
     9121 aaagacatgg tttaaatcag gtacgcgtac atcgcccatt ctgctttcct tcacagttca
     9181 caatgacagt agtatcttac agaattcggt accaggagaa aatttctcag tcgtcatcat
     9241 catcgtcccg ggagtcgcgc ccccgatgcc tgtatccgcc gtcctcgtcg tcgccattgt
     9301 catcgtcgtc atcatcgtcg ctatgactgc gacgggcagc gctttgacgc aggtcttctt
     9361 cgtattgccg ttgttgctgc tcttccaact tgagtgtctt ggcatccaga tagagtacca
     9421 tggaacggat caccgtttcc gaaagtttga aatactgctc taatttatgg acaaatgcag
     9481 gatctgcagt aaaacgcaaa acaacataaa caccctcagt gtgtttctga atggtatacg
     9541 caagtttacg tcttccccat acatcggagc gcacgataga accgccatta ttggtgatca
     9601 gctgctcgac atcagtcgtt atcgtttgga tggcactatc atccagttca ggattgataa
     9661 tgtacagcgc ttcgtatgtt ctcaaaatgt tcacctccta acttggatat tttttagttg
     9721 aaacagaaaa aaaagaaata gggagtttag tatagcaaaa aaaagccttt ctgttcaaaa
     9781 cgagggctca gccgccacca gtcccgattt tttcaaatgg tacaccggca aagctccgcc
     9841 tgttatgcct ctgtaaaact ctgtatcata atgcaagaaa aaatggcccg cccgctacgg
     9901 cgcccggttc catctttttc acgaccgtcg gagccttttc ttgtcacgtt caatgggaac
     9961 aaaagtgccg gaaacgctgt tacgtctatt gatctattaa gttttgactg taatcacagg
    10021 attattgagg agagaaaaag tgtcggatga tcaattggac ggcaccgaac cgcgcgaaga
    10081 gggagtatcg ggcagcgcgg tattcggcac agcgcgaggt ctgatacagc ggatgagaga
    10141 atgctctcag gaggcctata ttaaaagcga cgaacaagtg gtgtatctct gggcatgcgt
    10201 cacccgcacc gggttctgca accacagcaa agacaccgac catccgatcg cccctcttac
    10261 gctcgaagac tggcttaatg tggtggacga gtccgccacg ctcggtgcca actggctgat
    10321 cctcaccgta ggagacccgc tgcacctttg tcccgatgta tgggaactga gccggtgggc
    10381 gcaagaaagt tatggtatga ctgtcgggct tcatcccaaa aacgggggac tttgtgcgga
    10441 agacctggag gcgatcaagc agctggatat aaacaatacc cgcctcctgc tccgtgaaga
    10501 gtggaccgac gcacgaaaac ttgccgaaaa tgaggggatt atcgtctgga ctgccaatcc
    10561 tcagccgccc ggagaacggc cgcactgcaa gggaggtacc agaatgattt tcgtcaatga
    10621 acatggcatc ctctatacct gtggcgtcgt ggaagggaac tccaattatc atctggggca
    10681 tgttctggac aaacgcttaa aacaggttgt taccgatccg aaacggccgc gttgggtgga
    10741 aaaaagcctg catgtcgtct cggaaaactg tgacggatgt ccggcacgca tggctgtctt
    10801 tttcagctga cccgtgagtt gacaggaaaa cccttgctgc gctatactga aataacgttc
    10861 agcaacacac gaggatcttg tcatggctca ggaaatcacc cctcgccagg ccgccgttct
    10921 taattttatc gctgagtcca ttgaaaaact gggtttcccg cctactattc aggaaatcgg
    10981 ccagcgcttc ggcattgtat ccaccaatgg cgtttttgat cacctgaaaa cactcgaacg
    11041 taaaggattc atcgaacgca gctccaaggc gcgcagcatc cggctgactc ccaaggccgc
    11101 cgccggactg gttcgccgcc atgctgatgc gctgcctctc atagggcgtg ttgctgccgg
    11161 gtatccgctg cttgccatgg aaaatatcga ggaatacatt cccgtggcac cacgtctggc
    11221 acggaaaaac gctttctgcc tgcgcgttac cggggatagc atgattgatg cccatattct
    11281 ggaaggggat atcatcgttg ttgaccggga tcgtcggccc gccaccaaag acattgtagt
    11341 ggctctggtt gaagaagaag cgacagtcaa gtattattat ccgcagaacg gctttgtcga
    11401 gctgcgcccc gccaatgcgg cactgcagcc catgatcttc cctgccgaag aagtgagcgt
    11461 gcagggcgta gtaatttcac tgcagcgcga tctgggtaca gcatcagcgc cagccttctg
    11521 attcttcttt gtaaacagaa cagtcgccct taaataataa tccgtacttt atgcgcccgg
    11581 tcggtctatg tccggaacct gacaaaggaa ataatgccat gattttcgat cttgccatga
    11641 ttcagcgttt ttataaatca cttccggagc tggtagccgc tgcccgcaag gcactccgcc
    11701 ggcccatgac ctttaccgaa aaaattctct acgctcattt aagtgtgggg ctcccggaac
    11761 agccgttcat gcgcggcaaa gactacgtgg actttgatcc ggatcgggta gccatgcagg
    11821 acgccacagc gcaaatggcg ttgctccaat tcatgcaggc agggaaagaa aaaagcgccg
    11881 ttccaactac ggtgcactgc gaccacctga ttctggcgga aaacggcgca ggccccgatc
    11941 tgctccgggc actgcagatg aatgaagagg tgtttaattt tcttgcttct gtatccgccc
    12001 gctacggaat cggtttctgg aaaccgggcg ccggtatcat ccaccagatc gttctggaaa
    12061 attatgcatt tccagggggt atgatgatcg gaacagactc gcatacaccc aatgccggcg
    12121 gactcggtat ggtggcaata ggcgttggcg gtgctgatgc cgtagatgtc atggcaggca
    12181 tgccctggga actgaagttt ccgaaaatta tcggcgtacg cctcaccgga accctgaaag
    12241 gatggacttc tcccaaagac attatcctga aagtggcggg tgagctgact gtaaagggcg
    12301 gcaccggcgc gattattgaa tatttcggcg aaggcactgc ctccatttcc tgcaccggaa
    12361 aaggcacagt ctgcaatatg ggtgccgaaa tcggcgcgac cacatcagtc tttccctttg
    12421 atgagtccat ggcggcttat ctggaagcta caggacgccg ggaagtggcg gaagccgccc
    12481 gtaacgcggc atcctgcctt cggcctgatc ccgaagtgct gcaggcgcct gagtcctatt
    12541 atgaccgtat tatcgagata gacctggata cgctccatcc tcatctgaac ggtcccttca
    12601 ccccggatct tgccacgccg gtacctgaaa tgaaaaaggc ggcgcaggaa ggtggctggc
    12661 ctttgcaggt tgaggtcgga ctcatcggtt cctgtaccaa ttcctcctat gaagatatgt
    12721 cgagagctgc cagtattgcc cggcaggcaa aagaaaaagg gttaaccgcc aaagcccaat
    12781 ttactattac accgggctcg gaactcattc gcagcacact ggaaagagac ggcattctcg
    12841 atgatttcac tgctattggc ggggttatcc tggcaaacgc ctgcgggccc tgcatcggac
    12901 aatggaaacg gcatcggaac ggcgctgatg atgaacccaa caccattgtc acttccttca
    12961 acagaaactt tgcaaaacgg aacgacggca atccgaaaac ccatgccttt gttgcctctc
    13021 ccgaaatggt gacggcgctg gcgctttcag gccgcctcga ctttgacccg gcgacagaca
    13081 cgctggtcaa taatgccggt gaagcggttc gcctgactcc gccgcagggg gatgcgctgc
    13141 ctgcccgggg ctttgcagat ggagacagcg ggtttgttgc ccctccagaa gacggatccg
    13201 ccataaaagt gctggtgaaa ccggactcgg aaaggctgca tctgctggaa cctttttccc
    13261 cgaaccacgg cgacatctgc ggcatgaagc tgctgatcaa agcgaaggga aaatgcacaa
    13321 ccgatcacat cagcatggca gggccctggc tgcgtttccg cgggcatctc gacaatattt
    13381 ccaataattg cctcatcgga gcgaccaacg cttttaacga caaaaacaat accgtaaaaa
    13441 atcagctcac cggagcctat gaagaagtac ccgcaacagc acgggcatac aaagcggcgg
    13501 gtatcccgtc acttgttgtg ggcgacgaaa actacggaga gggctcctcc cgcgagcatg
    13561 ccgccatgga accgcgccat ctgggcgtgc gggcagtgct ggtcagaagc tttgcgcgca
    13621 tccacgaaac aaatctgaaa aaacagggca tgctggcgct gactttcagt gatcccgccg
    13681 actacgaaaa aatccgggaa gatgacacga tctgcatcac cggtcttgac cgctttactc
    13741 cgggcgtgcc cctcaccgtt accctgaagc atgccgacgg atcggaagag tccttcccgg
    13801 tcaatcacag ctacaatgaa acacagatcg cctggtatca ggcaggaagt gctctgaatc
    13861 ttatacggac gcagcactaa aaaccgctat ttcagcttgt tttcaaacca gaccggaccg
    13921 ccccatttcc cggtgacgat gatatccgca tcaccgtcac catcgagatc ggtcgcaatc
    13981 atattcaagc cggagccgat gccttcatcg taggaaatga catgccgctt ccaggcaggt
    14041 gcgggcgccc gttccagctc ataccaatat acgcacaggg gctcaaatgc gcccgggtct
    14101 ccgccattgt gcgccataaa gcgttttccg gcaaccagat ccagatcccc gtcgccgtcc
    14161 agatccgcca gcgccagact gtgcggctgt gaccagctgt catcaatcag atgccttttc
    14221 cacgtgattg catctccgtc acgctgctgc tcccaccaga aaataccgta atcgtgggcg
    14281 atcgcactga taatatccgc aagaccgtca ttattgacgt catagaccag aatcggcgcc
    14341 gtatgcccgg aggttccttc ttctttgccg cccagtgcca gcggatgctc gatccatgga
    14401 tcagcccgcg gattttccgg ggcctcgaac caggcggagg ggcgcagcac atccggacgc
    14461 ccgtcaccat tgatatcccc gtaccctacg ccaaattcca tttctttttc tgaaatgaca
    14521 taacggagca tctgctgttt gccgtctttc atgcccggct cataccagac cgttttcaaa
    14581 accgccggca aaacctgatt taatttgccg tcaccgtcga gatcgcataa gtcaccgcat
    14641 tcatagtttc cgttttcaac aataagatga cgttcccact gcgtatcaaa agtgcccgga
    14701 ttgcgcagcc attccgcttt catcccgaac caggacgctg tcaccacatc tgtcaatccg
    14761 tccccgtcca catctaccgg cgcattgaca aaatcccagc ggtagccttt tccttcctcg
    14821 tccacctcgc cctcgattgt gcagatctcg tatcctttaa aatcaggtgc aagatagatc
    14881 cacggcagcg caacgatatc cagcagcccg tcattattga aatcggcaac attcaatacc
    14941 tcgcccctgt agctgccgat tttatgcgca acaaaagtgg gggcggcaaa agaagtcagc
    15001 gtggcaagac agcaaacacc aaacacaaaa actgaacgca taatttttct cctgaataaa
    15061 taagcagcgt accgtacggc agataccaag aatacagaac gatggcttta tcttaagaca
    15121 ttcagctgtg cgcttcaaac ttgacccgtc ggagacctgc tgcgcacagc aaagaattag
    15181 tccagttggg acattcgggt tgaaaaaccg ctcttaatcc cgggcggtta aaatgttttc
    15241 aaccagcctg tagacagatg tactgccaaa acatgcagca ggcttccctt tagttctcgc
    15301 tcttgcccgt tctacagcct gccggacctg ttcggatgta aaatgacccg ggtcaattcc
    15361 tttgtcatat ccgggtatgt attttttcag acggcgggaa cagtcttccg catgagcaag
    15421 tttagtccct tcctcaaaat gaagcagccg ccagtattca aaattaggat tactgagggc
    15481 aatgccccgg ttctcagttg ccttttccca gacaaaaagc gtctccatct ggtgctcctc
    15541 ccactgatcc tggtcaatga caatccacgc ttcatcgccc ttctgcaaat cttcttcctg
    15601 aaggcaaagc accatacgct tcaacacata acccggtgca cttttcgcat ctccttttat
    15661 gcacttaatt ttaacaagtt tgctcgaatc cgccagcatc cgaaagtacg ccggctccgt
    15721 ttttttccct tccgttgcaa taacaaacag cttcttgtaa gaacgctgcc cgtaggtacg
    15781 cgaaaaggat cgacgaaagc gtcccatcag tcttcgctcg caccggttgt gtaaacagcg
    15841 ggatcttcca tgaccctggc aagcacaggc actcctccaa gccgcccctg gagataactc
    15901 ttcctgatat ccttgtcgta acgaacatct ttaaattcgc tgaaagaaac cagagaggac
    15961 tctccccggt cattccgttc agtcacccac atctcatcgc ggcgcagcag ccgctgatcc
    16021 atcaacccca ggtcatgggt cgtcaggata agttgcttgc gcgtcaaggc agtgcagctt
    16081 gccaaatata aatccagcag cttttgcgtc agcagactgt gaaggctgcg atccacttcg
    16141 tcgatcacaa atattttact gctcgcctca gccagcagat ccacaaaagc cggcagcagg
    16201 tctatcaggc gctgggagcc atccgattcc tgcgaaagct caaagcgtac ctcttccccg
    16261 ccagcggcag catgatagcc aaaaagtttc tccgctttaa gcgcacctgc tttgcggcta
    16321 agcagaaagc ggctgttcat cggaccgtct acacgaagtg ttttgccctc cagcagttcc
    16381 tcatgcaaca tcgcctggac aggggcaggt aagggaagat cttcaaaggc aactgcttct
    16441 gtctccagac gacgtatccc ggtatccagg tgctggagca gtctattcat ctgatcactg
    16501 aagatctttg tttgatcaaa tcgctgttca aaagcagcga accgtccgtc aggtcctatc
    16561 atttccaggt gagacttaaa ccactcgtaa actgcctgaa aatgcttcaa gttttgaaaa
    16621 acactgtttg tgaggaataa ttggttctgg cgggttccac gcgctacgaa atcaagatac
    16681 ttctgttccg gcagtgcccg gtcaaattca tagcgcccct gctccctgga aaaaagcagc
    16741 ttttccgttt tattgcctat acgaacaaga tgctcctcca gcacttccgt gcaggtcaca
    16801 gaaaatccat actcataaaa agcaccttcc gcaaacaact caacagtaaa acgggagggc
    16861 tgctggcgct tcaattgatc aagcagaaaa ggcgttaccg gtatagggct atccggccgc
    16921 ctcccctgca ccacaaattg ctgaacaaaa tgaagggcct taaaaaaatt agtcttccct
    16981 gaagcattgc ccccataaac gaccgataca ggcaggactt tcgcataggg cttcactcgg
    17041 gcaacccgtt cgctatgttg ccgttcagga gtagccaaca tagacaactc tgctgtatct
    17101 gcaaaagaaa gccaattatt tatggtaaac cttgtgagca tatgtcctcc ttacgtgact
    17161 ttatctcatc accacacaca gtttaccaca cacaacctta tttgtcaagc cctaaagaga
    17221 ttttttctca ttcatctgct ttttttatat ttttacctgc cagcgctttc tcgatttgct
    17281 ccgccagcgc ttttacttct tctctgctgc actccacaca gactttcccc ccacccgggt
    17341 gagcaaaagt caacgtaact cctttttctt cagacgctac ttgaaaacgc acgccttccg
    17401 gcattttagc gtccggcacc atccagtatt ccgcgtcaaa ttccgagatt tgcacgagca
    17461 ccccgaaggc gtccttcgga tgaacgaaga cttccttcca gcttccatcg ggataggcat
    17521 gtttgccaaa gtaaggaaca ccgtgcgctt ccagtttttt aagcattgcc tcaagattcg
    17581 gcgtctgaag cgttatatga tgaactccgc catccttttt cgccagaaat ccatcaagaa
    17641 agctgtccgg gcctgatgga gaaatcagct ccaggcggga cagatcaccc aacgccagcg
    17701 tctgccagta aaaatttctg tcggaggctt taaatttctc gccgggaacc gctcccagca
    17761 catcccgaaa aaactgcagg gcggcatcgt gatcttttac agccacagca acatgatcaa
    17821 tacgttcaat cataactcct cctttgatga agcccccgta attcatttca aatgtgtatc
    17881 aaaaaagcgg agcaacccgt agtaatccag ttcatggcca cccgggtgta catgcagaaa
    17941 gagtctgtcc gaagcatcca gacaggcata agccggttga atctgccgga aggcaaggcg
    18001 ggcaagccag ggcggaaact ggttatccgg ctctttcata ccgttttgaa actgcaggga
    18061 acggggcgct gccagcgatg ccaggcccgg atagtcgacc agcgaccgca gcccgggaaa
    18121 tttccagcac atacagtggt tctgttccat ctgatccatc aatgtcagaa agccgccgct
    18181 taccgccgca cggatacgcg tatccgtagc ggcaagccac aaggacatct cgccgcccag
    18241 agaaagtccg gcgcagccta tacgcttcgg atccaccact ttgagagaag tcaggtaatc
    18301 aacgcagcgc atcaaattcc acaagcgctc acccatgagc tcccggctca cttccagcgc
    18361 ttcatggccg cccacatcca cagccatcgt aacatagccc gcctttgcca gccgggcacc
    18421 tatccctttg tatgccgact ccggtttttc ttcaaaaaca gaatagcgcg tgtcaccgtg
    18481 cccgtgaata cagacaactg cgggaaacgg tccctccccg gcaggcagtc ccagcacggc
    18541 aggcaggcga taccccggca gtccctgaat ctccacctca caaagcacaa atccgtcctt
    18601 ttccgtacgc acctccggcg ctgcctcaag cggaagcccg gaaccctccg tcagatcggt
    18661 aatacgcagc acttcaaaaa gggctttgcg tgtttcctgc tgccattctg taaacgcctg
    18721 aacggattct gtcggtacgg gcggcgcagc aggggccgcg gcgttttccc ggagaatgat
    18781 ccggcgcggc ttgtccccgg tcagccggag cggcagagaa gacaggtctt tcccgcgcag
    18841 gtacagcacc ggattatcat ccacctgcag ggcatactgc tgatcttccc ggatcacgaa
    18901 ccattccgga aactgattga ggcggggata atcttcgggc atgtgcagat agaccgcatg
    18961 ccggggcagg tcaaagttta ctgcgccctg ccagtgccag tctgaagcaa tatgaaagca
    19021 ccacgttccc ggttccggcg acacagcacc cagacgcaga tcattgcgcc acggatgcag
    19081 ccaggcgccc tggcttttcc agaaagcata catcagtgca gtgcgtgcaa aattaccgtc
    19141 gccgtgccat ccttcaacaa tgcctgtatc ccgcggcttg tccagcagaa ttcgcgccgt
    19201 ataatccgcc cattccgccg cctcggctac aggataacga ttcagcagat tgagtccgcc
    19261 ctccaatgaa tccgcataac cgtcggcact tccgtattcc catcggtagt ctttcacggc
    19321 aggcaaattg ctcagcacat gacgcaccgc ctccgcatac cgctcttcgc catccacctc
    19381 tgccacaact gcaaaagcat tataattgta cccccagtta tccgtcagct cccggctttt
    19441 aatttcgcct gttttcggat taatcaggtc atagagcaga ccattttcat cccgggctgt
    19501 ttccagaata cggtcaagca tggcgtgcat cggcttgcgc cactgcgcat gtttttccgg
    19561 atcggtcttt gccgccacaa aatacgcctc cgacagtcca ttaatcactt cacagccgtg
    19621 atcgtccaac tggaggcgct ctgtttccgt cggaagatga tgcaggaaaa agtagtcgcc
    19681 cagctgaaac acatgcctgc ggtgaatctc ctcgcccgtc atccagtaga tacggctgca
    19741 cagctgaagc agattccccg ccacctcatg ggacgtggaa ggcagacgtc cgatttccgt
    19801 ttccaccgct ccatatgccc acaggtcttc cagcataccc aacatacgtt ccgaccacgg
    19861 gctgggaccc agcagctcag tcaccgggat caacccgtcc ttcatatatt cacttgcacc
    19921 aaaaatgagg tcgtcggacc gcacctcctg cacacgaaaa ccctgcggct caaagagcca
    19981 gtcatcggga agcctgccga ggcggttgca caggcgctgc tctgtctcaa gcattgtttt
    20041 cattctgccg tcaaaaaggg tgcggtcagt gaaataggtg gtcagcacca tgaaggggta
    20101 gttgtctgca gcggaatctt tggcattcca atacggcgag tccttgagat tcctcggaat
    20161 aagccccgtc tccgggtcgg cagtctgcag ccaggcatgc acaaaacgaa gcgactttac
    20221 cagcgcctcg cgggcaatct ctccgttttc agccgcacgc tgccacacag tgtcttccag
    20281 gctgccggat gcatgcagtg acagcagaac tgtcagcaga aaaaaccacg cccttttctg
    20341 taccagaaca ggagttctca cgaagtctct ccttttatct gcatttccgc ttagatcatc
    20401 atgcgctgca ggcggtcgca caacaaacag gagtctcttt tccgcgatgc cttatcagta
    20461 gctcacacga acccgataat tcaccgtcac ttcactgtcg gcagggatat caacggtaaa
    20521 cactgctgtc cgcgcatctt ttttcacaaa agcatgggac tgcttcagaa tctgccagtc
    20581 tccgccgggc ataggctcca caacatccag ctggatcgct gtatctttgc ggttgcgaat
    20641 ggacagctca aattcgactt cgtgtgtatt ggggcctaaa ttcttatagt ctttctgcgt
    20701 tcggtcaccc accacatcaa aagctttccc cagaatcagc ctgacctctt cattcttggg
    20761 cgtatggctg atccgatcct cgccggaaaa ctgaagcatg cccgaactgt cgggctgata
    20821 cacacgcatg attccggcag gaagcggaat gcccagcccg ttttcttctt tattttcaaa
    20881 aacaataaag gcatccgcat tctggttttt ctgtgcatcc agccgggaac tgtaaaaagc
    20941 gaggttcccg cgaaattcat attttttgct gatcttgata ccttttccgc tcaaaagact
    21001 tacctgcttc gtctgctttt ctttaatggt cgtgcggcgc tgcagcgcat acaaatgata
    21061 ttccgaaaaa gactcctcca tcataggagg cgcacccgcc atcatcatat ccatggcttt
    21121 ggcttctctg tacatcaccg gtgccggagc cgcaatgttg acatcccctg ccaccagttt
    21181 cagctgagca ttgttgtagg tagcaccgga ctggttgtcg agcgtgaccc atccttcaag
    21241 atccatggtc ttttcatctt tgcccacagt cagcacataa tctgcacgcc agctgatgcc
    21301 gccggcaaga taacttgcct ccacctcatg gtctgtagca tcattggaga ggagccacac
    21361 cagcgtgggc ttggcaataa gcgcctccgg aatttccggc agcaccacca cgcccggatg
    21421 acccagataa atcttgccgt ccacctcata cacgggtccg ttatttacgc tcagcagttt
    21481 cgcgggctgc tcataaaaac tgtaatccgt gttcttgttg atcagggtaa cattgcgtcc
    21541 cacatatttt tccatcagct tgtcagggct catcaggtca tattcataat tctgttcaag
    21601 tattttcaaa gcgcccggat cgttcaacga acgaagactc actgtttccg gaaggatcgt
    21661 tgaagcaaca tccatgaaac gaagcgctgc ttcccccgga agaagacgaa ttttccggcg
    21721 gtcacggata agcgcccgat cgttattgta gatcgtgatg gcaacatcgg tctgatcgga
    21781 cagcgtggtc tgcccgcttg cgaccttctc cgccacagga tccgacagtt tcagcgaatc
    21841 tgaagcctgt gcctccgctg atacgccgga cagaatcaat gcagtgaaaa ggaagagaat
    21901 aatgtggggc gctttcatgc tcgtctcctt gatttgttag ggctgattcg tcaccgcttt
    21961 cattgtagca caatggaaac accgttgcag ccgataaaat gccgtccggc tctttttgtt
    22021 ttttcacgcc aatcatgata tactgaacca ctgcctattc tcacttttcc cttatctttt
    22081 cacaggggca ggactatatc cattcccaaa aacaggaggt tgttgccatg aaattaacag
    22141 gtaaagtttt agttgcgcag ggtggcggac cgaccgcggt catcaaccag agcgtcgtcg
    22201 gcgtcgtcct ggaagcacgt aaatatccgg aagtgacccg catctacggt gcccgtcacg
    22261 gcgttaacgg cattatcaat gaaaactttc tcgatctgac cgatgtgcct accgccaatc
    22321 tggaagcctt cgctgcatct ccctcatccg gcctctattc cacccgtgac aaacccgacg
    22381 ccgcctactg caaaaaaatc ttcgaaatct gcaaagccca cgacatccgc tatttcttct
    22441 attgcggcgg aaatgacagt gccgacagct gtaatactgt cagcaaagag gcaagcaatg
    22501 ccggttatga gatgcacgtc atccatgtgc ccaaaactat cgacaacgac ctgaaagtaa
    22561 cggatcattg cccgggttac ggctcagccg ccaaatttgt tgcccaggct tttgccggtg
    22621 ccgatctgga caaccgcgcc cttcccggcg tatatatcgg tgtggtcatg ggccgtcatg
    22681 ccgggttcct gactgctgca gcagcactcg gacgcaaata tgaggacgac ggcccccatc
    22741 ttatttatct gcccgagcgc actttcaatc ttgaacgctt taaaaatgac gtcgagaacg
    22801 tgtacgcaaa atatgggcgc tgtgttatcg ccgtttctga aggtattgtc gatgaaacag
    22861 gcaaacccat tgctgccaaa tttaccggcg gagaaaagga tgcccacgga aatgtccagc
    22921 tttccggcag cggcgcactg ggcgatcatc tcgcaaaata tatcaaggac gaagcaaaga
    22981 tttcccgcgt ccgggcggat acactcggct atctgcagcg cagcttcctg ggctgtgtga
    23041 gcgaagtcga tcaggtcgaa gcccgtaccg tcggcgaaat gggcgtacgt tatgcgatcg
    23101 gacagcagaa gagcggatct gtcgccatga aacgcgcagg caactacagc atttcctatt
    23161 tcctgacagc ccttgacacc gttgcccggg actccattga aatgcctgac gaatatatca
    23221 acgctgccgg caacgatgtc accgaagcgt tcatcaagta tgcgcgccct ctcgtcggga
    23281 cgttgactga agcaaccctc attgacgcac cggctgtgca aaaaattctg aaaaaataac
    23341 agcctttact gccttttttt tctgccgttc cggattattt ttcaagacag tccggaacgg
    23401 cttttttgtt cttttaacgg gaaagcatat tgcgctgaac cggtgctaat tgctatgcta
    23461 gaagaaagga ggttttatga aaaaaacgct gcttctttct ctggttatgg tcgctggcat
    23521 catcgtgaca ggatgcggta aacaagccgc cgcccccggc gaaaccgctg acaaggcgcc
    23581 ggataaactg cgcattgcgc tggtcatgaa gtccctcgcc aatgagttct ttctgaccat
    23641 ggagcaaggc gcacgggcac atcaggcagc ccatgccgat caatatgtgc ttctcgcaga
    23701 cggcattaag gatgaactgg acgtcaacag gcagattcag ctggtcgagc agatgatggc
    23761 gcgtcacgtg gatgccattg tcattgcacc tgccgattcc aaagcactgg ttcaggtatg
    23821 ccagcgtgcg caggaagcag gcatcattgt catcaacatc gacaacaaat ttgatgacga
    23881 aatcatgaaa cagcgcggcg ttcatattcc ctttgtcgga ccggacaacc gcaaaggtgc
    23941 acgccttgca ggcgaatatc ttgctgcacg cctgcagccc ggcgatcctg tcgccattat
    24001 cgaaggagtg ccttccgcct tcaacggaat tgagcggcga ctgggcttcg aggctgctat
    24061 gaaagaggca ggaatgaaca tcgtcgcctc tcaggtcggc gactgggaaa tggataaggc
    24121 gcatcgggtc gtctccgccc tgattaccga gcatccggat attaaagcct ttctctgtgc
    24181 caacgacagc atggcactgg gcgctgttgc ggccttgcgt tccgccggaa aactgcaggg
    24241 aattcctgtt atcggatttg acgccatctc cgccgccgtt gacctggtta aaaaaggcga
    24301 acttcttgct accgttacgc agcatgcaga ccggcttgct gttttcggta ttgaagccgc
    24361 actggaagtg cttaaaacaa aagcaacacc ggaagatatg gaaaccccgg tggagcttgt
    24421 gacagcggaa gcactcgcat catgatacag ggacgacagc tgctgagagc gctggtaaat
    24481 tacgcaggtc tggcattggt actgggtctg ctcgttacac tctttgcgct ctcaaccgat
    24541 tatttcttta cctggaccac cttccgcacc atagccaacc agatccccga tgccgtcatc
    24601 ctcgcaacag gtatgacatt tgtcctcatc attggcggca tagatctgtc cgtcggttcc
    24661 gtgctggcgc tgagcggtgc cgtactgggg cttgccatgg ttcgctggca gctgccgctt
    24721 cccgtcgccg tgctcctctg tctgggtacc ggagccctct gtggcgtgct taacggtctt
    24781 tttgtaatcc gctggcagct gcccgctttc atcgttactc tgggcatgct ggaggcggca
    24841 cggggcggcg cttatctggt gaccggctca aaaacgcagt atatcggagc aggcatggac
    24901 tggatcagcc ggtggagcct tctcggtctc tccctgccct ttctcgtagc catgatcacc
    24961 gtaattacag gtcaactggt gttgacctac acgcctttcg gtcgaagtct gtacgccatt
    25021 ggcggcaatg aagaagcggc gcggctctcc ggcatcccca ttggcagagc aaaagtcgcc
    25081 gtctacgcct tgtgcagcac tttatccgct ctggcagctg tcatttactg ctcccgtctc
    25141 accgccgctg accccaatgc aggcagcgga tacgagctgc aggccattgc tgcagtcgta
    25201 atcggcggca ccagcctcat gggcggtcgc ggctccgtca tcaataccct tttcggcgta
    25261 atcattatcg ctgtgctgga aagcggcctc atccagatgg gcgcacagga accggtcaaa
    25321 cggctcgtaa caggcggcat cattgtcgcc gctgtcgtca ttgattacta ccgcggaaaa
    25381 atctgagcac acgtaaaata ctttccgctc cccacttgct tacgctacta gaatatcctc
    25441 cggcgggcag agtatgatag tagtgttttc atggagaatt ctgatgcgct actttcttcc
    25501 ccttactctc ctcattcttt gcggctctgt gtctgccgat gatgcaggat cctttgtctt
    25561 ccttgatgac ggcgacaata ccggtgttct gtatacctat gcatcgacgg aaattcagga
    25621 ctattttgca aaaatgttca ccacaaaact ggtgacagaa gttcccgaag gaacggcacc
    25681 ggacgttgtt atcagactgg gcacgccgga cagcaataca gccattgccg atgccgttgc
    25741 tgccggacag ctcgtccttc cggaaggcgc actctccgac caggggtata ccctgaaaaa
    25801 tctggaaaac cagctcttta ttgccgcccc ggcacctcag ggcgtgcttt atggcaccta
    25861 tgcttttctt gaagcttacg gcgcctattt tcagatcagc ggagaacggc ttccggctcc
    25921 ccttcctttt acgctcaaga cactggatct gacagaaaaa cctgccgtca aattccgcgg
    25981 tctcctcccc tgggatgcct atatctgcgg cattgccgga tacaacccgg aagattacag
    26041 acagcttatc gaccgggcag ccaggcttaa actgaatttt cttcagtttc atttctaccc
    26101 cggcagtgct tttttcacgg aaaccattga cggcacaaca gccgagcccc gttatctcgg
    26161 tatgcctgtt gacatcttcc ccaccgcaaa cgcagtcgga aaagccgctt tcggcgatgt
    26221 aatcgccttc ggtgcacagt gttatatcga tcatgcagga agccccgctg cacaggcgca
    26281 cgccgtgcag gatatgatgc gcgacgctct tgcccatgca aaaaacaggg gtatgcacac
    26341 cgctgtcggt atggagctta tgcagccttc cgtgggcagt cctcttttta cggcgaagcc
    26401 ggagggttcc gtaaattgcc tggacccgct caggccggag aatctggcac tgtcccgcca
    26461 gcgctatctt tccctcaaag agacctatcc ggatgctgat ttctactgtt tctggcagag
    26521 cgaaggacag ggtgtacgcg gggcagccgt cggccaggaa gaaggtgccg ctgccatgcg
    26581 tgaagcgcag gcgcactgga cctctcagga caactatcgg ggtgacatcg attatgcctg
    26641 gctgctcaag cagctctgcg acagcatgac ccccgaagaa caggcgacat ccgttgcggg
    26701 cggctggttt ctgcaacatc tctttcccgg cattcatccc gactttccgg caggcatccg
    26761 cttctcctcc atcaacaatt attctatgga gcaaagtctg gacggcgggc tggattatta
    26821 tgctgttgca gcagaaaata gggcggtatg ggtcactgac tggtgggaat acagcggtga
    26881 gcagtggttt ccccagtggc gtgcaggagg gcaggagcgt atttaccggc aatgcctgga
    26941 ttacggcata gaagcccttt cgctcgtggg ctggaaattg tccggcatcg agcacaatat
    27001 ccggtttatg gcggactttg cctggcatcc ggggcttacc gccgagcaat tttatcgtga
    27061 ttatgtgagc aggctgtacg ggcaggactc cgcaccgctc gctgatctgt acatgacagc
    27121 cgatgccaaa tcagagctga caccacctgc gacttcttct gatgaccggc ccatgtctct
    27181 gggcgaaggc tggattgtgc tgggcatccc gccctttccg gataccgcgg aagggctcag
    27241 cacggaggaa tggcgccata cagcggaacg ggcggcagcc attgcagcgg aacagcagac
    27301 atttctgcag agcgaccgtg acgctgtcgc gcttatcggc cagattattc cggggctgga
    27361 cagtcagggt cagtactggg cggcgctgct cctcaatcgc ctgcgcatgc gtctccatta
    27421 tctggaatcc atgatcgcac tgaatgaatc ctacggtatt tatcaggcag ccgcgccgca
    27481 gggcataccg gctgcgcggg cagccgccgc tcttcgcctg aatgacgctg ttgctctggc
    27541 tggcgaggca atagaaatat acagcaagga aattcgaaac cggaatgacc tgggcgtcgt
    27601 tgctcaaatg aatgtgcagt atctgcagcc tctggttcag ctgcatgcgt ccctttccga
    27661 ctaagttttc catacagcaa ctacaaggag tgtttttatg catcgttttg tgatcctggc
    27721 tctgaccttc ctgttaacag cctctttttc ttatgccgac gatgatgacg gcatcatggg
    27781 caactatcag ggcgccttca ccagcccggg tgccgccggc aaaaccgtac gcgcacagat
    27841 tgtcgcttac ggaaaaggca actacacgct cgttcttttt ctcggcgatg aaaaaggcga
    27901 gttctcacgg gatgagatca ggagccacgt taaaaaaggg cagctttctt tcagtaagtc
    27961 gatccgtgtc gctccctggg gaggaaattt tacactgacg gctcaggcgg cagaagaaac
    28021 cctggccgga acactgtcac cgggaaaaaa gacttccgcc gagtcgctga gctttgaatt
    28081 gaaacgtgtt tttctggaac cgcctacacg gggcatggcg ccgcccgaag gtgcacgcgt
    28141 cctttttgac ggaaacaacc tcgatcagtg ggaacggcat ccccgcgtct ggaacattca
    28201 ggacgacggc gggatgcagg tttgtggaag cagtctgaaa acactggaaa acttcggaag
    28261 cgggctctac catgtcgaat tcatgacccc cttcacgcct gaaaaacgag gacaggcaag
    28321 aggcaacagc ggcgtttatc tgctgggcat ctacgaaatt caggtgctgg acaccttcgg
    28381 gctcccgccg gcagacaacc actgcggcgg tctctactct ctggcaaccc ccggaaccga
    28441 tgccctgctc ccgccgctgc agtggcagac ctacgacata acctttgaag ccgccgagtt
    28501 cgatgatgcc ggaaacaaaa caaaaaatgc acgcctccat gtcctgcata acggcatcgt
    28561 cattcatgac aatatcgaat tgcccggacc gacacccggc ggaatcagcg atcaggaatc
    28621 tgcctcaggc ccgctgctgc tgcaggatca ccgtgatcct gtgcgcttcc gcaacatctg
    28681 gtttaaaccg gcatcctgac ggctcttgga cgtgtcaccc gcttacggat aagcacatgc
    28741 acatttcact cgttatccct gcattcaacg aagagagtcg catcaccggg acgctggaaa
    28801 aagcagatgc ctttttcagc cgtcagccct acgatgcgga aat
//
LOCUS       DAOPWB010000020        28794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1585_length_28794_cov_5.832806, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000020 DAOPWB010000000
VERSION     DAOPWB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 28794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28794
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1585_length_28794_cov_5.832806"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            419..3649
                     /locus_tag="PKN92_03380"
     CDS             419..3649
                     /locus_tag="PKN92_03380"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HNZ47867.1"
                     /translation="MFRSDWMTLNGVWEFAETDGNETYLDSRLYPDQITVPFCRESAL
                     SGLARTQFVKNVWYRKMVSLPPSWQGKQIFLHIGACDFRTTLYVNNKEAYVHQGGSSP
                     IRADITAFLQSGENTLIVHAFDDVRSGLQAGGKQSPQPESYGCMYTRTTGIWQSVWLE
                     AVDPLHIVSYRVAADRGRGGALLDVNLSGVAEGLKLTVRAFSDEVPISEATVRCAGSS
                     TSLFLSIPAPRWWMPEDPFLYDLELRLEEGDRVCDIVSGYFGMRTVELKGRSFLLNGE
                     AIFQRLVLDQGFYPDGIWTAPSDEALKKDIELSMAAGFNGARLHQKVFEPRFLYWADR
                     LGYMVWGEYPNWGLEPGNPASFDTVLAEWAAIIARDFNHPSIIGWCPFNETTAKDIPL
                     QAMVVDLTKRLDPFRPVLETSGYTHGVADPQLLDAHDYDQNPETFRKRWTAALCSQEL
                     PRRYGGLSVEVPFFVSEFGGIGWNVNKGAWGYGNTPQSLEEFYERYNGLTAALLDNRY
                     CFAFCYTQLTDVEQEQNGIYDYHRNPKFDLAPVIKANTRKAAYECDPPLGLPSDSDAM
                     QVLVGAAPDGDCASAWKYTMEKPAKDWMTVSFNDHAWKTGAGAFGKKEGSEHFIRTLW
                     DTEQIWLRKSFSCTHDELDKAFLVMHHDDDVEVYLNGKPLISLDRWNDRYEAFDVTEA
                     VCSAMKKGANMLAIHGRQHHGGGQFIDAALLVSPKMKKADEMNIEKRTFGTTASGESV
                     DIYTLTAAEDFKVEITNFGGIIVSIQTPDRNGSLADIVLGFDSLDKYEGKHPYFGAIV
                     GRYGNRIANGRFDLDGRTYTLAQNDGENHLHGGIIGFDKAVWKAETLRREDAVGLVLT
                     HRSPDGDEGYPGNLDCTVTYWVTRDKTLEIEYEAVTDKATPVNLTNHSYFNLDGHDSG
                     AILNQTLAIYAEYMTPVDSTLIPTGELRPVAGTPLDFRTPVAIGAHIDTADEQLRFGK
                     GYDHNFVIEKDPQGRMKRAARAHSARSGRVLECWTTEPGVQFYCGNFLDGSDVGKGGV
                     VYHHRNAFCLETQHFPNSPNQPSFPTTILRPGEKYDSKTVYRFSAE"
     gene            3717..5522
                     /gene="pepF"
                     /locus_tag="PKN92_03385"
     CDS             3717..5522
                     /gene="pepF"
                     /locus_tag="PKN92_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNZ47868.1"
                     /translation="MSDCKSPLPRQKVLEEHTWNLKPLFKSDAAWEKAFKKAESELPA
                     IGVFKGKLSKSAAKLLACCRLSTSIHLNLEKIGAYAQLKYAEDITNPLYQGMVARFSR
                     LAMLASEAESYINPEIQAIPRKIMDGYLKDPILAEYKALLKDLLRYRPHVLTPAEERL
                     LAMQGEVSEAPSKIFDQLTDGDLVFGTVTDENGKESALTQSSFVTFLESPKRSVRKEA
                     FEKYYQVFESHKYSLASILNASLLQDIYYARARNFPSARESALFADRLPVSVYDSLIT
                     AVRDGLPSVHRYYALRKRVLRLKDIHFYDTYQPLVKGMSVSIPYEEAVETICDAMGVL
                     GNDYVGALRKGLTADRWVDRYENAGKMSGAFSYGVYGCPPYILMNYEDKVLNSMFTLA
                     HEAGHSMHSYYSMRRQPPQYASYPIFLAEIASTFNEQVLGHRLLEKAKHKKERTFLIN
                     KQIDEIRGTIIRQTMFAEFEKRIHEQAEAGIPMTEESFRQIYRDLLNDYFGTGFAIDD
                     SLELECFRIPHFYRAFYVFKYATGLSAAIALAQQVLGGGSREREQYLQFLSAGGSQPP
                     LVTLKKAGVDLTGPDAVCDAMTKFSNLVDELESLL"
     gene            5561..7162
                     /locus_tag="PKN92_03390"
     CDS             5561..7162
                     /locus_tag="PKN92_03390"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNZ47869.1"
                     /translation="MKRTISVQSGVYRCALLLSTLLLPLAGMALEPEQRQAAQSQLSL
                     LTRALDFEAQRASSSNKDLGRNGDAHSILPGESLTLLDAAGPGIVNHFWNTVGTADPF
                     YARSLVLRIYYDGQESPSVEAPLGDFFGVGHGAAKELDSIPVSVSSLGRSRVCYWAMP
                     FRKHIRVVVANENPAVKVDSFYYYLDWQKVDQLPEDTLYFHARYRQEAPASEGNYTIL
                     QTEGRGHYVGTVLSVHQMETGWFGEGDDFFYIDHAETPQLKGTGTEDYFNDAWGFREF
                     CRPYHGVSLYEGVVVGDRVTAYRWHILDPVPFKESLRLEIEHKGSIFDEEAPIAKFEI
                     GGFIERSDWVSSVAFWYQYPPVNSDEPFPAWADRMPPYKIISASDLSYKADPPLLVLP
                     LSGAILYAPMSGTGEITYTFAIEEKGTYRLDGLFTHSLMCGVYQPYLDDAPIGAPVDF
                     SAPGFDPLWMHLDTLILDAGEHHLKFVGTGATPRFYRSMAPLHNGIGILSLSVLHLEE
                     MAGFKVALKKEQDKKELPHDQNTGR"
     gene            7140..8279
                     /gene="pdxB"
                     /locus_tag="PKN92_03395"
     CDS             7140..8279
                     /gene="pdxB"
                     /locus_tag="PKN92_03395"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF001309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HNZ47870.1"
                     /translation="MIKILADENIPYGIDAFSTLGTVQLVAGRAISQEMLKDKDALIV
                     RSITKVNKELLSGTAVRFVGTCTIGEDHVDKQYLEEQGIAFSSAPGCNADSVADYITG
                     ALLLLENHFSLALSQMTLGVVGVGNVGSRVVKRAQALGMTCILNDPPLAEKNGNSGYA
                     SLEEIVKCDIITFHTPLIKEGKWPTRHLVNEAFIASLKPETIIINTARGAVADNAALK
                     SALRSNRIRAAVLDVWENEPSIDPELLALTFLGTPHIAGYSFDGKVNGTTQIYASLCS
                     FLGVDAQWKADAVMPPPLCPEVTVDPDDPNCLLHAVSKVYDFKEDDRRMRELCSLPPD
                     KIGVHFDLLRKQYPVRREFHNTSALLTRQDYALTSSLSGIGFTVT"
     gene            8373..9629
                     /locus_tag="PKN92_03400"
     CDS             8373..9629
                     /locus_tag="PKN92_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNZ47871.1"
                     /translation="MVKNIIKQDGYLTNEEIASFVQETLSDFPLTGKRVLFIIPDQTR
                     SMPMPVMFRALFEAVNGRAAKMDFLIALGTHPPMSNTMIEKLLGISDEERKGIYKDIG
                     IFNHEWQNPDALTCLGEISAEEIGTLSGGLLFQSAKVTINKRILDYDHLIVVGPVFPH
                     EVVGFSGGNKYFYPGISGAEILNLFHWLGALLTNYAVNGTKHTAVRAVVDRAASMIPV
                     EKHCFCLNVSGTATKAIYFGSPEEAWSEAADLSAQTHVIYKDKPFQSILAMAPPMYDE
                     LWVAGKCMYKLEPVVADGGELIIFGKHIREVSVTHGELIARIGYHVRDYFVKQPDKFR
                     DIPGGILAHSTHVRGMGSFENGIEKPRVQVTLATSIPEAECLAINLGYRDPDSITPED
                     WRDQEDQGKLLVPNAGEVLYRLTSYV"
     gene            9667..10950
                     /locus_tag="PKN92_03405"
     CDS             9667..10950
                     /locus_tag="PKN92_03405"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNZ47872.1"
                     /translation="MKKNVLVAQSGGPSPVINSSLRGVIDTCKMFPDIFGTVYAGWHG
                     IEGVLKEELLNLSAQTDEEVALLRYTPAAGAIGTCRYKLKSSQKEDFQRVLDVLKAHN
                     IGYFFYNGGNDSMDTANKVAKLAIEQGLDLVAVGVPKTIDNDVGDSEFKLIDHTPGYG
                     SVARYWMSMVQNANEENAGSSPADPVLVLQAMGRRIGYIPAAARLADPKREMPLQIYM
                     AESKISAQELCDRVNDQIRKDGRCLVVVSEGFDVGELGEVKDSFGHTSFGSSQMTVAQ
                     KVVNLLNKHGLPAAGSARGQVPGTDQRATTAYASIVDLDEAYKVGQQAVLIAVSGENG
                     WMSTILREPGPIYNVRYDKVPLESVANSERSFPDAWISESRTDVTDAFVAYAKPLIGE
                     DWPSVPVINGRQRLCRFEPIFADKKLPPYTLEALR"
     gene            complement(10961..11803)
                     /locus_tag="PKN92_03410"
     CDS             complement(10961..11803)
                     /locus_tag="PKN92_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ47873.1"
                     /translation="MRSSVDITVLCNNDSIGGHIRAEHGLSLHVSTPSGNLLFDTGQS
                     DVFLDNAKTLQISLDKLSALVLSHGHYDHTGGLAYGAALFRNLPIYFHPEALQDRYRR
                     MDDGTAKSIGIPCSSFNLLQHPDFRAVHTSQPVEVLKNIRVTGAIPRTASFEDTGGPF
                     YLDRECTLEDSIADDQALWINASCGVIVLLGCAHAGVINTLRYIQTLILPRKICAVIG
                     GFHLKNASEHRLLTTIDALKRFDLAMLAPCHCTGEKAIEALAAAFPNIFVQCNGGSRF
                     TFPL"
     gene            complement(11849..12736)
                     /locus_tag="PKN92_03415"
     CDS             complement(11849..12736)
                     /locus_tag="PKN92_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNZ47874.1"
                     /translation="MNQKTSHTASDCNSCSKQDCAAKSRLSHESEQEFTDRQLLMERM
                     CQIKHKILVLSGKGGVGKSTVAVNLCASLSLAGKRVGLLDVDIHGPSVPKMLGLEGMT
                     VQNNEGAIVPVEVGDMKVVSIGFFLRNADDAVIWRGPMKIGVIKQFLKDVEWGELDYL
                     IVDSPPGTGDEPLSVCQYLPDADGAVIVTTPQDVSVADVRKSINFCKQMKMPVLGVVE
                     NMSGFICPGCGERHDIFKSGGGERMAKDMGVPFLGSIPIDPDMGRAGDDGTPYTQAFS
                     ASAGAKAFEKIVQALTEES"
     gene            12979..14430
                     /gene="gnd"
                     /locus_tag="PKN92_03420"
                     /pseudo
     CDS             12979..14430
                     /gene="gnd"
                     /locus_tag="PKN92_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
     gene            complement(14533..14670)
                     /locus_tag="PKN92_03425"
     CDS             complement(14533..14670)
                     /locus_tag="PKN92_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47875.1"
                     /translation="MENKGLFFALQTTILYPFAYVIGVIAGLLWYPISIFEALFDPNT
                     P"
     gene            14979..16409
                     /gene="pyk"
                     /locus_tag="PKN92_03430"
     CDS             14979..16409
                     /gene="pyk"
                     /locus_tag="PKN92_03430"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNZ47876.1"
                     /translation="MRTNCFTKLVCTIGPASESPEVITEMLLAGMSVARLNFSHGEFD
                     WHGTMIRRLRECAAKVGRSLAILADLPGVKIRVGCMKEEGVQIVEGSTLILTTEEVEG
                     DTKRIQISLKKLPSTLKPGDQVFINDGIIHLEIMEIEGNEARCRVLVGGELRSRKGIN
                     LPGIDLDIDAITEQDLKCLKFALNQGVDAIGQSFVTSAKDVECLRNAAAAMGFSPFII
                     AKIERSQALEAMEAIMYAADGIMIGRGDLGVEIPLETIALAQKELIYRANLLGKPIIT
                     ATHMLDSMTCRLRPTRAEVSDVANAILDGTDAVMLSEESATGSYPVEAVTMLSKIAVA
                     TEASRPKECPWEHLIDRGVRKDTAFVDYISRNVNHAVDHLQPDAVVVPTSSGYTARMV
                     SRFKLPLWIAAVTPSPRVSQGLIFSYGVEPVLTQESITDWTAFSKEWIKSLNAQKNGL
                     GILLQGPSPSHPETNPSMEIILPDKG"
     gene            16759..17766
                     /locus_tag="PKN92_03435"
     CDS             16759..17766
                     /locus_tag="PKN92_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ47877.1"
                     /translation="MFKKHKGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQ
                     NNLKQWGIVFKMYSGESPGEKYPPITAHYMPLGLVDCNLEGGTQRNFVIAAGVYIGAV
                     YPEYLTDPGINFCPSDPNNKPTEAYATVDFPNPLTGQTVRKGDPIFGIPCMQMTKGMQ
                     NSDESYWYTGFVFDMLEDTFNTGIQAFAGPRQMLEAAIQLDLLGLITGSSSPQTVESR
                     VDKDVKVTPGFGNGGSYNDPLNGRDTVYRFREGIERFMITDINNPAASAMAQSTLFIM
                     ADGVSTNLSEFNHVPGGSNVLYMDGHVEFRRYEEKGQGPVNGPVARFVGYLMSGANLS
                     I"
     gene            17912..18295
                     /locus_tag="PKN92_03440"
     CDS             17912..18295
                     /locus_tag="PKN92_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47878.1"
                     /translation="MPTSTFACMINCMDGRVQEAVIRHLKARLGVDFVDAVTEPGPIA
                     HLSKGASVWVDNIKACTDVSVEKHNAKALAIVGHFDCAGNPVSKEEQVKQLHQSVQVL
                     KEWYPHLPIYALWVNEQLEVEEIVS"
     gene            18421..20634
                     /locus_tag="PKN92_03445"
     CDS             18421..20634
                     /locus_tag="PKN92_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ47879.1"
                     /translation="MLSNPKETHPIFAHKHYILLACILLAGLAIRIAYHSEHVHAPDY
                     ASPVLDPQLNDYWARALVSGDWTPPPHADDPKIRTTPYGRPPGYPWILAGIYTLFQES
                     YHGPRIVQTVAGLCVILLLCATAHSLFNAEVALLSAALFAFYWGSPYFEGELNSPIWE
                     LLFLLAVFFLFLKYCKTSKKSYMFLSGLLGGISLLIRPNLLVVLFFMIGYLLVTAGVA
                     RQKLQTLLLNAGLFVFAVAIPISPVLVRNWQVSGDFVFISYYGGVNAYIGNNPKADCV
                     SPTIPDLYETAGVDAWNCFNYPQLVEGLGRRMNKSSLSFSQASTYFYKKALDFWRQEP
                     QQALWLTLKKAWYFWSPHIISDSKVVYYERKSSPILACLPGFTLISALAFTGMMFFPL
                     AEWRKKRSLHLLLLFGLSYFLSILPFFMSERYRFPIIVFLIPFSAYAVAIIKSALLGK
                     EWKRCFHVLSVFCLILAAMSLHLFPYKPDESVWYLHKAFAYAGNKKTNEALSCLEKSL
                     ALNPASGEAMLQAGYIYASKGEHERAVALYLKALEIKPHSRYLLNNLGYEHYLSKEYD
                     RACDYYRRALEVSPRYTLAYNNLGNALLKLGNYEEALVNYDRALAIDPKDPFAECNRG
                     NLFLETERYQEAAAAYRMALAADSGNADAANNLGLALLRDGQAEDSLEWFEMAWRLRP
                     PYPLAHLNAAKAYRQLGLTEEARRHYKLCLEYWPENEEVREELTLLQGQENRQME"
     gene            complement(20594..21703)
                     /locus_tag="PKN92_03450"
     CDS             complement(20594..21703)
                     /locus_tag="PKN92_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ47880.1"
                     /translation="MKKYLQITAGLAIGAFLLWFLFKDTDWVKVWRAFQEARTGWLFV
                     GLVAILISFVIRIWRWSYIVNPVIHVPFWQLFSATQIGFLGNFVLPARIGEVIRALVL
                     SKSRDIPFPKTMAFVAVDRLTDLFGLVYVFLVTLLVFRPNSDVYLPEDMKALYSGAIS
                     KEMVQGSLFVATAALIAGVAFLMLLIARKKFFLALSDSFFALFSKRLGHFVRKLFLNF
                     AEGMQVLLSFKDLLKASLVSLLLWTFFAISQYAVFKAFDLSVPWFAPFVVLSLLSVFI
                     SIPGPPGFIGPFHVGVVGGLILVNNALDLDSLRAVAIVAHLFNLVPIVLIGIVCLSFE
                     KIGLRQLAAEGDTLTVHENAAASTPSADFPDPAAK"
     gene            complement(21737..23272)
                     /locus_tag="PKN92_03455"
     CDS             complement(21737..23272)
                     /locus_tag="PKN92_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNZ47881.1"
                     /translation="MLAIIQSSAVVGIDAYPVAVEVHTQKGLFRYKVVGLPDTAVKES
                     EDRVQSAVKNSGFKVPEGKTVINLAPADMRKQGSALDLPIALGLLAATGQLTTDKISD
                     YLIAGEMALDGSVRPITGALAMAIAARNTGLKGLLIPVENAEEAGVVDNIDIIPVDTL
                     HNAHAFLKGELDIKPFVTDLENVYRDSARRIMDLADVKGQAHVKRALTVAAAGAHNLL
                     MVGPPGTGKTMLASRLPGILPELTFEEALETTRIYSVAPGIHMRNKLVLNRPFRSPHH
                     TATTVSITGGGANQPIPGEVSLAHNGVLFLDELPEFNRSALEVLRQPLEEGEVHLRRA
                     SYSITLPSRFMLIVAMNPCPCGCRTHPQKRCKCTVGEIQRYMSRISGPLLDRIDIHID
                     VPPLRFEELHTTAKEGPNSAEVRASVKAAREIQRSRNGSKNSWNAHMDTSQLRKHCRL
                     SESAMSFLEAAVLRMGLSARAHDKVLRVARTIADLDGDEAIAEKHLAEAVHYRSLDEE
                     VFF"
     gene            complement(23414..25957)
                     /locus_tag="PKN92_03460"
     CDS             complement(23414..25957)
                     /locus_tag="PKN92_03460"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNZ47882.1"
                     /translation="METIKSVLVACSIIVFCVVSLFPLSLSAEALSLIHDGQSDYSIA
                     VSSEASPSEKFAARELQRCIRASAGVLLPLIQSDLAKENMPLLLVGFSETATRLGISE
                     SLEDVGDQGFLFRSVPPHFFIGGSREAGTLYGVNAFLEEFLSVRWYAPGEETIPEIST
                     LSLPEMDKIQKPAFKWRHTSYAWPGKDDLFLARLCDNDGMGTGEREQGIQYTQDGRCH
                     TYFRFISPSEYFDSHPEYFSEIGGIRRKHETQLCLSNPEVLEIVTEKMLERMRAQPEI
                     TQHNFSQMDYYNYCECPQCTAINERYGTSGGTQYWFLNQLAERTSVFFPEKTIGTLAY
                     MYTEEPPEGLALHPNIAIWLCHMFPSCDSHSIATCPHNRQYRERAKRWAELTKHLYIW
                     HYVVNFVHYYSPFPNFGALAEDMRFYKNIGVEGIYLQGMGHSGGGGEFSLLRPWYGMK
                     LLWNPDYDAQELLQDFLQGYYGKAWNPVYRYINMLQDEVDKNNIHMHLYTNPAQGYLP
                     DEIIDAGNRLFDEAESLAMSDDNLLEKIRVARMPLHYACFFPRNGYRIEEEQLIFNEP
                     LADFDTISSFVSRLEKHGFKTIREVQGDPQQLFLLGLAFSSPLHAPKISSKYLEADFA
                     PFLGGRALRVTDKNTGLCVTGYNITRNLFFPFCGGEELRLGGEFDPAGMFYQFEIVSR
                     KDNALTLQAEADGWLITREFTLHPDNPVLTIKATVKNLLASPREAMMRSHTNFHLGAL
                     QQFNVHFTDLENNPHTHQADEVIPGLREGIKFRKKEIPLNEWCLRGDIPLEVIQRFDS
                     EQLDHAWLYAYPEYINDLECELWAKAVLLQPGESHSFTVDWEIRPVSLP"
     gene            complement(25985..26869)
                     /locus_tag="PKN92_03465"
     CDS             complement(25985..26869)
                     /locus_tag="PKN92_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNZ47883.1"
                     /translation="MKRMHWKISLHGGHSSEYCDHAQDPLPALVAAAVDAGISIYGIT
                     EHAPRFKTEHMYSEELALGWTPEILLNKFRRYAHELDSLSRIWDSKITLLKGFEAEVI
                     PCDSYADKMLSLKRELGFDYIVGSVHYVNGISIDYTRALFGEAVQRCGSLENLCIAYF
                     QTVRNMAEALRPEVLGHFDLIRKMAGNTSELQSAPVLRAAENALHSIREIGCIIDINT
                     SPLRKGFACPYPAPHFLKMIREKGIPVCFGDDSHSAAEVGAGFETARQYLIDHGIREL
                     TALNKKPGKMEKETVLLQ"
     gene            27154..28194
                     /gene="ilvC"
                     /locus_tag="PKN92_03470"
     CDS             27154..28194
                     /gene="ilvC"
                     /locus_tag="PKN92_03470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNZ47884.1"
                     /translation="MSKAKRVLKNETIAVIGYGVQGPAQSLNLRDNGFNVIVGQAKEF
                     KRDWDRAVKDGWKPGVSLFSIEEACEKATVIQLLVSDGAQKKIWPTIKKLLKPGDALY
                     FSHGFSITYKKLTKVIPPDYVDVILVAPKGSGTSVRRNFLSGAGINSSFAIGQDFTGK
                     AEERAMALGIGIGSGYLFPTTFEKEVFSDLVGERGVLMGALAGIMEAQYEVLRKNGHS
                     PSEAFNETVEELTQSLIRLVDENGMDWMYNNTSATAQRGALDWKPKFKKATLPLFKEL
                     YKRVASGEECKRVLSSTGKKNYQETLQKELEVIRNSEMWRAGAASRALRPKTPQGQVV
                     KSNVGTAGRSHN"
     gene            28372..>28794
                     /locus_tag="PKN92_03475"
     CDS             28372..>28794
                     /locus_tag="PKN92_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47885.1"
                     /translation="MQQEKIEALLGPIDGHYFKGQYSDPEISIRLLSDLGKKRQNNED
                     SCMLYVPGSEALTDSRGILVAVADGMGGAKAGEYASHNALRCICPVYYERNPHALVPA
                     ALRQAVEAANAFVFRESEHNIDFSGMGTKVSALALVGNW"
BASE COUNT         7443 a         6706 c         7191 g         7454 t
ORIGIN      
        1 tgttctgaag tgatttcatc ggtgaaagga ttaatcgcaa caactttgta agcactgaca
       61 tctacgggaa atgacatttc aaaacttcct tgattattaa tgtttttggg gatcaacagg
      121 cagaacgcac aggaaagagt attacagaca taccctttaa tcagtatatc acgaggaggg
      181 gaagtgtttc accccgattt cttgattggc atgtgggatt tcaggtatac atacctgatt
      241 aggttttcat tgcgcttctt ttcgacctgg taaccaatca ttttttaatt caggagaaat
      301 catgatgggc gggttcacaa aaaagtcttg ttctttgctc cttctcgtcg tttcagcgat
      361 ttttgcctcg ctgctgcacg cggacgtgct tcccagaagc gaacatccgg agccgctcat
      421 gttcaggtcg gattggatga cgctcaacgg agtgtgggaa tttgccgaaa cagacggcaa
      481 tgaaacttac ttggatagcc gcctgtatcc cgatcagatt acagtgcctt tttgccggga
      541 gagtgctttg tccgggttgg cccgcaccca atttgtaaaa aatgtctggt atcgcaaaat
      601 ggtttccttg ccgccatcct ggcagggcaa gcaaattttt ctgcatatcg gtgcctgcga
      661 tttcaggaca accctttatg tcaacaataa agaagcctat gtgcatcagg gcggcagttc
      721 ccccatccgc gctgatataa cggcatttct tcaatcgggc gaaaacacac tgatcgttca
      781 tgcttttgat gatgtacgaa gcggcttgca ggcaggcggc aagcaaagtc cccagccgga
      841 aagctatggc tgcatgtata cccgaactac aggtatttgg cagagtgtct ggcttgaggc
      901 cgtggatccc cttcacatag tgtcctatcg ggtggcggca gatcgcggtc ggggcggcgc
      961 tttactcgac gttaatctat cgggcgtcgc agaaggtctt aaacttactg tccgtgcctt
     1021 ttccgacgaa gtacccataa gcgaagcgac cgtgaggtgc gcaggcagca gcacttccct
     1081 cttcctctct atcccggcac ctcgctggtg gatgcctgaa gatccttttc tttatgatct
     1141 cgaattacgt cttgaagagg gagatcgggt atgcgacata gtgtccggtt atttcggcat
     1201 gcgcaccgtt gagctgaagg gacggtcttt tctgctgaac ggggaagcaa ttttccagag
     1261 actggttctt gatcagggct tttatcccga cggtatctgg acagcaccct cggatgaggc
     1321 actcaagaaa gatatagagc tttccatggc ggccgggttc aatggtgcaa gactgcatca
     1381 gaaagtattt gaaccccgtt ttctttattg ggccgaccgt ctgggctata tggtctgggg
     1441 cgagtatccc aattggggat tggagcccgg aaatccggca tctttcgaca cagttctggc
     1501 ggagtgggct gcaataatag ccagagattt taaccacccg tccattatcg gatggtgtcc
     1561 tttcaatgaa accacagcaa aagatattcc tttgcaggcg atggttgtgg atcttacaaa
     1621 aaggctggat ccctttcgcc ctgtgctgga aacaagcgga tatacgcatg gcgttgctga
     1681 tccccaactt ttggatgccc atgattatga tcagaatcct gaaactttta gaaaacgctg
     1741 gacagcggcg ctttgctcgc aggaactacc gcggcgctat ggcggtcttt ctgtagaagt
     1801 tccttttttc gtaagcgaat tcggaggcat tggctggaat gtgaataaag gggcgtgggg
     1861 ctatggaaat acgcctcaga gtctggaaga attttatgaa cggtacaacg gtctcacagc
     1921 ggcattgctt gataaccggt actgcttcgc tttctgttat acccagctga ccgacgtaga
     1981 acaggagcaa aacgggattt acgattatca ccggaaccct aaatttgatc ttgcgccggt
     2041 aataaaagca aatacgcgaa aggctgccta tgaatgcgac ccgccgctgg gcctgccgtc
     2101 agacagtgac gcgatgcagg tcctggttgg cgctgccccg gacggtgatt gcgcaagcgc
     2161 atggaaatat acgatggaaa agccggcgaa ggactggatg acggtatctt tcaatgatca
     2221 cgcctggaaa acaggggctg gagccttcgg caaaaaagag ggcagcgaac atttcatccg
     2281 gactctctgg gatacggagc agatctggct gcgaaaaagt ttttcctgca cacatgatga
     2341 actggataaa gcttttctgg ttatgcacca tgatgatgat gttgaagtgt atctgaatgg
     2401 gaaacctcta atttctttag atcgctggaa tgacagatac gaggcttttg atgtgactga
     2461 ggcagtctgt tccgccatga aaaaaggcgc aaatatgctt gccattcacg ggcgtcagca
     2521 ccacggcggc ggtcagttta ttgatgcggc acttctggtg tcaccaaaaa tgaagaaagc
     2581 ggatgagatg aatattgaaa aaaggacatt cggcactacg gcttccggtg aaagtgtgga
     2641 tatctatacc ctgactgcag cagaagactt caaggtggaa atcaccaatt ttggcggtat
     2701 tattgtctct attcagacgc cggacagaaa cggaagtctc gctgatattg tgctgggctt
     2761 tgacagtctg gataaatatg aagggaaaca tccttatttt ggcgccattg tggggcgcta
     2821 tggaaacagg attgccaacg gacgcttcga tctggacggc aggacgtaca cattggcaca
     2881 gaatgacgga gaaaatcatc ttcacggtgg cattataggt ttcgacaaag cggtgtggaa
     2941 agcggaaacg ctgcgccgcg aagatgcggt gggactggtg ctgacacatc gcagcccgga
     3001 cggagatgaa ggatatcccg gcaatctgga ttgtacagtg acttattggg ttacgagaga
     3061 taaaacgctg gagattgaat acgaagctgt cacagacaaa gcgactcccg tgaatctgac
     3121 caatcacagt tatttcaatc ttgacggaca cgactccggc gctattctaa accagactct
     3181 cgctatctat gccgagtaca tgacccccgt cgattcgact ttgatcccga caggtgaatt
     3241 gcgccctgtt gcagggacgc cactcgactt ccgtactcca gtggcgattg gtgcccatat
     3301 tgatacagcg gatgaacaac tgcgtttcgg aaaagggtac gatcacaatt ttgtcattga
     3361 gaaggatccg caaggacgga tgaagcgggc agcccgtgcg cattcagccc ggagcgggcg
     3421 tgtacttgaa tgctggacca cagagccggg tgttcaattt tattgcggca atttcctgga
     3481 cgggtccgac gtcggtaaag gcggtgttgt ttatcatcat cgcaatgctt tctgtctgga
     3541 aacacagcat tttccaaatt cgcccaatca gccttccttt cccaccacta tccttcgccc
     3601 gggcgaaaag tacgacagca aaacagtgta tcgtttttcc gctgaataac gtttataagt
     3661 aagaataagt taaaaatgcc ctggtgattt gacgcatatc agggatggag ttggaaatgt
     3721 ctgattgtaa gtcaccttta ccacgccaaa aagtcctcga agagcacacc tggaatttaa
     3781 agccgctttt taaaagcgat gctgcctggg aaaaagcctt taaaaaggcg gagtccgaac
     3841 ttccggcaat cggcgtattt aaagggaaac tgtccaaatc cgcagcaaag ttgcttgcct
     3901 gttgccgctt gtccacttcc attcacctta atcttgaaaa gattggtgct tatgcgcagc
     3961 tgaagtatgc tgaagacata accaatccgc tttatcaggg catggttgcc cgcttttccc
     4021 ggcttgccat gcttgccagc gaagcggaga gttatatcaa cccggaaatt caggctattc
     4081 cccgaaaaat aatggatggc tatttgaaag atccgattct ggcggaatac aaagctcttt
     4141 taaaagatct gctgcgctac cgtcctcatg tgctgacccc tgcagaagaa cggcttcttg
     4201 ccatgcaggg cgaagtaagc gaagcgcctt ccaaaatatt tgatcagctt acagacggcg
     4261 atcttgtgtt tgggaccgtg acagatgaaa acgggaaaga aagcgcgctg acccaaagtt
     4321 cgttcgtcac ttttcttgag tcgcccaaaa gaagtgtgcg caaagaagcc tttgaaaagt
     4381 actatcaggt ttttgaatcc cacaaataca gtctggcttc tatcttgaac gcctctttgc
     4441 tgcaggatat ttactatgcc cgggcccgga atttcccgtc tgctcgtgag tcggcattgt
     4501 ttgcagatcg ccttcctgtg tctgtatatg actcactgat tacggccgtt cgagacgggc
     4561 ttccttctgt gcaccgttat tacgccctcc gtaagcgggt tctgcgactt aaagacattc
     4621 atttttatga tacctatcag cctctggtga aagggatgag tgtttccatt ccgtatgaag
     4681 aggcagttga aaccatttgt gatgctatgg gcgttctggg taatgattat gtcggtgcgc
     4741 ttcgcaaagg actgactgct gaccggtggg ttgaccggta tgaaaatgca ggcaaaatga
     4801 gcggtgcctt ttcttatggt gtctacggat gcccgcccta tatccttatg aactatgagg
     4861 acaaagttct gaacagtatg tttactctgg cgcatgaagc aggacattcc atgcattctt
     4921 attacagtat gcgccgtcag cctccccaat acgcatccta ccctatcttt cttgctgaaa
     4981 ttgcttcaac gtttaatgag caggttttgg gtcatcgctt gctggaaaag gcgaaacaca
     5041 aaaaggagcg cactttttta atcaacaagc aaatcgatga aatccggggt accattattc
     5101 gccagacgat gttcgcagaa tttgaaaaga ggatccacga acaggctgaa gctggtattc
     5161 ctatgacaga ggaaagtttc cgtcagatct acagagatct attaaacgat tatttcggga
     5221 ccggttttgc cattgatgat tctttggaac tcgaatgctt cagaatcccg catttttacc
     5281 gggctttcta tgtttttaaa tatgcaactg gcctgtcagc agctattgcg ttggcccaac
     5341 aagttcttgg cggcggcagc agagaaagag aacaatacct gcaatttctg agcgcaggcg
     5401 gttcgcagcc acccctcgtt acattgaaga aagcaggcgt cgatcttacc ggaccggatg
     5461 ccgtttgtga tgccatgaca aaattcagca acctcgtgga tgaactggaa agtcttctgt
     5521 aacacctatc ctataaccct ataaaggaat accactcaac atgaaacgta ccatttcagt
     5581 gcagtcaggt gtttacaggt gcgcattatt gttatctact ctgctgcttc ccttagcggg
     5641 catggctttg gagccggaac aaagacaggc cgcacagtct caactgtcac tgctgacccg
     5701 ggcactggat ttcgaagcac agcgggcaag cagcagcaat aaagatttgg ggcgcaacgg
     5761 agatgcgcac tctatcctgc cgggggaaag cctgacgctg ttggatgcgg ctggtcccgg
     5821 aattgtaaat catttctgga ataccgtggg cacagcagac cctttctacg cgcgttcgct
     5881 cgtactgcgt atttattacg acggacagga gtcaccctct gtggaagcgc ctctgggaga
     5941 ctttttcggt gtggggcatg gtgccgcaaa agaactggac tccattcctg tgtctgtctc
     6001 gtctcttggg cgttccaggg tgtgttactg ggcgatgcct ttcagaaaac acatccgtgt
     6061 tgttgtagca aatgaaaatc ctgcagtaaa agtagattca ttttattatt atcttgactg
     6121 gcaaaaagtc gatcagcttc ctgaggatac gctttatttt cacgcccgtt acaggcagga
     6181 ggcgcctgca tcagagggca attatacaat ccttcaaacg gaagggcgtg gccattatgt
     6241 tggcactgtt ttgtccgtgc atcagatgga aacaggctgg tttggcgaag gcgatgattt
     6301 tttttacata gaccatgctg aaacgccgca gcttaagggc acgggtacgg aagattattt
     6361 taatgatgcc tggggcttca gggagttttg tcgtccctat cacggagtgt ctctttacga
     6421 aggcgttgtt gttggggacc gtgttactgc ctatcgttgg cacatccttg atccggttcc
     6481 gtttaaagag tcactgcggc tcgagattga gcataaaggc agtatttttg atgaagaggc
     6541 gcccattgca aaatttgaaa ttggagggtt tatagagcgt tcagattggg tcagttcagt
     6601 tgccttctgg taccagtatc cgccggtaaa cagtgacgag ccctttcccg catgggcgga
     6661 tcggatgcct ccctataaaa ttatttcggc aagtgacctc tcctataaag cggatcctcc
     6721 cctgcttgta ttgcctttgt caggtgccat tctatatgct cccatgtctg gtaccggcga
     6781 aatcacctat acgtttgcga ttgaggaaaa agggacctat cgtcttgacg ggttattcac
     6841 ccattcgctc atgtgcggcg tatatcagcc ctatcttgat gatgcgccca tcggtgcacc
     6901 agtagatttc agtgcgccgg gttttgatcc gctctggatg cacctggata cgctcatact
     6961 tgatgccggg gaacatcatc tgaaatttgt cggaacagga gcgacacccc gtttttatcg
     7021 gtcaatggct cccttgcaca atggtatagg catcctgtcg ctgagtgtgc tgcatttgga
     7081 ggaaatggca ggatttaaag tcgctttgaa aaaagagcag gataaaaaag aattgccgca
     7141 tgatcaaaat actggcagat gaaaacattc cttatggaat agatgctttc agcactttag
     7201 ggactgtgca attagtcgct gggcgggcta tttcacagga aatgcttaaa gataaagatg
     7261 cgcttattgt tcgcagcatt acaaaggtta acaaagagct gctgtcgggt actgccgtcc
     7321 gctttgtcgg aacgtgcacc atcggcgaag atcatgtgga caagcagtat ctggaagagc
     7381 aaggcattgc tttttccagt gcaccgggct gcaatgccga cagcgtggca gattatatta
     7441 ccggggcact tttgctgcta gagaaccact tttcacttgc cctgtcccaa atgacacttg
     7501 gagtggtggg cgtaggtaat gtcggatcac gggtggtcaa acgggcgcaa gccctcggca
     7561 tgacctgtat cctcaacgat ccgccgctgg cagaaaagaa cggaaacagc ggctatgctt
     7621 cgctggaaga aatagtaaag tgtgacatca taacctttca cacccctctg atcaaagaag
     7681 ggaaatggcc tacacggcat ctggtgaatg aggcatttat cgcttccctc aagcctgaaa
     7741 ccatcataat caacacagcg cggggtgctg ttgccgataa tgcggcgctt aaaagcgcac
     7801 tgcgcagtaa tagaatcaga gctgctgttc tggatgtttg ggaaaatgag ccctctattg
     7861 atccggagct actggcgctg acgtttttag gtacccctca tattgcaggc tattcctttg
     7921 acggaaaagt gaacggaacg acacagatct atgcgtctct gtgctctttt cttggcgtag
     7981 atgcgcagtg gaaagccgat gctgttatgc cgcctccttt atgtcctgaa gtaacggttg
     8041 atccggacga tccgaactgc cttctgcatg ccgtttccaa agtgtatgat ttcaaagaag
     8101 acgacaggcg gatgcgtgaa ttatgttctt tgccgcccga taaaattggc gttcactttg
     8161 atttgctgcg caaacaatat cctgtaaggc gtgagttcca taacaccagc gccttgctca
     8221 cacggcagga ctacgccctg acatcgtctt tatccggcat cggctttact gtaacatgat
     8281 agcgggtcat tccctgctat cccgccacca acgggcgcac tgcaggaacc gacgtgagat
     8341 agaacgcttt tttcacaaga gaggcttctt acatggttaa aaatataata aaacaggacg
     8401 gctatctcac taatgaagag atagcgtctt ttgttcagga aacactgtcc gatttcccgc
     8461 tgacagggaa acgagtgctt tttattatcc cggatcagac ccgcagtatg cccatgcctg
     8521 ttatgtttcg tgcgcttttt gaagcagtca acggacgtgc cgctaaaatg gatttcctga
     8581 ttgctctcgg tacgcatccg cccatgtcca acacgatgat tgagaagttg ctgggaatat
     8641 cagatgaaga acgcaaaggc atttacaaag atatcgggat tttcaatcac gaatggcaaa
     8701 accccgatgc ccttacctgt ctgggtgaaa tcagcgcaga ggagatcggc actttatcag
     8761 gcggattatt gtttcaatcg gcaaaagtga ccattaacaa aagaattctg gattatgacc
     8821 accttatagt cgtaggaccg gtctttcccc atgaagttgt tggtttcagc ggcggaaata
     8881 aatattttta tcccggtatt tccggtgcgg aaatcctgaa tctttttcac tggctgggcg
     8941 ccctgctcac caactatgct gtcaacggaa caaagcatac agcagtacgg gcagtcgtcg
     9001 accgggcagc ttccatgatt ccggttgaaa aacactgttt ttgcctgaat gtgtccggca
     9061 cagccacgaa agcgatttac tttggcagcc cggaagaagc ctggagcgaa gcggctgatt
     9121 taagtgcaca gacgcatgtt atctacaaag ataagccttt tcaaagtatt ctagccatgg
     9181 cgcctcccat gtatgatgag ctttgggtgg caggaaaatg catgtataaa ctggagccgg
     9241 tagttgccga tggcggtgaa ttaattattt tcggaaaaca cattcgtgaa gtcagtgtta
     9301 cccacggcga acttattgcc cgtatcgggt atcacgttcg ggattatttc gttaaacaac
     9361 cggataaatt cagagacatc cccggaggta ttctggcgca ttccacccat gtccgcggca
     9421 tgggaagttt tgaaaacggc attgaaaagc cgcgggttca ggttaccctt gccacatcca
     9481 ttccggaggc ggaatgcctt gccatcaatc tgggctatcg cgatcccgat tccattactc
     9541 ctgaagactg gcgggatcag gaagatcagg ggaaattgct tgttccaaat gcaggcgaag
     9601 tgctttatcg cctgacttct tacgtttaat gctcttccca tcaacaattt ttggagtaaa
     9661 cggttcatga agaaaaatgt actagttgcc cagtccggcg gtcccagccc cgtaattaac
     9721 agttcactgc gtggtgtaat tgatacctgc aagatgtttc ctgatatttt cggaactgtt
     9781 tatgccggct ggcacggcat agaaggcgtt ttgaaagaag aattgctgaa cctcagcgcg
     9841 cagacagacg aagaagtcgc tctgctgcga tataccccgg cggccggcgc tatcggcaca
     9901 tgtcgctata aactgaagtc ttcccaaaag gaagactttc aaagggtgct tgatgtttta
     9961 aaagcccaca atatcggcta tttcttttat aacggcggca atgattccat ggacacggcg
    10021 aacaaagtgg caaagctcgc catcgaacag gggcttgacc ttgttgcagt cggcgttccg
    10081 aaaacaatcg ataatgatgt cggtgattcg gaatttaaac tgattgacca tacgccggga
    10141 tacggaagtg ttgccaggta ttggatgtcc atggtgcaaa atgccaatga agaaaatgcc
    10201 ggatcatctc ctgctgaccc tgttctcgta ttgcaggcta tgggcaggcg tataggttac
    10261 attcctgccg ctgcgcgcct tgctgatccg aaacgtgaaa tgcctctgca gatttatatg
    10321 gcagaatcga aaattagtgc gcaggaattg tgcgatcggg taaacgacca gatccgaaaa
    10381 gacggacgct gtctggttgt tgtcagcgaa ggttttgatg tgggagagct gggtgaggtg
    10441 aaggacagtt tcggtcatac cagtttcggc tcttcgcaga tgacagtggc tcagaaagtg
    10501 gtgaatcttt tgaataaaca tggattgccc gctgcaggaa gcgcccgcgg tcaggtgccc
    10561 ggaaccgatc agcgtgctac aaccgcctat gcctccattg tcgatctgga tgaggcatat
    10621 aaagtgggac agcaggctgt tctgattgct gtttcgggag aaaatggctg gatgtcgaca
    10681 attctccgcg agccgggtcc catttacaac gttcgttatg acaaggtgcc tcttgagtct
    10741 gtagccaact cggaacgcag tttccccgat gcctggattt cagaatcgcg aactgacgtt
    10801 acggatgcat tcgtggcata tgcaaagccg ctcatcggag aagactggcc cagcgttccc
    10861 gtgattaacg gaaggcagcg tctttgccgc tttgagccga ttttcgccga taaaaaactg
    10921 cccccttata cacttgaagc gttgcggtag ctgcggtttt ttacagaggg aaagtaaaac
    10981 gggatccgcc gttgcattgg acaaatatat tcggaaaggc agccgccaga gcttcgattg
    11041 ctttttctcc ggtgcagtgg cagggtgcga gcattgccag atcaaagcgt ttcagagcat
    11101 caatggttgt gagcagacga tgctctgaag cgttttttaa gtggaatccg ccgataacag
    11161 cacaaatctt ccggggaagt atcagcgttt gaatataccg gagcgtattg atcactcctg
    11221 cgtgtgcgca gccgagcagg acaattacgc cgcaggatgc attaatccaa agcgcctgat
    11281 catcagcaat actgtcctcg agcgtacact cccggtcaag ataaaaaggc cccccggtat
    11341 cttcgaaaga tgcagtacgg ggaatagccc cggtgacccg gatatttttc aaaacttcaa
    11401 caggttggga tgtatgaaca gcacggaaat ccggatgctg aagcaggttg aacgaactgc
    11461 aaggaatgcc aatagatttt gctgttccat cgtccatacg gcggtatctg tcttgaagcg
    11521 cttccggatg aaaataaatc ggtagatttc gaaaaagtgc agcaccgtat gccagtcccc
    11581 ctgtatggtc ataatgccca tgactcagta ccagggcgct gagtttgtcc aaagatattt
    11641 gcagggtttt agcattgtca agaaaaacat cgctctgccc tgtatcaaac agcagattgc
    11701 cggacggcgt tgatacatgc agagacaaac cgtgttccgc tcggatatga ccaccgatgc
    11761 tgtcattgtt gcagaggact gtaatatcaa cgctggagcg cattgcattt tcctcagggt
    11821 tcgaagtggg tgatggttga gtttcggatt aactctcttc ggtcagtgcc tgcacaattt
    11881 tttcaaaagc ctttgcgccc gccgaagcgg aaaaagcctg agtatacgga gtcccgtcat
    11941 cgccggcacg tcccatgtct ggatcaatgg ggatgctgcc caggaaagga acgcccatgt
    12001 ctttcgccat gcgctctccc ccgcctgatt taaaaatatc gtgccgctca ccgcagcccg
    12061 ggcagataaa gccgctcata ttttccacta caccaaggac gggcattttc atctgcttgc
    12121 aaaaattaat tgacttgcgc acatcagcga cagacacatc ctgcggagtg gtaacaatga
    12181 cagcgccatc cgcatcaggc aaatattgac agacggacaa aggctcatcg cccgttcccg
    12241 gcggggaatc aacgatcaga tagtccagtt caccccattc cacatctttt aaaaactgtt
    12301 taatgactcc tattttcatg gggccccgcc agatgacagc atcatcggca ttgcgcagaa
    12361 aaaagccgat tgacacaacc ttcatgtccc ctacttccac aggtacaatt gccccttcgt
    12421 tgttttgaac cgtcatgcct tccagtccga gcatttttgg tacgctggga ccgtggatat
    12481 ccacatcgag caagccgacc cgtttccccg caagagacag agatgcacag agattaacag
    12541 ccaccgtact cttgccgacg ccacctttgc cggacagaac cagtattttg tgttttattt
    12601 gacacatgcg ctccatcagc agctgcctgt ctgtaaattc ctgttctgac tcatggctta
    12661 agcgggattt ggctgcacag tcctgtttgc tgcagctgtt gcagtcactg gcggtgtgtg
    12721 acgttttctg gttcatgaga acttctccaa aaaggacgta aaaggctgtt gtgtttttaa
    12781 aaaaggggca ccatcactac gattagaagt gaataaaccc tatcatatat aacgggaaaa
    12841 caacaaaatg aattccaaag gcttcaggaa tgattcgttt tgtctgtttg tcatttgata
    12901 tgattactgt gtcttttact ggcggtcgcg actgtttccc ggcgactgaa tatggtcaca
    12961 ataaaaggaa aaaacaaaat gaaacaaatg gatatcggac tgatcggtct tgcagtaatg
    13021 ggcgaaaatc ttgttttaaa catggaaagc aaaggcttta gtgttgccgt ttttaaccga
    13081 acagtcgaaa aagtggatgc ttttattcag gggcgcggtg ccggaaagaa ttttctgggc
    13141 tgccacagta ttgcggaact atgtgcaagc ctgaaacgcc cgagaaaagt gatgatgctg
    13201 gttaaagcag gtgtggcagt ggatgatttt attgaacaga taattccgca tctggaagaa
    13261 ggcgatatta ttattgacgg cggaaacagc cattttcctg acaccattcg ccgcacagcc
    13321 tacgttgaaa gcaaggggct gcgctatatc ggtaccggcg tgtccggcgg agaagaagga
    13381 gcgttgctgg gtccgtccat gatgccgggc ggaacaccca gcgcctgggc tgaagtcaaa
    13441 gagattttcc aaaaaatatg tgcgcaaact gatgcaggtg aaccatgctg tgattgggtg
    13501 ggtgaaggtg gagccgggca tttcgttaaa atggtgcata acggcattga atacggtgat
    13561 atgcagatga tttgtgagac ctatcatctg atgcattccg gtcttgggat gtctaacagt
    13621 gaaatgcatg aagtctttgc cgaatggaat cgaggtgaat tggactccta cctcatcgaa
    13681 attacacggg atattctcgg gtataaagac gaggacggca acgaagttgt ggatctgatc
    13741 ctggacacgg ctggacagaa agggacagga aaatggaccg ttgtcgccgc acttgatgaa
    13801 ggtcagcccc tgacgctgat cggcgaagct gtttttgccc ggtgcctttc cgccattaaa
    13861 gaagagcgtg tagaagcatc caagctgctg ccgggactgg caaagacttt tgacggtgac
    13921 aaactcgcct tcatcgagga tttgcgtaaa gcactttatg cggcgaaaat tatttcctat
    13981 gcgcagggat atcagctgat gcgtgctgct gccgaaagct atggctggca tctcaattat
    14041 ggcggtattg ccctgatgtg gcgaggaggc tgaattatcc gttctgtttt tctgggaaaa
    14101 attaaggagg cctttgataa aaatcctgaa ctgaaaaacc ttctgctcga tcctttcttt
    14161 gcggaaattg tgaaagagag ccaggctgcc tggcgtcggg ttattgtcaa agcgaccgaa
    14221 atgggaatac ccactcctgc cctgtcttca gctcttgctt tctttgacgg atacagaagt
    14281 gaacgcctgc ctgccaatct gctgcaggca cagcgcgatt attttggcgc tcatacctac
    14341 gagcgggtcg acaaagcacg cggtgaattt ttccatacca actggacggg acgaggcggc
    14401 gatactgcag ccagcaccta tatcgtgtaa ttttctttgc caagataaaa taaaacggca
    14461 ggacaggctg aaacgtctgt cctgccgtac aatttcagaa aaaacgattg gtttaaatta
    14521 agccaccgac ttttagggag tattcgggtc gaagagggct tcaaaaatgg aaatgggata
    14581 ccataaaagc ccggcaataa cgccaattac atacgcaaag gggtaaagga tggttgtctg
    14641 cagggcgaaa aacagacctt tattttccat gatcatctct cctttaagta aagctgcatt
    14701 tttcaaagaa acttttttgc ttgctcttcc tgatcatttt aaacttaatt gaggaattca
    14761 gtcaagcagg cggcgaagtg aacccgacgg cttgcaaaaa aacatagaga tctgttgtac
    14821 tgcaggcttc tcttttaaaa atggtatact ctgtaaattc aatcaggcaa tactgccggt
    14881 gttttgtatt cttcaacagt tttaaaacag gttttcttta aaaggttcgc aatttgctga
    14941 tctgtcttga tcagcatttt taagcaaagg acttctttat gcgcactaat tgctttacca
    15001 aactcgtctg tacgatcggg cctgcttctg aatctcctga ggtaatcacg gaaatgctcc
    15061 tggcaggcat gagtgttgca cggcttaatt tttcgcatgg cgaattcgac tggcacggga
    15121 caatgatccg tcgccttcgt gaatgtgcgg caaaagtcgg gaggtctctt gccattcttg
    15181 cggatctgcc gggggtgaaa atccgtgtcg gctgtatgaa agaagaaggg gttcagatcg
    15241 tcgaaggttc tacgctgatt ttaacaacgg aagaggtgga aggcgatacc aaacggattc
    15301 aaatcagttt gaaaaaactg ccgtcaacct taaagcccgg tgatcaggtg ttcatcaatg
    15361 acgggattat tcatcttgaa ataatggaaa tcgaaggcaa tgaggcacgg tgccgggttc
    15421 tggtaggcgg cgagctcaga tcccgaaaag gaatcaatct tccgggtatt gatttagata
    15481 ttgatgcaat aacggaacag gatcttaagt gcctgaaatt tgctctgaat cagggtgtgg
    15541 atgcaatcgg tcagtctttt gttaccagcg cgaaagatgt cgaatgcctg cgcaacgctg
    15601 ctgctgccat gggcttctcc ccttttatta ttgcaaaaat tgagaggagt caggcgctgg
    15661 aagccatgga agccattatg tatgctgctg atggcattat gatcggtcgc ggtgatttgg
    15721 gcgtggaaat tccgttggag acgattgccc tggctcagaa agaactgatc tacagagcaa
    15781 atcttctggg caagcccatc attacagcta cccatatgtt ggattcaatg acgtgcagat
    15841 tgcggcccac aagggcagaa gtgtccgacg ttgctaatgc aattctggat ggaacggatg
    15901 cggttatgct ttcggaagaa tccgctacgg gctcttaccc cgttgaagca gtcacaatgc
    15961 tttcaaaaat tgctgtcgca acagaggcga gcagaccgaa agagtgtccc tgggagcatc
    16021 tgatagacag gggcgtacga aaagataccg cttttgttga ttatatctcg cgcaacgtaa
    16081 accatgccgt tgatcatttg cagcccgacg ctgtagtggt acctacttct tcaggctata
    16141 cagcaaggat ggtttcacgt tttaaactac ctctctggat tgcggcagtc accccttctc
    16201 cgcgtgtgtc gcaaggcttg attttctcct atggtgtgga gcctgtgctg acgcaggaaa
    16261 gcatcaccga ctggacggca ttctcaaaag agtggataaa gtctttgaat gcgcaaaaaa
    16321 atggtttagg gattctgcta caggggcctt ctccgtcgca ccccgaaacc aatccgtcca
    16381 tggagattat tttacctgat aaaggataat caagtcgttt gaccccgcag tagcccgtgg
    16441 ttagcagccg aagagaagcc atttatcttc ggcttttttt tctgcaataa atgcgcattt
    16501 taatttaaat agttctgttt ctatagtgtt aataatatgt acagtgttta gacggaaaga
    16561 aaaagcttga ttttttacag ggtatttgct aaactgatta ttcagatgga gtattttttc
    16621 attatatagt aaacggttac tgtcggccag gtcagccagg aattggaggc ccctgaagtc
    16681 cctgcttcct tttgattgtt caaacaactc tggaaagcac ttttaccaga gttggaaacc
    16741 cagaaaagga gaaagaaaat gtttaagaaa cataaagggt ttaccctgat tgaactgctt
    16801 gtcgtcatcg ccatcattgg tattcttgct gccattcttc tgcctgctct tgcccgtgct
    16861 agagaagccg cccgccgttc cagttgccaa aacaacctga aacagtgggg tattgtcttc
    16921 aaaatgtatt ccggggaatc tccgggagaa aaatatccgc ccatcactgc ccactatatg
    16981 ccactgggtc ttgtggactg caatcttgaa ggcggtacac agcgcaattt tgtgattgcc
    17041 gccggcgtgt acatcggcgc tgtttatcct gaatacctga cggatcctgg cataaacttc
    17101 tgcccctccg acccgaacaa taaacctact gaagcttatg caacggtcga tttccccaat
    17161 ccgctgaccg gacagactgt gcgcaagggg gatcctattt tcggcatacc gtgcatgcaa
    17221 atgacaaaag ggatgcagaa ctctgacgaa agttactggt acacaggctt tgttttcgat
    17281 atgcttgagg atactttcaa caccggtatt caggcttttg ccggaccccg ccagatgctt
    17341 gaggctgcca tacaacttga cctcctcggg ctcatcaccg gttcttcctc accgcaaacc
    17401 gttgagtcac gtgttgacaa agatgtgaag gtgacgcctg gctttggtaa tggcggcagc
    17461 tacaatgatc ccctcaacgg cagagacacc gtttatcgct tccgtgaggg tatcgagcgg
    17521 tttatgatca cggacatcaa caatcccgca gccagtgcca tggcgcagag cactttgttt
    17581 attatggcgg atggtgtttc tacgaatctg tctgaattta accatgtgcc cggcggaagc
    17641 aatgtccttt atatggacgg gcacgttgag ttccgccgtt acgaggaaaa aggacagggt
    17701 cctgtaaatg ggcctgtcgc ccgctttgtt ggctatctga tgtccggtgc taacttaagc
    17761 atctgatacg gaaacccttt ttgtctgtca gtttcaagac cctctttgat tttccaaaga
    17821 gggtctgttt ttgcaatact attcgtgtag cccgggcagt tacacttgtt aaaaattctg
    17881 agtgaattta cattcaaatg gagtatcacc tatgcctact tccacttttg cctgcatgat
    17941 caattgcatg gacgggaggg ttcaggaagc tgtcattcgc catttaaaag cgaggttagg
    18001 agtggatttt gttgatgccg ttacagaacc ggggccgatt gcccatcttt caaaaggggc
    18061 ttcggtgtgg gttgataaca ttaaggcttg cacagacgtt tccgttgaaa aacacaatgc
    18121 gaaagctttg gccatagtcg ggcattttga ctgtgccggc aatccggttt ctaaggaaga
    18181 acaagtgaag cagctgcacc aatctgtgca ggttttaaaa gaatggtatc cgcaccttcc
    18241 catttatgca ttgtgggtca atgaacaatt ggaagtggaa gaaatagttt cataaaaaga
    18301 gaaaaaattt aaggacaccg gtgtagaaaa caagccctgt ctcatgcctt caacatgttc
    18361 atggtcatga tggaaaccgc tgattcattc ttattcagat tctgaagccg gtatagatct
    18421 ttgttaagca atccgaaaga gacacacccg atattcgcgc ataagcatta tattttatta
    18481 gcctgcattc ttctggcggg gcttgccatt cgcatcgctt atcacagcga acatgtacat
    18541 gcgccggact atgcctctcc tgtccttgat ccgcaactga atgattattg ggcacgggct
    18601 cttgtttcag gagattggac tcccccgcct catgcggatg atccaaaaat taggaccacc
    18661 ccctatgggc ggccgcccgg gtatccctgg attcttgccg gtatttacac gctctttcag
    18721 gaaagttatc acggcccgcg tatcgttcag accgtggccg gtttgtgtgt aattctcctt
    18781 ttatgtgcga cagcacactc acttttcaac gctgaagttg cgctgttgtc ggctgcgctc
    18841 tttgcgtttt attggggcag tccttatttt gaaggagaac tcaattctcc tatctgggaa
    18901 ctcctttttc ttctggcagt cttctttttg tttttaaaat attgcaaaac cagcaaaaaa
    18961 tcttacatgt ttttatcggg cctgctgggt ggaatttcac tgctcattag gcccaacttg
    19021 cttgtcgtac tgttcttcat gattggctat ctgcttgtta cggcaggtgt agccaggcag
    19081 aaattgcaga cattactttt aaatgcgggt ctttttgttt ttgccgttgc tatcccgatc
    19141 tctcctgttc ttgtgcgtaa ctggcaggtc tccggcgatt ttgtttttat ttcctattat
    19201 gggggtgtga atgcttatat tggaaacaac ccgaaagcgg actgcgtctc ccctacaatt
    19261 cccgatttat atgaaacagc aggtgtagac gcatggaatt gttttaatta tccccagttg
    19321 gttgaaggct tgggccgccg gatgaacaaa tcgtcgcttt ccttttctca ggcatccacc
    19381 tatttttata aaaaagcgct ggatttctgg cggcaagaac cacagcaagc cctttggctg
    19441 acgctgaaaa aagcctggta cttctggagt cctcacatca tctcagacag taaagtcgtg
    19501 tattatgagc gaaaatcatc gccgattctg gcgtgcctgc ccgggtttac tctcatcagc
    19561 gccctggcgt tcacgggcat gatgtttttt cctcttgctg agtggcgcaa aaaaaggtcg
    19621 ctgcacctgc tcctcctttt tggtctttcc tactttctat cgattctccc tttctttatg
    19681 tccgagcgtt atcgttttcc aattatcgtt tttttaatcc cgttttcagc gtatgctgtg
    19741 gcaatcataa aatctgcact tctcggcaag gagtggaagc gatgtttcca tgtcctttcc
    19801 gttttctgcc tgattctggc ggcaatgagt cttcatcttt ttccttataa acctgatgaa
    19861 tctgtctggt atctgcacaa agcctttgcg tatgccggaa ataaaaaaac aaatgaagca
    19921 ctttcctgtc tggaaaaatc gctggcactg aaccctgcca gtggagaagc aatgcttcag
    19981 gccggttata tctatgcgtc gaaaggagaa cacgaacgag cggtcgcact ttatctgaaa
    20041 gcacttgaaa ttaagcctca cagccgttat cttctgaaca atctcgggta tgagcattat
    20101 ctgagcaaag aatatgatcg ggcttgcgac tattacaggc gagcgctgga agtctcgccc
    20161 cgatatacgc tcgcctataa caatcttgga aatgctctgc ttaaactcgg aaattatgaa
    20221 gaggcattag taaattatga cagggcactc gctatagatc cgaaagatcc ttttgctgaa
    20281 tgcaacaggg gcaacctttt tctggaaaca gaacgctatc aggaagcagc agcagcctac
    20341 cgcatggctc tggcggctga ctcgggcaat gcagacgctg ccaataatct tggtttggct
    20401 ttgctccggg acggacaggc agaagattct ctggagtggt tcgaaatggc atggcgctta
    20461 cgtcccccct acccactagc gcacctcaat gcagcaaagg cgtacaggca gctcggtctt
    20521 acggaagagg ccaggcggca ttataaatta tgtctggaat actggcctga aaacgaggaa
    20581 gtgcgtgaag aacttacttt gctgcagggt caggaaaatc ggcagatgga gtagaggcag
    20641 cagcattttc atggacagtc aaagtatcgc cctcggctgc caactgacgc aagcctattt
    20701 tttcgaaaga caggcacacg atcccgatca ggactatggg aaccagatta aagagatggg
    20761 cgacaattgc cacggcgcgt aaactgtcaa gatccagagc gttgttgacg aggataaggc
    20821 caccgacaac accgacgtga aaaggcccaa taaagcccgg cggacctgga atgctgataa
    20881 atacagagag aagagacagg acaacaaaag gtgcaaacca gggcacagac agatcgaagg
    20941 ccttgaaaac tgcatattgg ctgatcgcaa aaaaagtcca cagaagaaga gaaaccaggg
    21001 atgccttgag cagatcttta aaagaaagaa gcacctgcat tccttctgcg aaatttaaaa
    21061 ataatttccg gacaaaatgc cccagtcttt tagaaaacaa agcaaaaaaa gaatccgaaa
    21121 gtgcgagaaa aaatttcttt cgggcaatga gaagcatcaa aaaagcaacg cctgcaatca
    21181 aagcagcagt ggcaacaaaa agcgaaccct gtaccatttc ctttgaaata gctccggaat
    21241 ataatgcttt catatcttca gggagataca catcgctatt cggtctgaag accaggagcg
    21301 tcacaagaaa aacgtaaacc agtccgaaaa gatcagtgag acggtctaca gcgacaaaag
    21361 ccatggtttt aggaaaagga atatcccggc ttttgctgag aaccagggcg cggattactt
    21421 cgccgattct tgcgggaagc acaaaattac ccagaaaccc aatttgggtc gcactaaaga
    21481 gctgccagaa tggcacatga attaccgggt tcacaatgta agaccatcgc cagattcgta
    21541 taacaaagct gattaaaata gcgacaagtc caacaaagag ccaccctgtc cgggcttcct
    21601 gaaaggcacg ccagacttta acccagtcag tgtctttaaa caggaaccag agcaaaaatg
    21661 cgccgatggc gaggcctgct gttatctgta aatatttttt cacgtaagtg catctctttg
    21721 ctgtcggatc ggactgttag aaaaatactt cttcatcaag agaacggtaa tgcaccgctt
    21781 ctgccagatg cttttctgct atcgcttcat ctccgtcaag atcagcgata gtgcgggcga
    21841 cacgaagtac tttatcgtgg gcacgggcac tgagccccat tctgagcacc gctgcttcaa
    21901 gaaatgacat ggcactctct gaaagacggc aatgtttacg cagctgtgat gtgtccatgt
    21961 gcgcattcca ggaattttta ctgccgtttc gggaacgctg gatttcccgg gcagccttta
    22021 cggatgcacg cacttccgca ctgttcggcc cctctttagc cgttgtatgt aattcttcaa
    22081 aacgaagagg agggacatct atatgtatat cgatgcggtc gagcagcggc ccagaaatgc
    22141 gcgacatata tctttgaatt tcgcctacag tgcacttgca ccgcttttgg gggtgggtcc
    22201 ggcatccgca gggacagggg ttcatcgcga cgatcagcat aaaacggctg ggaagtgtga
    22261 tggaataact cgcacgtctt aagtgaactt ctccttcttc aagaggctgg cgaaggactt
    22321 ccagggcgga tctgttgaac tctggaagtt catccagaaa aaggacgcca ttatgggcga
    22381 ggctgacttc acctggaatg ggttggttgg ccccgcctcc agtaatcgat accgtagtag
    22441 ctgtatgatg ggggctgcga aaagggcggt tgagcaccag tttgttacgc atatgaatgc
    22501 cgggtgcgac gctgtaaata cgggtggttt ccagcgcctc ttcaaaggtc aattcaggca
    22561 ggatacccgg aagtcgtgat gccagcatgg ttttgcctgt gccgggcggc cctaccatca
    22621 gcagattatg tgctcccgcc gctgccacag tgagcgcacg tttgacatgg gcctgcccct
    22681 tgacgtccgc aagatccata atacgccgtg cggaatctcg ataaacattt tctaaatcag
    22741 ttacgaaggg cttgatgtcc aactcaccct ttagaaaggc atgggcatta tgcaatgtgt
    22801 caacaggaat aatgtcaatg ttatcaacga caccggcttc ttcagcattt tctactggaa
    22861 tgagaagacc ctttaatcca gtattacggg cggcgatcgc catggcaagg gcaccggtaa
    22921 tgggacggac agaaccgtca agcgccatct ctccggcaat aagatagtct gatattttat
    22981 cggtagtaag ctgccctgtt gcggcaagca agcccagagc aataggcaga tccagagcac
    23041 tcccctgctt gcgcatatct gccggcgcaa ggttaattac agttttgcct tcgggaacct
    23101 tgaaaccgga gtttttaact gccgactgaa cccggtcttc gctttctttc accgctgtgt
    23161 caggaagtcc cacgacctta tagcgaaaaa gacctttttg tgtatgcact tccactgcaa
    23221 cagggtaggc atctattcca acaacagcac tggactgtat aattgctaac aaaaaaggat
    23281 ctccgacggc ttcagcgaac ccggaaacaa accgctcagc ttaaaccaca ggtacacata
    23341 aacaatggat acattgtgta tatattgtcg ggaggtttca agtacagccc cacagatttt
    23401 cagaaaggaa gcgtcacggc agagagacag ggcgtatttc ccaatccacg gtaaaagaat
    23461 gggactcacc cggctgaaga agaactgctt tagcccagag ctcgcattct agatcattga
    23521 tgtactccgg ataagcataa agccaggcat gatccagttg ttcagaatca aaacgctgga
    23581 tcacttccag aggaatatca cctctgaggc accattcatt caacggaatc tcctttttgc
    23641 gaaacttgat accttctcgc agtcccggaa ttacctcgtc tgcctggtgc gtatgcggat
    23701 tgttttccag atcagtgaaa tggacattga attgctgaag ggctcccagg tggaaattgg
    23761 tgtgagaccg catcattgcc tcccggggtg aagcaaggag atttttaacc gtagccttga
    23821 tggttaaaac aggattgtcc ggatgaagtg taaactcccg tgtgataagc cagccgtctg
    23881 cttcagcctg caaggtcagc gcattatcct ttctcgagac aatttcgaac tgataaaaca
    23941 ttcctgcagg atcaaactcc cctcccaatc ggagctcttc tccgccacag aaaggaaaaa
    24001 ataaattccg ggtaatgtta tacccggtca cacacaggcc tgtatttttg tctgttaccc
    24061 taagcgctct gccgcccaga aagggagcaa agtctgcttc cagatacttg ctcgagattt
    24121 taggggcatg aaggggcgaa gaaaaagcaa gtcccaaaag aaaaagctgc tgcggatccc
    24181 cttggacttc tctgattgtt ttgaacccat gtttttcgag cctggagacg aaactggata
    24241 ttgtgtcaaa gtcggcaagg ggttcattaa agattaattg ttcttcttct atcctgtatc
    24301 cattgcgcgg gaaaaaacag gcgtaatgga gcggcatgcg tgctactcgg attttttcca
    24361 gaagattatc atcgctcata gccagcgact ctgcttcgtc aaaaagccga tttcccgcat
    24421 caatgatttc atcgggcaga tagccctgcg ccgggttggt ataaagatgc atatggatgt
    24481 tgtttttgtc cacttcatcc tgaagcatgt ttatataccg gtacacagga ttccatgctt
    24541 ttccgtaata accctgaagg aaatcctgaa ggagctcctg cgcatcataa tccgggttcc
    24601 aaaggagttt catcccgtac cagggacgca gcaggctgaa ttcgcctccc ccgccgctgt
    24661 gtcccatacc ttgcagataa atgccttcaa cgccaatatt tttataaaag cgcatgtctt
    24721 cagccagggc gccaaaattg ggaaaggggc tgtaataatg cacaaaatta acaacatagt
    24781 gccagatata gagatgcttt gtaagttccg cccacctctt tgcacgttcc cgatactgcc
    24841 ggttatgagg gcatgtggct atgctgtgac tgtcacagga aggaaacata tggcacagcc
    24901 atatagcgat attgggatgt agtgccagcc cttcaggagg ctcctccgtg tacatgtatg
    24961 caagcgtccc gatggtcttt tcgggaaaaa agacgctggt acgttcagcc agttgattca
    25021 aaaaccaata ctgggttcct ccggaagtgc catagcgttc gttgatggct gtacattgcg
    25081 ggcactcgca gtaattatag taatccatct gggaaaaatt gtgttgcgtg atttccggtt
    25141 gagctctcat tcgctccagc attttttcag tcactatttc aagtacttcc ggatttgaca
    25201 ggcagagttg tgtttcgtgt tttcttcgta ttcctccaat ttcagaaaaa tattccggat
    25261 ggctgtcaaa gtattctgaa ggggaaataa agcggaaata agtatgacaa cgaccatcct
    25321 gcgtatactg aattccttgc tcccgctcac ctgtgcccat cccgtcgtta tcgcaaaggc
    25381 gtgcaagaaa gagatcatct tttccgggcc aggcatagga cgtatgtcgc cacttaaagg
    25441 caggcttttg aattttatcc atttccggaa gagaaagagt actgatttcg ggaattgttt
    25501 cttcaccggg agcgtaccag cggacactta aaaattcttc aagaaaagcg ttaaccccgt
    25561 aaagagtgcc tgcctcacga ctcccgccta taaaaaaatg gggaggcact gaacgaaaaa
    25621 gaaaaccctg atctccgaca tcctcaaggc tttctgaaat tcccaaacgg gtggctgttt
    25681 cactgaaacc gacaaggagt aacggcatgt tctcttttgc cagatcgctt tgaattaaag
    25741 gcagcagaac accggcagaa gccctgatgc accgctgcaa ttcccgggcg gcaaattttt
    25801 cggacggact cgcttcggag gagacagcga ttgaatagtc gctttgaccg tcatggatca
    25861 gactcagggc ctccgcagaa agggacaggg ggaaaagtga aacaacgcaa aaaacaatga
    25921 tgctgcaggc aacaaggact gattttatgg tctccatatc agattctcct gctttaacag
    25981 accgttattg caggagaaca gtttctttct ccatttttcc gggtttttta ttgagtgcag
    26041 taagctcccg gatgccgtga tcaatcagat actgtcgggc tgtttcaaag ccagctccca
    26101 cttctgcagc tgaatggctg tcatcgccaa aacatactgg gatacctttt tcacggatca
    26161 tttttaaaaa atgaggtgca ggatacgggc aggcaaaacc ttttcgaagc ggcgaagtgt
    26221 ttatgtcaat aatgcaccca atttcacgaa tgctatgaag tgcgttttct gcggcacgaa
    26281 gaacaggcgc actttgaagc tctgatgtat ttcctgccat tttccgaatc aagtcaaaat
    26341 gtcccagaac ttcaggccgc agtgcttccg ccatattacg cacagtctga aaataggcta
    26401 tgcacaggtt ttccagactt ccgcagcgct gtaccgcctc gccaaataat gcccgagtat
    26461 agtctatgct gataccgttc acataatgaa ccgatccaac aatataatca aagccaagct
    26521 ctctttttag agaaagcatt ttgtccgcat agctgtcaca aggaataact tctgcttcaa
    26581 aaccttttaa caaagtaatc tttgagtccc agattctgct cagagaatct aattcgtgag
    26641 catagcgtcg gaatttattc agcagtattt ccggtgtcca gcccagagca agttcctcgc
    26701 tgtacatatg ttctgtttta aagcgaggcg catgttctgt gatgccatag atgcttatgc
    26761 ccgcatctac ggcagccgcc acaagtgcag gtaaagggtc ctgagcatga tcgcaatact
    26821 cgctgctatg gcccccatga agagagattt tccagtgcat ccttttcata acacggcaga
    26881 tcctttaaaa atcagttgaa aatttaatgt gaacttcccg gcgctgtttt caaaattcga
    26941 tgaaatacca tccgtttatt tgaggcattt tcgcatccgg cagaagattt tttttgtacg
    27001 attatgttta agttgcataa catagcataa gcgtcataaa atatagcaac ggaagaccgt
    27061 tttttagagt caagaaaagc accaaggaga aaaaaagtga aaatcgattt tggcggtgtc
    27121 gttgaagagg tcattacgcg aaaagaattt cccatgagca aggcaaagcg tgtgctcaaa
    27181 aatgaaacca ttgctgtcat cggttatggt gttcagggac ctgcacaaag cttaaacctg
    27241 cgcgacaacg gattcaatgt tattgtcggt caggcaaagg agtttaagcg ggactgggat
    27301 cgcgctgtaa aagacggctg gaaacccggc gtcagtcttt tttccattga agaagcttgc
    27361 gaaaaggcga ccgttattca gctgcttgta agtgacggtg cccagaagaa aatctggccg
    27421 accattaaga aactgctcaa gcccggagat gctctttatt tctcgcacgg gttcagcatt
    27481 acgtacaaaa aactcacaaa agtgattccg cctgattatg ttgacgtaat tctggttgcc
    27541 cctaaaggct ccggcacatc tgtgcgccgg aatttcctta gcggtgccgg tatcaattcc
    27601 agttttgcca tcggtcagga ctttacaggc aaagctgaag aacgggcgat ggcactgggt
    27661 atcggaatcg gttccggtta cctcttcccc accacttttg agaaagaagt tttcagcgat
    27721 ctcgtgggcg agcgtggtgt attgatgggc gctcttgccg gtatcatgga agcacagtac
    27781 gaagtcctgc gtaaaaacgg gcatagcccg agcgaagcct tcaatgaaac tgttgaagaa
    27841 cttacgcaga gccttattcg tcttgttgat gaaaacggca tggactggat gtacaataat
    27901 acttccgcaa cagcccagcg cggcgctctc gactggaagc ctaaatttaa aaaagcgacc
    27961 cttccgcttt tcaaagagct ctacaaacgg gttgccagcg gcgaagaatg caaacgcgtt
    28021 ctgtccagca caggcaaaaa gaactatcag gaaacactgc agaaagagct cgaagtgatt
    28081 cgcaattcgg aaatgtggcg tgcaggtgcc gcttcccgtg cgctcagacc caagaccccg
    28141 caaggtcagg ttgttaaatc caatgtgggt accgccggac ggtctcacaa ctgatctttt
    28201 taaggctgtg gcaggtaagc gggcaccgaa agctccttac ctgcccggcc tgcctcgaag
    28261 agtttttatt tgaattttgt gctttcttat tatggatgac gcgtgcagca ttagagctta
    28321 aattcgggct atcggcatta tccgggatta atgcataaaa gggatcaggt tttgcagcaa
    28381 gaaaagatag aggctttatt aggtccgata gacggacact attttaaagg ccagtattct
    28441 gatcccgaaa tttctattcg cttactgagt gatctcggga aaaagcggca gaataatgaa
    28501 gattcctgta tgctttatgt gcccggttcc gaggcactta ctgattcccg cggaatcctc
    28561 gtcgctgttg ccgatggaat gggcggagcg aaagccggag aatatgcgag ccataatgcc
    28621 ttgcgttgta tttgccccgt ttattatgaa agaaatccgc atgctctggt acctgcggcg
    28681 ctgaggcagg cggtggaggc agccaacgcc tttgtctttc gggagtccga gcataatatt
    28741 gatttttccg gcatgggcac taaagtgagt gctctggctc ttgtgggcaa ctgg
//
LOCUS       DAOPWB010000021        28485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1618_length_28485_cov_4.606753, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000021 DAOPWB010000000
VERSION     DAOPWB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 28485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28485
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1618_length_28485_cov_4.606753"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKN92_03480"
     CDS             complement(<1..322)
                     /locus_tag="PKN92_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ47886.1"
                     /translation="MKKNNRGFTLVELLVVMAIIAILASIVVPNVQRYIVRARVTRAV
                     AEISGMEMSLTAITTDAGRGNFGQMLKKNSVREVVPTDGPDSAPVQVANVQSAFPGDV
                     IQIGW"
     gene            complement(581..1822)
                     /locus_tag="PKN92_03485"
     CDS             complement(581..1822)
                     /locus_tag="PKN92_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ47887.1"
                     /translation="MPKYAYRALNRDGKQQFGIIQADTQALALNDVRALGLFPTHIKE
                     ATRADERRALRQGKGGIEELYFGGVKTKEIVVMTRQLSTLIDAGLPLLRCINVLIAQL
                     KPCKLRDILRETAADIQSGSTFAEALAKHPKQFDRLFVNMVRAGEVGGMLEVVLQRLA
                     LFMERRQALKRRVRGAMIYPIAVLLIAAGIVAFLLLKVVPVFADIFLEFGGDLPGPTK
                     ILIAAGDFMIYKWWVLVTVICWIIIVIKLMMRSKQVKRVFDRVILKVPLVGDLIVKVS
                     VARFSRTLGTLVTSGVPILQALKITRETIGNEVIQNACDAVYDSVQDGGTIAAPLDAS
                     KVFPAMVVNMIDVGEETGALDSMLMKVADIYDAEVEAAVEGMLALMEPLIIIVLGGII
                     GFIVVALYLPIFTLGDSITGG"
     gene            complement(1834..3582)
                     /locus_tag="PKN92_03490"
     CDS             complement(1834..3582)
                     /locus_tag="PKN92_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNZ47888.1"
                     /translation="MPAVLQKARPLGEILIDQGTISPLHLDEALQRQRLTGDLIGRIL
                     VSLGHATEQNIIEALGMQQGMVRVDLSKRNIPVEIIKMLSGDVAQFYGVIPISFENNT
                     LTVAMSDPLNMQTLDDLRQITGLDVQGAISTPEEVSAAWKKNYSFETGSVGAMLDELK
                     ELVGTEELSLEELGQQEILSNVDNLVELAQQPEVIKIVNLIFLTALQKRASDIHFEVY
                     EEDFRIRIRIDGVLHEVVHPPKASCIAIVSRIKIISNMDIGERRLPQDARIELKIGDS
                     LIDIRVATLPSLYGEGVVMRILDRTAVRIDLDQLGLSQGARKKVDTVINKPNGIMLVT
                     GPTGCGKTTTLYACLNILNTPEVKIITTEDPVELQIERLVQCQVHEEVGLTFAACLRA
                     ILRQDPDIVMVGEIRDLETAKIAVEASLTGHLVLSTLHTNSAPETITRLLDMDVEPFM
                     ITASLEAVLAQRLVRVLCKACRERYTPEKEEMDELSVPQAWRNDPKFAFFRPKGCAAC
                     DYVGYMGRTGLYEVMLVDEAVRELILERAMAHELRTYARKHQNMVILREEGLVKCIQG
                     ITSAAEVIAKTDRYED"
     gene            complement(3610..4731)
                     /locus_tag="PKN92_03495"
     CDS             complement(3610..4731)
                     /locus_tag="PKN92_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNZ47889.1"
                     /translation="MAYEIVSLLRMLVDRDGSDLHITEGVPPIGRVHGHLQFFGDNPC
                     TAEDTERLMKSIASVDNQQELQEVGGADFGFAFEDVARFRVSIFKQRGYVGLVLRLIP
                     RKILTFEEIGLPNGLKEVITQPRGLILVTGPTGSGKSTSLATMIDYININYDHHIVTI
                     EDPIEYYHTHKKSIIMQREVGVDVPTFSEALRRVLRQDPDVILVGEMRDLETIEAAVT
                     AAETGHLVFGTLHTTGAVRTIDRLVDAFPTNQQEQIRTQLSGNLKAVISQALIPRKSG
                     FGRVAAFEVMITTPAIQNLIRENKSYRITSAIQTGHKYGMNLLDEHLLALLRKGVCKY
                     EDLLAKAQMADEFETQARALGLLGDLPGGGAPGQARRAG"
     gene            complement(4738..6627)
                     /locus_tag="PKN92_03500"
     CDS             complement(4738..6627)
                     /locus_tag="PKN92_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNZ47890.1"
                     /translation="MQTMYSSFGKQLVDKKIITQQQLEEALHRQQTTMSNRKLGEILV
                     RLGYLSKSQIIEALAEQLGLPLIDLAERDIPPRIRALMEGSIATLYRVVPLEERGSTL
                     IVATSDPTNINTLDNLSRLLDRPIEPALATPEGITEALGRYYGHQKETVESMLSSASS
                     ASSVSSLSSFSNLGSGASGSMGSFASLSADDMSIDSIDLGDAQETMATVGDVHDDSPV
                     VRYVQQLIVEAYRLRASDIHIEPGKTTMTVRYRIDGVMQEMPTPPKRAQAAIISRLKI
                     MAGMDISERRIPQDGRIKMSVGAKDIDLRVSALPGVYGESVVMRILDKSGLLLGLGQL
                     GFCAEHQVQWERLIRHATGVVLVTGPTGSGKTTTLYGSLNTLNTPDTKIITVEDPVEY
                     QLTGINQVQINHEIGWDFARALRAIFRQDPDIVMVGEIRDQETAEIAIKAALTGHLVF
                     STLHTNDTASSFTRLIDIGIKPFLVASGVRAILAQRLVRTVCNGCREVYQPAEIEKDR
                     LEMDIDWSTVRLEHGAGCESCNETGYQGRLGAYELLLTNDALRVMLMRGESATALRRQ
                     ARIDGMITMREDAWNKAKRGLTTLEEVNRRTRVDEPLIRTEKADSETKAHPAMPAPAA
                     AGAER"
     gene            complement(7035..7838)
                     /gene="menH"
                     /locus_tag="PKN92_03505"
     CDS             complement(7035..7838)
                     /gene="menH"
                     /locus_tag="PKN92_03505"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HNZ47891.1"
                     /translation="MTEASSYPESKKTVLLLHGFMGSGEDWQFLPASFGAAYSFLCPD
                     LPGHGSAWNRNISPALNLDTVLLKLSTALDSHGAESCALVGYSLGGRIALHLALRYPR
                     RFSCLVLISASPGIEDPRARTQRQKADEQLARQLDTLTANSTAFQTFLLHWYRQPVFA
                     SLAEKPLLTRTLITRRMQNSPPALAQCLRSLGAGTLPSLWNRLSELNMPVLLIAGKDD
                     PKYVGISSRMHALLPAATLEILDHSGHCPHLEVPETCRDTVAAFLRRHC"
     gene            complement(7861..9705)
                     /locus_tag="PKN92_03510"
     CDS             complement(7861..9705)
                     /locus_tag="PKN92_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ47892.1"
                     /translation="MKKYSYTTVKLVLVLLLSAPIASALTVEEVLSAMPPDNAASASL
                     LFDKAFEEGPALIKALCARITPSADGVDAEAKFALYGLAKHAGRPGSTAQRTLMARIL
                     EEAVRQADSGEVKSFFLSILNFCADDASVAALAAWICDGEIALDVIRAIESIGGDAAL
                     TALSLADCPEMQEAIAQATARLQGQAPYTAEAAGLSDDVLRLVLNPDACENKTESAER
                     CRELLVDQTLSSSARCMVLRALVTLIGKEALPELIAAQTSPDRHYQGCARELVRFLPG
                     EDVSQAWTFRLSELDNAQRAAVISLLGERSDASARNAVFAALRDGQPDQRIAACEAVS
                     ASYGASAVSPLLDAFETAETDAELQAVKGALLRVPNLEEHVLALASADELASESFSDT
                     RKIICLDIIAERNARNFKNFVLACLEDADGKVRRSAFSALSVVGNEEDLTMLFDRLQH
                     EQRDAEAEAASASLVSLADRLGVKEQSIARAAELLGQSDRGTALRLIRTLAAFGTAEA
                     LVPVKDSISAALASGDVDAKWARNGLEVLAVWPLDDARNTLLDLWKGQESEDLRKTAL
                     KCYITSVQRTLTDKSDQIKALREAKEFTADDSEKRSLDDAASKIRGKK"
     gene            complement(9722..10993)
                     /locus_tag="PKN92_03515"
     CDS             complement(9722..10993)
                     /locus_tag="PKN92_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47893.1"
                     /translation="MQSDRLFSRRSFLKNTGKSVAAFTIVPRHVLGGAQFTAPSEEIT
                     RAIIGVGGMGSSHLGYAGARTVALCDVDRKHLEGALAKCEAGVDGYRHFREVLARPDI
                     DVVHIATPPHWHGIISAEAARAGKDIWCEKPMTRTIGEGRKVLEAVQRHGVLFRLNTW
                     FRFKDKFYDFDTPVQPIKKAVENRLFGWPLTVTVGPDTGFDFKFQWSGKTDLQPEPIP
                     EELDYDLWLGPAPWKPYNSHRVHSTFRGYWDYDGGGLGDMGQHYLDPVQYFLEKDNTS
                     PVAITVDAPLQHPEAASSWRRIEMTYADGCKIICTSEETGKDVPYISGPEGNLYKGMR
                     SDINGFAEKLASLPEPVWQESDFYRAVKNRRKFALNEENGHRSCTLVNLGVIAVRLGR
                     ELQYDPEKELFVNDDAANRLIHQPMRSPWTI"
     gene            complement(11189..11374)
                     /locus_tag="PKN92_03520"
     CDS             complement(11189..11374)
                     /locus_tag="PKN92_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNZ47894.1"
                     /translation="MWAPGLGELVIIFVIVLTLFGGGKLAGVGKSLGQAISEFKSAVN
                     EDKKEISAEKKEDTSGE"
     gene            11700..13067
                     /locus_tag="PKN92_03525"
     CDS             11700..13067
                     /locus_tag="PKN92_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47895.1"
                     /translation="MSPYLLTRRAFIASATTSVLATGAFAARPNTARVVPGKLSPNEK
                     LNVAGIGAGGKGTQDILSFQRENIVALCDVDAGRAAEAFYRVPEARQFQDYRKMFDEM
                     GSEIDMVTISTPDHTHAPAAYRAMKLGKHVYVQKPLTHTVAEARLLTRTAREMGVATQ
                     MGNQGHSGDGVRDLAEMLWSGVIGTVSEVHVWTDRPGSRWPQGEPTALPPAEPVPEGL
                     NWDLWLGAAPWREFNGLYAPFKWRGWWDFGCGGLGDMACHVMDPAFFALKLGEAPSFT
                     VEPVRMESANGQTYPKNIILKYAFPARADMPPVDLYWYENGLMPPRPEGIPEGDVLGD
                     KQNGSLFIGSKGFLTAGEYGGNARLLPDSIMADYKKPDQTLPRVSGANHYRNFLDACK
                     GNAPAVSNFDYAGPFTEMVLLGNVALRLNKAISFSTESMQITNDAAAQTLLTKPYRTG
                     WELPV"
     gene            13116..14096
                     /locus_tag="PKN92_03530"
     CDS             13116..14096
                     /locus_tag="PKN92_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47896.1"
                     /translation="MKTIFHLTLLLLFAVSVSVAAEEAPLKLGLIGLDTSHVIAFTQV
                     LNDASRENHVPGGRVIAAYKGGSADLESSASRVDGYTEQLVKEYGVQIVSSIEELCTL
                     VDAVLLTSVDGRPHLAQATPVIKAGKRLFIDKPLAGSLADARAIAALAKEHNVPWFSS
                     SSYRFYKSLQELKQQDVGEVRGAISYGPASLDPTHPDLFWYGIHATEALFTIMGTGCQ
                     RVTCFVSDDFHVATGEWEGGRMGTLYGLRTGAAPHRVTVFGSKAVADQQGSGDYAPLV
                     AEIIQFFRTGVPPVSPDETLEMFAFMEAAHESIRQGGAPVALDEVIRQSE"
     gene            14280..15338
                     /locus_tag="PKN92_03535"
     CDS             14280..15338
                     /locus_tag="PKN92_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNZ47897.1"
                     /translation="MKRKGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQNN
                     LKQWGIVFKMYSNEAPGEKYPPLQTYRDRTKFASATCNPGANAVKDALAGGPSVYAIF
                     PEYLTDTNIIWCPSDPELGQHKASMFAKPGELPGCIEDGGTKLGWSPDAIDDSYFYLG
                     WAFDRLETSERTSQFGVVGALLSGTFTGDPWVPVQIGAALDGLASKYPAEIAAMLGGG
                     DVYGAAAAVDKDIELVAPWKGMHAGNGESDTVYRLREGIERFLITDINNPAASAMAQS
                     TLFIMLDTMGAGANTKYFNHVPGGCNVLFMDGHVEFLRYTGVSVDNLTNSAEVSQKMQ
                     GCQSPVLPTLATLVAAFN"
     gene            15555..15758
                     /locus_tag="PKN92_03540"
                     /pseudo
     CDS             15555..15758
                     /locus_tag="PKN92_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            16140..16577
                     /locus_tag="PKN92_03545"
     CDS             16140..16577
                     /locus_tag="PKN92_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47898.1"
                     /translation="MSGQNDAAVIDAAKVFDKDLRVSSDYMGAGVGNGHGDTVYRLRE
                     GIERFLITDINNPSASAKAQSSLFIMLDTMGAGAGTRFFNHIPGGCNVLFMDGHVQFK
                     RYIPMNIDAIATAAEMAAKMQACEEPVLPTLATMVDAFRSAQD"
     gene            complement(16703..20968)
                     /locus_tag="PKN92_03550"
     CDS             complement(16703..20968)
                     /locus_tag="PKN92_03550"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47899.1"
                     /translation="MKMSARHMMKWLVCVVAAVLLSGCPFSGELLVQPGSVSLSINQS
                     TAQISVINAGSGTLSWTARAGQPWLLLRLGEEGSAEAQISGKTVKTDIITLFLDASRL
                     PGTDERLRGTVFNTSGGGDEEISVTFEPDRQPRLTIQPVTLHFKSSETKKTIDLTNEG
                     AATLLWQSRVASASPWLSVTPSSGETTAGSAQVLSIAVDRAMLTPAAEPYQGELVFES
                     NGGRQSLAVTVDVGAFSLSPLELDFGLIAAPKTLLASLRTQSPEAVVLTSEIEYGSGN
                     AWLSVTPAALQASRTKPLDLSITANPAGLAPGNYSAVLKLSHVPTGQQEQISLKMEVG
                     KAADFLIDPDEINFGDLNGTATATLTLTNAGDAPFTYQVQKQSPAPWLTISPESAAIT
                     NTGTVTLVADTTRQPAGSAQVTLEFKAGAVLKLVRVSVNRTPDPKKDKLEVEPRDLNF
                     GSRENKKDINLWNEGPGAINWSIDTSDFPAWLSISSTGGSLSGAFTETVSFAINRELA
                     PAGEENFSHTVKVQSSLPDAEPVEVTVRAQARRFPQISLTGEGVDVTNIPFIMMDINE
                     NSAQFSVENLGNAALTWNVADDHLPDWISSITPLQGDLAPGKMQKVTVTTDRTGLDNR
                     GGTYRLPIRSNDTEAPTSFLDVHVRVPYSIVIGVQPPKLNFGRSQSTLTFQVANLGDA
                     GVPLDYIVTSSQPGWLFAEPARGRSMGTAGAVKDWQFVSVAIDRSSITGTGAAATLLI
                     TADNVPPNASPVVPVEVTVQLDLAELSIESASPQFRPPSLIRASVLLRDIRQRTFPMF
                     KDDLLDSRTLYRLATPRVDILENSLPVDLAETNVFVKKDESLSFAMLILLDFSGSMAQ
                     AARALVDDGQLQPAPGEDPLTALYREAVGGILDEVPPHYRVGLAVFNERNPWWGGAMR
                     ILTGAPAGYDSKASGASFTSNQDILRYRLNTLQVVDNGATPLYTAIAEGALALFRLGG
                     NMPDFDNLGQCILLPITDGKRTTPPGEITALTEILRATRTRTFTIGWGSEVYANSLIQ
                     IADESGGHYYATSTKRIPGTVGADGNPLTLPVKAELMKWCKKDPDDENAPTIVSDLKS
                     HIVLSYVTLNEESSMDIQARFELDVATPPLKESLYVSQIPSIEFANDVRLGQIGTHTD
                     GIRSDGSASVRFYADYIPRNITRLVFELNASDGSPWSASLLPAEQGGLVAHWSMSRNG
                     NMLILETPSAGRALDYGDYGNLFQLDFTGLSAPVYLSVNMLEPVIGGGADGKYFTIPR
                     GIDIGYEEMKAVSFPNPAFAFLPPLLSPESSIIDLGNLKDLPEDERQGLVQVRNIGGE
                     HMPTFAGLHWSVREGTEWLPGTFPGKLSFVYDTTLPPVYSSFYSIYETAEAAVVPIGH
                     YDTGGVLISDPGPYSRAFYIDVTYGSLFYQFSYGPYYITYEVE"
     gene            complement(21009..24854)
                     /locus_tag="PKN92_03555"
     CDS             complement(21009..24854)
                     /locus_tag="PKN92_03555"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47900.1"
                     /translation="MKKRLDLAVSLAAFVCLVLVIQGCTATPKFSVEPGSISFGSTVF
                     ERRVTLKNEGTRALNWSVDTVMRAHADAPWTTASVSWLACEKDAGSLKPGVENLVVTA
                     NRSALEPGLYDNVGLRFICGSKQQIVPVSLTVAPLLKATPSKVYLKPDARNASFELEN
                     QGDAAVTWQVRYFEGIAGQEESLPGDMMLTPAPGRLSGKSRATVRLEWTRERGDFGLK
                     IFNVDAPGHFAVVYFTFSKPLGTLGVDPETLRVYYQSSPAFQGGSGAEQPVSRLTLKN
                     KGNTSLSWTLSVNGIGGVAADIFQITPVLGTLQPGQEGEVLVSVSNAAAAIPGSGNYE
                     LRIDVPGADGTVIVPLVLEVVSLPVVVASDPPEPHAIRPTPTYMTLLDFGREETSKKF
                     FVFNAGALDSRLYFRITHDDENAESPLVVDVSPMRGNTTGPNQVFYLASVDRMVDAQQ
                     IVVTVDRSAMKEDLEYRNLTIEAWDEEGTGPIAAVEPWTVQIKVERAPMTIEGALNRS
                     RPPYLMRFALLLRDMLGEVIPTRTPEDLGKLSFEIQEDGEVLDLNETAMQVNGPENLK
                     VNLVLLLDYTGSMYKAGVGDPESPRLPGEVLEEIKESASMFLDDLPPGYRVALMYHND
                     RQPRSRMIHGFSTDRASLKAALKRFSVPPALHGTSDVYDALIEAIQRLAAEDAEDVLP
                     FDDADLRSVVFISDGKDNSSTQDAAAVVTAARNQKVRLYPLTYSAGSAVNYPDMIKLA
                     DETGGHLYRAGNAANLVTFLGHRRSLVLKPVQDTGAVSSTAAFIVKNAGKDPLSWQVK
                     RQNHADWLRTVSPADGTLAPGEEVQVLLSLDPALLNTPGKAGLALLDILSSDGSGAAE
                     LFLALNDAGTDVQQLSLSLRDEPGRIWNELSNQIVLSYVTPLQRSGKYSIIATWRTPQ
                     GKDVRAMFEEDGMYYHGDVHGGQLSLYTTGISLNRTAASDAERARAEVYVRADYVPRN
                     VNFFRMRFISQLDAQAPAAVRAAFDAAALTVEIDQEGLLYFSDGSKPNWRMISKGNHV
                     YELLTTEENPLPYASSGNLLRIRFDNLLPFVDACAVAGIPAEFYLGMRVDNSVYYAPA
                     SNTEPSGTVYFTYPSGPLNPDRSLWIGDASDLAAPARSIADLAAPGINPEAPDAWDRD
                     GDGLPDFDDPYPDDETRPGRILQPESLSFNAAGDTARAVTITNNRWDTFNITALSVEI
                     IGGTPLQPDQFSWSLEGDPAVADLIDSDEPIRVQPGETLHCTLFFDPASLAAGNYSAR
                     IRLKNDLYPDELTLINIGL"
     gene            complement(24937..25524)
                     /locus_tag="PKN92_03560"
     CDS             complement(24937..25524)
                     /locus_tag="PKN92_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47901.1"
                     /translation="MGFHQRQVLVYTGIAAVLLIGLSACSPVVFVPDKALEAAIRQEL
                     SKPLSLFLTRADLEKITTLNAAALNIERLEGLQYCKNLRKINLNDNAVRNIDPLSSLT
                     KLTHVHLRGNRIVEIEALAGLLHLEVLDLSGVDNVIADWKALQDNVLNGGLGAGNVVL
                     LSALNTLDKDDHPLPGFAPVYDTLLQEGVVVEFLD"
     gene            complement(25681..25857)
                     /locus_tag="PKN92_03565"
     CDS             complement(25681..25857)
                     /locus_tag="PKN92_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47902.1"
                     /translation="MKHLAPVTRRSVKKAGSWEDFVCVVATLFNNIIGAFGGSAPISG
                     YIADKCTIKPPENR"
     gene            complement(26121..27335)
                     /locus_tag="PKN92_03570"
     CDS             complement(26121..27335)
                     /locus_tag="PKN92_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ47903.1"
                     /translation="MPRYEYEVKKGPGEVVNGVLEAENKRAAVARLREMGYFPIRVDE
                     QVEEESKDTLREAMKRIRVKDRNIFLRQLANLSESGMMITRALRTLVDQTENPKLARI
                     VDRIRDDVQKGASLADAMEKHPQVFPSMYCSLIRAGETGGMLEEVLWRICAFGEQEEE
                     LRGKAVSAMIYPTFLLIVGSAAIFILISFVFPKFITIFEDFNAQLPLPTVIVMKICDF
                     MGTWWWAVLLGIVGTVVLLIRYYRTEPGKRKFDGWALKIPAVNTLIIKYEMAKFSRTL
                     GTLLDNGVPVLTSLKITADTMSNLLVRDRVAELHNGVTEGESLSETMREGPLFPPMVV
                     SMFAVGEESGRIGDVARRIADAYDLEVDRAVKALTALFEPLLIVIMGVVIGFLVIAML
                     LPMLTLSSNIGA"
     gene            complement(27339..>28485)
                     /locus_tag="PKN92_03575"
     CDS             complement(27339..>28485)
                     /locus_tag="PKN92_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNZ47904.1"
                     /translation="SDTTDIHIEPFEDSLRVRYRIDGILHQAPTPPSIRGFHAAIVSR
                     VKIMANLNIAEKRLPQDGKILANFGENRYDLRVSILPTPHGETVNIRILSRSSMALSM
                     DQLGFLPEDMEQFNRFISRPHGIILVTGPTGSGKTTTLYAALAKLNQVDRKILTIEDP
                     IEYQLSGISQMQVQPRIGFDFSVGLRSMLRHDPDIMLVGEIRDYETAEMAIRSSLTGH
                     LVLSTLHTNDSAGAVTRLIDMGIEPFLIASTLIASVAQRLVRKNCSYCSEAFEPDREL
                     LKAEFDLSDKDLEGARFMRGKGCEQCRYTGYKGRLAIYEMLPFTEAIKDMTLQRVNAG
                     IIKKEAMKRGMRTLRDAGWRRVYSGDTTAEEVLRLTADTDPIHQGDA"
BASE COUNT         6599 a         7904 c         7508 g         6474 t
ORIGIN      
        1 gccagccgat ctggatcacg tctcccggaa aagcgctttg tacattggca acctgcacgg
       61 gggcgctgtc cggtccgtct gtaggcacca cttcacgcac cgaatttttc ttgagcatct
      121 gcccgaaatt gccacgaccc gcgtcagtcg taatcgctgt cagtgacatc tccatgccac
      181 tgatttccgc aacagcccgt gtgacgcggg ctcggacgat ataacgctgc acattgggca
      241 ccacgataga ggcgagaata gcaatgatcg ccatgaccac cagcaattcc accagtgtaa
      301 aaccccggtt atttttcttc atacctgacc tcctttaggg cgtgcatcag cacgcatcta
      361 cctgcaaaaa atgattaaaa atataccttg cataccgaaa ggcacagtgc atttcatgcc
      421 tttttcaaag gcaatacaag cgcccagcca gtaagtatac tatagtttac cctattctta
      481 taaaaaagtg tgactttttt tgtatttttt aacaaaattc cagcgaaaac tccagcgcac
      541 tcaaagcaga gcttctgcag gattctgtgt tcaaatccga tcagccgccg gtaatgctgt
      601 cgcccagcgt gaaaataggc aggtacagcg cgacgacaat gaaaccgata ataccgccca
      661 gcacaataat aatgagcggt tccatgagag caagcatgcc ttccaccgcc gcctccacct
      721 cagcatcata aatatccgcc actttcatga gcatggagtc cagcgcaccg gtttcctcac
      781 ccacgtcgat catattgacc accatggcag gaaacacttt actggcatcc agtggtgcgg
      841 caatcgttcc gccatcctgc acgctatcgt atacagcatc gcaggcattt tgaatcactt
      901 cgttgccgat ggtttcccgg gtgattttca gtgcctgaag aatcggcacg ccggaggtga
      961 ccagtgtgcc gagggttcgg gagaaacgcg ccaccgacac cttcacaatc agatcgccca
     1021 caagcggaac tttcaggatc acccggtcga aaacgcgttt cacctgcttg gatcgcatca
     1081 tcagtttaat gacgatgata atccagcaga ttacggtaac cagcacccac catttataga
     1141 tcatgaagtc cccggcggca atcagaattt ttgtgggacc gggaagatcg ccaccgaatt
     1201 ccagaaagat atccgcgaaa acgggcacca ctttaagaag caggaaggcg acgatgccgg
     1261 cagcaatgag cagcaccgca atgggataaa tcatggcgcc ccgcacacgc cgtttcagtg
     1321 cctgacgccg ttccatgaac agggcaagcc gttgcagcac gacttcaagc ataccgccga
     1381 cttcaccggc gcgtaccata ttgacaaaaa ggcggtcgaa ctgtttggga tgcttggcaa
     1441 gggcttcggc aaaagtggaa ccgctctgaa tatcagccgc cgtctcccgc aggatatcac
     1501 gcagcttgca cggcttcaat tgggcaataa gcacattaat gcagcgaagc aaaggcagac
     1561 ccgcatcaat cagcgtggac agctgcctgg tcatcaccac aatttccttg gttttgacgc
     1621 cgccgaaata gagctcttca atgccgccct tgccctgacg cagcgcacga cgttcatcgg
     1681 cgcgggtcgc ctctttaatg tgggtcggga aaagcccgag cgctcgcaca tcgttcagcg
     1741 ccagcgcttg cgtatcggcc tgaataatac cgaactgctg cttgccgtct cgattcagtg
     1801 ctctgtaagc atattttggc atagctgctt ctcctagtct tcataccggt cggtttttgc
     1861 aatgacttcc gccgcgctgg taatgccctg aatgcatttc accagccctt cttcccgcaa
     1921 aataaccata ttctgatgtt tacgggcata tgtgcgcaat tcatgcgcca tcgcacgttc
     1981 aagaatcaat tcgcgaaccg cctcatccac aagcatcact tcataaagtc ccgtacggcc
     2041 catgtatccc acataatcac aggcagcgca gcctttcggc cggaaaaaag cgaatttcgg
     2101 atcgttgcgc caggcctgag ggacactcaa ctcatccatt tcctcttttt caggcgtgta
     2161 ccgttcccgg catgccttgc acagcacacg caccagacgc tgagcaagca ccgcttccag
     2221 tgacgctgta atcatgaaag gctccacatc catgtcgagc agtcgggtga tcgtttcagg
     2281 tgcgctgttc gtatgaagcg tactcaacac caggtgaccg gtaagggatg cctccacggc
     2341 aatttttgcc gtctcaaggt cacggatttc accgaccatc acaatatccg ggtcctgtcg
     2401 aagaatggcg cgcaggcagg cggcaaaggt aagccccacc tcttcatgca cctgacattg
     2461 gacaagccgt tctatctgaa gctccaccgg atcttctgta gtaattatct ttacttccgg
     2521 tgtattcaat atattcagac aggcatagag cgtggtggtc ttgccgcagc ctgtcggtcc
     2581 ggtgaccagc atgatgccgt tgggcttatt gattacggta tccacttttt ttcgcgcacc
     2641 ctgtgaaagg cccagctgat caaggtcaat acgaacagcc gtccggtcaa gaatacgcat
     2701 cacaacacct tctccgtaga gagagggcag cgttgccacg cggatatcga taagactgtc
     2761 accgatttta agttcgatac gggcgtcctg cggcaggcgc ctttcgccaa tatccatatt
     2821 ggatatgatt ttgatacggg aaacaatggc aatacaggaa gctttcggcg gatgaaccac
     2881 ttcatgaagc acgccgtcaa tacgaatacg aatccggaaa tcttcttcgt aaacctcgaa
     2941 atgtatatcc gaagcacgct tctgcagcgc tgtcaaaaaa ataaggttga ctatcttgat
     3001 cacttccggc tgttgtgcca gttcaaccag attatcaaca tttgacagaa tttcctgctg
     3061 gcccaactct tccagggaga gctcttctgt gccgaccagt tccttcagct cgtccagcat
     3121 ggcaccgaca gaaccggtct cgaaggaata attctttttc catgcagcac tcacttcttc
     3181 gggtgtgctg atggcaccct gaacatccag tccggtgatc tgccgaagat cgtccagggt
     3241 ctgcatgttg aggggatccg acatggcaac cgtcagtgta ttgttctcaa agctgatcgg
     3301 tatcacaccg taaaactgcg ccacatcgcc cgacagcatt ttgatgattt ccacggggat
     3361 attgcgcttg ctgagatcca cgcgaaccat gccctgctgc atgcccagcg cttcaataat
     3421 attctgctct gtggcatgac caagggaaac gagaatacgc ccgatcaaat ccccggtcag
     3481 acgctgacgt tgaagcgcct cgtcaagatg cagaggagag atcgttccct gatcaatgag
     3541 gatttcaccg agagggcgtg ctttttgtag aacggctggc ataaataaaa acgtgtttcc
     3601 ttaatacgct caaccggcgc gtctggcctg gcccggagca ccgccgcccg gaagatcacc
     3661 gagcagaccc agcgcacgcg cctgcgtttc aaattcgtct gccatctgtg ccttggcaag
     3721 aaggtcttcg tatttacaga cccctttgcg aagcagcgcc agaaggtgct cgtcaagaag
     3781 attcatgccg tatttgtgcc cggtctgaat ggccgaggta atacggtagg acttgttttc
     3841 acggataaga ttctgaatag caggcgtagt aatcatcact tcaaaggccg ccacgcgacc
     3901 gaatccgctc ttgcggggaa tcagcgcctg agaaatcacc gccttgaggt tacccgaaag
     3961 ctgcgtacgg atctgctcct gctggttcgt cgggaaagcg tccaccagac ggtcaatcgt
     4021 acgtaccgca cccgttgtat gcagcgtgcc aaaaaccagg tgacccgttt cggctgcggt
     4081 taccgccgct tcaatcgttt ccaggtcgcg catttcaccc accagaatca cgtccgggtc
     4141 ctgacgaagc acacgccgga gcgcctcgga gaaggtgggt acgtccacgc ccacttcgcg
     4201 ctgcatgata atactctttt tgtgggtgtg atagtattcg atggggtctt caatcgtcac
     4261 aatatggtgg tcataattga tgttaatata gtcaatcatg gtggcaagcg aggtggattt
     4321 tccggaacct gtcggccctg tcaccaatat aagaccacgg ggttgcgtta tcacctcttt
     4381 caaaccattg ggaagaccga tctcctcaaa agtcaggatt ttgcgcggaa tcagacgaag
     4441 caccagcccc acataaccgc gctgcttaaa gatggaaaca cgaaagcgcg ccacatcttc
     4501 aaaagcaaaa ccgaagtcgg caccgcccac ctcctgcagc tcctgctggt tatccacaga
     4561 agcgatgctt ttcatgagcc gttccgtatc ttcagccgta catgggttgt ccccgaaaaa
     4621 ctgaagatgc ccgtgcacgc gaccaattgg ggggaccccc tccgtaatgt gcaagtcgga
     4681 gccgtcacgg tcaacaagca ttcgcagcaa acttacaatt tcataagcca taaaaaatta
     4741 cctttcagca ccggcagcag ctggtgccgg catcgcaggg tgtgcttttg tttcggaatc
     4801 cgctttttcc gtacgaataa gcggttcatc cacacgggta cgccggttga cttcttccag
     4861 tgttgtcaac ccccgtttgg ctttgttcca ggcatcctca cgcatggtga tcatgccgtc
     4921 aatccgggcc tggcggcgca atgccgtagc gctttcaccg cgcatcagca taacacgcag
     4981 ggcatcgttg gtcagaagca actcataagc gcccagccgt ccctggtacc ctgtttcatt
     5041 acagctttca cagcctgccc cgtgctcaag ccgcactgta ctccagtcaa tatccatttc
     5101 cagacggtct ttctcaatct cggcaggctg atagacctca cggcagccgt tacacaccgt
     5161 gcgcaccagc cgctgtgcaa gaatagcgcg tacaccggaa gccacaagaa aaggcttgat
     5221 gccgatatcg atcaaacggg taaaagaact ggccgtgtcg ttggtatgca gtgtagagaa
     5281 aacaagatgc ccggttagtg cggccttaat ggctatttcc gccgtttcct ggtcacgaat
     5341 ttcaccgacc atcacgatat cggggtcctg acggaaaata gcacgaagcg cccgggcaaa
     5401 gtcccagccg atttcatggt tgatctgaac ctgattaatg ccggtcagct gatattcgac
     5461 cggatcttcc actgtaataa ttttggtatc cggcgtgttc agcgtattga gcgagccata
     5521 cagcgtggtt gttttgcccg aacccgtagg tccggtcacc agcaccacac cggtagcatg
     5581 acggatcaga cgctcccact gcacctgatg ctcggcacaa aagcccagct gacccagacc
     5641 cagcagcaga ccgctcttat caagaatacg catcaccacg ctctcgccgt aaacgccagg
     5701 aagcgcactg acacgcaagt caatatcttt ggcaccgaca ctcattttga tgcgcccgtc
     5761 ctgagggata cgacgctcgg aaatatccat acccgccata attttaaggc gggaaattat
     5821 ggcggcctga gcacgtttcg gcggtgtggg catttcctgc atgacaccgt caatacgata
     5881 acggaccgtc atggtcgttt taccgggctc aatatgaata tcactggcac gcaggcggta
     5941 cgcctccacg atcagctgct gcacataacg cacgacaggg ctgtcgtcat ggacatcgcc
     6001 aaccgttgcc atcgtttcct gagcatcgcc cagatctatg ctgtcaatgc tcatatcatc
     6061 agcggacaga gaggcaaaag agcccatgga gccactggca ccggagccga gattggagaa
     6121 actgctcaga gaactcacac tggaggcgct gctcgccgaa gaaagcatgg actcaacggt
     6181 ctctttctgg tgaccgtagt aacggcctaa tgcctctgtg atgccttcgg gcgtggcgag
     6241 tgccggttca atcgggcggt caagcaggcg ggacaggtta tccagcgtgt taatattggt
     6301 agggtcggag gtggctacaa tgagcgtgct gccacgctcc tccaaaggca ccacacggta
     6361 cagcgtggca atggagcctt ccatcagtgc ccggatacgg ggcggaatat cacgctccgc
     6421 cagatcaatc agcggaagtc ccagctgttc ggcaagtgct tcgataatct gcgatttgct
     6481 gagatagccc aaccgcacca gaatctcgcc caatttgcgg ttgctcatgg tcgtttgctg
     6541 gcggtgcagg gcttcttcca gctgctgctg ggtgataatt tttttatcaa ccagctgttt
     6601 tccgaaacta gaatacatcg tctgcatgcg agattacctt tgcgtcactc gcctcaactg
     6661 taccaaaaaa gcactttcgt gtcaatattt tcacagtaaa gacaacacaa actgccgaga
     6721 aaacaaggct attccacctg gtctccggca gagaaagcat ttcttctgtt ctgaaaaatt
     6781 cgctacttta tgtcaataat taacctgatc atactggctc cggcatccga caatacggac
     6841 acctctatac tattgtctct cataaatagg tcggctgtca actctaaaag tgtttatagc
     6901 cgtaacttat tcaaaatcca ccttctatgg cgcctcaaaa attgatgctc ttttaccttc
     6961 tggcgacgta aaaaaacgcc ttactccggg agggcagcgc agccgtgtgt ctgaaaaaca
     7021 ggcttctgtg actgtcagca gtgcctgcga agaaaggcgg caacggtgtc tctgcaggtt
     7081 tcggggactt ccaggtgggg gcaatgaccg ctatgatcga gaatctccag agtggcggcg
     7141 ggcaggaggg catgcatacg gctactgatt ccgacgtatt tcggatcgtc ttttccggca
     7201 atcagcaaca cgggcatgtt taattcggag agacggttcc agagggaagg gagcgtgcct
     7261 gcaccgaggc tgcgcaggca ctgtgccaat gcaggcgggc tgttctgcat gcgccttgtg
     7321 atcagtgtgc gggtgagcag gggcttttcc gccagcgatg caaagaccgg ctgccggtac
     7381 caatggagta aaaaagtctg aaaagcggta ctgtttgccg tcagggtatc caactgtcgg
     7441 gcgagctgct catcggcttt ctgtctttgt gtgcgtgcgc gcggatcttc aatgcccggc
     7501 gaggcggaaa tgagaacaag acaggaaaat cggcggggat aacggagcgc cagatgaagt
     7561 gcaattctgc cgcccagaga ataaccgaca agggcgcagg actccgcacc gtggctgtcg
     7621 agggctgtac tcaacttcag gagcaccgta tccagattca gcgcaggtga tatatttcta
     7681 ttccaggcgc tgccatgccc aggcagatca ggacataaaa aagagtaagc ggcaccgaat
     7741 gacgccggca aaaactgcca gtcttctccg cttcccatga acccgtgcaa caggaggact
     7801 gtttttttcg actcgggata agaggaagct tcagtcattc ggtgccgctt ttattccgta
     7861 ttatttttta cctctgattt tggaggcggc atcgtcgagg ctgcgttttt cgctgtcatc
     7921 cgcagtgaat tcctttgcct cacgcagtgc ctttatctga tcagatttgt cggtaagggt
     7981 acgctgcacg gaagtgatat agcatttcag tgctgttttg cggaggtctt ccgattcctg
     8041 gcctttccag agatccagga gcgtgttccg cgcatcgtcg agaggccata cagccagcac
     8101 ttccagcccg ttccgtgccc atttggcatc aacatcaccg gaagcgaggg cggcgctgat
     8161 gctgtccttc acgggaacca gcgcttcggc agtaccgaag gcggcaagtg ttcggataag
     8221 acgcagcgcc gtgcctctgt ctgactgtcc gagcagttca gcggcacggg cgatactctg
     8281 ttctttgacg cccagacgat cggcaagcga aacgagagac gcggaagcgg cttcggcctc
     8341 ggcatcccgc tgttcgtgct ggagccggtc gaaaagcatg gtcaggtctt cttcattgcc
     8401 gactacggac agagcggaaa atgcactgcg ccgtactttt ccgtcagcat cttcaagaca
     8461 ggcgagcaca aagtttttaa agtttcgggc attacgctct gcgatgatgt caagacagat
     8521 gatctttcgt gtatcggaga aactttcgga agcgagctca tcggcggatg ccagcgccag
     8581 cacatgttct tcaagattcg gcacacgcaa aagtgcgccc ttcaccgcct gcagctcggc
     8641 atcggtttcc gctgtctcaa aagcatcgag cagtggcgat acggcactgg ccccatagga
     8701 agcgctgact gcttcacagg cggcaatacg ctgatcgggc tgtccatcgc gaagcgctgc
     8761 aaatacagcg ttgcgtgcag acgcatcgct gcgctctccc agaaggctga tcacggcggc
     8821 acgctgcgca ttgtccaatt cgcttagtct gaatgtccac gcctgcgaca catcttcgcc
     8881 gggaagaaag cgcaccaatt cgcgggcaca gccctgatag tgtctgtccg gtgaggtctg
     8941 ggctgcgatc agttcgggca atgcctcttt gccgatgagc gtcacgagtg cacgcagcac
     9001 catgcatcgt gcagaagaag aaagagtctg gtcgacaaga agttctcggc agcgctcggc
     9061 gctttccgtc ttgttttcgc atgcatcagg attcaggact agtcggagca cgtcatccga
     9121 aagccctgcc gcttcagcgg tataaggcgc ctgtccctgc agtctggcgg tcgcctgtgc
     9181 gatggcttcc tgcatttcgg ggcagtcggc gaggctgagg gcagtgaggg cggcatcgcc
     9241 tccgatactt tcaatggcac ggatgacatc gagggcgatt tccccgtcgc agatccaggc
     9301 tgccagtgcg gcgacggatg catcgtcagc acagaagttc aggatcgaca ggaaaaaaga
     9361 ttttacctcg cctgaatctg cctgccggac ggcttcttcc agaatacgcg ccataagggt
     9421 gcgctgtgcg gtactgccgg gtcgccctgc gtgttttgcc agcccgtaga gggcaaattt
     9481 ggcttcggcg tccaccccat cggcagaagg ggtaatccgg gcacaaagtg ctttgataag
     9541 tgcaggccct tcttcaaaag ccttgtcaaa aagcaggctt gcggaggcgg cattgtcggg
     9601 cggcatggcg gacaatactt cctccacggt caatgccgat gctatcggtg cgctgagcag
     9661 cagaaccaga accagtttta cagtggtgta tgaatacttt ttcatgatct tgttccttcc
     9721 cttagatggt ccacgggctg cgcatgggct gatggatgag tcggtttgcc gcatcatcgt
     9781 tcacgaagag ctctttttcg gggtcatact gcaattctct gccgaggcgc acggcgatga
     9841 caccgaggtt gacgagtgta caggaccggt gtccgttttc ttcgttgagc gcaaatttcc
     9901 gccggttttt caccgcccgg taaaagtcgg attcctgcca tacaggctcg ggcagcgatg
     9961 cgagtttttc ggcaaagccg ttgatgtcgg agcgcatgcc tttgtagaga ttgccttccg
    10021 gcccggagat atagggcacg tctttgccgg tttcctcgga ggtacagatg atcttgcagc
    10081 catcagcata ggtcatttcg atgcggcgcc agctgcttgc cgcttcggga tgctgcagcg
    10141 gagcatcaac cgtaatggct acggggcttg tgttgtcctt ttcgaggaaa tactgtaccg
    10201 gatccagata atgctgaccc atgtcgccga gaccgccgcc gtcatagtcc cagtagccgc
    10261 ggaaagtact gtgaacgcga tgcgaattgt aaggtttcca gggcgcagga ccgagccaga
    10321 gatcataatc cagctcttcc gggatcggtt cgggctgaag atcggtcttg ccgctccact
    10381 gaaatttgaa gtcgaagccg gtatccggac cgaccgtgac agtcagaggc cagccgaaaa
    10441 gccggttttc cacagctttt ttgataggct gaacgggtgt atcaaagtcg tagaatttat
    10501 ctttaaaacg gaaccaggtg ttgagccgga aaaggacacc gtggcgctgc acggcttcca
    10561 gtactttcct gccctcgccg atggtgcggg tcatgggctt ttcacaccag atatccttgc
    10621 ctgcccgtgc cgcttcggca ctgatgatgc cgtgccagtg cggcggcgtt gcgatatgca
    10681 ccacatcgat gtccggacgg gcgagcactt ctctgaaatg gcggtatccg tccacacctg
    10741 cctcgcattt ggcaagtgcc ccttccagat gtttcctgtc cacatcgcaa agagcgacgg
    10801 tgcgcgcgcc ggcatagccc agatgactgc tgcccatacc gcctacgccg atgatggcgc
    10861 gggtgatttc ttcgctcggt gccgtgaact gggcaccacc gaggacgtgg cggggtacga
    10921 tcgtgaaggc ggcaacagat tttcctgtat ttttcagaaa gctgcgccgt gaaaaaaggc
    10981 ggtcggattg catatcagac tctcctgtgt tgatgtcttt tcctcaatgc ggtccgtgcg
    11041 gacctggaac cgaaaaaaaa caggcgcact ccgattagcg gttcaaaccg gaactgcgcc
    11101 tgaaaaggat tcaaaataat ggagacaacg cttgctgaag gcagcgagtc gaggaggttt
    11161 ttttgaatta aaaacacacc tgcgcgcctt actcgccgga cgtgtcttct tttttctcgg
    11221 cagaaatctc ttttttatct tcgtttaccg ctgatttgaa ttcagaaatt gcctgaccga
    11281 gcgatttgcc tacgcccgca agtttgccgc cgccgaataa ggtcagcact ataacaaaaa
    11341 taattaccag ctctcccagt ccaggggccc acatagtttt atctcctgtt gtattggttt
    11401 tcagacacac ctcttgtgtg tcggtttcag tatcaacgtt tccggcaaga caaaagagaa
    11461 cactgcgaaa cggtgaagat tctaaagtat agtctttttt tttgccagta gcaaataaat
    11521 agcggcgccg tctctgtccc ggtgtaaaga atcaaggctc cggatgattt ctgctgtttc
    11581 ggatgtaaaa aacagcagaa acccgctttc ttgtttttga gtcaaacata gagtggagca
    11641 attgtcatcg ttttttgtcg tccctttttg cagtgcgttt ttatatgagg tatgagaaga
    11701 tgtcccctta tctgctgacc cgtcgcgcct ttatcgcttc cgccaccacc tccgtactgg
    11761 cgacaggtgc ttttgccgca cggcccaata ctgcccgtgt cgtgccgggc aaactttcgc
    11821 ccaatgaaaa gttgaatgtg gcggggatcg gtgcgggggg aaagggcact caggatattc
    11881 tttctttcca gagagaaaac attgtagccc tttgtgatgt ggatgcgggc cgggcggcag
    11941 aggcctttta ccgcgttcct gaagcgcgcc agtttcagga ttaccggaag atgtttgatg
    12001 aaatgggatc agagattgac atggtcacca tttccacacc cgatcacacc catgcgcccg
    12061 ccgcctacag agcgatgaag ctgggcaagc atgtgtatgt gcagaagccg ctgacccata
    12121 ccgttgccga ggcgcgcctt ctgacgcgaa ccgcccgtga aatgggtgtc gccactcaga
    12181 tgggcaatca gggtcattcg ggcgatggcg tacgggatct tgccgaaatg ctctggagcg
    12241 gcgttatcgg tacggtcagc gaagtacacg tctggacaga ccgacccggt tcccggtggc
    12301 ctcagggaga accgactgcc ttgcctccgg cagagcccgt gcctgaaggt ctgaactggg
    12361 atctgtggct gggcgcagcg ccctggcgcg aattcaacgg gctttatgcg cccttcaagt
    12421 ggcgtggctg gtgggacttc gggtgcggcg gtctgggtga tatggcgtgc catgtcatgg
    12481 accccgcttt ttttgccctg aagctgggag aagccccttc tttcaccgtt gagcctgtcc
    12541 ggatggaaag tgccaacggg cagacttatc cgaaaaatat tattctgaaa tatgcgtttc
    12601 ccgcacgtgc cgatatgccg cctgtcgatc tgtactggta tgaaaatggg ctcatgccgc
    12661 cgcgccccga agggatccct gagggtgatg tgctgggcga caaacagaac ggttcacttt
    12721 tcatcggatc caaaggtttt cttacagcag gcgaatacgg tggcaatgcg cgccttcttc
    12781 ccgacagcat catggcggac tacaaaaaac cggatcagac gctgccccgg gtaagcggtg
    12841 ccaaccatta ccgcaacttt ctcgatgcgt gcaaaggaaa tgcgcctgcc gtgtccaatt
    12901 ttgattatgc aggacctttt acagaaatgg tgctgctggg caatgttgct ctccggctga
    12961 ataaagccat cagtttcagc acagaatcaa tgcaaatcac gaacgatgcc gccgctcaga
    13021 cgctgctgac caagccctat cgcaccggtt gggaattgcc tgtttaaaaa acatataatc
    13081 ccttaaattc aaccactttg aagaaaggga ttgtaatgaa aacaattttt cacctgacgc
    13141 tgctgctgct ctttgctgtc tctgtttccg tcgctgccga ggaagcgcct ctgaaactgg
    13201 ggctcatcgg gctagacact tctcacgtca ttgccttcac ccaggtgctg aacgatgcca
    13261 gccgtgaaaa tcatgtgccc ggcggacgtg ttattgccgc ctacaaagga ggcagcgctg
    13321 atctggaatc cagcgcatcc cgtgttgacg ggtatacaga acagctggtc aaagagtacg
    13381 gcgtgcagat cgtgtcttcc attgaggaac tgtgcacgct ggtcgatgct gtgctgctca
    13441 cgagtgtgga cggacgtccg catctggcac aggcaacccc cgtcattaaa gcggggaaac
    13501 ggctttttat cgacaagcct cttgccgggt cgcttgccga tgcccgggct attgccgctc
    13561 tggcaaaaga acataatgtc ccgtggttca gctcttcgtc ctatcgtttt tacaaaagcc
    13621 ttcaggaact gaaacaacag gatgtcggcg aagtgcgcgg tgccatttcc tacggtcccg
    13681 cctctctgga tccgacacat cccgaccttt tctggtacgg gattcatgcg actgaagcac
    13741 tctttaccat tatgggcacg ggctgccagc gcgtcacctg ctttgtaagc gatgattttc
    13801 atgttgccac aggggaatgg gaaggcggcc gcatgggcac gctctacggc ctgcgcaccg
    13861 gtgccgcacc gcatcgtgtc actgtcttcg gcagcaaggc agtcgcagat cagcagggca
    13921 gcggcgatta tgcgcctctg gtggcagaaa tcattcaatt cttccgcacc ggtgtaccgc
    13981 ctgtatcgcc ggacgaaaca cttgaaatgt ttgccttcat ggaggcggca catgagagca
    14041 ttcgacaggg cggtgctcct gttgctctgg atgaagtgat caggcaatct gagtgaccat
    14101 tctgatgttt aatatgatat tccaatacag tggtaaactg aatactattg acaaaccgta
    14161 attcctgcgg tatactttag tacccctgca aagtgtgcag gggcgccatg attccggcgt
    14221 tcaccatagg ggtgcccgcc atcagtttcc agttttttta cttaataagg agtcttgcta
    14281 tgaaacgcaa aggattcaca ctcatcgagc tgctggtggt tattgcgatc atcggtattc
    14341 ttgctgcgat tctcttgccg gcacttgccc gtgcccgcga agccgcacga cgctccagct
    14401 gccagaacaa cctcaaacaa tggggcattg tcttcaagat gtatagcaat gaagccccgg
    14461 gcgaaaaata cccgcccctg cagacctaca gagaccgcac caaatttgcc agtgccacct
    14521 gcaaccccgg tgctaacgcc gttaaagacg ctctcgcagg cgggccttcc gtgtatgcca
    14581 ttttcccgga atatctgact gacaccaaca ttatctggtg tccctcggat cctgaactgg
    14641 gccagcacaa agcgagcatg tttgcgaagc ccggtgagtt gcccggatgt atcgaagacg
    14701 gcggcacaaa gctcggctgg agccctgatg ccatcgacga cagctatttc tatcttggct
    14761 gggcctttga ccggctcgaa acctccgagc gcaccagtca gttcggtgtc gtaggcgccc
    14821 tgctctccgg cacctttacc ggtgatccct gggttcccgt gcagatcggt gccgctctgg
    14881 acggtcttgc ctccaaatat ccggctgaaa tcgctgccat gctcggcggt ggagatgtct
    14941 acggcgctgc agcagcggtg gacaaagaca ttgaactggt cgctccctgg aaagggatgc
    15001 atgcgggtaa tggcgaaagt gacacggtct accgcctgcg ggaagggatt gaacgcttcc
    15061 tgatcaccga catcaacaat cctgccgcaa gcgccatggc acagagcacg ctcttcatca
    15121 tgctcgacac gatgggcgcc ggtgccaaca ccaagtactt caaccacgtg cccggcggct
    15181 gcaacgtgct cttcatggac ggtcacgttg aattcctgcg ctacaccggt gtcagtgttg
    15241 ataatctgac gaattccgcc gaagtctccc agaagatgca gggctgccag tctcccgtgc
    15301 tgcccacact tgctacgctg gttgctgcct tcaactgaat ttccctgtag ttgctctttt
    15361 gtgcccggag tcgtgaggct ccgggctttt tatttttatc cggcgtctta aataagtccc
    15421 gtctctgtgc tttcttcttc ccgtgtggtt gtgccaccag tttgacaaaa ggcgaattgt
    15481 tcggtatagt gtgagatgtg ccggttgccg gtttgttttt taaatgattg ctttttatcc
    15541 cctgcccggc agggattctt ctatattttt caacagggag aaggcatcat gaaaaaacaa
    15601 gagggcttac acttattgaa ctgctcgtag tcattgcgat tatcggtatt ctggcggcca
    15661 tcctgctgcc ggcactggca cgggcgcggg aggcggcacg ccgcaccagc tgccagaata
    15721 atctcaagca gtggggcatt atcttcaaga tgtacgccaa cgagtcgccc ggcgaaaagt
    15781 ttcggtctat gccatctatc cggagtatct tacagacacc aacattatct ggtgcccttc
    15841 cgatcctgaa ctgggtcagc acaaagcaga cctttttaca aaacccggtc agctgacagg
    15901 ctgtattccg ccgggagggt cgcgactcgg gtgggacccc ggcgccatag acgacagcta
    15961 tttttatctg gggtgggcct ttgacaattt gacgcacagc gggcgggcgg gcgagcgaat
    16021 tcgcgattat tcaggcactg ctcagcggaa aatatacagg agatccgtgg gtaccaactc
    16081 agatcggagc tgcgctggac ggacttgcag agcagtactc agatctgatt gtgacactca
    16141 tgagcgggca gaatgatgcg gcggtcattg atgccgccaa agtcttcgat aaagacctgc
    16201 gtgtatcttc cgactatatg ggtgccggag tggggaacgg tcatggcgat acggtctacc
    16261 gcctgcggga aggaattgag cgttttctga ttacagatat caataatcct tcggcaagcg
    16321 caaaagcgca aagttccctt ttcattatgc tggacaccat gggcgccggt gcgggcacac
    16381 gctttttcaa ccacatcccc ggcggatgca acgttctctt catggacggg catgtgcagt
    16441 ttaagcggta tatcccgatg aatatcgacg ccattgccac ggccgctgaa atggctgcta
    16501 aaatgcaggc gtgcgaagaa cctgtcctcc cgacattggc aacgatggtc gacgctttcc
    16561 gtagtgcaca ggattaatct ttttacctga tcgtggtggc gggacagttt ccgattcaga
    16621 aagataagaa aagggaagag cacgtaagac tcttccctgt attttccggt gcagtggcgg
    16681 ggaaagtcag cagaaccgcc gcttattcca cttcataggt gatataatag ggtccataac
    16741 tgaattgata gaagagactg ccgtaggtga catcgatata gaaagcgcgg ctgtagggac
    16801 ctggatccga aataagcaca ccgcccgtat catagtgacc gatgggcaca acggctgctt
    16861 ccgccgtttc atagatggaa taaaaactgg agtacaccgg cggaagagtc gtgtcataca
    16921 caaaactgag cttacccggg aaggtgcccg gcagccattc cgtcccttcc cgcacagacc
    16981 agtgcaatcc ggcgaaggtc ggcatatgct caccgccgat gttgcgcacc tgaaccagtc
    17041 cctgccgttc atcttcaggc agatccttga ggttgcccag atcgataatg ctggactcag
    17101 gcgacagaag cggaggaaga aatgcaaagg cgggattggg gaaagaaaca gccttcatct
    17161 cttcatagcc gatatcgatt ccccgcggta ttgtgaaata tttcccgtca gcgccgccgc
    17221 caataacggg ttcaagcatg ttcaccgaaa ggtagacagg cgcactgagc ccggtaaaat
    17281 cgagctgaaa cagattaccg tagtcaccat aatcaagcgc tctgcctgcg gaaggtgtct
    17341 ccaaaataag catattgcca ttgcgtgaca tgctccagtg tgccacgagt ccgccctgct
    17401 ccgccggaag gagagaagcg gaccagggac tgccgtccga ggcattgagc tcgaagacca
    17461 gccgcgtaat gtttctgggg atataatcgg catagaatcg tacggaagcg ctgccatcgc
    17521 tacgaatgcc gtctgtatgc gtgccgatct gccccaaccg tacatcattg gcaaattcaa
    17581 tagacggaat ttgagaaacg tagaggctct ctttcagagg cggcgtggca acatccaact
    17641 cgaaacgcgc ctggatatcc atgctggact cttcattcag ggtgacatag gaaagcacaa
    17701 tatgtgattt cagatcggag acaatggtcg gagcgttttc atcgtccgga tctttcttgc
    17761 accacttcat aagctccgcc ttgacgggaa gcgtaagcgg attgccgtcg gctcctacag
    17821 tgccggggat gcgtttggtc gacgttgcat aataatgccc gccgctttca tcggcaatct
    17881 ggatcaggga attggcataa acttcggaac cccagccaat ggtaaatgta cgggttcggg
    17941 tggctctgag aatttctgtc aacgctgtaa tttcaccggg gggcgttgtc cgttttccgt
    18001 cggtaatggg aagcagaatg cattgaccca aattgtcaaa gtcgggcatg ttgccgccca
    18061 gacggaacag cgccagcgct ccttccgcaa tcgccgtata aaggggtgtg gctccgttat
    18121 caacaacctg cagcgtattc aggcggtacc ggagaatatc ctgattgctg gtgaaggaag
    18181 cgccgcttgc cttactgtca taaccggcgg gcgctccggt aagaatacgc atggcaccgc
    18241 cccaccaggg attgcgttca ttgaaaacgg caagccctac ccgataatga ggcgggactt
    18301 cgtccagaat accgcctaca gcctcgcgat acagggctgt aagcggatct tctccgggag
    18361 cgggctgcag ctgcccgtca tccaccagcg cccgtgccgc ctgtgccata cttcctgaaa
    18421 agtccagcag aatcagcatg gcaaaactga gcgattcgtc tttctttaca aaaacattgg
    18481 tttcggcaag atcgaccggc aacgaatttt cgagaatatc cacacggggc gttgccaggc
    18541 ggtacagcgt ccggctatcc agcagatcgt ctttaaacat cgggaaagtg cgctgacgga
    18601 tatccctgag cagcacactg gcgcgaatca gagacggagg tctgaactgc gggctggcac
    18661 tttcaatact cagttccgca agatccagtt ggacagtcac ttctacagga accacaggag
    18721 acgcattggg cggcacgtta tctgccgtga tcagcaaggt ggcggcggca ccggtacctg
    18781 ttatactgct gcggtcaatg gcaacactga caaactgcca gtctttgacg gcacccgctg
    18841 tacccatgct tctgccccgc gccggttcgg caaaaagcca tccgggctga ctggaagtca
    18901 caatgtaatc caggggcacc ccggcatcac ccagatttgc cacttgaaat gtgagggttg
    18961 actgggaacg tccgaaattt aatttgggcg gctgcacccc gattacaatg ctatagggga
    19021 cgcgcacatg cacatcaaga aaactcgtag gcgcttccgt atcgttcgag cggatgggca
    19081 gcctgtacgt gccgcctctg ttgtccagac ccgtgcggtc ggtggtcaca gtgaccttct
    19141 gcattttccc gggtgcaaga tctccctgca agggggtaat ggatgaaatc cagtcgggca
    19201 ggtgatcgtc agcaacattc caggtcagtg cggcattacc cagattttcc actgaaaact
    19261 gagcgctgtt ttcattgata tccatcataa taaaggggat attagttaca tccacccctt
    19321 caccggtaag actgatttgc gggaagcggc gcgcctgagc acgaaccgtc acttccaccg
    19381 gctccgcatc cggcagggaa ctctgcactt tcactgtgtg tgaaaagttt tcttcgccgg
    19441 ccggcgcaag ctcccggttg atggcgaaag aaacggtttc cgtaaatgca ccggaaaggg
    19501 atccgcccgt ggacgaaatg ctgagccacg ccggaaaatc ggaagtatct atagaccagt
    19561 taatagcgcc cggtccctca ttccacagat tgatatcttt tttgttttct ctggagccga
    19621 agttaagatc ccgcggctcc acttcgagtt tgtctttttt cggatcaggc gtgcgattta
    19681 cagatacgcg caccagtttg agcacagcac ctgccttgaa ttcaagcgtt acctgtgcgc
    19741 taccggcggg ctggcgggtc gtgtccgcca caagggtcac cgtaccggta tttgtaatcg
    19801 cagcagattc agggcttatc gtgagccagg gagcaggtga ctgcttctga acctgatagg
    19861 tgaaaggcgc gtctccggca ttggtgagcg tcagcgtggc tgttgcggtt ccgttgagat
    19921 ctccaaaatt aatttcgtcc ggatcaatca gaaaatctgc cgccttgcct acttccatct
    19981 tcagcgaaat ctgttcctgc tgtcctgtcg gtacatggct cagcttaaga acagcggaat
    20041 aattgccggg agctagacct gcgggattgg ctgtaatgga aagatccaga ggcttcgtac
    20101 ggcttgcttg cagtgcggca ggtgtgacgg aaagccaggc gtttccgctg ccatactcaa
    20161 tttccgaagt caaaacaacg gcttcaggcg attgggtacg cagggaagcc aaaagcgttt
    20221 tgggtgcggc aatcagcccg aaatccagtt caagaggaga caggctgaaa gcgcctacat
    20281 caaccgtaac cgccagactc tgccttccgc cgttggattc aaaaacaagt tcaccctgat
    20341 agggttccgc ggcgggcgtc agcatggcgc ggtcaacagc gatggaaagc acctgtgcac
    20401 tgcctgctgt tgtctcgccg gaagaagggg ttaccgaaag ccagggagat gcagacgcaa
    20461 cccgtgactg ccagaggagc gtggcagcac cctcgttcgt aaggtcaatg gtttttttgg
    20521 tttcgctgct tttgaagtgc agagtaacgg gctgaatggt caaccgcggc tgccggtcgg
    20581 gttcaaaagt gaccgatatt tcttcatcgc cgccgccgct tgtattgaat acggtaccgc
    20641 gcagtctttc atctgtaccg ggcagccggg atgcatcaag aaagagggta ataatatctg
    20701 tcttcactgt tttaccgctg atctgtgcct cggcactgcc ctcttcaccc aggcgcaaca
    20761 gcagccaggg ctgacctgca cgggctgtcc aggacaaagt tccactgcct gcattgatta
    20821 cacttatctg ggcggtggac tgattgatac tgagcgatac ggatcccggc tgaaccagca
    20881 gttcaccgga gaacgggcag ccggaaagca gcacagccgc aaccacacat accagccatt
    20941 tcatcatatg ccgtgcagac attttcatgt ccatgatcct ttcatcagaa agcaggcgca
    21001 gagttgaatc agagccctat attgatcaaa gtcagttcgt caggatacag atcatttttt
    21061 agccggatac gggcgctgta gttgccggca gccagagaag ccggatcaaa aaacagcgtg
    21121 cagtgaagtg tttccccagg ctgcacccga ataggttcat cgctatctat cagatcggca
    21181 accgccggat ctccttcgag gctccaggaa aactgatcgg gctgcagagg agtacccccg
    21241 ataatctcca ctgaaagagc cgtaatgtta aacgtatccc agcggttatt ggtgatcgta
    21301 accgcccggg cagtatcacc cgcagcattg aaagacagag actccggctg aagtatccgt
    21361 ccggggcgcg tttcgtcatc cgggtaggga tcatcgaagt cgggaagtcc gtcgccgtcc
    21421 ctgtcccagg catcgggcgc ttcgggattg atgccgggcg ccgcaagatc tgcgatactc
    21481 cgtgccggtg cggcaagatc ggacgcatct ccaatccaca aagaccgatc gggattaagc
    21541 ggaccggacg gataggtaaa gtacaccgta ccggacggct ccgtattgga tgcgggcgca
    21601 taatacacgc tgttgtccac gcgcataccg agatagaatt ccgccggaat accggcaacg
    21661 gcgcaggcat ccacaaaggg aagtaaatta tcgaaccgaa tgcgcagcag attgccggac
    21721 gacgcatacg gcaacggatt ctcttctgtc gtcagcaact cgtaaacgtg attgcccttg
    21781 ctgatcatgc gccagttggg cttcgatccg tcagagaaat agaggaggcc ctcctgatct
    21841 atttccacag tcagtgcagc cgcatcaaaa gcggcacgca ctgccgccgg cgcctgagca
    21901 tcaagctgcg atatgaagcg catccggaag aagttgacat tgcgcggcac ataatccgca
    21961 cggacgtaca cctccgcccg ggctcgctcc gcatcggatg ccgcagtccg gttcaggctg
    22021 atgcctgttg tatacagaga cagctgaccg ccgtgaacgt cgccatggta gtacatgccg
    22081 tcctcttcaa acatggcgcg tacatctttc ccctgaggcg tgcgccaggt tgcgataatg
    22141 gaatatttgc cggaacgctg caacggcgtc acataagaca gcactatctg attgctcaat
    22201 tcattccata tgcgcccggg ttcatctctc aaggacaggg aaagctgctg cacgtctgtg
    22261 cccgcatcat tcagggcaag aaacagctcc gcagcaccgg atccgtcgct ggacaggata
    22321 tccagaaggg cgagccccgc tttgccgggc gtattcagta aagcaggatc gagtgaaaga
    22381 agcacctgca cttcctcgcc cggtgcaagc gtaccatctg caggcgacac cgtgcggagc
    22441 cagtccgcat gattctggcg cttcacctgc caggacaggg gatcttttcc tgcattcttc
    22501 acaataaagg ctgccgttga cgagaccgca cctgtatcct gcacgggctt cagcaccagc
    22561 gaacggcggt gcccgagaaa agtcacgagg ttggcggcat tgccggcacg gtagaggtgc
    22621 ccgccggttt cgtcagcaag ttttatcata tcgggataat tgacggcgct gcctgcgctg
    22681 taggtaagcg gatacaaacg taccttctgg ttgcgtgcgg ccgtcacaac tgcggctgca
    22741 tcctgtgtcg aagaattatc tttgccgtcg gaaatgaaaa caacagagcg aagatccgca
    22801 tcgtcaaagg gcagcacatc ttcggcatct tccgccgcca gccgctgaat ggcttctatc
    22861 agagcatcat acacatcgga agtaccgtgc agcgccgggg gtacagaaaa acgcttaagc
    22921 gccgctttca gcgaagcacg gtcggtagaa aatccgtgga tcatgcggct gcgcggctga
    22981 cggtcgttat gatacatcag agccacgcga tagcccggcg gcagatcatc caggaacatg
    23041 gaggcacttt ctttaatctc ctccagcacc tcgccgggaa ggcgcggcga ttcgggatcg
    23101 cccactccgg ctttgtacat actgcctgta tagtcaagca gcagcaccag attcaccttc
    23161 aggttttcgg ggccattcac ctgcatggcg gtctcgttca gatcaagaac ttcgccgtct
    23221 tcctgaattt caaagctgag cttgcccaga tcttcaggcg tacgggtggg aatcacttcg
    23281 cccagcatat cacgcagcag cagtgcaaag cgcatgagat agggcggacg actccggtta
    23341 agcgcccctt ctatcgtcat cggtgcccgc tccactttaa tctggactgt ccacggttcc
    23401 acagcggcaa taggcccggt tccttcctcg tcccaggctt caatggtaag gttccggtat
    23461 tccagatctt ccttcatggc gctgcggtca acggtgacca cgatctgctg ggcatccacc
    23521 atgcgatcca cactggcaag ataaaatacc tgattcggtc cggtggtgtt gccgcgcatg
    23581 gggcttacat ccacaacaag cggcgactcg gcattttcat catcatgcgt gatccggaaa
    23641 tagagccgtg aatccaaagc ccctgcatta aaaacaaaaa acttcttgct cgtctcttct
    23701 ctgccgaagt ccagcagcgt catataggta ggcgtgggcc gaatggcgtg aggctccggc
    23761 ggatcggaag ccaccaccac gggcaggctc accacttcca gcaccagcgg aacaatcacc
    23821 gtgccatcgg caccgggcac gtcaatgcgt aattcgtaat tgccgctgcc cggaatcgct
    23881 gccgccgcat tggaaacgct gacaagcact tcgccttcct gtccgggctg caatgtcccg
    23941 agcacaggcg taatctgaaa aatatctgcc gccacaccgc cgataccgtt gacactgagt
    24001 gtccaggaaa ggcttgtgtt tcctttgttt ttcaaagtga ggcgggacac aggctgctca
    24061 gctcctgagc ccccctgaaa ggcagggctg ctctggtaat agaccctgag tgtttcagga
    24121 tcaacgccga gagtgccaag cggtttcgaa aaagtaaagt acacgacagc aaaatgcccg
    24181 ggcgcatcga cattaaaaat cttcagcccg aaatcacccc gctcacgtgt ccactctaaa
    24241 cgaacagtag ccctgctttt tccggaaagg cggccggggg cgggagtaag catcatgtcg
    24301 ccgggcagcg attcttcctg ccctgcaata ccttcaaaat aacgcacctg ccaggtaaca
    24361 gcggcatccc cctgattttc cagttcaaaa gaggcattcc gtgcatccgg tttcagataa
    24421 accttggaag gcgtcgcctt cagcagcgga gcaacagtca gggaaacagg gacgatctgc
    24481 tgcttgctcc cgcaaatgaa gcgcaggccc acattgtcgt atagtcccgg ctccagcgca
    24541 ctgcggttgg cggtgacaac cagattttcc acgcccggct tgagagagcc cgcatctttt
    24601 tcgcacgcca gccaggacac tgacgctgtg gtccagggtg cgtccgcatg agcgcgcatg
    24661 actgtatcca cagaccaatt taaagcccgt gtcccttcgt ttttaagcgt tacccgacgc
    24721 tcgaaaacag tggatccaaa ggaaatgctt cccggctcaa cgctgaattt cggcgtagcg
    24781 gtacatccct gaatcaccag aacaagacac acaaaagccg ctaggctgac tgccagatcc
    24841 aatcgttttt tcattgcatc aagacgcctt tttgcttttt acaatcgaat gggcggcctc
    24901 cccacccggg gcgggtctgc tggctgcgcc gatcactcaa tccagaaatt cgacgacaac
    24961 tccttcctga aggagggtat catagacggg agcaaagccc ggcaaaggat gatcatcctt
    25021 atcgagcgta ttgagtgctg acagcaagac cacattgccg gcgccaagac cgccgttcag
    25081 cacattgtcc tgcagcgctt tccagtccgc aatcacattg tccacaccgg aaagatccag
    25141 cacttccaga tgcagcagcc ccgcaagcgc ttcaatttct acaatcctgt tgcctctcaa
    25201 atgtacatgg gtcagctttg tcagagaact cagcggatcg atattccgca ctgcattgtc
    25261 gttaaggttg atttttcgca gattcttaca gtactgcagg ccctcaagcc gctcaatatt
    25321 cagtgcggcg gcattgagtg tggtaatttt ctccaggtcg gcgcgggtga gaaaaaggga
    25381 cagcggtttg ctgagttcct gacgaatggc agcctcgagc gctttatccg ggacaaatac
    25441 cacaggcgaa catgccgata gcccgatcag aagcactgcc gcaattcccg tataaaccag
    25501 aacctgtctt tgatggaagc ccatagtctt ttccttcctt catgtctttt tatcaggctg
    25561 cttctccggg caacatatca cagctgtccg tgacatctca ttgaccggtg agagatacta
    25621 tttcactcat aaaatgcaac ggaggcgccg agcggaaaga agtcgcctcc ggtcgtctta
    25681 ttagcggttt tccggcggct taatggtgca cttgtccgca atatatccgg aaatcggcgc
    25741 tgaaccgccg aaggcaccga taatgttatt gaaaagcgtt gctacgacac acacaaaatc
    25801 ttcccagctg ccggctttct ttaccgacct gcgcgtcacg ggtgccagat gtttcataca
    25861 gatctcttcc tttgtttcca gccgatgaac tcggtcttgt attgccatct ccaaaaaaaa
    25921 agagacagaa ctacattcca ttgtatccta tagattactc gattttcctg aaaatgtcaa
    25981 gaatttattg ttcggaaagg gaaattttta taaaagtttt gtaccggaaa ctcctgtgtt
    26041 tatgaaagaa caggaagatg aaactatatt tcacggaaag attgaatcag aaagtcgcca
    26101 gatttgaaaa aagaaaccca tcaggcgcca atattcgaac tgagggtcag catgggcagc
    26161 agcatggcga tcaccagaaa accgatgact acccccataa taacaatcag aagaggctca
    26221 aaaagcgccg taagcgcttt cacagcacga tccacctcga ggtcataggc gtcggcgata
    26281 cgtctggcaa catcgccgat ccgcccgctc tcttccccga cggcaaacat gctgaccacc
    26341 atcggcggaa acaatggccc ttcacgcatc gtttcgctga ggctttcccc ttccgttact
    26401 ccgttgtgca gctccgcaac acgatcacgc accagcagat tgctcatggt atctgccgtt
    26461 atcttgagcg aagtcagcac cggcactcca ttgtccagaa gcgtgcctag cgtccgtgaa
    26521 aactttgcca tttcatattt aataatcagc gtgttaacgg ccggtatttt aagcgcccag
    26581 ccgtcaaact tgcgttttcc cggctccgta cggtaataac gtatcagcag cacaacggtt
    26641 cctacgatgc ccagcagcac cgcccaccac caggtcccca tgaaatcaca aatcttcatt
    26701 acaatgactg tgggcagggg aagctgtgcg ttgaagtcct caaaaattgt aatgaacttg
    26761 ggaaaaacaa aagaaatcag aataaaaatg gccgcggagc cgacaatcag cagaaaggta
    26821 gggtagatca tcgcggacac cgctttaccc cgaagttcct cttcctgttc accgaaggca
    26881 cagatacgcc acaggacctc ttcaagcatg ccgcctgttt caccggcacg gatgagactg
    26941 cagtacatgg aaggaaagac ctggggatgc ttttccatgg catcggcaag actcgccccc
    27001 ttctgcacat cgtcccggat ccggtcgaca atgcgggcga gtttagggtt ttctgtctga
    27061 tcgaccagcg tacggagcgc ccgggtgatc atcataccgg actcgctgag attcgcaagc
    27121 tggcgcaaaa agatattacg atccttcaca cggatccgtt tcatagcctc ccgaagcgtg
    27181 tccttgcttt cttcctccac ctgctcgtcc acgcgaatgg gaaaatatcc catttcccga
    27241 agccgtgcca cagccgcccg cttgttttcc gcttccagca cgccattcac gacttcgccg
    27301 gggccttttt tcacttcata ttcatacctg ggcatatact aggcatcccc ctgatgaatg
    27361 gggtctgtgt cggcagtcag gcgcagcact tcttctgccg ttgtgtcgcc gctgtacacc
    27421 cggcgccagc cggcatcgcg cagcgtcctc atgcctcgct tcatggcttc ttttttaatg
    27481 atacccgcat tgacacgctg cagcgtcatg tccttgatcg cctcagtgaa aggaagcatc
    27541 tcataaatgg caagccgacc cttatacccg gtgtatcggc attgttcaca gccttttccg
    27601 cgcataaagc gcgctccttc aagatccttg tcgctcagat caaattccgc cttgagcagt
    27661 tcccgatccg gctcaaaagc ttcactgcaa tagctgcagt ttttccgcac gagccgctga
    27721 gcgaccgagg cgatcagcgt gctggcgatg agaaacggct cgattcccat atcaatcagg
    27781 cgggtgaccg caccggcact gtcattggta tgcagggtgc tcagcaccag atgtccggtc
    27841 aggctggagc gaatcgccat ctctgccgtt tcataatcgc ggatctcgcc gaccagcatg
    27901 atgtcggggt catggcggag catggagcgc aagcccaccg aaaagtcaaa accgattctg
    27961 ggctgtacct gcatctgcga aatgccggag agctggtact ctatgggatc ctctatggtc
    28021 agaattttcc ggtcaacctg gttcaacttt gccaaagcgg cataaagcgt cgttgttttt
    28081 ccgctaccag tgggaccggt cacgagaata atcccgtgag ggcggctgat aaagcggttg
    28141 aactgctcca tatcttcggg aagaaagccc agctgatcca tcgaaagcgc catggatgaa
    28201 cggctcagaa ttctgatatt cactgtctca ccgtggggcg taggcagaat cgacacacgc
    28261 aaatcgtaac ggttttcccc gaaattcgcc agaattttcc cgtcctgggg aagccgcttt
    28321 tccgctatgt tcaggtttgc catgattttt acacgggaca caatagcggc atgaaacccc
    28381 cggatggaag gcggcgtcgg tgcctgatgc agaatcccgt caatgcggta acgcacccgc
    28441 agactgtctt caaaaggctc gatgtgaata tccgtggtat ccgac
//
LOCUS       DAOPWB010000022        28453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1621_length_28453_cov_6.186386, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000022 DAOPWB010000000
VERSION     DAOPWB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 28453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28453
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1621_length_28453_cov_6.186386"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PKN92_03580"
     CDS             complement(<1..861)
                     /locus_tag="PKN92_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNZ47905.1"
                     /translation="MNNFREQVNALTNALDEVILDKGPVIKLALTAVLSGGHVLLEDV
                     PGVAKTMLARTLAVASGCAFNRIQCTPDLLPSDITGVSIYNPKTQEFSFREGPVFSQI
                     LVADEINRATPRTQSALLEAMAEKQVTIDGHTYQLEQPFIVFATQNPVEHEGTFPLPE
                     AQLDRFMMRLSLGYPGLLAEAKLLENTRLTHPIDAVKKVCDPKTLLKMQAAIKEVHFH
                     EKVRHYMLKLINRTRDCTHLSLGASPRASMMLFRACQAFAAIQGRNYVLPDDVKSLAR
                     PVLEHRLLLHP"
     gene            complement(884..1864)
                     /locus_tag="PKN92_03585"
     CDS             complement(884..1864)
                     /locus_tag="PKN92_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ47906.1"
                     /translation="MKYVQFGSTGLEISEICLGTMTFGNEADEAVSFSIMDYAASLGI
                     NFFDCAHNYNKGLTEEIVGRWLKGRRDEFILATKVFFPYGGGRNDQGLSRRNILASLE
                     RSLRRLQTDYVDILYLHHWDENCSLESSLAAVNTLVEQGKVHYLGISNFSAWQVATAM
                     GITRIKSFAPVSCIQPMYSLVKRQAEVELLPLAEYERLAVFPYNVIGAGLLTGKYLTG
                     GSGRLKEAEMYRQRYANPQYEDVTRRFIAYASQKGYSPAALAAAWVKSHPSVSAPIIG
                     ARTIEQLQDTLGCLDIVLDPEERAALSALSPEPPHATDREPLAAMKSRGW"
     gene            1952..3424
                     /locus_tag="PKN92_03590"
     CDS             1952..3424
                     /locus_tag="PKN92_03590"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNZ47907.1"
                     /translation="MKQQSKANTEKKAQVFSALYGDCQERAFEQTVLQETPESDLQAL
                     WQGLILPTTQNTVEGHPFRIIAPGWWNKLKGPDFINAQIEFNGKLITGDVEIHRHNRD
                     WFHHGHHEDPAYNNVVLHAVETEGAPAAVTQSGRTVPTWVFPPTSQSDLDMKRQISRQ
                     QCGECAKSYAFRNPALLEHFLDIAGDWRIREKERRIRKRAEEVGFDQTVYERVLESCG
                     YSAFKKEFELLGKALPYERALQLGREDKMALEAAYLRLGGFLPVEWMVPDTKAPAHFT
                     RLVQAYEQYLHPMESLGISWKKQAVRPANRPERRLAGMAGFMCKVTDRGLERQLDFLW
                     RQPVSVRERIDNFCALFGKNLSYWANHYTWGGKELGKAAAVLGEGRARSILGNIFVPA
                     GLFLARQDAAGSLLENNVHELFRTMKAEPKNNVYESMLKWIVPPGVKIKMNFRRQQGL
                     LQLSADWCERNPSCHNCILPVFLQVIDEGAYQSVFDSGLL"
     gene            3605..5227
                     /locus_tag="PKN92_03595"
     CDS             3605..5227
                     /locus_tag="PKN92_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ47908.1"
                     /translation="MRLNNLQKQSSIGGMLILLFLTSGCFSNVTREAGVLQVGNGAEV
                     QELDPHIVTGVTEHRVLTSLFEGLTDCDSSTLEPVPGVAENWEVSEDGLHYRFTLRKD
                     ARWSNGDPVTAEDFSYAWQRILSPALASEYAYMLYCLKNARAFNEGTITDFAEVGVCA
                     VDSWTLDVFLDNPTPYFLSMQNHYAWFPVHRKTIESHGSMDERGTRWTQPGHLVSNGP
                     FLLQAWLPNEILSVRKNPFYWDKEHVQLSGIDYLPIDNLQTEERAFRSGLIQLTSTIP
                     LQRVESYRKKHPELLCLLPYYGSYFYRLNVTRPPFDNADVRKAFALSLDRQEIVDNVL
                     KSGETAAFSYVPEGAGDYTCTHPLRFDVAKARALLASAGYPSGRGFPPVEILYNTSEA
                     HRIIAETVQRMWHDNLGVEVRLLNQDWKVYLSALNTLDYSISRSSWVGDVADPINFLE
                     CFQTDVGNNRTGWSSQHYDTLIKEAHQCPDKEKRIALLQAAETVLLDECPIVPVYFYT
                     WKFLKAPEVKGLTPNILGYIRWKDLSLDTAEG"
     gene            5231..6148
                     /locus_tag="PKN92_03600"
     CDS             5231..6148
                     /locus_tag="PKN92_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ47909.1"
                     /translation="MAAFITKRLLEFIPVLLAIITITFFLIRLAPGGPFDTEKRVSPE
                     VLAQLEAHYQMDRPLHAQYFQYLKGLIRLDFGPSYKKPARTVAQWLLLRLPVSIELGL
                     YGLAFALFLGLGAGVLAALKPNSWLDHTVMSVAMLGICVPAFVLGPLLVLVFALWTDW
                     LPVMGWISPAHKILPSITLGAGYAAYIARMARGGMLETLAQDFIRTARAKGMKESRVV
                     LKHGLRAGLQPVVSFLGPAVAGLLTGSFVVETIFMIPGLGREFVESAFNRDYTMISGA
                     VLVYAVLVMSLNLVSDILLGLLDPRIRGQ"
     gene            complement(6174..7178)
                     /locus_tag="PKN92_03605"
     CDS             complement(6174..7178)
                     /locus_tag="PKN92_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47910.1"
                     /translation="MNSSILVRLRPTLLFSKKQRILCYRPAEDTALSWLRTRRYNVEG
                     PEPEKKAGSTLQNVRRYYDLVLIDQIRDAGALSEQNKEILRDGFNYVRPGGRMLLVSS
                     FSEKAGAVPADKITEEVGIVLKGLPVLQIKPSKVSVTHGHVLHLIQKSGVYKSQLPVC
                     YVDIQEEFNAICTALHKETLLGLDVETTLEEPRTLCTVQLASPHEVYVFDALKITDLE
                     PLKHLMENEEILKIIHNKDFEQQILGEKGIAIHNIYDTLVHARKHYKKTDVGSYRLDD
                     VCVRALNIYLDKHYQTSDWTRRPLMPEQIDYAAADAEVMIRLHRHFEPPPPPENLELF
                     "
     gene            complement(7255..8253)
                     /locus_tag="PKN92_03610"
     CDS             complement(7255..8253)
                     /locus_tag="PKN92_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ47911.1"
                     /translation="MQYKALGKSGIEASVIAIGTWVTGGGESWGGVDDAESIRSIDAA
                     LDQGINFIDTAASYGWGHAEEIVGQAIKGKRDKVVLATKCGIWWQDNRGSFHYHLNGK
                     DTFISLRPDTIAEEIENSLQRLQTDYIDLYQVHWPAKDPENTPIPETMGCLLKLQEEG
                     KIRSIGVSNVSVEQLECYCACGDIASDQFRYSMLYRDPESDILPFCQKNNLATLTYMS
                     LEQGLLTGKVGLSRSFKEGEFRTNTDWNPWFKAENRPKVLDMLAGWSDLTEKYECSLA
                     QLVIAWTLVQPGVTHVLCGMRTVAQALDNAGAGNVALDKNDAERMRRDALALGDPV"
     gene            8493..8765
                     /gene="rpmB"
                     /locus_tag="PKN92_03615"
     CDS             8493..8765
                     /gene="rpmB"
                     /locus_tag="PKN92_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNZ47912.1"
                     /translation="MARVCQYSGKRPQVGNRVVRRGKAKREGGIGQNVTGLSKRRWNP
                     NLQTIRVLDENGTVRRVKVCAKYIKAGKFVKAPRGTHAAWKAAEGK"
     gene            8847..10031
                     /locus_tag="PKN92_03620"
     CDS             8847..10031
                     /locus_tag="PKN92_03620"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47913.1"
                     /translation="MAQLTDLTTDSLVELQIDSVAHGGDGVGRLSGLVFFVTGAFPGD
                     RVRARVTHKGRRAVWCSLEEIMESGPCRALTGISDTLACASSCLWHDFLYPAQAEWKC
                     RIVEDNLKRIGGLSAEIKWLEDPQLRTGYRTRAVFHGDGKVLGCYARRTREIKTATPC
                     PSNHQRLLHAFQSLQSAGIYGDVQITVNPEGDEVLVHLQKDSPEARLRFPLVNTGDDK
                     KRHHFVFDGVPVVNNSFSQASLLLNRILRKKVDTCIAGAGSLLDLYCGSGNFSLHYCD
                     SAKVLGVDSNVHAIKAAQKHAPGSYECGTETLMKKLIQESVWDVILLDPPRTGAQQLI
                     HCLKEARAKRIVYISCDPATLARDLKILSSSMRWHLKEVTAIDMFPGTPHVETVTLLE
                     TD"
     gene            10068..10643
                     /locus_tag="PKN92_03625"
     CDS             10068..10643
                     /locus_tag="PKN92_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNZ47914.1"
                     /translation="MTISKICVYAASSNVIAPVYKAAAEELGVLFAEHGKTLVYGGGN
                     VGLMGVCARAVHTRGGKVIGVITDKLVDLELVYEKADELVITTTMSERKRIMADRADA
                     FVALPGGLGTLDEIIEILVLKQLWYHHKACVFLNTNGIYDSLFRFLDDLTRDGFSPAS
                     HRSFYHVCSTPAEVMSYLEAFEPEPAHEKWF"
     gene            10778..13507
                     /locus_tag="PKN92_03630"
     CDS             10778..13507
                     /locus_tag="PKN92_03630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNZ47915.1"
                     /translation="MATSDSKDKKELSFFILFLVLGLFFLSGACSLVYQVVWTRKLAL
                     LFGVTSHAVSTVLSVFFLGLGAGSFAGGRIADRSRHPLRLYGLFECIVGVSAVLVLLL
                     LSVSESWIPTALRLFDFSRTTGVLLRAILSFLLLFIPVSLMGATLPLLTRFAVRAQEK
                     TGFHVSILYAVNTFGAVAGCATAGFVLIASLGFSKTVYLAAALNIGAGLAAVLLSLLK
                     EPLTQSAVPENDNATPGCGSRGGAGPMRLLIFATALCGFCGLAAEVVYTRLLSIVFLG
                     TTYAFTTMLTAFLCGIGAGGGCAALIQRKMRKENALLLVGLISGGIAVAMLWSLPWIA
                     GLPDRFLDLQRSTGLVWDATLRGTFFLAFLTLFPMTFLFGVAFPLVIGAGGLWRKSVG
                     SLVGLMNGANTLGGVAGAMAGGFFLLPLLGSQNSITFLAVLLLVNGLALIVLGRTSGK
                     SIRVSIASILLLCFFVTFLFLPADVNLALNKGYMPRDHEVLYMKEGTEGTVAVSGPVG
                     AQHGEDRVLWINRVQATTSIERGVKMNRLQGVLPLLFDRTCERVLFMCFGSGITCGTL
                     ALSPFEQIDAVEISPEVLAVAPYFDKDNLGVLYNPAVSFHIDDGRNYLLRTDRKYDLI
                     TFEPMPLALAGVSTFYTEEYYRLCRKCLASGGIVSQWIPLHSNDPEMVRSLVFTFISA
                     YPEYCAFFVNADLFLLGSDMPLRLNYAKAKERLKNSVLADALEKSGLGDLAEIMSCYL
                     FDKQALDAYASGGVSMSDDLPWAEFTAPRLVYARLVPEAVDTLLPFISNPLEAVIPGS
                     ITAEERQRLERRCHSRRLDFPALKEYYGGSMFDDRAADGFAASLEADPENLNARYYLK
                     TIVLAQGERFLQWDDWERVIALTGKALRFMPDDKDLTELYQAAEQKRDLSAGR"
     gene            13551..14660
                     /locus_tag="PKN92_03635"
     CDS             13551..14660
                     /locus_tag="PKN92_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNZ47916.1"
                     /translation="MKSFWKRGRLRAAVACVLLIVFSGVLLKKTGRDENRAPDFAPAT
                     PPFSSQSYIQSGSCASLPTESIQVFFTTPGIEAAPPILHAFLQLIDSASVQICCAFYD
                     LENEDVARALINKHHAGVQVAIVSDSNYDRRKAVKQCLRAGIPVVFDTSKAFMHNKFC
                     VVDGRFVWTGSTNITQNGFYKNNNNALLINSQELAVNYINEFEEMFLHRLFGAGSPKN
                     TAFPLLSVSGISIECYFAPEDGAAQRIIDCIQTAKTSIDFMAFSFTSVPIAEAMAQRM
                     RHGVKVRGLFEKRDADSQHSRYRFLKENGAEVHLDINPNNMHHKVIVVDREHVITGSY
                     NFSKSAETRNDENILILHSRDIADCYVQELERQVS"
     gene            complement(14679..16037)
                     /gene="rimO"
                     /locus_tag="PKN92_03640"
     CDS             complement(14679..16037)
                     /gene="rimO"
                     /locus_tag="PKN92_03640"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNZ47917.1"
                     /translation="MTVPSFQCGIAVLGCDKNTADAEHFAGLLDLDAGREITVLCPGF
                     PEDDLPHLDAVVLFTCAFIHDAKEESVEAILRWVNHREAFGFPRQIYVAGCLSQRYAD
                     ELKKEIPEVDAWVGISDLQKLKALLLEKAPRSCCAVSSCSSGSRKRLDNKPYAFLKIG
                     DGCDHACSFCIIPAIKGRLHSRPSQEILDEAKVLIESGVRELNLVAQDCTAYGRDLYA
                     EYRLQHLLADLCAIPGDFWIRCLYCYPGGITEALLEEITAQPKIVPYLDIPLQHTAPK
                     VLKAMQRPAHALNIGDLICQIREKVPGITLRTTMLIGFPGESLTDHRHLLRFMESMPF
                     EWLGAFPYSREEGTEATLMKKQLRRDTITRRCNEVLELQAFITEAFNMSRENKTVRAL
                     IEGYDDERNCWIARSASEAPEVDGVIFVESKTPLKQGSFIDVRLMRTELYDMVARPAT
                     EI"
     gene            complement(16126..17289)
                     /locus_tag="PKN92_03645"
     CDS             complement(16126..17289)
                     /locus_tag="PKN92_03645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ47918.1"
                     /translation="MFTGPSFVAFSGVFTLGVLFSILGSVKLKLAETLNIDDTKVGSL
                     ISAMMFTCLIAVLIVGPLTDTVGYGMVALIGFGLACVSVLVLASARNYTTALIACLVL
                     GIAAMCANTIGNTLGPTVLFEGQNAPRASNLLNVFFGIGAFITPLIIALLLSNMGYKK
                     TVSLIGAILFLPIILVALTMKQLPQPEAGFNVAKTLGLLSNPVVLVGGLALFCYIALE
                     SSMAGFVSTYLKSHQFSDEKATSFLSGFWIALMVARLITAIFLTGLDPAVLVPVLALI
                     AALAIGLMIIAPNPATGVFGTLLAGFSFGPVFPTLVGVCFGKTDAINQGTAGSVFGLI
                     FAIGLFGGIFIPPIIGNYTAKTSIRQGLKIALGVSVALMIIGTVLWRFVPNVS"
     gene            complement(17386..19332)
                     /locus_tag="PKN92_03650"
     CDS             complement(17386..19332)
                     /locus_tag="PKN92_03650"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HNZ47919.1"
                     /translation="MVKFTGILVFMCIALANCCEAKELDAEEHKRPDVYTAHHSPDDG
                     EVPIVYVWVDEIFKEIPDDSVARYDALKLVASLQGLVNRDAPRMLIRFLEGEGQGGRI
                     NLDDYWLQITKEQWKVLQHTENVRSLRVVLQRFKEFYNGFVLWDPAVPATANVAATIC
                     GVEKLLPLRADSPLLPFLQESGFETNKGRNLCGLFTGAESGSAKCDAYLWAKREYLDK
                     GKCNPALMAFYIDAYSQQPGKPGFHYRDLPNSMVTNHDYFIANRAFFFDLLAWKDELP
                     VDDPAQSPGTDYETLVALLNSQYHQNHGKCFTSIGGFVSWDLKYTNHGAAGGLHEPVP
                     TEWEYVSLFSRYNAFLDADALGLACLTNASAYRHYPLKKKYSQQPRPALPPLENKHYV
                     LIYMGDYDSAAWLSRHVPLFWDDPNRGTLPIAWAFNPNLSDRVPHVFDHFYGTASPQD
                     WFIAGDSGAGYLNPNLLIGDRAGSGLPEALELWVSHNEKYYRRFDYSITGFVINGFHG
                     EMPLVIQDAYSRFSPEGVGMQLGMEKSLVNNTPFLRHSSDIYPDRNHPEKAADQMIAF
                     GKAETPQFLIFRWILQSPSTMKKVIAAVQERHKGDDWVFCDPYTFFGLYRQYLKKNKT
                     GFMFRSWSEFFDKLSCNCSAKHIR"
     gene            complement(19592..20125)
                     /gene="lspA"
                     /locus_tag="PKN92_03655"
     CDS             complement(19592..20125)
                     /gene="lspA"
                     /locus_tag="PKN92_03655"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNZ47920.1"
                     /translation="MTIKRKIIIIIAIVATVLLLDHGTKWHAIRTLKHSGNCYSFLGD
                     MIRIQYAENKGAFLSLGAALPTATRSLIMVGVNALILGVLVAYLLLAKLIKPLPFVAF
                     ATITGGGIGNLIDRIFRDGSVVDFMNMGVQIGGYSLRTGIFNVADLAIMAGLFLVIGH
                     ELYWGIKQQSHAEGGKS"
     gene            complement(20422..21300)
                     /locus_tag="PKN92_03660"
     CDS             complement(20422..21300)
                     /locus_tag="PKN92_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ47921.1"
                     /translation="MSLSPLLKIKTLALLFPLLCLYGCIKPQPGDSGANTNVVAAAQE
                     TKVAEGISSGTSLMEQWTAVATMDPKTMDLHKGFMLAQQMAAPGGEGLEGIFAVLADP
                     EQPASAKMLAVVSLGTHVKKEDFNRLKELTAPDKDIATRGCAAHLIGSIETPEAFDLL
                     KQLSQDEALHVRKIAILVLMRKGDGKAVEDAAKLWFLPEATDADRTEIIYGFPEIFAA
                     DHLDLFADALARTGLEEAVHYHALQILKTHGTAAQCESIQKFIDWTKNPNMESFARTV
                     LADIQQRVGASEKGES"
     gene            21468..22319
                     /locus_tag="PKN92_03665"
     CDS             21468..22319
                     /locus_tag="PKN92_03665"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNZ47922.1"
                     /translation="MDDYQKQFNQIIDEVRLLAKKHARGRPPHILLSKETAEKLLQID
                     ALKMRTEKEACAANMPPEQESCNKAQDSAGMPAVSGKDTDALAISVSSQAALEAIAEE
                     ILICKKCHLSEKRTKAVPGEGSVNASLVFVGEAPGASEDAQGRPFVGRSGQLLTDIIE
                     KGMQLTRQDVFICNVVKCRPPDNRVPSPDEVRICEPYLLRQLEILKPKVICALGSVAA
                     QTLLKTTDPIFKLRGLWHDYHGIACRVTYHPAYLLRNPPDKKKAWEDIQEIMRFMREN
                     EIPLSPQ"
     gene            22337..23710
                     /locus_tag="PKN92_03670"
     CDS             22337..23710
                     /locus_tag="PKN92_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ47923.1"
                     /translation="MTNASFVAPPLRWRRSLFFRVVLLCAVLLICLLASVIVITHHFF
                     QEIMRQMETQAMEIAHSLEIQIEEGFDGDYEALGSKLMDLYHGVDIKLDEQTGDLGAA
                     TFTLQRLDDGSIVRMARVPIVNNDQPMVMTVIMNIAPKTEILRAFRNRNLVILIIVFL
                     VTLGFMVYFIGKALRPLAVLSESCAAVTEGHLRPVNTQGAVGEVLLLENTFNRMITSL
                     HEKELMEAKLRQAQRLSALGNLAAGIAHDVRNPLNAIKLLSSHAAALLENDNHPAEKS
                     LKTVSREVDRLEEIVSSFLSLARETEMHREFVEIDGLVAECVRLFQKEAESRSVRLTH
                     DLRCSGLNLYLDAKQCTRAVLNVLMNAIDACPPDGRVRVFTRRTTTHCEIEIRDDGPG
                     IPKEVMDTVFDPYFTTKPGGTGLGLSITRSIVEEHGGTIQLSASSDRGCQVLIVFPLD
                     KAAGVVQ"
     gene            23716..25101
                     /locus_tag="PKN92_03675"
     CDS             23716..25101
                     /locus_tag="PKN92_03675"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ47924.1"
                     /translation="MQKQRILVTEDDQVQREIIADILEYAGYEVRPCSSGAEALKALQ
                     EENFHVLLTDMKMPGMDGLELLQQAKRQCPDLDTVVMTAHATIKTAVTAIKEGAADYL
                     EKPFGKDELLLVMDRTLERGRLRRENALLSRLVDDSVSLGNIIGQSHAMREVYERTRR
                     AVQVPSTVLIQGESGTGKELIARHIHFTGPRAKKPFIVVNCAAIPDMLVESELFGHEK
                     GSFTGADSMRTGKFELADGGTLFLDEIGDMRLESQAKLLRVLQDGLIEKVGGSKSRKV
                     DVRVIVATNQDLKRCVAEGTFRQDLYYRLEVLSITMPPLRERMEDLPLLINHFKNKIG
                     RKLNISVPEVGPDVLEAFRCYRWPGNVRELENTLEEAFILARSPLLRKQDLASKFQSP
                     EPEVSDIKLPPGGLDLEELEQELIRQALERSGGSIKAAAPVLGMTYKTLQYRLKKYNI
                     DPKGDAEDTGE"
     gene            complement(25178..25753)
                     /locus_tag="PKN92_03680"
     CDS             complement(25178..25753)
                     /locus_tag="PKN92_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47925.1"
                     /translation="MMTPEISAQEPGCLQYEGMIAQPDMSYFYLGFIVDKASMKTISP
                     SDVFQLTVGSKTLEVSNQVASAYFKITAVGDDWKPKPEALDSDLKGPFAYPNSGNGTS
                     DTIYRLREGVERFMITDINNPAASNKAQSEILIMSDLLSNTGADQLFNHIPGGCNVLF
                     MDGHVAFQKYEAQGDIPCNALIANAIGLLAQ"
     gene            25804..26490
                     /locus_tag="PKN92_03685"
     CDS             25804..26490
                     /locus_tag="PKN92_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HNZ47926.1"
                     /translation="MVTVNDERENQWIRHTFGVLDKSIYIGANDIDEEGRWVWQENGT
                     QFWSGVADGSAVNGRYSNWNSNEPNNYNDHEDCGMMYGSSGKWNDLSGTALNPVLLEK
                     NAGVVIVEGPFPANATIRAGKKFQFQVDVLQSWGTISYQWTHNGVDIPGATSDVLNFD
                     PVVEEDAGLYACRLTDGTGAHAETGTAALSVTPGGVMPLLHSASLLLLVALLMSIVGA
                     GALYRRNAAL"
     gene            complement(26513..>28453)
                     /locus_tag="PKN92_03690"
     CDS             complement(26513..>28453)
                     /locus_tag="PKN92_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNZ47927.1"
                     /translation="KAQLEDKPIFLSIGYAACHWCHVMAHESFDNPEIASLMNRFFVN
                     IKVDREERPDLDDLYMSAVVALTGRGGWPASIFLTPDLEPFYAGTYFPPTSRYGIPGF
                     SQILEEIARLWRSDRSRIQTQAAALTDRLKHHFTVVPASHMVEPETLVDQALAVLAAT
                     FDSQWGGWGDAPKFPSHAAVALLLRNGKRREIPHYLHMAEKTLECMAAGGIHDHLGGG
                     FHRYSVDAQWQVPHFEKMLYDNAHLVLSYLEAWQSTAKEKHRHITESILNYVLRDLTS
                     PEGAFYSSEDADSEGLEGKFYLWSYEELQKGLSAEHLAFLKKHFSVSEKGNFESHEPG
                     HEGLNILSAKAWIIGEPWDTIRKILLEKRSRRIRPGLDTKVIASWNGLMITALAKAAF
                     YLKNDVYRDAGEKAGHFVETHLLSDEGLFRIWSDGKASHPAYLEDYATVTNAFLDLYQ
                     CTFNRRWLALAKGTGERMIHLFLDKKNGGFFTTGEQHKHLFARMKTVHDAAEVSAAST
                     AALGLMKLAWHCDEPAFENAALTVWQTGAPSAQNAVQGHLHLALFADTLQHGFTEATL
                     RPGCTAADLEALLEVLRQEAPLNTLLKLERDPESSDDATAPPQTNQSTHSAVYLCLHR
                     SCLPPISSAKELRRRLRNASAD"
BASE COUNT         6929 a         6855 c         7344 g         7325 t
ORIGIN      
        1 tgggtgcagc agaagccggt gctcgagaac ggggcgtgcc agagatttga catcatcagg
       61 cagcacgtag tttcgtccct gaattgcggc gaaagcctgg catgcccgga aaagcatcat
      121 ggaggctcgg ggactggcgc ccaggcttaa atgggtgcag tcgcgggtcc ggttaatgag
      181 tttaagcata taatgtcgta ctttttcatg gaagtgcact tctttaatag cagcctgcat
      241 ttttaaaagt gtttttggat cacacacttt tttgacagcg tctatcggat gggtaagcct
      301 cgtgttttca aggagttttg cttccgccag caagccggga tagccaagag aaaggcgcat
      361 catgaaacgg tcaagctgtg cttcaggcag cgggaaagtg ccttcatgct ctacagggtt
      421 ttgcgtggcg aatacaataa acggctgctc cagctgatat gtgtgcccgt cgatggttac
      481 ttgtttttcc gccattgctt ccagcagagc ggattgggtg cgcggtgttg cccggttgat
      541 ttcatcggcg accagaatct gtgagaaaac gggaccttcc cggaaggaaa attcctgtgt
      601 ttttgggttg taaatggaaa cgccggtaat gtctgaaggc agcagatcgg gtgtgcactg
      661 gatacgattg aaagcgcagc cgcttgctac tgcaagggtt ctggctagca ttgtttttgc
      721 cacaccggga acatcttcca gaaggacatg cccgcctgaa agaacagcgg tgagtgcgag
      781 tttgattaca ggacctttat ccagaatcac ttcatccagt gcgttcgtca gtgcgttaac
      841 ctgctctctg aaattattca tactgtctcc tgattcaaag gaattaccat cccctgctct
      901 tcattgctgc aagcggttcc cggtcggttg catgaggcgg ctccggcgag agagcagaaa
      961 gggcggctct ttcttccggg tctaatacaa tatccagaca gcccagggtg tcctgcagct
     1021 gctctattgt tctggcgccg atgatgggcg ctgaaacgga aggatgcgat ttgacccagg
     1081 cagcggcaag cgccgccggt gaatatccct tttgtgatgc ataggcgata aaacgcctgg
     1141 ttacatcttc atattgaggg tttgcatagc gctgccggta catttccgct tctttaagac
     1201 ggcctgatcc gcctgttaaa tatttgccgg taagtaaacc ggcaccgatg acgttgtacg
     1261 gaaaaaccgc aagtctttca tactccgcca aaggaaggag ttccacttcc gcctgccttt
     1321 tcacaaggct gtacatcggc tgtatgcagc tcacaggagc aaaggacttg atccgggtga
     1381 tgcccatcgc tgttgccacc tgccaggcgg aaaaattaga aatgccaagg taatgtactt
     1441 tcccctgctc tacaagtgta ttcactgcgg ccagggaaga ctccaggctg cagttttcat
     1501 cccaatgatg caggtagaga atatcaacat aatccgtctg gagacggcgg agtgatcgtt
     1561 caagagacgc gaggatatta cggcgggaaa gcccctgatc gtttctgccg ccgccataag
     1621 gaaaaaatac tttcgtggcg agaataaact cgtcccgcct gcccttcagc cagcgcccga
     1681 cgatttcctc cgtcagtccc ttattataat tatgggcaca gtcaaaaaag ttgatcccca
     1741 gcgaagcggc ataatccatg atggaaaagg ataccgcttc atcggcttca ttgccgaagg
     1801 tcatggtgcc cagacatatt tcagaaatct ccagtcccgt gctgccgaac tgaacgtact
     1861 tcacgctctt atgctccctt cttgtcttta caaaggatgt tcatgttatt gtatagcctt
     1921 tatggataga ggttccaatt acgagcggga aatgaaacag caatcaaaag caaatacgga
     1981 gaaaaaagca caagtttttt ctgcactgta cggggattgt caggaacgtg cttttgagca
     2041 gacagtactg caggaaacgc ctgaaagtga tttgcaggca ctttggcagg gactgattct
     2101 tccgactact cagaatacgg ttgaaggaca tcctttccga atcattgctc cggggtggtg
     2161 gaacaaactg aagggccccg atttcataaa tgcccagatt gaatttaacg gaaaactgat
     2221 cacgggcgat gtggagattc atcgtcacaa ccgtgactgg tttcatcacg gacatcatga
     2281 ggaccccgca tacaataatg ttgttttaca tgccgtggaa acagagggag cccctgcggc
     2341 ggtgacccag tcaggtcgca ctgttccgac ctgggttttt ccgccgactt cacagagcga
     2401 tctggatatg aagcgccaga taagcagaca gcagtgcggt gaatgcgcta aatcttatgc
     2461 atttcgcaat ccagctctgc ttgagcattt tctggatatt gccggtgact ggcggattcg
     2521 ggaaaaagaa cggcgtatac gaaaacgcgc cgaagaagtc gggtttgacc agaccgtcta
     2581 cgagagagtg ctggaatctt gcggctacag tgcttttaaa aaagagtttg aactccttgg
     2641 aaaagcgctg ccttacgaac gggcactgca gttggggcgt gaggacaaga tggcgcttga
     2701 agccgcttat ttaaggctgg gcggctttct tcctgtggaa tggatggtgc cggatacaaa
     2761 ggcgcccgct cattttaccc ggctggtgca ggcctatgaa cagtaccttc atcccatgga
     2821 aagcctggga atcagctgga aaaagcaggc agtccgacct gccaacagac cggagagaag
     2881 gcttgcaggg atggcaggct tcatgtgcaa agtcaccgac cgggggcttg aaaggcagct
     2941 ggattttttg tggagacagc ctgtttctgt ccgggagcgt atagataatt tctgtgccct
     3001 cttcggaaag aatctaagct actgggcaaa ccattacacc tggggaggaa aagaactggg
     3061 gaaagcggct gccgtactgg gagaaggaag agcgcgctcc atcctgggca acatttttgt
     3121 tccggcaggt ttatttctgg cacgccagga cgcagctgga tccctgcttg aaaataacgt
     3181 tcatgaattg ttcagaacaa tgaaggctga gcccaaaaac aacgtgtatg aatccatgct
     3241 aaagtggatc gtaccgcccg gtgtcaaaat taaaatgaat ttcagacgac agcaaggact
     3301 tctgcagctg agtgcggact ggtgcgagcg caatccttcg tgtcacaact gtattcttcc
     3361 tgttttcctg caggtaatag acgaaggcgc ataccagtct gtatttgatt caggtctctt
     3421 gtaacaaggt gtgccttcag gatgcagacc tatttcagaa tctttccttt ttaggggagt
     3481 agcgcttttc ccggacgacg gtacgcgctg gatgattttc cgaaaataac ttactttgca
     3541 tttaggtgtc ccaactctta tgctttatgc atatacggaa tcgttccttc tatgaaagac
     3601 cgttatgcgc ctgaataacc tccaaaaaca gagctccatc ggggggatgc taattctgct
     3661 cttcctcact tccggatgct tttcaaacgt cacccgggaa gccggtgtac tgcaggttgg
     3721 caacggggcg gaagtacagg agctggatcc tcatattgtg acaggtgtga cggaacaccg
     3781 tgttctcact tcgcttttcg aaggacttac agactgcgat tcatccactc tggagccggt
     3841 gcccggcgtg gcagaaaact gggaagtgag cgaggacggg ctgcattatc gctttactct
     3901 tcgcaaggat gcccgatggt ctaatggtga cccggtaacg gcagaggatt tttcttatgc
     3961 ctggcagcgt attctatccc ctgctttggc ttcagaatat gcctacatgc tttattgttt
     4021 gaaaaacgcc cgggcattca atgaaggaac aatcactgat tttgctgagg taggggtttg
     4081 tgctgttgat tcctggactc tggatgtttt tctcgacaac cctactcctt acttcttatc
     4141 catgcaaaac cactatgcct ggtttccagt gcatcgcaaa acgatagaat ctcacggaag
     4201 catggatgaa cgtggcacac gatggacgca acccggacat cttgtcagca atggaccttt
     4261 tcttcttcag gcgtggctgc ccaacgaaat actgagcgtc cggaaaaatc ccttttactg
     4321 ggataaggag catgtgcagc tttccgggat tgattatctg cctatagaca atctgcaaac
     4381 agaagaacgg gctttcagat ccgggttaat tcaactgacc tcaaccattc cgctgcagcg
     4441 tgttgagagc taccggaaaa aacacccgga actgctttgc ctgcttccct actatggcag
     4501 ctatttttac aggttgaatg tgacccgccc tccttttgac aatgcagacg tgcgaaaagc
     4561 ctttgctctg tctcttgatc gtcaggaaat cgttgataat gtgcttaaga gcggtgaaac
     4621 cgctgcgttc agttatgttc ctgaaggcgc tggcgattat acctgcaccc atccgctgcg
     4681 ctttgatgtc gccaaggcgc gcgcgctgct tgcgtctgcc gggtatccgt ccggccgggg
     4741 ttttcctcct gtggagatcc tctacaatac cagtgaagcg catcgaatta ttgcggagac
     4801 cgttcagcgc atgtggcatg acaatctggg cgtggaagtt cggttgttaa atcaggattg
     4861 gaaggtctat ctctcagcac tcaatacgct ggactactcg atttcccgct cttcctgggt
     4921 tggagatgtg gcagatccca ttaattttct cgaatgcttt cagacagatg tgggaaataa
     4981 cagaacgggc tggtcgtcac agcattatga tacgctcatt aaggaagcgc atcagtgtcc
     5041 tgataaggaa aaacgcattg ccttactgca ggcagcggaa acagtgcttt tggatgaatg
     5101 tcctattgtg cccgtttatt tttacacctg gaaattcttg aaagctcctg aagtgaaggg
     5161 gcttaccccc aatatattgg gttacattcg ctggaaagac ctgtcactcg atacggcgga
     5221 agggtaaaaa atggccgcct ttattacaaa acgcctgctt gaatttattc ctgtactgct
     5281 ggctattatt accattactt tttttcttat aagacttgcg ccgggcggtc cttttgatac
     5341 ggaaaaacgg gtgtctccgg aggtgctggc gcaactggaa gcgcattacc agatggatcg
     5401 accgctgcac gcacaatact ttcagtattt gaaagggctg atacgtctcg acttcggacc
     5461 gtcttacaaa aaaccggcgc ggacagtggc gcaatggctg ctgctccgct tgcctgtgtc
     5521 tattgagctg ggactctacg ggctggcctt tgccctgttt ttgggtctgg gtgcaggcgt
     5581 actggcggca ctgaagccga attcctggct ggaccatacc gttatgtcgg tcgccatgct
     5641 gggcatttgt gttcctgcct ttgttctggg accgcttctt gtcctggtct ttgctctgtg
     5701 gacagactgg ctcccggtga tgggatggat ttctcccgcc cataagattc tgccttccat
     5761 cacactggga gcaggctatg cagcgtacat tgcacggatg gcgcggggcg gtatgctgga
     5821 aacactggca caggatttta ttcgaacagc acgggcaaag ggcatgaaag aaagccgtgt
     5881 tgtcttgaaa cacgggctgc gtgccggtct tcagccggtt gtttcatttc tcgggcctgc
     5941 tgttgcgggt ttgctgacag gctcttttgt cgtggaaacc atttttatga tacccggtct
     6001 gggcagagag tttgtggagt ccgcttttaa cagggattac accatgataa gcggcgccgt
     6061 actggtttac gctgtgcttg tcatgagcct gaatctggtt tccgacatac tgctgggact
     6121 gctggatccg cgcattcggg gacaatgatg actgatcgcc gcagcaatgc tgttcagaac
     6181 aattccagat tttccggagg gggcggcggc tcaaaatgcc tgtggagccg gatcataact
     6241 tccgcatcgg ctgccgcata gtctatttgc tcaggcataa gcggtcgacg tgtccaatcc
     6301 gatgtctgat agtgtttgtc cagatagata ttgagagcgc gaacacacac atcatcgagg
     6361 cggtaactgc cgacgtcagt ttttttgtag tgctttcttg catgcaccag tgtatcgtaa
     6421 atgttgtgga tggcaatgcc cttctctccg aggatctgct gttcaaaatc cttgttatga
     6481 ataattttta ggatttcttc gttttccatc agatgcttca gcggctcaag atcggtgatc
     6541 ttgagagcat caaaaacata aacctcatgt gggctggcaa gctgtaccgt acaaagagtt
     6601 cgtggctctt caagggtcgt ttccacatca agtcccagca gcgtttcttt atgcagagcc
     6661 gtgcaaattg cattgaattc ctcctgaata tccacatagc ataccggcag ctgcgatttg
     6721 tatacaccgc ttttctggat taagtggagg acgtgcccgt gcgttacaga caccttggac
     6781 ggcttgatct gcagaacagg cagtccttta agcacgatgc ccacttcttc tgtaattttg
     6841 tctgccggta cagccccggc cttttcgctg aaactggaga caagaagcat gcggccgccg
     6901 gggcggacat aattaaaccc gtctctaagt atttctttat tttgctctga aagggctccc
     6961 gcatctctga tctgatcaat gagcaccaga tcatagtacc ggcggacatt ctgcaaagta
     7021 ctccccgctt tcttttccgg ttccggccct tcgacattat agcgtcgggt gcgcagccag
     7081 gaaagcgcag tatcctctgc aggacggtaa caaaggatcc gttgtttttt gctgaaaagc
     7141 agtgtcgggc gcaagcgcac cagaatggaa gaattcatta gtataaaaca cttgttctat
     7201 tgtcagtagt aattaaaaca gatgtatata gtgtcgtaat gaattgaaca gatttcagac
     7261 cggatcacct aaagccagcg catcgcggcg catgcgctca gcatcgtttt tatccagcgc
     7321 aacattgccg gcaccggcgt tgtccagtgc ctgcgcaaca gtgcgcatgc cgcacaggac
     7381 atgggtaact ccgggctgaa ccagtgtcca ggctatcacc aattgtgcaa ggctgcattc
     7441 gtatttttca gtcaaatccg accatccggc gagcatatcc aggactttag gccggttctc
     7501 agctttaaac cagggattcc agtccgtatt ggttcgaaat tcgccttctt taaacgagcg
     7561 gctcagaccg actttgccgg tgaggaggcc ttgttccaga gacatatatg tgagggtagc
     7621 aagattgttt ttctgacaga aaggaagaat atcgctttcc ggatcccggt agagcatgct
     7681 gtagcggaat tgatcggaag cgatatcacc gcatgcgcaa tagcattcga gctgttcaac
     7741 agatacattc gaaacgccga tagatctgat ttttccttct tcctgcagct tcagcaggca
     7801 tcccattgtt tccggaatgg gtgtgttttc cggatctttg gcgggccagt gaacctgata
     7861 caaatcgata taatcggttt gcaggcgctg gaggctgttt tctatttcct ccgcaatggt
     7921 gtccggacgt aaactgatga aggtgtcctt tccattcaga tggtaatgaa atgagccacg
     7981 gttatcctgc caccagatac cgcacttggt cgccaaaaca accttatccc gtttgccttt
     8041 aatggcctgt cccacaattt cctcggcatg cccccagccg taagaagcgg ctgtatcaat
     8101 aaaattaatg ccctgatcca aagcggcgtc gatggagcgg atcgattctg catcgtctac
     8161 gccgccccaa ctttcgccgc cgcctgtcac ccaggtgccg atggcgatca cagatgcttc
     8221 aataccggat tttcccagtg ctttgtattg catagttcct tttcctttgt atttttggag
     8281 aattgctctg ctggtttgaa atcggagtaa tgataccacc ctttttctgt aaaaccaaag
     8341 catgtttttc tgaaatctct cttcgcacag tgaaaacagc agaaagttgc attttatatc
     8401 tgcatttgct acaatctggc ttccatctga aaactatccc tccagagtgc tgtgcaggca
     8461 cggtactttt tattttttac aaggagacaa tcatggcaag agtatgtcaa tacagcggaa
     8521 aacgccctca agtcggaaac cgtgtcgttc gtcgcggtaa agcaaaacgc gaaggcggta
     8581 tcggacagaa tgtaaccggg ctttccaagc gtcgctggaa tcccaatctg cagacgatcc
     8641 gtgtgcttga tgaaaacggc accgtccgtc gcgttaaagt ctgtgccaag tatatcaagg
     8701 ccggaaagtt cgtgaaagcc cctcgtggta cccatgctgc atggaaagcc gctgaaggaa
     8761 aataatttta actgctgatt acaatgcccc ggaccggcgc caacccggct cggggcattt
     8821 tttttcagcc tccgggaacc aaagaaatgg cacaattaac cgatttaacg acggatagcc
     8881 ttgtagaatt acagattgac agtgtcgccc acggcggaga tggagtcggt cgcctgtcgg
     8941 gtctcgtgtt ctttgtcact ggtgcttttc cgggtgatcg ggtacgggca cgggtgaccc
     9001 ataaaggcag gcgtgctgtc tggtgctctc ttgaggaaat catggagagc ggcccctgcc
     9061 gcgcacttac aggtatttcg gacacgctgg cgtgtgcttc ctcatgcttg tggcatgact
     9121 ttctgtaccc tgctcaggcg gaatggaagt gcagaatcgt ggaggataat ctgaaacgaa
     9181 tcggcgggct gagcgctgag ataaagtggc tggaagaccc gcaattacgt acaggttaca
     9241 ggacgcgtgc tgtttttcat ggcgatggca aagtgctggg ctgttacgcc cgccgcacta
     9301 gagaaattaa aacagcaacg ccctgccctt ccaatcacca gcgactgctt catgcctttc
     9361 aatcacttca atctgcgggt atttatggag atgtccagat aactgtcaat cctgaaggtg
     9421 atgaagtact ggtgcatctg caaaaagact ctccggaggc cagacttcgt tttccgctgg
     9481 tgaataccgg agatgacaaa aaacggcacc actttgtttt tgacggcgtc cctgtagtga
     9541 acaattcttt ttcacaagca agtctgctcc tgaaccgtat actcagaaaa aaagtggata
     9601 cctgtatcgc tggggcgggc agtctgctgg atttatactg cggcagcggg aatttcagtt
     9661 tgcattattg cgactctgca aaagttctgg gcgtggattc caatgtgcat gcgataaaag
     9721 cagcgcagaa acatgcgccg gggagttatg agtgtggcac ggaaacgctt atgaaaaagc
     9781 tgattcagga atcggtatgg gacgttattc ttctggaccc tccgcgaacc ggtgcacagc
     9841 agctcatcca ctgtctgaaa gaagcgcggg caaagcggat tgtctatatt tcatgcgatc
     9901 cggcgacact ggcccgtgat ttgaaaatac tgtcttcatc aatgagatgg cacctgaaag
     9961 aagtgactgc aatcgacatg ttccccggta cgcctcacgt ggaaaccgtg acgcttcttg
    10021 aaacagacta aaccgggcac agtccggaaa agatcaggaa gataaaagtg actattagca
    10081 agatttgtgt atatgcggcg agcagtaatg taatcgcacc tgtgtacaaa gcggcagcgg
    10141 aagagctagg tgttttattt gccgaacatg ggaaaacgct ggtctatggc ggtggcaatg
    10201 tagggcttat gggtgtgtgt gcccgggcag tccacacccg tggcggtaaa gttattggcg
    10261 taattacaga caagctggtc gatctcgaat tggtttatga aaaggcagat gaactggtta
    10321 ttaccacgac catgagtgag cggaagcgga ttatggcaga ccgcgccgat gcttttgttg
    10381 ctctccccgg aggtctgggc actctggatg aaataattga gatactggtg ctgaaacaac
    10441 tctggtatca tcataaagcc tgtgtgtttt taaacaccaa cggcatttat gattctctct
    10501 ttcgttttct tgatgacttg accagagacg gtttttcacc tgcttcccac cgcagcttct
    10561 accatgtttg cagcacgcct gcggaggtca tgtcctatct tgaggcgttt gaaccggaac
    10621 ctgcacacga aaaatggttt tgattttcac caccatttgt tgccagcccc ggtttgaagg
    10681 tgatggcagc atcttgccat acttagagag ggcgcaggtc ttcatttttc agagaacggt
    10741 ttgagcgaca acaagaatta gtcccgggag aagatgattg gcaacctcgg attctaaaga
    10801 caaaaaggaa ttgtcctttt ttattctttt tctagtgctg ggtctgtttt tcctgtcggg
    10861 tgcctgctcc cttgtgtatc aggtcgtatg gacccggaag ctggcactgc ttttcggtgt
    10921 cacctcccat gctgtaagca ccgtcctgag tgtttttttt ctggggcttg gtgcaggcag
    10981 ttttgctggc ggacggattg ccgaccgaag caggcatccg ctgcggcttt acggtctttt
    11041 tgaatgcatt gtcggggtga gcgctgtcct ggtgcttttg ctgctctctg tcagtgagtc
    11101 ctggatcccg actgcactcc ggctctttga tttttccaga accacaggcg ttcttttacg
    11161 cgcaatactg agctttcttc ttctctttat tcctgtaagt ctcatgggcg caaccctgcc
    11221 tctcttaaca cgcttcgctg tgcgcgctca ggagaaaacc gggtttcacg tcagtattct
    11281 ctatgcagta aatactttcg gcgcagttgc cggctgcgcc acagcaggct tcgtgctgat
    11341 tgcgtctctg ggattttcaa aaactgtata tcttgccgct gcccttaata taggcgccgg
    11401 actggctgcc gttctgctct ctctgttgaa ggagccttta acgcaatcgg cggttcccga
    11461 aaacgataat gctacaccgg ggtgtggcag ccggggtggt gccggtccga tgcggctgct
    11521 gatatttgca acggcacttt gcggcttttg cgggcttgcc gctgaagtag tgtacacgcg
    11581 gctcctttcc attgtttttc tgggaaccac ctacgccttc accactatgc tgacagcatt
    11641 tctttgcggt attggcgctg gtggcggttg cgccgctctg atccagcgga aaatgcgcaa
    11701 agaaaatgcg ctgcttcttg tggggcttat ttcgggcggt atagctgttg ccatgctctg
    11761 gagtcttccg tggattgctg gacttccgga ccgttttctt gaccttcagc gcagcaccgg
    11821 gcttgtgtgg gatgccacac tgcgcggcac cttttttctt gcctttctaa cattatttcc
    11881 catgactttt ctttttggag tggcatttcc tctggtcatc ggggcgggcg gattatggcg
    11941 aaaatcagtg ggcagtctgg tgggactgat gaacggcgcc aatacgctgg gcggtgttgc
    12001 cggtgcgatg gcgggcggct tttttcttct gcctttgctg gggagccaga acagcataac
    12061 cttcctcgca gttcttttgc tggtaaatgg tcttgccttg attgtactgg gcagaacatc
    12121 gggtaaaagt atccgggtct caatcgcttc aatacttctt ctttgttttt ttgttacatt
    12181 cctttttttg cctgcggacg tgaatctggc tttaaataaa ggatatatgc cccgggatca
    12241 tgaagtgctt tatatgaagg aaggcacaga aggaaccgtt gccgtgagcg gtcccgttgg
    12301 tgcacagcat ggcgaagacc gggttttatg gattaaccgg gtgcaggcga ccacttccat
    12361 tgaacggggc gttaaaatga accgtttgca gggcgttttg ccgcttcttt ttgatcgaac
    12421 ctgcgagcgg gttctcttca tgtgctttgg atccggcatt acctgcggta cgctcgcact
    12481 ctcgcccttt gaacagatcg atgcggtaga gattagtccg gaagtactcg cagtagctcc
    12541 atactttgat aaagacaatc tgggtgtcct ctacaatccg gctgtttctt ttcatattga
    12601 tgacggcaga aattatctgc tcagaacaga ccggaaatat gacctcatta cctttgaacc
    12661 catgccactg gcgctggcag gtgtctccac tttttacaca gaagagtact atcgtttgtg
    12721 ccgaaaatgt cttgcttctg gcggcatcgt atctcaatgg atccctttgc acagcaatga
    12781 ccctgaaatg gtgagatcgc tggtgtttac ttttatctcc gcttatcctg aatattgtgc
    12841 gttttttgtc aatgcggatc tttttctgct tgggtcggac atgccgttgc gcctgaatta
    12901 tgccaaggca aaggaacggc tgaaaaattc tgttcttgca gatgcgctgg aaaaatccgg
    12961 gcttggtgat ctcgctgaaa ttatgtcctg ctatctcttt gataaacagg cactggatgc
    13021 ttacgcatcc gggggtgtca gtatgagcga tgatctgccc tgggcggagt ttaccgcgcc
    13081 ccggcttgtc tatgcccgtc ttgtgcctga agcggtagat actcttttgc cttttatcag
    13141 caatccgctg gaagcagtga ttcccgggag tattactgct gaagaacgcc agcgcctgga
    13201 aagacgctgc cactcccgcc gccttgattt tcctgcattg aaagaatatt acggaggcag
    13261 catgtttgat gacagggcgg ccgatggttt cgctgcctct ctcgaagctg atccggaaaa
    13321 tctgaatgcg cgctattacc tgaaaacgat tgtgctggca cagggagagc gttttcttca
    13381 gtgggatgac tgggagcggg ttatagccct tacggggaaa gcacttcgct tcatgccgga
    13441 tgataaggat ttgactgaac tttatcaggc ggcggagcaa aagagagact tgtctgcggg
    13501 cagataaaaa ggaacagcgc tttggcaccg aaaaagctcg gagaacactc atgaagtctt
    13561 tctggaaaag agggcgactg cgggcggcag tagcctgtgt acttcttatt gtcttttccg
    13621 gggttctgct gaaaaagacc ggcagagatg agaacagagc gcccgatttt gcacctgcca
    13681 ctcccccgtt ttcgtcacaa tcttatattc agtccggcag ctgtgccagt ctgccaacag
    13741 agagcataca ggtttttttt acgacgcccg ggattgaagc cgccccgcct atccttcacg
    13801 cttttctgca gctgattgat tccgcttccg tacagatttg ctgtgctttt tatgacctgg
    13861 aaaatgagga tgttgcccgg gcactgataa acaagcatca tgcaggggta caggttgcca
    13921 ttgtttccga ctccaattat gaccgcagaa aagcagtaaa gcaatgcctc cgtgcaggaa
    13981 tacctgtggt ttttgataca agcaaagcct ttatgcacaa caaattctgt gtagttgacg
    14041 gccgcttcgt ctggaccggt tcaacaaata taacgcaaaa cggcttttat aaaaataaca
    14101 ataacgccct gctgatcaat tctcaggagt tggcagtcaa ttatattaat gagtttgaag
    14161 agatgttcct tcaccggctt tttggtgcag gatctcccaa aaacacagcc tttccccttc
    14221 tgtccgtttc gggtatttcc atagagtgct attttgcacc ggaagacggc gcagcacaaa
    14281 gaatcatcga ttgcattcag actgcaaaga ccagcattga ctttatggcc ttttctttca
    14341 cttccgtgcc cattgctgaa gcaatggctc aaaggatgcg tcatggcgta aaagtccgcg
    14401 gattgtttga aaagcgcgat gcggacagcc agcatagccg ttaccggttt ttaaaagaga
    14461 atggcgctga ggtacatctg gatatcaacc cgaataatat gcatcacaag gtcattgtgg
    14521 tggatcggga gcatgtaatt acgggcagct acaatttttc taaaagtgcc gaaaccagaa
    14581 atgatgaaaa cattctgatc ctccacagcc gggatattgc ggattgttat gtgcaggagc
    14641 tggaaagaca ggtgtcctaa cggaagctgc tttactcttc aaatttctgt tgcaggccgg
    14701 gcgaccatgt cgtaaagttc cgtccgcata aggcggacat ctataaaact tccctgtttc
    14761 agcggtgttt tgctttcaac aaaaatgact ccgtccactt ccggcgcttc cgatgcactt
    14821 cgcgcaatcc agcagtttct ttcatcgtcg tatccctcaa taagcgcccg gacggtcttg
    14881 ttctctctgg acatgttaaa ggcttcagtg ataaatgcct gaagctccag cacttcattg
    14941 caccgtcttg taatggtatc ccggcggagc tgctttttca ttagtgtcgc ttcggttccc
    15001 tcttccctgc tgtaagggaa agcgcccagc cattcaaaag gcatgctttc catgaatctg
    15061 agcaggtgtc tgtggtcagt caggctttcc ccgggaaagc cgatcagcat ggttgtccga
    15121 agtgtaatgc ccggcacttt ctcccggatt tgacagatca gatcgccgat attcagggcg
    15181 tgggccggac gctgcattgc tttcagaact ttgggcgccg tgtgctgcag cggaatgtcc
    15241 aggtaaggaa cgattttcgg ctgtgcggta atttcttcca gaagagcctc tgtaatgcct
    15301 ccgggataac agtaaagaca gcggatccag aaatcgccgg gaatggcgca gagatcagcc
    15361 agtaaatgct gaagtctgta ctctgcgtag aggtcgcgcc cgtaggcagt gcagtcctgc
    15421 gctaccagat ttaattcacg tacacctgac tcaatgagga ctttggcttc gtccagaatc
    15481 tcttgggaag gtctggaatg aaggcgtcct ttaatggcgg ggataatgca gaagctgcag
    15541 gcatgatcgc acccgtcacc aatttttaaa aatgcatagg gcttgttgtc cagccgtttt
    15601 cttgatccgg aagagcaaga ggatacggcg cagcaagagc gtggtgcttt ttcaagcagc
    15661 agcgccttga gtttttgaag atcactgatc cctacccagg catcgacttc gggtatttcc
    15721 ttcttcaact cgtccgcata tcgttgtgac aggcatccgg cgacataaat ttgccggggg
    15781 aacccgaacg cttcacgatg gtttacccag cggaggatag cctccacgga ctcttctttc
    15841 gcatcatgaa tgaatgcgca ggtaaagagg acgacggcat ccagatgagg cagatcgtct
    15901 tcgggaaatc cagggcataa aacagttatt tccctgccgg catcgagatc cagaagaccg
    15961 gcaaagtgtt cggcatctgc cgtattcttg tcacatccca aaacagcaat gccgcattga
    16021 aaactcggta ctgtcatagc cgttacaatc ctgtctaatt aaaaaaggcg cagagtaaaa
    16081 aagaaaaagg ctttttactc tgcgccgggg aaaatcacga tgcgatcacg aaacgttggg
    16141 tacgaaccgc cacaacacgg tgccgataat cataagcgct acagaaacgc ccagtgcaat
    16201 tttgaggccc tgacgtatgg aagtttttgc cgtgtaattg ccgataatgg gcggaataaa
    16261 aataccgccg aacagtccga tggcaaaaat gagcccgaag acactgccgg ctgtgccctg
    16321 attgatggcg tctgttttgc cgaagcagac accgaccagt gtcgggaaaa cagggccgaa
    16381 agaaaatcct gccagaagcg ttccgaaaac acctgtcgca gggtttggcg cgatgatcat
    16441 gaggccgatg gcaagggcag cgatgagcgc cagcacaggc acaagaaccg ccggatccag
    16501 acctgtgaga aagatagcgg taatcaggcg cgccaccata agagcgatcc agaagccgct
    16561 tagaaaagag gtggcttttt catcggaaaa ttggtgcgat ttaaggtagg tggagacgaa
    16621 acctgccatg gaggattcca gtgctatata gcagaaaagc gcaagaccgc cgacaagcac
    16681 tacaggattg gagagcagtc ccagtgtttt cgcaacgttg aagcctgctt caggctgagg
    16741 caattgcttc atagtgagcg caacgaggat gatcggcaga aaaaggatag cgccgatcag
    16801 actgactgtc tttttgtacc ccatgttgct aagaagaagc gcaatgatca gaggcgtgat
    16861 aaacgcaccg atgccgaaga aaacattgag caggttggag gcgcggggtg cattctgccc
    16921 ttcaaaaaga actgtgggcc ccagtgtgtt tccgatggtg ttggcgcaca tggcggcaat
    16981 acccagtaca aggcaggcta tcagtgcagt agtgtaattg cgtgcggaag cgagcacaag
    17041 gacactgacg catgccagcc cgaaaccgat gagcgcaacc atgccataac cgactgtatc
    17101 ggtaagagga ccaacaatca gcaccgctat aaggcaggtg aacatcatgg cggaaatcag
    17161 gctgccgact ttggtgtcgt ctatgtttaa cgtttctgcc agtttgagtt tgacgcttcc
    17221 taaaatggaa aaaagaacac ccagtgtaaa aacgccggaa aaagcgacaa atgacggtcc
    17281 ggtaaacatt acatctcctt ccgttgcgcc ctctcaggtt ggaataaggg ctgttggtat
    17341 acgacattta ctcaaagcat cttttgcctt gaaatttggt aacgcttatc ttatatgttt
    17401 tgcgctgcag ttgcaactta gtttgtcgaa aaattccgac catgaacgga acatgaatcc
    17461 ggtcttattc tttttgaggt attgcctgta tagcccgaaa aaagtatagg gatcgcaaaa
    17521 gacccagtca tcgcccttat gccgttcttg tacagcggca ataacttttt tcatcgtgga
    17581 gggtgactgc agaatccagc gaaagatcag aaattgcggt gtttccgctt ttccgaaagc
    17641 gatcatctga tccgctgctt tttccggatg atttctgtcc gggtagatat cggagctgtg
    17701 tctcagaaag ggtgtattgt ttaccaggct tttttccatt cccaactgca tgccgacacc
    17761 ttccggggaa aaacggctgt acgcgtcctg tattaccaaa ggcatttctc catgaaaacc
    17821 gttaatcaca aagccggtaa tggagtagtc gaagcgtctg taatactttt cgttatgcga
    17881 aacccacaat tctaatgctt cgggaagacc gctgcctgca cggtcaccta taagaaggtt
    17941 agggttcaga taccctgctc ctgagtctcc ggcaataaac cagtcctgcg gtgatgccgt
    18001 gccgtaaaaa tggtcgaaca catgaggcac tctgtcggac aggttggggt tgaatgccca
    18061 cgctatgggc aaggtgcctc tgttcggatc atcccaaaat aagggcacat gccgggaaag
    18121 ccaggcggca gaatcataat ctcccatata aatcagcaca taatgcttgt tttcgagagg
    18181 gggcaaagcg ggccgtggtt gctggctgta tttctttttt agcggataat gcctgtaagc
    18241 ggacgcattc gtaagacacg ccagtcccag cgcatctgca tcaagaaagg cgttatatct
    18301 gctgaagagg gaaacatatt cccattccgt cgggacaggt tcatgcaaac caccggctgc
    18361 gccgtgatta gtatacttca gatcccacga tacaaaaccg ccaatggaag taaaacactt
    18421 accgtggttt tggtggtact ggctgttgag caaagcgacg agtgtttcat aatcggtacc
    18481 cggggactgt gctggatcat ccaccgggag ttcatccttc caagccagca gatcaaagaa
    18541 aaacgcacga ttagcaatga aataatcgtg atttgttacc atggagttgg gaagatccct
    18601 gtagtgaaat ccgggctttc caggctgctg tgaataagcg tcaatataaa aagccatcag
    18661 agcaggattg catttccctt tatccagata ctctcttttc gcccagagat aagcatcgca
    18721 ttttgcactg ccgctttccg ctcctgtaaa aagtccgcac agattcctgc ctttattcgt
    18781 ttcaaagccg gattcctgca gaaatggaag caaggggctg tcggcgcgca gtggcagaag
    18841 cttttctacg ccgcaaatag tagccgccac gttggcagta gcaggaacag ctggatccca
    18901 gagaacaaaa ccattgtaga attccttaaa gcgctgaagc accacacgta aagaacgaac
    18961 gttttctgta tgctgcagta ctttccactg ttcttttgta atttgcagcc agtagtcatc
    19021 cagatttata cggccaccct gcccttctcc ttcaagaaaa cgaatcagca tgcggggggc
    19081 atcacggttg acgagaccct gtagagatgc caccagcttc aacgcatcat aacgcgcaac
    19141 cgaatcgtcg ggaatctctt tgaaaatttc atcaacccat acatatacaa tcggaacctc
    19201 tccatcatca ggagaatggt gggcagtata cacatctggt cttttatgct cctcagcatc
    19261 cagttctttt gcttcacagc agttcgccaa ggcgatgcac ataaaaacca ggattcccgt
    19321 gaatttaacc attgttcctg cctgttttgg gtggagacca cgcgtttttg gtgttaaaag
    19381 tggatgaaaa cgcattattc atataattgt caactgcact ttaaaaggac acatgtcagc
    19441 ccttttgaga cagagaaaaa taacttgata tatcaattga cttatgttat actcgttatg
    19501 gtcagtgcca tatatggcgt ttctccttcc gcggaggcct ggtgcgaacg agcgtcaggc
    19561 ctcttttata ttgccggatt ttctgtcctg ttcaggactt ccccccttct gcgtggcttt
    19621 gctgtttaat cccccagtac agttcgtgac cgatgaccag aaacagccct gccataattg
    19681 ccagatctgc aacattgaaa atgcctgtcc gtagcgaata ccctccgatt tgtacgccca
    19741 tattcataaa atcgactaca gacccatcac gaaagattct gtcgatcaga tttccgatac
    19801 cgccaccggt gatggttgca aaggcgacaa atggcagtgg tttaattaat ttcgcaagaa
    19861 gaagataagc gaccaggacc cccaaaatca gagcattgac tcctaccata atcaaggatc
    19921 gggttgccgt cggtaaagca gcacccagac tcaggaaagc tcctttattt tcggcgtatt
    19981 gaatgcggat catatcaccc agaaaagaat aacagtttcc tgaatgtttc aatgttctga
    20041 tggcatgcca ttttgtcccg tgatccaaaa gaagaacggt tgcaacaatg gctatgatga
    20101 taattatttt gcgtttaatt gtcatactaa ctgcctttta tagtaaaaaa catttgacct
    20161 cttctgtttc aactgtctta aaccaatgcg gttgaggcat ggagacattt caaaccctga
    20221 attcgggcgg caacgagcat gaactgacag ttaataggca gtcccacagg tactgaattg
    20281 aatctgtttt gaagtccata cagcaaatac gggtttattc acctgtgcaa atggctgatc
    20341 ggtagacaaa ggtcccggcg tgccgcataa accgtcaggc tacaagtcac cggttggcgg
    20401 aatcggtggc ttcagcatta attaagactc tcctttttca gaagccccta ctctttgctg
    20461 aatgtcggca agaacggtgc gtgcaaaaga ctccatattg ggattctttg tccagtcaat
    20521 aaacttctgg atgctttcgc attgagcggc ggtaccatgg gttttcaata tctgcagtgc
    20581 atgataatga acggcttctt ccagtccagt ccgcgccagt gcatcggcaa acaaatccag
    20641 atgatctgct gcgaaaattt ccggaaatcc gtagataatt tctgttctgt ccgcatccgt
    20701 tgcttcaggc agaaaccaaa gttttgccgc atcctccacc gcttttccat ctcctttgcg
    20761 catcagaaca agaatggcaa ttttccggac atgcagtgct tcatcctgtg acaactgctt
    20821 cagtagatcg aaagcctcag gagtttcaat ggagccgatc aggtgtgccg cacagccacg
    20881 ggtggcaata tctttatccg gcgcagtcaa ttcctttaaa cggttaaagt cttctttttt
    20941 tacatgggtt ccaagagaga cgacagcaag cattttggcg cttgccggct gctcaggatc
    21001 agccagaaca gcgaagattc cttcaagtcc ttcgccgccg ggtgctgcca tctgctgggc
    21061 aagcataaag cctttgtgca gatccatggt tttaggatcc atagtggcaa cagcggtcca
    21121 ctgctccatg aggctggtcc cggaagaaat cccttcggcg accttggttt cctgtgcggc
    21181 agcaacaaca ttagtattag cgccgctgtc tcccggctgc ggcttaatgc agccgtaaag
    21241 acacagcaac ggaaaaagaa gtgccagcgt tttaattttc aacagtggtg aaagagacat
    21301 gagaaacctc cagttaattg atgtcgtgtt gaatatcagt tatttgcacc tattgtattc
    21361 atgagagccg taaaagaaca atgttttttt aataccctcc ggattgctat tatagtcgaa
    21421 atgcaaaacc ataagcgccg gaagcggtat gagtgcggag tttttacatg gatgactatc
    21481 agaaacagtt caatcagatt atagatgagg tgcgcctgct tgcaaaaaaa catgctcgag
    21541 gaagaccgcc ccatatcctg ttatccaaag aaacagcaga gaaactgctg cagattgacg
    21601 cattgaaaat gcggactgag aaagaggcgt gtgccgcaaa tatgcctccg gaacaggagt
    21661 cctgtaataa ggcgcaggac agcgcaggaa tgcctgcagt atcaggaaaa gatactgatg
    21721 cccttgccat ttctgtatct tcgcaggcag cgctggaagc gattgctgaa gaaattctta
    21781 tctgtaaaaa gtgccatctt agcgaaaagc gaaccaaagc ggttccgggc gaaggctctg
    21841 taaatgcttc gctggtcttt gttggcgaag cgcccggtgc ctccgaagat gcccagggca
    21901 ggccctttgt cgggagatcg ggacaattac tgacagatat tatagaaaaa ggcatgcagt
    21961 tgacgaggca ggatgttttt atctgcaatg tggtgaaatg ccgtcctccg gacaaccgtg
    22021 tgccttctcc cgacgaggtg cgtatatgtg agccttatct gctgcgccag ttggagattc
    22081 tcaagcctaa ggtgatttgc gcattgggct cagttgcggc acagacactg ctgaaaacca
    22141 ccgatcccat ttttaaattg cgtggccttt ggcatgacta ccatggcatc gcctgtcgcg
    22201 ttacctatca tccggcgtat ctgctgcgta atcctcctga taaaaagaag gcgtgggaag
    22261 acattcagga aatcatgcgc tttatgagag agaatgaaat tcccctgtcg ccccaatgac
    22321 ttccagaaca gccaccatga ccaatgcatc ttttgtcgcc cctcccttac gctggagacg
    22381 ctcattgttt ttccgggttg tcctcctgtg cgccgtactg ctgatttgcc ttctggcatc
    22441 ggtcatcgtg atcacccacc acttttttca ggaaatcatg cgtcagatgg aaacgcaggc
    22501 tatggagatc gcgcacagtc tggagattca aatcgaagag ggatttgacg gcgattacga
    22561 agcgctgggc agtaaattaa tggatctgta tcacggagtg gacattaaac tggatgaaca
    22621 aaccggagac ttgggtgcgg caacctttac gctgcaaagg cttgatgatg gttctattgt
    22681 tcgcatggcg cgtgtgccga tcgtcaataa tgatcagcca atggtcatga ctgtcattat
    22741 gaatattgcg cccaaaacgg aaatattgcg ggcattccgc aatcgtaatc tggttattct
    22801 gataattgtt tttctggtaa ctttagggtt tatggtctat ttcatcggca aagcactgcg
    22861 ccctcttgct gttctatctg aaagctgcgc tgctgtcact gaagggcatt tgcggcctgt
    22921 caatacgcaa ggggctgtcg gagaagtgct tcttctcgaa aataccttta accgtatgat
    22981 caccagtctc cacgaaaagg agctgatgga agcgaagctc cgtcaggcgc aaaggctttc
    23041 ggcgctgggc aatcttgctg ccggcatcgc ccacgatgtc cgcaatcctt taaatgctat
    23101 caaactgctg tcaagtcacg ctgcagcgct tctggaaaat gacaaccacc ctgccgaaaa
    23161 atcattgaag accgtgagcc gggaagttga ccggctggaa gaaattgttt ccagtttttt
    23221 gtcgcttgcc cgcgagactg aaatgcatcg tgagtttgtt gaaattgacg gtcttgtggc
    23281 ggaatgtgtt cgtttatttc aaaaagaggc ggagtcacgg agcgtcagac tgactcatga
    23341 tctccgctgt tccgggctga acctttatct ggatgcgaaa cagtgtacgc gtgctgtttt
    23401 aaacgtgctg atgaatgcga tcgatgcctg tccgcccgat ggtagagtac gtgtgtttac
    23461 gcgtcgaaca acaacgcact gtgaaattga gataagagat gatggcccgg gtatcccgaa
    23521 agaggtgatg gatactgttt tcgatcccta tttcactacc aagccgggag gtacagggct
    23581 ggggctgtcc attactcgca gtattgttga agaacatggc ggcaccattc aactttcggc
    23641 ttcctcagac agaggatgtc aggtgctcat cgtttttcct ctggataaag ctgctggagt
    23701 agttcaatag aaaacatgca gaaacaaaga attcttgtaa cagaagatga tcaggtgcag
    23761 cgggagatca tcgccgatat actcgaatat gccggttatg aagtgcggcc ctgctcctca
    23821 ggagcggagg cgcttaaagc gttgcaggag gaaaattttc atgtcctgct taccgacatg
    23881 aaaatgccgg gcatggacgg gttggaattg ttgcagcagg caaaaaggca gtgtcctgat
    23941 ctggatactg tggtgatgac ggcgcatgcc accatcaaaa cggctgtgac tgccattaaa
    24001 gagggtgctg ctgattatct tgaaaagcct tttggaaaag atgagctgtt gcttgtgatg
    24061 gatcggacgc tcgagagagg acggctcagg cgcgagaatg cccttctttc ccggcttgta
    24121 gatgattctg tttctctcgg taatatcatc ggtcaaagtc atgcaatgcg ggaagtgtat
    24181 gagcgcacca ggcgtgctgt gcaggtgccg agtacggtgc tgatacaggg cgagtccggt
    24241 actggtaaag aactgatagc ccgccatatc cacttcacgg gtccccgggc gaagaagcca
    24301 tttatagttg tcaattgcgc agctattcct gatatgctgg tagaatcgga acttttcggc
    24361 catgaaaaag gctcgtttac aggcgctgac agcatgcgca ccggaaaatt cgaattagcg
    24421 gatggtggca cgctgttcct ggatgagatt ggcgacatgc gtctggaaag ccaggcaaag
    24481 ctgctgcgag tactccagga cggactgata gaaaaagtag gaggctcaaa aagccggaaa
    24541 gttgatgtgc gcgtcattgt tgcgaccaat caggatttga aacgatgcgt tgccgagggg
    24601 actttccgtc aggatcttta ttaccgtctg gaagtgcttt ccattaccat gccgcctctt
    24661 cgggaacgta tggaagacct tcctttgctg atcaatcatt tcaaaaataa aattggcagg
    24721 aaactgaata tttctgttcc ggaagtgggg ccggatgtgc tggaagcctt ccggtgttat
    24781 cgctggcccg gtaatgtgcg agagctggag aataccctgg aggaagcatt tatccttgcc
    24841 cgttctcccc tgcttcgaaa gcaggatctg gcttcgaaat ttcaaagccc ggagcccgag
    24901 gtctcagaca tcaaactgcc gccgggggga cttgatcttg aagagctgga gcaggaactg
    24961 atccgtcagg cactggaacg cagcggaggc agcattaaag cggctgctcc tgttctcggt
    25021 atgacttata aaacgctgca ataccgtctt aaaaaataca atatcgaccc gaaaggcgat
    25081 gccgaagaca ccggagaata aatgacgcga tcaggaaaaa agaagcggga gcgttatatt
    25141 tcaacgctcc cgcagtaaat ttaacctgat gaagaaatta ttgggcaagc agaccgatag
    25201 cgttggcgat tagagcattg cagggaatat caccttgcgc ctcatacttc tggaacgcaa
    25261 cgtggccatc cataaacagc acattgcagc cgccgggaat atgattgaac agctgatccg
    25321 ctcctgtgtt ggagagaagg tcggacataa tcagaatctc actttgcgcc ttgttgcttg
    25381 ccgcagggtt gttaatgtcc gtgatcataa aacgttccac accttcgcga agcctgtaaa
    25441 tagtatctga agtgccgttg ccgctgttgg gataggcgaa gggacctttg agatcagagt
    25501 ccagcgcttc cggtttcggt ttccagtcgt ctccgaccgc agtgatttta aaataggcag
    25561 atgcaacctg gttggatact tccagagttt tactgccaac agtaagctgg aacacatcag
    25621 aggggctgat ggtcttcatg ctggctttat ctacaataaa accaagatag aaataactca
    25681 tgtcgggctg ggcgatcatc ccttcatact gaaggcaacc cggttcctgg gctgagattt
    25741 cgggcgtcat cattttaacg ccaccttgac gggcgtaccc gggcggattc tttaggcgtg
    25801 tacatggtca cggtgaatga tgagcgggaa aaccagtgga tcagacatac ttttggcgtg
    25861 ctcgataaaa gcatttacat cggtgccaat gatattgatg aggaaggacg ctgggtgtgg
    25921 caggaaaacg gcactcagtt ctggagtgga gtcgccgacg gaagtgcagt aaatggtcgc
    25981 tacagcaatt ggaacagcaa cgagcccaac aactacaatg atcacgaaga ctgcgggatg
    26041 atgtacggtt cctccggaaa gtggaacgac ctgtccggca cggcgctaaa tcctgtactc
    26101 ctggagaaaa atgcaggtgt ggtcattgtc gaaggaccct tcccggcaaa tgctacgata
    26161 cgtgccggaa agaaattcca gtttcaagtg gatgttcttc aatcctgggg cacaatatcc
    26221 taccaatgga cgcataacgg cgtggatatt cccggtgcaa cttcggacgt gctgaatttt
    26281 gaccctgtgg tcgaagaaga cgcgggactg tatgcctgca gactgacaga cggcacgggc
    26341 gcccatgcgg aaacagggac agcggcactt tccgtgacac ccggcggcgt catgccgttg
    26401 ctgcattcag cttccttgct gcttttggtt gcgcttctta tgagtattgt tggtgccggc
    26461 gcactctacc ggagaaatgc tgccttataa aaaaagcggt ggcatgaatg ctttaatcag
    26521 ctgaggcgtt gcgaaggcgg cgcctcagtt cttttgcact ggagatgggt ggcagacaac
    26581 ttcgatgcaa acagaggtat acggcgctgt gtgtgctctg atttgtctgt ggaggggctg
    26641 tggcatcatc agaagattca ggatctcttt cgagtttaag cagagtattc agcggcgcct
    26701 cctgtcgcag cacttccagt aacgcttcca aatccgcagc agtgcatcca gggcgaaggg
    26761 ttgcttctgt aaatccgtgc tgcagtgtgt cggcaaagag tgccagatgc aggtggccct
    26821 gaacggcatt ttgtgcgctc ggagcgcctg tctgccacac agttagtgca gcgttttcaa
    26881 aagcaggttc atcacagtgc catgccagtt tcataagtcc cagtgccgct gtgcttgcag
    26941 cagatacttc cgctgcatca tgcactgtct tcatgcgtgc gaaaagatgc ttatgctgct
    27001 cgccggtagt gaaaaaaccg ccattctttt tatccagaaa aagatgaatc atacgctccc
    27061 ctgttccctt tgcaagagca agccaccgcc tgttaaaagt gcattggtag agatctaaaa
    27121 aggcattggt taccgtggcg taatcttcca gataagccgg atgcgatgcc ttgccgtcac
    27181 tccatatcct gaagagccct tcatcggaca gcagatgcgt ttctacaaag tgccccgctt
    27241 tttctccggc atcccggtaa acatcatttt tcagataaaa ggctgccttt gccagagccg
    27301 ttatcatgag tccattccac gaggctatga ctttggtgtc aagacctgga cggatccggc
    27361 ggcttcgttt ttctaaaagt atttttctga ttgtgtccca cggttctccg atgatccacg
    27421 cctttgcaga aagaatattc aatccctcat gccccggttc atgagactca aaattgcctt
    27481 tttcgctgac agaaaaatgc tttttcaaaa atgccagatg ctctgcgctg agcccctttt
    27541 gcagctcctc ataagaccac agataaaatt taccttccaa gccttcactg tctgcatctt
    27601 ctgaactgta aaaagcgcct tccggactgg tcagatctcg aagtacatag tttaaaatgg
    27661 actcagtaat atgcctgtgc ttctcttttg cagtactctg ccacgcttca aggtaagaca
    27721 aaacaaggtg ggcgttgtca taaagcatct tttcgaagtg tggcacttgc cattgcgcat
    27781 caacagaata acgatgaaaa ccgccgccca gatggtcgtg tatcccccct gccgccatac
    27841 actccagcgt tttttccgcc atatgaagat aatgaggtat ctctcgcctt ttcccgtttc
    27901 gaagcaaaag ggcaaccgct gcatgcgacg gaaacttggg ggcgtcgccc cacccgcccc
    27961 attgggaatc aaaagttgcc gcaagcacag ccaatgcctg atcgaccagt gtttcaggtt
    28021 cgaccatatg gctggctggg actaccgtaa aatgatgttt taaacggtct gtcagtgccg
    28081 ctgcctgagt ctgtatgcgg cttctatcag aacgccacag gcgggcgatt tcctcgagta
    28141 tctggctgaa tccgggtatg ccatatcggc ttgttggcgg gaaataagtc ccggcataaa
    28201 atggctccag atcaggagtc agaaaaatgc tcgcaggcca cccgcccctg ccggtcagtg
    28261 ccacgactgc gctcatatac agatcgtcca gatccggtct ttcttcccgg tcaactttta
    28321 tattgacgaa gaaacgattc atcagagagg cgatttcagg attatcgaaa gattcgtgcg
    28381 ccattacatg gcaccaatga caggcggcat agccgataga caaaaaaatg ggcttatctt
    28441 ccagctgcgc ctt
//
LOCUS       DAOPWB010000023        28044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1659_length_28044_cov_5.467434, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000023 DAOPWB010000000
VERSION     DAOPWB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 28044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28044
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1659_length_28044_cov_5.467434"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKN92_03695"
     CDS             complement(<1..222)
                     /locus_tag="PKN92_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47928.1"
                     /translation="MKKKGGQFLSGHQRSWVWGRNPVLEIIRMGTWPVRECYFSDTLS
                     SNEYPDLSEKLQTWGIIPAYVSADRLKTLC"
     gene            complement(252..935)
                     /gene="queC"
                     /locus_tag="PKN92_03700"
     CDS             complement(252..935)
                     /gene="queC"
                     /locus_tag="PKN92_03700"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNZ47929.1"
                     /translation="MKLKQAAVVLVSGGLDSTVLLHAQRPLYENIHALSLSYGQRHMK
                     ELDCARYQAKRAGASFTQADLGELGGLLKGGSSLLSGGAEVPALVSIAPEQRDQPSTY
                     VPNRNMMLLSIAAAFAEVKGAQDVFYGAQAQVEYGYWDCTSDFVESMNAVLALNRRNP
                     VRIHAPFMHFTKEEIVKKGLELHVDFARTWSCYRGDVKPCGVCPTCVERNMAFQALHM
                     QDPLLTGAP"
     gene            complement(1015..2331)
                     /locus_tag="PKN92_03705"
     CDS             complement(1015..2331)
                     /locus_tag="PKN92_03705"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ47930.1"
                     /translation="MTKLAICGGKAVVKKSKKWAKWPISDAADAKLVAKITTSNRWSF
                     DGPVEWEFAEKFTAYQGAKYGMCVANGTIGIQIALEALGIGAYDEVIVPAMTWQATAA
                     ACVDVNAIPVLVDIEPDTWNLDLDAVEKAITPKTRAVIVVHLYGSMTDMTRLQKICKK
                     HNLFLIEDCAHQHGSFWKGQGVGSLGDISSWSFQESKVLSSGEGGFNMCKTRELFYKL
                     YSLRNCGRPYPADPAVFGLTKPANMQQYSLQSGNYRLTEWQAAILLGGLKRLDAQVKQ
                     RDINAIYLNSLLAGIPGILPMYRRPEITQQSYFNFAFRLDTKELKVDNKQFSVALNAE
                     LGLVDAFEPPYEPLNNCGLFRPQTKARHNLSKAYWKAIDITKYKLPVATEAHEKSGVT
                     IHHQLLMNSRSDMEMVASAVKKIVDNIDQLRAVKPGQLKKYQALSH"
     gene            complement(2524..2997)
                     /locus_tag="PKN92_03710"
     CDS             complement(2524..2997)
                     /locus_tag="PKN92_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNZ47931.1"
                     /translation="MHKTDRYVTAGGVVVNQKGELLVLERHVKRNGSVVHELRLPKGH
                     IDAGETLEQCALREVGEESGYWNLRIISYLGEVHSCFQRDHKIIERTEHYYLMTFIDT
                     DETRGVAQPVGEEEALFVPRWVLPSEICKTMTYSSEKEIVCRAESYLKQRNTGRL"
     gene            complement(2999..5467)
                     /locus_tag="PKN92_03715"
     CDS             complement(2999..5467)
                     /locus_tag="PKN92_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ47932.1"
                     /translation="MAMLIELILRKAAQEAIRLGNRYLCTEHVLLAMSMNSEARQLLT
                     QCGVNPDTLKEHLKLYLETEVEMVSGSNNEEVLPTPAYREMLERAKKHSQREMERAHE
                     REGKENGKGEEDGKTRLLGLLLAMMDQKDSFASYILSSYDISSHTVQASVMTGGVIRA
                     TWLKELCDYYDDDSGSDDPEDDDEGVELCNDDLEDEDGREGEDYRQWHRERESDLQLS
                     RCTDSLTMRAADGKIEPLIGREQELKRALRVLCRRKKNNLLFVGEPGTGKTALAEGLA
                     LLLFQSRFQETPVRVPEELRSKEVISLDMAALLAGAKYRGEFEERVKEILDALIARGN
                     VILFVDEIHTMVGAGATVECTIDAVSLMKPALAQGTLRCIGTTTYKDYKAYIENDHAL
                     SRRFQKIDLAEPTAQESYEILKGLQKDYEAHHGVSFSDETLRTAVDLSVRYMNDRFLP
                     DKAIDLIDEAAAMIRTAEEGRTKNVLPGDIEKLVSEYAGIPELRINVTEQKRLARLEE
                     DLKANVFGQDEALFHLARAIKRSRAGLGAPARPVGCFLFTGPTGVGKTEAARQLALCL
                     GNHFARYDMSEFMEQHAVSRLIGAPPGYVGFDQGGLLVDEIRRNPYTVLLLDEIEKAH
                     ADLFNILLQVMDNATLTDNNGRKADFRNVVLIMTSNAGARELVSTTIGFTSSAEDSRS
                     KSEKAIEKLLSPEFRNRLDATITFNHLNREVVLMIVDKLIRQLNQQLEERRVVLTVSE
                     KAKTKIADEGYDPKFGARPLARFIANEIETPLADEILFGKLQKGGALRIEVDKTSGEF
                     HFLFPDAELPAKSGRLRKIPAKAR"
     gene            complement(5510..5821)
                     /locus_tag="PKN92_03720"
     CDS             complement(5510..5821)
                     /locus_tag="PKN92_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNZ47933.1"
                     /translation="MSSCTSCEVKDQTNTAQLPEWGKRYKVILLNDDVTTMDFVVMIL
                     LDVFLKSFDDAVELMFQIHYEGMGIAGVYVKEIAEAKQEQVRQLAAAAGFPLRCTLMQ
                     E"
     gene            5996..7180
                     /locus_tag="PKN92_03725"
     CDS             5996..7180
                     /locus_tag="PKN92_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNZ47934.1"
                     /translation="MTQRQVYLDNNATTPLHPEVQKALIDALPLFGNPSSMHGYGREA
                     RSRTEEAREKIAAFIGALPEEIIFTGSGSEANNTVLSITGCPATECSCDRGQRTHIVT
                     TTIEHPCVLETSGCLSGRGTPVTYIPVDQYGIVDMDALRASITDHTGVVSVMMANNEI
                     GTIQNIKELAKLAHEKGAWFHTDAVQAVGKVPINVRDLDVDFLTLSAHKIYGPKGIGA
                     LYVKRGVPFCPLIRGGHQERGRRAGTENTLGVIGLGKAVEMRSLEMAEESLRFHAMKE
                     ALRKGIEDLIPDVRFNGHPDQSLASTLNVSFAGAEGESILLYLDLEGIAVSTGSACAS
                     GSLDPSHVLLATGVPVEQAHGSIRISMGRENTMTDVDYLLDKLPGIIARIRDMSTVYR
                     RK"
     gene            7185..7790
                     /locus_tag="PKN92_03730"
     CDS             7185..7790
                     /locus_tag="PKN92_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNZ47935.1"
                     /translation="MSAHQSWAYTEQVKDHFVNPRNVLREENYEEDGKGLVGNIQCGD
                     EMLVLIKVDRNTDTITDCKWRTYGCASAIASTSAMSEIVIGMPLDEAFKVGPKQITAA
                     LGQLPNNKIHCSVLGDKALRAAINDYYERNGRHDKVVREQARVVCTCMNVTDLEIEEA
                     VLEGARTFLELQEQTKMSTVCGKCRDEAETLLKVYMQKHFG"
     gene            complement(7814..9244)
                     /locus_tag="PKN92_03735"
     CDS             complement(7814..9244)
                     /locus_tag="PKN92_03735"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNZ47936.1"
                     /translation="MSTMDESTLRDLTEQYGQSHLFDFWEDLNHNEKAILLRSISEVK
                     FPEMKRLIESLVITEPPAEYYDEIKPVDLVAPNDRRAREAGDAGEALIRAGKVACFTV
                     AGGQGTRLGFDGPKGSYPIGALTGHSLFQYHAEKIRGLQRYYGTVLPWYIMVSPANGD
                     ATRAFFKQQNYFALDPANILFFEQQMIPCVNEKGKMMMETPFRLAVSPGGHGGSVQAL
                     VEKGILADAASRGVEILSYFQVDNWAVQVADPLFIGYHALQQSDMSSKCHRRNSPREA
                     VGVHCLCDQVYRVIEYSELDIYPQLLQVDASGDAVFSAGNSGIHLLNVSFVQDIYDHF
                     EDFPWHKAFKKIPCIREDGTLFSPDAPNGYKFETFIFDALRFARNPLALEIQRLGEYT
                     PIKDYDGVNSVVAARQSMTDYWSGWMKGAGIAIPEQSGSNAAWQLEISPAWAVNREEF
                     AERAQTLPKSFDKSTAILADGTILGK"
     gene            complement(9470..10597)
                     /locus_tag="PKN92_03740"
     CDS             complement(9470..10597)
                     /locus_tag="PKN92_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNZ47937.1"
                     /translation="MKIKASYVIIAVLLLLSVFILFAANQHSLSRYNNVTFDDGLSPP
                     IDVSMEDAPKIRLEKDSFDFGVIPRDRTTTKSILIFNDGKSPLEIRSLLTSCGLCTRA
                     AMADGKKRIPPGGSAELNITMLPSGVYGFYSKKTVSIGSNDPRHAKVNLEVEARVDPE
                     FVLEPETMEFGTVEQGVEAEASQRFRSLIDQPLSVKEVAFYVENEKPPVCPPLDLSFK
                     MVPEEEWRTPGRSEYIITARLNSAHSPGKFEDYFYIVTDLERFDAYRTPATGEVTAPF
                     SIPMEHLNGGINIHYPEHQAELTIHCTEAFSVSMPDGESELLAGSFEPLAPDRWIARF
                     SARADMPPGFNRSSVTLLIHTAAKTYRQCLPVFVSMPGAVR"
     gene            complement(10601..11611)
                     /locus_tag="PKN92_03745"
     CDS             complement(10601..11611)
                     /locus_tag="PKN92_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNZ47938.1"
                     /translation="MKYYLGLDAGGTKTFCLVGDASGHVLGFGAGGPGNYEVAGVPAA
                     AVEFQKAIDGALETAGLKLNQISAIGLGVAGADLPEDYVMLEKELLYPIMGDIPRTFE
                     NDSMGGLRGGTKDPFGIVIACGTGCVCAGVNRAGKKTRVGGINEDFGDMVSGSSIGIE
                     GLKAVWRSRDGILGETRLTEKFVTRSGCPDIEALFHEMYYGRITYADLEPMAKLVFEA
                     AWEGDQIACSILEWGGKYLADMVTAVARKLEMCRDEFDIVMTGSVFKGQSPVLKDVMT
                     ARIHRECPGARTVHALYEPVVGALLLGMEMDAPVTDEKYAVLSRSLDEAALKFGISFQ
                     AE"
     gene            complement(11697..12689)
                     /gene="ybgF"
                     /locus_tag="PKN92_03750"
     CDS             complement(11697..12689)
                     /gene="ybgF"
                     /locus_tag="PKN92_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNZ47939.1"
                     /translation="MKNYYWFMLAAIIVITGILPGCETMGGNQMQTTVYDMHRRMVKL
                     DRELDSSVSRLNESTATLIARTDQSDQQTRMLQGLLEENQAKLDALTRELASMKTTLY
                     RHWNLTTSGAPAGPPKDVSAGTVTIEGAQTPAVTPPSAPAVAPASAPAETPAPAAPPA
                     AAQTQTGKNEIFDSAPLPTETLTGAAQTAAPVQTAAEVPQSPAASDPRVLYQQAQRSF
                     ANEDYNTALRQFDEYLSQNPSSDLSANALFWKGKCLYSMDRYAESIQAFEQLRTNYGT
                     STKVPFAMHNQAVAHSRLGQLPEAARLMEAVIDQYPTSQAADQARTDLRKLRGE"
     gene            complement(12728..13336)
                     /gene="pal"
                     /locus_tag="PKN92_03755"
     CDS             complement(12728..13336)
                     /gene="pal"
                     /locus_tag="PKN92_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNZ47940.1"
                     /translation="MIPVHPAQNDTTQPPYRAKIGENESMVKFSKLMTVSLALLLVVG
                     MMAGCQRKKPLDTFTNLDDQSGLGSERTTGDGLPGMDLESLLFEPGSKYGLQTVYFDY
                     NSDALRGDAMDTLRSNAEKIKQVPGVVIQIAGHCDERGTQEYNMALGERRALAVRQYL
                     IQLGVSGDRIVTISYGKEFPAAVGSNEAAWSKNRRAEFNRAR"
     gene            complement(13726..14187)
                     /locus_tag="PKN92_03760"
     CDS             complement(13726..14187)
                     /locus_tag="PKN92_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47941.1"
                     /translation="MSAFTCSNCGFKGQSDDSTFSDTVLCPQCSYLVPLNTDPPLSPK
                     QTASAKNAAETPKSTVRGIVVLALLYTVFLAFLPLAYIYGQTGTPLCLLAFAIYLVIG
                     TVGGFLFLTGMNDPAYLTNWLRLPFKRSTATYAFLPLFVLGLLIAYFTYSP"
     gene            14586..16280
                     /locus_tag="PKN92_03765"
     CDS             14586..16280
                     /locus_tag="PKN92_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ47942.1"
                     /translation="MEPPATPHYFAAGVFRCALVLLLCCASVGHSDSADLSESPVEET
                     AETLTSSPEASPSSVETPGCDSDDNNASSAIESASDSNQPSIIFLDLIEAQRKAIQSN
                     PSLAAGAERVYQAKQMVAMTRSMYYPQIDLNYTYTWTWLPSSYTDPLLNYLDATEDLV
                     SDIRRQMYWYTISNQLPPQINRRDLRSWLNSADDLIDNSRKYLDSPYENAAINVTAGW
                     LLFDGFAREFANAMAKHGFQEAQAAYRDGQRILLDAVAQAYYGGQYAREQVVVAEASI
                     AFFERLRKEATARREVGRAATSDVLNFETALYAARGNLLRASREHELARIALALLIGE
                     EQGYLAETVQLAALEKETPDIMVTPDVDAMLDLAFACRPDLEQKELGLKRAQAAVRRE
                     YAKYAPQVAAVVRAQTANINETSFDADRITSTVGINVSVNLFSGGRRKAELTTAKHAR
                     KEAEWHIVASEQKVAADVRSALVDLKIAQEALTLQLEAASCVEKNRDLVEKEYNAGKA
                     MLVRLNQAQHDYVQAMGLLAQTRVNLHRCWQALHAATGVSLDIIRKEPLDMPETEE"
     gene            16510..17340
                     /locus_tag="PKN92_03770"
     CDS             16510..17340
                     /locus_tag="PKN92_03770"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47943.1"
                     /translation="MSFKSFLFSAVIASAALLTLAGCPPNAFYVLTVTAVPIEGGVVK
                     LDPERPSYFAGTTVTLTAVPAEGFTFRRWLGSGINTTINPTNKKIYADEAVAAEFQSD
                     VLIPGEGEPEEGEGHENFVKDGNFEYTTAGSTWQALSLTGMPIICETSTCGLLGGKGP
                     AHGLGWAWFGNSAQYGFEQATLAQQITLPVKREATLRFNVSVPLVTNPFTFSVSMNNV
                     VLWQLTEAEAMDFNAYQQVDLNIDDFATGRPAILSFSFSCTGEIGQESAVFIDDIQAY
                     "
     gene            17442..18140
                     /locus_tag="PKN92_03775"
     CDS             17442..18140
                     /locus_tag="PKN92_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47944.1"
                     /translation="MQIEDRVIHFSAELIHHPLPIERQQLQKLYYDLSQTRAAYDSID
                     ISVPTQAKFHSQRDKAQSIMLHLPERILMIEEWADIPLSTFAEKLHTAVPLVLQTQNI
                     AQILLHALTVRSTFALTHFDDARIFLLDHACHLAGRINPHFQRPLATGGLRFVFPETN
                     EHRGTLHVNIESFRHSANEIFVEVKGIFGRQPVDADHVGVITDNLRATRSFITENVFP
                     FLQQFDANPEQESP"
     gene            18362..19342
                     /locus_tag="PKN92_03780"
     CDS             18362..19342
                     /locus_tag="PKN92_03780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ47945.1"
                     /translation="MFHVFSLAKALINIHLLKKRTPLFLSWNITFRCNLRCQYCAACD
                     APPEELTTEEIKAGLDALWKLGTRWITFGGGEPLIRQDIGEIVQYARSRGFQCYLSTN
                     GSLLKSKLESIKPGVQHINLSLDGSETVHDQVRGKGSYAKAIEALELCKSSGTSVSLQ
                     CVLNRYNLDDFHKAVETAQHYKVPIMFQPATKWLSTSLESNPIAPDTAPYREAIQKII
                     ALKQRGAPIRNSYTGLKHLAHWPDPQPIFCAAGKSMAILEADGSMLACHQCQVKDFLH
                     GTAASGTWPEKFKNMPLPKHCAQCWCAPVVELCLFFSLKPEAMLNALRYR"
     gene            19400..21121
                     /locus_tag="PKN92_03785"
     CDS             19400..21121
                     /locus_tag="PKN92_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ47946.1"
                     /translation="MPIWLGINEDLFDSGVALSDGESVLFASNEERYSRRKNEGGFPH
                     RALESLFQYTGIAPQDVDYICMSGLMTPPPPVRLLPRSHQWLYNARREKKESRLRKLL
                     DDLTFYLPVSHTSNRSLLRKCSLPFLPFMTRMVLPHPLKKKPLRYVEHHIGHAAAAWK
                     LSGFEEALVLSVDGMGDGLSLLVLQCTPGQPMRRLWEVSSRDSLGLFFQVMAEVFGFV
                     SCRDEGKITGLAANGSSENVDFPFPFEYAEGRLRFTGQFGFGQRGIDWAKKMLGERYA
                     REDLAAWAQDNLEKYVVQIARDFLRKTGLRRLAVTGGVAANVKLNQRLHEIPEVEQLF
                     VLPNMGDGGLGLGALCAHDAVAPHRVPDVFWGESYTDAEIKSVLSQSGLPYRRVEAIE
                     NTIADLLVKEFLVARFTGRMEWGPRALGNRSILAKTTDSKVVTRLNALLQRSDFMPFA
                     PAVLAEDLDLYCLNTGAGRHAAEFMTVCFDCTEKMKQENPAIVHVDGTARAQAVSAGA
                     NPDFYKILKAFKDISGSSVLLNTSFNVHEEPIIRTPEEAITAFKKARLDYLAIGPFLV
                     DGFPICR"
     gene            21278..22513
                     /locus_tag="PKN92_03790"
     CDS             21278..22513
                     /locus_tag="PKN92_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HNZ47947.1"
                     /translation="MNKYFAILFLMLIVAFSADAGESPQFRGPWRDGRFDESKLLTTW
                     PEEGPPCLWTTHGLGHGYSSPVIADGKIYIPGMIDEATAGIFVLNLSGEIERVIPVGI
                     ETEDTQAPGPRSTPTLDGDRLYMLSGLGELFCLNIASGEKIWSVNILERFKGPNIMYT
                     LAESLLVDGDRVICTPGGADAGLAALDKYTGETVWTTPGFTDEASYCAPIIYTHKGRR
                     LLLTETANYLVCLDAKNGSFLWKMEHPTRDGIHAVTPLYLDGLIYYTGGYEAESGAVR
                     LSDDGSSYEKVWQDNLLDCQHHGVVLHEGYLYGTSHMRGNHLVCIEMKSGEIMWIAKD
                     VKQGVVIYADNMLYIYEGPTRGIVHLVKADPTAFHHAGSFRITEGDGRHWAHPAIAGG
                     ILYIRHGDAVMAYDLRSKP"
     gene            22722..24542
                     /locus_tag="PKN92_03795"
     CDS             22722..24542
                     /locus_tag="PKN92_03795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ47948.1"
                     /translation="MLFSYNAFKRIPFYSALILFAVSWGATALSVDNALDSARRRLAE
                     NYELSQLRSMTPQQIMEQLTEEECAAFANEHIRFQINCPAKVYVLYDSRFEEKGPFEA
                     FWLKDHAFERTDVQVSSDGDYFVGYARDFDAGAVGLGINSFSGNYLHYFIAVQPLRKE
                     DTLTISGIYPQSIRTAAFQKGVRPYTHPEWQPIDSYSPALEGATLLQVDARWRRTTKL
                     LDFFAETDYPASPRPDNIILTWSSDPRYTQSILWRTSEESRQGAVAYMKKKDYRHFTP
                     GAPVIKEAQSLRHESIHLLNQPVIYTHTATLTDLEPDTSYVYAVGDGSSDGWSALNEF
                     TTAPDKEAPFSFIYMGDVQVGYPRWRSLLERAWQDQPNARFYMLAGDNVNRGVDRNEW
                     DAFFAHTTGVFDHRVVVPALGNHEYIGGDPTLFEDFFVLPENGPENIAPEHCFSFEYS
                     NALFVIVDTNLLPEEYVGWLDETLGQSHAVWKFVMMHHPLYSSGINRDNVWLRDTLLP
                     VFDRHHVDMVLQGHDHAYLRTGPMRDNQKAERPADGTYYVVSVSGTKLYKLADRPYAD
                     VAYEHLSTYQVIDLQLNPPRLVYRSFDVDGNMKDEVIIEK"
     gene            24600..25544
                     /locus_tag="PKN92_03800"
     CDS             24600..25544
                     /locus_tag="PKN92_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNZ47949.1"
                     /translation="MSKGLFAQNIIAVIWDFDKTLSPHYMQTPLFQHYDVDESQFWKE
                     VQALPAYYKKAGINVHQDTCYLGHLLTYVRAGIMKGLSNALLREMGKKIDFYPGIPDI
                     FDRLKKILDSQKYQEIDLHLEHYVVSTGLAEMIRGSVVAEHLSGIWASEFIETPAAPA
                     FNPDTSPEPGEITQLAGCLDNTTKTRAIFEINKGVNKKENISVNDTIPESERRVPIKN
                     MIYIADGPSDVPSFSVICKHGGKAYAVYDTDSTARFSQVDDLQRSGRVDSYGPADYRE
                     GGHTDMWLKKYITAIADRIVDERRQALEKQIGKGPQHL"
     gene            25605..26561
                     /locus_tag="PKN92_03805"
     CDS             25605..26561
                     /locus_tag="PKN92_03805"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HNZ47950.1"
                     /translation="MNTLCLLASALLAIQSAFTMEDDGTALSLYENGNPVLVYHYGLV
                     KPAWPVPEHYTRGCYFHPLYGLDGEILTQDFPIDHFHHRGLFWAWPDSLSGDRKIDVW
                     ILKDARQHHVSWIKKEPGTESAFFALVNHWAFDGTPDTAIMEEKVTFEVAPEKENHRF
                     LDFTLTFTNVSDAVLTLRGAGTDNKGYGGFCFRPDAARKPFIFSSAQGVSEEDALMLN
                     SPWADISFPEKPGGNIYSGVAIFQHPGLPGFPHTGWILRHYGFLGQSWPHVRDHVMQP
                     GDSFTLKYRLVIHRGTGEDAGIASLFQEYLSECSSTNDTRTP"
     gene            26577..27599
                     /gene="queA"
                     /locus_tag="PKN92_03810"
     CDS             26577..27599
                     /gene="queA"
                     /locus_tag="PKN92_03810"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNZ47951.1"
                     /translation="MKSSDLDYKLPESLIAQYPAEKRDASRLMIIDRNSGRIQLDVFE
                     NMPDYLNPGDCLALNDTRVIRARLHGRKETGGAVEIFLLQELSDGGWSALVRPSAKVR
                     AGAKILFPAGLEAVVAETLPGGKRRVFFNGEQVISRLEEAGEIPLPPYIHRKESDHND
                     TTRYQTVYSKHPGAVAAPTAGLHFTENVFRKLAAAGIEKAYLTLHVGYGTFKPIQADR
                     LSDHQVDQEEYIFPEETAQKLNDTRHCGHKVVAVGTTCTRVLESCYSDGKYSGGNGST
                     DLYIFPPYTFRAVDVLQTNFHLPRSSLLALVCAFAGRELLMDAYHLAIKEQFRFYSYG
                     DVMLIR"
     gene            27748..>28044
                     /locus_tag="PKN92_03815"
     CDS             27748..>28044
                     /locus_tag="PKN92_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ47952.1"
                     /translation="MTIPMVDLRAQYHALRKEIEKAVLSVLERQQFRGGSDVESFEKA
                     LADFAGVKHALGVSSGTDALLLPLKTLDLRPGDEVITTPFTFFATAGAIVNAGGI"
BASE COUNT         6840 a         7329 c         6800 g         7075 t
ORIGIN      
        1 acagagcgtt ttcaaccggt cggcggatac ataagcaggt ataatacccc aagtctgaag
       61 tttttccgaa agatccgggt attcgttgct gcttaaggta tcgctgaaat aacactcgcg
      121 gacaggccag gtccccattc ttatgatttc aagaacagga ttgcgccccc agacccagct
      181 gcgttgatgt cctgacagga actgtcctcc tttttttttc aacagactac ccttttatac
      241 caatatcttt tttatggtgc gcccgtgagg agcggatcct gcatgtgcag tgcctgaaaa
      301 gccatattcc gttcaacgca ggtgggacac acgccacagg gcttaacgtc cccgcgataa
      361 cagctccagg tccgggcaaa atccacgtgc agttccagtc cctttttaac aatttcttct
      421 tttgtaaaat gcataaaagg ggcatgaata cggacaggat tgcgcctgtt cagtgcgagc
      481 acggcattca tgctctccac aaaatccgaa gtgcagtccc aatatccata ttcaacttgt
      541 gcctgagccc cataaaacac atcctgcgct cctttgactt cggcaaaagc ggcggcaatg
      601 gaaagaagca tcatattgcg gttgggcaca taagtagagg gctgatcgcg ctgttctggt
      661 gcaatcgata ccagtgccgg cacttcggca ccgccggaca gcaaagaaga cccgcctttg
      721 agcagaccac ccagctcgcc cagatctgcc tgcgtaaaag aagcgcccgc ccgctttgcc
      781 tgatagcgcg cacaatccag ttctttcatg tggcgctgtc cataggaaag ggaaagtgca
      841 tgaatgtttt catagagcgg tcgttgtgca tgcagcaata cagtggaatc caaaccgccg
      901 ctcacgagaa ccaccgctgc ttgtttcagc ttcatcagcg tttgcctttt tgaattaaaa
      961 aggcggtctg accacgtgtg atcagaccgc acagtatcat ttcatggccg caaatcagtg
     1021 agacagtgcc tgatattttt tcagttgccc cggctttacg gcacgcagct gatcaatatt
     1081 gtcgacgatc tttttaactg ctgatgccac catttccata tcgcttctgg aattcataag
     1141 gagctgatgg tgaatggtca cgccgctttt ttcatgtgcc tcggttgcaa caggaagttt
     1201 gtacttcgta atgtcgatcg ctttccagta ggctttactg aggttatgtc tcgctttggt
     1261 ctgggggcgg aaaaggccgc aattattgag cggttcgtag ggcggttcaa aggcatcgac
     1321 caatccgagc tccgcattga gcgcaacgct aaattgtttg ttatccactt tcagttcttt
     1381 ggtatcgaga cggaaagcga agttaaaata gctctgctgg gtaatttccg ggcgccgata
     1441 cataggcaga attccgggaa tccctgcaag aagagaattg agataaatgg cgttgatgtc
     1501 tctttgcttt acctgtgcgt ccaggcgttt aagccctcca agcaggattg ccgcctgcca
     1561 ctcggtaaga cggtagtttc cggactgtaa agaatattgc tgcatatttg ccggtttggt
     1621 caggccgaag acagcggggt cagcaggata ggggcggccg cagttacgga gactgtataa
     1681 tttgtagaaa agttcgcggg ttttacacat gttaaatccg ccttcgccgc tggacagtac
     1741 ttttgattcc tgaaaactcc aggaactgat atcgcccaga ctgcccacac cctgcccctt
     1801 ccagaaggag ccatgctgat gggcgcagtc ttcaattaaa aagaggttgt gctttttgca
     1861 gatcttctga aggcgggtca tatctgtcat ggaaccgtac agatgcacaa caataaccgc
     1921 tctggttttg ggggtaatcg ctttttcgac agcatccaga tcaagattcc aggtgtccgg
     1981 ttcaatatca acgagcacag gtattgcatt gacatccaca caggccgctg ccgttgcctg
     2041 ccaggtcatg gcaggaacaa tcacttcatc ataggcgccg atacccagtg cttccagggc
     2101 aatttgaata ccgatcgtgc cgtttgcgac acacatgcca tatttcgcgc cctgataggc
     2161 ggtaaatttt tcagcaaatt cccattcaac gggcccgtca aaagaccatc gattggaggt
     2221 cgtaatcttt gcaaccagtt ttgcatcagc cgcatcgctg atgggccatt ttgcccattt
     2281 cttggatttt tttaccactg ctttgccgcc gcaaatagcc agttttgtca ttatctgtct
     2341 ccttagattt ggttggattc agcacacgcg catcttgtca aggcggatcc cgctccagta
     2401 gcccggatgg gaccgcttgc tgaaactgac ggttaaaaag aattgtttca gtatacaccg
     2461 gctctgttat gcagtgcaaa aaccagccgg acaaatattc acatttgctg cgttcaacaa
     2521 cattcagagg cgcccggtat ttctctgttt cagataactt tctgcccggc agacaatttc
     2581 tttctcagag gaataggtca tggttttgca tatctcggac ggaagtaccc atcggggtac
     2641 aaaaagagcc tcctcctctc cgacaggctg tgccacccct cttgtttcgt cggtgtcgat
     2701 aaaggtcatg aggtaataat gttctgtccg ttcgataatt ttgtgatccc gctgaaagca
     2761 gctgtgcact tcgcccagat aactgataat cctcagattc caataaccgc tttcttctcc
     2821 cacctcgcgt aaagcacact gttcaagtgt ctcacctgcg tcaatatgcc ctttgggaag
     2881 ccgcagctca tgcacaacgc tgccgttacg ctttacatgt ctttccagaa caagcaactc
     2941 gcccttttga ttgacgacga caccgcccgc agtaacatac ctgtcggttt tatgcacgtc
     3001 acctcgcttt cgcaggtatt ttccggagtc gcccggattt tgccgggagt tcggcatcgg
     3061 ggaacagaaa atgaaattct ccactggttt tatccacctc gatacggagg gcgccaccct
     3121 tttgaagttt tccaaaaagg atttcatcag ccaagggtgt ctctatttca tttgcgataa
     3181 accgcgccag agggcgtgcc ccaaatttcg gatcgtaccc ttcgtcagca atttttgtct
     3241 tcgccttttc gctcacagtg agcacgacac gacgctcctc caattgctga ttcagctgcc
     3301 ggataagttt atccacaatc ataagaacca cctccctgtt caggtgatta aaggttatcg
     3361 tggcatccag cctgtttctg aattcgggag aaagaagttt ttcaatggcc ttttcacttt
     3421 tactgcggct gtcctcagcc gatgatgtaa agcctattgt agttgaaacg agctctcttg
     3481 caccggcatt ggaagtcata ataaggacaa cattccgaaa atccgctttt ctgccgttgt
     3541 tgtctgtcag ggtagcgttg tccatcactt gcagcagtat attgaagaga tccgcatgcg
     3601 ccttttcgat ttcatcgagg agcagcactg tgtacgggtt cctgcgaatt tcatcgacaa
     3661 gcagcccgcc ctgatcaaag cccacatagc ccggtggcgc gcctatgaga cgggacacgg
     3721 catgctgttc catgaattcg ctcatgtcgt aacgggcaaa gtgatttccc agacacagcg
     3781 caagctgccg tgccgcttct gttttcccca caccagtggg ccctgtgaaa agaaaacatc
     3841 cgacaggacg tgcgggagca ccaaggccgg cacgggaacg cttaatcgcg cgtgccagat
     3901 gaaacaacgc ttcatcctgc ccgaaaacat tcgctttcag atcttcttcc agccgggcga
     3961 ggcgtttctg ctctgtgaca ttaattcgca attcgggtat tcccgcatac tcagaaacga
     4021 gtttttctat gtcgcccggt aaaacgtttt tagtccgtcc ttcctctgca gtgcggatca
     4081 ttgccgccgc ctcatctata agatcaattg ctttgtcggg caggaaacgg tcgttcatat
     4141 aacgcacaga cagatctaca gctgttctga gtgtttcatc ggaaaaagac accccgtgat
     4201 gtgcctcata atccttctgc aatcctttga gaatctcata gctttcttgt gccgtgggct
     4261 ccgccagatc aattttctga aagcggcggg acagggcatg atcattttct atataggctt
     4321 tatagtcttt ataggttgtg gttccgatac agcgcaaagt cccttgagcc aaagcaggct
     4381 tcataagaga gacggcatca atcgtgcatt caaccgttgc gccggcgccg accatggtat
     4441 gaatctcgtc aacaaacaga atcacattgc cacgcgctat cagggcatcc agtatttcct
     4501 tgacccgctc ttcaaattcc ccgcgatact ttgctcctgc cagaagagct gccatatcca
     4561 gcgaaatgac ttcttttgaa cgcagctctt ccggaacacg gacaggcgtc tcctggaaac
     4621 gggattgaaa aaggagcaac gccaatcctt cggcaagggc tgttttccct gtccccggtt
     4681 ctccgacgaa aagaagatta ttttttttgc ggcggcacag cacgcgcagt gcccgtttca
     4741 attcctgctc ccggccgatt aagggctcga tttttccgtc tgcagcccgc atggtaagtg
     4801 aatcggtgca tctgctgagc tgcaaatcac tttcacgctc ccgatgccac tgcctataat
     4861 cctccccttc cctgccatcc tcgtcttcca ggtcatcgtt acaaagttcc actccctcgt
     4921 cgtcatcctc cggatcatca gacccggaat catcgtcgta ataatcacat aattctttaa
     4981 gccacgtggc ccgtatcacc ccgcccgtca ttacgcttgc ctgcactgta tgggaggaga
     5041 tgtcgtaact gctcaggata taggaggcga aagaatcctt ttggtccatc atggcaagca
     5101 acaggcccag aagtcttgtc tttccgtctt cttccccctt tccattttcc ttcccctctc
     5161 tctcgtgcgc cctctccatc tccctctggc tgtgtttttt tgcgcgctca agcatttctc
     5221 tgtatgcggg cgtaggcagc acttcctcgt tattgcttcc gctcaccatt tcaacctcgg
     5281 tctccaggta gagttttaaa tgctctttca gcgtatccgg gttcacgccg cactgggtga
     5341 gaagctgccg tgcttcgcta ttcatactca tcgcgaggag tacatgctct gtacatagat
     5401 agcggttgcc caggcggatt gcctcctgag cagcttttcg aagaattaat tcaatcaaca
     5461 ttgccatcgg taaagtactc catcaggaaa aaggtagttg ctgttctgtc tattcctgca
     5521 tcagggtgca tcgcagcgga aaacctgcag ccgcagcaag ctgacgcacc tgttcctgct
     5581 ttgcctctgc aatctcttta acgtaaacgc cggcaattcc cattccctca taatgaattt
     5641 gaaacatgag ttctacggcg tcatcgaagc ttttcaaaaa aacgtcgagt aaaatcatca
     5701 ctacaaagtc cattgtagtg acatcatcgt tcaacaaaat gaccttatac cgctttcccc
     5761 actcaggaag ctgtgcggta ttggtctggt ctttcacctc acacgatgta caagacgaca
     5821 taggctgcta tcctgtccag atgttttcat cgggtaaatg gtacttgagt taaactgtcc
     5881 ttatcaaccc attaaagccc ctgcatctga catggaatat tcattttgtt ttgcgtgttg
     5941 gacatccggg gtgtttctat tctaaaattc cattgattca acgggagtat ttattatgac
     6001 acagcgccag gtatatctgg ataataatgc cacgaccccc ttgcatccgg aagtgcagaa
     6061 agcactgatc gatgcgctcc ctctgttcgg aaatccatcc agcatgcacg gttacggacg
     6121 ggaagcccga tccaggacag aagaagcccg ggagaaaatt gccgccttta tcggtgccct
     6181 cccggaagaa atcatattta ccggcagtgg ctccgaggca aacaacacgg tgctttccat
     6241 cacgggatgt cctgccacgg agtgttcctg tgaccgtggc cagaggacac atattgttac
     6301 tacgaccatt gagcatccct gtgttctgga gacctcgggc tgtctatccg gacgaggaac
     6361 gccggttact tatataccgg tcgatcagta cggaatcgtg gatatggacg ctttgcgcgc
     6421 cagcatcacc gatcacaccg gtgttgtttc cgttatgatg gcaaacaacg aaatcggtac
     6481 gatccagaat attaaagaat tggcaaagct cgcccacgaa aagggcgcat ggtttcacac
     6541 ggatgctgtg caggctgtag gcaaggtgcc tattaacgtt cgtgatcttg atgtggattt
     6601 tctgacactt tccgcccata aaatttatgg tcccaaagga atcggtgcac tgtacgtcaa
     6661 gcgcggcgtg cctttctgcc cgctgattcg aggcggccat caggaacggg gtcgccgggc
     6721 ggggacagaa aataccctgg gcgtcatcgg tttgggcaag gctgtagaaa tgcgctctct
     6781 tgaaatggcc gaagagtccc tgcgttttca tgccatgaaa gaagccttga gaaaagggat
     6841 tgaggatctg atccccgatg tacgcttcaa cggacatccc gaccagtctc tcgccagtac
     6901 gctgaatgtt tcttttgccg gtgccgaagg cgaaagcatt ctcctttacc ttgaccttga
     6961 aggaattgcc gtgtccacag gttccgcctg tgcctccgga tctctggatc cttcccatgt
     7021 cctgcttgcg acaggtgtcc ccgttgaaca ggcgcacggc tccatacgca tcagcatggg
     7081 aagagaaaat acaatgacag atgttgacta tttactggac aaactcccgg gcatcatcgc
     7141 cagaatcagg gatatgtcca cagtatacag gagaaaataa agccatgagc gcacaccagt
     7201 cctgggccta taccgagcag gttaaagatc attttgtcaa tccccgcaat gttctccgcg
     7261 aagaaaacta cgaggaggac ggaaaagggc ttgtcggcaa tattcaatgc ggcgatgaga
     7321 tgctggtttt aattaaagtt gaccgcaaca cagacaccat aaccgactgc aaatggcgca
     7381 cctatggctg tgccagcgct attgccagca cgtccgccat gtctgaaatt gtgataggga
     7441 tgcctcttga cgaagcgttt aaagtcggtc ccaaacaaat caccgctgca ctgggtcagc
     7501 ttccaaacaa caagattcac tgttccgttc taggcgacaa ggcgctgcgt gcagccatca
     7561 acgactacta tgaaagaaac ggacggcatg ataaagtggt tcgcgagcag gcgcgtgttg
     7621 tttgcacctg tatgaatgtc accgatctcg aaatagaaga agcggtgctg gaaggcgcac
     7681 gcaccttcct ggaattgcag gaacagacaa agatgagcac ggtatgcggc aaatgccggg
     7741 atgaagcaga gactctgctc aaagtataca tgcaaaagca tttcggctga gcaccggatc
     7801 gcgtactacc ctgtcatttg cccagaatag tgccgtctgc cagaattgcc gtgcttttat
     7861 caaaactttt cggcaatgtc tgtgcgcgct ccgcaaactc ttccctgttc accgcccagg
     7921 caggactgat ttccagttgc catgccgcat tgcttccgga ttgttcagga atcgcaatcc
     7981 cagccccttt catccacccg gaccaataat cggtcatgct ctgccgtgcc gcaacgacac
     8041 tgttaacacc atcataatct ttaatcggtg tatattcgcc gagccgctga atttccagtg
     8101 caagaggatt tcgggcaaaa cgcagcgcat caaaaataaa agtctcgaat ttgtatccgt
     8161 tcggcgcatc cggagaaaaa agagttccgt cttcccggat acatgggatt tttttgaaag
     8221 ccttgtgcca gggaaagtct tcgaagtgat cataaatatc ctgaacaaaa gaaacattga
     8281 gcaaatggat tcctgaattt ccggcagaaa agacggcatc tcctgaagcg tcaacctgca
     8341 gcagctgggg atagatatcc aattcgctgt attcaatcac acggtacact tggtcgcaca
     8401 ggcagtggac gcccaccgct tctctcgggc tgttgcgacg gtgacatttg gacgacatgt
     8461 cggactgctg cagtgcgtga tacccgataa agagcgggtc cgccacctgc accgcccagt
     8521 tatccacctg gaagtaactg aggatttcaa ccccccggct ggcggcatct gccagaattc
     8581 ctttttccac cagagcttgt acactgcccc cgtgaccgcc cgggctgact gcaaggcgaa
     8641 agggtgtttc catcatcatt tttccctttt cattaacaca gggaatcatt tgctgttcaa
     8701 aaaataagat gttggcggga tccaatgcaa aataattctg ctgtttgaaa aacgcacggg
     8761 tagcgtcgcc atttgcaggg ctgaccatga tgtaccaggg cagcacggtg ccataatacc
     8821 gctgaagccc ccggatcttt tccgcatgat actggaacag cgaatgaccg gtgagggcac
     8881 cgatgggata actgcctttg ggtccgtcaa agccgagtct cgttccctga cctccggcaa
     8941 cagtgaagca ggcgactttg cccgcacgga tcagcgcttc tcccgcatcg ccggcttccc
     9001 gggcacggcg atcattcggt gcaaccaggt cgaccggttt tatctcatcg tagtactctg
     9061 caggcggctc tgtaatcacc agagactcaa tcagtctttt catctccggg aattttacct
     9121 ctgaaatact tcggagcagt attgctttct cgttgtgatt cagatcctcc cagaagtcga
     9181 aaagatgtga ttgtccatat tgttcagtga ggtcacgaag cgttgactcg tccatagtgc
     9241 tcattacagc tcctgaatat ttacaattca gcctttagtt aaaagtgttt ttgaaaactg
     9301 cggatcttaa ctctgcttcc cgtccacacc ccgaaaacaa tgaatcgcag gatagttaag
     9361 tcgacagaat atcccgtcat aaaatcagat gcgcctcctg catgcacgta ttatagcgcc
     9421 tgcccacagg ctgtaatcaa aagcgcaagc aaaagaaatt gcaagcaatc taacgtactg
     9481 ctccgggcat agacacaaaa acaggaaggc attgcctgta ggttttcgct gcggtatgaa
     9541 taagcagcgt aacgctgctt ctgttaaacc cgggaggcat atcagccctt gcggaaaagc
     9601 gcgcaatcca ccggtcgggc gcaagaggct cgaaagagcc ggcgagcaat tcactttcgc
     9661 catcaggcat tgaaacgctg aaggcttccg tacagtgaat ggtcagctcc gcctggtgct
     9721 ccggataatg aatattgata ccgccgttaa gatgctccat cggtatgctg aagggagctg
     9781 ttacttctcc cgttgccgga gtccggtatg catcgaaacg ctccagatcg gtgacaatgt
     9841 aaaaatagtc ctcgaacttg ccggggctgt gtgcggaatt taaccgggct gttatgatgt
     9901 actcgcttct tcccggcgtc cgccactcct cttcgggcac cattttaaag gataaatcaa
     9961 gaggcgggca cacaggaggt ttctcgtttt ccacataaaa agccacctct ttcaccgaaa
    10021 ggggctggtc aataagagag cgaaagcgct gagatgcttc cgcctcaacg ccttgctcca
    10081 ctgtaccgaa ttccattgtt tccggttcga gtacaaactc gggatccacc cgtgcctcca
    10141 cttccagatt gaccttagca tgccggggat cgttgctgcc gatggacact gtttttttcg
    10201 agtaaaaacc gtagacaccg gaagggagca ttgtaatatt cagttcggct gaaccgccgg
    10261 gcgggatcct cttttttccg tctgccatag ccgctctggt gcataaacca catgaagtca
    10321 gcagacttcg aatttcaaga ggactctttc cgtcgttaaa aataagaata cttttcgtgg
    10381 ttgttctgtc ccgaggaatt accccgaagt caaaggaatc cttctcaaga cgaatctttg
    10441 gtgcatcctc catagaaaca tcaatgggag gagacagccc atcatcaaaa gtgacgttgt
    10501 tatagcgtga aagagaatgc tgattggcgg caaaaagaat aaaaacagaa agcagtagca
    10561 gtacagcaat gattacataa gacgccttga ttttcatggt ttattccgcc tgaaagctaa
    10621 tcccgaactt taaagccgct tcatccaggg aacgggacag cactgcatat ttttcatctg
    10681 taacgggcgc atccatttcc atgcccagca gcagagcgcc gactacaggc tcgtaaaggg
    10741 cgtgaacggt ccttgcgccc gggcactccc ggtgtatacg tgcggtcata acatccttca
    10801 gcacagggct ttgcccttta aatacacttc ctgtcatgac tatgtcaaac tcatcccggc
    10861 acatttccag tttgcgcgct acagcagtta ccatatccgc cagatactta ccgccccact
    10921 ccagaataga gcaggcaatc tgatcgcctt cccaggcagc ctcaaaaacc agttttgcca
    10981 tcggttcgag atcggcatag gtgatccgcc cgtaatacat ttcatgaaac aatgcttcta
    11041 tgtccgggca gccggatcgg gtcacaaatt tttctgtcag tcgtgtttca cctaaaatac
    11101 cgtcccggct gcgccatacc gctttcaggc cttcaatgcc gatgcttgat ccgctcacca
    11161 tgtctccaaa atcttcgttg atgccaccca cccgcgtttt tttgcccgcg cggttaaccc
    11221 ctgcacaaac gcatccggta ccgcaggcaa tcacaatacc aaagggatct ttggttccac
    11281 cgcgcaaccc gcccatagaa tcgttttcaa aagtacgggg aatatctccc ataatgggat
    11341 aaagcaattc tttttccagc atgacatagt cttcgggaag atcggcgccc gccacaccca
    11401 gaccgatagc agaaatctgg tttaatttca ggcctgctgt ctcaagagcg ccgtcaatgg
    11461 ctttctgaaa ttcaaccgct gcggcaggta cgccagccac ttcataatta ccggggccac
    11521 cggcgccaaa gcccagcaca tgtccggaag catcacccac caggcaaaaa gtttttgttc
    11581 caccggcatc aagacccaga tagtatttca tttttactct ttccttaatt taaagaacaa
    11641 atcgggcgcg gaaagttttt tcccgcgccc gacactatta acaacacgac aagagattat
    11701 tcgccacgca gcttacgtaa atctgtccgc gcctgatccg ccgcctgact ggtcggatac
    11761 tgatcgatca cggcttccat cagacgcgcc gcttcaggga gctgaccgag tctggaatgg
    11821 gcaacagcct gattgtgcat ggcaaaaggc actttcgtgc ttgtaccata attggtacgc
    11881 agctgttcaa aagcctgaat ggactcagca taccgatcca tactgtagag acattttcct
    11941 ttccagaaca gcgcattggc gcttaagtca ctggaagggt tttgcgacag atattcatcg
    12001 aactggcgaa gggcggtatt gtaatcttca ttggcaaaac tgcgctgtgc ctgctgatac
    12061 agaaccctgg gatcgcttgc tgccggactt tgcgggactt cagctgctgt ttgcaccggc
    12121 gccgctgtct gtgccgcacc agtcaacgtt tctgtcggca atggcgcact gtcgaagatc
    12181 tcatttttgc ccgtctgcgt ttgtgcagcc gcaggaggag cggccggagc cggtgtttct
    12241 gcgggtgcag acgcaggcgc aacggccggt gccgaaggcg gtgtcacagc gggtgtctgc
    12301 gcaccttcaa tggtaaccgt ccctgcggaa acatccttgg ggggacccgc aggtgctccg
    12361 gacgtggtca ggttccaatg gcggtacaag gtggtcttca tgctggcgag ttcgcgtgtc
    12421 agcgcatcaa gtttcgcctg attctcttcc agcagccctt gcagcatgcg tgtctgctga
    12481 tcgctctgat ccgtccgggc gatcaacgtc gccgtgcttt cgtttaagcg agatacagaa
    12541 ctgtccagct cccgatccag tttgaccatt ctgcggtgca tatcgtacac cgtggtctgc
    12601 atctggtttc cacccattgt ttcgcaaccg ggcaggatcc ctgtaatgac gatgatggct
    12661 gccaacatga accagtagta gtttttcata gtctgtatct cacaatagtt tggctgcaag
    12721 gaacagttta tcgggcacga ttgaattcag cgcggcggtt cttcgaccag gcagcttcgt
    12781 ttgaaccgac tgctgcagga aattcttttc cgtaactgat cgttacgatg cggtcgccgg
    12841 aaacacccag ctgaatgagg tactgacgga cagcaagggc acgccgttca cccagcgcca
    12901 tattgtattc ctgagtgccg cgttcgtcac agtgaccggc aatctgaatc acaacgccgg
    12961 gaacctgttt gattttttca gcgttggagc gcagtgtatc catggcatcg ccacgcagcg
    13021 catcgctgtt atagtcgaaa tatacggttt gcagcccata cttggagccc ggttcgaaaa
    13081 gaaggctttc cagatccatt ccaggcagcc cgtcgcccgt ggtacgctcg gaaccaagac
    13141 cgctttggtc gtcaagattc gtgaaggtat ccaggggttt tttgcgctga caaccagcca
    13201 tcattcccac tacaagaaga agcgctaaac tcactgtcat aagcttactg aactttacca
    13261 tggattcgtt ttctccgatt tttgcccggt agggcggttg ggttgtatcg ttttgtgcag
    13321 gatgcacagg aatcatacca aaatcgtatc tttcacatca acccttcagg catgctttcc
    13381 atctgcaaat ctgccatatt cgcggcagga aggcagttga tgtggttgtt tacctggcag
    13441 agcaggtaga gaacagttct tgcgcaaaat cggttgttca gggtgctgct cccgctcgaa
    13501 acagcccgtt ccccgctgca atcttccgac tttgccccgt agtacggcta cgctgcctat
    13561 cttgtatcat aaaatacctg aaaaggcaaa aaagttcccg attgcaccaa taattctgct
    13621 gttcttccgc ttcggtaatt caaacgttat aattggaaac aaaagtgttt ccaaattatt
    13681 ccttcttttg ctgtcacaaa caagtggcag aaagtcatat tattctcagg ggctgtaggt
    13741 gaagtaagca atcaataagc caagaacaaa aagcggcaga aaagcatagg ttgccgtgga
    13801 tctcttaaag ggcagtctga gccaattcgt caaataggcg ggatcgttca ttccggttaa
    13861 gaacaaaaaa ccgcccacag tgccgatcac aagatatatt gcaaaagcaa gcagacaaag
    13921 gggcgttcct gtctgcccat agatataagc caaaggcaaa aacgccagaa acaccgtata
    13981 cagtaaagcc agaacaacaa ttcccctaac ggtcgatttc ggagtttcag ccgcattttt
    14041 tgccgatgct gtctgcttcg gcgacagagg cggatccgta ttcaatggaa ccaggtaact
    14101 gcactgtgga cacagcacgg tatcggaaaa ggtactatca tcactttgcc ctttaaatcc
    14161 gcaattgcta caagtgaatg ccgacatcac gaaatcctcc tcgattttct gcactggcac
    14221 aatttgtggc aaacgctcca cttttttgtt aatgactact ttatcctgtg tcccgcattt
    14281 aaatcaaccg gggaaaaaca aaaacccttt acttgtgtcg aagcctgtct ggtaaaatat
    14341 ttctcaaaaa ttacctctgt tcccttgcag cagggagaag actgcgcttt ttttgcctga
    14401 aaaaaacaaa aaaaacctca ctattgaacg tcgcacatca gtttagtata ttcttaagga
    14461 gcacgattga tataaatcag gatctgtctg ccatgttttt ttattcccgt tcagtttttc
    14521 ggcggagttt acttttgatt tctaacgcca gaccggcgct attaaaagga aaggagtctt
    14581 tttttatgga accccctgca accccgcact attttgctgc aggcgtgttc cgatgcgctc
    14641 ttgttttatt gctgtgctgc gcttctgtcg ggcacagcga ctccgctgac ctttccgagt
    14701 ctcctgttga agaaaccgcc gaaacactga catcatcccc ggaagcctca ccttcttccg
    14761 ttgagacacc gggctgtgat tcagatgaca acaacgcatc ttccgctatt gaaagcgctt
    14821 cggatagtaa tcagccttcc atcatttttc ttgatctgat cgaagcacaa cgcaaagcca
    14881 ttcaatccaa tccatccctt gccgccggtg ctgaacgtgt ttatcaggca aagcaaatgg
    14941 tggcaatgac acgttccatg tattatccgc agattgattt gaattacacc tacacctgga
    15001 cctggctccc ttccagttat acggatcccc ttttgaatta tctggatgca acggaggatc
    15061 ttgtttccga tatacgccgc cagatgtact ggtataccat ttccaatcag cttccgccgc
    15121 aaattaaccg gcgcgacctg cgttcctggc tgaacagtgc agatgatttg attgataaca
    15181 gcagaaagta tctggatagc ccttatgaaa acgccgctat caacgtaacg gcaggctggc
    15241 ttctttttga tggatttgcc cgtgaatttg ccaatgccat ggcaaagcat ggttttcagg
    15301 aagcacaggc tgcctatcgg gacgggcagc gcattctgct cgacgctgtt gcccaggcct
    15361 attatggcgg gcaatatgcc cgggagcagg tcgtcgttgc agaagcatcc atagcctttt
    15421 ttgaacggct tcgcaaggaa gctacagccc gcagagaagt aggcagggca gcaaccagcg
    15481 atgtactgaa ctttgagact gctctttatg ctgcccgggg caatcttctc cgcgcatccc
    15541 gggagcatga acttgcccgc attgctcttg ccctgctcat tggcgaggag caaggctatc
    15601 tggctgagac cgtacagttg gcggcattgg aaaaagaaac gccggatatt atggtaacgc
    15661 cggatgtcga tgccatgctg gatcttgcct ttgcatgcag acctgatctg gaacaaaaag
    15721 agctggggct gaagcgggcg caggcggcgg tgcgtcggga atatgcaaaa tatgcacctc
    15781 aggtggccgc tgtagtgcgt gcccagacag ccaatatcaa cgagacttct tttgatgccg
    15841 accgcataac ctcaacagtc gggattaatg tttctgtcaa tcttttttca gggggcaggc
    15901 gcaaagcaga actgactacg gcaaaacacg cccggaaaga agcggaatgg cacattgttg
    15961 cgtcagaaca aaaagttgcg gcagacgtac gatctgcgct ggtagatctg aaaatcgctc
    16021 aggaagcgct gacgcttcaa ttggaagcgg cttcctgtgt tgaaaaaaac cgggatcttg
    16081 tcgaaaaaga gtataatgca ggtaaggcaa tgcttgttcg tttgaatcag gctcagcatg
    16141 actatgttca ggctatgggt cttcttgcgc agacgcgtgt taacctgcac cgctgctggc
    16201 aggcacttca tgctgccacc ggtgtaagcc ttgatataat ccggaaagag ccgctggata
    16261 tgccggaaac agaagaataa aaacgccggt gtggttacct tttcaaccca actgatttgg
    16321 agatgcgaca tgtttaaatc ttcccgcaat gatacgctcg actttttcta tgaccttttt
    16381 gatctgctga atttcattat cgagattttc agaaatctgg gaaatctttt cagccgtcgc
    16441 taaacattcc tgaatctcct gttatttata tacggtttta taatttcaga cggaaaaagg
    16501 aattccgcca tgtcctttaa atcttttttg ttttcagcag tcatcgcttc ggcagccctt
    16561 ctcacattgg caggatgtcc gcccaatgcg ttttacgtcc tgactgtgac cgctgtacct
    16621 attgaaggtg gcgtggtcaa actggatccg gaacgcccct cttattttgc cggaacaacg
    16681 gtcaccctta ctgctgttcc tgcagaagga ttcactttcc gccgatggtt gggttcgggc
    16741 atcaacacca caatcaatcc gaccaacaaa aagatctatg cagacgaggc ggtcgccgcc
    16801 gaatttcaat ctgacgtatt gatcccgggc gaaggagaac cagaagaggg ggaagggcac
    16861 gaaaattttg tcaaagacgg caactttgaa tataccaccg ccgggagcac ctggcaggcg
    16921 ctgtctctga ccggcatgcc gattatctgc gagaccagta cctgcggact actgggcggt
    16981 aaaggtcctg cccatggtct gggctgggca tggttcggca acagcgctca gtacgggttt
    17041 gagcaagcga cactggcaca gcagatcaca cttccggtta aaagagaggc cacgctcagg
    17101 ttcaatgttt ccgtgcctct tgtaaccaat ccattcacct tcagtgtcag tatgaacaat
    17161 gttgtactct ggcaactcac tgaagctgaa gccatggact tcaacgccta ccagcaggtc
    17221 gatctgaata ttgatgactt tgcaaccggt cgtcctgcca ttctgagctt ttccttctcc
    17281 tgcacaggag aaatcggaca ggaaagcgcc gtattcatcg atgacattca ggcttactga
    17341 cgcagtaacc ctttcactcc tgctctctga aagtccggca gcagtgtata actgcctgtt
    17401 ccagttcgca caggtattgt gttttgggaa gaggaagtgc tatgcaaatt gaggatcgcg
    17461 tcattcactt cagcgcagaa ttgattcacc atcccctgcc catagagcgg cagcagcttc
    17521 agaaactgta ttacgacctg tcgcaaacac gtgccgccta tgacagcatc gacatttctg
    17581 tgccaactca ggcaaagttc cattcgcaac gggacaaggc ccaatccatc atgctccatc
    17641 tgcctgagcg gattcttatg atcgaggaat gggcagatat cccgttgagt acttttgctg
    17701 aaaaactgca tactgctgtc ccgctggtac tgcaaacgca gaatattgca caaattttgc
    17761 tgcatgccct caccgtacgg tccacctttg cgctcaccca ttttgatgac gcacgcattt
    17821 ttcttctgga tcatgcctgc catcttgccg ggcgaatcaa cccgcacttc caacgccctc
    17881 ttgccacagg cgggctgaga tttgtattcc cggaaaccaa cgagcaccgg ggcacgcttc
    17941 atgtgaatat cgagtctttc agacacagtg ccaatgagat ttttgttgaa gtcaaaggca
    18001 ttttcggaag acagccggta gatgctgatc atgtcggtgt tattaccgac aaccttcgtg
    18061 cgacccgttc tttcattaca gaaaatgtct ttccctttct gcagcagttt gatgcgaatc
    18121 ctgagcagga atcaccatga gcgttgccag gtgtgaatgg cctgggcaga tcaggaaatg
    18181 agccagcgca tagcgttaag ccgcagcagc gccacagcgt cgcatccgga gcgtcctgtt
    18241 tttccttctg catttcccag tctttcactt ttatttgact ctttcacatt attttcttta
    18301 aactgaaaca cttgtaatca agcaacttga agcattattc cgttataaaa cagggtacct
    18361 gatgttccat gtattcagcc tcgcaaaggc gctgatcaat atccacctgc tgaaaaaacg
    18421 aacccctctt tttctctcct ggaacattac ttttcgctgc aatctgcggt gccaatactg
    18481 tgccgcctgt gatgcccctc ccgaagaact gacaacagag gaaattaaag cgggtctgga
    18541 tgcgctctgg aaacttggaa caagatggat cactttcggc ggcggcgaac cgctcatccg
    18601 tcaggatata ggcgaaatcg tccaatacgc acgctccaga ggattccagt gctatctcag
    18661 caccaatggc tctcttttaa aatcaaaact ggaatcgatc aagcccgggg tacagcatat
    18721 caacttgagc ctggacggaa gcgaaaccgt tcatgatcag gtacggggca aaggcagtta
    18781 tgcaaaagct atcgaggcac tggaactgtg caaatcttcc ggcacttcag taagcctgca
    18841 atgtgtgctg aaccggtata atctcgatga ttttcataag gcagtggaaa cggcgcagca
    18901 ctacaaagtg ccgattatgt ttcaacctgc aacaaaatgg ctcagcacat ctctggaaag
    18961 taatcccatc gcccctgaca cggcccctta ccgggaagca attcaaaaga ttatcgccct
    19021 taaacaaaga ggcgcgccca tccgcaattc ctatacaggg ctgaagcatc ttgcccactg
    19081 gcccgacccc cagcccattt tctgcgctgc gggaaagtcc atggcaattc tggaagcaga
    19141 cggctccatg ctcgcctgcc atcaatgtca ggtaaaagac tttcttcacg ggacagcggc
    19201 ttcagggaca tggccggaaa aatttaaaaa catgccgttg ccgaagcact gtgcacaatg
    19261 ctggtgcgct cccgtggtag agctttgtct ttttttctct ttaaagcccg aagcaatgct
    19321 caacgcattg cgctacagat aaactattct gtaaatgtgt cacggcctgc attcaaatta
    19381 acagtttaac ggagaagcca tgcccatctg gctcggtata aatgaagatc tgtttgattc
    19441 cggtgttgcc ttgagtgacg gcgaatctgt tctgttcgca tccaatgaag aacgttattc
    19501 ccgtcgtaaa aacgagggcg gttttccgca ccgcgctctg gaatctctct tccagtatac
    19561 gggtattgct ccgcaggatg ttgattacat atgcatgtcc gggctcatga caccgcctcc
    19621 gcctgtacgg ctcctccccc gatcccatca atggctctac aatgctcgtc gcgaaaagaa
    19681 agaatcgagg ctgagaaagc ttctggatga cctcactttt tacctgcctg tatcccacac
    19741 ctcgaaccgg tcactgctgc ggaaatgctc gcttcctttc ctgcctttca tgacccgtat
    19801 ggtgctgccc catcctttaa agaagaaacc cctgcgttat gtggaacatc atatagggca
    19861 tgctgctgcc gcctggaaac tttccggctt tgaagaagct ctggtgctgt ccgtcgatgg
    19921 catgggtgac ggtttatccc tgcttgtcct gcagtgcaca cccgggcagc ctatgcgccg
    19981 gttgtgggaa gtatcttccc gcgacagtct gggactcttc tttcaggtta tggcggaagt
    20041 attcggcttt gtttcctgcc gtgatgaagg caaaataacc ggtcttgctg caaacggctc
    20101 gtcggaaaat gtggattttc ccttcccttt tgaatatgcc gaaggaaggc tccgattcac
    20161 gggtcagttc gggtttgggc aacggggcat agactgggca aagaaaatgc ttggcgaacg
    20221 ctatgcccgt gaagatctgg cggcgtgggc gcaggacaat ctcgaaaaat atgtagtgca
    20281 gattgcccgg gattttttaa gaaagacagg gctgaggaga ctggcagtca cgggcggtgt
    20341 tgccgcgaat gtaaagctta accaacgcct tcacgagata ccggaggttg agcagttgtt
    20401 tgttttgcct aatatgggcg atggaggcct tggactgggc gctttatgtg ctcatgatgc
    20461 agttgccccg catcgcgtgc ccgatgtgtt ctggggtgaa tcctatacag atgccgaaat
    20521 taaatctgtg ctgtcacagt ccggtcttcc ctaccgacgc gtagaggcca ttgaaaacac
    20581 aatcgccgac ctgcttgtca aagaatttct tgttgcgcgt tttaccgggc gtatggaatg
    20641 gggaccccgc gctctgggca accgctcaat tctggcgaaa acgacggaca gcaaagtggt
    20701 cacccggctg aacgctcttc ttcagcgcag tgactttatg cccttcgcac ctgctgtgct
    20761 cgccgaagat ctggacttat actgtctgaa cacaggcgca gggcgccacg ccgctgaatt
    20821 catgactgtc tgcttcgatt gcacggaaaa aatgaaacag gagaaccctg ccattgttca
    20881 tgtggacggc acagcgcgcg ctcaggcagt aagtgccgga gcgaatcctg atttttataa
    20941 gatcctgaaa gccttcaaag atatttccgg aagcagcgtt cttctcaaca ccagctttaa
    21001 cgtccacgaa gagcccatta ttcgaacacc ggaagaagcg attaccgctt ttaaaaaagc
    21061 acggcttgat tatcttgcaa tcggcccctt cctcgtagac ggctttccca tctgcagata
    21121 atactcagaa gacaccgact aaaaaacctc tgtcacatct gcataaaaaa aggagtttct
    21181 gcttttatct ttggatttat gaagtgtctt tgtgtatggt atgaaatctt cagttacagc
    21241 actggtttca actttttact tttacaggaa ttttggcgtg aacaaatatt tcgctattct
    21301 ttttctcatg cttattgttg cgttctctgc cgatgcgggc gaatcgccac agttccgcgg
    21361 accctggcgg gacgggcgct ttgatgaaag taaacttctt acgacatggc ctgaggaagg
    21421 gccgccctgc ctgtggacaa cacacggact ggggcacggc tattcttctc cggtgatcgc
    21481 tgacggaaaa atttatatac ctggcatgat tgatgaagcc accgcgggaa tttttgtgct
    21541 gaatctctca ggcgaaattg aacgagtcat ccctgtcggt attgaaaccg aagacaccca
    21601 ggcaccagga ccgcggtcga ctccgaccct tgacggggat cgcctttata tgctctcagg
    21661 gcttggtgaa ctcttctgcc tgaatatcgc ttccggagaa aaaatctgga gcgttaatat
    21721 tcttgagcgt ttcaaaggtc cgaatattat gtacacattg gctgaatccc tgctggttga
    21781 cggcgaccgg gtcatttgca cgccgggcgg agcagatgca gggctcgctg ctctcgacaa
    21841 atataccggg gaaactgtat ggacaacgcc gggttttacc gatgaggcct cctattgcgc
    21901 accgattatc tacacacaca agggccggcg cctgctgctt actgaaactg cgaattatct
    21961 tgtctgtctg gatgcgaaaa acggctcttt cctctggaaa atggagcatc ctacccgcga
    22021 cgggattcat gcggtaacgc ctctttatct ggatggtctg atttattata cgggcggcta
    22081 cgaagcggaa agcggggctg ttcgcctttc agatgacggc agcagttacg aaaaagtctg
    22141 gcaggacaac ttgctggact gccagcatca cggtgtagtg ctgcatgaag gttatctgta
    22201 tggaaccagc cacatgcgcg gcaatcatct ggtttgcatc gagatgaaaa gcggcgaaat
    22261 catgtggata gcgaaagatg taaaacaggg tgtcgtcatt tacgccgata atatgctgta
    22321 tatttatgaa ggaccgacac gaggcatcgt ccatctggtg aaagcggatc caacagcgtt
    22381 tcaccacgcc ggctcttttc gaatcacgga aggcgatggc agacactggg cacatcctgc
    22441 tattgccgga ggcatccttt atattcggca cggtgatgcc gtcatggctt atgatcttcg
    22501 ttcaaagcca taagaacgct cagcgttgaa aaacgcatct gcaggctgca tgcggcctct
    22561 gttgctgttt cagagatatt taatataaga ctttgctccg gggtctttct tcctgtctgc
    22621 agggaatatg ctaactgtcg gtattccttg tatctaaaac acgctttttt aaataatgtt
    22681 aggaaatatt gctagtcttt aaagaaagtc tgggaaattc gatgctgttt tcatacaatg
    22741 cttttaagcg catacctttt tattctgctc tgattctttt tgctgtgtcc tggggtgcta
    22801 cggctctgtc ggtagacaat gcccttgaca gtgcacgcag aaggctggct gaaaactatg
    22861 aactcagtca attgcgatcg atgacaccac agcaaattat ggagcagctg actgaggaag
    22921 aatgcgctgc ctttgccaat gagcacatcc gctttcagat caactgcccg gcaaaagttt
    22981 acgtactcta cgacagtcgt tttgaagaga aaggcccctt tgaagctttc tggctgaagg
    23041 accatgcttt tgagcgtact gatgtgcagg tgtccagtga cggcgattat tttgttggct
    23101 acgcccggga ttttgatgcc ggagccgtcg gactggggat caattctttt tcaggaaact
    23161 acctccacta tttcatcgcc gtacagccgc tccggaagga agacaccctc actatttccg
    23221 gaatttaccc tcaaagcatc cggacagcag cattccagaa aggcgtccgc ccttatacgc
    23281 accctgaatg gcagcccatt gacagctatt ctcctgcact ggaaggggca acgctgcttc
    23341 aggtggatgc ccggtggcgg cgcaccacca aactgctcga cttttttgcg gaaacagact
    23401 accccgcctc gccccggccg gacaatataa ttctcacctg gagcagcgat ccccggtata
    23461 cccagagcat cctatggcgt acgagtgagg agtcccggca aggcgctgtt gcttatatga
    23521 aaaagaaaga ctacaggcac ttcacccccg gtgcacctgt tataaaagaa gcgcaatcgc
    23581 tccgccacga atccatacat ctcttgaacc agcccgtcat ctatacccat actgccacgc
    23641 tgacagatct ggaaccggac acttcctatg tttatgcagt cggagacggc tcatcggacg
    23701 gctggtccgc attgaacgaa tttacaacag ctccggataa agaagcccct ttctccttca
    23761 tctatatggg agatgttcag gtcggctatc cccgatggcg tagccttctc gagcgtgcgt
    23821 ggcaggatca gcccaatgcg cgattttaca tgcttgccgg agacaacgta aatcgtggcg
    23881 tggatcgcaa tgaatgggat gcgttttttg cacacacaac gggcgttttt gatcatcgtg
    23941 ttgtggtacc cgctctcgga aaccacgaat atattggcgg cgatccgaca ctttttgaag
    24001 atttttttgt cctgcctgaa aacgggccgg aaaatattgc acccgaacat tgcttttctt
    24061 ttgagtacag caatgctcta tttgttatag tggataccaa tctgctcccg gaggaatacg
    24121 tcggatggct ggatgaaacg ctgggacaaa gtcatgcggt gtggaaattc gtcatgatgc
    24181 accacccgct atacagttca gggatcaacc gggacaacgt ctggctccgg gatacgctgc
    24241 ttcccgtctt tgatcgacac catgtggaca tggtgctgca gggccatgac catgcttatt
    24301 tgcgcacagg tcccatgcgg gacaatcaga aagcggagcg tccggcggac ggaacttatt
    24361 atgttgtttc cgtgtccgga acaaaactgt acaaacttgc cgaccggccc tatgcagacg
    24421 ttgcctatga acacctgtct acctatcagg tcatcgacct gcagctgaat ccgccacggc
    24481 tggtctatcg ctcttttgac gtggacggca atatgaaaga tgaagtcatc atagaaaaat
    24541 aagaatagcg tggcatttcg tttcaggaat caacttcaac taacccggga aatttttcca
    24601 tgagcaaagg tttgtttgcg caaaacatta ttgcagtcat ttgggacttt gataaaacac
    24661 tgtctcccca ctatatgcag acacctcttt ttcagcatta cgacgtggat gaatcacagt
    24721 tctggaaaga agtgcaggca ctccccgctt actataaaaa agcaggcatc aatgtccatc
    24781 aggatacctg ttatctgggg caccttctga cctatgtccg cgcagggatt atgaagggtc
    24841 tgtccaatgc tctgctccgg gaaatgggca aaaaaattga tttttacccg gggattccgg
    24901 atatttttga tcgcctgaaa aaaattctgg actcacagaa atatcaggag attgatctgc
    24961 atctggagca ttacgttgtc agcaccgggc tcgccgaaat gatcaggggt tcggttgttg
    25021 ctgaacacct gagcggcata tgggcaagcg agtttatcga aacacctgcc gcccctgcat
    25081 tcaatcctga cacctcgccg gagcccggag aaatcacgca acttgctgga tgcctggaca
    25141 atacgaccaa aacacgcgcc atttttgaaa ttaacaaagg agtgaataaa aaggaaaata
    25201 tctccgtgaa tgacacaatc ccggagtcag aacggcgcgt gcccatcaaa aatatgatct
    25261 atattgcaga cgggccaagc gatgtaccca gcttttctgt catctgtaaa catggtggaa
    25321 aagcctatgc tgtatacgac acggattcca cggcacgctt ttcccaggtg gatgatctgc
    25381 aacggagcgg ccgcgtggat tcctacgggc cagccgacta cagagaaggt ggacatacgg
    25441 acatgtggct caaaaagtac atcacggcca tcgctgaccg aatcgttgat gaaaggcggc
    25501 aggctttgga gaaacaaatt ggtaaagggc ctcagcatct gtaatcaaaa gcacgataat
    25561 tccacgttct gcaatagaac atttgccaaa aggagctgtc cgctatgaac actctctgcc
    25621 tgcttgcatc ggcactgctc gctattcagt ccgcattcac tatggaagat gacggtacag
    25681 cgctctccct gtatgaaaac ggaaatcccg tgctggttta ccactacggc ctggtcaaac
    25741 ctgcctggcc tgtgccggaa cattatacac ggggctgcta tttccatccg ctatacggcc
    25801 ttgatgggga aatactgacc caggactttc ctatcgacca tttccatcac cgcggccttt
    25861 tctgggcgtg gccggacagc ctgtcgggtg atagaaagat tgatgtatgg attctgaaag
    25921 atgccaggca gcatcatgtg tcctggatca aaaaagagcc tggaacggaa agcgcattct
    25981 ttgcgctggt taatcactgg gcttttgacg gtacgccaga cacagcaatc atggaagaaa
    26041 aagtgacgtt tgaggtagct cccgaaaaag agaaccaccg gttccttgat tttacactga
    26101 cctttacgaa cgtcagtgac gctgttttaa ctttacgggg cgcaggtaca gacaacaaag
    26161 gctatggagg attctgcttc cggcccgatg ccgcccgaaa accttttatt ttcagctcgg
    26221 cacagggtgt ttcagaagaa gatgccctga tgctgaattc tccctgggcg gacatttctt
    26281 tcccggaaaa accgggcgga aatatctatt caggtgttgc tatttttcag catcccggcc
    26341 tgcccgggtt ccctcataca ggatggattc tcaggcatta cggcttttta gggcaatcct
    26401 ggccccatgt ccgggatcat gtgatgcagc cgggcgattc cttcacgtta aaataccgtc
    26461 tggtcatcca tcgcgggact ggcgaggatg caggcatagc gtcactcttt caggaatatc
    26521 tctctgaatg ttcttccacg aacgacaccc gcacccccta ataaatcgga cgccccatga
    26581 aaagttctga cctcgactat aaactgcccg aatcgcttat cgctcaatat ccggcggaga
    26641 aaagagacgc ttcccggctc atgatcatcg atcgcaacag cggtcgtatt cagctggatg
    26701 tttttgaaaa tatgccggac tatctgaatc cgggtgattg ccttgcgctg aatgacacca
    26761 gggtcatacg tgcccggctg cacgggcgaa aagaaacagg cggtgcggta gagatttttc
    26821 ttctgcagga actgagtgac ggcggatggt ctgcactggt tcgcccttct gccaaagtac
    26881 gggcgggtgc aaagattctc ttccctgccg gtctggaagc cgtcgtggct gaaacgctgc
    26941 cgggcgggaa gcgacgtgta ttttttaacg gtgaacaggt tatttcccgg ctggaggagg
    27001 caggagaaat tcctttgccg ccttatatac accgcaaaga gtccgatcat aatgacacaa
    27061 cacgctatca gaccgtgtat tcgaagcatc ccggagcagt tgcggcaccg acggccgggc
    27121 tccatttcac agagaatgtt ttcaggaagc tggccgctgc gggcatcgag aaagcatatc
    27181 ttaccctgca tgtaggctac ggcactttca agcctattca ggcggacagg ctcagtgacc
    27241 atcaggtaga tcaggaagag tacatttttc cggaagaaac ggctcaaaaa ctgaatgaca
    27301 caaggcactg cggacataaa gttgttgccg tagggacgac ctgcacccgt gtgctagaat
    27361 cctgttattc cgatggcaaa tatagcggcg gaaacggaag caccgattta tatatttttc
    27421 caccctacac atttcgggct gtggatgttc tgcagactaa tttccacctg ccgcgctcaa
    27481 gcctgcttgc tctggtttgc gcttttgccg gcagagaact gctgatggac gcctaccatc
    27541 tcgcaattaa agaacagttc cgtttctatt cttacggcga tgtcatgctc attcgctgag
    27601 aaagccccaa acgcctttct ctcttttgat attccggcaa acagtttttt atactgtaaa
    27661 ggaaatggcg ggaaggaggc attttacctt ttttaaaggc actgaccgta tactgcctgt
    27721 tggatcagtt caggagcaag cctaaaaatg acaattccca tggtggattt gcgtgcccag
    27781 tatcacgctc tccgcaaaga gatagaaaaa gcagtgcttt ccgttctgga gcggcaacag
    27841 ttccggggtg gcagcgacgt ggaatccttc gaaaaagcat tggctgattt tgcgggagta
    27901 aaacatgctc tcggggtttc ctccgggaca gatgcgctgc tgcttccgtt aaaaactctg
    27961 gatcttcgtc cgggcgacga agtcatcacg acgcctttca cctttttcgc tacagctggc
    28021 gctatcgtaa atgcaggcgg tatt
//
LOCUS       DAOPWB010000024        27726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1698_length_27726_cov_5.770807, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000024 DAOPWB010000000
VERSION     DAOPWB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 27726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27726
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1698_length_27726_cov_5.770807"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..2406
                     /locus_tag="PKN92_03820"
     CDS             115..2406
                     /locus_tag="PKN92_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47953.1"
                     /translation="MCTSGICKGRAVISLLVAVVLIGCFSASALTDPVPRILFVGDSW
                     TTFMLAFKSFRSVFAEDPELVRWVEVGNRTAEAGGRVWEMLERPYLSIVTEELMRYPN
                     VDVVVITLGGNDLLRGLKGGNPSNPTQKVSIKDCFNRPEFSGDPDACLQNLADELEEQ
                     IGIVVDHILSVRPDIRVAILSYDYAARAPRSGDNFTIEQQHNAFVAADLGKYAVALAR
                     DRVEYVNNYGLMQYIYGVPEAEPPIAPGVAPYPCDTPDPGTCAFWPGGYPQYLAPLES
                     YIDQDIHLTAEGYRHVAQRAVDMFIGEWLSYPKALEIKPLQNKAIYQFDVTFSHPVNG
                     VDLSDFEVFIKDKVGLKAMDVINVVQADAEGIVYTVTVDMDGSEQVAYIRVLDDDSIT
                     RKDTGVALGGPGAGNGFFEYNGLYEFQDMIAAADDDFRGALNYLYMASQAYEHMLGEF
                     GFSFNPDLLDANGDLLKDGSLSDPYVIPGNGMLDLWEFMVIDAILKRPNLDLSATGGV
                     THAAIKHAWEEQIASIQEALGGIGGLADIIFPGMDTMLAGFQMLGDKDSNFLVTTLVM
                     LLGDIEEFPTNMNPGALLSYDPVEPDLEVFPKHWLASNGDADGDGWNNLKEYVYFAPD
                     GLTAYINAVLDPAAQPNLGGGSYEEGDFVRIGMFDRCKYNSTFQWYKDGVLLVDEPGK
                     ITGTNARALNLHSVGPEDAGAYTCEYIQPKTQGVYPSAVYGPIQVTVTEEREMPATGG
                     LGLAILLALGTVGGTAVLRKKQK"
     gene            2657..4243
                     /locus_tag="PKN92_03825"
     CDS             2657..4243
                     /locus_tag="PKN92_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007017407.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNZ47954.1"
                     /translation="MHTEMRLIDLIVLLIYFIAMAGMGPYFARRNKTTENYFLGGRSF
                     PGWLIGLSMFATSISSITFVAYPADAYKTAYLRFMLCLTLPLGIFLASKIFLPFYRRA
                     RVTSAFEYLEVRFGAGTRSYAAVVFLITQVARLGLVLYLVAILVQEMTGLSAEWCILI
                     GGIVTSFYTITGGIEAVIWTDFFQSFLLWFGGFAILAVAVSQIDGGFATVFKVAAEDS
                     KFMLGDLNAAGELVKAPWGFSLTEKTVLMMLVVGLNNWLTEYSSNQNVIQKYVATKNP
                     KEATQAIWICCLCSLPTWFFFMFIGTALYVYFKLNPDPAAQAMLLGTDGARADAIVPY
                     FVVQKLPAGLSGLVIAGVLAAAMSSLSSSINAISAVSIVDIYKRHLAKNRSEKHYMNM
                     ARIISLVASLLMLAFAFMLLSVTEKTLQDTATKITAILGGGLLGLYCLGFLTKRGDGR
                     AVFSGIVFVLAFSTWVTLTEAKLIPKFLPFETYYAGLVGNVLFFAIGYLLAAAFFKPR
                     RDLTNLTIWTQTDDWKALND"
     gene            4309..5193
                     /locus_tag="PKN92_03830"
     CDS             4309..5193
                     /locus_tag="PKN92_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ47955.1"
                     /translation="MKLSRRSFLHTGLCGAGALWLASSRLSQAAVQFKMSACDWSLGV
                     SCDPAGLDLALSLGLDGLEISAADGAADQLKIADPSYRQQYKEAMERTGLSVSSVAMG
                     LLNEYPFATDDRAGAWLEQTIDGTADLGAKVILLAFFSKGDLLQSKGWLSKGGDLKAR
                     EVDAVVERVKAAVPRAEKAGVILGLENTLSAAQNLAILDRIQSDAVKVYYDVGNSTYS
                     GYDIAREIRELDDRICQIHFKDGGFVLGEGKVDMKAVADAMTAIDYQGWIVLETAVQK
                     KDRDASFRQNAAFVRGMF"
     gene            5221..6471
                     /locus_tag="PKN92_03835"
     CDS             5221..6471
                     /locus_tag="PKN92_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47956.1"
                     /translation="MSSSKDNMMKRRSFLQAAATTAAAFTVLPAGAVRGLEANSKLQI
                     GIIGCGMRGPWIGEFFERHADSKVVALHDYFQDRVDEAGVKLSVSSQSRYIGLNGYQA
                     MLADPSVDAVAIESPPYFHPDQAVATLAAGKHLFLAKPIAVDVPGCLAIKDAAARANG
                     KLSAWVDFQTRATEAFQGAAQRLHEGHIGEPVCGQFHYYSMPMPMVCKDDSSAPGRLR
                     NWVFDIALSGDIIVEQNIHVLDVANWYLQGHPIKVRGKGGRKARTYCGDCRDHFLMTY
                     TYPNDVLVEFSSGQFTYGFSDLCMRVFCTLGTVDSHYGGDVSVRGKSFSWDGGRTNTI
                     YEDGAIANVKRFADSIRNGQPINNVDESANSNLASILGRMAADSGEEVTWDEMIAKGE
                     RVDAHLELPEDGPFHVRERPTYAP"
     gene            complement(6516..7418)
                     /locus_tag="PKN92_03840"
     CDS             complement(6516..7418)
                     /locus_tag="PKN92_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNZ47957.1"
                     /translation="MPEGRIMLEFWQAALSQPLAQNAVLAALLVSVACGVVGSYVVVR
                     RITYIAAGVAHCVLGGMGAARYLQTVYGWTAFHPIYGAMIAALGAAAIIGWVSLYSKE
                     REDSAISAMWSMGMAAGVLFIYITPGYTGDLMGYLFGNILLVTRHHLYFLLGLDVIIL
                     LLAVRWYDEFQAVCFDEEFARTRNINAGFFYILLLGLTALTVVALSMIVGIVLVIALL
                     TLPAALSGRFAGSLMQMMAWASGLCALFCFAGLTLSYKPDLPAGPTIILIAGAAYFSA
                     AVFSLIRHKLQHQGAKKKSARPNR"
     gene            complement(7393..8145)
                     /locus_tag="PKN92_03845"
     CDS             complement(7393..8145)
                     /locus_tag="PKN92_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ47958.1"
                     /translation="MSTAIHIDRATVNYGSFCALENVTMQIRQGQLVTIVGPNGGGKS
                     TLFKLILGLVRPVTGSVQVLGMPPGDARLSMGYVPQSSLFDPLFPVHVFDVARMGCLG
                     AGNPGPFRKRKETRERVMNALEAVGLDGLANRWFSSLSGGQRQRTLIARALVSQPEVL
                     LLDEPTSNVDIAAEDMILDILDKLKGKMTILLVTHYPQVAMRFLDHVYCVNRTVHIHP
                     PTQRMDETLMRHITGMSPPNGSTEDAGGQNHA"
     gene            complement(8182..9153)
                     /locus_tag="PKN92_03850"
     CDS             complement(8182..9153)
                     /locus_tag="PKN92_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNZ47959.1"
                     /translation="MNQYLKVTQRFLAVSVIAVLMAGCGGSSQNDGTAAPDSVGSSLP
                     VKVIVSIVPQAWIVDQIGGALVQVEVLAPPHHSPETYQITPRQMDALGKAALYFRIGM
                     PFEGALVDRLHSTFPKLVIADTRNNVPMLTLSAHHHDDADEHEHEHEHEHEHGNEDPH
                     IWLDPLRVKIQAHTVFHALLSKLPEQEAALRQNLQGLENTLDSLHAELSGLLDPYRGR
                     TFFVYHPAFGYFADRYGLLQQAVEFEGKSPGAQRLYALIDDIRQSGATAIFTQPQFNV
                     AELTAVARSAGVEMVTLDNLLYDYPESMRAIGRAIAGGLHRSDSYPG"
     gene            9441..11207
                     /locus_tag="PKN92_03855"
     CDS             9441..11207
                     /locus_tag="PKN92_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HNZ47960.1"
                     /translation="MKRRSIVTVSLSLVVLMSGAAFAAPFALAPVDPAFERWSLEKTL
                     DPGENATGYVPSPVRFHHTPQPSLKGTLPVRFDLRDLNALTAVKHQWQCGACWSFAAC
                     GVLEANLRRATGAVWDFSENHMKNTHGFLLGPCMGGNNYISMAYLSRWTGPLLESDDP
                     YNPSTIVPPLEGAVRQKLLLRGVHFTLDESGAERIKNYIYETGALSATMMWRDADYNS
                     VSRSYYYSGGQGATGHMVMLVGWDDQMVIPGAPGAGAWICKNSWGHYWGDQGFFYISY
                     YDTAFLDDAAGFDTLANPNAYGTLYYYDPFGLTGFAGSGAQLDVYGANVFHAVQEESI
                     VAVGTYAVSDNLSYTVSVIRGGMVNGVPGAPAVSVSGVFETAGYYVVPLPTSVELAEN
                     ETFTVLVRYNSPDFDFPMPLERPIAQFCAATAAPGQSYISEDGLVFTDLPGMGAGWAN
                     SNVCIKAVAAYKEQEVPVPSVHIVGRPKVETGQLAVLHAVTAHLTEPMTFFWYKDGVL
                     LPGAEFRDYTIPSVSAADSGSYVVQVSDDSQGVFNSPPFVLEVLEEGSLPVAGAPALL
                     LLVLLLTLAGAFLFLNRLSSIK"
     gene            11279..12211
                     /locus_tag="PKN92_03860"
     CDS             11279..12211
                     /locus_tag="PKN92_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47961.1"
                     /translation="MNRSGFLRTVFVLLAVFCLLHGGQLQAEERILLIGDSWAQGIWM
                     AGLLDKALAEAGFPEMTAIGESCALGGTRADQWNKPEYREKILDALALSPTVDMIHLI
                     IGGNDVLKRIRDTNVFTAWSEKKRDKEWDLIAADIRDLVEFCLSIEQVKCVGLAGYDY
                     LNASTAKEALGMLGQNFDFGGMSQEQVNACMIALEKRKKDLAASIKGCVYIHNFGLLQ
                     HHFNDPEGTPLPGAPPEYVSFPGGDPARPMPDAAFTKVSFGGREFSGDGIHPGEEAHM
                     VMLRNGMQCCYVPYLRSLTEKQATAEHDDRSGGN"
     gene            12186..14201
                     /locus_tag="PKN92_03865"
     CDS             12186..14201
                     /locus_tag="PKN92_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ47962.1"
                     /translation="MMTDPGGTDVAALWLSSLEWTLSGWRPFAWKLGKTAPEPNPLCP
                     DILPVPVRFPMSVQQALLEAGIIPDWNKGMNSLLCEWVEHRHWEYSSRLRLSPSEYGD
                     KVFLDAPGLDYSGWILIDGEEVVRFSSTMSAHRIDLGAALLDGTEHELSIIFEEPPRE
                     QGQMGYTSRSRFFKSRYNYGWDWCPRIVPIGPWLDACIPDRLESRKPDFYVSARLEED
                     FRHGTVEVRLMLPDAAEERSFRLSLSDGTALLGEKSFCLKRGGAQKIQLTRLPVEAWW
                     PNGFSGQKRYTLTIEEIDGSGTVLWAGHRRAGFKHIDWLPCEGAPDGADPWLCCVNGA
                     PIFLQGANWVPPRAVYHDAQREHYATLIRLYKDMGVNLLRVWGGGILEKEVFYDLCDE
                     AGILVWQEMPLSSSGIENCPPEDSEAIQTLQKIASNYVRLRGHHVSLLLWSGGNELTW
                     GSPDNPFGETPVDMRHPAIHALKVQLEQLDPGRRFVATSPTGPQFYAHPENYGKGLHH
                     DIHGPWGLSRFERADFGDSLDVWKQYWAEDDSLFRSEAGMPGAASLECLEQFAPLGES
                     WPFSEGYWRHTASWWTQERLYRAVVQDGIDTAARYVAVTQDAQAQAYAIAAKACKSRF
                     PQCGGFVVWMGHDCFPCPANNSLIDYLCTPKAAYKSLQAIFLAPPGA"
     gene            14413..14841
                     /locus_tag="PKN92_03870"
     CDS             14413..14841
                     /locus_tag="PKN92_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47963.1"
                     /translation="MKVLLKGMFVGLFVLALTAGCASVGSKVSDQDLIKDLVNQLKVA
                     LENKDIDGIMNTFSDDFYHPEVGGKEEGKEMLQMALDAGYADNGEVFVDDMEIELKDD
                     GTATVYPIDLSADPGSISIELLVKKEAAGWRILEVRPDGM"
     gene            complement(14941..15921)
                     /locus_tag="PKN92_03875"
     CDS             complement(14941..15921)
                     /locus_tag="PKN92_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47964.1"
                     /translation="MTLRLYMLLAGLILWGTLAAVHPVNAGADDEMEAHYGTFTLPGF
                     QCTIGDNTAADPHMRGYNGIFSLLPSGDGQNVFVPLFSGLNLEHYFDALPVTEPGSVF
                     FEPRRAAMQFTRIDEHTAELYQPVTPVFGVESRTRFTATAPHYIDMHFSCTPHRSDLA
                     GGLLGIFWASYMNAPEDKSIYFLGDGSSLESPRWIQFCTQEHNRDSTVCSENDRVNIA
                     FQGTEKTLFTAISPLRYAVPFFYGRIREQVLIYIFQPGPLIRFAHSPSGAGATEKGDD
                     TNPAWDFQMIIPDCEPGTSYTLDMRLVCKKWQGRDDVLEEVRRFLSGTDK"
     gene            complement(15950..17038)
                     /locus_tag="PKN92_03880"
     CDS             complement(15950..17038)
                     /locus_tag="PKN92_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNZ47965.1"
                     /translation="MNLGALSMTRRHFLGLSTAALAGTALYAPAESLLHSGKQAPLPL
                     WHGFNLLEKFMMPHCGAFEEKDFAWIQETGFNFVRLPMDYRHWTDPDDWTELKEEVLR
                     EIDEAVQFGERYNIHVCINFHRAPGYTVAQPGEEKNLWDDREALETCALHWRHFARRY
                     AGIPNDRLSFNLLNEPALVTAKKHYAVIEALVQAIRNEDASRLIICDGRLYGKVPPDE
                     LMPLGIAASTRGYEPFELTHYHASWVQGADQFPLPSYPLRKKETLMDKSWLKEKQIAP
                     WKAAEEKGMPVMVGEFGAFNQTPHSVTLAWMQDCLELWKEAGWGWALWNFRGAFGILD
                     SGRSDVAYEAWNGHQLDRAMLSLLQRYL"
     gene            17291..18223
                     /locus_tag="PKN92_03885"
     CDS             17291..18223
                     /locus_tag="PKN92_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HNZ47966.1"
                     /translation="MRKKSIVAPDWWDYTTLDDELLNDVANLTEKDLFQLSRDGFKVV
                     FHDSLESFYLAEALQYIEAWKSSTPDNPAGVCGPIGPTEQLPLVAMLVNELNINLSKL
                     GAQFWGMDEWVKDGKTLPAEHPLSFQKANLDLCFNLMRPELRPRPEAIHYPIGDFAAF
                     SKSFDDMRCLVMQGGQGDVKHWAFNDPMKREGVWADAPPSPEEYLKLGTRVVDLHPMT
                     IMQNARTSGGGRLSEIPTQATTVGPAETWKAEKVSIWQAGNHDNPFGMRLTAFMISKG
                     LADSAVPMSLLALHPNVEFHYYRKAIGSCGIEMH"
     gene            18235..18999
                     /locus_tag="PKN92_03890"
     CDS             18235..18999
                     /locus_tag="PKN92_03890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNZ47967.1"
                     /translation="MRLVDVHCHLDSPAFERRLHDLLETARSAGVCAWISAAIHPSGW
                     EAVRMLSRCHAGVYHALGVHPWYCSDDSLPLIPSLASQASEAVAIGEIGLDSKRPVFP
                     LERQLSVFSRQLGIARELKLPVILHCRGAFNEMLQLFRKEGAPAGGILHNFSGGPELA
                     KAFMDYGLAFSLGGVLTWRNSARRTLLLKEIYPHSLLLETDAPDIPPVEARGTVNSPA
                     NILYNLKAASELLDVPAEEIAAFSTANAGRIFGIAL"
     gene            19011..19844
                     /locus_tag="PKN92_03895"
     CDS             19011..19844
                     /locus_tag="PKN92_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ47968.1"
                     /translation="MTILTLLVTLAAAAGQLADPFPGPPRHGLVYVIAHRGVHDGIPE
                     NTVAAYKKAIELGVDYVEIDVRETKDGQLVSVHNATVDAYTEDAKGPVKDFTLAELQA
                     MDIGSRVGDEWKDERIPTVDAILEACSGNVSIYLDFKQAPPEEVVDKLRAKGLAESTV
                     WYAGGGPLRALNKVCPECHPMPEAGSGENLDRLLQLHRPRVVASTWGAATREMVDRCH
                     AEGVLVFVDDGGPDTWQTLFEWGVDGIQTDHAAELIEAVKAYTASAKKEEDSGGVAGA
                     P"
     gene            19841..20719
                     /locus_tag="PKN92_03900"
     CDS             19841..20719
                     /locus_tag="PKN92_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ47969.1"
                     /translation="MKPRLSVVVPSYLPDPVLLRKLYDSLQAQDFTDFELLIVDDGSP
                     DLSAYDFPEDARVRLIRQEKNAGPAAARNRGAAEARSDHLFFTDTDCELAGGTLAAAS
                     AALQKDDIFSGNTRTAVKTRIGRAIALLGFPGGGILGFDRVWRVDKDGYTRSFSSCNL
                     AVTRERFFALGGFDESFPLPGGEDTVLAAQACAAGFRIRYVPEMLVYHVEKSTLGSFF
                     RWQLIRGRGNWHIKKRLPSVGGYLRLRVWTFSNSLKAAGILYAVPVLLLIVISVVLQV
                     TGMILEKRRMKKDGSP"
     gene            20782..21891
                     /gene="amrB"
                     /locus_tag="PKN92_03905"
     CDS             20782..21891
                     /gene="amrB"
                     /locus_tag="PKN92_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNZ47970.1"
                     /translation="MQKNVPIVLVLVLFLLGGAASGAPARETPLIRWPAVAGVNYSSD
                     PETLLSTLVNFRMQADVPEINNRLVAIIASPGPYGLAGKTTAHAFKSLQPGQYGRVVI
                     LAPGHGPGFENCSIPAVDIFLTPLGPVPLDGAAIRTLMYSPLFQMHQIRYDGAKPHER
                     LHEYEFAIESMLPYLQERLLEFKLVPVLVGDLRDPQGKTTENTMAAFANAIKPIVDER
                     TLIVVASSFTHYGEEYKNTPFTENIEDNIAHLDRQAFEEILSLNLTGFRAYLKRSKNV
                     IDGQDCIQILMQMLPPHTQARLLAYTTSGAVSGNWKSSVSYGSFVFHDLDAPALSARP
                     DQVRPLILRKPEKTAASAGEAPPAGGQGKTDTAVP"
     gene            21906..22496
                     /gene="amrA"
                     /locus_tag="PKN92_03910"
     CDS             21906..22496
                     /gene="amrA"
                     /locus_tag="PKN92_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNZ47971.1"
                     /translation="MEEKEIQLTPDEKSLLLRIARESVQAYLEEGVPPAMDTYPLTPL
                     LKQKMGVFVTLRCAGALRGCIGHIFAQEGLAETVAVNAVNAGFYDSRFAPLRPEELEA
                     LRFEVSVLLPAEEESPFIPVNDPGEICIGRDGLYLEHPEGRGLLLPQVPVEQGWNGTA
                     FLEGICRKAGVHAGGWQDPGARLWRFRACVFGESED"
     gene            complement(22509..23351)
                     /locus_tag="PKN92_03915"
     CDS             complement(22509..23351)
                     /locus_tag="PKN92_03915"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNZ47972.1"
                     /translation="MFQEGWFEVAPGIKLFERHLLTPSPPRARVIVLHGYGDHCARYS
                     WVMEQFQNTGMATYAYDQRGHGRSPGRRGYVARFDELPDDLDIFLKRVTEDSDPSAPL
                     FVFGHSMGGMVLARWAATRQPDVRGLIFSSPFLAFSDEVPAALIALTPYVAALAPWMP
                     VSQVDNSGLSRDPEVQRRTDADPLSYHGKVAAHTAREFHRTIRIIEKEMPRITVPMLV
                     IHGCCDRIVGPSGSKALFEKAGAADKELKLFEDAYHEIYNDLDKERYMQTVIDWISAR
                     LQSV"
     gene            complement(23364..26222)
                     /locus_tag="PKN92_03920"
     CDS             complement(23364..26222)
                     /locus_tag="PKN92_03920"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47973.1"
                     /translation="MAHPLLKKIFTFFKRLAIFAVIFLTFVLLLPNDFKRLHIHIQGK
                     GSVLPPVKTLHVGRDYHANLLARPLPGTGLGFREWAGTISSTSPEITVEMKGRERSIT
                     AIFSDTPPPGVSFQKVTIQCAGPGSGTTDPAPGSWVFRNDWPEAFTALPDKGSFFGGW
                     EILPEDTGAGESTSARREPFPTCTLPTERNLTAIAWFYRSGALITLETEGQGWYGVPV
                     GTYPLAAGTEVTLRAAAEEGWHLSRIEDEKGQIVLASDNPRWADFSFRVNENKTYRAV
                     FEPLEKRIQVCVDSPPPTAGLVFSGASPCPPVLHALYGSTLTLEARTLNHESAFAGWS
                     GTAHDGISHDALLTLPVTADASLTAHFVPAQCFLDLEAEIDGEPAMLPAGALKPMPGL
                     YGFTGQEKDIQSLEACWISDLDIAFLRWEGDLPEGADRTQRLLHLPMTASRKVRACFT
                     RHNLVSLSVEKEKGSPGTLTPAPGHYRIPAGTQLTLQAVPAAERFFGGWKITRESGEE
                     QVAITPSLTVTAEEGMHASAVFGRGACSLHLASTHPEAEIIPAPGDYTVLVGTPADFI
                     AKPPEGLIFHYWRSFKKGILSENPLLRVVLEEDDGITAVFGLPLYSLQLTAEGKGHID
                     RDWQGIEQFEEGTYVQLHATPDPDAVFLCWEGDLPANALPAQPKILVLMDQDKALTAR
                     FAPADWQLDIRLDLPDGMHAPKLFPPPGLSGRTNGEAVPLMAIPPANSNLVFIGWEGD
                     LLSPDPVRNLIMDGDKVITARFAPAEETEHVRLTVLRTEGSSLSVIRDFDPGLYHFAK
                     NAQIPLTAILSDDAYFGGWTGDVDQDAVYTDKMLCLDRDKTVGLQTGSRGAVLTVFLD
                     GNGAGQISPPPGVYRLAENMTITLQAIRVNTEYHFDGWYTGAGELLSRMGKYRFTIQP
                     GISRMEITGVFRDYVLPPPLVLCWQNNRCASAAADTAL"
     gene            complement(26345..26851)
                     /locus_tag="PKN92_03925"
     CDS             complement(26345..26851)
                     /locus_tag="PKN92_03925"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNZ47974.1"
                     /translation="MTETCTNFCFRFVGGAFCGFFSEAGLKRLSLVPDEKQMPAAVDK
                     ARKASWDKTLHRLLADYFSATPVQFDAIPLDMSGGTPFQQKVWREAYRIPYGGTSTYG
                     TLAQRIGHPGAARATGSALGVNPICLVVPCHRVLSASGTLGGYYYGLPWKERFLALEA
                     SGLAASQK"
     gene            complement(26873..27229)
                     /gene="rnpB"
                     /locus_tag="PKN92_03930"
     ncRNA           complement(26873..27229)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKN92_03930"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(27432..>27726)
                     /locus_tag="PKN92_03935"
     CDS             complement(27432..>27726)
                     /locus_tag="PKN92_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HNZ47975.1"
                     /translation="QPDDPRRQALVARRCMTADEMAVWVKEISTNDRQEEPSIRSLES
                     IYSGYSANAALVLIKVASVLLDRQMASQAAAFEQEGGFSERLYRTRQTGRKKA"
BASE COUNT         5913 a         7134 c         8146 g         6533 t
ORIGIN      
        1 tgaaaaagta tgtgttatca tagacggtgc attacgttca ttatagcagg tgcataagtg
       61 gttggatgtc acaccaccga catgcgcttc aggtattaaa aggagtgttt tcatatgtgt
      121 acatctggta tctgtaaagg gagagctgtg atttccctgc ttgttgcggt tgttctgatc
      181 ggctgctttt ccgcttccgc tttgacggat cctgtaccga ggattctttt tgtaggcgac
      241 agctggacga ccttcatgct ggcgttcaaa tccttccgct cggtctttgc tgaggatccc
      301 gaacttgtcc gctgggttga agttggcaac cgtacggcgg aagcgggcgg tcgtgtctgg
      361 gaaatgctcg aacgtccgta tctttccatt gtgacggagg agctgatgcg ttaccccaat
      421 gtggacgtgg tagtaatcac gctgggcggc aacgatcttc tgcggggact caaaggcggc
      481 aatccgagca atcccactca gaaagtgagc attaaagact gttttaatcg accggagttt
      541 tccggcgacc ccgatgcctg ccttcagaat ctggctgatg aactggaaga gcagatcggc
      601 attgtggtgg atcatattct gtcggttcgc ccggatatcc gtgtggcgat cctcagttac
      661 gactacgcag cccgtgcacc ccgttcgggc gacaacttca cgatcgaaca gcagcacaat
      721 gcctttgttg ccgccgatct gggaaaatat gcggtagccc ttgcccgtga ccgggtcgaa
      781 tatgtcaata actacggtct tatgcagtat atctatggtg ttccggaagc ggagccgccc
      841 attgcgcccg gcgttgcacc ttatccttgt gatacaccgg atcccggcac ctgcgccttc
      901 tggcccggcg gctatcccca gtatcttgcg ccactggaaa gctacattga tcaggacatc
      961 cacctgactg ccgaaggcta taggcatgtg gcacagcgtg ctgtggatat gtttatcggc
     1021 gaatggctga gctatcccaa agcgctggaa atcaagccgc tccagaataa agcaatctat
     1081 cagtttgacg tgaccttctc tcatccggtg aacggtgtgg atctgagcga ttttgaagtc
     1141 tttatcaagg acaaggtcgg tctcaaggca atggatgtca ttaacgtggt gcaggccgat
     1201 gccgaaggga ttgtctacac ggtgacggtc gacatggacg gcagcgaaca ggtcgcttat
     1261 attcgcgtgc tggatgatga ctcgattacc cgtaaggata cgggtgttgc gctgggcggt
     1321 cccggtgccg gcaacggctt cttcgaatac aacggacttt atgaattcca ggatatgatt
     1381 gcagcagccg atgacgattt cagaggcgcc ttgaattatc tttatatggc gtctcaggct
     1441 tatgagcata tgctgggtga attcggcttc agttttaatc cagatcttct cgatgcgaac
     1501 ggcgatctcc tgaaagatgg aagtctcagt gatccttatg tgatcccggg caatggcatg
     1561 ctggatctct gggaattcat ggttattgat gccattttga aacgcccgaa ccttgatctc
     1621 agcgctacgg gcggtgtaac ccatgcggct attaagcatg cctgggagga gcagattgct
     1681 tccattcagg aggcattggg cggcatcgga ggtctggcgg atattatctt ccccggaatg
     1741 gataccatgc ttgccggttt ccagatgctt ggtgataaag attccaactt tctggttaca
     1801 acattagtga tgcttctggg agatattgaa gaattcccca ccaatatgaa tcccggcgcg
     1861 ttgctgtctt atgatcctgt cgagcccgat ctggaagtct ttcccaagca ttggcttgct
     1921 tccaacggcg atgctgatgg ggacggctgg aacaacctga aagaatatgt ctatttcgca
     1981 ccggacggat taactgctta tatcaatgca gttctggatc ctgctgcgca gcctaatctg
     2041 ggcggaggct cctatgagga aggagacttt gtgcgcatcg gcatgtttga ccgctgcaaa
     2101 tataattcta ctttccagtg gtataaagac ggtgtgcttc ttgttgacga gcccggaaaa
     2161 ataacgggca ccaacgcaag agcgctcaat cttcattccg tgggtcctga agatgcgggc
     2221 gcatatacct gcgaatatat tcagccgaaa acgcaaggcg tctatccttc tgccgtctac
     2281 ggtcccatcc aggtgaccgt taccgaggaa cgcgagatgc ctgctaccgg cggtcttggt
     2341 ctggcgatcc ttctggcact tggtacggtc ggcggtactg ccgttctgcg caaaaagcag
     2401 aaataggctg ccgggctgca tttgaaatcc ggccgccctg ttcattgagc ggggcggctt
     2461 ttttatttcc ggaaagcgct ttaaaaagcg ttgcgtcggg ctttggagcc ttgttcttta
     2521 gtatgataga ctttccggac tgaattaagg cggtgatttt tcgaaagaac gccgaagttt
     2581 tgtccttatt ctcttacatg tagttccgga catcttcgta acgtccgcga acaatggtat
     2641 gaaccaagga gtggttatgc atacagaaat gcgtttgata gacttgattg tacttttgat
     2701 ttattttatt gccatggcag ggatgggtcc ctattttgcc cggcgcaaca agacgacaga
     2761 gaattatttt ctgggcggca gatcctttcc cggctggctt atcggtctgt ccatgtttgc
     2821 cacgtccatc agttccatta cctttgtggc gtatccggca gacgcttata aaacggcata
     2881 ccttcgtttt atgctgtgtc tgaccctgcc tttgggaatt tttctggcct cgaagatttt
     2941 ccttcccttt taccggcgcg cccgggtaac ctccgctttt gaatatctgg aggttcgttt
     3001 cggtgcgggc acacgttcct atgcggccgt tgtgtttctg atcacgcagg ttgcccggct
     3061 gggcctggtg ctgtatctgg ttgccattct tgtgcaggag atgaccggtc tgagtgcgga
     3121 gtggtgcatt ctcatcgggg gtattgttac ttccttttat acgatcactg gtggtattga
     3181 agcggtgatc tggacagatt ttttccagtc ttttctcctg tggtttggcg gcttcgcaat
     3241 tcttgcagtc gccgtatctc aaatcgatgg cggttttgcc accgttttca aggtggcggc
     3301 tgaagacagc aagttcatgc tgggtgatct gaatgcggcg ggtgaactgg tgaaagcgcc
     3361 ctggggcttc agtctcacag aaaagacggt gctcatgatg cttgtggtcg gtttgaataa
     3421 ctggctgaca gagtacagca gcaatcagaa cgtgattcag aaatatgtgg caacgaagaa
     3481 tcccaaagaa gcgacacagg ccatctggat ctgctgcctg tgcagtctgc cgacctggtt
     3541 ctttttcatg ttcatcggca ctgcgctcta tgtgtacttt aaactgaatc cggatccggc
     3601 agcgcaggcc atgttgttgg gcacagacgg tgcgcgggcc gatgccatcg ttccttattt
     3661 tgtggtgcag aaacttcctg cagggctttc cggtctcgtc attgcaggag tgctcgccgc
     3721 tgccatgtcc tccctttcgt caagtatcaa tgcgatttca gcggtgagca ttgtcgatat
     3781 ctacaaacgg catctggcga aaaaccgcag tgaaaaacac tacatgaaca tggcacgcat
     3841 tatttcgctg gttgcctcgc tgctgatgct tgcctttgct ttcatgctgc tgagtgtgac
     3901 ggaaaagacg ctgcaggata cggcaacaaa gatcaccgcc attctcggcg gcggtctgtt
     3961 gggtctgtac tgcctcgggt tcctgacgaa gcgcggcgac ggcagagcgg tcttttccgg
     4021 aatcgttttt gtacttgctt tcagcacctg ggtgaccctg actgaggcaa aactaatccc
     4081 caaattcctg cccttcgaaa cgtactatgc cgggcttgta ggcaacgttc ttttctttgc
     4141 aatcggctat ctgctggcag cggcattttt caagccccgc cgtgatctga caaatctcac
     4201 catctggact cagacagatg actggaaagc gttgaatgat taaaaggctt tgcgaacgtg
     4261 cggcagcgcg tccggaatga agcatgtgct tacggaggag acttcataat gaaactttca
     4321 cgtcgttcgt ttctgcatac gggtctttgt ggcgcaggtg ccctgtggct tgcctcgtcc
     4381 cgcctgtctc aggcagccgt acagttcaag atgagcgcct gcgactggtc tctgggcgtg
     4441 agctgcgatc cggccgggct ggatctggcg ttatcgctgg gtctggacgg gctggagatt
     4501 tcggcggctg acggtgcggc ggatcagttg aaaattgcgg atccttccta ccggcagcag
     4561 tacaaagagg ctatggaacg gacagggctt tccgtatctt ctgtggcgat gggtcttttg
     4621 aatgagtatc cttttgccac agatgacagg gcaggagcct ggctggaaca gacaatagac
     4681 ggcaccgctg atctgggcgc aaaagtgatt ctcctggcct ttttcagcaa gggcgatttg
     4741 ctgcagtcca aaggctggct cagcaagggc ggtgatctga aggcaagaga agtggatgcc
     4801 gttgtggagc gggtgaaagc tgcggtgccc cgggcggaga aagccggcgt catcctgggg
     4861 ctggagaaca cgctttcagc ggcgcagaac cttgccattc tcgaccgtat tcagagcgat
     4921 gcggtgaagg tgtattacga tgtggggaat tccacctaca gcgggtatga tattgcccgg
     4981 gaaatccggg agctggacga ccggatctgc cagattcatt tcaaagacgg cggttttgtg
     5041 ctgggcgaag gtaaagtgga tatgaaagcc gttgcggatg ctatgacggc gattgattat
     5101 cagggctgga tcgttctgga gactgctgtt cagaagaagg atcgggacgc cagtttccgc
     5161 cagaatgcgg catttgtacg aggcatgttt tagataaaat ttttcacaag gagcgggatt
     5221 atgtcatctt cgaaggacaa catgatgaaa cgccgaagct ttcttcaggc ggcagcaaca
     5281 acagcagcgg cttttaccgt gcttccggca ggggcggtgc gcgggcttga agcgaacagc
     5341 aaactgcaga tcgggattat cgggtgcggt atgcgcggtc cctggatcgg cgagtttttt
     5401 gaaaggcatg ctgattccaa agttgtggcg ctgcacgact attttcagga tcgcgttgat
     5461 gaggccgggg tgaagctgag cgtatcttcc cagagccggt acatcggatt gaacggctat
     5521 caggcaatgc tggccgatcc ctccgttgat gccgttgcga ttgaaagccc gccctatttt
     5581 cacccggatc aggctgtggc aacgctggca gccgggaagc atctctttct tgcgaagccc
     5641 attgccgtcg acgtgcccgg atgcctcgcc atcaaggatg ccgccgcccg cgccaatggc
     5701 aaattgagtg cctgggtcga tttccagacc cgtgcaacag aggctttcca gggtgccgcc
     5761 cagcgactcc acgaaggaca tatcggcgag ccggtctgcg ggcagttcca ctattacagt
     5821 atgcctatgc ccatggtctg taaagacgac agctcggcgc ccggccgcct gcgcaactgg
     5881 gtctttgata ttgcgctgtc gggcgatatt attgtggaac agaacatcca cgttctggac
     5941 gtcgccaact ggtatctgca ggggcatccg atcaaagtgc gtggcaaggg cggcagaaaa
     6001 gcacgcacgt actgcggcga ctgccgggat catttcctga tgacatacac ctatcccaat
     6061 gatgtactgg tcgagttcag ctcgggtcag ttcacctatg gcttcagtga tctgtgcatg
     6121 cgtgttttct gcacactggg tactgttgac tcccattacg gcggagacgt gagcgtccga
     6181 ggcaagagtt tcagctggga cggcggaagg accaatacca tttatgagga tggtgctatt
     6241 gccaacgtga agcggtttgc ggacagtatc cgaaacggcc agccgatcaa caacgtggat
     6301 gaatccgcca acagcaatct ggcgagcatc ctcgggcgca tggctgccga ttcaggcgaa
     6361 gaggtgacct gggacgaaat gattgcaaaa ggagaacggg tggatgcgca tctggagctt
     6421 ccggaagacg ggcctttcca tgtgcgtgaa cgaccgacgt atgcaccctg atcgctgctg
     6481 acggacaaat aacatgctgc ccggtcgtcc aggaattaac ggttcggccg ggcacttttt
     6541 ttctttgcgc cctgatgctg tagtttatgg cggatcagag aaaaaacggc tgccgaaaaa
     6601 taagccgccc ctgcaatgag gataatggtg gggcctgcag gaagatcggg cttgtaactg
     6661 agtgtgagtc cggcaaagca gaagagggcg cacagacctg atgcccacgc catcatctgc
     6721 atgagggatc cggcaaaccg gccggaaagc gcggcgggca gcgtaagcag agcaatgacc
     6781 agaacaatgc ccacaatcat ggacagggcg actacggtga gtgctgtgag gccgaggagg
     6841 aggatgtaga aaaatccggc attgatgttt cgggtgcggg cgaattcctc atcaaaacaa
     6901 accgcctgaa actcgtcgta ccaccgcacc gcaagcagca ggataatgac atcaagtccc
     6961 agaagaaaat agaggtggtg ccgggtgacc agcaggatgt ttccaaagag gtagcccatg
     7021 agatcgcccg tatagccggg cgtaatatag atgaaaagca cacccgccgc catgcccatg
     7081 gaccacatgg cgctgatggc ggagtcttcc cgttctttac tgtaaagaga tacccagccg
     7141 ataatggcgg cagcgcccag tgcggcaatc atggcgccgt aaatggggtg aaatgctgtc
     7201 cagccgtata cggtctgcag ataacgggcg gcgcccatgc cgccgagcac acagtgggca
     7261 acgccggcag cgatgtaggt gatgcggcgc acgaccacat agctgcccac cacgccgcaa
     7321 gccacgctga caagaagtgc agccagcacg gcgttctgtg ccagtggctg agaaagtgcc
     7381 gcctgccaga actcaagcat gattctgccc tccggcatct tcagtgctcc cgttcggcgg
     7441 tgacatgccc gtgatatggc gcatcagtgt ctcatccatc cgctgcgtgg gcgggtgaat
     7501 atggacggtg cggttcacgc agtacacatg gtcgagaaag cgcatggcga cctgcgggta
     7561 atgggtgacg agcaggatgg tcatcttgcc tttaagcttg tcgagaatat ccagaatcat
     7621 atcttccgca gcgatatcca cattcgaagt cggttcgtcg aggagcagca cttcaggctg
     7681 tgagaccagt gcccgggcga tgagcgtgcg ctgacgctgg ccgccggaca ggctgctgaa
     7741 ccagcgattt gcgagtccat ccagtcccac cgcttccagc gcgttcatga cccgttcccg
     7801 tgtttctttc ctcttccgga aaggtcccgg gttgcctgca ccgagacagc ccatccgggc
     7861 gacatcgaaa acgtggacgg gaaagagggg atcaaaaaga gagctctggg gcacatagcc
     7921 catggaaagg cgcgcatccc ccggcggcat gccgagaacc tgcacagaac ctgtcaccgg
     7981 gcgtaccagt ccgaggatca gtttaaagag cgtgcttttg ccgccgccgt tgggaccgac
     8041 tatggtgacc agctgcccct gccggatctg catggttacg ttttccagag cgcagaaaga
     8101 accgtagttt acggtggcgc ggtctatgtg gatggctgtg ctcatggcgc tattatggct
     8161 ttcattgccg ttatctgtca atcagcccgg gtaagaatcg ctccggtgca gtcccccggc
     8221 gatggcacgg ccaatagccc gcatcgattc aggataatcg tacagaagat tatccagagt
     8281 gaccatttca acgcctgcgc tgcgggcaac ggcggtcagt tccgccacat taaactgggg
     8341 ctgggtgaag atagcggttg cgccgctctg cctgatatca tcgataagcg catagagtcg
     8401 ctgggcaccg ggacttttgc cttcaaactc gaccgcctgc tggagtaatc cgtaacggtc
     8461 ggcaaaatat ccgaaagcag ggtgatacac aaaaaaagtt ctgccccgat aaggatcgag
     8521 caggccggac agctcggcat gcaggctgtc gagggtattt tcaagcccct gaagattttg
     8581 acgcagcgcg gcttcctgtt ccggaagttt agaaagcagc gcatgaaaaa ctgtgtgtgc
     8641 ctgaattttc acacggagcg gatcaagcca gatgtgggga tcctcattgc cgtgctcatg
     8701 ctcatgctcg tgttcgtgtt cgtgttcgtc cgcgtcgtca tgatgatgag cactgagtgt
     8761 cagcatcggt acgttgtttc gggtatccgc aatcaccagt ttcggaaacg tgctgtgcag
     8821 tctgtccacc aaagcccctt caaaaggcat gccgatgcgg aagtagagtg ccgccttgcc
     8881 cagggcgtcc atctgacggg gcgtaatctg gtaggtttcc ggcgaatgat gaggaggtgc
     8941 cagcacttcc acctgaacca gtgcgccacc gatctggtca acaatccacg cctgaggcac
     9001 gatgcttaca atcaccttga caggcaggct gctcccgacg gaatccggtg cggccgttcc
     9061 gtcgttttgg ctggaaccac cgcatccggc catcagtact gctatgaccg aaactgccaa
     9121 aaaacgctgt gtcactttca gatattgatt catacagtca cgatatccgg tttttgtatg
     9181 ttttatcctg tcgcttcgcc ctcactcctc tccgagagca atatacacct ttcttccaac
     9241 agatgtaaac acttagttct gcagatttca ttccattttt tacaaaaaga ggacagtact
     9301 aaaaaaaaga actgcagcgc gcgccagtaa ggttctgcga ccaaaaacga aatctgtgat
     9361 acaattttac tactacccgc cgagggagac acaggtgtct acccggcgca gcacaagatc
     9421 atcttccagg agtgcttacc atgaagagaa ggtctattgt cacagtttcc ctcagccttg
     9481 tcgtattgat gtcaggcgcc gcctttgccg ctccttttgc tctggctcct gttgatccag
     9541 cctttgagag gtggagcctg gagaaaacac tggatccggg agaaaacgcc acaggttatg
     9601 tgccgtcacc cgttcgcttt catcataccc cgcagccttc cctgaaaggt actcttcctg
     9661 tccgctttga tcttcgggat ctgaatgccc ttacagcggt caagcatcag tggcagtgcg
     9721 gcgcctgctg gagctttgcc gcctgcggtg tgctggaggc gaatctgaga cgggcaacag
     9781 gtgctgtatg ggatttttcc gagaatcata tgaagaatac ccacggtttt ctgctcggtc
     9841 cctgcatggg cggcaacaac tatatcagca tggcgtatct gagccgctgg acggggcctc
     9901 tgctggaaag cgacgatccg tataaccctt caacgattgt gccgccgctg gaaggtgctg
     9961 tccgccagaa attgctgctg cggggcgtac attttacgct ggatgaaagc ggtgcggaac
    10021 ggattaaaaa ttacatatac gagacaggag cgctttccgc caccatgatg tggcgggacg
    10081 ctgattataa cagtgtttcc cgcagctatt attattcggg cgggcagggt gccacgggcc
    10141 acatggtgat gcttgtgggc tgggatgatc agatggtgat tcccggcgcg cccggcgcag
    10201 gtgcctggat ctgcaaaaac agctggggtc attactgggg tgatcaggga tttttctaca
    10261 tctcctatta tgacactgct tttcttgacg atgctgccgg tttcgatacc ctggcaaacc
    10321 ccaatgccta tggcacccta tattactatg atcccttcgg attgaccggc tttgcaggtt
    10381 cgggggcgca gctggatgtt tacggggcaa atgtctttca tgccgttcag gaagagtcca
    10441 ttgtggcggt gggcacctat gcggtgagcg acaatctttc ctatacggtc agcgtgatac
    10501 gcggaggtat ggtgaatggt gtgccgggcg cgcctgcggt ctctgtcagc ggggtttttg
    10561 aaacagcagg ctattatgtt gtgcccttgc cgaccagtgt cgaactggca gagaatgaaa
    10621 cgtttaccgt gctggttcgg tacaattcgc cggattttga ctttcccatg cctctggagc
    10681 ggcctatcgc ccagttctgc gcagccacag cggcgcccgg tcaaagttat atcagtgaag
    10741 acgggcttgt ttttacggac ttgcccggta tgggcgccgg atgggcgaac agcaatgttt
    10801 gcattaaagc ggtggcagcc tataaggagc aggaagtgcc tgttccgtct gtgcatattg
    10861 tggggcggcc caaagttgaa acagggcagc ttgctgtcct gcatgcggta actgcccacc
    10921 tgacggagcc catgactttt ttctggtata aagacggtgt gcttctgccc ggtgccgagt
    10981 tccgggatta cacgatacct tctgtaagtg ctgctgattc aggcagctat gtggtgcagg
    11041 tgagcgatga ttctcagggc gttttcaaca gtccgccctt tgtgctggaa gtgctggaag
    11101 aagggagcct gcctgtcgcc ggtgcgcctg cgcttctgct gcttgtgctt cttctcacgc
    11161 tggcgggcgc gtttcttttc ttgaaccggc tctcttctat aaaataggcg cactaaggtg
    11221 tcgctgatgc gggtggcact gtaccgggca tcccggcgat gcctgaggaa aggcgcttat
    11281 gaacagatcg ggatttttaa ggacggtctt tgtccttctg gcggtgtttt gtctgctcca
    11341 tggggggcag ctgcaggcgg aagaacgtat tctgctgata ggggacagct gggctcaggg
    11401 catctggatg gccggtttgc tggataaagc gctggcagaa gcgggctttc cggaaatgac
    11461 tgccatcggg gaaagctgcg ctctgggagg cacccgggcg gatcaatgga acaagccgga
    11521 gtaccgcgaa aaaattctgg atgcgctggc gctgtcgccc acggtggata tgatccatct
    11581 gatcattggc ggtaacgatg tgctgaagcg tatccgtgac acaaatgtat ttaccgcctg
    11641 gtcggagaaa aagagggaca aggaatggga tctgattgcc gcagatatac gggatctggt
    11701 ggaattctgc ctgtcgattg aacaggtcaa atgtgtaggg cttgccggtt acgattacct
    11761 gaatgcatcg acggcgaagg aagcgctcgg gatgctcggt cagaactttg attttggcgg
    11821 catgtcccag gagcaggtga atgcctgcat gattgcgctg gaaaagagaa aaaaagatct
    11881 tgccgcctct attaaaggct gtgtgtacat tcacaatttc gggctgctgc aacatcattt
    11941 taacgacccc gaagggacgc ctctgcccgg tgcgcctcct gagtatgtat cctttccggg
    12001 cggtgatccg gcccggccca tgccggatgc ggcatttact aaagtgagtt tcggcggtcg
    12061 tgaattttcc ggtgacggca tccatcccgg ggaagaggcg cacatggtca tgcttcggaa
    12121 tgggatgcag tgctgctatg tcccttattt aaggagcctg acggaaaaac aggcaacagc
    12181 agaacatgat gacagatccg gggggaactg atgtggctgc tttatggttg tcttctctgg
    12241 aatggacgct gtcgggctgg cgcccttttg cctggaagct gggaaaaaca gccccggagc
    12301 ccaatccgct gtgcccggac attttgcctg ttccggtacg atttcccatg tcagtccagc
    12361 aggcactgct ggaagcaggc atcattccgg actggaacaa ggggatgaac tcgcttctgt
    12421 gcgaatgggt ggagcatcgt cattgggaat acagcagtcg cctgcgtctt tcgccttccg
    12481 aatacggtga caaggtattt cttgatgcac ccgggctgga ttattcaggc tggattctca
    12541 ttgacgggga ggaagtcgtc cgtttttcat ctactatgag tgcacaccgg atcgacctcg
    12601 gagcggcgct gctggacgga acagagcacg aattgagcat aatttttgaa gagccgcccc
    12661 gggaacaggg tcagatgggg tatacctcca gatcccgctt tttcaagtcc cgctacaatt
    12721 acggatggga ttggtgtccc cgaattgttc ctataggacc ctggcttgat gcctgtatac
    12781 cggacagatt ggagagtcgg aagccggatt tttatgtcag cgcccgcctg gaggaggatt
    12841 tccgtcacgg tacggtggaa gtccgcctga tgttgcctga tgctgcagaa gagcgcagct
    12901 ttcgtctgtc cctctctgac ggcactgccc tgcttggaga aaagtcgttt tgtctcaaaa
    12961 ggggaggcgc acaaaagata cagctgaccc ggcttccggt ggaagcgtgg tggcccaacg
    13021 gtttttccgg acaaaagcgc tatacactga cgattgaaga aatagacggg tcgggcactg
    13081 tgctgtgggc cgggcacagg cgtgcgggtt tcaaacatat tgactggctc ccgtgcgaag
    13141 gcgcacctga cggcgccgat ccgtggctat gctgtgtgaa cggcgcaccg atttttttgc
    13201 agggcgccaa ctgggttcct ccccgagcgg tttaccacga cgcacagcgg gagcactatg
    13261 caacgctcat ccgcctgtac aaggatatgg gtgttaacct gctgcgtgta tggggcggcg
    13321 gcatactgga aaaggaagtc ttttacgatc tttgtgatga ggctggtatt ctggtgtggc
    13381 aggagatgcc tttatcatcg tccggcatag agaattgtcc gcctgaagac agcgaagcga
    13441 tacagacgct gcagaaaatt gcttcgaatt atgtgcggct gcgggggcat catgtgtctc
    13501 tgctgctgtg gagcggcggc aacgaactca cctggggcag tcccgacaat cctttcgggg
    13561 aaacgccggt cgatatgcgt catccggcta ttcacgcgct gaaagtacag ctggagcagc
    13621 tggatcccgg gcggcgcttt gttgccactt ctccgacagg tccccagttt tatgcacatc
    13681 ctgagaacta cggcaagggc cttcatcacg atatacacgg tccctggggt ctgagccgat
    13741 tcgagagggc agatttcggg gacagcctgg acgtctggaa acaatattgg gcggaggacg
    13801 attctttatt ccgatctgag gcgggcatgc ccggcgccgc cagtctggaa tgcctggagc
    13861 agtttgcgcc actcggtgaa tcctggccct tttctgaagg ctactggcgg catactgcat
    13921 cctggtggac tcaagaaagg ctgtaccggg cggtggtgca ggatggcatc gacacggcag
    13981 cccgctatgt ggctgtgacg caggatgccc aggcacaggc ctatgcaatt gcggcaaagg
    14041 catgcaaaag ccgattccct caatgtggcg gctttgtggt gtggatgggt cacgactgtt
    14101 ttccctgccc tgcgaacaat tcgctgattg attatctctg cacacctaaa gcggcataca
    14161 aatcactgca ggcgattttt ctggcgccgc cgggtgccta gtcgcttact tttgtctccc
    14221 tttcgattat ttagtatact ttctcttcgc acacgccaat aaaactgtaa aaatacgact
    14281 tttcgttttc agaacaaata ctgccctttt gaaactattg tgcgattgta ggggtcttta
    14341 agaatgctgg agagggttcg tgtagggtac atggatctct gttgtcaacc aaaacactac
    14401 aggagtttga ttatgaaagt actgctgaag ggaatgtttg tcggtctttt tgtgcttgcg
    14461 cttacagcgg gctgcgccag tgtgggctcg aaagtgtctg atcaggattt aattaaggat
    14521 cttgtgaatc agttgaaagt cgctctggag aataaagata ttgacggtat tatgaatact
    14581 ttttccgatg atttttatca tccggaagtg ggcggcaagg aagagggcaa agagatgctg
    14641 cagatggcac ttgatgcggg ctatgcggac aacggcgaag ttttcgtaga cgatatggaa
    14701 atcgagctga aggacgacgg caccgcaacg gtatatccga tcgatctttc cgccgatccg
    14761 ggatccatca gcattgagct gctcgtgaaa aaggaagccg ccggatggcg tattcttgaa
    14821 gtgcgccctg acggcatgta atcgcttatc ttctgctgtt tcttgtgccc cggtcttgaa
    14881 aggccggggc ttttttatgg gccttggtgc agaaatacct gagtgccgcc cggcggcccg
    14941 ttatttatct gttccgctca ggaagcgtcg gacttcttcc agaacatcgt ctctgccctg
    15001 ccattttttg cagaccaggc gcatatcgag ggtgtaggag gtgccgggtt cacagtcggg
    15061 aataatcatt tggaaatccc aggcggggtt ggtgtcatcc cctttttctg tagcgccagc
    15121 cccggacgga gaatgagcga atcgaatcaa cggcccgggt tgaaagatat aaatgagcac
    15181 ctgctccctg atgcggccgt aaaagaaggg gacggcatag cgcaggggtg agatcgccgt
    15241 gaacagggtt ttttccgtac cctgaaaagc aatattcacg cggtcgtttt cgctgcatac
    15301 agtgctgtct ctgttatgct cctgtgtaca gaattgaatc cagcgcgggc tttccagtga
    15361 ggagccatca cccagaaaat aaatgctttt gtcttccggt gcattcatgt aggaagccca
    15421 gaaaataccg agcaaaccgc ctgccagatc ggagcggtgg ggtgtgcagc tgaagtgcat
    15481 gtcgatataa tgaggtgctg tggccgtgaa gcgggtacgg ctttccacgc cgaatacggg
    15541 cgtaacaggc tggtatagct ctgcggtgtg ctcatcgatg cgtgtgaact gcatggcggc
    15601 acgtcggggc tcaaaaaaga cggagccggg ctccgttacc ggaagcgcat caaagtaatg
    15661 ttccagattc agtcctgaaa aaagcggaac gaacacattt tgtccgtctc ctgaaggaag
    15721 gagtgagaag atgccgttgt acccgcgcat gtgcggatca gcggctgtgt tgtcgccgat
    15781 ggtgcactga aagcccggca gtgtgaaagt gccgtaatgc gcctccattt catcatcagc
    15841 cccggcattt acagggtgaa ctgccgccaa tgtgccccag aggatcagcc ctgccaggag
    15901 catgtataag cggagagtca tttcttttcc tttctgaaaa tccgatagat tagagataac
    15961 gctgcagcag cgagagcatg gcgcgatcca gctggtgccc gttccacgct tcatacgcca
    16021 catcgctgcg cccgctgtcc agaataccga aggcgcccct gaaattccaa agtgcccagc
    16081 cccagcccgc ttctttccac agctccaggc agtcctgcat ccaggcgagg gtgacgctgt
    16141 gcggtgtctg attgaaggcg ccgaattcgc ccaccatgac gggcatgcct ttttcttccg
    16201 ctgccttcca gggggcaatc tgtttttctt tcagccagct tttatccatg agcgtttctt
    16261 ttttcctgag cggataggag ggcaaaggaa actgatcggc gccctgcacc caggatgcgt
    16321 gataatgagt cagctcgaag ggctcgtaac cccgtgtgga agcggcaatc ccgagcggca
    16381 tcagttcatc gggcggcact ttgccgtaga gtctgccgtc gcagatgatc agtcggctcg
    16441 catcctcgtt tcggattgcc tggacaagag cttcaattac ggcataatgt ttttttgcgg
    16501 ttaccagagc aggttcattc agcaggttaa aactgaggcg gtcattgggg atcccggcat
    16561 aacgccgggc aaaatgccgc cagtgaaggg cacaggtttc cagggcttcc cggtcgtccc
    16621 aaagattctt ttcttcgccg ggctgagcca ctgtataccc cggtgcacga tggaaattaa
    16681 tgcagacatg gatattgtag cgctcgccga attgcaccgc ttcgtcaatt tcccggagca
    16741 cctcctcttt cagttccgtc cagtcgtccg gatcggtcca gtgccggtag tccatgggca
    16801 ggcgcacaaa attgaagccc gtttcctgaa tccaggcaaa gtctttttcc tcaaaggcgc
    16861 cgcaatgggg catcataaat ttttcgagca gattgaagcc gtgccagagc ggcagtggtg
    16921 cctgttttcc ggaatgcagg agggactccg ccggtgcata aagagcagta cctgcaaggg
    16981 ccgcggtact cagccccaga aaatgccttc gtgtcatgga tagtgcgcca aggttcatgt
    17041 tgtattcctt caatgaagtg gggcgtcgtt gaaaatctgt gtgtgcaggg taatgcgctc
    17101 cgtgcctgtc atgatagcac gatgttttcg ttttgtgaaa ccgggattcg gctttgtttt
    17161 cagttgcata tccttcagga ttacagtcat aatgatagac gataaagatt ttcgcccggt
    17221 cagcgggcac tcaacctgcc gcactgatcg ctgagatccg gaaaacattc aaccccgagg
    17281 aggattttgt atgcgtaaga aaagtattgt tgcgcccgac tggtgggatt atacgacgct
    17341 ggatgatgag ctgctgaatg atgtggcgaa tctgacggaa aaagatctgt ttcagttgtc
    17401 ccgggacgga ttcaaagttg tgtttcatga ttctctcgaa tccttctatc tggctgaagc
    17461 gcttcagtat atcgaagcct ggaaaagttc cacgcccgac aatccggcag gggtctgcgg
    17521 tcccatcggc cccactgagc agcttcctct ggttgccatg ctggtgaacg aattgaacat
    17581 taatctgtcg aagctgggcg cgcagttctg gggcatggat gaatgggtga aggatggcaa
    17641 aacgctcccg gcggagcacc cgctttcctt tcagaaggcg aatctggatt tgtgttttaa
    17701 cctgatgcga cctgagctgc gtccccgccc ggaagccatc cattacccta taggtgattt
    17761 tgccgctttc agcaagtcct ttgatgatat gcgctgtctg gtcatgcagg gcggtcaggg
    17821 agatgtgaag cactgggcgt tcaacgaccc catgaagcgg gaaggcgtct gggcggatgc
    17881 gccgccttct cctgaagagt acctgaagct gggtacccgg gtcgtggatt tgcatcccat
    17941 gacgatcatg cagaatgcgc ggacgtcggg cggcggtcgc ctgtcggaaa ttccgactca
    18001 ggccacgacg gtcggtccgg ctgaaacctg gaaagcggag aaggtatcga tctggcaggc
    18061 gggcaatcat gacaatccct tcgggatgcg cctgaccgct ttcatgatca gcaaagggct
    18121 ggcggactcg gctgtgccga tgagccttct ggcactgcac cccaatgtgg aatttcatta
    18181 ttaccgcaag gcgataggca gctgcggcat tgagatgcac tgacggatcc gacgatgcgt
    18241 cttgtagatg tgcactgtca tcttgacagt ccggcttttg agcgccgtct tcacgatctg
    18301 ctggagacgg cgcgctcagc cggagtctgt gcgtggatca gtgctgcaat ccatccgtcc
    18361 ggctgggagg ctgtccggat gctgtcccgg tgccatgccg gggtttatca tgcactgggc
    18421 gtgcatccct ggtactgctc cgacgacagc cttcctctga tcccgtctct ggcatctcag
    18481 gcgtcagaag cggtggcaat tggtgaaata gggctggaca gcaaacgacc ggtatttccc
    18541 ctggagcgcc agctgtctgt ctttagccgc caactgggca ttgcccggga actgaagctt
    18601 ccggtgatcc tgcattgccg gggcgctttt aatgagatgc tgcagctttt ccgaaaggaa
    18661 ggggcaccgg cgggcggaat cctgcataat ttttcgggcg gcccggagct cgcgaaggca
    18721 tttatggact acggtctcgc tttcagtctg gggggcgtgc ttacctggcg caacagtgcc
    18781 cggcgcacgc ttctgctgaa ggagatttat ccgcacagcc tgcttctgga aaccgatgcg
    18841 ccggacattc cgcctgttga agcacggggc accgtgaatt caccggcaaa tattctatac
    18901 aatctgaaag cggcttccga actgctggat gtgccggcgg aagagattgc cgctttcagc
    18961 acagccaatg ccgggcgtat tttcggcata gcgctttaaa ggattctcct atgactattc
    19021 tgactctgct tgtgactctg gccgcagctg ccgggcagct ggctgatccg tttccgggac
    19081 cgccccggca cggacttgtc tatgtgatcg cccatcgtgg tgttcatgac ggcattccgg
    19141 aaaatacggt ggctgcctac aaaaaggcga ttgagctggg agtcgattat gtggagattg
    19201 acgtccggga aacgaaggac gggcagctgg tcagcgtgca taatgccacg gtggacgcct
    19261 atacagaaga tgcaaaagga ccggtaaaag actttacgct ggcagaactt caggcaatgg
    19321 atataggcag ccgggtcggt gatgaatgga aagacgagcg catccctaca gtggacgcta
    19381 ttctggaggc gtgcagcggt aatgtgtcaa tttatctgga tttcaagcag gcgccgccgg
    19441 aggaggttgt cgacaagctg cgggcgaagg ggcttgcgga aagtactgtg tggtatgcag
    19501 gcggaggtcc gctcagggcg ctgaacaagg tctgtccgga atgccatccc atgccggaag
    19561 cgggctccgg agaaaatctc gacaggctgc tgcagcttca cagaccgcgc gtggttgcat
    19621 ccacctgggg tgcagcgacc agagagatgg ttgaccgctg ccatgcggaa ggcgtacttg
    19681 tatttgttga tgacggagga ccggacacct ggcaaaccct ttttgagtgg ggcgtggacg
    19741 gcattcagac agatcatgcg gcagaactga ttgaagcggt caaagcgtat acagcttcgg
    19801 cgaaaaaaga agaagattcc ggcggggttg ccggagcgcc atgaagccgc ggctgtctgt
    19861 cgttgttccg tcgtatctgc cggatccggt gctgctgcgc aagttgtatg attcactgca
    19921 ggcacaggat tttaccgact ttgaacttct gatcgttgac gacggttcgc ccgacctttc
    19981 cgcctatgat tttccggagg atgcgagggt ccggcttatc cgtcaggaaa aaaatgccgg
    20041 tcctgctgcg gcacggaacc ggggcgcggc tgaagcccgg tctgatcatc tgttttttac
    20101 ggatacagac tgtgagcttg ccgggggaac ccttgccgcc gcttccgccg ctctgcagaa
    20161 ggacgacatt ttttcaggaa atacccgcac cgcagtgaag acaaggatcg gcagagccat
    20221 tgctctgctg ggctttcccg gcggtggcat cctcgggttt gaccgggtgt ggcgggtgga
    20281 caaagacggg tacaccagga gtttcagcag ctgcaacctt gctgtaaccc gggagcggtt
    20341 ttttgcgctg ggcggatttg atgagagttt tccgcttccg ggcggggaag atacggtgct
    20401 ggcggctcag gcatgtgcgg caggttttcg tatacgttat gtgcctgaga tgcttgtgta
    20461 tcatgtggaa aaaagcacac tgggcagttt tttccggtgg cagctgatcc ggggccgcgg
    20521 caactggcat atcaaaaagc gcctgccgtc cgtgggcggt tatctgcggc ttcgcgtctg
    20581 gacattcagc aacagtctga aagcggcagg gatcctgtat gccgtgccgg tgctgctgct
    20641 catcgtgatt tctgtagtgt tgcaggtgac gggcatgatc cttgaaaaaa gacggatgaa
    20701 aaaagacggt tccccgtaag atataataca gtcaggatct gttgagtgtt ttcattcaaa
    20761 aggcggaaaa gaaagtatta tatgcaaaaa aatgttccga ttgttctggt gctggttctt
    20821 tttctccttg gcggtgcagc cagcggagcg cctgcacgcg aaacgcctct gatacggtgg
    20881 ccagctgtgg cgggtgtgaa ctattcgtca gatccggaaa cacttctctc gactcttgtg
    20941 aatttccgga tgcaggcgga tgttcctgaa atcaataacc gtctggtggc gattatcgca
    21001 tcgcccggcc cctacgggct tgcgggtaaa accaccgccc atgcgttcaa gtctctgcag
    21061 cccggacagt acgggcgcgt ggtgatactg gcaccggggc atggtccggg atttgaaaac
    21121 tgttccattc cggcagtaga tatattcttg acgcccctcg gacctgtgcc tctcgacggt
    21181 gccgccatac gaacgctcat gtactcgccc ctctttcaaa tgcatcagat tcgctatgac
    21241 ggtgccaagc ctcatgagcg tctgcacgag tacgagtttg caattgaatc catgctgccc
    21301 tacctgcagg aacggcttct ggagtttaaa ctggtacccg tgctggtcgg cgatttgcgt
    21361 gacccgcagg ggaaaaccac ggaaaacacc atggcggctt ttgccaacgc cattaaaccc
    21421 attgtggatg aacggacgct gattgtcgtg gcaagcagtt ttacccacta tggcgaagag
    21481 tacaaaaata cgcctttcac ggaaaacatc gaagacaaca ttgcccatct tgaccggcag
    21541 gcctttgaag agattctgtc gctgaatctg acaggtttcc gcgcctatct gaaaagaagc
    21601 aaaaacgtga ttgacggtca ggactgcatc cagattctga tgcagatgct tccgcctcat
    21661 actcaggcac gtctgctcgc ctacacgact tcaggcgctg tcagcggaaa ctggaagtcc
    21721 tccgtcagtt acggcagctt tgtgtttcat gatctggatg ccccggcact ttctgcccgc
    21781 ccggaccagg tgcgccctct gattcttcgc aagcctgaaa agacggcggc atctgcaggc
    21841 gaagctccgc cggcgggagg gcagggaaaa acggatacag cggtaccgta aatcaggagc
    21901 cggacatgga agaaaaagag attcagctca ccccggatga aaaaagcctt ctgctgcgta
    21961 ttgcccggga atctgtacag gcgtatctgg aggagggcgt tcctccggca atggacactt
    22021 atccgctgac ccctctgtta aaacaaaaga tgggcgtttt tgttacgctg cgctgtgcgg
    22081 gggcattgcg cggctgcatc gggcatattt ttgcgcagga agggctcgcg gaaacagtgg
    22141 cggtcaatgc cgtcaatgcc gggttttacg attcccgctt tgcaccgctt cgtcccgagg
    22201 agctggaggc gctgcgtttt gaggtgtctg tgctgcttcc cgccgaggag gagagtcctt
    22261 ttattcctgt gaatgatccc ggcgaaatct gcatcggacg ggacggtctc tatctggagc
    22321 acccggaagg ccgtgggctt cttttgccac aggtgccggt ggagcagggc tggaatggca
    22381 ccgcatttct ggaagggatt tgccggaaag cgggcgttca tgccggaggc tggcaggatc
    22441 ctggtgcccg gctctggcgg tttcgcgcgt gtgtgttcgg tgaatcagag gactgaccgg
    22501 gcggtgcatc agactgactg cagccgtgcg ctgatccagt cgataacagt ctgcatgtag
    22561 cgttctttat caagatcatt gtagatctcg tgatacgcat cttcaaagag tttcagttcc
    22621 ttgtcggcgg caccggcttt ttcgaagagc gctttgcttc ctgaaggacc gacgatacgg
    22681 tcgcagcagc cgtgaatgac cagcatcggc acggtgatcc ggggcatttc tttttcaatg
    22741 atgcggatgg tcctatgaaa ctcccgtgcc gtgtgcgcag ccactttacc gtgatagctc
    22801 agcggatccg catccgtcct ccgctgtact tcaggatccc ggctgagccc tgaattgtca
    22861 acctggctga cgggcatcca gggggcgagt gcggcaacat acggagtgag cgcaatcagg
    22921 gcggcgggca cctcatcact gaaagcgagg aaggggctgc tgaaaatcag accacggaca
    22981 tcaggctgtc gggttgcggc ccagcgagcc agtaccatgc cgcccatgct gtgcccgaag
    23041 acaaaaagcg gagcggaggg atcgctgtct tccgtaacgc gtttcagaaa gatatccaga
    23101 tcatccggca gctcgtcgaa acgggcaaca tagccgcgtc tgcccggtga tctgccgtgt
    23161 ccgcgctggt cgtaggcata ggttgccatg cccgtgttct gaaactgttc catgacccag
    23221 ctgtagcggg cgcaatggtc gccgtagccg tgcagtacga tcacgcgcgc ccggggcggg
    23281 gatggtgtaa ggagatgccg ttcaaacaat ttgatacccg gggcaacttc aaaccagcct
    23341 tcctgaaaca tggggagtcc tttctacagc gccgtatcag cagcagctga tgcgcacctg
    23401 ttgttctgcc agcacagcac cagcggcggc ggcagtacat aatcacgaaa cacgccggtg
    23461 atttccattc tgctgatacc gggctgaatg gtaaaccggt attttcccat gcgggaaagc
    23521 agctctcccg caccggtata ccagccgtca aagtgatatt ctgtgttgac tctgatagcc
    23581 tgcagtgtga ttgtcatgtt ttccgcaagc cggtacacac cgggcggggg gctgatctgc
    23641 ccggcaccat tgccatcaag aaaaacggtg agtacggcgc ctctggatcc ggtctgcagc
    23701 ccgactgttt tgtctctgtc cagacaaagc attttgtctg tataaacagc atcctggtcc
    23761 acatcgcccg tccatccgcc gaagtatgca tcatcggaca ggatggcagt gagaggaatt
    23821 tgtgcgtttt tggcgaagtg atacaatccc ggatcaaagt ctctgatgac tgacaaagac
    23881 gagccttcag tacgcagcac tgtcaggcgg acatgctctg tttcttctgc cggtgcaaag
    23941 cgtgcggtga ttactttgtc tccgtccatg atcagattcc ggaccggatc ggggctgagc
    24001 agatctcctt cccatccgat gaaaaccaga ttggaattcg caggcggtat ggccataagg
    24061 ggcactgctt ctccgtttgt gcgaccgctc agcccgggag ggggaaagag cttcggggca
    24121 tgcatgccgt cagggagatc cagccggata tcgagctgcc agtcggcagg ggcaaaccgc
    24181 gcggtaagcg ctttatcctg atccatcaga acaagaatct tcggctgggc aggcagtgcg
    24241 tttgcaggaa gatctccttc ccagcacaga aacacagcgt ccggatccgg cgtcgcatgc
    24301 agctgcacat aggtgccttc ttcaaactgt tctataccct gccagtcccg gtcgatgtgc
    24361 cctttgcctt cggcagtgag ctgaagcgaa tacagaggca gtccgaagac ggcggtgatg
    24421 ccgtcgtctt cttcaagcac tacgcgcagt aaggggttct cggacaggat tcctttttta
    24481 aaagagcgcc agtagtggaa gatcagccct tccggcggtt tggcgatgaa atcggcaggg
    24541 gtaccgacca gtacagtata gtcgccgggt gccgggatga tttcagcttc agggtgcgtc
    24601 gaggcgaggt gcagagagca cgcccctctg ccgaaaaccg cagatgcatg catgccctct
    24661 tcagcggtta cagtcaggga aggtgtaatg gcgacctgct cttcaccgct ttcgcgggta
    24721 attttccagc cgccgaaaaa acgctccgct gcaggcacag cctgaagggt gagctgcgtt
    24781 ccggcaggga tgcgatagtg tcccggtgcc ggggtaagcg tccccgggga gcctttttct
    24841 ttttcgacag aaagagagac cagattgtgc cgggtgaaac atgcccggac tttccggctg
    24901 gcggtcatgg gcagatgcag cagcctttgc gttctgtcgg caccttccgg aagatcgcct
    24961 tcccatcgga gaaaagcaat gtcgagatcg gatatccagc aggcttccag cgactgaata
    25021 tccttctcct gtccggtgaa tccgtaaagg cccggcatgg gctttagcgc acctgcagga
    25081 agcatggcgg gctctccgtc aatttctgct tccagatcaa gaaagcactg cgccggtaca
    25141 aagtgcgcgg taagggatgc gtccgccgtt accggcaggg taaggagagc gtcgtgactg
    25201 atgccgtcat gggccgtgcc cgaccagccc gcaaaggcac tttcatgatt aagcgttcgt
    25261 gcttccagcg tgagtgtgct tccgtaaagg gcgtgcagta ccggcggaca gggcgaggcg
    25321 ccggaaaaaa ccagacctgc tgtcggcggc ggagaatcta cacagacctg aattcgtttt
    25381 tcaaggggtt caaaaacagc ccggtatgtt ttattctcgt taacccggaa agaaaaatca
    25441 gcccagcgcg gattgtctga ggccagaacg atctgtcctt tttcgtcttc tatgcgggat
    25501 aaatgccacc cttcttcagc ggcggcgcga agagtcactt ccgtgcctgc tgccagagga
    25561 tacgttccga cggggacgcc ataccagccc tgcccttctg tttccagtgt gatgagcgcg
    25621 ccgctgcggt agaaccaggc aatggcggta aggtttcttt cagtggggag tgtacaggtc
    25681 ggaaagggtt cccggcgggc cgaggtgctt tcgccagcac cggtatcttc ggggagtatc
    25741 tcccatccgc cgaaaaagct tcccttatca ggcagagcgg taaacgcctc cggccagtcg
    25801 ttgcggaata cccaactgcc cggggcgggg tctgtggtgc ccgatcccgg cccggcgcat
    25861 tgtatggtca ctttctgaaa ggagacaccc ggcggcggcg tatcggaaaa gatcgctgta
    25921 atgctgcgtt ctctgccttt catttccacg gtgatttccg ggctggtgct tgagatggtg
    25981 cctgcccatt cacggaaccc cagtccggta ccgggcaaag gccgtgcgag aagattcgca
    26041 tgataatcac gtcccacatg gagcgttttt acgggcggca gaacgctgcc ttttccctgg
    26101 atatgaatat ggaggcgttt gaagtcgttg ggcagcagga ggacgaaagt gaggaagatg
    26161 acagcaaaaa tagccagcct tttgaaaaag gtgaatattt tcttcaaaag cgggtgtgcc
    26221 ataagaacgt tgtcatccgg ttcagaaaag ataattacgg aactgctgcg tgcagcgtat
    26281 cattttccac tattggaagg aaggaaatcc atcagagggc ggatacggct ttccgggaaa
    26341 agccttattt ttgagacgct gccagacccg atgcttccag cgccagaaaa cgctccttcc
    26401 acgggaggcc gtaataatag ccgccgagag taccggaagc gctgagcacc cggtggcagg
    26461 ggacgaccag gcaaatggga ttcacgccca gagccgatcc ggtggcgcgt gccgccccgg
    26521 gatgcccgat gcgctgtgca agtgtgccgt aggtggaggt gcccccgtaa gggatgcggt
    26581 atgcttcccg ccatactttc tgctggaagg gggtgccgcc ggacatatcg agaggaatcg
    26641 catcgaactg aacgggcgtg gcgctgaaat agtcggcgag gagccggtgc agtgttttgt
    26701 cccaggaagc tttgcgtgct ttatcgactg ctgcaggcat ctgtttttca tcgggaacca
    26761 gagacagccg cttcaatccg gcttctgaga aaaaaccgca gaaagcgcct ccgacaaagc
    26821 ggaagcagaa gtttgtgcat gtctctgtca ttccgatgct cctgagataa aaaaaagaag
    26881 cgggcctgta agccgggttc tgtccgaaac gcttgcgcat ttctgaacgg tcattcatct
    26941 gggacgcatg tcaccatgcg cctctagcgt cctacccggg accggtgcgg gcaacaccat
    27001 gggtccccta tttggacttg ctccggacgg ggtttggcaa gccgccgtgt caccacgacg
    27061 ctggtgcgct cttaccgcac cttttcacca ttgcctccga agaggctgtg tattttctgt
    27121 tccactttcc gtcggatcac tccgccttcg cgttacgaag cgtcctgccc tgtggagccc
    27181 ggactttcct cgacacggca aaccgtgccg cgaccgttcg gcccgcttat ccattaagtg
    27241 taccgcatgt tccgttaaaa agtctatgtg atttcagaaa aggtgtcgta actctttaga
    27301 aatgtcccgt ttcttgtttg ttatcccgct gttgtcccgt ccatgcattg aactgatgcc
    27361 tgttccagaa caagttgggg gcatgcgcct gtttgtccgg cggataaccg tatttgcgaa
    27421 gaatgcggta ctcatgcttt cttccttccg gtctggcggg tacggtagag gcgttccgag
    27481 aatccgccct cctgctcaaa ggcggcggct tgcgatgcca tctgacgatc gagcaacacc
    27541 gatgccacct ttatcagaac caatgcggca tttgccgaat acccggaata gatggactcc
    27601 agagaccgga tggacggctc ttcctgcctg tcgtttgtag aaatctcctt aacccagacc
    27661 gccatctcat ctgccgtcat gcatcgccgg gcaaccagcg cttgtcgtcg cggatcgtcc
    27721 ggctgc
//
LOCUS       DAOPWB010000025        27539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1716_length_27539_cov_5.684325, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000025 DAOPWB010000000
VERSION     DAOPWB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 27539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27539
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1716_length_27539_cov_5.684325"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1143
                     /locus_tag="PKN92_03940"
     CDS             196..1143
                     /locus_tag="PKN92_03940"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ47976.1"
                     /translation="MGIMSKLGLHKGSAKTASPGQLGGAAGLHSSEFELSHVGPYQIQ
                     APIGSGGMGTVYKALDPARDVTVAIKVLDRRYDLDKKKSKQDYLGREVLIAASLNHPT
                     VIKMEKEIINQEDVEGNLRRCLIMEFIDGYNLKHFIDNRILTIKQMTNLTIRLAEGLD
                     FLHQNGVVHRDIKPANFLISRDMSQVKICDFGLSKSSATWRTRWLKEKGGTRLYMSPE
                     QLRKQTLDARSDIFSFGITMFELFTGRHPCAGNNPREIQKQLLSSKYRFPAPSSINRE
                     VPERMDRVILKALRRNLDTRYQSCTEMLLDITRVSGSRI"
     gene            1149..2444
                     /locus_tag="PKN92_03945"
     CDS             1149..2444
                     /locus_tag="PKN92_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNZ47977.1"
                     /translation="MKKRIFSKKSRWEPGLFIIALVVAAVMAVIIAAPYLLSKTKEAF
                     PVVLPVSSDGLEVLQQAQKNVASGNYDEAMAQIKPLLKGRNPLTTPRAIILEADILHY
                     KNQPETALANLTAACDAFKSSPEYPYLQARKARQLEEMSRTAEARDIYESIRDHAPAE
                     IRSLGYLGLARLDQRAGNLVGARDLARRAAEDSPWGTVVWNEAIDMLGPLNVALIFSP
                     QETPESKIYTVEKGDNLNSIGIKLNTTQGLLMEANSITSPEKLSLGQRLKYTPKDFSI
                     IIERSTCSLYLMDNKGIFKRYKTGLGKPGHETTLGKYTIGTKQKDPVWHKPGAGPIAA
                     NAPDNELGTRWMPLEPAAEGLPRDLGIHGTIAPETIGSFASKGCARMLNEDVEELYDL
                     VVRATPVEIVETLDPNTQQAPTVASSTPAAAEAVQTAAE"
     gene            2555..3292
                     /locus_tag="PKN92_03950"
     CDS             2555..3292
                     /locus_tag="PKN92_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47978.1"
                     /translation="MFCKFCITDNAVECMTPDDFAETLTVLEKAGFTNIVIGGGEPFT
                     WPYGLHNAAKIAKSKGFLVQVGTNGIKMPDGAAYPDCVDRYVLPLDGTTRTEHNRVRH
                     FPHQIGGHFQIIMNRLAQLKEAERSVTVSTVVTRHTVANLHTLGAFLADYVQTGGILH
                     AWHLYRFLPAGRGGAKSAEELSISSAEFEDATTAVKDQHYPFTIFKRRDMRHSAHVDF
                     FWRQKGTVLRGSDYWKQTAALSPIGAF"
     gene            3419..4813
                     /gene="hydG"
                     /locus_tag="PKN92_03955"
     CDS             3419..4813
                     /gene="hydG"
                     /locus_tag="PKN92_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009380767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNZ47979.1"
                     /translation="MSDELYGTVTIDEDAIQNALDHSSEDHAHIREVLAKALELKGLN
                     ADDVSALMRIQSQELEEELFETARKAKMEIYGRRLVLFAPLYVSNLCANDCTYCAFRS
                     KNKAVKRRALSQEEVAEETRIIINQGHKRILLVAGESYPREGIEYAFKCIDTIYNTKS
                     EWGEVRRINVNLAPLTLDEFQGLKDRRIGTYQLFQETYHRDTYSKVHLSGKKRDFDWR
                     ITAMDRAMLAGIDDVGIGVLFGLYDWRFELLALLQHNTHLEERFGVGCHTISVPRIEP
                     AVGSDLSEHPPVPVSDKDFRKLIAILRLTVPYTGIILSTRENPEMRREALALGVSQIS
                     AGSRTNPGGYHEESYDMSQFSLGDHRSLDEVVEDIAHLGYIPSFCTGCYRLGRTGKDF
                     MDLAKPGLIKHYCEINALSTFAEFLEHFASPATQEVGNELIDRIIEEQPPTRKERAST
                     VVRKVRAGDRDVYV"
     gene            4882..5145
                     /locus_tag="PKN92_03960"
     CDS             4882..5145
                     /locus_tag="PKN92_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47980.1"
                     /translation="MKDRHIIYGVHVQNRGENAVRVQQLLTEYGCFIKTRLGLHEVDE
                     QFCATGGILLLEMFGDIARCEALRDKLNSIEGIEVQEMIFDHS"
     gene            complement(5181..6215)
                     /locus_tag="PKN92_03965"
     CDS             complement(5181..6215)
                     /locus_tag="PKN92_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ47981.1"
                     /translation="MSDREKNYIRRAEEIMLSKSQKNVVFKVLQAMEDGCLCIEGVYD
                     EEDEEIYFTGSVFFWIDATSRYTADDETFLKDLYWCGTYSYTTVKDMPKTVHRYSSDR
                     ALAVSLVRINFDLFDEEDEEDADDDSEEDDLEITGSGTGFFVTENGYLLTCRHVVEDA
                     GAVAVLLPDEQVHAAKIIHTDKEKDLALLKVDGTFTPVTIGIPDEVQPGKTVFALGYP
                     NPEIQGFAPKVTMGIISALTGMHDDENNYQIDAAVQPGNSGGPLFFTSGEVVGVISAR
                     VNDGYYAEATGSIAQKINYAVKMEAVLSFLREEPAAFRRLKRGTSSASSQEEAVRKIT
                     RSLALVVTLK"
     gene            complement(6447..7703)
                     /locus_tag="PKN92_03970"
     CDS             complement(6447..7703)
                     /locus_tag="PKN92_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNZ47982.1"
                     /translation="MNKVCVLGLGYIGLPTAAVLSAHDLEVIGVDLNPDVVKTINQGA
                     IHIEEPGLSGWVQEAVSSGKLRATGSPVPADVFIIAVPTPITAECKADMSCVRAAAQS
                     IISCLRPGNLVVLESTSPPGTCRDLLAPLFESAGFRIGTDLFLAYCPERVLPGKILTE
                     LVENDRVIGGMDESSTKKAIALYDRFVRGKLIATTATTAEVVKLMENTYRDVNIALAN
                     ETALLCEELGVDYWEMARCANHHPRVHLHSPGPGVGGHCIAVDPWFLVEANPEGTSMI
                     QEARRRNDDMPRKVAEKALAMTASIEKPVIVVLGLAYKADVDDLRESPALKVTEFLQQ
                     ARVDLRLCDPFVKTDRYPVQSLESCLQGADMILLLTDHSEFKTVSPVQASEWMRNRKV
                     FDTRRALPRDSWVQAGFEFTCLGVGL"
     gene            7934..8554
                     /locus_tag="PKN92_03975"
     CDS             7934..8554
                     /locus_tag="PKN92_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNZ47983.1"
                     /translation="MNKIAELFAKAGSIQEYAKLYNARLSEVLASLDWDSFAKAVTAF
                     EEARQSGKAIYVMGNGGSAASCAHLVNDLIAGAYLDGKPAFRAVSLADNLATVTALAN
                     DSGYESIFVHQLKVLLRPGDLVLAMSVSGNSPNILRALEYAKSEKAVTVGFCGLTGGA
                     MKNLCDVVIHADTTLDEYGPVEDVFSILGHILAGYLSMKQGKMLHH"
     gene            8589..9428
                     /locus_tag="PKN92_03980"
     CDS             8589..9428
                     /locus_tag="PKN92_03980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ47984.1"
                     /translation="MHASIIIAVYNRREDVHACLSALPVAELTQKNTEVILVDDGSTD
                     GVREMAAQEFPHVQTLRLEDNKGPSFARNLGAKVARGSLLIFLDSDAVPEDRWLDTFL
                     LCDDGNTVLIGRILDYHTGKDQDRPRRSTFLGKSLPCSPRHANAGSSCNLALPASLFS
                     AIGGFDPDIPYYFEDSLLCIHARRHGARFRYLSDARVRHKGSGTRQGDTIRMQEHNST
                     YAMLMLYRHSFGRVLLFTAGNGLWLLIRLFVRLLTGRLHDAVLLAKGWGSAYSRFVSR
                     TGD"
     gene            complement(9409..10644)
                     /locus_tag="PKN92_03985"
     CDS             complement(9409..10644)
                     /locus_tag="PKN92_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ47985.1"
                     /translation="MAMPSNRDDNSKGYTVLFAPFLYLLRDYGLPVGLRELLELNQGL
                     QKGIVSTLDDLFIFSRLVFVKRVEYADAFERAFALYFYDIDIPAVQEGDDALFSTEQF
                     RRWLEEEIRTGRMPRHAFWHYEPEELMKRFWDTLKQQMEEHHGGSRWIGTGGNSPFGN
                     SGNASRGIRVMGASKNRSALRVIGDRRYVEYSDRNQLREENLRQALASMKHMQHAGPF
                     TRLNLDKTVTMTARNGGEIDLVFERDLRDKISVLLLIDNGGYSMLPYIEITRILFSKM
                     HERFEDLQCLFFHNTIYEKVWTDFRRTSPLDTEQLLLRRPDTRLVIVGDASMAPEELE
                     ARGGALSNFSDFSSPPSSTWLHKLSRRFPWSCWLNPIPREYWMRTHGAYTIENIGRIF
                     PMVDMSLGGIKEMVSRLSG"
     gene            complement(10648..11475)
                     /locus_tag="PKN92_03990"
     CDS             complement(10648..11475)
                     /locus_tag="PKN92_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNZ47986.1"
                     /translation="MNSYHFDGTDRYHLDAELKAVVNIALAMEKPLLLTGEAGTGKTQ
                     LALETARALGLPVEILRCKSTIKGEEACYVQDTVLRLNDARFGSGESGRRVDQFYDYV
                     IWGPIGRAFRADRKVVLLLDEIDKTESDVQDNLLDILEECQFTIREVNETVKAQIRPA
                     IFITSNAKRELSDPFLRRCFCHHIAFPAPEEMRAILALHYPNTHPELIETALKIFYEL
                     RDRGFEKPPATSELIDWIGALERDGKMPDPEFPPLTGLLLKRSADILKYRQRRFGRH"
     gene            11559..13046
                     /locus_tag="PKN92_03995"
     CDS             11559..13046
                     /locus_tag="PKN92_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ47987.1"
                     /translation="MANWLSYTSTEGASPRLAAVIILYEQQRQRVLFVKRNPSLPFMG
                     GHHAFPGGSLSDADTGSRVINAPDLRSARLLTTAVRELFEETGILLPDLTEAENGSLQ
                     SLREKTVSEPAVFEAFLEKKNIFIDYLNFSPAGRWVTPSFSPIRFDTSYFFCSTSKPC
                     FAAPMGAHAEIVGVEWITPAEALKRRDGKSMHVSTPVVFVLQRLHTFPLPEALKRLRH
                     TPGFSNTLLDYIEPFPGIHLVPLQSCTLPPATHTNCVLIGEESIYIVDPGASETSEIS
                     RLFTHIDEVCENVGGTPAGILLTHDHPDHCAAAEALSKRYNVTVYGHPASLGSFQNAE
                     ALEKGDFIEIPGSRPWSVRCLHTPGHHPGHLCYFEETTGALLCGDMIANPGTIMINPD
                     KHGDMGLYMAGLERLGKIPAALTVPGHGAPLMEGEGPALFQKTLEHRIMRETKIKTAW
                     EQGARCEDTLLAQAYDDTPESMHPFALLQLRAHLSHLQETDLISF"
     gene            13149..13814
                     /locus_tag="PKN92_04000"
     CDS             13149..13814
                     /locus_tag="PKN92_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020582283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNZ47988.1"
                     /translation="MKTQNTKTHKEYTVLEEIMNSGTHAVGFGLSVAGLVWLILRAVS
                     LKDSMSLVAVIVFGSSLIALYAASTLYHAVPCFNVKKVLRVFDHCAIYLLIAGTYTPF
                     LLVSLRGTTGWALFGVVWGIACVGCAFKAFFTGRFNKVSTAMYVAMGWIVLVAIKPAI
                     THIPVGGLLMMLAGGLLYTFGVFFYANERVPYNHAIWHCFVMGGSAIHFFAVIIYVLT
                     PIA"
     gene            complement(13827..14471)
                     /locus_tag="PKN92_04005"
     CDS             complement(13827..14471)
                     /locus_tag="PKN92_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNZ47989.1"
                     /translation="MKTIAQIIGAVVLIAALVLAVPVIHYFPMYNFRTVEEGAFYGSR
                     QMGGKAMERIIKKRGIGTVINLRGEQTDSDWYDQEVAACKHMGIQHVNFGWSKNSIPE
                     PESLAAFLDLMETGQKPFLAHCQGGTHRTGVAAAVYLLLKGADTATARKQFRIGFNDA
                     PIGELVTLYEGSPKPFKQWVLEDYPAKYEQWKKQREAQKEAAKVPALLAASALR"
     gene            14725..15981
                     /gene="hydF"
                     /locus_tag="PKN92_04010"
     CDS             14725..15981
                     /gene="hydF"
                     /locus_tag="PKN92_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNZ47990.1"
                     /translation="MRSTPKSMRLHIGLFGRRNAGKSSLLNAVTAQQVSIVSPVAGTT
                     TDPVEKAMELLPLGPVLFIDTAGLDDEGTLGELRKERTRAVFERVDLGVIVCQEGEWG
                     AYEEMLFSELDQRKVPVIAVFNKSDIKRPDTALLNMLSARKIASLCLSTITGEGLGDF
                     RQALLDSAPADFVEQPVILRDLVGAGELAVLVTPIDKEAPKGRLIMPQVQSIRDLLDG
                     DASCLVTTDKGLAATLAKLKEPPKLVVTDSQAFLKVAADTPSHIPMTGFSILFSRYRG
                     DLVTQVEGALAIDSLKPGDRVLIAESCSHHPIEDDIGRVKIPQWLMKYVQGELIFDSV
                     QGHDFKMDLSPYKLVIHCGACVTNRRAMLNRLLRCRKAGIPVTNYGLTIAYSLGIFER
                     ALEPFPDALAAYEKHKLYLKAKNTEA"
     gene            16035..17108
                     /gene="hydE"
                     /locus_tag="PKN92_04015"
     CDS             16035..17108
                     /gene="hydE"
                     /locus_tag="PKN92_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNZ47991.1"
                     /translation="MSDFLQHKSQEEIEGWLRETDPERLKLLWAAADETRRRHVGDQV
                     HLRGLIEISNYCIRACAYCGLRAGHGSVERYRLTRDEILSCAHKAVGFEYGTVVLQAG
                     EDYGIKRDWLAEIIKTIKKETNLAVTLSLGERPDEDLIEWRTAGADRYLLRFETSDRA
                     LYERIHPPAKGQEISDRPAILRRLKQLGYEAGSGVMVGIPGQNYSILARDILLFQDLD
                     LDMIGVGPFIAHPETPLGRGELESAPDGEQVPNTEGMTYKTVALTRLICPEANIPSTT
                     ALATINTALGRELGLSRGANIVMPNLTPPQYRALYEIYPAKACIDEAAEDCHACMKGR
                     IRRIGRIPGTGPGGRKKGGIAPQ"
     gene            complement(17117..20776)
                     /locus_tag="PKN92_04020"
     CDS             complement(17117..20776)
                     /locus_tag="PKN92_04020"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNZ47992.1"
                     /translation="MCKKSYFLLPAFFCLLTAVPSVNALPSYGKKAVPVPAGTAQPWG
                     KPLAGGAVKVLLVAPQHTLQDAAELKKRLDVDCRVVPLWDAEHAGYDPIAYGTISSAE
                     TDAEVFSRLEKELRQPADVLILANFNISLLPERILSEVLFKVSQGTGLLTAFDRSPVD
                     GPLAAFFDNLQEEADTDSISGGIGECAFPGGLELRQIIRSYVHGKGKIVCLDYPGDFP
                     RRNALVQSPADPYDLESAWEENAWSFVVRAICYAAGRRPEVRIARIEDGAPSGPAEDE
                     IPPDFYPEFIQAMKDSAVSQPSRPFVIRLNKPADKKYNVELQIRRPGSSTRITYEEAN
                     AFSKDSEFHIVEVPAGPGPCLFDVWLKDRSGITDWFTALLHVPGWPDFYQLELEKQWL
                     MPNDSLDISAKVRPFANQSRQACLYARATDGFERVVAQSVESVGSDGGTATVRLHFTD
                     LISPLIRLEVYAVEGGPRSFSETELHSAYREVRFLSVRQRLEPPQMELGASLPAPSEY
                     GDLSLLKMLAQEGVRTVHAPAGESTIIRVSEKGLTLLPELEHISVNEALDGKYRVPCL
                     NDPSYRISLEETLKTGTLRHWAGTRGRYSLGNGNVLCATEEAVCRCGYCLEAWHQVLR
                     EKYTSLEALNRQWGTHFSEWEQIDTVDVPPVNEQTRTVSYQVDFSTFMDSQFAGFHQW
                     LHEQIRLIDGSAVAGARFFSDTNPSHGYCWPELCRALDFVIADYSPLILEKIASYTAP
                     GNWNGLVLHPSDAGQNGAAFLQIPWQMLTHQIPVLWLEALSGEAEKADGNTWFDVQGS
                     LSEALTAITRTVRQLQDTIAPLILASEKKPPAIAVYDSHPSRHLPKSASGYNTTFLDS
                     QNAVSACLRRSNRDFVFIDKERLDGLRQDTFSTLILPFCVALDDEETEAIFTFVARGG
                     TVAADVLPGNFDTHGGKRLNNRFASLFGVDQKPDSRQCTELLVTTEDVEGRTRVSGQV
                     EADAGITPDRAVALAKAGDLPVWFISRWEQGNTLLLNHPFRMPVGDFLAEMACLNEFI
                     TDSSASSPQQVPTDPGFQGMVYHFRYDSAVIYVILAAPSAPVQPLPLHFTKKDSVYLP
                     LTGTRVAHPHRLKIRLNGGESLALVHLPKDAKPLKIHSTETAAEGQRLEIRLTSDGAE
                     EDPVKRLILLDLLGADQTVIPWYRHVAVLEKGHGASYFPLSLNQQPGRYVVRARDLLT
                     GAVVEKPVKVTSIIN"
     gene            complement(20842..22182)
                     /locus_tag="PKN92_04025"
     CDS             complement(20842..22182)
                     /locus_tag="PKN92_04025"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ47993.1"
                     /translation="MKKNEKKIISRRSFLNNAGLAMAAPLIVPSTVFGAKAHKSPGSK
                     ITIGVIGSGGQANGLTENAIKHKNTRIVAVCDVDRRRLAEAKSKVDHYYDNEDCTVYT
                     DYREVLAREDIDAVIVATPDHWHALICVEAAHRGKDIYCEKPLTWSLGEGQAVVKAVT
                     ENKRVFQTGSMQRSGKDFKTACELVRNGYLGKIKHILVALPNGGNALWVDEFPPPPAE
                     LDWEFYVGPAEWAPYHEKRCHWDWRWWMGFGGSQMMDWIGHHGDIAHMAMNWDHTGPV
                     HVQGVAWEMSPERNNLYNAPQKYQFECTYADGTTMTVGSSDKMPDLFKECGNLGTMFF
                     GDNDQWLYVDRSGIKASDPELLQTRLGDTAFRFRKGTNHMTDFLQCIESREECIAPVY
                     AGHRSASIGHLGKIACTLNVGFNWDPEKEVITDNPAMNALLTRQYRGGWSLDAV"
     gene            22515..23702
                     /gene="metK"
                     /locus_tag="PKN92_04030"
     CDS             22515..23702
                     /gene="metK"
                     /locus_tag="PKN92_04030"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNZ47994.1"
                     /translation="MSKLLSSHVFTSESVSEGHPDKVCDQISDAILDACLAADENSHV
                     GCETLAATDFVANAGEISCEGWSSIDPESLARAVVKDIGYIYDDIGFSYKTFQYVNRL
                     HQQSADIAMGVNTNEETHKEQGAGDQGMMFGYACDDTPQLMPAPLQYSHNLLIELARI
                     RKAGEIAYLRPDSKSQVSILYENGKPAKITTVVISHQTEDVPLEQIRKDMIDVAQQVL
                     GATGLLDNDTKYHVNPTGRFVIGGPHGDSGLTGRKIIVDSYGGVGSHGGGAFSGKDPT
                     KVDRSAAYYSRYAAKNIVAAGLARKCEIQVAYAIGVAEPMSINVSTFGTGKVEDTVLQ
                     RVLENSGLFDFRPAQIMAELSLAKPQGWCYRDTSNYGHFGRDLFPWEKTDKADALRQA
                     VGK"
     gene            23699..24706
                     /locus_tag="PKN92_04035"
     CDS             23699..24706
                     /locus_tag="PKN92_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNZ47995.1"
                     /translation="MTDTPTASRILGVGSPIMDILAPVPDSFLEKISGAKGGMEWIKH
                     DEMDALLAQMPEPPQRALGGSAANTVAALGRLGMPVALLGKIGRDADGDAYRAAFTAS
                     GGVEDSFKYDDSSPTGRCLSLITPDGERTMRTDLGAAMLIGKSDVSDRDFQQCRHVHL
                     EGYGLFNCEYVFHLLQMAKAAGCTVSIDLASFEVVRDSMDVLPDLLKHYVEIVFANED
                     EAAAFSGNPDPEEGLRALHACSPVAAVKLGKDGALIRTEDACSKVDAVPVARVIDTTG
                     AGDCWAAGFLYGYLNGWSPEMCGEFASHVGAETVRSLGASPDAQGWTRIRQAEATLKN
                     R"
     gene            24759..25406
                     /locus_tag="PKN92_04040"
     CDS             24759..25406
                     /locus_tag="PKN92_04040"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HNZ47996.1"
                     /translation="MMYVGRIVSVGKTADGQLCAAYRVSSRSFPNRSAVVGNGKVSIV
                     PKPGFESDIFKNPYIAYNCVRIVCDGAVAVVTNGGQTDFIAEKMAGGMSARDALILTS
                     FSMDYEKDDYNTPRISAVVDKRSDEGWLAVVRHDGLQVERIALKNGFQWHLATYEENF
                     ICPERFDAFPATSAESACAYMLSGGVFAERTNPVTAVAALAEESGFALAVQDFQA"
     gene            25492..26922
                     /gene="purB"
                     /locus_tag="PKN92_04045"
     CDS             25492..26922
                     /gene="purB"
                     /locus_tag="PKN92_04045"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNZ47997.1"
                     /translation="MSDLYVSPLVERFATDPMKRLWSAQKKFSTWRRCWIALAEAEQE
                     LGLDISDAQLEEMRAHVDTIDFEQAAAYEKETRHDVMAHIHAFGDLAPNARPIIHLGA
                     TSCFVGDNTDLILIREGLELILAKAAGVLNRIKTFALQYKSLPTLGFTHYQPAQLVTV
                     GKRACLWAQDLLLDCLQIEQLLASMRCRGVKGATGTQASFLALFHGDAEKVKALDHLV
                     AKKLGFESSFIIAGQTYTRKIDTQVLNALAGIGESAHKFATDMRLLQNLKEVEEPFER
                     SQVGSSAMPYKRNPMRSERICALSRFLMTAPLHSAFTTATQWFERTLDDSAIRRLSLP
                     EAFLTADAILNLYLNVMEAPRVYPKVIEKRIWSELPFMASENILMACVQKGGDRQTLH
                     EVIRQHAQEAGRRVKEEGADNDLLDRLAADPQINMTREEMEEQLKLDAFVGRAPSQVD
                     EFFVQEANPFLERHTASLDTHSDVRV"
     gene            complement(26951..>27539)
                     /locus_tag="PKN92_04050"
     CDS             complement(26951..>27539)
                     /locus_tag="PKN92_04050"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNZ47998.1"
                     /translation="ADLMSWVSIGARTSESQTHREMASGLSMPVGFKNNTSGNLQSAI
                     DALIAAGRPHHFLGVDGAGRSCVVHTRGNSYGHVILRGGRSGPNYDPVSVITVEDQMR
                     SAGLEPRIIVDCSHANCGKRPRLQAHVLRDVIQQRLEGNKSIYGVMLESNLQEGNQKL
                     SGNASDLRYGCSVTDPCIDWEMTEQLLREAHRKLA"
BASE COUNT         6882 a         7002 c         6855 g         6800 t
ORIGIN      
        1 caggctgcgc cgggtttcga ccgaatctgt gctcaaagag ctgattcagg aaattcctgt
       61 tcctcaggaa tcaactagga ctcttaatgc atagctgtag tggtagattt gcgaatcctt
      121 ttgatgttac actatatgtt gttattccac tatttaatga ttgtttttaa aaagcagtcg
      181 atggagtcat gagtcatggg gattatgtca aaactcggtc tgcataaagg gtctgcaaaa
      241 acggcttctc cgggccagtt gggtggcgct gcaggtttgc acagcagcga atttgagctg
      301 tcccacgtgg gcccctatca aatacaggca cccatcggca gcggcggtat gggcactgtt
      361 tataaagccc tggatcccgc ccgcgacgtg acagttgcca taaaagtact tgaccgtcgc
      421 tatgaccttg acaagaaaaa aagtaaacag gactatctcg gacgggaagt tcttattgcg
      481 gcatctctca atcatcctac cgtcattaaa atggaaaagg agatcattaa tcaggaagat
      541 gttgagggca atctgcgccg ctgcctgata atggaattca ttgacgggta caatttaaag
      601 cattttatcg acaaccggat cctgacaata aaacaaatga ccaacctgac aatccgtctg
      661 gcggaaggtc ttgactttct gcaccagaac ggtgtggtgc acagggatat aaagcccgcc
      721 aactttttga tctccagaga tatgagtcag gtgaaaatat gcgacttcgg gctttcaaaa
      781 tcaagtgcaa cctggcgcac ccgctggctg aaagagaaag gcggaacacg tctttacatg
      841 tctccagagc agctgagaaa gcaaacgctt gatgcccgtt cagacatttt ctctttcggc
      901 atcaccatgt ttgaactttt tacaggccgg cacccctgtg cgggcaacaa tccgcgggaa
      961 attcaaaaac aattattaag ttcgaagtac agattcccgg caccttcctc tattaacaga
     1021 gaagtgcccg agcggatgga tcgggttatt ttaaaagcgc tgcgccgcaa tcttgacaca
     1081 cgctatcaat cctgtacgga aatgttgctg gacattacac gcgtgtccgg ttcaaggatc
     1141 tgacttttat gaaaaaacgg attttcagta aaaaatcacg ttgggaaccc ggacttttta
     1201 ttatcgctct ggtcgttgct gccgtcatgg ctgtgattat tgccgcccct tatctcctct
     1261 ctaaaacaaa agaggccttt cctgtagtcc tgccagtaag cagtgacgga ctggaagtcc
     1321 tgcagcaggc gcaaaaaaat gtggcatccg gcaactatga cgaagcaatg gcacaaataa
     1381 aaccgcttct taaaggacgg aatcctctga caacaccccg agccattatt ctggaagcag
     1441 atattctgca ctacaagaac cagccggaaa cagcgctggc aaatcttacc gcagcttgtg
     1501 atgcttttaa aagcagtcct gaatatccct acctgcaggc aagaaaagca cggcaacttg
     1561 aagaaatgag ccgaacagca gaagcccggg atatctacga atccatccga gatcatgctc
     1621 ctgcagaaat ccgttcgctc ggctatctgg gtctggcgcg acttgatcag cgggcgggaa
     1681 acctcgttgg agcaagagat ctggcacggc gtgctgctga agactcacca tggggcactg
     1741 tcgtatggaa cgaagcgatc gacatgctgg ggccgctgaa tgtggcactc attttttcgc
     1801 cccaggaaac cccggaaagt aagatttaca cggtagaaaa aggggataat ctcaacagta
     1861 taggtattaa actgaatacc acacaggggc tcttaatgga agccaattcc attacatccc
     1921 cagaaaaact gtctctcgga cagcgtttaa aatatacacc aaaagatttt tccatcatta
     1981 ttgaaagaag tacctgctct ctttacctga tggataataa aggtattttc aagcgctata
     2041 aaaccggcct gggcaaacca ggacatgaga cgactttggg aaaatatacg attggaacca
     2101 agcagaaaga ccccgtttgg cataagcccg gggcgggtcc tattgcagcc aatgcccctg
     2161 ataatgagtt gggtacccga tggatgcctc ttgaaccggc agcagaaggc cttcccagag
     2221 atctcgggat acacggaaca atcgctccgg aaacaatagg ctcttttgcg tccaaaggtt
     2281 gtgcgcgtat gctgaatgaa gatgtggaag aactgtacga tctcgtggta cgcgccacgc
     2341 ccgttgaaat cgtggagact ttggatccca acacacagca ggcaccaacg gttgcctcct
     2401 caacccctgc cgcagcagaa gcggtgcaga ctgctgcaga atagactgca cagactgcac
     2461 accagccccg gtcctctttg acaagccttg agattttact atgcaccttc acgaaaaaaa
     2521 tatagctgtt gttatgctgc acgggtcgtg cgatatgttt tgcaaatttt gcattacaga
     2581 taatgcagtt gaatgcatga caccggatga tttcgctgaa acactgactg tactagaaaa
     2641 agcaggattt acaaatattg taatcggtgg cggcgagccc tttacctggc cctacggact
     2701 tcacaatgcg gcaaaaattg caaaaagcaa aggctttctt gttcaggtgg gcaccaacgg
     2761 aattaaaatg cctgatggcg ctgcctaccc cgactgtgtt gaccgttatg tgcttcctct
     2821 ggatggtacc acccggacag agcacaaccg tgtacgccat tttcctcatc agatcggggg
     2881 gcattttcaa attattatga accgccttgc ccaattgaaa gaagcagaac ggtctgtcac
     2941 tgtatccact gttgttacac gtcataccgt tgccaatctg cataccctcg gtgcgtttct
     3001 tgcggattat gtacagacag gcggaatact tcatgcctgg catctttacc gttttcttcc
     3061 ggcgggcagg ggaggggcaa agtcggctga agaattgtcg atcagctctg ccgaatttga
     3121 agatgcaacg acggctgtga aagatcaaca ctatcccttc accattttta aacgccgtga
     3181 catgcgtcat tctgcgcacg tagatttctt ttggcggcag aaaggtactg tcctccgcgg
     3241 aagcgactac tggaagcaga cagctgcgct gtcaccgatt ggtgccttct gatctttccg
     3301 gaaaaattgc atttggaata agaacaggca tattattttc taaaatgatg ttagttgaat
     3361 cgtgagctgt gtttttgcca acaatgcaga aacttagggg tatgctggag acttttttat
     3421 gagcgatgaa ctgtatggaa cagtaacaat tgacgaagat gccattcaaa atgcattgga
     3481 tcattccagc gaggatcatg cccatattcg ggaagtactt gccaaggccc ttgagttgaa
     3541 ggggcttaat gcggatgatg tttccgctct gatgcgcatt cagtcccaag agctggaaga
     3601 agaacttttt gaaactgccc gcaaagcaaa aatggaaata tacggacggc gcctggtttt
     3661 atttgcccct ctgtatgtgt ccaatctttg tgccaatgac tgcacctact gcgcgtttcg
     3721 atcaaaaaat aaagccgtaa aacgccgcgc tctgtctcag gaagaagtag cggaagaaac
     3781 gcgtatcatc atcaatcagg ggcataaacg cattctgctc gttgccgggg agtcctatcc
     3841 gcgggaaggt atcgaatacg cctttaagtg tatcgatact atctataaca cgaaatcaga
     3901 gtggggagaa gtgcgccgca tcaatgtcaa cctggcacct cttactcttg atgaatttca
     3961 ggggcttaaa gaccggcgca taggcaccta ccagctcttt caggaaacct accatcggga
     4021 cacgtacagc aaagtacacc tctcagggaa aaaacgggac tttgactgga gaatcacggc
     4081 gatggatcgt gccatgctcg ccggcatcga tgatgtcgga atcggcgtgc ttttcggact
     4141 gtatgactgg cgctttgaat tgctggcttt acttcagcat aacacccatc tggaagagcg
     4201 atttggcgta ggctgtcata ccatcagcgt accccgcatt gaaccggctg tgggctctga
     4261 tctgtcagaa caccctcctg ttcctgtgag cgacaaggat ttcagaaaac tgattgccat
     4321 tcttcgttta accgtgcctt ataccgggat cattctttcc acccgtgaaa atccggaaat
     4381 gcggcgggaa gcgctggctt taggtgtatc gcagatttct gcgggaagcc gcaccaatcc
     4441 gggcgggtat cacgaggaaa gctatgatat gagccagttt tctctcggag atcaccggtc
     4501 actggatgaa gtggtagaag atatcgccca ccttggatat atcccttcct tctgcacagg
     4561 ctgctaccgt ctgggcagaa ccggcaaaga ctttatggat ctcgctaagc cggggctcat
     4621 aaaacattat tgtgaaatca atgccctgag cactttcgca gaatttctgg agcacttcgc
     4681 atcaccggct acacaagaag tcgggaatga acttattgac cggattatag aagaacaacc
     4741 gcccactcga aaagaacggg cgagcaccgt tgtcaggaaa gtacgtgccg gcgaccggga
     4801 tgtttatgta taataagcag gataagcgca agttgtcgct ttaaccagtt tactttccgc
     4861 caaagggtca tgggaggttt tatgaaagat cgacacatta tttacggtgt acacgtccag
     4921 aaccgcgggg aaaatgccgt gcgtgtgcag cagctgctga cagagtacgg atgttttatt
     4981 aaaacgcgcc tcggtctgca cgaggtggac gagcaattct gtgcaacagg aggcattctg
     5041 ctgctcgaaa tgtttggtga catcgcccgg tgtgaagcac ttcgcgacaa gctgaacagc
     5101 attgagggca tagaagtgca ggaaatgatt tttgatcaca gctgagcgct gttatcttgc
     5161 ttccggacac actgaaacgg tcattttaaa gttacgacca aagcaagaga gcgtgtaatt
     5221 ttccggacag cctcctcctg tgaagaggct gaagaggtgc cccttttcag gcggcggaag
     5281 gctgcaggtt cctctctcag aaaagatagt accgcttcca tttttacggc gtagtttatc
     5341 ttttgtgcaa tagatcctgt tgcctcagca taataaccat cgttcacccg ggcgctgatc
     5401 acaccgacca cctctcctga ggtaaagaac aaggggcctc ctgaattgcc cggctggaca
     5461 gcagcatcga tctgatagtt gttttcgtca tcatgcatac cagtgagagc gctgataatt
     5521 cccatggtga ccttcggagc aaacccttga atctccggat tggggtaccc caaagcaaat
     5581 accgttttcc ccggctgtac ctcgtcagga atccctatag taacaggcgt gaaggtaccg
     5641 tccactttaa gcagcgcaag atccttttcc ttatcggtgt gaataatctt tgcggcatgc
     5701 acttgctcgt ctggcaacaa tactgcaacg gcaccggcat cttcgaccac atgcctgcag
     5761 gtaagcagat agccgttttc agtcacaaag aagcctgttc cactcccggt tatctccaaa
     5821 tcatcttctt ccgaatcgtc gtcggcgtct tcttcgtctt cctcatcgaa gagatcaaaa
     5881 tttattcgaa ccaggctcac tgccaaagca cggtcggaag agtaacggtg aacggttttt
     5941 ggcatatctt ttactgttgt gtagctgtaa gtaccgcacc agtaaaggtc tttcagaaaa
     6001 gtctcatcgt ctgctgtata acggcttgtt gcgtcgatcc aaaagaagac gctcccagta
     6061 aaatagattt cttcatcctc ttcatcgtag accccttcga tgcacagaca gccgtcctcc
     6121 atggcctgaa gcactttaaa gacaacattc ttttgtgatt tggaaagcat aatctcttct
     6181 gctctccgaa tatagttttt ttcgcggtcg gacatggggc taagcgaact gcttttgtct
     6241 gaattctttt ttaaagtgcg tgcagatgac tctgcgcctg catacatgcc gcccaacaat
     6301 aaaagtaaaa aaataacaaa gccagtacac gtactgtgtg ctttcataaa ggtgccctcc
     6361 tttatttctt cactgttatc cagtttctca aaagcaatgt ctccataaaa ttacatatac
     6421 cggatttata atacaaatct cagatgttaa agtcccacac ccagacaggt gaattcaaag
     6481 cctgcctgaa cccagctgtc ccgaggcagc gcccggcggg tatcaaacac tttccggttt
     6541 ctcatccatt cggaggcctg caccggcgaa acagtcttaa attcgctgtg atctgtcaga
     6601 agaaggatca tatccgctcc ttgaaggcag ctctccagac tttgcacagg gtatcggtct
     6661 gtctttacaa aaggatcaca cagccgaagg tcaaccctgg cctgctgaag aaactctgtc
     6721 acttttaaag cagggctttc ccgcaaatcg tccacatccg ctttataggc cagacccaga
     6781 acaactataa ccggtttttc tatgctagcg gtcatggcaa gcgctttttc agccactttc
     6841 cgaggcatat catcgtttct tcgccgtgcc tcctgaatca tggaagtccc ttcaggattg
     6901 gcctccacaa gaaaccaggg atcgacagcg atacaatgcc cgcctacacc gggaccgggg
     6961 ctgtgcagat gtacccgcgg atgatggttg gcacagcgtg ccatttccca ataatccaca
     7021 cccaattcct cacaaagaag tgccgtttca tttgccagtg caatatttac gtctctgtag
     7081 gtattttcca ttaatttgac cacctccgct gtcgttgccg tggtggcaat gagctttcct
     7141 cgcacgaagc ggtcataaag agcaatggct ttttttgtgc tggattcatc cattccgccg
     7201 attacccggt cattctctac aagctccgta agaatttttc caggaagaac ccgttcaggg
     7261 caatatgcca gaaagaggtc ggtgccaatc ctaaaaccgg cactctcaaa aagcggagca
     7321 agtaaatcac ggcatgtacc cgggggagaa gtggactcga gtacaaccag attgcccggc
     7381 cgcaggcacg aaataatgct ctgtgcggca gcgcgcacgc aggacatatc cgctttgcat
     7441 tctgcagtaa taggagtcgg caccgcaatg ataaaaacgt ctgcaggcac aggacttccg
     7501 gtggcacgca gctttcctga ggacaccgct tcctgaaccc atcctgacag cccgggttcc
     7561 tctatatgaa ttgcgccctg gttgatcgtt tttacgacat cagggttcag gtcgactccg
     7621 attacttcga ggtcgtgggc cgacagtaca gccgctgtgg gcagaccgat atagccgaga
     7681 cccagcacac acactttatt cattatcatt gtcctgtttt tgcgttcagt gcagcaggaa
     7741 tcagcagggg tagtcagata ctgcaggcac ttttagaaga aaaggtttca cgtcattttg
     7801 tattgttgcc gcaacagcct tataataccc attgatccgg aaataaaaca acagatagac
     7861 ctcctttctg tacattttcc caacaagcgc aaataaacct gtccaccatc cctttttttg
     7921 gagaaaccta ttcatgaaca aaatcgctga actttttgca aaagccggat ccattcagga
     7981 atatgcaaaa ttgtataacg cccgtttgtc tgaagttctt gcctcacttg actgggatag
     8041 cttcgccaaa gcagtgaccg cttttgaaga ggcacgtcag tcaggcaaag ccatttatgt
     8101 tatgggtaat ggcggcagcg ctgcttcctg tgcgcatctg gtcaatgatt tgattgccgg
     8161 tgcctatctg gatggaaaac ctgctttccg cgctgtttct ctggccgaca atctggcaac
     8221 tgtcaccgct cttgccaatg acagcggtta tgagagtatt tttgtccacc aattgaaagt
     8281 gctgcttcgc cccggcgatc tggtacttgc catgtctgtc agcggcaaca gtcccaatat
     8341 tttgcgcgct cttgagtatg cgaaatcgga aaaagccgtc acagtcggct tttgcggttt
     8401 aaccggcggt gccatgaaaa atctctgcga cgttgtgatt cacgccgata ccactctgga
     8461 tgaatacggt ccggtcgagg atgttttttc cattctgggc catattcttg ccggctatct
     8521 ttccatgaaa caggggaaaa tgctgcatca ctgatgccca ttgaagggga gcaggaaaac
     8581 cagtcccttt gcatgcttct attatcatag cggtctataa tcgtcgcgag gacgtgcatg
     8641 cgtgcctgag cgcactgccc gttgctgagc tgacgcaaaa aaacactgaa gttattctcg
     8701 ttgatgacgg ctctaccgat ggcgtcaggg agatggcggc gcaggaattt ccccatgtgc
     8761 agacgctcag actggaagac aacaaaggcc catcctttgc acgcaatctg ggtgcgaagg
     8821 tggctcgggg ctctctgttg atttttctgg attcggacgc cgtcccggaa gacaggtggc
     8881 tggatacgtt tttgctctgt gacgacggga acactgttct cataggaaga attctggatt
     8941 accacacagg gaaagatcag gacagaccga ggcgctccac ttttcttgga aaaagccttc
     9001 cctgcagtcc ccggcacgcc aatgccggtt cttcctgcaa tcttgcactg ccggcatcgc
     9061 ttttttctgc aatcggcggt tttgatcctg atatcccgta ctattttgag gacagtcttc
     9121 tctgcattca tgcgcggcgg catggagccc gtttccgcta cctttccgat gcacgggtac
     9181 gccataaagg cagcggcacc cggcagggcg ataccatacg catgcaggaa cacaacagca
     9241 cttacgccat gctcatgctt tacaggcact ctttcggacg agtcctgctc tttactgccg
     9301 gcaatggtct ctggctgcta atccggctgt ttgtccggct cctgaccggg cggcttcatg
     9361 atgccgtgct gctggcaaaa gggtggggaa gtgcttattc acgctttgtc agccggacag
     9421 gcgactgacc atttctttga tgcctccgag actcatatcc accataggga aaattctgcc
     9481 aatattttca atggtatagg caccgtgtgt ccgcatccag tattcccggg gtatgggatt
     9541 aagccagcag gaccatggga aacggcgtga tagcttgtgc agccaggtac tgctgggcgg
     9601 gctgctgaag tcactgaaat tggagagagc accgccgcgc gcttccagct cttccggcgc
     9661 catggatgca tctcccacaa tcacaagtct cgtatccggt cgcctcaata gcagttgttc
     9721 cgtatctaaa gggctggtac ggcgaaaatc tgtccacact ttttcataaa tggtattgtg
     9781 gaaaaagagg cattgcagat cttcaaaccg ctcatgcatt ttagaaaata gaatacgggt
     9841 gatttcgata tatggcagca tggaatatcc gccgttgtca atcagcagaa gcacactgat
     9901 tttatcgcgc agatcccgct caaaaacaag atctatctca ccgccgttcc gggcagtcat
     9961 cgtaacggtt ttatccaaat tcaggcgcgt aaagggtccc gcatgctgca tgtgtttcat
    10021 ggatgccagt gcctgccgga ggttttcctc acgcagctga ttcctatcgg aatactcgac
    10081 gtaacgacgg tcaccgatca cacgcaaggc agaacgattt tttgaagcgc ccattacccg
    10141 tattccccgt gaggcattcc cgctgttacc gaaaggggag ttgcctcctg tccctatcca
    10201 tcggcttccg ccgtgatgct cttccatctg ctgtttaagc gtgtcccaga accgtttcat
    10261 caactcttcg ggctcataat gccagaaggc atgcctcggc atacgccctg tgcggatttc
    10321 ttcttccagc caccgccgga attgctctgt gctgaatagc gcatcgtcgc cttcctgtac
    10381 ggcgggaata tcgatatcat aaaagtacaa ggcaaaagcg cgttcaaagg catcggcata
    10441 ttcaacccgt tttacaaaaa ccagcctcga aaaaatgaac aggtcgtcca gcgtcgatac
    10501 aatccctttt tgcagcccct gattcaattc cagcagttca cgcagcccaa ccggcagccc
    10561 gtaatcacga aggagataca gaaaaggtgc aaagagcact gtatagcctt tgctgttatc
    10621 atctctgttt gagggcatcg ccatatacta atgcctgccg aatctccgct gccggtattt
    10681 cagaatatca gcactgcgtt ttaaaagaag tccggttaga ggcgggaatt caggatcagg
    10741 catttttccg tcccgctcaa gtgcgccaat ccagtcaatc agttcgcttg tggctggcgg
    10801 tttttcaaaa ccccgatccc gaagctcata aaatatttta agcgctgtct caatcagctc
    10861 gggatgcgta ttgggataat gcagcgcaag aatggcacgc atttcctcag gagctggaaa
    10921 agcgatatgg tgacagaaac atctgcggag aaaaggatcg gacaattctc ttttagcatt
    10981 ggatgtgata aagattgccg gtcggatctg cgccttcact gtttcgttca cctcacggat
    11041 agtgaactgg cattcttcaa gaatgtccag cagattgtcc tgcacgtccg actctgtttt
    11101 gtcaatttca tccagcagaa gcactacttt tctgtcggcg cgaaaggccc ttcctatagg
    11161 gccccagatc acatagtcat aaaactggtc aacccgcctt ccgctttctc ctgaaccgaa
    11221 gcgggcatca ttcaaccgca gcactgtatc ctgtacgtaa caggcttctt ctcctttaat
    11281 cgtggatttg cagcgtagga tttcgacagg gaggccgaga gccctggcgg tttccagcgc
    11341 cagctgcgtc tttcctgttc ctgcttcacc ggtcaacaga agaggttttt ccatggcaag
    11401 cgcaatattt accactgcct ttagttctgc atccagatga tagcggtccg ttccgtcaaa
    11461 atgatatgaa ttcacgagca tcctctccgg agaagtttgc tttttacagc tgaattgcct
    11521 agcatagctt tttcatatat aagttcagga tcactattat ggcaaattgg cttagctaca
    11581 ccagtacaga aggagcgtcg ccacgcctcg ctgctgtaat catactgtac gaacaacagc
    11641 gtcaacgtgt tttattcgta aaacgaaacc catcgcttcc ttttatgggc gggcatcacg
    11701 cctttccggg ggggagccta tcagatgctg acacaggcag ccgggttatc aatgcgccag
    11761 acctcaggag cgcgcgcctg ctcaccacag ctgtgcggga actttttgaa gaaacaggca
    11821 tactgcttcc cgatttgacg gaagcggaaa acggatcgct gcaaagcctg cgtgagaaaa
    11881 ctgtcagcga gcccgccgtt tttgaagcct ttctcgaaaa aaagaacata tttattgact
    11941 acctgaattt ttctcctgca ggccgatggg tgacgccctc tttttcacct atccgtttcg
    12001 atacttctta ttttttctgc agcacctcga agccctgttt tgctgcgccc atgggagcgc
    12061 atgctgaaat agtcggcgtc gaatggatca cccctgccga agcactcaaa cgccgggacg
    12121 gaaagagcat gcacgtctcc acaccggtag tttttgtttt gcaaaggctg catacctttc
    12181 cgcttccgga agcattaaag cgcctgcgtc acacacctgg tttcagcaac accctgctgg
    12241 attacattga gccatttccc ggcattcatc tggtaccgct gcagagctgc accttgccgc
    12301 ccgctaccca tacaaactgt gtgctcatcg gcgaagagtc catttatatc gttgatcccg
    12361 gtgcttcaga aacatctgag atttcccgtc tttttacgca tatagacgaa gtctgcgaaa
    12421 acgttggtgg aacacctgcg ggcattttgc tgacccacga tcatcccgac cattgtgccg
    12481 cagcagaagc tctttcgaaa cgctacaacg tcacagtgta cggacatccg gcgtctctcg
    12541 gctcatttca aaacgcagaa gcactggaga agggagactt tattgaaatt ccgggctccc
    12601 gcccctggtc tgttcgatgt cttcataccc ccggtcatca tccaggccat ctctgttatt
    12661 tcgaagaaac aaccggagcg ctcctctgcg gtgacatgat tgcgaacccg ggaaccatta
    12721 tgattaatcc ggataagcat ggagatatgg ggctctacat ggcgggactt gaacgtctgg
    12781 gcaaaatccc ggccgcttta actgtgcccg gtcatggcgc cccgctcatg gagggggaag
    12841 gaccggctct ttttcagaaa actctggaac accggataat gcgcgaaaca aaaattaaaa
    12901 cagcctggga gcagggtgcc cggtgtgaag atacccttct ggcacaagcc tatgatgata
    12961 caccggagtc catgcatccc tttgctttgc tgcagctgcg ggcgcatttg agccacttgc
    13021 aggagacaga ccttatttct ttctgaagta atctgcctgc cttttctttt attgatgaac
    13081 gcaagttcgt gagacgattt ttttccgaca ggtatgctat actttactgt aacgaatcgg
    13141 agcaaacaat gaaaactcag aatactaaaa cacataaaga atatactgta ctggaagaaa
    13201 ttatgaacag cggcacccat gccgttggtt tcggattgag tgttgccggg ctggtttggc
    13261 tgattctgcg ggcggtatca cttaaagaca gtatgagcct ggttgctgtt attgttttcg
    13321 gcagttcgct gattgcactc tatgcggcat cgaccctcta tcatgctgtg ccctgtttca
    13381 atgtgaaaaa agtcctgcgg gtattcgacc attgtgccat ctatctgctg attgccggca
    13441 cctacacacc ctttctgctg gtgtcgctcc gaggaaccac tggctgggcg ctttttggag
    13501 tcgtatgggg gattgcctgt gtcggctgtg ccttcaaagc cttcttcacg ggacgcttca
    13561 ataaagtctc tacagccatg tacgttgcca tgggctggat cgttctggtc gccataaaac
    13621 ccgccattac ccatatacct gtcggcggtt tactgatgat gcttgcaggc gggctgcttt
    13681 atacatttgg cgtctttttc tatgccaatg agcgcgttcc ttataatcac gcaatttggc
    13741 actgcttcgt tatgggtggc agcgccattc actttttcgc cgtaattatt tacgtactca
    13801 cgcccatagc ataaaaaaga caccgcctag cgcagcgcag atgctgcaag aagggcgggc
    13861 acttttgcgg cctccttctg tgcctcccgc tgtttcttcc actgttcata ttttgcggga
    13921 tagtcctcga gaacccactg tttaaaaggc ttggggctcc cttcgtaaag cgttaccagt
    13981 tcgccgatgg gtgcatcgtt aaaaccgatc cggaactgct ttcgtgctgt tgctgtatct
    14041 gcgcctttca aaagaagata aacggcggcg gcaacaccgg ttctatgcgt tcctccctgg
    14101 cagtgagcta gaaaaggctt ttgccctgtt tccatgagat caagaaaagc ggcaagcgat
    14161 tccggttccg gaatgctgtt tttcgaccat ccgaaattaa catgctgaat ccccatatgc
    14221 ttgcatgcgg ctacttcctg atcgtaccag tcactatccg tttgctcgcc tcgaagattg
    14281 atgacagtgc ctatacctct tttcttaata attcgctcca ttgcttttcc tcccatctga
    14341 cgagacccat aaaacgcccc ttcttcgacg gttcggaaat tgtacatggg aaagtaatga
    14401 atcaccggca ctgcaagaac cagagcggca ataagaacaa cagcaccgat tatctgagca
    14461 atcgttttca tggaattcct ttttggatct gtgtttagag aaaagaggaa agaattttct
    14521 ttgaatgcta aatttttttg cctgatattt tgtttacaca cctggaaggg cgtaccggaa
    14581 agcaaaaact tctgaagccc tgttttgaag caacacggtt ttattttatt atggtaccat
    14641 aagcttttaa acaggtccaa ttcaccgaaa aacacaaatc tgcaactgcg acagaatttg
    14701 tttctgtctg attgagatga gctcatgcga agcaccccta aatcgatgcg tcttcatatc
    14761 ggtcttttcg gaaggcgcaa tgcaggaaaa tccagtctgc tcaatgctgt aacggcacag
    14821 caggtgtcca ttgtttctcc tgtggccggc accactacgg atccggtaga aaaagcgatg
    14881 gagttgctgc cacttgggcc ggtccttttt atcgacacgg cagggctcga cgacgagggc
    14941 accctgggcg aactcaggaa agaaagaacc agggctgttt tcgaacgggt cgatctcggc
    15001 gttatcgttt gccaggaagg tgagtggggc gcttatgaag agatgctctt ttcagaattg
    15061 gaccagcgga aagtacccgt tattgccgtt tttaataaat cagatataaa acgcccggat
    15121 acggcacttc tgaacatgct ttctgcaaga aaaattgctt cgctctgcct cagcactatt
    15181 accggagaag gactgggtga cttcaggcag gccttattgg atagtgcacc agcggatttt
    15241 gtcgagcaac cggtgatcct ccgtgacctg gtgggcgccg gtgagctggc agtactggta
    15301 acacccatcg ataaagaagc gcctaaaggg cgattgatta tgcctcaggt gcaaagcatc
    15361 cgggaccttc tggacggaga tgcgtcctgc ctggtgacaa cagacaaggg gcttgcggct
    15421 acactcgcca aattaaaaga gccgcctaaa ctggtagtaa cggattcgca ggcgttttta
    15481 aaagtagcgg cagatacccc ttctcatatt ccaatgacgg gtttttcgat tcttttttca
    15541 agatatcgcg gcgatctggt tactcaggtg gaaggcgctc tggctattga cagtctcaaa
    15601 ccgggcgacc gggtgctgat tgcagaatca tgcagccatc atccgataga agatgacata
    15661 ggccgtgtta aaattccaca gtggcttatg aaatatgttc aaggcgaact tatctttgat
    15721 tcggtacagg gacatgactt caaaatggat ctcagccctt acaaactggt tatccattgt
    15781 ggcgcttgcg tcaccaatcg ccgtgccatg ctgaaccggc ttctccgttg caggaaagca
    15841 ggtattcctg ttactaatta cggattgact attgcgtaca gtctcggtat ctttgaacgg
    15901 gctcttgagc cttttcctga tgcactggcg gcctatgaga agcacaaatt gtatttgaag
    15961 gcaaaaaaca ctgaagcgta agccgacggt ctatcttcag caaatgctct acaaacacag
    16021 gatgaacaag ttttatgtct gattttctcc aacataaaag tcaggaagaa atcgaaggat
    16081 ggctccgcga aacagatccg gagcgtttaa aactgttatg ggctgcggcg gacgagaccc
    16141 ggcgccgcca tgtgggagat caggtacacc ttcgcggtct gatcgaaatt tccaactact
    16201 gtatccgtgc ctgcgcctat tgcggactgc gcgcaggaca tggcagtgta gagcgctacc
    16261 gcctcaccag ggacgaaatt ctttcctgtg cgcacaaggc tgtagggttt gaatatggaa
    16321 ccgtggtact tcaggcagga gaagactacg gtatcaagcg cgactggctg gctgaaatca
    16381 taaaaaccat taaaaaggaa actaacctcg ctgtcaccct gagtctgggg gaacgcccgg
    16441 acgaagacct gatcgagtgg cgcactgccg gagcagatcg ctatcttctt cgttttgaaa
    16501 cttccgacag agcgctttac gagcgtattc atccgcctgc gaaaggacag gagatcagtg
    16561 acagacctgc tatcctgaga cgccttaagc aattagggta tgaagcgggc agtggtgtaa
    16621 tggtaggtat tcccggtcag aattattcca tccttgcccg ggatattctg ctgtttcaag
    16681 atctcgatct tgatatgatc ggcgtcggcc cgtttatagc ccatcccgaa actcctctgg
    16741 gacgtggcga actggaatcg gctcctgatg gagagcaggt gcccaatacg gaaggcatga
    16801 cctataaaac agtggcgttg acccggttga tatgccccga agccaatata cccagcacga
    16861 ccgctctcgc caccattaac acggcgctgg gcagggaact ggggttatca agaggtgcaa
    16921 acattgtcat gcccaacctc acgccgcccc aatacagagc cctctacgaa atatacccag
    16981 ccaaagcatg tattgacgaa gcggccgagg actgccacgc ctgtatgaaa gggaggatca
    17041 ggcgaatcgg tcgtattccc ggaaccgggc ccggaggacg gaaaaaaggc ggcattgctc
    17101 cgcaataaca ccgccgtcag ttgatgatcg aagtgacttt gacgggtttt tccacaacgg
    17161 caccggtaag aagatcccgg gcacgtacca cataacggcc cggctgctgg ttcaatgaca
    17221 ggggaaaata gcttgctcca tgcccttttt caaggacagc gacatgccga taccacggta
    17281 tcacagtctg atcagcacca agaagatcca gaaggatcag tctttttacc gggtcttcct
    17341 ccgcgccgtc acttgtcaga cgtatctcca ggcgctgacc ttctgcagca gtttccgttg
    17401 aatgtatttt taagggcttc gcgtctttgg ggaggtgaac gagagccaaa ctttctccgc
    17461 cattcaggcg gatcttaagg cggtgtgggt gtgcaactct ggttcctgtt aaaggcagat
    17521 atacgctgtc ttttttcgtg aaatgaaggg gcagtggctg caccggtgcg gatggtgcgg
    17581 caagtattac atagatgacc gctgaatcat aacgaaaatg gtacaccatg ccctgaaaac
    17641 cgggatcggt cggaacctgc tgtggtgagg atgcagaact gtcagttatg aattcattaa
    17701 gacatgccat ttccgccagg aagtcaccaa caggcatgcg aaatggatgg ttgagcagca
    17761 gcgtatttcc ctgctcccag cggctgataa accagacagg cagatcaccg gctttggcaa
    17821 gggccacggc gcggtcgggt gtgatacccg catctgcctc aacctgcccc gatacacgtg
    17881 tcctcccttc aacgtcttcc gtagtgacaa gcagctctgt acactggcga ctgtccggtt
    17941 tttgatcaac gccaaaaaga gatgcaaaac ggttattcag ccgttttcct ccatgcgtat
    18001 caaaatttcc gggaagcacg tcggcagcaa ctgttccgcc ccgggcaaca aaagtaaata
    18061 tcgcctccgt ttcttcatca tccagtgcga cacaaaaagg aagaatcaga gtggaaaacg
    18121 tatcctgcct caaaccgtcc agtcgttctt tatcaataaa aacaaagtcc cggttgctcc
    18181 ggcgcaaaca ggcggacaca gcgttttgtg aatccagaaa ggtagtatta tacccggatg
    18241 cagattttgg aagatgcctg gaagggtgac tgtcatagac agcgattgca ggaggtttct
    18301 tttcagaagc aagaatcaga ggggcaatcg tatcctgtaa ctgtcgcaca gtacgggtta
    18361 tcgctgtcaa cgcttcagag agagaaccct gtacatcaaa ccaggtgttc ccgtctgctt
    18421 tttctgcttc gcctgacaag gcttcaagcc acaagacagg aatctgatgg gtcagcatct
    18481 gccagggaat ttgcagaaat gccgcaccgt tttgtccggc atcagaaggg tgcagcacca
    18541 gaccgttcca gttgcccggt gcggtataag aggcgatttt ctcgagaata agggggctgt
    18601 aatcggctat gacaaaatcc agagcccgac ataactcagg ccagcagtat ccgtgagaag
    18661 ggttggtatc tgaaaaaaaa cgtgctccag cgacagctga gccatcaatc agccgtatct
    18721 gctcatggag ccactgatga aatccggcaa actgactgtc cataaaagtg ctgaaatcca
    18781 cctggtaaga gacggtccgg gtctgttcgt tgacaggagg aacgtcgacg gtatcgatct
    18841 gctcccattc agaaaaatgc gttccccatt gccggttcaa ggcttccagg cttgtgtact
    18901 tctcccgaag cacctgatgc cacgcttcaa ggcagtatcc gcaccgacat acagcctcct
    18961 ccgtagcaca cagtacattg ccgttaccca aagaatagcg cccacgggtt cccgcccaat
    19021 ggcgcagcgt tcccgttttg agcgtttcct caagagaaat ccggtaggat gggtcattca
    19081 ggcaggggac tctgtacttt ccatccaatg cctcattaac ggagatatgc tccagttcag
    19141 gcagcagcgt tagacctttt tcggaaacac ggatgatcgt actttctccg gcgggagcat
    19201 gaacggtacg cactccttcc tgcgccaaca ttttcagcag agacagatcg ccatattcag
    19261 agggtgccgg cagggaagcg cccagttcca tttgaggagg ctccagccgc tggcgcacac
    19321 tcaggaaacg cacttcccgg taggcggaat gcagctctgt ttctgaaaag ctccggggac
    19381 cgccttcgac cgcatagacc tccagccgaa tcagcggcga aataagatcg gtaaaatgga
    19441 gccgtactgt tgcagtgccg ccgtcgcttc cgacactttc cacagactgc gcgacaaccc
    19501 gttcgaagcc atctgtagcc cgggcataaa ggcaggcctg gcgggactga ttggcaaagg
    19561 gacgcacttt ggcagaaata tccaaagagt cattgggcat gagccactgt ttctcaagtt
    19621 ccagctgata aaaatcaggc cagccgggca catgcagaag cgccgtaaac cagtcggtaa
    19681 ttccgctgcg gtctttcagc cagacatcaa ataaacaggg tcccggaccg gcaggcactt
    19741 caacaatatg aaactcggaa tctttggaaa aagcatttgc ctcttcatag gtgatgcggg
    19801 tactgctgcc ggggcgcctt atctgcaatt ccacattgta ttttttatct gccggtttgt
    19861 taaggcgaat gacaaaagga cgggagggtt gcgaaacggc tgaatctttc atggcctgaa
    19921 taaattcagg gtaaaaatcc ggcggaatct catcttcagc aggtcccgaa ggtgcaccgt
    19981 cctcgatacg cgctattctg acttcaggac gccgtcctgc ggcgtaacag atggcacgaa
    20041 cgacaaaaga ccaggcattt tcctcccagg cgctttccag gtcataagga tcggcggggg
    20101 actgaaccag agcgtttcgt cggggaaaat caccgggata atccagacag actatcttcc
    20161 ctttaccatg cacataagag cgaataatct ggcgaagctc cagcccgccc ggaaaagcac
    20221 attcgcctat accgccgctt attgaatctg tatccgcttc ctcctgaagg ttgtcaaaaa
    20281 aagcagccag tgggccatcg acgggagatc ggtcaaaagc agttagaagc ccggtacctt
    20341 gcgaaacttt aaacagtact tcggacaaaa tccgctccgg aagaagggaa atgttaaaat
    20401 ttgcgagaat caggacatca gcgggctgcc gtagctcttt ttccagccgt gaaaaaactt
    20461 ccgcatccgt ctcggcggat gaaatagttc cataggcgat gggatcgtaa ccggcatgct
    20521 ccgcatccca taaaggaacg accctgcagt ctacatccag acgtttcttt aattcagcag
    20581 cgtcctgcaa cgtatgctgc ggggcaacca gcaatacttt tacagcaccg cccgccagtg
    20641 gcttgcccca gggctgtgcc gttcctgccg gaactggaac cgcctttttg ccataggaag
    20701 ggagcgcatt tacggacggc accgctgtca ggaggcagaa aaaagccggt aaaagaaaat
    20761 aactcttttt acacaaagac atactgcctt atagtgcggc tcagaagttc ggttaagaat
    20821 gcaaaagccg gagccgcctg attaaacagc atccaaagac caaccgcctc tgtactggcg
    20881 ggtaagcaat gcattcatcg caggattatc tgtaatcact tctttttcag gatcccaatt
    20941 gaaaccgaca ttcaaagtgc aggcaatttt tccaagatgt cctattgatg cagagcggtg
    21001 tccggcataa accggagcaa tacattcttc acggctttca atacattgca ggaaatcggt
    21061 catatggttc gtgcctttcc tgaagcggaa agcagtgtca cccagacgcg tttgaagcag
    21121 ttcgggatcg ctcgccttga taccgcttct gtccacataa agccactgat cattatcacc
    21181 gaaaaacatg gtgcccagat ttccacattc tttaaataaa tcgggcattt tgtcggagct
    21241 gcctacagtc atggttgttc cgtcggcata agtgcattca aactggtact tctgaggggc
    21301 attgtataag ttgtttcttt cagggctcat ctcccaggcg actccctgca catgaaccgg
    21361 tcctgtatga tcccaattca tagccatgtg agcgatatca ccgtggtgac cgatccagtc
    21421 catcatctga cttccgccga aacccatcca ccagcgccag tcccaatgac agcgtttttc
    21481 gtgataaggc gcccattctg caggacccac ataaaactcc cagtccaatt ccgcaggagg
    21541 aggcgggaat tcatccaccc aaagcgcatt gccgccattg ggcaatgcca ccagaatgtg
    21601 cttaattttt cccagatatc cattgcgcac cagttcacag gcagttttaa aatctttacc
    21661 ggaacgctgc atgctgcctg tctgaaacac ccgcttattc tctgtaaccg ctttgacaac
    21721 ggcctgtcct tcaccaagtg accaggttag gggcttttcg caataaatat ctttcccccg
    21781 atgtgcggct tctacacaaa tgagggcatg ccaatggtca ggggtagcga caataacggc
    21841 atcaatgtct tcccgtgcca gaacttcgcg atagtctgta tagacagtac agtcttcatt
    21901 atcataataa tgatccactt ttgactttgc ctcggcaagg cgcctgcgat caacatcgca
    21961 aacggcaaca atgcgggtgt ttttgtgttt aatcgcattt tctgtgagcc cgtttgcctg
    22021 accgccggaa ccgatgactc caatggtgat tttgcttccc gggcttttat gcgcttttgc
    22081 gccaaaaact gtggacggca caatcaatgg agctgccatg gcaagaccgg cgttgtttaa
    22141 aaaagagcga cgggaaatta tctttttttc gttttttttc acagagcttt tccttattgt
    22201 taaagcggca ctgccagttt taaagacttt gaggtctgga aaaccatttg ccatggtctc
    22261 ttacaatata acagggcatt ttttacgaaa agtgcttgtt tcagtatgaa acatgattgc
    22321 agttattgtc aaatcggctt ttatccacac cttatgttat ggtataaaat ggctgtacag
    22381 tgtgcgccga atctttcaag gctgagcggc gttacagtat ttccgatagg caccggttca
    22441 ttaccttctt tttacaggtt tcgacacctg ccgagatttt cattttcagt aaccagaaca
    22501 ggaaagaagt tctcatgagt aaattgctaa gctcccatgt ttttacctct gaatctgttt
    22561 ccgagggaca tccggataag gtttgcgacc agatttctga tgcgattctg gatgcgtgcc
    22621 ttgccgcaga cgaaaacagc catgtcggct gcgaaacgct tgccgcaacc gatttcgtgg
    22681 caaatgccgg agaaatcagc tgtgaaggct ggagcagcat cgatcctgaa agtctggcac
    22741 gggctgttgt caaagacatt ggttatattt atgatgatat cggtttctct tataagacct
    22801 ttcaatatgt gaatcggctt catcagcaat ccgccgatat cgccatgggg gtcaatacaa
    22861 acgaagaaac acataaagaa cagggtgcag gcgatcaggg catgatgttc ggttatgcct
    22921 gcgacgacac gccgcagctg atgcctgctc ctctgcaata cagccacaat ctgcttattg
    22981 agcttgcccg catcagaaaa gcgggggaga ttgcttattt gcgtcctgac tccaaatctc
    23041 aggtatccat tctgtacgaa aatggaaagc ctgccaaaat cacaacggtt gtaatttccc
    23101 atcagacaga agatgttccg ctggaacaga ttcgtaaaga tatgattgat gtggcgcagc
    23161 aggttctggg tgctacggga cttctcgaca acgacacaaa gtatcatgtg aaccccaccg
    23221 gacgttttgt cataggtggt ccccatggcg actccggtct cacggggcgt aagattattg
    23281 tggacagcta cggcggagtc ggctcccatg gtggcggcgc cttttccggc aaagatccga
    23341 caaaggttga tagaagtgcc gcctattaca gccgctatgc agccaaaaat attgtcgcgg
    23401 ccggacttgc cagaaaatgt gagattcaag tggcttatgc gatcggtgtt gccgagccta
    23461 tgagcattaa cgtgtcaact ttcggcactg gcaaagtcga ggacactgtg ctccagcgtg
    23521 tactcgagaa ctccggtctc tttgatttcc ggcctgccca gattatggct gaactgagtc
    23581 tggcaaagcc gcagggctgg tgctaccgtg acacctccaa ttacggtcat ttcggacgtg
    23641 atcttttccc ctgggaaaaa acagacaagg ccgatgcctt gcgtcaggca gtcggaaaat
    23701 gacagacacc cctacggcat cccgaattct gggggttggc tcacccatca tggacatcct
    23761 tgccccggtt cccgattctt ttcttgaaaa gatttcagga gctaaaggtg gcatggaatg
    23821 gataaagcac gatgaaatgg atgcgcttct ggcgcaaatg cctgaaccgc cacagcgtgc
    23881 cctgggcggc agtgcggcaa acaccgtcgc tgctttaggg cggctgggca tgccggtggc
    23941 acttcttgga aaaatcggca gagatgcgga cggtgatgca tatcgagctg cttttacagc
    24001 ttcgggcggt gttgaagaca gcttcaagta tgatgactcc agccctacag gccgatgcct
    24061 gagcctgatc actcccgatg gagagcgcac gatgcgtact gatctgggcg cagccatgct
    24121 gatagggaag tccgatgtca gcgaccggga ttttcagcag tgcagacatg tgcacctgga
    24181 aggctacggg ctgtttaact gtgaatatgt gttccatctg ctgcaaatgg caaaagcagc
    24241 cggctgcaca gtaagcatag atcttgcctc ctttgaagtg gttcgggaca gcatggatgt
    24301 cctgccggat ctcctgaaac actatgtcga aattgtcttt gccaatgaag atgaagcagc
    24361 tgcattttct ggaaatccgg atccggaaga aggattgcgg gctttgcacg cctgttcacc
    24421 ggttgccgct gtgaaactgg gtaaagatgg cgcacttatc cggacagaag acgcctgcag
    24481 caaagtcgat gccgttcctg tagcccgggt aatagacaca accggtgccg gtgactgctg
    24541 ggcggccgga tttctttacg gatatctaaa tggctggtct ccggagatgt gcggggaatt
    24601 tgcgtctcac gtaggagcag aaactgttcg ttctctggga gcctctccgg atgcgcaagg
    24661 ctggacgcgc atccgacagg cggaagcaac tcttaaaaac agataacttt tttagcctgg
    24721 ccgcggttat gaagtccggg caacaacggg agatacatat gatgtatgta ggaagaattg
    24781 taagtgtcgg aaaaacagca gacgggcagc tctgcgccgc ctatcgtgtg tcaagccgct
    24841 cttttcccaa tcggagcgct gttgtgggta atggaaaagt cagcatcgtg cccaagccgg
    24901 ggtttgaaag tgatattttt aaaaatccct atatcgccta caactgtgtg cgcattgttt
    24961 gtgacggtgc tgttgctgtt gttaccaacg gcggacagac tgacttcatc gcggaaaaga
    25021 tggcaggagg gatgtccgca cgggatgccc tgattctgac ctctttttct atggactatg
    25081 aaaaagatga ctacaatacg ccacgcatca gcgccgttgt cgacaagcgc agtgacgagg
    25141 ggtggcttgc tgttgtgcgt catgacggac ttcaggtaga gcggatagcg ctgaaaaacg
    25201 gcttccagtg gcatcttgcc acctatgaag aaaatttcat ctgcccggaa cgttttgatg
    25261 catttccggc aacatctgcg gagagtgctt gtgcgtatat gctaagcggg ggcgtctttg
    25321 cggaaagaac aaatccggtc acagcggtcg ctgctctggc tgaagaaagc ggtttcgctc
    25381 tggctgttca ggattttcag gcataagcac agcgcccctt tggcggcatg cgttctttta
    25441 taaatcattt cttcgtaacc gtcaccatcg tcgaaaggtt gggaattcct catgagtgat
    25501 ctctatgttt ctcctctggt tgagcgtttt gcaaccgatc ctatgaaacg tttgtggagt
    25561 gcacagaaaa agttttccac ctggcggcgc tgctggattg ctctggctga agcagagcag
    25621 gaactggggc tcgacatttc agatgcgcag ctagaggaga tgcgggctca tgttgacacg
    25681 atagactttg aacaggcagc tgcttatgaa aaagaaacgc gacacgatgt aatggcacac
    25741 attcatgcat ttggcgatct ggcgccgaat gcacgtccga ttatccatct gggggctacc
    25801 agctgctttg ttggagataa taccgatctt attctgattc gggaaggtct ggaattgatt
    25861 cttgcaaaag cggcaggagt attaaatcga atcaaaacct ttgcgcttca atacaagtcc
    25921 ctgccgacgc tcggatttac ccattatcag ccggcgcaat tggtaactgt ggggaaacgt
    25981 gcatgcctct gggcacagga tctgcttctg gactgccttc agattgaaca acttctggca
    26041 tccatgcgat gccgtggcgt caaaggtgcg acaggaacac aggcttcttt cctcgctctt
    26101 tttcatgggg atgcggaaaa agtaaaggct cttgatcatc ttgttgctaa aaaactgggc
    26161 tttgaaagtt ctttcattat tgccgggcaa acgtacacca gaaaaataga tacacaggtc
    26221 cttaatgcgc tggcgggcat aggagaatcc gcccacaaat ttgctacaga tatgcgcctc
    26281 cttcagaatc tcaaggaggt ggaagagcct ttcgaacgtt cgcaggtagg cagctccgcc
    26341 atgccgtaca aacgcaatcc catgcgtagt gagcggattt gtgcgctttc acgttttctt
    26401 atgaccgcac cgctgcacag cgcattcact acggcaacac aatggtttga aagaacgctt
    26461 gacgactccg ccatacgcag actcagcctt cccgaagctt ttcttaccgc cgatgccatt
    26521 ttgaatttgt acttgaatgt catggaagca cccagggttt atcccaaagt aattgaaaaa
    26581 agaatctgga gtgaactgcc tttcatggca tcggaaaaca tcctgatggc gtgtgtacaa
    26641 aagggaggag accgtcagac gctgcacgaa gttatccggc agcatgctca ggaagcagga
    26701 cgccgggtca aagaggaagg agcggacaac gatctgctgg atcggctggc tgccgaccct
    26761 caaattaaca tgacccggga agaaatggag gagcagctga agctcgatgc atttgtcggg
    26821 cgcgctccgt cacaggtgga tgagttcttt gttcaggaag ccaatccttt tcttgaacgc
    26881 cataccgcaa gcctggatac gcattcggac gtacgtgtat aaggattcgg ggactgcatg
    26941 ccaaaaacgg ttatgccagt ttcctgtgcg cttcccgaag aagctgctct gtcatctccc
    27001 agtctatgca gggatcggta acgctgcagc catacctaag atccgatgcg ttcccgctca
    27061 gtttctggtt gccctcctga agattgctct ccagcataac accataaatg ctcttgttgc
    27121 cttcgagccg ctgttgaatc acgtcacgga gcacatgagc ctgaaggcgc gggcgctttc
    27181 cgcaattggc atggctgcaa tcaacaataa ttcgcggttc caacccggct gaccgcatct
    27241 ggtcttctac tgtaatcaca ctgacagggt cataattggg accggagcgc ccaccgcgca
    27301 aaatcacatg tccatagctg ttgccccggg tatgaaccac acaggaacgc ccggcaccgt
    27361 caacaccaag aaaatggtgg gggcggccgg cggcaatgag cgcatcgatg gcactctgca
    27421 aattgccgct tgtattattt ttaaatccga cgggcatgga cagtccgctc gccatttcac
    27481 gatgtgtctg tgattcgctg gtgcgtgctc cgatagaaac ccaagacatc agatcggcg
//
LOCUS       DAOPWB010000026        27409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1728_length_27409_cov_5.546063, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000026 DAOPWB010000000
VERSION     DAOPWB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 27409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27409
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_27409_cov_5.546063"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PKN92_04055"
     CDS             <1..836
                     /locus_tag="PKN92_04055"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ47999.1"
                     /translation="YDLIPIENGPPVPGVHMQEDIEYGRGGDTPLFLDLYTPENRQGV
                     LPGILLFFGGSWKYGRKDQLRVYAQYLAANGYVVGAVQYRLREAGQWPNSIQDAKCAV
                     RWMRAHAAEFGIDPGHIGVLGNSAGGYLSLMTAYTAGQPEFEGDGGWADEDSSVQAVV
                     DIYGPTDFTEPIRRDHNAVVSYMGGRYEEMPERFAKASPILQLSEKSPPTCVIHGTVD
                     QLVPVTQSDRLVEKLQDFGIPYEYSRINGWPHAMDAAVAVSNHTKSLALHFFNRYLKQ
                     K"
     gene            892..3288
                     /locus_tag="PKN92_04060"
     CDS             892..3288
                     /locus_tag="PKN92_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ48000.1"
                     /translation="MFSDNRSVFRLLTGSILFAGLLAFFCWYSPCSHAMSVEEEAAYI
                     VSQMTLEEKIGVLVGDGRYVPDFEKNTNDAGQGMIIEDMRSQVIIPRLPRLRTTAMSD
                     GPAGLNREAKKDGEENFEYTTAFPTGTCLAASWNMELVKRIGDAFGNEVREYGYELIL
                     APGVNLHRNPNDGRVFEYYSEDPLLSGKSAAAMVQGMQQHGIGATLKHFMAHNQQANR
                     HHFNAVISQRALRELYLRSFEIAVREGHPKSIMTSYNRMNGLYTDENVELLQLLTRQE
                     WGFDGFYITDFDGKGSALGKVRAGINLLMSGSPEEFDELLTAVKNQKLDEKLLDERLT
                     KYIEFKLSCPYALGEEISANPDLEAHAALAREAAAEGLVLLKNDNSCLPLEKAERVAL
                     FGKTAYSLIPYGTGSGEVRSHKRSLSMHDGMKELGFQLVEGMDKDYLDYVEKIKRENK
                     VPDYFNNPKMRADNGIVGDQAPKHFKGRLVAFSRERVLSREEVEGYAGRSDVAVICFG
                     RSSGENYENGYLPITDIERTLLETVCDVYHSAGKKVVVVLNVSGVWETASWSDLPDAI
                     LMAWLPGQEGGGAIADVLSGKANPSGKLPDTIPVKYDDLPSAPYYPGTPKEEPLNSLY
                     NEGIYVGYRYFNTFDVPVAYAFGHGLSYTTFEYSDLKGDKEIFDGSITFSVKVTNTGD
                     RAGKEAVQLYLTAPMDKLEKPEQELKGFAKTQLLQPGESETLQFVLDGRALASYWSGI
                     SAWVADAGVYVVSVGASSRDIRQTKTFQVPDGLVVEKTHDILFPDYLLNELSRNRKAF
                     "
     gene            3490..4059
                     /gene="raiA"
                     /locus_tag="PKN92_04065"
     CDS             3490..4059
                     /gene="raiA"
                     /locus_tag="PKN92_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNZ48001.1"
                     /translation="MNVTITGRRMEVTDALKSYVENGIKKLKIHFDKVVDINVVLDVE
                     KHRHIVDVNLYANGVRIHGRESTADMYASVDAVLTKLDRQIRKYKGRINRHKQRTGPE
                     NISYSHAILALDAMDAETDVEPVEEEHHRIINREKRPLQPMSIDEAIMQLNLLEDSFF
                     VFLNADTAQVNVVYACEDNTYGLIEPEVG"
     gene            4111..5082
                     /gene="hprK"
                     /locus_tag="PKN92_04070"
     CDS             4111..5082
                     /gene="hprK"
                     /locus_tag="PKN92_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNZ48002.1"
                     /translation="MDFARLPVKRKRFVPVSRLLNREDHGMDFELLAGFQGIDRPVFT
                     WDVNRPGLALSGYLDYFANDRVQILGNTEIHYMERVEPAELANRIANMFAFEIPAFIL
                     SRNLMPQPIFLDMCNRYGIPVLRSTMSTDEAISRIILFLAQEFAPESNLHGTAVDVYG
                     VGCLIVGKPGIGKSESALELVERGHRLVADDYVEIQRRENSIHAKTNHVLKHFIEIRG
                     LGMIDLRSVFGVGSVRDFKRIGLVLELAEWSTVTEFDRLGLTTSFTEILGVRIPYLRI
                     PVRPGRNIAIIIEVAALNWRLKELGIESASLLDEYARANNRRGGRRF"
     gene            5120..6220
                     /gene="rsgA"
                     /locus_tag="PKN92_04075"
     CDS             5120..6220
                     /gene="rsgA"
                     /locus_tag="PKN92_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNZ48003.1"
                     /translation="MARRKGLGRKKRSTVREKMWEEKHEDSFTHDLARHRRGRLRLSE
                     AVDAVPGQPEGKCLPNATVITHSRRWAFVSYDDGLSDAPENPGPLCRIDERLREEEES
                     LLAPGDRVQVQFEKGEGVVVAAAPRKTRLMRLGGVHSRIKQQVLAANIDELVIVASVK
                     KPLFSPGLVDRFLIAAQQGGVEPVLCFNKIDLDAPPAAFLGLYQELGIPVFCTSCITG
                     EGIQELEDCLAGKLCVFAGHSGVGKSSLLNAMDPELKLVTREVSEATLKGRHATTAGV
                     LYMLHRDIRVIDTPGIRALGLWGLSAEEVSLYFPEIATESLYCKFRNCTHIHEKNCAV
                     QKAVQQGVIARARYESYRRIRSGIEAMKPDWL"
     gene            complement(6251..6604)
                     /locus_tag="PKN92_04080"
     CDS             complement(6251..6604)
                     /locus_tag="PKN92_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48004.1"
                     /translation="MQVSSEECMPSSLTRREVRAIQKFYEQVFNTETRPSTFEEMARH
                     WQRHYAAKWHVFDHVQAMAMQRDEIARHKWILSEKAGYDLGDWAAHNWVFLYASLWRE
                     WYETACDIYGLDLLV"
     gene            complement(6749..7645)
                     /locus_tag="PKN92_04085"
     CDS             complement(6749..7645)
                     /locus_tag="PKN92_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ48005.1"
                     /translation="MRVKSLWVLLPCIAMAIMVSMSFTALADTPGPGAPAAEALGWRI
                     GCQLWSFNKFSFTEAIDKTASLGLKYAEAFPGQRFCADLPEDIKFEHTMSDEHRAQAK
                     EKLAKAGITLLSYGVVSLPSNEEKARQVFEFAKDMGIKTIASEPLKKNLPLVAKLADE
                     YDIKVAIHNHPQPSLYANYETVLEAVNGLSERIGACADTGHWPRSGINALEAVKALQG
                     RLISFHLKDINEFGVKDAHDVIWGTGVCDVEGILTEIARQGIKEPQFFIEYEYNWDNN
                     LPDIAACVAYFNQIAENLGAAK"
     gene            8225..9148
                     /locus_tag="PKN92_04090"
     CDS             8225..9148
                     /locus_tag="PKN92_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ48006.1"
                     /translation="MSDITICIVEDYEDEALSLSHQMALVGYKTVIAGSGEEALRLCR
                     EGNIHLVLLDVGLPDMDGYEVCRRLKADPATVDIPVIFVTARGDAVDVELGHALGAVD
                     YVPKPYNLPMIVVHVDAALRTRQSQLDLVSGPFMLVDPAYTDQLTGLRNRRFLMERLQ
                     EESEKSQRYHYPVSCLILEVEDGVQEEPEEDLSDELLVEIAMTLRKNSRAFDIIARYD
                     ETLFAAVLPHARMDDAVAYGKKIKQELIQMASRSSCVLKDPRISMGITACLNGERPGS
                     AEEFLGSSMNKLLLAKSNAPEHIVAGEYSGG"
     gene            9166..10056
                     /locus_tag="PKN92_04095"
     CDS             9166..10056
                     /locus_tag="PKN92_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNZ48007.1"
                     /translation="MELSFLPEEGVLNRETSSVWHIGTAGWSYPDWKGIVYPPSGVRG
                     NQNLAALSRLVDAVEINVTFYRPVSPGMVHSWLKAVVPRPAFKFTAKAPSLLTHTRSA
                     VPDKKVACTFSESMRPLRESGKLGALLFQFPWSFKRTRENRSYLSQLLDLFEELPLCV
                     EVRHSSWDHPDFFRGLSERKVAFCNIDQPLLDACIAPSARVTADFGYVRLHGRNTAQW
                     FDGTAGRDGRYNYLYTPEELAEWVERILVLGKKVNDLYVITNNHYRGQSVVNALEIQQ
                     ALDLPARPAPETLRFLYMRP"
     gene            10158..13013
                     /locus_tag="PKN92_04100"
     CDS             10158..13013
                     /locus_tag="PKN92_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ48008.1"
                     /translation="MTILEKLQQNCRCPIFLDDFTRQLYATDASIYQCVPRGVAFPRS
                     AAETAELMRAAAAENIPLIPRGAGSGLAGGAVGDGLVIDLSRYNHFISDFNQDAQTVR
                     VGAGVVLDQLNEHVAPYGLTFGADVATSSRATLGGMIANNSSGARAPLYGVTMDHVES
                     LEVVTADGRVHTLSASSGDMREKLARLQGRVLQERAEIEARFHREICKRWPGYGLDRY
                     LRSILADAADPAYLIGGSEGTLCTVFSATLKLVPLPPGTGLILLFFDTVQEAMEATAA
                     LLDLKPASVEHIDRLLFDQTRGNRAFEQARALLELDEKPCEGILLVEFYDHIEEKLQQ
                     VQQRKLGRRSLLCKTEKEKALVWHLRKAGLSLLTGCAGDKKPVAGIEDVAVPPAQLPR
                     YVEALRSVIDRLGLKASFYGHAAAGLLHVRPVLNLRRAEDIRAFRTLAEEVSALTLAF
                     KGSLTAEHGVGINRTEFVKEHIGAPLLKIMEEVKEAFDPGGLMNPGKIIDTGRWRLDA
                     DLRMGADYALPVPFEPVLLFRAKDHSFSGNLEQCNGCGGCRKAPPAMCPTFQATGEEY
                     LSTRGRANIIRAVLEGRLDPGNAPLLSESLEAALSSCLSCKACTTECPSNVNMSLLKA
                     ELMHARQRRHGVPFHARLLSRVDLLGKIGSAFPGTANAFLSFAPFRQLLRKVAHIAPE
                     RPLPLYAPERFDRWFKKRTAQKPKGYRGTILLWDDCFVRHNEPHIGHAAVRILEAAGF
                     VPKLIEGHACCGRPAFSMGRLDLARKFGRKNLDLLKKTDGPILFLEPSCYAMFREEYG
                     ELGLDGALQVAERAVLFEQFIEQLLEKEPEAIPLKALPFGLAIHAHCHAKMLADTQIH
                     ARLAQRLTEQNAAMLNSGCCGMAGSFGVLEDKYALSLAVADPLLAMIDALPGDTKVIA
                     SGASCRHQLAELSARPPLHFAEVLEQALRTSSEKELR"
     gene            complement(13006..15108)
                     /gene="feoB"
                     /locus_tag="PKN92_04105"
     CDS             complement(13006..15108)
                     /gene="feoB"
                     /locus_tag="PKN92_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNZ48009.1"
                     /translation="MSPGKIVIAGNPNAGKTCLFNALTGSAQQVSNYPGVTVEFKSSV
                     MRWQGHRVTVVDLPGTYSLTAYSQEELVARNYILDEHPAVIVNVVDASNLERNLYLSI
                     QLMEIGIPMVIALNMVDTAERRGYKIDAETLSRLLGVPVIPTIACRGKGIDEIAAASM
                     AILLDKEQARPAPLSYGHGVDDEVLALTEEIRKIPALNTHLSPAWLAVKVLEKDERAV
                     EEIKKRAPEETAELFLKIEDAVRRIERHFNADASTLVAEHRYGIASGLVKRCVTFNAK
                     ARRDITEQLDLIICNRIIGPIILLLVVAALFFWVFKVSDEWKWIPWFGGFQSPTHFME
                     SLFLSLAALIAPLKNSMPMLHSLLNDGIIGGVGGVMSFVPLIFFMFLFVAALEDSGYI
                     ARVAFILDRPLRIFGLQGKSILAFIVAGGLGAGGCAVPGIMSTRTMREDRDRLVTMLV
                     TPFMNCGAKMPVYAMLIAAFFPNNRTLVMMILWALSWLTALAAAWILRRTLVRGEQMP
                     FVMELPPYHMPVLRGVLRHTWERTWMYMKKAGTVILAINLVLWALMYFPHPAEETNPE
                     EKLAYSYAGRAGKALEPISRAAGFDWRTNIALIGGFAAKEVVLGVLGTAYSLENTDEE
                     NYLSLSEQLAKRSDWNPVRAFALMVFVMLYAPCLVTIAVIRRESGSWKWALFSTVYST
                     AFAFILAIAFYRIGSLLI"
     gene            complement(15175..15447)
                     /locus_tag="PKN92_04110"
     CDS             complement(15175..15447)
                     /locus_tag="PKN92_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNZ48010.1"
                     /translation="MFDLKTGDTAKILGIGTDTPLSLRRRLLDMGITKGAELRVERHA
                     PLGDPVEIFVKGYFLALRMSEARTIEVEQIKSSSESLLAASESSLD"
     gene            complement(15456..15677)
                     /locus_tag="PKN92_04115"
     CDS             complement(15456..15677)
                     /locus_tag="PKN92_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48011.1"
                     /translation="MELLIVLFIVLLAFTLLVRHGIRSMRRDQSPCCGCSTCSLPEEL
                     KEQCASAQKADPKSALPLGTSGMVQGDRS"
     gene            16068..18338
                     /locus_tag="PKN92_04120"
     CDS             16068..18338
                     /locus_tag="PKN92_04120"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNZ48012.1"
                     /translation="MARISSFAVLLRIALFAVFLSCAFIAGSYLGKKSVPEMPSLTAL
                     PAHQDTLPEGGEQSGEHALPLLHSSAQAPQLFFFAGKASDDVWPVVAEEVTLAKEAGI
                     SNYVVPVALDWSEPYQPEKASAIEKQLQQYLDINSRAQLLLWINLNPPASWLTLYPDA
                     ALRFQSGLQAYPSIGSELWRNRALEILAGEIDKLEKSGLADHIRGYVLGALGEQRWRL
                     PGEHDLSEANQSAFRRWLQNHYGTEEELAKAWGVDAQTFDRCAVPVFSQQEGQEKQAP
                     VFLVLPQMQPMMDYYRYCSDQVADTLISAVTRIKEIARDNVLVLAPYGFTYELTEPSS
                     GHYALERLLHSDLDGVILPVSYVDRGLGGTGGPVGPVDSLLARGKTCFIMDDTRTGVE
                     RDESTGLFGRVKGIRPDDIFEVQKRNFALALSCGMGLIWTDPESQGWLNIPDQWKQFA
                     RLKTLYADYQNKELQGQTPESPATLTVVVDERANFCLASGDRINNALLLKGREMALRA
                     GVSTRFHLLKDVVDGVAPPSPVYLFLNPFMITEEERNLLHARLAGEEACAIWLYAPGY
                     FGAESGMEGMSRLVGMDIQPFKEPASGESVYILSGLYMPPDQPFGSKTLWNPLFFIPP
                     HDDLDLLAHYTSDKDKGSIAIRTFPENWTSLFIATPELSPALLRELMVLLEQPILTND
                     AEDIWYDTVYARNNLMTIHSNQAGRRSFSFGQYYDIEDLFDSSMSWFQKDTILLTMRT
                     GETRVLQKKIIETEKR"
     gene            18432..21395
                     /locus_tag="PKN92_04125"
     CDS             18432..21395
                     /locus_tag="PKN92_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNZ48013.1"
                     /translation="MLNVDRRSALCRFIFYGWLFFLVLALIPWTANPVKPIKDLVTAA
                     AALAVCAAGIGLLPRTVGDMRKKLSPPLCILGLWLCWSVVSALASRHHWLAVDELRIL
                     IPWLVLSFYASLLFHSGQSRKTLALVICLAVCLSSLYGLCQYLGFDPFPWVTRDIEEY
                     RGLPATFGNPNFAGHSLILVIIVAAGLIAAAPRSAAWLLLPLPLFHLYQTHMRGALLA
                     LGAATLLVLLYVFFRNRPGSSLKKISCLFAVLLLIALAGLAFLLPLSKKMSGYYFPTG
                     GSLILRYNGYYGAARMALDHPILGVGPGNYSPENIPYWTFYERRWFADQNKRNTHVHC
                     DLLESAVETGFPGMILHLLLFILLIVQSLYVAFESGELEKRRWGVIFAAFFLAFLVDG
                     LFGFNLRIPVSGALFYLAVGVFSAFWHESTENNQGRQERPSPSAVPDRGRLPELPPQK
                     RLFMGLISIGLVSVSLGCAWWALRVFQGELDWYRGNSALAWAERNSGDAQVRPVLLKA
                     ESAAARGFKKCFWDTRYSNLLGHIALKKGDTDQAIVYYTHSHEMDPYHPHTAANLARA
                     WLSRSMQTMGRESSLADLEKNAQGLETAEKHARYAAVYCPESPYVQDVLWRIVRAEAQ
                     LAEMRGEDAVPLWKETRDLINVHLRDVPLDLPAARRALFSACIKLDDLDTVESVLTPL
                     AEDQSLSSEFWEFYRRFAREKGQMDHYLDALLKQCARALSEKSDRFVLLFGYLSAAWA
                     EVYTAEGGLDFYIDEALRHFPESLALWALAIRAVPPEEAGAVLKEKTNRTGSSSCPEF
                     IKLFAAEPQDEKSVTQSASLLAREAMQGGDSSVIGDLAVFADVLLRRTESLALESGAK
                     AGIYANLATVYLEGALWEEADRYFAQAIALVPPASSARLKASRSRALEQLGRLPEALQ
                     LSREAWQQMPDDPLVLLIHGRILRLTKQNAEAAALLEKAAGRFPAGSPLQRQAHYERA
                     LAEQHTAAPAAGKGE"
     gene            21397..22209
                     /locus_tag="PKN92_04130"
     CDS             21397..22209
                     /locus_tag="PKN92_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48014.1"
                     /translation="MNKTRIREKEDSAPVSDTEKIKVPASLFSFTGVLCIISMIGILG
                     MLLYFSESGEAFLSARDDSVGEVLMKRGDRLVRAGSDKEALAYYEKALAARFAGAQNK
                     THTFEAAGLILWKQGHFDRAKDYLSRSLQGYQPTEEPYEALIDILLKEGHAEEAASLL
                     EDWRSVRDEAADPWQSDKVLLASGKAAAALGKEEEALRLFQQGAEQGGAPEFSACLAL
                     YYAKAGDREKAISCLKRYFLRGGQGEKFRALYDALLSDKPNPDSELLSLFTH"
     gene            complement(22228..23826)
                     /gene="cimA"
                     /locus_tag="PKN92_04135"
     CDS             complement(22228..23826)
                     /gene="cimA"
                     /locus_tag="PKN92_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNZ48015.1"
                     /translation="MKKIFIYDTTLRDGAQGPGIKFSSEDQLLVVKTLDALGIDFIEG
                     GQPGSNPKAAMLFEQVKDLDLKHSVMAAFGSTRHIKYSCDEDPNLKALIQTGCPVVTI
                     FAKSIVSQVKDVLRISLEDNLKLVSESVAWLKANGRRVFLDAEHFFDGYLEDPEYALT
                     VLQCAAEAGAESVVLCDTNGGQLPDAIAEMTRAAVARIPGTQVGIHPHNDSGTAVASV
                     FASVKAGAEQVQGTMNGYGERCGNVNLCTVIPGLQLKMGYDVISAEQLAQLTRRSHFI
                     AELANMALRDHAPYVGKNAFTHKGGMHADAVKKNKTSYEHVKPELVGNFTRIAVSELA
                     GRSSLIQKAEELGIHLEKDSPHTSEILRQIKELENKGYEFEGADASLLLIIRRVLNQT
                     PFLFKTLGYHARVNQPRTDGPTLSEVTVKLELPGGEIVHTVAEGDGPVDAMNNALRKA
                     LKSHYPAIEEMHLEDYKVRILDGKLATQAKTRVLIETSDPGDSWSTVGVSENIITASY
                     VALLDSFEYFLLRRYRNGQQPADC"
     gene            complement(23953..25176)
                     /locus_tag="PKN92_04140"
     CDS             complement(23953..25176)
                     /locus_tag="PKN92_04140"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HNZ48016.1"
                     /translation="MKYIILLGDGMADHPLDACQGRTPIEAAHTPNMDRVARMGVTGL
                     FCPIPEGMPPGSDVGNLSLFGYDPRVSFSGRAAIEAASQGITLAADEVAFRCNLVTLS
                     DGVMKDFTAGHITTEEASELINHLDKELSASFPIRLHCGVSYRHTGILKADSGCTVED
                     LAQAVCEPPHNITGQSFEPFLPQGAAADLLIALMQASQKALQEHPVNVARIASGKLPA
                     VSLWPWGQGRALTLESYRDRFGVSGAVISAVDLVKGIGVCAGLEVLHVEGATGWIDTN
                     YAGKVAAALNALEEHDFVYLHVEAPDEAAHQGSIPLKIQAIEDFDAKVVAPVIAYAAD
                     RKDCRILVAPDHFTLISTKTHASGAVPFALCGPGIQPGESAAYGEGAALSSGIMIPDG
                     HRLTVDFLKEETLSF"
     gene            complement(25778..25858)
                     /locus_tag="PKN92_04145"
                     /pseudo
     CDS             complement(25778..25858)
                     /locus_tag="PKN92_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            complement(26085..27152)
                     /gene="nadA"
                     /locus_tag="PKN92_04150"
     CDS             complement(26085..27152)
                     /gene="nadA"
                     /locus_tag="PKN92_04150"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNZ48017.1"
                     /translation="MNTLSAQAGLDETRKFMKERLQGIVPDIEIEAKVEIAHEINRLK
                     HEMNAVILGHNYMEPALYHSIPDYTGDSLQLSSIAAKTTADIIVFCGVWFMGETAKIL
                     NPQKTVLVPSEKAGCSLAEGISADDIRALKAKFPGAVVVTYVNTYATSKAESDYCCTS
                     GNADKVLSKLVEDGHKRIIFLPDAYLAENTATQLGLPFLDSEADQKAFDAVPADQPLV
                     IGWHAKCEVHELFTTEDVANIRKQYQDAVILAHPECPPEVIQLVDKSGSTKAMVDYVR
                     DKDAPWYALLTECSMGDNLAAEFPHRRLVRACTLRCKHMNYITLEDTLAALKQCQYQV
                     HLNETIRQQALLPIQRMLAIQ"
BASE COUNT         6514 a         6721 c         7478 g         6696 t
ORIGIN      
        1 tttatgatct gatccccatt gagaatggtc cgcctgtgcc cggcgttcat atgcaggaag
       61 atattgaata cggcagaggc ggtgatacgc cgctgtttct ggatctctat acaccggaaa
      121 accggcaggg cgtacttccg ggtattctcc tgtttttcgg cggcagctgg aaatacggac
      181 ggaaggatca gctgcgtgtg tacgcccagt atttagctgc caacgggtat gtggtcggtg
      241 ccgtacagta tcggcttcgc gaagccgggc aatggcccaa ttccattcag gatgcgaaat
      301 gtgccgtgcg ctggatgcgt gcccatgctg ctgaattcgg cattgacccc gggcatatcg
      361 gtgttctggg gaattcagcg ggcgggtatc tttcgctgat gacagcatac accgccggac
      421 agcctgagtt tgaaggcgat ggcggctggg cggatgaaga tagctcagtt caggctgtgg
      481 tggatattta cgggcccacg gatttcacgg agccgatccg gcgcgatcac aatgccgtgg
      541 tgtcttatat gggcggacgc tatgaagaaa tgccggaacg tttcgcaaaa gcctcgccta
      601 ttttgcagct atccgaaaag tcaccgccta cctgcgtgat tcatggaacg gtggatcagc
      661 tggtgcccgt gacccagagt gaccgtctcg tggaaaaatt acaggacttc gggatcccct
      721 atgagtacag ccggataaac ggctggcccc atgccatgga tgcagccgtg gcggtgagca
      781 atcataccaa atcgcttgca ttgcattttt tcaaccgtta cctgaagcaa aagtgaagac
      841 cactgtccgt cgatagtgcg cttcaatccg tagtttcgga aaggaataac catgttttct
      901 gacaatcgaa gtgtattcag gctgctgaca ggcagcattc tttttgcagg acttctggct
      961 ttcttctgct ggtattctcc ttgcagccat gccatgagcg tagaggaaga agcagcatat
     1021 atcgtgtctc agatgacact ggaagaaaaa atcggtgtcc tcgtcggaga cggtcgctat
     1081 gtgccggact ttgaaaaaaa taccaatgac gccgggcagg gcatgattat tgaagacatg
     1141 cgttcgcagg tcattatccc ccggctgccg agactgcgga caaccgccat gtcggacggt
     1201 ccggcaggat tgaacaggga agcgaagaaa gatggcgaag aaaacttcga atataccact
     1261 gcctttccga caggtacctg tcttgccgcc agttggaata tggagctggt gaagcggatt
     1321 ggcgatgcct tcggaaacga agtgcgggaa tacggatatg aactgatttt ggcgccgggt
     1381 gtgaatctgc atcgcaatcc taacgatggc agggttttcg aatattattc ggaagatccg
     1441 ctcctgtccg gtaaatcggc ggcagccatg gtgcagggga tgcagcagca tgggatcggt
     1501 gccacgctga agcattttat ggcacataat cagcaggcga ataggcatca tttcaatgcc
     1561 gttatcagcc agcgggcttt gcgggagctg tacctgcgct cctttgaaat cgctgtccgt
     1621 gaggggcatc ccaaaagcat catgacttcc tacaacagga tgaacggtct ttacacggat
     1681 gaaaatgtgg agctgctgca gctgctgacc cggcaggaat ggggatttga cggtttttat
     1741 attacagact ttgacggaaa gggaagtgct ctcgggaaag tccgtgcagg tataaatcta
     1801 ctgatgagcg gcagtcccga agaattcgac gagctgctga ctgctgtaaa aaatcagaaa
     1861 ctggatgaaa aacttctgga tgaacgcctg accaaatata ttgaattcaa attgagctgc
     1921 ccctatgcgc tgggcgagga aatcagtgca aatccggatc tggaagctca tgcggcactt
     1981 gcaagagaag ccgctgcgga agggctcgtc ctgcttaaaa acgataacag ctgccttcct
     2041 ctggaaaagg cggaaagggt tgctttattc ggaaaaacag catatagcct cataccctat
     2101 ggtacaggaa gcggagaagt gcgaagccat aaaagaagcc tgtccatgca tgacggcatg
     2161 aaggaactcg gtttccaact ggtggagggc atggacaagg actatctcga ttatgttgaa
     2221 aaaattaaaa gagagaacaa ggttcccgat tacttcaata atcccaaaat gcgcgcggat
     2281 aacggcattg tcggagatca ggcacccaaa cattttaaag gaagactggt tgccttcagc
     2341 cgggagcggg tactgagccg tgaggaagtg gaaggctatg cgggccgctc cgatgtggca
     2401 gttatctgtt tcgggcgcag ttccggcgaa aactatgaga atggctacct gcctattacg
     2461 gatatagagc ggacactcct ggaaaccgta tgtgatgtgt accacagtgc agggaaaaaa
     2521 gtggttgttg tgctgaatgt gagcggtgtt tgggaaactg catcctggag cgatctgcct
     2581 gatgccatac tgatggcgtg gctgccggga caggaagggg gcggtgccat tgccgacgtg
     2641 ctcagcggaa aggcgaaccc ttccggaaag ctgcccgaca ccatcccggt aaaatatgac
     2701 gacctgccgt ctgctcccta ttatccggga acgcccaaag aagagcccct caattccttg
     2761 tataacgagg gtatttatgt gggctatcgt tacttcaata cctttgatgt gcctgttgcc
     2821 tatgccttcg gtcacggact ttcttatacc acctttgaat attccgatct gaaaggagac
     2881 aaagaaatct ttgacggatc catcaccttt tcagtaaagg tgactaatac gggcgacagg
     2941 gcaggcaaag aagcagtaca gctgtatctg acagcgccga tggacaaact ggaaaaaccg
     3001 gagcaggaat tgaagggttt tgccaaaacg cagctgcttc agcccggaga aagcgagacg
     3061 ctgcagttcg ttctggacgg ccgtgcgctg gcatcttact ggagcggtat cagtgcctgg
     3121 gtcgccgatg ccggtgtata tgtggtctct gtcggggctt catcaaggga tatccgtcag
     3181 acaaaaacct ttcaggttcc cgacgggctg gtcgttgaaa aaacgcacga tattcttttt
     3241 cctgattacc tgttaaatga attgtcgcgg aacaggaaag ccttctgaca ggaaagatcc
     3301 ctcctttttg aacagaagcc tgcatgataa tccagcggga atcatcggtt tttgccattg
     3361 gttttatggc attatcatgc aggctgtttt cataaaacgc tgttcgggcg ttttgaataa
     3421 acagaagatg cgtggtatgc ttttatggta gtgtaagcat tggaaagtaa caacatttgg
     3481 agtttcatca tgaatgtcac cattactggc cgccgaatgg aagtaaccga tgcgctcaaa
     3541 tcttacgtgg aaaacggcat caaaaaactg aagatccact ttgataaagt ggtggatatc
     3601 aatgtcgttc ttgatgtcga aaaacatcgg catattgtgg atgtgaactt gtatgcaaac
     3661 ggtgtgcgca ttcacggtcg ggaatctact gccgatatgt atgcttctgt tgatgcggta
     3721 ctgacaaagc tcgataggca aatccgtaaa tacaaaggaa gaattaaccg gcacaagcag
     3781 cggactggcc cggaaaatat cagttacagc catgccattc ttgcgctgga tgccatggat
     3841 gcggaaacag atgttgagcc ggtggaagaa gaacaccacc ggattatcaa cagagaaaaa
     3901 aggccactcc agcccatgtc catcgatgag gcaattatgc agctgaatct gctggaagat
     3961 tcctttttcg ttttcctcaa tgcagatacg gcacaagtga atgttgtgta tgcctgtgaa
     4021 gacaacacct atggattgat tgagccggaa gtcggatgac tgaaaaacca tgacagcaca
     4081 caggcaaaaa agtaaagggt tcataccgtg atggattttg cccgtctgcc tgtgaagcgc
     4141 aagcgttttg tgcctgtttc cagacttctc aaccgggaag atcatggcat ggacttcgag
     4201 ttgcttgccg gatttcaggg cattgatcga ccagtcttca cctgggacgt aaaccgaccg
     4261 ggactggcgc tgagcggtta tctggattat tttgccaatg accgtgtgca gattttaggc
     4321 aatacagaaa tccactacat ggagcgcgtg gaacctgcag agctggcgaa ccgcatcgca
     4381 aacatgtttg cttttgagat cccggccttt attctttcaa gaaatctcat gccgcagccc
     4441 atatttctcg acatgtgcaa ccgatacggc attcccgtac tgcgatcgac catgagtacc
     4501 gatgaggcga tcagccgcat cattcttttc ctggcacagg agttcgcccc cgaaagtaac
     4561 ctccatggga cagcggtgga tgtctacggt gtcggctgtc ttattgtcgg taaacccggt
     4621 atcggcaaaa gtgaatctgc cctcgaactg gtggagcggg gacatcgtct ggtggcggat
     4681 gattatgtgg agattcagcg tagagagaac tccattcatg cgaaaaccaa tcatgtgctc
     4741 aagcatttta ttgagattcg cgggctgggc atgatagatc ttcgctctgt tttcggtgtc
     4801 ggcagtgttc gcgattttaa acggatcggg ctggtgctgg aactggcgga atggagcacc
     4861 gtaacagaat ttgaccggct tggactgaca accagtttta ctgaaattct gggagtgcgg
     4921 attccctatc ttcgtattcc ggtccgaccc gggcggaata tcgccatcat cattgaagtg
     4981 gcagcactta actggcggtt gaaggaactc ggcatcgagt cggcaagcct cctcgatgaa
     5041 tatgcacgcg ccaataacag gcggggcgga cgccgtttct gatttttgat aacagacagg
     5101 taaaagaaaa gcatactgca tggcacgacg taaaggactg ggcagaaaaa agcggagtac
     5161 tgtccgggag aaaatgtggg aggaaaagca tgaggattcc ttcacccatg atcttgcccg
     5221 gcaccggcgg ggtcggctca ggctgagcga agccgttgac gctgtgcccg ggcagccgga
     5281 gggcaaatgt ctgcccaatg ccacagtgat tacccattcc cgacgttggg catttgtaag
     5341 ctatgatgat ggtctctccg atgcgccaga aaaccccggc cctctctgcc gcattgatga
     5401 acggcttcgt gaagaagagg aaagcctgct ggcaccgggt gatcgggtac aagtgcagtt
     5461 tgaaaaaggg gaaggggtgg ttgtcgcggc agcaccacga aaaacaagac ttatgcgact
     5521 gggcggtgtt cactccagaa ttaaacagca ggttcttgcc gccaatattg acgaactggt
     5581 tatcgttgct tccgtgaaaa aacctctttt cagtcctgga ctggtagacc ggtttctgat
     5641 tgccgctcag cagggcggcg tagaaccggt gctatgtttc aataaaattg acctggatgc
     5701 gccccctgca gcctttctcg ggttgtatca ggaactgggc attcctgtct tttgtaccag
     5761 ctgtataaca ggcgaaggta ttcaggaact ggaagactgc ctggccggaa aactctgtgt
     5821 ttttgcagga catagtggcg tgggaaaatc gtctctgctg aatgccatgg atccggaact
     5881 gaaacttgtt acgcgcgaag tcagtgaagc gactttaaag ggcaggcatg cgacaacggc
     5941 aggcgtgttg tatatgctgc accgggatat tcgggtgatt gatacgcccg gaattcgtgc
     6001 gctgggactc tggggattgt ccgctgagga agtttcttta tatttcccgg aaatagcgac
     6061 ggagagcctg tattgtaaat tcaggaattg cactcatatt catgagaaaa actgcgctgt
     6121 gcagaaagca gtgcaacagg gcgtaatcgc ccgagcacgt tacgaatcct atcggcgtat
     6181 tcgcagcggc attgaggcga tgaagcctga ctggctgtaa gagggacaga ggaggaggca
     6241 gaaagctgtt ttataccaga agatccagcc cgtagatgtc acaggctgtt tcgtaccatt
     6301 cccgccaaag ggaggcatac aggaaaaccc agttgtgggc agcccaatcg ccaagatcat
     6361 aaccggcttt ttcggatagt atccatttat ggcgtgcaat ttcatcgcgc tgcattgcca
     6421 tggcctgaac atgatcgaag acgtgccatt tggcagcata atgccgctgc cagtgccgtg
     6481 ccatttcttc aaaagtgctg ggacgagttt cggtgttaaa gacttgctcg taaaatttct
     6541 gaatggctcg tacttcacgc ctggtgaggc tgctgggcat acattcttcg gaactaactt
     6601 gcatggatcc cgaccctttt gtagtcattt tcctttttca catacaaatt ataacgctgc
     6661 ttttttcaaa agtcaagata aatgtaaaaa aaatgccccg aagcgatggg tataacgctc
     6721 cggggcgggt aaacagggtt aaacggactt attttgctgc gcccagattt tcagcgattt
     6781 gattgaaata ggcaacgcat gctgcgatgt caggcaggtt attgtcccaa ttgtactcat
     6841 attcaataaa aaactgaggc tctttgatgc cttgtctggc gatttcagtc agaattcctt
     6901 ccacgtcaca cacgcccgtg ccccaaataa catcgtgggc atcttttacg ccaaattcat
     6961 taatatcttt cagatggaaa gaaatcagcc tgccttgcaa cgctttgact gcctccaacg
     7021 cattgatgcc ggatcggggc cagtgaccgg tgtcagcgca ggcgccaatt cgttccgaga
     7081 gtccatttac agcttccaga acagtctcgt agttggcata cagcgagggc tgcgggtgat
     7141 tgtggattgc caccttgata tcatattcgt cggcaagttt ggcaacgagg ggcaggtttt
     7201 tcttcagggg ttcggaagca atcgttttga tgcccatgtc ttttgcaaat tcgaaaacct
     7261 gtcgcgcttt ctcttcgttg gacggaagag aaaccacgcc gtaggacagg agtgtaatgc
     7321 cggcttttgc cagtttttct ttcgcctggg cacgatgctc atcactcatg gtgtgttcaa
     7381 atttaatgtc ttcaggcaga tcagcacaaa aacgctgacc ggggaacgct tccgcatact
     7441 tgagtcccag agacgctgtc ttatcgatgg cctcagtaaa actgaattta ttgaagctcc
     7501 ataattgaca gccgatgcgc cagcctaaag cttcagccgc gggcgcacca ggcccgggtg
     7561 tatccgccag ggcggtgaaa gacatactga ccataattgc cattgcaata cagggaagca
     7621 gtacccaaag agatttgaca cgcattattc tttccttttt tttgagttgc ggactgcaaa
     7681 cacaagtaaa cgctgttcta caggctattg tctgctctgc aggtaacctg acactatcgc
     7741 ttaatcatag cattacaccg ctgaagcatc aaaatcacct gaaccttcat cgacggaaaa
     7801 gaagactttg aagcgctgcg tctggaagca attgaatttt gaagtttttt tcggcgactt
     7861 tcttcgtttt tttaaaaagt ttgcacatat tttctgaaag tgcggtataa tcttcataaa
     7921 gtaaaaggat tataaaaaaa aagaaacctg ttaaaaagtg ctattgaaga aggttgttta
     7981 aaggaaacag gatcaagcca gtgcctgtat tttctgaaat gccattcata ttccgaccac
     8041 ggatcaagtc ggtttttctc aaataaggat aaataaaatg acccgggcgc agtgtattca
     8101 ctttccggga aattgacaga agaaacagta atgcaatact cggcagccgc ggtaaagcta
     8161 acccggcaaa actgctgctt cgggcttttc atcccgatgc atgcggattt caggagagta
     8221 agttgtgagc gacataacga tttgtattgt ggaagactat gaggacgagg cactctcgtt
     8281 gagtcatcag atggctttag ttggatacaa aacagtcata gccggatctg gtgaagaagc
     8341 gttgcggttg tgccgtgaag gaaatatcca tcttgtgctg cttgatgtgg gattgccgga
     8401 catggatggg tacgaagtgt gccgccggtt aaaagctgat cccgctactg tggatattcc
     8461 cgtcattttt gttacggcgc gcggggacgc tgtcgacgtg gagctggggc atgctctcgg
     8521 cgctgtggat tatgttccga agccctataa tctgcccatg attgtggtgc atgtggatgc
     8581 cgcacttcgt acacggcaga gccagctgga tcttgtgtcc ggtcctttta tgctggtgga
     8641 ccctgcttac acggatcagt tgaccggatt gcgaaacagg cgttttctta tggagcgtct
     8701 tcaggaagaa tcggaaaaat cacaacgata ccattatccc gtgtcctgcc tcatacttga
     8761 agtggaagac ggcgttcagg aagagcctga agaagacctt tctgatgagc tgcttgtcga
     8821 aattgccatg accttgcgga aaaactcacg ggcttttgat attatagccc gctatgacga
     8881 aacgctgttt gctgccgtcc tgcctcatgc gcggatggac gacgctgtcg cttacgggaa
     8941 aaaaatcaag caggagttga ttcaaatggc ttcgcgcagt tcctgcgtcc tcaaagatcc
     9001 tcgtatctcc atgggcatta ccgcatgttt gaatggagag cgcccgggaa gcgctgaaga
     9061 gtttcttggc tcttctatga ataaacttct tctcgcaaag agcaatgctc ccgagcatat
     9121 cgttgcaggc gagtacagcg gcggctgaca aggccgacac tccttatgga actttctttt
     9181 ttgccggaag aaggtgtttt gaatcgggaa acctcatctg tctggcatat cggcacggca
     9241 ggatggagct atcccgattg gaaaggaatc gtatatcctc cgtccggcgt ccggggcaat
     9301 cagaatcttg ccgccctcag ccgtctcgtg gatgctgtcg aaattaacgt gactttttac
     9361 cgccctgtgt cgcccggcat ggtgcactcc tggctgaaag cagtggtacc cagaccggcg
     9421 tttaaattta cggcgaaagc gccctctctg ctgacccata cgcgttccgc agttcccgac
     9481 aaaaaagtgg catgcacatt ttctgaaagt atgcgccctt tgcgggagtc cggaaaactg
     9541 ggtgcattgc tttttcagtt tccctggagc tttaagcgaa cccgggaaaa ccgcagttat
     9601 ttgagtcagc ttctggatct ttttgaagaa ttgcctctgt gtgtggaggt gcggcacagt
     9661 tcctgggatc atcccgattt tttcagagga ctttccgaga gaaaagtcgc tttctgtaat
     9721 attgatcagc ctcttctgga tgcatgtatt gcgccgtcgg cgcgtgttac cgcagatttt
     9781 ggatatgtgc ggcttcacgg acgaaatacg gcacagtggt ttgacgggac agccggacgg
     9841 gacgggcgct acaattatct ctatacgccg gaggagctgg cggagtgggt ggaacgcatt
     9901 cttgttctgg gtaaaaaagt gaatgacctt tatgtgatca ccaataacca ttaccgggga
     9961 cagtcggtag tgaatgctct ggaaatacag caggcgctgg atttgcctgc ccgtcctgct
    10021 ccggaaacgc tccgtttcct ttatatgcgc ccgtaacagt tgcgcctgtc ttccagaaca
    10081 gtgcaggggc tttgaattca ccgtcgcccc cgtggcaaaa tgcgctgagg ggtaggggat
    10141 agaatggaaa ataccgcatg accatccttg aaaagctcca gcagaactgt cgctgcccga
    10201 tttttctgga cgattttacc agacagctct atgccacaga cgcatcgatt tatcaatgtg
    10261 ttcccagggg tgttgctttc ccgcggtctg cagcggaaac ggctgagctg atgagggcgg
    10321 cggccgctga aaatatacct cttattcctc ggggggcggg gagcgggctg gcaggcggtg
    10381 ctgtgggtga cgggttggtc attgacctgt cccgatacaa ccatttcatt tcagatttta
    10441 atcaggatgc gcagactgtc cgtgtcggtg ctggtgtcgt gctggatcag ctcaatgagc
    10501 atgtggcgcc ttatgggctg accttcggtg ccgatgttgc caccagttcc cgtgcaacgc
    10561 tgggcggcat gattgccaat aacagctccg gtgcccgtgc tccgctgtat ggcgttacaa
    10621 tggatcatgt tgagtcgttg gaagtggtaa cggcggacgg cagggtacac acgctttctg
    10681 cttcatccgg agatatgcgg gaaaaacttg cccgactgca gggcagggtg cttcaggagc
    10741 gtgcggaaat agaggcgcgc tttcatcgtg aaatctgcaa acgctggccg ggctacgggc
    10801 tcgatcggta ccttcgcagc atcctggcag atgccgctga cccggcgtat ctgatcggag
    10861 gcagcgaggg tactctctgt acagtttttt ccgccacatt gaaactggtg ccccttccgc
    10921 cgggtacagg gctgattctt cttttttttg acactgtgca ggaggcgatg gaggctaccg
    10981 ctgcgcttct tgatctgaag ccggcaagtg tggagcacat cgaccgcctt ctttttgatc
    11041 agacccgtgg aaaccgcgct ttcgagcagg cgcgtgcttt gctcgagctg gatgaaaagc
    11101 cctgtgaggg aatcctgctg gtcgaatttt atgatcatat cgaagagaaa ctccagcagg
    11161 tgcagcaacg gaagctggga cgccggagtc tcctttgtaa aactgaaaag gaaaaggcgc
    11221 ttgtctggca tttgcgcaag gcagggttat cgctcctgac ggggtgtgcc ggtgataaaa
    11281 agcccgtggc gggtattgaa gacgttgcgg tacctcccgc acagcttcct cgttatgtcg
    11341 aagcgcttcg ctctgttatc gacaggctcg gtctgaaagc atcgttctac gggcacgccg
    11401 ccgccggact gcttcatgtg cgtcccgtgc ttaacctgcg ccgtgcagaa gacatccgtg
    11461 ccttccgtac tttggcggaa gaggtttccg ctctgacctt ggcattcaaa ggatccttga
    11521 ctgccgagca tggagtcggt atcaaccgta ctgaatttgt caaagaacat atcggcgctc
    11581 ctcttttgaa aataatggaa gaagtgaaag aagcctttga tcccggcggc cttatgaatc
    11641 ccggaaaaat tattgatacc gggcgctggc ggcttgatgc ggatttgcgc atgggcgcgg
    11701 attatgccct tcctgtaccc tttgagccgg tgctgttatt tcgtgcgaaa gatcactctt
    11761 tttcggggaa tctggaacaa tgcaacggct gcggaggatg ccgcaaggca ccgccggcga
    11821 tgtgcccgac ctttcaggcg acaggtgagg aatatttgtc cacccggggg agagccaata
    11881 ttatccgtgc cgtgctggaa ggcaggctgg atccggggaa tgcgccgctc ctcagtgaat
    11941 cactggaggc ggcattaagc agctgccttt cctgtaaggc atgcacgacg gaatgccctt
    12001 ccaatgtgaa catgtcgctt ttaaaagcag aactcatgca tgccagacaa aggcggcatg
    12061 gggttccctt tcatgcccgc cttctgagtc gtgtcgacct tctgggcaag atcggaagcg
    12121 cttttcccgg cactgccaat gcatttcttt cctttgcacc ctttcggcag ctgctgcgta
    12181 aggtcgctca cattgcgccg gagcgtccgc ttcctcttta tgccccggaa cgcttcgacc
    12241 ggtggtttaa aaaacgcacc gcacagaagc cgaagggata cagaggcacc atcctgcttt
    12301 gggacgattg ttttgtccgg cataacgagc cgcatatagg gcatgcggct gtcaggattc
    12361 tggaggccgc cggtttcgtg ccgaagctga tcgaaggaca tgcctgttgt ggtcggcctg
    12421 ctttcagcat gggacgcctt gatctggcac ggaagttcgg cagaaaaaat ctcgatctgc
    12481 tgaaaaaaac agacggacct atactctttc tggagccgtc ctgctatgcc atgttccgtg
    12541 aggaatacgg agagcttgga ctggatgggg cgctgcaggt cgcggaacgg gctgtcctct
    12601 ttgagcagtt tatcgaacag ctgctggaaa aagaaccgga ggcaattccg ttgaaagccc
    12661 tgcctttcgg tttggcaatt cacgcccact gccatgcgaa aatgcttgcc gacacgcaga
    12721 ttcatgcccg gctggcacag cgtctcaccg agcagaatgc cgctatgctc aacagcggct
    12781 gctgcggtat ggcgggatca ttcggagtgc tggaagacaa gtacgcattg agcctcgccg
    12841 tggcagaccc tcttctggca atgatcgacg cactgccggg cgatacgaaa gtaattgctt
    12901 ccggcgccag ctgccgccat cagctggcgg aactgtctgc ccggccgccc ttgcattttg
    12961 ccgaagtatt ggagcaggca ttacgcacct cttcggaaaa ggaactcaga taagcagaga
    13021 gccgatccgg tagaatgcga ttgccagaat gaaggcaaaa gcagtagagt agactgtgga
    13081 aaagagcgcc catttccagg agccgctttc ccgccggatt acggcaatgg tgaccagaca
    13141 gggagcataa agcatgacga ataccataag tgcgaaggca cgtaccggat tccagtcaga
    13201 tcgctttgcc agctgctcgg aaagagacag gtaattttct tcgtctgtgt tttcaaggga
    13261 ataagccgtt cccagaacgc ccagcaccac ttcttttgcg gcgaagcccc cgataagcgc
    13321 tatattggtg cgccagtcaa agcctgctgc ccggctgatc ggctccagtg ctttgcccgc
    13381 acgtccggca taactgtacg ccagtttttc ttccggattc gtttcttcgg cagggtgtgg
    13441 aaaatacatg agcgcccaga gcacaagatt gatggcgaga ataacggttc ccgctttttt
    13501 catgtacatc caggtgcgtt cccaggtatg ccgtaaaacg ccacgcagta cgggcatgtg
    13561 atagggaggc agctccatga caaagggcat ctgctcgccc cgcaccagcg tgcgccgcag
    13621 tatccacgct gccgccagtg ctgtcagcca ggaaagagcc cagagaatca tcatgaccag
    13681 cgtgcggtta ttcgggaaaa aagccgctat cagcatggca tagacgggca tcttcgcacc
    13741 gcagttcata aagggggtca ccagcatggt caccaggcga tccctgtctt cgcgcatggt
    13801 tctggtggac ataattcccg ggacagcaca tcctccggca cccaatcctc cagcaacaat
    13861 aaaagccagg atacttttcc cctgaagtcc gaaaatgcgc agaggacgat caagaataaa
    13921 agcaacccgg gcaatatagc cactatcttc cagcgccgcc acgaacagaa acatgaaaaa
    13981 aatcagtggc acaaaactca tgacaccgcc cacaccgccg ataatcccgt catttaaaag
    14041 agagtgcagc ataggcatgc tgtttttcaa cggagcgatc aaagcggcaa gagagagaaa
    14101 tagagattcc atgaaatggg tagggctttg aaaaccgccg aaccagggaa tccacttcca
    14161 ttcgtcggat actttgaaga cccagaaaaa aagtgccgcc accaccagca gcaatataat
    14221 cggtccgata atgcggttgc agataatcag atcaagctgc tcggtaatat cccggcgtgc
    14281 ttttgcgtta aaagtaacgc acctttttac cagccccgaa gcaatgccat aacggtgttc
    14341 ggctaccaat gtggaggcat cggcattgaa atgccgttca atcctgcgga cggcatcttc
    14401 tatttttaag aagagttctg ctgtttcttc cggcgcacgt ttttttattt cttcaacagc
    14461 gcgctcgtct ttctccagta cttttacagc aagccacgcc ggcgacagat gggtgttcag
    14521 agcgggaatt ttccggatct cttccgtgag cgccagtact tcatcatcga cgccatgccc
    14581 gtaggataaa ggggcgggac gggcctgttc tttgtccaga agaatcgcca tactcgccgc
    14641 agcaatttca tcgatcccct tgccgcgaca ggcgatggtg ggaatgacag gaacgccgag
    14701 aaggcgggac agagtttccg catcgatttt atagccgcga cgttcggccg tatccaccat
    14761 gttcagagcg attaccatgg gaataccgat ttccatcagc tggatggaca gatacaggtt
    14821 acgctcaaga ttggacgcat ccacgacgtt gacgatgaca gccgggtgct cgtccagaat
    14881 gtagttgcgt gcaacaagct cttcctggct gtacgcagtg agtgaataag tgccgggcag
    14941 atcaacaaca gtgacacgat gcccctgcca gcgcatgaca ctgcttttaa actcgacagt
    15001 cacacccgga taattgctga cctgctgtgc cgagccggta agcgcattga acaggcaggt
    15061 tttccctgca ttcggattgc ctgctataac tattttcccg ggagacatgg agaagaacta
    15121 tccttcagat acaagatggg taagtaaaaa aggcagggaa gagaggggaa ggactcagtc
    15181 cagagaactt tctgatgctg ccagaagaga ttcggagctg cttttaatct gctcaacttc
    15241 aatcgtccgg gcttcactca tgcgaagcgc cagaaaatac cctttcacaa aaatctcgac
    15301 cgggtcgccc agcggcgcat ggcgttccac gcgcagttct gcaccttttg taatgcccat
    15361 atcaagaaga cgacgccgta aactcagcgg agtatcggtg ccgatgccca gaattttagc
    15421 agtgtcacct gtttttaaat caaacaatgt atgttttatg acctgtctcc ttgtaccatc
    15481 ccggacgtgc ccaatggaag ggcggacttg ggatccgcct tctgtgccga agcacactgc
    15541 tccttcaatt cttccgggag cgagcatgtg gagcacccgc agcaggggga ctggtcccgg
    15601 cgcattgaac ggatgccatg gcgcaccaga agggtgaaag cgaggagcac aataaaaaga
    15661 acaatgagta attccacgat aaacactacc ttatgtttga ccgctgcctc ccacaaggtt
    15721 cacgaatgtg ttcacaagtt aactatacta tacaaacacc gccaaaacca aataattccc
    15781 cctaaaacca tgccttttcc ttcggagcaa gcgatgtctg catcgaaaaa caacagtaca
    15841 gatccgaagt gtaaaagacc ggggaagcag aaccgggcac cgttcgagct gtacaaagga
    15901 gaccgggccg tcatccgggc agatggcacg ttccttcaaa aaacagcaca ggcgctttaa
    15961 tgcccggata aattgacctt cttgggatgc gacaggtaaa ataacatctg taatttccct
    16021 gtcttttcag tgcttcaatt taacgatctg gcgaaagcat atctatcttg gctcgtattt
    16081 cttcttttgc ggtgctgttg cgcatcgccc tattcgctgt atttctcagt tgtgccttta
    16141 ttgccggttc ctatctgggg aaaaaatccg tgccggaaat gccgtcgctg acagcgcttc
    16201 ctgcacatca ggatacattg ccggaagggg gcgagcagag cggagagcat gcacttcccc
    16261 tgcttcacag cagtgcacag gcgccacaac tgttcttttt tgccggtaaa gcctcagatg
    16321 acgtgtggcc ggtggttgcg gaagaggtga ccttggccaa ggaagcgggg atttccaatt
    16381 atgtggttcc tgttgcgctg gactggtcgg agccttacca gcccgaaaaa gcgtccgcca
    16441 ttgagaagca gttgcagcag tatctggata ttaacagccg cgcgcagctc cttctttgga
    16501 tcaatctgaa tccgcccgcc tcctggctga ccctttatcc ggatgccgca ctgcgctttc
    16561 agtcggggct gcaggcttat ccctccatcg ggtctgaatt gtggcggaac cgtgcactgg
    16621 aaatccttgc cggtgaaata gataaactgg aaaagagcgg ccttgcggat catatacggg
    16681 gatatgtttt aggcgctttg ggagagcagc gctggcggct tccgggcgag cacgatctat
    16741 ctgaggcaaa ccagagtgcg tttcggcgct ggctgcaaaa tcattatgga actgaagagg
    16801 aactggcgaa ggcgtggggc gtggatgcac agacttttga tcgatgtgcg gtgcctgttt
    16861 ttagccagca ggagggacag gaaaaacaag ctcctgtttt tcttgtgctg ccgcagatgc
    16921 agcccatgat ggattattac cgctactgtt cagatcaggt ggcggatacg ttaattagtg
    16981 ctgttactcg gataaaagaa attgcccggg acaatgtgct tgttctggcg ccttatgggt
    17041 ttacttatga actcactgaa ccatcttccg gtcattatgc gctggagcgg cttctgcaca
    17101 gtgatctgga tggcgtcatt ctccctgtgt cttatgttga ccgcgggctt ggcggtaccg
    17161 gcgggcctgt cggtcctgtg gacagcctgc ttgcccgggg caaaacctgc ttcattatgg
    17221 atgacacccg aaccggtgtg gagcgtgacg aaagcacggg gctattcggc agagtcaaag
    17281 gtatccgccc cgatgacatt tttgaagttc agaaacgaaa tttcgcattg gcgctcagct
    17341 gtggcatggg gctgatctgg actgatccgg aatcgcaggg ctggctgaat atccctgatc
    17401 agtggaaaca gtttgccaga ttaaaaacac tttacgcgga ctaccagaac aaagaattgc
    17461 agggacagac accggaatct ccggcgactt tgactgtggt ggttgatgag cgggcaaact
    17521 tctgtcttgc ttccggcgac agaattaaca atgcgcttct gctgaaaggg cgggagatgg
    17581 cattgcgggc aggcgtgagc acccgttttc atcttttaaa agatgtggtg gacggcgtgg
    17641 cgccgccttc acctgtatac ctttttctaa accctttcat gatcacagaa gaagaacgca
    17701 atctgttgca cgcccgactg gcaggcgaag aagcgtgtgc catctggctt tatgcgccgg
    17761 ggtacttcgg agcggagtcc gggatggaag gtatgtccag actggtggga atggatatcc
    17821 agccctttaa agagcctgct tccggtgaat ccgtgtatat tctgtcgggc ctgtatatgc
    17881 cgcctgatca gcctttcggc tcgaagaccc tgtggaaccc gcttttcttt ataccgcccc
    17941 atgacgatct ggatcttctg gcacactaca cctcggataa agataagggc agcattgcca
    18001 taaggacttt tccggagaac tggacatctc tttttatcgc tactcccgag ctgagccccg
    18061 cactgctgag ggaattgatg gtgctgctgg aacagccgat cttaaccaat gacgctgaag
    18121 atatctggta tgacaccgtt tatgcacgaa ataatcttat gaccatccac agcaatcagg
    18181 ccgggcgccg ttctttctct ttcggtcagt attatgatat cgaagatctt tttgattcat
    18241 ccatgtcctg gtttcaaaaa gacacaattc tgctgaccat gcgtacgggc gagacgcgtg
    18301 ttcttcagaa aaaaataata gagaccgaga aacgatgatg gcaggcagcg ccatgccgac
    18361 gcctgttctt caatatttct gaatgcctga tttcaattta cccggatgaa cagagaaaga
    18421 agagaggaac catgctgaat gtggaccgca gatccgctct ctgccgtttc attttttacg
    18481 gctggctctt ttttctggtt cttgcgctga ttccctggac cgcaaatcct gtcaagccga
    18541 ttaaagacct ggtcacagca gcagcagctc ttgccgtctg tgctgccggg atcgggctgc
    18601 tgccccgcac tgtcggtgat atgcggaaaa aactctcgcc gcccctctgt attctgggac
    18661 tgtggctttg ctggtctgtc gtttcggcat tggcgtcacg gcatcactgg cttgcggtgg
    18721 atgaactccg cattcttata ccgtggcttg ttctgtcatt ttatgcttct cttctgtttc
    18781 acagtgggca aagcagaaag acgcttgcgc tggtgatttg cctggctgtc tgcctttcca
    18841 gtttatatgg actctgccag tatctgggct tcgatccatt tccctgggtt acccgggaca
    18901 tagaggagta ccgggggctt cccgccacct tcggcaaccc gaattttgca ggacacagcc
    18961 ttattctcgt tatcatagtg gcggcgggtc ttatcgctgc ggcaccccgc agtgcggcct
    19021 ggctgcttct tccgctgccg ttgtttcacc tttaccagac gcatatgcgc ggcgcattgc
    19081 ttgctttggg cgctgccact ctgctggttc ttctctatgt ctttttccgc aacagaccgg
    19141 gcagctccct gaagaaaata tcgtgccttt ttgctgtact gctgctgatt gctctggcag
    19201 ggctcgcctt tctgctgcct ctctccaaaa agatgtccgg atactatttt ccaacgggtg
    19261 gctctctgat tctgcgctac aacggctatt acggtgctgc ccgtatggca ctcgatcatc
    19321 cgattttagg tgtaggcccc gggaattaca gcccggagaa tataccttac tggacttttt
    19381 atgaaagacg gtggttcgcc gaccagaaca agcggaatac acacgtgcac tgtgatttgc
    19441 ttgaaagcgc tgtggagacc gggtttcccg gcatgattct tcatcttctt ctttttattc
    19501 tcctgattgt gcaatcgtta tacgttgctt ttgaatccgg agagctcgaa aaacggcggt
    19561 ggggagtgat ttttgcagca tttttcctgg catttttggt ggatggactt tttggattca
    19621 acctgcgcat ccctgtttca ggagcacttt tttatctcgc tgtcggtgtt ttcagtgcct
    19681 tctggcatga aagtaccgag aataatcagg gaaggcagga acgcccgtcc ccgtcagcag
    19741 tgcccgacag agggcgcctt ccggagcttc ctccacaaaa aaggcttttt atggggctca
    19801 tcagcatagg gcttgtgtcg gtgtctctgg gctgtgcctg gtgggcgctt cgggtttttc
    19861 agggggaact ggactggtac cgcgggaaca gtgctcttgc ctgggcggaa agaaattccg
    19921 gagatgcgca agtgcgcccg gtgcttttaa aagcggaatc tgctgctgcc cgtggtttta
    19981 aaaaatgctt ctgggacacg cggtacagta atttactggg gcatattgct ctgaaaaaag
    20041 gcgataccga tcaggcaatt gtttattaca cccattcgca tgagatggat ccgtatcatc
    20101 cgcatacggc tgccaatctg gcacgggcgt ggctcagccg gagcatgcag acgatgggga
    20161 gagagagttc gctcgctgat ctggaaaaga atgcgcaagg gctggaaacg gcagaaaagc
    20221 atgcgcgtta tgcagccgtt tattgccctg aatctcctta tgtgcaggat gtcctctggc
    20281 ggattgtccg tgcagaagca cagcttgcag aaatgagagg cgaggatgcg gtgcctcttt
    20341 ggaaagaaac ccgggatctt attaatgtgc atctgcggga tgttcctctg gatttgcctg
    20401 ccgcccgccg tgcgttgttt tctgcctgta tcaaacttga tgacctcgat accgttgaat
    20461 ctgttttgac ccctcttgcc gaagatcagt ctttgtcgtc ggagttctgg gagttttaca
    20521 gacgttttgc ccgggaaaaa ggacagatgg atcactatct ggatgccctg ttgaagcagt
    20581 gcgcaagggc gttgtcggag aaatcagatc gctttgttct tctctttggt taccttagtg
    20641 ctgcctgggc agaagtatat acggcggaag gaggactgga tttttacata gacgaggcgt
    20701 tgcgccattt ccccgagagc ctggcattgt gggcacttgc gatccgcgct gtgccgcctg
    20761 aagaagcggg ggcggtactg aaagaaaaaa caaacaggac aggaagcagt tcctgtccgg
    20821 agttcattaa gttgtttgcc gctgaacccc aggacgaaaa aagtgtgact caatcagctt
    20881 cgcttcttgc ccgggaagcc atgcaaggcg gtgacagctc tgtgattgga gaccttgcag
    20941 tttttgctga tgtccttttg cgcaggacag agtcgcttgc tcttgaatcg ggcgcaaagg
    21001 cgggcattta tgccaatctg gctactgttt atctggaagg tgctttgtgg gaagaagcgg
    21061 acagatattt tgcacaggca atagccctgg ttcctcctgc ctcttcagca cggcttaaag
    21121 cctcccgatc ccgggcgctg gagcagctgg gcagattgcc tgaagcgctg cagctcagcc
    21181 gggaggcgtg gcagcaaatg cccgacgatc cgctggtgct cctgatccac gggcgcattc
    21241 ttcgcctgac aaaacagaat gcggaggcgg ctgcgctgct tgaaaaggct gccgggcgat
    21301 ttccggccgg atctcctctg cagcggcagg cacactatga gcgggcgctt gcagaacaac
    21361 acacagcagc accggcagcg gggaaggggg agtgatatga ataaaacgcg gatcagggag
    21421 aaagaagatt ctgcaccggt gtcggacaca gaaaaaataa aagtgcctgc ttccctgttt
    21481 tcttttacag gagtcctgtg cattatttca atgatcggaa tactgggaat gcttctgtat
    21541 ttttcagaat cgggtgaagc atttctgtct gcccgcgatg attctgttgg tgaggtattg
    21601 atgaagcggg gagaccggct ggtgcgggca ggaagtgata aggaagctct ggcttattat
    21661 gagaaagcac tggcggcccg ttttgcaggt gctcaaaata aaacgcatac ctttgaagca
    21721 gcaggtctga ttctctggaa gcagggacac tttgaccggg caaaggacta tctgagcaga
    21781 tcgcttcaag ggtatcagcc gacagaagaa ccttatgaag ccctgattga tattctgctt
    21841 aaagaaggac atgcggaaga ggcagcgtcg ctgctggaag actggcggtc ggttcgggat
    21901 gaagcggcag atccgtggca gagcgataaa gtgctgctgg cttcaggaaa agcagcggct
    21961 gcgctcggca aagaggagga ggcgctccgg ctttttcagc aaggagctga gcagggaggc
    22021 gccccggaat tctctgcctg cctggcactg tattatgcga aagcaggaga ccgtgaaaaa
    22081 gcgatctcct gcttgaaacg ttattttctc agaggcggcc agggggaaaa gttccgggcg
    22141 ctttatgatg ctcttctttc agataaacca aatccggatt cagaattgct gtctcttttt
    22201 acgcattgat cttctgcgtg cgcccggtca acaatcagca ggctgctgtc cgtttctgta
    22261 ccggcgcagc aggaagtatt caaaactgtc cagcagcgcc acataggacg cagtaataat
    22321 attttctgaa acgccgactg tggaccagct gtctccggga tcagaagtct caatgagcac
    22381 tcgggtttta gcctgagttg ccagtttccc atcaagaatg cgcaccttat aatcttccag
    22441 atgcatttct tcaatagcgg ggtaatggga tttcagggct ttgcgcagtg cattgttcat
    22501 ggcatcgaca ggaccgtcac cttctgcgac cgtatggaca atttcaccgc ccggcagttc
    22561 aagttttaca gtcacttctg aaagggtcgg cccgtccgta cgcggctgat tgacccgtgc
    22621 atggtagccc agtgttttaa agagaaacgg cgtctgattc agcacgcggc ggatgatcag
    22681 aaggagcgaa gcgtccgctc cttcgaactc atagcctttg ttctcaagtt ctttgatctg
    22741 gcgcagaatt tcgctggtat gcgggctgtc cttttccaga tggatgccca gttcttccgc
    22801 tttctgaatc aggctggatc tgccggcaag ttcactgact gcgatacggg taaaattgcc
    22861 gaccagttca ggttttacat gttcataact ggttttgttt ttctttaccg catcggcatg
    22921 catgccgccc ttatgcgtga aggcattttt tccgacatac ggcgcatgat cgcgaagtgc
    22981 catgtttgca agttccgcaa taaaatggga gcgacgggtc aattgtgcca gctgctcggc
    23041 agagatgacg tcatagccca tcttcaattg cagtccggga attacagtac aaagatttac
    23101 attgccgcat cgttcgccat aaccgttcat ggttccctga acctgttccg ctccggcttt
    23161 gaccgaggca aaaacgctgg cgacagccgt gccgctgtca ttgtgcggat gaatgcccac
    23221 ctgagtgccc ggtatccgcg caacggctgc acgggtcatc tccgcaatgg cgtccggcag
    23281 ctgtccgccg tttgtatcgc agagaacgac agattccgcg cctgcttcgg ctgcacattg
    23341 aagcacagtc agtgcatatt ccggatcttc cagataaccg tcaaagaaat gttctgcatc
    23401 cagaaaaaca cgtcggccat tggctttcag ccaggcgaca gattcggaga ccagtttaag
    23461 attgtcttcc agcgagatcc gcaggacatc cttgacctga gagacgatgc tcttcgcaaa
    23521 aatagtgact accgggcagc ctgtctgaat aagcgctttc agattcggat cctcgtcaca
    23581 actgtatttg atgtggcgcg tgctgccgaa agcggccata acactgtgtt tcaggtcaag
    23641 atctttgacc tgctcaaaaa gcattgctgc cttggggttg gagcccggct gaccgccttc
    23701 aataaagtca atgcccagcg catccagcgt tttgactacc agaagctggt cttctgatga
    23761 aaatttgatg cccggaccct gggcaccgtc tcttaacgtt gtatcgtaaa taaagatttt
    23821 tttcatggtt tatgtgcttc ctaatacaat actcccctga tcggatgttg gaaaagtcgt
    23881 gtgatcctct gcaaaatgag ccacacgccg catagtataa caaaaatcgg gccggactgc
    23941 ctaacgccgg attcagaaag agagcgtttc ttctttcaga aagtcaacgg tgaggcggtg
    24001 cccgtcagga atcatgatgc cgctggacaa ggcggcgcct tctccataag cggcagattc
    24061 tcccggctga atacccggac cgcagagtgc aaagggtaca gctccggatg catgggtctt
    24121 ggtactgatg agggtaaaat ggtcgggcgc aaccagtata cggcagtctt ttctgtctgc
    24181 tgcataagcg atgacaggcg ccacaacttt cgcgtcaaaa tcttctatcg cctgaatttt
    24241 caaaggaata ctgccctgat gtgcggcttc atcaggtgct tccacatgga ggtatacaaa
    24301 atcatgttct tccagagcat taagcgccgc tgccactttc cccgcataat tggtatcgat
    24361 ccagccggtg gcgccttcca catgaagcac ttcaagcccc gcgcagacac ctatgccctt
    24421 gaccagatcg acggcagaga tcacggcgcc actgacaccg aagcgatccc ggtaggattc
    24481 cagcgtaagc gcccgcccct gaccccaggg ccacagagat actgccggaa gtttaccgga
    24541 ggcgatgcgc gccacattga caggatgctc ctgtaatgct ttttgtgatg cctgcatgag
    24601 tgcgataagc agatcggcag cagcgccctg aggcaggaag ggctcgaagg attgcccagt
    24661 aatattatgc ggaggttcgc ataccgcctg cgccagatct tcgaccgtgc agccgctgtc
    24721 agctttcagt atgcctgtat gacgatagct gacgccgcaa tggagacgga tcgggaaaga
    24781 ggcggagagt tccttatcca gatgattgat taattccgac gcttcttcag tggtgatgtg
    24841 accggctgta aagtctttca ttacgccgtc cgacagcgta acaagattgc agcggaaggc
    24901 aacttcatcg gcggcaagag tgatgccctg gcttgccgct tcgatcgccg cccgcccgga
    24961 aaaggaaaca cggggatcat acccgaacag agaaagattg cctacatcac tgcccggagg
    25021 catcccttcg gggatggggc agaaaaggcc ggtcactccc atacgggcaa cacgatccat
    25081 gttgggcgta tgagcggctt cgatcggtgt tctgccctgg cacgcatcca gaggatggtc
    25141 agccatgccg tcaccaagca gaatgatgta tttcattgaa aacttctccc ggtctgccgg
    25201 agcactgttc ttaactggaa tgagcgtgag atcccccggc gactcgtata cggtaccatg
    25261 ataacgctgt taagagcaag aagattcctg cgtagccatc cgatcacatc agaactgcag
    25321 gcgaagtacc gggataaaaa agagcgacgg caactgaagt tgccgccgca aaattacacg
    25381 atcagaaata agtctgagtt tatcagatgg gggtgctgtc cgcgtctgca gctgtaccgg
    25441 tggcgaagcg aatgataccg ctggcatcta cgaagaaata gcggataccg gttacgccat
    25501 cattgttggg gttagctgtg cacgtgaagt tctggaaaag accattagca tcggcttcag
    25561 caccagccaa attgtaatca tagccctgca gggtgtcgtc attggctgcg atttcaagat
    25621 ccaggaagga aggtccgtct gtaggttctc tcagatcgtc ccatgcatca gcatagcctt
    25681 tggtggcagc ggcaaaggcg gtctgcgcac cgacgatggt cttcaggttg ccgatagcgg
    25741 cggcttcgtt ggactgcagt ctggaacgaa gcaggttggg gatggcgatg gctgcgataa
    25801 tggcaataat ggccaccaca atcatcagtt cgatcagcgt aaagcctttg ttcttcatag
    25861 cgatacctcc atgttggttg tttggaacaa agcacacaca aacaaaaaac tgtctaccta
    25921 atttatgagc aaatgttgtg ccaaagttat tttaatgcaa tacttgaaga tattcgcagt
    25981 attcgcatga aatgtgctgc agaagatagg ggaaattgat cctgctttac aaaaaaacgg
    26041 cacaaattac aaattgttgt caaaaaagag cctccctggc agggctattg aattgcaagc
    26101 atgcgctgaa tgggcaggag tgcctgttgt cggattgttt cattaagatg aacctgatac
    26161 tggcattgtt tcaatgccgc cagcgtatct tcaagggtta tataattcat gtgcttgcac
    26221 cgtaacgtgc aggcccgcac aagacgtctg tggggaaatt cagcggcgag gttgtcaccc
    26281 atggaacact cagtgagaag agcataccag ggagcgtctt tgtcacgtac atagtcgacc
    26341 atagccttgg tgctgcctga tttgtcaacc agttgaatta cttccggcgg gcattcagga
    26401 tgggcgagga tgacggcgtc ctgatactgt tttcgtatgt ttgccacgtc ttctgtggta
    26461 aaaagttcat ggacttcaca cttggcatgc cagccgatga ccagtggctg atcagccgga
    26521 acagcgtcaa atgctttctg atccgcttcg gaatcgagga aggggagccc cagttgcgtc
    26581 gctgtatttt ccgccagata ggcatcgggc agaaagatga tgcgcttgtg cccgtcttcc
    26641 acaagtttgg acagcacttt gtctgcattc cccgaagtgc agcagtaatc ggattccgct
    26701 ttggaagtgg cgtaagtatt cacataagta accactacag cgccggggaa ttttgctttc
    26761 agcgcacgga tatcgtcagc gctgatacct tctgccagag agcagcccgc tttttctgag
    26821 gggacgagca cggttttctg ggggttgagt attttggctg tttcacccat gaaccagacc
    26881 ccgcagaaaa caatgatatc tgctgtcgtt ttggcagcga tggaggaaag ctgcagtgag
    26941 tcgcctgtgt agtccggaat actgtgataa agtgccggct ccatataatt gtgtccgaga
    27001 atcacagcat tcatctcatg cttcaggcga ttgatttcat gcgcgatttc caccttggct
    27061 tcaatttcaa tgtcgggaac gatgccctga agccgttctt tcataaattt tctggtttca
    27121 tccagacctg cctgagccga caacgtattc atgaggaaat tcttctcttt ctggccgggt
    27181 gttaaaacaa gggggttgac aatacggttg gatcttcatt gtaacaggag aaatatgtgc
    27241 aagtaaaaga aagcataccc gggctgctta gtgaaacgat gtaaaagcct tatgtactcc
    27301 cggaatactg aaaattcact ttcataactt actatttatt ttgagcagtt ttcagaaaaa
    27361 aacagcggca cagtcctgct caggattttc cttatgactg tgctatact
//
LOCUS       DAOPWB010000027        27364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1737_length_27364_cov_5.772126, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000027 DAOPWB010000000
VERSION     DAOPWB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 27364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27364
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1737_length_27364_cov_5.772126"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PKN92_04155"
     CDS             <1..810
                     /locus_tag="PKN92_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNZ48018.1"
                     /translation="LLGGLLSLDLITLSDGKYQNTEASSACLVTGGAAYQGNLMQHTM
                     NGRDAWASLTACIQSGTCQIDVQRRSGEALKSFILGMRNIAQMSARECLAAVDLSGFR
                     HMLDLAGGPGTYGITFQQQFPELHVTLFDRPEVVAIAREQVSEAGLSSRFSFIGGDCI
                     CDDLGNGYDLVFLSNIIHSFGTEENAGLMRRAYDALVPGGTLIIKDFILDNDRQGPAY
                     GLMFALQMLVHTTAGNTYSFEEIQRWTDAAGFRQGESISLTPQTRLWIARK"
     gene            874..1095
                     /locus_tag="PKN92_04160"
     CDS             874..1095
                     /locus_tag="PKN92_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48019.1"
                     /translation="MLYFKTAQYIPGKGDAWTYYECDENQTIVRQLTHIPETGDIDRI
                     PDPIVKKLYRPERLLPAEAQEFQELWGEG"
     gene            1102..1974
                     /locus_tag="PKN92_04165"
     CDS             1102..1974
                     /locus_tag="PKN92_04165"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNZ48020.1"
                     /translation="MSLISPYQSLADRQFKGNLHAHSTLSDGIFSPQEVIDLYANNCY
                     DFLMLSDHDILSNPADWKDRGMILIPGNEITANGCHLLHVNAHELIEPNPDRQQVLNA
                     IQRSGGFAILNHPNWGFNFCHWPQAELERLRGYAGIEIYNGVTERAEGNAQATDRWDM
                     LLSLGRKVWGYGNDDCHISADMAIVWNMVFAEERSAEALLRALEAGQFYVSTGVRLER
                     ISQCGNRVHISSPDTVSYRLIGDYGTIFATAEGPELDFEADPTLPFTYFRIECFGKGH
                     SRAWTQPFLLQAQA"
     gene            complement(2027..2932)
                     /locus_tag="PKN92_04170"
     CDS             complement(2027..2932)
                     /locus_tag="PKN92_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ48021.1"
                     /translation="MDHSSFSLSRRQFIGGAAALSAAALSLHALPASAEKPDSKFNGV
                     QIGVITYSFRSLPCSAEELLGYITRCGISSVELMGDAAEDFAKRHTPDTGVGGPMDGF
                     EALRKLYNEAGVNIHIVKFGHIGDEKMSDAEMGYYFAVAKALGARGITREMPDKSGLA
                     ERLGPVADRHEIMIGFHNHTQITPDSYTGDMLSYGRYLGINLDIGHYVAGTGEPAIPM
                     IDKHRDRILSLHVKDRKKKKLFSDGPNMPFGEGDTQVAEVLQFLKQEGLTFPADIELE
                     YDIPEGSDAVKEVTRCVQFCKEALA"
     gene            complement(2987..3910)
                     /locus_tag="PKN92_04175"
     CDS             complement(2987..3910)
                     /locus_tag="PKN92_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNZ48022.1"
                     /translation="MEMKRRSFLTAAAAGAGMWSALGGSSAQAAVPEGKPFSKLPLKI
                     SCSYGWLSDCLPKGNRPIDVFRLAAEWGFPAVELLGAGEDKKNYDAYAAASQETGVAW
                     SCIMGAGRITQGEMLDLSQHDRLEEQCAAAIEQAKGLNCKCIVGLTGETSSKWTITYD
                     KAAQNVIKFLRRVKGMLEDNGITMVLETLNQLRDHQNFWLTTTDQAQMIVQAVGSPNI
                     KQLYDIYHQQISEGNLIPNIKDNIEGIGHIHIGDNPGRKQPGTGEINYPNVFKAIKET
                     GYSGYLAVECGYTDGPENAIQDLLKCFDGWA"
     gene            complement(4064..4657)
                     /locus_tag="PKN92_04180"
     CDS             complement(4064..4657)
                     /locus_tag="PKN92_04180"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ48023.1"
                     /translation="MKYVLFFLIAVSLVFMGTGVFASEDCSRILLFNGKNLDGWTLYQ
                     CEAKADGEDLLIESGNGMVQSAEQYGDFIWEFEWKALAEDDWDSGFYFRYTEIPEGAP
                     WPPRYQANLRKGLEGNVSELKGASSTGLIKERDWNQFKLTVQGTKAALEINGKPAWEA
                     EGLEGPEKGYVAFQAEVPGGGKHRFRNVYLTPLDKKD"
     gene            complement(4685..6781)
                     /locus_tag="PKN92_04185"
     CDS             complement(4685..6781)
                     /locus_tag="PKN92_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48024.1"
                     /translation="MSRLLVLLLFGLSGVCAAQEASVQFRVEGKVLLCHAGNRELARA
                     DIPSVNDRVPVVRFDQWGKWVRVHLRWELEEAVEQNDLFMPFHLQFDPNFWWAPHLTP
                     EEGLVIAQHVFRSPAVIAASEEGVLALVPDLDMCGKDTAAPWYMDFNAPEKYFGLGMS
                     RTEVPEHVLFKKVPGMTLGTGSVDLSFYLAWLDGKEDIFNPWQSVAAFLWDRWGKALY
                     DRGEPTTVSPERYVEHTYGWAFDRWAHAVWQEFDLHGTRVGSPVFIVTVTDSPNYPGH
                     ADQREFPSIWNQAWFSSLRAASGVFRYGRRTGNEDLMRRARLTEEFTLAAPLDKGVFP
                     TVYRSDVERVTIDGVTVHRPKGWDVGYWVNSNRVPWEHGITDRWYNVLDMSWTALLLL
                     RWHEEIEPDERILPYVSTYAERLLQLQDEDGFFPSWLDPETQKPSAVLAQSPQTSMSV
                     TFLLKLAQMTGEKKYETAALRALDAVIREIIPAGQWEDYETYWSCCRLGQDHLGRRFE
                     RNAMFKQNTLSMFWTSEALLEAWRISGREEYLSWGRRTLDELSMVQQVWQPPFIYVPA
                     LGGWGVMNMDGEWNDSRQTLFAELFIEYGKVLGEQRYIERGIAAMKSGFVMMYCPENP
                     AVKKLWEKVWTFFGTEDYGFMMENYGHGGVTTPDGEGMGNFTIYTWGNGAASEAWSRI
                     LDHHPELFPACQPEAE"
     gene            7001..7903
                     /locus_tag="PKN92_04190"
     CDS             7001..7903
                     /locus_tag="PKN92_04190"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNZ48025.1"
                     /translation="MLYMLITLVLITAPDIHAMNPLTPEEEYVIVHKGTERPFSGKYN
                     RHFEKGVYHCRRCSAPLFNSSSKFEAGCGWPSFDEALPGAVQWIPDADGRRTEILCAR
                     CGAHLGHVFQGEGFTPKNTRHCVNSVSLDFQKIPATSQEHRAVFAGGCFWGVEYYFQS
                     AEGVLSTRVGYTGGHTEFPDYEQVCRGTTGHVEAIEILYDPARTDFEKLARLFFEIHD
                     PTQTNGQGPDQGEQYRSVIFYQNEEQRTIAEKLIAHLRAKGFAVATALEPASAFWPAE
                     DYHQRYYERRRGTPYCHIRTPRFE"
     gene            8002..11169
                     /locus_tag="PKN92_04195"
     CDS             8002..11169
                     /locus_tag="PKN92_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ48026.1"
                     /translation="MSASRKQLNLDGKWFFAADPSDCGRKEQWFERFPDDTDEIYPPF
                     LSGQLPGDGGIGWYQHSFAAGPSWSQSSTFLHFERASYFTEIWLNGHYLGSHEGAWLP
                     FSVPTEEHLHPDSNTLVLRIVFPGQHAGDADLNPASLPYYGETGSTGGLLGAVSLLAY
                     PSAHIDSIFVQPDLRRARLAVDVSCTGGTAKTEIVLRVKDTDYSVTGKPGALLLPMPD
                     FQAWTPEKPHLYRLEVLLKDGRKTVDAEALSFGMREFTLKDNRFHLNQRPLFIKARAC
                     FAAPWLSLEKTARLQTFRQFIAESRDAGFNMITLTGHPVPEDFLCVADEEGMLLSVES
                     SLRPMTASDRLEQRCEDAVTDMIRNGRNHASLVLWRLFNEERTRAAETGNAARALSDR
                     LFTLARELDPSRLIFAGGAHADSSDAKLLRPYRSTPEPYDDLFFCPTDPVPENRTRYL
                     QCCGAAGKLAIISGMTSFQTAEHSEEDDTLHADFDQRGLNRIFGSLAAFNDLTDESAK
                     TFVAAQIQAMRANSKIAGYFHAFPESSGCTSEKPNALEIVAAAQAPLLPLISLEKSNL
                     HPREATGVTIQMIGDPGQGGSADLSLQVIGPTGQVLWKKKRHTKLPRHGQLLWSGSIG
                     GSGALGLHRFVASLMQGTRVLGRGEDSFFVFEQAAPSPVEVHLLDPEKIYLDRVAALA
                     KTGNLLAPLHIIPPLSNTVRAYPDNDLMQILAQVEEGAVAIVFSPPEDWNDLFSILDA
                     KISATPRPASCSWASAFHYVKLHPVFEKLPSRCLMREVYKNVASALNFEEQGEEDISG
                     SLCRTADALTGESTARWGNNILVQRYGTGRIVMTCMPVLEHLGKDPVADRLFVNLLNH
                     FERRSIPSASPVPPNQRAVEWLREEKKTQLRRWLILGGIDLSSDENRKKSLHSIEMER
                     NFEAAVEGWYRPEKWRTWFTRADADHEVPLTDALDPGGFFFSHYERSASYAYTEITCE
                     RRLSSYLRVRFHSPIRIWVNQVPVFESEGPPDGENYCIESVNATLKHGKNTLLVKCSK
                     ERGKALFALDFVSDDHLPSALKWWK"
     gene            11221..12090
                     /locus_tag="PKN92_04200"
     CDS             11221..12090
                     /locus_tag="PKN92_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ48027.1"
                     /translation="MDEPSLEKGVSLWEDAWRRLRKNKLALAGLVMLMVVLLLSVAGP
                     WCSPYGYETQDTALGAAAPSLRHWLGTDMLGRDLLTRLLHGGRVSLGIGLSATLVSLL
                     IGVLYGALSGYAGGYTDRVMMRIVDILYALPFTIFVIILMVFFGRNFIFLFIAIGAVE
                     WLTTARIARGQTLSLRHREFVEAAVILGYSRMRILLRHIIPNMLSPIIIYATLTVPRV
                     MLLEAFLSFLGLGVQPPMSSWGLLIREGVETMEEYPWLLFFPGILFTSTLFALNALGD
                     GLRDALDPRISGD"
     gene            12093..13094
                     /locus_tag="PKN92_04205"
     CDS             12093..13094
                     /locus_tag="PKN92_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48028.1"
                     /translation="METASPLLKVNDLRTWFHTRDGIIRAVDGVSFSIDQGETVGIVG
                     ESGCGKSALFYSLLGLLPMPPARIESGSAFFRGEDLLRASPERQRALRGRQIAMIFQD
                     PMTALNPYMKIGAQIIEPLLVHRLCTPREAHDRALAMMHAVGIQDGEQRMAMYPHEFS
                     GGMRQRVVIAMALITRPELVIADEPTTALDVTVQAQVLALLTDLQKQMGMSVALITHN
                     LGIVSQLCSRVIVLYGGRIMETAPAKQLFEKPLHPYTAALLASLPRSHRAGEALAAIP
                     GQPPEKGAPEAGCPFAPRCRYTQEACYKSDCPLLLCSPGRQTACLRVNAGELQELMP"
     gene            13091..14098
                     /locus_tag="PKN92_04210"
     CDS             13091..14098
                     /locus_tag="PKN92_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48029.1"
                     /translation="MTGTGDSRHLLQIEDISLRFPVRVRHGWKRQQGRLEVHALEKVS
                     LHLHAGEVLGIVGESGCGKSTLARSIVRLETPSSGQILFRGMDIHRMGTAALKRLRPE
                     IQMIFQDPYASLNPRMTVYDTLAEVLRIHRKLRGKALDLELAELLQKVGLAQRFVRKY
                     PHEFSGGQRQRIAIARALAPRPSLLIADEPVSALDVSIQAQIINLLADLQRDQQLAML
                     FISHDLGVVRHIAHRVAVMYLGRVVESAPRDTLFRTPRHPYTQLLLAAMPDLDKEKTA
                     DTAPVSVQGEPPSPFNPPAGCVFHPRCPIARPQCSQEAPPLLEVAPEHHAACPYGTSG
                     Q"
     gene            14253..16535
                     /locus_tag="PKN92_04215"
     CDS             14253..16535
                     /locus_tag="PKN92_04215"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNZ48030.1"
                     /translation="MTASDNNPYGTLLLFAAAALFILLCPLFWISVQAPGGGAVSPEG
                     ENAALYRQIFPSMLYGIESLRTGKLPLWNNRILCGTPYFADGRHGLLQPLTLPFLFLD
                     FPRALALHAFIALSASGFFFVLFARSLGLRFIPALTGALIAVCSGTFASAASNPLTLQ
                     TVMWFPLLLWMLREHMRKPRRDTLVLGALSLALLLLGGALMPAFVLALTALFYGALCA
                     YMGIDAEESGSTKNSRLQRILALIAMITLALLLFSIQGLPMLHRSLQLETPARLLSGF
                     RLAAALPSGIKGLWTQLFDLQTQLLPAPGRIGVLALLLLPPAFFHRLQRKERFFFGLA
                     LPALIILALFTRDAGWVQALYFPVSFCLAVLAALGMDRLFTVRRNPMTPRLWVPAAAS
                     CLLFIILFPIVPDSARGRMIPFLPALVFFLAIRRNWAATAAGIGILIFLYVDLASSFV
                     NRQDHPFFQAVPELRLHARTEKALRESALDGRALLLAAPDNRSFHPNSSIAAHLYGAL
                     GESAVLSRSQQKWLEQWNKTMAENNGVPSTAMMQLMSVRAVAADSSFSASENAPSGLN
                     LQQHSRDGGITVFNSPPGLSRAYWTPRWRLATDDRAALEGVCAPDFDPSRECLVTTAK
                     KTIHHLARIVAESTEESRDYEQPRIVLSEKRPEAVSIQLDTPGPGILTFSECFDEGWH
                     ATDNGVPAPLLRVNGLFMGIALDKGNHSIIFSYRPPLMLAGSFISALTALFCVILLLA
                     TYVTSAGKSRSNTCPANTSH"
     gene            16559..17716
                     /locus_tag="PKN92_04220"
     CDS             16559..17716
                     /locus_tag="PKN92_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ48031.1"
                     /translation="MDADFTAVAALVLAGGSGERFWPLSRPHRPKQLLDLDGSGKCML
                     RQAVDHLNPLIPPNQLYISTNRLLAPTVRKLLKDIPASHIYAEPCTKNTGGALIYTLA
                     RMLAAKGEEKLSAMTLALVTADQKIERRFCFHRMLREAVATAQAEDALVLCGIMPSRP
                     EKGFGYIETTVSTPFCARGKKMVAWPVKAFHEKPDAARARRFISTGRHFWNSGMLFCR
                     FSVLMNQLQKARPDMAALVYQLATLIREKRLEEVDALFATLEAISIDHALLEHADRIL
                     MVRGDFSWRDMGTWQSVAEERTPDDMGNVTAGNAAIMDCRDSIIWNAATDKGISVAAL
                     GLSDIVLVVTEDAVLVMHKEHAHDLKKLLAEMASKKKNVRQPKQKKDGDAH"
     gene            complement(17710..18675)
                     /locus_tag="PKN92_04225"
     CDS             complement(17710..18675)
                     /locus_tag="PKN92_04225"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNZ48032.1"
                     /translation="MQENESAPQAEEQHRSFRYWADKLRTAVLCLIAGITLYALYSLR
                     GSDWGAVYAFWHARRFLLAQVFLLGCCDIFLDGLIWRQVLRHQGVTPGKVQSIFLYFS
                     AHAALLMPAQLGRAFRSAETARRCYVSAATTFSAEVLYLGFTAISASALFVSAFMFFQ
                     ISATAFFLPLLLIPAGLAAAQVIRPLLIRLKLQIGFVSFLQPALAGLVFLSMLNWLIN
                     ALNLYLVFRELIPDLSYMHALMISTSTLFVGAISGIPGGFGVAESYIGALLYWLKAPP
                     VHLVPAVLAFRILSVWIWVPVGWLTLGLQSVAFRKKSTEETEASQ"
     gene            complement(18742..19998)
                     /locus_tag="PKN92_04230"
     CDS             complement(18742..19998)
                     /locus_tag="PKN92_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48033.1"
                     /translation="MKKRQDCYVFYVLSVLLALGASVMGDAFSCDRGAINVDPEHPAW
                     LLYGDNAPFYMCGPGDPEGFLYRGLRKADGTREGDQQALIRKMAGTGANCIYMIMVRS
                     HGGDGTADENPFVNGDPGQGLNTAILDQWDQWFSEMNRQGIVIFLFFYDDSAAPFHRD
                     LTDGALDPREEQFIDTVVARFHRHPYLIWCVAEEYIEAVSPARAAKIAERIKAQDPLR
                     HPVAVHQNHGTVFDFTDNIHVDQFAMQFNTASSDSLHEAAVSAWQNTGGRKNINLSEF
                     QPRPTGLALQKTIWSIAFGGAYSMILQMDIDKTKEEDLKICGGLVRFMESVRFNETAP
                     SDALARGDSRYVLARPGQLYLIYSETGRYPGVDLQAGCYTLQWFDISSGRWFHGGRKT
                     LSGGEQYFKKPASVGAAAVLYLVSCL"
     gene            20305..20976
                     /locus_tag="PKN92_04235"
     CDS             20305..20976
                     /locus_tag="PKN92_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48034.1"
                     /translation="MKLNAPVISRLALLIVCAVALSGCHFTIQRETEIILTENVTVSG
                     ANRINNTNGTLTENDLYEEVLLQGWKNGLVTFPLGTNCDFTKNILELAEKVKDPVQFL
                     DSPFAPLLKIFFVQGKLDGLVLTKIALEPSSGNFDTITRCSLRAFDETQGTINSYSAA
                     GPFQGKPVLTPDRNIDIYRVIRDAGVECLTSEMTMQGSRPQNDLIFTAKATAVYEVKL
                     RFFSF"
     gene            21424..22845
                     /locus_tag="PKN92_04240"
     CDS             21424..22845
                     /locus_tag="PKN92_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48035.1"
                     /translation="MLTKKTALILGAILVVMPLSIATAGTYSGGSGTADNPYLLSRAA
                     DWLELSETQSDWGSDFRLTRSIDFSGVTVKPVGRDFDPANPDYPHIYFSGSLDGGGYR
                     LKNIVLNYPDENDVALFFSMQRASVNNLIIENMEIKAKRQAASLAVYFDESAAKDVKI
                     ISRISAEGDAGGLAVDCLNSKFMRCWIGTDIQCTDDNSRAGGIAALEQGEEISFSESI
                     GTIVAKERAGGFFATALDLAIRNSTSFATTQSAIAGGFVGYADKLTVTKSSALIDVRG
                     YNYGGGLAGYAQNSNFFNTFTRGSVSGNNAAGGLVGFCDHNVSFKESFSSVAASSVLG
                     DSGGLAGVLRNSTLTNCYARGQITGVGFVGGIAGSAGQSTLSNCYAAGGLTLFGANRN
                     AGGLLGYADNTSLDFCYWDTEVSGTAYSAGGDGRTTNAMTYPHAVNTYTKWRFGIFWT
                     ADTDSTVNDGTPGCAIPLLLHSK"
     gene            23049..24554
                     /locus_tag="PKN92_04245"
     CDS             23049..24554
                     /locus_tag="PKN92_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48036.1"
                     /translation="MRSVGWDYDPDNEFHPLPSFTGSLNGNYFHLRNVNLNRPDMNDV
                     ALFYSLKGARIQNLFIDNISVDARTYAGALAVYISSSTVSRCLMSGSVQSLDCAGGMA
                     TLISGDSQIQSCILQTNVRVRNELNPVGGSAGGITCFTQDTAFLKCSSAGTIVGKTEA
                     GGLTAHSLASSFSECYAHVTVQASVAGGLAASVKDGSFTDCYARGIASGKTYAAGLIA
                     KTDGMVLNNTDNAEINRCFAAVRTRVDGEGEIAAGLIASSTNTNVTASYWDSERSGNN
                     SSVGGERRTTADMSFPYAANSFVGWDFNAVWASDPDYSKNSGYPWLRNLPPTAVILLG
                     DKYSGGEGTAAKPYQIASVADWQQLCRSQEDWTKHFEIIRDLDFTGVIAVPLGRNYNR
                     IHPDHPVPFFQGTLEGNHKRLINLSMPQAEEEDVALFYHLKNAVIRNLNIEQVHIVSG
                     ERAAALAVYLEDSTVINCFVSGTISGDAYAGGDRRQNNVPGQPGAEMPGGC"
     gene            24511..25563
                     /locus_tag="PKN92_04250"
     CDS             24511..25563
                     /locus_tag="PKN92_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48037.1"
                     /translation="MSQGSLVQRCRADVEIHTTADYATAGGIAALLLDGTVEQCSSAG
                     TIQGKSWSGGLLGNIFSGNIARCTSVAHVTGRSAGGMAGLIDRGTCSTSYAHGRVQGE
                     VAAGFAAETVSSVLNDCYARGQTRGVHCAAGFIARLNNRLNDKADNAQINRCYAAVRL
                     KLTSDNGIAGGLIGENGGAEVITSYWDKEYSGTLISAGGAGRTTTEMTHPHAANTYIN
                     WDLNGIWAADENGNMNAGYPWLRNLPRPPQDDENDIEGEEPDVEGEEPGIEGEEPGIE
                     GEEPDVEGEEPGIEGEEPEVEGEVSINPEKKGCAGCGCASKDLRTSVKDLLGDWLLVA
                     LCLSVLFMSTLLRRKV"
     gene            25746..26363
                     /locus_tag="PKN92_04255"
     CDS             25746..26363
                     /locus_tag="PKN92_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48038.1"
                     /translation="MKKTMFRMLFLLSLTTAMALFSGCHYDLDQTASFDILSKPVLLD
                     TALLQTLPGGEFKLELEENCEIPAQVRALVKKLQDQITNQEIDLGAYGFLPFIKPKIE
                     VRALHINEVDLNATKNTFTGINALSFEIKDSSQGKSIRLQSQEITPQQIPLRPVENMD
                     WLAFLMSPDMECMETSLSFTGTPPEKPVSFTARAHVTVKVRILLF"
     gene            26549..26734
                     /gene="rpsU"
                     /locus_tag="PKN92_04260"
     CDS             26549..26734
                     /gene="rpsU"
                     /locus_tag="PKN92_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009564692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNZ48039.1"
                     /translation="MTKVRVKTDEPFEKALRRFKKKCNKEGLMQRLKEVTYYEKPSET
                     RRRRLAKAVARAVMEEL"
     gene            complement(26808..27101)
                     /locus_tag="PKN92_04265"
     CDS             complement(26808..27101)
                     /locus_tag="PKN92_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48040.1"
                     /translation="MTKDVGPLEVWAGNPARKVAHRVKGLPESMLARYEELLKRFGLR
                     ENRFGYHEEACRAGEAAMDGINRAAIERDRLAALFNPPVEEEQKQAEAEVGEV"
BASE COUNT         6375 a         7650 c         6933 g         6406 t
ORIGIN      
        1 ctgctcggcg gtctgctgtc tctggatctg atcacactgt ccgacggaaa ataccagaac
       61 acagaagcct cttccgcatg ccttgtcacc ggaggagcag cctaccaggg caatctgatg
      121 cagcatacca tgaacgggcg tgacgcctgg gcatctctga ctgcctgcat ccagtcgggt
      181 acctgccaga tcgatgtgca gcgtcgtagc ggagaggcgc tgaagagctt tattctgggc
      241 atgcgcaata tcgcccaaat gagcgcccgg gaatgcctgg ctgctgttga tctgtccgga
      301 ttccggcata tgctggatct tgcaggcggt cccggaactt acggcattac gtttcagcag
      361 cagtttccgg agctgcatgt cactcttttt gacaggcctg aagtggtagc gattgcccgg
      421 gaacaggtgt cggaggcggg gctgtcttcg cgcttttcct ttatcggcgg tgactgcatc
      481 tgcgatgacc tcggcaacgg ttacgatctg gtgttcctct ccaatatcat acacagtttc
      541 ggcacggaag agaacgccgg gttgatgcgc cgcgcttacg atgcgcttgt accgggcggc
      601 acgctgatca tcaaagattt tattctggat aacgaccggc agggccccgc ctacgggctt
      661 atgtttgccc tgcagatgct tgtgcatact acggcgggaa atacttacag ctttgaagag
      721 attcaacgat ggactgatgc cgcaggcttc cggcagggtg aaagcatttc gctgacacca
      781 cagacccgat tgtggattgc cagaaaatga cctgtttccg gcgcctgtaa aggcctgttc
      841 atcaaaccga caatatgtgt taaaggattt gttatgctct atttcaaaac ggctcagtac
      901 attccgggga aaggcgatgc ctggacctat tacgaatgcg atgaaaacca gaccatcgtc
      961 cgtcagctca cccatattcc tgaaacgggt gacatcgacc gaatccccga ccctatcgta
     1021 aagaaactgt atcgaccgga acgactgctt ccggcggaag cacaggaatt ccaggaactg
     1081 tggggcgaag gctgaagtat catgtcttta atcagcccct accagtctct tgccgaccgg
     1141 cagtttaaag ggaatcttca tgcccacagc acactgagcg acggtatttt ttcccctcag
     1201 gaagtcatag atctgtatgc gaacaactgc tatgattttt tgatgctcag cgaccatgat
     1261 attctcagca atccggcaga ctggaaggat cgggggatga tccttatccc gggcaatgaa
     1321 attaccgcaa acgggtgcca tctgcttcac gtcaatgcac atgagcttat tgagccgaat
     1381 cccgaccggc agcaagtcct gaacgcaata cagcgttccg gcggctttgc catccttaac
     1441 caccccaact gggggttcaa tttctgccac tggccgcagg cggaactgga acggcttcgc
     1501 ggttatgcag gcattgaaat ctacaatggc gtgaccgagc gtgccgaggg aaatgcacag
     1561 gcaaccgacc ggtgggatat gctcctgtct cttggcagga aggtatgggg ctacggcaac
     1621 gatgactgtc acatttcagc ggatatggcg atcgtatgga atatggtatt tgcggaagag
     1681 cgctccgcag aagcgctgct tcgcgccctt gaagcagggc aattctatgt ctcgacaggt
     1741 gtccggctgg agcggatctc ccagtgcggc aaccgggtgc acatcagctc tccggacaca
     1801 gtctcctacc gcctgatcgg agactatggc accattttcg ccacagcgga gggaccggaa
     1861 cttgattttg aagccgaccc gacgctgccc tttacttact ttcgcataga gtgtttcggc
     1921 aaggggcatt cccgggcctg gacgcagcct ttcctgctgc aggcacaggc gtaagtacta
     1981 cggtttcctg cacctgcagc gcatatcaga catcagccag ggacacctag gcgagcgctt
     2041 ccttgcagaa ctgaacgcag cgggtcactt ctttcactgc atcggatccc tccggaatat
     2101 cgtattccag ttctatatcg gcagggaagg ttaacccttc ttgcttaagg aactgcaata
     2161 cttccgccac ctgcgtatcc ccttcgccaa aaggcatatt cggtccgtct gaaaagagtt
     2221 ttttcttttt ccggtctttc acatgaagcg ataaaatgcg gtcgcggtgc ttgtcaatca
     2281 tggggatggc aggctcgcct gttcccgcca catagtgccc gatatccaga ttgatcccga
     2341 gataacgtcc ataagaaagc atatcgccgg tgtaactgtc cggagtgatc tgggtatggt
     2401 tgtggaagcc gatcataatt tcatgcctgt ccgccaccgg tccgagccgt tcagcgagcc
     2461 cggacttgtc cggcatctct cttgtaatac cccgggcacc cagcgctttg gcaacagcga
     2521 aatagtagcc catttcggca tcgctcatct tctcgtcgcc gatatgtccg aatttcacga
     2581 tatggatgtt gactccggct tcattataga gcttacgcag cgcctcgaaa ccgtccatgg
     2641 gaccgcccac acccgtatca ggcgtgtgcc gtttcgcaaa atcctctgct gcatcgccca
     2701 tcagttcaac ggagctgatt ccgcagcggg taatatagcc aagcagttcc tcggcactgc
     2761 agggcagaga acgaaaactg taggtaatga cgccgatctg tacgccattg aatttggaat
     2821 cgggtttttc ggcagaagcc gggagggcat gcagactgag ggcggcagcc gacaatgcag
     2881 ccgcaccacc tataaactgg cggcgggaca gtgaaaaaga cgaatgatcc atgtgaacct
     2941 tctttctgtt accgggtagt tctttttatt tttcgtacgg caggacttat gcccaaccgt
     3001 caaaacactt cagcagatcc tgaatggcat tctccgggcc gtccgtgtag ccgcattcca
     3061 ctgcaagata accgctgtac cctgtttctt taatggcttt gaaaacattg gggtaattga
     3121 tctcgcctgt tccgggctgc ttgcgaccgg gattatcacc aatatggata tgcccgattc
     3181 cctcgatatt gtctttaata ttgggaatca gattgccttc agaaatctgc tggtgatata
     3241 tatcgtacag ctgtttgatg ttcgggctgc ccacagcctg cacaatcatc tgcgcctggt
     3301 cggttgttgt cagccagaag ttctgatgat cacgtagctg attgagtgtt tccagcacca
     3361 tggtgatgcc attgtcttca agcattcctt tgacacggcg cagaaattta atcacgttct
     3421 gtgccgcctt gtcataggta atcgtccatt ttgaactggt ttcacctgtc agtccgacga
     3481 tacacttgca gttcagacct ttcgcctgtt caatggcggc ggcacactgc tcttccaggc
     3541 ggtcatgctg ggaaagatcc agcatttccc cctgggtaat tctgcctgcg cccatgatac
     3601 agctccaggc aacaccggtt tcctgggaag cggcggcata ggcatcataa ttttttttat
     3661 cctcgcctgc gccaagcaat tccaccgccg gaaaacccca ttcagcggca agcctgaaca
     3721 catctatggg gcggttccct ttgggaagac agtcgctgag ccatccgtaa ctgcaggata
     3781 ttttaagcgg cagtttggaa aaaggctttc cttcgggaac ggctgcctga gcactgctcc
     3841 cgcccagagc ggaccacata cccgcaccgg ctgcagcggc ggttaaaaaa cttcttcttt
     3901 tcatttccat gcgttctctc ctttagcaag tgtttctctg attgtcacag tgactgcctg
     3961 atgctttttc acgataactg ccttgcaggc tcaggcttat ttgatctttt attcactcat
     4021 tgcgatgtac cacaacaata ttttgccaaa aacatacccc tattcagtct tttttatcaa
     4081 gcggtgtcag atacacattt ctgaagcggt gctttcctcc gcccggcacc tcggcctgaa
     4141 aggcgacgta gcccttctca gggccttcca gcccctccgc ttcccaagcc ggctttccat
     4201 tgatttccag tgccgctttt gttccctgca cggtcagctt aaactgattc cagtcccgct
     4261 ccttaatcag gcctgtgctt gaagcacctt tcaattcgct cacattccct tcaagccctt
     4321 ttctcagatt cgcctgatac cggggcggcc agggtgcgcc ttcaggaatt tctgtataac
     4381 ggaaatagaa accgctgtcc cagtcatctt cagccagcgc tttccattca aattcccaga
     4441 taaaatctcc gtattgctcc gcggactgaa ccatgccatt gccgctttca atgagcaggt
     4501 cttccccgtc cgctttcgct tcgcattggt acagggtcca tccgtccaga tttttcccat
     4561 taaaaagaag aatgcgcgaa cagtcctctg aggcaaaaac gcccgtaccc atgaagacca
     4621 gacttactgc aataagaaag aaaagcacgt atttcatcct gtttcctttt tgttggcgtt
     4681 tattttattc tgcttccggc tgacatgccg ggaacagttc gggatgatga tccagtatcc
     4741 ggctccaggc ttcagatgcg gcaccgttac cccaggtgta aatcgtaaaa ttgcccatac
     4801 cttctccgtc cggagtggta acaccgccgt gcccgtaatt ttccatcata aagccgtaat
     4861 cttcagtgcc gaaaaaagtc catacttttt cccacagttt ctttacagcc ggattctcgg
     4921 ggcagtacat catgacaaaa ccggacttca ttgcggcaat gccccgctct atgtaccgct
     4981 gctcgcccaa cactttcccg tattcaatga acagctcggc aaacagcgtc tggcgcgagt
     5041 cattccattc gccgtccata ttcatcacgc cccagccacc cagggcggga acataaataa
     5101 acggcggctg ccacacctgc tgtaccatgg aaagttcatc cagcgtacgc ctgccccagg
     5161 aaaggtactc ctcacgcccg gaaatgcgcc acgcttccag cagcgcttcc gatgtccaga
     5221 acatggataa ggtgttctgc ttgaacatgg cgtttcgttc aaaacgcctg cccagatgat
     5281 cctgcccgag acgacagcac gaccagtagg tttcataatc ttcccattgt ccggcaggaa
     5341 taatttctct gatgacggca tccagagcac gcagcgctgc cgtctcatat tttttctcgc
     5401 cggtcatctg cgccagcttc agcagaaaag tgacgctcat gctagtctgc ggtgactgtg
     5461 ccagcactgc agaaggcttc tgcgtttccg gatccagcca ggaagggaaa aatccgtctt
     5521 catcctgaag ctgcaggagg cgctcggcat atgtcgaaac atagggaaga atgcgctcat
     5581 ccggctcaat ctcttcatgc cagcgcagca gcaacagcgc tgtccagctc atgtccagca
     5641 cgttgtacca gcgatctgta ataccatgct cccagggcac ccggttgctg ttgacccaat
     5701 atccgacatc ccagccctta ggccgatgca ccgttacccc gtcaatcgtg acccgttcca
     5761 catccgaccg gtacactgtg gggaatacgc ctttatccag gggtgccgcc agcgtaaatt
     5821 cttcggtaag ccgggcacga cgcatcaagt cttcattgcc tgtgcgtctg ccgtagcgaa
     5881 ataccccgga agcggcgcgg agagacgaaa accacgcctg attccatatg gaagggaatt
     5941 cgcgctgatc cgcatgaccc ggataattgg gcgaatccgt aactgtcaca ataaacacgg
     6001 gggatccgac acgggttcca tgcagatcaa attcctgcca tacggcatgc gcccagcggt
     6061 caaatgccca gccataggtg tgctccacat aacgttccgg tgaaactgta gtcggctcac
     6121 cccgatcata aagcgctttg ccccagcgat cccagagaaa ggctgctacg gactgccacg
     6181 ggttaaaaat atcttctttc ccgtccagcc atgccagata aaaagaaaga tcgacggagc
     6241 ctgtgcccag tgtcatgccc ggtacttttt taaaaagaac atgctccggc acttccgtgc
     6301 ggctcatgcc caatccgaaa tatttttccg gtgcattgaa atccatatac cagggggcag
     6361 ctgtatcttt tccgcacata tccagatccg gcacaagggc aaggacgcct tcttcggaag
     6421 cggctatcac cgccggagac cggaatacat gctgcgcaat gaccagacct tcttccgggg
     6481 tcagatgcgg tgcccaccag aaattcgggt caaactgaag gtgaaaaggc ataaaaagat
     6541 cgttttgttc aaccgcttct tccagctccc agcgcaaatg cacacgcacc cacttccccc
     6601 attgatcaaa gcgcaccaca ggcacacggt catttacgga cggaatatcc gcccgtgcca
     6661 gttcccgatt cccggcatgg cacagaagta ccttcccttc aacccggaac tgcacggatg
     6721 cttcctgtgc ggcacatacc ccggaaagtc cgaataaaag caggacaagc aagcgagaca
     6781 taaaagagtc tttctcttct gattttaagg aaaaccaaga tcttcattat actgttttgc
     6841 ccgggaaaag cataaatccg gcttctcttt tgttatgtct gtacaggaga acagaaggca
     6901 ctccgactgt tccgtgcacc ggctgaaacg gagcgcccga atcagaaaag agaaagactt
     6961 cacaatgcgt ccgcgctttt cccttagctc tcggcactct atgctgtaca tgctgatcac
     7021 tctggtttta atcaccgccc cggatattca cgccatgaac cctcttaccc cggaagaaga
     7081 atatgtcatt gtgcacaaag ggaccgaacg ccctttcagc ggaaagtaca atcgccattt
     7141 tgaaaaaggc gtgtaccact gcagacgatg cagcgctccg ctcttcaatt cttcctccaa
     7201 atttgaggcg gggtgcggct ggcccagttt tgacgaagcg ctgcccggtg ccgtgcaatg
     7261 gattcccgac gccgacggca ggcgtacgga aattctctgc gcccgatgcg gcgcacatct
     7321 cggtcatgtt ttccagggtg aagggttcac tccgaaaaac acgcgccatt gcgtcaattc
     7381 cgtttctctg gactttcaga aaatcccggc gacttcacag gagcaccggg ctgtctttgc
     7441 cgggggctgt ttctggggtg tggagtatta ttttcagagc gctgaaggcg tgctgtccac
     7501 ccgggtcggt tatacaggcg ggcataccga atttccggat tatgagcagg tctgccgggg
     7561 cactacagga catgttgaag ccatagagat cctgtatgat cctgcccgaa ccgattttga
     7621 gaaactcgcc cggctctttt ttgagataca tgatccgacc cagaccaatg gacagggtcc
     7681 ggatcaggga gagcagtacc gttccgtgat cttttatcag aatgaggagc agcgtacaat
     7741 cgcagaaaag ctgatcgcgc acctcagggc aaagggcttt gctgtagcga ccgctttaga
     7801 acccgcatcc gcattctggc ccgccgaaga ctatcatcag cgctactacg aacgccgccg
     7861 gggcacaccc tactgccata tacgtacgcc ccgattcgaa tagtccgccc cgccctttat
     7921 ttgacacgca gcgacattgt ctgtagacta ctgggcatta caggacagga atggggtgca
     7981 aacctaccat tgaggccaag catgagcgca agtcgcaaac agttgaattt agacggaaaa
     8041 tggttttttg ccgcagaccc ttcagactgc gggcgtaaag aacagtggtt cgaacgtttt
     8101 ccagacgata ccgacgagat ctatcctcct tttttgtcag ggcaattgcc gggagacggt
     8161 ggaatcggat ggtatcagca tagctttgct gcaggaccca gttggagtca gagcagcact
     8221 ttcctccatt tcgagcgcgc ctcctatttc accgaaatct ggctgaacgg gcactatctt
     8281 ggatcccatg aaggggcgtg gcttcctttc tctgtgccaa cagaggaaca cctgcatccg
     8341 gacagcaaca cgcttgtcct gcgcattgtc tttccgggac agcatgccgg cgatgccgat
     8401 ctgaaccctg cttctcttcc ttattatggt gaaacaggca gcacaggagg gctgctcggg
     8461 gctgtgtctc tgcttgcgta tccgtctgca catattgaca gtatttttgt gcagcccgat
     8521 ttaaggcggg ctcggcttgc tgttgacgtg tcctgcaccg gcggcactgc taaaacggaa
     8581 attgtactca gggttaagga taccgactat tccgttaccg gcaagccggg agcactgctc
     8641 ctgcccatgc ccgattttca ggcgtggact ccggaaaaac cgcatctcta ccggcttgag
     8701 gttctgctga aagatggcag gaaaacagtg gatgccgaag ccctttcttt cggtatgcgc
     8761 gaatttaccc tgaaagacaa ccgctttcat ctgaaccagc gcccgctctt tatcaaagcg
     8821 cgggcatgct ttgccgcacc atggctctct cttgaaaaaa cagcacgcct tcagactttc
     8881 aggcaattca tcgctgaaag ccgtgatgcc ggattcaata tgatcacgct gacagggcac
     8941 cctgtgcccg aagatttcct ctgcgttgcc gatgaggaag gcatgcttct ttctgtggaa
     9001 tcctcgctgc gtcccatgac tgcatcggat cggctggaac agcgctgtga agatgcggtc
     9061 accgacatga tcagaaacgg caggaaccac gcctcgctgg tactgtggcg gctgtttaat
     9121 gaagaacgga cccgtgcggc agaaaccgga aatgccgccc gtgctctaag cgaccggctt
     9181 ttcacactgg caagagaact ggatccttcc cgcctgattt ttgcgggcgg cgcacatgca
     9241 gacagttccg atgcaaaact gctccgcccg taccgaagca ctccggaacc ttacgacgat
     9301 ctgtttttct gtcccaccga tcccgtaccg gaaaatcgga cacggtacct gcaatgctgc
     9361 ggtgctgccg gtaaactcgc catcatcagc gggatgacct catttcagac cgccgagcat
     9421 tcggaggaag acgacacgct tcacgctgat tttgatcagc gcgggttgaa ccggatcttc
     9481 ggcagccttg ctgctttcaa tgaccttaca gacgaatcgg caaaaacatt tgtggctgca
     9541 caaatccagg ctatgcgcgc caacagtaaa attgcaggct attttcacgc tttccctgaa
     9601 agcagcggct gcacctccga aaaaccgaat gccctggaaa tagtggcggc ggcgcaggcg
     9661 cctcttctgc cgctgatcag tctggaaaaa agcaacctgc acccccgtga agccaccggc
     9721 gtcaccatcc agatgatcgg cgatcccggg cagggtggtt ccgcagatct gtcattgcag
     9781 gtgatcgggc ctaccggtca ggtgctgtgg aagaaaaagc ggcataccaa actgccgcgc
     9841 cacggacaac tgctctggtc tggaagtatc ggcggctccg gcgcactcgg gcttcaccgg
     9901 tttgttgcaa gcctcatgca gggaacccgg gtgctgggca gaggcgaaga ctcctttttt
     9961 gtctttgaac aggcagcacc ctcacccgtg gaagtccatc ttctggaccc tgaaaaaatc
    10021 tatcttgacc gtgtagcagc tctggcaaag acaggtaatc tcctggcacc acttcacatc
    10081 ataccgcctc tcagcaatac cgttcgcgcc tacccggaca acgacctgat gcagattctc
    10141 gcacaggttg aagaaggtgc cgttgcgatt gtcttctcgc cacccgaaga ttggaacgat
    10201 ctgttttcca tactggatgc aaaaataagc gccacgcccc gtcccgcttc atgcagttgg
    10261 gcatccgcct tccactacgt caaactgcat cccgttttcg aaaaacttcc gtcccgatgc
    10321 ctcatgcgtg aggtctataa aaatgttgct tcggcactaa actttgaaga acagggagaa
    10381 gaagatatct ccggaagcct ttgccgcact gcggatgcac ttaccggaga aagcacggcg
    10441 cggtggggaa ataacattct ggttcagcgt tacggcacag gccgtattgt catgacctgc
    10501 atgcctgttc tggagcatct gggaaaagat cccgtggcag accgtctttt tgtaaatctg
    10561 ctcaatcatt ttgagcggcg cagtatcccc tccgcaagcc ccgtaccgcc caatcagcgt
    10621 gccgttgaat ggctgcgtga ggaaaagaag acacagctcc gccgctggct gattctcggc
    10681 ggcatcgatc tcagttcgga tgaaaacaga aaaaaaagtc ttcactccat cgaaatggaa
    10741 agaaattttg aggctgctgt ggaaggctgg taccgaccgg aaaaatggcg cacctggttc
    10801 acccgggcag atgcggatca cgaagtaccg cttaccgatg cgttggatcc gggcggcttt
    10861 ttcttctctc actatgagcg ctctgccagc tatgcctaca ccgaaattac gtgcgaacgc
    10921 cgactcagca gttatttgcg tgtgcgcttc cattcgccca tccgcatatg ggtgaaccag
    10981 gtccccgtat ttgaaagcga agggccgcct gacggagaaa actattgcat cgaaagcgtg
    11041 aatgcaactt taaaacatgg aaaaaacacg ctgcttgtca aatgttccaa agaacgcggg
    11101 aaagcgctct ttgcgctgga ttttgtatcg gacgaccact tgccctcggc gctgaaatgg
    11161 tggaaatgaa tctccggcgc ggcacttcac tgtatccgct gccacaggat cacccggatc
    11221 atggatgaac cttctcttga aaaaggagtc tccctctggg aggacgcctg gcgtcgcctt
    11281 cgaaaaaaca agttggcgct ggcagggctg gtcatgttga tggtggtcct gctgctgtct
    11341 gttgcagggc cctggtgcag cccttacggc tatgaaacgc aggatacggc tctgggcgcc
    11401 gcggcaccgt ctttacgcca ttggctcggc acagatatgc tcggcagaga tctgctgacc
    11461 cggcttctgc acggcggcag agtgtctctc ggcatcgggc tgtccgccac actggtttcc
    11521 cttttgatcg gagttctcta cggagccttg tccggatatg caggcggcta tacggaccgt
    11581 gtgatgatgc gtatcgtcga tattctttac gcccttcctt ttaccatttt cgtcattatc
    11641 ctgatggtgt ttttcgggcg gaatttcatt ttccttttta tcgctatcgg agccgttgaa
    11701 tggctcacaa cagcacgtat tgcccgcggt cagaccctct ctctgcgaca tcgtgaattt
    11761 gtggaagctg ctgtgattct cgggtacagc cgcatgcgca ttctgctgcg acacatcatc
    11821 cccaatatgc tgagtcctat tatcatctat gccacgctca ctgtgccgcg ggtcatgctt
    11881 cttgaagcgt ttttaagttt tctcggcctg ggcgtacagc cccccatgag ttcctggggg
    11941 cttctgatcc gggaaggagt ggaaaccatg gaggaatacc cctggctgct tttctttccc
    12001 ggcatactgt ttacgtccac cctcttcgct ctcaacgcac ttggcgatgg actccgtgat
    12061 gccctcgatc cccgtatttc cggagactga ctatggaaac agcgtcgccc ctcctgaaag
    12121 taaatgacct gcgcacgtgg tttcacacgc gcgacggtat tatccgtgcc gtggacgggg
    12181 tcagtttttc tattgatcag ggagaaactg tgggcattgt cggtgaatcc ggctgcggaa
    12241 aatccgcgct tttttactcc ctgctcgggc ttctgcccat gccgcctgcc cggatagaat
    12301 ccggatccgc ttttttccgc ggagaagacc tgctccgtgc atctcccgaa agacaacgtg
    12361 ctttacgggg gcggcagatc gccatgattt ttcaggatcc catgacagcg ctcaatccat
    12421 acatgaaaat tggggcgcag attattgagc cgctgctggt tcacagactc tgcacgcccc
    12481 gggaggcgca tgaccgggca ctggcaatga tgcacgccgt gggcattcag gacggggaac
    12541 agcgtatggc catgtacccc catgagtttt caggaggcat gcgccagcgg gttgtaattg
    12601 ccatggcact gattacccga ccggagctgg tgattgctga cgaacctact acagcactgg
    12661 atgtgactgt gcaggcacag gtacttgccc tcctcaccga cctgcagaaa cagatgggca
    12721 tgtcagtcgc gctcatcacc cacaatctgg ggatcgtatc gcagctgtgc agccgggtaa
    12781 tcgtgctgta cggcgggagg attatggaaa ccgcgccggc aaaacagctc ttcgaaaaac
    12841 cgctccatcc ttatactgcc gctcttcttg catcgctgcc ccgatcacat cgggcaggtg
    12901 aagcgctggc ggctatcccg ggccagccgc ctgaaaaagg tgcgccagaa gcagggtgcc
    12961 ctttcgctcc ccgatgccgc tacacacaag aagcctgtta caaatcggac tgcccgctcc
    13021 ttctctgcag tccgggacgg cagactgcct gcctgcgggt aaatgccgga gagctgcagg
    13081 agttgatgcc atgaccggta ccggagacag cagacacctt cttcagattg aggacatcag
    13141 cctgcgcttt cctgtacgag tccgccacgg atggaagaga cagcagggca ggctggaagt
    13201 acatgcgctg gaaaaggtgt cgctccatct tcatgccggt gaagttcttg gaattgtcgg
    13261 cgaatcgggc tgcggaaaat caacactcgc ccgaagtatc gttcgcctcg aaacgccttc
    13321 ctccggacag atcttattcc gcggaatgga tattcaccgg atgggtacag cagcgctgaa
    13381 gcggctccgt cctgaaatac agatgatttt tcaggatccc tatgcttctc tcaatccccg
    13441 gatgaccgtc tatgacacgc tggcggaagt gctgcggatt caccggaaac tccggggaaa
    13501 ggcactcgat ctggaactcg ccgaactgct gcagaaagtc gggctggcgc agcgtttcgt
    13561 ccgcaagtac ccccatgaat tctcaggcgg gcagcggcag cgtattgcca ttgcccgggc
    13621 actggcgcct cgtccgtccc tgctcattgc tgatgagcct gtgtccgcac tggatgtatc
    13681 cattcaggcg cagatcatca acctgcttgc agatctgcag agggatcagc agctcgccat
    13741 gctctttatt tcccatgacc tcggtgttgt ccgtcatata gcgcaccgtg tggcggtcat
    13801 gtatctgggc cgtgtagtgg aatcggcacc gagagatacg ctctttcgta caccccggca
    13861 tccgtataca caactgctgc tggctgccat gccagatctc gataaagaga agacagcgga
    13921 tacagccccg gtttctgtgc aaggcgagcc accttctcct ttcaatccgc ccgcaggctg
    13981 tgtgtttcat ccgcgctgcc ctatagcacg cccgcaatgc agccaggaag caccgccgct
    14041 gctggaagtt gctccggaac atcatgccgc ctgtccctac ggcacttccg ggcaatagaa
    14101 tatttcatta tcccgtaatg catcatggaa cgcttcttct ggaaaaagaa tagccctgaa
    14161 gagatcatcg gaaactccgg atttcctctc ccctttcccg ctgcagtatc atacatataa
    14221 gagaaaacat tttttacggt tatggagata cagtgactgc atcagacaac aatccttatg
    14281 gcacgctgct cctttttgcg gcagccgctc ttttcatcct gctgtgcccg ctgttctgga
    14341 tcagcgttca ggcaccgggc ggcggtgctg tatcgccgga aggcgaaaat gccgctttat
    14401 accgtcagat ctttccgtcc atgctgtatg gcatagaaag cctccgcacg ggaaaattac
    14461 ctctgtggaa taaccgtatt ctctgcggga caccctattt tgcggacgga cggcatggtc
    14521 tgcttcagcc gctgacgctc ccctttcttt ttctggattt tccccgggct ctggcgcttc
    14581 atgcgtttat tgccctgtcc gcttcaggtt ttttcttcgt cctttttgcc cgatccctgg
    14641 gactgcgctt tattcctgcg cttaccgggg cgctcattgc cgtctgcagc ggaactttcg
    14701 cttccgctgc gtccaatcct ctcacgctgc aaaccgttat gtggttccct cttcttctgt
    14761 ggatgctgcg ggaacatatg cgaaaaccac ggcgtgacac actcgttctg ggcgcgctgt
    14821 cgcttgcgct cctgcttctg ggcggcgcat tgatgccagc ttttgttctt gctctgaccg
    14881 cactgtttta cggcgcgctc tgtgcctata tgggcatcga cgccgaagag tcaggctcga
    14941 caaaaaacag caggctgcag cgtattctcg cactcatcgc catgataacc ctcgccctgc
    15001 tgcttttcag catacagggg ctgccgatgc tgcacagaag tctgcagttg gaaacccctg
    15061 cccggctcct gagtggtttt cgtcttgccg ctgcgcttcc ctccggaatc aaagggctct
    15121 ggacgcagct cttcgatctg cagacacagc tgctgcctgc tcccgggcgg atcggtgttc
    15181 ttgcgctgct cctgctgcct cctgcttttt tccaccggct tcagcgaaag gaacgctttt
    15241 ttttcggtct cgccctgccc gcactgatta ttcttgctct ctttacacgg gacgcaggat
    15301 gggtgcaggc actgtatttt cccgtcagtt tctgtctggc ggtgctggcg gcactgggca
    15361 tggaccgcct ttttactgta cgtcgcaatc ccatgacgcc ccgactctgg gttcctgcag
    15421 ccgcatcctg tctccttttt attattcttt ttcccatcgt accggatagt gcacggggac
    15481 ggatgattcc ttttctgcct gcgctcgttt tttttcttgc gatccggcgc aactgggcag
    15541 ctaccgctgc gggtatcggc atactgatct tcctctatgt ggatctggcg tcttcttttg
    15601 taaaccgtca ggatcacccc ttttttcagg cggttccgga actgcggctt catgcccgga
    15661 ctgaaaaagc acttcgcgaa agcgctctgg atggcagagc actcctgctc gccgcacccg
    15721 acaaccgcag ttttcacccc aacagcagca ttgccgcaca cctctacggt gcgcttggtg
    15781 aaagtgctgt cctcagtcgc tcacagcaga aatggctgga gcagtggaac aaaacaatgg
    15841 cggaaaacaa cggagtccct tccactgcca tgatgcagtt gatgtctgtc cgtgctgtag
    15901 cggccgacag ttctttttcc gcttcggaaa acgcaccttc aggcttaaac cttcagcagc
    15961 acagccgtga cggcggcatc actgtcttta acagcccgcc ggggctgtcc cgggcatact
    16021 ggacgccccg ctggcgcctt gctacagacg atcgcgccgc actggaaggt gtctgcgcac
    16081 ccgactttga tccgtcccgg gaatgtctcg tgacaacggc aaagaaaacc attcatcatc
    16141 tggcacgcat tgtcgccgaa agtacggaag agagccgcga ctatgagcag ccgcgcatcg
    16201 tactgagcga aaagcgtccg gaagccgttt ccattcagct agacacgccg gggccgggca
    16261 ttctgacctt cagcgaatgt tttgacgaag gatggcatgc aacggacaat ggtgttcctg
    16321 cgccgctgct ccgtgtcaac ggtcttttta tggggatagc cctggataaa ggaaatcatt
    16381 ccatcatctt ttcctatcgc cccccgctta tgcttgcagg aagttttatc agcgcactga
    16441 cagcgctgtt ctgtgttatt cttcttctcg ccacctatgt cacttcagcc gggaaaagca
    16501 ggagcaacac ctgtccggca aacacaagcc attgacttga cagggagatc atttcagaat
    16561 ggatgccgat ttcactgctg ttgcagccct ggttcttgcc ggaggttcgg gcgagcgttt
    16621 ctggcccctg tcgagaccgc accggcccaa gcagctcctt gatctggacg gttccggaaa
    16681 atgtatgctc cggcaggctg tagatcatct gaacccgtta attccgccaa accagctcta
    16741 tatcagtacc aacagactgc tggcgccaac agtccggaaa ctgctgaaag acattccggc
    16801 gtcccacatc tatgcggagc cctgtacaaa gaatacaggc ggtgcattga tttatacgct
    16861 ggcacgcatg ctggcggcca aaggagagga aaaactttcc gccatgacgc tggcacttgt
    16921 gacagcggat cagaaaatcg agcgccgctt ctgcttccat cgcatgcttc gtgaggcagt
    16981 cgccactgca caggcggagg atgctctggt actttgcggc atcatgccct cacgcccgga
    17041 aaaagggttc ggctacatag agacaaccgt atccactcct ttctgtgccc gggggaaaaa
    17101 gatggttgcc tggccggtca aagcatttca tgaaaagccg gacgctgcac gggcacgccg
    17161 ttttatcagt acagggcggc atttctggaa cagcggcatg ctcttctgtc gtttttccgt
    17221 gctcatgaat cagctgcaaa aagcgcgccc tgatatggca gccctggttt atcagcttgc
    17281 gacactgatc agagaaaaaa gactggaaga agtggatgcg cttttcgcca cgctggaagc
    17341 catcagcatc gatcacgccc tgctggagca tgccgaccgc attttaatgg ttcggggcga
    17401 cttttcctgg cgggacatgg gcacctggca gagcgtcgca gaagaacgta cgcccgatga
    17461 tatgggcaat gtgaccgccg gtaatgccgc cattatggac tgcagggaca gcatcatctg
    17521 gaacgccgcc acggataaag ggatttccgt agctgctctg ggactctccg atattgttct
    17581 cgtagtcacg gaagatgccg ttcttgtcat gcacaaagaa cacgctcatg atcttaaaaa
    17641 actgctggca gaaatggctt caaagaaaaa aaacgtccgg cagccaaaac agaaaaaaga
    17701 cggggatgct cactgagacg cttctgtttc ttctgtgctt ttttttctga aagcgacgga
    17761 ttgcagtccc aatgtaagcc atccgaccgg aacccagatc cagacggaaa gaatgcggaa
    17821 agcgaggacg gccggaacca aatgcacagg gggcgccttc agccaataga gcagtgcgcc
    17881 aatataactt tcagccacgc cgaatccgcc cggtatgccc gaaatggcac ccacaaaaag
    17941 agtggaagtg ctgatcataa gagcatgcat atacgataga tcgggaatca gctctctgaa
    18001 gaccagatac aggttcagcg cattgatcag ccagttgagc atgcttaaga aaacaagtcc
    18061 tgccagagcc ggctggagaa aactgacaaa gccaatctgc agcttcaacc ggatgagcaa
    18121 gggccgtatt acttgagccg ccgccagtcc ggcagggata agcagcagcg gcaggaagaa
    18181 agctgtggca gaaatctgaa aaaacatgaa tgcggataca aagagtgccg aagcggaaat
    18241 cgccgtaaaa cccagataaa gcacttctgc ggaaaaagta gttgcggctg aaacgtagca
    18301 gcgccgtgct gtttcagcag aacggaatgc gcgaccaagc tgggcaggca tcagcagagc
    18361 ggcatgggct gaaaaataca ggaaaatgct ttgcaccttc cccggcgtga cgccctgatg
    18421 tcgcagcacc tgccgccaga tcagcccatc cagaaaaata tcgcaacagc caagcaggaa
    18481 tacctgagcc agcaggaacc ggcgggcatg ccagaaagca tacacagccc cccagtcgct
    18541 cccccgcagg ctgtacaagg cataaagggt aatgcccgca ataagacaca gcaccgcagt
    18601 gcgcagttta tccgcccaat accggaagga cctgtgctgt tcttctgcct gcggggcgga
    18661 ttcattttcc tgcatgcgga tctttacctt ttgtgtactg cacatacacg gcgctgtttc
    18721 ccgggatgtc tgcttctcct gctacagaca ggataccaga tacagcacgg ctgcagcacc
    18781 cacggacgcg ggctttttaa aatactgttc gccgccggac agtgtttttc tgcctccatg
    18841 aaaccaccgg ccgctgctga tgtcaaacca ctgaagcgta tagcagcctg cttgcagatc
    18901 aacacccgga taccggccgg tttcactgta gatgaggtag agctgccccg gcctggcaag
    18961 aacataacgg ctgtcacccc gcgccagcgc atcagaaggg gctgtttcat taaaccgtac
    19021 cgactccatg aatcgcacca gtccgccgca aatcttcaga tcctcttctt ttgttttgtc
    19081 tatgtccatt tgcaggatca tagaatatgc cccgccaaag gcgatggacc agatggtttt
    19141 ctgcagcgca agaccggttg ggcggggctg aaattcggac aaattaatgt ttttccgccc
    19201 tcctgtattc tgccacgccg aaaccgctgc ttcatgaagg ctgtcactgg atgcggtgtt
    19261 gaactgcatg gcaaactgat ccacgtggat gttgtcggta aagtcaaaaa ctgttccatg
    19321 attctgatgt accgccacag gatgccgcaa cggatcctgc gctttgatgc gctcagcaat
    19381 tttcgctgcc cgggcgggag atactgcctc gatatattct tccgccacac accagataag
    19441 atacgggtga cgatggaacc gcgcaacaac agtatctatg aactgctctt cccggggatc
    19501 cagggcacca tccgtcagat ctctgtgaaa aggcgccgca ctgtcatcat aaaaaaagag
    19561 aaaaataacg atgccctgcc tgttcatctc tgaaaaccac tgatcccatt gatccagaat
    19621 agccgtatta agtccctgcc cgggatcgcc attaacaaaa gggttttcat cggcagtacc
    19681 gtcaccgccg tggctgcgta ccatgatcat ataaatgcag ttggcaccgg tgcccgccat
    19741 ttttctgatg agcgcctgct gatcgccctc ccgggttccg tcagccttgc gcagaccccg
    19801 atataaaaag ccttcaggat ctccaggacc gcacatgtaa aagggagcat tgtcaccata
    19861 gagaagccac gccggatgct ccgggtccac gttaatggcg cccctatcgc aggagaaggc
    19921 atctcccatc acactcgctc ccagagccaa caggacagat aatacataaa aaacataaca
    19981 atcctgcctc tttttcattt tatgcaccct cctgacgcaa aatgccttca tgttatttta
    20041 caccgcttgt cctatcggcg ccaagaaaac gtgcccacct gaaaagatat tttcttatat
    20101 atacactaca catccgcata aaccttcatg gaattcgcaa gtttgacatt ttacggctgt
    20161 ttgtctagaa tatgcccgat acggtttgtt cagttctccg gttgtggcgg gtcattcttc
    20221 aggcagcttg tcggatcaaa ccggagaaaa actgacacat ttacagtccc ggcatgtatt
    20281 tatacttaaa ggaaacatac cgttatgaaa ctgaatgctc ctgttatcag ccgtcttgcc
    20341 cttcttattg tgtgcgctgt tgccctcagc ggatgccatt tcacaataca gcgggaaaca
    20401 gagattattc ttacagagaa tgttaccgtt tcgggagcca accggataaa caatactaac
    20461 ggcactctga cagaaaatga tctttacgaa gaagtgttgc tgcaaggctg gaaaaacggt
    20521 ctggtcactt tcccgctggg aacaaactgt gattttacca aaaacattct ggaacttgca
    20581 gaaaaagtga aagatcctgt gcagtttctg gacagcccct tcgcgcctct gctcaagatt
    20641 ttctttgtac agggcaaatt ggacgggctg gtgcttacca aaattgctct ggaaccttct
    20701 tccgggaatt tcgacactat cacacgatgc tctctgcggg catttgacga aacccagggc
    20761 accatcaact cctacagtgc cgcagggcct tttcagggta aacccgttct gacacctgac
    20821 cgcaacattg atatctaccg ggtcattcgc gatgccggag tcgaatgcct cacaagcgaa
    20881 atgactatgc agggctcccg gccccaaaac gaccttattt tcactgcaaa agcaacagcg
    20941 gtatatgaag ttaaattgcg ctttttcagc ttctgaaaga gcagcagcgc ataaaacaac
    21001 gccttaagcc cactcgaagg agaagcggat ttttcaatcc cttctccttt ttctgtttca
    21061 gaaaaacaac gcctgtcccg cctatattta tcttttgaca aataacgatt tatgtgatat
    21121 gttttagaaa agcgcagttt gtttcagatt gattgattta tgaataaata cacaacacta
    21181 ttcgctaatt acaaagcaat actttcgccc atacaggtgt tatacaggca aataatagta
    21241 ccacggaaag caccctcgta aacccggtct gccagcggta tgatcttttg ttttttttgc
    21301 attgaattat gagtaattta ctaatgatta cttttcgaaa gtttcctgtc cggaacagta
    21361 cccggctctg aatcttgtaa tctgtacttt gcatgttcgc aacttatttt ttcgagaaaa
    21421 gacttgttaa caaaaaagac tgcccttatc cttggtgcca ttttggttgt catgccgcta
    21481 tccatcgcaa cagcgggaac ctacagcgga ggcagcggca ctgctgacaa tccgtatctg
    21541 ctgagccgtg cggctgactg gcttgagttg tctgaaacac aaagcgactg gggcagcgat
    21601 ttcaggctga cccgaagcat tgatttcagc ggtgtcaccg taaagcctgt aggccgggac
    21661 tttgatcccg ccaaccctga ctacccgcat atttacttca gcggcagcct ggacggcggc
    21721 ggatatcgcc ttaaaaacat cgtgttaaat tatcctgatg aaaacgatgt tgcgcttttt
    21781 ttcagcatgc agcgtgcctc cgtgaacaat ctgattattg aaaatatgga aataaaagcg
    21841 aaacggcagg ctgcctctct ggcagtctat tttgatgaaa gtgctgccaa agatgtaaaa
    21901 ataatcagca gaatcagcgc agaaggcgat gcaggcggtc tggctgtgga ctgcctgaac
    21961 tccaaattta tgcgctgctg gatcggtacg gatatccaat gcaccgacga caacagccgg
    22021 gcaggcggta ttgcagcgct ggaacagggt gaggaaattt ctttttcaga aagcatcgga
    22081 actatcgttg caaaagagcg ggcaggcggc ttcttcgcca cggcactgga tttggcaatt
    22141 agaaacagca cttcttttgc aacgacacaa agcgccatcg ccggaggctt tgtcggatat
    22201 gccgataaac tgactgtcac aaaatcctcc gctctgatag acgtgcgcgg ctacaattat
    22261 ggcggcggcc tcgcaggcta tgcccagaac agtaactttt tcaacacttt cacccgtggc
    22321 agtgtctccg gcaacaatgc ggcaggcggg ctggtcggtt tctgcgacca caatgtcagt
    22381 ttcaaagaga gtttttcctc tgttgctgcc agcagcgttc tgggcgattc cggaggtctg
    22441 gcaggggtat tgcgcaattc aactttaaca aactgttacg cccggggtca gataacgggt
    22501 gtcggctttg tcggagggat tgccggttcc gccggccagt caaccctttc caactgctat
    22561 gctgccggcg ggctcaccct ttttggtgcg aaccgaaatg caggcgggct tctggggtat
    22621 gcagacaata caagtctgga tttttgctac tgggacacag aagtatccgg cacggcatac
    22681 agtgccggcg gggacggaag aacaaccaat gcaatgacct atcctcacgc tgtgaatacc
    22741 tacacaaaat ggcggtttgg gattttctgg actgcagaca cggacagcac cgtaaatgac
    22801 ggtacccctg gctgcgcaat acccctcctg ttgcactcga agtaacggtt gagggcgagg
    22861 agcctccgga aggagaagag gctgcagaag gcgaaatttc tgaggacagg tacagcggcg
    22921 gcgacggaac agaagaaaat ccctttctga tcagcagcgt agaagactgg caaactctgt
    22981 gccggactca gaaagactgg ggagcccatt ttgaactgac ccgaacactg gacttcaccg
    23041 gtgcggaagt gagatcagtg gggtgggatt atgatcccga caatgagttt catccccttc
    23101 catcgttcac cggctcgctc aacggaaatt actttcatct gcggaacgtg aacctgaatc
    23161 gcccggatat gaacgatgtc gccttattct acagtctgaa gggcgccagg attcagaacc
    23221 tttttataga caatatctcc gtcgatgccc gtacctatgc cggtgcactc gccgtctaca
    23281 tttcctccag caccgtcagc agatgcctca tgtccggctc cgttcagtct ctggactgcg
    23341 ccggaggcat ggcaacatta atcagcggcg attcacaaat tcaaagctgt atccttcaga
    23401 caaacgtccg ggtcagaaat gagttgaacc ccgtgggtgg atcagccggc ggaatcacct
    23461 gtttcacgca ggatactgct tttctcaagt gcagcagtgc aggcaccatc gtcggaaaaa
    23521 ccgaagcagg cggattgacg gctcactccc tcgcctcttc tttttcagaa tgctatgcac
    23581 atgtaacggt gcaggcttcc gttgcaggag gactggcagc ttctgtaaaa gacggcagct
    23641 tcaccgattg ctatgcacgc ggcatcgctt ccggaaaaac atatgctgcc ggactcattg
    23701 ccaaaactga cggcatggtg ctcaacaata cggacaatgc ggaaatcaat cgttgctttg
    23761 cggcagttcg aacacgtgtt gacggagaag gcgaaatcgc tgccggactg atcgccagca
    23821 gcacaaacac caatgtcacc gcgtcatatt gggattcgga acgctcagga aacaatagca
    23881 gcgtcggtgg tgaaaggcgg acaacggcgg acatgtcttt tccctacgcc gccaactcct
    23941 ttgtcggatg ggattttaat gctgtatggg cttctgatcc cgactactcc aaaaacagcg
    24001 gctacccctg gctccgcaat ctgccgccga ccgctgtgat cctcctgggc gacaagtaca
    24061 gcggcggcga aggcaccgca gcaaagccct atcaaatcgc cagcgtcgca gactggcagc
    24121 aattgtgccg gtcacaggag gactggacaa agcattttga aatcatccgt gatctggact
    24181 ttaccggtgt aatcgctgtg cctctaggcc ggaattataa cagaatacac cctgatcacc
    24241 ctgttccgtt ttttcaggga acattggaag gaaatcataa acgcctcata aacctcagca
    24301 tgcctcaagc ggaggaggag gacgttgcct tgttctatca tttaaaaaat gcggtcatac
    24361 gaaaccttaa tatcgagcaa gtccacatag tttccggcga acgcgccgcc gctcttgctg
    24421 tatatctgga agacagcacc gtcataaact gctttgtaag cggcaccatc tccggcgatg
    24481 cctatgctgg gggggatcgc cgccagaata atgtcccagg gcagcctggt gcagagatgc
    24541 cgggcggatg ttgaaattca tacgacagcc gactacgcca ccgccggcgg tatcgccgct
    24601 ctattactcg acggcaccgt agagcagtgc agcagcgcag gaaccattca aggcaagtcc
    24661 tggtcaggag ggctcttagg aaatatcttt tccggaaata ttgccagatg tacatctgtc
    24721 gcacacgtca ccgggcggag cgccggcggc atggccggtc tgattgatcg tggaacctgc
    24781 agcacttctt atgcccatgg aagagtccag ggtgaagtag cagcgggttt tgcagcggaa
    24841 acggtctcaa gcgtgctgaa cgactgctat gcccgcgggc agacaagagg cgtacattgt
    24901 gccgccggat tcattgcacg tttaaataac aggctcaatg acaaagcgga taacgcccag
    24961 ataaaccgat gctatgccgc tgtacggctg aaattgactt cggacaatgg cattgccgga
    25021 gggctgatcg gcgaaaatgg cggtgccgaa gtcattacat cctactggga caaggagtat
    25081 tcgggcacac tgattagcgc aggaggagcg ggacgcacca cgactgagat gactcatccc
    25141 catgcagcaa atacttatat caattgggat ttgaacggga tctgggctgc agatgaaaat
    25201 ggcaacatga atgcaggata cccctggctg cgtaatctcc cccgcccgcc gcaggacgat
    25261 gaaaatgaca tcgaaggtga agaacctgat gtcgaaggtg aagaacccgg gatcgaaggc
    25321 gaagaacccg ggatcgaagg cgaagagcct gatgtcgaag gcgaagaacc cgggatcgaa
    25381 ggtgaagaac cagaggtcga aggcgaagtt tctattaatc cggagaaaaa aggatgcgcc
    25441 ggttgcggat gcgcctccaa agacctccgg acttctgtca aagacctgct gggcgactgg
    25501 ctgctcgtgg cactttgcct gagtgtcctt tttatgagca cattactacg tcgaaaagtg
    25561 taaagtatgg cataattaag ttacatgctg aacagacatg gacggaagaa tttctgcttt
    25621 caaaaaaaac gtcaggaaac agaaagcgtg tccatgcgca gcctttacat aaactccggc
    25681 ggagagggat cttttttcct ctgaggatcg catcgttcaa aaacaagtca caaaggaaaa
    25741 cagacatgaa aaaaacaatg ttccggatgc tttttctctt gagtcttaca acagccatgg
    25801 cgctcttcag cggctgccac tatgatcttg atcagaccgc aagtttcgat atcctgtcga
    25861 aacctgtgct gctggataca gcgctgctcc agacgcttcc gggcggtgaa ttcaaactcg
    25921 agctggaaga aaactgcgag atcccggcgc aggtcagagc tctggtgaaa aaactgcagg
    25981 atcagattac caaccaggaa attgacctgg gcgcctatgg attcctgccc ttcattaaac
    26041 caaaaattga agtccgtgcc ctgcatatca acgaagttga cctcaatgcg acaaaaaaca
    26101 ctttcactgg cattaatgca ctgagtttcg aaatcaagga cagttcccag ggaaaatcca
    26161 tccgcttgca aagtcaggaa atcacccctc aacagattcc tttgaggccg gtagaaaata
    26221 tggactggct ggccttctta atgtcgccgg atatggagtg tatggaaact tctctgtcct
    26281 ttacaggcac tccacctgaa aaaccggttt cctttacagc ccgtgcacat gtgacagtaa
    26341 aagtcaggat tttacttttt taaacggaaa aaacctgtga attcacctca cacaattcac
    26401 aaacttgaca caaattgcct tttgctgata aaataagcaa tcttttcagt ttcccctaaa
    26461 ttatctgtta agcagtgaca ggagtcgctg tcttactacc cacgcccgtt gagggacgta
    26521 atctgcccag gagggaggtt gagttttagt gactaaggtt cgtgtcaaaa ccgatgaacc
    26581 ctttgaaaaa gcattgcgcc gattcaaaaa gaaatgcaat aaagaagggc tcatgcagcg
    26641 gctgaaagag gtaacctact acgaaaagcc gtctgaaaca cgtcgccgcc gtctggcaaa
    26701 agccgtcgcc cgcgccgtga tggaagaact ctaatccctt ccccgctctg taaacacgga
    26761 ggcagacatc gcaagatgcc tgcttctttt tttattttac aggaaaatta tacttccccg
    26821 acctctgctt ctgcttgctt ctgttcctcc tctacaggcg gattgaagag cgcagcgagc
    26881 cgatcccttt cgatggcggc gcggttgatt ccatccatgg cagcctcccc cgccctgcag
    26941 gcttcttcat gatagccaaa acggttttca cgcagaccaa aacgcttcaa gagctcttca
    27001 taacgggcaa gcattgattc gggcagcccc tttacacggt gtgccacctt ccgtgcagga
    27061 ttgcccgccc atacctcaag aggacccacg tctttcgtca tgacgcgctt gtgtgccaat
    27121 gcggagattg gcgttcacag gggaagaatt gcctccctcc gtcattcctg ctcctccctc
    27181 ccgtcattcc tgctcctccc tcccgtcatt cctgctcctc tctccgtcat tcctgctcct
    27241 ccctccgcca ttcctgctcc tccctccgcc attcctgctc ctccctcccg tcattcctgc
    27301 tcctccctcc cgtcattcct gcgaaggcag gaatcttaat caggatgtgg acgtctcgcg
    27361 ccac
//
LOCUS       DAOPWB010000028        27250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1743_length_27250_cov_5.068101, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000028 DAOPWB010000000
VERSION     DAOPWB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 27250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27250
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_27250_cov_5.068101"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PKN92_04270"
     CDS             <1..1217
                     /locus_tag="PKN92_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ48041.1"
                     /translation="SNAPHSAGSTFDLAFTQPLLRGAGASVAAERLTQAERDALYALR
                     KFTHYRKQFTVDVCRDYYRVLQQRDVVRNTWVGLDSFRMNVERERAFAEENLSTQAEL
                     GRMIQFQLDNENRYINAARQYQEELDSFKILLGLSTDTALVLDNTELDKLRHSELIHP
                     AVSAEDAVKVALESRLDYKNETDQAEDALRRLKVAANALKPGVNLVSRASVPTTGESR
                     PLHFDLERMDWNLGLDFDAMVDKKAERNAYRAALIDVERSARQASLAEDTVKLEVRGA
                     WRTLEQARRNFEVAQESVRLSERRVEEQQLLSELGRATAQDQVDAQNDLIQSQNNLTS
                     ALIAHTMARLGFWRDMGLLYISNEGLWTEIELNGHTGGVDPGVAVPEETDAENEGTEK
                     EDGAEEHMLLQL"
     gene            complement(1320..1396)
                     /locus_tag="PKN92_04275"
     tRNA            complement(1320..1396)
                     /locus_tag="PKN92_04275"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1360..1362),aa:Ile,seq:cat)
     gene            complement(1503..3263)
                     /gene="rpoD"
                     /locus_tag="PKN92_04280"
     CDS             complement(1503..3263)
                     /gene="rpoD"
                     /locus_tag="PKN92_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNZ48042.1"
                     /translation="MSKETKIDEIMTNPKSSRILKNAQARGKLTYNEMMLLLPDDADA
                     MLMDDLTIKLMQTGITVVEELNPKALQEKKQDKLAEEARLLEQRRLATQRSLDRADDP
                     VRMYLREMGRVPLLNKDQEIEIAKRIEAAENELISVILDAPFTIKETQMLAARILAGR
                     LEFCHICENTDTKFHHKFVKELPQYMEKMGALDVEIEKQEKRMRRAGLSPKSIQNIED
                     RIREFRKQQVDIIRSFQLKNREILKIGRKIKSLKRRLENAFDEVDRIEREIGMSAEEI
                     LKLGTQSRRNQSSFKKHALDRDNFLELERRLLIAQRKINQIQEGARLDKGKINELIAV
                     IIEKEDNIYEAKMELVQANLRLVVSIGKKYINRGMSFLDLIQEGNIGLMKAVDKFEYQ
                     RGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIESINKLMRTSRNLLQKLGREPTA
                     EEIGAEMGMSADKVRAVLKISQEAISLETPVGDDGDSSYGDFIPDMEADNPSHKAGTA
                     VFRERLDKVLATLTEREEKVLRLRFGLADGYPRTLEEVGSVFNVTRERVRQIEAKALR
                     KMRHSSRAKELKPFENISNQ"
     gene            complement(3327..5087)
                     /gene="dnaG"
                     /locus_tag="PKN92_04285"
     CDS             complement(3327..5087)
                     /gene="dnaG"
                     /locus_tag="PKN92_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNZ48043.1"
                     /translation="MKKYSRETIAQVLAANDIVDVIGESVELKNAGASRFKGLSPFTN
                     EKTPSFMVSRDRQMFYCFSSSQGGDLLQFLMQFEGISFNEALEKLAARAGIHLEASGK
                     EDDREAYQRRRILELNSFALRFYRQQLKSPETGTAAQEYLKKRGMHPATEAHFSLGLA
                     VDSFSTFKDAALKEGFREGELIASGLVRQGEKNIYDFFRNRLIIPIRDISGNAVAFGG
                     RDLGDSPAKYINSPETSVYKKSRTLYGLFEAREAITREKTAILVEGYFDLMRCAENGI
                     ENVVATCGTALTEQQAMLLKRYAPKVVVVYDGDTAGVRAALRSIALLTGAGLAVHAAA
                     PPEGQDPDDFIRDHGADAFRNLINTAPDFVSFYIAMQGEQKNTIEGRTELAHTLFSIL
                     RVIDEPIRLEQYLNLVAEALNIHVWECRRNFERFIKSRRFPEQRADFNEKSNEETLKT
                     TKDDIDFLAALLEYPSLRIKAAALDTELELSHPVLSLARLINSRKEEKINVQDLPEGE
                     ERNLFSAAAVAEAPPPEKAERLIEERLARFAFESCAQKMNRLQQEVRRATQDQDAARS
                     AELYKRIVELKQFMESLATS"
     gene            complement(5226..5945)
                     /gene="rsmI"
                     /locus_tag="PKN92_04290"
     CDS             complement(5226..5945)
                     /gene="rsmI"
                     /locus_tag="PKN92_04290"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNZ48044.1"
                     /translation="MSTLYVIGTPIGNLEDLSYRAVRILGEVDAIACEDTRITRKIYE
                     RFQIPSPAIRFSSHEHNEDQAAKRIIGLLREGRSVALCTDSGMPCISDPGYDAVNLCR
                     EAGFAVELVPGPSAVLTALVLSGLSGASFTFKGFPPRKEGKRKRFLEMEKDAPHTLIF
                     FESPFRIAVLLRDALEVLGDREAAVCFELTKHHERVLRGPLSELYARFDGAPHPKGEI
                     TVVIAGNNPKFLHKDEPSEND"
     gene            complement(5942..6949)
                     /locus_tag="PKN92_04295"
     CDS             complement(5942..6949)
                     /locus_tag="PKN92_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNZ48045.1"
                     /translation="MACESTAYARAGLVGNPSDGYYGKTISLILRNFAAKVTLYENPD
                     IEVVPSFQDRMKYSSMQELSRDVENNGYYGGIRLMKAAIRRFWLYCRDQNMPLPDRNF
                     SIRYRSTIPRRLGLAGSSALVTATIRCLMEFYDIDIPKPLLPGLILSVETEELGIAAG
                     LQDRVIQVYEGVVYMDFQRALMEAQGHGNYEKLNPALLPPLFIAYREDLGEGSETIHQ
                     TVRDRWLAGDAQVLETMEALGECARQARDLLLAGKGTEIGPLFDRNFELRCSIFKVDQ
                     RNKDMITCAKALGAHAKLAGSGGAIIGIYEDEAMYQALAEAFQKEGIAIFKPQIEPEQ
                     S"
     gene            complement(7048..8265)
                     /locus_tag="PKN92_04300"
     CDS             complement(7048..8265)
                     /locus_tag="PKN92_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNZ48046.1"
                     /translation="MPTFQYTGRLQSGMKRTGSIVAEDREDAVRQLHAMGIEPDRLVA
                     GQSNVGRTASKLNRKVKSHELLIFTRQLATIVSSGLPLMQGLDILAEQTEDARFSAII
                     DSIAQEVEAGETFSDALRKFPRAFPDLYTSMVRAGEASGDLDGVLLQLADYLEASEEL
                     KRRIRSAMTYPVVAFSMIILIATGLIIFVVPQFAEIFGSFGAELPAPTQILITTSNFL
                     RQWYSILMIIGAIVGIVTAVRIYGATPGGRYNLDNLKLRLPVFGGLLRKVAISRVART
                     LSTLTKSGVAILQAMEIVERTAGNEVYAKAVREAGECVRNGETLADPLTRSGVFPAMV
                     TRMIGVGEKTGALEGMLMKISEFYDSEVKATVDGLTSLIEPILIGFMGTVVGGIVVAL
                     FMPILQMSQLINK"
     gene            8616..9854
                     /locus_tag="PKN92_04305"
     CDS             8616..9854
                     /locus_tag="PKN92_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ48047.1"
                     /translation="MAQVRVCALAVFFVSLAAYGVDCPQFRGLDRTGVYPDTGLLQRW
                     PEGGPPLLWESSGFGVGFTSPLIVKDRIYVTGTLADQESFIFVLDQAGKEIERIGYGI
                     ETTAESAPGSRCTPTIDGSDLFILSGPGVLSCIDLSASSVRWQVNILEKFNGPQIEWH
                     LSESPLVDGDRVICTPGGADAAMVALDRKTGDTVWVSHGLTDMTSHVVPLLVEHNGRR
                     LVFTETAKYLICVDAEKGTLLWKREHETEWDIHAVTPIYRNGQLYYVAGYKSGGGLLE
                     LSEDGSSYTVKWLDTELDCQHHGVVYLDGFLYGTSHHRGGGRLVCLRWDTGEMMWADK
                     AVRQSSIIAAEGMLYAYEDSRRGTMRLIKASPEGLEVRGEFSMPGGPKEHWAHPVIAN
                     GLLYMRHGDTLRCYDIRESR"
     gene            complement(9869..10579)
                     /locus_tag="PKN92_04310"
     CDS             complement(9869..10579)
                     /locus_tag="PKN92_04310"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNZ48048.1"
                     /translation="MAETSAPKCTVPRKNKITHTFRKPLISGLLIVVPMGVTAFVLKL
                     LYAFTAGRLSPFVRKYIDPVPDYTSPVIASILLFAFIYFMGLIASVVVGKKLIALFEN
                     LIQTIPFVKSIYKASKQVVESLAFAQEDTAFKIPVLIEFPCPGMKCIGLALGTIRCRD
                     GREYYNVFVPTTPNITVGLYLLVAPEDVYKCDLTIDEAVKIIVSGGILGPEQMDLTSA
                     AITPLGAPMEEEEDDDEE"
     gene            complement(10572..11288)
                     /locus_tag="PKN92_04315"
     CDS             complement(10572..11288)
                     /locus_tag="PKN92_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNZ48049.1"
                     /translation="MSQEKNKKKRKKKKKRPGLKAILQHRILSGLLVVVPLGLTVFIL
                     RFLYDFTAGRLEPVTRVLFTKVPEYGIAFVSILILLAATYGIGLIAHIVIGKKIIALA
                     EGLLSKIPFVKTVYGASKQVVESLSLQDDANSFQSVVLVDFPCPGIKAFGFVTNTVVL
                     RDTVSGEEKKHYRVFIPTTPNPTSGYLEFVPEDRAENAGISVEDAITTVMSAGLISPK
                     MIGEPAGYSPEARHEDMRHG"
     gene            11715..12788
                     /gene="purM"
                     /locus_tag="PKN92_04320"
     CDS             11715..12788
                     /gene="purM"
                     /locus_tag="PKN92_04320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNZ48050.1"
                     /translation="MTEEKRSLTYSDAGVNIDAANTALSTVKDLVRRTYTQHVLRDIG
                     TFGAMFQLDLQNYREPVLVSSVDGVGTKLKLAFMTGKHDTVGVDLVSHCVNDILVQGA
                     KPLFFLDYFAMGKLEPDVMVEVVRGLATGCRYSGCALIGGETAEMPDMYTVGEYDLAG
                     TIVGIVDRSRIVDGSAIRAGDVIIGLPSSGLHTNGYSLARKICFEVAGLDVHDPMPGT
                     GRTVAEALMEPHISYAKLMQVIMRTVDVRGMAHITGGGITDNLPRILPDGVAAEIRLD
                     SWTVPPVFRFLKEAGNVDDKEMLRTFNMGQGFLFVVPEEHAARAKETIELAGQSCSFA
                     GRIIEGDGKVHYSGNLNYANTSN"
     gene            12769..15735
                     /locus_tag="PKN92_04325"
     CDS             12769..15735
                     /locus_tag="PKN92_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNZ48051.1"
                     /translation="MPTHRIEVALRPEFKDPAGEALRAQVREDLNLEIGAVRVIDVFT
                     LHAALTAEELERIRMDLFTDPIIEYSAVNASLAENFSCLIEVGYLPGVTDNAGRSAQE
                     GIEDSLGRKLAQGEAVYKSTLYVFQDTDAETAKTIASNLLANELIEQYAIRTPEEMKA
                     LAGKSLLALPRVVEYAVPEVESIDLNLSDDALQQLSRDRMFALELGEMQVIRDYYNRA
                     DVQAHRRSIGLPDKPTDIEMEILAQTWSEHCKHKIFNAVIHYDEGNGKVRVIDSVFKS
                     YIKKLTDEVGKRVPWLVSVFDDNAGIIQFDENHLFALKAETHNSPSALDPYGGAMTGI
                     VGVNRDVLGAGLGFQCIFNTDVLCVASPFHDEEIPPRLLHPRRILRGAHAGARDGGNQ
                     SGVPNVNGALVFHERFLGKPLVFCGTGGLSPAYIQGRPAHKKAARPGDAIVMTGGRIG
                     KDGIHGATFSSEALHEGSPATAVQIGDPITQKKMSDFLMEARDAGLYNCITDNGAGGL
                     SSSVGEMAEKPGGAVIHLDKAPLKYAGLAPWEIFVSEAQERMTLAVPQETLGRFLDLA
                     ERRGVEATVLGAFNDSGYLECFYKGEQVASLDMDFLHHGLPRMELEACLAPSPDPVEV
                     TAGEGKSDLTDDLLSLLGSLNICSKENFVRMFDHEVLGQSVLKQFQGVQHDGPGDAGV
                     IRPLPDSYRGIAVSCGISPKYADLDAWSMTACAVDEAVRNLVAVGAAMGTIAGLDNFC
                     WPDPVQSDSTPDGKHKLAQLIRSCEALYEICLTYDIPLISGKDSMKNDYRIGDTKISI
                     PPTLLFTAAAIIEDTRRVVSVDVKQAGDRVYVLGDTLNEMGGSEYFTLQQISGGKAPT
                     VDPKKARTLYEALNRAIQGGLVASCHDCSDGGLAVALAESAFAGNLGMDLSLEALGVE
                     DGVTALFSESQSRFVVTVSPDHAEAFEAVLAGSPCYCVGVVTEEPLFQINCPDGRKIS
                     ASLDVLRSAWKKTLNW"
     gene            15752..16543
                     /locus_tag="PKN92_04330"
     CDS             15752..16543
                     /locus_tag="PKN92_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNZ48052.1"
                     /translation="MTVKTLVLTGFGINCDQETHQAFACAGSDVTRVHLSDLLETPAL
                     LDGAHIFVVPGGFSYGDHVSSGKILANRLRHQLEVPLRQFVEKGKLILGICNGFQVLA
                     KMGILPFFDGTVSQEVTLTTNESGRFENRWVHLAVPENTPCIWLKGITKFELPVRQGE
                     GKLIVRNDAVRDRLHKEGLVALRYVRPDGAPAAGVFPFNPSGAEDDIAALCDPSGRIF
                     GLMPHPEAFLDGMNHPEWTRLGLAEEGEGLALFRNAVEFARTSLC"
     gene            16574..17311
                     /locus_tag="PKN92_04335"
     CDS             16574..17311
                     /locus_tag="PKN92_04335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNZ48053.1"
                     /translation="MSHLHRFFSSLPEEGKASFLLPPEEAHHALRVVRLRDGDTVSVF
                     DGRGGEVEGRLRPCGKKEAEIEIVRRMQHPEPAVKMTVAVAWLHRDKAHEDIVRRAVE
                     LGMWRIIFWRADHSQKKCAAADRWEKIALEACKQSGRVFIPEIELQDSLDALFETVSR
                     PVIHALPAADPLQKPEILVHDQVTMLIGPEGDFSDREVALAQEQGAIQIHMGNCIFKS
                     EVAAAVAMTLLAAQVGELGRHFSIVSG"
     gene            17331..18560
                     /locus_tag="PKN92_04340"
     CDS             17331..18560
                     /locus_tag="PKN92_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ48054.1"
                     /translation="MKILFLCQYFPPEPGAPAARTHEHAREWVRQGHDVTVICGIPNH
                     PDGQVPRSYAHEYVYDELYDDIRVLRCWFFVTPNRGRIRRSLSFASFMLAALFWGIVR
                     GGRADVVAATSPQLLCGAAGYLLSLFKRCPFVFEVRDLWPDQIVDLGLIRNTAVIKGL
                     MKLEAFLYRKAAAIVTVAEATRRNLIGRGIPPDKVHTITNAIALDIFTPLPRVGVMRH
                     RYGWDDTFLVMYIGTHGISQGLETVLEAAEKLQSASGLRFVFVGSGAERMRLKERAEK
                     LGLTNVEFIPSQPRGDMPEFYAAADLCLVPLKKRNVFLTNIPSKMFEIMACARPILLG
                     AEGQAKEVLEQAGAGVAFAPEDADALAAAICALKDDPEKCRRFGESGRHYVEQHCNRA
                     LKAKEMLAVLEEVQQRK"
     gene            18699..19568
                     /locus_tag="PKN92_04345"
     CDS             18699..19568
                     /locus_tag="PKN92_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HNZ48055.1"
                     /translation="MARFRFLPCLDSDEMAASRKLTSWIDRDLARSLGERAVVAAEDG
                     FYFLEDGSKVAWRTQVERARASRISIAPEAPLPLHVVEPVEETVIQVANKTTLQAGRE
                     LVDAGHRPLALNFANGITPGGGFLSGARAQEEGLCRSSALFVTLQNDAMYQFHRARPR
                     PDSAEWCIVSPDVPVFRDDAGNALKETWLLSFITSAAPYAPSIGQPESGDLLKKRIRR
                     VLAIASAYGYKALVLGAWGCGAFGNDPQRTAEDFHEILTTEFCGHFSNIVFAITDWSP
                     ERRMLGPFRDVFQ"
     gene            19747..20925
                     /locus_tag="PKN92_04350"
     CDS             19747..20925
                     /locus_tag="PKN92_04350"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNZ48056.1"
                     /translation="MLTVWLTLALCGQLPGETMYYAEDAEHVHVYYEKGRFGGWPANH
                     GMWCWGNEILVGFSRGYYKDLGPTMHHIDREKPEEFWLARSLDGGQSWSWEHPAEKGF
                     LIPRGKALHGTETPGLSLPPLREVETPINFQHPDFALTFRMDNIDGGTSRFEYSYDRG
                     RTWEGPFRLPDFGTSGTAARTDYIVNGEKDAFIFITVSKSNKQEGRPLCARTQDGGIT
                     WKMVSWIGSEPKGFAIMPSTLRISDTELFTTLRCREGTFRWISAWRSPDNGYTWVQEE
                     NPVDYLGEGNPPMLNKLQNGRLCLTYGFRAYPFSIRARLSEDNGKTWSPQIVLRDDGL
                     DRDIGYVRSVQRSDGKMVTAYYISEKSTGPERYIGATIWDPNRISPITSGDQKRQAGF
                     "
     gene            21055..22212
                     /locus_tag="PKN92_04355"
     CDS             21055..22212
                     /locus_tag="PKN92_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNZ48057.1"
                     /translation="MNDQLSQTEDQEIIQSEAKPPAASARIMSVDVLRGFDMFWLVGG
                     AGVALGIGKLIDPKVHAFIDRQLSHADWEGFYFYDLIFPLFVFLAGMSLSFSMGRTLN
                     EKGLGATLIRLTRRCLLMFLLGVIYNGGFTNVWPEVRIMGVLQRIALSSLFAGVLFCF
                     FDWRGLAASLAALLLGYWALLSFVPLPGQDSISWAVEQNWAVWLDAQILPGRLIYKIY
                     DPEGILSTIPAIGTCIIGILTGLFLKRKEVEPLRKGLIFIGAGLVLVAAGYLWSYQCP
                     IIKKIWTPSYVLVAGGWSILLLALFYFLVDFWQIRWWTAPFLWIGVNPLTVYMARNII
                     GFNDLAKRFVGGSIYAFLGENLGYFVQCSVSLGIALLFVWYLNKKKIYLRL"
     gene            22245..24989
                     /locus_tag="PKN92_04360"
     CDS             22245..24989
                     /locus_tag="PKN92_04360"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNZ48058.1"
                     /translation="MTGRTVLYAAVLWQLFCTSVFAAAPLQERFANPPADTRILRIIH
                     HLPATEELRAHLYNRFAAMGFGGMATNVSFDSYLADEEKWTAFTEGVKEAKARDFSLW
                     LYDECGYPSGAAGGITLEGHPEYEARGLHIYETAGAEKDPTVPVPAGRVVYVLGIPDE
                     TAVVDLAAVTDLSGLVAEGQLRWADAPDRWRILFCMEDELYENTHAAISLAFKLRYIN
                     LLCPEATRRFLEVTHDAYAARLDNRLQDYFVSTFTDEPSLMSLFMKRSDHRVLPWSDA
                     LPQLFESRWGYSLKEALPSLLSDTNGRDGKVRHDFWKTIGDLVSENYFGQIRRWCDDH
                     GILSGGHLLCEEDFLAAIPFYGNFFQCARELTAPSIDCLTSIPDEVPWYSARLLSSVA
                     ELDNRQITMSETSDHSQRYRTEEDSRPVYHVSEEEIRGTLNLLLLNGINTITSYYVFK
                     DLSDEALNRLNEWTGRCSTLLRDSRQVTDIALVYPVESAWARFTPARFWTADSPAAAH
                     HVAKCFHDAEENLFCARRDFIHVDNETLEQCRVESGVLAYDALCWRVVILPDADTLSR
                     AAWQNLYRFYEEGGVLIALSSRPENSTEHFPDEDVRSLGRVIFGEENRPCVRIHEKGG
                     IGIFLPPGSEALLPEVLDRLIPPEIKLSEGENLRVTHRRLPDCDLFFIINNSSEAWKG
                     EAVLPVTGAVDAWDPATGSRTRFAAGKPVSLVLDGYSGIFLLCEPCSVPERSPVKEGV
                     LPDFVCEPLPEVSPATTAGEDVDVSLQRVMLPEEEAAGWHLEGRLTADRVNTFLFAGF
                     VHEPPVDLTDSVSLCFTAALPEKQQAAIPLLVILIDTAGVEYLAETGIPMSGSGIHHC
                     QVPLRRFRRAGWCSQVDKALDFSSITTIRIGWGGYYGPEGEVVRFSVSALGKNRIPEL
                     "
     gene            complement(25004..26212)
                     /locus_tag="PKN92_04365"
     CDS             complement(25004..26212)
                     /locus_tag="PKN92_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ48059.1"
                     /translation="MFSVPLRYQIVLYYCALACVALGTGMHDSTFNNFLADTFKLTAD
                     ARGHLEFPRELPGFLVVMFTGLLAMLSLTRVGLVATCTSALGLFLLSKSGTSYPVVIL
                     GMLGMSIGNHLLMPVGASIVIGLSSEKKRGTRLGQAAAVQTLFTAIGTGSVWLFMDKI
                     APQYQRNFAWAACAMLLGALFYTSMNIPNLRTPRQRLVIKKEYRLYYLVEFLSGARKQ
                     IFLTFGPWVLIKVYGLPAASIAGLLMLSCLIGVFFKPLAGMAMDYFGERTVMILEGVV
                     LALICLGYGYAQRVIPDPRWALRLACGCFILDEMLFALGNARSLYLSRLTPSIQELNS
                     SLAMGVSINHVASMVIPSIAGAIWVGLGYERLFLSAALFAIVLAFVASHVPRAGTLYE
                     RKLAKAAQTA"
     gene            26510..>27250
                     /locus_tag="PKN92_04370"
     CDS             26510..>27250
                     /locus_tag="PKN92_04370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ48060.1"
                     /translation="MDSTDFIDESRPLSRDQQYWQLRILISTYFGYAGYYLVRKVFTI
                     CKSTLALPVAEGGYGMGFNAVANIWTAYLVAYMLGMFVSSFIGRKWGPRVLLLGGLAL
                     SMLCNVIFGFANSYPVFVVFMFFNGLVQAAGWPGTVGAVAEWLRKKERGAIMGFWSTN
                     YLVGNILVKSLGGFLLATYSARYSTVIGVRYSFLGCTLLAFAFWWLLFFWQRNKPEDA
                     GLPPIVSSGADEARSVEASQSKSVTFRQY"
BASE COUNT         6014 a         6779 c         7743 g         6714 t
ORIGIN      
        1 gaagcaatgc gccgcacagc gctggaagca cctttgatct tgcctttact cagcctcttt
       61 tgaggggcgc cggagcgtct gttgctgccg agcggcttac gcaggcagaa cgggacgcgc
      121 tgtacgcact caggaaattc acgcattaca gaaagcagtt caccgtagat gtgtgccgcg
      181 attattatcg ggtactgcag cagcgtgatg tggtgcgcaa cacctgggtg ggtcttgaca
      241 gctttcgcat gaatgtggag cgggaacgtg cttttgccga agaaaacctc agcacgcagg
      301 cggagctggg gcgcatgatt cagttccagc tggacaacga aaaccgttat atcaatgcgg
      361 cacgccaata tcaggaagag ctggattctt ttaaaattct tctgggtctc tcaacggata
      421 cagcgctggt gctggacaat accgagctgg ataaactgcg tcacagtgaa ctgattcatc
      481 ctgctgtatc ggcagaggat gctgttaagg ttgcactgga atccagactg gattacaaaa
      541 acgaaaccga tcaggccgaa gatgcgttgc gccggttgaa agtggcggca aatgcgttaa
      601 agcccggcgt gaatctggtg tcccgtgcat cagtgccaac gacaggcgaa agcaggccgc
      661 tgcattttga cttggagcgc atggattgga atctcgggct tgattttgat gccatggtcg
      721 acaaaaaagc agagcgcaat gcgtaccgtg cagcgctcat tgacgtggag cggtccgccc
      781 ggcaggcaag ccttgcagaa gacacggtaa agctcgaagt gcgcggggcc tggcgtacgc
      841 tggagcaggc acgcagaaac ttcgaggtgg cgcaggagag tgttcggctg agtgaacggc
      901 gtgtcgaaga acagcagctg ctctccgagc tgggcagggc gactgcgcag gatcaggtgg
      961 atgcgcaaaa tgatctgatc cagtcacaga ataaccttac ttccgctctc attgcgcata
     1021 cgatggcacg gctcggattc tggcgggata tgggcctcct ttatatcagc aatgagggac
     1081 tctggactga aatagaactg aacgggcata ccgggggtgt ggaccccggc gtggcggttc
     1141 ctgaggagac tgatgcggaa aacgagggga cagagaaaga ggacggggca gaggagcata
     1201 tgttgctgca gctgtagtgc tagggcaagc agcgaaagca cgcttttccg ccctgccagc
     1261 tgcttgtcaa aaagaaaagc gacagtgcgt tattgcactg tcgccggggt agtcatggat
     1321 ggtgggccct gcaggactcg aacctgcgac caagtgatta tgagtcacct gctctaacca
     1381 actgagctaa gggccctttt tcgtgcatag tatacacctt caggcggggt acgggtcaac
     1441 cgcacagggc tgaaagaggg tgagaagacg gcattcccgt tcctgtttcc cttatctgtt
     1501 ttttattgat ttgaaatgtt ttcgaagggt ttcagttctt tggcgcgtga gctgtgtcgc
     1561 atttttcgca acgccttcgc ttcgatctgg cgtacccttt cgcgggtaac attgaacacg
     1621 cttcccactt cttccagtgt cctcgggtag ccgtcggcga gtccgaaacg caggcgcagc
     1681 actttttctt cccgctcggt aagggtggcg aggactttat ccagacgttc ccggaagacg
     1741 gctgttcccg ctttgtgaga tgggttatcc gcttccatat cggggatgaa atcgccataa
     1801 ctgctgtcgc cgtcgtcacc gacaggcgtt tccaggctga tagcttcctg tgagattttc
     1861 aggacggcac gcactttatc ggcactcatg cccatttcgg cgccgatttc ttcggcagtt
     1921 ggttcacgtc ctaatttttg caggaggttg cggcttgtcc gcatcagttt attaatggat
     1981 tcgatcatat gtaccgggat ccggatggtg cgcgcctgat ccgcaatgga gcgtgtaatt
     2041 gcctgacgga tccaccaggt ggcataggtg ctgaatttgt atccccgctg gtattcgaat
     2101 ttatcaacag ctttcatgag tcctatattg ccttcctgaa tcagatcaag aaaggacatg
     2161 ccacggttga tgtatttttt tccaatgctg actaccagac gcaggtttgc ctggaccagc
     2221 tccattttgg cttcgtagat gttatcttct ttttcaatga taacagcgat gagctcgtta
     2281 atttttccct tatccagccg ggcgccttcc tgaatctgat tgatttttct ctgagcaatg
     2341 agaagacgcc gttcaagttc aagaaagttg tcacgatcca gagcgtgttt tttgaagctg
     2401 ctttgattgc gtctgctttg cgtgcccagt ttcaggattt cttctgcact catgccgatt
     2461 tcgcgttcaa tgcgatccac ttcatcaaaa gcattttcga gccggcgttt gaggcttttg
     2521 attttcctgc ctattttcag gatttcacgg tttttcaatt gaaaactgcg gatgatgtcg
     2581 acctgctgct ttctgaactc ccggatacgg tcctcaatgt tctgaatact tttgggagag
     2641 agcccggcgc ggcgcattct tttttcctgc ttttcaattt cgacatccag cgcgcccatc
     2701 ttttccatgt actgtggcag ctctttgaca aatttatgat gaaattttgt gtctgtattt
     2761 tcgcagatgt gacagaattc cagacggcct gccagaatac gtgctgcaag catctgtgtc
     2821 tctttaatgg taaagggcgc atcaaggata acgcttatca gttcattttc cgctgcttcg
     2881 atccgtttgg caatttcaat ttcctggtct ttgttgagaa ggggtacccg tcccatttca
     2941 cgcaggtaca tgcggacagg gtcgtcggca cggtcaagac tgcgttgtgt cgccagcctc
     3001 cgctgttcaa ggagtcgcgc ttcttccgcc agtttgtcct gctttttttc ctgcaaagcc
     3061 ttggggttga gttcttccac aacagtaatg ccggtctgca tcagtttgat ggtcaggtca
     3121 tccatcagca tcgcatcggc atcatcgggg aggaggagca tcatttcgtt gtacgtgagt
     3181 ttcccccttg cctgagcatt cttcagtatc ctgctggatt tcgggttcgt catgatttca
     3241 tcaattttag tttctttgct cacgtgttgt tgcctttacc ggttaactga ctttatcgac
     3301 agatcctgat ttcggagact gtgtgttcag gaagtcgcca gactttccat aaactgtttt
     3361 aattccacaa tacgcttata cagttcagca gaacgcgctg catcctgatc ctgtgttgcc
     3421 cgacgcactt cctgctgcag cctgttcatt ttctgggcgc aggattcaaa agcaaaccgc
     3481 gccagtcgtt cttcgatcag ccgttccgct ttctcgggcg gcggtgcttc cgccacagca
     3541 gcagcggaaa agagattgcg ctcctcgcct tccggcaagt cctgcacgtt tattttttct
     3601 tctttccggg agtttatgag ccgggcgagg ctcagaaccg ggtgggacag ctcaagttct
     3661 gtgtctaaag cggccgcttt tattcttaaa gaagggtatt ccagcaaggc tgccagaaaa
     3721 tcaatatcat cttttgttgt cttaagcgtt tcttcgttac ttttctcatt aaagtcggcg
     3781 cgctgctcgg ggaaccgccg gcttttgata aaccgttcaa agttcctcct gcattcccag
     3841 acatggatat tcagtgcctc ggcgacaagg tttaagtact gttccagtcg tataggctca
     3901 tcaataacgc gcaaaatgct gaataaggtg tgcgccagtt cggtgcgccc ttctatcgtg
     3961 tttttctgct cgccctgcat ggctatatag aaactgacaa agtccggtgc agtatttatc
     4021 aggttccgga aagcgtctgc accgtgatcc cgaatgaaat cgtcgggatc ctgtccttcc
     4081 ggaggtgccg cagcatgcac ggccagccct gcgcctgtga ggagtgcaat acttctgagt
     4141 gcagctcgaa cacctgccgt atcgccgtca tagaccacca ccaccttggg agcatagcgc
     4201 ttgagcagca tggcctgctg ctcggtgagg gccgtgccac aggttgcaac gacattttcg
     4261 atgccatttt cggcgcagcg catcaggtca aaatagccct cgaccagaat agcggtcttt
     4321 tccctagtaa ttgcctcccg cgcctcaaag agaccgtaga gggtacggct ttttttatat
     4381 acactcgttt caggcgaatt gatatattta gcggggctgt cgcccagatc gcgaccgccg
     4441 aaggctaccg catttccgga aatatcgcga atggggatga tcagtctgtt tctgaaaaaa
     4501 tcatagatat tcttttcgcc ctgtcgaacc agtccggaag caatgagttc tccttctcga
     4561 aatccttctt tcagggcggc atccttaaaa gtcgaaaaac tgtccactgc cagcccaaga
     4621 gaaaaatgcg cttctgtcgc cgggtgcatg cccctctttt tcaggtactc ctgcgccgca
     4681 gtaccggtct cggggctttt cagctgctgg cggtaaaaac gaagggcaaa ggaattgagt
     4741 tccaggatcc tgcgccgctg ataggcctcc cggtcatcct cctttccgga cgcctccaga
     4801 tgaataccgg cacgcgcggc aagtttttcc aatgcctcgt taaaactgat cccttcaaac
     4861 tgcatgagaa attgcagcag gtcgcctccc tggctgctgc tgaagcagta aaacatctgc
     4921 ctgtccctgc tgaccatgaa ggaaggcgtc ttttcatttg tgaaggggct taagccttta
     4981 aagcgtgagg caccggcatt tttcagttca acgctttccc caatgacgtc cacgatatca
     5041 ttggcggcga gaacttgcgc aattgtttcc cgggagtatt ttttcaaaca gcacgcctca
     5101 tatgaacaga caacgccaca gaaactgtaa tttcaggcac acctctagca ctatttgtgt
     5161 caatatgata gcagaaaaaa taccgtttgt caagtgtaat ttcagggggg aatacagaac
     5221 gaagatcagt cgttttccga gggctcatcc ttgtgcagga atttgggatt attgcctgca
     5281 atgaccactg tgatttctcc ttttggatga ggggccccgt caaagcgagc atacagttca
     5341 gagagagggc cgcgaagaac ccgttcatga tgcttggtaa gctcgaagca tacagcggct
     5401 tcccgatcgc ccagcacttc cagcgcatca cggagcagta cggcaatccg gaaaggggac
     5461 tcaaagaata tcagtgtatg gggcgcatcc ttttccattt ccagaaagcg ttttcttttg
     5521 ccttccttgc gtggcgggaa gcctttgaag gtgaaggagg cgccggacag accggagagt
     5581 acaagggcag tcagaacggc gctgggaccg ggcacaagtt caacggcgaa ccctgcttcg
     5641 cggcagagat taacggcatc gtacccgggg tcactgatac agggcatgcc gctgtctgtg
     5701 cataatgcca cagaacgccc ctctctgagc agcccgataa tgcgctttgc cgcctgatcc
     5761 tcattatgtt catgagagga aaagcgaatg gcgggggagg ggatttggaa gcgctcgtat
     5821 atttttcgtg tgatgcgggt gtcttcgcag gcgatggcat ccacttcgcc cagaatgcgg
     5881 acagcgcggt agctcaggtc ttccagattg ccgatggggg ttccgatcac atagagggtg
     5941 ctcatgactg ttcgggctca atttgcggtt tgaaaatggc aatcccttct ttctgaaaag
     6001 cctccgccag tgcttgatac atggcttcgt cttcgtagat gccaatgatg gcgcctccgg
     6061 atccggccag ttttgcatga gcgcccagtg ctttcgcaca ggtaatcatg tccttatttc
     6121 gctgatccac cttgaagata gaacagcgca gctcaaaatt cctgtcaaag agcggtccta
     6181 tttccgtgcc ctttcccgca agaagcagat cccgtgcctg gcgggcgcat tcacccagtg
     6241 cttccatggt ctccagaacc tgcgcatcac cggcaagcca gcggtcacgc acggtctgat
     6301 gtattgtttc ggagccttct cccagatctt ctctgtaggc aataaaaaga gggggcagca
     6361 acgccggatt cagtttttca taattgccgt gtccctgcgc ctccatgagc gcccgctgaa
     6421 aatccatgta cacgacaccc tcgtagacct gaataacacg atcctgcaga ccggcggcaa
     6481 tgcccagctc ctcggtctcc acgctgagga ttaatccggg aagcagcggt ttggggatgt
     6541 caatatcgta aaactccatg agacagcgaa tggtggcggt tacgagtgcg ctggatccgg
     6601 caaggccgag gcggcgggga atggtggagc ggtaacggat ggaaaaatta cggtcgggaa
     6661 gcggcatgtt ctgatcccgg caatagagcc agaagcgcct gatggcagcc ttcatcagcc
     6721 ggatgccacc gtaatagcca ttgttttcca cgtcccggga gagttcctgc atgctggaat
     6781 acttcatgcg gtcctgaaaa ctgggcacca cttctatgtc gggattttca taaagcgtta
     6841 ctttggcggc gaaattgcgg agaatgaggc tgatggtttt tccgtaatat ccatccgacg
     6901 ggttgcctac aagacccgct cgtgcgtatg ctgtggattc acatgccatg gtgcgccttt
     6961 aatgagtaca cattcagtat ctgcacttgt ccggcggaca agcgcatttc agaaagtccc
     7021 ggattccttg tctctgtgaa aaggcggtta cttgttaatc aactgggaca tctgcaggat
     7081 aggcatgaag agggctacga caataccgcc cacgacggtg cccataaaac cgatgagaat
     7141 gggctcgatg aggctggtaa gtccgtccac ggtggctttt acttcggaat cgtagaattc
     7201 cgaaattttc atcagcatcc cttccagggc accggttttc tcaccgacac cgatcatacg
     7261 ggtaaccatg gcgggaaata caccggagcg ggtcagcggg tctgccagtg tttcaccgtt
     7321 gcgcacgcat tcaccggctt cacgcactgc tttggcgtag acttcgttgc cggcggtgcg
     7381 ttccacgatt tccatggcct gcagaatagc aacaccgctt tttgtaaggg tgctcagggt
     7441 acgcgccaca cggctgatgg cgactttgcg cagcaggccg ccgaatactg gaagccgcag
     7501 cttcagattg tccagattat agcggcctcc gggagtggca ccataaatcc gcaccgccgt
     7561 aacgataccg actatagcac cgatgatcat cagaatggaa taccattggc gcaggaagtt
     7621 actggtagtg ataaggatct gggtcggtgc gggcagctcg gctccgaaac ttccgaagat
     7681 ttccgcaaac tggggcacca caaaaataat aagaccggtg gcgataagaa taatcatgga
     7741 aaaagcgaca acggggtagg tcatagcgga ccggatacgc cgtttcagtt cctcactggc
     7801 ttccaggtag tccgccagtt ggagaagcac gccgtcgagg tcaccgctcg cttcaccggc
     7861 acgaaccata ctggtataca ggtcgggaaa agcccgggga aatttgcgga gcgcgtccga
     7921 aaaggtttcc cctgcttcca cttcctgggc tatggagtcg ataatcgcac tgaaccgggc
     7981 gtcctctgtt tgttccgcaa gaatatccag accctgcatg agcggcagac cggaactcac
     8041 aatagtggca agctgacggg tgaagatcag cagttcgtgg cttttgacct tgcggttcag
     8101 ctttgatgcg gtccggccga cgtttgattg cccggcaacc agacgatcgg gttcgattcc
     8161 catggcgtgg agctgacgta cggcatcctc acgatcttcc gccacgatgg aaccggtgcg
     8221 tttcattcct gactgaaggc gtcctgtgta ctgaaatgtc ggcatattcg atagtctccg
     8281 gagcttaaaa atccagtgaa accaagcgct ttttaaaaag tataattcat cagcaaacgg
     8341 aaagcaaacg gatgaaaggg acgatatggt ataatccaca gtgacaggcg ttttgttgca
     8401 ttgaaagaaa tacaagagtt gctttacgcg ggcgataatg cctgaaatat ggaaaggaaa
     8461 taaaaatggc agacatgttc ctttatattt ggacaatcgt ttttatggcg atgctctcct
     8521 acgttctggg cgactgggtc tggtatcttt tccctttcgt ctgaaatagc cgggttcgga
     8581 tacgggcgtt aaaactgctg gaaggaagag gaactatggc acaggtgcgg gtgtgtgcac
     8641 ttgcggtgtt ttttgtgtcg ctggcggcgt acggagtgga ttgtccccag tttcggggac
     8701 tggacagaac aggggtctat cccgacacgg ggctgctgca gcgctggccg gaaggcggac
     8761 cgcctcttct ctgggaatct tcaggattcg gtgtcggatt tacctccccc cttattgtaa
     8821 aagaccgtat ttacgttacc ggaacccttg ccgatcagga aagttttatt tttgtgctgg
     8881 atcaggcggg gaaggagatt gaacgcatcg gatacggcat tgaaacgacc gcggaatcag
     8941 cacccgggtc ccggtgtacg cccacgattg acggcagcga tctttttatt ctgtccgggc
     9001 ccggcgtgct cagttgcatc gatctttccg cttcttctgt ccgctggcag gtgaatattc
     9061 tcgaaaaatt caacggcccg caaattgaat ggcatctctc cgagtctcct ctggttgacg
     9121 gcgaccgggt gatctgtacg cccggcggag ccgatgctgc catggtggcg ctggaccgga
     9181 agacaggcga tacggtttgg gtaagccatg gtcttaccga tatgacctct catgtggtgc
     9241 ctctgctggt ggagcacaac ggtcgtcgtc tggtttttac ggaaacagcg aaatatctga
     9301 tctgtgtgga tgcggaaaag ggcacgctgc tatggaagcg tgaacacgag acggaatggg
     9361 atatccatgc cgtgacaccc atttaccgaa atgggcagct ctattatgtt gcaggctaca
     9421 aatccggcgg agggctcctt gaattatcgg aagacgggag cagctacacg gtgaaatggc
     9481 tggacaccga actggactgt cagcatcatg gtgtggtcta tctcgacgga tttctgtacg
     9541 gcacttcgca tcaccgcggc ggcggcaggc tggtctgcct ccgctgggac acgggtgaaa
     9601 tgatgtgggc ggacaaggcg gtgcgccagt cttccattat tgcggcagag ggtatgcttt
     9661 acgcctatga agattcccgg cgcggcacaa tgcgtctcat caaggcatcg cctgaagggc
     9721 tcgaggtgag aggcgaattc agcatgcctg gcgggccgaa agagcactgg gctcatccgg
     9781 taatagccaa cggtcttctt tatatgcgcc atggtgacac gctgcgctgc tatgacattc
     9841 gggagagccg gtaagacgta cgtgcgtttt attcctcgtc gtcgtcttct tcttcttcca
     9901 ttggtgcgcc cagaggcgta atggcggcag atgttaaatc catttgttcc ggtcccagaa
     9961 ttccgccgga aacgatgatc ttgacggcct cgtcaatcgt cagatcgcat ttgtaaacat
    10021 cttccggagc caccagcaga taaagaccga cggttatgtt gggcgtggtc ggcacaaaga
    10081 cattgtaata ttcgcgcccg tcccggcagc ggatggttcc cagcgccagc cctatacatt
    10141 tcattccggg gcagggaaat tcaataagca cggggatttt gaacgctgtg tcttcctgtg
    10201 caaacgccag cgattcgaca acctgcttcg atgctttata aatggatttt acaaagggga
    10261 ttgtctgaat caggttttca aataaagcaa tcagtttttt gccgacgacc acgctggcaa
    10321 taagccccat aaagtaaata aaggcaaaga gcagaataga ggcgatcacc ggggaggtgt
    10381 aatcgggtac cgggtcaata tactttcgga caaaaggaga gaggcgtccg gcggtaaaag
    10441 cataaagaag tttcagcaca aatgcggtca cgcccatagg tacgacaatg agcagtccgg
    10501 aaattaacgg cttccgaaag gtgtgtgtga ttttgttttt ccggggcaca gtgcatttcg
    10561 gggcgctagt ttcagccatg acgcatatcc tcgtgccggg cttccggact gtaacccgcc
    10621 ggctccccaa tcattttggg gctgattaaa ccggcggaca tgaccgtggt gatagcgtct
    10681 tcgacagaaa ttcctgcatt ctccgcgcga tcctccggca caaattcaag ataaccgcta
    10741 gtggggttgg gcgtggtggg gataaacacg cggtagtgct ttttctcttc gccgctgaca
    10801 gtgtcacgca gaacgacggt attggtgaca aatccgaagg cttttattcc cgggcagggg
    10861 aaatcaacga gtactacgga ttgaaaactg ttggcgtcat cctgcaggga caatgattcc
    10921 accacctgtt tggatgcacc gtacactgtt ttgacgaagg gaattttact gagcagccct
    10981 tcggcaaggg ctatgatttt tttgccgatg acaatatggg caataagtcc gatgccataa
    11041 gtggcggcca ggagaatcag tatggaaacg aaggcgatgc cgtattcggg tacttttgtg
    11101 aaaagaaccc gcgtgaccgg ctccaggcgc cctgcggtaa agtcatagag gaatctgagg
    11161 ataaagacgg tgagcccgag gggcaccaca accagcaaac cggaaaggat gcgatgctgc
    11221 aatatcgcct tgaggcccgg ccgttttttc ttttttttcc gctttttctt gtttttttct
    11281 tgagacatgc gaatgttttc ctgtaggcga tgcaagcgtt attatacctg tttcagcgct
    11341 gaaaaagcag atttctgcag tggcaggctt ttcttctgtt atctgcctga tttctcctgc
    11401 ggggcatagc aggaaatttt gtataaaagc ccctgtatat gttacattta tgtctgtgga
    11461 agcattggtt tcgggctgaa aaagcagcct ccggttcttg tctgaaccaa aggaaaaggg
    11521 tctctattcc tgcgggtttc agcgtttcag gcgccccacc gggattctct gtgttgaatg
    11581 atccgttgag agacgcaggc ccgtgtctct ctcatgtacc ttgcctttgc ttccgccggt
    11641 tcggttccct attgctctta ttcagtctta ggggcattac cgcccgaagt gcgggcacag
    11701 cacaaggaaa aaacatgaca gaagaaaaac gcagtctgac ctacagtgat gccggcgtca
    11761 acattgatgc ggcaaacacg gcattatcca cggtgaaaga tctggtgcgg cgcacctata
    11821 cccagcatgt gctgcgcgac atcggcactt ttggcgccat gtttcagctg gacctgcaga
    11881 attataggga gcctgtactg gtgtccagcg ttgacggtgt gggcacaaaa ctcaaacttg
    11941 ccttcatgac gggcaaacat gacacggtag gcgtcgacct cgtttctcat tgtgtcaacg
    12001 atattcttgt tcagggtgcg aaaccgctgt ttttcctgga ctatttcgcc atgggaaaac
    12061 tggagccgga tgtcatggtg gaagttgtgc gcggtctggc aacaggctgc cgctattcgg
    12121 gctgcgccct catcggcgga gaaacggcag aaatgccgga tatgtacacg gttggcgaat
    12181 atgaccttgc cggcacgatt gtcggcattg tcgaccgttc ccgtattgtc gacggcagcg
    12241 ccatccgggc aggggatgtc attatcggat tgcccagttc gggactgcac acgaacggct
    12301 acagccttgc acgcaaaatc tgcttcgagg tggcggggct ggatgttcat gatcccatgc
    12361 ccggcacagg tcgtacggtg gcggaagccc tgatggagcc gcatatcagt tatgcgaaac
    12421 tgatgcaggt gattatgcgg actgtggacg tgcgtggtat ggcgcatatc accggtggcg
    12481 gcattaccga taatctgccc aggattcttc ccgacggagt cgcagctgaa atccggcttg
    12541 attcctggac tgttccccct gttttcagat ttctgaagga agcgggcaat gtggatgaca
    12601 aggaaatgct ccggactttc aatatggggc agggatttct ttttgtggtg cccgaagaac
    12661 atgcagcccg ggcaaaagaa acgattgaac ttgctggtca gtcctgctcg tttgcaggaa
    12721 gaattataga aggcgatggc aaagtacact actcaggaaa cctgaattat gccaacacat
    12781 cgaattgaag tagcattaag gccggaattc aaagatccgg ccggcgaagc cctgcgtgcg
    12841 caggtgcggg aagatctgaa tcttgaaatc ggcgctgtcc gggtgattga tgttttcacc
    12901 cttcacgccg ctttgactgc ggaggaactc gaacgcatcc gcatggatct cttcaccgac
    12961 cctatcatag aatactccgc cgtcaatgct tcgcttgcag aaaacttcag ttgccttata
    13021 gaagtcgggt atcttcccgg cgtgaccgat aatgccggcc gcagtgctca ggaaggaatt
    13081 gaggattctc tgggccgtaa gttggctcaa ggggaagcag tatataaaag cacgctctat
    13141 gtttttcagg ataccgatgc agaaacagca aagacgattg cctccaatct gcttgccaat
    13201 gaactgatcg agcagtatgc gatcagaacg ccggaagaaa tgaaagcgct ggcaggcaaa
    13261 tcgctcctcg ccctgccccg tgtggttgaa tatgcggtgc cggaagtgga aagcatcgat
    13321 ctaaatcttt ctgatgatgc gctgcagcaa ttaagccggg atcgcatgtt tgccctggaa
    13381 ctgggcgaaa tgcaggtgat ccgggattat tacaatcggg cagatgttca ggcacaccgc
    13441 cgcagcatcg gattgcccga taaacccacc gacattgaaa tggaaatcct tgcccagacc
    13501 tggtcggagc attgcaagca caaaatcttc aatgcagtga ttcattacga cgaaggcaac
    13561 ggtaaggttc gggtgattga ttctgttttt aaaagctaca ttaaaaaact gaccgatgag
    13621 gttggcaagc gggtgccctg gctggtcagc gtgttcgatg acaatgcggg gattattcag
    13681 tttgatgaaa atcacctgtt cgctctgaaa gcggaaacgc ataacagccc ctcggcgctg
    13741 gatccctacg gcggcgccat gaccggtatc gtcggtgtga accgtgacgt gctgggtgcc
    13801 ggtctgggat ttcagtgtat ttttaatacc gatgttctgt gcgttgcatc gcccttccac
    13861 gatgaagaaa tcccgccgcg gctgttgcat ccccgtcgta ttttacgggg tgcccatgcc
    13921 ggagcccggg atggcggcaa tcagagcggc gtgccgaatg tgaatggcgc gctggttttc
    13981 catgagcgtt ttcttgggaa gccgctggtc ttctgcggaa caggcggact cagcccggcg
    14041 tatattcagg gacggccggc acataaaaaa gcggcgcgtc ccggcgatgc tattgtcatg
    14101 acgggcggtc gaatcggaaa agacggcatt cacggcgcca cgttttcatc agaagccctg
    14161 catgaagggt ctcccgcaac ggcggtgcag atcggtgatc ctatcaccca gaagaaaatg
    14221 tccgattttc tgatggaagc ccgggacgcc ggtctgtata actgtattac cgataatggc
    14281 gccggcggcc tgtcttccag tgtgggcgaa atggcggaga agccgggcgg cgccgtcatc
    14341 catctggaca aagcgccgtt gaagtatgcc ggtctggcac cctgggaaat ttttgtgtcc
    14401 gaagcgcagg agcggatgac gctggcggtt cctcaggaga cgctcgggcg ttttctggat
    14461 ctggcagagc gccggggcgt tgaagcgact gtgctcggcg cgtttaatga ttccggatat
    14521 ctggagtgct tctataaagg ggagcaggtt gcttctcttg atatggattt tctgcatcac
    14581 ggtctgcccc gcatggaact ggaagcctgc ctggcaccgt cgcctgatcc tgtggaagtg
    14641 acggcgggcg aaggcaaatc ggatctgacc gatgaccttc tgtcgctcct cggctcattg
    14701 aatatctgca gcaaagaaaa ctttgtccgc atgtttgatc atgaagtgct gggacaaagc
    14761 gttttaaaac agtttcaggg agtgcagcat gacgggcccg gtgatgccgg tgtgatcaga
    14821 ccgcttcccg acagttaccg cggcatagca gtttcctgtg gcatcagccc gaagtatgct
    14881 gatctggatg cctggtccat gacggcgtgt gcggtggatg aggcagtgcg taacctcgtg
    14941 gcggtcggtg ctgccatggg caccatcgcg gggctggaca atttctgctg gcccgatccg
    15001 gtacagagcg acagcacacc cgacggcaaa cataaactcg cgcagctgat ccgctcctgc
    15061 gaggcgcttt acgaaatctg cctcacctac gatattccgc tgatcagcgg caaagacagc
    15121 atgaaaaatg actaccgcat cggtgacacg aaaatcagca ttccgccgac gctgctcttc
    15181 accgcagcag ccattattga agatacgcga cgtgtcgtga gcgtggatgt gaaacaggca
    15241 ggcgaccggg tctatgtact gggcgatacg ctgaatgaga tgggcggaag cgagtacttc
    15301 acgctgcaac agatatccgg cgggaaggcg ccgacggtgg atccgaaaaa agcgcggacc
    15361 ctctatgaag cgctgaaccg cgctattcag ggcggacttg tggcatcctg ccacgactgc
    15421 tccgacggcg ggcttgcggt ggcgctggca gaaagtgctt ttgccgggaa tctcggcatg
    15481 gatctatcgc tggaggctct gggtgtggaa gacggcgtga cagccctttt cagtgagtcg
    15541 caaagccgtt tcgttgtgac cgtatctccc gaccatgccg aagcttttga agcggttctt
    15601 gcgggcagcc cctgttactg tgtcggtgtg gtgacggaag aacctctgtt ccaaatcaac
    15661 tgtccggacg ggcggaaaat cagtgcatct ctggatgtgc tccgcagtgc ctggaaaaaa
    15721 actttgaact ggtagaaagg aagatccgtc catgacagtt aaaactcttg tacttaccgg
    15781 tttcggtatc aactgcgacc aggaaaccca tcaggctttt gcttgtgccg gttcagacgt
    15841 gacgcgcgtg catttgagtg accttctcga gactccggcg ctgcttgacg gtgcgcatat
    15901 ttttgttgtg cccggcggct tttcctacgg cgatcatgtg tcttcgggga aaatccttgc
    15961 caacaggctt cgccaccagc tcgaggtgcc gctcaggcag tttgtggaaa aagggaagct
    16021 cattctgggt atctgcaatg gatttcaggt gctcgcaaaa atgggaatac tgcctttttt
    16081 cgatggcacc gtatctcagg aagttacact gacaaccaat gaaagcggtc gctttgaaaa
    16141 ccgctgggta catctggcag ttcctgaaaa tacgccctgt atctggctga aggggattac
    16201 aaagtttgag ctgccggtca ggcagggcga aggaaagctg atcgtccgca atgatgcggt
    16261 acgggatcgt ctgcacaagg aaggccttgt agcgctgcgc tatgtccgtc ccgacggagc
    16321 gcctgccgca ggtgtgtttc ctttcaatcc cagcggcgct gaagatgata ttgcggcgtt
    16381 gtgcgatcca tccggtcgca ttttcgggct catgccccat cccgaagcct ttctcgacgg
    16441 gatgaatcat ccagaatgga cccgtctcgg cctggcagaa gagggcgaag ggctggcgct
    16501 tttcagaaat gccgtggagt ttgcccgcac atccctatgc tgaagctgcc gcaagacgct
    16561 aaaggcacag gatatgtctc atctacaccg ttttttttcg tctcttccgg aggaagggaa
    16621 agcatctttt ctccttcccc ctgaagaggc gcatcacgct cttcgtgttg tccggctgcg
    16681 ggatggcgac acggtttctg tttttgacgg ccggggcggt gaagtggaag ggcggctgcg
    16741 gccgtgcggc aaaaaagaag cggaaatcga aattgtccgg cggatgcagc atccggagcc
    16801 tgctgtgaag atgaccgttg ctgttgcctg gctgcacagg gataaagcac atgaagatat
    16861 tgtccggcgt gcggtggagc tcggcatgtg gcgcattatt ttctggcgtg ccgatcattc
    16921 gcagaagaaa tgtgctgctg ccgaccgatg ggaaaaaata gcgctggaag catgcaagca
    16981 gagcggcagg gtctttattc cggaaattga acttcaggat tctctggatg cgctgtttga
    17041 aacagtatcg cgcccggtga ttcatgccct tcctgcggcg gatcctttgc agaagcccga
    17101 aatcctggtg catgatcagg tgaccatgct catcggcccg gaaggcgatt tctctgatag
    17161 ggaagtggca ctggctcagg aacagggcgc tattcagatt cacatgggaa actgcatttt
    17221 taaaagcgaa gttgccgctg ctgttgccat gacgctcctg gcggctcagg tgggggagct
    17281 gggacgtcat ttctcgattg tttccggata agccctgccc gggagatctt atgaaaattc
    17341 tttttctctg ccagtatttc ccgcccgaac cgggagcacc ggctgcgcgg acgcatgagc
    17401 acgcgcggga atgggtacgg caggggcatg atgtgacggt gatctgcggc attcccaatc
    17461 atccggacgg gcaggtgccc cgttcctatg ctcatgaata tgtttatgac gagctgtatg
    17521 atgatatacg ggtgcttcgc tgctggtttt ttgtcacgcc caaccggggg cgtatacggc
    17581 gcagcctatc ttttgcaagt tttatgctgg cagcactttt ctgggggatt gtccggggcg
    17641 gcagggccga tgttgttgct gccacatcgc cccagctgct ttgcggcgct gccggctatc
    17701 ttctctcgct ttttaaaaga tgcccctttg tttttgaagt gcgtgatctg tggccggatc
    17761 agattgttga tctggggctg atacgcaata cagccgttat caaagggctg atgaaactgg
    17821 aagcttttct ttaccggaag gcggcagcca ttgtaactgt tgcggaggca acacgccgga
    17881 atctcatcgg gcgcggcatc ccgccggaca aagttcacac cattaccaat gccattgcgc
    17941 tggatatttt tacaccgctg ccccgggtgg gcgtcatgcg gcaccgctac ggatgggatg
    18001 acacctttct ggtcatgtat atcggaaccc acggtatttc acaggggctc gaaaccgtgc
    18061 tggaagcggc agagaagctg cagagcgctt cagggcttcg ttttgttttt gtcggatccg
    18121 gtgccgaacg gatgcggctc aaggaaaggg cggaaaaact ggggctgacc aatgtggaat
    18181 ttattccgag ccaaccccgg ggtgacatgc cggaatttta tgccgccgct gacctctgtc
    18241 tggttcctct gaaaaaaaga aatgtttttc tcaccaatat ccccagcaaa atgtttgaga
    18301 ttatggcgtg tgcccgcccc attctcctgg gagcggaagg tcaggcgaag gaagtacttg
    18361 aacaggcggg agcaggtgtt gcctttgcgc cagaagatgc agacgctctg gcagccgcga
    18421 tttgtgcgct gaaagatgat ccggagaagt gccggaggtt cggagaatcc ggacgccact
    18481 atgtggagca gcactgcaac cgtgctttga aagcaaagga aatgcttgct gtcctggaag
    18541 aagtgcagca gcgtaaataa agcgcatcca tcattgacgg gagctgtttt ccggtttttt
    18601 tagcaaattg ttttttttgt tttgtgttct ttcttctgcg tacactatag aatattcagt
    18661 agagtgtgtt ttatcttttt caaaggaggg tatgtattat ggcacgtttc cggtttcttc
    18721 cgtgtctgga ttccgatgag atggcggcgt cccgcaaact gacttcatgg atagatcgag
    18781 atctggcgag gagcctcggg gagcgggcag tggtggcggc agaagatgga ttttattttc
    18841 tggaagacgg cagcaaggta gcctggcgta cacaggtgga gagagcgcgt gcgtcccgca
    18901 tcagcattgc cccggaggcg ccgctgcctt tgcatgtggt ggagcccgtc gaggaaacag
    18961 tgattcaggt cgccaataaa acgacgctgc aggcaggccg ggagcttgtg gatgcggggc
    19021 atcgcccgct ggcgcttaat tttgcaaacg gtatcacccc gggcggcggg tttctttcag
    19081 gagcacgggc acaggaagaa gggttgtgcc ggtcaagtgc tctttttgtc actttgcaaa
    19141 atgatgccat gtaccaattt cacagggcac ggccccgccc cgattccgcg gagtggtgta
    19201 ttgtctctcc cgatgtgcct gttttccggg atgatgcagg caatgcgctt aaggaaacct
    19261 ggctgctgag ctttattacg tcagcagcgc cttatgcacc ttccatcgga cagcctgaat
    19321 cgggagatct gctgaaaaaa aggattcgtc gggtacttgc catagcgtct gcttacgggt
    19381 ataaagcgct tgtgctgggt gcctggggct gcggcgcttt tggcaatgat ccgcagcgta
    19441 cagctgaaga ctttcatgag attttgacga cggaattttg cgggcacttt tcgaatatcg
    19501 tttttgccat taccgactgg tcgccggagc ggaggatgct cggacctttc cgtgatgtct
    19561 ttcaatgaac ggaaccgggc tatgccctgt gttgcattgc cgggagtgcg ttaaactgat
    19621 tttcgcctga tcgccgtatt ttcgccgcct tctctttcag attttgttgt gccttcattg
    19681 ctttttctta tactatacac agagtctgaa ttttctacta ttgaaccctt tatggagagt
    19741 gctttcatgc tgactgtctg gcttaccctt gctctttgcg ggcagcttcc cggcgaaaca
    19801 atgtattacg ccgaagatgc ggaacatgtt catgtttatt atgaaaaggg gcgtttcggc
    19861 ggatggcctg ccaatcacgg catgtggtgc tggggcaatg agatcctggt tggcttcagc
    19921 cgcgggtatt acaaggatct tggcccgacc atgcatcata ttgaccggga aaaacccgaa
    19981 gaattctggc ttgcccggag tcttgacggc gggcagagct ggagctggga gcatccggcg
    20041 gagaagggat tcctgatccc gcgaggaaag gcgttgcacg ggacggaaac gcccggactg
    20101 tctttgccgc ccctgcgtga agtggagacg cccattaatt ttcagcatcc cgattttgcg
    20161 ctgacattcc gtatggacaa catagacggc ggtacgtcac gctttgagta ttcctacgac
    20221 cggggcagaa cctgggaagg tcctttccgt ctgccagatt tcggcacgtc cggcactgcc
    20281 gcccggacgg actacattgt gaacggcgaa aaagatgcct ttatttttat tacggtctcg
    20341 aaatccaata agcaggaagg acgcccgctc tgtgcgcgta cccaggacgg cggcattacc
    20401 tggaaaatgg tttcctggat aggttccgag ccgaaaggtt ttgccattat gccgtccacg
    20461 cttcgtatct ccgacacgga actttttaca acgctccgct gccgggaagg tacgttccga
    20521 tggatcagtg cctggcgcag tccggataat ggatatacct gggtacagga agaaaatccg
    20581 gtggattatc tgggcgaagg caacccgccc atgctcaaca aactgcagaa cggcaggcta
    20641 tgccttacct acggatttcg tgcttatccc ttcagtatcc gtgcacggct gagcgaagat
    20701 aacggaaaaa cctggagccc tcagatagtg ctgcgggatg acggtttgga ccgtgatatc
    20761 ggctatgtcc gcagcgtcca gcgttccgat gggaaaatgg tgacagccta ctatataagc
    20821 gaaaagagca ccggtccgga gcgttatatt ggcgctacga tctgggatcc caaccgcatc
    20881 agccctatca ccagcggtga ccagaagagg caggcagggt tttaattttt caccgcgaga
    20941 aaaagattct gtgcggcgca ttgttgaata agtatccggt gttgtgcctc ttcgttctca
    21001 gactccatcc tttcgtaaaa cgacccgttt ttttgtttca ggagtatttg tccaatgaat
    21061 gatcagttgt ctcaaactga ggatcaggaa atcatccaat cggaagccaa gccgccggca
    21121 gcatctgccc gtattatgag tgtggatgtg ctcagaggct ttgatatgtt ctggcttgtt
    21181 ggcggtgccg gagtggcatt aggcatagga aaacttatag accccaaagt tcacgcgttc
    21241 atagaccgtc agctgtccca cgcagactgg gaaggctttt atttttatga tctgattttt
    21301 ccgctttttg tttttctggc gggcatgtca ctttcttttt ccatgggacg aacgctcaat
    21361 gagaaagggc tcggggcaac gctgattcgc cttacccgcc gctgcctgct gatgtttctt
    21421 ctgggtgtga tctacaacgg cggctttaca aacgtctggc cggaagtgcg gatcatgggt
    21481 gtactgcagc ggatcgcctt gagcagcctc tttgcggggg ttctattctg cttcttcgac
    21541 tggcgcggtc tggcagcatc gctggcagcc ctgctcctgg gttattgggc gcttttgagc
    21601 tttgtgccgc ttccgggtca ggacagtatc tcctgggctg tggaacagaa ctgggcggtc
    21661 tggctggacg cacagattct tccggggcgg ctgatttaca aaatttatga cccggaagga
    21721 atcctgagta cgatacctgc catcggcacc tgtatcatcg gtattctgac gggtcttttt
    21781 ctcaagagaa aagaggtcga gcctttgcgc aaagggctca tatttatcgg agccgggcta
    21841 gtgctggtcg ccgccggata tttgtggtct tatcagtgtc ccattattaa aaaaatctgg
    21901 accccctcct atgtacttgt agcgggaggc tggagtatcc tgcttctcgc cctgttttat
    21961 tttctggtcg atttctggca aatccggtgg tggactgctc cctttctctg gatcggcgtg
    22021 aacccgctta ctgtttacat ggcaagaaat atcataggat ttaatgacct tgccaaacgt
    22081 tttgtcggcg gcagcattta cgcatttctg ggtgaaaatc tcggctattt tgtgcagtgc
    22141 agcgtgtcct tgggcattgc acttcttttt gtctggtatc tgaacaagaa aaaaatatac
    22201 ctgcgtctgt aacagcaggg tcgattattc cggagaattc tgacatgacg ggacgtactg
    22261 ttctgtatgc cgcagtgctg tggcagctgt tttgcacctc agtttttgca gcggcacccc
    22321 ttcaggaacg tttcgccaat cctccggcag atacacggat tctgcgtatt attcatcatc
    22381 tgccggcgac agaagagctg cgggcacatc tgtacaaccg cttcgccgcc atgggtttcg
    22441 gaggcatggc gacgaatgtc tcttttgatt cctatcttgc agatgaagag aaatggacgg
    22501 ctttcaccga aggagtgaaa gaggcaaagg cgcgcgattt ttcgctctgg ctttacgatg
    22561 aatgcggcta cccttcgggt gcggcaggcg gtatcacgct ggagggacat ccggagtacg
    22621 aagcacgggg gcttcacatt tacgaaacgg ccggggctga aaaagacccg acagtgcctg
    22681 tgccggcggg aagggttgtt tatgtgctgg gtattccgga tgaaaccgca gttgttgatc
    22741 tggcggccgt cacggacctg agcggattgg ttgcagaggg tcagctgcgc tgggcagatg
    22801 cgccggatcg ctggcgtatt cttttctgta tggaagatga actgtatgag aatacacatg
    22861 cggcaataag tcttgccttc aaactgcgct acattaattt gctttgccct gaagcgacgc
    22921 gccgctttct tgaagtgacc catgatgcct atgcggcgcg tctggacaac aggctgcagg
    22981 attattttgt atctaccttt accgatgagc cgtcgctcat gagcctcttc atgaaacgct
    23041 ccgatcatcg cgtgctgccc tggtcggatg cactcccgca gctttttgaa agccgatggg
    23101 gctactcatt gaaagaagcg ctgccgtctc ttctttctga taccaatggc agggacggca
    23161 aagtgcgcca tgatttctgg aaaaccattg gtgacctggt ctcggaaaac tacttcgggc
    23221 agataaggcg gtggtgtgat gatcacggga ttctcagcgg cggtcacctg ctttgcgaag
    23281 aggattttct ggcggcgatt cctttctacg gcaatttttt ccaatgcgcc cgggaactga
    23341 cggctccgag tattgactgc ctgaccagta ttcccgatga agtgccctgg tactccgcac
    23401 ggctgctgag cagtgtggcg gaactcgata accggcagat taccatgtcg gaaacatccg
    23461 atcactctca gcgctacaga acggaagagg attcacgtcc tgtttatcat gtaagcgaag
    23521 aagaaatccg gggcacgctg aacctgcttc tgttaaacgg catcaataca attaccagtt
    23581 attatgtctt caaagatctt tccgacgaag cgttgaaccg gctgaatgaa tggaccggcc
    23641 gatgcagtac attgctccgt gacagcaggc aggtgaccga tattgcgctg gtttatccgg
    23701 tggaaagcgc ctgggcgcgt tttactccgg cccgtttctg gacagctgat tctcctgcgg
    23761 cggcgcatca tgttgcaaaa tgcttccatg atgcggaaga aaatctgttt tgtgcccggc
    23821 gggacttcat tcatgtggat aatgaaacgc tggaacaatg ccgtgtcgaa tccggtgtgc
    23881 tggcgtatga tgccctttgc tggcgtgttg tgattctgcc cgatgccgat acgttgtccc
    23941 gtgcggcgtg gcagaacctg tatcgttttt atgaagaggg cggtgtgttg attgccctca
    24001 gttccagacc tgaaaacagc acggagcatt tccctgatga ggatgtgcgg tctctgggca
    24061 gggtgatttt cggggaagaa aacagaccct gtgtacggat tcatgaaaaa ggaggcattg
    24121 gaatttttct tccgcccgga tcggaagcgc tgctgcccga ggtattggac aggctgatac
    24181 cgccggagat caaattgagc gaaggtgaaa acttgcgggt gacccatcgc aggctgcccg
    24241 attgcgacct cttttttatc ataaacaaca gcagcgaagc ctggaaaggc gaagctgttc
    24301 tgccggtgac tggtgccgta gacgcctggg atccggcaac aggttcacgt accagatttg
    24361 ccgcaggaaa gccggtttcg cttgtgttgg acggctacag cggaatcttt cttctttgcg
    24421 aaccgtgttc ggtgccggag cgttcccctg tgaaggaggg cgtacttccg gatttcgtat
    24481 gtgagcctct gccggaagtg tctcctgcca ctacagcagg ggaagatgtt gacgtgtcct
    24541 tgcagagggt tatgcttccg gaagaagaag cagcaggctg gcacctggag ggcagactca
    24601 ctgcggatcg ggtgaatact tttcttttcg ccggttttgt gcatgagcct cctgtggatc
    24661 ttacggacag cgtgtcgctc tgctttacgg cggctcttcc ggaaaagcag caggcagcga
    24721 taccgcttct tgtgatcctg atcgatacgg caggcgttga atatcttgca gagaccggta
    24781 ttcccatgag tggctcaggc atccatcact gtcaggtgcc cctgcgccgc ttccgccggg
    24841 caggctggtg cagtcaggtg gacaaagcat tggatttcag ttccataaca accatacgga
    24901 ttggctgggg cggctattac ggccctgaag gtgaagtggt acgtttcagc gtgtcggcat
    24961 tggggaaaaa caggatcccg gaactctgag cttgctccga tgcttaagcc gtctgtgcag
    25021 ccttcgccag ttttcgttcg tagagcgttc cggcacgggg cacatgtgat gcgacaaaag
    25081 ccagaacgat ggcaaaaaga gcggcactga ggaagagccg ttcgtagccc agccctaccc
    25141 agatagcacc cgcaatggat gggatcacca tggaagccac atggttgatg gagacgccca
    25201 tggcaagaga agagttgagt tcctgaatgg agggggtcag ccgcgacaga taaagagagc
    25261 gggcgttacc cagtgcaaag agcatttcgt ccagaataaa gcaaccacac gccagacgga
    25321 gtgcccatcg ggggtcgggg attacccgct gcgcatagcc gtagccgagg cagataagag
    25381 caagcacaac gccttcaaga atcatgacgg ttcgctcgcc gaaataatcc attgccatac
    25441 ctgccagcgg tttgaaaaag acgccgatga ggcatgagag catgagcagc ccggcaatgg
    25501 aagcggcagg aagtccgtac accttgatga gtacccaggg cccgaacgtg agaaatatct
    25561 gcttgcgtgc accggaaaga aactccacca gatagtaaag gcgatactct ttttttataa
    25621 caagacgctg acggggggta cgcagattcg gaatattcat ggaggtgtag aaaagtgcgc
    25681 ccagaagcat ggcgcatgcg gcccaggcaa aattcctctg atactgggga gcaattttat
    25741 ccatgaaaag ccatacggag cctgtgccta tggcggtgaa aagagtctgc acggcagcag
    25801 cctgaccgag acgtgtcccc cgtttttttt cagagctcag tccgatcaca atggatgcgc
    25861 ctaccggcat cagcagatgg ttgccgatac tcatgccgag catgcccaga atcacaacag
    25921 gataggaagt gccgcttttc gaaagaagaa acagacccag cgcggaggta caggtggcga
    25981 ccagaccgac ccgggtcagc gacagcatgg caagcagccc ggtgaacatg acgaccagaa
    26041 agccgggcag ttctcttgga aattcgagat ggccccgggc atcggctgtc agtttaaagg
    26101 tgtcggcaag aaaattgttg aaagtggagt catgcattcc tgtgcccaat gccacgcacg
    26161 caagggcaca gtaatacaat acaatctgat atcgcaaagg aacggaaaac atataaggat
    26221 cctgaatcgg gcaagccggt gagtgcggat gtgaaaaaaa agcagtcacc gaaaaggcgg
    26281 gatggggaaa agcctgcttt taaattatgc tatgatcact ctttaaaact ccagaaagca
    26341 gggatgcatt cccggtttgc agcaggtgca cagcataaaa atgctttgaa ttattttttc
    26401 aaagtggtat gataaagaca tcttttgcgg gcaggaatgt aaccgtatct ccgggcttgc
    26461 tcatagcccg tcatacaact ggctgatcgg acacaaagga agggtatctg tggattcgac
    26521 cgattttatt gacgagagcc gaccgctttc ccgtgatcag cagtactggc agctgcgaat
    26581 cctgatctcc acctacttcg gttatgccgg ttattatctt gtacgcaaag tctttaccat
    26641 ctgtaaaagc actctggcgc ttccggtggc ggagggcggc tacggcatgg gctttaatgc
    26701 ggtggctaat atctggacag cgtatcttgt cgcttatatg ctcggcatgt ttgtaagcag
    26761 ttttatcgga aggaagtggg gcccccgtgt gctgctgctg ggcgggctgg cgctgtccat
    26821 gctgtgcaat gttattttcg gttttgccaa ttcatatcct gtttttgtgg ttttcatgtt
    26881 tttcaacgga ttggtgcagg cggcaggctg gccgggtacg gtcggtgctg tggcggagtg
    26941 gctccggaaa aaggagcggg gcgcgatcat gggtttctgg tctaccaatt atctggtagg
    27001 caatattctg gtgaaatcgt tgggtggttt tctgcttgct acttacagcg cccgttacag
    27061 taccgtgatc ggtgtccgtt attcttttct cggctgcacc ttgcttgctt ttgcgttctg
    27121 gtggcttctt tttttctggc agcgtaataa gcctgaggac gcagggctcc cgccgattgt
    27181 ctcctccggc gctgatgaag cacggagtgt ggaggcttcg caaagcaaaa gcgtcacttt
    27241 tcgccagtac
//
LOCUS       DAOPWB010000029        27165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1754_length_27165_cov_5.009443, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000029 DAOPWB010000000
VERSION     DAOPWB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 27165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27165
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1754_length_27165_cov_5.009443"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1518
                     /locus_tag="PKN92_04375"
     CDS             106..1518
                     /locus_tag="PKN92_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ48061.1"
                     /translation="MTFQQKQLIDETRWTGTREVLSMSGPLILGMLSYTIMEFFDKVM
                     CSYLGTDALAAVGSAGIASFTLCTLFMGIASIVTTFVAQCFGREQYTLCACYCWQGMY
                     ISVLGIFFAAVLWPLAPLLFGSMGHNETVTRYELEYFRARVLGYFFVGAVTALAGFFQ
                     SVSRPRIPMYAAIIANASNLILNYLLIFGKFGFPRMEVAGAAVATVLATILQFAIMLS
                     FFLHRHFDSRYRTRASWAFDWHRLREMITIGTPSAISMFLDVANWWIFTAFIIGRFGA
                     IQLAANTIAVSFIHICFLPAVGLNHGIAAIVGQWLGRRDVERAKRRTYTAIKIAGVYM
                     VAMGITFALFGDTLISLIFRAEPEVVHLGHKLLILAALFQAFDAVNIVCTGALKGAGD
                     THWMMYVTFVMSYLIFLPLALLFAFYFRMGAFGAWIGATVFIILLSGVLFRRFRKEGW
                     RNINIFTRTAREGTSSAQGH"
     gene            1557..2525
                     /locus_tag="PKN92_04380"
     CDS             1557..2525
                     /locus_tag="PKN92_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNZ48062.1"
                     /translation="MSSLVHLTVDDGENPVRLDVYLAEALKSFSRSHIKKLIQQGSVT
                     VNQCICTKAATVVQPGQSIAVELPEAPPLVPVPENIPIRILFEDQHLVVVDKPSGLVV
                     HPAPGHYTGTLVNALLFHCPDYEQTGDDSRRPGIVHRLDRYTSGVLVAAKNQHAYLHL
                     ARQASERHFERRYQALVAGEFPEDRGTVNASLGRSLVDPSRMAVTGVNAKTAITHFET
                     LERFGVACLVRLTLETGRTHQIRVHMRFAGHPIFGDPVYGTCNYDTPGVPEELLSALM
                     RLEGQALHAEKLGFTHPVSGEWLTFVSSPPEDFQNALKALRLFKKE"
     gene            2795..3370
                     /locus_tag="PKN92_04385"
     CDS             2795..3370
                     /locus_tag="PKN92_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ48063.1"
                     /translation="MSATSTRWSKEFEDAVMEHVDLLYAVALRLTRNHADAEDLTQNT
                     FVKALRFHDKFKPGTYIKAWLLTILRNTFINEYRRRSRRPLEVELSGLEVARGTHPDP
                     EVFFEPRPGCREDLLELVDDPVRKAVEALPEEFREAVLMADLEDMSYKEIAEAMGCPL
                     GTVMSRLYRGRKLLRDALEEYARDRGVVSPK"
     gene            3566..4645
                     /locus_tag="PKN92_04390"
     CDS             3566..4645
                     /locus_tag="PKN92_04390"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNZ48064.1"
                     /translation="MAKYSVAQIPGDGTGPEVMAEGVKVLDAAAAKFGFSFSYQNYDF
                     GGERYMKTGEVLPDSAIDEFRKFDALYLGAIGHPDVAPGILEKGILLRTRFELDQYIN
                     LRPVKLYPGVDTPIKDKGPEHIDFVVIRENSADIYTGIGGVQRKGTPYEVAMQTMVYS
                     RNEVDRCLRYAFECARKRNKNNTLTLCGKTNVLTYVFGLWERAFHEMGEKEFPDIKRE
                     YAHVDATTMWMVKNPEWFDVIVTGNMFGDIITDLGAMIQGGMGIAAGGNLNPEGVSMF
                     EPIGGSAPKYTGMNIINPLACIFAGQMMMDHLGETEAAKAIEDACVAFLTSGLIKGLA
                     ASDIKAAGMSTSAIGDWIADYIAKN"
     gene            4780..5802
                     /locus_tag="PKN92_04395"
     CDS             4780..5802
                     /locus_tag="PKN92_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNZ48065.1"
                     /translation="MLLREIIEQREIDTLSPSACFARYSRGRAMPEEEDPYRTAFQRD
                     RDRILHTKAFRRLKRKTQVFLAPEGDHFRTRLTHTLEVSQIARTISRALFLNEDLTEA
                     IALGHDLGHTPFGHAGETVLNEVYSAGFKHYEQSLRIVEHLEKRKTGAGLNLTWEVRQ
                     GILNHSHGKAILYDQPVPDLSSECLVVSLADAIAYINHDIDDALTAGVISFDALPKEA
                     VTAVGEGNSKRINSMVLGVIEGSSGGEINILPEIKRGMRILRDFLYQEVYPREAIESE
                     IRQARQMVRDLYCYLLEHPPAQMNHDDTDDSFERRLVDYIAGMTDEYAMRLHHSIFKG
                     SQTGQR"
     gene            complement(5782..6501)
                     /gene="bshB1"
                     /locus_tag="PKN92_04400"
     CDS             complement(5782..6501)
                     /gene="bshB1"
                     /locus_tag="PKN92_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNZ48066.1"
                     /translation="MTTHQESVDVLAIGAHPDDVELGCGGLLAKLSSEGKRIAILDLT
                     RGELSTRGTPNERHAESEHAAAILGLCARENAGLPDGGLSNTPEQRLAVIPFIRRFRP
                     KVVLTLMTPDRHPDHEAAHYLGRDACFLSGLMRIDTGQEPFRPERIYYFHPYLDAAAS
                     PFGIVDISGFFPKKLASIGAHKTQFYNPDDRSAGTYISSLEFWESIEHRARFWGRRIG
                     VCHGEALYSEGPVSLSTLPGL"
     gene            complement(6498..8060)
                     /gene="bshC"
                     /locus_tag="PKN92_04405"
     CDS             complement(6498..8060)
                     /gene="bshC"
                     /locus_tag="PKN92_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HNZ48067.1"
                     /translation="MNSLYHAYISGEDRVADLYEALPASLFRDRHSPAPFPPAMAEQV
                     NRYQQQLGVEKTDLPPQCRVIITGQQAGLFGGSLYTVYKTITAIQLAERVSRPDAPVI
                     PLFWTASDDHDFEEINHATLVTRRHDLLTLRYTPSPADGYVKGMPAGEIPLTGQLNKL
                     IDQAAAVTPSSECTEEIRDFLIRALGESASVAEWFSRIQARLFAHTALRLFDPRLPAA
                     RRTAAAILEQEIEAPLESTALLMRQGDILESRGFGRPIRRTETMCNFFLEIEKIRCTM
                     FYENGRFHAPEAERSFSKPEMKALLAARPELFSGNVALRPIIQQALFPVLAYVAGPGE
                     IAYWAQLKPLFNLFDQPMPIVYPRMKGLITTLKNNKLRKRFSADTTQPFDLNQVLTRA
                     LEQEHANRYHNAFQKGRISLDDAFAHFLAPFLAPGTPAAVQQLAQRFQKNQQFYFDRF
                     GKALLFSDQEKRKTVENQLTRLHCFFSPNGKEQERVLSLFSFLFEQGWSLIDRMLDVF
                     SPDKTGVQEFEL"
     gene            complement(8057..8815)
                     /locus_tag="PKN92_04410"
     CDS             complement(8057..8815)
                     /locus_tag="PKN92_04410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ48068.1"
                     /translation="MTEKKTIPEDWFESAFGDLYNIVYAHRSVASAAPEARFAAEAAG
                     LSRKDTALDLCCGTGRHLATLMDTGASLTGYDYSPVLLAKALQNTRRQVHLVRGDMRY
                     LPFTRTFSVIFSFFTSFGYFMDDGENKKALLQMSDALAGGGRFFFDYLNPEYLKQNLV
                     TESTRDSLGFRIREQRWIDEKTQRVNKTIRVEKNNVLVGKSSESVRMYTFDELRFLLE
                     QAGLRITQTWGNYDGTPYSTKTPRMLVLGIKGAA"
     gene            complement(8859..10169)
                     /locus_tag="PKN92_04415"
     CDS             complement(8859..10169)
                     /locus_tag="PKN92_04415"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNZ48069.1"
                     /translation="MERITCKFGGSSLADSSCIRQVIDIIRANPARKYIVPSAPGKRT
                     AEEKKITDLLYAWHNMARQGLDPTEPKSIIEERFSTLARELGVSFNVQPCLEEIAEHA
                     SRDEAPDYMASRGEYLNGKLIAEALGAVFVDPAECILFKENGQLDPRSYDLLGEKLQG
                     DGLFVVPGFYGATPKGEIKTFSRGGSDISGSIVARATRSSLYENWTDVSGFRMADPRI
                     VPDAKRIEEITYRELRELSYMGATVLHDEAIFPVREPGIPIQILNTKKPDSPGTLIVA
                     RRDSGHPVCGIAGRRGFTMFNIEKTLMNKEHGFGRKLLSIFEEHRVSWEHMPTGIDTI
                     SIIASDDELKGKVQTILESIERLLDPDDLTVTSGLAIVATVGKGMNRLVGVAARLTGA
                     LAEAGVNIRVINQGSSEMNILIGVEEQDLEKAVSAIYHAFDSWQ"
     gene            complement(10216..11880)
                     /gene="rseP"
                     /locus_tag="PKN92_04420"
     CDS             complement(10216..11880)
                     /gene="rseP"
                     /locus_tag="PKN92_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNZ48070.1"
                     /translation="MLINIVIFVAVLSLLIFIHELGHFLAAKSFGIYVKRFSIGMPPR
                     IFGFQWGETDYCIGALPIGGFVMMAGQEDVPLSDEEREEEYGHVPEDRWFKNKPVIQR
                     MCVLLAGPSMNFVLAVVLYALVGLVGSEVPEWEVEARVGEIEAGSPVETAPLFLLHNT
                     DAADMTGTPDAQGWQVGDRILSVNGREIGNMTDLAITAVLGGDKKTYDVLLERIDGRR
                     FVSPVVPQVLDASGHPRFGVGPFETAIVREVFPGSEAEAAGLKPQDIIRAVNDKTVSL
                     TSFITSIEKIPAGESVRLEVERNGETLALQATPETIGRIKGISFGSSEKEQELRILAI
                     ETKTAEESGLKAGDMVTAVNGKAMSPAEFFTFEKENPGSEFDLSVQRPGILFGVVEPE
                     QKINASLKIQAVGAIGVALRPKTVWHRVPVVRIIPHSFYQSYLAVERTVLTIVGLVQQ
                     KVSPKDLGGPVMIYEVTTKAAQAGWDWLLKITAFISVNLFVLNLLPLPVLDGGQVVMN
                     AVEAARGKPVSEKLLVRMQQIGIFLLIALMLFVTFNDIQRLLFGWF"
     gene            complement(11874..13040)
                     /locus_tag="PKN92_04425"
     CDS             complement(11874..13040)
                     /locus_tag="PKN92_04425"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNZ48071.1"
                     /translation="MKKITVLGSTGSIGQNTLEVVRRHPDQFQIHGLTARGNVELLLA
                     QIKEFRPHYVSVFDPELREAVQKHAGHCQVLSGPEGLVELASQPVDLVLNAVVGAVGL
                     QPLLAAIKAGNHVAAANKEPLVMAGRYIMEEAGKQGVRILPVDSEHSANFQCLEGSNH
                     ADVRCVHLTASGGPFYRYSPEEMRHISPAQAMSHPTWDMGAKISVDSATLMNKGLEII
                     EAMWLFGLELEQIRVVIHPQSIVHSLVEFVDGSFLAQLGRADMKLPIAYALNYPSRLT
                     LSDERMDLATIGTLTFDKPDCKTFPCLALAQEAAQEGGTAPAILNAANEEAVAAFCNA
                     KIPFLAIAEVVERTLSESNFSSAYDLDAVMEADRDARLRAQAHIASTSESTPLC"
     gene            13396..15594
                     /locus_tag="PKN92_04430"
     CDS             13396..15594
                     /locus_tag="PKN92_04430"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNZ48072.1"
                     /translation="MISSVSARFLSVMLLLSLLMAPLSASAEEPCKLTPEQVYPVLTQ
                     LVFVVFDLDRNDKITREEIAVISPELAQGPVGDYVAGYKNGIGRHYVNVYIKVLNPLK
                     MVDTNKDGLIQYEEVGEDVPREIFDILDHNHNDVIDCEDFDFLTEPYSQGDGEECGTE
                     EIMAIVARGGVLYLDQNNDEMLTREEIMTGISEAGDLGSDEEVFTAVFNMLDVDKDEM
                     VSAVELHATLLSIPINLMNLIDQNQDNAIQFTEVPFVPPLLFLQLDTVVNGQLDCADY
                     KAQVATEEIELPKGVIGGVVRDAATNLPLSEAVVTLTPGGFSDTSLALVGTFGFSDIP
                     YGRYTLQVVLEGYEPLTQIIRINLPITTLDLLLQPEAVEEGEVEGESPALVTLAGVVR
                     DQDTGFPISGALVTLTPGDFQEVSLDILGTFMFNEIPAGNYRLAVTKEGYQTYSTDLF
                     LESGFGDVIDVPLTAENTIEGEEQPVEGEEQPVEGEVAPAEGETGDVEGEESPEEGEV
                     QPAEGETVPDNGEKGVITGIIRNNATWLPVGKAVVRLTPGGYETTSDDISGTYLFSSI
                     PLGKYTIHVAHPAFQSYAEAVELKRGLFGVANLLALDIVLTMTEAEGEVQPAEGEVQP
                     AEGEVQPAEGEVQPVEGEVQPVEGEVQPVEGEVQPAEGEVQPVEGEVQPVEGEDVAQE
                     GEAEDEEDDGAGISGCFSSSKASPKAFLADWLLLALSFMSVGVLRSLKEK"
     gene            15648..16520
                     /locus_tag="PKN92_04435"
     CDS             15648..16520
                     /locus_tag="PKN92_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNZ48073.1"
                     /translation="MKAELECLECSLRQALRAARMACDDPAVHRQVMDAVAGDIPGMD
                     LNESPAVLSLGLYRYARELSGNPDPYKAIKAEQNAMALELEPELDALVASSGTPLLTA
                     IRLAAAGNIIDLGIMHVHEIDPRQEIMKAMNTVPAIDHSDRFMRSLESCEHLLYLLDN
                     AGEIVFDKPLIRALQKRTKVTAVVKAAPIINDACLEDAEQVGLTSLCEVIDNGGAFVG
                     SPLNLIPSEFRKRMEEADIILGKGQGNYETVDDFEGDVYLLLKIKCDVIARHCGVPRG
                     ESVFLSTRARNQKR"
     gene            16635..17645
                     /locus_tag="PKN92_04440"
     CDS             16635..17645
                     /locus_tag="PKN92_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HNZ48074.1"
                     /translation="MKRLTFDTFIPHKSNQGAYEIAREVARMQPELPRPVVFLGESGS
                     GKTHLLWAIVNYFRDRNSVVRLALISPRDFPDKVKRLSSDEDKLKAQPPIVLLVDDLH
                     GFAQHAADLEKTVLALIQHGHNAVLATRIHPNVLSGFSGKFKALLNAGAIAGIKALPK
                     AEGTIIPEAALDQILELKSKVTRLQKDKALLEAEKGGAAAAPKEDAVAPAVPQADTAS
                     SVSREHFNRALKEIQKARAETESWKKTAAAMMNRVESYKALQQSQFSSIEEKIQELLA
                     FADQDILSLDEESLHAANAEITAILARSSAEHAAALQGESQKTLRSLAEQFKALDDTL
                     PG"
     gene            17704..18294
                     /locus_tag="PKN92_04445"
     CDS             17704..18294
                     /locus_tag="PKN92_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ48075.1"
                     /translation="MHSTDQFAEAVVALFWKIHSLDRVPRAGFLLRGVPDPESVAAHS
                     HFMAFLALLFIREYPEGYDLGKALAMALVHDLPEAQLMDVPMPVASRWLGEAKEQAEQ
                     GIFDELFSAFPDYYSGLHAEFAEGKSPEARLLRALDKAQMMLKVMHYEKENRGCVEEF
                     WHNPGNFNDHGLPAVSALFDAICKKAGRTRPVPTPS"
     gene            complement(18313..19056)
                     /locus_tag="PKN92_04450"
     CDS             complement(18313..19056)
                     /locus_tag="PKN92_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNZ48076.1"
                     /translation="MAAGIRVTVWNEGIHEKEMPRCQEIYPLGMGGTIGAYLRQQPGI
                     ASVHCSQLDDPEQGLSDEILDNTDVMTWWGHKAHDQVTDENARRVQERVLKGMGLIVL
                     HSGHYSKPFTSLMGTRCNLKWREHGEHGEKERIWVVDPAHPIVEGLPEYIELPNTEMY
                     GEPFGIPQPDQLVFISWFQGGEVFRSGCCWHKEAGRVFYFKPGHESFPIYHNEYVMKV
                     IYNAVKWAAPVRMANGYTQGNYQPLEKLD"
     gene            19307..20254
                     /locus_tag="PKN92_04455"
     CDS             19307..20254
                     /locus_tag="PKN92_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNZ48077.1"
                     /translation="MAIDALQSFQGLFLLFAFLFGITVGSFCNVCIGRWPSGESVIKP
                     RSRCPKCMNAIAWYDNIPLVSWLILGAKCRHCAQPISVVYPVVEALTGLLFTLTFWRF
                     GFSAATPVYMLFCASMVIVTFQDLADWTIPNEITFPGIPFGVLLALAGTAWGEASGLR
                     VTDVTDSLLGVLLGGGILFLLDRITVYLLKKPGMGFGDVKLLAMVGAFLGWQGALGTL
                     VIASFIGSFAGIAVILYFKLRGETTDGNDGKEKQDQEGGDPEENITLQGHYLPFGPYL
                     AVGALIFMFVGPELIDYYFSFLEPSGGIVLMNDLTDFGF"
     gene            20254..21237
                     /locus_tag="PKN92_04460"
     CDS             20254..21237
                     /locus_tag="PKN92_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ48078.1"
                     /translation="MTDALFPDVRGLHTIPTPTPFPVGPANLYLYESDALYLIDTGTN
                     TPEAWEAFLVGMKKLGRDPRDIEKIILTHHHLDHIGLSRKIKDLSGAVIYANPVVLQQ
                     IPLFFQEEFTRANLEEVLFELGVPAGLNARLCDERHNFRRYLDDFTVDKLLEDGGFVG
                     PFRAFFRPGHSVSDTVLVHDKEKWAFTGDHLIHKITSNPLLRRKNEAGEREKSLIQYR
                     ESLLKTKELPITWCFAGHNTPFTDHAKSIERTLEHLESRSRKILALFNGGSATPFELT
                     QRLFPHLSDAYLYYCLSATTGHLELLEAQGRTVHDRRGGILRYSAGDLPEK"
     gene            21499..22566
                     /gene="hrcA"
                     /locus_tag="PKN92_04465"
     CDS             21499..22566
                     /gene="hrcA"
                     /locus_tag="PKN92_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNZ48079.1"
                     /translation="MSGKNQVTRSVPLNEREQVILQAIINSYVATAEPVGSRTVVKRF
                     GLDLSAATVRNVMADLEEQGYLQQVHTSSGRIPTDAGYRYYVDYLMKVQQLTQSERRR
                     IEGEYEKQLNDVDDVLRRTSHLLALVSHHAGLVEALDVEQARAQRFELMRVSDERVAV
                     LLVDNFGSVHSLATAVKPTASSEQLTRLNNFLNQNFQGATLGTLSSTIREKLGDELSE
                     QRDLAHSVLSILDMLPQRREKKLFLEGAVHLFEHPEFQSIEQAREVFGLLDEHDRLIS
                     LLRKAVSEDGGPAVFISRENGEAVGVDGIGVVASPYRVDGKPVGLIGILGPRRMPYSK
                     LTAIVQHTSEVVGRFLTRLMR"
     gene            22613..23329
                     /locus_tag="PKN92_04470"
     CDS             22613..23329
                     /locus_tag="PKN92_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNZ48080.1"
                     /translation="MDKTEFEKILERKIAEEEARKKGDETPDGIPEEVPQNAEEADAP
                     DAVSSPAQPAADSEVDLCQNALDQMTRERDALLDQLLRARADFDNYRKRSLRDMDQLR
                     ETASANLIRALLPVLDNFERALAHADSGNPLAEGVVMIQKQLLDVLSAEGLKPVEAKG
                     AHFDPHYHEALAMTPAHCAPGIVLEEYERGYLLKEQLLRPAKVIVSCEIAAESSQKDE
                     AASEQINDETAFSESEGNHD"
     gene            23367..25268
                     /gene="dnaK"
                     /locus_tag="PKN92_04475"
                     /pseudo
     CDS             23367..25268
                     /gene="dnaK"
                     /locus_tag="PKN92_04475"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            25379..26494
                     /gene="dnaJ"
                     /locus_tag="PKN92_04480"
     CDS             25379..26494
                     /gene="dnaJ"
                     /locus_tag="PKN92_04480"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNZ48081.1"
                     /translation="MPAAKDLYEILGVPKTASQEEIRKAYLKLAHKYHPDKTGGDKAA
                     EDKLKEINAAYDILKNPEKRQQYDQFGSTDGQPFGGFEGGFGGGFGGGFGDIFDMLFG
                     QQGGRQRSNRPQPGHDLEYELQISMEEAVKPLSKKISFSRKETCSDCNGSGAAKGSSP
                     ETCSQCQGSGQIRSSQGFFSMTRPCPRCSGTGQYIPRPCQNCKGQGQVRTKREISVNI
                     PAGVNTDNRLRLAGEGEAGLRGGPRGDLYIRIRIAPHPFFERRNEDVYCQAVVSFTQA
                     AAGASIRVPTLTGMGELKIPAGTQSGAQLRMRGLGFPDTRGYRQGDQFVIVQVETPQK
                     LSRKQRELLEELESLGTEKNYPLVAEYEKEIQALRKK"
     gene            complement(26506..>27165)
                     /locus_tag="PKN92_04485"
     CDS             complement(26506..>27165)
                     /locus_tag="PKN92_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HNZ48082.1"
                     /translation="SRTSYGCYGGGVGMGLGNCYEQYPGGTEGFYRFLSEGNRGVAGA
                     EHIAEALEKAGNKTMAHEFLYGERYLKNADAARRFVDTMAPREIPAAYVVMRPMEEGD
                     PDNPQVKSITFFVNADQLAALTVLSHYDTPDRERVIAPFAAGCQATALALYKEHGKEF
                     PRAIIGLMDLAARKNTRHLLPPDMLSFSLIPAMFKELESHVAGSFLETENWRNLIGSA
                     D"
BASE COUNT         6419 a         6789 c         7354 g         6603 t
ORIGIN      
        1 gaaaaaaaca gcggcacagt cctgctcagg attttcctta tgactgtgct atacttcctg
       61 tttctttacg cattacccct tcttctaccc ccttccggct gtataatgac ttttcaacag
      121 aaacaattga ttgatgaaac acgctggaca ggtacccgtg aggtgctgtc catgtccggc
      181 cctctgatcc tgggcatgct ttcctatacg atcatggagt tcttcgataa ggtcatgtgc
      241 tcctatctgg gcacagatgc actggctgcg gtagggagtg ccggaatagc ctcctttaca
      301 ctttgtacgc tttttatggg aattgccagc atcgttacca cttttgtggc gcaatgcttc
      361 ggaagagagc agtacactct gtgtgcctgt tattgctggc aggggatgta tatttccgtt
      421 ctcgggattt tttttgccgc ggtgctttgg ccgctggctc ctcttctttt cggttccatg
      481 ggacacaatg aaacagtgac ccgctatgaa ctggagtatt ttcgagcacg tgtgctgggc
      541 tatttttttg taggagcagt gacggctctt gccggttttt tccagtcggt cagccggccg
      601 cgtattccta tgtatgccgc cattatcgcc aatgcttcca atctgatact gaattatctt
      661 cttattttcg gcaagttcgg ttttccccgg atggaggtgg cgggtgccgc tgtcgccact
      721 gtgctggcaa caatcctgca gttcgccatc atgctctcct ttttcctgca caggcatttt
      781 gacagtcgtt accggacgcg tgcgtcctgg gcttttgact ggcaccgcct gcgtgagatg
      841 attaccatag ggacaccgtc ggcaataagt atgtttctgg atgtggcgaa ctggtggatt
      901 ttcacagcct ttatcatagg gcgcttcggt gcaattcaac tggctgccaa taccatcgca
      961 gtcagcttta tccacatatg ctttcttccc gctgtcggtc tgaatcatgg gattgccgcc
     1021 attgtcggtc aatggctcgg gaggcgtgat gtggagcggg caaagcggcg tacctataca
     1081 gccatcaaga tcgccggtgt ttatatggtc gccatgggca ttacttttgc ccttttcggc
     1141 gatacactga tttcgcttat cttccgcgcg gaaccggaag ttgtccatct cggacataaa
     1201 ctgctgatcc tggcagccct ttttcaggcc tttgatgctg ttaatatcgt ttgcaccggt
     1261 gcgctgaaag gggcgggcga tacacattgg atgatgtatg tgacttttgt catgtcgtat
     1321 ctgatttttc tgccgctggc actgctcttt gccttttatt tccggatggg tgcctttggt
     1381 gcctggatag gtgcaacagt gtttattatt cttttgagcg gtgtgctgtt tcgccgtttc
     1441 cggaaagaag gctggcgcaa catcaatatt tttacccgta ccgcccggga aggcacatcg
     1501 tcggctcaag ggcactgatc gccccgaagc ccggaacggc actggaggat agtactatgt
     1561 catctcttgt ccacctcact gtggatgatg gagaaaaccc tgtccgtctg gatgtgtacc
     1621 ttgcggaggc gctgaagagt ttttccagaa gccatataaa gaaactgatc cagcagggca
     1681 gcgtaaccgt aaatcaatgc atttgcacga aagctgccac agtggtgcag ccgggacaga
     1741 gtattgctgt tgaactgcct gaagcgccgc ctctggtgcc tgtaccggaa aatatcccga
     1801 taagaattct ttttgaggat cagcatctgg tggttgtgga caagccttcc gggcttgtgg
     1861 tgcatccggc gccggggcat tataccggaa cgctggtgaa tgccctgctt tttcattgcc
     1921 ctgattatga gcagaccggc gatgactccc ggcgcccggg cattgtacac cgcctggacc
     1981 gctatacctc cggtgtgctg gtggcagcga aaaatcagca tgcgtacctg catctggcaa
     2041 gacaggcatc ggaacgccat ttcgaacgtc gctatcaggc actggttgcc ggagaatttc
     2101 cggaagaccg ggggacggtg aacgcaagtc tgggcagaag cctggtcgac ccctcccgta
     2161 tggctgtcac aggcgttaat gcgaaaacag ccattaccca cttcgagacc ctggagcgtt
     2221 tcggagtcgc ctgtctggtg cgcctgacgc tggaaacggg gcgaacccac cagatacgcg
     2281 tccatatgcg ctttgcagga catcctattt ttggagaccc tgtttacggg acctgtaatt
     2341 atgacacgcc gggcgtgccg gaggagctgc tttcagcgct gatgcggctc gaaggacagg
     2401 cgctgcatgc ggaaaaactg gggtttaccc atcctgtttc cggtgaatgg ctcacttttg
     2461 tcagttcccc tcccgaagat tttcagaatg ccctgaaagc cctgcgcctt ttcaaaaaag
     2521 aatagaacaa ggatttttcc tgaaaccagt tttgtatgtc tcaggcttgt ccggggcgtt
     2581 gtattattcg taattttaac gggtgtaatg cctccgtttc gggtgaattt acctcttgct
     2641 gaaaaaggct gctttttttt ctgtttttaa accattgctt gattactacg gttaattgtt
     2701 gctaagatat ggtgcaggct gaaaaatagg tattggtcta acttccactc ctcattgcgg
     2761 gattttcccg cccatccaac tcgggacgtt ttctttgagc gcaacatcaa ccagatggtc
     2821 caaagaattt gaagatgctg tcatggagca cgttgatctg ctctatgcgg tagcgttgcg
     2881 cctgacgcgg aaccatgcgg atgcggaaga tctgacacag aatacctttg tgaaagcgct
     2941 tcgttttcat gataaattta agcccggaac ctatataaag gcctggttgc ttactatatt
     3001 gagaaatact ttcatcaacg agtaccggcg tcgctcccgc cgtccgctgg aagttgaact
     3061 gtccggactc gaagtggcac ggggcactca tcctgatccc gaggtttttt ttgagcctcg
     3121 gcccggctgc cgggaagatt tactggagct ggtggatgat cctgttcgca aagcagtgga
     3181 agccttgcct gaggaatttc gcgaagcggt tctgatggcg gatcttgaag acatgtcata
     3241 taaagagatt gctgaagcca tgggctgccc tcttggcact gttatgtccc gtctttaccg
     3301 tgggcgtaaa ctgcttcgcg acgctcttga agagtatgcc cgtgaccggg gggtcgtctc
     3361 tccgaagtaa gcagttttca gaaagatctt tcaggatttg aaagatccgg cggcacgggg
     3421 tgattttgat gcagtgatgc gccttatgtg ctttcaaatg aatagtggaa cgttttagtg
     3481 aatatcagca gaccggttct ttgccggcaa taaaatggca aattgcctgc cggtctgttt
     3541 tcaacaacaa ccaggagact cttacatggc aaagtactca gtagctcaga ttcccggcga
     3601 cggaaccggg ccggaagtga tggcggaagg cgtgaaggtt cttgatgcgg ctgccgcgaa
     3661 gttcggattt tccttttctt atcagaacta tgattttggc ggtgaacgct atatgaaaac
     3721 cggagaggta cttccggatt ccgccattga cgaattcaga aaattcgatg ccctttatct
     3781 gggtgctatc ggtcatcccg atgtcgcgcc cggcattctg gaaaagggga ttctgctgcg
     3841 cacgcgcttt gaactggatc agtatatcaa tctgcgtcct gttaaacttt atcccggtgt
     3901 tgacacgccg atcaaagata aaggacccga gcacatcgat tttgtggtga ttcgtgagaa
     3961 ctcggctgat atctacaccg gcatcggcgg tgtgcagcgc aaaggaacgc cttacgaagt
     4021 tgcgatgcag accatggttt acagccgtaa cgaagtggat cgctgcctgc gctatgcctt
     4081 cgaatgtgcc cgcaaacgca ataaaaataa caccctgacg ctttgcggca agaccaatgt
     4141 tctgacctat gtgttcggtc tctgggaacg cgctttccat gaaatgggcg aaaaagaatt
     4201 cccggatatc aagcgtgaat atgcgcacgt ggatgcaacg accatgtgga tggtgaagaa
     4261 tccggaatgg tttgatgtca ttgtgacggg caacatgttc ggcgacatta ttaccgatct
     4321 gggcgccatg attcagggcg ggatgggcat cgcggcgggc ggtaatctga accccgaagg
     4381 cgtttccatg ttcgagccta tcggcggcag cgcacccaag tacacgggta tgaatattat
     4441 caatcccctt gcctgcattt tcgccggaca gatgatgatg gatcatctgg gtgaaacaga
     4501 agccgccaag gctattgaag atgcatgtgt tgccttcctc acctccgggc tgattaaagg
     4561 acttgccgcc agcgatatta aagctgccgg gatgagcacc tcagccatcg gtgactggat
     4621 tgccgactat atcgccaaga actgattctg ctgatacttt ttcggacccg tggagaaagt
     4681 ctttcccacg ggtccctttt ttacctgaag gttttttgaa gtaacagccg cttcgccgga
     4741 tgcgaatgca tgcacaggga aggctgtcgg atacacagga tgcttcttcg agaaattata
     4801 gagcagcgcg aaatagatac actttcacca tcggcatgtt ttgcccggta ttcccggggg
     4861 cgcgccatgc ctgaggagga ggatccctac cggacagcct tccagcggga tcgtgaccgc
     4921 attctccata ccaaggcctt tcgccgcctg aaacgcaaga cgcaggtatt tcttgcccct
     4981 gaaggagacc atttccggac gcgcctcact cacacgctgg aagtctctca aattgccaga
     5041 accatttccc gggcgctctt tctgaacgaa gatctgacgg aagctatcgc attgggtcac
     5101 gatctggggc acacgccttt cgggcatgcc ggtgaaacgg tgctgaatga ggtgtacagc
     5161 gcaggattta agcattatga gcaaagtctg cgcattgttg agcatctcga aaaacgcaaa
     5221 acaggagcgg gtctgaatct cacctgggaa gtgcgtcagg ggatactcaa ccattctcac
     5281 gggaaagcaa ttttatatga ccagccggtg cctgatctgt catcggaatg tctggttgta
     5341 agccttgccg atgctatcgc ttatattaac cacgatatcg acgatgcgct gacggcaggt
     5401 gttatttcct ttgatgcgct tccaaaagaa gcagtcacgg cagtaggcga ggggaattca
     5461 aagcggatca acagtatggt gctgggtgtg attgaaggca gcagcggcgg ggaaatcaat
     5521 atcctgcccg aaattaaaag aggcatgcgt attctgcgcg actttcttta tcaggaagtg
     5581 tatccccgtg aagccataga atccgagatt cgtcaggcgc gccagatggt gcgggatctg
     5641 tattgttatc ttctggagca cccgccggca cagatgaatc atgatgatac agacgattcc
     5701 tttgaacgcc ggctcgttga ttatattgcc ggtatgacgg atgaatacgc catgcggctg
     5761 catcattcga tttttaaggg atcacagacc gggcagcgtt gataaagaga ccggaccctc
     5821 tgagtaaagc gcttcaccat ggcagacacc gatgcgcctg ccccagaaac gtgcccggtg
     5881 ctctatggat tcccagaatt ccagagagga aatataggtg ccggcactgc ggtcgtccgg
     5941 attgtaaaac tgggttttat gggcgccgat ggatgcgagt tttttcggga aaaaaccgct
     6001 gatatccaca atgccgaaag gtgaagccgc cgcatccaga tagggatgga aataatagat
     6061 gcgctccggg cggaaaggct cctgccctgt atcgatgcgc ataaggccgg acaggaaaca
     6121 ggcatctctg cccagatagt gggcggcttc atgatcaggg tgtcggtcgg gtgtcatgag
     6181 ggttaaaacc actttaggcc ggaagcgccg tataaaagga ataaccgcca gacgctgttc
     6241 cggtgtgttg gaaagaccgc cgtccggcaa tcccgcattt tcacgggcgc agagtccgag
     6301 gattgcggcg gcatgctcgg attccgcatg acgttcgtta ggggtacccc gggtgctgag
     6361 ttccccccgg gtcagatcaa gaatggcaat ccgctttcct tcagatgaaa gtttcgcaag
     6421 cagtccgccg cagccgagtt ccacatcatc gggatgggct ccgattgcca gtacatcaac
     6481 ggattcctgg tgcgtggtca tagttcaaac tcctgcacac ccgttttgtc gggagaaaat
     6541 acatccagca ttctatcgat cagcgaccac ccctgttcaa ataaaaaaga aaagagggaa
     6601 agaacccgtt cctgctcttt tccgttggga gaaaaaaaac agtgcaggcg ggtcaactgg
     6661 ttttccacag ttttccgctt ctcctgatcg gaaaagagaa gggctttccc gaaacggtca
     6721 aaataaaact gctgattttt ctggaaacgc tgcgccagct gctgaacagc tgcaggtgtg
     6781 ccgggtgcca aaaaaggcgc gaggaaatgg gcaaaagcat cgtccagcga tattcggcct
     6841 ttttgaaaag cattgtggta ccggttggcg tgctcctgtt ccaatgcacg ggtcagaacc
     6901 tgattcagat caaaaggctg cgttgtgtct gctgaaaaac gctttctcag tttgttgttt
     6961 ttgagcgtag taataagccc tttcatgcgc ggatatacga tgggcatcgg ctgatcaaac
     7021 agattaaaaa gcggttttaa ctgtgcccag taagcgattt cgccgggacc cgccacataa
     7081 gccagcacag ggaaaagcgc ctgctgaatg atcggacgca acgcaacatt gccggaaaaa
     7141 agttcgggcc gggcagccag cagggctttc atttctggtt ttgaaaaaga ccgttctgct
     7201 tcgggggcat gaaacctgcc gttttcataa aacatcgtgc agcgtatttt ttcaatttcc
     7261 aggaaaaaat tgcacatggt ttctgtgcgc cggatcggtc gcccgaaacc gcgggattcc
     7321 agaatatccc cttgccgcat gagcagtgcc gtgctttcca gcggggcttc tatttcctgt
     7381 tccagaatag cggcggcagt acggcgcgcg gcaggaagac gcggatcaaa gagacgcagt
     7441 gccgtgtggg caaaaaggcg tgcctgaata cgggaaaacc attccgctac agacgctgat
     7501 tcgccgagcg cccggataag aaaatcacgg atttcttctg tgcactcaga ggagggtgtc
     7561 accgccgctg cctgatctat aagcttgttt aactgaccgg tgaggggaat ttcgccggca
     7621 ggcatccctt ttacatatcc gtctgccgga gaaggcgtat agcggagcgt cagaagatca
     7681 tggcgccggg tcaccagtgt tgcatgattg atttcttcaa aatcatggtc gtccgaagct
     7741 gtccagaaaa gagggatgac gggggcatcc gggcggctca ccctttctgc cagctggatt
     7801 gctgtgatag ttttgtaaac tgtataaagg gaccctccga agagccctgc ctgctgcccg
     7861 gttattatga cccggcattg cggaggcaaa tcagtttttt cgacgcccag ctgctgctga
     7921 taccgattta cctgctctgc catggcggga ggaaacgggg caggggaatg cctgtcgcga
     7981 aagagagaag cgggcaatgc ttcgtaaaga tccgctactc tatcctctcc ggaaatatag
     8041 gcatgataca gactgttcat gctgcgccct tgatacccag cactagcatt ctgggtgttt
     8101 tcgtgctgta gggcgtgccg tcataattgc cccaggtttg tgtaatacgc aagcctgcct
     8161 gctccagtag aaaccgcagt tcgtcaaagg tgtacatgcg gacagattcg ctgctttttc
     8221 ctacaaggac gttgtttttt tcgacgcgga tggttttatt gacccgttgt gttttttcat
     8281 cgatccaccg ttgctcccgg atccggaaac ccagagagtc acgggtggat tcagtgacca
     8341 gattctgttt cagatattcc ggattgagat aatcaaagaa aaaacgccct cccccggcaa
     8401 gagcgtcgct catctgcaaa agtgcctttt tattttcccc gtcatccata aaatagccga
     8461 agcttgtgaa aaaagaaaag atgacagaaa aagtacgggt aaaaggaaga tatcgcatat
     8521 ccccgcgcac cagatggact tgccggcggg tgttctgcag cgcttttgcg agcagtaccg
     8581 gcgagtagtc gtaacccgtc aggctggcac cggtatccat caaagtcgca aggtgtcgcc
     8641 cggtaccgca gcacagatca agcgctgtgt ccttccggga cagacccgct gcttcagcgg
     8701 cgaaacgggc ttccggtgcg gccgaggcaa cggagcgatg agcatagaca atgttgtaaa
     8761 gatcgccgaa ggcgctttcg aaccaatctt caggaattgt ttttttctca gtcatttcta
     8821 aggtctgcag gtctgcttcc ggaaggaggc ggagaacatt actgccagct gtcaaaagcg
     8881 tgatagatgg cggaaaccgc tttttcgaga tcctgttctt ccactccgat cagaatattc
     8941 atttcagagg aaccctgatt gattacccgg atattgacgc ctgcttccgc gagtgcaccg
     9001 gtgagacgtg cggcaacgcc aaccagccgg ttcatgcctt tgcccacggt ggcgacgatt
     9061 gccagaccgg aggtgacagt gagatcgtca ggatccagaa gccgttcaat ggattccagt
     9121 atggtctgca ctttgccttt gagctcgtca tcggaggcga taatcgaaat cgtatcgata
     9181 ccggtgggca tatgttccca gctgacccgg tgctcctcga agatggaaag cagttttctg
     9241 ccgaaaccat gttctttatt catgagcgtt ttctcgatgt tgaacatggt gaagccgcgc
     9301 cgccccgcaa tgccgcatac cggatgtccg gagtcgcgcc gggcgacgat caatgtgccg
     9361 gggctgtccg gttttttagt gttcagaatc tggatgggga tgcccggttc cctgaccgga
     9421 aaaatagcct cgtcgtgaag caccgtggcg cccatgtacg agagttcgcg caattctctg
     9481 tatgtgatct cttcaatccg tttcgcatcc ggaacaatgc gcggatctgc cattctgaag
     9541 ccggacacgt cagtccagtt ttcataaagc gaggaccggg ttgcccgggc gacaatagaa
     9601 cctgaaatat cacttccgcc ccgtgaaaag gtcttgattt cccctttagg cgttgcgccg
     9661 taaaaaccgg gaaccacgaa gagaccgtcg ccttgcagtt tttcacccag caggtcatag
     9721 ctccggggat ccagctgtcc gttttctttg aaaaggatgc attctgccgg gtcaacaaaa
     9781 acggcgccca gcgcttcggc aatcaatttt ccgttgagat attcaccccg tgatgccata
     9841 tagtccggtg cttcatcacg ggaagcatgc tcggcaattt cttccagaca gggctgcaca
     9901 ttaaaagaaa cgcccagctc gcgggcaagg gtcgaaaaac gttcttctat aatgcttttc
     9961 ggttctgtcg gatcaagacc ctgccgtgcc atattgtgcc acgcatacag aaggtctgta
    10021 atctttttct cttctgctgt tcgcttgccc ggggcggaag gcacaatata cttccgtgcc
    10081 gggttggcac ggataatgtc gataacctga cggatgcagg atgaatctgc cagagaagag
    10141 ccaccgaatt tgcaggtgat acgctccatg gttgtctttc ctcttacact gtaattgggt
    10201 taaattctga ggggattaaa accagccgaa aagaaggcgc tgaatatcgt tgaatgtgac
    10261 aaagagcata agtgcgatca ggagaaaaat gccgatctgc tgcatgcgaa ccagcaattt
    10321 ttcactgacc ggtttgcctc ttgccgcttc caccgcattc atgactactt gcccgccgtc
    10381 gagaacaggc agcgggagaa gattcaggac aaagagatta acactgatga aggctgtgat
    10441 tttgagcagc cagtcccatc ccgcctgggc ggcttttgta gttacttcat agatcatcac
    10501 cggcccgccg agatctttgg ggctcacctt ttgctgcaca agcccgacaa tcgtcagcac
    10561 cgtgcgctcc acggcgagat aactctgata gaaggagtgg gggatgatgc gcactactgg
    10621 cacccgatgc cagactgttt tcgggcgcaa cgctacacct atagcgccga cagcctggat
    10681 tttcagggag gcgttgatct tttgctcagg ctccaccaca ccgaaaagaa tgcccggtct
    10741 ctgaacggac agatcaaatt ccgagcccgg attctctttc tcaaaggtaa aaaattctgc
    10801 aggactcatg gcttttccgt tgactgccgt aaccatatct cccgctttca gtccagattc
    10861 ttcagcggtt tttgtttcta tggcaagtat gcgcagttcc tgctcttttt cggaggaacc
    10921 gaaagagatg ccttttatgc gtccgattgt ttcaggagta gcttgcaacg ccagggtctc
    10981 gccgtttcgc tccacctcca gccggacgct ttcaccggct ggtatcttct ctatagatgt
    11041 aataaatgaa gtcagggaaa cggtcttatc gttaaccgcc cggatgatgt cctgaggctt
    11101 caggcctgct gcttctgcct ccgagccggg gaatacctcg cggacaatgg ctgtctcaaa
    11161 aggaccgacg ccgaagcgtg gatgccccga agcatcgagc acctgaggga cgacaggaga
    11221 cacaaaacgt ctgccgtcaa ttctttcaag cagcacgtcg tatgttttct tgtcaccgcc
    11281 cagcactgcg gtaatggcga gatctgtcat gttgccgatt tcccggccgt ttacacttag
    11341 tatgcggtcg cccacctgcc agccctgagc atcgggagtg cctgtcatgt cggcagcgtc
    11401 ggtgttgtga agcaggaaaa gaggagccgt ttctaccggc gaacctgctt caatttcgcc
    11461 tactcgtgcc tccacttccc attcgggtac ttcactgcca acgagtccta ccagtgcata
    11521 caaaacaacg gcaagaacaa agttcatgga aggtcccgca agcagcacgc acatccgctg
    11581 aatgacgggt ttgtttttaa accagcggtc ttccggcaca tgtccgtatt cctcttcgcg
    11641 ttcttcatcg ctgaggggaa catcttcctg acccgccatc atgacaaagc cgccgatcgg
    11701 gagcgctcct atacagtaat ctgtttcgcc ccactgaaag ccgaaaatac ggggcggcat
    11761 accgatactg aaccgtttta cataaatgcc gaaacttttt gccgccagaa aatgccccag
    11821 ttcgtgaatg aaaatcagaa ggcttaacac ggcaacaaat atgacaatat taatcagcat
    11881 aaaggagtac tttcggaggt tgaagctata tgagcctgtg cacgcagccg tgcgtcccgg
    11941 tctgcttcca tcacggcatc cagatcataa gcggaggaaa agttactttc agacagcgtc
    12001 cgttcaacga cttccgcgat agccagaaag ggaatctttg cgttacaaaa agcggctacc
    12061 gcttcttcat tggcggcatt cagaatagcg ggtgctgtgc ctccctcctg tgctgcttcc
    12121 tgagccagcg ccaaacaggg aaatgttttg cagtccggtt tgtcaaaggt cagggtgcca
    12181 attgtcgcca aatccattct ttcatcggat aacgtcagac gggagggata gttgagagca
    12241 taggcaatag gcagcttcat gtctgcccgc cccaactgcg ctaaaaaaga cccgtccaca
    12301 aactcaacga ggctgtgaac aatactctgg ggatgtatta ccacccttat ctgctccaat
    12361 tctagcccaa acagccacat ggcttcaatt atttccagtc ccttgttcat gagcgtggct
    12421 gaatctacgc tgattttagc gcccatatcc caggtggggt gagacatggc ctgcgccgga
    12481 gagatgtgtc gcatctcctc cggagaatat ctgtaaaagg gacctccgga cgccgtcaga
    12541 tgcacacagc gtacgtctgc atggttggag ccttccaggc attggaagtt ggcgctgtgt
    12601 tcgctgtcaa caggcaggat gcgcacgccc tgttttcctg cttcttccat gatgtaccgg
    12661 cctgccatga ccagaggttc tttattggct gccgccacgt gattcccggc tttgatcgca
    12721 gcgagcagcg gctgtaaacc gaccgcccct accactgcat tcagtacgag atctaccggc
    12781 tgcgatgcca attctacaag cccttccggg ccgctgagca cctgacagtg cccggcatgc
    12841 ttctgcaccg cttcgcgcag ttccggatca aaaacggata catagtgcgg tcgaaattct
    12901 ttgatctgtg ccagaagtaa ttccacattt ccccgggcgg tcagcccatg gatctgaaac
    12961 tgatcaggat gccggcggac aacttccagc gtattttgcc cgatactgcc tgtggaacct
    13021 aaaacggtta ttttcttcat aattaccctg aaaagaggcg gaaggtgctt tatgcgcatg
    13081 caaggcatga aacccgaaag aactgctgaa agcatactat attattgccg tgcttctatt
    13141 gtcaattgag cctgacaatc tgcccggcaa agtgttcgcc aattttgagt atgaacttat
    13201 tgcaaaattc aaaattcata gatataataa tattgagaag ggtgttcggc gcaggtgggg
    13261 acagacatat catctcaaat ttttatgctg tgtaaagaac agcagatccc tgtccggcta
    13321 cgatagaggc acttcctgac cggaaaagtt cagggaactg ttcctgaaag accctcatta
    13381 actaaggaaa gagaaatgat ctcatccgtt tccgcaagat ttctcagtgt catgctgctc
    13441 ctctcccttc tgatggcgcc tctctctgca tccgctgaag agccctgcaa gctgacgccg
    13501 gagcaggtat atcctgtctt gactcagctg gtatttgtgg tttttgacct tgaccggaac
    13561 gataaaatca cgcgcgaaga aatagccgta atttctccgg aactggcgca aggtcccgtc
    13621 ggtgactatg tggcggggta taaaaacggt atcggccgcc actatgtcaa cgtctatatt
    13681 aaggtattga accctctgaa aatggtcgac acgaacaagg acggtctgat ccaatacgaa
    13741 gaagtgggag aggatgtgcc ccgcgagatc tttgatatcc tggatcataa tcacaatgat
    13801 gtcattgact gcgaagactt cgattttctg acagagccgt acagccaggg ggatggagaa
    13861 gaatgcggca ccgaggaaat tatggcaatt gttgcccggg gtggcgtcct ctatctggat
    13921 cagaataatg atgaaatgct cacccgggaa gaaataatga ccggaatttc tgaagccggc
    13981 gatctgggat ctgacgaaga agtgttcacg gctgtattta acatgcttga cgttgataaa
    14041 gacgagatgg tttccgccgt ggaactgcat gccacattgc tgtccatacc gattaatctg
    14101 atgaatctca tagaccagaa tcaggataat gcaatccagt ttactgaagt tccttttgtg
    14161 ccgcctcttc tgtttctgca gttggacacg gtggttaacg ggcagcttga ttgcgctgat
    14221 tacaaggcgc aggtggcaac agaagaaatt gaattgccga aaggtgttat aggcggtgtg
    14281 gtgcgtgatg cagctaccaa ccttcctctc tctgaagcag ttgtgacgct gacccctggc
    14341 ggattctccg atacgagtct ggcgctggtc ggcaccttcg ggttttctga catcccttat
    14401 ggtcgctata cacttcaggt tgtcctcgag ggctacgagc cattgacaca gatcatacgc
    14461 atcaatctgc cgatcacgac tctcgacctt ttgctccagc ctgaagcagt ggaagaaggc
    14521 gaggtggaag gcgaatctcc ggctctcgtg acgcttgccg gcgttgttcg tgatcaggat
    14581 acagggtttc cgatctccgg cgcactggtt accttgactc ctggagattt ccaagaggtt
    14641 tctttggata ttctggggac ctttatgttc aatgaaattc ccgcagggaa ctacaggcta
    14701 gccgtaacaa aagagggata ccagacctac agtacagatc tttttcttga gagcggtttc
    14761 ggtgatgtga tagatgttcc cctgactgct gaaaatacaa tagagggcga agagcagcct
    14821 gtcgagggcg aagagcagcc tgtcgagggc gaagtggcgc ctgccgaagg cgaaacaggc
    14881 gacgtagaag gcgaggagtc tccggaagag ggcgaagtgc agcctgccga aggcgaaacg
    14941 gtgcccgaca acggagaaaa aggtgttatt accggaataa tcagaaacaa cgcgacctgg
    15001 ttgccggtgg gaaaagcggt ggtacgtctt acaccgggcg gatatgagac aacaagcgac
    15061 gatatatccg ggacatacct cttttcgtca atccctcttg ggaaatatac cattcatgtg
    15121 gcacatcctg catttcaaag ctatgcagag gcagtggagc ttaaaagagg cctgttcgga
    15181 gttgcgaatc ttctggctct ggatattgta ttgacgatga cagaagcgga gggtgaagtg
    15241 cagcctgcgg agggtgaagt gcagcctgcg gagggtgaag tgcagcctgc ggaaggtgaa
    15301 gtgcagcctg tcgagggtga agtgcagcct gtcgaagggg aagtgcagcc tgtcgagggt
    15361 gaagtgcagc ctgcggaggg tgaagtgcag cctgtcgagg gtgaagtgca gcctgtcgaa
    15421 ggggaagacg ttgcgcaaga aggcgaggcg gaagatgagg aagatgatgg cgccgggatc
    15481 tccggctgct tcagttcttc caaggcatcg cccaaagcat ttctcgcgga ttggcttctg
    15541 ctggcgctga gctttatgag tgtcggtgtt cttcgttctc tgaaggaaaa ataaaacagt
    15601 ccgattcaac tttttgaatt agattgatct gtgattgaaa ggtagatgtg aaagctgaat
    15661 tggagtgttt ggagtgttcg ctccggcagg cacttcgtgc cgcccgtatg gcatgtgatg
    15721 atcctgctgt gcaccgtcag gtgatggatg ccgttgccgg cgatattccc ggaatggatt
    15781 taaacgagtc gcccgctgtt ctttccctgg gtttgtaccg ctatgcacga gagttgtccg
    15841 gaaatccgga tccctacaaa gccatcaaag cggaacagaa tgccatggcg ttggaactgg
    15901 aaccggagct tgatgccctg gttgcttcca gcggaacacc gcttctgacg gcaatacgcc
    15961 ttgccgctgc cggcaatatc atagaccttg gtattatgca tgtccacgaa attgatcccc
    16021 gccaggagat catgaaggcg atgaataccg ttcccgccat agaccatagc gaccgcttta
    16081 tgcgaagtct ggagtcctgc gagcatctgc tgtacctgct tgacaatgcg ggtgaaatcg
    16141 tctttgacaa gccgcttatc cgggcactgc agaagcggac taaggtgact gctgttgtga
    16201 aagccgcacc catcattaac gatgcatgtc tggaggatgc ggaacaggtg ggactcactt
    16261 cactgtgcga agtaatcgac aacggcgggg cttttgtcgg ttctccgctg aatctcattc
    16321 cttctgaatt tcgaaagaga atggaggaag ccgatataat tctcggcaag ggtcagggca
    16381 attatgaaac agtggatgac ttcgaaggcg atgtctatct tcttctgaaa attaaatgtg
    16441 atgtgattgc ccgccattgc ggggtgccta ggggagaaag cgtttttctg tcaacgcgcg
    16501 cccgcaatca gaagcgttga cacgtctatt acatcccggt ataatcgttt catcatcctt
    16561 gctgtcggcg tcttttgaaa gattcctgtc tgcgcaaccc gacgtttcac gtcaacatga
    16621 aggaagcgat gcctatgaaa aggctgactt tcgatacctt tatacctcat aaaagcaatc
    16681 agggtgccta tgaaatagcc cgggaagtcg caagaatgca acctgagctt ccgaggcccg
    16741 ttgtgtttct gggtgaatcg ggatcaggca aaacacacct tctctgggcg attgtgaatt
    16801 attttcgtga ccggaacagc gttgttcgcc tggcgttaat ttctccccgt gatttccccg
    16861 acaaagtgaa gagactttct tcagatgaag ataaattaaa agcccaaccg cccattgtcc
    16921 ttctggtgga tgacctgcat ggttttgcac aacacgccgc agatctcgaa aaaacagtac
    16981 tcgctcttat tcagcacggc cataacgccg tactcgcgac ccggattcat ccgaatgttc
    17041 tttccggatt cagcggaaaa ttcaaggcac ttttgaatgc cggcgccatt gcaggtatta
    17101 aggcacttcc gaaagcggaa ggcacgatta tccctgaagc cgctctcgat cagattttag
    17161 agctgaaatc aaaagtgacc cgcttacaga aagacaaggc cttgctcgaa gcggaaaaag
    17221 gaggagccgc ggcggcacct aaagaagatg ccgtcgctcc ggcggtgcct caggctgata
    17281 cagcctcttc cgtttcccgg gaacacttta atcgggcact taaggaaata caaaaggcgc
    17341 gcgcagaaac tgaatcctgg aaaaagacag cggcggctat gatgaatcgg gtggaaagtt
    17401 ataaagcact gcagcaatct caattcagca gtatagaaga gaagatccag gaacttcttg
    17461 cttttgccga tcaggacatt ctgtcgcttg atgaagaaag cctgcatgct gccaatgctg
    17521 agataacggc tattctcgcg agaagcagcg cggagcatgc ggctgcgctg cagggagaaa
    17581 gtcagaaaac gcttcgttct cttgccgagc agttcaaagc gttggatgat acgcttcccg
    17641 gataacttcg ttctttttct tgccgctctc ttctttcgtt gtatctcccg aaaggtcttt
    17701 gtcttgcatt caaccgatca atttgctgaa gctgtggttg ctttgttctg gaaaatccat
    17761 tctcttgacc gggtgccccg tgccggtttt cttctccgag gggtgcccga tcctgaaagc
    17821 gtggcggctc acagccattt tatggctttt ctcgcattgc ttttcatccg ggaataccct
    17881 gaagggtatg atctcggcaa agcgcttgcc atggcacttg tgcatgatct acccgaagca
    17941 cagctcatgg atgtgcccat gcctgtggca agcaggtggc tgggggaagc gaaagaacag
    18001 gcggagcagg ggatttttga tgagctgttt tccgctttcc cggactatta ttcggggctt
    18061 catgccgagt ttgcagaagg gaaaagcccg gaagcccggc tgctccgtgc tctggataaa
    18121 gcgcagatga tgctgaaagt gatgcactat gaaaaagaga accggggctg tgtagaggag
    18181 ttctggcaca accccggcaa ttttaatgat catggtctgc cggctgtatc ggctcttttt
    18241 gatgccatat gcaaaaaggc aggccgcaca cgcccggttc ccacaccctc ctgaggggag
    18301 gagcggatca gatcagtcca gcttttccag aggctggtaa ttgccttgcg tgtacccatt
    18361 agccatgcgc acgggcgctg cccatttaac ggcgttgtag attactttca tcacatattc
    18421 gttatggtaa atgggaaaac tttcgtgccc gggtttaaag taaaagaccc gccccgcttc
    18481 tttatgccag cagcagccgc tccggaatac ttcgccgccc tgaaaccagc tgataaatac
    18541 cagctgatcg ggctggggga tgccgaaagg ttctccgtac atttccgtgt tcggcagctc
    18601 gatgtattcc ggcagccctt ctacgatggg gtgcgccgga tccactaccc agatccgttc
    18661 tttttcgcca tgctccccgt gttcacgcca tttaagattg cagcgtgttc ccatgagaga
    18721 ggtgaagggc tttgagtagt ggcccgaatg cagaacaatc agtcccatgc cttttaaaac
    18781 acgttcctga acccgacggg cattttcatc ggtaacctga tcatgcgctt tgtgacccca
    18841 ccaggtcatg acatcggtgt tatccagaat ttcgtccgaa agaccctgct ccggatcatc
    18901 cagctgcgaa caatgcactg aggcaatgcc cggttgctgt cgcaggtaag caccgatagt
    18961 gcctcccatg cccagcggat agatctcctg gcatcggggc atctcttttt catgtattcc
    19021 ttcgttccat acagttacac ggattccggc agccatagtg ctactccttt tctggcaggg
    19081 ttgaattggt tttgtttgca tgcgcattca cagggcgtgc aggctgaaaa aacggcgtgc
    19141 cgccggatat gcaaggaatg ctgtttcact cttggacggg cacaatcata gcatcagaat
    19201 agcgcaccgg cgcaactgtg cgcccatggt ggcttttcct tcaagttgcg ctgatagcgc
    19261 agtaaaccgt atactttctt caaacggggc aatcaaaagg aaaatcatgg ctatagacgc
    19321 attgcaatca tttcaagggc tgtttctgct gtttgccttc cttttcggca tcacagtcgg
    19381 cagtttctgc aacgtgtgta tcggcagatg gccttcaggc gaatcggtca taaaaccgcg
    19441 gtcgcgatgc cctaaatgca tgaatgccat tgcctggtat gacaacatcc cccttgtcag
    19501 ctggctgata ctgggagcaa aatgcaggca ttgcgcacag ccgatcagcg ttgtttatcc
    19561 cgttgtggaa gcactgaccg gtcttctctt cacgctcacc ttctggcgtt tcggtttttc
    19621 agccgccacg cccgtctaca tgctcttttg cgcctccatg gtcattgtta cctttcagga
    19681 tctggctgac tggacgatcc ccaatgagat tacctttccg ggcatcccct tcggagtgct
    19741 gctagctctt gcaggaacag cctggggaga ggcgtcgggg ctgcgtgtga ccgatgtgac
    19801 ggattccctt ctcggcgtgc ttctcggcgg cggcattctt tttctgcttg accggatcac
    19861 ggtgtacctg ctgaaaaagc cgggtatggg cttcggagat gtaaaactgc ttgccatggt
    19921 tggcgctttt ctcggatggc agggggcatt gggtacgctg gtcattgctt ccttcatcgg
    19981 cagctttgcc ggaattgccg tgattcttta ttttaaactg cgtggtgaaa caaccgatgg
    20041 caatgacggc aaagagaaac aggatcagga aggtggcgat ccggaagaga acatcacgct
    20101 tcaggggcac tatcttccct ttggtcctta tcttgccgtt ggcgccttga tttttatgtt
    20161 tgtcggaccg gagctcatcg attattactt ttcatttctc gagccttcgg gcggtattgt
    20221 acttatgaat gatttgacgg atttcggatt ctgatgactg atgccctttt tcctgatgta
    20281 cgggggttgc atacgattcc gactcctacg ccctttccgg ttgggccggc gaatctgtat
    20341 ctctatgaga gcgatgcgct ttatctgatt gatacgggaa ccaacacccc ggaagcgtgg
    20401 gaagcctttc ttgtcggtat gaaaaaactg gggcgcgacc cgcgcgatat tgaaaaaatt
    20461 attcttacgc accaccatct ggatcatata gggctgtcca gaaaaataaa agatctgtcc
    20521 ggcgctgtta tttatgccaa cccggtagtg cttcagcaga ttcccttgtt ttttcaggaa
    20581 gaatttaccc gtgccaatct cgaagaggtg ctttttgaac tgggggtacc tgccggtctg
    20641 aatgcccgat tatgtgatga acgccataat ttccggcgct atctggatga tttcacggtg
    20701 gataaactgc tggaagacgg aggatttgta ggacctttcc gtgctttctt tcggcccggc
    20761 cactcggtga gcgataccgt actggttcat gacaaggaaa aatgggcttt taccggtgat
    20821 cacctgatcc ataaaatcac atcgaatccg ctgctcaggc gaaaaaatga ggcaggggaa
    20881 cgtgaaaaaa gcctgattca gtaccgggaa tcccttctga aaacaaagga actgcccatt
    20941 acctggtgtt ttgccgggca caatacacct tttaccgatc atgcgaaaag cattgagcgg
    21001 acactggagc atctggagag cagaagccga aaaatactcg ctctgtttaa tggaggcagt
    21061 gccacaccat ttgagctgac gcagcggctc tttcctcatt tgtccgatgc ctacctttac
    21121 tactgcctgt cggcgacaac cggccatctg gaattgctgg aagcacaggg gcgcacggtg
    21181 cacgacagac gtggcggtat cctgcgctat tctgccggcg acttgccgga aaagtaagca
    21241 ctacaggaca gaacctgccc ctgttgcaaa tgtgaagcct gaatgtcccc gatttacaga
    21301 ggataaaaaa ttttaccccc gggaatccgg atttcgtgcc ctgttccgtc tccatcttta
    21361 cgcatagagt ggcactattc ttgacgcacg ctttaatata tggtacacta caactgttag
    21421 cactctcctt tattgagtgc taaatgcacc atcctgcgga aggtagtggt atcatgccat
    21481 gccacaggag tacccaaaat gtcaggaaaa aaccaggtga cccgctctgt tccgctcaat
    21541 gaacgggaac aggtgatcct tcaggcaatc attaacagtt atgttgctac ggcggagcct
    21601 gtcggctccc ggactgtagt caaacgtttc ggtctggatc tgagcgctgc tacggttaga
    21661 aatgtaatgg ctgatctgga agagcagggc tatctccagc aggttcacac cagttcaggc
    21721 aggattccta ccgatgcagg ctatcggtat tatgtcgatt acctgatgaa agtgcagcag
    21781 ctgacccagt cggaacgccg ccgtatcgaa ggagagtatg aaaagcagtt aaatgatgtg
    21841 gacgacgtgc ttcgccgtac cagccatctg cttgctctcg tttcacatca tgccgggctt
    21901 gttgaggcac tggatgtgga acaggcaaga gcgcagcgtt ttgagttgat gcgtgtcagt
    21961 gacgagcgtg ttgccgtgtt gctggttgat aatttcggct ctgttcactc tcttgccacc
    22021 gcggtaaaac cgaccgcaag cagtgaacaa ctgacgcggc tgaataattt tttaaatcaa
    22081 aattttcaag gcgcaacgct gggaacactg tcgagcacta ttcgtgaaaa gcttggcgat
    22141 gagctttccg agcagcgtga ccttgcgcac agtgtgctga gtatccttga tatgctgcct
    22201 cagcgccggg agaaaaagtt gtttctggaa ggtgctgtac atctttttga acatccggaa
    22261 tttcagagca tagagcaggc gcgggaagtc ttcgggcttc ttgatgagca tgatcggctt
    22321 atttcgcttt taagaaaggc tgtatcagaa gacggcggtc ccgctgtgtt tatcagccgt
    22381 gagaacggcg aagcggtagg ggtcgacggt atcggggtag ttgcctcgcc ttatcgggtg
    22441 gatggaaagc ctgtcgggct catcggcatt ctcgggccgc gccggatgcc ttattcaaag
    22501 ttgacggcca ttgtgcagca tacttccgaa gtagtagggc gttttctgac ccggttgatg
    22561 cgataaccgc aaatgaagtt aaaaaaaata ctgttaacag atgagtgatt cgatggataa
    22621 aactgaattt gaaaaaatac tggagcgtaa aatagcagaa gaagaggcac gaaaaaaggg
    22681 tgacgaaaca cctgacggta taccggagga agtgccacaa aatgcagaag aggcagacgc
    22741 acccgatgcg gtgagctctc ctgcacagcc tgcagcggat tccgaagtgg atctgtgcca
    22801 aaacgcactg gatcagatga cgcgggaacg tgatgcgctt cttgaccagc ttttgcgtgc
    22861 tcgcgctgat ttcgacaact acagaaagcg cagccttcgg gatatggatc agctgcgcga
    22921 aacagcatcg gcgaatttaa tcagagcatt gcttcccgta cttgataatt ttgaacgggc
    22981 gctcgcccat gcggattccg gaaatcccct ggctgaaggg gtggtcatga ttcagaagca
    23041 gctgctggat gttttgagtg ctgaaggtct gaagcccgta gaagcgaagg gggcgcattt
    23101 tgacccccac taccatgaag cacttgccat gacgcccgcc cactgcgccc cgggtattgt
    23161 gttggaagaa tatgagcgtg ggtatctgct gaaggagcag ctgctgcgcc ctgcgaaagt
    23221 gattgtaagt tgtgaaattg cagccgagtc atcgcagaaa gatgaggccg cttctgaaca
    23281 gataaatgat gaaacagcgt tttccgaatc agagggaaac catgactgat agtgtgctga
    23341 tacacacgta aaggagagat ttaaccatgg gaaaagttat cggaatcgat ctgggaacga
    23401 ccaattcctg tgttgccgtg atggaaggcg gtgagcctgt tgttatcacc aatgccgagg
    23461 gtaaccgtac cacgccttct gttgttgctt tcaataaaga cggagaacgg cttgtgggtg
    23521 ctgttgccaa acgtcaggcg gttacgaatc cgcaaaacac gattttcagt atcaagcgct
    23581 ttatggggcg gcggcacagc gaagttgcgg aagaagaaaa gatcgttcct ttcagcgtga
    23641 cttccagtgc ctcgggcgac ctgcaggtca acattcaggg aaaatcctat cgtccgcctg
    23701 aaatttctgc catggttctt cagaaaatga aagagacggc agaggcctat ctgggtgaaa
    23761 cagtatctca agcggtgatt acagtcccgg catattttaa cgactcacag cgccaggcga
    23821 cgaaagatgc cgggcgtatt gccggtcttg aagtgctgcg catcatcaac gagccgaccg
    23881 ctgccgccct cgcctatggc atggaaaaaa agaaagatga aaaagttgta gtctacgacc
    23941 tgggcggcgg aacctttgac gtttctgtcc tttccatcgg tgatgacagt ttcgaagtgc
    24001 tcagcaccaa cggtgatacc catcttggcg gagacgattt tgaccagaag gtgatcgact
    24061 ggctggcaga agagtttctc cgtgatcagg gtattgatct gcggaaggat cccatggcgc
    24121 tgcagcgcct gaaagaagcg gcggaaaagg caaaatgtga actctccagc gccgctacca
    24181 ctgacattaa cctgcccttt attacggcgg atcaggcagg acccaagcat atgaattaca
    24241 cgctcagccg ggcgaaactc gagcagcttt gcgacgcact gctgcagcgc accaaaaatc
    24301 cctgcttccg tgctctggaa gacgccggac tgcaggcacg cgacatcaac gaagtaattc
    24361 ttgtcggcgg tatgacacgc atgccggcgg tgggtcgtat cgtaaaagaa gtgttcagca
    24421 aagagccgca ccgcggtgtg aaccccgatg aagtggtcgc actgggtgct gccattcagg
    24481 cgggcgttct ttccggcgat gttaaagacg ttctgcttct ggatgttacc cccctgtcgc
    24541 tgggtattga aaccctcggc ggtgttacca caaaactgat tgaacgcaat accaccatcc
    24601 cggtaaccaa gcgtcaggtt ttttccacgg cagcggataa tcaggaagcc gttactattc
    24661 atgtgctcca gggtgagcgc gaaatggcag ccgataaccg cacgctgggc aagtttgatc
    24721 ttttaggtat tccgccggcg ccccggggca ttccccaaat cgaggtgtcc ttcgacattg
    24781 atgccaatgg gattgtgaat gtttcggcaa aggatcttgg aacaggcaaa gagcaggcga
    24841 tccgcattga gtcttccagc ggtctttctg aagaagacat cgacaaaatg atgcgtgatg
    24901 ctgaaaagca cgccgaagag gacaaagaac gccgcaaact cgttgaagtc cggaataacg
    24961 ccgacgcaat ggtgtactcc acagaaaaaa gcctgaaaga gcatggcgac aagatggaag
    25021 cgcaggacag agcgaatctt gaatccgcca taagtgcact gaaagaggcg cttaaaggcg
    25081 acgatgtcaa agcaattgaa tcggcgctcg aaaaggcaaa tacagctgct cataaacttg
    25141 cggaagtgct gtatagagaa gcgtcgcagg cgcagaatgc tgccggagga agtcatcagg
    25201 gcggtgctga atatgtacgg cccgaagatg cgggcgccaa caatacaaca gatgccgact
    25261 ttacagtaat ggatgatgac gacagcagta ataattgatt tggcctgcgc catcgttttg
    25321 ttgggattga acatacaccg cagaagcggt agagaaactc tgcaggaaag tcattgttat
    25381 gcctgctgca aaagatttat atgaaatact gggcgtgccc aaaacagcca gccaggaaga
    25441 aatccggaag gcgtacctga agcttgccca taaatatcat cccgacaaga cgggaggcga
    25501 taaagctgca gaagacaaac tgaaggagat taatgccgcc tatgacatac tgaaaaatcc
    25561 cgagaaacgg cagcagtatg atcagttcgg cagcacggac ggacagcctt tcggcggttt
    25621 tgaagggggc ttcggcggtg gtttcggcgg cggcttcggt gatatttttg atatgctttt
    25681 cgggcagcag ggcggaaggc agcgctccaa ccgccctcag cccgggcatg atctggaata
    25741 tgaactgcag atttctatgg aggaggccgt aaaacctctc agcaaaaaaa tcagcttctc
    25801 ccgcaaagaa acctgctcgg actgtaatgg cagcggtgcc gccaaaggtt cgagtccgga
    25861 gacttgctcc cagtgccagg gatctgggca gatacgcagc tcacagggct ttttcagcat
    25921 gacaagaccc tgtccgcgct gctccgggac aggtcagtat attccccggc cctgccagaa
    25981 ctgcaaaggg caggggcagg tgcgtacgaa acgggagatc agtgttaata ttcccgcagg
    26041 agtgaataca gacaatcgtc tccgtcttgc cggtgaagga gaagccgggc tgcgcggagg
    26101 gccccgggga gacctctaca ttcgtatcag gattgcgccc catcccttct ttgaacgacg
    26161 caacgaagat gtgtactgtc aggcggttgt gtcttttaca caggctgccg caggtgcctc
    26221 tattcgcgtg ccgaccctca cagggatggg ggagcttaag ataccggccg gaacgcagtc
    26281 aggcgcccag ctacgaatgc gcggcctggg tttccctgat acccgggggt accgtcaggg
    26341 ggatcaattc gtgattgttc aggtggaaac accgcagaaa ctctccagaa aacagcgtga
    26401 actgctggaa gagcttgaaa gtctgggaac agagaagaat tatccgcttg tagccgaata
    26461 tgaaaaagag atacaggcgc tgcgtaaaaa gtagcttctg tttcttcagt ctgcgcttcc
    26521 gatcaggttc cgccagttct ccgtttccag aaaactgccc gctacatgag actcaagttc
    26581 tttgaacatt gccgggatca gtgaaaaaga aagcatatcc ggcggaagca gatggcgtgt
    26641 gtttttgcgt gccgccagat ccattaagcc gatgatagcc cgggggaact ctttcccgtg
    26701 ttctttataa agagccaggg ccgttgcctg gcatccggcg gcaaaaggcg caatgactcg
    26761 ctcgcgatca ggcgtgtcgt agtgactgag aactgttagc gcggcgagct gatctgcgtt
    26821 tacaaaaaag gtaatgctct ttacctgagg gttgtccgga tctccctcct ccatagggcg
    26881 cataacgaca taggccgccg gaatttcccg gggtgccatg gtatcgacaa aacgtcgtgc
    26941 cgcatccgca tttttaagat accgctctcc gtacagaaac tcatgcgcca tagtcttgtt
    27001 gcccgctttt tcaagggctt ccgcaatgtg ctccgctccg gctactcccc ggtttccttc
    27061 tgacaggaag cgataaaagc cttctgtgcc gcccggatac tgttcgtagc aattgccaag
    27121 ccccatgccg acaccgccac cgtagcaccc gtagctggtt cgtga
//
LOCUS       DAOPWB010000030        26760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1797_length_26760_cov_5.229021, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000030 DAOPWB010000000
VERSION     DAOPWB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 26760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26760
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1797_length_26760_cov_5.229021"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            483..1661
                     /locus_tag="PKN92_04490"
     CDS             483..1661
                     /locus_tag="PKN92_04490"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HNZ48083.1"
                     /translation="MLLKRAVHFGAGNIGRGFLGQLYCESGYATVFIDVESEVVDALN
                     SRGSYTVDLVSDEGTETRTISAVSAVHASDPDKAAVTLAQASIASTAVGMHALPHIAP
                     VIAASIPLRFSDPHAQPLDIILCENIHDGEAHMRALVRKHLPADWHQVLDEKVGFVEA
                     SIGRMVPTRTRAQQEAYILAVAVEPYCELPVDAHAFRGALPPVKHLLPTANFKGYVER
                     KLFVHNLTHAATAYLGYRRGYTYISEAIRDAEVRKSVEGAGLESCRALVQKHGLDAES
                     LESHRQDLIRRYHNRALADQVERVARDPRRKLGRDDRLIGAMLLCLDQGIVPDSIASV
                     TAAALTYDNPVDPVALELQKYLRASGLDAVLKDICRIDPGSDAAGLIKKYYNKKQSTN
                     "
     gene            1676..2998
                     /locus_tag="PKN92_04495"
     CDS             1676..2998
                     /locus_tag="PKN92_04495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNZ48084.1"
                     /translation="MHEILLPKMGNSVEEAEIVKWFKKEGDAVETGDPLFSIQTDKAE
                     VECESTASGILRKILVPEGVEVPVLTVVALVGDADEALPDNMGAPSASASAPAAQVSA
                     PITGPAAAAPAAPVSPGTGATGKSPVSPRARKAAEALGINPATLSGSGVGGRVMEQDV
                     LASARSGGAVKITPTARRMAENAGLSVAQLSGTGIGGKITKADIARSLEQGIAQAAPE
                     AKEGIRTVPLSPMRRIIADRMCASKYSAPHYYITVEVDMSAAKNFRAANRGFKVSYND
                     LVLFATARALRSFPAVNARWCGDFIEELSEVNLGMAVALPTGLIVPVIRNAHLLSLEG
                     LSSTAKALAEKAQTGKLLPDDYQGNSFTVSNLGAFGVDQFTAIINQPDSAILAVGQMK
                     DRAVVIDGGIHIRPVMKLTLSSDHRVIDGAVAAQFMSHLKSILEEGAF"
     gene            3022..4410
                     /gene="lpdA"
                     /locus_tag="PKN92_04500"
     CDS             3022..4410
                     /gene="lpdA"
                     /locus_tag="PKN92_04500"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNZ48085.1"
                     /translation="MKNYDVVVIGAGPGGYVAAIRAAQRGARTAVVEYRNLGGVCLNE
                     GCIPTKTLIHTAELYRKLQNAAEFGIEVRDVRVNVRKMLDRKETVIGINTGGIGALFK
                     ANGIDLYKGKASIPEAGVVEVNGEKLTAKNTIIATGGRPAQLPGLEFDGTRVIGSTDA
                     LNLERIPESVVVIGAGALGTEFACIWNAFGAKVTLVEMMPTILPRSDSEPAKLLERIL
                     RKKKIDVRTGTRVEKIERKEDGVTVHFAGGKADSVDAEIVLVAIGLQCNSEIVANSPA
                     LNIPLGKRGGIPVNDRMESPVPGIYAIGDVIDRTWLAHGASAEGIVAATNCTGGNAVM
                     DYRVLPACNFSSPELASVGLTEQEAKDQGYTVKTGSFLFAANGRAHTLGSAEGMVKII
                     GDAATDEILGVHILGPEAGEMIAVGALAMRVEGTVEEIASTIHTHPTLSEALWEAAED
                     YYGRGIHSKPKK"
     gene            4557..6083
                     /locus_tag="PKN92_04505"
     CDS             4557..6083
                     /locus_tag="PKN92_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48086.1"
                     /translation="MDAEAKSTLENMSAVLDELDAQRAHSVAAIVEGLEKVKLMFVRR
                     GEMLARENASAAEKQQAVFNSELQEVQSKESLLREEISRLTAELADRDALAEKLALLE
                     RQLSDYEEVKQNNQIQEQQIRALEEQLQSRGEIIQEKEAVLAEKEAVLAEKDGELLRN
                     EELLREKEAEIAAVNETLEASRQTCAAVREELDRLKLDYSALGDEHSAIQQEREELHA
                     RLQEREQENQQLAEEIQAAAAAKQMADEIQQALEEKEKHIQSLESALQDAAESSDKYE
                     VQTKMLLDQLENFKQQREEMTELRDQLKQAEAALEKERADSIRLRARVAVAGRLSDTA
                     GDKTGVPLSARQVYESVSSATQEKRPSSTRASQRKLFGEILCDAGVVTSEQLEEALRF
                     KASGFKRRIGSVFIELGYATSEVIAAALAAQLSLPFVEKLETMATTDAIRLVPAYLVK
                     NHRCLPISKDGKRLRVAMENPLDLIAIEDIELATGCTVEPAVATSDAITSAIERFYAV
                     "
     gene            6147..7190
                     /locus_tag="PKN92_04510"
     CDS             6147..7190
                     /locus_tag="PKN92_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48087.1"
                     /translation="MLTNLLAFLVLAATQIPADFSGSSPAVENVFGKVPDFFQRYDLI
                     LQPDVLEEGWWAGAPSLMRDSEGTFWMACRMRNTTDPRGLRGYEIRILKSPDGIHFEK
                     ALSIAREAVPIPGFERPALLRDTKTGNAKLYACGPWQGGPWSVIKFDDAPAPDQFCAA
                     SARPVITPFAKSYDHDVVPTEYKDPVILFAENAYHCYVIGYLRKNERIYHFSSPDGEK
                     WEPVGSPYESLMQLQGWHDFFVRPASVLSLGIGYLFVYEGSNVSWYEPVYNIGTGLGF
                     SFDLHNIQDLTPQSPLLLSTTPSAAGHTWRYSSWLWVDGELWVYAEVAAESGIHEIRR
                     FAVPVCKDNGVAR"
     gene            7449..7976
                     /gene="rplJ"
                     /locus_tag="PKN92_04515"
     CDS             7449..7976
                     /gene="rplJ"
                     /locus_tag="PKN92_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNZ48088.1"
                     /translation="MPTEQKIKAVAEIKERVSGSSISIIAQFVGMNVEQATQLRKQLR
                     EAGAQLKVYKNNLAKIALDDLGLSSAAAFMEGPTAWAFCDDPVAPAKVLKTFSKEVQF
                     IAMRGGVLTGAVVNAQQLEALASLPPREVLLAQVVGTIAAPLRNTVGVLSALPRNLVN
                     VLDQIRKQKEETPAA"
     gene            8053..8439
                     /gene="rplL"
                     /locus_tag="PKN92_04520"
     CDS             8053..8439
                     /gene="rplL"
                     /locus_tag="PKN92_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNZ48089.1"
                     /translation="MSEKLETIINSIAELTVLELSELVSALEEKFGVTAAAPMMMGAM
                     PAMGDAAPAAEEPTEFNVILKDFGSQKIGVIKVVKDLLGLGLKEAKDLVDSAPATVKE
                     KAAKEEADKLRAALEEAGATVEIQSA"
     gene            8715..10217
                     /locus_tag="PKN92_04525"
     CDS             8715..10217
                     /locus_tag="PKN92_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNZ48090.1"
                     /translation="MAEIVLGVGYKKPVRVIVRLFDEQITKREGIPGRIRAWTNSSSI
                     VALRSGLEGDFTHYEIRVTKARTDEEALKKYLEDYPDLVCAEEGEDQWCIQVPPSDKE
                     LKEEHHEAFDALTKIIRLPEVWHAEGSDFQDKIINVEKLFQAMIQYKASDVHLTAGLK
                     PIFRIDNDARFSDIMQSFSGSQIRTLIRRTAPPGFYDEFEEFKQTSYSYHQAGMGYSR
                     VSAFLKNGAPHCTFRYLPEKIPSFEDLNVPAQQMQTIASTPRGLILITGMTGSGKTTT
                     MAALLDWINAHKALHILTIENPVEFVHANKKSIVSQRNIGTDVRSFSEAVTGALRHDP
                     DVILIGEMRDPDTIRAAINAAATGHLVISTLHSNTSYEVINRVVSFFDPIERDLVRLQ
                     LRDCLRGVICQRLVPKVGGGRVPALEFLFNDIKPINDAILKGDTDAIRVGMQQTISHS
                     MLFEQYLLKMFKEGVVKLEDAREMSTDVSVFDQMRMGTYAVPRLDSIKGG"
     gene            10389..11330
                     /locus_tag="PKN92_04530"
     CDS             10389..11330
                     /locus_tag="PKN92_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ48091.1"
                     /translation="MKNQYISMLHDGDRVNDYFLAMRKDLRDRSNGGKFLGSSLKDKT
                     GEIGAVMWSNAVEAAAAFEIGDVVHVRGVVGTHQGRLQVRMEQILPLRKEDYSLSDLL
                     YEPENTGAVEESYGKTLGSISNPHCKALVRSFWDNESVRKSFCRASAGKKWHHEFVGG
                     LLHHCHEMSCLAETMCTILPELNRDLLLTVIFLHDIGKLKELAHGFAVDYTTQGKLLG
                     HIYMGCEMVQQKMKEIPDFPDRLKMEILHCLLAHHGTLESGSPVKPCSLEALVLHHID
                     DLNAQSAAFSRIIRESRSKAQEWSDYLPIIDRAVWAG"
     gene            complement(11340..11879)
                     /locus_tag="PKN92_04535"
     CDS             complement(11340..11879)
                     /locus_tag="PKN92_04535"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ48092.1"
                     /translation="MEIWKNSELVFVGKVVALRTGEVRLDDGSEAYREVVEHPGGVCI
                     LPFTGQAFVLVKQFRIALGKDMIEAPAGKLEVNETPLQCAVKEMHEETGYRADRLISL
                     GVIHPSVGFCSEAIHLFLALDLTEIGQKLEKDERIEMLHVPLEEASALACSESLQDAK
                     TVVILNRALAWLSARKKEI"
     gene            complement(11993..13237)
                     /gene="rho"
                     /locus_tag="PKN92_04540"
     CDS             complement(11993..13237)
                     /gene="rho"
                     /locus_tag="PKN92_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNZ48093.1"
                     /translation="MKDMKSMTMQQLNDLAEELGLTEYGGLKRQDLIFRLLQASIEKA
                     GSAYGEGTLEILVDGFGFLRSPDYSYLPGPDDIYVSPSQIRKFGLRTGDSVSGHVRPP
                     KSGERYFALLKVESVNHESPEDAYKRINFDNLTPLHPNERLRLETTQDETAMRIMDLI
                     SPIGKGQRGLIVAAPFTGKTVLLQKIANSLAVNHPETTVIVLLIDERPEEVTDMQRSV
                     QGEVIASTFDEPPERHIQVAEMVLNKAKRLVEHKRDVIILLDSITRLARAYNVIVPAS
                     GKILSGGVDSNALQRPKRFFGAARNIEEGGSLTILATALIETGSRMDDVIFEEFKGTG
                     NMELHLDRRLMEKRIFPAIDVNKSRTRKEELLISAEELERIWLLFRVLSPLESTEAAD
                     LLIGKLKKTKSNEDFLSHMQKM"
     gene            complement(13591..16305)
                     /gene="polA"
                     /locus_tag="PKN92_04545"
     CDS             complement(13591..16305)
                     /gene="polA"
                     /locus_tag="PKN92_04545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNZ48094.1"
                     /translation="MNQTFYLIDAMAFIFRAFHAINANLTDANHRPTNAVYGFTRILL
                     RILREQRPDYIAVVFDSREKNFREEMFPEYKANRKATPPELIEQFPRIYDVVEALNLP
                     ALTVPGVEADDVIGTLAVQAEKAGMDVVLVSGDKDLLQLIGKQVRMFDPGKDESKAWW
                     GPDDVKMRFGVTPEFVPQALALIGDTADNVPGVRGIGEKTAAKILSQYQTLENLYAHL
                     DEFKGKQRENLEADKEQAFFARKLVTLRTDVALQEDLETFKRKEWKQEDVEACFRALS
                     FHSILEELFSKEKNVPAAVQHQYHLVMTRSELQQTVDALKKAGAFALDTETTSIHPML
                     ARLAGISVCAEAGRAWYIPLGHEAPDLDALMAGVDFSAPEFSTEEALAALKPLLEDAQ
                     YKKFGHNIKYDMIVLARAGIHLQGIAMDTMLASYLTDASRLRHNLDELSLLYLNHKMI
                     PITDLIGKGAHSIPFTQVPPGPASEYACEDADMTLRLERRLRPDLIQEDLLPLLDNIE
                     LPLISVLASMEMAGIALDTSQFTALQQELAVKLKKLEYTIYDLAGESFNINSPKQLQY
                     ILFDQLRLKPGRKIKSGYSTDLETLESLAQEHPLPASLVEYRGLEKLRSTYVDVLPKL
                     VNPETGRIHTSFNQAVAATGRLSSSNPNLQNIPTRTELGRRIRSGFVAGDKKKRLIAA
                     DYSQIELRILAHLAKDDALCEAFRSDKDIHRDTASRIFQVPPEEVTADMRRQAKAINF
                     GVIYGMGEVSLAKNQGLTRQEAKSFIDSYFNTYPGVTAWLEKTRQEARKNGYVVTLMN
                     RKRILPDIQSASPMLRSAAERMAVNTPVQGSAADIIKIAMIRLHEALKPLKAQLLLQV
                     HDELVIEADEAIAEETAELTRSIMEDAMALLVPLKVDVGIGNHWAEIH"
     gene            complement(16462..17955)
                     /locus_tag="PKN92_04550"
     CDS             complement(16462..17955)
                     /locus_tag="PKN92_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNZ48095.1"
                     /translation="MLIGSQTTSTLLDVFRIGMTEIIRATGLPTEAEFKTHNCTISVQ
                     GKNLSNRIDTMRIYFTPREGVAPSEVENALNATRLGFRMRQENGQLVISVPSFEWRQL
                     PALIPLDQIIEQFVSKSLRSETWELEVSEYVDNGFNINRLIEIMKEKKASDLHLRAGN
                     RPFIRVDNDLLPLEDTPVISKDDMEKFVLQLGGEAELQMLERERESSFQYHAAGIGYL
                     RCSGYIKSGAMAIALRLIPEQPLPFDALNIPDVVRKVCDKHRGLFLVCGITGSGKSTT
                     LAAMIDYINEQRRAHIITIEDPVEYVYVDKKSIVSQRQVGRDTHSFGNALRGSLREDP
                     DVILVGEMRDMETIRAGLSAAETGHLVFSTLHTTTAVDTINRMISYFPQNERDLIRQE
                     IAYSLQGVVCQRLLKKIGGGRIPAVEILLGGMPIVRDAILDGDLDKLHGIIENDSDMR
                     SFDQYAVELYQKGLVSKEEAISACANEEGFQRIISGIKSSEGRRLLK"
     gene            18277..19065
                     /locus_tag="PKN92_04555"
     CDS             18277..19065
                     /locus_tag="PKN92_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNZ48096.1"
                     /translation="MSERAFIETFLRANSDYVTQKYQEKQNVTVTSKDSPVNLLTEVD
                     LTIQKRFIDATAEVWPHDLVVGEESGHDRYPADPNGRIWVIDPIDGTYNFVRGLNPSF
                     AISIAFMQDGLVQAAGVSMPLAGYYFFAEAGGGSFCNGRRLSVSRVQHLESACVEVDF
                     SSVKNRRTILRRGAKIIREAAQLRSHGSAVAAICQIATGDADAYVHPGLHPWDYAAAQ
                     LIAEEAGALATRLDGQPLRVFDKKKGVLVTNGALHEQLLASLTR"
     gene            19103..19546
                     /locus_tag="PKN92_04560"
     CDS             19103..19546
                     /locus_tag="PKN92_04560"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNZ48097.1"
                     /translation="MDLLREYFKGDKLAATLGMTIERIEPGAAVVTMPLAECHQNGLG
                     SAHGGALFSLADFCFAVASNSHGRVAVAINCTISFHRGIRSGVLTATAREIHCGATIA
                     AYIVEITDEQHKSIATFQGQVFRTQNTIEAVLAERKAAGQEKDPD"
     gene            complement(19547..19921)
                     /locus_tag="PKN92_04565"
     CDS             complement(19547..19921)
                     /locus_tag="PKN92_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HNZ48098.1"
                     /translation="MRKHIIGSAVILSFAVALLFAASAQAQLYNPDVDLPKPTGMEKS
                     LTKMGRGISNILFGWAEVPVTFDRNLKKGKPLGYLVGVAPVLGSARAVMRTGTGMFET
                     LTFPFSDKDVNFEPVLEPEFLF"
     gene            complement(20046..21242)
                     /locus_tag="PKN92_04570"
     CDS             complement(20046..21242)
                     /locus_tag="PKN92_04570"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ48099.1"
                     /translation="MTTSTLALLGGKAVRHPDKNWPAWPQYDQREENALLEVLHRDSW
                     LFGPKVQEFEAAYAAFQGADHCISCNSGTAAAEIILQAMGIGAGDEVLVPPYTFIATA
                     SAVLRVGATPIFVDLDDSWCMDPDLAEKAITPRTRAIMPVHFGGRICDMDKMNELGSR
                     HGIPVIEDACHSWGGRWKSQGTGTIGACGFFSFQASKNITAGEGGGIVTNDEELATKV
                     RSLTNCGRGKPGSPWYYHLNVGTNARLSEFQAAILVCQLERAEEHILRRAENASILNQ
                     GLAEIEGLHLQRKSNRITRRAWHLYCLRLDESAFGCTRERFVEAAVAEGLPITAGYPM
                     PIYKQPVFQNLTCYDYSTVSCPFTEDLCAKSGMWLPHQILLGTEEDMHDIVSIIRKIK
                     ENASSL"
     gene            21512..22837
                     /locus_tag="PKN92_04575"
     CDS             21512..22837
                     /locus_tag="PKN92_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNZ48100.1"
                     /translation="MNSPYDILHHSLSQKSALVAIIGLGYVGMPLATYFHRSGFRVLG
                     VDLDEEKLAALRSGRSYLNHLPADWIPAALEAETLFLTSDYSALRDADAILICVPTPL
                     GKHREPDLSHIIHATSQIACFFKRGTLVSLESTTYPGTTTEVVLPLLSKGGRVAGKDF
                     FLVFSPEREDPGNKSHALSSIPKVIGGVTPQCAALAQLLYGAIFTETVPVSQPAAAEL
                     CKLLENIFRSVNIALVNELKMLCGRMGLDIWEIIDAAATKPFGFMPFYPGPGLGGHCI
                     PIDPFYLTWKAREYGFATRFIELSGEINTSMPQYVLSRCMQALNMTGRPLRGSRVLVL
                     GAAYKKDINDLRESPSIPLMNLLEENGAIVQYNDPFIPHIAAMGANSPERNSIELTSA
                     ALKDSDLVLIATAHSCYDPAFIVEHAPLVVDTRNMTGKLLENRHKIVQA"
     gene            complement(22853..24031)
                     /locus_tag="PKN92_04580"
     CDS             complement(22853..24031)
                     /locus_tag="PKN92_04580"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNZ48101.1"
                     /translation="MRNVVIASAVRTAIGSFGGTLKDIPASDLMGLIVKEAVSRANVP
                     PELVGDVIVGQCMQRADESTSGRLAALKAGLPIEVPGLTIHRNCASGMQSIIYGAQQI
                     MLGDLDVVVAGGVEVMSRVPYTLKDARWGKRLQHGVMSDGIWDGLTDPYCGLIMGLTA
                     ENLAEKYDISREEQDVIALRSHNNAEAATKSGRFAEEILPVAIPSRKGEPVQFKEDEH
                     FRPGMTMDQLARLKPSFKQDGTVTAGNASGINDAAAAVVLVAEERVKDLGVTPIARLK
                     GYGMAGVEPELMGYGPVPATKQALSRTGLSLDDIELIELNEAFAAQYLACEKLLCLNR
                     DITNVNGSGIGLGHPVGCTGARIIVSLLHEMKRRGSSLGLATLCVGGGIGQASIWEMC
                     "
     gene            complement(24279..25550)
                     /locus_tag="PKN92_04585"
     CDS             complement(24279..25550)
                     /locus_tag="PKN92_04585"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNZ48102.1"
                     /translation="MSIDAVVGANWGDEGKGRMVDYLARDAAFVVRYQGGNNAGHTVV
                     NDLGEFKLHLLPSGVFYPGVTNILGPGMVIDLEGLCDELDDLASKNIQPVIRISDRAT
                     ICFPFHRIEDGLEEARLGKDAYGSTLRGIAPAYGDRTVKKALQIGELLHPERFEKRLR
                     QISNWKLERLEAFYGKKDIFSVDEMIEWAKKYGDRLREYVTDTTVLLEAAAKEGKNIL
                     FEAQLGTLRDLNFGIYPFTTSSCTLASYAPIGSGLMGYPLDTVLAVVKAFSTCVGEGP
                     FVALMDKEEGDALRISAHEFGASTGRPRTIGHFDAVATRYGVKMQGATEVALTKLDCL
                     SGKKTLKICTRYAWQGKEYADFPLNAVLEEATPVYMEVPGWDEDVTQVRRYEDLPEAA
                     RNYVEEIEKLIECPLRYVSVGPEREALIVRN"
     gene            complement(25759..26367)
                     /gene="rpsD"
                     /locus_tag="PKN92_04590"
     CDS             complement(25759..26367)
                     /gene="rpsD"
                     /locus_tag="PKN92_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNZ48103.1"
                     /translation="MARYHGPKNRLSRRVGKCIWGRSNKVVEKRPYPPGQHGKNLRRK
                     LSVYGVQLLEKQKMRMYYGGIMERQMRRIFDNARRLGGNTGTNLMMLLESRLDTVVWR
                     LGFTNTIFAARQLVNHCHVQVDGKKVNIPSFQVKPGMVISIRARSRKIPMVVDGVNNP
                     PYEIPEYLQRDAKSFEGKMTATPNVPFPVEFDTTSVIGFYSR"
     gene            complement(26557..26633)
                     /locus_tag="PKN92_04595"
     tRNA            complement(26557..26633)
                     /locus_tag="PKN92_04595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26597..26599),aa:Arg,seq:acg)
BASE COUNT         6344 a         6725 c         7228 g         6463 t
ORIGIN      
        1 ttagaaatag catctcgcgt taagattcct gcctgcgcag gaatgacgcc gggggggggg
       61 acggcggaag ggaaaaatgg caggggctgt tgtggtctgt gtttttggga ggtatgtcgc
      121 aggacgtatt tccgatacag attcgagtga ctgtcttcgc cctgacccaa ttttgtgcca
      181 atcgacctat tggcaggagg cttttatgtt ctgtgattat ggaggtatgt cgcaggacgt
      241 atttccgcag cgaatctgag tggctgtctt cctcatggcc cgcttgtgtg ccaataggtc
      301 gttggctttc ccagggagcg ctgcttttag tcttttgtat tgaacaggct gtttccctgt
      361 gtcccggaga aattgctata ctaagatttg tagaagcgct ttggcagtga gggttgaact
      421 gcatctgcag tcaccctttt tcatgctggt atatgttccg atttccccag tacaaggata
      481 gtatgttgct aaaaagggca gtccatttcg gtgccggtaa tattggccgg ggttttctgg
      541 gacagctgta ctgtgaaagc ggctatgcca ccgtgtttat cgatgtggag tccgaagtgg
      601 tggatgcgct caatagccgg ggcagctaca cggtcgacct ggtttcggac gaagggacgg
      661 aaacacgcac gataagcgct gtgagtgctg tacatgcttc tgatccggat aaagctgccg
      721 taacgctcgc acaggcctct attgcctcta cagccgtggg catgcatgca ctgcctcata
      781 ttgcgcctgt aattgccgct tcaatacctc tccgcttttc cgatccacat gcgcagcctt
      841 tggacatcat tctatgcgaa aatatacatg acggtgaggc gcatatgcgt gcactggtcc
      901 gcaagcatct gcctgcggac tggcatcagg ttcttgacga aaaagtaggc tttgtcgaag
      961 cgagtatcgg ccggatggtg ccgacccgca cccgtgccca gcaggaagcg tatattcttg
     1021 ctgttgcggt ggagccttat tgcgaactgc ccgtggatgc gcatgctttt cgaggcgccc
     1081 ttcctcctgt aaagcatctg ctaccgaccg ccaacttcaa aggatatgtg gagcggaaac
     1141 tttttgttca taacctgacc catgcagcaa cggcttatct gggctacagg cgcggctata
     1201 cctatatctc ggaagcgatc cgtgatgcgg aagttcgaaa atctgtggaa ggtgccgggc
     1261 tggaaagctg cagggcactg gtgcaaaagc acggattgga tgctgaatcg ctggaaagcc
     1321 accgtcagga tctgatccgt cgctatcaca accgtgcgct ggctgatcag gtggaaaggg
     1381 ttgcccggga tccgcggaga aagctggggc gggatgaccg gcttatcggt gccatgcttc
     1441 tttgtctgga tcaggggatt gtgcccgatt ctattgcatc cgttaccgcc gcagcactga
     1501 cctatgacaa tccggtcgat cctgtcgctc tggaactgca gaaatatctg cgtgcttccg
     1561 ggttggatgc cgtcctgaaa gacatctgcc gtatcgatcc cggttccgat gctgccgggc
     1621 tgattaaaaa atactataat aaaaagcaat ccaccaactg aaggataccg tacttatgca
     1681 tgaaatctta cttccgaaaa tgggtaattc cgttgaagag gcggagattg tgaaatggtt
     1741 caaaaaagag ggggatgcag tggaaacagg cgatccgctc ttttccattc agacagacaa
     1801 ggcagaagtg gaatgcgaaa gtaccgcttc cggcattctg cgcaaaatcc tggttccgga
     1861 aggggtcgag gtgccggtgc tgactgtagt tgcgctggtg ggcgatgccg atgaagcatt
     1921 gcccgacaac atgggcgcgc cttctgcatc ggcatcggcg cctgcagcac aggtttccgc
     1981 accgataact ggccctgctg cagcggctcc ggctgcgcct gtttcgcccg ggacaggtgc
     2041 cacaggaaaa tcacccgtat ccccccgggc acggaaagcc gcagaggcac ttggcattaa
     2101 tcctgccacg ctgtcgggtt ccggcgtggg cggtcgggtg atggaacagg atgtactggc
     2161 gtcagcccgc agcggaggcg cagtcaagat tacgcccacc gcacgacgca tggcagaaaa
     2221 tgccgggctt tctgtagcgc agctgagcgg taccggcatc ggcggtaaaa tcacaaaagc
     2281 cgatattgcc cgttcgctgg agcagggcat cgctcaggca gctccggaag cgaaggaggg
     2341 cattcgtact gttcctttat cacccatgcg ccgcattatt gccgaccgta tgtgcgcaag
     2401 caaatattca gcgcctcact actatattac tgtagaagtg gatatgagcg ccgccaaaaa
     2461 tttccgcgcg gcaaaccgcg gcttcaaggt ctcttataac gatctggtac tttttgcgac
     2521 ggcgcgtgct ttgcgcagct tcccggctgt caatgcccgc tggtgcggcg attttatcga
     2581 agaattgagt gaggtgaatc tcggaatggc cgttgctctg ccgacagggc ttattgttcc
     2641 ggtcatccgc aatgcccacc tcctgtcttt ggaggggctg tcttcgacag cgaaagcact
     2701 ggcggaaaaa gcgcagacag gcaagctgct gccggatgat tatcagggca acagttttac
     2761 cgtatctaat ctgggagctt tcggtgtgga tcagtttacc gccattatca accagcccga
     2821 cagtgccatc cttgccgtag gccagatgaa ggacagggca gttgtgattg acggcggtat
     2881 ccatattcgc cctgtaatga agctgaccct ctccagcgat caccgggtga tcgacggagc
     2941 tgtggcggca cagtttatga gtcatttgaa atccatcctc gaagagggcg ctttttaaga
     3001 aagcagtccg ggagcctatt tatgaaaaat tatgatgttg ttgttatcgg tgccggtccc
     3061 ggcgggtatg tcgcagccat tcgagccgct cagcgcggtg ccagaacagc ggtggttgaa
     3121 tataggaatc tgggaggcgt gtgcctgaac gaagggtgca tcccgacgaa aacgctcatt
     3181 catacagccg aactttacag aaagctgcag aatgctgcag agttcggcat agaagtgcgt
     3241 gatgtgcggg tgaatgtccg gaaaatgctt gatcgcaagg aaacagtgat cggcatcaat
     3301 accggcggca tcggcgccct tttcaaggcc aatggcatag atctttacaa ggggaaagcc
     3361 tccattcccg aggcgggcgt tgtggaagta aatggcgaaa aactcaccgc caaaaacacg
     3421 attatcgcaa ccggggggcg tccggcacag ctgccgggtc tggaatttga cggaacccgg
     3481 gtcatcggca gtacggatgc gttgaatctg gagcgtatcc cggaaagtgt tgttgtgata
     3541 ggcgccggcg cactcggcac tgaattcgcc tgcatctgga atgctttcgg tgccaaagtc
     3601 acccttgtgg aaatgatgcc caccattctg ccccgaagcg attccgagcc tgcaaaactg
     3661 ctggagcgca ttttaaggaa gaagaaaatt gacgtgcgca ccggcacccg ggttgaaaag
     3721 atagagagga aggaagacgg cgttacagtt cattttgcag gtggcaaggc tgatagcgtc
     3781 gatgcggaaa tcgtgctggt ggcgataggg cttcagtgta attccgagat tgttgccaac
     3841 agccccgcat tgaatattcc gctggggaaa cgaggcggaa ttcctgtgaa cgatcggatg
     3901 gaaagtccag tgccgggcat ttatgccatc ggcgatgtga ttgaccgcac ctggctggcc
     3961 catggtgctt cggcggaagg tattgttgcg gcgacaaact gcaccggcgg aaatgcggtg
     4021 atggattatc gggtgcttcc ggcctgcaat ttcagttcgc cggagcttgc gagtgtcgga
     4081 ctcaccgagc aggaggcgaa agatcagggt tatacggtaa aaacgggcag ttttcttttt
     4141 gccgctaacg ggcgtgccca taccctcggc agcgctgaag gcatggtgaa aataatcggc
     4201 gatgctgcca cggacgaaat tctgggcgta cacattctcg gtcctgaagc cggagaaatg
     4261 attgctgtcg gcgcacttgc catgcgcgtg gaaggcacag tggaggagat agcctctaca
     4321 atccacaccc atccgacctt gagcgaagcg ctctgggagg ctgctgaaga ctattatgga
     4381 agaggcatac actcaaaacc taaaaaataa atagcacata tcagccgaaa aaagatcaac
     4441 gttcttgcag attatttcgc ctgtcgttat aatgcggtat gtcgtgtaag cccccggttt
     4501 tttcacttcc actgaaaaag tgaaggtttg tgctccggca tgtaaaggaa agatttatgg
     4561 atgctgaggc taaaagcact cttgaaaaca tgagtgctgt gctggacgaa ttggatgcac
     4621 agcgtgccca ttctgtagcc gcaattgtag aaggacttga aaaagtcaaa ctcatgtttg
     4681 tgcggcgcgg agaaatgctt gcccgggaaa atgcatctgc tgcggagaag cagcaggctg
     4741 tttttaattc agagctgcag gaagtgcagt ctaaagaatc actgctgcga gaagaaatca
     4801 gccggctgac agccgaactg gctgatcggg atgcgttggc ggaaaaactg gcattactgg
     4861 aacgtcagct ctctgattat gaagaagtta aacaaaataa ccagattcaa gagcagcaga
     4921 tacgtgcatt ggaagagcag ctgcagagcc ggggtgaaat aattcaggaa aaagaagcag
     4981 tgcttgcgga gaaggaggct gttcttgcgg agaaagatgg ggagctgctg agaaatgaag
     5041 agcttttacg ggaaaaagag gcggaaattg ccgctgtgaa tgaaacactg gaagcgtccc
     5101 gccagacatg tgcagcagtc cgcgaggaat tagatcggct taaattggat tacagtgcac
     5161 tgggagacga gcacagtgcg atacagcagg agcgggaaga gttgcatgcc cggctgcagg
     5221 aaagagagca ggagaatcaa cagctggccg aagaaattca ggcagctgcc gcagcaaagc
     5281 aaatggctga tgaaatccag caggcactgg aagagaaaga aaagcacatt caaagtctgg
     5341 agtctgcttt gcaggatgcg gcggaaagca gtgataaata cgaagtgcag acaaaaatgc
     5401 tgctggatca gctggagaat ttcaagcagc agcgcgaaga aatgactgag ctgcgtgacc
     5461 agttgaagca ggcggaagcg gcattggaga aagagcgggc ggatagcatc cggctgcggg
     5521 cacgtgtggc agtggcaggg cgtctttcgg acaccgccgg cgacaaaacc ggtgtgcccc
     5581 tatctgcccg gcaagtatat gagtctgtgt cttcagcgac acaggaaaag cgcccatcgt
     5641 cgacaagagc aagtcagcgt aaacttttcg gtgagattct gtgtgatgct ggtgttgtta
     5701 ccagcgagca gctggaagag gcgttgcgtt ttaaagcgtc agggtttaaa cggcgtatcg
     5761 gcagcgtttt tattgaactg ggctatgcca catctgaagt gattgccgcg gcacttgccg
     5821 cccagctgtc cttgcctttt gttgaaaaac tggagacgat ggcaaccaca gatgctatcc
     5881 gcctggttcc cgcatatctt gttaaaaatc accgatgtct tccaatctca aaagacggaa
     5941 agcgccttcg ggttgccatg gaaaaccctc ttgacctgat tgccatcgag gatattgaac
     6001 ttgcaacggg gtgcactgta gaaccggcag tcgctaccag tgatgccatt acatcggcca
     6061 tagagcgttt ttacgctgtg taatttcgtg cggaaaaggg gaagtgcccg aaagccttcc
     6121 tggcatttta gaaggaattc tttgccatgt tgacaaatct gcttgctttc ctggtgttgg
     6181 cagcaacgca gatacctgcc gatttttccg gaagttcacc tgctgtcgag aatgtttttg
     6241 gtaaagttcc tgactttttt cagcgctatg acctgatcct tcagccggat gtgcttgagg
     6301 aaggctggtg ggccggggca ccatccctga tgcgtgacag cgagggtacg ttttggatgg
     6361 cgtgcaggat gcgcaatacc acggatccgc ggggactgag aggctatgaa atccgcattc
     6421 tgaaaagccc ggacggaatc cattttgaaa aagccttgag tattgcgcgg gaagctgttc
     6481 ctatccccgg ctttgaacgt cctgcactgc tccgggatac gaagaccggc aatgcgaaac
     6541 tgtatgcctg cggaccctgg cagggcgggc cctggtctgt tatcaaattc gatgatgcac
     6601 ctgctcccga tcagttttgt gcagcgagtg cacgcccggt catcacgccc tttgccaaaa
     6661 gctatgacca tgatgttgtg ccgactgagt ataaagatcc ggtaatactc tttgcagaaa
     6721 atgcctatca ctgctatgtc atcgggtatc ttcgcaaaaa cgagcgtatt taccattttt
     6781 caagcccgga cggagaaaaa tgggagcctg tcggcagtcc ctatgaatcc cttatgcagc
     6841 tgcagggctg gcatgatttt tttgtcaggc ctgcttccgt gctctctctg ggaataggct
     6901 acctttttgt gtatgaaggc tccaacgtga gctggtatga gcctgtgtac aatatcggca
     6961 caggactggg cttcagcttc gatctgcaca acatacagga tcttacacct cagtcaccgc
     7021 tccttttaag cacaacgcct tctgccgccg ggcacacctg gcgttattca agctggcttt
     7081 gggtggacgg tgaactatgg gtctatgcag aagttgcggc ggaatccggc atccatgaga
     7141 tccggcgttt tgcggtgccc gtctgtaagg ataacggtgt tgcccgctga caggcatagc
     7201 cctgaaagga catgaagttg cgcacttact gcgttttttg ctagaatatg gtcagactat
     7261 gtgcaatcgc actgctggtc agagacagcc ggtggcatta gtgccttaat atcctgccga
     7321 gaccgcggag gaacgcaccc gtattataat tcggatgtat cccgcctgtg ttgtctctct
     7381 gacaatacag gcgttttttc tgcgctgctg ccagacagat gcgaactcgt gaaaggaggt
     7441 gaattctctt gccaacagaa caaaagatca aagccgttgc tgaaattaag gaacgggtca
     7501 gcggtagcag tatctccatt atcgcccagt ttgtagggat gaatgtggag caggcgactc
     7561 agctgcgcaa gcaattgcgt gaagcgggcg ctcagttaaa agtatacaaa aacaatcttg
     7621 ctaaaatcgc tttggacgac ctgggacttt cttccgcagc agccttcatg gaaggcccga
     7681 cagcctgggc attttgtgat gacccggtag cccccgccaa agtgttgaaa actttttcca
     7741 aagaggtgca gtttatcgcc atgcgtggcg gcgtgctgac aggtgccgtc gtcaatgcac
     7801 agcaactgga agcactggct tctcttccgc cgcgcgaggt gctccttgct caggtggtgg
     7861 gaactatcgc cgcacctctg cgcaataccg tcggcgtcct cagtgccctt ccccgcaatc
     7921 tggtcaatgt gctcgatcag atccgcaaac agaaagaaga aaccccggcg gcatgatgcc
     7981 ggacactcaa caacaagtaa accaacactc ttgccggcat gaagtccgga tcagaaacaa
     8041 ggaaacaaaa caatgtccga aaaacttgaa accattatca acagcattgc tgaactgacc
     8101 gttcttgaac tcagcgaact ggtctccgct ctcgaagaaa aattcggcgt gacagctgcc
     8161 gcccccatga tgatgggcgc catgcctgcc atgggtgacg ccgctcccgc agctgaagag
     8221 cccaccgaat tcaacgtgat cctgaaagac ttcggttccc agaaaatcgg cgttatcaag
     8281 gttgtcaaag acctgctggg tctcggtctg aaagaagcca aagacctcgt tgacagcgcc
     8341 cccgcgaccg ttaaagaaaa ggcagccaaa gaagaagcag ataaactgcg tgccgctctt
     8401 gaagaagccg gagccaccgt cgagatccag agcgcctgat ctcgcatatc ctttgtccga
     8461 attttgcacc gcagcattct gttgcggtgc gtttttttta tgatgtaccc aaagtcaaaa
     8521 aaaacagtgt gcccttttta taaaaagatg atccccaatg cccctattgg ttcatgcaaa
     8581 aacgggccgg aagaaaatgc ttgacgtttt cgcaatattc atgtattatg gtgccgtatc
     8641 tcagtagtgt ctgaacttcg tccgcttgtt tctaaggtgc tctttatccg gatgtatgca
     8701 acgggaggtg caggatggct gaaatcgttt taggtgtcgg ttacaagaaa cctgtgcgcg
     8761 tcattgttcg gctctttgat gaacaaatta ccaaaaggga agggattccg gggcgtattc
     8821 gtgcctggac caattcaagc agcattgttg cgctgcggag cggcctcgag ggcgatttta
     8881 cacattacga gatccgcgtg accaaagcgc gcactgatga ggaagcgctg aaaaaatacc
     8941 tggaagacta tcccgattta gtctgtgcgg aagaagggga ggatcagtgg tgcatccagg
     9001 tgccgccttc ggacaaagag ttaaaggaag agcaccacga ggcatttgat gcgcttacaa
     9061 aaattattcg ccttcccgaa gtgtggcacg cagagggctc agactttcag gataagatca
     9121 tcaatgtaga aaaactcttt caggccatga ttcagtataa agcaagtgac gtgcacctga
     9181 ctgccggcct gaaacctatt ttccgtattg ataatgatgc ccgtttttca gatatcatgc
     9241 agtccttttc cggatcgcag atccgaacgc tgatccgcag gacggcgcct cccggatttt
     9301 atgacgaatt tgaggagttc aagcaaacca gctacagcta tcatcaggca ggcatgggct
     9361 actcccgtgt atctgctttt ctcaaaaacg gtgcaccgca ctgtactttc cggtacctgc
     9421 cggaaaaaat cccttccttc gaagatttga atgttccagc gcagcagatg cagacaattg
     9481 ccagcacgcc gcgtggactt atcctcatta ccggaatgac aggcagcggt aaaaccacta
     9541 ccatggcagc ccttctggat tggattaacg cacacaaggc gcttcatatt ttaacgatag
     9601 aaaaccctgt tgaattcgtt cacgccaaca aaaaatccat tgtttcccag cgaaatatcg
     9661 gaacagatgt gcgctctttc agcgaagcgg tgaccggtgc ccttcgtcat gatcctgatg
     9721 ttattctcat tggtgaaatg cgcgaccccg ataccattcg tgctgcgata aatgctgccg
     9781 ccaccggtca tctggttatc agtacgcttc actccaatac ctcctatgaa gtgatcaatc
     9841 gtgtagtgag cttctttgat ccaatagaac gggatctcgt ccggcttcag ctgcgggact
     9901 gtctgcgcgg tgttatctgt cagcgccttg ttcccaaagt gggaggcggg cgtgtgccgg
     9961 cgctggaatt tctgtttaac gacatcaagc ccattaacga cgcaatttta aaaggggata
    10021 cagacgcgat tcgggtcggc atgcagcaaa ccatttcgca ttccatgctt tttgagcaat
    10081 atctgctgaa aatgttcaaa gagggcgtcg ttaagctcga agatgcccgg gaaatgtcaa
    10141 cggacgtgag tgtttttgac cagatgcgca tgggtactta tgctgtgcct cgcctggact
    10201 ccattaaagg ggggtgattc cctgaaggat gttgtgttag aattggctcg acgaaatatt
    10261 caagccagtg cagggcagat gacgacaaaa atgcatgtgc gccgccgttg agacggcgct
    10321 ttttctgttt gtctgccttt gcaggcactg cctatcctgt aaaggagtgt gggagcacca
    10381 tatttagcat gaaaaatcag tatataagca tgctgcatga cggggatcgg gtcaacgatt
    10441 attttctggc gatgcgcaag gatttaaggg atcggagcaa tggcggaaaa tttctgggga
    10501 gttcgctgaa agacaagacc ggcgagatcg gggcggtcat gtggtcgaat gcggtggagg
    10561 cggctgcagc ctttgagatt ggtgacgtgg tgcatgttcg cggcgtcgtt ggaacgcatc
    10621 agggtcgttt gcaggtgcgc atggagcaga ttttgccact gcgaaaagag gactattctc
    10681 tcagcgacct gctgtatgag ccggaaaaca caggtgccgt tgaggagtct tatggcaaga
    10741 cactgggttc catttccaac ccgcactgca aggcgcttgt tcgcagtttc tgggacaatg
    10801 aatctgtacg caaatctttt tgccgggctt ccgccggaaa gaaatggcat catgagttcg
    10861 tcggcggttt attgcatcat tgccatgaaa tgtcctgtct tgccgaaacg atgtgcacaa
    10921 ttttgcccga attgaaccgt gatctgctgc tcacggttat tttcctgcat gacataggga
    10981 aactcaaaga actggcgcac ggcttcgccg ttgactatac gacccagggg aaattgcttg
    11041 gacatattta tatggggtgt gaaatggttc agcagaaaat gaaagagatt cccgattttc
    11101 cggatcgcct gaaaatggaa atactccatt gtctccttgc gcatcatggc acgctggaaa
    11161 gcgggtctcc tgtaaaaccg tgcagtcttg aggcgctcgt gctgcaccat atagacgatc
    11221 taaacgccca atcggccgct ttttcccgga ttatccggga gagccgcagc aaggctcagg
    11281 aatggtcgga ttatctgccg attattgacc gtgctgtctg ggcagggtaa aagcgtgcct
    11341 tagatttctt tcttccgggc tgaaagccac gccagagcgc ggttgagtat gacaacggtc
    11401 ttggcatcct gcaggctttc gctgcatgcc aatgcagaag cctcttccag tggcacgtgc
    11461 agcatttcaa tacgttcgtc tttctccagt ttctgtccga tttctgtcag atccagtgca
    11521 agaaaaagat gaattgcctc gctgcagaac ccgacagaag gatggatcac tccgaggctg
    11581 atcaggcggt cagcccgata tcctgtttct tcgtgcatct ccttcacagc gcattgcagc
    11641 ggcgtttcat tgacttccag cttccccgca ggtgcttcaa tcatatcttt tcccagtgcg
    11701 atacggaatt gctttaccag aacaaaggcc tgtcctgtaa aaggaagaat gcacacgccc
    11761 cccggatgct ccaccacttc ccggtatgct tccgagccgt catccaggcg cacctctccg
    11821 gttctgagag ccaccacctt gcctacaaaa acaagttcgc tatttttcca gatttccatg
    11881 aatgcgatac tttcctttgg cggatgtgtc ctgttacaaa tcgaaaaaag ggagtggaac
    11941 catctgcacg gtgccactcc cctggttgcc agaatgagga agcgaaaggg ctttacattt
    12001 tctgcatatg agaaagaaag tcttcgtttg attttgtctt tttgagcttt ccaatcagga
    12061 gatccgcagc ttccgtggat tccagtggac tgaggacgcg gaagagaagc cagatgcgtt
    12121 ccagttcctc ggcagaaatg agcagttcct cttttcgtgt ccgggacttg tttacatcaa
    12181 tggcggggaa aatgcgtttt tccatgaggc gccggtccag atggagttcc atattgcctg
    12241 tgcctttgaa ttcttcaaaa atcacatcgt ccatgcggct gcctgtttca atcagggctg
    12301 tggcaagaat ggtaaggctg ccgccttctt caatgtttcg ggctgcgccg aagaagcgtt
    12361 tgggccgctg aagtgcattg gaatctacac cgcctgagag aattttgccg ctggcaggca
    12421 caattacgtt ataggcgcgg gcaaggcggg taatggaatc gagaagaata atcacatccc
    12481 gtttatgctc aaccagtcgc tttgccttat tgagaaccat ttccgctact tgaatgtggc
    12541 gttccggtgg ctcatcaaat gttgaggcaa tcacttcgcc ctgtacagac cgctgcatgt
    12601 ctgtaacttc ttccggccgt tcatcgataa gcagcacaat gactgtggtt tccgggtgat
    12661 tgactgccag gctgtttgcg attttctgca gcagtaccgt tttcccggtg aagggcgctg
    12721 cgacgatcag tccgcgctgt ccttttccga tgggggagat gagatccatg attctcatgg
    12781 cggtctcatc ctgtgtggtt tcgaggcgca gacgctcatt gggatgaaga ggagtcaggt
    12841 tatcaaagtt gatgcgttta tacgcatctt ccgggctttc atgattgact gattccacct
    12901 tcagcagcgc gaagtagcgc tcaccgcttt tgggcggacg cacatgaccg ctgacactgt
    12961 cccctgttcg cagtccgaat ttccggatct gggacggcga gacataaata tcatccggcc
    13021 ctggcagata ggaataatcg ggtgagcgaa ggaagccgaa accgtctacc agtatttcca
    13081 gggtaccttc tccgtaggcg ctgccggctt tttcgatgct cgcctgcagc aggcggaaga
    13141 tcaggtcctg acgtttgagt ccgccgtatt ctgtgagccc cagttcttcc gcaagatcat
    13201 tgagctgctg cattgtcatg gacttcatgt ctttcatggc aatgctcgga ccgtcgctca
    13261 gatcgggcat gggctcattt gccacatcca gaatggcggg tcttctcggc ggctgattgc
    13321 gccttggctt gtttaatttc gactgaccgc tacgcgggtt ggggttgccc ggccgctgtg
    13381 aagggcccgg tctgtgtccg cggggtttgt cctgggagga gtgatccgtc attgacgatc
    13441 ctttcataga cttcgattcg gaatagcggc gttcccgggg tcgggaggga tccgcagagg
    13501 aggtgggttt gtctgacaag ttgctgtttc ccttcaatta aatataaatg aatactgata
    13561 atgactgttt ttagaacaga gaaagctctt tcagtgtatt tccgcccagt gattgcctat
    13621 gccgacatct accttcagag gaaccagcag ggccatcgca tcctccatga ttgagcgggt
    13681 gagctctgct gtttcttccg ctatggcttc gtccgcttct atgaccagtt cgtcgtgcac
    13741 ctgcagaagc agctgcgcct tcaacggttt caacgcctca tgcaggcgga tcatggcaat
    13801 tttaatgata tctgccgcag atccctgcac cggtgtattg accgccatgc gctctgccgc
    13861 acttcgcagc atggggctgg cgctttgtat atccggaaga attcgctttc gattcatcaa
    13921 agttacaacg taaccgtttt tgcgcgcttc ctgtctggtt ttttccagcc atgcggtaac
    13981 acccggatag gtgttgaaat aactgtcgat aaaacttttt gcttcctgcc gcgtcagtcc
    14041 ctggttcttt gcaaggctca cttcgcccat gccgtaaatc acaccgaagt tgatcgcttt
    14101 tgcctgtcgg cgcatatccg ccgtaacttc ctcaggcggc acctgaaaga tgcgggatgc
    14161 cgtatcccgg tggatgtcct tatcggagcg gaaggcctcg cataaagcat cgtcctttgc
    14221 aagatgtgcg agaatgcgca gctcaatttg cgaatagtct gcggcaatca gccgcttctt
    14281 cttgtcgccc gccacaaaac cgctcctgat acgtcggccc agttctgtgc gcgtcggaat
    14341 attctgaaga ttgggattgc tgcttgagag gcgccctgtc gcggcaactg cctgattgaa
    14401 agaggtatga atgcgccctg tttccggatt taccagcttc ggaagcacgt ccacataagt
    14461 gctcctcagt ttttccaatc ctctgtattc aaccaaactc gccggaagcg gatgctcctg
    14521 agcaagcgac tcaagcgttt ccagatccgt ggaatagccg cttttgattt ttctgcccgg
    14581 tttcagcctc agttgatcaa acaaaatata ctgcagctgt ttaggcgaat tgatgttgaa
    14641 actttccccg gcaagatcat aaatcgtgta ttcaagtttt ttgagtttta ccgccaattc
    14701 ctgctggagt gcggtaaatt gtgatgtgtc cagagcaatt cctgccattt ccatggacgc
    14761 cagaacgcta atgagaggaa gttctatgtt gtccagaagc ggcagcaaat cctcttgtat
    14821 cagatcgggc ctcaaccgcc tttcaagcct aagcgtcatg tccgcatctt cacaggcata
    14881 ctccgacgcc gggccggggg gaacctgagt gaagggaatg gaatgagcgc ctttaccgat
    14941 aagatccgtg atgggaatca tcttatggtt caagtagagc agactgagct catccagatt
    15001 gtgcctcaga cgggaggcat cagtcaaata agatgccagc atggtgtcca tggcaatgcc
    15061 ctgcaggtga ataccggcgc gggcaagaac gatcatatcg tacttgatgt tgtgtccgaa
    15121 ttttttatac tgcgcatctt ccagcagagg cttaagtgcg gcaagtgctt cttcggtact
    15181 gaattcaggt gcggaaaaat caactcccgc catgagcgca tcaagatcgg gtgcctcatg
    15241 tcccagagga atataccagg cacggcctgc ttccgcacac acggaaatac ccgccagacg
    15301 ggcaagcatg ggatggatgg aggtcgtttc cgtgtcgagc gcaaaggcac ctgctttttt
    15361 taaggcgtcc acggtctgct gcagttcgga acgggtcatt accagatgat actgatgctg
    15421 cacagctgcg gggacatttt tttctttact gaaaagttct tccagaatgc tgtggaaaga
    15481 aagggcgcga aaacatgcct ctacgtcctc ttgtttccat tctttgcgct tgaaagtttc
    15541 cagatcttcc tgaagtgcga catctgttct aagggtgacc agctttcttg caaaaaaagc
    15601 ctgctcttta tctgcttcaa gattctcacg ctgttttcct ttgaactcgt ccagatgagc
    15661 atagagattt tcaagcgtct gatattgaga gagaatcttt gccgctgttt tttcgccgat
    15721 acctcgaacg cccggcacgt tatccgctgt gtcgccgatc agtgcaagcg cctgcggtac
    15781 aaattcaggc gtaacgccga agcgcatttt gacgtcatcg gggccccacc aggctttact
    15841 ttcatccttg cccggatcaa acatacgcac ctgtttgccg atcagctgaa gcaggtcttt
    15901 atcgccgctg acaagaacga catccattcc ggctttttca gcctgaactg ccagcgttcc
    15961 gatcacatca tccgcctcga cgcctggcac cgtcaatgcc gggaggttca atgcctcgac
    16021 gacatcataa atccggggaa actgctcaat cagttcagga ggggtcgctt tacggtttgc
    16081 tttgtattcg ggaaacattt cttcccggaa attcttttcc cgtgaatcaa agacaacggc
    16141 aatataatca ggccgctgtt cgcgcagaat tcttaaaaga atccgggtga atccgtacac
    16201 cgcattggtc gggcggtgat tggcgtcggt gagattggca ttgatggcat gaaaggcacg
    16261 gaaaataaaa gccatagcat caatgagata aaaggtttgg ttcataaaaa atcagccgtg
    16321 taacgctgtc tgaaaacaga aatcagacaa caccgattgg caaaggcaac gtaccgccgc
    16381 accatgtccg caacagataa aacgcttggg acgcatgcgg agagctgctt gattaaaaag
    16441 gcgtgcagcc ggcacagaag actatttcag cagccggcgt ccctctgaag acttgatgcc
    16501 ggaaatgatg cgctggaatc cctcttcgtt ggcgcatgcg ctgatggctt cctccttgct
    16561 gaccagccct ttctgataga gttccacagc gtactggtca aaactgcgca tgtcgctatc
    16621 gttttcaatt ataccatgaa gcttgtcgag atcgccatct aaaatagcgt cacggacaat
    16681 gggcatcccg ccaaggagaa tttccacagc agggattcgc ccgccgccga ttttcttgag
    16741 cagacgctgg cagacaacac cctgcaggga ataggcgatt tcctgacgga tcaggtcgcg
    16801 ttcattttgc gggaaataac tgatcatacg gttaattgtg tctacagccg tcgtcgtgtg
    16861 cagcgtgctg aaaaccaggt gacccgtttc cgctgcgctc agaccggcgc ggattgtttc
    16921 catgtcgcgc atctcgccga ccagaatcac gtccgggtct tcacgcagag agccgcgcaa
    16981 agcgtttccg aaagagtgcg tgtcacggcc cacctgacgc tgggaaacaa tgctcttttt
    17041 gtcgacatat acgtactcga cgggatcttc aatggtgatg atgtgggcac gtcgctgctc
    17101 attgatatag tcaatcatgg cggcgagtgt ggtggattta ccgcttcccg taataccgca
    17161 gacgaggaaa agaccgcgat gtttatcgca cactttccgg actacatcgg ggatattcag
    17221 tgcatcaaag ggaagaggct gttccggtat gagccgcaat gctattgcca tggcaccgct
    17281 ttttatatag ccgctgcagc ggagatagcc gatgccagcg gcgtggtatt gaaaactgga
    17341 ttcacgttcg cgctcaagca tctgtaattc agcctcgccg cccagctgca gcacaaactt
    17401 ttccatgtca tctttggaaa tgaccggcgt atcttccaga gggagcaggt cgttatcgac
    17461 acggataaaa gggcggtttc cggcacgcaa gtgaaggtca gatgccttct tttctttcat
    17521 gatttcaatc aatctgttga tattgaaacc attgtccaca tattcggaga cttccagttc
    17581 ccaggtttcc gagcgcagac tctttgaaac aaattgttct atgatttgat caagaggaat
    17641 cagggcaggc agttggcgcc attcaaagga aggcacacta ataacgagtt gaccgttttc
    17701 ctgtcgcatg cggaagccga gccgggttgc gttcagcgca ttttccactt cggacggtgc
    17761 tacgccttca cggggtgtga agtaaatgcg catggtatca atgcgatttg acagattttt
    17821 cccctgcacg gaaatggtgc agttatgcgt tttaaattcc gcttcagtgg ggaggccggt
    17881 cgcccgaatg atttcggtca taccaatgcg gaacacgtcc aaaagggtgc tggttgtctg
    17941 gctgccgata agcataggca cattcctgaa aagttagaat aggcagagcg gggttatatt
    18001 ggggtatatg aagaggaggc cctgcctgaa gccattacct gaaaaggtga tccattggtc
    18061 aatttatacc atgattcccc gctaaaaagc aaaataaaat ataaaaaaag gagcggcgcg
    18121 ctcagcgatg cccgttgtcg tgcgtggctt ttcaatttga cggtgtccaa attttcaaaa
    18181 gagtggttgt ttttcttttt gcctggcaac agggtgcatt acttcgcctt ttgcggtaga
    18241 atagagactc agtagaatac aggaaggaac ggtctcatga gcgaaagagc ttttatcgaa
    18301 acatttttac gggcgaacag cgactacgtt acgcaaaaat accaggaaaa gcagaacgtg
    18361 accgtaacca gcaaggacag cccggttaac ctgctgaccg aagtggattt gacgatccaa
    18421 aagcgtttta tcgatgccac agcagaggtc tggcctcatg acttggtggt aggcgaggag
    18481 agtgggcatg accgttatcc tgccgatccc aatggccgca tctgggttat tgatcctatt
    18541 gatggcactt ataatttcgt ccgtggcctg aatccttctt ttgccatcag tattgctttt
    18601 atgcaggacg ggctggttca ggcggcagga gtgtccatgc ctcttgccgg ttattatttt
    18661 tttgcggaag cgggcggcgg ttctttctgc aacggtcggc gcctttctgt ttcccgggtg
    18721 cagcatctgg agagcgcctg tgtggaggtg gatttcagtt ccgttaaaaa caggcgaacc
    18781 atactccggc gcggcgcaaa gattatccgg gaagccgccc agttgagaag ccacggttcg
    18841 gcggtagcgg ctatttgcca gatagcgacg ggtgatgctg atgcctatgt tcaccccggg
    18901 ctgcacccct gggattatgc ggcggcgcag ttgattgcag aagaagccgg ggcactggca
    18961 acacggctgg acgggcagcc tctgcgtgtt tttgataaga agaaaggggt actggttaca
    19021 aacggagcgc tccatgagca gcttctggct tcgcttaccc gatagcatcg ttctttcaat
    19081 gattgaataa aaagggaaaa taatggatct tttacgggaa tattttaaag gtgataaact
    19141 ggcggcaacg ctgggcatga ctattgaacg cattgagccg ggcgcagcgg tagtaacgat
    19201 gcctcttgcc gagtgtcatc agaacgggct gggcagtgct catggcggag ccttgttttc
    19261 tctggcggat ttctgctttg ctgtcgcatc gaattcacat ggcagagtgg cggttgctat
    19321 taactgcacc atttcgtttc accggggaat ccgaagcggc gtattaacgg cgacagcccg
    19381 ggaaattcat tgcggggcga ccattgctgc atatattgtg gaaattacag acgagcaaca
    19441 taagtcgatt gcgacttttc aggggcaggt ctttcggaca cagaacacca tagaggcagt
    19501 tcttgccgaa aggaaggctg caggacagga aaaagatccg gactgatcag aacagaaatt
    19561 cgggctcaag taccggttcg aagttgacat ccttgtcgct gaaggggaag gtaagggttt
    19621 cgaacatgcc tgttccggta cgcatgacgg cacgggcgct gcccagcacc ggcgcaacac
    19681 ctaccagata acccagaggt ttgccctttt taagattgcg gtcaaaagtg accgggactt
    19741 cggcccagcc gaaaagaata ttggaaatgc cgcgacccat tttggtgagg gatttttcca
    19801 taccggtggg cttgggcaaa tccacatcag gattatataa ctgagcctgc gcagaggcgg
    19861 caaagagcag agccacagca aaactcagaa tgacagcaga accgattata tgcttacgca
    19921 tggtataaac cctccggtaa aaaaccgtat gttcagtaac ttccagtaat aagtctatca
    19981 aatttgaagc atattgtcaa caatttattt tccaaaagaa aaagaaaagc cagagcggcg
    20041 tatccttaca ggctggacgc attttccttg attttccgaa tgatgctgac aatgtcgtgc
    20101 atatcttctt ccgtaccgag caaaatctgg tggggaagcc acatgccact cttagcgcaa
    20161 agatcttccg tgaacggaca ggacacggtg ctgtagtcat agcaggtgag attctggaat
    20221 acgggctgtt tataaatcgg catgggatag cctgccgtga tgggaagccc ttccgccacg
    20281 gcagcttcta caaaacgctc ccgggtacag ccaaaagcgg actcatccag gcgcagacag
    20341 tagaggtgcc aggcgcggcg tgtgattctg ttgcttttcc gctggagatg gagcccttca
    20401 atttccgcaa gcccctgatt gagaatggaa gcgttttctg ctcggcgcaa tatatgttct
    20461 tctgcacgtt ccagctggca cacaagaata gcagcctgaa actcgctgag gcgggcattg
    20521 gtgccgacat tcaggtgata ataccaggga gagccgggct tgccacgccc gcagttggtc
    20581 agcgatcgga cttttgtcgc cagttcctca tcattggtca cgataccgcc gccctcaccg
    20641 gcagtaatat ttttggatgc ctgaaagctg aaaaaaccac aggctccgat cgttcccgtg
    20701 ccctgacttt tccagcgacc gccccagctg tgacatgcat cttcaatcac gggaatgccg
    20761 tggcggctgc caagttcatt cattttatcc atatcacaga tgcgaccgcc gaaatgcaca
    20821 ggcatgatgg cgcgggtgcg cggagtgatg gctttctctg ccagatccgg atccatgcac
    20881 cagctgtcgt cgagatccac aaagataggc gtggcaccca cgcgtagcac ggcgctcgcc
    20941 gttgcgatga atgtataagg gggaaccagc acttcatccc ctgcaccgat tcccattgcc
    21001 tgcaggataa tttcggcagc tgctgttccg ctgttgcagc tgatgcaatg atcagctccc
    21061 tgaaaagcgg cgtaagcggc ttcaaattcc tgaaccttcg gaccgaaaag ccaactgtcc
    21121 cgatggagca cctcaagaag tgcgttttct tcccgttggt cgtactgggg ccaggcaggc
    21181 cagtttttat cgggatggcg tacggctttg ccgccgagca gggcaagagt ggaagtggtc
    21241 atgaaagtct cccgttacgt ttaaaaaaaa gatgatgccc gatgtaccgc aaaaaggtac
    21301 tcatacttca tgcttattat gggataagcg aaatcatttt aacaagtctt tacccctctc
    21361 tccatattgc cggaagaaag aggctctttt tattgagggt tcccgtccaa aatcggtata
    21421 gttgctgcat cgggcactga taatttatac tgatttgtgc cctcttctgc ggctgacgct
    21481 ttcctcaata gaatacgggc ggggttcctg catgaacagt ccttacgata tcctccatca
    21541 ctctctatcg caaaaatcag cactggtcgc tattataggg ctgggatatg tcggcatgcc
    21601 gctggcaact tacttccacc gcagcggctt ccgggtgctg ggtgttgatc ttgacgagga
    21661 aaaacttgcc gccttgcgca gtggccgcag ttatcttaat catctgcctg ccgactggat
    21721 ccctgccgct cttgaagcgg agacgctgtt tcttacaagt gactacagcg cactgagaga
    21781 cgcagatgcc attttaattt gtgtgcctac acctttggga aaacacaggg agcctgatct
    21841 cagccacatc attcatgcga cgagccagat tgcctgtttt ttcaaaaggg gcacactggt
    21901 atctctcgaa agcacgactt atccgggcac gacaacagaa gttgttctgc ctctgctgag
    21961 taaaggcgga agagtggcgg gaaaagactt cttcctcgtt ttcagccctg agcgggaaga
    22021 tcccggcaac aagagtcatg ccctctccag tattcccaaa gtgatcggag gagtgacgcc
    22081 gcaatgcgct gcgctggcgc agttgctcta cggggcgatt ttcaccgaaa cagttcccgt
    22141 cagccagccc gccgcagcgg agctgtgcaa actgctggaa aatattttcc ggagtgtgaa
    22201 tatagcactg gtgaatgagt tgaaaatgct ttgcgggcgt atgggactcg atatctggga
    22261 gattattgat gccgccgcca caaagccttt tggtttcatg cccttttacc cgggacccgg
    22321 actgggcggg cattgcattc ccattgatcc tttttatctc acctggaagg cacgggaata
    22381 cggatttgcc acacgtttca ttgaactgtc cggcgaaatc aatacgtcca tgccccagta
    22441 tgtcctgtca cgctgcatgc aggcgttaaa catgacaggg cgccccctga gaggttcccg
    22501 ggtgcttgtt ctaggtgcag cgtacaaaaa agacatcaat gacttgcgtg agtcgcccag
    22561 tattccgctc atgaatctgc tggaggaaaa cggcgccatt gtgcagtata acgatccttt
    22621 tatcccgcat attgccgcca tgggcgctaa cagtcccgaa cggaacagca tagaactgac
    22681 aagtgcagcc ttaaaagaca gtgatctggt gctcatcgca acagcgcaca gctgttatga
    22741 ccccgcattt atcgttgagc atgcgccgct ggtggtggat acacgcaata tgaccggcaa
    22801 actgcttgag aaccggcata aaattgtgca ggcatagctg ccgcagcagc cgtcagcaca
    22861 tttcccagat agatgcctga ccgatgccgc cgcctacaca aagcgttgcc agacccagcg
    22921 aactgccgcg ccgcttcatt tcatgcagca gggagacaat gatgcgtgcg ccggtgcagc
    22981 cgaccggatg tcccagtccg atccccgaac cgttcacatt tgtaatgtcc cggttcaggc
    23041 aaagcagttt ctcacaggca agatattgag cggcgaaggc ttcgttcaat tcaatcagtt
    23101 caatatcatc cagagagagt cctgttctgc tgagcgcctg ctttgttgcg ggcaccggac
    23161 catagcccat gagctcgggc tccacgcctg ccataccata gcccttgagt cgggctatag
    23221 gagtaacgcc caggtctttt acccgttctt cagcaaccaa taccacagcc gctgcggcat
    23281 cattgatgcc ggaggcatta ccggcagtaa cggtgccgtc ctgtttgaaa ctcggtttca
    23341 gccgggccag ctgatccatg gtcatgccgg gacggaaatg ctcgtcttcc ttgaactgta
    23401 ccggttcccc tttgcgtgac ggaatggcaa caggaagaat ttcttcagca aaacgcccgg
    23461 attttgtcgc agcttcagcg ttgttgtgag agcgcagcgc aatgacatcc tgttcttccc
    23521 gggagatgtc atatttctcc gccagatttt cagcagtgag ccccatgatc agaccgcagt
    23581 aagggtcggt aagcccgtcc caaatgccgt cggacatcac gccatgctgc agacgcttgc
    23641 cccagcgcgc atccttcaag gtgtacggaa cccggctcat cacttcaaca ccgcctgcta
    23701 caaccacatc cagatctccc agcatgatct gctgggcacc gtagataatc gactgcatac
    23761 cggaagcgca attgcgatga atggtgaggc cgggcacttc aatcggcagt cccgccttca
    23821 gtgccgccaa ccgtccggaa gtggactcat cggcgcgctg catgcattgt cccacaatca
    23881 catcacccac cagttccgga ggcacatttg cccgggaaac tgcctctttg acgatcagac
    23941 ccatcaggtc ggaagcgggg atatctttta atgtaccgcc aaagctgccg atcgccgtcc
    24001 ggactgcgga tgcaattaca acatttctca ttgccgactc ctttttctta aaggctgcag
    24061 ttagtgtgtg aatagtgaaa catttagaaa acaaaataaa ttttagcact tcagtaagac
    24121 acatgccaac ttcttcgtgg gtaaaatgaa agagcggaaa gcatctacgg agaacaagag
    24181 ggcaggagtg tgaccccgga tacaggaaag tgtaaaaaaa ctgccggcgg gtatggggca
    24241 ccggccggca gcatgcaatc agggctgttc taaattcttt aattgcgtac gatcaaggct
    24301 tcccgctcag gaccgacgga aacatagcgc aggggacact caatcagctt ttcaatttct
    24361 tccacataat tgcgggcggc ttccgggaga tcctcatacc ggcgcacctg tgtcacatcc
    24421 tcatcccatc cgggcacttc catgtatacc ggcgtcgctt cttccagcac ggcattgagc
    24481 gggaaatcgg catattcttt tccctgccag gcatagcgcg tacagatctt cagcgttttc
    24541 ttgccgctga ggcagtcaag tttggtcaag gcgacttctg tcgcaccctg cattttcaca
    24601 ccgtatctgg tagcgacagc gtcaaaatga cctattgtac gggggcggcc tgtggaagcc
    24661 ccgaactcat gggcggaaat gcgcagcgcg tcgccttctt ccttatccat aagagcgacg
    24721 aaggggcctt cgcccacgca ggtggagaat gctttgacca cggcaagaac ggtatccagc
    24781 ggatagccca tcaatccgga gccgatgggc gcataggagg caagggtgca ggaagaagtg
    24841 gtgaagggat agatgccgaa gttgagatcc ctaagcgttc ccagctgcgc ttcaaagaga
    24901 atgtttttgc cttcttttgc ggccgcttcc agcagaaccg ttgtgtcggt cacatattca
    24961 cggagtctgt cgccatattt ttttgcccac tcaatcattt catcgacaga aaaaatgtct
    25021 ttttttccgt aaaaagcttc gagccgctcc agtttccagt tgctgatctg gcgcagtctt
    25081 ttttcgaaac gttcaggatg gagcagctcc cctatttgca atgccttttt aacggtgcgg
    25141 tcgccgtagg caggcgcaat cccgcggagt gtggagccat aggcgtcttt tcccagcctg
    25201 gcttcttcca gcccgtcttc aatgcggtgg aagggaaagc agatggtcgc ccggtcggaa
    25261 atccggatga cgggctggat gtttttggat gcgaggtcat ccaattcatc gcacagccct
    25321 tccagatcaa tgaccatgcc gggcccgaga atattggtga cgccgggata aaaaacacct
    25381 gacgggagca agtgcagttt gaactcgccc agatcattta caacggtgtg gcctgcatta
    25441 ttaccgccct gataacgcac cacaaaagcg gcgtcccggg ccaggtagtc caccatccgc
    25501 cctttgcctt cgtcgcccca gttggcaccg actactgcat caatactcat atttgttctt
    25561 tcttctctta tgaaaaggat taggtttgga ggtgcatcgt acgggatgcg ccattgcctt
    25621 acgttcaggc gcccttcatc atatcatgtt atgttccgaa tcaaaaaacc ctggattgcg
    25681 atcctgagcg caggtaaaac aaaatggcga accgcccgga gcgattcgcc atgatcataa
    25741 acaagcctgt gaattgtatt accgtgaata gaaaccgata acacttgtcg tatcaaactc
    25801 aaccgggaag ggtacattgg gagtcgcagt cattttgcct tcaaaggatt tggcatcacg
    25861 ctgcaggtat tcggggatct catagggcgg gttgttcacg ccatccacga ccatggggat
    25921 tttccggctt cgtgcacgga tggaaatgac catgccgggc ttcacctgga aagaagggat
    25981 attgactttt ttgccgtcta cctgtacatg gcaatgattg accaactggc gggcggcaaa
    26041 aatggtgttg gtgaaaccca ggcgccagac aacggtgtcc aaccgggatt ccagcaacat
    26101 catcagattg gtgccggtgt tgccgccgag gcgacgggcg ttatcgaaaa tacgccgcat
    26161 ctgccgttcc atgataccgc cgtaatacat gcgcatcttc tgtttttcca ggagctgcac
    26221 tccgtaaaca gagagcttgc gccgaagatt tttgccatgc tgtccgggcg ggtaggggcg
    26281 tttttcaaca actttgttgc tccgtcccca aatgcatttt ccaaccctgc ggcttaatct
    26341 gttttttgga ccgtgatatc tcgccattgt taagagaaac ccttttgcgg gagcgtatgt
    26401 cccgtctttt ttttcgatct gatccgcttt caggcaggaa acacctgatg aagcacacaa
    26461 cggattacaa aacggtatcc gcaggaatgg gatactgtga acagccctcc gagcaggcac
    26521 ccgtaagcgg tagatccgat agtgaaaaaa aaagaatggc gtacccgaga gggcttgaac
    26581 ccctaacctt ctggtccgta gccagacgct ctatccaatt gagctacggg tacgcagact
    26641 ttggatgcat tgggtatttt aacaaaagag caaaggaatt gcaagtttat accttaaaga
    26701 gcatcgggca gcccaaaaac tcagcgccat tggatcaatg cctgattgag acattcttcc
//
LOCUS       DAOPWB010000031        26227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1849_length_26227_cov_5.076609, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000031 DAOPWB010000000
VERSION     DAOPWB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 26227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26227
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1849_length_26227_cov_5.076609"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="secY"
                     /locus_tag="PKN92_04600"
     CDS             <1..1179
                     /gene="secY"
                     /locus_tag="PKN92_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNZ48104.1"
                     /translation="GRGSGLLGFYDLFTGGAFSQATVFALGIMPYITSSIILSLLVNV
                     IPALENLQKQGQEGQRKINDYTRYGTIFLCIVQSIGIAVYLQSMGPTIVPHSGVFFIA
                     LTMITLTAGTSFIMWLGEQITEHGIGNGMSLIIFTGIVSGMPRAISNMFNLLRTDQIS
                     IAVVLVLAFMMVFVVAGAIIVTTGQRRIPVQYPKVVNQRRVSGGQRTYLPLRVNQAGV
                     IPIIFASSILMLPDALVRSLHVPGWLETPLAIALNPQGVIYNAVYALLIIFFSFFYTA
                     STFRPAEMADNMKKHGGVIIGVRPGKATADYLGRIMSLVTWSGSVFLAIVALVPVLII
                     QILQVRDMTIAHYFGGTTLLILVGVALDTAKQIEQHLAMRHYEGFGSAKGGRIRSRRM
                     "
     gene            1304..2080
                     /gene="map"
                     /locus_tag="PKN92_04605"
     CDS             1304..2080
                     /gene="map"
                     /locus_tag="PKN92_04605"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNZ48105.1"
                     /translation="MIAIRTEKEIDLMRQANAIVAHAHKLAATMIQPGVRTDEIDRAV
                     EALILSEGGKPAFKGYRGYPASTCISIEEEVVHGIPGARKLQAGQVVSIDIGASYKGY
                     FGDAAVTHACSGVDLLRVRLLDVTDLALSRAIRQARTGGHLNDIGKVIEQTAHDAGFS
                     VVRDYVGHGIGTQMHEEPQVLNFDNRMPGPELREGMVLAIEPMLNAGTDEVRSKSDGW
                     TVVTADGKPSAHFEHTVVVREAGGEILSWCDAPMWGDKQD"
     gene            2131..2346
                     /gene="infA"
                     /locus_tag="PKN92_04610"
     CDS             2131..2346
                     /gene="infA"
                     /locus_tag="PKN92_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010619611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNZ48106.1"
                     /translation="MKEEAIEVEGKVMETLPNAMFRVELKNGHRILAHISGKMRMHYI
                     RILPGDVVKVEMSPYDLTRGRITYRQK"
     gene            2458..2826
                     /gene="rpsM"
                     /locus_tag="PKN92_04615"
     CDS             2458..2826
                     /gene="rpsM"
                     /locus_tag="PKN92_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNZ48107.1"
                     /translation="MARILGVDLPREKRVEAGIQKIYGIGPTRAKELLKKASINPDTR
                     VRDLTDEEVNVISTLIQTDYKVEGDLRREITANIKRLMDINCYRGTRHKRGLPVRGQR
                     TNTNARTRKGPRRLAVVKKK"
     gene            2892..3290
                     /gene="rpsK"
                     /locus_tag="PKN92_04620"
     CDS             2892..3290
                     /gene="rpsK"
                     /locus_tag="PKN92_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNZ48108.1"
                     /translation="MAKEKRKVKAKKRKVHLTVTSGVVHIQATFNNTIISITDAQGNV
                     ISWSSAGQVGFSGSRKSTAFAAQLAAEQAGKKALELGLKEVRAYVNGPGAGRESSIRA
                     IQACGLTVTQIRDVTSIPHNGCRPPKRRRV"
     gene            3398..4459
                     /locus_tag="PKN92_04625"
     CDS             3398..4459
                     /locus_tag="PKN92_04625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNZ48109.1"
                     /translation="MIEKQLTIPRNYHVEPTEDGLGVRVTAEPFERGFGTTVGNSLRR
                     VLLSSLEGSAVTAIRFEDVPHEFSAIPGVYEDTTDIVMNLKRCQIRLNAENSLIFSFE
                     HSGEGTVTAEMLFKDQDVDVFNPDHVIFTATQKDTKVKMEVKVGKGRGFKTSDTFEFE
                     HAPLGTIYLDASFSPVVKVNYEIQDSRVGQMTDYDRLHMDIWTNGSISPEEALRQSAE
                     ILIEQFNIFAKDPQELEQEEGVDFGANAELVKLLSRSVDEFDFSGRVPNCFKAQNITQ
                     LGHLIVLEEDTVLRFPNFGKKSYEEVKQVLDSLKLEIGMPLDESVRALFTQREPVSVA
                     DSIDDVLDDDEEVDNQEED"
     gene            4465..4839
                     /gene="rplQ"
                     /locus_tag="PKN92_04630"
     CDS             4465..4839
                     /gene="rplQ"
                     /locus_tag="PKN92_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNZ48110.1"
                     /translation="MRHRKAGRRLGRDAAHRKATMKHLAVALFQHNAIETGVFKAKEL
                     RVFAEPLITLAKKDTIANRRHAFAKLRDESIVKKLFTEIGPHYAERPGGYTRILRLGN
                     RVGDGAEMARIELVELGEYKAD"
     gene            5013..7079
                     /locus_tag="PKN92_04635"
     CDS             5013..7079
                     /locus_tag="PKN92_04635"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HNZ48111.1"
                     /translation="MKLRQVTPVLCSMSRSVISSASCMLLFLLLAGMPVTFCATAEPA
                     VKNWHEDAFFGLHYDLHPGAGDTELGRLCSYEHIRAMLEKVQPDFVQYDCKGHPGYTG
                     YPTKVGSPSPGIVNDALRIWRDVTRDMGIPLSIHYSGVWDTRAIELHPDWARIREDGT
                     PDPNNTSRLSAYETELLIPQLLEVVRDYDIDGMWIDGENWASHPDWSAACQAAFTKET
                     GITDIPKKSGDPHWLEWLAFQRALFVKHVTDYTNAVHQLKPECMVTSNWMYSVRQPEE
                     MEAPIDYLSGDFDPSFGSERAMAEGRFISSRGKPWDLMAWSFLQIHDQGWTTKPAVHL
                     CQEVSTVLAQGGAIFIYDQPQRSGKLTDWHQDIFAEVAQFCRKRQALSHRTESIPQVV
                     VLHSENSYYRYNSPLYNFGGANAAMEGALFALLENGYSVDIMNEATLGARLSEYPLVV
                     IPEAENVSDAFKSKLSAYVEAGGRLIVSGAHVALQYGDLAGVSPASGTAIGWLPAGNG
                     AVKCSGSYQKTVLNGARVLHPVLENQSIPADDKELVPASTLNQVGQGSVAAIHGPIFR
                     NYFQGRYPGLRQVIGEVAAALETPGLSRTCAPWYVEMALRKKDGRTLAHFVNRSVSGY
                     QSSYYHLVEHVPDAGTFSIEIPMAEKPQRCYMSPDPVGLEWHWKDGVLSANISALHIH
                     NILVIE"
     gene            complement(7069..8955)
                     /gene="nadE"
                     /locus_tag="PKN92_04640"
     CDS             complement(7069..8955)
                     /gene="nadE"
                     /locus_tag="PKN92_04640"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNZ48112.1"
                     /translation="MKLIKTGLVSLSVRTGDFSGNTALLMDAIEEAGKAGVQLLIAPE
                     LAVSGYNLQSLGMWRDTARQSWASLAEIAAACDGMTAIVGVPVLYHGSLRSGAAVISD
                     KIISGIVLRETAAAFPSLSRIDSLLNGVSAGQLSFDMPWGTLLPSFDTDTLVSPPSFL
                     NGDGCATVICCMAAVPFASGYAERRRIRMLDTARRLHGLCVHTNLLGTDDGRLIFDGT
                     GLIAAPSGILEETRAFSAKQWTLNTVIVDLGELERTPPLLPVSLELEGAEGCCIGISC
                     AQAAFKPASVRAYAKQIAAQAERAGRKSETNNSNQDLDAILHAIALGIRDYYRKAGVF
                     RKILLALSGGQDSALCLLAAVQAARMLAKDSGTDDYASFIQTLYLPNRALSSTAGHDA
                     ARALAEELHIPFRVVAIHEEVDIAFSKAVEIMESKKKVRPITRQNLQARIRGDIMLNW
                     ANNVDGLVLVTTNMSEVAVGYSTAGGDNQGAYSPLANMPKTLVSRLLAYLEERDGLQS
                     LKKVLALKPSAELAPDQTDEDELMPYPVLDEIIHLHIGKRLSLSLCWRMLAEKFPDHG
                     PETLREWIARFGKRFAASQWKRNQHPVAPKVLDYDLDAKSGIALPVLHRMQSELDDLK
                     NATL"
     gene            complement(9009..10469)
                     /locus_tag="PKN92_04645"
     CDS             complement(9009..10469)
                     /locus_tag="PKN92_04645"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNZ48113.1"
                     /translation="MNTLHNPWFQREGLALLTDFYQLTMMAGHWKEERVDRELSFEYF
                     FRYLPPNTGYAIAAGVEEFLHYFEHLYFTESDLEYLRSLKIFDDCFLEYLRHFEKKCT
                     IRCVPEGTAVFPFEPVIQVEGPILEAQLLETALLNIVNYQTLIATKASRICLAADGDP
                     VMEFGLRRAHGPDGGLSASRAAFIGGCHSTSNVLAGQLYGIPVAGTHAHSWVMSFSSE
                     LEAFRTFARLYPERCILLVDTYDPVKSGIPNAIRTFCELREQGVPIRPAVRLDSGDLA
                     KLSKICYSMMLQAGLENPLIVASNDLNEDLIADLKRQGARINAWGVGTQLVTAYDAPA
                     LPGVYKAVAMKDTSGKWSPVIKTSFNYEKATDPGRKYPQRYLNDEGYILGDVMYSEYE
                     TPETGGIIEGRSRKHPQVPRRIENAAQSQPLLREAFVLGKRISAERTAAEIREYAAAQ
                     QSGLTEEFKRLRNPERYPILLSPAMGDLKHDLLLMH"
     gene            complement(10466..11038)
                     /locus_tag="PKN92_04650"
     CDS             complement(10466..11038)
                     /locus_tag="PKN92_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012570973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNZ48114.1"
                     /translation="MVNVNDTDALIIVDVQNDFCSGGSLPVPDAELIIDPINRLQRFF
                     DYLYFSRDWHPNDHCSFRDNPHFDEGCWPSHCVQNSPGAEFHGDMRIPVDATIIDKAT
                     TAEKDAYSAFDGTDLSTRLERRKTKRVFVVGLALDFCVKATALDALQNGFKAFVIRNA
                     TRAVFPENEAAVYAELQAAGVELIDSEDLS"
     gene            complement(11043..11486)
                     /locus_tag="PKN92_04655"
     CDS             complement(11043..11486)
                     /locus_tag="PKN92_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNZ48115.1"
                     /translation="MTLFSYDYPRPMVTVDAVVFADGSGEKHVALVRRKNEPYKGSWA
                     LPGGFVEMDESLDKAVMRELQEETGLRNVPLTQLHCFGDPGRDPRGRTISVVFAGELL
                     SKEALCANDDAAAAEWFPVNALPPLAFDHAAILAFVLAHFNFSQD"
     gene            complement(11627..15235)
                     /locus_tag="PKN92_04660"
     CDS             complement(11627..15235)
                     /locus_tag="PKN92_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ48116.1"
                     /translation="MEESSVRLSIERRFILSILWVGVIPMIMVLLIGYFAAREGQQLA
                     VMQNLLTAARKTVGGVSLVIQERERNTTRIAQYPEIVDWLRHAYRSETGSDQALRRRL
                     LQEVHESPTLHSSFYLYNSAGKAIFSLDDTEPPPLPGLDKITETRFLRPDYLNSQGRY
                     TALLVAPVFDANKEIVLGYLVENQSAQELMELILDDNFVVEGRRRDRYEIIYMAEDTQ
                     FTVSLNDDSETSPPPPRVTAVHPKLAERLKNTEKTQDAFVLWDYLSQGVRTPVLIAYQ
                     RLASYHPLYVAVHQPTPAVFRTINMAAGVALLASCLIIALFCAIAYRIVNNKILRPLS
                     LLNEGAQIIRQGDLDLKLKIQTGDEIEELAASFNQMAAALRSNMHRLGASQEKYRHLI
                     TSLRDCIFQTNQEMQIIFINPAGVAILGYSWRDEVLGRPLGDFFVDEDDYQKFIQNIK
                     RRPYVDSMRVWLSKFSDEPVCVEISGNRILSDQGNMQGIEGSFRDVTRSLRMEKEIAE
                     RAERMATVNKIANVVNSSVEAGRVYESLLAELHRLVDYDYAAVSITLDANAHETRQIW
                     PEPQDGRERFPRLDDDHSCAGWVAKAGRPLNIENIKKENEIMACQFPPEICSVLCVPL
                     RAEQQILGSLQLGSNDIGAFSDHDLHLFESIAPHLAGAIRNAQLLENLKITLDQVNSA
                     KEKLDSANKELKSLDEMKTHLLSNVSHELRTPLVAVMGYADMILNEKAGIINDTQKEY
                     LEIILRNVEKLVTLIENLLDFAKIHRSTEGLVFTSFDLIDCIRASMQVVKPVAEVRAI
                     SLHLTVHDEQGNTARPPVMVEGDKGNLGQVFNNLLSNAVKFNKNEGSVTVDVEVRKET
                     VLIAVIDTGIGIPEDGQDKIFTRFYQYDASSTRKYGGTGIGLSIAQDIVRLHGSRITV
                     SSTPGEGTTFRFSLPLHHPQSGGESTGTKPLPIETHLLIELVSQDRSLCAQIRQQLIS
                     EGMDVIHAAYPSAAVTLAQKHSPDCIIIDSETGPLASVLLEEFFSEAQDLKIPTLIIT
                     DNDSLYADYQELFDARLKRQFRKSTLLSGIHKALSPPVQTKSHLGNKILCVDADAETA
                     TFVQRCLSDEGFQIDIAASGDGALQLLEKGDYWLVLLDIALPDQDGWDLCKYIKSHPV
                     LSGIKVQIVTARSISQSMPQMRDSAADGYLLKPFKAEDILSLVRTFEIQRAQKPFA"
     gene            complement(15319..15900)
                     /locus_tag="PKN92_04665"
     CDS             complement(15319..15900)
                     /locus_tag="PKN92_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ48117.1"
                     /translation="MDREHKAYTTFEAAKICHVTHHSIKNWIKQGLIKASRTPGGHYR
                     ILEPDLDQFREQYDMFPRDKAFTSRHIMIVDDDQDALALMERILVDEGYNLVMVSNAT
                     EVGLKAAQTIPDLILLDFLMPEINGFDVCRALRNNEATRNIPIMAVTCLDKEQDIERV
                     FACGADDYLAKPFKVDHLLSKVRELISRKGDQD"
     gene            complement(16432..17364)
                     /gene="cysK"
                     /locus_tag="PKN92_04670"
     CDS             complement(16432..17364)
                     /gene="cysK"
                     /locus_tag="PKN92_04670"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNZ48118.1"
                     /translation="MMAFYQDNSLSIGKTPLVRLNKVTADSKAAHVLAKVEGRNPASS
                     VKCRIGAQMVWDAEKRGLLKPGGTIVEPTSGNTGIALAFVAAARGYRLVLTMPETMSL
                     ERRAMLKAFGAELVLTPGEKGMPGAISTAEQIAEENPDFFLPQQFKNPSNPEAHFRTT
                     GPEIWEDTDGAIDVLVAGVGTGGTITGIGRYIKKEKKKQIECVAVEPVTSAVLSGEKP
                     GKHKIQGIGAGFIPDVLDLSLIDKIETISDEKAFEMARRLAKEEGIICGISSGAAAAA
                     ALRLARNDEYEGKTIVVILPDTGERYLSTPLFQY"
     gene            17625..18638
                     /gene="argC"
                     /locus_tag="PKN92_04675"
     CDS             17625..18638
                     /gene="argC"
                     /locus_tag="PKN92_04675"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNZ48119.1"
                     /translation="MIKVGIVGATGYGGRELLRLLCAHPQVTVTAAASTSVSGDALAD
                     ELPAFAKLIDLEFETFDATSLAARCDVVFVGVPGKASMAPVAALHEAGICVIDIGPDF
                     RLQDLALFQKYYGVEHIAPELVQKAVYGFPAWYRDALRQAEIIAVPGCYPVSMLTPLR
                     PLTDAPVSGVPIVIDSISGISGAGKSLSQAFHFPEMNENLKAYKIGVHQHIPEVEQEL
                     LNRFTIQFTPHVGPLTRGILTTITLRPETLFDPAPYYACYADEPFIRVLGAGKTPDIN
                     QVRASNFCDFGWVCDERTGNIVMVSAIDNLMGGTAGMAVQCMNIRFGLEENTGLKWAG
                     MAP"
     gene            18635..19843
                     /gene="argJ"
                     /locus_tag="PKN92_04680"
     CDS             18635..19843
                     /gene="argJ"
                     /locus_tag="PKN92_04680"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNZ48120.1"
                     /translation="MKKISGGLCAPAGFRAGAAAAGVKKANTTRLDCALIVSDRPASV
                     AGVFTTNRVFAAPVRYCREICARGRARAFLVNSGNANACTGESGYKDVLSIAACAGAL
                     LDIPADEICVSSTGVIGVPLPLDRLSGGINQCAASLSSEGSAAAARAIMTTDTRPKEM
                     ALEIALSGGTVRLGGIAKGAGMIAPNLATMLAFLTTDAALDPPLLRRLLKEAADVSFN
                     CICVDNDMSTNDTLLIMANGASGTPLLKEGTADSRLFAEALQRLCADLARELVLDGEG
                     ATKFVTIAVEGAKDTASARQMAATIAKSQLCKTAFYGSDANWGRIVCAAGYSGVVFDP
                     SRTDLYIQGVHVFSNGRPLPEKDSEVQPLMASPEITVRLCLQEGTASGEYWTSDLSID
                     YVKINADYRT"
     gene            19859..20752
                     /gene="argB"
                     /locus_tag="PKN92_04685"
     CDS             19859..20752
                     /gene="argB"
                     /locus_tag="PKN92_04685"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNZ48121.1"
                     /translation="MQELIKKAEVLIEALPYIRQFFGKTVLIKYGGAAMLDPALRAST
                     IQDVVLMRYVGMNPIIVHGGGPAINGMLKRLDIQSHFTAQGLRVTDSATMEVVEMMLA
                     GSVNKDIVNLINCAGGEAVGLCGKDGRMLYARKKLAESGEDLGQVGEITSVNTRVLTA
                     LCESGMIPVVAPIATDEDGGTWNINADTAAGDIAAAVKAEKLVFLTDTPGLLREVKNP
                     ETLIKQVHISEVQRLKEQGIIAGGMVPKVEACIKALNHGVRRTHIVDGRVPHSMLLEI
                     FTDKGMGSLVTRDTAVVQEGN"
     gene            20759..21400
                     /locus_tag="PKN92_04690"
     CDS             20759..21400
                     /locus_tag="PKN92_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNZ48122.1"
                     /translation="MDTESYFVNIIIQGGILMWPIMMCSVIGLAIVIERFFVLRRADI
                     DTREFMDVMRQVLRQNRIREAIEICDETEAPVARILKAGILKHDRSKDDIREAIEEAG
                     RFEIPRLERHLSALATCSTIAPLLGLLGTVQGMIKAFSQIQNKRGQVNPSDLAEGIGN
                     ALITTAAGLTVAIPILVVHNYFITRVDNMVLEMELSSSELIELLTQHHGDREI"
     gene            21403..21843
                     /locus_tag="PKN92_04695"
     CDS             21403..21843
                     /locus_tag="PKN92_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNZ48123.1"
                     /translation="MKFKRDNRRKVLASLDMTPLIDVVFQLLIFFMVSSTFVVQSSVP
                     IELPEAEGADQLESKEMSITLVYGAGGPEDGGEIFVNDISITNWTQLTRSLVELRERD
                     PEALVLIRPDARVPTERLVKVLGFANSVGIKNYGIAATSPTSER"
     gene            21873..22742
                     /locus_tag="PKN92_04700"
     CDS             21873..22742
                     /locus_tag="PKN92_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48124.1"
                     /translation="MTAEKAVYPDPIAYRRRLQRKNRKRLFWAAGFSLLFHLSMITFF
                     EIGVRYPRDDIRFYQVQIVSVPEVPRAAAPAAASSDALSLSGRNDYGQLPLIALPVVE
                     FAEMERLRVRYDTAAPLAELAERPTQLVYTDSWARFSSELQRMSRSLRDLALSEEGAV
                     PREKQPKEAQRLYFRPAEGFEAYIEWTGPPADRALLFSPPMKALWNVSTALKSRQLEI
                     VFQVDPGGRVVNVWSPEVEDAAVVDDIQMTLLQYRFSPLPELGMSGSGEGENRSLKDQ
                     SGVLFIKASEQAP"
     gene            22739..23116
                     /locus_tag="PKN92_04705"
     CDS             22739..23116
                     /locus_tag="PKN92_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48125.1"
                     /translation="MIQITLGTALLVYGTVIIVGFALLSLLGELRATGVYTVLEKQFM
                     WRCVFCSFLYLDQEAVTISRCPRCGSYNKSKEAQDERVSANREHWAPEPVSGPADPED
                     LPRRNPSRRKRPGQSRRGPRRRR"
     gene            complement(23231..25999)
                     /gene="ppdK"
                     /locus_tag="PKN92_04710"
     CDS             complement(23231..25999)
                     /gene="ppdK"
                     /locus_tag="PKN92_04710"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNZ48126.1"
                     /translation="MAKKSVYFFGGGKAEGTGEMKNLLGGKGANLCEMASMGIPVPAG
                     FTITTEVCTEYYENKGKLPQNVYDETEKALAKVEKVMKKKFDDPENPLLLAVRSGARM
                     SMPGMMDTVLNIGLSKNTLPGLIKKSGDPRFVYDSYRRLIMMYADVVMEKAAGIEPAD
                     GAGVRHLMEEAMDAMKEAKGVTEDTDLDAEDLKNLCEEYKVIVKKCFGKNFPDNPKDQ
                     LWGGIKAVFQSWNGKRAIAYRRIEGIPDNWGTAVNVQAMVFGNLGDSSATGVAFTRNP
                     ATGENKFFGEWLVNAQGEDVVAGTRTPSPLNDATKTDANRHMKSLQKEWPALYKQLAD
                     IRDLLEEHYTDMQDVEFTIEDGTLYMLQTRTGKRTGGAAVRMAVEMCESGLITEDTAV
                     MRVKPEQLDELLHPMLDPQAEKKEKYIAKALPAGPGGGVGRVVFTSEDAKEWAAKGEK
                     VILVRNETSPEDVEGMRSSVAILTSMGGMTSHAALVARGWGKCCIVGCAAVTINAREK
                     KFTVGNITVKEGDWISMNGTAGTVYLGQIPVLPAQPEKNKWYSKLMKWADARRQIGVR
                     ANAESQEDAAAALRFGAEGIGLARTEHMFFDADRIIYVREMILADNEADRRKAVMKLL
                     DYQTQDFVEIFTAMKGKPVTVRLLDPPLHEFVGSLLEPGAKKELEALANHAGLKPAQV
                     VERINALHEFNPMLGHRGCRLGIAYPEITEMQARAIITAAARLTKKKIHVLPEIMIPL
                     IGTREELANQRAIVEAVAADVQKETKTKVNYLIGTMIEVPRATLIADKIAESADFFSF
                     GTNDLTQMTFGYSRDDIGTFLPIYLEKRILDDDPFAALDQEGVGQLVDMAVQKGRSTR
                     PDLKCGICGEHGGEPSSVKFCHRVGLNYVSCSPYRLPIARLAAAQAAIETPRACSKPN
                     AKKCCKKK"
BASE COUNT         6170 a         6591 c         6982 g         6484 t
ORIGIN      
        1 gggcgcggtt ccggtctgct tggtttttac gacctgttca caggcggcgc cttttcacag
       61 gcaaccgttt ttgcactcgg tatcatgccg tatattacgt cgtccattat cctttctctg
      121 cttgtgaatg tgattcctgc cctggaaaat cttcagaaac aggggcagga aggtcaacgt
      181 aaaatcaacg actacactcg atacggcacc atttttctct gcatcgtgca gtctatcgga
      241 atcgctgttt acctgcagag catgggaccc accatcgtgc cgcattccgg cgtgttcttt
      301 attgcactta ccatgattac attgaccgcg ggcacatcat tcattatgtg gctcggtgag
      361 cagattacag agcacggtat cggcaacggc atgtctctga ttatttttac cggtatcgtg
      421 tccggtatgc cccgtgccat ctccaatatg ttcaatttgc tgcgcaccga tcagatctcc
      481 attgccgtcg tgcttgtgct tgcgtttatg atggtttttg ttgtcgccgg tgccattata
      541 gtcacgaccg gtcagagacg tattccggtt cagtacccca aagttgtcaa tcagcgccgt
      601 gtatcaggcg gacagcggac ctatctcccg ctgcgtgtga atcaggcagg tgtgataccg
      661 attattttcg ccagctctat tctcatgctt cccgatgcgc tggtgcgcag tctccatgtt
      721 cccggctggc ttgagacgcc gcttgccatt gccctgaatc cgcaaggtgt catttataat
      781 gccgtttatg cattgctgat tatcttcttt tcctttttct atacagccag cacattccgt
      841 cctgccgaaa tggcggacaa catgaagaag catggcggcg tgattatcgg agtgcgtcca
      901 ggcaaggcaa cggctgatta tctggggcgc attatgtctc tggttacctg gtccggttct
      961 gtatttctcg caattgttgc gcttgtgcct gtattaatca tccagatcct tcaagtgcgc
     1021 gatatgacga tagcccacta tttcggaggc acaacattgc tcattctggt gggtgttgcg
     1081 ctggacactg ccaagcagat tgagcagcat ctggcgatgc gccattatga aggctttggc
     1141 agtgcgaaag gcggaaggat tcgctcaaga cgcatgtaat gagtgccttc gaccgaatag
     1201 atgcgaagca gctcataaat atgctatact ataatgtttt aatccaacga atgactcccg
     1261 gaactagaat gcttggtctt aaaaattcac ggcggtgctt agtgtgatcg ctatccgcac
     1321 agaaaaagaa atcgatctga tgcggcaggc aaatgccatt gtagcgcatg cacacaaatt
     1381 ggctgccacc atgattcaac cgggtgttag aacagatgaa attgaccgag ccgtagaggc
     1441 gctaatcctg agcgagggcg gcaagcccgc ttttaaaggg tatcgtggct atccggcatc
     1501 gacctgcatt tccatcgaag aagaagtcgt tcacggtatc cctggggcgc gaaaacttca
     1561 agcgggtcag gtcgtaagca tagacatcgg agcttcctat aaaggctatt tcggagatgc
     1621 cgctgtaacc catgcgtgca gcggtgtaga cctcctccgt gttcgtcttc tggatgtaac
     1681 ggatctggca ctctcccgtg ctatccgtca agcccggaca ggcggacatt tgaatgatat
     1741 tggaaaagtg attgaacaga ctgcccacga cgcgggattt tctgttgtgc gggattatgt
     1801 gggtcacggc atcggaacac agatgcatga agagccgcag gtccttaatt ttgataatcg
     1861 catgcccggt cccgaattgc gggaaggtat ggttctggct atcgagccga tgttgaacgc
     1921 aggcactgat gaagtgcgct ccaaaagtga tggctggact gtcgtcaccg ctgatggcaa
     1981 gcccagtgca catttcgagc atacagtagt agtgcgcgaa gcgggcggag aaatcttgtc
     2041 ctggtgtgat gcccctatgt ggggtgacaa gcaggattaa gcgagaatcg gttgtccgga
     2101 agaaatacaa aaggataaga agcgaaaaga atgaaagaag aggccataga agttgaaggc
     2161 aaggtaatgg aaacactgcc taacgccatg tttcgagtgg aacttaaaaa tggccaccgg
     2221 atccttgccc acatttcggg aaaaatgcgt atgcattaca ttcgcatcct cccgggagat
     2281 gtggttaaag ttgagatgtc cccctatgat ttaactcggg gccgcattac ctacagacaa
     2341 aaataagcgg attcgagcag tcctttttgt cggacgggag cgattcgaga gagctaaata
     2401 attcgccgat cagaagaaag acggcataaa acaaaatgct ataggagtag aaggtacatg
     2461 gcacgtatac tcggtgtgga tttgccgcgc gaaaaacgag tggaagcggg aattcaaaaa
     2521 atttacggta tcggtcccac acgggccaaa gaactgctca aaaaagcgag catcaatcct
     2581 gacacgcgtg tccgtgacct taccgatgaa gaagtcaacg tcatttccac cttgattcag
     2641 acggattata aagtggaagg tgacctgcgg cgtgagatta ccgccaatat taaacgcctg
     2701 atggatatca actgctatcg tggaacccgg cataaacggg ggctccccgt gcgcgggcag
     2761 cggaccaata ccaatgcgcg tacgcgtaaa ggtccccgtc gtctggcagt ggttaagaaa
     2821 aaatagtacc cgtatcctca tctacggaat aacagtaaac agcatagcga actgctagga
     2881 gacagctgaa cgtggccaaa gaaaaaagaa aagttaaggc taaaaaacgt aaagtccatc
     2941 tgacagttac ctcgggtgtg gttcacattc aggcaacctt caacaatacc attatttcca
     3001 ttaccgatgc gcaggggaac gtgatttcct ggtccagtgc aggacaggtt ggtttttccg
     3061 gatcccgcaa aagcaccgcc tttgctgctc agctggcagc ggagcaggct ggaaaaaaag
     3121 cgctcgaatt gggacttaaa gaagtcagag cttatgtgaa cggaccggga gccgggcgcg
     3181 agtcttccat tcgggctatt caggcatgtg gactgaccgt tacccagatt cgtgatgtga
     3241 ccagcattcc gcataatggc tgccgtccgc ccaaaaggcg gcgcgtctga ctcggagttc
     3301 cgatgcagat ctactttcag ggccttttgc cccatcgctc tgcatccttt tcgttcgggt
     3361 tttctaacgt ggcctcagac tggagaagcg tgcaatcatg atcgaaaaac aacttactat
     3421 ccccagaaat tatcatgtgg aaccgacaga agacgggctg ggcgttcgcg tcactgccga
     3481 gccttttgaa cgtggtttcg gtaccactgt tggcaactct ctgcgcagag tgctgttgtc
     3541 ttctctggaa ggttctgccg taaccgctat acgttttgaa gatgtgcccc atgaattctc
     3601 tgctatcccc ggggtttatg aagatactac cgatatcgtc atgaatttga agcggtgcca
     3661 gatccgtttg aatgccgaaa attcgctgat attttctttt gaacattccg gagaaggtac
     3721 tgttacagcg gaaatgcttt tcaaagatca ggatgtggat gttttcaacc ctgaccatgt
     3781 catctttaca gcgacccaaa aagacaccaa agtcaaaatg gaagtaaagg tcggcaaagg
     3841 aagaggcttt aaaacatctg acaccttcga gtttgagcat gccccgctcg gcaccattta
     3901 tttggatgcc agtttttcgc ccgtagtaaa agtaaattat gagattcagg attcccgtgt
     3961 aggtcagatg acagactatg accgcctcca tatggatatc tggaccaatg gctccatttc
     4021 tcctgaagaa gcattgcggc agagcgctga aattctcatt gaacagttta atatttttgc
     4081 caaagatccg caggaactgg agcaggaaga aggcgttgat ttcggtgcta atgctgaact
     4141 cgtcaaactc ctctcccgct ctgtagatga attcgatttc agcggaaggg ttcccaactg
     4201 ctttaaagcg cagaatatta cgcagctcgg gcacctgatc gtgttggaag aagacacagt
     4261 actgcgtttc ccgaattttg gcaaaaagtc ctatgaagag gtaaagcagg tacttgactc
     4321 gctgaagctg gaaatcggaa tgccccttga cgaatcggtc agagcacttt tcacccagcg
     4381 tgaaccagtg tcagttgcgg atagcattga tgatgtcctt gacgatgatg aagaagttga
     4441 taaccaggaa gaggattaat aatcatgaga catagaaaag caggaagacg actgggacgc
     4501 gatgccgccc atcgtaaagc gaccatgaaa catctcgcgg ttgctctttt tcagcataat
     4561 gccattgaaa ctggtgtttt caaagcaaaa gaactgcggg tttttgctga gcctctgatc
     4621 acacttgcga aaaaggacac cattgccaac agacgccatg cctttgcaaa gctgcgggat
     4681 gagagtatag ttaaaaaact ttttactgaa atcggtccgc attacgccga acgtcccggc
     4741 ggctataccc ggattcttcg cctcggcaac cgggtgggcg acggcgctga aatggcgcgt
     4801 attgaactgg tggagctcgg cgaatacaag gcagactgat cctgcctgaa tttctacttt
     4861 acagcggcat agtttctatg ccgctttttt tatgcctgac ggcaccaagt tttatcttgc
     4921 agacgttttt acagataaca attatgctat gataaaaccg ctcatcaaaa aatgcttatt
     4981 tcaaaacaga agaagaaagg tgcatgcaga ttatgaagct tcgtcaagta acgcctgtgc
     5041 tctgcagtat gtcccggtct gtcatcagca gtgcctcttg tatgttgctg tttcttttgc
     5101 tggcgggcat gccggtaaca ttttgcgcta cagcggagcc tgcggtaaaa aactggcatg
     5161 aagacgcttt tttcggtctt cactatgatc ttcatcctgg tgccggagat acggaactcg
     5221 gtcgcctttg ttcttatgag catattcgtg ccatgctgga aaaagtgcag cctgactttg
     5281 tgcagtatga ctgtaagggg catcccgggt ataccggcta cccgaccaaa gtcggctcgc
     5341 cttctcccgg cattgtgaat gatgcgcttc gtatctggcg tgatgtgacc cgggatatgg
     5401 gcattccgct atccatccac tattccggtg tgtgggatac ccgggcgatt gaactgcacc
     5461 cggactgggc gcgcatcaga gaagatggca cgccggatcc gaataatacc agccgtctga
     5521 gtgcttacga gacggagctt cttataccgc agctgctgga agtcgtgcgc gattatgata
     5581 tagacggcat gtggatcgac ggcgaaaact gggcgtctca tcctgactgg agtgcagcgt
     5641 gccaggcggc ttttacaaaa gaaaccggca ttactgacat tcctaaaaaa tcaggcgatc
     5701 ctcattggct tgaatggctg gcgtttcagc gtgctctttt tgtgaagcat gtcacggatt
     5761 atacaaatgc agtgcatcag ctgaagccgg agtgcatggt tacaagcaac tggatgtata
     5821 gtgtccgcca gcctgaggag atggaagcac ccattgatta tcttagcggt gactttgatc
     5881 cgtctttcgg ttcagagcgc gccatggcag aagggcgttt tatctccagc cgcggcaagc
     5941 cctgggatct tatggcgtgg agttttctgc aaattcacga tcagggctgg actacgaaac
     6001 ccgctgtcca tttgtgccag gaagtgtcta ccgtactggc tcagggcggt gccattttta
     6061 tttatgatca gcctcagcgc tcgggaaaac tcacggactg gcatcaggat atttttgcag
     6121 aagtggcgca attctgcaga aaacgacagg ccttgagcca tcggacggaa agtattccgc
     6181 aggttgttgt tctgcacagc gagaacagct actatcgcta caattcgcct ttatacaact
     6241 tcggcggggc gaatgctgca atggaaggag cactctttgc gcttctggag aacggataca
     6301 gtgtggatat aatgaatgag gcaacgctgg gcgcgcgtct ttccgaatat ccgcttgtag
     6361 ttattcccga agcggagaat gtgtcggatg cattcaagtc aaaattgagc gcctatgtgg
     6421 aagcgggcgg ccggcttatt gtatccggtg cccatgttgc gctgcagtat ggtgatctcg
     6481 caggcgtcag cccggcttcg ggtacagcga tcgggtggct tcccgccggc aacggcgcag
     6541 tcaaatgctc cggttcgtac cagaaaacag ttctgaacgg cgcgcgtgtc ctccatcctg
     6601 ttttagaaaa ccagagtata ccggccgatg acaaagagct tgtgcccgca tccacgctga
     6661 accaggtcgg gcagggcagc gttgcggcga ttcacgggcc gattttcaga aactatttcc
     6721 agggccgcta tcccggtctg cgccaggtaa tcggtgaggt tgccgctgct ctggaaacgc
     6781 ccggcttaag tcgcacctgt gccccctggt atgttgaaat ggcactacgg aagaaggacg
     6841 gcaggacgct tgcccacttc gtcaacagaa gcgtttcagg gtatcagtct tcctattatc
     6901 atcttgttga acatgttcct gatgcgggca ccttttccat cgagattccg atggcggaaa
     6961 aaccgcagcg ctgttatatg tccccggatc cggtcgggct tgagtggcac tggaaagacg
     7021 gcgttctttc agcaaatatc agcgcattgc atattcacaa tatactggtt atagagtagc
     7081 gtttttcaga tcgtccaatt cagactgcat ccggtgcagc acaggcaggg caataccgct
     7141 ttttgcatcc agatcgtaat caagcacttt cggtgcgaca ggatgctggt tgcgcttcca
     7201 ctggcttgcc gcaaaacgct ttccgaagcg tgcaatccac tcccggagcg tttccggtcc
     7261 atggtccggg aatttctcgg caagcatgcg ccagcacaag gataaagaca gtcttttccc
     7321 gatatgcagg tgaatgattt cgtcaagaac aggataaggc atgagctcgt cttcgtccgt
     7381 ctggtcagga gcaagttcag cgctgggctt cagcgccagt acttttttca ggctctggag
     7441 cccgtccctt tcttcgagat aagcaaggag gcgggagacc agggttttcg gcatattggc
     7501 aaggggcgaa taggcgccct gattatctcc tccggcagtg ctgtagccta cagcgacttc
     7561 gctcatattg gtagtgacca gcactaatcc atccacatta tttgcccagt tcagcatgat
     7621 gtcgccgcgt atgcgtgcct gcaggttctg cctggtgatg gggcgtacct tctttttaga
     7681 ctccatgatt tccacggctt ttgaaaaagc aatatccact tcttcatgga tggcaacaac
     7741 acgaaatgga atatgcagtt cttctgccag agcgcgtgcg gcatcgtgtc cggctgtact
     7801 gctgagtgcc cggttgggca ggtaaagggt ctgaataaaa gaggcgtagt catcggtgcc
     7861 actgtctttt gcaagcatac gcgccgcctg aacggctgca agaaggcaga gagcgctgtc
     7921 ctgtccgccg gagagggcga gaaggatttt ccggaaaacg cctgctttcc gataataatc
     7981 gcgaatcccc agcgcaatgg catgtaaaat ggcatcgaga tcctggttgc tattattcgt
     8041 ttcgctcttg cgacccgccc gctctgcctg agctgcgatt tgtttggcat aggcacgcac
     8101 tgaagcgggc ttaaacgccg cttgagcgca tgaaatgccg atacaacagc cctccgcgcc
     8161 ttccagttca agagaaacgg gtaatagagg aggagtgcgc tccagttcac caagatccac
     8221 aatgacggta ttcagtgtcc attgttttgc ggaaaaggca cgggtctcct ccaggattcc
     8281 ggagggtgcg gcgatcagtc cggtgccgtc aaaaatcagc ctgccgtcgt cggttccaag
     8341 caggttggtg tggacgcaca ggccatgaag acggcgtgcc gtatccagca tgcgtatccg
     8401 acggcgctct gcataaccgg aggcgaaagg cactgctgcc atgcagcaga tgactgtcgc
     8461 acagccatca ccatttagga aagacggtgg ggatacaagc gtgtccgtgt cgaaacttgg
     8521 cagcaatgta ccccagggca tatcaaagga gagctgtccc gccgaaacgc cattaagcaa
     8581 agaatcgatc cgggaaagcg acgggaaagc ggcggcggtt tcccgcagta caattcctga
     8641 aataatttta tcgcttatga cggcggcgcc gctgcgcaaa gaaccatgat ataagacagg
     8701 gactcctaca atggcggtca ttccgtcaca ggcggccgca atctccgcca gcgatgccca
     8761 gctttgccgt gcggtatccc gccacattcc aagactctgg agattgtagc cggaaacagc
     8821 cagttcggga gcgatcagca gctggacgcc cgccttgccg gcttcctcaa tagcatccat
     8881 cagaagtgct gtgttgccgg agaagtcgcc tgtccgaact gaaagggaaa ccaggcctgt
     8941 cttaatcaat ttcatagcct acgaactccg tgcctttccc gtatgccgcc gtaaagcgat
     9001 tgcatctatt aatgcatgag cagcaaatca tgtttgagat cacccattgc cggggacaga
     9061 agtatcgggt aacgttccgg attgcgcagc cgcttgaatt cttctgtcag accgctttgc
     9121 tgagcggcgg cgtactcccg aatttcggct gctgtacgct cggcggagat ccgttttccc
     9181 agcacaaaag cctcacggag gaggggctgg gactgcgcgg cgttttcaat gcgccgggga
     9241 acctgtggat gttttcttga tcttccctct atgatacctc ctgtttccgg agtctcatat
     9301 tcagaataca tgacatcgcc cagaatatag ccttcatcgt tcaggtatcg ctgcgggtat
     9361 ttgcgcccgg ggtctgttgc tttttcgtag ttgaacgaag ttttgatcac aggtgaccac
     9421 ttgccactgg tgtctttcat tgctaccgct ttgtatacgc ccggcaaagc aggcgcatca
     9481 tacgccgtga ccagttgtgt ccccacgccc catgcattaa tccgtgctcc ctgtcgtttc
     9541 aggtcggcaa tcagatcttc attcaggtca ttggaagcaa cgataagcgg gttttccagc
     9601 ccggcctgaa gcatcatgga atagcagatt ttactgagtt ttgccagatc acctgagtcc
     9661 agacgaaccg ccggccggat aggaacaccc tgctcccgca actcgcaaaa agtacgaatt
     9721 gcattgggaa ttccgctttt gacagggtcg taggtatcga ccagaaggat gcatcgctcc
     9781 ggataaagcc gggcaaaggt acgaaatgct tccagttcgg acgaaaaact cataacccag
     9841 ctatgcgcat gggtgccggc tacaggaata ccgtaaagct gcccggcaag cacattggag
     9901 gtcgaatggc agccgccaat aaaagcagcc cgggaagcac tgagtccgcc gtcgggacca
     9961 tgggcgcgcc gcagcccgaa ttccatcacg gggtcaccgt cggcagctag gcagatgcgc
    10021 gatgctttgg tggcaatcag ggtctggtag ttcacaatat tgaggagcgc cgtttccagc
    10081 agctgcgctt caagaatagg accttccacc tgaatgaccg gttcgaaagg aaacacggca
    10141 gtcccttcag gcacacatcg aatggtacat tttttttcaa agtggcgcag atactccaaa
    10201 aagcagtcat cgaaaatttt cagagagcgc agatattcca aatcgctctc agtaaaataa
    10261 agatgctcaa aatagtgcag gaactcttcc acgcctgcag cgatggcata gccggtattg
    10321 ggaggcaggt accggaaaaa gtattcaaaa ctgagttccc ggtccacccg ttcttctttc
    10381 caatgacctg ccatcatggt cagctggtaa aagtcggtaa gaagggcaag gccctctctc
    10441 tgaaaccagg ggttatgcag tgtattcatg aaaggtcttc actatcaatc agctcgactc
    10501 cggctgcctg aagctcagca tagacggcgg cttcattttc aggaaatacc gcccgcgtgg
    10561 cattgcgtat aacaaatgct ttaaagccgt tttgaagggc gtccagagcg gtggccttga
    10621 cacaaaaatc cagcgcaagt ccgacgacaa aaacccgttt ggtttttcgt cgctccaggc
    10681 gcgtacttaa atcggtgccg tcaaaggcgc tgtatgcatc tttttcagcc gtggttgcct
    10741 tgtcaataat ggtggcatcc acagggatgc gcatatctcc gtgaaactct gcaccagggg
    10801 agttctgaac acaatgagac ggccagcacc cttcgtcaaa atggggattg tccctgaaac
    10861 tgcaatggtc attcggatgc cagtcacggg aaaagtagag ataatcaaaa aagcgctgca
    10921 gcctgttgat cggatcaata atcagttcgg catccggcac aggaagagag cctcccgaac
    10981 agaaatcatt ctggacatca acaatgatca gggcatccgt atcgtttaca tttaccataa
    11041 atttaatcct gactgaaatt aaaatgagcc aaaacaaaag cgaggatggc cgcatggtcg
    11101 aaagcaagcg gagggagtgc attcaccgga aaccattctg ccgccgcagc gtcatcattt
    11161 gcgcaaagcg cttctttgct taaaagttct cccgcaaaga caactgaaat tgttcgcccg
    11221 cggggatcac gccccgggtc gccaaagcaa tgcagttgtg ttagtggaac attgcgcagg
    11281 cctgtttctt cctgcaattc acgcatcaca gccttgtcca ggctttcgtc catctcaacg
    11341 aacccgccgg gtaatgccca acttccttta tacggttcat tttttcgccg tacaagagcc
    11401 acgtgttttt ctccggaccc gtccgcaaag acgacagcgt caactgttac catcggtcgc
    11461 ggataatcat aagaaaaaag agtcaagagc aatacctgtt tctgaaaatt tcctgttgtg
    11521 agatttcttt gtcataagca acagaacgca aacgaacctg ttttctccat aatactttat
    11581 ttagcggttc tgctgcacat ttctttaagg tattcagagg gtacgctcag gcaaaaggtt
    11641 tttgagcccg ctggatttca aaggttctta cgagcgaaag aatgtcttcc gccttaaaag
    11701 gttttaacag ataaccatca gcggcactgt cccgcatttg cggcatgctc tgtgagatcg
    11761 agcgggctgt tacgatttgc actttaatgc cggacagaac gggatgactt ttaatatatt
    11821 tacacaagtc ccagccgtcc tgatccggta gcgcgatatc cagcagaaca agccagtagt
    11881 cgcctttttc cagcagctgc aaagccccgt caccggaggc ggcgatatca atttggaagc
    11941 cttcgtcgga aaggcatctc tgcacaaaag tggctgtttc cgcatcggca tccacacaca
    12001 gtatcttgtt ccccagatgc gactttgtct gaaccggcgg gctgagtgct ttatgaatgc
    12061 cgctgagcag cgtgcttttc ctgaactgcc gcttaagccg tgcatcaaaa agctcctgat
    12121 aatcggcata aaggctgtca ttgtccgtaa taatcagcgt cggaatcttg aggtcctggg
    12181 cttcagaaaa aaactcttca agcagaacgc tcgcaagcgg tccggtttcg ctgtcaataa
    12241 tgatacagtc agggctgtgt ttctgtgcca atgtgaccgc agcagagggg taggcagcat
    12301 gaatgacatc catgccttca gagatgagct gctgccgtat ttgtgcacag agagagcgat
    12361 cctgagaaac gagttctatg agaagatggg tttcgatggg aaggggtttt gttcctgttg
    12421 actctccgcc actctgagga tgatgaaggg gcagagagaa tctgaaggta gttccttcac
    12481 ctggcgtgct ggaaaccgtg atcctgcttc cgtgcagccg gacaatgtcc tgtgcaatgg
    12541 agaggccgat ccctgttcct ccgtattttc tggttgacga agcatcatac tgatagaacc
    12601 gggtaaagat tttatcctgc ccgtcttccg ggatgccgat gcctgtgtca atcacagcaa
    12661 tgagaactgt ttctttccgc acctcaacat cgacggtcac gctgccttcg tttttgttga
    12721 attttacggc atttgacaga agattattga agacctgccc gagatttcct ttatcccctt
    12781 ccaccatgac aggtgggcgc gctgtgtttc cctgctcatc gtgtacggtc agatgcaggg
    12841 agatggcgcg cacctcagcc acaggcttga cgacctgcat acttgctctg atgcagtcaa
    12901 tgagatcaaa gctggtgaag acaagcccct cagtactgcg atgtattttg gcaaagtcga
    12961 gaaggttttc gatcagcgtg accagttttt caacattccg aagaataatc tccagatatt
    13021 ccttctgtgt atcattgatg atgcctgctt tttcattcag aatcatatcc gcataaccca
    13081 tgaccgcaac cagaggcgta cgcagctcat gggagacgtt ggacaggagg tgcgttttca
    13141 tctcgtccag tgatttcagc tccttgttgg cactgtccag tttttctttt gcggagttga
    13201 cctgatcgag ggtgattttt agattttcca ggagctgggc attacggatg gcacctgcca
    13261 gatggggcgc aatgctttca aaaagatgca ggtcgtgatc cgaaaatgcg ccgatatcat
    13321 tcgaccccag ctgaagcgat ccgaggatct gctgttccgc ccttaaaggc acgcacagaa
    13381 cgctgcagat ctccggtggg aactggcatg ccataatttc gttttctttt ttgatgtttt
    13441 caatattcag agggcgcccc gctttggcaa cccagccggc gcaactgtgg tcatcatcca
    13501 gccgggggaa acgttctctt ccgtcctgag gctccggcca gatctggcgg gtctcgtggg
    13561 cgttggcgtc tagcgtgatg gacactgccg cataatcgta atccaccaga cggtggagtt
    13621 ccgcaagaag gctctcatag acgcgtcctg cttcgacgct ggagttgact acattggcaa
    13681 ttttgttgac agtagccata cgttcagcac gctcggcaat ctctttttcc atccggaggc
    13741 ttcgtgttac atctctgaag ctgccctcaa ttccctgcat attgccctga tcgctcagga
    13801 tacgatttcc cgaaatttcc acacagaccg gctcgtcgct gaatttactc agccacacgc
    13861 gcatactgtc cacatagggc cgtcgcttga tgttttggat gaacttctgg tagtcgtcct
    13921 catccacaaa aaaatcaccc agagggcgcc ctaatacttc atcccgccag gaatagccca
    13981 gaatggcaac acctgccgga ttgataaaaa taatctgcat ttcctgattc gtctgaaaaa
    14041 tacagtcacg caaggaagtg atcaggtgcc tgtatttttc ctgtgatgcg cctaaacggt
    14101 gcatattgct ccggagcgct gccgccatct gattgaaaga agcggcaagt tcttcaatct
    14161 catcccccgt ctgaattttc agtttcagat cgagatcccc ctgccgaatg atctgtgcgc
    14221 cttcattgag aagagacagg ggacgcagaa ttttgttgtt gacaattctg taggcgatgg
    14281 cgcagaacag agcgatgatg aggcagcttg cgagcagggc gacgcctgcc gccatgttga
    14341 tggtccgaaa taccgcaggt gtcggctgat gtaccgccac ataaagggga tggtaacttg
    14401 caagcctttg gtacgctatg agcacagggg tacgtacgcc ttgcgaaaga taatcccaca
    14461 gaacgaaagc gtcctgtgtt ttttccgtgt ttttgagacg ttccgccagt ttcggatgaa
    14521 cagcagtgac ccgtggcgga gggggggaag tctcggagtc atcattcaat gacacagtaa
    14581 attgtgtgtc ttctgccatg tatatgattt cgtaccgatc ccggcggcgt ccttcaacaa
    14641 caaaattgtc gtccagaatc agttccatca gctcctgagc gctctggttt tccacgagat
    14701 atccaaggac aatttccttg ttcgcatcaa aaacaggcgc taccagcagc gccgtatagc
    14761 gcccctgaga attgaggtaa tcgggacgga gaaaacgggt ctccgtgatt ttatccagcc
    14821 cgggcagcgg cggcggctct gtgtcgtcaa ggctgaaaat agcctttcct gcagaattat
    14881 aaagataaaa cgaagaatgt agcgtgggac tttcatgcac ctcctgaagc agccgcctgc
    14941 gcaaggcctg atcagaccct gtttcactgc ggtaggcatg gcgcagccag tctacaatct
    15001 caggatactg ggcgatgcgc gtcgtgttgc gctcccgttc ctgaatcacg aggctgacgc
    15061 cgccgaccgt tttcctggct gcagtcagaa gattctgcat aacagcgagc tgctgcccct
    15121 ctcttgccgc aaagtaaccg ataagcagca ccataatcat ggggataacg ccgacccaca
    15181 ggatggaaag aatgaaacgg cgttctatgc tcaggcgaac agatgattct tccatgcgta
    15241 acttcccgaa aaaaagccgg cacatcaacc tactgaagtg ccggcttgat tatagcaatt
    15301 ggctgggcag aatgaaattt aatcctgatc gccctttctg ctgattaact cacgaacttt
    15361 cgatagcaga tgatccactt taaagggctt ggcaagataa tcatcagcgc cacatgcgaa
    15421 aacgcgctcg atgtcctgct ctttatcaag acaggtgaca gccatgatcg ggatgttgcg
    15481 tgtcgcctcg ttgttgcgca gcgcacggca tacatcaaag ccgtttattt cgggcatgag
    15541 gaagtcaaga agaataagat ccggaatggt ctgtgccgct ttcagaccca cttctgtcgc
    15601 attgctgacc ataaccagat tatacccttc gtctacaaga atccgttcca tcagcgccag
    15661 agcgtcctga tcatcatcaa cgatcataat gtgccgggac gtgaaggcct tgtcccgggg
    15721 aaacatatcg tactgctccc ggaactgatc cagatcaggc tcaagaatac gatagtgccc
    15781 gcctggtgta cgggaggctt tgatcagccc ctgtttaatc cagtttttga tgctatggtg
    15841 tgtaacatgg caaatctttg ctgcttcaaa ggtagtgtag gctttgtgtt ctcggtccat
    15901 aatgctttac ttcctgttgt tgcttaccca caatgtaata tcaattcacg cgtgcctgcg
    15961 aagtttctct cgttaacaca gaattcacaa tttaccacgc ttccctgcat attatatata
    16021 aaatacggca agactatcaa atgtttttta ctgcatgagt gatgattata caaatcgtgc
    16081 cataagtgaa aaaaaggcag ggtttttcag agtaaagaac ggatgttcag gatgcgcctg
    16141 cttggccctt cgccgtcagc ctgatttcag aggcaggcgc cgggaaagag taacaggaga
    16201 tattccagaa attctataac gttttttgtg aacgctccgt tcgtatctct gtattcagaa
    16261 aataatctga aaaaggtgaa tatatgcatg cccaatatgc tatttatgcg caggaaatga
    16321 tgagcatctc aaaatgcccg caccgtatcc gttcttcctg aacgaaaaga gaccgcaaaa
    16381 caggcagatc tgcagccgaa aggcttttga gatgggcgtt gcaaaaaagt cttagtattg
    16441 gaataaaggt gtggaaaggt aacgttcgcc agtatccggt aggatgacga caatggtctt
    16501 tccttcatat tcatcatttc ttgccagacg caaagcggct gctgcggctg caccgctgct
    16561 gattccgcag ataatgcctt cttcctttgc gaggcgccgt gccatttcaa aggctttttc
    16621 atccgaaatc gtttcaattt tgtcaatcag actgagatcc aggacgtccg gaataaagcc
    16681 tgcgccgatc ccctgaattt tgtgtttccc cggcttttcg cccgataaga cggcagaagt
    16741 gactggctcg acggcaacgc attcaatctg ttttttcttt tcttttttga tataacgccc
    16801 tattccggta atggtgccgc ctgtgccgac cccagcgacc agtacatcga tggcaccgtc
    16861 tgtatcttcc caaatttcag gacccgttgt ccggaaatgg gcttcagggt tggaagggtt
    16921 tttaaattgt tgtggcagga aaaaatcagg gttttcttct gcaatctgtt ctgctgtcga
    16981 aatggcaccg ggcatacctt tttcaccggg ggtcagcacc agttcggcgc cgaatgcttt
    17041 cagcatggca cgccgttcga gactcatggt ttccggcatg gtaagcacaa gccggtaccc
    17101 ccgggcagca gcaacaaaag ccagagcaat accggtattg cctgatgttg gttccacgat
    17161 ggttccgcca ggcttaagga gaccgcgctt ctccgcatcc cataccattt gtgcgccgat
    17221 acggcacttg acggaagaag caggattgcg gccttctact tttgccagca catgagcggc
    17281 tttggaatct gctgtcactt tgttgagccg gacaagcggt gttttgccta tggacaggct
    17341 gttgtcctga taaaaagcca tgatatactc cttcgcaggt tgtaaagttg cacaagttcc
    17401 gctttttgcc acaggatcat ttggcatttc tttgaaaagg gctattccta ctatatcact
    17461 tgtattagta gtaatgcaag aaatagagtc cgattttttc cgggacttaa agttgaacgc
    17521 cattccaagt ctctatataa ttggatcgga ggtgcgtgag ggattcgctt caatgaacag
    17581 caaaatgtcc gtttaccaat atcggtacaa aagaggaacc agtcatgatt aaagtaggta
    17641 tagtaggagc tactggctat ggcggtcgcg aactgctccg gcttttatgt gcgcatcctc
    17701 aggtgaccgt tacagctgct gcctctacaa gtgtttccgg agatgcactg gcggatgagc
    17761 tgccagcctt tgcaaaattg atcgaccttg aatttgaaac ctttgatgcc acttcgctgg
    17821 cggctcgctg cgacgttgtt tttgtcggtg tccccggcaa agcgagcatg gcgcctgtgg
    17881 ctgctcttca cgaagcaggt atatgcgtga tagacatcgg gcctgatttt cggttgcagg
    17941 atctggcgct ttttcagaaa tactacggtg tagagcacat agcgcctgaa ctggtgcaga
    18001 aagcggttta tggctttcct gcctggtacc gtgatgcgtt gcgacaggcg gagattattg
    18061 cggttcccgg ctgttatccg gtcagcatgc tcacgccgct gcgccccctg accgatgcgc
    18121 ctgtttcggg agttccgata gtgatagaca gcatttccgg gatttccgga gccggtaaat
    18181 ccctgtctca agcctttcat ttccctgaga tgaatgaaaa tctcaaggca tataaaattg
    18241 gcgtacacca gcacattccc gaggtggagc aggaattgct gaaccgcttt acaattcaat
    18301 tcacccccca tgtaggtcct ttaaccagag gtattctgac gaccatcaca ttgcgcccgg
    18361 aaacactttt cgatccggcc ccttattatg cctgctatgc tgatgagcct tttatccgtg
    18421 tgctcggtgc cggaaaaaca ccggacatca accaggtgcg cgcctcgaat ttctgcgact
    18481 tcggttgggt ttgcgatgag agaaccggca atatcgttat ggtcagcgca atagataatc
    18541 ttatgggcgg cacggcaggc atggcggttc agtgcatgaa catccgcttc ggtcttgagg
    18601 aaaacaccgg actgaagtgg gcggggatgg caccatgaag aaaatatcgg gaggtttatg
    18661 cgctcctgcg ggattcaggg cgggagcggc ggcagccggt gtgaaaaaag ccaacaccac
    18721 ccgtctggat tgtgccctga ttgtcagtga cagaccggcg tcggtagccg gtgtttttac
    18781 gaccaatcgt gtttttgccg ccccggtgcg gtactgccgt gagatatgtg cccgggggcg
    18841 tgcccgcgct tttctggtca acagcggcaa tgccaacgcc tgtaccggtg agtcaggcta
    18901 taaggatgtg ctttccatag ctgcctgtgc gggtgctctg ctggatattc ccgccgatga
    18961 aatctgtgtc agcagcaccg gtgtaatcgg tgttccactg cctcttgacc ggctcagcgg
    19021 gggcatcaat cagtgtgccg catcgctttc ttccgaagga agcgccgccg ctgcccgggc
    19081 gatcatgacg acagatacac gcccgaagga gatggcactg gaaatagcgc tctccggcgg
    19141 aacggttcgc ctgggcggta tagcaaaagg tgcgggcatg attgcgccca acctggcgac
    19201 catgcttgct tttctgacga cagatgccgc tcttgacccg cctctgcttc ggcgtctatt
    19261 gaaggaagcg gcggatgttt cgttcaattg catttgtgtt gacaatgata tgagcaccaa
    19321 tgacacgctt ctgattatgg caaacggcgc ttcaggcacc ccgctgttga aggaaggcac
    19381 ggctgacagc cgtctttttg cggaagcgct tcagcggctc tgcgccgatc ttgcccgtga
    19441 actggtgctg gatggagaag gagcgacgaa gttcgtgaca attgccgtgg aaggagcaaa
    19501 agacactgct tccgcacggc agatggcagc caccatcgcc aaatcccagc tgtgcaaaac
    19561 agccttttac ggcagcgatg ccaactgggg gcgtattgtg tgcgcagccg gttattccgg
    19621 tgtggtattt gatccatccc gtacagattt atatattcag ggcgtccatg ttttcagcaa
    19681 tggccggccc cttcctgaaa aggacagcga agtgcagcct ctgatggcgt cgcctgaaat
    19741 tacagtgcgg ttgtgccttc aggaaggcac cgcttccggg gaatactgga catctgacct
    19801 gagtatcgat tatgtaaaaa tcaatgcaga ttatcgcacc tgagcaaagg atccaactat
    19861 gcaggagctg attaaaaaag cagaagtgct catagaggca ttgccttata tccgtcagtt
    19921 tttcgggaaa acagtcctca tcaaatacgg cggtgcggct atgcttgatc cggcacttcg
    19981 cgccagtacc attcaggatg ttgtgctgat gcgttacgtc ggtatgaacc cgattatcgt
    20041 tcatggcggc ggtccggcga tcaacggcat gctgaaacgg cttgatattc agtcgcattt
    20101 cactgcccag gggctacgtg tgaccgacag cgccaccatg gaagtggtgg agatgatgct
    20161 ggcgggttct gtcaataaag atattgtaaa tctgatcaac tgtgcaggcg gcgaggccgt
    20221 cgggctttgc ggtaaggacg gccgtatgct ctatgcacgc aagaaactgg ctgaaagcgg
    20281 cgaagatctg gggcaggtgg gtgaaattac cagtgtcaat acccgtgttc tgacagcgtt
    20341 gtgtgaaagc ggaatgatcc cggtggtggc acctatagca accgatgagg atggcggcac
    20401 ctggaatatt aacgccgata cggcggcagg cgatatcgct gctgcagtca aagcggaaaa
    20461 actggttttt cttaccgata caccggggct gctccgggaa gtgaagaacc cggagacgtt
    20521 gataaaacag gttcacatca gcgaagtcca gcggctgaaa gaacagggta ttatagccgg
    20581 tggcatggtt ccgaaagtgg aggcatgcat caaagcgctg aaccacggtg tacggcgcac
    20641 ccatattgtt gatggccgtg ttcctcacag catgctgctt gaaatcttta cggataaagg
    20701 aatgggatct ctggtcaccc gcgatacggc ggtcgtgcag gaaggaaact gagagcagat
    20761 ggatacagaa agctattttg taaatatcat tattcagggc ggcattctca tgtggcccat
    20821 catgatgtgc tctgtcatcg gccttgccat agtgatagag cgtttttttg tgctgcgccg
    20881 tgccgatatc gacacccgtg aatttatgga tgttatgcgg caggtgctgc gccagaacag
    20941 aatcagggaa gccattgaga tctgcgatga gaccgaggcg ccggtggcac gcatcttaaa
    21001 agcgggtatt cttaagcatg accgcagtaa agacgatatt cgcgaagcca tcgaagaggc
    21061 gggccgcttt gaaattccgc gtctggagcg ccatctttct gcactggcaa cctgttcaac
    21121 catcgcaccg cttttgggac tcctcggcac ggttcagggt atgattaaag ccttcagcca
    21181 gatccagaac aagcgcggtc aggtgaaccc cagcgatctg gcggaaggta taggcaatgc
    21241 gctgatcacg acggcggccg ggctcacagt tgccattccc attcttgttg ttcacaacta
    21301 tttcattaca cgggtggaca acatggttct cgaaatggag ctcagctctt ctgaactgat
    21361 cgagctgctg acgcagcacc acggtgatcg agagatataa gcatgaaatt caaaagagat
    21421 aaccgcagaa aagtgctcgc ttcgctggac atgaccccgc ttattgacgt ggtctttcag
    21481 ctcctgatct tttttatggt ttcttccact tttgtggtgc agtcttcggt gcccattgag
    21541 ctgcctgagg cggaaggggc ggatcagctc gaaagcaaag aaatgtccat tacccttgtt
    21601 tatggtgccg gcggtcccga ggacggcggc gaaatttttg ttaacgatat ctccattacc
    21661 aattggacac aattgacgcg gtctctggta gagctgcgcg aacgggatcc tgaagcgctg
    21721 gtgctcatcc gtcctgatgc acgggtgccc acagaacgtc tggtcaaggt gcttggtttt
    21781 gccaatagtg tgggcataaa aaactacggg attgccgcca cttcgccaac gtctgaaaga
    21841 tgacccggca ccgccgggac agggcttttg ccatgacagc cgaaaaagca gtatatccgg
    21901 atcctatcgc atatcggcgg cggctgcagc gaaaaaaccg gaagcggctc ttttgggcag
    21961 caggcttttc tctccttttt cacctttcca tgattacttt tttcgaaatc ggtgtgcgtt
    22021 atccgcggga cgatatccgt ttctaccagg tgcagattgt gtctgttccg gaagtccccc
    22081 gggctgccgc tccggcggcc gcttcttctg atgccctgag tttgagcggg agaaatgact
    22141 atggccagct cccgctgatt gccttgcctg ttgtcgaatt tgctgaaatg gaacgcctcc
    22201 gggtacgtta cgatacggcc gcgcccctgg cagaattagc ggagcgcccg acgcagcttg
    22261 tttatacgga cagctgggcg cgtttcagtt ctgagctgca gcgcatgagt cgatctcttc
    22321 gggaccttgc cctttctgaa gaaggggctg tgccacggga aaagcagccg aaagaggcac
    22381 agcggcttta cttcagaccc gccgaaggtt tcgaagccta tattgaatgg acggggccgc
    22441 ccgctgatcg tgccctgctg ttttcaccgc ccatgaaagc cctgtggaat gtatcgactg
    22501 cactgaaaag ccgtcaactg gaaattgtgt ttcaggttga tcccgggggc agagttgtga
    22561 acgtatggag tcctgaagtg gaggatgcgg cggtggttga cgatatccag atgacgctgc
    22621 tccaataccg tttttccccg ttgcccgaat tggggatgtc aggctccggt gaaggagaaa
    22681 accgcagcct gaaagaccag tccggcgtgc tgttcattaa agcgtcggag caggcgccat
    22741 gatacaaatt acgttgggaa cagcactgct tgtttacggc accgtaatta tagtcgggtt
    22801 tgccctcctg tcacttctgg gtgaactacg tgctacgggc gtttatacgg ttctggaaaa
    22861 acagttcatg tggcgttgtg ttttctgcag tttcctgtat ctggatcagg aagcggtgac
    22921 aatttcccgg tgtccccgat gcggcagtta taacaagtcc aaagaagcgc aggacgagcg
    22981 tgtgagtgcg aaccgtgaac attgggcacc ggagccggtt tccggtccgg ctgatccgga
    23041 agaccttccg cgccgcaatc catcgaggag aaaacgcccg ggtcagtccc ggcgcggccc
    23101 ccgtcgtcgc cgctgattct ccccgtcctt tttccctttt ccatcctgcc cgcagcagaa
    23161 ttccgttttc taaaagaaaa gcctctgccg gaagaggccg gcagaggcga ggggaactat
    23221 agagtggtga ttattttttc ttgcagcact tcttggcgtt aggcttgctg caggcgcgcg
    23281 gtgtttcaat ggcggcctga gcagcggcaa gtcgtgcgat aggcagacgg taggggctgc
    23341 agctgacgta attcagaccg acccggtgac agaatttcac ggaagaaggt tctccgccgt
    23401 gctcgccgca gataccgcat tttagatcgg ggcgtgtgga acggcctttc tgtacagcca
    23461 tatcaaccaa ttgtcccacg ccttcctgat ccagggctgc aaagggatca tcatccagaa
    23521 ttcgtttttc cagatagatg ggcaggaaag tgccgatatc atcgcgcgaa taaccgaagg
    23581 tcatctgggt cagatcgttg gtgccgaagc tgaagaagtc tgccgattcg gcaatcttat
    23641 cggcgatgag cgtggcacgg ggcacttcga tcatggtacc gatgagataa ttgactttcg
    23701 ttttcgtctc tttctgaaca tcagcggcga ccgcttcgac gatggcgcgc tggtttgcaa
    23761 gttcttcgcg ggtaccgatg aggggaatca tgatttccgg gagaacatga attttcttct
    23821 tggtgagacg ggcagcagcg gtgataatgg cgcgcgcctg catttcggta atttcgggat
    23881 aagcaatgcc gagacggcag ccacgatggc cgagcatggg attgaattca tggagcgcat
    23941 tgattcgttc aactacctgg gcaggcttca gaccggcatg gttggccagg gcttccagtt
    24001 cttttttcgc acccggctca agcagactgc cgacaaattc atgcagcggc gggtcaagca
    24061 gacgaacggt aacgggcttg cccttcatgg ctgtgaagat ttcgacaaag tcctgggtct
    24121 gatagtccaa aagcttcatg acggctttgc gtcggtctgc ttcgttgtcc gccaaaatca
    24181 tttcacggac gtagatgata cggtcggcgt cgaagaacat atgttcagta cgggcaagtc
    24241 cgatgccttc agcaccgaag cgcagcgctg cagccgcatc ttcctggctt tcagcattgg
    24301 cacgaacgcc gatctgacgg cgggcatctg cccatttcat cagcttgctg taccatttgt
    24361 tcttttcagg ctgcgccgga aggacaggaa tctgccccag ataaactgtt ccggcagtac
    24421 cgttcatgct gatccagtcg ccttctttca ctgtgatatt gccaacagtg aatttctttt
    24481 cgcgggcatt aatggtaacg gcggcgcatc cgacgatgca gcatttaccc cagccacgcg
    24541 ccaccagtgc tgcgtgggag gtcatgccgc ccatggaggt aaggatggca acggaggaac
    24601 gcatgccttc cacatcttca ggggaagttt cattgcggac aagaatgact ttttcgcctt
    24661 ttgccgccca ttctttagcg tcttcactgg taaagacgac gcggccaacg ccgccgccgg
    24721 gaccggcggg aagggctttg gcgatgtact tctctttttt ctccgcctga ggatcaagca
    24781 tggggtgaag aagttcatca agctgttcag gttttacacg catgacagca gtgtcttcag
    24841 taatcagccc ggattcacac atttcaacgg ccatgcgaac cgctgcaccg ccggtacgtt
    24901 tgccggtacg ggtctgcagc atgtaaagag tgccgtcttc aatagtgaat tcaacgtcct
    24961 gcatatccgt gtaatgctct tcgagcagat cgcggatgtc ggcgagctgt ttgtaaaggg
    25021 caggccactc tttctgcagc gatttcatgt gtctgttggc gtcggttttg gtggcatcat
    25081 tcagcggcga aggggtacgc gtgccggcga ccacgtcttc accctgtgcg ttgaccagcc
    25141 attcaccgaa gaatttgttt tcgccggtgg cagggttacg tgtaaaagcg acgccggtgg
    25201 cagaactgtc acccagatta ccgaagacca ttgcctgtac gtttacggct gtaccccaat
    25261 tatcagggat gccttcaatc cggcggtagg cgatggcacg cttgccgttc caggactgga
    25321 atacggcctt aatgccgccc caaagctgat ctttgggatt gtcggggaag tttttgccaa
    25381 aacacttttt gacaatgact ttgtattctt cgcaaagatt tttaaggtct tctgcatcaa
    25441 gatcggtgtc ttctgttacg cctttggctt ccttcatggc atccatggct tcttccatca
    25501 gatgacggac gcctgcacca tcggcaggtt caataccggc ggctttttcc attacgacgt
    25561 cggcgtacat catgatcaga cgacggtagg agtcatacac aaaacgagga tcgccactct
    25621 tcttaatcag gccgggaagc gtatttttgc tcagaccgat gttgagtacg gtatccatca
    25681 tgccgggcat ggacatgcgg gcaccggaac gtacagcgag aagcagcggg ttttcggggt
    25741 cgtcgaattt tttcttcatc actttttcga cttttgccag tgctttctcg gtctcatcat
    25801 aaacgttctg cggaagtttg cctttgtttt cataatattc ggtgcaaact tcggtggtaa
    25861 tggtgaagcc ggcgggaacg ggaatcccca tgctcgccat ttcgcataga ttggcacctt
    25921 ttccaccaag caggtttttc atttcgcctg tgccttcggc tttgccaccg ccaaagaaat
    25981 acacactctt ttttgccata acagtagact ctccttgggt tgttacacga gatccccttg
    26041 tggtgctttt gtagcgcatg tacaagaact gcggaaaacg gggatagctt agaaggtcca
    26101 gcgagttgcg ccgcgaatgc ggtaaacacc gtcagcattc tactacaaat acggttcaat
    26161 atgcaaatgg tgggcggatt caaggctgaa atgcaattat tgaattgaat aagacttcgg
    26221 caggcgt
//
LOCUS       DAOPWB010000032        25902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1879_length_25902_cov_5.395791, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000032 DAOPWB010000000
VERSION     DAOPWB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 25902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25902
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1879_length_25902_cov_5.395791"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKN92_04715"
     CDS             complement(<1..347)
                     /locus_tag="PKN92_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48127.1"
                     /translation="MMRFLPLSFLICFCFSLPFSSAAEDNTLRPGMRVAFLTAEATLE
                     EMGEHSLAAWQFLAEQPDTEILFNRDNAGFIDRSGTARELSEFDVVWYHQGDDVRDTS
                     LYRGAQRTEILEF"
     gene            512..661
                     /locus_tag="PKN92_04720"
     CDS             512..661
                     /locus_tag="PKN92_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNZ48128.1"
                     /translation="MELLKEIWAFMRVRKKFWLAPIIISLILLGLLFFFAGQAGVVSP
                     FIYAL"
     gene            729..4904
                     /locus_tag="PKN92_04725"
     CDS             729..4904
                     /locus_tag="PKN92_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48129.1"
                     /translation="MPESHLPVESPSDAGHKPLLSRTELWVLTGILIAAALLRFWYLS
                     AVMHAPDYSALQQDPEVQDHFARAILTGDWSERPGETDPEIRTTPFYRPPGYGYLLTV
                     LYFFSKGSYLAPRLLNVFLGLAAVYFLFRLGRHLFGLVAGLACALYSAFCGIFIYWEG
                     EVNDPAIFVFLAVALFSLLLRWSASFKLRYLFLLGLLFGAYGLARPNILGFGPFVALW
                     LGWIAWRHKKLRLLPAAWGTLLISTFILIVPVTVRNYVASGEFVPIATYFGHNLIIGN
                     HEQSDGVSPWLPYLQELEGTGKWAAKDYVNVVRGLAKELGRDSISHTEASAVFAQKAR
                     AYMRAHPAETIRSMVKKWILLWTPVEITCNKVVQCELNHYLPLRFLPTFRWIAALFTS
                     GMLLLLWALFRRTFNGRHQDAMPLVVLMILFVLYYLTSFLPFFVNGRARVPVFPVLIL
                     LGSYGVQCCAGWLKNRQYGKVAAYGITFLLSLMFFSVEWIPVEIDASRWHYQRADSWL
                     RRGETAKALEEGKAVITAQGKAYMHQRLGREFMALGYPNDAIAQYQAGLERDPEYQDL
                     WFLLGRALEERGDLKEAEKTYRDALTLNPFDARALNALGMLCYGQKRYEEALKLFDAA
                     LNVAPDYGDVIDHISQCMKALGRVDDGIARFKSMLAENPDQKYVHFFLGRDLLELGQS
                     EEAFTHFAEALRLDPALDGASYLWAKELSRQGKLEEAIRLHEATLKINPDLKYTQAAL
                     GSDLSRLGRTDDAIRHFSEAVRIDPSFQDARYLLGKELARANRLDEAQAQYQEAIRLN
                     PQDARAHHEMGMFFAEQGDFPEALQHFEAALKVDPEFAFALDRMGRVLSIMGRSDEAI
                     ARYKQALEVKPDNQDLCYLLGRELLAQDKKDEAERYFRRALVLNPSDARAHNELGALL
                     AAQGKKDEAMRHYEEALRAAPQFGYVLYKMGQLLAEEGNLKAAEARFKEALNLDPDNQ
                     DLYYLIGRELVSQERNDEALAYFEKALAMNPSDARAHNDYALLIGSKQPEDAIAHYEA
                     ALKIAPDFTLALNNWGNLLLELNQPDAAEEKFRRALQHRPDDEYAWYNLARVAEKKGD
                     LQAAEEAYRKAMENSPENSQIPNNLGLLMARQGRVVEAQEYYEKALSIDDENPLPYNN
                     LGQLAEKSGDAETAIMRYQQALEKKPSFLLALNNLSALLTRCAKTEDMEVWYTRAFEA
                     DPDNGDVHNSYAFYLFRLGRDEEARREYMKAIACIPVFPLALNNLGNLEFRAQNNAEA
                     EKWYKKALEVHPLDLHADFNLGRLFLAENRKEEALHMLESAAVKQSDEPRLLLETANA
                     FVAAGSLKDAIHCYKRVIELAPDNKAALFNLASVYLDGYRDAAQARPLLENLQQMDPD
                     FPGLDQLLAKMKQ"
     gene            complement(4925..5875)
                     /locus_tag="PKN92_04730"
     CDS             complement(4925..5875)
                     /locus_tag="PKN92_04730"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNZ48130.1"
                     /translation="MSATNTFYRKIRKRGSLSLPALFFLFLFFTATAFAESPEKCHAW
                     IPVFRGVDYLFIHQETPLPQRIHLVKIDLKDPAIRFTSTPPNTEKARRETRCETTLAF
                     VKEKKAQLGINGNFFINDHQEDTDLLGLAVSDGTVVSLWDKGWAKYAVNIAEDNQVTF
                     VARPEGGENTSETVPPVKLFNALTGNIMLVEDGKVKTEEEGDRHPRTAVGLTADQKML
                     FLIADGRQPGHCTGLTYHDMATLFVEFNAVSALALDGGGSSTLVLADPEPRVVNVPLP
                     ATVPEGIVLNPPGVERKNGNNLAVFAAPLESRQSGESSAE"
     gene            6007..6618
                     /gene="cysC"
                     /locus_tag="PKN92_04735"
     CDS             6007..6618
                     /gene="cysC"
                     /locus_tag="PKN92_04735"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNZ48131.1"
                     /translation="MADSIKASNITWHHATVSPEDRSVKNGHQGAILWFTGLSASGKS
                     TVAQAVQSVLFESGCQAYVLDGDNVRHGLNKDLGFSPEDREENIRRIGEVSALFCDAG
                     FIVSTAFISPYRADRDKARALCPDRFYEIFVKCPVEVCETRDPKGLYQKAREGKIPEF
                     TGISAPYEEPEKPELILDTATLSLEACVAEVIQMLQKEGILQA"
     gene            complement(6629..7888)
                     /locus_tag="PKN92_04740"
     CDS             complement(6629..7888)
                     /locus_tag="PKN92_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48132.1"
                     /translation="MPWIFRRQEKLSGHARLIIALNALYSIAEALCSVFVGVYFYINS
                     HNINTVFFHYTVLYLTTPFAFVFAGWLTRKRDRSFLFRAGLVLHALYYAALLFLRDSS
                     PEYAGMLGGLLGITWGFFWAGNNTYQYDFSSGNGGREFFLGMISAVSAGAKILAPLMS
                     GLIISFAPVPQQGYEIIFVVALMLYSVAITFSFKIPASPKSGSFPLKQALFPPRAHRD
                     WRILMLAGISLAGSFHIFHFLLALIMYFEIGNEAAVGGYVSFQGLVTVTVSYLVGRYV
                     VPQSRKRFMFWGVLVLVAAGLIVFWETTVWTLLIFGFLRSISLPLYGIPDTAVRFEVM
                     ESMMKEPGDRIAYLCAWESPLAVGRLIAMASLFVLYHLYGGGGLRVMLLLLCLIRLAT
                     YALMCRISIIRNPALSPSAGDTCPPQL"
     gene            8036..9556
                     /locus_tag="PKN92_04745"
     CDS             8036..9556
                     /locus_tag="PKN92_04745"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNZ48133.1"
                     /translation="MVEPTMAEYVLEMLDITKRYPGVMALNKAQLQLRPGEVHCLVGE
                     NGAGKSTLMKVLAGAIARDEGSIRIDGCEYDYHTPLQAAKLGISMIHQEFNLAPQLSV
                     AENIYLGRAPSSRGIIRWRQMYDMARDALAMLDSDLDPRRLVATLSVAQQQMVEIAKA
                     LSINARILVMDEPSATLTDHELASLFHIMKILHRQGMGIIYISHRLEEIFAVGNRVTV
                     MRDGCWIGTHDLCDVTREELIRMMVGRELQDEFPHRNAEIGEVRLEVQSLARQGSFYP
                     LSFSVRAGEVVGLTGLVGSGRTEIARAIFGADPPTSGAVLVDGCDATSRTPRQAIRNG
                     IGLLTEDRKGQGLVLGMSIKNNISLANMKAVQKGVFLSPSLEKAVAESYVAELQIKTP
                     HVEEIAHHLSGGTQQKVVLAKWLLTDSKVLIFDEPTRGVDVGAKVEIYRLINTIASQG
                     VAVLIITSELPEALGMCDRLLVMREGHLMGSLSIEEADQEKIMELATGDIRKAVPV"
     gene            9666..12902
                     /locus_tag="PKN92_04750"
     CDS             9666..12902
                     /locus_tag="PKN92_04750"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNZ48134.1"
                     /translation="MSKQLLLLCLFSLMIMGSIQAELSAPFALKCEYRVNPEGIDTFA
                     PRFSWKLDSQDRAQAQSAWRILVASSEEKLANNEGDLWDSGIVRSDNAIQVMYGGVPL
                     QSFQKCWWKVCVQDQDGKEGEWSESAFWSMGVLGEQDWAGAKWMGMPKGEEESAEQLA
                     LNDSFWIWHSEGSPQIEAPIGSCFFRAHFTLPNEKINQAALMLSVDNSAVIFVNGEKI
                     GKWTTYHSAGTFPIQNALVPGINTLCIEAENAGGNPNPAGMIALIQIETASGTILNRS
                     TDASWKSSYNREPGWEKSSFDDSHWKTAQVLGKNGCEPWPIALIAEDRTLPARMMRRE
                     FEVESPLKRAVVSFSGLGLSELYLNGEKVGDHVLSPGLTQYNKRIFYVTHDVTGDLRE
                     GKNALGLWLGNGRYFAPRISDPTKTETFGLPEARLVLRLEYENGSVATVVTDENWRVT
                     DQGPIRKNNEYDGETYDARMELPGWDMPGFDDSSWQQAQELEGERGALSAQMMEPIRV
                     VRYVYPKAIANPAPGKYIYDMGQNMVGWVRMKVEGPQGAVVKQRFAEVVKDDGTLYLD
                     NIRGAKVTNEYYLKGKGEEVWEPRFTYFGFRYVEITGYPGVPALDDIVGCVVHDDMET
                     AGHFRCSNDLVNRIVSNISWGVRGNYRSFPTDCPQRDERQAWLGDRSAESQGETYLFD
                     VAALYAKWVCDMEDAQRETGSVSDVSPAYWPLYNDGVTWPSSFIIIPGMLYRQYGDAS
                     VMARHYDGMKKWITHMSGYIKDGIMPRDTYGDWCVPPEEQHLIHSQDPARKTSGDFIG
                     TAYFIHDLRCMEQYASLLGKEQDCTQFKAQADRMTEAFNRTFYSSDQGFYVNGTETSC
                     VLPLAFGLVPEAARSSLFSHLVTSIMDKGQGHLSTGLIGGQWLMRALTQGGRPDVAWT
                     LASQSTYPSWGYMVKKDATTIWELWNGDTADPAMNSHNHVMLVGDLNIWLYENVAGIR
                     AAAPGFKKIIMDPVMPEGLTFAEASYNSIYGQIFSRWDKTDNEIRWRVGIPANTSAKL
                     LLPAGDPSVIIESGTPAKVAKGLHFLETDGQKCTFDAGSGWYEFAISQ"
     gene            13172..22015
                     /locus_tag="PKN92_04755"
     CDS             13172..22015
                     /locus_tag="PKN92_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48135.1"
                     /translation="MNIRTSHRVQGFRPSVSQERGAALIVALSVFLVLLSLAITFSLI
                     VRYETQMTQQATSRALAERLLDGAMAQAQYRLNRDLDLHADAMSLDHGWRTRFNGAAF
                     VGKSWAQQFQGEKAHQPLYKNAEGLICVNLEPVERVLRGMGRLADGVLYAHFLKDGHL
                     EPLFRGPRTESWLYVPRMQGNAILLYASRDEVELVNSLGEVLSPADLNNALTGLRSER
                     FAFWDDQIDPDKPNDEIFPFAVPNDLSPDIDPATGLVLSALPQEKVNQWADVDSTGDG
                     MSDAIWMPIVQDVNHFGDGIDNMLSGRPDPARSVDGEFIPLHESAPFVYHGLGLPRAA
                     RSEDLFELDQMKNLHFAGRFEDPPLGDGYDNNNDGMVDDLSENQLFLTVPLPGLMMQV
                     DLNSDGLFDERDYYEFNNYRGPLYVQLPPIIYVPVIVGMDALGEPLYDRIPLSIDAVD
                     MLDNDYDLMANNFRSYAYVGPHEKLTDASAADVYAGKQIPFSRASAAEAHAHYTAWMP
                     YKDPVEDTVGWLGPDRKDRLFSDAHHRAYHDINVDAVLNGPGALRLFIVPADAASGGG
                     VLSKVELPAGNPLAKILRQPNALRVTCSGEPVSEVIGRYAVHISDESGKVDLNAAGGY
                     YSDEISGDLPSLHLGQTLFEYNTRVLPDIGKGRSYDFWLMRTGSDDSWGTVDEFFPGY
                     HLVDDNGNALLLALKGWNYSGHFLNGKGLDIPAMSGATREYLFNNVSSPLATYEDQTL
                     RLKQRLTMLGRFDGIDEPSELQLYNPLKNREDPARSDRLFLRKEDLKRHGDVGPNRWD
                     YLKNLVTVNSEKRNTFHTRNSGKTENFYQIDPNTATPQQIATAMLFRADVTNVVDKMH
                     LPSFAEGLRQFDTAFSGDLMGKVKTGLGMGTTPFGTFFPADPILQIMRLAADITSARN
                     PNPGVVKLTTDPLPRDPTIYRKYNLFSGIANSILLPAESWNERERIADENVLPTGDLQ
                     AALTGTSGEEFDQKRLISPDTWWKQQTGEDRLIQYATSSLDSIRINEIMVRPVRRVEA
                     EMVAGAAVLPELNLNPAPVDGMLPFELERRFLQNPPENWSLRSAYDYPGHAQPLLGDR
                     TAWVYTPDDPLVPADPRDVMEFRFKESPGLPAGNYYLLANLTDQYGRMTVADVDVLEY
                     AIRYTGTGGPGVFEDFAEEASLAALFAQIGYAGTASDLQLLTSKNFQRINAPEFLATE
                     SMRMHGSASAPGWAFLPHRNAFLELSDTPNLLDDFEYYIKTKLPGFALPPDATMQDLL
                     LTFWTVVMGCPSPPADATWEALNEYATVNGCVLPPPADPNDLLDPLTTDNAWYNLLGL
                     LSRFYGIGGAYSAGGTETGYTVFIPPLASGQELVVAIRMNPVVRASYQEVINECIANN
                     DFAGIPDPVTVADWQFLQGLISAGVVLRDANDFPKPLFAINFFDFSQEPSHEYIELAN
                     ISDEEVDLSGWVLEIGVPDPPGATHDPMMKDPYKSQWEIPANIKIAPQGYLLLSADIA
                     DSTGNNHLFNSNRLGLKSDGTDAITVPPIHNQFSPDPVALPVLEPLLHDETGSVFLRK
                     DGIDYIDNDGDGLSSQLMWGVNAVPTTESDFERRDTDHVLSEMRTHGTGPGGQGIPAG
                     SRIVPLINRQLWKENSRSHPDANPTAMEKYLYKLNPFTPDQQSVNITDLKDTEEGLKY
                     LSRFLLRGGLFPNYPERDGIDNDGDGGYAVRNSIKGYVPVIPSVYEQELISYVPGTLD
                     KDMIDNNLNGMMDNRGYERIRASQNYTPSINLPGFTYDSHANPLLSEGVDEGRLGFPI
                     WYTGGILSGFQGFLPPAATGLPRFYGPGSYEQTFLPLYNLNTPAAAQDYNEWARRMAA
                     GTISFSIEDPSLGYTMTVAPASFNTGFAPVLAASDSPDWQAFVERRWNSGDNVIVTLY
                     VGHPSRSIIADQVTYTEQDVINRTKDDIVDCPYTIQGFTSYGFDGSGNLAWVPPSSAS
                     APYEPVCLAPADPGRHSFWLPDHMGLDFYRSLERKHPMYHGDRFGLANRWTATDGAYD
                     DWAESMSLFETGLGLMNLDTNDAPIAVRWPGRQAYMADHTTATGTYRQRFDQKLDPAY
                     KDHALQLYGHAFWGSPLRMNTQARLWENPLDILQVNSDVKEWLNPEASPIRNQRYYRQ
                     FAGLDAYRNTGHDLNLSINVNSLTDDPTKDKERRLSSDWAVRRAKLRKKPFATLGDLV
                     ELPCFSFEKIIEGPSQASAEYVPQYAVSVVNTHYMSTETKSSGGTGVLHYSNHLELFT
                     YGADRDLTSALLGQAVYGGGGSMHGHRGKDNQPPTLVKDTLATAAMNPVILTVGQAQV
                     VPLWPAPDSVNPSTGSVLDPADLYSLFQWKDGLPPHMWTPVYLFNLPVYGDWYEEKPW
                     QTVQYPRRYDGSSIGFSPPVTNVLVEPVYIFNGEFICRQYGLSLPDDAEWVASKWPVR
                     TEVTLQSDAAGRRQPTDRACMYVSRYIPDYGSENRPEVLFVWDKETGLENGDYMAYVG
                     TFILGMRKTIEHTKAVADNYFGRTATPLPPARPSFLYDGPTAARTEAILNLDPTHPSM
                     LKNPTAPRFDPRLAIEFITNRRDAEQMLEPVELTAVSAGSGHHGMPHPADWLSNSPGS
                     AVAGYCKADQQGYVFYSDNPSLAWRPIPVRVENNYLALRVRNLGDPNQVACITHVVLA
                     PAPRTRGVINVNTSETKQVVRGMSTEAANWTIELFNPLAGLPGVINALNVQDDFTRAP
                     YKPDDDLGLPGAVYSKGLSMDPMRRRWQTTEEKNFLNQLGSFPPPVQDDRDRPSLLYG
                     SDPNAAPEWNENRDGRAVLRLSSLITAGRPHHGDGRYYESIGDLLYGSYAGGIALIDG
                     NDGPWPLSNLGLPTQGNSSSFTAMNDVDQLVLRYNHQMEARHDEIVKRFRMMSNLITT
                     RSNVFEIIATVQAGQVSDLNGDGVLDYRGGEFTPLAETQGRMVYDVRPRAVFQDEYRP
                     TAP"
     gene            complement(22052..22678)
                     /locus_tag="PKN92_04760"
     CDS             complement(22052..22678)
                     /locus_tag="PKN92_04760"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNZ48136.1"
                     /translation="MNLRWIDVTLPMHRGMTLWPGDPPFSLEADVRIARGNSCNVSKL
                     SLGTHTGTHLDAPWHFIENGAQLENIDKSLFFGTALILDCSGSHQINAADLPAERLPE
                     RVLFKTANSRIAEDSSFVQDFTGIEADAAQRLVDDGVRLVGIDYLSIGPYKRGEAVHK
                     ILLGNGTIIVEGLRLANVPAGKHEFIVLPLRLLHADGAPCRAFVGVAG"
     gene            complement(22697..23878)
                     /locus_tag="PKN92_04765"
     CDS             complement(22697..23878)
                     /locus_tag="PKN92_04765"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNZ48137.1"
                     /translation="MFTFFFTTLFLSASFQAEYIFPPNEMHNHSSSIVECSDGSLLAA
                     WFHGTGEREADNVVIQGARKKAGDNHWSEVFLMADSPDLPDCNPVLFIDPRGVLWLFW
                     SAIQDNEWGSALLKYRTSRDYLGDGAPKWDWQDIIHMRPLDLEERFFPVVDEGLNTYS
                     SLIETLAPEKKDQIAAIRKQGEEKLTQRLGWMGRTTPIMLDDTTIMLGLYSDVFNYSL
                     AVFTSDWGQHWKSSAPILDDDLLMLGNIQPAFVKRSNGDIVAFMRDNGLPQQIRQSLS
                     TDQGMHWSKVDFIGIPNPGSSVSALALQSGVWLLVCNDTTLGRHLLTVYLSDDEGKSW
                     HVSRRIEEAPEGEGNFSYPTLIQGADQTLHLSYSYRRSGVEGSTIKHAAFTENWVRGI
                     D"
     gene            complement(23993..25888)
                     /locus_tag="PKN92_04770"
     CDS             complement(23993..25888)
                     /locus_tag="PKN92_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ48138.1"
                     /translation="MSFPVDVSAYKAVAINPFTDEITSEQRAQLLTNIQIMRDTIIFF
                     TAVAGAKGLGGHTGGAYSIVPEALIADAFMKGSDRFYPVHFDEGGHRVALQYALSAFN
                     GEMPFEKLLQYRAAYEGLYGHPELDPELGIKFASGRLGHLWPFVNGVACANPDKVVFL
                     LGSDGSQMEGDDAEAARFAVSRQLNAKLLIDDNDVTISGHPSKYLPGFDVAATLKGHG
                     LATCVGEGEDFDSLYQRMVTAVRQEGPVALINKRLMAPGVPVVEGTSAGHDVIALAPA
                     LEYLKARNLTDAIAYLEGVKKVSGNPVYKGCTAETASNRGEFGKIVNAILDRMPEAER
                     IDKVLVVDSDLEGSTGLKVIRIAHPEVCTNGGVQERGNFSAAAGFGFKEGRQGIFSTF
                     SAFLEMIISELTMARLNDANVLCHFSHAGIDDMADNTCHYGINIFLADSAIAEGDTTR
                     LYFAADALQLKSLVETIWNDKGARFVFTLRSATPFICKEDGSRFYEAPYQFVPGKDEI
                     IRTGTQGYIVSYGDMLSRSLDAVEQAREAGLDVGLINKPTLNVPDEKTLALLSKAPFV
                     LVVESQNAKTGLGSRFGTWLLERGFTPRYAALGVTKHGKGGLSEHMPHQGLDPASILA
                     KIQSLSV"
BASE COUNT         6333 a         6389 c         6799 g         6381 t
ORIGIN      
        1 atgaattcaa gaatttcggt gcgctgtgcc cctctataca aagatgtgtc ccggacatcg
       61 tcgccctgat gataccagac aacatcaaat tcactcagtt ctcgcgcagt gcctgaacgg
      121 tcaataaaac ctgcattgtc gcggttaaaa agaatttccg tatccggctg ttcagccaga
      181 aattgccagg cggcaaggct gtgctcaccc atttcttcca gagtagcttc ggctgtcaga
      241 aaagcaacac gcattcccgg gcgcagtgtg ttgtcttctg ccgcactgct gaatggaagg
      301 ctgaaacaaa aacagatcag aaaggataaa ggaagaaagc gcatcataat gtagttcctg
      361 ttagctgaga gcaaagtgga caacgttaat actgacgtat tgaaatgaat agagtcaagt
      421 aattacactt aaagcgttct tttgcaaatt aaagatctct ctgatcaata agtcattcgc
      481 tgtttacggg tcatcacagt aaggatatac catggaactg ctgaaagaaa tctgggcatt
      541 tatgcgtgtt cgcaaaaaat tctggcttgc cccgatcatt atttccctta ttctgctcgg
      601 actactgttt ttctttgccg gtcaggcggg cgtggtttcc ccttttattt acgcgcttta
      661 agaaaaacgg cccttatttt cgcagattcc gaccggatac ctgtccgccg gcgcaggaga
      721 atacccgaat gcctgagagc catttacccg tagaatcgcc gtccgatgcg ggacataagc
      781 ctttgctttc ccgtacagaa ttatgggttc tgacaggcat tttaattgcc gctgccctgc
      841 tgcgtttctg gtatctgtcc gctgtgatgc atgcccccga ttactcagca ctgcagcagg
      901 atcctgaagt gcaggatcat tttgcccgtg ctattctgac gggtgactgg tcggagcgtc
      961 ccggagagac cgatccggaa atacggacga cgccttttta cagacctccc ggctacggat
     1021 atcttctgac agttctctat tttttcagta aaggaagtta tctggcgccc cgcctgctga
     1081 atgtatttct cgggcttgcc gctgtttatt ttctatttcg tctcggcagg catcttttcg
     1141 ggctggtagc cggacttgcc tgtgctttgt acagcgcttt ctgcgggatt tttatctatt
     1201 gggaaggaga ggtaaacgat cctgccattt tcgtgttcct tgctgttgcc ttattctcac
     1261 ttcttcttcg atggagtgcg tcgttcaaac tccgctacct gttcctgctc ggtcttttgt
     1321 tcggcgcata cgggctagca cggccgaaca tcctgggctt tggacctttt gtcgctttat
     1381 ggctcggctg gattgcgtgg cgacataaaa agctgcgcct tcttccggcg gcatggggta
     1441 ccctgctgat cagtactttc attctcatcg ttccggttac agttcgtaat tatgtcgcat
     1501 ccggggaatt tgtgcccatt gccacttatt tcgggcacaa cctgattatc ggtaatcatg
     1561 agcagtcgga cggtgtttcc ccgtggctgc cttacttgca ggaactggaa ggtacgggaa
     1621 aatgggcggc aaaagactat gtgaatgtag tacgcgggct cgctaaagaa ttggggcgcg
     1681 attccatctc tcacacggaa gcctccgctg tttttgcgca gaaagcacgg gcgtatatgc
     1741 gtgcacatcc ggcggaaaca atcagatcta tggtaaaaaa atggattctc ctttggacac
     1801 cagtggagat tacctgcaac aaagtggtgc aatgcgagct gaatcattat ctgccgcttc
     1861 gctttctgcc gactttccgc tggatagcgg cactttttac ctccggtatg ctgctgctgc
     1921 tctgggcatt gttcaggcgc acttttaacg gcaggcatca ggatgccatg cctcttgtcg
     1981 ttttaatgat actttttgtt ctctattacc tcacgtcttt tcttcccttt ttcgtcaacg
     2041 gcagggcgcg ggtacccgtg tttcccgttc tgattctcct cgggagctat ggcgttcaat
     2101 gttgtgctgg ctggctaaaa aacaggcagt atgggaaagt tgccgcatac ggaatcactt
     2161 ttctgttgag cttgatgttt ttttcagttg aatggattcc tgttgaaatc gatgcatccc
     2221 gctggcatta ccagcgcgct gattcctggc tgcggcgggg cgaaacggca aaggcgttgg
     2281 aggaaggaaa agctgttatt actgctcagg gcaaagccta tatgcatcag cggctcggca
     2341 gagaattcat ggcactgggc tatccgaatg acgccatcgc acagtaccag gctggactcg
     2401 agcgggaccc cgaatatcag gatttatggt ttttactggg gcgtgctttg gaagaacgag
     2461 gtgatttgaa agaggcggaa aagacgtatc gggacgcttt gactttgaat ccttttgatg
     2521 cccgggcgct gaatgctctg ggcatgctct gttacggtca gaaacgctac gaagaggctc
     2581 tgaaactttt cgacgctgca ttaaacgtgg ctccggatta cggggacgtt attgaccaca
     2641 tcagccagtg tatgaaggca ctggggcgtg ttgatgatgg catcgcccgg tttaaatcaa
     2701 tgcttgcaga aaatcccgac cagaaatatg tgcatttctt tctgggccgc gatttactgg
     2761 agctgggaca atccgaagag gcctttactc attttgccga agcactccgc ctggatcctg
     2821 cccttgacgg agccagttat ctgtgggcaa aagaactcag ccgacaggga aaactggagg
     2881 aggcgatcag actgcacgag gcgacgctga agataaatcc tgatttgaaa tatacccagg
     2941 cggcgcttgg aagcgacctg tccaggctgg ggcgtacgga cgatgccatc cggcattttt
     3001 cagaagctgt gcgtattgat ccttcttttc aggatgcgcg ttacttgctt ggaaaagagc
     3061 tcgcccgtgc caaccggctc gatgaagcac aggcgcagta tcaggaagcc attcgtttaa
     3121 acccgcagga tgcccgggca catcacgaaa tgggcatgtt cttcgcagag cagggtgatt
     3181 ttcctgaagc acttcagcac tttgaggcgg ctttgaaagt ggatcccgaa ttcgcttttg
     3241 cgttggatcg catgggccgt gttctttcga ttatggggcg ttcagacgaa gccatcgcac
     3301 gctataagca ggctctggaa gtaaagcccg ataatcagga tctctgctat ttactcggtc
     3361 gtgaacttct ggcgcaggac aaaaaagacg aggcggagcg ctatttccgt cgggcactgg
     3421 tgcttaatcc atcggatgcc agggcacaca atgaactggg cgcactcctt gccgctcagg
     3481 gcaaaaaaga tgaagcgatg cgtcactacg aagaggcact gagagcggct ccgcagtttg
     3541 gttatgtgct ttacaaaatg gggcagctgc tcgctgagga aggaaatctg aaagccgcgg
     3601 aggcacgctt taaggaagcc ttgaatctgg atccggataa tcaggatctc tactatctta
     3661 tcggcagaga gctggtatct caggaacgta atgacgaagc gcttgcgtat tttgagaaag
     3721 cgcttgccat gaatccttcc gatgcacggg cgcataatga ctatgcgttg cttattggaa
     3781 gtaagcagcc cgaggatgcc attgcccact atgaggcagc tttaaaaatt gcacccgact
     3841 tcaccctggc gttgaataat tggggtaatt tgctcctgga actgaatcag ccggatgctg
     3901 cagaagaaaa attccggaga gcgctgcaac acagacccga tgacgaatac gcctggtaca
     3961 atctggcgcg cgttgccgaa aagaaaggcg atctgcaggc tgcggaagaa gcttacagga
     4021 aggcaatgga aaatagcccg gaaaactcac aaattcccaa taacctcggg ctgctgatgg
     4081 cgcgtcaggg aagagttgtc gaagcgcaag aatattacga aaaagccctt tccattgatg
     4141 atgaaaaccc actcccctac aacaatctcg ggcaattggc ggaaaaatcg ggcgatgcgg
     4201 aaacggcgat aatgcgatat cagcaggcgc tcgaaaagaa accttccttc cttttagcgt
     4261 tgaataacct ttccgccttg ctcacccgat gcgcaaaaac tgaggacatg gaagtttggt
     4321 acacccgggc gtttgaggcg gatccggata acggagacgt tcataacagt tacgcctttt
     4381 atctcttccg tctgggcaga gacgaggaag cacgtcggga gtacatgaaa gccatagcgt
     4441 gcattcctgt ttttcctctt gccttaaaca acctcggcaa tctcgaattt cgagcacaaa
     4501 ataatgctga agcggaaaaa tggtataaaa aggctcttga ggttcacccc ctggacttgc
     4561 atgccgactt taatcttggg cgtttgttcc tggcagaaaa ccggaaagaa gaggcactgc
     4621 acatgcttga atctgcggcg gtgaagcaat ctgatgagcc gcggcttctg ctggaaacgg
     4681 cgaatgcctt tgttgccgcc ggttcgctta aagatgcgat acactgttac aaacgggtga
     4741 tagaactggc gcccgacaac aaagccgccc tttttaacct ggcatcggtt tatctggacg
     4801 gatacagaga tgctgcgcag gcgcgccctc ttctggaaaa cctgcagcag atggatcctg
     4861 attttccggg gctggatcag cttctggcaa agatgaagca atagcagaat cggcaagcag
     4921 cggattattc agcgctgctt tccccactct gccgactttc cagaggtgct gcaaaaacag
     4981 caagattgtt tccgtttttc cgttccacgc cgggcgggtt cagcacgata ccttcaggga
     5041 cagttgcagg cagaggaaca ttgacaactc gcggctccgg atcggcgagt acaagtgtgg
     5101 atgaaccacc tccatccaga gcgagcgcac tcacggcgtt aaattccaca aagagtgttg
     5161 ccatgtcatg atatgtcagt ccggtacaat ggccgggctg tcttccatct gcgataagga
     5221 acagcatttt ctgatctgct gtaagcccca cagcagtacg cgggtggcgg tcaccttcct
     5281 cttctgtttt gacctttcca tcttccacga gcatgatatt gcctgtcaag gcattaaaca
     5341 gtttgaccgg cggtactgtt tcactggtat tttcgccccc ttccgggcgt gctacaaaag
     5401 tgacctggtt gtcttccgca atattgacgg catattttgc ccagccttta tcccacagtg
     5461 aaacgacggt tccatctgat acagcaagtc ccagcagatc cgtgtcttcc tgatgatcat
     5521 tgataaaaaa attgccgtta atgcccagtt gcgctttttt ttctttgaca aaggcaagtg
     5581 ttgtttcgca gcgtgtttcc cgcctggctt tttctgtgtt gggcggtgtg gaagtaaaac
     5641 ggatcgcagg atcctttaaa tcaattttaa ccagatgaat tcgctgcggg agaggagtct
     5701 cctgatggat aaatagataa tcgaccccgc gaaacacagg gatccaggcg tggcattttt
     5761 cgggactctc cgcaaaagca gttgccgtaa agaaaagaaa gagaaagaac aatgccggaa
     5821 gagacaggct gcctcttttc cggatctttc ggtagaaagt attggttgcc gacatagaag
     5881 ccaaagtgaa ttcctttaca gtgccgggct tttcgttgcg gttctttttt attttggtga
     5941 ctctccagta taattacaaa aaaagaggcg atgaatccat taattacttt aacagaagga
     6001 agatccatgg ctgatagtat caaagcgagt aatataacct ggcatcatgc aacggtgtcg
     6061 cccgaagacc ggtcggtgaa aaacggacat cagggggcca ttctgtggtt taccgggttg
     6121 tcagcatcag gaaaatcgac ggtagcgcag gcagtccagt ccgtgctttt tgaaagtggg
     6181 tgtcaggcct atgttctgga tggcgacaat gtccgtcacg gtctgaacaa agacctcgga
     6241 ttctcaccgg aagaccggga agaaaatatt cggcgtattg gcgaagtgtc tgcgcttttc
     6301 tgtgatgcgg gctttattgt gagcacggct tttatctccc cttaccgcgc tgaccgggat
     6361 aaagctcggg ctctttgccc ggatcgtttt tatgaaattt ttgtgaaatg cccggtggaa
     6421 gtctgtgaaa cacgggatcc caaagggctg tatcaaaagg cgcgggaagg gaaaatcccg
     6481 gaatttaccg gcatcagtgc cccctatgaa gaaccagaaa aaccggagct gattctggat
     6541 acagcgacac tgtctctgga agcctgtgtt gcggaagtaa tccagatgct gcaaaaagag
     6601 ggcatccttc aggcataaca gccaatttct acagctgggg cggacaggtg tcccccgctg
     6661 agggcgaaag tgcaggattt cggatgatgg aaatcctgca cataagtgcg taggttgcga
     6721 gacggatcag gcagagcagc aacagcatga cccgaaggcc cccacccccg tacaagtggt
     6781 agagcacaaa aagtgatgcc atggcaataa ggcgccccac agccagagga ctctcccagg
     6841 cgcacaggta ggcaatgcgg tcgccaggct ctttcatcat gctttccatt acttcaaaac
     6901 gaacagcagt gtccggtatc ccgtacaagg gcagcgaaat ggaacgcaga aaaccgaaga
     6961 tcaggagcgt ccatacggtt gtttcccaga agacgatcaa acctgccgcc acaagcacca
     7021 gcacccccca gaacataaaa cgtttgcgcg actgaggcac cacatagcgg cctacaagat
     7081 aggacaccgt aacagtgacc agcccctgaa atgacacata gcctcctacg gcggcttcat
     7141 ttccgatttc aaaatacatg atcagcgcca gcagaaaatg aaaaatatga aaagagcccg
     7201 ccagtgaaat gcctgccagc atgagaatgc gccagtcccg atgcgctctc ggaggaaata
     7261 aagcctgctt taggggaaaa gagccgcttt tcgggcttgc agggatttta aagctgaaag
     7321 tgatggcaac agaataaagc atcagtgcca caacaaaaat aatttcgtag ccctgctgcg
     7381 gaacaggtgc aaaagaaata atgagtccgc tcatcagtgg cgccaatatt ttcgcaccgg
     7441 cggaaacggc agaaatcatg cccagaaaga attctcttcc gccatttcct gagctgaaat
     7501 catactgata tgtgttgttg cctgcccaga aaaaacccca cgtgatccca agcaaaccgc
     7561 caagcattcc ggcatattca ggggagctgt cccgcagaaa cagcaatgcc gcataataca
     7621 gtgcatgcaa aaccaatccc gccctgaaca aaaaactccg gtctctttta cgggtaagcc
     7681 agccggcaaa aacaaaggca aaaggagttg tcaggtatag cacagtataa tgaaaaaaaa
     7741 ctgtattgat gttgtggctg ttgatataga aatagacccc tacaaagacc gagcaaagcg
     7801 cttccgcaat gctgtacaaa gcattgaggg caatgatcag ccgggcgtga ccggagagtt
     7861 tttcctgtcg cctgaaaatc cagggcacta tttactacct ttaaggcttt gcttgttata
     7921 atgaaaacgt ctgaagtgta agggcatgcc tgaccggttt tccggatagg gcgtatttct
     7981 ataataacaa aatggaagat gtgatgacag aaagcgctta aagcagcgcg acaccatggt
     8041 ggaacctaca atggcagaat atgttcttga aatgcttgat atcaccaagc gttatccggg
     8101 agtgatggca ttaaataaag cgcagcttca attgcggccc ggagaggtac attgtcttgt
     8161 cggagaaaat ggagcaggga aatcgacttt gatgaaagtg cttgccggtg cgattgcccg
     8221 ggatgaagga agcatccgga tcgacggctg cgagtatgac taccatactc cgttgcaggc
     8281 ggcaaaactg ggtatcagca tgattcatca ggagttcaat ctggcgccgc agttaagtgt
     8341 agctgagaat atttatctcg gtcgtgctcc ttccagccgt ggtattattc gctggcgaca
     8401 gatgtacgat atggcgcgcg atgcgttggc aatgttggat tctgatctag atcccaggcg
     8461 gctggtcgcc acgctcagtg tagctcagca gcagatggtg gaaattgcca aagccctttc
     8521 gattaatgcg cgcattctcg tcatggacga accttccgcc acgcttactg accacgaact
     8581 ggcatccctt tttcacatta tgaaaattct gcacaggcag ggcatgggta ttatttatat
     8641 ttctcaccgg ctcgaggaga tttttgcagt cggcaaccgt gtaaccgtca tgcgagacgg
     8701 ctgctggatt ggtacgcatg atctgtgtga cgtgacccgt gaggaattga tccgcatgat
     8761 ggtgggcaga gaactgcagg acgaatttcc gcatcgaaat gctgaaatcg gcgaggtcag
     8821 gctggaagtg cagtctctgg cacgccaggg atccttttat cccctgtctt tttctgtgcg
     8881 tgcaggtgaa gtcgtggggc ttacgggtct tgtcggttcg ggcagaacag agattgcacg
     8941 cgccatattc ggggctgatc cgcccacgtc cggcgctgtg ctggtggatg gctgtgatgc
     9001 aacctccaga accccccgcc aggctatcag gaacgggata ggactgctga cagaggatcg
     9061 caaaggccag gggcttgttc tgggcatgag tattaaaaac aatatttcgc tggcaaatat
     9121 gaaggcggtg cagaaaggcg tttttctgtc gccatctctt gaaaaagccg ttgctgaatc
     9181 ctatgttgct gaactccaga taaaaacgcc gcacgtggaa gaaatcgctc accatctgag
     9241 tggcggcaca cagcagaaag ttgtacttgc caaatggctc ctcaccgatt ccaaagtact
     9301 gatttttgat gagccgaccc gaggggtcga tgtaggcgcc aaagttgaga tctatcgttt
     9361 gatcaacacc atcgcatcgc agggcgttgc cgtgctgatt ataacaagtg aacttccgga
     9421 agcgcttggc atgtgcgacc ggcttctggt catgcgcgag ggacacctca tgggctccct
     9481 ttctattgaa gaggccgatc aggagaaaat tatggagctg gcaaccggtg atatccgcaa
     9541 agccgtgcct gtatgagacc gtatctttaa aaacctgtct ttgaaaagac cacgacctta
     9601 aatttgcaca atttaccttt tgtgtaagaa tgttttttag tcagaaaaac ggaggagaat
     9661 ctgatatgag caagcaattg ctgcttttgt gtcttttttc cctgatgatt atgggcagca
     9721 ttcaggcaga attatcagcg cctttcgcgc ttaagtgtga gtatcgggtt aatcccgagg
     9781 ggattgatac gtttgctccg cggttctcct ggaagctgga ttcgcaggat cgcgcccagg
     9841 cgcagagtgc ctggcgaata cttgttgcga gttctgaaga aaaacttgcg aataatgagg
     9901 gcgatctttg ggactccggc attgtgcgct ctgataatgc cattcaagtg atgtacggcg
     9961 gtgtgccgct ccaatccttt caaaaatgct ggtggaaagt gtgcgtccag gaccaggacg
    10021 gtaaggaagg agaatggagc gaaagcgcat tctggtctat gggtgttctc ggcgagcagg
    10081 actgggcagg tgctaaatgg atgggcatgc ccaaaggcga ggaagaatca gccgagcagc
    10141 ttgcattgaa cgattctttt tggatctggc attcagaagg aagcccgcaa attgaagcac
    10201 ccatcggttc atgttttttc agagcccatt ttaccctgcc caatgaaaag attaatcagg
    10261 cggcgctgat gctttccgtc gacaattctg ccgttatctt tgtgaatgga gaaaagatag
    10321 gcaaatggac cacttatcat agtgcaggaa cttttcccat tcagaacgct cttgttcccg
    10381 gcattaatac tctatgtatt gaagccgaaa acgctggcgg taatcccaat cctgccggaa
    10441 tgattgccct gattcagata gaaaccgcat cgggcactat attgaaccga agtaccgatg
    10501 cttcctggaa aagctcttat aatcgtgaac cgggctggga aaagagttcc tttgatgaca
    10561 gtcactggaa gacagcgcag gttctcggta aaaatggctg tgaaccctgg ccgattgctt
    10621 tgattgctga agaccgtacc cttcccgcca ggatgatgcg gcgggaattt gaggtggaat
    10681 ctcccttgaa acgggccgtt gtttcctttt ccggattggg gctttccgaa ctctacttga
    10741 acggagaaaa agtcggagac catgttctct cccccggtct cactcagtat aacaaacgaa
    10801 tattctatgt cacccacgat gtaaccggcg atctccggga agggaaaaat gccttgggct
    10861 tatggctcgg taacggtcgc tattttgccc cccgtatttc cgacccgaca aaaacagaaa
    10921 ccttcgggct tcctgaagcg cgcctggtgc tgcgcctgga atacgaaaac ggaagcgtgg
    10981 caaccgttgt tactgatgaa aactggcggg tgacggatca gggacccatc aggaaaaata
    11041 acgagtatga tggtgagact tatgacgccc gcatggagct gccgggctgg gatatgcccg
    11101 gttttgatga cagcagctgg cagcaggcgc aggaacttga aggcgagcgc ggcgcccttt
    11161 ccgcccagat gatggagccc atccgggtgg tgcgttatgt atatccgaaa gcaatagcca
    11221 atcctgcccc gggaaaatat atttatgaca tgggacaaaa tatggtgggc tgggttcgca
    11281 tgaaagttga gggcccgcaa ggtgccgtgg tgaaacaacg ctttgcagaa gttgtgaagg
    11341 acgacggaac actctacctt gataatatcc gtggtgccaa agtcaccaac gaatattacc
    11401 tgaaaggcaa gggtgaagaa gtgtgggagc ctcgttttac atatttcggt ttccgctacg
    11461 ttgaaattac aggctatccc ggtgttcccg ctctggatga catcgtcggc tgtgttgttc
    11521 atgatgatat ggaaacagca gggcatttca gatgttccaa tgacctcgta aaccggattg
    11581 tgagcaatat ttcctggggc gtccggggca attaccgcag ttttccgaca gactgccccc
    11641 agcgggatga acgtcaggca tggctcggag accgatctgc tgagtcacaa ggagaaacct
    11701 atctgttcga tgtggcggcc ctttatgcga aatgggtttg cgacatggaa gacgcacagc
    11761 gggaaacagg gagcgtgtcc gatgtcagcc ctgcttattg gcctttatac aacgacggcg
    11821 tgacctggcc gagcagtttt ataattattc cgggtatgct ctaccggcag tatggagatg
    11881 catccgttat ggcccgtcat tatgacggca tgaaaaaatg gatcacccat atgtccggct
    11941 atataaaaga cggtattatg ccgcgtgata cctacggtga ctggtgtgta ccgccggaag
    12001 agcagcatct gatacacagt caggatccgg cgcgcaaaac aagcggagat tttatcggaa
    12061 cggcttactt tatccatgat ctgcgctgca tggagcagta tgcgtcactg ctcggcaaag
    12121 agcaggattg cacgcagttt aaagcacagg cagatcggat gactgaggcg ttcaaccgga
    12181 ctttttatag cagcgatcag ggtttttatg tcaacggcac ggaaacttcc tgtgtacttc
    12241 ctctggcatt cggattggtg cccgaagctg cccgctcctc ccttttttca catcttgtaa
    12301 ccagtataat ggataaggga caggggcatc tctccacagg actgataggc ggtcaatggc
    12361 tgatgcgcgc tttaacgcaa ggcggacgcc cggatgttgc ctggacgctt gcaagccaaa
    12421 gcacctatcc cagctgggga tatatggtca agaaagatgc aaccaccatt tgggaacttt
    12481 ggaacggcga cacggcggat ccggcgatga attcgcacaa tcatgtaatg ctggtgggtg
    12541 atttaaacat ctggctctat gagaatgtgg caggaatccg cgccgcagcc ccgggcttca
    12601 aaaaaataat tatggatccc gttatgccgg aaggtctgac ttttgccgaa gcctcttata
    12661 attcaattta cggacagatc ttcagccgct gggataaaac tgacaatgag attcgctggc
    12721 gtgtaggtat tccggcaaat acaagcgcaa aactgcttct tcccgccgga gatccctcgg
    12781 taatcattga gtccggaacc ccggcaaagg ttgcgaaagg gcttcatttc ttggagacgg
    12841 acggtcagaa atgcacgttt gatgccggct ccgggtggta tgaatttgcc atttcacagt
    12901 aataatattg ctgctgtagt ttgagaatgt gttcggttgt ctctttgcct ctggtgcgat
    12961 ctgcatcgtg aaaaatatga attgtccgac cttgcggtca attattaatt tttgcggtat
    13021 aatgtagata gtattcggtc ttttaaatta aggtatacta ttgacaaaaa tacaggcttt
    13081 gtcttttttt ccgcagaagt aatatatttt tttacataca aacggaagat caggcagtgg
    13141 taatggaatg tatgaccaga taaggtggct tatgaacatc cgaacctctc accgcgtcca
    13201 gggcttcaga ccatctgttt cacaggaacg gggagccgcg ctcattgtcg cactttctgt
    13261 ttttctcgta ttgctttcat tggcgattac tttttcgctt attgtccgat acgaaacgca
    13321 gatgacccag caggcaacca gccgtgctct ggctgaaagg cttcttgatg gcgccatggc
    13381 gcaggcccaa taccgtttaa acagggacct ggatctgcat gctgatgcaa tgagtctgga
    13441 tcatggctgg cgcacccggt ttaacggagc tgcctttgtg ggcaagagtt gggcgcagca
    13501 atttcagggg gagaaagcgc accagcccct atataaaaat gccgaaggac ttatctgtgt
    13561 taatctggaa ccggtcgaaa gagtgctgcg cggtatggga cgccttgcgg atggtgtcct
    13621 ctatgcacac tttttaaaag atggccatct ggaacctctt tttcgtggtc cccgtacgga
    13681 atcctggctt tacgtgcccc gaatgcaagg gaatgctatc cttctgtatg caagccggga
    13741 tgaagtggaa ctggtaaatt cgcttgggga ggtgctttcc cctgcagatc tgaataatgc
    13801 tttaaccggg cttcgctccg aacgctttgc tttttgggat gaccagattg atccggataa
    13861 accgaacgat gaaatttttc cttttgcagt tcccaacgat ttgtcgccgg atatcgatcc
    13921 ggcaaccggg cttgttttat cggcgcttcc ccaggaaaaa gtgaatcagt gggctgatgt
    13981 ggacagcacc ggtgacggaa tgagcgatgc catttggatg ccgattgtgc aggatgttaa
    14041 ccatttcggg gatggcatcg acaatatgtt gagcggaaga ccggatcctg cccgcagtgt
    14101 tgacggtgag tttatcccgc ttcatgaatc ggctcctttt gtgtatcacg gtttagggct
    14161 gccgcgtgct gcaagatcgg aggatctctt cgaacttgat cagatgaaaa atcttcattt
    14221 tgcagggcgc tttgaagacc ctcctctggg tgatggctat gacaacaata atgacggcat
    14281 ggtggatgat ctgtcggaaa atcagctgtt tttaacggtt ccgctgccgg gactcatgat
    14341 gcaggttgac ttgaacagcg acggtctgtt cgatgaacgg gactactacg agttcaataa
    14401 ttatcggggt ccgctttatg tgcagctgcc tccaattatc tatgtgccgg tcattgtggg
    14461 catggatgcg ctgggcgagc cactttatga tcgtataccc ttgagcattg atgctgttga
    14521 tatgctcgat aatgattatg accttatggc gaataatttc agaagttacg cgtacgtagg
    14581 tccccatgag aaattaactg atgcaagcgc tgctgatgtt tatgccggaa aacaaatccc
    14641 cttttcccgt gcgagtgcag ccgaggcaca tgcgcattat acggcatgga tgccttataa
    14701 agaccctgta gaggatacag taggctggct tggtcccgac aggaaggata gacttttttc
    14761 tgacgcacat caccgcgctt accatgacat taatgtcgat gccgttctga acggtcccgg
    14821 cgcacttcgg ctttttatcg ttcccgccga tgccgcttca ggcggtggtg tgctttcaaa
    14881 agtggagctt cccgcaggaa atcctctggc aaaaattctc cgccagccca atgcgctgcg
    14941 tgtgacctgt tccggcgagc ctgtttccga agtcatcggg cgctatgctg ttcatatatc
    15001 agatgaatca ggcaaagtgg atctaaatgc cgctggcggc tactactctg atgaaattag
    15061 cggagatttg ccttctttgc atctggggca gaccctcttt gaatataaca cgagagtgct
    15121 tcccgatatt ggcaaggggc gcagctacga cttctggctg atgcgcaccg gcagcgatga
    15181 ctcgtggggc acggtagatg aattctttcc gggctaccat ctggtggatg ataatggaaa
    15241 cgcgctgctg cttgctctga aaggctggaa ttacagcgga cactttttaa acgggaaagg
    15301 gctggatatt cccgccatgt ccggagccac cagggagtat ttgttcaaca atgtgtcgtc
    15361 gcctcttgca acctatgaag atcaaacgct gcgcttgaaa caacgactga cgatgctggg
    15421 gcgttttgac ggtatagacg aaccttctga attacagttg tataacccgc ttaaaaatcg
    15481 ggaagatcct gccagaagtg accgcctctt cttgcggaaa gaggacctga aacgccatgg
    15541 ggacgtgggg cccaaccgct gggattatct gaaaaatctg gtgacagtca acagtgaaaa
    15601 gcggaataca ttccacacaa gaaattccgg taagacagaa aacttctacc agattgatcc
    15661 taacacggca acgccgcagc agatcgcaac cgccatgttg ttccgggctg atgtcactaa
    15721 cgtggtggat aagatgcacc tgccgtcttt tgcagaaggg cttcgtcagt tcgatacagc
    15781 cttcagcggt gatttaatgg gcaaagtcaa aacaggactt ggaatgggca ccaccccatt
    15841 cggaactttc ttccctgccg atcccatact gcagattatg cgacttgctg ccgatataac
    15901 ttccgccaga aacccgaatc ctggtgttgt aaaactgact accgatcctc tgccgcgtga
    15961 ccccactatt tatagaaaat ataatctttt ctccggaatt gctaattcca ttctactgcc
    16021 tgctgaaagt tggaatgaaa gagaacggat tgccgacgaa aatgtgctgc cgacaggtga
    16081 tttgcaggcg gcgctgacag gtacttcggg agaagagttt gatcaaaaac gcctgatctc
    16141 tcctgatacc tggtggaaac agcagacagg ggaagatcgt ctgattcaat atgccacatc
    16201 gtctttggat tccatccgaa ttaatgaaat tatggttcgg cccgtgcgcc gtgtcgaagc
    16261 ggaaatggta gcaggcgctg cggttttgcc tgagttaaat cttaatccgg cacctgttga
    16321 cgggatgctg cctttcgagc ttgagcgacg gttcctgcag aatcctccgg agaattggtc
    16381 tttacgttcc gcctatgatt atcccggtca cgctcaaccg ttgctgggtg atcggaccgc
    16441 ctgggtctat actcccgatg acccgcttgt gcctgctgat ccccgggacg tcatggagtt
    16501 cagatttaaa gaatcgcctg gtctgccagc aggcaactat tatctgctgg caaacctgac
    16561 cgatcaatat ggaaggatga ctgttgccga tgtggacgtg ctggaatatg ccatccgcta
    16621 taccggcacg ggcggtcccg gtgtttttga agatttcgct gaagaagcga gccttgccgc
    16681 actttttgcc cagatcggct atgcaggtac agccagcgat cttcagctac tgacatccaa
    16741 gaactttcaa cgtattaatg cgccggaatt tctggctacg gaaagcatgc gcatgcatgg
    16801 ttccgcctcc gctccaggct gggctttcct tccccatagg aatgcttttt tagaactttc
    16861 agatacaccc aatctgcttg atgactttga gtattacatc aaaacgaaac ttcccggttt
    16921 tgccctgccg cctgatgcga ccatgcagga tcttctgctt actttctgga cggtggttat
    16981 gggctgccca tctcccccgg ctgatgccac ctgggaggca ctgaacgaat acgccactgt
    17041 taatggctgt gtgctgcctc ctcctgccga tcccaatgac ttgctggatc cgttaacaac
    17101 ggataatgcc tggtataatc tcctcgggtt gctctcccgt ttttacggga taggaggtgc
    17161 ttattcagca ggcggcacgg aaaccggcta tacagtattt ataccgcctc tggcatcagg
    17221 ccaggaatta gttgttgcca ttcgcatgaa cccggtggta cgtgcttctt atcaggaagt
    17281 cattaacgag tgcattgcaa acaacgactt tgccggcatt ccggatcctg ttactgtggc
    17341 agattggcag tttctgcagg ggctcatttc agcgggtgtt gttcttcgtg atgcgaacga
    17401 ctttccgaag ccgctatttg ccattaactt ctttgatttc agccaggaac caagtcatga
    17461 gtatattgag cttgcgaata tctccgatga agaagttgat ctgagcggct gggttcttga
    17521 aataggagtc cccgatcccc ccggtgcaac ccatgatccc atgatgaaag atccttataa
    17581 gagccagtgg gaaatccctg ccaatattaa aatagcgcca caaggctacc ttcttctgag
    17641 tgccgatatt gccgacagta caggaaacaa ccacctgttc aattccaaca ggttgggctt
    17701 gaagtcggac ggcacggacg caatcactgt accgcctatt cacaaccagt tctctccgga
    17761 tcctgtggcg cttcccgtgc tggagcctct cttgcatgac gaaacgggaa gtgtttttct
    17821 gcggaaagac ggcatcgatt acattgataa tgatggagac ggtctgtcca gtcagcttat
    17881 gtggggtgta aatgctgtac cgacaactga aagtgatttt gaaaggcggg ataccgatca
    17941 tgtgctttct gaaatgagaa cccatggaac tggacccgga ggtcagggta ttcccgccgg
    18001 aagccgtatt gtgccgctca taaaccgcca gctctggaag gaaaacagcc gcagccatcc
    18061 cgatgcaaat ccgaccgcta tggagaagta tctctataaa ctgaatcctt ttacgcccga
    18121 tcagcaatcc gtaaatatca ctgatctgaa agacacggag gaaggactca aatatttgag
    18181 cagattcctg cttcggggcg gtctgttccc caactatccg gaacgggacg gtatagacaa
    18241 tgacggagac ggcggctatg cagtgcgtaa ctccataaaa ggctacgtgc ctgtcattcc
    18301 ttccgtgtat gagcaggaac tgatcagtta tgtgccgggc acacttgata aagatatgat
    18361 cgataacaat ctgaatggca tgatggataa ccgtggttac gagcgcatac gggcgagtca
    18421 gaattatact cccagcatta acctccctgg ctttacctat gacagccatg ccaacccgct
    18481 gcttagcgaa ggggttgatg aagggagact cggctttcca atctggtaca ctggtggcat
    18541 tctttcagga tttcagggtt tcctgcctcc tgcagcaaca ggactcccgc gcttttacgg
    18601 tcctggctcc tatgagcaga ccttcctgcc tctttataat ctgaatacac cagccgctgc
    18661 tcaagactat aatgagtggg cacgacggat ggcagccggt acaatttctt tttcaatcga
    18721 agatccgtca ctcggttaca ctatgactgt ggcgccggca agctttaata caggttttgc
    18781 acctgttctt gccgcttccg attctcccga ctggcaggct tttgtcgaaa ggcgctggaa
    18841 ctcaggagac aatgtgatcg tcactcttta tgtggggcat ccttccagat ccattatcgc
    18901 tgatcaggtc acctacactg agcaggatgt gatcaacagg acgaaagacg atattgtcga
    18961 ctgtccctac accattcagg gatttacaag ttacggtttt gacggcagcg gaaatctagc
    19021 gtgggtgccc ccttcctcag cctcggcacc ctacgaaccg gtatgcctcg cgcctgctga
    19081 tcccggaagg cattctttct ggctgcccga tcatatgggg cttgattttt atcgctctct
    19141 tgaacgtaaa caccccatgt atcacggaga ccgcttcggt cttgccaacc gctggacggc
    19201 aacagacggg gcatatgatg actgggcaga aagcatgagt ctttttgaaa caggactggg
    19261 gctcatgaat ctggatacga atgatgctcc gattgcggta cgctggcccg gacgacaggc
    19321 gtatatggca gaccatacga ctgcgaccgg tacttaccgt cagcgctttg atcagaaact
    19381 tgatccggcg tataaagatc atgccctgca gctctacggc catgctttct ggggatctcc
    19441 cttacgcatg aacacacagg cgcggctctg ggagaatcct ctggatattc ttcaggttaa
    19501 cagcgatgtc aaagagtggc tcaacccgga agcgtcccca atccggaacc agcggtatta
    19561 tcggcagttt gcagggctgg atgcataccg gaacacggga cacgacctga atctttctat
    19621 aaatgtaaac agtcttacgg atgatccgac aaaagacaaa gaaagacgtc tttcttctga
    19681 ctgggcggtt cgtcgggcaa aactgaggaa aaagcccttt gcaaccttgg gtgatctggt
    19741 cgaacttccc tgcttttcct tcgaaaaaat aattgaaggt ccttctcagg catcagcgga
    19801 atatgtgcct cagtacgccg tcagtgttgt caacacgcac tatatgtcta ccgaaacaaa
    19861 aagcagcggc ggtaccggag tgcttcacta tagcaatcat ctggaactct tcacctacgg
    19921 agctgatcgt gatctgacca gtgctcttct tggccaggct gtttacggcg gtggcggttc
    19981 catgcacggc caccgcggta aagataatca gccgccgacg ctggttaagg ataccctggc
    20041 tacagcagct atgaatccgg taattcttac cgttggtcag gcgcaggtgg tccccttgtg
    20101 gccggctcct gattctgtga atccgtcaac aggttccgta ttggatcccg ccgatcttta
    20161 cagtctcttc caatggaaag acgggttgcc gccccatatg tggacccctg tgtatttgtt
    20221 taatcttcct gtatacggtg actggtacga agaaaagccc tggcagacgg ttcagtatcc
    20281 gcgccggtac gacggctctt ctatcggttt ctccccacct gtcaccaatg ttctggtgga
    20341 accggtatat atttttaacg gtgaatttat atgtcgccag tatggcctgt cgctacctga
    20401 tgatgcggag tgggttgctt ccaaatggcc agtaagaaca gaggtgacct tgcaaagcga
    20461 tgcagcggga cgacgtcagc cgacagatcg tgcctgtatg tacgtgagcc gttatatacc
    20521 tgattacggt tctgaaaacc gtccggaagt gctttttgtc tgggataagg aaacgggact
    20581 tgaaaatggc gattatatgg cctatgtagg cacgtttatt ctcggaatgc gcaaaacaat
    20641 tgagcatacg aaagcggtag cagacaacta cttcggcagg actgcaacgc cgctgccgcc
    20701 ggcaagacca tcatttttat atgatggtcc caccgctgca cggaccgaag caattttgaa
    20761 ccttgatcct acgcatccgt ccatgctgaa aaatccgacg gcaccgcgct ttgatcccag
    20821 gcttgccatt gagttcataa ccaaccgcag ggatgcggag cagatgcttg aaccagtcga
    20881 attgactgcc gtttctgcgg gcagtggtca ccatggtatg ccccaccccg ctgactggtt
    20941 aagcaattct ccgggttcag cagttgccgg atactgcaag gcggatcagc agggatatgt
    21001 attctacagt gataatccgt ctttggcatg gcgcccgatt ccggtgcgtg tggaaaacaa
    21061 ctatctcgct cttcgcgtcc gcaatctcgg tgatccgaat caggttgcct gtattaccca
    21121 cgtagtgctc gctccggcac cgcggacccg gggcgtgatc aatgtcaaca cttccgaaac
    21181 aaagcaggtg gtgcgcggga tgtcgacaga ggccgcaaac tggactattg aactctttaa
    21241 tcctctcgca ggactgccgg gtgtgattaa tgccctgaac gtgcaggatg attttacacg
    21301 tgccccttat aaacctgatg atgatctggg tcttccgggt gctgtctatt caaaaggtct
    21361 ctccatggat cccatgcgcc gacgctggca aaccacggaa gaaaaaaact tcttgaacca
    21421 gctgggctct ttcccgcctc ctgttcagga cgatcgggat cgcccgtcac tgctgtatgg
    21481 gtcagaccca aatgctgcgc ctgagtggaa cgaaaacagg gatgggcgcg ctgttctgcg
    21541 gttatcctct ttaatcaccg caggcagacc ccatcacgga gacggccgtt actatgaaag
    21601 catcggtgat ttgctttatg ggtcctatgc gggcgggatt gccctgatcg atgggaatga
    21661 cggtccctgg ccgctgagca atctcgggct ccccacgcaa ggcaacagca gttctttcac
    21721 ggcaatgaat gatgttgatc agctggtact gcgttataac catcagatgg aagcgcgtca
    21781 tgatgaaatt gtgaagcgct tcagaatgat gtccaatttg attacaaccc gatccaatgt
    21841 ctttgaaatt atagcaacag tgcaggcggg tcaggtcagc gatctgaacg gcgatggcgt
    21901 gctggactat cggggcggcg aatttacgcc ccttgccgaa acgcagggtc gcatggttta
    21961 tgatgtacgg ccacgcgctg tatttcagga tgagtaccgt ccgacggcac cgtgacagta
    22021 aaagaaacgc aatttcctga cggaaaggac gttaccctgc cacgccgaca aaagcgcggc
    22081 agggtgcgcc gtcagcatga agaagacgca gaggaagcac gatgaattca tgcttccctg
    22141 caggtacatt cgccagacgc aatccttcca caatgatggt gccattgccc agcaggattt
    22201 tgtgcaccgc ctcgccccgt ttatacggcc ctatggaaag ataatctatg ccgaccagcc
    22261 tcacaccgtc atcaaccagg cgctgcgcgg catccgcttc aattccggtg aaatcctgaa
    22321 cgaaagagct gtcttcagca atacgggaat tggctgtttt aaaaagcacc ctttcaggaa
    22381 ggcgttcagc aggaagatca gcggcattga tctgatggct tccgctgcag tccagaataa
    22441 gggctgtgcc gaaaaacaat gatttatcta tgttttccag ctgcgctcca ttttcaataa
    22501 aatgccaggg cgcgtccaga tgagtccccg tgtgcgttcc cagggacagc tttgagacat
    22561 tgcatgaatt accccgggca atgcgtacat ccgcttccag cgaaaaagga ggatctccgg
    22621 gccacagagt catcccacgg tgcataggca gggtgacatc aatccatcga agattcattt
    22681 tctttatttc ctgcttttaa tcgatgccac gtacccagtt ttctgtgaaa gcggcatgtt
    22741 ttatagtgct gccctccaca ccggagcgcc ggtaggagta gctgaggtgc agcgtctgat
    22801 ccgcaccctg gataagtgtg ggataagaaa aattgccttc tccttcaggt gcctcttcta
    22861 ttcgacgtga aacgtgccag cttttcccct catcatcgga aagatagact gtcaacaagt
    22921 gtctgcccag tgtggtgtca ttgcagacaa gcagccacac gccgctttga agcgcaaggg
    22981 cacttacgct ggatcccggg ttcggaatgc cgatgaaatc gaccttggac cagtgcatgc
    23041 cctgatcggt agacagagac tgccgaatct gctggggcaa tccgttgtcc cgcataaaag
    23101 cgacaatatc gccgttgctg cgcttgacaa aagccggttg gatgtttcca agcataagca
    23161 gatcatcatc cagaataggt gcgctgctct tccagtgctg accccaatcg gaagtgaata
    23221 cggcaaggga ataattgaaa acatcggaat aaagtccgag catgatggtt gtgtcatcga
    23281 gcataatggg tgtggtgcga cccatccacc caagacgctg ggtcagcttt tcctctccct
    23341 gttttctgat tgccgctatc tggtctttct tttccggtgc caatgtttct attaaggaac
    23401 tgtaagtatt cagcccttca tcaacaacgg ggaaaaaacg ttcttccagg tccagagggc
    23461 gcatatgaat aatgtcctgc cagtcccatt taggagcgcc gtcgccgaga taatcgcgag
    23521 aagtccggta ttttaataga gcactccccc attcgttgtc ctgaatagca gaccagaaaa
    23581 gccaaaggac accgcgggga tcgatgaaca gcacaggatt gcagtctggc agatccggcg
    23641 agtccgccat cagaaatact tcagaccagt ggttatcacc ggcttttttt cgcgcgccct
    23701 gaatcaccac attgtccgct tcccgctcac ctgtaccatg aaaccaggcg gcaaggaggc
    23761 tcccgtccga gcactccaca atactggaac tgtggttatg catttcattt ggaggaaaaa
    23821 tatactccgc ctgaaaagat gcggaaagaa aaagtgttgt gaagaaaaaa gtgaacatgc
    23881 cgttttcctt tatctgtgga cagcggtcgg atcaatgaag actttttcat tatataaaaa
    23941 ggcctgccgg gaaagatacc cgacaggccg cagttggata attcgagaaa ggtcagacgc
    24001 tgagtgactg aattttcgca agtatagagg caggatcaag tccctgatgc ggcatatgct
    24061 cactgagccc gcctttgcca tgttttgtaa ccccgagagc ggcatagcgc ggagtaaagc
    24121 ctctttcaag cagccaggtg ccgaaacggc ttccgaggcc tgttttggcg ttctgacttt
    24181 ccacaaccag cacaaagggc gctttgctca gcaacgccag cgtcttttca tcaggaacat
    24241 tcagggtagg tttattaatc agacctacgt ccagtcctgc ttctcttgcc tgctctacag
    24301 catccagcga gcggctgagc atatctccat aggagacgat atatccctga gtaccggtgc
    24361 ggattatttc atcttttccg ggtacaaact gatatggagc ttcatagaaa cgggacccgt
    24421 cctctttgca gataaaaggt gttgcgctgc ggagcgtaaa gacaaagcgc gcccccttgt
    24481 cattccagat ggtctcgacc aggctcttca gctgcagagc atcggcagcg aaatacagcc
    24541 gtgtggtatc gccttcagcg attgcactgt cggcgagaaa gatattgatt ccgtaatggc
    24601 aggtgttgtc ggccatatca tcaatacccg catgggagaa atggcaaagg acgttggcat
    24661 cattgagccg tgccatagtc agttcggaaa tgatcatttc cagaaatgcg gagaaagtgc
    24721 tgaatatgcc ttgccgccct tctttgaaac cgaagcccgc cgctgcggag aagttgcccc
    24781 gttcctgtac gccaccgttg gtgcagactt caggatgggc gatacggata actttcaggc
    24841 cggtggagcc ttccagatcg gaatcaacga caagtacttt gtcaatgcgc tcagcttccg
    24901 gcatgcgatc cagaattgca ttgacgattt taccgaattc gccgcgattg ctcgccgttt
    24961 ccgctgtaca gcctttgtat accgggttgc ctgatacctt tttcactcct tccaggtagg
    25021 cgatcgcatc agtaagattg cgggctttta agtattccag tgcgggagcc agcgcaatca
    25081 cgtcgtgccc ggcggaagtg ccttccacta caggcacgcc tggtgccata aggcgtttat
    25141 tgatcagggc aacagggcct tcctgacgga cagcggttac catgcgttgg tacagcgaat
    25201 cgaagtcttc cccttccccg acacaggtgg caagtccatg tccttttagg gtggcggcaa
    25261 catcgaatcc gggcaggtac ttggacggat gtccgctgat tgtcacatca ttgtcatcga
    25321 tcagcagttt tgcattcagc tgacggctga cggcaaaacg agcagcctcg gcatcatccc
    25381 cttccatttg cgacccgtcg gagccgagaa gaaaaacaac tttatcaggg ttggcacatg
    25441 ccacaccatt tacaaaaggc cagagatggc ccaggcgacc ggatgcaaat ttgatgccca
    25501 gttccggatc cagttcggga tgtccgtata acccctcata agcggcgcgg tactgcagaa
    25561 gtttctcgaa aggcatttcc ccattgaaag cggagagggc gtattgcagc gctacacggt
    25621 gaccgccttc atcaaaatga acgggataaa agcgatcaga acctttcata aaagcatcgg
    25681 caatgagagc ctccgggacg atactgtaag cgcctccggt gtgtccgccc agtccttttg
    25741 cgcctgctac cgcagtgaag aaaataatgg tatcacgcat tatttgaata tttgtcaaca
    25801 attgagcacg ctgttccgaa gtgatttcat cggtgaaagg attaatcgca acagctttgt
    25861 aagcactgac atctacggga aatgacattt caaaacttcc tt
//
LOCUS       DAOPWB010000033        25643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1901_length_25643_cov_5.448570, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000033 DAOPWB010000000
VERSION     DAOPWB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 25643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25643
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1901_length_25643_cov_5.448570"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKN92_04775"
     CDS             <1..609
                     /locus_tag="PKN92_04775"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNZ48139.1"
                     /translation="YLPLFILALAATVVLWRLHWQWAALPFWFAAFFVLFFFRDLPRQ
                     SDADSSTLISPADGTVVAIEDLEESPHYDGPCRRVSIFMSVFNVHVNRAPFDCTVRDV
                     RYAPGAYINAMKKEASKINESNALWLDTPHGSVTVRQISGAIARRIVCRAQPGKSLTR
                     GEKFGMIKFGSRVEVYLPPETEVLVSLQQKIKSGETPLAKFQ"
     gene            606..1541
                     /gene="pssA"
                     /locus_tag="PKN92_04780"
     CDS             606..1541
                     /gene="pssA"
                     /locus_tag="PKN92_04780"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNZ48140.1"
                     /translation="MTPSLFQKNRTPRRFLRINVVASILTTMNLYMGTTSILASIGQE
                     FQWAATCILLAILFDTFDGFVARLTHSVSDFGKELDSLCDMVSFGVAPAVLVFMAYLP
                     PDAHLPLSPRTESIVGMTGSYMAIIFVICAALRLARFNTWHAGRSDFFIGLPSPAAGG
                     TLAAFVLFLLYFEKSLNAHALGSLAYVALGPTSVFLAFLMVSSVQYPKNRLKTFLFKP
                     RHAFQALAITAFFIGIIHYAMVKSVSLVLFPLACLYVLYGLGDTLYGKVTGREPSNIA
                     REDMEAPENDDVTEVGLQAEGEENEISSGDSLASF"
     gene            1825..5403
                     /gene="nifJ"
                     /locus_tag="PKN92_04785"
     CDS             1825..5403
                     /gene="nifJ"
                     /locus_tag="PKN92_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNZ48141.1"
                     /translation="MTRPMRTMDGNEAVANVAYQVSEVAAIYPITPSSNMGEWADQWM
                     SEGRPNIWGTVPTVVEMQSEGGAAAAVHGALQTGSLTTTFTASQGLLLMIPSMFKIAG
                     ELTPAVFHISARTLATHALSIFGDHSDVMSARSTGFGMLASGSVQEAQDMALIAHAAT
                     LAARIPFMHYFDGFRTSHEENKIEVLQPADMRAMIDDQLVFAHRMRAMSPDRPVLRGT
                     AQNPDVFFTAREACNPFYEACPAIVQQTMDKFAAVVGRQYHLFDYIGAPDAERVIVLM
                     GSGAEAAAETIDYLVQKGEKIGAVIVRLYRPFSAAALLAAFPKTTKVIGVLDRTKEPG
                     AAGEPLFMDVATAVTEATINGNSPFAVLPRIIGGRYGLSSREFTPAMIKSIFDEMCKE
                     EPKRRFTVGINDDVSHTSISYDPSFSTESPETTRALFYGLGADGTVGANKNSIKILGE
                     DAGFYAQGYFVYDSRKSGSMTTSHLRFGPKPIRSTYLITRANFVACHQFSFMEKFDVL
                     RCADEGAVFLLNSIYGPDEVWDHLPKTAQRQIIEKKLKFFIINGFEVAKAAGMGGRIN
                     TVMQTCFFYISGVMPQAAAIEAIKTSIKNTYGKRGDTIVQKNWAAVDMAVEHLHEVKV
                     PGAVTSTFDIRPAVPAHAPEFLHEFTAAILAESGETLPVSKMPVDGTFPTGTTQWEKR
                     NIALEIPEWDEEICIQCGKCALVCPHGVIRAKIVEEETLADAPEGFKSARAKWKEFAD
                     NRYIMQIAPEDCTGCALCVQACPVKNKQQPKLRAINMVEQAPIRDRERERWNYFLSLP
                     EVDRGAVKMNLVKDVQLLRPLFEFSGACAGCGETPYVKLISQLFGDRLYVANATGCSS
                     IYGGNLPTTPWAFNADGRGPAWNNSLFEDAAEFGLGMRVTIDKQKEFAEELVKRLESQ
                     IGGTLAAELLSAEQKTEAGIQAQRERVAALKEKLAAIGTGEAKVLAHVADFLVRKTVW
                     SMGGDGWAYDIGYGGLDHVIASGKDINLLVMDTEVYSNTGGQCSKATPRGAVAKFAAG
                     GKPGSKKDLGLIAMTYGNVYVAQIAMGANDSHTIRAILEAEAYEGPSIVIAYSHCIAH
                     GYNLVMGLEQQKNAVQSGHWPLYRFNPALKAEGKNPFQLDCKEPALDLDKYIYNETRY
                     KMLTLSQPELAASLLEGAKEDVAARWRLYSYLAKMEFGSENETAVE"
     gene            5497..6495
                     /locus_tag="PKN92_04790"
     CDS             5497..6495
                     /locus_tag="PKN92_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015079866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNZ48142.1"
                     /translation="MDLRTNYLGMELKNPIVVSPSPLTENLDNMKKMEDAGAAAIVMH
                     SLFEEQITFMSEDLNENLQQGTMSFSEALTFFPEEQDYKMGPDSYLEHIRKAKEMVNI
                     PVIGSLNGVSVGGWQDIATKIEQAGADALELNVYYIPTNPEQDGPHVENLYLDLMREV
                     KKAITIPVAVKIGPYFSSTANMVQKLDQAGADGLVMFNRFYQPDLDLDNLEVKPDLTL
                     SSSWELRLALRWVAILYSRIQADMAITGGVHTGTDALKAMTAGAKVAMMTSAILRKGI
                     DHINTVLAEMTQWMEAHEYESIAQMQGSLSQRNCPNPAAFERANYMKIITCYTPNA"
     gene            6810..7913
                     /locus_tag="PKN92_04795"
     CDS             6810..7913
                     /locus_tag="PKN92_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48143.1"
                     /translation="MLPLPRLLLFFFASALLCLPSLGPLSAQSTPYPREHWEEASPEA
                     MGLERDALDKLAAYMEGDGCVIRHGRMVYRWGDPTRRGDVASACKPWISFFLFLALEE
                     GLLEGLDMPAHHYKPCLAEINAELGYKDRGILMRHFATQTSCYGVRETPGTAFDYNDW
                     QTALFVEILFQQIYKVPPDGYDGHILLPRLTEKLQCEDSPTFYAFGPQDRPGRLAVSP
                     RDFCRFGYLFLQRGHWHSGRILAGEHIRKLTTQPLPAALPRTRAQEAALCEGARSHGS
                     QRIPDDQCDHGGSYSWMWWVNGVDREGRRYWCDAPDDTFAALGHKNGMRGMAVIPSLD
                     IVFSWNNTRLGTLPESSDVLNDAFRYLCSAVKN"
     assembly_gap    8106..8205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8206..>8643)
                     /locus_tag="PKN92_04800"
     CDS             complement(<8206..>8643)
                     /locus_tag="PKN92_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48144.1"
                     /translation="TKTGTWVYGERHPTIDAHDKKRSSSTASSSTLWHSNSSTSSDNS
                     SNSSSSNSSGSSLYSSPSTYSSSFGSSETTSTSKKNSASGSSLYSSPSTYSSGFGSSG
                     STSTSKKNSASGSSLYSSPSTYSSSFGSSTPPASTSSTFGSSD"
     assembly_gap    8644..8743
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8744..9068)
                     /locus_tag="PKN92_04805"
     CDS             complement(<8744..9068)
                     /locus_tag="PKN92_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48145.1"
                     /translation="MRTAVTSIFLFVLFVCPAGVWAEDYKPGIEIIHQYRNTDGSYDT
                     YNLSRGEHTFGFRNPDGSQDTYNTTTGEHTFGFRNSDGSQDTYNTKTGEHTFGFRNSD
                     GSQDTY"
     gene            9404..9952
                     /locus_tag="PKN92_04810"
     CDS             9404..9952
                     /locus_tag="PKN92_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNZ48146.1"
                     /translation="MLTVTIRGLIQSEESGVPSVILTSSDDVLLISIGVAEAASIQMA
                     LEGKKLPRPMTHDLIENLLSGLHATLRSVTIYKIQNSTFYAYLSVEQRNDQDDVIQEL
                     RIDARPSDGIAIAVRVGCPVYVAEDVMKQAGLRADFFFGQLDLNQKVEEENNIFFIED
                     FDLDMFDDEDDDDEDDDEGGFR"
     gene            10082..10912
                     /locus_tag="PKN92_04815"
     CDS             10082..10912
                     /locus_tag="PKN92_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ48147.1"
                     /translation="MTREYLPGTEIEIIRQVERGKIKHILFDFDGTISLLREGWQGIM
                     APVCVEMICGSTEPTDQIREEVNVMIDETTGIQTIFQMQRLQEMVRAHGLVPEAEIKS
                     PEAYKEVYNDRLMVPVRERIAAIKSGARTRESFTVKGSLRFLELLSRLDVTMYVFSGT
                     DRDDVRNEASCLGVADYFNEIWGALPSVEEYSKEKIIREIISQHHLEGPQVLAIGDGP
                     VELRNVRDAGGIALGIASDEVNGEGWDESKRDRLLKAGADMLVPDFKEAEQLVEYLFP
                     "
     gene            11032..12504
                     /gene="guaB"
                     /locus_tag="PKN92_04820"
     CDS             11032..12504
                     /gene="guaB"
                     /locus_tag="PKN92_04820"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNZ48148.1"
                     /translation="MTSQNNIQDGLSFDDVLLVPARSAYTPRDVQIQSRLTRTLGLNI
                     PLLSAAMDTVTESKLAISIAQEGGMGIIHKNLSAEEQAEEVDRVKRSQAGIITDPFTL
                     RADQKLSDAEALMSRYHVSGVPITDETGYLVGILTNRDLRFEEDMNVAISTIMTPRER
                     LITMPPNTDMESAKKLLHKHRIEKLPLVDDGGRLVGLLTIKDIMKARDFPNRNADAMG
                     RLRVGAALGVGDDEMERAKALLQAGADVLVLDSAHGHSELIIEMVRQIKRSFPSAQLV
                     AGNIVTAEAAEDLIDAGADAVKVGIGPGAICTTRVIAGVGVPQLTAILNVASVCREAD
                     IPVIADGGIKYSGDIAKAIAAGADSIMIGSLFAGTEESPGEKVLYEGRTYKLVRGMGS
                     LKAMQRGSKARYMQFETDASKLVPEGIEARVPYRGNLANYVFQLMGGLRAAMGYCGCK
                     DIPSMQRKARFVRMTSAGIRESHPHDVTITEDAPNYQIPS"
     gene            12530..13786
                     /gene="guaA"
                     /locus_tag="PKN92_04825"
     CDS             12530..13786
                     /gene="guaA"
                     /locus_tag="PKN92_04825"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNZ48149.1"
                     /translation="MQNSLKPIVVLDFGKPDGMQLVRRLREKEVYTLILPAAADVQLL
                     QQITPAGLICFGADKAEQIPPRIAALGLPSFSIAADEEPTPELFAFAASCCGESVPWN
                     MERYASFMLEDIRQRVGSRKVLCGLSGGVDSSVVAALIHHAIGDQLTCVLVDTGLLRK
                     GEADLVREVFEGTFKTKLICVRAEDRFLSALKDVTEPEQKRKIIGALFIEVFEEEAKK
                     LGHMDFLAQGTLYPDVIESYQAAAGASVAVKSHHNVGGLPERMQMALLEPVRDLFKDE
                     VRALGKVLQLPDMLVGRHPFPGPGLGVRCVGAITKDRLDTLRQADAIFIEEIRNAGLY
                     DQIWQALVCLLPVKSVGIKDGKRVWEEVCTLRAINSVDAMTAQPALLPAPLLTKTVER
                     ICTEVPGVSRVLHDISPKPPATIEWE"
     gene            14018..14092
                     /locus_tag="PKN92_04830"
     tRNA            14018..14092
                     /locus_tag="PKN92_04830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14050..14052,aa:Gly,seq:gcc)
     gene            14113..14188
                     /locus_tag="PKN92_04835"
     tRNA            14113..14188
                     /locus_tag="PKN92_04835"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14146..14148,aa:Lys,seq:ctt)
     gene            14303..14387
                     /locus_tag="PKN92_04840"
     tRNA            14303..14387
                     /locus_tag="PKN92_04840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14337..14339,aa:Leu,seq:tag)
     gene            14418..15929
                     /gene="tig"
                     /locus_tag="PKN92_04845"
     CDS             14418..15929
                     /gene="tig"
                     /locus_tag="PKN92_04845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNZ48150.1"
                     /translation="MTEKDVDAMETSEQESTAAAVAEPDETGETDERLVADVEQDDFD
                     WKKPPVFDIAHKEDCVCEVKVTIPAENVMAVLDNVYNEMNDGVQVPGFRRGKAPRKLL
                     EKRLGKYAHSSAIEQLTDAAAKRLVLESGLRPVSTAEVTGLEDKEQLNDQEDLVYTIT
                     FEVPGTCALADLAEIELERPEYEVSEEALESSINNIRTRFGRFEPLEDGVAEEGDQVV
                     IDFKGTIDGEAFEGGSAEGYPYILGSKRFDENMEAAMVGKKAGDTATAEVVFADDYAA
                     AEVAGKTAQFEISINEIKRRVLPELNDEFAKKAGHESMEVMRETMRKRLAETADEEIG
                     HLLEEQVMQKLVDASTFVLPKGQIQKFIDAERASILERMRQQHISAEDMEREEENIRE
                     ASEKQGLFSLKAMYVIDALSEQEKIEIVEQDFDDYARQFVQGDNAEQLEAFRAYFQSD
                     DMRSSTEHRILYSKTLKAAIAKCKIKVVSPEELSKKEAEADTDDAAKEENNDA"
     gene            15973..16587
                     /locus_tag="PKN92_04850"
     CDS             15973..16587
                     /locus_tag="PKN92_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNZ48151.1"
                     /translation="MSDPKPVSPEAVTIYQQTSRGERAYDIFSRLLEDRIIFLGYPID
                     DAVANYIIAQMLFLEAEDPDKPINIYINSPGGSVTAGLAIYDTMQYIRPEITTICIGQ
                     AASMGAVLMAAGTKGRRFALPYSRFLLHQIMGGVRGQATDIDIQAREIVRISEMTDEI
                     LVRHTGQPLEVIRRDSDRDFFMGAEEAKEYGLVDEVLHRLPTGA"
     gene            16772..18058
                     /gene="clpX"
                     /locus_tag="PKN92_04855"
     CDS             16772..18058
                     /gene="clpX"
                     /locus_tag="PKN92_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNZ48152.1"
                     /translation="MASQQGSRRKGPTCSFCGKSHAEVNKLIAGPDVNICDECVSLCI
                     DIVAADQGRIKKRKASHVPTPQEIKDFLDQYVIGQDQAKRILSVAVHNHYKRLAADSE
                     VDGVELQKTNVLLIGPSGCGKTLLAESLARMLDVPFAIVDATTLTEAGYVGDDVENIV
                     LKLLQSADFDHERAATGIIYIDEIDKIARKSDSPSITRDVSGEGVQQALLKIIEGTVA
                     NVPPQGGRKHPQQECVQVDTRDILFICGGAFNGLEQIIRKRTGNNAIGFNAEIKSKDR
                     KRLGDILAQARPEDLISFGMIPEFSGRIPVIATLYDLTSDDLEKILTEPKNALIRQYQ
                     KMFQMDNVELQFQPETIRAVAEKAISLETGARGLRAILEEAMLDLMFEIPSRKDIKEV
                     IMTPGAILNREEPIVVYEQEKRKSAPKNQNSSSASA"
     gene            complement(18210..19151)
                     /locus_tag="PKN92_04860"
     CDS             complement(18210..19151)
                     /locus_tag="PKN92_04860"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ48153.1"
                     /translation="MKSNLSRSTLFRRIGITLLLFIITAAAVLAMRWGKVVVETTDQT
                     RSPFPSDAEEVVFTICTYNVQARPLFDETKNKFAHISPLLNRFDICALQECFKDYRRA
                     WAAAEHSSKIFHGTLKHPLKLVGSGLSLLSSLPLISTDNINFDTCGEFQNRPASKGVL
                     LGRYQAGAMTLDVYTTHIEAGGSPQALAARKGQGEEIVDFVNAQSPPENSVIILGDFN
                     MRPSRGPEDKEKHKDNPKVYVFDWIKETLDLRDASDEFNGPVSKEIDRILFRPGKGCA
                     MKVLHWQQDDPDFYYADGKTPLSDHAPLFARFQLERS"
     gene            complement(19245..19742)
                     /locus_tag="PKN92_04865"
     CDS             complement(19245..19742)
                     /locus_tag="PKN92_04865"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNZ48154.1"
                     /translation="MMDALTAMRLRRTVREFEPGKSISKETLETIADAGRVAATAINI
                     QPWEFVIITNRDMLKKMADLAEYGKHLASASAAIAVLCKDSPFYLEDGCAATQNMLVA
                     ATALGIASAWIDGEKKEYGAAMKQLLGAPAEIKLVSIIALGYSNDETTPEKRPLEEVI
                     HWEKF"
     gene            complement(19813..20715)
                     /locus_tag="PKN92_04870"
     CDS             complement(19813..20715)
                     /locus_tag="PKN92_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48155.1"
                     /translation="MMVKNALNPMFLLLTLMLLAAFSGTAAETKKSSDTFPPSKVEVR
                     VFSPEQVAKPDLVPDAAGEDAAKAPTDPLDKVLLEIQVVRDELRMLQSTLDLMINHIM
                     ADMQEENRVLREEVQRLYLIQKEFGIPDTTHIPRPGIGIIRGLLQAVPGMEEKDTPVT
                     PEQSGASFLPDDGGQVPEEADSGAPAEPASLTILHEWGRSPEMVEELDHAVSTLKGLV
                     GVVPPGSRRADVEELGRQLRARYDDYENINIEIFDDQKSAENYVDTQVGDPDHRVLSI
                     SRHSASGRDLILYLDKGEAKEVSP"
     gene            complement(20815..21525)
                     /locus_tag="PKN92_04875"
     CDS             complement(20815..21525)
                     /locus_tag="PKN92_04875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ48156.1"
                     /translation="MYIRAVTFDFWGTLFRDAQGEERHGLRLRGLVEATGAPEQDADR
                     ALRSTMAFFMRNHLDTQHTLTPHDAAQLCCRQLNVHADETTLKQLAQCFGDAILKYPP
                     VPIDRALDAVRVASERVPVALISDTGFSPGRNLRILLDNAGFLSYFSALNFSDETGAA
                     KPQRRMYVSIADRLGIAPHELLHIGDLEPTDITGAHQVHAKAALITAVNARYAGSTRA
                     DYLLPSWDRFLELVPRLL"
     gene            21645..23771
                     /locus_tag="PKN92_04880"
     CDS             21645..23771
                     /locus_tag="PKN92_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNZ48157.1"
                     /translation="MPAFALRPGSIATLICLLGFISPTLSSTADYGAAVETVIEHDDG
                     EWLWFHPRAAAAPGFGRDGAPAVVMTLQKHLQISDYYSGLYALYSDDLGGSWTGPVEI
                     PELAWRDGPGGTTIAVCDVTPGWHAPTGKVLALGAQICYSPDGRQVAQEERFDQTAYA
                     VYDPAKKSWTSWKILEMPDDPKFHCSRNACSQWLTEDNGDLLVPLYYGAAGQSDYSVT
                     VARCRFDGETLRYLCHGTEMTVPGGRGLCEPSLIRFQNRYYLTLRNDARGYVAVSDDG
                     LHFEVPKIWRFDDGAELGSYNTQQHWLASGDTLYLVYTRRGADNDHIIRHRAPLFMAQ
                     VDTERCCVIRQSERVLIPERGAAMGNFGASAMTESEAWVTVGEGVWNDDMRKRGAKGA
                     LLIARIDWNSSRENQQMKAAEVLAASLEPCAQKPFITTLFSKEPLVCCGTSWYCAASP
                     GLPEKLVIMPVLRPEGIIRMDTGASLEYQYEEAEGKGGMVTVPLKSCRDLQQGICIQW
                     GENRWQKNMDQFAEDNLNRTPMRGGMLMTGSSTARMWDVERCFPGRQVLNRGFGGSCY
                     RDLFRFADEVIAGHRPDTVVIYDGDNDIAGGLTPVQTAADCRASVERIRALLPDSRII
                     VLATKPSRSRWNLHEAMFASNAAIAQQLSALPNVQFLDLIPLIMDGEGMPDPVCFKED
                     ALHLNDEGYRRWSAALEKYLPAEKKE"
     gene            23805..25040
                     /locus_tag="PKN92_04885"
     CDS             23805..25040
                     /locus_tag="PKN92_04885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ48158.1"
                     /translation="MLRRLLINSLLVLFSLTLTVLAVLGADRLLGILKAPPDLPKWVE
                     LNFPAGDEQHYSTSEFSYDVYINSLGLRDRELPRERGKAFRIAAIGDSYTYGWGVNIE
                     DCWTSLLNEHFREAGETVEVINLGKPGQGPPEYAALAEKAIPLLRPDLVLVCMLQGND
                     IRGVGPEPDALPAPDFYERVRRCFPNTTRLVRDLQREREYTGRSHLAMPPGRSTAEDN
                     RRWMANTAHEIYEKYSDEEKARFDSFEEPVRTAFMQGDLNAYMVNLSVLNPDFYIMTM
                     DLEDAWTETCISRAAHALSRIRKVAEDYGARTVVVAMPEGPYVNRAALEGMRRIGHNM
                     PEWLLQTDAMDKTIETAATRAGLPCYKVTDAFRKEIDRTDLYFPLDGHPTAAGHLLFK
                     EAFLPALEKLIAGQKKGGQ"
     gene            complement(25257..>25643)
                     /locus_tag="PKN92_04890"
     CDS             complement(25257..>25643)
                     /locus_tag="PKN92_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48159.1"
                     /translation="VEGETPVEGETPVEGETPVEGETPVEGETPVEGETPVEGETPVE
                     GETPVEGETPVEGEPVIEGEPVIEGEPVTEGESVIEGEDSNTGSGCGCKSKDLRTSTK
                     ALLGDWLLLGLCLSTLLAGSALRKKI"
BASE COUNT         5867 a         6567 c         7077 g         5932 t
ORIGIN      
        1 tatcttccgc tttttatcct cgcacttgcc gcaacggttg tcctgtggcg gctgcactgg
       61 cagtgggcgg cgcttccttt ctggtttgcc gctttttttg ttcttttctt ctttcgcgat
      121 ctcccgaggc aaagtgatgc ggattcttcc acgctcattt ctcctgccga tggtactgtg
      181 gtcgccatag aggatctgga agaatcaccg cactacgacg ggccgtgccg gcgtgtgtcc
      241 atttttatgt ctgtctttaa tgtgcatgtt aaccgggcac ctttcgactg tacagtgcgg
      301 gatgtccgtt atgcacccgg cgcttatata aatgccatga aaaaagaagc gagcaagatc
      361 aacgaatcca atgctctgtg gcttgacaca ccgcatggtt ccgtgacggt gcggcagatt
      421 tccggagcca ttgcacgtcg cattgtctgc cgtgcgcagc cgggcaaatc attgacacgc
      481 ggcgaaaaat tcggcatgat taagttcggc tcccgtgtgg aagtgtatct tccgcccgaa
      541 acagaagtgc tggtttctct ccagcaaaaa atcaaatccg gcgagacgcc gttggcgaaa
      601 ttccaatgac tccttcactt tttcagaaaa atcgcactcc ccgccgcttt ctccgcatta
      661 atgtggtggc aagcattctg actaccatga atctgtacat gggcacgacc agcatactgg
      721 caagcatcgg tcaggaattt cagtgggcgg caacatgtat cctgctggca attctcttcg
      781 atacctttga cggatttgtt gcacggctca cgcacagtgt gtccgacttc ggcaaggaac
      841 tggacagtct ctgcgatatg gtttcctttg gtgtcgcccc tgctgtgctc gtgttcatgg
      901 cgtatctgcc gcccgatgca catctccccc tgtcgccccg aacggaatct attgtcggta
      961 tgacaggatc gtacatggct atcatttttg ttatctgtgc ggcattgagg ctggcacgct
     1021 tcaatacctg gcatgccggg cgttcggatt tctttatcgg gctgccgtct ccggcagctg
     1081 gaggtaccct tgcggctttt gtactcttcc ttttatattt tgagaaaagc ctcaatgctc
     1141 atgcgctggg atcacttgcc tatgtcgctc tgggaccgac ttccgttttt ctcgctttcc
     1201 tgatggtcag ctccgtgcag tatcccaaaa acaggctgaa gacatttctt ttcaaaccgc
     1261 gtcatgcatt tcaggcgctc gctataacag cgttttttat tgggatcatt cattacgcca
     1321 tggtcaaaag cgtgtctctt gtgctgtttc cgctggcatg cctctatgtc ctgtacgggc
     1381 tgggggatac cttgtatggc aaagtgacgg gccgggagcc gtcgaatatc gccagagaag
     1441 acatggaagc acctgaaaat gacgacgtca cggaggtcgg ccttcaggca gaaggggagg
     1501 aaaacgaaat ctccagtggc gactccctcg cctcctttta gttttttctc cgatgcgcca
     1561 cgtctctaaa attaactctt ttcttgttta ctatgcttaa cgaatgatta gacctgtcct
     1621 tgcttatacg gagaataaac agcataatat gcgtattccc gcaacctgac aggtgcccct
     1681 gatctgcttt gcaaaaaagc agatcgatat gggggctttt ctgctgatca ggggaaaata
     1741 atttctcttt tgcagaaaaa agcgggcaaa cctcttgata cctgatcaat gcagtgctac
     1801 gacctacaga ttggagttaa aaacatgacc agaccgatgc gtaccatgga cggtaatgaa
     1861 gcggttgcaa atgtagctta ccaggtaagc gaagtggcag ccatttaccc gattacccct
     1921 tcttcgaaca tgggagaatg ggcggatcag tggatgtctg aaggcaggcc caatatttgg
     1981 ggaacggtgc ctactgtagt tgaaatgcag agcgagggcg gcgctgccgc agccgttcat
     2041 ggcgcactgc agacaggatc cctgacaact actttcacgg catcgcaggg tctgctgctc
     2101 atgattccga gcatgtttaa aatcgcaggc gagctgaccc ctgccgtatt tcatataagt
     2161 gcccgtaccc tggcgaccca tgcgctgtcc attttcggcg atcacagtga cgtcatgtcc
     2221 gcccgcagca ccggattcgg tatgcttgcc tccggttccg tccaggaagc ccaggacatg
     2281 gcgctgattg cccatgcagc caccctggca gcccggatac ccttcatgca ctacttcgac
     2341 ggtttccgta cgtcccacga agaaaacaaa attgaagtgc tgcagcccgc cgacatgcgt
     2401 gccatgattg atgaccaact ggtctttgcc caccgcatgc gcgcaatgtc gccggatcgt
     2461 cccgtgctcc gcggtactgc ccagaacccc gacgtgttct ttaccgcccg cgaggcctgc
     2521 aatccttttt atgaagcctg ccccgccatt gtgcagcaga ccatggataa atttgctgcc
     2581 gtagtcggcc gtcagtatca cctttttgat tatatcggcg ctccagatgc ggagcgggtg
     2641 attgtgctta tgggttccgg agcggaagcg gcagcggaaa ccattgatta tctcgtgcag
     2701 aaaggcgaga aaatcggtgc cgttattgtg cgtctctatc gtccgttctc cgcagccgcc
     2761 cttcttgctg ccttcccgaa aacaacgaaa gtgatcggcg ttcttgacag gaccaaagag
     2821 cccggagccg caggcgaacc tctgttcatg gatgttgcca ctgctgttac ggaagcaacg
     2881 atcaacggca actctccctt cgcagtgctg ccccgcatta tcggcggtcg ttatggtctt
     2941 tcctcccgcg aatttactcc tgccatgatc aagagtattt ttgacgagat gtgcaaagag
     3001 gagccgaaac ggcgctttac tgtcggtatt aacgacgatg tttcccatac cagtatttcc
     3061 tatgatccct ccttctccac ggagtcgccg gaaaccaccc gtgcgctctt ctacggtctg
     3121 ggtgcggacg gcactgtggg cgccaataaa aactccatca aaattcttgg tgaagacgca
     3181 ggtttctatg cgcagggcta ttttgtgtat gactcgcgca agtccgggtc catgaccaca
     3241 tctcacctgc gtttcgggcc caagcccatc cggagcacct atctgattac ccgtgcgaac
     3301 tttgtcgcct gccatcagtt ttctttcatg gagaaatttg atgtgctgcg ctgcgccgat
     3361 gaaggcgctg tattcctgct gaacagcatc tacggaccgg atgaggtctg ggatcatctg
     3421 ccgaagacag cccagcggca gatcattgag aagaaactta aattcttcat tattaacggc
     3481 ttcgaagtgg caaaagcggc cggtatgggc ggacgtatca ataccgtcat gcagacctgc
     3541 ttcttctata tcagtggtgt gatgccgcag gcggctgcca ttgaagcgat taaaacctcc
     3601 attaaaaaca cctacggcaa gcggggcgac accattgttc agaagaactg ggccgctgtg
     3661 gatatggcag tggaacatct gcatgaagtg aaagtgcccg gcgccgtaac cagcaccttc
     3721 gatatccgtc ccgcagtgcc cgcccatgct ccggaatttt tgcatgaatt caccgctgcc
     3781 attctggcgg aaagcggcga gacccttccg gtaagcaaaa tgcccgtaga tggtaccttc
     3841 cccacaggga cgacccagtg ggaaaagcgg aacattgccc ttgaaattcc tgagtgggac
     3901 gaagagatct gtatccagtg cggtaagtgc gctctcgtgt gtccccacgg cgtaattcgc
     3961 gccaaaattg tggaagaaga aaccctggcg gatgcgcctg aaggctttaa gtcggcgcgc
     4021 gccaaatgga aagaattcgc cgataaccgc tatatcatgc agattgcccc ggaagactgc
     4081 acaggctgtg cgctctgtgt ccaggcgtgt ccggtgaaaa ataaacagca gcccaagctg
     4141 cgggcgatca atatggtgga acaggcaccc atccgtgacc gggagagaga acgctggaat
     4201 tacttcctgt cactgcccga agtggatcgt ggtgccgtca agatgaatct cgtaaaagat
     4261 gttcagctgc tgcggccgct ttttgaattc agcggtgcct gtgcaggctg cggcgaaaca
     4321 ccctatgtga agctgatttc ccagctcttc ggcgatcgcc tgtatgtcgc caatgccacg
     4381 ggctgctctt ccatttacgg cggtaatctg ccgaccacgc cctgggcttt taatgcggac
     4441 ggacgcggcc cggcgtggaa caactccctc tttgaagatg cagcggagtt cgggctcggg
     4501 atgcgtgtga ccattgacaa acagaaagaa tttgctgaag aactggtcaa acggctggaa
     4561 agccagatcg gcggaaccct ggctgctgaa cttctcagcg ctgaacagaa aaccgaagca
     4621 ggcattcagg cgcagcgcga acgggttgca gcactgaagg aaaaactggc ggccatcggc
     4681 actggtgaag ccaaagtgct tgcccatgtg gcggatttcc tggtgcggaa gacggtgtgg
     4741 tccatgggcg gtgacggctg ggcgtatgac atcggctatg gcggtctgga tcatgttatt
     4801 gcttccggca aagacatcaa cctgcttgtg atggatacgg aagtgtattc gaatacgggc
     4861 gggcaatgct ccaaagccac gccgcgcggt gccgtcgcca agtttgcggc aggcggaaag
     4921 cccggctcca aaaaagatct gggacttatt gctatgactt acggcaacgt ttatgttgcg
     4981 cagattgcca tgggtgccaa tgattcccat acaatccgtg ccattctcga agctgaagcc
     5041 tatgaaggac cttccatagt catcgcctac agtcactgta ttgctcacgg ctacaatctg
     5101 gtgatgggtc tcgaacaaca gaaaaatgct gtccagtccg gtcactggcc cttgtaccgc
     5161 ttcaatcctg cactgaaagc ggaaggcaag aatcccttcc agctcgactg caaagagccg
     5221 gcgctggatc ttgacaaata catttataat gaaacacgct ataagatgct gacgctcagc
     5281 cagcccgaac ttgcggcttc ccttctggaa ggggcaaaag aggacgtggc tgccagatgg
     5341 cgcctctaca gctatcttgc gaagatggag tttggttccg aaaacgagac cgcggttgaa
     5401 taatctgccg gtgcttttgt cggtacagtt gagtataaaa acccaaacct gcaaatccgg
     5461 cacgccccgg gcgtgacacg gaggaggaaa agatacatgg atctgcgtac aaattacctg
     5521 ggcatggaac tgaaaaatcc catcgttgtg tccccgtctc cccttacgga aaatttggac
     5581 aacatgaaaa agatggagga tgccggagca gccgccatcg tcatgcattc cctctttgaa
     5641 gagcagatta ccttcatgag cgaagatctc aacgaaaacc tgcagcaggg aacaatgagc
     5701 ttttccgaag cgctcacttt cttccctgag gagcaggatt ataagatggg tcctgattcc
     5761 tatctggagc atatccggaa ggcaaaagaa atggtgaaca ttcctgtgat cggcagcctc
     5821 aacggcgtga gcgtcggcgg ctggcaggat atagccacca agatcgagca ggcaggcgct
     5881 gatgccctcg aactgaatgt gtactatatt ccgaccaacc ccgaacagga tggcccgcac
     5941 gttgaaaatc tgtatctgga tctcatgcgg gaagtcaaaa aagcgatcac cattcctgtt
     6001 gccgtcaaga tcgggcccta tttcagctcc accgccaata tggtgcagaa actggatcag
     6061 gcaggtgccg acggactggt tatgttcaat cgcttttatc agcctgacct tgatctggat
     6121 aaccttgagg ttaagcccga cctcaccctc agctcttcct gggaattgcg cctggcattg
     6181 cgttgggtag ctattctgta cagccgcatt caggcggata tggcgatcac aggcggcgtg
     6241 cacaccggaa cagatgcgct caaagcaatg acggcaggag cgaaagtcgc aatgatgacc
     6301 tccgccattc tccgtaaggg aattgaccac atcaatacgg tgcttgccga gatgacacag
     6361 tggatggaag cccatgagta tgaatccatc gctcagatgc agggcagtct cagccagcgc
     6421 aactgcccga acccggcagc ctttgaacgc gccaattaca tgaaaatcat tacctgctat
     6481 acaccgaacg cataagtggt tttctacacg atcaatcctg ccccggtcat gacgttgtca
     6541 cggccggggt ttctttttgc tgctgctgct gcgccttatt gaagtctgga gagcgccgga
     6601 aaatgcacaa cagatataac ggcacgctct tttcctccat ccccccagaa aatccgcttt
     6661 ctcacccggc agacaccatt tccggtggct gacttttccc gagaaggtgt aagtccatgc
     6721 gctcaaaaag atttcgccgg ttcttctcct tccggctttc atgctacact cttctttgtg
     6781 cgtatttttg tcctagggag aacatggcta tgctgccctt gcctcgactt ctgcttttct
     6841 tctttgcttc tgctctttta tgtctgccgt ctctaggtcc actgtcagcg cagagcacgc
     6901 cctatccgcg cgaacactgg gaagaagcat ctccggaggc aatgggcctg gagcgcgatg
     6961 cgctggacaa gctcgccgct tatatggagg gggacggttg tgtgatccgc cacgggcgca
     7021 tggtataccg gtggggcgat ccgacccggc ggggtgatgt agcatcggca tgtaagccct
     7081 ggatcagttt tttccttttt ctggctctgg aggaagggct tttagagggc cttgatatgc
     7141 ctgctcatca ctacaagccc tgtctggcgg agatcaacgc cgaactgggc tataaagacc
     7201 ggggcattct gatgcgacat tttgccacac aaacctcctg ctacggagtg agggaaacac
     7261 cgggtaccgc tttcgattac aacgactggc agacggcgct ttttgttgag attcttttcc
     7321 agcaaattta caaagtgccg cccgatggtt acgacggaca cattcttttg ccacgtctga
     7381 ccgaaaaact tcaatgtgag gattctccta ccttctatgc cttcggtcct caggacagac
     7441 cgggcaggct cgccgtgtcg ccccgcgatt tctgccgttt cggttatctt tttcttcaga
     7501 gaggacactg gcacagcgga cgcattcttg ccggggagca tatccggaaa ttgaccacac
     7561 agcccctgcc tgctgcattg ccccgcaccc gggcgcagga agcagcgctc tgtgaaggtg
     7621 cgcgcagtca tgggtcacag cgtatccctg acgaccagtg cgaccatggg ggcagctaca
     7681 gctggatgtg gtgggtgaac ggtgtggatc gggaaggcag gcgctactgg tgtgatgcgc
     7741 ccgacgatac ctttgctgcg ctggggcaca aaaacgggat gcgcggtatg gcggtgatcc
     7801 cgagcctcga cattgttttt tcctggaaca atacccgtct tggcacgctg cccgaaagtt
     7861 ccgatgtcct gaatgatgcc tttcggtatc tatgttctgc tgtgaagaac tgatatcttt
     7921 ttaaaaaaga aatgcccggc tttccttcag acttgccttt tccctttgcg caggatactt
     7981 gcttaatgga cagcgcagca tgccaaaggc gtaattaatc ttccttagtc atcggatccc
     8041 cagctattcc agtcggaact gccgaaggta gaggaggttg aggcaggagg cgtggaagag
     8101 ccgaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagtcg gaactgccga
     8221 aggtagagga ggttgaggca ggaggcgtgg aagagccgaa actgcttgaa taagtggaag
     8281 gggaagaata cagggacgag ccggaggcgg agttcttttt ggaagtgcta gtacttccag
     8341 aagagccgaa gccgcttgaa taagtggaag gggaagaata cagggatgag ccggaggcgg
     8401 agttcttttt ggaagtgctt gtggtttctg aagagccgaa actgcttgaa taagtggaag
     8461 gggaagaata cagggacgag ccggacgagt ttgagctgct ggagttgctg gagttgtctg
     8521 agctggtgct gctgttggaa tgccaaagcg tggaggacga ggcagtggaa gaagatctct
     8581 ttttatcgtg cgcatcaatg gtggggtggc gttcaccata gacccacgtg ccggtttttg
     8641 tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgtaggt atcctgtgag
     8761 ccatcagaat ttctgaatcc aaaggtatgc tcgccggttt ttgtattgta ggtatcctgt
     8821 gagccatcag aatttctgaa tccaaaggta tgctcgccgg tggttgtatt gtaggtatcc
     8881 tgcgagccgt caggatttct gaatccaaag gtatgctcgc cccttgataa gttataggtg
     8941 tcgtaggagc catcagtatt cctgtactga tggatgattt ctatgcccgg cttgtagtcc
     9001 tctgcccaca cgcctgccgg gcagacaaac aggacgaaga gaaaaatcga agtgactgca
     9061 gttctcatga aatacccctt tcggttaaag ttccgctcag cccagtgcga cggtgttgtt
     9121 tcactgtttg ccctattata acacactgat atatggaatg cttcagaaat gacgccagga
     9181 caagtgtact gttttgccaa aggagctgcc caggcagaac ggcgaggaag gtatgtacca
     9241 actgggctga agtgcatgct tcatgcgaca tgccgcttct tttcactttg aaaaagggtg
     9301 tttcaaaagc aaatcagttc ggctcgaaaa gataatttcg aagtggcatg tcccttcagg
     9361 tagaatatac ttgttctttc gaaagctcag ggatcttgtg aaaatgctga ccgttaccat
     9421 cagaggtctt atccaatccg aagaatcggg cgtgccaagc gtcattctta cgagcagcga
     9481 tgatgtgctt ctgatttcca tcggcgttgc cgaagcggct tccatccaaa tggcgttgga
     9541 agggaaaaaa ctgccacggc ccatgaccca tgacctgatt gagaatctat taagtggtct
     9601 gcatgccacg ctccgatccg tgaccattta taaaatccaa aattcaacct tttatgctta
     9661 tctcagcgtt gagcagcgca atgaccagga cgacgtgatt caggaattgc gcattgatgc
     9721 gcgacccagc gacggcattg cgatcgctgt tcgtgtcggc tgtccggtct acgttgcaga
     9781 agacgtgatg aaacaggctg ggcttcgcgc cgatttcttt ttcggtcagt tggatctgaa
     9841 tcagaaggtt gaagaagaga acaatatttt cttcatcgag gacttcgatc tcgatatgtt
     9901 tgacgacgaa gatgacgacg acgaagatga cgacgaaggc ggcttccgct gaggcggttt
     9961 ccggctgtcc tgcctgtttt tcaggattca gtgttaacgc ccgcacaccc tttttgtgct
    10021 ccggtggggc gggtttttct aaccatccat gtaacaaaga ttgaaataca agggaaaaaa
    10081 catgacaaga gagtatctgc cgggaactga aattgagatt atccgacagg ttgagcgcgg
    10141 taaaatcaag catatccttt ttgactttga tggaacaatc agcctgctca gagaaggctg
    10201 gcaggggatt atggcgcctg tctgcgtgga aatgatctgc ggcagtacag agcctaccga
    10261 ccagatccgc gaagaagtca atgtcatgat tgacgagaca acgggtattc agacgatttt
    10321 tcagatgcag cgccttcagg aaatggtgcg tgctcacggg cttgtgcccg aagccgaaat
    10381 caagagccct gaagcctata aagaggtgta caacgacaga ctcatggttc cggtgcgaga
    10441 gcggatagcc gccatcaaaa gcggagcgcg tacccgggaa tcctttaccg tcaaagggtc
    10501 gctgcgtttc ctcgagctgc tctcccgact ggatgtgacc atgtatgttt tcagcggaac
    10561 cgaccgggac gatgtgcgca atgaagcatc ctgtctgggc gtagccgatt actttaatga
    10621 aatctgggga gctcttccca gcgtggaaga atattcaaaa gaaaagatca tccgggaaat
    10681 tatcagtcag catcatctgg aaggcccgca ggttcttgcc atcggtgacg gtcccgtaga
    10741 actgcgtaac gttcgggatg ccggcggcat tgcgctgggc atcgcatccg atgaagtcaa
    10801 tggggagggc tgggacgagt ccaagcggga tcgccttctg aaagccggtg cagatatgct
    10861 ggtgcctgat ttcaaagagg cggaacaact ggtggaatat cttttcccct gagcacagat
    10921 gcggcagacg ccgctttgcg ctgcctgttt tacctcttgt gccaaactgg tttatactta
    10981 atccctcaca gatctttgcg actacagaac tggaacattt atcgaggcgg tatgacttct
    11041 caaaacaata ttcaggacgg actttctttt gatgatgtgc ttcttgtgcc tgcacgatct
    11101 gcttacaccc cccgtgatgt gcagatccaa tcacggctga cccgtacact ggggctgaat
    11161 attccgctgc tcagtgccgc catggatacg gtcaccgaat ccaagcttgc catttccatc
    11221 gcccaggaag gggggatggg cattatccac aaaaaccttt ccgctgaaga acaggcggaa
    11281 gaagtggatc gcgtcaagcg ctctcaagcc gggattatta ccgatccttt tacgctgcgt
    11341 gccgatcaga aactcagcga tgccgaagcg cttatgtcgc gctatcatgt gtccggagtg
    11401 cccatcaccg acgagaccgg ttatctggta gggattctga ccaaccggga tctgcgcttt
    11461 gaagaggata tgaacgttgc catttcaacc atcatgacgc cccgcgaacg acttattacc
    11521 atgccgccga atacggacat ggaatcggcg aaaaaactcc ttcacaagca ccgtatcgag
    11581 aaactgccgc ttgtcgatga tggagggcgt ctggtagggc tgctgacgat caaagacatc
    11641 atgaaagcca gggactttcc caatcgcaac gctgacgcca tgggcagact gcgcgtgggt
    11701 gcggcactgg gtgtcggtga tgacgagatg gaacgggcaa aagcgttact tcaggccggt
    11761 gcggatgtac ttgtgcttga ctccgcccac ggtcattcag aactgattat cgaaatggtg
    11821 cggcagatca agcgctcctt cccctcagca cagctcgttg caggcaatat tgttactgcc
    11881 gaagcggcgg aagatcttat tgacgccggg gccgatgctg taaaagtggg aatcgggccg
    11941 ggtgccatct gtaccacccg ggtgattgcc ggagtcggcg ttcctcaatt gaccgcaatc
    12001 ctgaatgtcg cttctgtctg ccgcgaggcg gatattcctg tcatcgccga tggcggcatc
    12061 aagtattccg gcgatattgc caaggcaatt gccgccggtg ctgacagcat tatgatcggc
    12121 agtcttttcg ccggcaccga ggaaagcccg ggcgaaaaag ttctttatga aggcagaacc
    12181 tataaacttg ttcgcggtat gggttcactc aaagccatgc agcgcgggag caaggcgcgc
    12241 tacatgcagt tcgaaactga tgcgtcaaaa cttgtgccgg aaggcatcga agcgcgcgtt
    12301 ccttaccgcg gaaatctagc caattatgtc tttcagctga tgggcggact ccgtgccgcc
    12361 atggggtact gcggctgcaa agacatccct tccatgcagc gcaaagcacg ctttgtacgc
    12421 atgacttccg ccggaatccg ggaaagtcat ccgcatgatg taacgattac cgaagacgct
    12481 cccaactacc agataccttc ctgactttca gggaagaagg acctgtttta tgcaaaacag
    12541 tctgaaacct attgttgttt tggacttcgg caagccggac ggcatgcagc ttgtgaggcg
    12601 tctgcgcgag aaagaagtct atacgctgat ccttccggcc gccgctgacg tacagctgct
    12661 gcagcagatt acgcctgccg gattgatatg cttcggcgcg gataaagcag agcagattcc
    12721 gccgcgtatt gcggcgctgg gactgccttc tttcagcatt gccgcagatg aagagccgac
    12781 gccggaactt tttgcctttg ccgcatcctg ctgcggcgaa tcggttccgt ggaacatgga
    12841 acgctatgcc tctttcatgc tggaggatat caggcagcgc gtcgggtcac ggaaggtact
    12901 ttgcggactg agcggcggcg ttgactcctc cgttgttgcc gcgctgattc atcacgcaat
    12961 cggagatcag ctgacctgtg tgcttgtcga taccgggctg cttcgtaaag gggaagccga
    13021 tcttgtacgt gaagtctttg agggcacatt caaaacgaaa ctgatctgtg tccgggcaga
    13081 agatcgtttt ctctctgcgc tgaaggatgt taccgagcct gaacagaaaa gaaaaatcat
    13141 cggcgccctc tttatcgaag tctttgaaga agaagcgaag aaactgggac atatggactt
    13201 tctggcgcag ggcacgctct atcccgatgt catcgaaagt taccaggcgg cggcaggcgc
    13261 atcggtggca gtcaaaagcc atcacaatgt gggtggtctt cctgagcgca tgcagatggc
    13321 gcttctcgaa ccggtgcgcg atctttttaa ggatgaggtg cgcgccctgg gcaaggtact
    13381 ccagctcccc gatatgctgg tgggaaggca tccttttccc ggtcccgggc tcggtgtacg
    13441 ctgcgtgggc gccatcacta aagaccgtct ggacaccctt cgccaagccg atgccatctt
    13501 tattgaggaa atccgcaatg ccgggctgta tgatcagatc tggcaggcac tcgtctgcct
    13561 gcttccggtg aaaagtgtgg gaattaaaga tggaaaacgt gtctgggaag aagtatgcac
    13621 gctccgggcg attaattctg ttgatgccat gacggcacag cccgcactgc tgcctgcccc
    13681 gctgctcacg aaaacagtgg aacgcatctg cactgaagta ccaggagtca gccgggttct
    13741 tcacgatata agccctaagc cgcctgccac gatcgagtgg gagtaggagc gggccggtcg
    13801 gcaggcatag agcactgttc cagccccaaa agcctgtgca tttctgtgcc tggaaggtga
    13861 acagacaagg gcagtccccc ccttttctct gcgtaaattt tccttcttcc gggattattg
    13921 cggaaaaaaa gccccttttg tctccagcgt caaaaaacaa ctgtcggtaa agttgacaac
    13981 tcctgttttt ttctgataaa ctatgccaac cagttgagcg ggagtaactc agtggtagag
    14041 tgcaaccttg ccaaggttga cgtcgcgggt tcaaatcccg tctcccgctc catttttttt
    14101 tcacccccgg aggggccgtt agctcagtcg gtagagcagg tgactcttaa tcacaaggtc
    14161 actggttcga atccagtacg gcccaccact atggtatgct gtcaggcggg gcgggcgttt
    14221 caatccgtcc gccccgcctg tttgttttta ccatgcccgc ccagagggca attacaggaa
    14281 aatgcctgat tttgatccca tcgggcgagt ggcggaattg gcagacgcgc tggacttagg
    14341 atccagtatc gcaagatgtg cgggttcaag tcccgcctcg cccaccagtg taaacctttt
    14401 ggaagagagt acgatccatg acagaaaaag acgttgatgc aatggaaaca tctgaacagg
    14461 aatccacagc cgcggcagta gccgaaccgg atgaaacggg cgaaactgac gaacgcctgg
    14521 tcgctgacgt ggagcaggac gatttcgact ggaaaaaacc gcctgtcttt gacattgcac
    14581 ataaagaaga ttgtgtatgc gaagttaaag tgacgattcc tgctgaaaat gtcatggctg
    14641 ttctggacaa tgtttataac gaaatgaacg acggcgtgca ggtgcctggt tttcgccggg
    14701 gtaaagcgcc gcgcaaactg cttgagaaaa ggctcggcaa atatgcgcac agttcagcca
    14761 tcgaacagct gaccgatgcc gccgccaaaa ggcttgttct tgagagcgga ctgcgcccgg
    14821 tatctacagc cgaagtaacc ggtctggaag acaaagaaca gctgaatgat caggaagacc
    14881 tcgtttatac gatcactttt gaagtgcccg gcacctgtgc gcttgctgat ctggcagaaa
    14941 tcgagctcga gcgccccgaa tacgaggttt ccgaggaagc actcgaatcc agcattaaca
    15001 atattcgtac ccgtttcggc cgatttgagc cgctcgaaga cggcgttgct gaagagggtg
    15061 atcaggttgt aatcgatttc aaaggcacta tagacggtga ggcctttgaa ggcggcagtg
    15121 ctgaaggcta tccctatatc ctcggaagca agcgctttga tgaaaacatg gaagccgcca
    15181 tggtcgggaa aaaagcgggt gatacagcaa cggcggaagt ggtattcgcc gatgattatg
    15241 ccgccgctga agttgcagga aaaaccgctc agtttgagat cagcatcaac gagatcaagc
    15301 ggcgggtact tcccgagctc aacgacgagt tcgctaaaaa agcaggccat gagtctatgg
    15361 aagtgatgcg cgaaactatg aggaaacggc tggcggaaac agccgacgaa gagatcgggc
    15421 atctccttga agaacaggtg atgcagaagt tggttgatgc cagtaccttc gtcctgccta
    15481 aaggacagat tcaaaaattt atcgacgccg agcgtgcatc tattttagag cgcatgcgcc
    15541 agcagcacat ttccgccgaa gatatggagc gggaagaaga aaatatccgg gaagcctccg
    15601 aaaaacaggg gctcttcagc ctgaaagcca tgtatgtcat tgatgcgctg agcgaacagg
    15661 aaaaaatcga aatagtcgag caggacttcg atgattatgc acgccagttc gtacagggcg
    15721 acaatgccga gcagcttgaa gctttccgcg cttatttcca gagtgatgac atgcgcagct
    15781 ccacggaaca ccgcattctc tacagcaaaa ctctgaaagc cgccattgcc aaatgcaaaa
    15841 tcaaggtcgt atcgcctgaa gagctgagca aaaaagaagc ggaagcggat acggacgatg
    15901 cggcaaaaga ggaaaacaac gatgcctgag ttcccgctgt ctggtttaaa tacgatccgt
    15961 gccgctctgg acatgagcga tcccaagcct gtgtcccctg aagccgtcac gatttaccag
    16021 cagacaagcc gcggcgaaag agcctatgat atcttttcca ggctcctcga agaccgcatc
    16081 atttttctcg gatatcccat tgatgatgca gtcgccaact atatcattgc tcagatgctc
    16141 ttcctggaag cggaagaccc tgataaacct atcaatatct atatcaattc accgggcgga
    16201 agcgttactg cgggacttgc catttatgac accatgcagt atatccgccc ggaaatcacc
    16261 accatttgta tcggacaggc tgccagcatg ggggcggttc tcatggcggc gggaacaaaa
    16321 gggcggcgct tcgctttgcc ttattccagg tttctgctcc atcagatcat gggcggcgtg
    16381 cgcgggcagg caaccgacat cgacattcag gcgcgcgaaa ttgtgcgtat cagtgaaatg
    16441 accgatgaaa ttctggtgcg gcataccgga cagcctctcg aagtaatacg aagagacagc
    16501 gaccgcgatt ttttcatggg agctgaagag gcaaaagaat acggcctcgt cgacgaagtc
    16561 ctgcacagac tgcctacagg cgcctgagag acccgtcgca gcgccgcacg cttccggaaa
    16621 gcactgctgt aaacgtgctc ctgtaaaggc tgtccgccag cagcactata tattgttgac
    16681 aaaaagccgc gaaactgcta taattacact ctatgttgtg gttgttgtct gttcgttgcg
    16741 ttctttaaat gaagcagagg aggcacattt catggcttca cagcaaggct cacgccgcaa
    16801 agggcctaca tgttctttct gcgggaagtc ccacgcagaa gtcaataaac tgattgctgg
    16861 tccggatgtg aacatctgtg acgaatgtgt ttccctgtgc attgatattg tggctgcaga
    16921 tcaggggcgc atcaaaaaac gcaaagcgtc tcatgtgccc acaccccagg aaatcaagga
    16981 ctttctcgat cagtatgtga tcggtcagga tcaggcgaaa cgcattttat cggtcgccgt
    17041 acataaccac tataagcgcc tcgccgctga ttctgaagtg gacggtgtgg aactgcagaa
    17101 aaccaatgtg ctgcttatcg gcccttcagg gtgcggcaaa acgcttcttg ccgagagcct
    17161 ggcacgcatg cttgatgttc ctttcgccat cgttgatgcc accaccctga cagaagcggg
    17221 ctacgtcggt gatgacgttg aaaatattgt gcttaagttg cttcagtctg ccgattttga
    17281 tcatgaacgc gccgcgaccg ggattattta tatcgatgaa atcgacaaaa ttgcgcgtaa
    17341 aagcgatagc ccttccatca cccgtgatgt gtccggtgaa ggggtgcagc aggcactgct
    17401 gaagatcatt gaagggactg ttgccaacgt gcctccccag ggcgggcgca agcatcctca
    17461 gcaggaatgc gttcaggtcg acacacggga tattcttttt atctgcgggg gcgcctttaa
    17521 cgggctcgag cagatcatac gaaagcgtac cggcaataat gctatcggat ttaacgccga
    17581 aattaagtct aaagaccgaa aacggcttgg cgatatcctc gcacaggcac ggccggaaga
    17641 cctaatctct ttcggtatga tccccgagtt ttcgggtcgg attcccgtga ttgctacttt
    17701 gtacgatctt acctccgatg atctggaaaa aattctgact gaaccgaaaa acgcacttat
    17761 ccggcagtat cagaaaatgt ttcagatgga taacgttgaa ctgcagtttc agcctgagac
    17821 catcagggca gttgcggaaa aagcgatttc cctcgaaaca ggtgcgcggg gcttgcgtgc
    17881 catcctcgaa gaggcgatgc ttgatcttat gtttgagatt cccagccgca aagatattaa
    17941 agaagtcatt atgactcccg gtgccatact caatcgggaa gaacctattg tggtttacga
    18001 gcaggaaaaa cggaaaagcg cgccgaaaaa ccagaacagc agctccgcat ccgcatgatt
    18061 tcctgattcc gcccgacagc tccgccgccc gtgcgcctgc cttttcagtc agctgtttcc
    18121 tgataagggt acaggtatat ctctgcggcc agacaccgga gaagcgggaa gccacctttt
    18181 tcttctgctt ttgcgctgag aaaagacttt caggagcgtt ccaactggaa gcgggcaaag
    18241 agcggggcat gatcactgag gggcgttttc ccatcggcat aatagaaatc ggggtcgtcc
    18301 tgctgccagt ggagcacctt catggcgcat ccctttccgg ggcgaaagag gatgcggtcg
    18361 atttctttgc tgacaggtcc gttgaattca tcggatgcat cacgcagatc cagggtctct
    18421 ttaatccagt cgaagacata gaccttggga ttgtctttgt gcttttcctt gtcttccggt
    18481 ccgcggctgg gtctcatatt aaagtcaccc agaataatga cgctgttttc cggaggggat
    18541 tgagcattga caaagtcgac gatttcttca ccctgtcctt tgcgtgccgc cagcgcttga
    18601 gggcttccgc ctgcttcgat atgggtagta taaacgtcga gggtcatagc tcccgcctga
    18661 taccgtccga ggagtactcc tttggacgca gggcgattct gaaattcgcc gcaggtgtcg
    18721 aaattaatat tgtccgtact aataagcggg agtgatgaaa gaagggataa tccgcttccc
    18781 actaatttga gagggtgctt cagggtgccg tgaaaaatct tggaggagtg ttccgctgct
    18841 gcccaggcac ggcggtagtc cttgaagcat tcctgcaagg cacagatatc gaagcggttg
    18901 aggagcgggg agatgtgggc gaatttgttt tttgtttcat cgaagagagg ccgtgcctgc
    18961 acgttatagg tgcagatagt aaagacgacc tcctcggcgt cactgggaaa aggggagcgg
    19021 gtctggtcgg tcgtttccac aacgactttt ccccagcgca tcgccagtac tgcagcggct
    19081 gtgatgatga acaacagcag cgtaattccg atcctgcgaa acaaagtact ccgggagaga
    19141 ttggatttca ttattcattt ccgtagctca gacgtttccg cccggcagtc agtcctgaaa
    19201 taatgactct cccggagttt gaccggtttc aactgacttt cacattaaaa tttttcccaa
    19261 tggatcactt cttccaaagg acgcttttcg ggagtggttt catcattgga atagcccagc
    19321 gcaataatac tcaccagttt gatttcagca ggggcgccca gaagctgttt catggcggcg
    19381 ccatattctt ttttctcgcc gtcgatccag gcactggcaa taccgagggc tgttgccgcc
    19441 acaagcatat tctgtgttgc ggcgcatccg tcttcgaggt aaaaggggct gtccttgcag
    19501 agcacagcga tggcggcgga agcgctggca aggtgttttc cgtattccgc caggtctgcc
    19561 attttcttca gcatgtctct gttggtgatg atgacaaact cccagggctg gatattgatt
    19621 gccgtggcgg caacacgtcc ggcatcagca atggtctcca gtgtttcttt ggaaatggat
    19681 tttccgggtt cgaattcccg aacggtacgc cgaaggcgca tggctgtgag ggcatccatc
    19741 atgatacatg tcctttctgt ttccggttcg caaagaacct gtctattgga tgatcatcag
    19801 gacacagcgg actcaggggc tgacctcctt tgcctcgcct ttgtccagat aaagaatcag
    19861 atctcttccc gacgcactgt gacgggagat gctcagcacc cggtgatcag gatcaccgac
    19921 ctgtgtatcc acatagtttt ccgctgattt ctggtcgtca aaaatttcta tgttgatgtt
    19981 ttcatagtca tcataccggg cgcggagctg cctgcccagt tcctccacat cggcacgcct
    20041 gcttccggga ggcacgacgc cgaccagtcc ttttagagtg gagacagcat gatccagttc
    20101 ctccaccatc tccgggctgc ggccccattc gtgcaggatg gtaagggaag ctggctctgc
    20161 aggggcgccg ctgtccgctt cttccggcac ctgtcctccg tcatcgggca ggaaggaggc
    20221 accgctctgc tccggtgtta caggcgtgtc tttttcctcc atgcccggta ccgcctgaag
    20281 aagtccgcgg ataataccga tacccggccg ggggatgtgc gtggtgtccg ggatgccgaa
    20341 ttctttctgg atgaggtaaa ggcgctgtac ttcctcgcgg agtacgcggt tttcttcctg
    20401 catatccgcc atgatgtggt tgatcatgag atccagggtg gactggagca tgcgcagttc
    20461 atcccggacg acctgaatct ccagaaggac tttatcgaga gggtctgtcg gcgctttcgc
    20521 tgcgtcctcg cctgctgcat caggcaccag atcgggcttt gccacctgtt ccggcgagaa
    20581 gacccgcact tccactttag agggaggaaa cgtgtcactg cttttctttg tttctgccgc
    20641 tgttccggaa aacgccgcaa gcagcatcag agtcagcagg agaaacatcg gatttaaggc
    20701 attcttaacc atcacttcgg aaacctcttt tctgagtaac atacaggaca gggtaaatta
    20761 atccgctgag cttttttttg ggaaataccg cggatgcgtc cgggcgaaaa acagttacag
    20821 cagccgggga accaactcga gaaatcggtc ccaggaaggc agcagataat cagcacgggt
    20881 gcttccggca taacgggcat tgacagctgt gatcagagct gcttttgcgt gcacctgatg
    20941 ggcgccggtg atatcggtgg gctcgagatc gcctatatga agcagctcgt gaggcgcgat
    21001 gccgagccga tcggcaatgg atacatacat gcgccgctgg ggctttgcgg ctcctgtctc
    21061 atcggaaaaa ttcagggcgc tgaaatagga aagaaaaccg gcattatcca gcaggatgcg
    21121 caggtttctg cccggactga agcccgtgtc ggagataagg gcgacgggta cccgctctga
    21181 tgcgacacga acggcatcaa gcgcccggtc gatgggcacg ggcggatact tcagaatggc
    21241 gtcaccgaag cattgggcaa gttgtttcag ggttgtttca tcggcatgta cgttcaattg
    21301 ccggcagcag agctgtgccg cgtcgtgggg agtgagtgtg tgctgcgtgt ccagatgatt
    21361 gcgcataaaa aatgccatgg tgctgcgcag tgctctgtcg gcatcctgtt ccggcgcgcc
    21421 tgttgcttcc acgagcccgc gcaggcgcag cccgtgccgc tcctctcctt gtgcatcacg
    21481 gaagagggtt ccccagaaat caaaagttac ggcgcgtata tacatgattg ctctgaattt
    21541 ggtatggtgt attcgggctg tccagtgctg ggcggatgca cccggcaccg acagaagact
    21601 ataatagcat cattgctgtg accgacaaaa aaaggaaaag acacatgccc gcttttgcac
    21661 tgcgcccagg cagtattgcc acactcatct gccttcttgg gtttatttcg ccgactcttt
    21721 catccaccgc cgattacggt gctgctgtgg agacagtgat agagcatgac gacggtgaat
    21781 ggctctggtt tcatccccgt gcggcggcgg cacccggttt tggcagagat ggtgcccctg
    21841 ccgtggtcat gacccttcag aaacatcttc agatttcaga ctattactca ggtctttacg
    21901 cgctgtacag tgatgatctg ggcggcagct ggacaggtcc ggtggaaata ccggagctgg
    21961 catggcgtga cggcccgggc ggcaccacga ttgcggtctg tgatgtgacc ccgggctggc
    22021 atgcgccgac agggaaagtg ctggcgctgg gtgcgcagat ctgttacagt cctgacggac
    22081 gacaggttgc gcaggaggag cgcttcgatc agactgccta tgccgtctat gatccggcga
    22141 aaaaaagctg gacttcctgg aaaattctgg aaatgccgga tgatcctaag tttcactgtt
    22201 cacgcaatgc ctgttcccag tggctgactg aagataacgg agacctgctg gtgccacttt
    22261 attatggagc ggcggggcag tcggattaca gcgtgaccgt cgcccgatgc cgttttgacg
    22321 gtgaaacact gcgttacctc tgtcatggca cagaaatgac agtgcccggc gggcggggtc
    22381 tttgcgagcc ctcactgatc cgttttcaga accgttatta tctgacgctg cgcaatgatg
    22441 cccggggcta tgttgcagtg agcgatgacg ggcttcattt tgaggtgccg aagatatggc
    22501 gttttgatga tggtgccgag ctgggttcgt acaatacaca gcagcactgg cttgcctccg
    22561 gcgatacgct ctatctggtc tatacacggc ggggagcgga taacgatcat atcatccggc
    22621 accgggcgcc gttgtttatg gcacaggtgg acacggaacg gtgttgtgtg atccgtcaaa
    22681 gcgagcgtgt gctgattccg gagcggggcg cagccatggg caacttcggc gcatccgcca
    22741 tgacagaatc ggaggcgtgg gtgacggtgg gcgaaggggt gtggaacgat gacatgcgga
    22801 aaagaggggc aaaaggagca ctgcttattg cccggatcga ctggaacagc agcagggaga
    22861 atcagcagat gaaagcagcg gaagtgctgg cggcatctct tgagccgtgc gctcagaaac
    22921 cttttatcac tacccttttc agcaaagagc cgctggtctg ctgcggcaca tcctggtatt
    22981 gtgctgcttc gcccggcttg ccggagaaac tggtcattat gccggtgctt agacctgaag
    23041 ggataatccg gatggatacc ggggcatcac tggagtatca gtatgaagag gcggaaggga
    23101 agggcggcat ggtgactgta ccgcttaaga gctgcaggga tctgcagcag gggatctgca
    23161 ttcagtgggg cgaaaatcgc tggcagaaaa acatggatca gttcgcagag gataatctga
    23221 acagaacacc tatgcggggc ggtatgctga tgacaggcag ttccacggca cgcatgtggg
    23281 atgtggagcg ctgtttcccg ggcagacagg tgctcaaccg gggtttcggc ggatcctgct
    23341 accgcgatct tttccgcttt gccgacgagg tgattgctgg gcatcggccg gataccgtag
    23401 ttatttatga cggcgataac gacattgccg gggggcttac gcctgtccag acagcagcag
    23461 actgccgtgc atcggtggag cgtatccggg cactgcttcc ggacagccgg attatcgtgc
    23521 ttgcaaccaa accaagccgc tcccggtgga acctgcacga agccatgttc gcttcaaatg
    23581 ctgccatcgc acagcagctg agtgctctgc caaatgtgca gttcctcgat cttattccgc
    23641 tgattatgga cggtgaaggc atgcctgatc cggtgtgctt caaggaagat gcgctccatc
    23701 tgaacgatga ggggtaccgc cgctggagtg ctgctctaga gaagtatctg cccgcagaaa
    23761 aaaaagagta aggcagggac aggacagaaa agagtgctat gaatatgctt cgccgtcttc
    23821 tgatcaacag cctgcttgtg ctgttttcgc ttaccctgac cgtgctagcc gtcctcggcg
    23881 cagaccggct gcttggcatt ctgaaagcgc cgcctgatct gcccaagtgg gtggagctga
    23941 actttccggc gggagatgaa cagcactaca gcacctcaga attcagttat gatgtgtata
    24001 tcaacagcct cggactccgc gaccgggaat tgcccaggga acgggggaaa gcctttcgga
    24061 ttgccgctat cggcgattcc tatacctacg gctggggcgt gaatatagaa gattgctgga
    24121 cgagtctgct gaacgagcat tttcgggaag caggggagac tgtggaagtg atcaacctcg
    24181 gcaagcccgg gcagggaccg ccggagtatg ccgcactggc ggaaaaagcc attccgctgc
    24241 tgcgccccga tctggtgctg gtttgcatgc ttcagggcaa tgatatcagg ggagtgggac
    24301 cggagcccga tgcgcttccg gcgccggatt tttatgaaag ggtacgtcgc tgcttcccca
    24361 atacgacacg gctggtgcgt gatttgcagc gggagcggga gtatacgggc cgcagccacc
    24421 ttgccatgcc tcccggacgg agcacagcgg aagataaccg ccgctggatg gcaaatacgg
    24481 cgcatgaaat ttatgaaaaa tacagcgatg aagaaaaagc gcgcttcgat tcttttgaag
    24541 agcccgtgcg cactgctttc atgcagggcg atcttaatgc ttatatggtc aatctgtccg
    24601 tgctgaatcc ggatttctat attatgacca tggatctgga ggacgcctgg actgaaacct
    24661 gtatcagccg ggctgcacac gcattatcac gcatacggaa agttgccgaa gactacggtg
    24721 cccgtacagt ggtcgttgcc atgcccgaag gaccttatgt aaatcgtgcg gcactggaag
    24781 gcatgcggcg tatcgggcat aacatgcctg agtggcttct gcagacggat gccatggaca
    24841 aaacgattga gactgcagca acacgggcag ggctgccctg ttacaaagtg accgatgcgt
    24901 tccgtaaaga aatagatcgg accgatcttt attttccgct ggacggacac cccaccgccg
    24961 ccgggcatct tctttttaaa gaagcctttc ttcctgccct ggaaaaactt attgccggac
    25021 agaaaaaagg cggacaataa agcatagcct gcggcgcggg gcagaaacgg ctgtgtctga
    25081 ctccggaaac tgagcgcaaa gtgcggcgag aacgattcca ggttctgcag gatttctccc
    25141 ggcacagtca gagcttcctg atgcgccaga taacgacagg gggcggacaa caaaaaaata
    25201 ccggtactgc ctgttgcggc agtaccggta ttgcattcag aaccggaaat aggggatcag
    25261 attttcttgc gcagggcgct gcctgcaaga agtgtgctca ggcacagacc cagaagcagc
    25321 cagtcaccca gcaaggcttt tgtcgatgtc cgcagatctt tggatttgca gccgcagcct
    25381 gatccggtgt tgctgtcttc accttcgatc acagattcgc cttcggtcac gggctcccct
    25441 tcgattacgg gttcaccttc gattacgggt tcaccttcga ccggcgtctc accttcaacc
    25501 ggcgtctcac cttcgaccgg agtttcgcct tcgacaggag tttcgccttc gacgggcgtc
    25561 tcaccttcga ccggtgtctc accttcgacc ggcgtctcac cttcgaccgg agtttcgcct
    25621 tcgacaggag tttcgccttc gac
//
LOCUS       DAOPWB010000034        24870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_1972_length_24870_cov_5.117792, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000034 DAOPWB010000000
VERSION     DAOPWB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 24870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24870
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1972_length_24870_cov_5.117792"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PKN92_04895"
     CDS             <1..1015
                     /locus_tag="PKN92_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48160.1"
                     /translation="NFSPFINTRPGAPASERYLAVAGTEESGLSSFSSEDGIHWTLKD
                     EKIITGGAFDSQNLAFWSEAEQCYLCYFRTWTKGNYAGYRWISRASSPDFVHWSPPEE
                     MDAGNAPPEHIYTNQTLPYFRAPHLYIALAARFMPGRHIMSEQEAAVLGVEKGYYKDC
                     SDSVLVTSRGGTRYDRCFLEGYVRPGIGLENWTSRTNYPAWGILPTGETELSLYIQHN
                     YGQPTARIDRYTLRTDGFASLRAGCTRGLLTTIPFTFTGKDLHINFSTSAAGSVRAAL
                     TDEAGTAYPGFSLEESNEIFGNSIDRIVRWQDENSAGILAGKTVRLVIQLADADLYAF
                     QFK"
     gene            1117..4041
                     /locus_tag="PKN92_04900"
     CDS             1117..4041
                     /locus_tag="PKN92_04900"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNZ48161.1"
                     /translation="MKRMLSFSLTTIALLVLLSGCPWFKPPVPPVDPVEGEEVIEGEL
                     PAEGESPEEGEAPLFTSADPAQRGVYDETLDGVPTPDAGEENEGGKESRELVEPDVIR
                     RAGNLLYILNQHRGLIIADLDGQRTLSQTPTFGYPRDLYLVGNKAYVLVSYAQDVRLD
                     EKGRYQVSYGSRLYVFDVTDPEAVVSLGSSRFEGDLIDSRLVGSILYAVCSNYTWYYP
                     EETDEPIETASVEKSYGDTWAISLDTARPGAMSQVAKVKFQGYGNLIQATSEAIFCAT
                     SDWYSDSTAITYVDISDPRGAIAVRGTAVVPGHLADRFKMDAWEDVLRVVSNTGWNDR
                     KTLITTWDLKDPDTFAQLGSTELESAAGDTTYATRFDGARAYIVTYLTVDPLYVVDLS
                     DPANPQVLGELKIPGWSTHIEPRGDRLIALGVDDEGGGRRVMVSLFDVSDPGAPQRLD
                     YTSFGDNWSWSTAYEDVKAFTVLDNAILVPFSGWNYGSGGYDRLQFISYNRDRLQAQG
                     YVDLRGSALRALQYGGYHFAVTQEQLAVIDASDPNTPRITNSITLAENIADVVPLKDG
                     WALEIICRYDSADSSVRAVNAAGETGQEVLLKTSSITESFLWNDTAVLVAAGYDYYPE
                     YNSFYRVYLVNVSDPLSPVLEKEILVKLQPWYYGWWMGYDMVASGKQARPGYYPYYYY
                     YDPSPTAFLAGDYLVMRGSGDRFDRYLGASSAYQGLAVLNLNDPDNMTYLGLGDPAPE
                     RVSGDGKQIYLTYRKTLATSSGNAYRCAYYLSTLDPATLDESARVNVPGIFQHRVPGT
                     DILLLKDTQYLRDGNLKELLRSGILSENKFSLQDSVNLPSYGTQLALSDNFAGYVGYI
                     YSENSDVPRDTLLSSGNDKAAEEARGYMLGGIFVNAKGGFKSHERKNVGDVWCNLLGV
                     RKGSVFLSVSGAAASQWNFNEHPPEHVTLFPVMGYPSKLRVGKDTAYLPLGYGGLASF
                     PL"
     gene            complement(4083..5243)
                     /gene="bshA"
                     /locus_tag="PKN92_04905"
     CDS             complement(4083..5243)
                     /gene="bshA"
                     /locus_tag="PKN92_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNZ48162.1"
                     /translation="MKIGISCHATTGGSGIVATELGLALARRGHEIHFVTMEPPFRLP
                     HYEPNIFAHSVDTINYPLFKLPPYSIALSSKIAEVAQEYDIQLWHAHYAIPNAVAALL
                     GRSMLPAEKRFCLITTLHGTDITLVGSHPSFYRATQYAMESSCAVTAVSDWLSRETER
                     EFDLKEPVRTIYNFLDPTRFHTRKINRPTLARGDEKIIMHLSNFRPVKRVTDVIRVFK
                     RILDDVSARLIMVGEGPERLSAAGVARQLGVEDRISYLGNCANVEDLLPCADLIIQPS
                     EHESFGLVPLEAMASEVPVIATNSGGVTEVVVHGKTGYLCEVGDIESMAEYARAILTQ
                     PELRTALGQAGRKRALEKFSVDLIVDQYEHLYEEVRARRADVMEKKHSPEPW"
     gene            complement(5286..6125)
                     /locus_tag="PKN92_04910"
     CDS             complement(5286..6125)
                     /locus_tag="PKN92_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006510516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HNZ48163.1"
                     /translation="MFFYSARRKLFRLAASLITWSFFGGCCAPLPPSDYAPPEEIIYY
                     ECEIVTVYPHDSGAFTQGLVFENGCLYEGTGMHGKSSIRQVALESGELIRQHLLSSSY
                     FGEGIAIQGKRLFQLTWRSNMGFVYDLDSFEQTGDFGYLGEGWGLTHDGKRLIMSDGT
                     SRLRFLDMNSFEEKGSVHVRYGSSPIQKLNELEYINGLVYANIWYDHRIAIICPESGY
                     VTGWIDCSSLVKRPEIARADVLNGIAWDRDGERLFVTGKYWPELFEIRLIEKARAPYP
                     AVE"
     gene            complement(6286..7368)
                     /locus_tag="PKN92_04915"
     CDS             complement(6286..7368)
                     /locus_tag="PKN92_04915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ48164.1"
                     /translation="MKKIVALSVLFCLVVFSAGAQVPDLAKLDIVERSVPAGPVALVN
                     GVAVEGRYFLEEYRRHLRNVMSLVADQNLTDEFRVHAGLTTLGELLREEILVQEASRR
                     KIQVTSKDVETEYQEKLRAFSQYIEETSGEKVGEAQVLERARQSREEALDSIRRQLLA
                     EKMAEELAKEKKLSVTDGEVKKFYDENPNFFQMPGSMHLNHILVVPKPSAAKADDKAW
                     TKATEQIRHAKSRIEAGEQFAAVARSVSEAPDAEKGGDMGMLPVNLLPPFYVEAGQAL
                     KPKELSEPIRSQHGMHLIQLVDTESQHAISFEEARENIRAQLKASKMEEIVLEFCEPV
                     INDTSKTQIFLQLDRNLAVTNKEATK"
     gene            complement(7436..8482)
                     /locus_tag="PKN92_04920"
     CDS             complement(7436..8482)
                     /locus_tag="PKN92_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ48165.1"
                     /translation="MSENKRSLFMQALRGEKTPRVPVWFMRQAGRCDPAYLALREKSG
                     LPLEHLFRSPDLAARISLLPLAWGVDAIIFFQDILTILEGMGAFFRFAPGPVLANPER
                     FLTEPGALHRCDVREAMPFVGESLTLIRQSAGQEYPLLGFAGAPLTLLAFLSEGKSPS
                     SELSVLRSMMRNCPERVDALLDLLTVMTVDYLKYQFSGGADAVQLFESCAHLFSRDEY
                     LRFALPSQQRIFAALKGFGPTILFSRLDEAGVQLEDLGAAGADVLSLPSCFSITAARA
                     QLGDSLVVQGNVDNVLVADGSKDAIARATEECLAEGQSRGHILNLGHGVLPRTPLKNL
                     VHILETARGYEVDG"
     gene            complement(8550..9002)
                     /locus_tag="PKN92_04925"
     CDS             complement(8550..9002)
                     /locus_tag="PKN92_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48166.1"
                     /translation="MADKYICTKCGRKYVDWGAKKLDFKCPTEGCAENLLVLLGSPEA
                     SLLEISAKSKATKRRQTLVPRADVDYEEEDLGEEYEEDEEELEIDDEEAEDRKPVVLS
                     DGGDDKDGDTDDGLTVIADEDEEEEDEEEEDLSPDLDEEEVDLDFQDE"
     gene            complement(9237..11912)
                     /gene="secD"
                     /locus_tag="PKN92_04930"
     CDS             complement(9237..11912)
                     /gene="secD"
                     /locus_tag="PKN92_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNZ48167.1"
                     /translation="MSKSIYRSLLIGASLVLAFVYVYPTVGWMLLSDESRAARLEKWQ
                     KEDDDLARRQTGYFEELWVGARRWFECDRNKVINLGLDLQGGIHMVIGFDIKDLPQEM
                     LDEYRNERRYSDENIEKEVQQIVLQQITRRINDFEAKEPIIQTLGTNQIQVQLPGEKD
                     VQRAKNLITKTAQMNFHIVAGPDESRPVFEKIRDTFPNEFIPFVKTTSMRPDVLTVSV
                     ENYERVRRVLVRAEESGVIPEDKIIAFSQQPKPYARDQEYHLYVLARKPIASGEGLTS
                     AAAIQDQSNMPYWQILFSFGASAAVNFADATGANIGKPMAIVLDGVVVSAPTIRDRIA
                     GGRGQISGNFEAQEAVDLAIALNSGSMVVPVREEFTRTVSASLGKDTVRSGVYASLAG
                     VTLVALFMIAYYKGAGLISCICLALNALMIIAAMAYFGMTLTLPGIAGLILTMGMAVD
                     GNVLIFERIREELRLGHTVLSSVESGFRRATVTIVDANLTTLIAAAVLMQFGTGPIEG
                     FAVTLSIGIVTSVFTVLVIGRALFDFFLAKGVMKKVSMLSIVPENPRVPFLQLRNFTF
                     LFSGAVIVVGMTCFAIRGMDNFGVDFQPGTNLMLHVENTDSVPVGDVRAALSASGFTN
                     PVVQESSGDSGEGNANVFIVRVGDITRTAEGAEAGSEQLQTVAERIQQALAPLSAAGT
                     AEGVVVEDEQTVGPSVGAQLRWDALNAIFWALLFIVIYLWVRFEFRFALGAVAALAHD
                     LIVAVGVFSLLGGEISMNVIAALLTILGYSINDTIVVFDRVREDIQAQRGKGYRFIDI
                     LNGAINSTLSRTLLTSITTLFVVVVLAVFGGEAIRDFAIVLLLGIVFGTYSSIFVASA
                     LVYLMEKRPAEGGSTQSRKSGKAEERAPRRSSAKA"
     gene            complement(12087..12419)
                     /gene="yajC"
                     /locus_tag="PKN92_04935"
     CDS             complement(12087..12419)
                     /gene="yajC"
                     /locus_tag="PKN92_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNZ48168.1"
                     /translation="MWHLIVAGAEGAAQGAGGAPVNPLGSMLPMIVIFIGIMYFLMIR
                     PQQKRDRERREMLGSLAKGDNVITTGGVCGSVVGLSDDRVVLRVDDNVTMEFVRGAIA
                     QVLPKETK"
     gene            complement(12521..13636)
                     /gene="tgt"
                     /locus_tag="PKN92_04940"
     CDS             complement(12521..13636)
                     /gene="tgt"
                     /locus_tag="PKN92_04940"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNZ48169.1"
                     /translation="MKFRVTHQDPHSSARAGVLELPHGTVETPVFMPVGTQASVKSLS
                     NNELEALGAQIILGNTFHLYLRPGVDVLDYFRGIHGFMGWNRPVLTDSGGFQVFSLSK
                     LNKVTEEGVAFKSPVDGSAHFFSPESAIDIQRSIGADIMMCFDECTEYPAAYDRAAFS
                     VGLTSRWARRCRDRWLEGDTEKQALFGIVQGSVYESLRRESAEALQELDFPGYAIGGV
                     SVGESKEEMQAATGWATAFLPADKPRYLMGVGAPEDLLAAIEKGVDMFDCVMPTRNAR
                     HGNLFTRYGRINIKNARFAKDGLPVDPDCGCPACRNHSRAYLHHLFRVGELLSQRLNT
                     LHNLYFMIQLAIRARRALCSGTYTGFKTAFLQDYHQK"
     gene            complement(13671..14261)
                     /locus_tag="PKN92_04945"
     CDS             complement(13671..14261)
                     /locus_tag="PKN92_04945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ48170.1"
                     /translation="MPLCHKTKGFDRFWTPFLCLNRLLPAFVLVLLLSASGCARSARR
                     IQVLQPPPASGDSGYMAQDYRKAHLIWPLRHHQPRITSPFGEARSQGRWHKGVDLAAS
                     PGEGVFAVSSGIAVYSGVMGAYGNLIILRHNSHLETAYAHLQSRYVECNEPVRQGQQI
                     GTVGNTGNSTGPHLHFEVRKNGQPVDPLPLLPGRRD"
     gene            14407..15219
                     /locus_tag="PKN92_04950"
     CDS             14407..15219
                     /locus_tag="PKN92_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNZ48171.1"
                     /translation="MYRSKINSALIVPVLLCFALLLTACSSQTGESVHIVGSSSVQPF
                     VELLAESYLKNNPEENVDVQGGGSTAGLQAVRTGLANIGTSSRMLTAAESETFDSAVV
                     ALDGLAVVVNPANPLSKITSAELRDVFSGKIANWEVLGGDDRPIRPITREAGSGSRDN
                     FASLVMEGNPISRRAITQESNGAVKELVKGDPAAIGYISLSMLSHEVKALLVDEEAPT
                     IENIQKGHYALTRPFLFVTKGDMSPEARRFFEYVLSDEGQAILEEEGLVRKP"
     gene            15216..16076
                     /gene="pstC"
                     /locus_tag="PKN92_04955"
     CDS             15216..16076
                     /gene="pstC"
                     /locus_tag="PKN92_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNZ48172.1"
                     /translation="MRQEKLIALILQAVALSALAGLLLITVFIFKEGLPLILRTGLKD
                     FFFSSDWEPDAGHFGIMSMIIGSLAVTLGAMLIGGTLGLGMAIVLTQFCPPKLASFLK
                     PTIELLAGIPSVVYGFMGVVTLVPLIRSYLGGPGLSVLAASIVLGIMVLPTVTSISID
                     ALKAVPRTYWEGSVALGATRWQTTHMLMLKAARSGIVAAIILGMGRALGETMAVIMVA
                     GNALEIPGSVLDPVRTLTSNIALEMGYASGEHREALFAAGVTLFAVIMIFNTIALTFA
                     RRTIRQGRGQ"
     gene            16073..16912
                     /gene="pstA"
                     /locus_tag="PKN92_04960"
     CDS             16073..16912
                     /gene="pstA"
                     /locus_tag="PKN92_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNZ48173.1"
                     /translation="MKRISPAITQLIAVGCLWTFTLLTLSILLFIIAYILIQGLPWLS
                     WEFLSQAPESMGRRGGIFPMIVGTFMVAGLAVLIAAPIGIATAVYLTEYTREGKFTFI
                     VRFGADCLAGIPSIIYGLFGFVFFAIALGMGLSVISGALTLTLMILPTIVRTAEEAIR
                     AVPPAYREASYGLGSTRWQMVTRVVLPLALPGICTGIVLSLGRSISETAAVMLTAGSA
                     LRMPHSLSDSTRTLALHFYILSREGLSMDKAYATASVLILLILALNLFAYWLMRQVTR
                     RNG"
     gene            16909..17682
                     /locus_tag="PKN92_04965"
     CDS             16909..17682
                     /locus_tag="PKN92_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNZ48174.1"
                     /translation="MMPPEQNSPVIEVQNLTVTSYDRTILDGLSLPVYKNERLAIIGP
                     SNSGKTTFLRCLNRLNDLQHSFKVSGTILLNGSNIFDPGADTAQLRRKVGMVYAVPVP
                     LPWSIYENLVYGPRLAGVRDKNRLDELVETSLQSAFLWGEVKDRLHMPAKNLSGGQQQ
                     RLCLARVLALEPEVLILDEPCSALDPISTAKIEDALFELKSRLAIVLVTNNTKQAARA
                     SDRTAFFLMGKLIEMKETGRLFTNPSDKRTNDYLVGQFG"
     gene            17690..18445
                     /gene="pstB"
                     /locus_tag="PKN92_04970"
     CDS             17690..18445
                     /gene="pstB"
                     /locus_tag="PKN92_04970"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNZ48175.1"
                     /translation="MDAKIETKNLNLYYGDYHSLKGVNIAIPPRSITAIIGPSGCGKS
                     TLLRCFNRMNELIPGVIIEGEILLDGENIHQQGTDLIRLRRRVGMVFQRPNPFPLSIA
                     DNITYGLRVHGTPPGQKHSDIVENSLRATLLWEEVKDKLHSPALALSPEQQQRLCISR
                     VLAIEPEILLMDEPCSALDPIATDHIEDLMRDLSESYTIIIVTHNMQQASRVSSWSAF
                     MLMGELIEFGPTVRIFNNPRDKRTEAYISGRYG"
     gene            18477..19175
                     /gene="phoU"
                     /locus_tag="PKN92_04975"
     CDS             18477..19175
                     /gene="phoU"
                     /locus_tag="PKN92_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNZ48176.1"
                     /translation="MTRHFQHEIEKLKVRILALAALVEKSVRNAVTALAERDTEMAQV
                     VIGEDREIDLVEVDIEEECQKILALHQPVAHDLRFIIAVLKINAELERIGDAAVSIAE
                     QICYWVNEGHTDVSYDFESMAKKAQAMLHNSLDALVNFNAQTAFEVIAEDDKVDEIYG
                     DVHRFVRRGVQSAPHQSGGFIHLLAISRHIERIADHASNIAEVVIYLVEGRIIRHRAK
                     DAMKQDTFGNGSQS"
     gene            19601..20551
                     /locus_tag="PKN92_04980"
     CDS             19601..20551
                     /locus_tag="PKN92_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48177.1"
                     /translation="MTEKQKAVHTIPFKQWLKEQIPAGMSDIQLPYIEEHLQGKTFTR
                     SYSRKEGEEIRYVYDMTPVGYVRAWVREVHKDNKRLLEYGLQVCDDFEKGLLQVYDLP
                     GKLRGYIEDYMEEVCIEDSPLDNPVWYMSSLLSYSLRFKVAAAVAEDGIKEDVTPTEF
                     CLWARAMNLYINASGAALIETREAILQVIHGQKKNAERQQPPSLNLAKKAGATRWEEL
                     AVRLKGDAIEYRHIDGDTFKRIKAKGIFSKLNSTQYEMLINLARGEGVHTDPRGVGLD
                     TTLSRLNDRLKEAFGLSENPFFRAGNAFLSRIGKLWLHRT"
     gene            21300..23711
                     /locus_tag="PKN92_04985"
     CDS             21300..23711
                     /locus_tag="PKN92_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNZ48178.1"
                     /translation="MSIAKEKSPSAATPGLHKNVAPPLTEPPSSLPEHTSCFNYGVCV
                     KGRYDGSRELVQDWKDTFTKYLACEEEETEAYLTAFSYGDDFLPHYKQYRSNKGFTGS
                     AFGHFLCFDFDCAEDPSDAIAQAQHFLNHCATNYGADMDAVLAGFSGGKGAHIYFPVP
                     SCAVPSVDFGNICLAIAKRFKDPYNCIDLKIYDHQRIFRLPNTRHPKSGKLKVMYRAS
                     DFVELDPLYILNKDTAQNADFLFSDDAGEWLAPVWKAAVNQIEKEAAPLPSRAEGFRD
                     LSGDTVDFIKHGAKEGERNTSLFKAACNLYEMNMTQPAVEKLLAPAARDSGLSEAEIQ
                     KTLQSASKHAESARGRFRLHEACRSSKKFYDLVCDILAGADFPPEGDWGELERHYGEH
                     DFPPVGALDSAQFSEAEVRYLAEQWLETWKAQATQEAKKTLDGAGALIERLQVIEQAG
                     TDPGLYQRASAAPPQGNPIFRNGLLPGSIGAIFGSDGIGKGFIILDLMVGYARGNPLG
                     ITSIGAIPGKRLSTTYVSYEESISDLKTRLDKICGEEVDYGKLEEEGMLRFSTIPKEP
                     LFRYDMKGSLLSTAFMKTLEKAIIRDDVDLMILDPLASAGGLRDENNNAEMNLIATRL
                     RTLADKTGCSILLVHHTTKEGRASLDPSAGRGASSLQGAMRWIVNFRRNADDGQIIEA
                     AVTKNTHGGLCAFSLGRITSGVIRELSSTEIQKRLQAIEITNRLLLQEHISFLVEHIA
                     KHDGEIKKSGVANGRGRGAPELIKALNCLGKKIRAREAVEIIDMAIEAEKLREDEDGH
                     LWIQA"
     gene            24254..>24870
                     /locus_tag="PKN92_04990"
     CDS             24254..>24870
                     /locus_tag="PKN92_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48179.1"
                     /translation="MATVFKKTYTAPIPENAETFTRKGTEYARYKGRDGKSRVERLTS
                     DGARLLLETGTWYARISLSDGGSLTVNTKCKDKATAEQFAANLQTEEDKIRAGVFTKK
                     EVKAARHGQDALQKSIDAYLGSMTARNKATKTVKEFRRYLESVCKALGWKAVRDVDKV
                     ALDAYLEEERRNGRGARSCNAYVVAWVSFGNWLMATDRTGINPVSG"
BASE COUNT         6116 a         6920 c         6346 g         5488 t
ORIGIN      
        1 taatttcagc cctttcatca atacccggcc cggtgccccc gcctctgagc ggtatcttgc
       61 ggtcgccggt acggaagaaa gcggcttgag cagtttctct tcagaagatg ggatccactg
      121 gactttaaaa gatgaaaaaa ttatcaccgg cggtgccttc gactcacaga acctggcttt
      181 ctggtctgaa gccgaacaat gctacctctg ctatttccgg acctggacaa aaggcaacta
      241 tgcaggatat cgctggatca gccgtgcctc ctcacccgac tttgtccact ggtcgccccc
      301 ggaagaaatg gatgcgggca atgcgccgcc ggagcacatt tataccaacc agacgctccc
      361 ctactttcgg gcacctcatc tgtacattgc gctggcagcg cgctttatgc ccggcaggca
      421 catcatgtcc gagcaggagg cggcggtatt gggcgtcgaa aaagggtact acaaggactg
      481 ctccgacagt gtgctcgtga ccagccgcgg aggcacccgc tatgaccgct gttttctgga
      541 aggctatgtg cggccgggta tcgggctgga aaactggact tccagaacca attatcctgc
      601 ctggggaatt ctcccgaccg gcgaaacaga actctcgctc tatatccagc ataactacgg
      661 gcagccgaca gcacggattg accggtatac ccttcgtacc gacggatttg ccagccttcg
      721 ggcaggatgc acccgtggtc ttttgacaac gatacctttt acatttaccg gcaaagacct
      781 ccatattaac ttcagcactt ccgccgcagg ctccgtacgg gcggctttga ccgatgaagc
      841 aggcacagct tatcccggat tttcattgga agagagcaac gaaattttcg gcaacagcat
      901 tgaccgcatc gtccgctggc aggacgaaaa cagcgcggga attctggcgg gcaagaccgt
      961 tcggcttgtt atccagctgg ctgatgcgga tttgtatgca tttcagttca aataaaatag
     1021 gcgacacaat tttgtaattc agcggtataa tatatacagg gtacgttaac tgtgcccgaa
     1081 accgtgtgtg cctacctctt tagaggagtt cctctcatga aacgcatgct ttcgttctca
     1141 ctcaccacca tcgctctcct cgtgcttctt tccggatgcc cctggttcaa gccccccgtt
     1201 ccgcctgtcg atcctgtaga aggagaagaa gtaattgaag gtgaacttcc cgctgagggc
     1261 gagagcccgg aggaaggaga ggcgcctctc tttaccagtg cggacccggc acaacgcggg
     1321 gtttacgatg agactctgga tggtgtgccc acaccggacg ccggagaaga aaacgaaggc
     1381 ggcaaagaat cccgggaact cgttgagccc gacgtgatca gacgggcagg caacctgctt
     1441 tacattctca accagcaccg cgggctcatc attgccgatc tcgacgggca gcgcacgctg
     1501 tcgcaaacac ccacgttcgg atatccccgg gatctgtatc tggtcggcaa caaagcctat
     1561 gtgctggtca gttatgcgca agatgtccgg ctggatgaga aaggacgcta ccaggtctcc
     1621 tacggttccc gcctttacgt ttttgatgtc accgatcctg aagcagttgt ttcactggga
     1681 tccagccgct ttgagggtga tctcatcgac agccgcctcg ttggctctat cctgtacgcc
     1741 gtgtgcagca actatacctg gtattaccct gaggaaacag acgaacccat cgaaacagcg
     1801 tccgtagaaa agagctacgg cgacacctgg gcaatcagtc tggatactgc ccgaccgggt
     1861 gccatgagcc aggtggctaa agttaaattt cagggatacg gaaatctgat tcaggcgacc
     1921 agcgaggcaa ttttctgtgc cacttccgac tggtacagcg acagcaccgc aatcacttat
     1981 gtcgacatca gcgatccccg cggtgctatt gccgtacggg gcacagctgt tgttcctgga
     2041 catctggcag acagatttaa aatggacgcg tgggaagatg tgctgcgtgt agttagcaat
     2101 accggatgga acgaccggaa aaccctgatt accacctggg atctgaaaga ccccgatacc
     2161 tttgcacagc tgggatcgac cgaactggaa agtgccgccg gtgatactac gtatgcgaca
     2221 cgctttgacg gtgcccgcgc ttatattgtc acctacctca cggtagaccc cctgtatgtc
     2281 gtggatcttt cagatcccgc aaatccgcag gtgctgggcg agctgaaaat tccgggctgg
     2341 tcaacgcata ttgaaccccg gggcgaccgg ctcatcgctc tgggcgttga tgatgaaggc
     2401 ggcggcagac gggtcatggt cagtcttttc gatgtgagcg atcccggggc tccgcagcgc
     2461 ctcgattaca ccagcttcgg cgacaactgg agctggtcaa cggcctatga agatgtcaaa
     2521 gccttcaccg ttctggacaa tgccattctg gttccattca gcggctggaa ctacggcagc
     2581 ggcggatatg accggctgca gttcatctcc tacaaccgcg atcgcctgca ggcgcagggc
     2641 tatgtggatc tgcggggaag cgcactccgc gcactgcagt acggcggtta ccacttcgcc
     2701 gttacacagg aacagttggc tgtgattgat gcctccgacc cgaacacgcc ccgtatcaca
     2761 aacagcatca cccttgccga aaacattgcg gatgtcgtcc cgctgaaaga cggatgggca
     2821 ctggaaatta tctgccggta tgactccgcc gacagcagcg tccgcgctgt gaatgctgcc
     2881 ggtgagacag ggcaggaagt gcttcttaaa acgtcctcca tcacggagag ttttctttgg
     2941 aatgacactg cggtgctggt ggctgccggc tatgattact atccggaata caacagtttt
     3001 taccgggtct atctggtgaa tgtttctgat ccgctctccc cggttcttga aaaagaaatc
     3061 ttggtgaaac tgcagccctg gtactacggc tggtggatgg gctacgacat ggttgcttcc
     3121 ggaaaacagg cgcgaccggg atactacccc tactactact attatgatcc ttcgcctact
     3181 gcatttctgg ctggagacta tcttgtgatg cgcggatcag gcgaccgctt cgaccggtac
     3241 ctcggcgcat cctccgctta ccagggtctc gccgtgctga atcttaatga cccggacaac
     3301 atgacctacc tcggtctggg cgaccctgcc ccggaaagag tgagcggcga cggaaagcag
     3361 atatatctga cctatcgcaa gacgctggcg acttcctccg gcaacgccta ccgctgcgct
     3421 tattacctga gcactttaga tcccgccacg ctggatgaaa gcgccagagt caatgtgccc
     3481 ggcatcttcc agcaccgtgt tcccggcact gatattctgc tgcttaaaga tacccagtac
     3541 ctgcgggacg gcaacttgaa ggaactgctt cgaagcggca ttctctcgga aaacaaattc
     3601 tcgctgcagg attccgtcaa cctgccgtca tacggcacgc agcttgccct ctccgataat
     3661 tttgctggct acgtggggta tatttactcc gaaaacagcg atgtcccgcg tgatactctt
     3721 ttgtcttcgg gcaacgacaa agcagcagaa gaagcccggg gctacatgct tggcggcatt
     3781 tttgtcaacg ccaaaggcgg cttcaaaagc catgaaagaa agaatgtggg agacgtatgg
     3841 tgcaaccttc tcggcgtgag aaaaggctcc gtatttcttt ccgtctcagg tgccgctgct
     3901 tcccagtgga acttcaacga acatccgccg gaacacgtaa ccctcttccc tgtcatgggg
     3961 tatccgagta agctgcgtgt gggcaaagat acagcatacc tgccgttggg gtatggcggc
     4021 ctggcatcct tcccgctcta gtcctctccg aagccacaga cagataccat cagtcccgga
     4081 actcaccagg gttccgggct gtgttttttc tccatgacat cagcacggcg ggcgcgcact
     4141 tcttcataca aatgttcgta ttgatccaca atcagatcaa ccgaaaattt ttccagcgcc
     4201 cgttttctgc ctgcctgtcc cagcgccgtc ctcagttcgg gttgtgtaag gatggcacga
     4261 gcgtactcag ccatactttc aatatcccca acttcgcaca aataccccgt tttaccatga
     4321 actaccactt ccgtcacacc gccgctgttg gtcgcaatta ccggcacttc gctcgccatc
     4381 gcctccaaag gaaccagccc gaaactttcg tgttcgctcg gctgaataat caggtctgcg
     4441 cagggaagca aatcttccac gttggcgcaa ttacccagat aactgatgcg gtcttccaca
     4501 cccagctgcc gggcaactcc tgccgccgag agacgttccg gcccttcacc caccatgatg
     4561 aggcgggcgg aaacatcatc cagaatccgc ttgaacacgc gtatcacatc agtgacacgc
     4621 ttcaccggtc tgaaattcga caaatgcatg attatttttt cgtccccccg cgcaagagtg
     4681 ggacgattga tcttccgtgt atgaaaccgg gtgggatcca gaaaattata aatcgtgcgc
     4741 accggctctt tcaggtcaaa ctcacgctcg gtttcgcggc ttaaccagtc cgacacggct
     4801 gtcaccgcac aactgctctc catggcatac tgcgtcgcac gataaaaaga cggatggctg
     4861 ccgaccagcg taatgtcggt gccgtgaagc gtcgtaatca ggcagaagcg tttttctgcc
     4921 ggaagcatgc tgcgcccgag cagtgccgcc accgcattgg gaatcgcata atgggcgtgc
     4981 cagagctgaa tatcatattc ctgcgcaacc tcggcaattt tactggacag cgcaatagaa
     5041 taaggaggca gcttgaacaa cggatagttg atggtatcca cagaatgcgc aaaaatattg
     5101 ggctcataat gaggcagccg aaaagggggc tccatggtga caaaatgaat ctcatgaccc
     5161 cggcgtgcca atgccagccc gagctctgtc gctactatcc cgctgcctcc cgttgtggca
     5221 tggcacgaaa tgcctatttt cataatcaga ttttcctttg ccagcccaca aagagccggt
     5281 cggcactatt ctacagcagg atagggagca cgcgcctttt ctatcaggcg gatttcgaag
     5341 agctccggcc agtatttacc cgtgacaaaa agacgctcgc catctctgtc ccaggcaatc
     5401 ccgttcagca catccgcccg ggcaatttcc ggtcgtttca ctaaagaact gcagtcaatc
     5461 cagcccgtca cgtaaccgct ttccgggcag ataattgcta tccggtgatc ataccagatg
     5521 ttggcgtaca ccaagccgtt tatgtattca agttcattca gtttctgtat gggactgctg
     5581 ccgtaacgga catgaacact ccccttctct tcaaagctgt tcatatccag aaaacgcagc
     5641 cggctggtac cgtcgctcat aataaggcgc ttgccgtcat gggtcagccc ccagccttca
     5701 cccagatagc cgaaatcacc cgtctgctcg aaactgtcca gatcgtagac aaatcccatg
     5761 tttgagcgcc aggtcaactg aaaaaggcgt ttcccctgaa tagcaattcc ttccccgaaa
     5821 taggaggagg acagaagatg ctgacgaatc agctccccgc tttccagggc aacctgacgg
     5881 atggaggact tgccgtgcat gcccgtacct tcataaaggc agccattttc aaaaacgagc
     5941 ccctgcgtaa atgcacctga atcatgggga taaacggtga caatttcaca ttcgtaatag
     6001 atgatttcct ccggaggcgc ataatccgac gggggcagcg gggcgcagca tccgccgaaa
     6061 aaactccagg ttataagaga cgccgctaaa cggaacaact tgcggcgtgc ggaataaaaa
     6121 aacataaaca aaacgacagc ctctcactcc ggtgatgggg cgcccatgaa acccggcgtc
     6181 ttcaaacccg ccgggcacat cgcgtccggg caactgcggt cagcacagcg atatacggga
     6241 caaccagtaa aagtacatgt tttgatcccg gcaacgctct tttttttact tggttgcttc
     6301 tttattcgta accgcaaggt tgcggtccag ctgcaaaaag atttgtgttt tgctggtgtc
     6361 gttgatgaca ggctcacaaa attccagcac gatctcttcc attttggatg ctttcaactg
     6421 cgcgcgaata ttttcccgag cctcttcaaa agaaatggcg tgctgcgact ctgtatccac
     6481 cagctgaatc agatgcatac cgtgctggct tcggatcggc tcgcttaatt ctttgggttt
     6541 caacgcctgc ccggcttcca cataaaaggg cggaagcaga ttgaccggca gcatgcccat
     6601 gtctccgcct ttctccgcat caggtgcctc cgagacgctg cgcgccactg ccgcaaactg
     6661 ctctcctgcc tcgatgcggg acttcgcatg cctgatctgt tctgtcgctt ttgtccacgc
     6721 tttgtcatcc gcctttgccg cggagggttt gggcacaacc agaatatgat tcaaatgcat
     6781 gcttccgggc atctgaaaga aattgggatt ctcatcgtaa aatttcttta cttccccatc
     6841 ggtaacagac agttttttct ctttagccag ttcttccgcc atcttctctg caagaagctg
     6901 ccggcggatg ctgtcaagcg cttcttcccg gctttgacgt gcccgttcaa gaacctgggc
     6961 ttcccctact ttttccccgc tggtttcctc aatatactgg gaaaaagcac gaagtttttc
     7021 ctgatattcc gtctcgacgt ccttgctggt cacctgaatt ttgcggcgcg acgcttcctg
     7081 tacaagaatc tcttcgcgca gcagttctcc aagcgtagtc aaacccgcat gaacccggaa
     7141 ttcatctgtc agattctgat ctgccacgag ggacatcaca ttgcgcaaat gcctccggta
     7201 ttcttcaaga aaataccgcc cctccacggc aaccccgtta accagggcaa caggtccggc
     7261 agggacagaa cgctcgacta tatccaattt tgcaagatcc ggcacctgag cgcctgcaga
     7321 aaagacaacg agacagaaga gcacggaaag cgcaactatt tttttcatga atcaatctcc
     7381 ttcatgtcat tgtagacaaa gaggcgcaag gacacaaaat tggatgagcc cgttttcagc
     7441 cgtcaacctc atacccgcgg gctgtttcca atatgtgcac aagatttttc agtggagtac
     7501 ggggcaaaac accgtgcccc agatttaaaa tatgaccgcg gctttgccct tcagcaaggc
     7561 attcttccgt cgcccgggca atcgcgtctt tgcttccgtc tgccaccagg acgttgtcca
     7621 cattgccctg caccaccagg gaatccccca actgtgcccg ggcggccgtg atggagaaac
     7681 aggagggaag cgaaaggaca tcggcacctg ctgcgccaag gtcttccagc tgcacaccgg
     7741 cttcatccag tctggaaaaa agaatcgtcg gaccgaagcc cttcaaggca gcaaaaatac
     7801 gctgctggga cgggagcgca aagcgcagat actcgtcgcg gctgaacagg tgggcacacg
     7861 attcgaaaag ctgtaccgca tcagcaccgc cggaaaactg atacttgaga taatcaacgg
     7921 tcatgactgt aagaagatcc agcagcgcat ccacccgctc cggacagttg cgcatcatgc
     7981 ttcgaagtac agacagttct gaggacgggc tcttgccttc tgaaagaaaa gcaagcagcg
     8041 taagaggcgc acctgcgaaa ccaagcaaag ggtattcctg cccggcgctt tggcggatca
     8101 gcgtcaggct ctctccaaca aagggcatcg cttctctcac gtcgcagcga tggagcgctc
     8161 ccggctcagt caggaaccgc tccggattcg ccagaacagg cccgggagca aagcggaaaa
     8221 aggctcccat tccttccaga atagtcagga tgtcctgaaa aaagatgatg gcatcgacgc
     8281 cccaggcaag aggcagaagc gaaatccgcg ccgccagatc aggcgatcgg aacaggtgct
     8341 ccagaggaag gccgcttttc tcgcgtaatg ccagataggc cggatcacag cgccccgcct
     8401 gccgcataaa ccatacaggc acacgcggtg ttttttcacc acggagcgcc tgcataaaaa
     8461 gacttcgctt attttctgac atatctcctc atcagagcag aaaagaataa catccggcct
     8521 gagatgacgg aatccgcctc aaaagcgcct cattcgtcct ggaaatcaag atctacctct
     8581 tcttcgtcca gatcagggga aagatcttcc tcttcctcgt cttcctcttc ttcatcctcg
     8641 tcagcgataa cggtgaggcc gtcatcggtg tcaccatctt tatcatcgcc gccgtcactc
     8701 aagacaacag gcttccgatc ttccgcctcc tcgtcatcta tttccaactc ttcttcgtct
     8761 tcttcgtact cttcacccag gtcttcctct tcataatcga catcggcgcg gggaacaaga
     8821 gtctggcgtc tttttgtcgc ttttgatttt gcactaatct ccagcagact tgcctccggt
     8881 gagccaagca gaaccagcag attttctgca cagccctctg tcggacattt gaaatcaagc
     8941 ttttttgcgc cccagtctac atacttacgt ccgcattttg tgcaaatgta tttatcagcc
     9001 acggtcaatc ctttcgcaat aaatactcag ttaaagtctg ctaaaaactt acatttctga
     9061 tgaggaaccg cgaaattata gcaggaatac gaagtcctgt acatcttttt attgaaatcg
     9121 ccacgtcttt tttttcagaa ggaaaacgca cgaaaaaaca gagccacttt gaaagaaaaa
     9181 ccgggtgcac cagaaaaagc gtacacccgg aaatttcagc aaaaacaaac aggcaatcag
     9241 gctttggcgc tgctcctgcg cggggcgcgc tcctctgctt tacccgactt gcgactttgc
     9301 gtactgccac cttcagccgg acgcttctcc atcagataga ccagtgcgct ggcaacgaaa
     9361 attgaagaat aggtaccgaa aacaataccc agcagaagga ctatggcaaa gtcgcgaatg
     9421 gcttcaccac cgaacacagc cagcacgacc accacaaaaa gagttgtgat ggaggtcagc
     9481 agcgtacggc tcaacgtgga gttaattgca ccattaagga tatctataaa acgatagcct
     9541 ttgccgcgct gtgcctgaat atcttcacgc acacggtcaa ataccacgat agtatcgttg
     9601 atggaataac ccagtattgt gagcagcgcc gcaatgacat tcatgctgat ttcgccgccc
     9661 agaagggaga aaacgccaac cgccacaata aggtcatgcg ccagtgctgc cacagcgccc
     9721 agcgcaaagc ggaattcgaa acgaacccac aggtaaatca cgatgaaaag caatgcccag
     9781 aaaatggcgt tcagcgcatc ccaccgcagc tgtgcaccca cggaggggcc tacagtctgc
     9841 tcgtcttcaa caaccacacc ctcggctgta ccggcagcac tcaagggtgc cagtgcctgc
     9901 tgaatgcgct ccgctaccgt ctgaagctgc tcgctccccg cttcggcacc ctctgccgtg
     9961 cgggtaatat cacccacgcg tacaatgaaa acattggcat tgccttcgcc gctatcgccg
    10021 gagctttcct gcacgaccgg gttggtgaag ccgctggcgg acagagccgc acgcacatca
    10081 cctaccggca cagaatccgt attttccaca tgcagcatca ggttggtgcc cggctggaag
    10141 tccacgccga aattgtccat accacggatg gcaaagcacg tcatgccgac aacaatgacg
    10201 gcaccgctga aaaggaaggt aaagttgcgc agttgcagga agggcacccg cggattttca
    10261 ggcacaatgc tgagcatcga tacttttttc atgacgcctt ttgccaggaa aaagtcaaaa
    10321 agcgcacggc cgataaccag cacagtaaac acgctggtca caataccgat actcaaggtc
    10381 accgcaaagc cctcaatcgg ccctgtaccg aattgcatca gtacggcagc agctatcagc
    10441 gtggtcaggt tggcgtccac aatcgtgacc gtggcgcggc ggaaaccgct ttccactgaa
    10501 gacaacaccg tgtgccccag tcgaagctcc tcccggatac gttcgaaaat cagcacatta
    10561 ccgtccaccg ccatacccat ggtcaggatc agaccggcaa taccgggaag ggtcagggtc
    10621 ataccgaaat acgccatggc ggcaataatc atgagggcat tcagggcaag gcagatacag
    10681 gagataaggc cggcgccttt atagtaggcg atcataaaaa gtgccaccag cgtcacgccg
    10741 gcaagggagg cataaacacc actgcgcact gtgtctttac caagactggc acttaccgta
    10801 cgggtgaact cttcacgaac gggcacaacc atggaacccg agttcagggc aatggcaaga
    10861 tccaccgctt cctgtgcctc aaagttgcct gagatctgtc cccggccacc cgcaatgcgg
    10921 tctcgaattg tcggcgccga cacaacaaca ccgtccagca caatcgccat gggcttgcct
    10981 atattggcac ctgttgcatc agcgaagttg acggctgcgg aagcgccgaa agaaaaaagg
    11041 atctgccagt agggcatatt gctctgatcc tgaatggctg ctgccgaggt cagcccctca
    11101 ccactggcaa tcggcttgcg cgcaagaaca tacaggtgat attcctgatc ccgcgcgtag
    11161 ggcttgggct gctggctgaa ggcaatgatt ttgtcttcag gaatgacgcc gctctcttcg
    11221 gcacggacaa gaaccctgcg cacccgctcg tagttttcca cactcacagt cagcacatca
    11281 gggcgcatac tcgtcgtttt tacaaacgga ataaactcgt tggggaaggt gtctctgatt
    11341 ttttcgaaaa cagggcgcga ctcatcgggc cccgcaacaa tatgaaaatt catctgagcc
    11401 gttttcgtaa tcaggttctt ggcgcgctgc acatcttttt cgccgggcag ctgcacctgg
    11461 atctggttgg tgccaagcgt ctggataatc ggctctttcg cttcaaaatc attgatgcgg
    11521 cgcgtgatct gctgcagcac aatctgctgc acttcttttt caatattctc atcagaatac
    11581 cgccgctcgt tgcggtattc gtccagcatt tcctgaggca gatctttaat gtcgaagccg
    11641 atcaccatat ggataccgcc ctgcagatcc agcccgagat taatgacctt gttccggtca
    11701 cactcaaacc aacggcgggc acctacccat aattcctcaa aatagcctgt ctggcgacgg
    11761 gcaagatcat cgtcctcttt ctgccatttc tcaagacgtg cggcacggct ttcatccgac
    11821 aacagcatcc atcccaccgt cggataaaca tacacaaagg ccagcaccag agaagccccg
    11881 atcagcaggc tgcgatatat gctcttggac atgcgttact ccaactgatc cctgtctgca
    11941 gggatacaca aggccgcatc gacggcggcg gttatacgat taaacgggac aggtcccgct
    12001 gttccggcgc caaactgccg aaacacactg ttatatactt cgcctctttg tcggaaaaga
    12061 caaagaaaca caaaaacact tcgttactat ttcgtttctt tcggaagcac ctgcgctatg
    12121 gcgccgcgga caaactccat ggttacattg tcatccaccc gcagcacaac acgatcatcc
    12181 gacaacccca caacgctgcc acagactcca cctgtcgtga ttacattgtc gcccttggca
    12241 aggctgccaa gcatttcacg gcgttcacga tcccgtttct gctggggacg aatcataaga
    12301 aaatacatga tccctatgaa gatgacgatc atgggaagca tgctgcccaa aggattgacg
    12361 ggtgccccgc cagcgccctg cgccgcacct tccgctccgg caacgattaa atgccacacg
    12421 tatgaatctc ctttaatcca ataaacatac ccggactgag cagtcgggcc tcctgattcc
    12481 gggcacagca caccgcctcc cgctatggat tttggcggtt ttacttctgg tggtagtcct
    12541 gcaaaaaagc cgttttgaag ccggtatagg taccgctgca tagtgcacgg cgcgcccgga
    12601 ttgccaattg tatcataaaa taaaggttat gtaaagtatt cagcctttga gacagcagct
    12661 cacccacccg aaagaggtga tgcagatagg cgcgggagtg attgcggcaa gcggggcatc
    12721 cgcagtcagg atccacgggc agtccgtctt tggcaaaacg cgcattcttg atgttgatcc
    12781 tgccgtaccg ggtgaaaaga ttgccgtgcc gtgcattgcg cgtcggcatc acgcaatcaa
    12841 acatatccac ccctttttca atcgccgcca gaagatcctc cggtgcgcct acccccatca
    12901 gataacgcgg cttgtccgcc ggcagaaatg ccgtcgccca gcctgtggcg gcctgcatct
    12961 cctctttgct ttcaccgacg ctgacaccgc cgatggcata gcccggaaag tccagctcct
    13021 gcagggcttc ggcactttcc cggcgcagcg attcataaac gctcccctga acaatcccga
    13081 acagtgcctg tttctctgta tcgccttcaa gccagcgatc ccggcagcgc cgcgcccaac
    13141 ggcttgtcag acctactgaa aaggcggcac ggtcataggc cgccggatac tccgtgcatt
    13201 cgtcaaaaca catcatgata tcagcaccga tgctgcgctg aatatcaatg gcactttcag
    13261 gcgaaaaaaa atgggcggat ccgtcaacag ggcttttaaa agcgacccct tcctccgtca
    13321 ctttgttcag tttggacagg ctgaacacct gaaacccgcc tgaatcggtc agtactgggc
    13381 ggttccatcc cataaaacca tgaattccgc gaaaataatc cagcacgtca acgccgggac
    13441 gtaaatataa atgaaaggtg ttcccgagaa taatctgtgc gcccagcgct tccaattcgt
    13501 tgttactgag ggatttcacc gatgcctggg taccgacagg cataaaaacc ggcgtttcca
    13561 ccgtaccatg aggcagttca agcactccgg cgcgggcact gctgtgggga tcctgatggg
    13621 ttactctaaa tttcatattg aaaaaaatgc tccgttgcct cgcctgcgac ttaatcgcgc
    13681 cgcccgggga gaagcgggag cggatcgacg ggttggccgt tttttcgtac ttcaaaatga
    13741 agatgcggtc ccgtggagtt tcctgtattg cccactgtac cgatctgctg cccctgccgg
    13801 acaggctcat tacactctac ataccggctc tgcaagtggg cataagccgt ttccaggtga
    13861 ctgttgtgcc gaagaatgat caggtttccg taggcaccca tcaccccgga atacaccgct
    13921 atacccgacg agacggcgaa cactccttca ccgggcgatg ctgcgagatc gaccccctta
    13981 tgccacctgc cctggctgcg cgcttccccg aaaggcgacg tgatccgggg ctggtggtgc
    14041 cgcaaaggcc agatcaggtg tgcttttctg taatcttgcg ccatatatcc ggaatcgccg
    14101 gatgccggag gcggctggag cacctgtatg cgccgggcgg aacgggcgca cccggaagcg
    14161 gagagcagca gcaccagcac aaaagccggc aggagccggt tcaggcacag aaaaggagtc
    14221 caaaaacggt cgaaaccctt ggttttatgg cataacggca tcggattcat catcgcgcac
    14281 actataccac agatgaattg aaggggacaa aaggcgtttt aagacggagc gcttcgcttt
    14341 tttacgataa aatcagatat aatatccctc acttgggcag aagctgagga agactcggag
    14401 caaatcatgt atcgttcaaa aataaacagc gctcttattg tgcctgttct attatgtttt
    14461 gctctgcttt tgaccgcctg ctcgagccag acgggcgagt ctgtccatat cgtgggctca
    14521 tcctcggtac agcccttcgt tgaattgctg gcggaaagtt acttgaaaaa caatcccgaa
    14581 gaaaatgttg atgtacaggg cggcggctcc accgccggac tgcaggccgt gcgcaccgga
    14641 cttgcaaata tcggcacaag ctcgcgcatg cttaccgccg cggaaagtga aacctttgac
    14701 agtgccgttg tcgctcttga cgggctcgcc gtagtagtta atcctgccaa ccccctctcc
    14761 aaaatcacct cagccgagct cagagatgtg ttttccggca aaatcgccaa ctgggaagtg
    14821 ctgggcggag acgacagacc gatcaggcct atcaccagag aggccggctc cggcagccgg
    14881 gacaatttcg cctcgctcgt catggaaggg aaccccattt cacggcgcgc tattacccag
    14941 gaatccaacg gcgcagtcaa agaactcgtc aaaggcgacc ccgccgccat cggctatatt
    15001 tcactcagta tgctgtctca tgaagttaag gcactgcttg tcgatgaaga ggcccctacc
    15061 attgaaaata ttcagaaggg gcactacgcc ctgacccggc ccttcctctt tgtcacaaaa
    15121 ggtgacatgt ctcccgaagc ccgacgcttt tttgagtatg tactctccga tgaaggacag
    15181 gcaatacttg aagaagaagg actggtacgc aaaccatgag acaggaaaaa ctgattgccc
    15241 tgatccttca ggctgttgcg ctctccgctc tggcagggct gctgctgatc acagtcttta
    15301 tttttaagga agggctgcct ctgattctcc gaacggggct gaaagatttc ttcttctcct
    15361 ccgattggga acccgatgcc ggccacttcg gcatcatgtc catgattatc ggctctcttg
    15421 ccgttacact gggcgccatg cttatcggag gcaccctcgg gcttggcatg gcaattgtgc
    15481 tcacccagtt ctgcccgccc aaacttgcct cctttttaaa accgaccatc gaattgctgg
    15541 caggaatccc ttccgtcgtt tacggattca tgggggttgt gacccttgtt ccgctgatcc
    15601 gcagttatct gggcggcccc ggtctctccg tgctggcggc atccatcgtg ctgggcatca
    15661 tggtactgcc cacagtgacc agcatctcca ttgatgcact gaaagcggtg cccagaactt
    15721 attgggaagg ctcggtggct ttgggcgcaa cacgctggca gacaacccat atgctgatgc
    15781 tgaaagcggc gcgctccggc attgtcgccg ccattattct gggaatgggc agagcgctgg
    15841 gcgaaaccat ggcggtcatc atggtcgccg gcaatgccct cgaaataccg ggatccgttc
    15901 tggatcctgt gcggactctt accagcaata tagccctgga aatgggctac gcttcaggcg
    15961 agcacagaga ggcgcttttt gccgcaggcg taaccctttt cgctgtcatc atgatcttca
    16021 acaccattgc gctgaccttt gcgcgccgaa ccataagaca ggggagaggc caatgaaacg
    16081 catctctccc gccatcaccc agctgatcgc tgtcggctgt ctctggacat tcaccctcct
    16141 gacactttcc atactgctct ttatcattgc ctatatcctg attcagggac tgccctggct
    16201 cagctgggaa ttcctctccc aggcacccga aagcatggga cgccggggcg gcattttccc
    16261 catgattgta ggaaccttca tggtcgcggg actggcagta ctcatcgctg cacccatcgg
    16321 catcgccaca gctgtctatc tgaccgaata tacccgggaa ggaaaattca cttttatcgt
    16381 ccgcttcggt gccgactgtc tggcaggaat cccttccatc atttacggac tgttcggttt
    16441 cgtctttttt gccattgctc tgggcatggg gctcagtgtc atttccggtg ccctcaccct
    16501 gacgctcatg attctgccca ccatcgtgcg caccgctgaa gaagcgatcc gtgccgtacc
    16561 gcccgcctac cgtgaagcga gttacggcct gggcagtacc cgctggcaga tggtcacccg
    16621 ggtggtgctt cctctggcgc tgccgggaat ctgcaccggt attgtgctct ctctgggacg
    16681 cagcatcagc gaaaccgctg cggtcatgct cacggcagga tccgcgctgc gcatgcccca
    16741 ttccctctcc gattcaacaa gaacgctggc actccatttt tatatccttt cacgggaggg
    16801 gctctccatg gacaaagcct atgccaccgc ttccgtgctg atcctgctga ttctggcgct
    16861 caacctcttc gcttactggc tcatgcgcca ggtaacacgg aggaacggat gatgcctccg
    16921 gaacagaaca gccctgttat tgaagtgcag aacctcacag tcacctctta tgacagaacc
    16981 attctggacg gactcagtct gcctgtttat aaaaatgaac gtcttgccat tatcgggccg
    17041 tccaacagcg ggaaaaccac ctttctccga tgtctgaacc ggcttaatga tctgcagcac
    17101 agctttaaag tgagcggaac cattctcctg aacggcagca atattttcga tcccggcgcc
    17161 gataccgctc agctgcggcg aaaagtcggc atggtctatg ccgtaccggt gccgctgccc
    17221 tggtcaattt atgaaaatct ggtttacggc cccagactgg cgggcgtgcg ggataagaac
    17281 aggctggacg aactcgtcga aacatcactc cagtccgctt tcctttgggg cgaagtcaaa
    17341 gacaggctcc atatgcctgc caaaaatctc tccggcggac agcagcagcg cctttgcctc
    17401 gccagagtgc ttgctcttga gccggaagtc ctgatcctcg acgagccctg ctccgccctg
    17461 gatcccatct ccacagcaaa aattgaagat gcgctcttcg aactcaagtc aaggctcgcc
    17521 attgtgctcg tcacaaacaa tacaaaacag gcggcacgcg cctccgaccg taccgctttc
    17581 ttcctcatgg gaaaactcat cgaaatgaag gaaaccggac ggctcttcac aaacccctcc
    17641 gataagcgca ccaacgatta tctggtcgga caatttggct aggagtccca tggacgcaaa
    17701 gattgaaaca aaaaatctga acctctatta cggagactac cactccctca agggagtgaa
    17761 tatcgccatt ccgcccagaa gcatcaccgc cattatcggt ccctccggat gcggaaaatc
    17821 aacgcttctc cgctgtttta accggatgaa cgagctgatt cccggcgtca tcattgaggg
    17881 tgaaatcctc ctggatggag aaaatatcca ccagcagggc acagatctca tccggctgcg
    17941 caggcgtgtg ggcatggtat ttcaacggcc gaaccccttc cccctttcca tcgccgacaa
    18001 catcacctac gggctgcgcg tgcacgggac accgcccgga cagaaacaca gcgatattgt
    18061 ggaaaacagc ctccgcgcca cgttactctg ggaagaagta aaagacaaac tgcacagccc
    18121 cgcactggcg ctctcgcccg aacagcagca gcgcctctgc atttcgcgcg tcctcgccat
    18181 tgaaccggaa atccttctca tggatgaacc ctgttccgcc ctcgacccca ttgccacaga
    18241 ccatatcgaa gatcttatgc gcgatctgtc agaaagctac accattatta tcgttaccca
    18301 taatatgcag caggcatcac gtgtctcctc ctggtccgca ttcatgctca tgggcgaact
    18361 catcgagttc ggacccaccg tgcgcatttt caacaacccc agagataaga gaacggaagc
    18421 ctatatctca ggccgttacg gatgatccca acccaactga agcaggagta ccgcccatga
    18481 ccaggcactt ccaacacgaa atcgaaaaac tgaaagtacg catccttgcc ctggctgctc
    18541 tggtcgaaaa atcggtgcga aacgccgtca ccgccctggc cgaacgcgac accgaaatgg
    18601 cacaggttgt aatcggcgaa gacagagaga ttgacctggt cgaagtggat atagaagagg
    18661 aatgccagaa aattctcgct ctccatcagc ccgtggcgca cgacctgcgg ttcattatcg
    18721 ccgtcctcaa aatcaatgcg gaactcgaac gcatcggtga tgccgctgtc agcatcgccg
    18781 agcagatctg ctattgggtg aacgaagggc acacggacgt atcctatgac ttcgagtcca
    18841 tggcgaaaaa ggcgcaggct atgcttcata acagcctcga cgcactcgta aacttcaatg
    18901 cgcagactgc ctttgaagtc attgccgaag acgataaagt ggacgaaatc tacggggacg
    18961 tacaccgctt tgtccggcgt ggcgtacaga gtgcgcccca tcagtcggga ggattcattc
    19021 atctcctcgc catctcccgc catattgagc gcatcgccga ccacgcatcc aacattgccg
    19081 aagtggttat ctatcttgtc gagggacgca tcatccgcca ccgcgccaag gacgccatga
    19141 aacaggacac cttcggcaac ggctcacaaa gctgagtttc tgctctctgc tgaaaataag
    19201 gatggctcac cctgccggga aaagaggaaa cgggcagaag aagctgtaaa agaggtggga
    19261 aatggcgcgc ccagcaggac tcgaacctgc gaccttctgc tccggaggca gacggcaaga
    19321 ggttataaca tgttgaatta taaagagtta gacgggcttc ctgtgaacac ttgtactcct
    19381 acttgtcaaa atttcaaaaa agtgttcacg ggaagacgtt tttcagcatc aaaaaacatc
    19441 cggagggaac cctaggttct tcccggagga gcggatagca ctggctacgc agcccacccc
    19501 ctaattttta tccctacagg agtttaattt tgcaaaaatg caaaaactca agtgcaagaa
    19561 ctgtaagtga aagtcccccc aaaagcagga gcactagact atgacagaga aacaaaaagc
    19621 agtgcacact atccccttca agcagtggct taaagaacag atcccggcgg gcatgtctga
    19681 tattcagctg ccgtacattg aggaacatct tcaggggaaa acattcactc gatcctattc
    19741 cagaaaagaa ggtgaggaga tacggtacgt ctatgacatg actccagtcg gctatgttcg
    19801 ggcgtgggtt cgcgaagtgc acaaggataa taaaaggctt ctggagtatg ggcttcaggt
    19861 ttgtgatgac tttgaaaaag gactccttca agtttacgat cttcccggga aactgagagg
    19921 ctacattgaa gactacatgg aagaagtgtg catcgaagac tcccctttag ataatcccgt
    19981 ctggtatatg tcttctctgc tctcgtattc cctccgcttc aaagtggctg ccgctgttgc
    20041 tgaagatggg attaaagaag atgtgactcc cacagagttt tgtttatggg caagggcgat
    20101 gaacctttat attaatgcca gtggagcggc tttaatcgaa accagagaag ccattcttca
    20161 ggtgattcat gggcaaaaga agaatgctga gcggcagcaa cccccttctt taaaccttgc
    20221 taaaaaggca ggagctacga ggtgggaaga gcttgctgtt cgactgaagg gcgatgcaat
    20281 cgaatacagg cacatcgacg gggacacctt caagcgcatt aaagccaagg gaattttttc
    20341 aaagctaaac tccacacagt acgaaatgtt aatcaattta gcaagaggag agggggtaca
    20401 cacagaccca cgaggggtgg ggctggatac cacgttgagc aggctaaatg accgacttaa
    20461 agaagccttc gggttatcag agaatccctt cttccgcgcg ggcaatgctt tcttaagcag
    20521 gatagggaaa ctctggcttc atcgcacctg agggggttaa attttcttac acatgtaaga
    20581 gacaaaatgc cagacggtgt aaattttatc ccttttacgc tcaataacat accgctttca
    20641 ttctttcagt ttgcttgcag tttaggggta gtgtagaggc ttgtaagaca gtcttacagg
    20701 caaaaccttt acaaggatct acactatgtt gttaaacgct gtagaagttg gaaagaagtt
    20761 gggcgttgca aagcggacgg tgtggcggct tcgggattcc ggcgctctgc cttcccctgt
    20821 gcaagtaggc ggctctgtga ggtggcgggc tgatgtgatt gaggcgtggg tgaaggcgaa
    20881 ctgcccgcat gtccggaaaa ccgggtggag ggcagagcaa tgaccagacg tacagcaata
    20941 cggaaatatt ataaatggct ccattgtggc agcgcttacg gcgtggagcg gtgcactatg
    21001 accacgcatc ccctgtaccc ctaccgatcc gggaagatgc cggaagggaa gacaggaaaa
    21061 gaccgtgaca aagccatccg tgaaaactgc ctgatttgtg ccggtgatcg agcagaaaga
    21121 aggaattgcc cgttcacgga ttgctccctg tgggaatttc gttcaggaag ccctgcaact
    21181 cgtcctatag aggggtctgg agcgaaaact caggctcacc catacttaac cccgcctgaa
    21241 ggagatgtct ccccaaaacc cccttatagg ctcacttttg gggatgagga ggggcaggca
    21301 tgagtatagc aaaagaaaaa agcccaagtg cggcaacacc cgggcttcac aagaacgtgg
    21361 ctccaccact cacggaacct ccttccagtt taccagaaca caccagctgc ttcaactacg
    21421 gcgtctgtgt aaaagggagg tatgacggat cccgggaact ggtgcaggac tggaaggaca
    21481 cattcacaaa ataccttgca tgtgaagagg aagagacaga agcgtatctc acggcgttct
    21541 catatgggga tgactttctt ccccattaca aacaataccg gagcaacaaa ggcttcacgg
    21601 gttccgcctt cgggcacttc ctgtgttttg atttcgactg cgcagaagat ccatcggatg
    21661 cgattgctca ggcgcagcac ttcctgaacc actgtgcaac aaactacggt gcggatatgg
    21721 atgctgtgct ggcggggttc agtggcggca aaggggcaca tatctatttc ccggtgcctt
    21781 cctgtgctgt tccttcggtg gacttcggaa acatctgtct ggcgattgca aagcggttca
    21841 aggatccata caactgcatt gatctgaaga tctacgacca tcagcggatc ttccggctgc
    21901 cgaatacccg gcaccccaaa agcggcaagc tgaaggtgat gtaccgggct tccgatttcg
    21961 ttgaattgga tcctctttac attctcaaca aagacactgc tcagaatgct gatttccttt
    22021 tttccgatga tgccggtgaa tggttggctc ccgtctggaa ggcggcggtg aatcagatag
    22081 agaaagaagc agctcctctt ccttcccggg cggagggctt ccgggatctc tccggggata
    22141 cggtggactt catcaaacac ggcgcaaaag aaggtgagcg caatactagt ctcttcaagg
    22201 cagcatgcaa cctttatgag atgaatatga cgcaacctgc agttgagaag ctgcttgcac
    22261 cagcagcacg ggacagcgga ttgtctgaag cagagattca gaagaccctg caaagtgctt
    22321 ccaagcatgc ggagtcagca agagggcggt tccgattgca tgaggcttgc aggagctcca
    22381 agaagttcta tgatctggtt tgcgacattc tcgcaggcgc ggacttcccg ccagaaggcg
    22441 actggggaga gctggagcgg cactatggtg agcacgactt cccccctgtg ggcgctctgg
    22501 actcggcgca gttttcagaa gcagaagtac gctaccttgc ggaacaatgg cttgaaacgt
    22561 ggaaggcaca ggcgacgcaa gaggcgaaga aaacacttga tggcgcgggc gcgcttattg
    22621 aacggcttca ggtgattgag caagcaggca cagatcccgg gctttaccag cgggcttcag
    22681 cggcaccacc acaaggaaat ccgattttcc ggaacgggct gcttccggga tctatcggtg
    22741 ccatctttgg tagtgacggc atcggtaaag ggttcatcat ccttgatttg atggtgggat
    22801 acgcaagagg aaacccactt gggataactt ctattggggc gataccgggc aagaggctct
    22861 ccaccactta cgtttcttat gaagagagta tctccgattt aaaaacccgt ctggacaaga
    22921 tatgcgggga ggaggtggat tacggaaagc ttgaagagga agggatgctg cgctttagca
    22981 cgattcccaa ggaaccattg ttccggtacg acatgaaagg gagtctgctt tcgactgctt
    23041 ttatgaagac tctggaaaaa gccattatcc gggatgatgt ggatctgatg atccttgatc
    23101 ctcttgcttc tgccggcggg ttgcgcgatg agaacaataa tgcggaaatg aacctgatcg
    23161 caacacgtct caggacactt gcagacaaga caggctgttc aattcttctg gtgcaccaca
    23221 cgacgaaaga aggacgggct tcactggatc caagtgccgg cagaggcgca agctcgttgc
    23281 aaggggcaat gcggtggatt gtgaacttca ggagaaacgc agatgacggg caaatcattg
    23341 aggcggcggt cacaaagaac acgcacggag gtctctgtgc tttttccttg ggacggatca
    23401 ccagcggcgt gatacgagag ctgagctcca cggaaatcca gaaacggcta caggctattg
    23461 agatcacaaa ccggctgctt cttcaggagc acatcagttt tctggtggaa catatcgcaa
    23521 agcatgacgg cgaaatcaaa aaaagcggcg tcgccaatgg gagagggaga ggtgctccag
    23581 agttgattaa agctcttaac tgcttgggaa aaaaaataag agcacgtgaa gcagtggaga
    23641 ttattgacat ggcaatcgag gctgaaaaac taagagaaga tgaggacggt catttgtgga
    23701 ttcaggcttg atcatcatcg atcccctata tatatagatg atgatgatga tctcacaagg
    23761 tacctagaga gggggtatat ggaagtaaga cttcctatat acctcctccc tctcagagtg
    23821 cctagtgctt tacttgaaat acatcaggag gtatgctaaa cttttaacat ggttacacta
    23881 agcgacacat tgagaaaagc gattcaggag agcgggttaa gcatgcggag gctttccatc
    23941 aataccggta taaaccggct ttgcctaaca cgatttcttg aagggttcca gatgacaagc
    24001 gataacattg ataagcttgc tgatttcttt gggctggggt tggcgcattt cggcaacgag
    24061 caaaaccagt acatcaatat agggcgatat gtcacgctgt tctttcgaaa acatgcgcga
    24121 aaataaaacg cttaaagatc ctttgaacgc gggtgaaact tggtcgcttg cgcgcctgct
    24181 tgcatttttt tttaacacag ccccttttgg tttttttggg ctggggttgg tgaagaaaaa
    24241 aagggattaa cctatggcaa ctgtattcaa aaaaacttac acggctccaa taccggaaaa
    24301 tgcggaaacg ttcacccgca aaggaaccga atatgcccga tacaaagggc gtgacgggaa
    24361 aagcagagta gaacggctca ccagtgacgg tgcccggctt cttctggaaa ccggaacgtg
    24421 gtatgcccgc attagtcttt ctgatggtgg aagccttact gtcaacacca agtgcaaaga
    24481 caaagccacg gcggaacagt ttgccgcaaa ccttcaaacg gaagaggata aaatcagagc
    24541 aggtgtattc acaaagaagg aagtaaaagc ggcaaggcac gggcaagacg cacttcagaa
    24601 aagcattgat gcgtaccttg gcagtatgac ggcacggaat aaagcgacaa aaaccgttaa
    24661 ggaattccgg cgatatctgg aatctgtttg caaggctctt ggatggaaag ctgtgcggga
    24721 tgttgacaaa gtggctcttg atgcttatct ggaagaggaa cggcgaaacg ggagaggcgc
    24781 ccgttcttgt aacgcttatg tggttgcgtg ggtttccttc ggtaattggt tgatggctac
    24841 cgacaggaca ggcattaatc ccgtttccgg
//
LOCUS       DAOPWB010000035        24586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2015_length_24586_cov_4.953854, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000035 DAOPWB010000000
VERSION     DAOPWB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 24586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24586
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2015_length_24586_cov_4.953854"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKN92_04995"
     CDS             <1..374
                     /locus_tag="PKN92_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNZ48180.1"
                     /translation="LDALESLEGDCTEYTVLFVGLAAAAGIPAKICAGLIYVDSAKSG
                     FYFHQWASVWMGEWIDVDPAFNQIPVDVTHIKLAEGDLFRQARILPLIGRLSITVNEE
                     APRQDDLSEPEEHAGEEPPQE"
     gene            431..3406
                     /locus_tag="PKN92_05000"
     CDS             431..3406
                     /locus_tag="PKN92_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48181.1"
                     /translation="MSEQKQISNDGSAESPGAVSPVPTEKAPARRKRKVFLLFVLILL
                     SVVLFLGQTGLVERQWLPALSQYFTGYALRFERIGLYPLELRNLELRRKGDYEKDPPL
                     LSVSFLKIDWSVTSLFSEKRLFREISAHGIRAIIRDSGALALPGDGAAAKEKKAFAAP
                     AFLLPRVIHFEDLSVQVQNKDTRASLEGLRGSAVIRSFSDLTAAAGGTEIRARWLPGL
                     EKEELSFPEGNISVKAHRAGEEIQAEAHLSIPDLLQFRAEASLTAGEAPALRLNIIEC
                     SAQGRLPGEFVSPFLPVPVFFERLDAAPLSVTAGWSGGVPEAQQLSFDLRCTGLTVGR
                     AESRLYDGDLSLTARTAAEGGGETDWTLQLNRKQQISGTLRAESGTVHLKAEGEGWDR
                     EGVTALLPGAYAPFASWIPPLKKADFDLVARMNSAGAEFEGNLSPRLDSGTELSAHFQ
                     GAYQHAGSGDMTIEAVLGDGSLQGSAGLHPGTGISVQAALKHCSLADLLSFVPAQTGA
                     EAWSGTMDGSLAFSKSPGKEATVKTTLTVGNLFHPSLMPETLEDSLSFVLSAVLSEDL
                     ASLQSAQCDVEAGAFFKAAVGKCRGDLNTGGLHFSFQGEADLMEAGTRAGFSDLWGAV
                     QWETTVAVKNWLDIVLNPATITCESLGWGDYSLPYGEVLTVTCPARLSLQNNRAVLGP
                     VLMEIGSGTSMRAEEITLRSDAVKGKGLFSFTTDFAPVKAKGWLEEAEGRLNAELRDF
                     EWIPEGLQGNLSFSLTPSSFVLPRSLARMSGLAVDGQARLGASPTAEADIVLEELLAG
                     GVKISAAPARLTFQEGKAKVDPLTLGVFGGTLNLSLQWEPFASGFPLTVRSNIDRIDL
                     GVFTDEFKPQSLVLTGIVRGEMFCVLTPSQLRDFSLMLESTENFSMNRDMVEQLLLNQ
                     YMNDVTGSRQVSRMLQSVLGKKPQTAFDRAQIKLGLEESRIAGTALLESKSLNLTVDI
                     KADPPALLEALRIRQEHTD"
     gene            3516..4202
                     /locus_tag="PKN92_05005"
     CDS             3516..4202
                     /locus_tag="PKN92_05005"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNZ48182.1"
                     /translation="MRKIVAVLGAVVLSFALGCVIRTEHKINAHITLDIRHIADQATD
                     VLDYVEGKSDVLPGMEPAPAPVSSWYDRLPSFRLFETAWADSLSQTDSPVVREIAGKM
                     RERNATIAALKKEGCLGENNRGYVELRECDAAKNPEKKNEIQKTLAEENKDRKALYAE
                     IARLNRELPGVNVTKVERIYALERMRRAQAGESVQLPPAGDDFTAIQSSELGKALGAA
                     CQPDAWVIIP"
     gene            complement(4371..5366)
                     /locus_tag="PKN92_05010"
     CDS             complement(4371..5366)
                     /locus_tag="PKN92_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ48183.1"
                     /translation="MKKRRGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQN
                     NLKQWGLIYKMYANEDKGQMFPPIAATYFNNPLDLTGGHDAAIAIGPRLSAVYPEYLT
                     DPAILICPSDGNNSVDSLKDSNGDWVFAHYVNYLDEHGWSMDESYAYFGYVMDKAENK
                     PEWRDTLANSAPNITTLLGILPNPAAVQTSAIVPIQVGKAVDEVLMRFLSGSREAMDK
                     DIENVFAPGGTIPMGNGNGSTVYRLREGIERFLITDINNPAASAMAQSTVQVMFDMFG
                     AGAAVQYFNHVPGGCNVLYMDGHVEFIRYIGNDAQGTGFINSNTANVIGLIAAAW"
     gene            5649..6593
                     /locus_tag="PKN92_05015"
     CDS             5649..6593
                     /locus_tag="PKN92_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ48184.1"
                     /translation="MKHGSFFLLFALILSGCATFRPGAPSGMTDVIAHRGASHDAPEN
                     TLPAFELAIEQGADWFELDCRLSQDGAVVVFHDDNLERITGLADRVDALDFASLPTLD
                     AGAWKGPSYAGAQIPTLAESLDLAKGRIGVYVEIKNAANDGALLEQILAMSEGVPVMT
                     DAHARKILAAIEESGTKNLELTRKTLALIRERHMEKEIVIQSFSPVICAVARIEAPEM
                     RVEFLAGAEPDKHEDWARIERWAFLLDLHGVNISYGSLTSGRVAVVRESGKTMAVWTV
                     DDLEQMRHCTSLGVDAIITNVPSRCLKLLGRASSAQRK"
     gene            6770..6928
                     /locus_tag="PKN92_05020"
     CDS             6770..6928
                     /locus_tag="PKN92_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48185.1"
                     /translation="MQHVSPAAGFVKSGFFSVFSGYACACAYSREVPAPGVNAARLRV
                     SRPGRQLL"
     gene            7135..8664
                     /locus_tag="PKN92_05025"
     CDS             7135..8664
                     /locus_tag="PKN92_05025"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNZ48186.1"
                     /translation="MKDTIYIFDTTLRDGEQSPGASMDENAKVQMALQLDRLGVDVIE
                     AGFPIASKQEFEGVIRVAKAVRNARVAGLARALSKDIERAAAALNYAQKPVLHTFLAT
                     SDIHMEYKLKMSRAQVIDTVGTAVTLAKSLLPRVEFSAEDATRSDRDFLAEVVETAIA
                     AGADVINLPDTVGYTTPDEIREMFRYVIDKAKGSEKVIFSTHNHNDLGLAVANALAAL
                     QGGARQVECTVNGIGERAGNTSLEEVVMAIHTRADEFPYHCGVETKEIVPSSNLLSMI
                     TGLVVPFNKPIVGRNAFAHESGIHQHGMLANSKTYEIMTPASVGHKRTDLVLGKHSGR
                     AGLAKRSAELGFNLNEEEVGKLYERFIVLADKKKEVYSDDLRMLIVAMYSNAFEVYQL
                     EKLRTFGEDPTVAMVKLKHGDEVITETALGDGPVDAACVAIEKIVGHTGKLEQFNLRA
                     ATPGKDAFGEAHVTVRFDDKVYRGNGASTDIVEAAIHAYLNAMNKHIAYLQSREINGD
                     S"
     gene            8939..9949
                     /locus_tag="PKN92_05030"
     CDS             8939..9949
                     /locus_tag="PKN92_05030"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNZ48187.1"
                     /translation="MVRSLVIRQAFRVLDITLTLAVIAVVVVVLRMFLTPLLPLEPGV
                     AVEDAGSVELATVLKTVGDRRSYDSLVKGGLFGNASDWDPAAAPPVEEPPAVEESAAI
                     EESELGLALRGTIALESGKEFAAALIENTEKREKPHPYKIDQEVLEGIVLVDIAQREV
                     ILLNKRQEPYRKERLSMQGGELQLTGGTGARPRGAAVASASGEAVYDQGSSRGNQARG
                     QGSTGASSSSSVQRMAVKRDEIIREAIQNYSSLATITPEVKTDPSGKVLGLTAENIEA
                     QPLAKKLGFRDGDVLQSVNNEEITSREQLYDLFQRYQTATSFRVGILRDGRPMVLNFQ
                     VD"
     gene            10127..12826
                     /gene="gspD"
                     /locus_tag="PKN92_05035"
     CDS             10127..12826
                     /gene="gspD"
                     /locus_tag="PKN92_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNZ48188.1"
                     /translation="MLNKNTVEGTLVYIHCSGFRCFAAVLLLLAGAGLAYAQYPDEYG
                     EAPLVEPPDGYDANGAVPDAPYTAAPPGAEYVDESGLETFNPGMRNAQQPPAVQPHSS
                     RPLSQGATPPPPPPIRDRVRPNRSGSAAPVASRAPLNSTTVSAQGNGSGNGNGQGNPM
                     ASEEKTGETPSDPVSFDFQDAPLLDVIAAISKLTGRNFDLDANLSSLTVSIITHDKIP
                     PEMAYEVLESILTTRGYSMVESLDGHLIKIIPTADAPTSDKTPLVLEKGAEIKGYDGF
                     STHIVTVENGNAEEIQRVLQLLGSKNARIDVYAPTNTLIITDTADGLRRMLAFLEQAD
                     VPGFDTTMEIFTLEYTRAEVISGQINQVLLGDTGQPQRGNMPPQPVPPTPVRPMHAPH
                     PAAVQQPGSQVIGSREEVLRMVSDERLNSLIVVATASMMERVRDLVKRLDTPTPYEAN
                     NMHIYQLVNADAEAIETAIQPLIGTAPRKQATAGGAAGGAAGMPSVGGGGGGAVGTPE
                     VQPFEQKVQITRYDRTNSLLIVASPQDYKLLEAFIARLDVPQRQVCVDAVIMEVKMTN
                     DFGLSVDAAAITGNDGFAMTSTDNLNGLLKTVHVAEEIVGGPRAALRGAALGLGSEQR
                     SGLTLGVYDDLSFTYRGQKIKIPFVPLLFQAVEKITDTEVLSQPSLVTMDNEESSIIV
                     GQEVPFITSTSSSRRTDGSVDSGMYGGYTRVERQDVGVKLKVTPQISEGDNVMVDVEI
                     EISDTNATPVGTVDIVGPTTNKSQVTNKTIVKDGSTAVIAGLIRDTAVKKHQPKAPIL
                     ADVPVFGWLFRSKSTSRQKQNMVVLVTPHIIKESMDLERISHSKIDEYYDSTVEQIVQ
                     AGFFDKILKKRSLSNDDSPTLKRAETMSGRRSSKNFKRGDIER"
     gene            12823..14559
                     /gene="gspE"
                     /locus_tag="PKN92_05040"
     CDS             12823..14559
                     /gene="gspE"
                     /locus_tag="PKN92_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNZ48189.1"
                     /translation="MSMQAATGLKLGEILAEQGWVHADQVKEALELQKLRPRRVGELL
                     LDLGYIEEENLLRALGLQFGLDYEPSVRKQVESSLTTKVPISFIREYHMVPYKRSNDG
                     YLVAINDPINLLPLDDLRLLLDGAVIPVLCSKEDIQFIIDTYFEQQSENAGELIDNIT
                     LSGEEEGKVHSLDHSESERDLLDLANEAPIIKLINLVISGAVKERASDIHLEPFEHSV
                     RVRYRIDGVLYEKFTVPRAQQAALVSRVKIMANLNIAEHRLPQDGRIKIRLSGKEIDI
                     RVSIIPIAHGERVVMRILEKGSFLFSLTQLGMDSRDHKIMDRLITSSHGIILVTGPTG
                     SGKSTTLYAALQQVNSPDKNIITVEDPIEYLIPGISQIQVRPKIGLTFAAGLRSILRQ
                     DPDIILVGEIRDMETAEMAVQASLTGHLVFATLHTNDSSGAITRLINMGIEPFLVTSS
                     TIAIQAQRLVRRVCPSCKENFMPDLDSLHELGISPDDPRCKELKRGVGCDKCSNRGYL
                     GRSGIFELLAMSNRIQEMVLQGADSNAVKREARLEGMRTLREDGAQKVLRGETTVEEI
                     LRVTRDETLESA"
     gene            14568..15797
                     /gene="gspF"
                     /locus_tag="PKN92_05045"
     CDS             14568..15797
                     /gene="gspF"
                     /locus_tag="PKN92_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HNZ48190.1"
                     /translation="MALYQYKAIIKSTGKTTRGVIDADGIVQARRKLREQNLYPTEVT
                     EDTSGGKGAGISGRVSNRGRVSGRDIALMTRQLAVLLRAGMSLLEALNALIDQTSRMR
                     LRAAIYEIRDKVNSGQRLGDALADHPRIFSDLYVNMVRAGEISGTLEQVLLRLADILE
                     RQHKLRSQIISTLAYPGFMGFFSVGIVVFLVMVIVPRITRMFERQETELPALTQGLIA
                     FSEFTSHYWWLILLALGAGFTLFRIWLSRPEGRRKWDRLKLKFPLYGQLHLKLVCARF
                     TRTMGTMLQSGLMMLPALDVVNAILGNTYIQERMDDVKVGVRRGRDLAAPLKESALFP
                     PMMIHMIELGQRSGEMENMLIQVADTFDEDVRLTIDALVALIEPIIIIFMGLVVAVLV
                     LAILLPIFQMSTSIGSS"
     gene            15868..16278
                     /locus_tag="PKN92_05050"
     CDS             15868..16278
                     /locus_tag="PKN92_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNZ48191.1"
                     /translation="MMKKNQETTRKQRQYQGGFTLIELMVVVAIIAILATTAGIYLFG
                     ALDDADQAKAQSEIKALKAAVTAYMLKNNRRLPESLEQVAEFMDPPKVPQDPWGNPYI
                     YTKDGARKYTIVSYGADGTPGGTGVNEDISSDKF"
     gene            16278..17195
                     /locus_tag="PKN92_05055"
     CDS             16278..17195
                     /locus_tag="PKN92_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48192.1"
                     /translation="MLLSADWFLADLMPERSVFPERIAVASFSGSISAKNGAGGKNGF
                     TLIELCVIILIIGTVAALALPQFMPLLLFSEVDGEARQLAQYGTAIVAEAALFGNDMT
                     VYIDLGGQVYYTMQTIYPDAADESGEAVDYLSMFNDFRKSGQHSSAELKEMLNGATQG
                     NLRLAGSLPADFDTTKANEQMAKEFDQRHNQLIYKRALNVKQNESFLSEIGPLFDKEF
                     KLSWSEPYEEELSLPLLARHNMPPAVRITSVAVGAKQYSSGLVAVNVTPLGLQYPVSF
                     QVCNDSGLCYTVDWNAVTGLGQAREAGAS"
     gene            17192..17626
                     /locus_tag="PKN92_05060"
     CDS             17192..17626
                     /locus_tag="PKN92_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ48193.1"
                     /translation="MKQTPFLPSRGAAGFTLVEVLVALAVLGGALFVLINAHYTALHL
                     HLMTQESVDERMLLESTVARAEMGVCQDELSGSGDFGPRYPGFSWSYEAAPTGDTEHP
                     LLADTRFFRVTVTLTRPDAEAKTLEFLTFVNEETKSVRVGAP"
     gene            17623..18429
                     /locus_tag="PKN92_05065"
     CDS             17623..18429
                     /locus_tag="PKN92_05065"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ48194.1"
                     /translation="MISVSGKKAGFTLLELLIAVTVLVVIVGLVYGSFSSVSLTMTLA
                     RADAERLRFRQILWRSFSGNLQSIYADAACLQEELQFLGENKDGGFGPADQLRFVSAL
                     PMPGATALPGILRVVSYTVTSRAETDSAIAAALPVTGSGSDTILLIREEPYQLQSQGF
                     YNDARSASGEVFEQAVPIASMNIQYFDPSKKEWQDQWNSLEERRIPGGIWIRINFART
                     EEDRQADYAAGIDLNEHPDFEFMLSFPMGRNVEYPFPDFNHLRYFSDEVL"
     gene            18452..19654
                     /locus_tag="PKN92_05070"
     CDS             18452..19654
                     /locus_tag="PKN92_05070"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNZ48195.1"
                     /translation="MNQSRTAQSGVALLLALVFIALMSALVFAFLFEMQVDASFAQNQ
                     GADFEALLAARSAVVQGIQILAEQYADVQDTGMPPADSEVDGSQWALGVPFEPLNDAT
                     MHASIDDEYGKINLNALILYDNGAPEPNEPLIQALRDFFAERSDSAYDPVDAILDWID
                     YEDGDAAEPDGAESDYYLSLETPYPCKNGPMDSIEELLLIKGITPELYFGNAEEKSGS
                     LPQEPLSEYLTVHGDWQGRVNVNTAREEVITAVIAGYTGTADPALGQQIYQEARVAPM
                     ENASQLRPYIPMADGGQRKGRVPDRNTGEAPEGVATDQEFRRAQRLQANDSVDEMFRY
                     NSSVFRIRGDGKVDDVLVRVEAFVFREPYDPREIEEPLSSLGLQYQQEFTDAPRQLFR
                     ILDWKVVQ"
     gene            19736..21175
                     /gene="pilM"
                     /locus_tag="PKN92_05075"
     CDS             19736..21175
                     /gene="pilM"
                     /locus_tag="PKN92_05075"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNZ48196.1"
                     /translation="MKVTAKIGAIEFVGNEVRVAMVKTGRSLPQVLDLQTRTAVYEAP
                     EDRVPAFARALNDALDALRSNPATYVLCVPCNMTVVRALDIPFKGLARVSKAVPFEIE
                     PHLAFPLEELLLDFNVIRESGSTTEVLAVGARKNHLEEQLAILQLAGVEAELAGVDVA
                     GLTSLWLATQKNVKGLRAVLHVRDDSSMLAITHNRALAYFRHLYFGVDALQTDPRRAA
                     REIQNTLRAFMAKWRSDMEFSSLAVTGCSLTVDETEVFEEILSMPVTTTVLMDALKKS
                     RSCTVDRSANFWEAAIGVGFSAGGGGLGFNLMKEAQQAQGAIRAVVAHLMFTACLALL
                     FLLGCAWYYHEGRLRNETLIEQAHDELNFLEGEIEKMAAEGLGADVKVSVFSDPTLLD
                     LLKELTEKLPRGSFHISEIKVNPPDARGGWINIAGSANSAAEFNTAFERLKESSMFRF
                     TEDTNIRLQGEKTTFRLRAFRPEDEIDEL"
     gene            21165..21689
                     /locus_tag="PKN92_05080"
     CDS             21165..21689
                     /locus_tag="PKN92_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48197.1"
                     /translation="MNYKLDLREKIGLSLGILACMLTFVLAVYVPSGPRKWYAQSQRE
                     LASLKEQLELQQLFYLDEAERLNKQKVLMEILANREPAFSLFTYVDNLLTSTGLRASA
                     QLEEYKPRDASPRQPMVQLRLQGVAFEQLVDFLHQIYSGNRLIALYKLDFLRPSPTEK
                     GLDCDITLVTLVQQ"
     gene            22050..23372
                     /locus_tag="PKN92_05085"
     CDS             22050..23372
                     /locus_tag="PKN92_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNZ48198.1"
                     /translation="MSFRTLLIAALIVSLGGVAFAELQNVEIGGSIRIRGNYFNLDSI
                     DRASFIEQRTRLNVRADFTQEVSAFIELDSYDYWGEDFRSWYLCGNDFRGGCDVGLYQ
                     AYIEARNLWGTPLSLRVGRQEIALGNQFLVGVNDTSSFFSGLSFDAVRLTFANEVVNI
                     DAIAAKLAENFGDFAEDDVDFYTVYGSYIGIEDITLDAYWMYVRDRAGVITRLANGQE
                     ADMHTVGLRGAGVIGGFDFEVEGAYQFGDLDGVPSACPLGFGEADVDFDAFAVNAEVG
                     YTFDMSLQPRLFARFAYLGGGKPDNNWWSNDRNLAFSRMFSNVQYSEFLDDWTFDNGA
                     MSNALVYTLGVQGQVTESFGLKLIGSYLTANEKQSRRARSKSLGWEVGLYADYNYSED
                     LVFRAGYAHLFGKKGLEPATIRWSGLAPWQGDRKDDYDYAFIETEICF"
     gene            23674..>24586
                     /locus_tag="PKN92_05090"
     CDS             23674..>24586
                     /locus_tag="PKN92_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNZ48199.1"
                     /translation="MKRKGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQNN
                     LKQWGLVFKMYANESPGEKFPTVQAGGFPWIDDNGNVIQAQLLVFDLGPNTFAIYPEY
                     LTDPNIMLCPSDPELGQHKKDIIDNTGNLNLSGVSAPSKYASAIDASYSYLGWCFDHY
                     DYEEPGLSVQPILQIMQIVGEASKIPANASSFSGPSQLIAGVEALLTKLSPLYLANNI
                     NGIAQLVDKDIELEASHAGLGNGQSNTIYRFREGIERFLITDINNPAASAQAQSVLPV
                     MFDHVAVALNLFNHVPGGSNVLYMDGHV"
BASE COUNT         5516 a         6136 c         7067 g         5867 t
ORIGIN      
        1 cactggatgc gctggaaagc ctggagggag actgcaccga atataccgtt ctttttgtgg
       61 gtcttgccgc cgcagccggt atcccggcga aaatctgtgc agggcttatt tatgtggaca
      121 gcgccaagtc aggtttttat tttcatcagt gggcgtctgt gtggatgggg gaatggattg
      181 atgtggatcc cgcctttaac cagatccctg ttgacgtaac gcatataaag ctcgccgaag
      241 gcgatctgtt ccggcaggca agaatccttc ccctcatcgg gcggctcagt atcactgtga
      301 atgaagaagc accccgccag gacgatcttt ccgaacccga agagcatgcc ggcgaagagc
      361 cgccccagga ataatcaggt ggcccgggcc tgattggtgg ggatccgaca gcttctggag
      421 catcgcagcc gtgtccgaac aaaaacagat ttccaacgat ggcagcgccg aatctcccgg
      481 ggcggtgtcg cccgtgccta cggaaaaagc acctgctcgc agaaaaagga aagtatttct
      541 gcttttcgtg ctgattctgc tgtctgtggt gctttttctc ggacagaccg gtctggtgga
      601 gcgtcagtgg ctgcctgccc tttcacaata tttcacggga tatgcacttc gttttgaacg
      661 catcgggctt tatccgctgg agctgcgcaa tctggagctg cgccggaagg gagattatga
      721 aaaagacccg ccgctgctgt ccgtctcctt tcttaagata gactggagcg tcacgtccct
      781 tttttcagaa aagcgactgt ttcgcgaaat ctcggcacat gggatcaggg cgatcattcg
      841 tgatagcggc gcgcttgcac tgcccggtga cggcgccgcc gccaaagaaa aaaaagcgtt
      901 tgcggcgccc gcttttttgc ttccccgggt gattcatttt gaagaccttt ctgtgcaggt
      961 acagaataaa gatacccgtg catcgctgga agggctgcgg ggtagtgctg tgatcaggag
     1021 tttttcagat ctgacagcag cagccggagg aacggaaata cgggcgcgct ggctgcccgg
     1081 attggaaaag gaagagctgt cgtttccgga aggaaatatt tctgttaagg cgcatcgggc
     1141 aggcgaagaa attcaggcgg aggcgcatct cagcataccg gacttgttgc agttccgtgc
     1201 tgaagcatcg ctgacagctg gcgaagctcc tgccttgcgt ctgaatatta tcgagtgcag
     1261 cgcacagggc aggctgcccg gtgagtttgt gtctccgttt cttcctgttc ctgtgttttt
     1321 cgagagactc gacgctgcac ctctgtctgt tactgccggg tggagcggtg gtgttccgga
     1381 agcacagcag ttatcttttg atctgcgctg taccgggctc acggtcggaa gagcggagtc
     1441 ccgtctttat gatggcgacc tgagcctgac tgcccggacg gcggcagaag gcgggggcga
     1501 aacagactgg acattgcagc tgaaccggaa acagcagatt tcaggtactc tccgggcgga
     1561 gtccggcacg gtgcatctca aagcggaagg agaaggttgg gacagggaag gggtgacggc
     1621 attgctgccc ggggcttacg caccttttgc atcgtggatt cctccgctga agaaggcgga
     1681 ttttgatctc gttgcccgta tgaattccgc cggagcggaa tttgaaggga atctgtcgcc
     1741 ccgtctggat tcaggtacgg agctgtccgc tcattttcag ggggcttatc agcatgccgg
     1801 ttcgggcgat atgacgatag aggcagttct gggcgacgga tcattgcagg gcagtgccgg
     1861 gctccatccc gggacgggta tatcggtgca ggcggcgtta aagcactgtt ctcttgccga
     1921 cctgctgtct ttcgttccgg cacagacagg ggctgaggcc tggtcgggga cgatggacgg
     1981 cagtcttgcg ttcagcaaat caccggggaa agaggccacg gtgaagacga cgctcactgt
     2041 cggaaacctg tttcatccgt cccttatgcc cgagaccctg gaggattctc tttcttttgt
     2101 tctgtcggca gtgctcagcg aagaccttgc ttctttacaa tcggcgcagt gtgatgtgga
     2161 agcaggggcg ttctttaagg cagccgtggg caagtgccgg ggcgatctga atacgggcgg
     2221 cctgcatttc tcttttcagg gagaggcgga tctgatggag gcaggcaccc gggcgggctt
     2281 cagtgactta tggggggcag tgcagtggga aacaacggtg gcagtgaaaa actggttgga
     2341 tatcgtgctg aatcccgcca caatcacttg tgaatctctt ggctggggag attattcatt
     2401 gccctacgga gaggtactga ctgtcacgtg tccggcccgc ctgtctttgc agaacaaccg
     2461 ggctgtgctt gggcccgttc ttatggagat aggcagtggt acttcgatgc gcgccgagga
     2521 aatcacgctc cggagtgatg cggtgaaagg aaaggggctt ttttctttca ccaccgattt
     2581 cgccccggtg aaagcgaagg gatggcttga agaggcggaa ggcaggctga atgcggagct
     2641 gcgggacttt gaatggatac cggaagggct gcagggcaac ctttcttttt cacttacacc
     2701 ttccagtttt gtgctgcctc ggtctcttgc ccggatgagc ggactggctg ttgacggaca
     2761 ggctcgactg ggcgcttctc cgacggcaga agctgatatc gttctggaag agctccttgc
     2821 cggaggcgtg aaaatcagtg cagcgcctgc ccgtctgact tttcaggagg ggaaggcaaa
     2881 agtagatccg ctgactttgg gcgtttttgg cggcaccctt aatctctcgc tgcaatggga
     2941 gccctttgct tcaggctttc cattgacggt acgcagcaac attgaccgca tagacctggg
     3001 cgtttttacc gacgaattta aaccccagtc cctcgtgctg acaggaattg tgcgcgggga
     3061 gatgttctgt gtgctcaccc cttcccagct ccgtgatttc agtctgatgc tggaatcaac
     3121 agaaaacttc agcatgaaca gggatatggt ggaacagttg ttgctcaacc agtacatgaa
     3181 tgatgtgacc ggttcccgtc aggtgagccg gatgctgcaa tcggtgctcg ggaaaaaacc
     3241 gcagaccgcc tttgatcggg cccaaatcaa actggggctt gaagagagcc gcattgctgg
     3301 aacggcactg ctggagagca agtcgctgaa tctcacggta gatatcaaag cggaccctcc
     3361 ggcactgctg gaagcgcttc gcataagaca ggaacacacg gattgagaat aacagggggg
     3421 tgaatatttg tcttataata aacaggatgc tgatcgggtg aataaacatt cgaatttcgg
     3481 cgtcctgtca acaccttcaa caggagaatg tcgcaatgag aaaaatagtg gctgttctgg
     3541 gagcagtggt tcttagtttc gcactgggtt gtgtaattcg tactgagcac aagatcaatg
     3601 ctcacattac actggatatc cgccatattg ccgatcaggc gacggatgtt ctggactatg
     3661 tggaagggaa atctgatgtg ctgccgggca tggagcctgc tcctgctcct gtgtcatcgt
     3721 ggtacgacag acttccatcg ttccgccttt ttgagacagc gtgggcagat tcattgagtc
     3781 agaccgattc gcctgtggtt cgtgagatcg caggaaaaat gcgggagcgc aatgccacca
     3841 ttgccgccct gaaaaaagag ggctgtctgg gcgaaaacaa ccggggatat gtggaacttc
     3901 gtgagtgcga tgcggcgaaa aacccggaaa agaaaaacga aattcagaaa acactggcag
     3961 aggaaaataa agaccgcaag gcgctttatg cggagatcgc ccggctcaac cgggaacttc
     4021 ccggcgtgaa tgtcaccaag gtggagcgga tttatgcgct ggagcgcatg cgtcgtgctc
     4081 aggcgggtga atcggttcag cttccacctg ccggagatga tttcaccgcc attcaaagtt
     4141 ctgaactcgg gaaggcactc ggagctgcct gtcagcccga cgcctgggtg atcattccct
     4201 gatcaggtct gtttcactca accaaaataa acagccgctc tcctgtacag acaagtcagg
     4261 agagcggttc tttttgtgct gcggcctggg ccggtgcgaa aaaaaaagag cggtgattca
     4321 ccggagtgaa ccaccgccac aagaagggtc gggggaagaa aaaggacgaa ttaccacgca
     4381 gctgcgatca gtccgatcac gtttgctgtg ttggaattga tgaaaccggt gccctgagcg
     4441 tcgttgccga tataccggat gaattcgacg tgtccgtcca tgtacagaac attgcatccg
     4501 cccggtacgt ggttgaagta ctggactgca gcaccggcac cgaacatatc aaacatgacc
     4561 tgcacggtgc tctgtgccat ggcgctggct gccgggttgt tgatgtcggt gatcaggaaa
     4621 cgttcaatac cttcacgcag acggtacaca gtgcttccgt tgccgttacc catgggaatg
     4681 gtgccgccgg gcgcaaagac attttcgata tccttatcca tggcctcgcg gctgccgctg
     4741 agaaagcgca tgagcacttc atccacagct ttaccgacct gaatgggaac gatggcactt
     4801 gtctgcacag ccgccggatt gggaagaatg cccagcaggg tcgtaatatt gggagcggag
     4861 tttgccagtg tatcacgcca ttcaggttta ttttccgctt tgtccataac atagccgaag
     4921 taggcatagc tttcatccat ggaccagcca tgctcatcca gataattgac atagtgagcg
     4981 aatacccagt cgccgttgga gtctttgagg ctgtcaacgg agttattgcc gtccgacggg
     5041 cagatcagaa ttgccggatc ggtcagatat tcggggtata cggctgagag ccgggggccg
     5101 atggcgatag cggcatcatg accgccggtc aggtccagcg ggttattgaa gtatgtggcg
     5161 gcgatgggag gaaacatctg acctttgtct tcattggcat acattttgta aatgagtccc
     5221 cactgcttca gattgttctg acaggaggag cggcgggctg cctcacgggc acgggcgagt
     5281 gcaggcagga ggatggcggc aaggatgccg atgatggcaa tgaccaccag aagttcaatc
     5341 agtgtaaagc ctcttctctt tttcatggtg taattccttt tactgcgggt tttgcggggt
     5401 gtcgtacacc gccgccgggc ataagggtat cagtacacaa gggacagtac gccctgtggc
     5461 gccgtccggg ctgtgcgaca ggacacgtat catgccgcca gcctgagctc tatggtaaac
     5521 gattggactg ttagaagtca aaccttaaaa tagatctaag ggggaaagtg ctctttttgc
     5581 agagaagaca tcttatgtca taatgccggg atgaatgttg atgcatggca gtctaaagga
     5641 gttatttcat gaaacatggt tctttctttt tgctttttgc tctgatcctg agcggatgcg
     5701 ccactttcag gcccggtgct ccgtccggta tgaccgatgt aattgcccat cgtggtgcga
     5761 gccatgatgc ccctgaaaat acattgcccg cctttgaact tgctattgag cagggggctg
     5821 actggtttga gcttgattgc cgtctgtcgc aggatggagc ggttgtggtg ttccacgacg
     5881 ataatctgga acgcatcaca ggactcgctg accgtgtgga cgccctggat tttgcatctc
     5941 tgcccacact ggatgccggc gcctggaaag ggccgtctta tgcgggtgct cagattccca
     6001 cactggcgga gtcgctggat cttgcgaagg ggcgtatcgg cgtttatgtg gaaatcaaaa
     6061 atgcggcaaa tgacggcgcc ctgctggaac agattcttgc catgtccgag ggtgtgcctg
     6121 tcatgacaga tgcccatgcc cggaaaattc ttgctgccat agaagaaagc ggcacaaaaa
     6181 atctggaact gacccgcaag acccttgcac tgattcggga acggcacatg gaaaaagaaa
     6241 tagtgattca atccttttcg cctgtgatct gtgctgttgc ccggattgaa gcgcctgaaa
     6301 tgcgtgtgga gtttctggca ggtgcggagc ccgacaaaca cgaggactgg gcgcgcattg
     6361 agcgatgggc ctttctgctg gatcttcacg gtgtgaatat cagctacggc agcctcacct
     6421 ccggcagggt tgcagtggtg cgtgaatccg gcaagacgat ggcggtgtgg acagtggatg
     6481 acctcgaaca gatgcgccat tgtacgtcac tgggtgtgga tgcgattatt accaatgtgc
     6541 cttcccgttg tctgaaactg ctgggccgag catcgtcagc gcagcgaaaa taaaggcgta
     6601 tgcggcagtg cctgctccgg aataagtggc agcattgcgc tgtttatcgg acaacaggct
     6661 gttcaactgt cttcatgcag ttctgttgtg ctatactttc tcaccttctc cgtactgcat
     6721 tgagcagaac agaagttctt tttccgcagg atctgtaaag gcaatcatta tgcaacatgt
     6781 atcaccggca gcgggctttg tgaaaagcgg attcttttcc gtcttttcag gctatgcctg
     6841 tgcctgtgca tacagcaggg aggtacctgc gcccggcgtt aacgcagcca gactgcgtgt
     6901 ttcccgtccg ggccgacagc tgctctgatt ccggacaggc ggaagacggc ggcaatcgag
     6961 tcagggcaat gttgtatcag gcgccggcag agatatttct gcgggtccat gacagacagg
     7021 cggatcggaa gctgcatccc attcttcccc ttgccggaat cgcgcaatgc acctgaatct
     7081 gttgtgtttt gctttctgca atggtttcca atttacaaaa aaggtagaag aataatgaag
     7141 gatacaatct atatttttga tacaacgctt cgtgatggag aacagtcccc cggtgcgagt
     7201 atggatgaaa acgccaaggt tcagatggcg cttcagctcg accgactggg cgtggatgtg
     7261 attgaggcag gctttcccat cgcttccaaa caggagtttg aaggggtgat ccgggttgcc
     7321 aaagcagtcc gcaatgcccg ggttgccggc cttgcccgtg cgttgagcaa agacattgaa
     7381 cgcgctgctg ctgcgctgaa ttatgcccaa aagcccgtat tgcacacctt tctcgccacc
     7441 tccgatatcc acatggaata taagctgaaa atgtcccgtg cccaggtaat cgatacggtg
     7501 ggaacagcgg ttacgctggc aaaatccctg ctgccccggg tcgaattttc tgccgaagat
     7561 gcgacccgct ccgaccgtga ttttctggcg gaagtggttg aaacggcaat cgccgccggt
     7621 gccgatgtga tcaatcttcc cgacaccgtc ggatacacga ccccggacga gatccgcgag
     7681 atgttccggt atgtgattga caaagccaaa ggctcggaaa aagtcatctt ttccactcat
     7741 aaccataatg atctggggct tgccgttgcc aatgctctgg cagcactgca gggcggtgcc
     7801 cgtcaggtcg aatgtacggt aaacggtatc ggtgagcggg cgggcaacac ttctctggaa
     7861 gaggtggtta tggcgatcca tacccgcgcc gacgagttcc cctatcattg tggcgtggaa
     7921 acaaaagaaa ttgtaccttc cagcaatctg ctgagcatga tcaccggtct ggtcgttccc
     7981 ttcaataaac ctattgtggg acgcaatgcc ttcgcacacg aatccggtat tcatcagcat
     8041 ggcatgctcg ccaacagcaa gacctatgaa attatgacgc ctgcttcggt gggtcataag
     8101 cgtacggatc tggtgctcgg caagcattcg ggccgtgccg gtctggcaaa acgcagtgcc
     8161 gaactcggct ttaatctgaa tgaagaggaa gtcggaaagc tgtacgagcg ttttattgtt
     8221 ttagccgaca agaaaaaaga agtctattcc gatgacctgc gcatgctgat cgttgccatg
     8281 tattccaatg catttgaagt gtatcagttg gagaaactgc ggactttcgg ggaagacccc
     8341 accgttgcca tggtcaagct gaaacatggt gatgaagtga ttacggaaac ggcgcttggt
     8401 gacgggcccg tggatgccgc ctgtgttgcc atcgaaaaaa tagtggggca tacaggaaaa
     8461 ctggaacagt tcaatctgcg cgccgctacg ccgggcaaag atgctttcgg tgaagcccat
     8521 gtgactgtac gctttgatga taaagtctac agggggaacg gcgccagtac agacattgta
     8581 gaagccgcca ttcatgccta tctgaatgct atgaacaaac atatagcata tctgcagagc
     8641 cgtgaaatta atggtgattc ctgacctttg cgtttgtgcg gacaaccggt aagataggga
     8701 ctcggaaaag ggatttctct tttccgagtc tttccatgaa agatgtcttg cgtaatctgc
     8761 ccgcttttct ctgtgttgca tgaaaggctt tgcggtataa tgttaaccgt gggtaagcgc
     8821 attccgttct ttgaacgtat gcgagtgact catctcccgg tctgattatt cttcattttc
     8881 gcctttgggc gagggaagag gtcgggcggt tttttgcttt cgtaattaaa ggatgcacat
     8941 ggtacgcagt ttagtcatac ggcaggcttt ccgggtcttg gatatcaccc ttaccctggc
     9001 ggtaatcgct gtggtagtgg ttgttctccg catgtttctg acaccgctgt tgccactgga
     9061 gccaggtgtt gccgtggaag atgcgggctc cgtggagctg gcgactgtat tgaagactgt
     9121 tggggatcgc cgctcttatg acagcctggt taaaggcggt ctcttcggca atgccagcga
     9181 ctgggatcct gcggctgccc ctccggtcga agagcctcct gccgttgagg aaagtgccgc
     9241 aatagaggaa agtgaactcg ggctcgccct gcggggcacc attgctcttg agtcgggcaa
     9301 ggagtttgcc gctgctctta ttgagaacac tgaaaaaaga gaaaaacctc atccctacaa
     9361 aatagatcag gaagtgctgg agggcatcgt cctagttgac attgcccagc gggaagttat
     9421 tctgctgaac aaacggcagg aaccttaccg aaaagaacgg ttgagtatgc agggcggcga
     9481 actgcagctg accgggggta caggcgcacg ccccagaggt gcggcggtgg cttccgcttc
     9541 aggagaagcg gtttatgatc agggaagttc gagagggaat caggcgcgcg ggcagggcag
     9601 cacgggtgct tcttcctctt cttctgtgca gcgcatggct gtcaagcgtg acgaaattat
     9661 ccgtgaggcg atacagaatt attcttcgct ggcaacgatc actccggaag tcaaaactga
     9721 cccgagcggc aaggtgctgg gccttactgc ggaaaatatt gaggctcagc cactggcaaa
     9781 aaaactgggt tttcgtgacg gtgatgtgct gcagtctgtg aataacgaag aaattaccag
     9841 ccgcgagcag ctttatgatt tatttcaacg ctatcagacc gccacttctt tccgtgtcgg
     9901 cattttaagg gacggcaggc cgatggtact caattttcag gtagactgat gctgccgggt
     9961 tttacagtgc tgcgacgctt cttttccctt atcgctgctc acagaaaaat cgatcccggt
    10021 ctggttgata aaatgggcat cccgggtagg gcatgcgtta taatgcatgc gagttttgac
    10081 aacggtgttc aggagcactt ttgctgcagg atttcttttt aatcccttgc tgaataaaaa
    10141 tactgtggaa gggacgcttg tgtatataca ttgttctggt ttccgctgct ttgcggcagt
    10201 tcttctttta ttagctggtg ccggtcttgc ttatgcccag tatcctgacg agtacggtga
    10261 ggctccgctt gtagagccgc cggacggtta cgatgcgaac ggtgccgtgc cggacgcgcc
    10321 ttataccgca gcgccgccag gcgccgagta tgtggacgag tccggcctgg aaaccttcaa
    10381 tccgggcatg cgaaatgccc agcagccgcc tgctgtgcag cctcattctt cccgcccgct
    10441 gtcgcagggc gcaacgccgc cgccgccacc gcccatccgt gatcgtgttc gccccaacag
    10501 gagcggatct gcggcaccgg tcgcttcccg tgcacccttg aacagtacca cggtcagtgc
    10561 tcaggggaac gggagcggca atggcaatgg ccagggtaat cccatggcat cagaagaaaa
    10621 gacaggcgaa acgccgtctg atccggtcag ctttgatttt caggatgcgc cgctgctgga
    10681 tgtgatagcc gccatctcca aactgaccgg gcgcaatttt gatcttgatg ccaatctttc
    10741 atccctcaca gtttccatca tcactcatga taaaattccg cccgaaatgg cgtatgaagt
    10801 gctcgaatcc attctgacaa cccgcggtta ttccatggta gaaagtctgg acgggcacct
    10861 gatcaagatt atccccactg ctgatgcgcc cacttcagat aaaacaccgc tcgttctgga
    10921 aaaaggggct gaaatcaagg gctacgacgg cttctccacc cacattgtca ccgtggaaaa
    10981 cggcaacgcc gaggagatcc agcgggtact gcagctgctg ggttccaaaa atgcgcgcat
    11041 cgacgtatat gcgcccacca atacgctcat tattacggat acagcggacg gtcttcgccg
    11101 catgctggct ttcctcgagc aggcggatgt gcccggcttt gatactacca tggagatttt
    11161 tacgctcgag tatacccgtg ccgaagtgat ctccggtcag atcaatcagg tgctgctggg
    11221 tgataccggc cagccgcagc gcggcaacat gcctccgcag cccgttccgc cgacaccggt
    11281 acggcccatg catgctcccc atccggcagc ggtgcagcag ccgggttcgc aggtgatcgg
    11341 ttcccgggaa gaagtgctgc gcatggtcag tgatgagcgt ctgaactcct tgattgttgt
    11401 ggcgacggca tcgatgatgg aacgggtgcg cgatctggta aaacgccttg acacgccgac
    11461 gccctatgaa gccaacaaca tgcatattta tcagcttgtc aatgccgatg ccgaggcaat
    11521 tgaaacagcc attcagcctc ttatcggtac agcaccgcgt aagcaggcaa ccgcaggcgg
    11581 tgcagcaggc ggtgcggcag gcatgccctc ggtcggcggc ggaggcggcg gtgccgtcgg
    11641 aacgcctgag gtgcagcctt tcgagcagaa ggtgcagatt acccgctatg acagaacaaa
    11701 ttcgctgctg attgtcgctt ccccgcagga ttacaaactg cttgaagcgt ttatagcccg
    11761 gctggatgtg ccgcagcgtc aggtgtgcgt ggatgcggtc atcatggaag tgaagatgac
    11821 caatgacttc gggctttctg tcgatgctgc cgctattacc ggcaatgacg ggtttgccat
    11881 gaccagcacg gacaacctga acggcttgct gaagaccgtg catgtggcgg aagaaattgt
    11941 gggcgggccc cgtgccgcct tacgcggagc cgccctcggg ctgggcagcg agcagcgcag
    12001 cggtctgacc ttgggtgttt atgatgatct gagcttcact tatcgtggtc agaaaatcaa
    12061 gattcctttt gtgccgctcc tctttcaggc tgttgaaaaa atcaccgata cagaggtgct
    12121 ttcacagccc agtctggtga ccatggacaa cgaggaatca agtattatcg tcggtcagga
    12181 agtgcctttt attaccagca ccagctcctc ccgtcgcacg gatggttctg tggattcagg
    12241 tatgtacggc ggatacaccc gggtcgaacg ccaggacgtg ggtgtgaagc tgaaagtgac
    12301 accgcagatt tccgaaggag acaatgtcat ggttgatgtt gagattgaaa tctcagacac
    12361 caacgcgact cctgtcggaa cagttgatat cgtcgggccg acaaccaata aatcacaggt
    12421 caccaacaag accatagtca aggacgggtc gacagcggta attgcagggc ttatacgtga
    12481 cacagcggtg aaaaagcatc agcccaaggc gcccattctt gcggatgtgc ctgttttcgg
    12541 atggcttttc cgctccaaat ccacctcgcg gcagaagcag aacatggtag tgcttgtaac
    12601 gcctcatatc atcaaggaaa gcatggatct ggaaagaatc agccatagca aaattgatga
    12661 atactacgat tcgactgtgg agcagattgt gcaggccggt ttctttgaca agattttgaa
    12721 aaaaaggagc ctttccaacg atgacagtcc tacgctgaag cgtgctgaaa ccatgagtgg
    12781 caggcgcagc tccaaaaatt ttaagcgcgg cgacattgaa agatgagtat gcaggctgcc
    12841 acgggattaa aactgggtga aatactggcg gaacagggct gggtacatgc cgatcaggtg
    12901 aaggaagcac tggagctgca gaagctgcgc ccccgtcggg taggggaact tttactggat
    12961 ctgggttata tcgaggaaga aaatctcctt cgcgctctgg ggctccagtt cggcctggat
    13021 tatgaaccgt cagtgcgaaa gcaggtcgag tcgtcgctga ccacgaaggt acccatcagc
    13081 tttatccgtg aataccacat ggtaccctat aaacgcagca atgacgggta tctggttgca
    13141 atcaacgatc cgatcaatct gctccctctg gatgatctcc gcctgctgct ggacggcgct
    13201 gtcattcctg tgctctgcag caaggaagat attcagttca tcatcgacac gtattttgag
    13261 cagcagagcg aaaacgccgg agagctcatc gacaatatca cgctgagcgg ggaggaagag
    13321 ggcaaggttc actcgctgga tcacagcgaa tccgagcggg atcttcttga tcttgccaat
    13381 gaagccccga tcatcaaact gatcaatctg gtgatttccg gtgccgtgaa agaacgggct
    13441 tcggatatcc atctggagcc ttttgaacat tcggtgcggg ttcgttaccg tatcgacggg
    13501 gtgctctacg agaaatttac agtgccccgg gcacagcagg cggcgctggt gtcccgcgtg
    13561 aaaattatgg cgaacctgaa tatagccgag catcgcctgc ctcaggacgg ccgtatcaaa
    13621 atacggctga gcggaaaaga aatcgatatt cgtgtatcta ttatccctat tgcccatggc
    13681 gagcgggtgg tcatgcgtat tctggagaag ggcagttttc ttttcagcct gacccagctg
    13741 ggcatggaca gtcgtgatca taaaattatg gatcggctga tcaccagttc tcacgggatc
    13801 attctcgtga caggacccac gggctcggga aaatcgacga ccctctatgc cgccctgcag
    13861 caggtgaact cgccggataa aaatatcatt accgtggaag atcccattga atacctcatc
    13921 ccggggatca gccagattca ggtcaggccc aaaatcggac tgacttttgc cgccggtttg
    13981 cgcagtattt tgcggcagga cccggatatt attctggtcg gcgagatccg tgacatggaa
    14041 acggcggaaa tggcggtgca ggcttctctt acggggcatc tggtttttgc caccctgcat
    14101 accaatgaca gttcaggtgc cattacccgc ctgatcaata tgggcataga gccctttctc
    14161 gttacgtctt ccaccattgc aattcaggcg cagcgcctgg tgcgccgggt ctgcccttcc
    14221 tgcaaggaaa acttcatgcc cgatctggac tcgctgcatg aactgggtat ttcacccgat
    14281 gatccgcgct gtaaagaact gaaacggggg gtcggctgcg acaaatgcag caaccggggc
    14341 tatctgggca gaagcggcat ttttgagctc ctcgccatgt ccaacaggat tcaggaaatg
    14401 gtgctgcagg gtgcggactc caatgcggtg aagcgggaag cgcgtttgga aggtatgcgc
    14461 acactccgtg aggacggtgc gcagaaagta ctgcgcggcg agacaacggt agaagagatc
    14521 ctgcgggtta cccgcgatga aacgcttgaa tccgcatagg tatttttatg gcactttatc
    14581 agtataaagc gattattaaa agtaccggca agacgacacg cggtgtaatc gatgccgacg
    14641 gtattgtgca ggcacgccgc aagctgcggg agcagaatct gtaccccacc gaagtaacgg
    14701 aagatacttc cggaggcaag ggcgccggta tcagcggacg cgtttccaat aggggcaggg
    14761 tctcggggcg ggatattgcg ctgatgaccc gtcagttggc ggtgctgctc cgggcgggca
    14821 tgtcgctgct ggaagcgctg aatgcgctga tcgatcagac aagtcgcatg cgacttcggg
    14881 cggcgattta tgagattcgg gacaaggtga acagcgggca gcgtctgggc gatgcgcttg
    14941 ctgaccatcc gcgtattttt tcggatctgt atgtgaatat ggtgcgtgcc ggagaaatca
    15001 gcggcaccct ggagcaggta cttctacggc tggcagatat tctggagcgc cagcataagc
    15061 ttcgttcaca gatcatttcc accctggcat accccggatt catgggattt ttttcagtgg
    15121 ggattgttgt ctttctggtg atggtgatcg ttccacgaat aacacgtatg tttgagcgtc
    15181 aggaaacgga actgcccgcc cttacgcaag ggcttattgc tttcagtgag tttacaagtc
    15241 actactggtg gctgattctg ctggcgcttg gcgccggctt caccctgttc cgtatctggc
    15301 tttccagacc cgagggccgc cggaaatggg accggctgaa gttgaaattt ccgctgtacg
    15361 gacagcttca tttgaaactg gtttgcgccc gattcacgcg caccatgggt accatgcttc
    15421 aaagcggact tatgatgctt ccggcactgg acgtggtgaa tgccattctg ggcaatacct
    15481 atattcagga acgcatggat gatgtgaaag taggtgtgcg tcgcggacgt gatctggctg
    15541 cgccgctgaa agaatccgcg ctgtttccgc ccatgatgat tcatatgatt gaactgggtc
    15601 agcgaagcgg cgagatggaa aatatgctga tacaggtggc ggatactttt gatgaggatg
    15661 tgcgccttac cattgatgcg ctggtggcgc tgatcgagcc catcattatc attttcatgg
    15721 ggcttgttgt ggcggtgctg gtactcgcca ttctgcttcc tattttccag atgagcacaa
    15781 gcattggttc cagttaagcc aaacagtccg gcatctcgtt ttttctggag aaccgacttt
    15841 cagtcagggc acagcccggg agatattatg atgaaaaaaa accaggagac aacacgaaag
    15901 cagcggcagt atcagggcgg tttcactctg attgaactga tggtggttgt ggcaattatt
    15961 gcgatccttg ccacgacagc gggtatttac ctctttggtg cgctggacga tgcggatcag
    16021 gcaaaggcac aatcggaaat caaagcgctt aaagcggcgg tcactgccta tatgctgaaa
    16081 aacaaccggc gcctgccgga atcgcttgaa caggtcgccg agtttatgga tccgcccaaa
    16141 gttccgcagg atccctgggg caacccttat atctatacca aagacggcgc acggaaatat
    16201 acaatcgtat cctacggcgc tgacggaaca cccggcggaa ccggggtgaa cgaagacatc
    16261 tccagcgaca agttctgatg cttctttctg ctgactggtt tttagcggat ttgatgccgg
    16321 aaagaagtgt gtttccggag cgcatcgctg ttgcctcttt ttccggcagc atatccgcga
    16381 aaaacggtgc cggcggaaaa aacggtttta cactcataga actctgtgtc atcattctga
    16441 ttatcggtac cgttgcagcg ctggcactgc cccaatttat gccccttctg cttttttccg
    16501 aagtggacgg agaagcgcgt cagctggcgc agtacggcac cgccattgtt gctgaagccg
    16561 ctcttttcgg aaatgacatg accgtttata tcgatctcgg cgggcaggtc tattacacga
    16621 tgcagaccat ttatccggat gccgccgacg aatcaggcga agccgtcgat tacctttcca
    16681 tgttcaatga tttccgaaag tcgggccagc attccagtgc cgagttgaaa gagatgctca
    16741 acggcgccac ccagggtaac ctcaggcttg ccggcagcct tccggcggac ttcgacacta
    16801 cgaaagccaa tgaacagatg gcgaaggaat ttgatcagcg tcataaccaa ctgatttaca
    16861 agcgtgcgct caatgtaaag cagaacgaga gtttcctcag tgaaatcggg ccgctttttg
    16921 acaaagagtt caaactatcc tggagcgaac cctatgaaga ggaactatcc ctgcctcttc
    16981 tggcacggca caacatgcct ccggctgtcc ggatcacctc tgttgcagtt ggtgcaaaac
    17041 aatattcgag cggcctggtt gccgtgaatg tgacccctct ggggctgcaa tatcctgtct
    17101 cttttcaggt gtgtaacgat tccgggcttt gctatacagt ggactggaat gctgttaccg
    17161 ggctgggtca ggcacgggag gcgggcgctt catgaagcag acaccttttc tcccctctcg
    17221 tggtgcagca ggcttcactc tggtggaagt cctcgtggcg ctggcggtgc tgggtggtgc
    17281 tctgtttgtg ctgattaacg cccactatac agccctgcac ctgcatttga tgacacagga
    17341 gtctgtggat gagcgcatgc ttctcgagag tactgttgcc cgtgctgaaa tgggcgtatg
    17401 ccaggatgag ctgagcggca gcggtgattt cggcccgcgc tatcccggtt tttcctggag
    17461 ttacgaggcg gcgccgaccg gagacacgga gcatccgctt ctggcggata cccgtttttt
    17521 tcgcgtgacg gtgaccctta cccgtcccga tgctgaggca aaaacacttg agtttctgac
    17581 cttcgtcaat gaagagacga aaagcgtccg ggtgggtgcg ccatgatcag cgtgtccggt
    17641 aagaaggcgg gtttcacgct gctggagctg ctcatcgctg tcacggtgct tgtagtgatt
    17701 gttgggctgg tctacgggag tttctcctcc gtttctctta ccatgacttt agcgcgtgcg
    17761 gatgcggaac gtctgcggtt ccggcagatt ctctggcgca gcttctccgg caatctgcag
    17821 agcatttacg ccgatgcggc ttgccttcag gaggagctgc agtttctggg ggaaaataaa
    17881 gatggcggct tcgggcccgc tgaccagctg cgttttgtca gtgcgttgcc gatgcccgga
    17941 gcgacagcac tgccgggcat tctgcgggtg gtctcttaca ctgtaaccag ccgggctgaa
    18001 acagacagcg ccattgcggc ggcgctgccg gtgaccggct ccggcagcga cactattttg
    18061 ttgattcgtg aggagcccta ccagctgcag tcgcagggtt tttacaatga tgcccgcagt
    18121 gcttcgggcg aagtctttga gcaggcggtg cccattgcat ccatgaatat ccagtacttt
    18181 gatccctcca aaaaagaatg gcaggatcag tggaattctc ttgaagagcg gcgtattcct
    18241 ggcggtatct ggatcagaat taattttgcg cgtactgaag aagaccggca ggcggactat
    18301 gctgccggca tcgatctgaa tgagcatccg gattttgagt ttatgttgtc ttttccaatg
    18361 gggcgcaatg tggaatatcc tttcccggat tttaatcacc tgcgctattt ttcagacgaa
    18421 gtgctttgat gtgaatacag cgacaccacc tatgaatcag tctcgaacag cgcaaagcgg
    18481 tgttgcgctt ttgcttgcac tggttttcat tgcgctgatg tcggcgctgg tttttgcttt
    18541 tctttttgaa atgcaggtgg atgcctcctt tgcgcagaat cagggagcgg actttgaagc
    18601 ccttctcgcg gcacggtctg ctgttgtgca gggaattcag attcttgccg agcagtatgc
    18661 cgatgtgcag gacacgggca tgccgccggc agacagcgaa gtggacgggt cacagtgggc
    18721 tctgggtgtg ccttttgagc ctttgaacga tgcaaccatg catgcctcca ttgatgatga
    18781 gtatggtaag attaacctga atgcgctgat tctttatgat aacggcgcac cggaacccaa
    18841 cgaaccgctg attcaggctt tgcgtgattt ttttgccgaa cgcagcgaca gtgcttatga
    18901 tcccgtagat gccattctcg actggatcga ttatgaggac ggcgatgcgg cggaacctga
    18961 cggcgctgag tcggattatt atctcagtct ggaaacgccg tatccctgta aaaacgggcc
    19021 catggattcc attgaagaac tgctgctgat caaaggcatt acaccggaac tctatttcgg
    19081 caatgcagaa gaaaaatccg gctcgcttcc gcaggaaccg ctctccgaat atctgacagt
    19141 gcacggtgac tggcaggggc gggtgaacgt caacacagcc cgggaagaag tgattacggc
    19201 tgtgattgcg gggtatacag ggacggctga ccccgcactg ggtcagcaga tttatcagga
    19261 agcgcgggtg gcgcccatgg aaaatgcgag tcagctgcgc ccgtacatcc cgatggcgga
    19321 cggcggtcag cggaagggtc gggtgccgga cagaaataca ggcgaagcac ctgaaggtgt
    19381 cgcaacggat caggaattcc gtcgtgcgca gcgcctgcag gcgaatgact cggttgatga
    19441 aatgttcaga tacaacagca gtgttttccg tatccggggt gacggcaaag tggatgatgt
    19501 gctggttcgg gtggaggctt tcgtattccg ggagccctat gatccgcgtg aaattgaaga
    19561 acctctctct tctctcggtc tgcagtatca gcaggaattt actgacgctc cccggcagct
    19621 cttccgtatt ttagattgga aagtagtaca ataaaagaaa gaaaccgaga acttctccgg
    19681 agcggcagaa agggctgtcg gttcgggaaa caccggtgct caacaggata tacacatgaa
    19741 ggtaacggca aaaatcggcg ccattgaatt cgtgggaaat gaggttcggg tcgccatggt
    19801 caagacgggg cggtcattgc cgcaggtgct ggatctgcaa acgcgcacgg cggtgtatga
    19861 agcgcctgaa gacagagtgc ccgcttttgc ccgggcgctg aatgatgcgc tggatgcgct
    19921 tcgatccaac ccggcgacct atgtgctctg tgttccctgc aatatgacgg ttgttcgtgc
    19981 actggatatt ccgtttaagg ggcttgcacg tgtgagcaaa gccgtgcctt ttgaaattga
    20041 gccgcacctc gcttttcctc tggaagagtt gctgctggat tttaatgtga tccgggaaag
    20101 cggaagcacc acggaggtgc ttgcggtagg tgcccggaaa aaccatctgg aagaacagct
    20161 ggcgattctt cagctggcgg gcgttgaggc ggagctcgcc ggagtggacg tagccggcct
    20221 gacttcgctg tggctggcga cgcagaaaaa tgtgaagggg ctcagggcgg tgctgcatgt
    20281 gcgtgacgac agctccatgc ttgccattac gcataacagg gcgcttgctt atttccggca
    20341 tctctatttt ggtgtggatg cactgcaaac tgacccccgc agggctgccc gtgaaataca
    20401 gaatacgctg cgcgctttca tggcaaaatg gcgcagcgat atggagtttt caagccttgc
    20461 cgtcaccggc tgttcactga cagttgatga gaccgaagta tttgaagaaa ttctgagtat
    20521 gcccgtaacc acaaccgttc tgatggacgc actgaagaaa agccgatcct gcacagtgga
    20581 ccgctccgcc aatttctggg aggctgccat tggtgtcggg ttcagtgccg gcggtggcgg
    20641 actgggattc aacctgatga aagaggcaca gcaggcacag ggtgctatac gtgccgttgt
    20701 ggcgcatctg atgtttaccg catgtctggc actgctgttt ctgcttggct gcgcctggta
    20761 ctatcacgag ggcaggctgc gcaacgaaac actgatcgag caggcacatg atgaattgaa
    20821 ttttcttgaa ggcgagatag aaaaaatggc agctgaaggg ctgggtgcag acgttaaagt
    20881 cagtgttttc agtgatccga cactgctgga tctgctgaaa gagctgactg aaaagctccc
    20941 gcgcggaagt tttcatatca gtgaaattaa agtaaatcct ccggacgccc ggggcggctg
    21001 gatcaatatt gccgggtcgg cgaacagtgc cgccgagttt aacaccgctt tcgagcgtct
    21061 gaaagaatca agcatgttcc gctttactga ggatacgaac atccggttgc agggagaaaa
    21121 gacaacattc cgtctccgtg ctttccgtcc tgaggacgaa atagatgaat tataaattgg
    21181 atcttcgtga aaaaattgga ctgtcgctgg gaattctggc gtgtatgctc acatttgttc
    21241 tggctgtata cgttccgtcg ggcccaagaa aatggtatgc ccaatcccag cgtgaactcg
    21301 cttcgttgaa agagcagctg gaattacagc agctgtttta tcttgatgag gcggaacggc
    21361 tgaataagca gaaagttctg atggaaatcc tggcaaatcg ggagcccgct ttttcacttt
    21421 ttacttatgt cgacaatctg ctgacctcga ccggtctgag agcgagtgcg cagctggaag
    21481 aatacaagcc ccgtgatgca tcgcccaggc agcccatggt gcagctgcgc ctgcagggag
    21541 tcgctttcga gcaattggtt gacttcctgc accaaattta ttcgggcaac cgccttatcg
    21601 ccctgtataa actggacttt ctgcggccat cgcctacgga aaaagggctg gactgcgata
    21661 tcaccctggt gactttggtg cagcaataac acaagtcagc cacaaagggt tgtactgaaa
    21721 caactcttcc agcagaaaac cactatttct tgtatgccct tgttttttca gggcgatttt
    21781 gctatacttt cagatatgaa gatggataaa taacatctgt acggattata aacgaaggaa
    21841 gtattgtttg aaggcatata tccctgcgca catattttta tgacatgaaa ggagggatga
    21901 gcaggagaaa ggcgatttac gtctggtcac ctgtgcgcgg cagcgataaa tatttatagc
    21961 agttgactga agtgcgtttt tgcagcggga aggatgctgc aaagcgcgca ggcttagctc
    22021 cacaacatta agtacaagga gatctgaata tgtcttttag gactttgctt attgccgctc
    22081 tgatcgtgag tctgggcgga gttgcttttg cggaactgca gaacgttgaa atcggcggca
    22141 gcatccgtat tcgcggtaac tattttaacc ttgacagcat tgatcgcgcc agttttattg
    22201 aacagcgcac ccgtctgaat gtccgtgctg attttacgca ggaagtcagt gcttttatcg
    22261 aactggacag ctatgattac tggggcgaag atttccgttc ctggtacctt tgcggtaatg
    22321 atttccgcgg tggctgtgat gtaggtctgt atcaggcata cattgaagca cggaatcttt
    22381 ggggcacacc cctgagcctt cgtgtgggcc gtcaggaaat tgcgctgggc aatcagttcc
    22441 tggtgggcgt caatgacact tcctccttct tctccggtct gtcttttgat gcggtgcgtc
    22501 tgacttttgc gaatgaggtc gttaacattg atgctattgc cgcgaaactt gccgaaaact
    22561 tcggtgactt tgcggaagac gatgttgatt tttacacggt ttacggcagc tacatcggca
    22621 ttgaagacat tacgcttgat gcttactgga tgtacgtccg cgaccgtgcc ggtgtgatta
    22681 cccgccttgc caatggtcag gaagcggata tgcacacggt cggcctgcgc ggtgccggtg
    22741 tgatcggcgg attcgacttt gaagttgaag gtgcctatca gttcggtgat ctggacggcg
    22801 ttccttccgc atgccccctg ggctttggtg aagccgacgt tgactttgat gcctttgccg
    22861 tcaatgcaga agtaggttac acctttgata tgtcgctgca gccccgcctg tttgcccgct
    22921 ttgcctatct tggcggcggc aagcccgaca acaactggtg gagcaatgac cgcaatcttg
    22981 ccttctcccg catgttctcc aacgttcagt acagtgaatt tctggatgac tggacgtttg
    23041 acaacggcgc catgtccaat gcgcttgttt acacactggg cgtgcaggga caggtcaccg
    23101 agtctttcgg tctgaagctg atcggatcct acctgacggc caacgagaag cagagccgcc
    23161 gcgcccgctc caaatctctg ggttgggaag tgggtcttta tgctgactac aactacagcg
    23221 aagacctggt attccgtgcc ggttatgccc acctcttcgg taaaaaaggt cttgaacctg
    23281 cgaccatccg ctggagcgga ctggcaccct ggcagggtga tcgtaaagac gattacgact
    23341 atgcctttat cgaaacggaa atttgcttct gatttaaatt tcattctctg ctgattttca
    23401 caccctcgaa gcacctgttg tttcgagggt gtatttattg ttttttagca taatttgttc
    23461 ttgtttctgc atctgttttt ttcggcaaac aagatcttga caatgccatt taaaagtggt
    23521 atacttgttc tgaacagtgt tcaggtttat tgacacagtt ataatttaat ctcgcgtttg
    23581 tcacataacc ggaaaacggc gtttcatgcc gtggggattg tatgcccctt ccggaagtgt
    23641 gatagggata catacctacg taggagtcat gcaatgaaac gcaaaggatt cacactcatt
    23701 gagctgctgg tggtaattgc cattatcggc attcttgcgg caattctgct gccggcactc
    23761 gcccgggcac gcgaagcggc acgccgctcc agctgccaga acaatctgaa gcagtgggga
    23821 ctggtattca agatgtatgc caatgaatcc cccggggaaa aattccctac agtccaggcc
    23881 ggcggatttc cgtggattga tgacaacggc aatgtgattc aagcccaact actggttttc
    23941 gacctgggcc ccaacacctt tgccatctat ccggaatatc tgacggaccc caacatcatg
    24001 ctgtgtccct cggatccgga actgggtcag cataaaaaag acatcatcga caatacaggc
    24061 aatctgaatc tgtccggcgt cagcgccccc agcaaatatg ccagtgccat tgatgccagt
    24121 tacagctatc tgggctggtg cttcgatcac tatgattatg aagagcccgg tctgagcgtt
    24181 cagccgatcc tgcagatcat gcagattgtg ggcgaagcca gcaagattcc ggcgaatgcc
    24241 tcctccttct ccggtcccag ccagctcatt gccggtgttg aagcactgct gaccaagctg
    24301 agccccctgt atcttgccaa caacatcaac ggcattgctc agcttgttga caaagatatc
    24361 gaacttgaag catctcatgc cggtttgggc aatggccaga gcaacaccat ttaccgcttc
    24421 cgtgaaggta tcgagcgctt cctgatcacc gacatcaaca acccggcggc cagtgcacag
    24481 gcgcagagcg ttcttccggt gatgttcgac cacgtcgctg ttgcgctgaa ccttttcaac
    24541 cacgttcccg gcggctccaa cgtgctttac atggatggtc acgttg
//
LOCUS       DAOPWB010000036        24496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2024_length_24496_cov_4.743259, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000036 DAOPWB010000000
VERSION     DAOPWB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 24496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24496
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2024_length_24496_cov_4.743259"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKN92_05095"
     CDS             complement(<1..352)
                     /locus_tag="PKN92_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48200.1"
                     /translation="MPLQLVNNRDIHIAQEWIIQLSALMQAPDTPGFDPFDIKAHPLF
                     KKAQQRPWLRKVTTVLGDAFPTVLRRMLRIDRSLNPKTPALFALGHLRLFQITGDGIH
                     LEKAKHCLELLRSLA"
     gene            complement(354..1442)
                     /locus_tag="PKN92_05100"
     CDS             complement(354..1442)
                     /locus_tag="PKN92_05100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ48201.1"
                     /translation="MKFVFYACETRKIAATRYRVDSFVRLLEAEGHHCTVCVPASVSM
                     REILYESGGRTGKFFYLLLVLLTRLLQLRHALFADAVFFRGPLFPYGPPLLEWLVLRI
                     NPQAVVDLDDALWERPAYVSSPFLRFVNFGWAAFMAKHCRCAAAGNGIIRDYMESHGC
                     PALIVPTCIDMELHGQKQYTAPGTKPVVLGWTGLSDNLGYLDTIVPVLERLAAKYPIE
                     LFVASGKAYAHEGNLPVRNEIWRTDREIAILQVADIGLMPLEDTPRARGKCAFKALQY
                     MGVGTPVVLSPVGMNREIVQHGVTGYLADTPDEWESALEKLISDASARESMGRAARTF
                     VAGHYAVDRWYPAFRTLLMQAAGKGKNL"
     gene            1701..2273
                     /gene="rfbC"
                     /locus_tag="PKN92_05105"
     CDS             1701..2273
                     /gene="rfbC"
                     /locus_tag="PKN92_05105"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNZ48202.1"
                     /translation="MPRRIKIYAAPLAGVVEIVAPVFADERGFFTEAYNRERWAEAGF
                     HEQFVQDNLSLSAKGVLRGMHYQLHPWGMGKLMRVLTGAAFDVLVDLRSGSPTYGKWM
                     GRHLEAADPRWLFAPPGFAHGFLALEDDTRMYYKCTSFYRPEAERALRYNDPELGIEW
                     PVSVTSLSEKDAAAPLFCDAETNFHYSDGA"
     gene            2350..3312
                     /locus_tag="PKN92_05110"
     CDS             2350..3312
                     /locus_tag="PKN92_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ48203.1"
                     /translation="MKLMIAGGAGYIGSVLSPMLQARGHDVDVVDLCWFGNQLPSDIP
                     VTRRDLFTVVPEDLEAYDQLIFLAGLSNDPMAEFDPAMNFIHNGALPAYLAFRAKQAG
                     LSRFIYASSCSVYGYTVDTLYDEESPAVCAYPYGISKLLGERGVLQEQSEHFSAIALR
                     QGTVNGYSPRMRFDLIVNTMFKTAVTEKKIRVNHPDIWRPLIDVRDTAEAFVRAVEAP
                     AEVSGVFNVAYDNFTIGDVAKAVRKEVESIYGIQVEIEMNRIADLRNYKVSSEKARAQ
                     LGFNPRFGIAEMVQSLAGHRDAYGDFTKREFYNIQVFKALAADA"
     gene            3337..4566
                     /locus_tag="PKN92_05115"
     CDS             3337..4566
                     /locus_tag="PKN92_05115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNZ48204.1"
                     /translation="MNNYFEHMLDRYIGKILCFLFSQVEKIRRVFQKKGDSAAPGKIL
                     FVKPVEQGAIILALPALKRAVDAVGRENVYFFCLRKNRVILDILDVLPRENVFTVDDR
                     SLFSFAAGLTHAVFQLRRLKIDTAIDLEIFARISIIIAWFSGARRRVGLHRFNSEGPY
                     RGNLVTHKVQYNPYIHTSQTFEVLVCAAFADPADTPLLKEPIPELWEALPQFQPAPED
                     REEAFRLLKTRLPGWTEETAAKDTLILFNPKCLDELPVRKWPDSSFVALGRKILQSYP
                     EATILITGLENEKAACEAMAREIGDGAISLAGVLSLRGLLALMTVCRVLVSTDSGPAH
                     FAALTDLAGVILFGPETPDLYSPLSSRMKLIYRHLTCSPCFSPMNYRLSPCRNNQCVL
                     QITPDQVFDAVREILEG"
     gene            complement(4631..5074)
                     /locus_tag="PKN92_05120"
     CDS             complement(4631..5074)
                     /locus_tag="PKN92_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48205.1"
                     /translation="MTKKTLILLPLFLLALSLSPAMLPTATADAKACCCGDNCKCPAD
                     ACKCADQCKCPAGACECPAGTCKVADACKCPAGACECPADACKSADNCAAAKSCKCPA
                     DACSSACQDKCTASSKGGCSEKCVASTGAKPCCPAGSACPVKDAA"
     gene            complement(5169..5621)
                     /locus_tag="PKN92_05125"
     CDS             complement(5169..5621)
                     /locus_tag="PKN92_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48206.1"
                     /translation="MVAKRTLVFLIACTSALLPYASMLWCACAEDCLHCTETVHNCHA
                     PEATPEHTGGSCCDSKMPSADSVPADTDYEGRPVAGTQCACGHGSTETNWLATIPATR
                     KSQINNLTAITSPPVADPSTHMVSGVSESPRPVHTASAPHVLHCVFLC"
     gene            complement(5774..6550)
                     /locus_tag="PKN92_05130"
     CDS             complement(5774..6550)
                     /locus_tag="PKN92_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNZ48207.1"
                     /translation="MGNPHSKTADFMRTYLTDLVTRYASRPSIWAWEVGNEYNLAADL
                     PNAADHLPMRAPSRGTPSERTMADAISSADILAAMQLFVDTVSPLDPHRLLTSGHSIL
                     RPSQYHQRIESSWKRDTTAQYCRELLRSHPEPFKLISVHLYPEAQTSGYFNLPFCSYE
                     HHLATLIATAAPAGKAVFVGEFGAASLEERSGAEGAQENFYQLLKAIETSGVPLAALW
                     VYDFPYQEKSCNVTSSNARSYQLEALKELNLRLQAPGQAP"
     gene            complement(6556..7008)
                     /locus_tag="PKN92_05135"
     CDS             complement(6556..7008)
                     /locus_tag="PKN92_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48208.1"
                     /translation="MYDSMKRTGKMCLIATVLLLAGGLPDPAAAETARDVVRLAVNED
                     GIVTLDGKPWRGVGVNFFSAFRRTLDNPEDTSYTASFQELAAFHIPFVRFMCGGFFPA
                     EWKQYLEDKEGYFARLDGVVEAAEKAGIGLIPSVFWFYAGVPDTVASL"
     gene            complement(7030..8835)
                     /locus_tag="PKN92_05140"
     CDS             complement(7030..8835)
                     /locus_tag="PKN92_05140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ48209.1"
                     /translation="MYRSVCFSPACRRLALLAAFLLLLPCSYADTDAVSEVMTQVTER
                     LREGRDEAALDALDHETVLAALTAEERHVLATEYWTFSVSAPAVISVVRHIEQKTVPF
                     WLTEEGFQKTDLIVRNELDSYEVWQKSFPAGKVGLGINGFERHRPHYFVAVGAKQPGD
                     QITVTDICPEPPALLSFAIGSFTYADWDELVLTEVPDALNGQILLPTIRGRAREAEII
                     GAFRKSPSVAGETPDLLTLTWSGEPKTTQAIQWRTAPGVENSGVYVRKQKDTEAQAWA
                     FTPATAVKLEDRNILNNVKVLWHTAEVVGLEPDTLYEYAPGTADLLPDKAAASFRTAP
                     DASNQFSFLWVSDNHSRPDSVPLIEQAHRRHPDAAFLAISGDLIGTGQHRDDWERLFT
                     LYSDFITTLPLVPAIGNHDTIDGLGSTLYRTLFRLPDNGPAGLHRGQSYSLEYGGLLQ
                     LISVDSTDTISGQSAWLEETLSHSKAPWKVVQFHFPPYAPDEDYPEIRSLWGALFDKY
                     HVDIVLSGHVHYYLRTFPMAGEKPVESPGNGTVYLVSVAVNGREQGEPAPDYAAVFKK
                     DGIATCQAFTVSAHTLTMNAYTADGSVYDSFALQK"
     gene            complement(8915..9166)
                     /locus_tag="PKN92_05145"
     CDS             complement(8915..9166)
                     /locus_tag="PKN92_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNZ48210.1"
                     /translation="MQKIIVYSADFCSDCQHLRAWMDKEGIAYEVRDIRKDPEHARTL
                     EEKTGKQGVPFLIIDGEWKRGYEPGKAFSEAFARSLFNR"
     gene            complement(9184..10503)
                     /locus_tag="PKN92_05150"
     CDS             complement(9184..10503)
                     /locus_tag="PKN92_05150"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNZ48211.1"
                     /translation="MKQKTIAKAASFNGIGLHTGNLTTVTFKPAPADSGIVFCRVDLE
                     EKPVVPALLNNVVDVSRGTTIGIGDVKIHTIEHAMACFAGLGIDNIIVEVDADEIPNG
                     DGSSLPVMDTLLSAGIVELDKKKRYITVDHPIYYRKDDVTLSILPSEEFRVSMTIDYD
                     HPAIGTQYASFTINEETFKNEIAPARTFCFLREVKMLQEAGLIQGGSLESAVLIGDDG
                     ILNETLHFQDEFVRHKILDLLGDTYLLGSPLKGHIIGVKCGHEKNVLFSKQIQKVFAS
                     TRPECELSAYRQQTRRQPAMLEVQKIGKVLPHRYPFLLVDKILSFDPLVSATGIKNVT
                     VNEPFLQGHSPEIPVMPAVLIIEAMAQMGAVLIFGDNGEPNGQVAFLMGVENVRFKNT
                     VLPGDQMLLKADMVHYRPNACKIRTTALVDDAVAAEAVMSFGLTQIE"
     gene            complement(10481..11533)
                     /gene="lpxD"
                     /locus_tag="PKN92_05155"
     CDS             complement(10481..11533)
                     /gene="lpxD"
                     /locus_tag="PKN92_05155"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNZ48212.1"
                     /translation="MQMRVKEIAALVQGTVLGDENIIIDQVNGIAEATAGQITFARDT
                     KHVHLLAESKAGAALVPCGTGSLPLTTIEVEQPDFAFFTVLKAFATVVEKPAPGIHPQ
                     SAVSADAEVHESAHIGPFCVVSAGSRIEEGVVLVANVFIGENCRIGANTRMYPHVVIR
                     EGTEIGKNCIIHAGACIGSDGFGFVPAGGVWHKIPQIGTVFIGDDVEIGSNTAIDRAT
                     FGVTRIGTGTKIDNLVQIGHNVVMGEHCAVAGMAGFAGSAVIGNQVRVGANAGMAGHI
                     TLGDGCTVGARAGVMRNVEPGAIVSGMPATDHRQQLRIMAAQGRTPEMLRRIAALEKE
                     LEALKEQKKNETKNNC"
     gene            complement(11694..12260)
                     /locus_tag="PKN92_05160"
     CDS             complement(11694..12260)
                     /locus_tag="PKN92_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNZ48213.1"
                     /translation="MTFGFIRRTGIISIAMATLLVASGAFVSVAFAQTGGEGSYKIGV
                     VDMSTLVNESNKRKAKYDELQQEVDRLQTEIDAMSKRIEKAKEDFEAQRQTMTDDQRF
                     EKRSQIESDFAQYRSELDRRQRLIDTQEERVLKEVVDDIDRVVAKVAEDEGYHLILNA
                     SKGPRATVLFHSATIDLTSRVLQIINKK"
     gene            12533..14194
                     /locus_tag="PKN92_05165"
     CDS             12533..14194
                     /locus_tag="PKN92_05165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNZ48214.1"
                     /translation="MITTACHYCGLTILVPESMAGREGRCLNCGEAIRVPRTAQQPHQ
                     SHTEFAAGDVVNERYRIQKFIGKGGMGVVYLADDTLVKESVALKFLRPDLLQTARAKR
                     LFLQEAQIARRLRHEHIIAVHDISATAAGILFFTMEFAEGKAIRTLLMEQRQQRKYLP
                     VRLVHTIALQVLSALSYAHRLVVHRDIKPENIQLLPNERIKVLDFGLAIAVEEEETLL
                     TASRSKSKIMGTLAYAAPEQLLHTPVDARTDLYALGLVLRELLSLKTPIELPPEEPLV
                     RDDVPPDYLSVINKAVRKDKNDRWQSAAEFSNALQASFDQSFFIVTSEHVPAASSAVP
                     TREGMVFLEGGRFLMGSNLVPECAPEREVEVAPFWMDIYPVTVKEYARFIEETGAEKP
                     AFWSNPQFNGDDQPVVGVTWEEARAFARWAGKELPTEAQWEFAARGTENRKYPWGSLR
                     PEPSRCNFNRYLGMPSIVSMHEEGRTPQGIFDLAGNVYEWTRDPWRPYCARPDSAEAA
                     EVRPRKAIRGGCYESAAAEMTTSWRKGEFHNVRLATLGFRCVIPL"
     gene            14305..14898
                     /locus_tag="PKN92_05170"
     CDS             14305..14898
                     /locus_tag="PKN92_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48215.1"
                     /translation="MRAKSEDGRLHALLNFRESGQVRKKNFVTVFSLGVCLLCIPVLT
                     SFLDRKAPDPVHAFKVHVNEGRVVLTWINPAGNDWAGVLVCRKAGEPPKEPLDGFVLV
                     SGAVDRAEDVEVTGGETYYYAVWTYDVLGNFSPAVRGFASLPSGVRQRMVEGERTVSP
                     LTDTVLMQKSGRLSAISAGTVQCAVLSADSVKSAAGV"
     gene            15125..17584
                     /locus_tag="PKN92_05175"
     CDS             15125..17584
                     /locus_tag="PKN92_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNZ48216.1"
                     /translation="MWSRFTERAKHVIATAREEAVRLGCEYVRTEHILLGLCKEREGI
                     AARALKNLDIDIDMMEEEIVRLTFSSANATPSEEITFTPRAKRVLELATTEAHRVSHN
                     YVGTEHLLLGLLQEGEGLAAKIFEDLKVDTKRLQEEIFRLMSTQVRPEMQQAQAPAKS
                     QTAALDAFSRDLTQMARDNKLDPVIGREDEIERVIQVLSRRTKNNPVLIGEAGVGKTA
                     IVEGLAQAIVSGDVPELLLNRRVLALDLAGMIAGTKYRGQFEERMKAVMREIRKADNI
                     ILFIDELHTLVGAGAAEGAVDAANMLKPALSRGEIQCVGATTMDEHRKYIEKDSALAR
                     RFQTILVNAPTVTETIEIIKGLRDKYEAHHRVRFTDEAIIAAAQLSNQYITDRSLPDK
                     AVDVIDEAGSRARLQLTTRPASLKQISVDIEQATQEKEAAISNQEYEKAAALRDRERT
                     LIARLESEKRLWEQKRDSTDMEVTAEDIAFVVSKWTGVPLTKLEEKESARLLRMRDEL
                     HKTVVGQNEAIDAITRAIQRSRAGLRGRTRPVGSFLMLGPTGVGKTLLGKALAEFLFG
                     NQEALITVDMSEFMEKFSVSRLIGAPPGYVGYDQGGQLTEQVRRRPYSVVLFDEIEKA
                     HPDIFNALLQVLEEGHLTDSAGRKVDFRNTVIMMTSNIGARRIGKNTSLGFYRDTADE
                     NYERMKERVMEEVQKIFNPEFLNRIDEVLVFHRLGHEELLEIVDLQVNEIVARVAEQS
                     ICLEVTPEAREFLLRMGSNEEYGARPLRRAIQQYVEDPLAEMLLKGNLSPDTAIVMRP
                     SDEGNALVPEAMAETTGGMLS"
     gene            17581..19899
                     /gene="bamA"
                     /locus_tag="PKN92_05180"
     CDS             17581..19899
                     /gene="bamA"
                     /locus_tag="PKN92_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNZ48217.1"
                     /translation="MNTKSLFTPLAVLLLISGMVCVGIAGAQEGEARIIRDISFDGLL
                     RISDAAARAQLETQVGAAYDAGAVARDIKRLMDLGFFDAVDAKVEEAGGEIILTYAVV
                     EKRLIDQILIVGNRKIKSRVIRAALTMREGGSFEPEMFENERQAVLDLYKGKGFANTT
                     VDVTAENIGPSRVRLVYTIGEGKKARIKSITFVGNDHMKTKRLRKVMKTKQARWFFSS
                     RYDEDKFAADLTNITDAYGELGFLEAKVASTDMEYSNNGKRIDITMYLEEGPQYSMNS
                     LDIARNIVYDDEDLLDVVKVHPGDVHNKVQVSKDVAAIQQGYQDSGYIDAVVTPQITL
                     DRENKTTNVVYHVEEGNLKYLREIKITGNDITKDEVIRRQMLLIPGERYDGAAVTESE
                     NRIQNTRFFDKVRITFDETDDELFTDLRVNVEEGKTGVFNFGAGYSTEDRLGVYTEMR
                     LSNFDLFNWPRFSGAGQVLRLRTQIGERRNEYSISFTEPEFLGYPVSFGVDLFDESYE
                     VRGAARYKEEIRGGQLRLGKSLSPYVFAQSIWRFQETDLSQFSRWVHPEIRKQEGRST
                     TIANRWQIERNTLDSNFDPTRGSVHVLAAEIAGFGGDHNFLRFEQDSTWYRALGEKEK
                     IVLSLRARHGIMTTYGSSDYIPLQERFYAGGTTTVRGYRNRDIGPKVREYRWWGDHFA
                     VGGDTRFVYNAEVKYRVTDIFRLYGFADAGAVWADAGDIDFGDFNYSVGAGVGFNVPM
                     FGPIRVDYGYPLNPNKNQSSSGRLHMSTGFRF"
     gene            complement(19990..21270)
                     /locus_tag="PKN92_05185"
     CDS             complement(19990..21270)
                     /locus_tag="PKN92_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNZ48218.1"
                     /translation="MKVFIQTAITLFLLQSITCGAAQAQKIIPGHSLIIALPGKTLLA
                     QDRDLLKEIQPGGVLLLGNNISNRQQTLNLVAAIKRTVSGKAFSGALPLIYVDQEGGR
                     VNRLRLDNAPSASSLGAEGSEGKIRDTAIAYARAARERGIEVVIAPVLDLKVPGSRAI
                     GDRAFSDDPEIAWLFGKRFMDGILQEGLLPVVKHFPGHGAAIEDSHVQLAHLNLTGQA
                     LEDTLLPFKNAVDATVPAVLVGHIACPALDPEFPDTPATLSRAMVTGILREHWGYDGF
                     IMTDDMNMKAVKGALEDLVVRSLRAGCDAVIICEHNPALLRKIVRTIALEAENDPQFL
                     DMLRQSRKRLDRFSSRIFPVDIQPEAATAVATQTPRQPGSGQETIIQHKVAAGDTLSK
                     LAVRYGTTTETIRQYNNKNSDVIRIGETLHIPGN"
     gene            complement(21418..22176)
                     /locus_tag="PKN92_05190"
     CDS             complement(21418..22176)
                     /locus_tag="PKN92_05190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNZ48219.1"
                     /translation="MHSGWERNQIYNEDCLTGLKRLPDESVDLVITDPPFAIDFTPLR
                     DNYKRHPHLVLGGYQEVKTVDYPDFTRNWMTQIRRVLKESGAAFIFSGWNNLKDILVT
                     ADALNLITVNHLIWKYQFGVVCKYRFVTSHYHCLYLCKDNKQRKFFHNARFSSDDRTA
                     TGRSKRYADLEDVWTIQREYWHGDMKTPTKLPGELIRKILAYTSEPGDLVLDPFLGSR
                     QVAVISRESGRDYIGFEIVPAYFEFAKSRLAEMP"
     gene            complement(22193..24211)
                     /locus_tag="PKN92_05195"
     CDS             complement(22193..24211)
                     /locus_tag="PKN92_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNZ48220.1"
                     /translation="MPAISKKTTTLSKYHVLYVLFLYISVSHFAFGQLTPEDIADLQQ
                     QGKEKGWTFSVGLTEASQRPLEQLGNVRFTEEALREEEEHQMDPALLNKAGLPSRFDW
                     RDHVSRPLPPVKDQGSCGSCWAFATTEVLEWAIKIHDDTVVDLSEQWLISCNSEILAH
                     TCSTGGFMQLGYFKDKKDDCGSSGAVLEKDCPYTGWDSPCKCPYPHKYWIKSYSQLGT
                     FLNIAPVEDIKAALYQYGPLGTYLKISKSLIAYTGGVYNDKKDEKAWNVPDHAVIIIG
                     WDDSKGAWLVQNSWGTGWGMGGYGYMEYGVDLIGSSASWVIYGDDLSVSPRDLSFSAE
                     AGSSSSIASAQNTLLLENKGTAPFSWSATAPTWLKLSANQGTIAPGTSVTLSLTLALD
                     TVNLQPGNYSGTIFVTNQSSASVQSVPAALTLTPPVIYNFPLDSNPGWSADTGWAFGR
                     PAGLGSYNRDPSRAYTGQYVYGYNLKGDYESDLPRRSLTTPSLDFSRASGVTLRFMRW
                     LGVEEGQYDEAAVEVSNNGYTWITVWKNPARDEVLDTEWVECEYNLSAVADGQPNVRV
                     RWVMGPTDYSQTYPGWNIDDIQFRGRVETEAEVEGEPEPRHPADTDNDGHFSQNEALS
                     YISQWQKGTREMKHAVQILFIWKKGGYYRYDETKEEPYCWVPIENPGN"
BASE COUNT         5690 a         6240 c         6827 g         5739 t
ORIGIN      
        1 aggcaaggct gcgcagcagt tcaaggcagt gtttcgcttt ttcaagatga attccgtccc
       61 cggtaatttg aaagagccgc aggtgcccca gagcaaaaag cgccggtgtc ttcggattaa
      121 ggctgcggtc aatacggagc atgcgtcgca gtaccgtcgg gaaggcgtcg cccagaacag
      181 tggtaacttt tcgaagccag ggcctctgct gtgctttttt gaaaagagga tgcgctttga
      241 tgtcaaaagg gtcaaagccg ggcgtgtccg gcgcctgcat cagggcggac agttgaatga
      301 tccattcctg agcaatatga atgtcacggt tattcaccag ctgcaaaggc attttacaaa
      361 ttctttcctt taccggctgc ctgcatcagc agggtgcgga atgccggata ccaacgatcg
      421 acggcgtaat gtccggcgac aaaagtccgg gcagcccgcc ccatgctttc gcgtgccgaa
      481 gcatcgctga ttaatttttc cagagcagac tcccattcgt cgggtgtgtc ggcaagatac
      541 cctgttacgc cgtgctgcac gatttcacga ttcataccga caggcgacag aacaacaggt
      601 gtcccgactc ccatatattg cagtgccttg aaggcgcatt tgccccgcgc ccgcggcgta
      661 tcttccagag gcatgagacc gatatccgct acctgcagga tagcaatttc ccgatctgtc
      721 cgccagattt catttcgaac cggcaggttc ccctcatggg cataggcttt tcccgaagcg
      781 acaaaaagtt ctatcggata tttggcagcg agccgttcca gtacaggaac aattgtgtcc
      841 agatagccca gattgtcgct cagtccggtc cagcccagca ccacaggttt ggtgccgggg
      901 gcagtgtatt gcttttgccc atgcagttcc atgtcaatgc aggtgggcac aatcaatgca
      961 gggcagccat gggactccat atagtcccgg atgatcccgt tgcctgccgc tgcgcagcgg
     1021 cagtgctttg ccataaaggc agcccagccg aaatttacga accgcagaaa gggactggaa
     1081 acataggcgg gccgctccca gagcgcatca tccagatcga caactgcctg cggattgatt
     1141 cggaggacga gccattccag cagaggcgga ccgtagggaa aaagaggccc acgaaagaag
     1201 accgcatcgg cgaagagcgc atggcgcaac tgcagcaggc gggtgagcag caccagcagc
     1261 agatagaaaa actttccggt acgcccgcca gattcataaa gtatttcgcg catggaaaca
     1321 gaagcaggaa cacacacagt gcaatgatgc ccttccgctt ccagaaggcg gacgaaggag
     1381 tctacacggt atcgtgtagc agcgatcttc cgagtttcac aggcataaaa cacaaacttc
     1441 ataaagggta tgattccgga aaaggggttg aagcggcggg cgcagcatcg gttgcccggc
     1501 ctctgcatat tatgcctgcc gtcgaagcgg agtgcaaaca cagcgtcgca ggattatcgg
     1561 aaaattaaag agcctttttg ctgccgccgc cccccgtcgc aacggcttct ttgctgcaat
     1621 gtatctctat tcattataat ggctggagat ttacttatga atattcgtgg tcagaatcac
     1681 aagcaaggaa agatgtttta gtgccgcgac gcatcaaaat ttacgctgcc ccgctggcag
     1741 gtgtggtgga gattgtagcg cctgtttttg ccgatgagcg cggttttttt acagaggcct
     1801 ataaccgcga acgctgggcg gaagcgggct ttcatgagca gtttgttcag gacaatctga
     1861 gtctttcggc taaaggggtg cttcgcggaa tgcactatca gctgcacccg tggggcatgg
     1921 ggaaattaat gcgggtactg accggtgccg cctttgatgt actggttgat ctgcgctccg
     1981 gctccccgac ctatgggaaa tggatgggcc gtcatctgga agctgcagat ccgaggtggc
     2041 tttttgcgcc gcccggtttt gcccacggat ttcttgctct tgaagatgat acacgcatgt
     2101 attacaaatg cacttctttt taccgtccgg aggctgagcg tgcacttcgg tacaatgacc
     2161 cggaactcgg gattgaatgg cccgtatctg tgacaagcct ttctgaaaaa gatgcagcgg
     2221 cacctttatt ctgtgatgcg gagaccaact ttcattatag tgacggtgcc tgaccggccg
     2281 ctttcatgct ttttgctgtg ttttccgttt tgaaattttc tgaagtattt gacagggaga
     2341 acgaaataaa tgaaactgat gattgcaggc ggagccggat acataggctc tgttttgtct
     2401 cccatgcttc aggcacgggg gcatgacgtg gacgtggtgg acctatgctg gttcggcaac
     2461 cagctgccat cggatatacc tgtgacccgc cgggatttgt ttaccgtggt gcccgaagat
     2521 ctcgaagcat atgaccagct gatatttctt gccggattga gcaatgatcc catggcggaa
     2581 tttgatcccg ccatgaactt tatccataat ggcgccctgc ccgcttatct tgcattcaga
     2641 gcaaaacagg cgggactgtc ccgttttatt tatgcctcct cctgttccgt gtacgggtat
     2701 acagtcgaca cgctttacga tgaagaatct ccggctgttt gtgcctatcc ctatggcatt
     2761 tccaaactcc tgggagagcg cggcgtgctt caggagcaga gtgaacattt ttcagccatt
     2821 gccctgcggc aggggaccgt aaacggctac agcccgagaa tgcgttttga tctgatcgtg
     2881 aacaccatgt ttaaaacggc ggtgaccgag aaaaaaataa gagtgaatca tccggatatc
     2941 tggcgtcctc tgattgatgt gcgggacacg gcagaagcct ttgtccgggc tgtcgaggcg
     3001 cctgctgaag tcagcggtgt ctttaatgtg gcatacgata attttacgat aggtgatgtg
     3061 gcaaaagccg ttcgtaagga agtggaatca atatacggca ttcaggtgga aatagagatg
     3121 aaccgcatag cggatctgcg taattataaa gtgtccagcg aaaaagcacg tgcgcagctg
     3181 ggcttcaatc cgcgcttcgg gatcgcggag atggtgcagt ctcttgccgg gcatcgggat
     3241 gcttatggcg attttacaaa acgggaattt tataacatac aggtgttcaa ggcgcttgct
     3301 gcagacgctt agacggaaat acttttgcaa taacccatga ataactattt cgaacatatg
     3361 ctggatcgtt atatcggcaa aatcctctgc tttctttttt cgcaagtgga gaaaatacgc
     3421 cgtgtttttc aaaaaaaagg agacagcgcc gctccgggca aaattctttt tgtaaagccc
     3481 gtggaacagg gcgccattat cctcgccctg cctgccctga aacgcgctgt ggatgcagtg
     3541 ggccgggaaa atgtgtattt tttctgtctg cgtaaaaata gagtgatcct ggatattctg
     3601 gacgtgctgc cccgggaaaa tgttttcact gttgatgacc gttctctgtt ttcctttgcg
     3661 gctggactga ctcacgctgt cttccagctg cgcaggctga aaatcgacac agcgattgac
     3721 ctggagattt ttgcccggat ctccattatc atagcctggt tcagcggggc gcggcgaagg
     3781 gtgggactgc atcgtttcaa cagtgaaggg ccttaccgcg ggaatctggt gacgcataag
     3841 gttcaatata acccgtatat ccacacttct cagacctttg aagtgctcgt ctgtgctgct
     3901 tttgccgatc cggcggacac gcctcttttg aaagagccga taccggaact gtgggaggca
     3961 cttccacaat ttcagcccgc cccggaggac agggaagagg cgttccggct gcttaagacc
     4021 cgtctgcccg gatggacgga agagacggct gccaaggata cgctcatcct ctttaatccg
     4081 aaatgcctgg acgagctgcc tgttcgcaaa tggcccgaca gttcctttgt cgcattgggc
     4141 agaaaaattt tgcagtccta tccggaagca acgattctta ttacaggact ggaaaacgaa
     4201 aaagcagcct gtgaagccat ggcacgggag attggcgacg gcgccatcag ccttgccggt
     4261 gtattaagcc ttcgcggtct ccttgcgctc atgactgtgt gccgcgtact ggtttccaca
     4321 gacagcgggc ccgctcattt tgcagcgttg accgatcttg ccggagtgat ccttttcggc
     4381 ccggaaacac ctgatttgta cagcccgctc agttcgcgca tgaaattaat ttacaggcac
     4441 ctgacgtgca gcccctgctt ttcccccatg aactacaggc tttctccctg tcggaacaac
     4501 cagtgtgttt tgcagattac cccggatcag gtctttgatg ctgtccgcga aatactggag
     4561 ggataaaaag gagggcgcag ccccgtacgg aactgcgccc cggagagtta caggggagga
     4621 gagagcttac ttatgcggca tctttaaccg ggcaggcact tccggcaggg cagcatggct
     4681 ttgcgcccgt ggaagcgacg cacttctcgg aacatccgcc cttcgaagag gctgtgcact
     4741 tgtcctggca ggctgatgaa caggcatcag cagggcattt gcagctcttg gcagccgcac
     4801 agttgtcagc gcttttgcac gcatcagcgg ggcattcaca tgcaccggca gggcatttgc
     4861 aggcatcagc cactttgcag gtacctgcag ggcattcaca tgcaccggca ggacatttgc
     4921 actgatcggc gcatttgcag gcatcggcgg gacatttgca gttgtcgccg cagcaacagg
     4981 ctttggcgtc ggcggttgcg gtaggcagca ttgccgggct cagggaaagg gcaaggagga
     5041 aaaggggaag cagtatcagt gttttcttgg tcatgagtat tttccttttg ttgaactcaa
     5101 gagtaaacga gaattttggt gtttgaagtg aaagaaataa cgatcgggaa aagttcagac
     5161 acacaaaatc aacacaggaa aacacaatgc aggacatggg gcgctgaggc tgtatgaaca
     5221 gggcgggggc tttcagaaac acccgacacc atatgcgtag acgggtcagc cacgggcggg
     5281 ctggtaatgg ctgtcagatt gttgatctgg cttttgcgtg tcgcaggaat ggttgcgagc
     5341 cagtttgttt cggtgctgcc atgcccgcat gcgcattggg tgccggcaac gggcctgccc
     5401 tcataatcgg tgtctgccgg cacagaatca gcagacggca ttttgctgtc acagcaggat
     5461 ccgccggtgt gctcgggcgt tgcttccgga gcgtggcagt tgtgaacagt ttctgtgcaa
     5521 tggaggcagt cttccgcaca ggcgcaccac agcatggaag catagggcag tagcgcactg
     5581 gtacaggcga tcaaaaagac caatgtacgc ttcgcaacca tcttttacat ccttctttgt
     5641 tctactgctc tgtagggagt ataccacagg tttcggcaaa agtcaagtaa gccgaccttt
     5701 ttactctgta ttagaaaaac cggacaggag gcacagcaga aaaagaataa atgaccccca
     5761 ctattcagcg caatcagggg gcctgcccgg gggcctgcag gcgtagattc agctctttta
     5821 atgcttcaag ctgataagaa cgggcattgg aagaagtgac attgcaggat ttttcctgat
     5881 agggaaaatc gtaaacccag agggcggcaa ggggcacccc gctggtttca atggctttta
     5941 agagctgata gaaattttct tgtgcccctt cggcgccgga tctttcttcg agactggccg
     6001 cgccgaactc tcccacaaac accgcttttc ctgcaggggc agctgtggcg atgagtgtgg
     6061 caagatgatg ctcataggag cagaaaggaa gattgaaata accgcttgtc tgcgcttcgg
     6121 gataaagatg gacggagata agcttaaaag gctcggggtg actgcgcagc agctctctgc
     6181 agtactgcgc cgttgtgtcg cgtttccagg agctttcgat gcgctggtgg tactgggaag
     6241 gccggaggat ggagtgcccc gaagtcagga ggcggtgagg atccagcggg ctgacagtgt
     6301 ctacgaaaag ctgcattgcc gccaggatat cagcgcttga gattgcatct gccatagtcc
     6361 gctcagaggg ggtgcctctt gatggtgccc gcatgggcag gtgatccgcc gcattgggca
     6421 aatcagccgc gagattgtat tcgttgccga cttcccaggc ccaaatggac gggcgggaag
     6481 cataacgcgt cacgagatcg gtgaggtagg tgcgcatgaa atcagccgtt ttgctgtgcg
     6541 gatttccaat ctgatttaca ggctcgccac tgtatccggc acgccggcat aaaaccagaa
     6601 cacgctggga atgagcccga taccggcttt ttcagccgct tccacaacac catcaagccg
     6661 tgcaaagtac ccttccttgt cctccagata ctgcttccat tcggcaggga aaaaaccgcc
     6721 acacataaag cggacaaaag gaatatgaaa agcggcgagt tcctgaaagg aggcggtata
     6781 ggaagtgtct tcggggttgt ccagagtgcg ccggaatgcg gagaaaaaat ttacaccgac
     6841 acctcgccag ggctttccgt caagggttac aataccgtcc tcattaacgg caagccgaac
     6901 cacgtcccgg gcagtttcag ctgctgccgg atccggcagg ccccccgcca gcagcaggac
     6961 agtcgctata agacacatct tacctgtgcg cttcatcgaa tcatacataa gagctgcttt
     7021 cagcgggctt tatttttgca gagcaaaaga gtcatacacg gaaccgtcgg cagtatacgc
     7081 attcatggtc agtgtatgcg ctgatacagt gaaagcctgg caggtggcga tgccgtcttt
     7141 tttgaaaaca gcggcgtagt ccggcgccgg ttctccctgt tctcttccgt tcacagcgac
     7201 tgaaaccaga taaaccgtac cgttgccggg gctttctaca ggcttttctc cagccatggg
     7261 aaaagttctc aaatagtaat gcacatgccc tgagagcaca atgtccacat ggtacttatc
     7321 gaaaagagca ccccagaggc tgcgaatttc agggtagtcc tcgtcgggtg cgtagggagg
     7381 gaaatggaac tgaaccactt tccagggggc cttcgagtga ctgagtgttt cttctagcca
     7441 cgcagactga cccgaaatcg tgtcggtgga atcgacagaa atcagctgca gcagccctcc
     7501 gtattcgagg ctgtagcttt gaccccggtg cagcccggca gggccattgt ccggcaggcg
     7561 aaacagagtg cggtacaagg tggatccgag accgtcgatc gtgtcatggt tgccgatagc
     7621 gggtaccagt ggcagtgtgg taataaaatc gctatacagc gtgaaaagcc gttcccaatc
     7681 gtcgcgatgc tgtcccgtac cgatcagatc tccggaaata gcgagaaagg cggcatcggg
     7741 atggcggcgg tgcgcctgct cgatcagcgg gacgctgtca ggccttgagt ggttgtcgct
     7801 cacccaaaga aaagaaaact gattcgaagc gtccggtgct gtcctgaaag aagcagctgc
     7861 tttgtccgga agcaggtctg ccgttccagg tgcgtattca taaagagtgt ccggttccag
     7921 cccgacaact tcggcggtat gccagagtac tttgacatta ttcagaatat tgcggtcttc
     7981 cagttttaca gcagtggcgg gtgtaaacgc ccacgcttgt gcttctgtat ccttctgctt
     8041 gcgcacataa acacccgaat tttccacacc gggggcagtg cgccattgga ttgcctgagt
     8101 ggttttcggc tcgccgctcc aggtaagtgt cagcagatcg ggcgtttctc cggcgacgga
     8161 cggactcttg cggaaagcgc cgataatttc cgcttcccgg gcacgaccgc ggatagtggg
     8221 caggagaatt tgaccgttca acgcatccgg cacctctgtg agcaccagtt cgtcccaatc
     8281 ggcataggtg aaagagccga tggcgaaaga gagcagggcg ggcggttcag ggcagatgtc
     8341 cgtgactgta atctgatcgc ccggctgctt tgcgcccaca gcgacaaaat aatggggtct
     8401 gtgccgttca aagccgttaa tgcccagacc tactttcccg gctggaaaag atttctgcca
     8461 gacttcgtaa ctgtccaatt cattacgaac gatcagatca gtcttctgaa agccttcttc
     8521 tgtcagccag aaaggtacgg ttttctgttc aatatgccgc actacagaaa tgacagccgg
     8581 cgcagaaacg gagaaagtcc aatattcggt ggcaagtaca tgccgttcct cggcagtcag
     8641 tgctgccagc acggtttcgt gatccagtgc atccagagcg gcttcgtctc tgccttcccg
     8701 gagtctttcc gttacctggg tcatgacttc ggagacagcg tcagtgtcag catagctgca
     8761 gggcaggagg agaagaaagg cggcaagaag ggcaagacga cggcaggcag gggagaaaca
     8821 gacagaacgg tacatgaggc agctccttca catgtggtga tccgacgccg ggcggaagtg
     8881 aaagggcgcc ggatggaaaa gcaggccggt atattcagcg gttgaaaaga ctgcgggcaa
     8941 aggcttcgct gaaggcttta cccggctcgt aaccgcgttt ccattccccg tcaataataa
     9001 gaaagggaac accctgtttt ccggtttttt cttccagtgt gcgggcgtgt tccggatcct
     9061 tccgtatgtc acggacttcg taggcgatgc cttccttatc catccacgcg cgtaaatgct
     9121 gacagtcact gcaaaagtca gcactgtaga caataatttt ctgcataagt gcgttccagt
     9181 tttttattcg atctgggtca gcccgaaaga cataaccgct tccgccgcaa cagcatcatc
     9241 taccagggcg gttgttcgta ttttgcaggc attgggccga taatgcacca tatccgcctt
     9301 aagcagcatt tgatcgcccg gcagcacggt gtttttaaaa cggacatttt caacgcccat
     9361 gagaaaagcg acttgcccgt tgggttcgcc gttatctccg aaaatcagca cagcccccat
     9421 ctgcgccatg gcctcaatga tcagtacggc gggcatgacc gggatttcag ggctgtgccc
     9481 ctgcagaaag ggttcattaa ccgtcacatt tttaatgcct gtggcactga ccagcgggtc
     9541 aaaactcaga atcttgtcga ccagaagaaa aggatagcga tgcggcagta ctttgccgat
     9601 tttctggact tcgagcattg ccggctggcg ccgggtctgc tgtctgtatg cactgagttc
     9661 acattcaggc cgtgtgctgg cgaacacttt ctgtatctgc ttggaaaaga ggacgttttt
     9721 ctcgtgcccg cattttactc cgatgatatg ccctttgagc ggggagccca gcaggtaggt
     9781 gtcaccgagc aaatcaagga ttttatggcg gacaaattca tcctgaaaat gaagggtttc
     9841 gttcaggatt ccgtcgtcac cgataagcac cgcactttca agactgccgc cctgaatcag
     9901 ccccgcctcc tgcagcatct tcacttcccg gagaaaacag aaagtccgtg caggcgcaat
     9961 ttcatttttg aaggtctcct cattgatggt aaaggaggca tattgcgtgc cgatggcggg
    10021 gtggtcgtaa tcaatggtca tactgaccct gaactcctcc gagggcagga tgctcagcgt
    10081 cacatcgtct ttgcggtaat agatcgggtg atcgacggta atgtagcgct tttttttgtc
    10141 gagctccaca atgcctgcag agagaagggt gtccataact ggcagggaac tgccatcgcc
    10201 attggggatt tcatcggcat ccacttcaac gatgatgtta tcgatcccga gccccgcaaa
    10261 gcatgccatg gcatgttcaa tggtatgaat cttaacatcg ccgatgccta ttgtggttcc
    10321 ccgcgatacg tccacaacat tattgagcag cgccggaaca accggctttt cttccagatc
    10381 cacccggcag aaaacaattc cgctgtcggc gggggcgggt ttgaaagtaa ccgtggtcag
    10441 attgccggtg tgcaacccta taccgttgaa agaggctgcc ttagcaattg ttttttgttt
    10501 cattcttttt ttgttctttc aatgcctcga gctctttttc gagggcggca atacggcgaa
    10561 gcatttccgg ggttcttccc tgggctgcca tgatgcgcag ctgctgccgg tggtcggtag
    10621 caggcatccc ggaaacgata gcgccgggct ccacattgcg catgacaccg gcacgggctc
    10681 caacagtgca gccgtcaccc agggtgatat gccctgccat accggcattg gcaccaacac
    10741 gcacctgatt gccgatgaca gcactgccgg caaaacccgc cattcctgcc acggcacaat
    10801 gctcacccat aacgacattg tgccctattt gcacaagatt gtcaatcttg gttcccgtgc
    10861 cgatccgggt gacaccgaag gtggcgcggt caatagcggt attacttcct atttcaacat
    10921 catctccgat aaaaacagtc ccgatctgcg ggattttatg ccagacaccg cccgccggga
    10981 cgaagccgaa gccatcgctg ccgatgcagg caccggcgtg gatgatgcag tttttcccga
    11041 tttccgtacc ttcacggata accacgtgag ggtacatgcg tgtgtttgcg cctatgcgac
    11101 agttttcacc gataaagaca tttgccacca gaacaacgcc ttcctctatg cggcttccgg
    11161 cggaaacaac gcaaaaagga cctatgtgcg cgctttcgtg cacctcggca tcggcagaca
    11221 ccgcactttg ggggtgtatg cccggagccg gtttttcgac gacggtcgca aaggctttga
    11281 ggactgtaaa aaaagcaaaa tcaggctgct ccacttcgat ggtggtgaga ggcaggctgc
    11341 ctgttccgca gggcaccagg gctgcacctg cttttgattc agcaagaagg tgcacatgtt
    11401 tggtgtcgcg cgcaaaggtg atttgtccgg cagtggcttc tgctatgcca ttgacctggt
    11461 cgataataat attctcatcg cccagaacgg tgccctgtac cagtgctgct atctctttta
    11521 cacgcatctg catagcgcaa gataaactcc cgtggagggg gaacaggatt cacagacttt
    11581 ttctgtgaga cccgtgtgcc ctgataaaac agcaaagcgc ttcgactatc gaagcgccgc
    11641 cgattttcac tatccgtatt gcctgagatc ccgaatttca gttttgtccg ggattatttt
    11701 ttattgatga tttgcagcac cctggaagta aggtcaatag tggcgctgtg aaacagaact
    11761 gttgcccggg gacctttcga agcattcaga atcaggtgat agccttcgtc ttccgccact
    11821 tttgcgacga cgcggtcgat atcgtcaacc acttctttca gcacccgctc ttcctgagtg
    11881 tcaataaggc gttgacggcg atccagttcg gagcggtact gggcaaaatc agattcgatc
    11941 tgggaacgct tttcgaagcg ctgatcgtcc gtcatcgtct gacgctgggc ttcaaagtct
    12001 tcctttgcct tttcgatccg tttggacatg gcgtctattt ctgtctgcag gcgatccact
    12061 tcctgctgca gctcatcgta tttagctttg cgtttattgg attcgttgac cagcgtggac
    12121 atgtcaacca ctccgatttt ataactgcct tcgccgcctg tctgggcgaa agcaacagat
    12181 acaaaagccc ccgatgcaac cagcagcgtt gccattgcaa tagagatgat gcctgtccgc
    12241 cggatgaaac caaatgtcat actcactatt ctttcccttc agtaatggca ctttcgcctt
    12301 cagattgttt tgtacgggtc aaaaggatgg cacacgcttg taagcacatg taatagcagt
    12361 atatcatgat cggtcaaaag tgttcaatgc ctgtgccatt aattgcagcc tgctctttat
    12421 catatccgaa aaagatggcg caaagcactg ccgttggaaa aaaggggagg caacaggtca
    12481 taatgaatgt actcccgtcc cgatgcagca agtattcagc aggtgcccgt tcatgatcac
    12541 caccgcatgc cattactgtg gattaacaat tctcgttcct gagtccatgg caggacgtga
    12601 gggaagatgc ctgaactgcg gcgaagccat ccgagttccc cgtactgccc agcagccaca
    12661 tcagtctcat actgagtttg ctgcgggcga tgtagtgaat gagcggtaca ggatccagaa
    12721 atttatcggc aaaggcggca tgggcgtggt gtatctggca gatgacacgc tggtgaaaga
    12781 atctgtagcc ctgaagtttc tgcgccctga tctgcttcag accgcccggg cgaagcgcct
    12841 ttttcttcag gaagcgcaaa ttgcgaggcg tttgcgtcat gagcacatta ttgcggttca
    12901 tgacatcagc gccactgcgg caggtattct ttttttcaca atggaatttg cggaagggaa
    12961 ggcgatccgt acactgctga tggagcagcg ccagcagcgc aagtatctgc ctgtccggct
    13021 ggtgcatacc atagcccttc aggtgctgag tgccctatcc tatgcccatc gcctcgtggt
    13081 tcatcgcgat atcaaaccgg aaaatataca gcttctgcct aatgagcgca taaaagtgct
    13141 ggacttcggg cttgcgatag cggtagaaga ggaagaaaca cttttgacag ccagccggtc
    13201 aaaaagcaaa atcatgggca ccctcgccta tgctgctccg gagcagttgc tacacacgcc
    13261 ggtggatgcc cgtacggatt tgtatgcgct ggggcttgtg ctgcgtgagc tcctctccct
    13321 caaaacaccg atagaactgc cgccggaaga gccgctggtg agggatgatg tgcctcctga
    13381 ctacctgtcg gtcatcaaca aagctgtccg gaaggataaa aatgaccggt ggcagtctgc
    13441 tgccgagttc agcaatgcgc tgcaggcatc cttcgatcag tcctttttta tcgtgacttc
    13501 tgaacatgtt ccggcggcga gcagtgcagt accgacccgt gaaggcatgg tctttctgga
    13561 agggggccgt tttcttatgg gcagcaatct ggtgccggaa tgcgctcctg agcgggaagt
    13621 ggaggtcgct cctttctgga tggatattta tcctgttacc gtaaaggaat atgcccgttt
    13681 tatagaagaa acaggcgcgg aaaagccggc tttctggagt aatccgcagt ttaacggtga
    13741 tgatcagcct gtcgtgggcg ttacctggga agaagcgaga gcattcgcac gctgggcggg
    13801 aaaagaactt cccacggagg cgcaatggga gtttgccgca cggggaacgg aaaacagaaa
    13861 atacccctgg ggcagccttc gccccgagcc ctcccgatgc aacttcaaca gatatctggg
    13921 catgccgagc attgtatcca tgcatgaaga aggcagaaca ccgcagggga tttttgacct
    13981 ggcgggcaat gtttacgaat ggacccgcga tccctggcgc ccctattgcg cccgtccgga
    14041 cagcgccgaa gctgcagaag tacgtcccag aaaagccatt agaggcggat gttatgagtc
    14101 tgctgcggca gaaatgacta cgagttggcg caaaggcgag tttcataatg tccggctggc
    14161 tactttaggt tttcgctgcg tgataccgct gtagctgtag tcggttttcc ctgattttct
    14221 tccttttatg acgctttctt tcggagaggg aaataaacgc cgggggcttt cgtgttacac
    14281 gggggacgtg aaagaagggc ggtagtgcgt gccaaatcag aagacgggcg tttgcacgct
    14341 cttttgaatt ttcgggagag tggtcaagtg cggaaaaaga atttcgttac tgttttcagt
    14401 cttggggtat gcctgctttg catccccgtg ctgaccagct ttctggatcg taaagcaccc
    14461 gatccggtac atgctttcaa agtgcatgtg aatgaggggc gggttgtcct gacgtggata
    14521 aacccggccg gaaatgactg ggcgggggtg ttggtctgcc gtaaagccgg ggagcctccc
    14581 aaagagcccc tggacggttt tgtgcttgtt tccggtgccg ttgaccgtgc ggaagatgtc
    14641 gaagtgaccg gaggtgagac gtattactat gcggtatgga cctatgatgt gttagggaat
    14701 ttctctccgg ctgtccgggg ctttgcttct ctgccgtccg gtgtccgaca gcgcatggtt
    14761 gaaggggagc gcactgtgtc ccctcttacg gatacggtgc tgatgcaaaa gagcgggagg
    14821 ctgtcggcta tttccgccgg gacagtgcag tgcgcagtac tatcggctga cagcgtaaag
    14881 agcgctgccg gtgtgtaaac ctttcttcag cgggctgccc gggtatccac tgcgtcagtc
    14941 cgggcatgaa gcgaaccgga ttttatgata cccctttctt tttctgcgga gggcgtactg
    15001 ctctatccca gaaattaacc gctgctgctc ctgtaattgt gtgtttgttt catgctataa
    15061 tccgcatcgt tcaggacacg tattttaaag atgccagcag atcttcaggg tcaggtatat
    15121 ataaatgtgg agtagattta ccgaaagagc caaacatgtt atcgcgacag cccgggagga
    15181 agcggtacgt ctcggctgtg agtatgtgcg aaccgagcat atcctgcttg gtctgtgcaa
    15241 ggagcgggaa gggatagccg cccgggctct gaaaaacctc gacatcgata ttgacatgat
    15301 ggaagaggaa attgtgcggc tcactttttc cagtgccaac gccacgccta gtgaagaaat
    15361 cacttttaca ccgcgcgcca aaagggttct ggaactggca accacggaag cccatcgtgt
    15421 gagccacaat tacgtcggga cggagcacct gcttctcggt ctgcttcagg aaggcgaggg
    15481 acttgccgca aagatttttg aagatctgaa agtggatacc aagcgcctgc aggaagagat
    15541 ctttcgcctc atgagtaccc aggttcgtcc tgaaatgcag caggcgcagg ctcccgccaa
    15601 atcacaaacg gccgcactcg atgctttcag tcgcgatctc acccagatgg cgcgggacaa
    15661 caagctggat cctgtcattg gccgtgaaga tgaaatcgaa agagtgattc aagtgctgag
    15721 ccgccgcaca aaaaataatc ccgtgctgat cggcgaggca ggtgtgggca aaacagccat
    15781 tgtcgaaggg ctggcgcagg ccatagtaag cggtgatgtt ccggagctgc tgctcaaccg
    15841 gcgtgttctg gcgcttgatc ttgctggcat gatagcgggc accaaatacc gcggacaatt
    15901 tgaagaacgt atgaaagccg tgatgcggga aattcgcaaa gcggacaata ttatcctttt
    15961 cattgacgaa cttcacacgc ttgtgggtgc gggtgcggct gaaggggcgg tggatgcggc
    16021 gaatatgctg aaaccggcgt tgtcccgggg ggaaattcaa tgcgtgggtg ccaccaccat
    16081 ggatgagcac cgcaaatata tcgaaaaaga ctcggcactg gcacgccgat tccaaaccat
    16141 tctggttaac gcgccgaccg ttacggagac aatagaaatt atcaaagggc tccgggataa
    16201 atacgaggca caccaccggg ttcgttttac cgatgaagcc attattgcgg cggcgcagct
    16261 gtcaaatcag tatattactg accgttctct gccagacaaa gctgttgatg tgattgacga
    16321 agcaggcagc cgtgcccgtc ttcagctcac cacgcgccct gcttcattaa agcagatcag
    16381 cgtggacata gagcaggcga cccaggaaaa ggaagccgcc atcagcaatc aggagtatga
    16441 aaaagcggcg gcgcttcggg atcgtgagcg tacgctcatt gcgcggctgg aatcggaaaa
    16501 gcgcctgtgg gaacagaagc gggattccac tgatatggaa gttaccgcag aagacattgc
    16561 ctttgttgtt tccaagtgga caggtgttcc gctgaccaaa ctggaagaaa aagaatcagc
    16621 gcgcctgctg cgtatgcgcg atgaactgca taaaacagta gtcggccaga atgaagccat
    16681 cgatgcgatt acccgcgcca ttcagcgctc ccgtgctggt ttgcgtggtc gcacacgtcc
    16741 ggtagggtct tttctgatgc tcggccctac aggcgtagga aaaacactgc tgggtaaagc
    16801 gcttgccgag tttttattcg gcaatcagga agcactgatt acggtggaca tgtccgaatt
    16861 catggaaaag ttttccgtgt cacgcctgat cggtgcgcct cccgggtatg tcggttatga
    16921 tcaggggggg caattgacgg agcaggtgcg ccgccgccct tattctgtgg tgctttttga
    16981 tgaaattgaa aaagcgcacc ccgatatttt caatgcgctg cttcaggtgc tggaagaagg
    17041 gcatttaaca gacagtgccg gaagaaaagt tgattttcgc aacacagtca tcatgatgac
    17101 ttccaatata ggcgcgcgcc gtatcggcaa aaacacctct ctgggctttt accgggacac
    17161 agccgatgaa aattatgaac ggatgaaaga gcgggtcatg gaagaggtgc agaaaatctt
    17221 caacccggaa tttctgaacc gtattgatga agtgctggtg ttccaccgtc ttgggcatga
    17281 ggaattgctt gaaattgtgg acttgcaggt gaatgaaatt gttgcccggg ttgcggagca
    17341 gtccatctgc ctcgaagtga cgccggaagc ccgtgaattt ctgttgcgta tgggcagcaa
    17401 tgaagaatac ggcgcccgtc cgctgcgtcg tgcgatccag caatatgtgg aagatcccct
    17461 ggcggaaatg ttattaaaag ggaatttaag tccggatacg gctattgtca tgcgtccttc
    17521 cgatgaaggg aacgcattgg tacctgaggc catggctgaa accacgggag gtatgctctc
    17581 ttgaatacga agtctttgtt tacaccgctt gcggtccttc tgttgatctc cggcatggtt
    17641 tgcgtcggca ttgccggcgc acaggaaggg gaagcacgga tcatccggga tatcagcttt
    17701 gacgggctgc tgcgcatcag cgatgctgcc gcccgtgcac agctggaaac gcaggtcggt
    17761 gccgcttatg atgccggtgc agttgcccgc gacatcaagc gcctcatgga tcttgggttt
    17821 tttgatgccg tcgacgcaaa agtcgaagag gcgggcggtg agataatcct tacctatgcc
    17881 gtcgtcgaaa aaaggctgat tgaccagatc cttattgtcg gcaacagaaa gattaaaagc
    17941 cgcgtcatcc gcgcagccct gacgatgcgt gagggtggtt cttttgagcc ggaaatgttc
    18001 gaaaatgaac ggcaggcggt gcttgatctc tataagggaa aaggatttgc gaatacgact
    18061 gttgacgtaa cggcggagaa tatcggaccc tcccgtgtgc gccttgtgta caccatcggc
    18121 gaaggcaaaa aagcacggat taaaagcatt acctttgtcg gcaatgatca tatgaaaacc
    18181 aagcgactgc gtaaggtcat gaaaaccaag caggcgcggt ggtttttcag cagccgttat
    18241 gacgaagaca aatttgcggc ggatctgacc aatatcaccg atgcctatgg tgaacttggt
    18301 tttctggaag ccaaagttgc ctccacagat atggaatatt ccaataacgg caagcgcatt
    18361 gatataacga tgtatctgga ggaaggccct cagtactcca tgaactctct ggatatagcc
    18421 cgtaatattg tttatgacga cgaggatctg ctggatgtcg tcaaagtgca tccgggcgat
    18481 gtgcacaata aagtgcaagt ttccaaagac gttgcagcaa tccagcaggg ctatcaggac
    18541 agcggctata ttgatgcggt cgtgacccct cagattacgc tggaccggga aaacaaaacg
    18601 acgaatgttg tttaccatgt ggaagaaggt aatctgaaat atctgcgtga aatcaagatc
    18661 accggcaatg acatcaccaa agacgaggtg atccgccgcc agatgctgct catccccggc
    18721 gaacgctacg atggcgcagc ggtcaccgaa agtgaaaacc gcattcagaa cacacgcttc
    18781 tttgataaag tacgcattac ctttgatgag accgacgacg aattgtttac ggatctgcgt
    18841 gtgaatgttg aagaaggcaa aaccggcgtt ttcaatttcg gcgccggata cagtacggaa
    18901 gacaggctgg gcgtctatac ggaaatgcgg ctcagcaatt tcgacctttt caactggccg
    18961 cgtttttcag gtgccggtca ggtacttcgg ctgcgcaccc aaatcggtga acggcgcaat
    19021 gaatacagca tcagcttcac cgaacccgaa tttctggggt atccggtgtc tttcggtgtg
    19081 gatctgtttg acgaatccta tgaagtgcgc ggtgccgcac gatacaaaga agagatccgg
    19141 ggcggtcagc tgcggctggg caaatccctt tccccctatg tcttcgccca gtctatctgg
    19201 cggttccagg aaacggatct gagccagttc tcccggtggg tgcatccgga aatcagaaag
    19261 caggaaggac gttccacgac cattgccaac cgctggcaga tcgagcgcaa tacgctggat
    19321 tccaattttg atcctacccg cggttccgtc catgtgctgg cggcagaaat cgccggtttc
    19381 ggcggagacc ataatttcct gcgtttcgag caggattcca cctggtaccg tgcgctgggc
    19441 gaaaaagaaa agatcgtcct gtcattgcgc gcccgtcacg gtattatgac cacctacgga
    19501 agttccgatt acattcccct gcaggagcgt ttttatgccg gcggtacgac gactgtgcgc
    19561 ggctacagaa accgggacat cgggccgaaa gttcgtgagt accggtggtg gggcgatcat
    19621 ttcgccgtgg gcggtgatac acgctttgtt tacaatgcgg aagtcaaata ccgtgttacc
    19681 gacattttca ggctttacgg ctttgccgat gcgggtgccg tatgggcgga tgcgggtgac
    19741 atcgatttcg gcgactttaa ctacagcgtc ggtgccggtg tcggcttcaa cgtgcctatg
    19801 ttcgggccca ttcgcgtaga ctacggctat cccctcaacc ccaacaaaaa tcagagcagc
    19861 agcgggcgcc ttcatatgtc cacggggttc cgcttctgag tttcgggagt gcgccgcctg
    19921 tcggtgcacg ccacagactg tatgcagggc agcgtttcgg cgctgccctg tttctttttt
    19981 ggaaaccggt cagttgccgg ggatatgaag cgtttcgccg atgcgtatca cgtcgctgtt
    20041 tttgttgttg tactgccgga ttgtttccgt tgtagttccg tatcgcacag caagtttgga
    20101 aagagtatct cccgcagcaa ctttgtgctg aattatggtt tcctgtccgg aaccgggctg
    20161 ccggggcgtt tgggttgcga cggctgtggc tgcttcgggc tggatgtcga cggggaaaat
    20221 acgggaggaa aacctgtcaa gccgcttcct gctctgacgc agcatatcaa ggaactgagg
    20281 atcgttttcc gcttcaagcg caatggtgcg gacgattttg cgcaggagag cggggttgtg
    20341 ctcgcagata attacagcat cacagccggc acggagcgac cggacaacca gatcttccag
    20401 agcgcctttt actgctttca tattcatgtc atcggtcata ataaagccat cgtagcccca
    20461 gtgttctcgc aggataccgg tcaccatggc acgggaaagg gtcgcaggag tatcaggaaa
    20521 ttccggatcc agtgcaggac aggcgatatg ccccacaagc acagcaggca cggttgcgtc
    20581 aacggcattt ttaaagggga gaagcgtatc ttccaatgcc tgtcccgtta ggttcaggtg
    20641 tgccagctgg acatgtgaat cttcaatggc ggcgccatga ccgggaaaat gtttcaccac
    20701 aggcagcagc ccttcctgaa ggatcccgtc catgaagcgt tttccaaaga gccaggcgat
    20761 ttccggatcg tccgaaaacg ccctgtcgcc gatggcacgg ctgcccggca ctttcagatc
    20821 caggaccggc gcaatcacta cttcaatacc gcgctcccgg gcggcgcggg cataggcaat
    20881 ggctgtgtcg cggatcttgc cttcgctccc ttcagcgcca agagacgagg cagagggcgc
    20941 attgtccaga cgcagccggt tcactctgcc cccttcctga tccacataaa ttaagggaag
    21001 ggcccctgag aaggccttgc cgctgacagt ccttttaatg gcagcaacga gatttaaagt
    21061 ctgctggcgg ttgctgatat tgttcccgag gagaagcacg ccgcctggct gaatctcttt
    21121 caacaagtcc cgatcctgcg ctaaaagcgt ttttcccggc agcgcaatga tgaggctgtg
    21181 ccccgggatg attttctgag cctgagcagc gccacatgta atggattgca ggaggaaaag
    21241 agtaatggct gtctggataa aaactttcaa tgctgcgctc catggttcag ggaagatggg
    21301 ggaaatgcgg actccatcga aaaatacccg gacatcatag cccctgtgcc cggtgccgtt
    21361 ccagtaccag gagacgaaaa gtccgaagga gcgacataaa aagaaaactc aggaatatca
    21421 gggcatttca gccaggcggc tttttgcaaa ctcaaaatac gccggaacga tttcgaaacc
    21481 gatgtaatcc cgtccggatt cacggctgat gacagccacc tgtctggaac ccaaaaacgg
    21541 atcgagcacc agatcccccg gctccgatgt ataggcgagg attttccgga tcagctcgcc
    21601 cgggagtttg gttggcgtct tcatgtcacc gtgccagtat tcccgctgga tagtccagac
    21661 gtcctccaga tccgcataac gcttgcttct gcctgtcgct gtccggtcat cagaactgaa
    21721 ccgggcattg tgaaaaaact tgcgttgctt gttgtcttta cagagataaa ggcaatggta
    21781 gtgggaggtc acaaaacggt atttgcagac cacaccgaac tgatatttcc agataagatg
    21841 gtttactgta atcagattga gtgcatcggc agtcaccaga atatctttca gattattcca
    21901 gccggaaaag ataaaagccg cgcctgactc tttgaggacg cggcgtattt gtgtcatcca
    21961 gttccgtgta aagtcgggat aatcgactgt ttttacttcc tgatacccgc ccagaaccag
    22021 atgagggtgg cgcttgtaat tgtctctcag cggtgtgaaa tcaatggcaa agggaggatc
    22081 cgtaatcacc agatcaacag actcgtcagg cagccgcttc agaccggtaa gacagtcctc
    22141 attgtaaatc tgattacgtt cccatccact gtgcatgtat ggctttcatg attcagttgc
    22201 cgggattttc aataggcacc cagcagtaag gctcttcttt ggtttcatca taacgataat
    22261 acccgccctt tttccagatg aagaggatct gcaccgcatg cttcatttct cgggtgccct
    22321 tctgccattg ggaaatatag ctcagcgctt cgttttgcga aaaatgtccg tcattatcag
    22381 tgtcggcagg atggcgtggc tccggctctc cttcgacttc cgcctctgtc tcaaccctgc
    22441 cacggaactg gatgtcgtca atattccatc caggataagt ttgagaataa tcggtaggcc
    22501 ccataaccca tcttacccgg acattgggct gcccgtccgc aacagcggag aggttatatt
    22561 cgcattcaac ccattctgta tccagcactt catcccgggc tggattcttc cagacagtga
    22621 tccaggtgta accgttgtta ctgacttcaa cggctgcttc gtcgtactgt ccctcttcaa
    22681 cgcccagcca gcgcataaaa cggagggtca cgccggatgc ccgggaaaag tccagggatg
    22741 gtgttgtcag actccgccgg ggaagatcac tttcataatc gcccttaagg ttgtagccgt
    22801 atacatactg cccggtataa gcccgtgatg gatcgcggtt ataggagccc agtcccgcag
    22861 ggcgaccgaa tgcccatccg gtatcagcgc tccagccggg gttgctgtcc agcggaaagt
    22921 tgtaaatgac cggcggcgtt aatgtgagtg ccgccggcac agactgtacg gaagcactgc
    22981 tctgattggt cacgaaaatc gttccgctat agtttccggg ctgcagattg actgtgtcga
    23041 gtgcaagtgt cagagagagg gtgacagaag tgccgggggc gatggtgcct tggttggcgg
    23101 agagcttcaa ccaggtgggc gctgttgcgc tccaggagaa gggcgcagtt cctttgtttt
    23161 ccaggagcaa tgtgttctga gccgatgcga tgctgctgct gctgcctgcc tctgcggaaa
    23221 aagagagatc ccggggagag acgctcagat catcaccgta gatcacccag ctggcgctgg
    23281 agccgatcag gtcgacgccg tattccatat agccgtagcc gcccattccc cagcctgttc
    23341 cccaggagtt ttggacgagc caggcgccct tggaatcatc ccagccgata atgatgacgg
    23401 catgatccgg gacattccac gccttctcgt cttttttgtc gttataaacg ccgccggtat
    23461 aggcaatgag ggacttggag attttgagat aggtaccgag ggggccgtat tgatacagcg
    23521 ccgctttgat atcctctacc ggggcgatat tcaaaaaagt gccaagctgg ctataacttt
    23581 tgatccaata cttgtgcgga tagggacatt tgcatgggct gtcccatccg gtatagggac
    23641 aatccttttc cagaacagcg ccggagctgc cgcagtcatc ctttttgtct ttgaaatagc
    23701 ccaactgcat gaaaccgccc gtggaacagg tatgagccag gatttctgag ttgcagctga
    23761 tcagccactg ttccgagagg tcgacgactg tgtcgtcatg aattttaata gcccattcca
    23821 gcacttctgt tgtggcaaat gcccagcagg atccgcagct cccctgatct ttcacaggcg
    23881 gcaggggcct tgagacatgg tcgcgccagt caaagcggga agggagtccc gctttgttca
    23941 gcagcgccgg atccatctgg tgttcttctt cttcccgcaa ggcttcttca gtaaaacgga
    24001 cgttgcccag ttgttccaga ggccgctgag atgcttccgt cagcccgaca gagaaagtcc
    24061 agcctttttc cttgccctgc tgctgcaggt ctgcaatatc ttcgggtgtc agttgaccga
    24121 acgcgaagtg acttacgctg atgtatagaa aaagcacata gagtacgtgg tatttgctta
    24181 aggtcgttgt ttttttagaa atagcgggca tgattcactc ctattttatg ccccttgggt
    24241 actttctaca tggtatcata aactaagaaa tgaatcaaca cttcgcaaag cgcaaaaatc
    24301 aataaaacgt caatgcgaac ttaggagaca ccaggatttc ggcgcgaatc attcagacgg
    24361 acagcgcatc cagttctaat tcgaaaagga gatcgtcccg gcagatatcg gtgtgtgcca
    24421 gcaaggcggg gaagggagag attccgcggg cagcgagaca tttctgaaac agaggggcat
    24481 cggcaggatc tgtgaa
//
LOCUS       DAOPWB010000037        24057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2075_length_24057_cov_6.521582, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000037 DAOPWB010000000
VERSION     DAOPWB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 24057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24057
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2075_length_24057_cov_6.521582"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..1107
                     /locus_tag="PKN92_05200"
     CDS             505..1107
                     /locus_tag="PKN92_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ48221.1"
                     /translation="MRAKKTNTEIRKEQILLAALKIIGSQGVNALSIVGVAEKVGIVP
                     SALYRHFSSKEEILDGILDLMREKVLKNVQQARLKKHNALARLEFIMMRHLALLKESR
                     ALPLILLSDAMYLGTSARLSKIQEILESYLSALSAILKEGMEEGSIRADIVPESTAIM
                     FLGMILPAAVLVNVQAPFFNLESHAANVWPAFVRSIAVIN"
     gene            1130..2296
                     /locus_tag="PKN92_05205"
     CDS             1130..2296
                     /locus_tag="PKN92_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ48222.1"
                     /translation="MSKKKRLLVLFVAVSAAGLFAYFYNEPQLFRQESEHVLRLSGNI
                     EVREAELSFKIPGRVIERIAEEGQLLSTDVVAAKLDSVDLEQQLALRKAELHAAQAAL
                     AELEAGFLPEEIAQMEAAVSKAQARLDELLAGSRPEEIASAKAAVASAGAVEEHMRIE
                     LERQSRLLASETISQREYDKAISEYLGASARLKSANEALKLVQVGPRTEQIAQARAAL
                     TEAEQQLALMKKGPRQEKIEQARARVEQASAACDFARTQVSHTLLYAPFSGMVLSENV
                     EVGEYVSPGTPVITLGDLENTWLRAYIAESDLGKVKYGQKVHITTDSHPGKIYTGHIS
                     FIASESEFTPKTVQTQKERVKLVYRIKVDVPNPELELKPGMPVDAAIQFEETHK"
     gene            2296..3246
                     /locus_tag="PKN92_05210"
     CDS             2296..3246
                     /locus_tag="PKN92_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48223.1"
                     /translation="MAAVRTDNLSKEFNGMQAVCRLNLEVQPGEIFGLVGPDGAGKTT
                     IMRLLTSIMEPSSGDAWVQGLHSVRDAEAIKEKIGYMSQRFGLYADLTVQENVDFYAD
                     IYGVPRRGREKTIDELLSFSALTPFKKRFAGNLSGGMKQKLGLACALIHTPEVLFLDE
                     PTNGVDPVSRRDFWRVLYQLRQKGVTIVVSTAYLDEAERCSRLGLLHQGNLLSVGSPR
                     EIKSLMKGQLLVVRSSHPREAAQLLQEVLREEHITLFGDRIHVITDAPESSRTSITHT
                     LSGSAYTLHSISDQEPSLEDVFVSMLGTTEKERSHEIVSS"
     gene            3227..4201
                     /locus_tag="PKN92_05215"
     CDS             3227..4201
                     /locus_tag="PKN92_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48224.1"
                     /translation="MKSSPHDIAVSVKNLERRFGSFIAVDRISFEVSRGEIFGFLGPN
                     GAGKSTTIRMLCGLLAPTGGSGTVAGFDLRKEAEQIKSNIGYMSQKFSLYDDLTVEEN
                     INFYSGIYNIPKNKKLERKHWVLEMAGLTDQINLRTGFLSAGWKQRLALGCAVLHEPP
                     VLFLDEPTSGVDPISRRQFWTLIYTLSGTGVTVFVTTHYMEEAEYCDRLGMVYRGELI
                     ALGAPEEIKTQMVDDRIFEIQCAHPEKLLAKMPEFPEFSDAFLFSNTLHVITNDRNLT
                     AENIRTRIEQEGWNISRVEKILPSMEHAFVSLIEKRDRTENAREAVKR"
     gene            4198..5331
                     /locus_tag="PKN92_05220"
     CDS             4198..5331
                     /locus_tag="PKN92_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ48225.1"
                     /translation="MKLMRIRAVSRKEFIHILRDPRSLGMAVAIPMLLLLLFGYALTL
                     DVDNVPLVVWAQEDSPIVQDLVRRFSASPYFSLQGEVGSYEELEYAIDSRTALIALVI
                     PAGFSGRIESGRTAPVQGIVDGSDSNTASIAISYAQAVTAAYSQDIALRTFQRMGERT
                     ADLPVQVEARVWFNDDRESKNYIIPGLIAVIMMVIAALLTSLTIAREWEQGTMEQLIS
                     TPVKAPELVIGKLFPYFVIGMVDVLLSVLMGEYLFHVPLHGSIPLLFAMAAVFLMGAL
                     SLGILISIVTKSQLVASQLAMVLTFLPAFLLSGFMYAISNMPRAVQLLTYLIPARYFV
                     ALLKGIYLKGTGLAVLYQEIILLSVFGLLMFLLAIAKFKKKLV"
     gene            5335..6465
                     /locus_tag="PKN92_05225"
     CDS             5335..6465
                     /locus_tag="PKN92_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ48226.1"
                     /translation="MVARVTHMLIKEFIQIFRDPRLKGIIFLMPMIQLLVFGYAVTTD
                     VNQVATAVYDLDNSSASRELVAAFSDSGYFNVVEHISHPDRIHQVLDHGDIQAVLQIN
                     SGFSGNIESGRSTRVQLIVDGTDSNTAGIVMNYSSKITGALSREKLANRLSRMKGRMP
                     EIPEVTLEPRAWFNENLESRNFYIPGVIAIIAMLITLMLTSMAVVREKEVGTMEQLMV
                     SPIRPVELVLGKTIPFAFIGFADVLLITVLGVFWFNVPIRGNLFLLFFSTGLYLMTTL
                     GIGLLISTVSRTQQQAMMSTFFFYFPAVLLSGFMFPIANMPTVIQWLTLLNPLRHFLI
                     IARGIFLKGVGLPILWPQLVFLLIMGSAVLWLATRRFHKTLE"
     gene            6485..7924
                     /locus_tag="PKN92_05230"
     CDS             6485..7924
                     /locus_tag="PKN92_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ48227.1"
                     /translation="MTHRSFSLRISAMLLLVLSSSCSTVTSDSIARIENYYPKPAEEN
                     MLPVKNGPAPEESVSPVPEQRALTLEHCVAIALDRNPLRKAAEEGIAVARANTNAVAS
                     QYYPSLTWSASYRRWESHAFLPSDLGLPGKKPSSIGPVSDWSTGLKAQYLIFDSGKRA
                     AELRAAKSAVAAVSEEAEKLQQDIALSVHHAFYQLCAAREGYATAEQNKKRAEAHTAL
                     AARLWESGAVPKADVIRAQVDLSESALKLVQFETAVKEARVNLNRALGLPLEMEFALQ
                     TDMPVLTDPDTIDMNTAVGQALHQRPELKAALHLIDQQQHLLSGAESAFGPTVAASLG
                     YGYRDDSFPVKDKDWSAGIGIELPIFDGFRRKNNVAKEKHAFARQEALLRQKVLEISE
                     EIWVSKLHWAESFAAIALSEAIVHDAEESMRMIRERYEAGAATATDLLDGQSKLVGAQ
                     FGLVEAHWRFFAAKARFDHACGNLVAASD"
     gene            7989..9350
                     /locus_tag="PKN92_05235"
     CDS             7989..9350
                     /locus_tag="PKN92_05235"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HNZ48228.1"
                     /translation="MMHLWKKSTLFSLGLILLAPFAVSYGDASTTGTPRLMEGPMVGA
                     VSSTEISVWVRASGEFPLALEYDQKADFKSSKRTEKVVPSIEKDDRTAVLRMSGLEAD
                     TLYHYRILINDKPAPYIGAFCARTAPAGPARFTVSFGSCPRYQQDPVQNIWKGLEKVA
                     PDLFFWLGDNIYGDTFDPDILAECYRYQRAVPNSRHFLATTPQLAIWDDHDFSLNNHN
                     RTNPIKEASLGVFKRYWANPSYGLPETPGVFFKYHYGGVDFFFLDVRYHRDPNTEPDS
                     PEKTHLGKEQLAWLKEELLASRTPFKVLVSGGGFSNEKGPKGDSWASFLYERNNLFDF
                     IRDREISGVLCFSGDTHVGEFNAIPWSEKGGYDFYDLDSSPLAQKASDNWKQRTPEIR
                     LRMPYQANNAGLVEFDMTAEPPTVRLNLMTETGEPVWDPVVLTSDDLKNGTATAEANI
                     KLD"
     gene            9405..10592
                     /locus_tag="PKN92_05240"
     CDS             9405..10592
                     /locus_tag="PKN92_05240"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNZ48229.1"
                     /translation="MSENIMEPSSEPANAAEESPAAVQQKQEQKPVTVKPERMMSIDA
                     LRGFDMFWIVGGQGLVVAFLLLFSASGELPAWLDYQFSHVDWVGFSAWDLIMPLFLFI
                     SGASIPFAMHLKEGETRDYKAFYVRLARRIALLWIFGMIAQGRLLTLDWTQFRFFSNT
                     LQAIAVGYLIAVLAHLYLPTKGQIILFMDVLIIYWGLMMFVPVPGADGWTLEPRLNLA
                     LWLDDTILGRFRDGTTYAWILPGLGFAGSVMLGVFSGMILRLKEAPMKRFAYLLGLGV
                     ACLVGGWLWSFHFPIIKHIWTSSMVLWAGGWSCLLLALFYLVIDIWKFQRWAYPFMII
                     GANAIAAYMAEHLIPFKQLALLIPGLACECPYAAFFASFIAFMLLWIPLWIMYRYKIF
                     LRV"
     gene            10658..11635
                     /locus_tag="PKN92_05245"
     CDS             10658..11635
                     /locus_tag="PKN92_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HNZ48230.1"
                     /translation="MTSFLEGLRAWRALNHRKEQPEDPETHAGALAENFLKRLIDSNL
                     KFNNGYAFPGKRIPSHIYGNRYEVDLIVLTPKYIHVIEVKNWSGTLQLQDGQWVQTRR
                     NGQEIQHASLSSLNQEKKEALIAYLHNRSIPVNPSQVTQKIIFMNNNLRILSPEIRKD
                     PHIIQAYELKHYLDSQWKSASDEKIVQSIIEVLLAKEKQEKAFSRSVQGKYFYATLFL
                     LRSLETWDRLRLHGGKTLVGDGLQLITHRRSFDLKKLPSGTKYPVSWSQPSPLLYLLC
                     LLGKSTVGSINLPNRKIAISPHDMIKFHQAMATKPSVIPLGEVDLFIRG"
     gene            complement(11654..12601)
                     /locus_tag="PKN92_05250"
     CDS             complement(11654..12601)
                     /locus_tag="PKN92_05250"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNZ48231.1"
                     /translation="MKSVLVTGAAGFIGSHLCDALCARGDRVIGLDNFNDYYSPAVKR
                     NNIRDLLSQKNFRLYEKDICDEEALDQIFNQERPDVVVHLAARAGVRPSLEDPNLYHR
                     VNVIGGQHMLDMCRKYTPSHLVFASSSSVYGGSKEVPFKETDPVMRPISPYAATKRMN
                     ELQAHVYSHLYGVKVAMLRFFTVYGPRQRPDMAIHKFARLLLEGKPIPMFGKGDTRRD
                     YTYIDDIIQGVLGCVDRPFSYEIFNLGEQHTTGLSELIALLEKQTGCKAKIEEKSLQP
                     GDVEITYADISHARALLDYNPRFTMDEGIARFVHWLKSQ"
     gene            complement(12614..14467)
                     /locus_tag="PKN92_05255"
     CDS             complement(12614..14467)
                     /locus_tag="PKN92_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48232.1"
                     /translation="MTVQRLHRFQIGGRHFVLDTHNCFCFECDEITGAVLELYPQESA
                     RRIMTLLKDRFPEAEVQEVIGELDYLRVTRSILVPGNDEEWLKIVHQPAEVTCLTLCS
                     STDGTAALVRDCLPFLLGRSGTQQDLTIILRCTGGGKGLDEEALNTLQYAADAVLSAG
                     KKLSFQLDVPIVTKDLQHPACSFRVHLPIAGVGAIKSVREVLWAQNTLKSRVFLERLE
                     ALEGACPLQLIIQPQTASFSDAFKQAVEAGFKNIRLDLPFLWMHQPAHDPDEVAAQLI
                     KAAQDYSAMLLRQENLYFYPVSDLFKAIRDGEPCYRTDPSGFSALAIGEDRQIYPSVD
                     FMTARSFALGDLAAHCMNESIYEQFQQKSTLTCAECVRCWAQGLCGGGYSAVHAARIG
                     DPFRPEEQWCAAQRQWTQHAVALFTPLASLPLSFDPPLSTHKTSAPKIKWGAALKALF
                     TEQILARPLQESDADWRVRWENWNDAAFFLCAETNVQTTTLYDKEMDATHPRSGAQEY
                     VLTDKSGKPCGLLKIAPDPIHHLAHIWLYMHDPASYGKSALHTVLGTLLNQMASVPGI
                     RHLIAYASAREQELMAALEALSFVSAGTQRDALYHRGQYEPLHLFTYRISE"
     gene            complement(14467..15327)
                     /locus_tag="PKN92_05260"
     CDS             complement(14467..15327)
                     /locus_tag="PKN92_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ48233.1"
                     /translation="MSFLLVMKAELVRSFIIMRRYWFRTITGLVLGYGMLMVLVAGFI
                     YSREGLEERVQQFDDPSSATNFILGFIVGMFAFGVVGMFTQGLQGMAATGVLEQLCMS
                     PHGLAVNFMAQTVVSSVATILSSGIMVYAVTWSVGGMLHWDTIPVLILLTLTFFNLVG
                     FGFMVGGLVLVFKQVGQVAILVRMGLFALAIFAKEELLGNPWPFTFLLHVLPITDATI
                     CIKYVLIKGQMRDISTAGAAPEMVFSSVFAHSSFYWLIGNCLFWTFVGLTLFKLMEDY
                     SRSKGTLGSY"
     gene            complement(15312..16373)
                     /locus_tag="PKN92_05265"
     CDS             complement(15312..16373)
                     /locus_tag="PKN92_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48234.1"
                     /translation="MTDTALVELRGVCKEFSTSHVLPVDEETRKRRLFKKKVVCAADN
                     ISFSIAPGEIFGLLGPNGAGKTTAVKMISGLVRPDAGQAFVKGLNVEKKRRQILRHVG
                     VVLEGTRTSLWPLTPFENLVYYGMLKDVRGSVLKERAHDLLDFIGLQDKKNVQVRRLS
                     RGQKQKLAICIAMIADPLVLLLDEPTTGLDVQSSRAIKDKVREITREQGRSVLVTTHD
                     MHVAQELCDRIAIINHGKLIACRPTDSLLEVFSEQVFAFRLDRRVPLDLFSGIPGIIK
                     SEYEGSEEPELVFQLVGEQQDLRSEALYKIIRIFEEQKCLLRSVSQRKQNLESIFLRL
                     TSDDPDADVLKKGNTCLSS"
     gene            complement(16370..17065)
                     /locus_tag="PKN92_05270"
     CDS             complement(16370..17065)
                     /locus_tag="PKN92_05270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNZ48235.1"
                     /translation="MNILFLSLWLVHTGISGFKHVRKIPLVFFPDTVLQGIVLSVAAV
                     WAWNQALLTRYIWSPFDISLGIVTGHVLFFFALLITHRQPGDVFRLFLSFRDIFRFVA
                     RAPLLCVRLLGLCLVEELVYRVAGQSILIQLLPASWLAVILTAVFFSVMHGHFFRSGW
                     VSAIEFFLFSLVIGALYAFTWSISIVVFVHFIRNLESTYLDYVSLVQDGIAPEDAVKT
                     IENSQNNLVLEAS"
     gene            complement(17062..17688)
                     /locus_tag="PKN92_05275"
     CDS             complement(17062..17688)
                     /locus_tag="PKN92_05275"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNZ48236.1"
                     /translation="MKKGLHSFLRRAEREDLDSILAWMEDPEFQYFLYGDEAQSQRQI
                     REKIILMLGRTPPAAPPSAIHLLIDSKEEGLLGMIGLQNISWRNRSCSLDIYIGARSY
                     RSSILPAVSIFRALEYVFDELNLHRVSSLIYAFNERSWRMMEKSGAKRELVLREHIYR
                     NKTLHDAYGYGLLRHEFYALREKLGAVSGSISLDAMIKAMADDSGDTK"
     gene            complement(17685..18248)
                     /locus_tag="PKN92_05280"
     CDS             complement(17685..18248)
                     /locus_tag="PKN92_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48237.1"
                     /translation="MKLSPHTTVRFCEPDDADFIRDLYYDGIPRAALLDGRREPMLPL
                     RSEILEIIRKSEAARAMLFSIEDGTGILRAWSGLRGLNPDARFAELFLVFADEAGYDS
                     LQADEALGYLLDRAFFQTDLGRVLVSCLESEKAWRQFLLRKGFSSCGIQRDIMCISGT
                     WTGIETLELTAETFYKQASQDKGGEKS"
     gene            complement(18350..19732)
                     /gene="mnmE"
                     /locus_tag="PKN92_05285"
     CDS             complement(18350..19732)
                     /gene="mnmE"
                     /locus_tag="PKN92_05285"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNZ48238.1"
                     /translation="MSFLFTEDTIAAISTPPGEGGVGIVRLSGTESLRIASRIFRSSR
                     GKDIRARGQRVFHGHIIDAAGFPVDEVLLHVMHAPHSYTAEDVVEINGHGGRASLQAI
                     LDTALAQGARLAAPGEFTRRAFVNGRIDLTRAEAVMALIQAKSREALRLAENAASGRL
                     SERLQALQQQLKHCLAHAEASVDFPEEDLPELITPVFFTNLEQVSREMRELLASAETG
                     LLLQEGVSLAIVGQPNVGKSSLFNVLLRDARAIVSPEPGTTRDHIQEVLTLGGIPIRL
                     CDTAGLRDTQHHIESQGIARSRQAARRAALLLFVADASVGADEEEKRLAAEMLQLGVP
                     VVLVLNKIDLAPLHERNFPDIPFAAVCPVSAVTREGIDGLEKTLVTLLAGDLDAALDA
                     PALFRSHQKESMQRALACVQRVLEHPSASPEILALDLREAVHALGGITGETTAEEVLD
                     LIFNEFCIGK"
     gene            complement(19729..20955)
                     /gene="jag"
                     /locus_tag="PKN92_05290"
     CDS             complement(19729..20955)
                     /gene="jag"
                     /locus_tag="PKN92_05290"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNZ48239.1"
                     /translation="MKIIESSGQTREEAIQNGLTELGVAMDEVERIDIIDEGSKGFLG
                     MGKRPVRVRLTVDMAAISSNKSQGRQERRHDSRRDRNTAEKASQGGARNEQERQAPRD
                     GAQDRQSKRAKGQSQPQNQRDQQNRRNGQNRASGDQNRRNKQKRHASSEESRSKHRAE
                     KDQEAAVSLSGERQGSFAEKKKAASLIQEGTEATRLAEMFANNDDLQAVLTVEPVPEG
                     AHEEEAIEPISDAQGNEMAAMLKEIVDRMGLTADVRFIRDESDSPCLSMKSETDSAIL
                     IGKHGITLNALQYLVNRMLPSGDNENTERLMVDVGEYVSRRRAALVDMAKKMAKRAKE
                     TGRNVKLKPLSPQERRIVHLALEKDSDVRTYSQGDSLYRNIIINPVNIRRGGAPRPQR
                     SHPHRKQQNAASPGER"
     gene            complement(21026..22948)
                     /gene="yidC"
                     /locus_tag="PKN92_05295"
     CDS             complement(21026..22948)
                     /gene="yidC"
                     /locus_tag="PKN92_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNZ48240.1"
                     /translation="MLGDDRDRQLQRNQLLAIVLMTVLVFVWTYFFMPAAPPPAPPRQ
                     EDGTESTAEEERAGAGLFDRSDRTVAEENARTIPEGYPEIPPVASPPESPSDDELFLS
                     NNQLELTFTRVGARLKAATVLLGSNGQDSIQLVPDAEPADDAESVYPLSILFSDRALG
                     DQLNYCRWESVESSEPGVLVFRLEVPGYALLEKRFSLQPDSYVLETAIRYENWGEAAR
                     LLGLDRKEPALSLYWGPNVTSGDLTKGSQQNIVFHKEGKNTFHATAKMKPVNDGGWFN
                     ERLLDTDWAAISSAYFTVAIKPEFEFADSWYYGNKDYFRLGLGAPRKEVAPDEAAEFN
                     YSVYVGPRATQYLVQAWPGLDSVFSFFTSFSLMDRFAKLLLAILIWFHDHIIANYGLA
                     IIFLTILVRMVMFPLTWKSMISMKRMSQLAPEMEKLKAECGSDQQELQKRMMALYKER
                     GVSPLGGCLPMLLQMPVFIALYRMLWSAFELRRAPFIFWIQDLSEADRMLTLPFSIPM
                     PFSSVPLDSLNLLPILMAVSMVISQKMMPMSGPVQNQQQKMMMTFMPVFFGFITYNMA
                     SGLNLYIFVSTLLGIAQNYLVRGIKADAEPVKTKKPSRPRHFYDAARLKQREMNKEIR
                     KQKQRSRRRKDSGGRS"
     gene            complement(23000..23248)
                     /gene="yidD"
                     /locus_tag="PKN92_05300"
     CDS             complement(23000..23248)
                     /gene="yidD"
                     /locus_tag="PKN92_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015462597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNZ48241.1"
                     /translation="MSNILILMIRGYQKWISPLLGSHCRFVPSCSQYAIDALRVHGTL
                     KGGFLTLWRLLRCQPLCKGGYDPVPPSSKAFPQSRDPQ"
     gene            complement(23241..23594)
                     /gene="rnpA"
                     /locus_tag="PKN92_05305"
     CDS             complement(23241..23594)
                     /gene="rnpA"
                     /locus_tag="PKN92_05305"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNZ48242.1"
                     /translation="MCERLRFLRTQRLTARHEYDLVFSKGEKLWGRSYICYVLLDPSR
                     EARLGLVVSRKVGNAVVRNRVKRRLREFFRLNYFRLQPGMQLVIIARASGACQSSVAC
                     ASELNATLKRFLRHV"
     gene            complement(23605..23757)
                     /gene="rpmH"
                     /locus_tag="PKN92_05310"
     CDS             complement(23605..23757)
                     /gene="rpmH"
                     /locus_tag="PKN92_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNZ48243.1"
                     /translation="MKRTFQPSNTKRIRTHGFRARRATPGGRQVLASRRRKGRYRLTV
                     SDSIPR"
BASE COUNT         5878 a         6423 c         6003 g         5753 t
ORIGIN      
        1 atcctgaatt tctctgttga ccctttgcgg cgtcagtacc ccggtgctgg cgccgttttt
       61 tttgtgccac gaatacacga aaaaataaag agttaagtgg aagctccttt gtatatggaa
      121 gccaaggcag ccagaaagct cacgcccccg cctggttgtc agtgctcttt tctctttaaa
      181 ggattctgta cagtgcttgc acttcctgtg ccccagtacg ccctgtcaag ccatttgcta
      241 atcttaccgt agcggctttc gtcacgccat ttgctttctt actttggtca gatgctcttt
      301 atgtctttcc tgcctgtcct tgcgaaggat ggttgcggtt ctctgacact tgccggtcac
      361 ctgatttttt caaagcaggt gtatagattc cgagacatct attttgcact tcgtctgttt
      421 cgttattttt ttaaaaaagc ttgacttgcg ccatttcacc atgtataata gtgaatagcg
      481 attcactatt ctaaggagaa aagaatgcgg gcaaaaaaaa caaacacgga aatcagaaaa
      541 gaacagatac ttcttgccgc tctcaaaatc atcggctcgc agggggtcaa tgcgctcagt
      601 attgtcggtg ttgctgaaaa ggtgggaatc gtgccgtccg ctctttaccg tcatttttcc
      661 agcaaggaag aaattcttga cggtatcctg gatctgatgc gtgaaaaagt cctcaaaaac
      721 gtacagcagg cccgcttaaa aaagcataat gccctggctc gtctggagtt tattatgatg
      781 cggcatcttg cacttcttaa ggaaagccgt gctttgcctt taattcttct gtccgatgcc
      841 atgtatctgg gcacttcggc acgcctgagc aaaatacaag agatcctgga atcgtacctg
      901 agcgcactat ccgcaatact taaggaaggc atggaagaag ggtccattcg ggcagatatt
      961 gttccggaaa gtacagcaat catgtttctt ggcatgattt tgcctgctgc tgttctggtt
     1021 aatgttcaag ctcctttttt caacttagag agccatgcgg caaacgtgtg gcctgctttt
     1081 gtccgttcaa tcgctgtaat caactaattt ttatagggag aaacagttca tgtcaaagaa
     1141 aaaaaggctc cttgttcttt ttgtagctgt cagtgccgcc gggcttttcg cctatttcta
     1201 taatgagccc cagttgtttc gccaggaaag tgaacatgtc cttcgcctgt ccggcaacat
     1261 agaagttcgt gaagctgaac tcagcttcaa aattcccggg cgtgttatcg aacgcatcgc
     1321 agaagaaggg cagcttctct cgacagacgt ggtcgcagcg aaactggaca gcgtcgatct
     1381 cgaacagcag cttgccctgc gaaaggcgga actgcatgca gcccaggcgg ctcttgcaga
     1441 gttggaagcc ggttttcttc ctgaagaaat tgcgcaaatg gaagctgctg tcagtaaagc
     1501 gcaggcacgg ctggatgaat tgcttgccgg ttcccgtcct gaagaaattg cctcagccaa
     1561 agccgctgtt gcaagtgcgg gcgctgtgga agagcacatg cgcattgaat tggaaagaca
     1621 gtcgcggctt ctcgccagtg aaaccatttc tcagcgtgag tatgacaagg cgatctctga
     1681 atatttagga gcttccgccc gattgaaatc ggcaaacgag gctttgaaac tggttcaagt
     1741 cggaccccgc acggaacaga tagcccaggc gcgtgcggct ttgacggaag cggaacaaca
     1801 attggcgctg atgaaaaaag ggccccgcca ggaaaagatt gagcaggcgc gtgcgcgggt
     1861 tgagcaggca tcagctgcct gtgattttgc ccggacccag gtgagccata cccttcttta
     1921 tgcaccattt tccgggatgg ttttaagcga aaatgtggag gtgggagaat atgtatctcc
     1981 cggcacaccg gtgatcactc tgggagatct ggaaaacacg tggcttcgcg cctatattgc
     2041 ggaaagcgat ctgggtaaag tgaaatatgg acagaaagtg cacatcacaa cagacagcca
     2101 tccgggaaaa atttatacgg ggcacatatc tttcatagcg tctgaatctg aatttacgcc
     2161 gaaaacagtg caaacccaaa aagaacgggt gaaactggtc taccggatta aagtggatgt
     2221 tcccaatcct gaattggaac ttaaaccggg tatgccggtt gatgcggcaa ttcagtttga
     2281 ggagacccac aaataatggc agctgttcga accgataatc tcagcaaaga attcaacggc
     2341 atgcaggcag tctgccggtt gaatcttgag gtgcagccgg gtgaaatctt cggacttgtc
     2401 gggccggacg gtgcaggcaa gaccacgatc atgcggcttc tgacctcgat tatggagccg
     2461 agcagcggag acgcctgggt tcaagggctt cacagcgtgc gggatgcgga ggcgattaaa
     2521 gaaaaaattg gctatatgag tcagcgcttc ggcctttacg ctgaccttac ggtacaggag
     2581 aatgttgatt tctacgctga tatttacggc gtcccaagac ggggaagaga aaagaccata
     2641 gatgaactgc tctctttcag tgctctgacg ccttttaaaa aacgctttgc cggcaatctc
     2701 tccggcggca tgaaacaaaa actcggactc gcctgcgcac tgattcacac gccagaagtc
     2761 ctttttctgg atgagccgac caatggcgtt gaccctgtct cacggcgcga tttctggcgg
     2821 gttctttatc agctgcgcca gaaaggcgtt acaatcgttg tttcgacggc atatctggat
     2881 gaagcagagc ggtgcagccg gctggggctt ctgcaccagg gcaatctgct ctcagtaggc
     2941 agccccagag aaatcaaaag ccttatgaaa gggcagctgc tggttgtgcg ctcctcgcac
     3001 cctcgcgagg cggcacagct tttgcaggag gtactacggg aagagcatat cactcttttc
     3061 ggtgatcgca tccatgtgat tacggatgcc cctgaatcgt cacgcacctc cattacacac
     3121 actctttccg gaagtgcgta cacactccac agcatctccg accaggagcc ctccctggaa
     3181 gacgtatttg tttccatgct gggaacgaca gaaaaagaaa gaagccatga aatcgtctcc
     3241 tcatgatatt gcggtgtccg taaaaaatct ggagcggcgt tttggatcgt ttatcgctgt
     3301 agatcgcatc agttttgagg tgagccgcgg cgaaattttc ggatttctcg gccctaatgg
     3361 tgcgggaaag tcgactacca tcagaatgct ttgcggcttg ctggcgccta caggcggatc
     3421 cggaactgtc gctggatttg atctgcgcaa ggaagcggaa cagatcaaat ccaacatagg
     3481 atatatgagc cagaaattct ctctttacga tgatttaacg gtggaggaaa acattaattt
     3541 ttacagcggc atctacaaca ttccgaaaaa taagaagctt gagagaaaac actgggtgct
     3601 tgagatggca gggttgacgg atcagataaa ccttcgcacc ggatttctct ccgccggctg
     3661 gaagcagcgt ctcgctctgg gatgtgcggt gcttcacgaa ccgcccgttc tttttctgga
     3721 tgagcccact tccggagtag accctatcag cagacgccaa ttctggacgc tgatttacac
     3781 gctttccggc acaggcgtaa cggtctttgt aacaacgcat tacatggaag aggcggaata
     3841 ttgtgaccgt ctgggaatgg tttatcgtgg agagctgatc gcactgggcg cgcccgaaga
     3901 gatcaagaca cagatggttg acgaccgtat ttttgaaatt cagtgcgcgc atcctgagaa
     3961 gcttctcgcg aagatgccgg aatttcctga atttagcgat gccttccttt tcagcaacac
     4021 actgcatgta attacgaacg acaggaacct gaccgcagaa aatattcgca cccggatcga
     4081 gcaagagggc tggaacatca gcagggtgga aaaaatcctt ccttccatgg aacacgcctt
     4141 tgtttcactt atagaaaaac gtgaccgcac tgaaaatgcc agagaggctg ttaaaagatg
     4201 aaacttatga gaattcgtgc tgtgtcacga aaagaattca tacatatact gcgggatccg
     4261 cgcagcctgg gtatggcagt cgctattccc atgcttctct tactgctctt cggctatgca
     4321 ttgacgctgg atgtggacaa tgtccctctc gttgtgtggg cgcaggaaga ctcgcctatt
     4381 gtacaagacc tggtcaggcg cttcagtgct tccccgtatt tttccctgca gggcgaggtc
     4441 gggtcctatg aagaattgga atacgccatt gattcccgaa cagccctcat agccctggtg
     4501 attccagccg ggttcagcgg ccggattgaa tcaggcagga cggcacccgt tcaggggatt
     4561 gtagacggaa gcgattccaa caccgcatct atagctattt cttatgctca ggcagttaca
     4621 gcggcgtact cgcaggatat cgctctccgc actttccaac gtatgggaga aagaacagcg
     4681 gatcttcctg ttcaggtaga ggcacgcgtc tggttcaacg atgaccgtga atctaaaaac
     4741 tatattatcc cgggactcat cgccgtcatt atgatggtta ttgccgcgct gctcacttct
     4801 cttacgattg cacgggaatg ggaacaaggc accatggaac agttgatctc aacgccggta
     4861 aaagctccgg agctggtcat cggaaaactt ttcccctatt ttgttatcgg catggtcgat
     4921 gtgctgctct ccgtgcttat gggcgaatat ctttttcacg tgccgctgca tggaagcata
     4981 cctctccttt ttgccatggc ggctgttttc ctgatgggcg ccctttcttt aggcatactg
     5041 atcagtatcg taaccaaaag ccaactggtc gccagtcaac ttgccatggt actgactttt
     5101 ttgcctgcct ttcttctctc cgggttcatg tatgcgattt cgaatatgcc ccgtgccgtc
     5161 cagctgctga cctatctgat cccggcgcga tactttgtcg ctctgctgaa ggggatctac
     5221 ctgaaaggaa cggggcttgc cgtcctttac caggagatca tactcctttc tgttttcggg
     5281 ttgctgatgt ttttactggc tatcgcaaaa tttaaaaaga aactggtgta atccatggtc
     5341 gctcgcgtca cacatatgct cattaaagag tttattcaga tcttccgtga tcctcggctg
     5401 aaaggcatta ttttccttat gccgatgatt cagctgctgg tcttcggcta tgccgttacc
     5461 accgatgtca atcaggttgc tacagcggta tatgatcttg acaacagcag tgccagccgc
     5521 gaactggtcg ccgctttcag cgattccggg tattttaacg ttgttgagca catcagccat
     5581 ccggatcgaa ttcatcaagt tctggatcat ggcgatattc aggcggtact gcagatcaat
     5641 tcaggctttt ccggaaacat tgagtcgggg cgaagcacca gggttcagct gattgttgac
     5701 ggcacggact ccaacacagc cggaattgtt atgaattaca gctcaaagat caccggtgcc
     5761 ctgtcccggg aaaaacttgc gaaccgctta agccgaatga aagggcgaat gccggagata
     5821 ccggaggtca cgctggagcc gcgtgcctgg ttcaatgaga atcttgaaag ccggaacttt
     5881 tatattcccg gggtgatcgc cattattgcc atgctgataa cacttatgct caccagcatg
     5941 gctgtggtgc gggagaaaga agtcggcacc atggagcagc tgatggtatc ccccatccgc
     6001 cctgtggaac tggtactcgg aaaaacaatt ccttttgctt ttatcgggtt tgccgatgtt
     6061 cttcttatta ccgttctggg cgttttctgg tttaatgtgc caatccgggg caatctgttt
     6121 cttttattct tttccacagg cctttatctc atgaccacac tgggaatcgg ccttctgatt
     6181 tcaacagtga gccgaaccca gcagcaggca atgatgagca cctttttctt ttatttccct
     6241 gctgttctgc tgtcaggttt catgtttccc attgcaaata tgccgacagt tattcagtgg
     6301 cttacactcc tgaatccgct ccggcacttt ctcattatcg cccgcggcat tttccttaaa
     6361 ggtgtcggcc tgcctattct ctggcctcaa ctggtctttt tgctgattat gggcagcgct
     6421 gtcctctggc ttgccacccg gcgttttcac aaaactcttg agtaaattat caaggagact
     6481 ccttatgact caccgttctt tttcactgcg tatttccgca atgctcttac tggtcttgag
     6541 ctcgtcctgc agcaccgtga caagcgattc catcgcaagg attgaaaatt attatccgaa
     6601 acccgccgaa gaaaatatgc tgccggtaaa gaacggaccc gctccagagg agagcgtttc
     6661 gcctgtaccc gaacaacggg ctcttactct ggaacactgt gtcgcgatcg cattggacag
     6721 aaatcccctg cggaaagccg cagaagaagg aatcgctgtt gcccgcgcca atacaaatgc
     6781 cgtggcatct cagtattacc cttctttaac ctggtcggct tcctatcgcc ggtgggaaag
     6841 ccacgctttt ctgccaagtg atctggggct tccgggaaaa aagccttcat ccatcggccc
     6901 ggtgagtgac tggtcaacgg gtctgaaagc gcagtacctt atttttgaca gcggcaaacg
     6961 ggcggcagaa ctgcgcgcgg caaaatccgc ggtggcggcc gtttccgaag aagcagagaa
     7021 attacagcag gatatcgctc tctctgtaca tcacgccttc tatcagctct gcgccgcccg
     7081 ggaaggatat gccaccgctg aacagaacaa aaaaagagct gaagcgcaca ctgcccttgc
     7141 tgcccgtttg tgggaatcag gtgctgttcc caaagccgat gtcattcggg cgcaagtgga
     7201 tctttccgaa agcgctttga aacttgtaca gtttgagaca gccgtcaaag aagcccgggt
     7261 aaatctgaac cgtgccttgg gtcttcctct tgagatggaa tttgcgctgc agaccgacat
     7321 gccggttctg acggatccgg acacaataga tatgaacacg gcagtgggac aggctctgca
     7381 tcaacgcccg gaacttaaag cagcgctcca tctgattgac cagcagcagc acctcctttc
     7441 cggcgctgaa agtgccttcg gccctacggt tgccgccagt ctcggctacg gctacagaga
     7501 tgacagtttc cctgtgaaag ataaagactg gtctgccggt atcggcattg aattgcctat
     7561 ttttgatggt ttccggagaa aaaataatgt tgcaaaagaa aaacatgcct ttgcccgcca
     7621 ggaagccctg ttacgtcaga aggtgctgga gatatccgaa gaaatctggg tatccaaact
     7681 gcattgggcg gaatcttttg ctgcgattgc gctttctgaa gccatcgttc acgatgcgga
     7741 agaaagtatg cgcatgatcc gggagcgtta tgaagcagga gcagcaacgg caacagacct
     7801 tctcgacggc caatcaaaac ttgtcggggc acaattcggg cttgtagaag cacactggcg
     7861 gtttttcgca gcaaaagcac gttttgacca cgcttgcggc aatctcgtcg ccgcatcgga
     7921 ttaagtgtat ttcttcttcc cgggtgtgta taatgctttt cgaccctgtg aaggtataag
     7981 gagagcatat gatgcatcta tggaagaaga gtactttatt cagtttgggc cttattttgc
     8041 tggctccttt tgctgtttct tacggagatg ccagcacgac cgggacgccg cgtctcatgg
     8101 aagggccgat ggtaggcgct gtcagttcga cggaaatcag tgtgtgggtc cgggcaagcg
     8161 gagaatttcc tctggcgctg gaatatgatc agaaggcgga ttttaaatct tcgaaacgaa
     8221 cagaaaaagt cgttccttcc atagaaaaag acgatcgtac ggcggtgctg cgcatgagcg
     8281 gacttgaggc ggacacgctc tatcattacc ggatactgat caacgataag cccgccccct
     8341 acatcggcgc attttgtgcc agaacagctc cggctggacc cgcccgattc accgtatcct
     8401 tcggctcctg cccccgctac cagcaggacc ctgtccagaa catctggaag gggctcgaaa
     8461 aagtagcccc tgatctcttt ttctggctgg gtgacaacat ttacggcgac actttcgatc
     8521 ccgatattct ggcggaatgt taccggtacc agcgtgccgt ccccaactcc agacattttc
     8581 ttgccaccac tccgcagctc gccatttggg atgaccatga tttttcactg aacaaccata
     8641 acagaaccaa cccgattaaa gaagcgtctt tgggtgtttt caaacgctac tgggcaaacc
     8701 cttcctacgg gctgcctgaa acaccgggcg tgttctttaa atatcactac ggcggtgtgg
     8761 actttttctt tctggacgta cgctatcaca gggatcccaa tacggaacct gactctccgg
     8821 aaaaaacgca tctcggcaaa gaacagcttg cctggctgaa agaagaactc ctcgccagcc
     8881 gcacaccatt taaagtactg gtctccggtg gcggtttctc caatgaaaaa ggaccgaaag
     8941 gcgattcctg ggcttccttt ctctatgaac ggaataatct gtttgatttc atccgggaca
     9001 gagaaatttc cggagtactg tgcttttctg gcgacaccca tgttggcgag ttcaatgcta
     9061 ttccctggag cgaaaaaggc ggctatgatt tttacgatct ggacagttcg ccgctggctc
     9121 aaaaagcaag cgacaactgg aaacaacgaa ctcctgaaat ccgtctgagg atgccgtatc
     9181 aggcgaataa tgcaggactg gttgaatttg atatgacagc cgagccgccg acggttcgtt
     9241 taaacctgat gacggaaacc ggagagccgg tgtgggatcc tgtggtgctt acttccgatg
     9301 atctgaaaaa tgggactgcc actgctgaag ccaacatcaa actcgattga gaaagaagcc
     9361 gtttaacttc ataacacatt cattcaacga ggaaagagat ttgtatgtct gagaatatca
     9421 tggaaccatc gtcggaacct gccaacgctg cagaggaatc cccggcagcg gtacagcaga
     9481 agcaggagca gaaacctgta accgttaaac cggagcgcat gatgtccatt gacgcattgc
     9541 gcggctttga catgttctgg attgttggcg gtcaggggct tgtggtcgcc tttttgctcc
     9601 tcttctccgc ttcaggagag cttccggcgt ggctcgatta tcaattcagc catgtggact
     9661 gggtcggctt ttctgcctgg gatctgatca tgccgctttt cctctttatt tccggggcat
     9721 ccatcccctt tgccatgcac ctgaaagaag gggaaacgcg ggattacaaa gccttttatg
     9781 tacggctggc acggcgtatc gcccttttgt ggatcttcgg catgatcgcc caggggcggc
     9841 tgctcaccct tgactggacg cagttccgct tcttttccaa tacgcttcag gcaatcgccg
     9901 tgggctatct gatcgcagtc ctcgcccatc tttatctgcc gacgaaaggt cagattattc
     9961 tttttatgga cgtactgatt atttactggg gacttatgat gtttgtgcct gtgcccggcg
    10021 ccgacggctg gacgctggaa ccgcgtctga atcttgcgct ctggctggat gacaccatac
    10081 tcgggcgttt ccgtgacggc acgacctatg cctggattct gccgggtctc ggttttgctg
    10141 gctccgtgat gctgggtgtt ttctcaggca tgattctgcg attgaaagaa gcgcccatga
    10201 aacgcttcgc ttatcttctg ggactgggtg ttgcctgtct cgttggcggc tggctgtgga
    10261 gcttccactt ccccatcatc aagcacatct ggacaagttc catggtgttg tgggcaggcg
    10321 gatggagctg cctgcttctt gcgctcttct acctggtgat tgatatctgg aaatttcagc
    10381 gctgggccta ccccttcatg atcatcggcg ccaatgccat tgcggcctat atggcagagc
    10441 atctgatccc cttcaaacag ctggcattgc tgattcccgg tcttgcatgc gagtgccctt
    10501 atgccgcttt ttttgcctct ttcatcgcct ttatgctgct ctggatcccg ctgtggatca
    10561 tgtaccgata taaaattttc ctgcgagtgt agccggagtt tcaccagcct cttatagtga
    10621 aaaaacaaaa ggctccatta ataaggaagc agaggacatg acttcttttc tggaaggtct
    10681 gcgggcatgg cgtgcattga atcacagaaa agaacagcct gaagatccgg aaacacatgc
    10741 gggagcgctt gctgaaaatt tcctgaaaag acttatagat tccaacttga agttcaataa
    10801 cggctacgct ttcccgggca aaagaattcc gtcccatatc tatggtaacc gttatgaagt
    10861 ggatctcatt gttcttacgc caaaatatat tcatgtgatt gaagtaaaaa actggtccgg
    10921 gacgcttcag ctgcaagacg gtcaatgggt gcagactcgg agaaatggcc aggaaataca
    10981 gcacgcttcc ctctccagcc tcaatcagga gaagaaagag gcactcatag cctatctgca
    11041 caaccggagc atacccgtca atccttccca ggtcacccag aagatcattt tcatgaataa
    11101 caacctgaga attctttcgc cggagatccg gaaagaccct catattattc aggcctatga
    11161 actcaagcac tatctggact ctcaatggaa aagcgcctca gatgaaaaga ttgtgcagtc
    11221 aatcatcgag gtgctcctcg ccaaggaaaa gcaggagaaa gcattttcaa gaagtgtaca
    11281 gggaaaatat ttctacgcaa cacttttttt gctgcggtct cttgaaacct gggataggct
    11341 tcgtcttcat ggaggaaaga cgctggtagg cgatgggctg cagctgatca cgcacagaag
    11401 atccttcgat ctgaaaaaat tgccgagcgg cacaaaatat cctgtaagct ggtcgcagcc
    11461 ctctcccctg ctctatcttc tgtgcctcct cggcaaaagc accgtgggct ctataaacct
    11521 gcctaaccgg aaaattgcga tttcacctca tgatatgatt aagtttcatc aggcaatggc
    11581 gacaaagccc agcgtcatcc ctcttggcga agtggatctt tttattcgag gctgaatttc
    11641 tctgaccgcg cccttactgg ctttttaacc agtgcacaaa acgggcaatc ccttcgtcca
    11701 tcgtaaagcg tggattataa tccagcagtg cacgggcatg ggaaatgtct gcataggtaa
    11761 tttccacatc accgggctgc agcgatttct cttcaatttt cgctttacac cctgtctgct
    11821 tttccagcag cgcgatcagt tcgctgagcc ctgttgtatg ctgttccccc agattaaata
    11881 tttcatagga aaagggtctg tccacgcatc ccagaacgcc ctgaataata tcatcaatat
    11941 aggtataatc ccgccgtgta tcgccttttc cgaacatggg aatgggtttg ccttccagca
    12001 gcaggcgcgc aaatttgtgg atcgccatat ccgggcgctg gcgcggccca tataccgtaa
    12061 aaaaacgcag catggcgact ttgacaccgt acaggtgact gtaaacatgc gcctgcagtt
    12121 cgttcattct tttggtcgcc gcatagggac tgatcgggcg catcacggga tcggtttctt
    12181 taaaaggcac ctctttgctc ccgccataaa cagaggaact ggaagcaaac accagatggg
    12241 agggcgtata tttccggcac atgtccagca tatgctgccc tccgataaca ttgacacgat
    12301 gataaagatt ggggtcttcc agtgaaggtc tcacgcctgc ccgcgcagca agatgaacga
    12361 ccacatcagg ccgttcctga ttaaaaatct gatccagcgc ttcttcatca caaatatctt
    12421 tttcgtaaag acggaagttc ttctgtgaaa gaaggtctct gatattattc ctcttcaccg
    12481 ccggactgta ataatcattg aaattatcca gaccgatcac ccgatctccc cgggcacaga
    12541 gcgcatcaca gaggtgggat cctataaacc cggcggcacc ggtcacaagt acacttttca
    12601 tgagatgcag tccttattcg gagatacgat atgtaaacag atgcagcggc tcatactgtc
    12661 cccggtgata taaagcatca cgctgcgtac cggcactgac aaagctcagc gcttccaggg
    12721 ctgccataag ttcctgctcc cgggcggatg cgtaggcaat caggtgccgg attcccggca
    12781 cggaggccat ctggttgaga agcgtgccga gaacggtatg cagagcgctt ttgccataag
    12841 acgcaggatc atgcatgtaa agccagatat gagcaaggtg atgtatcgga tccggcgcta
    12901 ttttcagaag accgcagggc ttcccgctct tatccgtcag cacatattcc tgcgcaccgc
    12961 tccgggggtg cgtggcatcc atctccttat cataaagggt tgtcgtctgc acgtttgttt
    13021 ctgcgcacaa aaaaaatgcg gcatcgttcc aattttccca gcgcacccgc cagtccgcat
    13081 ctgattcctg cagcggacgg gcaaggatct gttctgtaaa cagtgccttc aaggctgccc
    13141 cccactttat tttgggggcc gatgttttgt gcgtcgacag cggcggatca aaggacaggg
    13201 gcaatgatgc cagaggggta aagagagcga cggcatgctg agtccattga cgctgcgccg
    13261 cgcaccactg ctcttccggg cggaaagggt cgcctattct tgcggcatgc acagcgctgt
    13321 atccgccgcc gcacagaccc tgcgcccagc accgaacaca ttcagcacag gtcagcgtgc
    13381 tcttctgctg aaattgttca taaatggatt cattcataca atgcgccgca agatcaccca
    13441 gagcaaagct gcgcgctgtc ataaaatcaa cagacggata aatctgcctg tcctccccta
    13501 tcgccagtgc ggagaaaccg gagggatccg ttctgtagca cggctctccg tcacggatcg
    13561 ccttaaacag atcgctcaca gggtaaaaat aaagattctc ctgccggagc agcattgccg
    13621 aatagtcctg cgccgccttt atcagttggg cagcaacctc atccggatca tgtgccggct
    13681 gatgcatcca taaaaacgga agatccagac ggatattttt aaagcccgct tccaccgcct
    13741 gtttaaaggc atcactgaaa gaagcagtct gcggttgaat gatcagctgc agagggcacg
    13801 caccttccaa cgcttccagc ctttccagaa acacccgact tttaagggtg ttctgcgccc
    13861 agagcacttc gcgcacactt ttgatcgcac caacacctgc tatgggcaga tgaacccgaa
    13921 aggagcaggc aggatgctgc aggtcttttg taacaatagg gacatccagt tgaaaagaga
    13981 gcttcttacc ggcagaaagc acggcatctg cggcatactg cagtgtattc agcgcctctt
    14041 catccaaccc tttcccacct ccggtgcagc gcagaataat ggttaaatcc tgctgtgtgc
    14101 ccgaacgtcc tagaagaaaa ggcagacagt cccgaaccag cgccgctgtt ccatccgtgc
    14161 tggagcaaag ggtcagacag gtcacttccg ccggctgatg cacgattttc agccattcct
    14221 catcattccc cggaaccaga atagatcggg tcactcgcag ataatccagc tcgccgatca
    14281 cctcctgcac ttctgcttcg gggaaccggt ctttcagcag tgtcataatt cggcgggcag
    14341 attcctgcgg atacagctcc agcacagcac cggtaatttc gtcgcattca aaacagaaac
    14401 aattgtgcgt atccagcaca aagtgccgac ccccaatctg aaatctgtgc aagcgctgta
    14461 ctgtcatcag tagcttccga gtgttccttt ggaacggctg taatcttcca tcagtttaaa
    14521 gagtgtcaat cctacaaaag tccagaagag gcagtttccg ataagccagt aaaaactgct
    14581 atgggcaaag acagaagaaa agaccatttc aggcgcggca ccggcggtag aaatatcacg
    14641 catctgccct ttaatcagca catacttgat gcatatggtg gcatccgtaa tggggaggac
    14701 atgaagcaga aaagtgaagg gccagggatt gccgagcagt tcctctttgg caaaaatggc
    14761 gagtgcaaaa agtcccatac gcaccagaat ggcgacctgt cccacctgct tgaaaacaag
    14821 caccagaccg cccaccataa agccaaaacc gaccaggtta aaaaaggtaa gcgtcagcaa
    14881 tatcaggaca ggtatggtgt cccaatgcag catgcctccc acactccagg taacagcata
    14941 gaccatgatg cccgaggata aaatcgttgc aacggagctg accacggtct gtgccatgaa
    15001 attcacagcc agcccgtgag gactcatgca gagctgttcc agaacgcctg ttgccgccat
    15061 gccctgaagg ccctgggtga acatgcccac gacgccgaag gcaaacatgc cgacaataaa
    15121 gcccagaata aaattggttg ccgatgaggg atcatcaaac tgctgcaccc gttcctccag
    15181 cccctcacgg ctgtagataa agccggcgac aagcaccatg agcatgccgt atcccaaaac
    15241 caacccggta atggtacgaa accagtagcg tcgcataata atgaaagagc gtaccagctc
    15301 cgctttcata actaagagga aagacatgta tttcctttct tcagtacatc tgcatcagga
    15361 tcatctgagg taagccgcag gaaaatactc tccaaattct gcttcctctg ggaaacagat
    15421 cgcagaaggc atttctgctc ttcaaagatg cggataattt tatacaaagc ctcggagcgc
    15481 agatcctgct gttcgcccac caactgaaac acaagttccg gttcctcgct gccttcgtat
    15541 tcgcttttga taataccggg aatgccggaa aacagatcca gcggcacccg cctgtccagc
    15601 cggaacgcaa acacctgctc ggaaaaaact tcaagcaggg aatccgtcgg ccggcaggca
    15661 atcagttttc cgtgattgat tatggcaatg cggtcgcaca gctcctgtgc cacatgcata
    15721 tcatgggtgg tcaccagcac actgcgcccc tgctcccggg taatttcccg aaccttgtct
    15781 ttgatcgccc ggctgctctg cacgtccagc cctgtcgtcg gctcatcaag aagcagtacc
    15841 agcggatcgg caatcatggc aatacagatg gcaagtttct gtttctgccc ccgtgacaga
    15901 cgccgcacct gtacattttt tttatcctga agcccgataa aatcgagaag atcatgagca
    15961 cgctctttca gaacagagcc gcgcacatcc ttcagcattc cgtagtacac gagattttca
    16021 aaaggcgtca gcggccagag actcgtccgg gtgccttcca gtacgacccc gacatgccgc
    16081 aaaatctggc gccgcttttt ttcaacattc agccctttta caaacgcctg cccggcatca
    16141 gggcgcacca gaccggaaat cattttgact gccgtagtct tccctgcgcc attgggcccg
    16201 agcaagccga atatttcgcc cggtgcgatg gaaaaagaaa tattgtcggc agcacatacc
    16261 acttttttct taaaaagacg ccgcttacgg gtttcctcat ccaccggcag tacgtgagag
    16321 gttgaaaact ctttgcatac ccctctcaat tccacgaggg ctgtatctgt catgacgcct
    16381 ccagaacaag gttgttttgg ctgttttcaa ttgttttaac agcgtcttcc ggagcaattc
    16441 catcctgaac cagggaaaca tagtccagat aagtgctttc cagatttctt ataaaatgaa
    16501 cgaatacaac gatggaaatg ctccatgtaa aggcatagag cgctccgata acaagggaaa
    16561 ataaaaagaa ctcgatggcg gacacccagc cgctgcggaa aaaatgcccg tgcatcacac
    16621 tgaaaaaaac agccgtcagt ataacagcca gccatgaggc gggcaataat tgaatcagga
    16681 tcgattggcc ggcgacacgg tacacaagtt cctccacaag acaaagtccc agcagtcgta
    16741 cacacagcag aggcgcacgg gcaacgaatc gaaatatgtc ccgaaaggat aaaaaaagac
    16801 ggaatacgtc accgggctga cgatgggtaa tcagcagcgc aaaaaagaaa aggacgtgtc
    16861 ctgtcacaat cccgaggctg atatcaaaag gcgaccatat ataacgggta agcagcgcct
    16921 ggttccacgc ccacacggcg gcaacagaaa gcacgatacc ctgcagtacg gtgtccggaa
    16981 aaaaaaccag ggggattttc cggacatgtt taaatccgga aatgcccgta tgaaccaacc
    17041 acagcgatag aaacagaatg ttcactttgt gtccccgctg tcgtctgcca tcgctttgat
    17101 catggcatcc aacgaaatac tgcctgacac agcacccagt ttttcacgca gggcataaaa
    17161 ctcatgacgc agcaaaccat agccataggc atcatgaagg gtcttgttcc ggtaaatatg
    17221 ctctctcagc accaattccc tttttgcacc ggatttttcc atcatgcgcc aggagcgttc
    17281 gttaaacgca tagatcagcg aactgaccct gtgcagatta agctcatcaa agacatattc
    17341 gagggcgcga aaaatagaaa cggcaggcag aatactggaa cggtatgaac gtgccccgat
    17401 gtatatatcc aggctgcagg agcggtttct ccaggagata ttctgcagac cgatcatacc
    17461 cagcagccct tcctctttac tgtctatgag caggtgaatg gcggaaggcg gagcagcggg
    17521 cggtgtccga ccaagcatca gaataatctt ttcccggatc tgcctttggg actgcgcttc
    17581 atctccatag agaaaatatt ggaattccgg gtcttccatc caggccagaa tgctgtcaag
    17641 atcttcccgt tccgcccggc gcagaaaact gtgcagccct ttcttcatga cttctctccc
    17701 cctttatcct gggatgcctg tttgtagaag gtttcagcag taagttccag cgtttcgata
    17761 cccgtccagg tgccgctgat gcacatgata tcccgctgaa tgccgcagga ggaaaagcct
    17821 ttgcgcagga gaaactggcg ccatgccttt tcactttcca gacagctcac gaggacgcgc
    17881 cccagatcgg tttgaaaaaa agcacgatca agaagataac caagtgcctc atcggcctga
    17941 aggctgtcat accctgcttc atcggcaaaa acaagaaaca gttccgcaaa acgggcatcc
    18001 gggttcaaac cgcgcaaccc tgaccaggca cgcaaaatcc ctgtaccatc ttctatggag
    18061 aacagcatcg cccgtgccgc ttcgctcttc cttataatct ccagaatttc acttcgcagc
    18121 ggaagcatcg gctcccggcg tccgtccaga agcgccgcac gcggtattcc atcgtaataa
    18181 aggtcccgga tgaaatcagc gtcatcgggt tcacaaaaac gaacagtcgt gtgagggctt
    18241 aatttcataa cctgcacttc cggatcagga aacattgaag atagtataac aataaccggg
    18301 gcacaaaaat gagggcgtgc agttcatacg ccgcaggaaa aacaagtctt tattttccta
    18361 tacaaaattc attaaagatc agatccagca cttcttcagc agtggtctca cctgtgatac
    18421 cgcccagcgc atgaaccgct tcacgcagat caagtgccag aatctccgga gaggcagacg
    18481 gatgttcaag aacccgctgc acacaagcca gcgcacgctg catactctcc ttctgatggc
    18541 tcctgaaaag agccggtgca tccagtgcgg catccagatc accggcaagc aatgttacca
    18601 gtgtcttttc caaaccatcg ataccctccc gcgtcacggc ggaaacagga catacggctg
    18661 caaaaggaat atcgggaaaa tttctttcgt gaagaggcgc aagatcgatt ttgttcagca
    18721 ccaggacaac gggcacaccg agctgaagca tttccgccgc cagtcgcttt tcttcctcat
    18781 ccgcccctac ggaggcatcc gccacaaaaa gaagcagcgc cgcccggcgt gccgcctgac
    18841 gggagcgcgc aattccctgc gactcaatat gatgctgtgt gtcccgaaga cccgctgtat
    18901 cgcacaggcg gatgggaatt ccgccaagcg tgagtacttc ctgtatatga tcgcgggttg
    18961 ttccgggttc aggagagaca atggcacggg cgtcacggag cagcacattg aacagactgg
    19021 atttccccac attcggctga cctacaatcg ccagagacac gccttcctgc aggagcaaac
    19081 cggtttccgc acttgccagc agttcccgca tttccctgct cacctgctcc agatttgtaa
    19141 aaaaaacagg cgtgatgagt tcgggcaaat cttcctcagg aaaatcgacc gaggcttccg
    19201 catgagccag gcagtgcttc aactgctgct ggagcgcctg aagccgctca gacagacgtc
    19261 cgctggcagc attttccgcc agacgcagcg cctccctgct tttagcctgt atcagtgcca
    19321 taacagcttc cgcccgggtt aaatcaattc tgccattcac aaaagcgcgg cgcgtaaact
    19381 cgccgggcgc ggcgagacgt gcaccctgcg ccaacgcagt gtcaagaatt gcctgaagcg
    19441 aagcgcgtcc gccgtgcccg ttgatttcca ccacatcttc agccgtgtag gaatgcgggg
    19501 catgcataac atgcagcaga acctcatcaa cagggaagcc ggcggcgtca attatatgac
    19561 cgtggaatac gcgctgtcct cttgcccgga tgtcttttcc ccgggaagac cggaagatcc
    19621 gggaagcgat ccggagtgac tctgtgccgc tcaaacgaac aatccccacg ccgccttcgc
    19681 cgggcggtgt ggagattgcc gcaatcgtat cctctgtaaa taagaaactc atctttcgcc
    19741 gggtgatgcc gcattctgct gtttccgatg cggatgcgaa cgctggggac ggggggcgcc
    19801 gccccgacgg atatttaccg gattaataat aatgtttctg taaagagaat caccctgact
    19861 gtaggttctg acatcagaat ccttttccag cgccagatgg acaatacggc gttcctgagg
    19921 gctgagcggt tttaacttga cattacgccc tgtctcttta gcgcgcttcg ccatcttttt
    19981 tgccatatcc accagagcag cacggcggcg gctcacatac tcacccacat ccaccataag
    20041 ccgttccgta ttttcattgt caccactggg aagcatacgg tttaccagat actggagggc
    20101 atttaacgtg atcccgtgct tgccgatcag gatggcactg tctgtttcac ttttcatcga
    20161 cagacagggg ctgtcgcttt catcacggat aaatcgaaca tcggcagtca gccccatgcg
    20221 gtccacaatc tctttcagca ttgccgccat ctcattgccc tgcgcatcac tgatcggctc
    20281 gattgcctct tcttcatggg caccttcagg aacaggctca acagtgagaa ccgcttgcag
    20341 gtcatcatta ttggcaaaca tttccgccag ccgtgtcgct tcagttcctt cctggattaa
    20401 agaggcggct tttttcttct cggcaaaaga accctgcctt tcgcccgaaa ggctcacagc
    20461 ggcttcctga tctttttcag cacggtgttt ggatctggac tcttcggaag aggcatgtcg
    20521 tttctgcttg ttcctgcggt tttgatcgcc tgaagcgcga ttttgcccgt ttcgcctgtt
    20581 ctgctgatcc cgctggttct ggggttgtga ctgccctttc gctctttttg actgccgatc
    20641 ctgtgctcca tcccgtggcg cttgccgctc ctgttcattg cgtgcaccgc cctgggatgc
    20701 tttttcagcc gtatttctgt cccgtctgct gtcgtgtcgc ctttcctgac ggccctggct
    20761 tttgttactt gagatagcag ccatgtccac agtcagccgg acacgcacgg gccgtttgcc
    20821 catgcctaaa aaacctttgc tgccttcatc gataatgtct atgcgttcca cttcatccat
    20881 ggcaacgcct aattctgtca gtccgttttg aatggcttct tctctggttt gcccggagct
    20941 ttcaattatc ttcattaaac tttttctcct gcatcgcccg gaggcggtag ttggttcggc
    21001 gcagaacact gtccgctgcc tgccctcagc tgcgtccgcc tgagtctttt cggcgtcggg
    21061 accgctgttt ctgtttgcgg atctctttgt tcatttcacg ctgtttaagg cgtgccgcat
    21121 cataaaaatg acgtggccgc gagggcttct ttgtctttac cggttcggca tctgctttga
    21181 tgccccgtac caaatagttc tgggcgatgc ccagaagggt gctgacgaag atgtaaagat
    21241 taagccccga tgccatgtta taggtaataa acccgaaaaa tacaggcata aaggtcatca
    21301 tcatcttctg ctgctgattc tgcaccgggc cggacatggg catcattttc tggctgatta
    21361 ccatggacac cgccatgagt atgggaagca ggttcaggct gtcaagaggc acgctggaaa
    21421 aaggcatggg gatactgaag ggcagtgtca gcatccggtc tgcctcgctc aagtcctgaa
    21481 tccagaaaat gaagggggcg cggcgcaatt cgaaggcgct ccagagcatc cggtacaagg
    21541 caataaacac gggcatctgc agcagcatgg gcagacagcc acccaacggg ctgaccccac
    21601 gctctttata aagcgccatc attcttttct gcagttcctg ctggtctgaa ccgcactccg
    21661 ctttcaactt ctccatttcc ggtgccagct gggacatgcg cttcatgctg atcatgcttt
    21721 tccaggtcag cgggaacata accatacgca ccagaatggt caggaaaata atggcaagtc
    21781 catagttggc aatgatatgg tcatggaacc agatcagaat tgcaagcagc aattttgcaa
    21841 aacgatccat aagagagaag gaagtgaaaa aggagaaaac ggaatccaat cccggccacg
    21901 cctgcaccag atactgggtc gctctgggtc cgacgtaaac ggaataattg aattctgccg
    21961 cttcatccgg agccacttcc tttctgggcg cacccagtcc cagacggaag tagtctttgt
    22021 ttccgtaata ccagctgtcg gcgaattcaa attccggctt gatcgccacg gtaaaatagg
    22081 cgctgctgat ggcggcccaa tcggtgtcga gaagacgctc gttaaaccac ccgccgtcat
    22141 tgacaggctt catctttgct gtggcatgga aggtgttttt accctcctta tgaaacacaa
    22201 tattctgctg ggagcctttt gttaaatcgc cgctggtaac attcgggccc cagtagagag
    22261 aaagtgcggg ctccttgcga tccagaccca ggagccgggc cgcttcgccc caattttcat
    22321 agcgtatggc tgtctcaagc acataactgt cgggctgcag agaaaagcgt ttttccagaa
    22381 gggcatagcc tggaacctcg agacgaaaaa ccaacactcc cggttcggat gattcaacgc
    22441 tttcccagcg gcaataattc aattgatcgc cgagtgcccg atcgctgaaa agtatggaca
    22501 agggatacac gctttccgca tcatcggcag gctctgcatc gggcaccagt tggatggaat
    22561 cctgtccgtt gctgccaagc agcacggttg ccgctttgag ccgggcgccc acccgggtga
    22621 aagtcagctc caactgattg ttgctgagaa agagttcgtc atcggaaggg ctttcaggag
    22681 gtgaagcaac aggcggaatc tccggatatc cttccggaat agtgcgcgca ttttcttccg
    22741 ctacagtgcg gtctgaacgg tcaaaaagac cggcacctgc ccgttcttcc tctgcggtgg
    22801 attccgtgcc gtcttcctgc cggggcggcg caggcggtgg cgcagcgggc atgaaaaaat
    22861 atgtccagac gaagaccaga acggtcatca atacaatggc aagcagctga tttcgctgca
    22921 actgcctgtc tctatcatct cctaacaata cactggtact cctgccccgc agccgggggc
    22981 tgtcttcgga gaaatcaatt tactggggat cacgggattg aggaaatgct ttacttgacg
    23041 gcggcaccgg atcatagcct cccttgcata aaggctggca ccgcagcaac cgccacaggg
    23101 tcagaaagcc cccttttaaa gtgccatgca cccttaaggc atcaatggca tattgggagc
    23161 aggacgggac gaaacggcaa tgagagccta aaaggggcga gatccatttc tggtatccgc
    23221 ggatcatcag gattaaaatg ttagacatgg cgcaaaaatc gcttcagcgt ggcatttaat
    23281 tcagaggcac aggctacgct gctctggcac gcaccggaag cccgcgctat gatgaccagc
    23341 tgcatccccg gctgcaagcg aaaataattc agccggaaaa actcgcgcaa acgccgcttt
    23401 accctgttgc gcaccacggc atttcccact ttccgggaaa caaccagacc gagccgggcc
    23461 tcccgggacg gatcgagcag gacgtaacag atataagaac gcccccacaa cttttcgccc
    23521 ttactgaaga caagatcgta ttcatgccgg gcagtcagcc gctgtgttcg cagaaatcgg
    23581 aggcgttcac acacgcggaa atctctaccg cggaattgaa tcggaaacgg tcaggcgata
    23641 gcgtcctttg cgccgcctgc tagcaagcac ctgacgtccg ccgggcgtcg ctctacgggc
    23701 acgaaagccg tgcgtgcgta tgcgcttggt atttgacggc tggaatgtcc ttttcatagc
    23761 aaactcctgc actctatcga gctaaaatta acatatgcgt atgggaaaaa caggcatgca
    23821 tttcgctgcc tgaaaagcca cctggaaact ccgcttcctg tcctgtttgc ttcttgcgaa
    23881 acaggcggaa tacatcaccc ccgtcgccat ggactatagt gtaccttttt taaagagaaa
    23941 aagtcaagaa atgacagagt ccctgtcgga gaagctccat tattcccgga tctttcctgt
    24001 ttaaaaaaga ctcagccaaa gattccggcg atacacgccg tcataaaagc agccagc
//
LOCUS       DAOPWB010000038        23628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2138_length_23628_cov_5.995800, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000038 DAOPWB010000000
VERSION     DAOPWB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 23628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23628
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2138_length_23628_cov_5.995800"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PKN92_05315"
     CDS             complement(<1..1204)
                     /locus_tag="PKN92_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ48244.1"
                     /translation="MLNLAHFLNETAETYPDKTAVILDDLRFTWAEVAALARKVANIL
                     AAKGIGKGDTVALMIPNTPHFPIIYYGILHTGAAVVPVNVLYQYHEIEHYLSDSGAKA
                     FFAFKLFEEEARKAFLAAESCKDLIIVSTPDDLETPTVGENFNTLLATADSSFETVPT
                     MPDDMAVVLYTSGTTGAPKGAVLTHFNMFFNAYYACHHIVKAAPDDVTLVALPLFHSF
                     GQTCQMNASVIGGATMTMLPRFETEKAMEVISRDKVTVIALVPTMYFFILNPENWQDY
                     DFSSVRMAISGGAALPEEVHHRFRERYGIVILEGYGLSETSPVASFTLPGEEVRVGSI
                     GKPIWGVQMRILRDDGQLGKTGEIGEILIRGHNVMKQYLNRPQATRDAIVDGWFRTGD
                     VGHFDEDGY"
     gene            1552..2856
                     /locus_tag="PKN92_05320"
     CDS             1552..2856
                     /locus_tag="PKN92_05320"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48245.1"
                     /translation="MKSAMNRRHFLKGSLAAGAALTVPQIIPAAAFGQGDTPPPSEQI
                     TIGCIGVGRMGRNNLNDFIKLPDCRIVAVCDTYQENLALAKDIVDTHYQNTDCATYGD
                     FRDLLARADIDAVIIAVQDHWHGIIATAAARAGKDIYCEKPLGISIAESRAILDAVRS
                     HNRVLQTGTWQRSLKPFQHACTLARNGYLGTIHTVEVGSPGPDYYVRHTGDLDGHPVP
                     DGFDWNMWRGPAPEVPYNPGRVAWPDWYLIYDYCVGFITNWGVHHLDIANWGCPRIGR
                     EPFEVVCKETFRSEGFTNNSKKWDARFHYADGLTMIFQDDHQGERGTRFIGDEGWVRV
                     DRDGIWAGPQSLLKVEFKDTDQRLTDSVNHGANLLECIRTRKKPVSDVEEGHIASCLG
                     MVADIAGRTEARLAWDPKTERFLNNDEANALLSRELHNGWTL"
     gene            complement(2872..3720)
                     /locus_tag="PKN92_05325"
     CDS             complement(2872..3720)
                     /locus_tag="PKN92_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ48246.1"
                     /translation="MNPPAYIKEVVQHGFESFQITFWKTIGDTDLKRLAGEVREALAG
                     TDAVISSVAVYGNPLGGRPQDEDTLRSWVELLDTAHLFGCDIVAGFTGRVIDAPIDES
                     IPRLKEVFEPLAQRAADRGLRIAFENCEMGGNWKTGGWNIAHNPAAWELIFDALPYDH
                     IGLEWEPCHQLVQLIDPLPQLRKWAPKVFHLHGKDATVQWDVVRTYGIGGGRPFVHHR
                     TAGFGDTNWTEIIAIMRQAGFKGSIDIEGWHDPVYKDDLEMTGQVHALNYLKQCRTVY
                     VPNPSV"
     gene            complement(4312..5523)
                     /locus_tag="PKN92_05330"
     CDS             complement(4312..5523)
                     /locus_tag="PKN92_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ48247.1"
                     /translation="MKILTMVAAAVLLLAGGSLAQEEIITLTAAEVVGRVLKIHPGIQ
                     EALEAVEEARGHALQEGLWPNPELEAGAEGLHGNRGGDWLAGVSQRIPLFGARALARK
                     AAEAGVAAAGQQALLREKELTALAEMAFYEALAAQESRRIAENNLANAAAMAEMTRQR
                     FEAGDIAEMDWLRAAAEENHQEAELEVLRIREETALAALTVLCALPPGAKPVLEGTLL
                     PEEQAFPDAAVVAERLGSAPAARALELEAEAAAGEVKALKREALPEPEVHVGWRQERA
                     EKQNAVDFSVSFDLPLFDRNQGAIAGARARARRLEAARQAKIQEDCRQALTLLGEGKA
                     ALKEAHLVREKNFPKLEDACRALETALTLGGVSLFEVLAAQQEVAEMRMHLLDLDHRA
                     RHALIELEALF"
     gene            complement(5554..8658)
                     /locus_tag="PKN92_05335"
     CDS             complement(5554..8658)
                     /locus_tag="PKN92_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ48248.1"
                     /translation="MMDKLIRWSVSNRYLVIALSLVFGIFGLYLCFHTPVDVFPDLTA
                     PTVVVLTEAPGYSPSEVENLVTFPLETTLNGAPGIRRVRSASMPGFSIVWSEFDWGEE
                     MNVARQIVAERVQLTIPELPEGIMPPILAPPTSLMGEIQIAALHADNRSMTDLQNYAE
                     IVLARRLLAVPGVAQVTVVGSGDRQYQVALKPERLTARDVTVAEVAEALASNNMNIPA
                     GFISERGTEYLVTGLGRLQNTTDIGKVVVATKRGIPVCVEDVAEVRLGAAPTRGTGSA
                     NGKPAVLLIIQKQPQANTLNLDQVLEDVFADERAKLPEGMALDTNLMKQADFIRVAVR
                     NVSNAIRDGMVIVVLIMAFFLLSGRALLITLTALPLSLIIAVLSVQFFGGSINTMTLG
                     GLAIAIGSLMDDAVIDVENTIRRLRLGRVHLADGNVSPLRIVYEASSEVRSAIVFATV
                     LITVVFLPLYTLENVEGRLLQPLATSYIVAIFASLLVALTLTPALAAVLLPGSGIVQS
                     PREPRVAELVRRAYNVLLGPVLRHPWFVTLPVVAAFVVSVVAFSFMGRSFLPDFNEGS
                     LNVVANTLPGTSLEESNRIGLQIEQQLMEVPEISSVARKTGRGDLDEHAQGIEGSEME
                     ATYLLKERSKEEMLEEVRGRLDRIPGISYGIGGPISHRIDHMLSGARSSIAIKFTGED
                     LYTLRELAEEAQKRIKKIPGAADIAVEPQTDVPEVRVRFDRDLLARHGISFEEAGLTL
                     RAALFGAPVSRIFEGRNAYDLVLRLAEEGDATLASLEKLPLRSASGALVPLNAVASVF
                     RGSGPNVITRDQVQRKIFVTCNPSGRDVTSLVRDIRSEIDPLVAGKEGYAVHYGGQFE
                     SAAAASGRLLASGAVVLVGVFALLFMAFKNGRDALFVMASLPLALIGGVAAVFLMGGV
                     LSVASIIGFISVFGVAARNGIMLVNHIRHLQLEEAVTDFTEAVRRGSVERIVPVLMTA
                     LSTGFALIPLVLAHEAPGNEIQSPMAVVIIGGLLSATFLNMIVVPALYLRLGRPAAKE
                     ETTA"
     gene            complement(8671..10497)
                     /locus_tag="PKN92_05340"
     CDS             complement(8671..10497)
                     /locus_tag="PKN92_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ48249.1"
                     /translation="MISTLMGRTSVRPLFLAALLPLFIAGCSLPKVTEHTHDHGHDHD
                     HGHEHGEEEESSVTLWTDQLEGHVEFGHLDAGTPQEFTLHITRLDSGLPVDAGDAVLQ
                     WKKESDGSTLDVRVPAVRPGNYKTTVTFPAPGHWDLTVKLSLADGEVQLIFPDLDIHD
                     HGHTDGPDAVHDHAHDQEEGHDHDHDHGEDHDHDHDHGDDSDHVHADGTVYASSEDVP
                     GNAILFSKEQQWVLPLVTAQAFRGGLVERYRIPATVQETPHSRAALTAPLSGRLLPPE
                     GGSFPGIGEEVEEGQVLAGVLPTLQGGEALARESNIHSLQGQRTELAVQAAQARGDAE
                     RARALVQQHASALDRAERLQVAKAGSQREVEEQRSALASAQALLREAEQTEAQVRKAL
                     AALPTLESREEEGVAAWIRSPITGRITRLAAGPGTQVDGGQMLIEVVNADEVLIEGQV
                     PEVLAPALSRPLHACYELPAFPGELFEMEAPEGEREPWLQPQVKEESRTVPLIFYTRN
                     QEERLRIGMTLTLLADGKKEEDALQIPHSALVDENGIPTVYVMLTGEHFQKRPIRTGI
                     SDGVMTEVLDGLQEGDRVVVRGAYAVRLAGLAGGGVGSAHVH"
     gene            complement(10567..10998)
                     /locus_tag="PKN92_05345"
     CDS             complement(10567..10998)
                     /locus_tag="PKN92_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48250.1"
                     /translation="MSLIRKIAAVFCPGAVLWGRTVLSLLVAVYSLGFCAHHHPGRCP
                     AASSFVSACCDSGADHGGGSSGMLLSASAHGCRQFHLHGTRAFELPETKMVPGGVSPS
                     FAGELLNLWSLGTRSPAAIPGDSSDSVRSPSFSGRNLPLIS"
     gene            complement(11099..12088)
                     /locus_tag="PKN92_05350"
     CDS             complement(11099..12088)
                     /locus_tag="PKN92_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNZ48251.1"
                     /translation="MLNIAGEGAAFRRDLFYETAMSMAVHPAIAEKDFWVCVLLDFLF
                     NHCKWGKNFAFKGGTSLSKAFGLINRFSEDIDLVLDWQVLGYTRDEPLHERSITQQAL
                     LCQEANLRTAQFLADELAPALRRELPLHAAVPFEIIPWKEDILIRYPKAFSLNAIQPE
                     VRLEIGPMASWLPNASEKIYPYAAEHFPGAFKKTFTVIRTISAERTFWEKATILHQEA
                     HRNSKKQVPPRYSRHYYDLYRMTQSSVAKRALERLDLLEKVVAFKQRFYRSPWASYET
                     ARPGSFCLLPSEYHREELQVDYRKMQAMLFGDIPTFEAIERGLADFEKRINAL"
     gene            complement(12081..12698)
                     /locus_tag="PKN92_05355"
     CDS             complement(12081..12698)
                     /locus_tag="PKN92_05355"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HNZ48252.1"
                     /translation="MKTTGDYILKRMRKAEKGAAFTAKDFLDLAGYEAAKKSLGRLAD
                     TGTLRRILRGVYDFPAYSPVKKGPATPRPDAVAHAIARAHGWTLIPTGETALNLLGLS
                     TQVPAQWQYCSNGPTKCYQWPGGNVLLKHCAGRQTSGLASKTILIVQALKALGRDAVT
                     DEVLGFLRHRLTVTECRQAVSETRHLPIWMQSAIKCLAEKEGIHA"
     gene            12874..14208
                     /locus_tag="PKN92_05360"
     CDS             12874..14208
                     /locus_tag="PKN92_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48253.1"
                     /translation="MDSLTRRSFMKTSAAGAGAAASILLGTSKTSWAGANDRVRIAVM
                     GINGRGRSHLGGFSKLKNVEIAAICDVDSRLFPAVVKDLTKNGKAPRTEQDIRVLLED
                     PDIDAISIATPNHWHSLATIWACQAGKDVYVEKPMTHNIFEGRKVVEAAAKYNRIVQH
                     GTQLRSNPGFQEGINELKNGLIGDVYMARCVCYKWRPDIGKGHPGNPPEGLDWNLWQG
                     PAQEEPFFVNDKGEGIYVHYFWHWVWAYGNGDIGNQGVHQLDAARWGLGVNVPYRVTS
                     MGGMFLWDDAKEIYNVSSTSFMFKGEDNKDKMVTLEVRPWCTNDEAGGTSFGVIFYGS
                     EGWMTFPGYGTYKAYLGKDNKLVKEGNDGNDRYHYQNFIDCVRSRDASKLNAPPIEGH
                     YSSALSHYALTGARVNRVLEIDTAAEQAVNDDEANAMLTRKYREPFVVPEVV"
     gene            14293..15105
                     /locus_tag="PKN92_05365"
     CDS             14293..15105
                     /locus_tag="PKN92_05365"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNZ48254.1"
                     /translation="MHPVSYIAVIIFGLVEGLTEFLPVSSTGHMILLEEWLALPGSPA
                     FAETFRIVVQLPAILAVVLYFWKRLWPFSADKSFSSAVLLWMKIIVAFLPAAVIGLFL
                     DDFLEAWFFNPLSISIALIAGGIVLLLLEKYPAPVQFSRAEEIGFRVALGIGLFQCLA
                     MMPGTSRSAATIIGAMILGASRPAAAEFSFFLAIPTMVGATAYKTVKTGLDLSASEWL
                     LIGAGGLTAFITAWLVVASFMAYIRKRPFAPFAWYRLALGAVILLLLGAGYL"
     gene            15317..16417
                     /locus_tag="PKN92_05370"
     CDS             15317..16417
                     /locus_tag="PKN92_05370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNZ48255.1"
                     /translation="MNFLAARMNSIDASGIRKIFALAAKMKDPINLSIGQPDYDVDQL
                     CKDEACRQIQAGFNAYTQTWGIDELRDAAAEYYDRKFGAPLKNVMITSGVSGGLFLAL
                     MATVNPGDEVICADPYFVMYKHLVNLLGGKAVTVSTFPDFKFTAERLESAITPRTKAV
                     IINTPANPTGVTLNAAELDSLAAVAKKHNLLVISDEIYESLQYDEAPASMVGRYENVL
                     CLNGFSKNMAMTGWRLGYAAGPEQVIQAMNTLQQYTFVCAPSFAQKAAVLALKADMTA
                     KVDAYRRKRDLVYNGLKDVFSCVKPGGAFYIYPRTPDGDGDGFVKKAIENNVLIIPGS
                     VFSDNPSCFRISFAAPDETIRRGCEVLKSLVK"
     gene            16481..18706
                     /locus_tag="PKN92_05375"
     CDS             16481..18706
                     /locus_tag="PKN92_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48256.1"
                     /translation="MVDLTHQDRSSPAQPGMQAKPPAEIPDSYWPFLKISAGTLFILL
                     SAAVAGIMQALPLSSALTLSAGLAAGTALFLIGSLLQLSQMPGGKSLLSGGIVFLFVF
                     LTGLLFSRGSVLLGTAAARSFFNFLLCLLVLILFGSIAWRIRSHSIGTVTALSALYLL
                     HAALPLPDGRACLAVPVLLTCFFAFSLYFWLQDRQGHLLALLILSAASLLWIYAVKAG
                     ALSSPTGITAALTCTYILCQGLILYPCRNRKISAAEALRLCTVNAAAFAFSSGMLLFS
                     PAAPLRIWMSALVLVAAAPLSFLLVQNHPLRLRFRALFGGQILIALGAALLLSWHAET
                     RLILLTALCAGPAFAAWRYPHRSFRILEHLMIAGALILSFFLKNNTSPVMLGPVQLSS
                     LQATLLAASMLFIFFSRLHSTWAHKAETGSLQKEAQQLLSLAHGLAAAFILAFHTILR
                     REDSATLPWILLWQGCFFFGMSLVLSLPALTKGAMVSLLMSHSCYYALPLIYGPVLTP
                     AAVLPGHQASLLLLFTLIAAIAGDTAGRRIYDGAPALPEKIAAALPYGAALFLAGKLL
                     LFKAVFPDYLLFISLIAWIFFLLSENRYRLLPGMKATACLLLTVAFFLCLYRFIAEWK
                     TLYYRPETLLALVMFLFHGLALEYLLGRRLKTLPFLQNLVRCTIPLLLFVVAAAVLRQ
                     WYPMLWYAAALLWLAVFFFTVAQAIHARRYSFCAAAALACLIVGLTVSGLAHLLNSTP
                     L"
     gene            18716..20071
                     /locus_tag="PKN92_05380"
     CDS             18716..20071
                     /locus_tag="PKN92_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNZ48257.1"
                     /translation="MSMNTGHLIFPQDPVAQHAVTICATLQAKGHRALLAGGCVRDLM
                     LGRTPADYDIATDALPEETMALFPHTRPVGIKFGVVLVIVKGTPYEVACFRKDGPYLD
                     GRRPETVSFSDEKEDARRRDFTINALFYDPARQTVLDYVNGIADLHAGILRAVGDPQK
                     RFQEDRLRMLRALRFSVRFSYAIEDRTWRALCREREAITQVSTERIRDELIRLLTEGK
                     ADRALQLLDESGFLKILLPEVAAMKGVEQPPEFHPEGDVFTHTRLALAHLPAQRSEAL
                     TLALLLHDTGKVKTQRFDAGRIRFFGHDHESSVIARRICERFLMPVKTTERIAWLVKQ
                     HMRIHHFQNMRLSKQKQLLREPGFHELLVLARCDALGSNGDMNAVDYAEKMRNALGDE
                     DLSPTPLLTGNDLIALGYKPDRRFRRMLEAVERGQLDGTLADRAEAIALVERIFGPPG
                     T"
     gene            complement(20079..21503)
                     /gene="lpdA"
                     /locus_tag="PKN92_05385"
     CDS             complement(20079..21503)
                     /gene="lpdA"
                     /locus_tag="PKN92_05385"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNZ48258.1"
                     /translation="MADKDKTQLAVIGGGPGGYAAAFHAADLGMDVTLIDVEANPGGT
                     CLYRGCIPSKALLHVAKLITDAREAEEWGLTFAPPEMNLEKMRNRTRQVVDQMTGGLG
                     QLCQARNITYERARASFLDPHTLALNYADGAKKRLAADSVIIATGSRPALFGPLIRSR
                     RLMNSTNALHLEDIPGTLLVIGGGYIGLELGSVYAALGSAVTVAEMTDVLLPGGDRDL
                     VVPLAHRLESRFHQIMLKTRVNTMQETDQGILVNLENEEIGKEDRLFDKVLISVGRKP
                     NSRGLNLFEIGLEIDAQGFIPVDLQRRTAIPHIYAIGDVTGQPMLAHKASAEGKVAAE
                     NIAGRKAVFEPQAIPAVVFTDPEVAWAGITEKQAAAQGIAVAVARFPWAASGRASTMS
                     RPEGLTKILCDPDTGQILGMGITGTNAGELISEGVLAIEMGAVVEDLDMTIHPHPTLS
                     ETVMESAAAVFGTSTHFYRPLKKK"
     assembly_gap    21900..21909
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21912..22826
                     /locus_tag="PKN92_05390"
     CDS             21912..22826
                     /locus_tag="PKN92_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNZ48259.1"
                     /translation="MIIKKLPGFTLIELLVVIAIIGILAAILLPALARAHEAARRSSC
                     QNNLKQMGLAFHMYSDEAKGHMFPSRKIWECDGVTLSNAMIFDGVSMIPEYISDINVI
                     WCPSWTKESSPVERYDQGRGNKNGRVDPCELVKEPFDYTGFVVMDDFNILGFAKIGQS
                     GSGPGGRWEKMEYQDTPWGELAAANANPNAPGRACQEDFRVSLLFNGTQTGEGNTIYR
                     LRQGIERFLITDINNPAASAQAASTVPVMWDHISTNTIDFAHVPGGANVLYMDGHVLF
                     HTYPGTRFPMTVDSARIMGRYNSAFAGF"
     gene            22987..23241
                     /locus_tag="PKN92_05395"
     CDS             22987..23241
                     /locus_tag="PKN92_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HNZ48260.1"
                     /translation="MAKQINVVLPDLGEGTDKVLEATVSGWAAETGAAVKEGDDLLEI
                     VTDKASFVVPSPVSGILSEQCVQEDDAVKVGDLIAVITVE"
     gene            23295..>23628
                     /locus_tag="PKN92_05400"
     CDS             23295..>23628
                     /locus_tag="PKN92_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNZ48261.1"
                     /translation="MEYGVGVIGAGTVGGGVIEILTANRDVISDKTGASVTLRHVAEL
                     KDELLAGLDLSGVTVSKDASALIADPQVHVVCELIGGLEPARRFILEALRAGKHVVTA
                     NKMLLATHG"
BASE COUNT         5230 a         7008 c         6226 g         5154 t
ORIGIN      
        1 aatagccgtc ttcatcgaaa tgacccacat cgcccgtgcg gaaccagccg tcgacaatgg
       61 catcccgtgt cgcctgaggc cggttcagat actgtttcat gacattgtgt ccgcggatga
      121 gaatctcgcc gatctcgcct gttttgccga gctgcccgtc atcgcggaga atccgcatct
      181 gcacgcccca gatcggtttg ccgatggaac cgacccgcac ctcttcgccg ggcagggtaa
      241 aggaggcgac agggcttgtt tctgacagac cgtagccttc cagaatcaca atgccatacc
      301 gttcccggaa acggtggtgt acttcttcgg gcagcgccgc gccgccggag atcgccatgc
      361 gcacagaact gaaatcgtag tcctgccagt tttccggatt gagaatgaaa aagtacatgg
      421 tcggcacaag cgctatgacg gtgaccttgt cccgcgagat cacttccatg gctttttccg
      481 tttcgaaacg gggcagcatg gtcatggtgg cgccgccgat cacgctggca ttcatctgac
      541 aggtctgacc gaaggaatga aagagcggca gagcaacaag cgtcacgtcg tcaggcgccg
      601 ccttcacaat gtgatggcag gcataatagg cgttgaaaaa catattgaaa tgggtgagca
      661 ctgccccttt gggtgcgccc gtggtacccg aggtatagag caccactgcc atgtcgtcgg
      721 gcatggtggg cacagtctcg aagctgctgt ccgccgtcgc cagaagggtg ttgaagtttt
      781 ctcccactgt cggcgtttcc agatcatcgg gtgtagatac aatgatcaga tccttgcagg
      841 attcagcggc gagaaaggct ttgcgcgctt cttcctcgaa gagtttgaag gcgaagaagg
      901 ctttggcgcc cgagtcggaa agatagtgct cgatttcgtg atactgatag agcacgttca
      961 ccgggaccac ggcggcacct gtatggagaa tgccgtaata gattatggga aaatggggcg
     1021 tattcggaat catcagcgca acggtatccc ctttgccgat acctttcgcc gccagaatat
     1081 tggcgacttt gcgcgcaaga gcggcaactt ccgcccaggt aaaacgcaaa tcatcgagga
     1141 tgactgccgt tttatccgga taggtctcag cggtttcatt gagaaaatgg gcgagattca
     1201 gcatagcggg gtgctcctcg ttagcttgag tgctgccagc gccgaccgtt gtattcttac
     1261 gaacagagtc atattacaat actgagtgca ttttttcaat gtgtttgtgt ttttttgaag
     1321 ggaagacggc cgggacagaa gggtaaagat aaagaatttc ctttacatcg aaatcagtgg
     1381 acagaatgga cagaatggac agagtggacg gagcagctct tctctccccc ttttccccct
     1441 tttcttgaaa tgatcacccg gttccggcat tatgtaagga atgcatgtta ctttcagata
     1501 cccgccgtgt tgcgtctctg cggcggtaca acccgatgga gcatccctca gatgaaatca
     1561 gccatgaacc gccgtcattt ccttaaaggc tctttagcgg caggcgccgc cctcacggta
     1621 ccgcagatca ttccggcagc ggctttcggc cagggcgata ccccgccgcc ttccgaacag
     1681 atcaccatcg gctgtatcgg agtcggcagg atgggcagaa acaatctcaa cgacttcatc
     1741 aaactgcccg actgccgtat tgtcgctgtc tgtgatacct atcaggaaaa cctcgctctc
     1801 gctaaagata ttgtcgacac tcattatcag aacaccgact gcgccaccta cggcgacttc
     1861 cgtgacctgc ttgcccgtgc cgatatcgat gccgtcatca tcgctgtaca ggatcactgg
     1921 cacggcatca tcgccaccgc cgccgccaga gccggcaaag atatctactg cgaaaagccg
     1981 ctgggcattt ccatcgccga atcccgtgcc atcctcgatg ccgtacgcag tcacaaccgt
     2041 gtcctgcaga caggcacctg gcagcggtcg ctcaagcctt tccagcacgc ctgcaccctg
     2101 gcgcgcaacg gctatctggg caccatccac acggtggaag tgggatcacc cggccccgat
     2161 tattatgtgc gccacacagg tgatctcgat ggacaccccg tgccggacgg tttcgattgg
     2221 aacatgtggc gcggacctgc ccccgaagtg ccgtacaacc ccggcagggt cgcctggccc
     2281 gactggtacc tgatttacga ttactgcgtg ggcttcatca ccaactgggg cgtccatcat
     2341 ctcgacattg ccaactgggg ctgcccccgc atcggcaggg aacctttcga agtggtctgc
     2401 aaagaaacct tccgcagcga aggcttcacc aacaactcga aaaaatggga tgcccgcttc
     2461 cactatgccg acgggctgac catgatcttt caggacgacc atcagggcga acggggcacc
     2521 cgcttcatcg gtgatgaagg ttgggtgcgc gttgaccgtg acggcatctg ggcgggaccc
     2581 cagtcgcttt tgaaagtgga gttcaaagac accgaccagc ggctcaccga ctccgtcaat
     2641 cacggcgcca acctgctgga atgcatccgt acccgcaaga aacccgtgtc cgatgtggag
     2701 gaaggacata tcgcttcctg cctcggcatg gtcgctgaca ttgccggaag aacagaggcg
     2761 cgccttgcct gggatccgaa aacagaacgc ttcctcaaca atgacgaagc caatgcgctg
     2821 ctcagccggg aactgcacaa cggctggacg ctgtagaccg gaaaataaaa atcagaccga
     2881 aggattcggc acgtagacgg ttcggcactg cttaagataa ttcagggcat gcacctgccc
     2941 ggtcatttcc aggtcatctt tataaacggg gtcgtgccag ccttcaatgt cgatgctgcc
     3001 cttaaagccc gcctgacgca tgatggcaat aatttctgtc cagtttgtat cgccgaaacc
     3061 cgctgtgcgg tgatgcacga acggtctgcc gccgccgatc ccgtaagtac gcaccacatc
     3121 ccactgcacc gttgcatcct tgccgtgcag atgaaacact ttcggcgccc acttgcgcag
     3181 ctgcggcagc ggatcaatca gctgcaccag ctgatggcag ggttcccact ccagcccgat
     3241 atggtcgtag ggcagcgcat caaaaatcag ctcccacgcc gccgggttgt gcgcaatatt
     3301 ccagccgccc gtcttccagt tgccgcccat ctcgcagttt tcaaaggcaa tgcgaaggcc
     3361 ccggtccgcc gcccgctgcg caagcggttc aaacacctcc ttcagccggg gaatgctctc
     3421 gtcaatgggc gcatcgatga cacggcccgt aaagcccgcc acaatatcac agccgaaaag
     3481 atgggcagta tccagcagct ccacccagga ccggagcgtg tcttcgtcct gcggacggcc
     3541 gcccagagga ttcccgtaaa ccgccacact gctgatcacg gcatccgtgc cggcaagtgc
     3601 ctcgcgcacc tcccccgcca gccgtttcag atcggtgtcc ccgatcgttt tccagaaggt
     3661 gatctgaaaa gattcaaaac catgctgcac cacctctttt atatacgccg gcggattcat
     3721 ggagctctga acaagcgtcc cgatacgtat gtccgtcgat ggatccgggg aagatcctgc
     3781 tgccgggtca ccggaaactg ccgaagcagc cgcctgcacc ggcgggatca ccgcagcagc
     3841 acctgccgca aaaagtgccg tatctttcag aaaagaacgt cgtgtacggg acgcagtcat
     3901 gatcgggtct ccttttttaa aacagaaaga gtactccgcc gattatacgc ccgcaggcgc
     3961 tgcaaaacaa tgcaggaagg aaaagcgggg cgacgccagg aagacgacgt ccacgctgtc
     4021 cactctgtca acggttttgg tgtggatctc agttctgtga aacgaaagcg acccttaccc
     4081 tggttaagat tcctgcctgc gcaggaatga cggaggaaag agcaggaatg acggaggaac
     4141 gaaaacgtcc actctgtcca ctctgtccac tctgtcaacg gttttggtgt gggctgcagt
     4201 tctgtgaaac gaaagcgacc cttaccctgg ttaagattcc tgcctgcgca ggaatgacgg
     4261 aggaaggagc aggaatgacg ggggaaagag caggctttcc ggacgggaaa gctaaaacaa
     4321 tgcttccagt tcgatcaggg catggcgggc acggtgatca agatcaagaa ggtgcatccg
     4381 catttcggcg acttcctgct gtgcggcaag cacttcaaaa aggctgacgc cgccgagggt
     4441 cagtgctgtt tccagcgccc ggcaggcatc ttccagtttc gggaaatttt tctcccgaac
     4501 cagatgcgcc tctttcagcg ctgctttgcc ttcaccgagc agggtcagcg cctgccggca
     4561 gtcttcctga atcttcgcct gccgggctgc ttccagccgg cgggcgcgtg cacgtgcccc
     4621 tgcaatagca ccctgattgc ggtcgaaaag aggcaggtca aaactgacgc tgaagtccac
     4681 agcattctgc ttttccgccc gctcctgccg ccagcccaca tgtacttccg gctctggcag
     4741 cgcttcccgt ttcagcgctt tcacttcgcc cgccgccgct tccgcttcaa gttcgagagc
     4801 acgtgcagca ggggcgctgc cgagccgttc cgccacaaca gcggcatcag gaaaagcctg
     4861 ctcctccgga agaagcgttc cctccagtac cggcttcgcg cccggcggca gtgcgcagag
     4921 cacagttaac gctgccagag cagtttcctc acggatgcgc agcacttcca gctccgcttc
     4981 ctgatggttt tcttccgccg ctgcccggag ccagtccatt tctgcgatat cacccgcttc
     5041 aaaacgctgc cgggtcattt ccgccatggc cgccgcgttg gcaagattat tttcagcaat
     5101 gcgccgggac tcctgcgccg ccagtgcctc gtaaaaagcc atttcagcca gtgccgtcag
     5161 ctccttttca cggagaagcg cctgctgtcc cgctgcggca acacctgctt cggcggcttt
     5221 ccgtgcgaga gcacgtgccc cgaaaagcgg gatacgctgc gagaccccgg caagccagtc
     5281 gccgccccgg ttgccgtgga gcccttccgc tccggcttcc agttccgggt tgggccagag
     5341 cccttcctgc agcgcatgac cgcgggcttc ctccacggct tccagcgctt cctgaatgcc
     5401 gggatggatt ttaagcactc tgccgacaac ctccgcagcc gtaagggtga ttatttcttc
     5461 ctgcgctaaa gatccgcccg ccagaagaag cacagcggca gcgaccatgg tcaagatttt
     5521 catcttcaga tttcctttca tgttcagggc ggctcaggcc gttgtttctt cttttgctgc
     5581 ggggcgcccc aaccgcagat aaagggcagg caccacaatc atattcaaaa aggtcgccga
     5641 gagaaggcct ccgataataa ccacagccat gggactctgg atctcattgc ccggtgcttc
     5701 atgggcgagc accagcggaa tcagcgcgaa gccggtggac aaagccgtca tcagcacagg
     5761 cacaatccgt tccactgaac cgcgacgcac tgcctcggta aagtcggtca ccgcttcttc
     5821 cagctgcagg tgccggatat gattgaccag catgatgcca ttgcgcgcag ccacaccgaa
     5881 gacgctgata aagccgataa tggaagccac agacagcacg ccgcccatga gaaatacagc
     5941 cgccacaccg ccgatgagcg ccagaggcag acttgccatg acaaaaagag catcccgtcc
     6001 gtttttgaat gccatgaaca gcagggcaaa cacaccgaca agcaccacgg cgccggaggc
     6061 aagaagcctg cctgatgcgg cggcggcact ttcaaactgt ccgccgtaat gaacagcata
     6121 gccttctttt cccgccacca gcggatcgat ttccgaacgg atatcccgga ccagagacgt
     6181 cacgtcccta cccgaaggat tgcaggtgac gaaaatcttg cgttgcacct gatcgcgggt
     6241 tatcacattg gggccgctgc cccggaacac agacgccacg gcattgagcg gcaccagcgc
     6301 acccgaagcg ctgcgcagcg gcagcttttc cagcgatgcc agtgtcgcat cgccctcttc
     6361 cgccagccgc agcacaagat cgtaagcgtt gcgcccttca aaaatgcggg agaccggcgc
     6421 accgaaaagg gcggcacgca gcgtcagccc cgcctcttca aaggaaatgc cgtgccgggc
     6481 gagaagatcc cggtcaaaac gcacgcgcac ctccggcaca tccgtctgag gctccacggc
     6541 aatatcggcg gcacccggta ttttctttat ccgcttctgc gcctcttcag caagttcgcg
     6601 cagcgtatac aggtcttcgc cggtgaattt gatggcgatg ctggaacggg caccggagag
     6661 catatggtcg atgcggtggg atatgggtcc tccaatcccg taactgatgc ccggaatcct
     6721 gtcgagtctg ccgcgcactt cctccagcat ttcctcctta ctccgttctt tcaacaggta
     6781 ggtcgcctcc atttccgagc cttcaatccc ctgggcatgc tcatcgagat cgcctctgcc
     6841 cgttttgcgt gccaccgaac taatctccgg cacttccatc agctgctgtt cgatctgcag
     6901 gccgatacga ttggactcct ccagcgaagt gcccggcagc gtattcgcca ccacgttcag
     6961 cgagccctca ttgaaatcag gcaggaacga ccggcccatg aaggaaaagg caacgacaga
     7021 taccacaaat gcagcgacta ccggaagcgt cacgaaccac ggatgacgga gaaccgggcc
     7081 gagcaggacg ttatacgcac ggcgcaccag ctcggcaaca cggggttccc ggggactctg
     7141 cacaataccg ctgccgggaa gaaggacggc agccagcgcc ggggtcagtg tcagcgccac
     7201 cagcagcgat gcaaagatcg cgacaatgta ggaagtcgcc agcggctgga gcagcctgcc
     7261 ctccacattt tccagcgtat aaagaggaag aaaaaccacc gtgatcagta ctgtcgcaaa
     7321 aacaatggcg gaacgcactt ccgagctcgc ttcatagaca atacgcaaag gcgacacgtt
     7381 cccgtcggca agatgtactc tgcccagacg cagccgccgt attgtatttt ccacatcaat
     7441 gaccgcgtca tccatcagcg agccgatggc tatcgccagc ccgcccaggg tcattgtgtt
     7501 gatggatccg ccgaaaaact gcaccgacag cacggcaata atcagagaga ggggcagtgc
     7561 agtcagggta atcagaagtg cccgcccgct gagcagaaaa aatgccatga tcagcactac
     7621 gataaccatg ccgtcccgga tcgcattgga aacattacgc acggcaacac ggatgaaatc
     7681 cgcctgcttc atgaggttgg tgtccagcgc catgccttcg ggcagcttcg cccgttcatc
     7741 ggcaaacacg tcttccagca cctgatccag attcagcgta ttcgcctgag gctgtttctg
     7801 aatgatcagc agcaccgccg gtttgccgtt tgccgagccg gtaccccggg tcggcgccgc
     7861 acccagccgc acctcggcaa catcttccac gcagaccgga attccccgct tggttgccac
     7921 caccaccttg ccgatgtcgg tcgtattctg cagtctgccc aaacccgtaa ccagatattc
     7981 cgtgccccgc tctgaaataa aaccggcggg aatattcata ttgttcgagg cgagcgcttc
     8041 cgccacttcc gcaacagtca catcccgagc tgtcaggcgt tccggcttca gcgccacctg
     8101 atactggcgg tcgccgctgc ccaccaccgt tacctgcgcc acaccgggca ccgcaagcag
     8161 ccgccgcgcc agcacaatct ccgcatagtt ctgcaggtcc gtcatggaac ggttgtctgc
     8221 atgaagggcg gcaatctgta tttcgcccat gagcgaggtg ggcggcgcaa gaatgggcgg
     8281 cataatacct tcaggcagtt ccggtatcgt cagctgcaca cgctccgcca cgatctgacg
     8341 cgccacattc atctcttcgc cccagtcaaa ctccgaccag acaatggaaa agcccggcat
     8401 ggaagcagaa cggactcgcc ggattcccgg ggcaccgttc aaagtcgttt ccagcggaaa
     8461 ggtcaccaga ttctccacct cggaagggga ataccccggc gcctcggtca gcaccaccac
     8521 cgtgggcgcc gtcagatcgg gaaatacgtc cacaggagta tgaaagcaga gatacagccc
     8581 gaagatgccg aatacaagcg aaagggctat caccagatac cggttgctta cggaccatcg
     8641 aatcagttta tccatcatgg ccgcagctcc ttaatgcaca tgggcggaac cgacgccgcc
     8701 tcctgccaga cctgcaagac gcaccgcata ggcaccgcgg acaaccaccc ggtcgccttc
     8761 ctgaagaccg tccagcactt ctgtcatcac accgtcactg atgccggtgc gaatgggacg
     8821 cttctgaaaa tgctcgcccg tcagcatgac gtaaaccgtg ggaatgccgt tttcatccac
     8881 gagggctgaa tggggtatct gaagcgcatc ctcttctttt tttccatccg ccagcagggt
     8941 cagcgtcatg ccgatgcgaa gccgttcctc ctggttgcgc gtataaaaaa tgaggggaac
     9001 agtccggctt tcttctttta cctgcggctg aagccagggc tctctttccc cttccggcgc
     9061 ttccatctca aagagctcgc cgggaaaagc agggagttca tagcaggcat gaaggggccg
     9121 ggacagtgcc ggagccagca cttcaggcac ctgcccttcg atcagcacct catcggcatt
     9181 gaccacttca atgagcatct gcccgccgtc cacctgtgtg ccgggccctg ccgcaagccg
     9241 ggtaatcctg cctgtgatgg gagatcgtat ccaggccgca accccttctt cttcccgcga
     9301 ttccagagtg ggcagtgccg ccagcgcttt tctgacctgt gcctccgtct gttccgcttc
     9361 ccgaagcagt gcctgagcgg aagcaagcgc actccgctgc tcttccactt cccgctgtga
     9421 acccgctttc gccacttgca agcgctccgc acgatccagt gccgaagcat gctgctgcac
     9481 gagtgcccgt gcccgttccg catcgccccg cgcctgagcc gcctgtacgg caagctccgt
     9541 gcgctgtccc tgcaggctgt ggatattcga ttcccgtgcc aacgcctcac cgccctgcag
     9601 cgtcggaagc acacccgcca gaacctgccc ttcttccact tcctcgccta tgccgggaaa
     9661 agagccgccc tccggaggaa ggagcctacc gctcaaagga gcagtcagcg cggcgcggga
     9721 atgaggcgtc tcctgcacgg tcgccggaat cctgtaccgt tccaccagtc cgccgcgaaa
     9781 ggcttgtgcc gtcaccagcg gcagcaccca ctgctgttct ttcgaaaaca aaatggcgtt
     9841 gccgggcaca tcttcactgc tggcatagac cgttccgtcg gcgtgcacgt gatcactgtc
     9901 atctccgtgg tcgtggtcgt gatcgtgatc ttctccatgg tcatgatcat gatcgtggcc
     9961 ttcctcttga tcgtgggcat ggtcgtgaac cgcatcagga ccgtccgtat ggccgtggtc
    10021 atggatgtcc agatcgggga agatcagctg aacctcccca tccgccagag acagctttac
    10081 cgtaagatcc caatgaccgg gtgccggaaa agtcaccgtc gttttataat ttcccgggcg
    10141 gaccgcaggg acacgaacat ccaacgtact tccgtccgat tcctttttcc attgaagcac
    10201 cgcgtcgccg gcgtctaccg gcaatccgct gtccagacgg gtgatatgaa gggtgaactc
    10261 ctgcggcgtt cccgcatcaa gatgaccgaa ttcaacatgc ccttccagct gatccgtcca
    10321 cagcgtgaca gaagattctt cttcctcacc atgctcgtga ccgtggtcat ggtcatgtcc
    10381 gtgatcgtgg gtgtgttccg ttactttagg gagggagcag cctgcgataa aaagaggaag
    10441 cagcgctgcc agaaaaaggg ggcgtactga cgtcctgccc ataagagtgg atatcatgaa
    10501 atagatcctt tcctgaatga catttctgca aaataagaca gaaccataca aaatcaggaa
    10561 agaagatcag ctgataagag gcagattcct gccggagaag gacggagagc gaacactatc
    10621 ggatgaatcg cccggaatag cagcagggga gcgtgtgccc aaagaccaga ggttcagtaa
    10681 ttctcctgca aaagacgggc tgacaccgcc gggcaccatt ttcgtctcgg gaagttcaaa
    10741 ggcgcgtgtg ccatgaagat gaaactgacg gcatccgtgg gctgaggcgc tcagaagcat
    10801 ccctgatgat ccaccgccat gatccgcacc gctgtcacaa caggccgata caaaggaaga
    10861 tgccgcaggg cagcgaccgg gatgatggtg ggcgcagaaa cccaaagaat acaccgccac
    10921 cagcagagac agcaccgtcc tgccccagag cactgctccc gggcaaaaaa cggctgctat
    10981 cttgcggata agactcatag gcatacttcc attatcataa aaagaacgca tttccggcaa
    11041 aagctattcc gataatttta catcgggtga aaaaatagtg attgcagtat attcactgtt
    11101 agagagcgtt aatccgtttt tcaaagtctg ctaagccccg ttcaatggct tcaaaggtgg
    11161 gaatatcgcc gaaaagcatt gcctgcatct tccggtagtc tacctgtaat tcctcccgat
    11221 gatattccga aggaagcagg caaaaactgc cgggacgggc tgtttcatag cttgcccagg
    11281 gtgaacgata aaaccgctgt ttgaatgcga ctactttttc cagcaaatcc agcctttcca
    11341 gtgccctttt tgcgacagag ctctgtgtca tgcgatagag atcgtaatag tgcctcgagt
    11401 aacggggcgg cacttgcttc tttgaattgc gatgggcctc ctgatgaaga atagtggcct
    11461 tttcccagaa ggtacgttcg gcacttatgg tacggatcac agtgaaagtt tttttaaatg
    11521 cacccggaaa atgctctgcc gcataaggat aaattttttc agaagcgtta ggaagccagg
    11581 atgccatagg acctatttcc aatctgactt ccggttgaat agcattcagg gaaaatgctt
    11641 tcggataccg gattagaata tcctctttcc agggaataat ctcaaaaggc acggcagcat
    11701 gcagcggcag ttcacggcga agagcaggcg caagttcatc ggcaagaaac tgcgcagtgc
    11761 ggagattcgc ctcctgacat aaaagagcct gttgagtaat actcctttcg tgaagcggtt
    11821 cgtcacgggt atatcccagc acctgccagt caagtaccag atcaatgtct tcagaaaaac
    11881 gatttataag cccgaatgcc tttgaaaggc ttgttccgcc tttgaacgca aaattctttc
    11941 cccatttaca gtgattaaaa agaaagtcca gaagcacaca aacccagaaa tccttctccg
    12001 caatggcagg atgcactgcc atagacatag ctgtttcata gaaaaggtcg cggcgaaacg
    12061 ctgcgccttc tcctgcaatg ttaagcatgt attccttcct tttccgcgag gcacttgatc
    12121 gccgactgca tccagatggg gagatggcgc gtttcactga ccgcctggcg gcactccgtt
    12181 accgtcagcc tgtgacgaag gaagccgaga acttcatcag tgacggcgtc ccgtcccaaa
    12241 gctttcagtg cctgcacgat aagaattgtc tttgatgcca ggcctgaggt ttgtctgccg
    12301 gcacaatgct tgagcaaaac atttccgccg ggccattgat agcattttgt cgggccatta
    12361 ctgcaatact gccattgggc gggcacctga gtggacagac ccagcaggtt cagcgctgtt
    12421 tctccggtag gaatcagcgt ccagccgtga gcacgggcaa tggcatgagc aacagcatcg
    12481 ggccggggag tggcaggccc ttttttcacc gggctgtagg ccggaaaatc gtaaacaccg
    12541 cgaaggatgc gccggagcgt cccggtatcc gccagcctgc ccagactttt ttttgcagcc
    12601 tcatagcctg ctaggtccaa aaagtcttta gccgtaaacg cggcaccttt ttctgctttc
    12661 cgcattcttt tcaggatgta atctcctgtc gttttcatct gattatttcc ttatttgtcc
    12721 caaattataa ctattattcg ggacaaatgc aaaccgggta cccgttttcg aaaccactcg
    12781 gtctttgacg gtgtcatata tttattgaat ggtttattta tgctttgtta ggataaatga
    12841 cagtattgtt taacacatag gagattaata cgcatggact ctctcacacg ccggtctttc
    12901 atgaagacct ctgcagcagg tgcaggcgcc gctgcttcca ttcttctggg cacctccaaa
    12961 acatcctggg caggagccaa cgaccgtgtt cgtattgcgg tgatgggcat caacggccgg
    13021 ggcagaagcc atctgggcgg attcagcaag ctgaaaaatg tggaaatcgc cgctatctgc
    13081 gatgtggatt cacgtctttt tcccgctgtt gtgaaagacc tcacaaaaaa cggcaaagcg
    13141 ccccgtaccg aacaggatat ccgggtactt ctggaagatc cggatattga cgccatttcc
    13201 attgccacac ccaaccactg gcactctctt gcgacaatct gggcatgtca ggcaggcaaa
    13261 gatgtatatg tggaaaaacc gatgacccac aacatctttg aaggccggaa agtggtggaa
    13321 gccgccgcca aatataaccg catcgtccag cacggcaccc agcttcgaag caaccccggc
    13381 ttccaggaag ggatcaatga actgaaaaac ggtctcatcg gcgatgtcta tatggcacgc
    13441 tgcgtctgct ataaatggcg ccccgacatc ggaaaagggc atcccggcaa cccgcccgaa
    13501 ggactcgact ggaatctctg gcagggtccc gcacaggaag aacccttctt tgtcaatgac
    13561 aaaggcgaag gcatttatgt gcactatttc tggcactggg tctgggcgta cgggaacggc
    13621 gacatcggca atcagggcgt gcatcagctc gacgccgcac gctggggtct gggcgtcaat
    13681 gtgccctaca gagtcacttc catgggcggt atgttcctgt gggatgatgc caaagaaatc
    13741 tacaatgtct cctccacttc ctttatgttc aaaggtgaag acaacaaaga caaaatggtg
    13801 accctggaag tacgtccctg gtgtaccaac gacgaagcag gcggcacctc cttcggtgtt
    13861 atcttctacg ggtctgaagg ctggatgact ttccccggct acggcaccta taaggcgtat
    13921 ctgggcaaag acaataaact ggtgaaagaa ggcaacgacg gcaacgaccg ctatcattac
    13981 caaaatttca tcgactgcgt ccgcagccgc gatgcatcca aactgaatgc acctcccatt
    14041 gaagggcatt attcctccgc cctttcccac tacgccctta ccggcgccag ggtcaaccgg
    14101 gttctggaaa ttgataccgc cgccgagcag gccgtcaacg atgacgaagc caacgccatg
    14161 ctcacccgca aataccgtga gcccttcgtt gtgcccgaag ttgtctgaac gccagtatct
    14221 tttctaacat aagacggagc ctgtgaggca tcaggtctca caggctcttt gctgttcgag
    14281 tcatcgccgg agatccatcc tgtgtcatat atagctgtca tcatattcgg gcttgtggaa
    14341 ggactgacag agtttcttcc cgtaagcagt acgggccaca tgattctgct ggaggaatgg
    14401 ctggcgcttc cgggcagccc tgctttcgca gagactttcc ggatcgtggt tcagctgcct
    14461 gccattctgg cggtggtgct gtatttctgg aaacgcctct ggcctttttc cgccgacaaa
    14521 tccttcagct ccgcggtgct gctgtggatg aaaattattg tcgcctttct gcccgccgcc
    14581 gtgatcggtc tttttctgga cgattttctc gaagcctggt ttttcaaccc gctcagcatt
    14641 tccatcgccc tgattgcagg cggaattgtg ctgttgctcc tggaaaaata tccggcaccc
    14701 gtacagttca gccgggcgga agagatcggc ttccgcgtcg ctctcggcat cgggcttttt
    14761 caatgccttg ccatgatgcc gggcacctcc cgctcagccg ccaccattat cggcgctatg
    14821 atcctgggtg cctcccgtcc tgccgccgcc gaattttctt ttttcctcgc cattcccacc
    14881 atggtcggcg ccaccgccta taaaacggtg aagaccggac tcgacctctc cgcctccgag
    14941 tggctgctca tcggagcggg cgggctcact gcttttatta ccgcctggct ggtagtcgca
    15001 tcttttatgg cttatatccg caaacgccct tttgcccctt ttgcctggta ccggcttgcg
    15061 ctgggtgctg tgattcttct gctgctgggt gccgggtatc tgtaagtgaa atattcttcc
    15121 gcggtcaaag gaacactgcc acggcggatt actccggcat atgcgaatct gccctgtctt
    15181 tttgaagcat cctgcttttc cgtggtacat ttatggttcg aggtgttccg cagagttatg
    15241 aacagggatg caaatctgcc gttacttttt caattgacag cgggcggcac tgtgccgccg
    15301 caacaaggat cattccatga actttctcgc agcacgcatg aactccattg atgccagcgg
    15361 tatccgtaag attttcgccc ttgccgccaa aatgaaagat cctatcaacc tcagtatcgg
    15421 ccaacccgat tacgacgtgg atcagttgtg caaggatgaa gcctgccgtc agattcaggc
    15481 gggtttcaac gcctatactc agacctgggg catcgatgag ctccgtgatg cggcggcgga
    15541 atactatgac cggaaattcg gtgccccgct gaaaaatgtc atgatcacaa gcggtgtgtc
    15601 gggcggtctt tttctggctc ttatggcgac agtcaatccc ggcgatgagg tcatctgcgc
    15661 agatccttac tttgtcatgt acaagcatct ggtgaatctt ctgggcggaa aagccgtcac
    15721 cgtcagcacc tttcccgatt tcaaattcac ggctgagcgt ctggaatccg ccattacccc
    15781 cagaacaaaa gcagtcatta tcaatacccc tgccaatccc acaggtgtca cgctgaacgc
    15841 tgcggaactg gacagtctgg cggccgtggc aaaaaaacac aatctcctcg tcatcagcga
    15901 cgaaatttat gaatcccttc agtatgacga agcgcctgcg agcatggtgg gcaggtacga
    15961 gaacgtgctc tgcctcaatg gattttccaa aaacatggcg atgaccggat ggcggctcgg
    16021 ctatgcggca ggcccggaac aagtgattca agccatgaac accctgcagc agtatacctt
    16081 tgtctgtgcg ccttcctttg cacagaaagc ggccgtcctt gctctgaaag cggatatgac
    16141 cgccaaagtc gatgcctacc gccgcaagcg cgatctggtc tacaacggac tgaaagatgt
    16201 cttttcctgt gtcaagccgg gcggtgcttt ttacatctat ccccggacac cggacggcga
    16261 cggcgacggc ttcgtgaaaa aagccattga aaacaatgtg ctcatcattc cgggcagcgt
    16321 cttctccgac aacccctcct gtttcagaat ttccttcgcg gcaccggatg aaacgatccg
    16381 ccgcggctgt gaagtgttga agtcattggt aaaataggga cacgctttct gccggggccg
    16441 gcggaaagac tgccgtgccc ggcggaaagg aaaggaagct gtggttgatc tgacccatca
    16501 ggatcgaagc tctcccgctc agcccggtat gcaggcaaaa ccgcccgccg agattccgga
    16561 cagttactgg cctttcctta aaatatctgc cggaactctt ttcatcctcc tgtcagcggc
    16621 ggtggcgggc atcatgcagg ccctgcccct ttcctccgca cttacactgt cagccggtct
    16681 tgctgccgga acggctcttt ttctcatcgg cagcctgctg caattgtctc agatgcctgg
    16741 cggaaaaagc ctgctcagcg gcggtatcgt ctttctcttt gtcttcctta caggactgct
    16801 tttctcacgg ggatccgtgc tgctcggcac agcggcagcc aggtctttct tcaactttct
    16861 gttatgcctg ctcgtgctga ttctcttcgg ctcaattgcc tggcgcatcc gaagccactc
    16921 gatcggcacc gtcactgcac tgagtgccct ttatctgctg catgccgcat tgccccttcc
    16981 cgacggtcgt gcctgcttgg cggtacctgt gctgcttact tgctttttcg ccttttctct
    17041 ttacttctgg ctgcaggaca gacaggggca cctgctcgca ctgctgatac tcagtgccgc
    17101 atctctgctg tggatttatg ccgtaaaagc cggggcgctc tcctccccga caggaataac
    17161 ggctgcccta acctgtacct atatcctctg tcaggggctg atcctttacc catgccggaa
    17221 ccggaaaatc agcgccgccg aagcactccg cctttgcacc gtaaacgctg ccgcctttgc
    17281 gttctcttcg ggaatgctgc ttttttctcc tgccgccccg ctgagaatct ggatgtcggc
    17341 gctggtgctg gttgcggcag cgccgctttc tttcctgctg gtacagaacc acccgctccg
    17401 gcttcgcttt cgcgcacttt tcggaggaca gattctcata gcgttggggg cggcgcttct
    17461 gctcagctgg catgcggaaa cgcgtcttat tctgcttaca gccctgtgcg ccgggcctgc
    17521 ttttgccgcc tggcgctatc cccaccgcag tttccggatc ctcgagcatc tgatgatagc
    17581 gggggcactc atactctctt tttttctcaa aaacaacacc agcccggtga tgctgggacc
    17641 ggtgcagctt tcctccctgc aggcgacgct gcttgccgct tcaatgcttt ttattttctt
    17701 ctccaggctc cacagcacct gggcgcataa agcagaaacc ggaagtctgc aaaaagaagc
    17761 acagcagctt ctttctcttg ctcatgggct tgctgccgcc tttattcttg ctttccacac
    17821 cattttgcga cgtgaggaca gcgcaacact gccctggata ctgctctggc agggctgctt
    17881 cttcttcggc atgtccctcg ttctgtcact tccggcactg acgaaagggg cgatggtctc
    17941 cctgcttatg tcacacagct gctattatgc actgcccctg atatatggtc ctgtcctgac
    18001 cccggcagcc gttctgcccg ggcatcaggc ttcgcttctg ctccttttca cgctcattgc
    18061 agctattgcg ggcgatacag caggcaggcg aatttatgac ggtgcacccg cactgccgga
    18121 aaaaatcgct gccgcactgc cctacggcgc cgctctgttt cttgccggga agctgctgct
    18181 ctttaaagcg gtatttcccg attatcttct tttcatcagc ctgatcgcct ggatcttttt
    18241 tcttctttca gaaaacagat accgcctgct tccgggtatg aaggcgacgg cttgcctgct
    18301 gctgacagtg gcatttttcc tgtgcctcta tcgttttatt gcggaatgga aaacactcta
    18361 ttaccggccc gaaacgctgc tcgctctcgt gatgtttctt tttcacggtc tcgccctgga
    18421 gtaccttctg ggcaggaggc tgaaaaccct gccttttctg cagaatcttg tacggtgcac
    18481 gatccccctg ctgctctttg ttgtagcggc agccgtgctg cgccagtggt acccgatgct
    18541 gtggtatgct gcagccctgc tgtggcttgc cgtatttttt ttcacggttg cgcaggcgat
    18601 ccacgcccgc cgctacagtt tttgtgcggc tgcagcactt gcctgtctca ttgttggact
    18661 cactgtatcc gggcttgcgc atttgctgaa cagtaccccg ctctaggaag ccgccatgag
    18721 catgaataca gggcatctta tcttcccgca ggatcccgtg gcgcagcatg ccgtgactat
    18781 ctgcgccacc ctgcaggcaa aggggcaccg ggcgctgctg gcgggcggct gcgtgcgcga
    18841 cctgatgctg ggcagaacac ccgctgacta cgatatcgcc accgatgcgc ttccggaaga
    18901 aaccatggcg ctttttccgc atacccgccc tgtgggcatc aagttcggtg tggttctcgt
    18961 catcgtcaaa ggcacgccct atgaagttgc atgtttccga aaagacgggc cgtatctgga
    19021 cgggcgccgc ccggaaacag tttctttttc cgatgaaaaa gaagatgccc gccgcaggga
    19081 tttcaccatc aatgcactgt tctatgatcc ggcgcgtcag actgttctgg attatgtcaa
    19141 cggaattgcc gacctgcatg cgggaattct tcgtgccgtg ggggatccgc agaaacgctt
    19201 tcaggaagac cggctgcgca tgctgcgtgc cctgcgcttt tccgtgcggt tcagctacgc
    19261 catcgaagac agaacctggc gcgccctctg ccgggaaaga gaggctatca cacaggtcag
    19321 cacggaacgg attcgcgacg aactgatccg cctcctgact gaaggaaaag cggacagggc
    19381 actccagctt ctggacgaaa gcgggtttct gaaaattctg ctgcctgaag tggcagcgat
    19441 gaagggcgtt gaacagccgc cggaattcca tcctgaaggg gatgtgttca cccatacccg
    19501 cctcgcactc gcccaccttc ccgcacagcg ctcagaggca ctcacgctcg ccctgctgct
    19561 tcatgatacg ggaaaggtaa agacacagcg ctttgacgcc ggcaggatcc gcttcttcgg
    19621 gcacgatcat gagagcagtg tgattgcccg gcggatctgt gagcgcttcc ttatgcccgt
    19681 gaaaaccacg gagcgcattg cgtggcttgt gaagcagcac atgcggatcc accattttca
    19741 gaatatgcga ctgagcaaac agaaacagct gctgcgtgag ccgggctttc atgagctgct
    19801 tgtgctggca cggtgtgacg ccctaggaag caacggcgat atgaatgccg ttgactatgc
    19861 ggaaaaaatg agaaacgctc tcggagacga agacctgtcg ccaacgcccc tgcttacagg
    19921 aaatgacctg atcgctctcg ggtacaagcc ggatcggcgt tttcgcagga tgcttgaagc
    19981 cgtggaacgg ggacaactcg acggaaccct tgccgaccgg gcggaggcaa ttgctttggt
    20041 cgaaagaatt ttcggcccgc ccggcacctg aggcatgcct atttcttttt caacggacgg
    20101 tagaaatgcg tactcgtgcc gaagaccgcc gcagcagatt ccatgaccgt ttcgctcagc
    20161 gtaggatggg gatgaatcgt catatccaga tcttccacca ccgcgcccat ttcaatcgca
    20221 agcacgcctt ccgaaatcag ttcaccggca ttagttccgg taatgcccat gcccagaatc
    20281 tgacccgtgt ccggatcgca gagaatcttc gtaagccctt ccggacggct catcgtgctt
    20341 gccctgcctg atgccgccca gggaaaacgc gccaccgcca cagcaatgcc ctgcgccgcc
    20401 gcctgttttt ccgtaatccc cgcccaagcc acttcaggat cggtaaacac caccgccggg
    20461 atcgcctgag gctcgaacac tgcttttctg cctgcaatat tctccgccgc cactttccct
    20521 tctgctgagg ctttgtgtgc aagcatgggc tgccccgtca catcaccgat cgcatagata
    20581 tgcggtatgg cggtgcgccg ctgtaaatcc acagggataa agccctgagc atcaatctca
    20641 agccctattt caaaaagatt caggccccgc gaattgggct tgcgacccac actgatcagc
    20701 accttgtcaa aaagcctgtc ctctttgcct atttcctcgt tctcgagatt gaccagaata
    20761 ccctgatccg tttcctgcat ggtattcaca cgcgtcttaa gcataatctg gtggaaccgg
    20821 ctctccaggc ggtgcgccag aggcacgacc agatctcggt cgcccccggg aagaagcaca
    20881 tctgtcattt cagcgacagt cactgccgag cccagtgctg cataaacaga gcccagctca
    20941 aggccgatat acccgccgcc gatcacaaga agcgttccgg gaatatcttc aagatgcaac
    21001 gcattggtgg aattcatcag gcggcgggaa cggatcagcg gaccgaagag cgccggacgc
    21061 gaacccgtgg caataatcac cgaatccgct gcaagacgct ttttcgcacc atccgcataa
    21121 ttcaacgcca gcgtatgggg atcaagaaaa gacgcacggg cgcgctcata tgtaatattg
    21181 cgcgcctgac aaagctgccc caaaccgcct gtcatctgat ccactacctg gcgtgtacgg
    21241 ttccgcattt tctccaaatt catttctgga ggcgcaaagg tcagacccca ctcctccgcc
    21301 tcccgggcat ccgtaatcag tttcgcaaca tgcagcagtg ccttggacgg aatacaaccc
    21361 cggtaaaggc aagtaccgcc cggattggcc tccacatcaa tcagcgttac atccataccc
    21421 agatctgcgg cgtgaaaagc agccgcataa ccgccagggc cgccgccgat aactgccagc
    21481 tgtgtcttat ccttgtctgc catcttgtcg ctttcattga aaaggatgca gccccttcag
    21541 ggaccgcaag gaaatgaagt taagaagaga aacctgttcc gaaatcctct aacaacataa
    21601 acagttgccg gtcgccacta ttcccggacg gaaaatgcac agaccgactg aaacaaaggg
    21661 ctctccggca accctgaagg acgccgccca cttgcatatg ataccccagc agcaggggta
    21721 aatggcaaca gcatgctttc gtatccgcat gtgccggcgc ctctcaaaaa gttgcaaatc
    21781 gatcggtcgc tggtatagga taatcatagt atacatatgt ttatgtatgc atgcgtttgt
    21841 tgtgttgggc taaacaccca taaactctga gtgcagagag gcaaagaaag aacaacagcn
    21901 nnnnnnnnng catgattatc aaaaaacttc ccggcttcac cctcatcgaa ctcctggtag
    21961 tcattgccat tatcggtatc ctcgccgcca tactcctccc tgctctcgca cgggcgcacg
    22021 aagccgcaag acgctcctca tgccagaaca acctcaaaca gatggggctc gcttttcaca
    22081 tgtacagcga cgaggcaaaa ggccacatgt ttccgtcgag aaaaatttgg gagtgtgacg
    22141 gtgtcacttt gagcaacgcc atgatttttg acggtgtcag catgattccc gaatacatct
    22201 cggacataaa cgtgatctgg tgtccctcct ggacaaaaga gtcttctccc gtagagcgct
    22261 atgatcaggg gcgcggaaac aaaaacggcc gcgtagaccc ctgcgagctc gtaaaagaac
    22321 ctttcgacta tacaggcttt gtggtcatgg atgacttcaa tattctgggc ttcgctaaaa
    22381 ttggtcaatc gggctcaggc cccggcggac gctgggaaaa aatggagtat caggatacgc
    22441 cctggggtga actcgcagcc gccaacgcca atcccaatgc gccaggaaga gcctgccagg
    22501 aagacttccg ggtttctctt cttttcaacg gaacccagac aggcgaaggc aatacaatct
    22561 atcgtctgcg ccagggcatc gagcgcttcc tgatcacgga tatcaacaac ccggcagcaa
    22621 gtgcacaggc cgccagtact gtgcccgtaa tgtgggatca catcagcacc aacaccatcg
    22681 actttgcaca tgtccccggc ggtgccaatg tcctctacat ggacggtcac gtgcttttcc
    22741 acacataccc gggcacacgc ttccccatga ccgtggacag tgcacgcatt atgggccgat
    22801 acaacagcgc ttttgccggc ttctgaaaaa gaatttcacg ctaaccgatg ctatggttgg
    22861 gctgaagagg gttcttttgg cataatattt cagttcaggg agcgttccgg cagcagtgtc
    22921 gctttttttg gatacagcgc cggacagcct atcgtttaac tgaagccagg gaggaaccga
    22981 taagccatgg caaaacagat caatgttgtg ttgcctgatc tgggagaagg tacggacaaa
    23041 gttctggaag ctacagtatc cggctgggcg gctgaaacag gtgcagcagt aaaagaaggc
    23101 gatgatctgc tggagattgt aacggataaa gcaagctttg tcgtgccgtc tcctgtctcg
    23161 ggcatcctgt cagaacaatg cgttcaggaa gacgacgctg ttaaagtagg cgatctgatc
    23221 gccgtaatta cggtagaata gtgtctgcag catacgggcg gaaatcccga agtaaacgat
    23281 aaaggcaaca gactatggaa tatggcgttg gtgtcatcgg tgccggtaca gtcggcggcg
    23341 gcgtaattga aatccttacg gcgaacaggg acgtaatttc ggacaagacc ggagcatccg
    23401 tcaccttgcg gcatgttgcg gaactcaagg acgagctgct cgcaggactg gatctcagcg
    23461 gcgtgactgt cagcaaagat gcttccgcac tgattgcgga tccgcaggtg catgtggtat
    23521 gcgagctgat cggcggtctc gaaccggcgc ggcgctttat tctggaggca ttgcgggcag
    23581 gcaagcatgt ggtcaccgcc aataaaatgc ttctggctac ccacggtc
//
LOCUS       DAOPWB010000039        23607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2143_length_23607_cov_5.359375, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000039 DAOPWB010000000
VERSION     DAOPWB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 23607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23607
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2143_length_23607_cov_5.359375"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKN92_05405"
     CDS             <1..271
                     /locus_tag="PKN92_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48262.1"
                     /translation="SCLRRNDEGGGRYDGRKQQWRRGVDITSEGSNDSVVCRTDGGGM
                     PREVFPQRIRVTVIVMARLCANTETGVPRGAAFSLWCGMDYYPLL"
     gene            631..2910
                     /locus_tag="PKN92_05410"
     CDS             631..2910
                     /locus_tag="PKN92_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HNZ48263.1"
                     /translation="MQNNPQNEERLSPWWKNGVFLVLIAGFSVLIWLAVKSYQDAPPI
                     PDQIRNEQGDILFTGDDIRSGQEIFLRQGLMNNGTVWGHGAYLGPDFSTSYLYHQTND
                     AYELLAKQKYQKSWSDLNEEQKEGIRENVAKDSRVNRYDPSTGVLTLSAAEAYSYEQQ
                     QAVWRKFFSEPHQNFGLPEKYIDDAEELRLLTAFFFWTSWASVTNRPGKEFSYTNNFP
                     YAPAVNNVPTHGTLLWSALSLIALLGGTGLALFVFGRFDFLGWQQKDHLHPQLLPGEA
                     STGQKAVLKYFVIAALLFLVQATIGGGLAHYRAEPGDFYGFDLSSLLPSNILRTWHLQ
                     LAIFWVATSFVAGGLFLAGAVNRNEPKKQALGIHVLFWALVAIVVGSLGGEWLGIKRL
                     LGSLWFWFGHQGWEYLELGRAWQVLLAVGLVIWLVLIARCILPVRKDPERGQIASLFL
                     YAAIAIPLFYLPAMFFGSRSHFTVVDMWRFWIIHLWVEGFFELFATTMVALMFFLLGM
                     VSKKTALRVVYMDAILYLGAGIIGTGHHWYWTGQGHITMALSAVFSALEVVPLTLLTL
                     DAWDFISLSRNDCDECGKKVPIPHRWTFYFLMAVGFWNFLGAGVFGFLINLPVVSYFE
                     VGTLLTPNHGHAAMMGVFGMLAVALMVFAFRESSTDAEWKKLEKLVGLSFFGLNIGLV
                     LMVVMNLFPGGVFQLYDVLANGYWHARSPEFLTQGLMRTIEWLRMPGDVVFLGLGCAP
                     LALAGALTYVNSWRRSSAE"
     gene            3080..4066
                     /locus_tag="PKN92_05415"
     CDS             3080..4066
                     /locus_tag="PKN92_05415"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNZ48264.1"
                     /translation="MDFSELNPKRKVVVIGAGSVGATFCYALAQSGLADEIVLIDHNK
                     DLARGQVLDLAHGQSFFPPVTIRVGDQQDYADATLLVMTAGARQQPGETRLQLLRRNA
                     SIVRQVARDIALANSPGMLVIVSNPVDILTKVAAEETGWRRGRVLGSGTVLDSARFRS
                     LLSHNCGIDIHNIHAYILGEHGDSEFAAWSMTHVAGIPIDEYCTSCRMCTPTGKQDEW
                     LGVRKSIEEQVRNSAYHIIDYKGSTYFAIGLALVRIAGAILRNENSVLTVSTLLEGEY
                     GVSGLCLSVPAVVSGRGVERIMEARLNPEEQKALARSAAVLRASLNELEGNE"
     gene            4079..6091
                     /locus_tag="PKN92_05420"
     CDS             4079..6091
                     /locus_tag="PKN92_05420"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ48265.1"
                     /translation="MKTVLCGLSALLLVLCCSLAPADITGRVTDEESAGLAKARVFAE
                     PGVEKEVIEGVVSADGTFVIPGDFFGSVGIFAWAPGHAFSGTHLTLAAGDDPGHLQIR
                     LGQEVRLSGKTVDEKGHAIAGARLITLAITAPVKVGIPLSKLEPFGFPLSRSDDNGLF
                     HLSGLPKNGQVALKFEHPLYAREAVTGLASGSGDVRVVLHRGVALRGTVLITGTDRPV
                     SGAVVIAHNTQPPHDTAATATDSTGSFRLLLKPGVYLLQAHSAGRISPGMQRVDLTGE
                     LPEVSLRLPLSETGIITGSAHDAKTGQPLAGVRFFLETRGQAGGVARTGRQGQFQLNA
                     PEGSNTIRLDPVPGYQAPEPAALQVMVPPSGTLKLPGFWLAPTPDYRVQVLQSDTATP
                     VPGACAALLQPRQFGWQYADARGSLFLQFGAQPKDGRLIGFVEHPQEDAGALFSLVPP
                     LKADASVALHAFGRVTGRVVNSKGQALPGVVVGSLYADDSLEEPLTLWRQVTDKDGYF
                     DWRWAPAGVPQRCVAVLGSLSAEAAKDFNPAPGEKIDLGEIAVPSTQGSALKKPVHWT
                     ELPWQSGPALSSRPDRAAVAFFCTVAEAPLFMEALSGFVEKLSGIGVDTLLAVSGDYQ
                     PERAAVPVYRSETPETVTRLYTPSGALFLETPGIPPFSAFRAAVKE"
     gene            6096..7949
                     /locus_tag="PKN92_05425"
     CDS             6096..7949
                     /locus_tag="PKN92_05425"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNZ48266.1"
                     /translation="MIASFLRSYMTVFCLVCLFPAAAFSFSVKGFTLDAHGNAVSGAA
                     VFCTQDREARKAVSGADGSYRFDNISVRMMALAVYKEGYALGGQTCLPIGDLELNLIL
                     PPPGEVLIRVINNNFLPIAGARVTSMRVNDLFHVPVDELVEAGFPFLRSDDKGLLNLS
                     CIPAGGYVKLTLAHHHYADSSVAYLPVDSRRRDIVLYEGVRLRGRITASGKAVARARV
                     TLFQQGVGEQKVFAEALSDPEGYYHLRAPEDSYLLVAHHREYASPQPQPVQLRSGEEA
                     ASEDIVLTQPHVIRGTILYPDGKPCPGAGILFRVAETVFEEAVSDSTGTFELRIGRPD
                     GIVRVVPPPGFMTEILADIPVQMGEAMKTTLPPIKLKALPVIRGKTKFPDGVEPEKLY
                     LHSLDLPAPIHLITEKDGSFEIHFLYQPEQKNIAFRLEHPFRFLRCDFSVALDQSAVT
                     EAVLETFEPDLERRPPEQARNDMERLLGKEAPAFACTDWFNSHPMTLEQLKGKVVVLT
                     FWGGFDNSVFAMNRLQEMQAAWELYKDQGDVVVLGIHDASNEADEIAEYVRSYGLTFP
                     VGKDDDPFFSFVNYGINYIPQTVMIDKQGKVQYTQVENRLFELIKALRRRS"
     gene            8001..9128
                     /locus_tag="PKN92_05430"
     CDS             8001..9128
                     /locus_tag="PKN92_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ48267.1"
                     /translation="MMRCFFQKRTRLTALALCLILSVGCGQMTDKDRIRVAKVGERYI
                     TRGDLFKIIREMPDNVRPMIRNRSDLVRILNQHIDNSIKRKLGIQFAKEGRVAVDREL
                     AREMFFRDSGDEEESLRNMWSMEVPPPGVITPLMSVYGLTPELLQFNKDQVEEGTDRM
                     IIKLQGEQAVEVLAAEAFKDGRITLDEGEVEREYGFMKEQFIVPEYLEFKGIRFVDAP
                     DAAARASALRERLQAGESFDSLLEEYMEKEKEDNTDYVITAGIINDPQKVHLRAFWEA
                     ASGAQPGDIVGPVFLRASQQRTEDSSGKTVITEQPPYYLICKVIASRPQSLLPYEQAR
                     PQVIVPLIKAKMMHLLREDNKVEIYEDKLPDPSHMQGPAAV"
     gene            9194..10285
                     /locus_tag="PKN92_05435"
     CDS             9194..10285
                     /locus_tag="PKN92_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNZ48268.1"
                     /translation="MRRLITMSLLLLVCVGSAQADLILPHFETNVEIYRLFGEEHPGP
                     YKHPASITQLENGDYYVAYYGGENEYSAETAVYGSRCKQGKKKWSAPKIIAKTPFQGV
                     GNPVVWQAPDGVVWLFYNNQYGDTWSNARVKAKISLDGAKSWSDSFMLCMEEGSMVRG
                     QPIVLDTGEYLLPLYNEKGDDREFTDSSSVSYFLRYNPKTQEWTESKNRIRSPEGNLQ
                     AQVVQISATDLFCFMRRGGGYGPTEDGWMLRSDSKDGGFSWTDAVRTDFPNPNSAVEL
                     IKLKNGHLLLVYNDSMSDRTPLTLAVSTDNGETWPFRRMIAGGDNDFAYPYAIQGSDD
                     KIYLIYTTNSRTSIMLAVFDERAVTEYEP"
     gene            10442..11389
                     /locus_tag="PKN92_05440"
     CDS             10442..11389
                     /locus_tag="PKN92_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNZ48269.1"
                     /translation="MKRKGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCANN
                     LKQFGLSFKMYANEAPGEKYPPVQHQTYCSPCFGVLLTPLCVAVYPEYISDPNIYVCP
                     SSASHKIQDMYYDNGTTILQFIGQDGDTSYNDWYKANFSYSYFGFAYDLCDSVPANLV
                     DIDPALISILQITSPGFTVPANTKAPAQLQRQWLKIFQENNMGAMPYNRRGPWPPFEE
                     DTKGVSPVGNGGSDVVYRLREGIERFLITDINNPAASNMAQSDVAIMWDLVSSKVDRF
                     NHIPGGANVLYMDGHVSFLKYPSEKAPVTKEVALVSSIF"
     gene            11542..12330
                     /locus_tag="PKN92_05445"
     CDS             11542..12330
                     /locus_tag="PKN92_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ48270.1"
                     /translation="MPEQDDQFKGIVEGNRRTRLSCCGFSDRFDPFRMSALPHVAAFY
                     QDVFSELTKGAPYNRILDVGCGTGLYFEWLCALTKRLDGIEPSQEMLAVARKFVLARE
                     LQGVYLQEGTAESLPFDDETFDAVVVLDALHHTGDREGAIEEIRRVLKPGGLLLVLEP
                     NIRSPLIAVMHFFHREERAALRANKPRRLRAFLASRFSERSWRGACQIVAELRGIRGL
                     LAKIYLLFVRHSLSETWYPRQIWLGMKPPLSKPLASRRKKKRNR"
     gene            complement(12369..13151)
                     /locus_tag="PKN92_05450"
     CDS             complement(12369..13151)
                     /locus_tag="PKN92_05450"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HNZ48271.1"
                     /translation="MNNSGRPQQLEFDWGQEDPTFLCPPELHACPSTEIKARLEHQTG
                     LQIDLSITECSQLMLAFRIIEEKKKVLVHMHSMFLCASEDVINHIAKWLLNPNSKRSC
                     SFLQEHIRKYEIIARQNQGATHRYKIIHQGRFFNLGEIFKQVNQSSFNNEVTATITWS
                     RHTKGKARSIRLGSYRSCCHLIRINPRLDQKFVPLFFIRHVIYHEMLHSIFPRQKTAS
                     GRNSIHSKEFRALERKSPDYEAATQWEKTPGNIGRLLGRQKP"
     gene            complement(13144..14097)
                     /locus_tag="PKN92_05455"
     CDS             complement(13144..14097)
                     /locus_tag="PKN92_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48272.1"
                     /translation="MKRYSYWLVVATVAFIALLVVGWRYLDPPNQGFRIYTKRMVSYT
                     LELLENDPTVQLPETRACVRLVAASAEASWLNTPEALYILALQYERENSTENAELWFR
                     EVIRLAPGWSWPYVNLGILLARTDAGRLEEAAELLTAAIALQPDWVRPYNSLSVVLRS
                     LGRLEEAEAMAIAAITLEPQDVAAHNNYANLLAVMGRYEEAEEHYRYAMENAPENSKP
                     PYNLACLYCARGLDEEALDYLAFAITLSSDARLDAASDPCFSALRDNPYFQELIYGEP
                     EAVPAEETGLEEGAASEPPEETEADAEEGDPGESTEASEHE"
     gene            14309..16291
                     /locus_tag="PKN92_05460"
     CDS             14309..16291
                     /locus_tag="PKN92_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ48273.1"
                     /translation="MSDRKNTSRRGFLKAAGGSGMAAGYSLFIRADRAEGADAPLPPG
                     VEKAVAAVPTSPGAPPPHEPNMVQVDLHCDFLVAGGGMAGVCAALAAARTGARVVLVQ
                     NRSRLGGNASSEVRMHIVGASCHRSRLGWRESGILEELRLEDAKHNPQWSFELWDLLL
                     YDKVVSEPNITLLLDTSVFRAVVSDNRIREVWARSDLTETLYRIEAPLFADCTGDCRL
                     GVEAGAAYVVGHESRDKYNEVRAPKTAGTETLGSSILFMAKDYGYPIPFTPPPWARKV
                     QAEQLRLRKIRSWEYGYWWIEWGGEDDAIRNNEAIRKELLSIVLGVWDYIKNSGDHPD
                     SANWGMDWIGMIPGKRASRRLVGPHVLTEQDLLGMNGSFEDAVAVGGWPFDDHPPGGF
                     DAVDLVPSDSQNIKNVYGIPLRALYSVNIANLFMAGRNISASHVAFTSTRVMGTCAVE
                     GQAIGTAAAFCAARNMTPQALYEDKELLKQYQQQLLRDDQTIPGLANEDAADLARRAS
                     YVRASHTVEGSRPENIINGLVRDMGEEWNNRWGAELQAGTAWIELGWESPHTISLIQL
                     THDSGFSRELTLSGEQSVRTPQKRSAQPETSRDYRLLYRETGGGEWKELVQVEGNYQR
                     LRRHAFSPVQAQAVRLEVTATNGCDIVRLYEMRCYA"
     gene            complement(16327..17415)
                     /locus_tag="PKN92_05465"
     CDS             complement(16327..17415)
                     /locus_tag="PKN92_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNZ48274.1"
                     /translation="MNFGILQFSEQYAPRLWGGSRLAALGKMLPADQCIGEAWLIADH
                     PQCESRVISGERKGDTLRTLMEEWGDRLLGTAAHPAANHRFPLLLKLIDAGDMLSVQV
                     HPDDAAAKALHEPDGGKTEMWHILNAAPDSRIISGTRHGVTADSFRTSLPEGGCGKLL
                     REFPVSPGDSIFVPAGAVHAIGAGILLAEIQQNSDITYRLYDWGRVDVRGIPRELHVD
                     KGLQVARFGEDTSGRMAPLRCTGETCVSEYLCASRFFAAQRITLYHKTRFTMTGSSFR
                     IILALQQQLVLEDQFGTASLQPGEAALVPAWLPWWEASGTGSFLCFYVPDATADIIEP
                     LRRHGYSNRQILSLGEGDPGRMLQSLLQ"
     gene            17661..18614
                     /locus_tag="PKN92_05470"
     CDS             17661..18614
                     /locus_tag="PKN92_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48275.1"
                     /translation="MNQHIIWVLSLCFIASVSDGKELFEDASLRHGFELSAVASTKKP
                     LTVSQIFVTHPSFKGPRWRLAQWGTRYSLVTAEEEKLPDGTRSLSNAGKTVLLHPGGL
                     AGEGVTLRVNGGAEYPDRLRRFGEPWPHLLVEQRLRAVACSDWQSLTFHLEFFVEQCI
                     PVRDTAQDPSLHTAQVGVFFTVHNLNKKSADFRDMIWFGLPVYDVRFGISPGHQALDI
                     GKNDATGKFICTLSGDRFYSEPVLPGKWHTLSADLLPLLQDALDASKEHGHLTHSELK
                     DLQFTSFNLGWEVPGSYDCAIRLRRLSLKGEASDEVPGRRR"
     gene            18618..19388
                     /locus_tag="PKN92_05475"
     CDS             18618..19388
                     /locus_tag="PKN92_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNZ48276.1"
                     /translation="MSKTLCIHLIEGEEGCRFAVERKAVAVIVDALRASATAALLLEK
                     GTDKICAVREVEEALEMKRHWPDAVLFGERGGRPPAGFDYGNSPEEAACAGGRSVIFT
                     TTTGVSRLISASGAFAAVMATTVNGAALLSFLQQTDAAEAVIIPAGLAGDPGFDAQED
                     WTAAVWLAGLCLEHPAPGAFFSLGEGAEAFRRYQERISKEGLSGLFHSAPHAARLRAI
                     SMEKDIDFCARTNICSALPIACGRRGTGVLLRAWRPAG"
     gene            19492..19725
                     /locus_tag="PKN92_05480"
     CDS             19492..19725
                     /locus_tag="PKN92_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HNZ48277.1"
                     /translation="MSDNTSRFSADMTVADAMKLHPRAAEVFAAFHLGGCAHCHINAF
                     ETIEQVCGAYGVEVSVLLEVLEGLFTNEATSAS"
     gene            20003..23590
                     /locus_tag="PKN92_05485"
     CDS             20003..23590
                     /locus_tag="PKN92_05485"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNZ48278.1"
                     /translation="MTAKAFGWKSGLFSLVLLTVCCSLPALAKPTIKINGPNPHYWEC
                     SVAYDDQGAIATDSNGVDITRDIVVDGLYNLDTSILNAEYDIVYRVVDSTGASATASR
                     RVIIVDTIPPIFALEKDLDNVTGEVPLRLIPVSFDPDKKPVPPLWLIWLKQQFGPFDD
                     QEGEEEHPKPDEFELTLADIANWPEWAQRMAMVWYFFPIGRGVVYEIPDWGANPMEWR
                     CSGKTWEDPGVKVFDACDIAVPLDEVYVFMVEVYIEDNYVWEEELWNGKYTDLNPNQL
                     KSPGSKRNLAGYRLYYYAPDTSRNIGVAVRDVLPRYGGWLTLAGDPNFSVNCGDDSIW
                     PTILQLEKLDRYYDSCEGDASYKISKSGVVNWNVPGTYHLVYKAGNHIAKRTIQVNDA
                     MPVITLLDKNQAPVSSPEDSAVTLNWCEYIKLDPSAEGAVIVDEYGLQRWWPLRPDEG
                     FYATDGCDDGYVISSQTQAYGSGEISKQLLKLAASGIAEAHTVYERIWYEARDPRNNR
                     AYKERPAIIVYVPPILSFKESGGSSWIPSQVYLEANKFQCGRSITLSQGKFDLLGCNV
                     LNVPAYVTDTGGLDLENPEVGIYSVTYRSFPDDPARGLALTVQVEVVDNQEPGVILDT
                     FGTLQNNEWFIPCFDTTTQDAYTLTDGCSPATDLKLQKYVWRLLGDLATDAVPDVVRD
                     KKGRPVDYGANDLFKKDPGSYIIVYVVTDAEGNTNLPLASDGLPNVFHSNGGVNVFAS
                     PGVLSKRFEESVRIVRTQAATRLVPDLPMDDEDGFYLVSCTEDINTLLNNSVINNCFA
                     DRVDRFVWKFQNDGSIKRERTGEPSEYVPEQLKGRLGDYVAVLVSYLDRQTQPLLNEK
                     VFADILNEKGFLIDPDAPYLMRFRIQDTDPPVMTLKGDAVVTLLCGTKYVELGCSAVD
                     ECEGVLDSIKQEPLPKAIDTSKPGSYTITYSAEDKSGNTASVTRTVNIVDLTADDVEI
                     VMLGEAEIALECGSHFTDPGVEVRYKEKKLNVYSRSVDYFGVIGETTGTVRVNYYATT
                     PAGTELLVGQRTVIVRDTTAPVIQLNGESALKLTQGSVYFERGARVYDSCSGQVDLRV
                     DGQVDTGKIGVYTITYGASDASGNTATAVRTVTVEKEKANAEGETPEVVEGEIEGESP
                     VEGEEDDDCVGCFGRTGCCGARKMPKNLLGEYLLLGVSMLALALFRRRG"
BASE COUNT         5223 a         5911 c         6710 g         5763 t
ORIGIN      
        1 ttcctgcctg cgcaggaatg acgagggggg aggcaggtat gacgggagaa aacagcaatg
       61 gcgccggggg gtggacataa cgtcggaagg gagcaatgac agcgtcgtgt gtcggactga
      121 tggaggaggc atgccgcggg aagtatttcc gcagcgaatc cgagtgacag ttatcgtcat
      181 ggcccgcttg tgtgccaata cggagacggg cgttcccagg ggagccgctt tcagtctttg
      241 gtgtggaatg gactattacc cgcttttgtg atcaatagtg aaatttcctt tagagacgca
      301 tatttttcct ctaattacct gagacttata tgggtcccat accaaaatac tctggcttaa
      361 aacataaaat ccgccagcct gggaacgcca atctccgtat tggcaggggc ttttgtggtc
      421 tgtgtttatg ggagtatatc gcaggacata aacccgtggc gaatccgagt gactgtcatc
      481 ccggatattt tgttttttac ctcatttgtc tgtactataa ttcattcact gccggctttg
      541 ctggaaaata aatttgttct atgcatataa gttccatttt aacagcctga aagtagtaaa
      601 gggcggggct gcaaaaagaa aggaaagagc atgcaaaaca atccgcagaa tgaggaacgg
      661 ctgtcgccct ggtggaaaaa cggtgttttt cttgtgctca tcgccggttt cagcgtcctg
      721 atctggctgg cagtcaagtc ctatcaggac gcgccgccta tacccgacca gatcagaaat
      781 gagcaggggg atattctttt taccggcgat gatatccgtt ccggtcagga gatctttctg
      841 cgccagggac tgatgaacaa cgggactgtt tgggggcacg gtgcgtatct gggcccggat
      901 ttttccacca gttacctgta ccatcagacc aatgatgcct atgagctcct ggcaaagcag
      961 aaatatcaga agagctggtc ggatcttaat gaggaacaga aggaaggcat ccgtgaaaat
     1021 gtggcaaagg attccagagt caaccgctat gatccttcca ccggcgtgct cacgctgagc
     1081 gctgccgaag cctacagcta cgagcagcag caggcggtat ggcgcaaatt tttctccgag
     1141 ccgcaccaga attttggact tcccgaaaag tatattgatg acgccgaaga actccgcctg
     1201 ctcacggcat ttttcttctg gacatcctgg gcgtccgtga ccaaccggcc cggaaaagaa
     1261 ttttcctata ccaataactt cccttatgct ccggcggtga ataatgtgcc gacacacggt
     1321 acactgctct ggagcgcatt gagtctaatc gcactgctgg gcggcacagg tcttgccctt
     1381 tttgttttcg gccgttttga ttttctcggc tggcagcaga aagatcatct tcatccccag
     1441 ctgcttccgg gtgaagcctc cacaggacag aaggctgtcc tgaaatattt tgttattgcc
     1501 gcactgctct ttcttgtgca ggcaacgatt ggaggagggc tcgcccacta ccgtgctgag
     1561 cccggcgatt tctacggttt tgacctttca tcgctgctgc ccagcaacat tctgcgtacc
     1621 tggcatctgc agcttgccat tttctgggtg gccaccagtt ttgttgccgg tggtctcttt
     1681 cttgccggcg ccgtcaaccg caatgaaccg aaaaagcagg cgctgggtat tcatgtcctt
     1741 ttctgggcgc tggttgccat cgtggtcggc agtctcggcg gcgaatggct gggcatcaaa
     1801 cgcctgctcg gcagtctctg gttctggttc ggccatcagg gctgggagta tctggagctg
     1861 ggccgtgcct ggcaggtgct gctagcggtg gggcttgtaa tctggctggt actgattgcc
     1921 cgctgcattc ttcctgtgcg caaagatccc gaacggggtc aaattgcgtc gctgtttctt
     1981 tatgcagcta ttgccattcc gcttttttat ctgcctgcca tgttctttgg cagccgctcc
     2041 cactttaccg ttgtggatat gtggcgtttc tggattattc atctctgggt ggaaggtttc
     2101 tttgaacttt ttgccacgac catggtcgcc cttatgtttt tccttctggg tatggtatcc
     2161 aaaaagacag cgctccgtgt ggtttatatg gatgcgattc tgtatctggg tgctggtatt
     2221 atcggcaccg ggcaccactg gtactggacc ggccagggtc acatcaccat ggcgctttca
     2281 gccgttttct ccgcactgga agttgtgccg ctgacgctgc ttacgctgga cgcctgggat
     2341 ttcatatcct tgtcccgcaa tgactgcgac gagtgtggca aaaaagttcc cattccgcat
     2401 cgctggactt tctactttct catggcggtg ggcttctgga atttcctggg tgccggtgtc
     2461 ttcggcttcc ttatcaatct gccggtggtg agttattttg aagtaggcac gctgctgacg
     2521 cccaaccatg gtcatgccgc catgatgggt gtattcggca tgctggctgt ggcgctcatg
     2581 gtattcgcct tccgcgagtc atctacggat gcggaatgga agaaactgga gaaactggta
     2641 ggcctttcct tcttcggact taacattggc ctggtgctga tggttgtgat gaatcttttc
     2701 ccgggcggtg tatttcagct ttacgatgtc ctcgccaacg gctactggca tgcccggagt
     2761 ccggaattcc tgacgcaggg gctgatgcgc accatcgaat ggctgcgtat gcccggcgac
     2821 gttgtatttc tcggtcttgg ctgtgcgcct ctggccctgg caggggcact tacctatgtg
     2881 aacagctggc gccgctcttc agctgaataa atgccagcgt ggcccggact gctgtccggg
     2941 tgtccggttt ggaaacagaa ttacgggcgc ttacccgcac actgcgggta agctgtccgt
     3001 atttctgcgc aggctgtcgg aatgccgctg aaatcaggcg tgtttcgcgg agtggggcag
     3061 ctgtttttca aggaactgta tggatttttc tgagctcaat ccaaaacgga aagtagttgt
     3121 aatcggtgcc ggttcagttg gcgccacttt ctgttatgcc ctggcgcaga gcgggcttgc
     3181 cgatgaaatt gtcctgattg atcacaataa agaccttgcc cgcgggcagg tgctggatct
     3241 ggctcatggc cagtcatttt tcccgcccgt tacgatccgg gtaggggatc agcaggacta
     3301 tgccgatgcg acccttctgg taatgacagc aggggcaagg cagcagcccg gggaaacgcg
     3361 cctgcaattg ctccggcgca atgcttccat tgtgcggcag gtcgcgcggg atattgcgct
     3421 tgcgaattcg ccgggcatgc tggtgattgt gagcaatcct gtggacatcc tgaccaaggt
     3481 ggcggcagaa gaaacgggat ggcggcgagg ccgtgttctg ggctcgggca cggtgctgga
     3541 cagcgcccgg ttccggagtc tgctcagcca taactgcggt atcgatatcc acaatatcca
     3601 tgcctatata ctgggcgagc acggcgacag tgaatttgcc gcctggtcga tgacgcatgt
     3661 cgcgggcatt cccatcgatg aatactgcac atcctgcagg atgtgcacac ctacaggcaa
     3721 acaggacgag tggcttggtg tccggaagag cattgaagag caggtgcgca attccgctta
     3781 ccacattatt gattacaagg gttccactta tttcgccatc ggtctggcat tggtgcgaat
     3841 tgccggagcc attctccgca acgagaacag cgtgctcacg gtttccacat tgcttgaagg
     3901 tgaatacggg gtgtcagggc tttgcctcag tgtgcctgct gtcgtgtcgg gacgcggcgt
     3961 tgagcggatc atggaggcaa ggctgaatcc ggaggaacag aaggcgttag cccgctcggc
     4021 ggcggtattg cgcgcttcgc tgaacgaatt ggaaggcaac gagtagaaga ggagtgatat
     4081 gaaaactgta ttgtgcggac tgtccgcgtt gctcctggtt ttgtgctgtt ctttagctcc
     4141 ggcggatatt accggccgtg ttaccgacga ggagagtgcc ggtctggcga aggcacgggt
     4201 gttcgctgag ccgggcgtgg aaaaagaggt catcgaagga gttgtttccg ccgacggtac
     4261 ctttgtcatt cccggagact ttttcggcag tgtcggcatc ttcgcctggg cgcccgggca
     4321 tgctttcagc ggtacgcacc tgacactggc ggcgggtgat gatccggggc atctacagat
     4381 tcgtctgggg caggaagtgc ggctcagcgg aaaaacggtg gatgaaaaag ggcatgctat
     4441 tgccggcgcc cgtcttatca cgctggcgat taccgctcct gtcaaggtgg gtattcctct
     4501 atcaaaactc gagcccttcg gttttcctct ttcccgttct gatgacaatg gactcttcca
     4561 tctgtccggt cttccgaaga acggacaggt cgctctgaaa tttgagcacc cgctgtatgc
     4621 acgcgaagcc gtgaccgggc ttgcttcagg cagcggtgat gtgcgcgtcg tgctgcaccg
     4681 gggggttgca ttgcggggaa ctgtgctgat taccgggaca gaccggcccg tctccggtgc
     4741 ggttgtaatt gcgcataaca cccagcctcc ccatgatacg gcggcaacgg caacagacag
     4801 caccggaagt ttccggctgc ttcttaagcc gggtgtatat ctgctgcagg cgcacagtgc
     4861 gggacgcatc agcccgggca tgcagcgggt ggatcttacg ggcgaacttc ctgaagtttc
     4921 cctgcgtttg cccctttccg aaacaggcat catcaccgga tcggcgcatg atgcaaaaac
     4981 ggggcagcct cttgcagggg tgagattttt tctggaaacc cggggacagg cgggcggcgt
     5041 ggcgcgtacc ggcagacagg gtcagtttca gctgaatgcc ccggaaggca gcaatacaat
     5101 ccggctcgat cctgtgcccg gctatcaggc accggagccc gccgcgctgc aggtgatggt
     5161 accgccctcg ggtacgctga aactgcccgg cttctggctt gcgccgacgc ctgattacag
     5221 ggtgcaggtg ctgcaatcgg atactgcgac tccggtgccc ggcgcctgcg cagcactact
     5281 gcagccacgg cagttcggct ggcagtatgc cgatgcccgc ggctccctct tccttcagtt
     5341 tggcgcacag ccgaaagacg gacggctcat cggttttgtc gagcatccgc aggaagatgc
     5401 cggcgctctc ttttcgcttg taccgccctt gaaggctgat gcgtcggtgg cgctgcatgc
     5461 cttcggcagg gtgacgggcc gggtggtgaa cagcaaagga caggccctgc cgggagtggt
     5521 ggtcggttcg ctctatgccg atgacagcct cgaagaaccc ctgacgcttt ggcgccaggt
     5581 tacggataaa gacggatatt tcgactggcg ctgggcgcct gcaggggtac ctcaacgctg
     5641 tgtggctgtt ctgggaagcc tttccgccga ggctgcgaaa gattttaatc cggcgccggg
     5701 ggaaaagatt gatctgggag agatcgctgt cccgtccacg cagggatcgg cactgaaaaa
     5761 gcccgtacac tggacagagc ttccctggca gagcggcccg gcgctatcgt cccggcccga
     5821 tcgtgcagct gtcgcttttt tctgcacggt tgcggaggcg ccgcttttta tggaggcgct
     5881 ttctggcttt gtggaaaagc tgtccggtat cggcgtggat acccttcttg ccgtgtccgg
     5941 cgattatcag ccggaacgtg ccgcagtgcc tgtgtaccgg tcggaaacac cggaaacggt
     6001 gacacgtttg tacacccctt ccggagcact ttttctggaa acacccggta tccctccgtt
     6061 cagcgctttc cgtgcagctg tgaaagaata gccctatgat cgcttccttt ttacgttctt
     6121 acatgaccgt tttctgtctg gtctgtcttt ttcccgcggc tgccttttct ttttccgtaa
     6181 aaggtttcac acttgatgct catggcaatg ccgtgtcggg cgcagcagta ttctgcaccc
     6241 aggatcggga agcacgcaaa gcagtgagcg gtgcagacgg cagttatcgt tttgacaaca
     6301 tttccgtgcg catgatggcg ctggcagttt ataaagaggg ctacgctctg ggtggtcaga
     6361 cctgtctgcc cataggtgat ctggaattga atctgatcct gccgcctccc ggtgaggttc
     6421 tgatccgggt aatcaacaac aattttctgc ccattgccgg tgcccgtgtc acttccatgc
     6481 gggtgaatga tcttttccat gtgcctgtcg acgaattggt tgaggcgggt ttccctttcc
     6541 tgcgcagcga tgacaaaggg ctgctcaacc tgtcatgcat tccggcaggc gggtatgtga
     6601 agctgacgct ggcacatcac cattacgccg attccagtgt cgcctatctg ccggtggatt
     6661 cacgcagaag agatattgtt ttatatgaag gcgtacgcct ccgtggcagg attacagcgt
     6721 cgggaaaagc cgttgcccgt gcccgggtta ccttgtttca acagggggtg ggcgaacaga
     6781 aagtatttgc cgaggcgctc agtgatccgg aaggctatta tcatctccgg gcccctgaag
     6841 acagttactt gcttgttgcg catcaccggg agtatgcatc accccagcca cagccggtgc
     6901 agcttcgttc aggcgaggaa gcggcgtctg aggatattgt cctgacgcag ccccatgtga
     6961 tccgtggtac gattctttat ccggatggga agccctgtcc cggagcaggc atcctgtttc
     7021 gtgtggcaga aactgttttt gaagaggctg tttcagacag tacaggcacc tttgaattac
     7081 gtatcggcag accggacggt attgtgcggg ttgtaccgcc gcccggattc atgacagaga
     7141 ttcttgcgga tattcctgtt cagatgggag aagccatgaa aacgaccctg cctcccataa
     7201 aactgaaagc gctgccggtt atccgtggaa agacgaaatt tcccgacgga gtcgaaccgg
     7261 aaaagcttta tctgcattct ctggatctgc ccgcgcccat tcatctgatc acagaaaagg
     7321 acggcagttt cgaaattcat tttctttatc agccggaaca gaaaaacata gccttcaggc
     7381 tggagcatcc cttccgtttt ttacggtgtg acttttctgt tgccctcgat cagtccgctg
     7441 tgaccgaagc tgtgcttgaa acctttgagc cggatctgga gcggcgcccg ccggaacagg
     7501 cccggaatga tatggagcgg cttcttggaa aagaagcccc ggcttttgcc tgcaccgact
     7561 ggttcaacag ccatccgatg acactggaac agttgaaggg gaaagtagtc gtactgactt
     7621 tctggggcgg ctttgacaac agcgtttttg cgatgaaccg gctccaggag atgcaggcgg
     7681 cgtgggaact gtacaaagat cagggagacg tggtggtgct cggtattcat gatgccagta
     7741 acgaagcaga cgaaattgcc gaatatgtgc gcagttacgg gctgactttt cccgtcggga
     7801 aagatgacga tcccttcttc agttttgtca actacgggat taactacatc cctcagacag
     7861 taatgattga taaacagggc aaagttcaat atactcaagt ggaaaaccgg ttatttgaac
     7921 ttattaaagc actgcggcga cgctcatgat gtacatcctg aaagatcggt tccttgtatg
     7981 agatcaggga gttttgaaat atgatgcgat gctttttcca gaagaggaca cgcctgactg
     8041 ccctcgctct ttgtctgatc ttgtctgtcg gatgcgggca aatgacggat aaagatcgta
     8101 tccgggtggc aaaagtgggg gagcggtaca ttacccgggg tgaccttttt aaaattatcc
     8161 gggaaatgcc ggacaatgtc cgccccatga tccgcaacag aagcgatctt gtacgcattc
     8221 tcaaccagca tattgacaac agcatcaaaa gaaaactggg catacagttt gccaaagaag
     8281 gaagagttgc tgtagacaga gaactggcgc gggaaatgtt tttccgggac agcggcgatg
     8341 aggaagaaag ccttcgcaac atgtggtcta tggaggttcc gccacccggt gtaattaccc
     8401 cgctgatgag tgtatacgga ctgacgcccg aactgctcca gttcaacaag gatcaggtcg
     8461 aagaaggaac cgaccgcatg atcatcaaac tgcagggtga gcaggcagtc gaagtacttg
     8521 ctgccgaggc gtttaaagac ggccgcataa cgcttgacga aggggaggtc gaacgggagt
     8581 acgggtttat gaaggaacag ttcattgttc ccgagtatct ggagttcaaa gggatccgtt
     8641 ttgtggatgc gcccgatgcg gccgcccgtg cttcggcgct tcgtgaacgc ctgcaggcgg
     8701 gggaaagttt tgatagcctt cttgaggaat atatggaaaa agagaaggaa gacaataccg
     8761 attacgtaat cacggcaggc attatcaatg acccgcagaa agtacatctt cgtgctttct
     8821 gggaagctgc gtcgggggct cagcccggcg atattgtcgg tccggtattt ttgcgcgcct
     8881 cccagcagcg taccgaagac tcttccggaa aaacagtgat tacggaacag ccgccctatt
     8941 atctcatctg taaagtgatc gccagccgtc ctcagtcatt gctgccttat gagcaggcgc
     9001 gcccgcaggt tatcgtgccg cttattaaag cgaagatgat gcacctgttg cgcgaagata
     9061 ataaagtgga gatatacgag gataaactgc cggatccttc acatatgcag gggcctgctg
     9121 ctgtttagtg gttgctggcg tccgactgcc tgatcttaag ttttcagatt gtatagcaca
     9181 aggagaaaat aaaatgcgtc gtttgattac catgtcatta ctgctgcttg tctgcgtcgg
     9241 atcggctcaa gcggatctga ttctgcccca tttcgagacc aatgtcgaaa tctacagact
     9301 cttcggtgaa gagcatcccg gaccctacaa acatccggcg tctattacgc agttggaaaa
     9361 cggggattat tatgtcgcct attacggcgg tgaaaatgaa tattctgcag aaacagcggt
     9421 gtacggatcc cgctgcaagc agggaaaaaa gaaatggtcg gctccgaaaa tcattgccaa
     9481 aacacccttt cagggcgtgg ggaaccccgt ggtctggcag gcgccggatg gcgttgtatg
     9541 gctgttctac aacaatcagt acggcgatac ctggtctaat gcaagggtga aagcaaaaat
     9601 ttccctggac ggcgcaaaaa gctggtctga ttcattcatg ctctgcatgg aggaagggtc
     9661 catggtgcgg gggcagccca tcgttctgga tacaggggaa tatctgctgc ccctttacaa
     9721 tgaaaaaggg gacgaccggg aattcacaga ttccagttcc gtgagttatt ttctgcgcta
     9781 caatccaaaa acgcaggaat ggacggaatc caaaaataga atccgctccc ctgagggtaa
     9841 tttgcaggct caggtcgtgc agatcagtgc gaccgacctc ttctgcttca tgcggcgcgg
     9901 cggcggatac ggccccacag aagacggctg gatgctgcgt tcagattcaa aagacggtgg
     9961 atttagctgg actgatgccg tgcgtactga ctttcccaac cccaactccg ctgtggaact
    10021 gattaaactg aagaatggtc acctgctcct ggtttacaat gacagcatgt ccgaccgtac
    10081 gccgcttacg ctggcggtca gcacggataa cggggaaacc tggcctttcc gacggatgat
    10141 tgccggaggg gacaatgact ttgcttatcc ctatgccatc cagggttcgg atgataaaat
    10201 ttatctgatt tatacaacca atagccgcac ttccatcatg cttgccgtat ttgatgaaag
    10261 agcagttacg gaatatgagc cttgaaaaaa ctggagaaga ggctcatttt ctgttgtttt
    10321 cttcactaaa agaggctttt gtgtgccgtt gctgatttac ttgctttttt gaaaaaatct
    10381 gttatactgt ttatagtatt ttctttatac atattgttcc aaccaaaaaa ggaatgctgg
    10441 catgaaacga aaaggattta cgttgattga gctgctggtc gttattgcta ttatcggtat
    10501 tctggcagcg atactcctgc cggcgctggc acgcgcccgt gaagcggcac gccgctccag
    10561 ctgcgccaac aacctgaagc aattcggttt gtccttcaag atgtatgcca atgaagcacc
    10621 gggtgaaaaa tacccgcctg tccagcatca gacctattgc tctccctgct ttggtgtact
    10681 gctgacacct ctttgtgtgg cagtgtatcc ggaatacatt tctgacccga acatttatgt
    10741 atgtccctcc agtgcttccc ataaaataca ggatatgtac tacgataacg ggacaacaat
    10801 cctccagttt atcggccagg acggcgacac ttcctacaac gactggtaca aggcaaactt
    10861 ttcctactcc tatttcggat ttgcctatga cctttgcgac agcgtccctg ccaatctcgt
    10921 agatattgat ccggcgctga tctccatact tcagattacc agcccgggtt ttacagtgcc
    10981 tgccaatacg aaggcaccgg cacagctgca gagacagtgg ctgaagattt tccaggaaaa
    11041 caatatggga gccatgccct ataaccgccg cggtccctgg cctccctttg aagaagatac
    11101 aaaaggcgtg tcgcctgtgg gcaatggcgg aagcgatgtg gtttaccgtc tccgtgaagg
    11161 tatagaacgc ttcctcatca cggatatcaa caatccggcg gcaagcaata tggcacagag
    11221 cgatgtagcc atcatgtggg atcttgtctc ttccaaggtg gatcggttca accatatccc
    11281 gggcggcgcc aatgtgcttt atatggatgg tcatgtttcc ttcctgaaat atcccagtga
    11341 aaaagcgccg gttaccaagg aagtcgctct cgtttcctcc atcttctgat tgtatttttc
    11401 ccggctgatt tttccggctt cgggctcctg tcggatcccg gagccggtac tatttttcag
    11461 cagccgaaag ggacttaaat gccgggggct tgtaaaaaca agaaaacgat ttcgtcaaaa
    11521 gaaggatgaa agatccggaa tatgcctgag caggacgatc agtttaaagg aatagtggaa
    11581 gggaacaggc gtacccggct ttcctgctgc gggttcagcg atcgcttcga cccttttcgc
    11641 atgagcgcgc ttcctcatgt cgccgcattc tatcaggatg ttttttcgga attgacaaaa
    11701 ggggctccat acaaccgcat tctggatgtc ggatgcggca cgggtttgta ttttgaatgg
    11761 ctgtgtgcgt tgacgaagcg gcttgacggt atcgaacctt cgcaggaaat gcttgctgta
    11821 gcccggaaat tcgtccttgc ccgggaactg cagggtgttt atctgcagga aggaacggca
    11881 gaatcacttc cttttgatga cgagactttc gatgccgttg ttgtgctgga tgcgcttcat
    11941 cacacaggag accgggaagg ggcgatagag gaaatacggc gtgttcttaa accggggggg
    12001 ctcctgcttg ttctggaacc taatatccgc agcccgctca tcgccgtgat gcattttttt
    12061 catcgtgaag agcgcgcggc gctgagggca aataagcctc gccggctgcg cgcctttctt
    12121 gcttcccgtt tcagtgagcg aagctggcgc ggcgcctgcc agattgttgc ggagctccgg
    12181 gggattcggg gactgctggc aaaaatatat cttctttttg tccggcatag cctttccgag
    12241 acctggtatc cccgtcagat ctggctggga atgaaaccgc cccttagcaa gccattggcg
    12301 tcacgccgga aaaagaaacg gaaccggtaa actgctttgt gtacgacatc ctttttcgac
    12361 agatgaattc aaggtttctg tctgccgagc agacgcccga tattgcccgg cgttttttcc
    12421 cattgcgtgg ccgcttcata gtcgggtgat ttccgttcca atgcccggaa ttcttttgag
    12481 tggatgctgt ttctgccgct tgccgtcttc tggcgtggaa aaatggaatg cagcatttca
    12541 tgatagataa catgccggat aaaaaagaga ggtacaaatt tctgatcgag ccggggattg
    12601 atgcgtatca gatgacaaca ggaacgatac gaaccgagtc tgatggaacg ggctttccct
    12661 tttgtatggc ggctccaggt aatcgtcgct gtgacctcgt tattgaaaga agactgatta
    12721 acctgtttaa agatctcacc cagattaaag aatcgcccct gatgaattat cttgtagcgg
    12781 tgtgttgccc cctgattttg ccgggcaatg atttcgtact ttctgatgtg ttcctgcaga
    12841 aaagagcaag acctttttga attggggttg agcagccact tcgctatatg attaatcacg
    12901 tcttctgatg cacaaagaaa catagagtgc atatgaacca gcaccttctt tttttcttcg
    12961 attatgcgaa aggcgagcat aagttgactg cattcggtaa tagacaaatc gatctgcagc
    13021 ccggtttggt gttcaagtct ggcttttatc tcggtggaag ggcaggcatg caactccggc
    13081 gggcaaagaa aagtgggatc ttcctgcccc cagtcaaatt ctaactgctg aggtcgtccg
    13141 ctgttattca tgttcactcg cttccgtact ttctccgggg tcaccttctt cagcatccgc
    13201 ttctgtttct tcagggggtt cggatgctgc tccttcttcc agcccggtct cctctgccgg
    13261 tacagcctca ggctcaccgt aaattaattc ctgaaagtaa gggttgtcgc gcagcgcact
    13321 gaagcagggg tcgcttgcag catccaggcg ggcatcggag ctgagtgtga tggcgaaggc
    13381 aagatagtcc agcgcctctt catcaagtcc ccgtgcacaa tacaggcagg caaggttata
    13441 cggtggcttg gagttctccg gagcgttttc catggcatag cggtaatgct cctctgcttc
    13501 ttcatacctg cccatcaccg ccagcaggtt ggcgtagttg ttgtgcgccg caacatcctg
    13561 tggttcgagt gtgatcgctg caatcgccat tgcctccgct tcttccagcc tgccgaggct
    13621 gcgtagcacc acgctcaagc tgttgtaggg acgaacccag tcgggttgca gtgcaatcgc
    13681 tgcagtcaga agttctgccg cttcttccag cctgcccgca tcggtgcgcg cgagtaatat
    13741 tcccagattc acataaggcc agctccagcc aggcgccaac cggattacct cgcgaaacca
    13801 cagttcagcg ttttccgtcg agttctcccg ctcgtactgc agcgcaagaa tataaagagc
    13861 ctccggagta ttcagccagc tcgcttcggc gctggcagcc accagccgca cgcacgcacg
    13921 ggtctcggga agctgcacgg tcgggtcatt ttccagcagt tccaacgtat aggaaaccat
    13981 gcgctttgtg taaatccgaa aaccttgatt gggcggatcc agataacgcc agccgacaac
    14041 aagcagcgca atgaaagcca ctgttgcaac gacaagccaa taggaatacc gcttcataaa
    14101 gatgcccctg tgaatagaaa gacggcactg ctccggataa ccacaaaaac gagccgcttt
    14161 aagtgtaccc tgaggacatg ctttctggca actgttttat tccgtttctc cggcttttgc
    14221 atgcctttcc aaagagaggc tatactgaat cgaacaacgg gatgccggtt tttcttcaga
    14281 agatccggtg caatacaagg agtcttagat gtctgacaga aaaaacacca gccgacgcgg
    14341 ttttttaaaa gcggccggcg gatcaggaat ggcggcgggc tacagtcttt ttattcgggc
    14401 agaccgggcg gagggcgccg atgcaccgct gccgccgggg gtggaaaagg ctgttgctgc
    14461 cgtgccgacc tctcccggtg cgccgcctcc ccatgagccg aacatggtgc aggtggattt
    14521 gcactgcgat tttcttgttg ccggcggggg aatggcaggt gtgtgtgctg cccttgctgc
    14581 tgcacggacg ggcgcacggg tggtacttgt gcagaatcgc tcacgcctgg gcggcaatgc
    14641 cagcagcgag gtgcgtatgc acatagtcgg tgccagctgt caccggagca gactgggctg
    14701 gcgggaaagc ggcattctgg aagagctgcg tctggaagat gcgaaacata atcctcagtg
    14761 gtcttttgaa ctttgggatc tgctgcttta cgacaaagtg gtcagtgagc cgaatattac
    14821 attgcttctg gacacctccg tattccgcgc agtggtatcg gacaatcgta tccgggaagt
    14881 ctgggcgcgc agcgatctta ccgaaacgct ttatcgtatt gaagcgcctc tttttgccga
    14941 ctgtaccggt gactgcaggc tgggggtgga ggcaggtgct gcctatgtag tagggcatga
    15001 atccagagat aaatataatg aagtccgcgc tccgaaaaca gcaggcacag aaacgctggg
    15061 cagcagcatt cttttcatgg caaaagacta cgggtacccc attcccttta cgccgccgcc
    15121 ctgggcgcgc aaagtgcagg cggagcagct gcgtttgcgc aaaatcagat cctgggaata
    15181 tggatactgg tggattgagt ggggcggtga agatgatgcc atccgcaata atgaagccat
    15241 tcgcaaggaa ctgctgtcca ttgtgctcgg tgtctgggac tacatcaaaa attcaggaga
    15301 ccatcccgac agtgccaatt ggggcatgga ttggatagga atgattccgg gaaaacgtgc
    15361 gagccgtcgt cttgtcgggc cgcatgtgct tacggaacag gacctgctcg gaatgaacgg
    15421 ttctttcgag gatgctgtcg ccgtaggtgg ctggcctttt gatgatcatc cgccgggcgg
    15481 ctttgatgca gttgatctgg ttccgagcga ttcgcagaat atcaaaaatg tctatggtat
    15541 tccgctgcgc gccctttatt ccgtgaatat cgcgaatctt tttatggccg gacgcaatat
    15601 cagtgcttca cacgttgcct ttacttctac ccgggttatg ggtacctgtg cggttgaggg
    15661 tcaggcaatc ggcaccgccg ccgccttctg tgccgcccgg aacatgacgc ctcaggcgct
    15721 ttatgaagac aaggagcttc tgaaacagta tcagcagcag ctgcttcggg atgaccaaac
    15781 cattcccggg cttgccaatg aagatgctgc cgatttggct cgccgtgctt cctatgtccg
    15841 tgcctctcat acggtggaag gcagccgccc ggagaatata attaacggtc tggtgcgtga
    15901 tatgggcgaa gagtggaaca accggtgggg cgctgaactg caggcaggaa ccgcctggat
    15961 cgaacttggc tgggaaagtc ctcacactat ttctctgata cagctgaccc atgactccgg
    16021 tttcagtcga gagctgacgc tgtccgggga gcagagtgtg cgcacgcctc agaagcgctc
    16081 ggcacaaccg gaaacttcca gagattaccg cctgctctac agggaaacag ggggcggtga
    16141 atggaaagag cttgttcagg ttgaagggaa ttatcagcga ctgcgccgcc atgcgttctc
    16201 gccggtccag gcacaggcgg tgcgcctgga ggtgactgcc accaacggct gtgatatcgt
    16261 gcggctctat gaaatgcgct gctacgcctg acgcggatta agacgatgag acgacacgat
    16321 cagattttac tgaagcaggg actgcaacat cctgccgggg tcgccctcgc caagtgagag
    16381 aatctgtctg ttgctatagc catgtcgccg gagtggctcg ataatgtctg ccgtcgcatc
    16441 gggcacataa aaacacagaa aagagccggt acccgacgct tcccaccagg gaagccaggc
    16501 aggaacgagt gctgcctctc ctggctggag cgaagcggtg ccgaattggt cttccagaac
    16561 cagttgctgc tgtagcgcca gaatgattct gaaagagctt ccggtcatgg tgaaacgtgt
    16621 tttgtgatac agggtaatcc gttgtgccgc aaaaaagcgg ctggcgcaga gatattcgct
    16681 tacgcaggtt tctccggtac agcggagagg tgccatccgg ccgctcgtgt cctcgccaaa
    16741 acgggcaact tgtaacccct tgtccacatg cagctcccgg ggaattcccc ggacatcgac
    16801 ccgaccccag tcgtaaagcc ggtaggtgat atcgctgttc tgctgaattt ccgcaagcag
    16861 gataccggca cctatggcat gtaccgcccc ggcggggaca aagatggaat cacccggaga
    16921 aacaggaaat tcgcggagca gttttccgca gccgccttca ggtagagagg tgcggaagct
    16981 gtcggcggtg acaccgtgtc tggtgcctga gattatccgg ctgtccggtg ccgcattcag
    17041 aatgtgccac atttctgtct tgccgccgtc aggctcgtgg agtgcctttg ccgccgcgtc
    17101 gtcaggatgc acctgcacgg aaagcatatc cccggcatcg atcagtttca ggagcagcgg
    17161 gaagcggtgg tttgccgccg ggtgtgctgc agtgcccagc agcctgtcgc cccattcttc
    17221 cataagcgtg cgcagcgtgt cgccttttcg ttcgcctgaa ataacccggc tttcgcattg
    17281 cggatgatca gcaatcagcc acgcttcgcc gatgcattga tctgccggca gcattttgcc
    17341 cagggctgca agtctgcttc cgccccataa tcggggggca tactgttcag aaaattgtag
    17401 aataccgaag ttcatgccgt ttattcctgc tccatagtgc cgcaatgatg agagcatggt
    17461 acagcaaaag gagggcatca gaaaactttg cagcggacgg accgggaaaa agaaatggtc
    17521 cgggcagtgc gtttgttcat gctttcagtt ctcctgtata gtgatgcctc tccgcaccgg
    17581 acatccccgg tggcaggttg atgagtcccg ctattccgga acgaatggcg ggtcttcatc
    17641 agtaatcaag gaatccggaa atgaatcagc atattatttg ggtattgtcc ctttgtttta
    17701 tcgcttccgt atcggacgga aaggaacttt ttgaagacgc gtccctccgc cacggctttg
    17761 aattgtccgc tgtggcatcc acgaaaaagc cgctcactgt atcccaaatt tttgtgaccc
    17821 atccttcctt taaaggaccc cgatggcggc ttgcccaatg ggggacccgt tatagtctgg
    17881 tcacggcgga ggaagaaaaa ctgccggacg gtacccgcag tctgtcaaac gccggaaaaa
    17941 cagtcctgct tcatccaggc gggttggcgg gcgaaggcgt gacgcttcgt gtgaacggcg
    18001 gtgcggaata tcctgaccgg ctgcggcgct ttggagagcc ctggcctcat ttgctggtgg
    18061 agcagcgttt gcgcgctgtt gcctgttctg actggcaatc gctcacattt catctggagt
    18121 tttttgttga gcagtgtatt ccggtgcggg acacagctca ggacccgtcc cttcatacgg
    18181 cacaggtagg tgtatttttt acagttcata acttgaataa gaaaagtgct gatttcagag
    18241 acatgatatg gttcgggctg cccgtgtatg atgtgcgttt tggcatttcg ccggggcatc
    18301 aggcgttgga catcggcaaa aatgacgcaa caggtaaatt tatctgcaca ttgtcgggcg
    18361 atcgttttta ttcggagccg gttcttccgg ggaaatggca taccctcagt gctgatctgc
    18421 ttcccttgct gcaggatgcg ctcgatgctt cgaaggagca tgggcacctg acgcactcgg
    18481 agttgaagga ccttcagttc acgtctttca atctgggctg ggaagtgccc ggcagttatg
    18541 attgcgctat ccgtttgcgg cgtctgagcc tgaagggcga agcaagcgac gaagtgcctg
    18601 gaaggagacg ctaaattatg tcaaagacac tctgtattca cctgatagaa ggagaggagg
    18661 gatgccgctt tgctgtggag cgtaaggcgg tagccgtaat tgtcgacgct ttgcgggcaa
    18721 gtgcgactgc cgctttgctg cttgaaaaag ggaccgataa gatttgtgcc gtccgtgagg
    18781 tggaagaagc actggaaatg aagcgtcact ggccggatgc ggtattgttt ggagaacggg
    18841 gcggacgccc gcctgccgga tttgactacg gaaacagccc ggaagaagcc gcctgtgccg
    18901 gaggacggtc tgtaattttt actacaacaa cgggtgtaag ccggctgatc agtgccagcg
    18961 gtgcttttgc ggcagtgatg gcaaccacag tcaacggtgc cgcactccta tctttcctcc
    19021 agcagacgga tgcagccgag gcggtgatca ttcctgccgg tctggcgggc gatccggggt
    19081 ttgatgccca ggaagactgg acagcggccg tttggctggc gggcctatgc ctcgaacatc
    19141 cggcacccgg cgcgtttttt tctctgggcg aaggtgcgga agcgttccgc cggtatcagg
    19201 aacgtatcag caaagaaggg ctatccggtc tttttcacag cgcgccccat gctgcccggt
    19261 taagggcgat ttctatggaa aaggatattg atttctgcgc ccggacaaat atttgttctg
    19321 ccctgccaat agcctgcgga cgccggggta ccggtgttct gctccgtgcc tggcgccctg
    19381 ccggatgaac attttcagtg attcaccggc ggtgtctata aaataacaaa actgcgggaa
    19441 taaccccgga aaaattgccg ttatacttgc aacaacaaga aaggacttgc tatgtcagat
    19501 aacacttccc gtttttcagc agatatgact gtagccgatg caatgaagct gcacccccgt
    19561 gccgccgaag tgtttgccgc ttttcattta ggcggctgtg cgcattgtca tatcaacgcc
    19621 ttcgaaacaa ttgagcaggt ttgcggtgcc tacggcgtcg aagtcagtgt tctgctcgaa
    19681 gtgctcgagg ggctttttac caacgaggca acttccgcct cctgacagat ttcgtaattt
    19741 atgcccggta ttgaatgaat ccccggtggc tggaatccat cggggatttc ttttttatgc
    19801 atggaaaaac gggggtttcc caggaggttt attgttgaca gatagttgac aaatgtgcag
    19861 aatactgtta cacttatgta tatttataag tgccttgaca gcactggaat accttcgggt
    19921 gggctgtatc agcccgggga ggtaatgttt tttgatgcgg aattgcaacg caatacctga
    19981 tatcgtataa aggaaaaccc ttatgacagc gaaagcgttc gggtggaaga gcggtctttt
    20041 ttctcttgtc ctgcttacag tatgctgcag ccttcccgca ctggcaaagc cgacaattaa
    20101 gattaatggt cccaatcccc attactggga atgcagtgtt gcgtacgatg atcagggagc
    20161 gattgcaaca gatagtaacg gagtggatat aacccgcgat atagttgttg atgggcttta
    20221 caacctggac acatcgattc tcaatgccga gtacgacatt gtttataggg tggtggatag
    20281 tacgggcgca tcggcaacag caagccggcg cgtcataatt gtggacacaa tacccccgat
    20341 ttttgccctc gaaaaagatt tggacaatgt gacgggtgaa gtgccgctcc ggctgatccc
    20401 tgtctccttc gatccggata agaagcctgt tccgccgcta tggcttatct ggctgaaaca
    20461 gcagttcgga ccttttgacg atcaggaggg agaagaggaa catccgaaac cggatgagtt
    20521 tgaacttact ctggcagaca ttgcaaattg gcccgaatgg gcgcagagaa tggcaatggt
    20581 ctggtatttc ttccctatag ggagaggtgt agtttacgaa attcctgatt ggggcgccaa
    20641 cccgatggag tggcggtgca gcggcaaaac ctgggaagac ccgggggtga aagtctttga
    20701 tgcctgcgat attgctgtgc cgctggatga agtatatgtt ttcatggtgg aagtgtatat
    20761 tgaggataat tatgtctggg aggaggagct ctggaacggg aaatatacgg acttaaatcc
    20821 taatcaactc aagtctccgg gaagcaaacg caatctggca ggatatcgcc tctattacta
    20881 tgctcccgac acttccagaa atatcggagt tgccgttcgt gatgtactcc cccggtatgg
    20941 cggatggctg acgctggcgg gcgaccctaa tttttccgtg aactgcggcg acgattccat
    21001 atggcccaca attttacaat tggagaaatt ggatcgttat tatgactcct gtgaagggga
    21061 tgcgtcttac aagatcagca aaagtggcgt agtaaactgg aacgtgcctg gcacctacca
    21121 ccttgtctac aaagcaggca atcacatcgc aaagcggacg attcaggtga atgatgccat
    21181 gccggtcatc acgcttcttg acaagaatca agcaccggtg agcagcccgg aagattcggc
    21241 ggtgacactt aactggtgtg agtacatcaa gcttgaccca tcggcggaag gtgctgtcat
    21301 tgtggatgaa tacggattac agcgctggtg gcctttgcgt cccgatgaag gtttctatgc
    21361 gacagacgga tgcgatgacg gttatgtgat ttcatcgcag acgcaggcgt acggttccgg
    21421 tgagatttcc aaacagctgc ttaagctcgc tgccagcggc attgccgaag cgcataccgt
    21481 ctatgaacgt atctggtatg aggcaaggga tccccgtaac aatcgggcat acaaagaacg
    21541 ccccgccata attgtttatg tgccgccgat tctttctttc aaagaaagcg gcggcagctc
    21601 ctggataccg tcgcaggtct atttagaagc caataagttt cagtgtggca ggtcgattac
    21661 cctttcccag ggaaaatttg atcttctggg ctgtaatgtc ctgaatgtac ctgcttatgt
    21721 cacggataca gggggactgg atctggaaaa tccggaagtc ggtatttact cggtaacgta
    21781 cagaagtttt cctgacgatc cggcaagagg gcttgctttg acagtgcaag tagaagtggt
    21841 agacaatcag gaacctggag taatactgga cactttcgga acattgcaga acaacgagtg
    21901 gtttattccg tgctttgata cgacaacaca ggatgcatat acgcttaccg atggctgcag
    21961 tccggcgact gatctgaaac tgcagaaata cgtctggcgt ctgttgggtg atctggcgac
    22021 ggatgcggtg cctgacgtgg ttcgtgataa gaagggcaga ccggtcgact acggcgctaa
    22081 tgacctcttc aaaaaagacc cgggctctta tatcattgtc tatgttgtca cggatgctga
    22141 aggcaatacc aatttacccc ttgcatccga cggcttgccg aatgtgtttc attccaacgg
    22201 cggcgttaat gtgtttgcca gtcccggtgt cctttccaag cgctttgaag aatctgtgcg
    22261 catagttcgc acgcaagcgg caacgcgtct tgttccggat ctgccgatgg atgacgaaga
    22321 cggattttat ctcgttagct gtacggaaga tataaatact cttctgaaca acagtgtgat
    22381 caataactgt tttgccgacc gggttgaccg ttttgtctgg aaattccaga atgacggcag
    22441 tatcaaacgg gaacgaaccg gagagccttc ggaatatgtg ccggaacagc tgaaagggcg
    22501 tcttggcgat tatgtggcgg ttctggtttc ctatttggat cgtcagacgc agcctttgct
    22561 taacgagaaa gtcttcgccg atattctgaa tgaaaaagga tttcttattg atcctgatgc
    22621 accgtacctg atgcgtttcc ggattcagga caccgatcct cctgtgatga cgcttaaagg
    22681 tgacgccgtg gtgacactgc tttgcggtac aaaatatgtg gagttgggct gcagcgccgt
    22741 ggatgaatgc gaaggtgttc tcgacagtat caagcaggag cctctgccaa aagcaataga
    22801 tacttcaaag ccgggcagct acacgattac ctattccgcc gaagataaat cgggcaacac
    22861 tgcttcagtg actcgcacag tgaatattgt tgatttgact gccgatgatg ttgaaatcgt
    22921 gatgctgggc gaagcggaaa ttgcattgga atgcggatcg cactttacgg atcccggcgt
    22981 tgaagtccgc tacaaagaga agaagctgaa cgtctactcc cgcagtgtgg attacttcgg
    23041 agtcatcggt gagactacgg gtacggtgcg ggttaattat tatgccacga ctcctgccgg
    23101 tacggaactg ctggtcgggc agcgtaccgt gattgtccgg gatacaacag cgcccgttat
    23161 tcaacttaat ggcgagtcag cgttgaagct gacgcaaggc agtgtctatt ttgagcgtgg
    23221 tgccagggtc tatgactcct gcagcgggca ggtcgatctc cgcgtagatg gtcaggtaga
    23281 caccggaaag ataggtgttt acacaataac ctatggagct tcggatgctt ccggcaacac
    23341 ggcaacggct gtccgtacgg tcactgttga aaaagagaag gcgaatgccg aaggcgagac
    23401 gcctgaagtt gtcgaaggtg aaattgaagg cgagtcccct gttgaaggcg aagaagatga
    23461 tgattgcgta ggctgcttcg ggcgcacagg atgctgcggt gcccggaaaa tgcccaaaaa
    23521 ccttctgggt gaatatttgc tgctgggtgt cagcatgctg gcactggccc ttttcagacg
    23581 gcgtggataa atcagaacag gcaccct
//
LOCUS       DAOPWB010000040        23531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2152_length_23531_cov_5.089879, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000040 DAOPWB010000000
VERSION     DAOPWB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 23531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23531
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_23531_cov_5.089879"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKN92_05490"
     CDS             <1..768
                     /locus_tag="PKN92_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48279.1"
                     /translation="EGEPVEGEPVEGEPVEGEPVEGEPDPCGPDLVAPELTVLHSEDA
                     VYNEEEGLFIYTVDCGTDVSAFGPVSVEAVDNCTEEPVIETLIYKVLGDDPENPEELA
                     VELVQNLGFDESNPWTNSPGYYFVLIKASDLAANTAENLYAVFVESNCGIVTRVVENY
                     TGVVTLLIAPPAGTTAWGLEDYAPDGLTFVSVDGVNGVIHPSGTKVTFWNNGESAAVV
                     TYRLSGPAGQHYFHGGVSIDGLPEVPIAGHQFILLAE"
     gene            930..1463
                     /locus_tag="PKN92_05495"
     CDS             930..1463
                     /locus_tag="PKN92_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48280.1"
                     /translation="MKGTLRFGTMVCLVSIFALSGLASAAAVEDGQDVWYLFKAGEKG
                     DYTITLTDSFAGATLAVFGGTAVGTEAGRKTAVPESHAKYTKPVELSVDKGATKLIRV
                     AGERIGTTDTFKNGSFKIALSQVKEKGGCGCGGKKLLLDGDAPFSLDALKNALGEWLL
                     VGLSALVLASWSVLRRD"
     gene            1612..3465
                     /locus_tag="PKN92_05500"
     CDS             1612..3465
                     /locus_tag="PKN92_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNZ48281.1"
                     /translation="MPRERVAGRFSRNGRQIVKEASFPRFFSAAFAVVSSLFLLFPFA
                     LSAGVQVEIIPMEQPDLQKVLESYSPMLWPGEGTPETETLLKGYAHRHEERLKDALHA
                     AGYYDAVLEMSVQWGDETSRVIYRVAPGNLYHFGSILLEMATPREEMDLFALETVLSE
                     STDLKKGSPALADSVLTGEKQLLRMLGNGGFPFARITDRKIRIDRENRTLHVTFVFDS
                     GQHLLFGDLRVEGLDSIRLDAVERELPWMRGAPFQISLLEEGQAKLQKTGLFSIVRLH
                     PAPEAEAVNGHIPIVAEVIERRHRTIHAGLQYRSDEGIGAGFEWEHRNFLEMSRRLRV
                     QGQITEMEQALGAVYDIQQYRRSDQNLTLHTRIRQLQPERYQSRRIEAGAWLERIISP
                     TLVYGLGPSLRMGRVQSTNKSDSYFLALFPFEVRYDQRDNPLDSHSGFQLTNRLTPFW
                     DVTNKNVFFAKNEMNFSYFIPFGEEQKTVLATRLRLGSLGGAASGMIPVDERFFAGGG
                     GSVRGYAYQNIGPLDSKGKPMGGRSLTDWSIELRRKLSEKIGVVAFVDGGMSYKTPFP
                     DFGTAPRWGAGIGLRYSTPLGPLRLDVAVPVNRRKDLDSSVQLYMSIGQAF"
     gene            3465..8192
                     /locus_tag="PKN92_05505"
     CDS             3465..8192
                     /locus_tag="PKN92_05505"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNZ48282.1"
                     /translation="MMEPKHKGRRRLGLLLFVPPLFGVLFCIFIFVSATGTAVLLRGV
                     ASGVSHFTDWDIAFEGVEGALRTSVTIKRILVSDDVGPCFLLENIHAAPRLSSFMQGY
                     ATLEQVDIERLTVLRRPVPKKKWRIPRVPAFLSRIRIDRLALACLQFPENTSAAEHPL
                     TLSGRIAPVSGRDLPEISLDVKSLQNNAVTGSISFSYVGAAPRLLVHFEDSAFAPDMI
                     GVAAPLKLEIRGDGPRDSWQASFLVEAQDTVLANGTLQLGEADLVTAVSGRGEVFLEE
                     IHRFASGAEILGNHIEGRIQCSLDTRGELVAEAMALRSEKLLVSGKSRLSLPDRTGTA
                     QFDLAYTPEETAASCRMALRAESDGKAVRYEIEGTGASGEFLGGEGSLALQDDYALTG
                     SLALHPGRFSAISLPFAPEEKMTSAYALRYSPEETQLTVEYISVEGAGASASFSGRLD
                     LEEDSFEWRGSGSVSESPWLQNLHGFSAEGAWEISLHAASREGGGEAELSVAGDGLRW
                     KDWAGGALTGTLHLNCDSWRHPAETPWHGKASLQCDSIVHYHEAASGEGEALMPDGAA
                     LTLELAGLGAKEIRIEDLRLQAAEAALMGQGRILPEEARAEAAFTLDSADGGKSLAPF
                     VSLPSFALKSSIEGNLSWDPLSLAYMIRGTVQQTASTPPSLSSWLEDKGLSFELAGQA
                     DPEKVEMKSGKAAHAAGSLSFEGSYGLRDGSFLFTAEGEDIALKTVGKLLDQDLAGKA
                     GVKASLSGTLAGAKGQLILTGKDVQRAPLPPLALQLKIQGEGPVRMPSLGWEGTLAAP
                     EAKLHFTGKGSVEAEHLSVERFDLALVDNRGSFRAVLPFDLKKAELHGSLTAGDLSAF
                     TAIAGMPVAGAGEGAIDFADNRTQSTLDFRDVQAGSFRASGLSLRVEAERLDRMPSGQ
                     VQLKIEQPVVGKAALDRVQAVLSGTLESAQLRLQINEMPDKKTEVLPLIALIAESDVS
                     LQQRTARLHRLDLAAERAFFTLKEASRLQWSDSRIVLNPVVLTSGERQIRLEGNAGQD
                     ELIGSVKIAALPLSLLEGAGLPGLSGTLQGEASLGGTPEHPELKSSFAVKELGLKDGS
                     TAGLPPASAKVELQLMEGYLHLRALAEMVPGMTVKTQARCPLPLRFKPWHLSLDKGGS
                     LDGQIHFDVDFKPLLKEMALIPHGLEGKLSGDFSIGGSLAAPLLTGAARFTEGQYTNI
                     TTGTSLRDFQFQLKAQDGTLLLQDCTARTGQQGMLKVDGHVLLDFEKHFPLALNAVLD
                     QAQVVHLAYARAVASGPLQLQGDLQGMLLSGTLEITPVEATMPDQLMLREPPRLEVIE
                     MGQEPAAAKSASLLENLTSRIRLDVNCSMPRKIYVRAPILDSEWKGVFHVGGTLSNPL
                     AEGRISALRGTLDFLNQRFQLSDSAVLLTGKSIFEPTMDLRAVVETREISATVRLEGE
                     MSTMVMKLESSPSLPQDEILSHILFGKDLARISPVQALQLARVAAMFNRDLGGLRFFT
                     GQLSISGIDRIDLRTGEKIDDAAVGLGKYFTDSVYVEVEQGTTSDSGRISVEVELSPQ
                     LSIKGDVDARDRSGVGLFWRKDY"
     gene            8240..9256
                     /locus_tag="PKN92_05510"
     CDS             8240..9256
                     /locus_tag="PKN92_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ48283.1"
                     /translation="MTGKERILNLIAGAPVDRVPVMPITMMFAADHAGIPYGRYVTDY
                     RALVDAQLKTAEDYGFDYVSCISDPAREAADCGAAVVFYENQPPAIQEETALLADKSS
                     LASLRMPLPEEGRRMSDRVNAVALFKEKGGDDFLIEGWVEGPCAEGADLRGINNLMLD
                     FIEDPAFVEALFEFALTLGLSFARAQLEAGADSIGVGDAAASLIGPHLYESLVLPYEK
                     RLVAGIQEMGGKVRLHICGDTSQLVGAIGTLGCDIVDLDWMSPLKAARVSMGDQQVLA
                     GNGDPVRIFRNGNEETVRSYLCSCLADGSPRYILAAGCELVRDTPEAHVRLFRQISEE
                     QAGR"
     gene            9287..10735
                     /locus_tag="PKN92_05515"
     CDS             9287..10735
                     /locus_tag="PKN92_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48284.1"
                     /translation="MSHTDCQGKELSACCPKAEISLGTYIVFPAVAVLLGWGLRGYIG
                     GGPFAALIPGAFLSLSLSLLLRHDTAMACQTALFCTVGLAFGGEMTYGQTLGLATAPE
                     TFWWGMLGVTLKGGIWGLLGGALLGAGLVLRRYETKRLFLACLLIPVASFIGWKIIND
                     PQLIYFSDPTNPREESWAGLLFATVVFLAVLYGSADKKAKQMPLRFALWGLVGGAIGF
                     GGGTLFLVYGPSLPVPQAWSNWWKAMEFFFGFMLGVFFGGCAWYYRGELAEEKAFVRE
                     KDRGLRDLLWFVPVIIFFFTLFPILDTASTEGNAWYLRDLMHFIYTFVFLGGLCLILG
                     LRSSPMTWQIVATLTVFHAVLDFNEWAPAAGEPNFSIIARALILVLSIVGTGAAVLFL
                     QKRRHFVPHLFQLLLWACYGIACLKVFAVKKWLLTDADAGYRFINCWLDHYAIVPVFV
                     IFTVSAVITALYICRVYDESTGEVSLSVQEHG"
     gene            complement(10740..11390)
                     /locus_tag="PKN92_05520"
     CDS             complement(10740..11390)
                     /locus_tag="PKN92_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ48285.1"
                     /translation="MKTPFDFTADNYDLWYDTPEGQAAFQGEWLCIRDLMKGSPSGWL
                     EAGTGTGRFAHALAIGTGIDLSSRMLRIGRQKGLRLCQGRAEALPFPSAAFQGVLFAL
                     ALCFMDDAKQALQEAARVLMPGGQLLIGMIVPESRWGGLWKASAHKGHPLYSRAHFRS
                     ITETVALAEQSGFLFTDARSALRWEPGTVPQEPVQALKGIHPAAGFAALSFERIPG"
     gene            11797..12303
                     /gene="infC"
                     /locus_tag="PKN92_05525"
     CDS             11797..12303
                     /gene="infC"
                     /locus_tag="PKN92_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNZ48286.1"
                     /translation="MRVNEEIRARQVRVIDEEGAQLGIMSPRDAIREAAHRGFDLVEV
                     APDAVPPVCRIMDYGKYRYEQKRRAREARKNQHTVSLKEIKYRPKIDKHDFEFKTNHV
                     REFLKEGNKVRVTIMFRGREMAHQEFGREIIQRVIEATQDLCVGDNTAGLAKMEGRNM
                     SIVLTPAK"
     gene            12432..12629
                     /gene="rpmI"
                     /locus_tag="PKN92_05530"
     CDS             12432..12629
                     /gene="rpmI"
                     /locus_tag="PKN92_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006693988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNZ48287.1"
                     /translation="MPKLKTNKSAAKRFSRTKNGSFKRHHAYARHLMSSKTPKRRRNL
                     RQSALVSSEEVPRIKRLLPYA"
     gene            12712..13074
                     /gene="rplT"
                     /locus_tag="PKN92_05535"
     CDS             12712..13074
                     /gene="rplT"
                     /locus_tag="PKN92_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNZ48288.1"
                     /translation="MPRATNNPASRQRRKKVLKLAAGYRGARHKLYKTAQQAVDHSGQ
                     YAYRDRRNRKREFRRLWIARINAGARANGITYNRLIEGLHKADISLDRKMLAELAVNN
                     AEAFAALVEKAKATLAAV"
     gene            13077..13826
                     /locus_tag="PKN92_05540"
     CDS             13077..13826
                     /locus_tag="PKN92_05540"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0016860 - intramolecular oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HNZ48289.1"
                     /translation="MRIVPAVDIRGGLCVNLVQGDFDQETVYSQDPVDQAQAWWHALL
                     EAGQKDDSLIHMVDLDGARTGACCILPQLKMLARRGIPYEVGGGIRSLETIEQILEAG
                     AARVILGTAAYRDRELLAESCRRWPGKIAVGIDARAGQLVLSGWLENTDADALVFARE
                     VAEAGASRIIYTDILSDGMLSGPNQEMTGAVARTAAIPVTLSGGVASLDDIHNARALA
                     ALGVDEVIVGRALYVGRFTVEQAVRAARERP"
     gene            13823..>14236
                     /locus_tag="PKN92_05545"
     CDS             13823..>14236
                     /locus_tag="PKN92_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNZ48290.1"
                     /translation="MNIGFERITIIGVGLLGASLGLALKRRKSGARITGVGRRAESLE
                     KALQCGAVDEISLDPLSASAQADAVIIATPVQQVIPLLDLLAPHLSPNALVMDVASVK
                     QEICDHARRIFPVPRRFVGCHPMAGGEKFGPENGTA"
     assembly_gap    14237..14246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14247..14674
                     /locus_tag="PKN92_05550"
     CDS             <14247..14674
                     /locus_tag="PKN92_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNZ48291.1"
                     /translation="VCLMEDSPDLSEEAQQAARAFWEFVGTRVVTLNAARHDALLART
                     SHLPHVAAAALARIVYDAGADSTLIGRGFLDVTRIAASRAEIWRDISLSNRGALLGAI
                     QELQQSLDAFEAALTKGDAAAVEDFFEKAAEARGKVTAS"
     gene            14671..15318
                     /gene="tmk"
                     /locus_tag="PKN92_05555"
     CDS             14671..15318
                     /gene="tmk"
                     /locus_tag="PKN92_05555"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNZ48292.1"
                     /translation="MTGLFITLEGGEGSGKSTQIGLLSQWLRQQGYEVLATREPGGTP
                     LAEEIRTLLMVPRSEKMSSLAELFLYQAARAQHVQEVIRPALLRGAVVLCDRFTDSTR
                     AYQGAGRTLPDETVVQLNQLAAQDIQPDITFILDLPEEAGMQRARDRGSDDRMMTEAL
                     AFHRNIRAAFQRLAKEEPDRITMVDGTLSREAVLAVLQERILPRLPARPMPEKRS"
     gene            15384..16514
                     /gene="holB"
                     /locus_tag="PKN92_05560"
     CDS             15384..16514
                     /gene="holB"
                     /locus_tag="PKN92_05560"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNZ48293.1"
                     /translation="MSFSQIRDQEIALRLLQGMCRRERIPNGLLFWGPDGVGKSLAAL
                     EFAKAVNCEARNGDACGQCLSCRKITNRNHGDILYIAPEGKGRLINVKAIESMTEMVI
                     YRPFEGGTRVVLIYDADRMHEAAQNKFLKTLEEPPSKTVFVLITTSPRRLLPTILSRC
                     HRIRFRALQEATVVQILREKEQCSEARALAIAPLAQGSMTRALDLLHSDRREAVLDII
                     KRLVSREDPLVVSELFTAHLQEKEKQITAELKAEWSGKPEEGGESPEMEKEGLEALVA
                     GRIRREVQEYLRLFDSWYRDEMMQAVSAGEALLFNRDQMAHLARDISLDGQSVKLAAI
                     NEAARYIERNMQSQRVFRDLFFELARPVTGTALRPPGRQAGG"
     gene            16563..17426
                     /gene="ricT"
                     /locus_tag="PKN92_05565"
     CDS             16563..17426
                     /gene="ricT"
                     /locus_tag="PKN92_05565"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNZ48294.1"
                     /translation="MNIVHVRLRKSRRVMVLSSGDLVLQRDDPLIVNTEQGLEWATCI
                     LPPEPCVDTAHLPAELRVVRKATVSDEHALEALEAEERAAWEVCYKRIEKRNLPMRLV
                     AAEYTFDKHKITFYFTAENRVDFRELVRDLAKDLHSRIELRHIQVRDRSKLVGGLGCC
                     GRELCCASWLNHFVPISMRMAKCQNLSLNPGKISGQCGRLLCCLQYENEVYESGDIQC
                     CQIAALQEKGDEASASALEEEAPSRNNIVVAAPEQHWEKHTVSSSNQDEKKKGKNARR
                     KKKKKPGKNQP"
     gene            17423..18184
                     /locus_tag="PKN92_05570"
     CDS             17423..18184
                     /locus_tag="PKN92_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNZ48295.1"
                     /translation="MKGVDTHCHLQMAPFDEDREAALDRALQVLKWLVVVGYGLDGSE
                     SALALCRPGVYATAGFHPYLASGYDDAGEARLKELAQKKGIVALGEMGLDYFNEFSPR
                     PEQRVCFERQLALAAELQLPVIIHCREADEDTEAILKDYISDLPECIMHCFGGTASFA
                     ERCVGMGCYVSFAGNVTFPKAQTLREAALVVPLERLLAETDAPYLAPQSFRGKRCEPA
                     HVMTTLTFLAQLKGLPPEQLIGQVVKNGEQAYRLC"
     gene            complement(18211..20106)
                     /gene="mgtE"
                     /locus_tag="PKN92_05575"
     CDS             complement(18211..20106)
                     /gene="mgtE"
                     /locus_tag="PKN92_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNZ48296.1"
                     /translation="MSINAQTAAPSSSLIQQAIEEGRVIELPLCNKEEALRILAESLE
                     SARDGDAAVPSIIDSILHYESQSTAYLGYGIACPHARGGNEGEMICAVGWSPDGIEYG
                     NTDGWPVHLLLMYYVPYPARNKYLTELSSLARAIAADEDHHELVNLEDLDEVKERLNT
                     WIAAMEGRIDPEDGRKAASRVASSLLSQVLIPDILEMLEDRRLRDLRIFLSAQPAPEI
                     AELITALDSSDQLLVFRLLPRSLADEVFSLIDYPSQTGLLKNMAQDETRQVLAALSSD
                     DQTALFEELPANVTQRLLTLLSDADRKQVLSQLSYPKDSVGRLMSSGYVSAQENWTIA
                     KTMEHIRAAGSDSETVMTIYVIDDSGALVGELRLRQLILADPALRVSVLMDKNYVALH
                     SIQDREEAVLIFKKYDVYALPVIDSEGVLLGIVTNDDILDVAEEEATEDFHKAGAIRP
                     LSVGYLKTPLIMLYRSRLPWLIALVFVNIFSGAGIAHFEELLGVYMALVFFLPLLIDS
                     GGNAGAQSATLVIRSMALGELSLKDFARVFWREALVASALGLSMSVAVFAVAWWRSGT
                     LIAVVAALAMVSIVILSSMLGMLLPFVLRRFKVDPAVASGPLVTSLADILGVVIYLSI
                     ASIILST"
     gene            20362..22671
                     /locus_tag="PKN92_05580"
     CDS             20362..22671
                     /locus_tag="PKN92_05580"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNZ48297.1"
                     /translation="MHLLFALAFVSLSSFSAMPAVDGVYMLAPWDKASTVSIPESGRY
                     TLWLAADPEAGGGLLLKDKKYTVGKGEKDAKGRSAFWRNAGTAALEAGVLKVEWSGGI
                     YLLVLSRDAEYTPERGLSDVRVLPFNRKPEDARLTKERHTDTVFIMPHFDSVEAWEKA
                     AAAIRLRIRLGSGLFPAPEKTPLNAHVFDEKEYDGFSVSKVYFEPVPGFLATGNLYRP
                     LGEGPFPAVVSPHGHWTAGRLENTDLGSIAGRCITLARMGIVTLSYDMIGYVDSIQLP
                     HRWGTEAEKLWGIHPFAMQLWTSLRAVDFMEALPYVDPERLGCTGASGGGTQTFALTA
                     LEPRIRVSAPVNMISSTMQGGCLCENAPLLRLTNSNMEIGALAAPHPLLMVSTTGDWT
                     RETPRVEYPAVRSIYALYGAEDQVVNVHLDFPHNYNRQSREAMYRFFGAHLLGGDWHA
                     YEEPPFEMPQEEALRVFPGDRLPEGYLAGEALRAQLMNQAQERRAHHLEQALKAPDSF
                     AEEYHEALTALFAAELPGKNALSLERLEMEEREGYVLERWLIGRISEGDRVPLLLYRG
                     TGALPQESILLVHGAGKAAFADLQHGGPGSLIRRLLKEGKMVATLDAFLIGEYNGPER
                     AAKRVEKGKFSDPFLPAVASCRVQDILTAAAFMRSRFDTAGRIDLVGVEEGGLWCLFA
                     GAVDRSLHSVYVDLNRQNYSDDSLWESRFFVPGIRSFGDIPAACVAGGFSRFHFFNGS
                     LPPELAHSGISVEADDPSDETLTTWLVGK"
     gene            complement(22675..>23531)
                     /locus_tag="PKN92_05585"
     CDS             complement(22675..>23531)
                     /locus_tag="PKN92_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNZ48298.1"
                     /translation="RYMSGAYEPHSQAFIRYASAAAALLLYTAFRMPGALKEAFARWR
                     ILLSMSLLVLLMQFAWTVAIYHTTATKAQLVTTLQAPLVILLSFLVFREERAVIGSWR
                     YLLGTLSCIAGVLAIFIRRSGGGLSLTVDLALILLIFVSITWTIYAVWGRHTAKSLHP
                     IALFAVLSVFVSAGFLLSTLLLGDISCLATAGPRMNFIAFISGVICIAAAHCAFHHAQ
                     LHLGSAYCTSLHLTSPFITHIIALFFWTDEKLVLLQQLGGFLLIAGCLLVIRSRVTGS
                     AISEESGQ"
BASE COUNT         5105 a         6031 c         7030 g         5355 t
ORIGIN      
        1 gaaggtgaac ccgtagaagg cgagcccgta gaaggtgagc ccgtagaagg tgagcccgta
       61 gaaggtgaac ccgatccttg tggccctgac ctggttgccc ctgagcttac tgtcctccac
      121 agcgaagatg ctgtttataa cgaagaagag ggtctcttca tttacacagt ggactgcggc
      181 accgacgttt ccgctttcgg gcccgtatcg gtagaagctg ttgataattg cactgaagag
      241 ccggttatag agactctgat ttataaggtg cttggcgacg atccggaaaa tccggaagag
      301 ctggcagttg agctggttca gaatctcggc tttgatgagt caaatccctg gacaaacagc
      361 cccggctact atttcgttct catcaaagcg tcagaccttg ccgccaatac tgccgaaaat
      421 ctgtatgccg ttttcgtaga atccaattgc ggaatagtga cccgggtcgt tgagaattac
      481 accggggttg tgacactgct tattgcgcca cctgccggta caaccgcctg gggtctggaa
      541 gattatgctc ccgatgggct gacttttgtc tctgtggatg gcgtgaatgg cgttattcat
      601 cccagcggga caaaagtgac cttctggaac aatggtgaaa gtgctgccgt tgtgacctac
      661 aggctgagtg gtcccgcagg acagcactat ttccacggtg gcgtcagtat tgacggtctg
      721 ccggaagtgc ccatcgccgg tcaccagttc attttgcttg ccgagtaact caggaagaac
      781 tgcctgatcg cgggtctctg agaggcaccg tgatggacag tgtgttgtta acacagttga
      841 aaagagaagt aactgccggg cgtcaggagc gcctgcaccc ggcagtccca atacgccgca
      901 tcaggcggca ctcagaagga gaacttttca tgaagggtac attgagattc ggtacaatgg
      961 tgtgcctggt aagcattttt gcattatccg gattggcgtc cgcagcagcc gttgaagatg
     1021 gacaggacgt ctggtacctg tttaaagcgg gtgaaaaagg cgactataca attacgctga
     1081 cagattcttt tgccggagca acccttgccg ttttcggcgg cacggcagtc ggcacggaag
     1141 ccggccgtaa aaccgctgtg ccggagagtc atgccaagta cacaaagcct gttgaattaa
     1201 gtgtagacaa gggcgccaca aagctgattc gtgttgccgg tgaacgtatc ggaaccacgg
     1261 acactttcaa aaacggttcc ttcaaaattg cactcagcca ggttaaagaa aaaggcggat
     1321 gtggatgcgg cggcaagaag ctgctgctcg atggcgatgc cccgttctct ctggacgccc
     1381 ttaaaaatgc gcttggagaa tggctgcttg tcggtttgag cgcccttgta ttggcttcct
     1441 ggtccgtact gcgtcgcgac tgatccacct attgctgaac gaactgtatg cctgatggca
     1501 gcactgcttt tgctgtcatc aggctttttg ttttttgacg ttccggctga cttttttttc
     1561 aaggcacttc gcccgggctg agtgtcttcg ctgtccggag tgtggtatgc tatgccaagg
     1621 gaacgggtag cagggcgctt cagcagaaac ggaagacaga tcgtgaaaga agcatcattt
     1681 ccaagattct tttcagctgc ttttgcagtc gtctcttcgc tttttcttct gtttcccttt
     1741 gcgttgtcag ccggcgtgca ggttgaaatt attccgatgg agcagcctga tttacagaaa
     1801 gtgctggaaa gctattcgcc catgctttgg ccaggggaag gaactccaga aactgaaacg
     1861 ctgctgaagg gctatgcaca tcgtcacgaa gaacgcctga aagacgctct tcatgcagcc
     1921 ggatattatg atgctgttct tgaaatgagt gttcaatggg gtgatgaaac cagccgggtt
     1981 atttaccggg ttgctccggg aaatctttat cacttcgggt ctatcctttt ggaaatggcc
     2041 acgccccggg aagagatgga tctttttgcg ctggaaacgg tgttgagcga aagcacggat
     2101 ttgaaaaagg gctcgccggc actggcggac tccgtgctta ccggggaaaa gcaattgctt
     2161 cgcatgctcg gcaatggggg atttcccttt gcgcgcatca ccgatcggaa aatacgcata
     2221 gatcgtgaaa acaggacgct tcatgtaacg tttgtttttg acagcgggca gcatctgctt
     2281 tttggcgatc tgcgtgtgga aggcctggat agcatccgcc tcgatgcggt ggagcgggag
     2341 cttccctgga tgcgcggtgc gccgttccag atttctcttt tggaggaggg tcaggcgaag
     2401 ctgcagaaga caggactttt ttccatagtt cggctgcatc ctgcccctga agctgaagca
     2461 gtaaacggtc acattcccat tgtggccgag gtgatcgagc gtcggcaccg taccatccat
     2521 gccggacttc agtaccgctc cgatgaaggc atcggcgccg ggttcgaatg ggagcaccgg
     2581 aattttctgg agatgagccg ccgccttcgc gtgcagggac agatcaccga aatggagcag
     2641 gcgcttggtg cggtgtacga cattcagcaa taccgccggt ctgatcagaa tctcacgctt
     2701 catacccgaa tcagacaact gcagcccgaa cgatatcaga gcaggcgtat cgaagcaggc
     2761 gcatggcttg agcgcatcat atctccgacg ctggtctacg gtctggggcc ctctttacgg
     2821 atggggcgtg tccaatcaac caataaaagc gatagttatt ttctggcact gtttcctttt
     2881 gaagtgcgct acgaccaacg ggataatcct ctggacagcc acagcggatt tcagctgacc
     2941 aatcggctga cccctttttg ggatgtgaca aataagaacg tgttctttgc caaaaacgaa
     3001 atgaatttca gttattttat tcccttcggt gaagaacaaa agaccgttct tgcgacgcgg
     3061 ctgcggctgg gcagtctggg aggcgcggct tccggaatga tccccgtgga tgagcgtttt
     3121 tttgccggtg gcggcggaag cgtgcgtggg tacgcctacc agaatatcgg ccctctggac
     3181 agtaaaggaa agcccatggg cggtcgttcg ctcacagact ggtctataga actgcgcagg
     3241 aagctgtcgg aaaaaatagg agtggtcgct tttgttgacg gcgggatgtc gtataaaacg
     3301 ccttttcctg atttcgggac agcaccgcgc tggggcgcgg gcatcgggct tcgctacagt
     3361 acgccactgg ggccgcttcg gctggatgtg gcagtacctg taaaccgccg caaagatttg
     3421 gattcgagtg tacagctgta catgagtatc ggtcaggcct tttaatgatg gagccgaagc
     3481 acaaaggtcg caggcggctc gggctgttgt tatttgtccc gccccttttt ggtgttcttt
     3541 tttgtatttt tatttttgtg tctgcaacgg gcacagcggt gctcttgcgc ggggtggcgt
     3601 cgggcgtcag tcactttaca gactgggaca ttgcttttga gggcgttgaa ggtgcgcttc
     3661 gcacatcagt aacgattaaa cgcattttgg tcagtgatga cgtcggaccc tgttttcttc
     3721 tggaaaatat tcatgcagca ccccgcttgt catcgttcat gcaagggtat gcgacgctgg
     3781 agcaggtcga catagaaaga ctcactgtgc tgcgccgtcc ggttccaaag aaaaaatggc
     3841 gtatcccccg ggtcccggca ttcctgtccc gtatccgtat cgaccggctt gctctggcat
     3901 gtcttcagtt cccggaaaat acatcggctg ccgaacaccc tctcacgctg tccggtcgca
     3961 tcgcgcctgt ttccggaagg gaccttcctg aaatctcgct ggacgtgaaa agcctgcaga
     4021 ataatgcagt aacgggaagc atttcctttt cctatgttgg tgccgccccg cgtctgctgg
     4081 tgcattttga ggattctgct tttgcccctg acatgatcgg agttgcagca cccttaaagc
     4141 tggaaatacg cggtgacggc ccaagagaca gctggcaggc gtcttttctg gtggaggcgc
     4201 aggacacagt tctggctaac ggaacacttc agctcggtga agccgacctg gtaacagcgg
     4261 tctccggcag gggcgaggtt tttttagaag agattcaccg ttttgcttct ggagcggaga
     4321 tactcggaaa tcacattgaa ggtcgtattc agtgcagcct ggacacgcgg ggtgagcttg
     4381 tggcagaagc gatggccctc cgatcggaga aactgttggt ttccgggaag agcaggcttt
     4441 ctcttccaga ccggacgggc actgcccagt ttgatctggc atacacgcca gaggagacag
     4501 cggcatcatg ccggatggca ttgagagcgg aaagcgacgg gaaggctgtc cgctatgaaa
     4561 ttgaggggac aggcgcgtcg ggagagtttc tcggagggga aggcagcctg gcacttcagg
     4621 acgactatgc tctaaccggc agccttgcgc tgcacccggg acgcttttct gctatttcac
     4681 ttccttttgc accggaggaa aaaatgactt ccgcctatgc gctgcgttac agcccggaag
     4741 aaacgcagtt gactgtggaa tatatatcgg ttgaaggtgc cggtgcgtcc gcatcgttct
     4801 ccgggcggtt ggatcttgag gaagacagtt ttgaatggcg tggcagcggc agcgtctccg
     4861 agtctccctg gctgcagaac ttgcatggct tttcagcgga aggcgcctgg gagatatcgt
     4921 tgcatgccgc ctcccgtgag ggcggcggag aagccgagct ctctgttgca ggagacggac
     4981 tgcgctggaa ggactgggcg ggcggtgctc tgacagggac gctgcacctt aactgcgaca
     5041 gctggcgaca ccccgctgag acaccgtggc acgggaaagc atcgcttcag tgtgactcca
     5101 ttgtacatta tcacgaggct gcttccggag aaggggaagc ccttatgccg gacggcgctg
     5161 ctctcactct ggaacttgcg gggctgggtg cgaaagaaat ccgcattgag gatttgcgtt
     5221 tacaggcagc agaggcagct cttatggggc agggtcgtat tcttccggag gaggcaaggg
     5281 cagaagcagc cttcactctt gacagcgctg acggcggaaa gagtcttgct ccctttgtgt
     5341 cccttccctc ttttgcgctg aaaagttcca tcgaaggcaa cctctcctgg gatcccttgt
     5401 cacttgcata tatgatccgg ggaaccgtac agcagacagc gtcaacgccg ccttcgctgt
     5461 catcctggct tgaggacaaa ggtctttctt tcgaacttgc cgggcaggcg gatccggaaa
     5521 aagtggagat gaaatccggc aaagcggctc atgcagcggg cagtctttct tttgagggca
     5581 gttacgggct gagagacggc agtttccttt tcactgcgga aggggaagac atcgcgctaa
     5641 aaactgtcgg caagctgctt gatcaggatc ttgccgggaa ggctggagtg aaagcgtctc
     5701 tatccgggac acttgccggt gcgaaagggc agctgattct taccggaaaa gacgtgcagc
     5761 gggcccctct gccaccgctg gcactgcagc tgaaaattca gggcgagggg ccggttcgta
     5821 tgccgtcact gggttgggaa ggaacgctgg cagcgccgga agcgaagctg cactttacag
     5881 gaaaaggctc cgttgaagcg gagcatcttt ctgttgagcg ttttgatctt gcgcttgtcg
     5941 acaacagggg cagtttccgt gcggtgcttc cttttgatct gaaaaaagcg gaattgcacg
     6001 gcagcctgac ggcaggcgac ttgtccgcat ttacagcgat cgccggaatg ccagtagccg
     6061 gggcaggaga gggcgccatt gatttcgccg acaaccggac gcaaagcacg ctggacttcc
     6121 gggatgttca ggcaggcagc tttcgtgcct ccggtctgtc gctacgggta gaggcggaga
     6181 ggctggatcg gatgccttcg ggacaggttc agctgaaaat tgagcagcct gtagtcggga
     6241 aggcagctct ggatcgtgtg caggcagtgc tgtccggaac gctggaatca gcacagctgc
     6301 gcctgcagat caacgaaatg cccgacaaaa aaacggaggt attgccgctc attgctctta
     6361 ttgccgaaag cgatgtgtcg ctgcagcaac gcactgcacg cctgcaccgt ctggatctgg
     6421 cggcggaacg tgcgttcttt accttaaagg aggcgtcccg gctgcaatgg tcggattccc
     6481 gcatcgtact gaatccggta gtgctgactt ccggcgagag gcagattcga ctcgaaggca
     6541 atgccggtca ggatgaactt atcggctctg tgaagattgc agcgctgcct ttgtcccttc
     6601 tggaaggcgc cggccttccc ggactcagcg gaacgcttca gggcgaagcg tctctgggcg
     6661 gaacgccgga acatccggag ctgaaaagca gctttgccgt aaaagaactt ggtctgaaag
     6721 atggttccac tgccgggctg ccgcctgcct cggcgaaggt tgaacttcag ttgatggaag
     6781 gttatctgca tctccgcgcc ctggctgaaa tggtgccggg gatgacggta aagacacagg
     6841 cgcgctgtcc gcttcctctg cgtttcaagc cctggcatct gtcactggat aagggcggca
     6901 gtctggacgg acaaattcac tttgacgttg actttaagcc gctgctgaaa gagatggcgc
     6961 tgattcctca cggcttggag gggaaacttt ccggcgattt cagcattggc ggcagtctgg
     7021 cggctcccct gctcacgggt gcagcacggt ttacagaagg gcaatatacc aatattacaa
     7081 cgggcaccag tctgcgtgat tttcagtttc agctgaaagc acaggacggc acgctgctgc
     7141 ttcaggactg caccgcccgt acgggtcagc aggggatgtt aaaagtggac gggcacgtgc
     7201 tccttgattt tgagaagcat tttcctctgg cgttgaatgc cgtgcttgac caggctcagg
     7261 tcgtgcatct cgcttatgcc cgggctgttg cttcaggccc gctgcagctg cagggagatc
     7321 tgcagggaat gctgctttcg ggtacgcttg agataacgcc tgtggaggcg accatgcctg
     7381 atcagctgat gcttcgtgag ccgccccgtc tggaagtgat cgaaatgggg caggagcccg
     7441 cagcagcgaa gagtgcttct ttgcttgaga atctgacttc ccgcatccgt ctggatgtca
     7501 attgctccat gccccggaag atctatgtgc gcgccccgat tctggattcg gaatggaaag
     7561 gcgtgttcca tgtaggcggc actctgtcca atccgctggc agaaggtcgt atatcggcat
     7621 tgcggggcac ccttgacttt ttgaatcagc ggtttcagct tagcgacagt gcggtgctcc
     7681 tgacagggaa aagtatattt gagcccacaa tggatctgcg tgcggtggta gagacccgcg
     7741 agatttccgc cacggttcgg ctggaagggg aaatgagcac gatggtgatg aaactggaat
     7801 caagcccttc tcttcctcag gatgagattc tttcgcacat tcttttcggc aaagaccttg
     7861 cgcgcatctc cccggttcag gcgcttcagc ttgcccgggt tgccgccatg ttcaaccggg
     7921 atctgggtgg ccttcgtttc ttcaccggtc aactttctat ctccggaata gaccgcattg
     7981 acctgaggac aggtgaaaag attgatgatg ccgctgtcgg tctgggaaaa tatttcaccg
     8041 actcggttta tgtggaagtg gagcagggta ccacgtccga cagcggtcgg atctccgttg
     8101 aagtggagct ttccccgcag ctgtccataa agggcgacgt ggatgcccgt gaccggagcg
     8161 gtgtgggact tttctggcga aaagactatt gaacaatcat ctgccacggc ggatgaagaa
     8221 aagagaaagg ttcagcggca tgacaggaaa agaacgaatc ttgaatttga tcgccggagc
     8281 gcctgttgac cgggtgccag taatgcccat caccatgatg ttcgccgctg atcatgccgg
     8341 catcccttac gggcgttatg tgacggatta tcgcgctctt gtggatgcgc agctgaaaac
     8401 cgcagaagac tacggcttcg attatgtttc ctgtatatcc gatcctgccc gggaagcggc
     8461 agactgcggc gcggcagttg tgttttatga aaaccagccg cccgccattc aggaagagac
     8521 agcgctgctt gccgataaaa gcagccttgc ctcgctccgg atgccgcttc cggaagaagg
     8581 cagacgcatg tctgatcgtg tcaatgcggt ggctcttttc aaggaaaaag ggggtgatga
     8641 ctttctgatc gaggggtggg ttgaagggcc ttgtgcggaa ggagccgatc tgcggggcat
     8701 caataatctt atgcttgatt ttattgagga tcccgctttt gtggaggcgc tcttcgaatt
     8761 tgcgctgacc ctgggactgt cttttgccag ggctcaattg gaagcaggcg ctgattctat
     8821 cggcgtgggc gatgccgccg cttctctcat cggcccgcat ctttatgaaa gcctggtgct
     8881 tccttatgaa aaaaggctgg tggcgggcat tcaggaaatg ggcggcaaag tgcgtcttca
     8941 tatctgcgga gacacaagtc agctggtggg tgccataggc acgcttggct gtgatattgt
     9001 ggatctggac tggatgtctc cgctgaaggc tgcgcgggtt tctatgggag atcagcaagt
     9061 gctggcgggc aacggtgatc cggtacggat atttcgtaat ggaaacgaag aaacggtgcg
     9121 cagctatctg tgctcatgcc tggctgacgg gtcgccgcgc tatattcttg cggcgggctg
     9181 tgaactggta cgggatacgc ccgaagcgca tgtacgactc ttccgccaga tttccgagga
     9241 acaggcagga cgctgaagct gccgaaaaat atacaggagt gttttcatgt ctcacacaga
     9301 ttgtcaggga aaagagcttt cggcgtgctg tccgaaggct gagatttctt tgggaaccta
     9361 tattgtattt ccggctgttg cggtactgct tggctgggga ctgcgcggat atataggcgg
     9421 cggtcctttt gcggcactga ttccgggtgc ttttctttcg ttgagcctca gcctgctttt
     9481 gcgtcacgat accgctatgg catgtcagac agcattgttc tgcacggtgg ggctagcctt
     9541 tggcggagaa atgacctatg ggcagacgtt gggcctggcg acggcgccgg aaactttctg
     9601 gtggggcatg ctgggcgtga cgctcaaagg cggtatctgg ggtcttctgg gcggcgcact
     9661 tttaggggca ggtctggtgc tgcggcgcta tgagacaaaa aggctgtttc ttgcctgcct
     9721 gctgataccg gttgcttctt ttatcggctg gaaaataatc aatgatccgc agctgattta
     9781 tttttctgat ccgacaaatc cgcgagagga aagctgggca gggcttctgt ttgccacggt
     9841 tgtttttctt gcggtgcttt acggcagcgc cgataaaaag gcaaaacaga tgcctttgcg
     9901 ctttgctctg tggggattgg tcggtggtgc gattggcttc ggcggcggca cgctttttct
     9961 tgtttacggt ccgtcactgc cggtgcctca ggcgtggagc aactggtgga aagccatgga
    10021 gtttttcttc ggctttatgc tgggtgtctt ttttggcggc tgcgcctggt attatcgcgg
    10081 cgagctggca gaagaaaagg catttgtacg ggagaaggac cgcgggcttc gtgatctact
    10141 ctggtttgtg ccggtgatta ttttcttttt cacccttttc ccgattctcg atacagcgag
    10201 cacggaggga aatgcctggt atctccggga tctcatgcat ttcatataca cttttgtttt
    10261 ccttggcgga ctctgcctga ttcttggtct tcgctcttcg cccatgacct ggcagattgt
    10321 ggcgaccttg actgtttttc atgcggtgct ggattttaac gaatgggcgc ctgcagcggg
    10381 cgaacccaat ttttcaatca tcgcccgagc ccttattctg gttctgagta ttgttggaac
    10441 aggagcggcg gtgctctttc tgcaaaagcg ccggcacttt gtgccgcatc tttttcaatt
    10501 gctgctctgg gcgtgctacg gcattgcctg tctgaaggtt tttgctgtga aaaaatggct
    10561 gcttaccgat gcggatgcgg ggtatcgctt cattaattgc tggctggatc attacgccat
    10621 tgtcccggtt tttgttatct tcacggtttc agctgttatt acggcgttat acatttgccg
    10681 tgtgtatgat gaaagcacgg gagaagtgtc gctttctgtt caggaacacg gatagctctt
    10741 cagcccggta ttcgctcaaa gctgagggcg gcaaagcccg ctgcgggatg gatgcccttc
    10801 agcgcctgaa ccggctcttg gggcacggtg ccgggttccc atcttagagc gcttcgtgca
    10861 tccgtaaaaa gaaagccgga ttgttctgca agcgcgactg tctcggttat gctccggaaa
    10921 tgcgcccggc tgtaaagagg gtgccctttg tgcgcggatg ctttccatag gccgccccag
    10981 cggctttccg gcacaatcat accgatcagc agctgcccgc ccggcatgag cacccgcgcc
    11041 gcctcctgaa gcgcttgttt tgcatcgtcc ataaaacaca atgcgagtgc aaaaagcact
    11101 ccctgaaaag cggcggaggg aaaaggcagc gcttctgcac gtccctggca gagccggagt
    11161 cctttttgac ggccaatacg cagcatccgg ctggagagat caataccggt gccgatcgca
    11221 agcgcatggg cgaaacgccc ggtacccgtc cctgcttcca gccagccaga cggactgcct
    11281 ttcattaaat ctctgatgca aagccattct ccctgaaaag cggcttgccc ttccggcgtg
    11341 tcataccaga gatcgtagtt gtctgcggta aagtcaaaag gcgttttcat ggctttttct
    11401 gcattcaggc ggctggtgtt tttttaaatt atatgcccct ttggtcctgc ggtcaactgc
    11461 cgggaaaaag gcaggatacc ggggatttcc gcattccgga ctgagcggca tttgacaaaa
    11521 gaggttactc tctgatacag tatggctacg gatcatagtt atccgtcagc agaagaagga
    11581 aaatattcct caccttgcgg cggcttcgag cctgccagcc tggttgcgca ccacagaaaa
    11641 tcatgattta gtatgtgtac gcgacgaagg caggtgtctt ctgtcgcgtt ttttattttt
    11701 ccggataaga ccggatcctt tgtgttatat gtcgttcggc ccttaagcgg cccataggag
    11761 aagactctca tcggcaagga aaaaaagcaa gaccaggtgc gggtgaacga agaaatccgc
    11821 gcccgtcagg tgcgggttat tgatgaagaa ggcgcccagc ttggaattat gagcccccgg
    11881 gatgcaatcc gcgaagcggc acaccgcggc tttgatctgg tggaggtggc accggatgca
    11941 gtgcctccag tctgccgtat catggactat gggaagtatc gctacgagca gaaacgccgt
    12001 gcccgggaag cccgcaaaaa tcagcatacg gtttctttaa aagaaatcaa ataccggccc
    12061 aagattgata agcatgactt tgagttcaag accaaccacg tccgtgaatt cctgaaagaa
    12121 gggaacaagg tgcgcgtgac catcatgttc cgcggccgcg aaatggcaca tcaggaattc
    12181 gggcgcgaga tcatccagcg ggtgattgaa gctacccagg atttgtgtgt gggcgacaat
    12241 acggcaggtc ttgccaagat ggaaggccgt aatatgtcca tcgttctgac cccggcaaaa
    12301 tagcgtctgt ctctgacaga gatacagcat cagacttaac aggcatcgtc tctcataaaa
    12361 gggacagatg ttttgtccgg tcttagtcac atgaagaaac actgagcatt gtactacagg
    12421 agattgcagt tatgcctaaa ctgaaaacga acaaaagcgc agccaagcgt ttcagccgta
    12481 cgaaaaacgg atctttcaaa cgtcaccacg cttatgcgcg ccaccttatg tcctccaaaa
    12541 cccccaaacg tcggagaaat cttcgtcagt cggctttggt ttcgtctgag gaagtcccgc
    12601 ggatcaaacg gcttcttccc tacgcctgat cgtcgggagg tattccgggt acaacacagg
    12661 gatcccggca gcggattcca ctgattcaga gagtaaacaa ggagatttat catgccccgt
    12721 gcaacaaata atccggcttc ccgccagcgc cgcaaaaaag tcctcaaact ggcagcaggc
    12781 tatcgcggag cccgccacaa actgtacaag acagcgcagc aggccgttga tcacagcggt
    12841 cagtacgcct atcgtgaccg acgcaaccgg aaaagagaat tccgccgcct gtggatcgca
    12901 cgcatcaacg cgggtgcccg tgccaacggc attacctaca atcgcctgat cgaggggctt
    12961 cacaaggctg atatcagtct ggaccggaaa atgcttgccg aactggcagt caacaatgcg
    13021 gaagcttttg ctgccctggt tgaaaaagcc aaagcaacat tggcggctgt ataatcatgc
    13081 gcattgttcc tgcggtggat atccgcggcg gattgtgtgt gaatcttgtt caaggtgatt
    13141 ttgatcagga aaccgtctat tctcaggatc ctgtcgatca ggcacaggcg tggtggcatg
    13201 cgctgcttga ggcgggtcag aaagacgatt ccctgattca tatggttgac cttgacggag
    13261 cacgtacagg cgcctgctgt atcctgccgc agctgaaaat gcttgcccgc cgcggtattc
    13321 cctatgaagt cgggggcggc atacgctctc tggaaaccat agaacagatt cttgaggcag
    13381 gcgcagcccg ggtaattctg ggtactgctg cctaccggga tcgtgaactg ctggcagaaa
    13441 gctgccgacg ctggcccggg aaaatagcag taggcatcga tgcccgcgct ggtcagctgg
    13501 tactcagcgg atggcttgaa aacactgatg cggatgcgct tgtctttgcc cgtgaagtgg
    13561 cggaagcggg cgcttcacgc attatctata cagatatcct cagcgacggc atgctctcgg
    13621 gacccaatca ggaaatgacg ggagccgtcg cccgtaccgc agcaattcct gtaacccttt
    13681 ccggaggggt cgcctctctg gatgatattc ataatgcccg tgcactggcg gctctcggcg
    13741 tggacgaagt gattgtgggc cgtgcccttt atgtcggtcg ttttactgtg gagcaggcgg
    13801 tgcgggcagc acgagaaagg ccatgaacat cggctttgag cggattacca ttatcggtgt
    13861 tggattattg ggagcctctc tggggctggc actgaaaaga cggaagtctg gtgcccggat
    13921 taccggagtg ggtagacggg cagaaagcct ggaaaaagcg ctgcagtgcg gtgctgtcga
    13981 tgaaatttcc ctcgatccgc tcagtgcgtc ggcacaggct gacgcggtta tcatcgctac
    14041 cccggttcag caggtcattc ccctgctgga tctgctcgct cctcatcttt ccccgaacgc
    14101 tcttgtcatg gatgtggcaa gtgtcaaaca ggaaatatgc gaccatgccc gacggatttt
    14161 tcccgtaccg cgtcgctttg tgggctgtca tcctatggcg ggcggtgaaa aattcgggcc
    14221 ggaaaacggt actgccnnnn nnnnnnccgt ctgccttatg gaagacagcc cggatctcag
    14281 tgaagaggca cagcaggcgg cccgggcatt ctgggaattt gtaggtaccc gcgtcgtcac
    14341 cctcaatgcg gcgcggcatg atgcgcttct tgcccgtacc agccatttgc cacatgtggc
    14401 ggcggcagct ctggcgcgca ttgtgtatga tgccggagca gacagcacac tgataggcag
    14461 agggtttctg gatgtgaccc gtatcgccgc ttcccgcgct gaaatctggc gcgacatcag
    14521 tctgtcaaac cggggtgcgc ttctcggagc gatccaggaa cttcaacaga gcctggatgc
    14581 gtttgaagct gctctgacaa agggtgatgc cgcggcggtg gaagactttt ttgagaaggc
    14641 ggctgaagcg cgcggcaagg tgactgcctc atgacaggac tttttattac actggaaggc
    14701 ggagaaggct caggaaagag cacacagatc gggctgctct ctcaatggct gcgacagcag
    14761 ggctatgaag ttcttgccac acgggaaccg ggcggcacgc cgctggcaga agagatacgc
    14821 acgctcctga tggttccccg aagcgaaaaa atgtcctctc tggctgaact gtttctgtat
    14881 caggcggcgc gggcacagca tgtgcaggag gtcatccgtc cggcactgct tcgcggagcg
    14941 gtggtgctct gcgaccgttt caccgacagc acccgggcct accagggcgc cggtcggacg
    15001 cttccggatg agacggttgt tcagctgaat caactggcgg ctcaggatat tcagcctgat
    15061 attacattta ttctggatct tcctgaagag gcggggatgc agcgtgcccg ggacagaggc
    15121 agtgatgacc gtatgatgac ggaagcgctg gcttttcacc ggaacattcg agccgctttt
    15181 caacgccttg cgaaggagga gcccgatcgt attacaatgg tagatggtac cctttcccgg
    15241 gaagcggtgc tggctgtgct gcaggaacgg attcttccgc ggctgcctgc ccgtccgatg
    15301 ccggagaaac gctcctgaac aggtgcgtcc gcttcctgat acgggatcgg acgtgctgca
    15361 gctatgaata acaggcaaag agaatgagct tttcacagat tcgggatcag gagattgccc
    15421 tgcgtcttct gcagggaatg tgccgccggg agcgcatccc caacggattg ctcttttggg
    15481 gaccggacgg cgtgggtaaa agtctggccg ctctcgaatt tgccaaggcg gtgaactgtg
    15541 aagcccggaa tggagatgcc tgcggtcaat gcctttcctg cagaaaaatc acgaacagaa
    15601 accatggcga tattctttat attgcgccgg agggcaaagg gcggctcatc aatgtgaaag
    15661 ccatagagtc catgaccgag atggtgatct accgcccttt tgaagggggt acccgtgttg
    15721 tcctgattta tgacgccgac cgcatgcatg aggcggcgca gaacaagttt ctgaaaacac
    15781 tggaagagcc gccgagtaaa actgtttttg tgctgatcac cacatctccc cgccgcctgc
    15841 tgcccaccat tctgtcacgg tgccaccgta tccgcttccg tgctcttcag gaagctacgg
    15901 tcgtgcagat tctccgggag aaggagcagt gctccgaggc ccgtgcgcta gccatcgctc
    15961 ctcttgctca gggcagtatg acccgtgcgc tggatcttct ccattccgac cggcgggaag
    16021 cggtgcttga cattattaaa cgcctcgttt cccgggaaga tccgctggtt gtcagcgagc
    16081 ttttcacagc tcaccttcag gaaaaagaaa aacagattac cgctgaactg aaagcggaat
    16141 ggtcgggcaa accggaggag ggcggcgagt ctccggaaat ggagaaagag ggactggaag
    16201 cactggtggc gggccggatt cgccgggaag tgcaggaata tttacgcctt tttgacagct
    16261 ggtatcgcga tgagatgatg caggctgtgt ccgctggtga ggcgctgctg ttcaaccggg
    16321 atcagatggc gcatcttgcc agggacattt ctttggacgg gcagtctgtc aagctggcgg
    16381 cgattaatga agccgcacgg tatatagaaa gaaacatgca gtcacagcgg gtgttccggg
    16441 acttattttt tgagcttgcc cggcccgtta ccggcactgc gcttcgccct ccgggcaggc
    16501 aggcgggtgg ctgatgcatc ggggcattcc ccgtacagta aaaaacacgc acaggtatgc
    16561 atatgaatat tgtacatgtc cgattacgca agtcccgccg ggttatggtt ctttccagcg
    16621 gtgatctggt gctgcagcgg gatgacccac ttattgtgaa caccgaacag gggctggaat
    16681 gggcgacttg tattctccct ccggagcctt gtgtggatac ggcgcacctg ccagccgagc
    16741 tgcgggttgt gcgcaaggca actgtttccg atgagcatgc gctggaagca ctggaagcag
    16801 aggagcgcgc cgcctgggaa gtatgttata aacggataga aaaacgaaac cttcccatgc
    16861 gtctggttgc ggcggaatac accttcgaca agcataaaat cactttttat tttaccgcag
    16921 aaaaccgggt cgatttccgt gagcttgtgc gggatctcgc caaagacctg cacagccgta
    16981 tcgaattacg ccatattcag gtgcgcgacc gctccaaact ggtcggcggc ctgggctgct
    17041 gcggcagaga attatgttgt gccagctggc tgaatcattt cgtgcctatt tccatgcgta
    17101 tggcgaaatg ccagaatctg tcgttgaacc cgggaaagat cagcggtcag tgcggtcgcc
    17161 tgctttgctg tctgcaatat gaaaatgagg tttatgaatc cggtgacatc cagtgctgcc
    17221 agatagccgc actgcaggaa aagggagatg aagcttcagc ctctgcactg gaggaggaag
    17281 cgccttccag gaacaatatc gtggttgccg caccggaaca gcattgggaa aaacatacgg
    17341 tctcctcttc caatcaggat gaaaagaaga aggggaaaaa tgcccggcgt aagaagaaga
    17401 aaaaaccggg aaaaaatcag ccatgaaagg agtggataca cactgccacc tgcagatggc
    17461 cccttttgat gaggataggg aagccgcact ggaccgggca ttgcaggtgc tgaaatggct
    17521 ggtggttgtc gggtatggcc ttgacggcag tgaaagcgct ctggcgctgt gccgtccggg
    17581 ggtctatgcc accgccggat tccatcctta tctggcttcg ggctatgacg atgccggaga
    17641 agcacgtctt aaagaactgg cacagaaaaa gggcattgtg gcgctggggg aaatgggtct
    17701 ggattatttt aatgaatttt cgccccggcc agagcagcgg gtctgttttg aacggcagct
    17761 ggcgcttgct gcagaactgc agctgcctgt gatcatccat tgccgtgaag ccgatgagga
    17821 cacagaggcg attctgaaag actacatttc agacctcccc gaatgcatta tgcattgttt
    17881 cggcggcacg gcctcctttg cggaaagatg tgtcggcatg ggctgctacg tcagttttgc
    17941 cggaaatgtg acctttccca aagcacagac gctgcgggaa gcggctctgg tggttccttt
    18001 ggaacggctt cttgcggaga ccgatgcccc ctatctcgcg ccgcagtctt ttcgggggaa
    18061 gcgctgtgag cctgcccatg tcatgacgac gttgacgttt ctggcgcagc tgaaggggct
    18121 tcccccggaa cagctgatcg gacaggtggt gaaaaacgga gaacaggcat acaggctgtg
    18181 ctgaggcgtc acctgccggc gtggtttgta tcatgtgctc aggataatcg aagcaatact
    18241 cagataaatt accacgccga gaatatccgc cagagaggta accagcggtc cgcttgccac
    18301 ggcggggtcg accttaaacc gacgcagcac gaagggaagc agcatgccca gcatgctgct
    18361 gaggatgaca atggagacca tggcgagggc ggcaacgacg gcgataagcg tccctgaacg
    18421 ccaccatgcc accgcaaaga cagccaccga catggacagc cccagggcgg aggcgaccaa
    18481 tgcctcgcgc cagaacacac gggcaaagtc tttcagcgat aactcgccca gtgccatact
    18541 tcgaatgacc agcgttgccg actgcgctcc ggcattgccg ccgctgtcta tcagaagagg
    18601 cagaaaaaac accagtgcca tatagactcc gagcagttct tcaaaatggg caatgcccgc
    18661 accggaaaaa atattgacaa atacgagcgc aatcagccac ggaagccggc tgcggtagag
    18721 catgatcagc ggtgtcttca gatagcccac cgacagagga cggatggcgc ctgccttgtg
    18781 gaagtcttcc gtcgcctctt cttctgccac gtccagaata tcgtcgttgg tgacgattcc
    18841 cagcagcacg ccttcggagt caatgacggg cagggcgtac acgtcgtact tcttaaaaat
    18901 cagcacagcc tcttcccggt cctgaatgga atggagcgcc acataatttt tgtccatcag
    18961 cacggaaacg cgcagtgccg gatcggcaag gatcagttgg cgcaggcgca attctccgac
    19021 cagcgcgccg ctgtcgtcga tgacataaat ggtcatgacc gtttccgagt cgctgcctgc
    19081 cgcccggata tgctccattg tttttgctat cgtccagttt tcctgggcgg aaacatagcc
    19141 gctggacatg aggcggccca cgctgtcttt gggataactc agctggctga gcacttgctt
    19201 gcggtccgca tcgctcagaa gggtcagcag gcgctgcgtg acgttggcgg gcaattcctc
    19261 aaaaagagct gtctggtcgt cggaagacag agcggcaaga acctggcgtg tttcgtcctg
    19321 cgccatgttt ttcagaagcc ctgtctgcga cggatagtcg atgagagaaa agacttcatc
    19381 agcgagactt ctgggcagca gtcgaaatac cagcagctga tcggaagagt ccagtgccgt
    19441 aatcagctcg gcgatttccg gcgccggctg ggcggacaga aaaatgcgaa ggtcgcgcaa
    19501 gcggcgatct tccagcattt ccagaatatc agggatcagt acctggctca aaagagagga
    19561 agccacacgg gacgccgctt ttcgaccatc ttcagggtct atccggcctt ccatggcggc
    19621 gatccaggta ttcaggcgct cttttacctc gtccagatct tccagattca ccagttcatg
    19681 gtggtcttcg tcggcggcaa tcgcacgggc aagagaggaa agctcggtca aatacttgtt
    19741 tcgggcggga taaggcacat agtacatcag aagcagatgc acgggccatc cgtccgtatt
    19801 accgtattcg atgccgtctg gactccaccc gactgcgcag atcatttcac cttcattgcc
    19861 gccccgggca tgagggcagg cgataccgta gcccaggtag gctgtggact gtgactcata
    19921 gtgcagaatg ctgtcgatga tgctggggac agcagcgtcc ccgtcccggg cgctttcaag
    19981 actttccgcc agaatcctga gagcctcctc tttgttgcag aggggaagct caatcacgcg
    20041 gccttcttcg attgcctgct ggatgaggct ggaagagggc gctgctgtct gagcgttaat
    20101 cgacatggaa gactctcctt gttccggagt gatgagggcg tggaaaaaag ttgtgtgtat
    20161 atattaacag aatcaggctt cttcagtcag ttaacggggc ttgcgggaaa atacggggcg
    20221 cccttccggt gcctctgaac tgcttcagcc ccgaagacca tggtctgccg ggaactgtgc
    20281 aggatttcct gcagacagcg gatttatctg cctgtgaatc ttcggttaca atgctgagat
    20341 caacctatag gagtctgcgt catgcatctg ctttttgccc ttgcttttgt gtccctgtct
    20401 tccttttccg caatgcccgc cgtcgatggt gtgtacatgc ttgcgccctg ggacaaggcg
    20461 tctacggtgt ccattcctga aagcggccgc tatacgcttt ggctggcggc agatccggaa
    20521 gcaggaggcg gtctgcttct caaagacaaa aaatacacgg tgggcaaagg ggagaaggac
    20581 gcaaaaggca ggagcgcatt ctggcgcaat gcaggcacgg cggcgctcga ggcgggagtg
    20641 ctcaaggtgg aatggtcagg cggcatttac ctgctcgtgc tcagcagaga tgccgaatac
    20701 acaccggagc gaggtctttc ggatgtgcgg gtactcccct tcaaccggaa acccgaagac
    20761 gcacgcctta cgaaagaacg gcataccgac acggtattta tcatgcctca ctttgattcc
    20821 gtggaagcgt gggaaaaagc ggctgcggca atccggttgc gcatccgtct gggcagcggg
    20881 ttgtttcccg caccggaaaa gacgccgctg aatgcccatg tgtttgatga aaaagaatac
    20941 gacggatttt ccgtatccaa agtgtatttt gagcccgttc cgggttttct ggctacaggc
    21001 aatctctacc gcccgctggg cgaaggacct tttccggctg tcgtcagtcc ccacggtcac
    21061 tggactgccg gcagactgga aaacaccgat ctgggttcca tagccggtcg ctgcattacg
    21121 ctggcgcgga tgggcattgt cacgctttct tacgacatga tcggctatgt ggacagtata
    21181 cagctgccac accgatgggg aactgaagca gaaaagctct ggggcattca cccctttgcc
    21241 atgcagttgt ggacaagtct gcgtgcagtg gatttcatgg aggcgcttcc ctatgttgat
    21301 ccggagcgac tgggctgtac gggcgcctcg ggcggcggga cacagacctt tgctctgaca
    21361 gcgcttgagc cgcgcattcg cgtttccgct cccgtcaata tgatctccag taccatgcag
    21421 ggcggatgcc tgtgtgaaaa tgcgccgctg ctgcgcctga ccaattccaa tatggaaatc
    21481 ggtgcgctgg cggcgccgca tccgcttctc atggtgtcaa cgaccggtga ctggacacgg
    21541 gaaacaccgc gggtggagta tccggctgtc cgcagcattt acgccctgta tggcgcggaa
    21601 gatcaggtag tcaatgtgca tcttgatttc ccgcataatt acaaccggca gagccgggag
    21661 gcgatgtatc gctttttcgg tgcccattta ctgggcggcg actggcatgc ctatgaagag
    21721 ccgccttttg aaatgccgca ggaagaggcg ttgcgtgtat ttcccggcga caggttgccc
    21781 gaaggatatc tggcgggcga ggcacttcgg gcgcagctga tgaaccaggc gcaagagcgc
    21841 agggcgcacc atctggaaca ggcattgaaa gcgcccgatt cttttgcaga ggagtatcat
    21901 gaggcactga ccgctttatt cgctgcggag ctgccgggaa aaaatgccct ctctctggaa
    21961 cgcctggaaa tggaagagcg ggaagggtat gtgctggagc gctggcttat cggcaggatc
    22021 agtgagggcg accgtgtgcc tctgcttctc taccgcggca cgggtgccct gccgcaggaa
    22081 agtattctgc tggttcatgg cgcagggaaa gcggcttttg ccgacctgca gcacggcggc
    22141 ccgggatctc tgatccgaag gctcctgaaa gaaggaaaaa tggttgcgac gctggacgct
    22201 tttctgatcg gcgaatacaa cggaccggag agagcggcga agcgtgtgga aaaaggaaag
    22261 ttttcagatc ccttcctgcc cgctgttgct tcctgtcggg ttcaggatat tctcacggcg
    22321 gcggctttta tgcgttcccg ctttgatacg gcgggccgta ttgatctggt cggcgtggag
    22381 gagggcggtt tgtggtgcct ctttgccggg gccgtggaca gaagcctgca ctctgtttat
    22441 gtggatctga accggcagaa ttacagcgat gacagtctgt gggagagccg tttctttgtg
    22501 ccgggcatac gcagttttgg cgatattccg gcggcgtgtg tggcaggcgg cttcagtcgc
    22561 ttccatttct ttaacggcag tctgcccccg gaactggcac actccggcat ttccgtcgaa
    22621 gcagatgatc cgtccgacga aacacttacg acatggcttg tcggtaaata aatatcactg
    22681 accggattct tcggagatcg cggagcccgt cacccgggac cggatcacga gaaggcagcc
    22741 ggcgatcagc agaaaaccgc ccagctgctg caaaagaacc agcttttcgt ctgtccagaa
    22801 aaagagcgca atgatatggg taatgaaagg cgaggtcaga tgcagtgatg tgcagtaggc
    22861 gctgccgaga tgcagctgtg cgtgatgaaa agcacaatga gcggcggcta tacagatcac
    22921 gcctgatata aaggcgatga aattcatgcg aggtcctgcg gttgccaggc agctgatatc
    22981 gcccagcagc agggtggaca gcagaaagcc tgcgctgaca aaaacgctca gcacagcaaa
    23041 gagggctatg ggatgcaggg attttgccgt atggcgcccc cataccgcat aaatggtcca
    23101 ggtgatgctg acaaaaatca gcagaatcag cgccagatct acagtaaggg aaagtccacc
    23161 gccactgcgc cgtatgaaga tggcgaggac acctgcgatg caggagagtg tgcccagcag
    23221 atagcgccag cttccgatta cagcgcgttc ttcccgaaaa acgaggaaac tcagcaaaat
    23281 gaccagcggt gcctgaagag tcgttaccag ctgcgctttg gttgccgtgg tgtgataaat
    23341 ggcgacagtc catgcaaatt gcatcagcag caccagcaga ctcatggaaa gcagaatgcg
    23401 ccaccgggcg aaggcttctt tcagtgcgcc gggcatgcgg aaagcggtat aaagaagaag
    23461 ggcggcagcg gcactggcgt aacggataaa ggcctggctg tgaggctcat aggcaccgct
    23521 catatagcga a
//
LOCUS       DAOPWB010000041        23459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2165_length_23459_cov_5.628910, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000041 DAOPWB010000000
VERSION     DAOPWB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 23459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23459
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2165_length_23459_cov_5.628910"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKN92_05590"
     CDS             complement(<1..365)
                     /locus_tag="PKN92_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNZ48299.1"
                     /translation="MESFSITGKQIISGVAVLSAGIFLGLLAGYGLFSSDEAQPELVS
                     LETLPPHSESAADPAPERIEQPGGSAIYSAEAKTEPVTAALPENKLCVAVEGAVRRPG
                     IYYFEEDSRVHDAIVAAGGL"
     gene            517..2775
                     /locus_tag="PKN92_05595"
     CDS             517..2775
                     /locus_tag="PKN92_05595"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HNZ48300.1"
                     /translation="MIQKLRLLLSVYLLIGTVFASSGIAAVTLSNSHMRLGFDESTGA
                     LIEACLAETGFNLVLPHANNSLWHLLRPDGTFITPEDACSFSFTKAPSENSVEFLWKG
                     FDDPRSRNLEVQVSVTLPENKQESHWNLALHDSDDFGFYQIAFPRMTSLAAQKNEALA
                     VPFWMGEVTSSARELLNPADAPALRRSWEYPGLLSLQCLTFYGDQGGLMLSTRDSTAI
                     RREFAVFGGERNALGIEISHPVSPASANYQLSGDVLLSLFTGDWFDAALLYRRERDAL
                     PYAEQSRVRTNQIPDWAEETGLWIWNRDASPGVLSPAAALQKEMAAPVSVFWHWWHGC
                     AYDAGFPEYLPPREGAESFKHALGAAQKEGIHAIVYMNQRLWGMDTTSWKEKDAQRYA
                     VKGADGTIRREIYNTFTQSPCASMCMGTSFWRNTYADIAEKVLLELGADGIYMDQACS
                     SLACYDQEHPHPPGGGPWWMQGFQQLASDIRQRCLPVKAPVLAGEGCGEAWLPHLDLM
                     LSLQVSLERYAAPGQWEPIPFFHAVYHDCARFFGNYCSLTRPPYDSLWPKESAPDRPL
                     ALLDKKFAAQFRLEQGRSFIWGQIPCIANFLPEQLTSRREELSFLKTLVHLRLKTLKY
                     LRDGIMLAPPDIGSPLIEIPTSRLSIYAGQHDAVQEYTRLVPSVLAAAWQASDGNVAV
                     ALVNIGDSTEEIELSLESSRYPLGEKGKVRRITADTEEEMGLWTQGQALHALTLEGSS
                     LAVVEYLCEMEP"
     gene            2808..5648
                     /locus_tag="PKN92_05600"
     CDS             2808..5648
                     /locus_tag="PKN92_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48301.1"
                     /translation="MEALFLSLLFAVSTVHLHENGTALTVETDYVRWDIGTKGETRSL
                     LDKSTEEELMSAENQKAFSSVLFEGKEWKATEISPSEKGYRILYGASGVEAEFRIQSY
                     PHYFSLSVHSLNNPSVEALTLLDLSLNLPAGEEEKTTACVLAANLRTHVPDLPGPASR
                     LRASAVKAFGIVGAQVNIILCPGAMLRPIMKEAVENSPELPRHKNQSVPPVGGPWALD
                     ASANKGSYLFDFGTLTEDTVDEWIDLVQQLGFRQIDFHTGTSLRFGDLTPNEKLFPEG
                     RKSVKSVIDRLHGAGIAAGLHTYAFFIAKNSPYVTPVPDPRLGKDEQFTLDKDLGETG
                     DALFVAESTEKVKTDTGFFARNSVTLQVENELIVFSGVTREPPFSFTGCVRGAHGTRA
                     SSHAAGTPVYKLKECFGLFLPDGNSTLLTEIAAVTADTFNECGFDMIYLDALDGSDIF
                     EGPENSWYYAPKFAFEIAQRLERPALFEMSTMSHHLWFIRARMGAWDHPARSHKRFID
                     LHCEVNRQNSTMFLPMNLGWWAVKNWQPGAASAWSEPTWPDDIEYLLCKALANGASLS
                     LMGVNPDNLDTMPAFERLLPLFKTYETARLSGMVAEEIKEQLRAPGAEFTLKTTDNES
                     FSFYPAATYKHTADFSDPFSLDWAVENPFDEQPVRFRIETLLSPASFDSQEAQVLLDY
                     TRPEALNVQKNNDGVSAEIAITESPLNENEHVAAFTLLSTRENARGSWAQIGAAFSPP
                     VNAGAKAGLGCFVYGDGSGALLNLQTLSAAHTAAGGIGDHYIPLDFTGWRYISVVEFE
                     SGAIPQWHWPYGDQYSIYREHVDFGALESFSFWFNNLPKDKTVHCKIGPVKALPLLEG
                     SLKESALIINGSTLSLPVTISSGCCLEYDPQKGAFLYNEKGEVLAQFSPSETILLKKG
                     TNSIRLTAANGAALPAARARITILTEGTVPLH"
     gene            complement(5651..7138)
                     /locus_tag="PKN92_05605"
     CDS             complement(5651..7138)
                     /locus_tag="PKN92_05605"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNZ48302.1"
                     /translation="MKIKMSLPIVCVCLFCMTACQHQTPMQAGNSFLSDLSLIKEGST
                     QRSSSCDADLENGNADARFIAPGGCLVIADLEGPGVIQHIWNTVAAVDRGYSRLLVLR
                     MYWDGESNPSVEVPLGDFFAMGHGLDHPMESFPVTVTSEGRARNCYWPMPFRKSARIT
                     VTNEGDGPVHAFYYYVDWTKVDRLPENTAYFHAMYRQEHPAVSGKHYLIADIEGRGHY
                     VGTVLNVRQHFPHWWGEGDDFWFIDGAEKPRLRGTGSEDYFCDAWGLREMSTPWYGAP
                     LMEGYESLARTSCYRWHIPDPVVFEKSLRLEIEHMGVTYNEDGTISSYFAERDDDFSS
                     VAFWYQTEPHKAYESLAPALERVYGGWAELIEAETKIDQCSVSHGAFAVQPEIGYNGG
                     QIFWTPQEPNGYLELEVDIDKDQVQELALMLTCARDYGTFQVLLDGIPLGAPVNLYGK
                     GINQKEFRFLVPACTPGKHILRFENRGKSPESIGYFFGLDAFMFM"
     gene            7333..9471
                     /locus_tag="PKN92_05610"
     CDS             7333..9471
                     /locus_tag="PKN92_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48303.1"
                     /translation="MSKVKLFIWILASVLLCAPVAFCDADAENNDDSLENTLDELAAL
                     GLDPDLIERDSFRKGTAPENFKERSGAEYKPSHNEEPNIPAQCWIETGYGTQNACLFC
                     HSNYLSKEKHGNSYPIADDQIFYSFPTPELNRILWRNTIFPQEISTRLEKEGIVIPDP
                     EDVSYMRKDNWKELYDRVRTNYPKRWLIPDDDEFLLFPALNPAHLYPEKQPDPTEGNK
                     HGYIDQEGFVRDEQNDYTGWRAVNFFPYGIFTPLTGSVSGIYIRLPEIFRTDAAIVDV
                     TLYKKNLDILERNIKNQKLTEKAYLGDASGVPVEKGFFPAGTEFAHPLHYVDLNADGE
                     SGERLDGVADQKGSDYEFPGTRSKRVKEIRYMYKWKSVTLEEITEDAHPENLVLGQEG
                     QGWIDNNMGWIIAGYIENRQGALRAQTTEELMQCLGCHGMTGNTVDSVWSFPRKLPGN
                     KGWRDMNYGEYNSQHPDRTALPDYRHGNTDSGEFGFFYKVVVGADLFGIMPDEVANEL
                     RAYAKNKGGSLQPEEDLDAILDDHGLIEMPRDQRKNLLTKRQQLMRSFVNDRAYLAKA
                     EGSEDPCIKGTLFYPSESTMKKNLHLYRQIVLDQSFNLGKDIFGSEEGHVPFSFRSDG
                     TVKNIKGEIIPAGEIITSRPHDKGGKGTTPTGIASVDQDGNLAGTDGNPVDMDASPEK
                     VGGHISTGGTFEPFYNPILSDIPLTEPGAK"
     gene            complement(9475..10347)
                     /locus_tag="PKN92_05615"
     CDS             complement(9475..10347)
                     /locus_tag="PKN92_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ48304.1"
                     /translation="MNSTSEMTRRNMLAALAAVSGAALVMPQGMAQEKAAVDVGRLKQ
                     SVSKWCFDKIPLVEFCQAVKPMGITGIDLLSEGDWETVAGEGLVCAMANGPGNIGKGW
                     NRIEHHEELIANAQRLIPKAGDLGVPNMVVFSGNRRGQDDETGLENCAKGLAKILPLA
                     EKAGVVLCMELLNSKRNHKDYQCDHTAWGARLAKKLDSPNFKLLYDIYHMQIMEGDVI
                     ATIEENIKYIGHIHTGGVPGRNEIDDTQELNYPRICSAIADTGFSGFLAHEFVPKAPD
                     PLESLRQAIAICRV"
     gene            10591..13017
                     /locus_tag="PKN92_05620"
     CDS             10591..13017
                     /locus_tag="PKN92_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48305.1"
                     /translation="MRHTIFICTIFIILFSSALFASENWHLCVLPEPASEEAVRLAVE
                     DLRLTADSLSIPLTMVEDAEKTEGNIIILSYAPQHPLIAKLIADGRWAPALPDNPEGF
                     EIATVPDAGRKIMLIAGASPVGNAYGLYWLRDRMRVYRTVPDINAVRQPQFSVRLGAA
                     WHRHGYGGSTKEQMRGALRNSINWVSGANILDLIPWHAEPEATENARHREETRELISY
                     AHSLGMKYFSFSNAYVYHPSILEEFGATLNPEDPRFWDAVQETYRRLFQALPELDGIE
                     LCNDDLSGFWGNYLPFDVMHDAPECDWSYVKRYNTFMQKVQEVVSGEFGKEHFYFTWG
                     LREHEIHCQPEVFKAVFRENIPTDHLYLMPKITRGDRWWHQPYNATFNLTPHKTVVLF
                     ETMNYYEGGASRLFPTFSGQYFQRGLQRFLAPEHSNVEGIALLSGPLREGWSTQDAYS
                     YVLYRLTWDVDEPMEDIALDFARIHFGEAAAAHMAQIYLASPAAYKYGLHIEPISYGQ
                     FNSFPHMRVNTFPAEGYPALDNGKEHLENLHKIYLRCEPWEEATLRAQYYGLEQAESM
                     LRHYDSARSAIEDDALSKEVGDRLHMTKNLIRTNIGYVTTMFAYFKYKENGIPENREA
                     LKKAIAQLAEAKNDFINTPGFGFQLFAVDILQRNAEALYENREEAIRHYNATPSREEL
                     DARIKKEQAGYRETLAKNKDKAVLFAKFELLCDGQDLVIIQGDQYHIKNLYWDGAHPR
                     KAEFLTPLPKKEVQVVIDDLESRPMHPFILEQPTADNDYTVKVYLDDQPGGNGWMNFD
                     LYYLDKESGM"
     gene            13060..14214
                     /locus_tag="PKN92_05625"
     CDS             13060..14214
                     /locus_tag="PKN92_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ48306.1"
                     /translation="MKICILNVLHDALDKRMYHKIARSLSAASHEIVSIAPPAAFAAG
                     RNQAESGETSVRFVFTPAVHSFFGRLLTVLRLIRLGLREKATLYIAPEPESWVAALLI
                     KAATGRKVAFDMHEYVPGEFAKFFPSFLRPPMEKTTCRIMRLMGRASDLIILTRRSFE
                     PLWSGIRTPRVVVINSNWLQPACTAVPDAVRKIAGERPTVLHQGIFGEIRGSLQLLEA
                     MKLVKEVLPEARCLVLGRYVYGDESAYRQKIKESGLEDTLLLLDEVPFEEVPAYISAV
                     QVGLILFQPGLRNHELAMPHKLFDYMREGVPVIAPDFAVEVAHIVKETQCGLLVDVTS
                     PDAIADAVIRLLTNPDEAQRFGRHGREAVESKYNWQKDSAALVDAIAALE"
     gene            14219..16078
                     /locus_tag="PKN92_05630"
     CDS             14219..16078
                     /locus_tag="PKN92_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNZ48307.1"
                     /translation="MGGLAGYTGCSDERLIHTMMHQQKCRGIRHARFLSRRDCTLSDF
                     EPETSDSGIRSVAAVLDGFISNRKQLQAELKAEGYPYECGSDTALLQHLWTARGISML
                     NRLEGMYAFALWDPQESCLYLVRDRTGSRPLYYHERKDTFFFASTIKGLLGYTGIRKT
                     LDLEGLDDYFAFLHPLPPRTFYKEIRQVPAGHYARRASGQLEVKRYWHPICQPKENCL
                     LEEWVVSTDTLLENIIDTYADAEASGGALLSGGLDSTVVLAYLSRHLSGVPSFTLGFT
                     EEEAHYDERRAARTTASLLKSACHEVFIHPDLPNNASFILEHFDEPFGNPSALLADIL
                     FREAAKSVSVVFTGDGGDEAFGGYPRYRGLLWAGLYRHMPFFLRRHVLHPLIHMLPER
                     AGGLHTLRRLRRFSEGSFLDPVDCYALWMEHQNSKERLALYNSNLTEILCGRDATEPV
                     RQLMRQCDSPDIITQALYADISLFLPANVLCYGDRLSHAHGVEMRAPLCEGALFTHLL
                     QAPSFLKVQHFADKQLLRMILHRQLPERKISRKKRGLNPPMSIWLNRWLEKPGAWEHF
                     LQETTAVDLFEPGRVWELLTEHRSGLRDHSWRLWPLYLFARWFNTPDPVLPTY"
     gene            16175..17233
                     /gene="trpS"
                     /locus_tag="PKN92_05635"
     CDS             16175..17233
                     /gene="trpS"
                     /locus_tag="PKN92_05635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNZ48308.1"
                     /translation="MSTTKEVILSGIRPTGRLHYGNYFGAIAHFLRLQERDANCYYFI
                     ADYHALTTITDRQEIYRSTLDMLRTYVACGLDPERSIIYRQSDLPSTAELALLLGMVT
                     NIGHLERGTTYKDKLGRLQDDPRSEGNPLSYGLLGYPVLMAADILIVRADLVPVGDDQ
                     RQHLEMACDIATKFNHRFGETLHIPKPLGRDALRLPGLDGSAKMGKSENNTLDLLDDP
                     ATVLRKIKAVPTTTDPLPLETESNAPEIVLPKMPAGVAVLYRLLSLLAPEEAYLDFLE
                     QYRQGGKFYGSLKKEVARFVSEFNAPIIEKYNDPAHNDAFIEDFLRENAKRVTPVALE
                     TVEACREAMGIGHSLFRA"
     gene            17247..18284
                     /locus_tag="PKN92_05640"
     CDS             17247..18284
                     /locus_tag="PKN92_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48309.1"
                     /translation="MDVIRIGFIGLGGICRQRHVPGLKRIAGIEFRAVCNSSRASSEK
                     AAAEFGIPKIYEQWEDLVQADDIDLVLIGAWPYLHHDASIAALKAGKHVFCQARMARN
                     AQEAQRMYTAAQTAKTLTAGLCPVPFGLRYDRTMTRLLQEGTLGSIRLVNVHSLSPLW
                     IDPAAPVNWRKNHHLSGLNMQTLGMYIEVIHRWFGLTTRASAETFLYTPVRKNTDGTD
                     FKIEIPDQILARTVVGTDLPVTYEISGVSALARETIDIYGDRAALHYDVASDSLYLCT
                     QNERVPVTPLPEEEYDLDNWRVEEDFINAIRQGLPYHPDFKDGLNYMQVIQAIYDSAK
                     NSSAAIAIQNL"
     gene            18302..18961
                     /gene="eda"
                     /locus_tag="PKN92_05645"
     CDS             18302..18961
                     /gene="eda"
                     /locus_tag="PKN92_05645"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNZ48310.1"
                     /translation="MDKTAIINYLIKHGIVAVVRADSGENLVPIIEAVMEGGVRCIEI
                     TMTTPGALECLALASEKLSGADVSLGVGSVLDGETARLAILAGAQYIASPIASAEVIN
                     MAHRYGKPCMPGAYTPTEIYTAWILGGDIIKVFPAGCGGLEYIKAVRAPLPQIPLIPT
                     GGVDLSNLADFIKAGVALIGAGGNLVSKKLIEAKDYQGLTENARRYSEALTQAREQLH
                     A"
     gene            18954..19661
                     /locus_tag="PKN92_05650"
     CDS             18954..19661
                     /locus_tag="PKN92_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNZ48311.1"
                     /translation="MPDMQSSFSHLRAVFFDFDGVILDTETMLYKSWCHIAGLYGVSF
                     PLKEWAANAGGYQYHIFDPVTHIETEAGITLNRTEVNDRRRAWFLEKVAALPEAPGIR
                     ESLESAASMGLPLAVVSSSGQEWVEGNLERLGLLSLFETICCGSEVDAVKPAPDVYQL
                     ALQRLNLTPGQVCTLEDSPQGIAAAVAAGIYCIAVPNQVTRAAALKGYQRRIDSLTER
                     PFTELLLDIEKDLSRAL"
     gene            19651..20904
                     /locus_tag="PKN92_05655"
     CDS             19651..20904
                     /locus_tag="PKN92_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNZ48312.1"
                     /translation="MRFESFVAARYLRGKRRTRFISLITIISIAGVSVGVTALIVVMS
                     VMTGFDNALRETIIGNRAHLTVANPMGRPMENVEEVIGDLMDLSPEILGAGPIIQAEA
                     LLKRNKYTTGAFLLGIDPEKEQGVTDLAQNLTRNGGRTFGEGDMPGDKEIVLGFRLAH
                     RLAARVGSEIAVITDKPTVTPFGMRPGNQVFLKVSGISQAKMSDFDNLYAFVNIETAR
                     MLTGRPGVDGIHLRLSDAFIAEPVARLIEERLPYRAETWYQSQEAFFEALKQEKVVMF
                     IILVFIVLVAAFNITSTLIMIVMEKRRDIGILRTLGSSSRSILTIFILEGLMIGLSGT
                     LIGLILGVLLAYNINAVAEFIAGLLGMDLFNSTIYYFDGIPVAIVAWDITWITLSAIV
                     LTFISTLYPAWSASRLNPVDALRYE"
     gene            20897..21592
                     /locus_tag="PKN92_05660"
     CDS             20897..21592
                     /locus_tag="PKN92_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48313.1"
                     /translation="MNNQVHPILACRNLRKCYRDGSRELVILDDISLEIPEGEIVAIS
                     GPSGVGKSTLLHIMGTLDQPTSGQVYFKGDNLADMSQAKINRLRNQHIGFVFQFYHLL
                     PEFTALENVMMPALSQGMHRRKCQEPAAHLLEQVGLSDRMTHKPGQLSGGEQQRVAIA
                     RALFNKPELLLCDEPTGNLDESTGEGIIELLWTLNQSSHVSFVVVTHDETVAQRAGRW
                     IALHDGKAFTRNG"
     gene            21625..22809
                     /locus_tag="PKN92_05665"
     CDS             21625..22809
                     /locus_tag="PKN92_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48314.1"
                     /translation="MPEDRPSKDTAVPETPRTFTLRKTIIYSLLPTFLFLGGLESCAR
                     LYELWHPPLTLDCGWGFNEDSRIFIPAGIQRNKMTTRPEKVISFVKQSFLMPKPAGTY
                     RMIILGGSNVNYMQMNLKVMCKRLEQYPGETRHFEIINCGGLAYGSERLRIMTQELLQ
                     YTPDLILIYAGHNEFEELIHKNLTNLNALPIQKTAYSLAMLRTLRDFVATVQLTFLDP
                     QKLRETLPPEVDWSTGTYEFSQEELSAHMERYRENLDAIVSTFRNSNIPVLISTVATN
                     YREPGLHPAQKIIQQEIADLYAQGQYEAGLTLARETLSRSARHQASDVENGIIRDIAE
                     KHHLPLIEGEKLIMEAEPHGVPGETLFSDRCHITDTGRNIIVAAFEKEIHIIAAHPDS
                     SK"
     gene            22840..>23459
                     /locus_tag="PKN92_05670"
     CDS             22840..>23459
                     /locus_tag="PKN92_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48315.1"
                     /translation="MLRWFKKTLFIFLCFGLLAVVLFFFLEVAARLYQSHIEKHNPFI
                     IAANKGEELPYREMRTENDFQSLLSSKMQGSDRKNENEPKLHPDTRILLNDWLDSKGF
                     SPEKRMKLREDFRSWSTQTRDYYACLTHEAVIVFNAENTIRNYFGSDASYFEGYQRSI
                     TLAYLHQTGILGRIYEALKEVRHTNKTVTLTLPWSEHIPGSPVFTA"
BASE COUNT         5855 a         6154 c         5875 g         5575 t
ORIGIN      
        1 agcccgcctg cagccacaat agcatcatgc acacggctat cctcttcaaa ataataaatg
       61 cccggcctcc gtactgcacc ttcgacagca acgcaaagtt tattttcggg cagagccgcc
      121 gtcacgggct ctgttttcgc ctccgctgaa tatatggctg atccacccgg ctgctcgatc
      181 ctctccgggg cggggtctgc tgcactttcg ctatgcggcg gaagagtctc cagcgaaaca
      241 agttccggct gtgcttcatc cgaagagaat aaaccgtacc ctgccaatag acccagaaaa
      301 atcccggcag aaaggacagc aacccccgaa ataatctgtt tcccggttat tgaaaaggat
      361 tccatgccga ctactccccg atataaatca gcccagtata tcccgtctct gctcgaatat
      421 ccaaaatcac taaataaaag aaattttgaa ccttcatttg cctttcttca taatgaacct
      481 gagcagcctt ttcacacaaa gcagaccgga gcgacaatga ttcaaaaact gagacttctt
      541 ttatccgtat acctactcat cggaacggtg ttcgcttcca gtggaatcgc cgctgttacc
      601 ctctccaatt cgcatatgcg gcttggcttt gacgaaagta ccggcgcatt gattgaagcc
      661 tgtctggcag aaaccggctt taacctggtg ttgcctcatg ccaataattc gctctggcat
      721 ctgctgcgcc cggatggaac atttattacc ccggaagacg catgctcttt ttcttttaca
      781 aaagcccctt cagaaaacag tgtggaattt ctttggaagg gattcgacga tccccgcagc
      841 cggaatctgg aagtgcaggt ttctgtcacc ttacctgaga ataaacagga aagccactgg
      901 aacctggcac tgcatgacag tgatgatttt ggcttctatc agattgcgtt tccgcgaatg
      961 acttctctcg ctgctcaaaa aaatgaagcc cttgccgtgc ccttctggat gggtgaagta
     1021 acttcttccg cccgggaatt attaaatcct gccgatgcac cggcactccg ccgaagctgg
     1081 gaatacccgg ggcttctatc gctgcagtgc ctgactttct acggagatca gggcgggctt
     1141 atgttgtcca ctcgggacag tactgctatc cggcgggaat ttgctgtttt cggaggcgag
     1201 aggaatgcgc tggggattga gattagtcat ccggtgagcc ctgcatcggc gaactatcag
     1261 ctgtccggcg atgtcctgct gtcactgttt accggcgact ggtttgacgc cgctcttcta
     1321 taccgtagag agcgcgacgc acttccttat gcggaacaaa gccgtgttcg cacaaaccaa
     1381 attccagact gggcggaaga aacagggctt tggatctgga atcgggatgc ctctccggga
     1441 gttctctccc ctgctgctgc tctccaaaaa gaaatggcag cacccgtcag tgttttctgg
     1501 cactggtggc acggatgcgc ttacgatgcc gggtttcctg agtacctgcc gccaagagaa
     1561 ggagcagagt ccttcaaaca cgctctgggt gctgcgcaaa aagaaggcat acacgcaatc
     1621 gtttatatga accagcgctt atggggcatg gatacaacga gctggaaaga aaaagatgcg
     1681 cagcgctacg ctgtcaaagg ggcggacggc acaattcgcc gcgaaattta caacacattc
     1741 acccaatcgc cctgcgcttc catgtgtatg gggacatctt tctggcgaaa cacttatgcg
     1801 gacattgccg aaaaggtact gctggaattg ggtgcagacg gaatttatat ggatcaggcg
     1861 tgttccagcc ttgcctgtta tgaccaggaa catccgcatc ctccgggcgg cggtccctgg
     1921 tggatgcagg gctttcagca attggcttcg gacatccgac agcgctgtct tcccgtcaaa
     1981 gcgcccgttc ttgccgggga aggctgcggc gaagcctggc tcccccatct ggatctgatg
     2041 ctgagccttc aggtcagcct tgaacgctat gccgcacccg ggcagtggga acctattccg
     2101 ttttttcacg ctgtttatca cgactgcgcc cgttttttcg gtaattactg ttctctgacc
     2161 cgcccgccct atgattcatt atggcctaaa gaaagtgcac cggatcggcc gctggcgctg
     2221 ctggacaaaa aatttgctgc gcaattccga ctggagcagg gacggtcttt tatttggggg
     2281 caaattccct gcatcgccaa ttttctgccg gagcaactca ccagccgaag ggaagagctg
     2341 tcttttctaa aaactcttgt ccatcttcgc cttaaaacat tgaaatatct gagagacggc
     2401 attatgctgg cgccaccgga tattggctct ccccttattg aaatcccgac ctcacgctta
     2461 tccatctatg ccggacagca cgatgcggtg caggaatata cacgcctggt tccttctgtt
     2521 ctggcggcag cctggcaggc atcggacggc aatgtagctg ttgcgctggt caacatcgga
     2581 gacagcactg aagagatcga gctctctctg gagagcagcc ggtacccgct gggagaaaaa
     2641 ggaaaggttc gacggatcac ggccgataca gaggaagaaa tggggctgtg gacacaaggg
     2701 caggcgctgc atgcactcac tctggaggga agttcgcttg cggttgttga atatctttgc
     2761 gaaatggaac cgtaaccaat accaaaacag aaacgggagt cgtgctcatg gaggctttat
     2821 ttctttccct gttatttgct gtcagcacag tgcatcttca tgaaaatggt acagcgctaa
     2881 cggtagaaac cgactatgtg cgctgggaca tcggtacaaa aggagaaacc cggtctttgc
     2941 tggataaaag cacggaagaa gaactgatgt ccgctgaaaa tcagaaagcc ttttcttccg
     3001 ttttatttga gggaaaagaa tggaaggcga ctgagatcag cccgtccgaa aagggatatc
     3061 gcattttata tggagcaagt ggcgtggagg ctgagttccg gatccaatcg tacccgcatt
     3121 atttttcgct gtccgtccac tcgctgaata atccctcggt ggaggccctg acgctgcttg
     3181 acctttcttt gaatcttccg gcaggtgaag aagaaaaaac aacagcttgt gtccttgccg
     3241 ccaatctccg gacgcatgtt cccgatcttc cggggcctgc atcgcgtctc cgtgcctccg
     3301 ccgtcaaggc tttcgggatt gtcggcgcac aggtcaatat catcctttgt ccgggagcga
     3361 tgctgcgccc gatcatgaaa gaggccgttg aaaactcccc tgagctgccc cggcataaga
     3421 atcaatctgt tccgcccgtg ggcggaccct gggcattgga cgcttcggca aacaaaggat
     3481 cctatctctt tgatttcggc acgctcactg aagataccgt tgatgaatgg atcgacctcg
     3541 tacagcagct cggcttcagg cagatagact ttcatacggg aacctcgctg cgctttggcg
     3601 atctgacacc caacgaaaaa cttttcccgg aagggcggaa gagcgttaaa agcgtcattg
     3661 accggctgca tggggcaggc atagctgcag ggcttcatac ctatgccttt tttattgcca
     3721 agaactcccc ttatgtgact cctgtgcctg atccgcggct cggcaaagat gagcagttta
     3781 cacttgacaa agacctaggt gagacagggg atgcgctctt cgtagcagag tcgacggaaa
     3841 aggtcaagac agacacaggt ttttttgcca gaaacagtgt tacgctccaa gtcgagaatg
     3901 aactgatcgt tttctccggg gttacacggg agcccccttt ttcttttacc ggctgtgtcc
     3961 gtggcgccca tggtacacgg gcgtcatctc atgcagcggg aacacctgtc tacaaattaa
     4021 aggaatgctt cgggctcttt ctgcccgacg gcaattcaac gctgctcaca gaaattgctg
     4081 ccgtaacagc ggacacattc aatgagtgcg gtttcgacat gatttacctc gatgccctcg
     4141 acggatccga tattttcgaa ggcccggaaa attcctggta ttatgcgccg aagttcgcct
     4201 tcgaaatagc acagcgcctt gagcggccgg ccttgtttga aatgagtacc atgtcccatc
     4261 acctgtggtt catccgggca cgcatgggcg cctgggatca cccggcacgg agccataagc
     4321 gcttcataga tctgcattgt gaagtgaacc gacaaaacag cactatgttt cttcctatga
     4381 atctgggttg gtgggctgta aaaaattggc agcctggagc cgcttccgcc tggtctgagc
     4441 ccacctggcc ggacgacatt gagtacctgc tgtgtaaagc gctggcaaat ggtgcgagtc
     4501 tttctctcat gggagtcaat ccggacaatc tcgacacaat gcctgctttt gaaagactgc
     4561 tgccgctctt taaaacctat gaaacagccc gcctctccgg catggtggca gaagaaatta
     4621 aagagcaact gagagcaccg ggggcagaat ttacgttaaa aacaacagac aacgaaagct
     4681 tttcctttta tcccgctgcc acatataagc atacagcgga cttttccgac ccattcagtc
     4741 ttgactgggc tgtagaaaat ccttttgatg aacagccggt tcgttttcgt attgaaaccc
     4801 ttctctctcc ggcttctttc gattcgcagg aagcacaagt actgctggac tacacccgcc
     4861 cggaagcatt gaatgtgcag aaaaataatg atggtgtttc cgccgaaatc gccatcactg
     4921 aaagcccgct gaacgaaaat gagcacgtag ccgcattcac actgctcagc acccgggaaa
     4981 atgcacgggg ttcctgggcg caaataggcg ccgcattttc gccccctgta aacgccggag
     5041 ccaaggcagg gctcggatgc tttgtctacg gcgatggaag cggagccctc ctcaatttgc
     5101 agactctaag tgcagcccat acggcggcag gtggtatcgg cgaccattat atcccgctgg
     5161 actttaccgg ctggcgttat atctctgtag ttgaatttga aagcggcgct atcccgcaat
     5221 ggcactggcc ctatggcgac cagtacagta tttacaggga acatgtggac tttggcgcac
     5281 tggagtcttt ttctttctgg ttcaacaatc ttccgaaaga caaaacggta cactgcaaaa
     5341 tcgggccagt caaagccctt cctcttctgg agggatcttt aaaggaatct gccctgatca
     5401 ttaacggatc cactctttca ctgccagtaa ccatctcatc gggatgctgc ctggaatatg
     5461 acccgcaaaa gggcgcattc ctgtacaatg aaaaaggaga ggtgcttgcc cagttttcgc
     5521 cgtcagagac tatactcctg aagaaaggaa caaacagcat ccgcctgaca gcagccaacg
     5581 gggcggcgct tccggcggcc cgtgcgagaa ttaccatttt gacagaagga actgtccctc
     5641 tccattaaaa tcacatgaac ataaaagcgt ccagaccaaa gaaatagcct atgctttccg
     5701 ggcttttgcc acggttctca aagcggagga tgtgcttccc tggcgtgcag gcgggcacca
     5761 aaaagcgaaa ttctttctgg ttaatgcctt ttccgtagag attaaccggc gctccgagcg
     5821 gtattccgtc cagaagcacc tgaaaagtcc cgtaatcgcg ggcacatgtc agcatcagcg
     5881 caagttcctg tacctgatcc ttgtcaatat ccacttcaag ttccagatac ccgttgggct
     5941 cctgaggtgt ccagaaaatt tgcccgccgt tatagcctat ttccggctgg acagcaaagg
     6001 ctccatggga gacggagcac tgatcaattt ttgtttctgc ttcgatcagc tccgcccatc
     6061 cgccgtacac acgttccaga gcgggcgcta gcgattcata tgccttatgc ggttctgtct
     6121 gataccagaa agcaacagaa gaaaaatcat cgtcccgttc tgcaaaatat gagctgattg
     6181 ttccgtcctc gttataagta acacccatat gttcaatttc aaggcgcagt gatttttcaa
     6241 agactacagg atccggaatg tgccaccggt agcaggaggt tcgtgcgagg gattcatagc
     6301 cctccatgag aggggctccg taccagggcg ttgacatttc ccgcaatccc caggcatcgc
     6361 aaaaataatc ttcggaaccg gtaccacgaa gacgtggctt ttctgcacca tcgataaacc
     6421 agaaatcgtc gccttcgccc caccagtgcg ggaaatgctg acggacattc agtactgtgc
     6481 ccacataatg cccacgccct tcgatatcag caataaggta gtgtttcccg gatactgccg
     6541 gatgttcctg ccggtacatg gcatggaaat aagcggtatt ttccggaagc cggtcaactt
     6601 ttgtccaatc tacataataa taaaaagcat ggacaggccc gtcaccttcg ttagttactg
     6661 taatacgcgc tgactttctg aaaggcatgg gccagtagca atttctggct ctgccctcag
     6721 aggtcaccgt tacaggaaat gattccatcg gatgatccag cccatgcccc atggcaaaaa
     6781 aatcacccag aggcacttca acagaaggat tgctttcgcc atcccagtac atgcgcaaaa
     6841 ccagcaggcg tgaataaccg cgatcgacag cagccacggt attccatata tgctgaatca
     6901 cgcccggtcc ttccaaatcg gcgataacga gacatccgcc gggcgctata aaacgggcat
     6961 ctgcgttccc attttccaga tcggcatcac aggaagaaga gcgctgcgtg cttccttctt
     7021 tgatcaaact caaatcggac agaaaactat tacccgcctg catcggcgtt tgatgctgac
     7081 aggcagtcat acagaagagg cacacgcata ctataggcag agacattttt attttcataa
     7141 atagcattcc ttctgttccg gtttcagttc gtttgtcgca gtcgatttta acattattct
     7201 gaaaaaccgt caagacaaac ttttatcttt tgtacaagag atgatttcta tgttatggtg
     7261 attttcaata gaaagctgac agactttttc ggcagaccga aagaaaatag ccgtttgaag
     7321 aaggaacgta ttatgtctaa agtgaagctg ttcatctgga ttctggcatc tgttctacta
     7381 tgcgcaccag ttgctttttg cgatgcggac gcagaaaaca atgacgattc tctggaaaac
     7441 acactggacg aacttgctgc cttgggactg gatcccgacc ttattgaacg ggacagtttt
     7501 cgaaaaggaa ccgccccgga aaatttcaaa gaacggtcag gagctgagta caaaccgtct
     7561 cacaatgaag aaccgaatat tccggcacaa tgctggatcg aaacaggcta cggaacgcag
     7621 aatgcctgcc ttttctgtca tagcaattac ctttccaaag aaaaacacgg caatagttat
     7681 cctattgccg acgaccagat tttttacagt ttccccacgc ctgaactgaa ccggattctc
     7741 tggcggaaca ctatttttcc gcaggagatc agcacccgtc ttgaaaaaga aggaattgtc
     7801 attcccgatc cggaggatgt cagttacatg cggaaagata actggaaaga gctttacgac
     7861 agggtgcgca cgaactatcc gaaacgatgg ctcattcccg atgacgacga atttcttctt
     7921 ttcccggcat taaatccggc acatctctat cctgaaaaac agccggatcc tacagaagga
     7981 aacaaacacg gctatataga tcaggaaggc tttgtccgtg acgagcagaa cgactatacc
     8041 ggatggcgcg ccgttaactt cttcccttac ggcattttca cccctctgac cgggagcgtc
     8101 agcggtatct atatccggct tcctgaaatt ttccggacag atgccgccat tgtcgatgtg
     8161 acgttatata aaaaaaatct ggatattctg gaacggaaca ttaaaaacca gaaactcaca
     8221 gagaaagcat atctggggga tgccagcggc gttcccgttg aaaaaggatt tttccctgct
     8281 ggcacagagt ttgcccaccc gctccattat gtggatctga atgcggacgg cgaatcagga
     8341 gaacggctgg acggggtggc ggatcagaag ggctccgatt atgaatttcc gggcacccgg
     8401 tcaaaacggg tcaaagaaat tcgttatatg tacaaatgga aatctgtcac tctggaagaa
     8461 atcaccgagg acgcgcatcc ggagaatttg gtgctgggtc aggaagggca aggctggatt
     8521 gacaataaca tgggatggat tattgccggt tacattgaaa atcgacaggg agcgctccgg
     8581 gcacagacta cagaagagct gatgcaatgc ctcggctgcc acggcatgac cggaaacaca
     8641 gtggacagtg tctggtcttt tccgcggaaa ctgcccggca ataaggggtg gcgtgacatg
     8701 aattatggcg aatataattc tcaacaccct gacagaaccg cacttcctga ctacaggcac
     8761 ggaaacactg acagtggaga attcggattt ttttataaag tagtggttgg tgctgatctt
     8821 tttgggatta tgcctgacga agtggcaaat gagctcagag cttatgcgaa aaataaaggc
     8881 ggcagtctgc agccggaaga agaccttgac gccatccttg atgatcatgg gcttattgaa
     8941 atgccccgag atcagcgcaa aaacttgctg acgaaacggc agcaactgat gcgctctttt
     9001 gtcaatgacc gtgcctatct agcaaaagcg gaaggatcag aagacccgtg catcaagggc
     9061 actctttttt atccttccga gtccacaatg aaaaagaatc ttcatctgta ccggcagatt
     9121 gttcttgacc agagttttaa tctgggcaaa gacattttcg gctctgaaga agggcatgtg
     9181 cccttctctt ttcgctctga cggcacagta aaaaacatta aaggagaaat cattccggcg
     9241 ggagaaataa tcaccagccg gcctcatgac aaaggcggaa aaggaacaac gcccaccggc
     9301 atcgcttccg tggatcagga cggcaatctg gcgggaacgg acggcaaccc ggtggatatg
     9361 gatgcatcac cggaaaaagt cgggggacac atctccacag gcggaacctt cgagcctttt
     9421 tacaacccta tactgagtga tattccttta acagaaccag gtgcaaagta aaaatcagac
     9481 gcgacagata gcgattgcct gacgaagcga ttccagcgga tccggcgctt tgggcacaaa
     9541 ctcatgagcc agaaaaccac tgaagcctgt gtctgcgatg gcagaacaaa tccggggata
     9601 attcagttcc tgtgtgtcat caatctcatt acgcccaggg actcctccgg tatgaatatg
     9661 accgatatac ttgatgttct cttcaatcgt tgcaatgaca tccccttcca taatctgcat
     9721 atggtaaatg tcatagagga gtttaaagtt aggtgagtcg agctttttag caagtctggc
     9781 gccccaggca gtgtgatcac attgataatc tttgtggttt cgcttgctgt tcagcagttc
     9841 catacataaa accactcctg ctttttctgc aagaggcaga atttttgcaa gccctttggc
     9901 gcagttttcc aaccccgttt catcatcctg accacggcga ttgccggaaa agacaaccat
     9961 gttgggcaca cccaagtctc cggctttggg aatcagacgc tgcgcattgg caatcagttc
    10021 ttcgtggtgc tctatccggt tccagccttt accgatattg ccgggcccgt ttgccatggc
    10081 gcacaccagc ccctctcctg ctaccgtctc ccaatcccct tcgctcagca aatcaatccc
    10141 ggtaattccc ataggcttca ccgcctgaca gaattccacc agagggattt tgtcaaaaca
    10201 ccatttgctg accgactgtt tcaggcgccc gacatccaca gcggcctttt cctgcgccat
    10261 gccctgaggc attacaagtg cggcaccgga tacggcggca agcgccgcca gcatgtttct
    10321 tctggtcatc tcggaagtac tgttcataac gcaaagcctt ctcatggtat ttcagggtaa
    10381 aaacattcaa cacacacctc attctatgag aaggcatttc agcaagtcaa caccgatgcc
    10441 acaggaacaa gcgattctga aaccagtgct tccgactctg cactcaccgg caccgctgag
    10501 aaaaaaaaga tacagcgcgt ctcttttgtt attatagagt tcaatccggg caacaccatc
    10561 agtttacaca tatatgcacg aaaggaaacc atgcgtcata caatttttat ctgtaccatt
    10621 tttatcattt tgttttcatc tgcccttttc gcttcagaaa actggcattt atgcgttctg
    10681 cctgaaccag cctctgaaga ggctgtgcga ctggccgttg aagacctgcg ccttactgca
    10741 gactccctct ccataccgct caccatggtg gaagatgcag aaaaaacaga aggcaatatc
    10801 attatcctca gctatgcccc gcaacaccct cttattgcaa agctcatcgc tgacgggcgc
    10861 tgggctccgg cactcccgga caatccggaa ggttttgaaa tagcaacagt ccccgacgcc
    10921 ggcagaaaaa tcatgcttat cgccggagcc tctcctgtcg gaaacgctta cgggctttat
    10981 tggctgcggg atcgaatgcg ggtataccgc accgttcctg acatcaacgc tgtaaggcag
    11041 ccgcaatttt ctgtcaggct cggcgcagcg tggcatcggc acggttacgg cggaagcacc
    11101 aaagagcaga tgcgcggagc gctgcgcaac agcatcaact gggtttccgg cgcaaacatt
    11161 ctggatctga tcccctggca tgcggaaccg gaagcaactg aaaatgcccg gcatcgggaa
    11221 gaaacccgag aattgatttc ttatgcccac agcctcggca tgaaatattt cagcttttcc
    11281 aatgcctatg tgtatcatcc gtctattctg gaagaattcg gcgccacgct gaatcctgaa
    11341 gatccccgct tctgggatgc cgtgcaggaa acatatcgcc gcctttttca ggcgctgccc
    11401 gagctggacg gcatcgagtt gtgcaatgat gatctctccg gcttctgggg gaattacctg
    11461 ccttttgatg tcatgcatga tgcccctgaa tgcgactggt cttatgttaa acggtacaac
    11521 actttcatgc aaaaggtgca ggaagtggta tccggcgaat tcggaaagga gcatttttat
    11581 tttacctggg gacttcggga gcacgaaatc cattgccagc cggaggtttt taaagctgtt
    11641 ttccgggaaa atatcccgac cgaccattta tacctaatgc ccaagatcac ccggggagac
    11701 cggtggtggc accagccgta caacgcgacc ttcaatctga cacctcataa aaccgttgtt
    11761 ctttttgaaa ccatgaacta ctatgaaggc ggcgcatccc ggctcttccc cactttttcg
    11821 ggtcagtatt tccagcgggg tctgcagcgt tttctcgcgc cggaacacag taatgtggaa
    11881 ggcatagcat tgctgtccgg cccgctccgt gaaggctgga gcacgcaaga cgcatacagt
    11941 tatgttcttt accggctcac ctgggatgta gacgaaccga tggaagatat tgccctggac
    12001 tttgcccgca tccatttcgg agaagcagcg gcagcgcata tggcgcagat ctatctcgct
    12061 tcaccggcag cgtataaata cgggcttcac atcgaaccga tttcctacgg gcaattcaat
    12121 tcttttcccc atatgcgcgt aaatactttt ccggcggagg gctaccctgc tctggataac
    12181 ggaaaagaac atctggaaaa tttgcataag atctacctgc gatgtgaacc atgggaagaa
    12241 gccactctgc gagcgcagta ttatggtctg gagcaggccg aatcgatgct gcgccattat
    12301 gactcagctc gttccgctat tgaagatgac gcgctgtcaa aggaagtcgg tgaccggttg
    12361 cacatgacta aaaacctgat cagaaccaat atcgggtatg tcaccacgat gttcgcctac
    12421 ttcaaatata aagaaaacgg gatcccggaa aacagagagg ctttgaagaa agcgattgca
    12481 cagctcgccg aggcaaaaaa tgatttcata aatacacccg gcttcggctt ccagctcttt
    12541 gcggtcgata tactgcagcg caatgcggaa gccctgtacg aaaaccgaga agaagccatt
    12601 cgccactaca atgcaacgcc cagtcgtgag gaactggatg cccggattaa aaaagagcag
    12661 gccggatatc gcgagacctt agcaaaaaac aaagacaaag ctgtgctttt tgcgaagttc
    12721 gagctgctgt gtgacggcca ggatctggtg attattcagg gagaccagta tcatattaaa
    12781 aacctgtact gggacggtgc gcatcccagg aaagcggaat ttctgacacc gcttccgaaa
    12841 aaagaagtgc aggtggtcat tgatgatctt gaatcacgcc cgatgcatcc ctttatcctt
    12901 gaacagccca cagccgacaa tgactatacg gtgaaggtgt atctggatga tcagccggga
    12961 ggcaacggct ggatgaattt tgatctttac tatctggata aagaaagcgg catgtaattc
    13021 aacgaagttc ggatacgaag caggctaaca gcaatttcca tgaaaatatg catcctaaat
    13081 gtgcttcatg atgctctgga caagcgcatg tatcataaga tcgcccgaag tttgtccgct
    13141 gccagtcatg agattgtctc cattgctccg cctgctgcat tcgctgccgg aaggaatcag
    13201 gcagaaagcg gcgaaacatc tgttcgattc gtttttacgc cggctgtgca ttcttttttc
    13261 gggcggttgc tgactgttct gcgcctgatc cggctgggac tccgcgaaaa agcgacgctg
    13321 tacatcgcac ctgaaccgga atcatgggtc gccgctctgt tgattaaagc ggcaacagga
    13381 cgaaaagtcg cctttgatat gcatgaatat gtaccgggtg aatttgccaa gtttttcccg
    13441 tcctttctga gaccaccaat ggaaaaaaca acctgccgga tcatgcgtct tatgggaaga
    13501 gcctccgacc tgatcatcct gacccgccgg agttttgagc ctttatggtc cggtattcgg
    13561 acgccccggg tcgtggtcat taacagcaac tggctgcaac cggcatgtac cgccgttcct
    13621 gatgcagtcc ggaagattgc aggcgaaaga ccgaccgttc tgcaccaggg cattttcgga
    13681 gaaatccggg gttcactgca gctgctggaa gccatgaaac tggtgaagga agtgctgccc
    13741 gaagcacgct gtctggtgct cgggcgctat gtttatggcg acgaaagcgc atacaggcag
    13801 aaaatcaagg aatcaggact ggaagacacc cttctgttgc ttgatgaagt tcccttcgaa
    13861 gaggtccccg cttacatcag tgcagtgcag gttggattaa ttctgtttca gcccggcctg
    13921 cgcaatcatg aacttgccat gccgcacaaa ctctttgatt atatgcggga aggcgtcccg
    13981 gtgatcgcac ctgattttgc ggtcgaggta gcgcatatcg tgaaagaaac acaatgcggt
    14041 cttctggtgg atgtcacttc tcccgatgcc atcgccgatg ctgtcatcag gcttctcacg
    14101 aacccagacg aagcacaacg cttcggcaga catggacgtg aggcggtaga atcgaaatac
    14161 aattggcaga aagacagcgc cgctttagtt gacgctattg cggcactgga gtaacggtat
    14221 gggtggtctt gcgggctata cgggatgttc cgatgaaagg ctgattcaca ccatgatgca
    14281 tcagcaaaag tgccgcggga tcaggcacgc ccgtttttta tcccgtcggg actgtaccct
    14341 gtcggatttt gagccagaaa catctgattc cggcattcgc tcagttgccg ccgtgcttga
    14401 cggttttatt tccaacagga agcaattgca ggcagaactg aaagcagagg gctatccgta
    14461 cgagtgcggt tcagacactg ccctgctaca gcatctctgg actgcccggg gcatttccat
    14521 gctcaatcgc ctggaaggaa tgtatgcctt cgctttatgg gatccgcaag agtcgtgcct
    14581 ttatctggtt cgcgaccgca cggggtcacg accgctctat taccatgagc ggaaagatac
    14641 ttttttcttt gcctccacaa ttaaagggtt gctgggctac acaggaatta gaaagacgct
    14701 tgaccttgag ggactggacg actatttcgc atttctgcat cccctgccgc ccaggacgtt
    14761 ttacaaagaa atccgtcagg tccctgcagg gcactatgcc cggcgggctt caggacaatt
    14821 agaagtaaag cgttactggc acccgatttg tcagccaaaa gaaaactgtc tgcttgagga
    14881 atgggttgtt tcaaccgata cgctcctgga gaacattatc gacacctatg ccgacgcaga
    14941 agcctccgga ggcgcgctgc tgagcggcgg gctggattcc acggttgtac tcgcatacct
    15001 ctcgcggcat ctctccggtg tgccgtcttt taccctcgga ttcacagaag aggaagcgca
    15061 ctatgacgaa agaagggctg cccgtaccac ggcttcactt ctcaaaagcg cctgccatga
    15121 agttttcata caccccgatc ttccgaataa tgcctctttt atcctggagc actttgatga
    15181 gcctttcgga aatccaagtg ccttgctcgc ggacattctt ttccgtgaag cggcaaaatc
    15241 cgtttctgtt gtttttaccg gagacggtgg cgatgaagct ttcggcggtt atccgcgcta
    15301 ccgagggctc ctttgggcag ggctctacag gcatatgccg ttttttcttc gccgtcacgt
    15361 tctgcatccg ctgattcata tgctccccga gcgtgcgggc ggactacata cattgaggag
    15421 gctgcgaaga ttttcagaag gctcttttct tgaccccgtt gactgttatg ccctttggat
    15481 ggagcaccag aacagtaaag aacgtctggc tctatacaac agcaatctca ccgaaatact
    15541 ctgcgggcgt gatgcaacgg aacctgtccg ccagctgatg cgccagtgtg acagcccgga
    15601 catcattaca caggcgctct atgccgacat ctcgcttttt cttccggcga atgtgctgtg
    15661 ttacggagat cggctgagcc atgcccacgg tgtggaaatg cgtgcacctc tgtgcgaagg
    15721 tgcgctgttt acccatcttc tgcaggcacc gtcgttctta aaagtgcaac attttgccga
    15781 taaacagctg ctgcgcatga tcctgcaccg tcagcttccg gaaagaaaaa tatccagaaa
    15841 aaaaagaggg ctcaacccac ccatgagtat ctggctgaac agatggcttg aaaagcccgg
    15901 tgcatgggag cactttttgc aagaaacaac tgccgttgac ctcttcgagc ccggaagggt
    15961 ctgggaactt ttaacagagc atcgttcagg tctgagagat cacagctggc gattatggcc
    16021 gctctactta ttcgcacgct ggttcaacac ccctgatcct gttctgccaa cgtattaact
    16081 tgctcaaaat acccgatttc aggtattctt aaccagtcta tccctcattg ttccgctgat
    16141 tttccagcgt cattcttaat tcaggggaca aaccatgtct actacaaaag aagttatttt
    16201 atccggtatc cgccccacag gccgcctgca ctacggaaac tatttcggag ccatcgccca
    16261 ctttctgcgg ctccaggagc gggacgccaa ttgctactat ttcattgccg attaccatgc
    16321 attgacaacc attacggatc ggcaggaaat ttaccgatcc acactcgaca tgctgcgtac
    16381 ctatgttgcc tgtggccttg accccgaaag atccattatc taccggcaaa gcgatctgcc
    16441 cagcacggct gaactggcgc tgcttctggg aatggtcacc aacataggtc atctcgaaag
    16501 aggaacgacc tataaggata aactcggtcg tctgcaggat gatcccagaa gcgagggcaa
    16561 tccgctttcc tatggtctgc tgggataccc cgtcctcatg gctgccgaca tccttatcgt
    16621 ccgcgccgat ctggtaccgg taggggatga tcaacgccaa catctggaaa tggcgtgcga
    16681 tatcgccaca aaattcaatc accgtttcgg tgaaacactg cacatcccga agcctctggg
    16741 cagagatgcc ctgcgcctcc ccggactgga cggatcagct aaaatgggca aaagcgagaa
    16801 caacacgctg gatctgctgg acgaccctgc aacagtactc cgcaagatca aggcggtacc
    16861 aaccacgaca gatccccttc ctctggaaac cgagagcaat gcaccggaga tagtgcttcc
    16921 aaaaatgccg gcgggagtag ccgtgctcta tcgtctgctg tcgcttcttg cgccggaaga
    16981 agcgtatctg gactttctgg agcaatacag gcagggcgga aaattttacg gcagcctgaa
    17041 aaaagaagtt gcccgcttcg tgtcggaatt caatgcgccc attatcgaaa aatataacga
    17101 ccccgcccat aacgacgctt tcattgaaga tttccttcgt gaaaatgcca aacgggtaac
    17161 tccggtagcg ctggaaacgg tggaagcatg ccgggaagcc atggggatcg gtcacagcct
    17221 tttcagggca taaaaggagc cgcagcatgg atgtcattcg cattggattt atcggactgg
    17281 gcggcatttg cagacagcgc catgttcccg gcttaaaacg tattgcgggc atagaattcc
    17341 gtgcagtgtg caacagcagc agggcttctt cagaaaaagc cgctgctgag ttcggcattc
    17401 caaagattta tgagcaatgg gaagatctgg tgcaggcaga cgacatagac cttgtgctaa
    17461 ttggtgcctg gccgtatctg caccatgatg cttctatcgc cgcactcaag gcagggaaac
    17521 atgttttctg tcaggcgcgc atggcacgaa atgcacagga agcgcaaagg atgtataccg
    17581 ccgcacagac tgcgaaaacg ctgacagccg gtctgtgccc tgttcctttc ggactgcgct
    17641 atgaccggac aatgacgcgt cttctgcaag aaggaacgct cggttcaatt cgtctggtga
    17701 atgtgcacag cctctcgcct ttgtggatag atccggcggc accggtcaac tggcgaaaaa
    17761 accaccatct gtccggtctg aacatgcaga cgctgggcat gtacatagaa gtaattcacc
    17821 gctggttcgg cttgacaacc cgtgcatcag cagaaacatt tctctatacg cctgtacgga
    17881 aaaatacaga cggaactgac tttaagattg aaattcctga tcagattctg gcaagaaccg
    17941 tcgtcggaac agacttgccc gttacttatg aaatcagcgg tgtttccgcc cttgcccgtg
    18001 agacaattga tatttacggc gaccgcgccg ctctgcatta tgatgtagcg tcggacagtc
    18061 tgtacctctg cactcaaaat gagcgcgtac cggttacgcc actgccggaa gaagagtatg
    18121 atcttgacaa ctggcgtgtg gaagaagatt ttattaacgc tatacggcag gggctcccct
    18181 atcatccgga ctttaaagat ggattgaatt atatgcaggt aattcaggcc atctatgact
    18241 ccgcaaaaaa cagcagcgcc gccatcgcta ttcaaaacct ttaaaagacg gggtaacggg
    18301 tatggataaa accgctatca ttaattatct gatcaaacat ggcatcgtgg cagtagtccg
    18361 tgctgattcc ggagaaaacc ttgtcccgat tattgaggct gtcatggagg gaggcgtacg
    18421 ctgcatagaa attacaatga caactcccgg tgcgcttgag tgtttggcat tagcttctga
    18481 aaagctgtca ggggccgatg tttctctcgg tgtcggaagc gtactggatg gagaaactgc
    18541 ccggcttgct attcttgccg gcgcccagta tattgccagt cccattgctt ctgctgaggt
    18601 aatcaatatg gcgcaccgct acggcaagcc ctgcatgccc ggcgcttaca cgccaactga
    18661 aatttatact gcctggattc tgggcggcga tatcataaaa gtcttccccg cgggatgcgg
    18721 cggtctggaa tatattaaag ccgtccgggc cccactcccc cagatacccc tgattcccac
    18781 aggcggtgtg gatctgagca atctcgctga tttcatcaaa gcaggcgttg cgctgatcgg
    18841 agcgggcggc aatctggtaa gtaaaaaact gatagaggca aaagattatc aggggcttac
    18901 tgagaatgcg cgccgctatt cggaggcatt gacacaggca cgggaacagc ttcatgcctg
    18961 atatgcaatc ttcattttcc catcttcggg ctgtgttttt tgattttgac ggcgttattc
    19021 ttgatacgga aacgatgctt tacaaatcct ggtgccatat tgccggattg tacggcgttt
    19081 cctttcctct taaagaatgg gcagccaatg ccggcggata ccagtaccat atttttgacc
    19141 ctgtcacgca tattgaaacg gaagcaggca tcacactgaa ccgaacagaa gtgaacgacc
    19201 ggcgacgcgc ctggtttttg gaaaaagtgg cagcgctgcc cgaagccccc ggcattcgtg
    19261 agtctttgga gtcggctgca tccatggggc tccctctggc tgtggtatcc agttccggac
    19321 aggaatgggt ggaaggcaat ctggagcggc tcggtctgct ttctcttttt gaaaccatct
    19381 gctgcggcag cgaagttgat gccgtcaaac ctgcgccgga tgtctatcag ctggcgttgc
    19441 agcgtctgaa tctgacccct gggcaggtct gtacattgga agattcacca cagggaattg
    19501 ctgcagccgt cgccgctggg atatactgca tagcggttcc caatcaggtg acccgcgctg
    19561 ccgcattaaa agggtaccaa cgacggatcg acagcctgac agaaagaccc ttcaccgaat
    19621 tactactgga cattgagaaa gatctcagcc gtgcgctttg aaagctttgt tgccgcccgc
    19681 tacctgcggg gaaaacgcag aacacgattt atcagcctga tcaccatcat ttccattgcc
    19741 ggagtcagtg tgggggtcac agcgctgatc gtggtcatga gtgtaatgac aggcttcgat
    19801 aatgcgttgc gggaaacaat tatcggaaac cgtgcccacc tcactgttgc caatccgatg
    19861 gggcggccca tggagaatgt ggaggaagta ataggagatc ttatggacct gagccccgag
    19921 attctcggag cagggccgat tattcaggct gaggctctcc tgaaacgcaa caaatataca
    19981 actggcgctt ttctgctggg tatagatccc gaaaaagagc agggtgtcac agaccttgca
    20041 cagaacctga caagaaacgg cgggcgaacc ttcggcgaag gcgacatgcc cggcgacaag
    20101 gaaattgtgc tggggttccg gctggcacac cgtttggcgg cgcgggtggg aagcgaaatt
    20161 gccgtcatta cggacaaacc aacagtgacc cctttcggaa tgagaccggg caatcaggtt
    20221 ttcctcaagg taagcggtat ttcgcaggca aaaatgtcgg atttcgacaa cctgtatgct
    20281 tttgtcaata ttgaaacggc gcgtatgctg acaggacgcc cgggggtgga cggcatacat
    20341 cttcgtctgt ccgatgcctt catcgcagaa cccgtagccc ggctcattga agaacgcctt
    20401 ccctatcgcg ctgaaacctg gtaccagagt caggaagcat tttttgaagc gctgaagcag
    20461 gaaaaagtgg tcatgttcat tattctggtg tttattgttc tggtcgccgc ttttaacatc
    20521 accagcacgc tcatcatgat tgtcatggaa aaacgccgcg acatcggtat cctccgcacc
    20581 ctgggttcca gcagccgttc catcctcacc atttttatcc tcgaggggct catgatcgga
    20641 ttaagcggca ctctgatcgg tcttattctg ggcgtacttc ttgcctacaa cattaatgcc
    20701 gtagccgaat ttattgccgg actgctgggt atggacctct tcaacagcac gatctactat
    20761 tttgacggga tcccggtggc aattgtcgcc tgggatatca cctggattac gctgtcggca
    20821 attgtcctca cctttatctc caccctttat cccgcctgga gtgcttcccg gctcaatcct
    20881 gtggatgcgc tgcgctatga ataatcaagt ccaccctatc cttgcctgcc gcaacctccg
    20941 gaaatgttac cgggatggca gtcgcgaact ggtcatcctc gatgatattt cgctggaaat
    21001 accggaaggg gaaattgttg ccatcagtgg cccttccggc gtgggcaaaa gcaccctgct
    21061 gcatatcatg ggcactctgg atcagcccac atcaggtcag gtttacttta aaggagataa
    21121 cctggcagac atgagtcagg caaagatcaa caggctgcgc aatcagcaca taggatttgt
    21181 ttttcagttc taccatctgc ttccggaatt caccgccctt gaaaatgtaa tgatgcctgc
    21241 gctcagtcag ggaatgcata ggcgaaagtg ccaggagccg gcagcgcatc tcctcgaaca
    21301 ggtcgggtta tcagatcgta tgacccataa gccgggacag ctgagcggcg gcgaacagca
    21361 gcgtgtcgcc attgcccgtg ccctttttaa caaacccgaa ttactcctat gtgacgaacc
    21421 taccggaaat ctcgacgagt caacgggaga aggaattatc gaacttctct ggacgctgaa
    21481 tcagtccagt cacgtttcct ttgtagtggt cacccatgat gaaactgtgg cacagcgggc
    21541 aggtcgctgg atagcccttc atgatggaaa agcttttaca cgcaatggat gatcccagcg
    21601 ggaacgttgg aatgaggtga cgccatgccg gaagatcggc cttcaaaaga tactgcagtc
    21661 ccggaaaccc cgagaacatt tactcttcgc aaaaccatca tttactccct gcttccgacg
    21721 tttctttttc tgggtggtct ggaatcatgt gcacggctct atgaattatg gcacccgcct
    21781 ctgactctgg attgcggctg gggattcaat gaagacagtc gcatttttat tcctgcgggc
    21841 atccagcgca acaaaatgac cacccgcccg gaaaaagtca tttcttttgt caaacagagt
    21901 tttttaatgc ccaagcctgc cggtacctac cggatgatca ttctcggcgg ttccaatgta
    21961 aattatatgc agatgaacct taaagtcatg tgcaagaggc tggaacagta ccctggagaa
    22021 acgcggcatt ttgagattat taattgcggc ggtctcgctt acggctccga gcgccttcgg
    22081 attatgacac aggaattgct gcagtatacg cccgacctga ttctcattta cgcgggacat
    22141 aacgagtttg aagagttaat ccacaaaaat ctgacaaacc tgaatgcact gcccatccaa
    22201 aagaccgcct attctcttgc catgctcaga accttgcgcg actttgttgc aacggtacag
    22261 ctgacttttc tggatcccca gaaactccgt gaaacacttc caccggaagt ggactggagt
    22321 acaggcactt acgaattttc tcaggaagaa ctcagtgcgc atatggaaag atacagagag
    22381 aatctggacg ctattgtcag caccttccgc aacagcaata tccctgtgct gataagcaca
    22441 gttgccacca actaccggga gccgggcctc catccggctc agaaaatcat ccaacaggaa
    22501 atagccgacc tgtatgctca ggggcaatac gaagcggggc tcacgctggc aagagaaacc
    22561 ctcagcagat ctgcacggca tcaggcatcg gacgttgaaa acggcatcat tcgtgacatt
    22621 gctgaaaagc atcatttacc gctcattgaa ggtgagaagc tgatcatgga ggctgagcct
    22681 catggtgttc ccggcgagac ccttttcagt gacagatgcc acatcacgga tacaggcaga
    22741 aatattatcg tagcggcatt tgaaaaagaa attcacataa ttgcagcgca ccctgattct
    22801 tcgaagtagt gctgcttcaa cgtctgagaa agctctttca tgcttcgatg gtttaaaaaa
    22861 actcttttta tctttctctg tttcggattg ctggcagttg tcctgttctt tttccttgaa
    22921 gtcgcagcac gcctttatca gtctcatatt gaaaaacaca atcccttcat tatcgccgca
    22981 aataagggag aagaactgcc ctatcgggaa atgcggacag aaaacgactt tcagtcgctc
    23041 ctctcttcaa aaatgcaggg aagcgaccgg aagaatgaaa atgaaccgaa actccacccc
    23101 gatacccgca tccttctgaa tgactggctt gactccaaag gtttttcccc tgaaaagcgg
    23161 atgaagctcc gggaggactt tcgctcctgg tccacacaga cacgggacta ttacgcctgc
    23221 ctcacccacg aagcggtcat cgtctttaat gccgagaaca ccatccgaaa ctattttggc
    23281 tccgatgctt cttattttga aggctaccag cggtcaatta cgctggcata tctgcaccag
    23341 accggaattc tcggacggat ttatgaagcc ctaaaggaag tgcggcacac gaacaagaca
    23401 gttaccttga ctctcccctg gtccgagcat attcccggaa gcccggtttt tactgctta
//
LOCUS       DAOPWB010000042        22932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2240_length_22932_cov_5.552957, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000042 DAOPWB010000000
VERSION     DAOPWB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 22932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22932
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2240_length_22932_cov_5.552957"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..4053)
                     /locus_tag="PKN92_05675"
     CDS             complement(205..4053)
                     /locus_tag="PKN92_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48316.1"
                     /translation="MHPSEIEKNIENAVNLCKQERHSEALALLELIRPHIPNSPVLLH
                     MRIEALMKLDRTAEALHDCERLFQRLEQIRTAGDQLKPLFQDGQAEGLSAYVSSQTDQ
                     TNELKALLDQRKSDALDKETLQTTVSKLEARLQQLLAQQEKAAHEQTELLDELTNLRQ
                     KERSTSAALEKAYIDLDEMKESLQAREAEVAEAESELAEKARHVSQLESEVRTLQERS
                     DSAVHTEALLNQELAKLREQGDAKSEELEKARNELSQLQASLSDREAAIQEAQDNNRR
                     HEEAMEAMRQEMSAIYAEADKASEARETLKKELDQLRAAEQSKSEALEAARSQLSELQ
                     NALSDREQSVAEAEKRARENELEIERVREQLNNTLRQAEEAERSEAALAEEVAQLRQN
                     EAAKAAALEQTRQEMGALREDLEKREALLAEETQKREMSSEELRALRQERDEMAKRAA
                     SFAESEQQLAQELEHLRKNEEEKSRVLDGARSEMDTLRSQMAKQQEAVLSAEQISSQQ
                     DAIVQALQKELEALKLQATTASTSEQELSREVQHLRENESSKTSALAAAQKEMDALRQ
                     QLNLREKDAQEAESLNAQKQQELERLRNELNDLRTKTAETLDIESVLAGEMEKLRQNE
                     ASKNEELEKSRQELEDLKRVLKTREEDAAKASSEQEESRATIQQLENELAALRHQNLE
                     QQSSQQALQVELTQIHNEAERQGILQASPQAAQLKKIQEQLLALSVAQEDAQQTIRLQ
                     ASQQVLDEPSQRQTSLQAAASAEGEGQSDGNHLFALNYTMGSSSQRSTQQEAMRVAQQ
                     PSDRQTQQKAADIFTGLETTTSGAGKPAAGGITPIQPGRPAPIQTPLHSEAGIPGLSP
                     VNPPRRPHSDSDDIDSDFEEMYPKPRDVSFVFPDQSVSIAVPRRRSSTPVIFAAIGLL
                     IIAIVGIAGFVIFRAAPKTAGAPAAPASPAAAPSDEVKMLTMEFPADRVFGTLYDPSS
                     QPTSDADWTPYAPAQGVVEYAEGTRFHLVVRKDQVHDLSPLVPLPDKCIASLWLPDID
                     MDPVNLNYLQNLKGLDILYIDQELTVEQKENILKGFKRSVTFNCKKPGSIARDMTPPE
                     ERVLEFSEDYTIGRVAIRQWNEAQAPWQFLEMARGKVTVPAKMEIQIEINQAEKNLEV
                     LNTLDEMAVHTLVLKGETITDSCLDAVAHLRGLLALELIDTAVTDEGLQKIITIRGLQ
                     QIKLHGIAITDAGVKALREFTQLARIDIFDAPGITAESLPAFRSIMALRRLNLEKTSV
                     PIEALRQFAREMSSCSITPN"
     gene            4334..5896
                     /locus_tag="PKN92_05680"
     CDS             4334..5896
                     /locus_tag="PKN92_05680"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNZ48317.1"
                     /translation="MSTEKPEIIKCECGQRMRLPADSQSKYFRCVKCGSFVDRQGVRK
                     KDTQKIRRSDLKLSSARGLQRSALLGIFVDGGLISPQQGKEVSAVAKESRKKTFQAII
                     DLGFATREQLHAFLARESGAASISLSHFSIEQTVAALIPKDMVDTHMVLPIDKLGKSL
                     TVAMVCPMDTKAVQELEEFTSLRIRPMLCTWDDFEAACAKLYPESKTTRPELRLIKPA
                     ESDTPQEHAPVPFKVTKATVPEDTADEEEAELPDTAFALSDKLRQLKTLEIPARIMNR
                     IDSVPGGDKDGLKKISQIIANSPPMTARIISTANSTAYGMSGKVDNVPVAVALLGAET
                     IALVATGTPKQSLQGERDWYQLTRYTRHVAKISAALAAFTEKALPNVAYCAGLLHSIG
                     SFALGAAAPDLYSKIDAALTGSAREEAEKTTLGMGHTEAGWNLAQAWNLPEIITSSIT
                     HYLAPENSPDFQDLAYIVHLATALGSADGVLDITRLDHLGRPLDYLKASGQDVAQALS
                     KSPYLAAAEPAK"
     gene            6076..8331
                     /locus_tag="PKN92_05685"
     CDS             6076..8331
                     /locus_tag="PKN92_05685"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNZ48318.1"
                     /translation="MRYSFVFSAIALFLAVPAVAAPPAVPDTPFIQEYRDFFTLDGRA
                     GDVRALAVDGKNRVWAATAAGIYALKDGKTFTAMQTEEEAGPSFSAAAAPDGRVWIGA
                     WNGLYHTTDKGTLEKVPAIQEPIGAVAASRSCIAAQGPQGLWLNRHGIWTKHTGTWAN
                     SAAALAFESDEIFWSATAHGVYRFNHAVPVRHLYSGDELLASEETAVAVAGDGTIWCG
                     GMGGIDVYKDGSRIQSYTSREGLPNSDVRALAFHPDGTLWVCTALGAARLKDNQWSLR
                     HGMRWLPSDDTRAVAFDKDGTAWVATAKGISAIRRRTMTLAEKADYYYDILLKRKVRD
                     PWIVGISRLLTPGDVESSVHEDEDNDGEFTNHYMVMELFRHLVTGDPVALERAWKARD
                     TMEQFQTITGTSGFIARTFVPVEWDSPENINPNRFHDRNRSYTAREIAEKQIRDPRMK
                     CVEERWRLSADGKHLWKGDTSSDEMCGHFFGYFFFHEYGAKTEAEKKQVGDLAGRVMD
                     YLMEGGFNWKDIDGESTRWGVWSPEHLLKNGDWRSERAINATELLAYLKITAHVTGNP
                     KYEEAYRQLINDHGYLEYARAPKSMDPSDHTEIDTSLLVLILPALMTLEKDEAIREVY
                     KEGLYQLFNQTKDQASPFYNFICAAYGVEDFDPEPCVKYLRDSPLDLIMWTVDQRQRE
                     DLNLVRWPELDKWQVDRLLPADERFTMRWDKNPYEAVAGDGGHRESTGVHWLLPYWMG
                     RYFGFIEGAAD"
     gene            8403..9821
                     /locus_tag="PKN92_05690"
     CDS             8403..9821
                     /locus_tag="PKN92_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48319.1"
                     /translation="MKSVLPLFMLCILTSFFIQAAEVSIDDDGMFVINGERSFVLGLY
                     ENPKEDEQLARMVEAGFNLVQAQPKAEELDRLDRHGVYGWVNTGSCIDLSVNREQREK
                     ELTALVGTLKDHPRLLVWEVPDEALWNCWHQPQQWRWHQEPEALRQKIKEVADDDKRA
                     QLFAEVNAALAYRDIADYETWEKRMDMLWEALGLEQPHPELSMARAQERAELMCRGMI
                     DGYHLTRKMAPGIPVWMNHAPRNQISQLAAFNEGADIVGCDIYPVPRHPAISHSDLDN
                     ALISSVADYTVRMQAAAPWKPVWMVLQGFGWGDIQPNQSEQVRKELRRPNFGESRFMA
                     YDAIVRGAKGILYWGTAYVEKESEFLTELLRVIRELHELQPVLSAPRAALDITVSYRP
                     TFGSVDRKPVVLPKAYGKIPWLITVNEWSEGLACTLSGFGNLEGVRYRDHHTGETYQV
                     TDGVLPVPFRRYSARVLEPVVE"
     gene            9984..10496
                     /locus_tag="PKN92_05695"
     CDS             9984..10496
                     /locus_tag="PKN92_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNZ48320.1"
                     /translation="MKAELQKAFNAQINEELFSAYLYVSMVNYFEEQNLKGFASWMQA
                     QAVEELTHAQKLIAYLHERGVAVKLEAIGKPEATWKSPLAAFEAAYKHECYISDCINK
                     LYALATKSGDAAAAIFLSWFITEQVEEEASVDEVVQTLKLAQGAPGALFMLNRELGAR
                     SPGITIAPSA"
     gene            complement(10578..11168)
                     /locus_tag="PKN92_05700"
     CDS             complement(10578..11168)
                     /locus_tag="PKN92_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNZ48321.1"
                     /translation="MSFIKAQCGAEEPAKESPLLAQLLQSRIVTIVGEVDQELAEEVI
                     STLLLLDARSQDLIKVIVSSPGGHVDSGYAIHDMMRFIKSPVAAIGAGWVASIAVPIL
                     LGADKDKRFSLPNTRFLLHQPSGGAGGQASDIRIEAQEILKIRTRINQLISDETGRKV
                     EEVAKDSDRNFWMNAKEALDYGLICRIISNADDITS"
     gene            complement(11223..11981)
                     /locus_tag="PKN92_05705"
     CDS             complement(11223..11981)
                     /locus_tag="PKN92_05705"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ48322.1"
                     /translation="MRLKNKVAIITGAGLGMGREASVLFAEEGAKIIGLDVNAAALEE
                     TAALVKAAGSEMLAVAGDVSKEADVKNAIAQGVARFGKLDILYANAGVLWKDRDFSVL
                     DTTEENWDIVQAINLKGPFFLTKHGIPELKKSGGGSIILIGSISALAGFDLAQDSYTC
                     AKGALISLTKSLAVQFGPDKIRANIIHPGMIDTPLQAPYLNDEKKKNIASGIPIRRLG
                     VARDIAYAALYLASDESTFCTGSELVVDGGFYAM"
     gene            complement(12086..13903)
                     /locus_tag="PKN92_05710"
     CDS             complement(12086..13903)
                     /locus_tag="PKN92_05710"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNZ48323.1"
                     /translation="MTAYILVEGGGSGTRAALVNEKGQREKAYERGPSNPVAYGIVQA
                     AQVIVSLAKEALAGRSAESCVLYAALAGAAEKEVQAALAQRIGTALQLRRIFICPDLH
                     AMLLANAGAEEGILVISGTGASVLAKDRQNRLLKVGGWGAVLGDEGSAYNLAVSALRT
                     AARAEDQIGQSTILAENLTEAAGLPEFSAFSRWISSATKREVAALAPAVLTAAEQGDL
                     PALQCVEEEAWQLALRVCAAHEQLRLRPDAAVYEYGGLLEGSMLFRQYFHQSLDSLPE
                     LKCQPCACRGLDAVSYLHTLEKCPDWASEWTMEPGAAKVLALPDTERAGKGKTLDELT
                     PEEIVAAMQHADMEALQAVGAASESIAAAVDYAGHCLKQGGRLIYTGAGTSGRLGVLD
                     ASECPPTFGVSPDRVVGLIAGGDRALRDSVEGAEDDLVLGAADLEALNPDGRDFVIGI
                     AASGTTPYVAGALSAAARAGARTALITSSTASPIPSDYPVVLNTGPEVLTGSTRLKAG
                     TAAKLVLNRISTGAMAQAGLIYKGRMVGMAPANIKLRHRAERIVRELTGLTQEESAVL
                     LNQADGRITAAVVMGIKQVPLSEAETLLEQHGNRLREIL"
     gene            complement(13900..15168)
                     /locus_tag="PKN92_05715"
     CDS             complement(13900..15168)
                     /locus_tag="PKN92_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNZ48324.1"
                     /translation="MKPFPSDFLFGVSIGAHQCEGGDDGSDWYRWALRPGHILDGSDQ
                     NPGSGHWLHFRDDLALAHKLGMNAVCIGVNWARIQPAPDCFDAEALEHYRQVFQLASS
                     LDMTVLGVLYHTALPRWIADRGGWQAPECPDHFEAYTRETVRMLGEWCLHWIPVQEAE
                     CLVTRLCREKKWPGSGLGVRHEAVLRNTLAEAHVRAAEAIRESISQAVIGLSVRGIPV
                     EPWDEHSPWDMRLAEKEQERLNRAFPEAVFRASGSDGADFIALSYQGVLRTRFAAGRF
                     RYGFSLPVDQDGRAVDFDELQPSSDGLEEVLLLLSAFQKPIAVTGLGFAADDDRERCR
                     FLCDHAGRLLTLARREEPSLKIQGLFYSPFLDGFEWHHGCARRCGLVHVKRPGLERTP
                     NPSAWLWKDIAQHQEVRPGTAARYCGEEEI"
     gene            15316..15549
                     /locus_tag="PKN92_05720"
     CDS             15316..15549
                     /locus_tag="PKN92_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48325.1"
                     /translation="MSHCTPEEFIVSYGTYSTTDKRCATCRFWEGPRQITFNKDKPFR
                     VKATSGSYKCAAFPSKSTSAVNRCQRFMPWEKL"
     gene            complement(15584..19006)
                     /locus_tag="PKN92_05725"
     CDS             complement(15584..19006)
                     /locus_tag="PKN92_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ48326.1"
                     /translation="MAHKECFVSLLLLALLLFPFGTADASEPEAVWSYDTGAKIYAPP
                     LAADLTGDGRCEIVVPASRARRLLCFNGIGEVLWEFALDDDNTDGFHAAVSAVDYDGD
                     GQKELFFVTRGGTAGCLDASGHLIWRVCLGDQMDYTGVLAADINGDGRVELLFGSESG
                     TLYCLSDGGEVLWRHQRRGAIRGIPAVLRFYAGGPLRVVAVWSGGDEACLDGDGNVIW
                     AFSEPAAAGERRSGAAAGDLEGKGTMTVLTATEDYHLIARDGESGSEKWRFKGNGRID
                     QTCSFALADFDGGGRLDIVGGDSSGCVYRIRDGAALWTANTGGGIVQGAAVGDADGDG
                     IQDVLVCSRSGRLLSLNGTSGDIQWSFETAAAPLTTPVLADVDGDGKLEILFTAKDQH
                     LYCLRAGGSAKGAETDWPMINKDLQLTGNARGDALFTGEPAVERPDFFPPPLSVPEEV
                     TLHFGRNRISFEFTNDSYRARHLEVRADLVFPDGQTAVRLFSTFCDPYSREERTLSFD
                     CFDSGRYGIKWGLSDLGTGERLATISRIFSFTALQEEDARLQRLQEEGERRLAQIQDT
                     ALAQRARSAFDDALLPAQKACEAAHQGMRVNPESVAAAHSALALLEKTVAPLRVVPEG
                     KEQVLFGAAAVSSLVKVFPDTAFPPLPEEGEVARTLSMHLACNEYESAQLVVVPLWAE
                     LPGLTLRPGTLMHTAGAGVIDASQIALHRVGYVEIGLPEYSFPVERTGCYPDPLLPPD
                     AFTVPAEQTAQPYYVTVHTLPDSAPGLYEGSITVEAEGAAALQVPLKVWVWDFEIPKE
                     NSLTTSFWMNENYIRQFYKYPDRLPEEIRRRYYDLHLEYRVAPLKALPLGGGDLADDM
                     DYVLKHGQQQFFVSLPILNTEESRADYQKKIRDTQELIRQRGWTADVFFYTHDEVAVV
                     GRHLIPEVVTVNRWAKETFPEWPRLQTSAPEQSLVGSVDIWCPTIDHFNPAFLKRRMA
                     AGERLWLYTVWERPGIMIEFPNTDYRIMFWQCWKYGAEGFLYWGTTHWEYNVQGEERW
                     PAVPWITYNSQPGHNGCGYLVYPGTGGTPLPSLRLALVRDGIEDYEYLHLLEKRFEEK
                     KSSLTAAERDEVHSLLAVTPAVIQDHQHYTDDPAVLSLEREKLARWIERLGR"
     gene            complement(19085..19357)
                     /locus_tag="PKN92_05730"
     CDS             complement(19085..19357)
                     /locus_tag="PKN92_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48327.1"
                     /translation="MVRRGECVKEINVAGIEPAESEVIENAHPDDENGDGVITEQEAA
                     DALSRWQNEGGSMSLALRAYYIHLSGGKYWYDSSAFGSECWRPMTE"
     gene            complement(19487..20365)
                     /locus_tag="PKN92_05735"
     CDS             complement(19487..20365)
                     /locus_tag="PKN92_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ48328.1"
                     /translation="MTRSFDPSLMSRRTFLHTASAAAAGAMLFSSTACASAQMPLAGR
                     IKKAIVYNMFQSDAPVVEKFRALKEAGFQGVEAQTKAPEDPAEMVAAARETGVEIHSL
                     MHGSVDNIPSAVDRAKALGVNVILIVAGRVNEQKSYQDNYTETQAVIREALPYAAANE
                     VTLLVENVWNNFLLSPLEMARYIDEFESPWIQSYFDVGNVVRFAWPEHWIPVLGSRIK
                     RVHVKEYSRDKQMNEGLWKGFDVEMGEGSIDWELVRKELLAIDYTGWVTAEVGGGDIE
                     RMKEISRQMDSILALC"
     gene            complement(20395..21645)
                     /locus_tag="PKN92_05740"
     CDS             complement(20395..21645)
                     /locus_tag="PKN92_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48329.1"
                     /translation="MSNFKLGFIGAGFIANFQAKAVAQIRGVDITGVYSLKGAEEFVA
                     NVKALGVGDCAIYSSIDELCRNVDAVAIFAPNYVRIDIMKEIAVSVKAGAALKGIICE
                     KPLGRTVAEARQLVALAKEAGIPTAYFENQIHMKGIRAQMQQLAPVQAQMGPLALSRS
                     AEEHCGPHEPWFWNPIQQGGGVLSDMGCHSIAVGWFVLTPTGKPITFLKPVAVSAVTS
                     LLKWGVPKYRQELLDRTGVDYSKTPAEDFATGMVTFQNPETGQLVQAQFTNSWMYDKQ
                     GLRLLMDGLGPGYAFELNTLRSSLEVFTGDQAAESVADSETALEKSTASRGLLTVETN
                     EPDLYGYVDEIKDAVACFQEGKDGFLNFEYGFEITRLCQAAYMSAERGVTIDLTDPKI
                     QEELESYVSLIAQGKGADLLYGTK"
     gene            complement(21825..>22932)
                     /locus_tag="PKN92_05745"
     CDS             complement(21825..>22932)
                     /locus_tag="PKN92_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ48330.1"
                     /translation="AFLQEWEQKLAAYIGVPDVAAVSSGRQALRLILEHLGVGAGDEL
                     IVPAYTLGEMLPFVASLGVTLVPADIDPESMNIPPEAVAHRLTGKTRAILALHIFGVP
                     CDIQGIRALADHHGVKVIEDCAHSLGARVQGRPTGSFGDASFFSFEITKMINTYGGGL
                     VASSTRPLVDAARAFNNSEPPGYTTLENKMQGVRLEESFFRRRLMYLPLFMLASPAWQ
                     PLMNRLYRSSQQKQKKREQYSDVQARLGLIKLAHLEERTALRQERIALMNSLLHEDIR
                     PQQVREGDSASGYFYVVRLPVKAAAVRRRLLFRGIDAGAGNEIMDHCAAQLGYTDCPG
                     IDTVFEHALALPMYDGIKEKDCEQVILTLNKVLA"
BASE COUNT         5217 a         6709 c         5891 g         5115 t
ORIGIN      
        1 ctagtcagac caactggttg tgaatcacca tacaaatttc cggcgacagg cgctgcactg
       61 gaagggcctg agagaagagc acaggcaccc gggcaccgtt gctttcctct gtttttgcgc
      121 cagccggaga ggcaatttca gtgtatcaga atatttttcc gttatccaaa cccggtgccg
      181 ggaaagttcc accgcaacgg ctgattaatt gggcgtaata gaacaggaac tcatttcccg
      241 ggcaaactgc cggagcgcct caatgggaac agaagttttc tcaagattca ggcgtcgcag
      301 cgccataatg gagcggaagg ccggcaggct ttccgccgtg attcccggtg catcaaaaat
      361 atcgatgcgc gccagctgcg taaattcgcg tagtgcctta acgcctgcat ccgtaatggc
      421 aataccatgc aattttatct gctgcagccc ccggatggtg atgatttttt gcagtccctc
      481 atcagtgacg gctgtgtcta tcagttcgag agcaagcaat ccgcgcagat gcgcgacagc
      541 atccaggcag ctatccgtaa tggtttcgcc tttgagcacc agcgtatgaa cagccatctc
      601 atccagcgta ttcagcactt ccaaattttt ttccgcctga ttaatttcaa tctggatttc
      661 catcttggcg ggaacagtca cctttcctct cgccatttcc agaaactgcc agggcgcctg
      721 ggcttcattc cactggcgaa tagcaacacg acctatcgta tagtcctccg aaaattccag
      781 aacccgttct tcaggcggtg tcatgtctct ggcgatgctt cccggttttt tgcagttgaa
      841 ggtaacggac cgtttgaagc ccttgaggat gttttccttt tgttccactg ttaattcctg
      901 atcaatatac agaatatcca gtcctttcag gttctgcaaa taattcaggt tcaccggatc
      961 catgtctata tcgggcagcc acaaggaagc aatgcatttg tccggcagcg gcaccagcgg
     1021 cgacagatca tgaacctgat ctttccgtac gaccagatga aagcgtgtgc cctctgcata
     1081 ttcgacgacc ccctgcgcag gcgcataggg tgtccagtcg gcatcgcttg tcggctggga
     1141 ggaagggtca tagagagtgc cgaacacacg atcggccgga aattccatcg tcagcatttt
     1201 aacttcgtcg gaaggggcag cagccggcga tgcaggcgct gccggggcgc ctgctgtttt
     1261 gggagcggca cgaaagataa caaagcctgc aatcccgaca atagcgatga tgagcagccc
     1321 gatcgccgca aaaattaccg gcgttgagct gcgccggcgc ggtacggcaa tagacacgga
     1381 ctggtcgggg aacacaaaag atacgtcccg gggcttggga tacatttctt caaagtcgct
     1441 gtcgatatca tcggaatcgg agtgcggtcg ccgcggagga ttcacaggcg acaatcccgg
     1501 gatccccgcc tcggaatgca gcggggtctg gataggagca ggtctgcccg gctgaatagg
     1561 cgtaatacca cccgccgccg gtttgccagc gccggacgtg gtggtttcca gccctgtaaa
     1621 aatatcggca gccttctgct gggtctggcg gtcagatggc tgttgcgcta cgcgcatcgc
     1681 ttcttgctgc gtcgaacgct gcgaggaaga acccatggtg taattcagcg caaaaaggtg
     1741 attgccatcg ctctggcctt caccctccgc actggcggcc gcctgcagac tggtctgcct
     1801 ttgggacggc tcatccagaa cttgctgtga tgcctggagc cggattgtct gctgggcatc
     1861 ctcctgcgcc acactgagag caagcagctg ctcctgtatt tttttgagct gcgccgcctg
     1921 aggggatgcc tgcagaatac cctgacgctc cgcttcattg tgaatctgcg ttaattccac
     1981 ctgaagagcc tgctgagagg actgctgttc gaggttctga tggcgcagtg ccgccagctc
     2041 gttttccaat tgctggatgg tggcacggct ttcttcctgc tcgctggatg cttttgccgc
     2101 atcctcttca cgggttttca gcacccgttt cagatcctcc aattcctgcc tggatttttc
     2161 cagctcttca tttttgcttg cctcattctg tctgagtttt tccatttcac cggcaagcac
     2221 agattctata tccagcgtct cggcagtctt tgtgcgcaaa tcgttcagtt cattgcgaag
     2281 acgttccaat tcctgctgtt tttgcgcatt cagtgattcc gcttcctgcg catctttttc
     2341 acgcaggttc aactgctgac gcagcgcatc catttccttc tgtgcggcag cgagggcaga
     2401 agtttttgaa ctttcatttt cccggagatg ctgtacctcc cgggaaagct cctgttcaga
     2461 agtcgaggcg gttgttgcct gcagtttaag cgcttccagc tctttctgca atgcctgaac
     2521 aatagcatcc tgctgtgagc ttatctgttc ggcagacagg acagcttcct gctgtttcgc
     2581 catttgcgaa cgcagtgtat ccatttcgct tcgggcgccg tccagcactc tggatttttc
     2641 ttcttcgttt ttccgcagat gctccaattc ctgcgcaagc tgctgttccg attcagcaaa
     2701 ggaagccgcg cgttttgcca tttcgtctct ttcctgacgc agggcgcgca gctcttcgga
     2761 gctcatctct cttttctggg tttcttccgc cagcagggct tcacgtttct ccagatcctc
     2821 acgcaatgca cccatttcct ggcgcgtctg ctccagagcg gccgcttttg cggcttcatt
     2881 ctggcgcagc tgtgccactt cttccgccag agcggcttca gaacgctccg cttcctccgc
     2941 ctgcctcagg gtattgttaa gctgctcccg gacgcgctca atttccaact cattttcacg
     3001 tgcccgcttt tcagcctccg ccaccgattg ctccctgtct gaaagggcat tttgcagttc
     3061 agacagctga gaccgcgccg cttccagcgc ctcgcttttg ctctgttcag cagcacgaag
     3121 ctgatccagc tcctttttca gcgtttcacg ggcttcggat gccttatccg cctcggcata
     3181 aatagcggac atttcctgcc gcatggcttc catggcttct tcgtggcgcc ggttattgtc
     3241 ctgggcctcc tgaatagccg cttcccggtc agacagagac gcctgcaact ggctcagctc
     3301 gttccgcgcc ttttccagct cttccgattt ggcatcgccc tgctcacgca gtttggcgag
     3361 ttcctgattc agcaatgctt ctgtatgcac cgcactgtcg gaacgctcct gcagcgtgcg
     3421 cacttcactt tccagctgag agacatggcg tgccttttcc gcaagttcgg attcagcttc
     3481 cgccacctca gcctcccgcg cctgcaggct ttccttcatt tcatccaggt cgatgtaggc
     3541 tttctccaac gccgcgctag tggaccgttc tttctgtctc agattggtca actcatccag
     3601 aagttctgtc tgctcatggg cggccttttc ctgctgtgcc agcagctgct gaagccgtgc
     3661 ttccagtttt gacacggtgg tctgcaatgt ctctttgtcc aatgcatcag acttgcgctg
     3721 gtcgagcagc gctttcagtt cattggtctg atctgtctga ctggaaacgt aggcactcag
     3781 cccctcggcc tgcccatcct gaaacaaagg ctttagctga tcgcctgccg tgcggatctg
     3841 ctccagacgc tgaaacagtc tttcgcagtc atggagtgct tctgcggttc tgtccagctt
     3901 catgagcgcc tcaatgcgca tatgcaaaag cacgggactg ttcggtatat ggggtcggat
     3961 cagttccaga agtgccaatg cctcggagtg acgctcctgt ttgcacagat tcaccgcatt
     4021 ttcaatattc ttttctattt cgcttggatg catggtatgt tcctgctggg tccataaaac
     4081 acaatatgca tatcatactc aaaagccttc ccgcgggaca aaagtaaaaa gccttacccc
     4141 gcatagaagc aagtgcaagc cttttgcctt tttctttctt gacgcacagg cacactttat
     4201 tttatagtgt tttgacttac agtacaagat tgcgaaaaac cggctgatct gttcttacac
     4261 gaaagagcgc gcggcggctt cctctgcacc ggtgctgttt gtgaactgta gccttttttc
     4321 aggaggtcag acgttgtcaa cagagaaacc cgagatcatc aaatgcgaat gcgggcaacg
     4381 gatgcgtttg cctgccgact cgcaaagcaa atatttccgc tgtgtcaagt gcggttcctt
     4441 tgtagatcga caaggggtac gcaaaaagga cacgcaaaag atcaggcgca gcgacctgaa
     4501 gttgtcatcc gcgcgggggc tgcagcgaag cgctctcctc ggaatctttg ttgacggcgg
     4561 tctgatttcg ccgcagcagg gaaaagaggt gtctgctgtt gctaaagaaa gcagaaaaaa
     4621 gacttttcaa gccattattg atctgggctt tgcaaccaga gagcagctgc acgcattcct
     4681 cgcacgcgaa tcaggagctg cgtccatcag tctgtcacat ttttccattg aacagaccgt
     4741 ggcagcacta attccaaaag atatggtgga cacccatatg gtgttgccta tagacaaact
     4801 gggcaaatca ttgaccgttg ccatggtatg ccccatggat acaaaagccg ttcaggagct
     4861 ggaagaattt acaagtttgc gtatccggcc catgctttgc acctgggatg attttgaagc
     4921 ggcatgcgca aaactgtatc cggaatcgaa aacgacgcgt ccggaattac ggttaataaa
     4981 acctgctgaa tcagatacac ctcaggagca tgcgcctgta ccctttaaag tcaccaaagc
     5041 gacggtgccc gaagacacag ccgatgagga agaagctgaa cttcccgata cagccttcgc
     5101 actctcggac aagctgcgtc agctgaaaac gctggaaatt ccggcgcgca tcatgaatcg
     5161 tattgactcc gtaccgggcg gcgataaaga cgggctcaaa aagatttcgc aaattatcgc
     5221 caattcaccg cccatgactg cacgtatcat cagcacggca aacagcactg cctacggtat
     5281 gtcaggaaaa gtggacaatg tgcccgttgc cgtggctctg cttggcgcgg aaaccattgc
     5341 ccttgttgcc accggaacgc ccaagcagtc tctgcagggc gaacgggact ggtatcagct
     5401 gacacgctac acccggcatg ttgccaaaat cagcgcagcc ctcgccgcct ttacggaaaa
     5461 agcgctgcct aatgtggctt attgtgccgg actgcttcac agcatcggca gttttgcgct
     5521 gggtgccgcc gcccccgatc tttacagtaa aatcgacgct gcgctgacag gatccgcccg
     5581 ggaagaagcc gaaaaaacaa cgctggggat ggggcacacg gaagcaggct ggaatctcgc
     5641 ccaagcctgg aatcttccgg aaatcatcac ctcaagcata acgcactatc tggcgcctga
     5701 aaattcaccg gattttcagg atctcgctta catcgtccat ctggcaacag cgctgggaag
     5761 tgctgatggc gtattggata tcacacgtct ggatcatttg ggacggcccc tcgactatct
     5821 taaagccagc gggcaggatg tcgcccaggc attatccaaa tctccctatc tggcggcggc
     5881 cgaaccagcg aaatagcgga ctcgtccgcc tgcaatccgg cttaagaaaa cagcactgcc
     5941 ttcacacggg cagtgcagtt tctttttata gtttcccggt cagccgtgag tgctgctgga
     6001 caaaacagca acagcatctc accgggctca ggcccggagc agtgccggtg tcaccaagaa
     6061 agtaaggaga acataatgcg ttattcgttc gtcttttccg ccattgccct ttttctggca
     6121 gtgcctgccg ttgccgcacc acctgccgtg ccggacacgc ctttcattca ggaataccgg
     6181 gactttttta ctctggacgg tcgtgccgga gatgtgcgtg ccctcgccgt ggacggcaaa
     6241 aacagagtat gggcggcaac ggctgcagga atttacgccc ttaaagacgg aaagactttc
     6301 accgccatgc agacagaaga agaggcaggt ccttccttca gcgctgctgc agcaccggac
     6361 ggacgggtct ggattggcgc ctggaacgga ctctaccaca ccacggacaa aggtacgctg
     6421 gaaaaagtgc cggctatcca ggaacccatc ggtgctgtcg ctgcgtcccg gagctgcata
     6481 gccgctcagg gaccgcaagg gctttggctc aacaggcacg gcatctggac aaaacacaca
     6541 ggcacctggg caaattctgc agccgctctt gcctttgaat ctgatgaaat cttttggtct
     6601 gccaccgcac acggcgtcta ccgcttcaac catgccgtgc cggtgcgaca cctctattcg
     6661 ggtgatgaac tccttgccag cgaagagacg gcggttgctg ttgccggcga cggcactatc
     6721 tggtgcggag gcatgggcgg catcgatgtg tacaaagatg gcagccgtat ccagtcctat
     6781 acttcccgcg aaggactccc gaacagcgat gtgcgtgccc tcgcctttca tccggacgga
     6841 acgctttggg tctgcactgc tctcggcgct gcaagattga aagacaatca gtggtcgctt
     6901 cgccacggta tgcggtggct cccttctgat gacacccggg ctgtcgcatt tgataaagac
     6961 ggaaccgcct gggtcgccac agccaaagga atcagcgcca tccgccggcg gacgatgacg
     7021 ctggctgaaa aagcggatta ctactatgac attctgttga agcgaaaagt gcgcgacccc
     7081 tggatcgtag ggatcagcag gctgttaacg ccgggagatg ttgagagttc cgtgcacgaa
     7141 gacgaagaca atgacggaga attcaccaac cattatatgg tgatggaact tttcaggcat
     7201 ctggtaaccg gagatcctgt ggcgctggaa cgagcctgga aagcccggga caccatggaa
     7261 cagtttcaga ccattaccgg cacttccggc tttatcgcac gcacctttgt tcctgtggag
     7321 tgggactccc ctgaaaacat taatcccaac agattccatg atcgcaaccg cagttataca
     7381 gcccgtgaaa ttgccgagaa acagattcgc gatccgcgca tgaaatgcgt ggaagaacgg
     7441 tggaggctca gcgccgacgg aaagcatctg tggaaaggcg ataccagctc cgatgaaatg
     7501 tgcggtcatt tcttcggtta ttttttcttt cacgaatacg gcgcaaaaac ggaagcagag
     7561 aaaaaacagg tgggcgatct tgccggaagg gtcatggact atctcatgga aggtggtttc
     7621 aactggaaag acatagacgg agaaagtacc cgatggggtg tctggtcgcc ggagcatctg
     7681 ctgaaaaatg gtgactggcg ttccgagcgt gccatcaatg caacggaact gctcgcgtat
     7741 ctgaaaataa ccgcccatgt taccggaaac ccgaaatatg aagaggcgta ccggcagctc
     7801 atcaatgacc acggttattt ggaatacgcc cgtgcgccaa agtccatgga tccctctgat
     7861 cacacggaaa ttgatacatc gctgctcgtc ctgatcctgc cggcactgat gacactggaa
     7921 aaggatgaag ccatccgtga ggtatacaag gaaggacttt accaactttt taaccagacc
     7981 aaagatcagg cgagtccttt ctacaatttt atttgtgccg cctatggtgt ggaagacttt
     8041 gatccggaac cctgcgtgaa gtatcttcgt gattcgcctc tggatctgat catgtggact
     8101 gttgatcagc gacagcgcga agatctgaac ctggtaagat ggcctgagct ggataaatgg
     8161 caggttgacc ggcttcttcc cgctgatgag cgcttcacca tgcgctggga taaaaaccct
     8221 tatgaagcgg tggcaggcga cggcggtcac cgcgaatcta caggtgtaca ctggctgctg
     8281 ccttattgga tgggtcgtta cttcggcttt attgaaggcg ctgccgactg agtcgccctt
     8341 cagcgaaaac tgttttgaaa ttttttaacc gtaccttttg actttatctc aggagaacaa
     8401 aaatgaaatc cgtgcttcct cttttcatgc tatgcatact tacatccttt tttattcagg
     8461 cagcagaagt atccattgat gatgacggca tgttcgtcat taacggtgag cgcagcttcg
     8521 tgctgggtct gtatgaaaat ccgaaagaag acgagcagct cgcccggatg gtagaagccg
     8581 gattcaatct tgtacaggcg cagcctaaag cggaagaact ggatcgtctc gaccgccatg
     8641 gcgtatacgg ctgggtaaat acaggcagct gcatagatct ttccgtgaac cgggagcagc
     8701 gggaaaaaga actcacagca cttgtcggaa ctctgaaaga ccatccgcgc cttctggtct
     8761 gggaggtacc cgatgaagcg ctgtggaatt gctggcatca gccgcagcag tggcgctggc
     8821 atcaggaacc cgaagccctc cgccagaaaa ttaaggaagt ggcggatgat gacaagcggg
     8881 cacagctctt cgcagaagtc aatgccgcac tggcataccg tgatatagcc gattatgaaa
     8941 cctgggaaaa gcgcatggat atgctttggg aagcactggg actggaacag ccccacccgg
     9001 aattaagcat ggcgcgtgct caggaaaggg cggagcttat gtgccgcggc atgatcgacg
     9061 gatatcatct gaccaggaaa atggcgcccg gtatccccgt atggatgaac catgcacccc
     9121 gaaaccagat cagccagctt gccgctttta atgagggtgc cgacatcgtc ggctgtgata
     9181 tctaccctgt gccgcgccat cccgccatct cccattccga tctggacaat gcgctgatca
     9241 gcagtgttgc ggattacacg gtacgcatgc aggcggcagc accctggaag ccggtttgga
     9301 tggttctcca gggtttcggg tggggtgaca ttcaacccaa ccaatcggaa caggtgcgca
     9361 aagaactaag gcgcccgaac ttcggggaaa gccgtttcat ggcatacgat gccattgtgc
     9421 gcggtgcaaa gggtattctt tactggggca ctgcctatgt ggagaaagag tccgaattcc
     9481 tcacagaact gctgcgggtc ataagagaac tgcacgaact gcagcctgtg ctttccgcac
     9541 cccgcgccgc cctggatatc accgtatcct atcgtccgac tttcggttct gtggacagaa
     9601 agcctgtagt tctgcccaaa gcctacggaa agattccctg gctgatcact gtaaatgaat
     9661 ggagcgaagg tcttgcctgt accctttccg gtttcggcaa tctggaagga gtccgctacc
     9721 gcgatcatca tacaggtgaa acctatcagg taaccgacgg tgtactgccc gtccccttcc
     9781 ggcgctacag tgcgcgtgtg ctggaaccgg tagtggagta agccccttct gaaataaccc
     9841 tctgaaatga cctgccgacg atggtttcca cagcagaaag cagacggaat attcatccgc
     9901 caaatgacgt ttattgggta tataaggacg gggatcaacc cgaagggttc aacaagagta
     9961 caacaacgag gagactgaat gaaatgaaag ctgaactcca gaaagcgttt aacgcacaaa
    10021 tcaacgaaga gttattttcc gcctatctct atgtgtccat ggtcaattat tttgaagagc
    10081 agaatctcaa gggatttgcc tcctggatgc aggcgcaggc cgtggaagaa ctgacccatg
    10141 cccagaaact catcgcttat ctgcatgagc gcggcgttgc agtgaaactg gaagccatcg
    10201 gcaaaccgga agccacgtgg aaaagcccgc ttgccgcttt tgaagccgct tacaagcatg
    10261 agtgctacat ttccgactgc atcaataagc tgtatgccct ggcaaccaag tccggcgatg
    10321 cggcggcagc cattttcctg agctggttca tcacggaaca ggtggaagaa gaagccagcg
    10381 tggatgaagt ggttcagact ctgaaactgg ctcagggcgc cccgggcgca ctcttcatgc
    10441 tcaaccgcga actgggcgca cggagccccg gcatcaccat cgcccccagc gcataagcac
    10501 tgttccctcc tgtaaacgca tacgggctgg agcctgtgaa aagctccagc ccgttttttt
    10561 attccttgct cagaaaatca ggaggtgatg tcatcagcat tggaaataat ccggcagatc
    10621 agaccgtaat ccagcgcttc tttggcattc atccagaaat tgcggtcact gtctttggca
    10681 acttcttcca ctttgcgacc tgtttcatcc gaaatcagct ggttgatccg tgtccggatt
    10741 ttaaggattt cctgcgcctc aatacggata tctgaagcct gaccgcctgc accgccgctg
    10801 ggctgatgaa gcaggaagcg ggtgttggga agagagaagc gtttgtcctt gtctgctccc
    10861 aaaagaatcg gcacggcaat gctcgccacc catccggcac cgatagccgc caccggagat
    10921 ttaatgaaac gcatcatgtc gtgaatggcg taaccggaat ccacatgtcc gccgggagag
    10981 cttacgatca ccttgatcag atcctgcgaa cgggcatcca gaagaagcag tgtgctgatc
    11041 acctcttccg ccagttcctg atccacttct cccacgatgg taacaattct ggactgcagc
    11101 agctgcgcca gaagcggaga ttcttttgcg ggttcttcag cgccgcattg cgcttttata
    11161 aaagacatgg tcatcctttc gaggtatttt attcaggtac cgtaatgcgc ttgatccggc
    11221 gcttacatgg catagaagcc gccatccacc accagttccg aaccggtaca gaaggtagac
    11281 tcatcggaag ccagatacag ggcggcataa gcgatgtccc gggcaacacc gagacgccga
    11341 atcggaattc ccgaagcaat gtttttcttt ttctcatcgt tcagatacgg tgcctgaagc
    11401 ggcgtgtcaa tcatgcccgg atgaatgata ttggcgcgga ttttatcggg accgaattgc
    11461 actgccagag attttgtcag cgaaatcagc gcccctttgg cgcaggtata agaatcctgt
    11521 gccagatcaa agcctgccag cgcggaaatg gatccgatca ggataatgga accgccgccg
    11581 cttttcttca gctcaggaat accatgtttg gtcaggaaaa agggtccctt cagattgatc
    11641 gcctgaacga tatcccagtt ttcttcggtt gtatccagta cggaaaaatc gcgatctttc
    11701 cagagtacac cggcattggc ataaagaata tcgagtttac cgaagcgggc aacaccctga
    11761 gcaatggcat tcttcacatc cgcttctttg gaaacatcac ccgccacggc aagcatttca
    11821 ctgcccgccg ctttcaccaa agcagctgtt tcttccagag cggcggcatt cacatccagc
    11881 ccgataatct tcgccccttc ttcggcaaac agcacagagg cttcacgccc cattcccaga
    11941 ccggctccgg taataatggc aactttattt ttcagacgca taacagatct cctgatgttg
    12001 aggttgggga cacagagcac agaataaaca tagtatatca aagcctttca tgggagtgcg
    12061 acagtgctgc gccggggaac acaaatcaca ggatttcgcg caatctgttt ccatgctgtt
    12121 ccagcagagt ctccgcttcg gataaaggca cctgttttat gcccataacg actgctgccg
    12181 taatcctgcc gtccgcctga ttcaggagca cagcactttc ttcctgtgtc agccctgtca
    12241 gttctctcac aatacgctcc gcccgatgcc gcagtttaat attggcaggc gccataccga
    12301 ccatgcgccc tttgtaaatc agccctgcct gtgccatggc gccggtgctg atccggttca
    12361 gcacgagctt tgcggcggtt cctgctttca gtctggtgga gccggtaagc acttccggac
    12421 ccgtattcaa taccacagga taatcggagg gaatcggcga ggctgtgctg ctggtaatga
    12481 gtgctgtccg ggcaccagcc cgggctgctg ctgacagcgc tccggcaacg tacggagttg
    12541 tgcccgatgc cgcaatgccg atcacaaaat cccgcccgtc cggattcaat gcctccagat
    12601 cggcggcgcc cagcacgagg tcatcctccg ccccctccac actgtcgcga agagcacggt
    12661 cgccgccggc aatcagcccg acgacacgat ccggcgacac accaaaggta ggcgggcatt
    12721 ccgacgcatc cagaaccccc agacgaccgc ttgtcccggc acctgtataa atgagacgcc
    12781 cgccctgttt aaggcaatgt ccggcatagt ccacggcagc agcgatgctt tccgatgcgg
    12841 caccgactgc ctgaagcgct tccatatcgg catgctgcat ggcggcaact atttcttccg
    12901 gagtcaattc atcaagcgtc tttcccttgc ctgccctttc cgtatccgga agggcgagaa
    12961 cctttgctgc accgggttcc atcgtccatt cagatgccca gtcgggacat ttttcaaggg
    13021 tgtgcaaata agacacggca tccagtccgc ggcaggcaca gggctggcat ttcagttccg
    13081 gcagcgagtc cagcgactgg tgaaaatact gcctgaacag catactgcct tccagcaaac
    13141 cgccgtattc atataccgca gcatcggggc ggagccgcag ctgctcatga gcggcacaaa
    13201 cacggagcgc cagctgccag gcttcctctt ccacacactg cagtgcggga agatcgccct
    13261 gctccgccgc tgtcagaaca gcaggcgcca gtgcggcaac ttctcttttc gttgcgctgc
    13321 tgatccatcg ggaaaaagcc gaaaattccg gaagacccgc cgcttctgtc agattctctg
    13381 ccaggatcgt actctgcccg atctgatctt cggcacgggc agcggtacgc aaagcggata
    13441 cagcgagatt ataagcgctc ccctcgtctc ccagcacagc gccccagccg cccaccttca
    13501 gcagccggtt ctgccggtct ttggcaagaa ccgaagcgcc ggtacccgaa atcacaagaa
    13561 tgccttcttc cgcccccgca ttggcaagca gcattgcatg aagatccggg cagataaata
    13621 tacgccgcag ctgcaaggct gttccgattc tctgtgcgag ggctgcctgc acttcctttt
    13681 cagccgcccc ggcaagcgcc gcatagagta cacagctttc ggcagaacgc ccggcaagcg
    13741 cctctttcgc cagcgataca atcacctgtg ccgcctgcac gattccgtag gcaaccggat
    13801 tggaggggcc tctctcgtaa gccttttcgc gctgtccttt ttcattcacc agtgccgcac
    13861 gggtaccgga accgccgcct tccaccagta tataggctgt catatctctt cttctccgca
    13921 ataccgtgcc gccgtaccgg ggcgcacttc ctgatgctgt gcaatgtcct tccacagcca
    13981 tgcgctcgga ttgggcgtcc gttcgagtcc ggggcgcttg acgtgcacca gcccgcagcg
    14041 tctggcacag ccgtgatgcc attcaaatcc atccagaaaa ggggaataaa aaagaccctg
    14101 tattttcaat gaaggctcct ccctccgcgc aagcgtaaga aggcggcccg catggtcgca
    14161 cagaaaccgg cagcgctccc ggtcatcatc cgctgcaaag cccagacctg tgactgcaat
    14221 gggcttttga aaagcggaga ggagcagaag cacttcttcg agtccgtccg aagacggctg
    14281 aagctcatca aaatcaacag cacgtccgtc ctgatccaca gggagtgaaa aaccgtagcg
    14341 gaaacgcccc gcagcaaaac gcgtgcgaag cacaccctga taactcagcg caatgaaatc
    14401 agctccgtca ctgccggatg cacggaagac cgcttccggg aatgccctgt tcaggcgttc
    14461 ctgctctttt tccgccagac gcatatccca gggactatgc tcgtcccagg gttcgacagg
    14521 gataccgcgt acagaaagcc cgatgaccgc ctgcgaaata ctttcccgga tcgcttcggc
    14581 ggcacgcaca tgcgcttctg ccagcgtgtt gcgcaaaaca gcctcatgcc ggacacccag
    14641 ccccgagccc ggccattttt tctcacggca caggcgtgtc accaggcatt cagcctcctg
    14701 gaccggtatc cagtgcaggc accattcacc aagcatccga actgtttccc gtgtgtatgc
    14761 ctcaaaatga tccgggcact ccggagcctg ccagccgccc cgatccgcga tccaccgcgg
    14821 caaagcggta tgatacagaa caccgagcac agtcatatcg agcgaggaag caagctgaaa
    14881 cacctggcgg taatgctcca gtgcttcagc atcaaaacaa tcgggagccg gttgaatacg
    14941 cgcccagttg actccaatgc acacagcatt catcccgagc ttatgcgcca gcgcaagatc
    15001 atcccgaaag tgaagccagt gccccgatcc gggattctgg tcggagccgt ccagaatgtg
    15061 tcccgggcga agcgcccagc gataccagtc gctgccgtca tcaccgcctt cgcattgatg
    15121 ggcaccgatg gaaacgccga aaagaaaatc tgaaggaaag ggcttcatag ggcgccattt
    15181 tgccataaaa aacagagcgg gtcaacagcg ggagcagtgc cgcctttcct tctttcgcat
    15241 ttcaactttt ctccggaccg gtacagattg ttgaattttt tcttcccttt gtatataatg
    15301 aaaagataga gtaccatgag tcactgcact ccggaggaat ttattgtgtc ttacggtacc
    15361 tactcgacaa cggacaaacg ctgcgccacc tgccggttct gggaagggcc ccggcagatc
    15421 accttcaata aagacaagcc cttccgggtc aaggcgacat caggttccta caaatgcgct
    15481 gccttcccga gcaaatccac ctctgccgtg aatcgctgcc agcggttcat gccctgggaa
    15541 aaactgtaac cggtacagcc ggctcaggca ccgttgcttc cggtcagcgc cccagcctct
    15601 ctatccagcg ggcaagtttc tcccgctcaa ggctgagtac tgccggatcg tcggtgtagt
    15661 gctgatgatc ctgtatcacg gcaggcgtga cggcaaggag tgaatgcact tcatcacgtt
    15721 cagcggctgt caggctgctc ttcttttctt cgaaacgttt ttcaagaaga tgcaggtact
    15781 catagtcttc aatgccgtcc cgaaccagcg ccagacgtaa agacggaagc ggcgtgcccc
    15841 cggtaccggg ataaaccaga tacccgcagc cgttatgacc cggctggctg ttataggtga
    15901 tccagggtac cgcaggccag cgctcttccc cctgcacgtt gtattcccaa tgcgtggtgc
    15961 cccagtagag gaacccttcc gcaccgtact tccagcactg ccagaacatg atgcggtagt
    16021 ccgtattggg aaattcaatc atgatgcccg gacgctccca gacggtgtac agccaaagcc
    16081 gttccccggc agccatgcgc cgcttcagaa aggcgggatt gaaatgatca atggtgggac
    16141 accagatatc cacactgccc accagcgact gttcgggcgc actggtctga agccgcggcc
    16201 actcaggaaa cgtttctttt gcccatctgt tgactgtgac cacctccgga atcagatgcc
    16261 gcccgaccac cgccacctca tcatgggtat aaaagaatac atcagccgtc cagccccgct
    16321 gccggatcag ctcctgcgta tcccgtattt ttttctgata gtccgcacgg ctctcttccg
    16381 tattcagaat gggcagcgac acaaaaaact gctgctgccc gtgcttaagc acataatcca
    16441 tatcatccgc cagatcgccg ccgcccagcg gaagggcttt caaaggcgct acccggtact
    16501 ccagatgcag atcatagtac cggcggcgga tctcttccgg cagacgatcc ggatacttgt
    16561 aaaactgacg gatataattt tcattcatcc agaaggaagt agtcaggctg ttttctttcg
    16621 gaatttcaaa atcccagacc cagactttca gaggaacctg aagcgccgct gctccttccg
    16681 cttcaacagt aatgcttcct tcatagagac cgggtgccga gtcgggcaag gtatgaacag
    16741 tcacataata aggctgcgcc gtctgttctg cgggcaccgt gaaagcatcc ggcggcagca
    16801 gcggatcggg ataacagccg gtacgttcca ccggaaaact gtattcagga agtccgatct
    16861 ccacatagcc cacccgatgc agagcaatct gcgacgcatc gataacccct gctccggcag
    16921 tatgcatcag tgtacccggc cggagtgtca gccccggcag ctccgcccag agaggcacca
    16981 ccaccagctg tgccgactca tattcattac atgcaagatg catggaaagc gtccgggcaa
    17041 cctcaccttc ttcaggcagc gggggaaaag cagtgtcagg aaagactttt acaagagacg
    17101 aaacggcagc cgcaccgaaa agcacctgct ctttgccttc aggaaccacc cttagcggtg
    17161 caaccgtctt ctccagcagt gccagggcgg agtgtgccgc agcgacagac tccggattca
    17221 ctctcatccc ctggtgcgcc gcctcacagg ctttttgcgc aggcaacagg gcgtcgtcaa
    17281 aagcggagcg ggcacgctgc gccaatgctg tatcctgaat ctgcgcaagc cgccgctccc
    17341 cctcttcctg aagccgctgc aggcgggcat cttcttcctg aagggccgta aaactgaaaa
    17401 tgcgtgaaat agtcgccagc cgttcgccgg tacccagatc actcaatccc catttgatgc
    17461 cataacgccc gctgtcgaag cagtcaaaag aaagcgtgcg ctcttcgcgg ctgtagggat
    17521 cacaaaaggt gctgaacagc cggacagctg tctgcccgtc gggaaacacc agatccgccc
    17581 ttacttccag atggcgggcc cgataactgt cattcgtgaa ttcaaaagaa atgcggtttc
    17641 tgccgaaatg aagcgttact tcctcgggaa ccgataaagg cggcgggaaa aaatcaggac
    17701 gttccacggc aggctcccct gtgaagaggg catcaccccg ggcattccct gtcagctgca
    17761 gatccttatt gatcatgggc cagtctgtct ctgccccttt tgcagatccg cctgcacgaa
    17821 ggcagtaaag atgctgatct ttggcggtga aaagaatctc cagttttccg tcgccgtcca
    17881 cgtccgccag cacaggcgtg gtcagcggcg cagcggctgt ttcaaaagac cattgaatgt
    17941 cgccgctggt accgttcaaa gacaacagcc tgcccgaccg ggaacagacc agcacatcct
    18001 gaatgccgtc cccgtcggca tcgcccacag ccgccccttg gacaatgccg ccgcctgtat
    18061 tcgctgtcca tagcgccgcc ccgtcacgga tgcgatacac acaacccgag gaatcgccgc
    18121 cgacaatatc gagccgcccg ccgccgtcga agtcagccag agcgaaggag caggtctgat
    18181 caatacggcc attgccttta aaacgccatt tctccgaccc gctctctcca tcccgggcga
    18241 tcaggtgata gtcctctgtg gcggtcagca cggtcatcgt ccccttgcct tcaagatcgc
    18301 ccgccgccgc accggatcgg cgttctcccg ccgcggcagg ttccgagaat gcccagatta
    18361 cattgccgtc gccatcgaga caggcttcat cgcccccgct ccatacagca acaacacgaa
    18421 ggggacctcc agcgtaaaaa cgcagcaccg cagggattcc ccggatggca ccacgccgct
    18481 gatgacgcca gagcacttct cctccatcgc tcagacagta gagcgttccg ctctccgagc
    18541 cgaagagaag ctcaacccgt ccgtccccgt tgatatcggc cgccaggact cctgtgtagt
    18601 ccatctgatc acccagacag acacgccaga taagatgccc cgatgcgtca agacagcctg
    18661 ctgtgccgcc ccgggtcaca aaaaacagtt ccttctgccc gtcgccgtca taatccacgg
    18721 ctgatacggc agcgtgaaag ccgtccgtat tgtcatcatc cagcgcaaat tcccacaaca
    18781 cttcaccgat accgttaaag cagagcagcc gccgggcacg ggacgccgga accacgattt
    18841 cacaacgccc gtcgccagtc aggtcagcag ccagaggcgg tgcataaatc tttgcgcccg
    18901 tgtcataaga ccagaccgcc tccggctccg aagcatcggc agtgccgaac ggaaaaagaa
    18961 gaagtgcaag cagcagcaaa gaaacaaaac actctttatg tgccatggct gaatccccgg
    19021 ttgatgtatt cacagattat aacaccataa cctgcaccgg taagcacttt ttcacggcac
    19081 aacttcactc tgtcatagga cgccagcact ccgatccgaa cgcactgctg tcataccagt
    19141 atttgccgcc tgacagatga atataataag cacgcagcgc cagtgacatc gatccgcctt
    19201 cgttctgcca gcgggaaagc gcatccgctg cttcctgttc ggtgatgact ccgtctccat
    19261 tttcgtcatc cgggtgagca ttttcgatta cctcgctttc ggccggttca atgcctgcga
    19321 cgttgatttc ctttacgcac tcgcccctcc gaaccaatat ttctgattag accgcccgcc
    19381 atatctatta tgagacacca tgattttttt atgcaaagtg atgatataaa aagaagtgcc
    19441 cgccgcttta aacgacgggc acaatataag tcttgggcag gacggctcag cagagtgcga
    19501 gaatggaatc catctgccgg gaaatttctt tcatccgttc aatatcgccg ccacccactt
    19561 ccgctgtcac ccagcctgta tagtcaatgg cgagcagttc tttacggaca agttcccagt
    19621 cgatgcttcc ttcgcccatt tccacatcaa agcctttcca cagcccttca ttcatctgct
    19681 tatcgcggct gtattccttg acgtgaaccc gtttgatccg gctgccgagc accggaatcc
    19741 agtgttcggg ccaggcgaac cgtaccacgt tgcccacatc aaaataactc tgaatccagg
    19801 ggctttcgaa ctcatcgata taacgtgcca tttccagagg actcagaagg aaattgttcc
    19861 atacattttc cacaagcagc gtgacttcgt tggcagccgc atagggcagc gcttcccgga
    19921 tcaccgcctg ggtttccgtg tagttgtcct gatagctctt ctgctcattg actcttccgg
    19981 caacgatcag gatgacattg accccgagcg ctttggcacg gtcgacagcg gaggggatgt
    20041 tgtccaccga tccgtgcata aggctgtgaa tttccacgcc ggtttcacgg gcggccgcca
    20101 ccatttccgc agggtcttca ggggctttgg tctgcgcctc cacgccctga aatcctgctt
    20161 ctttcagggc acggaatttc tccacaacag gagcgtcaga ctggaacata ttgtaaacga
    20221 tcgctttttt aatacgccct gcaagaggca tttgcgccga ggcacatgcc gtagaagaga
    20281 aaagcatggc accagccgca gcggcagagg cagtatgcag aaaagtgcgc cggctcataa
    20341 gagacgggtc aaaagaccgg gtcatggttg cttcctttct tacagattac agtcttattt
    20401 tgtgccgtag agcagatccg cgcctttacc ctgtgcaatc agcgaaacgt aggattcgag
    20461 ttcttcctga atcttcggat cagtaagatc gatagtcacg ccccgttccg ctgacatata
    20521 ggcagcctgg cacagtctgg ttatctcgaa accgtattca aagttcagaa atccgtcctt
    20581 gccttcctgg aaacaggcga cggcgtcttt aatctcatcc acatagccgt acagatcggg
    20641 ctcgttggtc tcaacagtga gaagtccccg gctggcagtg gacttttcca gagccgtttc
    20701 gctgtccgcc acggactccg ccgcctgatc gccggtgaac acttccagag aagaacggag
    20761 ggtattcaac tcgaaagcgt agccaggtcc aagcccgtcc atgagcagac gaagcccctg
    20821 tttatcgtac atccaggagt tggtgaactg cgcctgtacc agctgccccg tttcaggatt
    20881 ctgaaaggtg accatgccgg tggcaaagtc ttctgccggt gttttgctgt aatctacgcc
    20941 ggtgcggtca agcagttcct ggcggtattt cggcacaccc catttcagca gagaggttac
    21001 agcgctgacc gcaacgggct tgaggaaggt aatgggcttt cctgtaggcg tgagcacaaa
    21061 ccagcccacc gcaatggaat ggcagcccat atcgctcagt acaccgccgc cctgctggat
    21121 cgggttccag aaccagggtt catgggggcc gcaatgctct tccgcactgc gcgagagtgc
    21181 cagcggaccc atctgcgcct gtacaggcgc cagctgctgc atctgtgccc ggatgccttt
    21241 catatgaatc tggttttcga aataagcggt aggaatacct gcctcttttg caagtgctac
    21301 cagctgacgt gcttctgcca cggttcttcc gagcggtttt tcacagataa tgcctttcag
    21361 cgcagcgccc gcttttaccg atacggcaat ctctttcatg atgtcaatac gcacatagtt
    21421 gggcgcaaaa atagccacgg catccacatt acggcatagt tcatcgatag agctgtaaat
    21481 ggcacagtct ccgacaccca gcgccttgac gttggcgaca aattcttctg cacctttcag
    21541 cgagtaaacg ccggtaatgt caacgccacg gatctgggcc acagcctttg cctgaaagtt
    21601 ggcgataaaa ccggcaccga taaatcccag tttaaaattg gacatgatgc gtgctccttt
    21661 catgaagaca gggttgacgg ttagaggaaa attgattata tcccatcatc cggcaccaat
    21721 caaaagcggc acggacggat gcccggaagt gcatgttttc gcctcggaaa tccccgtgaa
    21781 atgaccttgc ttcaatccgc ccgtaatctg ccgaacactc cggatcaggc aagtacttta
    21841 ttcaatgtca agatcacctg ttcgcaatct ttttctttga ttccgtcata catcggcagc
    21901 gccagagcat gctcaaaaac cgtatcgatg cccggacagt ccgtgtaacc cagctgggct
    21961 gcacagtggt ccataatctc attgcccgcg cctgcatcga ttccgcggaa gagaagccgc
    22021 cgccgcaccg ccgctgcttt taccggcagg cgcactacat aaaaataacc gctggcactg
    22081 tccccttctc tcacctgctg cggcctgata tcctcatgca gcagggagtt catcagagca
    22141 atacgctcct gccgcagcgc cgtccgttcc tcaagatgtg ccagttttat cagcccgagt
    22201 cgcgcctgca catcagaata ctgctcccgt ttcttctgct tttgctgaga actccggtac
    22261 agcctgttca tgagcggctg ccaggctgga gatgcgagca taaaaagagg cagatacatg
    22321 aggcgccgcc ggaaaaagct ctcttccagg cggacgccct gcatcttgtt ttccaacgtt
    22381 gtataacccg gcggctcact gttgttgaat gcacgtgccg catcaaccag cggccgagtc
    22441 gacgaagcaa ccagcccgcc tccgtatgta ttgatcattt ttgtaatttc aaaactgaaa
    22501 aaagaagcgt cgccgaaaga tcctgtcggt cgtccctgca cccgtgcacc cagtgaatgg
    22561 gcgcaatcct ctatgacctt cacgccatgg tgatctgcca atgcccggat cccctgaata
    22621 tcacagggca ctccgaaaat atggagtgcc agaatcgcac gtgttttccc ggtcaggcga
    22681 tgcgccacag cctcaggcgg aatattcatc gattccgggt cgatatccgc cggcaccagc
    22741 gtcacgccaa gagaagcaac aaaaggcagc atttcaccca gcgtgtaggc tggtacaatc
    22801 agctcatcac cggcgccgac cccgagatgc tcaagaatca gacgaagcgc ctggcgtccg
    22861 gaactgaccg ccgccacatc gggcacgcca atataagcag ccagcttctg ttcccattcc
    22921 tgcaaaaatg cc
//
LOCUS       DAOPWB010000043        21936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2374_length_21936_cov_5.538732, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000043 DAOPWB010000000
VERSION     DAOPWB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 21936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21936
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2374_length_21936_cov_5.538732"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /gene="ftsH"
                     /locus_tag="PKN92_05750"
     CDS             complement(<1..1725)
                     /gene="ftsH"
                     /locus_tag="PKN92_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNZ48331.1"
                     /translation="MNNFLKHISLWIVLLIIVVLAMSNLTKMQSDKRPLGEPDFVTQL
                     SQNNIESVVIKEAGANLKEIKVKFREKVDNNTSFDFKTDNFRDEWRAKLDDQGVPYEF
                     QVESTLWQGLLINLLPMLLIFGIIWFFMFRQMQSGNSKAMSFGKSRARMAGQGEKEIT
                     FKDVAGVDEAKEELEEIIEFLKDPKKFSRLGGRIPKGVLLVGSPGSGKTLLAKAVAGE
                     AAVPFFSISGSDFVEMFVGVGASRVRDLFQQGHKHAPCIIFIDEIDAVGRQRGAGFGG
                     GHDEREQTLNQLLVEMDGFNTNDGVILMAATNRPDVLDQALLRPGRFDRQVVVPNPDI
                     SGRAAILDIHIRNQKIPLATDVDISAIARGTPGFSGADLANLVNESALIAARHNAEAV
                     HRQHMEDAKDKILMGPARRSMILSDKDKLATAYHEAGHTLVARLTPGCDPIHKVTIVP
                     RGMALGVTSSLPEEDRHSLTRSYCLASIRVFMAGRAAEEIVYSEFTSGASNDLKRSTQ
                     LAHKMVCEWGMSDLGPLTFGSNDEVFLGRDFTKMRDFSDETASAVDREIHRICDTCYR
                     EAKELLETN"
     gene            complement(1886..2668)
                     /locus_tag="PKN92_05755"
     CDS             complement(1886..2668)
                     /locus_tag="PKN92_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ48332.1"
                     /translation="MTESKNSLLYSILIPAYNEAENLPPTLEKLAALLRREKIPFELL
                     VVNDNSKDDTALVLERLKTDYPELKVVNNTPPGGLGRAIRCGLRHFKGDVVAIVMADS
                     SDAPEDVVACYRKIEEGYDCVFGSRFRKDSRVTMYPPVKLFCNRIVNHAMRLLFMTRH
                     NDLTNAFKVYRRHVIESISPLQAAHFNITIEMSLSALIRKFRIAEIPISWSGRTWGQS
                     NLRLREMGRRYLCTLLMIWFERILILDDLLRETYPHTDQTEK"
     gene            complement(2665..3732)
                     /locus_tag="PKN92_05760"
     CDS             complement(2665..3732)
                     /locus_tag="PKN92_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ48333.1"
                     /translation="MAVILVTGSAGLIGSESVRYFSGQGHTVIGLDNDMRAYFFGDGA
                     STGKMARDLQEQFPRYIHHNTDIRDYTALSAIFSEYQSEIHAVIHTAAQPSHDWAARE
                     PLTDFGVNAQGTQHLLELTRQYCPDAVFIFTSTNKVYGDRPNSLPLIEKESRWELDPG
                     HPYALHGIDECMSLDQCKHSIFGASKVAADIMTQEYGRYFDMKTMVFRGGCLTGPGHA
                     GAELHGFLAWLMKCAVTKTEYRVFGYKGKQVRDNIHSADLVSCFDEAIRAPRSGEVYN
                     IGGSRFSHCSLLEAVSLCEEISGNPMRVHYEDNNRIGDHIWWISDTRKFQQHYPEWKQ
                     RYDLKSILLEIYNALSQGSSA"
     gene            4031..4753
                     /locus_tag="PKN92_05765"
     CDS             4031..4753
                     /locus_tag="PKN92_05765"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ48334.1"
                     /translation="MKKIIAVNIIVTLVCTLLVAYGAWRFLFSETKPRPQGAPTAAPH
                     GEGWIDLLDAEHQSQWVNITDSLDIFEIRDGILHLFGKAMGKLRYAGFKGRSFSDFEL
                     HLEFKVAPRTNSGVFLRAIENDPVRRGFEVQVLDDYGTVPCRTGTGAIYDMVCPMYNM
                     ALPAGEWNSYDIRCEGREVKVTVNGVKVIDTDFSLLTKPYGKFSLAYAELPLEGLIAL
                     QDHGGEVWYRNIRVHPLNETEP"
     gene            4750..6021
                     /locus_tag="PKN92_05770"
     CDS             4750..6021
                     /locus_tag="PKN92_05770"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNZ48335.1"
                     /translation="MNLLLSQNRIRSRFFHRCTLFASMVGIASAAAAGVFHPPSDDFR
                     YPDGCEPLLVLAAGDEVSTACGEGVELSVSSSTCCLQPPTEARAGWAWFDADRIVSTV
                     SGLQKDKAYFLALYWWDADSQGRLQSVQFRQHDGQPWETVLPSAPAAAFSNDKPVPAM
                     IILPVPQEFAACGSFSFSVGGHAGAGAVLQACAVLSKTPQENQKKVLLITGDDYPGHD
                     WQKTSRVLAEELRKDQRLELSVTESPATVGSPLLSYYDAVVVHFKNYDDRLLLDAAVG
                     EAFQRYVASGKGVVFTHFSCGAFQNWAPFEKIAGRIWNPSLRGHDPYGEFTVRMTGSS
                     HPVVASMHDFTTRDELYTCLEGTVPITVLCDAVSCVDQKVYPMAFVVENTGGRVFHCL
                     LGHDPHAYRNAAVADLYRNAVCWVSGLLENP"
     gene            6156..7943
                     /locus_tag="PKN92_05775"
     CDS             6156..7943
                     /locus_tag="PKN92_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HNZ48336.1"
                     /translation="MMTLLIAIGSFVGFLVAYHTYGRWLARKIFALNPDAATPSHTLA
                     DGKDYVATRPAVLFGHHFSSIAGTGPIVGPAIAVLWGWLPALLWVLLGSIFVGAVHDL
                     GSLVVSMRHKGQSIGDVSGKLISFRCRLLFLSILFFALTLVVAVFGLIIATLFSIYPG
                     SVFSVWFAMPLAVCVGVLIYKMKMPLFFPSAVALSLLYLTVYAGVYYLPLKLDMPLFA
                     GEGTSFFQSLHSSVGLWTVLILCYCFLASVLPVWLLLQPRDYISSLQLYVALFALVAG
                     LIIAAPPIVAPALGSVGEGMPPLMPFLFITIACGAVSGFHSVVSSGTSSKQIDNERDA
                     QFIGYGSMLVEAVLAVLVILSCVAGVGMLIHTDSGTLTGQAAFSHYYGVGWDKMTLAH
                     TIAVFVEGAGNLISSLGIPRDLACGVVAVMIAGFAMTTIDTTTRLNRYVIQEMGNAVQ
                     VKMLNNKFVATLVAVGVSAVLAFLPGPQGPGSGGLLLWPLFGATNQLLAGLALMVVIF
                     YLRWIGKPLFFAIPPLLLMLLMPAWAIVNQLSGFSEKKNYLLVAFGTAILILQLWMVI
                     EAIIAWRSVPERAAREEAVSAEVCPKHEG"
     gene            7933..8532
                     /gene="thpR"
                     /locus_tag="PKN92_05780"
     CDS             7933..8532
                     /gene="thpR"
                     /locus_tag="PKN92_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNZ48337.1"
                     /translation="MKDEPSESKQLRVFAALPLPEQMRAVVHRGSDICRNNLQSVVRW
                     VPPENLHLTLRFYGTITEAQLEVIHNTLSGRLAECTAPFLQLAGINAFPSLKRPSVFY
                     VGVRIIQGDLQHLASICEKAALHAGLPEEKKQFKAHITIGRIKRNAVAPCAEEVLSEP
                     GGEVLSGQEFRADNVVLFNSELSSRGPLYSALRNYRLKS"
     gene            8529..8801
                     /locus_tag="PKN92_05785"
     CDS             8529..8801
                     /locus_tag="PKN92_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNZ48338.1"
                     /translation="MIETAILPPTAFGAALYSAITLYMMALLLRWLAPFLSLNIYARP
                     YVWLNPIIDPYLKLIRNTLPSMGFTDWSPVAGILVLWVIRSFVMSM"
     gene            8816..9421
                     /locus_tag="PKN92_05790"
     CDS             8816..9421
                     /locus_tag="PKN92_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48339.1"
                     /translation="MKAIFTGVFLLALCFFVLASGCIPSSPAPVPVDKPAPAKKEAAA
                     ASETKQHQTAEGISTLNVPLQVQGNATGAISMPLIKLVPQSVENTYKLEGTFPVKGIA
                     IDGALVLQPEGKWYLSGKFTSVYPSFKPGAPVYQPMGILQPREGQGLEVVQTSQEGLI
                     LFAVPMPSPDAEKSGQPEEHPFALTFDASPQTVFSVMITPF"
     gene            9441..10328
                     /gene="rsmA"
                     /locus_tag="PKN92_05795"
     CDS             9441..10328
                     /gene="rsmA"
                     /locus_tag="PKN92_05795"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNZ48340.1"
                     /translation="MTTEHGESPEYPRLKELCKRHNIRFKKQLGQNLLLDDNINTIMV
                     GAAGLTPEDTVIEVGSGLGALTKKLVRQAGRLLCVEIDPTFMPCLEEQFGDNPKVQLF
                     RGDILNHELDKLVAEFLPGAGTLKMVSNLPYYVTTPVLFHFLESPLYFERLVVMTQLE
                     VGHRLTAAVNSEEYGVLSVAARLYAHTDIVHRVPASCFLPKPKVDSCIVRFRCHKESP
                     VPGLEIAEIMKVVRAAFRQRRKTLRNTLTGGNLGLSRDQVMVALAEANIADSRRAQTL
                     DWTDFGRLTGIIRNIKNAS"
     gene            10354..13014
                     /locus_tag="PKN92_05800"
     CDS             10354..13014
                     /locus_tag="PKN92_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ48341.1"
                     /translation="MKAFYCVGTHWDREWYKTFQEFRMWLVECVDKAMDLLENEETFR
                     AFHLDGQTIVVDDYLQIRPESRERFLKLVKERRILIGPWYNLPDEWLLSGESLIRNLM
                     RGFRDCARLGVKPLDYAYTPDQFGHIAALPMIMGGFGFKAGIVWRGAQDENYPAQFLW
                     EAPDGTAMAYCKLSDVGSYGPFDFQVRRPVRADGFTEESFRKHFEKYLESEKARNSVP
                     LVLMLDAIDHQDPDPEMVQIFKELRRLYPHIEFVWSSLEDFGDELIKHKQSYPVRKGE
                     LREPVRDSDRYGHYLIVHTLSSRVDIKKRNDQCSALLEKWCEPVLLMAAMNGIHFPET
                     YLEKSWEYLMKNHPHDSICGCSIEQVHRDMHYRYDQSELIAEGLVRRAVAALGEAAAD
                     EGKWQHITVHNPLPYARKGVYDLALYFPSDYGQTTGHIYHDGLATGERYNKFHLVRSD
                     GQRLTYQHKRIERNLEVPRLNERGREMAAPGDVYHVAVELELPPCGSTSFFIEGTNDA
                     TRTFGSLLTGPLSASNGVIEISLDNQGALSLETPDGQQYSGLLQYEDAGDCGDGWTRG
                     IPVNDLVVRSYGSAVMTSIEENGPLRCTFRVERLLSVPSKIEKGTGFRSEERVDLQII
                     DRITLEKHSPFVKIRTIIRNSAQDHRLRVLFPTDCDASVTFAETPFAVVEREIEIPEE
                     SALWQERVNEEKAFSTFFGVQDKDRGLAVLCPAGLHEYAALDTPRTELALTLFRGFAK
                     TVGTQGETDGQLSGTIELEYALVPLTEKADPRQLIRHVSEMQAIPKHHESREAIPTKS
                     HLYQLTQKAVMTALKRSADGKGGIVRFWNPTEKTVSDGFATTGELSGAWLCNLNEEKE
                     TDIPVSGSTIQITIPAFSLKTICFTWKHET"
     gene            13331..13453
                     /locus_tag="PKN92_05805"
     CDS             13331..13453
                     /locus_tag="PKN92_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48342.1"
                     /translation="MDFTALIGSLVNTAVTFLLDTFLNGLFAFLSDLFFGAPIA"
     gene            complement(13545..15557)
                     /locus_tag="PKN92_05810"
     CDS             complement(13545..15557)
                     /locus_tag="PKN92_05810"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNZ48343.1"
                     /translation="MPKKSLFQYSNSFCLFAFFIMLLSGIASAAQPVDLGTLLEEMAD
                     LSRLTTLGKQAYTTGQFSSYDRRSTDPSIATEENWFANTDRNQHLRVEEQSGRKEWVL
                     MDAAGPGAIVRFWSANPTEGGVVRFYLDHASEPVIEMPLASLLEGKTKPFISPICGER
                     GKGWNSYLPIPYEKHCKVTIDASDIYYIINYRTYVSDTEVKSFTLADTEKYNKELRAV
                     SEAFKHPGNFETAPGATVKAFDVTVEGADSEDCSFDGPSAISGIQCKVTADDLEQALR
                     GCLLKISFDGQAPSVVAPLGDFFGTAPGINPYQSLPSGVLADNTLYSRWIMPFKEKAE
                     VELINFNRFPVTLSLHFFITPHAWTDDTLYFHAKWKGVQDIPTRPMQDWNFMSGTGVG
                     RFCGVLLHITNPVTQWWGEGDEKIYVDGDTFPSFFGTGSEDYFGYAWCDTEVFTHAYH
                     NQIRCDGPANFGQSCVSRFHIMDDIPFRKDFRFDMEIWHWADVLISQSITAYWYAAAG
                     ATDNFPEPSADLLTVPVYPVPEAVKGALEGEKMRAIEVSRGTVDAQSGSWPWSSFLQL
                     WWRGGEPGDHLKLRFPVAEAGNYEVSAVFTKAPDYGIHELAVNGKVIAPSVDLFNAGE
                     VIVAEPVSLGRADFTKGLNFIDVKILGKHPDSTDYMFGLDYILLTP"
     gene            complement(15585..16319)
                     /locus_tag="PKN92_05815"
     CDS             complement(15585..16319)
                     /locus_tag="PKN92_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48344.1"
                     /translation="MEDLLGFLIFIVIAVISVISRLAKEKTTTPDIEETLPRPFPVDE
                     LPEATRRMIYGDESWDIPVATPKGGGRTFVPPAEPRRPAEEHRRMPADEPRTVLPSET
                     RPPVFSEPRQVLAGDPQTARRSLAPQEAPFRPAAGRPSPQPVTLQEVLRPAASPLPAR
                     KHQEPPRRAPRPAAAKSKPKAAPAVVTAAAPKRQETMRPAAAPRAVSFLSEEVLRTFS
                     SQEEIRRALVLREILGPPRAFEEIMF"
     gene            complement(16353..17360)
                     /gene="floA"
                     /locus_tag="PKN92_05820"
     CDS             complement(16353..17360)
                     /gene="floA"
                     /locus_tag="PKN92_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262252.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNZ48345.1"
                     /translation="MDAIMGLITLGIFVVFLVVLVVFISYIRLWLRAWLSQASVGWLQ
                     LIGMSLRKVNAATIVDSRIMAVKAGLNVSTNELETHYLAGGNVVRVVQALIAANKANI
                     DLSFQQATAIDLAGRDVLDAVQTSVRPKVIDCPDPTKGAPTVAAVAMDGIQLKAKARV
                     TVRTNIRRLVGGATEETIVARVGEGIVTTIGSSQSHKKVLENPDSISKTVLARGLDAG
                     TAFEILSIDIADVDVGENIGADLQIKQAEADKQIAQARAEERRAMARAREAEMVASVQ
                     EMRAQVVKAEAQVPLAIAEAFRNGNLGIMDYYRMKNISADTNMRESIAKPGSGTSEEL
                     K"
     gene            complement(17372..17746)
                     /locus_tag="PKN92_05825"
     CDS             complement(17372..17746)
                     /locus_tag="PKN92_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNZ48346.1"
                     /translation="MGVRSYPDYFILIIGGELLGAGVGVILGFVVLTKTRASQLLTLN
                     DAQTQEAGYVSAESDLSLIGKEGIVLSALRPAGTIKIGTQRIDVVSDGVFLDEGTRVV
                     VVQVQGSRVLVEAVAQRSKQPE"
     gene            complement(17866..18165)
                     /locus_tag="PKN92_05830"
     CDS             complement(17866..18165)
                     /locus_tag="PKN92_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48347.1"
                     /translation="MNSNEIIVHMLRQLLKEMEVVSSQGAGYYTCVPFARRFNKLLEQ
                     SRLLPGTDNTLLETFESMSEFDPKDPSDKSNVLLGIRVEISQLITYLECLDRSPS"
     gene            complement(18152..19873)
                     /locus_tag="PKN92_05835"
     CDS             complement(18152..19873)
                     /locus_tag="PKN92_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNZ48348.1"
                     /translation="MPFRTLLLSVGFFFLFASTLFFSYATPSTEEKPVKNILVCPVEG
                     EIRDSVSVLVDRVVTEESKDAEAIVFTIDTFGGRVDSAIDIASSILKARVPTIAYVTG
                     KGAISAGALIAYACDTIIMEPGVNIGASTPVMPGVEMSESMNEKSMSFLRAKYRALGE
                     EKGHNPLIGEAMVDPRIELYYTKSGDGSVTVFKVEQGRVIEKYRSGSFSVLTASLSPG
                     ALSASAAPGFSQDSLKELEKLFRGTHPSTDGFSGSAPQDKKPEPPAEKSHLPASSAPD
                     LLPELPSDARLLSPAGNLLTLTAREAQEVALVTYLAYSVEQGLKQLGYTSFTSVAMKM
                     TLPEKIFSFLTSPLIAGLLLLCGVAGIYIEIKTPGIGLPGLIGVVCLSLYFGSRLIFG
                     LASWLDLLLVIVGIILIIAEIFFIPGFGVTGITGILCLFAGIYLSLMRVPIPTYSWDF
                     DRLRDGGITLLVAGSLFIAFVVLTWKYFPRSSFARGLLLADAQFAEAGYTVQTREEQG
                     AIGQIGITASMLRPAGKGRFGNKTLDIVTEGDFLDPGTPVEIILVEGNRYVVRQRADK
                     ESLHEQQ"
     gene            complement(20071..20472)
                     /gene="rpsI"
                     /locus_tag="PKN92_05840"
     CDS             complement(20071..20472)
                     /gene="rpsI"
                     /locus_tag="PKN92_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNZ48349.1"
                     /translation="MIDKNTNEIVALGRRKRASARVRIKPGTGLFIVNGRPVDEYLAR
                     ETLVQMAELPLVTAGLRDSIDVRARCAGGGLAGQAGALSLGIARALVEKDEELRTTMR
                     GKDLLTRDAREVERKKYGQPGARKRFQFSKR"
     gene            complement(20494..20925)
                     /gene="rplM"
                     /locus_tag="PKN92_05845"
     CDS             complement(20494..20925)
                     /gene="rplM"
                     /locus_tag="PKN92_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNZ48350.1"
                     /translation="MKTYVPTQGAEPKQWYLIDAEGQSIGRVAALAASLLRGKHKPQY
                     TPFLDCGDSVVIINAAKVRVIGSKGRKKVYYRHSGYPGGIKETPLEIMLEKHPTRAME
                     IAIKGMLPGNRLGRKLATQYRVYAGAEHPHEAQNPVVYTVK"
     gene            complement(21091..>21936)
                     /locus_tag="PKN92_05850"
     CDS             complement(21091..>21936)
                     /locus_tag="PKN92_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ48351.1"
                     /translation="GAGILDMLMSPIVSAVCVDRRASALNHLHAFYCIGALATVLFAS
                     ACMHLGISWRILAGAAALVPLALTVLIFLTGFPPLVHPERSRDSILHLVLKPRFIFAL
                     LIIWLIGATENGIIQWLPAFSERGLGFDKSMGGIAVGLFSLFMAGGRLAAPQILRRLG
                     AYGVLFAGAFFCIVCYLAGTLIPAPGIALSFCIFAGLACSFLWPTWLGITADIFPHGG
                     ASMFALLAALGNFGGLVLPWAIGGIAEYSSIRSGLLAGSLAPVALIIFALITLYQDRH
                     NPSSL"
BASE COUNT         5348 a         5421 c         5698 g         5469 t
ORIGIN      
        1 gttcgtttcc agaagttcct tggcttccct gtaacaggtg tcgcagatac ggtgtatttc
       61 acggtcaacg gcggaagcag tttcgtcgct gaaatcccgc atttttgtga aatcgcgccc
      121 gagaaacact tcatcgttgc tgccgaaggt aagagggcct aagtcgctca tgccccattc
      181 gcataccatt ttatgcgcta actgggtgga gcgtttcaga tcattggagg cgccgcttgt
      241 aaattcgctg tatacaattt cttccgcagc acgccccgcc atgaacacac ggatagaagc
      301 cagacagtaa gagcgggtaa ggctgtggcg gtcttcttca ggcagggagc ttgtgacacc
      361 cagagccatg ccgcggggga ctatggtgac tttatggatg ggatcacagc ccggggtgag
      421 ccgcgccaca agcgtatgtc ctgcttcatg gtatgccgtg gcaagtttat ctttgtcgct
      481 taaaatcatg ctgcgtcggg caggccccat gagaattttg tctttcgcat cttccatgtg
      541 ctggcggtgc acggcttctg cattatgacg ggccgcaatt aacgccgact cattcacaag
      601 gtttgccaga tcggcacctg aaaatccagg ggtgccacgg gctatggcgc tgatatcgac
      661 gtcggtggca aggggtatct tctggtttct gatatgaatg tctaaaatag cggcacgacc
      721 ggaaatatcc ggattgggaa cgacaacctg acggtcaaag cgtccgggac gcagtaacgc
      781 ctgatcaaga acatccgggc gattggtggc ggccattaga atcacgccgt cgttggtatt
      841 gaaaccgtcc atttcaacaa gtaattggtt gagtgtctgt tcccgttcgt cgtgcccccc
      901 gccaaagcct gcaccgcgct gacgacccac cgcatcgatt tcatcaataa aaatgataca
      961 gggcgcatgc ttatgcccct gctggaaaag atcgcgaaca cgactcgcac caacgccgac
     1021 gaacatctcg acaaaatcgg atccactgat gctgaaaaac ggcaccgctg cttctccggc
     1081 aaccgcttta gctaaaaggg tttttcccga ccccggcgag cctaccaaaa gcactccttt
     1141 agggatacga ccgcccagtc gggagaactt tttcggatct ttaaggaatt caataatttc
     1201 ttccagttct tccttggctt cgtctacacc ggcaacatcc ttgaaggtaa tttctttttc
     1261 gccctggcct gccatgcgtg cgcggctttt cccgaaagac atggcttttg aattgccact
     1321 ctgcatttgg cgaaacatga aaaaccagat aatgccgaat atcagaagca tggggagcaa
     1381 attgattagc agaccctgcc aaagtgtgct ttcaacctga aattcataag gaacgccctg
     1441 atcatccagt tttgcgcgcc attcgtcccg gaaattatct gttttaaagt caaaagaggt
     1501 gttgttgtcg actttttccc ggaacttgac tttgatttct ttgaggttgg cacctgcttc
     1561 tttaatgacg acggactcaa tgttgttttg cgaaagctgc gtgacgaaat ccggttcgcc
     1621 cagcggccgt ttgtcgctct gcatttttgt caggttggac atggccagga ctacaataat
     1681 cagcagcaca atccacaaag agatatgttt gaggaaatta ttcatgataa aggggatatt
     1741 ttccttgaaa ttcggggagc caactcatcc agccacaaag aaagcggagt tttgcaacgc
     1801 caacgctgaa aattcagcat catagaatta tactaacaat tgacaagacc ttacaagcac
     1861 tcgcagaaat acagacccgg cgtctttatt tctccgtctg atcggtgtgc ggataggttt
     1921 cccgaagcag gtcgtccaga atcaggatcc gctcaaacca gatcatcagc agggtgcata
     1981 gatagcggcg ccccatttcg cgcagtctta gattggactg accccaggtg cgcccgctcc
     2041 aggaaatagg tatctcagca atacgaaatt tccggatcag tgcggaaaga gacatctcaa
     2101 tagtaatatt aaaatgggcc gcctgaagcg ggctgatact ttctattaca tgtcgtcggt
     2161 agactttaaa ggcattggtc agatcattgt gcctggtcat aaatagaaga cgcatcgcat
     2221 gattaacgat gcgattgcag aaaagtttta caggaggata catggtgact ctgctgtctt
     2281 ttcggaagcg ggatccaaaa acacagtcgt aaccttcttc aattttccgg taacaggcga
     2341 ccacatcttc cggcgcatcg gagctgtccg ccatgacaat agcaacaaca tctcctttga
     2401 aatggcgtaa cccgcatcgg atggcgcgtc cgagacctcc gggtggcgta ttattcacca
     2461 ccttcagctc ggggtagtcg gtcttcaggc gttccagaac aagcgctgtg tcgtctttgc
     2521 tgttgtcatt cacaaccaga agttcgaaag ggattttttc ccggcgcagc agtgccgcca
     2581 gcttttccag agtgggagga agattttccg cttcattgta tgcaggaata aggatggagt
     2641 agagcaaact gtttttgctt tccgtcatgc agatgatccc tggctcaagg cattataaat
     2701 ttccagaaga atggatttca gatcataacg ctgtttccat tctggataat gctgttggaa
     2761 cttgcgtgtg tcgctgatcc accagatatg atcgccgatg cgattgttat cctcatagtg
     2821 cacccgcatg ggattgccgc tgatttcttc gcaaagcgac accgcttcaa gcagggaaca
     2881 atggctgaaa cggctgccac cgatattgta gacctctccg cttcgtggtg ccctgatggc
     2941 ttcatcgaag caggatacca gatcagcgct gtggatattg tcccggacct gttttccttt
     3001 atatccgaaa accctatatt ccgtcttcgt cacggcgcat ttcatcagcc acgccagaaa
     3061 accgtgcagc tcggcaccgg catggcccgg acctgtcagg caacccccac gaaagaccat
     3121 ggtcttcatg tcgaagtaac gcccgtattc ctgggtcatg atatccgcag caacctttga
     3181 agcaccaaaa atggaatgtt tacactgatc aaggctcatg cattcgtcga tgccgtgaag
     3241 cgcatagggg tgtcccggat ccagttccca ccggctttct ttttcgatca gaggaaggga
     3301 attagggcgg tcgccataca ctttgttggt actggtgaaa ataaaaaccg catcggggca
     3361 atactggcgg gtgagttcca gcaagtgctg cgtcccctgc gcatttacac cgaaatcggt
     3421 gagaggttcc cgtgccgccc agtcatggct gggctgtgcc gccgtatgaa taacagcgtg
     3481 aatctccgac tggtattccg aaaaaatagc ggacagggct gtataatccc ggatatctgt
     3541 attgtgatga atgtaccggg gaaactgctc ctgcagatct cgtgccatct tgcctgttga
     3601 tgcgccgtcg ccgaaaaaat aagcgcgcat gtcattatcc agaccgataa cagtgtgccc
     3661 ctgtccggaa aaatagcgga cactttctga gccaataagc ccggcggaac cggtgacaag
     3721 aattactgcc ataaccatcc tttatgaatg cccccggtag gaggcacgaa gggcttttac
     3781 atgatccggc tgaaaccgac tgatacacca ttctagcgaa aaataacggg gatgaatcaa
     3841 gccggcttcc gaaataaacc cgtttttttt aaggtgaaac aaacagaaaa taattccggc
     3901 agttcccgta aaataaaaaa gcagtaaaaa caaaactatt tcaaggggtt tccggctctg
     3961 ttgacagcaa ggatggaagt ggcgtacagt gtgaggtgtg ctttaaatgc cttccgaaaa
     4021 gggaaatgcc atgaaaaaaa tcattgctgt gaacattatc gtgaccttgg tgtgcacgtt
     4081 gcttgtcgcc tacggggcat ggcgcttctt attttctgag acaaaaccca ggcctcaagg
     4141 ggcgccaaca gcggcgccgc atggcgaagg gtggattgat ctgctggacg cagagcatca
     4201 gagtcaatgg gtgaatatta ccgattctct ggatattttt gaaatccgag acgggatttt
     4261 gcatcttttc ggcaaagcca tgggtaaact gcgctacgct ggttttaagg gacgcagttt
     4321 ctcggatttt gaactgcatt tggaatttaa agtggcgcct cgtacgaaca gcggcgtgtt
     4381 tctaagagcc attgaaaacg atcctgtccg aagaggcttt gaagtgcagg tgctggatga
     4441 ttatggtact gttccctgca gaaccggtac cggcgcgatc tatgacatgg tttgtcctat
     4501 gtataacatg gcattgcctg caggtgaatg gaattcttat gatattcgct gtgagggcag
     4561 ggaagtgaag gttactgtga atggcgtgaa agtgatcgac acggatttct ctctcctgac
     4621 aaagccttac ggaaaatttt cacttgccta tgccgagctt ccactggagg ggcttatcgc
     4681 actgcaggat catggcggag aagtatggta ccgtaacatt cgtgttcacc ctctgaatga
     4741 aacagaacca tgaaccttct cctcagccaa aacagaatcc gttcccggtt tttccatcgc
     4801 tgtacccttt ttgccagtat ggtcggcatt gcgtcagccg ccgccgccgg agtctttcac
     4861 ccgccatccg acgatttccg gtatcctgac gggtgtgagc cgttgctggt gctggcggca
     4921 ggggatgaag tttcgactgc ctgcggcgaa ggcgtggaac tgtctgtttc ttcctcaacg
     4981 tgctgcctgc agccacccac ggaggcgcgt gccgggtggg cgtggtttga tgccgatagg
     5041 atcgtgagca ctgtctccgg gctacaaaaa gacaaagcgt atttcctcgc gctttactgg
     5101 tgggatgcgg acagccaggg acggcttcag tccgtgcagt tccggcagca tgatggacag
     5161 ccctgggaaa cagtactgcc atctgctcct gccgcggctt tcagtaatga caagccagtg
     5221 ccggctatga tcatactgcc tgttccccaa gaattcgcgg cttgcggatc tttttccttc
     5281 tctgttggcg ggcatgccgg agccggtgcc gtgctgcagg catgcgctgt tctgtccaaa
     5341 acaccgcagg aaaaccaaaa aaaagttctg ctgattacag gtgatgatta cccgggtcat
     5401 gactggcaga aaacttcccg ggttcttgcg gaagaattgc gtaaagacca acgcctggaa
     5461 ttgtctgtta cggagtctcc tgcaacggtc ggatcgccct tgctttctta ttatgatgct
     5521 gtagtggttc attttaagaa ctacgatgac cgccttttac tggacgctgc cgtaggagag
     5581 gcttttcagc gctacgttgc atccggcaag ggcgtagtct ttactcattt cagctgcggc
     5641 gcttttcaga attgggctcc ctttgaaaaa attgcggggc gcatctggaa cccttcgtta
     5701 agagggcatg atccgtacgg agaatttact gtgcggatga ccgggtcttc tcaccctgtt
     5761 gtcgcctcca tgcatgactt tacaaccaga gatgagttgt atacatgtct tgagggcaca
     5821 gttccgataa ctgtcttatg cgatgctgtt tcctgtgttg atcagaaggt ctaccccatg
     5881 gcttttgttg ttgaaaatac aggtgggaga gtgtttcatt gtctcctggg gcacgacccg
     5941 cacgcctacc ggaatgcagc agtcgcagat ttatacagga acgctgtgtg ttgggtctcc
     6001 ggtttgctgg agaatccctg attctggcca ggtgctgaca gaaggctgca gcaaaaagtc
     6061 cttgccaata aagtgccggc ggttgtacaa taaaagaggc accttttttc tcttgcttta
     6121 tgcagtctgt gccgaatcga tgaggatggc tgttcatgat gaccctctta attgcaatcg
     6181 gatcttttgt tggatttctg gtggcgtatc acacctatgg tcgctggctg gcacgaaaaa
     6241 tatttgcctt gaaccccgat gccgctactc cgtcccatac cctcgcagac gggaaagact
     6301 atgttgccac acgtccagcc gttctcttcg ggcatcactt ttcttccatt gccggaaccg
     6361 ggcctattgt cggtcccgcc attgccgtgt tatggggttg gcttcctgct ttgctctggg
     6421 ttctgctcgg ctctatcttt gtgggtgccg tgcatgatct gggctctctt gtcgtgtcca
     6481 tgcgccataa agggcagtct attggcgatg tgtcaggaaa gctgatcagt ttccgatgcc
     6541 gccttctttt cctgagcatt ctttttttcg cactgacgct ggttgtagcg gttttcggat
     6601 tgattatcgc cacgctcttt tccatttatc cgggctcagt tttttcggtc tggtttgcaa
     6661 tgcctctggc tgtttgtgtg ggcgtgctca tctataaaat gaagatgcct cttttctttc
     6721 cgtctgctgt cgccttgagt ctgctttatc ttacggtcta cgcaggtgtc tattacctgc
     6781 cgctgaaact ggacatgccg ctctttgcag gagagggcac atcatttttc caatcgctgc
     6841 attcctctgt aggtctgtgg acggtcctga tcctctgcta ttgttttttg gcgagtgtgc
     6901 tccctgtctg gctgttgctg cagccccggg actatatcag ttcccttcag ttgtatgttg
     6961 ctctgtttgc tcttgtagcc gggcttataa ttgctgcacc gcctattgtt gctccggctc
     7021 ttggcagtgt gggggagggg atgcccccgc tgatgccttt ccttttcata acgattgcct
     7081 gcggtgccgt ttccggtttt cactccgtag tttcgtctgg aacctcatcc aaacaaatcg
     7141 acaacgagcg ggacgcgcag tttatcggat acggctccat gcttgttgaa gcagtgttgg
     7201 ctgtgctggt tattctgagt tgtgttgcag gcgtaggcat gctcattcat acggacagcg
     7261 gcacgcttac cggtcaggca gcattcagcc actattatgg tgtcggctgg gataaaatga
     7321 cgctggcgca cacgatagcg gtttttgtgg aaggagcggg caacctgatt tcttctttgg
     7381 gaatccctcg tgatcttgca tgtggtgtgg tggcagtgat gattgcaggt tttgcgatga
     7441 cgaccatcga cacgacaaca cgccttaatc gctatgtgat tcaggaaatg ggcaacgctg
     7501 ttcaggtaaa aatgctcaac aacaaatttg ttgccacgct ggtggctgtc ggtgtctctg
     7561 cagtactggc atttttgccg gggccgcagg gacccggttc cggggggctt cttctatggc
     7621 cccttttcgg cgcaaccaat cagctcctcg cagggcttgc tttgatggtg gtgatatttt
     7681 acctccgttg gatcgggaaa cctctctttt ttgcgatacc tcctcttctg cttatgctgc
     7741 tcatgcctgc ctgggcaatt gtcaatcagc tttcgggatt ttcagaaaag aaaaattacc
     7801 ttctggtcgc ctttggcacg gcaattctta ttctgcagct gtggatggtg attgaggcaa
     7861 tcattgcctg gcgttccgtg ccggaacgtg ccgctcggga agaagctgtt tcagccgagg
     7921 tgtgtccgaa gcatgaagga tgagccttca gaatcgaagc agttaagggt cttcgccgcg
     7981 cttccgcttc ctgagcagat gcgtgctgtt gtacacagag gcagtgacat ctgcaggaac
     8041 aacctgcagt ctgttgtgcg ttgggttccc ccggaaaatc tgcacctgac cctccgattt
     8101 tacggaacca tcacggaggc acaactggag gttatacaca acacgctatc cgggcgactg
     8161 gcagaatgca ccgctccctt tcttcagctc gctgggataa atgcgtttcc gtccttaaaa
     8221 cgtccttccg tgttttatgt gggcgtaaga ataattcagg gcgatttaca gcaccttgcc
     8281 tctatctgcg aaaaagccgc attgcatgcc gggcttcctg aggaaaagaa gcaattcaaa
     8341 gcgcacataa cgataggacg catcaaaaga aatgctgttg cgccttgtgc tgaagaggtt
     8401 ctttcggaac ccgggggcga ggtgctttcc ggacaggaat tccgggcgga taatgtggta
     8461 ttgtttaaca gcgagctgag ttctcgggga cctctttatt ctgcacttag aaattatcgg
     8521 ctgaaatcat gattgagact gcaatacttc caccaacggc cttcggcgct gctttgtaca
     8581 gtgcgattac cctgtatatg atggcgcttc ttcttcgctg gcttgcccca tttctatctc
     8641 tgaacatcta tgccagaccc tatgtctggc tcaatcctat catcgatcct tacttgaaac
     8701 tgatccgcaa cacactgcct tccatgggtt ttactgattg gtcgcctgtt gcgggaattt
     8761 tggttttgtg ggtgatccgc agttttgtca tgtccatgta acaagggaaa aatgtatgaa
     8821 agccattttt acaggcgtat tccttttggc gttgtgcttt tttgtacttg cttcaggctg
     8881 cattccctct tctccggctc cggttcccgt tgacaaacct gcgccagcaa aaaaagaggc
     8941 tgctgccgcg tcggagacaa agcaacacca gacagcagaa gggatatcca ccctgaatgt
     9001 gcctctccag gtgcagggaa atgcaacggg cgctatttct atgcctttaa taaaacttgt
     9061 cccccagagt gtagaaaata catacaagtt ggaagggact ttcccggtaa aaggaattgc
     9121 tattgatggg gccctggtac tgcagccgga aggaaaatgg tacttgtccg gtaaattcac
     9181 atcggtctat cccagtttta agcccggagc ccccgtttat cagcctatgg gcattttaca
     9241 gccacgtgaa ggacaagggc ttgaggtggt gcagacatcc caggaagggc ttatcctttt
     9301 tgctgtacca atgccttctc ctgatgctga aaaatccgga cagcctgaag aacatccttt
     9361 tgcccttact tttgacgcgt caccgcaaac cgtgttcagc gttatgatca ctccctttta
     9421 agccgtaaga gaaagaagcc atgaccaccg aacacggaga gtcccctgaa tacccgcggc
     9481 tgaaagaact gtgtaaacga cacaatatcc gattcaaaaa acagctcggt caaaaccttc
     9541 tgcttgacga taacattaat accatcatgg tgggtgctgc cggattaact cccgaagaca
     9601 ctgtgataga agtgggctcc gggcttggtg cacttacaaa aaaacttgtc cggcaggcag
     9661 gacggcttct ctgtgttgag atagatccta cctttatgcc ctgtctggaa gaacagtttg
     9721 gcgataaccc caaagtgcag ctgtttcgcg gggatattct taaccatgaa ctggacaaat
     9781 tggtggcaga atttcttccc ggcgccggta ctctgaaaat ggtttccaat ctgccttatt
     9841 atgtgacaac accggttctg tttcattttc ttgagagtcc cctttatttt gagcggctcg
     9901 tggtgatgac gcagctggaa gttggacata ggctgactgc cgccgtgaat tccgaagaat
     9961 acggggtcct ttctgttgcc gccagactct atgcccatac cgacattgtg caccgggttc
    10021 ctgcctcctg ttttctgccc aaaccgaagg tggacagctg tattgttcgc ttccgctgtc
    10081 acaaggaatc accggttccc gggctggaga tagcagaaat tatgaaagtg gttcgcgctg
    10141 ctttccgtca gcgacggaaa accttgcgca acaccctgac tggcggtaat ctcggtttgt
    10201 cgagagatca ggtaatggtt gcactggcag aagcaaatat cgcggacagc cggcgggcac
    10261 agaccctgga ttggactgat ttcggtcgtc tgaccggaat tatccgaaac attaaaaatg
    10321 catcgtaagc acatatcttc tggagaaaag aaaatgaagg ctttctattg tgtcggaacg
    10381 cattgggacc gggagtggta caaaacattt caggagttcc ggatgtggct ggttgagtgc
    10441 gttgataaag caatggattt actggaaaat gaagaaacct ttcgggcttt tcatctggac
    10501 gggcaaacca tcgtcgttga tgattacctc caaatacgcc cggaatcaag agagcgattt
    10561 ctgaagctgg tgaaggagcg ccggattctt atcggaccct ggtataacct ccctgatgag
    10621 tggctgcttt ccggggaaag ccttataaga aatctgatgc gcggttttcg ggattgtgca
    10681 cggctcgggg taaagcctct ggactatgcc tatacacccg atcagttcgg tcatatcgcc
    10741 gcacttccca tgatcatggg cggattcggt tttaaagccg gtatcgtgtg gcgcggtgcc
    10801 caggatgaaa actatccggc acaatttctc tgggaggctc ccgacggaac cgcaatggcg
    10861 tactgtaaac tatccgatgt gggaagttac gggccctttg attttcaggt gagaagaccg
    10921 gttcgtgccg acggatttac agaagaaagt ttcaggaagc attttgaaaa atacttggaa
    10981 agtgaaaaag caagaaattc cgtgccgctg gtgctcatgc tggatgccat tgaccatcag
    11041 gatcctgatc cggaaatggt gcagattttc aaggagctcc gcaggctgta tccccatatt
    11101 gagtttgtct ggtctagcct cgaggacttc ggcgatgagc tgatcaagca taagcagagc
    11161 tatcccgtgc gtaagggaga attgcgggag ccggtacgcg attctgaccg gtacggacat
    11221 tatctcattg tgcataccct ctcttcccgg gtagatatta aaaagcgcaa cgaccagtgc
    11281 tccgcattgc ttgaaaaatg gtgtgagcct gtgctgctta tggcggccat gaacgggatc
    11341 catttccctg agacctatct ggaaaagtcc tgggaatacc tgatgaaaaa ccatccccat
    11401 gattcgattt gcggctgcag tattgaacag gtgcacaggg acatgcacta ccgctatgat
    11461 cagtcggaat taattgctga agggcttgtt cgccgtgctg tggcagcttt gggcgaagcg
    11521 gctgctgatg aagggaaatg gcagcacatt actgtgcata atcccttgcc ttatgcgcgc
    11581 aagggtgtat atgatctcgc cctttatttt ccttcagatt acggacagac aacaggacac
    11641 atttatcatg acgggcttgc tacgggagag cggtacaata agtttcatct ggtgcgttcc
    11701 gacgggcagc gtctcactta tcagcataaa cggattgagc gcaatctgga agtgcctcgg
    11761 ctgaatgagc ggggcagaga aatggctgcg ccgggcgatg tgtaccatgt ggctgttgaa
    11821 ctggaacttc cgccctgcgg cagcacctca tttttcattg aaggtactaa cgatgccacc
    11881 agaacatttg gaagtctgtt gaccgggccg ctttccgctt ccaatggagt gattgaaata
    11941 agtcttgata atcagggagc cctttctctg gaaacaccgg acgggcagca gtacagcggg
    12001 cttctgcagt atgaagatgc gggggattgc ggtgacggct ggacacgggg aattccggtc
    12061 aatgacctcg ttgtacgttc ttatggatcg gcggtgatga catccataga agaaaatggg
    12121 ccgctgcgct gcaccttccg ggtcgagcgg cttttgtctg tgccatcgaa aattgagaaa
    12181 ggaacgggat tccgttcaga agagagggta gacctgcaaa tcatcgatcg gatcactctc
    12241 gaaaaacatt ctccctttgt aaaaatcagg acaattattc gtaattcggc acaggatcac
    12301 cgattgcgtg ttctcttccc gactgattgc gatgcatctg ttacctttgc ggaaacccct
    12361 tttgccgtgg ttgaaagaga gattgaaatc cccgaagagt ctgccctttg gcaggagcgg
    12421 gttaatgaag aaaaagcgtt ctccactttt ttcggtgttc aggacaaaga ccgagggctt
    12481 gccgtgctgt gtcctgcagg gctgcatgag tacgctgcat tggatacacc gcgcactgaa
    12541 ttggcattga cccttttcag aggctttgcc aaaacggtcg gaacacaggg ggaaacagac
    12601 gggcagcttt caggaacaat tgaacttgaa tacgcccttg tacctcttac agaaaaggct
    12661 gacccccgac agctgatcag acatgtctcg gaaatgcagg cgataccgaa acatcacgaa
    12721 tcccgtgaag cgattccgac gaagagtcac ttgtatcagc tgacgcaaaa agcggttatg
    12781 actgctctga agagatcagc agacggcaag ggcggcatcg tgcgcttctg gaaccccact
    12841 gaaaaaaccg tgtcagacgg ctttgcaaca accggagaat tgtctggtgc ctggctgtgc
    12901 aatttgaatg aagagaagga aacagatatt cctgtctcag gcagtacgat tcaaataacg
    12961 atccctgcat tttcattaaa aaccatttgc tttacatgga agcatgaaac gtgatatggt
    13021 taatagcaca ttgcgcagtg tgatggcgga tattgccaat aattctttat caaatgctgt
    13081 aacagtctgt aaaggcaatt gtatcttatt cagatgcatg gagcatcgaa aaacaagaaa
    13141 caacaaagga gaaatcagat gattttactc gaactcttcc gcaaactctt ttccacagct
    13201 ttcgcttttt ttgccgactg gcttagtaat ctcgttatgg gatggattac ccccgaagaa
    13261 gacgatcagg aatagttttc tgtaaaagaa aaatacaacc gctttttaag catttagaaa
    13321 aggaaaaacc atggatttta cagcacttat tggaagtctg gttaacacag cagtgacttt
    13381 cctgcttgac acctttttga acgggctttt tgcctttttg tccgacctct ttttcggagc
    13441 gccgattgcc tgagttctgc tgattagcat taaaaagtta gccgctgttc cagttccaaa
    13501 aggaaatcga gcagcggctt ttttctttat ggcaagaacg caaatcaggg tgtcagcaaa
    13561 atatagtcca gtccgaacat atagtctgtg gaatccggat gttttccaag aatctttaca
    13621 tcaataaaat tgagcccttt ggtaaaatcc gcacgtccaa gggaaacggg ttcagcaaca
    13681 ataacttctc ccgcattgaa cagatccaca gagggcgcaa taacttttcc gttcacggct
    13741 aattcatgaa taccgtaatc aggcgctttt gtgaaaacag cggacacttc atagttgccc
    13801 gcttctgcaa ccgggaagcg caatttaaga tgatcgcccg gttcaccgcc gcgccaccat
    13861 aactggagaa aggaactcca gggccagctg ccgctctgcg catccacggt gccgcgggaa
    13921 acttcaatag cgcgcatctt ttcgccttcc agagcgcctt tcacggcttc cggcactgga
    13981 taaaccggca cggtcagcaa atctgcagac ggttccggga agttatcagt ggcgcctgct
    14041 gctgcgtacc agtatgcggt aatggattga gagataagca catctgccca gtgccaaatc
    14101 tccatatcaa accggaaatc ctttctgaaa ggaatatcat ccataatatg aaaacggctc
    14161 acacaactct gcccgaaatt ggccggaccg tcgcagcgaa tctggttatg gtaggcgtga
    14221 gtgaagacct ctgtgtcaca ccaggcatag ccgaagtagt cttcactgcc tgttccgaag
    14281 aaactcggaa aggtgtctcc gtcgacgtaa atcttttcat caccttctcc ccaccactga
    14341 gttaccggat ttgttatatg aagcagaact ccacagaaac gcccgacacc tgtgcccgac
    14401 atgaagttcc agtcctgcat cggacgggtg ggaatatcct gaacgccctt ccacttggca
    14461 tggaaataga gtgtgtcgtc ggtccaggcg tgcggcgtta taaaaaagtg gagagacagt
    14521 gtcacgggga atcggttaaa attaattaac tcgacttcgg ctttctcttt aaaaggcata
    14581 atccagcggc tgtaaagcgt gttatccgct aatacgccag agggaaggga ctgatagggg
    14641 ttaatgcccg gggctgttcc gaaaaaatca cccagcggtg caacaacaga gggtgcctgc
    14701 ccgtcaaaac tgattttgag gagacagcca cgcagggctt gttccaggtc atcggccgtg
    14761 actttgcatt ggatgccgct gattgctgag ggcccgtcaa aagaacagtc ttcagagtcc
    14821 gcgccttcta ccgttacatc aaaggcctta accgttgcgc ctggagcagt ttcgaagttc
    14881 ccgggatgtt tgaaagcctc ggaaacggcg cgcaattcct tgttgtattt ctctgtatca
    14941 gcaagcgtaa aacttttaac ctctgtgtcg ctcacataag ttctgtaatt gataatgtag
    15001 tagatatcgc ttgcgtcaat agtaactttg cagtgttttt cataaggaat cggcagatag
    15061 ctgttccagc ctttcccgcg ctcgccgcaa ataggactga taaagggctt tgttttcccc
    15121 tccagaagag aagccaaagg catttcaatg accggctcgg atgcgtgatc cagataaaag
    15181 cgaacaacgc cgccttcggt cggatttgca gaccagaacc ggacgatggc gccgggacct
    15241 gccgcatcca tgagcaccca ttccttacgc ccgctctgtt cttccacccg caaatgctgg
    15301 ttgcggtccg tattggcaaa ccaattttct tcggttgcga tggaaggatc ggtggaacgc
    15361 cggtcataac tggaaaactg tccggtcgta taggcttgtt ttccaagagt ggtcaaacgg
    15421 cttaaatcag ccatttcttc aagaagggtg cccaggtcta ccggctgtgc agcggaggca
    15481 ataccggaaa gaagcatgat aaaaaaagcg aagagacaaa aggaattgga atactgaaag
    15541 agagatttct ttggcatggg aactccttgg ttgactgtag aatgttaaaa catgatttct
    15601 tcaaaagccc ggggcggtcc cagaatttca cgcagcacta aagcgcgcct gatttcttcc
    15661 tgagatgaaa atgtgcgaag cacttcttcc gataaaaaag aaacggcacg gggtgctgcg
    15721 gctgggcgca tggtctcctg ccttttgggt gccgctgcag taacgactgc gggcgcggct
    15781 ttaggtttgg actttgcagc agccgggcgg ggtgcacgcc gcggaggttc ctgatgtttc
    15841 cgcgcgggaa gaggggaggc agcggggcgc agcacttctt gcagtgttac aggttgagga
    15901 gacggacgac ctgcagcagg gcgaaacggt gcctcctgcg gcgctaggga gcgtctggca
    15961 gtctgtggat ctcccgcaag aacttgtctg ggctccgaaa aaacaggcgg tctcgtttcc
    16021 gagggcagaa ccgttcgcgg ctcatctgca ggcatacggc gatgctcctc agcaggacgt
    16081 cggggctctg caggcggtac aaaagttctt ccgccgcctt taggtgtcgc caccggaata
    16141 tcccaggatt catccccgta aatcatgcga cgtgtcgcct ccggcagttc atctaccgga
    16201 aagggtctgg gcagggtctc ttctatatcg ggcgtagttg tcttttcttt cgccagcctg
    16261 ctgataacac tgatcacagc aataacaatg aagattaaaa accctaaaag gtcttccatc
    16321 agttgattcc tttcagagat ctttagaaag atttatttaa gctcttctga tgtgccgctt
    16381 ccgggcttgg ctatggactc acgcatattg gtatccgcag aaatgttttt catgcgatag
    16441 tagtccataa tgcccagatt gccgttgcga aaggcttcgg ctatcgccag gggcacctgt
    16501 gcctcagctt ttacaacctg tgcccgcatt tcctgtaccg aggcgaccat ttctgcttcc
    16561 cgcgcccgcg ccatggcccg gcgttcttct gcccgcgcct gagcaatctg cttgtcggct
    16621 tctgcctgtt tgatctgcag gtcggcgccg atgttttcac ctacgtcgac atctgcgata
    16681 tcaatggaaa gaatttcaaa tgctgtcccg gcatccagac cgcgcgccag aactgttttc
    16741 gaaatgctgt ccgggttttc aagcactttt ttatgagact gggaggagcc gatagtggtc
    16801 acaatacctt cgcccacgcg ggcgacaatc gtttcttctg tagcaccgcc tacaagacgg
    16861 cggatgttgg tgcgaacggt tacccgtgct tttgctttta attgaatgcc gtccatggcg
    16921 actgctgcca cggtgggagc gcctttggtc gggtcggggc agtcgattac cttgggacgt
    16981 acagaggtct ggacagcgtc aagaacatcg cgtccggcaa ggtcgatcgc tgttgcctgc
    17041 tggaaagaaa ggtcgatgtt ggccttgttg gcagcaatca gagcctgcac cacccgcaca
    17101 acattaccgc ctgcaagata gtgggtttca agttcattgg tgctgacatt caaaccggct
    17161 tttaccgcca taatccgcga atccacaatg gtagccgcat tgactttccg aagactcatg
    17221 ccgatcagct gcagccagcc gacacttgcc tgagacagcc aggcacgcag ccaaaggcgg
    17281 atataggaaa taaaaacaac caagacaact aaaaagacca caaaaattcc gagcgtgatc
    17341 agacccataa ttgcatccat ggtattgttc cttattcggg ttgttttgag cgttgcgcaa
    17401 ccgcctcgac aagaacacgg ctgccctgta cctgaacgac gacaacgcgg gttccttcgt
    17461 ccagaaacac gccgtcactg actacatcaa tgcgctgggt accaatttta atagttcccg
    17521 ccgggcgcaa agcggagaga acaattcctt ctttgcctat aagactgaga tcggattcag
    17581 cactgacata tcccgcttcc tgtgtctggg catcgttcag cgtaagcaat tgtgatgccc
    17641 gggttttggt caggacgaca aaaccaagga taacacctac gcctgccccc agcagttcac
    17701 cgccgattat cagaatgaaa tagtcgggat aactgcgtac tcccatgccg gcactgatta
    17761 agatcagtaa gccgccgata acgcctaaaa tcccaccagg caggaaaaac tccgaaaaaa
    17821 ttaacagcat tccggagata aaaaataaaa gtatccacca catgatcagg aggggctcct
    17881 atccaggcat tccaaatagg taattagttg agatatttcc acgcggatac ccagcaggac
    17941 attgctttta tcgctgggat ctttgggatc gaactcggac atagattcaa aagtttcaag
    18001 cagtgtgttg tccgtaccgg gcagcaggcg gctctgctca agcagtttat tgaagcggcg
    18061 ggcaaaaggc acgcaggtat aatacccggc tccttgagaa gaaacaactt ccatttcttt
    18121 caataattgc cgcagcatat ggacgataat ttcattgctg ttcatgcagg gattccttgt
    18181 ctgcccgctg gcggacaaca taacggttgc cttccacaag aataatttcc actggagtgc
    18241 ccggatcaag aaaatcacct tccgttacga tatccagcgt tttatttccg aaacgacctt
    18301 tgcctgcagg tctgagcatg gatgctgtga taccaatctg acctatagct ccttgctcct
    18361 ccctagtttg cactgtatag cctgcttcgg caaactgggc atctgccaga agaagacccc
    18421 gtgcaaaaga acttcgcggg aagtatttcc aggtaagaac aacaaaggcg ataaagaggg
    18481 aacctgcaac aagcagcgtg atgccgccat cgcgaagacg gtcaaagtcc cagctgtagg
    18541 tggggatggg aacccgcata agagaaagat agatgccggc aaaaagacag agaatgccgg
    18601 taatgccggt aacaccgaac ccgggaataa aaaagatctc cgcaatgatc agtatgatcc
    18661 ctacaataac caggagcaga tcgagccagc tggcaagccc gaaaatgaga cgggagccga
    18721 agtaaagaga aaggcataca acgccaatca gaccgggcag accgatgcca ggcgttttaa
    18781 tctctatgta aatccctgca acgccgcaaa gaagaagcag acccgcaata agggggcttg
    18841 tcagaaaaga aaagattttt tccggcaagg tcatcttcat agcgacagat gtaaaggaag
    18901 tgtagcccag ttgcttgagt ccctgctcca cactgtatgc gagataagtg acaagagcga
    18961 cttcctgtgc ttcgcgtgct gtcagcgtca gcaggttgcc tgccggactc agcagcctgg
    19021 catcggaggg aagctcagga agaaggtcgg gagcagaaga agcgggcaga tgcgattttt
    19081 ctgcaggagg ctccggcttc ttatcctgag gggcagaccc cgaaaacccg tctgttgaag
    19141 gatgcgttcc ccgaaaaagt ttctccaatt ctttgagcga atcctgagaa aagcccgggg
    19201 cagcagaagc gcttagtgct cccggagaga gtgaagccgt cagcacggaa aaagagcctg
    19261 atcggtattt ttcgattaca cggccttgtt caactttgaa aacagttacg cttccgtcgc
    19321 cggatttcgt gtaataaagt tcaatacggg gatctaccat cgcctcgccg ataagaggat
    19381 tatgtccttt ctcttcgccc agcgctctgt atttggcacg aagaaaagac atggactttt
    19441 cattcatgga ttcagacatt tccacgcccg gcataacagg cgtggaggct ccgatattca
    19501 cgcccggttc catgatgatg gtgtcacagg cataggcaat gagcgccccg gctgaaatgg
    19561 ctccctttcc ggtaacatag gcaatggtgg gaacccgtgc tttaagaatg ctggaggcaa
    19621 tatctatggc ggagtccact ctgccgccaa aagtgtcaat ggtaaaaaca atggcttcgg
    19681 cgtccttgct ttcttctgta actacgcggt cgacaaggac actgacactg tcacggatct
    19741 cgccttcaac agggcagaca agaatgtttt taaccggctt ttcctctgtt gaaggtgtgg
    19801 cataagaaaa aaacagggta gaggcaaaaa gaaaaaaaaa cccgaccgac agcagaagag
    19861 ttctgaaagg catgttggtt gtttgtctga ttcggatcat aaggctgttg gctctacagt
    19921 tagtttttac gtaattctga cagcgtatct tattgtacat gtattgggct gagagtcaaa
    19981 attaataatt agaaaaagac ggtgactccc aataaaaaat cccgccagga cgaatcctgg
    20041 cgggagtatg gaggtataaa acagaaagat ttagcgtttg gagaactgga agcgtttgcg
    20101 tgcgccgggc tgcccgtatt tcttgcgttc cacttcgcgg gcatcacgtg tcagcaggtc
    20161 tttgccgcgc atcgtggtgc gaagttcttc gtctttttct acaagtgccc gggcaatgcc
    20221 cagactgaga gcgcctgcct gccctgcaag tccgccgcct gcacagcgtg cacggacatc
    20281 aatgctgtct ctcagaccgg cagtcactaa aggcagctcc gccatctgta ccagtgtttc
    20341 gcgggcaagg tattcgtcta caggacgacc attgacgata aagagacctg tgccgggttt
    20401 aatccgaacc cgcgcggagg cacgtttgcg tcttcccagt gcaacgattt cattcgtgtt
    20461 tttatctatc acaacagtat ctccgtaggt agtttatttc actgtgtaaa caacagggtt
    20521 ttgcgcttcg tgcggatgtt cggcaccggc atataccctg tattgcgttg ccagttttct
    20581 tccaagccgg ttgccgggaa gcatcccttt tatagcaatt tccatggcgc gggtgggatg
    20641 tttttccagc ataatttcga ggggggtctc tttaatcccg ccaggataac ccgaatgccg
    20701 gtaataaact tttttcctgc ctttggagcc gataacacgc actttggcag cattaataat
    20761 gacgactgaa tcgccacaat caagaaacgg cgtgtattgg ggtttatgtt taccgcgcag
    20821 cagggacgct gccaaagcgg caacacgtcc aatggactgc ccttcagcat caatcaaata
    20881 ccattgtttg ggctcagccc cttgtgtggg gacataggtt ttcacaaaac aatcctccgg
    20941 ttggagtcgt agcgacaata taggttagag ttgatcaatg gaaataacaa taacggggag
    21001 cctaatagta acaaatagaa gcagataaat tcaaatttct tttcgcaatt atttctgcgt
    21061 gagttcctct cctgattcgg gaagtatact ttatagtgaa gagggattgt gacgatcctg
    21121 atagagtgtg attaatgcaa atataataag ggcaactggc gcaagcgaac cggcaagaag
    21181 cccggagcgt atacttgaat attcggcgat gcctccgatc gcccagggga ggacgagtcc
    21241 gccgaaattg cctaacgctg caagaagggc gaacatggaa gcacctccat gaggaaaaat
    21301 atctgctgtg atccctagcc aggtcggcca caggaaactg catgccagcc ccgcaaaaat
    21361 acagaaggac agagcgattc cgggtgcagg gatcagggtt ccggcaagat agcagacgat
    21421 acagaaaaag gcgccggcga agagaactcc gtaggcacca agccgccgga gaatttgtgg
    21481 cgcagcaagg cggcctcctg ccataaataa ggaaaacagc ccgacagcga ttccgcccat
    21541 cgatttgtca aaaccgagcc ctctctcaga aaaagcgggc aaccactgga tgataccgtt
    21601 ttcagtcgct cctataagcc agataatcag cagagcaaaa ataaagcgcg gcttgagaac
    21661 caggtgtaat atactgtccc gggagcgttc tgggtgaaca aggggaggaa atccggtaag
    21721 aaaaatgagt actgtcaacg ccagaggaac cagtgcggcg gctccggcga ggatacgcca
    21781 ggaaatgccc agatgcatgc aggctgatgc gaagaggaca gttgccagcg ccccgatgca
    21841 gtaaaaagcg tgcagatgat tcagtgccga tgcccgccgg tcaacacata cagcggagac
    21901 tatggggctc atcagcatgt cgagtatgcc tgcgcc
//
LOCUS       DAOPWB010000044        21833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2390_length_21833_cov_5.103820, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000044 DAOPWB010000000
VERSION     DAOPWB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 21833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21833
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_21833_cov_5.103820"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1616
                     /gene="rmuC"
                     /locus_tag="PKN92_05855"
     CDS             48..1616
                     /gene="rmuC"
                     /locus_tag="PKN92_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNZ48352.1"
                     /translation="MDPLHLVLIVCLLLSLVAFLVLLLRKTGAALPDDTREELIILRE
                     QLRAATEKEQQMSADLGGYKSQMETVRAEYAAMQARCASAETLIAELKDTVAAFRRDT
                     EQLRTENSMLHKQVAELKTGIEAERKASEEKFALLKEAETKLSDAFKSLSAEALRRNN
                     ASFLDLAKENLEKFQATAKGDLDSRQKAIHELVKPLRESLETVNKRIAEVEQVRTSAY
                     ASLTEQVKSLAMTHEKLRSETNNLVSALRRPATRGRWGELHLQRVVEMAGMQKYCDFT
                     EQMSVNVEGGRLRPDMVIHLPSNKNIVVDSKVSLDGYLNALEAQNEEDKKRYLESHAR
                     QIRNHIDQLSTKEYWKQFEPTPEFVVLFLPAESFFSAALEHDPQLLDDGFRQQVIIAT
                     PTTLIALLKAVAYGWRQEDLAANAKKISDLGNELYERIAVLAEHFEKLHRGLTNATDA
                     YNGAIRSLESRVLVQARKFKELGIASTREIKEGKQVESSLHRQQAPELLPVPAEEETP
                     DAETGSAPGEELRD"
     gene            complement(1652..3265)
                     /locus_tag="PKN92_05860"
     CDS             complement(1652..3265)
                     /locus_tag="PKN92_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48353.1"
                     /translation="MLQFMKRHKLLSGIFIVLLLLFCVIFGYVRFKMRGEHREYAIDF
                     VLPESDGWKQIGDLEVGVAVRDITPSMDDKDTWVDVNGNGKFEKGVDEWNDRNGNGKL
                     DFAWIAGFDMSRPAMGVNDPVWSRALAFRNNGVTIAIVSIDSIGITNERYITIRKMIH
                     EANPDIDHVAFSATHSHEAPDTMGIWSYSLLWRSLFDEGYMRMLQEKTRDAVLEAVAN
                     LKPADTVIAAGYVPEENFIRDSREPKVMDHQLPLAWFKEKGTENTIAVLTSWGMHPEA
                     MGSENLYISSDFVHYFREAMEKGLEGPDAFEGFGGKCVFFTGPVGGLMTQLSIEIKDR
                     YGNAWKDSSIEKAQAQGENLAIIAAQALRSEEARTMTESQVAVSAKTFFLTMGWPFKA
                     ALYLGIVHPGIFNGQAKSEINALRIGEIEIVTTPGEIYPEIVFGGVENPEGADFKIEP
                     VEVPPFFAAMKGALKMNFNLANDEIGYIVPKSQWDQKPPYTYGRDKAPYGEMYTGNPE
                     VAPGIHKVTMDVLNRLHHTLESMPAAENL"
     gene            complement(3409..4533)
                     /locus_tag="PKN92_05865"
     CDS             complement(3409..4533)
                     /locus_tag="PKN92_05865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ48354.1"
                     /translation="MKLRIAMVGACPFPAPQGSQVFMHYTADCLRRQGHEVHLVVYGY
                     GVGEDPPDVPIHRAVRVPFATKTEAGPSLAKPFQDATLALKLHQVVRKHKIQVVCAHN
                     YEALLAALLVRKRPLLYFAHNAMYDELPYFFSHGDVTREIGRFLDRTIPRLADAVIAP
                     HKRLAGYLVARGCEQERVFVVPPPIDASPFEPMPAGSELPPVLYAGNLDSYQNLSLLF
                     KAMGRVRKKYPDARLMIATASSAAHLDAEVIRAASLEELSPFLSQDGVFAAPRVSWSG
                     YPIKMLNAMAAAKAVVACESAAYPLVHEETGLITPDNDVQAYASALIQLLGNASLRTE
                     MGRRAREQILKDHQPETVGARLSEITLSVWESRSKNAGSA"
     gene            complement(4530..6173)
                     /locus_tag="PKN92_05870"
     CDS             complement(4530..6173)
                     /locus_tag="PKN92_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNZ48355.1"
                     /translation="MSSSAELKEGLRAMSDRPRVLLIGLDGFSPDLTAQWIEQGHLPH
                     LAMLQEQGYLGALRGVTPPVTYPAWTTCVTGVNPGRHGITDFTISLPGNYGIRFLNST
                     DRQVPALWNILSSLGKRVCILGVPATYPPEAVNGIMLSGFDSPVADRLDPSYVYPPEL
                     FSQFSQWPFSLFQEHRISSRWYEEALALLEKKIAVQEEIGCRLLQQEAWDLFMIVLNE
                     ADTVSHHFWQLEDTASPRHDPEITAPDSPILRIYRRLDEAVGRFIDAFDDPGLVLVVS
                     DHGFGGAGVQALHINNYLEACGWLSYKKASQGILKKAALRWVPMRLRGTLFRRFQRLV
                     ESLESRARRGGIQWDKTRAWSDELDYCPAIRLNVAGREGKGIIPPEQYASSVSELCAL
                     LEKLPGVARAVPRDTVYKGAFADRAADIFLELDWSTGYRASVIRKRGGAAVEVLQPEA
                     YKGGKEQGCAGVHRNPAFLASSRPLTRKNPAMEDIAPAVLSWLGITYEGMEGKSFLES
                     QVQEETVCSADWIKASEKKYTEEEESLLEKRMKALGYFE"
     gene            complement(6235..6501)
                     /locus_tag="PKN92_05875"
     CDS             complement(6235..6501)
                     /locus_tag="PKN92_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48356.1"
                     /translation="MRFVPPASGTYYIVAYSPIEESGAMPEMKLWLTVGKGSMFRFSD
                     LLEVLPNNIKIQAFFESAVFPGQAFLSSLFMILLTLAGILVAAL"
     gene            complement(6506..6952)
                     /locus_tag="PKN92_05880"
     CDS             complement(6506..6952)
                     /locus_tag="PKN92_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48357.1"
                     /translation="MNRGFIRLAALQLCFFIIAGTAAFAEAPWHYKVVTVNSGGRTMQ
                     SALTIPDANRASIAYHHARLKSPALWLKVEAEAGEAISLRMGVPMLDRYRQARPSVAV
                     LSPHLPKLAEEVPFTVPESMGGLVLSTRLLDSEQYRDTYRCAQLAF"
     gene            complement(7101..8270)
                     /gene="galK"
                     /locus_tag="PKN92_05885"
     CDS             complement(7101..8270)
                     /gene="galK"
                     /locus_tag="PKN92_05885"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNZ48358.1"
                     /translation="MSIHIDDALQAYKRCFDSTPTIAARAPGRVNIIGEHTDYNDGFV
                     LPMAIEHDTLILGVPRQDQVLNMRAETLGRSARLRADVPARSAQEPWTDYVSGVIAEL
                     QKLGYPVSGADLLIVGDVPVGCGLSSSAALEMAVLAFFEKAGGFQMQGPDAARLGQRV
                     ENDFLGLSTGIMDQFISRCGRLGHALFLDCRSYDYTLVPVAFKDALFVIANTACPRGL
                     TASKYNERVSECTAAVEALKHAKDKGNKLRDYTLADLEGVRHAMDPLHFRRARHVITE
                     NARTLDAKAAMESGGLEQLGTLMTASDISLKEDYEVTSPELDVLTAIARSLPGCYGAR
                     MTGAGFGGCTINLVAKNAAESFSAQLLEGYKKETGRTGEVYISAPADGAGAFFLS"
     gene            complement(8267..8725)
                     /locus_tag="PKN92_05890"
     CDS             complement(8267..8725)
                     /locus_tag="PKN92_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNZ48359.1"
                     /translation="MESPFFPGMNKDPETEAEWFSALINLTRFLRSPEGCPWDREHNA
                     GDFARFSVEEGEELTEALASGDNSHAEEEFGDCLFTLLSCVAAAEAEARFSLKGALQR
                     AHMKMMRRHEHVFRKDRARTPEEAMQAWQQIKKQEKAEKNTRTEPKEIGS"
     gene            complement(8798..9706)
                     /locus_tag="PKN92_05895"
     CDS             complement(8798..9706)
                     /locus_tag="PKN92_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNZ48360.1"
                     /translation="MFVPNRFLTIKEQILLLGVMGAVLLGIGTLWLYRTDKEPSAGGT
                     PETAYRRIVPPTPDRTEVRLPVPGSVPGPAAARDASQRTVAGTPPAPRTEGTATAVPE
                     REEVLPAVAANAKDAGMIGVAAMGAVCRPGFYRMRAGDRITDLIEAAGGAAETADLSG
                     LLPTAQLIDGATLTVPFQSYWEGDARSAAVRRPETDHRLNPDAYLRKPLGRTGNASTK
                     ENSTVQHPPAAAQKSGNASAGEKININTASAIELQQLPGIGAVLSSAIVAEREKGSFT
                     SPEDLLRVPGIGEKRLEAVRSLITAP"
     gene            10074..13883
                     /locus_tag="PKN92_05900"
     CDS             10074..13883
                     /locus_tag="PKN92_05900"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HNZ48361.1"
                     /translation="MPTVFRLTALGVLFSTLVLFSGCFDQQPTDKAPSPDFEVSPQEG
                     WAPLIVEFKDASHQGDSKIHAWLWNFGDGITASGPAAKHTYTRPGAYDVTLTVTSSKG
                     NFSKTKVAAVTIKAPGTFGKADPRTGLLTADGAVLKISEPFRAKAQFSIKKGTVPAGL
                     MIPDDVTVLSDVFTIMHNQTSDDFYALDFESKRAPEPANIALPLLSNLDAPVTDDSHL
                     YILAFMEDGRVIPMPGHLARNLFTAAVLRIPQRARYVVVRREDAAIFESKAACPAKAL
                     AAEDKEGWGNVWRIHSSAGVGQMLVSLYNGSTADESSFLKRTWSQTAVTGAHDRLAEA
                     VQAIYDTLKTQDLRPPILISEDLKYDLLFFDTLPSYNVALSRANQVPFYETFFGHLVL
                     NPSLLIDLSRRNARTFHCDPPEQIEFPLSGGEEAQWYGCDFDRAFDPDSAFGEALLYT
                     LYPGYQLPVIATAGDTALNLPVPATDSEEDLPLPVNFVDGLLDSYALYYGQSIQTWPA
                     RAFGVNERPVYSLPLFFPYSGDVAGYSPASHEFLAWLKKSGAVEDPMEVLPWTLEALK
                     PIISIMSAHLARPLNFNEARAALALAFDAALLELDDTEEDLGLPEFYWAYIKDNLFAN
                     SDNAVLRPSDQNRSLWSLNLDKLDASGLLDFTPGGPVENLDFQPENYPVLADIAPLSS
                     RAVVLHFSRLTTDVLLDFSGNKWRKDKRDNGVGIAVYLDGYPGIDLSDAPGDYVHYSL
                     IDKDQDGKTDIVRFSDFTADQEYLLDKVTILISNLNPQLSNSVSFSLQTHADVITPES
                     SILHRYVYSPDPAYGYELTSIYNRPDYSAYLLNMTSGSWRASAELYETEWHHKMAIVE
                     PRNLRENTALLLIAGGSATSEISLQELSAMAEYATVSRTAVVLLKQTPYQPLYFDDQI
                     TGLSEDGIIAYSFNKYMQSYKTGAPDSTWPALLPMTRAAVRAMDTTQTFMAGKPGISR
                     NIDRFVVSGASKRGWTTWLTAAIDPRVCAIVPMVIDVLNMEEQIKHHRAAYSTYPPTS
                     AQYFMQGGYSTALKDYVANSIFTRFDTTEGKSLLSIVDPFYYREVLRMPKLILNSTGD
                     QFFLPDGSQFYFDDLEGLNYLAYLPNSDHSLNNYFGLENSTAKTLLAFHIAQVRNNNS
                     ITSDNVEVPHYTWEFGQIGNKPHIISHASQKPISALQWTATVPDHRDFRLQTRGTIWA
                     SQELEVEEIEAPEEMDGSISWRCVAETVPPAQGWTGFLIQLTFEGPEAKTEFTFTTPV
                     RIISADN"
     gene            13992..15347
                     /locus_tag="PKN92_05905"
     CDS             13992..15347
                     /locus_tag="PKN92_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48362.1"
                     /translation="MALKNENKSLERRDFLKIGAAAGAAALIQNRASASQNTSAFAEA
                     PTAPPIDRVRIGFVGVGGMGSSHLRNLLHLEGVDIVAVCDIVEEKVVKAQDLVEASGQ
                     PRPTGYFKGELDFMRLCTEEDLTLVYTATPWEWHAQVCLAALASGKHAVTEVPLAVTL
                     EDCWRLVESSEKHGKYCIMMENCCYGRTEMTVLNMVRQGVLGEIIHGECGYLHDIRDI
                     FLQIAPGREWFQKHVLTRNGSLYTTHGIGPVAQCMNINRGDQFSYLVSMSSPALGLEA
                     YAREKLPEEDPRRHLKFACGDVNTTLIQTRAGRSISVVFNTCNPRPYTRINLIQGLKG
                     IVEGYPDRVYVEGSSPEHTWEDMEDYWERYEPVLWRELGKDLSGFGHGGMDYLEDYRL
                     IKCLREGKRPDLDVYDGASWSCIADLSEKSVAQKSAPIEIPDFTRGAWKSRPALPVPE
                     A"
     gene            15681..15980
                     /locus_tag="PKN92_05910"
     CDS             15681..15980
                     /locus_tag="PKN92_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48363.1"
                     /translation="MKPSDIGALLFEHENVSEVYVTRFELTDKKETLCAFVVPTASEG
                     LTEKELHEYCAQRMPGNTQEIRIKIIEEIPRNPMGTVERDKLRIYAEQASAKEVA"
     gene            16080..17189
                     /locus_tag="PKN92_05915"
     CDS             16080..17189
                     /locus_tag="PKN92_05915"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ48364.1"
                     /translation="MKIRTLSVLLAGSCLALACSTIHAQDVAEGQWINLFDGESLYGW
                     TPVGDGNWEVSDGVLSLTQGNSGFLATLAQFSDFDLSVTMRVTGMGTAGIAFRAPLSG
                     YAQEQGGAAVNLVAGEDNAAFSTVNIRALGKEVTAQVNDIDVPTAVSNTKGHIAIQFH
                     KYDRFKKGPKLEIKEVKLRPLAMTSLFNGENLDGWNIIPDRKSVFSVNDGALNIKDGN
                     GQIETTGVYRNFVLQLDIYSNGDHLNSGVFFRTPPGVFWVGYEAQIRNQWTRDDRSKP
                     VDFGTGGLYGLKPARKVVSNDREWFTMTVACCDNHYAVWVNGYQTADFTDTRPVSEDA
                     NGKNGYVSTAGTINLQGHDPTTDLSFKNIHIQPLD"
     gene            17362..>18231
                     /locus_tag="PKN92_05920"
     CDS             17362..>18231
                     /locus_tag="PKN92_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNZ48365.1"
                     /translation="MRSVFFSICCFSLFTACFSLSAQQSDNWRMPYEGADVTGDDVLA
                     FWRFDDTDDSVPDSSGKGHHGKLDGAVLSSEGKFGGSLQSFPGWPADDKRHGAVIPAS
                     PSLSPSGAFTLELWLCPDEFPEGYGECFLIDKKYVAHTDYQWILDQPDSGGMQRMRLT
                     LGFGETSETWWSPESISLKPGVWNHLAVTYDGAGKTTFHLNGRSLGSLTKPGRGSMQA
                     GTNPLVLGDRIGSYHHGFPGRIDEIRLTRGVREFQPCLITPAYERSVYRRFEAPPTLS
                     YRITNIGKSDLPVV"
     assembly_gap    18232..18241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18242..20614
                     /locus_tag="PKN92_05925"
     CDS             <18242..20614
                     /locus_tag="PKN92_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48366.1"
                     /translation="DRDALLSREISLAAGETALLDIPFDTKLRPDAYAMRIAYSITEG
                     AEVFENELYFPVKITPRQPSARMPVVMWGIGGVKEVEKALPTLKDIGFTHCLGLRCDF
                     ERIREADSPVPAVTEKEYADSCAMLDLALENDLGIIIGISPGHWLSEREETERLDKDG
                     KPYSRRNVCCNFPEAPAFAENVGRSVARTFGSFPAFTAAMIDTEVRDGTQLCFHDHDR
                     DLYRQATGLEFPAIAVNKSGVSYSDLPDFPQDRVIENDNELLRFYRWFWKEGDGWNAF
                     HSAVCKGLKRDDRPDFWTYFDPAVRVPPLWGSGGSVDYLSHWTYSYPDPLRMGLAADE
                     LFAMAAGGPAGQQVMKMTQIIWYRSQTAPLTEGGPQGPRSAWEDYDPDAAYITIAPAH
                     LREAFWHKIARPVKGIMYHGWQSLVETEGKSVYRYTHPETRFALQDLIETVVKPLGPT
                     LMQLPEAPSDVAFLESFTSTIFAGRGTYGWGGSRAADVYHMLLYAQMQPRIIYEETLL
                     TEGLDNFRVLVLADCDVLPREVVEQIQAYQKKGGIIVGDDRLCPAIRPDIVVQVETRS
                     GKADEDKGRILAGAARLAAQLAPVYRPAIISSDPEVLPYKRSFGPADYVFAINDKREF
                     GSYVGQHGLVMENGVPAQAAFTLSRNEGFVYDLVRHRQVDASSEDGHLRIPITLEPGG
                     GRMYLVCENTIDQVQITCADHASRGEEISFSISVCDSTGKAADALIPLELRITDPDGR
                     PAEFSGFYGAPSGSLNLTAAIAANDTPGMWTLEVRELASNRQGFHFFRVD"
     gene            20663..21355
                     /gene="yidC"
                     /locus_tag="PKN92_05930"
     CDS             20663..21355
                     /gene="yidC"
                     /locus_tag="PKN92_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNZ48367.1"
                     /translation="MQFLSESIVQIIVFLAGTAGSYGIAILLFAVALKALLLPVTLYG
                     MKWQERFQAAFKSAKEEIAGLSITDAAQRDDEILNIYQAHGINFTNQCKALLPLLIQL
                     PLLVAFYRTMIRWEGLAEHSFLWIRNLQEVDALIPLGISLPWLGSSVNLLPFLLFLVN
                     ASEVILFRDETTGIKSFSLPLIFFFLFYPFPSSCMLFWIMLNVVHIPERWYYVKFRSP
                     VSDASGTSSSSA"
     gene            complement(21295..>21833)
                     /locus_tag="PKN92_05935"
     CDS             complement(21295..>21833)
                     /locus_tag="PKN92_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48368.1"
                     /translation="EVPLPDFPEMTAKITVTNKSRDPFQFTRLLLRSDLEDEDGLIHI
                     AGRSANRALVEIGPLEEPRILSFLDSWYPGWSVKIDGDPAPLLKVNERFKGVVVPPGT
                     HKVEFVFNPVLTWRSLAFSLFLFILVLTGMSFTGMYTLRTRYIKKGKASGLDASSTTQ
                     AEEDEVPEASETGERNLT"
BASE COUNT         5288 a         5933 c         5529 g         5073 t
ORIGIN      
        1 cggttctagg aagggctctt tctcattcaa cctaagggga actgcatatg gatccgcttc
       61 atctggtact gattgtatgc ctcctgctca gccttgtcgc ctttttagtc ctgttgctca
      121 gaaaaaccgg tgcggccctc cccgacgaca ctcgggagga actgattatt cttcgcgagc
      181 agctccgggc agccactgaa aaagaacaac agatgagcgc tgatctcggt ggatataaaa
      241 gccagatgga aacggtacgc gccgaatatg cagccatgca agcgcgctgt gcgtcggcag
      301 aaacgctcat tgcggaatta aaagacaccg tggctgcgtt ccgcagggac accgagcagc
      361 ttcgtactga aaacagcatg ctccacaagc aggttgccga gctaaaaaca ggcatagaag
      421 cagagcgcaa agcaagcgaa gaaaagtttg ctttgttgaa ggaagcggaa acgaaactct
      481 ctgatgcttt caagtctttg tcggcagaag cccttcgaag aaacaacgct tcctttttgg
      541 atcttgccaa agaaaatctg gaaaaattcc aggccacagc caagggcgat ctggattccc
      601 gacagaaagc catacatgag ctggtgaaac cgctgcgcga gtctctggaa acggtaaata
      661 agcgtattgc ggaagtggaa caggtacgaa caagtgccta cgcctctctg accgagcaag
      721 tgaaatcgct tgccatgacc catgaaaaac tacgctctga aaccaataat ctggtgtcgg
      781 cattgcgcag accggcaaca cggggacgct ggggcgaatt gcacctgcag cgtgtggtcg
      841 aaatggcggg catgcagaaa tactgtgatt ttacagagca gatgtctgtg aatgtggaag
      901 gagggcggct gcgccccgac atggtgattc atctgccgag caacaaaaat atcgtggtag
      961 attcaaaagt gtcgctcgac ggctatctga acgccctgga agcacagaat gaggaagata
     1021 agaaaagata tttagaatcc catgcccggc agatacgcaa ccatatcgac cagctcagca
     1081 ccaaggaata ctggaaacaa tttgagccca cgccggaatt tgtcgttctc ttcctgcctg
     1141 ccgagtcttt tttcagtgcg gcactggaac atgatccgca gctgctggac gacggcttcc
     1201 gccagcaggt tatcatcgca acgcccacta ccctcattgc actgctgaag gcggttgcct
     1261 acggctggcg ccaggaagat ctcgccgcca acgcaaaaaa gatcagcgat ctgggcaatg
     1321 aactctacga acggattgcc gtgctggcgg agcattttga aaaactgcat agaggactga
     1381 caaacgccac cgatgcttat aacggcgcga tcagaagtct ggaaagccgt gtgctggtac
     1441 aagcacgtaa atttaaagag cttggtatcg cttccacccg tgagatcaaa gaaggaaagc
     1501 aggtagagtc cagcctgcat cgacagcagg cgccagagct gcttcctgtc ccggctgaag
     1561 aagaaactcc ggatgccgaa acgggatcgg cacccggaga agaacttcgg gattaaatat
     1621 cctttttaca tccaggaacc ggcagacggg gttagaggtt ttcagcggca ggcatagact
     1681 ccagtgtatg gtggagcctg ttcagcacgt ccatggtcac tttatgaatg cccggtgcca
     1741 cttccggatt gcctgtatac atctcgccat agggtgcctt gtcccgtccg tatgtataag
     1801 gcggtttctg atcccactgc gatttcggta cgatatagcc gatctcatcg ttggcaagat
     1861 tgaaattcat cttgagcgct cctttcatgg cagcaaagaa aggcggcact tcaaccggct
     1921 caatcttaaa gtccgcccct tccggatttt caacgccgcc gaatacaatt tcagggtaaa
     1981 tttcaccggg cgtggtgaca atttctattt cgccgatacg gagcgcattg atctcgcttt
     2041 tcgcctggcc attaaaaatc ccgggatgaa caataccgag atacaaagcg gctttaaagg
     2101 gccagcccat ggttaaaaag aaggtttttg cacttactgc cacctgactt tctgtcatgg
     2161 tgcgtgcttc ttcgctgcgc agtgcctgtg ctgcaataat tgccagattc tcaccctgcg
     2221 cctgggcttt ctctatgctg ctgtccttcc aggcgttgcc gtaacggtct ttaatctcaa
     2281 tgctcagctg agtcatcaga ccgccaaccg gtcctgtaaa aaacacacac ttcccgccaa
     2341 agccctcgaa ggcatccgga ccttccagtc ctttttccat cgcttcgcga aaataatgaa
     2401 caaagtcgga actgatatac agattttcgc tgcccatcgc ttcaggatgc atcccccagg
     2461 aagtgagcac tgcaatggta ttttctgtgc ctttttcttt aaaccacgcc agcggcagct
     2521 gatgatccat taccttcggc tcgcggctgt cccggataaa attttcttcg ggaacataac
     2581 cagcggcaat gaccgtatcg gcaggcttca gatttgcaac cgcttcaagt acagcatccc
     2641 gggtcttttc ctgaagcatc cgcatatacc cttcatcaaa gagcgaacgc cagagcaggc
     2701 tgtaactcca gatccccatg gtatcaggtg cttcatggga atgggtcgcc gaaaatgcga
     2761 catgatcaat atcaggattg gcctcatgaa tcattttgcg tatggtgata taacgctcat
     2821 tggtaatacc gatgctgtca atactgacga tcgcaatggt aacaccgttg tttcgaaaag
     2881 ccagcgcccg ggaccagacc ggatcattca cgcccatggc aggacgggac atatcaaaac
     2941 cggcgatcca ggcaaaatca agtttcccgt tgccgtttcg atcattccac tcatcaacac
     3001 ctttttcaaa tttaccgttg ccgttcacat caacccaggt gtccttgtca tccattgacg
     3061 gggtaatgtc acggacagcg acccccactt caagatcccc gatctgtttc cagccgtcac
     3121 tttcgggcaa cacaaaatca atggcatatt cccgatgctc gccccgcatt ttgaagcgga
     3181 catatccgaa aataacacaa aaaagaagga gcagtacaat aaaaataccg gacagtagtt
     3241 tatgtcgttt cataaattgc agcattagta atctcccggt gttgcattaa cggcgacaag
     3301 tcaggctctg aaatacactc agaaccgcgg tgtgcgatgt ggaatggaaa aatagcggat
     3361 aagataacaa accacagtgc tattatactc aggggaaagt gcgacaaatc aagcacttcc
     3421 ggcattttta ctccggcttt cccagacaga aagcgttatc tcggagagcc gtgccccgac
     3481 agtttccggc tgatgatctt tgagtatctg ttcgcgggca cgacgcccca tttcggttcg
     3541 gagcgaagca ttgcccaaaa gctgtatcaa agcggacgca taagcctgaa catcattatc
     3601 aggggtaatt aatcctgtct cttcatgaac cagaggatag gcggcacttt cacaggcaac
     3661 gaccgcttta gcggcagcca tggcattgag cattttaatg ggatagcccg accaggacac
     3721 acggggtgcg gcgaagactc cgtcctgcga taaaaaagga ctgagttcct caagggaagc
     3781 agcgcgtatc acttccgcat caagatgagc cgcagaagaa gcggttgcga tcatcagccg
     3841 ggcatcggga tattttttgc gcacccggcc catcgcttta aaaagcagag aaaggttctg
     3901 gtaactgtcc agatttccgg cgtagagcac aggaggcagt tcagaccctg ccggcatcgg
     3961 ctcaaaaggc gatgcatcaa tgggaggcgg cacaacaaag acccgctcct gctcgcaccc
     4021 ccgcgccaca agatagcccg ccaaccgctt atggggcgca atgacagcat cggcaagacg
     4081 cgggatagta cgatccagaa aacgccctat ctcacgggtg acatcgccat gagaaaaaaa
     4141 atagggcaat tcatcgtaca tggcattatg cgcaaagtag aggaggggcc gtttgcgtac
     4201 gagcaatgcg gcaagcagcg cttcatagtt atgtgcgcag acaacctgta ttttgtgctt
     4261 gcggactacc tgatgcagtt taagagccag tgtggcgtcc tgaaatggct ttgcgagaga
     4321 aggacctgcc tcggttttgg tggcaaaggg gactcgcacc gcccggtgaa tgggcacatc
     4381 aggaggatct tcgcccaccc catagccgta tacaaccaga tgcacctcat gcccctgccg
     4441 gcgcaggcaa tctgcagtgt agtgcataaa cacctgactg ccctgcggtg caggaaaagg
     4501 gcatgccccg accatggcga tacgcagctt cattcgaaat atcccagcgc tttcatccgt
     4561 ttttccaaaa gagattcttc ctcttcagtg tacttttttt cagacgcctt gatccagtct
     4621 gcagaacaca ctgtctcttc ctgtacctgg ctttccagaa aagatttccc ttccataccc
     4681 tcgtacgtaa tgccaagcca ggaaagaaca gcaggcgcaa tatcctccat ggcgggattc
     4741 ttgcgggtga gcgggcggct tgatgcaaga aaagccggat tccggtgcac tccggcacat
     4801 ccctgctctt ttccgccttt atacgcctca ggctgcagta cttctacagc cgcgccgccc
     4861 cgtttgcgga tgacagaagc gcggtatccg gtcgaccagt ccagttccag aaaaatatcc
     4921 gctgcccggt cggcaaatgc acctttataa acagtatccc ggggcacagc gcgtgccacg
     4981 cccgggagct tttccagaag ggcacacaat tcagacacgg aggaagcata ctgttccggc
     5041 ggaataatgc ccttcccttc cctgcccgcc acattcagcc ggattgcggg gcagtaatcg
     5101 agctcatcag accacgcccg ggttttatcc cattgaatgc cgcctctgcg tgcacgactt
     5161 tccagacttt caaccagccg ttgaaaccgc cgaaaaagag taccccgcag acgcatggga
     5221 acccagcgca gcgctgcctt tttcaatatg ccctgcgacg cttttttata agacagccag
     5281 ccgcaagcct ccagataatt attaatgtgg agagcttgta cacctgctcc gccaaatcca
     5341 tggtcagata caacaagcac aagtcccgga tcatcgaagg catcaatgaa acgaccgact
     5401 gcttcatcca gacgtctgta gattctcaga atgggagaat caggtgccgt gatctccgga
     5461 tcatggcggg gtgatgcagt atcttccagt tgccagaaat ggtgcgacac cgtatccgcc
     5521 tcattgagaa caatcataaa cagatcccag gcttcctgct gcaaaagacg gcacccgatt
     5581 tcttcctgga cagcaatttt cttttccaga agagccagcg cctcttcata ccatcgggaa
     5641 gaaatgcggt gctcctgaaa aagagaaaag ggccattgtg aaaactgtga aaataattca
     5701 ggcgggtaaa catacgaagg atccaggcga tccgccaccg gagaatcaaa gccggacagc
     5761 ataatgccgt ttaccgcttc aggaggataa gttgcgggca cgccgagaat acaaacacgc
     5821 tttcccagtg aagacaggat attccagagc gcgggcacct gcctgtcggt actgtttaaa
     5881 aaccgtatgc cgtaattgcc ggggagggaa attgtaaaat cagttatgcc gtggcgcccc
     5941 ggattcacgc cggtaacaca ggtagtccag gcagggtacg ttacgggagg cgttacaccg
     6001 cgaagggcgc ccagatatcc ttgttcctga agcatcgcca gatgcggaag atgcccctgt
     6061 tcaatccatt gagcggtgag atccggggag aacccatcca gcccaatgag taaaacgcgg
     6121 ggacggtcgc tcatggcgcg caacccttct ttcagttcag cggaagaaga caccccgcag
     6181 caaccggttt gcgggtaaaa ttgacctgta ccaacactgt ttggcggcgg tgtgctaaag
     6241 cgcagccacc agaataccgg caagagtgag gagaatcata aaaaggctgc tcaaaaacgc
     6301 ctgccctgga aaaacggcac tttcgaaaaa agcctgtatt tttatattgt tgggaagcac
     6361 ttcaagcagg tcgctgaaac ggaacatgct ccccttgccc accgtcagcc agagcttcat
     6421 ttcaggcatt gccccgctct cttctatggg cgaataagcg acaatataat aggtgcccga
     6481 cgctggcgga acaaaacgca tttcttcaaa acgccagctg agcacacctg taggtgtcgc
     6541 gatattgttc actgtcgagc aggcgggtag acaaaacaag tccgcccatg gattcgggca
     6601 ccgtgaaagg cacctcttcc gccagtttcg gaagatgcgg ggacagaact gcaacagaag
     6661 ggcgcgcctg acgatagcga tcgagcatag gcacacccat cctgagggag attgcctctc
     6721 cggcttcagc ttcgaccttc agccacaagg cggggctttt caggcgggca tgatgatagg
     6781 caatagatgc cctgttcgca tcgggtatgg tcagtgctga ctgcatggtt ctgccgccgc
     6841 tattcaccgt cacgacctta taatgccagg gcgcttcggc aaaggctgcc gttcccgcta
     6901 ttataaaaaa gcagagctgc agggcagcca ggcggataaa gcctctgttc ataatcgttt
     6961 tcctgtgctt attgatggca aagccggaaa aaaccggcaa atacacaaat gttctcctgt
     7021 tgaaagcata ccccatccag aagccctatg acaatatccc ttgcctgttc cggcttctca
     7081 gactggcata tcgttcccgg tcaggagagg aaaaaagcgc ctgcaccgtc ggcgggagcg
     7141 gatatatata cttctcctgt acggcccgtt tcttttttgt agccctccag cagctgcgca
     7201 gaaaaggact ccgccgcatt ttttgcaacc agattaatgg tgcagccacc aaaaccggca
     7261 cctgtcatcc gggctccata gcaccccgga aggctccgtg cgatggcagt caggacatca
     7321 agttccgggc tggtgacttc gtagtcctcc ttcaaactga tatccgacgc tgtcatcaag
     7381 gtgcccagct gttccaaacc acccgattcc atggcggcct ttgcgtccag agtgcgtgca
     7441 ttttctgtaa tgacatgacg ggcgcgtctg aagtgcagcg gatccatagc atggcgcacg
     7501 ccttccaaat ccgccagcgt gtagtctctc agcttatttc ccttatcttt tgcgtgcttc
     7561 agtgcttcca cggcagcggt acattctgaa acccgctcgt tgtatttgga ggcggtcagc
     7621 ccgcgtgggc acgccgtgtt tgctattaca aacaaagcgt ctttgaacgc aacaggcacc
     7681 agcgtgtaat cataactccg gcaatccagg aaaagagcat gccccaatcg tccgcatcgg
     7741 gaaataaact gatccatgat gcccgtgctc aaccctaaga aatcattctc aacacgctga
     7801 ccgagccgcg cggcatccgg accctgcatc tgaaaaccgc ccgccttttc gaaaaaagca
     7861 agcactgcca tttccagcgc ggcggaactg ctcagcccgc atcccaccgg cacatcaccc
     7921 acgatcagca aatccgcacc gctgacaggg tagcccaact tttgcaattc agcaatcact
     7981 ccggaaacat aatccgtcca cggctcctgc gcactccgtg ccggtacatc ggcgcgcagc
     8041 ctggcagaac ggcccagcgt ctccgcacgc atgttcagca cttgatcctg gcgcggcaca
     8101 ccgaggataa gcgtatcatg ttcaattgcc atgggaagca cgaatccgtc gttgtaatcc
     8161 gtatgttcac caataatatt taccctgccg ggtgcccggg cggcaatggt cggagtgcta
     8221 tcaaagcagc gtttataagc ctgcaacgca tcgtcaatgt gaatgctcat gatccgatct
     8281 ccttcggttc tgttcgtgta ttcttttcag ctttttcctg ctttttgatt tgctgccaag
     8341 cctgcatggc ttcttcgggt gtccgtgccc ggtctttgcg gaaaacatgc tcgtgccttc
     8401 gcatcatttt catgtgagcg cgctgcaacg cacctttaag agaaaaccgt gcttccgcct
     8461 cggctgcagc cacgcaggaa agcagtgtga agaggcagtc tccgaactcc tcctctgcat
     8521 ggctgttatc tcctgatgcc agcgcctcag taagttcttc gccctcttcc acagaaaatc
     8581 gggcgaaatc tccggcatta tgctcacgat cccaggggca gccctccggc gaacgcagga
     8641 agcgcgtcaa attgataagt gcggaaaacc actccgcttc tgtttccgga tctttgttca
     8701 tgcccgggaa aaaaggggac tccataactg ctcctttctc agtttgcagg cagattatgc
     8761 accatacgct cctctttttt acagccggaa ggtgcggtca gggggctgta ataagagatc
     8821 gcacagcctc cagcctcttt tcccctatac ccggtacccg cagcagatct tcaggagagg
     8881 taaatgaccc cttttcccgt tctgcaacaa tggctgaaga aaggacggct ccaataccgg
     8941 gcagctgctg cagctcaatg gcacttgccg tattgatgtt gatcttttca cctgccgaag
     9001 catttcccga tttttgtgct gcggcgggag gatgctgtac agtgctgttt tcttttgttg
     9061 aagcattacc tgttctcccg aggggcttcc gaaggtaagc atccgggttt aatctatgat
     9121 cggtttccgg acggcgaacc gccgcactgc gggcatcccc ttcccagtag ctttggaaag
     9181 gcactgttag cgtggcgccg tcaataagct gtgctgtcgg gagcagcccg gacaagtcgg
     9241 cagtttccgc agcaccaccc gccgcttcga ttaagtccgt tatacgatct cccgcccgca
     9301 ttctgtaaaa cccgggtcgg cagactgcgc ccatggctgc cacacctatc attccggcat
     9361 cctttgcgtt ggctgcaacc gccggaagca cttcttctct ttcaggtacc gccgtggctg
     9421 tcccttctgt acgtggggca ggaggcgtcc ctgcaaccgt ccgctgcgaa gcatccctcg
     9481 cagcagcagg gccgggaact gatccgggca ctggaagccg cacctccgtc ctgtccggcg
     9541 tgggcgggac aatacgacgg tatgccgttt ccggtgttcc tccggcggaa ggttctttgt
     9601 ctgtccgata aagccacagg gttccgatac cgagaagcac agcccccatt acgccgagca
     9661 gcagtatctg ttctttgatt gtaagaaatc tgtttgggac aaacatgcga ctcctcccgg
     9721 gttggcatgc attcactgta ccgttcgaaa aaggttcttc attatgcatg acagtaaaga
     9781 aaaaaggcaa cagccgactg tgtattttca gccacgtgag cgcataagaa aagtacagcc
     9841 gaagcaggcg gcaacggcgt tttaaaaaga agcggcatac gaaaaaaaga aaattaaaaa
     9901 tcttgccaga cccactgaag gtactatata ttagagtagc gcctggaagg caaagtaaat
     9961 acttgacaaa agatgtcaaa ctaagtatta ttttggtatt aaaatgagga ttgtttatcc
    10021 gaaaatcagt gcgggtctgc ttgtgcaact attgctcagg aaaggttttt gtcatgccga
    10081 cagtatttag gctcacggcg ttgggtgtac ttttcagcac attggtgctt ttcagcggct
    10141 gttttgacca gcaaccgact gataaggcgc cttctcccga ctttgaagta tcccctcagg
    10201 aaggctgggc tcctctgatt gtggagttca aggatgcttc ccatcagggc gactcaaaaa
    10261 tccatgcctg gctatggaat ttcggcgacg gaatcacggc atccgggcct gcggcaaaac
    10321 atacatatac ccgtcccggc gcatatgatg tgaccctgac tgtgacaagc agcaaaggta
    10381 atttttccaa aactaaagtt gcggcggtga cgatcaaagc acccggcact tttggaaaag
    10441 cagacccccg caccggcttg ctgacagctg acggtgctgt tctaaaaatc agcgagcctt
    10501 tccgtgcaaa ggcccagttc agcattaaaa aaggtactgt acccgccgga ttaatgattc
    10561 cggacgatgt aactgtcctg tctgacgtat tcacaatcat gcacaaccag acctctgatg
    10621 atttctatgc gttggatttt gaatcgaaga gggcaccgga gcccgccaat attgcactgc
    10681 ctctgcttag caatctcgat gcccctgtca ccgatgactc ccatttgtac atactggcgt
    10741 tcatggaaga cggcagagtc attccgatgc cgggacacct tgccagaaat ctttttaccg
    10801 cagcagtact gcgcatcccg cagagagcgc gctatgtggt tgtccggcgt gaagatgctg
    10861 cgatctttga atccaaagcg gcatgccccg ccaaggcgct cgccgcagag gacaaagaag
    10921 gttggggcaa tgtctggcgc atccacagca gcgccggtgt agggcagatg ctcgtctcgc
    10981 tgtacaatgg ctccactgcc gatgaaagtt cttttcttaa gcgcacctgg tcacaaacag
    11041 ccgtaaccgg cgcccacgac agattagccg aggcggtgca ggcaatctat gacacgctga
    11101 aaactcagga tctgcgcccg cctatcctga tctcggaaga tctgaagtat gacctgctct
    11161 tttttgatac cctgccctct tataatgtag ctttgagccg cgccaaccag gtgccttttt
    11221 acgaaacttt cttcggccat ctggtattaa atccgtctct tctgattgat ctttcccggc
    11281 gtaatgcgcg cacgttccac tgcgacccgc cggagcaaat cgaattccct ctgtccgggg
    11341 gagaagaagc acagtggtat ggctgcgatt tcgaccgggc ttttgatcct gactctgcct
    11401 ttggcgaagc cctcctctac acgctttatc cgggctatca gctgcccgtt attgcaacag
    11461 ccggcgacac cgctctcaat cttccggttc cggcaaccga ttcagaggaa gatctcccgc
    11521 tgccggtgaa ctttgttgac gggcttctgg acagctatgc cctgtactat ggacagtcca
    11581 tacagacctg gcccgcccgc gctttcggtg taaatgaacg acctgtttat tcgcttccgc
    11641 ttttcttccc ctacagcggc gatgtggcag gctacagccc tgccagccac gaattccttg
    11701 cctggctgaa aaagagcggt gccgtggaag atcctatgga agtgcttccg tggacactgg
    11761 aggctctaaa acccattatc agcataatgt ccgcacatct cgcccgaccg cttaatttta
    11821 acgaggcccg tgctgcgctg gctctcgctt ttgacgcggc gttgcttgaa ctggatgata
    11881 ccgaagagga tcttggactg cccgaatttt actgggcata tataaaagac aacctttttg
    11941 cgaacagcga caatgcagtt ttgcggccca gcgaccagaa caggtctttg tggtctttga
    12001 accttgacaa actggatgcg tccgggctgc ttgattttac tccgggcggt cccgtagaaa
    12061 acctggattt ccagccggaa aactatccgg tactcgccga tatagcgccc ttgagcagcc
    12121 gtgccgtagt gctgcacttc agcagattga cgacggatgt tctgctggat ttcagcggca
    12181 ataaatggag aaaagacaaa cgggacaacg gtgtcggcat tgctgtctat ctggacggct
    12241 atcccggcat tgacctttcg gatgctcccg gagactatgt ccactatagt cttattgaca
    12301 aggatcaaga cggaaaaacg gacatcgttc gtttctccga ctttaccgcc gaccaggaat
    12361 atctcctgga caaagtgacc atcctgatct cgaatctgaa tccgcaactg tccaactcgg
    12421 tatcgttctc actgcaaacc catgcggatg tgatcacgcc ggaaagttct attcttcacc
    12481 ggtatgtcta tagcccggac cccgcttacg gctatgaact gaccagtata tacaaccggc
    12541 cagactactc cgcctacctg ctgaatatga cttccggcag ctggcgtgcc tccgctgagt
    12601 tgtatgagac tgaatggcat cacaaaatgg ccattgtgga acccagaaat ctccgggaga
    12661 atacagcgct cttactcatt gcaggcggat cagcaacatc cgagatctcc ctgcaggaac
    12721 tcagcgccat ggcggagtat gcaacagtca gccggacagc ggttgtactc ctcaagcaga
    12781 caccctatca gcctctttat tttgatgatc agatcacggg gctgagtgaa gacggcatta
    12841 tcgcctacag cttcaataaa tacatgcaga gctacaaaac gggcgctccg gatagcacct
    12901 ggccggctct gctgcccatg acccgtgccg ctgtccgtgc catggatacc acccagacct
    12961 tcatggcagg caagcccggc atttcccgga atatcgaccg atttgttgtg agcggcgcat
    13021 ccaagcgcgg ctggaccacc tggctgacag cggcgataga tccccgtgtc tgtgccattg
    13081 ttcccatggt cattgatgta ttgaatatgg aggagcagat caagcaccac cgcgccgcct
    13141 actccactta tccgcccacg agtgcgcaat attttatgca gggcggttac tccacagcgc
    13201 tcaaggacta tgtggcaaat tctattttca cccgctttga taccacagaa ggtaaatctc
    13261 tgctgagcat tgtggatcct ttctactaca gggaagtgct gcgcatgccc aaactgatcc
    13321 tgaattccac aggcgaccag ttcttcctgc ctgatggctc ccagttctat tttgacgatt
    13381 tggaagggct gaattatctt gcttatctgc cgaactccga ccactctctc aataattatt
    13441 tcgggctgga gaattccacg gcaaaaacgc tgcttgcctt ccatatcgca caggtacgca
    13501 acaacaattc catcacatcg gacaatgtgg aagtgcctca ctacacctgg gaattcgggc
    13561 aaatcggcaa caaaccacat attatttccc atgcttcgca gaaacccata agtgccctgc
    13621 agtggactgc cactgttccg gatcaccgtg acttccgcct gcagaccaga ggaacaatct
    13681 gggcctctca ggaactggag gtggaggaaa tagaagctcc ggaagaaatg gacggcagca
    13741 ttagctggcg atgcgtggct gaaactgtcc cgcctgcgca aggctggacc ggtttcctca
    13801 ttcagttaac tttcgagggt cccgaagcaa agaccgaatt tacatttacc actccggtgc
    13861 gcattatttc tgcagacaac tgaaaggtct gtatgtcttt cttttcagac tgcatttaga
    13921 agaatttttc ggcggcaggc ttcaacggct ctgccgccat tttcattgac acgctaaaac
    13981 gaaagaatac tatggctctg aaaaatgaaa acaagtcttt ggaacggcgg gactttttaa
    14041 aaatcggtgc tgcagcgggt gcggcggctc tgatacagaa tcgcgcttca gcctcgcaga
    14101 acacgtctgc ctttgcagaa gcaccgaccg caccgcccat agacagagtg cgtattggtt
    14161 ttgtgggcgt agggggcatg ggatcaagcc acctccgcaa cctgctgcat ctggagggcg
    14221 tggacattgt cgctgtatgc gatattgtcg aagaaaaagt tgtgaaagct caggatctcg
    14281 ttgaagcttc gggccagccc cgaccgacgg gctatttcaa gggcgaactt gatttcatgc
    14341 gcctctgtac cgaagaagat ttaacgcttg tctacacagc cacaccctgg gaatggcatg
    14401 cgcaagtctg cctcgccgcc ctggcttccg gcaaacatgc ggtcactgaa gtgcctcttg
    14461 ccgtaaccct tgaagactgc tggaggctgg tggaatcttc tgaaaaacac ggcaaatact
    14521 gcattatgat ggaaaactgc tgctacggcc gcacggaaat gaccgtgctg aatatggtac
    14581 gccagggcgt tttaggcgaa attattcacg gagaatgcgg ttatctccat gatatccgtg
    14641 atattttcct gcagattgca cccgggcggg agtggtttca aaagcatgtc ctgacgcgca
    14701 acggcagtct gtacaccacg cacggtatcg gtcctgttgc ccaatgcatg aatatcaacc
    14761 ggggcgacca gttttcttat cttgtatcca tgagtagccc cgccctcggg ctggaagctt
    14821 atgcccgtga aaaactgccg gaagaggacc cgcgtcgcca tctgaaattc gcctgcggcg
    14881 atgtcaatac aacactcata cagacccggg cgggcagaag catttctgta gttttcaata
    14941 cctgcaatcc acgaccatac acgcgcatca acctgataca ggggctcaaa ggcattgtcg
    15001 agggttaccc tgaccgggtt tatgttgaag gttccagtcc cgaacacacc tgggaggaca
    15061 tggaagacta ttgggaacgg tatgaacccg ttctctggcg cgaactgggc aaagatcttt
    15121 ccggcttcgg tcacggcggt atggattatc tggaagatta tcgccttata aagtgtttac
    15181 gggaaggaaa gcgtccggac ctggatgtat acgatggcgc atcctggagc tgcattgccg
    15241 atcttagtga gaagagtgtg gcacaaaaga gtgcgccgat agaaattccg gatttcacgc
    15301 ggggtgcctg gaagtcgcga cctgccttgc ctgtccccga agcataacct cctttcctgt
    15361 attttgggtt ttacttttac tgtacacaac agcaggaaag ctatacagct ttgcatgcaa
    15421 cactatccgg aaaaagattc tttaaagaga gcaggtgttt taactttcac ctacaaatat
    15481 gctataattg ggtctgcttg ttctaacagc gttccgctgc aacttctgta aacggggcat
    15541 acgcaatcgc agcttcccca acaaacaaga caaaacgaaa tgccaccccc tcctcttaat
    15601 cccgggaaac atttctgtcc gttgaccgta gtattaagaa gatgtcatca gcacaaaccg
    15661 atcttaagga gcagtatggt atgaaaccat ctgatattgg cgctttattg ttcgagcatg
    15721 aaaatgtgtc agaagtctat gtgacacgct tcgaactgac ggataaaaaa gaaacgcttt
    15781 gcgccttcgt tgttccgact gccagtgaag gactcactga aaaagaactg catgagtact
    15841 gcgcccagcg catgcctggc aacacgcagg aaatccggat taaaatcatt gaagaaattc
    15901 cgcgaaatcc tatgggcact gtggagcggg acaaactcag gatctatgcg gagcaggcgt
    15961 cggcaaaaga agttgcctga cggctctgtc tgcttgtagc gctagccttt gctgtaatcg
    16021 ttacttggcg cataataata acggctgaaa tgatcacttt atcacccaag gagaaaccga
    16081 tgaaaatcag aacactcagt gtgcttctcg ctggctcatg tcttgccctt gcatgcagca
    16141 cgattcatgc gcaggacgtc gccgaaggac aatggatcaa cctttttgac ggtgaaagcc
    16201 tttacggatg gactccggtc ggtgacggca actgggaggt gtctgacgga gttctttcct
    16261 taacccaggg caacagcggc tttcttgcaa cgctggcaca gttttccgat tttgatctgt
    16321 ctgtcaccat gcgggtcacc ggaatgggaa cagccggcat cgcttttcgt gcacctcttt
    16381 ccggatatgc gcaggagcag ggcggcgccg cagtgaatct ggttgccgga gaagataatg
    16441 cggcattttc cacggtgaac atccgggcac tgggcaaaga agtgactgct caggtcaacg
    16501 atatcgatgt tccgacagcg gtttccaaca caaaaggaca tatcgccatt cagtttcata
    16561 agtatgaccg cttcaaaaaa ggtccgaaac ttgaaatcaa agaagtgaaa cttcgccccc
    16621 ttgccatgac ctctctcttc aatggagaga atctggatgg atggaatatt attcccgatc
    16681 gcaaatcagt cttctccgtc aatgacggtg cgctcaatat caaagacgga aacggccaga
    16741 ttgaaacaac cggtgtttat cggaatttcg ttctgcagct ggatatctat tccaatggcg
    16801 accacttgaa cagcggtgtt ttcttccgga cgcctccggg agttttctgg gtcggctatg
    16861 aagcccagat tagaaatcag tggacccgtg atgaccgaag caaaccggtc gatttcggca
    16921 caggcggtct ttacggatta aagcctgccc ggaaagtggt cagcaatgat cgcgaatggt
    16981 tcaccatgac cgttgcctgc tgcgataacc attacgccgt gtgggtgaat ggctatcaga
    17041 ccgctgattt taccgacaca cgccctgttt cggaagatgc caacggcaaa aacggctatg
    17101 tcagcactgc cggaacaatc aacctgcagg gacatgaccc gacaactgat ctctctttca
    17161 aaaatattca tattcagcct ctcgactgac aacgacgctt tctgtaattt ttagggcgga
    17221 tgtgcataac atccgccttt ttttatccct gcccggtaag gatgccgcaa tttgatgcag
    17281 cctgttcctt gcaagagacg ccctttttat gtttcactga aagcattgtt cttcccgggt
    17341 tacagcatac gaggatttgc catgcgttcc gtatttttca gtatctgctg cttttctctt
    17401 tttacagcct gcttttcgct gagtgcacag caatcggata actggcgcat gccctacgaa
    17461 ggagcagacg ttacaggcga cgacgtactg gcattctggc gttttgacga taccgacgac
    17521 agtgtgccgg acagctccgg aaaaggacat catggcaaac tggacggcgc agtcctcagc
    17581 agcgaaggaa aatttggcgg cagccttcaa tcatttccgg gctggccggc ggatgacaag
    17641 cggcatgggg cagtgatacc cgccagcccg tccctttccc cctccggcgc cttcactctg
    17701 gaactatggc tgtgccccga cgaatttccg gaaggctatg gagagtgctt tttaatcgat
    17761 aaaaaatatg tcgctcacac agattaccaa tggattcttg atcagccgga ctcaggcggc
    17821 atgcagcgta tgcggcttac cctcggattc ggcgaaacgt cggagacctg gtggtcacca
    17881 gaaagcatct ctctgaaacc gggcgtgtgg aaccatcttg ctgtcaccta tgacggtgct
    17941 ggaaagacaa cctttcacct gaacgggcgg tcattgggaa gtctgacaaa accgggacgg
    18001 ggcagtatgc aagccggtac caacccgctg gttctgggag accggatagg cagttatcac
    18061 catggctttc cgggccgcat tgatgaaata cggctgaccc ggggtgtccg tgaatttcag
    18121 ccctgcctga ttacgccggc atatgaacgc tccgtataca ggcgttttga agcaccgccg
    18181 acgctgagct accgcatcac caacatcgga aaatcggatc ttcccgttgt tnnnnnnnnn
    18241 ngacagggat gcgctccttt cccgggaaat ttcactggcc gccggtgaaa ctgcgcttct
    18301 tgatatcccc tttgacacga agcttcgacc tgatgcttat gcaatgcgta ttgcctacag
    18361 cattacggaa ggcgcagaag tttttgaaaa cgaactgtat tttcctgtaa aaatcacacc
    18421 tcgccagcct tctgcgcgca tgccggtagt catgtggggc atcggcggcg taaaagaagt
    18481 tgaaaaagca cttcccacgc tgaaagatat tggctttacc cattgtcttg gactccgctg
    18541 cgattttgaa cgtatccggg aagccgattc ccctgttccc gccgtgacag aaaaagaata
    18601 tgccgacagc tgcgccatgc tggatctggc tctggaaaat gatctgggta ttattatcgg
    18661 catcagcccc ggacactggc tcagtgaacg ggaggagaca gagcggctcg ataaagacgg
    18721 caagccttac agccggcgca atgtctgctg caactttccc gaagcgccag cctttgcgga
    18781 gaatgtagga cgctctgttg cccggacctt cggctctttt cccgctttta ccgctgcgat
    18841 gattgataca gaggtacgtg acggtacaca actctgtttt cacgaccatg acagagactt
    18901 gtacagacag gcgacaggtc tggaatttcc cgctattgcc gttaataaaa gcggtgtatc
    18961 ttatagcgat ctgccggatt ttccgcagga tcgtgttatt gaaaacgaca atgaactgct
    19021 ccgtttctac cggtggttct ggaaggaagg agacggctgg aacgcatttc attccgccgt
    19081 gtgcaaaggc ttgaaacggg acgaccgccc tgatttctgg acctattttg atccggctgt
    19141 acgggtgcca cccctctggg gcagtggcgg cagtgtggat tatctgtccc actggaccta
    19201 ttcctaccct gatcctttgc gtatgggact cgcagccgat gaactcttcg ccatggcggc
    19261 aggcggtccg gcgggacagc aggtcatgaa aatgacacag atcatctggt accgatcgca
    19321 aacggcgccg ctaactgaag gaggtcctca aggtccccgt tccgcctggg aagactacga
    19381 cccggatgcc gcctacatca ccattgcgcc cgcccatctt cgggaagcct tctggcataa
    19441 aatcgcacga cctgtcaaag ggatcatgta ccacgggtgg cagtcgctgg ttgaaacaga
    19501 aggaaaatct gtctatcggt acactcatcc ggaaacacgc ttcgcccttc aggatctgat
    19561 agagaccgtg gtgaaaccgc taggcccgac actgatgcag ttgccggaag caccctccga
    19621 cgtggctttt ctcgagagtt tcacttctac catcttcgca ggaagaggca cctatgggtg
    19681 gggcggttcc cgtgccgccg atgtttatca catgcttctc tatgcccaga tgcagccccg
    19741 tatcatttac gaagagactc tgctgacaga agggcttgac aactttcggg ttctcgttct
    19801 tgccgactgc gacgtactcc cccgtgaggt ggtcgagcag attcaggcat accagaagaa
    19861 aggcggaatc atagtcggag atgatcgcct ctgccctgct atccgccctg acattgtggt
    19921 tcaggtggaa acacgctcgg gaaaagccga tgaagataag gggcgcattc tggcgggggc
    19981 agcccgcctc gctgcgcaat tggcacccgt ctaccggcct gcaataatct cctccgaccc
    20041 ggaagtgctt ccgtacaagc gcagtttcgg cccggcggat tatgtttttg ccattaacga
    20101 caaaagagaa ttcggcagct atgtgggaca gcacggtctt gtcatggaaa acggggtacc
    20161 cgctcaggca gcatttacgc tcagccgcaa tgaaggtttt gtctatgatc tggtaagaca
    20221 ccggcaggtg gacgcctctt cggaagacgg gcatctgcgc attcccataa ctctggaacc
    20281 cggcggcggg cgcatgtatc ttgtatgtga aaataccatc gaccaggtac agataacctg
    20341 tgcagaccat gcatccagag gggaagaaat atctttcagt atttccgtgt gcgacagtac
    20401 agggaaagcg gcagacgctc tgatcccgct ggaactgcgg attaccgatc cggacgggcg
    20461 tcccgcggag ttttccggtt tttacggtgc accttccggc agtctgaatc tgactgctgc
    20521 catagcagcc aatgacacgc ccggtatgtg gacgctggaa gtccgtgaac tggcatcaaa
    20581 ccgtcagggc ttccattttt tccgtgtaga ctgacccggg caccggacat tcttaaatca
    20641 tacagcaata agcgacagga ctatgcagtt tttatctgaa tcaatcgtac agatcattgt
    20701 ttttcttgca ggcacggcgg gaagctacgg cattgccatc ctgctttttg ccgtggccct
    20761 taaagcgctt cttcttccgg tcactttata cgggatgaaa tggcaggagc gttttcaggc
    20821 tgcattcaaa tcggcaaaag aggagatagc cggtctgtca ataactgatg cagcgcaacg
    20881 ggatgacgaa attctgaata tttatcaggc gcacggcatc aatttcacca atcagtgcaa
    20941 agcgctgctg cctctgctga ttcagctgcc gctgctggtg gctttttacc gaaccatgat
    21001 tcgctgggaa ggtctggcgg agcactcctt tctctggatc agaaatcttc aggaggtgga
    21061 tgctctgatt cctctcggca tttcgcttcc ctggctgggt tccagtgtaa acctgctccc
    21121 ttttctcctc tttttagtca atgcctcaga ggtcattctt ttccgggatg aaacaaccgg
    21181 catcaaatcc ttttctttac ccctcatttt cttctttctt ttttatcctt tcccttcttc
    21241 ctgcatgctt ttctggatta tgctgaacgt ggtgcatatc ccggagcgtt ggtattacgt
    21301 caagtttcgt tcccccgtct cagatgcttc cggcacttca tcctcttcag cctgagttgt
    21361 actggaggca tccagcccgg atgccttccc cttttttatg taccttgttc tcagcgtata
    21421 catgccggta aatgacattc ctgtaaggac aagaataaac aggaaaagcg aaaacgcaag
    21481 agagcgccag gtcaggacag gattaaacac gaattcaact ttgtgtgtac ccggcggtac
    21541 aaccactcct ttaaaacgct cattcacttt cagaagcggt gccggatctc cgtcaatctt
    21601 tacggaccat ccagggtacc aggaatccag aaagctgaga atacggggct cctccagagg
    21661 tcctatctcc acaagcgcac gattggcgga gcgcccggca atatggatca gcccgtcctc
    21721 atcctccaga tctgagcgca gcagtaaacg ggtgaactga aaaggatctc tgcttttatt
    21781 ggtcactgta atttttgccg tcatctcagg aaagtcaggc aacggcactt cgg
//
LOCUS       DAOPWB010000045        21546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2430_length_21546_cov_5.540691, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000045 DAOPWB010000000
VERSION     DAOPWB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 21546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21546
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2430_length_21546_cov_5.540691"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..813)
                     /gene="radC"
                     /locus_tag="PKN92_05940"
     CDS             complement(106..813)
                     /gene="radC"
                     /locus_tag="PKN92_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNZ48369.1"
                     /translation="MKTNDTIMPSDRPAIDVEGLPRERLEKVGAAALTDAELLAVILS
                     GGSDEQKALEQARLLLDSFSILQRMGNATVSEIQYVSGIRKEQAIAIKAAFELGKRMA
                     EYKRHPGTSIQSAKDVADMLMPEFRTYETERLICLEMSTKNEVLARHDISMGGLDGTS
                     AHPRDIFCTLVRNRASAMIIVHNHPSGNAQPSQADIQISQKLCKGAELLGIRFLDHII
                     IGDGSFVSLKDQGLLKG"
     gene            complement(862..1668)
                     /gene="trpA"
                     /locus_tag="PKN92_05945"
     CDS             complement(862..1668)
                     /gene="trpA"
                     /locus_tag="PKN92_05945"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNZ48370.1"
                     /translation="MNRIAQRLAALKEQQAAAFIPFITGGDPDLSGSEDIAVTLAEQG
                     ADIIEYGVPYSDPVGDGIVIQEASLRALAQGVTLRKILASVERIRKRSDVPLMLFSYL
                     NPILSYGVERFARDAAAAGADGVLCVDLPPDDAEDYRNALKANNLESIFLAAPTSTDA
                     RLQFIGSATGSFVYYISRLGVTGEQAALSTGLAAAVARVREQCGKPVAVGFGISTPEQ
                     AHEVAGAAEGVVVGSAIVRLIAQKAGAPDMLRTVAEFARHLAEAIKKGNI"
     gene            complement(1670..2905)
                     /gene="trpB"
                     /locus_tag="PKN92_05950"
     CDS             complement(1670..2905)
                     /gene="trpB"
                     /locus_tag="PKN92_05950"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNZ48371.1"
                     /translation="MNESVSLSKRQEQPPMPDARGRYGVFGGKYVPETLMYALDQLEE
                     AFLNARQDAAFQKELTQLQQQYVGRPTPLYFAGRLTRHLGGARIYFKREDLAHTGAHK
                     INNALGQCLLARSMGKPRVIAETGAGQHGVATATAAALLGLQCDVYMGAEDMERQRLN
                     VFRMQLMGTRVIPVTSGSRTLKDAINEALRDWAAQVERTHYVLGTVHGPHPFPLICRE
                     FQSIIGKEAHEQILKAEGRLPDMMIACVGGGSNALGLFHPFLKEEDVRLIGVEAGGIS
                     LVPGMHAARFAGGTPGMLQGAMSYVLQDENGLIGRTHSVSAGLDYASVGPEHAWLHDS
                     GRVHYTHASDQEALDAFQLCSRLEGILPALESAHAVAEVIKQAPALSPEKIIVMNMSG
                     RGDKDVEQAARFILAGEEA"
     gene            complement(2853..3479)
                     /locus_tag="PKN92_05955"
     CDS             complement(2853..3479)
                     /locus_tag="PKN92_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNZ48372.1"
                     /translation="MTLRIKICGITTLEDALAACEGGADALGFVLAPEAKKRSRYIEP
                     EAAAAIIGKLPPFITTVAVVVNETEERLKQLLKVFDMLQLHGEESPEICRHLGRYAFK
                     AFRPGPGFTADLLRTWPGDCCLLDSWVPGDRGGTGVTGDWAMAKQIAAFKKLILAGGL
                     TPENVGDAVRAVRPYAVDASGSLEASPGKKDHERIRQFIKAARAAAHA"
     gene            complement(3463..4245)
                     /gene="trpC"
                     /locus_tag="PKN92_05960"
     CDS             complement(3463..4245)
                     /gene="trpC"
                     /locus_tag="PKN92_05960"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNZ48373.1"
                     /translation="MILDEIVNHKKEEILLRKTATSLRELEEQFAGMPAPPDFRAALR
                     KPGISVIAEIKRRSPSRGDILPGVDAVEIAVVYEQAGARALSILVDNKYFGGSLADLS
                     KVGNHTGLPCLCKEFIIDPYQIYEARAAGAAAVLLIVRILTDNALRSFLSEIEGLGMA
                     ALVETHNEEEVKRALDAGASIIGINNRDLDTLEVDIETTFRMKSLVPGGNILVSESGI
                     KTRKEVKLLEASGVDAILVGESLLTSRNIREKLESLLHDPED"
     gene            complement(4409..5236)
                     /gene="menB"
                     /locus_tag="PKN92_05965"
     CDS             complement(4409..5236)
                     /gene="menB"
                     /locus_tag="PKN92_05965"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNZ48374.1"
                     /translation="MTSIIHWQPAGTYQEILYAKAEGIAKITINRPHRHNAFTPLTND
                     EISDALRDARFDSSVGVIILTGMGERAFCSGGDQKIRGEAGYVDGASNQERLNVLDLH
                     RQIRTCPKPVIAMVAGYAIGGGNILAMVCDLTIAADNAVFGQTGPRVGSFDAGYGSAH
                     LARMVGQKKAREIWFLCRQYNAQQALEMGLVNTVVPLAELEKETVQWCREIMRNSPTA
                     LRFIKAAMNADEDGQAGLMELAGNATSLFYRSEEGQEGRNAFLEKRDPDFSRFPRNP"
     gene            complement(5249..6361)
                     /locus_tag="PKN92_05970"
     CDS             complement(5249..6361)
                     /locus_tag="PKN92_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48375.1"
                     /translation="MKKNCFLLMAATVLLVSGCSARRPLAHSDILQQYERPAVSLSIP
                     QEKITVAVSPVRQTLQIGGTMTAVLGAGISALQDGHYARRIEEVLGEYDFGKVLADRL
                     TAALEQVFPSPAKAIGAPGSTAGFHSIQAARKARLQGLQKRGFDAVLDLESLYGIYGA
                     EGWLAIRLKGELIDLKTGKRLWRNDITAYTLDLYEGRRWRDPMERLSPNLMSPRFSSD
                     RDAVEQWLRNDGEALRRAFELAAAQLAEAFLCDLGAGESAGGCALLGTNAFLDGKYEA
                     SLSYFRRARALAPEDPAIANGLALALFHTGAREQALSLAESALATAPDYLPLCYNLAW
                     WYGVVLKQPEPGQRYYDQSLSLGAHPARRLKRAFMH"
     gene            complement(6600..8897)
                     /locus_tag="PKN92_05975"
     CDS             complement(6600..8897)
                     /locus_tag="PKN92_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNZ48376.1"
                     /translation="MLFFSFISPSQLFLINSCIGFFAATLIAAGLIKLAYRLRITVNA
                     RYWVYVASGLFIVYRSFDILQSYRWQAIGTFLGTSQPVDLYISNVLLVSSCLSLLIGL
                     VRALLDSDLANKTLESQNTVLIYQIHQLQRLEENARKNETQLTVLLNSISSPIFFTTV
                     DGVLLECNDTFVRLFGKGETDLKSRHLRDVLPFHLFLQGKNCAEEVAASGEMQFFEGK
                     HQDRVYECTMYPVVVYSGEISHICTLALDITEKLRAEEELRLLEAAVSNTAESIMITD
                     TEGEIKYVNPAFEIMTGYTAQETKNRPSSFLGDQSQTKVSFREMWRTLQQGQVWRGIF
                     LNKRKDGSSYEEKATISPITDEQGCITHYVAVKRDVSQEQQMEQHLIQIQKMKNLGLL
                     AGGIAHNLNNALTIILGRSELALELMAADEPARESIGVVMRTAQTSSKLIKQILTFAK
                     QNSKEAEVVKVGPLLQESIKVMRSCFPTNITIHENINALNALALVDPNELQQIVVNLL
                     ENSQHALQPDGGSIDISLDLIDLEEVTPTLTGNLPSGRYLRFTVSDTGHGMDPQVAEH
                     AFDPFYSTKDVGEGMGLGLSTVHGSVKRVGGGIRLYSSPGAGTTIEIFWPEEKMRPLK
                     PAFAVESKPDGKGISVLVVDDLQDIVEMMVVGLKSYGFQVKIFSDSASALEFMEQDPA
                     AIDIAVLDYMMPKMNGIELAKRLRAFKPDLPSVLLSGYRMDVSDGEAKEVGFVAILSK
                     PILIEELARTLVRYAKTSDQEPPVV"
     gene            complement(8851..9057)
                     /locus_tag="PKN92_05980"
     CDS             complement(8851..9057)
                     /locus_tag="PKN92_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48377.1"
                     /translation="MAKESTHPVGSGWLKYFLNRDGLMLGSGMFLELLLSISALLIRS
                     AMYSSGEAFRAFFFVYFPFPVVSY"
     gene            complement(9282..10643)
                     /locus_tag="PKN92_05985"
     CDS             complement(9282..10643)
                     /locus_tag="PKN92_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNZ48378.1"
                     /translation="MKKLLTAGFPLFVCILTVAYSAGTFAQSGGWMAGVAATDITPDE
                     PVWMAGYASRTHAAEGVLLPLKAKALALQDQQGNRGLILTADVVGVSRETSDQIFSAL
                     QKALGLERKEMVFSSSHSHSGPVVGNSLRCIYPYDDEEARKLDRYTEAFIDKIINLGK
                     QAFENIRPAILYSGNGIARFAVNRRNNQEGALSSFTALEGPQDHAVPVLKVSGEDGQL
                     YAVLFGYACHATVLDGYLWSGDYPGFAQKALEDRYPGTTALFFAGCGADMNPLPRRQI
                     PLVQQYGETLAAAVAAQLEGEMKRLDPVLKTGYSETTLDMLPPPEKEELEKQVATTSG
                     YEQRCSLQLLESLEKNGSLPVSYPYPLQAWKLGGQGFAMLSGEPVVEYAIALKESLGQ
                     DLFVMGYANDYVSYIPSERIREEGGYEGCKSQIIFGLPNCWAPGLEEKILSGMRSLVE
                     SLH"
     gene            complement(10720..11832)
                     /locus_tag="PKN92_05990"
     CDS             complement(10720..11832)
                     /locus_tag="PKN92_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNZ48379.1"
                     /translation="MYKRLTEACPGHVRAPRSRFNLANRARMEGKREAARSLYELLGE
                     EHPYTPFAAEGYYRCARMAHDAGNAVLEREGLQRAAATGIEYFHAHRARYFLAQRFPE
                     EKQTGARIQSAPGQPFLIGAPCPDETEAPLLQPLISSTPAWRRIHFLGRNGLEEGDWE
                     ALDLIMNSAPSLKKLWFPVIADAGFMRTLFQYLPGSDTDESGRIPETIRRRLDYPRAY
                     WDLVVPVAKELNLDPYLILAVARQESVFCATIMSHAGATGVMQLMPATADWLARVDSR
                     ISQSQAANLKSPGNSIRLGAVYLERMLKCSEGNVIYALASYNAGPGNCDKWRARFPQA
                     DPEDFMEAIPFTETRDYVQKVMANYAAYLSLYPPVR"
     gene            complement(11905..12768)
                     /locus_tag="PKN92_05995"
     CDS             complement(11905..12768)
                     /locus_tag="PKN92_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48380.1"
                     /translation="MLMCLTLLCCAASAAVSTGEHLFLDADAAQRAGRFADAGRLFEQ
                     CAESSEDLMPYAWSRLGQLYVLSGRGEEGLALYERVLSRFPEGPWVRLTKVRQADAFT
                     RMGRREEARQASEAAFQGLSVHPWFMSDAAWQHAENCLALPEHRREAYRFYRHIAETT
                     LLVALRTKATEFLLQSSDGEDRLYGIYGLIRSGNITDARKRWNLEKNPESGAFSKPEQ
                     TVLLDQFCWDTGMPFEERLRRIEDLLRQESGKFEYRVWLMLVLREQALAGNTAAAEEF
                     AALLVRHFPEG"
     gene            complement(12841..13224)
                     /locus_tag="PKN92_06000"
     CDS             complement(12841..13224)
                     /locus_tag="PKN92_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNZ48381.1"
                     /translation="MWQFFRKERKPWLLAEDLAARHLKKRGYAILHRNLWLDRFELDI
                     VAQKGDTVVFAEVRSRSDASSTAPQETIQQTKRRHLREAARRYISRYGEGDCYYRFDV
                     IAIVMPPGEKPVVTHFENAFGMEEW"
     gene            complement(13208..14053)
                     /locus_tag="PKN92_06005"
     CDS             complement(13208..14053)
                     /locus_tag="PKN92_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNZ48382.1"
                     /translation="MTDLSVESVENSGAGDTGSDAGAFSLDSMRLQLDQFEGPFEVLL
                     YLIKEQEIDIFDIPILQVTRQYLSFLDMLRTEQLDVAGDFLILAATLIQIKSRMILPA
                     EMEQDEEEDLEEEDPRLDLVIKLLEYRKFRDLAHLLGTMEERQGDRFGRRVKPVYIPD
                     ENEEDAFEVSLYDLMKSIRGMLRFLMGEIQHRVTLEEASVDEKIARLENLLEMRESVS
                     WSDLRQECRSPLEQVCFLLAILEMCRMHRLRVRQYASFGEIRLFAHDGALPESPEPEE
                     NVAVL"
     gene            14246..15625
                     /locus_tag="PKN92_06010"
     CDS             14246..15625
                     /locus_tag="PKN92_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNZ48383.1"
                     /translation="MLYAFLFCLSPLLQSTDAQVPAGSASYSRSVMDYGAKGDGLSDN
                     TAAFQQALNEAAAASGGTVEVPAGRFRFEASLTLPANVTLKGTFHYAPAHAGIRDKKD
                     SELPVFGSVLEPVGGAGSEEGSPFITLEGSSTLQGFCIHYPDQLPDAEAPTPYPWTIA
                     MRGNNPALIDMELLNPYLGIDASRNQRALIRNIHGQPIRTGLFIDTIYDIGRVENVHW
                     NPWWTINTPVYQWQKEHGTGFLFGKTDWHYVLNTFCFGYNVGYQFIKTENGATNGNFL
                     GIGADDCHTALLVEDSEPMGILITNGEFVAFRGPNPTMIRVEKTHRGTVRFNNCAFWG
                     PGCRNALIDGVGTVGFSNCTFMQWGHQQKDDKWVPADLPSIEVVGGSVVLSDNEFLEN
                     KAQIKLGKDVERAIVTDNLMNGKVRIECDAKKNRVHIHGNLGSPEDSGFRRSLHSRKD
                     FRAMRLKEK"
     gene            15735..16088
                     /locus_tag="PKN92_06015"
     CDS             15735..16088
                     /locus_tag="PKN92_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNZ48384.1"
                     /translation="MPKLYTILLLCASNLFMTYAWYGHLKDMKGRFLPWVILLAWGVA
                     FFEYCLQVPANRIGHTQFSLGQLKVMQEIISVGIFALFAVVYMREPLRLDFLWASLCL
                     AAAAWFMFRNAGIAG"
     gene            complement(16057..17004)
                     /gene="miaA"
                     /locus_tag="PKN92_06020"
     CDS             complement(16057..17004)
                     /gene="miaA"
                     /locus_tag="PKN92_06020"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNZ48385.1"
                     /translation="MKCAVAVVGPTGSGKTALALEVAERLRTEIISADSMQFYRGMEI
                     GCAAPSAAEQQRVHHHFVSFLNPDEDMAAGKYEGLARRRAEKLLQCNALPVLCGGSGL
                     YINAFKDGLFPGPARDQSIRDRLRARAREEGTEALYAQLQEVDPDYAAQLTSANDLVR
                     IVRALEVHELTGRPFSLWHQAHQHQADKWEVLQVAPDWDREVLYARINHRVQEMIDAG
                     WVDETQRLIASGYEKDIHRLKALGYREIAAYLRGESSLEEAVEAARMHHRRYAKKQLT
                     WFRADKGIHWLPCCPASGIAAMADQVMDLISRRFPHYGT"
     gene            complement(17001..18980)
                     /gene="mutL"
                     /locus_tag="PKN92_06025"
     CDS             complement(17001..18980)
                     /gene="mutL"
                     /locus_tag="PKN92_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNZ48386.1"
                     /translation="MIKTMLEKSDIAPIRILPDTVANKIAAGEVVERPASVVKELLEN
                     ALDAGATRIIVRLVAAGRRLIEVQDNGHGMSEENALLAVERHATSKIREAQDLDSILS
                     LGFRGEALASISAVSRFLLITRREGDESATQLRIDGGILREVCRTGAPVGTRISVARL
                     YFNTPVRAKFLKGITTELNHCIDVVQRHAFACAGVGFQLFHNERLLLDVPEHASLRDR
                     TALIWGLSFVQHMVDLTGEQDGFSFHGLIGSPELNRASRSHQLFYVNRRPVSNPSMLY
                     GFQRAYQGLLTVGRHPVGIVLVDMNPRLVDVNIHPAKREIRFRDEKLAHDALQQVVRT
                     CLEKYQKQESSAGKSFVKEPFPDRESLKESRAFEVSTESEKERADFAPELPSLRKEPP
                     APHRSEDSEAYPSGVHAEPFCAPPEAEAASPDDVPDAVIPLVVPEPAVPATGLETPRQ
                     EEFESLVPQGYFEAIEQLGDAPMQLFDTYLLVPEDKRLLIIDQHALHERLNYDALLEQ
                     LAKPEPPLQQIAVPVVFQVTPAQVPLLETYRSVFASIGIEVEPFGETTFQIGAVCPLY
                     EESKVPDVLFALLDHLGRQTLFDPESVRNELLRTASRACKASIKAGDRLSIEERRSLL
                     AGFRKLRPPYTCPHGRPIIVELTRYQMERSFRRIQ"
     gene            complement(19054..19575)
                     /locus_tag="PKN92_06030"
     CDS             complement(19054..19575)
                     /locus_tag="PKN92_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNZ48387.1"
                     /translation="MDTRITNVVIAGLGGQGVIRAANILAETAFRAGLDVKQGEIHGM
                     SQRGGSVTSDIRFGEKVYSPMVPDGCADYVLVMHPTQEDNNRHHLKEGGNFISVTRLL
                     GEGQSKLSDLDSDDSTPVTERNCNIALLGILSVFLSFDDALWEAAICANLPARVHADN
                     LAVFAYGKQLAVR"
     gene            complement(19577..21172)
                     /locus_tag="PKN92_06035"
     CDS             complement(19577..21172)
                     /locus_tag="PKN92_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ48388.1"
                     /translation="MTKDLEPQLLSGNQAVALAALHLGVSLGVGYPGTPSTEILENFS
                     LIGGRAQWSPNEKVALEVGIGVAYGGGRTLVTMKHVGVNVAADPLFTVAYTGVRGGLV
                     LVSADDPGMGSSQNEQDNRRFAVAASVMMLEPSDAQESYDFLFEAFRLSEKWQLPVLF
                     RMTTRVCHSRGIVRPHAPAIPDMDPGFVRDPRAYVMIPGYARLAHKRMRQKMNEIQQW
                     NESADLNRIYEGDPSLGIVTSGVSFLHTREAAPEASVLKLGMTYPLPIEKIRAFAKTV
                     DRLLVIEENDPYLVEALRTAGIPCEGKAEMYRFGELNITRVKRIVTGDLTDETPPPPG
                     KPPQLCQGCPHRMAFAVMKKFNCIIAGDIGCYTLSVLPPFETLDTCVCMGASLGVGLG
                     LRHTLPAEEARRVVSVIGDSTFAHSGLTGVVEMVYNPPETGHVLVILDNGTTAMTGMQ
                     EHPGTGRSLDHQQTGKLVFENVIRSMGVPNVHVVNRPQDFEAVFRGSLAKNELSVIIL
                     RKVCFLAEPKLAQYEAKTRSSKD"
BASE COUNT         5090 a         6287 c         5232 g         4937 t
ORIGIN      
        1 cggaagatag taacgcagac cttcctgcca tccgctgaag ggagcatcca tgacaataat
       61 ttcctttcaa ggcacagcac agccgccagt aaatgagaat tccgtctagc ctttcagcag
      121 cccctgatct ttcagactta caaaagagcc gtcaccgatg atgatatgat caagaaaacg
      181 tatgcccagc aattccgcgc ctttgcagag cttttgggaa atctgtatat ccgcctggct
      241 gggctgtgca ttgccgctcg gatggttatg cacaataatc attgcggaag cacggttccg
      301 caccagtgtg cagaaaatat cacggggatg cgcacttgtt ccgtccagac cgcccatact
      361 gatgtcatgg cgggcaagca cttcgttctt ggtggacatt tccagacaaa tcagccgttc
      421 cgtctcgtag gtccggaatt ccggcatgag catatcggct acatccttcg cactttgaat
      481 agaggtgccg ggatgccgct tatattcagc catgcgtttt cccagctcaa aggccgcttt
      541 aattgcgatt gcctgttctt ttcggatgcc tgatacatac tgtatttcgc tcaccgttgc
      601 attgcccata cgctgcagaa tagaaaagga gtccagaagc agtcttgcct gttccagcgc
      661 cttttgctca tcgcttccgc cgctcagaat cacagcgagc agctcggcat cagttaatgc
      721 tgcggctccc actttttcca ggcgctcacg gggcagccct tcgacatcaa tggctggtct
      781 gtctgagggc attatcgtat catttgtttt cataaaaggc tctgcagttg ctttcccgaa
      841 agatttctgc cagtccgtac atcagatatt gccttttttt attgcttcag caagatgccg
      901 tgcaaattcc gcaacagtcc ggagcatatc aggtgcaccc gccttttgtg ctatcagacg
      961 gacaattgcg gagccgacca caacgccttc ggctgctccg gctacttcat gcgcctgctc
     1021 cggcgtggaa atgccgaaac ccactgccac cggtttgccg cattgctccc gaacccgtgc
     1081 gactgctgcc gccagcccgg tgctgagtgc tgcctgctcg cctgttacac ccagtctgga
     1141 tatgtaataa acaaagctgc ccgtagcact gcctataaat tgcagccggg catcggtgct
     1201 tgttggcgct gcaagaaaaa ttgattcgag attgttcgct ttgagcgcat tccggtagtc
     1261 ttccgcatca tcaggcggca gatccacaca cagcacgccg tctgcacctg ccgccgccgc
     1321 atcacgggcg aaacgctcca cgccgtagct taaaatggga ttcaagtagc tgaaaagcat
     1381 cagaggcaca tcacttcttt tccggatgcg ttcaacagaa gccagaattt tcctgagcgt
     1441 aactccttgt gccagtgccc gcaaagaagc ctcctgaatc acaatgccgt cgcctacggg
     1501 atcggaatag ggcacaccgt attcaatgat atccgccccc tgttctgcca gcgtcaccgc
     1561 aatatcttcg cttccggaca aatcaggatc gccgcccgtg ataaaaggaa tgaatgccgc
     1621 cgcctgctgc tctttcagtg ccgccaagcg ctgtgctatt ctgttcatat cacgcctcct
     1681 ccccggcgag aataaaacgc gccgcctgct caacatcttt atcgcccctg ccggacatat
     1741 tcataacaat gattttttcc ggagataatg ccggagcctg cttaatcact tccgcaaccg
     1801 catgagcgct ttccagtgcc ggaagaattc cttcgaggcg gctgcacaat tgaaacgcat
     1861 ccaatgcctc ctgatcgctc gcatgggtat aatgaaccct gccactatca tgcagccagg
     1921 catgctccgg tccgacgctg gcataatcca gtccggcgga cacactgtgc gtcctgccta
     1981 tcagaccgtt ctcatcctga agcacataac tcatagcccc ctgaagcatt cccggcgttc
     2041 ctccggcaaa gcgtgcggca tgcatgcccg gcacaagaga gattcccccc gcttccacac
     2101 cgatcaggcg tacatcctct tcttttaaaa acggatgaaa cagccccagc gcgttggaac
     2161 caccgccaac acaggcaatc atcatatcgg gcaaacgccc ctcggctttc agaatctgct
     2221 catgtgcttc ttttccgata atggattgaa attcgcggca aatcagaggg aaaggatgag
     2281 gcccgtgcac tgtacccagc acataatgcg tacgctccac ctgagcagcc cagtcgcgca
     2341 gcgcttcgtt gatggcatct ttcagtgtcc gggatcctga agtgacagga atcacccgcg
     2401 ttcccatcag ctgcatgcgg aacacattca aacgctgccg ctccatatct tccgcaccca
     2461 tgtagacatc gcactgaagc ccgagcagcg ctgcggcggt ggcagtggcg acaccgtgct
     2521 gccctgcccc tgtttccgca atgacccgcg gcttccccat gcttctcgcc aggagacact
     2581 gaccgagggc attattaatt ttatgggcac ccgtatgcgc aagatcttcc cgcttgaaat
     2641 aaatgcgggc accgcccagg tgtcgggtca gcctcccggc aaaataaagc ggcgtcggcc
     2701 gccccacata ctgctgctgg agctgtgtaa gttctttctg aaaagcggca tcctgccggg
     2761 cattcaaaaa cgcctcttcc aactgatcca gtgcgtacat cagcgtttcc ggcacatatt
     2821 tcccgccgaa aacgccatat ctgccccggg catcaggcat gggcggctgc tcttgccgct
     2881 ttgataaact gacggattcg ttcatgatcc ttctttcccg gtgatgcttc aagagatcct
     2941 gaagcgtcga ccgcataagg gcgcactgcc ctgacggcat cacccacatt ttccggcgtc
     3001 aacccgccgg cgagtatcag ctttttaaat gctgcaatct gctttgccat tgcccagtcg
     3061 cctgtaacac cggtaccgcc ccggtctccg ggcacccagc tgtcgagaag gcagcagtct
     3121 ccgggccagg ttctgagcag atccgctgtg aacccgggac ccgggcgaaa ggccttaaag
     3181 gcatatctgc cgagatgacg gcatatctcc ggagattctt ctccgtgcaa ctgcaacata
     3241 tcgaagacct tcagcagctg cttcagtctc tcttcggtct cattgaccac aacggcaact
     3301 gttgtaataa aaggcggcag ttttccgata atggcagcgg cggcttccgg ctcaatataa
     3361 cgactgcgct tttttgcttc cggtgccagc acaaagccca gtgcatcggc accgccttca
     3421 cacgccgcca gcgcatcttc cagagttgta atgccgcaga ttttaatcct cagggtcatg
     3481 gagcagcgat tccagttttt cgcgaatatt gcggcttgtg aggagagact caccaaccag
     3541 tatggcatcc acacccgacg cctccagaag tttgacttcc ttccgggttt taatgccgct
     3601 ttcactcacc agaatattgc cgccgggcac cagacttttc atacggaacg tcgtctcaat
     3661 atccacttcc agtgtgtcaa gatcccggtt attgatgccg ataatcgacg caccggcatc
     3721 cagcgcccgc ttcacttcct cctcattgtg tgtttccacc agtgccgcca tccccagccc
     3781 ctctatctcc gagagaaagg aacgcagcgc attgtctgta agaatccgga caataagcag
     3841 caccgccgct gcacctgcag cccgggcctc ataaatctga tagggatcga taatgaactc
     3901 tttgcacagg cagggcagcc ctgtatgatt gccgactttg ctcagatccg ccagcgagcc
     3961 gccaaaatat ttattgtcga cgagaataga gagcgccctt gcgcctgcct gctcgtacac
     4021 caccgcaatc tccacagcat ccaccccggg caggatatct ccccgggagg gggatctgcg
     4081 ttttatttct gcaatcacgc tgataccggg cttgcgcagg gcggcacgaa agtccggcgg
     4141 cgccggcata ccggcaaact gctcttccag ctcccgcagg gatgtagctg ttttgcgcag
     4201 caggatctct tcttttttat gattgacaat ttcatcaaga atcatggcag tctgtacttt
     4261 tcctgttttc ccgaagcgat gtgaatctgt gtccggagtg gagggttaag acgtaacggt
     4321 gcgccttgtg tgctctcgtt ataatactgg aatatgactt tcgcttcaac cgttcatgat
     4381 gattcgtccg aaagcagctc cggttatttc atgggtttct ggggaagcgg ctgaagtcgg
     4441 gatctctttt ttcaagaaag gcgtttcgcc cctcctgccc ttcttccgag cgataaaaga
     4501 ggctggtggc gtttcctgca agttccatca gtcccgcctg accgtcttca tccgcattca
     4561 ttgccgcttt gataaagcgt aaagccgtcg gagaattgcg cataatctca cggcaccact
     4621 gcaccgtctc tttttccagt tccgccagcg gaaccactgt gttcacaagc cccatctcca
     4681 acgcctgctg tgcgttgtac tggcggcaga ggaaccagat ttcccgggct tttttctgac
     4741 ccaccatgcg ggcaagatgt gccgacccgt acccggcatc aaaagaaccg accctcggac
     4801 cggtctgacc gaagacggcg ttgtctgcgg caatggtcag atcacagacc atggcaagga
     4861 tatttccgcc gcctatcgca tagccggcaa ccatggcaat gacaggcttc gggcaggtgc
     4921 ggatttgccg atgcagatcc agcacgttca gccgttcctg attggaagca ccatcaacat
     4981 accctgcctc tccccttatt ttctgatcgc cgccgctgca aaaggcacgc tcgcccatac
     5041 cggtcaggat aatcacaccc acagacgaat caaagcgcgc gtcccggagg gcgtcactga
     5101 tttcatcatt agtcagaggc gtgaaagcat tgtgccgatg gggccggtta attgtgattt
     5161 tggctattcc ttccgctttc gcataaagaa tttcctgata agtgcctgcc ggctgccagt
     5221 gaatgatgga agtcatgaca tattcctgtt agtgcataaa cgcacgtttt agacggcgcg
     5281 cagggtgcgc accaagcgaa agagactggt catagtaacg ctgccccggc tccggttgtt
     5341 tcagcacgac accataccac caggcgagat tatagcagag aggaagataa tcgggtgccg
     5401 ttgcaagcgc gctttctgca agagacagcg cctgctcacg ggcgccggtg tgaaacaatg
     5461 cgagcgccag cccgtttgca atggcaggat cttcaggtgc caaagcccgg gcacgccgga
     5521 aataggagag gcttgcctca tatttaccgt caagaaaagc gttggttccc aaaagcgcac
     5581 aaccgccggc actttcaccg gcgcccagat cacacaaaaa ggcttctgcg agctgcgccg
     5641 ccgccaattc aaaagcccgg cgcagcgctt ccccgtcatt tcgaagccat tgttccacgg
     5701 cgtcccgatc ggagctgaac cggggcgaca tcaggtttgg actaagccgt tccatcggat
     5761 cccgccagcg ccttccttca tacaggtcaa gggtataggc agtgatatca ttgcgccata
     5821 aacgcttccc cgttttgaga tcaatcagct cacccttcag cctgatcgcc agccacccct
     5881 cggctccgta aatgccatag agactttcaa gatccagcac cgcatcgaaa ccgcgcttct
     5941 gcagcccctg cagccgggct tttcttgccg cctgaatact gtgaaatccg gcggtcgagc
     6001 cgggcgcacc gatcgctttt gccggagaag ggaacacctg ttccagtgca gctgtgagcc
     6061 ggtcggcaag gactttaccg aagtcatatt cgccgagcac ttcttcaata cgccgtgcat
     6121 agtgcccgtc ctgcagggca ctgataccgg cacccagcac tgcagtcatc gtgcccccga
     6181 tctgcagggt ctgccgtaca ggcgacacgg caaccgttat cttttcctga gggatactga
     6241 gactgacagc aggccgctca tactgctgaa gaatatctga gtgtgccaga gggcgccggg
     6301 cggaacatcc tgacaccagg agcactgttg ctgccatcag aagaaaacag ttttttttca
     6361 cgattatttt ttccctgaat gttttgcaga aatcgaaagt atctggtgcc gggatcagcg
     6421 taaaagccgc caacggcacg aaaaaagatg ctgcaaatga gtgtttttac agggaattat
     6481 agcacaggcg caacaatacg cctatgccgt ggcgccagcc cccggatatg atcagaagaa
     6541 tcagtgtatt ctcctttgta ccgcccggag aaaaaaggaa cgatttcagg ttttaaaaat
     6601 cagacgacag gcggttcctg atcagaggtc tttgcatacc gaaccagagt gcgggcaagt
     6661 tcctcaatca gaataggctt ggacagaatg gcaacaaagc caacctcctt cgcctctcca
     6721 tcacttacat ccatgcgata accgctgagc agaacagaag gcagatccgg tttgaacgct
     6781 cgcagtcttt tagccagctc tatgccgttc attttaggca tcatataatc cagtaccgca
     6841 atatcaatgg ctgccggatc ctgctccata aattccagcg cactggcact gtccgaaaat
     6901 atcttgacct ggaatccgta acttttcagg cctaccacca tcatttccac aatatcctga
     6961 agatcatcca caaccaggac ggaaattcct ttcccatccg gctttgactc aactgcaaaa
     7021 gcaggtttaa gagggcgcat cttttcttca ggccagaaaa tttctatcgt agtgcccgca
     7081 cccggtgaac tgtacagcct gatgcctccg ccaacgcgct tcacactccc atgcacagta
     7141 gaaaggccga gtcccatccc ttcgcccaca tctttagtgc tgtaaaaagg atcaaaggcg
     7201 tgttccgcca cctgcggatc cataccatgc cctgtgtcgg acactgtaaa ccgcaaatat
     7261 cttcccgaag gtaaattgcc ggtcagggta ggcgtcactt cctccagatc aatgagatcc
     7321 agtgaaatat ctatgctgcc gccgtcaggc tgaagggcat gctgcgaatt ttcaagaaga
     7381 ttaacgacaa tctgctgcag ttcattgggg tccaccagtg caagtgcatt caaagcattt
     7441 atgttctcgt gaatggtgat gtttgtaggg aagcaggaac gcatgacctt gatagattcc
     7501 tgaagcaaag gtcccacttt taccacttcc gcttccttgg aattttgctt tgcaaatgtc
     7561 aatatctgtt tgatcagttt cgaggaggtc tgagctgtcc gcataaccac tcctatgctt
     7621 tcccgcgcag gttcatctgc agccattaat tccagtgcca gttcgctccg ccccagaatg
     7681 atggtcaagg cattattcag attatgagca atgccgcccg caagaaggcc cagatttttc
     7741 atcttctgaa tctgaatcag gtgctgctcc atttgctgtt cctgagaaac atcgcgctta
     7801 acagccacat agtgggtgat gcacccctgc tcatcagtta tgggcgaaat tgttgccttt
     7861 tcttcataag aagaaccgtc tttacgctta tttaaaaata tgcctcgcca tacctgaccc
     7921 tgctgcaacg tacgccacat ctcacgaaag gatactttag tctgcgactg atcccccaaa
     7981 aaagaagacg gtcggttttt tgtttcctgt gctgtgtacc cggtcatgat ttcgaaggcg
     8041 ggattgacgt atttaatctc tccttccgtg tcggtaatca taatgctttc cgctgtattg
     8101 gatacagccg cttccagaag acgcagttcc tcttccgcac gcaatttctc ggtaatatcc
     8161 agcgccagcg tacaaatatg gctgatctct ccggagtaca ccaccacagg atacatcgta
     8221 cattcataga ctcgatcctg gtgttttccc tcaaagaact gcatttcgcc tgaagcagcc
     8281 acctcctcag cacagttttt tccctgcaga aaaagatgaa atggaagcac atcacgcaaa
     8341 tgcctggact tcagatccgt ttcccctttg ccgaaaagcc ttacaaacgt atcgttgcac
     8401 tccagaagaa caccgtccac agtcgtaaaa aagatgggag aagaaatgga gttcagcaga
     8461 acagtcaatt gcgtttcgtt ttttcgggcg ttttcctcca gacgttgaag ctgatgaatc
     8521 tgatagatga gtaccgtatt ctggctttca agcgttttat tggcaaggtc cgaatcaagc
     8581 agtgcgcgaa ccagcccgat cagcagcgac aaacaactcg aaacaagcag tacattggaa
     8641 atataaaggt ctacgggctg ggaagttcct agaaatgtcc caattgcttg ccagcggtag
     8701 gactggagaa tatcaaagga acgatacaca atgaacaagc cactggcaac atagacccag
     8761 tatcgcgcgt tcactgtaat tcgaagcctg tatgccagtt ttatcagacc ggcagcgatt
     8821 aacgtcgcgg caaaaaaacc gatacagctg ttaataagaa acaactggga aggggaaata
     8881 aacgaaaaaa aaagcacgaa aggcttctcc cgatgagtac atggccgaac gaatcagaag
     8941 cgctgaaatg gaaaggagca gttccagaaa catgccgctg cccagcatca gcccgtccct
     9001 gttcaggaag tacttcagcc acccgctccc gacaggatgt gtactttctt ttgccataac
     9061 ccgatccttt cctgccgacg attctcagcc aggctgagat acaacttatc gacctattga
     9121 aaacaacatc tgtattttct tatttcatta ttcacgttaa ttagtttact gatattataa
     9181 cacatatacc aaacgtctat atcatattta ttattttctt tgcggacaca caacggtgcg
     9241 cagttgtgaa tactaagaat taccctgttc caaacaaaag gctagtgaag agattcaaca
     9301 agggagcgca tgccggaaag gatcttttcc tccagtcccg gcgcccagca gttgggcagt
     9361 ccgaaaataa tctgggactt gcagccttcg tacccgcctt cttctctgat acgctcggaa
     9421 ggaatatagg acacatagtc gttggcatac cccatgacaa acagatcctg ccccagtgac
     9481 tctttcaggg caatggcata ttccacgacc ggctcgccgg aaagcatggc aaagccctgc
     9541 ccgcccagtt tccacgcctg aaggggatag ggatagctga cgggcaaact cccgttcttt
     9601 tcgagcgatt cgagaagttg gagactgcac ctctgttcgt acccgcttgt cgtcgccacc
     9661 tgcttttcca gttcctcttt ctcaggcgga ggcagcatat ccagcgtggt ttcactgtac
     9721 cccgtcttta gtaccggatc caggcgtttc atttcacctt ccagctgtgc agccacagcg
     9781 gcagcgaggg tttctccgta ttgctgcaca agcggaatct gccggcgggg cagcggattc
     9841 atgtctgcac cgcacccggc aaaaaacagc gccgttgttc cgggatagcg atcttccagc
     9901 gctttctgag caaaaccggg ataatcgccc gaccacagat acccatcgag cacggtggca
     9961 tgacaggcat aaccgaatag gacagcatac agctgcccgt cttcaccgct gactttcagc
    10021 acaggcacgg catgatcctg cggcccttcc agcgctgtga atgaactcag cgccccttcc
    10081 tgattgttgc gccggttgac ggcaaagcgg gcaatgccat ttccgctgta aagaatggca
    10141 ggccggatgt tttcgaaggc ttgttttccc agattaatta ttttgtcaat aaacgcttcc
    10201 gtataacggt ccagcttgcg cgcctcttcg tcatcataag gataaatgca ccgaagagaa
    10261 tttccgacaa ccggcccgct gtgactgtgt gaggaactga acaccatttc cttccgttca
    10321 agtccgagcg ctttctgcaa tgcagaaaaa atctgatccg acgtttcccg cgacacgccg
    10381 accacatcgg cagttaagat caacccgcgg ttgccctgct gatcctgcag tgccagcgct
    10441 ttcgccttca gcggaagcag cacaccctct gccgcatgcg ttcgggacgc ataccccgcc
    10501 atccagaccg gttcatccgg cgtaatatct gtcgccgcca ctccggccat ccaaccgcct
    10561 gattgcgcaa aagtacctgc gctgtacgca acagtcagga tgcatacaaa aagaggaaat
    10621 ccggcagtta acagcttctt catgaaatgc tccaatccgt ggtttagtct ggtcattgtg
    10681 cttattattg ccaccgggac agagagatgc aaataccgct cagcgcacag gcgggtacag
    10741 tgagaggtat gccgcatagt tcgccattac tttctgcaca tagtctcttg tttctgtgaa
    10801 gggaatggct tccataaaat cttccggatc tgcctgcgga aaacgggcgc gccatttatc
    10861 acaattaccc ggcccggcat tatatgatgc cagcgcataa atcacattcc cctccgaaca
    10921 cttcagcatg cgctccagat aaacagcgcc caggcgaatg gaattgcccg gtgacttcag
    10981 attagccgcc tgactctgtg aaatacggct atccacccgg gcaagccagt cggctgtcgc
    11041 cggcatcagc tgcatcacgc ccgtggcgcc ggcgtgagac atgatggtgg cgcaaaatac
    11101 gctttcctga cgcgctactg ccagaatgag atatggatcc agattcagtt ccttggcaac
    11161 aggcaccaca agatcccagt aagcccgggg atagtccagc ctgcgcctga tcgtttcagg
    11221 tatcctgccg gactcatccg tatcggagcc gggaagatac tggaagagtg tgcgcatgaa
    11281 gcccgcatcg gcgatgactg gaaaccacag tttttttaac gaaggcgcac tgttcataat
    11341 cagatccagc gcctcccagt ccccttcctc caacccattt ctgcccagaa agtggatgcg
    11401 ccgccaggcg ggagttgatg aaatcagcgg ctgaagcaaa ggcgcctccg tttcgtcagg
    11461 acagggggcg ccgattaaaa aaggctgtcc cggtgcgctc tgaatccggg caccggtctg
    11521 cttttcttcc gggaaacgct gtgcgagaaa atagcgcgct ctgtgggcat gaaaatactc
    11581 tatacctgtc gcagccgccc gttgtaatcc ctcacgctcc agcactgcat tccctgcatc
    11641 atgagccatc cgggcacatc ggtaatagcc ttccgccgca aaaggtgtgt agggatgttc
    11701 ctcgccgagc agttcatata aggaacgggc agcctctctt tttccttcca tccgggctct
    11761 gttggctaga ttaaaccggg atcggggagc acgaacatgc cccggacacg cttcagtaag
    11821 gcgtttgtac atgcggtcgg caccagcagt atcccctgcc ttttcaaagc gttcggaaag
    11881 ccaccagtat ccgtctcccc cgtctcagcc ctcaggaaag tgccggacga gcaaagcggc
    11941 aaattcttca gctgctgccg tgttcccggc aagtgcctgc tcgcgaagaa caagcatcag
    12001 ccacactctg tattcgaact tgccggactc ctgccgaaga agatcttcaa tacggcgaag
    12061 ccgttcttca aaaggcattc ctgtgtccca gcagaactga tccagcagta ccgtctgctc
    12121 cggctttgaa aaagcaccag attccggatt tttctccaga ttccagcgtt tccgggcatc
    12181 ggtgatatta cctgatcgaa taagcccgta aattccatac agtcggtctt caccatcgga
    12241 cgactgcagc agaaactcag tggcttttgt tcgcagcgcc acaagcagtg tcgtttcagc
    12301 aatgtgacga taaaaccggt atgcctctct ccggtgttcc ggaagtgcca gacagttttc
    12361 tgcatgctgc caggcggcgt cgctcataaa ccacggatga accgacaaac cctgaaaagc
    12421 cgcttcagaa gcctgacgcg cctcttcccg acgccccatt ctggtaaagg catcggcctg
    12481 acgcactttt gtcagacgca cccagggccc ttccggaaag cggctcagga cacgttcgta
    12541 taacgcaagc ccttcttctc ctctgccgct cagcacatac aactgcccca gccgtgacca
    12601 ggcatagggc atcagatctt ccgaactctc cgcgcattgt tcaaaaagcc gtcccgcatc
    12661 agcaaagcgc cctgctctct gcgcagcatc ggcatccaga aacagatgct ctcctgtgga
    12721 tacggcggca gatgccgcac aacaaagcag ggtcaggcac ataagcatgc aagtcttctc
    12781 ctttggcttg agtcgccgca atcctgaatc cttctctgca acatcagcgg gaacacatcc
    12841 tcaccactcc tccatcccga atgcattttc aaaatgcgtc accaccggct tttctccggg
    12901 aggcatcaca atggcgatta catcaaaacg ataatagcag tccccttcac cataccggga
    12961 aatataacgg cgcgccgcct ctctgaggtg acggcgtttg gtctgttgaa tcgtctcctg
    13021 aggtgcggtt gaactggcat cactccggga acgcacttcg gcaaaaacca ctgtatcccc
    13081 tttttgcgcc acaatatcca gttcaaagcg atcaagccag agattgcggt gaagaatagc
    13141 gtaaccacgc ttcttcaggt gccgtgccgc cagatcctcc gcaagcagcc agggcttacg
    13201 ttctttccta aagaactgcc acattttctt ccggctccgg gctctcagga agcgcgccgt
    13261 cgtgggcaaa gagacgtatc tcgccgaaag aggcgtattg ccgcactctc aggcgatgca
    13321 tccggcacat ctcaagaatg gcgagaagaa aacagacctg ctccagcggg ctgcggcatt
    13381 cctgacgcag atccgaccag gaaacgctct cccgcatctc cagcagattt tccagccgtg
    13441 cgattttttc atcaacagac gcctcttcca gcgtgacacg gtgctgtatc tctcccataa
    13501 gaaaacggag catgccgcga atggatttca tgaggtcata caggctgact tcaaaagcat
    13561 cctcttcatt ttcgtcggga atatagaccg gtttgacccg tctgccgaag cgatccccct
    13621 gcctctcctc catggtgccc agaagatgtg ccagatccct gaattttctg tattccagca
    13681 atttaatgac cagatcaagg cggggatctt cttcttccag atcctcttct tcgtcctgct
    13741 ccatttcagc aggcaggatc atgcgcgatt tgatctgaat cagcgtagcc gccagaataa
    13801 gaaaatcgcc ggcaacgtcc agttgttccg tacggagcat gtcaagaaaa ctgagatatt
    13861 gccgggttac ttgaagaatg ggaatatcaa agatatctat ttcctgctct ttaatcagat
    13921 aaagaagcac ttcaaaaggg ccctcaaact gatccagctg caggcgcatg gaatccaggc
    13981 tgaaggcacc ggcatcggag ccggtatcac ctgccccgct attttcaaca gattcaacag
    14041 acaaatcagt catgagctct cgtttccact gatgtcacgc aaacatttat tgatcagaat
    14101 acttctctcc cgccccttca tcataccgtt ttaagccggt aaaacacaac cgcccgggac
    14161 acggcacatt tcgctcaaca ttgattttat cggtgcggta caggtatgtt tatgacgttt
    14221 tactgcaacc aaaggaatac ccgccatgct ctacgctttt ctgttctgcc tctctccact
    14281 gctgcaaagt accgatgcac aagttcccgc cggatccgcc tcttattccc ggtctgtgat
    14341 ggactatggc gccaaggggg acggcctttc ggataatacc gccgcctttc agcaggcact
    14401 taacgaggca gctgccgctt ccggaggcac cgttgaagta cctgccggcc gatttcgttt
    14461 tgaagcctcg ctgacgcttc ccgccaatgt aacacttaag ggcacttttc actatgcgcc
    14521 tgcccatgcc ggaatccggg ataaaaaaga ctcagagctt cctgtattcg gcagtgttct
    14581 ggaacccgtg ggcggcgcag gcagtgaaga gggcagccct tttattaccc tggaaggcag
    14641 cagcacactg cagggcttct gcatccacta ccccgatcag cttcctgatg cggaagctcc
    14701 cacaccgtac ccctggacca ttgccatgcg tggcaacaac ccggcactga tcgatatgga
    14761 actgctgaac ccttatctgg gcattgatgc atccaggaac cagcgcgcac tcatccgtaa
    14821 tattcacggt cagcccatcc gcacaggact cttcatagac accatctacg acatcggcag
    14881 agtagaaaat gtgcactgga acccctggtg gactatcaat acgcctgtct accaatggca
    14941 aaaagaacac ggcacggggt tcctcttcgg taaaacggac tggcattatg tcctgaatac
    15001 cttctgtttc gggtacaacg tgggctatca gttcatcaaa acggaaaacg gtgccaccaa
    15061 cggcaatttt ctgggcatcg gcgccgacga ctgtcacacc gccctgctgg tggaagacag
    15121 cgagcctatg ggcattctca ttacgaacgg tgaatttgta gcatttcgcg ggcccaaccc
    15181 gaccatgatc cgggtggaaa aaacacaccg gggcaccgta cgctttaata attgcgcctt
    15241 ctggggaccc ggttgccgca atgccctcat cgacggagtc ggcacagtcg gcttcagcaa
    15301 ttgcaccttc atgcagtggg gtcaccagca gaaagatgac aaatgggttc ccgccgatct
    15361 cccttccatc gaagtggtgg gtggcagtgt ggtactttca gacaacgagt ttctggaaaa
    15421 caaagcccag atcaaactgg gaaaagatgt ggaacgcgcc attgttacag acaatctgat
    15481 gaatggtaaa gtacgcatcg agtgtgacgc gaagaaaaat cgcgtccaca ttcacggcaa
    15541 cctcggctcg cccgaagatt ccggattcag aaggtcgctt cacagcagga aggatttccg
    15601 ggcaatgcgg ttaaaagaaa aataatatct tttccggcag agccggactc cgaacagccg
    15661 tttttttttg ttgaaactcc tctcttgacg agtactttca ttaatcacat cttaaactgc
    15721 agcacaggtg tcttatgccc aaactctaca ccatactcct tttatgcgcc tccaatcttt
    15781 tcatgaccta tgcctggtac gggcatctga aggacatgaa aggtcgtttt cttccctggg
    15841 ttatcttgct tgcgtggggt gtcgcttttt ttgaatattg ccttcaggtt ccggcaaatc
    15901 gcatcgggca cacacaattt tcgctgggtc aattgaaggt catgcaggag attatcagcg
    15961 tgggcatttt tgcgcttttc gcggtggtct acatgcgcga accgctgcgc ctcgatttcc
    16021 tctgggcgtc cctctgcctt gctgctgcag cctggttcat gttccgtaat gcgggaatcg
    16081 ccggctgatc agatccatca cctgatccgc cattgccgca ataccggaag cgggacagca
    16141 tggaagccag tgaatccctt tgtccgcacg gaaccaagtc agctgttttt tcgcataccg
    16201 tctgtgatgc atgcgggcgg cttccaccgc ttcctccagg ctgctctctc cccggagata
    16261 cgcagcaatt tctctgtagc ccagcgcttt cagacggtga atatcctttt cgtagcccga
    16321 cgcaataagc ctctgagtct cgtccaccca tccggcatcg atcatctcct gcacgcgatg
    16381 attgatccgt gcatacagca cttcccggtc ccagtcaggt gccacctgta atacttccca
    16441 tttatccgcc tggtgctgat gtgcctgatg ccacagagaa aaggggcgcc ccgtcagctc
    16501 atgcacctcc agcgcccgga caatacgcac cagatcgttg gcgcttgtca gctgcgccgc
    16561 atagtccgga tcgacttcct gaagctgcgc gtagagcgct tctgtcccct cctcacgggc
    16621 acgcgcacga agccgatcgc gaatgctctg atcccgtgca ggtccgggaa acagaccatc
    16681 cttgaaagca ttgatataaa gacctgagcc gccgcagagc acgggcagcg cattgcactg
    16741 cagcagtttt tccgcccgtc gacgtgcaag cccttcatat ttgcctgccg ccatatcttc
    16801 gtccggattg agaaaagaaa cgaaatgatg gtgaacccgt tgctgctccg ctgctgaagg
    16861 agcggcacag ccgatctcca tgccccgata aaactgcatg gagtcagcag aaataatttc
    16921 tgtccgaagc cgctccgcca cttcgagtgc cagagcagtc ttgcccgaac cggtgggtcc
    16981 caccacagca acagcgcact tcactggatt ctcctgaaac tgcgttccat ctgataacgt
    17041 gtcagctcca caatgatggg acgcccgtgg gggcaggtat aaggcgggcg cagttttcgg
    17101 aatccggcaa ggaggctgcg ccgctcttct atgctcaatc gatcgcctgc cttgatggac
    17161 gccttgcagg cacgcgatgc tgtacggagc aattcattgc gcacggactc aggatcaaaa
    17221 agagtctggc gacccaaatg atccagcaat gcaaaaagga catcgggaac tttgctttct
    17281 tcatagagcg gacaaaccgc acctatctgg aaggttgtct ctccgaaagg ttccacttca
    17341 ataccgatgg acgcaaagac agagcggtag gtttcaagca gtggcacctg cgcaggggtc
    17401 acctggaaca ctacaggcac agcaatctgc tgcaaaggcg gttccggttt agccagctgc
    17461 tccagaaggg catcatagtt cagacgttcg tggagggcgt gctggtctat gatcaggagg
    17521 cgcttgtctt caggcacgag cagataggtg tcaaagagct gcataggcgc atcgcccagc
    17581 tgctcaattg cttcaaaata accctgcgga accaggcttt caaattcttc ctgtctcggt
    17641 gtttccaatc cggttgccgg gacagcaggc tccggtacta caagcggaat tacggcatcg
    17701 ggaacatcgt ccggagaagc tgcctccgct tccggcggcg cacagaaggg ctcagcatgc
    17761 accccggaag gatatgcttc gctgtcttcc gatctgtgag gcgcgggcgg ctctttccgg
    17821 agtgacggca attccggggc aaaatctgca cgctctttct ctgattctgt cgacacctca
    17881 aaagcccggg attctttcag gctctctcta tccggaaacg gttctttgac aaaggatttt
    17941 cccgctgagc tctcttgctt ttggtatttc tccaggcagg tacgcaccac ctgctgcagg
    18001 gcatcatgag caagtttctc atcacgaaaa cgtatttcac gcttcgccgg atggatattc
    18061 acatccacca accggggatt catatccacc agaacaatcc ctacaggatg ccgccccacc
    18121 gtcaggaggc cctgataggc gcgctgaaaa ccgtagagca tggaaggatt ggaaacaggc
    18181 ctcctgttaa cataaaaaag ctgatgtgac cggcttgccc tgttcagctc gggactgcct
    18241 atcagtccgt gaaaagaaaa accatcctgt tctcccgtca aatcaaccat atgctgcaca
    18301 aaagacaagc cccagatcag agcagtgcga tcccggagcg aagcgtgctc gggcacatcg
    18361 agaaggagac gctcattatg aaacagctga aacccgaccc cggcacaggc aaaagcatgc
    18421 cgctgcacca cgtcaataca atggttgagc tccgtcgtga ttcctttgag aaatttggcg
    18481 cgaaccggcg tgttaaaata gaggcgggcg accgatatgc gggtgcccac aggcgctcct
    18541 gtgcggcata cttcacgcag gattccgccg tcgatgcgca gctgtgtggc gctttcatct
    18601 ccttcccggc gggtaatcag cagaaaccgg gatacggctg atatgctcgc cagtgcctcg
    18661 ccacggaatc ccagcgaaag gatggagtcc agatcctgcg cttcccggat tttactggtg
    18721 gcatggcgtt ctacggcaag cagcgcattt tcttcagaca tgccatgtcc gttatcctgc
    18781 acttcaatca gcctgcggcc tgccgccaca agccggacaa taatccgcgt cgctcctgca
    18841 tcgagcgcgt tttcaagcag ctccttcact accgaagcag ggcgttccac cacctcaccg
    18901 gcagcgattt tattggcgac cgtgtcgggc agaattcgga tcggggctat gtcggatttt
    18961 tccagcatgg tcttaatcat accataaaaa aggcctctcc tttttcagga gaagcctgtg
    19021 cattcaatgg acaaaaagtg aatacctgcc tttttagcgc accgccagct gcttgccata
    19081 agcaaaaaca gccagattat cggcatgcac ccgggcaggc aggttggcgc atatggccgc
    19141 ttcccagaga gcatcatcaa aagaaagaaa tacactcagt atgccgagaa gcgctatatt
    19201 gcagttccgc tctgtcaccg gcgtggaatc atcgctgtcc agatcggaaa gcttgctttg
    19261 cccttctccc aaaagccggg tcacgctgat aaaattaccg ccttctttga gatgatggcg
    19321 gttattgtcc tcctgagtag gatgcatcac cagcacataa tccgcacagc cgtccggaac
    19381 catggggctg tataccttct caccgaaacg gatatcactg gttaccgacc cgccacgctg
    19441 actcatcccg tgtatctcgc cctgcttcac atccagcccg gcacgaaatg ccgtttccgc
    19501 aagaatattg gcggcacgga tcacaccctg accgcccagc cctgcaatca caacattagt
    19561 tatacgcgta tccatgtcag tccttgctgc ttcgggtttt cgcttcatat tgagcgagtt
    19621 taggttcggc aagaaaacag accttcctga ggatgatcac ggaaagttca ttcttcgcca
    19681 gagatccccg gaaaaccgct tcgaaatcct gcggtctgtt caccacatgc acattaggca
    19741 cacccatgga acggatcacg ttctcaaaga cgagtttgcc cgtctgctga tgatccagag
    19801 agcggcctgt gcccggatgc tcctgcatgc ctgtcatcgc cgtggtgccg ttatccagaa
    19861 tcacaagaac atgccccgtt tccggcggat tgtaaaccat ctcaacaaca ccggtaagac
    19921 cgctgtgggc gaatgtgctg tcaccgatga cactgacaac acgacgtgct tcttcggcag
    19981 gaagcgtgtg acggagccca agcccgacgc ccaggctggc acccatgcag acacaggtgt
    20041 ccagcgtttc aaagggcggc agcacgctca gcgtgtaaca gccgatatca cctgcaataa
    20101 tgcagttgaa ttttttcatc acggcaaaag ccatccgatg cgggcagccc tgacagagtt
    20161 ggggtggctt gcccggaggg ggcggcgttt catccgtgag atccccggta acaatccgct
    20221 tcacccgggt aatgttcagc tcgccaaaac gatacatctc cgccttgcct tcgcagggga
    20281 tgcccgccgt ccgcaaggct tccaccagat agggatcgtt ttcctcgatc accagcaggc
    20341 gatccactgt cttcgcaaaa gcccggatct tttcgatcgg aagcggatac gtcatcccca
    20401 gttttaaaac agaagcttcc ggcgctgctt cgcgggtatg cagaaaagaa acgcccgatg
    20461 tgacaatacc cagcgacggg tcgccctcat aaatacggtt aagatcagca ctttcgttcc
    20521 actgctgaat ctcattcatt ttctgacgca tgcgcttatg ggcaaggcgg gcatagcccg
    20581 gaatcatgac ataagcgcgg ggatcgcgga caaaaccggg atccatatcc gggattgccg
    20641 gtgcgtgagg tctcacaatc cctctggaat ggcatacacg ggtggtcatc cgaaacagga
    20701 caggaagctg ccatttctcg gaaagccgga aagcctcgaa aagaaaatca tagctttcct
    20761 gggcatccga cggttccagc atcatgacgc tggcggcaac ggcgaaacga cggttatcct
    20821 gctcattctg actggaaccc atgcccggat catcagcaga cacaagaaca agtccgcccc
    20881 gaacccctgt gtaggcgaca gtgaacagcg gatcggctgc cacattgacg cccacatgct
    20941 tcatggtcac cagtgtccgg ccaccgccgt aggcaacacc gatccccact tccagcgcca
    21001 ctttttcatt gggtgaccat tgtgcgcgcc ctccgataag agaaaagttt tcaagtattt
    21061 cagtggaagg cgtgccggga tagccgactc ccagcgaaac ccccagatgc agtgccgcca
    21121 gagcgactgc ctgatttcca ctcaacaact gcggttccaa gtccttggtc atgtcgggtt
    21181 ctcacagcct gatggttcag aagttcagaa aaataatgga aatgcccctg ttcaggagcc
    21241 atgttggaaa gcgctactgt aacaaatcac cccgggacac atcaactgaa ctgtgaaaaa
    21301 aggcaaggca aattcagata accggatact gctttttgca gattctttcg ctctaatttt
    21361 cagcctgcgc ctgaaggcaa aaccattgca aagcgcttat tgccgtgcta tactgattat
    21421 agggcatgca ctctttcata gccccttatt cgttttgatc acccgacagc atgttctttt
    21481 ttttataaag aggtcactta actgataagg agcactcgta atgaaacgaa aaggatttac
    21541 tcttat
//
LOCUS       DAOPWB010000046        21285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2466_length_21285_cov_6.080217, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000046 DAOPWB010000000
VERSION     DAOPWB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 21285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21285
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2466_length_21285_cov_6.080217"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2509)
                     /gene="alaS"
                     /locus_tag="PKN92_06040"
     CDS             complement(<1..2509)
                     /gene="alaS"
                     /locus_tag="PKN92_06040"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNZ48389.1"
                     /translation="MKSIDIRASYLDFFRQRGHVVEKSDRLVPDNDPTLLFTSAGMVQ
                     FKHFYTGEVAVPYRRAVTSQKCLRAGGKANDLDEVGKTSRHLTFFEMLGNFSFGDYFK
                     REAISWAWEYTREILKFPAEKVIVSVFHEDDEAAEIWEKEIGVPASRIVRLGAKDNFW
                     GPAGETGACGPCSELLYDRGVEIDPAATLENDPHERFLEFWNLVFPQFDQQMDGSRPP
                     LKNRGIDTGMGLERIAALLQNKETVFDTDGIFPIIEATLSLSSFRYEEKPVPCRVIAD
                     HARAVTFMLADGILPGNEGRGYVFRRLLRRAARFGRELGLEKPFLHQVAMHVIDMMQE
                     QYPELSQGRTRIEKTILAEEERFAGALARGMELLESVFWKMKKDGLTQVPGEELFKLH
                     DTFGFPLDLAADVAEEQGYTVDKAGFEAAMRNQRDKARSAWAGSGEQKVATVYRTLES
                     EHGATEFVGYDNLTADAVVLALVQNNRAVDVLAEGCEGEVLLNLTPFYAESGGQVGDR
                     GIIEFSEGKAEVSSVLKRAGKSFLHQVKVLSGNLHTGTQVSAQVCAAKRSRTEAHHTA
                     THLLQAALRHTLGDHVQQAGSYVDSERLRFDFTHFEAITLEKLQEIERFVNACIREAV
                     PVEISFKSIEEARKEGAMALFGEKYEERVRVVQTGDKSKELCGGCHVRNTGVIGICKI
                     LSDSAIAAGVRRIEAVCGEPCLDMLQERERQFQTVLQTLSARPDEVEKRLHGLIEENK
                     RLQREVEKWKQTAATGSGKDLMSQVKEIKGLKVLIIQLADLDAKSLRTVLDQYKDKLG
                     SGVVVLGGGGEGKVALAAGVTKDLIARISAGDIVSRLA"
     gene            complement(2595..3272)
                     /gene="lepB"
                     /locus_tag="PKN92_06045"
     CDS             complement(2595..3272)
                     /gene="lepB"
                     /locus_tag="PKN92_06045"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNZ48390.1"
                     /translation="MNADDFERITPVEEQGATPFPDSKGNDTSESRTLLNRVFFSPRI
                     GMIALLFLLGGLLYLLLGKEMRFFMVPSASMESTLFAGDMIVTFNQKAYARGDIVVMK
                     HEGEYLVKRIIGLPGDSLTIIDGALFINGKYASEPYVRESMHYYIEEPVLIPPGYFFY
                     LGDNRNLSDDSSRGFANYAEKLDAAARLSYLGVLNDIIGKVHFIYYPYNRLGNLPSYI
                     LTNTAGS"
     gene            complement(3286..4161)
                     /locus_tag="PKN92_06050"
     CDS             complement(3286..4161)
                     /locus_tag="PKN92_06050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ48391.1"
                     /translation="MLTQEQKDFYDAMNDGVYIVDRNRKILYWNKAAEEIAGFTAEEV
                     IGKSCADNILIHVTESGVQLCTGPCPIADSIQHARANQARVYLHHKEGHRVPVYVRTA
                     PFLNQANSVVGGIELFSMDAPRLQDKKSTLKSQESGQECTVTHLPNRRYLERELKTDL
                     EDLREHDYHFGIILFQIDGMDKQRASHSEEICNALIKIVGKTVAFAVRPFDVVGRWGE
                     ASFLGIFATGSMEGLDKISERVKGLVAHSKIIENGEPISVSVLTAATLAEHDDTVASI
                     VERAEALLASHQQNT"
     gene            complement(4260..5021)
                     /gene="kdsB"
                     /locus_tag="PKN92_06055"
     CDS             complement(4260..5021)
                     /gene="kdsB"
                     /locus_tag="PKN92_06055"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNZ48392.1"
                     /translation="MSEKKVAGIIPARFGSSRLPGKPLADIAGKPMIQHVYEQCSKAV
                     LLHELWIATDDERICHAVRNFNGNVMMTRPDHCCGTDRIAEAAADMDADIIVNIQGDQ
                     PFIDPGMIDQVVAPLLANPAVQIATLKKPVEEEAALHDPAVVKTVTDLTGRALYFSRS
                     LIPYPREKTNLRIYEHIGLYAYTKAVLLQITRLQPTALEKTESLEQLRWLEHGYHIQV
                     ETATDSDPLFHGFSVDTEADLLRAAELYKDRMNSF"
     gene            5305..6384
                     /locus_tag="PKN92_06060"
     CDS             5305..6384
                     /locus_tag="PKN92_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNZ48393.1"
                     /translation="MPLDFEAVLRLAVEHNASDIHLKVGSTPILRIDGKLERVSDCDV
                     LRGSDIFAIIERIASDDFLKRFQETMEMDTSYLLKDVARFRVNACIEDGKPRVVMRTV
                     PLQIKTLEELQLPPILTRLCTLKNGLALFTGPTGSGKSTSLAAMIDRINSTRTDHIVT
                     IEDPLEFIHVNKAGIVTQREVGIDTLSFSNALRASLRQDPDVILIGEMRDSETVQTAL
                     SAAETGHFVLSTLHTVDAVETLHRILEFFQENQQMQIRNQLASVLRCICSQRIMPRID
                     GEGRIVAAEILIGNRTVREFIQQCRPFKEIVKLIEDGASQYGMQTFDQSLYSLWKEKI
                     ISKETALLNATSSKDLQLRMQGLYS"
     gene            6520..6825
                     /gene="gatC"
                     /locus_tag="PKN92_06065"
     CDS             6520..6825
                     /gene="gatC"
                     /locus_tag="PKN92_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNZ48394.1"
                     /translation="MEHVTEQDIDHYASLSQFQLNDDTRKRFTKEINQILDFLQILQK
                     VDTESIAPTRHGTDRVNVFRDDVIQDPLPHAEVFKNSPLDDGVFFLVPRIIEGEDNS"
     gene            6822..8291
                     /gene="gatA"
                     /locus_tag="PKN92_06070"
     CDS             6822..8291
                     /gene="gatA"
                     /locus_tag="PKN92_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNZ48395.1"
                     /translation="MKTPWYEKTAKEIRDAVHQGEASALDIVNSFIEHADAVDGTVGA
                     YTQRWSETARTAAQRIDEKIRKGLPAGALAGVPVALKESICTSEGKTTGASKMLADYR
                     SPFDATAVTRLQQADAVFLGKVNMDELGLGSSTETSALQRTTNPWAPDRVPGGSSGGS
                     AAAVASGTCALALGTDTGSSIRQPASFCGCIGFKPTYGRISRLGIIGIASSLDHVGPL
                     ARTVEDTALALNVLCGEDKGDPTSSDRPVPDFTRSLGRDIRGLRIGVPKEYLASDLDA
                     EVRQYVEKALDQFRNAGAEIVSISLPCTGYDAAAYTVISTAEMSAGLARLDGVRYGAR
                     HPEAKSVPDVYVMSKSAGLGTEVQRRIILGTYFLSGKNRQDYFVKAQQVRTVVKKDFQ
                     NAFEQCDIIAGATTATAAFPFGSKSDNPLEMYACDQFTVAANLASHPALSIPCGKTAA
                     GLPIGLHLQARSYDEETLLGAAFYYEQHRGFEMGFPSIL"
     gene            8303..9733
                     /gene="gatB"
                     /locus_tag="PKN92_06075"
     CDS             8303..9733
                     /gene="gatB"
                     /locus_tag="PKN92_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008858880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNZ48396.1"
                     /translation="MQYEAVIGMEVHVEINCHSKVFCSCPSAFDAAPNSQVCPGCLGM
                     PGALPVLNRKMVDKSIAVGLALNCQIARSTRMDRKNYFYPDLARNYQISQDDQPLCYA
                     GYIDVDVEGRKKRIRITRAHVEEDTARNSHTQAGGQSGIDFNRSGLSLLEIVTEPDIR
                     SAQEAFAYLTSLKQLLQYLDVSDCNMEEGSLRAEANISVRPVGAEYYGTKTEVKNVAS
                     FSGTQKAIEYEVERQIEVLNSGGKVVQETRGWDADKGITVSQRLKESAHEYRYFPEPD
                     IPPIVVDEAWIEQIRKELPEFPIARKERFQSQYGLSLYDAQVLTFSRAMADFYEAIVS
                     AGADPKKAANWLMVELQALLSDAKLDISASPVSPASLAELLALIETGIINGKIAKEVL
                     VEMFKSGAAPKKIVEEKGLGQISDSKALEETIRAIIEANPGQAADYRAGKEKLFDYFV
                     GQVMKATRGKGNPQLVNEILRRELDS"
     gene            9760..10137
                     /gene="rsfS"
                     /locus_tag="PKN92_06080"
     CDS             9760..10137
                     /gene="rsfS"
                     /locus_tag="PKN92_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNZ48397.1"
                     /translation="MPNQLNEKDKLVKKVQQAAEGLLSKKALDIKAHLVSGATLIADA
                     FVFCSANSAVHLKAVANGAREWMRDGGYEILRVEGDHQSGWLILDYGDVIIHIFRAEA
                     RAFYELDKLWADTPEIPLALDIN"
     gene            10155..10994
                     /locus_tag="PKN92_06085"
     CDS             10155..10994
                     /locus_tag="PKN92_06085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ48398.1"
                     /translation="MDSQYTPDIQQLYHFKLQRILGEGGTGRVYLGVDMKKGTPVALK
                     LFRENFFRNRLHVRDLAKSVTKFKRFKNNNVVQIYDFYDGDEGRCMIMEYVDGPSMKW
                     YILNRPYNLAERVNVAIQICRGMQYLHDKGCVHHDFKPANVLFTRTGVVKIADFSLYG
                     SSFLLELIDKNIGEQITPMFVAPEFIRKEKVTAQSDIYSMGISFYMLFTSKVPFPVDN
                     IRKLYQCHLAVMPDHPSLAAPECPNAIGDIIMKMLAKRPEARFSDCDELAVALAQTQR
                     SRI"
     gene            11137..12174
                     /locus_tag="PKN92_06090"
     CDS             11137..12174
                     /locus_tag="PKN92_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48399.1"
                     /translation="MKRKLRIGIIGCGAIAQELHIPEYLDSGEAEIIALSDTDLKKAQ
                     AANERYSLNAQLFPSYKDMLKKAAPDAVSVCLPNYLHCAATCDALQAGCHVLVEKPMA
                     ASMAEARKMVAFADKYKKLLAVNQSQRLYPAHIKAKEVLDSGILGKVLHVTAMFGHAG
                     PEHWSPSGKWFFNKKQARFGAMADLGVHKADLIRFLTGKEVAEVNAFYECLEKKGATV
                     EDNFVSTLKFTDGTIGTLSASWTVKGIDANYTILHCTNGTLRVCLLPDRPLVANLVNP
                     ECEIVFKVPEPPSNTNLGWGLDTGGHFCRAIKGDEPLFCPGVEGMKSLEIIFAAEEAA
                     KKGRTVKLTHK"
     gene            12185..13279
                     /gene="aroB"
                     /locus_tag="PKN92_06095"
     CDS             12185..13279
                     /gene="aroB"
                     /locus_tag="PKN92_06095"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNZ48400.1"
                     /translation="MAQTITVSLGERSYPIHIGENLLHLIPEMLQKSGIRGSIGVITD
                     THVAELYLQQTISFLKEAAFPCVTHILPAGEESKQLSRIEEICGTFLEGRLDRASAVV
                     ALGGGVVGDIAGFAAACFMRGIPFIQVPTTIVAQVDSSVGGKTAVNHALGKNTIGCFH
                     QPCGVVIDMTVLKTLPPRELRAGCAEIIKHGVILDAGLFNYMEKEAASVLACDLKALF
                     YPVARSCEIKSAVVAEDEKEHGIRALLNFGHTFGHALETVSNYSTFLHGEAVALGMCA
                     AAELGRLLGTASPEFCCRLRACCQAYGLPVVWSELPVREALQAMKKDKKARAGTLKFI
                     VPCDFGTVEQRTDITEELAGKALESLLRES"
     gene            13304..14128
                     /locus_tag="PKN92_06100"
     CDS             13304..14128
                     /locus_tag="PKN92_06100"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48401.1"
                     /translation="MLFGGETAESYYEEGLTSSIKGDYEQALVFFHKALSLNPVFAAA
                     HHQIAKTHIRLGAFQKAIDALNQAVALMGRKTSALADLAQAYYLLGKVKEARTIFSEI
                     LQISEKDTRGVLGLAFCAFADEQWVTAINLVQHASTLGRLHFDAHFLIARAAAKTGAE
                     DIAGFHFQQALEQMNHTLEITEEQVTGYFLRGLVHASMGGLLDALRDMDSALRYIDPD
                     KNYFAYNHFFNKVEILVQKGVLLKDLNRIREAKETGEQILALAPDNQTGMMLASLS"
     gene            14150..14740
                     /locus_tag="PKN92_06105"
     CDS             14150..14740
                     /locus_tag="PKN92_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48402.1"
                     /translation="MSDVSLQLVREYFELNFFYILPHWGIVAGQTKELETGSSLLFVE
                     QQGKPNRAEPEFLLRSGDIAALHRAAVEVRAWHGGKLYSSAIGATSLFSRVASRQTHD
                     LATALFKSDNYKIVLVVSELPISEPAKKAALEKLHARGIHHVIEFRTILGDLLRQISA
                     QGNYGPSKTLQTLKLLKRYGFIHPEELRQLNLPLDS"
     gene            complement(14768..15223)
                     /locus_tag="PKN92_06110"
     CDS             complement(14768..15223)
                     /locus_tag="PKN92_06110"
                     /inference="COORDINATES: protein motif:HMM:NF016264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HNZ48403.1"
                     /translation="MKNDVQNLVTIIMQRIAASNGAVSVGDVLRAELQKDGFKEKDIN
                     VAFEFVSSGMQGKMSFDLKFPARRHLNVFESFKIQEEVMETISKLEQMGLLDPMEREA
                     LLENIQHSDGRADKDDLEYALSVMLAPKRNSEAQNALLTVSEGYTPTFH"
     gene            15400..16572
                     /gene="sfsA"
                     /locus_tag="PKN92_06115"
     CDS             15400..16572
                     /gene="sfsA"
                     /locus_tag="PKN92_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HNZ48404.1"
                     /translation="MASYTLSAFHEPLIKVRFQERPNRFLVRGIDAQKQEYLAFLPNP
                     GRLSELLLPDTCIYLIKKSPSQKERATDFTAIAVERDGVPVMLHTHLCNDMVEVLLKK
                     QKVPGLEKASIVRREVKFGKSRFDFLLQDQAGEIYLEVKSCTLFGSGIAMFPDAVTER
                     GTRHLMELAHLAETSQFRCCVLFVVQTDTARYFMPDYHTDPVFAGTMVQTKDLLRYVP
                     LPVAWNSDMSFTAGRKPLQIPWSYIQKENEDRGAYLLVLKLEKDSAVTIGALGTFTFK
                     AGYYLYVGSALGNLSARIARHKRPLKRFHWHIDYLRQVAKNVDAFPIRSSSNIECALA
                     DALRPLYETPLPRFGSSDCTCRSHLFRSSSYPVHDAEFHTVLQRFRMQKPPENTLI"
     gene            16627..17511
                     /locus_tag="PKN92_06120"
     CDS             16627..17511
                     /locus_tag="PKN92_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ48405.1"
                     /translation="MNSFTHQKPFTRPQIIAHRGASAYAPENTRSAFLKAIELGADWF
                     ELDVQLTRDGAMAVFHDRDVSRFAVKHIPIYDLTMSDLRSLDVGSWFSEEYAHERVIT
                     LEEALELASGKIGVYIELKSTVDETPRIPDMLEVVFQSKSLSAWDWRYLFDAGHRISA
                     DSMVLAQRAIDLVRKYRDQCRMVVQAFSPVIAMVFRKEAPDIPFEFLGMDLPEPPNIW
                     RDFVLFGEKIDVEGFNVCRTSLTEERFLYFQKKKKRCAVWVVDEPEEIRQFTAMGVHG
                     LISNKPDICRTELSALGY"
     gene            17580..19127
                     /locus_tag="PKN92_06125"
     CDS             17580..19127
                     /locus_tag="PKN92_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNZ48406.1"
                     /translation="MEGGFAFVDWTILFIYMGVMLLIGALVSRQQSDSQFFFLGGRSM
                     PSWAVALSVVATALSAATFIGVPQMAFEGDFTYLATNLGAMIAAFIIAFYFVPPIYAA
                     GTVTIYGYLGQRYGESARTAAGAMFLLGRLLSSGARLFMAGIGFSLMLYGETARHHLI
                     FAILLLGIVGTLYTVSGGIRAVIWTDVIQIIVMVVAALAAVAYLVSLIPLTPGQMVAE
                     LTSSQGVNKLKVLDFSLNFSNPFTFWSALFAMTIVSLSTHGVDQDMLQRVMTAKSPFH
                     GGLALIGSMAIAVPIILVFLIIGALLHLFYTRPDLMGNAFPPDVVEDTRRLFPQFVLQ
                     HLPVGLRGLTMAGLIAAAMSTFDSAINAMASSLIADLYVPLRAFLQKMKSGQKEEEHL
                     PDLAGSRFAVGLMGFLLTLFAIGAIYLQEQGGESLINFALGVMAFAQAPLLGVFCTAL
                     FTRRGNAASALTAFATGIGLVLLLQPYMLPKWAGFTLGWTWTWAIVSPVCFLICLCGS
                     PGTEEIS"
     gene            19124..20491
                     /locus_tag="PKN92_06130"
     CDS             19124..20491
                     /locus_tag="PKN92_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNZ48407.1"
                     /translation="MNNSLFPEEKPVPSSLPKKEAPLARRVAPKSLDEILGQSHILGE
                     GKLLRRAIEADRITSIILYGPPGCGKTALARVIAMRTKAVVEPLNAVLAGVTDIRRVV
                     KSAQERFTTAQRATILFLDEIHRFSKTQQDALLPHVENGLFTLIGATTENPYFSIVSP
                     LLSRSQIFEFKALSEEDIQVLLNRALTHRGRGFSGIEIQIEPEAAAYIAKFSEGDGRR
                     AINALELAILTTKPVENTICITLPIAAESIQQKLLHYDGSGDSHYDAASAFIKSMRGS
                     HPDAALFWMARMIEAGETPRFIARRICIAAAEEVGCADPMALVLANAAFQAAEFVGFP
                     EARIILAEAAVYVASAPKSNASYLAIDAALGMVREGKSIAVPDHLKDSSYQGAKKLSR
                     GIGYRYAHDFEDAYVAQDYGVPRSIFYKPTERGQEELIKKRLDTLEEIDRNAAESVQP
                     SEEPQ"
     gene            complement(20513..21025)
                     /locus_tag="PKN92_06135"
     CDS             complement(20513..21025)
                     /locus_tag="PKN92_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNZ48408.1"
                     /translation="MNDKAQSQDGTKERDMSAEGASWREYTYSNLSHSRAHYLLAIES
                     LRDGLGYARTTDVAEMLEVSRGAASMALSQLKKRGWVTEDPNRFLLLTAEGKKMTRLV
                     SRNYEVLRSFFESVLGLSSELATEEACKMEHLITVETSDLLGKFNKVLTGSPALLDKI
                     KKQMDSVSDT"
BASE COUNT         5328 a         5412 c         5135 g         5410 t
ORIGIN      
        1 gcgccagccg ggaaactatg tcgcctgcgc tgatacgggc aatcaggtct tttgtaacac
       61 cggcagccag tgcgactttc ccttctccgc cgcccccaag cacaacaacg ccggaaccca
      121 gtttgtcttt gtactgatca agcacagtac gaagactttt ggcatcaaga tcagccagtt
      181 ggatgatcag aactttgagc ccttttattt cttttacctg gctcatcaaa tcttttccgc
      241 tgccagttgc ggcagtctgt ttccactttt caacctctct ttgcagccgc ttgttttctt
      301 caataaggcc atgaagccgt ttttccactt catcagggcg cgcactgagc gtttgaagca
      361 ctgtctgaaa ctgacgttca cgttcctgaa gcatatcgag gcagggctct ccgcagactg
      421 cctctatgcg acgcactccg gcagcgattg ccgaatcgct aagaattttg cagatcccta
      481 taacgcctgt attccggaca tggcatccgc cgcagagttc cttgctttta tcgcctgtct
      541 gcacaacccg gacgcgctct tcatattttt caccgaacag tgccatggca ccttctttac
      601 gggcctcttc aatactctta aaagagattt ccaccggcac ggcctcgcgg atacatgcat
      661 tgacaaaacg ctctatctcc tgcagctttt ccagagtaat cgcttcaaag tgggtaaagt
      721 caaagcgcaa ccgttctgag tcaacatagg atccggcctg ctgaacatgg tcgcctaacg
      781 tatgcctgag cgcagcctga agcagatgag ttgccgtatg gtgggcttcg gttctgcttc
      841 tttttgctgc acatacctga gcacttacct gagtaccggt atgcaagttg ccggacagca
      901 ctttaacctg atgcagaaaa gattttcctg cgcgcttcag gacggaactc acttctgctt
      961 taccttcaga aaattcaatg attcctctgt cccctacctg cccgcccgac tcagcataaa
     1021 atggagtaag gttcagcagc acctcgcctt cacaaccttc tgcaagtaca tctacagccc
     1081 ggttgttttg caccagtgcc agaactacgg catctgccgt caagttatcg tagccgacaa
     1141 actcagtggc gccatgctca ctctcaagcg tacggtaaac cgtggcaact ttctgctctc
     1201 cgctgcctgc ccaggcacta cgggccttat cccgctgatt gcgcattgcc gcttcaaagc
     1261 ctgccttatc caccgtatat ccctgttctt cggcgacatc tgcagccaga tccagaggaa
     1321 aaccgaaggt gtcatgaagt ttgaacagtt cttcaccggg cacctgtgtc agcccgtctt
     1381 ttttcatttt ccagaagacg ctttccagaa gttccatgcc tcgtgccaga gcaccggcaa
     1441 aacgttcttc ttctgcaagg attgtttttt caatgcgtgt tcttccctga ctcagctcgg
     1501 gatactgctc ctgcatcata tcaatcacat gcatggcgac ctgatggaga aagggtttct
     1561 ccagccccag ttctctgccg aagcgtgccg cccttctgag cagccgcctg aagacatatc
     1621 cccgcccctc attgccgggt aagataccat cagcaagcat gaaggttacg gcgcgggcat
     1681 gatcggcaat cacccggcac ggtaccggtt tttcttcata acggaaggaa gagagggaca
     1741 aggtggcttc aataatggga aagatcccgt ctgtatcaaa aacagtttcc ttgttctgta
     1801 gaagtgcggc tatccgctcc agccccatgc ctgtatcaat gccacgattt ttcagaggcg
     1861 gccggctgcc gtccatctgc tgatcaaatt gcggaaacac caggttccaa aattcaagaa
     1921 agcgctcatg aggatcattt tcaagagtgg cagcaggatc aatctccacg ccgcgatcgt
     1981 acagcagttc tgaacagggc ccgcaggcac ccgtctcccc tgccggaccc cagaaattat
     2041 ctttggcacc cagacgaacg atacgtgagg caggcacgcc tatttctttt tcccagattt
     2101 cggcggcttc atcatcttcg tgaaaaacgg agacaatgac cttttccgcc ggaaatttca
     2161 gaatttcacg ggtatattcc cacgcccagg aaatggcttc gcgtttaaaa taatcgccga
     2221 aagaaaaatt gccgagcatc tcgaaaaaag taaggtgccg ggaggttttc ccgacttcgt
     2281 ccaggtcgtt agctttgcca cctgcacgaa ggcacttctg agaggtcaca gcgcggcgat
     2341 agggaacagc cacttcgccg gtgtagaaat gcttaaactg aaccatacct gcactggtga
     2401 aaagcagtgt aggatcattg tcgggcacta aacggtcact tttttcgacc acatgcccgc
     2461 gttggcggaa aaaatcaaga taactggctc gtatgtcgat gcttttcatt gtagttctcc
     2521 ggaaaattga gtggggatcc ctataatggt accaaactca accgtcccgc tgcaccctcg
     2581 ttcagaacag gaagttatga ccctgccgta ttggtcagaa tataagaagg caaatttccc
     2641 aggcggttat agggatagta gataaagtgt acttttccaa tgatatcgtt taaaacgccc
     2701 agatacgaca ggcgggcagc ggcatccagt ttctccgcat aattggcaaa accgcgactg
     2761 ctgtcatcac tgaggtttct gttgtcacct aaataaaaga aatagcccgg cgggatcagc
     2821 acaggctcct caatgtaata atgcatggat tctcgaacat aaggttccga tgcatatttg
     2881 ccgttaataa acagggctcc gtctattatg gtgagagaat cgccgggcag tccgatgatt
     2941 cttttaacca gatactcacc ctcgtgcttc atcacaacaa tgtcgccccg ggcataggct
     3001 ttctgattga aggtaacaat catatctccg gcaaaaaggg tggactccat agatgcggaa
     3061 ggcaccataa aaaagcgcat ctccttgcca agcaacaggt aaagcaaccc gccaaggaga
     3121 aacaaaaggg cgatcatgcc gatccgcgga ctgaaaaaaa cacgattcag gagcgtacgg
     3181 gactctgaag tatcgttgcc tttactgtcg ggaaatggtg tcgccccctg ttcctcaaca
     3241 ggagttatcc tttcaaaatc gtcagcgttc acttacggta tcgtctcagg tgttttgttg
     3301 gtgggaggct aaaagagctt ctgcccgctc aacgatgctt gcaaccgtat catcatgttc
     3361 agccagggtg gctgcggtga gaacagacac ggaaatcggc tctccatttt ctataatttt
     3421 tgaatgggca acaagtccct taacccgctc acttatttta tccagccctt ccatggaacc
     3481 ggtagcgaag atacccaaaa aggaagcttc cccccaacgt cccaccacat cgaaaggacg
     3541 cacggcaaag gcgacagtct ttcctacaat tttaataagg gcattgcaga tttcctcact
     3601 gtgtgaagcc cgctgcttat ccatgccatc aatttggaag aggataattc caaagtggta
     3661 atcatgttca cgcagatcct caagatcggt tttcagttct ctttcaagat accttctgtt
     3721 gggcaggtgt gtaacagtgc attcctgtcc gctttcctgc gattttaagg tgcttttttt
     3781 atcctgcaga cgcggggcat ccatggaaaa aagttcaatg ccgcccacta cagaatttgc
     3841 ctggtttaaa aatggagctg tgcgtacata tacggggacg cgatgccctt ctttgtggtg
     3901 cagatacacg cgcgcctgat ttgcccgggc atgctgaata ctgtctgcaa tggggcaagg
     3961 tccggtgcac agctgcacac cgctctccgt cacatgaata agaatattgt ctgcacagga
     4021 ttttccgata acttcctctg ctgtaaagcc ggcgatctcc tcggctgctt tattccaata
     4081 cagtattttc ctgttgcgat cgacaatata aaccccgtca ttcatcgcat catagaaatc
     4141 tttctgttcc tgtgtaagca taaaattcct ttcctttaca aagcctcact tttacttaaa
     4201 ctgcataaag agcaacaaat acgctggcac cctatgcaat acaaattatg aacatttttt
     4261 caaaaagaat tcatccgatc cttatacagt tcggcagcac gcaagagatc ggcttccgtg
     4321 tctacactga aaccgtgaaa aagagggtcg ctgtccgtcg ctgtttcaac ctgtatatgg
     4381 taaccatgct ccagccagcg aagctgctca agagactctg ttttttcaag tgcagtcggc
     4441 tgcaggcggg taatttgcag aagcaccgct tttgtatacg catacaatcc gatatgctcg
     4501 tagatccgga gattggtttt ctcacgggga taaggaatga gagaacgtga aaaataaaga
     4561 gcccgtccgg taagatcagt gactgttttt acaaccgccg gatcatgcag tgctgcttcc
     4621 tcctcaacag gcttcttcag ggtcgcaatt tgaactgccg ggtttgcaag aagaggagcg
     4681 acaacttgat caatcatccc gggatcgata aaaggctggt cgccctgaat atttacaatg
     4741 atgtcggcat ccatatcggc agcagcttcg gcaatgcgat cggtaccgca gcaatgatcc
     4801 gggcgcgtca tcatgacatt cccgttgaaa ttccttacgg cgtggcaaat tctctcgtca
     4861 tcggttgcaa tccacaactc atggagcagg acggctttgc tgcactgctc ataaacatgc
     4921 tggatcatgg gctttccggc aatgtccgcc agaggctttc ccggcagtct gctggatccg
     4981 aaccgggcag gaatgatccc tgccactttt ttttcactca tatcaactgg ctttccaatt
     5041 aaaaatggat tcagagatta ttgcatctcc ggcacggaga tgcttatact tggagacaat
     5101 gattagttta ccggaaagta atgggataaa caaccagtta ccggggaaat ttcattggtt
     5161 tcccgcacca atcacattct tatcagcacc ggttttatgc aaagacgctg tccgccaaat
     5221 taacagaaaa caacagaaac ggtctttcgt ttataatggc ttgcgttaca aaactgttga
     5281 acttactatt cgggagtttg tgctatgcct ttagattttg aagcagtgct gcgccttgcc
     5341 gttgaacaca acgcctccga tatccacttg aaggtcggaa gcaccccaat tcttcgcata
     5401 gatggaaaat tggaacgcgt ctcagattgc gatgtcctgc gcggaagcga tatttttgcc
     5461 atcatagagc gtatcgccag cgacgatttc ctgaaacgct ttcaagagac aatggaaatg
     5521 gatacatcct atcttttgaa ggatgttgcc cgcttccgtg taaatgcatg cattgaagac
     5581 ggaaaaccac gtgtcgtcat gcgtacggtc ccgcttcaaa ttaaaacgct ggaagaactg
     5641 caactgcctc cgatcctgac acggctgtgc acccttaaga acggactggc tctttttact
     5701 ggtcccacag ggagcggaaa atcaacgtct cttgccgcca tgattgatcg aatcaattct
     5761 acgcgtaccg atcatattgt gaccattgag gatccgcttg aatttattca tgtgaataaa
     5821 gcaggcatag ttacccagcg tgaagttgga attgacacac tttctttcag caatgcactg
     5881 cgtgcttcgc tccgtcagga tcccgatgtg attcttatag gggaaatgcg tgacagtgaa
     5941 actgtgcaga ccgctctttc tgccgccgaa acggggcact ttgtactgtc tacgctgcac
     6001 acagtcgatg ctgtagaaac gctgcaccga attctcgaat tcttccagga aaaccagcaa
     6061 atgcagattc gcaaccagct cgcaagcgtg cttcgctgta tctgttcgca gcgcattatg
     6121 ccgcgcattg acggggaagg cagaatcgtt gccgcggaga tccttatcgg caaccgtacc
     6181 gtccgggaat tcatccagca atgcaggccc tttaaagaaa ttgtaaagct gattgaagac
     6241 ggtgcaagcc agtatggcat gcagactttt gatcagtcac tctattctct gtggaaggag
     6301 aaaataatca gcaaagagac cgctttgctg aatgcaacca gcagcaaaga cctgcaattg
     6361 cgcatgcagg gtctttattc ctgatcttca gaaagcctgc ccgcagcacg cccgcctgag
     6421 ccacctgact gcagtgtgaa ttttcgcacg tcacatggat taaaaaccgg catttgtgtt
     6481 attcttttgc catccccatg ctttcaggaa aggattgtta tggaacacgt aaccgagcag
     6541 gacattgatc actatgcgtc gttatcacag tttcagctga atgacgacac cagaaaacgc
     6601 ttcaccaaag aaatcaacca aattctggat tttctccaga ttttacaaaa agtggacact
     6661 gaatcaatag cgcccaccag acacggtaca gaccgtgtca atgttttccg ggatgatgtc
     6721 atccaagatc ctttgccaca tgccgaagtg ttcaaaaatt cccccctgga tgacggtgtc
     6781 ttttttcttg tcccgcgaat tatcgaagga gaagacaatt catgaaaacg ccatggtatg
     6841 aaaagacggc aaaagaaatt agggacgcag tacatcaggg tgaagcctcg gcactggata
     6901 ttgtcaattc ctttatagag catgcggatg ctgtggacgg cacagttggc gcgtatacgc
     6961 aacgttggtc tgaaaccgct cggacagccg cacagcggat tgatgagaaa atcagaaaag
     7021 gactgccagc aggcgctctt gccggggttc ctgttgctct gaaagaatcc atttgcacga
     7081 gcgaaggcaa aactaccggc gcctccaaaa tgcttgccga ctaccgcagc cccttcgatg
     7141 ctaccgcagt cacacggttg cagcaggccg acgcagtatt cctcggcaaa gtaaacatgg
     7201 atgagctcgg tttaggctca tcaacagaaa ccagtgcttt acagcgaacc accaacccct
     7261 gggctccgga cagagtaccg ggaggttcaa gcggcggcag tgcagcggca gttgcctcag
     7321 gcacctgtgc tctggctttg ggcacagata caggaagttc catccgccag cctgccagtt
     7381 tctgcggctg catcggtttc aagcctacct acggaaggat ttccagactg ggcattatcg
     7441 gcatcgcctc ctcactggat cacgtcggtc cgcttgccag aacggtcgaa gatacagctc
     7501 tggcactgaa tgttctttgc ggggaagata aaggagatcc cacctcctca gaccgtccgg
     7561 tcccggactt tactcgctct ctgggcagag acatccgagg gctcaggatt ggtgtgccta
     7621 aagaatatct tgcatcggat ctggacgctg aagtgcgcca gtatgtggaa aaagcattgg
     7681 atcaattccg gaacgcaggc gcggagattg tctccatttc acttccgtgc accggctatg
     7741 atgccgccgc ctatacggtt attagcactg ccgaaatgag tgccggcctt gcgaggctgg
     7801 acggtgtacg ttacggtgcc cgacatccgg aggcaaaatc agtaccggat gtatatgtta
     7861 tgagcaaaag tgccggtctg ggcacagaag tccagcgccg aattattctg ggcacctact
     7921 ttctttcggg aaagaatcgg caggattact tcgtcaaagc acaacaagtc cgtacggtcg
     7981 ttaaaaagga ttttcaaaat gcttttgagc agtgcgatat aatcgcaggt gcgacaacag
     8041 cgaccgccgc attccccttc ggaagcaagt ctgacaatcc tctggaaatg tacgcctgtg
     8101 atcagtttac cgttgcggcg aatctagcct cacaccccgc cctatccata ccctgcggaa
     8161 aaacagccgc agggcttcca atcggtcttc atctgcaggc acgttcctac gatgaagaaa
     8221 ctctactggg cgctgctttt tattatgagc agcatcgtgg ctttgaaatg ggtttcccct
     8281 ctattctctg aggaatataa caatgcaata tgaagcagtc atcggcatgg aagtccatgt
     8341 tgaaattaat tgtcattcaa aagtgttttg cagctgccct agtgcctttg atgctgcgcc
     8401 caattcccag gtctgcccgg gttgtcttgg catgcccggt gcccttcctg tgcttaaccg
     8461 caagatggtg gataaatcca ttgctgtggg tctcgctttg aactgccaga tcgcacgcag
     8521 cacccggatg gacaggaaaa attattttta tcctgatctt gccagaaact accagatcag
     8581 tcaggacgat cagcctttat gttatgcagg ctatattgac gttgacgtgg aaggccggaa
     8641 aaaacgaatc cgcataacca gggcgcatgt ggaagaggat accgcaagaa acagccacac
     8701 tcaggcaggc gggcaaagcg gcattgactt taaccgcagc gggctctctc tgctggaaat
     8761 cgtaacagag cctgatatca gaagtgctca ggaagccttc gcctacctca cgtccctgaa
     8821 gcaactgctc cagtacttgg acgtaagcga ctgcaatatg gaagagggat ccttgcgcgc
     8881 cgaagcaaat atcagtgttc gccccgttgg cgcagaatac tacggaacaa aaacagaagt
     8941 gaaaaacgtt gcctctttct ccggcaccca gaaagccatc gaatacgaag ttgagaggca
     9001 gatcgaagtg ctgaactctg gcggtaaagt ggtgcaggaa acgcggggct gggatgctga
     9061 taaaggcatc accgtttcac agcgcctcaa ggaatcagcc catgagtacc gctatttccc
     9121 tgaacccgat ataccgccta tcgtagtgga tgaagcctgg atcgagcaaa tcaggaaaga
     9181 acttccggaa ttcccgatag cacgaaagga acgctttcaa agccagtacg gtttaagcct
     9241 ctatgacgca caagtcctga cttttagccg tgccatggca gatttttatg aagcgattgt
     9301 gtccgccgga gccgatccga agaaagcagc aaactggctt atggtagaat tgcaggcatt
     9361 gctttcggat gcgaaactgg acatcagcgc cagcccggtc agtccggctt ctctggcgga
     9421 acttctcgcc ctgatcgaaa caggcattat caacggcaag atcgcgaaag aagttctggt
     9481 tgagatgttt aaatcaggcg cagcgcccaa aaagatcgtt gaggaaaaag ggctcggtca
     9541 aataagcgac agtaaagcgc ttgaagaaac aatacgcgcc attatagaag caaatcccgg
     9601 gcaggccgct gattatcgcg cgggaaaaga aaaacttttt gactatttcg tgggtcaagt
     9661 aatgaaagcc acgcgaggta aaggaaatcc tcaactggta aatgaaatac ttagacgtga
     9721 acttgattct taagtgcccc gtcaatagaa tacggtgaaa tgccaaatca acttaacgaa
     9781 aaagacaaat tggtgaaaaa agttcagcag gcagcggaag ggttgctgtc taaaaaagca
     9841 ctggacatca aagcgcatct tgtatccggg gcgacattga ttgcggatgc tttcgtcttt
     9901 tgctcggcga acagtgctgt gcatctgaaa gcagttgcca atggtgcccg tgaatggatg
     9961 cgcgacggag gctatgagat tctccgggta gaaggagatc atcagagcgg atggctgatt
    10021 ctggactacg gcgatgtgat tattcatatt ttcagggcgg aagcacgtgc gttttatgaa
    10081 cttgacaagc tatgggcgga cactcccgaa atcccgctgg ctcttgacat aaactgataa
    10141 ggagaggctg tcacatggac agtcagtaca cgcctgatat tcaacagcta taccacttta
    10201 aactgcagag gattctcggc gaaggcggta caggcagagt ttatcttggc gtagacatga
    10261 aaaaaggcac acctgttgcc cttaaactct tccgggaaaa ctttttcaga aaccgcctgc
    10321 acgtgcgcga tcttgcaaaa agcgtcacca aattcaagcg gttcaaaaac aataacgtag
    10381 tccagattta tgatttttac gacggcgatg aaggtcgctg tatgatcatg gaatacgtgg
    10441 acgggccgag catgaaatgg tatatcctga atcggcctta caatctggcg gaacgggtga
    10501 atgtagcgat ccagatttgc aggggcatgc agtacctgca tgataaaggg tgtgtccacc
    10561 acgacttcaa gccagccaac gtccttttta cccggaccgg cgttgttaaa atcgccgact
    10621 tttccctcta cggaagcagt tttcttctgg agctcatcga taaaaacatt ggcgaacaga
    10681 ttacgcccat gtttgtggcg cctgagttta tccgaaagga aaaagtaacg gcgcaaagtg
    10741 acatctattc catgggcatt tctttttata tgcttttcac ctcaaaggtc ccttttcccg
    10801 tggataatat ccgcaaactt tatcagtgtc acctggcagt aatgccagac cacccttcac
    10861 ttgccgctcc tgaatgtccc aatgcaatcg gtgacattat catgaaaatg cttgcgaaaa
    10921 gaccggaagc ccgtttttca gactgcgacg agttggcggt ggcacttgca cagacgcagc
    10981 gcagtcgcat ctgatttttc attttactga tctgtgttgc tggatagcgt tgcgtttatc
    11041 ttctcttttc attacaatgc aaaaagcgac ggtgaaaaac accacctgtt ttccgttttt
    11101 atagtagtat tttgcaaaaa aaattgaaag gctggcatga aaagaaaact tcgcatagga
    11161 attatcggct gtggcgctat tgctcaggaa ttgcatattc ccgaatatct ggatagcgga
    11221 gaagcggaga tcattgcgct cagcgatact gatctgaaaa aagcacaggc agcgaatgag
    11281 cgatattctc tgaatgccca acttttcccc tcatataaag acatgctcaa aaaagccgcc
    11341 cctgatgctg tgtccgtatg cctgcctaat tacctgcatt gcgccgctac ctgtgatgca
    11401 ctgcaggcag gctgccatgt acttgttgaa aagcccatgg ctgccagcat ggcagaagca
    11461 cgtaaaatgg tcgcttttgc agacaaatat aaaaagctgc tggcagttaa tcagagccag
    11521 cggctctacc ctgcgcatat caaggcgaaa gaagtgctgg acagcggcat tcttggaaaa
    11581 gtgctgcacg taaccgccat gttcggtcat gcagggccgg aacattggag cccatcggga
    11641 aaatggttct tcaacaaaaa acaggcgcgc ttcggtgcga tggcagatct gggtgttcat
    11701 aaagccgatc ttatccgctt tctcactggc aaagaagtgg cggaagtgaa tgccttctat
    11761 gaatgtctgg agaaaaaagg cgctacagta gaagacaatt ttgtttccac tttaaagttt
    11821 actgatggaa ccatcggtac gctttccgct tcctggactg tcaaaggcat cgatgccaac
    11881 tacacaatcc tgcattgcac caatggcacc ctgcgcgtct gcctgcttcc cgaccggccg
    11941 cttgttgcca atcttgtcaa tcccgaatgt gagattgtgt ttaaggttcc cgaacccccg
    12001 tccaatacga atcttggctg gggactcgac acaggcggtc atttctgccg agccattaaa
    12061 ggcgacgaac ctcttttctg cccgggcgtg gaagggatga aatcccttga aatcatcttt
    12121 gcagcggaag aagcggcaaa gaaaggacgc acagtcaaac tgacccataa atgagatcgg
    12181 ttccatggca caaacaataa ctgtctcttt aggcgaacgc agctatccga tccatatcgg
    12241 agaaaaccta ttgcacctca ttcccgaaat gctgcaaaag tccggtattc gcggaagcat
    12301 aggtgtaatt acggataccc atgtggctga gctgtacttg cagcaaacca tctcgttttt
    12361 gaaagaagcc gcttttcctt gcgtcacgca cattcttccg gcgggtgaag aaagcaaaca
    12421 actgtcacgc atagaagaga tatgcggcac ttttctggag gggcgtctgg acagggcaag
    12481 tgctgttgta gcactgggcg gcggagtggt aggcgatatc gccggctttg ccgctgcatg
    12541 ttttatgcgc ggcatccctt ttatccaggt tccgactacc atcgtggcgc aggtagattc
    12601 aagtgtaggt ggcaagacag cagtcaatca cgcattaggg aaaaacacga tcggatgctt
    12661 tcaccagccc tgtggcgttg tcatagacat gaccgtttta aaaacactcc cgccccggga
    12721 attgcgtgca ggctgtgctg agattattaa acacggagtc attctggatg ccggactttt
    12781 taactatatg gaaaaagaag cggcatctgt tctagcctgc gatttaaagg ctttgtttta
    12841 ccccgttgcc cgatcctgcg aaataaaatc tgctgttgtg gcagaagacg aaaaggagca
    12901 cgggattcgc gctctgttga atttcgggca taccttcggt cacgcccttg aaaccgtcag
    12961 caattattcc acctttcttc acggcgaggc ggtagccctg ggcatgtgcg ctgctgcgga
    13021 gcttggcaga cttctgggca ctgccagtcc tgaattttgc tgcagactgc gtgcctgctg
    13081 ccaggcgtac gggcttccgg tagtctggtc tgaactgccc gttagagaag cattgcaggc
    13141 aatgaaaaaa gacaagaaag cccgtgcagg cactttgaag tttattgttc cctgtgattt
    13201 cggcacggta gaacagcgga cggatatcac cgaagagctt gccgggaagg cgctggagtc
    13261 cctgctcaga gaaagctaaa ataacgattc aggaaaccct actatgttgt ttggcggaga
    13321 aactgcagaa agttattatg aagagggatt gacttcctct attaaagggg actacgaaca
    13381 ggcgcttgtc ttttttcaca aggcgctttc gttaaatccc gtttttgcag cagcacatca
    13441 ccaaatagcc aaaacacata tccgtctggg cgcctttcaa aaagccatag acgcactgaa
    13501 tcaggcggtt gccctgatgg gcagaaaaac ttccgctctt gccgatcttg cccaggcata
    13561 ctatctgcta ggcaaggtta aagaggcacg caccattttt tcagaaattc ttcagattag
    13621 cgaaaaagat acacggggtg tgctggggct cgccttttgt gcctttgctg atgaacagtg
    13681 ggttacggcc attaatcttg ttcaacatgc ctcaacactg ggacggcttc atttcgatgc
    13741 acattttctg attgcccgtg cagcagctaa aacaggcgct gaagacatcg ccgggttcca
    13801 ttttcagcag gctctggagc aaatgaacca caccctggaa atcacggaag agcaagtcac
    13861 gggctacttt ctgcgtggcc ttgtgcacgc ctccatggga ggtctgcttg atgcgctgcg
    13921 tgatatggat agtgccttgc ggtacatcga tcctgataaa aattattttg cttacaacca
    13981 tttttttaat aaagtggaaa ttcttgtaca aaaaggcgta cttttaaaag acctgaaccg
    14041 gatcagagaa gcaaaagaaa caggagaaca aatactggca ctggcgccag ataaccagac
    14101 cgggatgatg ctcgcttctc tttcatgatc gcatttgaaa ggattgcctg tgagtgatgt
    14161 tagccttcaa ctggtgcgcg agtattttga actgaatttt ttttacatcc tgcctcactg
    14221 gggaatcgtc gccgggcaaa ccaaagaact ggaaacaggg tcatctcttc tctttgtgga
    14281 gcagcagggg aaaccgaatc gcgctgaacc tgaattttta ttgcgttccg gcgatatcgc
    14341 cgctctgcat cgcgctgccg tcgaagtgcg tgcctggcat ggcggcaaac tctactcttc
    14401 cgctattggc gccacttccc tcttttcccg ggttgcttcg cgccagaccc acgaccttgc
    14461 aaccgccctt tttaaatcgg acaactataa gattgttctg gtcgtttccg aactccccat
    14521 ttctgaacct gcgaaaaaag ccgctctgga aaaactgcat gcccgcggta ttcaccatgt
    14581 cattgagttt aggacaattc tgggcgacct gctcaggcag atttcggctc agggtaatta
    14641 tggaccttcg aaaacgctgc aaacgcttaa actgctcaag cggtacggct tcattcaccc
    14701 tgaagagctg aggcagctta atctgcctct ggattcgtaa gaggatgccg gatagccttt
    14761 aacaacatca atgaaaggtc ggagtatacc cttctgacac agttaaaagc gcattttgcg
    14821 cttcgctgtt cctcttgggt gccagcatca cggaaagagc gtattccaga tcgtccttat
    14881 ctgcccgacc atcggaatgc tggatgtttt ccagaagtgc ttccctttcc atcgggtcaa
    14941 gaagccccat ttgctcaagc tttgaaatgg tttccatcac ctcttcctga attttaaaag
    15001 attcaaagac attgagatgg cgccgggcgg gaaacttcag gtcaaaagac atcttaccct
    15061 gcatgcctga agaaacaaat tcaaaggcta catttatatc cttctcttta aagccatctt
    15121 tctgcagctc tgcacgcagc acgtcgccca cagacactgc gccgttagag gccgcaatgc
    15181 gctgcataat aatagtaacc agattttgaa cgtcgttttt cattgaatgc tccggaagga
    15241 taacctctgc aagtttcctg catattgtac ttttgtttcg ccatatgaat caaaagagag
    15301 ccttccagcg cactataagg cttcttccat gctatacttc tactattcgt ctttctgtta
    15361 tcaacgatga ttgagtttgc agcacttcag gtcttttcta tggcaagcta tacactttcc
    15421 gccttccatg agcctcttat taaagtgcgc tttcaggagc gccccaaccg ctttcttgtg
    15481 cgcggaatcg atgcccaaaa acaggaatac ctcgcttttc tgcctaatcc ggggcggctt
    15541 tccgaactgc ttctgccgga tacatgcatt taccttatta aaaaatctcc ttctcaaaaa
    15601 gaacgggcga ctgacttcac cgcaatcgct gtcgagcggg acggcgttcc tgttatgctg
    15661 cacacacacc tttgcaacga tatggtggaa gtacttctga aaaaacaaaa agttcctgga
    15721 ttggaaaagg cttctatagt ccgccgtgaa gtgaagttcg ggaaaagtcg ttttgatttc
    15781 ctgctgcagg accaggcagg cgaaatttat cttgaagtta agtcctgtac ccttttcgga
    15841 agcggaatcg ccatgttccc tgatgcagtc actgagcggg gtacaaggca tttaatggag
    15901 cttgcacacc tagccgagac ctcacagttc cgatgctgcg tcctttttgt cgtgcaaaca
    15961 gatactgcac gttattttat gccggattat cacacggacc ctgtttttgc cgggaccatg
    16021 gttcaaacca aagatctgct ccggtatgtg cctttgcctg tagcctggaa cagcgacatg
    16081 tctttcacag ccgggcgcaa gcctttgcaa attccatggt catacattca gaaggagaat
    16141 gaagatcggg gtgcctattt actggttctg aaactggaaa aggacagtgc tgtgactatt
    16201 ggagcactgg gaacctttac atttaaagcc ggttattatc tgtatgtcgg cagcgctctg
    16261 ggaaatttat cagcccgtat cgcccggcat aaaaggccgc tgaagcgctt tcactggcat
    16321 attgattact tgcgtcaggt tgcaaaaaac gttgacgctt tccccatacg aagttccagc
    16381 aatatcgagt gcgctctggc tgacgcttta cgtcctttat acgagacgcc tcttccccgt
    16441 tttggaagtt cggactgcac ctgcaggagc cacctgttcc gatcttcttc ctatccggtg
    16501 catgatgctg agtttcacac tgttctgcag cgctttcgta tgcagaagcc accggagaac
    16561 actttgatct gaactgcttt ttctgtttca atataagcac tgcaagccaa atttccggaa
    16621 tcaccaatga actcttttac acaccaaaag ccttttaccc gaccacagat catcgctcac
    16681 cggggcgcaa gcgcttatgc accggaaaac acacggtcgg cttttctgaa agccattgag
    16741 ctgggtgcgg actggtttga actggatgtt caactgaccc gagacggcgc catggctgtc
    16801 tttcatgaca gagatgtcag ccgtttcgct gtgaaacata ttcctatata tgatctgacc
    16861 atgagcgacc ttcgctctct ggacgtcggc tcctggtttt ctgaagaata cgcccatgaa
    16921 cgtgttataa cgttggaaga agcactggag cttgcttcag gaaaaatcgg cgtttatatt
    16981 gaattgaaga gcactgttga tgaaacgcca cgcatccccg atatgcttga ggtcgttttc
    17041 cagtctaaat ccctgtctgc gtgggattgg agatacttgt ttgatgccgg gcatcgcatc
    17101 agtgcagaca gcatggttct ggctcagcgt gccattgatt tggtcaggaa atacagagat
    17161 caatgccgca tggtcgttca ggctttttca ccggtgattg ccatggtgtt ccgcaaagag
    17221 gccccggata ttccttttga atttttgggt atggatcttc cggaaccgcc caacatctgg
    17281 agagacttcg tgctgtttgg cgaaaagatt gatgtggaag gttttaacgt ttgccgaact
    17341 tctttaacag aagaacgctt cctttatttt cagaaaaaaa agaaacgctg cgctgtatgg
    17401 gtcgtagatg aaccggaaga gatccggcag tttaccgcga tgggcgttca tggactcatt
    17461 tcaaataaac cggatatctg ccgtacggaa ttatcggcat tggggtactg aaaaaccagg
    17521 ggaatcaatc tttttctcca caactgccat gcctgacagc gagaagaaag ggtgtcaatt
    17581 tggaaggtgg ctttgcattt gtcgactgga caatcctttt tatttatatg ggtgttatgc
    17641 tgctgattgg cgcactggtc tcccgccagc agtcggacag ccagtttttt ttcctgggcg
    17701 gtcgctccat gccttcctgg gcagtagccc tatccgttgt tgcaaccgct ttgagcgccg
    17761 ctacatttat cggcgtgcct cagatggctt tcgaaggtga ttttacctat cttgcaacca
    17821 atctgggcgc tatgatagcc gcatttatta ttgcgtttta ttttgtaccc cctatttatg
    17881 ctgccggaac cgttaccatt tacggatatc tcgggcagcg gtacggagag agcgcacgaa
    17941 cggcagccgg cgccatgttc ctgctgggca ggctcctctc ctccggcgca cgacttttta
    18001 tggcaggcat cggcttttca cttatgctct atggcgaaac agcccggcat catcttattt
    18061 ttgccatcct tttactcggc attgtcggca ctttgtacac cgtttcaggc ggcatccgtg
    18121 cggtaatctg gacagatgtc atacagataa ttgttatggt tgtagcagcg cttgccgctg
    18181 tagcctatct cgtatccctg atccccctga ctccgggtca aatggttgca gaactcacca
    18241 gctcccaggg cgtcaacaag ctgaaagtac tggatttcag cctgaatttt tccaaccctt
    18301 ttacgttctg gtctgctctc tttgccatga ccattgtaag cctttccacg cacggggttg
    18361 atcaggacat gctgcagcgg gtaatgaccg caaaatcacc ttttcatggc gggcttgcac
    18421 tgatcggttc catggcaatt gcagtgccta ttatccttgt tttccttatc ataggtgcgt
    18481 tgcttcacct tttttataca cggcctgatc tgatgggcaa tgcgttcccg cccgatgtgg
    18541 tggaagatac ccgcaggctc tttccccaat ttgtgttaca gcatcttccc gtcggtctga
    18601 gaggtctgac aatggcaggt ctgattgccg ccgccatgag caccttcgat tccgctatca
    18661 acgccatggc gagcagtctg attgccgact tgtatgtgcc tctccgggcg ttcctgcaaa
    18721 aaatgaagag cggtcaaaaa gaagaagagc accttcccga tcttgccgga tcacgatttg
    18781 ctgtagggct tatgggtttt ttactgactc tcttcgctat cggcgcgatt tatttgcagg
    18841 aacagggcgg agaatctctc attaactttg ccctgggtgt tatggctttt gcacaggctc
    18901 ccctgctggg tgtattctgc accgcactct ttacgagacg cggaaatgcc gcctctgcct
    18961 tgactgcctt cgccacaggc atagggcttg tgctgctgtt acagccttat atgctgccca
    19021 agtgggcagg ctttactttg ggctggacct ggacctgggc tatcgtcagt cctgtttgtt
    19081 ttctgatctg cctttgcgga agcccgggca cagaggaaat ttcatgaata attccctttt
    19141 tccagaagaa aagccggttc cctcctctct tcccaaaaaa gaagcaccgc tcgcccgccg
    19201 tgtcgcccca aagtccctcg atgaaattct tggacaaagc cacattctgg gtgaaggtaa
    19261 actgctgcgc cgcgccattg aagcagatcg catcacttcc attattttgt atggcccgcc
    19321 cggatgcgga aaaacagcac tggcgcgcgt tatcgccatg cggaccaaag cggttgtgga
    19381 accactcaat gctgttcttg ccggcgttac cgatatccgg cgggtagtta agtctgctca
    19441 ggaacgtttt acaacggcac agcgtgctac aatccttttt cttgatgaga ttcatcgctt
    19501 ttcaaaaacc cagcaggacg ctctgcttcc tcatgtggaa aacggacttt ttacgcttat
    19561 cggagcgacc actgaaaacc cctatttttc aatagtctct cctcttttgt cgcgttcgca
    19621 gatcttcgaa ttcaaagcac tctccgaaga agacattcag gtacttttaa accgcgccct
    19681 gacgcatcgc ggtcgcggct tttccggaat agaaattcag attgaaccgg aagcggcagc
    19741 ctacattgct aaattttcag aaggagacgg ccggcgcgct atcaatgcgt tggaattggc
    19801 aattctgacc acgaaacctg tagaaaacac catctgcatc accctgccca ttgcggcaga
    19861 gtccatccag cagaaactgc tccattacga tggaagcggg gattctcatt atgatgcggc
    19921 atccgccttc atcaaaagta tgcgcgggag tcatccggat gccgcgttat tctggatggc
    19981 acgcatgata gaggccggtg aaacaccccg ctttatagca cgccggatat gcatcgctgc
    20041 agccgaagaa gttggctgtg ccgatccgat ggcgctggta cttgccaacg ccgcttttca
    20101 ggctgccgag tttgtcggtt ttcctgaagc gcggatcatt ctggctgagg cggctgtcta
    20161 tgtggcatca gcacctaaaa gcaatgcctc atatcttgcc attgatgcgg cgctcgggat
    20221 ggtgcgtgaa gggaaaagca ttgctgtccc ggatcacctg aaagacagct cctaccaggg
    20281 tgcgaagaaa ttgtcgagag gcatcggtta tcgttatgcc cacgattttg aagatgccta
    20341 cgttgctcag gactatggcg ttccccgcag tattttttat aagccaactg agcgtggtca
    20401 ggaagaactg ataaaaaaga gattggatac tctggaagaa atagaccgga acgctgctga
    20461 gtctgttcag ccttcagaag agccgcaata agccagacgg catgaccgta tgtcaggtat
    20521 cggacaccga atccatctgt tttttgattt tgtcgaggag cgcaggactt ccggtcaaaa
    20581 ccttgttaaa tttaccgagg agatcactcg tttcaacagt gatcagatgc tccattttac
    20641 aggcttcctc agttgccagc tctgaactca gcccgagcac cgactcaaaa aaacttcgca
    20701 atacttcata attacgggaa acaaggcggg tcatcttttt gccttctgct gttaaaagca
    20761 gaaaacgatt gggatcttca gtaacccatc cccgcttttt taattgtgaa agcgccatgg
    20821 aagccgcacc gcgggatact tccagcattt cagcaacatc agtcgtccgt gcgtaaccca
    20881 gaccgtcacg gagagattcg atggcgagca gataatgtgc ccgcgaatgc gacagattgc
    20941 tgtaggtgta ttcgcgccag ctcgcccctt ccgcactcat atcacgttct ttagtcccat
    21001 cctgggattg tgctttatca ttcaaaatat cctgagtctc cataggtagt ggacttcagc
    21061 agataatcag gaactgaagg ttttgtaatc tttgccttta cagcctgatt aagtaacacc
    21121 agctctgatg ttaagtattc catacaacta ttcagcatct caaccagcaa acacctctgc
    21181 agataacagt atcacagagc ttaaccggtt atgtcaagaa gtttctgtaa attggcggat
    21241 tcaaggctga aatgcaatta ttgaattgaa taagacttcg gcagg
//
LOCUS       DAOPWB010000047        21246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2474_length_21246_cov_5.571327, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000047 DAOPWB010000000
VERSION     DAOPWB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 21246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21246
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2474_length_21246_cov_5.571327"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="PKN92_06140"
     CDS             complement(<1..161)
                     /locus_tag="PKN92_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ48409.1"
                     /translation="MRILVTGAGGHVGGAVLQRLYAEGHSLIALGRNPLPSLSPEIEM
                     LQVDITSPDA"
     gene            complement(161..1237)
                     /locus_tag="PKN92_06145"
     CDS             complement(161..1237)
                     /locus_tag="PKN92_06145"
                     /inference="COORDINATES: protein motif:HMM:NF009402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNZ48410.1"
                     /translation="MTSSHDSSARLTLLVLAVGGNVSQGILKALKRSSLSCRIIGTDL
                     SELQMGLYTVDRGVLAPHASEPGFEEWLVDLCLKEDVDAVLSGCEPVLRVLAEIRERV
                     ESRTKAKCLVCSPEIWRRFDDKLLGCAWLKEQGFDYPDFAAMEDDAEVERLVSGHGYP
                     LLAKPRIGGGSTGIVRVGDDADLAYVRKREGYLLQEYLTGKEKEFTIGCFSDRDGYLR
                     GSIVFHRTLLAGTSYRITAGAFPEVRSMAEAIVRRTGITGPCNLQFRLTERGPVCFEV
                     NPRFSGTTPIRAAFGFNEVEAALRHFVLGEEGIDLPDVTEGIALRYWNELYVPVEARD
                     QLLAEGSIDPSVFPGIITEPYGGI"
     gene            1624..2325
                     /locus_tag="PKN92_06150"
     CDS             1624..2325
                     /locus_tag="PKN92_06150"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ48411.1"
                     /translation="MKQTSIVLVLLFLGLSLFAQADDWSEKNFVTMPPDVMFLLMELT
                     NAKPLFKTDLSNAICEKDGWEFSNGILTARGKGDIWTEEEYGNFMLALEFRCEADTNS
                     GVFLRCDDLDDWINSAIEVQILQDNAGAKNTRHRCGGIFDCVTPRDAKLKDIGEWNEY
                     VIIVKDNHIYVLLNGEQVTGVNLDLWTEAGKNPDGSPNKFKKAYKDMSRRGHIGLQYH
                     GQAISFKNMRVLPLD"
     gene            2358..2765
                     /locus_tag="PKN92_06155"
     CDS             2358..2765
                     /locus_tag="PKN92_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNZ48412.1"
                     /translation="MIPATKSIYLCGPIMDASPDDSKSWRQRAKERLAGRFILLDPMR
                     RNFRDREIDSANEIVEFDLQDVRTADILLVNYTKASIGTSMEVFYASHDLGKFVIAFC
                     PENYQDMSPWMVKFCTKILPDLNGALDYIERHF"
     gene            2844..3347
                     /gene="purE"
                     /locus_tag="PKN92_06160"
     CDS             2844..3347
                     /gene="purE"
                     /locus_tag="PKN92_06160"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNZ48413.1"
                     /translation="MEHSCEKALIAIIMGSESDWETMHRAHTIMKDFGVPHCCRVLSA
                     HRTPDLLAERVRTADANGVEVFIAGAGLSAALPGAIAALTTKPVIGVPIQTGAYNGQD
                     ALLAMVQMPPGVPVGATTIGPAGATNAALLAISILANSHPEYREKLQQYREEQVARVA
                     AIKLPCE"
     gene            3397..4674
                     /locus_tag="PKN92_06165"
     CDS             3397..4674
                     /locus_tag="PKN92_06165"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HNZ48414.1"
                     /translation="MFSAPTDKQITQGYAHAREVYGALGVDVDAAMDRLASIPVSLHC
                     WQGDDVGGFESLSSASGGGIQATGNYPGKAVTPEQLRQDAAKALSLIPGKHRFNIHAM
                     YAETGGVKVERDALEPAHFQNWIDWAKAQGVGVDFNPTCFAHPKADDGFTLSSYDAGI
                     RQFWIDHCIASRKIGAAFGKALNNPCVTNLWIPDGYKDVPVDRNRSRELLRDALDAVF
                     AEAIDPSENLDAVESKLFGIGSESCVIGSHEFYMGYALSRKKLLCLDAGHFHPTEVIS
                     DKISSVLAFLDEILLHVSRGVRWDSDHVIMLTDEIRAIAEEIIRNNFESRVHIGLDFF
                     DASINRLAAWVIGTRVMVKALLIALLEPTNHLRELEKSGNYTARLALLEEVKTLPFGA
                     VWNYYCVKQNVPPAGAWMLDIQRYEKEVLALRG"
     gene            4750..5889
                     /locus_tag="PKN92_06170"
     CDS             4750..5889
                     /locus_tag="PKN92_06170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNZ48415.1"
                     /translation="MSASAIPTATLKAREERRIQRGHLWCYRNEFMSLPDLEDGDVFD
                     VYSSGRRFVARAFYQAEGGICGRVLSYHQTEINRSFWKKCLQDALVLREHLFPRSVVY
                     RWIHAESDGLPGLVADRYDSVVVLQSGCAFYRRHWQSLQEILLAVPGVSSLTFAFREE
                     RLEAGAAPEKQVLELNGLRLGFLPAGAQKTGLFLDQRENWQLAERFAPGARVLDGCCY
                     HGLWGLHAARGGALSVTGVDSSAAALAQAEENAGMNAFEDRFTFIKEKVEKHLQECTD
                     YDLIVLDPPAFAKSRKQQNAAFALYENLNSSAMQKLTPGGILISCSCSHFLDGALFDE
                     MLKRAARSTARQVQLLMMRGASPDHPVLTAMPETSYLKCAVLRVL"
     gene            complement(5908..8262)
                     /locus_tag="PKN92_06175"
     CDS             complement(5908..8262)
                     /locus_tag="PKN92_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNZ48416.1"
                     /translation="MNRSARFLFFLCLFLTGIMAAGAPASAPDKKNTAALETATQENA
                     VSADSESAKNQELILNLETRASEMEIQIQEWAEQESASAVGITEFSFTALQGRLSTLR
                     KLQSAWQRHVNALKEYEALQERQALFDEEVKSFTRLTEDPPYSMAFLDGLRDQAALKQ
                     MDIDAEKLSLRAYNELAGMEQADIAQAKAALNRVAEQLRLAPQEERAALQFESETAKL
                     QVQASEAEYQAAAMELKRKERQLQLLEGELAFARKKVEMARVQTLFRQEELDEIIKRE
                     QGLIESLQSEAAKARDSIDQHRNRMLTAERALARATDPEAQQQAKNDLDHLRRQIDAG
                     RARIAGIEALLEYEDYVQKLWQLRFRIANPALAKVRVDLGEVVQIFHERLAVLDKERD
                     AGERRSISLRSRINTLEQQLAEWKEEQGSKKPTERELESLQENLRLRNRMQLRLVEVI
                     TLANRIVEEARILQKERSWVQVGKDWLFTAKETTLLAFDHELGVIGEESITGRKLFYM
                     LAILVVGLVVSRMVTRIIRNYAVNRLKLRSNVVMIVAKLTNYILFLTVVYFALNYVNI
                     PLTIFAFLGGAVALGVGFGAQNLINNFLSGLILMGEQPIRLGDIIEIDGKTGTVTDIG
                     GRSSRLRMFNGYDVIIPNSKVLETSVINWTLTDLKYRVQVDVGVAYDSPTRDVAKLLL
                     YAVSDHGQVLTDPPPKVLLSAFGAHALEFTVYFWLERSANVDGTVITSDIRHRIVRLF
                     REAGIEIAYPQQDVHLKSDTPLQVTFSDNTRNADDNEEAQGRLP"
     gene            8261..8419
                     /locus_tag="PKN92_06180"
     CDS             8261..8419
                     /locus_tag="PKN92_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48417.1"
                     /translation="MVCLPADYIKEIKNGCEKEGDAVGLHYRNPGRQSQPEDAYATGM
                     TADPVMPA"
     gene            complement(8464..10599)
                     /locus_tag="PKN92_06185"
     CDS             complement(8464..10599)
                     /locus_tag="PKN92_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNZ48418.1"
                     /translation="MRTILTLLCCCVLFLSASAFAEREVKRFYVSTDGRDTWSGELES
                     PADSGEDGAFATIAGARDAVRKWRSATEGNEKIPVEVLIRDGEYPITEAIVFTPEDSG
                     FKEAPVVYRAYPGEVPVLFGGRRLTSWELREDRWVASVPEARGSDFSVGAFWVNGDRL
                     DSARFPKAAHTAGDYPAASDFFYTDGPVFKKAADKNEEVKSSTELRFHEGDIRPWEHL
                     DEALFIVFHSWATSMHRVKKADFESRILEFTGAARWPFAQWTDRQWYFVEHLMEALTL
                     PGEWCLDRKAGELHYLPRTGETPETVDAVIPLTRQFLILQGDPAEEKFVSYLHFNGLH
                     MYYGDWPIGAAGHSDAQAEISVEAAVQAKGARHCLFESCEVSHVGSYGLWLREGCKDN
                     RVERCAFTDLGAGGVRIGEAFDAKLVPCLTEYNTVDNCLLHDGGRIYRSAVGVWIGRS
                     SHNTISRNEICDFRYSGVSVGWSWGYDPTSAHHNKITGNHIHNLGKGQLSDMGGIYTL
                     GVSPGTELRNNYIHDILSNPDISGGWGLYTDEGSTHILLTDNIVCNTTTSGFHQHYGR
                     ENKVVNNIFAFARKEQVFRSRDETHISFFFENNIVYFDQGELLSGAWSKGNFVINRNC
                     YWDASGREIQIKGQSFENWQRSGEDKDSIIADPLFVDIKGRNFTLKEDSPALKLGFKP
                     IDLSQTGLYGDDAWVARAASLKRITEETK"
     gene            10823..10901
                     /locus_tag="PKN92_06190"
     tRNA            10823..10901
                     /locus_tag="PKN92_06190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10859..10861,aa:Pro,seq:cgg)
     gene            11314..11976
                     /locus_tag="PKN92_06195"
     CDS             11314..11976
                     /locus_tag="PKN92_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48419.1"
                     /translation="MVLLEEVTRRLGPGVHFSCEKFYAAPGSRTALWGPSGCGKSTML
                     NLITGLLRPDSGTLRIDGADITALSESRMDAFRGERMGFVFQTFNLLPPFNALQNVLL
                     GMRFSDTIPEQEWKSRAVAMLNRVGLERRLHSKPGTLSVGERQRVAIARALVNKPRLI
                     VADEPTGSLDPKTAASVMALLLELCEEEKVTLLLVTHDRGIAEQLPDLFDCTGLARED
                     AS"
     gene            11973..13259
                     /locus_tag="PKN92_06200"
     CDS             11973..13259
                     /locus_tag="PKN92_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ48420.1"
                     /translation="MSLWSIAWRYLWNRKLTTALTILSVALGVGMISAVLTLREETQR
                     RFEEEGQAFDIVVGSKGNPLQLVLSTVYFMDAPTGNMDYAVFESLKSHEDVTHAFPIA
                     MGDSYKGYRIVGTSRELMDFAPGARSPYTLAEGRYFEKAQEAVLGAQVARDSGLKVGS
                     VFVGTHGLVQSSFAESHDDHPYTVVGILNLSGSPNDRAIFCDIQSVWDVHSHCDADHD
                     HDSDSDHDHDHAEDPDHDHDHAHDHDHEGQEVTAALIKLASPALRFEFKDQINKHSNA
                     MAAIPIMEIQKLYEQLLGTAKLVLLAIGYLVVAISSLSILIGLYMAILQRRRDLAIMR
                     ALGATRGEIFGAVIIEAFWVTVLGIAAGWFMGALTCVVLNHFLVTRIGFHVPAIHMTA
                     DLVSAYSAVVLMGLIAGILPAWQAYRSDVARDLAEL"
     gene            13324..13998
                     /locus_tag="PKN92_06205"
     CDS             13324..13998
                     /locus_tag="PKN92_06205"
                     /inference="COORDINATES: protein motif:HMM:NF023164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HNZ48421.1"
                     /translation="MRKRTKRDLTTLIGLVVIVASVVGVNGYMRRAGLRETYEKMRAA
                     LEAHHRSEGVALIDWKELQAVVGKRKTGATFPASLKEKDGRLVNIVGFMNAIDQFRNV
                     QEFMLLPVPLTCYFCDAPPMRDIIEVKLDKPADMVNEPVLIGGRLRLHEGPGPLFFYT
                     IENAKWNEAVQDEEMTEKVTEQQHRLHLIEGFRELKGESALLNESAEEEMLPGYTPPQ
                     HTGTEE"
     gene            complement(14015..15475)
                     /locus_tag="PKN92_06210"
     CDS             complement(14015..15475)
                     /locus_tag="PKN92_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNZ48422.1"
                     /translation="MNQAQSKNMWLPMAGVLLVALLLLGLGLYLVLTGEHAAVSTERP
                     LPARKAADHAPDMVLIVLDALRADRVGAERNGVPLTPFLDRLRAEGAWFPDAVTACTW
                     TRPAMASLFTSSYMETHEVVFAAEDEASGNRQTALPPDRQVLAALLQAGGYATAGVQT
                     NGNLFPVMGFDRGFDHYTCSLDAPGSWVTQESLKAVPSLKSPFFLYAHYMDTHLPYQS
                     PEEYRKLLGYAPEKLTAEEQVAVEHFLEYVMAWAHFSTGRSETFPLQELSEDGKEAVR
                     LLYDASVRSVDEEVRRLVEGVRARSSDALVIVMADHGEHFWDHNLLGHGLSLYNCELQ
                     VPLILQGGGVPKGEQSRLVETIDIAPTLLRLAGLPAPESWQGKDLFTAEARHGFARTQ
                     SDSPAWNIEMDAVLTETLKLVVDERKQQVLLFDRKADPEEQQNLAEERPEAVAAMKQL
                     LHRQRAENQRRRTGVQSEVTVDAETLEQLRQLGYMH"
     gene            15729..17198
                     /locus_tag="PKN92_06215"
     CDS             15729..17198
                     /locus_tag="PKN92_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNZ48423.1"
                     /translation="MNRYFSGGTMSRRAWLAASGAGLAAAFSNSKLFAADGSPAPTEA
                     PLPNIVVFLSDALRADHLGCYGYPFPVSPRIDAFARESLVFEQCYSQAPWTKPSVSSL
                     YTGVLPVVHQSVVTSFEQESFWHWDETNPQGFEIQILRESFTTLAEALKARGYQTACF
                     QYTPHCQQGFGFARGFDHYRYVPVEPVVPQTDALIKWLQEEAKEPFFIYVHELDPHGP
                     YTPGEALYEKLFGKTLQRAREELSEFDRAIIQKFQMPDLLHLSREGLEHLISLYDAEI
                     LGVDEQFGRVLDCLQGKGVEGHTVVSLISDHGEAFGEHGFFKHNNSLYKEELHVPLII
                     RTGEGSQHRRVPWRVSLSDLYPSLLQLAGAAVPDYVQAAPLFDAQGALTVTESRPVYA
                     YFDHNRPDISSWEFCMTDGDLKIYDKGDGKPPEIYHQKEDPDEKRNIADALPQAAELQ
                     EKIKSARAKHEELSARFGPPQYAGIDMKSMEELRALGYF"
     gene            17256..18884
                     /locus_tag="PKN92_06220"
     CDS             17256..18884
                     /locus_tag="PKN92_06220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ48424.1"
                     /translation="MRMFRCLTDAGSSKQVCLLLLICFIAAALRLYGLAGEGLWYDEG
                     CSIYLSDYASRCDRLLNMDYCNDPPLFLVLLLGWRRILSSISGSLATDFMLRLLPCFF
                     SVITVPAVYGAFRGLLQYRTTPETSPQDSRFAELSALAAAFLVAVSPFHRYYAQELRC
                     YSLLTFLAVLVLWNFIAALRDNRLWQWITLTLLLSLSLWTHFFSAWLFLVLDLYLLLT
                     FRAHKKRYIPWALCHIAALLLSLPALLLAWRMSQVVAAIAVQWIPPPGLKTLFITFKT
                     FFAGYSPRSLVYWPLFLLSAGLCLFGVRALRHSRQNLLLLLLWAGLPMVSSAVLWRIR
                     DFSYYELRLFIWCAPAAALLAACGWASMRFRWRMVIGSIFLLLTGMLLSDSIAHALHP
                     VPQHRLGVRYKVDNRGAARYLAAYAFAGEPVYHSSHVTLPSFRFYLPGHEHTHICLTR
                     DDREGFFSAYPNRPLWTHLNFQPTPWHALASFPRSFWLVRSWWEPFEVPENLFLLEKE
                     IEQQCVREEELAFFGITLIHYQRRGTPSGQEFLQ"
     gene            18995..19957
                     /locus_tag="PKN92_06225"
     CDS             18995..19957
                     /locus_tag="PKN92_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48425.1"
                     /translation="MIDSFFHYITPAEKYKLVEEKAPVKYDQKAGFVINDDGTYKRSF
                     VFRDGSPPLDSTFTLQNGMRHTPVSGEKRDKFALFFGDSFTFGVGVNDTETLPYYFGE
                     EQPGYMPYNFGIPGGSVQHMYLKLQGKEIEAKVKEQDGIAFYWFFGFHTRRAVGGMPA
                     FNRWADMMPCFELEEDQLVYKGNFRDAHPRRALLYDLLYKSSTVRYLNLFLPRTFSEY
                     DYQVTVQLLLESYRLFKESCPNGKFILLMWDNRDSYTPVQKMISEAGIPVILIKEFFF
                     GVDNPLETGQTVPDGHLTGPGYQRIAKGLAAHSLLSASAAYEKK"
     gene            complement(19974..21077)
                     /locus_tag="PKN92_06230"
     CDS             complement(19974..21077)
                     /locus_tag="PKN92_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ48426.1"
                     /translation="MSGRFATLARGYLNRSTPGYMIFFVTPLCDCRCVMCFNREVIDH
                     AAHRSPLRIEEIERIARNFPGLHHVNFSGGEPFLRKDFAAIPELFYKHSGTRLFACPT
                     NSSHPDRIIPAVRSICKSCPDAWVRITQSIDGIGEDHDAIRGKAGIFECVKTLNHELA
                     LLRKELPNLSVGMAMVLSKFNQGKEYDILEYVHRTFRFDDFGALYVRGDTHDPEAAKI
                     NNEAYAAFVRACREKTEQKATGKSWTGKLFTAITRQASDLIMQSVVEDRFVTPCRAGK
                     NMIVMDDEGRISPCEVLEYFIKTGISSLDSAVMGSMRENDYDIRKILQSEKARTIVAH
                     IKESKCHCSFECAMSVNVLYSPSLWPGLIRHLF"
BASE COUNT         4770 a         5676 c         5626 g         5174 t
ORIGIN      
        1 gcatccggag aggtaatgtc gacctgaagc atttcaattt ccggggaaag ggagggcagc
       61 ggattcctgc cgagggcgat caggctatgc ccttccgcat aaagccgctg cagcacggca
      121 ccgcccacgt gtccgcccgc tccggtgaca agaatgcgca tcagattcct ccgtacggct
      181 cggtaatgat acccggaaag accgaagggt caatagagcc ttcggcgagc agctgatccc
      241 gcgcttcgac aggcacatag agctcgttcc agtaacggag cgcaattcct tcagtcacat
      301 cgggcagatc aattccttct tcgcccagca caaaatgacg gagcgccgct tccacttcat
      361 taaacccgaa agcggcacgg ataggggtcg ttcctgaaaa acggggattc acttcaaagc
      421 agacgggccc ccgttcggtc agccggaact gcagattgca cggccctgta atccctgtgc
      481 ggcgcacaat ggcctccgcc atgctccgaa cctccggaaa agcgcccgct gtgatccggt
      541 aactcgtccc tgccagcagc gtgcgatgaa atacaatact cccacgcaga tagccatccc
      601 gatcggaaaa gcagccgatt gtaaactctt tttccttccc tgtcaggtat tcctgcagta
      661 aatagccctc tcttttgcgc acataagcaa gatccgcatc gtcgccgacc cggacaattc
      721 ccgtgcttcc gccgccgata cggggctttg ccagcagcgg gtacccgtgc ccggaaacaa
      781 gccgttccac ctcggcatcg tcttccatgg ctgcaaaatc ggggtagtca aagccctgct
      841 ctttcagcca ggcacaaccc agtaacttgt catcaaagcg gcgccagatc tcgggcgaac
      901 agacaagaca ctttgctttt gtccggctct ccaccctttc acggatctcc gcaagcaccc
      961 gaagaacagg ctcgcagccg ctgaggacgg catccacgtc ttctttgagg cagagatcga
     1021 caagccattc ctcaaagccc ggctcagatg cgtgcggtgc cagaacgccc cggtccaccg
     1081 tatacaatcc catctgcagc tcgctcagat cagtaccgat tatacggcag gaaagactgc
     1141 tccgtttcaa tgcttttaaa ataccctggc tgacattacc acccactgcc agtaccagca
     1201 gagtcagtcg cgcggaggaa tcgtgagaag atgtcataaa aaactgctcc aaagaataat
     1261 gtatctactg tattctacaa caaatacaga tatctgcgag actttttcgc agagacggga
     1321 tgcagattca actttgaaac gtaaccagtt agtaccgtaa aacaaagaag aactgaaaaa
     1381 ggaggatgtt atgaaagagc gcattttgat ccgggagcga tccgatgata cattgattcg
     1441 tgcgccttga aaaagaataa actgtgaatc cggcatttta cccgacgatt aggtataata
     1501 cgaagtggca tatctaatat tggtatacta ttcagtgctt ccggaaatgg taggatccgg
     1561 aaggtttttt ccaaacagtg acgcggctgc gtcctgttta aaccacaata ctggagtgga
     1621 gtaatgaaac aaacatccat agtgcttgtg ctgctttttc tgggtctttc cctgttcgct
     1681 caggcggatg actggtccga aaaaaacttt gtcaccatgc ctccggacgt aatgttcctg
     1741 ctgatggaac tgaccaatgc gaagccgctt ttcaaaacag atctgtcaaa tgctatctgc
     1801 gagaaagacg gatgggagtt cagcaacggg attctcactg cccggggcaa gggcgatatc
     1861 tggactgaag aggaatacgg caattttatg ctggctttgg agttcagatg cgaggcggat
     1921 acaaacagcg gagtttttct gcgctgtgac gatctggacg actggattaa cagtgcgatt
     1981 gaagtacaga ttctgcagga caatgcaggc gcaaaaaata ccaggcaccg ctgcggcggc
     2041 atttttgact gtgtgactcc gcgggatgcg aagctgaaag atattggcga gtggaatgaa
     2101 tacgtcatta ttgtgaaaga caatcatatt tatgtcctgc tcaacgggga acaggtcacc
     2161 ggagtcaatc ttgatctttg gacggaagcg ggtaaaaatc cggacggatc ccccaataaa
     2221 ttcaaaaaag cctataaaga catgagccgg agaggacata taggtcttca gtatcacgga
     2281 caggctattt cttttaaaaa tatgcgtgtg ctgcctttgg actgaggtac agctcttcta
     2341 taaggacaga tgtttttatg attccagcga caaagtcaat ctatctttgc ggccctatca
     2401 tggatgcctc acccgacgac agcaaaagct ggcggcaacg cgccaaggaa aggcttgcgg
     2461 gaagatttat cctgctcgac ccgatgcggc gtaatttccg cgaccgtgag attgacagtg
     2521 ccaatgagat cgtggaattt gatcttcagg atgtgcggac ggcagatatc ctgcttgtaa
     2581 actatacgaa agcctccatc ggtacctcca tggaagtttt ttatgcatcc catgatcttg
     2641 gaaaattcgt catagccttc tgcccggaga attatcagga catgtctccg tggatggtca
     2701 agttctgtac taaaatcctg cccgacctca atggggcgct ggactatatc gaaagacatt
     2761 tttagtgtga tcctggtgta aataagggca gtctttccct gttgacaacg ggaaggcgca
     2821 aacgaaaaga aagggaaaga attatggagc attcctgtga aaaagcgttg attgccatca
     2881 tcatgggcag cgaatccgac tgggaaacca tgcaccgtgc ccatacgatc atgaaagact
     2941 ttggcgtacc tcattgctgc cgtgttctgt cggcacaccg tacccctgat cttctcgccg
     3001 aaagagtgcg caccgctgac gccaatggcg tggaggtttt tattgccggt gcggggctgt
     3061 ctgccgcgtt gccgggtgcc atcgccgcac tgacgacaaa acctgttatc ggcgtgccca
     3121 ttcagaccgg tgcctataac ggccaggatg cgctgctcgc catggtgcag atgcccccgg
     3181 gcgtcccggt aggtgccacg accatcggcc cggcgggtgc gaccaatgcg gcgctcctgg
     3241 cgatttcgat tcttgcgaac agccacccgg aataccggga gaaactgcag cagtaccggg
     3301 aagagcaggt ggcgcgggta gcagccatca aactgccctg cgaatgaacc gacagcgaca
     3361 gctgccggta aaaactaatc atagaaaggg atagccatgt tttcagcacc gacagataaa
     3421 cagattacac agggatatgc gcatgcccgg gaagtgtacg gggcacttgg agtggacgtg
     3481 gatgccgcca tggatcggct tgcgtccata cccgtttcac ttcattgctg gcagggagac
     3541 gatgtaggtg gttttgaaag cctcagttct gccagtggcg gcggaattca ggcaaccggc
     3601 aattatcccg gcaaggctgt tacgcctgag cagctccgtc aggatgctgc caaggcgctg
     3661 agtcttattc ccggaaagca tcgcttcaat atccatgcca tgtacgcgga gacaggcggg
     3721 gtgaaagtgg aacgtgatgc gctggagcct gcccactttc agaactggat agactgggcg
     3781 aaagcgcagg gcgtaggtgt tgatttcaat ccaacctgct ttgcccatcc caaggctgac
     3841 gacggattca cactgtcgag ctacgatgcc ggcatccgtc agttctggat cgatcactgc
     3901 attgcctcaa gaaaaatcgg cgcagccttc ggtaaagcac tgaataatcc ctgtgtcacc
     3961 aatttgtgga tccccgacgg ctacaaggat gtgcccgtcg accgcaaccg ctcaagagaa
     4021 ctgcttcggg acgcactgga cgccgttttt gcagaagcca ttgatccctc tgaaaatctg
     4081 gacgccgtgg aaagtaaatt gttcgggatc ggctctgaaa gctgtgttat cggctcgcac
     4141 gagttttata tgggctatgc actcagccgc aaaaaactgc tctgcctgga tgcagggcat
     4201 tttcatccca ctgaagttat ttccgacaag atcagttccg tgctggcatt tcttgacgag
     4261 atcctgctgc atgtaagccg gggcgtgcgt tgggacagtg atcatgtgat catgctgacg
     4321 gatgaaatcc gcgccattgc tgaagagatt atccgcaaca atttcgagag ccgcgttcat
     4381 ataggcctgg atttctttga tgccagcatc aaccgccttg ccgcctgggt gattggtacc
     4441 cgggtaatgg tcaaagcact tctgattgcc ctgctcgagc ccaccaatca cctgcgcgaa
     4501 cttgaaaaat ccggaaacta cacggcgcgc cttgcactgc tggaagaagt gaaaaccctt
     4561 cctttcggtg cggtctggaa ttactattgc gtgaagcaga atgtgcctcc ggcgggtgcc
     4621 tggatgcttg atatccagcg ttacgaaaag gaagtgctcg ctctgcgcgg ctaatccatt
     4681 catttttact gtttctgtgc gggtattgct tcaggcagtg cccgcacttt taacgctcag
     4741 gaaactgcca tgtccgcatc cgccatacca accgccacgc tgaaagcccg ggaagaacgt
     4801 cgcatccagc ggggacatct ctggtgctac agaaatgaat ttatgtccct gcccgatctg
     4861 gaggacggcg acgtctttga cgtgtattcc tcaggtcgcc gttttgttgc ccgtgctttt
     4921 tatcaggcgg aaggcggcat ttgcgggcgg gtgctgtctt accatcagac agaaataaac
     4981 cgttccttct ggaaaaaatg cctgcaggat gctcttgtgc tccgtgaaca cctgtttccc
     5041 cgatctgttg tataccgctg gattcatgcg gaaagtgacg gactgcccgg actcgttgcc
     5101 gaccgttacg acagcgtcgt tgttctccag tccggctgtg ctttttacag aaggcactgg
     5161 cagagccttc aggagatact gcttgctgtt cctggggtgt cctctcttac ttttgctttc
     5221 agggaagaac ggctcgaagc gggtgcggcg ccggaaaagc aggtgctgga actgaacggt
     5281 ctgcgtctcg gattcttgcc ggcaggggca cagaagacgg ggctttttct ggatcagcgg
     5341 gaaaactggc agctggcaga acgctttgcc ccgggtgccc gtgtgctgga cggctgctgt
     5401 taccacggac tttgggggct tcatgctgcc cggggcggag ccctctctgt tacaggtgtt
     5461 gacagctcgg cagcagcatt ggcacaggct gaagaaaatg ccggaatgaa tgcgtttgaa
     5521 gatcgtttca cttttattaa ggagaaagtg gagaagcatc ttcaggaatg cacggactat
     5581 gacctcattg tgctggaccc gcccgctttt gccaagagca gaaaacagca gaatgccgct
     5641 tttgcgctgt atgaaaatct gaacagcagt gccatgcaga agctgacacc cggcggtatt
     5701 ctgatcagct gttcctgttc tcattttctt gatggcgcgc tttttgatga gatgctgaag
     5761 cgggcagcac gcagtactgc ccggcaggtg cagctgctca tgatgcgggg cgcatcgccg
     5821 gatcatccgg tgctgaccgc catgccggag acttcctact tgaagtgcgc tgttttgcgg
     5881 gtgctgtaaa gcacacccct acgctgatca gggaagcctg ccctgggctt cttcattatc
     5941 atccgcattc cgggtattgt ctgaaaaagt aacctgaagg ggcgtatccg attttaaatg
     6001 cacgtcctgc tgcgggtagg cgatctcaat tcctgcctca cggaaaaggc gcacgatacg
     6061 atggcggata tcgctcgtga tcactgtccc gtccacattt gcgctccgtt ccagccagaa
     6121 atatacggtg aattccagcg catgcgctcc gaatgcggag agcagcactt taggcggcgg
     6181 gtctgtcaga acctgtccgt gatcagagac agcataaagc agcagttttg ccacgtcgcg
     6241 cgtgggcgaa tcgtacgcaa cgcccacatc cacctgtacc cggtatttca gatcggtcag
     6301 tgtccagttg atcacgctgg tttccaggac tttactgttc ggaataatga catcatagcc
     6361 gttaaacatg cgcagccgtg aactgcggcc gccgatgtcc gtgaccgtgc cggttttccc
     6421 gtctatttca ataatatcgc cgagccgtat gggctgttcc cccatgagga tcagaccgct
     6481 gagaaaattg ttgatcagat tctgagcgcc gaagcccacg cccagagcaa cagcaccgcc
     6541 cagaaaagca aaaatcgtaa gaggaatatt gacataattc agagcgaaat aaaccacggt
     6601 gaggaagagg atataattgg tcaatttcgc aacaatcatc accacattgg agcgcagttt
     6661 caggcggttt acggcgtaat tgcggatgat acgggtgacc atgcggctga caaccagtcc
     6721 caccaccaga atggcgagca tataaaaaag tttgcgcccc gtaatggact cttcgccgat
     6781 gacacccagt tcatgatcga aggcaagcaa tgtcgtctct ttggctgtaa acagccagtc
     6841 cttgcccacc tgcacccacg accgttcttt ctggagaatg cgcgcttctt ccacaatccg
     6901 gtttgcgagc gtgatcactt ccacaagccg cagctgcatg cggttgcgca ggcgcagatt
     6961 ttcctgcagc gattccagtt cacgctccgt cggctttttg cttccctgtt cctctttcca
     7021 ctccgccagc tgttgttcca gcgtattgat gcggctgcgc agactgatgg agcgtctttc
     7081 tccggcatcc cgctccttat ccagaactgc cagtcgttcg tgaaaaatct gaaccacttc
     7141 gcccagatcc acccgcacct tcgcaagagc cggattcgca attctgaagc ggagctgcca
     7201 cagtttctgc acataatctt catactccag caatgcttcg attccggcaa tgcgtgcgcg
     7261 tccggcgtcg atctggcggc gcagatgatc cagatcattt tttgcctgtt gctgggcttc
     7321 gggatcggtg gcacgggcta gagcgcgttc ggcagtgagc attctgtttc ggtgctgatc
     7381 aatggaatcg cgggctttcg ccgcctcgct ctgcagtgat tcaatcagtc cttgctcgcg
     7441 ctttatgatt tcgtccagct cttcctgccg gaaaagtgtc tgcacccgtg ccatttccac
     7501 ttttttccgg gcgaaggcga gctccccttc gagcagctgc agctgccgtt ctttgcgctt
     7561 gagttccatg gcggccgcct gatattcggc ttcgcttgcc tgtacctgca gcttggctgt
     7621 ctcgctttca aactggaggg cagcgcgttc ttcctggggc gccaggcgga gctgctccgc
     7681 cactctgttc agcgccgctt tggcctgggc aatgtccgcc tgctccatac ctgccagctc
     7741 attataggcg cgtaaagaca gcttttcagc atcaatgtcc atctgcttca gtgccgcctg
     7801 atcgcgcagc ccgtctaaaa aagccatgga ataaggaggg tcttccgtca aacgggtaaa
     7861 ggatttcact tcctcgtcaa aaagagcctg ccgctcctgc aacgcctcgt attctttcag
     7921 cgcattcaca tggcgctgcc aggcggactg gagtttacgc agcgtactca gccgtccctg
     7981 aagcgctgta aaggaaaatt cggtaatgcc gacagcgctt gctgattcct gttctgccca
     8041 ttcctgaatc tgaatctcca tttcactggc gcgtgtttca agattcagaa tcagctcctg
     8101 atttttcgca ctttcagaat cggcggaaac agcattttcc tgcgttgccg tttccagggc
     8161 tgcggtattc tttttgtccg gcgcagatgc cggagcgccc gccgccataa tccctgtaag
     8221 aaaaagacac agaaaaaaaa gaaagcgagc cgatctgttc atggtttgcc tgcctgctga
     8281 ttacattaag gaaataaaaa acggctgtga aaaagagggt gacgctgtcg ggcttcatta
     8341 tcgcaacccc ggcagacaga gtcaaccgga ggatgcttat gccacgggca tgacggcaga
     8401 cccggtcatg cccgcatgaa acaggctgaa tctgccatgt cggaaaacac ggtgaggagg
     8461 cctttatttc gtctcctcgg tgatgcgctt gagagaggcg gcgcgggcaa cccaggcatc
     8521 atcgccgtaa agccccgttt gcgaaagatc gatgggcttg aaccccagtt tcagcgcagg
     8581 agaatcttct ttcaaggtaa aattgcgccc tttgatatcg acgaaaaggg gatcggcaat
     8641 aatggaatct ttgtcctcgc cgcttcgctg ccaattttca aacgactgtc cttttatctg
     8701 gatttctctg cctgatgcat cccagtagca gttgcggttt atgacaaagt tacctttgct
     8761 ccaggcaccg ctcagcaatt ctccctgatc aaagtaaact atgttattct cgaagaagaa
     8821 agaaatgtgc gtttcatctc tggagcggaa cacctgttcc ttccgggcga aggcaaaaat
     8881 gttgtttacc actttatttt ccctgccgta gtgctggtgg aagccgctgg ttgtggtgtt
     8941 gcagacgatg ttgtccgtaa gcaggatgtg cgtgctgcct tcatctgtat aaagtcccca
     9001 cccgccggaa atatcagggt tggacagaat gtcatggatg taattgttgc gcagctccgt
     9061 gcccggtgat acgcccagcg tgtaaatgcc gcccatatca cttagctggc ctttacccag
     9121 attgtgaatg tgattgcctg taattttgtt gtgatgcgct gaagtggggt cgtagcccca
     9181 ggaccagccc acggagacgc cggaatagcg aaaatcacaa atctcgttgc gggaaatagt
     9241 attgtgtgag cttcttccga tccatacgcc aacggcactg cggtagatgc gtccgccgtc
     9301 atggagcagg caattatcca ctgtattgta ctcggtaagg cagggtacca gcttcgcatc
     9361 aaatgcttcg cctatgcgca cgccacctgc acccagatca gtgaaggcgc accgttccac
     9421 ccggttgtct ttgcagcctt cgcgcagcca gagcccgtag ctgcccacat gagatacctc
     9481 acaggactca aaaaggcaat ggcgggcgcc ttttgcctgt acagccgctt ccacgctgat
     9541 ctctgcctgt gcgtcgctgt gcccggcggc gccaatgggc cagtctccgt aatacatatg
     9601 cagcccgttg aaatggagat aggagacgaa cttttcttca gcagggtcac cctgaagaat
     9661 taaaaattgc cgggtcagcg gaataacggc atccactgtt tccggagttt ctcctgttct
     9721 cggcaggtag tgcagctctc ctgcttttct gtcaaggcac cattctccgg gcagggtaag
     9781 cgcctccatc aggtgctcta caaaatacca ctgcctgtct gtccattgcg caaagggcca
     9841 gcgtgcggca ccggtaaatt caaggattct gctttcaaaa tctgctttct tgacccgatg
     9901 catcgaggtt gcccaggaat gaaaaactat aaaaagtgct tcatcgagat gttcccaggg
     9961 ccggatgtca ccttcatgaa agcgcagttc tgtactgctt ttaacttcct cgtttttgtc
    10021 ggctgctttt ttaaagaccg gtccgtccgt gtaaaaaaaa tcagaggcgg ccgggtaatc
    10081 accggcggtg tgtgccgcct tgggaaagcg tgcagagtcc agcctgtcgc cattcaccca
    10141 gaaagcgcct acggaaaaat cgcttccccg ggcttcaggc acggaagcga cccagcggtc
    10201 ttctctcagt tcccaggacg tgaggcgtct tccgccgaat agcaccggaa cttctccggg
    10261 gtaagcccga tacacgacag gtgcttcctt gaacccggaa tcttccggcg tgaaaacaat
    10321 ggcttccgta atggggtact cgccgtcgcg gatcagtact tcgacgggta ttttttcatt
    10381 gccttctgta gcgctgcgcc atttccggac ggcatcccgg gctccggcaa tcgtcgcaaa
    10441 ggcaccgtct tcaccggaat cggcggggct ctccagttcc ccggaccagg tgtcgcgtcc
    10501 gtccgtcgac acataaaagc gcttgacctc cctttcggca aatgccgatg cggataaaaa
    10561 aagaacacaa cagcacaaaa gagttaaaat agtacgcatg gctgatattt cctgttagac
    10621 ggacttaaac aacacacttt ttgtgagtat aaaccgaatt taaagagaaa tggtaatacc
    10681 catttcataa aacgaaggtc ctctggatta caacccttta acttctattg attttttaac
    10741 gttgagcacc tgctgaaaag gcataaacac gaaatttgcc gagcgcttct attttccggt
    10801 atactagggc tcctcttcat gtcggggtgt agcgcagtcc ggtctagcgc gcctggttcg
    10861 ggaccaggag gtcggaggtt caaatcctct caccccgacc atttttcttt tgcgtgcttt
    10921 ttgcaagcat caaatagcag acacacaata cccccgcccc ttattcgcct gcttctctcc
    10981 gacatggaca aatcggcttg ttcatgaaat acccgcaacg gtgcctcttc ccgcatacca
    11041 gaatgaggca gagcatttcg ttttgtccga ccggcaggca ggcgctgtct tttcaggcgt
    11101 gctctttcaa aagcggagat ctccccggtg tttttgttta tctccctgaa aaactgctat
    11161 cttagtggaa cagaattccc gctgtctttt tcctttcccc gaaaggaagc caggggccgg
    11221 atagtgttgc aaagatcccg ctgccgtaat cgattcacag cctatcacgc aacgctttag
    11281 actgagataa tgcttaatcg aaaggatgcc gtcatggtat tactggaaga ggtgacccgg
    11341 aggctgggcc ccggggttca cttttcctgc gagaaatttt atgctgcacc gggctccagg
    11401 acagccctgt gggggcccag tggctgtggc aaatctacca tgctcaatct gatcacgggg
    11461 ctgctccgcc ctgacagcgg cacgctccgt atcgacggag ccgatattac cgctttgtcg
    11521 gaaagccgga tggacgcttt tcgcggggaa cggatgggtt tcgtatttca gaccttcaat
    11581 ctcctgcctc cttttaatgc cttgcagaat gtgctcctgg gcatgcgttt ttccgatacc
    11641 atccctgaac aggaatggaa aagccgggca gttgccatgc tgaaccgtgt agggcttgag
    11701 cgacgcctgc acagcaaacc cggcacctta agcgtcggcg agcggcagcg tgtcgccata
    11761 gcccgtgctc tcgtcaataa gccccggctc attgtcgccg atgagcctac cggcagcctg
    11821 gatccgaaaa cggcggcatc ggtaatggca ctcctgctgg aactttgcga agaagagaag
    11881 gtgacgctgc ttctggtcac gcacgacagg ggcattgccg aacagctgcc cgatcttttc
    11941 gactgcaccg gcctcgccag ggaggatgcg tcatgagtct ctggagtatc gcctggcgtt
    12001 atttatggaa ccggaaactg acgacagcgc tgacaattct ttccgtggcg ctgggtgtcg
    12061 gcatgatttc tgccgtcctg acactgcgcg aagagacgca gcgccgcttt gaggaagaag
    12121 ggcaggcctt tgatattgtg gtaggcagca agggtaatcc gcttcagctg gttctgagca
    12181 cggtctattt catggatgcg ccgacgggca atatggacta tgctgttttt gaatcactga
    12241 aaagccacga agatgtgacc catgcttttc ccattgccat gggtgacagc tataaaggct
    12301 accggattgt aggcacttcc cgcgagctga tggactttgc gcccggtgcc cgctccccct
    12361 ataccctggc ggagggacgt tattttgaga aagcacagga agcagtgctc ggcgcacagg
    12421 ttgcccgtga ctcaggactg aaggttggca gtgtttttgt gggcacccac gggctggtgc
    12481 agtcttcctt tgccgaatcc cacgacgatc atccttatac cgtggtgggc attcttaacc
    12541 tgtcgggctc tcccaacgat cgtgctatct tctgtgatat ccagtcggtg tgggatgtgc
    12601 acagccattg cgatgccgat catgaccacg acagcgacag cgaccacgat catgaccatg
    12661 cagaggatcc ggatcatgat catgatcatg cgcatgatca cgatcatgag gggcaggaag
    12721 tgactgccgc attgatcaaa ctggcgagtc ccgcccttcg ttttgagttt aaagaccaga
    12781 tcaacaagca ttccaatgcc atggctgcca ttcccatcat ggaaattcag aaactctatg
    12841 agcagctgct cggtacggca aaactggtgc ttcttgccat cggctatctg gttgtcgcca
    12901 tatcgtctct gtccattctg atcggcctgt atatggcgat tttgcagcgt cgccgtgacc
    12961 ttgccatcat gcgcgctctt ggcgcaacac ggggcgaaat cttcggcgct gtcattattg
    13021 aagccttctg ggtaacggtt ctgggcattg cggcgggctg gttcatgggt gcgctgacct
    13081 gtgttgtgct caaccatttt ctggtgacac gcatcggctt tcatgtgccc gcgattcata
    13141 tgactgccga tctggtttcg gcatattccg ctgtggtgct catggggctc atagccggca
    13201 ttctgcccgc ctggcaggcg tatcgcagcg atgttgcccg tgatctcgcc gagctttgaa
    13261 gagaagaagc ctttttttgg aaacgattga aaacttttat cattcattca ggagtgattt
    13321 tgcatgagaa agcgaaccaa acgggacctt accaccctga tcgggctggt tgtcattgtg
    13381 gcaagtgttg tcggcgtaaa cggctatatg cgccgtgcgg ggcttcgtga gacctatgaa
    13441 aaaatgcgtg ccgctctgga agcgcaccac cgcagcgagg gcgtggcgct gatcgactgg
    13501 aaagagctgc aggctgttgt gggcaagcgc aaaaccggtg cgacttttcc ggcgtcgctg
    13561 aaggaaaagg acggcagact ggtcaatatc gtcgggttta tgaacgccat cgatcaattt
    13621 cgcaatgtac aggagttcat gctgctgcct gtaccgctta cctgctattt ctgtgatgcg
    13681 ccgcccatgc gcgatattat cgaggtgaaa ctggacaagc cagccgacat ggtcaatgag
    13741 cctgtgctga tcggcggccg tctgcgtctg catgaagggc cgggaccact ctttttttat
    13801 accatcgaaa atgccaaatg gaatgaagcg gttcaggatg aggaaatgac ggaaaaagtc
    13861 accgaacagc agcatcgcct gcatctgatt gagggattcc gtgagctcaa aggggagtcc
    13921 gctctgctca atgaatcggc tgaagaagag atgcttcctg gttacacgcc gccgcagcat
    13981 actggaacgg aagaataagc acagcgttcc cggctcagtg catatatccc agctggcgca
    14041 gctgttccag cgtttccgca tccaccgtaa cttcgctctg gacacctgtg cgccttcgct
    14101 gattttcagc acgctggcga tgcagcagct gcttcatggc ggcgaccgct tcgggccgct
    14161 cttctgccag attctgctgt tcttccgggt ccgccttccg gtcgaagagc aggacctgct
    14221 gcttccgctc atccacaacg agcttcagag tctccgtgag tacggcatcc atttcgatat
    14281 tccatgccgg agaatcggat tgagtccggg cgaagccgtg ccgtgcctcc gccgtaaaaa
    14341 gatctttccc ctgccaggat tccggcgcag gcagtcccgc cagccgcagg agtgtcggcg
    14401 caatatcgat ggtctccaca agacggctct gctctccttt gggaacgcct cctccctgca
    14461 gaatgagggg tacctgcaat tcgcaattgt acaggctcag gccgtgtccc agcaggttat
    14521 gatcccagaa atgctcacca tgatccgcca ttacaatgac cagcgcatca gacgatcgcg
    14581 cccgcacacc ctccaccagt cggcgcactt cctcgtcaac actgcgtact gatgcatcat
    14641 agagaagccg taccgcttcc ttgccgtctt ccgacagctc ctgcaggggg aaggtttcag
    14701 aacgtccggt agaaaaatgt gcccacgcca taacgtattc caggaaatgt tcaacggcca
    14761 cctgttcttc agcggtcagt ttttccggtg cgtatcccag aagcttccgg tattcctccg
    14821 gtgactggta gggcaagtgg gtatccatgt aatgcgcata gaggaagaag ggagatttta
    14881 acgacgggac agctttcaga gattcctgag tgacccagct gcccggcgca tcaagagagc
    14941 aggtatagtg atcaaagccc cggtcaaagc ccatgacggg gaagaggttt ccgttggtct
    15001 gcacgcctgc cgttgcatag cctcctgcct gaagcagtgc agccagaacc tgccggtccg
    15061 gaggaagagc agtctgcctg ttgccggatg cctcgtcttc ggcggcaaag acaacttcat
    15121 gagtttccat gtaactggag gtaaacaacg atgccatggc aggccgggtc caggtgcatg
    15181 cagtgacggc atcaggaaac cacgcaccct ccgcccggag ccggtcgaga aaaggcgtta
    15241 agggcacacc gttccgctca gcacccacgc gatccgcccg cagcgcatca agtacaatca
    15301 gcaccatgtc cggcgcgtga tcggctgctt tacgggcagg aaggggcctt tctgtcgata
    15361 cagcggcatg ttccccggtc aggacaaggt acagaccgag cccaaggagc agcagtgcca
    15421 ccagaaggac acctgccatg ggaagccaca tattttttga ttgagcctga ttcataaata
    15481 ctcgcactag aaggtccgaa aggcgtaaga caacggagaa agtcctatta taacagcctg
    15541 catcgtgcat acagtaggct ttgtatcccg ttaccctgaa aggcgctgcg ggaaggcgtg
    15601 cgctcccgga tattaagata atttccctgc ggcttcgaga ctgtgctgcc gatgctgtag
    15661 aatatgaaaa agaacaggca tccgccatgt cgaaagaagg actttacgca tcagccgaca
    15721 ggagtgttat gaacaggtat ttttccggcg gtacaatgag caggcgcgca tggcttgccg
    15781 ccagcggcgc ggggcttgct gccgcctttt caaattcaaa actttttgct gccgacgggt
    15841 caccggcgcc gacagaggct ccacttccca atattgttgt atttttatcc gatgcccttc
    15901 gtgcggatca tctgggctgt tacggctatc cgtttcccgt gagtccccgc attgatgctt
    15961 ttgcccggga aagtctggtt ttcgagcagt gctattctca ggctccctgg acaaagccgt
    16021 ccgtttcttc tttatacaca ggtgtattgc ctgtggttca tcagtccgta gtcaccagtt
    16081 ttgagcagga gagtttctgg cattgggacg agacaaatcc gcagggcttt gagatacaga
    16141 ttctgcgcga gagctttacc actctggcgg aagccctgaa agcaagagga tatcagacag
    16201 cctgtttcca gtatacgccc cattgccaac agggcttcgg atttgcccgg ggctttgatc
    16261 actaccggta tgtgccggtt gaacctgtcg taccgcagac agatgccctt atcaaatggc
    16321 ttcaggaaga agcaaaagag ccgtttttca tttatgtcca tgaactggat cctcacggcc
    16381 cctatacccc cggggaggcg ctgtacgaga agcttttcgg aaaaacactc cagcgggccc
    16441 gggaagaact ttctgaattt gacagagcaa tcatacagaa gtttcaaatg ccggatttat
    16501 tgcatctgag ccgggaggga ctggagcatc tcataagcct ttatgatgcg gagattctcg
    16561 gcgtggatga gcagttcggc cgtgtgcttg actgtcttca gggaaaaggc gtggagggac
    16621 atactgtcgt ttctctgatt tcagatcatg gggaagcctt cggagaacat ggttttttca
    16681 agcataacaa ctctctctat aaggaagaac tgcatgtgcc gctgatcata cggacagggg
    16741 aggggagcca gcaccggaga gtgccctggc gtgtcagtct ttctgatctt tatccgtctc
    16801 tgctgcagct ggcaggtgct gctgttccgg actatgtgca ggcagctccg ctttttgatg
    16861 cgcagggcgc tctcacggta acagagagcc gccccgttta tgcctatttt gatcataatc
    16921 gcccggatat ttcctcctgg gagttctgca tgacagacgg ggatctgaaa atttatgata
    16981 aaggcgatgg aaagcctccg gaaatttatc atcaaaaaga agatccggac gaaaaacgga
    17041 acatagccga tgcactgccg caggcagcgg aactgcagga aaaaataaaa tcagcccggg
    17101 caaagcatga ggagttatcg gcacgtttcg ggccgcctca gtatgccggt atcgatatga
    17161 aatcaatgga agaactgcgt gctctgggtt atttttaagt ttctgcccgg gacatcatca
    17221 tgaaagagat ccctcaaagg aatagaaatt tgtgtatgcg catgtttcgc tgtctgactg
    17281 atgcaggctc ttcaaagcag gtatgtctcc ttcttttgat ttgttttatt gcagcagcgc
    17341 ttcgcttata cggcctggcg ggcgaagggc tgtggtacga tgaagggtgc agtatttatc
    17401 tcagcgacta tgcatcccgc tgtgaccgcc tcctgaacat ggattactgt aatgatccgc
    17461 cgctgtttct cgtgctgctt ctgggctggc gccggattct atcttccatt tccgggagcc
    17521 ttgccacaga ttttatgctg cggcttctgc cctgtttctt cagtgttatc acggtgcccg
    17581 ccgtctatgg cgcctttcgt ggactgctgc aataccgcac gactcccgaa acttcgccgc
    17641 aggatagccg ttttgcggaa ctgtccgcac tggcggcggc ttttcttgtg gcagtatcac
    17701 ccttccaccg ttattatgct caggagctgc gttgttacag cctgcttact tttcttgccg
    17761 tgctggtact gtggaatttc atcgctgcat tgagagacaa ccggctttgg caatggataa
    17821 cgcttacgct tcttctgagc ctctctttgt ggacgcattt tttttccgcc tggctttttc
    17881 tggtgctgga tctgtatctg ctgcttacgt tcagagcgca taaaaaacgc tatattccct
    17941 gggcgttgtg ccatatcgcc gcacttctgc tgagccttcc tgcgttgctg ctcgcctggc
    18001 gcatgtctca ggtggttgcc gccattgccg tccagtggat cccgccgccg ggactgaaga
    18061 ccctttttat cacgttcaag actttttttg cgggttacag cccccgttca ctcgtctact
    18121 ggcccctttt tctgctcagt gccgggcttt gccttttcgg tgtccgggcg ctgcgccaca
    18181 gcagacagaa cctgcttctt ctcctcttat gggctggatt gcccatggtc agcagcgcag
    18241 ttctctggcg gattcgcgac ttttcttatt atgaacttcg tctcttcatc tggtgtgctc
    18301 ccgcagctgc ccttctggcg gcatgcggct gggcatcaat gcgcttccgt tggcgcatgg
    18361 tcatcggcag tattttcctg ctcctgacgg ggatgctgct ctcggacagc atcgcgcatg
    18421 cccttcatcc tgtgccgcag catcggcttg gcgtacgcta caaagtggat aaccgcggtg
    18481 ccgcccggta tctggctgct tatgctttcg ccggcgaacc tgtctatcac agcagtcatg
    18541 tgacgctgcc gtcttttcgc ttttatctgc ccggtcacga gcatacccat atctgcctta
    18601 ctcgggacga ccgggaaggt tttttcagcg cctatcccaa tcgacccctc tggacgcatc
    18661 tcaatttcca gccgacaccc tggcatgctc ttgcgtcttt tcctcgttct ttctggcttg
    18721 ttcgatcctg gtgggagcct tttgaagtgc ctgaaaatct cttcctcctg gaaaaagaaa
    18781 tagaacagca gtgcgttcgg gaagaagaac tggctttttt tgggattacc ctcattcact
    18841 accagcgcag gggtacgccc tccggacagg aattcttaca atgaaagaga atggggagag
    18901 aaaacgttcc cggatgtttt tattctgcgc aaaagcgttt tcagtgattg caggtgtttt
    18961 ttcttttctg ttggtgtttt tcctgtgctg ttttatgatt gattcttttt tccactatat
    19021 cacgcccgca gaaaaatata aattggtgga agaaaaagcg cctgtaaaat acgatcagaa
    19081 ggcaggtttt gtcattaacg atgacggaac gtataagcgc agttttgttt tcagggacgg
    19141 cagtccgcct ttggattcga catttaccct gcaaaatggt atgcgccaca caccggtgtc
    19201 cggggagaag cgggataaat tcgccctttt cttcggcgac tctttcacct tcggggtagg
    19261 ggtcaatgat acggaaacac tgccgtatta ttttggagaa gaacagcccg ggtacatgcc
    19321 ctacaatttt ggcatccccg gcggctctgt ccagcatatg tatctgaaac ttcaggggaa
    19381 ggaaatcgaa gcaaaagtaa aggaacagga cggcattgcc ttctactggt ttttcggttt
    19441 tcataccaga cgagccgtcg gcgggatgcc ggcatttaac agatgggcgg atatgatgcc
    19501 ctgttttgaa ctggaagaag accagctggt ttataaagga aatttcagag atgcgcatcc
    19561 ccggcgtgcg cttttatatg atctgctgta taaaagcagt actgtccgct acctgaacct
    19621 gtttttgccc cggacctttt cagagtatga ttatcaggta acagtacagc tgctgcttga
    19681 gtcatatcgg ctgtttaaag agagctgccc gaacggaaaa ttcatcttgc ttatgtggga
    19741 caaccgggat tcttataccc ctgtccagaa aatgatcagc gaggcaggca tacctgtcat
    19801 tctgataaag gagttttttt tcggtgttga taatcctctg gaaacaggtc agaccgtgcc
    19861 ggatggacac ctgacaggcc ccggctatca acggattgcc aaaggattgg cagcccatag
    19921 cttgctttcc gcaagcgcag cgtatgaaaa aaaataaaag cgttctgttt cgctcaaaac
    19981 aaatgcctga tcagacccgg ccacaggctc ggactgtaaa gtacattgac cgacatggcg
    20041 cattcaaaag aacagtggca tttactttct tttatatgcg caacaattgt gcgggctttt
    20101 tctgactgca ggattttacg gatatcgtaa tcattttccc gcatgctccc catgaccgct
    20161 gaatcgaggc tgcttattcc tgttttgata aaatattcca gcacttcgca ggggctgatt
    20221 ctgccttcat catccataac aatcatattt ttaccggcac ggcagggcgt gacaaaacgg
    20281 tcttcgacaa cgctttgcat gatcagatcg gaagcctgcc gggtgatggc agtgaaaagt
    20341 tttcctgtcc agcttttccc ggtggctttt tgctccgttt tttccctgca ggcacggacg
    20401 aaggcggcat aagcttcgtt attgattttc gccgcttcag gatcgtgggt gtcgccgcgt
    20461 acatagaggg cgccaaagtc atcaaaacgg aaagtacgat ggacatactc cagaatgtcg
    20521 tattctttcc cctgattaaa cttggaaagc accattgcca tgccgacact gagattgggc
    20581 agctctttac gcagcagtgc cagttcatga ttcagtgttt tgacgcattc gaaaataccc
    20641 gctttgccgc ggattgcatc gtgatcttcg ccgataccgt ctatgctctg tgtgatgcgc
    20701 acccaggcat ccggacagct tttacagatg ctgcggactg ccgggatgat ccggtcggga
    20761 tgagacgagt tggtggggca ggcaaaaagc cgggttccgc tgtgcttgta aaaaagctca
    20821 gggattgcag caaagtcctt tcgcagaaaa ggctccccgc ctgaaaagtt tacatggtgg
    20881 agtccgggaa agttgcgcgc tatgcgctct atctcctcaa tacgcagcgg tgagcgatgc
    20941 gccgcatgat cgatcacttc gcggttgaag cacatcacgc aacgacagtc gcacaagggt
    21001 gtcacaaaaa aaatcatata gccgggagtg ctgcggttga gatagccccg tgccagcgtt
    21061 gcgaatcggc cgctcattgg tatgatcctt tcgtgaaccg ctgaagcagc cccagcccgg
    21121 cgaaaacacc cataacaagt gtgtgcaccc agcatgtcaa tgctgccctt aaagaaaaca
    21181 gcattccttc ttctttccat gcccggcaga tgaattttcc gtttcctgtg aaataaagca
    21241 ccagaa
//
LOCUS       DAOPWB010000048        21125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2492_length_21125_cov_4.250024, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000048 DAOPWB010000000
VERSION     DAOPWB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 21125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21125
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_21125_cov_4.250024"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="PKN92_06235"
     CDS             <1..1348
                     /locus_tag="PKN92_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNZ48427.1"
                     /translation="MIGLPTEQEEDVDAIADLCIRTLGTAKKIRSRAELRLGVSTFVP
                     KAFTPFQWAAQIGLEETRAKHRQLAALLKPWRAIRFGRHAPESSFIEGMLSRADRRAA
                     DLLEAVFHRGGGYETWEEKLNFALWTEAAAAVHYPVEDFFRERSEYFRFPWDHLEGAV
                     EKKALWKEWQGALTESATPDCRQGKCHLCGAIKRAPECCAVVLDTHKRAETAEILVPR
                     EPVAVKEAVQRMRFRVGREGRLRFLSHLEFAHAWIRGLRRAGAPLAYSQGFHAHPKVT
                     FSSALPVGEESRAEWMDVLLTEKTEAAAFMEKLRAVMPEGLQVYEAFEADMGDPALMA
                     AVCGHRYIIRTPLPPDALAERIARLEAQDMWHVNRRVKSRASREGKKAILLDLRPLVC
                     ALGMWKEEAETGTGSSFFFDTTLFAGRLAKPREMIALLGFEASEVRIVKQTTLLSQ"
     gene            2109..3923
                     /locus_tag="PKN92_06240"
     CDS             2109..3923
                     /locus_tag="PKN92_06240"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNZ48428.1"
                     /translation="MPRLKRQVILCEARHCGTRGDEDIFTVFQDEINRAGLDPEENTL
                     IHVGALGFCSIHRKMISTCVMVRPENTVYFVKDRKDVQEIVAQHLVEGQIVDRLRCQE
                     DPISLRFFDLYGDAAFFNRQTRISLRNAGVINPESLYEYIHYNGFRALSLSLSNGDPD
                     WLVERVLESKLRGRGGGGYPTGRKWKDAHRHANDQRYLICNADEGDPGAFMDRSMLES
                     DPYSVIEGMIIGGYAIGATRGFFYVRAEYPLAIKRIEKALAECRKHGLLGQNILGSDF
                     SMDLEVRLGAGAFVCGEETALIHSIEGERGQPRIRPPFPTESGLWGKPTVINNVETFA
                     NIAPLLLYGSDWFASVGTEFSGGTKVFALAGKIQHTGLVEVPMGTTLREVVYNIGGGI
                     PGGKQLKAIQTGGPAGGVIPAEYLDMPIDFDTLGRLGSIMGSGGMIVLDEDDCMVDVA
                     KFFMTFCQGESCGKCTPCREGTKRMLEILERITMGKGEMEDLDKLERLASLVKNTSLC
                     GLGRAAPNPILSTLRHFRDEYEAHVKEKRCPARKCKTLIHYEIDPAKCVGCTMCARNC
                     PTNCISGGRKEVHVIDQVECIKCGRCFDVCRFDAVNRK"
     gene            3987..5741
                     /locus_tag="PKN92_06245"
     CDS             3987..5741
                     /locus_tag="PKN92_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNZ48429.1"
                     /translation="MVTLTIDGVKVTVPDTMTIMEAAETQLGIRIPRLCYHPDLSIQG
                     SCRVCIVDVKGIPFYQASCAVKVAQGMEVETNNPAIRQARRDIVELLLDNHPRACLTC
                     DRDGNCELQNVAYATGVRERLFAGERKRFPIENTNLAVDRDPEKCILCGRCVRICDEV
                     QGVHNLSQQGRGFHTLVAPAHDACMDDSVCIQCGQCVAACPTAAFTEHDHTEEVWKAL
                     ADPTKHVVAHTAPAIRATIGEGFGMPLGTPAAGKLVTALRRLGFDRVFDTNFSADLTI
                     IEEANEFMQRFSKNERLPLLTSCSPGWVNFLEKFYPELIPNASTCRSPMAMMSSLLKT
                     YYAEKTGIDPKDIFVVAIMPCTAKKFECNRPELCMPDGTRETDAVLTTRETIWMIKNY
                     GIDLRHLPDGEFDSPLGESSGGADIFGTTGGVMEAALRVAVEKVTGQAPENMEFTEVR
                     AVEGLRERELKVNDELTLRVGVANGLTNAKQLLDAVVKGEKEYHIIEIMACPGGCIGG
                     GGQPYPPEGYRILDKRLLAKRAEALYKIDQGKKVRSSGHNPAILNLYEEYLGEPGGEK
                     AHRLLHTHYAPRLPRGIR"
     gene            5741..6292
                     /gene="nuoE"
                     /locus_tag="PKN92_06250"
     CDS             5741..6292
                     /gene="nuoE"
                     /locus_tag="PKN92_06250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNZ48430.1"
                     /translation="MDVKVTDNRDEVFSKSKEALTPEIVAFIEDCTQKQHPNSHLINV
                     LHKVQGHFGYLGKEQLDAVAQLLQVPTAKVTGVATFYHFFRLEPRGRFVINICLGTAC
                     YVKGAERVCERLCDELGIHVGETTPDGMFSLEGTRCVGTCGLAPVVLVNDTVFGEVTP
                     DQIPALIESHIKKAQAMEKESVV"
     gene            complement(6323..6826)
                     /locus_tag="PKN92_06255"
     CDS             complement(6323..6826)
                     /locus_tag="PKN92_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNZ48431.1"
                     /translation="MEEEEVPVSPEHVRPSWDEYFMEVANAISKRATCNRGRSGCVIA
                     RDRTLLVTGYVGSPAGFPHCDDVGHQIKQVRHEDGSITEHCVRTVHAEQNAICQAARL
                     GVSLQGATLYCRMTPCRVCAMLLINCGIVRVVCERRYHAGAESEEMFRTAGISLEYLF
                     DEVQPYE"
     gene            complement(6830..7609)
                     /locus_tag="PKN92_06260"
     CDS             complement(6830..7609)
                     /locus_tag="PKN92_06260"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48432.1"
                     /translation="MVSTAFQSPLEKEQIRVALFGKEASSLVPLLQEKTDLVVVDTTP
                     DLVICYGGDGTLLAAELHWPGIPKVPILNSKRGNRCIQRRPEEVIAALSGRDMVRTLY
                     TKLECSIFTGEGVKAPDFSFTCLNEVNVHMGRINSAVRFQLSVNAVPFEDGVEIVSDG
                     FVVCTAFGSNAYFKAITKGIFTEGIGVAFKATTHAVNHIVLPEDSQLHFVITRGPATL
                     AFDNSPEFVDLEEGDRLVIRQHPDSACILTCDPVTKLDEPF"
     gene            complement(7649..8377)
                     /locus_tag="PKN92_06265"
     CDS             complement(7649..8377)
                     /locus_tag="PKN92_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol O-phosphatidyltransferase"
                     /protein_id="HNZ48433.1"
                     /translation="MTSKSSISDIPGAWLVHILTASGALFGLLALQASARGAFVIALG
                     WMAVTIAIDGVDGALARWRRVSVHLPYFDGALLDNMVDFFTYVLVPAYFLLYAPVLTE
                     AWRLPVAAAIIFSSAYQFSQTEAKTADHAFTGFPSYWNIAVCYLFLLEWPPVVNAAVL
                     LLCAGAVFIPVRYLYPSRTPHYRAINILLGLLWAAAVLGALAVYPKGHHPLILLSMLY
                     LIYYFAFSLWLTFRRKSATEKEAS"
     gene            8667..10328
                     /locus_tag="PKN92_06270"
     CDS             8667..10328
                     /locus_tag="PKN92_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNZ48434.1"
                     /translation="MTTLVWLDWLVIGGYFAAVAAVVWFTARQQSTSEDYFLAGRNIG
                     WFAIGASLFASNIGSEHLVGLAGAGAKSGVAMAHYELHAWCLLMLGWVLVPFYYRAGI
                     YTVPEFLERRFNPACRWILSLVSLIAYVFTKVSVTVYAGGMVFQTLIPELQFAGLNSF
                     WIGAVSVVLLTGVYTMVGGLRAVVYTDAMQTVVLLLGSLCITLIGLYHLGGWSVLREM
                     NGSVRFNLWRPASDPEFPWPGMLFAAPIVGLWYWGADQYIVQRTLAARSLKIARRGTI
                     FGAYLKITPVFLFIVPGMIAYALVQKGMLVMDSPDQAFAALVTQLLPQGVRGLVVGGL
                     LAALMSSLSSLFNSCSTLFTVDIYKKLKPAAPESELVRVGRIATGVVVGLGLLWIPAM
                     QLVSGALYEYLQSVQAYLAPPMTAVFFLGIFWKRINGTGAVVTLVSGFVLGLAKLGAQ
                     IYAGIAVSDMIMQKPWILRLIIAYGNINFLLFCVYLFLYCCTVLVLVSLLSAAPTAAQ
                     TTNLCYSGNTEAGRREVRESWNYWDVIHTAAVLLLILSVYLYFTG"
     gene            10373..11311
                     /locus_tag="PKN92_06275"
     CDS             10373..11311
                     /locus_tag="PKN92_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ48435.1"
                     /translation="MGRFIAKHSPLFILAALCIILAVVSPVFRSPSNLQGVAQRTAVV
                     GIMAVGQLAVILTAGIDLSVGSIAALSGVVGCLAMVNLSVPAIPGIFIGIGVGLLCGL
                     VNGLLTAKGKIPPFIVTLGMMMAARGAALTASGATPVFGLPENFAWLGGTRGWWLPVV
                     FMFGIAAFFAVVLGYTRFGRALYVIGGNKEAARLSGVPVDWVRIAAFAISGTAAGFAG
                     MMLASRTSVASPTAAEMYELQAIAACVIGGASLMGGEGGAFATIAGALIMTVLYNFCN
                     LQNINVHWQQILVGVLLVALVFYDNRRKRKAGLLKD"
     gene            11348..12328
                     /locus_tag="PKN92_06280"
     CDS             11348..12328
                     /locus_tag="PKN92_06280"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNZ48436.1"
                     /translation="MKIQVMGAGGWGIALARHACRLGHETVLWGRNPEKMQRLARERQ
                     SPELLPGILLPPEVRVVTEPEKTADLVLYAVPSKGMKDTLEQYPFPLSVPRISAAKGI
                     EQDSLLRMSERIALSAPGAPVVVLSGPSHAEEVGRDLPTCIVVAGTDVALLQQVQQSL
                     GAPHFRVYTSPDVIGVELGGALKNIIALAAGACEGFGLGDNARAALITRGLAEMRRLG
                     VACGAEASTFAGLGGLGDLVVTCASHHSRNYRVGLQIAAGRTLEQIQADSVQVAEGIE
                     TARSAWRLARRHAVEMPVVKAVHDTLFEGLDARSAITGLMTRLMKAEWES"
     gene            12524..13204
                     /locus_tag="PKN92_06285"
     CDS             12524..13204
                     /locus_tag="PKN92_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48437.1"
                     /translation="MPYKQILMILVLMLTSFTVLAQPPEAGGDDAALLDPYSNPPSGI
                     KEEKSPELEGDTIVFKSGSELKGIHIIRESALFVEVEYLPGESPVKFPTSQIKEIIRQ
                     DPASHASGNAGAAGSKPDLMPGEEVSAQFHQALMTVLSETPLELTNADYLAVIKDFAE
                     KAEAPLEIKERVTAIPEQERVFTRTLPAGITLLDFVQKEFSEIAPKVRAVLQFDKLIL
                     ELREAPVE"
     gene            13298..14203
                     /locus_tag="PKN92_06290"
     CDS             13298..14203
                     /locus_tag="PKN92_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ48438.1"
                     /translation="MSQPCIYPVELMHRPASLLRIRPKTRGLYHQRMNVVYAEVHGTG
                     LLMDIFAPSGISRGIGVVDVISGGWHSDRAMLHQHIGLGLIDALCERGFTVFAVSPGS
                     VPLFTGQDMVRHIHAAIRHIRQHAVTYGIEETPLGITGVSAGGHLGAMAALTPQKARP
                     DAREPWRQQSTDIQAAACFFFPTDLVDYGGQGFDRLQMDGFNPLELLFAKHEKASIDT
                     ELKLRLQALSPALLPIVSPPPFLLIHGKQDMLVPWEQSEKLASALRAAGGEAALLYRE
                     EGGHFWPDISVEVDAAADWLEQRLT"
     gene            complement(14207..15604)
                     /locus_tag="PKN92_06295"
     CDS             complement(14207..15604)
                     /locus_tag="PKN92_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48439.1"
                     /translation="MKKQIIYTVLIPMLLLFVAAGFPCPAAPSEGSMKIAVFDLDVTP
                     PVGTDLAYDSVAGTWDMSLRARGIVLCGAGDPIVLCAVDWIGIGNESHDAFREVLARA
                     AGTQPERVAVHTLHQHDAPGCDFTTERIALSLGANPKRYESTFQRQVLAELESTVLRA
                     MEKTEPVTHFGLGKAEVYEVASNRRILDENGKVRVTRFTACADPAVRAEPEGVIDPEV
                     SLVSFWNEDTPLAVLSYYATHPQSYYRTGIPNPDFPGVARFLRQLEVPSALHVHFNGA
                     GSNIGAGKYNDGSKENRLILAKRLADGMKRAWDATEKYPLTADAVGWNSVLVTLPTGP
                     WLSREGIEAGFQEQPDPARILGSASKLAWLERQESGHQFDIPCLSLGTARILHMPGEL
                     FVEYQLSEKKQRPDLFVAMAAYGDYSPGYIGTAVAYEQGGYETSEAASNVAPEVEGVL
                     MGAVKQLLGGPPSAE"
     gene            15786..17213
                     /gene="proS"
                     /locus_tag="PKN92_06300"
     CDS             15786..17213
                     /gene="proS"
                     /locus_tag="PKN92_06300"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNZ48440.1"
                     /translation="MSKGITKRSEDYSQWYLDVVLKAEMADYSPVRGCMVIRPTGYGI
                     WEKIQQQLDRMFKETGHVNAYFPMFIPESFLKKEAEHVEGFAPECAMVTHGGGKLLEE
                     PLAIRPTSETIIWSTYKNWISSHRDLPLLINQWANVCRWEMRTRLFLRTTEFLWQEGH
                     TAHATEEEAEEEAFKMVHVYRDFAENYLAMPVMVGRKTEAEKFAGAEHTYCIEAMMQD
                     GKALQAGTSHHLGQKFAQAFDVKFQDKDGELKYVYASSWGVSTRLVGGMIMTHSDDSG
                     LVVPPRLAPTPVVFVPIYRKDEERALVLEKAEALCAGWDKLFYKIDDRDQYKPGYKFA
                     EWELKGVPVRIEIGPRDVASNSVVVVRRDTGAKETVSADTLHSYVETLLNTMQTDMYN
                     TAKKRMEDNTHTVDSYDEYKERVGGGGFYRVFLDHSNPEVEKQMQEDTRSTIRCIPFD
                     APEEQGSCMITGAPCSSRVIAAQSY"
     gene            17238..18179
                     /locus_tag="PKN92_06305"
     CDS             17238..18179
                     /locus_tag="PKN92_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ48441.1"
                     /translation="MTHVNLVTGGAGFIGSHLCEALLERGEEVLCLDNFFTGRKTNIE
                     HLLGHPRFELVRHDLVNPIVLETDRIFNFACPASPVHYQFNPVKTIKTNVMGALNMLG
                     LAKRVGARILQASTSEVYGDPTVHPQTEDYWGNVNPIGPRSCYDEGKRVAETLFFDYH
                     NQNQVDIRVIRIFNTYGPRMLVNDGRVVSNFIVQALRNAPISIYGDGGQTRSFCYVSD
                     LVRGILRMMDTDGFHGPVNLGNPGEFTILELAQKVIDLTGSRSVLEYHPLPENDPTRR
                     RPDISLAEERLGWSPKVPLEEGLTETIRYFDTLLREG"
     gene            complement(18076..18567)
                     /locus_tag="PKN92_06310"
     CDS             complement(18076..18567)
                     /locus_tag="PKN92_06310"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HNZ48442.1"
                     /translation="MAGKFVDFHRREKSVQPQVFESLPDNRSLWYETAPEQAEAERRA
                     RERAVKLEWLRFAVARCLSSAESRAIVLYYFKGYTTRQCARHLRCHSTTVHRRLVRAI
                     RKLTVYAWLHMPEYFTSEGIAAAVREGLIPPEAACRNSGSSLSALPPAALSETSRGVL
                     PPD"
     gene            complement(18710..19288)
                     /locus_tag="PKN92_06315"
     CDS             complement(18710..19288)
                     /locus_tag="PKN92_06315"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNZ48443.1"
                     /translation="MQHRSITTGKGDQGESRALNGSTLPKDHAMMEALGALETLRAHL
                     ALLQIRLQPIAEEKESTDCLFFLLHACFALGAFISDPESSDPESPHALTPQHLERLEQ
                     EQHRLERELNLPGAFIAAASNEDAALADLAVAAARSFERRLVGYQRVAPGFNAPLPLA
                     FANRMSDFLFILARHLEKGVYHTVDYSRLNPD"
     gene            complement(19328..20332)
                     /locus_tag="PKN92_06320"
     CDS             complement(19328..20332)
                     /locus_tag="PKN92_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48444.1"
                     /translation="MKHTFSSFVPSLLFLILWAVSLHCAAQMPPLPEKERLQQGIFTI
                     DYVPSQHKTAVLSLQILEEALKEFSAYLPAGEAPVHVAIADTLDDFDQYAVHFAGLDV
                     SGVAHPGAGLIAVKAPRLREPGGDYAGTLRHELVHLLLFRSIGYDRLPQWLNEGLAMY
                     LSNQHYWDGAMRIAVMFVRNRIIPYHELDNAFYQPLGREEFSDAYVQALYMTRQLHRQ
                     LGEEKFWKVIHALKEQTFGKALISEGGIDLETFCRGYEKSLWKYTIIALTASGAAFQP
                     GAILLILAYLRHRRLSKGLYRKWEEEELEALVDRDPPVFTWNEVVEDPDFWKQDREDD
                     "
     gene            20543..>21125
                     /locus_tag="PKN92_06325"
     CDS             20543..>21125
                     /locus_tag="PKN92_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNZ48445.1"
                     /translation="MIEILSKPSMLSVVVLGASGDLAVKKIFPALFSLYCRRLLPASF
                     HCYGFARTEMSESAFRQLLMSHLTCRYTPGETECHDLMEGFLGHCSYVQGNYDDQASF
                     VRLQRELEKREHKDGTTKRIFYLSVPPFIFLPASKTLHETGLLAEIPGTLQCRLVLEK
                     PFGRDRASSDALTKDIAALCDESQIYRIDHYLGK"
BASE COUNT         4743 a         5649 c         6087 g         4646 t
ORIGIN      
        1 catgatcggg ctgcccacgg aacaggagga agacgtggac gctattgccg atctgtgtat
       61 ccgtaccctc ggcacggcaa agaaaatccg atcccgggcg gagctccggc tgggcgtatc
      121 cacctttgtg ccgaaagcat tcacgccgtt tcaatgggca gcgcagatcg ggctggaaga
      181 aacgagagcc aaacaccgcc agctggcggc actgctgaag ccctggcgcg ccatacgctt
      241 cggaaggcat gcgccggaaa gctcctttat tgaaggcatg ctgagccgcg ccgacaggag
      301 agcggccgat ctgcttgaag cggtatttca ccgcggcggc ggctatgaaa cgtgggaaga
      361 aaaactgaac tttgcgctct ggacagaagc ggcagcggcg gtgcattatc ctgtcgagga
      421 ctttttccgg gaacggtctg aatatttccg gtttccctgg gatcatctgg aaggcgctgt
      481 ggagaaaaag gcgctgtgga aggaatggca gggtgctctg acagaaagcg ccacaccgga
      541 ctgcaggcag ggaaaatgcc atctgtgcgg tgccatcaag cgtgcccctg aatgctgtgc
      601 cgtagtgctg gatacacata agcgcgcgga aacagcggaa atactggtac cccgggaacc
      661 ggtggcggta aaagaagcag tgcagcggat gcgcttccgt gtgggcagag aaggcagact
      721 ccgtttcctc agccatcttg agtttgccca tgcctggatc cggggactgc ggcgtgcagg
      781 ggcacccctc gcctactctc agggatttca cgcccatccg aaagtgacct tttcctccgc
      841 actgcctgtc ggtgaggaaa gcagggcgga atggatggat gtgctgctca cggaaaagac
      901 agaggcagcg gcatttatgg aaaagctgcg ggcggtaatg ccggaaggcc ttcaggtata
      961 tgaagctttc gaagcggata tgggtgatcc ggcactaatg gcggcagtgt gcgggcatcg
     1021 ctatataatt cggacgccgc tgccccccga tgcgctggcg gaacggatcg cacgtctcga
     1081 agcgcaggac atgtggcatg tgaaccgacg tgtcaagagc cgggcatcca gggaagggaa
     1141 gaaagccatc ctgctggatc tgcgccctct ggtgtgtgct ctgggcatgt ggaaggaaga
     1201 ggcggagacg gggacgggaa gttcgttctt ctttgacacg acgctttttg cagggcgcct
     1261 ggcaaagccc cgggaaatga ttgcgctgct cgggtttgaa gcatcagaag tgcggatcgt
     1321 gaagcagaca acgctgctta gccagtaaag cagaaccgct cccccgcttt tgcgcagctg
     1381 tccttattaa atggagccag ttgtgacgta cgtcacagca ggagaacttg cgccgccctc
     1441 tgcttgttct gttccggcct tcgccgaagc gttatcacct aattgctgtt atttcagcaa
     1501 gttgcattta gttgtgaaaa aaataacgat tacgcgccac gaaagttgac tgttacgatc
     1561 gtctaaggta tactatgagc gtatagaaaa agagcgtgag gtgcttttga gcaattttcc
     1621 cgcacgcata gcctgcaacg cggtactgcg gttctttttt ttcgcactac cgtcattcga
     1681 tacaccccct tattaataaa cgcattccca tcaccgttta tttcaggagt aaggcaacac
     1741 ttatgaacaa cactgtttta agccgttatg aagaactggc acagcacgcc cacactcagt
     1801 ggcagcagtc gggccggaat gacaagatca tcattcaggt cggctcagcg acctgtgaaa
     1861 atgccgccgg agccacagag gtatggcgcg aatttgagcg caatattcag gcatcgggcc
     1921 gtgatgatat tatcctgcat caggtgggct gcacaggccg atgcagccgt gaacccatcg
     1981 tgggtgtcct gatgccgggc agaatgcctg tgaaatacca gcgtgtgacc cgtgactcgg
     2041 cgcatcagat tttctcacgg cacattcagg gcggtgagcc ggtggcagaa ctgacgctgg
     2101 atcagaccat gccccggctg aaacggcagg taatcctttg cgaagcccgt cattgcggca
     2161 ccaggggtga tgaagacatt ttcacggtgt ttcaggacga aatcaaccgt gccggtctgg
     2221 atccggaaga aaacacactg attcatgtgg gggcactggg tttttgctcc attcaccgta
     2281 aaatgatctc cacctgtgtg atggttcgcc ctgagaatac ggtttatttt gtaaaagacc
     2341 ggaaagacgt acaggaaatt gttgcccagc atcttgtgga agggcagatt gtggatcggc
     2401 tgcgctgtca ggaagatccg atcagcctcc gcttctttga tctttacggg gatgcggctt
     2461 tctttaaccg tcagacgcgc atttcactgc gcaatgccgg tgtcatcaat cctgaaagtc
     2521 tatacgaata tatccattac aatggattca gggcgctgtc cctgagtctt tccaacgggg
     2581 accctgactg gcttgtggag cgtgtgctgg aatcgaaact gcgtggccga ggcggcggcg
     2641 gttatcctac agggcgcaag tggaaagatg cgcaccgcca tgcgaatgac cagcgctatc
     2701 tgatctgcaa cgccgatgag ggcgatcccg gtgcgttcat ggaccgcagc atgctggaat
     2761 cagatcctta cagtgtgatc gaaggtatga ttatcggcgg ctatgccatc ggagcgaccc
     2821 gcggcttctt ctatgtccgc gccgaatatc cgctggcgat caaacgcata gaaaaggcat
     2881 tggctgaatg ccgcaaacac ggccttcttg gtcagaatat tctgggaagc gatttttcca
     2941 tggatctgga agtgcgtctg ggtgccggcg cttttgtgtg cggcgaagaa acggcactga
     3001 tccactccat tgaaggagag cgcggccagc cccgcatccg cccgcccttc ccgacggaat
     3061 cgggcctctg gggcaagccg acggttatca acaacgtgga gactttcgcc aatattgcac
     3121 cgctgcttct ttacggaagc gactggtttg cttctgtcgg cacagagttc agcggcggca
     3181 cgaaagtgtt tgcgctggcg ggcaagatcc agcataccgg tcttgtggaa gtaccgatgg
     3241 gcacgacgct gcgcgaagtg gtctataaca tcggcggcgg tattcccggc ggcaaacagc
     3301 tgaaagccat tcagacgggc ggcccggcag gcggtgtgat tccggctgaa tatctggaca
     3361 tgcccattga ctttgatacg ctgggccggc tgggctccat catgggcagc ggcggcatga
     3421 tcgtgctgga cgaagacgat tgcatggtgg acgttgcgaa attcttcatg acattctgcc
     3481 agggagaaag ctgcggcaag tgcacgccct gccgtgaagg caccaagcgc atgcttgaga
     3541 tcctggagcg gatcaccatg ggcaaaggag agatggaaga tctggacaag ctggaacggc
     3601 ttgccagtct ggtaaaaaat acctccctct gcggactggg ccgtgcggca ccgaatccta
     3661 ttttgagcac cctgcgccat ttccgggatg aatatgaagc gcatgtgaaa gagaagcggt
     3721 gccccgcccg taaatgcaag acgctgattc attatgagat tgaccctgcg aaatgcgtcg
     3781 gctgcacgat gtgcgcccgc aactgcccga ccaactgcat cagcggcggc agaaaagagg
     3841 ttcatgtgat tgatcaggtg gaatgcatca agtgcggtcg ttgcttcgat gtctgccgtt
     3901 ttgatgcggt aaacaggaaa taaccgggcc gtggaattcc ctccacgctc ctgacatata
     3961 aaggtacagc aatgagccag gaaaacatgg ttactcttac aatagacggc gtcaaagtga
     4021 cagtgccgga cacaatgacc attatggaag cggcggaaac gcagctgggc atccgaattc
     4081 cccgcctgtg ctaccacccc gacctgagca tacaaggctc ctgccgcgtg tgtatcgtgg
     4141 acgtgaaagg tattcccttc tatcaggcgt cctgtgcggt aaaagtggcg cagggcatgg
     4201 aagtggagac caataatccc gccatccgcc aggcgcgccg cgacattgtg gaactgcttc
     4261 tggacaacca cccgcgcgcc tgcctgacct gtgaccggga cggcaactgc gaattgcaga
     4321 atgtggcata tgcgacaggc gttcgcgaac gtctttttgc gggagaacgc aagcgcttcc
     4381 ccattgaaaa cacgaacctc gctgtagacc gtgatccgga aaaatgcatc ctctgcgggc
     4441 gatgcgtccg tatctgtgac gaagtgcagg gcgtgcacaa tctgagccag cagggccgtg
     4501 gcttccacac attagtggcg cccgctcatg atgcctgcat ggatgactct gtatgcatcc
     4561 agtgcggtca gtgcgtggca gcatgcccga ctgccgcttt tacagaacac gaccacacgg
     4621 aagaagtgtg gaaagcgctg gcggatccga ccaaacacgt tgttgcccat acggcacctg
     4681 ccatccgcgc caccatcggt gaaggtttcg gtatgccgct gggaacgcct gcagccggaa
     4741 aactcgtaac agcactgcgt cgcctcggct ttgaccgtgt cttcgacacg aacttttccg
     4801 ccgacctcac gattatcgaa gaagcgaacg agttcatgca gcgtttttct aaaaacgaac
     4861 ggctgccgct gctgaccagc tgctcaccgg gctgggtcaa ttttcttgag aaattctatc
     4921 cggaactgat tcccaatgct tcgacctgcc gctcccccat ggcgatgatg tcttcgctgc
     4981 tgaagaccta ttatgcggaa aaaacgggca ttgatcccaa ggatattttc gtagttgcga
     5041 tcatgccgtg cacggcaaag aaattcgagt gcaaccggcc ggaactgtgc atgcccgacg
     5101 gcacccgtga aaccgatgcg gtgctgacga cgcgcgaaac catctggatg atcaagaact
     5161 acggcattga cctgcgccat ctgcccgacg gtgaatttga ttcgccactg ggtgaatcct
     5221 caggcggcgc cgatattttc ggcaccaccg gcggtgtgat ggaagcagcg ctccgtgtcg
     5281 cagtggaaaa ggtgaccggc caggcgcctg aaaacatgga attcacggaa gtgcgtgccg
     5341 tggaagggtt gcgcgaacgg gaactgaaag tgaatgacga gctgaccctc cgggtgggtg
     5401 tcgccaacgg gctgaccaat gccaagcagc ttctggatgc ggtggtgaag ggtgaaaagg
     5461 agtaccacat catcgagatc atggcctgcc cgggcggctg tataggcggc ggcggccagc
     5521 cctacccgcc tgaaggctac cggattctgg ataaacggct tcttgcgaaa cgcgcagagg
     5581 cattgtataa aatcgatcag ggcaagaaag tgcgcagttc cgggcacaac cctgccatcc
     5641 tgaaccttta tgaagaatac ctcggagaac cgggcggtga aaaggcgcac cgcctgttac
     5701 atacccacta tgcgccgaga ctgcccagag gaatacgcta atggacgtta aagtaaccga
     5761 taacagagac gaagtgttca gcaaaagcaa agaagcactg actccggaaa ttgttgcctt
     5821 tatcgaagac tgcacgcaga agcagcatcc gaacagccat ctgatcaatg tgcttcacaa
     5881 ggtacagggt catttcggct atctgggaaa agaacagttg gatgcggtag cgcagctgct
     5941 gcaggtgccg acagcgaaag tgacaggggt tgccaccttc tatcacttct tccgcctgga
     6001 gccgcgcggc cgtttcgtga tcaatatctg tctgggtacg gcatgctatg tgaaaggtgc
     6061 tgagcgtgta tgcgaacgcc tctgcgacga gctgggcatt catgtgggcg agacaacacc
     6121 ggacggcatg ttctcccttg aaggcacacg ctgcgtgggc acctgcgggc tggcgcctgt
     6181 agtgctggtg aacgacacgg tcttcggcga agtgactccg gatcagattc cggcgcttat
     6241 cgaatcgcac attaaaaaag cgcaggcaat ggaaaaggaa agcgtggtct gacgaaggca
     6301 gtgcccttcc ccctcccctt ttctactcat agggctggac ttcatcgaag aggtattcga
     6361 gactgatgcc ggcggtgcgg aacatttcct ccgattccgc accggcatgg taacgccttt
     6421 cacagacaac ccggacaatg ccgcagttga tgagcagcat ggcacatacc cggcacgggg
     6481 tcatacggca ataaagcgtg gcgccctgaa gggatacgcc gagccgtgcc gcctgacaga
     6541 tggcattctg ctcggcatgg acggtgcgca cgcaatgctc cgtgatactt ccgtcctcgt
     6601 ggcgtacctg ctttatctgg tgaccgacat cgtcgcaatg gggaaagccg gcaggagaac
     6661 cgacatagcc cgtgacgagc agggtacggt cgcgggcaat gacacacccg ctgcggccac
     6721 ggttgcaggt ggcacgtttt gagatggcat tcgccacctc catgaagtat tcatcccagc
     6781 tggggcgtac atgctccgga ctcacgggca cctcctcctc ttccatgcct taaaagggct
     6841 cgtccagttt ggttacgggg tcacaggtaa gaatacaggc gctgtcaggg tgctgtcgga
     6901 taacgagacg atcgccttcc tccaggtcca caaattcggg cgaattgtcg aaggcgagcg
     6961 tagccggacc ccgtgtaatg acaaagtgca gctgactgtc ttccggcagc acaatatgat
     7021 tcaccgcatg ggtggtcgcc ttgaaagcca cgcctatacc ttctgtaaaa atccctttgg
     7081 tgattgcctt gaagtaggca ttgctgccga aagcggtaca gaccacaaaa ccgtcgctca
     7141 caatttccac gccgtcttca aaaggtacgg cattgacgga aagctgaaac cggacggcgc
     7201 tgttgatcct gcccatatgc acattcactt cgttcagaca ggtgaaggaa aaatcaggtg
     7261 ctttcacgcc ttcgcccgta aaaatactgc attcaagctt ggtgtacagt gtccggacca
     7321 tgtcacggcc ggaaagggcg gcaatcactt cttccgggcg gcgctgaatg cagcggttcc
     7381 cccgtttgct gttgaggatg ggcaccttgg ggataccggg ccagtgcagc tccgccgcca
     7441 gcagtgtacc gtcgccgccg taacagatca ccagatcagg cgttgtatca acaacgacaa
     7501 gatccgtttt ttcctgaagc agcggtacga gcgaggatgc ctccttcccg aaaagcgcca
     7561 cgcgaatctg ttctttttcc agaggacttt gaaatgctgt ggaaaccatg gagcggatct
     7621 ctttttgact ggaccgaccc ggtgcaggtc aggacgcttc cttttcagtt gcggattttc
     7681 gacggaaggt cagccagagg ctgaaggcaa aataataaat caggtacagc atggatagca
     7741 gaatgagcgg atggtgccct ttcgggtaca ctgccagcgc tcccagcact gcagccgccc
     7801 acagcaaacc gagaagaata ttaatggcac ggtaatgggg cgtgcggctg gggtaaagat
     7861 agcggacagg aataaagacc gctccggcac agagcagcag aacggccgca ttgacgactg
     7921 gcggccattc cagaaggaag aggtagcaga cggcgatatt ccaataggaa gggaaacccg
     7981 taaaggcatg atcggcagtc tttgcctctg tctgactgaa ctgataggca gaagaaaaaa
     8041 taatggcggc ggcaacaggg agacgccagg cttcggtgag gacaggcgca taaagaagaa
     8101 aataggcggg aaccagcaca taggtgaaaa aatcgaccat attgtccagc aatgccccgt
     8161 caaaataggg cagatgaacg ctgacgcgcc gccaccgtgc cagagcgcca tccacgccgt
     8221 caatggcgat ggtaactgcc atccagccga gggcgataac aaaggctccc cgggcgctcg
     8281 cctgcagcgc cagcagaccg aagagagcac ccgatgctgt gagaatgtgt accagccagg
     8341 cacccggaat gtcactaatg ctgcttttcg aggtcattac ccttttcctg acacggaaat
     8401 tcgctttcgt caaaacatac tgaagaaaga gggaatcagg cagaaggcac ccatcccctg
     8461 attgattatc agcctgtact atacaacagt atccgttgtc attacgaatt tatcagaggg
     8521 gcgcccggta ctgctgcagc gtgcacttcc ggatgcgcgc tgccaagccc gcacagggga
     8581 ttccatgatg aatcgggggg catctgtgtt ctatactttt cggcatcagt tgcaacagca
     8641 ttcaaacgaa tgaggtacag agcaccatga caacactggt ctggctggat tggctggtta
     8701 tcggcggcta ttttgccgca gtagcggcgg tggtgtggtt cacggcacgt cagcagtcaa
     8761 cctcggagga ttattttctt gcgggaagga atatcggctg gtttgccatc ggcgcctcgc
     8821 ttttcgcctc caatatcggt tcggagcatc tggtggggct tgccggtgcc ggtgcaaaaa
     8881 gcggcgtcgc catggcacac tacgaactgc atgcgtggtg tctgcttatg ctgggctggg
     8941 tgctggttcc tttttattac cgtgccggga tctataccgt gcctgagttt ctggagcgcc
     9001 gttttaatcc cgcctgtcgc tggattctgt cgctcgtaag cctgatcgcc tatgtcttca
     9061 ccaaagtgag cgttacggtt tatgcgggcg gcatggtctt tcagacactg ataccggagc
     9121 tgcagtttgc tgggctgaac agcttctgga tcggtgcggt aagcgtggtg ctgctcacgg
     9181 gcgtatacac gatggtaggc ggtctgcgtg cggtggtgta taccgatgcg atgcagaccg
     9241 tggtgctgct gctggggtcc ctgtgcatca cgctgatcgg gctctaccat ctgggcggct
     9301 ggagtgtact gcgcgagatg aacggcagcg tgcgctttaa tctgtggcgt cccgcctccg
     9361 acccggaatt tccctggccg ggcatgcttt ttgccgcgcc tattgtgggg ctgtggtact
     9421 ggggtgcgga tcagtatatc gtgcagcgga cgcttgcggc acgtagtctg aaaattgcac
     9481 ggcgcggcac tattttcggc gcttatctga agatcacgcc tgtttttctt ttcattgtac
     9541 cgggtatgat cgcttatgca ctggttcaga agggcatgct cgtgatggac agccccgatc
     9601 aggcatttgc cgcactggtc acacagcttt tgcctcaagg cgtacggggg cttgtagtag
     9661 gcgggcttct tgccgcgctc atgagttctc tttcttctct cttcaattcc tgctcgaccc
     9721 tcttcacggt ggatatctat aaaaagctga agcctgcggc gcctgaatcg gaactggtgc
     9781 gggtaggtcg cattgccacc ggtgttgtag tcgggctggg tctgctctgg atccctgcca
     9841 tgcagctggt gtcaggtgcg ctctacgaat acctgcagag cgtacaggcc tatctggcac
     9901 cgccgatgac ggctgttttt tttctgggca tattctggaa gcggatcaac ggtaccggag
     9961 cggtggtcac gctggtatca ggatttgtcc tgggattggc aaagcttggt gcgcagattt
    10021 acgcaggcat cgccgtatct gatatgatta tgcagaagcc ttggatcctg cggctgatca
    10081 tcgcatacgg gaatattaat tttctgctgt tttgcgtgta cctcttcctc tactgctgca
    10141 ctgtgctggt tctggtgagc ctgctcagcg cagcgccaac ggcggcacag accaccaatt
    10201 tgtgctattc cggcaatacg gaagcgggcc gccgggaagt gcgggaaagc tggaattact
    10261 gggatgtgat tcacaccgct gccgtgctgc tgctgattct gagcgtctat ttgtatttca
    10321 ccggatgatg cctgagcact ctccgattgt cttgaaaaca ggagcctgaa ccatgggtcg
    10381 ttttatcgca aaacattccc ctcttttcat tctcgccgct ttgtgcatca ttctggcggt
    10441 ggtctcccct gtcttccgct caccctcaaa tctgcaggga gtggcgcagc gcaccgctgt
    10501 tgtaggcatt atggcggtgg gtcagctggc ggtgatcctg accgccggta tcgatctttc
    10561 ggtaggcagt attgcggcac tgagcggtgt ggtgggctgc ctcgccatgg taaatctgtc
    10621 tgtccctgcc ataccgggta tttttatcgg tatcggtgtg gggcttttgt gcgggcttgt
    10681 caacgggctg ctgacggcga aagggaagat tccgcccttt atcgtgacgc tgggcatgat
    10741 gatggcggca cgaggcgctg ctttgacagc atcaggcgct acaccggtct tcggattgcc
    10801 ggaaaacttc gcatggctcg ggggcacccg gggctggtgg cttcctgtcg tttttatgtt
    10861 cggaatcgcc gctttttttg ctgtcgtact cggatacacc cgattcggca gagcgcttta
    10921 tgtcatcggc ggaaacaagg aagcggcgcg cctctccggc gtaccggtag actgggtacg
    10981 catcgccgcc tttgccatca gcggtacagc ggcaggcttt gcaggcatga tgctcgcttc
    11041 ccgcaccagt gttgcttcgc cgacagcggc ggaaatgtat gaacttcagg ctattgccgc
    11101 ctgtgtgatc ggcggagcca gcctcatggg cggagaaggc ggcgcttttg cgaccatcgc
    11161 cggagcactg atcatgacgg tgctctataa cttctgtaac ctgcagaaca tcaatgtaca
    11221 ttggcagcag attctggtgg gcgtgcttct cgtggcgctc gtattttacg acaaccgccg
    11281 aaaacgcaaa gccgggcttc ttaaagactg aagcgcacgc tgtttctgaa tccatcagga
    11341 gagtcccatg aaaatacagg tgatgggcgc cggcggctgg ggtattgcgc tggcacgtca
    11401 tgcgtgtcgc ctcggccatg aaaccgtact gtggggcagg aatccggaga agatgcagcg
    11461 acttgcccgg gaacgtcagt cgccggagct gctgcccggc atcctgcttc cgccggaagt
    11521 gcgtgtcgtc actgaacccg aaaaaacagc ggatctggtg ctctatgccg tgccgtccaa
    11581 aggcatgaaa gatacgctgg aacaataccc ctttcccctg tctgtaccgc gtatcagtgc
    11641 ggcaaaaggc atagagcagg acagcctgct gcggatgagt gagcgcatag ctttaagtgc
    11701 gccgggcgca ccggtagttg tattgtcggg accgagtcat gcggaagaag tgggtcgcga
    11761 tctgccgacc tgtattgtgg tggcgggcac agatgtcgct ctgctgcagc aggtgcagca
    11821 gtcactgggt gctcctcatt tccgggtcta cacaagccct gatgtcatcg gtgtggaact
    11881 ggggggcgct ttgaaaaata tcattgcgct ggcggctggt gcgtgcgaag gcttcgggct
    11941 gggcgacaat gcccgggcag ccctgattac ccggggcctg gcagaaatgc ggcgacttgg
    12001 tgtagcatgc ggcgcagagg cgtccacctt tgcaggactg ggcgggctgg gcgacctcgt
    12061 ggtcacctgc gccagtcatc attcccggaa ttatcgggtg gggcttcaga tagcggcagg
    12121 caggacgctg gagcagatac aggcagacag cgtccaggtg gcggaaggga tagaaactgc
    12181 gcggagtgcc tggcgtcttg cccggcggca tgctgtggaa atgcctgtgg tgaaggcagt
    12241 gcatgacacg ctttttgaag ggctggacgc ccgcagcgcc attaccgggc tgatgacccg
    12301 gctgatgaaa gcggaatggg aaagttgact tccctaggct caagggatac aatttagtca
    12361 atagaggtat gtctttccgg tgaacctgct tttgtgtgaa agagtgaaac cggagaaaga
    12421 atggctggtt tatgtaccgc gtcgggaagt actgctgctt ttttaacagt gcgcttatat
    12481 cgggcaattg attttgcgcg cagataaaca gggagatttt ctcatgcctt ataagcagat
    12541 tctgatgatt ctggttctta tgctgacctc ttttaccgtg ctggcacagc caccggaagc
    12601 aggcggtgac gatgccgccc tgctggatcc ctattcgaat ccgccctccg gaataaaaga
    12661 agagaaatcc cctgaactcg aaggcgatac tattgtcttc aaaagtggca gtgagctgaa
    12721 gggtattcat attattcggg aatcggcgct tttcgttgaa gtggaatacc tgccgggaga
    12781 gtcgcccgta aaattcccca catcccagat caaggaaatc attcgtcagg accctgccag
    12841 ccatgccagc ggcaatgccg gcgccgccgg cagcaagccc gatctgatgc cgggagaaga
    12901 agtctcagcg caatttcatc aggcacttat gaccgtgctg agcgagacac cgttggaact
    12961 gaccaacgcc gattatctcg ccgttattaa agactttgcg gaaaaagcgg aagccccgct
    13021 ggaaatcaaa gagcgcgtga ctgccatccc cgagcaggag cgtgttttta cgcgcaccct
    13081 ccctgcaggt attacgctgc tcgattttgt gcagaaagaa ttttccgaaa ttgcaccgaa
    13141 agtgcgtgcc gtgctgcagt ttgacaagct cattctggaa cttcgggaag cccctgtcga
    13201 ataaattccg cctgccattt ttttccctta taagcaatca ccgggaatgc tcttcactgt
    13261 ggcgttcccg gtgatcagta tctgttgagg aatcaccatg tcacagccct gcatatatcc
    13321 tgtggaactg atgcaccggc ccgcttccct gcttcgtatc cgtccgaaaa cacggggact
    13381 gtatcatcag cgcatgaatg tggtttatgc ggaggtgcac ggcaccgggc tgcttatgga
    13441 tattttcgca ccttcaggaa tatcccgggg catcggtgtg gtggatgtga tcagcggcgg
    13501 ctggcacagt gaccgcgcca tgctgcacca gcacataggg cttgggctca ttgacgcact
    13561 ctgcgaacgg ggctttaccg tttttgctgt atcgccggga tcggtgccgc tttttaccgg
    13621 acaggacatg gtcaggcata ttcatgcggc aatccgccat ataagacagc atgccgtcac
    13681 ttatggtatt gaggaaacac cgctgggcat caccggtgta tcagccggag ggcatctcgg
    13741 cgcgatggcg gcactgaccc cgcagaaagc gcgtcccgat gcccgcgagc cctggcgaca
    13801 gcagagcacc gacatccagg cggcagcttg ctttttcttt ccgaccgacc tcgtggacta
    13861 cggcggtcag ggctttgacc ggctgcagat ggatgggttt aatccgctgg agctgctttt
    13921 cgcaaaacat gaaaaagctt cgatagacac tgaattgaag ctgcgcctgc aggcactttc
    13981 cccggcgttg ctccccattg tctcgccgcc tccctttctg ctgatccacg gaaaacagga
    14041 catgctggtg ccctgggaac agtcggaaaa actggcttcg gcgctccggg cagccggagg
    14101 agaggccgcc ctcctctacc gggaagaggg tggtcatttc tggccggata tttcagtgga
    14161 ggtcgatgcg gcggctgact ggctggagca acgcctgacc tgatacctat tcggcagatg
    14221 gcggcccgcc taaaagctgt ttcactgcgc ccatcagcac tccttccact tcaggtgcca
    14281 cattgctcgc cgcttcactg gtttcatagc cgccctgctc ataggcaacg gctgtaccga
    14341 tgtagcccgg gctgtagtcg ccataggccg ccatggcgac aaaaagatcg gggcgctgct
    14401 ttttctccga cagctgatat tccacgaaaa gttcgccggg catgtgcaga atccgggcag
    14461 tacccagact cagacagggg atatcaaact gatgtcccga ttcctgccgt tcaagccagg
    14521 caagtttgct tgcactgccc agaatacggg caggatccgg ctgttcctgg aatcccgcct
    14581 ctatgccttc tctggagagc cagggcccgg tgggcagggt gaccagcacc gaattccagc
    14641 caacggcatc cgccgtcagg ggatacttct ccgtggcgtc ccaggcgcgt ttcataccgt
    14701 ccgccagccg tttcgccagg atcagccggt tttctttcga tccgtcattg tattttcctg
    14761 cgccgatatt ggagcctgcc ccgttaaaat gcacatggag tgcggagggc acctcgagct
    14821 gacggagaaa gcgggcaacg ccggggaaat ccgggttggg aatgccggtg cggtaatagc
    14881 tctgcgggtg ggtcgcataa tagctgagca cagcaagggg cgtatcttca ttccaaaaac
    14941 tcaccagcga tacttcaggg tcgatgaccc cttcgggctc ggcacggaca gcaggatcgg
    15001 cgcaggcagt gaagcgggta acccgtactt tgccgttctc atcaagaata cgccggttgg
    15061 aggcaacttc atacacttca gccttgccca ggccgaaatg cgtcacaggc tccgttttct
    15121 ccattgcccg caaaacagtg ctttccagtt ccgcgagaac ctgccgctga aaggtgcttt
    15181 catagcgttt aggattagct ccgagggaga gcgcaatccg ttctgtcgtg aaatcacagc
    15241 cgggagcatc gtgctggtgc agtgtatgta ccgccactct ttccggctgt gtccctgcgg
    15301 cacgggcgag cacttccctg aaagcatcgt gactttcatt gccgatgcct atccagtcaa
    15361 cagcacacag cacaatggga tcaccggcgc cgcaaaggac aattccacgg gcgcgcagac
    15421 tcatgtccca ggtacctgca acggagtcat aggcaagatc cgtaccgaca ggaggggtca
    15481 catcgagatc aaaaacggcg attttcatgc tgccctctga aggtgctgcc ggacagggaa
    15541 atccagccgc aacaaacaga agcagcatgg gtatcaagac ggtataaata atctgttttt
    15601 tcataaacgg gtctccttga ccatctggtc ttcaaaacat tttatcagta tactacagaa
    15661 aaagaggacg catcattccc cttcatcttt tttcatatgg tatgataggg gtcgtatttt
    15721 acagtatggg gcagtcattc aacttaccgg cagcagcaat tccggcagaa taatatggag
    15781 tcaagatgag taaaggcatc accaaacggt ctgaagatta ttcacaatgg tatctggatg
    15841 ttgttttgaa agcggaaatg gcggactatt cgcctgtgcg cggatgcatg gtcatccgcc
    15901 cgacgggcta cggcatctgg gaaaaaatcc agcagcagct ggaccgcatg ttcaaggaaa
    15961 ccggacatgt caatgcctat tttcccatgt tcattccgga aagctttctg aaaaaagaag
    16021 cggagcacgt ggaaggcttt gcaccggaat gcgccatggt cacccacggc ggcggcaagc
    16081 ttctggaaga accgctggcg atccgcccca cctcggaaac cattatctgg tccacctata
    16141 aaaactggat cagctcgcac cgtgacctgc cgctgctcat caatcagtgg gcaaacgtct
    16201 gccgctggga gatgcgaacc cgtctcttcc tccgcactac ggaatttctc tggcaggaag
    16261 gacatacggc tcacgccacg gaagaggaag cggaagaaga agccttcaaa atggtgcatg
    16321 tctaccggga ttttgcagag aactacctcg ccatgcccgt aatggtgggc cggaaaacag
    16381 aagcggagaa atttgccggt gcggaacaca cgtactgcat tgaagcgatg atgcaggacg
    16441 ggaaagcgct gcaggcgggc acttcccatc atctgggtca gaaatttgcc caggcctttg
    16501 atgtgaagtt tcaggacaag gacggcgagc tgaaatatgt gtatgcttcg agctggggcg
    16561 tgtcaacccg gcttgttggc ggtatgatca tgacccacag cgatgacagc gggcttgtgg
    16621 ttccgccgcg gctcgcaccg acgccggtgg tttttgtgcc gatctaccgc aaagacgaag
    16681 agcgggcgct ggttctggaa aaagccgaag cactctgcgc cggatgggac aaactctttt
    16741 ataaaattga tgaccgggat cagtacaagc cgggctataa atttgctgaa tgggaactga
    16801 aaggggtgcc cgtccgtatc gaaatcgggc cccgggatgt cgcttccaat tctgtggtgg
    16861 ttgtgcgacg ggatacaggc gccaaagaaa ctgtatccgc cgacacgctg cacagttatg
    16921 tggagacgct gctcaatacc atgcagaccg acatgtacaa tacagcgaaa aagcgcatgg
    16981 aagacaatac acacaccgtg gacagctacg acgaatacaa ggagcgtgta ggcggcggcg
    17041 ggttttatcg tgtctttctg gatcacagca accctgaagt ggaaaaacag atgcaggaag
    17101 atacccggtc caccatccgc tgtatcccct ttgatgcgcc cgaagaacag ggcagctgca
    17161 tgatcacggg agcaccctgc tccagccggg tgattgccgc ccagtcctac tgatacagcc
    17221 gtaaggaaag aaaacaaatg acccatgtca atctggtaac aggcggcgcc ggctttatcg
    17281 gctctcatct ttgtgaggcg cttctggaac gtggtgaaga agtgctttgc ctcgataatt
    17341 tctttaccgg gcgcaaaacc aatatcgaac atctgctggg tcacccccgt tttgaactcg
    17401 tgcgacacga cctggtgaat cctatcgtgc tggaaacgga tcgtattttt aatttcgcct
    17461 gccctgcctc gccggtgcat taccagttca atccggtcaa gacaatcaag accaacgtca
    17521 tgggcgcgtt aaatatgctg gggctggcaa aacgcgtagg cgcccggatc ctgcaggctt
    17581 ccacgtccga agtttacggc gatcctacgg tgcacccgca gacagaagat tactggggca
    17641 acgtgaatcc tatcggaccg cgcagctgct atgatgaagg caagcgtgtg gcggaaacgc
    17701 tctttttcga ttatcacaac cagaatcagg tggacatccg ggtcatcagg atcttcaata
    17761 cttacggacc gcgcatgctc gtcaatgacg ggcgcgttgt cagcaacttc attgttcagg
    17821 cactgcgcaa cgcacccatc agcatttacg gcgacggcgg acaaacacgc tctttctgct
    17881 atgtaagcga tctggtccgg ggcattctgc gtatgatgga tacagacgga tttcacggtc
    17941 cggtcaatct gggcaatccg ggcgaattca ccatcctcga gctggcgcag aaagtgatcg
    18001 acctcaccgg ctcccgatca gtactggaat accatcctct tcccgagaat gatccgaccc
    18061 ggcggcgtcc cgacatcagt ctggcggaag aacgcctcgg ctggtctccg aaagtgccgc
    18121 tggaggaagg gctgacagag acgatccgct atttcgacac gctgcttcgg gagggatgag
    18181 cccctccctg accgcggcgg caataccttc agaggtaaaa tactcaggca tatgcagcca
    18241 ggcatagacc gtgagtttcc ggattgcccg taccagccgc cgatgtaccg tagtgctgtg
    18301 gcaccgcaga tgccgggcac attgccgtgt tgtatacccc ttaaaataat aaaggacaat
    18361 ggcgcgggat tcggcagaag aaagacaccg ggcaacggca aaacgcagcc attccagttt
    18421 gacagcgcgc tcacgggcac gccgctcagc ttccgcctgt tccggcgccg tctcatacca
    18481 aagagagcgg ttatcgggca gcgattcaaa tacctgaggt tggacactct tttcccgccg
    18541 atggaaatcc acaaattttc ctgccatagg aacgctcctg aattgaagtg atcggatcag
    18601 gggcgcacag tgcgcccgtc actgtgcaag catagtcagg agaggcggaa aaagactttt
    18661 gccttttctt gttcttttat ttgttttttc tttggggcgg aaaagacggt cagtcaggat
    18721 tgaggcggct gtagtccacg gtatggtaaa cgcctttttc aagatggcgc gccagaatga
    18781 agagaaaatc gctcatccgg tttgcaaagg caagcgggag tggcgcatta aaccccggag
    18841 cgacacgctg atagccgaca agcctgcgtt caaaactccg cgcggcggca acggcaagat
    18901 ccgccagtgc tgcatcttca ttggaagcgg cggcgataaa agcgccgggc aggttgagtt
    18961 ccctttcgag ccggtgctgc tcctgctcaa ggcgttcaag atgctgcggt gtcagggcgt
    19021 gaggtgattc gggatcggag gattccggat cactgataaa agcgccgagg gcgaaacatg
    19081 cgtgaagcag aaaaaaaaga cagtcggtac tttccttttc ttccgcaatc ggctgcagtc
    19141 gtatctgaag cagcgccaga tgggcgcgca atgtttccag agcgcccagc gcttccatca
    19201 tggcatgatc cttgggcagc gtgctcccgt tcagtgcccg gctttccccc tgatcccctt
    19261 ttcctgttgt aatggaacgg tgttgcattt ccttttcctt gcatggtgcg cgtgcagttt
    19321 tttctgttca gtcgtcttcc ctgtcctgct tccagaagtc cgggtcttcc acgacttcgt
    19381 tccaggtgaa aacaggcgga tcgcggtcta ccagcgcctc aagttcttcc tcttcccatt
    19441 ttcggtagag tcctttggac agacggcggt gccgcagata ggcaagaatc agcagaatgg
    19501 caccgggctg aaaagccgca cccgatgctg tcagtgcaat aatcgtatat ttccagaggc
    19561 ttttttcata accccggcag aaggtttcaa gatcgatacc gccctcactg atcagcgcct
    19621 tgccgaaggt ctgctccttt aaagcatgaa tgactttcca gaacttctct tcgcccaatt
    19681 gccggtgcaa ctgacgggtc atgtaaagcg cctgaacata ggcatcgctg aactcttctc
    19741 ttcccagagg ctggtaaaaa gcgttgtcca gttcatgata aggaataatt ctgttgcgta
    19801 caaacatgac cgctatcctc atggcgccgt cccagtagtg ctgattggaa aggtacatgg
    19861 caagcccttc gttcagccac tggggcaggc ggtcataacc gatgctccgg aacagcagca
    19921 ggtgcaccag ctcatgacgc agtgttcccg catagtcgcc gccaggctcc cggaggcggg
    19981 gtgccttcac ggcaataagc ccggctcccg gatgcgccac accgctcaca tccagcccgg
    20041 caaaatgtac ggcatactga tcaaaatcat caagcgtatc cgcaatggca acatgcacag
    20101 gggcttcccc tgccggcaga taagcgctga attctttcag cgcctcttcc agtatttgaa
    20161 gagacagtac tgccgtttta tgttgcgacg gaacataatc aatggtaaaa attccctgct
    20221 ggagccgttc tttttccggc agaggcggca tctgagcagc gcagtgcaaa gacaccgccc
    20281 aaagaatcag aaagagcagc gaagggacaa atgaagaaaa ggtgtgtttc atgtgtttat
    20341 tatacgcagg ccggccgtcg aaaagatacc gggcagcatt tttacgaaaa tcgactgtca
    20401 caaaagaaat agagcgcttg cttttcccga ataacagagc atccgggcat ttttaaaact
    20461 tcaggcgggc ttttttatca cctattttgt ataataggcg acgattggca gaactgtatc
    20521 aacaaaagga ttttctcgtt ttatgatcga aattctttcc aagccttcaa tgctttctgt
    20581 tgttgtactt ggcgcatcgg gtgatctggc agtcaaaaag atttttcccg ccctcttctc
    20641 tctttattgc cgcaggctgc tgcccgcttc gtttcactgc tacggctttg cccggacgga
    20701 aatgagcgaa agtgccttcc gccaactgct gatgagtcat ctcacttgcc gctatacgcc
    20761 gggagagaca gaatgccatg accttatgga gggctttctc gggcattgca gctacgttca
    20821 gggtaactac gatgaccaag cctcctttgt ccggctgcag cgggagcttg aaaaacggga
    20881 gcataaagac ggcacaacca aacgtatttt ttacctgtca gtgccgccct tcatttttct
    20941 gcccgcatcg aaaacgcttc atgagaccgg tcttctggca gaaattccgg gaaccctgca
    21001 atgccgtctt gtactcgaaa aacctttcgg cagagaccgc gcttcctcgg atgcgctgac
    21061 aaaggacata gcagctcttt gcgatgagtc gcagatatac cgcatcgacc attatctggg
    21121 caagg
//
LOCUS       DAOPWB010000049        20936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2530_length_20936_cov_5.517791, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000049 DAOPWB010000000
VERSION     DAOPWB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 20936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20936
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2530_length_20936_cov_5.517791"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /gene="acs"
                     /locus_tag="PKN92_06330"
     CDS             complement(<1..2021)
                     /gene="acs"
                     /locus_tag="PKN92_06330"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNZ48446.1"
                     /translation="MGTEEKKSLSSLLTENRTFPAPEEIRANALVKSEAEYQAMWQAS
                     VNDPDTFWLEHAKKELSWFKEPTKSLEYIWDTERRLIEHTWFKDGELNVSYNCLDRHL
                     GTEREDKIALIWQGEPEDDVREITYKELHYEVCKFANVLKGLGVKKGDRVCIYLPMIL
                     ELPIVMLACTRIGAIHSIVFGGFSADAIATRVGDASCKLLITSNVSLRSGKHIGLKAI
                     ADEAMAKPDCKTIEHCVVVKRNAEPCSMQEGRDLWYDDLMEEASEVCEPVALNAEDPL
                     FILYTSGSTGKPKGVVHTTGGFLLQVAMTHKYVFDLREDDIYWCTADIGWVTGHSYIV
                     YGPLANGATSVMFEGVPTYPDPGRFWHIVDKFQCTQIYTAPTAIRALIQKGDEWVNKY
                     DLSSLRILGSVGEPINPEAWMWYYTVVGKSRCPIMDTWWQTETGGFMIQGFPCFTLKP
                     GSANRPFFGVDPVILRDDRTEVGLEEGGKLCIRKPWPGMMRTMWGDHDRFIDTYFTMF
                     SDLYFSGDGCRKDADGDYWLMGRIDDVVNVSGHRIGTAEVESALVSHPKVAEAAVAPM
                     PHDIKGQALYAYVTLRENVEESEELRKELVKHVRTEIGPIAAPDVIQFAPALPKTRSG
                     KIMRRILRKIAENATDQIGDTTTLADPSVVDALVAEHKRLIGGQSAVPVS"
     gene            complement(2371..4407)
                     /locus_tag="PKN92_06335"
     CDS             complement(2371..4407)
                     /locus_tag="PKN92_06335"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNZ48447.1"
                     /translation="MAVSSSFRSGIVLLLICFLVSVSSGALDIVMPAGTPPRLFMTED
                     EMMRLRARAKDPQWEKQCAGLLKEADSCLENVPAIPHQGGQWTHWYSCPKDGGSFITD
                     TPTAHRCSVCGEVYSGYPYDDAYITYMHNRYNNNLQTIAIAYVIEPTGVRAKHIRDSL
                     LEYASFYEDLPLHDRDGNTTLSAARLYAQTLDESVNLCKFCFAYDLVYGDPCFSEEDH
                     RIIQEHLLLPMCETIQRYDMRISNWQTWHNSAVGCAGLLTGERELVDWAINGKSGLMF
                     QMERSVLSGGMWYEGAPGYHWYSFHALYYLLEAAGRAGMNLWNLPPVKRMLDGPLRQL
                     YPDLTFPAIHDSPRTSILGNRGFYEMGYSHFKDERYLPLLTVRDSRDALLYGADSLPE
                     EKGALTLDSYNDDEEALAVLRNPDGKTAVFLDYTRARVGHMHPATVNLLLFAQGEERL
                     AEAGRLPYGNPLHKEWCRQTVSHNTVVVNETSHKRIESTCTFFEVGEGWRAARAMATL
                     DGEKYALNRTVVLADKAVIDIFRCRSKEDALFDLPLHFRGEWADLPEMKEEAVSFEAP
                     GYREFREVKEAVSHADWYTVATGASSRIVLQIADAGQQFVGRSPGYQYEENLPMLMRR
                     LSGKEAVFVTVFHLLEGDAVPDAARCSTDASGTVTVQTGGLTVQADENALRITP"
     gene            complement(4472..4954)
                     /locus_tag="PKN92_06340"
     CDS             complement(4472..4954)
                     /locus_tag="PKN92_06340"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNZ48448.1"
                     /translation="MGIVRKPKLTEIADMKALLDEAAAARQVLPRELSEMLENARDFF
                     VYVDEEGVGGMAALHIDLVDLAEVRSLVVRDRLRGRGIGSMLVEATVEEARQLQIARV
                     YAFTRQPEFFKRLGFEVVDRAELPYKALKDCQRCPFFAQCDETALVRPVPPAEMTGVA
                     "
     gene            complement(4988..6097)
                     /locus_tag="PKN92_06345"
     CDS             complement(4988..6097)
                     /locus_tag="PKN92_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNZ48449.1"
                     /translation="MAFFKNIPFGADSCGMVHYCGMRRGAYERVKAVMAEFFDQETGL
                     ILSVLREAAMLAVAVREESALFTVEKSDLSPVTVADFAIQALVSRRLGDVFPDAVLVA
                     EEDASSLRAPDGCDMLEVITSFVSRFIPGATGGAVCDWIDQGAASEGGALFWTLDPID
                     GTKGYRRGGQYAVALAQIEDGHVIRGGLACPALGAGELPAFAGTGLVAAAKRGAGAWL
                     FPISSAAGPSSAAALHVNGCSDPSKARMLRSFEAGHTDVSALEQLADALNLRPEANVL
                     MDSQAKFAVLASGGAEFIFRLLSPERPGYKEYIWDQAAGSLVVEEAGGRVTDLKGDRL
                     DFSQGRRLLKNTGVVASNGVLHDAALAALQMLPSL"
     gene            6150..6725
                     /locus_tag="PKN92_06350"
     CDS             6150..6725
                     /locus_tag="PKN92_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48450.1"
                     /translation="MPCDHCKKDKELKSYAEVKDGQIYRLSLCEECLALYLSKAPEFS
                     RNAFSRTAAPKKAKVRREDSPTGLKATPEKEATSRPPDIPETCPDCETSWLQAKKGRP
                     GCPGCFGVFDTLLRHAADSSVPDKARPPYAGRHPQSGSLHNDLRQELQEKRRRLRAVL
                     EQEQYEEAARLRDAITALENTLSAIQPGEQP"
     gene            6722..7918
                     /locus_tag="PKN92_06355"
     CDS             6722..7918
                     /locus_tag="PKN92_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48451.1"
                     /translation="MTFEDFRLRYPHQRSDETVSSEIHVLSFCSLTRNLADFSFCHRC
                     SAEEKEAVQQRILQAAKTLSALKDASYFPADTVSPVDLLLFGERFLSSYRLSGTETAS
                     GVLLSEDQRIALTLNREDHLSIRVLSPQEKGMAVWEIADQVDTYLQEKLGFAFHDKWG
                     YLSPSLHRCGTGLHMVTLLNMPANALVAGRNAGDSAEAQIATLSSGLKLQYLSPGMLP
                     RCPSGYITPAVAELMAPRIEDYPCQSLYPGSSEKTAAPQATLGFYRVLTAKHVPGIHE
                     RVIAAQMDEAADALILREREARKILIERPEFLRDYVRRCLALIAQTHCLGLKEGLVCL
                     SILRLAAETGHLKNCDKQQTDLLLYAMQRAHLALSPGVDPEDPFSLAEERAKRFRTVF
                     EKAALV"
     gene            complement(7959..10886)
                     /locus_tag="PKN92_06360"
     CDS             complement(7959..10886)
                     /locus_tag="PKN92_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48452.1"
                     /translation="MVHADESITTDRRGVVVMFLRLLGITLACVLSFAAQAAEGKAAP
                     SRNREANLAAWDQAIAALIALRDGAPTQPIARMSEENTGVLTVASADLADNDGTEVPT
                     SADSFAFVFDDDEAETGIPETADEADGNTEPASPEVGETGEAEKEQEVEAVKPLLRQQ
                     PLRVGELRAPRPGRPSANPLEAMNKRTLREAITRPVEFGEASLGFEPPDWTEPMRDIE
                     ADTMTADLGKRETVLKDNVRLRLGRMRFQSDSFRFSEDEGHYEAQGNVLVEQHLSRLS
                     ADRLMYTAPADEEVEKVLVLQPGPDEQGFAKKRLSMGRLIAENLHVEEPTRALHAAWV
                     DYDFANQTGELREAQGTASVFRYTAEKVSILGPDSAIFENISLTTCPEDPPHYQLHLK
                     RLEIQDREQVRAEKLRLQFGKFKMPFYIPLVRTGASQMMALDYDSGTRAATGYYINTG
                     IQFEVTPEVSLGPRFMPTAKQGFGLGADLYYDFMSRPTSWLYRTRGELHVLYTTKDRG
                     HYLWRHRYEDNEDLVLRMESEHWSDKDYYKDFFYEESRNRTAPRTFAHLVYRQPSYFA
                     TATARVNTHPWLDESEQLPEASFHLVEQPLATPIMASFDTVSGYYRQKYRGLEGFRSV
                     NTGRLSLDWNPLPYLGVTPFIESQATWYQHLAHRDTDYFRFNHTAGLTLLTRFHKIYP
                     GILGFSAIKHVVEPSVTYSYSPRAGLSVLEAPQIDAYDSAFGRSRIESKLSNLFYGKD
                     EETGEVWRVGRVTLYHGADFWNESQKSQDYEVEIDIRPRPWWGSQFVLERHDVNGGHH
                     FIRPRRFSSLLGRTAGLYGAYSQLNAYNPYDPYNLFNRQGMYVPQLSLPERSRYDRSL
                     EQAALNIDYTRMLTQVYYDNTMLGGRLSSRVGFAYTDTAGTVYNREILYGLGYKLGDH
                     WGFSFEHIYSLRERDMIRQTYELRRRFDCWESAIRFRDRGSGFDVNLEISLVAFPGSA
                     IRF"
     gene            complement(10862..11251)
                     /locus_tag="PKN92_06365"
     CDS             complement(10862..11251)
                     /locus_tag="PKN92_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48453.1"
                     /translation="MTALIFLLTATCFGQSWDSPVITEAELAPPGAISAPAPSAPSRS
                     SDLESRTESDRTPVTELQWEYDEEGQPVARPVTGNSAQNAPKEKVSQPAAAPAPASAP
                     AEAPREKTTAKRGPRQAATFWFMLMNR"
     gene            complement(11248..12189)
                     /locus_tag="PKN92_06370"
     CDS             complement(11248..12189)
                     /locus_tag="PKN92_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNZ48454.1"
                     /translation="MSVDLISVGVVCADVMVRPVEELPPRGTLGLVPVLEMHLGGLAA
                     VTASVYAQLGGKAAFLGRVGADGFGDYLLASLQQKGVETGLVKRDPSSCSSATVVMID
                     GSGERTFLHQVGANAATCEEDIDFSRIAGLKAKALHWGGPAIMPRLDGEAMGRVFARA
                     QSQGLRTSMDTCYDGNGRWLPLIEASLPHLDLVFSSYEEACCYTGRTDTEAIADFYLG
                     YGVKIAVIKLGADGFFIKSADSTVRLPAHKVQVLDTTGAGDAFCGGFLYGWLQSWDLE
                     RCGRLANAVGGLTVQQMGGADSIRNLDQVLAFMETLS"
     gene            complement(12416..13297)
                     /locus_tag="PKN92_06375"
     CDS             complement(12416..13297)
                     /locus_tag="PKN92_06375"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HNZ48455.1"
                     /translation="MELQEIFQEFEVSRRYFPHRAVRAAVSRQEEVTPLLLRDLEAVC
                     AQAESLSKLSGSKTPYRHFFALYLLSQFREERAFSGIVDFFSLPSMYLAGLTSPLPPE
                     FPGRALASTSGGDAEKLRSLAADHACPVFLRGHALDAMKILVAEGDLPREEAIDHFRA
                     FFRGGFVRERSSVWDYLISASVRLHPEELYEDIQAAFDENLVERFFLYPKDVDTFRAI
                     SREKVMLHLRRSTPGYIRDCAKEMEGWGSFKAPKDVCSLMSPAAQLFAGKHRRPGAND
                     PCSCGSGRKYKKCCGGS"
     gene            complement(13438..>15165)
                     /locus_tag="PKN92_06380"
     CDS             complement(13438..>15165)
                     /locus_tag="PKN92_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48456.1"
                     /translation="ESPVEGELPEFFTDCVNFIGLPEGFETQANSTVTADGVVFMTSE
                     FYYSTGGSTSQGSASRGEDENGSIVLQLANINLGYDFSNTPNAPQCMHFAYGYFGGDV
                     NLEVNGDLRKVDDIAALDGLIVGNAGVRVYAKGEHTGILELKGSITTFKIGGQEFYLS
                     EICPYCEIHPLWECVTFQDLPLGMEIANGTSFISDNVLFDTGSFYYYPEGATREGTAS
                     FIEDTDGTRVLWLSNINVGHVFSDPTHLLACVSIRYRYYGGNVNLTVNNDFYNVEDIA
                     DLNGIITPGQVQVRVVPLGDFEGMLYLEGPIASFMIGGQELALLEICPYCAELPDEGE
                     GEGEGEPDEPFADCVDFGRLPDGFTAAVNLPVTIQGVTFQTSEFYYPDGSSENEGSAV
                     LSDSENGDPVLWLNNINLGYDFTAAPGLPAAISCVSISYGYFGGDINLEVNGILVAVP
                     YLAELDGTYIGAVQIRVFPKGNGIGVLQLRGAVDSFKIGGQEFYVREICPFCQEDETA
                     AHAADQNGDFIIDEDEADAYLEFWQSGHGRLSLAIRALYLRSVSDGYYDFDWTLDPPE
                     CWIPRLDVP"
     assembly_gap    15166..15265
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15266..17911)
                     /locus_tag="PKN92_06385"
     CDS             complement(<15266..17911)
                     /locus_tag="PKN92_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48457.1"
                     /translation="MRNNPTICLVALLLLTVPGLCAYGGECTDFEDLQAGTMWSTGDT
                     FTSQGFQFSLNKFFYYPSGETDSGIANPGSNEFALPDTPSSQTLWLSNVNCGLDFSNA
                     PALPQCLSFEYGVSGGNVNLEINGDLLNEETVGALDGQTAGNVLVRVYPHPDNDDYTG
                     RIDLYGNVQTLKVGGQEFYIDNLCLFCTGPEPSEGEIEGETGADFPIDLCLNFYGLSA
                     DFDAGVGEPVFILGVPFTTSEFFWLPDGSTSSGTVSVTENVIDGVPVLALDNINLGYT
                     FGSGVNPPPCISIFYVESGGNINLEINGDLRNVEDFADLNGQSVGGVSVFDFPYDGEL
                     RVLHLQGIASSFKLGGQELFLEKICPYCQEIPVSECTDFEDLQAGRTWDTGDTFTSQG
                     FQFSLTKFFYYPSGETDSGIANAGGNDSAIPGTPFSQTLWLSNVNCGLDFSNAPALPQ
                     CLSFEYGVSGGNVNLEINGDLLNEETVGALDGQTAGNVLVRVYPHPDNDDYTGRIDLY
                     GNVQTLKVGGQEFYIDNLCLSCEGDSVEGEDDPFAFFKECVDFSTMEEGLRAGNHEVL
                     NVQGVRFWMTPFFYTPEDSTADGYAQVVVGSDAYPQLVLNDINLGYDFANEPPVPSCL
                     TFSYEYNGGIVNLEVNGSLRVENDIADLNGQFVGGVLIRISQDADGRDALQLLGTVYN
                     FKIGGQNFILHEICPYCEEVDPIEGEEVPGFSVDVCLDFNDLPWIFEAQVDETVFVNG
                     VPFRTSEFFLYPQGSSRDGFVKVSMNDTGDGQILWLNNINLGYTFGPGVNPPPCLSIY
                     YDYRGGNVNLEINDDLLNVESIDQLDGKTAGNVLVKVYQKTEVTGRIELSGWIGSFKI
                     GGQELYLEKICPFCEQSPVEGEAEGE"
     gene            18057..20912
                     /locus_tag="PKN92_06390"
     CDS             18057..20912
                     /locus_tag="PKN92_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNZ48458.1"
                     /translation="MNTYGNRLLKCTAAFAFAFAVLLLVGCGDKTPPGAVTMFAAQSG
                     DGEITLSWVNPPDKDLAGVRVVRSQSAPPAKPSEGLEIFSDSGTGLVDGNVTNGNPYF
                     YAAWAYDRAGNHSSPVYASATPVSFQAREEILDKLDSMAEQIAAIPTLTEEEKKEMQD
                     ILDETEDLFLGGDPCGAAAVMKDEFLEKCQWVRQTRERPEGEKLYAAGRMVRVNIART
                     MEAKGECDELQRVDLEAEIQVESEDPEGLSGWSVFGEPLLTALELHENTAPESTFTQV
                     FIPGAEAVHGQVGAPDIPVYRQLVAVPMGDDVKVKILQQRPVIAEEIFLNLYPVQPAP
                     MDQGPDLSLFKDPPFTINHSIYESNEPWPPEPVTMRYIGNGRDLEFYLLEMASGQYYP
                     AENRLELFDYTHLDVEFQGGPGHFATSHMISPFESNSRALIESAINKEVIKENIIEGI
                     RQDIIGEELLILTHPNFYDAAIKLRDWKRSKGIWANVYECGTNSDIHWRATGEQIDAF
                     IEERYHTTEIRVSYVLLLGDAEFIPTFYINNIGTDWPYAILGKPGEDLIADFAVGRIP
                     VDTLDQAMTVVDKTINYEKTPIDDKDFYQNAVLASQFQCCREKAPDQGTDSRTFIQCS
                     EFAQQMLSAAGKTVSRIYARTGSQTPNRYYDGTLLPSALRPSAKFPWDGNTNQITEAW
                     NKGAFLIIHRDHGEPHGWETPRFRSSHIDNLENEDRLPVVFSMNCSTGFFDNETAGGA
                     GGTVANDVYFCERALRKPDGGAVGIFGATRISPSWENTALTMGMMDAIWPGRLNFGFS
                     TLSQRRLGDILNHGKRYILSMRGVSVMGEDLFEDSVIEELYLWHCFGDPTLEIWTKNP
                     YSQTNPFSPVFHHQGLAVQDGIDISGGILVEYGVDNAVITVFERGADQEIPLGRGVVQ
                     NGIAQITYLQNHVMDHELTIIASFDNAPAKVLQGNSF"
BASE COUNT         5088 a         6052 c         5213 g         4483 t
ORIGIN      
        1 gaaaccggga ctgctgattg accaccgatc agacgtttgt gttcggcaac cagcgcatcc
       61 accacagacg gatctgccag cgtcgttgta tcgccgatct ggtcggtggc gttttccgca
      121 atcttgcgca ggatacggcg catgatttta ccggaacgcg ttttcggcag agccggagca
      181 aactggatca catcaggagc cgcaatgggt ccgatttccg tacggacatg cttcaccagc
      241 tctttgcgga gttcttcgct ctcctccaca ttctcgcgca gcgtcacata agcgtacagc
      301 gcctgacctt taatatcgtg cggcatgggc gccacagcgg cctccgccac tttcggatgg
      361 ctcacaagcg cactctccac ttccgccgtg ccgattcggt gaccggacac attcaccacg
      421 tcgtcaatgc ggcccatcag ccagtaatcg ccgtccgcat ctttgcggca cccgtcaccg
      481 gagaagtaaa gatcgctgaa catggtgaaa taggtatcga taaaacgatc gtgatcgccc
      541 cacatggtac gcatcatgcc cggccagggt ttgcggatgc acagcttacc gccttcctcg
      601 agtccgactt ccgtgcggtc atcacgaagg atcaccggat ccacaccgaa gaagggacgg
      661 ttggcactgc cgggcttcag cgtgaagcag gggaaaccct ggatcatgaa accgcccgtc
      721 tccgtctgcc accaggtatc cataatgggg cagcggcttt tgccgacaac cgtgtagtac
      781 cacatccacg cttcaggatt gatgggctca cccacggagc cgagaatacg cagggaactc
      841 agatcatatt tgttgaccca ttcatcgccc ttctggataa gcgcacggat cgccgtcggc
      901 gccgtgtaaa tctgcgtaca ctggaactta tcgacgatat gccagaagcg ccccggatcc
      961 ggataggtcg gaacgccttc gaacataacc gacgtcgcac cgttggcgag ggggccgtat
     1021 acaatgtaac tgtgacccgt tacccagccg atatccgccg tgcaccagta aatgtcgtct
     1081 tcacgcaaat caaagacata tttatgtgtc atggcaacct gaagcagaaa gccacccgtc
     1141 gtatgcacca cgcctttggg tttaccggtg gagccggagg tgtagaggat gaacagcgga
     1201 tcttccgcat tcagagcgac aggctcgcaa acctcactgg cttcctccat gaggtcgtcg
     1261 taccagaggt cacggccttc ctgcatgctg cagggttccg cattccgttt tacaaccacg
     1321 caatgctcaa tggtcttgca gtccggcttc gccatggctt catcggcaat cgctttcagt
     1381 ccgatatgct ttccgcttcg aagcgacacg ttagaagtga tcagcagctt gcagctcgca
     1441 tcgcctacac gggtcgcaat ggcatcagca ctgaaaccgc cgaagacaat ggagtgaatc
     1501 gcaccgatgc gggtgcaggc aagcatcaca ataggcagct ccagaatcat gggcagataa
     1561 atgcagacgc ggtcgccttt cttgacccca agccctttca gcacattcgc aaatttacag
     1621 acttcatagt gaagttcttt gtaggtaatt tcccgcacgt cgtcttcagg ctcgccctgc
     1681 cagatcagcg caattttgtc ttcgcgctcc gtgcccagat gccggtcaag gcagttgtac
     1741 gatacattca gctcgccgtc tttaaaccag gtgtgctcga taagccggcg ttccgtatcc
     1801 cagatgtact ctagagattt cgtgggctct ttaaaccagc ttaattcttt cttcgcatgc
     1861 tccagccaga atgtatccgg atcattgacg gaagcctgcc acatcgcctg atactccgct
     1921 tcgctcttca ccagagcatt ggcacggatt tcctcgggcg cgggaaatgt acggttttcc
     1981 gtcagcaagg aactcaaact ttttttctct tcagttccca tagcaatacc tttcttcagt
     2041 tgttgctttg tattcacaac cgcagctcaa aaggagcctt ggtcacacta cgcaatctga
     2101 tcgagaaatc tggcacacac aagaaaaact ggtcgaacaa gtgtatacct accgttcgtt
     2161 aggcagaagt atactaaaca acaaattgtt ctttcaaatt aattccggga cgaatgtttt
     2221 ttgtcttaaa aaatacatcc gacaataata tgaaaatact gcagacgcaa agaaaaggga
     2281 gggattgact gaaaaaacag agacacctca cccctaagca gggcgatccg ttaaaaaagg
     2341 tcgatccgga gcggaatcat gcagcccgtg tcagggagta atgcgaagtg cgttttcatc
     2401 tgcctgcacc gtcagcccgc ccgtctgtac ggtcactgta cctgaagcgt ccgtactgca
     2461 ccgtgcggca tcgggcacgg cgtctccttc gagcagatgg aaaactgtaa caaagactgc
     2521 ctcttttcct gacagacggc gcatcagcat gggaagattc tcctcgtact gatacccggg
     2581 actgcgcccg acaaactgct gcccggcatc cgcgatctgc agcacaatgc ggctggacgc
     2641 accggttgcc acagtatacc agtctgcatg cgacactgct tccttcacct cacgaaattc
     2701 cctgtagccc ggcgcctcaa aagaaaccgc ctcctctttc atttccggca gatccgccca
     2761 ttcgccgcga aaatggagcg gcagatcaaa gagcgcatct tcttttgacc tgcagcggaa
     2821 aatatcgatc accgctttat cggcaagcac tacagtgcgg ttcagcgcat acttttcacc
     2881 atccaatgtt gccatagccc gggcagcacg ccagccctcg ccgacttcaa aaaaagtaca
     2941 ggtcgattca atgcgcttgt ggctcgtttc attcaccaca accgtgttat gcgacaccgt
     3001 ctgacggcac cattccttat ggagcgggtt tccgtaaggc agacgtcccg cctccgccag
     3061 gcgctcttca ccctgagcaa ataaaagaag attaaccgtt gccgggtgca tatgccctac
     3121 ccgtgcacgg gtgtaatcaa gaaaaacggc tgtcttgccg tccggattgc gcagcacggc
     3181 aagcgcctct tcatcatcat tatacgagtc aagtgtcaac gccccttttt cttccggcag
     3241 gctgtctgcg ccgtaaagca gcgcatctct gctgtcccga acagtcagca gcggaagata
     3301 ccgttcgtcc ttgaaatgac tgtagcccat ttcataaaaa ccgcgattgc ctaaaatgga
     3361 cgtacgcggg ctgtcatgga tggcgggaaa agtcagatcc gggtacagct gccgcagcgg
     3421 tccatcgagc atccttttca caggcggaag gttccagaga ttcatgcctg cccgacccgc
     3481 cgcttccagc agataataga gcgcatgaaa ggaataccag tgatagccgg gtgctccttc
     3541 ataccacatg ccgccggaaa gaacggagcg ctccatctga aacatgagcc cggattttcc
     3601 gttgattgcc cagtctacaa gctcccgttc tccggtaagc agaccggcgc agcccactgc
     3661 cgaattatgc caggtctgcc agttcgaaat ccgcatgtca taacgctgaa tggtctcgca
     3721 catgggcagc agcagatgtt cttggataat ccggtgatct tcttcagaaa aacagggatc
     3781 cccgtaaacc agatcataag cgaaacagaa cttgcagaga ttcacggatt catcaagtgt
     3841 ctgcgcatac agccgtgctg ccgaaagcgt cgtattaccg tcacggtcgt gcagcggaag
     3901 gtcttcataa aaagacgcat attcaagaag cgaatcacgg atatgctttg ccctgacccc
     3961 ggtcggctca atcacatagg caatggcgat ggtctgcaga ttattgttgt accggttgtg
     4021 catataggtg atataggcat catcgtaagg ataacctgaa tagacttcgc cgcaaacgga
     4081 acagcggtga gcggtcggcg tgtccgtgat aaacgaaccg ccgtctttcg gacagctgta
     4141 ccagtgcgtc cactgaccgc cctgatgcgg aatggcagga acattttcca ggcagctgtc
     4201 tgcttctttc agcagaccag cacactgctt ctcccattgg ggatctttcg cacgggcacg
     4261 aagacgcatc atctcgtctt cggtcatgaa aagacggggc ggtgtccctg cgggcatgac
     4321 aatatccaat gctccggaag aaaccgacac cagaaaacag atcagaagca gcacgatacc
     4381 actgcgaaag gatgaagata cagccatgac acactcccta aaagcaaaaa caacagtagt
     4441 atctcattga ctggctgccg gaggaaaagt ttcacgcaac acccgtcatc tccgcaggag
     4501 gtaccggccg taccagtgcc gtctcatcgc actgagcgaa aaagggacag cgctgacagt
     4561 ctttcaaggc tttgtaaggc agttctgccc gatccaccac ttcgaagcca agccgtttaa
     4621 aaaattccgg ctgccgggta aaagcatata cccgggcaat ctgaagctgg cgcgcctctt
     4681 ccactgtcgc ttccaccagc atactgccga tccccctgcc ccgaagtctg tcccgcacca
     4741 cgagagaacg gacttccgcc agatccacca gatctatatg aagtgccgcc atcccgccca
     4801 cgccctcttc atccacatac acaaaaaaat cgcgcgcatt ttcaagcatt tctgacagct
     4861 cacggggcag cacctgccgg gcggctgccg cctcatccag aagcgctttc atatcggcga
     4921 tttcggtaag tttgggttta cgtacaatgc ccatgacatc tgtatccatt gttgaaaata
     4981 agtgtggcta taaagagggc agcatttgca gtgcggcaag ggcggcatca tgaagcacgc
     5041 cgttggatgc gacaacgccc gtattcttca gtaaccgcct gccctgtgaa aaatccagcc
     5101 tgtccccctt cagatcggtc acacggccac ctgcttcctc caccaccagc gaacccgcag
     5161 cctggtccca aatatattct ttgtaaccgg gacgctccgg cgacagaagc cggaaaataa
     5221 actcagcacc gcccgatgcc agcacagcaa atttcgcctg gctgtccata aggacattgg
     5281 cttccggtct cagattgaga gcgtccgcaa gctgctccaa agcggatacg tctgtgtgac
     5341 ctgcttcaaa agagcggagc atgcgcgcct tcgaaggatc gctgcagcca ttcacatgga
     5401 gcgctgcagc cgaggaggga ccagctgccg aagatatagg aaagagccat gctccggcgc
     5461 ccctttttgc cgcagcgacc agccccgtgc ctgcaaacgc aggaagttca ccagcaccca
     5521 atgcagggca ggcaagaccg ccccggatca catggccatc ctctatctga gccagtgcca
     5581 cagcgtactg tccgccccgg cggtagcctt tggttccgtc gatgggatca agcgtccaga
     5641 aaagcgcgcc gccttctgac gccgcgccct gatcaatcca gtcacagacc gccccgcccg
     5701 tcgccccggg gataaaccgg gatacaaaag aggtaattac ctccagcatg tcacagccgt
     5761 cgggggcacg cagagaggag gcgtcttctt ccgccacaag caccgcatcg ggaaaaacgt
     5821 cgccaagccg ccgggatacc agtgcctgta tcgcaaaatc cgccactgtc accggcgaca
     5881 gatcgctttt ttccactgta aacagtgcgg actcttcccg taccgcgacg gcaagcatag
     5941 ccgcttcacg gagcacagat agaatcagcc ctgtttcctg atcaaaaaat tcagccatca
     6001 cagctttcac ccgttcatac gcccctctcc gcattccgca gtagtgtacc ataccgcatg
     6061 aatccgcccc aaaagggatg tttttaaaaa aagccatcct gctggagtct cctttccatc
     6121 cgtggtatac ttgcctcagg aatttacata tgccttgtga tcattgcaaa aaagataaag
     6181 aactcaaaag ttatgccgaa gtgaaggacg ggcagatcta ccgcttgtcc ctttgcgagg
     6241 aatgccttgc cctttatctc tcaaaggcac cggagttttc ccggaatgcc ttttctcgta
     6301 ccgcagcccc gaagaaggca aaagtacgca gggaagattc tccgacaggt ctaaaagcga
     6361 ctcccgaaaa agaggcgact agccgcccgc ctgacatccc ggagacatgc cccgactgcg
     6421 aaacttcgtg gctgcaggca aagaaagggc gtcccggatg tcctggttgt ttcggcgtat
     6481 tcgacacttt gctgcggcat gcggcagaca gcagcgtacc cgataaagca cgaccgcctt
     6541 atgcaggcag gcatccgcag agcggcagtc tgcacaatga tcttcgacag gaactgcagg
     6601 aaaaaagacg acggctccgt gcagtgctgg agcaggagca gtacgaagaa gcggcacggc
     6661 tccgtgacgc aattacggca ctggaaaaca ccctgtccgc tatccagccc ggtgaacagc
     6721 catgaccttt gaggatttcc ggcttcgcta tccacaccag cgctctgatg aaacagtctc
     6781 ttcagagatt catgtccttt ctttctgctc cctcacgcgg aatctggcgg atttctcttt
     6841 ctgccatcgc tgttctgctg aagaaaaaga agccgtgcag cagagaattc tgcaggctgc
     6901 caaaacattg tcagccctga aagatgcctc ctatttccct gcagacactg tgtctcctgt
     6961 cgatttgctg cttttcggcg agcgtttttt aagcagctac cgtctgagcg gcaccgagac
     7021 agcaagcggc gtcctcctct ctgaagatca gcggattgcc cttaccctca atcgcgaaga
     7081 ccacctttcg atccgtgtgc tgtccccgca ggaaaaaggc atggctgtct gggagatcgc
     7141 cgatcaggtg gatacatacc tgcaggaaaa actgggcttt gcctttcacg acaagtgggg
     7201 atatctgagc ccgtcgctgc accggtgcgg cacaggtctt cacatggtca ccctcctcaa
     7261 catgcccgcc aatgcgcttg ttgcaggaag aaatgcgggc gacagtgcgg aggcccaaat
     7321 agccacgctg agcagcggtc tgaaattaca gtatctgtcg ccaggcatgt tgccccgttg
     7381 cccttcaggc tatatcactc ctgccgttgc ggagctcatg gcgccgagaa ttgaggatta
     7441 tccctgccag tctctttatc cgggcagcag cgaaaaaaca gccgcgcctc aggcgaccct
     7501 gggcttctac cgggttctga ctgcaaagca tgttccaggc atccacgaaa gagtaattgc
     7561 ggcgcagatg gacgaagcgg cggatgccct gattctgaga gaacgggaag cccggaaaat
     7621 actgatcgaa cgccccgaat tcctccgcga ttatgtgcgg cgctgcctgg ctctgattgc
     7681 gcaaacgcac tgtctgggat tgaaagaagg gctggtatgc ctttccatcc tacggctggc
     7741 agcagaaaca gggcatctaa aaaactgcga taaacagcag accgaccttc ttctgtatgc
     7801 catgcagcgg gcgcatcttg ccctgtcgcc cggagtagat ccggaagatc ccttttctct
     7861 ggcggaagaa cgggcaaagc ggttccgtac tgtttttgaa aaggcagcgt tggtataagc
     7921 ggcaacggcg cctctacaga accctccacc cgtattactc agaagcggat agcacttccc
     7981 gggaacgcca ccagactgat ctccagattc acgtcaaagc ctgaacccct gtcccggaag
     8041 cggatggcag attcccagca gtcgaagcgg cggcgcagct cataggtctg gcggatcata
     8101 tcgcgctcgc gcaggctgta gatatgctca aaactgaagc cccaatgatc ccccagttta
     8161 taccccagcc cgtacagaat ctcccggtta tagacagtgc ccgccgtatc cgtataagca
     8221 aagccaaccc ggctgctgag ccgtccgcca agcatggtat tgtcgtaata cacctgtgtc
     8281 agcatacggg tataatcgat attaagtgct gcctgctcaa ggctgcggtc atagcggctt
     8341 ctttcaggca gactgagctg agggacatac attccctgcc ggttaaacag attataagga
     8401 tcataaggat tgtacgcatt cagctgactg taggcaccat agagtccggc agtgcgcccg
     8461 agcagcgatg agaatcgccg cggtctgata aaatggtgac ctccgttgac atcgtgccgt
     8521 tccagaacaa actggctgcc ccaccagggc ctcggccgta tatctatttc gacttcataa
     8581 tcctgcgatt tctgagattc attccagaag tccgctccat gatacagcgt caccctgccg
     8641 acccgccaca cttctcctgt ctcctcatct tttccataga aaagattgga gagtttggac
     8701 tcgatgcggc tgcgcccgaa tgcactgtcg taagcatcaa tctgaggtgc ttccagcaca
     8761 ctgagtcccg cacgggggct gtaggaatag gtgactgaag gctccaccac atgcttgatc
     8821 gccgagaaac ctaaaatgcc tgggtaaatt ttgtggaacc gggtcagcag ggtcagcccg
     8881 gctgtgtgat tgaaccgaaa ataatccgta tctctgtgcg caagatgctg gtaccaggtc
     8941 gcctgacttt caataaaggg ggtgacgccc agataaggca gcggattcca gtcgagactg
     9001 aggcgaccgg tgttgaccga gcggaaccct tccagccccc ggtatttctg acggtaatag
     9061 ccgctgaccg tatcaaaaga agccataatc ggtgtcgcca gcggctgctc aacgagatga
     9121 aaactcgctt caggaagctg ttctgattca tccaaccagg gatgcgtatt gacccgggcc
     9181 gtggcggtgg caaaatacga cggctggcgg tacacgagat gcgcaaacgt gcgcggtgcc
     9241 gtcctgtttc tggactcttc gtaaaagaag tccttatagt aatccttgtc cgaccagtgc
     9301 tccgattcca tgcgcagcac gagatcctcg ttatcctcat agcggtgccg ccacagataa
     9361 tgccctctgt ctttggtggt atataagacg tgcagttcac cccgcgtcct gtaaagccat
     9421 gacgtgggcc ttgacatgaa atcataatag agatctgcac ccagcccgaa cccctgtttt
     9481 gccgtgggca tgaatctcgg ccccagcgac acttcaggcg tcacttcgaa ctgtatgccc
     9541 gtgttgatgt aatagcccgt tgccgcccgg gttccgctgt cataatccag tgccatcatc
     9601 tgactcgcac cggtacggac gaggggtata tagaagggca tcttgaactt gccaaactgc
     9661 aggcgtaatt tttctgcgcg cacctgctcc cgatcctgaa tttcaagccg cttcagatgc
     9721 agctggtaat ggggcgggtc ttccgggcag gtcgttagcg agatattctc aaatatagcg
     9781 ctgtccgggc ctagaatact cactttctcc gccgtatagc gaaacacgct tgccgtacct
     9841 tgcgcctccc gcaattcacc ggtctggttc gcaaaatcat aatcgaccca ggccgcatga
     9901 agcgcacgag tcggctcctc cacatgcagg ttctccgcaa taagccgtcc catagacagg
     9961 cgcttctttg caaaaccctg ttcatccgga cccggttgca gcaccagcac cttttccact
    10021 tcctcgtctg ccggtgccgt gtacatgagt ctgtcagcac tgagccggga cagatgctgc
    10081 tccacgagca cattgccctg cgcttcgtag tgcccttcgt cctcggaaaa acggaaggaa
    10141 tccgactgaa aacgcatcct gcccagacgc agccgcacat tgtctttaag aaccgtttcc
    10201 cgcttcccca gatccgccgt catggtgtcc gcttcaatat cacgcatcgg ctctgtccag
    10261 tcgggcggct caaaaccaag tgatgcttca ccgaattcca ccgggcgtgt aatcgcttcg
    10321 cgcagggtgc gcttgttcat ggcttccagc ggattggcag aagggcgccc cggcctcggt
    10381 gcacggagct ctcccacgcg caggggctgt tgccggagca gcggcttcac ggcttccacc
    10441 tcctgctcct tttccgcctc tcctgtttca ccaacttccg gagaagcagg ctccgtattc
    10501 ccgtcggctt cgtcggcagt ctccggtatt cctgtttctg cctcatcgtc atcgaaaaca
    10561 aaggcaaagg aatctgcaga cgtcggcacc tctgttccgt cgttgtccgc aagatccgca
    10621 cttgccaccg tcagcacccc ggtattctct tcagacatgc gggcaatcgg ctgtgttggc
    10681 gccccatcac gcaaggcgat cagtgcggca atcgcctgat cccaggcagc aagattggct
    10741 tcccggttgc gggacggcgc cgccttaccc tccgcggcct gagcggcgaa ggaaaggaca
    10801 cacgccaacg ttattccgag cagtcgaaga aacattacga ccacgcctcg tctgtctgtg
    10861 gttatcgatt catcagcatg aaccaaaaag tcgccgcctg acggggcccc cgttttgccg
    10921 tcgttttttc acggggtgcc tctgccggag cagatgcagg cgcaggtgcc gcagcgggct
    10981 gcgacacctt ttcttttggt gcattttggg cagaattccc tgttaccgga cgggcaacag
    11041 gctgtccctc ttcatcatat tcccactgca gttctgtcac cggcgtgcgg tcggattccg
    11101 tacgcgattc gagatcactt gagcgggacg gcgccgaagg ggcgggtgcg ctgattgctc
    11161 caggaggggc aagttccgcc tctgtaatca ccggactatc ccagctctgc ccgaaacagg
    11221 tggctgtcaa cagaaaaatc agcgctgtca tgacaatgtc tccataaacg ccagaacctg
    11281 atcaagatta cggatgctgt ccgctccgcc catctgctgc accgtcagac cgcccaccgc
    11341 attggcaagc ctgccgcagc gttcaagatc ccacgattgc agccagccgt acagaaaacc
    11401 gccgcaaaaa gcatcgcctg cgccggtcgt atccagcact tgtaccttat gcgctggcag
    11461 ccggactgta ctgtcagcac ttttaatgaa aaaaccatcg gcacccagtt tgatcacagc
    11521 gatcttgacg ccatagccca aataaaaatc ggcaatggct tccgtgtctg tcctgcctgt
    11581 atagcagcac gcttcctcat agctggaaaa gaccagatcc agatgaggca gcgatgcttc
    11641 aataagcggc agccatctgc cgtttccgtc gtaacaggta tccatgctgg tccgcagtcc
    11701 ctgtgactgg gcacgggcaa ataccctgcc catcgcctct ccatccaggc ggggcataat
    11761 ggccgggccg ccccagtgaa gcgcttttgc tttcagaccg gcaatgcggg aaaaatcgat
    11821 atcttcctcg caggtggcgg cattggctcc gacctgatga agaaaggtgc gttcccctga
    11881 accgtcaatc attactacgg tcgccgaact gcaggaggaa gggtctcttt ttaccaaccc
    11941 tgtttctacc cccttctgct gaagactggc aaggagatag tcgccgaagc cgtcagcacc
    12001 taccctgccg agaaaggccg ctttgccacc cagctgggca taaacactgg cggtaacggc
    12061 ggcaaggccg cccagatgca tttcgagtac aggcaccagc cccagcgttc cacgcggagg
    12121 cagttcttcg accggacgca ccatgacgtc ggcacaaaca acgcccaccg atatcagatc
    12181 tacagacatg gacacccctt tctagcgggt gatcaggcaa aggaacagat cgaatgaaac
    12241 aacagcatgg cggcgcatct gaaaatcagc cctgaacgca gctgcaccgc cggtgtttgc
    12301 ggatattccg gagaagccgc aaacacagtt cagccgaaga aagtatagca ggaagcacag
    12361 aggcaagtaa acacattttc caccctcttt tttacaaaat ctgctgaaaa tcaggtcatg
    12421 acccgccgca gcattttttg tattttctgc cgctgccgca gctgcacggg tcattcgcac
    12481 cgggacgccg gtgctttccg gcgaaaagct gcgctgcagg agacatgagc gaacaaacat
    12541 ctttcggcgc tttaaaactg ccccatcctt ccatttcctt tgcacagtcc cggatataac
    12601 ccggtgtgga acggcgcaga tgcagcatta ctttttcccg gctgatcgct ctgaaagtat
    12661 ccacatcttt cggatacaga aaaaagcgtt ccacaagatt ctcatcaaaa gccgcctgaa
    12721 tatcctcata caattcttca ggatgcagcc gaaccgatgc gctgatcagg tagtcccaca
    12781 ccgaggaacg ctcccggaca aatccgcccc gaaaaaaggc ccggaaatgg tctatcgctt
    12841 cctcccgggg caaatcacct tccgccacca ggatcttcat cgcatccagg gcgtgcccgc
    12901 gcaaaaatac cgggcaggca tgatccgccg ccaatgaacg aagcttttct gcatcgcccc
    12961 cgctcgtgga tgccagcgca cgccccggaa actccggggg cagcgggctg gtcagccccg
    13021 caagatacat cgagggaagc gaaaaaaagt caactatccc ggaaaaggca cgttcttccc
    13081 ggaattggga caacagataa agcgcaaaaa aatgacggta cggcgtcttt gagccggaaa
    13141 gcttgctgag cgactccgcc tgggcgcaga ccgcttccag atcccgcagc agcagagggg
    13201 tcacctcttc ctgacgggaa accgccgccc gaactgcccg atgcgggaag tagcgtcggc
    13261 tcacctcaaa ttcctgaaaa atttcctgaa gttccatggg ctctcttctc agtccctttt
    13321 gcttttctct tcattatggc ttaccgccga aggaattata cataaaaaga ccggctgtcc
    13381 tctccggaac ctgcccggaa aaaacagccg gtcgaaatgg atcacagaaa aaacgggtca
    13441 gggaacgtca agacggggga tccagcactc gggcggatca agcgtccagt caaagtcgta
    13501 gtagccgtcg ctgacagagc gcagatacag ggcacggatt gccagcgaca gtctgccgtg
    13561 acccgactgc cagaattcaa gataggcgtc cgcttcatcc tcatcaatga tgaagtctcc
    13621 gttctgatcc gcagcatggg cagccgtctc gtcttcctgg cagaaggggc agatctcacg
    13681 cacataaaac tcctgaccgc caatcttgaa gctgtcaaca gcaccccgaa gctgaaggac
    13741 tcctattccg ttccctttgg ggaacacacg aatctgtacg gctccgatat aggttccatc
    13801 cagttcagca agataaggaa cggcaacaag aataccattc acttccagat tgatgtcgcc
    13861 gccgaagtag ccgtaagaaa tactcacaca gctgatcgcc gcaggcagac cgggcgctgc
    13921 tgtaaagtca taaccaagat tgatattatt cagccagagc acaggatcac cgttttcact
    13981 gtcactcagg acagcagacc cttcgttctc ggaagagccg tccgggtaat aaaattcact
    14041 ggtctggaaa gtgacaccct gaatcgtcac agggaggttc actgcggccg taaagccgtc
    14101 cgggagccgt ccgaaatcaa cacagtccgc aaagggctca tccggctcgc cttcaccttc
    14161 tccttctccc tcatccggca gttccgcaca gtaggggcag atttccagca gggcaagttc
    14221 ctgaccgcca atcatgaagg aggcaatcgg gccttccaga tacagcatac cttcgaaatc
    14281 gccgagggga acaacacgca cctgcacctg accgggcgtt atgataccgt tcaggtcggc
    14341 aatatcttcc acgttataga agtcgttgtt cactgtcaaa ttcacattgc cgccgtagta
    14401 tctgtaacgg atgctgacgc aggcaagcag atgggtggga tctgaaaaca catgacccac
    14461 gttgatatta ctcagccaga gaacacgcgt gccgtcggta tcttcaataa acgacgcagt
    14521 gccttcccgt gtagcgcctt ctgggtagta gtagaaagaa ccggtgtcaa aaaggacatt
    14581 atcactgatg aagctggttc cgtttgcaat ttccatccca agcggcagat cctggaaagt
    14641 cacacattcc cacaagggat gaatttcaca gtacggacag atttcagaca gataaaactc
    14701 ctgtccgccg atcttaaaag tggtgatact tcctttcagt tccagaatgc ccgtatgctc
    14761 tcctttggca tacacacgga cgccggcgtt gcctacaatc aggccgtcca gcgccgctat
    14821 atcatccact ttccgaagat ctccgttgac ctccagattc acatctccgc cgaaataccc
    14881 gtaggcaaag tgcatgcact gaggtgcatt aggcgtattg gaaaaatcat atcccagatt
    14941 gatattagcc agctgcagga caatgctgcc gttctcgtct tcaccccgtg atgcggaacc
    15001 ctgactggtg ctgccaccgg tgctgtaata aaactcgctg gtcatgaaaa ctacgccgtc
    15061 agctgtcacg gtgctgttcg cctgcgtttc aaacccttcg ggcagaccga tgaaattcac
    15121 acagtccgta aagaactccg gcaattcgcc ttctaccgga gactcnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnttcgc cttcggcttc cccttctaca ggcgactgct
    15301 cacagaaagg acagattttc tccagataca gctcctgacc gcctattttg aaactgccga
    15361 tccatccgga aagttcaatc ctgcccgtaa cttccgtctt ctggtagacc tttacaagaa
    15421 cattaccggc agtttttccg tcaagctgat ctatactctc cacattcagc agatcatcgt
    15481 taatctcaag attcacattg ccgccgcggt agtcataata aatactgagg cacggaggcg
    15541 ggttcacacc cggaccgaag gtatatccta aattgatgtt gttcagccac agaatctgcc
    15601 cgtccccagt atcattcatg cttactttga caaaaccgtc cctggatgag ccctgcggat
    15661 acaggaagaa ttcgctggtt cggaaaggga ctccattaac aaaaacggtc tcatccacct
    15721 gtgcttcaaa aatccagggg agatcgttga aatcaaggca gacgtcaaca gaaaagccgg
    15781 gcacctcttc gccttcgatc gggtcaacct cctcacagta ggggcaaatc tcatggagaa
    15841 tgaagttctg gccgccaatc ttgaaattgt agaccgtccc cagaagctga agcgcgtccc
    15901 gtccatccgc atcctgtgaa atgcggatga gcacgcctcc cacgaactgt ccgttcaggt
    15961 cagcaatatc gttttccacc cggagcgagc cgttgacctc cagattaaca atacctccgt
    16021 tatactcata ggagaaagtc aggcaggaag gaacgggcgg ctcattggcg aaatcatacc
    16081 ccagattaat atcgttcaga accaactggg gataagcgtc gctgcccaca actacctggg
    16141 cgtatccgtc agccgtactg tcttccggcg tgtaaaagaa cggggtcatc caaaaccgca
    16201 cgccttgcac attcaaaacc tcatgattac ctgcccggag accttcctcc atcgtgctga
    16261 aatcaacaca ttctttgaag aaagcaaaag gatcgtcttc accttcgaca gaatcaccct
    16321 cgcaggacag gcaaagattg tcaatgtaga actcctgtcc gcccactttg agggtctgca
    16381 cgttgccgta caaatcgatt ctgccggtat agtcgtcatt atcaggatgc ggatagaccc
    16441 tcaccagaac attgccggca gtctgaccgt caagagcccc caccgtttct tcattcagaa
    16501 gatcgccgtt gatctccaga ttcacattac cgcccgatac tccatactca aagctcaggc
    16561 attgcggaag tgcaggtgca ttggaaaagt caagcccgca gttgacattg gacagccaca
    16621 gtgtctggct gaagggggta ccgggaatgg cagagtcatt accgcccgca ttggcaattc
    16681 ccgaatctgt ttcgcccgac ggatagtaga aaaacttggt caggctgaac tgaaaaccct
    16741 ggctcgtgaa ggtatcacct gtgtcccacg tcctgcctgc ctgaagatct tcaaagtccg
    16801 tgcattcact gacaggaatt tcctggcaat aggggcaaat cttttccaga aaaagctcct
    16861 gtccgcccaa tttgaaagaa gaagcgattc cctgcagatg caaaacgcga agttcgccat
    16921 catacggaaa atcgaaaacg gacacaccac ccacagattg accgttcagg tcggcgaaat
    16981 cctccacatt gcggagatca ccgtttatct ccagatttat gttgcctccg gattcaacgt
    17041 agaaaatact gatgcagggc ggcggattca cgcccgaacc gaaggtatac cccaaattaa
    17101 tattatccaa tgctaaaaca ggtacgccat cgatgacatt ctctgtaacg gagactgtgc
    17161 cgcttgacgt ggatccatcg gggagccaga aaaattcact tgtcgtgaaa ggaacgccga
    17221 ggataaacac aggttcaccg acaccggcgt caaaatcggc ggacagaccg tagaaattca
    17281 gacaaagatc tatcggaaag tctgctcccg tttcgccttc gatttcgcct tcactcggtt
    17341 caggtcctgt acagaaaaga caaagattgt caatatagaa ctcctgtccg cccactttga
    17401 gggtctgcac gttgccgtac aaatcgattc tgccggtata gtcgtcatta tcaggatgcg
    17461 gatagaccct caccagaaca ttgccggcag tctgaccgtc aagagccccc accgtttctt
    17521 cattcagaag atcgccgttg atctccagat tcacattacc gcccgatact ccatactcaa
    17581 agctcaggca ttgcggaagt gcaggtgcat tggaaaagtc aagcccgcag ttgacattgg
    17641 acagccacag cgtctgactg gacggggtat cgggaagggc aaattcattg ctgcccggat
    17701 tggcaatccc cgaatctgtt tcgcccgacg gatagtagaa aaatttgttc aggctgaatt
    17761 gaaagccctg actcgtgaag gtatcacctg tgctccacat ggtgcctgcc tgaaggtctt
    17821 cgaagtcggt gcattctcct ccgtaggcgc aaagccccgg tacagtgagc aaaagaaggg
    17881 caactaagca aattgtaggg ttgtttcgca ttattctgtc cttttttggt tattggtgta
    17941 taataagtac aatgttatta taacacatac cagcggtagg gcaagtacaa aagagcgttc
    18001 gaaaacactt ttattttttt ggaagtttcc actttgaaca gcaggaggat tgaagaatga
    18061 atacctatgg taatcgttta ctgaaatgca ccgccgcttt cgcttttgcg ttcgctgtac
    18121 tgctcctggt cggatgcggt gacaaaacac cgccgggtgc tgtaaccatg ttcgcggcac
    18181 aaagcggcga cggtgaaata acactctcct gggtaaaccc gcccgacaaa gacctggcag
    18241 gggtcagagt cgtccgaagt cagagcgctc cgcctgcaaa gccttcagag ggtcttgaga
    18301 tcttcagcga ttcaggaacc ggtctggtgg acggcaatgt caccaacggc aacccttatt
    18361 tctatgccgc ctgggcttat gaccgcgcag gcaatcactc cagccccgtc tatgccagcg
    18421 ccacacccgt atcttttcag gcgcgggaag aaatactgga caaactcgac agcatggcag
    18481 aacagattgc ggccattccc acgctgacag aagaagaaaa gaaggagatg caggatattc
    18541 tggatgaaac agaagatctc tttctcggtg gtgatccctg tggcgctgca gcggtaatga
    18601 aagatgaatt tctggagaag tgccaatggg ttcgccagac cagagaacgc ccggaaggcg
    18661 aaaaactcta tgccgccggc agaatggttc gtgtgaatat cgcccgcacc atggaagcca
    18721 aaggtgaatg cgacgaactg cagcgggtgg accttgaagc ggaaatacag gttgaatctg
    18781 aagatcctga agggctttcc gggtggagcg tttttggcga accgctgctg accgccctgg
    18841 aactgcacga aaacacagcg cctgaaagca cattcacgca agtgttcata cccggcgccg
    18901 aagccgttca cgggcaggtg ggtgcccccg atattccggt ctaccggcag ctcgtggctg
    18961 tgcccatggg cgatgacgtc aaggtgaaga tcctccagca gagaccggtg attgccgaag
    19021 aaatttttct gaatctctac ccggtacagc ccgcgcctat ggatcaggga ccggacctct
    19081 cgctctttaa agatccgccc ttcacgatta accacagcat ctatgaaagc aacgagccct
    19141 ggccgcccga gcctgttacc atgcgctaca taggcaacgg ccgggatctg gaattctatc
    19201 tgcttgaaat ggcatcgggg cagtactatc ccgccgagaa cagactggag ctcttcgact
    19261 acacccatct ggatgttgaa tttcagggcg gaccaggaca ttttgccaca agccacatga
    19321 taagtccctt tgagagcaat tcacgggccc ttatcgagag tgccataaac aaggaggtca
    19381 taaaagaaaa tatcattgaa ggcatacgcc aggatatcat cggtgaagaa ctgctgattc
    19441 tgacccaccc gaatttctat gatgcagcca tcaaactgcg cgactggaaa cgcagcaaag
    19501 gcatctgggc aaatgtgtat gaatgcggaa ccaattccga tatacactgg cgggcaaccg
    19561 gcgagcaaat agacgctttc attgaagagc gctaccacac cacggaaatc agggtaagtt
    19621 atgtgctcct tctgggtgat gccgaattta tccccacctt ctatatcaac aatatcggca
    19681 cagactggcc ctacgccatt ctgggaaagc ccggagagga tctgatcgcc gactttgccg
    19741 tcggccgtat ccccgtcgac acgctcgatc aggcaatgac tgtcgtggac aaaaccatca
    19801 attacgaaaa aacgcccata gacgacaagg acttctatca gaatgccgtt cttgcctctc
    19861 aatttcaatg ctgccgggaa aaagcacccg atcagggcac agatagccgt acctttattc
    19921 aatgctccga gtttgcacag cagatgctct ccgctgcagg caaaacggtt tcacgaatct
    19981 atgcccggac cggaagccag actcccaatc gctattacga cggaaccctg ctgccctctg
    20041 ctctccggcc gtcagccaag tttccctggg atggaaacac aaaccagatc accgaggcct
    20101 ggaacaaggg cgcttttctg atcatccatc gtgatcatgg agagccccat ggctgggaaa
    20161 cgcctcgatt ccgttcaagc cacatcgaca atctggaaaa tgaagacaga ctccctgtcg
    20221 ttttcagcat gaattgctcc acgggtttct ttgacaacga aacagcaggc ggcgcagggg
    20281 gtacggtagc caacgacgtg tacttctgcg agcgggcatt gcggaagccc gatggcggtg
    20341 ctgtcggcat cttcggcgca acacgcataa gtcccagctg ggaaaatacc gctctgacaa
    20401 tgggaatgat ggatgccatc tggccgggcc gcctgaactt cgggttttcc acactgtcgc
    20461 agcggcgtct gggtgatatc ctcaatcatg gcaaacggta catcctttcc atgcgtggcg
    20521 tgtcagtcat gggtgaagat ctctttgaag acagtgtcat agaagaactc tacctctggc
    20581 attgcttcgg cgatcctacg ctggaaatat ggactaaaaa cccgtacagc cagacaaatc
    20641 ccttctcacc ggtcttccat catcagggac ttgctgtgca ggacggcatc gatatttccg
    20701 gtggaatact ggtcgagtac ggcgtcgaca atgcagtcat tactgtcttt gaaagaggcg
    20761 ccgatcagga gatacccctc ggtcgcggtg tcgttcaaaa cggaatcgcc cagatcacct
    20821 acctgcagaa tcatgtgatg gatcatgagc tgaccattat cgccagtttt gataatgccc
    20881 ctgcaaaggt gctgcagggc aactctttct gatgtaaacg gcagacgaaa actgct
//
LOCUS       DAOPWB010000050        20927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2536_length_20927_cov_5.659161, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000050 DAOPWB010000000
VERSION     DAOPWB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 20927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20927
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2536_length_20927_cov_5.659161"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PKN92_06395"
     CDS             <1..1128
                     /locus_tag="PKN92_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48459.1"
                     /translation="RFLDATTSPTRDLYTFNGMNAVTGITPKDSTRENNPYAGCFRGF
                     RVLRYPIAETDRDGKVTRVRRRTDEEMREFGFSVEQYDRMEELVTKGFITEKNIPLTS
                     MHQFIRFRMKAEKPVSPVEIIQRFLTDFAQALSRREEQVRLAWENVQTEGAIEFPGGT
                     ASRNIHHTGGRWGRYASALEDAELRGRYLEFLEEMDAAIQWFDNAPDMLDLTGLNLHA
                     VWTPSDLALAALQKKNQLFAERSIQFTDSHGQPCTLTFLEVEKRLYDLSFNPNLPPEV
                     RWGAAPDTLSPDLLQNCATPLASRKTIALADAYRMERPYRSLTRREIEDTPMSEVADG
                     SHFFSEVLESYLWPKWRGSPSPPLVPHGGKASYERQLASRH"
     gene            1202..2545
                     /locus_tag="PKN92_06400"
     CDS             1202..2545
                     /locus_tag="PKN92_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ48460.1"
                     /translation="MAWNPFAPSPHIERLPEEVIKRKYPLFRWRVMESTFLGYAAFYL
                     VRNNLSVVAPELMETLHYSKSDIGSLLAVTAISYGIGKFVMGAFSDRSNPRTFMALGL
                     LLTAFCNFAFGWVNSFPIHFALWALNGFFQAMGWPPCGRSQGHWFSERERGLMFSIWN
                     TSHNLGGGIAGYVAAWATSYYGGWQYAFYVPGLLALIGAFYIYNRLCDTPQSEGLPPI
                     EEYNNDYPEGMEDHEDRERELTMKELLFHYVLTNKYVWLLAFANFFTYIARYSMLDWG
                     PMYLREIKGASLHSGGLAVLAIEFGGIPSTILMGWLSDLSGGRRGMLSVLCLIPVIGA
                     FTIMLFTPASLVSLDMLMLAVIGLFVYPVINFIVIIALDTLGKKAIGTAAGFIGLFGY
                     LGKTAQAKGFGWILDYAGPAWGEHFAWNLVILAIMTCTLLAGVLLAFTWNLKPRS"
     gene            2663..3136
                     /gene="greA"
                     /locus_tag="PKN92_06405"
     CDS             2663..3136
                     /gene="greA"
                     /locus_tag="PKN92_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNZ48461.1"
                     /translation="MADNTVYITAEGLEKLKADLADLYKRRITVAATIEFARGLGDLS
                     ENAEYHAAKEEQAMIHAYIRDLEDKIARAALLTTSNRDLDKAFLGATVRVNNHKTGKE
                     QVYLLVDPAEADIMSGKISTQSPVGKALLGKAVGDEATAQTPAGPMTLTVLEITY"
     gene            complement(3157..4110)
                     /locus_tag="PKN92_06410"
     CDS             complement(3157..4110)
                     /locus_tag="PKN92_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ48462.1"
                     /translation="MTTARRLIPLVGAFVALMIFFRFLIPGFLNPENMVDLAQQISVN
                     AIIACGMTFCILIGGIDLSVGAVLALVGTASVYVLGLGASETQSLVRVAMALGAGSGV
                     ALVFGAFNGLCASRTKMPPFIITLATMMIARGLALRFNEGRPIPIAPQESIYLGLGNA
                     RIGGVLPVPVLIMVAVVLFSAVLLHRTRFGQHLYGIGGNREAARFTGIAVARTEAAVY
                     MLCAFFTGIAGMIATSQLYSAEPASGAGFELNAIAAAVVGGTSFTGGIGSIPGTFIGA
                     AIIGALDKGLNQAGVHFSFQYILKGLVILSAVYLDVRRKES"
     gene            complement(4107..5624)
                     /locus_tag="PKN92_06415"
     CDS             complement(4107..5624)
                     /locus_tag="PKN92_06415"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNZ48463.1"
                     /translation="MSDDTTTEQSPLLQLEHISKRYGGAVALEQVSVALQAGEIHALL
                     GENGAGKSTLIKILGGIVRADSGTIRMDGQAQAVKNVADADRLGIRIIHQELSLVPAM
                     TVAENIYLGREPRRCGFLSRRQMNQDARQLVERLGLKEIEEVETPLFKMRVAQRQLVE
                     IARALATDARILVLDEPTSALTAGETQRLFLTLRRLRDQGTGIIYISHRLEEIMLLAD
                     RITVLRDGRSVGTRRVCDVSKSELIRWMIGREVADYFQKPVLQESAVVFEARNVKNPH
                     VDGVSFSLKAGEILGIAGLVGSGRSELARALFGMDALEEGELRLNGTVLNLRSPRDAL
                     RAGIVCVPEDRQREGLVMLQTLSFNLALPWTRDWNPHILPDRRKKDAIVHRAMASFAI
                     KAKNPAQNIATLSGGNQQKALVARWMEHRPKVLILDEPARGIDVGAREDIFRILFSLA
                     ESGMSIILISSDLNETLGMSHRIAIYRDRRIVTVLPAAEADQKKIMKLLTGVQVS"
     gene            complement(5621..6595)
                     /locus_tag="PKN92_06420"
     CDS             complement(5621..6595)
                     /locus_tag="PKN92_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNZ48464.1"
                     /translation="MKQISGTLLLLCLFSVLFSGCGSKESNPAGEGGSHSGGRLLGVS
                     FQTMNNPFFVELSAGLKEIIESRGDRLVVLDGQFNSLKQKNDCSDLLMQGAAGIFLNP
                     VNWEGITGTLVQARQQGIPVIVVDAPVRDDSLVLCQVSSDNIEAGRLAARALAEKVPE
                     AKVVILHLSVNKACIDRVAGFQEEMAKHPGMTLLDIQEGKGTIEGARPVMRDLLARYP
                     ELNAVFPINDPSALGAISAIESAGKLGQVTVVTVDGSQEAIAAIRAGKLLSSSAQYPR
                     EIGRVSALKMYEHLEGGTVEKEVKIPVALVTRENVDSFDRAAAPEIHQ"
     gene            6803..8326
                     /locus_tag="PKN92_06425"
     CDS             6803..8326
                     /locus_tag="PKN92_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNZ48465.1"
                     /translation="MLPLQAAFDGKKYLEEQTREIIRRIDRFNNKLYLEFGGKLLFDY
                     HASRVLPGYDPNIKMQLLQQFRDSIDLILCIHAGDIARKKIRADFGITYDMDALKLID
                     DLRLDWGLTVTAVVITRFDDQVSAQTFKNKLERRNIKVYTHRATRGYPTDVDTIVSDD
                     GYGCNSYIETKNPLVVVAGPGPGSGKMGTCLSQLYHEYKRGIQAGYAKFETFPIWNLP
                     LKHPVNLAYEAATADMKDVNVIDPFHLEAYGETTVNYNRDVEAFPLLNRIFHKITGKD
                     SFYRSPTDMGVNRVGFGIIDADAVAEAARQEIIRRVLQTRCDYIQGQSEKVEVERVEL
                     LMNELGITVEKRPVVLPAREAAQQALDANAGHNGVFCGAALQLKDGNITTGKNSPLMH
                     AATSAILNAIKYLAGIPDTVHLLSPSIMDAIGDMKREVKGAQSVSLDLEEALIALGAS
                     APLNGATKVAMEQLKHLRGCNMHLTHIPSPGDEAGLRTLGINVTSDPHFASECLFVP"
     gene            8457..9920
                     /gene="rpoN"
                     /locus_tag="PKN92_06430"
     CDS             8457..9920
                     /gene="rpoN"
                     /locus_tag="PKN92_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNZ48466.1"
                     /translation="MLNISPQIQQRPELRQTLAPQMQQSLAILQMSSLELDQYIDQQV
                     ETNPFLDRTSPREIILSEAVDTLASVDTADYDEPAEGAYEVAVQKKAEESRSADASLR
                     DDYPPDEGYGTHNPDLEARYQYYQDSITRSESLSAHLLNQLHLACSDPDQIAIGERII
                     IGDINKDGLFTGSLKEIAEELNTSVEKVTEVLEIIRKFEPVGVGAASLIDCLAMQIDV
                     EFPDEPLLKQMLLEHWDKLLNRRFALIADALGISEERVQELNRMLARLNPFPGREYSQ
                     EEPVYIYPEITVEEYNGDYIATLTADNLSGLTINEEYIRNIRSQKKSADDEKYVAAYA
                     DSARLLLRNIERREDTIKKVAQAIVDLQRSFMENGPGSMVPLTLEEIAVKVKVHESTV
                     SRAISGKYMETPQGIFEMKYFFSSGLSAAGGEELSSTAVCEQIKAIVEGENKKSPLSD
                     QKITDMLNAQGISVARRTVSKYRELLNILPARFRKVF"
     gene            complement(9944..11287)
                     /locus_tag="PKN92_06435"
     CDS             complement(9944..11287)
                     /locus_tag="PKN92_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48467.1"
                     /translation="MGRTYSIVLCICLLSFALPVFGGSPFSGQEEEWNGFSLVRQEGA
                     DTPSCEIIVPGNEAEGKPWLWCTSSPDEHGALLRSLLEKGFHIVYQKSRSAATFSVCL
                     QQRDVLYNWLTDTASLSKKPLLEASGADALAGFAWAAEHPECMAAIFSVLPLLSPHTS
                     EDAAISEADWEALAQWLEVDSAASFAKTPLACASALGAAKIPMMLVKKLPVSKWTGEQ
                     YDKFYYDYRVAGSGSYDLISCPEKASSEDAGLLFHEQFFFMKYGGLMKETAVGEGVDE
                     ASPWVVADFSSQSGVYMLDDVLVLEKGSDMTGIRWREALPSGGYEITLDAMRLSGSDF
                     FCGLTVPYEESAFSLIVGGWGGTCVGISNLNWQDAYNNETARFKTFNSHEWYSIRLRV
                     DGEHIEAWIDGEQMVDVKVDGRDVDIRWEMMPVKPLGIATWRTMGAIRNFRITPL"
     gene            complement(11336..13825)
                     /locus_tag="PKN92_06440"
     CDS             complement(11336..13825)
                     /locus_tag="PKN92_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNZ48468.1"
                     /translation="MIKRTAVFFLGLAILAGTGYVQEAELSASGKATVTEIGNVPWVP
                     TAEPKVVYGDDDRMDVFQEGDRQKLEWAASTCALVYTTRMTIYADGSADISAPAQYIR
                     SGLPACPDEPFGSQPTASYCTGFMVGDDLIVTAGHCYSSSYFDATRFVFGYDMLDETT
                     PRLNFEANRVYKGVEVVSYSGSGSRDHCVVRVDRRITAPGAKPFPIRRTGTLRVSEPL
                     GIIGHPAGLPKKLAFGNTYVRSIDNPEYFIANVDAFSGNSGSPVINAVTGYIEGILVR
                     GDTDFIYDEVCFRSNKVPNNGGRGEEVTRTLIFADSVPDIENYPGSIRLDARIYKCDA
                     EVTVSVVDKDLRGLPTASVDLEVLSGDREVVVIRPVAGEEDRFTGTIPLRAGEAEADS
                     GFLEVSHGDMIIARYLDETNNEGQTVILETQAAVDCIAPIIQDVSVAYVGSTQARIQF
                     TTDEPSLGIINYGLSCDKLNLQAIGNPAQTVHTVTLSGLSSAKKYYFSIEVLDEASNA
                     SYSDNDGTCHSFQTVALIKTLAEYFNTQNLVDVEYGQATFVPVNTADYYMLCYEDVAD
                     FAVAPAGDPLILGDDTFVEIAMDPGQGITFYGTVYDRFFVGSNGYITFGKGDTNFQAL
                     PSQHFLLPRISGFMADLCPHLRGAVRLNRLSDRYCVSYEDVPVYESGGGYPPENSHTF
                     QIELFFNGIIRLTWKDLFAARAMTGLSPGAGAPPVFTSIKMKNAQGCQELTFSGKPHS
                     ADVNGDWQISPEEMQPVIRYYQQGYHCDSSTADGYAPGAGDATCPPHDSDYLEQDWQI
                     SLSELLRLVQLHNAGGYMPDPLSEDGYAPLP"
     gene            complement(13881..14309)
                     /locus_tag="PKN92_06445"
     CDS             complement(13881..14309)
                     /locus_tag="PKN92_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNZ48469.1"
                     /translation="MTSEKIRQLAQRVPLFHALDGADIEKIVSRGMTMRMLKGETLFY
                     KGTSGNQMYVVLAGKLGVYNGETMIATLRPGDMCGEMALVNHETRSATVKLEEDSLLF
                     VLTETTLNKLLTKRVAVQLLLNIIATLSKRLQEANKRTAP"
     gene            complement(14382..15212)
                     /locus_tag="PKN92_06450"
     CDS             complement(14382..15212)
                     /locus_tag="PKN92_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNZ48470.1"
                     /translation="MLLTDYLSADCVLPEVQTKIKADALKELTDLLIKNRKLKGLEAA
                     LDQIMARESTESTGIGNGIAIPHARISGLKSLYCAMGRFKNGIDFSAVDRKPVYLVFA
                     IFYPPTQQTTYLNFIATLVKMFKNKSHQEIIMKADSAADVFAALKELSLPLEAPEEYY
                     DVKGQTDTALLEARDAHVDLILLARLQLYYEIYETTKANRAKIRERINNIRGLIDEKV
                     LRYFDRLMRARPPAIVPVEADTCQGCFVRLPTQFLQQVRDDTTMIHTCRNCNRFIYIV
                     "
     gene            complement(15336..15545)
                     /locus_tag="PKN92_06455"
     CDS             complement(15336..15545)
                     /locus_tag="PKN92_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48471.1"
                     /translation="MKVQSFMGKVSMTGLRQMDIQINEWLQRSKVKPIHIKQSFGTDI
                     HHEGRMSEPIVIITVWYEDESAVDK"
     gene            complement(15616..16500)
                     /locus_tag="PKN92_06460"
     CDS             complement(15616..16500)
                     /locus_tag="PKN92_06460"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNZ48472.1"
                     /translation="MSAESPLSIRASTQLCAVIGNPVLHSLSPAMHNAAFHAAGLDYA
                     YLAFTVSDLAGCLTGMRALEGFQGLSVTIPHKIAILSYLDEIEESALKIGSVNTVVKK
                     DGKLLGTSTDGAGAFRAIENAGVSLKDASVLFAGTGGAARAVAFALCTSGLPRKIMIV
                     GRSAEKVEALTRDLETVSSSVEVLGGSFSENLLAWAEESDLLINATPLGMHGAAEQEN
                     SFPGVGLRADQAVFDMVYRPENTVLVREAKKQGCKIVPGLDMLLYQAVIQFELWTGAC
                     APEEAMRQALNNALRSRE"
     gene            complement(16530..16877)
                     /locus_tag="PKN92_06465"
     CDS             complement(16530..16877)
                     /locus_tag="PKN92_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNZ48473.1"
                     /translation="MSMETLFTKIISGAIPSDKVYEDDEFCAFKDINPAAPVHVLLVP
                     KKVIPEIGAAAECDEGLLGRLLLTANKVAEQLGIAESGFRYIINSGAHGGQEVPHLHL
                     HIIGGRQLGWPPG"
     gene            17168..17572
                     /locus_tag="PKN92_06470"
     CDS             17168..17572
                     /locus_tag="PKN92_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNZ48474.1"
                     /translation="MQITGKCDYACRAMLELALRYELNTPVSAQLIAEQREIPEKYLT
                     HILLQLKRAKLITSLRGAQGGYMLLRAPDAITLAEIIEAVEGSLSSQYGSKDSDLHIF
                     WQEIAQKTRDVLARYTLASMLDFTLKSNMFHI"
     gene            17588..18844
                     /locus_tag="PKN92_06475"
     CDS             17588..18844
                     /locus_tag="PKN92_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNZ48475.1"
                     /translation="MYISQEKEPKGNKIRKQWTDAVGRGQGTYTPTQAVIEKARGCVL
                     HTADGRRLLDFTSGVLVQNLGYNHPVFEKRFAQYRKNLPRNAYNMITPVQAEASSRLI
                     KSMKSNPKAEKMLWAASGSEGIQKAMWAAMHRYPDRPILVATRFGFHGKKGLAADVTG
                     ETSKNPNVRFVSFPMYTPESPDFYKKELDALYREFPGKIALFITEPYLGAKGSFHPPA
                     WYHQLVQAWCNKHDIPFLFDEVQSCFGRTGNMYAYETYGVTPDMVILGKGLASGESAA
                     AVVGRADLIDSLDYGEASDTYSASVAACSAVCATLDVFKEEQIVRKTKKMAQEVHAML
                     LDLQQTFPFIRDVRGEGMVYGLEIEGDSLANRCVLEAYRATDTRGVHFLGPLAGKVIR
                     VSPPLVLSESELKSAHSLLLKAWKKI"
     gene            complement(18849..20003)
                     /gene="dnaN"
                     /locus_tag="PKN92_06480"
     CDS             complement(18849..20003)
                     /gene="dnaN"
                     /locus_tag="PKN92_06480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNZ48476.1"
                     /translation="MKITIPKNELNNAMNKIKGVAASRTALPVLTNVLLEAEGTRLRL
                     TASDLNISIECTIDCTVIEAGATTAPCQRLSMLLGDLPDADVTLTLHDNGILDVECGL
                     INTKFYTISADEFPPSHDFSSIKPFYLKQAVLKKLFHQTAYAVCNDQSRHNITGLLME
                     TKQDGMHVVATDGKRLSMSVSEDAIPTEEEAEEGSAKAAKFIKDIKTIIPSKTIHELQ
                     SLMTDNIDKSVAVYLGDNKAAFIFENLQVTTALIEGNFPNYTAVIPKKIGKEFILDLD
                     TFRQCMRRCASMTSDKFRTVKFIFHDDNMQIIVKTPEIGEYEENIPVGYQGEKLEISF
                     NPNFILDVLRNINSEQVCLQFNDGNSPGIIKPYTEAPVDSYINVIMPIRA"
     gene            complement(20648..>20927)
                     /locus_tag="PKN92_06485"
     CDS             complement(20648..>20927)
                     /locus_tag="PKN92_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48477.1"
                     /translation="APQAQGPSLTAETLVGTVWEVESPYGPVQVQIQQGGQATVSHPM
                     AGSIPASWKVNGNKVQASASFMGQSINIDATIQGSTLSAKGQKIRRLR"
BASE COUNT         5184 a         5738 c         4975 g         5030 t
ORIGIN      
        1 cgttttctgg atgccaccac ctcgcccacc cgggatctct ataccttcaa cggcatgaat
       61 gcggtcaccg gcattacccc caaagactcc acccgggaaa acaatcctta tgcaggctgc
      121 ttccgcggat tccgcgtgct gcgctatccc atcgctgaaa cggatcggga cggcaaagta
      181 acccgcgtcc ggcggcgcac tgacgaggaa atgcgggaat tcggtttcag cgtggagcag
      241 tatgaccgca tggaagaact ggtcaccaaa ggattcatta ccgaaaaaaa tattcccctg
      301 accagcatgc accagtttat acgcttccgc atgaaagcgg aaaagcccgt gtcgcctgtt
      361 gagattatcc aacggtttct tacagacttt gcgcaggcgc tcagccgtcg ggaagagcag
      421 gtgcgcctcg cctgggaaaa tgtacagacc gaaggcgcca ttgagtttcc gggaggaacc
      481 gcatcacgaa atattcatca tacaggcggt cgctggggac gctatgcttc agctctggaa
      541 gatgcggaac tgcgtgggcg atacctcgag tttctcgagg aaatggatgc cgccattcag
      601 tggttcgata acgcaccgga catgcttgat ctcaccggac tcaacctgca cgctgtctgg
      661 acaccttcgg atcttgctct tgccgcattg cagaagaaaa atcagctttt cgccgagcgc
      721 agcatccagt ttacagacag ccacggacag ccctgcaccc tgacttttct ggaggtggaa
      781 aagcgtcttt acgatctatc tttcaacccc aacctaccgc ccgaagtgcg ctggggtgct
      841 gcaccggaca ccctgtcccc tgatttgctg cagaattgcg caacacctct cgccagccga
      901 aaaacgatcg ccctggccga cgcatacaga atggagcgcc cttaccgaag tttgacccga
      961 cgggaaattg aagatacacc tatgagtgaa gttgccgacg gcagccactt tttttctgaa
     1021 gtgctggaga gttatctctg gcccaaatgg cgcggaagtc catcgccgcc gctggtgccc
     1081 catggcggca aggcatccta tgagcggcag cttgcttccc gtcattagat cacagggaca
     1141 tggtgtgctc ttgctgaagg gcaaaaatgc cctgtccctc taatatataa aggagtctga
     1201 tatggcctgg aacccctttg cgccctctcc gcacattgag cgtctgcccg aagaagtgat
     1261 caagcgcaag tatccgctct ttcggtggcg cgtcatggaa tccacttttc tcggatatgc
     1321 cgccttttat cttgtccgca acaatctgtc tgttgttgct ccggagctga tggaaacgct
     1381 ccattattcc aagtcggata ttggcagcct gctggcggtg acagccattt cgtacggcat
     1441 cggaaaattt gtcatgggag cgttttccga ccgcagcaat ccacgcacct tcatggctct
     1501 cggcctcctt ctgaccgctt tctgcaactt tgccttcggc tgggtcaatt cttttcccat
     1561 tcactttgcg ctctgggcgc tgaacggttt tttccaggcc atgggatggc cgccttgcgg
     1621 acgttctcag ggacattggt tcagcgagcg ggagcgggga cttatgttca gcatctggaa
     1681 cacgtcgcac aatctcggcg gcggtattgc cgggtatgtt gcggcctggg caaccagtta
     1741 ttacggggga tggcagtacg ctttttacgt tcccggattg ctcgcattga tcggcgcctt
     1801 ttatatttac aaccgtcttt gtgatacacc gcaatccgag gggcttccgc ccattgaaga
     1861 atacaacaat gattatccgg agggcatgga agatcatgaa gaccgagagc gtgagctcac
     1921 catgaaggaa ctgctcttcc actatgtgct gacgaataaa tatgtgtggc tcctcgcctt
     1981 tgccaacttc ttcacttata tcgcccgcta cagtatgctc gactggggcc ccatgtacct
     2041 gcgggaaatc aaaggcgctt ccctccacag cggtggtctt gccgtgcttg ccattgaatt
     2101 cggtggcatt ccgtccacca ttctcatggg ctggctgtcc gatctttccg gcggcagacg
     2161 gggaatgctt tccgtccttt gtcttattcc cgtcatcggt gcctttacca tcatgctgtt
     2221 caccccggca tcgcttgtat cactcgatat gcttatgctt gctgttatcg ggctgtttgt
     2281 ctatccggtc atcaatttca ttgtcatcat agcgctcgac actctgggca aaaaagcaat
     2341 cggtaccgcg gcaggcttta tcggactttt cggctacctg ggtaaaacgg cgcaggcaaa
     2401 aggcttcggc tggattcttg attatgcggg ccctgcctgg ggagaacatt ttgcctggaa
     2461 tctggtgatt cttgctatta tgacatgcac gcttcttgcc ggagtgctgc tcgcgttcac
     2521 ctggaatctg aagcctcgtt cgtgatgagg ctcccgcatt tcttgggaaa aaacacagta
     2581 ctttgttata ctccgggttt cccgggtcat cattgtttta tcggatacat gcatctgtat
     2641 ccctggaagg acacctcttt tcatggcaga taatactgtt tatatcactg ctgaaggtct
     2701 ggagaaactg aaggcggatc ttgccgacct ttataaaaga cggatcaccg ttgcggcaac
     2761 catagaattt gcccggggac tgggcgacct cagcgaaaat gcggaatacc acgccgccaa
     2821 agaagagcag gcgatgatac atgcgtacat acgcgatctg gaagataaaa tcgcacgcgc
     2881 cgccctgctc accacatcaa accgtgatct ggacaaggcc tttttagggg caacggtacg
     2941 tgtgaacaac cataaaacgg ggaaagaaca ggtttatctg cttgtggatc ccgccgaagc
     3001 ggatatcatg tccggtaaaa tatccaccca gtcccccgtg ggcaaagcac tgctcggaaa
     3061 agccgtgggc gacgaagcca ccgcccagac acccgccggg cccatgacgc ttaccgtcct
     3121 tgaaatcact tattgactgt tgcttttcac aataaactac gactctttcc ggcgcacatc
     3181 caaatacacg gcagaaagaa tcacaagccc tttcaggatg tactgaaagg aaaaatggac
     3241 acccgcctgg ttcagtcctt tatccagtgc accgataatg gcagctccga taaatgtgcc
     3301 cggtatggaa ccgataccgc ccgtaaaact ggtccctccc acaacggcag cggctatggc
     3361 attcagttcg aaacctgccc cggaagccgg ttccgcacta tacagctgtg acgtggctat
     3421 cattcccgct attccggtaa aaaaagcgca cagcatatag acagccgctt ccgtccgtgc
     3481 caccgcaatg ccggtaaatc gtgcggcttc tctgttcccg ccgataccgt agagatgctg
     3541 tccgaaacga gttctgtgaa gcagcacggc actgaaaagc actacagcca ccatgatcag
     3601 tacaggcacc ggcagaacac cgcctatccg ggcattaccc aatccgaggt aaatggattc
     3661 ctgcggcgct atgggaatag gacgcccctc attaaaccgc agcgccagac cgcgggcaat
     3721 catcatcgtt gccagcgtga taatgaaagg cggcattttc gtgcgtgacg cacacagccc
     3781 gttaaaagcg ccgaagacaa gtgcaacacc ggaaccggca cccaaagcca tcgccacccg
     3841 cacgaggctt tgtgtctccg acgcccccag ccccagcaca taaacagatg ccgtccccac
     3901 aagtgccagc acggcaccta ccgacagatc gatgccgccg ataagaatac agaaggtcat
     3961 cccgcaggca atgatggcat ttaccgatat ctgctgtgcc agatcgacca tattctcagg
     4021 gttaagaaat cccggtataa gaaagcgaaa gaaaatcatg agcgccacaa aggcacccac
     4081 aagcggaatc aaccgccggg ctgtagtcat gatacctgaa ctcctgtcag caacttcata
     4141 atttttttct gatccgcttc tgccgccgga agaaccgtca caatccggcg atctctgtaa
     4201 atggctatcc gatgcgacat gcccagcgtt tcattaaggt cagatgaaat cagaatgatg
     4261 gacatgcccg attctgcaag agagaacagg atgcgaaaaa tatcttcccg ggcacccaca
     4321 tcgatacccc gtgcaggctc gtccagaatc agaaccttcg ggcgatgttc catccatcgg
     4381 gcaaccagtg ccttctgctg attgccgccg gaaagggtgg caatattctg cgccggattc
     4441 ttcgctttga tcgcaaatga tgccattgca cggtgcacaa ttgcatcctt ttttctgcga
     4501 tcgggcaaga tatgaggatt ccagtcacgc gtccagggca gcgccagatt aaaactcagt
     4561 gtctgcagca tgacaagccc ttcccgctgc ctgtcttctg gaacgcatac aatacccgct
     4621 cgcagtgcgt cccggggtga acgcagattg agcacagtgc cgttcagtcg cagttcccct
     4681 tcttccaatg catccatgcc gaaaagtgct cttgccagtt cgcttctgcc cgaaccgacc
     4741 agcccggcaa tacccaatat ttcgcccgcc ttgagggaaa aacttacccc gtcaacatgg
     4801 ggattcttca cattcctcgc ctcaaaaacc acagcgcttt cctgcagcac aggcttctgg
     4861 aaataatcgg caacttctct gccgatcatc cagcgaatca gctcgctttt actgacatca
     4921 caaacccttc tggtgccgac agaacgaccg tcacggagca cggtaatccg gtcggcgagc
     4981 aacataatct cctccagcct gtgtgatatg taaataatgc cggtgccttg atcacggagc
     5041 cggcgcagtg tcagaaaaag cctttgtgtt tctccggcag tcaaagcgga ggtcggctca
     5101 tccagaacca gtatccgcgc atccgttgcc agtgcccggg caatctccac cagctgccgc
     5161 tgggcaaccc gcatcttgaa cagcggcgtt tccacctcct caatctcttt cagcccgagc
     5221 cgttccacca gctgccgcgc gtcctgattc atttgccgcc gggaaagaaa gccacagcgc
     5281 cggggctcac ggcccagata aatattctcc gccacagtca tggcgggcac aagtgatagt
     5341 tcctgatgga tgatacgaat ccccagcctg tctgcgtcag caacattttt caccgcctgc
     5401 gcctgcccgt ccatacggat cgttccgctg tcagcgcgca caataccgcc cagtatcttt
     5461 atcagcgttg acttgcccgc accgttttct cccaggagcg catgaatttc ccctgcctga
     5521 agcgcaacgc tgacctgttc cagcgcaacg gcaccgccat agcgcttgct gatatgctcc
     5581 agctgcagca aggggctctg ctctgttgtg gtgtcgtcgc tcactgatgt atctccgggg
     5641 cggcggcacg gtcaaaacta tccacatttt ctctggtcac aagtgccaca ggaatcttca
     5701 cttccttttc caccgttccg ccttccagat gttcatacat cttaagagca gacacacgcc
     5761 cgatttcccg gggatactgt gccgatgagg aaagcagttt tcctgcacgg atcgcggcaa
     5821 tcgcctcctg tgacccgtca acggtgacca ctgtcacctg ccccagtttt cccgccgatt
     5881 caatggcgga aatcgcaccc agcgcgctcg gatcgttgat aggaaatacg gcattgagct
     5941 ccggataccg tgccagcagg tcgcgcatta caggacgcgc cccttcaatt gtcccttttc
     6001 cttcctgaat atccagaagc gtcatccccg gatgcttcgc catttcctcc tgaaaccctg
     6061 ccacccggtc tatgcaggcc ttattcacgg acaggtgcag aatcactact tttgcctcgg
     6121 ggaccttttc cgccagtgcg cgcgctgcca gccgtcctgc ctctatgttg tcggaagata
     6181 cctggcacaa taccaggctg tcatcacgca caggagcatc caccacaata acgggtatgc
     6241 cctgctgccg cgcctgaacc agcgtgcccg tgatgccttc ccagttgacc gggttcagaa
     6301 aaatgcctgc cgctccctgc atgagcaggt ccgagcaatc attcttctgt ttgaggctgt
     6361 taaactgccc gtccagaacc acaagccggt cccctctgga ctcgataatt tctttcagcc
     6421 cggcacttaa ttcaacaaaa aagggattat tcatcgtctg aaaagagacg cccaggagcc
     6481 gcccgcccga atgcgacccg ccttctcctg ccgggttgct ttcctttgat ccgcatccgg
     6541 aaaaaaggac ggagaagagg caaagcagaa ggagtgtacc ggaaatctgt ttcatgcgtt
     6601 tcatccttat gttggggcag agctgcagaa tatccccgct ccgcatttta acaaatccgc
     6661 accgccctcc ggcaataggc acgcatacgg tctgctgtac cggtaattcg ggcacgccct
     6721 ccagacggaa tatgcccgga attgactggc ccgcatactt ccttatagaa tcctttcagc
     6781 ccctaccgat ctggagacac acatgcttcc cctgcaagcc gcttttgacg gaaaaaaata
     6841 tctggaagaa caaacccggg aaattatcag gcgcattgac cgcttcaaca acaagctgta
     6901 tcttgaattc ggaggaaaac ttcttttcga ttatcatgca tcgcgcgtac ttcccggcta
     6961 tgatcccaat ataaaaatgc agctgctgca gcagtttcgc gacagcatcg atctgattct
     7021 ctgcattcac gctggcgata tcgcccggaa gaaaatccgg gcggacttcg gaatcaccta
     7081 cgacatggat gcgcttaagc tgattgacga tctgcggctc gactgggggc tgacggtgac
     7141 tgccgttgtc atcacacgtt ttgacgatca ggtcagcgca caaactttca aaaataaact
     7201 ggaacgacgc aatatcaaag tgtataccca tcgggctacg cggggttatc ctaccgatgt
     7261 tgacaccatt gtgagcgacg acggctatgg ctgcaacagt tacatcgaaa cgaagaatcc
     7321 tctggtcgtc gtggcgggcc ctggtccggg aagcgggaaa atgggcacct gtctctcgca
     7381 gctgtaccat gaatataaac ggggaattca ggcgggctat gccaaattcg aaacttttcc
     7441 catctggaat ctgcccctga aacatcctgt caatctcgcc tatgaagcgg cgaccgccga
     7501 catgaaggac gtaaatgtca tcgatccttt tcatctggaa gcctacggcg agacaacggt
     7561 aaattataat cgtgatgtgg aggctttccc gctgctcaac cgtattttcc acaaaatcac
     7621 cggtaaggat agtttctacc gctcgcccac ggatatgggc gtgaaccgcg tcggtttcgg
     7681 tattatcgat gccgatgccg tcgcagaagc ggcacgtcag gaaattatcc gtcgcgtact
     7741 tcagacccgc tgtgattata ttcagggtca aagcgaaaaa gtggaagtgg agcgtgtgga
     7801 actgctgatg aatgaactgg gcatcaccgt ggagaaaaga cccgttgttc tgcctgcccg
     7861 ggaagccgct cagcaggcgc tggacgctaa tgccgggcat aatggcgtgt tctgcggggc
     7921 ggcgctgcag ctgaaagacg gaaacatcac caccgggaaa aactcaccgc tcatgcatgc
     7981 cgctaccagt gccatcctta acgcaatcaa atatctcgct ggcatccctg acacggtgca
     8041 tctgctgtca ccaagtatca tggacgccat tggagatatg aagcgggagg taaagggcgc
     8101 gcaaagcgta agtctggacc tggaggaagc gctcattgct ctcggggcat cggctcccct
     8161 caatggtgcc accaaggttg ccatggagca gctgaaacat ctcagaggct gcaacatgca
     8221 tctcacccac attccgtcac cgggcgatga agcaggcctg agaacgctgg ggatcaatgt
     8281 gaccagcgat cctcatttcg cttcggaatg tctgtttgtc ccctgatggc cacggcaccg
     8341 ccaggcaccg tcggcattac ctcctgggca gcgtttcata aaaaataatc ttttccgccg
     8401 atacagagta taataatggt atagaagttt aattgtctta gcggaatttt gttacgatgc
     8461 tcaatatcag ccctcaaatc caacagcgcc ctgaactgcg ccagacactg gcaccgcaaa
     8521 tgcagcagtc tctggctatc ctgcaaatga gcagtttgga gctggatcag tatattgatc
     8581 agcaggtgga aaccaatcct tttctcgatc gcacctctcc ccgggagatt attctgtccg
     8641 aagcggtaga cactctggcg tcagtcgata cggccgacta tgacgaacct gcggaaggcg
     8701 cctatgaggt cgccgtgcag aaaaaggcgg aggagagccg aagcgccgat gcctctctcc
     8761 gtgacgacta cccgccggac gaagggtacg gtacacacaa ccctgatctc gaagcacgct
     8821 atcagtacta tcaggattcc attacccgaa gcgaatcctt aagtgcccat ctgctcaatc
     8881 aattacatct ggcgtgctca gatcccgacc agattgccat cggcgagcgt atcatcatcg
     8941 gcgatatcaa caaggacggg ctcttcacag gcagcctgaa agagatcgcg gaagaattga
     9001 acacttctgt cgaaaaagtg acggaagtgc ttgaaatcat cagaaagttt gagcccgtcg
     9061 gtgtaggtgc cgcctcgctc atcgactgcc tcgccatgca gatcgatgtg gagtttcctg
     9121 atgaaccgct tctcaaacaa atgcttctgg aacattggga taaactgctg aacagaagat
     9181 ttgcgctcat tgccgacgca ctgggtattt ccgaagaacg cgtacaggaa ctgaaccgga
     9241 tgctcgcacg gctcaacccc ttcccgggca gagaatacag tcaggaagag cctgtttata
     9301 tttatcccga aattactgtt gaagaataca acggcgacta catcgcaacc ctgacagcgg
     9361 acaatctttc cggtctcacc attaatgaag agtatatccg gaatattcgc agccagaaaa
     9421 aaagcgctga cgatgaaaag tatgtggcag cgtatgccga ttcggcacgg ctgcttctgc
     9481 gcaatattga acgccgtgaa gacaccatta aaaaagttgc acaggccatt gtggaccttc
     9541 agcggtcttt catggaaaac gggcccggtt ccatggttcc ccttaccctt gaagaaattg
     9601 cggtaaaggt aaaagtccac gagagcacag tttcccgtgc catcagcgga aaatatatgg
     9661 aaacgccgca aggcattttc gagatgaaat actttttttc ttccgggctg tccgccgctg
     9721 gtggagaaga actttcttca acagccgtat gcgaacagat caaagcgatc gttgaagggg
     9781 aaaacaaaaa gtctccgctg agcgaccaga aaatcactga tatgcttaat gcgcagggta
     9841 tttccgtggc acgccgcact gtcagtaaat accgggaact tctgaatatt ctccccgccc
     9901 gtttccgtaa agtcttttag cacccggaat aataacgcac aggttatagc ggggtaatcc
     9961 tgaagttccg gattgcgccc atcgtacgcc aggtggcaat gcccaggggt ttcacgggca
    10021 tcatttccca tcggatatca acgtccctgc cgtccacctt cacatccacc atctgctctc
    10081 cgtctatcca cgcctctata tgctctccgt ccacacgcag gcgaatactg taccattcat
    10141 ggctgttgaa ggtcttgaag cgggctgttt cgttgttata ggcatcctgc cagtttaaat
    10201 tggagatacc cacacaggtg ccgccccagc cgcccacaat cagactgaac gcactttcct
    10261 catagggcac agtcaacccg caaaagaagt ccgaaccgga aagacgcatg gcatccagcg
    10321 taatttcata cccgcccgat ggcagcgctt cgcgccagcg aatgcctgtc atgtcactgc
    10381 ccttttccag cacaagaaca tcgtcaagca tgtacacgcc cgattggctg gagaagtccg
    10441 ccacaaccca gggagaggct tcgtcgaccc cttcaccaac cgctgtttct ttcataaggc
    10501 cgccgtattt cataaaaaag aactgctcat gaaaaagcag accggcatcc tcggaagatg
    10561 ccttttccgg acagctgata agatcatagc tccctgagcc tgccacacgg taatcataat
    10621 aaaatttgtc gtactgctct cccgtccatt tgctcacagg cagttttttg accagcatca
    10681 tgggaatttt tgctgcaccc aaagcggacg cacaggcaag cggcgttttt gcgaaagaag
    10741 cggcgctgtc cacctcaagc cactgcgcca gcgcttccca gtccgcttca cttatcgcag
    10801 catcctccga cgtatgtgga gagagcagag gcaacacgga aaaaatcgct gccatgcatt
    10861 cggggtgttc cgctgcccag gcaaagccgg caagtgcatc tgccccggaa gcttccaata
    10921 aaggtttttt cgataaagaa gccgtgtccg tcagccagtt gtacaggaca tcccgctgct
    10981 gtaagcatac agagaaggta gcggcgctgc gacttttctg ataaacaata tgaaatccct
    11041 tctccagtaa actacggagc aacgccccgt gttcatcagg cgatgaagtg caccacagcc
    11101 acggcttccc ttccgcttcg ttgcccggca caattatttc acaagaaggc gtatccgctc
    11161 cttcctgccg gacgagagaa aagccattcc attcctcctc ctgaccgctg aacgggctgc
    11221 cgccaaaaac cggcaaggca aaagaaagga gacagataca cagaacgata ctatatgtcc
    11281 tgcccatgaa acattcctcc tggcgattgt atggcattac acctgacggg gcaggtcagg
    11341 gcagcggcgc atacccgtcc tccgacagcg gatcaggcat atatcctcct gcattgtgca
    11401 gctgtacgag ccgcagcagt tcagacaggc tgatctgcca gtcctgttca agataatcgc
    11461 tgtcgtgagg cgggcaggtt gcatcgcctg cacctggcgc atacccgtcc gctgtactgc
    11521 tgtcgcaatg atagccctgc tggtaatacc ggatgaccgg ctgcatttct tcgggagaaa
    11581 tctgccagtc cccgttcaca tccgcactgt gcggcttccc ggaaaaggtc agctcctggc
    11641 agccctgagc gtttttcatc ttaatcgaag tgaaaacagg cggtgctcct gcgcccggcg
    11701 ataggccggt catcgctcgc gctgcaaaca aatctttcca cgttagccga atgattccat
    11761 tgaaaaagag ttctatttgg aaggtgtggc tgttttcagg aggatatcca ccaccgctct
    11821 cgtaaacagg gacatcctca taactgacac aataccgatc gcttaaacgg ttcaggcgga
    11881 cagcgccgcg aagatgcgga cacaaatccg ccataaaacc cgaaattctg ggcagaagaa
    11941 aatgctggct cggcaatgcc tgaaaattag tgtcaccctt cccgaaggtg atatagccgt
    12001 tgctgcctac gaaaaaacga tcatagacgg tgccgtaaaa agtaatgccc tgtcccggat
    12061 ccatcgctat ttcgacaaaa gtatcatcgc ccaggataag aggatctcct gcaggcgcca
    12121 ccgcaaagtc agccacgtct tcataacata gcatatagta atctgccgta tttacaggca
    12181 caaaggtcgc ttgtccatac tccacgtcaa caagattctg ggtattgaaa tactccgcca
    12241 gcgtcttaat cagggcaacg gtctgaaaag aatggcaggt gccgtcgttg tcactgtaac
    12301 tggcattgct cgcctcatcc agcacttcaa tactgaaata gtatttcttt gcgctggaca
    12361 gcccgctgag cgtaacggta tggacggttt gagcagggtt tccgatggcc tgcaggttca
    12421 gcttgtcaca ggacagcccg taattaataa tgccaagcga cggctcatcg gtagtaaact
    12481 gaatccgtgc ctgcgtcgac cccacatagg caacagacac atcctgaatg atcggggcga
    12541 tacagtccac agccgcctgc gtttcaagaa taactgtctg cccttcgtta tttgtctcat
    12601 ccagataccg ggcaataatc atgtctccat ggctgacttc caaaaatccg gaatccgctt
    12661 ccgcttctcc ggcgcgcagc ggaatcgttc ctgtaaaccg atcctcttca ccggccaccg
    12721 gccttattac caccacttct ctgtccccgc ttaacacttc aaggtcaaca gacgcggtgg
    12781 gcaacccacg cagatctttg tctacaacgg aaacggtcac ttcggcatca catttgtaaa
    12841 tgcgggcgtc caaccggatt gatcccggat aattttctat atcagggaca ctgtcagcaa
    12901 aaataagcgt tcgcgtcacc tcctcgccac gccccccgtt attgggaacc ttattgctgc
    12961 gaaagcagac ctcgtcataa atgaaatctg tatcgcctct taccagaata ccctcaatat
    13021 accctgtcac cgcattgata accggcgagc cggaattgcc gctgaaggcg tccacattgg
    13081 ctataaaata ttcaggattg tcgatggaac ggacgtaagt attgccaaaa gccagttttt
    13141 ttggaagtcc ggcagggtgc ccgatgatac cgaggggctc ggagacccgc aaggtgccag
    13201 tgcgccggat agggaaaggc tttgcgcctg gagcggtaat ccgccggtcc acacggacaa
    13261 cacagtgatc cctgctgcct gagccggaat agctgacaac ctccacacct ttgtataccc
    13321 tgttggcttc aaagttcagc ctcggtgttg tttcgtcgag catgtcatag ccgaaaacaa
    13381 aacgggttgc atcgaaatag ctcgaactgt agcaatgccc ggcagttaca ataaggtcgt
    13441 cccctaccat aaatcctgtg caataggacg ctgtaggctg actgccgaaa ggctcatccg
    13501 ggcaggcggg cagaccggaa cgaatgtact gagcaggcgc tgaaatatca gcggaaccat
    13561 cggcgtagat ggtcatgcgg gttgtataga caagagcaca ggtggatgcc gcccattcca
    13621 gtttctgtct gtcgccttcc tgaaatacgt ccatgcggtc atcatcgccg tagacaactt
    13681 tgggctctgc cgtgggcacc cagggcacgt tccctatttc ggtcacagta gctttaccgg
    13741 aggctgaaag ttccgcttcc tgcacataac ctgttccggc aagaatagcc agccctaaaa
    13801 aaaagactgc ggtacgcttt atcatgagtg actcccggat cagagcggca ggcagggagg
    13861 gtatagaaaa agcgcgacgg tcagggagcg gtgcgcttat ttgcctcctg cagccgtttg
    13921 ctgagtgtgg caatgatatt cagaagcaat tgaactgcca cacgtttggt aagaagttta
    13981 ttcaacgtgg tttcggtcag tacgaaaagg agactgtctt cttcgagctt tacagttgcg
    14041 cttcgggttt catggtttac cagcgccatt tctccgcaca tatcccccgg acgcaaggta
    14101 gcaatcatcg tttccccgtt atacaccccc agttttccgg caagaacaac atacatctga
    14161 ttgccgctgg ttcctttgta aaagagcgtt tcccctttga gcatcctcat ggtcatgcct
    14221 ctggaaacga ttttttctat atcggcaccg tccagagcat gaaaaagagg aacccgctgt
    14281 gccagctgtc tgattttttc cgacgtcacg agttcttctc cttatcttac gtgcgctatc
    14341 gctgacggac cgcttttctc atgcttgtct ggggcagctg ctcagacaat ataaataaag
    14401 cggttgcaat ttcggcaggt atggatcatt gtggtgtcat cacgcacctg ctgcagaaac
    14461 tgggtcggca gacgcacaaa acatccctga caggtatccg cttccacagg tacgatcgcc
    14521 ggtggtcttg cccgcatcag ccggtcaaaa taccgcagca ctttttcgtc aatgagccct
    14581 ctgatattat tgatgcgttc cctaattttc gccctgttgg ctttggtcgt ttcgtaaatc
    14641 tcatagtaaa gctggagcct tgccagaaga atcagatcta catgggcatc tcttgcttca
    14701 agaagtgccg tgtctgtttg tcccttgaca tcataatatt cttcaggtgc ttccagaggc
    14761 agagaaagct ccttcagtgc cgcaaagaca tctgcggcac tgtccgcttt cattataatc
    14821 tcctgatgcg acttgttttt aaacattttc acgagcgtcg caataaaatt caggtaggta
    14881 gtttgctggg tgggcggata aaaaatcgca aaaacaagat aaacgggctt ccggtcaacg
    14941 gcagaaaaat caattccgtt tttaaaccgc cccatcgcac aataaagaga tttgagaccc
    15001 gaaatacgtg catgaggaat agcgatacca ttgccgatgc cagtgctttc tgtgctctca
    15061 cgggccataa tctgatcgag tgccgcctcc aagcctttca gcttccggtt cttaatcagc
    15121 agatccgtca gctccttcag cgcgtccgct ttgatttttg tctgcacttc cggcaacaca
    15181 caatcggcac tgagataatc tgtaagtaac ataaaaaagc atcctgcatg gctggtccgg
    15241 attgaggttc attccggcac tacaatatca tcagatacta cgtgagtgtg cattgtaaca
    15301 gtttgttttt ttcaattcaa tggatacgac aaaatttatt tatcgacggc tgattcgtct
    15361 tcataccaga ctgtaataat cacgatcggc tcactcatcc gcccctcatg atgaatgtcc
    15421 gttccgaagg actgcttaat gtgaatgggc ttcactttgc ttcgctgaag ccattcattg
    15481 atttgaatgt ccatctgtcg taatccagtc atactgactt tacccataaa actctgaact
    15541 ttcatggcac gccctttctt tcttgaagaa tccggttctg taccgtatta taagcagcaa
    15601 atgccgcaac tgcctttact cgcgggaacg aagtgcattg tttaaggcct ggcgcattgc
    15661 ttcttccgga gcgcaggcgc ctgtccataa ctcaaactgg attaccgcct gatacagcag
    15721 catatccaga cccggcacaa tcttgcaacc ttgtttcttt gcttcccgta ccagcactgt
    15781 gttttccggc cggtacacca tatcgaaaac agcctgatcc gctctcagcc ctacgccagg
    15841 aaaactattt tcctgttctg ccgctccgtg catgcccaga ggcgtggcat taataagcaa
    15901 atcactttcc tcagcccagg ccagcagatt ttcactaaag gaccctccca gcacttctac
    15961 agaagaggat actgtttcta gatcacgggt cagcgcttcg accttttccg cactacgccc
    16021 gacaatcatt attttgcggg gaaggcctga tgtgcataaa gcgaaggcaa cggcacgggc
    16081 tgcaccaccg gttccggcaa aaagcaccga agcatctttc aaagatacac cggcattttc
    16141 aatggcacga aatgcgccgg caccgtccgt gctcgtgccc agcagctttc cgtccttttt
    16201 gactactgta ttgacgctgc caattttcaa cgccgattct tcaatttcat ccaaatatga
    16261 aagaatggca attttatggg gaatcgtgac actcagcccc tgaaatccct ccagagcacg
    16321 catacccgtg agacagcccg ccagatcgga aacggtaaag gcaagataag catagtccag
    16381 gccggctgca tgaaatgccg cattgtgcat tgccggtgat aaagaatgga gcaccggatt
    16441 cccaatcacg gcgcacagtt gagtggaagc cctgatagat aaaggtgatt cggcagacat
    16501 attacaactc ctcctgcctc aaataaatat cagcccgggg gccagcccaa ctgacgcccc
    16561 ccgataatat gcagatgcag gtgcggtact tcctgtccgc catgggcgcc gctgttgata
    16621 atgtaacgga aaccactctc agcaatacct aattgctccg ccactttatt ggcggtaagc
    16681 agtaaacgcc cgagcagccc ttcgtcacat tctgctgcag cgcctatttc cggaattact
    16741 tttttcggaa caagaagcac atgaaccggt gcagcgggat tgatatcctt aaaagcacag
    16801 aattcatcat cttcatatac tttatcggaa ggtatcgcgc cggaaatgat ttttgtaaaa
    16861 agggtttcca tactcatatt gttctccaag atgattactg aaacacaata gcatcctatc
    16921 acaacatatt ccatgaatca aacaggaaat cgcgccaggc aaaaatactg actgttcccg
    16981 taaaaacatg aaaaaacaat tgaaatatgg tactatttct atagttcttt ctttctgttg
    17041 tgtttcagtc gcagaggtgc ttttttcttt taggcacacc gccctgatcg aacttcgcag
    17101 cactttgata cctaaatttt tatttttaaa acagtgctat ttctctagcc aacgtctgta
    17161 gtgtaatatg caaataacag gaaaatgtga ttatgcctgc cgcgccatgc ttgagctggc
    17221 gctgcgctat gagttaaata cgcctgtatc cgctcagctg atagcagagc aacgggaaat
    17281 accggaaaag tatcttaccc atattctgct gcagctgaag cgtgccaaat tgattaccag
    17341 cctcagaggc gctcagggcg gctatatgct tctgcgtgca ccggacgcaa tcaccctcgc
    17401 agaaattatt gaagcagtgg aagggagtct gtcttctcaa tacggttcaa aagacagcga
    17461 cttgcatata ttctggcagg aaatcgcaca gaaaacccgc gatgtactcg cccgttatac
    17521 cctcgcttcc atgcttgatt ttacccttaa aagcaatatg tttcatatct gaccggagat
    17581 acgctgaatg tatatttcac aggaaaaaga acccaaagga aataaaatcc gcaaacaatg
    17641 gaccgatgcc gttggcagag gtcagggtac ttatacacct acgcaggctg ttattgaaaa
    17701 agccagaggc tgcgttcttc atacagctga cggccgccgg cttctggatt ttacctccgg
    17761 cgtcctggtg caaaatctgg gatacaacca tcctgtcttt gaaaagcgtt ttgctcaata
    17821 ccgcaaaaac cttccaagaa acgcttacaa catgatcact cctgtacagg ctgaagcatc
    17881 gtcccgcctg ataaaaagca tgaaatccaa cccgaaagcg gaaaagatgc tttgggcggc
    17941 ctccggttca gaaggtatcc aaaaagccat gtgggcggcg atgcatcgct atcccgaccg
    18001 ccctattctc gttgcaacac gtttcggctt tcatggtaaa aaagggcttg cagccgatgt
    18061 taccggagaa acaagcaaaa acccgaacgt ccgctttgtt tcttttccca tgtacacacc
    18121 ggaatcccct gacttctaca agaaggaact ggatgcgctg taccgcgagt ttccgggaaa
    18181 aatcgcattg ttcattactg aaccctacct gggtgcaaaa ggctctttcc atcctccggc
    18241 ctggtatcac caactggtgc aggcgtggtg caataagcac gatattcctt ttctttttga
    18301 tgaagtgcaa tcgtgtttcg gacgtacagg aaatatgtac gcttacgaga cttacggcgt
    18361 aacccctgat atggtaattc tcggaaaagg acttgccagc ggtgaatcag cggctgctgt
    18421 tgtgggccgt gccgatctga ttgattcact ggattacggc gaagcttccg atacatacag
    18481 cgcttctgtt gctgcctgct ctgctgtctg tgccaccctg gatgttttta aagaagagca
    18541 aattgtcaga aaaacaaaaa aaatggcaca ggaagtacat gccatgctgc ttgaccttca
    18601 gcagaccttc ccctttatcc gggatgtccg tggcgaagga atggtatacg gtcttgaaat
    18661 tgaaggcgat tcactggcaa accgctgcgt gctggaagca tacagagcaa cagatacgcg
    18721 cggcgttcac ttcctcggac ctctagccgg taaagtcatc cgcgtgagcc cgcccctcgt
    18781 cctctcagag agtgaactga aaagcgccca tagccttctt ttaaaagcct ggaaaaaaat
    18841 ctgactgctt atgcgcggat aggcatgata acgttgatgt aactgtcaac aggcgcttcc
    18901 gtatacggtt tgataatgcc cgggctgttg ccgtcgttaa attgcagaca tacctgctca
    18961 ctgttgatat tccggagtac atccagtata aagttcgggt taaaagagat ttccagtttc
    19021 tctccctgat accctaccgg aatgttttcc tcatattcgc caatttctgg cgttttgacg
    19081 ataatctgca tattgtcatc atggaaaata aatttcacag tcctgaactt atcactcgtc
    19141 atggaagcac accggcgcat acactggcgg aatgtatcaa gatcaagaat aaattcttta
    19201 ccgattttct tgggaatcac agccgtataa ttgggaaaat tgccttctat caatgctgtg
    19261 gtaacctgca gattctcaaa gataaatgcc gccttgttat cgcccagata tacagcaact
    19321 gatttgtcga tattgtccgt catcagtgat tgcaactcat gaatcgtttt ggacggaata
    19381 atggttttta tgtctttaat gaatttagcc gcctttgcac tgccttcctc tgcttcctct
    19441 tctgtgggaa tcgcatcctc gcttacagac atgctaaggc gctttccgtc tgttgcaacc
    19501 acatgcatgc catcctgttt ggtttccatc agcagcccgg ttatgttatg acgggattga
    19561 tcattacaca cagcataagc ggtctggtga aaaagttttt tcagaacagc ctgtttcaaa
    19621 taaaagggct taatagaaga gaaatcatga gaaggcggaa attcatctgc cgaaatcgta
    19681 taaaattttg tgttgatcag accacattcc acatccaaga taccgttatc gtgaagggtg
    19741 agtgtgacat cagcatctgg aagatcgcct agcagcatgc tgagacgctg acaaggcgcc
    19801 gtggttgccc cggcttcgat gacagtgcaa tcaatagtac attctatgct gatgttcaaa
    19861 tcagacgctg taagccttaa acgggtgcct tctgcctcca gcagtacatt ggtcagaaca
    19921 ggcaatgctg ttctggatgc tgcaacccct tttatcttgt tcattgcatt gttaagttca
    19981 ttttttggta tcgtgatttt catgagatgt ttcccggttc ctgtccgttg tataacttat
    20041 gcatctttat acagatttct ttgtatataa agcaagtcgt agtcttaata gggcgtgtgg
    20101 aaatgtggac aacccatttt caagcctgta aagaggagct attcagaaag aataacttct
    20161 ggataatctg ttgtatttat ccactgtcat ccacaatgtc acaaaaaaaa taaaaaccga
    20221 aaaaagaaat tttgtttttt tgtggataac tcttttttta tacacaaaaa cggggggact
    20281 tatccacaac gccccgcatg gcaatgttcc acgctctttt ttaaaagtta tccactgtaa
    20341 aaaaccttga tatataacgc tgaacagtct gtttaacgaa ggttatgcac aggtcgtgga
    20401 acatggggag ccccataaaa tttttgtaat ctgcacccgt atctgttcct acaacttctt
    20461 ttatatcaat aacttacaaa ttcaataaat acacttaaaa aagcatttca gactgtaata
    20521 taaagggtat tcttattttt ttagaaataa tgtgccactc agccgggtgt gaattctgtt
    20581 tctgcggaaa agttacacac aacttatcca cattaatttt attgagcggt acgtattcgg
    20641 ggccggatca gcgaaggcgc cgtatctttt gccctttggc ggacaaagta ctgccctgaa
    20701 tggtagcatc aatattgatg gactgcccca tgaatgaagc ggaagcctgt actttgttgc
    20761 cgttgacctt ccagctggcg ggtatacttc cagccatggg gtggctgact gttgcctgac
    20821 ccccctgctg tatctgcacc tgtacggggc cgtacggcga ttcgacttcc cacacggtac
    20881 cgaccagggt ctcggctgtt aaagagggtc cttgcgcctg tggagcg
//
LOCUS       DAOPWB010000051        20552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2608_length_20552_cov_6.251500, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000051 DAOPWB010000000
VERSION     DAOPWB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 20552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20552
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2608_length_20552_cov_6.251500"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKN92_06490"
     CDS             complement(<1..683)
                     /locus_tag="PKN92_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48478.1"
                     /translation="MRRFYGLLFFLLIPALLPATADAEDPNPATLHSSRGAWPLYRQW
                     TPAETRHFAQWISTIYDRKVHGTKEQRLAKLEYVLTDPEMNLLLDPEFLGEPSNPQID
                     LPTIRALHHIVDCHKLLMSLGSYYACRRGLPFMYSTVRSSDGTDIRTAPSTLPAGTAS
                     SFEYSSAHQFFVDLVTGTCTGNLRVEPFARNSEWSDTCPVAITPQYLIPGCSFYLDGH
                     VLVLAEIEEG"
     gene            complement(786..2564)
                     /locus_tag="PKN92_06495"
     CDS             complement(786..2564)
                     /locus_tag="PKN92_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48479.1"
                     /translation="MPFFPYFNRPLTLLLLGAVSLTALLAAAADPNPPSIRGGGAWPI
                     RRQWTPAETMHYARWVEHLFDKKTTGTVEQRTAKLEYLLTDPDMNLLQDPSFLGEGGN
                     PQMPAGLIRSMHHLMDCGKFTAFLPAYYAYRRALPWITATVSSGQRGVDVRISDFNIP
                     YGGTNSFTSPSLSQFFNAAVGHFISGNYRVNLNGRNAEQSDTVPVALNRDKLLPGCMN
                     YLDGHCLVLAKVTEYGELYFLNCSTTTSRDIFTYNGMNVVGGMTPRGSDPDDEWAGCF
                     QGLRVLRYPIAETDGRGNVTRVRRRTDAEMREFGFSTEQYDLTREMYDNHFIEEGGLR
                     PSSIHDLIRLRMKTLDRIAPAAFIRQYCDELLQAYLERERFIQDAWKDVLRNGPIVYP
                     EDRDKDNIFQATGRWETWSSPSSDVDRRNKYFYLADWMEYAIRMFEMKPDFIDMTGLE
                     SYGIRSQADLAAAMIAEKNQHFDRLSLDYTNSKGKTVTLTLKDIEERLYDLSFDPNHP
                     PELRWGAPFDSDEFAGAPEPPTPLPDGFKMPMKEAYRLQAFYRSLCQRETTTSYLRGM
                     FTTGYPIRDKLDAQVGKWSYATSPLL"
     gene            complement(2797..4323)
                     /locus_tag="PKN92_06500"
     CDS             complement(2797..4323)
                     /locus_tag="PKN92_06500"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HNZ48480.1"
                     /translation="MTIPELLEKSVRQAPDLPMIIFEKEEVTYRQMLERSCLVAGNLK
                     AQGIQPGDKIALMMGNCLEYLYCFMGCGRIGALLVPINPALSFEEFAYIIQNSEARFL
                     IIMAQFRPVLPRLFEALPNLEKVFILGESCEHSSSFSELLQPVETPDIVTTEASDAAL
                     IYTSGTTGKPKGVILTHGNYAWDTLSLLRSNRITSRDRFLCVLPLFHVNAQVVSILTP
                     TLCNATIILVGGTFTPYTILPLIEQHKITIMSAVPTIYGLMTRLPKAETTDVSSIRFF
                     VSGAAPMPEAIYEAVQRVFKKPLIMGYGLSEATCASAVADHEDPIRWNSVGPALRYTS
                     IRIVDKKGHDVPVGEVGEVLISGPTVMKGYYRNPEATAEVLQNGWLRTGDLGHFDAEG
                     YLYIVDRVKDMIIRGGLNIYTAQVEQIIARMAAVEEVAVIGVDEPTWGQEVLAVVQVK
                     SGDTLTEKAVIDFCKENLAAFKCPRYVRFVDQLPKTAIGKVRKHELIDQFGNVALRKK
                     "
     gene            complement(4517..5743)
                     /locus_tag="PKN92_06505"
     CDS             complement(4517..5743)
                     /locus_tag="PKN92_06505"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNZ48481.1"
                     /translation="MHVTAYLRGTLSLLTLACFVACPAARGADVAAPPGIVISHSPQS
                     TGKYIGSPSITVLPSGHYLAAHDYFGPESNEHVCATAVVFRSEDRGESWTRLAEMQCL
                     FWPKLFTHRGAVYLLAVEKHHGAIVIRRSDDEGAHWTEPLDAHSGRLTPSEEQYHTAP
                     GPVIEYKGRLWRAFEDAMGGIRWGERYQALMMSAPVDADLLNASSWTFSNPLPRDPLW
                     LDGTFGGWLEGNAVVDPSGAIVNVLRVDTPGYPEKAALVTVSEDGSAVSFDPCSGFID
                     FPGGAKKFTIRHDPESGFYWTLSSIVQEQYVGTERPARIRNTLALMRSPDLRQWEVRA
                     IILHHPEVEHHGFQYVDWQFEGNNLIAVCRTAYDDDEGGARNNHDANFMTFHRIMDFR
                     TLTTADPLCADRISDS"
     gene            complement(5754..6230)
                     /locus_tag="PKN92_06510"
     CDS             complement(5754..6230)
                     /locus_tag="PKN92_06510"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48482.1"
                     /translation="MSKSFFSGAWALISDLALTAWRFVSFRSSTAEFTHDFWDSISAS
                     WQEGSSSAVTGERKKARKYLQQGVKHYNAKRYSDALYSFQKALQEDPHYSRGLLYYGN
                     ALYKLHEDTDAVKSWQQAIAVEPRSASARAAQMKLAHIQDKTKLASDEIQSQLNHK"
     gene            6403..7668
                     /locus_tag="PKN92_06515"
     CDS             6403..7668
                     /locus_tag="PKN92_06515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ48483.1"
                     /translation="MTTPNGTTPLNGRIAFLDFLFCWPPKGGADVDVYSVMTGLHRRG
                     CQVKLFVPAFAGCPGRGEVEADVLSFPVEIVPVPRKRQQLPALAEQLQKALDGWQPDI
                     VIVCHGYLLKTALIPMLKAKTLISRIYANEIFCLKDAFHYKDGAPCPLYLHKEPELCR
                     KCAAEHLETQVKKGLWDPWTHEYIITRGWDSSFGTAHRNALDRLDSIIVYNESIKGAL
                     SPWDSKVTVIPGGITAEPVVSASAHPEKQGQGMRVLMAGRVEDPLKGLAVLEEACCAL
                     RRKGYVFEVQVTHYDPFFGRPELKATGWLSHEETLALYRNAHIAVVPSLWEEAFGLVA
                     VEAMAAGVPVCASRTGGLQEIIEEGKTGFLFKPGDSAALADVLEKLLQDPELRKRMGD
                     AAQQRALEEYAWDKVIEKHYIPLLKGFLP"
     gene            7665..8162
                     /locus_tag="PKN92_06520"
     CDS             7665..8162
                     /locus_tag="PKN92_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48484.1"
                     /translation="MKKRFAVLYSLAPHFRRSLLALREEEPDSHITLLLPPGFPQEVV
                     EGCCDEILALAPASSQRRSLKHAWRLIRYLRRERFDCFIVFFNSPALWLLTQCSGARE
                     CAYFSQRGARCPMSMSFLRSVCALAGKRIRGRYMYYKIKWDIYRHPFSDTSRAADRKE
                     QDGDS"
     gene            8183..8815
                     /locus_tag="PKN92_06525"
     CDS             8183..8815
                     /locus_tag="PKN92_06525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ48485.1"
                     /translation="MAGKQVEQALALLEGLNLPCFCRDGLQKAEEHIRLIREWNHAIS
                     LVSQGDLAFLPERHLVDALSLLAVIMQTSGEKRLLDIGSGAGYPAIPMKCFCPTLDVT
                     LVERSRRKAGFLQRVISLLGLEGVTVLTGEFPAQPERLDAQWITARAVERPKKVYADV
                     LQRLPQGSVFLCQLEGVPPVASEMFHVEHVEDVWQSSGLRRGSLRLIRKK"
     gene            complement(8863..9462)
                     /locus_tag="PKN92_06530"
     CDS             complement(8863..9462)
                     /locus_tag="PKN92_06530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ48486.1"
                     /translation="MCLKPIIKAGGCLVFIVIPLVIFAIYWANREDDVVFPDPENSPY
                     CLPWEAGKSYWCIQGVGGIVSHHGSSRYAYDFYMPEGTEVRAAREGKVVKVVQEHTDH
                     GYKKPNNLVAIEHSDGTWAYYGHIQQQGSKVAVGDKVKQGQAIALSGHVGHSLVPHLH
                     FQVMDGKTRTTLPASFADVTRDGGVPRMFRRYTSRNEPK"
     gene            9689..11218
                     /locus_tag="PKN92_06535"
     CDS             9689..11218
                     /locus_tag="PKN92_06535"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNZ48487.1"
                     /translation="MAKHDLNRREWLQRSTAAVLGGAYALQAQSAAPLRPNILLLMAD
                     QMRGDCLGTAGHPAVLTPALDRLAAEGVLFRRAYSSVPSCTPARAALLTGLGPWKNGM
                     LGYSRIPEQYAREKPRMLREGGYSTCSIGKCHYHPQRNGHGYEKLLLDESGRVEAPDF
                     ESDYRRWFRMTAPDLNPDATGLGWNDYPARPYALPEELHPTCWTGNEAVRYLENYRED
                     APFFLKVSFARPHSPYDPPQRFFDLYADREIPAPVIGDWCARYAPRASEKDSLWHGDL
                     GTDTVRHSRRGYYGSITFIDEQIARILSALEKSGRMENTLILFTADHGDMLGDHHLWR
                     KTYGYEGSAHIPMILRAPASFDVPCKGVVRDEVVELRDILPTFLDAAGLGGAEEMDGR
                     SLLQLLRDDGRSWRPWLDLEHSRCYVDSEQWNGLTDGKSKYLFFGQSGEEQLFDLTAD
                     PGECVNLAGTPGAASLLELWRRRLADHLQERGADWVPDGTLKRRPKDVLHSPNYRQPE
                     G"
     gene            11330..12325
                     /locus_tag="PKN92_06540"
     CDS             11330..12325
                     /locus_tag="PKN92_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNZ48488.1"
                     /translation="MLYNTLGGSDLQVSALSLGVWQLGDPGFWGEEACSSPADLVAAA
                     IDHGITLFDTAELYGNGSSEELLGAALKGRRDEVLLASKVSTQHCTAEGIRSACENSL
                     SRLQTDRIDLYQIHWPFPPADYAAVQETLEALRQEGKIREIGVSNFGRRDLQGWMAEG
                     TAVSNQIGYNMLFRAPEYEMIPACRKHNLGVLAYMPLMQGLLTGRFKSTEEIPFQRRR
                     TRHFSCTREGTRHGQVGHERLLWDTLEDLSDFADAIGVPLSVLSLCWLMRQPGVSSVI
                     IGSRKVDQLLFNLQAAQLDIGPAATAQLNEATYPLKVALGRNCDMWESDDNRRIN"
     gene            12403..13374
                     /locus_tag="PKN92_06545"
     CDS             12403..13374
                     /locus_tag="PKN92_06545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ48489.1"
                     /translation="MRITFVIPAYNEAPTLAALAEGIKAHVGAHDFRILFVDDGSTDN
                     SWEILEGLRREDARIDLLRLRRNCGKTAALEAAFALVSGDVLITMDADLQDDPAEIPR
                     MLAKLDEGYDLVCGWKKKRHDPWLKTIPSRFYNARISRRFKLPLHDINTGFKAMRMEV
                     AKRLRLWSDMHRLIPVFAAHLGYKVTEIPVRHHPRKFGKSKYGFTRIQKGLVDIESAA
                     YILKYQDAPMRRIYISQRGLLYVLLLVLLTLSLALIVQEVPEALILLLFSSLAIALYF
                     LTGCFFRLAQMHQLILYRMPPPDPGRFVAAYHATPWADDAESPGQEN"
     gene            13408..13893
                     /gene="bcp"
                     /locus_tag="PKN92_06550"
     CDS             13408..13893
                     /gene="bcp"
                     /locus_tag="PKN92_06550"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNZ48490.1"
                     /translation="MTKKADNHPLLGKKAPAFTLESSDRKKVQLSNFKEGLVVLYFYP
                     KNNTPGCTLEAQEFRDLHDAFTKLNVTVLGVSPDSPSSDCRFSEKLSLNFLLLSDRDH
                     AIAEKYGVWVEKSMCGKKYMGVERTTFLIDGSGKIIHVWGKVTAKGHALAVLDLIKNR
                     A"
     gene            13953..15740
                     /locus_tag="PKN92_06555"
     CDS             13953..15740
                     /locus_tag="PKN92_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNZ48491.1"
                     /translation="MVVSTLDWVIVGFFFLFYLGVGLILTRRAGSSTAEFFVAGRSMP
                     WWLLGVSMVATTFSTDTPNLVANIVREDGVMGNWVWWAFLPTGMLTVFVYARLWRRSG
                     LLTDLEFYELRYSGKSAAFLRGFRAIYLGVFFNVIVMASVSLAAIKIGSVLLNLTPMQ
                     TILIASTITVIYSSSGGLRGVLFTDLIQFTVAMIGAVAAAVIALRHPDVGGLSKLLAH
                     PALEGKLSIFPSFTDPDQRGVLISVFLMPIIVQWWSVWYPGSEPGGGSYIAQRMLAAK
                     DEKNAVGAVFLFNAAHYALRPWPWIVVALCSLLVFPSLDSLREAFPHAAAIIGHDMGY
                     SAMLTFLPAGVLGLVIASLIAAYMSTISTHLNWGASYAVHDFYSRFLRPDASEKELVL
                     VGRVVTVVLMILAGGVALIMESARDNFQIMLMVGAGTGLIFILRWFWWRINAASEIAA
                     MIISFFVALYFRFLHHRFGFLPLSDATQLLLTVGITTAGWLAVTWLTRPVDEEVLQRF
                     CSLIRPGGPGWKGIPIDPQSVPHGDTAWQVPRGILCMLLGCLSIYSALFATGYLIYGN
                     YLPGVLLSATAVISGYVLVRQWRVLLARD"
     gene            complement(15752..16405)
                     /locus_tag="PKN92_06560"
     CDS             complement(15752..16405)
                     /locus_tag="PKN92_06560"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNZ48492.1"
                     /translation="MHEPDYDSLRKIMVEEQLRKRGITDPGVLAAFLSVPRHLFVDAD
                     WKTQAYEDRPLPIGAEQTISQPYMAALMTQWLCPSPEHRLLEIGTGSGYQAAVLSLLA
                     HTVFTVERIDVLYHNASKRLTELGYRGITPLLGDGSTGHESGALYDGILVTAGAPEIP
                     EALLAQLKEGGMLVAPVGDRQHQRLIRIRRHRNGFHREEGTPCRFVPLLGAQGWQQP"
     gene            16745..18547
                     /locus_tag="PKN92_06565"
     CDS             16745..18547
                     /locus_tag="PKN92_06565"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNZ48493.1"
                     /translation="MKNCDQVLTTNSIVTHWIEEMAALCLPDKIVWIDGSDEQRQELI
                     AEACRTGEIIALNQEQYPNSYYHRTHPLDVARVEDKTFICCQRERDAGATNNWKNPEE
                     AYTEAANFFRGGMRGRVMYVVPFSMGPVGSPFSKIGIELTDSIYVVLNMMIMCRVGKQ
                     VLDVLGHSDDFTRCLHSKATLDPEKRRILHFPEDNTIWSVNSGYGGNVLLGKKCLALR
                     MASWMARKEGWLAEHMLILGVEEPSGRVDYVAAAFPSACGKTNLAMLIPPEGLKSKGY
                     RIWTVGDDIAWIRPDTDGRLWAINPEAGFFGVAPGTNSSSNRNALLTVRKNTIFTNVV
                     LGEDGTVWWEDGEGDPPANATDWLGNPWYPGKKDENGKEILGAHPNSRFTAPLNQCPS
                     ASHRMEHHHGVPISAFIFGGRRNHLAPLVYEAYSWNHGVFVGASMASERTSAQYGKQG
                     EVRRDPFAMLPFCGYNVADYFKHWISIGRSIKHPPKIFHVNWFRRDENNKFLWPGYGE
                     NLRVLEWILQRARGEGDAVETPIGYIPGKESLDMTGLELAPETLDELLTVNREDWKAE
                     ADDIEQFFDSLGLKVPWELRNELEALRNRLSDDE"
     gene            complement(18610..19935)
                     /locus_tag="PKN92_06570"
     CDS             complement(18610..19935)
                     /locus_tag="PKN92_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNZ48494.1"
                     /translation="MNTSIGMPLRLIFFMIIAAVCSATELKDWESRAPRKEIAPSFER
                     LPDGRLRISADDREGLDGCWFKSFPVTGGQWYRFDARYQASGVEYPRRSVLARLLWQS
                     GDGKALYHGEPGAHSYQGGRNPRSEPEYPVCFGPDEDGWSHVSGTYQSPDGAAAVQVE
                     LYLRWARKASVEWRDIRLTPVEAPPPRRVRLGAVHYVPRNAASGLDSCRQFEPLIRQA
                     SEAKVDLLVLPESIPTTGTGKPHHEMAETIPGTMSDYFAEMAEKYHLYIVAGLTERAG
                     SLIYNTAILADPAGALVGKYRKASLPRNEIEAGITPGTDYPVFSTPIGRIGIMICYDG
                     FFPEPARQLALNGAEIIAFPVAGCNPLLAAARACENHVYLAASSYTDISMHWMITGIY
                     DQEGRVLAQAMEWGSIAVAEVDLSRRLYWESLGDFKGEMPRHSPAGHNN"
     gene            complement(19963..>20552)
                     /locus_tag="PKN92_06575"
     CDS             complement(19963..>20552)
                     /locus_tag="PKN92_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ48495.1"
                     /translation="LYDDALIFLTADHGEEFWEHGHFEHGHNLYNPLVQVPLLLKQPK
                     SIKTGIVEVPVTTLGIMPTLMESCGQAVPSYLAPNTFPVSLHNEQPGESAPPVFIGTN
                     IFYDPLESVVFRNKKYIRSLTSSAEELFDLSIDPAEKHSIATDVPELVAEAQALLKKA
                     EEEDAALRQHFGIGDESDYSINLEDVRSLEALGYL"
BASE COUNT         4532 a         5280 c         5875 g         4865 t
ORIGIN      
        1 ccttcttcaa tctctgccag taccagcaca tgcccgtcca gataaaagga gcatccgggg
       61 atcaggtatt ggggcgtgat ggcgaccggg caggtgtcgc tccattcgga attgcgtgcg
      121 aaaggttcga cgcggaggtt gcctgtacag gtgcctgtga ccaggtcaac aaaaaattga
      181 tgcgcggaac tgtattcaaa ggagctggcg gtgccggcgg gcagggtgga tggcgcagtg
      241 cggatgtccg ttccgtctga ggagcgcacg gtgctgtaca taaaggggag gccccgtcgg
      301 catgcgtaat aacttcccag agacatgagc agtttatgac agtcgacaat atgatgcagc
      361 gcccggatgg tgggcagatc aatctgcgga ttggagggct cacccagaaa ttccggatcc
      421 agaagcagat tcatttccgg atcagtaagc acatactcca gttttgccag ccgctgctct
      481 tttgttccat gcacttttct gtcgtatata gtggaaatcc attgtgcaaa atggcgggtt
      541 tccgccggtg tccactgtct gtacagcggc caggcacctc tggaagaatg cagcgttgcc
      601 gggttaggat cttctgcatc ggccgttgcc gggagaaggg ctggaataag aagaaagaaa
      661 aggagtccgt aaaatcggcg catagggcat cctttcaaaa taaagaacaa tggacggtgc
      721 aggcgcggca aggagggccg gtatcttcgc cttcggatat cagtttcatg agagaaccgg
      781 cacacttaca ataaaggact ggtggcataa gaccatttgc ctacctgcgc atccagttta
      841 tcacggatcg ggtagcctgt tgtaaacata ccgcgcagat aactggttgt cgtttcacgc
      901 tggcagaggc ttcggtaaaa agcctggagc cggtatgcct ctttcatagg cattttaaat
      961 ccgtcaggca gcggtgtagg cggttccggg gcgccggcaa actcgtcact gtcgaaaggc
     1021 gcaccccagc gcagttccgg cgggtggttg ggatcaaaag aaaggtcgta gagccgctct
     1081 tctatatcct tcaaggtcag ggtgactgtt tttcctttcg aattagtgta gtctaaagaa
     1141 agtctgtcga agtgctggtt tttttcggca atcatggcgg cagcaagatc cgcctggctt
     1201 cggatgccgt aggattcgag ccctgtcatg tcgatgaaat ccggtttcat ttcaaacatg
     1261 cgtattgcgt attccatcca gtcggcaagg tagaaatatt tgttgcgtcg atccacatcg
     1321 gaggaagggg aactccaggt ttcccagcga ccggtcgcct ggaaaatatt gtccttgtcg
     1381 cggtcttccg ggtacacaat aggtccgttg cgcaggacat ctttccaggc atcctgaata
     1441 aaacgttccc gctccagata agcctgcagc agctcatcgc agtactggcg gataaaagcc
     1501 gcgggtgcaa tgcggtccag cgttttcatc cgcagccgga tcagatcatg gatgcttgaa
     1561 gggcgcagcc cgccttcctc aatgaaatgg ttgtcataca tctcacgggt gagatcgtac
     1621 tgttccgtgc tgaatccgaa ttcccgcatt tcggcatcgg tccggcggcg aacccgggtg
     1681 acattgcccc ggccgtctgt ttccgcaatg ggataacgga gaacccgcag accctgaaag
     1741 cagcccgccc actcgtcgtc cgggtcagac ccgcgcgggg tcatgccgcc tacaacattc
     1801 atgccgttgt aggtaaagat atcgcgggag gtggtggtgc tgcagttaag gaaatagagt
     1861 tcaccgtact cggtaacttt cgcgaggaca aggcaatgtc cgtccagata gttcatacag
     1921 ccgggcagta atttatcccg gttcagcgcc accggcaccg tgtcggactg ttcggcattt
     1981 ctgccgttca ggttgactct gtaatttccg gaaataaaat gacccactgc tgcattaaaa
     2041 aactggctga gcgaagggct ggtaaaacta ttggtgccgc catagggtat attgaaatcg
     2101 gaaatgcgga cgtccactcc gcgctgtccg gaactgacgg tggcagtaat ccagggaagg
     2161 gcgcggcggt atgcgtaata ggcaggcaga aaggcggtga attttccgca gtccataaga
     2221 tgatgcatgg agcggattag cccggcgggc atctgcggat tgccgccttc cccgagaaag
     2281 gaaggatcct gcagcagatt catgtccgga tcggtaagaa gatattccag tttcgctgtt
     2341 cgttgctcaa cggtacctgt tgtctttttg tcgaaaaggt gttccaccca gcgcgcataa
     2401 tgcatcgttt cagcgggcgt ccactggcgg cggatgggcc aggcgccccc gccacggata
     2461 gatggcgggt tgggatcggc agcggcagca agcaaagcgg tgagcgacac ggcgcccaga
     2521 agaagcagcg tcaatggtcg attaaaatac ggaaaaaatg gcattgcatt gaaccctgaa
     2581 aaaatataat gatgtgaaag aaaaggaaag cgaggtgtgg agcagcgccc ctgtcctctg
     2641 cggttattat agacagaaca gcggacgcat ccaaaaagag tgccgtgacg actgtgcccc
     2701 gtctgaacaa aaaagacctg cgtagagcag aaatggcgtc tgcgagtttc tccgggccgc
     2761 gtggtgtgtt cttaaaggcg ggcggatcag gaggctttat tttttgcgta aagccacatt
     2821 gccgaattgg tcgatgagtt cgtgcttccg gacttttccg atggctgttt tcggcagctg
     2881 atcaacaaag cgaacgtaac gggggcattt aaaagcggca agattttctt tgcagaagtc
     2941 aatgactgcc ttttcagtaa gcgtatctcc gctttttacc tgaaccacgg caagcacttc
     3001 ctgaccccag gtgggttcat caactccgat gactgccact tcttccaccg ccgccatccg
     3061 ggcaataatc tgctcgactt gagcggtata gatattcagg ccgccccgga tgatcatgtc
     3121 tttgacacgg tcaacgatat aaaggtagcc ttcggcatcg aaatgaccga ggtctcctgt
     3181 tctgagccat ccattctgga ggacttcagc ggttgcctcg gggttgcggt agtagccttt
     3241 catgacagta ggaccggaga taagcacttc gccgacttcg cccacaggca cgtcatgacc
     3301 ctttttgtcc acgatgcgta tggaagtata gcgcaatgcg gggccgacag aattccagcg
     3361 gatggggtct tcgtggtctg caacagcact ggcgcaggtt gcttcgctta agccgtaacc
     3421 catgatcagc ggctttttaa atacgcgctg gactgcttcg tagattgctt cgggcatagg
     3481 agcggcaccg gaaacaaaga agcggatcga acttacatcg gtggtctccg ctttaggaag
     3541 gcgcgtcatc aagccgtaaa tagtgggcac ggcgctcatg atggttatct tatgctgttc
     3601 aatcagaggc agaatagtat aaggtgtgaa agtgccgcca acgagaatga tagtcgcatt
     3661 gcagagggta ggggtcagga tggaaacaac ctgcgcattg acatgaaaca gtggcagcac
     3721 acagaggaaa cggtcgcggg aagtgatgcg gttcgagcgc agcagtgaca gggtgtccca
     3781 ggcataattg ccgtgggtga ggataacccc cttaggtttg ccggttgtgc ccgatgtata
     3841 gatcaatgcg gcatcggaag cctccgttgt cacaatatcc ggggtctcga cgggttgaag
     3901 cagctcggaa aaagaagagg aatgttcgca ggactcgccc aggataaata ctttttccag
     3961 attcggaagg gcttcaaaaa ggcggggcag gacgggtcgg aactgtgcca taataatcag
     4021 gaaacgagct tcggagtttt gaataatata agcgaactcc tcaaaactca atgccgggtt
     4081 gatgggaact agcaatgcgc cgatacggcc gcagcccata aaacagtaaa ggtactccag
     4141 gcagttgccc atcatgagcg caatcttgtc gcccggctga atgccctgtg ccttcagatt
     4201 gccggcaacc agacaggagc gttccagcat ctgccggtat gtcacttcct ctttctcgaa
     4261 aatgatcatg ggaagatccg gcgcctgacg tacagatttt tccagtaatt ccggtatagt
     4321 cattttgtct ttcctgctga cggacacgga ggtgatccga cctgcttcca tccggagggg
     4381 tggtcggtaa tgggagatag tatacccgaa ggctgtatgc cagccaaaat ctttacaagt
     4441 tctaacaagt aaacggttac catgatgggc acagcataaa ataagttgcc ataaaaaaga
     4501 aaaaacgcac aaagatctag gagtcggata tcctgtccgc acaaagcgga tccgcagttg
     4561 taagggtgcg gaaatccata atacgatgaa aagtcatgaa gttggcgtca tggttgttgc
     4621 gggcgccgcc ttcgtcgtcg tcatacgccg tgcggcagac cgcgatcagg ttgtttcctt
     4681 caaactgcca gtccacatac tgaaaaccgt gatgctccac ttcgggatga tgcagaatga
     4741 tcgcacggac ttcccactgt cgcaagtccg gggagcgcat gagcgccagg gtattcctta
     4801 tgcgtgccgg gcgctcagta ccgacatact gttcctgtac gatggaggat agtgtccagt
     4861 agaatccgct ttccggatcg tggcggatgg taaatttttt ggcaccgcca ggaaaatcaa
     4921 tgaaaccaga gcagggatcg aaggagacgg cagagccgtc ctcgctgaca gtaaccagtg
     4981 cggctttttc cggatacccc ggcgtatcca cacgaaggac atttacaata gcaccggaag
     5041 gatcgacaac ggcattgcct tcaagccacc ctccgaaagt gccgtcaagc cacagcgggt
     5101 cacggggcag cggattgctg aaagtccagc tggaggcatt gaggagatcg gcgtctaccg
     5161 gcgcggacat cataagcgcc tgataccgtt ctccccagcg gatcccgccc atggcgtcct
     5221 caaaggcgcg ccacaatcgg cctttatatt cgatcacggg ccccggtgcg gtgtggtact
     5281 gttcttcgga aggagtgagc cttccgctat gtgcatcgag cggttctgtc cagtgagctc
     5341 cttcgtcatc ggaacgtcgg ataacaattg cgccgtgatg tttttccacg gcaagcagat
     5401 aaacagcgcc gcgatgggta aacagttttg gccagaaaag gcactgcatt tccgcgagcc
     5461 gtgtccagct ttcgccgcgg tcttccgaac ggaatacaac ggctgtcgca catacgtgct
     5521 cattgctttc aggaccgaaa taatcatgcg cggcaagata gtggccggac ggcaggacgg
     5581 taatgctggg cgagcctata tatttcccgg tagactgagg gctgtggctg atgacaattc
     5641 cggggggagc tgcgacgtct gcgccgcggg cagccggaca ggcgacgaag catgcaagcg
     5701 tcagaaggga gagcgtgcca cggaggtaag cagtaacatg catggaaaag cctttacttg
     5761 tggttcagct ggctctgaat ttcatcgctt gctaatttgg tcttgtcctg aatgtgcgca
     5821 agcttcattt gtgccgcacg ggcggaagcg gagcggggct ccactgctat cgcctgctgc
     5881 caggacttta cagcgtccgt atcttcatga agtttgtata aagcattgcc ataataaaga
     5941 agtccccggg aatagtgcgg atcttcctga agcgctttct gaaaagaata cagggcatca
     6001 ctgtagcgct ttgcattgta atgtttcaca ccctgctgca gatacttgcg tgctttcttc
     6061 cgttcacccg tcacagcgga ggaggagcct tcctgccagg aagcggagat gctgtcccag
     6121 aaatcgtggg taaattcggc ggtggaggat cgaaagctga caaagcgcca ggcggtgaga
     6181 gcgaggtcgc ttatgagcgc ccaggcgccg gagaaaaatg acttgctcat aaaggtttcc
     6241 ggtgtgtgcc tttcttgcga ctataaagtg tgatcacttc cttttagtat gcctttttac
     6301 atcttcagtg tcaagtggat cggaaacccg gattagaaag agttgcaaag ccctgtgcca
     6361 ctgcacataa taaaggtaat gaaaaaagat gcaggaaaaa tcatgacaac cccgaatggt
     6421 acaacgcctc tgaatggcag aatagccttt ctggactttc ttttctgctg gccacccaaa
     6481 ggcggcgccg acgtcgatgt gtattccgtg atgacaggat tgcaccggag aggttgtcag
     6541 gtgaagcttt ttgtgcctgc ttttgccgga tgtcccgggc ggggagaagt ggaagcagat
     6601 gtgctttcct ttccggttga aattgttcct gtaccccgaa agcgtcagca actgcctgca
     6661 ctggcggagc agctccaaaa ggcgctcgat ggctggcagc ccgacatcgt gattgtctgc
     6721 cacggctact tgctgaaaac agcccttatt ccgatgctga aggcaaaaac gcttatcagc
     6781 cgcatttatg ccaatgagat cttttgtttg aaagacgctt tccattataa agacggagca
     6841 ccctgtccct tatatctgca taaagaaccg gaactatgca gaaaatgcgc tgctgagcac
     6901 ttggaaacgc aggtaaaaaa gggactgtgg gacccctgga cccatgaata tattattacc
     6961 cggggctggg attcctcttt cggaacggcg catcgtaatg ccctggatcg actggacagt
     7021 atcattgtct ataatgaaag tatcaagggg gcattgtctc cgtgggacag caaggtcact
     7081 gtgattcccg gaggaattac ggctgaaccg gtcgtatccg cctctgcgca ccctgaaaaa
     7141 cagggacagg gtatgcgtgt tttaatggcg ggaagagttg aggaccccct gaaggggctg
     7201 gctgtcctgg aggaagcctg ctgtgccttg cgacggaaag gatatgtttt tgaggtacag
     7261 gtgacccatt atgacccctt tttcggaagg ccggaactga aggcaacagg ctggctgtct
     7321 catgaagaaa cccttgccct gtacaggaat gcccatattg ccgtagttcc ttccttatgg
     7381 gaagaggcct tcggtctggt tgcggtggag gcaatggcgg caggggtgcc cgtatgtgca
     7441 tcacgaaccg gcgggctgca ggaaataata gaggaaggaa agacaggttt tctttttaag
     7501 cccggagaca gtgcagcgct tgcggatgtt ctggaaaaac tactgcagga tccggagttg
     7561 cgtaaaagga tgggcgatgc cgcgcagcag cgggctctgg aggaatatgc ctgggataag
     7621 gtaatcgaaa agcattacat acctttgctg aagggatttc tcccatgaaa aagcgatttg
     7681 cggttctata cagtctggct cctcactttc gacgctcctt gctggcactg cgggaagaag
     7741 agcccgacag ccatataacg cttctgctcc cacccggatt tccgcaggag gttgtggaag
     7801 gatgctgcga tgaaatcctt gcattggcgc ccgcttcgtc gcagcggcgc tctttgaagc
     7861 atgcctggcg cctgatccgg tatcttcgcc gggaacgttt cgactgcttt attgtattct
     7921 ttaacagtcc tgctttgtgg ctgcttaccc aatgcagcgg cgcgcgggaa tgtgcatatt
     7981 tttcgcagcg gggagcccgc tgccccatga gcatgtcttt tcttcgtagt gtatgcgccc
     8041 tggccgggaa aaggatcaga ggacgctata tgtattacaa gattaaatgg gatatctaca
     8101 ggcatccttt ttcggatact tcccgggcag cagacagaaa agaacaggac ggggattctt
     8161 aacccttcag gaaagcaatg ctatggcagg aaaacaggta gagcaggcat tagcactgct
     8221 ggaagggctt aatctgccgt gcttctgtcg cgatgggctt caaaaggcag aagagcatat
     8281 tcgcctcatc cgcgaatgga accatgccat cagcctcgta tcacagggag acctcgcttt
     8341 tttaccggaa cggcacctgg tggacgcact gagccttctg gccgttatca tgcagacgtc
     8401 cggggaaaag cggctgcttg atatcggcag tggcgcaggg taccctgcta tcccgatgaa
     8461 atgtttctgt ccgacgctgg atgtaacgct agtggaacgg agccgccgga aggctggttt
     8521 tcttcagcgt gtcatttcgc tgcttgggct ggaaggggtg acagtgctta ccggagaatt
     8581 tccggcgcag cctgagcgcc tggatgcgca gtggataacg gcgcgtgctg tagagcggcc
     8641 gaaaaaggtt tatgccgatg ttttgcagag acttccgcag gggagtgtgt ttttatgtca
     8701 attggagggg gttccgcctg tagctagtga aatgttccac gtggaacatg tagaggatgt
     8761 gtggcagagc agcgggctca gacggggaag tttgcggctg atccggaaaa aataagacag
     8821 cggggcggat cgggcaaatg ttccacgtgg aacatagtct ccttacttgg gttcattcct
     8881 ggaggtatag cggcggaaca tgcggggaac gcctccatcc cgtgtcacat cggcaaaact
     8941 tgcaggaagg gtggtgcggg tcttgccgtc catcacctga aaatgaaggt gtggaaccag
     9001 actatgcccg acgtgtccgc ttaaagcgat ggcctgcccc tgcttcactt tatccccgac
     9061 cgctactttg ctgccttgct gctgaatatg cccgtaatag gcccaggtgc cgtcgctgtg
     9121 ctcaatggcg accagattgt taggcttttt atagccatga tccgtatgtt cctgcaccac
     9181 tttaacgacc tttccttccc gggcggcacg cacttccgtc ccttcgggca tataaaaatc
     9241 ataggcgtac cgactgctgc catgatggct tacaatgccg cccacaccct gaatacacca
     9301 gtaacttttt ccggcctccc agggcagaca gtagggagag ttttccggat ccggaaaaac
     9361 gacgtcgtct tcccggtttg cccaataaat ggcgaatata accagaggaa tcacaataaa
     9421 gaccaggcaa ccccccgctt taatgatcgg ttttaagcac atgacctttt cctgatgtta
     9481 gtaatgtttt gtttgtaaag cagtgtactg cttcattggt acacaggcgc cgcgcgcaca
     9541 actggtgcgt gttttgcctt aaaaatacct tcccggcact gccgggtata tggtacactg
     9601 gtacaatata cgcaggtact gaaaagtggc gctaaaccaa ttatagcact ctgccggtgc
     9661 cggctgtcga ataaggagtt gtttttctat ggcaaaacac gacttaaacc gccgtgaatg
     9721 gctgcagcgc agcacagcgg cagtgctggg cggtgcatat gccctgcagg cacagagtgc
     9781 agcaccgctt cgccccaaca ttcttttgct aatggcggat caaatgcgcg gcgactgtct
     9841 cggtacagcc ggtcatccgg cggtgcttac gccggcgctt gaccgactcg ctgcagaggg
     9901 cgttcttttc cgccgcgcct attcctcggt tccttcctgc accccggcac gggcggcttt
     9961 gcttacgggg ctcggtccat ggaaaaacgg gatgttgggc tacagtcgta tcccggagca
    10021 gtatgcccgc gaaaaaccgc gcatgcttcg ggaaggcggc tacagtacct gctcgatcgg
    10081 caaatgccat tatcatccgc agcgcaacgg tcacggctat gagaaactgc tcctggatga
    10141 atcaggccgg gtggaagcac cggactttga aagcgactac agacggtggt tccgcatgac
    10201 ggcgcctgat ctgaacccgg atgcgacagg tctgggatgg aatgattacc cagcccgtcc
    10261 ctacgctctt cctgaagaac tgcaccctac ctgctggacg ggtaatgaag cggttcgtta
    10321 tctggagaat taccgggaag atgcgccgtt ttttctgaaa gtgtcttttg cccggcccca
    10381 cagcccctat gatccgccgc agcgtttttt cgacctttat gcggatcgtg aaatccccgc
    10441 tcctgtcata ggcgactggt gcgcccgcta tgcgccccgg gcaagcgaaa aagacagcct
    10501 ctggcatggg gatctgggca ctgatacggt gcgccattcc cgccgtggct actacggcag
    10561 catcactttc attgatgagc agatcgcccg tattctttca gccctggaaa agagcgggcg
    10621 tatggaaaat acgctcattc tgtttaccgc ggatcacggc gacatgctcg gagatcacca
    10681 cctctggcgc aagacctacg ggtatgaagg ttcggcgcat attcccatga ttcttcgggc
    10741 acctgcctct tttgatgtac cctgcaaagg ggttgtccgc gatgaagtgg ttgagctgag
    10801 agacatactg cctacttttc tggatgctgc ggggctgggc ggtgccgaag agatggacgg
    10861 ccgcagcctg ctccagctgc ttcgtgatga tggaagaagc tggcggccgt ggcttgatct
    10921 ggagcattcc cgttgttacg tagattccga acaatggaat ggtctaacgg atggaaaaag
    10981 caaatatctg tttttcggtc agagcggtga agagcagctt tttgatctga cagcggatcc
    11041 gggggaatgc gtgaatctgg cgggcacccc gggtgccgct tccctgctgg aactgtggcg
    11101 ccgccgtctg gctgatcatc tgcaggagcg tggcgctgac tgggtgccgg acggcacatt
    11161 aaagcggcgc ccgaaagatg tactgcattc cccgaactac cggcagcctg aggggtgagg
    11221 gattttctgc ggtcgacggt tgccagcctg ctcatcgttt ttgcctcgtg cacttcgctt
    11281 cgcatacaat gaaccgagcg aatcgccgct agcgataaag gaaaacgcta tgctttacaa
    11341 cacactgggc ggaagtgact tgcaggtctc cgctctttca ctgggtgtct ggcagctggg
    11401 tgacccgggt ttttggggcg aggaggcatg ttcttcgcct gcggatctgg tcgccgccgc
    11461 catcgatcac ggcattactc tttttgatac ggcggaactg tacggcaacg gttcttcgga
    11521 ggagcttctg ggcgcggcat taaaaggcag gcgtgatgag gtgcttctgg cgtccaaagt
    11581 ttccacacag cattgtacgg ctgaaggcat ccgttccgcc tgtgaaaaca gcctttcccg
    11641 gctgcagaca gaccgcatcg acctctatca gatccattgg cccttccctc ccgccgatta
    11701 tgcggcggta caggagaccc tggaagcgct tcggcaggag ggaaagattc gggaaatcgg
    11761 cgtgtccaac ttcggcaggc gtgatctcca gggctggatg gcggaaggga cggctgtttc
    11821 caatcagatc ggttacaaca tgcttttccg tgcacctgaa tatgaaatga ttccagcgtg
    11881 ccgtaagcac aatctcggtg tgctggctta catgccgctg atgcaggggc ttctgacggg
    11941 cagattcaag tctacggaag aaatcccctt tcagcgccgc cgcacccgcc atttctcctg
    12001 cacccgtgaa gggacgcggc acggtcaggt gggacatgag cggcttctgt gggacacact
    12061 ggaggatctc agtgactttg ccgatgccat cggcgtgccc ctttccgtac tgagtctctg
    12121 ctggcttatg cgccagccgg gtgtgtcttc tgtcattatc ggatcacgga aggtggatca
    12181 gctgctgttc aacctgcagg cggcacagct ggacataggt cccgccgcaa ctgcgcagct
    12241 caatgaagcg acgtatccgc tgaaagtggc attgggtcgc aattgcgaca tgtgggagag
    12301 tgacgataac aggcgtatta actgattcgg tgcttgatcc ccgaagctcc ggaaatcttc
    12361 gcctgtcgca tgtgaatgac gaaaggaagg aagcaaacga tcatgaggat cacctttgta
    12421 atccctgcct acaatgaagc gcctacactg gcggctctgg cggagggtat caaagcgcat
    12481 gtgggtgctc atgacttccg gattcttttt gttgatgacg gcagcaccga caattcctgg
    12541 gaaatcctgg aggggctgcg tcgggaagat gcccgaattg atcttctccg gcttcggcgc
    12601 aactgcggaa aaacggcggc gctggaagcg gcatttgcgc tcgtgagcgg ggatgtgctg
    12661 atcaccatgg atgccgatct gcaggatgat cctgccgaaa ttccgcgcat gcttgccaaa
    12721 ctggatgaag ggtatgatct tgtttgcggg tggaagaaaa aacgtcatga tccctggttg
    12781 aaaacaatcc cttcccgttt ttataacgca cgcatttccc ggcgctttaa gttgccgctg
    12841 catgatatca atacgggttt caaagccatg cgcatggaag tggcgaagcg cctgcggctc
    12901 tggagcgaca tgcacaggct tatcccggtt tttgccgcgc atctgggcta taaggtcaca
    12961 gaaattcctg tacgccatca tccgcgtaaa ttcggaaaat ccaaatatgg ttttaccaga
    13021 attcaaaaag ggctggtaga tattgagagt gcagcgtaca ttctgaaata tcaggatgcg
    13081 ccgatgcgca ggatttacat ctctcagagg gggcttcttt atgtgttact gctggttctc
    13141 ctgactctga gtttggcgtt gatcgtgcaa gaggttccgg aggcgcttat actgctgctc
    13201 ttttcaagtt tagctattgc tttatatttt cttaccggtt gctttttcag acttgcccaa
    13261 atgcaccagc tgattctgta ccggatgccg ccgccggatc ccgggcgttt cgtggcagcg
    13321 tatcatgcga ctccctgggc agacgatgcg gagagtcccg gtcaggaaaa ttaaaacaat
    13381 acttttcgaa aacaaggaaa acatgatatg acaaagaaag cggacaacca tcctttgctc
    13441 gggaaaaaag caccggcatt cactttggaa tcaagcgacc gtaaaaaggt acagctcagc
    13501 aattttaaag aggggcttgt tgtcctttat ttttatccga aaaacaacac cccgggctgc
    13561 acgctggagg cgcaggagtt ccgcgatctt cacgatgcgt tcacaaaact gaatgtgaca
    13621 gtgctcggcg tgagtccgga cagcccttct tcggactgcc gtttttccga aaaactgagc
    13681 ctcaattttc tgctgctttc ggaccgggat catgccattg ccgaaaaata cggggtctgg
    13741 gtggaaaaaa gcatgtgcgg caaaaagtac atgggtgtgg agcgcaccac ctttttgatt
    13801 gacgggagcg ggaagatcat tcatgtgtgg ggcaaagtga cggcaaaagg ccatgcgctg
    13861 gcggtactgg atcttattaa aaatcgtgca taagaaggca gtgaaggagc gcattcaggc
    13921 gcttgaatct tgacgcggaa aaagggtatg ccatggttgt ctcgacgctg gactgggtga
    13981 ttgtcgggtt tttctttctc ttttatctgg gtgtcgggct tatattgacc cgccgtgcag
    14041 gatccagtac agctgaattt tttgttgcag gaaggagtat gccctggtgg cttctgggtg
    14101 tgtccatggt ggcgaccact ttctccaccg acacgccgaa tctcgtggcg aatattgtcc
    14161 gagaagacgg tgtcatgggc aactgggtct ggtgggcctt tttgcccacg ggcatgctga
    14221 ctgtttttgt ttatgcccgg ctctggcggc gttccggact gcttacggat cttgaatttt
    14281 acgaattgcg ctacagcggc aagtctgctg cctttctccg cggctttcgc gccatctatc
    14341 tgggtgtctt tttcaatgtc attgtcatgg catctgtttc gcttgccgca attaaaatcg
    14401 gcagcgtgct gctgaacctc acgcccatgc agacgatttt aattgcaagc accatcaccg
    14461 tgatctacag ttcctccgga ggattgcgcg gcgtcctgtt taccgacctg attcagttca
    14521 ctgtcgccat gatcggtgcc gttgccgccg ctgtgattgc tttgcgccac cccgatgtgg
    14581 gcggcctttc aaagcttctt gcccatccgg cgttggaggg taagctctcc atcttcccga
    14641 gctttacgga tccggatcag cggggagtgc ttatctccgt ctttctcatg cccattattg
    14701 tccagtggtg gagcgtctgg tatcccggtt cggaaccggg cggcggcagt tatattgcgc
    14761 agcgcatgct tgccgccaaa gacgagaaaa acgctgtcgg agccgtcttt cttttcaatg
    14821 ccgcccatta tgcgctgcgt ccctggccct ggattgtggt tgccctctgc tcccttctgg
    14881 tatttcccag cctggactcg ctgcgcgagg cttttcccca tgccgcagcc atcatcggtc
    14941 atgatatggg ctattcggcg atgcttacct ttcttcctgc cggtgttctg ggtctggtaa
    15001 tagcctcgct gattgcagcc tatatgtcca cgatttccac gcatctgaat tggggtgcat
    15061 cctatgcggt tcatgacttt tacagccgtt tcctgcgccc ggacgcttcc gaaaaagagc
    15121 tggttctggt tggccgtgta gtgaccgtcg tgctgatgat tctggcgggt ggtgttgccc
    15181 tgatcatgga atctgcccgc gataatttcc agattatgct gatggtgggt gccggcaccg
    15241 ggcttatttt tatcctccgc tggttctggt ggcggatcaa tgccgcaagc gaaatcgccg
    15301 ctatgattat ttcttttttc gttgctcttt attttcggtt tctgcaccat cgcttcggtt
    15361 ttctcccgct cagcgatgcg acccagctgc tcctgactgt ggggattacg acggcaggct
    15421 ggcttgctgt tacctggctc acccgcccgg tggacgagga agtgctgcag cggttctgca
    15481 gcctgatccg ccccggcggt cccgggtgga aagggattcc gattgatccg caatccgtcc
    15541 ctcacggaga tacggcatgg caggtgcccc ggggtattct atgcatgctt ctgggatgcc
    15601 tctccatcta cagtgccctt tttgctacgg gttatcttat ttacggaaac tatctgcccg
    15661 gcgtgctgct gtcggcaaca gccgtgatct ccggatacgt tctggttcgc cagtggcgtg
    15721 ttctcctggc ccgtgactga taagcccgcc gtcagggctg ctgccagccc tgtgcaccca
    15781 ggagcggaac gaagcggcag ggcgtgcctt cttcccggtg aaatccgttc cggtgccgcc
    15841 tgatccgaat caggcgctgg tgctggcggt cgcccaccgg tgctaccagc atgccgcctt
    15901 ccttcagctg tgccaggagt gcttcaggaa tctccggtgc gccggcagtg accagaatgc
    15961 cgtcgtagag cgccccgctt tcatgacctg tactgccgtc tcccaaaaga ggtgttattc
    16021 cccggtatcc gagttctgtc agccgttttg aagcgttatg ataaaggacg tcaatgcgtt
    16081 ccacagtgaa taccgtgtgg gcgagaagcg acagcacagc agcctgatac ccggatccgg
    16141 tgccgatttc aagaagccgg tgttcaggtg acgggcagag ccattgggtc atgagtgccg
    16201 ccatataggg ctgggagatg gtttgctctg cgccaatggg cagcggtctg tcctcatagg
    16261 cctgcgtttt ccagtccgca tcaacgaaga gatggcgcgg tacggacaga aacgcagcca
    16321 gtaccccggg gtctgtaatc ccccgtttgc ggagctgttc ctcaaccatt attttacgca
    16381 gggagtcata atcgggttca tgcaatgtgc tctatttcct ttcttattct attgtgtcac
    16441 agtgtgcgcc cggctgcaag tacgggattt tatagatttg cgatgggcag attttgcgct
    16501 tcctcctgtt ttttagtatc ataatgacct taaagggcac ccgtattttt atatacgcca
    16561 gttttcgtac tttaaaaagg ggcgggtgct gtccagtcag ggctgaaact gattagggct
    16621 ggagggtcgg tactgctttt aaaaaggcag atccgtcaca ctttcccgat tgaggatcgg
    16681 aatccgcttt cagtcttacc aaaataccag tctgtatcag gccggcagaa ggagttctgt
    16741 atttatgaaa aattgcgatc aggtgcttac caccaacagt attgtgacgc attggattga
    16801 ggaaatggca gctttatgcc tgccggacaa gattgtctgg atagacggct cggacgagca
    16861 gcgccaggaa ctgattgcgg aagcctgccg taccggcgag attattgccc tcaaccagga
    16921 gcagtacccc aattcttatt atcaccgcac acatccgctg gatgttgccc gtgtggagga
    16981 caagaccttc atctgctgcc agcgtgaacg ggatgccggt gcgactaata actggaaaaa
    17041 tccggaagag gcgtatacgg aagccgccaa ttttttccgt ggtggtatgc gcgggcgggt
    17101 catgtacgtc gttccctttt ccatgggacc tgtaggctct cccttcagca agatcggtat
    17161 tgagctgacc gacagcattt atgtcgtatt gaacatgatg ataatgtgcc gtgtaggcaa
    17221 acaggttctg gatgtgctgg ggcacagcga tgacttcacg cgctgcctgc acagcaaggc
    17281 gacactggat cctgaaaaac gtcgcatcct tcacttcccc gaagataaca ccatctggtc
    17341 ggttaattca ggctatggcg gcaatgtgct tctgggtaaa aagtgtcttg ccctgcgcat
    17401 ggcgagttgg atggccagaa aagaaggctg gctggctgag cacatgctga ttctgggtgt
    17461 ggaagaaccg agcggtcgtg ttgactatgt ggcggctgcc ttccccagcg cctgtggcaa
    17521 aaccaatctt gccatgctga ttccgcctga agggctgaag agcaaggggt accggatctg
    17581 gactgttggt gatgatattg cctggattcg cccggacacg gacggtcgcc tctgggcgat
    17641 taatcctgag gctggattct tcggtgttgc cccgggcacc aattcctcgt ccaacagaaa
    17701 tgccctgctg accgtccgga aaaacacgat ttttaccaat gtggtgctgg gtgaagacgg
    17761 taccgtatgg tgggaggacg gagaaggaga tcccccggcg aatgcgacag actggctggg
    17821 caacccctgg tatccgggaa agaaagatga aaacggcaag gaaattctgg gtgctcatcc
    17881 caacagccgc tttacggcac cgctgaacca gtgtccttcc gcttcccacc gcatggaaca
    17941 ccatcacggt gtgcctattt ccgcctttat tttcggcgga cgacgcaacc atctcgcccc
    18001 gctggtgtac gaagcatatt cctggaacca tggtgttttt gtcggtgcca gtatggcatc
    18061 ggagcgcact tcggcacagt acggcaaaca gggtgaagta cgtcgtgatc cctttgccat
    18121 gctccccttc tgtggttaca acgttgccga ttattttaag cattggattt ccatcggccg
    18181 ttccatcaaa cacccgccga agattttcca tgtcaactgg ttccgtcgcg acgaaaacaa
    18241 taaattcctg tggcccggct acggcgaaaa tctgcgtgtg ctggagtgga ttcttcagcg
    18301 ggcacgaggt gaaggggatg cggttgaaac gccgatcggt tatattcccg gaaaagagtc
    18361 gctggatatg accggtcttg agcttgctcc ggagacgctg gacgaacttc tgacggtgaa
    18421 ccgggaagac tggaaggctg aggcggacga catagaacag tttttcgaca gcctcggact
    18481 gaaagtgccc tgggaattgc gaaatgagct ggaagccctg cgcaaccgcc tgtcggatga
    18541 cgaataaaca gcgcttttac tgattctgca gataacagct gcgcccggtt tcaaatcggg
    18601 cgcagctttt tagttgttat gccctgcagg gctgtgccgt ggcatctcgc ctttgaaatc
    18661 gcccaggctc tcccaataaa gccgccggct caagtccact tccgccacag cgatactgcc
    18721 ccattccata gcctgcgcca gaacacgccc ctcctgatca taaatgccgg ttatcatcca
    18781 gtgcatcgat atgtctgtat agctgctggc tgccagatac acatgatttt cacatgcccg
    18841 tgcagccgca agcaggggat tgcatcccgc cacgggaaaa gcaatgattt cggcgccatt
    18901 cagggcaagc tgtcgtgcag gctccgggaa aaatccatca tagcagatca tgattccaat
    18961 acggccgata ggggtgctaa aaacaggata gtctgttcct ggcgtgatcc ctgcctcgat
    19021 ctcattgcgg ggcagggacg ctttccggta ttttccgacc agggcgcctg ccgggtccgc
    19081 gagaatggcg gtgttataaa taagggagcc tgctctttcg gtgagccccg cgacaatata
    19141 taaatggtat ttttctgcca tctcggcgaa atagtcactc atcgttccgg ggattgtttc
    19201 tgccatttcg tgatgcggtt tgcctgttcc tgtcgtcgga atggactcgg ggagtaccag
    19261 cagatccact tttgcttcag aagcctggcg aatcagcggc tcaaactggc ggcagctgtc
    19321 cagaccgctt gccgcattac ggggcacata atgcacggca cccagacgga cccgccgggg
    19381 tggcggtgct tccacggggg tgagccggat gtcccgccat tctacagatg ccttccttgc
    19441 ccagcgcaga tacagttcta cctgcacggc agcagcaccg tccggtgact gataggtgcc
    19501 tgagacgtgc gaccagccgt cttcatcggg cccaaaacaa acagggtact caggttcaga
    19561 ccggggattc cggccgccct gataactgtg tgcacccggt tcaccatggt aaagcgcttt
    19621 gccgtctccg ctctgccaga gcaggcgcgc cagaacagag cggcgcggat attcaacgcc
    19681 ggatgcctga taccgggcat cgaagcggta ccactgccct cctgttacgg gaaaggattt
    19741 aaaccagcat ccgtcaagcc cttccctgtc atcggcagag atgcgcagcc ttccgtcggg
    19801 aagcctttca aaggaggggg caatttcttt tctcggggcc cggctttccc agtccttcag
    19861 ctcggtggca ctgcatacgg cagcgatgat cataaaaaaa atcagccgca acggcatgcc
    19921 gatggaagtg ttcatgggaa aggagccttc cttttgcctg cttcagagat atcccagtgc
    19981 ctccagtgag cgcacatctt cgagattgat gctgtaatcg gattcgtcgc cgatgccgaa
    20041 atgctgacga agagcggcat cctcttcttc ggcttttttg agcaacgcct gcgcttcagc
    20101 gacaagttcc ggcacatccg tggcaatgga gtgcttttct gcggggtcta tgctgagatc
    20161 aaaaagctct tcagcggaag aagtcaggga gcggatgtat ttcttattac ggaaaacgac
    20221 gctttccaga gggtcataaa aaatgtttgt gccgataaat acgggcggtg cgctctctcc
    20281 tggctgctca ttatgcaaag agacagggaa agtgttgggc gcaagataag acggaacagc
    20341 ctgtccgcag ctttccatca gggtgggcat tatgcccaga gtggtcacgg gtacctcgac
    20401 aatgcctgtt tttatactct ttggctgctt cagcagaagc ggaacctgta ccagcgggtt
    20461 gtacaggttg tgcccgtgct cgaaatggcc gtgctcccag aattcttctc cgtggtcggc
    20521 tgtcagaaaa atgagggcgt cgtcatagag tt
//
LOCUS       DAOPWB010000052        20013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2715_length_20013_cov_5.460667, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000052 DAOPWB010000000
VERSION     DAOPWB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 20013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20013
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2715_length_20013_cov_5.460667"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..1363
                     /locus_tag="PKN92_06580"
     CDS             341..1363
                     /locus_tag="PKN92_06580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNZ48496.1"
                     /translation="MKKWTVVLEPHEQEDASVIKLREIHFWTLCAVVCLLAFLLAFFF
                     QREREFSSRTELLSRANQRLQQENQDRAAAMPAQIPDNTAGRDAVRQAEARLRSEYEA
                     SIATITAELTDLYDMEKRARSITGLQPRKAARLDSSAMTLNGKGGGPSSTGPFEYVGE
                     KYTVSLPNLIYGSSRPSADLIIEEIRLRTRSLGELVRDMEVQKERIERVPAGWPLARG
                     VGRITSNFGYRRDPFSRRVRHHDGIDISARHGTAVRATARGKVVKAEYMSYYGNMVII
                     EHGDGLSTGYAHLSAMNVRPGATVGRGDVIGAVGSTGKSTGAHLHYEVRIQGRQVNPA
                     KYLVDR"
     gene            1360..1776
                     /locus_tag="PKN92_06585"
     CDS             1360..1776
                     /locus_tag="PKN92_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNZ48497.1"
                     /translation="MMQDPRTNKGTDADMTTLGSGARLKGDLHCSGSVSIEGMVDGTV
                     RSQGTVLLQESARVKGELSGARIVISGEIRGNVQAGERLEIQASGKLLGNITAPVIFI
                     QEGAFFEGQCSMQAEKDKAAPGGDKTETAADKSAKS"
     gene            1948..3867
                     /locus_tag="PKN92_06590"
     CDS             1948..3867
                     /locus_tag="PKN92_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HNZ48498.1"
                     /translation="MPQQKPKTKKPWVYLNPPREVITLLENAPSVITASTTEELVQMA
                     CGGPDGDRCEVAYDVPGQGRVVEATVARVRNGICVNYPEPYMRRRDGDCMVLGDSLPT
                     DKETFDARFGKAFDPLRKETLDWLGKQDLAVFGFIAGMDKMGMDAMVVAPANAGFFVL
                     GLAMLQGIIAVEDLSPKFSPRLIIYIAPPFRHTHFDGKQVVVHQRDAGCHEIFAYNLY
                     PGPSAKKGVYGSLLGLGEREGWVTAHCSVVQVVTPYDNTLTLMHEGASGGGKSEMLEV
                     AHREPDGRLLLGRNIETGEEQRMEIPRGCALRPVTDDMALCHPSLQKGDGKLSVVDAE
                     NAWFVRVNHITSYGTEPHFEALTAQPSEPLLFFNIDAVPGSRALIWEHTQDAPHQPCP
                     NPRVIIPRHIVPDIMNEPVSVDVRSLGVRTPPCTAAHPSYGILGLVHLLPPALSWLWR
                     LVAPRGHDNPSIVDSGGGMSSEGVGSYWPFAPGLMVDHANLLLNQIVSTPKVVHILCP
                     NQYVGAWKTGFMPQWIAREYLARRGIAKFKADQIRPARCPLLGHTLHHLTLEGHPVPR
                     WLLQVNAQPEVGDYAYDEGAKMLTEFFHSNLKQFMKPELHPLGKTIIECCLDGGQVSD
                     YQSLIATTYIATSLS"
     gene            3944..5770
                     /locus_tag="PKN92_06595"
     CDS             3944..5770
                     /locus_tag="PKN92_06595"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNZ48499.1"
                     /translation="MITLHPGESLQSAAARSGHDLSMPCGGQGRCGKCRVRFAENAPA
                     PEGREGHFFSEAQLEAGWRLACIPRSAKEANVFLPPEVLVSSPHQIMMAGRGSQAHKT
                     APRVSKAFLSMNTPTADDSESDLLRIERAWHAAHPDRRKETLHAPLPLLQTLGARLRS
                     QGFSGTAVVREGELLDFEEGDTTDALYAAAFDLGTTTLAAALLDMRSGAALASAAEMN
                     PLITWGDDVISRITAAAGNSDTLKAMQAALLKKIARMLEAMAREAGIALQHIYEIAFA
                     GNTTMEHLLCGVDPAPLGQVPFVSAFSRSHRYAARHFGLPVHSEAECHVFPVIGGFVG
                     GDTVAALLATDFDAGDGIRLLMDAGTNGELVLAHGDRLWAASTAAGPAFEGARISRGM
                     RAARGAVEGVLFRENTVSLAVIGGGVPRGICGSGLIDLCGEMLRTGVLDKSGCMCSPE
                     DGAPFPKSRLRYDRQGGVEFVLYSSESGDITVTQQDIRELQLGTAALRAGMTLLLRTA
                     GLSPEDLDVLFIAGGFGSYIRMEHACRIGLIPEGLAPERVCIAGNAALSGVKWASVST
                     EACALADSLARRVMHIDLSTVPDFAETFVLATLFPDTEPDTL"
     gene            5817..7490
                     /locus_tag="PKN92_06600"
     CDS             5817..7490
                     /locus_tag="PKN92_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNZ48500.1"
                     /translation="MPYPKIARQAVNTVRFAEIVQVFIRHGFKDLVQRAGLHKGFPGK
                     LLHGLRLVNAPVGEPATLGERLRNALTDLGPTFIKLGQILSTRPDLVGHRIAGELTAL
                     RDEVSPLSFEQMRPILEEELGGRLESHFASFQETPVASASLSQVYQAQLHSGEKVAVK
                     VQRPEIERTIEADISLLETLAEWVYDYQKGKGVLDYPGIVDEFARSIRRELNFQIEAR
                     VAEQFAANFSDTESVVIPAVFPKYSSRRVLALQWIDGVPVDRVDAYEVHDSDPAVIAH
                     LGCDTVCKMVFEDRLFHADPHPGNIFLLSHNRIAFLDLGMAGHLEDSDVQALLALFSA
                     MFHGDAQQCVEAVQHLTSTTLPENEDALAHEISEFIAFDAPALINRGEVARGFEIAVQ
                     IMRRHNLELAPRFSLLLKALVTIENVGKALDPHMDMLPIIRPHVERMFLARLRPRNIL
                     HEMQAHADGYLQLSRNAPVDLAHLLKQMRRGNMTFNIHHEHLENLANTIDRSSKRNAV
                     AIVIAALVVGSSMLTAAGVSYARLGSIGFIVAGVLGLTLIVSIVWKGNR"
     gene            7514..7729
                     /locus_tag="PKN92_06605"
     CDS             7514..7729
                     /locus_tag="PKN92_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48501.1"
                     /translation="MSTIRTLLPWGLFLVMSLLTAFFWHQSNRRGQIIDEISGEMLQI
                     QDQLQHVYQELLDLKAFAQEKLGTPEL"
     gene            8196..9749
                     /gene="rny"
                     /locus_tag="PKN92_06610"
     CDS             8196..9749
                     /gene="rny"
                     /locus_tag="PKN92_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNZ48502.1"
                     /translation="MIYAISAIAGVIIGALVMVGVSRVRAGSYLSLARREAAQILSDA
                     ERDSAALIKEGKTTLKELEMNLRGKVENEGRELRKEISAIEKRIAAKEQSLDSRAQTL
                     DKAAASLTSQERSLAAREKENEKERERLSGMLAEQMRQLENIAGLTADQARKEIFVRL
                     ENEVKRDCSLELKRVEDELRENAEKKARWIIGEALQRCAADHVAESTVSVVALPNDEM
                     KGRIIGREGRNIRTLESATGISVIIDDTPEAVVLSGFDPVRREVARIALERLIEDGRI
                     HPARIEDMVEKVTQEMTVCIRERGEQACLDAGVHGLHPEIVKLLGRLSFRTSYGQNVL
                     RHSLEVCHLAGVMAAELGVNVQEAKRAGLIHDIGKALTHEVEGSHAVLGHDMAKRYGE
                     NEAVANAIGSHHAEMPITSLVSVLVQAADAMSAARPGARSEMLQHYIKRLEQLEELVN
                     GFSGVERSFAIQAGREVRVVVHPDKVSDAEAAQMSRDLARKVETEMTYPGQIRVTVVR
                     ETRAVEIAK"
     gene            complement(9807..10919)
                     /locus_tag="PKN92_06615"
     CDS             complement(9807..10919)
                     /locus_tag="PKN92_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ48503.1"
                     /translation="MNLLSLFKKAWRTSNVVIARHLRRPPRYMPTLVLFVTNRCNLRC
                     RMCGVRDLPCAFDREQELTTDDWKKVITSAASELGTFLTTISGGEPLLRPDIFELIGF
                     AAQSGISVHLCSNATLLTEDKILRLKEAGLASISISLESPEEMLHNYLQAEDTFSKVV
                     QSLKDLRRLAPNIRVGINFLITKKNYLEMVPMLEFARSLDMHQIKYSPIHANLLHRRK
                     AMAEYDDLFFEKEDLADLSSELEKLRKLCKKSGLRSSSDAFFRGIADYYEKPRQFMCY
                     AGYSVCAIDPTGNVAPCCDKDSHFNVKQKRLAEIWRDPEFYRLRNLVHHCNVPCWDAT
                     YTEMSLMLQPRSLLLNVFNLLKDVKFYFPSGSDSTH"
     gene            11164..13164
                     /locus_tag="PKN92_06620"
     CDS             11164..13164
                     /locus_tag="PKN92_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48504.1"
                     /translation="MRFLYCSFLCALLVSLTACAEEETLMYYRDIYPDTWVATDGLGR
                     TMPDCGEAGPVKKDQRRIVGMFYITWHGDHLHHKKSPYSSDVTRVLEGDPAARFDGKH
                     PLWKDNFYHWGEPEMGYFLSKDEWVIRKDISMLSDAGVDVLIMDVTNAVRYWEEWEVV
                     FSTLEKMKAEGNKVPYFTFWSFNGPAIVVVRDLYDRIYKEEKYKDLWFYWDGKPLLLY
                     NSTPSVDANGSDIKNPNPNYNPAAATDPAHPHYQDPEYTEEFYTDYPQEIRDFFTLRT
                     MWWGYYEWAGKRAVGAEDYWSFGYQLNDEKVRALDPADLLSTHNGKPEQAAVTPAQHS
                     NSMTGKCWTRENFEPSLNEYDMPVPSFVPWLGETVAHPEDYGIYFQQRWDEILPQNPQ
                     FLYINDWNEWTAGKYHPEEGHTVQFLGRENNYFFVDQYNAEFNRTIQPMKGGYTDNYY
                     MQMAQNLRKYKGVRPIPQQQGISRIIVDGDFSDWDPIRIEYRDTIGDTFHRNYKGYGG
                     LVYVNESGRNDIITSKVAVSEESLYFYVETKEEMTVHSDPNWMLLLIDADQDSSTGWY
                     GYDFLINKEILSDKKTTVKSYTGICPDGRWETVAEIPFRYTGKEMELAVPRALLGLGD
                     AAFSFDMKWSDNPAELKDPISLCTDGDTAPNRRFNYRVIWKN"
     gene            13222..16578
                     /locus_tag="PKN92_06625"
     CDS             13222..16578
                     /locus_tag="PKN92_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ48505.1"
                     /translation="MKFVRLILISLLFYTSAAYASAPEVLWNYESPGGHVDASPALGV
                     LKPGGELNIVVGTTAGVIVALTEKGSEVWRSEVDGPACISPSIGDMDGDGAPEVLAMT
                     QAGSLYCLEGATGQKRWQFALPGRINWGETALTLMDLAGDGGLHVVTGDKEGNVVCVN
                     ASGEMRWIFREDMGVVQAPAAADLTGDGVPEILVAGNKVPLICLSARGEELWRIPSGV
                     GASPVISDMDGDGIPEIITGISQELVLLNTRGEILWKYPMRLSLDSAITVTDLDGDGV
                     SEIVAIDLAGELVCLSAEGKKRWEANVVDRVRRSPSVGDLNGDGILEIVVAGYSGALH
                     IFRADGTEMIRYPVSGGMNTTATLLPLSDGGPGVLIAPANSAMTLIRFPGAKADASLP
                     WREYRQNAMRTAFAQKDSVAPAVKLSLDPGARHVGANVFTALVENPEKEKLTLILEGG
                     RIGGAPLSDSITSSDEIFSIWLSYAVPAGHSADYKFTCTVEQAGRQVLKQTREMHTTP
                     FMKELGDSEAILKEVETLFEKVSDIRGLEERACFLAGRLEKFRPRFFAAPALSDADLL
                     DLKNDFTAFYPELQLLLKMTRAAAIAAEKGSAVLTAAADPWAPFGGVDEIFEGRISDN
                     TLSVEAFPGEHESAALNVFNMSADTVTFRVTLSDLTQGETEKTATEYITLHEAVPVPT
                     EMIDYSVDALPRLNNAALLQVPPRGAVQLWLDINTKGLAPGEWNCKVMLASLQCDSIR
                     TEVPLTVRLYDTALPEEQPLSLCHWGYVERSMLRHIPEKALEDQISHGTNVFVGTIFP
                     QAQYNEQGELVSGPDFSAHDAYVKAHAPHGIILFCGYQHSLRGPGGVNSDAYAKAHKE
                     WLKRWVAHLKELGVGYDGFALYPVDEPGLNDGLVEQYIRMATLAREADPQILLYTDPV
                     MRITEAELEAMVPLVDIWCPHRKGLVLEPSSAKKFEIIRNSGKTVWTYECDDNVKHHS
                     PVGYYRCQAWLAKSRSLTGIGFWSYCTSQDDPWFRPGVRHDYLMVYPGTDVVSSKRWE
                     AVRDGVEDYTLLSLLEKRAEKAEGPLKAEISLFLKETVDAIARYCGDDEDGTLPGPGG
                     EPEVRRVTDRRVEHLKAARREIARYLSLLH"
     gene            complement(16654..19158)
                     /locus_tag="PKN92_06630"
     CDS             complement(16654..19158)
                     /locus_tag="PKN92_06630"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNZ48506.1"
                     /translation="MAVMWKWIRRFLAGLLLLTGIAAVLLSVHVYQTVSWSYRCLPDW
                     DGTVAGCPLDNSVTVLRDEWGIPHIRAATEADAHFALGYCMAQDRLFQMECFRRMAHG
                     QLSEILGPPLVKADCLIRLMGVGDLAQRVAAHPEELFPEEMLSSMHAFLAGINHFIGE
                     GVLPWEFHALKIPCRPFALEDCLAVAGTLPISFADGLRTDLMKSMIREKIPEYDADVL
                     FNGLEKIPVTIMESLEEATALQQAWMEEEKPTAETIAGVHQAATWLRLVMSPDWRQAP
                     PGSNSWVLSGDRTKSGKPILANDPHIIFTNPSIWYEAHVSGGSFECYGYYMTPLPVPL
                     IAHNDFCGWALTMLNTDDTDLQLLRLNPDNPNEYFHEGNWHPFTVEQHRISVRFGKDR
                     YYECRSCPLGVLCCDFFKLLHAYDGPPLAVSWSGKTGGCFADLCGFYEMAHARNYEAF
                     EQGVRKITAYRVNVSYADRDGTIAWWGTGTLSYYPPGVDSKSVIPAEKAASVKRNLYH
                     AQDWLHLKNPPEGMIVTANNLPTAGPVGTAPCEISRLSGYFKPGDRAGRIREILESRK
                     DWTIEALRAVQTDIQGYALRPLVSRMIALAESGMKEQDAASEEALRRLKRWDGRQSTD
                     SVETTLFHFWLDSLFQELFSDKLDPELFRLYVACEDHWYAMQQLLDDRENLWWDRRAT
                     EDKETAAEAAARALQDACVRMKKELGANMRDWRWGRVHQLTFTHPFGYLPLLGRRVNI
                     GPLEAPGANQTINNMVSKELHRYPVIAGPSTRRLIDFADPGRSLSILPTGNSGHLRSP
                     HYADQAPLFMRGEYRYAFLSRDEAEKHGMHRLTLQP"
     gene            complement(19321..>20013)
                     /locus_tag="PKN92_06635"
     CDS             complement(19321..>20013)
                     /locus_tag="PKN92_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48507.1"
                     /translation="PYGLFSYYRIFSTNHRYVESMRALPAETELHGRDCLEVRWQAAE
                     GRPYVLRGIYHWVEAGILDVETIVEAVEDLPDFEVFLSSYMTEGFPSAQIFVKKPGGE
                     PSFLKADPAAGAWQAFPGESRGLSLIRDGRWTIPPSPVDWTIPADYAHPLIFRRHEAT
                     GLTVALMARAEDCFAVMAPQDGDAHFSMYFSLFGRTIKAGEKAITTTRLLIGKMDDAE
                     LLRQYGRFCSKS"
BASE COUNT         4511 a         5252 c         5746 g         4504 t
ORIGIN      
        1 ggggtgagca ggaatgacgg gggggggagg agcaggaatg acgagggggg ggaggagcag
       61 ggatgacagc gttaggatta tgcaatgaaa tatagataaa cgcagggagc ggctacggaa
      121 aaaaacaaac aaataattta tttcgcggtc agtcccgcta tttatcccgg gtaagatttt
      181 agctgtcttg atttggggat tgacatattt tgggggtctg tgctatagtg acgtttatta
      241 tggagcgggt tacacagcgg tatgcggcta agtacaggcc gcggacagga caaacctcca
      301 ggcgcataag cgcctcatac accaggagac tgggctgatc atgaaaaaat ggacggtcgt
      361 gttggagccg catgaacaag aagacgcatc tgtgatcaag ttacgagaaa tacatttttg
      421 gacgctatgt gcggtagtat gccttctggc ttttctgctt gcgttctttt ttcagcggga
      481 acgggagttt tcctcccgga cagagttgct cagccgcgcc aatcagcggc ttcagcagga
      541 gaatcaggac cgtgcggcag cgatgccagc gcagattccg gacaatacgg cagggcgcga
      601 tgcggtgcga caggcggaag cccggcttcg ctccgaatat gaagccagta ttgccaccat
      661 taccgccgag ctgaccgacc tttatgacat ggaaaagcgt gcacgcagta tcaccgggct
      721 tcagccaaga aaagcagccc gtctggattc ttcggcgatg acactgaacg gtaaaggcgg
      781 cggcccctcg tccacaggcc cctttgaata tgtgggcgaa aaatatacgg tgagccttcc
      841 gaaccttatt tatgggagca gccgtccctc cgccgatctg attattgagg aaattcggct
      901 gcgtacgcgc agtctcggcg aacttgtccg ggatatggag gtgcagaaag aacggattga
      961 gcgtgtacct gcaggatggc ctcttgcccg cggtgtgggc cggattacct ccaatttcgg
     1021 ctaccggcgc gatcctttca gccgccgtgt ccgccatcat gacggcatag acatttccgc
     1081 ccgacacggc acagcggttc gtgccactgc ccggggcaag gtcgtcaaag cggaatatat
     1141 gagttattac ggcaatatgg tgatcattga gcacggcgac ggtctttcca caggctatgc
     1201 ccatttgtcc gccatgaatg tccgtccggg agccacagta gggcgcggcg acgtgatcgg
     1261 cgccgtaggc agcaccggca agagcacggg tgcgcatctt cattatgaag tgcgtattca
     1321 gggacgtcag gtcaatcctg ccaaatatct ggtggatcga tgatgcagga tccccgcacc
     1381 aacaaaggta cagatgctga tatgactacg ctcggttcag gcgcccggct gaagggagac
     1441 cttcattgca gcggttcggt gagtattgaa gggatggtgg acggcacagt cagaagccag
     1501 ggtactgtgc tgcttcagga aagtgcccgg gtgaaagggg agctttcagg cgcccggatc
     1561 gtcatcagcg gtgaaattcg cggcaatgtt caggcgggtg aacggctgga aattcaggca
     1621 tcgggcaaac tgctcggcaa tattaccgct ccggtcattt tcattcagga aggcgccttt
     1681 tttgaagggc agtgttccat gcaggccgag aaagacaaag cagcgcctgg cggcgataag
     1741 actgaaacag ctgcggacaa gtctgcaaag agctgatcgc ttttccatcc ccctgttctt
     1801 tcccgttgat ggcgccttac ccgtcggtac cgggctgacc atctgctccc gcttgttttg
     1861 tgcaagtgtc tttttcaaat atgctacaat accgcatctg aacagcctct tctctcctta
     1921 ttaaccttat accgaggacc cgccctgatg ccccagcaaa aaccgaaaac gaaaaagccc
     1981 tgggtctacc tgaatccgcc ccgggaagtt ataacgcttc ttgaaaacgc accttcagtg
     2041 attacggctt cgaccacgga agaactggtg cagatggcat gcggcggacc cgacggcgac
     2101 cgctgcgaag ttgcctatga tgtgccggga cagggcagag tggtggaagc gaccgtggca
     2161 cgggtgcgca acgggatttg cgtaaattat cctgagccct atatgcgccg tcgtgacggc
     2221 gactgcatgg tactgggcga cagtctgccc acggataagg aaacctttga tgcccgcttc
     2281 ggcaaagcgt tcgatccgct ccggaaagaa acgcttgact ggctgggcaa acaggatctg
     2341 gcggttttcg gctttattgc gggcatggac aaaatgggca tggacgccat ggttgttgcg
     2401 cctgccaatg cgggattttt cgtcctgggc cttgccatgc ttcaaggcat catcgctgtg
     2461 gaggatttgt cgcccaagtt cagtccccgt ctcattattt atattgcacc gcctttccgg
     2521 catacccatt tcgacgggaa gcaggtagtg gtgcatcagc gtgatgcggg ctgtcacgaa
     2581 atatttgcct ataatctgta ccccggaccc agtgcgaaga aaggcgtgta tgggagtctg
     2641 ctcggtctgg gagagcgcga aggctgggta accgcccatt gttcggtcgt gcaggtggtc
     2701 acgccctacg ataataccct gaccctcatg catgaaggtg caagcggcgg cggcaaaagc
     2761 gaaatgctgg aagtggcgca ccgcgaaccg gacggccgtc tgctgctggg acgcaatatt
     2821 gaaacgggcg aagaacagcg catggagatt ccccggggct gcgcactgcg ccccgtcacg
     2881 gacgatatgg cgctttgtca tccctcgctt cagaaagggg acggcaaact gtcagtggtg
     2941 gatgcagaga atgcctggtt tgtccgggtc aaccacatta cctcctacgg tacggagcct
     3001 cattttgaag cgctcacggc acagccttcg gaaccgctcc ttttcttcaa tattgatgct
     3061 gtcccgggca gccgtgcact gatctgggaa catacgcaag atgcacccca tcagccctgc
     3121 cctaatccgc gggtaattat tccccggcac attgtccccg atattatgaa tgagcctgtg
     3181 tctgttgatg tgcgcagtct gggtgtccgt acgccgccct gcactgccgc ccaccccagt
     3241 tacggtattc tgggtctggt tcacctgctt cctccggcac tgtcctggct gtggcgtctt
     3301 gttgcgcccc ggggtcacga taacccgagc attgtggata gcggcggcgg tatgagcagc
     3361 gaaggggtcg gttcctactg gccttttgcg ccggggctta tggtggatca tgccaacctg
     3421 cttctgaatc agatcgtatc cacgcccaag gtggtgcaca ttctctgccc caaccagtat
     3481 gtgggcgcat ggaagaccgg atttatgcct caatggatcg cccgggagta tctggcacgg
     3541 cggggcattg ccaaattcaa ggcagatcag atccgcccgg cacgttgtcc gctgctgggg
     3601 cataccctgc atcatctgac gctggagggt catccggttc cccgctggct tctgcaggtg
     3661 aatgcccagc ctgaagtggg tgattatgcc tacgacgaag gggcaaaaat gctgacagaa
     3721 tttttccaca gcaatttgaa gcaatttatg aaaccggaat tgcatccgct gggcaagaca
     3781 attattgaat gctgtcttga cggcggtcag gtgagtgatt atcagagcct gatagccacg
     3841 acctatatcg ccaccagcct tagttaggca tgaatgccgg ggataccgga atatgcagta
     3901 cagggcagac tccgcacata atgcgccgcc ggatccggag gttatgataa ccctgcatcc
     3961 cggagagtcg cttcagtccg ccgccgcccg gtcggggcat gacctgagta tgccctgcgg
     4021 cgggcagggc aggtgcggca agtgccgggt tcgcttcgca gaaaatgccc ctgcaccgga
     4081 aggaagagaa ggtcattttt tttctgaagc gcagctggaa gcgggctggc gcctggcctg
     4141 tatcccccgg agcgcaaagg aggcaaacgt ctttctccct ccggaggtcc tggtgtcgtc
     4201 gcctcatcag attatgatgg cagggcgggg cagtcaggcg cacaaaacgg ctccccgtgt
     4261 aagcaaagcc tttctttcca tgaatacgcc gactgctgat gacagcgagt ccgaccttct
     4321 ccgtattgag cgcgcctggc atgccgcaca tccggatcgg cgaaaagaga cgctgcatgc
     4381 gcccctaccg ctgctgcaga cactgggcgc ccgcctccgg agtcagggct tttccggcac
     4441 agcagtcgtg cgggaaggcg aactgctgga ttttgaagaa ggggacacga ccgatgccct
     4501 ttacgccgcc gcttttgatc tgggaacgac cacgcttgca gctgccctgc tggatatgcg
     4561 gagcggtgct gcacttgcct cagcggcgga aatgaacccg ctcatcacct ggggtgacga
     4621 tgtgatcagc cgcatcaccg ccgccgcagg caacagcgat accctgaaag cgatgcaggc
     4681 ggcattgttg aagaaaatcg cccggatgct tgaagccatg gcacgcgaag caggcatagc
     4741 actgcagcat atttatgaga tcgcttttgc ggggaataca accatggagc acctcctttg
     4801 cggggtggat cctgcaccgc ttggccaggt accctttgtc tccgcttttt ccaggtcgca
     4861 ccgatacgct gcccggcatt tcgggctgcc cgttcattct gaagccgaat gtcatgtttt
     4921 ccccgtcatc ggcggctttg tgggcggtga tacggtggcg gcgctgctgg caacagactt
     4981 tgatgcaggg gacggcatcc gcctgcttat ggatgcagga accaatggag aactggtgct
     5041 ggcacacggt gatcgtttat gggcggcgag cacagctgcc gggcccgcat ttgaaggcgc
     5101 acgaatttcc cggggaatgc gtgccgcccg cggagctgtt gaaggggtgc tttttcggga
     5161 aaatacggtg tctctggcgg tgatcggcgg cggcgtcccc cggggcatct gcggcagcgg
     5221 gctcatcgat ctgtgcggtg aaatgctgcg caccggcgtg ctggacaaaa gcggatgtat
     5281 gtgttcaccg gaagacgggg cgccttttcc gaagtcacgg cttcggtatg acaggcaggg
     5341 cggtgtggaa tttgtacttt attcctccga gtccggcgat attacggtaa cgcagcagga
     5401 tatccgggaa ctgcagctgg gaaccgcggc gctgcgagca ggcatgacat tgcttctgcg
     5461 cacggccggg ctttcacccg aagacctcga tgttcttttc attgccggcg gtttcggcag
     5521 ttacatccgg atggaacacg cgtgccgcat tggtctgatt ccggaaggtt tggcgcctga
     5581 acgtgtgtgc atagcgggaa atgccgccct cagtggtgta aaatgggcat cagtgtcgac
     5641 ggaagcatgt gcgcttgcgg attctctggc gcgccgggtc atgcatattg acctgagtac
     5701 cgtccctgat tttgcggaaa catttgtgct ggcaaccctg ttcccggata cggaaccgga
     5761 cacgctgtaa aagcggcgtg cgggtgaata ggccgccgct tttgaaagga agcatcatgc
     5821 cctatccgaa aatagccagg caggcagtca atacagtccg ctttgcggaa atagtgcagg
     5881 tgtttatccg acacggtttt aaggatctgg tgcagcgtgc ggggctgcac aaagggtttc
     5941 ctggaaagct gctgcacggc ttgcgtttag tgaatgcgcc tgtgggcgag cctgcaaccc
     6001 ttggagaacg cctgcgcaat gctttaacgg atctgggccc gacttttatt aaactgggtc
     6061 agattctcag cacacgcccc gatctggtgg gacacagaat tgccggggaa ctgacggcat
     6121 tgcgcgatga agtgtcgcct ttatcctttg agcagatgcg tcccattctg gaggaagaat
     6181 tgggtggcag attggagagc cattttgcct cttttcagga aacgcctgtc gcttccgcat
     6241 cgctgagtca ggtatatcag gcgcagctgc acagcggtga gaaggtggcg gtcaaggtac
     6301 agcgcccgga aattgagcgc acgattgaag cagacatcag cctgctggaa acccttgctg
     6361 aatgggtgta tgactatcag aaaggcaaag gtgtccttga ttatccgggt atcgttgatg
     6421 aatttgcccg cagcatccgc cgtgagctga attttcagat tgaggcccgg gtggcggagc
     6481 aatttgccgc taatttttcc gatacggaat cagtggtgat tcctgccgtt tttcctaaat
     6541 acagcagtcg tagggtgctg gcactgcaat ggatagacgg tgtgccggtg gatcgggtgg
     6601 acgcctatga ggtgcatgac tccgatcccg ccgtgattgc ccatctgggc tgcgatacgg
     6661 tgtgcaaaat ggtttttgaa gacaggctct ttcatgccga cccccatccc ggcaatatct
     6721 ttctgctgag tcacaaccgc attgcttttc tggatctggg aatggcgggg cacctggagg
     6781 acagtgacgt gcaggcgctg ctggctcttt tttccgccat gtttcacggg gatgcgcagc
     6841 aatgcgttga agccgtacag catctgacct cgaccacgct cccggaaaat gaggatgccc
     6901 tcgcccatga aatctcggag tttatcgcct ttgatgcgcc cgcactgatc aaccggggtg
     6961 aggtcgcccg gggttttgag attgccgtgc agatcatgcg ccggcacaac ctggaactgg
     7021 cgccccgttt ctcgctgctg ctgaaagcac ttgtaaccat tgaaaatgtg ggaaaagcac
     7081 tggatcctca catggacatg ctgcccatta tcaggcccca tgtggaaaga atgtttcttg
     7141 cccgcctcag accgcgcaat atcttgcatg aaatgcaggc gcacgccgat ggatatttgc
     7201 agctcagccg taacgcgccg gtggatctcg cccatttact gaaacagatg cggcgaggta
     7261 atatgacttt taatattcac catgagcatc tggaaaatct tgccaatacc attgaccggt
     7321 caagcaagag aaatgccgtt gccattgtga tcgccgcact cgttgtcggt tcgagtatgc
     7381 ttacggctgc aggcgtttct tatgcgcgcc tcggcagcat cggatttatt gttgccggtg
     7441 tcttgggtct gacgctgatt gtgtccattg tctggaaagg aaaccgttga ccatggaatc
     7501 aaaggaacct gctatgagta ccataagaac cctgcttccc tggggtctat ttctagtcat
     7561 gtccctgtta accgcttttt tctggcatca gtccaaccgg cgtggccaga taattgatga
     7621 gatttccgga gaaatgctgc aaattcagga tcaactccag catgtttatc aggaattgct
     7681 tgatttgaaa gcctttgcac aggaaaaatt agggacaccg gagctctgat ctgcatgtaa
     7741 tgagtatact ttctgctgta aatagaacga aagtaaagcg gtgctattgc tgagtttgcc
     7801 cggagcatgc gtagaaagag cagagttatt cttctttgct gaattggaat taatagtaat
     7861 agcacagcgc tgataagaga tgcgatcaca aaagaattga ctgctggcac gcatttcagt
     7921 atactagtac tgttgaacag tcctatctgt gatagcagaa agcatgaaaa caatcgatta
     7981 ccgaagcgat aaaataaaaa caggaaccga cacgatgtgc cggatgccct attttagagc
     8041 agccatattt atcgtctatt tgtggcttct cagcatgcaa ccgccagtat tttcggaagg
     8101 atttccgggg acggcggttt ttttcgtgtt tctctgctgt cttttaattc ccaacttcaa
     8161 gactgatccg gtgacggagc agggagatac tgaacatgat ttacgcaatt tcggcgattg
     8221 ccggtgtaat tattggcgct cttgtgatgg taggggtttc cagggtccgg gcaggcagct
     8281 atttaagcct tgcccggcgt gaggcggcgc agattttatc tgatgcggaa cgggacagtg
     8341 ccgctctcat caaagagggg aagacgacgc ttaaagaact ggagatgaat ctgcggggca
     8401 aggtggaaaa cgagggcagg gaactccgca aagaaatcag cgcaattgaa aagcgcatcg
     8461 ccgccaaaga gcagagcctt gattcccggg cgcagacgct ggataaagcg gccgcctccc
     8521 ttaccagcca ggagcggagt ctggcggcgc gggaaaaaga aaacgaaaaa gagcgtgaac
     8581 gtctttcagg aatgcttgca gagcagatgc gccagctgga aaatattgcc ggacttacgg
     8641 ctgatcaggc gcgcaaagag atttttgtcc gtctcgaaaa tgaagtcaag cgggattgct
     8701 ctctggaact gaagcgggtg gaagacgaac tgcgcgagaa tgccgagaag aaggcgcgct
     8761 ggattatcgg cgaggctttg cagcgctgtg ccgccgatca tgtggcagag agtacggtct
     8821 ccgtggtggc acttcccaac gatgaaatga aggggcgtat tatcggcaga gagggccgaa
     8881 atatccgtac actggaaagt gcgacgggca tcagcgttat tatcgatgac acgccggaag
     8941 cggtggttct gtccggattt gatccggtac gtcgggaggt ggcgcgtatt gcgctggagc
     9001 ggctcattga ggacggccgt attcatcccg cccgcattga ggacatggtc gaaaaagtga
     9061 ctcaggagat gacggtgtgc atccgcgagc ggggcgaaca ggcgtgcctg gatgctggcg
     9121 tacacgggct gcatcctgag attgtgaaac tgctgggacg gctgagtttc cgtacatcct
     9181 acggacagaa tgtgctgcgc cactcgctgg aggtttgtca tctggcgggt gttatggcag
     9241 ctgagctggg ggttaacgtg caggaagcga agcgcgccgg tctcatccat gacatcggca
     9301 aggcgctgac ccatgaagtg gaaggctccc atgcggtgct tggccatgat atggcgaagc
     9361 gttacggcga aaatgaagca gttgccaatg ccatcggttc acaccacgcc gagatgccca
     9421 tcaccagtct ggtttctgtg cttgtgcagg cggccgatgc catgtcggcg gcacgaccgg
     9481 gcgcgcgcag tgaaatgctg cagcattaca ttaagcggtt ggagcagctg gaagaactgg
     9541 tcaacggatt ttccggtgtg gagcgctcct ttgccattca ggccggtcgc gaagtgcgtg
     9601 tggtggtgca tcccgacaag gtcagcgatg cggaagcagc ccagatgtcc cgtgatcttg
     9661 cccggaaagt ggagacggaa atgacttatc ccggacagat ccgtgtgact gtggtgcgcg
     9721 agacccgtgc tgtggaaatc gccaaatagc cctttatccg caccagaagc atgatgtgtc
     9781 ccgccgggtt tctccttcct ggggggtcag tgtgtactgt ctgagccgga aggaaagtaa
     9841 aacttcacat ccttcaggag attgaaaaca ttcagcagca gggagcgggg ctgcagcatc
     9901 aggctcattt cggtataggt cgcatcccag cagggcacgt tgcagtggtg caccagattg
     9961 cgaagccggt aaaattcggg gtcgcgccag atctccgcca gccttttctg ctttacatta
    10021 aaatggctgt ccttgtcaca gcagggtgct acattgccgg tcgggtcgat ggcgcatacg
    10081 gaatagcccg catagcacat gaactgcctg ggtttttcgt agtaatccgc aattcctctg
    10141 aaaaaggcat cggacgaact gcgcaggccg ctttttttgc agagtttgcg cagcttctcc
    10201 aactcggagg acagatcggc aagatcctct ttttcaaaga agagatcatc gtattctgcc
    10261 attgctttgc gccgatggag caggttcgca tggatggggg agtacttgat ctggtgcatg
    10321 tccagcgacc gtgcaaactc cagcatcggc accatttcca gatagttttt cttcgtgatc
    10381 agaaaattga tgcccacacg gatattcggg gcaagacgcc gcaggtcttt tagtgactgc
    10441 accaccttcg aaaaggtgtc ttccgcctga agatagttgt gcagcatttc ttccgggctt
    10501 tccagtgaaa tggaaatgga agccagaccg gcttctttca gccgcaggat tttgtcttcc
    10561 gtaagcagcg tggcgttgct gcacaaatgc acggaaatac cggactgtgc tgcaaaaccg
    10621 atgagctcaa aaatatccgg tcgcagcagc ggctcgcctc cgctgatggt ggtaagaaag
    10681 gtgcccagtt ccgacgcagc ggacgtaatg acctttttcc agtcatcagt cgtcaactcc
    10741 tgctctctgt caaaggcaca gggaagatcc cggacgccgc acatgcggca gcggaggtta
    10801 cagcggttgg tgacaaaaag caccagcgtg ggcatatatc ggggcggccg tcgcaaatgc
    10861 cgggcgatca caacattgga tgtgcgccag gcttttttaa ataaagacaa aaggttcaca
    10921 gagattttct cattttcagg gacagcgttg ccttgaccaa aaaaggcgtt tgtcccggta
    10981 taacttactt cagaatttcc gcaccatcat aaaccacctg gtgctttatg atgataacac
    11041 tggagggggg ctctccattt cacagccggt gccttgcgtt gccgggcatc gcaaagcatg
    11101 atattgctta tggtatcata gacccttatg tgtctgtgta tcatttcaag gtggagaatt
    11161 tcaatgcgtt ttctatactg ctcatttctg tgcgccctgc tcgtttctct tactgcgtgt
    11221 gctgaagagg agacactcat gtattaccgg gatatttatc ccgatacctg ggtggcgact
    11281 gacggactgg gaagaactat gccggactgc ggggaggcgg gacctgtcaa aaaagaccag
    11341 cggcgtatcg tcggtatgtt ttacattacc tggcatggag atcatcttca ccataaaaaa
    11401 agcccctaca gctccgacgt gacccgtgtg ctggaaggag acccggctgc gcgtttcgat
    11461 ggaaagcatc cgctctggaa ggataatttt taccactggg gtgagccgga aatgggctat
    11521 ttcctgagca aagatgaatg ggtgatccgg aaagacatat ccatgttatc cgatgcggga
    11581 gtggatgtgc tcatcatgga tgtaaccaat gcggtgcgct actgggagga atgggaagtt
    11641 gtcttttcca ctctggaaaa gatgaaggca gaagggaaca aggtgcctta cttcactttc
    11701 tggtctttta acggtccggc gattgtagtc gtgcgggatc tctatgaccg tatctataag
    11761 gaagagaaat ataaagatct gtggttctac tgggatggca agccgctcct gctctacaac
    11821 agcaccccct ctgtagatgc taacggcagc gatataaaaa atcccaatcc gaattataac
    11881 cccgccgccg ccactgatcc tgcccatccc cattatcagg atcccgaata tacagaggaa
    11941 ttttataccg attatccgca ggagattcgc gatttcttca ccctgcgtac tatgtggtgg
    12001 ggttattacg aatgggcggg aaagcgtgcg gtaggcgccg aagattactg gagtttcggg
    12061 tatcagctga acgacgaaaa agtacgcgcc ctggatcccg ctgatctgct ctccacgcat
    12121 aacggaaagc ccgagcaggc agcggtgacc ccggcacagc attccaacag catgacaggc
    12181 aagtgctgga ctcgggaaaa tttcgaacca tccctgaatg aatatgacat gcctgtgccg
    12241 tcatttgtgc cctggctcgg tgaaacggtg gcgcacccgg aagactacgg aatttacttt
    12301 cagcaacgct gggatgaaat tctgccacag aacccccaat tcctctatat aaacgactgg
    12361 aacgaatgga cggcaggaaa ataccaccct gaagaagggc atacggttca gtttctcggg
    12421 cgcgaaaata actatttttt cgtggatcag tacaatgctg aattcaatcg caccatccag
    12481 cccatgaaag gcggctacac cgacaactac tatatgcaga tggcgcagaa cctgcgaaaa
    12541 tacaaaggtg tgcgccccat accccaacag cagggcatca gccggataat tgtggacggc
    12601 gatttctccg actgggaccc gatccggatc gaatacagag acaccatcgg tgataccttc
    12661 caccggaact acaaaggata tggcggactt gtctatgtga atgagtcggg cagaaatgac
    12721 attataacct caaaggtggc agtgtcggag gagtcccttt atttttatgt tgaaaccaaa
    12781 gaagagatga cggtacactc agatccgaac tggatgcttc tgctgatcga tgctgatcag
    12841 gacagcagca caggctggta cgggtatgat ttcctcatca ataaagaaat cctgtccgat
    12901 aaaaaaacaa ccgtcaaaag ttatacaggt atctgccccg atggaagatg ggagaccgtt
    12961 gcggaaatcc ccttccgtta tacaggaaaa gaaatggagc tcgcggttcc ccgtgcactt
    13021 ctcgggctgg gagatgcagc tttcagtttt gatatgaaat ggagcgacaa cccggctgaa
    13081 cttaaggatc ccatttctct gtgtaccgat ggagacacag cgcctaaccg tcgctttaat
    13141 taccgtgtaa tctggaaaaa ctagggatat gcgtgcgtta cggtcttttt gtttgtggtc
    13201 aacctttagg aaaatatgct gatgaaattt gttcgactga tactgatttc tttgttgttt
    13261 tatacgtctg ccgcttacgc ttccgcaccg gaagtgctct ggaactatga gtcgcccggc
    13321 ggtcatgtag atgcctctcc cgcactgggt gtgcttaagc cgggcggtga gcttaacatc
    13381 gttgtcggta ccacagcggg tgtgattgtc gcccttactg aaaagggcag cgaagtctgg
    13441 cgcagcgaag tcgacgggcc tgcatgtatt tcgccttcca taggcgatat ggatggtgac
    13501 ggcgcgccgg aagtgcttgc catgactcag gcaggcagcc tctactgtct ggaaggcgcc
    13561 acagggcaga aacgctggca gtttgctctg cccggtcgca ttaactgggg cgaaacggca
    13621 ctgacgttga tggatcttgc cggggacggc ggactccatg tggtgacggg cgacaaggaa
    13681 ggaaatgtgg tctgcgtgaa tgcttccggg gaaatgcggt ggattttccg ggaagatatg
    13741 ggagtggtac aggcccctgc cgccgctgat cttacaggtg acggtgtgcc ggaaattctc
    13801 gtggcaggca acaaagtgcc gctgatctgc ctgtccgcaa ggggggagga actctggcgc
    13861 attccttccg gtgtcggcgc aagccctgtt atcagcgata tggacggcga cggtattcct
    13921 gaaattatca ccggcatttc gcaggaactt gtgcttctga acacacgggg tgaaatcctc
    13981 tggaagtacc cgatgcgcct ctctcttgac agtgcaatta ctgtgacaga tctggatggc
    14041 gacggagtgt ctgaaatcgt tgcaatagat cttgccggcg aactggtatg cctgtcggcg
    14101 gagggcaaaa aacgctggga agccaatgtg gtcgaccggg tgcgccgttc gccctcggta
    14161 ggcgatctta acggtgatgg tattctcgaa attgtcgttg ccggatacag cggcgcgctc
    14221 catatcttcc gcgcagacgg tacggaaatg atccggtatc ccgtttccgg cggtatgaat
    14281 acaacagcga ctctgctgcc tctgtcagac ggcgggccgg gggttcttat tgcccctgca
    14341 aacagtgcca tgacgctgat ccgctttccc ggcgccaaag ctgatgcgtc gctgccctgg
    14401 cgcgaatacc ggcagaatgc gatgcgcact gcatttgcgc aaaaagacag tgttgcgcct
    14461 gcagttaaac tgtccctcga tcccggtgca cggcatgtgg gagccaatgt atttacagca
    14521 ttggtggaaa atccggaaaa agaaaagctc acccttatcc tggaaggagg cagaatcgga
    14581 ggcgcgccac tgagtgacag cattacttca tcagatgaga tattttccat ctggttgtct
    14641 tatgccgttc ccgccgggca ttctgccgat tataagttta cctgcaccgt ggagcaggca
    14701 ggcagacaag ttctgaaaca gaccagagaa atgcatacca caccctttat gaaagaattg
    14761 ggcgattccg aggcgatact gaaagaagtt gagacgctct ttgaaaaggt gagcgatatt
    14821 cgcggtctgg aggagcgcgc ctgttttctt gccggacggc tggagaagtt caggccccgg
    14881 ttctttgcag cacctgctct ctccgacgct gatctgctcg atcttaaaaa tgactttacc
    14941 gccttttacc cggaattgca gctgcttctg aaaatgaccc gtgccgcagc catcgctgcc
    15001 gaaaagggaa gtgctgtgct gacagctgcc gctgacccct gggcaccttt cggcggtgtg
    15061 gatgagattt tcgaagggcg catctccgac aatacccttt ctgtggaagc atttcccgga
    15121 gaacatgaat cagcagcgct taacgtcttc aatatgagtg ccgataccgt gacttttcgt
    15181 gtgacactga gcgacctcac acagggcgaa acagaaaaaa cagccacaga atacattacg
    15241 ctgcatgagg cggtgcccgt gccgacggaa atgatcgatt attccgtgga tgcgctgccc
    15301 agactgaata acgccgctct tttgcaggtg ccgccgcggg gtgctgttca actgtggctt
    15361 gacataaaca ccaaaggact ggcgccgggc gaatggaact gcaaagtgat gcttgcgagc
    15421 cttcagtgcg attcgatcag aacagaagtg ccgctgactg tcaggctcta cgacacagcg
    15481 ctgcccgaag agcagcccct ttctctgtgc cactggggct atgtggagcg gtccatgctt
    15541 cggcatatcc cggaaaaggc gctggaagat cagatctctc acggcaccaa tgtctttgtc
    15601 ggcaccatct ttcctcaggc gcagtataac gaacagggcg aactggtcag cgggcccgac
    15661 ttcagcgctc atgatgccta tgtgaaagcc catgcccccc acggtattat cctgttctgc
    15721 ggttaccagc attcgctgag aggaccgggc ggggtgaatt ccgacgctta tgcgaaagcg
    15781 cacaaagagt ggctgaaacg ctgggtcgcc catctgaagg aactcggcgt gggttacgat
    15841 ggatttgcgc tctatcctgt tgacgagccg gggcttaatg acggtctggt ggaacagtat
    15901 atccggatgg caacactggc acgggaagcc gatccgcaga ttctgctcta taccgaccct
    15961 gtcatgcgta ttactgaagc ggagctggag gcgatggtgc ctctcgtgga tatctggtgt
    16021 cctcaccgca aaggactcgt cctggagccg agcagcgcaa agaaattcga aattatccgc
    16081 aattccggaa aaacagtatg gacttatgaa tgcgacgaca acgtaaagca tcattcgccc
    16141 gtcggttatt atcgctgcca ggcgtggctt gccaaatcac gcagccttac cggcatcggc
    16201 ttctggtctt actgcacctc gcaggatgat ccctggttcc gccccggagt ccgccatgat
    16261 tatctcatgg tttatcccgg cactgatgtt gtgtcaagca agcgctggga agccgtccgt
    16321 gatggcgtcg aagactacac gcttctgtca cttctggaaa aaagagcgga aaaggcggaa
    16381 ggtcctctga aggcggagat cagtctgttt ctgaaagaaa cagtggatgc cattgcccga
    16441 tattgtggtg acgatgaaga tggcacgctg cccggtcccg gtggcgaacc tgaagtccgt
    16501 cgggtaacag acaggcgtgt ggagcatctg aaagcggcgc gccgggaaat tgcccggtat
    16561 ctctcgctgc tgcactaagc cgctgctgat atacggaacc ctcagtttcc tccgttctgc
    16621 ccgccgggac ggaggaaact ttcccttatt ccctcagggc tgcagggtca gcctgtgcat
    16681 cccatgcttt tctgcttcat cgcggctgag gaatgcatac cggtattctc cgcgcatgaa
    16741 aagcggtgcc tgatcggcat aatgcgggct gcgcaggtga ccggaattgc ctgtcggcaa
    16801 aatggacaga gagcgcccgg ggtcggcaaa atcaatgagt cgccgggtgg acggccctgc
    16861 aataaccggg tagcgatgca gttctttgct caccatgttg ttgattgtct gattggcgcc
    16921 cggtgcttcc agcggaccga tgttcacacg gcgcccaagc agaggcaggt agccgaatgg
    16981 atgggtgaag gtcagctgat gcactctgcc ccagcgccag tcccgcatgt tggcacccag
    17041 ttcttttttc atgcgtacgc aggcatcctg cagagctcgg gctgcggctt cggctgccgt
    17101 ctctttgtcc tccgtcgccc gtcggtccca ccagaggttc tcccggtcat ccagcaattg
    17161 ctgcatggcg taccagtgat cctcgcaggc gacatacagg cggaaaagtt ccggatccaa
    17221 tttgtcgctg aacagttcct gaaaaaggct gtccagccag aaatgaaaaa gtgttgtctc
    17281 cacactgtct gtgctctgac ggccgtccca gcgcttcagt cggcgcagcg cttcttctga
    17341 tgcggcgtcc tgttccttca tgccggattc cgccagagcg atcatccgac tcaccagcgg
    17401 acgcagtgcg tagccctgaa tatcggtctg tacagcccgc agcgcttcaa tggtccagtc
    17461 tttccggctt tccagaatct cccggatgcg ccccgccctg tcccccggct tgaagtagcc
    17521 gctcagccgg gaaatctcac agggtgccgt gcctacaggt cctgctgtcg gcagattgtt
    17581 cgccgtgaca atcatgcctt ccggcggatt tttcagatgc agccagtcct gggcgtgata
    17641 aaggtttctt tttacagacg cagctttttc cgccggaata acggatttgg aatccactcc
    17701 gggcggataa taggaaagtg tacctgtgcc ccaccaggca atagtgccgt cccgatcagc
    17761 gtagctgaca ttcacccggt aggcagtgat tttgcgtaca ccttgttcaa acgcctcgta
    17821 gtttcgcgca tgcgccatct cataaaaacc gcacaaatca gcgaaacatc cccctgtctt
    17881 gccgctccag cttacagcaa gcggcggccc gtcataggca tgcagtaatt taaaaaagtc
    17941 acagcaaaga acacccagcg ggcagctgcg gcactcataa tatctgtctt ttccgaaacg
    18001 tacggaaata cggtgctgct ccacggtaaa aggatgccag tttccttcgt gaaaatattc
    18061 attcggattg tccggattga gtcggagcag ttgaaggtct gtgtcatccg tgttgagcat
    18121 ggtcagcgcc catccgcaga aatcattatg cgcaatcagc ggtacgggca gcggggtcat
    18181 gtaatagccg tagcactcaa aggaaccgcc cgacacatgc gcctcatacc agatcgacgg
    18241 gttggtaaaa atgatatgcg gatcattggc aaggataggc ttgccacttt ttgtcctgtc
    18301 acccgacagc acccaggaat tactgccggg cggcgcctgc cgccagtcgg gcgacatcac
    18361 aagtcgcagc caggtcgccg cctgatgcac cccggcgatt gtttctgctg tcggcttttc
    18421 ctcttccatc caggcctgct gcaatgccgt cgcttcttcc aggctctcca ttatcgtgac
    18481 gggaattttt tccagcccgt taaagagtac atccgcatcg tactccggta ttttttcacg
    18541 gatcatgctc ttcatcagat ccgttcgcaa accatcggcg aaactgatgg gcaacgtgcc
    18601 cgccactgcc agacaatctt ccagcgcaaa agggcggcag ggaattttca gggcgtgaaa
    18661 ttcccaggga agcacccctt cgccgataaa atgattgatt cctgcaagaa aggcatgcat
    18721 ggaagacagc atttcttcgg gaaacagctc ttccggatgc gccgccacac gctgtgccag
    18781 atcgcctacg cccatcagtc ggatgaggca gtccgccttt accagtggcg gccccagaat
    18841 ctcggacagc tgaccatgcg ccattcgccg gaagcattcc atctgaaaaa gccggtcctg
    18901 cgccatacag tagcccaggg caaaatgagc atccgcttcc gtggcggcgc ggatatgcgg
    18961 gatgccccat tcatcgcgca gcactgttac gctgttgtcc agcggacatc ccgccacagt
    19021 accgtcccag tccggcaggc accggtaact ccaggatact gtctgataca catgaacgga
    19081 cagcagtacc gcagcgattc cggtcaggag cagcagccct gctaaaaagc gacgtatcca
    19141 tttccacatc acagccatgg catgcccttc ccagcgtgtg agtttcaata cccgcgttat
    19201 ttcacgtccg cccggcagta ctcaggcact gagcaccgtc tccgaggctt ttccgttaga
    19261 ccgatccgaa gccttattca gtttcgtttc atgcaaagaa aggcagggaa ggccgatggg
    19321 tcaggatttc gaacaaaacc gcccatactg gcgaagcagt tccgcatcat ccatcttccc
    19381 tataagaagc cgggtagttg tgattgcctt ttcacctgct tttatcgtcc gcccgaaaag
    19441 cgaaaaatac atgctgaaat gagcgtcacc gtcctggggc gccataacag caaagcagtc
    19501 ctccgcccgt gccatgagag caacggtcag cccggttgcc tcgtgtcgtc tgaaaatcag
    19561 cggatgggca tagtccgccg ggatcgtcca atccaccgga ctgggaggaa tcgtccagcg
    19621 gccgtcacgg atcagggaaa gccctcggct ttctcccgga aaagcctgcc aggcacctgc
    19681 tgccggatcc gccttcagga aagaaggttc accccccggt ttttttacga agatctgtgc
    19741 cgacgggaac ccttctgtca tgtaggagga aagaaaaacc tcaaagtcgg gaaggtcttc
    19801 cacagcttcc acaatcgttt caacgtccag aatgcctgct tccacccagt ggtaaatgcc
    19861 gcgaagaaca taaggccgcc cttcagccgc ctgccagcga acttcaaggc aatcccgtcc
    19921 gtgcagctcc gtttctgcag gaagggcgcg catgctctcc acataacggt ggttggtgga
    19981 gaaaatccgg taataactga acagcccgta ggg
//
LOCUS       DAOPWB010000053        19668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2786_length_19668_cov_5.592515, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000053 DAOPWB010000000
VERSION     DAOPWB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 19668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19668
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2786_length_19668_cov_5.592515"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..139)
                     /locus_tag="PKN92_06640"
     tRNA            complement(50..139)
                     /locus_tag="PKN92_06640"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Ser,seq:cga)
     gene            358..1446
                     /gene="serC"
                     /locus_tag="PKN92_06645"
     CDS             358..1446
                     /gene="serC"
                     /locus_tag="PKN92_06645"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNZ48508.1"
                     /translation="MSTRNYNFSAGPATLPLPVLEQAKEEFLSYPGAGASIMEISHRS
                     KVFLKVLEEAKNNIKELLGMSDDYCILFTPGGATMQFSMLAMNFLNGKTADYLHVGSW
                     ASKAIESASRFGTVRKAWSGKAENFVRLPKDSELDLDPNAAYVHFTSNETIQGIQFLQ
                     EPDVKGKDLFCDASSDFLCRPTDISKYALLYAGAQKNIGPSGTAVVILRKGLLDRVPE
                     NLPPLLDYKVLADNDSLYNTPSTFTIYMIGLVSRWLLQTVGGLDKMFALNQEKAALLY
                     DTIDKSQGYYKGHAVPENRSIMNVAFRLGSEELEKKFLAEATAAGLDGLKGHRSVGGC
                     RASIYNAMPAEGVQALVDFMLEFQRTNG"
     gene            1564..1941
                     /locus_tag="PKN92_06650"
     CDS             1564..1941
                     /locus_tag="PKN92_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNZ48509.1"
                     /translation="MKSIAIVGASSDRKKYGNKAVRAFVKGGWKVFPVNKSGGTIEGL
                     DVFKSLDEIQEKIDRVSMYLPPSLGIGLLEEVVRKMPCEFYLNPGSESEALIAQAKAL
                     GLQPVQACSIVNIGLRPDMFPDE"
     gene            1987..2928
                     /locus_tag="PKN92_06655"
     CDS             1987..2928
                     /locus_tag="PKN92_06655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNZ48510.1"
                     /translation="MLLDIGCATHIGLKKTKNEDSFGIFDPKTAEVSLFGEGTLAAVA
                     DGLGGHIGGDIASKLAVSMLKDALKKEAPRDEDRGSEREDAHFLDAIKDAIKRANESI
                     HQTNKDVIQGKRPMGTTLCAALLRPHTAYVANVGDSRAELFRDGIFVRHTVDHSWIDE
                     QVQQGRMTQEEAEKDKRKNLLTRSIGTQPSVEVDTYQWPIRNGDQLLISTDGLLNMVN
                     DETRQQILTLDRTAQEKVDILIEKALENGGKDNVTIILAVVNPPKSKLRKQRRNAWFR
                     KHSANIRRKIVLFFYSLLLITLGYIAGYVHARFLSLF"
     gene            3103..4713
                     /locus_tag="PKN92_06660"
     CDS             3103..4713
                     /locus_tag="PKN92_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNZ48511.1"
                     /translation="MRDYCRTNVVASRKVQTPAKRMLFHFFVCALVFSFSLAAFAELQ
                     QVSVGGEIRIRGRYYINAWEEQRPLPRRIADALLPWRPIGPTGTMSRFKWDSKGKDWT
                     RYETSVLLNFKADFTEDVSAFFELYDFHIWGEEFRSNYLTGADSRANNVDFTLMNQAY
                     VEMRNLFNQPLQLRIGAQNLLFGGGWLLSNMMTPSQYISFDAIRATYSPGDFTIDAFA
                     AKLNDSDRVFKESVNLYGIYGTYNGIPEATMSLYWLYLRDDTDIPRPERTGWGRWVNN
                     KRGLQYGSTNLHTLGYKLSGGTGNLDYNLDLAYQWGDAEHLGSLFKPVGRAFGDTNAK
                     YDNYAVDATLGYTFKEVSWQPRFFLQGVVFSGDDNRDISFWDWVNPFYRPQASVAFNR
                     LFTEKNYMPTVNDNSWLSNFIQASAGVEVQPTEKLRLHFHVAKDWVYSPFNPPKSMRV
                     FGKKMYVAPRLSFWTDEGSRDLGWEVVAFARYNYSPDLWFMLYGNYLWTDDALTKGSF
                     IHFYGTEYSGGSNDKNAGYLFWMAVLKF"
     gene            complement(4994..5773)
                     /locus_tag="PKN92_06665"
     CDS             complement(4994..5773)
                     /locus_tag="PKN92_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ48512.1"
                     /translation="MSKTVLAVGAHPDDIEFMMAGTLLQLAAKGWEPHIFVVASGSCG
                     STITDAKETAEIRYAEAAEAAQKLGAHFHPPVVDDLEIIYSLPLLGKIGAIIRKVNPE
                     IILTHPPTDYMEDHEGTCRLTVSAAFARGMKNFETDPPVDAVEGDTTVYHCLPYGLRD
                     SLHRPVPADFFVDIANCLDRKKDALACHRSQKVWLDQSQGIDSYLNNMVELSKKMGEE
                     SRVFTYAEGWLKHNPLGFCNQTANPLAQALGSLLVSASPAI"
     gene            6114..6425
                     /locus_tag="PKN92_06670"
     CDS             6114..6425
                     /locus_tag="PKN92_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48513.1"
                     /translation="MNSLIDKLTAMESEAAALIEQAKAKAASLDKKADEQIESIKKEM
                     SAVLDQKILAHREEVQKKHDALAAAQDSTERKALAALDSISSDALAVQADKIVARFRE
                     I"
     gene            6428..8488
                     /locus_tag="PKN92_06675"
     CDS             6428..8488
                     /locus_tag="PKN92_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNZ48514.1"
                     /translation="MAIDIMKKATVLCPLTSTKQLLKSLHAEGIIELVDVMERYEEAR
                     DALSRPSVSTADCDDELQKLSLIIGLLNTFAPEEKSFIQGLAPVPLLVDPDELNQLYQ
                     QFPLQKHYEHALDLDGEYRKIERAHGATENRLAELAPLAHFPYALEQLTRLQEVRLIL
                     GSISLKNLNTLKQTPEKPAGMHWEEIDPQGAVHDNSDPKNKAGKAPEVVWLLVAVLPD
                     AETDARQVLNAHGFLEIALPQISGTVAEQIRSLEEDLVVQKQKLENVAGQVREMAQYR
                     HEFLVLRAGWEDRKKLKLSRLQSAHGEYVQVLTGFIRATDSPRLEEMLRHDFSEASLL
                     LEDVTDEDAVPVSITLPKLVRPVQLLVDLFGRPTYETFDPSPFIMPTFLLFFGICFSD
                     VAYGAMLSLLSLYIMKKTKPYVGIYNFAKLLLYGGLSTMFFGFVLGGFFGDLYMPQYM
                     GENNLLYRFMSSVQIISPLDKPIAVLLISLAIGMANQFYGIGLQMYSSMLRGDKKAVL
                     YDGLLWLVALPGFVLIIGKMFAPVPAPLFYLGMLLFFGGAIGLVLTQGRENEGLVQRF
                     LTGLVSLYGIVGSYGITAFIGDTMSYCRLLALALTTGIVALSFNMMAGLLRPIPYVGF
                     ILFIVVLIAAHVFNFFINVLGAFVHSMRLIFVEFFGRFYASGSKTFTPLGFNSKTAVL
                     YKKQ"
     gene            8568..9077
                     /locus_tag="PKN92_06680"
     CDS             8568..9077
                     /locus_tag="PKN92_06680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNZ48515.1"
                     /translation="MDHALAVEIGRGLAIAGAGIAVGLGCCGSGKGIGIASQAAAGVI
                     SEKPDLFGQLLVMLALPGTQGFYAFITGIMIFMRTGLIAGEWTASVSTGVALFFVGLG
                     VGFAQYVTAIWQGEASAAGIALVARRQEESGRALLFPAMVETYAVVALLAGILSIIWL
                     TNPNFVAFN"
     gene            9117..9719
                     /locus_tag="PKN92_06685"
     CDS             9117..9719
                     /locus_tag="PKN92_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNZ48516.1"
                     /translation="MALNEITQAVLDRARKNAELIVKSAQVASEEKVEAWRKNAETEM
                     DKKFQSIKLGIEEEFSRKLIQMQGISNKAVLTKKNELLQQVFALAKAKILSMPREEYR
                     KVFKRLLEKSVGQSSGALKIHPEDKAVFEEMLAEFNENRDEKDHVRLLDEESLPERGG
                     FIFVTDRFQVDQSLSTLLSDVQYESAPEISAALFSDDSGK"
     gene            9735..10730
                     /locus_tag="PKN92_06690"
     CDS             9735..10730
                     /locus_tag="PKN92_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNZ48517.1"
                     /translation="MAALTKNQNKWGFACGRVSALEGQLIPQETFYLLAGLDRVQDVF
                     NRLQETSLREHMTHGALTWEDWSTVIDNYVHSQILSLRQCTPNPAICDLYSLSEDYLN
                     LKRAVQNRGSFPFGKSQFSESRLLEVASGNGSLLPEIIRPALSPVLGGGSQEENSQFV
                     LDVTLDGAFLRHYLFLAHQSEAPFIEEWATLRVLGRALVAMWRAFRAGQQLKVFPAHF
                     LPLNSLDGLITEISTIPDPKAWGASIPGRPGDLWQAAFEAEEDEQVSTFETLLANHLT
                     ELAQRVKLQTAGPERVAGYLWGLWIAAYNLKLVISGKLNNLDENLLKKRIRNTYV"
     gene            10723..11043
                     /locus_tag="PKN92_06695"
     CDS             10723..11043
                     /locus_tag="PKN92_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNZ48518.1"
                     /translation="MSKAYILGDKAAVLGFKGVGFEIVHAYDSETLIQALGQVIRAND
                     AAVVLVSEDLAEKNPEALANFRENCNAIMTTIPTHHGSNHSGFKEIRRLVEHSIGVDM
                     LGKE"
     gene            11089..12846
                     /locus_tag="PKN92_06700"
                     /pseudo
     CDS             11089..12846
                     /locus_tag="PKN92_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
     gene            12893..14272
                     /locus_tag="PKN92_06705"
     CDS             12893..14272
                     /locus_tag="PKN92_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNZ48519.1"
                     /translation="MVTREYRTAKEIVGPLVLVDGVEGVTFGELTDIKMDDGTLRRGR
                     VLEVNGDKAVVQVFEGTSDLSPENLTIKFLGKGTELGVSDDMLGRVFDGFGRPIDDGP
                     PIIPEKWLNINGNPMNPYSRDYPNEFIQTGISTIDLLNTLVRGQKLPFFSGSGLPHSR
                     MAAQVARQAKVLKADDKFAVVFGAMGITFEESEFFVSDFRRTGALERAVLFMNLADDP
                     AIERISVPRMALTTAEFLAFEKGMHVLVILTDLTNYCEALREISAARKEVPGRRGYPG
                     YLYTDLSTIYERAGRIKGKNGSITQLPVLTMPEDDKTHPIPDLTGYITEGQIIMSRSL
                     HAQGIYPPVDVLPSLSRLKDKGIGAGKTRADHANVMNQLYSAYARGKNAKELAVVLGE
                     SALSEIDLLYVKFADAFENRFIRQGEDENRSIEDSLVLGWELMAILPKSELKRVRDEF
                     IEKYYPEKG"
     gene            14306..14935
                     /locus_tag="PKN92_06710"
     CDS             14306..14935
                     /locus_tag="PKN92_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNZ48520.1"
                     /translation="MRLAVNPTRMEMLRLRNRLSVAQRGHKLLKDKLDGLMKDFGEYA
                     QKYKECRLAVDAELPVVLKLFVLAEATSSRLIVEDALELTRQELDITSIPTRIMSVVI
                     PRLEFSFGKSLGGYSLIHTSAELDKAISGLKVFMEKLLTMAELEETVRRLAQEIEKTR
                     RRVNALEHTVIPRLLETIKFIKNKLDEMERSTTSRLMKIKEQRLAQEGK"
     gene            15017..16345
                     /locus_tag="PKN92_06715"
     CDS             15017..16345
                     /locus_tag="PKN92_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48521.1"
                     /translation="MAADLRLDSFDADERKAALLEVYRSALSTDSLPAFGRHFNLHCH
                     SFFSFNGYGFSPTHLAWQGRLLGLCAMAQVDFDVLDGVDEFLSACRFLDIKAAAGMES
                     RVFVPEFSEEEINSPGEPGVAYHIGLGFASSKVKETALLHSFKDKAQSRTRVVVQKVN
                     TLLHEIALDYDQDVLSLTPAGNATERHVCAAYDRKARNYFTEPSRLISWWSEKLDLAK
                     EKIEEALSSPPDFQGIIRAKTMKQGGVGYIASKGEDFPTLRVVNQFFLDNGAIPVLAF
                     LDGTSSGEKRMDDLLDIMIDSGVGAVNIIPDRNWNIKDPDVRALKISKLDEFIGMARK
                     RHLPIIVGTEMNAHGQALVDALDAPALAPYFKDFEEGMFFIYGHTVLASMAELGHSSA
                     WSQASFSTAAERNAFYIQVGRLASPAIEGNTLGISSSDTPEIILKKFSRE"
     gene            complement(16332..17207)
                     /gene="murB"
                     /locus_tag="PKN92_06720"
     CDS             complement(16332..17207)
                     /gene="murB"
                     /locus_tag="PKN92_06720"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNZ48522.1"
                     /translation="MKLSPPAFIKENLSLAPHCFYNIGGTMRWALFPRSLEEIQEGYQ
                     WIQKKGVPFLILGGGTNVLVSDKGYDGAVLFTGGLDYSTPLGCNRFKVGAGTILASLV
                     TQVLLPNNYTGTGALTGIPGSVGGALFMNAGTVNGTICQFTESVQLLSASGLTQWQMS
                     SDDYCYRGQRFCTTNTVILEGVFSFTVDEKDQLAVYEHYLKRRKETQPQGRCCGSVFK
                     NPEGDHAGRLIELCGLKGTRRGGAVISEKHANFIVNQDNASFDDVYGLICLAKEEVLK
                     RFGIELKEEVRIIHG"
     gene            complement(17239..18330)
                     /locus_tag="PKN92_06725"
     CDS             complement(17239..18330)
                     /locus_tag="PKN92_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48523.1"
                     /translation="MKVGIYMPFGERGKSASSLFYDGLEKEYFRRALGAVHAQKAYSD
                     MELLWFDEGGNPSPCAFVRTTAIEPVRSSLSLFKSSSRLLEKVVQDEGLDVLLTHIST
                     PLLRLSIPQILFTADMLFHGETRSVSNTPPPPLSSKIKRACMSAQSILCPSEYVHRSC
                     LSRLEMGLEKAIVAKAGVDKIFSEPSPSLIDPPYGLFILNRYTFPALPTFFNAIQKNT
                     SLFPQNLVVLGPVHPEEPSNWPLPVVRIENCPDKEVAGLMQNAEMCFYLAHGDGSGIP
                     VLQALSSGALLITTRSGAINEVAGSVPFYCEADNITSLLNILRRAKNEKASDLRKRKQ
                     TAQNLIHDCSWERCGTKILSTLKRSQLNS"
     gene            complement(18416..19567)
                     /gene="sat"
                     /locus_tag="PKN92_06730"
     CDS             complement(18416..19567)
                     /gene="sat"
                     /locus_tag="PKN92_06730"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HNZ48524.1"
                     /translation="MSIAPHGGELKNCFIEAGDLESFKRHAASLPVIQIDAYTAFDVD
                     AIAKGIFSPLEGFLGKEDTLSVLESMCLKTGLPWTIPVLFSVDTAVADTLELGQEVAL
                     KDDYNQIIAVMNIAEKFQLDKKYLAEKVYRTTETAHPGVAYTFSTGDVYLAGQLKVAH
                     SRPVDHQEYNLSPLETRAAFKERGWKRIVAFQTRNPIHRAHEYLTKCALEMCDGLLIH
                     PLMGTTKSDDIPGDIRMKCYEVLLDKYYPKEHVMLSIMPVNMRYAGPREAVMHAIIRK
                     NYGCTHFIVGRDHAGVGNYYGSYDAHYIFDEIDPAALGITPLFFEHAFFSKRTNDMAT
                     KKTCPGSSEEHVFLSGTKVRELLANGEAPPVEFTRPEVAQILIEWATGK"
BASE COUNT         5028 a         4517 c         4818 g         5305 t
ORIGIN      
        1 agggaaaatc ggctggcgaa ttctcgccaa ttccccccag tttcctgaat ggcggagagg
       61 gtgggattcg aacccacggt acggtaaacc gtacaacggc tttcgaggcc gccgccttca
      121 accactcggc cacctctccg ctggaaggca tattgtagca gaaaaagaga gaaggagtca
      181 tcgtgagggc atcggtactt agtttattgg tgtttacatt cataaaaccg tataatatgc
      241 cccagcgttt gcatgcaaac gctccctttt ccctccggat ggaacttcac aactctgttc
      301 cggtttcctt ctttcaccgc ttttttaggc aacgcaactg cttcaaaagg agtttttatg
      361 tcgacgcgca actataattt ttctgcgggt cccgccacac ttcctcttcc cgttttggaa
      421 caagcgaaag aagaattctt gtcatatccc ggagccggtg cttccatcat ggagatcagc
      481 caccgttcca aagtctttct gaaagttctg gaagaggcaa aaaataatat taaagaactg
      541 ctgggcatgt ccgatgatta ctgtattctt ttcacaccgg gcggtgccac catgcaattc
      601 agcatgctgg ccatgaattt tttgaatgga aaaacagctg actatctgca tgtcggctcc
      661 tgggcatcca aggccattga atcggcgagc cgtttcggca ctgtccgcaa ggcgtggagc
      721 ggtaaagcag aaaattttgt gcgcctgccc aaggattctg aattagatct ggatcccaat
      781 gcggcgtatg ttcattttac gtccaatgaa acaatccagg gaattcagtt tttacaggaa
      841 ccggatgtta aaggaaaaga ccttttctgc gatgcctctt ctgacttttt gtgcaggccc
      901 accgatatca gtaaatacgc gctgctctac gcaggcgccc agaaaaatat cgggccttcc
      961 ggcacagcgg ttgtgatact tcgaaaaggt ttgttggatc gtgtcccgga aaaccttccg
     1021 cccctgttgg attataaagt tcttgctgac aacgactcac tttataacac gccttcaact
     1081 tttactatct acatgattgg ccttgtgagc cggtggcttc ttcaaaccgt tggcggtctg
     1141 gataaaatgt ttgctttgaa tcaagaaaaa gctgcattgc tttatgatac catcgacaaa
     1201 agtcagggtt actataaagg gcacgctgtt cctgaaaacc gttcgattat gaatgtcgct
     1261 tttagactgg gctccgaaga actggaaaag aaatttcttg ccgaagcgac agctgccggt
     1321 ttggatggct taaaagggca ccgttcagta ggcgggtgcc gtgcttccat ctataatgcg
     1381 atgcctgcgg aaggcgttca ggcgcttgtt gattttatgc ttgaatttca gcgcaccaat
     1441 ggctgactat tggaaaggtc ttgatgcggg atattcatag aacgcgggta tcccgctcct
     1501 tcaacatagt ccgataaacg gcttgcctgt tgctaagggg tctgtacgca ttaggagaac
     1561 acgatgaaaa gtatagctat tgtaggagcg tcgtccgatc ggaagaagta tggaaacaaa
     1621 gctgtacgcg cttttgtaaa aggtggctgg aaagtgtttc cggtcaataa gtccggtggc
     1681 actattgaag gtcttgatgt ttttaagagc cttgatgaga ttcaggaaaa aatagaccgt
     1741 gtttccatgt accttccccc ctcactgggc attgggcttc ttgaggaagt ggtgcgcaaa
     1801 atgccctgcg agttctacct gaatcccggg tcagaaagtg aagcattgat tgcacaggca
     1861 aaagcgctgg ggcttcaacc ggttcaggca tgcagcattg tcaatatcgg gctccgtcct
     1921 gacatgtttc ctgatgaata aaacaaggtc cggtgcagag ggcaaaacct gaagagtgaa
     1981 tgcgtcatgc tgttagatat cggttgcgct acgcatatag gtttgaagaa aaccaaaaat
     2041 gaagacagct tcgggatctt tgacccgaaa actgctgaag taagcctttt tggtgaagga
     2101 acccttgcag ctgttgccga tgggttgggt gggcatattg gcggcgatat cgcatcgaaa
     2161 cttgctgtgt ctatgttgaa agatgcattg aaaaaggagg cgccccggga cgaagatcgc
     2221 gggtcggagc gtgaagatgc acactttctg gatgccatca aagatgccat taagcgcgca
     2281 aatgaaagca ttcaccagac caataaagat gttattcagg gaaagcgccc catgggcacg
     2341 accttatgtg ctgccctgct ccggccccat actgcttatg ttgccaatgt gggcgactca
     2401 agagcagaac ttttcagaga cggtattttt gtgcggcaca ccgtggatca cagttggatt
     2461 gatgagcagg tgcagcaggg ccggatgacc caagaggaag cagaaaaaga caaaagaaaa
     2521 aatctgctta cccgaagcat cggcacccag ccgtccgttg aagtggatac ttaccaatgg
     2581 ccgatcagaa atggcgatca gcttcttatc tctaccgacg gtctgctgaa tatggtaaac
     2641 gatgagacac gccagcagat acttactctt gatcgtacgg ctcaagaaaa agtagatatc
     2701 ctcatcgaaa aagcacttga aaacggcggg aaagataatg tgacgattat tctcgctgtt
     2761 gtcaatccgc caaaatccaa acttagaaag cagcggcgta acgcatggtt cagaaaacat
     2821 tcggctaaca ttcggcgaaa aattgtcctc tttttttaca gtctccttct tataacgttg
     2881 ggatatatag cgggctatgt gcatgcccgc ttcctttccc tcttttgaat tctccttaag
     2941 atttcagcct aaattctatt cttataaaaa ttcgcatccc tttttttttc atgttataat
     3001 ggcagtaaca cataaaaatg ctaaaactct gtatcacgtc cagagttgcg agtcataagg
     3061 aaacaactgg tttttataag tcaaaaaaag gaggaagttt tcatgcgtga ttactgccgg
     3121 acgaatgttg ttgcgtccag aaaagtacaa acaccagcaa agcggatgct ctttcatttt
     3181 ttcgtttgtg ctcttgtttt ttctttttct cttgctgcat ttgccgagtt gcagcaagta
     3241 tccgttggcg gtgaaattcg gatacgcgga agatattaca taaatgcctg ggaggagcaa
     3301 cgtcctctcc cccgccgtat tgcggacgct cttctgccgt ggcgccctat cggccctaca
     3361 ggaacaatgt ccagatttaa atgggacagc aaaggcaaag actggacgcg ttatgaaact
     3421 tccgtgcttc tgaatttcaa agccgacttc acagaagatg tttctgcttt ttttgaactt
     3481 tacgatttcc atatctgggg tgaagaattc cgttccaact atctgacggg ggcagacagt
     3541 cgcgccaaca atgtcgattt tacgttgatg aatcaggcat atgtggagat gcgtaattta
     3601 ttcaaccagc ccctgcagct gcgtatcggc gcgcagaatc ttctttttgg cggcggatgg
     3661 cttctttcaa atatgatgac gccgtcacaa tatatttctt tcgatgccat tcgggctact
     3721 tattcgcccg gcgattttac cattgatgct ttcgcagcca aattaaatga cagcgacaga
     3781 gtttttaaag agtcagtaaa tctttacgga atttatggaa catacaacgg cattcctgaa
     3841 gccaccatgt ctctttactg gctatatctc cgggatgata cggatattcc tcgacccgaa
     3901 agaaccggtt ggggacgctg ggtaaacaac aagcgcgggc tccaatatgg ctcgacgaac
     3961 ctgcataccc tgggctacaa actttccggg gggacgggca acctggatta taatctggat
     4021 cttgcctacc agtggggaga tgctgaacat ctgggttctt tgtttaaacc ggtaggcaga
     4081 gccttcggtg ataccaatgc caaatatgac aactacgctg tagatgcaac gttggggtat
     4141 acgtttaaag aagtttcctg gcagccccgc tttttccttc aaggcgttgt ttttagcggt
     4201 gatgataata gggatatcag tttctgggat tgggttaatc cattctaccg cccacaggct
     4261 agcgttgctt ttaacaggct ctttaccgaa aaaaattata tgcctactgt caatgacaac
     4321 agctggctct ccaattttat acaggcctca gcaggggtag aagtgcagcc gactgagaaa
     4381 ttaagactcc atttccatgt tgccaaagac tgggtctatt ctcccttcaa cccgccaaaa
     4441 tcaatgcgtg ttttcggcaa aaagatgtat gttgcgccca gactgagctt ctggacggac
     4501 gaaggcagcc gggacctggg ctgggaagtt gtggctttcg cacgatacaa ctattctcct
     4561 gacctgtggt tcatgcttta cggaaattat ctgtggacgg atgatgcttt gacgaaaggc
     4621 tcttttatcc acttctatgg tactgagtat agcggcggtt cgaatgataa aaatgccggc
     4681 tatcttttct ggatggccgt tttgaagttc taaataaagc cttcagattc ttttgcgcgc
     4741 tgtgtttgca atacgcagcg cgcttttttt tgtaaagtta tccccaactt atccacacct
     4801 cacacaggag cacaattcct agtatttttg taaaagaagt ttctatctca tgtattttca
     4861 ataagatata ttttatatgt tgaaattatc agtattgcgc aatcaatctg cgcaaagaaa
     4921 aggaaagtta ttcacgtctt gcagaaatgg tttcactgca catgcgattg ataggaaact
     4981 tccaaaaaag tatttaaatc gcaggagatg cggaaacaag aagggaaccg agtgcctgcg
     5041 cgagagggtt ggctgtctga ttgcaaaagc caagagggtt atgcttaagc cacccttcag
     5101 cataggtgaa aacgcggctt tcttccccca tttttttgct tagttccacc atattattca
     5161 agtaactgtc gatcccctgg ctttggtcga gccatacttt ttggctgcga tggcaggcaa
     5221 gcgcatcttt tttacgatca agacagtttg cgatatcaac gaaaaaatca gccggcaccg
     5281 gtcgatgtaa agaatctctc agaccatagg gcaaacaatg gtagacggtt gtgtctcctt
     5341 ctactgcatc tactggcggg tccgtttcga agttcttcat tccccgtgcg aaggctgcgg
     5401 aaacggttaa tctgcaggtg ccttcatggt cttccatata atcagtgggt ggatgagtca
     5461 gtataatttc agggttcact tttcgaataa tggcacctat cttgcccagt aaaggaagag
     5521 aataaatgat ttcgagatca tccactaccg gagggtgaaa atgagcgccg agtttttggg
     5581 ccgcctcggc agcctccgca tagcggattt cagctgtttc tttcgcatcg gttatcgtgc
     5641 tgccacaact gccagacgca actacaaaaa tgtgaggctc ccaaccttta gcggccaact
     5701 gcaaaagtgt tcctgccatc ataaattcta tatcgtccgg atgagcacct acggccaata
     5761 cggttttaga catgttcaat tgctcccagc ggttgttttg attatagtat aatttttttt
     5821 atagtaagaa caaacccacc gtttctattg tttttttgcg gtctacgact ccctctttgt
     5881 gaaaggcaaa taataataaa aagtctggag aaaaagattt ttaaattgca actaatgacg
     5941 cttgcattta ccgggtactt tttttatata attgtattgc agaattcttt tgcagtagtg
     6001 gaccacggtc aacagattca catcctgcta attcaaattt tgggtatatt tttttaatac
     6061 ctaccgttcg gtataattgg tcctgtgcag tttttaaacc acggataatg catatgaact
     6121 ctttaattga caaactgact gcgatggaaa gcgaagcggc tgccttaatt gagcaggcta
     6181 aggcgaaagc ggcgtcgctt gataaaaaag cggacgagca gatcgaaagc attaaaaaag
     6241 aaatgagtgc tgtgcttgat cagaagattc tggctcaccg tgaagaggtt cagaaaaagc
     6301 acgacgccct ggcagcagcg caggacagca cggaacgcaa agccctggca gctctggaca
     6361 gcatttcttc agacgctttg gctgtacaag cagacaaaat tgtagcacga ttccgcgaga
     6421 tataattatg gccatagaca taatgaaaaa agccacagtg ctttgtccgc ttacgtcgac
     6481 caagcagttg ctcaaatccc ttcacgccga gggtattatc gaattggtcg atgtgatgga
     6541 gcgctacgaa gaagcgcggg atgcattatc acgcccttct gtttccacgg cagactgtga
     6601 tgatgaactg caaaaattat ccctcattat agggctttta aatacatttg cgcccgagga
     6661 aaagagtttt attcaagggc ttgcgccggt accccttctt gtggatcccg atgaattaaa
     6721 tcagttatac cagcaatttc ctctgcagaa gcattacgaa catgcgctcg atctggacgg
     6781 agagtaccgc aaaatagagc gtgctcacgg cgcgaccgaa aaccggctgg cagagttggc
     6841 ccctcttgct cattttccat acgccctgga acagttgaca cgccttcagg aagtccgtct
     6901 tatactcggc tcaatttccc tgaaaaattt aaatacttta aagcagactc ctgagaaacc
     6961 ggcgggaatg cactgggaag aaattgatcc gcaaggcgcg gttcatgata actcggaccc
     7021 gaaaaacaag gcaggaaaag cgccggaagt tgtctggctt cttgttgctg tcttgccgga
     7081 cgctgaaacg gacgcacgac aagtactgaa tgcacatggc ttcctggaga ttgcattgcc
     7141 gcaaatttcc ggtactgtcg ccgagcagat ccgctcactg gaagaagacc tggttgtaca
     7201 gaaacagaag ttggaaaatg ttgcaggtca agtccgggag atggcgcaat accgccatga
     7261 atttttggtg cttcgggccg gctgggaaga ccgtaagaaa ctcaaattaa gtcgtctcca
     7321 aagcgcacat ggagagtatg tgcaggtgct cacaggcttc atccgtgcaa cggacagccc
     7381 ccgactggaa gaaatgctgc gccatgactt ttccgaagcc agccttctgc tggaagatgt
     7441 gacagatgag gacgcagtgc ccgtcagtat caccctgccg aaactggttc gcccggtaca
     7501 gcttctggtt gatcttttcg gcagacctac ctacgagacc tttgacccgt ctccgttcat
     7561 catgcctacc tttcttcttt tcttcggtat ttgttttagt gatgttgctt atggtgccat
     7621 gctttcactg ctaagcctct atatcatgaa aaaaacaaag ccttatgtcg gcatatacaa
     7681 ttttgcgaag ctccttcttt acgggggctt gtcaacgatg tttttcggtt ttgtgctggg
     7741 cggttttttt ggcgatttat acatgcccca gtatatgggt gaaaacaatc ttctttatcg
     7801 ctttatgagc agtgtacaga taataagtcc cttggataaa cccattgcag tgctgttaat
     7861 ttctcttgcg atagggatgg caaaccagtt ttacggtatc ggtctgcaga tgtattcttc
     7921 catgttgcga ggagataaga aagccgtttt atacgatgga cttctatggc ttgttgcgct
     7981 gccgggtttt gttcttatta tagggaaaat gtttgcacct gtgcctgctc ccctctttta
     8041 tctgggtatg ctcctctttt ttggaggtgc cattggtctg gttcttacac agggcagaga
     8101 gaacgaaggg cttgttcaac gatttttaac agggctggtg agtctttatg gcattgtcgg
     8161 ctcctacgga attactgcct ttatcggtga caccatgtcc tactgccgtc tccttgccct
     8221 tgcactgacg accggcattg ttgcgctttc atttaatatg atggcagggc ttttacgtcc
     8281 cataccttat gtcggcttta tattatttat agttgtgctt attgctgctc acgtatttaa
     8341 tttctttatt aatgtactgg gcgcttttgt gcattcgatg cgtcttattt ttgtggagtt
     8401 tttcggtcgg ttttacgctt ccggatcaaa aacctttacg cctctgggat ttaattccaa
     8461 aacggcagtg ctttataaaa agcagtaaag ggcttaaatg ttctggatcg tttgatcatt
     8521 ttgtaaagta ttcggtgtaa aaacaacagt agtattggag agtaaccatg gatcatgcac
     8581 tagcagttga aattggacgc gggttagcca tcgcaggtgc gggtattgcc gttggactgg
     8641 gttgctgcgg ctccggaaaa ggcattggca ttgcatccca ggccgcagcc ggagttatca
     8701 gtgaaaagcc tgatttgttc gggcagcttc ttgtgatgct tgcacttccc ggcactcagg
     8761 gcttttatgc cttcattacc ggcatcatga ttttcatgcg cacaggtttg atcgccggcg
     8821 agtggaccgc ttctgtcagc accggtgttg ccctgttctt tgtcggcctc ggtgtcgggt
     8881 ttgcacagta tgtgacagct atctggcagg gagaagcttc tgctgccggt attgcactcg
     8941 ttgcccgtcg tcaggaagag tccgggcgtg cgcttctttt ccccgccatg gtggaaacct
     9001 atgctgtggt tgctcttctt gccggtattc tttccattat ttggctgacc aatcccaatt
     9061 ttgttgcttt caattgaccc gttatgtttt taagtaaatt acagggatga cagaccatgg
     9121 cactcaatga aattacgcag gccgtccttg atcgggcacg caaaaacgcc gagctgattg
     9181 ttaaatcggc gcaagttgcg tcggaagaaa aagtagaagc ctggcgtaaa aacgctgaaa
     9241 cagagatgga caagaaattt cagagcataa aacttggcat cgaagaagag ttttcccgca
     9301 aactcataca gatgcaggga atttcaaata aagcagttct aacaaagaaa aatgagttgc
     9361 tgcagcaggt ttttgccctg gcaaaagcga aaattctgtc catgccccgg gaagaataca
     9421 ggaaggtatt taaaagactt cttgaaaaat ctgtcggcca atcaagcggt gcattaaaaa
     9481 ttcatcctga ggacaaagct gtttttgaag aaatgctggc cgagttcaat gagaaccgcg
     9541 acgaaaagga tcatgttcgg cttcttgatg aagagtccct tcctgagcgt ggcggtttca
     9601 tttttgtaac tgaccgtttt caagtggatc aaagcctgag cacgctttta agcgatgttc
     9661 agtatgagtc tgcgccagaa atttcagccg cactcttttc ggatgattcc gggaaatagt
     9721 gagaggaacc caatttggca gccttgacta aaaaccagaa taaatgggga tttgcctgcg
     9781 ggcgtgtcag tgctttagaa gggcagctga ttcctcagga aaccttctat ctcctggcag
     9841 gtttagaccg tgtccaggat gtttttaacc gactgcagga aacatcactg cgtgagcata
     9901 tgacccacgg agcgctgacc tgggaagatt ggagcacagt aatcgataat tatgtgcact
     9961 cccagattct ttcactcagg cagtgcaccc ccaatccggc aatctgcgat ctctacagcc
    10021 tgtccgaaga ttatctgaac ttgaaaaggg ctgttcagaa tcgcggttcc tttccatttg
    10081 gaaaaagcca gttttccgag tccagactgc tggaagtcgc gtccggcaat ggaagcctcc
    10141 tccctgaaat tatccggcct gcgctctccc ctgtcctggg aggcggcagc caggaagaaa
    10201 acagtcaatt tgtgctggat gttactttgg atggtgcttt tttgcgtcat tatctttttc
    10261 ttgcgcacca gtccgaagcc ccttttattg aagagtgggc aacgttaagg gttctcggca
    10321 gggctctggt cgccatgtgg cgcgctttca gagcgggaca gcagttgaaa gtctttcccg
    10381 cacatttcct gcctttaaac agtctcgacg ggcttatcac tgaaatcagt accattcccg
    10441 atccgaaagc ctggggggca tccattcccg gccggcccgg tgatttgtgg caggcggctt
    10501 ttgaggcaga agaagacgaa caagtgtcca cattcgagac ccttcttgcg aatcatctga
    10561 cggagctggc gcagcgggta aaacttcaga ctgccggccc tgaacgggtc gcgggttatc
    10621 tttggggact ctggattgcc gcttacaatc tgaagcttgt tataagcgga aaactgaaca
    10681 accttgatga aaatcttctg aaaaagcgaa tacggaatac ttatgtctaa agcatacatt
    10741 ttaggtgata aagcggcagt gctcggtttc aaaggcgtag gctttgagat tgtgcatgcc
    10801 tatgactccg agactttaat acaggctctt gggcaagtga tccgtgcgaa tgatgctgct
    10861 gttgttctgg tgagtgaaga tcttgcggag aaaaatccgg aagcactcgc taatttccga
    10921 gaaaactgta atgccattat gaccaccata cccacccacc acggcagcaa tcactccgga
    10981 ttcaaagaga ttcgccgtct tgttgagcat tctatcggtg tggatatgct tggtaaagaa
    11041 tagtcagtat aaagaaagat acaagcagat agtacaaggg tatcaagtat gagcaatgta
    11101 cagggtgtag tcgtcaaagt gtccggtccg cttgtggttg caagaggtct ggaaaatgcc
    11161 cgcatgtatg agatggtgcg tgtgggcgaa ggtcgtctgt tcggtgaaat catcgaaatc
    11221 cgcagcggag actgctccat ccaagtctac gaagagacgg aagggatcgg ccccgggcag
    11281 cctgttttcc gtacggaaga ggctttgagt gtcgaattgg gtcccggcct tatccgttca
    11341 atttacgacg gtgtgcagcg tcctcttgac aaactgcatg cttccttcgg cgagtatatt
    11401 gttcgtggag ccgaaagccc tgctttggat cggtctgttc agtgggactt tgtgcctgtt
    11461 gccaaagtgg gtgatacagt gtctcatggc gacattctcg gtgaagttga cgagagcaag
    11521 cttgtcagtc acaaaattat ggttccttat ggtgtggaag gctaagtaag cagaattgcc
    11581 ccggtaacgg gcaatgtcga caccgtggtc gccgttatcg acacctctaa aggacccgtt
    11641 gatatcagca tggttcaacg ctggcctgtg cgtaagcccc ggcctgtcgc caagaaaatg
    11701 ccgcctgtcc agccgatgac caccgggcag cgtgttctgg atatgttttt cccgctgact
    11761 cagggcggca cagcctgtgt tcccggaccg tttggcagcg gcaaaactgt tgtgcagcat
    11821 cagttggcta aatgggcgaa tgcccagatc gttgtctatg tcggctgcgg tgagcgcggc
    11881 aatgaaatga cagacgttct tcaggagttc cctgccttga tcgaccccaa aacgggcgaa
    11941 ccgctgatgg aacggactgt gctcatcgcc aacacatcca atatgcctgt tgctgcccgt
    12001 gaagcctccg tgtttaccgg gatcaccatt gccgagtatt tccgtgatat gggctactcg
    12061 gttgcgctta tggcggactc gacaagccgc tgggcggaag cgatgcgtga aatgtccggt
    12121 cgtctggaag agatgcccgg agaagagggg tatcctgctt atctcagttc acgcattgcc
    12181 agtttttacg aacgcgcggg caacgtaatt tgtaaaggat ccgatgaaag aaacggcacg
    12241 ctctccatta tcggagccgt ttcgcctcca ggcggtgact tctccgagcc tgttgtgcag
    12301 gcaacgcttc gtgttgtaaa agtcttctgg gggcttgatg ataaacttgc ctttgcccgg
    12361 cacttccctg ctatcaactg gcttacttcc tactcccttt atcaggaagt agtagatgca
    12421 cttgccaatg agaagctgca gcagccctgg aaggaaaatc gcagtcaggc aatgagtatt
    12481 ctgcagcgcg aagcggaatt ggacgaactg gtgcgcctgg tcggtatgga tgctcttccg
    12541 cagtcagacc ggttgcttct ccaggccgcc aagatgatcc gtgaggactt tcttcatcag
    12601 aatgccttcg acgatcggga cacctatacc tccatggaaa aacagttcaa aatgctggac
    12661 ttgatcctga catattttaa cgaagccaga ccggctctgg aagccggagc cctgctgaac
    12721 agccttctca gtttgaaggt gcttgaggat attgcgcgtg caaaactgat cgaggaaaat
    12781 gctcttcatc agttcgacgc gattaaggat aaattaaccc gggaaatagc tgccctgccc
    12841 cgttaagcag atgcggaaga atgatcaaaa caacaatgaa tgaggtcgta ccgtggtaac
    12901 tagagaatac cgtacagcaa aagaaatagt aggacccctg gttcttgtag atggggttga
    12961 aggcgttact ttcggcgagc ttaccgatat taaaatggat gacggtaccc ttcgccgcgg
    13021 ccgtgtgctg gaagtaaatg gcgacaaagc ggtcgttcag gtcttcgagg gtaccagcga
    13081 tctttcccca gaaaatttga ccatcaaatt tttaggaaaa ggcacagagc ttggtgtttc
    13141 cgatgatatg cttgggcgtg tttttgatgg tttcggccgc cctattgatg acggaccgcc
    13201 cattattcct gaaaaatggc tgaacatcaa cggcaacccg atgaatcctt attcccgtga
    13261 ctatccgaat gagtttattc aaacgggtat ctcgaccatt gatcttttga atactctggt
    13321 tcgtggacag aaacttccat tcttttcagg ttcaggtctt cctcattccc gtatggcggc
    13381 tcaagtggcg cgtcaggcaa aagtattgaa agccgacgac aaatttgctg ttgttttcgg
    13441 tgccatggga atcacttttg aagaatcaga gttctttgtt tccgactttc ggcgtacggg
    13501 tgcacttgaa cgtgccgtgc tgttcatgaa tcttgctgat gaccccgcca tcgaacgtat
    13561 ctctgttccc cgaatggcgc ttacgaccgc tgaatttctt gcatttgaaa aagggatgca
    13621 cgttcttgtc atccttacag acctgaccaa ttattgcgag gcattgcgtg aaatttctgc
    13681 agcgcgtaag gaagtaccgg gtcgtcgcgg ttatcccggc tatctttata cggacttgtc
    13741 cacaatttac gagcgcgccg gccgaatcaa aggcaaaaac ggatccatta cgcagctgcc
    13801 cgtccttacc atgcctgaag acgataaaac acatccgatc ccggatctta ccggatatat
    13861 cactgaaggg cagataatca tgagcaggtc gctccatgcc caaggcattt atccgccggt
    13921 agatgtcctg ccctcactct cccgcctgaa agacaagggt atcggagcag gtaagacgcg
    13981 tgcagatcat gctaacgtga tgaatcagtt gtattccgcc tatgcgcggg gcaaaaatgc
    14041 taaagaactt gccgttgtgc ttggcgagtc tgctttgagt gaaatagatc ttctctatgt
    14101 gaaattcgct gatgcttttg aaaaccgttt tatccgccag ggagaagatg aaaacagaag
    14161 tatagaagat agtctggtcc tgggttggga acttatggct atcctgccta aatcagaatt
    14221 aaaacgcgtt cgcgacgagt ttattgaaaa atattaccct gaaaagggct gaagcctgtc
    14281 gcttaaagag taagggaaca taggtatgcg ccttgcagtc aatccgacac gcatggaaat
    14341 gcttcgcctg aggaatcgcc tgagcgtggc acagcgtggg cacaaacttt taaaagacaa
    14401 actcgatggt ctgatgaaag actttggaga atacgctcag aagtataagg agtgccgtct
    14461 ggcagtggat gcagagctgc ctgtcgtatt gaaacttttt gttttggctg aggccacttc
    14521 ttcccgactg attgttgaag atgcccttga acttacccgt caggaactcg atattacttc
    14581 aatacccacc cgtatcatga gtgtggtaat cccccgtctt gaattcagtt ttggaaaaag
    14641 tctgggcggt tattctctga ttcacacttc tgctgaactc gataaagcca tatccgggct
    14701 gaaggttttc atggaaaaac tactgactat ggctgaactt gaagagacag tcagacgtct
    14761 tgcccaggaa attgaaaaga cacgccgtcg tgtgaatgct cttgaacata ctgttattcc
    14821 ccgtttgctg gagaccatca agtttattaa aaataaactg gacgaaatgg agcgttccac
    14881 aaccagccgc ctgatgaaaa taaaagaaca gcgcctggca caggaaggca aataagcgct
    14941 gccttctttg gctacggtct ttcttattca ggcacaatgc ttctcttcct tcaatggcat
    15001 aaggaaaggt gtacctatgg ctgcagattt aaggttagat tcatttgatg ctgatgaaag
    15061 aaaagcggcg cttctggaag tgtacagatc tgccctgtca actgattcac tccccgcttt
    15121 cggtcgccat ttcaatctcc attgccattc attcttttct tttaacggat acggtttttc
    15181 ccctacacac ctcgcctggc aagggcgcct tttaggactt tgtgccatgg cacaggttga
    15241 cttcgatgtt ttagatggag tcgacgaatt tctgagcgcg tgccgttttc tggatattaa
    15301 agcggcggcc ggcatggaaa gcagagtttt tgttcctgaa ttttctgagg aagaaatcaa
    15361 ctctccggga gagcccggag ttgcttatca tatcggatta ggtttcgctt cttctaaggt
    15421 taaagaaacg gcgcttttgc attcatttaa agacaaggcg caatcccgga cacgtgttgt
    15481 cgtgcaaaag gtgaatacac ttctgcatga aattgctctg gattatgacc aggacgtttt
    15541 gtcactgact ccggcaggaa atgccaccga acggcatgtt tgtgccgcct acgaccgaaa
    15601 agcgcgaaat tactttactg agccatcccg tcttatctct tggtggtcag aaaaacttga
    15661 tctggctaag gagaaaattg aagaggccct ctcctctcca ccggactttc agggaataat
    15721 ccgtgctaaa acgatgaagc aaggcggggt tggctatatc gcatcaaaag gggaagattt
    15781 tccgacactg agagttgtca atcagttttt tttggataat ggtgcgattc ctgtgcttgc
    15841 ttttcttgat ggcacttctt ccggtgagaa acgcatggac gatctgcttg acattatgat
    15901 agattcaggg gtgggcgcag tcaatattat tcctgacagg aactggaata taaaagaccc
    15961 tgatgtgcga gctctcaaaa taagcaaact ggatgaattt atcggcatgg cacggaagcg
    16021 gcatctgccg atcattgtgg gcacggaaat gaatgcccac gggcaggcgc ttgtcgatgc
    16081 tttagacgct ccggcgttgg cgccctattt caaagatttt gaagagggca tgttttttat
    16141 ctatggccat actgttctcg cgtccatggc agagcttggt cattcaagcg catggtctca
    16201 ggcttcattt tcaacggcag cagagaggaa tgctttttat atacaggtag gtcgtttggc
    16261 ttctcctgca attgaaggca acaccctggg aatttcgtca tccgatacgc ctgaaattat
    16321 cctgaaaaaa ttcagccgtg aataattcgg acttcttctt ttaattcaat cccgaaacgc
    16381 ttcagcacct cttcctttgc caaacagatc aggccgtaga catcatcaaa agaagcgttg
    16441 tcctgattta caataaagtt ggcgtgtttt tcactgatta ccgccccacc ccgccgcgtt
    16501 cccttcagcc cgcacagttc aatgaggcgc ccggcgtgat ccccttcagg atttttaaaa
    16561 acacttccgc agcagcgtcc ctgaggttgg gtttcttttc gtcttttaag ataatgctca
    16621 taaacagcca gctggtcttt ctcgtcaacg gtaaaagaaa acacaccttc aagaataaca
    16681 gtattcgtgg tgcaaaagcg ctgacctctg tagcaataat cgtcgctgct catctgccac
    16741 tgcgtaagcc ccgatgctga aagaagctgc acagactccg taaactggca gatagtacca
    16801 ttgacagttc cggcattcat gaaaagagca ccgcccacag aaccgggaat tcctgtcagt
    16861 gcgcctgtgc ctgtgtaatt gttcgggagc agtacctggg tgacaagaga tgccagtatt
    16921 gtcccggcac ctactttgaa tcgattgcat cccagaggcg tactataatc caaacctccg
    16981 gtgaataaga ctgctccgtc atagccttta tctgaaacaa gaacgttggt tccgcctccc
    17041 agaataagga aaggcactcc ttttttctga atccattggt acccctcctg aatttcttcc
    17101 agacttcttg gaaacagtgc ccaccgcatg gtcccgccaa tattataaaa acagtgtggt
    17161 gcaagtgaaa gattttcttt tataaaagca ggcggggaca acttcattta aaatctctat
    17221 ggtttataag gaggcagttc aggaattaag ctggcttcgt ttgagtgttg agagtatttt
    17281 ggttccgcac cgctcccagc tgcaatcgtg tatcaggttt tgtgcggttt gtttgcgctt
    17341 tcgcaagtcg ctcgcttttt catttttggc gcgtcggaga atattcaaaa gagaggtaat
    17401 attgtccgct tcacaataaa aaggcaccga ccctgccact tcattaattg ctccggaacg
    17461 tgttgtaatc agcaaggctc cggaagacaa agcctgcagc acaggtattc cgcttccatc
    17521 tccatgagca aggtaaaagc acatttcggc gttctgcatt aaaccggcca cttctttatc
    17581 agggcaattt tcaatacgaa ccacgggcag aggccagttg gaaggttctt ccgggtgcac
    17641 aggccccagc acaacaagat tttgcgggaa gagggaagtg tttttttgga ttgcgttaaa
    17701 aaaagttggc aaagcgggaa atgtatagcg attcaaaata aaaagtccgt aagggggatc
    17761 aatcaacgaa gggcttggct cagaaaagat tttatcgacc ccagctttgg caacaattgc
    17821 tttttctagc cccatttcca atctggacaa acaactgcga tggacatatt cactgggaca
    17881 taagatggat tgagcagaca tgcaggcacg ttttattttc gaactaaggg gcggtggcgg
    17941 ggtgttgctg actgatctgg tttcgccgtg gaacagcatg tctgcagtga aaagaatctg
    18001 gggaatactc agtcgcaata agggagtgct gatatgagta agtaaaacgt ccagcccttc
    18061 gtcctgcaca actttctcca gaagccgtga ggaagactta aagagggaca gcgacgacct
    18121 gaccggctct atagctgtag ttctgacaaa tgcgcagggc gaagggttgc cgccttcatc
    18181 aaaccaaagc aactccatat ctgaatacgc tttttgcgca tgaacagcgc caagagcacg
    18241 acggaaatac tctttttcaa gtccgtcgta aaaaagagaa gaagccgatt tgcctctttc
    18301 tccaaaaggc atataaatac caactttcat ggagcgattt tacgttgcct tctttgactt
    18361 gttaataata gttaaaaaga gattgcacgc atcaaacaga gcatcggtct gagcgttatt
    18421 ttccggttgc ccattcaata aggatttgtg caacttcggg tcgtgtaaat tcaacaggcg
    18481 gtgcttctcc attagccagc aattcacgaa cttttgtacc acttaaaaac acatgttcct
    18541 cgctgctgcc tggacaggtc tttttggttg ccatatcgtt ggtgcgtttt gaaaagaaag
    18601 catgttcaaa aaataatggt gtaatcccca aggctgccgg atcgatttca tcaaagatat
    18661 aatgggcatc ataactgccg taataattgc cgacacccgc atgatcgcgc ccgacgataa
    18721 aatgggtgca tccgtaattc tttcgaataa tggcatgcat aacggcttca cgcggtccgg
    18781 cgtaacgcat attaaccggc attatagaaa gcatgacgtg ttctttggga taatatttgt
    18841 cgagaagcac ttcatagcat ttcatccgaa tatctccggg aatatcatcg cttttggttg
    18901 tgcccatcag aggatgaatg agaagtccgt cacacatttc cagcgcacat tttgtaaggt
    18961 attcatgggc acggtggatg gggttgcggg tttgaaaagc aactatacgt ttccaaccgc
    19021 gttctttaaa tgcagcccgg gtttctagcg ggctgaggtt atattcctga tgatctaccg
    19081 ggcggctatg ggccactttc aactgccctg ccaaataaac atcacctgtc gaaaatgtat
    19141 aggcaactcc cgggtgggct gtttcggttg ttctatatac tttttcggcc agatattttt
    19201 tgtccaactg gaatttttcc gctatattca tgacggcaat tatctggttg taatcgtctt
    19261 tcagagcgac ttcctgaccc aactcgagag tatctgccac agcggtatca actgaaaaaa
    19321 gaaccggaat ggtccaggga agacctgttt tcaggcacat ggactcaaga acgctcagtg
    19381 tgtcctcttt gcctaaaaaa ccctccagcg gactgaaaat gccttttgca atagcatcca
    19441 catcaaaagc agtataagca tcaatctgga taactggcag gcttgcagca tgccgtttaa
    19501 aggattccag gtcgcccgct tcaataaaac aattttttag ctctccacca tgaggcgcaa
    19561 tagacataaa atgactccct tggtatatga aggatagaat ccgcttcttc gcgtatatta
    19621 ggttcaagta tatcaaaggt gcttcttacg ggacaaaaga ttgcctgc
//
LOCUS       DAOPWB010000054        19523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2821_length_19523_cov_4.852168, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000054 DAOPWB010000000
VERSION     DAOPWB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 19523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19523
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_19523_cov_4.852168"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PKN92_06735"
     CDS             complement(<1..1346)
                     /locus_tag="PKN92_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNZ48525.1"
                     /translation="MLIPSLDEFRRLAHQNAIIPVYKEILADLETPVTAYLKLSRYTP
                     CSFLLESVEKAENIGQYSFIGANPSTVFRSKGEAGVLIENGVEHPFTSASPLDRLKDY
                     MAQYKPVSVPGLPAFHGGAVGYVSYDEVRHFEKLPEENPDTLGLPDLYFVITDTLVIF
                     DHANNKIKIVSNAHVQDNPDAVYNEASRKISEIEERLRGPLVVSTERLHSAPTEPLIS
                     SNFTRESFCEAVEKAREYIRKGEIFQVVLSQRFKREVGISPTNLYRALRCINPSPYML
                     LLQFPDFALVGSSPEVMTQVKHGRAMVRPIAGTRRRGITPEEDLALEAELLADEKEIA
                     EHVMLVDLGRNDLGRISEPGTVEPVPHKYMVIERYSHVMHIVSEVTGTLKKDCSALDA
                     LIATFPTGTVSGAPKIRALEIIEELEPEQRRPYAGACGSISYSGDMDTCILIRTMVV"
     gene            complement(1501..2277)
                     /gene="hisF"
                     /locus_tag="PKN92_06740"
     CDS             complement(1501..2277)
                     /gene="hisF"
                     /locus_tag="PKN92_06740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNZ48526.1"
                     /translation="MHTKRIIPCLDVVDGRVVKGVKFLGLRDAGDPVEIAHRYDEEGA
                     DELVFLDIRASTDNRATLVDVVERTAAGLTIPLCVGGGIRTLDDIQRILDAGASKVSI
                     NTPAIERPDLINEAADRFGSSCLVVAIDAIEKDETSGGGYIVKSHGGRDGGRTTSLDP
                     VTWAQEAESRGAGEILLTSMSQDGVKAGYDIALTRLVADAVKVPVVASGGAGSLEHLR
                     AALQEGGADAALAASIFHFREVTIREAKQYLHSKGISVRL"
     gene            complement(2324..3436)
                     /locus_tag="PKN92_06745"
     CDS             complement(2324..3436)
                     /locus_tag="PKN92_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48527.1"
                     /translation="MKLLILKLIALLLLLGGALMLGMALNLDQAWSVYNQCLGYWPAV
                     ENGTPYFGIPGIFLGAFMTLLGLYAFLPRIPSAKNKYITYAGDQGTVMLELAPMRKSL
                     LKILGKMDEIAKIKIQLRPDRRRQHVCVTANVILNSRKDFSKEADAKTIARCLSVACR
                     EVLGFEGISSVVVNVRGIRLNPAETEKKIRKHLSELDAQKEAVASPPANGSDATAAEK
                     ESEIKEQPEASSDTTTPLKETPAISESPAPVAAPAPAEEQNEASSEQEEPPVADVSSD
                     TDNAPCCCASAAEDDTPEEADNADTLPESPAPEEAAENEAVGTCAADDTVCEESCPET
                     LPPLASEEEDIPDALPEAVNSDDSEEEEKNSGTSWQ"
     gene            3595..4494
                     /locus_tag="PKN92_06750"
     CDS             3595..4494
                     /locus_tag="PKN92_06750"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNZ48528.1"
                     /translation="MTQQKGLSWKIWLLAARPRTLPAAAAPVILGTVFAWKEDSFHFL
                     SALAALAGALLIQIGANYANDYYDCLKGADEGERLGPVRATQAGWVTPGQMLFATACV
                     LVSAAIPGLYLVWRGGIPILVIGLLSLLFAVLYTGGPWPLGYLGIADLFVLFFFGPVA
                     TGGTFYVQAHFLSIPAVLIGFSTGLLSVAILSVNNLRDYAQDLKSGKKTPAVRFGKTF
                     AKWEYAVSLYLALAGVPLLLVWHDPTVVLCLAVLLLCVPGHCLIKRVWVQEGAILNQR
                     LADTGKLLLAFSIFFSALWIWSF"
     gene            4660..5589
                     /locus_tag="PKN92_06755"
     CDS             4660..5589
                     /locus_tag="PKN92_06755"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNZ48529.1"
                     /translation="MAAMHPGPVAYFNGKFVPVEEAKISIMTHAFNYGTGLFEGIRGY
                     YNVEEDNILIFRLPEHVDRMMRNAKVLCMDIPEDGAAIEEICLELVKKSGFKEGVYIR
                     PIIYKSEQSLGPTVLGVASSLCCYIIKLGDYCDTEKGLDVAVSSWRRLSDNAIPTRLK
                     STGSYINSSLSASEAKSAGFHEAIFLREDGTVAEGSAMNLFMVHGGKLITPPVNADIL
                     VGITRNTVIELAREALGIEVVERPIARTELYICDELFFCGTGAQVAPVRSVDRRLIGK
                     GTTGVITKKLQDLYFNVVQGKVPEYRKWCREVY"
     gene            5604..5993
                     /locus_tag="PKN92_06760"
     CDS             5604..5993
                     /locus_tag="PKN92_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48530.1"
                     /translation="MASFILRCRMVFFSWRFLLNLLLVLVITSWIPLLAQTYTLKDSN
                     GVVLQEQTVKVRAWTSWHKMMKPGAPDFKSHLYAVSTHFGVCLLISFSVWFVTRRQLQ
                     KKMLRTEHTGGDPDSFNTPPEKGEDHD"
     gene            5986..6648
                     /locus_tag="PKN92_06765"
     CDS             5986..6648
                     /locus_tag="PKN92_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNZ48531.1"
                     /translation="MTEMLLRNWICEIGRRLHQRDMIAAADGNISVRFGDDQFLCTPS
                     GVSKGFLRPDDIVIADSCGEKVAGEGKVTSEFFTHLAAYQNRPETQAVIHAHPTYATV
                     LTIMGVNTTAPILPEVVMNMVALPVAPYATPGSREGADAITALIKEYDALLLERHGAI
                     TVGADLQEAYMRMERLEHAAKTLYLCSLLGKPPVIPPDELAKLMAVAVPSCTPPPPYP
                     FP"
     gene            6978..7760
                     /gene="thyX"
                     /locus_tag="PKN92_06770"
     CDS             6978..7760
                     /gene="thyX"
                     /locus_tag="PKN92_06770"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019158914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNZ48532.1"
                     /translation="MKTVVPKVFLVGETRIVPEGLEQFLEHVGAPEWKSDAPSDSELL
                     CEVMGRLCYRSFSPGLNPNVTRVREGNKPYLSNILQVGHGSVLEHASLNFIFADVSRV
                     VTHELVRHRAGVAISQESLRFVRLDNLSAYVPVHIRENEKGMEVFVRTIEHLEELQRE
                     LAEIYQINEEKQFDVKKKLTSAFRRIAPIGLATTIGWSCNFRTVRHVIEMRTDPHAEE
                     EIRFLFAEVYRLVCERYPNLFSDYEVEMVDGLPWVKTGNRKV"
     gene            7844..9886
                     /locus_tag="PKN92_06775"
     CDS             7844..9886
                     /locus_tag="PKN92_06775"
                     /inference="COORDINATES: protein motif:HMM:NF037695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused protease/ribonucleoside-triphosphate
                     reductase"
                     /protein_id="HNZ48533.1"
                     /translation="MFAVRERFALDARFLAQFKDRQPDWGPLGYITYKRTYARVVPDD
                     PNGRTEEFWETLQRVVEGCYTIQWNHCASLKLPWKPEKAQRSAQEMFRLMWDFKFLPP
                     GRGLWMMGSEYIYQRGSAALNNCAFVSTEEIDTDFAEPFCFLMDMSMLGTGVAFDTKG
                     AGKVTIRSPQTGEYTHVVADSKEGWADLVRTILNAYVGRGKRPSEIDYSKIRPQGSQI
                     KTFGGIAPGPDPLKECISNIDKILTPRDGHPISSTDITDLMNVIGKCVVSGGVRRTAE
                     LALGSPDDEEYLQLKDPKRCQEQLMAWRWASNNSVIAREGMDYMKAGTQTAFNGEPGY
                     FWLENARAYGRLKDGPTYADAKVAGTNPCAEQSLESYEICNLVETFPSRHESLEEYKR
                     TLKFAYLYAKTVTLIPTHNERTNAIMLRNRRIGLSQSGIVESFTRHGRREHFRWCDEG
                     YQYICKLDRIYSQWLCVPESIKRTSIKPSGTVSLMPGVTPGIHYPHSQYYWRTIRLDK
                     TSALVEPLRKAGYRIEESVYGDNTLVVYFPVKEKFFLRSKNDVSVWEQVENVAQIQYY
                     WADNQVSATITFKPEEALDIPYILELYETRLKSISFLPLVDHNYPQAPYQAISKEAFD
                     LAVSKLSVPDFSTMNTDETQDLYCDGDKCAAPVMTPIHEQQEVDFDGTEEEEAVIE"
     gene            10318..11010
                     /gene="aqpZ"
                     /locus_tag="PKN92_06780"
     CDS             10318..11010
                     /gene="aqpZ"
                     /locus_tag="PKN92_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNZ48534.1"
                     /translation="MSLKSVSAEFIGTFWLVLGGCGSAVLAAAFPEVGIGLLGVALAF
                     GLTVLTMAFAIGHISGCHLNPAVSVGLAASGRFPVAKLPAYIIAQVLGAVAAAGVLYF
                     IASGKAGFDLGSGLASNGFAEHSPGGYSLGAGLVTEIVMTFMFLMIILGATDKRAPQG
                     FAPIAIGLGLTLIHLISIPVTNTSVNPARSTGPALFVGGWAVKQLWLFWVAPLIGAAF
                     AGILYKWFEEEN"
     gene            complement(11403..11477)
                     /locus_tag="PKN92_06785"
     tRNA            complement(11403..11477)
                     /locus_tag="PKN92_06785"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11442..11444),aa:Glu,seq:ctc)
     gene            complement(11519..11594)
                     /locus_tag="PKN92_06790"
     tRNA            complement(11519..11594)
                     /locus_tag="PKN92_06790"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11559..11561),aa:Gln,seq:ctg)
     gene            complement(11735..12733)
                     /locus_tag="PKN92_06795"
     CDS             complement(11735..12733)
                     /locus_tag="PKN92_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNZ48535.1"
                     /translation="MPPKHNPFASPSEKMMGLLSIFLFTERPHSLTQLAAILQCSKQS
                     VLRIFEQIQKSDWVTVDSWITKKQRWYQTRIVKKPSNVVLDSTAIERLLLCKDMVWHL
                     LPDAYRKEITAALKNAAKQTYAEPQIVPDSFTQVKPKGYIDYSSREQFISDLIRAIRE
                     NRICEISYKSPSRKAAKSYQVAGYQMVVFREGLYLRCRPVAELEAEKPEDIILSVHRM
                     HSVSLTDQFFEAITPQDSAEDIAGTFGLRQGEAFRATVKVAATAAMYVQERVWSGDQK
                     IKNHADGSLTLHFSTTSEEETLAWVLSFGGAMELVEPVSLRKKIAEAAKAMATAHK"
     gene            13048..13968
                     /locus_tag="PKN92_06800"
     CDS             13048..13968
                     /locus_tag="PKN92_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48536.1"
                     /translation="MKHLTGFALLTTALLLTMLISGYAEDRPLEIYVLMKTDPESPEQ
                     MVKVYVDYVEPRDPETPWCHDPDISAIQDKRIVALWEDIIISYRRNHPTGATVRGLDD
                     APEFIAEPNTPENREILIDALSLALSADPVDWNYVLTVLVLMQKGMAPDLRITQLADR
                     LFTARRNEEMSYDHGECIRTMYRVLKRQKTKEAAELLYRACSRDYWGEDPMLTLSFSE
                     KWSEHSIITVRKFALSCLSEDMPPGLSIPLLEELASVYPDSYVPPDIIVQPADGLRSE
                     RRIIIDFSRSDIEEALEKAYKKQAAEESGN"
     gene            complement(14708..15613)
                     /locus_tag="PKN92_06805"
     CDS             complement(14708..15613)
                     /locus_tag="PKN92_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48537.1"
                     /translation="MKKLVTVCLAAAFIFAAGAYAEEACPDSLLEQVLLLQFAEKAGI
                     DDYDLIEVLEGYRAYRSAMDAYTKQRAAKEAALKAAIANDDSDNVVSGLTRELMALDL
                     SILRLKQSTMDEAADIMSAAEVAELYLMVSNFDAVKAELAGKLAASQCTAAPCPIAAA
                     CPAAADCPIAAACPDAKPASAAAPAAAPSGPEAILARATEFLNKLFAGNLDAALEGVS
                     EDFQNSEYADKAELKDFLESAIAMGFLEDASIDLEDTEVTIEGDTATVYPLDISGNFG
                     SGTLEFVLKNENGKWVLVGFDAFGI"
     gene            complement(15715..16926)
                     /locus_tag="PKN92_06810"
     CDS             complement(15715..16926)
                     /locus_tag="PKN92_06810"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNZ48538.1"
                     /translation="MSSVKKIVLAYSGGLDTSIILKWLQEAYNAEVIAYIGDVGQGEE
                     TEPARVKALNTGASQAIVKDLREEFVRDFVFPTMRANAVYEGVYLLGTSVARPIIAKA
                     QIDVLRETGADAVAHGATGKGNDQVRFELTCFALEPNVKIIAPWRDPKWTLDSREKMI
                     AYAEKHNIPVPVSKAKPYSSDRNLLHISFEGGILEDPWNEPPEDMFQLSVSPLKAPDT
                     PHYVEIDFEEGTPVAVDGKRMAPVELLETLNALGGAHGIGRVDLVENRFVGMKSRGVY
                     ETPGGTILYAAHRAVETLTMDREVMLQRDILSPKIAQLIYNGFWFSPEFEALMAFVNK
                     SQETVTGTARLRLYKGNCDVVGRRAEKTLYDPKICTFEEDEGAYDQTDATGFIRLNAL
                     RLRARKNAGLF"
     gene            complement(16966..17880)
                     /gene="argF"
                     /locus_tag="PKN92_06815"
     CDS             complement(16966..17880)
                     /gene="argF"
                     /locus_tag="PKN92_06815"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNZ48539.1"
                     /translation="MVKDFISLADFTADELMHFLNLADELKAQKKAGIPHPLLNGKTL
                     ALIFEKPSLRTRLTFQVGMYQLGGTTQLIDTRLGERESVADVARNLERWVDGIMARTF
                     AHKHVIELAEKASIPVINALTDKLHPCQILADVQTLREHKGKDLSSLKVAFVGDGNNV
                     FHSWANFVSRLPLNLTLVCPEGYEGDPEIIERVRKETRAEFVITQDPEAGLKDADAVY
                     TDVWASMGQEDEAAERAKIFAPYQVNSTLMGWAKKDALFMHCLPAHRGAEVTSEIIDG
                     PNSVCFDQAENRLHAQKAVLATLMGGVS"
     gene            complement(17874..19076)
                     /locus_tag="PKN92_06820"
     CDS             complement(17874..19076)
                     /locus_tag="PKN92_06820"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNZ48540.1"
                     /translation="MTTLNTQEIKTLNTEHILNTYGDRRLALVRGEGARVWDAEGREY
                     LDFFAGIAVCNLGHCHPKVTEAICTQAKKLVHVSNLYYIEPQVKLAEKLSRHCFADKW
                     FFCNGGAEANEAAIKLARRYWTMIGANKPEIVVAEHSFHGRTLGALSATGQPKYHYGF
                     DPLVPGFHYVPYDDLKALDAAITPQVGLVILEPIQGEGGVRVPSPGYLPGVRRLCSDK
                     NVLLAFDEVQTGLGRTGTLFAYAHENVIPDIITLAKGLGNGVPIGAMGCTEKLSEGFS
                     PGAHACTFGGNPLSAAAALATFEVLTEPGLVEKSAEMGAYFIRQLVDLSLEHKSILEV
                     RGRGLMIGIEFDREVAPLVAAMHDAGILCGIAGPRVLRFLPPLIIDSTAVDCVIAALE
                     ASLEALQW"
     gene            complement(19100..19387)
                     /locus_tag="PKN92_06825"
     CDS             complement(19100..19387)
                     /locus_tag="PKN92_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ48541.1"
                     /translation="MPTYSYQCKRCSAVQDVFHGISQSPRVKCAECGGACTRLLGSGA
                     GIIFKGTGFYETDYKNKSNKKSSKATTKSSSESKTDQSTSGTVSSSDKKAS"
BASE COUNT         4830 a         4737 c         4984 g         4972 t
ORIGIN      
        1 acaaccatgg ttctgatcag aatgcaggtg tccatatctc ctgaatagga aatactgccg
       61 caggctcctg cataagggcg acgctgctcc ggctccagtt cttctatgat ctccagggcg
      121 cgaattttgg gcgctccgct tacggtgcct gtggggaaag tggcaataag tgcatccagt
      181 gccgaacagt cttttttaag cgttcccgtt acctcactca caatatgcat cacgtgtgag
      241 taccgctcaa tgaccatata tttgtgcggt accggctcca ctgtgcccgg ctcgctgatc
      301 ctgcccagat catttcttcc cagatcgaca agcataacat gttcagcgat ttctttttca
      361 tcagcgagca gctccgcttc aagagccaga tcctcttcag gcgtgatgcc gcggcgtcgc
      421 gtgcctgcga tgggacgcac catcgcacgg ccatgtttga cctgcgtcat tacttccggg
      481 ctcgatccga ccagagcaaa gtcggggaat tgaagcagca gcatatacgg ggaagggtta
      541 atgcaacgca gggcacggta aagattggta gggctgatac ccacttctct tttaaaccgc
      601 tgtgacagaa caacctgaaa tatttcgcct ttccggatgt attccctggc tttttcaact
      661 gcttcacaga atgattcccg ggtgaagttg gaagaaatca gaggctccgt aggtgcgctg
      721 tgcagacgtt ccgttgaaac gacaagggga ccgcgaagac gctcttcaat ttctgagatt
      781 ttgcgggaag cctcgttgta cactgcatcg ggattatcct gtacatgggc gttggaaacg
      841 atttttattt tgttgtttgc gtggtcgaat atgacaagag tatcggtaat gacgaaatag
      901 agatcgggga gcccgagtgt atcgggattt tcttccggca gtttttcaaa gtgacgcact
      961 tcgtcataag aaacgtagcc gactgcaccg ccgtgaaacg cagggagccc gggaacagaa
     1021 acgggcttat attgagccat gtagtctttc agacgatcca gagggcttgc cgatgtaaag
     1081 gggtgctcca caccgttttc aatcaataca cccgcttctc cttttgaccg gaaaacagtg
     1141 gagggattgg caccaataaa tgaatattgt ccgatgttct ccgccttctc cacactttcc
     1201 agcagaaaag agcagggcgt atagcgggac aatttcaaat aagccgttac aggtgtttca
     1261 agatccgcca gaatttcttt gtagacgggg ataatggcgt tttgatgggc gagccgccgg
     1321 aactcatcaa gagaggggat taacataaaa aggattcctt ttagttaaag agaataaaca
     1381 accgtggcgc tggatatctg ttccagacac gcatttcaga agaaggcgga gcgtcgccac
     1441 cagcgtacgt gttgttcgag ccctttcata aaagtaagtc cttccagttg catgtttaag
     1501 ttaaaggcgc acagaaatac ctttgctgtg aagatattgt ttggcttccc tgatggtgac
     1561 ttcacggaag tggaaaatgg atgctgccag ggcggcatcg gcaccgcctt cctgaagagc
     1621 agcccgcaga tgttccaaac tgcctgcgcc gccgcttgcg acgacgggca ccttaacagc
     1681 atcggcaacg agtcgtgtaa gagcgatgtc atagccggct tttacaccgt cctgtgacat
     1741 ggatgtcagg agaatttcgc cggcaccccg ggattctgct tcctgcgccc aggtgaccgg
     1801 atccagggat gtcgtacgtc cgccatcccg gccgccatgg ctttttacaa tatagccacc
     1861 gcccgatgtc tcgtctttct ctattgcatc tattgccaca accagacagc ttgaaccgaa
     1921 acggtctgcc gcctcgttaa tcaagtccgg ccgttcaatc gccggtgtat tgatcgatac
     1981 tttactggca cctgcatcga gaatccgctg aatatcgtca agcgtgcgga tcccgccgcc
     2041 gacacataac ggaatggtta gcccggcggc tgttcgttcc acgacgtcta cgagtgtagc
     2101 acgattgtct gtagaagcgc ggatatccag aaagacgagt tcatcggcgc cttcttcgtc
     2161 gtaacggtgt gcaatttcaa caggatctcc ggcgtctctc agtccaagga acttgacacc
     2221 ttttacgaca cgcccgtcca ccacatccag acagggaatg attcgtttcg tatgcataaa
     2281 accttctttt tccaggaaaa aatccaaaaa taaaagcaaa cgcttattgc caggatgtgc
     2341 ctgagttctt ttcttcctct tcggaatcat cactgttcac tgcttcaggc agggcatcag
     2401 gaatatcttc ctcttcggaa gccagcggag gcagtgtttc cgggcatgat tcttcacaaa
     2461 ctgtatcatc ggctgcacag gtgccgacag cttcattttc tgcagcctct tccggcgcag
     2521 ggctttccgg cagggtgtcc gcattatcag cttcttccgg tgtgtcatcc tcagcagctg
     2581 atgcacagca gcagggagca ttgtccgtat cggagctaac gtcagcaacg ggcggttctt
     2641 cctgttcgga tgatgcttcg ttttgttctt cagcaggagc aggtgccgcc acaggggcgg
     2701 gactctcaga aattgccggg gtttctttca gaggcgtggt tgtgtctgaa gatgcctctg
     2761 gctgttcttt aatctcggat tctttttctg cagcggtggc atccgatcca ttagcaggcg
     2821 gcgatgctac ggcttctttc tgcgcgtcca gctcactgag gtgttttcga attttttttt
     2881 cggtttcagc aggattaagc ctgataccgc gaacattcac cactacagag ctgattcctt
     2941 cgaaaccgag cacttccctg caggcaacag aaagacaccg ggcgatcgtt ttcgcatccg
     3001 cttctttgct gaagtctttg cggctgttca gaatgacatt ggcggtaaca caaacatgct
     3061 gcctgcgacg atccggacgc agctggatct tgatctttgc aatttcgtcc atcttcccca
     3121 gaatttttaa aagggacttg cgcatgggtg ccaactcaag catgacagta ccctgatcac
     3181 ccgcataggt gatgtacttg tttttggcgg aaggaatgcg cggtaaaaag gcataaagac
     3241 caagcagtgt catgaaagca cccagaaaaa taccggggat tccgaaatag ggggtaccat
     3301 tttcaacggc aggccagtaa cccaggcatt ggttgtagac actccacgcc tgatccagat
     3361 taagcgccat acccagcatc agggcgcctc cgagaagaag aagcagggcg atcagtttta
     3421 aaatcaaaag tttcatagcg ctatccttta tatctgataa atgaaaataa cgaacgggaa
     3481 aacaaataag gataatcgtt tatttactat tggaaataat cgtatcataa atggcatatg
     3541 cactgcgatt tgtcacatag cgaatgaccg ataaaggaat tgactgccgt gcctatgaca
     3601 cagcaaaaag gactcagttg gaaaatatgg cttttagcag cacgtccccg cactctgccc
     3661 gctgccgccg ctccggtgat actcgggact gtttttgcct ggaaggaaga ctcttttcat
     3721 tttctttccg ctttggcggc tctggcgggt gctctgctga ttcaaatcgg cgcaaattac
     3781 gccaacgatt attatgactg cctgaaagga gcagatgagg gagaacggct ggggcccgta
     3841 cgggcgaccc aggccggttg ggtaacaccg ggacaaatgc tttttgcaac tgcttgcgtt
     3901 ctggtttcag ccgcgatacc gggattgtac ctcgtatggc ggggtgggat tcctattctg
     3961 gtgataggtc ttctctccct tcttttcgct gtgctttaca ctggagggcc ctggcctttg
     4021 gggtatctgg gtatagccga tttatttgtt ctgttttttt tcggtcctgt tgcaacaggg
     4081 ggaactttct atgttcaggc gcactttctt tccataccgg cagttctgat aggcttttca
     4141 accggtttgc tgtctgttgc aatcctcagc gtcaacaacc tgagagacta cgcacaggat
     4201 ttaaaaagcg gcaagaaaac gccggcggta cgctttggaa agacgtttgc aaaatgggaa
     4261 tatgccgtgt ctttatatct ggcactggcg ggtgtgccgc tgctgctggt atggcatgat
     4321 ccgactgttg tgctctgtct ggcggttctc cttttatgtg ttccgggaca ctgcctcatc
     4381 aagcgggtct gggtgcagga aggagccatt ctgaaccagc gcctggcgga caccggaaaa
     4441 ctcctgcttg cgttcagtat cttcttttca gcactctgga tatggtcgtt ttaaggcgat
     4501 ttcaggtgca ttgtgctgag gttttgccgg cggacagaaa ggcgccgctc tcccgggtaa
     4561 tttgcattac acgcaagaaa agcgcatact aaaggccttc agatactgca acccctcccc
     4621 attaccaagg gaatagtttc aaatagtgga gatatcatta tggctgctat gcatcctggt
     4681 cccgttgctt attttaacgg aaaatttgtg cctgttgaag aagcgaaaat cagcattatg
     4741 acccatgctt tcaattacgg aacaggtctt tttgaaggga tccgcgggta ttacaacgtt
     4801 gaagaggaca atatactgat ctttcggctc ccggagcatg ttgaccgtat gatgcgtaat
     4861 gcaaaagtcc tgtgcatgga cattccggaa gacggtgccg ccatcgaaga aatctgtctt
     4921 gaactggtga agaaaagcgg cttcaaagag ggcgtatata ttcgtcccat tatttacaaa
     4981 tccgaacaat ctctcggacc gacagtgctc ggcgtcgctt ccagcctctg ctgctacatc
     5041 ataaaattgg gtgactactg cgacacagaa aaaggccttg atgtcgctgt gtcctcctgg
     5101 aggcgtttgt cggacaatgc aataccgacc cgcctgaagt ccacaggaag ttacatcaac
     5161 tcctctctct ccgcatcgga ggcaaaatcg gctggcttcc acgaagccat tttcctgcgt
     5221 gaggacggaa ccgtcgccga aggcagtgcc atgaatttgt ttatggtgca tggaggaaaa
     5281 ctaattacgc ctcccgtgaa tgcggatatc cttgtgggta tcaccagaaa tacggtgatc
     5341 gaacttgccc gggaagcatt gggcattgag gtcgttgaac ggcctattgc aagaacagaa
     5401 ctgtatattt gcgatgaact ctttttctgc ggaaccgggg ctcaggtggc gcctgtaaga
     5461 tccgtggaca gacgtctcat aggtaaaggc accaccggcg ttattacaaa aaaactgcag
     5521 gatttgtatt ttaacgtggt gcagggaaaa gtgcctgaat accggaagtg gtgccgggaa
     5581 gtctattgag agcaggagat atcatggcct cttttatcct tcgctgcagg atggttttct
     5641 tttcgtggcg tttccttctg aatctgttgc tggtactggt cattacatcc tggattcctt
     5701 tgctggcaca gacctatacg ctcaaagatt ctaacggcgt tgtgctgcag gagcagaccg
     5761 taaaagtgag agcctggaca tcctggcaca agatgatgaa gcccggtgct ccggacttta
     5821 agtcgcatct ctatgcagtt tcaacgcatt tcggtgtgtg cctgctgatt tccttttctg
     5881 tttggtttgt cacccggaga cagttacaga aaaaaatgct tcggacagaa cacaccggcg
     5941 gcgatccgga ctctttcaat acaccacctg aaaagggtga agaccatgac tgaaatgctt
     6001 ctgcgaaatt ggatttgtga gataggaaga cggctgcatc aacgggatat gattgctgct
     6061 gcggatggga acatcagtgt gcgctttggt gacgaccagt tcttatgcac acccagcgga
     6121 gtttccaaag gctttctgag accggatgac atcgtaattg ctgatagttg cggtgaaaaa
     6181 gtggcagggg aggggaaggt aacctccgaa ttttttacac acctcgccgc ttatcagaat
     6241 cgtcctgaaa cacaggctgt cattcatgcg caccccactt atgcaaccgt tctgaccatt
     6301 atgggtgtca atactacggc accgattctg cccgaagtgg tgatgaacat ggtcgcactg
     6361 cctgttgctc cttatgccac tcccggcagc agagaagggg cggacgccat aaccgcactc
     6421 ataaaagagt acgatgcact ccttcttgag cgccatgggg cgattacggt gggcgccgat
     6481 ttgcaggaag catatatgcg catggaacgg ctcgagcatg ccgccaagac gctttatctc
     6541 tgcagccttc tgggaaaacc tcctgtaata ccgcccgatg aactggccaa actcatggcg
     6601 gtcgctgttc cttcctgcac gcctcctccg ccatacccct tcccctaagc cggttcaggc
     6661 gaatcgcatt tctgttcttg aagacttcga cattacgtct tttttataag aataatgcga
     6721 atgtcagagg tcttttggca aagatgcgca tcagcacggt atgcaagcgc aaaccagtta
     6781 acgcaaaccc ctcgaattat tgaactttta cgcctaaaaa aaacaagctt gatatccgtg
     6841 gaaagataat ttataatcaa catgttgtat ggttttgttt taatacccca aaatcatgcg
     6901 gtagtggaag gaaaaagccg gctgacttcg cggcatcgcg aagggctttt tatccgcatg
     6961 cggtcaagga gcggcacgtg aaaaccgttg ttcccaaagt atttcttgtc ggtgaaacgc
     7021 ggatcgttcc cgagggcttg gagcagtttt tagagcatgt gggggctcca gaatggaaaa
     7081 gtgatgcgcc cagtgactct gaactcctgt gcgaggtgat gggcagactc tgctaccgtt
     7141 ccttcagccc cggtctcaat ccaaatgtga cccgggtgcg cgaaggcaat aagccctatc
     7201 tgtccaacat actgcaagtc ggccacggca gcgttcttga gcatgcatcg cttaatttta
     7261 ttttcgctga tgtgagccgt gtagtgaccc atgaactggt acggcaccgc gccggtgtcg
     7321 ccatctcgca ggaatcgctg cgctttgtgc gtttggacaa cctctccgct tatgtgccgg
     7381 tgcacattcg tgaaaacgaa aagggcatgg aagtttttgt ccgcaccatt gaacatctgg
     7441 aagaactgca gagggaactt gcagagatct accagattaa cgaagaaaaa cagtttgatg
     7501 tcaagaaaaa gctgacttcc gctttccgtc ggattgcgcc cataggactt gccacaacca
     7561 tcggctggtc ctgcaatttc cggacagtgc ggcatgtgat cgaaatgcgt accgatcccc
     7621 atgccgaaga agaaatcaga tttctctttg ctgaagtcta ccgtctcgtt tgcgaacgct
     7681 atccgaacct tttctctgat tatgaagtgg aaatggtgga tggattgccc tgggtgaaaa
     7741 cagggaatcg caaggtctag ggaaaaatat gctatcagat gcgctgctga gagcgactct
     7801 gatgctgtaa aagataaaat caacagttca ggagagccac gccatgtttg ctgtcagaga
     7861 gcggtttgct ctggatgccc gttttctagc tcaattcaaa gatcgccagc ccgactgggg
     7921 ccctctcggg tatatcacct acaagcgcac ctatgcgcgg gtggttcccg atgatccgaa
     7981 cgggcgtaca gaagagttct gggaaaccct tcaacgtgtg gttgaaggct gctacaccat
     8041 ccagtggaat cactgcgcca gtttaaaatt gccctggaaa cccgaaaaag cgcagcgctc
     8101 cgctcaggaa atgttccggc tcatgtggga ttttaaattt ctgccgccgg gcagaggtct
     8161 gtggatgatg ggttccgaat acatttatca gcgcggaagc gcagccctca acaactgtgc
     8221 ctttgtctcc accgaggaaa tcgatacgga ctttgccgag cctttctgct ttctcatgga
     8281 tatgtccatg ctcggtaccg gggtggcttt cgataccaaa ggggcgggca aagtaaccat
     8341 ccgttcccca cagacaggcg aatataccca tgtagtggca gactccaaag aaggatgggc
     8401 ggatcttgtc cgtacgattc tcaacgccta tgtcgggcga ggcaaacgcc ccagtgagat
     8461 cgactacagc aagatcagac cgcagggctc acagatcaaa acctttggcg gaatcgcccc
     8521 cggacctgat cccctgaaag aatgtatcag caatatcgat aagattctga cgccaagaga
     8581 tggacatccc atctcatcca ccgatatcac agaccttatg aatgttatcg ggaaatgtgt
     8641 cgtttcgggc ggcgtgcgtc gtacggcgga gctggcgctc ggttctccgg atgatgaaga
     8701 atacttgcag ctgaaagatc ccaagcggtg tcaggagcag ctgatggcgt ggcgctgggc
     8761 ttccaacaac agcgtcatcg cccgggaagg catggactac atgaaggccg ggacgcagac
     8821 cgctttcaat ggcgaacccg gttatttctg gcttgagaat gccagagctt acggacgtct
     8881 gaaagacgga cccacttatg ccgacgccaa agtggcaggc acaaatccct gtgccgagca
     8941 gagcctcgaa tcctatgaga tctgcaatct ggtcgaaact tttccctccc gtcatgaatc
     9001 actcgaagaa tataaacgaa cccttaaatt tgcttatctc tatgcaaaaa ccgtgacatt
     9061 gattccgacc cataacgaac gtaccaatgc cattatgctg cgcaaccgcc gtattggttt
     9121 gtcacagtca ggcattgtcg aaagttttac ccgtcatgga cgccgtgagc atttccgctg
     9181 gtgcgatgaa gggtatcagt acatctgcaa actggatcgc atctattctc agtggctttg
     9241 tgtgccggaa agcatcaaac gcaccagtat caaacccagc ggcactgttt cgctgatgcc
     9301 gggcgtgacc cccggaatcc attatcccca cagccaatat tactggcgca cgatccggct
     9361 ggataaaacg agtgccctcg ttgagccatt gcgtaaagcg ggataccgta ttgaagaatc
     9421 agtttatggc gacaatacgc tggtcgttta tttccctgta aaagaaaagt ttttcctgcg
     9481 cagcaaaaac gatgtgagtg tctgggaaca ggttgaaaat gtcgctcaaa ttcaatatta
     9541 ctgggcagat aatcaggtga gtgccacgat cactttcaag ccggaagagg cgctggatat
     9601 tccttatatt ctggaattgt atgaaacccg cctcaaatca atttcttttc tgccgctggt
     9661 cgatcataac tatccgcagg caccgtatca ggctatcagt aaagaagcct ttgatcttgc
     9721 tgtatccaaa ctcagtgtcc ccgacttcag tacaatgaat acggatgaaa cacaggatct
     9781 ctattgtgac ggagataaat gtgctgctcc tgttatgacg cctatacatg aacagcagga
     9841 agtggatttt gacgggaccg aagaggaaga agcggtgata gaataatacc cgaaaagaaa
     9901 attcagtgtc gcagcggagt gaatgctccg ctgctttttt atcaaaaaaa aatagacgat
     9961 ggcccctttt atctttttgc gaagaggact tcagacgcag gcatgcggca aaaagttatg
    10021 gcggccttgc aaaggggaag atttgccaca gagacacctt ttgatcggcg tctgtatgtt
    10081 ttctgcatga aagccaatta tacgaaaaac agtgtttagg aatagggagt aaaaaaataa
    10141 agtgccacac ggattcaaaa atatgtggta tagttactaa ggtatcctta atggatagag
    10201 gtgaacgggc gggatgaacg cactcctaat gagcaagtgt atctatattt tcgaatccgg
    10261 ttttcgattg cagttgtttt ttgttctttg acacctcttt tgaagaaagg aaaaaacatg
    10321 tctcttaaaa gtgtttcggc ggaatttatc ggtaccttct ggctggtact gggtggatgt
    10381 ggaagcgctg ttctggcggc agcatttcct gaagtgggca tcggacttct tggggtcgcc
    10441 ctggcttttg gcttgactgt attaacaatg gcctttgcta tcgggcatat ttctggatgt
    10501 catttaaacc cggcagtatc cgtaggactg gcagcctccg gccgctttcc tgtggcgaaa
    10561 ctgcctgcct atattatcgc gcaagtattg ggcgctgttg ccgccgcagg cgttctctat
    10621 ttcatcgcca gcggaaaggc aggttttgac cttggcagcg gtcttgcatc taatggtttt
    10681 gcagagcatt ctccgggagg ttattctctt ggcgccggtt tggtgacaga aatcgttatg
    10741 accttcatgt ttttaatgat tattctgggc gccacagata aacgtgctcc tcaaggcttt
    10801 gcgcccattg ccatcggtct gggtcttacg cttattcatc tgatcagtat cccggtgact
    10861 aatacttccg tgaaccctgc ccggagtacc ggaccggctc tttttgtcgg cggttgggca
    10921 gtaaagcaac tctggctttt ctgggtcgct cctcttatcg gggctgcctt cgccggtatt
    10981 ctttacaagt ggtttgaaga agaaaactga gttcagactg aacattggat catgatacct
    11041 ggctgaatgg taaaacagag tatattacga cggcaggaac ttctcctgcc gtcccttttt
    11101 tttacgatac ggaaacctgt tgtaattcag tcaccccaat tccgtgcgcc tctgccatga
    11161 caacccattt gctggaaaaa aaggattctt gccagattgg cggtatttcc ttcaagtttg
    11221 ataagaaggc aaatcagttg aaaagtttgc agaggtctac aagaacgtca aggcggagga
    11281 gactgccaac cgactcgaac aattggggca tcaataggcg ctggcagagc aggacgttcg
    11341 actcccctac gggataggca ggaaaaatcg tcgcatgtcg atgaaaatca aggggtggaa
    11401 agtggcagcc cgtaggggat tcgaacccct gtgacgagac tgagaatctc gcatcctaac
    11461 ccctagatga acgggccgca tggggactgg caaaaggaaa aatgccagag aaggaaaatg
    11521 gttgccggac taggattcga acctagaatt gcctgatcca gagtcaggag ccttaccaat
    11581 ttggccatcc ggcaacagca ggtatatttt aacggtttcg tgcatcagga atcaaatcag
    11641 gctccggttt cgcatccatt ttactgcaat gccccggcag gcttgagggg cgcccggatg
    11701 accgttctcc ggtgcacgca aaggcttcaa tatactattt gtgggccgtt gccatggctt
    11761 ttgccgcttc agcaattttc ttgcgtaagg atactggctc taccagttcc atggcgccgc
    11821 cgaagctgag cacccaggcg agcgtttctt cctcgctggt agtagaaaaa tggagcgtga
    11881 gagagccgtc tgcgtggttt ttgattttct gatccccgct ccagacgcgt tcctgtacat
    11941 acatggcagc agtggctgcc acttttacag ttgcgcgaaa tgcttcaccc tgacgcagtc
    12001 cgaaagtgcc tgcgatatcc tcggcagagt cttggggtgt aatggcttcg aaaaactgat
    12061 cggtgagcga tacgctgtgc atgcgatgaa cggagaggat aatatcttcc ggcttttccg
    12121 cttcgagttc tgccacgggg cggcaccgga gataaagccc ttccctgaag acgaccatct
    12181 gatatccggc tacttggtag ctttttgcag ctttgcggga ggggcttttg tacgagattt
    12241 cgcagatacg gttttcgcgg atagcacgga tcagatctga aataaactgc tctcttgagg
    12301 agtaatcaat gtagcctttg ggcttcactt gtgtgaagga gtcgggcact atttgtggtt
    12361 ctgcgtaggt ctgcttggcg gcatttttta atgcggcagt aatctctttt cgataggcat
    12421 ccggcaggag atgccaaacc atatctttac aaagcaggag tctttctatg gctgtactat
    12481 caagaacaac attgctgggc tttttgacga tcctggtctg ataccatcgc tgctttttag
    12541 tgatccacga atcgaccgtg acccagtcgc ttttctgtat ctgttcaaat atccgcagaa
    12601 cactctgttt agaacattgc agaatggccg ccagttgtgt cagcgaatgg gggcgctcgg
    12661 taaaaagaaa aatactcaaa agccccatca ttttttcact gggacttgca aaaggattgt
    12721 gtttgggcgg cattgatttt ctcctaacga tttttcgcca ttcagctaaa tactgaataa
    12781 aacttcatta caaactgtat cataagcgga caaaaaaaac aatttcaatc ttttcttacc
    12841 gtgagttcaa tatctaaatt gggctctaac gatggttgac aaaaatgcat aaagctgtta
    12901 gactagtacg tgtgagaaaa aatgaaccct gagaagtcgc tcatccgggc tatcattctg
    12961 gaagagatat tcattcctgc tactaaactc aggcacctgc ctttgcagga atcaatctgc
    13021 atcattagtt tcaggaggtg gtggttcatg aaacatttaa ctggatttgc acttcttacg
    13081 accgcgctgt tattgaccat gctgatttcc gggtatgcag aagaccgacc ccttgaaatt
    13141 tatgttctca tgaaaacgga tccagagtcc cctgagcaaa tggtcaaagt atatgttgat
    13201 tatgttgaac cacgagatcc ggaaactcca tggtgtcacg acccggatat ttccgccatt
    13261 caagacaaac gaatagtggc attgtgggaa gatatcataa tctcttatcg gcgaaatcat
    13321 cctaccggcg caaccgttcg aggactcgat gatgcgccgg agtttattgc ggagcctaat
    13381 actcctgaaa accgggaaat attgatcgat gcactttctt tggcgctttc agccgacccg
    13441 gttgactgga attatgtgct taccgtattg gtgctgatgc aaaaaggaat ggcaccggac
    13501 ctgcggatca cacaactggc tgatcggttg tttactgccc gccgcaatga agaaatgagc
    13561 tatgaccacg gtgaatgtat tagaaccatg tatcgtgttt taaagcggca aaagaccaaa
    13621 gaggcagcag aattgttgta cagagcctgt tcacgggact attggggcga agatccaatg
    13681 cttaccctca gtttcagcga gaagtggtcg gagcattcca ttatcacggt acgaaaattc
    13741 gctctgagct gcttgtcaga agatatgccg cccggattaa gcataccgct gcttgaagaa
    13801 ttggcatcag tgtatccgga tagttatgtt cctccggata ttattgtaca gcctgcggat
    13861 ggactcagat cggaacggcg gataattatt gatttctccc gatcagatat tgaggaggca
    13921 ctcgaaaagg cttacaagaa acaggcagca gaagaatccg gaaactaaat tatcgttaag
    13981 ataattcgcc agatgtcgac ggtagtatta tgcagatacc gcttacgttg ttatgacgca
    14041 gaaagaatat ctcaaaatgc tgcgtagatt gcccgctgaa tcggcaatta tggaatagtt
    14101 ctaaagggtt ctgtctgcat tgaaggggtt caggttcatt gtcagccgta tgccggatct
    14161 ggcaagggaa gtaagtaaca cacccattct gacatacatt cttttgttgc cacagaattg
    14221 actggaatag cgcacgtttt attacgcgat ttcagaaaac ggctgcgcat gaaggggaaa
    14281 tgctcttctt gagtgaagcc ggggatgatg caggggccgc tgtgttttat ggggggacat
    14341 tacagaggaa cagaccgggg ctccttccgg aggccctaca ggctgacatg cccagcgctt
    14401 tctgttagtg gctgcactca aaccagtttc tctcccgttt ttttcctcat atttatggta
    14461 aaagcccaaa tgtcattaaa acagcataaa atattgtatc aattcgtcta tatggaacaa
    14521 atgtgtggca aactaatgcc caaagttgaa tttaggttac tccggtgagg ctcattttgg
    14581 gtgtggcacg ggagcaggaa gtgggaacct cgggaacaga cggggtccgt gtcacacctc
    14641 taaacagaaa gagaccgaca ggccaggcgg cacctgtcgg tctcgaaagt tttagcagta
    14701 aaaagaatta gataccgaag gcatcaaatc ccacaagtac ccatttccca ttttcattct
    14761 taaggacaaa ttccagggtg ccgctgccaa aattgccgga gatatccaga ggatagacgg
    14821 ttgccgtgtc cccttctatg gttacttctg tatcttcgag atcaatactg gcatcttcca
    14881 gaaatcccat ggcaatggca gattcgagga aatcctttaa ttcggcttta tctgcgtact
    14941 cagagttctg aaagtcttca gaaacccctt ccagagcagc atcaaggttg ccggcaaaga
    15001 gtttgttgag aaactcggtg gcacgggcaa gaattgcttc cggtccggaa ggcgctgcgg
    15061 caggagctgc tgctgaagcg ggcttggcat ccgggcaggc agcagcgatg gggcagtcag
    15121 cagcggcagg gcaggcagca gcaatggggc agggcgctgc cgtgcactgg gaagctgcaa
    15181 gtttgccggc aagttctgct ttcacagcgt caaaattgct gaccatcagg tagagttctg
    15241 caacttcggc ggcactcata atatcagcag cctcgtccat ggtgctctgc ttgaggcgga
    15301 ggatgctcaa atcaagtgcc ataagttcgc gtgtaagacc ggaaacaacg ttgtcgctgt
    15361 cgtcgttggc aatggcggcc ttcaaagcag cttcttttgc agcacgctgc ttggtataag
    15421 catccatggc agaacgatag gcgcgatagc cttcgagcac ttcaatcaga tcatagtcat
    15481 caattccggc tttttctgca aactgcagaa gaagaacctg ttccaggagg ctgtccggac
    15541 acgcctcttc agcgtaagca ccggcagcga aaataaaggc agcagcaaga cacacagtaa
    15601 ccagtttttt catacgtcaa tctcctgttg gtttaaactt ggttaaataa gtggcagttg
    15661 ttcagatttg actgctacac gacgtgaaag gtttcagaaa aaaaaatcgg aaaattaaaa
    15721 taaaccggcg tttttacggg cgcggaggcg caaggcattt aagcgaataa atccggtcgc
    15781 atcggtctgg tcgtaagcgc cttcgtcttc ttcaaaagtg cagattttag ggtcgtacag
    15841 tgttttttcg gcacggcggc cgaccacatc gcaattgcct ttgtacaggc gcaatcgggc
    15901 tgttccggtt acggtctcct gacttttatt gacaaaagcc attaaggcct cgaattcagg
    15961 ggagaaccag aagccgttat agatcaattg cgcaattttc ggggaaagaa tatcccgctg
    16021 cagcatgact tcccgatcca tggtcaatgt ttccacggca cggtgggcgg cataaagaat
    16081 agtgcccccc ggcgtttcgt atacccctct ggatttcatg ccgacaaagc ggttttcaac
    16141 cagatcgaca cgcccgatac cgtgagcacc gcccagggca ttcagagttt ccagcaattc
    16201 tacgggcgcc atccgttttc cgtcaacagc gacaggcgta ccttcttcga agtcaatttc
    16261 cacatagtgc ggtgtgtccg gcgcctttaa cggcgacacg cttaattgaa acatgtcttc
    16321 gggcggttcg ttccagggat cttcgaggat gccgccttca aaggagatgt gcagcagatt
    16381 tctgtcggag gaataaggct ttgctttgct gaccggtaca ggaatattgt gtttttcggc
    16441 ataggcaatc atcttttccc ggctgtccag tgtccatttg ggatcacgcc agggcgcaat
    16501 gatttttacg ttgggctcca gagcaaaaca ggtcagttca aaacgaacct gatcatttcc
    16561 ctttccggta gcgccatggg caactgcatc agcgcctgtt tcgcgcagga catcaatctg
    16621 cgctttggca atgatcggac gggcaacact ggttcccagc aggtacacgc cttcatacac
    16681 agcattggca cgcatggtcg gaaacacgaa gtcccgtaca aattcttctc ggagatcttt
    16741 gacaatggcc tgagaagcac cggtattcag tgcttttacc cgtgccggct ccgtttcttc
    16801 tccctggcct acatcgccga tgtaggcgat gacttccgca ttataggctt cctgcagcca
    16861 ctttaaaatg atagaagtgt caagaccgcc ggaataggcc aacacaatct ttttgactga
    16921 gctcatcagg aattttcctt tcttgaaaaa atattttaat gggtcttagg atacaccgcc
    16981 catgagagtt gcaagaactg ctttctgggc atgcaatctg ttttcagcct gatcgaagca
    17041 gacggagttg gggccgtcta taatttctga cgtgacctca gcaccgcgat gtgccggaag
    17101 gcaatgcata aaaagggcat ctttttttgc ccagcccatg agtgtgctgt ttacctggta
    17161 gggagcaaag atcttggcgc gttctgctgc ctcgtcttcc tgacccatgc tggcccagac
    17221 atcagtatat acagcatcgg cgtctttcaa tccggcttcc gggtcctgag ttataacaaa
    17281 ttctgcccgt gtttctttcc gtacccgctc aataatttca ggatcgccct cgtagccttc
    17341 cggacagacc agcgtgagat tcagggggag tctggataca aaatttgccc aggagtggaa
    17401 aacgttattg ccgtccccga caaaagcgac tttgagcgag gaaaggtctt tgcctttatg
    17461 ttcccgcaat gtctgtacat cggcaagtat ctggcaggga tgcagtttgt ccgtcagtgc
    17521 atttataaca ggaatcgagg ctttttctgc aagttcaatt acatgcttat gggcaaaagt
    17581 ccgcgccatg atgccgtcga cccagcgttc caggttccgc gccacatcgg cgacagattc
    17641 ccgttcaccg agtctggtat cgataagctg cgtggttccg cccagctgat acattcccac
    17701 ctgaaaggtg agccgtgttc taagacttgg tttttcaaat ataagcgcca gggtttttcc
    17761 gttgagcagg ggatggggaa tgccggcctt tttctgcgct ttgagttcat cggcaagatt
    17821 taaaaagtgc attaactcat ctgcggtgaa atcagccaga gaaataaaat cttttaccat
    17881 tgcaaagcct ccagagaagc ctccagtgct gcgataacac aatcgacagc ggtggaatca
    17941 ataataagcg gaggcagaaa gcgaagtaca cggggaccgg caataccgca caggatccct
    18001 gcatcatgca tcgcagcgac cagcggggcg acttcacggt caaattcaat cccgatcatg
    18061 agaccccgtc cgcgcacttc gaggatgctt ttgtgttcca gcgacagatc caccagctgg
    18121 cgaataaaat acgcgcccat ttctgcagat ttttcaacga gacccggctc tgtaagtact
    18181 tcgaaagtgg caagagcggc agccgcactt aacggattcc cgccgaaggt acaggcgtgc
    18241 gcaccgggag agaacccttc ggaaagcttt tcggtacagc ccatggcacc tataggcacg
    18301 ccattgccca acccttttgc cagtgtgatg atgtccggta tgacgttttc atgcgcatag
    18361 gcaaaaagtg tgcctgtcct tccgagtcct gtctgcactt catcaaaagc gaggagaaca
    18421 tttttatcgg agcagagccg ccgtacaccg ggaagataac ccggggaggg aacgcgtact
    18481 ccgccttcac cctgaatggg ttcaagaata accagaccga cttgcggtgt gatggctgca
    18541 tccagcgctt tcagatcgtc gtaaggcaca taatgaaagc caggaaccaa cgggtcgaaa
    18601 ccgtaatggt atttgggttg tcccgtggca ctcaatgcac ctaaggtacg tccgtgaaaa
    18661 gagtgctcag ccacgacaat ttccggttta ttggccccga tcattgtcca gtagcgccgg
    18721 gcaagtttta tggcggcttc attcgcttct gcgccgccat tgcagaaaaa ccatttatct
    18781 gcgaagcagt gtctggaaag tttttctgcc agtttcacct gaggctcaat ataatagaga
    18841 ttggaaacat gtaccagttt ttttgcctga gtacagatcg cctcagtgac tttggggtgg
    18901 cagtggccca gattacatac ggcgataccc gcaaaaaaat ccaggtattc acgtccttcc
    18961 gcatcccaga cacgggcgcc ttcaccgcga accaacgcaa gccgtctgtc tccgtaggtg
    19021 ttcaaaatat gctcggtgtt gagtgttttt atttcctggg tgttcaacgt ggtcatgctc
    19081 ctgatacaaa aagaaaaaat taggaagcct ttttatcgct tgatgaaacg gtgcctgagg
    19141 tgctctggtc ggttttactt tcggaagaag atttagtggt agctttggaa gactttttat
    19201 tgctcttgtt cttataatca gtttcgtaga agccggtacc tttaaaaatt atgccggctc
    19261 cgcttcctaa aaggcgtgtg caggcaccgc cgcattcagc gcattttacg cgaggcgact
    19321 gagaaatgcc atggaaaaca tcctgcacgg cagaacagcg tttacattga taggaatagg
    19381 tgggcacaat acaatccttt acaacacagc ttcgggggaa aaaataccga accttaagtg
    19441 tctggtattt ggtaaaatag gagatgctta tcgctttcag aacttaatac tgtaccaatt
    19501 tacggcgcaa taatcaaaaa aag
//
LOCUS       DAOPWB010000055        19493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2829_length_19493_cov_5.009209, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000055 DAOPWB010000000
VERSION     DAOPWB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 19493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19493
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2829_length_19493_cov_5.009209"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3255)
                     /locus_tag="PKN92_06830"
     CDS             complement(<1..3255)
                     /locus_tag="PKN92_06830"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNZ48542.1"
                     /translation="MGINLRARFSICASLWCLLIFIFLSGSTFAADPTDLLKLTSEAR
                     PWVYWMWKNGNISREGITADLEAMEQAGIGGAIVMEVALSVPPGPVSFFSDEWRAHFA
                     FALQEANRLGLQMVINSAPGWTGSGGPWITAEKSMKKLVFSEIRVTGPMHFKASLPQP
                     ESVCDFYRDIAVLAFPEPQPGAHIEALEEKSLYTRAPFSSMPGVRPYFDPVADYPSAD
                     PSQCVPPKSVRNISDDMSSDGTLVWDVPEGNWIVLRAGYTSTGQTNRPSPLPGLECDK
                     LNPVALEEHFNAYISKLLQDSGDLAGKTLAGIHFDSWEVGGQNWTDNFQDEFEKRRGY
                     DILPWLPVVSGRILDSGTSSERFLWDLRQTVSEMIVEYHGLQMQRLAHDSGLKLSIEA
                     YDMTPCGNMNLSAAADVPMCEFWSDTFDTRYSVREAASAAHIYGKPIVAAEAFTSVDK
                     WLLHPGAIKTNGDWAFSEGVNRLVIHRYIHQPFMQIRPGLSLGPHGLHYERTQHWWKM
                     ARPWHEYLARCQNALRHPAEADLLYLSLEGAPNVFQGPEPAPRGYKYDACTADALLRR
                     VRCEDGFLVSDGDARYRMLILPDSEIMSVPLLEKIAEMVRNGALVVGLPPLRAPGLSG
                     FPEQDEKLRKLAAELWPGERRPAGFQEISFGKGKIFWGGDLHPDSQLREEKNPLADAE
                     WIWCDAEDALAASPGKRFFKKEFLVAEEDMPEEAVLSATADNAFRLWVNGAYAGEGTS
                     FTIIYSFDVLEYMRPGKNLIAFEVENTGEEANPAGLVAALHILCRSGKEEVLITDASW
                     FSAREVAPGWQETSVNPAEWQSVQVLGYMGMMPWGLIKLAPKRPSLYPPSAALEALLQ
                     DLGTPPDFEAPPALRFIHRRTVEGALYFVSNGSASAVDAEVLFRAADGSPLLLHPEDG
                     SVRTLPEYTLLEDGRTSIPLHFEEGESYFILFSSKPESTARVAPKNFVAYEKLAEISG
                     PWQVSFDPALGGPEEPVLFSELTDWSLHTSPEIRYYSGPAIYSCDFEVGEGMAELPGL
                     QLDLGTVNVAASVQLNGQDLGICWKKPYRLPLGASLRAGMNHLEINVVNLWVNRI"
     gene            3491..3670
                     /locus_tag="PKN92_06835"
     CDS             3491..3670
                     /locus_tag="PKN92_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016812228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48543.1"
                     /translation="MYQQQAHAYLDPSSGSVLIQIILGGVAGFFVLLKIYWRRIRRAL
                     GLYKEDSHPENVSDE"
     gene            3663..5075
                     /locus_tag="PKN92_06840"
     CDS             3663..5075
                     /locus_tag="PKN92_06840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ48544.1"
                     /translation="MNEGLFHQEPGSFRDRNNQVYMVDGRIVRGISSTARAHWEALKE
                     SSFYKKHSAAGRIVATREIAPDNLPSPFTEEQLWDCYLEHDRLPFISYVYEWCFGMLK
                     DAALLHLDLMEGALREGLILKDASVFNIQFSGAREVFIDLPSFERYEAGMPWVGFRQF
                     CQHFLYPLMLQSYKDISFKNWFKGNVDGITPKECNNILSFRDKFRKGVFSLVYLQSKL
                     VDSMGNAKRSIVSEAQDSDFGKEIILANVKKLRKIICGLEWKPARSEWSDYTKTHSYD
                     DTCFEQKCRFVSEAVAEKPLKLAWDIGCNTGHFSRILAKNSAYVVALDIDELSVQRLY
                     ESLRQEGTDNILPLVFDLTNPSPPLGWRCRERHALEQRGAPELVLCLALVHHLVITGN
                     VPLREVIEWLASFRCAIVIEMLTKEDDMVRKLLLNRVDQYPEFTIDGFESLAAPYFTV
                     KKKEEVMAGKRFLYHLLPRE"
     gene            5300..7090
                     /locus_tag="PKN92_06845"
     CDS             5300..7090
                     /locus_tag="PKN92_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ48545.1"
                     /translation="MKSCRAFRTLSAVFAFIVLCVFSAAGNTLTDQSLARLAAKPPVC
                     DDVVDFVVCADPQPGGLLGTPQVFLDMLDEWNVLQPDLVMCVGDMIMGGPADVVGSMW
                     DEFLGNVGKLQVPFFPTAGNHDVNPEPAVMSTYEERVSPFYYSVVRGNSEFIVLNTEE
                     PGNPDGFSPSQRKWLEKTLVASKARHIFIFLHVPLFRGNWARDWQHMEDLFVQYPVRV
                     VFAGHEHLYRDWGTHKGIRYVVAGSAGGGFRTPEEEGGMFCYLWVKVRGEEVSWSVVR
                     PGAVLPSDVVTETNVQKLHALQKILDIEEIAVHWNSTLDQKIEVRLHNPFSEEISSTF
                     RWEIPEGWTVTPASHTFKVAPGTTEVQQCHVKTDGAFRFPSPVLQGKVEAEELVRTLE
                     LSPSLDVKPQASAPHAPGKVVLDGDLSEWSGAVPLPMLYGSSYDPAETEDLQAQARLM
                     WDENNLYIAVEVEDNEFHQPHYGDVVWMADSVELWVEDSNWSFSLTESGPQVFLDERP
                     DKHLDAVIDGVPLAVKRDGRRTVYEAAYPAEELPQIACRAGSVIRFSILVNDLDPSGP
                     LTKRHYAELTPGAGAHFDCAKYFITLEAAR"
     gene            complement(7111..7788)
                     /locus_tag="PKN92_06850"
     CDS             complement(7111..7788)
                     /locus_tag="PKN92_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48546.1"
                     /translation="MHMRSLPDFLLRCSFVAVFLTASVQAHALDSPVIINCGTATKLT
                     LPTHVEFLFNREFGGESLSEAVAQCDAYRHAALAQIRDAELQPTDIRMTPVLPVSSCM
                     VSSGILVQFSMSSFNTPSGAALFAALSDRLSLLASEMQASLEDPFFFTMQKEAIEAEV
                     VARATENAFPHAEAVSEALKSTIYAIEKVEIKEVVWEQQPQKQYGESAQIGCTAKVQV
                     TYMMTTQ"
     gene            complement(7804..8625)
                     /locus_tag="PKN92_06855"
     CDS             complement(7804..8625)
                     /locus_tag="PKN92_06855"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNZ48547.1"
                     /translation="MNLVCFGAHPDDPEVFAGATMIRFAEAGHRVLAVSLTNGDIGHY
                     ALGGGMLAARRLAESARAAEIGGYESLVLDHHDGELMPTLELRKEIVRIIRRCRADIV
                     FTHRPNDYHPDHRYAAQAVQDAAFMVTVPQFCPDVPALQKNPLFLYLFDPFTYPVPFR
                     ADVALPVDTLMPRKWQLLDAMDSQFYEWLPWLEGKAGEVPPADADPAERLFWLKQFFS
                     PELELPVKLHGDALAEWTPAGKASCRFAEMFQVCEYGNQPDKQRLQDIFAPMLKI"
     gene            complement(8627..9295)
                     /locus_tag="PKN92_06860"
     CDS             complement(8627..9295)
                     /locus_tag="PKN92_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48548.1"
                     /translation="MRKFLVFATAFVLMFAGCGRDSEKDPSRAAAPVAAGTAVQSTTM
                     PSKPPIDLGDVDSGLTIRAELARGAASSTVSADYVETLRGSLSMATITVEEPFPETLP
                     ISFSLFSQRPFVERPVVVRIHAYRDDERITEESSAWIMGRDARQEPVGADGKPRPRTF
                     TVDVMAGLESVPETMLVHARADAWLMETGTAEDLLDPKTATSAEQVAIMSNPVRINFK
                     RAVQ"
     gene            9410..10924
                     /locus_tag="PKN92_06865"
     CDS             9410..10924
                     /locus_tag="PKN92_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ48549.1"
                     /translation="MALSRHIFCVALLFTLPFFLMTAAAASDQPQWGQCFSRNMVSEE
                     AGLPASFDPQTGHNVLWTAPLGGGCYSSAIVAEGCVFIGCNNSDPKDPHQQGDRGTLY
                     CLNEKDGSLKWQLLVPRIGGDDFLDWPGIGICSPPTVENGRVYTMTNRTEVVCLDLAG
                     MTDGNGGVFQEEGRHMTPAGSPAVEPGAADADILWLYDLRNELGVYPHDSPHTSILID
                     GDFLYLNTCNGTTNTHREIGNPEAPAFIVLNKHTGRLVAQEREGMSRRMFHSTWAPPS
                     LGEVQGKRLIFFGGPDGICYAFHTLKQQVYETVQDLELCWRFDCDPTAPKENVFDYLN
                     NNKEGPSLIMSMPVFMNGRIHVTAGGDIWWGKRQSWLKCVDPAGTGDVTAAKEIWSYE
                     IPYQCSSTPAVTADLVFVGDDGGQLHCVDAKTGGACWVHKFGRKIWGSALVADGKVFA
                     AGRNGTLAVFENSREKKLLWETRFPEEINGTPTAANGVLYVATLSRLYALKAMD"
     gene            complement(10926..12311)
                     /locus_tag="PKN92_06870"
     CDS             complement(10926..12311)
                     /locus_tag="PKN92_06870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ48550.1"
                     /translation="MKITSFGAAGNVTGSKHLLEINDHRILLDCGLFQGSREQADRKN
                     RESLFDPSTLDAVVLSHAHIDHSGVLPLLIKQGYTGHIYSTHATYNLCNIMLLDSAFI
                     QKRDAEWLQKKHRSFIAPLYDENHVYATMKRFVSYSYEERFEVAPDIYVTFHDAGHVL
                     GSAMVEVQYREKNRDRRFIFSGDIGRKNMPLLQDPWEPDDADVVMMESTYGDRDHDPI
                     ETNEAKLAEVVLRTVNRGGKVIIPSFALERAQEVVFCLKRLEMSGTIPEIPVFVDSPL
                     TVNITEIFRYHTENFDDEFRQVMQEGGDPFLLKHIEYIRNMDDSLRINSFKGPCIIIS
                     ASGMCEVGRILHHLRNHCENPKNTIVIVGFQAQNTLGRRIVERQPEIKIFGVKRPLNA
                     EVKTLNGFSAHAGRQELLEFGARFRRRPKNILLVHGENASLQALKSGLEEQKARGVKI
                     MREAQPQRFDL"
     gene            12577..15084
                     /locus_tag="PKN92_06875"
     CDS             12577..15084
                     /locus_tag="PKN92_06875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ48551.1"
                     /translation="MSTTPPAGSIPVIYAPPPQSDGTVQQYVMVPVPAPQSPTKSSKK
                     ESAFAWLVLFLILTVHLAANLYWLHTDRHLIRMDEAHHMKQSLAYHDALFTEDTGSTA
                     EGILAALSIESPYPPLLHIIGAICIRYLGGSPDSAALAGSIALALLLIGVFVLSRLVL
                     ASQAALLVAVVTGFTPMVFGYARLMIPDTFMAAWLVWCLAALIRTGAFHKSGWSLLCG
                     FFAGLAMLSKQNTLIYLSPLLAGAVLWGLKKAFSERKRDGADKYTFSRILVNLLLFSC
                     TFAAVCGWWYIRHAEYLYGWWSSASEENASVFQEPAASLFAKVLPPSGAVPALVTDPD
                     LPVPYKEIPASDKGSGIAFFSDALYRYGTMNLFYLINSVLFLPLALVAGLGVLSLLLK
                     KNRNSLSLIFLLWVAAVWFILTGLFALQSPRFLYPLIPALSFFVYLALNGLSATRAFR
                     AAWALVFIALLLQFINLSFVPAPYAMHLKLPFFQNTEEAEENDVQPLTVLSGEVAAGN
                     YIIQAPVTEIPVSEKLVAAMEAHYPPDATVAWYQIIGDMPDALGVDFYAHSFPHRSLF
                     DCFLEADAPGKHPVFSALNEAAAELSAHPADTAEADFIILQRAKASQTEPLLEKDAAL
                     LMKQGFQMAFHAVLPGAEKQQPAQVLLMARTTTLTPGACTDLFSLYDLLERDGREWVL
                     TDEERRDAESRYARQISAYAVPRPLKQGIHFMGVHIAQAAENAYLIRIILHVQQTPQE
                     KLRVWMRGISETAGAQAAISEAASWDFDPDPPSMQWGENEAIILSRPVLVSPAVFHLE
                     IGLFNPETADRPLALLETETVDFAETDRLDRDYREAL"
     gene            complement(15079..15444)
                     /locus_tag="PKN92_06880"
     CDS             complement(15079..15444)
                     /locus_tag="PKN92_06880"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNZ48552.1"
                     /translation="MFTVIAFVLLSHQPMQPVVEGLASYYTVRGTGGITASGERLKDN
                     AMTCAMRTGEFGDYVLVVAENGKSVVCRINDRGPFVKNRVIDLSRSAMKALHPTAGLV
                     PVKVYNLGSEPPGLNQAPL"
     gene            15977..17620
                     /locus_tag="PKN92_06885"
     CDS             15977..17620
                     /locus_tag="PKN92_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HNZ48553.1"
                     /translation="MCAQEHHDHSSAHSHRCSGCCGTRFSELSRRQFIAGTGAVALGS
                     VFMHHLQGRTAFADTVPRRILKQDRPLRIQPVLTCSLPRYSEATSWRPWGGLLTDSHV
                     REECAKIQDELNAMAAKADFPLELLPLKTVQSAEEGRSIAAEDHDGTLIFGAAGGTDV
                     IISLADPKKHNLLFVRHKSGPVYLWFEIADPRFLRQESDFPKGTGGMDHNDVIVDDFD
                     EILWRLRALHGLKNIRNKRIVVIGNAIGWDRGTASDSPKRAQELWGLDYREIPYTEMG
                     ERIRAARANDALVRQCRDMTEAYLRQPGVTLLPRQQQLTTRELIAGAGSSDTLQEIRV
                     FTEKAFLLTEVFRDLMEEHETDALTVSECMGTIISMSETTACLCLTLLNDEGCMAFCE
                     SDFAVIPSGILLHYICCKPVFLCNPTFPHNNMITVAHCTAPRKMDGVNAENMFVRTHF
                     ESDYGAAPKINFKLGQNMTILAPDFASRRWMGFEGMVLENPELDICTSQVDLRINGDC
                     DRLLHEKAGFHVMACYDNYLREVGYALHKAGVEWVNLSA"
     gene            17860..18177
                     /locus_tag="PKN92_06890"
     CDS             17860..18177
                     /locus_tag="PKN92_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48554.1"
                     /translation="MKTVYVVTMWSGGRPAKKWKTEHAPELLAQGTGISFINAGTKMQ
                     VSVIGSISVEEYESGKEELEMGLNPTPGITSDFSQGKPDNCESLSDFKRSKKNIFTDE
                     VQE"
     gene            18222..>19493
                     /locus_tag="PKN92_06895"
     CDS             18222..>19493
                     /locus_tag="PKN92_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNZ48555.1"
                     /translation="MADKLSNQHFESRPRQLIQRHWLLFFCGEASGILWALFLVDTAA
                     LTIAVVFLLCCALYFYPGLLPGRRLVIWFLLFFAFGNLLVVFHGLPRKADKLHELALS
                     YPRGMMEFEGTASDTSPFLSGRTAYSTCVLHVDTLVKDKASMPLSGKAVLRWSEPSCA
                     LFNGSRVRVIGRLSPHLSTVNYGVTDSEDRRRRQGVFSGIHTRNGQVQIIHDPGFTLA
                     GMASRLRQSQAEHLVKTVPEEVFPFVLGIWLGDRSLITDETYQIFAWSGTAHVLAVSG
                     LHVGVIALTFPFLCRLLRLRKRYTLLCSMAGILLFTLMTGAHISVIRASLMMLLYYLY
                     QLLRREPDTISVLSLSGVIFLTIQPHVILDIAFLLSFGSVSSLLLFYPGILARLQNLP
                     RFLSSACAVTLASQVITVPIIAWSFFTVSLTG"
BASE COUNT         4543 a         5197 c         4967 g         4786 t
ORIGIN      
        1 aatccggttg acccacagat tcaccacgtt gatttccaga tgattcatac ccgcacgcag
       61 ggaggcgcca agaggcagtc gataaggttt tttccagcag atgccgagat cctgcccgtt
      121 caattgtact gatgccgcaa cgttgaccgt gccaagatca agttgaagtc cgggcagttc
      181 agccatcccc tcccccactt caaaatcgca agagtaaatc gccgggccgg aataatatct
      241 gatttccggg gaagtatgca gggaccaatc cgtcagttcg ctgaacaata caggctcttc
      301 cggaccgccc agagcgggat caaaactgac ctgccaaggc ccggaaatct cggccagttt
      361 ttcataggca acaaaattct ttggggctac gcgagctgtc gattccggct ttgaactgaa
      421 gagaataaaa taactttcgc cttcttcaaa atgaagcggg attgatgtgc ggccatcctc
      481 aagcaatgta tattccggaa gggtgcgaac actgccgtct tcaggatgaa gcaaaagcgg
      541 cgatccatcg gctgcgcgga agagcacctc ggcatctaca gcggaagcgc tgccgttact
      601 gacaaaatac aacgcgcctt ccactgttct tctgtgaatg aaacgaagcg cgggcggtgc
      661 ttcaaaatcc gggggagtcc ccaaatcttg cagcagcgct tccaaggcag cggagggcgg
      721 gtacaggctg ggacgcttcg gagcgagttt gatcaagccc cagggcatca tgcccatata
      781 ccccaacact tgtaccgact gccattctgc aggattcacg gacgtctcct gccaacccgg
      841 agcgacttcc cgggcactga accaactggc atcggtaatg agcacttcct cttttccgga
      901 tcgacagagg atatgcagtg cggcaaccaa gccggctggg ttggcttcct ctccggtatt
      961 ctccacttca aaggcaatca gatttttgcc ggggcgcatg tattccagca catcaaaact
     1021 gtagataatc gtgaaactgg ttccttctcc tgcataagca ccgtttaccc acaggcggaa
     1081 tgcattatcc gccgtggcag acagtacagc ctcctcaggc atatcttcct ctgcaaccaa
     1141 aaattctttc ttgaaaaaac gctttcccgg agatgctgcg agagcatcct cagcatcgca
     1201 ccagatccac tcggcatcgg cgagaggatt cttctcttcc ctgagctggc tgtccggatg
     1261 aagatctccg ccccagaaga tttttccttt accgaaagat atctcctgaa acccggccgg
     1321 acgccgttct ccgggccata attccgctgc cagtttccgg agtttttcat cctgctccgg
     1381 aaagcccgaa agaccgggcg ctctcagcgg cggcagtccc accacgagcg cgccgttgcg
     1441 taccatctcc gcgatctttt ccagcagggg aacgctcatg atttcagaat caggcagtat
     1501 cagcatccgg tagcgggcat cgccatcact tacgagaaag ccgtcttcac accgcacacg
     1561 ccgcagcaat gcatcggctg tgcatgcatc atatttatac cctctaggtg caggctcggg
     1621 cccctgaaaa acgttcggtg cgccttccag actcaaataa agcagatccg cctccgccgg
     1681 atgacgcagg gcattctggc agcgtgcaag atattcatgc cagggccgcg ccattttcca
     1741 ccaatgctgg gtgcgctcgt aatgcagccc gtgcgggccg aggctcagcc cgggtcgtat
     1801 ctgcataaag ggctgatgga tgtaccggtg gatgaccagc ctgttcacac cctcggaaaa
     1861 agcccagtct ccgttcgttt tgattgctcc gggatgaagc agccatttat ccactgaggt
     1921 aaacgcttct gcggcgacaa tcggcttgcc gtaaatatgg gcggccgatg ccgcttcacg
     1981 aacactgtaa cgtgtatcga aggtgtcgct ccagaactcg cacatgggga catccgctgc
     2041 ggcactcaag ttcatattgc cgcagggtgt catatcatag gcttcaatag aaagcttcag
     2101 ccccgaatcg tgggcaagac gctgcatctg taatccgtga tattcgacaa tcatctccga
     2161 cacggtctgc cggagatccc acaagaaacg ctcagatgag gtgcctgagt ccagaatcct
     2221 tccggacacc accggaagcc acggcaggat atcatagccg cggcgtttct cgaattcatc
     2281 ctgaaaatta tccgtccaat tttgcccgcc cacctcccag ctgtcaaaat gaattcccgc
     2341 aagcgttttg cctgcgagat caccgctgtc ctgcaacagc ttacttatat aggcgttgaa
     2401 atgctcctcc agtgcgacgg gattcagttt gtcgcattcc aaccccggca gcggtgaagg
     2461 tctgtttgtc tgaccggtac ttgtataccc tgcccgcaac acaatccaat tgccctcagg
     2521 cacatcccag acaagagtgc catccgaact catatcatca ctgatattcc ggacagactt
     2581 cggaggcaca cactgagacg ggtctgcgga cggatagtcc gctacgggat caaagtaagg
     2641 gcgcacaccg ggcatggagg aaaaaggtgc tcgggtgtac aaagattttt cttccagcgc
     2701 ctctatatgt gcacccggct gaggttccgg aaaagcaagt acagcaatat cccggtagaa
     2761 atcgcataca ctttccggct gcggaagaga agccttgaaa tgcataggtc cggtcacccg
     2821 tatttctgaa aaaacaagtt tcttcatcga tttttcagcc gtgatccagg gacctccgct
     2881 tccggtccaa cccggggcac tgttgataac catctgtaac cccagccggt tcgcctcctg
     2941 cagagcaaag gcgaaatgcg cccgccattc atcgctgaaa aaagaaacag ggcccggcgg
     3001 aacgctaagc gccacttcca tcacaatggc tccgccgatc ccagcctgct ccatggcctc
     3061 aagatcggcg gtaataccct cacgactgat attgccattc ttccacatcc agtaaaccca
     3121 ggggcgcgcc tccgaggtca gcttcagaag atcggtggga tcagcagcaa aggtactgcc
     3181 tgacaagaaa atgaaaatca gcaagcacca caaagaggca cagatagaaa agcgagcccg
     3241 taaattaatt cccatgacat ggctctcccg tttcaggatt gatgacaaga taataagtaa
     3301 cacacggaga acaatacaag gacttgtggc ggtcggcgtt aaaatcatag ggctccatct
     3361 ttggtattct tttcctttac aaggtacaat agatcacctt tgtgttcttt tccaacctcc
     3421 aaatcagaaa aagagatgtt ttatgaatgt ccggttcctt tttctttcag tgctatttgc
     3481 ttcccttttc atttatcagc aacaggcaca cgcctatctg gacccctctt ccggatcggt
     3541 actgatccag attattctgg gaggtgttgc cggttttttt gtgctgctga agatttactg
     3601 gcggcgcatc cggcgtgcgc tgggactgta caaagaagac agccatcctg agaacgtgtc
     3661 cgatgaatga gggattattt catcaggagc ccggctcttt ccgtgaccga aacaatcagg
     3721 tttatatggt tgacggccgc attgtgcggg gcatctcatc cacggcacgt gcgcattggg
     3781 aagcactgaa agagagttcc ttctacaaaa aacacagtgc tgccggtcgt attgtagcca
     3841 cccgcgagat tgcgccggac aacctgcctt cgccttttac tgaagagcag ctctgggact
     3901 gctacctcga acatgaccgc cttcctttca tcagctacgt ttatgagtgg tgcttcggca
     3961 tgctgaaaga tgcggcgctc ctgcatcttg acctgatgga aggagcactt cgtgaaggcc
     4021 ttattctgaa agatgcatcg gttttcaaca ttcagttttc cggtgcccgg gaagtattta
     4081 tcgatcttcc ttcttttgag cggtatgagg ccggaatgcc ctgggtgggc ttccgccagt
     4141 tctgccagca tttcctgtat cctcttatgc tgcaaagtta caaagacatt tcttttaaaa
     4201 actggtttaa aggaaatgtg gacggcatta ccccaaaaga atgcaataac attctttcgt
     4261 ttcgcgataa attccgtaaa ggcgtttttt ctctcgtata tctccagtca aagctggttg
     4321 attccatggg aaatgcaaag cgctccattg tttctgaagc acaggacagc gatttcggca
     4381 aagaaattat tcttgccaat gttaaaaaat tgcgcaaaat tatttgcggg ctggagtgga
     4441 agcctgcccg atctgaatgg tcggattaca cgaaaacaca cagttacgac gacacctgtt
     4501 ttgagcagaa atgccgcttt gtaagcgaag ctgttgcaga gaagcccctg aagctggcgt
     4561 gggacatagg ctgcaatacc gggcattttt cccggatact ggcgaaaaac agtgcatatg
     4621 ttgtagctct ggatattgat gagctgtccg tccagcggct gtacgagtcg cttcgtcagg
     4681 aagggacaga caatattctt cctctggtat ttgacctgac caatccctct cctccgcttg
     4741 gctggcgttg ccgggaacgg catgctttag agcaacgggg tgcgccggag ctggtcctgt
     4801 gtcttgcact ggtacatcat ctggtgatca caggcaatgt tccgctgcgg gaggttatcg
     4861 agtggctcgc ttctttccgc tgcgccattg ttatcgaaat gctgacaaaa gaagatgata
     4921 tggtcagaaa gcttcttctg aaccgggtgg atcaatatcc ggaatttacc attgacggtt
     4981 ttgaaagcct tgccgctccc tatttcaccg tgaagaaaaa agaagaggtc atggcaggaa
     5041 aacgtttcct ttatcattta ctcccacggg aataagcgtc tgcataacag acattcgttt
     5101 taatggcacc gccttcaggc agtgccattt ctttttctaa tgtatgcagc cgtttcgccg
     5161 attttacctt ccgttatctc ttttctgttt ttgtgtctct ttctgttaaa tttcttttga
     5221 gacgtttttt tctgtagaat gtagaaatcg cttatacaga aacaataccg gacaccaaca
     5281 aagtacagga acagaaccta tgaaatcctg ccgagctttt cgtacccttt ccgcagtttt
     5341 cgccttcatc gtcttgtgcg ttttttctgc cgcgggcaat acgctcacag atcagtcgct
     5401 ggcgcgcctt gcggcaaaac cgcctgtgtg cgatgacgtg gtggactttg tcgtctgtgc
     5461 cgacccgcag ccgggaggat tattaggcac accgcaagtt tttcttgata tgctggatga
     5521 atggaatgta ctgcagcctg accttgtcat gtgtgtgggc gatatgatca tgggcggtcc
     5581 ggcggatgtc gtcggttcca tgtgggatga atttctgggc aatgtgggta agcttcaggt
     5641 gccttttttt ccgacagccg ggaaccatga tgtcaatccg gagcctgctg tcatgagcac
     5701 ttatgaagaa cgggtatccc ctttttacta ttcggtcgta cggggcaaca gtgaatttat
     5761 tgtgctcaat acggaggagc cgggaaatcc tgacggcttt tctccgtctc agcggaagtg
     5821 gcttgaaaaa acgcttgtcg ccagcaaagc cagacacatc ttcatttttc tgcatgtccc
     5881 gctgtttcgc ggcaactggg cgcgggactg gcagcatatg gaagatcttt ttgtccaata
     5941 cccggtccgt gttgtttttg cgggacatga gcacctctat cgtgactggg gcacgcataa
     6001 aggaatccgt tatgtagtcg ccggcagtgc aggcggcggt ttccgcacgc cggaggaaga
     6061 aggcggtatg ttctgctatc tctgggtgaa agtccgggga gaagaagtat cctggtcggt
     6121 ggtccgcccc ggtgctgtgc ttccttccga tgttgtgact gaaaccaacg tgcagaaact
     6181 gcatgcgctg cagaaaattc ttgacattga agaaattgct gtacactgga acagcacact
     6241 ggatcagaaa atcgaagtac gcctgcacaa tcccttttcc gaagaaatca gcagtacttt
     6301 ccggtgggaa ataccggaag gctggacggt aacccctgcc tcccatacct ttaaagtcgc
     6361 cccgggaacc accgaagtgc agcagtgcca tgtgaaaaca gatggcgcct tccgttttcc
     6421 cagtcctgta ctgcagggaa aagtggaagc ggaagaactc gtgaggacgc tggaattaag
     6481 cccatcactg gatgtaaaac cgcaggcttc agcgccgcat gctccgggaa aggtcgtttt
     6541 ggatggtgac ctttctgaat ggtcgggggc tgttcccctt cccatgctat acggaagcag
     6601 ttatgacccg gcagagacgg aagatctgca ggcacaggcg cggctcatgt gggatgaaaa
     6661 caatttgtat atcgctgtgg aagtggaaga caacgaattt catcagcctc actacggtga
     6721 tgttgtgtgg atggccgata gcgttgagct gtgggtggaa gattcaaact ggagcttcag
     6781 cctcactgaa agcgggcctc aggtgtttct ggacgaacgc ccggacaagc atctggatgc
     6841 ggtcatcgac ggcgtaccgc tggctgtaaa acgggacgga aggcgtaccg tttatgaagc
     6901 ggcctacccc gcagaggagc tgcctcagat cgcctgccgt gccggaagtg tcatccgttt
     6961 ttccattctg gtcaacgatc tggatccttc gggtccactg acaaaacggc attatgcgga
     7021 actgacgccg ggtgccgggg ctcactttga ctgtgccaaa tattttataa cgctggaagc
     7081 agcccgctga tcctgttgtt cggaagcacg tcactgggtg gtcatcatat aggtgacctg
     7141 cacctttgcg gtacatccga tctgcgcgct ttctccatat tgcttttgag gctgctgctc
     7201 ccacacaacc tccttaatct ccactttttc aatggcataa atggtgcttt tcaaggcctc
     7261 tgaaactgct tctgcgtggg gaaaggcgtt ttcagttgcc cgtgccacca cttcggcttc
     7321 tatggcttct ttctgcatgg tgaagaagaa aggatcctcg agcgaagcct gcatctctga
     7381 ggcaaggagt gatagcctgt cggaaagtgc cgcaaaaaga gcggcgccgg agggcgtgtt
     7441 gaaggagctc atgctgaact gaaccagaat acccgatgaa accatacagc tggataccgg
     7501 cagaaccggg gtcatacgga tgtcagtggg ctgcagctcg gcatcccgga tctgagccag
     7561 ggcggcatgg cggtatgcat cacactgggc gaccgcttca ctcaagcttt ctccaccgaa
     7621 ttcccggttg aaaagaaatt ccacatgggt cggcaacgtc agttttgtcg ctgtaccgca
     7681 atttataatg accggagaat ccagtgcatg cgcctgaaca gaagcagtca ggaaaacagc
     7741 aacgaagctg caacgcagca gaaaatcagg caaagagcgc atatgcataa aatctttgtc
     7801 tccctaaatc ttcagcatcg gagcaaaaat atcttggagt ctttgcttgt ccggctgatt
     7861 tccgtattca cagacctgaa acatttcagc aaaacggcag gatgctttac cggcaggggt
     7921 ccactcagcc agtgcatcgc cgtgcagctt taccggcagt tccagttcgg gtgaaaagaa
     7981 ctgtttcaac cagaagaggc gttccgcagg atccgcatca gcggggggca cctctccggc
     8041 cttcccttcc agccagggca gccattcata aaactgtgaa tccatagcgt ccagcaattg
     8101 ccatttccgc ggcatcaggg tatccacagg cagcgcaacg tccgcgcgaa agggcaccgg
     8161 gtaggtaaag ggatcaaaga gataaagaaa aagcggattc ttctgaagcg cagggacatc
     8221 cgggcagaac tgaggaaccg tcaccataaa ggcagcatcc tgtaccgcct gcgccgcata
     8281 tctgtgatcc ggatgataat cattgggacg atgggtaaac acaatatccg cccggcatcg
     8341 gcggataata cgcacaatct ctttgcgcag ttccagtgta ggcatcagct cgccgtcatg
     8401 atgatccagc accagcgatt cataaccacc gatttctgct gcccgcgcag attcagccag
     8461 acgacgcgct gccagcatgc ccccacccag agcgtaatgt ccgatatcac cattggtaag
     8521 agaaacagcg agcacccggt gtcctgcttc agcaaagcga atcatggtcg cacctgcgaa
     8581 gacttcaggg tcatcgggat gggcgccgaa acacaccaga ttcatattac tgcaccgccc
     8641 gtttgaagtt gatgcggacg gggttgctca tgatggcgac ctgctcagca gaggtggctg
     8701 ttttcgggtc aagcagatct tccgccgtgc ctgtttccat gagccacgca tcggcccggg
     8761 catgtacaag catggtttcc ggaacagatt ccagccccgc catgacatct acggtgaacg
     8821 tgcgaggacg cggttttcca tcggcgccca cgggttcctg acgcgcatcc ctacccataa
     8881 tccaggcgct gctctcttcg gttattcgtt catcgtcacg ataggcatgg atacggacaa
     8941 caacaggcct ctcaacgaag ggtcgttggg aaaacaggct gaaactgata ggaagtgtct
     9001 cagggaaagg ctcttccaca gtgattgttg ccatggatag agaaccgcgg agtgtttcca
     9061 cataatcagc gctcacggtc gaggaggcag cgccccgtgc caattccgcc cgaatcgtca
     9121 gaccgctgtc cacatcgccc agatcaatcg gtggcttgga gggcatggtt gtgctctgaa
     9181 cagcggtgcc cgccgctacc ggtgctgccg cccgcgaagg gtctttttca gagtcacgtc
     9241 cgcagcccgc aaacatcaga acaaaggctg ttgcaaaaac cagaaacttt ctcataaact
     9301 tactccaggt acaaagggtt tcagacaagt cattttatca cggcacagtc aataaaatga
     9361 agatactgca aaatggtcct ttggggactg tgcgcaaaga gaaaaatata tggctctatc
     9421 ccgtcacatc ttctgcgttg cccttctttt caccctgcct ttttttctca tgacagccgc
     9481 agcggcttcc gatcaacctc aatggggaca gtgcttcagc cgcaatatgg tttcggaaga
     9541 agcaggactt cctgcgtcct ttgatcccca aacaggccac aatgtgctgt ggacagcccc
     9601 tctgggaggc ggttgttaca gcagcgctat tgttgcggaa ggctgcgtct tcatagggtg
     9661 caacaacagt gacccgaaag acccgcatca acaaggagat cgcggaacgc tctactgttt
     9721 gaatgaaaaa gacggatcgc tcaagtggca gctgctggtg ccccgcatcg ggggtgacga
     9781 ctttctcgac tggcccggca tcggcatatg ttcgccgccc accgttgaaa acggaagagt
     9841 ctacaccatg accaaccgga cggaagttgt gtgtcttgat cttgctggca tgacagacgg
     9901 caatggcggt gtatttcagg aagaagggcg acatatgact ccagccggct ctcctgctgt
     9961 tgaaccgggt gctgctgatg cggatattct ctggctttat gacctgcgca atgaactggg
    10021 cgtatatccg catgactctc cgcatacatc cattctgatt gacggtgact tcctttatct
    10081 gaacacctgc aacggaacaa ccaataccca tcgcgaaatc ggaaacccgg aagcacccgc
    10141 attcatcgta ctcaacaaac atacgggcag actggttgcg caggaacggg agggaatgag
    10201 ccgtcgcatg tttcattcca cctgggctcc gccttctctc ggagaagttc agggcaaacg
    10261 gctgattttc ttcggtggcc cggacggtat ctgctatgcc tttcataccc tcaaacaaca
    10321 ggtttatgag actgtacaag acctggaatt atgctggcgc ttcgactgtg atccgaccgc
    10381 tccgaaagaa aatgtgtttg attatttaaa caacaataag gaaggcccga gtcttatcat
    10441 gagtatgccg gtcttcatga atggccggat tcatgtcaca gcaggcgggg atatctggtg
    10501 gggcaagcgg cagtcctggc ttaaatgcgt cgacccggca gggacaggcg atgtaacggc
    10561 agccaaagaa atctggtcat atgaaattcc ctatcagtgc agcagcactc cggcagtgac
    10621 cgccgatctt gtttttgtgg gtgatgatgg cgggcagctt cattgtgtgg atgcaaaaac
    10681 aggcggagcc tgctgggttc acaagttcgg acggaagatc tggggctctg cgcttgtggc
    10741 agacgggaaa gtatttgctg cgggacgcaa cggcactctt gctgtttttg aaaattccag
    10801 agagaaaaaa ctgctttggg aaacacgctt tcccgaagaa atcaacggaa cgcctacggc
    10861 ggcaaacgga gtcctttatg tggcaacttt aagccgctta tacgccttga aggcgatgga
    10921 ctgacctaca ggtcgaagcg ctgcggttga gcttcccgca taattttcac accccgcgct
    10981 ttttgttctt ccaggcctga cttcagtgcc tgcaacgacg cattttcgcc atgaaccagc
    11041 aggatgtttt tcggccgcct cctgaaccgt gctccgaatt ccagcaattc ctgtcgcccg
    11101 gcgtgagcac tgaaaccgtt cagcgttttc acctccgcat tcaaggggcg ctttacgccg
    11161 aatatcttga tttccggctg cctttccaca atccgtcgac cgagggtgtt ctgcgcctgg
    11221 aacccaacaa taactatggt atttttagga ttttcgcaat gattgcggag gtgatgcagg
    11281 atgcgcccga cctcgcacat gcctgatgcg gaaatgataa tgcagggtcc cttaaaggag
    11341 ttgatgcgca gagaatcatc catgttgcgg atgtattcaa tgtgcttcag gagaaagggg
    11401 tcgccgcctt cctgcataac ctgtctgaat tcgtcatcaa aattttctgt atgatatctg
    11461 aatatttctg taatgttgac agtaaggggg ctgtccacaa aaacggggat ttccggaata
    11521 gtgcccgaca tttcgagacg cttgagacaa aataccacct cctgagcacg ttccaaagca
    11581 aaactaggta taatcacttt gccgccccgg ttgactgtgc gcaagaccac ttcagccagt
    11641 tttgcttcgt tggtttctat gggatcatga tcccggtcac cgtaagtgct ttccatcatc
    11701 acgacatcag catcgtccgg ctcccaggga tcctgcagca acggcatatt ctttctgccg
    11761 atatcgccgc tgaaaataaa acgccggtcc cggttctttt cccggtactg tacctccacc
    11821 atggcggaac ccagcacatg cccggcatcg tgaaaagtga cataaatatc aggggcaact
    11881 tcaaaacgtt cttcatagga gtaggacaca aaacgtttca tagtggcata cacatgattt
    11941 tcatcataga gcggcgcaat aaaactgcga tgttttttct gcagccattc agcgtctcgt
    12001 ttttgaataa aggcgctgtc taaaagcatg atgttgcata aattgtaggt ggcgtgggtg
    12061 gaatagatat gccctgtgta gccctgttta atcagcagcg gaagcacgcc gctgtgatcg
    12121 atatgcgcat ggcttaaaac aaccgcatcg agcgtggacg gatcaaaaag agattccctg
    12181 tttttccgat ccgcctgttc gcgggatccc tgaaaaagcc cgcagtcgag aagaatgcga
    12241 tgatcattta tttcaagaag gtgtttcgaa ccggtaacat tgcctgctgc accgaaagac
    12301 gtaattttca tagtacctcc tcctgttgtc cggtaaaggc agcaccttgt aaaaacggaa
    12361 agttcacaaa cggcgtgccc tgtccattat acgcctgaat ggcgcttgca ctgcaagaga
    12421 aaaaccagaa aagaacagag ccggctgatg tgtatattat ccctgccccc gcgcttcctt
    12481 tcccgcctgt tttttcaagg ctttgaatcc ggggaaagct cagagtaaaa tgagcaggac
    12541 agctgcccgt ttccctgcaa gctagaggaa tctgttatgt ccacaacacc gccagccggt
    12601 tccatacctg tgatttacgc gccaccgcct cagtccgatg gaacagtaca acaatatgtg
    12661 atggtacctg ttccggcgcc tcagtcaccg acaaaaagtt cgaagaaaga aagtgcgttc
    12721 gcctggctgg ttcttttcct gattcttaca gtgcatcttg cagcgaatct gtactggctg
    12781 cacacagacc ggcatctcat ccgcatggat gaagcccatc atatgaaaca atcgctggcg
    12841 taccatgacg ctttatttac agaggataca ggaagcactg cggaagggat cctggcggcg
    12901 ctttccatcg aatctcccta tccgcctctg ctgcatatta tcggtgccat ctgtatccgc
    12961 tacctgggag ggtctcccga ttcagcggct cttgccggaa gtatagcact ggcattgctc
    13021 ctgatcggcg ttttcgtttt aagccgtctg gtgcttgctt ctcaagcggc gctgctcgtt
    13081 gcagtcgtca ccggcttcac gcccatggtt ttcggttatg cgcgcctcat gataccggat
    13141 acttttatgg cggcgtggct ggtctggtgt ctagctgcgc tgatcagaac tggtgcattt
    13201 cataaaagcg gctggtcgct gctttgcggt ttctttgccg ggctggcgat gctgtcaaag
    13261 cagaacaccc tgatttacct gagtcccctg cttgcaggag cagttttgtg gggactcaaa
    13321 aaagcctttt cagaaaggaa acgcgacggg gcagataaat atactttttc acgcattctg
    13381 gtgaacctgc tcctcttcag ctgcaccttt gccgctgtgt gtggatggtg gtacattcgc
    13441 catgctgagt acctctatgg atggtggagc agcgcatccg aagaaaatgc aagtgttttt
    13501 caggaaccgg ctgcgagcct tttcgcaaaa gttcttcctc cttccggtgc ggttcctgct
    13561 ctggtaaccg atcctgatct gcctgttccg tataaagaaa tccccgcttc cgacaaaggg
    13621 agcggcatag cctttttctc agatgcttta taccgttatg gcacgatgaa tcttttttac
    13681 ttaattaaca gcgtcctttt tttgccgctg gcgcttgttg ccggattagg cgttctttcc
    13741 ctgctcctca aaaagaaccg aaacagcctt tcgctgattt tcctgctgtg ggttgcagcg
    13801 gtgtggttta ttctcacagg gctgtttgcg cttcaatcgc cccgcttcct gtacccgctt
    13861 ataccggcgc tgtctttttt tgtgtatctc gcacttaacg ggttatcggc cacccgcgct
    13921 ttcagagcgg cgtgggctct ggttttcatc gctcttctgc tccagttcat caacctcagt
    13981 tttgttcccg ccccgtacgc catgcatctc aaacttcctt tttttcaaaa cacggaggag
    14041 gcggaagaaa acgacgtgca accgttgact gttctgtcgg gtgaagtcgc cgcaggtaat
    14101 tacattattc aggcacctgt tacagaaata cctgtcagcg aaaaactcgt tgccgccatg
    14161 gaagcccact atccgcctga tgccaccgtg gcgtggtatc aaatcatcgg ggatatgccc
    14221 gacgctctcg gtgtggattt ttatgcacac agttttccgc accgctccct ttttgactgt
    14281 tttcttgaag cagacgcacc cgggaagcac cctgtttttt cagcgctgaa tgaagctgcg
    14341 gcagaactct cagcgcatcc tgccgatact gccgaagcag attttattat cctgcagcgt
    14401 gcaaaggcca gccagaccga gccgcttctt gaaaaagatg cagcacttct tatgaagcag
    14461 ggctttcaga tggcttttca cgctgttctg cccggagcgg aaaagcaaca gcctgcacaa
    14521 gtactgctta tggcccggac aactaccctt acacctggtg catgcaccga tcttttttcg
    14581 ctttatgacc tgctggagcg ggacgggcgt gaatgggtgc tcaccgatga agaaaggcgg
    14641 gatgcggaat ctcgctatgc ccggcagata tcggcctatg ctgtgccccg ccccttaaaa
    14701 cagggtattc attttatggg ggttcatatt gcgcaagcgg ccgaaaatgc ctatctgatc
    14761 agaattatcc tgcatgttca gcagacaccg caggaaaaac tgcgtgtgtg gatgcggggc
    14821 atatccgaaa cagcgggagc tcaggcagcc atttccgaag cggcatcctg ggacttcgat
    14881 ccggatccgc cttccatgca atggggcgaa aatgaggcaa tcattctttc cagaccggtg
    14941 ctggtttccc cggcggtttt ccatctggaa atcgggctat tcaacccgga aacagcggac
    15001 agacccctag cccttctgga aacggagaca gtcgatttcg cagaaacaga ccgactggac
    15061 agggactaca gggaggcgtt ataagggagc ctgattcagt cccggcggct cggagcccag
    15121 attgtagact ttgaccggaa ccagtccagc cgttggatgg agcgctttca tggcgcttct
    15181 ggagaggtca atgacacggt ttttcacaaa aggaccgcgg tcattgatcc ggcacacgac
    15241 agatttgccg ttttctgcca ctacaagtac atagtctcca aattcgcctg tgcgcatggc
    15301 gcaggtcatc gcgttgtcct tcaaacgttc gccggatgcg gtgatgcctc cggtgcctct
    15361 taccgtgtag taagatgcca gcccttcaac tacgggctgc atgggctgat gtgacaacaa
    15421 cacgaaagca ataacggtaa acatatacct tgctcgactc cgcctgactg attcttctgt
    15481 gcacccgcga cccaagccac agttgtccat atgcaatgta caacaatgat tcaggctacc
    15541 ccttcaatgc actaaatttc agcccggctg ttttgtgtga tcccggcgca gcccaactgc
    15601 gcagaccatg atctaataac aaacatgagt ggtatcacac attcgttcag caaatacagt
    15661 tcaagccatt atacaaaacg cgacacttaa agtcaacttt tagggaaacg ccgtgtcgta
    15721 actctttaga aatgtcccct tccgtaactc ttttaaactg tccccttgtt ttctgagtaa
    15781 tgcatttttg gtgcactccg ccgacccgat acgcactgcc acgcaaaaga aaattatacc
    15841 gccattgggc ttgtaagttt gcgtacgcag tgctacactg taaccagaat tgaacaaagc
    15901 cgtttctgtc ctttcatatt tgtactatgg gtcagtgtct ttcatactta cttcaacagg
    15961 cgaaaggaag tctgtcatgt gcgctcagga acatcacgac cattcttctg cacattccca
    16021 ccgttgttcg ggctgctgcg gcacccggtt ctcggaattg agccgtcgcc agttcattgc
    16081 aggcacaggg gcggtcgcgc tgggcagtgt tttcatgcac cacctgcagg ggcgcaccgc
    16141 ctttgctgac acggtgccgc gccgcatcct gaaacaggac agacctctgc gtattcagcc
    16201 cgtactcacc tgctccctgc cccgatattc cgaagcaacc tcgtggcgcc cctggggtgg
    16261 tctgctcacc gattcacatg tgcgggagga atgcgcaaaa atccaggacg aactgaacgc
    16321 catggctgcc aaagcagatt ttccattgga acttctgccg ttgaagacag tacagtccgc
    16381 tgaagaagga cgctcgatag ccgcggaaga tcacgacggg acactgatct ttggtgcagc
    16441 cggcggcacc gatgttatta tttcgcttgc cgatcctaaa aaacacaacc tgctttttgt
    16501 ccggcataaa tcaggacccg tctatctttg gtttgaaata gccgacccgc ggtttctgcg
    16561 tcaggaaagt gactttccca aaggtacggg cggcatggat cacaatgatg tcattgttga
    16621 cgattttgat gaaatcctgt ggcggttgag agcactccac ggtctgaaga atatccgcaa
    16681 taaacgcatt gttgtcatcg gcaacgccat cggctgggac cggggtacag ccagtgattc
    16741 ccctaaacgc gctcaggagc tctggggact ggattatcgg gagattccct atacagaaat
    16801 gggagagcgc attcgtgccg cccgtgccaa tgatgctttg gtgcggcagt gccgtgatat
    16861 gacagaagcc tatctgcgac agcccggagt aactcttttg ccccgccagc agcagctgac
    16921 cacccgggaa ctgatcgccg gtgccggttc gtcggatact cttcaggaga tacgtgtctt
    16981 tacggaaaaa gcgttcctgc tgactgaggt attccgcgat cttatggaag aacatgagac
    17041 agatgccctt accgtaagcg aatgtatggg tacgattatc agcatgtctg aaacaactgc
    17101 ctgcctctgc ctgacgctgc tgaacgatga aggctgcatg gccttttgcg aatctgattt
    17161 cgccgtgatt ccctcaggaa tcctgctgca ttacatctgc tgcaaacctg tattcctctg
    17221 taatcccact ttcccgcata acaacatgat tactgtggcg cactgtacgg cgccacgaaa
    17281 aatggatggt gtgaatgcgg aaaatatgtt cgtacgcacc cactttgaat ctgactatgg
    17341 cgctgctccg aaaatcaatt tcaaactggg acagaatatg accattctcg ctcctgattt
    17401 cgcaagcagg cgctggatgg gctttgaagg aatggttctg gaaaatcccg aactggatat
    17461 ctgcacgagc caggtagacc tgcgtattaa cggcgactgc gaccgcttgc tgcatgaaaa
    17521 agcaggattc catgtcatgg cgtgctacga caattatctt cgtgaagtcg ggtatgcact
    17581 gcataaggcc ggcgtggagt gggtaaatct ttccgcatag cgatattcct ttcagaacga
    17641 atgctgcgac aggagattgc ctccatcccg tccccctgcg gttacgggca ttttgtccat
    17701 gccgaaatga agggaatgag catctgccgg acaggcacgc tcacggctcc gattgatgtc
    17761 tctttctctt tcatggataa taggacaggc ttggagttat gtcggatctg gtcactctga
    17821 aggaatcaga cggcgctgtc aaaacataaa gggtgtatcg tgaaaacagt ctatgtggtc
    17881 acaatgtggt ctggcggtcg tcccgcaaag aaatggaaaa cagagcacgc tccggaactt
    17941 cttgcgcagg ggaccggcat ttctttcatt aatgcgggca ctaaaatgca ggtgtctgtt
    18001 atcggcagta tttctgttga agaatacgaa tccggcaagg aagagcttga aatgggtctg
    18061 aatccgaccc cgggcattac atctgatttt tcgcagggga aaccggacaa ctgtgaatct
    18121 ctttccgatt ttaagcgcag caaaaaaaac atatttaccg atgaagttca ggaataacgg
    18181 aaaagacata ccccctgacc tcaaagggat gcacctgttg aatggcggat aaactatcaa
    18241 accagcactt tgagtcccgc ccgcggcagc tcattcaaag gcactggctc ctttttttct
    18301 gcggagaggc atcaggaatt ctctgggctc ttttcctggt agacacagca gcccttacca
    18361 ttgctgtcgt gtttctcctt tgctgtgccc tgtactttta tccgggactc cttccggggc
    18421 gccgtttggt catctggttt ctcctctttt ttgccttcgg aaatcttctg gttgtttttc
    18481 acggccttcc gcgcaaagcg gacaaactgc atgagctggc gctctcttat ccgcgcggca
    18541 tgatggagtt tgaaggaacg gcatccgata caagcccctt tctgtccggt cgcactgcat
    18601 acagcacttg tgttcttcat gtggacacgc tggtgaaaga caaagcatcc atgccgttat
    18661 ccggaaaagc agtgctccgg tggagcgaac cttcctgcgc gcttttcaac ggatcccgtg
    18721 tccgtgttat cggccgcctc tctccgcatc tgagcaccgt gaactacggc gtgacagaca
    18781 gcgaagaccg tcggcgccgg cagggtgttt tttccggtat ccatacccgg aacgggcagg
    18841 tacaaatcat tcatgatccg ggctttactc ttgccggtat ggcatcacgt ctccggcaaa
    18901 gtcaggcgga gcatctcgta aaaacagtac cggaagaagt atttcccttt gttctgggca
    18961 tatggcttgg cgatcgctcc cttatcaccg atgagaccta tcagatcttt gcatggtcgg
    19021 ggacagccca cgttctggct gtctcggggc tgcatgtggg tgttattgcg ctcacttttc
    19081 cctttttatg cagactcctt cgcctccgga aacgatacac acttctgtgt tccatggcag
    19141 gcattcttct tttcacattg atgaccggag cacatatcag cgtcatccgg gcctctctga
    19201 tgatgcttct ttattatttg tatcagctgc tcagaagaga gccagacacc atttcggttc
    19261 tcagcctgtc cggagtcatt tttttaacca ttcagcctca tgtgattttg gatattgcct
    19321 ttctccttag tttcggaagt gtgtcctccc ttcttctctt ttatccgggc atcctcgcac
    19381 gcctgcagaa tctgccccgt tttttaagtt ccgcctgcgc cgtcacgctg gcatcacaag
    19441 taatcacagt gcctattatc gcatggtcat tttttactgt gtctctgacc ggt
//
LOCUS       DAOPWB010000056        18801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_2981_length_18801_cov_5.332017, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000056 DAOPWB010000000
VERSION     DAOPWB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 18801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18801
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2981_length_18801_cov_5.332017"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKN92_06900"
     CDS             complement(<1..396)
                     /locus_tag="PKN92_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ48556.1"
                     /translation="MPETTCSRREFLATGTTVLAGTALLGHISTAVASSSGTSMIAAN
                     SSEIRLLQFTDLHFFAGISHEPEISEIQRQQTFDDMRRLVDLTRPDLLAVTGDFWHEN
                     PDGRGLEFLHFSVEKVADLGIPWVFTWGNH"
     gene            complement(469..1491)
                     /locus_tag="PKN92_06905"
     CDS             complement(469..1491)
                     /locus_tag="PKN92_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ48557.1"
                     /translation="MKHPSFSRRQFMGASAAAAGSGFFFHGTGIAAAADGFPGERILT
                     VPEKDQLRLLQFTDLHFFNAINKYPVREEYLRLKTIGIMRRLVDHARPDLIIATGDLW
                     HENTDGRGMEFMHWAADQIAALGVPWTLTWGNHDDVDDQAAAHRFLTTALHSVYDGEA
                     NQGHHVVAVEMSGGKRILELFCLNSGDTGIKADALDFLSRYAEAYTYADGEAPLRMSA
                     FHIPLKQYQDRWDDGTARGLLGEKVYFESEDGSALSGFVKAGISAIFCGHDHESDYSA
                     FSDGIELIYGRATGYNGYGAETLDKGAKLYEIDTRTRTYRWKTLFPDGTTWQPGKDEH
                     LDISEE"
     gene            complement(1558..4158)
                     /locus_tag="PKN92_06910"
     CDS             complement(1558..4158)
                     /locus_tag="PKN92_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48558.1"
                     /translation="MRTSIQVILSVAFVTAVFFSAAMSSAAEESSFADDLKEAASYAF
                     GQSDAALRRIESRLYHGAAAGEEQAALLAEYEAQILSLLQAECSPEGRSFLCRFLADI
                     GGKASIPVLGSMLSDADQFQLAVSALEKITDDAAARTLAERLADASEKDRITLLEALG
                     RQKAASSAAAVRPYLAGSAQESLAAVNTLAAIGTTESCGLAAAAIKNATPEKQILWAD
                     AGLKCAEQLGSTGDFSLLEIYKGTAFPVQVRIAALHSLIRMQPDKAEDYVVEGLQDGD
                     ADLASDSLSLARTVKGEKITDCLIALLDAAPDTQKPAYIEVLGVRGGQKVLDKAVTFA
                     GYAHLNTRLAALKTVGALGDASFVPFFLQQITAGGAEEQRTAKEMLTVMQDGQTDRTL
                     LDTAVSGEDVKLRAAAIEMLSERRAVDTADTLCALAYDAPAEVRSEAVHALRILGKPS
                     MTGQMLFLTLIPGLSEVKPVLPQTIAALIQRGSSVAETQSAAEKDAPVTRFYNELKEL
                     QKKGELPADTLRGAVCLVLDTFALLGDDLSFTHVQEALDDSDTEVRKAALGAITKFQR
                     ADALDALQRRITEEKEDGLRSLAYSSYLSVLKNATVLPGRVIDAHLDYAFEHAKNVSE
                     RREFLAAVAKTPSIRALQLAESLLKDPETAGEATRATATLAIALCGAWPQEAAVHLNA
                     IAASDAPAALKTNVEKARALMAHFGNYLMAWEYAGPYFEENLMATHLYEKELLPQKDA
                     EKAPWKTLPMLIDSPLPVALEFDRIWGGEERVVFVRTLLKTATDQDLILAVGSNDGCR
                     IWLNGKEIFAIADGRPLVPDENKIPVHLSAGENRLMMAVYQQGGAWRATARLTDLSGA
                     PAQGVEAKVQ"
     gene            complement(4216..5526)
                     /locus_tag="PKN92_06915"
     CDS             complement(4216..5526)
                     /locus_tag="PKN92_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48559.1"
                     /translation="MASKHNSLSRRSFMKQAAAAIALPAVIPASALGRDGHTPPSERI
                     VMASIGVGGQGMYDTRGLMSIGDVQYVALCDVDRKHCANAKKEVEAFYQGKKNSDSFK
                     GITAYHDFREVLARDDIDAVMIATPDHWHALISIAAAQAGKDIYCEKPLANSIPEGRA
                     VVNAVRQYQRVFQTGSHERSRDNSRYACELVRNGRIGKLHTIRVQMPVDHDYGIIYPE
                     MPVPEHFDYDFWLGPAREAPYTEKRCHFYFRYILDYSAGEMSDRGAHILDLGQLGNGT
                     DDTTPISVEGTGEFPKQGLFNTAYAYNFAFEYANGVRLVGDNRGPRGIRFEGDEGWVF
                     IHVHAGTLEAEPQSLLREVIGPNEIHLGRSRGHHNDFIECVKSRGEPKAPVEVGHHTA
                     VMCHMANIAMRLGKKLKWDPATERFDDDTANRMMQPSMRAPWTL"
     gene            5691..6284
                     /locus_tag="PKN92_06920"
     CDS             5691..6284
                     /locus_tag="PKN92_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNZ48560.1"
                     /translation="MKLQEPGKVHFLLYLLASLLLKLLGWKVENTPPAAPKYVVIVAP
                     HTSNWDYPIALLISWKYRMKGAWLGKEEIFRWPVLRWLFRGTGGIPVDRKHHNNLVGQ
                     AVHEFNTRDALIFALAPEGTRARQGYWRSGFYHIACRAQVPVALAYLDYRRKAGGFGP
                     LYECTGDVQKDLAYISDFYETITPRHPEKRTPVCFRQ"
     gene            complement(6371..8893)
                     /gene="leuS"
                     /locus_tag="PKN92_06925"
     CDS             complement(6371..8893)
                     /gene="leuS"
                     /locus_tag="PKN92_06925"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNZ48561.1"
                     /translation="MTAAYEPQAIELKWQKYWLENKTFKSVIDHSKPKYYVLDMFPYP
                     SGDGLHVGHPEGYTATDIIARYKRMRGFNVLHPMGWDAFGLPAERHAVRTGEHPAIIT
                     NSNCDTFRRQIQRLGLSYDWDREINTTDPKYYKWTQWIFSILYERGLAYEIEAPVNWC
                     PALNTVLANEEVKDGRYVETGDPVEKRPLRQWMLKITEYAERLLADLDLLDWPEGIKA
                     MQREWIGRSEGADVNFTVAGSGQVFTVFTTRPDTLFGATYCVLAPEHPLTLEITTSEQ
                     MDAVKAYVEAASNKSAQDRMKEEKEKTGVFTGSFAVNPVNGALVPIWTADYVLAEYGY
                     GAIMAVPAHDTRDYEFAKRFDIPVIEVISGGNIEKEAWTGDGVLVNSPLINGLHVDEA
                     KKTITAWLEEKGLGKGTVNYKLRDWLFSRQRYWGEPFPLIRLQDGTIKTLPMKDLPLL
                     LPELDDYRPTADGAPPLARAAAWVNTTDPETGQPAIRETNTMPQWAGSCWYFLRFCDP
                     TNENEAWSEEAERYWMPVDLYVGGAEHAVLHLLYSRFWHKVLYDAGYVHTKEPATKLF
                     NQGMILAYSFKDSNGKYHYPHEVERDGDHWRLKATGEMVETQIEKMSKSRYNVVNPDD
                     VVKSYGADAMRLYEMFMGPLDRDKPWTDEGVQGVYRFLRRIWSAFVNEDGKLHPRIQD
                     QASDDALLRELHKTIKAVTDGIESLSFNTAIARMMEFVNALLKTASFDRKSAESFLLL
                     LAPFAPHLAEELWEKMGKDTTLAYENWPSFDPALLVEDNIEIPVQVLGKLRGRIMVPA
                     SAPEADILTAAKTDPKIAPHLEGKAVVKEIYVPGKMVNLIVK"
     gene            complement(8890..10173)
                     /locus_tag="PKN92_06930"
     CDS             complement(8890..10173)
                     /locus_tag="PKN92_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ48562.1"
                     /translation="MKTITVEVREDHLETLAQTKPMAALSELIWNALDAEATEVRVAF
                     IENELTGLETIRISDNGHGLHYDDALLVFRNLGGSWKRHGSRTHERKRVLHGKFGKGR
                     FRAFSLGNSVCWHSVYKDGAAQASFKLSGHAAKLGEFTVFDRKGQVQQPSGMTVEIQD
                     VRDTSNLLRGVKAMEEVTNVFALYLRQYPDTHIIYDGIPLDPSNAETRFTTYPLGEMV
                     TEDGKRIEATLDVVEWRLPGKRALLLCDENGFMRIQAQPRIYFRGFSYSAYLKSAHIA
                     ALDSEGFLETGELHPDIRQLLNASRLKLREHFALREAESAIEVLDYWKETGLYPYSGT
                     PADNQEANERRIFDIYSTHLERIFSDFSGATLRTKRLVLRLMQELVHSDPVRMAGILD
                     ELITFPEEMEEQILELAGAADADETESRQRETSQE"
     gene            10460..12118
                     /locus_tag="PKN92_06935"
     CDS             10460..12118
                     /locus_tag="PKN92_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNZ48563.1"
                     /translation="MKQIIIEQPGVAPVTAVLSTGEIRLGRAENNDITLTAEEVSRHH
                     ATFAIVDGQCVVTDLKSLNGTYINRQRIATGIARHMDEVWLGSKCRIVYRDDETQPVP
                     VASSQEIKERLAQSIRHIRSEMQQVDKHVAQMKEQQQKTDTTLMGDASAFTMSPGEVA
                     RMSHAYRRLDALFRAGQAVASNFDLKTRLTGMLKIMMTTLNACRGFVLLRNEKNHTLQ
                     VEVSLQMGTELNASAPSMGIAGRAAFDGEPVLMKDRQDCMEFGGRQSIIANKITSAMC
                     VPLRGKEQLLGSIYVDTDQDNVNFNEEDLELFLSLSAQAALAIENVRLHEQTVEFERK
                     RGELSRFLPGPLVEKLMNQSEALTLGGNKTRVTTLFCDIRGSTHIAEQLSPQELVTLL
                     NEHFTAMTEIVFAYGGTLDKYIGDEIMAIFGTPLPTGDEEFRAVCAAVEMQLCNVRLN
                     AVRQSEGRFPLQIGIGIDVGVVIAGFIGSPKRMDFTVIGDKVNTAKRFCDMAGPGKVV
                     VGEDVWQKIHDHAQAKPLGAVALKGKQHPVQSYEILTVKRPNQA"
     gene            12160..13698
                     /locus_tag="PKN92_06940"
     CDS             12160..13698
                     /locus_tag="PKN92_06940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ48564.1"
                     /translation="MTEHIKHPAWLTGLFSREALPGWVIFFLALFVRAVHQWFIQQND
                     PMFGYLLTGGDNHTFDRWALEISQTFWLGWDRTPFFHGPLYPYVLGLFYLRFGYSPLT
                     AAWMQHGIGSLSAVLVFYLARKAFNQKTGWIAGIIAVFCPIFLLYECEILSDTLILFT
                     NLLLLWAAFHAAESQTKKAWFLLGLATGVAALGRPNALILIPVIALWLWVSVKKTWKT
                     KTSAAVLFTFTAVACILPATCANYFAGKHFALVSWSGPFNLYIGNAPDGTGVFWSPPS
                     MQKIRAEEKKPDLEINWSRYMRQALLDDPLLLPRSLWLKFRLFWQSGEIPHNINVYLK
                     KPFSPFLRIPLYWGMIAPLGILGFFWTVLRKKTFSWTDPVLILGFFFAVYMAGIVLVF
                     VLARLRMPALAVLTIFAAGALSQMHDLCREARVKRSMRPVAMILLLLLLWSCGGVLLK
                     SRDDTLLLRWNDYFNLGSAYESKNRRQEALEQYEKAVERAPEIIAIQGVRDEMRRQLE
                     QRQQ"
     gene            13926..14942
                     /gene="aroF"
                     /locus_tag="PKN92_06945"
     CDS             13926..14942
                     /gene="aroF"
                     /locus_tag="PKN92_06945"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNZ48565.1"
                     /translation="MIIVMSTSRQEDVDHVVARVEEFGYKPHVIYGVERTVVAAVGDE
                     RGKERLTSLESLPYVEQVIRILKPFKLAGREVKQENTVIECAGVLIGGNHFALMAGPC
                     SVESEEQIMESARIAKEAGANILRGGAFKPRTSPYSFQGMEMEGLRALRKAADAYHMP
                     FVTEATTPEQIPVVAQYADMIQIGARNMQNYGLLRAAGKAGKPVLLKRGMMSTINEFL
                     MSAEYILSEGNPHVILCERGIRTFETETRNTLDIQAVPVVKLHSHLPVIVDPSHAAGR
                     WDIIIPMACAAVAAGADGIIVEMHPNPEVALSDGQQSLRPKKFQRLVETLKPLLAVMK
                     KEIS"
     gene            14962..15543
                     /gene="scpB"
                     /locus_tag="PKN92_06950"
     CDS             14962..15543
                     /gene="scpB"
                     /locus_tag="PKN92_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNZ48566.1"
                     /translation="MSQEPLEKDEFPEIVESLGREESRRTLYALLFVSDRPVGVKRLA
                     EALGDMDEEMAGVLLNELMQELNSREELPYSLQQIAGGYQLITKTEYAPYIRRFFQIK
                     KSNRMSKALLETLAVIAYKQPVTRAEVEAIRGVSVSYAFDQLQEKRFIRISGVADLPG
                     RPKLYRTTEEFLMAFGLQSLKELPSLDELKIMD"
     gene            15546..16286
                     /locus_tag="PKN92_06955"
     CDS             15546..16286
                     /locus_tag="PKN92_06955"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ48567.1"
                     /translation="MALIRLQKYLAEKGLGSRRACERMILEGKVAVDGSVAEVGQQIE
                     PERQKITVNGMPLEQDEAEKVYIVLNKPAHVITTVKDTHGRRTVMDCLQGLDERVFPV
                     GRLDQDVSGVLLFTNDGDLAYQLAHPKFLVHKTYHAWVKGHISEEALQWLREGVSLED
                     GFTAPAKASLLKNKKGVSLVELTIHEGRKREVKRMFKAVGYPVEKLHRTTFADIKATG
                     LRPGEWRRLSDLEIKALRQCVAACSEKQ"
     gene            16289..16753
                     /locus_tag="PKN92_06960"
     CDS             16289..16753
                     /locus_tag="PKN92_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNZ48568.1"
                     /translation="MDHFDSTCITRLCNALHSIEEDALPRWGTLTKSAMINHFIWMIR
                     HSMGRSFLVPDYSTFLSRTLSKKLVLSGVRALPKNRCLPAHLCKQGISDQETGDLETL
                     QALMEEYLDLVQTGDLEPAPHPRYGRLTIDEWDRLHMLHFEHHLQQFNRSMR"
     gene            17044..17517
                     /locus_tag="PKN92_06965"
     CDS             17044..17517
                     /locus_tag="PKN92_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNZ48569.1"
                     /translation="MSTATKDLSYIEELVAQYPEHKGGTLTIMQKIQEREGCLSPEAL
                     DELARCSGVSVNELYGVATFYSFYKFSPPGKHTIQVCLGTACHVRGGAKILAELERLL
                     GIKVGEVTADGKFGLVRIACLGCCALEPVIAIDGKFYPRVSVRQLPSILAAYQDQ"
     gene            17535..>18801
                     /locus_tag="PKN92_06970"
     CDS             17535..>18801
                     /locus_tag="PKN92_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNZ48570.1"
                     /translation="MTFLNTRYEAARKEWQDLRAAAHPVIYVGAATCGRAAGAGAVID
                     YLCDAVTVGQLTAQIIEVGCLGPCYLEPLVIVQKPGMPRICYGNIGIAEMEGLVARFL
                     KGDDVCRQWALGTLDDAEVDGIAPLSEHPVMARQVRRIFSNCGIIDPENFNHYLARGG
                     YRGFLHALEIGREKTLDEVRRSGLRGRGGAGFPTFKKWEFCKNSPGEKRFLICNCSEG
                     DPGSFMNRSLIEGDPHAVLEGMLIAGYAMNATAGYVYCGVEYATVFNRLNTAIDQMRA
                     HGLLGEAVGGSDFSFDIIVKRGAGAYVCGEETALIAAIESRRGMPRPRPPFPAASGLW
                     GYPTNIQNVETLGNLPLILRNGADWYTECGTENNKGTRSFSMAGSIRHPGLIEVPLGM
                     TLKDIIYDVGAVRLWGVPLKPCRRADPPAD"
BASE COUNT         4427 a         4749 c         5058 g         4567 t
ORIGIN      
        1 atgatttccc cacgtaaaaa cccaggggat ccctaagtcc gctacttttt ccacagaaaa
       61 atgaagaaat tccagacccc ttccgtccgg attttcatgc cagaagtcac ctgtgacagc
      121 cagaaggtcc ggccgggtaa ggtctacgag gcggcgcata tcgtcgaaag tctgttgcct
      181 ttgtatttcg gaaatttcag gttcatggct gatccctgcg aaaaaatgca gatcagtaaa
      241 ttggaggagc cggatttccg agctgttggc tgcaatcatg gacgttcctg aagaggaagc
      301 cactgctgta gaaatgtgtc caagcaaggc tgtaccggca agcaccgtcg tgccggtcgc
      361 cagaaattct cttcgtgaac aagtcgtttc aggcatggtt atctctttgt tttgtggaaa
      421 aattgtgctt tatccgggca ggttgtgaga ggcagaggat gccgccgttc actcttcgct
      481 gatgtccaga tgttcatctt ttcccggctg ccaggttgtg ccgtccggaa acagggtttt
      541 ccagcggtag gtgcgggtgc gggtatcaat ttcgtacaac tttgcaccct tgtccagcgt
      601 ttctgcccca tagccgttgt acccggtggc tcgtccgtaa atcagttcta tgccgtccga
      661 gaaggcagag tagtcacttt catgatcatg accgcagaag atggcagaga taccggcttt
      721 gacgaatccg gaaagagcac ttccatcttc cgattcaaaa tagacttttt cacccagaag
      781 tccccgggca gtgccgtcgt cccatcgatc ctgatactgc ttcaggggaa tatgaaatgc
      841 gctcatgcgc agcggtgctt ctccgtctgc ataggtatac gcttccgcat agcggcttag
      901 aaaatccaat gcatccgctt taatgccggt gtccccgctg ttcagacaga acagttcaag
      961 aatacgtttt ccgccggaca tttctacagc gacgacatgg tgcccctgat tggcttcgcc
     1021 atcataaact gaatgaagag ctgtagtgag aaaacggtgc gcagccgctt ggtcatccac
     1081 atcatcatga ttaccccagg tcaaagtcca gggtacgccc agtgccgcaa tctgatcagc
     1141 agcccagtgc atgaattcca taccgcgccc gtccgtattc tcgtgccaca gatctccggt
     1201 tgcgataatc agatcgggcc gggcatgatc caccagacgc ctcataatcc caattgtttt
     1261 taaacgaaga tattcctccc gcacgggata tttgttgata gcgttaaaga aatggagatc
     1321 agtaaactgc aggagacgga gctgatcttt ttcaggtacg gtgagaatac gttcgcccgg
     1381 aaacccgtca gcagcagcgg caatacccgt cccgtgaaaa aagaacccgg atccggcagc
     1441 tgctgcactg gctcccataa actgacgtcg tgaaaaggac gggtgtttca tcgttatttc
     1501 ctgtttcggc gcagcaattg ccgcagcggt agtttgtgtt ttttagagca tcacacgtta
     1561 ctggactttt gcttcaacgc cttgtgccgg ggcgcctgaa aggtcagtaa ggcgcgctgt
     1621 tgcacgccag gcaccgccct gctggtagac cgccatcatc agcctgtttt ctcctgctga
     1681 cagatggaca ggtattttgt tttcatcagg aacaagaggc cgcccatcgg caatggcaaa
     1741 aatttccttt ccattcagcc atatcctgca cccgtcatta ctgcccaccg ccagaatcag
     1801 atcctgatct gttgcggttt taagaagcgt tctaacgaac acgactctct cttcaccgcc
     1861 ccagatacgg tcgaattcca gcgccacagg cagtggagaa tcgatgagca tgggcagcgt
     1921 tttccagggt gccttttcgg catctttttg gggaagaagt tccttttcat acagatgggt
     1981 ggccatgaga ttttcttcaa agtaaggacc ggcgtattcc caggccatca gataattgcc
     2041 gaaatgagcc atgagtgcgc gggctttttc aacatttgtt ttaagcgcag ccggggcatc
     2101 cgatgcagcg atggcattca ggtgaacagc agcttcctga ggccaggcac cgcagagcgc
     2161 tattgccaat gtggcggtgg cgcgggttgc ttcacctgcc gtttcaggat ctttcagaag
     2221 actttctgcc agttgcagcg cacggataga gggcgtcttt gctacggcag caagaaattc
     2281 cctgcgttca gaaacgtttt tcgcatgctc aaaagcataa tccagatgcg catctataac
     2341 acgacccggc agcaccgtcg catttttcaa tacactcagg tagcttgaat aagcaagaga
     2401 gcgcagcccg tcttcttttt cttcagtgat ccggcgctgc aatgcgtcga gcgcatcagc
     2461 acgctggaat ttagtgatgg cgcccagtgc cgccttccgc acttctgtgt cgctgtcgtc
     2521 cagcgcttcc tgcacatggg taaaggatag gtcatctcca agcaatgcaa aagtatccag
     2581 aacaaggcat acggcaccgc gaagggtgtc tgcagggagt tcgccttttt tctgcagttc
     2641 tttcaattcg ttataaaaac gggtaacagg cgcatccttt tcagcagcac tctgcgtttc
     2701 agcgaccgat gaaccacgct gaatgagtgc ggctatcgtt tgcggaagta caggtttcac
     2761 ttctgaaagc ccgggaatca aagtcaggaa aagcatttgt cccgtcattg agggctttcc
     2821 aagaatgcgc agagcatgaa cggcttcgct gcgtacttct gccggagcat cataggcgag
     2881 ggcacacagg gtgtcggctg tgtctacagc acgccgttct gaaagcattt caatggctgc
     2941 cgcacgaagt ttaacatctt ctcccgaaac tgccgtgtca agcagtgtcc gatccgtctg
     3001 accatcttgc atgactgtca gcatttcttt ggcagtgcgc tgctcttccg cgcctcctgc
     3061 tgtaatttgc tggaggaaaa aaggaacaaa agaggcatct ccgagtgcgc ccacagtttt
     3121 taaagcggcg aggcgggtgt tgaggtgcgc atagcctgca aaagttacgg ctttatcaag
     3181 cactttctgt ccgccccgca caccaagcac ttcaatatat gcgggctttt gcgtgtccgg
     3241 tgccgcatca agcagagcga tcaggcagtc cgtgattttt tcgcccttta ctgtgcgtgc
     3301 cagagaaagg ctgtccgatg ccagatcggc atctccatcc tgaagtccct ctactacata
     3361 atcttctgct ttgtccggct gcatgcgaat cagactgtgc aatgcggcaa tgcgcacctg
     3421 gacgggaaag gctgttccct tatatatttc cagcagggaa aaatcaccgg tactgcccag
     3481 ctgttcagca catttgagac cggcgtcagc ccagagaatc tgtttttccg gagtggcatt
     3541 ttttatggca gcagctgcta atccgcagct ttccgttgtg cctatagctg ccaatgtatt
     3601 gaccgcagcc agactttcct gtgcggaacc tgccagatac ggccgtacag ccgccgccga
     3661 ggatgccgct ttctgccgcc ccaatgcttc caacagagtg atacggtctt tttcgctggc
     3721 atcagcaagg cgttctgcca gagttcgggc tgcagcgtca tcggtgattt tttccagagc
     3781 agatacggcc aactggaact gatcggcatc ggaaagcatg gagcccagca cagggatgga
     3841 ggcttttccg ccaatatcgg caaggaaacg acagaggaag ctacggcctt ccgggctgca
     3901 ttctgcctga agcagagaga gaatttgcgc ttcatattca gcaagaagag ctgcctgttc
     3961 ttcaccggct gcggcaccat ggtaaagccg gctttcaatg cgccgaagag cggcgtcgga
     4021 ttgtccgaaa gcataagagg ctgcttcttt cagatcatca gcaaaggaag attcttccgc
     4081 cgcactgctc attgccgctg aaaaaaatac agcggtaaca aaggcaacgc tgaggatgac
     4141 ttggatagag gttctcataa gatgtatcct ttcccgcagt gaaatgccca acgggttgtt
     4201 ttactgaaat aagggttaga gggtccaggg agcgcgcatg gaaggctgca tcatgcggtt
     4261 cgccgtgtca tcatcgaagc gctccgttgc aggatcccat ttcagttttt tgccgaggcg
     4321 catggcgata ttcgccatgt ggcacatgac tgccgtatga tggcccacct ctaccggcgc
     4381 tttcggttca ccccgggact tgacacattc gatgaaatcg ttgtgatgcc cccggctgcg
     4441 gcccagatgg atctcattgg gtccgatcac ttccctcagc agagactggg gctcggcttc
     4501 aagtgtgccc gcgtgcacat gaatgaatac ccagccctca tcaccttcga accggatccc
     4561 gcgcggaccc cggttgtcgc cgaccagccg gacgccgttg gcatattcaa aagcgaaatt
     4621 gtaggcatag gctgtattaa aaagaccctg tttcggaaat tcacccgtgc cttccacgct
     4681 gatcggcgtg gtgtcatcgg taccgttgcc gagctgaccc agatccaaaa tatgggcacc
     4741 gcggtcggac atctctccgg cgctgtaatc cagaatgtag cggaaataga aatggcagcg
     4801 tttttctgta taaggtgctt cccgggccgg gccgagccag aagtcataat caaaatgttc
     4861 aggcacgggc atttcagggt agatgatgcc atagtcgtgg tctaccggca tctgcacccg
     4921 gattgtatga agcttgccta tcctgccgtt acgcaccagt tcacaggcat agcgcgaatt
     4981 gtcccgggag cgttcgtggc tgccggtctg gaatactctc tggtactgac ggactgcatt
     5041 gactacggcg cgcccttccg ggatggaatt ggcgagcggt ttttcgcaat agatgtcttt
     5101 gcctgcctga gcggcagcaa tgctgatcag agcatgccag tgatcggggg tggcgatcat
     5161 gaccgcgtcg atgtcgtccc gggcgagcac ttccctgaaa tcatgataag ccgtgatacc
     5221 tttgaaagaa tcgctgtttt ttttgccctg ataaaaggct tcaacttcct ttttcgcatt
     5281 agcgcaatgt tttctgtcca cgtcacacag agcaacatat tgcacatccc cgatgctcat
     5341 aagcccccgg gtgtcgtaca tgccctgacc gcccacgccg atggatgcca tgacaatacg
     5401 ctcgcttggg ggcgtgtggc cgtctctgcc gagtgcagat gccggaatga cggccggaag
     5461 ggcaattgcg gccgctgcct gtttcataaa actgcgccgg gataaagaat tgtgttttga
     5521 tgccatgatt aagtctcctg aggtttttcc tattgttata cattttcctt aaagaaataa
     5581 agtttttgga aaagactgtt tgagtcctaa caatattgaa aattaaaggc cgttcaggta
     5641 tcatggagag gagaagaggg aacaggccgg taaaatgaag agaaagagtg atgaaattgc
     5701 aagagcctgg aaaagtacac tttctgttgt acctgcttgc ttcgctgctg ctgaagctgc
     5761 tgggctggaa agttgaaaac acaccgcccg ctgcaccgaa atacgttgtg attgtggcgc
     5821 cgcatacatc caactgggat tatcccatcg cactgctgat ttcctggaaa tacagaatga
     5881 aaggggcgtg gctgggcaag gaagagattt tcaggtggcc tgtactgcgg tggctttttc
     5941 gcgggaccgg aggaattcct gttgacagga agcatcataa caatctggtc gggcaggcgg
     6001 ttcatgagtt caacacgcgt gatgccctga tatttgcact ggcgccggaa ggtaccagag
     6061 cccgccaggg gtattggcga tccggctttt accatatcgc ctgccgggcg caagtgcccg
     6121 tagcgctggc ctatcttgat tatcgccgga aagcaggcgg gttcggccct ctttatgagt
     6181 gcactgggga cgtacaaaaa gatcttgcgt atataagtga cttctatgaa acgattacac
     6241 cacgccatcc ggaaaagcga acgcctgtct gcttcagaca gtaatgcact gcggaggggc
     6301 ttccacgccg caacgacact atgacgggca gtcacagggg aaagagcatt ggctttcagg
     6361 tcggacccga ttattttaca attagattga ccatctttcc gggcacataa atctctttaa
     6421 ccacggcttt tccttccaga tgaggcgcaa tcttcggatc ggtttttgca gcagtcaaaa
     6481 tgtcggcttc aggcgcactt gccggaacca taatgcgtcc gcgtaatttt ccaagcacct
     6541 gaaccggtat ttcaatgttg tcttccacca gcagggcagg gtcaaaagaa ggccaatttt
     6601 cataggcgag tgtggtgtct ttgcccattt tttcccagag ttcttccgcc agatgcggag
     6661 cgaaaggagc cagcagaagc agaaacgatt ccgcactttt cctgtcgaag gacgcggttt
     6721 ttaaaagagc atttacaaat tccatcatgc gtgcaatagc ggtgttgaag gaaagagact
     6781 cgatgccatc ggtaaccgct ttgatggttt tgtgcaattc acggagcaga gcgtcgtcac
     6841 ttgcctgatc ctgaattcgg ggatgcagct ttccgtcctc attgacgaaa gccgaccata
     6901 tccgacgcaa aaagcgataa acgccctgta cgccttcatc ggtccagggt ttgtcgcgat
     6961 ccagcggccc catgaacatt tcataaagac gcatggcatc tgcaccgtag cttttaacta
     7021 catcatcggg attaacaacg ttataccggg acttgctcat cttttcgatt tgcgtttcca
     7081 ccatttcgcc tgtggctttc agccgccagt ggtcaccgtc ccgctccact tcatgaggat
     7141 aatggtattt gccgttgctg tctttaaaag aataggcgag gatcatgccc tgattgaaca
     7201 gttttgttgc cggttctttg gtgtgcacat atccggcatc gtacagcact ttatgccaga
     7261 atcgtgagta gagcagatgc agtacggcat gttccgcacc gcccacatac agatccacag
     7321 gcatccagta acgttccgct tcttccgacc aggcttcgtt ttcattggtc gggtcgcaaa
     7381 aacgcaggaa ataccagcag gaacccgccc attgaggcat tgtattggtc tcacggatag
     7441 cgggttgtcc ggtttcagga tcggttgtgt tgacccatgc tgcagcccgg gcaaggggcg
     7501 gcgctccgtc cgcagtcggt ctgtagtcgt ccagttcagg cagaagcagc ggcaggtctt
     7561 tcatgggaag cgtcttgatt gtgccatcct gaaggcggat aagggggaag ggttcacccc
     7621 agtaccgctg gcgggaaaaa agccagtccc gtaatttgta gttgacagtt cccttgccca
     7681 gtcctttttc ttccagccag gcggtaatgg tctttttcgc ttcgtccaca tggagcccgt
     7741 tgatcagggg tgaatttacg agcacgccat cgcctgtcca ggcttctttt tcaatattgc
     7801 cgccggaaat gacttcgatc acaggaatat caaagcgttt tgcaaactcg taatcgcgcg
     7861 tgtcgtgggc aggcacggcc ataatggcac cgtagccgta ttccgccagc acgtaatctg
     7921 ctgtccaaat gggtacaaga gcgccattca caggattgac agcaaaggat ccggtaaaca
     7981 cacccgtttt ttctttctcc tctttcatcc tgtcctgagc acttttgttc gaggcggctt
     8041 ccacataggc ttttaccgca tccatctgct cagatgtggt gatttcaaga gtcagcgggt
     8101 gttccggtgc cagcacgcag tatgtcgcac cgaaaagagt gtccggtctg gtggtgaaca
     8161 cagtgaagac ctgacccgag ccggcgactg taaaattgac atccgcccct tcgctgcgcc
     8221 cgatccactc acgctgcatt gccttgatcc cttcgggcca gtcaagcaga tcgagatcgg
     8281 caaggaggcg ttcggcatac tcagtgattt taagcatcca ttgccgcagc gggcgttttt
     8341 ccacagggtc acccgtttcc acataccgcc cgtctttcac ttcctcattg gcaagcacag
     8401 tattcaacgc cgggcaccag tttaccggtg cttcaatttc ataagccagg ccgcgctcat
     8461 acagaatgga aaaaatccac tgcgtccatt tgtaatattt ggggtcggta gtgttgatct
     8521 cgcgatccca gtcgtaagaa agtcccagcc gttggatctg gcggcggaag gtgtcgcagt
     8581 tgctgttggt aatgatggcg ggatgttcgc ctgtgcgaac tgcgtgccgt tccgccggaa
     8641 gaccgaaagc atcccagccc atgggatgca gtacgttaaa cccgcgcatc cgtttataac
     8701 gggcgatgat atcggtggcg gtatacccct ccggatgtcc cacatgcagc ccgtcgccgc
     8761 tcggataggg gaacatatcc agtacgtaat atttgggctt gctgtggtcg attacggatt
     8821 taaaagtttt gttttccagc cagtatttct gccatttcag ttctatcgcc tgaggttcgt
     8881 atgctgctgt cattcctgcg aagtctccct ttgtctgctt tctgtctcgt ccgcatctgc
     8941 ggcacctgcc agttccagaa tctgctcttc catttcttcc gggaaagtga tcagttcgtc
     9001 cagaatgcct gccatccgaa caggatcact gtgcaccagt tcctgcatca accggagaac
     9061 aagccgcttg gtgcgaaggg tggcaccgga aaagtcggaa aaaattcgtt ccagatgagt
     9121 tgaataaata tcaaaaatcc gccgttcatt cgcttcctga ttgtctgcag gcgtgccgct
     9181 gtacggataa agtccggttt ctttccagta gtccagcact tcaattgccg attccgcttc
     9241 gcgaagagcg aagtgttcac gtagttttaa tcgtgatgcg ttcagcagct ggcggatgtc
     9301 gggatgaagt tcccctgttt caagaaagcc ttcactgtcc agtgcagcga tatgtgccga
     9361 tttcagataa gcggaatagc tgaatccgcg aaaatatata cgaggttgag cctgaatgcg
     9421 cataaatccg ttttcatcac acagcaggag ggcgcgtttc ccggggagac gccattccac
     9481 cacgtccaga gttgcttcaa tgcgcttccc gtcctccgtc accatctctc ccagggggta
     9541 agtggtaaat cgggtctcgg cattggaagg atccagaggt atgccgtcgt aaataatgtg
     9601 cgtatccgga tactggcgta aatagagggc aaacacattg gttacttctt ccattgcctt
     9661 tacgccgcgg agcagattgg atgtgtctct gacatcctga atttcaacag tcatgcctga
     9721 aggttgctgt acctgacctt tccggtcgaa aacagtaaat tctcccagtt tggcagcgtg
     9781 tccgctcagc ttgaatgaag cctgtgcggc accatctttg taaacagaat gccagcagac
     9841 actgttgccg agagaaaaag cgcggaagcg gccttttccg aatttcccgt gcaacacacg
     9901 cttacgctca tgggttcggg agccatggcg tttccaggat ccgcccaggt ttctgaagac
     9961 aagaagcgca tcgtcataat ggagcccgtg tccgttgtcg ctgatgcgga tcgtttccag
    10021 acccgtcagc tcattttcta taaacgcaac acgcacttcc gtggcttcag catcaagggc
    10081 attccagatc agctcagaca gtgccgccat gggctttgtc tgggcaagtg tctccagatg
    10141 gtcttcccgt acttcaacgg taattgtttt cacctgttgt ctcccaaacc gctattgaat
    10201 cagtatgtcg atacggctga aacactctgc tgccgcaacg gaaaataatg agttcacccc
    10261 gtaaaagaat accatttaca cagcggaacg cacaaattaa attgacaatc gaagttatat
    10321 atactgataa ttgaatgaga cctggacgca cggtcggaaa aatgtccgcc agatttgaag
    10381 tgtaaacgac tgggcagtgc aaaacttttt tgttgcagta ttaaagcgtc atgatgcagg
    10441 cacaggaaag agacgaatta tgaagcagat tattattgag cagccgggag tcgctcctgt
    10501 tactgccgta ctgtctacag gggagatccg cctcggaaga gcggaaaata atgatattac
    10561 gctcacggct gaagaagtgt cacggcatca tgcgaccttt gccatagtgg acgggcaatg
    10621 tgttgttact gacctcaaaa gcctgaatgg tacttacata aaccggcagc ggatcgctac
    10681 cggcattgcc cgccatatgg atgaagtctg gctgggcagc aagtgccgga ttgtctaccg
    10741 ggatgatgag acgcagcctg ttcccgttgc ttcatcgcag gagatcaagg agcgtctcgc
    10801 ccagagcatt cgccatattc gaagcgaaat gcagcaggta gacaagcatg tggcgcaaat
    10861 gaaggagcag caacagaaga cagacacaac actgatgggg gatgccagtg cttttacaat
    10921 gtcgccgggt gaggttgccc gcatgagcca tgcctaccga aggcttgatg cactattccg
    10981 ggcaggacag gctgttgcct ccaattttga cctgaagacg cgcctgaccg gcatgctcaa
    11041 gatcatgatg acgacgctga acgcatgccg ggggtttgtg ctgctgcgaa atgaaaagaa
    11101 tcacacgctg caggtagaag tgtcgcttca aatgggaacg gaactgaatg ccagcgctcc
    11161 cagtatgggg attgccggaa gggcagcctt cgatggagag cccgtactga tgaaagaccg
    11221 gcaggactgt atggagttcg gcggacggca aagcattatt gccaataaga ttacaagcgc
    11281 catgtgcgtg cctttacggg gaaaagaaca gctgctgggt tccatttatg tggataccga
    11341 tcaggataat gtcaatttca atgaagaaga tctggagtta tttttatctc tgtcagccca
    11401 ggcggcactg gcaatcgaaa atgtgcggct tcacgaacag accgtggagt ttgagaggaa
    11461 aaggggggaa ctctcccgat ttttgccggg gccgcttgtg gaaaaattga tgaatcagtc
    11521 cgaagcactg acgctcggag gcaataaaac aagagtgaca acccttttct gtgatatccg
    11581 tggttccacc catatagcgg aacagctgtc gcctcaggaa cttgtcacgc tgctgaatga
    11641 acattttacc gccatgactg aaattgtatt tgcatacggc ggcacactgg ataaatacat
    11701 aggcgatgaa atcatggcga tcttcggcac acccctcccg accggagacg aagaattccg
    11761 cgccgtctgt gctgctgtgg aaatgcagct atgtaacgtg cgactcaatg cagtgcgcca
    11821 gtccgaaggg cggttcccgc tgcagatagg catagggatt gatgtaggag tggtgatcgc
    11881 cggatttatc ggttcgccca aacgcatgga ctttacagtg attggagaca aagtaaatac
    11941 ggcaaaacgc ttttgcgata tggcgggacc gggtaaagtg gtggtcggag aagatgtgtg
    12001 gcagaaaata catgaccatg cacaggcaaa gcctttagga gctgtcgcct tgaaaggaaa
    12061 acagcatcct gtgcaatctt atgaaatact tacagtcaaa cgcccgaatc aggcatgacg
    12121 agacagttct taaaaatgag taaaaaacag cagacacaat tgactgaaca cataaaacat
    12181 ccggcatggc tgaccgggct tttttcacgg gaggcactgc ccggctgggt gatttttttt
    12241 cttgctcttt ttgtgcgtgc agtacatcag tggtttattc agcaaaacga tcccatgttc
    12301 gggtacctgc tcaccggcgg cgacaatcat acctttgacc gatgggcgct ggaaatatct
    12361 caaaccttct ggctgggttg ggatcgcacc ccattttttc atggtcccct gtatccctat
    12421 gttctgggtc tgttttacct tcgcttcgga tattcgcctc tgacggcggc gtggatgcag
    12481 cacggaatcg gttcgctcag cgccgtcctg gtcttctatc tcgcaaggaa ggccttcaat
    12541 caaaaaacgg gatggattgc cgggattatt gctgttttct gtcctatttt tctcttatac
    12601 gagtgcgaga ttctttccga tacgctgatt ttatttacga atctcctgct gttgtgggct
    12661 gcttttcatg cggctgaaag tcagacaaaa aaagcctggt ttctgcttgg gcttgcaacg
    12721 ggagtggctg ctctgggtcg acccaatgcg cttatcctga tacctgtcat tgctttatgg
    12781 ctttgggtca gtgtgaagaa gacctggaaa acaaagacgt ccgccgccgt gttgtttacc
    12841 tttaccgctg tcgcatgtat tcttcccgcc acatgcgcca attactttgc aggaaagcat
    12901 tttgctttgg tgagctggag cggtcctttc aacctctaca tcggcaatgc accggacggt
    12961 accggcgttt tctggtcgcc tccttccatg cagaaaatcc gcgcagaaga gaaaaagcct
    13021 gatcttgaaa ttaactggtc ccgctatatg aggcaagcgc tgctggatga tcccctcctg
    13081 ctgccgcgca gcctctggct gaaatttaga cttttctggc agagcggcga aattccgcac
    13141 aatatcaatg tctatctgaa aaagccgttc tcgccttttc tccggatacc gctgtattgg
    13201 ggtatgatcg cacctctggg tattctgggg tttttctgga cggtgctccg aaaaaagact
    13261 ttttcctgga cggatcccgt actgattctc ggcttttttt ttgccgtata tatggcaggt
    13321 attgtgctgg tttttgtgct ggcaagactt cgtatgcctg ctctggcagt gctgactatt
    13381 tttgcggcag gcgccctatc gcaaatgcat gatttatgca gagaagcccg ggtaaaaaga
    13441 agcatgcgcc ctgtggcaat gatcctgctg ctgctgcttt tgtggagctg tggtggtgtc
    13501 cttctgaaaa gccgtgatga tacgctgctt ttgcgctgga atgattattt caaccttggc
    13561 agtgcctacg aaagtaaaaa tagacggcag gaggcactgg agcagtacga gaaagccgta
    13621 gagcgcgctc cggaaatcat cgctattcag ggtgtacgtg atgaaatgcg ccgtcagttg
    13681 gagcagcgac agcagtaaga catctgctta tccctttctc tctcatcttt tacattggta
    13741 cggctgaaat agtataattc agaggcaaac ggcgtcgccg cagggtgtat tgccctgtta
    13801 tagggacgtg tatctggctt gaaggggaca gccgcaacgt gctgctctct caacacggca
    13861 gcagcactaa agcggctgac acgaacttcc gggtgtaaat cccatactaa acaggaaaaa
    13921 tagtcatgat tattgtcatg tccacgtcac ggcaggaaga tgttgatcat gttgttgccc
    13981 gcgtcgaaga attcggatat aagcctcatg tcatttacgg tgtagagcgt accgtggtag
    14041 ccgctgtcgg cgacgagcgc ggcaaagagc ggttaacctc tcttgaatct cttccttatg
    14101 ttgagcaggt catcagaata ttaaaacctt ttaaacttgc cggaagggaa gtgaagcagg
    14161 aaaataccgt catagaatgc gccggcgttc tcataggcgg aaatcatttt gcactgatgg
    14221 cggggccgtg ctcagtagag tctgaagaac agatcatgga atcggcgcgc attgccaaag
    14281 aagccggagc caatattctt cgcggcggcg cattcaaacc gcggacctct ccctacagtt
    14341 ttcagggcat ggaaatggaa gggctgcgtg cccttcgaaa agcggcagat gcctatcata
    14401 tgccttttgt gacagaggca accacgcctg agcagatccc tgttgtggcg cagtatgccg
    14461 acatgattca aatcggcgcc cgaaatatgc agaattacgg cttgctccgg gcggcaggca
    14521 aggcaggcaa acccgtgctg cttaagcggg gtatgatgtc cacgatcaat gaatttctga
    14581 tgtccgccga gtatattctc tccgagggca acccccatgt gattctgtgt gagcggggta
    14641 ttcgcacttt tgagactgaa accagaaaca cgctggacat acaggcggtt cctgttgtta
    14701 aattgcacag ccaccttccg gtgattgtgg atccgagcca tgccgccgga cgctgggaca
    14761 tcattattcc catggcgtgc gctgctgtgg cagccggtgc ggacggaatc atagtcgaga
    14821 tgcatcccaa tcctgaagtg gcattgtcgg acgggcagca gtctttgcgt ccgaaaaaat
    14881 tccaacggct ggtggaaacg ctcaaaccgc ttcttgccgt catgaaaaaa gaaatatcct
    14941 gaacggttgg aaggagatta gatgagccag gaaccgctgg agaaagacga atttccggag
    15001 attgtggagt cgctggggcg tgaagaatcg cgccggacgc tgtacgctct gctttttgta
    15061 agtgacaggc ctgttggtgt taaacgtctg gcggaagcgc tgggagacat ggatgaggaa
    15121 atggcgggtg tgctgctcaa tgaactgatg caggaactga acagccgcga agagctcccg
    15181 tactcgctcc agcagattgc cggagggtac cagctcatta ccaaaacgga atatgccccc
    15241 tacatccgga ggtttttcca gatcaaaaaa agcaaccgca tgagcaaggc attgctggaa
    15301 acactggctg tgattgccta taaacagcct gttacccgtg ctgaagtgga agcaatccgc
    15361 ggcgtaagtg tatcgtatgc ctttgatcag cttcaggaaa aacgcttcat ccggatcagc
    15421 ggtgtggcgg atctgcctgg acgtcccaaa ctgtatcgga cgaccgagga atttctgatg
    15481 gctttcgggc tgcagagcct gaaggaactg ccctcgcttg acgaactaaa aatcatggat
    15541 tgaccatggc acttatccgc ttgcaaaaat atctcgcaga aaaaggactg ggatcccgac
    15601 gcgcctgtga aagaatgatt cttgaaggga aggtggcggt ggacggcagc gtcgctgaag
    15661 tggggcagca gattgagccg gagcgccaga aaatcactgt caatggcatg ccgctggagc
    15721 aggatgaagc ggaaaaagtg tatatagtgc tgaacaagcc cgcccatgtc attactacgg
    15781 tgaaggatac ccacggacgc cggacggtca tggattgtct tcagggactt gacgaacggg
    15841 tttttcctgt cggtcgtctt gatcaggacg taagcggtgt actccttttt accaacgatg
    15901 gtgatctcgc ctatcagctc gcgcatccca agtttttggt tcacaaaacc tatcatgcct
    15961 gggtgaaggg gcacatatct gaagaggcgc tgcaatggct gcgcgagggc gtatcgctgg
    16021 aagacggctt cacggcgccg gcaaaagctt cattattaaa aaataaaaag ggagtatcac
    16081 tggttgaact gactatccac gaaggcagaa aacgggaagt caagcgtatg ttcaaagcgg
    16141 tgggttatcc ggtggaaaaa ctgcaccgaa cgacttttgc cgatatcaaa gcgaccgggc
    16201 tccgtccagg ggaatggcga cgactcagcg acttggaaat aaaagcactg cgccagtgcg
    16261 tcgcagcctg cagtgaaaaa caataaccat ggatcatttc gattccacct gcattaccag
    16321 actctgcaat gccttgcaca gtattgagga agatgccctg ccgcgctggg gcacgctaac
    16381 aaaatcggcg atgataaatc atttcatctg gatgatacgt cattccatgg ggcgttcttt
    16441 tctggtaccc gattacagta cttttctttc ccggacactg tcgaagaaac tggtgctttc
    16501 cggcgtgcgg gcactgccca aaaacaggtg tctgccggca catctctgca aacagggaat
    16561 tagcgatcag gaaacgggag atcttgaaac gcttcaggcg ctcatggagg agtatctgga
    16621 tctggtgcag acgggagatc tggaaccagc accccatccc aggtatggca ggctgaccat
    16681 tgatgaatgg gacagactcc atatgctgca ttttgagcat catttgcagc aatttaatcg
    16741 ttccatgcga taaatcccct ccggcttcac acccgcccga ccgattcacc ttctcagcat
    16801 ccggattttt acactggatc tatgtattcc ggagccatgc cgtcggaaca gaaaaaaccg
    16861 ggcaaccgca ggagtctgcg ccgccgtgct gttttgcagg cacgatgctt tgaaaagaga
    16921 ggggaagcgg tggtattcta actatggagt cctgctttcc aggcgggtga aaacggtaat
    16981 aatttactgc aatacaatct aacacccgca tctttctttt tatgcccgga gaaagtggtt
    17041 caaatgagca cagcaacaaa ggatctttcc tacattgagg aactggtggc gcaatacccg
    17101 gagcacaaag ggggaaccct tacaattatg cagaaaatcc aggaacggga aggctgtctt
    17161 tcgcctgaag cactggatga actggcgcgc tgctccggcg tttccgtcaa tgaactgtac
    17221 ggcgtggcta ccttttacag tttttacaaa tttagcccgc ccgggaagca taccattcag
    17281 gtgtgtctgg gtacggcatg ccatgtgagg ggaggcgcaa aaattctggc cgaactggaa
    17341 cggttgctgg gcattaaagt gggcgaagtg acggctgacg ggaaattcgg acttgtgcgt
    17401 attgcctgtc tcggctgttg tgcgcttgag ccggttatcg ccatagacgg caagttctac
    17461 ccccgtgttt ccgttcgcca actgccttcc attcttgcgg cctatcagga tcaataagga
    17521 acaggatcac caccatgact tttttaaata cccgctatga agcagcccgt aaagaatggc
    17581 aggatctgag agcagcggca catcctgtta tctatgttgg tgccgctacc tgcggcagag
    17641 cagccggtgc aggagcggtg attgattatc tttgtgatgc tgtcactgtt ggacaactga
    17701 ccgctcagat cattgaagtg ggatgtctgg gcccctgcta tctggagccg ctggttattg
    17761 tccagaagcc ggggatgccc cgtatctgct acggaaatat tggcattgcg gaaatggagg
    17821 ggctggtcgc ccgcttcctc aaaggagacg atgtttgcag acaatgggct ttgggtactc
    17881 ttgacgacgc cgaagttgac ggtatcgctc ccttgtctga gcacccggtc atggcacggc
    17941 aggtacgccg tattttcagc aactgcggta taatcgatcc ggagaatttt aatcattatc
    18001 ttgcccgggg cggttaccgg ggatttctgc atgcgctgga aattggcaga gaaaaaacgc
    18061 tggatgaagt ccggcgttcc ggtctgaggg gcagaggcgg cgcaggtttc cccaccttta
    18121 aaaaatggga gttctgcaaa aattctccgg gagaaaaacg ctttctcatc tgcaactgct
    18181 cggaaggcga tcctggctct ttcatgaacc gaagccttat tgaaggcgat ccccatgccg
    18241 tactggaagg tatgctgatt gccggatatg ccatgaatgc cacggcaggc tatgtgtatt
    18301 gcggtgtgga atatgcgacg gtattcaatc gcctgaatac agccatcgat cagatgcgtg
    18361 cacacgggct tctgggcgag gctgtcgggg gcagcgattt ttcttttgat attattgtga
    18421 aacgaggagc gggggcttat gtgtgcggtg aagaaacggc gctcatcgcc gccatagaaa
    18481 gccggagagg catgccccgg cccaggccgc cctttccagc ggcaagcggt ctctggggat
    18541 atcccacgaa catacagaac gtggaaacgc tgggcaacct gcctctgatc ctgcgcaacg
    18601 gtgcggattg gtataccgaa tgcggtacgg aaaacaacaa aggaacccgt tcgttttcca
    18661 tggcaggcag catacggcat cccggtctga ttgaagttcc gctgggcatg acgctgaaag
    18721 acatcattta tgatgtgggg gcggtgcgac tatggggcgt gccattaaag ccgtgcagac
    18781 gggcggaccc tccggcggat g
//
LOCUS       DAOPWB010000057        18686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3004_length_18686_cov_6.269604, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000057 DAOPWB010000000
VERSION     DAOPWB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 18686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18686
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3004_length_18686_cov_6.269604"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1137..3296
                     /locus_tag="PKN92_06975"
     CDS             1137..3296
                     /locus_tag="PKN92_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48571.1"
                     /translation="MLPADQPYTLMPEGVGGEAPSYSIIKTLAQRNGDSEPLINAYSS
                     EYERFSGKRNTFVFIDPYLFYHGTGYSHYGRVFLFRLIEYFPADFILRAWRATAVLFQ
                     LNNIYAASMPNADAFPGSVLSALIKLQRCLTDHTAHFGMAYFITALIGLASVNLPAAL
                     IISLFFLWFAGYPSLQYEFRHAFYLSFIPLLALGFCAENVLKISRAVFLNKTVHTSPL
                     SARIALRRAFLFVLLLLVSYALPLTILRAYQHSKVTALAEEIKKSPRSPVEVNISFRD
                     NRVVLSPQKPLQGLAASEGLLPGEVNWTYASLVFDSYGQNIPVTLHYKTDRFFNNFTH
                     SQVLEGCRDTKTGHIEFCFPIYETDNTYSMDMLPVFLQTYPVLREFVHDDTSLAEQNW
                     WRRGKFEGISFPEEYFPFFRGFTEIEAGDSVPILPFLTIPQEMQCLRTHKSGPWEQAL
                     SRWKNNTAPEQPALHFDTVWHIKDTFPTAKSWQLPKTPLLPSDSVNMLCNQWAELIHF
                     YPFLKSVAVEDMLNYAGMLKEKGQDWLAEESCKIAQKWADADPYPFMRLGELQEQNNE
                     AEQAITSYYKALLVYPYLPDTAERMDRLVLSLKDHKACADLWKKISAARPDDLFAGMR
                     LGLHYESASLYSEALLAFENVLQYHPDNPDVRLSLVRCFVTLGEPRKALPILKSVLDI
                     QPDYKVLVQQHAAMMAELLSSRGALSEAALFFELAAS"
     gene            complement(3357..5306)
                     /locus_tag="PKN92_06980"
     CDS             complement(3357..5306)
                     /locus_tag="PKN92_06980"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNZ48572.1"
                     /translation="MLDSGVMLIAGVLVLASVLASQVSDRYGIPALVVFLVVGMLAGS
                     EGPGALVFNDANFANFCGTIALAFILFSGGLDTHWTIISPVLWRGIFLATGGVVITAV
                     LVGIFSWLALGMTLLTGLLLGSIVSSTDAAAVFAIMRRSGVTLKGNLKPLLEFESGSN
                     DPMAVLLTMTFTTILTASNLHWGGLAATFVVNIFGGVLLGIGAGWLARLLFNKLHLGY
                     EGLYPVLSIGIVLITFGLSDFLHSNGFVALYVCGILLNSSDFRYRLAVIKFHDGLAWL
                     MQIMLFIVLGLLVNPSELLHVAGKALLVATFLMLVARPVAVFLGLWKSEFTLPEQLLT
                     AWTGLRGAVPIVLATFPLAAGFEGSSMIFNIVFFIVLTSVLIQGIMLMPVARFLKVDD
                     PMQARPQYSLEIQRSGQMQGETREIEVLPNMAAVGKTIGELDIPSEVLILLIGRGDDF
                     IVPKGQTVIEPYDILLLLGESGALKNAGEAILLPRSRSFPSVDLDDSLVMLPSNTARQ
                     FLSKQVVVVGYGRVGSRICACLKEKGIPFVVAEQDREIVERLRKDGIVAVSGDAASEM
                     TIAQAHVSKASVLVIATPDTMKVRRMAEIAKELNPSIEIIIRTGCEMEANLLKREGIG
                     EVFLGEEELAKSMAASALQSLKAKA"
     gene            complement(5411..5830)
                     /gene="nikR"
                     /locus_tag="PKN92_06985"
     CDS             complement(5411..5830)
                     /gene="nikR"
                     /locus_tag="PKN92_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNZ48573.1"
                     /translation="MSDLERISITLPKDLAKAFTASCEQKGYSNRSEAIRDLIRGALV
                     KEEWQDPAAQVAASLTMIYDHHTRQLNEKLTELQHDHEDLIVSTLHVHLNHDNCLEVI
                     VLRGEAQKVQDLSDKLRTFKGVKYAQVCFATDGSDLG"
     gene            6025..6990
                     /locus_tag="PKN92_06990"
     CDS             6025..6990
                     /locus_tag="PKN92_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNZ48574.1"
                     /translation="MLSSFRRGFTLIELLVVIAIIGILAAILLPALARAREAARRSSC
                     QNNLKQFGLVFKMYSNESPGETFPPCAPFGNGFMNGMTLFSAPSAFSIYPEYLSDLSV
                     AKCPSDAGVDGGGTYVATRLPSSGDFEQWVEAARASGDKMSENYYLCAQLGRSYLYKG
                     YVATSIEEYYGIWGAMCAKPFIEVVPILEISAPVRFKDFTGDLPITATGWPSNVDSSV
                     AQGTAQTDKVLRLREGIERFLITDINNASGSNSGQSAIPVMWDTYGNPTDSNATAGGV
                     VFNHIPGGCNVLYMDGHAAFVRYPVQFPITDDPGMVLEGSHFGLL"
     gene            7024..7854
                     /locus_tag="PKN92_06995"
     CDS             7024..7854
                     /locus_tag="PKN92_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNZ48575.1"
                     /translation="MTKRAISFFLVCIVLPLSAFASELGLLSLRFATTTSWLEVCLYD
                     LLGTEAKITRISPPGTCPGHFDIQPGVFSALQECDYLFFFDFQASIREKLSQGISGEI
                     RAISIPSPSGMSVPTQYTAVCESIHDILIQHFPEEQESLDNALASLKKRMADLENEIR
                     TLLKDASLTAQKVVASGHQRVFSSWLGFDVIDTFSGGDAASPRRLHSLVKKATKEKAD
                     FVIANLQEGSQYGQVLAEQLGIPLVVFSNFPSMEDSQRTFDALVLHNINQLLNAGKSR
                     "
     gene            7851..8648
                     /locus_tag="PKN92_07000"
     CDS             7851..8648
                     /locus_tag="PKN92_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48576.1"
                     /translation="MKNFARVESAMYRSQGKILVGPATFTVEKGECIGILGANGAGKS
                     TLLNFLNATLRPTSGSISLFEKNPWRLSEKERCLLRTKIGTVMQKCDYSSLAPFTVRD
                     LLSMGRLGFHGCAARLDTAEKNAIQDLLEEFGLAALADQPYRQLSGGEQQKAQIARAM
                     VQNPVVLLLDEPTAGLDPDWQEKLVLLIEALAAKRSITLLITTHAVHHLPRCCSRLFL
                     LKQGDIIFDGKRETALSDQLLSDLYNCPMEVLRHNNRSYCISKGDAL"
     gene            8649..9446
                     /locus_tag="PKN92_07005"
     CDS             8649..9446
                     /locus_tag="PKN92_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNZ48577.1"
                     /translation="MLFLWHAAALLSGSSIGLLGVYVVGMRMPFLGIGIAHAALAGAV
                     AGHVFGFPPFICALLLALIAALLMSWLTTSSAQADLGTVTGIMLSFTMGIAFLAMGFN
                     QGDMSPVLSMMWGSILFVRKTSLILMAVLSGVLFIFLYLCRLPMDSLLFSRTAARISG
                     MNEKKLLMVFMLISAFVITVNLQIVGGLLMYSLLTNPAAAAYALGRNMKSVRVFAMLS
                     GVVSTIGGLWISYLWDLSTGACIVLVSTALYSFALIATWKFKKQPLQ"
     gene            9470..10087
                     /locus_tag="PKN92_07010"
     CDS             9470..10087
                     /locus_tag="PKN92_07010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ48578.1"
                     /translation="MMHFQTDEELAIFFDQCAKDGLMLTFDPEEQSKLDCLLPEWDIR
                     EGHRIFEAGCGAGRLTEQLAPKIGISGEIFACDLSPEMIALAKQRKLSERVSFVCGSA
                     LKNNCPANWFNRILCLNVFPHFLDKPKTLREFRRIISRDGVMWINHLCSRESINHFHS
                     NASDEVANHILPDEEEMGVLLKEAGFGIMSLKDSDAGYSLKAIPV"
     gene            10191..11204
                     /locus_tag="PKN92_07015"
     CDS             10191..11204
                     /locus_tag="PKN92_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNZ48579.1"
                     /translation="MKRQGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQNN
                     LKQWGIIYKMYSGEAPGEKYPPIASWPFVAFGPEPSTIYPEYLTDPSIIICPSDSKGH
                     PSMLFCCDNPGDATWYSNPFQRTYHKGESWIEARAYMMQESYAYLGWVFDHLQDNPLY
                     LAPLSSCAPALSSLLPMAFPQIDPAIIAEANSSDVPIQFAVLFEALLPKLAPAILNSN
                     DRSLESPTKMAIDSDYAVPEPHGNGDGNTIYRLREGIERFLITDINNPAASAQAQSTV
                     PIMADLIGAGAGVSVFNHVPGGSNVLYMDGHVEFIRYPTKGPIIKPTASLLGLLASGF
                     DVN"
     gene            11723..13825
                     /locus_tag="PKN92_07020"
     CDS             11723..13825
                     /locus_tag="PKN92_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNZ48580.1"
                     /translation="MGIFIALLLVVGAAWGAGLALFSWIIGDAQHDVSEALANFRPKV
                     GTKVYSYDGQIIGEFSIEERSLVRLSDIPLHTQKAVLATEDSLFFEHKGVRPDAIISS
                     ILYGLQRGTSPRGGSTITQQVVRNVEDLKVGQERTYQRKLREALTALQIERNFTKDEI
                     LELYLNQIFLGGSAHGIEAASMQYFGKSCSFLTIAESATLAGLIRSPNRNSPFKNPDN
                     ALQRRNVVLKQMFENKFISKEEYDQAMQENLLASLVQPTVNRESIDYSVQGNYRAPYF
                     VEEIRNFVLQRYEKQEVFEEGFEIYTTIDMRIQSIAEETLLSALDEFDRKMNKGRSPE
                     DENYTAVSGALVCLDNRAPYRGQVRAMVGGRDFVREKFNTVTQAKRPPGSSIKPFIWA
                     AAIASGMTPSTIIVDEPFVRRAPNGKIWAPKNFGGGFSGPVPLRYALERSINIVSIKL
                     VERIGLPLIQSYLERCGIPTDVQNLTVGLGSGGVSPLSHAVAYSVFANGGMRYDPVLV
                     TEIFDRDGMQRYNYRDFSRAEQALDPKVAYVSLSMLEGVCKPAADYGYYPTAWRAHVL
                     NRPCGGKTGTTNDSRDAWFCGFTPEFTAVVWVGYRDNRSLGHGADYTGGRLACPIWVS
                     FMEQVHEGLGVEDFKVPPGIEFFDIDRKSGTRGGKYKEAYVQGTAPPLYTPPPPPAED
                     QGIMPEEAEEDIPLLLAL"
     gene            13954..15351
                     /gene="fumC"
                     /locus_tag="PKN92_07025"
     CDS             13954..15351
                     /gene="fumC"
                     /locus_tag="PKN92_07025"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNZ48581.1"
                     /translation="MTFRIEKDSLGEVQVPQSAYYGSQTARSLLNFKIGEEKMPVEII
                     YALAAVKAAAAEVHSEEGTLAQEKADAIVRACTEICEGKFHDQFPLSLWQTGSGTQTN
                     MNVNEVIANRASELLGGQLGAKSPVHPNDDVNKSQSSNDVFPTAMSIAAARLLHNRLI
                     PSLQRLHTALSQRAETFAEIIKIGRTHLMDATPLSLGQEFSGYAQQIENCIQRIESTL
                     PLLQELALGGTAVGTGLNSRASFGSKVAAVLSSKLGICFISAPNKFEALASHDALVMT
                     HGALKTTACALMKIANDIRWLASGPRCGLGEIQLPENEPGSSIMPGKVNPTQNEAVTM
                     VAAQVLGNDVTINVAGASGNFELNVFKPVIIHNVLQSIRLLADSADSFREKCIEGISA
                     REDRIAMHLENSLMLVTALNPVVGYDNAAKIARHAHLHHQTLREAALALNLITAEAFD
                     AAIQPAAMIHPAPDK"
     gene            15407..16189
                     /locus_tag="PKN92_07030"
     CDS             15407..16189
                     /locus_tag="PKN92_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNZ48582.1"
                     /translation="MNIFTWNVNGLRAALKNGFMDWFLSANADIVCLQETRLLPEELP
                     ASVSFPEAYHLSWNPAEKRGYSGTATISKEVPESVSGIGLEEFDSEGRVQILNYKKFT
                     LINTYWPNSQDERKRLPYKLAFAEAIGSKMDALVKEGKHVILCGDYNIAHEPIDLARP
                     KQNENSAGYYIEEREAMATFLGRGYTDVFRALYPEEVKYTWWSYRGGARAKNVGWRLD
                     YHCVNTAFMPFVKDICIHDDVTGSDHCPVELLLKDFQGRRTK"
     gene            16186..16989
                     /locus_tag="PKN92_07035"
     CDS             16186..16989
                     /locus_tag="PKN92_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ48583.1"
                     /translation="MKSFKTLVKVILLLLISLCFFALRFAFWGVLFRFPVVDRKTKRW
                     ILRYWARTFSFVIGMKRQTSGAVPAPPFLLVANHITYFDMLVLACETGCIFVSREDVE
                     HWPVIGTFAKSLHMLFIDRSKRRDALRVNRLISKALTEGDGIVIFPESRVSCGMAVEE
                     FKSPLLQPAVDLQIPVHYASISYRTPPSSPVEGAIVSWWRPESFVAHLIRFLHYQGAT
                     ALIHFGESPIPGTDRKVLAQELHAAVQAQFIPLRQAASTYNSSEFPVQP"
     gene            complement(16995..18017)
                     /locus_tag="PKN92_07040"
     CDS             complement(16995..18017)
                     /locus_tag="PKN92_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48584.1"
                     /translation="MAGRAEPVSDEYEFSVLKNFLAETLDLEYNRKIKEANLFELLNT
                     CAQRLYVALSKEKESLLKQEKAILSVEQEIKKRFLGYFIEKVFSCEENWETALTQEIA
                     RSGTGIIGTVFKWRYYLTSLPDLLSHLFKPEILQNAPSDAGSLQKLINEIKESETTLP
                     DTLKKFLAAMQRKTDYQMKLAGFSTRQSEEARVGYKEYFKEISRLFAQKAQDNLIKCS
                     KLFSSKYVKYALDSLPWMTILLSILFIIKSFYSQQLLSVSALLHLTALFVFVVFFVLK
                     IFNWVIASFSKKMLKDVRKAIEMHSTEQSSLLERERALIRDALRLIEKAETLMQECQE
                     AVYKES"
BASE COUNT         4718 a         4516 c         4202 g         5250 t
ORIGIN      
        1 gcctgccgaa gtcttattca attcaataat tgcatttcag ccttgaatcc gccagttgga
       61 taaatacttt tgttttgtaa cagataaaag cctgtttgtt tttatctttc atgcctctgt
      121 ataaaatgct caagaaattc ttaaaaagat gtgatttaaa cacttcgaat caagtactat
      181 tttctacagg gaaaacaacc gttcggcttt gcaagtatag cggaaggcga attttgccgg
      241 aaaagaatct ttcaagaata cccatcaacg catttttctg cgaattcttt ctgattgtgt
      301 tgcttctcag tgcgcaatta tatgtgctcc tgtccttcgc agcacaaaaa cctgtgctca
      361 tgaatacgcc ttttcaggcg tctttttcac ttcttactcc tgccgtatta tttgctggag
      421 gttacggtct cagcactact gacacagacg agatccctgg gctcaatgac tttattcttc
      481 gaaaagcaaa atcatttgat gtaaaacaat tccccgacag ctttgacaaa cagcctctta
      541 cgaaccctct tggcctggat cacctttatt ttttgtatat aataggctgg ctctggcgtc
      601 tttttggcgt gcatgaattt ctcctggtct acttgtactt ttttatgcgc ctgtgctgcg
      661 ggttgtttat ttacggtcta tgccgactgg gcacttctcg catcttttct tttttaacct
      721 ctcttcttat tgtgaccagt ccggcttata tggaccttga tatctcgcct cgagatttcg
      781 gtaaagcgcc ctttattctc gcttttatag tcgttggact atggctcgtc ttgcgcagtc
      841 attcatcttg gcgctttctt ttgttatctc tctttacagg caccttactt ggaattggct
      901 atggagtaag gccggatgta ttaatttgcc ttcccatggc gctgcttgta atcggcgttc
      961 tggcaaagat tcgagcaaga aaacgtcctc tctggcattt ggccggcaca attttattac
     1021 tggcctcctt tttcttttta acgaggccga ttttccaggc atcggcgctt gagggaaatc
     1081 atcgcctatc tgttcatctc atttgcatag gcatgttgca aagcatagaa aacaacttgc
     1141 ttcctgccga tcaaccttat acacttatgc ccgaaggtgt tggaggtgaa gcgcccagtt
     1201 attcaattat taaaacactc gcgcagcgaa atggtgactc tgagcctctg attaacgctt
     1261 acagttcgga atatgaacgc ttttcaggaa aacggaacac ctttgttttt attgatcctt
     1321 atctttttta tcacggaacc gggtattccc attacgggcg cgtgtttctc tttcgcctga
     1381 tcgagtactt tcctgctgat ttcattctcc gtgcctggcg tgccactgct gtgctctttc
     1441 agttaaataa catctatgcc gcctctatgc ctaacgcaga cgcttttcca ggcagcgtgc
     1501 tgtctgcgct tataaaactg cagcgctgtc tgacagacca cactgcgcat ttcggaatgg
     1561 cttatttcat tactgctctt atcggtttag catctgttaa cctgccggcg gcactcatca
     1621 tatcgctctt ctttctttgg tttgcgggat acccaagcct tcaatatgaa ttccgccatg
     1681 ctttttattt gtctttcatc ccactgctgg ctttagggtt ctgcgcagaa aatgtattga
     1741 aaatatcacg tgctgtattt ttaaacaaaa cagtacacac ttccccatta tctgcacgta
     1801 ttgctcttcg tcgcgctttt ctttttgtat tgttactgct cgtttcttat gctcttcccc
     1861 tgacgatcct tcgtgcatat caacattcaa aagtgacagc ccttgcagaa gaaatcaaaa
     1921 agagcccccg atctccggta gaagtaaaca tatctttccg cgataacaga gttgtcctgt
     1981 ctccacaaaa gcctctgcag gggctggctg cttctgaggg gcttctgcca ggtgaagtca
     2041 attggacgta tgcttctctt gtttttgaca gctacgggca aaatataccg gttactcttc
     2101 attacaaaac agaccgtttc ttcaataatt ttacgcattc acaggtgctt gaaggatgcc
     2161 gggacacgaa aacaggtcat attgaattct gcttccctat ctatgaaaca gataatacat
     2221 acagcatgga catgctgcct gtatttcttc aaacgtatcc agtactacgt gagtttgtcc
     2281 acgatgacac atctcttgca gaacaaaact ggtggcggcg aggtaaattc gaagggatct
     2341 cctttccgga agaatatttt ccgttttttc ggggatttac tgaaatagag gcaggcgatt
     2401 ctgtgccaat tcttcctttt ctaacgatac cgcaggagat gcagtgtttg cgtacacaca
     2461 aaagcggacc ctgggaacag gctttaagcc gatggaaaaa caacaccgca cctgaacagc
     2521 ctgcattgca ttttgacact gtctggcata ttaaagacac attccctaca gcaaaatcct
     2581 ggcagcttcc aaaaacacct ctccttccct ccgattctgt aaatatgctg tgcaatcagt
     2641 gggctgaatt aattcacttt tacccatttc ttaaatccgt cgctgtagaa gatatgctga
     2701 attacgcagg catgctaaaa gaaaagggac aagactggct ggctgaagag tcctgtaaaa
     2761 tcgcacagaa gtgggcggac gcagatccct acccattcat gaggctgggg gaattacagg
     2821 aacagaacaa tgaggcggaa caggccatta cgtcttatta caaagcgctt cttgtgtatc
     2881 cttatttacc ggatacagcg gagcgcatgg atcgtctggt tctgtccctt aaagatcata
     2941 aagcttgtgc tgatctatgg aaaaaaatca gtgctgcccg cccggatgat ctgtttgcgg
     3001 gaatgcggct gggacttcat tatgaatctg catctttata ttcagaagcg ttgcttgctt
     3061 ttgaaaatgt gctgcagtat caccccgaca atcccgatgt aagactgtcc ctcgtgcgct
     3121 gtttcgttac tcttggagaa ccccggaaag cactgcccat tttgaaatct gttctggata
     3181 ttcagcctga ttacaaagtg ctcgttcagc agcatgccgc tatgatggca gagttacttt
     3241 cctccagagg tgcgctttca gaagccgcct tattttttga acttgcagcg tcttaacgaa
     3301 aaaggaaccg gggcgcagag tgaccttcct cttgcgacct tgccgaaata acgatgtcac
     3361 gctttcgctt ttaaggattg caatgcggaa gcagccatac ttttagccag ttcttcctct
     3421 ccaagaaaaa cttcgcctat cccctcccgt ttcagcagat ttgcctccat ttcgcatcct
     3481 gtccggatga taatttcaat agaaggattc agttcttttg caatttccgc catacgtctt
     3541 accttcatgg tatccggtgt ggcaatcacg agtactgatg cttttgagac atgcgcctgc
     3601 gctatggtca tttcagaggc agcgtctccg gacacagcaa caatgccgtc tttgcgaaga
     3661 cgctcaacaa tttcccggtc ctgttcggcg actacaaaag ggatcccttt ttcttttaag
     3721 caggcgcaaa tacgggagcc cactctgcca tacccgacaa cgacaacttg cttcgataga
     3781 aactgccggg ctgtgttact gggaagcatc acaaggctgt cgtccaaatc aacagaggga
     3841 aatgatcttg atcggggcag caatatggct tcgccagcgt tctttaatgc gccggattcg
     3901 cccagcaaca gcaggatgtc ataaggctct atcaccgtct gccctttagg aactataaaa
     3961 tcatctccgc ggccaatcag aagaatcagc acctccgaag gaatatccaa ttcccctatt
     4021 gttttaccaa cagcagccat attgggcaag acttcaattt ctcttgtctc cccttgcatc
     4081 tgcccgctgc gctgaatttc caatgaatat tgcgggcgcg cctgcatggg atcatcgact
     4141 tttaaaaagc gcgccacagg catcagcatg atgccctgaa tgagcactga agtcaaaacg
     4201 ataaagaaca caatattaaa aatcatggag gagccctcga atcccgccgc aagaggaaaa
     4261 gtggcgagaa cgatgggtac ggctccccga agccctgtcc aggcagtcaa cagctgttcc
     4321 ggcaaggtaa attcactttt ccagagccca agaaaaacag caaccggtcg ggcaacaagc
     4381 ataaggaagg tggcaacaag cagcgccttc ccggctacat gcagtaattc tgaagggttt
     4441 accaatagtc ccagcacaat aaacagcatg atctgcatga gccacgccag tccgtcatga
     4501 aattttatga ccgccagccg gtagcggaaa tctgaactgt tcaaaagaat tccacacaca
     4561 tacaatgcca cgaaaccgtt actgtgcaga aaatcagaca aaccgaaagt gatcagcaca
     4621 ataccaatgc ttaaaacagg atagaggcct tcataaccca aatgaagttt gttaaaaaga
     4681 agccttgcca gccagccagc gccgataccc agcaatacac ctccaaaaat attgacgaca
     4741 aaagttgcag caagcccgcc ccaatgcaga ttggaggctg tcaggatggt tgtgaacgtc
     4801 atcgtcagca aaaccgccat aggatcattg cttccggact caaattcgag taagggctta
     4861 agatttcctt tcaatgtcac cccgctgcgc cgcataatgg cgaataccgc agcggcatcc
     4921 gttgaagata caatagatcc cagcagaagc ccggttaaaa gcgtcattcc cagagcaagc
     4981 caggaaaaaa tacctaccag cactgccgtg atgaccacgc cgcctgtggc aagaaagatt
     5041 ccccgccaga ggacagggga aataattgtc caatgcgtat ccagcccgcc tgaaaaaaga
     5101 ataaaagcca gtgcgatagt tccacagaag ttggcaaaat tcgcatcatt aaacaccagc
     5161 gcgcccggcc cttcagatcc tgcaagcatg cctactacga gaaaaacgac cagagccgga
     5221 atgccgtatc gatcagaaac ctgactggca aggacacttg ccagtactag cacacccgct
     5281 attaacatga caccggaatc taacatagaa aaaccatttt taactgcatt ttatgaagat
     5341 gttttttact aaacaggatc aggactgacc agtgcggaag aaagctaacg acgcctgtta
     5401 atcctctatt ttaccccaag tcggaaccgt ccgttgcaaa acaaacctgc gcatacttta
     5461 cccctttgaa cgtgcgcagt ttgtcagaaa gatcctgcac tttctgcgcc tctccacgga
     5521 gcacaatcac ttcaaggcaa ttatcgtgat taaggtgcac gtgcaaggtc gaaacaatca
     5581 ggtcttcatg atcatgctga agctcggtca gtttttcatt taactggcgg gtatggtgat
     5641 cataaatcat ggtcaaagaa gcagcgactt gggccgccgg atcctgccat tcttctttta
     5701 ccagagcgcc tctaattaag tcccttatag cctcagatcg attgctgtac cccttctgct
     5761 cacaactggc ggtaaaagct ttcgccaggt ccttgggaag tgttatagag atgcgctcaa
     5821 gatctgacac aataacattc ctcctactgc tgaaaatatc tcttttagaa tgaccatatt
     5881 atcctttatg aaaaaagaaa atcaagaaat aagagtgatt ttattaaaaa aaagcgtttc
     5941 actcagaagt tgcaaagtac attgttgaaa tgataatatt attttagtat ttgtaatacc
     6001 gtcttatgct gtagggatac taaaatgctc agttcttttc gtcgcggatt tactctaatc
     6061 gagttattgg tagtcattgc cattatcggt attttagcgg ctattctgct gcctgcgttg
     6121 gcaagggccc gtgaagcagc ccgacgctcc tcctgccaga ataatctgaa gcagtttgga
     6181 ttggttttca aaatgtattc caacgaaagc ccgggagaga cttttccgcc ctgtgctccc
     6241 tttggcaacg gttttatgaa tgggatgacc ttattcagtg cgccatctgc tttcagtatt
     6301 taccctgaat atctcagcga tctttctgtg gcaaaatgtc cgtctgatgc cggtgttgat
     6361 ggaggaggga cctatgtagc gacccgcctt ccttcgtccg gtgattttga gcaatgggtt
     6421 gaagcggcac gtgcttcagg cgataaaatg tcggaaaatt attatctttg tgcgcagttg
     6481 ggccgctctt atttgtataa gggctatgtg gcgacttcta ttgaagaata ttatggtatt
     6541 tggggtgcca tgtgcgccaa accttttatt gaagttgtgc ctatcctcga gatttccgct
     6601 cccgttcgtt ttaaagattt taccggtgat ctgcctatca ccgccaccgg ctggccctct
     6661 aatgtcgata gttccgtagc ccaagggaca gcgcagacag acaaagttct caggcttcgc
     6721 gaaggtattg aacgtttttt aattaccgac attaacaatg caagcggcag caacagcgga
     6781 caaagtgcaa ttcccgtgat gtgggatacc tatggcaatc caacagacag caatgcaacg
     6841 gcgggaggtg tagtatttaa tcatattccg ggtgggtgca atgttcttta catggacgga
     6901 catgccgctt ttgtccgtta tcctgtacaa tttcccataa ccgacgatcc aggaatggtt
     6961 cttgaaggca gccatttcgg gctgctttaa aatcgccttg ctcacttaaa agcaacttac
     7021 acaatgacaa aacgcgctat ctcctttttt ttagtttgta tagtcctccc gctcagtgcc
     7081 tttgcctcag agctaggtct gttgtctttg cgctttgcta ccaccacttc gtggctggaa
     7141 gtgtgtctct atgacctgct cggcacggaa gcaaagatta cccgcatcag tccgcctgga
     7201 acgtgtcccg gacatttcga tattcagccg ggtgtctttt ctgcactgca ggagtgtgat
     7261 tatctgtttt tctttgattt tcaggcctcc atcagagaga aattatcgca ggggatttca
     7321 ggcgaaatac gggcaatttc catcccgtcc ccctcaggca tgagtgtacc aactcagtat
     7381 actgcagtct gtgaaagtat ccatgacatt ctgattcagc attttccgga agaacaggaa
     7441 tcgctggaca atgcacttgc gtccctaaaa aagcgtatgg ctgatcttga aaacgaaatc
     7501 agaactttgc ttaaagatgc atctttaaca gcgcagaaag tggttgcatc cggtcatcag
     7561 cgcgtttttt cctcctggct cggtttcgat gttatcgaca ccttttccgg aggagatgct
     7621 gcctcacccc ggcgtcttca cagccttgta aagaaagcga caaaagaaaa agcagatttt
     7681 gtgatagcaa accttcagga aggatcccag tatgggcagg tgcttgccga gcagctgggt
     7741 attcctcttg tcgtattcag taattttcct tccatggagg acagccaaag gacatttgac
     7801 gctctggtac tgcataatat taatcagttg ctgaatgccg gaaaaagccg atgaaaaatt
     7861 ttgcccgggt ggaatcagca atgtaccgaa gccagggcaa aatacttgtc ggtcctgcta
     7921 ctttcactgt tgagaagggc gaatgtatcg gaatcctcgg ggcaaacgga gcaggaaaaa
     7981 gcacactgct caatttctta aatgccactc ttcgccccac gtccggctct atttcgcttt
     8041 ttgaaaaaaa tccgtggaga ctgtcagaaa aagagcggtg ccttttgcgc acaaaaattg
     8101 gaactgttat gcaaaaatgt gattacagtt ctctggcacc ttttaccgta agagacttgc
     8161 tttccatggg ccggttgggt tttcatgggt gtgccgccag attagatacc gctgaaaaga
     8221 atgccatcca ggatcttctc gaagaattcg ggctagccgc tcttgccgac cagccttatc
     8281 gtcaattgtc gggcggagaa cagcaaaaag cgcaaatagc ccgtgccatg gtgcaaaacc
     8341 cggtcgtgtt gctattggac gagcctacag cgggccttga tcctgactgg caggaaaaac
     8401 ttgtactttt gatagaagca cttgcagcaa agagaagcat caccctgctc ataaccacac
     8461 atgcggtgca tcatctgccc cgttgctgca gtcgtttgtt tcttttaaaa caaggagata
     8521 ttatttttga tggaaagcgt gaaacagcgc ttagcgatca actgttaagc gatttatata
     8581 actgccccat ggaagtactg cggcacaaca acagaagtta ctgcatttct aaaggagatg
     8641 ctttgtagat gttgttttta tggcatgctg cggcacttct tagcggttcc tccatcggtt
     8701 tgctcggtgt ttatgttgtg ggcatgcgta tgccctttct gggtatcggt attgcccatg
     8761 ccgctttagc aggcgctgta gccgggcatg ttttcggatt tccccctttt atttgcgctc
     8821 tccttcttgc acttatcgct gccctgctta tgtcatggct taccacaagt tccgcccagg
     8881 cagatctggg tactgtgacg ggtatcatgc tttcctttac catgggcatc gcttttcttg
     8941 ccatgggctt caaccagggg gacatgtcgc ctgtactgtc gatgatgtgg ggcagcattc
     9001 tttttgtccg caaaacttct ctgatactaa tggctgtact ttcgggagtg ctctttattt
     9061 ttctttatct ctgcagactt cccatggaca gtcttctttt tagcagaaca gccgcccgca
     9121 taagtggcat gaatgaaaag aaacttttga tggtgtttat gcttataagc gctttcgtca
     9181 ttacagtcaa cctgcagatt gtgggtgggc ttttaatgta tagccttctt accaatccgg
     9241 cagcggccgc atatgcactg ggcagaaaca tgaaaagcgt ccgggtcttt gccatgctct
     9301 ccggtgtagt aagtactata ggcggtcttt ggatttccta tctgtgggat ctttcaacgg
     9361 gcgcctgtat tgttctggtt tctacagcgc tttattcttt tgcacttata gccacctgga
     9421 aatttaaaaa acagccgctc caataaatcc tttaaaggaa aacagtaaca tgatgcattt
     9481 tcaaacggat gaagaacttg ccattttctt tgatcaatgt gcaaaagatg gcttaatgct
     9541 aacctttgac cccgaagaac aaagcaaact agactgctta ctgcctgaat gggatattcg
     9601 ggaagggcac cgcatttttg aggcaggctg cggtgccggt cggcttacag agcagttggc
     9661 acctaaaatt ggtatcagtg gcgaaatttt tgcctgcgat ttgtctcctg aaatgatagc
     9721 actcgccaaa cagcgtaaac tttccgaacg agtttcgttt gtttgtggat ccgccctcaa
     9781 aaacaattgt ccagccaatt ggtttaaccg cattctttgc ttgaatgttt ttccacattt
     9841 tttagataaa cctaaaacac taagagaatt taggcggata atttcccggg atggcgtaat
     9901 gtggatcaac cacttatgca gccgggaaag tatcaatcac tttcactcaa acgcgtctga
     9961 tgaagtggca aaccacatcc ttccggatga agaagaaatg ggtgtgttgc taaaagaagc
    10021 cgggtttggt attatgtctt tgaaggacag cgatgcagga tacagtctga aagccattcc
    10081 cgtgtaattc tttattttcc atcaaacttt ccgcatgcac ttgtctttta tcttgcataa
    10141 tgtaataaga tacagtgcgt tttaattttt tatcttttaa ggaaaagaaa atgaaaagac
    10201 aaggctttac actcattgaa ttgttggtgg tcattgccat tattggtatt ttggctgcca
    10261 ttttactccc cgctcttgcc cgcgcccgtg aagcggcacg aagatccagc tgccagaaca
    10321 acctcaagca atggggtata atttacaaga tgtattccgg cgaggcgcct ggggaaaaat
    10381 atcctcctat cgcttcctgg cccttcgttg ctttcggacc cgaaccctcc accatctacc
    10441 ccgaatattt aaccgatcca tccattataa tttgtccttc cgattctaaa ggacatccca
    10501 gcatgctttt ctgctgcgat aatcccggtg atgcgacctg gtacagtaat ccttttcaaa
    10561 gaacctacca caaaggggaa agctggattg aagcacgggc gtacatgatg caggaaagtt
    10621 atgcttatct cggctgggtc tttgatcatt tgcaggacaa tccgctttat ctcgcccctc
    10681 tgagcagttg tgcacctgcc ctgtcctcgc ttcttcccat ggcatttccg caaattgatc
    10741 ctgcaatcat cgctgaagca aacagttccg atgttcccat ccagtttgcc gtcctttttg
    10801 aagccttact gccgaaactt gcacccgcta tcctgaattc caacgaccgc tctcttgaaa
    10861 gccctaccaa gatggcaatt gacagtgatt atgctgtacc ggaaccgcac ggcaacggcg
    10921 acggcaacac tatttaccgt ttgcgtgagg gtattgagcg ttttcttatt actgacatta
    10981 ataacccggc tgcatctgcg caagcacaga gcaccgttcc gattatggct gatctaatcg
    11041 gcgccggggc aggtgtcagc gtctttaatc acgttcctgg aggcagcaac gtcctttaca
    11101 tggacggcca tgtcgaattt atacgctacc cgaccaaagg acccatcatt aaacccacag
    11161 ccagccttct cggtcttctt gcttccggtt ttgatgttaa ctgagtattt tcgaaagtga
    11221 ggcttttttt gccttcttta tccaagcggc aaatcagcct gttatcttat tttgctctga
    11281 ttttatatgg gcttctgttg cttctcgctt ccgtgcttcc tgtgcctgaa gcagatacat
    11341 ctgttttttc tgacaacgat aaactggttc attttctact ttacggtttt ctggcagcac
    11401 tcatttttct ttgcctgcag aatactgatg acaccattgc ttccggggct gtttgtttgt
    11461 attctgctgc agcatcttct ttctacggaa tcctgcttga atgctgccag tattttctcc
    11521 cttacagatc tttcgacata ctggatatag gtgccaatac tgcaggagca cttgttgtct
    11581 cctgcttttt tttattattt cgccgcgtgt tataattcaa tcagattgtc gtattctcgt
    11641 ctccttatct tctccattct gaaatagttt ccgtattatg actgaatcac atccacctat
    11701 gctgaaaacc aaaagaggct gcatgggcat ttttattgct ctgcttctag tggtcggtgc
    11761 agcctggggc gcagggctag ccctgttttc ctggattatc ggcgacgcac agcatgacgt
    11821 gtcagaggcc cttgctaatt ttcgtccgaa ggtgggcacc aaagtctaca gttatgacgg
    11881 tcagatcatc ggcgagtttt ccatcgaaga gcgctcttta gtccgtttat ctgatattcc
    11941 tcttcatacg caaaaagccg ttctggcgac ggaagattct cttttcttcg agcacaaagg
    12001 cgttcgcccg gatgccatta tcagcagcat tttgtatgga cttcagcgcg gcaccagccc
    12061 tcgcggcggc agtactatca cgcagcaggt cgtccggaac gtagaagatt taaaagtcgg
    12121 acaggaacgt acgtaccagc ggaagttacg cgaagccttg actgcgcttc agattgaacg
    12181 gaattttaca aaagatgaaa tccttgaatt atatctgaac caaattttcc tgggcgggag
    12241 tgcccatggt atagaagctg catccatgca gtatttcggg aaaagctgtt cctttttaac
    12301 catcgctgaa tccgccactt tggcaggatt gatccgctca ccgaaccgga attcaccctt
    12361 caaaaatcct gacaatgccc tgcagcgtcg taatgtcgtt ctcaagcaaa tgtttgagaa
    12421 taagtttatt tcgaaggaag agtacgacca ggctatgcag gaaaatcttc ttgcctctct
    12481 ggttcagcct actgtcaaca gggaatcgat tgattactcg gttcagggaa actatcgtgc
    12541 tccctatttt gtggaagaaa taagaaactt tgttttgcag cgctatgaaa aacaggaagt
    12601 cttcgaagaa ggcttcgaga tttataccac tatagacatg cgtattcaga gtatagcgga
    12661 agaaaccctt cttagcgcat tggacgagtt tgaccgtaaa atgaacaaag gccgttctcc
    12721 tgaagatgaa aactatacgg cagtttctgg tgctcttgtt tgtcttgata atcgcgcgcc
    12781 ttacaggggg caagtacgcg ctatggtcgg cggtcgcgat tttgtgcgcg aaaaatttaa
    12841 tactgtcacg caagccaaaa gaccgcctgg atcgagcatc aagcctttta tctgggctgc
    12901 tgctatcgcc tcgggcatga ccccatcgac gatcattgtt gatgagcctt tcgttcgccg
    12961 ggcccccaat ggaaaaatat gggcacctaa aaatttcggc ggaggcttca gcggacctgt
    13021 gcctctgcgc tatgcccttg aacgctccat caatattgtt tccataaaac ttgtcgaacg
    13081 aataggtctg cctctgattc agtcctatct tgagcggtgc ggtattccga cagacgtaca
    13141 aaatctaact gtgggtctgg gttccggtgg cgtatctccc ctcagccatg cggttgccta
    13201 ttccgttttt gccaatggcg gcatgcgcta tgatcctgta cttgtcactg agattttcga
    13261 ccgtgacgga atgcaacgtt acaattatag agatttttcc cgggctgaac aggctttgga
    13321 tccgaaagtt gcctatgtaa gcctctccat gctggaaggc gtctgcaaac cagcagccga
    13381 ttatggttac taccctacgg cgtggcgcgc tcatgtgctg aaccgtccct gtggcggaaa
    13441 gacgggaacc accaatgaca gccgcgatgc ctggttttgc ggttttacgc cggagttcac
    13501 agcggttgtg tgggttggct atcgggacaa tcgctctctt ggacacggtg cagattatac
    13561 gggaggccgc cttgcctgtc ccatatgggt cagttttatg gagcaggtgc atgaagggct
    13621 tggcgtcgaa gattttaaag tgccgccggg cattgagttc ttcgatattg acagaaaaag
    13681 cggtacccgt ggagggaaat acaaagaagc ttatgtgcaa ggcactgccc cgccactcta
    13741 tactccgcct ccgccacctg ccgaagacca gggtatcatg cccgaagagg cggaagaaga
    13801 tatacccctt ttgctggcgc tatagtcaat tccttcctgt gcggcgccct ctgttttgcc
    13861 ttagagcatg gcaccctgtc attttttatg ataccatatc tcaattcttc tttttgcaca
    13921 atttttctta actcctgaaa agaggattta ttcatgacgt tccgcattga aaaggactcc
    13981 ctgggagaag tccaggttcc gcagagtgcc tattacggct cccagactgc gcgttcgctg
    14041 cttaacttta aaatcggcga agagaaaatg ccggtagaga ttatttatgc attggctgct
    14101 gtcaaagcgg ctgcagccga ggttcacagt gaagaaggaa cccttgcaca ggaaaaagca
    14161 gacgctattg tgcgcgcctg tactgaaatc tgtgaaggaa aatttcacga ccagttcccc
    14221 ctttctcttt ggcagacagg cagcggaact cagaccaata tgaacgtcaa tgaggtgatt
    14281 gcaaacagag caagcgagct tttaggcgga caactcggag cgaaatcacc tgtacatccg
    14341 aatgatgatg taaataaaag tcagtcttcc aatgatgtgt tccccactgc catgagtatt
    14401 gctgccgcac ggcttttgca caaccggctt atcccctcat tgcagcgcct gcatacagca
    14461 ttgagtcaaa gagctgagac atttgccgaa attataaaaa tcggacgaac acacttaatg
    14521 gacgccacac cgctcagcct cggtcaggag ttttcgggct atgcccagca gatcgaaaac
    14581 tgtattcagc gcatagaaag cactttgccg ttacttcagg agttagcgtt aggcggcaca
    14641 gccgttggca caggattaaa tagccgggca agttttggca gcaaagtggc ggcagtactt
    14701 tcttccaaac ttggcatctg ttttataagt gcaccgaata aatttgaagc gctggcatct
    14761 cacgatgcac tagtaatgac ccatggcgcc ttaaaaacca ctgcctgtgc cctgatgaaa
    14821 attgctaatg atatacggtg gctcgccagt ggaccccgtt gcggcctggg agaaattcag
    14881 ttacctgaaa atgaaccggg gtcttcgata atgcccggaa aagtaaatcc cacgcaaaac
    14941 gaagcggtaa ccatggtggc agcgcaggtt ctgggaaatg atgtgaccat taatgttgca
    15001 ggtgcatccg gaaattttga gctgaatgtc tttaaacctg tgatcattca caatgtgctg
    15061 caatccatca ggcttcttgc tgacagcgct gattcattcc gggaaaaatg cattgagggc
    15121 atttctgccc gagaagacag aattgctatg catcttgaaa attcattaat gttggtgacc
    15181 gctttaaatc cagttgtggg ctatgataat gcggctaaaa ttgccagaca tgcgcacttg
    15241 caccatcaga ctctgcgtga agcggctctg gcattgaacc tgatcaccgc tgaagccttt
    15301 gatgcggcca ttcagcctgc cgcaatgatt cacccggcgc cggacaaata aattattgtc
    15361 acccgctgtt ttggcgaaaa tcagattaaa aagccaaagg aatgatatga atatttttac
    15421 ctggaatgtc aacggcctgc gagccgcctt aaaaaatggt tttatggact ggtttttgtc
    15481 tgcaaatgcg gacattgtat gtttgcagga aacccgactg cttccggaag agttgcctgc
    15541 cagtgtttct ttccctgaag cgtaccatct ttcctggaat ccggcggaaa aacgcggata
    15601 cagcggtacg gcgacgattt ccaaagaggt gcccgaatcg gtgtccggta tcgggcttga
    15661 agagttcgat tcagagggca gagtacagat tttaaactat aagaaattta ccctgatcaa
    15721 cacctactgg cccaacagcc aggatgagcg taaaaggctt ccttacaagc ttgcgttcgc
    15781 agaagccatc ggcagcaaaa tggatgcgct ggtaaaagaa ggaaaacatg taatcctctg
    15841 tggcgattat aatattgcgc acgaacctat agatcttgcc cgcccgaaac agaatgaaaa
    15901 cagtgccgga tattacattg aagaaagaga agcaatggca acttttctgg gtcgcggtta
    15961 cacggacgtt ttccgggcac tttatcctga agaagtaaag tatacgtggt ggtcataccg
    16021 gggcggggca cgcgccaaga atgtcgggtg gcgcctcgat tatcactgtg tcaatactgc
    16081 cttcatgccg tttgtaaagg acatatgtat ccatgatgat gtgactgggt ccgaccattg
    16141 ccctgtagag ctgcttctga aagattttca gggacgccgc acaaaatgaa atcctttaag
    16201 acgctcgtaa aagttatttt acttcttttg atttcgcttt gtttttttgc gcttcgtttt
    16261 gccttctggg gtgttctgtt tcgctttcct gtggtcgaca gaaaaacaaa acgctggatt
    16321 ttgcgctact gggcaaggac tttttccttt gtcattggaa tgaaacgtca aacctccggt
    16381 gctgtaccgg cccctccttt tttgctggtt gccaatcaca tcacttattt cgacatgctg
    16441 gttctcgcct gcgaaacggg ctgtattttt gtctccagag aagatgtgga gcattggcct
    16501 gtgataggaa cttttgccaa atcccttcac atgcttttta tcgaccggag taaacgccgg
    16561 gatgcacttc gtgttaacag gctcatttcc aaggcactta ctgaagggga tggaattgtt
    16621 atctttccgg agagcagagt cagctgcggt atggctgttg aagagtttaa gtcccccctt
    16681 ctgcaaccgg ctgtagacct ccaaataccg gttcactatg caagcatttc ctaccgtacc
    16741 cctccgagtt cgcctgtaga aggggcgatc gtaagctggt ggcgcccgga aagttttgtt
    16801 gctcacctca tacgtttttt gcattatcaa ggtgcgacag cgctgatcca ttttggcgaa
    16861 agcccgattc cgggcacaga ccgaaaagta ctggcgcagg aactgcatgc tgcggtacag
    16921 gcacagttta tccctctgcg gcaggctgcc tcgacctaca acagcagtga attccctgtc
    16981 cagccctgac ggaatcagct ttctttataa acagcttcct gacattcctg catcaatgtt
    17041 tcagcctttt caataaggcg caaggcatcc ctaatcagcg ctctttctct ttccaacaga
    17101 gaactttgct ctgtactgtg catttctatt gctttccgta catccttaag catttttttt
    17161 gagaaagaag caataaccca gttgaaaatt ttcaggacaa aaaaaacaac gaagacaaac
    17221 agcgcggtaa gatgcagaag ggcactcaca gaaagtaatt gctggctata aaatgatttg
    17281 ataataaata gaatggaaag cagaattgtc atccacggaa gactatccaa agcgtactta
    17341 acatatttcg aagagaataa tttactgcat tttattaagt tatcctgcgc cttttgagcg
    17401 aagagcctgc taatttcttt aaaatattct ttataaccca ctcgcgcttc ttccgactgt
    17461 ctggttgaaa atccggcaag cttcatttga tagtccgttt ttctttgcat ggcagccaga
    17521 aattttttta aggtgtctgg aagggttgtt tctgattctt taatttcatt aatcagtttt
    17581 tgaagactgc ctgcatcaga aggtgcgttc tgaagaatct ccggcttaaa taaatgggac
    17641 aaaagatccg gtaaagaagt cagataatag cgccacttga agacagtgcc gataattcct
    17701 gtaccgcttc gggcaatttc ctgagttaac gctgtctccc agttttcttc acaggaaaaa
    17761 actttttcta taaaatatcc gagaaaccgt tttttaattt cctgctcgac ggaaagaatt
    17821 gccttttcct gcttcaaaag actttctttt tcttttgata gagcgacata aagcctttga
    17881 gcgcaggtat tcagcagttc aaacaaatta gcttctttta tttttctgtt gtattccaaa
    17941 tctaaagttt ctgccagaaa gttcttaagc actgaaaatt catactcatc agaaacaggc
    18001 tccgcccggc ccgccactat tctgttccag gcgttaagag catttaccgg gaaatagtgg
    18061 cggacagaaa atccttcctg ttccatatag cgctgccagt ccttcctgac agcaggatca
    18121 tctctatcca cctgcgtctg cacacagaca atttctcttt tgttcttcag cggcgcaagg
    18181 atacgccgcg ttttgtcaac aagatatttc tgtgcgtcag cgcatagtag tataagatca
    18241 cactgtctca gtacctccag tgctgtttca cagtggcggg cattcgtact gtcccagtcc
    18301 ggcgtatcaa taagcacaat atttttcagc ggagagcccg gtacataagt gatacccggc
    18361 aagcagtcaa gtttaggaat gtatccgtgc ggatggtaga cagtcggtgt tgatgtatgc
    18421 gggcgaatcg gcgacacttc tgcaatttct gcccccgcca gcgcattgag caatgtggat
    18481 tttcctacac cggttccccc tacaattgca acaataagcg gagaatttat gccctgaagt
    18541 ctcgtcttta tatcttctat tgctatacaa aggttctctt tttcctgaag taatcttgtc
    18601 caggaaccag tgtaagcagc aaagctctca agcgtattta aaagagcgtc atattctttt
    18661 ggcacttttc cctgcagctc cccagt
//
LOCUS       DAOPWB010000058        18424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3062_length_18424_cov_5.586804, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000058 DAOPWB010000000
VERSION     DAOPWB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 18424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18424
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3062_length_18424_cov_5.586804"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1502
                     /locus_tag="PKN92_07045"
     CDS             174..1502
                     /locus_tag="PKN92_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNZ48585.1"
                     /translation="MSSSTRNSETNSQTTRRSFLQASTVAAAGAVLSTAPRSYAASEK
                     LAIHGGPAAIPNPDTDAWRWPRYGEVEEEALAAMLRSPDYSDIAALEQEWLEQTGGSY
                     AKAYCNGTSVLLSMYFALNLPPGSEVMVPSYTFFASIVPMRLFGLVPVFVDINPRTLN
                     FDLEDAKKRLTPNTRAILPVHWIGLPCDMDAISDWAKEKGLIVLEDCAHAQYAKLKGQ
                     HMGTFGEMSIFSYQMSKPLPGIEGGMGLYATQEYHDRATIFGHYDTPGIPDDSPYKKY
                     HGTGLGTKTRMHPLAAKLVRLQMRELKERVAAGVAQTRRLNDVLTQLPGLYEQTSGRN
                     DVERLYYEWNMLFIDEKEAGVSRQAVVDALRAEGVRADALAYRLQHEQPLYKDAEWWH
                     HAPVIPELPGSAAANATSVALPYFTTDAPEMVEGYIRAFEKVWAHRKDLA"
     gene            1606..2484
                     /locus_tag="PKN92_07050"
     CDS             1606..2484
                     /locus_tag="PKN92_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ48586.1"
                     /translation="MKVICRRYAVVSLVLTITAFAFFSCACLAEKTCAEGSPEPLRLA
                     CRVANYGKFQDQALEHISSMGLRYVFMSIPKAEEAEAVLAKLKALNLEALVVRGDTNL
                     STDASVDELRTQFETCKRMGVHYMFLSPKRHDAPKEDVYARLRRAGDAAADNDVILVL
                     ETHPDLGTNAAAHLETMKAINHPNVRVNFDSANILYYNEGADTVEELKKCIDYVATVE
                     VKDHNGEPESWFFPALGDGIIDFPAFFAVLREHGYTGPVTIEVEGIKGVEWSAADTCE
                     AMEKSVQYLKRIEHFK"
     gene            2564..4333
                     /locus_tag="PKN92_07055"
     CDS             2564..4333
                     /locus_tag="PKN92_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNZ48587.1"
                     /translation="MSTAGTLQMPDYFMIIGYFVLMLVIGLYFYRAMRDVKEYFSGGN
                     SIPWWMSGISFYMSSFSVAAFIFYPSLCYRYGFVGITLLWVAIPATLFGVLFLAVKWR
                     RARISSPVEYLEQRYSPLLRQIFAWQGVPVKVIDDGIKLFAIGTFMSVSLNMDITTSM
                     VAGGVIMLIYTFMGGLWAVNVTDFIQFIILTAAIIILLPLSFYHAGGWNAVSENMPEG
                     FLKFTNAEYGWGYVAFLIVMYSLSWSSNNWALIQRYYCVPKEKDAIKVGVLVIVLYLF
                     GPPLMFMPAIAATQIFPEQLADAGTVYPMLCKTLLPAGMLGLVVAAMFAATMSTLSGD
                     YNVCASVLTHDIYQRLFRPNASEKELVNVGRIATLLVGVIALGTALLMARGKGEDLFR
                     IMVTLFGVATAPVAIPMIMGLLSRRASARGALTGFLAGLTVGLVLFALSRYSKPLQWG
                     EAAAWLPESKELMVLGYKAKLEMVLFLASGFVTLLVMLADRWLISMSPVEKQRIDNFY
                     HRLSIPIGEMPGEEDIETRSAGVSPFGIVGISLFFTGLLMICVLPWAGLLMTALLNGT
                     IAVILLAAGAILAWPKKKNRSKA"
     gene            complement(4323..5111)
                     /locus_tag="PKN92_07060"
     CDS             complement(4323..5111)
                     /locus_tag="PKN92_07060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HNZ48588.1"
                     /translation="MPSLALPLKIAANTLLRKMGYEIRKFSAPEIRDFFPIDFDDWDK
                     DLWTAVAPHTMTSLERIYSLRRAVEYVTAAGLPGAFVECGVWKGGSSLAMTLCLQRLH
                     ADKRELFLYDTFDDGWPAGDEIDVTADGVPAHQLWLDALERGETADTLFAKYDNVRDL
                     LWSTGYPREQLHFIKGKVEDTIPGTIPEQIALLRLDTDWYSSTKHELEHLYPRLVSGG
                     VLMIDDYASFAGARKATDEYFETKGITILLHRVDDKGYRIGIKP"
     gene            5308..5478
                     /locus_tag="PKN92_07065"
     CDS             5308..5478
                     /locus_tag="PKN92_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48589.1"
                     /translation="MISTATCKTLLPGVGIVAGMLFLFIPAQAYCANAEGVFLLFGLF
                     CICSCLPVPTVR"
     gene            5627..6385
                     /locus_tag="PKN92_07070"
     CDS             5627..6385
                     /locus_tag="PKN92_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNZ48590.1"
                     /translation="MSASTILQELREMSNYLGAEHRGYAIIGEGNTSARIDKDRLFVK
                     ASGTCLATMGPADFLEVSISKVTRLLDSKEATDDDVRETLKSALTDPAETRMPSVETM
                     LHALLYAYPEYNFIGHTHPVFTNSVLCSVHAEEAMLGRLCPDHIVVMGRATVFVPYVD
                     PGLVLAREVRDRVRAFIDKENQPPKAIALQNHGLFALGSNARAVMNITDMAEKMSHIL
                     VGAYSMGGPRFMAAADIDRIDSRPDEKYRQERIA"
     gene            6448..8064
                     /locus_tag="PKN92_07075"
     CDS             6448..8064
                     /locus_tag="PKN92_07075"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNZ48591.1"
                     /translation="MSKLDTYRQAQQALPANYEAWQVFGAGLENVGRDGKSVTVPMRA
                     PAANEVLLRIDALGLCLSDMKIINQGGNHPRLRGRDLQNDPTVLGHECAATIVAVGEN
                     WKDQFQPGQRFIVQADIYYKGIGYAFGYMIPGGLAEYAFLDERGLAGDEGCYLLPVKE
                     STGFSEAALSEPWACVVMSYALDERVTPTGATQLVVADDPADWAEKMPRAQRVPKTLE
                     GLADDARFDDIILVAPTPALVETLGDRLNPNGIMFLLGDPAEDGDTQIDVGAIHYQNK
                     RYLGGGSTLEEVAEANRRIDLLPGGTSLFIGAGGPMGQMHVQRAIEMRNGSGCVVVTD
                     LDRGRLTHIEKRFGDLAKERNVKLITLAPGDFEDQQAMNLRMAALGGANGYDDVVVLA
                     PVPALVKQAVELSAKNAFVNLFAGLATGTRASVSLRQICRGVKMIGCSGSRIKDLRDV
                     LNMVEAGTLDSNRSVAAIGGLDAAHKGLAAVKNAVYPGKTVIYPQIPSFPLTAIDELV
                     AKEPEIAAALGTGGVWTNEAEKLFLEKYLP"
     gene            complement(8082..8804)
                     /locus_tag="PKN92_07080"
     CDS             complement(8082..8804)
                     /locus_tag="PKN92_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNZ48592.1"
                     /translation="MNKRVVFFSIATLNFLLLVATSAPASAADSLLHPADKNGDWKIS
                     QEEALSFLQSWQQEETPLVYAIQAAKLWQSGGWYRFLANRPAPRCWTSGTGTEGDGGE
                     AGWLALGSAVSRSGAAIEVPVVFKKGTADASVLLFQFTYDPEQLLLEEVLPGDALAGT
                     SKQIDAHEAQAGRLTLAIYGGQSAIASGQTARLHFRIREETPDGTTALHAVELSGATA
                     AAQPLFPAFRSGAVFTTAPRSK"
     regulatory      8905..9154
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9196..10614
                     /locus_tag="PKN92_07085"
     CDS             9196..10614
                     /locus_tag="PKN92_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48593.1"
                     /translation="MSFPFVLPAKHYPDDDASLNEGSIMVLRFFSSLSALLLLLLSPV
                     FSAGAQSPVPYNQATVETLYPFPLTMDAALISFDWDATGALHYTVGDPNWGTKLEVYK
                     LNGSVPQQIFASSSVWAGSRLTRLGAYMFFNDGGDFVRSDFNYYGYSASGSEPVFPLL
                     EAPFEASLWGLETRNEGEFFASGSATTWRPGALFYGQMDGAGAFSARPLVQFGEVGNS
                     PGPLCFDAQGTLYYVPGYAFAGGASIYRWTAEEVADALADPVTKSLAPDNHLWNTLPA
                     PYDGATGMACDSNGNVYVTATAWGSPSQFLLFNASDASPILVADYAGRLETLRYRDNS
                     LAVSCASGIFSIPLLRVESGTPSQVITGKVGETAVLSVQTRGGIGERHYQWFERSQNK
                     ADIVLGDDLSFLALPIEEESGDRAFYCQVSDATGTVTSELFTLVVEKSVPAYTVYGLA
                     VAVLLILAAGWILLQRQLLCGR"
     gene            complement(10559..12175)
                     /locus_tag="PKN92_07090"
     CDS             complement(10559..12175)
                     /locus_tag="PKN92_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNZ48594.1"
                     /translation="MKKWAGRTVFFLLTLLLWMICFCVVLEGWTRWRWKQIEATNPFL
                     LSLVAGELWPIIRDKSDDFSDGLYDEVLRANYRGPGKSMERLPEPTAEEEMRRRILYF
                     MRMPQEDRQMFANVFEFHIHAIRKDGIIAATFSEPQVKKQQPLNAFLGEAAVRPLLDA
                     FDEVRTGGEIRFFPKTEGETAGPFGYCLFSENTFEEETGEPLVWVIYPKTEIWRAVPE
                     DSLWEMPFFIYKKHVERQDIISVLAITEHFYMNNYGFRDADIYVPGPEDSYRILCLGA
                     STTEEGPVNELTYPAILEMRLNQHFGGKKIDVINCGLSGMNSSKHKMRMADYLALEPD
                     MILIYNAVNDICHELIPGWVNRATRFQQYLRKSQFVIRYLGKWIMPPEQEMAADIDCF
                     KIENLRQMEAYAREKGVALALCSFAAPNRERLNALELDYYEYTTRMKWGGRYISFDMY
                     LHILKLYNEAIKKLCAERGLLYIPLAEKLDGTANLFGDICHQRNPGIEKKADIVCEAL
                     IPVLEKELHRTAITDRTEAAAEEESNPQPE"
     gene            complement(12172..14376)
                     /locus_tag="PKN92_07095"
     CDS             complement(12172..14376)
                     /locus_tag="PKN92_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48595.1"
                     /translation="MANANSSSQLISPDSTHPARGTTALCFMLILLLGAVIRFQHLTA
                     ESVWWDEFATVAFLDPPEPWTQSPHYRQWNQQVERLDRPTLKQFLQQNQLVDPAAMPL
                     YLVLEFAWNRYVHASPAALRILSVCVGLLLLPLIYLIGSRLYDRRAGLIAMACLSLSP
                     IHVQFAKEIRMYGLLALLALITVYAFIRALEEGKKRWWFLGLTATCLLSWTHPFALLL
                     LFVQGLFWLLTRPRDIVRLTLWSLAVCLAVMPAVYYVATIQFWGQDSTDQWLRLPTVM
                     EVAADIFADDAVGATFQLKESPVAFAKIFGASLAEQLVQRRLLIAYLFLFTSAGTALF
                     LCFRTLRQFFKKQADTAEDKRGRSAGRWNYFLLLWLIVPPLVLYLLSLAWRPCHQPRY
                     TLHASLALYLIYGGAITAISQKYLRRIALAALMLFYGFQQMLVIGEPQHPDWLGAAAH
                     IRKEARGDDLILSFNSMWKRVFTYNLGPVSNVISYGADSEILADQAAFFLQLGILSRD
                     DDKPRDVWIVVLTDYYESGPDQELNRALHRRQLPFTMCEFGGIQHVLLYHVRGGTEAL
                     APYTPHPGLSEEAAEDYTFMATEFWKQQEFTVAAAAAENAARINPEYAQAYALAGMSY
                     KESGNMDKALAAFDRAIALDPDGFPWNHLNRADLLLKKGDYERARVAAEKGLALLPED
                     ALGLILKGRACIGLGLLEEARNLLQAARAKGPDGRLIEEAFQELENAGEAAP"
     gene            complement(14473..15744)
                     /locus_tag="PKN92_07100"
     CDS             complement(14473..15744)
                     /locus_tag="PKN92_07100"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNZ48596.1"
                     /translation="MVDVCSFKALRFNTEITGCPDAVLTPPYDVIDAEARERLGASGP
                     FNMTHLMLPKAQGGRNAYDTAAALLKQWRESGALVCDDTPGIYLLRQKFLDDTGMMRI
                     RKVFFALIRIPEADEKSILGHERTFDKPVEDRLMLTRAVKANLEPIFVMYSDPDQTLT
                     TDLFRVMDVEAPLFAARTSDGVVQELWRSDYPDALQAHLKDQVLYIADGHHRFKTACT
                     YRDEQRAAAGDTGFTGKAYDFVLAGFVAFEDPGLCIFPTHRYLPADFTFGDDELLEKL
                     KTWFDCDRLADEDAPEKALALNPGACRFVLYAGKKKAWLLTLKEDKREALVGTDRHAA
                     WRDLDVAVLHRGIFSHILHLPDSVPYGYGQHAEAVIKEVDEGKASVAFLLRATLSRQV
                     RACAEAFEPMPQKTTFYFPKLPSGAVLNPLE"
     gene            complement(15799..16545)
                     /locus_tag="PKN92_07105"
     CDS             complement(15799..16545)
                     /locus_tag="PKN92_07105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNZ48597.1"
                     /translation="MKTSRINTVAGMAFLLFAAAGLLIVQGTAAAADLETEHNRISYA
                     IGMQLGTMLKQSQLDVNLPSLMEGIGDMLEGKEPALNEEQLQEVEAALQRKMQEAHEA
                     TAKQMEAEAAENLGKSKEYLAEAEKKEGVNKTESGLLYSVDKQGEGKKPTATSKVRVH
                     YRGTLIDGTEFDSSYKRGEPVTFGVNQVIPGWTEALQLMNEGSKYTLIIPPDLAYGPQ
                     GNQRIPGNSVLLFEVELLEVLSDTPEIQIQ"
     gene            complement(16697..18385)
                     /gene="recJ"
                     /locus_tag="PKN92_07110"
     CDS             complement(16697..18385)
                     /gene="recJ"
                     /locus_tag="PKN92_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNZ48598.1"
                     /translation="MTESLYHWQIAAFDHAACAPLARSLGVSPLLVHLLRLRGADTAE
                     KMQDFLHPRLAHLGSPFDLPDMSVAVDRLLLARKHQESVLVFGDYDVDGITATALLTR
                     ALRRFGIARVSSDMPDRFSEGYGLTAARVEKAAEEGHHLIVTVDNGTSAHEAAERAVE
                     LGIDLIVTDHHSLEEKLPPALAVINPKRCTPDHPAYMLAGAGVALKLSIALNGTPNDL
                     DIAALGTVSDIVPLLGENRVIVALGIRHMIQHQRLGIAKLAQAARFHISQVDSQKIGF
                     QLGPRLNAAGRMETGHDALRLLLTEDAQEAAELAEKLNAVNEERRAIETKIYEEAVST
                     LDSFFKEEQRSIVLSRENWHQGVIGIVASRILSGFRRPVIICCMGDDGLLHGSGRGIP
                     GFDMVAALNGCAEYLIRYGGHKAAAGITLAPERLDDFRSAFEEQAALQLGKGKLKPQL
                     ELDALVSLSQIDTAFVTALKNMAPFGQHNQEPIFCASGVDLLPASIQVLREQHLKFAV
                     QQNGVTHNVIGFYKAERFCSASLPRHMDLVFSPFFNTFNGNNAIQLLLHDLRSA"
BASE COUNT         4034 a         4894 c         5152 g         4344 t
ORIGIN      
        1 caacgcactg ttcaccagga aaactaattt acagaaactt cttgacataa cccaataatt
       61 gcatttcagc cttcaatccg cccatctcag aggcttaaaa gttgacagat cgtgtgtacc
      121 gcatctatga tagaatgtta tagagtgcac ttgaaccgaa aggagacgat accatgtcca
      181 gcagtacccg gaattctgaa accaattctc agaccacacg gcgcagcttt ctgcaggcgt
      241 ccaccgttgc agcggcaggt gcggtgctgt ccacagctcc gcgttcttat gctgcgagtg
      301 aaaaacttgc cattcacggc ggtccggcag ccattcccaa tcctgataca gacgcctggc
      361 gctggccgcg ctacggcgaa gtggaagaag aagccctcgc cgccatgctg cgttcgcccg
      421 actacagcga tattgccgca ttggaacagg aatggctgga gcagaccggc ggctcctacg
      481 ccaaggcata ctgcaatggc acaagcgtcc ttttgtccat gtattttgca ctaaacctgc
      541 cgccgggcag tgaagtcatg gtgcccagct ataccttctt tgcgagcatt gtgcccatgc
      601 gccttttcgg gctggttcct gtttttgtgg atatcaatcc gcgaaccctt aattttgatc
      661 tggaagatgc caaaaaaagg cttactccca atacccgtgc tattttgccc gttcactgga
      721 tcgggctgcc ctgtgacatg gatgccattt cggactgggc aaaagaaaaa gggttgatcg
      781 ttctcgaaga ttgcgcccat gcccagtacg ccaaactgaa agggcagcac atgggtacct
      841 tcggcgaaat gtcgattttt tcgtatcaga tgagcaagcc tctgcccggc attgaaggcg
      901 gtatgggatt gtatgcgacg caggaatatc acgaccgcgc caccatcttc ggtcattatg
      961 atacacccgg tattcccgac gacagcccct acaagaaata tcacggtacc ggtctgggca
     1021 ccaagacgcg tatgcacccg ctggcagcaa agcttgtccg cctgcagatg cgcgagctga
     1081 aagagcgtgt cgctgccggt gtggcacaga ccagaagact caatgatgtg ctgacgcagc
     1141 ttcccggtct ctatgagcag acgagcggcc gcaacgatgt ggagcgtctc tattatgagt
     1201 ggaacatgct tttcatcgat gagaaagaag cgggtgtgtc acgtcaggcg gtggtagatg
     1261 ccctgcgcgc ggaaggggtg cgtgccgatg cgctggcata ccggcttcag cacgagcagc
     1321 ccctctataa agatgcggaa tggtggcacc atgcaccggt cattcctgag ctgcccggtt
     1381 ctgcagctgc caatgccacc agcgttgctc tgccctattt caccaccgat gcccctgaaa
     1441 tggtggaagg ctatattcgc gcctttgaaa aagtctgggc acaccgcaaa gacctggcat
     1501 aaccgctttt cgttttaaca agtatggtgc cggtactccg gagagtggcg ttccggcacc
     1561 ttttcactga gcgtcactag tgagtataca aaggaaacaa aagacatgaa agtgatctgc
     1621 aggcgttatg cagtcgtaag tctggtttta acgataacgg cttttgcttt cttcagctgt
     1681 gcctgtctgg cagaaaagac ctgtgcagaa ggcagtcctg agccactccg tcttgcctgc
     1741 cgtgttgcca actacggcaa gtttcaggat caggcgctgg aacatatcag cagtatggga
     1801 cttcgttatg tgtttatgag cattccgaaa gcggaagagg cggaagcggt gctggcgaaa
     1861 ctgaaagccc tgaatcttga ggcgcttgtg gtgcgcggtg ataccaactt atccaccgat
     1921 gcttctgttg atgagcttcg cacccagttt gaaacctgca agcggatggg tgtgcattat
     1981 atgtttctct cccccaagcg ccatgatgct ccgaaagaag atgtttacgc acggctgcgc
     2041 cgggcgggcg atgcggctgc ggataatgat gtgattctgg tgcttgaaac ccatcctgat
     2101 ctgggtacca atgctgctgc acatctcgag acgatgaaag cgatcaatca cccaaatgta
     2161 cgggtgaact ttgacagcgc caatattctg tattacaacg aaggtgccga tacggtggaa
     2221 gagctgaaga agtgtatcga ctatgtcgcc acagtggaag tgaaagatca taacggcgag
     2281 cctgagagct ggttttttcc ggcactgggc gacggtatca tcgacttccc cgctttcttt
     2341 gctgtgctgc gggagcacgg gtataccggg ccggtgacca tcgaagtgga agggattaag
     2401 ggagtggaat ggagcgcggc cgatacctgc gaagccatgg aaaagtctgt gcagtatctg
     2461 aagcgcattg aacacttcaa ataattttcc ggtccggcag agccggattt ttcagcacct
     2521 ttctttttta ttgtaagcgc aacaacaagg aacatgttca accatgagca cagctggcac
     2581 attgcagatg cccgattatt tcatgattat cggctacttt gttcttatgc tggtgatcgg
     2641 tctgtatttc taccgggcta tgcgtgatgt gaaggaatat ttcagcggtg gcaacagcat
     2701 tccctggtgg atgagcggca tctcttttta catgagcagc ttcagcgttg ccgcctttat
     2761 cttctatcct tccctgtgct atcgctacgg ttttgtgggc atcacgcttt tgtgggttgc
     2821 catccccgcc actcttttcg gcgtgctctt tctggcggta aaatggcgcc gtgcccgtat
     2881 ttcaagccct gtggaatacc tggagcagcg ctacagcccg ctgctccgac agatttttgc
     2941 ctggcagggc gtacctgtga aagtgattga tgacggtata aagctctttg ccataggcac
     3001 cttcatgtct gtcagtttga atatggatat tacgacgagc atggtggcag gcggcgtaat
     3061 catgctgatc tatacgttta tgggcggact ctgggcggtg aatgttaccg actttattca
     3121 gttcattatt ctcacggctg ccattatcat cctgctgcct ctttccttct atcacgcagg
     3181 cggctggaac gccgtcagcg aaaacatgcc cgaaggcttc cttaaattta ccaatgctga
     3241 atacggctgg ggctatgtcg cttttctgat tgtcatgtat tctctttcct ggagcagcaa
     3301 caactgggcg ctgatccagc gctactattg tgtacccaaa gaaaaagatg ccatcaaggt
     3361 aggtgttctg gtcattgtcc tgtatctgtt cggtccccct cttatgttca tgcccgccat
     3421 tgcggcgaca cagatttttc ccgaacagct cgccgatgca ggcacagtct atcccatgct
     3481 ctgtaaaacg ctgctgccgg cgggcatgct cgggctggtt gtggcagcta tgtttgcagc
     3541 caccatgtcc acgctcagcg gtgactacaa tgtctgcgcc agtgtgctga cccatgatat
     3601 ctaccagcgc cttttccgcc cgaacgcatc tgaaaaagag ctggtcaatg tggggcgtat
     3661 cgccacgctg ttggtgggtg ttatcgctct gggcacggca ctgctcatgg cacggggcaa
     3721 aggcgaggat cttttccgta tcatggtgac cctctttggc gtggcgacgg caccggttgc
     3781 cattcccatg attatggggc tgctgtcgag acgcgcttcc gcccggggcg cgctcaccgg
     3841 ttttctagcc gggcttacag tcggtctcgt tctttttgcc ttgtcgcggt attcgaagcc
     3901 gcttcagtgg ggcgaggcag cggcctggct tcccgagtcg aaggagctga tggtgctcgg
     3961 ctataaagcc aaactggaaa tggtgctttt tctggcatca ggttttgtaa ccctgctggt
     4021 tatgctggcg gaccggtggc taatttcaat gtctcccgtt gaaaaacagc ggatcgacaa
     4081 tttttatcac cgcctctcca tccccattgg agagatgccg ggcgaggaag atatcgaaac
     4141 acgttctgcc ggcgtgtcgc ctttcggcat tgtcggcatt tccctctttt ttacaggtct
     4201 tctcatgatc tgtgtgctgc cctgggcagg gttgctgatg accgccctcc tcaatggcac
     4261 cattgcggtg atcctgctgg ctgccggggc gattctggcg tggccgaaga aaaaaaacag
     4321 atctaaggct tgatgccgat ccggtaccct ttatcgtcca cgcgatgcag caggatggtg
     4381 atgccctttg tttcaaaata ctcatcggtc gcctttctgg cgccggcgaa agaggcgtag
     4441 tcatcaatca tcagtacacc gccggatacc agtcggggat acaaatgctc cagctcgtgt
     4501 ttcgtggaac tgtaccagtc ggtgtccaga cgaagcagcg caatttgctc cgggatagta
     4561 cccggaatgg tatcctccac tttcccttta atgaaatgaa gctgttcgcg cggatatcct
     4621 gttgaccata aaaggtcgcg cacattgtcg tatttggcaa aaagcgtatc agccgtttct
     4681 ccccgctcca gcgcatccag ccacagctgg tgtgcgggca cgccgtcagc ggtaacatct
     4741 atttcatctc cggcaggcca gccgtcatcg aaagtgtcat agaggaacag ttctcttttg
     4801 tcggcgtgca gtcgctgcag acacagggtc atggcaagag agctgcctcc tttccatacg
     4861 ccgcactcca cgaaggcgcc gggcagcccg gcagccgtta catactcgac agcacgacgc
     4921 agcgagtaaa tgcgctcaag cgacgtcatg gtgtggggtg caacggccgt ccagagatct
     4981 ttatcccagt cgtcaaagtc aatagggaaa aaatctctga tttccggagc ggaaaatttt
     5041 cgaatttcat aacccatttt gcgaagcagc gtgttggcgg cgatcttcaa tggcaaggct
     5101 aaagacggca tgaccgggta tacctccttc cggcaggaaa acgcaggatt gcagaatgac
     5161 agcaggtcag ggatgtaaag actattctac cgaaaacata ccccgggagc ctatttttta
     5221 caaagacgca ctcagtggca tccgggagct tttttaaccg ctgaaatccc atgttgatgc
     5281 ccccgtgatg caaaaggaaa agagcagatg atttctacgg caacctgcaa aacgcttctt
     5341 ccgggtgtcg gcatcgtggc aggcatgctc tttctgttta tacctgcgca ggcttattgc
     5401 gccaatgcgg aaggagtttt tcttctcttc ggacttttct gtatctgcag ttgcctgcct
     5461 gtgcctacgg tgagatgacg ctgtatcctg tgtccgcatc tttgctatac ttgctgtatc
     5521 cgcacatgcg gttgacgggc acctccgggc tgcctgttac cggcgtgttc cctgtttttt
     5581 tccctttatc aagatgctgt tgagcatcgg caaaaggagt tcttttgtga gtgcatctac
     5641 cattcttcag gagctgcggg agatgtcgaa ttacctcggt gcagaacacc ggggctatgc
     5701 cattatcggc gaaggcaata catcggcgcg gattgacaaa gaccgccttt ttgtaaaagc
     5761 gtctggtacc tgtctggcaa caatgggtcc tgctgacttt cttgaagtga gtatttcgaa
     5821 agtgacccgg cttctggact ccaaagaggc caccgatgat gatgtgcggg aaaccttgaa
     5881 atcagcactg accgatccag ccgaaacccg gatgccttcg gtggaaacca tgctccatgc
     5941 gctgctttat gcttatccgg aatacaattt catcgggcat acgcaccccg tttttacgaa
     6001 cagtgtcctt tgttctgtcc atgcggaaga ggcaatgctg ggcaggctct gtccggatca
     6061 tattgttgta atgggacgtg ccaccgtgtt tgttccttat gtggatcccg gtctggtgct
     6121 cgcccgggaa gtacgcgacc gggtgcgagc gtttattgat aaagaaaatc agccgcccaa
     6181 ggcgatcgca ttgcagaacc acgggctttt tgcgcttgga tccaatgccc gggctgtgat
     6241 gaacattacc gatatggcgg aaaaaatgtc gcatattctg gtcggcgcct acagcatggg
     6301 cggtccccgg ttcatggccg ctgccgatat agaccgtatt gacagccgcc ccgatgagaa
     6361 ataccggcag gagcggatcg cctaatcagc cagacgcccg cctgaacttt aaccaagctg
     6421 atctcccagc gcaatgaggt atgaaccatg agtaaacttg acacatacag acaggctcag
     6481 caggcgcttc ctgcaaacta tgaagcctgg caggttttcg gtgccggact ggaaaatgtg
     6541 gggcgggacg ggaagtctgt gactgtgcct atgcgtgcgc ccgcagccaa tgaagtgctg
     6601 ctgcgtatcg atgccctggg actctgcctt tcggatatga aaattatcaa tcagggcggg
     6661 aatcacccgc gtttgcgtgg cagagatctg cagaatgatc ccaccgtgct cggtcatgaa
     6721 tgcgccgcaa ctatcgttgc tgtcggcgag aactggaaag atcagtttca gcccggtcag
     6781 cgttttatcg ttcaggcgga tatctattac aaaggcatcg ggtatgcctt cggctatatg
     6841 attcccggcg ggcttgctga atatgctttt ctggatgagc gcggattggc tggcgacgag
     6901 ggctgctatc tcctgcccgt gaaggagagc accggctttt cagaggcggc gttgagcgag
     6961 ccctgggcgt gtgtggtcat gtcctatgcg ttggacgaaa gggtcacgcc caccggcgcg
     7021 acgcagctcg tggtcgccga tgatcctgca gactgggcgg aaaaaatgcc ccgtgcgcag
     7081 cgggtaccga aaactctgga agggcttgcg gacgatgccc gttttgacga tatcattcta
     7141 gttgcaccta ccccggcgct ggtggaaaca ttgggcgatc gtcttaatcc caacgggatt
     7201 atgttcctcc tgggcgaccc ggcggaagac ggcgatacgc aaatcgatgt gggcgccatt
     7261 cattatcaga ataagcgcta tttgggcggc ggcagcacac tggaggaagt ggcggaagcc
     7321 aaccgccgca ttgatttgct tcccggcggc acgtctctct ttatcggcgc gggcgggccc
     7381 atgggtcaga tgcatgtcca gcgtgccatt gaaatgcgaa acggctcggg ctgtgttgtt
     7441 gtgacggatc tggatcgcgg tcggctgacc catattgaaa aacgtttcgg tgatctggct
     7501 aaagagcgca atgtgaaact gattacgctg gcacccggtg atttcgaaga tcagcaggcg
     7561 atgaatttgc gcatggcggc tctgggcgga gcgaacggtt atgacgatgt ggtggttctt
     7621 gcgcctgtgc ccgctctggt gaaacaggcg gtggaacttt cggcaaaaaa tgcctttgta
     7681 aatctgttcg ccggtctggc gaccggtacc agagcttccg tctctctcag gcagatctgc
     7741 aggggtgtga agatgatcgg gtgcagcgga agccggatta aagatctccg cgatgttctg
     7801 aacatggtgg aagccgggac gctcgacagc aatcgcagtg ttgccgctat cggcgggctc
     7861 gatgcggcgc ataaaggact cgccgctgtt aagaatgcgg tgtacccggg aaaaacagtg
     7921 atttatcccc agattccttc gtttcccctt acagcaattg atgagcttgt cgcaaaggaa
     7981 ccggaaattg cggccgcatt aggcaccggc ggcgtctgga cgaatgaagc cgagaaactt
     8041 ttcctggaga aatatctgcc ctgaccggaa gcagagaggg gctacttgga ccggggcgct
     8101 gttgtgaaaa cggcgccgct cctgaatgca gggaaaagcg gctgcgcagc ggcagtggca
     8161 ccggagagtt cgacggcgtg gagggctgtt gtgccgtccg gtgtctcttc acgaatccgg
     8221 aagtgcagtc ttgccgtctg cccggaggca atggcgcttt gcccgccgta aattgccagt
     8281 gtgaggcgtc ctgcctgggc ctcgtgagcg tctatctgct ttgaggtgcc cgccagtgca
     8341 tcaccgggaa gcacttcctc caacagcagc tgttccgggt cgtaggtgaa ttgaaagaga
     8401 agcacggaag cgtctgccgt cccttttttg aaaacaaccg gtacctcaat ggcagcaccc
     8461 gaccggctga cggcggaacc cagcgcaagc cagcccgcct ctccgccatc cccttcagtt
     8521 cctgtacctg acgtccagca gcgcggtgcc ggacggttcg caaggaaacg ataccaccca
     8581 ccgctctgcc acagcttcgc tgcctgaatg gcatagacca gaggcgtttc ttcctgctgc
     8641 cagctctgca gaaaagagag cgcttcttcc tgtgagattt tccagtcacc gttcttatcg
     8701 gcggggtgaa gcaggctgtc tgctgctgac gctggtgcgg aagtcgccac aagcaaaagg
     8761 aaattcaggg ttgcaataga aaagaaaaca actcgtttat tcataatttt ccttaaacct
     8821 tgtatgaaga aagtatacct tccttttcac gactacgtca aaagagtggg cacacgctca
     8881 aatttgcttc ttttgggctt tctgtggtat ccttcttgta tcggttcttc agtgcgatgg
     8941 tcgcactgac gctaagaggg aatccggtga gagtccggag ctgccccgca gcggtaatca
     9001 ggaacgaacg gcgcaattct gtccgaagcc ttcggcggga taaacgcact ggccccgatg
     9061 ggcggggctg ggaagcggtg ccaagtagaa aagccttctt cattcaaggc aaagcctgta
     9121 agtccgaaga cctgccgata caggaactgt tgtccaacag gtgatgcctt cgcgggaagg
     9181 ctgcacgcgc tttttttgtc cttccctttt gtcctgcctg cgaagcatta ccctgacgac
     9241 gacgcatcac tgaacgaggg ttcgatcatg gtacttcgct ttttttcctc tctctctgcc
     9301 cttctgttac tcctcctttc tcctgtgttt tccgcaggtg cacagagccc tgtcccttat
     9361 aatcaggcga cggtggagac actgtatccc tttccgctga ctatggatgc agcgctgatc
     9421 agctttgact gggatgctac cggggcgctt cattataccg tgggcgatcc caactggggc
     9481 accaaactgg aagtctataa gctgaacggt tccgtacccc aacagatctt cgcttcttcc
     9541 tctgtttggg caggctcccg ccttacccgc ctcggcgctt acatgttttt caatgatggc
     9601 ggtgattttg tccgttcgga tttcaattat tacgggtatt cagcgtcggg ctctgagcct
     9661 gtatttccac tgctggaggc gccttttgag gcgagtctct ggggactgga aacccgcaat
     9721 gaaggggagt ttttcgcttc cggctccgca acaacctgga gaccgggtgc gctcttttac
     9781 ggacagatgg acggcgccgg agcttttagc gcaaggcctc tggtgcaatt cggcgaagtg
     9841 ggcaactctc ccggtccgct ctgctttgat gcgcaaggaa ctctttacta tgtccccgga
     9901 tatgctttcg ccggaggggc ttccatttat cgctggacgg cagaagaagt cgctgacgct
     9961 ctggcagatc ccgtaaccaa gtcacttgcg ccggataatc acctctggaa tacgctgccc
    10021 gccccttatg acggcgccac aggcatggca tgcgacagca acggtaatgt ttatgtgacc
    10081 gctaccgcct ggggttctcc gagtcagttc cttcttttta acgcttccga tgcctctccg
    10141 atactggttg ccgattacgc aggcaggctt gagacgctgc gttatcggga taacagcctt
    10201 gcggtcagct gtgcttccgg gattttttcc attcctctgc tccgtgttga atcggggaca
    10261 ccgtcccagg tgattaccgg gaaagttggc gaaacggcag tgttgtcggt gcagacccgg
    10321 ggcggcattg gtgagcgtca ttaccagtgg tttgagcgca gccagaacaa agcggacatc
    10381 gttcttggcg acgatttgtc ttttcttgct ttgcccatag aagaagaaag cggggacaga
    10441 gcattttact gccaggtttc cgatgcaacc ggtacggtga cctcagagct tttcacgctg
    10501 gtagtcgaaa aaagtgtgcc tgcttatacc gtttatggtt tggcggtggc agtgctgctt
    10561 attctggctg cgggttggat tcttcttcag cggcagcttc tgtgcggtcg gtaatggcag
    10621 tgcggtgcag ctctttttcc agaacaggaa tcagcgcctc gcacacgata tcggctttct
    10681 tttcgatgcc cggattgcgc tgatggcaga tatcgccgaa gagatttgcc gtcccgtcca
    10741 gtttttctgc cagcggaatg taaagaagcc ctcgctcggc gcatagcttt ttgatcgcct
    10801 cgttgtacag cttcaggatg tgcaggtaca tatcaaagga tatatagcgc ccgccccatt
    10861 tcatgcgggt ggtgtactcg taataatcca gttccagggc gttgagccgt tctctgttcg
    10921 gtgcggcaaa actgcataag gcaagagcaa cccctttttc ccgggcgtac gcctccattt
    10981 gccgcaagtt ttcgattttg aagcagtcaa tatcggcagc catttcctgt tctggaggca
    11041 ttatccattt gccaagatac cggattacaa actggctttt ccgcagatac tgctggaagc
    11101 gggtagcgcg attaacccag cccggtatga gctcatggca gatatcattg acggcattgt
    11161 agatgagaat catgtcgggt tccagcgcca gataatcagc catgcgcatt ttatgcttgc
    11221 tcgaattcat gccggagagt ccgcagttga tgacatcgat ctttttcccg ccgaaatgct
    11281 ggttgagccg catttccaga atggcagggt atgtcagttc attgacggga ccttcctctg
    11341 tagtggaggc gccgaggcac agaatgcggt aggaatcttc aggcccgggc acatatatgt
    11401 cggcatcccg gaagccgtag ttattcatat aaaagtgttc ggttatggcg agtacgctga
    11461 tgatatcctg tcgctccacg tgctttttat aaatgaaaaa gggcatttcc cagaggctgt
    11521 cttccggaac agcgcgccag atttctgttt tgggatagat gacccacaca aggggctcgc
    11581 cggtttcttc ctcgaaagta ttttcggaaa aaaggcaata cccgaagggg cctgctgttt
    11641 cgccttctgt tttcgggaaa aagcggattt ctccgcctgt gcgcacctcg tcgaaagcgt
    11701 ccagcaaagg ccgtacagct gcttcgccga ggaatgcgtt gagaggctgt tgctttttta
    11761 cctgaggctc agaaaaggta gcggcaatga taccgtcttt tcggatggca tgaatgtgaa
    11821 actcaaagac gttggcgaac atctgcctgt cttcctgagg catccgcata aaataaagga
    11881 tgcgccgccg catttcttcc tcagccgttg gctccggcag gcgttccatc gacttgcccg
    11941 gtccccgata gttcgccctc agtacttcgt cgtacaggcc gtctgaaaaa tcatcggact
    12001 tatcccggat aataggccac aactctccgg cgacaaggga caggaggaac gggttggttg
    12061 cttcaatctg tttccatcgc caccgcgtcc agccttccag cacaacacag aaacagatca
    12121 tccatagcag gagggtcaac aaaaaaaata cggttctgcc tgcccatttt ttcatggcgc
    12181 tgcttccccg gcgttttcaa gctcctgaaa agcttcctca attaaccgac cgtcaggccc
    12241 ttttgcacgg gcagcctgga gcaggtttcg tgcttcctca agcagaccca ggccgatgca
    12301 tgcccgtcct ttcaggatga gccccagcgc gtcttcaggc agcagcgcca gccctttttc
    12361 agcagcgaca cgcgcccttt catagtctcc tttcttcaaa aggaggtcag cgcggttcag
    12421 gtggttccag gggaagccgt ccgggtcgag cgcaatagcc cggtcaaaag cggcgagcgc
    12481 tttatccata ttgccgcttt ccttatagga catgccggca agggcatacg cctgcgcata
    12541 ctccggattg atgcgtgcgg cattttccgc agcagcagcg gcaacagtga attcctgctg
    12601 tttccagaat tctgtcgcca taaaggtgta atcctcggcc gcttcttccg aaagcccggg
    12661 gtgcggggta taaggcgcaa gcgcttcagt gccgcctctg acatggtaaa ggaggacatg
    12721 ctgtattccg ccgaactcac acatggtaaa aggcagctgt cgacggtgca gcgcacgatt
    12781 cagctcctga tccggcccgg attcgtaata atcggttaac acaacaatcc acacatcgcg
    12841 aggcttgtca tcgtcccggg agaggatacc gagttgtaag aaaaatgctg cctggtcggc
    12901 aagtatctct gaatcagcac cgtaggaaat cacatttgat acaggtccca ggttgtacgt
    12961 gaatacccgt ttccacatgg agttgaatga gaggatgagg tcgtctcctc gtgcttcttt
    13021 tctgatgtgt gccgccgcac ccagccaatc gggatgctgc ggctcgccga tgaccagcat
    13081 ctgctgaaaa ccgtaaaaaa gcatcagtgc agcgagggcg atacgacgca gatacttttg
    13141 tgaaattgct gtgatggcgc cgccgtaaat aagatacagt gccagcgatg catggagcgt
    13201 gtaccggggc tgatggcagg ggcgccacgc aagagagagc aggtatagca cgaggggcgg
    13261 cacgatgagc cagagcagga gaaaataatt ccaccgcccg gcactgcgcc cccttttatc
    13321 ttcggcggtg tccgcctgct ttttgaaaaa ctgcctcagc gtgcggaaac acaaaaataa
    13381 agcagtaccg gccgaggtaa agaggaacag ataagcgatc aacaggcgac gctgaacgag
    13441 ttgttctgcg agactggcac caaagatctt tgcaaaagca acaggacttt ctttcagctg
    13501 aaaagtcgca ccgaccgcat cgtcggcgaa aatatcagcg gcaacttcca tcacggtagg
    13561 aagccgcagc cactggtcgg tgctgtcctg tccccagaac tggatggtgg cgacatagta
    13621 gacggcgggc attacagcca ggcataccgc aagagaccac agggtgaggc gcacgatatc
    13681 acggggcctt gtcagcaacc agaagagccc ctgcacaaaa agcagcagga gagcaaaggg
    13741 atgcgtccag gagagcaggc aagttgccgt gagaccgagg aaccaccagc gttttttccc
    13801 ctcttccaaa gccctgatga aagcgtatac cgtaataagt gcgagcagtg ccagaagccc
    13861 gtacatgcgg atttctttgg cgaactgcac gtggatgggg gaaagactga ggcatgccat
    13921 cgcaatgagc cctgcccggc ggtcgtagag cctgcttccg atcaggtaaa tcaaaggaag
    13981 cagcagcagc cccacacaaa cagacaaaat acgcaaagcc gcaggcgagg catgaacgta
    14041 ccggttccag gcaaactcga gaaccagata caggggcatt gcggcgggat ccaccagctg
    14101 gttctgctgg aggaactgct ttagcgtggg cctatccagc cgctccacct gctggttcca
    14161 ctgtcggtaa tgaggcgact gcgtccaggg ctcgggtgga tccagaaagg cgacggtggc
    14221 gaattcatcc caccagacac tttccgcagt gagatgctgg aagcgtatca ctgcacccag
    14281 aagcaaaatc agcataaagc agagggcagt tgttccccgg gcgggatggg ttgaatcagg
    14341 agatatcagc tgactgctgg agttagcgtt tgccataata gggtttgcgc cggacttcct
    14401 gttctcgttt tcccgcctga agagaccggg actgatctct tcaggcagga gttcgaaaac
    14461 gctatacaga ttttattcca aagggttcag cactgctccg gagggcagtt tcggaaaata
    14521 gaaagtggtc ttttgcggca taggctcaaa ggcttccgca caggcacgta cctgccggga
    14581 cagggtggca cggagcagaa aagcgacgga agctttccct tcgtccacct ctttgatgac
    14641 ggcttcagcg tgctggccgt agccgtaagg gacgctgtcg ggcagatgca gaatatggct
    14701 gaaaatcccg cgatgcagca cggcaacgtc gagatcacgc caggcggcat gccgatcagt
    14761 gccgaccagt gcttcccgtt tatcttcctt cagcgtcagc agccacgcct tctttttacc
    14821 ggcatacaga acaaagcggc atgcgcccgg gttgagtgcc agcgcctttt ccggtgcatc
    14881 ttcgtccgca agccggtcgc agtcaaacca ggtcttcagt ttttcgagga gctcatcatc
    14941 tccaaaagtg aagtcggcag gcagatacct gtgcgtcggg aaaatacaca gtcctggatc
    15001 ttcaaaggca acaaagcctg caagaacgaa gtcgtaggct tttccggtaa accctgtgtc
    15061 gcccgcagcg gcacgctgct cgtccctgta ggtgcaggct gttttgaagc gatggtgccc
    15121 gtcggcaata taaagcacct gatctttcag gtgcgcctgg agggcatccg gatagtcgct
    15181 gcgccacagt tcctgcacga caccgtcgga ggttcgggcg gcaaacagag gcgcttcgac
    15241 gtccataacc cggaagagat cagtggtaag cgtctgatcc gggtcggagt acatgacaaa
    15301 gataggctcc agattggcct tgacggcacg ggtcagcatc agccggtctt ccacaggctt
    15361 gtcaaaggtg cgttcatgcc cgagaatgct tttttcgtcc gcttcaggga tgcgaatgag
    15421 cgcaaaaaag accttgcgga tgcgcatcat gccggtgtca tccagaaatt tctgccggag
    15481 cagatagatg ccgggcgtat catcacagac aagtgcgccg gattcacgcc actgcttgag
    15541 cagcgccgcc gcagtatcat aggcatttct gccgccctgc gctttgggca gcatgagatg
    15601 ggtcatatta aacgggcctg aggcgcccaa ccgctcccgt gcctccgcat cgatgacatc
    15661 atagggtggt gtcaggacgg catcggggca gccggtgatt tccgtattga agcggagtgc
    15721 cttaaaactg cagacatcga ccatgagccg gaatctccaa tgtttaaaag acgactctag
    15781 tgacagggtg gcaaaaactt attggatctg aatttccggc gtgtcagaaa gcacttccag
    15841 cagttccact tcaaaaagga gaacggaatt gcccgggatg cgctggttgc cctgaggacc
    15901 gtaagcgaga tccggaggaa tgatcagcgt atatttggaa ccttcgttca tcagctgcag
    15961 cgcttctgtc cagccgggaa tgacctgatt cacgccgaag gtgacaggct caccccgttt
    16021 ataggagcta tcaaattcag tgccgtcaat aagcgtgccc cggtaatgta cccgcacttt
    16081 ggaagtggca gtcggcttct tgccttcgcc ctgcttatca acagaataga gcagcccgga
    16141 ttcggtcttg ttgacccctt cttttttttc tgcttcagca agatattctt ttgatttgcc
    16201 gaggttttcc gctgcttccg cttccatctg cttcgcagtt gcttcgtggg cttcctgcat
    16261 tttccgctgc aatgctgctt caacttcctg cagctgctcc tcgttcaaag caggctcttt
    16321 gccttccagc atgtcgccga ttccttccat cagcgaggga agattgacat cgagctggct
    16381 ttgtttaagc atggtaccca gctgcatgcc gatggcatag ctgatgcggt tgtgttcggt
    16441 ttcaagatct gccgccgcag cagtgccctg aacgatcaac agaccggcgg cagcaaaaag
    16501 aagaaatgcc attccggcaa cagtgttgat acgggaggtt ttcatggata cgttcctttt
    16561 cagtttagag ttgttatgct gactacgctg ccgcaggagc agacagcgca tgcagtagtc
    16621 gggagggtat tgtgagacac tgctaatacg gtaccgtttt cttaggggca ttcgcaactt
    16681 ttaccacgaa aaaacatcag gcggagcgca ggtcgtgcag cagcagctga atggcgttat
    16741 tgccgttgaa ggtgttgaag aagggagaga aaacaagatc catgtgcctt ggaagtgatg
    16801 cgctgcagaa acgttccgct ttataaaaac cgatgacgtt gtgtgtaaca ccattttgct
    16861 gtacggcaaa tttcaggtgc tgttctctga gcacctgtat agatgcgggc agcagatcca
    16921 ctcccgatgc gcagaaaata ggttcctggt tgtgctgtcc gaagggtgcc atgtttttca
    16981 gggcagtcac aaaagcggtg tcgatctggc tgagggagac caaagcatcc agttcgagct
    17041 ggggctttaa ttttcccttg cccagctgca gtgcagcctg ctcttcgaag gcactgcgga
    17101 agtcgtcgag ccgctccggt gccagggtaa tgcctgccgc cgctttatgc ccgccgtaac
    17161 ggatcaggta ctctgcgcag ccattcagcg ccgccaccat gtcaaagccg gggatacccc
    17221 gtccggagcc gtgcagcagg ccatcatcgc ccatacagca gatgatcacg ggccggcgaa
    17281 agccggacag aatgcgtgag gcgacaatac cgatgacacc ctgatgccag ttttctcttg
    17341 aaaggacaat agagcgctgc tcttccttga agaaagaatc gagggtactg acggcctctt
    17401 cgtagatttt agtctcgatg gcacggcgtt cttcattaac agcattcaat ttttcagcga
    17461 gttccgccgc ttcctgggca tcttccgtga ggagcaggcg caaggcatca tgaccggttt
    17521 ccattcttcc ggcggcattc agtctgggac ccagctgaaa gccgattttc tgggagtcga
    17581 cctggctgat gtgaaagcgg gcggcctgtg ccagtttggc aatgccaagc cgctgatgct
    17641 gaatcatgtg ccggataccc agagccacaa tcactctgtt ttcgcccagc agcggcacga
    17701 tatcgctcac ggtacccaga gcggcgatgt ccagatcgtt gggcgttcca ttaagtgcta
    17761 tggacagttt cagggcgaca cctgcgcccg caagcatata ggcgggatga tccggtgtgc
    17821 agcgtttggg attgataacg gcaagtgccg gcggcagctt ttcttccaga ctgtgatgat
    17881 ccgtgacgat gagatcgata cccagttcaa cagcacgttc cgctgcttcg tgggcgcttg
    17941 ttccgttatc caccgtgacg atgaggtgat gcccttcttc cgccgctttt tcaactcggg
    18001 cggcagtcag cccgtatcct tctgaaaaac gatccggcat atcgctgctg acccgggcga
    18061 taccgaagcg gcgcagggcg cgggtgagca gcgccgttgc cgtaataccg tccacgtcat
    18121 aatcaccgaa aaccagcacc gattcctggt gctttcgtgc cagcagcagg cgatcgacgg
    18181 cgacggacat atcgggcaga tcaaaaggag agccgagatg tgcgagtcgc ggatgcagaa
    18241 aatcctgcat tttctctgct gtgtcggcgc cgcgcagccg cagaaggtga acgagcagcg
    18301 gacttacgcc caggctccgg gcaagaggcg cacaggcagc atggtcaaag gccgcaatct
    18361 gccagtggta aagggactct gtcattgcgc cagccgttca cgggcaaggt tgacaatggt
    18421 gtcg
//
LOCUS       DAOPWB010000059        18382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3078_length_18382_cov_5.702625, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000059 DAOPWB010000000
VERSION     DAOPWB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 18382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18382
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3078_length_18382_cov_5.702625"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PKN92_07115"
     CDS             complement(<1..1120)
                     /locus_tag="PKN92_07115"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNZ48599.1"
                     /translation="MRKYLLFIAVLPGLLILHPGCHLIQPEARFTISPETGEAPLFVT
                     LNNQSAPGLFGAPRYEWDFGDGSALSTEESPRHLYEKAGQYSVTLTLKTLFQKHRATR
                     RLEVREPGSGEIVTVNFEVRPREGAAPLVVQFTDTSFVAGAAVTGRIWDFGDGTHIRA
                     LNPVHRYTDAGSYDVTLTIFTENSTYSHTMENAVSVAKTEQRLSATRQLSPGSGTDEN
                     NQIAVTLHFAGFIREVPTPVLLKEVLPEGWRFAGLLQNTTPEALLTEEEVGQVQFFWV
                     DCPPLPFSLSYLIEAPEPCTEGILSGQLHMRESGSVEMQHPGETRVVCEALPLEGESE
                     GEISEGELPGEGEIEGEISEGEVPGEGEIEGEISEGEVP"
     gene            complement(1321..2934)
                     /locus_tag="PKN92_07120"
     CDS             complement(1321..2934)
                     /locus_tag="PKN92_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNZ48600.1"
                     /translation="MSQTAAETKDRIDVHNPKTGEVLYTVSEYTVEEIADIYEKARAA
                     QAVLAKMSVAERLQETQKLKKYLCDNMDEVCRKIISETGKSRTDALLAEIYPILDMLD
                     YYAKNAVRFLADQKVNTPIVMLGPGLTGTVRDFFSKKAKIIYEPLGTVLIVAPWNYPF
                     HLSIFPAISAFLAGNAVIIKPSTQTPLKGLYEEMIEKSGFMKDAIQCVYGSRKTGERL
                     IDAKPAKIFFTGSVSGGRHVMTQAAKYLIPVELELGGKDPMIVFDDVHLERATSGALW
                     GGMLNCGQTCTSVERIFVQESILDAFVAQLKEKMEKISTLSTKPDDDGGLCVGCMTTS
                     FQIEEIEAQLQEAQEKGADIVSGGKCVPGTHIFPPTLVVTRNLSLKILNDETFGPVVT
                     VVPFKTEEEAIRLANDSPFGLNSSVWSRDLVRAERVARALVSGNVSINNVLATQANAA
                     LPFGGAKESGMGRYHGPFGLHSFSNIKSMTIDQSSDRSEINWYPYTKEKFELLTKLFK
                     TLYSGHPLALLRAGLLGSKLAALVKKQRL"
     gene            3165..4118
                     /locus_tag="PKN92_07125"
     CDS             3165..4118
                     /locus_tag="PKN92_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48601.1"
                     /translation="MKKIYAGLLLPVLLLTYNAFSAPPEIVKLGTIDLDLVETTPIVF
                     KERLYRFEYVRHNYWDNSSGKSFFRFIDHETGEATPSFAAGYHFGSATVDGDLVIVTG
                     TKAVDGEEVDLFVSRDLEHWDSWNALTLPGYGLFNTSLCKTPEGYTLMFEVGKPEEVA
                     GVRFTARFATSPDLKVWTLTAPECTFDKSRYTAPHCLRWHEGWYYNFYLEEISGSYEQ
                     YVVRSRDLIHWDPSPVNPVLKADDSDRTIANQALTAEQRKQIAEAVNLNNSDFDYCEF
                     EGHLVINYSWGNQRGVEFLAEARYEGSESAFLRALFPPAGN"
     gene            4140..5891
                     /locus_tag="PKN92_07130"
     CDS             4140..5891
                     /locus_tag="PKN92_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNZ48602.1"
                     /translation="MKNNSDRPLFQPLPMSRRDMEAYGWSEIDILLVTGDAYIDHPSF
                     GTALIGRVLVSEGYRVGVVAQPRWDTTDDICAMGRPRLFAGVTSGALDSMLAHYTAFR
                     KLRHGDAFTPGNRHGARPNRASLVYTGLLRQAFSGLPVVLGGVEASMRRASHYDFWSD
                     KLRRSILLDSKADLLVYGMGERAVLEIARRLSSLPAGSPDQQAVLRGIPGTVFSLTGR
                     TKESAADLIPSPDKICTLPSHEDILASPAALMTATLTMEEVLHQGRKWMVQLSGAQEV
                     YFTPPAAPMNEEEMDRIYALPFTRRPHPVYKERIPAADMIQFSITAHRGCAAGCTFCS
                     ITLHQGRQIQSRSRNSIVAEAASLTKHPDWKGSISDVGGPTANMWGAYCADDPSSCRR
                     PDCLYPRRCPHFKCDEAGLVGMLQEVAALEGVRHLRVSSGVRYDMSRENTLFMRTLIS
                     DFVGGQLKVAPEHSEDRVLALMRKPSLSRFEEFLALFRRESQRAGKEQYVVPYLISAF
                     PGCTAGDMRKLAAWLKERNWSPQQLQCFIPTPGTVATAMFYAGIDTKMRPIPVARTDR
                     ERIIQHQILMPGGGKKK"
     gene            6045..7112
                     /locus_tag="PKN92_07135"
     CDS             6045..7112
                     /locus_tag="PKN92_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ48603.1"
                     /translation="MLSYCDPGAMKTRILEVLERYPYRAPWWMKNTHAQTIWVGTVRK
                     KTGLLLAPKFRRERWDTPDGDFLDLVFVDGKENAPVVVLFHGLEGDAHSYYISGYGRL
                     FKKLGWTFAAMLFRSCGFEMNRTAKFYHIGATEDPKFVLEHFRERYPDRPIFGIGISL
                     GGNVLGKLLGEEGENARLWMDGAFLLSPPYEPQTAAPYFHQLFNGFYARHFIKTMKPK
                     ALDLAWRFPGLLDEDAIRNSDDFYTFDSAVTAPLNGFVDAEDYWKKTGCGQFLPGVRV
                     PTLLMTALDDFFILPECMPYEAVEQSPWLYPLFTERGGHGAFVCGPLPFLTRSWYEEQ
                     SLRFFRMLLKEGVGGKTSGQA"
     gene            complement(7124..8062)
                     /locus_tag="PKN92_07140"
     CDS             complement(7124..8062)
                     /locus_tag="PKN92_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HNZ48604.1"
                     /translation="MILTVTPNPCIDKTLFIRQLTAGAKIRVPRYSCITGGKGCNVSR
                     AVRAMGGETNALFFAAGHTGKHAVEMLEQDDGVPCLPVWVSGMTRTITTVLEEPLHRQ
                     TALFEPGPPVNREEYQAFLQHFREHACRAALVTLNGAASNAGQDTLYFDLIQIAHACD
                     VPVILDAYGAIFAEALKARPYMVKPNLEEMEALTGKRLPTRREQWEAVDFLLAQGITL
                     VVLSLGDQGALVASGDWHCHLLPPAIQEVNPVGSGDALVAGFALGLTQGRSLEDTARL
                     GIAMGTANAMTWDIGVFHPEEVAALQSRITITPRQP"
     gene            8181..10010
                     /locus_tag="PKN92_07145"
     CDS             8181..10010
                     /locus_tag="PKN92_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HNZ48605.1"
                     /translation="MRRKTGIHAMIKPYVPPELYENASSEVEEPLDYSSLISAPPQPV
                     HIPAPHDFESLNRDAPLRPPVVVLRSQWADYFIDFFTPVFIFFACQSVLVYLLMIRYV
                     YTDTHDSNLRFFLFFLLLGIVALNRLLAHASFQTKSGYALLLGYSVAAYVLATSMGFG
                     VYFTGQSVAASEGQGSRFVQFFGPHLWPFILNSSLLIFVWWVVNRLTRECCLDEDAFA
                     GDIGILTAAADRLTAILNRPEKEKTVSAKIAAPAGISEAWQDLRPRDPTETYVPLTPQ
                     SRYKTEELRPGSHPGMAIFYFSAPVLLLFILGPRYFQHLGEKGLEAGSFYVVFYLFCA
                     FFLLALTALRQLRAFFSVRSVSMSEELMWFWLVTAFCIIVVILRAAALFPMPSLPPPA
                     YVAFHEQDPYRQGAPLVTLLPVEVPAFQSWDQQTFRKYLDGALLVFFSLVLLYVAGKR
                     VLSALERHMLQQERSIPLFTGLLRLLMRFLAWLMGVSVAKLMQRGLRIQRHIAFSTAY
                     GNPYRLGGNTDISVRDHTVYAYEALRALSTDIGSPVQDSMTPYEFLEHFPKRLDSLKE
                     EASDVIRLYVIAQYSTLEMDARMEDRLRRFWQAFQIVRDANIR"
     gene            10029..10823
                     /locus_tag="PKN92_07150"
     CDS             10029..10823
                     /locus_tag="PKN92_07150"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNZ48606.1"
                     /translation="MKIPAAVRPLYLLWQVWHHPLLNLLLPMYCKICHGRLLTDENLY
                     FCGECWESSPRIERPFCTFCGKPHQALSGLGERSNFPCADCRKKPNSFVRRIWGAAQY
                     SEAVARGIRLFKFYGKEPMAEPLAALMLAFAEKEMDAEGYDCIVPVPLYPSRERERGY
                     NQSRLLAEHLLHFFARCTLDESLCRIRPTRAQSTLKSVDRAENVRGAFAVKGDALKGK
                     RVLLIDDVITTGGTVTECARALRRAGVQEVDAFAPALAFASLRLDR"
     gene            10830..11216
                     /locus_tag="PKN92_07155"
     CDS             10830..11216
                     /locus_tag="PKN92_07155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNZ48607.1"
                     /translation="MKTFFSFVKNRLISLFCALKGLHVLLKTQTHARLHLLATVFVLV
                     LSGYFRIAAAEWGLVLFAVGFVWAAEAFNTALEKLADAAVPARNPLVGQAKDAAAAAV
                     LIAAVIAGIIGLIIFLPRLLPVLFAN"
     gene            11340..12167
                     /locus_tag="PKN92_07160"
     CDS             11340..12167
                     /locus_tag="PKN92_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48608.1"
                     /translation="MADNKGTTDRRFPAGFLPGMLIFACFFSISFTGYSDLLTLQGGE
                     TLTGTLLRIRSGTLVFRTSLEGQMMLPLDRARSLSTVNAFSLKMSDGRMLIGRFSQEE
                     QQQLLLPPDPALPVFLILEEIAEALPIPATTQVQPTETPGLPRLQSDSIPRLQTPGRE
                     KAPDAFGTENDNVLRLPGLTEKLRDPLPSGEGQHPFSMTEEPALPPPLLRAEKTELPP
                     PMLYRELRPRLLADSPDTPLLPLENLFKNPDAFFSLPFSDTGSKLRIHYRPERAPSD"
     gene            12270..14333
                     /gene="glgX"
                     /locus_tag="PKN92_07165"
     CDS             12270..14333
                     /gene="glgX"
                     /locus_tag="PKN92_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNZ48609.1"
                     /translation="MKPELNAADYQVIPGRPYPSGPVVYDQGVRFTIFSRHAHRVWIA
                     LFDSVEAREPYWEYEFDPRRHRVGDMWSVFVSGVKEGAYFLYRMDGPWAPEKGHRFRS
                     DVYLLDPYAKAFVGDIHQGTMKCVVVNDRMDWHDDRRPGIPMKDLVIYEAHVKGFTRH
                     ASSGVSAPGTYRGFIEQIPHLQALGVNAVELLPVQEFGETLLGSCSMQTGQELVNYWG
                     YSNIGFFAPSCRYAAQANCFEHLDEFRELVRALHDAGMEIILDVVFNHTSEGDERGPT
                     LSFKGIDNVVYYLLDHGSYLNYSGCGNTVNCNHPLVKDFILDCLRYWVAVMHIDGFRF
                     DLASILGRDQQGNIREDAPLIARIAEDPVLRGTKLIAEAWDAGGAYQVGHFGGVRWAE
                     WNGKYRDDVRRFWRGDAGQKGVFATRLLGSADLYQHNGRSPEHSINFITCHDGFTLHD
                     LVSYNRKHNEANGEGNRDGADDNFSWNSGIEGETDDPAVLDLRRRAKKSLVATLFLSL
                     GVPMMLGGDEMGRTQKGNNNAYCQDNGISWFDWSQLEANGEIFQFYKSMIAFRYENKA
                     LRRNRYYLGVSMVPHADTPDLAWYEADGSSADWHQEEGILACRIYPCENQGIGLYLCF
                     NATNRPVRFVLPAGAWRLRIDSSACGGLDYFERATAPAVDQAVVEVSGKSVVVLESPV
                     MGISR"
     gene            complement(14323..15225)
                     /locus_tag="PKN92_07170"
     CDS             complement(14323..15225)
                     /locus_tag="PKN92_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48610.1"
                     /translation="MALTEKQELYISRYREEVRENLKGHLTAPLLEQALSHLRLSILE
                     EILKHAGQQPAEDLQVLQALKELGSPAERAQVLIRLYRAQMSAPESSQRYAAPAARQV
                     PAEQKEAAAAKKEADAEKVVWLGVCLHIARTASLPAWLIRCAAVILGLFGAPLMLIVY
                     MGAFFFFRFQGVLETKEQVHLFRCAGHLFITAFLIILFYCAGKYGLEGIAWAHQYFLK
                     QPLPDLAEWGWLASQQQALLFWALFLLLPPALLSALPVPSGWALSLKRAAQAGVTLYA
                     VLIAFGLASSIAGILLQFYSEFTG"
     gene            complement(15285..16751)
                     /locus_tag="PKN92_07175"
     CDS             complement(15285..16751)
                     /locus_tag="PKN92_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNZ48611.1"
                     /translation="MAKNHYSALSRRRFLGTCGTTAAAALLSVHAAKGAEHTGSFRAG
                     IAVADMTPPVGTSMAGHFTDRMNTGAHDPIQARCLVLDNGAHRLALVTVDNCLVPRAP
                     FDAAKARIQKETGIPPAYVITSATHTHTGPTATPVFMSDPVPGYCDFMAEKIAEGVNE
                     AVSKLEPAQIAFGCGSVPGEVFNRRWFVQPDSIPENPFGEKTDRVRMNPPQADASLIE
                     PSGPTDPAVPFIALRRPDGSPLAVYANYALHYVGGTSGLELSADYFACFAESLKKMLV
                     PEESGKPFLALLSNGASGNINNINFREPGKSYEPYEKMQMVADNVAKEVFDRYQELQF
                     VDHVPLGAAVRDLTLGVRRPTEDEVIRARRILEEAGDQPLRAAKEIYARETLFMADYP
                     DTVSIPLQALRIGDVGIAAIPCEVFVEIGLAIKADSPLPLTFPVALANAYHGYLPTAE
                     HHALGGYETWRARSSYLEVAAAEHIRKASLELLEKVKA"
     gene            complement(16775..18085)
                     /locus_tag="PKN92_07180"
     CDS             complement(16775..18085)
                     /locus_tag="PKN92_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ48612.1"
                     /translation="MRVRPRWISIFAPVVLLLYSGALFAASTVEPADRLDQPWWAERH
                     EAVLKQIKEQPVDLILMGDSINHGWEGAGKEVFDKYYKNRNTVNMGFSGDRTEHVLWR
                     LDHGEVDGIQPKVVSLMIGTNNCSANTAEEIGEGIIAVVKKLREKLPQTKVLLLAIFP
                     REEKPGETRDKLAKASEIASAVADGDMVHYLDIGSVFLSEDGTLTKEVMPDALHPNAH
                     GYALWAAAVEQKLAELLGEPVPPEGFRVLFNGKDLRGWKGLVGDPRSRAKMSEKVLAK
                     RQEKADALMHEHWKVEDGVIVFDGKGENLCTERDYGDFEFIVDWKIQEKADSGIYLRG
                     SPQVQIWDAGLNPEGSGGLWNNQNNPNKPDKCADKPLGEWNRFHIIMVGEKVSVYLND
                     ELVVNNVTLENYWERDKPIYPTGSIELQNHGDKLWFKNIFVKEL"
BASE COUNT         3839 a         4843 c         5163 g         4537 t
ORIGIN      
        1 cgggcacttc gccttcgctg atttcgcctt cgatctcacc ctcaccgggc acttcgcctt
       61 cgctgatttc gccttcgatc tcaccctcac cgggcaattc gccctcgctg atttcgccct
      121 cgctttcacc ttccaacggg agtgcttcgc acacaaccct tgtttctccg ggatgctgca
      181 tttcaaccga gccggactcc cgcatatgga gctgtccgct gaggatacct tcggtgcagg
      241 gttcgggcgc ttcaatcagg taggagaggc tgaaagggag gggcgggcag tcaacccaga
      301 agaactgcac ttgtcctact tcttcttcag tgagtagcgc ttctggagtt gtattctgaa
      361 gtaaaccggc aaagcgccac ccttcgggca gtacttcttt cagcagaaca ggtgttggca
      421 cttcccggat gaatccggca aaatgcagcg tcactgcaat ttggttattc tcgtccgtgc
      481 cggagccggg gctgagttgt ctggtggcag agagacgctg ctctgttttg gcaacagaga
      541 cagcattttc cattgtatgg ctgtacgttg aattttccgt aaaaatggtc agtgtgacat
      601 cgtaactgcc tgcatcggtg taacggtgta caggattcaa tgcccggatg tgtgttccgt
      661 cgccgaagtc ccagatccgc cctgtgacgg cagctcctgc cacaaaagac gtatctgtga
      721 attgtaccac cagcggcgcc gcaccttccc ggggtctgac ttcaaagtta accgtgacta
      781 tttcgccgga gccgggctcg cggacttcca gccttcttgt tgccctatgc ttctggaaaa
      841 gggttttcag tgtcagggta acggaatact gccctgcttt ttcgtaaaga tggcgtggag
      901 attcttcggt gctgagggcg cttccatctc cgaaatccca ctcataacgg ggtgcgccaa
      961 aaaggccggg ggcagactgg ttattcagcg tgacaaaaag cggtgcttcg cctgtttccg
     1021 ggcttatggt aaagcgtgct tcaggctgga tcaggtgaca gccagggtga aggatcagca
     1081 atccgggaag cacggctatg aagagaaggt attttctcat gaactgttcc tttcaaacac
     1141 ctaagtatac aggcccggac gcaaaattca caaatccgga tccggaaagg agaccgccgg
     1201 agacgcgggg tatgggtgct tatccggagg tgtttcgtgc gcacataaat cccgcagccg
     1261 tacacgtctg acggacatgc acagggtgat cagggtgcac ttcgatctgc tgatgaagga
     1321 tcagagacgt tgctttttga ccagagccgc cagtttgctt cccagcagtc cggcacggag
     1381 caacgcaaga ggatgtcctg aataaagggt tttaaacaat tttgtcagca gttcaaattt
     1441 ctcttttgta taaggatacc agttgatttc gcttctatca ctgctctgat ctatagtcat
     1501 cgatttgatg ttgctgaagc tgtgcagtcc gaaaggaccg tgataacggc ccatgccgct
     1561 ttcttttgca ccgccgaagg gcagggcggc atttgcctgt gttgccagaa cgttgttgat
     1621 ggagacattg cctgacacga gcgcacgggc tacccgctcc gcccgcacca gatcacggga
     1681 ccatacactt gaattcaggc cgaagggaga atcgttggcg agccggatgg cttcttcttc
     1741 agttttaaag ggaacaacgg taacgacagg cccgaaggtt tcgtcgttca ggattttcag
     1801 ggaaagatta cgggttacca ccagtgtagg cggaaaaata tgggtgccgg ggacacattt
     1861 gccgcccgag acgatatcag ctcctttttc ctgtgcttcc tgcagctgtg cctcgatttc
     1921 ttcgatctgg aaagaagtgg tcatgcagcc cacacaaaga ccgccgtcat cgtccggctt
     1981 ggtgcttaaa gtgctgattt tttccatttt ctccttgagc tgcgccacaa aagcatctag
     2041 aatgctttcc tgcacaaaaa tccgctccac agaggtgcag gtctggccac aattgagcat
     2101 accgccccac agggccccgc tggtcgcccg ttccaggtga acatcgtcaa agacgatcat
     2161 gggatctttt ccgcccagtt ccagttccac agggatcaga tatttggcag cttgtgtcat
     2221 gacatggcga ccgcctgaaa cactgccggt gaaaaagatt ttggcaggct tcgcatcgat
     2281 cagccgttct ccggttttgc ggctgccgta tacgcattga atggcatctt tcatgaagcc
     2341 gcttttttcg atcatttcct catacagccc tttcagcggg gtctgtgtcg agggcttgat
     2401 aatgacggcg tttccggcga gaaaagcgct gattgcgggg aagatgctca gatggaaagg
     2461 atagttccag ggcgccacga tcagtactgt gccgaggggt tcataaataa tttttgcttt
     2521 tttgctgaag aaatcccgga cagttcctgt cagtcccggc cccagcatca cgatgggcgt
     2581 attgaccttt tgatcggcga gaaagcgaac ggcatttttc gcataataat caagcatatc
     2641 cagaatagga taaatttccg caagaagggc atcagtgcgg ctttttcccg tctcactgat
     2701 gattttacgg catacttcgt ccatgttgtc gcagagatat tttttcagtt tctgcgtttc
     2761 ctgcagacgt tcggcaacgg acatttttgc cagaacagcc tgagcagccc gtgccttttc
     2821 gtaaatatcg gcgatttctt caacggtgta ttcagaaacg gtgtaaagta cttcccctgt
     2881 tttcgggttg tggacgtcga tgcggtcttt tgtttcagcg gcagtctgtg acatggcttc
     2941 ctgctccttt caaaggtaac atgtgatgac agaagtaaac agtcacgcaa aggtgcatat
     3001 ccggcctgtc tcaaagggat gccggaacag ctttgacctg ctgcatggta ccatacttcc
     3061 agcggataca ccaaaaactg aacaaagttc actgtactct gccggttgtg tcctcatgaa
     3121 atattgctgt acaatagccg ctggatgaat atggaaacag gactatgaaa aaaatctatg
     3181 ccgggctgct tcttcctgta ctgctgctca cgtacaatgc tttttccgcc ccaccggaaa
     3241 tcgtcaaact gggcaccatt gatcttgatc ttgtggagac gacacccatt gttttcaaag
     3301 agcggcttta tcgtttcgaa tacgtccgcc acaactactg ggataacagc agcggcaagt
     3361 cttttttccg cttcattgac catgagactg gcgaggcaac gccttctttt gccgcgggat
     3421 accatttcgg cagtgccacc gtggatgggg atcttgtgat tgttacggga acaaaagcgg
     3481 tggacggaga agaagtggac ctctttgtct cgcgcgatct ggagcactgg gacagctgga
     3541 atgccctgac acttcccggg tacggacttt tcaatacctc gctctgcaaa acaccggagg
     3601 gctatacgct gatgtttgaa gtgggcaagc ccgaagaggt ggcaggggtt cgttttacgg
     3661 cgaggtttgc cacgtcacct gatctgaaag tgtggacgct taccgcgccg gaatgtactt
     3721 ttgataaaag caggtatact gctccgcatt gcctgcgctg gcacgaaggc tggtattaca
     3781 acttttatct ggaggaaata tccggatcct atgaacagta tgtggtgcga tcccgcgatc
     3841 tgattcactg ggatccgagt ccggtgaatc ctgtgctgaa agccgacgac agcgaccgca
     3901 caatcgccaa ccaggctctg acagcggaac agcgtaaaca gattgcggag gcagtgaatc
     3961 tcaataactc ggattttgac tactgtgaat ttgaggggca tctggtcatc aattattcct
     4021 ggggcaatca gcggggtgtt gaatttcttg ccgaggcgcg ttatgagggt tcagagtcgg
     4081 cgtttctccg tgcgctcttt cctcctgccg ggaattagac aaattcttac tggaaattca
     4141 tgaaaaataa ttcagaccgc ccgctgtttc agccccttcc catgtcgcgc cgggacatgg
     4201 aagcgtacgg ctggagcgag attgacatac ttctggtcac cggcgacgcc tacattgatc
     4261 atccttcttt cggtacggca ctgatcggca gggttcttgt ttcagaagga tacagagtgg
     4321 gagtcgtggc gcagccccgc tgggacacaa cggacgatat ctgtgcgatg ggacggccgc
     4381 gcctttttgc cggagtgact tccggggcgc tggattccat gcttgcccat tatacggcct
     4441 tccgcaaact gcggcatggc gacgctttta ccccgggcaa ccgccatggc gcccgcccga
     4501 atcgggcttc tctggtgtac acaggtctgc tgcgtcaggc tttttccggg cttcctgttg
     4561 tgctgggggg tgtggaagcg tccatgcgcc gtgcttctca ttacgatttc tggtcggaca
     4621 aactgcgccg ttccattctt cttgacagca aggcggatct tcttgtgtac ggcatggggg
     4681 agagagcagt gctcgagatt gcccgccgtc tttcctccct tccggcaggc agcccggatc
     4741 agcaggctgt cctgcgcggc attcccggca ctgttttttc actgacaggc cgcacaaaag
     4801 aaagtgcggc agatctgatt ccgtcgccgg acaagatttg tacgcttccg tcgcacgaag
     4861 atattcttgc atcgcctgcc gcactcatga cagctacgct cacaatggag gaagtgcttc
     4921 atcaggggag aaaatggatg gtgcagctgt ccggcgcaca ggaagtgtat ttcacaccgc
     4981 ctgccgcacc tatgaacgaa gaagaaatgg acaggatcta tgcgcttcca ttcacccgcc
     5041 gcccgcatcc cgtatataag gagcgtattc ctgccgccga tatgatccag ttcagcatta
     5101 cggcacaccg cggctgtgcc gccggttgta ccttctgctc cattacactg catcaggggc
     5161 gacagattca gtcgcggagt cggaattcca ttgtggcgga agccgcatcg cttacgaagc
     5221 atccggactg gaagggaagt atttccgacg taggcggccc gaccgccaat atgtggggcg
     5281 cttattgtgc cgatgatccc tcgtcctgcc gccgtccgga ctgtctttat cccaggcgct
     5341 gtccgcattt caaatgtgat gaggcgggtc ttgtcggcat gcttcaggaa gtggcggcac
     5401 tggagggcgt gcgtcatctg cgcgtgtcaa gcggtgtgcg ttatgatatg agccgggaaa
     5461 acactctgtt tatgcgtacg ctcatctccg attttgtggg tggtcagctg aaagtggcgc
     5521 ctgagcacag tgaagaccgt gtgctggcac tgatgcgcaa gccttccctg agccgttttg
     5581 aggaatttct tgcccttttt cgccgggagt cgcagcgtgc cggaaaagaa cagtacgtgg
     5641 ttccgtatct gatcagcgcc ttcccaggat gcaccgccgg cgatatgcgc aagctggctg
     5701 cctggctcaa agaacggaac tggagccccc agcagctcca atgtttcatt cctacgcccg
     5761 gcaccgttgc caccgccatg ttctatgcgg gaatagacac gaaaatgcgg cctattcctg
     5821 tggcacggac agaccgagag cgtataatcc agcatcagat ccttatgccg gggggcggaa
     5881 aaaagaagta aattttccgg tatcggcact gccggaacag aaggctgccc tgagctgctt
     5941 tcctctggaa aatccggtgt taaatgcgtt agagtaatcc ctgtgggttg atcaaggggt
     6001 gctcatggca ccccgactga tgtcgtcttt aaggattatt gctcatgctg tcgtactgtg
     6061 atccgggggc aatgaaaacg cgcatactgg aggtgcttga acgctatcct taccgggcgc
     6121 cctggtggat gaagaacacc catgcgcaga cgatctgggt ggggacggtc cgcaagaaga
     6181 ccggtcttct tctggcgccc aagttccgaa gagagcgttg ggatacaccg gacggagact
     6241 tcctggatct tgtgttcgtg gacgggaagg agaatgcgcc ggtcgtagtg ctttttcacg
     6301 gcctggaagg agatgcgcat tcttactaca tatccggcta cggccgcctt ttcaagaagc
     6361 tcggctggac ttttgccgcc atgctgttcc gttcctgcgg ctttgaaatg aaccgcaccg
     6421 caaagttcta tcacataggt gccacggaag atccgaaatt tgttctggaa catttccggg
     6481 agcgctatcc tgatcgtccc attttcggta tcggcatatc actgggcgga aatgttctgg
     6541 gcaaactgct gggcgaagaa ggagaaaacg cacgcctgtg gatggacggg gcttttctcc
     6601 tgtcgccccc ttacgaaccg cagacggcgg caccttattt tcatcagctc ttcaacggtt
     6661 tttatgcgcg ccactttata aagaccatga aacccaaagc gctcgatctt gcgtggcgtt
     6721 tccccggtct gctggatgaa gacgccatcc gtaattcaga tgatttctat acttttgaca
     6781 gtgctgtcac ggcgccgctg aacggttttg tcgatgcgga agactactgg aagaaaacag
     6841 gctgcggtca atttctgccc ggggttcggg tacctacgct gctcatgacg gctcttgatg
     6901 atttttttat tctccctgaa tgcatgccct atgaagcggt tgagcagtcg ccgtggctgt
     6961 atcctctttt tacggagcgg ggcgggcatg gggcttttgt ctgcggaccg ctgcctttcc
     7021 tgaccagatc gtggtatgaa gagcaaagcc tccgcttttt ccggatgctg ctgaaggaag
     7081 gcgtcggagg aaagacgtcc gggcaggcat aagcagccgc actttacggt tgacggggcg
     7141 tgatcgttat ccggctttgc agcgcagcca cttcttccgg atggaagacg ccgatatccc
     7201 aggtcatggc attggcggta cccatggcaa tgcccagccg ggcagtgtct tcgagggatc
     7261 tgccctgtgt aagcccgaga gcgaagcccg caaccagagc gtcaccggag cctacagggt
     7321 tcacttcttg aatggcaggc ggaagcagat ggcagtgcca gtcccctgaa gcaaccagag
     7381 cgccctgatc tcccagagac agtactacca gcgtgatgcc ctgcgccagc agaaaatcca
     7441 cggcttccca ttgttcccgt cttgtcggaa gacgttttcc tgtgagcgct tccatttctt
     7501 cgagattggg cttcaccata taaggacgcg cttttagcgc ttctgcaaaa atagcgccgt
     7561 aggcatccag aatgaccggt acatcacagg catgggcaat ctggatgaga tcaaagtaca
     7621 acgtgtcctg tcccgcattg gaggcggcac cattcagcgt aaccagtgct gcacggcatg
     7681 cgtgttcgcg gaaatgctgc aaaaaagcct gatattcttc ccggttcacc ggcggacccg
     7741 gttcaaaaag agcggtctgg cggtgcaagg gctcttccag cacggtcgtg atggtgcggg
     7801 tcattccgga aacccagacc ggcaggcagg gaacaccgtc atcctgttcc agcatttcca
     7861 cggcatgctt tcccgtatga cccgctgcaa aaaacagggc attggtttcg ccgcccattg
     7921 cccggacagc acgggacacg ttgcagccct tgccgccggt gatgcagctg taccggggca
     7981 cgcggatttt tgcgcctgca gtaagctgcc ggatgaaaag ggttttgtcg atgcatgggt
     8041 tgggcgtaac cgtaagaatc ataagaggcg gctctttcta tagcggaaaa ataaaggaca
     8101 cggcatcatt ataaaagccg gacgggaagt aaagaaagtt gcggcacttc tttttctgtc
     8161 ctgtcatggg atgggctata atgagaagga agacagggat tcatgccatg atcaagccct
     8221 atgtgccgcc ggagctctat gaaaacgcct cttcggaagt ggaagagccg ctggactaca
     8281 gctctctcat atccgcaccg ccgcaaccgg tgcatattcc ggcgccccat gactttgaaa
     8341 gtttgaaccg tgatgcgcct ctgcgtcccc cagttgtggt gctgcgcagt caatgggcag
     8401 attattttat tgactttttt acgccggtct tcatattttt tgcctgccag tccgttctgg
     8461 tttatctgct gatgatccgt tatgtctaca cggataccca tgacagcaat ctccggttct
     8521 tcctcttttt cctgctgctg ggcattgttg ccctgaaccg gcttctggcg catgcttcct
     8581 ttcagacaaa gtcaggctac gccctccttc tgggatattc cgtagcggcc tatgtgctgg
     8641 ctaccagtat gggcttcggt gtttatttta ccggacagag cgttgcggca agcgaggggc
     8701 agggcagccg tttcgttcaa tttttcgggc ctcatctgtg gccttttatc ctgaacagca
     8761 gtcttctgat tttcgtgtgg tgggttgtga accggctcac ccgggaatgt tgtctggacg
     8821 aagatgcctt tgcgggggac atcgggattt taacagccgc cgctgatcgc ctgacagcta
     8881 ttctgaaccg tccggaaaaa gagaagaccg tttcagcaaa aattgccgca ccggcgggta
     8941 tctcggaagc gtggcaggat ctgcgtcccc gtgatccgac agagacctat gtgcctttga
     9001 ccccgcagag ccgatacaaa actgaagagc tccgtcccgg aagccatccg ggcatggcta
     9061 tcttttattt ttccgcaccg gtgctgctgc tttttattct gggcccccgg tattttcagc
     9121 acctcggaga gaagggactg gaagcaggca gtttttatgt ggttttctac cttttctgcg
     9181 ccttttttct gttggcactg acagcactgc gccagctgcg tgcctttttc agcgtacgca
     9241 gtgtttccat gtcagaggaa ctgatgtggt tctggctggt gacggcgttc tgtattattg
     9301 tggtgattct gcgggcagcg gctctttttc ccatgccgtc actgccgcct ccggcctatg
     9361 tggcttttca tgagcaggac ccgtatcgtc agggcgcccc gcttgtaacg ctgcttcctg
     9421 ttgaagtgcc ggcgtttcaa tcctgggatc agcaaacgtt cagaaaatat ctggacggag
     9481 cgctgctcgt tttttttagt cttgtactgc tgtatgttgc cgggaaacgg gtcttatccg
     9541 ctctggaacg tcatatgctg cagcaggagc gttccatacc gctgtttacg gggctgctgc
     9601 gactgctcat gcgtttcctt gcgtggctga tgggggtttc cgtggcaaaa ctgatgcaaa
     9661 ggggactgcg cattcagcgg catattgcat tcagtaccgc ctatggcaat ccgtatcggc
     9721 tgggcggcaa tacggacatt tctgttcgtg atcacaccgt gtatgcctat gaggcacttc
     9781 gtgctttatc gacagatatc ggttcgcctg tgcaggacag catgacgccc tatgagtttc
     9841 tggaacattt cccgaagcgc ctggacagct tgaaagaaga ggcttccgat gtgatccgcc
     9901 tttatgtcat tgcgcagtat tcgactctgg agatggacgc acgcatggaa gacagactcc
     9961 gtcgcttctg gcaggcattt cagatcgtgc gtgatgccaa catccgataa gtgcagggag
    10021 acaagggcat gaaaattccc gctgctgtcc ggccgctgta tctgctgtgg caggtctggc
    10081 accacccgtt gctgaacctt ctgctgccca tgtactgcaa gatctgccat gggcggctgc
    10141 tgacagacga aaatctctat ttctgcggcg agtgctggga atcgagtccc cgtattgaga
    10201 ggcccttctg caccttttgc ggcaagccgc atcaggcatt gtcggggctg ggggagcgct
    10261 cgaattttcc ctgcgcagac tgccggaaaa aacccaattc ttttgtccgg cgtatatggg
    10321 gagcggcaca atacagtgag gccgttgccc ggggcattcg tctgtttaaa ttttacggga
    10381 aagaaccgat ggcggagcct ctggcagcgc tgatgctagc ttttgcggaa aaagagatgg
    10441 atgccgaagg gtatgactgt atcgtgccgg ttccgctgta tccctcccgg gaacgggagc
    10501 gcgggtacaa tcaatcccgg cttctggcag aacaccttct tcactttttc gcccggtgta
    10561 ccctggatga gtcgctgtgc cggatccgtc cgacccgggc gcaaagcacc ttgaagtctg
    10621 tcgaccgggc ggagaatgtg cgcggtgcct ttgccgtgaa aggcgatgca ctgaagggga
    10681 aacgggtgct gctgatcgat gacgtgatca caacaggcgg tacagtgaca gaatgcgccc
    10741 gggcactccg ccgtgccggt gtgcaggagg tggatgcttt cgcgcctgct ctcgcctttg
    10801 cttctctccg cctcgacaga taggacgcca tgaagacttt cttttctttt gtaaaaaacc
    10861 gtctgatcag cctgttttgt gctttgaagg gtcttcatgt gcttttaaaa acgcagaccc
    10921 acgcacggct tcacctgctg gcaactgttt ttgtgcttgt cctttccggg tatttccgca
    10981 ttgctgccgc ggagtggggg ctggtgcttt ttgctgtcgg tttcgtctgg gcagccgaag
    11041 cattcaacac agcgttggaa aagctggcgg acgccgctgt tccagcccgg aatcctctgg
    11101 tcggtcaggc gaaagatgct gctgcagcgg ctgtgctgat tgccgctgtc attgccggca
    11161 tcatcggcct gattatattt ctgccccgcc ttctccctgt tctttttgca aattgagaaa
    11221 aaaagggggg ggttccgcgg ttttgtgtgt ctccccggca tcaggtaaaa taggggaaag
    11281 gcagtcggtt ttggctcgaa agcacccgtg ccgggcagta ttgccgctga ttggacagaa
    11341 tggcggacaa caaaggtaca acagacaggc gatttcccgc aggcttcctg ccgggcatgc
    11401 tgatctttgc ctgctttttc agtatttctt tcaccgggta cagtgacctg ctgacacttc
    11461 agggcggtga aacgcttacg ggtacacttt tgcgtatacg cagcggtacg ctggttttcc
    11521 gtacttctct ggaagggcag atgatgcttc cgctggatcg ggcccggagt ctgagcacag
    11581 taaatgcctt ttccctgaaa atgtcggacg gtcgcatgct gatcgggcgt ttcagtcagg
    11641 aagagcagca gcagctgctg cttccgccgg atcctgcgct gccagttttt ctcattctcg
    11701 aagagattgc ggaggccttg cccattccgg cgaccacaca ggtgcagccg acagagacac
    11761 cggggctgcc ccgtctgcag tccgacagta ttccgcgcct gcagacaccg gggagagaaa
    11821 aggcgccgga cgctttcggc acagaaaatg acaatgtact gcgcctgcct ggtctgacag
    11881 agaagctgcg cgatcctctt ccgtccggag aagggcagca tccgttttca atgactgaag
    11941 aaccggcgct tccgccgccg ctgctgcgtg cggaaaaaac ggaattgcca ccgccgatgc
    12001 tgtacagaga actgagaccg cgccttctag ccgattctcc ggataccccg ctgcttcctc
    12061 tggagaatct ctttaaaaat ccggatgcgt ttttttctct gcctttcagc gatacaggtt
    12121 ccaagctgcg gatccactac cgcccggaaa gggccccttc cgattgacat gcacaaaaag
    12181 ttgcgctccg gaagtatgtt tcagtgttgc atgttattgc tagagcagat aacgattccg
    12241 taatcattga ggagaaatct ttttttcata tgaaaccaga actaaatgca gccgactatc
    12301 aggtgattcc cggcaggccc tacccttccg gtccggtggt ctatgatcag ggtgtccgtt
    12361 ttaccatttt cagccgtcat gcgcatcggg tatggatagc tctttttgat tcggtggaag
    12421 cccgcgaacc ttattgggaa tatgagtttg acccgcgcag acatcgtgtc ggcgacatgt
    12481 ggtccgtttt tgtttccggt gtgaaagaag gcgcttattt tctgtaccgc atggatggac
    12541 cctgggcgcc cgaaaaggga catcgcttca gatcggatgt atatctgctg gatccctatg
    12601 ccaaagcttt tgtaggggat attcatcagg gtaccatgaa gtgtgttgtt gtcaatgacc
    12661 ggatggattg gcatgacgac cggcgccccg gcattccgat gaaagacctg gttatatatg
    12721 aggcgcatgt caaaggcttt acccgtcacg cttcttcagg ggtttctgca ccgggcacct
    12781 accgcggatt catagagcag attcctcatc tccaggcact gggtgttaat gcggtggagc
    12841 tgctgcctgt tcaggaattc ggagaaacgc tgctgggcag ctgcagcatg cagacgggtc
    12901 aggagctggt gaattactgg ggctacagca acatcggttt ttttgcgccc agctgccgtt
    12961 atgcggctca ggcgaactgt ttcgagcatc tggatgagtt cagagagctg gttcgtgccc
    13021 ttcacgatgc ggggatggaa attattctgg atgtggtgtt caaccacacg tcggaaggag
    13081 acgagcgggg accgacgttg tcgtttaaag gcattgacaa tgtagtctat tacctgcttg
    13141 accacggttc ttatctgaat tattcagggt gcggcaatac ggtgaactgt aaccatccgc
    13201 tggtgaagga ttttattctg gactgcctgc gttattgggt cgcagtgatg catattgacg
    13261 gattccgctt cgacctggct tccattcttg gcagagatca gcagggcaat attcgtgagg
    13321 atgcgccgct gatcgcacgt attgccgagg atcccgtgct gcgcggtacc aaactgattg
    13381 cggaggcgtg ggatgcgggc ggtgcctatc aggtgggaca tttcggaggc gtgcgctggg
    13441 cggaatggaa cggaaaatac cgggacgatg tgcggcgttt ctggcggggc gatgcggggc
    13501 agaaaggcgt ttttgccacc cggcttctgg gcagcgccga tctgtatcag cataacggca
    13561 gaagtcccga gcacagcatt aactttatta cctgtcacga tggatttacc ctccatgatc
    13621 tggtgagcta caacaggaag cacaatgaag cgaatggcga aggcaaccgg gacggggcag
    13681 acgacaactt cagctggaat agcggcattg aaggggaaac ggatgatcct gctgtgcttg
    13741 atctgcggcg ccgtgccaaa aaaagcctcg ttgcaaccct ctttttgtcg ctgggtgtgc
    13801 ctatgatgct cggaggtgat gaaatgggtc gtacacaaaa ggggaacaac aacgcttact
    13861 gtcaggacaa tggaatttcc tggtttgact ggtcgcagct ggaggcaaac ggcgagattt
    13921 ttcaattcta caagtccatg atcgctttcc ggtatgaaaa caaggcgctg cgccgcaacc
    13981 ggtattatct gggtgtttcc atggtgcccc atgctgacac gcccgatctg gcgtggtatg
    14041 aagcagatgg aagcagtgcc gattggcatc aggaagaggg tattcttgcc tgtcgtattt
    14101 acccctgtga aaatcagggt atagggcttt acctctgttt caatgccacg aaccgcccgg
    14161 taagattcgt tctgccggca ggggcgtggc ggctccgcat tgacagcagt gcctgcggcg
    14221 gactggatta tttcgagcgt gccacggcac cggctgtgga tcaggctgtt gtggaggtgt
    14281 ccggcaaaag cgttgttgtg ctggagtctc cggttatggg aatcagccgg taaactcact
    14341 gtaaaactgc agcagaattc cggcaatgct ggacgccagt ccgaaagcga tcagtacggc
    14401 atagagtgtg acgcctgcct gcgcagccct tttgagactg agcgcccatc cggaaggaac
    14461 gggcagtgcg ctcaggagtg ccggaggcag cagcaggaag agtgcccaga agagaagcgc
    14521 ctgttgttga gaagcgagcc agccccactc cgccagatcg ggcaacggct gctttaaaaa
    14581 gtactgatgc gcccaggcga tgccctccag accgtatttt ccggcacagt agaaaagaat
    14641 gatcaggaat gcggtgatga aaagatgccc ggcgcagcgg aaaagatgca cctgctcttt
    14701 cgtttccagt acgccctgaa atcggaaaaa gaaaaaggcg cccatgtaga cgatgagcat
    14761 cagcggtgcg ccaaagagcc ccaatataac ggcggcgcag cgtatgagcc acgcagggag
    14821 tgaagcggtg cgggcaatgt gaaggcatac gcccagccag acaacttttt ccgcatctgc
    14881 ttcttttttg gcggccgccg cttctttctg ttctgcggga acctgacggg ctgcaggagc
    14941 ggcgtatcgc tggctgcttt ccggtgcgga catctgcgct ctatacagcc ggatcagcac
    15001 ttgtgcccgc tcagcagggc tgcccagttc tttcaatgcc tgcaggacct gcagatcttc
    15061 agcgggctgt tgcccggcgt gtttcagtat ttcttccaga atactcagac gcagatggga
    15121 cagtgcctgc tccaggagcg gtgcagtcag gtgacctttc agattttcac ggacttcttc
    15181 cctgtaccgt gaaatataca gctcctgttt ttcggtcagc gccataccgc ctccttcttc
    15241 gctgcagcct gatagcgatc cgtttcccta ttgtacgatc ctgttcacgc ttttactttt
    15301 tcaagcaatt caagagaagc cttccggata tgctctgccg ccgccacttc cagataactc
    15361 gaacgggcgc gccaggtttc atatccgccc agggcatgat gttccgctgt ggggagatac
    15421 ccgtgatagg cattggcgag cgcaacggga aaagtcaggg gcagcgggga atccgccttg
    15481 attgccagcc cgatttcaac aaacacttcg cagggaatag cggcgatacc gacatcaccg
    15541 atgcggagcg cctgaagggg gatggacact gtgtccggat agtccgccat aaaaagcgtt
    15601 tcccgggcat agatttcttt ggcggctctc aaaggctgat cgcctgcttc ttccagaatc
    15661 cggcgggcgc gaattacttc gtcttcggtc gggcggcgca cacccagtgt cagatcccgg
    15721 acggcggcac ccagcggaac gtgatcgacg aattgcagtt cctgatagcg gtcgaatact
    15781 tctttcgcca cattgtccgc caccatctgc atcttctcat aaggttcata gcttttgccg
    15841 ggttcccgga aattgatatt gttgatattg ccgctggcac cgtttgaaag cagtgccagg
    15901 aagggtttcc cgctttcttc gggcaccagc atttttttca atgactcggc aaagcaggcg
    15961 aaataatcgg ctgataattc cagtccgctg gtgccgccca cgtagtggag ggcgtaattg
    16021 gcatagaccg caaggggtga accgtcgggg cggcgcagcg cgataaaagg cacagcagga
    16081 tcggtaggcc cggagggttc aatcagcgat gcatcggcct gaggcggatt catccggaca
    16141 cggtctgttt tttcgccgaa gggattttcc ggaatgctgt ccggctggac aaaccagcgg
    16201 cggttgaaca cttcaccggg taccgatccg catccgaagg cgatctgcgc aggctccagt
    16261 tttgacacgg cttcgttaac gccttcagcg atcttttccg ccataaaatc gcagtaaccc
    16321 ggcaccggat cgctcatgaa aacaggcgtc gccgtcggac cggtgtgggt atgggtggca
    16381 ctggtaatga catatgcagg cgggatgccg gtttcttttt gtatccgcgc tttggcggca
    16441 tcgaaagggg cccggggcac cagacagtta tcgacagtga caagcgccag gcggtgtgcg
    16501 ccgttgtcca gcacaagaca gcgtgcctga atggggtcgt gtgcgcctgt attcatgcgg
    16561 tcggtaaaat gcccagccat ggaggttcca accggcgggg tcatatcggc aacggcaatg
    16621 cctgcccgga aagaccctgt gtgctccgca ccttttgcgg catgcacgga cagaagggcg
    16681 gctgccgccg ttgtaccgca ggtgcccaga aatctccgtc gcgacagggc ggagtagtgg
    16741 tttttggcca tgatgtgctt ccttgcgtcc tgtgttaaag ctctttcaca aaaatatttt
    16801 tgaaccagag cttgtcgccg tggttctgca gttcaatgga acctgtcgga tagatgggct
    16861 tgtctctttc ccagtaattt tccagcgtca cattgttgac caccagctcg tcattcagat
    16921 agacagaaac tttttcgccg accatgatga tgtggaagcg attccattca cccagcggtt
    16981 tgtcagcaca tttatccggt ttattgggat tgttctggtt gttccagagt ccgccggagc
    17041 cttccggatt caatcctgca tcccagattt gcacctgagg tgatccgcgc agataaatgc
    17101 cgctgtccgc tttttcctga attttccagt caacaataaa ttcaaagtcg ccgtagtccc
    17161 gctctgtgca gaggttttcg cctttgccgt caaatacgat aacgccgtct tccactttcc
    17221 agtgttcatg catcagagca tcggcttttt cctgacgctt cgccaacact ttttctgaca
    17281 tttttgcacg ggagcggggg tcgcccacaa gacctttcca tccgcgaaga tcttttccat
    17341 taaaaagcac gcggaagcct tcaggcggta ccggctcgcc cagcagctcc gccagtttct
    17401 gctccacggc ggctgcccaa agcgcatagc catgggcgtt gggatggagt gcatcgggca
    17461 taacctcttt ggtcagcgtt ccgtcttcgc tcagaaaaac agagccgata tcgagatagt
    17521 gaaccatgtc gccatcggca acggcagatg cgatttcact tgcctttgcc agtttgtccc
    17581 gggtttcgcc gggcttttct tctctcggga agatggcaag aagcagcact ttcgtttggg
    17641 gcagtttctc gcggagtttt ttcacaacgg ctatgatgcc ttcgccgatt tcttcagctg
    17701 tattggcgct gcagttgttg gttccgatca tgagtgacac gactttgggc tggatgccgt
    17761 ccacttcacc gtgatcaagg cgccacagca catgttctgt acggtcgccg gaaaatccca
    17821 tgtttacggt attccggttt ttataatact tgtcgaaaac ttctttgccc gcgccttccc
    17881 agccatggtt gatggaatcg cccataagga tcagatctac gggctgttct ttaatctgct
    17941 tcaggacggc ttcatggcgt tccgcccacc acggctgatc caggcggtct gccggttcca
    18001 cggtcgatgc tgcaaaaaga gccccgctgt aaagaagaag aactacggga gcaaaaatac
    18061 tgatccatcg gggacgaaca cgcatgatat aaccctttct gtttgaacac gctgcagtaa
    18121 agattatcgg tcggattatt ttttctttgg cgcggccggt atcggtctgc cgtttgccgg
    18181 aacaaaagta cattccgctt attataaagt aacagtatgc catgaacaac agtgagggtg
    18241 gcggggaaaa ttccatgctg cttttgtatg ttagctccgc gcccgccgtc attcacgctt
    18301 ttctccgtta gctcctcccc tttcgtcatt cctgcctccc tccgtcattc ctgcgaaggc
    18361 aggaatctta atcagggtat gg
//
LOCUS       DAOPWB010000060        18345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3089_length_18345_cov_7.437835, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000060 DAOPWB010000000
VERSION     DAOPWB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3089_length_18345_cov_7.437835"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PKN92_07185"
     CDS             <1..626
                     /locus_tag="PKN92_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48613.1"
                     /translation="GNHDHLDDYDKGHKALAQARHSLYGGTSKNGGHYVTRIKDRSDR
                     ILFELFCLNTEERGVGEGIKNFLRQMLSQEQSDQKRPMRLGAFHIPLRQYRDVWENGN
                     AFGVKGEEICFEEEDGSALPLLREAGIQAVICGHDHTNDYSGFAEGIELIYGRASGYN
                     GYGNQTLKKGAKLYRIDPATAALHWESYLPDGTRWHPGPTERRSIAP"
     gene            complement(678..1862)
                     /locus_tag="PKN92_07190"
     CDS             complement(678..1862)
                     /locus_tag="PKN92_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48614.1"
                     /translation="MFFINRRSFLYASAAASLSPFATWAAQEKKQTYRACVIGDSQRG
                     RYGHGFHLIWGLRNDVELVALADPDEEGARKHGEEAGAKRIYTDYREMLEKEKPDLVS
                     VAPRWTTCHQEYLEACAAIGAHGIMEKPLTPDLTGGDAAVAALEAKNLKWAAGFNFRA
                     APGIFHAKRSIINERLIGSVLEVRARGKEDHRSGGEDLIVLGIHLFDLMIYFLGKPLW
                     CEAEILTEGRPALPADVHEATEGLGPIVGDTIFAQFGFKNGRRGYFTSVKGASNDADR
                     YGMDIYGTKGAASIRVDRDPIISYLPEPGWSSCGTEEVWQALPDMPVAPLRSPGAVSQ
                     YAPIIDDLMDAIVQDRHPAVSIQDALAATEMIQAVFASHVAGRRVAFPLQERNHPLAT
                     WH"
     gene            complement(1982..4579)
                     /gene="clpB"
                     /locus_tag="PKN92_07195"
     CDS             complement(1982..4579)
                     /gene="clpB"
                     /locus_tag="PKN92_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNZ48615.1"
                     /translation="MRMDKLTIKSQEALAEALELASNRGNPEITPLHFLRTLAGQEQG
                     AVGSILQRIGVAPAQILTDTDRALDSLPRISGGGQPGLSRDARAVFDAAWKQAQYLKD
                     EYLSTEHILLGILEKGGEAAALLKRAGMDTASVLQALKDVRGTQRVTDPNAEATYEAL
                     QKFSRDLTQLARAGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGTGKTAIVEGL
                     AQRIIAGDIPEGLKNKQVASLDIAALVAGAKFRGEFEERLKAVLSAVQEAEGQIILFI
                     DEMHTIVGAGASEGSMDASNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQP
                     VFVDQPNVENTIAILRGLKERYEVHHGVRIQDDALVAAAALSHRYIADRFLPDKAIDL
                     IDEAASRLRIEIDSMPYEIDVIARRLRQLEIEHLALKKEKDKSAQERLAALEKEMSNL
                     KEDLSAKTAQWKTEKDCIETIRSIQSKIDEARRQEEIAERQGQLDKVAEIRYGTLNEL
                     QKQLAGQNARLADIQKNGSFLSEEVTEEHVSKIVSSWTGIPVDRLMESEMQKLISLEK
                     EIGQRVIGQSDGIKAVSEAVRRARAGLKEPDRPIGSFIFLGPTGVGKTELARALAESM
                     FDDENAMVRIDMSEYMEKHAVSRLIGAPPGYVGYEEGGQLTEAVRRRPYSVILLDEIE
                     KAHPDVFNILLQLLDDGRLTDNQGRTVDFRNTVVIMTSNIGSTVFNESDNTDAEKSDA
                     VRALLRAHFRPEFLNRIDETVVFHPLSRDNIRAIVDVQLKRLAERLESRRLTLDLDDA
                     AKELLAERGFDPAYGARPLKRAIQQYLLDPVAYKIIAGDIKEGDHILVRVDSGELSFQ
                     AVPQEKADS"
     gene            4777..5670
                     /locus_tag="PKN92_07200"
     CDS             4777..5670
                     /locus_tag="PKN92_07200"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ48616.1"
                     /translation="MSMKRKLSIFAAIVAGTLFLAASCLFYLLFHRSPGQFFEQDGTR
                     LFYSDTGRGEAVILVHGIAAQGDLNWRRPGVTRMLAKQFRVITLDMRGHGLSDRPEDA
                     EHYGIHLVTDIIGLMDHLSIDKAHLAGYSLGGFVALKAVTQYPDRFLSAAICSAGWIN
                     PESPGEIPNPYQAPVKNAEIKTSHASVMMPVASKSVFHRIRSGVGDMLIDRKIKKALK
                     ASYPELGVLREDVETIKTPLLCIIGTKDGFLHLARALCQFECPLECIEIGGATHFSAP
                     FKSEFKKNLLLFFQKNSPRHK"
     gene            5733..6182
                     /locus_tag="PKN92_07205"
     CDS             5733..6182
                     /locus_tag="PKN92_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48617.1"
                     /translation="MEVRSDKSLWNQEPSFSEEPLSEEELSDFSFSLLHFSLQSFLSA
                     AFSLEHSPLTGQAGAAAAATGQDALFAQADLQQLPALAEFPSSATAIIEKAATIITTA
                     KTETMLTTRIFFFMFSFSLVYANNLLFSMLIIQTTQTWFLFAKTVYL"
     gene            6326..7783
                     /locus_tag="PKN92_07210"
     CDS             6326..7783
                     /locus_tag="PKN92_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48618.1"
                     /translation="MPPITRRQFLQSATLLAGSALLTHPLKVSARVIGANDRLRIAVA
                     GLNGRGQAHISGWLEQNNVEIAYLIDPEARVLARALKAFEKKAGGHPVPKGVADIREA
                     LNDPELDAISIATPNHWHSLMTIWGAQAGKHVYVEKPMSHDIGEGRVVVAAQKKYGVV
                     IQHGTQARSNLHTAGLHEAIHAGKFGRLKIAYGYCCKPRASIKFAEPAEPPEYLDWNL
                     WCGPAKVEHYHRNYVPYNWHWFWNTGNGDLNNQGTHQLDVARWAIDPDQTHPVRAMAI
                     GGRFQWNDQGETPNTMFSLAEYPNGQMVFFNVRNVNYSGYENQVENEYYFEDGGKIIR
                     DKYYPKGSSQGEPIDVPDGKVTPGGNWASFITACRAGDPSLANGNAQESHYSCVLGHL
                     INNSYRLGVKAPFNVKSGNFGDNKEAYEHFARLHEIMSKGVGIPENGNEYTVGPWLTF
                     DPETETHTGAFSTEATALLKDANRAGFEIPAAAEV"
     gene            7897..9165
                     /locus_tag="PKN92_07215"
     CDS             7897..9165
                     /locus_tag="PKN92_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HNZ48619.1"
                     /translation="MSSSSTLDLAVVLPCLNEGANLRLLLPRIQTVLADLAASSHIYV
                     IDGGSSDDTVTTAEAFGATVIIQRGKGYGGAIRTAFEDIQATWLLTMDADFSHHPVCI
                     KYLFSRRYDAEVVIASRHVYQGYGDMPLSRRILSGSLNRVFAKVLSMKVGDLSSGFRL
                     YHRNAIAGLELTYETYAVLQEILVQAYCEGFRVAEEPFHYLPRLHGASHARVFQFGVV
                     YMKALRALWKRRNSSDSADYETRAFYSLIPLQRWWQRRRYRLLRDLVGDRMQMLDVGC
                     GSSQILNCMPQMVGIDCRHSKLRFMRRTARNLANADAAALPFRDNFFETILLSQVLMY
                     VEDPAEVIRELFRCLKPGGELIVGVPDSGKWQWRLLGSIYGLLHPDGRKGKRFTGEEL
                     KRLLGQNGFHIEESRYILGAELILLARKSA"
     gene            complement(9191..11419)
                     /locus_tag="PKN92_07220"
     CDS             complement(9191..11419)
                     /locus_tag="PKN92_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ48620.1"
                     /translation="MNRSDVDKNTTEFAKCTELVQEKAGRGEYKYNFSDLIAASVALP
                     LHLLVSVPLLLFLNNLQEFYSTSGTMLLYLAGIAAAAAVLLFFILYFSGSRIKNFLIP
                     VLAFILLSTWVQSQLLSWHYGSFDGSPLEIHLRDGRAFDGLIWLSLAALCFGIARKKP
                     GLFKNVFLVFLFFTAGNLVFSVAQPRERIAGTGTSESITLSWRNAFSYSSRSNIILLI
                     LDAFQSDYFNEYLEKNPDYAKKLPGFTYYPDALATDYYTHRSLPAIMSGQHYNNEGSY
                     TAYLQNAYKTFSVPAKLHDAGYSVGIYNYFTLAQSMYRPEFLKSFADNYKSDGEDFTP
                     YYGKEVRQLLWTSLFQAVPHLCKDAVYSNFIMDVPANLDRDIFRKDLKSQRRVAFSEP
                     RFLVYHLQGLHDPQYLDGKKVPDTRENVSRIAEILAELIEDMTGSLQRLGIYDRTALM
                     IVGDHGLQWTNSNIHYGNYSAGNKPAEPPLGSFAKKVRAIPLVLFKPMGQSGGFQVSD
                     RPVSLLDIAPTLLESAGLETAGSYGGESLLQPGSRPRIRHFYTSEYTNRGKGALYEYA
                     VSGFSWYDSSWTFTGNSYTGKGVKRVLLDNCLPWKLYSCGMAGTGRQFFDEGWAPDGE
                     REHVLVESVGTITLPLADPAKNYRLQLYFSNTQTETEVIVRIAGQEQGKYSLRPDCSI
                     DLEVPGSLLTTVPNQPEASPVPAKAHLAPVPVPSAYRMSVSLCKTEAVSVKLSSLELT
                     AL"
     gene            complement(11635..12936)
                     /locus_tag="PKN92_07225"
     CDS             complement(11635..12936)
                     /locus_tag="PKN92_07225"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNZ48621.1"
                     /translation="MEKLVSLCKRKGFIFQSSGIYGGINGCWDFGPLGVELKRNLKEH
                     WWYTFVLSRDDVVGLDASILMHPGVWEASGHVEQFHDILVDCKECKKRFRQDHLEQDT
                     CPECGGELSEPRAFNSMLRTKMGVSEDTGVETYLRPETCQSIFVDFKDVCSSTRVKVP
                     FGIAQVGKSFRNEVNPRNFIFRSREFEQMEIEFFCKEEEEELWFDRWQKDIWAWYLNL
                     GLKEDKLRWFEHPKETLAHYSKRTIDVEYDFPFGWGELQGLANRGCYDLTQHSKHSGK
                     DLSYFDAELKERFFPTVIEPSAGADRTTLAVLCDAYSEETVNDEMRVVMRFHPKLAPI
                     KAAVLPLVKKDGLAEIAEGLEKKWRGFFTTYYDQAGAIGRRYRRMDEVGTPYCVCIDY
                     ETKENDTVTIRERDSMEQVRMPVSEVPAYILKQCSIRPVCF"
     gene            complement(13296..14534)
                     /locus_tag="PKN92_07230"
     CDS             complement(13296..14534)
                     /locus_tag="PKN92_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48622.1"
                     /translation="MGTRRDFLKTAAGAAATLTAMGYTRSAGANDRLSIGLIGCGSRM
                     KGSIMPSINNYAKKYNVEITAVADPWRVAREEAAGICEGWFGKAPQQYVSYRQLLDEA
                     QVDLIAIASPEHLHTTHLKAVAEAKKDVYCEKPLAKELAPLRDAYDAVKANGIVCQVG
                     TQLRSYPAMLGAREYYKTGALGKVGRIEQHRNSARPYWYSYLKEDVREEDVDWAEFLG
                     HCPMRPFDPILFSGWYGFREFSDGAVPSWGAHYIDLVHSITGAGIPKACVAMHTTQIW
                     KDDYGFDCPDHANVVWEYEDGFSAHYSTYFGNGSGNNFLIMGNQGVMDLQDWSNVYVS
                     SQGAFGDSPLSKDRVRIDDVNGPDHIEDWLQCVRERKTPNANIEAGYQHAIAVIMAMR
                     AADTGRRQIYNRASREILDA"
     gene            complement(14687..16108)
                     /locus_tag="PKN92_07235"
     CDS             complement(14687..16108)
                     /locus_tag="PKN92_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ48623.1"
                     /translation="MKKLRIFLVNVGLRSRLYPLATPPLGIMYIAAYLRERFPLEIKL
                     VNQRVEDCSHDDIIRQIIDFDADIVGLGSMTPTAHGMPVIARGVRDAKPDTFIVLGGP
                     HVSAFGKPALEHCPAADAAVAGEGELSMEMILRALHEGGGFSHIPGLMWRDKEGEIIT
                     NPGRAPVIEDLDTIPMAAYDLIDLSKYWRTQSMPPIPRRRYVSLLSSRGCPYACMWCH
                     NIFGRAFRAHSAARIVDEVRHYVKTYGIDDIEFVDDSFNLSKERLLDFSDLLLKTNGP
                     IKIAFPNALRADLLEESTVDALVDAGVYFCSFALESGSPRIQEFTGKRLNIPQFLKAI
                     ELSTRKGIFANGFMMLGFPTETEEEMQETIRVATDSQLHTASFFTVTPFPGTALYEYV
                     KENLQERLKGMDYNDMDFCTMHVNMSTVTDERLFYYQRKAARTFFFKPSRIYRILRDF
                     PQPHLLPLYVPIFLNRAIKGIFS"
     gene            complement(16254..17672)
                     /locus_tag="PKN92_07240"
     CDS             complement(16254..17672)
                     /locus_tag="PKN92_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ48624.1"
                     /translation="MKKLRIFLVNVGLRSRLYPLATPPLGIMYIAAYLRERFPLEIKL
                     VNQRVEDCSHDDIIRQIIDFDADIVGLGSMTPTAHGMPVIARGVRDAKPDTFIVLGGP
                     HVSAFGKPALEHCPAADAAVAGEGELSMEMILRALHEGGGFSHIPGLMWRDKEGEIIT
                     NPGRAPVIEDLDTIPMAAYDLIDLSKYWRTQSMPPIPRRRYVSLLSSRGCPYACMWCH
                     NIFGRAFRAHSAARIVDEVRHYVKTYGIDDIEFVDDSFNLSKERLLDFSDLLLKTNGP
                     IKIAFPNALRADLLEESTVDALVDAGVYFCSFALESGSPRIQEFTGKRLNIPQFLKAI
                     ELSTRKGIFANGFMMLGFPTETEEEMQETIRVATDSQLHTASFFTVTPFPGTALYEYV
                     KENLQERLKGMDYNDMDFCTMHVNMSTLPDDVFFSYQRYAFQQFYLKPSRLFRLMQVY
                     PKPYFLPFYLPLFMSRLFKELF"
     gene            complement(17818..>18345)
                     /locus_tag="PKN92_07245"
     CDS             complement(17818..>18345)
                     /locus_tag="PKN92_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48625.1"
                     /translation="MKQRNSHNDWTKEWGLDTRIQKFMGKNLNIPAFLKTAEWFDKKK
                     VFTNGFVMLGFPTETEAEIKTTIKVATDSALHVASFFTVTPFANTALARYVEEHMPDK
                     SFTPEMNDMEYWSAKINLTGLPDDVFFSYQRYAFQQFYLKPSRLFRLMQVYPKPYFLP
                     FYLPLFMSRLFKELF"
BASE COUNT         4617 a         4804 c         4418 g         4506 t
ORIGIN      
        1 ggggaaatca tgaccatctg gacgattacg acaaaggaca taaagccctg gcgcaggcac
       61 gccattccct gtatggcggc acaagcaaaa acgggggaca ttatgtgacc cggataaaag
      121 accgaagcga caggatactg tttgagcttt tctgcctcaa tacagaggaa cgcggtgttg
      181 gcgaaggcat caagaatttt ctcaggcaga tgcttagcca ggaacaaagc gatcaaaaac
      241 gtcctatgcg tctcggtgct tttcatatcc ccctgcgtca gtatcgggat gtatgggaaa
      301 acggcaatgc attcggcgta aaaggagaag agatttgctt cgaagaggaa gacggctccg
      361 cattgccgct tttacgggaa gccggaattc aggctgtcat ttgcggacat gatcatacaa
      421 acgattattc cggttttgcc gaagggattg aactgattta tggccgtgcc tccggctaca
      481 acggctacgg aaatcaaacg ctcaaaaaag gcgcaaagct gtacagaatc gacccggcaa
      541 ctgccgcatt gcactgggaa tcatatcttc ccgatggaac ccgctggcat cccggtccaa
      601 cggaacgccg cagcatcgcc ccgtaaatct tttcctgcag gaaaccccgg ccgatactct
      661 tatctgctct ctgtctttca gtgccaagtc gcaagcgggt ggtttcgctc ctgaagcgga
      721 aacgccaccc gtctgcctgc gacgtgtgat gcaaatacag cctgaatcat ctccgtcgca
      781 gccagagcat cctgtatgct caccgccgga tgtctatcct gcactatggc atccatcagg
      841 tcatcaatga tcggcgcata ctggctgacc gctcccggcg accgcagcgg cgcaacaggc
      901 atatccggca aagcctgcca gacttcttcg gttccgcacg atgaccatcc gggctcaggc
      961 aggtagctga tgatcggatc acgatccacc cggatggatg cggctccttt ggtgccgtaa
     1021 atatccatgc catatcggtc ggcatcattt gatgcccctt tgaccgacgt gaaataaccg
     1081 cgcctgccgt ttttaaaacc gaactgggca aaaatggtat ctccgacaat cggacccagc
     1141 ccttctgtcg cttcatgaac atcagcggga agagccgggc ggccttctgt cagaatctcc
     1201 gcctcacacc acaggggctt cccgaggaaa taaatcatga ggtcaaagag atgaataccc
     1261 agcacaatca gatcttctcc gccggaacga tgatcttctt tgccgcgggc acgcacttca
     1321 agaacgctgc caatcagccg ctcattgatg attgatcttt ttgcatgaaa aatccctgga
     1381 gctgcacgaa aattaaaacc ggctgcccac tttaaattct tcgcttccag tgccgcaacg
     1441 gcagcatcgc cgcccgtgag gtcaggagtc agcggctttt ccataatgcc gtgtgcaccg
     1501 atagcagcgc acgcttccag atattcctga tgacaggttg tccagcgggg ggcaacagaa
     1561 accagatccg gtttctcttt ttccagcatc tcccggtagt ccgtataaat ccgcttcgcc
     1621 ccggcttctt ccccgtgctt gcgtgccccc tcttcatcgg gatctgccag cgcaacaagc
     1681 tccacatcat tccgcagtcc ccagatcaga tgaaagccgt gtccgtagcg gccccgctgg
     1741 ctgtcaccga tcacgcacgc ccgataggtc tgttttttct cctgcgcagc ccatgttgca
     1801 aaagggctta gcgatgctgc agcagaagca taaagaaaag aacgtctgtt gatgaaaaac
     1861 atagggagac cctttcttgt cttcagaata gcacagcccc tttccgtttt tcattaacaa
     1921 taaaaaagtg ctctctatcc ggagggacaa agagcactcg gatttaagct cttacggctg
     1981 ctcagctgtc agccttttcc tgcggcacgg cttgaaaaga aagctcgcct gaatcgaccc
     2041 ggacaagaat atgatcgcct tctttaatgt cgccggcgat aattttatag gcaacaggat
     2101 caagcagata ctgctggata gcgcgcttca gcgggcgtgc gccataagcg ggatcgaaac
     2161 cgcgctccgc cagaagctct tttgccgcat catccagatc aagtgtaagc ctgcgacttt
     2221 ccagacgctc ggcaagacgc ttcagctgaa cgtccacaat ggcacggata ttgtccctgc
     2281 tcaaaggatg aaacaccact gtttcatcga ttcgattgag aaattcagga cggaaatgag
     2341 cccgcaggag agcacgaacc gcgtcactct tctcagcatc ggtgttgtcg ctctcattaa
     2401 agactgtgct accgatgttg gaagtcataa tcacgaccgt attccggaag tccacagtac
     2461 ggccctgatt atccgtcagt ctgccgtcat cgagcagttg caggaggata ttgaaaacat
     2521 cgggatgcgc tttttcaatt tcatccagaa gaatcacact gtacggcctt cttcgaacgg
     2581 cttccgttaa ttgtccgcct tcctcataac ccacataccc gggtggcgct ccgatcaggc
     2641 gggacacggc atgtttctcc atatactcgg acatgtcaat gcgaaccatg gcattttcat
     2701 catcaaacat gctttcagcc aaagcccggg caagctctgt ctttccaacg cccgtaggcc
     2761 cgagaaagat aaaggaaccg ataggacgat cgggctcttt cagtccggca cgggcacgac
     2821 gcaccgcttc ggaaactgcc ttgatgccgt cactctgtcc gattacacgc tgtccgatct
     2881 ctttttccag agaaatcagc ttctgcattt cgctttccat aagccgatca accggaatcc
     2941 ccgtccagga tgaaacaatt ttggatacat gttcttctgt tacttcttca ctcaaaaaac
     3001 tgccattttt ctgtatgtca gccaggcggg cattctgtcc ggcaagttgt ttctgcagct
     3061 cattgagcgt gccgtagcgg atttcagcca ctttatccag ctgaccctgc cgctcggcaa
     3121 tttcctcctg acgtcgcgcc tcatcaattt tgctctgtat ggaacggatt gtttcaatac
     3181 agtctttttc tgttttccat tgcgccgtct tcgccgacaa gtcttctttc aggtttgaca
     3241 tttctttctc gagtgcagcc agtcgctcct gagcgctttt atccttttct tttttcaggg
     3301 cgagatgctc gatttccagc tggcgaagac gtcgggcaat gacatctatt tcgtagggca
     3361 tgctgtcaat ttcaatacgc agacggcttg cggcctcatc gatcaggtca atcgctttat
     3421 cagggagaaa gcggtcagca atatagcgat ggcttaaggc tgccgcggcg accagagcat
     3481 cgtcctgaat gcgcacacca tggtgcactt cataacgttc tttgagcccg cgcagaatcg
     3541 ctatggtgtt ttccacattc ggctgatcca caaacacggg ctgaaaccgg cgctccaagg
     3601 cggcatcctt ttcaatatat ttgcggtatt cgtcgagtgt cgttgcgccg atgcagtgca
     3661 gctcgccccg tgccagcgca ggcttaagca tgttcgatgc atccatggag ccttctgaag
     3721 cacctgcccc cacaatggta tgcatttcgt caatgaacag aataatctgt ccttcagctt
     3781 cctgcacagc ggacagcacc gccttcaggc gctcttcaaa ttcaccgcgg aactttgcac
     3841 cggccaccaa tgcggcaata tccagcgaag caacctgttt gtttttcagc ccttccggta
     3901 tgtcgcccgc aatgatccgc tgtgccaatc cttcaacaat cgctgtctta cccgtgcccg
     3961 gctcaccaat cagcaccgga ttatttttag tgcgtcgtga cagaacctga attacccgcc
     4021 gtatctcttc atctcgcccg atgaccggat ccaatttccc ggcacgcgct aactgggtca
     4081 ggtcgcggct gaatttctgc agcgcctcgt aggttgcttc ggcgttggga tcagtcaccc
     4141 gttgtgtgcc acgcacatct ttcaatgcct gcagcacaga agcggtatcc attcccgccc
     4201 ttttcagcag ggctgcggcc tctccgccct tttccagaat acccagcaga atgtgctctg
     4261 tgcttaaata ttcgtctttc agatattgcg cctgtttcca ggcagcatca aaaactgccc
     4321 tggcatcccg ggaaaggccc ggctgtcctc cgccgctaat ccgcggaagc gaatctaaag
     4381 cacggtcggt gtcagtcaaa atctgtgcag gggcaacacc gatgcgctgc aggatagacc
     4441 ccacagcgcc ctgctcctgc ccggcaaggg tgcgaaggaa gtgcaaaggc gtaatttcag
     4501 ggttgccccg gtttgaggca agctccaaag cttccgcaag tgcttcctga gattttattg
     4561 tcaatttatc catacgaatc atttttctct tcctttctga tctacaagta gtatactgat
     4621 tactactgag caaagagcat gcctaaactt ttttagcatt tcccttctat tgcaaaggaa
     4681 acatgatcca tagtgaaaca gtctgtacag gtatgaaaca cacttcccgg ttatgaagca
     4741 tatatgtcac ctgaaataca caggatctcg aataaaatga gtatgaagag aaaactgagc
     4801 attttcgctg ccatagtggc gggaaccctg tttttggcgg cttcttgtct tttttatctg
     4861 cttttccacc gctcgccggg tcagtttttt gaacaggacg gcactcgcct tttctattcc
     4921 gatacaggcc gtggcgaagc tgttattctg gtgcacggca tagcagcaca gggggacctt
     4981 aactggcgcc gccctggcgt gacacgcatg cttgcaaagc agttccgggt tattactctg
     5041 gatatgcgcg gtcacgggct gagtgatcgc ccggaagatg cggaacacta cggcattcac
     5101 ctcgtaaccg atatcattgg cctaatggac cacctctcca tcgacaaggc tcaccttgcc
     5161 ggctattctc ttggcggatt tgtcgctctt aaagcagtta cgcaatatcc tgaccgtttc
     5221 ctcagcgcag ccatctgttc ggcgggctgg attaatcctg aatcacccgg cgagattccc
     5281 aatccttatc aagcaccggt taaaaacgct gaaatcaaaa ccagccatgc ttctgtaatg
     5341 atgccggtcg cttctaaatc ggtttttcat cggatccgaa gcggcgtggg cgatatgttg
     5401 attgacagaa aaatcaagaa ggcacttaaa gcaagctatc cggagctggg cgtcctccgg
     5461 gaggacgtgg aaaccatcaa aacgccactg ctctgcatca tcggcactaa agacggtttc
     5521 ctgcaccttg cacgcgctct ttgccagttc gagtgccctc tggaatgtat tgaaattggc
     5581 ggcgcaactc atttcagtgc gcctttcaaa agcgaattta aaaagaatct gcttctgttt
     5641 tttcagaaaa acagcccccg gcacaaataa ggaaagggcg gatcctcagg gaatccgccc
     5701 ttaaaacggg tcaggtaccg ataaggcgtc ttttggaagt cagatcagac aagtcactgt
     5761 ggaatcagga gccttctttt tcagaagagc cgctgtcaga agaagaactg tcggactttt
     5821 ctttttcttt gttgcatttc tcgctgcagt cttttttatc agcggctttt tctttggagc
     5881 actcgccact taccgggcag gcaggcgctg ccgccgctgc aacgggacag gacgcgctct
     5941 ttgcacaggc tgacttgcaa cagctgccag ctttggcgga atttccatct tctgctactg
     6001 ccatcataga aaaggcagca accatcatca caacagccaa gacagagacc atgcttacta
     6061 cacgcatatt ctttttcatg ttttctttct ccttggttta cgctaacaac cttttgttta
     6121 gcatgctgat catacaaaca acacaaactt ggtttttatt cgcaaaaact gtttatttgt
     6181 aaaatgtttt ttccgtcctc gtataacagt gaaactgaga ggattgaaac ccctgtctaa
     6241 tgacaggatc atatgttttc atgttttttg attcaacggg cacagggtac tataattcag
     6301 taagcaacta ttgaggagaa tcaacatgcc ccctatcaca cgccgacagt tcctgcaatc
     6361 agccacgctc cttgccggca gcgctctgct gacgcatcct ttgaaagtct ctgcccgtgt
     6421 tatcggcgcc aatgaccgcc tgcgtatcgc cgttgcgggg ctcaatggcc ggggtcaggc
     6481 gcatatcagc ggctggcttg agcagaataa cgttgaaatc gcctatctga ttgacccgga
     6541 agcgcgggtg cttgcccgtg ctctgaaagc ttttgagaaa aaagcaggcg gtcaccctgt
     6601 tcccaaaggg gtcgccgata tcagagaagc tttgaacgat cctgaactgg atgctatatc
     6661 cattgccaca cccaaccact ggcattctct gatgaccatt tggggcgcac aggcaggcaa
     6721 acatgtgtat gtggaaaaac cgatgagcca tgatatcgga gaaggccgtg tggttgtcgc
     6781 cgcccagaag aaatacgggg tggtcattca gcatggtact caggcgcgca gcaatctgca
     6841 tacggcagga cttcacgagg ctattcatgc aggaaaattc ggtcgtttaa aaattgccta
     6901 cggttactgc tgtaaacccc gggcaagcat caaatttgca gaacctgccg aaccgccgga
     6961 atatctggac tggaatcttt ggtgcggccc ggcgaaagtg gaacactacc accgcaatta
     7021 tgtgccttat aactggcact ggttctggaa tacgggcaac ggcgacctga acaatcaggg
     7081 cacccatcag ctggatgtcg cgcgatgggc aattgatccc gatcagaccc atccggtacg
     7141 tgccatggca ataggcggcc gctttcagtg gaatgatcag ggcgagacac ccaataccat
     7201 gttcagcctg gctgagtatc ccaatggtca gatggtattt ttcaatgtcc gcaatgtgaa
     7261 ttatagcggc tatgaaaatc aggtggaaaa tgaatattat tttgaagacg gcggcaaaat
     7321 catccgggat aaatactacc caaaagggag cagccaggga gaaccgatcg acgttccgga
     7381 cggaaaagtg acgcccggag gcaattgggc aagttttatt acggcctgtc gtgccggtga
     7441 tccgtctctg gcaaacggca atgcgcagga atcccattac agctgtgttc tggggcacct
     7501 gatcaacaat tcctatcgtc ttggtgtaaa agcacccttc aatgtaaaat caggaaattt
     7561 cggtgataat aaagaagcct atgagcactt tgcgcggctc catgaaatta tgagcaaagg
     7621 ggtcggtatc cccgaaaacg gtaatgagta taccgtcggg ccctggctga ccttcgatcc
     7681 tgaaacagaa acccatacag gcgctttcag cacggaggca acggcattgt taaaagatgc
     7741 caaccgcgcg ggatttgaaa ttcctgccgc cgccgaagtg taagcacatc atcgggtgaa
     7801 cactttttga cagtacatcg cactgtaatg taagcgtatg cgttttatac gccttgaaat
     7861 ccttttaaca actttgattc atagtaaaga cactatatga gcagctcttc cacgctggat
     7921 cttgccgttg ttcttccatg cctgaacgaa ggagcgaatc tgcgtctcct tctcccccgc
     7981 attcagacag tccttgccga tcttgcggca tcaagccaca tctatgttat tgacggaggc
     8041 agcagcgacg ataccgtcac gactgccgag gctttcggcg ccacagtcat cattcagcgc
     8101 ggaaaaggat atggcggcgc tatccgcacc gcttttgagg atattcaggc gacctggcta
     8161 ttgacgatgg atgccgattt ttctcaccac ccggtttgca taaaatatct tttttcacgg
     8221 cggtacgatg cggaagtggt tattgcgtcc cgtcatgtgt atcagggcta cggcgatatg
     8281 cctttaagca ggcgtattct gagcggatca ctcaaccggg tttttgcaaa agtactcagc
     8341 atgaaagttg gggatctctc cagcggtttc cgcctctatc accgcaatgc cattgccggg
     8401 ctcgaactca cctatgagac ctatgccgtc cttcaggaga tactggtgca ggcatactgt
     8461 gaaggattcc gtgttgccga agagcccttt cactatctgc cgaggcttca cggagccagc
     8521 catgcccggg tgtttcaatt cggcgttgtc tatatgaaag cccttcgtgc actctggaaa
     8581 cgaagaaatt cgtcagacag cgccgactat gaaacccgtg ctttttacag cctcatcccc
     8641 ttgcagcgat ggtggcagcg acgcagatac aggctcctgc gtgatctcgt tggcgaccgc
     8701 atgcagatgc ttgatgtggg ctgcggatcg agccagatac tgaactgcat gcctcagatg
     8761 gtgggcattg actgtcgcca cagcaagctg aggtttatgc gcaggactgc cagaaatctc
     8821 gccaatgccg acgccgccgc tcttcccttt cgggacaact tttttgaaac cattttgctg
     8881 tcgcaggtac tcatgtatgt tgaggacccc gctgaagtga ttagggagct gttccgatgc
     8941 ctgaagcctg gcggagagct gatcgtgggg gtgcctgact ccgggaaatg gcaatggcgg
     9001 ctgctgggaa gcatttacgg acttttgcat ccggacggca gaaaagggaa acggttcacc
     9061 ggtgaagaac tgaagcgact gctggggcaa aatggctttc acattgaaga aagccggtat
     9121 attctgggcg cagaactgat tttgctggcg cgtaaaagcg cgtagagcgg tctctaatca
     9181 tccggatgta tcagagcgct gtcagttcca gggaagacag tttgacagat actgcttccg
     9241 tcttgcacaa cgacactgac atccggtacg cagagggcac ggggaccggg gcaaggtgcg
     9301 ctttagcagg aacaggtgac gcctccggct gattcgggac agtggtaagc agtgagccgg
     9361 gcacttccag atcaatactg caatccggtc tcagagaata cttgccctgt tcctgtcccg
     9421 caattctcac aatcacttct gtttccgtct gtgtattcga aaaatacagt tgcagccggt
     9481 aattctttgc cggatcggcc aggggcagtg taattgtgcc cacagactcg acaagaacgt
     9541 gctccctttc accatcaggc gcccagcctt catcgaaaaa ttgccggcct gtacctgcca
     9601 tgccgcagct gtacagtttc cacggaagac aattatcaag gagtactctt tttactcctt
     9661 tgcctgtata gctgttgcct gtaaaagtcc atgacgaatc ataccaggaa aatcccgata
     9721 cggcatattc gtacagagcc cctttacccc ggttcgtgta ttccgacgtg taaaaatggc
     9781 ggatgcgtgg cctgctgccg ggctgtaaaa gcgattctcc gccgtagctg cctgcagtct
     9841 cgagacctgc actttccagc aaggtaggcg caatgtcaag aagagataca gggcggtctg
     9901 acacttgaaa gcctccgctt tgccccatcg gcttaaataa aacaagggga atagcacgca
     9961 cctttttagc aaagctgcca aggggcggct ccgccggctt attaccggca ctgtagttac
    10021 cataatgaat attgctgttt gtccactgca gaccgtggtc gcccacaatc atcagggctg
    10081 ttctgtcgta aatgcccaat cgctgcagcg acccggtcat atcttcgatt agctctgcca
    10141 gtatttcagc aatacgactc acattttcac gtgtatccgg cactttcttt ccgtcgagat
    10201 actgaggatc atgaagcccc tgcaggtgat agacaagaaa acgcggctcc gaaaaggcaa
    10261 cacgtctctg gcttttcagg tctttacgaa atatatctct atcgagattg gcgggaacat
    10321 ccataataaa gtttgagtaa acagcgtctt tgcagaggtg cggcacagcc tgaaaaagag
    10381 atgtccaaag cagctgccgt acctctttgc cgtaataggg cgtgaagtct tctccgtcgc
    10441 ttttgtaatt gtcggcaaac gatttcagaa actccgggcg gtacatactt tgtgccagcg
    10501 taaaataatt gtaaatgccc accgagtacc cggcatcatg cagcttcgcc ggaactgaaa
    10561 aggttttata agcgttttgc aggtaggctg tataggagcc ttcattattg tagtgctgtc
    10621 cgctcatgat tgcaggaaga gaacgatgcg tataataatc tgtagcgagc gcatccggat
    10681 aatacgtaaa cccgggcagt tttttcgcgt agtccggatt cttttccaga tactcattaa
    10741 agtaatcgga ctggaaagcg tcaaggatca gcagaataat gtttgaccgg ctggaataac
    10801 tgaaggcatt tcgccagctg agtgttatgc tttcagaagt acctgtcccg gcaatacgtt
    10861 cgcggggctg tgctacggaa aaaaccagat ttcctgcggt gaaaaacaaa aaaacgagaa
    10921 aaacgttttt aaaaagccct ggcttttttc gggcgattcc gaagcacaac gccgccaggc
    10981 tcagccagat cagcccgtca aaagcgcgcc cgtcacgcag atgaatctcc aggggagaac
    11041 cgtcaaaaga gccgtaatgc cagctgagca gctggctctg cacccaggtg ctgagcaaaa
    11101 tgaaggcaag cacaggaatc agaaagttct ttatgcggga gccggaaaaa tacagaataa
    11161 aaaagagcag cacggctgcc gccgctgcta tgcccgccaa atagagaagc atggtacctg
    11221 atgtgctata aaattcctgc agattattga ggaataaaag cagcggcaca gaaacaagaa
    11281 gatgcagcgg caaggcaaca gatgctgcga tcaaatctga aaaattgtat ttatattcgc
    11341 ctcgcccggc cttttcctgc accagttcag tgcattttgc aaattcggtt gtatttttat
    11401 ccacatcgct tctgttcata aacacaccaa attgacttgc cggccggaat atgatagtat
    11461 gtagatctgt ttagtcaaga taaagacagg tcgtttggaa aagtcatagc acaaaccttg
    11521 tgcaccgttc gctgccaccc acaaacacgc atgccccacc cttttggcgg acggtgcact
    11581 ctttttttat ctgccgtttc agggaagccc ggcttgcgat aaaaaagctg ctctttaaaa
    11641 acagaccggc cggatggaac actgctttaa aatatatgcc gggacttcag atacaggcat
    11701 gcgcacctgc tccatactgt cccgctccct gatcgtaact gtgtcattct ctttggtctc
    11761 ataatcaatg cagacacagt aaggcgtgcc cacttcatcc atgcggcgat accgccgtcc
    11821 gattgcaccg gcctgatcat aataagtggt aaagaatccg cgccacttct tctccaggcc
    11881 ttcggcaatt tccgccagac cgtctttttt taccaaagga agaacagctg ctttaatagg
    11941 cgccagtttc ggatgaaagc gcattaccac gcgcatctcg tcattcactg tttcttcaga
    12001 ataggcatca cagagcaccg ccaaagttgt acgatccgcc ccggcagagg gttcaatcac
    12061 tgtcggaaaa aagcgttctt tcagttctgc atcaaagtaa ctgaggtctt ttccggagtg
    12121 cttgctgtgc tgggtcaagt cgtaacagcc tctgtttgca agcccctgca gttctcccca
    12181 tccgaaggga aaatcatatt caacatcgat agtccgttta gaataatgtg ccaaagtttc
    12241 tttgggatgt tcaaaccatc gcagtttatc ttccttaagc ccgaggttca ggtaccacgc
    12301 ccagatatcc ttctgccagc gatcaaacca gagttcttcc tcttcttctt tgcagaagaa
    12361 ctcaatctcc atctgttcaa attcgcggct ccggaaaata aaattccggg gattcacttc
    12421 attgcggaaa ctttttccca cttgtgcaat gccgaagggg accttgacac gtgtggaaga
    12481 acagacatct ttaaagtcca cgaaaattga ctggcaggtt tcgggacgca gataagtttc
    12541 cacgccagta tcctcgctca cccccatttt agtccggagc atgctgttga atgcccgtgg
    12601 ctcggacagt tcgcctccgc actcggggca ggtatcctgc tccagatgat cctggcgaaa
    12661 gcgtttttta cactctttgc agtccacaag aatatcatga aactgttcaa catgcccgct
    12721 tgcctcccag acaccgggat gcataaggat gctggcatca agacccacca catcatcccg
    12781 cgaaagcaca aaggtatacc accagtgctc tttcagatta cgcttgagtt ccacgccgag
    12841 gggaccgaaa tcccagcagc cgttgatgcc accgtaaatg ccgctggatt ggaaaataaa
    12901 gccttttcgt ttgcacagac taaccagttt ttccacgtta tgctcctgtc ctgatgaaga
    12961 taaatcgttt aagcgcttca gtccgcgaaa ggtgtgggcg ggataagccc gatttctgta
    13021 tgccgggctt aagttcagta caggatagta taaagagggc tcttgagttt tgtcaaacct
    13081 tattttggag acatccgata ttttttagta acaaccgtcc ttaaatcggt ttttacgccg
    13141 ccttccctgt tttatctgat tctgtacgcc tctttccaaa aaaaaggccg gatgcccagt
    13201 ttactgctcc cctctcatcg gggaaacaaa gactcaggac atccggccga cactcagtat
    13261 aaactgttca aaagaaaagt caggcgacca actaatcagg catcgagaat ctcgcgactg
    13321 gcgcggttat agatctggcg acgccccgta tccgcagcgc gcatcgccat aatgacggca
    13381 atagcgtgct ggtatccggc ttcaatattt gcattgggcg tttttctttc acgaacgcac
    13441 tgcagccagt cttcgatatg gtcgggccca tttacgtcat cgatgcgtac ccgatctttg
    13501 gacaaaggcg agtccccgaa tgcgccttgc gaagaaacat agacgttgct ccagtcctgc
    13561 agatccatca cgccctgatt gcccatgatc aggaaattat taccggaacc gttgccgaaa
    13621 taggttgagt agtgcgccga gaagccatct tcatattccc acacaacgtt tgcatgatca
    13681 gggcagtcaa agccgtagtc gtccttccat atctgggttg tgtgcatggc aacgcaggct
    13741 ttcgggatac cggcacctgt gatggaatgt accaggtcaa tataatgggc accccagctg
    13801 ggcaccgctc catcggaaaa ttctctgaag ccgtaccagc cgctgaagag aataggatca
    13861 aagggacgca tgggacaatg acccagaaac tccgcccagt ccacatcctc ttcccgtaca
    13921 tcttccttca gatagctgta ccagtaggga cgggctgaat tgcgatgctg ttcaatcctg
    13981 cccacttttc ccagagcgcc tgttttgtaa tattcgcggg cgccgagcat ggcgggataa
    14041 ctgcggagct gcgtacccac ctggcacaca atgccattgg cttttacagc atcgtaagca
    14101 tctctgagag gggcgagttc cttcgccagc ggcttctcgc agtagacgtc ctttttggct
    14161 tctgccaccg ctttcagatg ggtcgtatga agatgttcgg gactggcaat ggcaatcagg
    14221 tcaacctgtg cttcatcaag cagctgcctg taactgacat attgttgcgg ggcttttccg
    14281 aaccagcctt cgcatatgcc cgccgcttct tcccgtgcca ctctccaggg atctgctaca
    14341 gccgtaattt ccacattata tttcttggcg taattgttga tggagggcat aatggagccc
    14401 ttcattctgc tgccacagcc aatcagacct atactaagtc ggtcattggc acctgctgaa
    14461 cgggtatagc ccatagcagt caaagtggcg gctgcacctg ccgctgtttt taaaaagtca
    14521 cgtcgtgtac ccatgtgtga atcctctctt tttcagttga acataagatg tgcttaaaaa
    14581 agacaggtca attatgcgct tcatggcagg cgaatgcaaa cctgccctgc agcatacggc
    14641 atgcaaaacc tgtcgcgccg tatgcaggtg gcggaacgat gcatgattac gaaaaaatcc
    14701 ctttgatcgc tctgtttaaa aagatgggca catataaagg aagtaaatgc ggctgcggaa
    14761 aatcacgaag gatacggtaa atcctgctcg gcttaaagaa gaaagtgcgc gctgccttgc
    14821 gctggtaata gaaaagcctc tcgtcagtca ctgtggacat gttcacatgc atggtgcaga
    14881 aatccatgtc attgtaatcc atgcctttca ggcgctcctg gaggttttct ttcacatatt
    14941 cgtacaaagc agtaccgggg aaaggagtca ccgtaaagaa agaagccgtg tgcagctggg
    15001 agtctgttgc gacccgaatc gtttcctgca tttcctcttc tgtttctgtc gggaacccca
    15061 gcatcataaa tccgttggca aaaatgcctt ttcgcgtgga cagctcgatc gcttttaaaa
    15121 actgaggaat gttgagccgt ttccccgtga attcctgaat acggggggag ccggactcca
    15181 gcgcaaaact gcagaaataa accccggcat caaccaaggc atcaacggtg ctttcttcca
    15241 gcagatcagc gcgaagtgca ttgggaaaag caattttgat cgggccgttg gttttaagca
    15301 gcaaatcgga aaaatcgaga agacgctcct tgctcaggtt gaaagagtca tcaacaaatt
    15361 caatatcatc gatcccatag gttttaacat agtggcgcac ttcatccaca atacgtgctg
    15421 cagaatgagc acggaatgca cgtccgaaaa tattatggca ccacatgcag gcataagggc
    15481 agccccggct gcttaaaagg gacacgtaac gccgccgtgg aatggggggc atggactgtg
    15541 tccgccagta tttagaaaga tcaatcagat cataagccgc catcggtatt gtgtccagat
    15601 cttcaatgac gggtgccctg ccgggatttg taataatttc gccctctttg tcacgccaca
    15661 taagcccggg gatatgacta aatccgccgc cttcatgcaa cgcccgaagg atcatctcca
    15721 tgctcagttc accttctcct gcaacagccg catcggcggc aggacaatgc tccagagcag
    15781 gttttccaaa ggcagaaaca tgagggccgc ccagcacgat aaaagtgtcc ggcttggcat
    15841 cccgtacccc gcgggcaata acaggcatgc catgggcggt tggtgtcatg cttcccagac
    15901 ctacgatgtc cgcatcaaag tcaatgatct ggcgaataat atcatcgtgc gaacaatcct
    15961 ccaccctctg gttaaccagt ttaatttcca gaggaaaccg ttcgcggagg taggcagcga
    16021 tgtacataat tcccaaaggc ggtgttgcca agggatacaa acgtgagcgc aatcctacgt
    16081 tcaccagaaa aatacgtagt tttttcataa attatctcct gcacacatgc aggtttcctt
    16141 ttatgccggg acttaaacaa gagtttaagc tgcacacaga gattattcac cgaaatgtat
    16201 accaatgcat aacatgtacg cagaaaaaat taagtttatc acatttgtgc tatttagaaa
    16261 agttctttaa aaagtctgct cataaacaaa ggcaggtaaa atggaagaaa gtacggtttc
    16321 gggtaaactt gcatcaggcg gaataatctg gacggcttga ggtaaaactg ttgaaacgca
    16381 tatctttgat aagagaaaaa tacatcatcc ggaagagtgg acatgttcac atgcatggtg
    16441 cagaaatcca tgtcattgta atccatgcct ttcaggcgct cctggaggtt ttctttcaca
    16501 tattcgtaca aagcagtacc ggggaaagga gtcaccgtaa agaaagaagc cgtgtgcagc
    16561 tgggagtctg ttgcgacccg aatcgtttcc tgcatttcct cttctgtttc tgtcgggaac
    16621 cccagcatca taaatccgtt ggcaaaaatg ccttttcgcg tggacagctc gatcgctttt
    16681 aaaaactgag gaatgttgag ccgtttcccc gtgaattcct gaatacgggg ggagccggac
    16741 tccagcgcaa aactgcagaa ataaaccccg gcatcaacca aggcatcaac ggtgctttct
    16801 tccagcagat cagcgcgaag tgcattggga aaagcaattt tgatcgggcc gttggtttta
    16861 agcagcaaat cggaaaaatc gagaagacgc tccttgctca ggttgaaaga gtcatcaaca
    16921 aattcaatat catcgatccc ataggtttta acatagtggc gcacttcatc cacaatacgt
    16981 gctgcagaat gagcacggaa tgcacgtccg aaaatattat ggcaccacat gcaggcataa
    17041 gggcagcccc ggctgcttaa aagggacacg taacgccgcc gtggaatggg gggcatggac
    17101 tgtgtccgcc agtatttaga aagatcaatc agatcataag ccgccatcgg tattgtgtcc
    17161 agatcttcaa tgacgggtgc cctgccggga tttgtaataa tttcgccctc tttgtcacgc
    17221 cacataagcc cggggatatg actaaatccg ccgccttcat gcaacgcccg aaggatcatc
    17281 tccatgctca gttcaccttc tcctgcaaca gccgcatcgg cggcaggaca atgctccaga
    17341 gcaggttttc caaaggcaga aacatgaggg ccgcccagca cgataaaagt gtccggcttg
    17401 gcatcccgta ccccgcgggc aataacaggc atgccatggg cggttggtgt catgcttccc
    17461 agacctacga tgtccgcatc aaagtcaatg atctggcgaa taatatcatc gtgcgaacaa
    17521 tcctccaccc tctggttaac cagtttaatt tccagaggaa accgttcgcg gaggtaggca
    17581 gcgatgtaca taattcccaa aggcggtgtt gccaagggat acaaacgtga gcgcaatcct
    17641 acgttcacca gaaaaatacg tagttttttc ataaattatc tcctgcacac atgcaggttt
    17701 ccttttatgc cgggacttaa acaagagttt aagctgcaca cagagattat tcaccgaaat
    17761 gtataccaat gcataacatg tacgcagaaa aaattaagtt tatcacattt gtgctattta
    17821 gaaaagttct ttaaaaagtc tgctcataaa caaaggcagg taaaatggaa gaaagtacgg
    17881 tttcgggtaa acttgcatca ggcggaataa tctggacggc ttgaggtaaa actgttgaaa
    17941 cgcatatctt tgataagaga aaaatacatc atccggaaga ccggtcagat taattttggc
    18001 actccaatac tccatatcat tcatttccgg tgtaaaactt ttatccggca tatgctcttc
    18061 aacatagcga gctaaggctg tgtttgcaaa aggggtcact gtaaaaaaag aggcaacgtg
    18121 taaagcggag tccgtggcca ctttaatcgt agttttaatt tccgcttctg tttcagtagg
    18181 aaagcctagc atcacaaagc cattggtaaa cacttttttc ttatcaaacc attccgctgt
    18241 tttcagaaat gccggaatat tgagattttt tcccataaac ttttgtattc gggtgtcaag
    18301 cccccattct ttggtccagt cgttatgaga gtttcgttgt ttcat
//
LOCUS       DAOPWB010000061        18209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3118_length_18209_cov_5.462047, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000061 DAOPWB010000000
VERSION     DAOPWB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 18209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18209
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3118_length_18209_cov_5.462047"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..2712)
                     /locus_tag="PKN92_07250"
     CDS             complement(109..2712)
                     /locus_tag="PKN92_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327375.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNZ48626.1"
                     /translation="MLLRKQGRVLAVILLLVASAAGATIPLDWAALPPLPNPDGVAGI
                     FAGFHNNAVIAAGGANFSEPVWESPKVFHDDIYVLIGREGTDCRWIHGGKLPRPVAYG
                     ASASTSRGVVCLGGNDADECFRDAFLMQWQEQEQSVVMQPLPPLPAPCTNSTAAVIRD
                     RVYVASGCTGLELETAQRNFWMLDLSLFGTDGFRWEELPPWPGPSRALAVVAAQHNGV
                     ADCVYVMSGRRVNGEGVTEFLRDVYEYMPGRAEPWHYRTDTPRCIMAGTGIALGQSHI
                     FVLSGADETLFYQTDLLKDAHPGFPKEAFAYHTITDTWTAAGTTPANQVTTPAVRWGQ
                     DTVRDPVLMISGEIRPRVRTPQVWAVRPVPGASRFGTLDFVMLGIYLAAMVLLGVFFS
                     FRNKNTDDFFRGGQRVPWFVAGLSIFATMLSSITFMAIPAKSYATNWIFFLFNMTVVA
                     SAPLVIYLFLPFFRKVDAVSAYHYLEKRFNRFIRQFASASFVFFQLGRMGVVLYLPAL
                     ALRTITPLTEQQSILLMGVLSIVYCTLGGLEAVVWTDAIQSAVLMGGALFSLILMLIY
                     IDGGLFGFFQTAHAHGKLVLFQADFSVSSFMTSALWVVLLGGMAQNLISYSSDMSIVQ
                     RYMSVPDTARAKKAIWTNALACIPSSLLFFGIGTALFVFYRQHPERLDPTFKTDAIFP
                     LFIARELPAGVSGLVVAGLFAAAQSTISTSINSMSTAVVEDFIKPWRLLKTESALLRL
                     GRLLTVLFGAAGVGLALLFASSGIMSLWDQFMTILGLFGAPMCGLFCLGIFTTRANAP
                     GAMIGALAGAAGLFLVQRFTAAHFLLYAVAGIVFCFITGYAASLLFSRSERSMRGLTL
                     YTLDDTLDPDA"
     gene            complement(2717..3622)
                     /locus_tag="PKN92_07255"
     CDS             complement(2717..3622)
                     /locus_tag="PKN92_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNZ48627.1"
                     /translation="MMKLTGLIAATFTPMTDQAQINLKPVETIVDRLISRGITGLYVC
                     GSTGEGPSLTAEERKAVARAYVEAAGGRIPVIVQVGHNSIEEARALAAHAAEIGADAI
                     SAVSPSYYLPESTAVLVETLGHITDAAPELPFYYYHIPKLSGLSVNMMEFLKQAPSRV
                     PTLAGIKFSAREIEVMQQCLAFGGGRYNILFGVDETLLSGLVAGAHGAVGSTYNFLSP
                     LYQKVMRHLSEGDLEAAQRCQLQAAGIIEVILRYHGLAGLKAAMSLSGIPCGPVRLPL
                     TPLRPDEHSALEKELQVLGFPKGIG"
     gene            3829..4803
                     /locus_tag="PKN92_07260"
     CDS             3829..4803
                     /locus_tag="PKN92_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNZ48628.1"
                     /translation="MKPSLLLVLVLGVSIFTGYAGAEELRVGILADASSDFWTKLRDE
                     AQTAAKSLDMTLDFRVPAPPTAEQQEALAQKMIDAGVKALAICPAKAAKQKDFLEALA
                     QRIPVIVLFKEVPNSTDLAFLGKDNRESGRLLATLIREVVPEGLKIQVLCDDQTRPAT
                     QQRIESFREALGSYAIVDAVLEDKGDRMLGWANVIDSLESRPEIAAFVGMQDYHGPMI
                     LRAVLEKNRGKWVRVVSCGSDPSTLKGLEDGTVHGLVVDDAAGSAQAIVKGLAAFARN
                     DKEGIEETKVATIPVTILKTEGTLSPEDMMNALQLQLPWVSEATPDRP"
     gene            4748..7015
                     /locus_tag="PKN92_07265"
     CDS             4748..7015
                     /locus_tag="PKN92_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48629.1"
                     /translation="MLSNYSYHGCLRQLQTGPDPEALTKLQHVDFSGDSLNSWKCEDD
                     PAEKEWQQVVSQAKPSSNGLVLQGFFTDIRPLDNLKNSTALYWAPLSVSAEEDERFPL
                     DCTRYPLVEITYRGLTRHARLACQWSYPGGAHLVHLETTGDWRTAALMIPLRGFPGEI
                     TRFTLRVYASTRSEESVEIARVRFRELLPEEQQTLDFYFAVSPDMSAPRKYPLLDEHL
                     PFGVSMDADTVSRLANMMDINYFDYWRLAFEDIARHHHDCVIVERMEVMTDENRSILV
                     DLAENFGLRLIPTFRWPLEQFEEKGDEWIRTYIEPHATSRGIFAWNIHDNPEEHYFKS
                     YLSARDKIAAVDTRHPVVFHSRQADTFPLYAPHFAAAGFSHFKPGDALSVKDSLRTHL
                     PLMGGQQLWITAPAFVRASGAPEWSTSPQLRMMLNMTLANGARGWMAHCYHNTPVWLN
                     GHYQRSLTGPFLTFSDLWAELGTRIERLSVMAPLFLYARPMSENNPFGIKVAVRKSVK
                     SPLAQDEDALSIFWLEGPDYYLCHLINNDAGHVTSVDLSFPDSLPDNMEIYDTTALVR
                     IRAWAQAPRRQHLEMSPGQGQLYLIAKAPVCLHWREVFARRILTADQRQTKVDLELAR
                     QYELDVAEIETTLRARDEEMSLEELHSARAAKDALFNLIYATPAIYETRELLVKASSI
                     IRGCDEAICSLHGQENIKKARKLGPKVVPYARTLTELRLRLRRGYGDEIKQEAEDLVQ
                     KSLELLHTIWHNLAT"
     gene            6994..7617
                     /locus_tag="PKN92_07270"
     CDS             6994..7617
                     /locus_tag="PKN92_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ48630.1"
                     /translation="MAQSCHISRDSYLKPVTAQEIKISALLKRAVETLHGQSGFDLSV
                     QKLAEEIPLSKRTVYKYFPGKEHLLAEAVHLDFMQWSGWFFTTLASRTEKPSEGLAAF
                     CSLLQIWAASPDYRGCLFARAAFHGDPKPGVLAESAVACTEQLHRKLSTLMTDAGADP
                     EHLLSRFFFEGVVTVLSGKPCRSHPLTPDDLIRLLRSLPVQKQKRGG"
     gene            7617..8654
                     /locus_tag="PKN92_07275"
     CDS             7617..8654
                     /locus_tag="PKN92_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ48631.1"
                     /translation="MANPVPNQRPVRRLPLPRFFQKRFRHVILHITERCTLRCRSCFV
                     KKSARDLSLEEAEILAQKLGAPRWLDIGGGEPFLHPQLPEICALFPRSDLTIPTNGQT
                     PEHIEQTVRQIAGAHRKPLTIALSLDGFEEANDLIRGKGSFARAMESYRRLRAIEGIS
                     LKINTVVCNANAGELLRFMAYVRSLDPDYHSLLLLRGVPEDNDLSLPPLSFLEEHTPE
                     ILGILGSYLYSKKDNALLRRLKKHYQRYLWQVSLKSLKEQRWLVPCQAPRLHRVIYAD
                     GSLSMCELMPSAGNLLREPMDSLEQKMKHALAAHEKAHGGCFCTHNCNMAENIMTHPY
                     SILAVLLGLKP"
     gene            8651..9577
                     /locus_tag="PKN92_07280"
     CDS             8651..9577
                     /locus_tag="PKN92_07280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ48632.1"
                     /translation="MNTVMASIIIPVYNRHGELQRLLHQLSGQTNTAFEVVLVDDGSD
                     PPINLRETETPLPFPVVLKRHTMRSGIGKARNTGVQAAGAPLLLFLDSDGDLIDHAWF
                     EKHLRLHRRAKELAAAAGKDLCVVHSEVLGTAPGWWGKVDGYSNWYGSTGRKEKAIRD
                     RHVPTHNTSVEKAIFERTGLFDETLEVLEDVEWGLRCLSSGIALYYLPGAPVRHLDRS
                     TFRSVWRHYETFGYFAPLVRKKAADATYSFLFPRGMLSALLLFLPVTGLMTLYILFRW
                     IGHRPQVLWYLPGLYIANVAYYAGICRRLREG"
     gene            complement(9626..10225)
                     /locus_tag="PKN92_07285"
     CDS             complement(9626..10225)
                     /locus_tag="PKN92_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48633.1"
                     /translation="MIELTGGQIYLMEKITMFAFPQKILLPGVVLLCVLCLAGCKPKE
                     RDDLTGKGTGDWYLQGKVILHGDVVDFQPGKKFKIQTRLISESSVPVLNKVRMAGNYS
                     INMEEYPHEIDMNINRLYFGWLPVKIPEISTLGIFKMQKKESEVTLYLNPGIRNERRP
                     MPVTLDTERGPVFVATRSLAKSQEDESGWLNRAVDLLFP"
     gene            complement(10314..11744)
                     /gene="uxaC"
                     /locus_tag="PKN92_07290"
     CDS             complement(10314..11744)
                     /gene="uxaC"
                     /locus_tag="PKN92_07290"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HNZ48634.1"
                     /translation="MNAAAFITDDFLLETEYARDLYDNFVRNLPVIDFHCHLPSELVA
                     SDHRFKNMTEIWLKGDHYKWRLMRTNGVAEAFCTGDASDREKFEKWAETVPYLLRNPA
                     YHWTHLELRKPFGITDRLLEPETAEEIWEECNSMLASPDFSARGILKQMNVALICTTD
                     DPVDDLESHSLVQEDGTMEMQMLPTWRPDKALTVDQPDLFNAWIARLEEASGISCDTY
                     EAFLEALRKRAIFFKLRGCKIADHGLEIPYSCAYNPETVSRAFSDARKGLIPDPDHVM
                     QYRSSLMYELCVLNHQMDWAQQLHLGPLRNTNTRMFNHLGPDAGYDSVGDHAMAKELA
                     RFLDRLDAQSLLTRTIIYTINPALNMMVATMAGNFQEGPVPGKIQFGSGWWFNDQLDG
                     MYDQMETLSQTGLLSRFVGMVTDSRSFLSFPRHDYFRRLLCNMLGNDMAKGLIPKKPD
                     RVGRMLVDICHQNAIRYFGFDLPGVM"
     gene            12077..13303
                     /locus_tag="PKN92_07295"
     CDS             12077..13303
                     /locus_tag="PKN92_07295"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ48635.1"
                     /translation="MNDTSPTNQFSDFDLDPRCLKVLQSAGISHSSPIQLQAIPASLT
                     GRDLIATAQTGTGKTLAFVLPGLTRLVQSRAGFNRMLVLLPTRELCQQVATVMEDYCK
                     ALNLRCVPIYGGVGMHTQTQRLQKGCEVIVATPGRLLDHLSRNHIHFKELQILVFDEA
                     DRMLDMGFLPDIRRILKTLPRQRQNLMFSATFAPALERLARDMMRNPERISAGAISKP
                     VDTVRQLVVPVRENDKLTMLLRLLKEESIDSGLIFMRTIQRTEKVGRALKGGKFKTAI
                     IHGDLPQQQRQRALEGFRQGKYQLLAATDVAARGLDIDDVSHVINYDIPENADDYLHR
                     IGRTARAAQEGDAITLVAPSDHAALAAIEETLGYNIERRNYEGAPKILTLYRPQDAPA
                     PAAAKRRRGRSLLRRR"
     gene            13359..14486
                     /locus_tag="PKN92_07300"
     CDS             13359..14486
                     /locus_tag="PKN92_07300"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNZ48636.1"
                     /translation="MVKYRVASYYFPNYHPDKRNQERLGDGWTEWELVKQAKPRFYGH
                     NQPKQPLWGYTDESDPIQMAQKIDAAADHGINTFIFDWYFYNDGPFLERGVEEGFMKA
                     ANVQRLEFALMWANHDWVDIFPARYSDESKLLYPGGVTAETFDRMTDVLIEKYFSHPS
                     YWRIDGCPYFSLYELHTLIQGFGGVEETAAVFKRFREKAVAAGLPGVHLNAVAWGVQI
                     LPNEKVIKEPEQLIEAIGFDSVSSYVWVHDVHLTRFPQVPYGDVLEKILPVWERNSTR
                     FGVPYFPNVTMGWDSSPRCHGDDPYINRGYPFMPSFSGNTPEMFRKALEEVRAFLDSH
                     PQCQNSFSINCWNEWTEGSYLEPDVEHGLAYLEAVKKVFGA"
     gene            14519..15568
                     /locus_tag="PKN92_07305"
     CDS             14519..15568
                     /locus_tag="PKN92_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48637.1"
                     /translation="MNTGAAAKSSSEAAEPLLKVSGLTVLFQTDQGTARAVDGVSFAL
                     HSGETLALVGESGCGKSVTALSLLRLIPSPPGRIASGSVVFDGKSLLELPEKAMRDLR
                     GNDISMVFQEPMTSLNPVFRIGDQMTAVLRLHQQLSRSEARQASVALLRQVGIPEPEQ
                     RMDDYPHQLSGGMLQRAMIALALACAPKLLIADEPTTALDVTIQAQILQLLRELQTES
                     RLALLLITHDLAVVAETADRVAVMYAGRIVETATVEALFNEPRHPYTQGLFASLPGKE
                     KQGDRLSAIPGTVPPATHFPAGCRFHPRCDKAMPLCRRAEPALHPSGASHQTACWLYQ
                     PHPDADEEGKDLWRH"
     gene            15556..>15757
                     /locus_tag="PKN92_07310"
     CDS             15556..>15757
                     /locus_tag="PKN92_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ48638.1"
                     /translation="MAPLISVQGLIKHFPIRTGVLSRVTGAVHAVNNISFSIDRGTTL
                     SLVGESGSGKTTAGRCLLRLIEP"
     assembly_gap    15758..15767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15768..16527
                     /locus_tag="PKN92_07315"
     CDS             <15768..16527
                     /locus_tag="PKN92_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ48639.1"
                     /translation="DGEDVLRADSARMRELRKRMQIIFQDPFGSLNPRMTVHAMLREM
                     LLFHGIVPRSGLNRQIALLLDRVGLPASAGDRYPHEFSGGQRQRLGIARALAVEPEFI
                     VADEPVSALDVSIQAQILNLLQDLQESLGITFLFIGHNLDVVRHISTHVAVMYLGHIV
                     ETAPVSALFEDPVHPYTLSLLAAVPVAKVTKEKRRIIIPGEVPSPINPPSGCPFHPRC
                     PLAEEECKTRKQHLEPLSPEHQVACAVQMRKRAN"
     gene            complement(16549..18069)
                     /locus_tag="PKN92_07320"
     CDS             complement(16549..18069)
                     /locus_tag="PKN92_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNZ48640.1"
                     /translation="MKRRDFLKAAAFSTMLTSCVGHETVSHHPEAPRQNFVFILADDL
                     GWTDLGCFGSSFYDTPCLDRLAGEGMRFTDAYAACPVCSPSRVSIMTGKYPARLHTTD
                     WFGAPQPDTVENHPTRDKALLPAPYVEQLPLSEVTLAEAFKEAGYRTFFAGKWHLGGK
                     GFYPEDQGFDINKGGYEKGSPNSYYSPYQNPKLTDGPEGEHLPRRLAEESVAFLEKNG
                     KKPFVLFLSFYSVHTPLKGREDLVRKYEARAKEQNQEQAVYKEIDGRQVRQVQNDPQY
                     GATVEAMDESVGMVLGALNRLGLSESTAVFFTSDNGGLSTSEGTPTSNVPLRAGKGWL
                     YEGGIRVPLIVRWPGHTAAGSVSSRPVAGTDFYPTMLEIAGLPARPHQHCDGMSVVPC
                     LQGKNSKEERPLFWHYPHYGNQGGSPGAAVRMGRYKLLEFFEDNRIELYNLQEDPGET
                     ENLADEMPNYADAYLELLHDWQQECGARFPSPNPKAAAQPESSGKKKISPKSIYHK"
BASE COUNT         4227 a         5125 c         4900 g         3947 t
ORIGIN      
        1 gcacggggaa accagaaagg aacgcagtca tccagcagca ttttccggta ctgctccgca
       61 agattctgca gccggtgatc ttccataaaa aacagccttt ctcctgtttc aggcatccgg
      121 gtccaatgta tcatcgagag tgtacaaagt cagtccgcgc atggatcgct ccgaacggga
      181 aaacagcaaa ctggcagcat aacctgtaat aaaacagaat acgatgcccg ccacagcata
      241 gagcagaaaa tgggcggcgg taaagcgctg caccagaaaa aggcccgctg ctccggcgag
      301 agcgccgatc atggcgccag gtgcatttgc ccgggtcgta aaaataccga gacagaaaag
      361 accgcacatg ggtgcgccga aaagtccaag aatggtcatg aactgatccc acaaagacat
      421 gatgcccgaa gaggcgaaaa gcaatgccag cccgacaccc gccgcaccga aaagaaccgt
      481 gagtagacgc cccagccgca gaagagccga ctccgtcttc agcagacgcc agggctttat
      541 aaaatcctcg actaccgctg tggacatgct gttgatgctt gtggaaatgg tggattgggc
      601 agcggcaaaa agccccgcta ccaccagtcc tgacactccg gcgggcagct cccgggcaat
      661 aaaaagcgga aaaatggcat ccgttttaaa cgtgggatca agccgctccg gatgctgcct
      721 gtaaaaaaca aaaagggctg ttccgatgcc gaagaaaagc aaggaagacg gaatacatgc
      781 cagtgcattt gtccagatcg ctttttttgc ccgggcagta tccggcaccg acatatacct
      841 ttggacaata gacatgtcgg agctgtagga aatcagattc tgcgccatgc cccccagaag
      901 cacaacccac aacgccgaag tcataaaact ggacacggaa aaatccgcct gaaacagaac
      961 cagttttcca tgcgcatgcg ccgtttgaaa aaatccgaag agccctccgt caatatagat
     1021 cagcatcaga atcaggctga acagagcacc gcccatgagc acggcgctct gaatggcatc
     1081 cgtccagaca accgcctcca gcccgcccag cgtgcagtag acaatactca ggacacccat
     1141 caaaagaatg ctctgctgtt ctgtcagcgg ggtgatcgtt cgcagcgcca gcgcaggcag
     1201 atagagcaca acacccatgc ggcccagctg aaaaaagaca aaagaggcgc tggcaaactg
     1261 gcgtatgaag cggttgaatc gtttttccag ataatgatag gcgcttacag catcgacttt
     1321 acgaaagaag ggcaggaaca gatatatgac cagcggtgcc gaagcgacta cggtcatatt
     1381 aaagaggaaa aaaatccagt tcgtggcata actttttgcg gggatcgcca tgaaggttat
     1441 ggaactgagc atggtcgcaa aaatactcag cccggcgaca aaccagggaa cacgctgtcc
     1501 gccccggaaa aaatcgtccg tgtttttgtt gcgaaaagaa aagaagacac ccaaaagcac
     1561 catagcggcg agataaatgc caagcattac aaagtccagt gtgccgaaac gactggcgcc
     1621 gggaacaggc cgtactgccc acacctgcgg cgtcctgaca cggggacgaa tctcaccgct
     1681 gatcataaga acagggtcac ggactgtatc ctgtccccac ctgacggcgg gcgtggtcac
     1741 ctgatttgcg ggtgtggttc cggccgccgt ccaggtgtcc gtaatggtgt gataggcaaa
     1801 ggcttccttc ggaaaacccg gatgcgcatc tttcaggagg tcagtctgat agaaaagcgt
     1861 ttcatcggct ccgctcagca caaaaatgtg gctctgcccc agagcgattc cggtgcccgc
     1921 catgatgcag cggggcgtat cagtgcgata gtgccagggc tccgccctgc ccggcatata
     1981 ttcgtacaca tccctcagaa attccgtgac cccttccccg tttacccggc gtccgctcat
     2041 gacatagacg cagtccgcaa caccgttatg ctgtgccgcc actaccgcaa gcgcacggga
     2101 aggtccgggc cagggaggca gctcctccca gcggaatcca tcggtaccga aaagagacag
     2161 atccagcatc cagaaattcc gctgcgccgt ttccagttca agaccggtac agccgctcgc
     2221 cacatagacc cgatcccgga tcacagcggc agtactgttg gtgcagggcg caggcagagg
     2281 cggcaggggc tgcatgacaa cgctttgttc ctgttcctgc cactgcatca gaaaggcatc
     2341 gcgaaagcac tcatcagcat cattgccgcc cagacagaca acgccgcggc tcgtggaagc
     2401 ggaggcgcca taggcaacgg gccggggcag tttaccgccg tgaatccatc ggcagtctgt
     2461 cccttccctg ccgatgagaa cgtaaatatc gtcgtgaaat acttttggtg attcccatac
     2521 gggctcggaa aaattggcgc cgccggctgc aatcacagca ttgttatgaa atcctgcgaa
     2581 aatgcctgct accccatcgg gattcggaag cggaggcagg gcagcccagt caagaggtat
     2641 cgtggctcca gctgcagaag caacaaggag cagtatgact gcaagaaccc tgccctgttt
     2701 tcgtaacagc atttttctat ccaatcccct tcggaaatcc cagaacctgc agttcttttt
     2761 ccagcgcgct gtgctcgtcc ggacgcagcg gtgtaagcgg aagacgtaca ggaccgcagg
     2821 gaatgcctga taaagacata gccgctttca gtcccgccag accgtgatag cgaaggatga
     2881 cttctataat ccccgccgcc tgcagctggc agcgctgcgc cgcttccaga tccccttcgc
     2941 tcaaatgacg catcactttt tggtagaggg ggctcagaaa attgtaggtg ctgccaactg
     3001 caccgtgtgc tccggcgaca agtccgctca gaagcgtttc gtccacgccg aagaggatgt
     3061 tgtatctccc gccgccaaaa gcaagacact gctgcatgac ttctatttca cgggcactga
     3121 acttgatccc tgccagcgtg ggcacgcggg agggcgcctg tttcagaaat tccatcatgt
     3181 tgacggaaag cccgctcagc ttgggaatgt ggtaatagta aaaaggcagc tcaggcgccg
     3241 catccgtgat gtgtcccagc gtttccacga ggacggcagt gctttccggc aggtaatacg
     3301 agggagacac cgcactgatg gcatcagcac cgatctcggc agcatgcgcc gccagcgccc
     3361 gcgcctcttc aatggagttg tgccccacct gcacaattac aggaatacgc ccgcccgcag
     3421 cttccacata ggcacgggca accgccttcc gttcttctgc agtaagagac ggcccctccc
     3481 ctgtgctgcc gcagacataa agcccagtaa tgccccggct gatgagtcta tccacgatag
     3541 tttcgacagg cttcagatta atctgcgcct ggtctgtcat gggagtgaag gtcgccgcaa
     3601 tcaatccggt aagtttcatc atatttttta ttccttagcc cgctattaat gtctcttatt
     3661 tcctcctggc gtgtcaactt taccggcgat ctgcaccccc ctatcggcat ggaacggcat
     3721 ccgctgtatt tttcacaggg caagcaggta caatgatgag gcgggcaatc caatccgcaa
     3781 gagattttcg gcaccgcatt tcactgtaaa atcaggagtt gaaatcaaat gaaaccttcg
     3841 ctgcttttag tgcttgtttt gggcgtttct atttttaccg gctatgcggg cgcagaagaa
     3901 cttcgggtgg gcatactcgc agatgcatca tcggatttct ggacaaagct gcgcgatgaa
     3961 gcacagactg cagccaaatc gctggacatg acactggatt tccgggtacc tgcaccgccc
     4021 acagcggaac agcaggaagc actggcgcaa aaaatgatcg acgccggcgt gaaggctctg
     4081 gcgatctgcc cggcaaaagc ggccaagcag aaagactttc tggaagcgct ggcgcagcgc
     4141 ataccggtca tcgtcctttt taaggaagtg ccgaacagca ccgatcttgc tttcctcgga
     4201 aaagacaaca gggaaagcgg aagactgctc gccacactga tccgggaagt tgttcccgaa
     4261 ggactcaaga tacaggtgct ctgcgatgac cagacccggc ctgccacaca gcagcggatt
     4321 gaaagttttc gtgaagcgtt gggcagctat gccattgtag atgcggtgct ggaagacaag
     4381 ggagaccgca tgctcggctg ggcaaacgtt atcgacagcc ttgagagccg ccctgaaatt
     4441 gccgcctttg tgggcatgca ggactatcat ggcccgatga ttctgcgcgc tgttctcgaa
     4501 aaaaaccggg gaaaatgggt acgcgtggtg agctgcggaa gtgatccctc cacccttaaa
     4561 ggtcttgaag atggtaccgt ccatggactg gttgtcgatg atgcggccgg gtcggcacag
     4621 gcaatagtca aggggcttgc cgcctttgca cgaaatgata aggaaggaat tgaagaaaca
     4681 aaagtggcta ccattcctgt taccattctc aaaacagaag gtaccctgtc accagaggat
     4741 atgatgaatg ctctccaatt acagttacca tgggtgtctg aggcaactcc agacaggccc
     4801 tgacccggaa gctcttacaa agctgcagca tgttgatttt tccggagaca gcctgaacag
     4861 ctggaaatgc gaagacgatc cggcagaaaa agaatggcag caggtggtgt ctcaggcgaa
     4921 gccctcttcg aatgggctgg tgcttcaggg cttttttacg gatatccgtc ctctggacaa
     4981 tctgaaaaac agcactgccc tctactgggc gcctctctcc gtttcggcgg aggaagacga
     5041 gcgctttcct ctggactgta cccggtatcc gcttgtggaa atcacctaca gaggtctgac
     5101 ccggcatgcg cggcttgcct gccagtggag ctaccccggc ggcgcgcatc tggtacatct
     5161 ggaaacgacg ggcgactggc ggacagcggc gctgatgatc ccgctgcgcg gattccccgg
     5221 cgaaatcacc cgattcactt tgcgggtcta tgcatccaca cgcagcgaag aatccgtgga
     5281 aattgctcgg gttcgtttcc gtgagctcct cccggaagaa cagcagacac ttgattttta
     5341 ctttgctgtt tcgccggaca tgtctgcgcc cagaaaatat ccccttctgg atgagcacct
     5401 gcccttcggc gtctccatgg atgccgatac agtcagccgc ctggcaaaca tgatggacat
     5461 caattatttt gattactggc ggctcgcctt tgaagacatt gcgcgccatc atcatgactg
     5521 cgtcatcgtg gaacgcatgg aagtgatgac cgatgaaaac cggagtattc ttgtggatct
     5581 tgctgagaac ttcggtctgc gcctgatccc gactttccgc tggccgctgg aacaatttga
     5641 agaaaaaggc gatgagtgga tccgtacata tatcgagccc catgcgacat cccgtggcat
     5701 ttttgcctgg aacattcatg acaatcccga ggaacattat ttcaagagtt atctgagcgc
     5761 ccgggataaa attgcagcgg tggatacacg ccatcccgtg gtgtttcact cgcgccaggc
     5821 agacaccttt cccctgtatg cccctcactt tgcggcggcg ggcttctctc atttcaagcc
     5881 gggcgatgcc ttgagcgtaa aggactcgct tcgcacccat ttgccgctca tgggcgggca
     5941 gcagctgtgg atcaccgccc ctgcgtttgt ccgggcttcg ggcgcccctg aatggagtac
     6001 cagtcctcag ctgcggatga tgctgaatat gaccctggct aacggcgcac ggggctggat
     6061 ggcacactgc tatcacaata cgccggtgtg gctgaacggt cattaccagc gttcgctcac
     6121 cgggcctttt ctgaccttca gcgatctctg ggcggaactg ggcaccagaa tagagcggct
     6181 gagcgtgatg gcgcctttat ttctgtatgc gcgtcccatg agcgaaaata atcccttcgg
     6241 cataaaagtg gcggtgcgca aaagcgtgaa atcccctctg gcgcaggacg aggatgctct
     6301 gtccattttc tggctggagg ggcccgatta ttacctctgc catctcatca acaacgacgc
     6361 cgggcatgta acttctgtcg atctgagttt tcccgattct ttaccggaca acatggaaat
     6421 atacgataca acggctctgg ttcgtatccg tgcctgggca caggcaccgc gcaggcagca
     6481 tcttgaaatg tctccgggac agggacagct gtaccttatc gcaaaggcgc cggtgtgcct
     6541 gcactggcgt gaagtattcg cacgccgcat cctgacagcc gaccagcggc agaccaaagt
     6601 ggatctggag ctggcgcggc aatacgagct ggatgtggcg gagatcgaga cgacactgcg
     6661 tgcacgggac gaagagatgt cgctggaaga gcttcacagt gcccgggcgg cgaaagatgc
     6721 cctgttcaat ctcatctatg ccacccccgc catttacgag accagagaac tgctggtgaa
     6781 agccagttcc attatccggg gctgtgacga agcgatctgc tcgctgcacg gtcaggaaaa
     6841 catcaagaaa gcacgaaaac tgggcccgaa agttgttcct tatgcccgca ctctgacgga
     6901 gctgcgcctg cgcctgcgcc gcggctacgg agatgaaatt aaacaggaag cggaagatct
     6961 ggttcagaaa agtctggagc tgctgcatac catatggcac aatcttgcca cataagccgt
     7021 gacagctatc tcaagccggt aacagcgcag gaaatcaaaa tcagtgcgct cctgaaaagg
     7081 gctgtagaaa cactgcacgg gcaaagcggc tttgatctgt ctgtccagaa gcttgccgaa
     7141 gaaatccctc tttccaaaag aactgtttat aaatattttc ccggcaaaga gcatctgctg
     7201 gctgaagcag tacacctcga ttttatgcaa tggagcggct ggttcttcac cactcttgcc
     7261 agccggacag aaaaaccgtc tgaaggcctg gccgcttttt gcagcctgct ccagatatgg
     7321 gcagcctcac cggattaccg gggatgcctc tttgcacgcg ctgcttttca tggggatccg
     7381 aagccgggtg tgcttgctga gagtgcggta gcgtgtacgg agcaattgca ccggaaattg
     7441 tcaacgctga tgacagatgc aggcgccgat ccggaacacc tgctgtccag attctttttt
     7501 gaaggcgttg tcaccgtcct gagcgggaaa ccctgcaggt ctcatccgct cacgccggat
     7561 gatctgatca gactgctgcg cagccttcct gtacagaagc agaaaagagg cgggtaatgg
     7621 cgaaccctgt tccaaatcag cgtcccgtcc ggaggcttcc gctgccgcgc ttttttcaga
     7681 aacggttccg ccacgtcatt ctgcatatta cagagcgttg tacgcttcgc tgccggagct
     7741 gctttgtgaa gaaaagtgcg cgggatctca gtctggaaga agcggaaata ctggcacaaa
     7801 aactgggagc gccccggtgg ctggatatcg gcggcggcga accttttctg catccgcagc
     7861 ttcccgaaat ctgcgctctt ttcccccgat ctgatctgac cattcccacc aacggccaga
     7921 caccggagca cattgaacaa acagtcagac agattgccgg agcgcaccgg aagccgctga
     7981 ccattgcgct ttcgctggat ggttttgaag aagcgaacga cctgatccgg ggcaaaggca
     8041 gctttgcccg tgcaatggaa agttacaggc ggctccgtgc cattgaaggg atcagcctca
     8101 aaatcaatac agtggtctgt aatgccaatg ccggtgaact cctgcgtttt atggcatatg
     8161 tccgcagtct ggatccggat tatcattcgc tgctgctgct ccggggtgtt cccgaagaca
     8221 atgacctgtc gcttccgccc ctttcttttc tggaagaaca cacccctgaa atcctgggca
     8281 tactcggctc ctatctctac agcaaaaaag acaatgcgct gctgcgacgg ctgaaaaaac
     8341 actatcagcg gtacctgtgg caggtatccc tgaaaagtct gaaagaacag cgctggctgg
     8401 ttccgtgcca ggcgccccgg ctgcatcgtg tcatctatgc cgacggcagc ctgtccatgt
     8461 gcgagctgat gccttctgcg ggaaacctgc tccgggaacc gatggattcc cttgagcaga
     8521 aaatgaagca cgctcttgct gcccatgaaa aagcgcacgg cggctgtttc tgcacacaca
     8581 actgcaacat ggctgaaaac attatgaccc atccctactc gattcttgct gtgctgctgg
     8641 gattgaagcc atgaacacag tcatggcatc cattattatt cctgtgtata accggcacgg
     8701 ggagctgcag cgtcttctgc accagctgtc cgggcagacg aatactgctt tcgaagtggt
     8761 tcttgtggat gacggctccg acccgcccat taatcttcgg gaaacagaaa cgcccctccc
     8821 ctttcctgtt gtgctgaaac gccacacaat gcgcagcggt atcggcaagg cgcgcaatac
     8881 aggcgtacag gcggcaggtg cgcccctgct gctttttctg gacagcgacg gagatctgat
     8941 agatcatgcc tggtttgaaa agcatctgcg gctgcatcgg cgtgcgaaag aactggcggc
     9001 tgctgcgggc aaggatctgt gtgtcgtcca cagtgaagtt ctgggtacgg cgccgggctg
     9061 gtggggcaag gtggatggct attccaactg gtacggctct accggcagaa aagaaaaagc
     9121 catccgggac cgtcatgtac ccacgcataa caccagcgtc gaaaaagcga tatttgagcg
     9181 cacaggcctt ttcgatgaaa cgctggaggt gctggaagat gtggaatggg gtctccgctg
     9241 cctgagctcc ggcatcgctc tttattatct gccgggcgca ccggtccgcc atcttgaccg
     9301 cagcactttc cgaagtgtgt ggcggcacta tgaaactttc ggctactttg caccgctcgt
     9361 ccggaaaaaa gctgccgacg caacctattc cttccttttc ccccggggaa tgctgtccgc
     9421 tcttttgctt ttcctgcccg tgaccggcct catgacgctg tacatcctgt tccgctggat
     9481 cggtcaccgt ccgcaggtac tctggtatct gcccggcctt tacattgcca atgtggcgta
     9541 ttatgccggg atctgccgac ggctcagaga gggctgaacg ctttcccgct gttacttccg
     9601 ggagcacagg aagtaacggc cggaatcaag ggaagagcag atccaccgcc cggttcagcc
     9661 agccggattc atcttcctgc gatttggcga gagatcttgt tgccacaaaa acgggccctc
     9721 tttctgtgtc cagcgtcaca ggcatgggcc ggcgttcatt tcgtatgcct ggattcagat
     9781 acagggtgac ttcgctttct ttcttctgca ttttaaaaat acccagcgtg gaaatttcgg
     9841 gtattttgac agggagccag ccgaaataaa gtctgttgat attcatgtct atttcatgag
     9901 ggtattcctc catattaatg gagtagttcc ccgccatcct caccttattg agcacgggaa
     9961 cagaagattc tgaaatcagc cgtgtttgaa ttttaaattt tttgcccggc tgaaagtcca
    10021 ccacatcacc atgcaaaatc accttcccct gcagatacca gtcacccgtc cctttcccgg
    10081 tcaggtcatc gcgctctttc ggcttgcagc ccgccagaca taaaacgcac aggagtacga
    10141 cgcccggaag cagtattttt tggggaaagg caaacatggt gattttctcc atcaggtaaa
    10201 tctgaccgcc agtcaattct atcacaaccc ttttcttttg aaaagagcaa caaaaatccc
    10261 ggacagatac ggcaagcctg tccgggacag aattcatgaa tgcgggcgga gaactacatc
    10321 acgccgggca aatcaaaacc gaaatagcgg attgcattct gatggcaaat atccaccagc
    10381 atcctgccga cacggtcagg cttcttcgga atcagccctt ttgccatgtc atttccgagc
    10441 atattgcaca ggagccgacg gaaataatca tggcggggaa agctaaggaa actccggctg
    10501 tccgtgacca tgccgacgaa gcgactgagc aatcccgtct gcgagagggt ctccatctgg
    10561 tcatacatcc cgtccagctg atcattgaac caccatccgc tgccgaactg aatctttccg
    10621 ggcacaggtc cttcctgaaa gttgcctgcc atagtcgcaa ccatcatgtt gagggcagga
    10681 ttgatggtgt aaatgatggt gcgggtcagc aggctctgcg catccaggcg atcaagaaag
    10741 cgcgccagtt ccttcgccat ggcatgatcg cccaccgaat cataaccggc atcaggcccc
    10801 agatggttga acatgcgggt attcgtattg cgaagaggtc cgaggtggag ctgctgcgcc
    10861 cagtccatct gatgattcag cacgcaaagc tcatacataa gggaggagcg gtactgcatc
    10921 acatgatccg gatcaggaat gagccccttc cgggcatcgg agaaggcacg ggaaaccgtt
    10981 tccggattgt acgcacagga ataggggatt tccagaccgt gatccgcaat cttgcagccc
    11041 cggagtttaa agaaaatcgc acgcttgcgc agcgcttcca gaaaagcctc ataggtatca
    11101 caagatatac cggatgcctc ttccagacgg gcaatccagg cattgaacag atcgggctga
    11161 tccacagtaa gcgctttatc agggcgccag gtcggcagca tctgcatttc catggtgccg
    11221 tcttcctgta ccagactgtg cgattccaga tcatccaccg gatcgtccgt ggtacaaatc
    11281 agggcaacat tcatctgctt cagaataccc cgggcggaaa aatccggcga tgcaagcata
    11341 ctgttgcact cctcccagat ttcttctgct gtttccggct caagaagccg atccgtaatg
    11401 ccgaaaggtt ttctcagttc gaggtgcgtc cagtggtacg ccgggtttct tagtaagtaa
    11461 ggtacggtct ccgcccactt ttcaaatttc tcacggtcac tcgcatcgcc ggtacaaaac
    11521 gcttccgcaa caccattggt gcgcatgagc cgccatttat agtggtcgcc cttgagccag
    11581 atctccgtca tatttttgaa gcggtgatcg cttgcgacca gttccgaagg cagatggcaa
    11641 tgaaaatcga taacgggcag attgcggaca aaattgtcgt ataaatcccg ggcatattcg
    11701 gtttcaagca gaaaatcatc tgttataaaa gcagctgcat tcatcgtcat agaaatactc
    11761 cttctgtcgg ataacccttc agcctgacat tggccaccca cggcaaaacc agcccaaacc
    11821 gtcccgactt gattctgacc caaaaagaca gaataccacg tttttcacaa aaagacaagt
    11881 aaagagaaag ttttttcagc aggcactgcc tcataaaaga gtgcgcctcg caggtcaaaa
    11941 aatgtgcgtt tctcttttct tttttcctgc agcccccctt ttcaacgaaa acagaccgaa
    12001 ggctttaccg ggctcatgct taaaataaat tccatctttc gcattacaga ggcaaatagt
    12061 ttataatgag gctcttatga atgacacatc ccctacgaat cagttttccg acttcgacct
    12121 tgacccccgg tgcctgaagg tacttcagag tgccggaatc agccattcct cccctattca
    12181 gctgcaggca atcccggcat cgctgacggg gcgtgacctg attgcgactg cgcaaacagg
    12241 tacaggcaaa acgcttgcct tcgtgctgcc cggtctgacg cggctggtgc aatcccgtgc
    12301 gggctttaac cgcatgcttg ttctcctgcc cacccgcgaa ctctgccagc aggtagccac
    12361 cgttatggaa gactactgca aagcgctgaa cctgcgatgc gttcccatct acggcggtgt
    12421 gggcatgcac actcaaacgc agcgacttca gaaaggctgc gaagtgattg tagccacacc
    12481 cggtcgcctg ttggatcatc tgtcccggaa tcacatacac ttcaaagaat tgcagattct
    12541 ggtattcgat gaagccgacc gcatgctgga catgggtttt ctgcccgata tccgccgaat
    12601 tctgaaaaca ctgccccggc agcgacagaa tctgatgttt tccgccacct ttgctcctgc
    12661 actggaacgg ctcgcccggg atatgatgcg taatcccgag cggatcagtg ccggagccat
    12721 cagcaaaccg gtggacacag tacggcagct ggttgttcct gtccgtgaaa acgacaaact
    12781 gaccatgctg ctccgccttc tgaaagagga atccattgat tccggcctga tttttatgcg
    12841 caccatccag cgtacggaaa aagtgggcag ggcattgaag ggcggcaaat tcaaaacagc
    12901 catcatccac ggcgacctgc cgcaacagca gcgccagcgg gcgttggagg ggttccggca
    12961 gggcaaatac cagctgctgg cagccacgga cgtcgctgcg agaggccttg atatcgatga
    13021 cgtatctcat gtgatcaact atgatattcc ggaaaatgca gatgattatc tgcaccgaat
    13081 cgggcgcacc gcccgtgccg ctcaggaagg agacgcgatt acactggtcg ccccctccga
    13141 tcacgccgca ctggctgcca tcgaagagac gctgggctat aatattgaac ggcgcaacta
    13201 tgagggtgcc ccgaaaattc tgactttgta ccggccgcaa gacgcaccgg cccctgcagc
    13261 agcaaaacgt cggcgcgggc gcagcctttt gcgcaggcgc tgaacccgcc tgccggtatt
    13321 tccacgatta aaccattcat gcactgaagg aattcatcat ggtaaagtac agggttgcaa
    13381 gttattactt tcccaactat cacccggaca aacgcaatca ggaacggctg ggcgacggct
    13441 ggactgaatg ggaactggtg aaacaggcaa agccgcgttt ttacgggcac aatcagccca
    13501 agcagccact ctggggctat accgatgaat cggatccaat ccagatggcg cagaaaatag
    13561 atgcagcggc ggatcatggc atcaatacct ttatttttga ctggtatttc tacaacgacg
    13621 gcccttttct tgagcggggc gtggaagaag gcttcatgaa agccgccaac gtgcagcgcc
    13681 tggaatttgc gctcatgtgg gcaaaccatg actgggtcga tatctttcct gcacggtaca
    13741 gcgacgagtc caaactgctt tatcccggcg gagtcaccgc tgaaaccttc gaccgtatga
    13801 cggatgtgct gattgaaaag tatttctccc atccctccta ctggcgtatt gacggttgtc
    13861 cctatttcag tctctatgaa ctgcatacgc tcattcaggg cttcggcggc gtggaagaga
    13921 cagcagccgt cttcaaacgc ttccgtgaga aggcagtggc cgccgggctt cctggagtgc
    13981 atctgaatgc ggttgcgtgg ggcgtgcaga ttctgcccaa tgaaaaagtg atcaaagagc
    14041 cggaacagct cattgaagcc atcggtttcg atagcgtcag ctcttatgtc tgggtacatg
    14101 atgtacattt aacccgcttc ccccaggtgc cctacgggga tgtgcttgaa aagattctgc
    14161 ctgtatggga aagaaacagc acccgattcg gcgtccctta cttccccaac gtcaccatgg
    14221 gctgggattc ttctccccgc tgccacggcg atgacccgta cattaacagg ggctatccct
    14281 tcatgccgtc tttttccggg aacacgcccg aaatgttccg gaaagcactg gaagaggtgc
    14341 gtgccttttt ggacagccat ccgcaatgcc aaaacagctt ttccatcaac tgctggaacg
    14401 agtggacaga aggcagttat ctggaaccag atgtagagca cggcctggcg tatctggaag
    14461 cagtcaaaaa agtattcggc gcatgaacaa tgcctccaca aagtaagtgc tcagaatcat
    14521 gaatacaggt gctgcagcca aatcttcatc ggaagctgcc gaaccgctcc tgaaggtatc
    14581 ggggctgact gttctttttc agactgatca ggggactgcc cgggcagtgg atggagtctc
    14641 ctttgcactg cacagcggcg aaacgctggc actggtgggt gaaagcggat gcgggaaaag
    14701 cgtgactgcc ctgtcgctgc tccggctgat cccttctccg cccgggcgca tcgcttcggg
    14761 ctctgttgtt tttgacggaa aatcgctctt ggagcttccg gaaaaagcca tgcgcgacct
    14821 gcggggaaac gacatcagca tggtttttca ggagcccatg acttctctca accctgtttt
    14881 ccggataggc gatcagatga cggccgtgtt gaggctgcat cagcagcttt cccgcagcga
    14941 ggcgcgccag gcctctgtcg ccctgctccg gcaggtgggt attcccgagc cggaacagcg
    15001 gatggacgac tacccgcatc agctttccgg cggcatgctg cagcgggcga tgatcgccct
    15061 tgcgctcgcc tgtgcgccga aactgttgat tgctgatgag ccgaccacgg cgctggatgt
    15121 caccattcag gcgcagatcc tgcagctgct gcgggagctg cagaccgaat cccggcttgc
    15181 actgctgctg atcacccatg atctggcggt ggtggcagaa acagcagacc gggttgcggt
    15241 tatgtatgcc gggcggattg tggagactgc gacggtggag gcgctcttta atgagccgcg
    15301 ccatccttat acacagggac tcttcgcctc gcttccggga aaggaaaagc agggcgaccg
    15361 cctgagtgct attcccggga cggtgcctcc ggcgactcat ttccctgcgg gctgccgctt
    15421 tcatccccga tgcgataaag ccatgccctt gtgccgccgt gcggagcctg cccttcaccc
    15481 ttccggcgca tcccatcaga cagcctgctg gctgtaccag cctcatccgg atgcagatga
    15541 agaagggaaa gacttatggc gccactgatt tccgtacaag ggctgatcaa gcacttcccc
    15601 attcgcaccg gcgtactctc ccgtgtcacc ggagcggtac atgccgtcaa caatatctct
    15661 ttttccattg accggggtac gacactgagt ctggtcggag aaagcggcag cggcaaaaca
    15721 accgccgggc gctgtctcct gcggctgatc gagccggnnn nnnnnnntga cggagaggat
    15781 gtgctgcgag ccgactccgc ccggatgcgg gaactgcgca agagaatgca gattatcttt
    15841 caggatcctt tcggatcact gaacccccga atgaccgttc acgccatgct tcgggaaatg
    15901 ctccttttcc acggaatcgt gccccgcagc gggctgaacc ggcagatcgc cctgcttctc
    15961 gaccgggtgg ggctgccggc gtctgcaggc gaccgctacc cccatgaatt cagcggcggg
    16021 cagcgtcagc ggctcgggat tgcccgtgcg cttgctgtcg agccggaatt cattgttgcc
    16081 gatgaacctg tctcggcgct cgatgtatcc attcaggcgc agattctgaa tctgcttcag
    16141 gatctgcagg agagcctggg catcaccttt ctttttatcg gccacaatct tgatgtggtg
    16201 cgccacattt ccacacatgt cgctgtgatg tatctggggc acattgtgga gactgccccg
    16261 gtctccgccc tcttcgaaga tccggtgcat ccgtacacgc tgagccttct ggcggcggta
    16321 cctgttgcaa aggtcacaaa agaaaagcgg cgtatcatca taccgggcga agtgcccagc
    16381 cccatcaacc cgccttcagg atgtcctttc cacccgcgct gccccctcgc ggaagaagag
    16441 tgcaaaacac ggaagcagca tctggaaccc ctttctccgg aacatcaggt cgcctgtgcc
    16501 gtgcagatgc gcaaacgggc aaactgatcg ggtatcgcct gtgccccttt atttatgata
    16561 gatgctttta ggcgagattt tcttttttcc agacgattcc ggttgtgccg ctgccttcgg
    16621 gttaggcgaa ggaaagcgtg ccccgcattc ttgctgccag tcgtggagca gctccagata
    16681 ggcgtcggca taattgggca tttcatcggc aaggttttct gtttcgcccg gatcttcctg
    16741 cagattatac agttcaatcc ggttatcctc gaagaattcc aggagtttgt accgacccat
    16801 ccgtactgcc gctccgggcg atccgccctg attaccgtag tgcgggtaat gccagaagag
    16861 ggggcgttct tccttgctgt tcttcccctg cagacagggc acaacactca tgccgtcgca
    16921 atgctggtgc ggtcgggcgg gaagccctgc tatttccagc atagtcggat aaaagtccgt
    16981 gccggctacc ggtcttgatg aaacgctgcc ggcagcggta tgacccggcc agcgcacaat
    17041 cagaggaacc cgtatcccgc cttcgtagag ccagcctttc cccgcacgca ggggaacatt
    17101 gctggtcggc gtgccctcgg aggtggaaag cccgccatta tcggaagtaa aaaacaccgc
    17161 tgttgattcc gacagtccga gccggttcaa ggctccgaga accatgccga cggattcgtc
    17221 catggcttcc acggtggcgc catattgcgg atcattctga acctgacgga cctgtctgcc
    17281 gtctatttcc ttgtaaacgg cttgctcctg gttttgttct tttgcccgcg cctcatattt
    17341 tcgcacgaga tcctcccgac ctttcagcgg tgtatgtacg gaatagaagg aaagaaacag
    17401 aacaaagggc tttttgccgt tcttttccag aaaagcgacg ctttcttccg caagtcgtct
    17461 ggggaggtgt tccccttccg gcccgtccgt caacttcggg ttctgatagg gcgagtaata
    17521 ggaattgggg cttccttttt catagccgcc tttgttgata tcaaagccct ggtcttccgg
    17581 ataaaagcct ttgccgccca gatgccattt gccggcaaaa aacgtgcggt agcccgcttc
    17641 tttgaaggct tcggcaaggg taacctcgct caggggaagc tgctccacat aaggcgccgg
    17701 aagaagcgct ttgtcccggg tcggatgatt ttccactgta tcgggctggg gtgcgccaaa
    17761 ccagtccgtt gtatggagcc gtgcagggta cttccctgtc atgatactga cacggctggg
    17821 actgcacacg ggacaggcgg cataggcatc agtaaagcgc atcccttccc cggcaaggcg
    17881 gtcaaggcag ggtgtatcgt agaaactgct tccgaaacaa ccgagatcgg tccagccgag
    17941 atcgtcggca agaataaaaa caaaattctg ccggggtgct tccgggtggt gtgatactgt
    18001 ttcatggccg acacaggaag tcagcatggt actgaacgct gccgctttta aaaagtcccg
    18061 ccgtttcata caagcctctt ttctgctcct gagccctttg ccgttcggag cgcaaatgtc
    18121 tatagtacac accgccgcag aaaattgcaa cgaaatgtcg gatcatttcg gatcaatgac
    18181 gcatcggaga cggagatatt gcctgtggc
//
LOCUS       DAOPWB010000062        17603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3269_length_17603_cov_5.697287, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000062 DAOPWB010000000
VERSION     DAOPWB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17603
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3269_length_17603_cov_5.697287"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1164)
                     /locus_tag="PKN92_07325"
     CDS             complement(151..1164)
                     /locus_tag="PKN92_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ48641.1"
                     /translation="MAGLNYRRMLHAGTAGLKLLRGVLGLGRVPLFLSWNITFRCNLR
                     CAYCGASDAPRREMSAEEILAGTKEMYKLGARWITFGGGEPLLRSDIGEILHGASEQG
                     YQVFLSTNGSLLPKKIELLPAVDHINLSLDGPREIHDQVRGEGAFAGMMKGADAAAAA
                     GISYSFLCTLGAHNLNVVEETVRIARERQVWVMFQPATLWLDSSTADNPVAAQPEQYR
                     EAMEQLMALKAAGAPIANSRAGLRHLAQWPNHTKIRCLAGRLSVVIEPDGTMLSCHQY
                     EVGRFLEQTGGSGIRMGEQFRNLIPPRGCTQCWCAPVVELALIASFNPEAFWNSFRRV
                     FKE"
     gene            complement(1186..2640)
                     /locus_tag="PKN92_07330"
     CDS             complement(1186..2640)
                     /locus_tag="PKN92_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNZ48642.1"
                     /translation="MNTPSDKLAQLKQALGLKSGADISPGSASVRKKKSPGKAKARIS
                     ASDRGTEPPLSGSELSQRLYRHLGLGEHLSAVFPEKDSGEAVPLESLLGGELVENEEG
                     SFYLWKRSFPLTYQVGKVPLLEGLRCGGREIALSALDDSLSSFDPARACFLDAETSGL
                     AGGAGTVAFLIGVGYYSDNAFTLEQCFMRDYDDEPAMLRYLSEKLMPFETLVSYNGKS
                     FDAPLLRSRFVQNRVPFPLDRVPHYDLVHAVRRLWRRRLKDCSLGNIEASVLHFRREN
                     DVPGYLIPQLWFDYLEARDGRPLTGVFMHHAWDILSLAALAGHLANCLSEKEGSTLFH
                     AEDQVSLVRLYFRKKEYSQAVAQGEKFLEGSRINDLLRLDCLKLVGKAHKKLGNFEEM
                     RISLETIHREYPQDPEGAELLSQYYEHHSRNLIAARDVCRETLDCIAASPDAQRGLTD
                     FSHSSLQGRHRRLEKKLERLRGKHEDPDLPDMDE"
     gene            complement(2735..4507)
                     /locus_tag="PKN92_07335"
     CDS             complement(2735..4507)
                     /locus_tag="PKN92_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNZ48643.1"
                     /translation="MAKVTIDNEVVEVPDGTTILAAARRAGIKIPTLCYLEDVQAIGA
                     CRVCMVEVEGARTLVASCVAPVSDGMVVHTNSKKARAARKMVVELLLSDHTGDCQVCD
                     RNDDCELQALARDLGIRDITYQGEKSRRLVDDSTPALVRDSAKCVLCRRCVTVCADTQ
                     SVGGIFPQGRGFETVIGPAFAGELDDIVCVQCGQCAAVCPVGAISERDQVQEVWAALD
                     DPTKTVVVQTAPAIRAALGECFGLEPGTLVTGRMVTALRRLGFHAVFDTNFAADLTIM
                     EEGNELLTRLKKALTGEGDVALPMFTSCSPGWIKYIEYFYPDMLNNLSTCKSPQQMFG
                     AIAKTYYAQKIGKKPEDMIVVSIMPCTAKKYEAQRPEMRDSGVQDVDYVLTTRELGRM
                     ITEAGIDFVNLPDGKQDSPIGLGSGAADIFANTGGVMEAALRTAWEVVTGQELPFDNL
                     HVTPVAGLDGIKEASATFTNCLPEWSFLEGVTAKVAVAHGLGNARRLIEKIRAGEASY
                     HFIEIMTCPGGCIGGGGQPRMTTNAIRKKRIEAIYKEDEGKALRKSHKNPEILQVYEE
                     FLGAPLGELSHKLLHTKYTPRTKV"
     gene            complement(4529..7594)
                     /gene="nuoF"
                     /locus_tag="PKN92_07340"
     CDS             complement(4529..7594)
                     /gene="nuoF"
                     /locus_tag="PKN92_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNZ48644.1"
                     /translation="MKIARMHMVMCYGTGCVASGSPAVKAALLAALKKADLSDEVSVI
                     ESGCNGFCANGPILVVYPGGIFYHDLKPSDMEELVQEHVLKGRPVERLMFTDPVSNKP
                     VPLVKDIPFFSRQCVRVLSNKGRIAAESIEEYIAYDGYSAYLKALMRMKPEEIIAEVK
                     ASGLRGRGGAGFPTAIKWDLCRKSPGDLKYILCNADEGDPGAFMDRSILEADPHALLE
                     GMLIGALAIGASEGYIYCRAEYPLALERLRHAIAECRAMGLIGKNILGTDFSFDFHIA
                     QGSGAFVCGEETALMRSVEGKRGEPRPRPPFPAVAGLWEKPSVLNNVETFANIPLIIR
                     NGSDWYRSKGTEKSPGTKIFAVTGKINNIGLVEVPIGISLGEIVYDIGGGIPNGKAFK
                     AAQIGGPSGGCIPKEHLNVPIDFETLTELGAIMGSGGLIVMDEDTCMVDMARFFLDFV
                     QEESCGKCVPCRIGTKRMLEILDRICAGEGEEGDIEKLITLGEQIKDTSLCGLGQTAP
                     NPVLSTIRHFREEYEIHIREKRCPAGVCSGLVRAPCISACPAGVYVPGFVSLVGEKRY
                     VEALRVHRDKNPFASVCARVCFHACERKCRRADLDDPVAIRGVKRFMVDQEEAIQVPE
                     IRENAVNAKKKVAIIGAGPAGLTCGFFLARLGYKPTIFEAASKPGGMLVQTIPAYRLP
                     RKELEREIDMIQQMGVTVKTNQVLGSDFTLEGLRDEGYEAVFLGVGAPTGTRLSMPNA
                     DAEGVDDSLTFLSQYNLTGSAPVGKKVAVIGGGNAAIDAARTALRLGAESVTILYRRT
                     RDEMPAFKEEIDAAESEGVELKTLVLPQEIIAEDGKVVGVRCFNMWLGDFDSSGRRRP
                     QVSEDTEAFIVEADQVIAAIGQKLDSKPLLGSLTVNFAPNGFIAADPLTGQTSVPWLF
                     AGGDAATGPASVVEAVGAGERAAVGIDKFLSGEEHAFWRDEYAVDSDYDPDADPSMEG
                     RAQVEMLAAEERAHSFDEVELAFSESVALREAKRCLRCDYRAPGQTSCK"
     gene            complement(7608..8009)
                     /locus_tag="PKN92_07345"
     CDS             complement(7608..8009)
                     /locus_tag="PKN92_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNZ48645.1"
                     /translation="MSKDMNPITGIGDLQAIKERVLKQTALRNDGYRTCVTVHMGTCG
                     IAAGARDVMAAVMDELEKSNRTDIRVTTSGCIGICVQEPVMTIETLDGESALYGKLDD
                     EKARLAFREHALEGKVVTQLIIGKGREQITG"
     gene            complement(8006..8542)
                     /locus_tag="PKN92_07350"
     CDS             complement(8006..8542)
                     /locus_tag="PKN92_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNZ48646.1"
                     /translation="MVDKAQCCCSTEEATEEELLSRLDEVIAEYKGKPGALIPVLQMA
                     QAIFGYLPESALKKVALGLDKSYSEVAGVVSFYAFFSTNPKGRQTIRVCLGTACYVRG
                     GKQVLEALKSKLKIEVGEATPDRELSLEVARCFGACGLAPTMSIGEEVYKRVRPAKVG
                     EIIDEYYKNNVSVKGAAV"
     gene            complement(8596..9303)
                     /locus_tag="PKN92_07355"
     CDS             complement(8596..9303)
                     /locus_tag="PKN92_07355"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNZ48647.1"
                     /translation="MSLVRDISPRTIERLSLYRRLLLPRHEGEGSYIFSHDLAELAGV
                     TATQVRRDFMQLGCMGVPRTGYRIGDLLSGISNILDAPSGQRAALVGVGHLGAALLNH
                     FSGRWRWLHIHAAFDTDPAKTGGTIHGCQCFPLTDLSHLVRERNIELGIITVPKEHAQ
                     EVADLLITSGVRGLLNFAPLRLRHPATIYVENVDLTSAFETVAYFSRSRSSSALISEP
                     HRGFPSLDDRKLSEGCF"
     gene            9591..11675
                     /locus_tag="PKN92_07360"
     CDS             9591..11675
                     /locus_tag="PKN92_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNZ48648.1"
                     /translation="MLYNHGMNSFEKIPLVTTIKERCRVCYTCVRECPAKAIRVADAQ
                     ADILTARCIGCGNCVQVCGKHAKKAYDSTPELRTLMQGSDPLIALVAPSFPAAFPHIP
                     QERLVGILRAMGFSRVCEVSFGADLVARQYRKLLEGSPQAHHIATTCPAIVLYVEHFF
                     PDLVGALAPLASPMVAMARVVREYYDRNAKVVFIGPCIAKKEEARTSGNGQEIDVVLT
                     FEEMLRLASDMGIFVGRVKASPFDPPLGGLGGLFPLSRGLIQSAGLMEDLTSDDIITT
                     DGRHGFVEAIKEFQAGQCRPRLLEILACEGCIMGPGISNDDPLFRRRQRISAQVRQEM
                     ESLDRTQWEADLERFSSLDLSQTFAADDQRIFDPSRNDLEAILKQLGKFSPEDELNCG
                     ACGYGTCREHAVAIYKDLAEHEMCLPFVIDQLREAVRNLAQSKEELAQTQETLMHAEK
                     LASMGHLAAGIAHELNNPLGVILMYAHMLMEEHATDGPSVREDLEMVITQADRCKNIV
                     GGLLHFARQHKASFSFVSVHRFFEDLLKLMIIPENIEVRIVHESPAAEVYMDRDQIIQ
                     LMINLLTNACHAMPDGGVLTLRSQCGDHDNRFSVQDTGCGIPEEHVKRIFEPFFTTKD
                     VGQGTGMGLAVSYGIAKMHRGDLRVTSCTDAEAGSTGSTFTVILPRNPAGPTQADTRE
                     NPAQAAPVLHAD"
     gene            11716..12858
                     /locus_tag="PKN92_07365"
     CDS             11716..12858
                     /locus_tag="PKN92_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ48649.1"
                     /translation="MDTIKVLVVDDEIGIRVSIARILKKMTIVLSEEETEAHFEVEQA
                     ETGEQALQLIRESPPEILLLDNKLPGISGFQVLEQIAAMNLGIQTVMITAYASIENAI
                     HAARQGAYDMLPKPFTPVELKRVLEKVANHVIMARRARELAAEKKRVRFQFISVLAHE
                     LQVPINAIENYLRVMRDRTLGEDMKSYDAYLDRCLLRSEYMRKMISDLLDLTRIESGE
                     RARTLEPCNLVEAARLAMDALAGEAAQRGIELVLDTRRVSILADHTEIDIILNNLISN
                     AIKYNRDQGKVEIAFYKKGDEVKIQVADTGIGMSAEDCSRIFNDFARIKNTRTSRILG
                     SGLGLSTVKKIALLYNGSVSVSSVPDEGTTFTVILKDAGAADSTNR"
     gene            complement(12974..17425)
                     /locus_tag="PKN92_07370"
     CDS             complement(12974..17425)
                     /locus_tag="PKN92_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48650.1"
                     /translation="MLKLVSVSNQEPWTASFGYYQIRYKATDSGGKTAQVTVMAIPRS
                     CETGGEIIGCHPFIEKPILAVQQNMEYCTRASGSNHFLYTLDCWDAWEFFGPVTAWGL
                     DICRNKLDVVTHVEFWQDNGDAGAWEPIETLGLNMNTWSSRPGSYRVFYMVTNGDPKG
                     VWVSRYVIADVADNCTYYSGGAGTEGDPYLIANVRDWRRLMLSESDWDKHFRVIADID
                     LTQVHVFPVGNADVPFTGVMDGQGHTLSNCAVENVGGFQNNVGLFGAVGGGAYIHNLH
                     LENASVPGNQGGGGLAGSVTESRLENCSVSGIVKGTTFIGALVGHAINSEITSCSADA
                     EVEASGHAGVLIGVAEDCAVSFCSSAGLLVSYTSAGGLVGRQVGGIISDSTSAAEVQG
                     FEDTGGFVGTMQGGEISRSTATGKVVGTYANTGGFVGHMNQEAMIFLSSASGQVEGAL
                     AAGGFAGKLYQATIRQSSAVGSVTGEQRVGGLVGQLLEGFVRNSYARGSASAPLRYAG
                     GLIGTLNYGTVKNCYSTGVAESNILPGGLIGYYINGFVQQSYWDTEASGLAASGAGEG
                     RTTEEMTAPHAENTYLDWDFGEIWIEDTDGTVNDGYPYLHTGVVDIDDCDPDITPPEI
                     KFEHRPENSQWLEDIGFYVYTLNCGDDWNVRGPISVEAVDNCAGYFLLHSEILGLIED
                     EEENLEPVPLEDLGVDINSWTGRPGLYVVNYSATDISGNSTEVHVLVLIEDNCYVPEG
                     EPVEGEIEGEDECDPDEAAPEVVLSHRSDGKWDAVRGMVVYKIDCSDSWSEKGPSKAS
                     AVDNCDVDLVPVMTIAALIGGLEVPLEEAGLDPGSWTRAFGEYVITYTATDQAGNTAF
                     EKVGALVADRSAPEIHIVQADANVAWDNAAKRFVYTLEVGSDWEVYGPKSVTADDKCE
                     GALEVTRQIFVLVPGQNGEKIITLAEAGLDEENWSRTLGTYLVRYSAEDDSGNIDTES
                     VAVKLRDTTAPVVVVTNGENGSWSDDEVPVFVYSLDCQDDWAAKGPIDVTATDNYDGD
                     LPVTTVIRKLRLRNGVYIEETLESQGLNENNWTGHPGLYLIRYTAVDSSGNSSTESIR
                     ALVEDRSAPVITIVHPTAGGGEEPYVYTLDCGDSWETHGPVSVTATDLCDGDRPVVSV
                     ITTADGTGVRDGWTKIPGNYTAKYTATDKAGKTATETVQIVISDKTAPVVTIAHRQGS
                     SYSADENLYTLNCGDLWINIGPVSASAWDQCDGVISKVTSVITALVPAGEGYEEVSLE
                     SLGLNNTTWVRTPGLYLVRYTAKDAAGNVGTAVTRALVDDRILPVFTATHREGNGVWL
                     EAGLFQYTLECGADWRSQGPSRVVASDNCDGSLPVSSVVEALVDGETVPVADPNSWTS
                     VPGLYVVRYTATDRSGNKAELLVQALIADCDEVEGEEEDLSYHPFDGNNNNRINGFEA
                     SDSIRAWQNGDFPMEWALRALYIYKQGGAYHYDDAFDPPLCWVPDKS"
BASE COUNT         4043 a         5246 c         4400 g         3914 t
ORIGIN      
        1 gttcccattc ctgcagaaat gccgttgccg aagcatcact ttccgccatc catttttgta
       61 agagcgccca ctcccctttt tctttctgtg ccagacgtct gggtatcatt ttggtttcct
      121 gttctataga cgaaattcat ttgtgtattg ttattcttta aaaacacggc ggaaactgtt
      181 ccagaacgcc tccggattga acgaggcgat cagagcaagc tctaccaccg gcgcgcacca
      241 gcattgtgta cagccacggg gcgggatcag attccggaac tgctctccca tcctgatacc
      301 cgatccaccc gtctgctcaa gaaaacgacc cacttcatac tggtgacagg aaagcatggt
      361 accgtcgggc tcaatcacca cagagaggcg tccggcaaga cagcggatct tggtatgatt
      421 cggccactgt gccagatggc gcagtcccgc ccgggaattg gcgatgggtg cgcctgccgc
      481 tttcaacgcc atcagctgct ccattgcttc tctgtactgc tccggctgcg ctgcgacagg
      541 gttgtcggcg gtggaggaat cgagccaaag ggttgcaggc tgaaacatga cccagacctg
      601 ccgctcccgg gcaatccgta ccgtctcctc cacaacgttc agattgtgcg cacccagtgt
      661 acataaaaaa gaataggaga tacccgctgc cgccgccgca tctgcccctt tcatcatccc
      721 ggcaaacgcg ccttccccgc gcacctgatc atgaatctcc cggggtccgt ccagactgag
      781 gttaatatga tctaccgcag ggagcaactc tattttttta ggaaggaggg aaccattggt
      841 gctgaggaac acctggtaac cctgctccga tgcgccgtgc agaatctctc cgatatcaga
      901 tctgagcagc ggttctccgc ccccgaacgt gatccagcgc gcaccgagct tgtacatttc
      961 cttcgtgccg gcaagaattt cctcagccga catctcacgt cggggcgcgt ccgaagcacc
     1021 gcagtaggca cagcgcaggt tacaccggaa tgtaatattc caggaaagaa aaagcggcac
     1081 cctgcccaga ccgaggacgc cgcgcagcag tttcaggccg gccgttccgg cgtgcaacat
     1141 gcggcgatag ttcaatccgg ccattgagtg gttctcctgt tccgcttact catccatatc
     1201 cggcaaatca ggatcttcgt gtttcccgcg cagacgctcc agtttctttt ccagccgccg
     1261 atgccttccc tgaagcgaag agtgggaaaa atcagtcagg ccacgctgcg catcaggcga
     1321 ggcggcaata caatcgagtg tttcccggca gacatccctg gcggcaataa gattgcggct
     1381 gtgatgttca taatactggg aaagcagttc agcaccttca ggatcctgcg ggtattcccg
     1441 atgaatcgtc tcaaggctga tccgcatctc ttcaaagttg cccagttttt tatgagcctt
     1501 tcccacgagc ttcagacaat ccaggcgcag caggtcgttt atgcgggagc cttccagaaa
     1561 tttttcaccc tgtgccactg cctgactgta ttcttttttt ctgaaataaa gacgtaccag
     1621 cgacacctga tcttccgcat gaaaaagcgt gctgccctct ttttccgaaa ggcagtttgc
     1681 cagatgcccc gccagtgccg caagcgaaag gatatcccag gcgtggtgca tgaacacgcc
     1741 ggtcagcggt ctgccgtccc gcgcttcaag ataatcaaac cagagttgag gaatcagata
     1801 gccgggcaca tcattttcac ggcggaaatg aagcacggag gcttcaatat ttccgaggct
     1861 gcaatctttc agacgcctac gccacagacg gcgtaccgca tgtaccagat cataatgagg
     1921 cacccgatcg agaggaaaag gaacccgatt ctggacaaag cgcgagcgga gcagaggcgc
     1981 atcaaaactt ttcccgttgt aactgaccag cgtttcaaag ggcatcaatt tttccgacag
     2041 gtagcggagc atggcaggtt catcgtcgta atcccgcata aaacactgct ccagagtaaa
     2101 ggcgttgtcg ctgtaataac ccacgccaat cagaaaggcg accgtacccg ctccacccgc
     2161 cagaccgctt gtttccgcat cgagaaaaca cgcccgcgcc ggatcgaaag aagaaaggct
     2221 gtcatcaagg gcgctgagcg ctatctcccg accaccgcag cgaagccctt cgagaagagg
     2281 cacctttcct acctgatacg ttaagggaaa agaacgtttc cacagataaa aggaaccttc
     2341 ttcattttcc acaagctctc cgcccagcag agattccagc ggcaccgctt cccccgagtc
     2401 cttttcagga aaaacagcgg agaggtgctc ccccagcccc agatgacggt acagccgctg
     2461 tgacagttcc gatccggaca gaggcggttc tgttcctcta tctgaagcgg atatccgtgc
     2521 cttcgctttg ccgggtgatt tctttttccg aaccgacgca ctccccggcg agatatccgc
     2581 accagatttc agaccgagcg cctgcttgag ttgtgccagt ttatccgaag gtgtattcat
     2641 gctcttacta taccgcaatg acgaagaaaa aaaacagcag ccggcgcgaa aacacaccgg
     2701 ctgctgcagc agtcatccag tcaggtcaga agtctcagac cttggtacgg ggcgtatatt
     2761 tggtgtgaag cagtttatga gacagctcac ccagcggtgc gcccagaaat tcctcataaa
     2821 cctgaaggat ttcagggttc ttatgggact tgcgcagtgc cttgccttcg tcttccttgt
     2881 aaatggcttc gatgcgtttt ttgcggatgg cattggtggt catacggggc tgcccgccac
     2941 cgccgataca gccgccggga caggtcatga tttcgatgaa gtgataggat gcttcaccgg
     3001 cacggatctt ttcaatcagt ctccgggcgt tgccgaggcc gtgggcgacc gccacttttg
     3061 cggtaacgcc ttccaggaaa gaccattcag gcagacagtt ggtaaaggtg gcggaggctt
     3121 ccttgatccc gtcgagacct gcaaccgggg tcacgtgcag attatcgaag ggcagttcct
     3181 gtccggtcac cacttcccaa gcagtccgca gtgcggcttc catgacaccg ccggtattgg
     3241 caaaaatgtc cgcagcaccg gaacccagcc cgatgggaga atcctgcttc ccgtcgggaa
     3301 ggttcacgaa gtcaataccc gcttccgtaa tcatacggcc cagctcacgg gttgtcagca
     3361 catagtccac atcctgcaca ccgctgtcgc gcatctcggg ccgctgcgcc tcgtacttct
     3421 tggcggtgca gggcataatc gacacgacaa tcatatcctc gggcttcttg ccgatcttct
     3481 gggcatagta ggtcttggca atggcaccga acatctgctg cggcgatttg caggtggaca
     3541 gattgttaag catgtccgga tagaagtact caatgtactt gatccagccc ggagagcagc
     3601 tcgtgaacat gggaagcgcc acgtcgcctt caccggtcag tgcttttttc agccgggtca
     3661 gcagctcgtt accctcttcc ataatcgtca ggtcggcggc aaagttggtg tcgaaaaccg
     3721 catggaatcc gaggcggcgc agtgccgtca ccatgcgtcc ggtcaccaag gtgcccggct
     3781 caaggccaaa gcattcgccc agtgctgcac ggatcgccgg agccgtctga acgaccacgg
     3841 ttttcgtcgg atcgtccagc gccgcccaga cttcctgcac ctgatcccgc tcggaaatcg
     3901 caccgacagg gcataccgcg gcacactgcc cgcactggac acagacgata tcatcgagtt
     3961 cgccggcaaa ggcgggcccg atcacggttt caaaaccgcg gccctgcggg aaaataccgc
     4021 ccacactctg ggtgtcggcg cacacggtaa cgcaacggcg gcacagcacg cactttgcgc
     4081 tgtcgcgcac cagtgccggg gtgctgtcgt ccacaagtct gcggctcttt tcaccctgat
     4141 aggtgatgtc ccggatgccg agatcccgtg cgagcgcctg cagttcgcag tcgtcgttgc
     4201 ggtcgcacac ctggcagtcg ccggtatgat cggagagcag cagctccacc accattttac
     4261 gggcggcacg cgcttttttg ctgttcgtat gcacgaccat gccgtcactc accggcgcaa
     4321 cacaagacgc gaccagcgta cgggcgcctt ccacttccac catacataca cggcaggcac
     4381 caatggcctg aacgtcttcc aaataacaca gggtggggat tttaatgccc gcccgccgcg
     4441 cagcggcaag aatggtggtg ccatcaggca cttccaccac ttcgttatca atggttactt
     4501 ttgccattat ttcaatctcc ttgatgggtt atttacacga agtctgaccg ggagcacggt
     4561 aatcacagcg caggcagcgt ttggcttcac gaagcgccac ggattcactg aaggccagtt
     4621 ccacttcatc gaaactgtga gcacgttctt ctgcagccag catttcgacc tgcgcacggc
     4681 cctccatgga agggtcagcg tccggatcgt aatccgaatc cacggcatat tcgtcacgcc
     4741 agaaggcatg ctcttctccg ctcaggaatt tgtcaatgcc tacggcggca cgttctccgg
     4801 caccgaccgc ttcgacgacc gacgccgggc cggtagcagc atcaccgccc gcaaagagcc
     4861 agggcaccga tgtctgtccg gtcagcggat cagctgcgat aaagccgttg ggcgcaaaat
     4921 tcacggtcag cgatccgagc agcggcttgc tgtcgagctt ctgaccgatg gcggcaatga
     4981 cctgatcggc ttccactata aaggcttccg tatcttcact gacctgaggc ctgcggcggc
     5041 cgctgctgtc gaaatcgccg agccacatgt tgaagcaccg cacaccgaca accttgccgt
     5101 cttccgcaat aatttcctga ggcagtacca gcgttttcag ttcaacgcct tcgctttccg
     5161 ccgcatcaat ctcttccttg aaggcaggca tttcgtcccg ggtgcgccgg tacagaatgg
     5221 tgaccgattc agcgcccaga cgcagcgctg tacgcgccgc atcaatggct gcgttaccgc
     5281 cgccgatgac cgccactttc ttgcctacag gtgcgctgcc ggtaagattg tactggctga
     5341 ggaaggtgag ggaatcgtcc acgccttccg catccgcatt gggcatgctc agccgggtgc
     5401 cggtgggagc gccgacgccc agaaacactg cctcgtatcc ttcatcccgc agtccttcaa
     5461 gggtaaagtc tgagcccaga acctgattgg tcttcaccgt gacacccatc tgctgaatca
     5521 tgtcaatttc acgttccagc tcttttctcg gcaggcggta ggcgggaatt gtctgcacca
     5581 gcatgccgcc cggtttggac gccgcttcaa aaatcgtcgg tttgtaaccc agccgcgcca
     5641 ggaagaaacc acaagtcaga ccggcaggac cggcaccgat aatggcaact ttctttttcg
     5701 cattgacggc attttcacgg atttcaggaa cctgaatggc ttcttcctga tccaccatga
     5761 accgcttcac gccacggatt gcaacgggat catccagatc ggcgcgacgg catttccgtt
     5821 cacaggcatg gaaacataca cgtgcgcaga cagaggcaaa ggggttcttg tcacggtgca
     5881 cacgaagcgc ttcaacataa cgtttttcgc ccacaagcga cacgaacccg gggacataca
     5941 cgcctgccgg gcaggcgctg atacaagggg cacgaaccag gcccgaacag acgccggccg
     6001 gacacctttt ctcgcgaata tggatttcat attcttcgcg gaagtggcgg attgtggaga
     6061 gcaccggatt gggagccgtc tggccgagtc cgcacaggga agtgtcttta atctgctctc
     6121 cgagggtgat cagtttttca atatcgccct cctcgccttc tccggcgcag atcctgtcga
     6181 ggatctcaag catacgcttg gtgccgatac ggcaggggac gcatttgcca caggactctt
     6241 cctgtacgaa atccaggaaa aatcgcgcca tgtccaccat gcacgtgtct tcatccatta
     6301 caatcagacc gccggagccc ataatggcac ccagctctgt cagcgtctcg aaatcaatgg
     6361 gcacattcaa atgctcttta ggaatgcagc cgccggaagg cccgccaatc tgcgctgctt
     6421 tgaatgcttt gccattggga ataccaccgc cgatatcgta aacgatctcg ccgagagaaa
     6481 tgccgatggg tacttccacc agcccgatat tgttgatctt gccggtcaca gcaaaaatct
     6541 tcgtgcccgg gcttttttcc gtacccttgc tgcgatacca gtcactgccg ttgcggataa
     6601 tcagcggaat attcgcaaat gtttcgacgt tgttcagcac agatggtttt tcccacaggc
     6661 cagccacagc cgggaaaggt ggacgcgggc ggggttcgcc ccgcttgcct tccaccgaac
     6721 gcatcaacgc cgtctcttca ccgcatacaa aagcacccga tccctgggca atatggaaat
     6781 caaaggaaaa gtccgtgccc agaatgtttt tgccgataag tcccatggca cggcattccg
     6841 caatggcgtg gcgcaggcgc tccagcgcca gagggtattc tgcacggcaa tagatgtagc
     6901 cttccgaagc gccgattgcc aacgctccga tcagcatgcc ttccagcagc gcatgaggat
     6961 ccgcttccag aatactgcgg tccataaacg ctcccgggtc accttcatcc gcattgcaga
     7021 gaatgtattt caaatcgcca ggcgattttc tgcacagatc ccacttgatg gctgttggga
     7081 aacccgcacc gccgcgaccg cgcaggcccg atgccttcac ttccgcgatg atctcttcag
     7141 gtttcatccg catcagcgct ttgagatagg cgctgtagcc gtcataagcg atgtattcct
     7201 caatgctttc cgccgcaata cgccccttgt tcgacagaac acggacacac tgacgggaga
     7261 agaagggaat atccttcacg agcggaacgg gcttattgct caccggatca gtgaacatca
     7321 gccgttccac cgggcgcccc ttcagcacat gctcctgcac cagctcttcc atgtcggaag
     7381 gcttcaggtc gtgatagaaa atgccgccgg gatacacaac aagaatgggg ccgtttgcgc
     7441 agaaaccatt gcatccggac tcaatcacag atacttcgtc agaaaggtcg gcttttttga
     7501 gtgcagcaag aagcgccgct tttacagcag gcgagccgct ggcaacacag ccggtgccat
     7561 agcacataac catatgcatt cgggcaatct tcatagagcc agtctcctta acccgttatt
     7621 tgttctctgc ctttaccaat gatcagctgg gtgacgacct tgccttccag cgcatgctcg
     7681 cgaaaagcga gacgtgcttt ctcgtcgtcc agttttccgt acagtgcgct ctctccgtcc
     7741 agcgtctcaa tagtcatcac aggctcctgt acgcagatgc ctatgcagcc ggaagtagtg
     7801 acccggatgt ccgtgcggtt gctcttttcc agctcgtcca tgacagcagc catcacatca
     7861 cgggcacccg ccgcaatacc gcaggttccc atatgcaccg tgacacaggt acggtagccg
     7921 tcgttgcgga gtgccgtctg tttcaacacg cgctctttga ttgcctgaag atctccaatt
     7981 ccggtaatcg ggttcatatc tttgctcata ctgctgctcc tttcacagac acgttgttct
     8041 tgtaatactc gtcaataatt tcaccgactt tggcaggacg cacacgtttg tagacttctt
     8101 ctccaatgga catggtggga gccagaccgc aggcaccgaa acagcgtgcc acttccagag
     8161 aaagttcccg gtccggtgtc gcttcgccca cttcgatttt cagtttgctt ttcagtgctt
     8221 ccagaacctg cttgccgcca cggacataac aggctgtacc caggcacaca cggatggtct
     8281 gccgtccttt gggattggtc gagaaaaagg cgtaaaaact caccacgccc gccacttcgc
     8341 tgtaagactt gtccagaccc agagcaacct ttttcagtgc cgattcaggc aaatagccga
     8401 aaatcgcctg ggccatctgc agcaccggaa tcagcgcgcc gggcttacct ttgtattctg
     8461 caatcacttc gtcaaggcgg ctgagcaatt cctcctccgt cgcctcttcc gtgctgcagc
     8521 aacactgcgc tttgtctacc atcatagctc tccttgcatg ctgaccggaa tcccttcgga
     8581 tgccggagtt aaaaattaaa aacagccttc cgaaagtttc cggtcgtcca atgaggggaa
     8641 accgcggtga ggctcactta tcaacgcaga cgatgaacgg gaacgcgaaa aatacgccac
     8701 cgtctcaaaa gccgaagtca gatccacatt ttcaacataa atggttgcgg gatgccgaag
     8761 gcggagaggg gcaaaattta aaagaccgcg cacacccgaa gtaatcagaa gatcagccac
     8821 ctcctgcgca tgctccttag gcacggtaat aatgcccagt tcgatattcc gctcgcgaac
     8881 cagatggctc aggtcggtca gcggaaaaca ttgacagccg tggatagttc cccctgtttt
     8941 tgctggatcc gtatcaaaag cggcatgaat gtgcagccag cgccagcgtc cggaaaaatg
     9001 atttaaaagt gccgccccca gatgccccac gcccacaagg gccgcccgct gccccgacgg
     9061 cgcgtcaaga atattgctga tcccgctcaa taaatccccg atacggtacc ctgtgcgcgg
     9121 tacacccatg caccccagct gcatgaaatc ccgccggacc tgtgtcgctg taacccccgc
     9181 caactccgca agatcatgag aaaagatata actgccttct ccttcgtgcc tcggaaggag
     9241 cagcctgcgg tacaggctga gccgttctat cgttctaggc gatatatcac gaaccagaga
     9301 catcgtttac cgcactccag aaacgggagt cactcccgtc tgttgtcgtc ctgaaccttc
     9361 gatcagttca gcggcataaa caaaagtttt gtctgctata cctaccgttc ggtatataaa
     9421 gacacaatca aaatatagtc gtgaaagaaa taacgattct catactataa cataacaata
     9481 catacattgc aacactttta gcagaaactt tttccgtcga aaacgtacaa ctgcagtacc
     9541 aaaataattg tgcagcgttt cacaaaaaat gccttgcctc atgccgcccc atgttgtaca
     9601 atcatggcat gaacagcttt gaaaaaatac cgcttgtcac aaccatcaag gaaaggtgcc
     9661 gcgtctgtta tacctgcgtg cgggaatgcc ctgccaaagc aatccgggtc gcagacgcac
     9721 aggcggatat ccttacggca cgctgcatcg gctgtggcaa ttgtgttcag gtgtgcggca
     9781 agcacgccaa gaaagcctac gattcaacac cggaactccg cacgctgatg cagggctccg
     9841 atccgctgat tgcgcttgtt gccccatcct tccccgccgc ttttccgcat attccgcagg
     9901 agcgccttgt gggcattctg cgcgccatgg gtttttcgcg ggtctgcgaa gtatccttcg
     9961 gcgccgatct ggtcgcccgc caataccgca aactgcttga aggctcgccc caggcccatc
    10021 atattgccac cacctgccct gccattgttc tttatgtgga gcattttttt cccgatctcg
    10081 tcggagccct cgccccgctt gcctcaccga tggttgccat ggcacgtgtt gtccgggagt
    10141 attacgaccg taacgccaaa gtggtcttta tcggtccgtg tattgccaag aaagaagagg
    10201 cgcgaacctc cggcaacggt caggaaattg acgtcgtcct cacctttgaa gagatgctgc
    10261 gacttgcttc tgatatggga atatttgtcg ggcgggttaa agcatcgccc tttgatccgc
    10321 ctctgggtgg cctgggcgga ctcttccctc tcagccgcgg tctcattcag tccgccggac
    10381 tcatggaaga tctgacctca gacgacatta tcacaacaga cgggcgccat ggttttgttg
    10441 aggcaatcaa agagtttcag gcgggccaat gccgtccgcg tctcctcgaa atactggcct
    10501 gtgaaggctg tattatgggt ccgggcattt ccaacgacga tccgcttttc cggcggcgcc
    10561 agcggatcag cgctcaggta cgccaggaaa tggaatcgct cgaccggaca cagtgggaag
    10621 cggatctcga acgtttttcc tctctcgatc tgagccagac ttttgccgcc gacgaccagc
    10681 ggatctttga cccttcccgg aatgatctgg aagccatact caagcagttg ggcaaatttt
    10741 cgcctgagga tgagctgaac tgcggcgcct gcggttacgg cacctgtcgc gaacatgccg
    10801 tggcaatcta taaagatctg gctgaacatg aaatgtgcct tccctttgtg atagatcagt
    10861 tgcgggaagc ggtcagaaac ctggctcagt ccaaggaaga acttgcccag acccaggaaa
    10921 cgcttatgca tgcggaaaaa ctcgccagca tggggcatct tgccgccgga atcgcccatg
    10981 agctcaacaa ccccctcggc gtgatcctca tgtatgcaca catgcttatg gaggaacatg
    11041 caacggatgg tccctctgtc cgggaagatc tggagatggt tatcacccag gcagaccggt
    11101 gcaaaaatat tgtgggcggc ctgctgcatt ttgcacggca gcacaaggcc tctttttcct
    11161 ttgtcagtgt gcatcgtttt tttgaagatc tgctgaagct gatgatcatt ccggaaaata
    11221 tcgaggtacg cattgtccat gaaagtcccg ctgctgaggt gtatatggat cgggatcaga
    11281 tcatccagct catgattaac ctgttaacca atgcctgcca tgccatgcct gacggcggcg
    11341 tgctcactct cagatcacaa tgcggtgatc acgacaaccg cttctccgtg caggataccg
    11401 gatgtggcat ccctgaagag catgtaaaac gcattttcga gcccttcttc acaacaaaag
    11461 acgtcgggca gggcacaggc atgggactcg ccgtatcgta cggcattgcc aaaatgcacc
    11521 gtggtgacct gcgcgtcaca agttgcacag atgcggaagc aggaagcaca ggaagtacct
    11581 ttaccgtgat tctgccacgc aatcccgccg ggcccactca ggcggacacc cgggaaaatc
    11641 cggcacaggc agcaccggtt ctgcacgctg attaaaataa accgaaaggc gcattagaaa
    11701 gaaaggcgga agcccatgga taccatcaaa gtactcgtgg tggatgatga aatcggcatc
    11761 cgcgtgagta ttgcccgcat ccttaaaaag atgacgattg tcctctccga agaggaaaca
    11821 gaagcgcatt ttgaagtgga gcaggcagaa acgggggaac aggcactcca gctcatacgg
    11881 gaatcacctc cggaaattct gctgctggac aacaaattgc cgggcatcag cggttttcag
    11941 gtgctcgaac agattgccgc catgaatctg ggcattcaga ccgtcatgat cacggcgtat
    12001 gcctccatcg aaaacgccat tcatgccgcc cgccagggtg cctatgacat gctcccgaag
    12061 cccttcacgc ctgtggaact gaagcgggtc ctcgaaaaag tcgccaatca tgtcatcatg
    12121 gcacggcgtg cccgggagct tgccgcagaa aagaaacgtg tgcgtttcca attcatttcc
    12181 gtactggcgc atgaactgca ggtgcctata aacgccatcg aaaactatct gcgtgttatg
    12241 cgcgaccgga cgctgggcga ggatatgaaa agctatgacg cctatctgga ccgctgcctt
    12301 ttacgttcgg aatatatgcg caaaatgatc tccgatctgc ttgacctcac ccgcatcgaa
    12361 tcgggagaac gggcgcgcac cctcgaaccc tgcaatctgg tcgaagcggc gcggctggca
    12421 atggatgcgc tggcgggtga ggcggcgcaa cggggtatcg aactggtatt ggacacacgg
    12481 cgtgtatcca tactggctga tcatacagaa attgacatca ttctgaacaa tctcatttcc
    12541 aatgccatta aatacaaccg ggatcagggg aaagtggaga ttgcctttta taaaaaaggc
    12601 gatgaagtga aaattcaggt agccgatacc ggcatcggca tgagtgcaga agactgctcc
    12661 cgtattttta acgacttcgc aagaattaaa aatacgcgaa ccagcagaat cctcggcagc
    12721 ggactgggac tgtccacggt gaagaaaatc gcactcctct ataacggttc ggtctctgta
    12781 agcagtgtcc cggatgaagg cacaaccttc accgtcattt taaaagatgc cggtgctgcc
    12841 gacagcacta accgctaatc ttttctcccc cctctttcca tcccggacgc cgaaaaggaa
    12901 accgcccgat gcagccgctt ttcagcggat acaccgggcg gcgcagcctg tcagcgtaca
    12961 gcctgatcag gcatcagctc ttgtcaggca cccagcacag cggcgggtca aaggcatcat
    13021 cataatgata agcaccaccc tgcttgtaaa tgtaaagcgc ccgaagcgcc cattccatgg
    13081 ggaaatcgcc gttctgccag gcacgtatgc tgtccgaagc ttcgaaacca ttgatccggt
    13141 tattattatt tccatcaaag ggatggtagc tcagatcctc ttcttcgcct tcgacttcgt
    13201 cacagtctgc aataagcgcc tgaaccagca gctcggcttt gtttccggat cggtctgttg
    13261 ccgtgtagcg aacaacataa agtccgggaa cactcgtcca gctgttcggg tcggcaacag
    13321 gaaccgtctc tccgtcaacc agtgcttcca ccacagagga caccgggaga cttccgtcgc
    13381 agttatccga agcgacaaca cgggacggac cctgagaccg ccagtcagca ccacactcaa
    13441 gggtgtattg gaacaaccct gcctcaagcc acacaccgtt gccttcacgg tgtgttgccg
    13501 tgaaaactgg aaggatcctg tcgtcaacaa gcgcacgggt aaccgctgtg cccacattgc
    13561 ccgccgcatc tttcgccgta taacggacaa ggtacagtcc gggggtgcgc acccaggtag
    13621 tgttgttgag tccaaggctt tccagactca cttcctcgta cccctcgccg gcaggcacca
    13681 gtgccgtaat caccgatgtc actttgctaa tgacaccgtc acactgatcc cacgccgatg
    13741 cgctcacagg accgatgttt atccagaggt ctccgcagtt aagtgtgtac agattttcat
    13801 ctgccgagta gctgctgccc tgacgatgcg ctatcgtcac tacaggtgct gttttgtcgg
    13861 aaatcacgat ctgcaccgtt tccgtggcag tttttcctgc cttgtctgtc gcggtgtact
    13921 ttgctgtgta atttccggga atctttgtcc agccgtcacg cacgccggtt ccgtccgctg
    13981 ttgtgatcac ggaaacaacc ggtctgtcac cgtcacaaag gtctgtggca gttacagata
    14041 cggggccatg cgtttcccag ctgtctccgc agtccagcgt atacacatag ggttcttcac
    14101 cgccgcccgc tgtcgggtgc acaatggtaa tcacaggagc cgagcggtct tccacaagag
    14161 cccggattga ttccgtggag gaattgcccg agctgtccac tgccgtatac cggatcagat
    14221 aaagaccggg atgtcctgtc cagttgtttt cattcagacc ctgcgactcc agggtttctt
    14281 caatatacac accgttccgc agacgcagtt ttctaattac tgtcgtcacg ggaagatcgc
    14341 cgtcatagtt gtcagttgcc gtaacgtcaa tcggtccttt cgctgcccag tcgtcctgac
    14401 aatccagact gtaaacaaaa accggcacct catcatccga ccagctgccg ttttcgccgt
    14461 ttgtcaccac aaccaccggg gctgtcgtat cccggagctt caccgccaca gattccgtat
    14521 cgatattgcc ggagtcgtct tcggcagaat aacggacaag ataggtaccc agcgtgcgac
    14581 tccagttttc ttcgtccagt cccgcctctg ccaaggtaat gattttctct ccgttttgtc
    14641 cgggaaccag aacaaagatt tgccgggtaa cttccagagc accttcgcat ttatcgtcag
    14701 ccgtcacgga tttgggcccg taaacctccc agtcactgcc cacttccagt gtgtagacaa
    14761 aacgcttggc cgcattgtcc caggcaacat tcgcatccgc ctgcacgatg tgaatctcgg
    14821 gtgcggatct gtctgccacc agggcaccga ctttttcaaa cgccgtattt cctgcctgat
    14881 ctgtcgctgt atatgtaatc acatattcgc cgaaggcacg tgtccagctg cccggatcca
    14941 gaccggcttc ttccagagga acctcgaggc caccgataag agctgctata gtcattaccg
    15001 gcaccagatc cacgtcgcag ttgtcaacag cactcgcttt gctggggcct ttttcgctcc
    15061 aggaatcgct gcagtcgatc ttatagacaa ccatgccccg cacggcatcc catttcccgt
    15121 cactgcggtg gctgagcaca acctcaggcg ccgcttcatc gggatcgcat tcgtcttcgc
    15181 cttctatttc gccttcgact ggctcccctt caggcacata gcagttgtct tcaatgagca
    15241 ccagaacgtg cacttccgta ctgttgcccg aaatgtcggt cgccgagtag ttaaccacat
    15301 acaaaccagg cctgcctgtc caggagttga tatccacgcc cagatcttcc agtggcaccg
    15361 gctccagatt ttcttcttcg tcttcaatca gccccagaat ttctgagtgc agcaaaaaat
    15421 aacctgcgca gttatcgact gcttccacag aaatgggtcc tcttacgttc cagtcatcac
    15481 cgcaattcaa cgtatagaca tagaaaccga tatcttccag ccactgcgaa ttctccggac
    15541 gatgctcaaa cttgatttca ggaggtgtga tgtccgggtc acaatcatca atgtccacta
    15601 cgcctgtatg caaataggga tacccgtcat tgacggtgcc gtccgtatct tcaatccaga
    15661 tttctccaaa atcccagtcc agatacgtgt tttcagcatg gggcgcggtc atctcctcag
    15721 tcgtgcgccc ttcgcccgca ccgctcgcag ccaggccgga ggcttcagtg tcccaatagg
    15781 actgctgcac aaagccgttt atgtaatagc cgatcaatcc gcccggcaga atattactct
    15841 cggcaacgcc ggtggagtaa caatttttta cagtaccgta gttgagcgtt ccgatcaatc
    15901 cgcccgcata gcgcagaggc gcacttgctg aacctctcgc ataggagttt cggacaaaac
    15961 cttcaagcaa ttgaccgacc agtccgccca cacgctgctc acctgttact gatccgaccg
    16021 ctgaggactg gcggatagtt gcctgataga gtttacccgc aaatccaccg gcggccaggg
    16081 caccctcaac ctgaccgctg gcagaagaga gaaagatcat tgcttcctgg ttcatgtgtc
    16141 ccacaaatcc gcctgtattc gcataagtgc ccacgacttt ccctgtcgca gtgctccgac
    16201 tgatctctcc gccctgcatt gtcccgacaa aaccgcccgt atcctcgaaa ccttgcactt
    16261 cagcagcaga ggtgctgtct gaaataatac cgccaacctg tctgccgacc aggccgccgg
    16321 cactcgtata gctgaccagc aagccggcag aggagcagaa ggatacggcg cagtcctcag
    16381 caacacctat gagcaccccc gcatgccctg atgcttccac ttcggcgtcg gcggaacacg
    16441 aagtaatctc actgtttatc gcatgcccga ctaaagcgcc tataaaggta gtgcccttta
    16501 ctatgccgga tacggaacaa ttttccagac gggactcagt cactgatccc gctaaaccgc
    16561 ccccaccctg atttcccggc acactggcat tctccagatg cagattgtga atataggccc
    16621 ctcctcctac ggcgccgaaa agtccgacat tattctgaaa gccgccgaca ttctccactg
    16681 cgcaattgga aagcgtatgc ccctgaccat ccataacgcc ggtaaagggc acatctgcgt
    16741 tgcctacggg aaacacatgg acttgggtta aatctatatc tgctatcacc cggaaatgct
    16801 tatcccagtc gctttcgctc agcatgagcc ggcgccagtc ccgcacgtta gcgataagat
    16861 aaggatctcc ttcagtaccc gctccgccgg aatagtaggt gcaattgtct gctacatcgg
    16921 caataacata gcgcgacacc cagacacctt tcgggtcacc attcgtaacc atgtaaaaca
    16981 cccggtacga accgggccga ctgctccagg tgttcatatt aaggcccaga gtttcgatag
    17041 gctcccaggc tccggcatcg ccgttatcct gccaaaattc aacatgcgta acaacatcga
    17101 gtttgttgcg gcatatatcc agtccccagg cagttacagg accgaaaaat tcccaggcat
    17161 cccagcagtc gagtgtatac aaaaaatgat tggaaccgct tgcccgcgtg cagtattcca
    17221 tattttgctg gactgccagt attggcttct ctataaaagg atggcagcct ataatttccc
    17281 cgcctgtttc gcaactcctg ggaatcgcca tcactgtcac ttgtgctgtc ttacctccgc
    17341 tgtcggtcgc cttgtaccgt atctggtaat aaccgaaaga cgccgtccag ggctcctgat
    17401 ttgaaacaga aactaatttc agcactttat tttctgaatc atacatgtaa acataaacta
    17461 acattgagac agtaagaggg ttcttatcac tgtcaacagc agtcacagag aggggacctg
    17521 ctgctgccca gtctgctccg cattctattg agtcgtaaac aaaaacaggc tcatcaagca
    17581 aagaaatatt gcaataacct ccg
//
LOCUS       DAOPWB010000063        17601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3272_length_17601_cov_4.786219, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000063 DAOPWB010000000
VERSION     DAOPWB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17601
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3272_length_17601_cov_4.786219"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PKN92_07375"
     CDS             complement(<1..968)
                     /locus_tag="PKN92_07375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNZ48651.1"
                     /translation="MPGLLEPQNFYPGRVAMGAEKVRRGVVRLLSAVISRLKPEYEGG
                     SFWLLTSGVLRIVILLLASLGTQRVFQPDIALWLLTGLFIAAMACGIWFLIVVRFQGK
                     VSPALVWTQVLLDFTIVAATLSYTGGHGSYFSFLFVVIILEAGMLMGFFHGFVFAFLS
                     MTYILTLQPPDPFKAVFALPYWYSLLLQGTALFITALISGQWNERINRLKRFQREILD
                     NMVSGFLFCDLDGKISIANKAACAILDIDEASLVGQNATRTPLCGAGKEYPVVTALQT
                     GSDYINYEYSVETKGGRTKVLELTTHKMLGRTGRMQALIVTFKDIT"
     gene            complement(984..2057)
                     /locus_tag="PKN92_07380"
     CDS             complement(984..2057)
                     /locus_tag="PKN92_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNZ48652.1"
                     /translation="MAAPHIRELLVKMVEEEASDLHIVVGAPPMVRINGSVEPLKGYA
                     RLNAHDTQELVYSVMNEEQIAEFESEKECDLSFGVEGLSRFRLNVYLDRGSVVGAFRS
                     IPYTILSFEELGLPRVIAEFAHKPVGLVLVCGPTGSGKSTTLATIINKINTERKTHII
                     TVEDPIEYLHRHRMSIINQREVHSDTKSFNAALKRILRQDPDVILIGEMRDPETIQAA
                     LTVAETGHLAFATLHTNDALQTINRIVDVFPSAQQSQIRTQLSFVLEGVVVQQLIPRA
                     DGLGRVIALEVMLPNPAIRSLIRSEKLEQIPSMIEIGKAEGMMTMNQSLYRLLRRGII
                     TMDMAFKRSFDPEGLQNLINKGL"
     gene            complement(2183..3913)
                     /locus_tag="PKN92_07385"
     CDS             complement(2183..3913)
                     /locus_tag="PKN92_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNZ48653.1"
                     /translation="MAYKIRKERLPDMLLAEGLVNKAQLSECVEIHRNTGQSLPSLLV
                     GMGYLDEEDLAGTLSDNLGIPHIRVASCNVSKEVLKEVPESLARQYQMLPVSITGGVL
                     TLAMADPLNIMAIDDLRMVTKYDIEPVVGIMSELRAAIDRFYGGSSEDQLLSTIMGKN
                     AERFEEIMPVQDDEPRENLSAITKGAEIEPVRELVRLILKSGLDQGASDIHIEPFEKL
                     VRVRYRIDGQLEEATIKPPKNMQPNLIARLKILSGCRIDEHRLPQDGRARMFYNDREI
                     DFRVAFLPCKYGEKVVLRILDQGSLTLDLDSLGFEPQPMEAFRFALDMPHGMILLTGP
                     TGSGKTTTLYSALHRLNTMETNIVTVEDPIEYELFGVNQVQVRQRIGFTFAEALRQIL
                     RQDPDVVMVGEIRDGETADVAVKAALTGHLVLSTLHTNDASGVFPRLIDMDVEPFLVQ
                     SSVALAAAQRLLKRLCNECKQPVDIPEEVLQRVQYTPFSYIEKPQFYNATGCSKCKET
                     GYKGRVAVVEVMLNWPELQPLVLGRASGHEIKEQAIACGMKTLRQNSLSKAAQGVTTI
                     QQVLEHTIAD"
     gene            complement(4034..5026)
                     /locus_tag="PKN92_07390"
     CDS             complement(4034..5026)
                     /locus_tag="PKN92_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48654.1"
                     /translation="MTDSIRWGILGTGTIARKFAEGLRFAKGAELRAVGSRSMETAVQ
                     FAEQYQVPNRHACYESLAADPEVDVVYIATPHPMHKNNSMLCLNAGKAVLCEKPFTVN
                     REEAEALADCARETGRFLMEAMWTRFIPVICEVRRLLREGVLGQVRLIRGDFGFCGDY
                     EEDKSAFLPELAGGALLDVGIYPLSLASMVFGAYPAKVLSAATFGKYGTDELNAVTLV
                     YPDGGLALVSSAIRLETPQDAVITGDKGSIHIHAPFWCAEKITVSVGGSAPEVIEMPL
                     YGNGYNYEAEAVMEAMREGRLESPVMPLYESIALVALMDEIRAQWKLRYPVENT"
     gene            complement(5084..6565)
                     /locus_tag="PKN92_07395"
     CDS             complement(5084..6565)
                     /locus_tag="PKN92_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNZ48655.1"
                     /translation="MLRTPKRLIPAALILFWLIMAGLLVYREAVVPRLYHGGTWKRAA
                     MPEDIWLGLFFGEDQLAGSVHLQTVPSDRNDEEGTSLKASVYLDLPLFGKKTHFALTG
                     TAWQSALYGLREFDFALRAEEHDMRLTGTVDAGQINAVMHTAGEEVPFTLPVGSKLLL
                     SGGLGLSAVTLPHMQPGEVVYLDAFDPATMSLGKARLEALRHEVLQVAGEAVETTLIE
                     TSIGGIVTRAWVSKDEEIVRAETPLGLIIKKISPEDVLKAEEREDSGVAREALTVRAK
                     GLPVAKDMNRLVLRISGLNETLNLPEDSRQHREGDLWVFERQEPGKAAATEPFTASDA
                     DEYLAPDIFIQSSHPRIQEMAASIAGETEDPWIAAVQLHDWVYEHVKKVHVLSVPSAL
                     EVLERLEGDCNEHAVLYVAFARALKIPSRVAIGLVWSDELNAFGYHAWPEVYTGRWIA
                     LDPTFGERYASPTHIKLFTGSIDQWARLMAFVGKIEIEVVSVS"
     gene            complement(6541..9225)
                     /gene="secA"
                     /locus_tag="PKN92_07400"
     CDS             complement(6541..9225)
                     /gene="secA"
                     /locus_tag="PKN92_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNZ48656.1"
                     /translation="MLSSLLQKFFGTSADREIRKLTPLLEQVNARERIYSVMDNDTLR
                     AQTGVFREKLSQGCSLDELLPDAFSAAREAAKRVVGLRPFDVQVLGGIVLHQGGIAEM
                     VTGEGKTLVAILPCYLNALTGKGVHMVTVNDYLARRDAEWMGPVHRFLGLTVGLIQHD
                     MGTYERQAGYRADVTYGTNNEYGFDYLRDNGSQWKSYCVQRELNYAIVDEVDSILIDE
                     ARTPLIISGRPEKSSDMYLRVDGVVRQLRNEIHFEKDEKQRSIILTDDGMVACERLLG
                     IDDLYTDDNIGYMHLIEQSLKAHHFFKRDDEYMVQDDEVLIVDEFTGRAMEGRRYSDG
                     LHQAIEAKEGVPLRFESQTIASITYQNYFRLYSKLAGMTGTALTEALEFEKTYGLRVT
                     QIPTNLPLIREDLSDLIFASEEGKFRYVIKEISRIFDTGRPILVGTVSIEKSELVAKM
                     LTEAGFDDFQVLNAKHHEREAAIVANAGKKNAITIATNMAGRGTDIKLEEGVRELGGL
                     YIIGTERHESRRIDNQLRGRCGRQGDPGTSRFYVSLEDEVARLFGGATAKRFMGKKGG
                     AEMDETPLNMRILSRSIESAQRKVEEYNFEMRKVLLDYDQVMDKQRKYIYGLRNEVLD
                     GKDVTAHLHRMFENIVADMLDLYAPESKLPEDWDMEGLESALHTLTGKTFDLVGDPSE
                     SLQDLVFRQLLEEYRRREQLLAEDIEEQFRERGDDALSVISPAKLARKHFHDLELAEL
                     LRAIDDKWINHLYEMDYLRDSVRLRAFGQKDPLLEYKEEGFELFENLIQSIEETVTQN
                     LFRITDPSRRKRVQPGQSRRSVSGQSEAKAREYQYSGADKESGRSFSDVAAPTVRRGE
                     SKPEAQAPVRVQDKTKPNDPCPCGSGKKFKKCCGRQNV"
     gene            complement(9330..10586)
                     /locus_tag="PKN92_07405"
     CDS             complement(9330..10586)
                     /locus_tag="PKN92_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNZ48657.1"
                     /translation="MPFRKGSATNRGRLISRIALVQKVEWSLVAVRYLACIACGAYAV
                     MDSGPGIVRAVALFCLIALLHNGFVHWVMYRKQYKLFTSLLNFFIYLVFCCVLIGMTG
                     GSASYFAPLLLFVIVGYHIYKPQSGNTLWVTLLVCAAYSFTIVLSWFTTGFNWLHLPV
                     YVNLAFIATSGGVMGVLSQLLYVLEKETQHHAAALESSEETLRAILNHTAHPIVVYDD
                     NELITEVNDSACNFLSMSRLNMIGMRFQSFLFDDGSLGEALLELKQSGSLFQEMLIVP
                     SHGSERAVLMHIHSFLGEKKRFFVALFHDITEQKELNETHRIAKQRLEEANRELHRAA
                     EIRDAFYTSVANQLRSPLAAMLGYIDMLLEEHLGTINPEQREALYSCRRSLHRIFERL
                     DEAFIAYPAEKTDEDAELSEDLGSGI"
     gene            complement(10602..11777)
                     /locus_tag="PKN92_07410"
     CDS             complement(10602..11777)
                     /locus_tag="PKN92_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNZ48658.1"
                     /translation="MSSGKDPYILITNRRSWERCLKELQEAPRIAVDLEANGLYAYRE
                     KICLIQISTDRNHFIVDPLAGFSLEGLKPIFADESVEKVFHAVDYDLTLLQGLYRWKV
                     RGLFDTMRAAEILGHTSIGLASLLGSFYGLTLSKKYQKANWGLRPLSREHLLYAYNDT
                     CHLLRLRDDLATHLVRENLMEEAREAFEQVCHMPPAMRVFDPDHFWKIPGALHLDDPL
                     PAVLKALFIFRESEAQKQDVPSFKIMSNKMLAGIIQQVGTWHKTGVRELVPDACIPLK
                     LLERYRAVLMKTIQAALLHLPPPRISCQSIHTNGFWQRLEKLKEWRKNEADGQGVQSN
                     VVLTRAAMTEIAERNPGTLQALSMLKLLGPVCCRKYGPDILEVLRSASEEAPSNKQT"
     gene            complement(12068..16978)
                     /locus_tag="PKN92_07415"
     CDS             complement(12068..16978)
                     /locus_tag="PKN92_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNZ48659.1"
                     /translation="MTKKSENQDQEKVQQLLKYLLEVMKLRSAVIYDLTSYEAVLWIS
                     DIPKEKGCSTTLWSASPNNETDICIEIEKQEEPAMPAVPASCENWINSTELQDKTTVP
                     ELMPKIIQEQPNPNWTEDSDEEQTVYKTLYLGEHAKIQEDWERYVEGSWKEWREKHNR
                     WEAGFKIYSMLFKIHQDQLRLGEEHELVLGYGLLLWVNPQEKRIYRHLAVANVSLEFD
                     TARAKFSVRTHHEGAKLRAELEMLGQSIDSAEIKKAEMLFSDAGDDLSDHTTIFSAVK
                     GLGEALSAQCRIDESFDRGKEKPSNTPKIIWSPALILRKHSTKGISEALQSMMKKIEL
                     DTTLPSGLAALAELDQSNNIEVRQSSDNTASPETSEIFFPLQANTEQFQIIKKLQHSS
                     GLLVQGPPGTGKSHTIANLICHLLATGQKLLITAKKAHALEVLYKKIPKDLRPLNINL
                     LGGGLSERQSLETSVSCIIARKSAWNFEKEQKKIAELREELKKAREEQEKIRKRMEAV
                     RMSETNSDNRLGDGRYRGAAASITRKVTEEKEYFNWFVDKVPLEGLCPLQTEQLSDLL
                     RSFKFLQSLPEEKKKELHQFLPNDLQSVDTCIAFFEEEKKLAEQEMSLEGKVRSDSAH
                     ALSLNAKELVQKIVGCCQKIESVSGAIKQAMPWLKKELPNILAGTDNFWPRLLKETEK
                     RDRIPENNRRFVDRCSLDYPGSKSRSLGHDAKKLLEHLKQGKKLKFLGIPHRAIKEQI
                     YITKSVILNGKPCITTEDLQKLIGLLNIKHIYSSIWAQWSGILEAPSSSLENQERILA
                     SAYENLKTALHYKQSIDALKKLVPPELSSSLPDWCTPEEVEVFSSSCKMALVQIQKKE
                     VVKKIQIIEAQISKYLLQENVHHLLKDLKASIQERDQLRLTYALEKIGEAQREKSKYA
                     SAIEKIDSLKRDLPVFTQSLKATLSEEVWEQRMESLCDAWYWRQAYDWIFEFTKGDSF
                     QELNDEFKKKEELIHRRIEQLASSYAWSYCCENLKEKHRKHMISWQGAMKKLGKGTGK
                     NAPIHRRAAQEHLDQCRAAIPSWIMPLDRVWDTIAPEAAMFDVVIIDEASQCGLDALP
                     LLYLGKKILIVGDDKQISPYSVGVNEPDVQSLMQLHLKDFAHRDSFRIDSSLFDHGNR
                     LYPNRISLREHFRCMPEIIEFSNQLCYQSSSLIPLRQYGADRLVPLKSVYVNDGLREG
                     RDAGVYNENEAQAVADCIKEMCRDDKYKGKTMGVIVLQGQAQGQLIQDKLFKMLGAEE
                     IERRKLLCGNPYSFQGDERDIIFLSLVAARNQTIGSLTTEVYIRSFNVAASRAKDMML
                     LFHSVKPEDLSSLDLRRKLLEYFLNPTIRSVAGIQLHVLEKQAKDTNRSIANAPDPFE
                     SWFEVDVALELLRKNYSVVPQFPVAGKKIDLVVQGGKAALAVECDGDHWHGPEKYEED
                     MGRQRILERCGWEFFRIRASLFYFDKELALERLWPLLEERQIFPLKAGEEAEQSVGKN
                     MYLESGRAEKEKSSKQKSLYPEEVDSCIRAVSVTPVTKSENDQPVDEQGAVFEKKGIL
                     KISDISKEDLESCILYIGKLRPNNTCKLDDVVTLVLKEFRIKTSGEPRKKFDARVKKM
                     VRNLEERGLVEIYHAKNKRVRFLYPV"
BASE COUNT         4393 a         4708 c         3980 g         4520 t
ORIGIN      
        1 tccgttatat ctttaaaggt tacaataagc gcctgcatac gaccggtacg acccagcatt
       61 ttatgcgtgg tcagttcgag aactttggtt ctcccgcctt ttgtctccac tgaatattca
      121 tagtttatat aatcactgcc ggtctgcagc gccgtgacaa cgggatactc tttgcccgct
      181 ccgcacagcg gtgttctggt ggcattttga ccgaccagag aagcctcatc tatatccaga
      241 atggcgcatg ccgctttgtt ggcgatgctg attttgccgt caagatcaca gaaaagaaag
      301 ccgctcacca tattgtccag tatctcgcgc tgaaaccgct tcaggcgatt aatccgctca
      361 ttccattgtc cgctgataag cgccgtaata aaaagagcgg tgccctggag caacagggag
      421 taccaatagg gcagggcaaa taccgcttta aaaggatcgg gcggctgaag cgtcagaata
      481 taggtcatgg aaaggaatgc aaaaacaaag ccgtggaaaa aacccatgag cattcccgct
      541 tcaagaatga tcaccacaaa aagaaaactg aaataacttc cgtgtcctcc tgtataactg
      601 agcgtggcgg caacaatagt gaagtccaga agcacctgag tccagaccag tgcggggctt
      661 accttaccct gaaaacgcac aacgatcaga aaccagatgc cgcatgccat ggcggcaata
      721 aaaagccccg tcaaaagcca cagggcaatg tcaggctgaa aaacgcgctg tgtgcccaga
      781 ctcgccagca gcaaaatcac gatgcggagc acaccgctcg tcagaagcca gaaagaaccg
      841 ccctcatatt ccggcttcag tctgctgatg acagcgctca gcaggcgcac gactcctctt
      901 cgcacttttt cagctcccat ggcaactctg cccggataga agttctgagg ctccaataag
      961 cccggcatac gccaaagccg atgctacagc cccttattga tcagattctg aagcccttcc
     1021 ggatcaaagc tgcgcttaaa tgccatgtcc atagtgatta tcccgcgccg cagcaggcgg
     1081 tacagcgact gattcattgt catcattcct tccgccttgc ctatttcaat cattgacgga
     1141 atctgctcca gtttttccga gcggatcaga gaacgtatgg cgggattggg cagcatgact
     1201 tccagggcga taacccgacc gagaccgtcg gcgcggggga tcagctgctg gacaactacc
     1261 ccttccagca caaaagacaa ctgggtgcga atctgggact gctgtgccga cgggaacacg
     1321 tcgacaatac ggttgatcgt ttgcagggca tcgttggtat gcagtgtagc aaaggccaga
     1381 tggccggttt ccgcaacggt cagggcggcc tggatcgttt caggatcgcg catctcgccg
     1441 ataaggataa catcggggtc ctgccggaga atacgtttca gcgccgcatt gaaggacttg
     1501 gtgtccgaat gcacttcgcg ctggttaatg atggacatgc gatgccgatg caggtattca
     1561 ataggatctt ccaccgtgat gatatgcgtc ttgcgctctg tgttgatctt attgataatg
     1621 gtggcgaggg tcgttgactt gccgctgccg gtgggaccgc aaacaagcac cagacctacc
     1681 ggtttatggg caaattcagc aataacccgg ggaagtccca gctcttcaaa agaaagtatt
     1741 gtatagggaa tggagcgaaa agcaccgaca acggaacccc tgtccaaata aacattgaga
     1801 cgaaagcggc tcaatccttc cacgccgaaa gaaaggtcac attccttttc tgattcaaat
     1861 tcggctatct gctcctcatt catgacgctg tataccagtt cctgagtgtc atgagcgttc
     1921 agccgcgcat accctttcag cggttcgaca cttccgttaa tgcgcaccat gggcggtgct
     1981 ccgacaacga tatgcaggtc gcttgcctct tcttccacca ttttgacaag cagttcccga
     2041 atatgaggtg cagccatgat tagtccttcc gtttttttat gttaaatcag cgcacagcca
     2101 taaagccgtg atgacagaaa agtagttttt acgcacaaac cgccggcata caacctgacc
     2161 gtccgtataa ccccgctttc catcagtcgg caatcgtgtg ctccagaacc tgttgtatcg
     2221 ttgtaactcc ctgagcagct tttgacaacg aattctgacg cagtgttttc atgccgcagg
     2281 caatcgcctg ttccttgatt tcgtgccccg atgcgcggcc aagcaccagc ggctgcagtt
     2341 ccggccagtt cagcatcact tccaccacag caacacgccc tttgtacccc gtctccttac
     2401 attttgagca gcccgtcgca ttgtagaact gcggcttttc aatataggaa aaaggcgtgt
     2461 actggacgcg ttgcagcact tcctccggaa tgtcgaccgg ctgcttgcac tcgttgcaga
     2521 gccgtttcag cagacgctgc gccgctgcca gcgctacaga cgactgcacg aggaaaggct
     2581 ccacgtccat atcaatcaga cgcgggaaca caccggaagc atcgttggtg tgcagcgtac
     2641 tcagaacaag gtgacccgtc agggcagcct ttactgctac gtccgctgtt tcaccatctc
     2701 gaatctcacc caccataacc acatccgggt cctgacgcag aatctgacgg agcgcctccg
     2761 caaaggtaaa tccgatccgc tggcgcacct gcacctgatt caccccgaaa agttcatatt
     2821 caatcgggtc ttccacagtg acgatattgg tttccatggt attcaggcgg tgaagcgcgc
     2881 tgtaaagcgt cgttgttttt ccgcttcctg taggtcctgt cagcaggatc atgccatggg
     2941 gcatgtccag tgcgaacctg aaagcttcca tgggctgcgg ttcaaaacct aacgaatcaa
     3001 ggtccagcgt gaggctgccc tgatccagaa tacgcagcac caccttttcc ccgtatttac
     3061 aggggaggaa ggcaacacgg aagtcgattt cacggtcatt gtagaacatg cgggccctgc
     3121 cgtcctgagg cagacggtgt tcatcaatac gacagcccga caaaatcttg agccgtgcga
     3181 taagattggg ctgcatgttc ttcggcggtt tgatggttgc ttcttcgagc tgtccgtcta
     3241 tgcggtagcg gacacgcaca agcttttcaa agggttcaat atgaatatcg ctggcgccct
     3301 gatcaagccc gcttttcaga atcagacgga ccagctcgcg aacgggctcg atttccgcgc
     3361 ccttggtaat tgcagacaga ttttcgcggg gctcatcatc ctgaaccggc ataatttctt
     3421 caaaacgctc tgcattcttg cccatgatcg tgctgagcag ctgatcttct gaagaaccgc
     3481 cgtagaagcg atcgatggca gcgcgcagtt ccgacataat acccactacc ggttcaatgt
     3541 cgtacttggt caccatgcgc aaatcatcga ttgccatgat attgagcgga tccgccatgg
     3601 caagggtaag cacgcccccg gttatggaca caggcagcat ctgatactgc cgcgcaagag
     3661 attcaggcac ttccttcagc acctctttgg agacgttgca gctggctaca cggatatgag
     3721 gaatgccgag gttgtccgat aaggttcccg ccagatcctc ttcgtcaaga tagcccatgc
     3781 ccacaagcag tgaaggcaga ctctgcccgg tgttacggtg aatctccaca cactccgaca
     3841 gctgcgcctt gttgacaagc ccctccgcaa gcagcatatc cggaagacgt tccttacgaa
     3901 tcttgtacgc catggcttta ctctctcact ctatttaata cggcatgctt attatactgt
     3961 atttcatatg aaatatcaga ccttttttgc cggatgaccc agcagagaaa gtattcttcc
     4021 caaaaccacg gtctcacgtg ttttccaccg gatagcggag cttccattgc gcgcgaattt
     4081 catccatcag cgcaaccagc gcaattgact cgtacagcgg cataaccggc gactccagtc
     4141 tgccttcacg cattgcctcc atcaccgctt ccgcttcgta attatatccg tttccgtaca
     4201 gcggcatttc aatcacttcc ggagcgctgc cgccaacgct taccgttatt ttttcggcgc
     4261 accagaaagg tgcatgaata tggatggatc ctttgtcgcc ggtaataacc gcatcctgcg
     4321 gcgtctccaa tcgaatggca ctggaaacaa gggcaagacc accgtcgggg taaacaagcg
     4381 taactgcatt caattcatct gtcccgtatt taccgaaagt tgccgctgaa agtacttttg
     4441 ccggataggc cccgaaaacc atggaagcca aagacaacgg ataaataccc acatcaagaa
     4501 gcgcaccgcc tgcgagctcg ggaagaaatg cacttttgtc ttcttcataa tcaccacaaa
     4561 aaccgaaatc accccggata agccgcacct gtccgagcac gccttcccga agcagccgac
     4621 gcacttcaca aatgaccgga ataaagcggg tccacatggc ctccatgaga aagcgccccg
     4681 tttcgcgcgc acagtctgcc agcgcttccg cttcctcccg gttaaccgtg aaaggctttt
     4741 cgcaaagaac agccttgcct gcgttcaggc agagcatgga attgtttttg tgcatgggat
     4801 gcggcgtggc tatatacacc acatcgactt ccggatccgc tgcaagggac tcgtaacagg
     4861 cgtgacggtt aggcacctga tattgttcgg cgaactgcac cgccgtctcc atcgagcgcg
     4921 accctacagc acgcagttcc gcccctttgg cgaagcgcag accttcggcg aatttccggg
     4981 caatggtgcc tgtgccgaga ataccccagc ggatgctatc tgtcatcttt ttgtttcctt
     5041 tgttctgtcc tgaaaagatt tttcttccgg caacagcact gcttcagctc acggagacaa
     5101 cttcaatctc tatctttccg acaaatgcca taagccgcgc ccattggtct atgctcccgg
     5161 taaacagttt tatgtgagta ggggaagcat aacgctctcc gaaagtggga tcaagagcaa
     5221 tccagcgccc cgtgtacact tccggccagg catgatatcc gaaagcattg agctcatcgc
     5281 tccagacaag accgatggcg actcgcgacg gaattttcaa cgcacgggca aacgccacat
     5341 aaagcacagc gtgttcattg cagtcgcctt caagccgttc aagcacttcc agcgcgctgg
     5401 gaacggaaag cacatgcact ttcttcacat gctcgtagac ccagtcatgg agctgaacag
     5461 cagcgatcca ggggtcttct gtttcgccgg caatggatgc cgccatttcc tggatccggg
     5521 gatggctgga ctgaataaaa atatcgggag caagatattc atccgcatcc gatgcggtga
     5581 aaggttccgt cgccgctgcc tttccgggtt cctgtcgttc aaagacccac agatcgccct
     5641 cacgatgctg acggctgtct tcaggcagat tcagcgtttc attcagcccc gaaatacgga
     5701 gaaccagacg gttcatatcc tttgcgacgg gaagcccctt tgcccggaca gtaagtgctt
     5761 cacgggcaac accggaatct tcacgctctt ccgcctttaa aacatcttcc ggcgatatct
     5821 ttttaataat cagccccaag ggcgtttcag cgcggactat ttcctcgtct ttggaaaccc
     5881 atgcgcgcgt tacaattccg ccaatgctgg tttctataag tgtggtttcc acagcctccc
     5941 ctgccacctg cagcacttca tgccggaggg cttcaagccg ggcttttcca agagacatgg
     6001 tcgccgggtc aaaagcatcc agatacacga cttctcccgg ctgcatgtgc ggcagcgtga
     6061 cggcagacaa ccccaacccg ccggacagaa gcagtttgct ccccacgggc agcgtaaacg
     6121 gcacctcctc accggctgta tgcatgaccg catttatttg acctgcgtct accgtacccg
     6181 taaggcgcat atcgtgctct tcagctcgaa gcgcgaaatc aaattcacga agtccataca
     6241 gggcagactg ccaggctgta cccgtaaggg caaaatgggt ctttttcccg aagagcggca
     6301 gatcaagata aaccgacgcc ttgagtgaag tgccctcttc gtcgtttctg tcggaaggta
     6361 ctgtctgcag gtgaacagaa ccggcaagct gatcttcccc gaaaaaaaga ccgagccaga
     6421 tatcctccgg catagcagcc cgcttccacg tgccgccatg gtacaggcga ggcacaacgg
     6481 cctccctgta cacaagcagt cctgccatga tcagccagaa aaggatgagc gcagccggaa
     6541 tcagacgttt tggcgtccgc aacacttctt gaacttcttt ccgctaccgc aaggacacgg
     6601 gtcattgggt ttggttttat cctgaacacg gacaggggcc tgcgcctcag gctttgattc
     6661 cccccgacgc accgtcgggg cggcaacatc ggaaaaactg cgccctgatt ctttgtcggc
     6721 gccactgtac tgatattccc gcgcttttgc ctcactctgc ccggagacac tgcgccggga
     6781 ctggccgggc tgcacccgtt tacgccggga cgggtcggtg attctgaaaa gattctgggt
     6841 gaccgtctcc tcgatggatt gaatcaggtt ttcaaacagt tcaaaaccct cttccttgta
     6901 ttccagcaga ggatctttct gaccgaaggc gcggagacgc accgaatcgc gcaggtaatc
     6961 catctcataa agatggttga tccatttgtc atcaatggcg cggagcagtt cggcgagctc
     7021 cagatcatga aaatgtttgc gcgccagctt ggcagggctg atgacagaaa gcgcatcgtc
     7081 tccccgctcc ctgaactgct cttcgatgtc ctccgccagc aattgctccc gacgcctgta
     7141 ctcttcaagg agctgacgaa acacaagatc ctgcagagat tcactgggat cgcctaccag
     7201 atcaaaggtt ttgcctgtca gcgtgtgcag tgccgattcc agcccttcca tatcccagtc
     7261 ttcaggcaat ttgctttcag gcgcatagag atccagcata tccgcaacaa tattttcaaa
     7321 catgcgatgc aggtgggcgg ttacgtcctt cccgtccagc acctcattgc gcagtccgta
     7381 aatgtatttg cgctgtttgt ccatgacctg gtcatagtcc agaagaactt tgcgcatctc
     7441 gaagttgtat tcttccactt tgcgctgcgc cgactcaatg gatctggaga ggatgcgcat
     7501 attaagcggt gtttcatcca tttccgcacc gccctttttt cccatgaagc gctttgccgt
     7561 ggcaccgccg aaaagacgtg ccacttcatc ctccaggctc acataaaaac ggctggttcc
     7621 cggatccccc tgccttccgc agcgaccccg gagctggttg tcgatccggc gtgattcgtg
     7681 ccgttccgta ccgatgatat acagcccgcc cagctcgcgg acaccctctt caagtttgat
     7741 atccgtgccg cgccctgcca tgttggtggc aatggtgatg gcgtttttct tgccggcgtt
     7801 ggcaacaatt gccgcttccc gttcatgatg ctttgcattg agcacctgaa aatcatcaaa
     7861 gcctgcctcg gtcagcatct ttgccaccaa ctctgatttt tcgatggata ccgtgccgac
     7921 aagaatggga cgccctgtgt cgaagatacg ggagatctct ttgatcacat agcggaattt
     7981 tccctcttcg gaagcgaaaa tcaggtcaga gaggtcttcc cggatcaggg gcagattggt
     8041 cggaatctga gtcacccgaa gcccgtaggt tttttcaaac tcaagtgcct cagtcagggc
     8101 ggttccggtc atgcctgcca gcttcgaata aagccgaaag taattctgat aggtaatgga
     8161 ggcaatagtc tgcgattcaa agcggagcgg cacgccctct tttgcctcaa tcgcctgatg
     8221 cagaccgtcg gaatagcggc gtccttccat ggcacgaccg gtgaactcat ccacaatcag
     8281 cacttcatcg tcctgcacca tatactcgtc atcgcgtttg aaaaaatgat gagctttcag
     8341 cgactgctca atgagatgca tatagccgat gttgtcatcc gtataaagat catcgatgcc
     8401 gagcagtcgc tcacaggcaa ccattccgtc atccgtcagg attatgctcc gttgtttttc
     8461 gtctttttcg aaatggattt cattgcgaag ctgccgcaca acaccatcca cgcggaggta
     8521 catgtcgctg cttttttcag gtctcccgga aatgatcaga ggcgtacgcg cttcatcgat
     8581 gagaatggag tccacctcgt ccacaatggc gtagttcaat tcgcgttgaa cacaatagga
     8641 tttccactgc gaaccattat cacgaaggta atcaaagccg tattcgttat tggtgccgta
     8701 ggtcacgtcg gcacgatacc ctgcctgacg ctcatacgta cccatgtcat gctggatcag
     8761 tcctaccgtc agcccgagaa aacgatgcac cggacccatc cattcagcgt cacggcgcgc
     8821 cagataatcg ttgaccgtga ccatatgcac acccttgccg gtaagggcat tcaaatagca
     8881 gggaagaatg gcaacgagtg tcttgccctc gccggtaacc atctcggcaa tgccgccctg
     8941 atgcagcacg atcccgccca gaacctgcac atcaaagggg cgcagcccca caacacgttt
     9001 tgccgcttcc cgggcagcag aaaaggcatc cggaagcagt tcgtccagac tgcagccctg
     9061 actcaacttt tcccggaaaa ccccggtctg ggcacgcagt gtatcattgt ccatgacact
     9121 gtaaatacgt tcccgggcgt tcacctgctc aagaaggggc gttaatttcc ttatttcgcg
     9181 gtcggcactg gtgccgaaaa atttctgtag taagctggaa agcaaagaga aaggtctcca
     9241 atgggtttgc gcacggttat attgttgtga ttgcgatgac gcaatgcaaa taaaatggat
     9301 cgccgggcgg tgtgcctgat gacagggcgt cagatgcccg agcccagatc ttctgataat
     9361 tccgcatctt cgtctgtttt ctctgcgggg tatgctatga acgcctcgtc cagccgctca
     9421 aagatgcggt gaaggctgcg acggcagctg tacaaagcct cacgctgttc gggattgatc
     9481 gtgccgaggt gctcttccag cagcatgtct atgtacccga gcatggcggc aagaggggag
     9541 cgcagctggt tggcaacgga tgtatagaag gcatcgcgta tttccgccgc acggtgaagt
     9601 tcacggttgg cctcttccag ccgctgtttg gctatgcggt gcgtttcgtt caactctttc
     9661 tgctcggtta tatcatggaa aagcgccaca aaaaaccgtt tcttttctcc gagaaaagaa
     9721 tgaatgtgca ttaaaaccgc acgctccgaa ccgtgggaag gcacaataag catttcctga
     9781 aaaagggatc ccgactgttt gagttccaga agtgcctcgc ccagtgaacc atcatcaaac
     9841 agaaaactct gaaagcgcat accgatcata ttcaggcgcg acatgctcaa aaaattacag
     9901 gcactgtcgt tgacttccgt aatcagctca ttgtcatcat aaaccactat cggatgcgcg
     9961 gtatggttca gaatggcacg cagcgtttct tccgaacttt caagcgccgc cgcatggtgc
    10021 tgcgtctctt tttcaagaac ataaagcagc tgcgaaagaa cgcccataac accgccactg
    10081 gtcgcaatga acgccagatt cacatatacc ggcagatgca gccagttaaa gcccgtggta
    10141 aaccagctca gcacaatcgt aaaggaatag gccgcacaga ccagcagtgt aacccatagc
    10201 gtattgcccg actgcggctt gtaaatgtga tagcccacta tgacaaagag aagcaatggc
    10261 gcaaaataac tggcggaacc gcctgtcatg ccgatgagca cgcagcagaa aaccagatag
    10321 atgaaaaaat tcaacagcga tgtaaagagc ttatactgtt tgcggtacat cacccagtgg
    10381 acaaagccgt tatgcagcaa ggcaatcagg cagaaaagag cgacagccct gacgatcccc
    10441 gggccgctgt ccattacagc atacgcgccg caggcgatac acgcaagata gcgcaccgct
    10501 acaagcgacc actccacctt ctgtaccaga gctattcggc tgataagacg gcctctgttc
    10561 gtagcgcttc cctttctaaa gggcatgtct atactcttcc tttacgtctg cttgttcgaa
    10621 ggtgcctctt ccgatgcgga tctgagcacc tcgagaatat ccggaccata cttgcgacag
    10681 caaaccgggc ccaacaattt tagcatggat aaggcctgaa gtgtaccagg gttacgctcc
    10741 gcaatttcgg tcattgccgc tcgggtaaga accacgttcg actgcacccc ctgcccgtct
    10801 gcctcattct tgcgccattc tttcagcttt tcaagccgct gccagaaacc gttggtgtgg
    10861 atgctctgac aggagatgcg tgggggcggc agatgtaaca gcgccgcctg gatggtcttc
    10921 atcagaacgg ctctgtaccg ctccagcagt ttcagcggaa tgcatgcatc cggcaccagt
    10981 tcacgaacac ccgtcttatg ccaggtgccc acctgctgga taatgcccgc aagcattttg
    11041 ttcgacataa ttttaaagga gggcacatcc tgtttttgcg cttccgattc acgaaagata
    11101 aagagtgctt tcagcacggc gggaaggggg tcgtccaggt ggagagcccc cggtattttc
    11161 cagaagtgat ccgggtcaaa tacacgcatg gcaggtggca tgtgacaaac ctgctcaaaa
    11221 gcctcgcgcg cctcttccat caggttttct ctgaccagat gcgttgccag atcatcgcga
    11281 agccgaagca gatgacaggt atcattgtag gcataaagga gatgttcacg ggaaagcggg
    11341 cggagacccc agttggcttt ctggtatttc ttgctcaggg taagaccata aaacgaccca
    11401 agcagcgacg caagcccgat actggtgtgc cccagaattt ccgccgcacg catcgtatcg
    11461 aataaaccac ggactttcca ccggtatagc ccctgaagaa gcgtaagatc atagtctact
    11521 gcatgaaaaa ccttttcgac agactcatcg gcaaaaatcg gtttcagccc ttccagtgaa
    11581 aaaccggcaa gaggatccac gataaaatgg tttctgtccg tggaaatctg gatcaggcag
    11641 atcttttcgc gataggcata aagaccgttc gcctccagat caacggcgat tcggggcgct
    11701 tcttgcagtt ccttaagaca acgctcccag gaacggcggt ttgtaatcag tatgtaggga
    11761 tcttttccgg aggacaccaa aacacaacgc tccataaatc acgacgcaga tcagtataac
    11821 agggcactat acacctctca ctggctttca cagcaaaagt gaaccccccg tctgctcatt
    11881 ttaccataaa tatccccggc agaaaaatct tccgggaaag ccatatggtg cagtaaaagc
    11941 attatcctta ccaatagcga tgcaaaagaa caaaattgtg gcgataccgc tgtttttagg
    12001 attgacagag aaaatgagga aaacaggtaa agactgtggt gtaaaaccct catctaatca
    12061 acacaaatta tacaggataa aggaaacgca ccctcttgtt ttttgcatgg tatatttcta
    12121 ccaagcccct ttcttcaaga tttctcacca tttttttcac ccgtgcgtca aatttctttc
    12181 taggctcccc tgaagttttg atcctaaact cttttaatac aagagtcaca acgtcatcca
    12241 gtttacacgt gttgttgggt cgcagtttgc caatatacaa aatacatgat tccagatctt
    12301 ctttactgat gtccgatatc tttaaaatac cctttttctc aaaaacagcg ccttgctcat
    12361 ctacaggctg atcattttca cttttagtga caggtgtaac cgacaccgcc ctgatgcacg
    12421 agtccacctc ttcaggatat aagcttttct gtttggaaga tttttccttt tccgctctcc
    12481 ctgattccaa gtacatattt tttcccacag attgctctgc ctcttcacca gctttcagag
    12541 ggaaaatttg ccgttcctct aacagaggcc acaagcgttc tagagcaagt tctttatcaa
    12601 aataaaaaag agacgctctg attcggaaaa attcccatcc gcagcgctct agaattcttt
    12661 ggcgccccat atcttcctca tatttttccg gaccatgcca gtggtctccg tcacattcaa
    12721 cggcaagagc agctttgccc ccttgaacca caaggtcaat tttctttccg gccacaggga
    12781 actgcgggac aacactgtaa tttttccgca gcagttccaa ggccacatcg acctcaaacc
    12841 aactctcaaa aggatccggt gcgttagcaa tgctgcggtt cgtatctttt gcttgttttt
    12901 ccagtacgtg caactgaatc cctgcaacag accggattgt tggatttaaa aaatactcga
    12961 gcaacttacg tcgaagatct aaagagctta agtcttcagg ctttacagag tggaaaagca
    13021 gcatcatatc tttagcacgg ctggctgcga cattaaaact tcggatatat acttctgtgg
    13081 taagagaacc aattgtttga ttacgagccg caacaaggga gagaaagatt atatcccgtt
    13141 catcgccctg gaaactgtaa ggatttccgc agagcaattt tcgccgttct atctcttcgg
    13201 cacccagcat tttaaatagc ttatcctgta ttaactgacc ctgggcctga ccctgtaaaa
    13261 cgataacccc catggtcttc cctttgtact tatcgtcacg acacatctct ttgatacaat
    13321 cagccacggc ttgagcttca ttctcattgt aaacaccggc atcgcgtccc tctcgtaaac
    13381 catcattaac atagaccgat ttcaaaggta ccagacgatc tgccccgtat tgccttaaag
    13441 gaatgagaga agaactctgg taacacaact gattgctgaa ttcaataatt tcaggcatac
    13501 agcgaaaatg ttcacgcagt gatattcgat ttggataaag tctattgcca tggtcaaata
    13561 aactgctgtc aatgcgaaaa gaatcgcgat gcgcaaaatc tttaaggtga agttgcatca
    13621 aagactgcac gtctggttcg ttgaccccta cactgtaagg gctaatttgt ttatcatccc
    13681 caacaatcaa tattttttta cccaaataaa gcaacggcaa agcatctaag ccgcattgag
    13741 aggcttcgtc gataatcacc acatcaaaca tagctgcctc aggagcgata gtatcccaga
    13801 cacgatcaag aggcataatc caggaaggaa tggcggcacg gcattgatcc agatgttcct
    13861 gcgcagcacg tctgtgtata ggagcatttt ttccagtccc tttgccgagt tttttcatcg
    13921 ctccttgcca tgagatcatg tgctttctat gcttttcttt tagattttca cagcaataag
    13981 accaggcata ggaagaagcc agttgttcga ttcttcggtg aatgagctct tctttctttt
    14041 taaattcatc atttaattcc tgaaagctgt caccttttgt gaactcaaaa atccaatcat
    14101 acgcctgcct ccaataccag gcatcacaaa gcgattccat gcgttgttcc cagacctcct
    14161 cgctcaaagt tgctttaagc gactgagtaa atacgggcaa atccctttta aggctatcaa
    14221 tcttttctat tgccgaggca tatttactct tttccctctg tgcctctcct attttctcga
    14281 gagcataagt cagccgcaat tgatctcgct cttgaatgga cgctttaagg tcttttaaaa
    14341 ggtggtgaac attttcttgc aataaatatt tagaaatttg agcttctatt atttgaatct
    14401 tttttacaac ttctttcttt tgtatctgca ctaaagccat cttacaggaa gaggaaaaaa
    14461 cttcaacctc ttcaggagta caccaatcag gcaaggaaga gctcaactca ggtggcacta
    14521 gtttttttaa agcgtctatg ctttgtttgt aatgtaaagc agtctttaag ttttcataag
    14581 cagaagcaag aattctttcc tgattctcta aagaactcga cggtgcttct aaaatcccag
    14641 accattgcgc ccagatagaa ctgtaaatat gtttaatgtt aagtagccca attaactttt
    14701 gaagatcttc tgtcgtaatg cagggttttc cgttgagtat caccgatttt gttatatata
    14761 tttgctcttt aattgcccga tgaggaattc ccaaaaactt aagttttttc ccttgtttga
    14821 ggtgctcaag caactttttg gcatcgtgac ccagactccg gctcttagat cccggataat
    14881 ccagactaca cctatcgaca aatcttctgt tgttttcagg gattcggtcg cgtttttccg
    14941 tttcttttaa gagtcttggc cagaaattat ctgtacctgc caaaatattc ggcaactctt
    15001 tcttaagcca cggcatggct tgcttaattg cccctgaaac actctcaatt ttctgacagc
    15061 atcccacgat cttctgaacc aactcttttg cgtttaagga cagtgcgtgc gccgagtcac
    15121 ttcgcacttt cccttctaaa gacatctcct gctctgccag ttttttttct tcttcaaaaa
    15181 atgcgatgca agtgtcaacg ctctgcagat cattcggcag aaactgatgc aattcctttt
    15241 tcttttcttc cggcaatgat tgtagaaatt taaaagaacg caggagatcc gacaactgct
    15301 ctgtctgcaa agggcacagt ccctctaaag gtactttatc gacaaaccag ttaaaatact
    15361 ctttttcttc agtaactttc cttgtgatac tagcagcggc acccctatat cttccatcac
    15421 ctaaacggtt gtcgctgtta gtctccgaca ttcgcactgc ttccatgcgt tttctaattt
    15481 tctcttgttc ctcgcgcgct ttttttaact cttctcgcaa ttcagcgatc ttcttttgtt
    15541 ctttttcaaa attccatgcg ctctttcgtg caatgataca cgagacactg gtttcaagag
    15601 attgacgttc gctaagacca cctcccaata aatttatatt aagaggacgc aagtccttcg
    15661 gtatcttttt ataaagcact tcaagtgcat gggctttctt ggcagtaatc aaaagtttct
    15721 ggcctgtggc caaaaggtga cagattaggt ttgctattgt atgagacttt cctgttcctg
    15781 gcggcccttg aaccaaaagc ccgcttgagt gctgtagttt tttgataatt tgaaattgtt
    15841 ctgtattcgc ctgcaacgga aagaaaattt cactagtctc tggcgaggca gtgttatcag
    15901 aggattgtcg tacttcaatg ttatttgatt ggtcaagctc tgctaaggcg gccaagccac
    15961 ttggaagcgt ggtgtcaagt tcaatttttt tcatcatgct ttgaagagct tcacttatcc
    16021 ctttggtaga gtgttttcga agtattaggg ctggactcca aataattttg ggagtattag
    16081 aaggcttctc cttccctcta tcaaaacttt catcaattct gcactgcgca gacaaagcct
    16141 cacccagccc cttgactgct gaaaaaatcg ttgtatgatc agagaggtca tcacccgcat
    16201 cactgaatag catttcagct ttttttattt cggcagaatc aatggactga ccaagcattt
    16261 ctagttcagc tcttaatttt gcgccttcgt ggtgggtgcg cactgaaaat tttgcgcggg
    16321 ctgtatcaaa ttcaagagaa acattagcca ctgctaaatg cctgtaaatt cttttctctt
    16381 gtggattgac ccaaaggagc agaccataac ccaaaacaag ttcatgctct tctcccagtc
    16441 ttaattgatc ctgatgtatt ttaaaaagca ttgaataaat tttaaaccca gcttcccaac
    16501 ggttatgttt ttcacgccat tctttccagg agccttcaac atatctctcc caatcttcct
    16561 gtattttggc gtgctcgcca agatataaag ttttgtatac cgtttgttcc tcgtcagaat
    16621 cttctgtcca attcgggtta ggttgttctt gaatgatttt cggcattagt tctggcacgg
    16681 ttgttttatc ctggagctca gtggaattaa tccagttttc acatgatgct ggtacggctg
    16741 gcatcgcagg ctcttcctgc ttttctattt cgatgcaaat gtccgtttca ttgttcggtg
    16801 acgcagacca taatgttgta gaacatcctt tttcttttgg aatatctgaa atccacaaga
    16861 cagcctcata ggaagtaagg tcataaatca ctgctgagcg gagcttcatc acctccaaaa
    16921 gatactttaa gagttgctgt actttttcct gatcttggtt ctcactcttc tttgtcattt
    16981 tctgtccaac ctcaaaatta aatgcttaca aacttctgtt ttcaaaaacc atctttcggt
    17041 ttccgaattt actctaaatg tagactatta ctgctcagcc tttccagaaa acaagaacat
    17101 gttatcataa tcagaacaat ttagcagaaa agcctgaatt gtagattcgt catcaggaaa
    17161 gtaaatccgt aaattgttcg ttttttaaag aatattatct atttactact atttcttata
    17221 taccatatca ttggtgtcca aattaaggcc accatcgaat ccacatagat gaattcattg
    17281 gcatttcggc cagacttttg tcttcattat cgaggtttca ggatcagagt tgctgatcat
    17341 tgcatttgtc tgtccaaatt gcgaaggcct atgcaagacc attctacagc cccaaaatgc
    17401 cataaagaca ggctttcccg ggtgctcagt acatggaaac aggtgtcagc acacagaaca
    17461 agcttgtcca agaaaagctg atttggacaa gagtgatacc atatcaaaaa actcatttgc
    17521 agttttccgt tcgaaacatt tccagaaggg attatctgtg gctcagcaaa ataaaagcat
    17581 tatgccttac ctgtcggtgg t
//
LOCUS       DAOPWB010000064        17557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3280_length_17557_cov_5.352588, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000064 DAOPWB010000000
VERSION     DAOPWB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17557
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_17557_cov_5.352588"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKN92_07420"
     CDS             complement(<1..245)
                     /locus_tag="PKN92_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNZ48660.1"
                     /translation="MKVLSTQEMREADRRCIEELGMPGCVLMNNAGTAAFKYVKDGPV
                     VVVCGKGNNGGDGFVIARLALLAGLPTRVLLLAHPED"
     gene            complement(269..1639)
                     /gene="dnaA"
                     /locus_tag="PKN92_07425"
     CDS             complement(269..1639)
                     /gene="dnaA"
                     /locus_tag="PKN92_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNZ48661.1"
                     /translation="MTDTAQNPWQNILNDLQVSLDDHCYKNWFSQTSFDSLEDDIIRV
                     RVPSGFFADWLKEHYQDAVLEITRALYPQVNHVEYVPEPAEQEKPVLPAPMPARTSPV
                     APAGKSRLKKHFPKNGFNPKYTFERFVIGAGNRFAHAAAHAVLQAPGRAYNPLFLYGG
                     TGLGKTHLMQAVGQGALNIDPSLRVVFISSEEFTNEMIQSIADKETQRFRAKYRRVDM
                     LLIDDIHFISGKEATQEEFFHTFNTLFDAHKQIVISSDRPPKELKKMEERLVSRFEWG
                     LVTDIQAPDLETRIAILQNKAKLENLNVPPEILRYIATYITNNIRELEGALITLFGYC
                     SLTGSKISLEMAENVLREQIGQGRINPITMEQVQRVVADHFDVRLADLRGQSRQRQVV
                     IPRQIAMYLCKTQIPSISLKDIGEQFGGKDHTTVLHACDKIHREIDRNPTIRQTVDQL
                     TKALRS"
     gene            complement(1893..2294)
                     /locus_tag="PKN92_07430"
     CDS             complement(1893..2294)
                     /locus_tag="PKN92_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNZ48662.1"
                     /translation="METPKTLQQGCLSAIDFALKIQADAAELGFDWSDITGALDKVVE
                     EVQELKAALVHNERKEALNELGDLFFSLLNVSRFLDADPLMCLNGTSDRFQKRFALVK
                     QLAYEERCDLKNCTDDQLDCYWERAKKLVAQ"
     gene            2538..4988
                     /gene="gyrB"
                     /locus_tag="PKN92_07435"
     CDS             2538..4988
                     /gene="gyrB"
                     /locus_tag="PKN92_07435"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNZ48663.1"
                     /translation="MTKNAYGAGSIQVLEGLQAVRMRPAMYIGDTATRGLHHLVYEVV
                     DNSIDEALAGFCKKITVTVHIDNSVTVEDDGRGIPVDNHPNPKFKKKSALEVVLTVLH
                     AGGKFDNTSYKVSGGLHGVGVSCVNALSRWLEVEVRRDGHIYAMSFERGVAKGPIETK
                     GRTKSTGTKMTFLPDTDVFEVSEYNAEILLTRLRELAFLNKGIKIVFEDERTDDEAIV
                     MQYNGGIVDYVKYLTRNKEVLNRTPIYMELARDEVEVEIALQYTTAYNEVVSSFANNI
                     NTYEGGTHLSGFRAALTKSLNDYARKNNMFKKGQNTISGDDTREGLSAIVSVRLRSPQ
                     FEGQTKMKLGNSDVQGIVSSLVYEGLQIYFEENPAVANRIINKTLEAARAREAARKAR
                     ELTRRKGALDSIGQAAKLADCSERDASQCELFIVEGDSAGGSVKQGRDRRFQAVLPLR
                     GKVLNVERARQDKMLENKEIRSLITSIGAGFGQDEFDLAKLRYHKIIIMTDADVDGAH
                     IRTLLLTFFFRHMPELIRNGHLFIAQPPLYQLRKGKKIQYVNTENEYQDFLFELALDT
                     VRVQSADAPQEKNIDLKSLGKSIRAAQDRERMISRVNRVYGVEAEELKKCLLLSREKY
                     ISPQTLGYEDLVTLFGSKEVINTSIEQGDLLEEENGRHPGNGNGNGNGGRRPVIRQKN
                     QVDLAFFKSHEFSALLTSNDALTAIGEPPYQVYSLNEKAEAPLFQSEDLLELRDFLLE
                     TGRQGLMVQRYKGLGEMNAEQLLETTLDPRNRVLLEVKAEDEAAADDMFTMLMGDLVE
                     PRKDFIEKHAAEVQNLDI"
     gene            5046..7607
                     /gene="gyrA"
                     /locus_tag="PKN92_07440"
     CDS             5046..7607
                     /gene="gyrA"
                     /locus_tag="PKN92_07440"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNZ48664.1"
                     /translation="MDQNDRIKPIAIEQEMRSSFLDYSMSVIVSRALPDVRDGLKPVH
                     RRILFAMHNLGLVKNRPFRKCAAIVGDTMGKYHPHGDSSIYDTLVRMAQDWSMRYPLV
                     DGQGNFGSIDGDSAAAMRYTESRMEAITALMLADIDKQTVDMMSNYDGREQEPTVLPS
                     AIPNLLVNGSYGIAVGMATSCPPHNLTEVCDALIHLIDNPEASPKDLMRFVKGPDFPT
                     GGTICGRRGIFDAYTTGRGKVTVRAKAVIEARKDGSQAKIIIQEIPYQVNKTRLIESI
                     AELVHKKIVEGISDLRDESDKDGMRVVIEVKKGEEPEVVLNQLYKHTQLQATASIIML
                     ALVNNAPRVLNLRDMLFFYLEHRAEIITRRSRFELAKAEARAHIVEGLLKASDHIDEV
                     IKIIRASQDDEIARQRLMDRFEFSIPQANAILAMRLRNLTGLKQAELEEEYRDLLKEI
                     ERLRYILSSEKTIMAEVRKEVLEVKEKYGDGRRTEILGDVGEFKIEDLIADENMVVTF
                     SNEGYIKRLPVASYRKQRRGGKGITGMGTKEDDFVRDLFIASAHQYILFFTSLGRVHW
                     RKVHELPQVGRTAKGRNIVNLLQLEKDEVVTAYLPVRNLKEEGHYVFMVTRQGVVKKT
                     PLVQFSNVRTGGIIALTLKEGDVLLDTLITTGDDKILIATNQGKAIRFLESDVRPMGR
                     TAAGVIGMKLQPDDFVVGVSLAKDDRTVLSVTENGYGKRTKVGEYRLQHRGGQGTIDI
                     KTGERNGNVVAMLTVDDDDDLVLISTDGTVIRTGVSGIRTIGRNTLGVKIMNPQPGAV
                     VTAVARAIGDKKEEDATESASDETTEAPEDEGVGYIEQMDFEEDLEDSVEEDTEE"
     gene            complement(7610..7684)
                     /locus_tag="PKN92_07445"
     tRNA            complement(7610..7684)
                     /locus_tag="PKN92_07445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7649..7651),aa:Arg,seq:cct)
     gene            8175..9857
                     /locus_tag="PKN92_07450"
     CDS             8175..9857
                     /locus_tag="PKN92_07450"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HNZ48665.1"
                     /translation="MKEKYVLGLDYGTNSARAVVVSAKTGAEIGTAVFEYPSGEKGII
                     LDEKNPDLARQHPWDYLQALAAIVPAALKEAESVPGFSVDDIIGIGLDATGSTPLPVD
                     KNGTPLALLDDFSKNPAAMAWLWKDHTGHAEAVEITALAEKMRPQYLAKCGGAYSSEW
                     FWSKILYCKRSAPDVFYAAHTWVEHADWMSAVLTGTAHPESLKRSVCAAGHKAMYHQS
                     WGGYPDQEFLTSLDPALGRIRETLPNRAYSIAEKAGGLTEEWALKLGLPTGIPVAMGA
                     IDAHMGAVGSGIQPGVLVKILGTSCCDMLVAPLEQELPDIPGLCGIVPESILPGCYGL
                     EAGQSAVGDIFNWFVNNIRPGGEGHESLTEKALKLKPGESGLLALDWHNGNRTVLVDQ
                     RLTGALLGMTLHTRPEEIYRALIEATAFGARVIMERFLEYGMTVDRIINCGGISGKNA
                     LLMQIYADIMGRPLEVSRSTQTCAVGAAMAGAVVAGAAAGGHANFADAAAAMSHADDK
                     VYVPDENSSRQYNRLYALYKKLHDSFGVAGHQDALYPVMKELLAIRDEARQA"
     gene            complement(9884..9959)
                     /locus_tag="PKN92_07455"
     tRNA            complement(9884..9959)
                     /locus_tag="PKN92_07455"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9924..9926),aa:Thr,seq:cgt)
     gene            10148..10777
                     /locus_tag="PKN92_07460"
     CDS             10148..10777
                     /locus_tag="PKN92_07460"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNZ48666.1"
                     /translation="MKIVMLGAPGAGKGTQAVRMAQELGVPHISTGDIFRKNLREGTA
                     LGKEVQHYLDSGALVPDQLTCDIVADRLTESDCANGYILDGFPRSVPQAEMLTGFLKE
                     RGEAIDAAINIDVPDEEIVGRLGARRSDPETGAVYNLIFNPPPAAIADKLIQREDDKP
                     ETVQARLKTYHETTAPIIGFYEKLGVLKNVGGSKASPDEIYDRIMAVLR"
     gene            complement(10859..12391)
                     /locus_tag="PKN92_07465"
     CDS             complement(10859..12391)
                     /locus_tag="PKN92_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNZ48667.1"
                     /translation="MKSLVVNVNPLETRIALMENNRLAELIIEREEDRSIVGNIYKGR
                     VDAIIPGIQAAFIDIGFEKNGFLYVSDIAGAEGTGDIELEHGVPRASSRKKRAHQQAI
                     ETMMKKNQYVMVQVSKDKLGSKGPRLTNFITFPGRYLVLLPTVSTLGVSRKIESDKER
                     ERLRKVLRELRPKGMGIISRTACEGRTREELKEDIDYLVRAWEKTRNAYERGKKTALL
                     REDLGPILRAVRDLFKADFDRLTIDDEVEYGKIMNFLEQFAPQLKKKVRLYREKKPLF
                     EKMGLEAEIEKALRRKVYLKSGGYIAIDHTEALIAIDVNTGKFTGKKQLEDTVLQTNL
                     EAAEEIARQLRLRDMGGIIVVDFIDMEYPQNRRTLIKQFREALRNDRARITLSEVSEL
                     GMIEMTRKRVKHNLIKALSQHCPYCEGSGMVRSVTTVTFDTLRKLQDLFCRTREKHII
                     LQAHPDVARRLRGENSDLLQVIIERFGREISIESVSDFHIHEVHFLRARNRSEIETDM
                     LA"
     gene            complement(12637..14292)
                     /locus_tag="PKN92_07470"
     CDS             complement(12637..14292)
                     /locus_tag="PKN92_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48668.1"
                     /translation="MRKHILFIIVPVLAISCVLCPPDAQAWGPKAMRSITAMSLQVLK
                     NDYSDVFRPGGIVGVNFEKDVASGSADGWQILAKFTPLNSDAEVVEAVASEIQLLREA
                     RTYGPTSYFAYRMGVLSSLTAHIMMPYGFVWTAEDQEMRRKVVTDLEQQVDSFHFRVP
                     KKNRDFIRNAGTFFQEKRSSFAEDKRLIAHDYRIGKNYNGYLKQGGQAYFIRAVETVA
                     DVWNTVLQHEDTVRAFGLSRPSDRSLAWYFVQEMEYLLNVKDNMTQVEIVYKNFEKVG
                     VGMTDAMEYIGDMLYAYPQKSVKLRGVAEWQKAFDMGGKDRLHLGSKLSAHYMQEGND
                     YLAHAAQPDAEETDLNNAKRAFEDALNYDRSNEAAAKLIQETDVAIRERNERLEVVLS
                     IIATGERIHEEANRYREMQDFANAISTYRQAIGFFDAVDDEFKVHANTARENVRRLRR
                     EISDLINEVLDAASQVIDEGDRARDNNQFDEASNKYQSVSRIVSVIPEDESATVLRDK
                     QEVIDLAGRKLEESNVQKLRYEQMLQEQAQQAQAAQQAQQQRR"
     gene            complement(14487..16802)
                     /locus_tag="PKN92_07475"
     CDS             complement(14487..16802)
                     /locus_tag="PKN92_07475"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ48669.1"
                     /translation="MASSLALHLTKWILDLSSRLIKAKVRLHNAEVIDPTASIIFVAN
                     HFTRLETILLPWLIHQHTGLTPGSLADAALFQGRIGSFLKATGSISTENPDRDSLIIR
                     ALLRGDHPWIIFPEGAMIKDKKTRSQSGEFEIFDGNRKRPPHSGAAALALRAAYYQTK
                     LQCLHKREDESDLAKALRKFDIENSSDISNRKIALIPTNITYYPIRARENIFLKAARL
                     MAPDLSSRSLSELSVEGTVLSEDTDIDIRFGEPIEVLRYLEKPEYASFMSCSLNDLES
                     LESNPKQMLQDVALQITNRYMDEIYKNTTINIDHILAEIIRQQPDDRILSERGYRERA
                     YLCIHRILRSDCQVHPDLSDQCQRLIYDEPHEAFTSFMDMAVQTGFISPVKRGFKRCP
                     RYIMLTPTFHEMPRDATPEVIANEFEAVRSIPPSVRRIAFAPDFLIKAALRRHLVRLD
                     REDFEEDYARFYQPAESKPPWVGRPFLLRPWRIRGGVVLAHGYMAAPLEIRALAEHLC
                     RAGYAVYGVRLKGHGTAPEDLEQRQWEEWYASLNRGYAVMRTITNHIVVGGFSTGGCL
                     ALLAGAQKLSHIKAIFTICAPLHVRSYSIRLVPSIVTVNTLLKKLGQKQYAKEYVDNN
                     PENKHINYTRNPLTGVKELKAVMAAAAEVLLRVQQPTLVIQASKDTTVDPSSGPEIFE
                     GLGAVDKQLTLFERRRHGIINGEGSPEVFVQVEQFLHRTHKQAQSRQYWQFGRRLGRF
                     FYRRKPESENEVQEVQPSDAEVIIEEEKENY"
     gene            17074..>17557
                     /locus_tag="PKN92_07480"
     CDS             17074..>17557
                     /locus_tag="PKN92_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48670.1"
                     /translation="MRYVYLFLTLAVLSVLMNGCASLTSDLNKDRLKVESGQIYRRDS
                     SFTVRAVYEPELGAKGTALCHMVPAMARIGEAGANAVAFDLFGFSEDGKFLDPSAVET
                     VSTYALRAKDQSMVLVVRVPGQEGDSAFRSNAVRTAALALADEQLALYWIDGPDAPFL
                     A"
BASE COUNT         4192 a         4265 c         4696 g         4404 t
ORIGIN      
        1 aagtcttccg gatgagccag cagaagcaca cgggtaggaa gtccggcaag cagtgccaga
       61 cgggcaatta caaaaccgtc cccgccatta tttcctttgc cgcatacaac gaccacagga
      121 ccgtctttca catatttgaa agcggcggtg cctgcattgt tcatcaggac acaaccgggc
      181 attcccagtt cttctatgca acggcggtct gcctcccgca tttcctgtgt acttaatact
      241 ttcacagcat gccctttcaa aaaactgatc aggaacgcag tgctttggtc agctgatcaa
      301 cggtttgccg aatggtcgga ttccggtcga tttcccgatg aatcttgtcg caggcatgca
      361 gaacagtcgt gtggtctttg cctccgaact gctccccgat gtctttcaaa gaaatactgg
      421 gaatctgtgt tttgcaaagg tacatggcaa tctgccgggg gatgaccacc tgccgctgtc
      481 tgctctgacc gcgaagatct gccagtcgca cgtcaaagtg atcagccacg acacgctgca
      541 cctgttccat agtgatggga ttgatccttc cctgcccgat ctgctcccgg agcacgtttt
      601 ctgccatttc cagagagatt ttactgcctg tcaggctgca atatccgaaa agggtgatca
      661 gggcgccttc cagttcccga atgttgttgg tgatataggt tgcaatgtat cgcaaaattt
      721 ccggaggcac attcaggttt tccagttttg ccttgttctg gagaatggca atccgggtct
      781 caagatcggg cgcctgaata tctgtgacca gcccccattc aaaacgggat accaggcgct
      841 cttccatctt cttcagttct ttgggagggc ggtcgctgga aatgacgatt tgcttgtgag
      901 catcgaaaag cgtgttaaag gtatgaaaaa actcttcctg agttgcttct ttgcctgaaa
      961 tgaaatggat atcgtcgata agaagcatgt caacccggcg gtactttgcc cggaaacgct
     1021 gggtttcctt gtcggcaatg ctttgaatca tttcgttggt aaattcttca gaggaaataa
     1081 atacaacacg gaggctcggg tcaatgttca gggcgccctg accgactgcc tgcatcagat
     1141 gggttttgcc cagtccggtg cctccgtaaa gaaacagcgg gttgtaggca cggcccggcg
     1201 cctgtagaac ggcatgggca gcagcgtggg caaagcggtt tccggcgcca atcacaaagc
     1261 gttcgaaggt atatttggga ttaaaaccgt ttttaggaaa atgttttttc agcctgcttt
     1321 taccggctgg tgcgacaggc gacgttcttg ccggcatggg agccggaagg actggtttct
     1381 cctgctctgc cggttcaggg acgtattcga catgattcac ctgcggatag agtgcccgtg
     1441 ttatctccag gaccgcgtcc tgatagtgtt ctttcaacca atcggcaaag aaaccactgg
     1501 gcacacgaac ccggatgatg tcgtcttcaa gggaatcgaa actcgtttgt gaaaaccagt
     1561 ttttgtagca atgatcatcc agtgaaacct gcaagtcatt caatatgttt tgccagggat
     1621 tctgtgcagt atccgtcatg acaacccttc tattcgcttc agggaatgat cccgttcacg
     1681 gtaaattcgc attagcagag gctctttatc ttccgtcgca agttgtgtca agccttcaga
     1741 gaaggcctga tggatcgcat attcgatagt ggctgcggtc gcatgggcat catccggatc
     1801 gcccgctttc gatcatgatg ccagaccttc ggtgtctgat ccggttccaa gtttgcataa
     1861 aaccaaagcc ctatgtcaag gcagcgtaca agttattgag cgacaagctt tttggcacgc
     1921 tcccaatagc aatccagttg atcatccgtg cagtttttca gatcgcagcg ctcttcatat
     1981 gccagttgtt tcaccagagc gaagcgtttt tggaagcgat ccgaggtgcc attcaggcac
     2041 atgagagggt ctgcatccaa aaaacgggat acattcagca atgaaaaaaa caggtcgccc
     2101 aactcgttga gcgcctcttt acgctcattg tgcaccagcg ccgcctttaa ttcctgcact
     2161 tcttccacca ctttgtccag agcaccggta atatccgacc aatcaaagcc cagctctgcc
     2221 gcgtccgcct gtattttcaa ggcgaaatca atggcagaca agcagccctg ttgtaacgtt
     2281 ttcggagttt ccattttgcc ttttctttat cccttggaaa tcctattgta agcacagctt
     2341 atgtatcaga gtcaaatagg ccttttgaag cataaataca ataaaaccaa taatttcaca
     2401 aaagcacaag atggcgtata aggcagattt ttaagggcaa aatattgcgg tttccttgca
     2461 ccttcactca ttttttgata tactataagc tacgcgttca aggggtttga atgcatatcc
     2521 ttaagaggtt tcgatccatg acaaaaaatg cgtatggggc gggttctatc caggttctgg
     2581 aggggcttca agccgtccga atgcggcccg ccatgtatat tggcgataca gcgacccggg
     2641 ggcttcatca tcttgtttat gaggtggtgg acaacagcat tgacgaagcg ctcgctggat
     2701 tttgcaagaa aattacagtg accgttcata tcgataacag tgtgactgtt gaggatgacg
     2761 ggcgcggtat ccctgtggat aaccatccca atccgaagtt taagaaaaaa agcgctttgg
     2821 aagttgtgct gactgtattg catgcaggcg gcaaatttga taatacctcc tacaaagttt
     2881 ccggcggtct gcacggagtg ggcgtatcat gcgtcaatgc cctgtcgcgg tggcttgagg
     2941 tggaggtgcg gcgggacggt catatttacg ccatgtcttt tgagcgcggc gtggcaaaag
     3001 gacctataga gaccaaaggc cggacgaaat ccacgggcac gaaaatgaca tttctgcctg
     3061 acacagatgt ttttgaggtg tccgaataca acgccgagat tttgcttacg cggctgcgcg
     3121 aactggcttt tttgaataaa ggcattaaaa ttgtcttcga agatgagaga accgatgatg
     3181 aagcgattgt gatgcaatat aacggcggta ttgtcgatta tgtcaaatat ctgacgcgca
     3241 acaaggaagt gctgaaccgg acgcccattt acatggagct ggcacgtgac gaagtggaag
     3301 tggagatcgc cctgcagtac acaaccgcct acaatgaggt tgtgtccagt tttgccaaca
     3361 acatcaatac ctatgaaggc ggtacccatt taagcggttt tcgcgcggca ctgacgaaaa
     3421 gcctcaatga ctatgcgcgc aaaaacaata tgtttaaaaa agggcagaac acgatttccg
     3481 gtgatgatac gcgcgagggg ctttcagcca ttgtttctgt gcgcctgcgc agtcctcaat
     3541 ttgaggggca gacgaaaatg aaactgggga acagcgatgt acaaggtatt gtcagttcgc
     3601 tggtttatga agggctccag atttatttcg aagaaaatcc cgctgtcgca aaccggatta
     3661 tcaacaagac gctggaagcg gcgcgcgccc gggaagcggc gcgtaaagcc cgggaactta
     3721 ccaggcgcaa aggtgcgctt gacagcatcg ggcaggcggc aaaacttgcc gattgctcgg
     3781 agcgggatgc ctctcaatgc gaattattta ttgttgaggg cgacagtgcg ggcggttctg
     3841 taaaacaggg cagagatcgc cgctttcagg ctgtgctacc cctgcgcggc aaagtgctga
     3901 atgtggagcg ggcacggcag gacaagatgc tggaaaacaa agaaatacgc tccctgatta
     3961 cttccatcgg agccggcttc ggtcaggatg aatttgatct cgcgaaactg aggtaccaca
     4021 aaattattat catgacggat gctgatgtgg acggcgccca tatccgtaca cttttgctga
     4081 cttttttctt cagacatatg ccggagctga tccggaacgg acatctgttc attgcacagc
     4141 cgcctctgta tcaattaaga aaagggaaaa agatccagta tgtcaataca gaaaatgagt
     4201 accaggattt ccttttcgaa cttgccttgg acacagtcag agtgcagtcc gccgatgctc
     4261 cgcaggaaaa aaacattgat ttgaagtctc tgggaaaatc tatccgcgcc gctcaggata
     4321 gagaacgaat gatcagccgc gtcaatcgtg tatacggtgt tgaggcagag gaactgaaaa
     4381 aatgcctgct tctttcccgg gaaaaatata tttctccgca aactctcggc tatgaggacc
     4441 tggtaaccct ttttggctca aaagaagtga ttaacacctc tattgagcag ggggatctgt
     4501 tggaagagga aaacggcagg cacccgggga atggaaacgg caacggaaac ggcggacgcc
     4561 gtcctgtcat ccgacagaaa aaccaggttg atctggcatt tttcaaaagc catgagtttt
     4621 cagctttact gacaagcaac gatgcactta cagccattgg agagccgcct tatcaggtct
     4681 attctttgaa cgaaaaagcg gaagcgccgc tttttcagtc ggaagacctg ctggagttgc
     4741 gtgattttct gctggagaca gggcggcaag gacttatggt gcagcgctac aaagggctgg
     4801 gcgaaatgaa tgccgagcag ctgctcgaaa ccacgctgga tccccggaat cgtgtgctgc
     4861 tggaggtgaa agcggaagat gaggcagctg ccgacgatat gtttaccatg ctgatgggcg
     4921 atcttgtgga gccgcgtaag gacttcatcg aaaagcacgc tgctgaagta cagaatttgg
     4981 atatatagag taaatcaccg tacagcaaac tgtttgtgac gatataaaga aagcatagtt
     5041 cttccatgga tcaaaacgac agaatcaagc ctattgccat agagcaggag atgcgcagtt
     5101 catttctgga ttattccatg agcgttattg taagccgcgc actgcccgac gtgagagacg
     5161 ggctgaagcc tgtacatcgc cgcatcttgt ttgccatgca caaccttggc cttgttaaaa
     5221 accgtccctt ccggaaatgt gccgctattg tgggtgatac catgggaaaa tatcaccccc
     5281 atggcgattc atccatctac gacacgctgg tgcgtatggc gcaggactgg agcatgcgtt
     5341 acccgcttgt ggacgggcag ggcaacttcg gatccataga tggagacagt gccgccgcca
     5401 tgcgatatac agaatcgcgc atggaagcca ttacagcgct gatgcttgca gacatagaca
     5461 agcagacggt ggatatgatg agcaactatg acggacgtga acaggaaccc actgttctcc
     5521 cttccgccat acccaacctt ctggttaacg gttcctacgg gattgccgtt ggcatggcaa
     5581 cgagttgtcc gccccacaat ctgacggaag tctgtgatgc cctgattcac ctcattgaca
     5641 atcccgaagc atctccgaaa gatctcatgc gatttgtgaa gggacctgat tttccgacag
     5701 gcggcacgat ttgtggccgc cgcggtattt ttgatgccta cacaacggga agaggaaaag
     5761 ttactgtccg tgcgaaagcg gttattgaag cgcggaaaga cggttctcag gcaaaaatta
     5821 ttattcagga aattccttat caggtgaata agacccggct catcgaaagc attgcggagc
     5881 tggtgcacaa aaaaatcgtc gaaggcattt ctgacctgcg tgatgagtcg gataaggacg
     5941 gcatgcgtgt tgtcatagaa gtgaaaaaag gggaagagcc tgaagtcgtt ttgaatcagc
     6001 tgtataagca tacccagctt caggctacag ccagtattat tatgctggca ctggttaata
     6061 atgcgccccg ggtgctgaat ctccgggata tgctgttttt ttatcttgag caccgcgctg
     6121 aaattatcac acgccggtca cgctttgaac tcgcaaaggc ggaggcacgc gcccatattg
     6181 ttgaaggact tctgaaggca agcgatcata tcgatgaagt gataaaaatc atccgcgcgt
     6241 ctcaggatga tgaaattgcc cgacagcggc ttatggatcg gttcgagttc agcatacccc
     6301 aggcaaacgc cattcttgcc atgcgtctgc gcaatctcac cggactgaaa caggcggagc
     6361 tggaagagga ataccgggat ctcctaaagg aaattgagcg gctccgctat attctttcca
     6421 gcgaaaaaac cattatggca gaagtgcgga aggaagtgct ggaggtaaag gaaaaatatg
     6481 gagatggccg ccggacagag attctgggcg atgtgggaga atttaagatc gaagacctga
     6541 tcgccgatga aaatatggtc gttacttttt ccaatgaggg ctatataaag cggctccccg
     6601 ttgcctccta cagaaaacag cgccgcggag gaaaaggcat taccgggatg ggaaccaagg
     6661 aagatgactt cgtgcgcgat ctttttatcg cttccgccca tcagtatatc ctttttttca
     6721 ccagcctggg acgtgtccac tggcgaaaag tgcatgaact gccgcaggtc ggcagaacgg
     6781 caaaaggacg gaatattgtc aatctgctgc aactggagaa agacgaagtt gtaaccgctt
     6841 atctgcccgt tcggaacctc aaagaagaag gtcactatgt tttcatggtg acccgtcagg
     6901 gtgttgtgaa aaaaacgccg ttggttcagt tcagtaatgt gagaaccggc ggaattattg
     6961 ccctgactct gaaagaaggg gatgtcctgc tggataccct gatcaccacg ggtgatgaca
     7021 agatactgat tgcgaccaat cagggtaaag caattcgttt cctcgaaagc gatgtgcggc
     7081 ctatggggcg cacggcagcc ggtgttatcg gcatgaagct gcagcctgat gactttgtgg
     7141 tcggtgtgtc cctcgccaaa gatgaccgca ccgtgttaag tgtcactgaa aatgggtatg
     7201 gaaagcgtac caaggtcggt gaatacaggc ttcagcatcg gggcggacag gggactattg
     7261 atatcaagac cggggagcga aacggcaatg tggtcgccat gctcaccgtt gatgatgatg
     7321 acgatctggt gctgatcagc acagacggga cagtgatccg cacaggtgtt tcgggaatac
     7381 gcaccattgg ccgcaacaca ctcggcgtga agattatgaa tccacagccc ggtgctgtgg
     7441 taacggcggt ggcgcgggct attggcgata aaaaagagga agatgctaca gagtccgcat
     7501 cggatgagac caccgaagct ccggaagacg agggtgtcgg ctacatcgaa cagatggact
     7561 ttgaagagga tctggaagac agcgttgaag aagatacaga agaataaaat ggcgccccga
     7621 acaggaatcg aacctgtgtt tagcgcttag gaggcgctcg ttctatccac tgaactatcg
     7681 gggcgcaacg cactgccgcc ggcgtacata ttgtatgcgg gcggcttttt ttatttgagg
     7741 cgggattact ttgggcaata ggttacacca gaaaggcttc aaatcccaaa ttgctgcctt
     7801 ttttagtgtc gtaactcgtt agaaatgtcc ccttccttaa ctcttttaaa ctgtcccctt
     7861 gttttttgtt caatacgtga tggcagggct atgctgaaga gcaacccggg cacttgaaga
     7921 gcaggcaaaa ggcgtggcag cgttatgcct tattgccgga tattagacag cacgtttcag
     7981 ggatttgcag gaatggtcca gtgtattgct ttgcagggct ttgcatttcg ggctcttctt
     8041 acagcaggtg ccttttgcat ttaaggacgg cgagattaag cccgtttgtt tgcagtttct
     8101 tcccttaact gggctataat caggggagtg ttgtccagtg gactgtctca gcaaccctaa
     8161 gaaagagaac tgtcatgaaa gaaaagtatg ttcttggtct tgattatggc accaactctg
     8221 cccgtgccgt cgttgtcagt gcaaagacag gagcggaaat cggcaccgct gtttttgaat
     8281 acccttccgg cgagaaaggg attattctag atgaaaagaa tcctgatctt gcgcggcagc
     8341 atccctggga ttatcttcag gcgctggcag cgatcgttcc tgccgcactg aaagaggctg
     8401 aatcggtgcc cggcttttcg gtggacgata ttattggaat cggtcttgat gcaacaggct
     8461 ccacgccttt gccggtggat aaaaatggca cgcctctggc gcttctcgat gatttttcca
     8521 aaaatcctgc ggcaatggcg tggctctgga aagaccacac gggtcacgcc gaagcagtgg
     8581 agattacagc gcttgccgaa aaaatgcggc cccaatatct tgccaagtgc ggaggtgcct
     8641 attcgtccga atggttctgg agcaaaattc tttattgtaa gcgctccgcc ccggatgtgt
     8701 tttatgccgc ccacacctgg gtagaacatg cggactggat gtcagctgtg cttacgggga
     8761 ccgcccatcc tgagagcctg aagcgcagtg tctgcgctgc cgggcacaaa gcaatgtacc
     8821 atcagagctg gggtggctat ccggatcagg aatttctgac ctcgcttgac ccggcgctgg
     8881 ggcgcattcg ggaaacactt ccgaaccgcg cctattccat tgccgaaaaa gcaggcggtc
     8941 tgactgaaga atgggcgttg aaactgggcc tgccgacggg tattcctgtt gccatgggcg
     9001 ctatcgatgc acatatgggc gctgtcggct caggaattca gcccggagtc ctggtaaaaa
     9061 tacttggcac ttcctgctgt gacatgctgg tagcgcccct tgagcaggaa cttccggaca
     9121 ttccagggct ttgcggtatt gttcctgaat caatcctgcc cggctgttac gggctggaag
     9181 cagggcaatc agcggtaggc gatattttta actggtttgt aaataatatc cgccccggag
     9241 gggaagggca tgaaagtctg acggaaaaag cactgaaact gaagccggga gaaagcgggc
     9301 ttcttgccct tgactggcac aacggaaacc ggacagttct ggtagatcag cgtctgacag
     9361 gcgctttgct ggggatgacg ctgcacacgc gccccgaaga aatctatcgt gcgctcattg
     9421 aggcaactgc cttcggtgcc cgggtgatta tggagcggtt tctggagtat ggcatgaccg
     9481 tggatcgcat tatcaactgc ggtggcattt ccggtaaaaa tgcgctgctg atgcagattt
     9541 atgctgatat tatgggcaga cccctggagg tgtcacgaag cactcaaacc tgtgctgtcg
     9601 gcgctgccat ggcaggagcc gtagttgccg gagctgccgc aggcgggcat gctaattttg
     9661 ccgatgccgc tgccgccatg tcgcacgccg atgataaagt atatgtgcct gatgaaaaca
     9721 gcagccgcca atataacagg ctttatgctt tatataagaa actgcacgac agcttcggcg
     9781 tggcagggca tcaggacgca ctgtatcctg tcatgaagga actgcttgcg atcagggacg
     9841 aagccagaca ggcgtgaacc agggcgtaaa ggagagaaaa ggatggagct ggaacgggga
     9901 atcgaacccc ggacctcgtc attacgagtg acgcgctcta cctactgagc tattccagcg
     9961 ccggcgaaac atggaggtac accagacagg ggtgcttggt gttgctacat tttacccaag
    10021 cagccacgtc aatatcaact tcgttatgca tttccgctgt gttatactct ctgcggctgt
    10081 aatgcagccg cagacgggcg gagaacagcc tcaccaaatt acaacaacag gagaagcgat
    10141 tatgggattg aaaattgtta tgcttggtgc cccaggtgcc ggaaagggga cacaggcggt
    10201 gcggatggcg caggaactgg gcgttcctca catttccacc ggtgatatat tcagaaagaa
    10261 cctgcgggaa ggcacagcgc ttggaaaaga agtgcagcac tatctggaca gcggcgcttt
    10321 ggtgcccgac cagttgacct gcgatattgt ggcagatcgt ctgactgagt cggactgcgc
    10381 caatggctat attcttgacg gtttcccgcg ttccgtgcca caggcggaaa tgctgaccgg
    10441 ctttctgaaa gagcggggcg aagccattga tgctgccatt aatattgacg tgcctgatga
    10501 ggaaattgtg gggcgtctgg gtgcccgcag gagcgatcct gaaacaggtg cggtgtacaa
    10561 tcttattttt aatccgcctc ccgcagcgat tgcggacaaa ctgattcagc gggaagatga
    10621 caagcctgaa acagtgcagg cgcggctgaa aacctaccac gaaaccacgg cgcccattat
    10681 cggtttttat gagaagctgg gtgtgctgaa gaatgttggt ggcagcaaag ccagtcctga
    10741 tgaaatttat gacagaataa tggctgttct caggtaagca tttgctgagc atgcagctct
    10801 tttattggcg cccttttccg gctcaggcgc atcctgccgg agaagggcgt ttttttggtc
    10861 aggcgagcat atccgtttca atttcgctgc gattgcgcgc ccggagaaaa tgtacttcgt
    10921 ggatgtggaa atctgaaaca gattcgatgg atatttctct gccgaagcgc tcaatgatga
    10981 cctgcagcag gtcgctgttt tctccgcgca ggcgacgggc tacatccgga tgtgcctgca
    11041 ggataatatg cttttcccgg gtgcggcaaa agaggtcctg cagtttgcgc agagtgtcaa
    11101 aggtcaccgt ggtgacggag cgcaccatgc cgctgccttc acagtagggg caatgctgtg
    11161 acagtgcctt aatcagattg tgcttgaccc gtttccgggt catttcgatc atccccagtt
    11221 cgctgacctc ggagagagta attcttgccc ggtcattgcg cagcgcttct ctgaattgtt
    11281 tgatgagagt gcgcctgttc tgagggtact ccatatcgat gaaatcgacc acaatgatgc
    11341 cgcccatatc gcggagccgc agctgacgtg cgatttcttc ggcggcttca agatttgtct
    11401 ggagcacggt gtcttccagc tgttttttgc cggtaaactt tcctgtgttt acgtcaatgg
    11461 caataagcgc ctccgtgtga tcgatggcta tatagccgcc gcttttcaga tagactttcc
    11521 ggcgcagtgc tttttcaatt tccgcttcaa ggcccatctt ttcaaacagt ggttttttct
    11581 ccctgtaaag ccggactttc tttttgagct ggggcgcgaa ctgctcaaga aagttcatta
    11641 ttttgccgta ctcaacttca tcgtcgatgg tcaaacggtc aaagtccgct ttaaagagat
    11701 cacgcacagc ccggaggatc ggtcccagat cttcccgcag cagcgctgtt tttttgccgc
    11761 gttcgtaagc gttgcgtgtt ttttcccagg cacgtacgag ataatcgata tcttctttca
    11821 gctcttcccg ggttcgcccc tcgcaggcgg tgcgcgaaat gattcccatc cctttgggtc
    11881 gaagttcacg cagtaccttg cgcaggcgtt cccgttcttt gtccgattca attttccgtg
    11941 agactccgag tgtgctgaca gtgggaagga gcacaagata tctgccggga aaggtgatga
    12001 agtttgtaag gcgtggccct ttggaaccga gtttgtcttt tgacacctgc accatgacat
    12061 attggttctt tttcatcatg gtttcaattg cctgctggtg cgcccgtttt ttgcgtgatg
    12121 aggcgcgggg cacaccatgc tccagctcaa tgtcgcctgt tccctccgcc ccggcaatgt
    12181 cggaaacata aaggaagccg tttttctcga atccaatatc aataaaagca gcctgaatgc
    12241 cggggataat agcgtccaca cgccctttat agatatttcc gacaatactt ctgtcttctt
    12301 cccgttcaat aatcaactcc gccagccgat tgttttccat cagtgcaatg cgggtttcca
    12361 gcggatttac atttacaaca agacttttca ttgattctcc tgatgatatt gcgcggcatg
    12421 tcaactgcta ttcggcaggt actgcatttg aaaaatacgt gcagtccgta cttctgacta
    12481 cgcatcatct actgatgctc tgccggggac aataaagtgc ctcgcaaaaa aacgtgttgc
    12541 agtttctgaa gatcacatcc gaaactgttt tcttttaaaa aaaacgccac ccccttgtat
    12601 ggaggtgacg ccttaaatga tctgtccgga aaaaggtcag cggcgctgct gctgtgcctg
    12661 ctgtgccgct tgtgcctgct gagcctgctc ctgaagcatc tgttcatagc gcagcttctg
    12721 tacgttggac tcttcaagtt tcctgccggc aaggtcgatg acctcctgtt tgtctctcag
    12781 cactgttgcc gactcgtctt cgggaatcac cgagacgatg cgggaaacgg actgatattt
    12841 attggaggct tcatcgaact gattattatc ccgcgcccgg tcgccttcat caataacctg
    12901 acttgccgcg tccagaactt cgttgatcag atctgaaatt tcacggcgca gacggcgcac
    12961 attttcacgg gcggtattgg cgtgtacttt gaattcatcg tctacagcgt cgaaaaagcc
    13021 gatagcctgg cggtaggtgc tgatggcatt ggcgaagtcc tgcatctctc tgtagcggtt
    13081 ggcttcttcg tgaatacgtt cccccgtggc aatgatgctg agcaccactt cgagccgttc
    13141 attgcgctcg cggatggcaa cgtccgtttc ctgaataagt ttggcggccg cttcattgga
    13201 acggtcataa ttcagtgcat cctcaaaggc gcgcttggca ttgttcagat cggtttcctc
    13261 agcgtcgggc tgtgccgcat gcgccagata atcgttgcct tcctgcatgt agtgggcaga
    13321 cagtttggag cccaggtgca gacggtcttt tccgcccatg tcaaaggctt tctgccattc
    13381 ggcgacaccg cgcagtttga cgcttttctg gggataggca tagagcatat cgccgatata
    13441 ttccatggca tcagtcatac ccacgcccac tttttcaaag tttttgtaga caatttcaac
    13501 ctgagtcata ttgtctttga cgttgagcag atattccatt tcctgcacaa agtaccaggc
    13561 aaggcttctg tcagaaggac gtgataaccc gaaggcacgg acggtatcct catgctgcag
    13621 aacagtgttc cacacatctg ccactgtctc aacagcgcga ataaaatagg cctgtccgcc
    13681 ctgtttcaga tatccattat aatttttgcc aatcctgtaa tcatgggcga tcagtcgttt
    13741 gtcttcagca aatgaagagc gtttctcctg aaagaatgtc cctgcattgc ggatgaagtc
    13801 tctgtttttc ttgggaactc tgaaatgaaa agaatcgact tgctgttcca gatcggtcac
    13861 tactttgcgc cgcatctcct gatcttctgc tgtccacacg aatccgtagg gcatcataat
    13921 atgtgcagtg agagaactga gtactcccat tctataagca aaataggacg tgggaccgta
    13981 ggtgcgtgct tcacggagca gctgaatctc gctggcaacc gcctccacca cttcggcgtc
    14041 tgaattcagt ggtgtaaatt tggcgagaat ctgccagcca tcggctgatc cgcttgcgac
    14101 gtctttttca aaatttacgc ccacgatacc gcccggacgg aagacatcgg agtaatcgtt
    14161 tttaagaacc tgcaggctca tggcagtaat actgcgcatg gctttaggtc cccaggcctg
    14221 tgcatccgga ggacacagca cacaagaaat agccaacacc ggcacaataa tgaagagaat
    14281 atgtttccgc atgacgctga ttcctgtggc gcatttgcag gcgcccatac tgttttaggt
    14341 tgcgtgtccc gaaaatgagt tcgaaaccgt tacaaaactg ttagcggtac ctatgttgtg
    14401 gccgttattt taacaacaaa gaggcacgac agtctaaaca gccataatcc attaccctaa
    14461 aaactaacat aaaccatggt aaagtgtcaa tagttttcct tttcttcttc tattattact
    14521 tccgcatcgg atggctgcac ttcctgaact tcattttcag actccggttt ccggcgataa
    14581 aaaaatcttc ccagacgcct gccgaattgc cagtactgcc gagactgcgc ctgtttatgg
    14641 gtacggtgca ggaattgttc cacctgtaca aatacttccg gacttccttc accatttatt
    14701 ataccatgac ggcgccgttc aaagagtgtc agctgtttgt cgactgcacc cagaccctca
    14761 aatatttccg gaccgctgga cggatcaacg gtagtgtcct tggaagcctg tatgacaagc
    14821 gtgggttgct gcacgcgcag caggacttcg gcggcagccg ccataaccgc tttgagttcc
    14881 ttaactccag tcaaggggtt gcgtgtgtaa ttgatatgtt tgttttccgg attattgtcc
    14941 acatactcct tggcatactg cttctgtccg agttttttaa gcagtgtatt tacggtgacg
    15001 atggagggca ccagccggat ggagtagctg cggacatgca ggggtgcaca aatcgtgaat
    15061 attgctttga tatgtgaaag cttttgtgcc cccgccagca gtgccagaca accgcccgtc
    15121 gaaaatccgc ctaccacgat atggttggtt atcgttcgca ttacagcata gccacggttc
    15181 agtgatgcat accattcttc ccactgcctt tgctccagat cctcgggtgc tgtgccatgc
    15241 cctttgaggc gtacaccgta gacggcataa ccggcgcggc acagatgttc tgcaagggca
    15301 cggatttcca acggtgccgc catgtagccg tgcgcaagca ccactccgcc ccggatgcgc
    15361 caggggcgca gcagaaaggg acgccctacc cagggaggtt tgctttccgc cggttggtaa
    15421 aaccgggcat agtcttcttc gaagtcttcc cggtccaggc ggacaagatg gcgacgtaat
    15481 gcggctttta tcagaaaatc cggtgcgaaa gcaatgcggc gaaccgaagg gggaatgctc
    15541 cttacggcct caaattcgtt ggcgataact tcaggagtcg catcgcgggg catttcatga
    15601 aaagtgggcg taagcatgat atagcggggg cagcgtttaa atcctctttt taccggcgat
    15661 ataaaaccgg tttgcaccgc catgtccata aaggaggtga atgcctcgtg aggttcgtca
    15721 taaatgaggc gctgacattg atcgctcaga tcgggatgca cttgacagtc ggatcggagg
    15781 atccggtgta tacagagata ggcacgctct ctgtagccgc gctcgcttaa aatccggtcg
    15841 tcgggctgct gccgtatgat ctcagccaga atatggtcga tgttgatcgt agtatttttg
    15901 tatatctcat ccatgtacct gttggtaatc tggagagcca catcctgaag catctgtttg
    15961 ggatttgact ccagactttc cagatcattc aggctgcacg acataaaaga ggcatactcc
    16021 ggtttttcca gatagcgaag cacttctata ggttcgccaa agcgaatgtc gatgtccgta
    16081 tcctcggaaa gaacggtgcc ttcaacagat aactcgctga gagagcggct gctcaggtca
    16141 ggcgccatca gccgcgctgc tttcagaaaa atattttcac gggcgcggat ggggtaatag
    16201 gtgatgttgg ttggtatgag ggctattttc cggttggaaa tgtcggatga gttttctata
    16261 tcgaatttcc ggagcgcttt tgccaggtcg ctttcgtcct ccctcttgtg gaggcattgc
    16321 agtttggtct ggtaataggc ggcgcgaagt gccagtgcgg cggctccgct gtggggtgga
    16381 cgttttctgt ttccgtcgaa aatttcaaat tcacccgact ggctccgcgt ctttttgtcc
    16441 ttgatcatgg caccttccgg gaaaataatc cacgggtgat ctccacgcag gagcgcgcga
    16501 atgatgaggc tgtcccggtc ggggttttct gttgaaatgg atcctgttgc cttcaaaaaa
    16561 gagccgattc tcccttgaaa aagcgctgca tcggcgagag agcccggggt cagcccggta
    16621 tgctgatgga tgagccaggg aagcagaatc gtttcaagac gcgtgaagtg gttggcaaca
    16681 aagatgatgc tcgcagtcgg atcaatgact tcagcattgt gcagccgtac ctttgccttg
    16741 atcagcctgc tggacagatc cagtatccat ttggtcagat gcaatgccag agaggaagcc
    16801 ataaaaagca aaaatccttg ctgttttttt ttacactgtc cggagttctg ttgtctgtcg
    16861 atgtgccact atgggatctt atcaacagta taataatctt cttcctctaa aaagtccacc
    16921 gtgcaggagt ttttgtatcc ggatatttat gaaaagcgac ctgcagcctc ctgataatta
    16981 aaccttttat tggctgccgg attttgctat agttagattt tacaggttgt tctttgcaac
    17041 gccttcgctg gagaaggcgg aagggtttgt tttatgcgat atgtttacct ttttctgact
    17101 ctggctgttc tttctgtgct gatgaacggg tgcgcctcac ttacttcgga tttgaataag
    17161 gatcgcctga aagtggagtc cgggcagatt tatcggcgtg attccagttt tacagtgcgt
    17221 gccgtatatg agcctgagct gggtgcaaaa ggaacagcac tctgccatat ggttcctgcc
    17281 atggcgcgga taggggaagc cggcgccaat gccgttgctt ttgatctttt cggcttttct
    17341 gaagacggaa aatttctgga tccttcggcg gtagagactg tttccaccta tgcactgcgt
    17401 gccaaagacc agagtatggt gctggtggtg cgtgtgcccg gccaggaggg agacagcgca
    17461 ttccgaagca atgcggtgcg cacggcagcg ctggcgctgg ctgacgagca actggcttta
    17521 tactggatag acggaccgga tgcacctttt cttgccc
//
LOCUS       DAOPWB010000065        17503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3297_length_17503_cov_6.554448, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000065 DAOPWB010000000
VERSION     DAOPWB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17503
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3297_length_17503_cov_6.554448"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1158
                     /locus_tag="PKN92_07485"
     CDS             298..1158
                     /locus_tag="PKN92_07485"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNZ48671.1"
                     /translation="MYPTLLSWGELRFHSYTVMMTLAFLIGALGPNWLNMRRKEPYAA
                     TPAGGIWIFIGGIAGAKIYWVIQYGGWDDLRYLQFFLTGGLVFFGGLIGGIIAGIAYL
                     KYAKTPVIPAADMVAPFLAFAHGIGRIGCFLNGCCWGRLCSAGLLWGVSFPRASYGPF
                     REQVRKGLIDGAADFSLPIHPVQLYETLGLFVLFVVLMIIYKKHKHTGVVSLSYVTLY
                     GLLRLTTEGFRGESVRSVFGWLTVSQTIGLAMFVTGGLLFLLFKCTIWKDRKSDDASE
                     VTVVLKESDL"
     gene            1239..2948
                     /gene="polX"
                     /locus_tag="PKN92_07490"
     CDS             1239..2948
                     /gene="polX"
                     /locus_tag="PKN92_07490"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNZ48672.1"
                     /translation="MENFQISRLLDETAFWLEIAGESPFAVRAYATAATTVNRSDLEI
                     AELVLSHQPYSLPGIGKTLSSQIEECVLHGRITFLDDLRKRFPESLRSLLKVPGIGAK
                     LIRLFYEQLGITTLPLLKENCLNGEIQKLPGFGEKRVQKLLEAIAFAEAQQGKYRITA
                     IEDEARALSSFLSALPCVLRFKITGKLRRFSNVYEKLVFVVLGSDIGQIKAALEETFA
                     GISQSGCVPEKFSFRTAPGMLCEVYVTEDQACWVPLLIETTGSAQHVAALKQLSASKG
                     LLWQSSVLKDAQGHPFNFEKEEAFYALLGIPYCEPVIRENVYLPVVSDTPELLNLGDL
                     KGLVHCHSQWSDGAHSLESMALACRDLGYTYMLITDHSQSSKIANGLEPDRIAAQHQE
                     IDALNRRLTGIRVLKGIESDIRKDGSLDYDEAILRTFDWVIASVHNGLDLDKTEATRR
                     LIRAMENPFTSAIGHPSGRHLLTRPGYELDMEKITDAAVTNKVALEINASPKRFDLDW
                     RHLKDAAHKGARFIISPDAHRITGFQNVRYGVQLARKGCLSSDHILNCLPPEEFMAWQ
                     KKP"
     gene            2930..3607
                     /gene="nth"
                     /locus_tag="PKN92_07495"
     CDS             2930..3607
                     /gene="nth"
                     /locus_tag="PKN92_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNZ48673.1"
                     /translation="MAKKTINAAEEKKRVAAIYRLLQETYPEAACTLNFRSPLQLLIM
                     TILAAQCTDARVNIVCTQLFKDFQSVDDFADAAPGALEKAIHACGFFNQKARAIRESC
                     RMLRAEYNSQVPDTMDDLLRLPGVGRKIANAVLGECFGKPAVVVDTHCKRVANRLGFT
                     RHQDPGKIEIDLQQLWQPEHWTMYSQYMVHHGRAYCLARSPKCADCPLFSLCPWPEKD
                     KRAHGAF"
     gene            3594..4349
                     /locus_tag="PKN92_07500"
     CDS             3594..4349
                     /locus_tag="PKN92_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48674.1"
                     /translation="MAHSDPSKAAVLPVAEGRTSFAWISILYVGLVFTIDTLASQRVS
                     FLFDWTMFSWSLSDLLEYLPDFQKGSPLLRIAFHFDLFKFLAWFLFPFVLCWKNLDRQ
                     WLLPTHTQKRPRLFYFFSILSVFVVLIALFLIPSLRDYYGGNTGAQQKSWLEAGLSFF
                     VWEMSWFPGWEFLCRYVLLRAALRIRPSWGWLVVPLFEGLYHLQKPFAEAMGMAVFSL
                     FMTAWTVQHKSMFIPFCVHFLIEIVLLIFLFLI"
     gene            complement(4368..7145)
                     /locus_tag="PKN92_07505"
     CDS             complement(4368..7145)
                     /locus_tag="PKN92_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNZ48675.1"
                     /translation="MRAQSVKLALSIPAMIRGKISVGDAVLEGVKLVYFKGSSSQQPK
                     PQKKPFLLPPDLLERLPFQRLSGSDCSLEIRQDDKSILSFDSISFSLTNDSGKGALLV
                     ALETAFSVGASAGKIKVAGDLQLPGHVRGEVHFDGINGALLRHFTPVPDGLDGALEGN
                     VSFSGDTEKSLLVETTLDISRFTFPDLPAAFSSINGALRSVIQWSSADKQLRVLSGDL
                     STDLLNLKGQGVIDFSADSPQVQASVTGENLPLSAILAEYLPKTLNRLGKPVIHLSGN
                     EYIVCQVTGALEHPSIKVEAAVPEITLALQPSNKEIPEVAFRLQQIRASWDSVSAVPE
                     LSALVSDGSIKAAKAGFEATQVSGVISLHGKTISLQPFSALLAEKDLYGSATYDLEKE
                     KAAFKITGELSALENTILKDSIKNLKLAGDIAFHASGTYLAGGPLTVKASADVTRGMV
                     AFEWWLKKPVGVGATIKSLNAEILPGKSMTITGEAFVEDTKILADLKYRYSDDKKWEN
                     TRTRVDLPHLEVNSAGKCVDIPYTALGGACKNGFYESNYVGPLPGDSIAKLGGHFDHV
                     SFFPDGGIHPLDCTDTSVEVTITTIKNKPKTTEFVIHAGDAKVPPFSEKWILPMGAKE
                     EEQDSEEKPSEPTFWIFNLSADSISLPPWQGKDFSGQLHDNPDETYFSFFRGKMGDGQ
                     VNGAYHHEKRDNIMTLKGNWEEIPAVYLIRHLELPEILEGNCTGTVSYTVDQDDPGNT
                     LETEGSFSVTNGHFIEHKLYEVFTSSFAQKFGVLPPAALRFKKLSSDLKIKGDQIFTD
                     NVSIESDGMTITGHGIWILEGDMNYRIDLSMSPDLAEQISIMRESFNIQGFRMTQRNI
                     ELGFQLSGPTFNPTSELAGLPPIGVTFVSGAADLTGEAMKLLDTPRQMFMSIFRSGGG
                     ILGATRTQTQQ"
     gene            complement(7413..8459)
                     /gene="recA"
                     /locus_tag="PKN92_07510"
     CDS             complement(7413..8459)
                     /gene="recA"
                     /locus_tag="PKN92_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019895951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNZ48676.1"
                     /translation="MAAATNDDKGKARALDIAVSQLEKQFGRGTLVRLGDDSFREGVQ
                     VISTGSLSLDIATGVGGFPRGRVIEIFGPESSGKTTLALHSVASAQRAGGIACYIDAE
                     HALDPSFAEKIGVDINNLLVSQPDTAEQALEICESLVRSNAVDIIVVDSVAALVPKAE
                     VEGDMGDSHVGLQARLMSQALRKLTAIISRSKTLLIFINQIREKIGVMFGSPETTTGG
                     RALKFYSSMRLDIRRISGLKDREENVGNRVRVKVVKNKVSAPFRQAEFDILFDEGISR
                     EGDILDLALEEKLILKSGAWFSHDGENLGQGRENTRQYLRDNPALTEKLVSQILANKG
                     LPWIAKETDEQPAE"
     gene            8591..9967
                     /locus_tag="PKN92_07515"
     CDS             8591..9967
                     /locus_tag="PKN92_07515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ48677.1"
                     /translation="MKSYGIINFSVLGGGGEVGANCFQLACNGTRILLDCGTHPKKEG
                     LACLPDFRLLEKPPDLCLLSHAHMDHCGALPYLQKCYPGVCSYSTIPTRAIADRMMRS
                     SVTVMEMLARERQIYDYPLYDHSDVSLAVRLLNGVPYNIPVEIGNGTPIELRFINAGH
                     VLGGASILLKFPGHTVFYTGDISHRRQFLIGSRERLDETERVDTLIVESTLGATEEPE
                     SSSYQSEADRLAEAIRDVLRQGGSALLPCFALGRTQEMINVICHLQRNNKLPRVPIYS
                     IGLGRAIYQIYDRYSDYLHPESSLTPLDHTVQLKNVWEKGLVQKLLLKPSIIIATSGM
                     MTENTPSALLAQSMVTQSHHGIFFVGYLDPETLGYRLLHCKPGEALQFSFSAPPVEVI
                     LENIRQFRFSAHASRRYLLQMIERLNPKNVVYVHGDPDALVWMESNSTNKALSFIPET
                     GHEFVLEA"
     gene            9970..10938
                     /locus_tag="PKN92_07520"
     CDS             9970..10938
                     /locus_tag="PKN92_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ48678.1"
                     /translation="MAKKSSPFSIFFYGVVLFFFQLTRLLPLSLSRKFGIFLGDLAFF
                     IIPRLKKIGMANLAIAYGDEMPLKEKKRILRGAARNLGIIAAEFSRIPSIAKGGLKNM
                     ISVEGIEEALSHKPAVLLAAHHSNWEWMLPVAYDLGLRTAVIVNPFRNAFLDHFVNQY
                     RLSGGTITVRKQGAVQEMENLFKNGVSPSMLGDQSPRNGGVPIKFFGSPAWASIGPAM
                     IAMKTDSPVIPASMARDNKGHYTLKFFPAIHMRNSGQYLEDLVFNTQRCQDALETIIR
                     AHPEQWLWFHQRWRKRERLEKEWAFRLEKPSQDSDTVQSLKDQESA"
     gene            10938..11654
                     /locus_tag="PKN92_07525"
     CDS             10938..11654
                     /locus_tag="PKN92_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HNZ48679.1"
                     /translation="MSLFTAYASKYSGLPYLAVAVTDRVSPDSVAPYKESRFPLCIEL
                     RVDLCEKQDPESVKNTLRSFAAFPRLLTIRSSSEGGNWQESDAVRHSLYSETLSDAEG
                     IDIELSARSICAPLIRECKALNKTVIGSFHDFSATPSIEKLDALVLEGKSLGVDAVKV
                     AAHCHSSDDLRRLTCFLLSHPQDALIVMGMGRDGTPSRLFFPFLGSLLTYTFVGTPSA
                     PGQKNLDEMLHFLNYLLPEF"
     gene            11733..12443
                     /locus_tag="PKN92_07530"
     CDS             11733..12443
                     /locus_tag="PKN92_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48680.1"
                     /translation="MKNILLPLVLLCILVGFVVACAPEPPPPPPPPEDLPPPEPSPEE
                     YRQQVRSILSSYLMTGTSAPDDAVLPEILNQTQARRAQLIATENGRQALHLVTADVDE
                     SLKLAREEERWRKISGLCRIYKALQPDSNRYEKLQSYSDEMVKRPILTVTGFIELDDE
                     LYVFIDLFDPTTGQTKAYRCREGEEFHERDNKTVMRLVRIIGNKQSIEVEYLPANYSW
                     LCIGPKERSRLGPDRKAD"
     gene            12492..14216
                     /locus_tag="PKN92_07535"
     CDS             12492..14216
                     /locus_tag="PKN92_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48681.1"
                     /translation="MPKSLKLPPSVIYIVILVALAGTLVYINTFHADWVWDDVSSVLL
                     HKHVQTPSSVFQLFLEDQHAFGRGQGNFYRPLVSLSFLIDYQLAGGSTPIEGQSSFVP
                     QISPFVFHLTNLAWHLAAALCLLGLLAALNAPYFVLLAVPLIYVLHPLHTEAVAYISG
                     RADMMSATGIFGALWSSVCYLKSAGRRRYLFFMILCFTGALLSKESSLIFPFLLTLLL
                     LTTHREPLSESLSDSSKKHCKLHLFSASFIILLVYILLRSTVLRFATESSTASAAFSE
                     RAFETFQSLGIYLKLLFAPFHLHMERTLTHAGSLHFLAGCLFFVALLGAIACSFAKKQ
                     SFIGIGALWFLISWMPISGLFPLNAPMAEHWMYVPMAGFWLCLAAILKPIVSHPQAKF
                     LLYGILGLFCLFLGTLTIQRNLDWSDNERLFLATLRENPESARVHYNLAVTYSDLVGN
                     DAAAKRHYEIFLNLRSKERAQHPDKAGLIIDDEIDARLALGKSLMNLSAYTEALGIYT
                     PLIALAEFEVWRPTAALAALRSGLCMLALGEIAQSNAYFQQAMTLEPLYAPDVERIIR
                     GENSFHIY"
     gene            14232..15626
                     /locus_tag="PKN92_07540"
     CDS             14232..15626
                     /locus_tag="PKN92_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNZ48682.1"
                     /translation="MAERLHFSGIGGTAMVAGARIALEAGCEVRGSDGVLYPPASHMV
                     QALQIPVYSGYSADNLSWSPDTVIIGNALSRGNPEVEYVLSHRLTYTSLPEWLKRNVL
                     HARHSIVISGTHGKTTTTALTAHVLKAHDPGTGYLIGGEALGFSHSSHLGKEGAPFVI
                     EGDEYDTAFFDKRAKFLHYLPNTAVVTSLEFDHGDIYHDLGDIEIAFQRMFRQIPAEG
                     LVLLCADNHALSLATHSFSCVETYGFHENALWRGNYTAEASNCQEMQVFYKQTLWGSV
                     PLPLSGQHNHQNILAAIAVAASLGMKQQDIAAAIASFKGVRRRLETFLESQGILFVDD
                     FAHHPTAIKGSIAAAKTKWPKRRVIAVVEPRSNTMVTNRFQDDFRDALKEADVVFLGP
                     IHRISSLPESDRLNREEIVQSLTAWGREAVFFDEVPELVHALHNTARQNDVVLIMSNG
                     AFGGIYTLLKEMYL"
     gene            complement(15661..16656)
                     /locus_tag="PKN92_07545"
     CDS             complement(15661..16656)
                     /locus_tag="PKN92_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48683.1"
                     /translation="MAEQIDKRPPRPLGINKGLENPGLEYTFESFRHLRTIDLLRSAR
                     EGVEVARMESSLGKDPLEIEQQVLNNVTIALEYLPLLIKDQRDIWEILTIIQNQKEDP
                     VFRKFLMEEVFGSAKPESLLSLTLPELLFSLERRKTKGVTGEVSAVAAGVIKDADEMF
                     HETMLRLASHPNEMPDIQASAIKLYYTFLDTQYLKIFDALTEVTEARKNGASVDILSV
                     KNGEIPLSRESRDALRRLTKHVEALAAGISGHVSEESVRDDTVKESAEAVFSQMQEKY
                     VGMDEAKLALLKEGLVPETDNFPTAPPMEGLPDLIKDSLIPPEGKPLSPELFMTN"
     gene            complement(16855..>17503)
                     /locus_tag="PKN92_07550"
     CDS             complement(16855..>17503)
                     /locus_tag="PKN92_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNZ48684.1"
                     /translation="TESIVTLEDPVEYQLPGINQVQIDPDINLTFASTLRAALRQDID
                     VLLVGEIRDPETAQIAIRAAMTGHLVFSTLHTNDAIEAISTLRNMGVPPFLIAAALTA
                     VIGQRLVRKMCSHCRKPFKPEIGLLTSLGLPANTKKLYRSTGCPICHFTGHSGRTGVF
                     EVLEITPEIREEIINEASADKISKMAGYQTLASHCLQKVLEGSVNPEEYLRVIRI"
BASE COUNT         4272 a         4219 c         4098 g         4914 t
ORIGIN      
        1 caggagacag acaaactggc tcgagctctt gatgacacgc gccgtgttat tttttcctgt
       61 ggcggcggaa tgcctccggg cgttcccacc gaaaacatcc tcgcttttct gaaagcagtc
      121 caatctgtct gaaaatagag agacaggaaa gtattcggtc ggaaaaagcg ttttcctcct
      181 gacagtaaca aatgctctct tctcttttta ttgtcctgaa acttttgtta tactcaaaaa
      241 ctgtaggtgc aaaccccgcc tgcacgaacc acaatacctc tgatttaaag gaattttatg
      301 tacccgacac tactcagctg gggcgaattg cgcttccatt cctatacagt gatgatgacg
      361 cttgcttttc ttattggtgc tctgggaccc aactggctaa atatgcgcag gaaagagccc
      421 tacgctgcta ctccggctgg cggtatatgg atctttattg gtggcatagc cggcgctaaa
      481 atttattggg ttatccagta cggcggctgg gatgatctaa gatatctgca attttttctg
      541 acgggcggtc tcgtgttttt cggcggtctg atcggcggga taattgcagg cattgcttac
      601 cttaaatatg cgaaaacacc cgtgattcct gctgctgata tggttgcccc tttccttgcc
      661 ttcgcccatg gcatcgggag aatcggctgt tttctcaatg gctgctgctg gggccgtctg
      721 tgtagtgcag gacttctctg gggggtatct tttccccgtg ccagctacgg gcctttcaga
      781 gagcaggtca gaaaggggct tattgacggc gcagctgact tttctcttcc tatccatcct
      841 gtccagctgt acgaaacatt gggtctcttt gttttgtttg ttgtactgat gatcatttac
      901 aaaaaacata aacatactgg agtcgtttct ctgtcatatg taactctata cggacttctc
      961 cgtctcacta ctgaaggatt cagaggagaa agcgtacgtt ctgtttttgg atggctaaca
     1021 gtctcacaga caataggtct tgccatgttt gttacgggcg ggctgctttt tctccttttt
     1081 aaatgcacta tatggaaaga ccgtaagagc gacgatgctt ctgaggttac tgttgtcctg
     1141 aaggagtctg atttatagtc atcctcctgc ggacagccag tcttcttttt tcttttacgc
     1201 atgttaccgg tgtattttcg tgacttattg gagccggtat ggaaaacttt cagatttcgc
     1261 gcttgcttga tgaaacagct ttctggctgg aaattgccgg ggagtctcct tttgcagtta
     1321 gggcgtatgc tacggctgct acaacagtca acagaagcga ccttgaaatt gcagaattgg
     1381 ttctttccca tcagccttac agcctgcccg gcatcggtaa aacactttcg tcacagattg
     1441 aagagtgcgt tctccatggc cggataacgt ttctggacga tttgcggaag cgttttcctg
     1501 agtcgctccg gagccttttg aaagtgcctg gaataggcgc aaaactcatc cgactctttt
     1561 atgaacagtt gggcatcacc acattgccct tattaaaaga gaattgcctg aacggtgaaa
     1621 tacagaagtt gcccggattt ggagagaaaa gagtccagaa acttctcgaa gcaatcgctt
     1681 ttgcagaggc acaacaggga aaataccgta ttacggctat agaggacgaa gcccgggcac
     1741 tatcgtcttt tttatctgct ttaccctgtg tcctgcgttt taaaattacc ggcaagctga
     1801 ggcgtttttc caacgtttat gaaaagctgg tttttgttgt tctggggtcg gatatcgggc
     1861 agatcaaagc agcgttggag gaaacatttg ccggcatctc ccaaagtggt tgtgttcctg
     1921 aaaagtttag ttttcgtacg gctcccggca tgctttgtga ggtgtatgtg acagaagatc
     1981 aggcttgctg ggttcctctt ttgattgaaa caacaggcag cgcacagcat gttgccgctc
     2041 tgaaacagtt gagtgcatca aaaggtcttt tatggcagtc ttctgttctt aaggatgcgc
     2101 aagggcaccc tttcaatttc gagaaggaag aggcgtttta tgctctttta ggaatacctt
     2161 actgtgaacc cgtcatccgt gaaaatgtgt atttacctgt tgtatcagac acaccggagc
     2221 tgctgaattt gggggatcta aaagggctgg ttcattgtca ctcccagtgg agtgacggcg
     2281 cccactcact agaatctatg gcgcttgcct gccgcgatct tggctacacc tatatgctga
     2341 tcactgatca cagccaatca tctaagatag ccaatggtct ggaacccgac cgaattgcgg
     2401 cgcagcatca ggaaattgac gcgttaaaca ggcgcttgac cggcatccga gttcttaaag
     2461 gaatcgaatc ggacattcgc aaggatggtt ctctggatta tgatgaagct attctcagaa
     2521 cctttgattg ggtgattgct tcagtacata atgggcttga cctggataaa acagaagcga
     2581 caagacggct gattcgtgct atggaaaatc catttacctc tgcgataggg catcccagcg
     2641 gacgtcatct tctcacccgc cccggttacg agctggatat ggaaaaaatt acagatgcgg
     2701 ctgtaaccaa taaggtggcg ttggaaatca atgcgagtcc caaacggttt gatcttgact
     2761 ggcgccatct taaagacgct gctcataaag gggcgcgctt cattatctcg ccggatgctc
     2821 atcgcatcac cggctttcag aacgttcgct acggtgttca acttgcgcga aaaggctgtt
     2881 tgtcttccga ccacatatta aactgcctcc cgcccgagga atttatggca tggcaaaaaa
     2941 aaccataaac gctgctgaag aaaaaaaaag agtcgctgcc atttaccgtc ttctgcagga
     3001 aacctatccg gaagcagcct gcacgcttaa tttcagatct ccgttgcagc ttttaatcat
     3061 gactattctc gctgcccaat gtacagatgc acgtgtaaat attgtatgta cgcagctgtt
     3121 caaggacttt caaagcgttg atgactttgc tgatgcagca ccgggcgcgc tggaaaaggc
     3181 aattcatgcc tgcggttttt ttaatcagaa agccagagcg atccgtgaaa gctgccgtat
     3241 gcttcgggca gaatataaca gtcaggtccc ggacacaatg gatgatctgc tgcgcctgcc
     3301 cggcgtcggg agaaaaattg ccaatgcggt ccttggcgaa tgttttggaa agccggctgt
     3361 tgttgtcgat acacactgca aacgtgttgc caacagactt ggttttaccc gtcatcagga
     3421 tccgggaaaa atagagatag acctgcagca gctctggcag cccgagcact ggacgatgta
     3481 ttcccagtat atggttcatc atgggcgtgc ctattgtctt gcgcgctctc caaaatgtgc
     3541 agattgtcct ctcttctctt tgtgtccgtg gcctgaaaaa gacaagaggg cacatggcgc
     3601 attctgaccc ctctaaagcc gcggtgttgc cggttgcgga aggccgcact tcttttgcct
     3661 ggattagcat actgtacgtc ggtcttgttt tcacaatcga taccctcgcc tcccaaagag
     3721 tatcttttct ctttgactgg acaatgttta gctggtcatt gtcagattta ttggagtatc
     3781 tgcccgattt tcagaaagga agtcccttac tccgcattgc cttccatttt gatctgttta
     3841 aatttttagc gtggtttctg tttcccttcg tgctttgttg gaaaaatctg gacaggcagt
     3901 ggcttctgcc gacgcataca caaaaacgcc cccggttgtt ttatttcttt tccatcctct
     3961 ctgtctttgt tgtgctgata gcgcttttct tgattccgtc gttgagggat tattacgggg
     4021 gcaatacagg tgcgcagcag aaaagctggc tggaagccgg attgtctttt tttgtgtggg
     4081 aaatgtcctg gttcccaggg tgggagtttt tatgccggta tgtgctgctt cgtgctgctc
     4141 tgcggattcg cccatcgtgg ggctggctcg tggttccgct ttttgaaggt ctttatcatt
     4201 tacagaagcc tttcgcagaa gcaatgggca tggctgtgtt ttctttattt atgactgcct
     4261 ggactgtaca gcataaaagc atgttcattc cattttgtgt tcatttcctt atagagattg
     4321 ttttgctgat attcctcttc ttgatttgag tctgcagcag catcatttta ctgctgagtc
     4381 tgggttcgtg tggcgcccag aattcctccg ccggacctga aaatggacat gaacatttgc
     4441 cggggagtat ccagcagttt catggcctcg cctgtaagat cggcggcacc gcttacaaaa
     4501 gttacaccaa tgggcggcag accagccaat tcactcgtcg gattgaacgt tggccccgaa
     4561 agttgaaacc ccagttctat gttgcgctgt gtcatacgga agccctgaat attaaaactc
     4621 tcccgcatga tggaaatctg ttcagccaga tcaggagaca tggacaggtc tattctgtaa
     4681 ttcatatccc cttccagaat ccaaatgcca tgacctgtta tagtcattcc gtcagattca
     4741 atagaaacat tgtctgtgaa aatttgatcc cctttaattt tcaaatcaga gctgagtttt
     4801 ttaaaccgca gtgcagcagg cggcaaaacg ccaaatttct gggcaaagct ggacgtaaac
     4861 acttcatata atttatgttc aatgaaatga ccattggtaa ctgaaaaact gccttcggtt
     4921 tctaatgtat tcccgggatc atcctggtcg acagtataag aaacagtgcc tgtgcaattt
     4981 ccttccagaa tctccggcaa ctccaggtgc cggataagat aaacagcggg aatctcttcc
     5041 cagttccctt ttaatgtcat gatattgtcg cgtttttcgt gatgataggc cccattaacc
     5101 tgaccatctc ccatttttcc acggaaaaac gaaaaatatg tttcatcggg gttatcatga
     5161 agctgaccgg aaaaatcttt cccctgccac ggaggaagtg aaatactgtc agcggacaaa
     5221 ttaaaaatcc aaaaagtcgg ctccgacggc ttctcttctg aatcctgctc ttcctcctta
     5281 gcacccatgg gaagaatcca tttttctgaa aagggcggca ccttggcatc cccggcatgt
     5341 atgacaaatt ctgttgtctt tggtttgttt ttgatagtgg ttattgtaac ttccacgctg
     5401 gtatctgtgc agtccagcgg gtgaatacca ccatcgggaa aaaaagaaac atgatcgaag
     5461 tggccgccca gtttggcaat ggaatcaccc ggcaacggcc caacataatt agactcataa
     5521 aaaccatttt tgcaggctcc ccctaaagca gtgtacggaa tatcaacaca cttgccggcg
     5581 ctgttgactt ccagatgcgg cagatcaacg cgggtacgag tgttctccca tttcttatca
     5641 tcgctgtacc ggtatttgag atccgcaaga attttcgtgt cttctacaaa tgcctcccct
     5701 gtaatagtca tacttttacc gggcagaatc tctgcattaa gcgacttgat cgttgcgcct
     5761 acaccaacag gtttttttag ccaccattca aatgcgacca tgcctcgggt aacatcggca
     5821 ctggctttta cagtgagggg accgcctgcc aaataggtgc ctgaggcatg gaaagcaata
     5881 tctccggcaa gttttaaatt cttaatggaa tccttcagga ttgtattttc cagcgcagaa
     5941 agttccccgg tgattttgaa cgctgctttc tccttttcca gatcataggt tgcactgccg
     6001 tacaaatcct tttctgcaag aagagcgctg aaaggctgta aggaaattgt ctttccatga
     6061 aggctgatta ctccggagac ctgagtagct tcaaagccgg ctttggcagc cttgatcgag
     6121 ccatcagaaa ccagcgcaga aagttcaggg acagcagaaa cggagtccca ggatgcgcgg
     6181 atttgctgca gccgaaacgc aacttcaggg atctctttat tggaaggctg aagggcgagg
     6241 gtaatttccg ggacagcagc ttcgactttt atagaagggt gttcgagtgc gcctgtcacc
     6301 tgacagacta tatattcatt tcccgaaaga tgtattacag gttttcctaa gcgatttaaa
     6361 gtttttggca ggtattccgc taaaatggcg gaaagaggca ggttttctcc ggttacagat
     6421 gcctgcacct ggggtgaatc tgccgaaaaa tcaataacgc cttgtccttt gagattcaac
     6481 agatctgtag acaggtctcc gctaaggact ctgagctgtt tatctgcgga gctccattga
     6541 atgacactgc gcagcgctcc attgatagag ctgaaagctg ccggaagatc cggaaaggta
     6601 aaccgggaaa tatcaagggt tgtctcaaca agcagagact tttccgtatc gccggaaaag
     6661 gaaacatttc cttccagtgc tccatccaac ccatcaggga ccggggtaaa atgacgtaac
     6721 agcgctccgt tgatgccatc aaagtgaacc tctccacgga catgtccggg gagctgcaga
     6781 tctcctgcaa cttttatttt tcctgcacta gcaccaacgg aaaacgctgt ttccaatgca
     6841 acgagcagtg cacctttccc cgaatcattg gtcagggaaa aagaaatgct gtcaaatgag
     6901 agtatgcttt tatcgtcttg ccttatctcc agagaacaat ccgaccctga taatctctga
     6961 aagggaagcc tttccaacaa gtctggcggc aggagaaaag gcttcttttg gggctttggc
     7021 tgttgcgaag aactcccttt aaagtagacg agtttcacgc cttccagcac tgcatcgccc
     7081 acagaaattt ttccccggat catcgccgga attgaaagtg ccaatttgac actttgcgcg
     7141 cgcatatgca atttatcatt atcgcccaga ggcaaaagca gtgatagttc ggaaagcgtc
     7201 agacttcgga ggccctctgt ttctacggaa gtgacgttaa aatctattcc tgtatgtgct
     7261 ttgattgttt ggagagagtg ttccctggca ttctgcaggt ggtattttag gaatacagca
     7321 ccagccgccg ttataaagga gaggataaac gtcgcaacta ttaaaaaaga gatggcatta
     7381 tgacgctgct taaagtgcac ctcaggcagt tgctactccg cgggttgttc gtctgtttct
     7441 tttgcgatcc atggcaagcc tttgttggca agaatttgcg acacaagttt ttccgtaagt
     7501 gccggattgt ccctgagata ctgtcttgta ttttcccgcc cctgtccaag attttctccg
     7561 tcatgagaaa accaggcgcc gcttttcaat attaatttct cttccagagc aagatccaga
     7621 atatctcctt cacggctgat accctcatca aataaaatat caaattcagc ctgacggaaa
     7681 ggagcactta ctttgttttt aacgaccttg acccgcactc ggtttccaac gttttcttca
     7741 cggtctttca agccggatat tctgcggatg tccaggcgca tacttgaata gaatttgagc
     7801 gcacggcccc ctgtcgtcgt ttcaggactg ccaaacatga cgccaatctt ttcgcggatc
     7861 tgattgatga aaataagaag cgttttagat ctgctgatga tggcagtcaa tttgcgcagg
     7921 gcctgagaca taagccgcgc ctgcaacccc acatgagaat cacccatgtc tccttccact
     7981 tcagccttag ggactagggc agctacagag tccacaacaa tgatgtcaac ggcattgctg
     8041 cgcacaagac tctcgcagat ttccagcgcc tgttcagcgg tgtccggctg cgaaaccagc
     8101 agattattaa tgtccacgcc aattttttcg gcaaaagaag gatccagggc atgttccgca
     8161 tcaatgtagc atgcaatgcc gcccgcacgc tgggcgcttg ccacagaatg cagtgcaagg
     8221 gttgttttcc ctgaagattc cggaccgaaa atttctatca cccggcctct aggaaaaccg
     8281 cccacgcctg ttgctatatc aagagaaaga ctgcccgtcg aaataacctg tactccttcc
     8341 cggaaagaat catctccaag acgaaccagc gtgcctcttc cgaactgttt ttcaagctgg
     8401 gatacagcaa tatcgagagc ccgggctttt cctttgtcat cattagtcgc agctgccatc
     8461 attatctcct tttatggttt actgaaaaca ttttcatatg cctaagtgtg ccataaacca
     8521 aggcaaatat caaaatattc tccggcacct ctttgattcc ctccataagc acagggtaca
     8581 atataatacg atgaaatcat acggtataat caatttttca gtgcttggcg gcggcggcga
     8641 agtaggcgct aactgctttc agcttgcctg taacgggaca cggatacttc tggattgtgg
     8701 aacccatccc aagaaagaag gactggcgtg cctgcctgac ttcagactcc ttgaaaaacc
     8761 gcccgattta tgcctccttt cccatgcgca catggatcac tgcggcgcat tgccctattt
     8821 gcagaaatgt tatcccggtg tttgctctta cagcactatt cctacccgtg ccatcgcaga
     8881 ccgtatgatg cgcagcagtg taaccgttat ggaaatgctt gccagagagc gccaaatcta
     8941 tgattatccg ctttatgatc atagtgatgt ttcgctcgca gttcggctgc ttaacggcgt
     9001 tccttacaat atcccggtag aaatcggcaa cggcacgcct atagaactgc gttttatcaa
     9061 tgccgggcat gttctgggcg gagccagcat tcttctgaaa ttccccggcc ataccgtttt
     9121 ttatacgggt gacatttcgc atcgccgcca atttttaatt ggcagccgtg agcgactgga
     9181 tgaaacagaa agagtagata ctttgattgt tgaatccacc ctgggagcca ccgaagagcc
     9241 tgaatcgtcg tcttatcaga gcgaagcaga caggcttgcc gaagccatcc gggacgtatt
     9301 gcgccagggt ggctccgcac tgctgccttg ttttgccttg gggcgcaccc aggaaatgat
     9361 caacgtcatc tgtcatttac agaggaacaa caaactgcct cgcgttccga tttatagcat
     9421 tggtctgggc agagcaattt atcagatcta cgaccgctat tctgactacc tgcatcccga
     9481 atcatcgctc acgccccttg atcatacggt tcaattaaaa aatgtctggg aaaaaggact
     9541 ggtacagaaa ctgcttctta agccctccat catcatagca acatcaggaa tgatgactga
     9601 aaatacacct tccgccttgc ttgcacaaag tatggtgaca cagtcacacc atggtatttt
     9661 ttttgttggt tatctggatc ctgagacttt gggctatcgc ctgcttcatt gtaaaccagg
     9721 cgaagcactg cagttctcct tctctgcccc tccggttgaa gtgatcctgg aaaacatccg
     9781 gcaattcaga ttcagcgccc acgcctcgcg cagatattta ctgcagatga tagagcgatt
     9841 gaaccctaaa aatgttgtct atgtccacgg cgatccggat gcgctcgtct ggatggaaag
     9901 caattccaca aataaggcac taagtttcat tccagaaaca ggccacgaat ttgttctgga
     9961 agcgtaggta tggccaaaaa aagctctccc ttttcaattt tcttctatgg agtggttctg
    10021 ttttttttcc aattaacccg cctgctccct ctttctcttt cccgaaaatt tggaattttt
    10081 cttggtgacc ttgccttttt cataataccc cgtttgaaaa aaataggcat ggccaatctt
    10141 gccatcgcat atggcgacga aatgccgctg aaagaaaaaa agcggatact ccggggcgct
    10201 gcccgcaatc ttggtatcat agcagcggag ttttcacgca ttccttctat cgctaagggc
    10261 ggccttaaaa atatgatttc tgttgaaggc attgaagaag ccctctctca taaacctgca
    10321 gtattgttgg cagcgcatca cagtaattgg gaatggatgc tccccgtcgc ttacgatctc
    10381 ggcttgagga cagccgttat cgtgaatcca tttcgcaacg cttttctgga tcattttgtg
    10441 aatcagtatc gtttgtctgg aggaacaatc accgtaagaa aacaaggtgc cgttcaggag
    10501 atggaaaatc tttttaaaaa tggcgttagc ccttctatgc tgggcgacca aagcccccga
    10561 aatggcggtg tcccgattaa atttttcgga tctcccgcct gggcgtctat cggtccggca
    10621 atgatcgcca tgaaaacaga cagccccgtg attccggcat caatggcaag agacaataaa
    10681 gggcattata cattgaaatt ctttccggct attcacatgc gaaatagcgg ccagtatctg
    10741 gaagatcttg tttttaatac acagcgctgt caggatgcgc tggaaactat tattcgcgct
    10801 catcccgagc aatggctttg gttccatcaa cggtggagaa aaagagaacg tcttgagaag
    10861 gaatgggcgt ttcgtcttga gaaaccatcg caagactctg ataccgtcca aagtctgaaa
    10921 gatcaggaat cagcataatg tctttattca cagcctatgc ttccaaatac tccggcctcc
    10981 cctatcttgc ggttgcggtc acagatcgcg tttcacccga cagcgtggca ccgtataaag
    11041 agagccgctt tcctctttgt atagaattgc gcgtggattt gtgtgagaaa caggatcctg
    11101 aatctgtgaa aaacacactc cgttcttttg ccgcctttcc caggctgctt actattcgct
    11161 cctcttctga aggtggaaac tggcaggaga gcgatgcggt gcgccattcc ttatattcag
    11221 aaacactttc cgatgccgaa ggtattgata ttgaactgag tgccaggagt atttgcgcgc
    11281 ctttaatcag ggaatgcaaa gccttgaata aaacagtgat cggttctttt cacgacttca
    11341 gtgccacgcc gtctattgaa aaattggacg cactagttct ggaaggcaaa tctcttggtg
    11401 tggacgctgt aaaagttgct gctcactgtc attcttcaga cgatttgaga cgtttaacgt
    11461 gttttctcct gagccatcct caggacgcac ttattgtaat gggaatgggc agggatggga
    11521 cgccaagccg cctcttcttt ccttttttgg gttctttgct tacctatact tttgtaggta
    11581 ctccaagcgc gcccgggcaa aagaacctgg acgaaatgct tcactttttg aactatttac
    11641 tgcccgagtt ctgactgttt tttagcgctg atattgtatc aacatgctta atatgataca
    11701 ctttcctgaa agaacaactt cgagggttac gcatgaaaaa tatcctttta cctttggtcc
    11761 tgctatgtat attggtcggt tttgtggtgg cgtgtgctcc ggaaccgccg ccgccgccgc
    11821 cgccgccgga agaccttccg ccacccgagc cctctcctga agaatatcgt cagcaggttc
    11881 gcagcatatt aagcagctat ttgatgaccg gaacctctgc gcctgatgat gctgttcttc
    11941 ctgaaattct caatcagacg caagcgcgcc gtgcgcagct tattgccact gaaaacggca
    12001 ggcaggcact tcatcttgtg acagctgatg tggacgaaag ccttaaactg gcacgggaag
    12061 aagaaaggtg gcggaaaatt tccggattgt gccggattta caaagcgctg cagccggata
    12121 gcaaccggta cgaaaaactt cagagttact cagatgaaat ggtaaagcgc cccattctga
    12181 ctgtaacagg ctttattgaa cttgacgatg aactctatgt ctttatcgat ctttttgatc
    12241 cgacgacagg ccaaacaaaa gcttaccgtt gtcgtgaagg cgaagagttt cacgaaagag
    12301 acaacaaaac ggtcatgcgc ctcgttcgaa tcattggaaa caagcaatcc attgaagtgg
    12361 aatatctgcc ggcaaattat tcctggcttt gcatcggacc caaagaaaga agccgcctcg
    12421 gccccgatcg aaaagcagat tgacatgtct ttactcgagg tactttttat atggtttaaa
    12481 aggatagtat tttgcctaaa tccttaaaat tacctccttc ggtcatttat attgtcattc
    12541 ttgtagcact tgccggcact ttggtgtata tcaacacttt tcacgctgat tgggtctggg
    12601 atgatgtgtc ctcagttttg ctgcacaaac atgtgcaaac cccgtccagt gtgtttcaat
    12661 tgtttctgga agaccaacat gctttcgggc gtggacaggg gaatttctac agacctcttg
    12721 tatcgttaag tttcctgata gactaccaat tggcaggtgg cagtacacca atagagggac
    12781 agtcatcctt tgtccctcag atctcaccct ttgtttttca tcttacgaat ctggcatggc
    12841 atctggcagc ggctttatgc ctgctgggtc tcctggctgc attaaatgcg ccctattttg
    12901 ttctgcttgc ggttccgctt atttatgtgc ttcatccttt gcataccgaa gcggtcgcat
    12961 acataagcgg aagagcagat atgatgtctg ccacaggcat ttttggagcg ctgtggtctt
    13021 ctgtttgtta tttgaaaagt gcgggcagaa gacgttatct gttttttatg attctttgtt
    13081 ttacaggcgc cttattaagc aaagaatcct ctttaatctt tccgtttctg cttaccttat
    13141 tactattgac gacccatcgg gaaccgttaa gtgaatctct ctcagattca tcgaaaaaac
    13201 attgtaaact acatctcttc agtgcgtcct ttattattct gctggtatat atcctgcttc
    13261 gctcaacagt actgcggttt gccacggaaa gttctacagc cagtgcagcg ttttccgaaa
    13321 gagcttttga aacttttcaa tcgcttggca tttatttaaa actgcttttt gccccctttc
    13381 atttgcacat ggagcgaacg ctcacgcatg cagggagcct tcattttctt gcaggttgtc
    13441 ttttctttgt tgctcttctt ggtgccattg cctgttcttt cgccaaaaaa caatcgttta
    13501 tcggcattgg tgccttgtgg tttctgataa gctggatgcc tatttccggt ctgtttcctt
    13561 taaatgcacc gatggcggag cactggatgt atgtgcctat ggcaggtttc tggctgtgcc
    13621 tcgccgcaat actgaaaccc attgtgagtc atcctcaagc gaaatttctg ctgtatggca
    13681 ttcttggctt gttttgcctt ttcctgggca ctttaaccat tcagcgtaat ctggactggt
    13741 ctgacaatga acgccttttt ctggctacgc ttcgcgagaa tccggagagc gcccgtgtcc
    13801 attacaatct tgctgtcacg tatagcgacc ttgttggtaa tgacgcagct gcaaagcgcc
    13861 attatgaaat tttcctgaac ttaagaagca aagaaagagc ccagcaccct gataaagccg
    13921 gtttgattat tgatgatgaa atagatgcca gattggcgct tggaaagtca ttaatgaatc
    13981 tttccgccta tactgaagcg ctgggtattt acactccgct gatcgctctt gctgaatttg
    14041 aggtatggcg tcccacagct gctcttgcgg cactgcgctc gggcttatgc atgcttgcac
    14101 tgggcgaaat tgctcaatcc aatgcctact tccaacaggc gatgacgctg gaacctcttt
    14161 atgctccgga tgtggagcgt atcatacgag gtgaaaatag ttttcatatt tattaacctg
    14221 tgaggtgtct tatggctgag cggcttcatt tctccggtat cggcggcact gccatggttg
    14281 caggagcacg tattgcgctg gaggctggat gtgaggtgcg gggaagcgac ggcgttttat
    14341 atccccctgc ctctcatatg gttcaagcgc tgcagatccc tgtgtactcc ggatattcag
    14401 ccgataacct ttcctggtct ccggacactg taatcatagg caatgcgctc agcagaggca
    14461 acccggaggt tgaatacgtg ctgagccacc ggctgacata cacaagcctt ccggagtggc
    14521 tcaaaagaaa tgttttgcac gcacggcact ctatcgtaat ttcaggaacc cacgggaaaa
    14581 caactacgac cgctttaact gcgcacgtac tgaaagcgca tgatccaggc acaggatatc
    14641 tgatcggcgg tgaagcactt ggcttttccc attcctccca tttaggcaag gaaggcgccc
    14701 ccttcgttat cgagggagac gagtacgaca ctgctttctt tgataaaaga gccaagtttc
    14761 tgcattattt gcctaataca gccgttgtca cttcgctgga gtttgatcat ggcgatatct
    14821 atcatgatct tggagacatt gaaatcgcct tccagagaat gttccggcag atccctgctg
    14881 agggtctggt tttgttgtgt gccgataatc acgccctttc tttagccacc cattcttttt
    14941 cgtgcgtgga aacatacggt tttcacgaaa atgctctatg gcgtggaaac tacactgctg
    15001 aagcctcaaa ttgccaggag atgcaggtat tttataaaca aacgctttgg ggctctgtcc
    15061 cgcttccgct ttcagggcag cacaaccacc agaatattct cgcagcgata gctgtagctg
    15121 cctcgcttgg catgaaacag caggatatcg ctgcggcaat tgcctccttc aaaggggtca
    15181 ggcgaagact tgaaactttt cttgaatcgc agggtatatt gtttgtcgat gattttgccc
    15241 accatcctac ggctatcaaa gggagtatag ctgcagcaaa aaccaaatgg ccgaagcggc
    15301 gtgtgattgc cgttgttgaa ccccgttcca acacaatggt aaccaaccgg tttcaggacg
    15361 acttccggga tgcgctcaag gaggctgatg ttgtgttttt gggtccaata cacagaatca
    15421 gctccctgcc cgagtctgac cggttaaatc gcgaggaaat tgttcagtct ctgactgcct
    15481 ggggtcggga ggccgttttt tttgatgagg tgcctgaact tgtgcacgcc ctccacaaca
    15541 ctgcacgcca aaacgatgtt gttcttatta tgagcaacgg tgcattcggc ggtatttaca
    15601 cccttctgaa agaaatgtat ttatagggaa aataagactg aagggttaaa cacgcaccgc
    15661 ttaatttgtc atgaaaagtt ctggagagag aggctttccc tccggcggga taaggctgtc
    15721 tttaattaag tccggaagtc cttccatggg cggggctgtc ggaaaattat ctgtttcagg
    15781 caccaggcct tcttttaaca atgccaattt agcctcgtcc atgccgacat acttttcctg
    15841 catttgcgaa aatacagcct cagcgctttc tttcacagta tcgtcccgca cgctttcttc
    15901 tgaaacatgt ccggatatac cggctgccag ggcctcgaca tgttttgtca ggcgccgcag
    15961 cgcatctctt gattctctgg aaagcggtat ttcgccgttt ttaacagaaa gtatgtctac
    16021 cgacgcaccg tttttcctgg cttcggtgac ttccgttagc gcgtcaaata tttttagata
    16081 ctgcgtgtcc agaaaggtgt aatagagttt aatggcggaa gcctgaatat caggcatctc
    16141 gttgggatgc gaggcgagac gcagcatcgt ttcatggaac atttcatccg catctttgat
    16201 aacaccggcg gcaacagcgc taacctctcc ggtaacccct ttcgttttcc gtctttccag
    16261 cgaaaaaaga agctcaggca gcgtcaggga taacagcgat tcgggcttgg cggaaccaaa
    16321 cacctcttcc ataagaaatt ttctgaaaac aggatcttct ttctgatttt ggataattgt
    16381 aagtatttcc cagatatctc tttggtcttt aattaaaaga ggcagatact ctaacgcaat
    16441 cgtgacgttg tttaaaactt gctgttcaat ttcaagagga tctttgccga gagaactttc
    16501 cattcgggca acttcgacgc cctcacgggc agagcgaagc agatcgattg tgcgtaaatg
    16561 acggaatgac tcaaaagtat attcaagccc gggattctcc agtcctttat tgatgcccag
    16621 aggtctggga ggccttttgt ctatctgttc agccacttca tcaaccattt gccaataggg
    16681 atgtaaactc cgggcagtcg gctccatttg tgcgaaggca caaaccgcaa aaaaaataca
    16741 taatgaggaa attggcactg taatcaacaa tttttgattc agcaacggca tgatcgtttt
    16801 tcacttttgt gttaagtaaa agattccaca ggaaaaaagc actgtatttt aaaactatat
    16861 tctaataacg cgcaaatatt cttccgggtt cacactgcct tccagtactt tctgaaggca
    16921 gtggcttgca agagtctggt agccagccat tttagagatt ttatctgcag acgcctcgtt
    16981 aataatttct tccctgatct ccggggtaat ttcgaggact tcaaaaacac ctgttctccc
    17041 ggagtgtcct gtgaagtggc aaatagggca cccagtgctt cggtacaatt tctttgtgtt
    17101 tgccggcagt cccaaggaag ttaacagacc aatctccggt ttaaagggtt ttctgcaatg
    17161 agaacacatt ttgcgtacca gacgctgccc gataacagct gtcaatgccg ccgcaataag
    17221 aaaaggtggc acgcccatgt ttctcaatgt gctgattgct tctatggcat cgttggtatg
    17281 taaagtgctg aataccagat gccctgtcat tgcggcgcgg attgcaatct gtgctgtctc
    17341 agggtcacga atttcaccga caagcagtac atctatatcc tggcgcagtg ctgcacgtaa
    17401 ggtgctggca aaagtcagat tgatgtcggg atcgatctgt acctgattaa ttccgggcaa
    17461 ctgatattcc acaggatctt ccagggtaac aatgctctct gtc
//
LOCUS       DAOPWB010000066        17314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3347_length_17314_cov_5.789617, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000066 DAOPWB010000000
VERSION     DAOPWB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17314
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3347_length_17314_cov_5.789617"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKN92_07555"
     CDS             complement(<1..427)
                     /locus_tag="PKN92_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNZ48685.1"
                     /translation="MTHSGSIVLAAINARYAHVAFGLRRLQAHLGCYQENTQLLEFTL
                     QQSAHEIAEAILARKPRLVGLSLYIWNIEIATAVAAVIRAVSPETVLVCGGPEMTGDP
                     ARSPVAPFADYIIRGEGEEAFLTLVHTLLQGHVPPDKIID"
     gene            complement(431..1033)
                     /locus_tag="PKN92_07560"
     CDS             complement(431..1033)
                     /locus_tag="PKN92_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48686.1"
                     /translation="MKAVRFSGIFMFALSLCILASFVGCGKKEAPAPAAPARETAAPA
                     APAKAAPAPAAQPDTKPEPEAAANAVEAPETPAPAVTTSENEAPQTVKTALASLPADF
                     PKDVPIFENSTIEAAMSIGGEQHQIVGNAHAGLDEVVSFYKKECAARGWKETMTMAQG
                     GEEAASFSTYEKDGRAMTVTVAQDEEGVHFSLSVASSEAE"
     gene            complement(1085..1576)
                     /locus_tag="PKN92_07565"
     CDS             complement(1085..1576)
                     /locus_tag="PKN92_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48687.1"
                     /translation="MLKQALPLILITAVVTATAVLFLTQKKEAGQPGENTSAAPVVDT
                     SADNTPPATEADAEHVNQDIRISKDMVDALLLGVTVEEVEALWGRISDEMESEYDPGI
                     KGYTSPYAILWHTWNNPDRTRVKLGFINRKLDRKHFYYRDGNFTTSEVDLKAIAEEKP
                     GRR"
     gene            1783..2487
                     /locus_tag="PKN92_07570"
     CDS             1783..2487
                     /locus_tag="PKN92_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNZ48688.1"
                     /translation="MKARIVKTVYVETAHRLFPASEDTEARYSGHSYRIDLMAEGTVH
                     PAYGWVVDYTDLKALFEPLRKQLDHCCLDHIPELRGHHEPKDIEAWINESLRPWPDWF
                     AGVRVSFAVPPGFHPVMLPPDPVQDLPARLAFTFSAAQSLPQLPEEHPCHNLHGHSYQ
                     VEIAGKAEALLEKTARLLFDYFDGRYLNTLPGLEQATSERIAACVWQMVEEAGAAPFF
                     VAVQETPSNRCHYFGG"
     gene            2503..2865
                     /locus_tag="PKN92_07575"
     CDS             2503..2865
                     /locus_tag="PKN92_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNZ48689.1"
                     /translation="MMRFSVTPEKQQQLEERMAACGLREEDFEERFSASSGPGGQKTN
                     KTATCTTLLHRPTGLTVKMQKARSQSLNRYYARKRLCELMEEMQLGALSPAALEREKI
                     RKQKARRKRRIGKKSQNE"
     gene            complement(2910..3509)
                     /locus_tag="PKN92_07580"
     CDS             complement(2910..3509)
                     /locus_tag="PKN92_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNZ48690.1"
                     /translation="MAVRVLYLIRHGQHDLNNPSGSSLGGPLTPLGIRQAEITAKTLA
                     MKPISSIHSSSLNRADQTAKIIAKEFPAMNVQTTDLLWECIPTLTPKLQLEMPNYNDA
                     LAAQDRRRAEIAFRRYFKVAKRSDRHDIIICHGNLIRFFITLVLKADPSSWVRMDLAN
                     CGISQVLIQPEGDMALLCHNDWSHLPKELRTSTLRPPEK"
     gene            complement(3642..5357)
                     /locus_tag="PKN92_07585"
     CDS             complement(3642..5357)
                     /locus_tag="PKN92_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48691.1"
                     /translation="MLAYQFFAIIRVKFLLIRGSLERISPLGKVLLLLLFLGCGLLST
                     ALSAALFYAARRTSADTGTGALLFLLDGTASLFLFFFLWSVLIELQRNDGIALDKFLA
                     LPIRPVWIFLFNFLMFICTPLFFFSLPPLVALAAGLIPRCGIFSLFPIVLFALLFITA
                     LNAWAYWLCSVLALWMINKRRRRLIFVIVPVVFVILAQLPTLLLRFFLTHGQQRFDVA
                     AMESYVPGLLSIHQTIPFLLPIYGLWNQLTHTNPNAVVLSFLASLLFIAAGLYLGYAT
                     TMRYYRSGHSESPAGNGSDRKSKKDKGLSRTVHTFFPAVAASDISCLTLTFFRSYARH
                     PHMRMLFLMPASMGVFMIFLFKGGGLSGTGSGMRWVPHFWLFLPFLNFSFFLMNIFGA
                     EGRAFQTLMLLPVPRYRYLIAKNLAMAPFLFGLSFFLFAATLFFYPMSIAEITVVLAL
                     VFHLFLVFSATGNLYSLYFPQPVQRDAMRAPVSRVRLLGHMIVLTFATAVMVLPASLC
                     FAFRHSEILTWPVGNCSIMPGLSGSLILFILSCIYYRAVLYRAGDLLQEREQLIFTIL
                     SQENE"
     gene            complement(5358..6116)
                     /locus_tag="PKN92_07590"
     CDS             complement(5358..6116)
                     /locus_tag="PKN92_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48692.1"
                     /translation="MNICIESLGLTKRYGGRNVVDQLHFRVPKGSFYGFLGPNGAGKS
                     TTIKMLTGTLAPDAGSARILGFDLQRSSLEIKRRIGVVPENLCLFENLSGPEYLRFIG
                     RMYGLPGTVVDKRCDELLELLSLHTAARTLILEYSHGMRKKLALCAAVIHDPELLFLD
                     EPFEGVDAVASRIMRDLLSRLRKRGVTVFLTSHILEVMEKLCTHIGILCEGHLAKQGT
                     IEDFQEEGGLDHIFLQIAGAHTAASKNLSWLGNE"
     gene            complement(6118..7053)
                     /locus_tag="PKN92_07595"
     CDS             complement(6118..7053)
                     /locus_tag="PKN92_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48693.1"
                     /translation="MPLPLFVSAIMLLFPALLLPLQAEAAPDSPEIPSLEGTQEEVES
                     RVRTFDKLMMIQVRRLEDTAGKEGQPDNRPGTEEARSHAAACLSAIQAAYEEALLRFP
                     DSAVLHNFYAEFLYDFQGKTDDAQKHWRQAVALNPRLGRALNNLGMLYCHVGQYGQGL
                     EYLWQSLKEEPDNPDFLFNTVQVYLIHPESVMSTRKITQRKIYEEAMSMSERAVKNAP
                     RAFDILQDYALNFYLAEKFDAKPDWKKAAKAWQEARKQAKTKDELFNTWLNEGRVHIR
                     QHKTSAARTCLEEARALRPESAVLKALFENLEDAH"
     gene            7241..7900
                     /locus_tag="PKN92_07600"
     CDS             7241..7900
                     /locus_tag="PKN92_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNZ48694.1"
                     /translation="MAEWTALEQEVMDDMLRRRPDLKCCEEAMRQFNGALKNCYDRGG
                     TFFTCGNGGSCTDAQHIVGELCKSFERKRPLTNEIREALSSQPLGNELADNLERGLPA
                     VTLGLNSALKTAVENDIPLRDIAFAQELNALIRPGDMLLAISTSGNAKNCLMAMSVAK
                     AYGGIAAALTGPRDSNMARHADVIIQVPGGSTKEIQEGHAVVWHTLCCLIEVAYFPEM
                     R"
     gene            7948..8898
                     /gene="mdh"
                     /locus_tag="PKN92_07605"
     CDS             7948..8898
                     /gene="mdh"
                     /locus_tag="PKN92_07605"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNZ48695.1"
                     /translation="MSQKKAIKIACIGAGNVGATAAQFCLELELGNVVLTDIVEGLPQ
                     GKALDMTQAGPIRGYGSVCTGTNDYADIAGADLVIVTAGLPRKPGMTRLDLLEKNGSI
                     IAGICDAIKTHAPDSTVIIVTNPLDVMAYLAYQKLGFPKNRVLGMAGCLDGARMSAFI
                     AMELNVSMKNINTMVLGSHGDTMVPLPAYTTVSGVPVTELIAPERLEAIIARTRDGGG
                     EIVRLLKTGSAFYAPAAGAVRMAQAILRNERQLLPCAALLEGEYGLSDVFVGVSCILG
                     QNGIESIQPLALRDEEQAALERSAAEVREGIDGLRSLGLL"
     gene            complement(8971..10431)
                     /gene="nhaC"
                     /locus_tag="PKN92_07610"
     CDS             complement(8971..10431)
                     /gene="nhaC"
                     /locus_tag="PKN92_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HNZ48696.1"
                     /translation="MIRTEPAKVRFSVALLPCAVLIVLVMLNAWCFGEDASSGPNQMA
                     LLLSGVLVVTLGWILLALSYKDIEAKAIQSIVLAMEAVLILLTVGCLIGLWILSGIVP
                     SMIYYGIQLFTPAVFLPSACLICSIVSLAVGSSWSTMGTVGIALLGIGRALSFPDPLV
                     AGAIVSGAYFGDKLSPLSDTTNLAPAVSGSELFTHIRHMLYSTIPSFLIALLIFTGIS
                     LFYTPGAYNNAAVSEILDGIAASFSVRWYLMLIPLLVIVMAAKGIKAIPALTIGTLLG
                     AAAALLFQPQRFLSPAGESLSALEQYKMILLTAYEGFRLESGQPLTDTLLSRGGMAAM
                     YDTVSLILAAMFFGGAMEATGLMERLAQAVLSKVRGIATLIASTIGSCILCNIFLSDQ
                     YLAIVFPGRMFRSAYQHYRLDVRNLSRALEDGGTLTSVLVPWNTCGAFAAGVLGVATF
                     AYLPFCFFNLINPVVAVVLAAFHISIKYEEEKPAED"
     gene            complement(10539..10727)
                     /locus_tag="PKN92_07615"
     CDS             complement(10539..10727)
                     /locus_tag="PKN92_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48697.1"
                     /translation="MPVHPVDPEDERCYGSCFACIPTCPRHPENQELSREQEAENLRS
                     ASSTEAPASREETGQEDA"
     gene            complement(10708..11949)
                     /locus_tag="PKN92_07620"
     CDS             complement(10708..11949)
                     /locus_tag="PKN92_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNZ48698.1"
                     /translation="MKTYIAPLSAALFLFTLTASSGLYAEGAPLYRMEDNVRSHWVSF
                     ENPDAAKGSGGTANGGAKGAAFQVIKAGEEKVLMDVSGSGLIHRMWCTLPKRSPRDLR
                     SFVLRMYWDQAEKPAVEVPFGDFFGAILGEVRPFHNDLFSSPEGRSFNFTIPMPFQTH
                     ARITFTNESDEDLPQFFYDINYSEMKFEEKPLYFHAVWHRERYTELRRDFEILPRTTG
                     KGRYLGTHLGIIGHPDNLGWWGEGEVKIYLDGDTALPTLIGTGTEDYVGTGYFQGEFA
                     HPYQGSLLIDNVNQYWTMYRYHIPDPVFFHQDIRVTIQQIGGAMQEYVKEMLSKGVEI
                     LPISVGAQDGFVPLLESEPPRSLNDPGIPYGWTNFYRRDDVCAVAFFYLDAPENQAPP
                     ISPLAERLTAIGERRDAGTPR"
     gene            complement(11995..12594)
                     /locus_tag="PKN92_07625"
     CDS             complement(11995..12594)
                     /locus_tag="PKN92_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ48699.1"
                     /translation="MFLAVIGDILSNACSLQTVLDHLEEEGIREVVHTGNICLAPEGA
                     SACVKLLRERAVTAVQGRLDRALVSRKAKAKRLPEAAELEKSYALLDSSAIEFLNGLP
                     RKRSFIREGVEWLLCHGAVNSPSETLTKDTSFLKFQRQREACTARIIISGGSTEPFSV
                     DADGTLFASAGTLLSREGQIRYTLIDTEVSPFEARVVTL"
     gene            12987..13301
                     /locus_tag="PKN92_07630"
     CDS             12987..13301
                     /locus_tag="PKN92_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ48700.1"
                     /translation="MAIKKAKATDKALTKAATLTVLAEETGLTKKDVANLLDVLANLA
                     YAQAPAGFTLPGLGKLKLVQRAARTGRNPATGEAIKIPAKKVLKFTIAKAAKDAVCPV
                     KK"
     gene            13441..15753
                     /locus_tag="PKN92_07635"
     CDS             13441..15753
                     /locus_tag="PKN92_07635"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNZ48701.1"
                     /translation="MYRKYTGLAIIAAVLFAGCTHLPGLTSLPAAPEPLLIAENGVTP
                     MKIVVSESAPEPTRHAAQELRHFLKEITGADLQVVDDTTPAGESEIWLGNSRRYSDNN
                     ILVDYKVFGNEGYLIRTRGRNLLITGGEPRGVLYGVYGLLEDHLGCRWFTPEISSIPK
                     ANVLAVLPIDEVRVPALEYREPFVYDCWDGDWAARNRMNSQSARLGSQHGGKVQYQGF
                     VHTFDLLVPPKEYFDTHPEYFSLVGGKRLKERTQLCCTNEEVIHLVTEKVRRLMAEHP
                     EATVFSVSQNDWYNYCECEHCTALAEAEGSQIAPVLHLVNHVARAVKDEFPDKLIDTL
                     AYQYTRKAPKTMRPEPNVIIRLCSIECDFSHPFEDRLTPENRAFCDDLENWSRIANRL
                     WIWNYNTSFHEYLVPFPNLRVRGPNIRYLIANNVRGIFEQDVYNTPHGEFSALSGYLG
                     AKLLWNPEYDTDLAINEFLNGVYGAAATPIRLYLDLIHDAVKEPSTSMNIWISTDEPF
                     ITDALLERADALFDIAEKAVPDDPVALEQVRIARLSVDYTILSRTQRMPGKAYVLDHA
                     RGTVKASPEFKARAARFFDVAERCGFTQLWEVGGLLSEYKKEIYALFEEHAAGTLAAP
                     SEVPPLKPGAFFRYYKMYCEALPDFSALSPTEEGVVSALDLEPGKKYQPTADAYLYTG
                     YIEIPKNGVYAFSVNSNDGSRLKINGEVIVDNDGLHKATSVMGFRLMEKGFYPFELAY
                     FDAGGGSALEVQYAGPGIPFQPLAGAILYH"
     gene            15805..16491
                     /locus_tag="PKN92_07640"
     CDS             15805..16491
                     /locus_tag="PKN92_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48702.1"
                     /translation="MNDRFGWLGFTTGLLFVCFFALLFHLGFQKASLDKERKPSVSGG
                     NTVAKTAGATLQAIDGPPTQEKIDALLRGDSPDAANRETATPERKKEQLPPAEAVTVR
                     RSLPARNVVQGGIVDTLLHVTKRDPLPPEAIIIRESLPDGWSLKEVAPGVLPPDLVPE
                     PGTEKDLEFRWLHPARFPIVLRYSVQVPADAAEKAEWNGAVTWIYDNVPNTSVLPVTE
                     LHISAEKGGQ"
     gene            16488..>17314
                     /locus_tag="PKN92_07645"
     CDS             16488..>17314
                     /locus_tag="PKN92_07645"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNZ48703.1"
                     /translation="MNSDLIIPPARAHSGASVSMQGKVVLVRCEGYEEAAVYSAVQRG
                     VDLLGGISRFFQAGETLLLKPNVLSGKSPDRAITTHPSVFQAVARLFQEGGASLTYGD
                     SPGFGKVETVVQRAKLSSVAEKLGIPLADFSEGRTVSFPEGAISKQFTIAAGVLAADG
                     IISLPKLKTHALTRLTGAVKNQFGCIPGLRKGEFHARMQRIDQFAAMLVDLTRMLSPR
                     LYVMDGVVAMEGNGPGNGTPRPLNVLLFSTDPVALDAAVSRIVCLDPALVPTIVKGQ"
BASE COUNT         4158 a         4502 c         4816 g         3838 t
ORIGIN      
        1 catcgataat cttgtctggc ggaacatgtc cctgcaaaag agtgtgcacg agggtgagaa
       61 aagcctcttc accctcgcca cggatgatgt agtcggcaaa gggcgcaaca ggagagcgcg
      121 ccgggtcgcc ggtcatttcc gggccgccgc agaccaatac ggtttcaggg ctgacggcgc
      181 ggatgacagc ggcaacggca gtggcgattt cgatattcca gatgtacagg ctcagcccga
      241 ccagccgggg ctttcgcgca agaatggctt ccgcaatttc gtgagcggac tgctgcaggg
      301 tgaattcgag gagctgtgtg ttttcctggt aacaccccag atgcgcctgc agccgtcgca
      361 gcccgaaagc aacatgggca tagcgtgcat tgatagcggc aagcactatg gatccggagt
      421 gcgtcaagcc tcactctgcc tcggaggacg ctacggacaa ggaaaaatga acgccttcct
      481 catcctgggc aacagttaca gtcatggctc ttccgtcttt ttcataggtg gaaaagctgg
      541 cagcttcttc gccgccctgc gccattgtca tggtttcttt ccacccccgg gcggcacatt
      601 cttttttgta aaaactgacc acttcatcca gacctgcatg ggcattgccg acaatctgat
      661 gctgctcgcc gcctatgctc atggcagctt caatagtgct gttttcaaag atgggcacgt
      721 ccttcggaaa gtcggcagga agagaggcca atgctgtttt aacagtttgc ggggcttcgt
      781 tttctgatgt tgtaacggca ggcgcaggcg tttccggtgc ttcaacagcg ttggcggcag
      841 cctccggctc cggtttggtg tcgggctgag cagcaggggc tggcgccgct ttagcagggg
      901 cggcaggagc tgctgtttcc cttgccggag ctgccggcgc aggcgcctct ttcttgccgc
      961 atccgacaaa agacgccagg atgcaaagag agagtgcaaa cataaaaatt ccggaaaacc
     1021 gcactgcttt catgatcgtt tccttttcaa gttttataga gatagtgtta agtcgggaag
     1081 atcttcatcg cctgccgggc ttttcttcgg caatcgcttt cagatccacc tccgacgtgg
     1141 tgaaattacc gtcgcggtag taaaagtgct tgcgatccag ttttctgtta ataaatccaa
     1201 gtttaacccg tgttcgatcc gggttattcc aggtgtgcca taaaatcgca taaggcgatg
     1261 tgtatccttt gatgccggga tcgtactcgc tttccatttc atcagaaata cgaccccaca
     1321 gcgcctccac ttcttcaaca gtcaccccca gcaaaagggc atccaccatg tctttgctga
     1381 tccggatgtc ctggttgaca tgttcagcat ctgcctcagt ggcgggagga gtattatccg
     1441 cactggtatc gacaacgggc gccgcagacg tattttcacc gggctgtccg gcttcttttt
     1501 tctgcgtcag aaacagtact gccgtggcag tgaccactgc cgttatcaga atgagcggca
     1561 aggcctgttt taacatcagt cgcatccttc ctgaattgcc gccggggcga acccggtttt
     1621 tgcctttcct gatggtgcca taaaaacgta atgcggaaaa ggaacgtggt atacttgtgc
     1681 cggcagttcc tgtccgctcc cttccgtgct gtcctgcctg tacggcatcg ccacggttct
     1741 tttttcagaa tcttaacata gagacaataa agaattcccc ttatgaaagc acgcattgta
     1801 aaaaccgtgt atgttgaaac ggcacaccgc ctgtttccgg catccgaaga caccgaagcc
     1861 cgttacagcg ggcacagtta ccgtattgat ctgatggctg agggcactgt acatcctgcc
     1921 tatggctggg tggtggatta tacagatctc aaagcactct tcgagccgct gcgtaaacaa
     1981 ctggatcatt gctgcctgga tcatattccg gagttgcgcg ggcatcatga gccgaaggat
     2041 atcgaagcct ggattaacga atcactccgt ccctggcccg actggtttgc cggagtgcgt
     2101 gtttcttttg ctgttccgcc cggatttcat cccgtcatgc tgccgccgga tcctgtacag
     2161 gatctgcccg cccgcctggc ttttaccttt tcagctgccc agtcgctgcc ccagctgcct
     2221 gaagaacatc cctgccacaa tctgcacgga cacagctatc aggtggaaat cgcggggaaa
     2281 gcggaagcgc ttctggaaaa aacagcacga cttcttttcg actattttga tggtcggtat
     2341 ctcaataccc tgccgggact ggagcaggca acctcggaaa gaattgccgc ctgtgtctgg
     2401 cagatggtgg aagaggcagg ggcagcgcct ttttttgttg ccgttcagga aacaccgtcc
     2461 aaccgctgcc actatttcgg aggctgaaag agaaacggac agatgatgcg tttcagtgtg
     2521 accccggaaa aacagcagca gctcgaagaa cgcatggcag cctgcggtct gcgtgaggaa
     2581 gacttcgaag agcgtttttc agccagcagc gggcccggcg gtcagaaaac aaacaaaaca
     2641 gccacctgta ccacgttgct gcaccgaccc accgggctga cagtgaaaat gcagaaagca
     2701 cgctctcagt ccctgaaccg ctactatgcg cgcaaacgcc tttgcgaact gatggaggaa
     2761 atgcagctgg gcgcactgtc cccggctgct ctggaacggg aaaaaatcag aaagcagaag
     2821 gcgcgcagaa aaaggcgcat cgggaaaaaa tcgcagaatg aataagccgc aaagagaaaa
     2881 ggcagagggc agggaaggcg caaaggcgtt tatttttccg gcgggcgcag ggtagatgtg
     2941 cgcagttctt tcggcagatg cgaccagtca ttgtggcaca gcagcgccat atctccttcc
     3001 ggctgaatca gcacctgaga gataccgcaa ttcgccagat ccatccgtac ccagctgctg
     3061 ggatccgctt tcagcaccag cgtaataaaa aagcggatca ggttgccatg gcatatgatt
     3121 atatcgtgcc ggtcgctgcg cttggcaacc ttaaaataac ggcggaaggc tatttcagcg
     3181 cgccgccgat cctgtgccgc cagtgcatcg ttgtagttcg gcatttcaag ctgcagtttg
     3241 ggtgtcaacg taggaatgca ctcccagagc aaatccgttg tctggacatt catggcggga
     3301 aattctttgg cgataatttt cgctgtctga tctgcacggt ttaaagagct ggagtggatc
     3361 gaagagatgg gcttcattgc cagcgttttt gcggtaatct ctgcctgcct gatgcccagc
     3421 ggcgttaatg gtccgcccag agaagaaccg cttggattat tcagatcatg ctgaccgtgc
     3481 cggatgaggt agaggactcg gactgccatg aactttcatc tccttaacag atagtcacac
     3541 cgtacaagtt caaaatgtga tcccggagag tgcaccaacg catactatta atcataattc
     3601 acaaaagagt ataacagtct ttaacgttat tataccaatt gtcattcatt ttcctgtgac
     3661 agaatggtaa aaatgagctg ctctctttcc tgaagaagat ctccggcacg gtaaagcact
     3721 gctctatagt atatgcacga aaggatgaat aaaatcaaag agccggataa gccgggcatg
     3781 atactgcaat ttccgaccgg ccacgtcagt atttcgctgt gccggaaggc aaagcagagc
     3841 gaagccggga gcaccatgac tgccgtggca aaagtaagca caatcatatg cccgagaagc
     3901 cgaactctgc tcacgggagc acgcatggca tccctctgta cgggttgcgg gaaatagagc
     3961 gaatagagat tgccggttgc gctgaagacc agaaagaggt gaaagaccaa tgccagcacc
     4021 accgtaattt cagcgatgct catggggtag aaaaacagcg tggcggcaaa aaggaaaaag
     4081 gaaagcccga agagaaaagg cgccatcgcc agatttttgg cgattaaata acggtagcgt
     4141 ggcacaggca ggagcatgag ggtttgaaaa gcccttcctt ccgctccgaa aatgttcatc
     4201 aggaaaaaag aaaagttcag aaaaggaagg aagagccaga agtgtggaac ccaccgcatg
     4261 ccggaacctg ttccgcttaa tccgcctccc ttaaaaagga aaatcataaa tacccccatg
     4321 gatgcgggca tgagaaaaag catgcgcata tgagggtgtc gcgcgtagga gcggaaaaaa
     4381 gtgagagtca ggcagctgat gtcggaagca gcgacggcag gaaaaaaagt gtggactgtc
     4441 cgggaaagtc ctttgtcttt cttgctcttc ctgtcacttc cgttgcccgc gggcgattca
     4501 gagtgccccg accggtaata gcgcatggtc gtcgcatatc ccagatacag accggcggca
     4561 ataaacagaa gggaagcaag gaaactgagt acaaccgcat tcggatttgt atgggtcagc
     4621 tgattccaga gaccgtaaat cggcaggagg aacggaatgg tttgatggat gctcagaagt
     4681 cccggcacat aggattccat agcagcgaca tcgaagcgct gttggccatg tgtgagaaaa
     4741 aaacggagga gcagcgtcgg cagctgagcc agtatcacaa ataccacggg cacgattaca
     4801 aaaatgaggc gtctgcgccg cttgttgatc atccacagag caagcacgct gcatagccag
     4861 taagcccagg cattgagagc ggtgataaaa aggagagcga aaagaacaat cggaaacaag
     4921 gagaatatgc cgcagcgggg gatcagcccg gctgccagtg cgacaagagg cggcagcgaa
     4981 aaaaagaaaa gaggggtaca gatgaacatc aggaaattga aaaggaaaat ccagacggga
     5041 cggataggca gtgccagaaa tttatccagg gcgatgccgt cattccgttg cagttcgatg
     5101 agcaccgacc acaggaaaaa gaaaagaaag agcgatgcgg tgccgtccag cagaaaaagc
     5161 aaagccccgg tgccggtgtc ggcggaggtg cggcgggcgg cataaaaaag cgccgcagaa
     5221 agagcggtgg acagcagtcc gcagcccaga aacagcagaa gcagcaggac ttttccgagg
     5281 gggctgatgc gttccagaga accccggatc agcagaaatt taacacgaat aatggcgaaa
     5341 aactggtatg caagcattta ctcatttccc agccaggaga gatttttcga ggccgctgtg
     5401 tgcgctcctg caatctggag aaagatgtgg tccagcccgc cctcttcctg aaagtcttct
     5461 atggtgccct gctttgccag atgcccttca cacagaatgc ctatgtgcgt gcagagcttt
     5521 tccatcactt ccagaatatg cgaagtcaga aaaacagtga cgccccgttt acgaagacgt
     5581 gagagcaggt cgcgcatgat ccgggaggcg acagcgtcca caccctcaaa gggctcgtcc
     5641 agaaagagca gttcaggatc atgaataaca gcggcgcaga gtgccagttt tttgcgcatg
     5701 ccgtgggagt attcgaggat cagtgtgcgg gcggcagtgt gcagactcag cagttcgagc
     5761 agttcgtcgc agcgcttatc cacgactgtg cccggaagcc cgtacatgcg tccgatgaag
     5821 cgcaggtatt cagggccgct cagattttca aaaagacaga gattctccgg cacgacaccg
     5881 atgcgtcgtt tgatttccag actgctccgc tgcagatcaa agcccaggat ccgcgcgctg
     5941 cctgcatcag gtgccaatgt tccggtaagc attttgatgg ttgttgattt tcctgcccca
     6001 ttgggcccca gaaagccgta gaaggagcct ttgggaactc tgaaatgaag ttgatcaacc
     6061 acgtttctgc cgccgtagcg tttggtcagt cccagacttt cgatgcaaat attcatatca
     6121 gtgcgcatct tccaggtttt caaaaagagc tttcagaaca gcgctttccg gacggagggc
     6181 acgcgcctct tccaggcatg ttcttgcggc ggaagttttg tgctgccgga tgtgcacccg
     6241 cccttcattg agccaggtgt tgaagagttc gtctttggtc tttgcctgct tgcgcgcttc
     6301 ctgccaggct tttgccgctt ttttccagtc aggctttgca tcaaattttt ccgcaagata
     6361 gaaattcagt gcatagtcct gcagaatatc aaaagcacgg ggggcgtttt tcactgccct
     6421 ttccgacatt gacatcgcct cttcataaat ctttctttgt gtaattttgc gggtgctcat
     6481 gacggattca gggtgaatca ggtacacctg cacagtattg aacagaaaat cagggttgtc
     6541 gggctcttcc ttgagcgact gccaaaggta ctccaatcct tgcccgtact gccccacatg
     6601 gcaatacagc attcccagat tgttgagtgc tctgcccagg cggggattga gcgcaacagc
     6661 ctgccgccag tgtttttgcg catcgtctgt ttttccctga aaatcataca ggaattccgc
     6721 ataaaaattg tgaagcacag cactgtccgg aaagcggagc agcgcttctt cataggctgc
     6781 ttgtatggcg gaaagacagg cagcagcatg gcttcgtgct tcttcagtgc cgggtctgtt
     6841 gtccggctgc ccttcttttc ctgccgtatc ttccagccgt cgcacctgta tcatcatcag
     6901 cttgtcaaag gtgcgcaccc gtgattccac ttcctcctgc gtcccttcaa gagaggggat
     6961 ttctggagag tccggcgccg cctccgcctg cagcggcagc aaaagagcag ggaacaggag
     7021 cataatggca gacacaaata gtggtaaagg cataagaagc gcatcctcct taaggaaaga
     7081 tatccgcatg tattcactgc agggcactgt gatattatac ataagaagct gatgcaatac
     7141 gctccggtac gccggagccc tcgtggtttt ttatttttga acagcggggg gcacggcttt
     7201 accctgaacc ccgtaaaaat cttccggaag gactccaatt atggctgaat ggactgcact
     7261 cgaacaggaa gtgatggatg acatgttgcg tcgccgtccg gatctgaaat gctgcgaaga
     7321 ggcgatgcgc cagtttaatg gggcattgaa aaactgctac gacaggggag gtactttctt
     7381 cacctgcggc aacggcggct cctgcaccga tgcccagcat attgtggggg agttgtgcaa
     7441 aagctttgag cgtaaacgcc ccttaaccaa tgaaatcagg gaagcgcttt ccagccagcc
     7501 gctgggcaat gaacttgccg acaatctgga gcgggggctg cctgcggtta cattgggtct
     7561 gaacagcgca ttgaagacgg cggtggaaaa tgatatcccg ctccgggata tcgcctttgc
     7621 ccaggagctc aatgccctga tccgacccgg tgatatgctt cttgccatca gcaccagcgg
     7681 caatgcgaag aactgcctta tggcaatgtc ggtggcaaag gcctatggcg gtattgccgc
     7741 agcactgaca gggcccagag acagcaatat ggcacggcat gccgatgtga ttattcaggt
     7801 tcccggagga agcaccaagg aaatacagga agggcatgct gtggtatggc ataccctttg
     7861 ctgtctgatt gaagtggctt attttccgga aatgcgataa cagaacactt gagcatcaat
     7921 acgatataaa gcggagaagg aaatatcatg agtcagaaaa aagcgattaa gattgcctgc
     7981 attggagccg gaaatgtcgg tgccacggcc gcccagttct gtctggaact ggaactcggc
     8041 aatgtcgttc tcacggatat tgtggaaggg ctgccccagg gcaaagcgct ggatatgacc
     8101 caggcgggac ctatccgtgg ctatggatct gtctgcacag gcaccaatga ttatgccgac
     8161 attgccggag cggatcttgt tatcgtaacg gcagggcttc cccgcaaacc cggtatgacc
     8221 cggctggatc tgcttgaaaa aaacggcagc attatcgccg gaatttgtga tgccattaaa
     8281 acgcacgcac ccgattccac tgtaattatt gtgaccaatc ctctggacgt gatggcgtat
     8341 ctcgcctatc agaaactcgg tttccccaaa aaccgcgttc tgggcatggc aggctgtctc
     8401 gacggcgccc gcatgagtgc gtttattgcc atggaactca atgtcagcat gaaaaatata
     8461 aacaccatgg tactcggttc ccacggtgac accatggtgc ccctgcctgc ttataccact
     8521 gtttcaggtg tgccggttac cgagctgatc gcgccggaac gtcttgaagc gattattgcg
     8581 cgcacccgtg acggcggcgg tgagattgtg cgtcttctga aaacgggaag tgccttctac
     8641 gctccggcgg caggtgccgt acgcatggca caggcgattc tccgcaatga gcggcagctg
     8701 ctcccctgtg ccgcactgct ggaaggcgaa tacgggctgt ccgatgtctt tgtcggcgtg
     8761 tcctgcattc ttggacagaa cggcatcgaa agcattcagc cgctggcatt gagggatgag
     8821 gagcaggcgg ctctggaacg gtctgccgct gaagtgcgtg agggcatcga cggactccgg
     8881 tcactggggc tgctgtaaaa acgatccatg attccaacgg ttccgcagcg gaaggcgtca
     8941 gcgtcttccg ctgtttttat ttctggcgtt tcagtcttct gccggcttct cttcttcgta
     9001 tttgatgctg atgtgaaagg ctgccagcac gacagcaacg accgggttaa tgagattgaa
     9061 gaagcagaaa ggcaggtagg caaaggtggc aacacccagc acgcccgccg caaaggcacc
     9121 gcaggtgttc cagggaacga gcacagaggt gagagtgccg ccatcttcca gtgcccggga
     9181 aagattgcgt acatccagtc tgtagtgctg gtatgcgctc cggaacattc tgcccggaaa
     9241 cacgatagcc aggtactggt cggagagaaa gatgttgcag aggatacagg aacctatcgt
     9301 ggacgcaatg agcgtggcaa tgccgcgtac tttgctgagg actgcctgcg caagacgctc
     9361 catcaatcct gtcgcttcca tggcaccgcc aaaaaacatg gctgccagaa tcagagagac
     9421 tgtatcgtac atggccgcca taccaccccg ggacagaagc gtatccgtga gaggctgccc
     9481 gctttccagt ctgaaccctt cgtatgcggt aagcagaatc attttatact gttccagagc
     9541 ggacagactt tctccggcgg gagagagaaa acgctgcggc tgaaaaagga gtgccgctgc
     9601 agcgccgagc agcgtgccga tggtgagggc gggaatagct ttgatgccct ttgccgccat
     9661 aacgattacc agcagcggga tcagcatgag gtaccagcgc acggaaaaac tggcagcaat
     9721 tccgtcgagg atttcggata cagccgcatt gttataggca ccgggtgtat aaaagagact
     9781 gatccccgta aaaataagca gggcgatcag gaaactgggt atggtgctgt agagcatgtg
     9841 gcgaatatga gtgaagagct cgcttcccga tacagcaggc gcaaggttgg tggtgtcgga
     9901 taaaggcgag agtttgtcgc cgaagtaggc gccggaaaca atggcgcccg ccaccagagg
     9961 atccggaaaa gataaagccc tgccaatgcc gagaagggct atacccacgg tacccatggt
    10021 tgaccagctg ctgccgactg ccagcgacac aatggagcag atcaggcagg cggagggcag
    10081 aaaaacagca ggggtaaaaa gctgaatgcc gtagtagatc atggagggga caataccact
    10141 cagtatccac aatccgatga ggcagcccac cgtcagaagg atcagcaccg cctccatggc
    10201 gaggacgatg gattggatgg cttttgcttc tatgtccttg tagctgagcg ccagaagaat
    10261 ccagcccagc gtgaccacca gaacaccgct cagaagcaat gccatctgat tggggcccga
    10321 acttgcatct tcgccgaaac accaggcgtt gagcatgacc agcacgatca gcacggcaca
    10381 gggcagaagc gccacactga aacgtacctt cgcaggttcg gtccggatca taaatgggtg
    10441 ctcctttgct ggcagtgaat caaaactgcc ccgtccgcag cgtcaatgtt taaaagaaaa
    10501 agtggtatcc ggaatctgtt ggcttcccga aaaaatagtc acgcatcctc ctgaccggtt
    10561 tcttccctgc tggcgggcgc ctctgtacta gaggcggagc gaaggttctc tgcttcctgc
    10621 tcccggctga gctcctgatt ttccggatgc cgcgggcagg ttggaatgca ggcaaagcag
    10681 ctgccgtagc agcgctcatc ttcggggtca acggggtgta ccggcatccc ggcgctctcc
    10741 tatagcagtc agccgttcag caagcggaga aatgggcggc gcctgatttt ccggcgcatc
    10801 cagataaaag aaagcaacgg cgcacacatc gtcccggcga tagaagtttg tccagccgta
    10861 agggatgccg ggatcattca aggagcgggg cggttccgat tcgaggaggg gcacaaagcc
    10921 gtcctgggca cctacagaga tgggaaggat ttccactcct ttgcttagca tttctttgac
    10981 atattcctgc atggcgccgc cgatctgctg aatggtgacc cggatatcct gatgaaaaaa
    11041 taccggatcg gggatatgat agcggtacat agtccaatac tgatttacat tgtcgatgag
    11101 cagggaaccc tgataaggat gagcaaattc tccctggaaa tagccggtgc ccacgtaatc
    11161 ttccgtacct gttccgatca gtgtaggcag ggcagtatcg ccgtccagat aaatcttcac
    11221 ttcaccctca ccccaccagc cgagattgtc cgggtgtccg ataatgccga gatgggttcc
    11281 cagataacgc ccttttcctg tggtgcgggg caggatttcg aagtcccggc gcaattctgt
    11341 ataacgttcg cggtgccaga cagcgtgaaa ataaaggggt ttttcttcaa acttcatctc
    11401 ggaatagttg atgtcgtaaa agaactgcgg cagatcttca tcggattcgt tggtaaaggt
    11461 aatgcgtgca tgggtctgaa aaggcatagg gatggtgaag ttgaaagaac gtccttcagg
    11521 gctgctgaag aggtcgttgt ggaatggccg gacttctccc agaatcgcac cgaaaaaatc
    11581 gccgaaaggt acttccacag caggtttttc cgcctgatcc cagtacatac gcaggacaaa
    11641 agaacggaga tcgcgcgggc tgcgcttggg aagcgtgcac cacatacggt gaataagccc
    11701 gctgcccgat acatccatca atactttttc ttctcctgct ttgatgacct gaaaagcggc
    11761 acccttggct ccgccgttgg cagtaccgcc cgagcctttt gccgcatcgg gattctcaaa
    11821 actgacccag tggctccgga cattgtcttc catccgataa agcggcgcac cttcggcgta
    11881 aagtccggat gaagcggtca gcgtaaaaag gaagagagca gccgacaggg gcgcaatata
    11941 tgttttcatc gcagttttgt ctccattcac agaggcttat gccccgatgt catgtcagag
    12001 tgtgacgacc cgggcttcaa aaggggacac ttctgtatca ataagagtat aacggatttg
    12061 gccttcccgg gagagcaggg tccccgctga ggcgaaaagc gttccgtcag catcaacgga
    12121 aaaaggctcg gtcgaaccgc cgctgataat gattcgggcg gtgcacgctt cacgctggcg
    12181 ctgaaatttc aggaagctcg tgtcttttgt gagtgtttct gaagggctgt ttacggcacc
    12241 gtggcagagc agccattcca cgccttcccg gatgaagctc cgcttacgag gcagcccatt
    12301 caggaattcg atggcgctgc tgtcgagcag cgcatagctt ttctccaatt cagccgcctc
    12361 gggaagccgc ttcgcttttg ctttacgcga gaccagtgca cggtcaagtc gtccctgcac
    12421 tgccgttacg gcacgttccc gcaaaagctt cacacaggcg gacgcaccct cgggtgcaag
    12481 gcaaatattt ccggtatgca cgacctcgcg gatgccttcc tcttccagat gatccagaac
    12541 ggtctgcaat gaacaggcgt tgctgagaat gtcgccgatg actgctagaa acataataat
    12601 ggtgctcgtt tcgggtagga aaatctcttg tatttttctg caaagcagcg ttttattgcg
    12661 tatgcctgcc ttgcccttag agtattgaaa gcgcttttaa gttgtcaaat agtctgtatg
    12721 aacttgtttg agtcgccctg ttcggataca cttttgcagt gtgttaaaca agggcccgct
    12781 gcagtgccat aacacagact tgacactgct tttaaaatta tgcacattat acctgtaaat
    12841 catagcgctt ttgttgtata attgaatcag cccctggcat ttgcccggag ttgagaggaa
    12901 tgtactgttt ttcgccgttt cagcgcaagg tgcactgcaa cgatgtttta ggatcaccaa
    12961 actttttcga aggaggaaac tcacccatgg ccattaaaaa agccaaagcc accgacaaag
    13021 cattgacaaa agccgcaaca ctgaccgtcc ttgccgaaga gaccggactg accaagaaag
    13081 acgtcgcgaa tcttctcgac gttctggcga atcttgctta tgcgcaggcc cccgccggct
    13141 tcacgcttcc cgggctcggc aaactgaaac tcgtccagcg cgctgcccgc acaggccgca
    13201 accctgcaac gggtgaagcg atcaagattc ctgcgaaaaa agtgctgaag ttcacgatcg
    13261 ccaaagctgc caaagacgct gtttgcccgg ttaaaaaata accgcctttt tctgtgtttg
    13321 ctctgcccgg aaccgtgctg ttccgggctt ttgtttttta ctgcgccgct gtgatacaac
    13381 acgggacagg ggatagtagt cgtcataaaa ggcttttcaa tcctgaaagg agttgtaggc
    13441 atgtacagaa aatataccgg gctggcaatt atcgccgccg tactttttgc cggctgcacc
    13501 catctgcccg ggcttaccag tctgccggcg gcgccggaac cgctcctgat cgcagagaat
    13561 ggtgtcacac ccatgaagat tgtggtcagc gaatctgcgc cggaaccaac ccgccatgcg
    13621 gcgcaggaac tgaggcattt cctcaaagag attacaggcg ccgacctgca ggtggtggat
    13681 gacacaaccc ctgcagggga aagcgaaata tggttgggaa acagccggcg ctacagcgac
    13741 aacaacattc tggtggatta taaagttttc ggcaatgaag ggtaccttat ccggacgcgg
    13801 ggcaggaatc tgctgatcac cggaggagaa ccgcgcgggg tactctatgg tgtttacggg
    13861 ctgctggaag accatctggg ctgccggtgg ttcactccgg aaatttcctc catccccaaa
    13921 gcaaatgtac tggccgtact gcctattgac gaagtgcgcg tgcctgccct cgaatatagg
    13981 gagccttttg tttatgactg ctgggacgga gactgggcag cccggaaccg gatgaacagt
    14041 cagtccgcac ggttgggttc gcagcacggc ggcaaagtcc agtaccaggg ctttgtccac
    14101 acctttgatc tgctggtgcc gcctaaggaa tattttgata cgcaccccga atatttttca
    14161 ctggttggcg ggaagcggct gaaagagcgc acacaacttt gctgcaccaa tgaggaggtc
    14221 attcatctgg ttacggaaaa agtgcgtcgg ctcatggcgg agcatcctga ggcgacggtt
    14281 ttttctgtct cacaaaatga ctggtacaat tattgcgaat gcgagcactg cacggcactg
    14341 gcagaggcgg aaggcagcca gatagcacct gtgctgcatc tggtgaatca tgtcgcccgt
    14401 gcggtcaaag atgaatttcc cgataaactg atcgacacgc ttgcatacca gtatacacgc
    14461 aaggcgccga aaaccatgcg ccccgagccc aatgtgatca ttcgtctctg cagcatcgag
    14521 tgcgattttt cacatccttt cgaagatcgg ctcacaccgg aaaaccgcgc cttctgtgac
    14581 gatctggaga actggagccg gattgccaac cggctgtgga tctggaatta caacacgtcc
    14641 ttccatgaat acctggtacc ctttcccaac ctacgggtga gggggcccaa tattcgctat
    14701 ctcatcgcca ataacgtccg gggaattttt gagcaggacg tgtacaatac gccgcatggt
    14761 gaattcagtg cgctgagcgg ttatctcggc gccaaattgc tgtggaaccc ggaatacgat
    14821 acagatctcg ccatcaatga atttctgaat ggtgtctacg gcgccgccgc cacacccata
    14881 cgcctctacc ttgatctgat ccatgatgcg gtaaaagagc cttcaaccag catgaatatc
    14941 tggatcagca ctgatgagcc tttcatcacc gatgcccttc ttgaacgagc ggacgcactt
    15001 tttgatattg cggaaaaggc tgtgccggat gatccggtgg cgctggaaca ggtgcgcata
    15061 gcccggctgt ccgtggacta caccattctg tcgcgcaccc agcgcatgcc cgggaaagcg
    15121 tatgtgctgg atcatgcccg aggcaccgta aaggcgagtc cggagtttaa agcacgggca
    15181 gcacgcttct ttgatgtggc ggagcgttgc ggctttaccc agctttggga agtgggcggt
    15241 cttctctctg aatacaaaaa agagatttat gccctttttg aagagcatgc ggccggtacg
    15301 cttgctgcac cgtctgaagt tccaccgctg aagcccggtg cttttttccg ttactacaaa
    15361 atgtactgcg aggcactgcc cgatttctcc gccctcagcc ccacggaaga aggggtcgta
    15421 tccgcactgg atctggagcc cggaaagaaa tatcagccca cagcggatgc ctacctgtat
    15481 accggatata ttgaaatccc gaaaaacggt gtgtatgcct tcagtgtaaa ttccaacgac
    15541 ggcagccgcc tgaaaattaa tggcgaggtt attgtggata acgacggtct gcacaaggcg
    15601 acttccgtaa tgggattccg attgatggag aagggctttt atccctttga gctcgcctat
    15661 tttgatgcag gcggcgggtc tgcgctggag gtgcagtatg ccggacccgg cattcccttc
    15721 cagcctcttg ccggtgcgat tctttatcac taattgatgc agccatttct ccgttcacgg
    15781 aaggcatgca aggagaataa acgaatgaat gatcgtttcg gctggctcgg ttttactaca
    15841 gggctgcttt tcgtgtgctt ttttgcgctg ctttttcacc ttgggtttca gaaagcgtcg
    15901 ctggacaagg agcggaagcc ttctgtttcc ggaggaaaca ctgtagcgaa aacagcaggt
    15961 gcaacgttac aggctattga tggaccgcct acccaggaaa aaattgatgc ccttttgcgc
    16021 ggagacagcc cggatgctgc aaacagggaa actgctacgc cggagcgcaa aaaagaacag
    16081 ctcccgcccg ctgaagcagt gactgttcgc cgctcgttgc ctgcccgaaa tgtcgtgcag
    16141 ggcggaatag tggatacgct gctccatgtc acgaaaagag acccgttgcc gcccgaggca
    16201 atcatcattc gtgaatcgct tcctgatgga tggtcactga aggaggtggc tccgggtgta
    16261 ctgccgccgg atcttgtacc cgagcccggc actgagaagg atctggagtt tcgctggctg
    16321 caccctgccc gctttcctat tgtgctcaga tacagtgtgc aggtgcctgc tgatgcagcg
    16381 gaaaaagcgg aatggaacgg ggctgttacc tggatttatg acaatgtgcc caacaccagt
    16441 gtgcttcctg taacggaact gcatatatca gcggagaaag ggggacaatg aacagtgacc
    16501 tgatcattcc gccggcacgg gcacattccg gcgcttccgt gagcatgcag gggaaggtcg
    16561 tgctggttcg ctgtgaaggc tacgaggaag cagccgttta tagtgcggtg caacgcggtg
    16621 ttgatttact gggcgggata tcccgctttt ttcaggcagg agagaccctg ctcctgaaac
    16681 ccaatgtgtt gagcggcaaa tcccctgacc gtgccattac tacccatccg tccgtgtttc
    16741 aggcggtggc acgcctcttt caggaagggg gcgcatcttt aacttacggt gactctcccg
    16801 gtttcggcaa agtggagacc gtggtgcagc gtgcgaagct aagctccgtt gctgaaaaac
    16861 tcggcatacc gctggcagat ttttcagaag ggcgcaccgt gtcatttcct gagggagcca
    16921 taagcaagca gttcacgatc gctgccggag ttctggcggc ggacggtatc atttcgctgc
    16981 cgaaactcaa aacgcatgcg ctgactcgcc tcaccggcgc cgtgaaaaac cagttcggtt
    17041 gtattccggg gctgcgcaaa ggcgaattcc atgcacgaat gcagcgcatc gatcagtttg
    17101 ctgccatgct ggtcgatctg acccggatgc tttcacctcg cctttatgtt atggacgggg
    17161 ttgtcgccat ggaaggaaac gggccgggga atggtacgcc ccgaccgctc aacgttcttc
    17221 tgttttccac agatcccgtg gcgctcgatg cggcagtgag ccgtattgtc tgtctggatc
    17281 cggcgctggt gcccaccatt gtaaaagggc agga
//
LOCUS       DAOPWB010000067        17292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3354_length_17292_cov_5.203690, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000067 DAOPWB010000000
VERSION     DAOPWB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17292
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3354_length_17292_cov_5.203690"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="PKN92_07650"
     CDS             <1..1139
                     /locus_tag="PKN92_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ48704.1"
                     /translation="NLTLDIDLQRKCDALLQGEQGSIVVLDADTGAVLALASAPGYDP
                     SVFVNRGATEERLALLSGQKPNRMTHRAFREVYPPGSVFKIMLAAAGLEENLINAGTR
                     FHCSGSYAIDGKGRRWNCWNRHGHGSLNVVESLAFSCDVFFYSLGRRLEVDRIVAYSK
                     LMGLGVPTGIDIAGESAGLIPGREWKAAMHADKPAWEKQWYPGETVNLSIGQGSCTTT
                     PLQVTVMMACIVNGGYRVQPFLRQEAGPKRSEKLFSDTTIKTVTEGLRMCVERKKPAP
                     TGTGTRAYIDGMDIIGKTGTAQVASLSFLSKYKKTEDIPYPLRHHAWFTAGVLDRTPR
                     IAVTVLIEHGHAGGSVAAPVARDVIEYFYNHAATKPSYTITAGN"
     gene            1145..2386
                     /gene="rodA"
                     /locus_tag="PKN92_07655"
     CDS             1145..2386
                     /gene="rodA"
                     /locus_tag="PKN92_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNZ48705.1"
                     /translation="MPSLRRFISHQNVSVRLLNFTNVMRVDIWLILNVLALAVIGWIL
                     MYSVARSASTVYLYRMILFFCSGVVVTIFFVSIDYRFLVNWAPLFYLGSIALLLVVLF
                     FGTEAKGSERWINLGLFRLQPSEFSKIIMIFFLTWYLTKIGEKIRKLGWFVVTFFLVG
                     IPLALILKQPNLGTAASLAPLVFAMLYVAGCRKVHLVLVLLLGSMLVPVLWLEMKDFD
                     PSVEKETQTTLGLKHYQKKRIYAFLHPEYDYRGTGWHTYQSKITIGSGGISGKGYLRG
                     TQTKLKYLPEHHTDFIFSLLAEEFGFVGGVMVIALFAIMLFRGLMFARDAYDMQGTLL
                     AVGVVTVLAFHIFVNIAITTGLLPVTGIPLPFLSYGGSFYMTVMAGIGILLNVPLRKK
                     EVEQQNMPTSIANVEVYRRKG"
     gene            2408..4063
                     /locus_tag="PKN92_07660"
     CDS             2408..4063
                     /locus_tag="PKN92_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNZ48706.1"
                     /translation="MRIVLMFCLCCSAVCASVTGHWPLEKDVNDSSGGALHPHNQGVV
                     FDTPAPDGTQAARFNGIDAYLEIPSHLLSDLLQKEFTVAMYVYTEATLDDAVGDLLSQ
                     FDPETRTGFTLGVADYSGVCNSSPNHRNLFCGIDQGTEPVWEFCGRPGKAIFIFGFAV
                     LEGKLYAATCEPGSGDAGHVYCYDGGTRWIDCGSPDASNAVMALTVHEGNLFAGAGWY
                     DTTGSSLEASANEERSGKVYRYLGGTSWEFCGQLANEETGEAGTLGGLAVYQGALHAT
                     TLKKEGFGLYRYEGGTTWRYLGNPGRRVLNPSVFNGGLYMVSYDAPGGPFLFDGKEWS
                     YRGASLDPPIHQDYSFAVYGGQLYLSTWPLAYVYRLEGDTWLAAGRTGEELETMGMMV
                     YNGKLYTGTLPSSCVYRYDGKDIWTKVSDQLDTAEGRYRRTWSMALYDGKLFCGTLPS
                     GNVYSMSTGACTSWNRSLPSGWRHIAATRQGAALKLYVDGDCVASSENASASALQSSP
                     DVSLLIGAGPGDYFNGWMRDLHLYDTCLSPEEIKELATEGKRQ"
     gene            complement(4105..5583)
                     /gene="glpK"
                     /locus_tag="PKN92_07665"
     CDS             complement(4105..5583)
                     /gene="glpK"
                     /locus_tag="PKN92_07665"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNZ48707.1"
                     /translation="MKYVLALDQGTTSSRAIIFDQTGTIRASAQQEFEQIFPEPGLVE
                     HDPSAIWTSQLKVAREALLKANLKASEITAVGIANQRETTLVWDRKSGTPLYNAIVWQ
                     DRRTSAFCETLRSQGYNDLFREKTGLLLDPYFSGTKIKWILDHVEGASEKARRGELAF
                     GTVDTWLLWNLSGGRIHMTDASNASRTLLYNLHTGTWDNELLELLAIPASLMPEIVDS
                     SRIYGYMQEDLLGASVPIAGMVGDQQAALFGQLCTRPGMAKNTYGTGCFMLMNTGSQA
                     VRPETQLLTTVAWQLKGELQYALEGSVFVAGAVVQWLRDQLGIISSSSKIETLARTVP
                     DNGGVYFVPAFTGLGAPWWDPHARGTISGLTRGATAGHLARAALEGIAFQVEDVLDAM
                     SSASGVAVQELRVDGGAAANNLLMQFQANISNTPIIRPKCLETTAAGAAYLAGLATGV
                     WDSVDALASQWEMDSTFAPAFSEDERSAFKTGWHRALQRAKS"
     gene            complement(5759..6613)
                     /locus_tag="PKN92_07670"
     CDS             complement(5759..6613)
                     /locus_tag="PKN92_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ48708.1"
                     /translation="MKTRLFLPFILFLLLLTGTPFASAQFETPVIKEWVVKETNDNGE
                     VEEVEYMSLDGVLVHETDPALAPQPPVVQPGAVTGMPPSDAIVLFNGKNTDGWSADDG
                     SPSQWIVKDGALQPVEDSGVIRTVQEFGSCQLHVEWATPANVEGDGQGRGNSGVFLMG
                     QYEIQILDSYDNTTYPDGQAGALYGRSAPLVNACRKPGEWQSYDIIFHRPVFDASGNV
                     VKRATFTILHNGVLIQDHLELSGGTLWKSKHSVVPYEAHGDKGPITLQDHGNPVSFRN
                     IWVRELAD"
     gene            6910..>7302
                     /locus_tag="PKN92_07675"
     CDS             6910..>7302
                     /locus_tag="PKN92_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48709.1"
                     /translation="MSVKAIAERAMEQGQGVLRLTPTWVPRVFCVPGRRIKLHPHDYY
                     ALGGERGGIDERWFSSTTPADNGPLTSKNEGLSHIAFEDGGKTELFLLRDAIEELKDA
                     IIGKLWAKYQAWPMYSKFFDNQGALPFHI"
     assembly_gap    7303..7312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7313..8148
                     /locus_tag="PKN92_07680"
     CDS             <7313..8148
                     /locus_tag="PKN92_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48710.1"
                     /translation="IHHDDDHAARVGARGKPEAYYFPPQLNNHGGDFPYTFFGLAPGV
                     TKEEVLDALRAFTKGENRITDLSAAYVLTPGTGWNVPPGVLHAPGSLCTYEPQKASDV
                     FAMYESVVHDQVLSEDLLWKDSPEEEIGNHEYLLEVIDWEQNTDPLFRQKHFMKPVPV
                     DDPAEMRAAGYQENWICYLSKEFSAKELTVLPGATVTIKDGGAYGLYVVQGHGTLGIW
                     DIESPALIRYGQLTYDEYFVSEAAAREGVTITNPSKSDPIVMLKHFGPDAHTNMPVPL
                     D"
     gene            8171..9223
                     /locus_tag="PKN92_07685"
     CDS             8171..9223
                     /locus_tag="PKN92_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNZ48711.1"
                     /translation="MTNQSNNFPKLHNAAWPGLVGKGPGSEPPIDLDTMLNLTAAAEV
                     DGIKFDGIDLFLADPHISIDIDDDGIKALADKIHGLGLQVGSLVAPVWVDAGGGSAMD
                     EEKGRPRFLEQVRKACRIGAKLRELGIRPDGAVRIDSAVSVESWAQDPEGNQKKIART
                     FSKAADIAADHGEILVAEGEVCWGGMHSWRRMSQLLEMVDRKGQFGFQADMAHTLLYL
                     LGANAPEDALLPPDFQWNDESAFDLAYKSLCAALRPWTLDFHVAQNDATVFGSGTHDK
                     TGRHCLPEDPNGKLDIVKYAGFWLRDNQGNLKKKIRHLCWDGCMFPNETLLNPATWNS
                     ILSAMIAVRNAHGWQE"
     gene            9270..10409
                     /locus_tag="PKN92_07690"
     CDS             9270..10409
                     /locus_tag="PKN92_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48712.1"
                     /translation="MTQIRLGMIGYGFMGRAHTNAFKRVPDFFPELKYKPVLQAACGR
                     NQDKVNAFAAQWGFASTETDWRKLLERDDIDAVDICAPNDRHKEIALAAIAQGKMVLC
                     EKPLAMNTADGIEMAEAAEKAGVANTVWYNYRRIPAVMLAKHIVESGKLGRIYHYRAN
                     FLQDWCISEDVPQGGASNWRLNIAEAGSGVTGDLLAHCIDTALWLNGSIDKVTAMTET
                     FVKQRIHVETGQLAPVGIDDACAFLCRFSNGSLGLFESSRYARGHKALYTFEINGANA
                     SIRWDLHDLHRLEYFDHGDESALRGWRSIHVSDHGGDHPYMGRWWVPGLQIGYEHSFV
                     HQAADFMESLEKGQPCPPTFRDAVETQKVCEAVLNSAASEIWEKI"
     gene            10495..13308
                     /locus_tag="PKN92_07695"
     CDS             10495..13308
                     /locus_tag="PKN92_07695"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ48713.1"
                     /translation="MRYCMFVMCCAFSCAFSLSAAYGQEDAPDLAFDSAVAAIGQDAT
                     AKGALEEECLSTLKSSDEWADLYRAVRILSVIGTEKSVPVLSEMLVKLEQSHLARIAL
                     ELIPGEEASAALRDALTKAPEKEAIGIASSLGVRKDSGAVSVLADLAAGDQEGLAQAA
                     LAALGHISGKKATEVLKSHILDQNRERAAVAAEALLVALEEAESDTETTAGVYESLLE
                     KSWPDRIRAGAFMRILEEDTEKAVSHILEMVYSNDLFLRSVALASAISLKSGDLVGQL
                     VEELPSLAEDVQARLIESLGKREEGIAEKTLHQFLHSPSEAVRIEAAKALGNNGTSAS
                     IAPLAEMIARAEGRTEKNVAAETLRRLKGKDVDAALVDYLSNAPLAARPEIIEVLAQR
                     DVKEAVITLIAHTRIDGCAPAAFQALAKLADVDQVPVLLGLLTVLDGDKGRPEAENAA
                     VALCRKAQEKDAGIEIRSAVYGDWPDGACADVTDKVKQAVQDGTFSIIAQNADYGDPA
                     PNIVKKLHLEYLERGVVKQKTVSEGATMNLASDMLPLQVFEMIKGNLLATTDANAQVS
                     FLKVLSRLGDPTTFSLVQEYLEHSDPRVVDGAVRAVAAWPTVQALDTLVDLTQGSGEA
                     AQQSIAFRGAVRLLRLGEISQEKTLECYKSLITAAKNTAERKLLLSGLGETGNIEAIS
                     LITALAKTEDLQAEADAALGRIKEKVGEETFNKAMNPGGAAPLITGKFVSIFDGKTLD
                     GWSGNPQYCRVEDGCIVLETKNADELEHNTFLSWNVEELEDFEIMFQYRIDSEWANSG
                     IQIRSEYFSDEQFRVRGYQADISNEDWVTGLCYEEGGRQFLAYRGEKSVVTDDGVQSE
                     RFADQKALGDYIYPHDWNQYHVIVKGDTFVSRINGHTMHEVTDHSSVAKRRGIIAFQI
                     HKGPPMKVLFKDVKVKKLEPASN"
     gene            13351..14616
                     /locus_tag="PKN92_07700"
     CDS             13351..14616
                     /locus_tag="PKN92_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48714.1"
                     /translation="MKSVKTLTRRAFLRQSTMAAGALSVSPLVMSRAFGDANPSDKIT
                     VGAIGIAGRGRYILNAFLQYSDVKVVAVCDVFADRRNRAKQMVDEHYGNDDCAAYIDA
                     RELIARPDIDAVMIATGDNNHAPLSILAARAGKDIYCEKPMSVTIAESRAVSDAVRHN
                     GRIFQCGTQRRNVGNFVLAVELARSGALGEIQTVYAEKAWPDSTIYYGVLPEEPLPPY
                     EEMAWDLWLGPAAWRPYNLKYTTGFWRRHGDFSGSSIAEWGSHTVDLCQWALNADDTS
                     PVHYRIMNELGDIEARYANGAKLVITKGLRFGSCPVRFEGTEGWVETGDDNTIETSPA
                     SLASDRHFSGGYPADNHVREFLNSVKSRQPTKSTAETSHRSITACHCANIAIKLKREV
                     TWDPVKEAFVNDDEANRLRSRAYREPWRM"
     gene            complement(14700..15986)
                     /locus_tag="PKN92_07705"
     CDS             complement(14700..15986)
                     /locus_tag="PKN92_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48715.1"
                     /translation="MKSENPTALPPLTRRSFLHHAAVTGTIAAGLSAGLHRRGYSANE
                     RLGVGVIGAGNRGNSHLDALKHIQDEDKSIELRAVCDVYRPRMTRTAERFGIKGYMSH
                     LELLEDPSVDIVTIATPDHIHGYQALDALHAGKGIYCEKPVTHWRQFELTKQLAKEVA
                     ASRGVFMCGTQGMSDTAWRQIGQLVREGLIGQPIHAECGYFRVGDWGEAGMPIDDPNA
                     RPGADLNWEAFLGDAPQRAFDVSRFFRWRMYEDYAGGPCTDLFPHILTPVMHMMNAGL
                     PSVAVATGGKFRYSEREVPDTFNMLVDFPENFTIAVLGTQGNDFQASENRGSASRVPV
                     IRGWDGALTVSNDKIIFTPAHGSNKEKQLFDIDRGENLLHFFREFIGCFRNSNPNTIS
                     NMELAYRVQTALIMSYLSLRESKVVKFDRQTESILL"
     gene            complement(16060..16707)
                     /locus_tag="PKN92_07710"
     CDS             complement(16060..16707)
                     /locus_tag="PKN92_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNZ48716.1"
                     /translation="MAYRQHVECLQKGVDAWNAWRKETRHSRPDLSGLNLSGLDLHRL
                     DLAFTNLSHANLSKVDLRGTDLTQCNFFGANFLGAKLAGAILNSAELSNADLLNADLR
                     DASFENATLNNVVLHEYNFHGMNFKNASFFESDLTKCDFSTADLSGAVLSSAKLDLVN
                     FYMTNLQNATISQCSVRMAVFKNALLEGIVVHETNLLEANLSLKEKMKLKLHGKL"
BASE COUNT         4216 a         4095 c         4622 g         4349 t
ORIGIN      
        1 tcaatttaac tctggacata gatctgcagc gcaagtgcga cgcgctattg cagggggaac
       61 agggcagtat tgtagtgctg gatgcggata ccggtgccgt tctggcgctt gcgagcgctc
      121 ccggttatga tccaagcgtc tttgtaaaca gaggcgcaac cgaagaaaga cttgcacttt
      181 tatccggaca aaaacccaac aggatgaccc atcgcgcttt cagagaagta tatcctccgg
      241 ggtctgtgtt caagattatg cttgcggctg ccgggctgga agaaaatctg atcaatgcag
      301 gtacgcgttt tcattgcagc gggagctatg ccattgacgg aaaaggccgt cgttggaatt
      361 gctggaaccg ccacgggcat gggtcgctca atgttgtgga gtccctggcc ttttcctgtg
      421 acgtcttttt ttattcactg ggcagacgac ttgaagtgga tcggattgtt gcatattcca
      481 agttgatggg actgggtgtg ccgaccggta tcgatatcgc aggagaatct gccggactca
      541 tcccgggaag agagtggaaa gcagctatgc acgcagacaa gcctgcctgg gaaaaacagt
      601 ggtatccagg agagaccgtt aatctcagca tagggcaggg cagctgtacc accacaccgc
      661 tgcaggttac agtgatgatg gcgtgcattg tgaacggcgg ttatcgtgta cagccttttc
      721 tgcgccagga agcgggaccg aagcgctcgg aaaaactctt tagcgatact acgatcaaga
      781 cggtaaccga aggtttacgg atgtgcgtgg agcgtaaaaa acctgctcca acaggcactg
      841 gtacccgggc ttatattgac ggtatggaca tcattggaaa aacaggaacg gcgcaagtgg
      901 cttccctgtc ttttttgtca aaatacaaga aaaccgaaga tatcccctat cctttacggc
      961 accatgcctg gtttacagca ggggtgctgg atagaacccc ccgtatcgct gttactgttc
     1021 ttattgaaca tggacatgcg ggtggctcgg ttgccgcacc agttgcccgg gatgttatag
     1081 agtattttta taatcatgca gcgaccaagc cctcttacac gataacagca ggaaactagc
     1141 tctcatgcct tcattgcgaa gatttataag tcatcagaat gtgagtgtca ggctgctgaa
     1201 ctttacaaat gtgatgcgtg ttgatatctg gcttattttg aatgtgcttg cgctggcggt
     1261 gatcggatgg atccttatgt acagcgttgc ccgcagtgcc agcactgttt atctttaccg
     1321 tatgatcctc tttttctgca gcggcgttgt tgtaaccatc ttttttgtaa gcatcgatta
     1381 tcgctttctg gtcaactggg cgccgttgtt ttatcttgga tcaatagcgc ttctgctcgt
     1441 agtgcttttt ttcggtaccg aagccaaagg aagtgaacgc tggatcaatc tggggctttt
     1501 cagactgcag ccgtctgaat tttcaaaaat tatcatgatt ttctttctga cctggtatct
     1561 tacaaaaatc ggagagaaaa tcaggaaact ggggtggttt gtcgttactt tttttcttgt
     1621 gggtattccc ttggcactca tccttaagca gcctaatctg ggaactgccg cttctctggc
     1681 acctcttgta tttgcaatgc tttacgtggc aggatgccga aaagtgcatt tggttttagt
     1741 ccttttgctg ggatccatgc tcgttccggt actgtggttg gaaatgaaag actttgatcc
     1801 gtcggttgaa aaagaaacac agacgacgct gggtttgaag cattatcaga aaaaacgtat
     1861 ttacgcattt ctgcatcccg aatacgatta ccgtggtaca ggctggcata cgtaccaaag
     1921 caaaataacc atcggaagtg gcggtatttc cggaaaagga tatttgcgtg gcacgcagac
     1981 taaactcaaa tatctgcccg agcatcatac tgactttatt ttttctttgc tggcggaaga
     2041 atttgggttt gttggcggag tgatggttat tgctcttttt gcaattatgc tttttcgcgg
     2101 cctgatgttt gcgcgggacg catacgacat gcagggtacg ctgctggctg ttggtgtggt
     2161 gacggtgctc gcttttcata tttttgtaaa cattgctatc acaaccgggc ttttaccggt
     2221 caccggaatc cctttaccct ttctcagtta tggtggcagt ttttacatga cggttatggc
     2281 aggtataggg atactgctca atgttccctt gcgcaaaaaa gaagtggaac agcagaatat
     2341 gcccacttcc atcgcaaatg tcgaagtcta ccggcgtaaa gggtaatcac agattcagga
     2401 ggattgtatg cgtattgttc tgatgttctg cctttgctgc tccgcagttt gcgcgtctgt
     2461 gaccggccac tggcctcttg aaaaagatgt taacgactct tccggcggag cactgcatcc
     2521 gcataatcag ggtgtcgtct tcgacactcc ggctccggat ggaacacaag ccgcccgttt
     2581 taacggtatt gatgcttatc ttgagattcc ctcccatctt cttagcgatc tgctgcagaa
     2641 agagttcacc gtcgccatgt acgtatatac tgaagcgact cttgacgatg ccgtcggtga
     2701 tttgctcagc caatttgatc ctgagacacg aaccggcttt acactcggcg ttgctgatta
     2761 ttccggagtc tgtaattcct cgcccaatca caggaatctt ttctgtggga tcgatcaggg
     2821 gacggaacct gtctgggaat tttgcggaag acccggaaaa gcgattttta tttttggctt
     2881 tgccgtgctt gaaggtaaat tgtatgctgc cacctgtgaa ccgggttcag gagatgcagg
     2941 acacgtgtat tgttacgatg gtggcacaag atggattgat tgcggatctc cggatgcaag
     3001 caacgcagta atggcactca ccgtccatga aggaaatctc tttgcaggtg caggatggta
     3061 tgacacgacc ggctcttcac tggaggcatc tgccaatgaa gagagaagcg gaaaggtcta
     3121 ccgatatctg ggcggcactt cctgggagtt ctgcggacag cttgccaatg aggaaacggg
     3181 tgaagcgggt acgctgggcg gtctggctgt gtatcagggt gcgttgcatg caaccacatt
     3241 aaaaaaagaa gggtttggtc tgtatcggta tgagggaggc acaacatggc gttatttagg
     3301 caatccggga agacgcgtcc tgaatccttc cgtttttaac ggtggactct acatggtgtc
     3361 ttatgatgcg ccgggcggtc cctttctgtt tgacggaaag gagtggtctt atcggggcgc
     3421 atcgctggat ccgcctattc atcaggatta ttcttttgca gtgtatggcg gacagctgta
     3481 cctttccacc tggccactgg cttatgtcta tcgtctggaa ggggacacct ggcttgctgc
     3541 cgggcgtaca ggggaagagc ttgaaaccat gggcatgatg gtttataacg ggaaactgta
     3601 cacaggaacc cttccaagtt cctgcgtgta tcgctatgac ggcaaggata tctggacaaa
     3661 agtgagtgat cagctggaca cggctgaagg cagatacagg cgcacctggt ccatggctct
     3721 ttatgacgga aagcttttct gcggtacact gccgtccgga aatgtctata gtatgagcac
     3781 gggcgcctgc acttcctgga accggtcgct gccttccggc tggcggcata ttgccgcaac
     3841 ccgtcagggc gctgctctga aactttatgt ggacggtgat tgcgtcgctt ccagtgaaaa
     3901 cgcatcggcc tctgcactgc agagcagtcc ggatgtctct ttactcatag gtgcaggtcc
     3961 cggcgattat tttaacgggt ggatgcgtga tctgcatctt tatgacacct gcctttcgcc
     4021 tgaagagata aaggaacttg ccacagaagg gaaaaggcaa taatttcttt tccggtacaa
     4081 ggacgcagga gacgggatat tttcttagct ttttgcgcgt tgaagggcgc gatgccatcc
     4141 tgtcttaaaa gcagaacgtt catcttctga aaacgccgga gcaaaagtgc tgtccatttc
     4201 ccattgtgaa gcgagtgcat cgactgaatc ccacacaccc gttgcgagcc ctgcaagata
     4261 agccgcacct gcagcggtgg tctccaggca tttaggacga ataataggcg tgtttgaaat
     4321 gttcgcctga aattgcatga gcagattatt ggcggcagcg ccgccgtcca cacgcagctc
     4381 ctgcacagca acaccggatg cactgctcat ggcgtcaagc acatcttcca cctgaaaagc
     4441 gattccttcc agagctgccc gggcaagatg tcctgcggtg gctccccggg tgagaccgct
     4501 tatggtgccc cgtgcgtgag gatcccacca gggggcaccg agtccggtaa atgcaggcac
     4561 aaaatagaca ccgccattgt ccggcaccgt tcgggcaagg gtctcgattt tggaagaaga
     4621 tgaaatgatg cccagttggt cgcgcaacca ttgtacaacg gctcctgcca cgaatacgct
     4681 cccttccaac gcatactgaa gctcgccttt cagctgccac gcaacagtcg taagcaactg
     4741 cgtttcaggg cggacagcct gagatcctgt attcatcagc ataaagcagc cggtgccgta
     4801 ggtgtttttc gccatgcccg gccgggtgca cagttgaccg aaaagagctg cctgctggtc
     4861 tcccaccata cccgcaatgg ggaccgatgc gcccagaaga tcttcctgca tatatccgta
     4921 aatgcggctt gagtctacga tttccggcat caggcttgcc gggattgcga gcagctccag
     4981 cagttcattg tcccaggtgc cggtgtgcag attatacaaa agggttctgg atgcattgga
     5041 agcgtctgtc atatggatcc gtccgccact cagattccat aagagccagg tgtccactgt
     5101 accgaaggca agttctcccc gccgggcttt ttcagaggct ccctcgacat gatcaagaat
     5161 ccattttatt ttggtgcctg aaaaataagg atccagcaac agccctgttt tttctctgaa
     5221 aaggtcgtta tatccttgac tgcgtagcgt ttcgcagaat gccgacgtgc gtcgatcctg
     5281 ccacacgatg gcgttgtaaa ggggtgttcc tgatttgcga tcccatacca gagtcgtttc
     5341 cctttgattg gctatcccga ctgcagtaat ttccgatgct ttaaggtttg ccttcaaaag
     5401 tgcttcgcgc gccactttta gctgcgatgt ccaaattgct gaagggtcat gttcaacgag
     5461 tccgggctcg ggaaaaattt gctcaaactc ctgttgagca ctggcccgta ttgtccctgt
     5521 ctgatcaaaa ataatggccc gggaactggt tgtgccctga tctaatgcaa gtacatattt
     5581 catacgttta ttcctgcttt caacgggtga gcccggttcg tgaacagatt taacgcacgc
     5641 ctatagtgta ccccatgagc actgaacagg caagagaggg cataaaaaag gcagaccata
     5701 aagtaacttg aatggtctgc cgtgtatagg gaacagaaag cggagatatt gctaaggatc
     5761 agtctgccag ttctctcacc caaatgttgc ggaaactgac aggattgccg tgatcctgca
     5821 gcgttatcgg tcctttgtcg ccatgtgcct cataaggaac aacggagtgc ttgcttttcc
     5881 agagcgtgcc gccggagagt tccagatgat cctgaatcag gacaccattg tggagaatgg
     5941 taaatgttgc ccgcttgaca acgttgccgc tggcgtcaaa aacagggcgg tggaaaatga
     6001 tatcgtagga ttgccattcg cccggctttc ggcaggcatt tacaagcgga gcgcttcggc
     6061 cgtagagagc gcctgcctgt ccgtcggggt aagtcgtgtt gtcataagaa tccagaattt
     6121 gaatttcata ttgacccatg aggaatacgc cgctgtttcc gcgtccctgt ccgtcaccct
     6181 caacattagc gggggtggcc cactcaacat ggagctggca ggaaccaaat tcctgtacgg
     6241 tacggatgac accggaatct tccacaggct gaagggcacc gtctttcacg atccattggc
     6301 tcgggctgcc atcatcggca gaccagccgt cagtgttttt cccattaaac agaacaatag
     6361 catcagaggg aggcatgccg gtcacagcac cgggttgcac gacagggggc tgcggtgcca
     6421 gtgcggggtc tgtttcatgt acgagcacac cgtccaatga catgtactcc acttcttcca
     6481 cttcaccatt gtcgtttgtt tctttgacaa cccattcttt aatgactgga gtttcaaact
     6541 gtgcggacgc aaagggggta ccggtgagca ggagaagaaa caggataaag gggagaaata
     6601 aacgtgtttt catgaatttg agctccatcg tttaggttgg ggagattgga tccataccgt
     6661 tttggggaaa aagcagacca ttttgtactt ccggacagat ccattttaaa tgggtcagaa
     6721 tacctgagag ttattgtaca gatttttaaa tagaaaaaaa agaaaaggat aattaaaata
     6781 atttgcatcc ggatggctta aaataagaag attagagaca ggctgagaaa agcgtttttg
     6841 tcgtgcgtct acaacatcgt gttttaagta atctgtatgg atctcatgtc aagttaaagg
     6901 agtttgatca tgtcagtgaa agccattgca gaaagagcta tggagcaggg gcaaggcgtg
     6961 ctgcgcctta ccccgacctg ggtgcctcgt gttttttgtg tgcctggacg gcggattaaa
     7021 ctgcatcccc atgactacta tgctctggga ggggaaaggg gcggtattga cgaacgctgg
     7081 ttttcttcca caacgcctgc tgataacggc ccgctcacca gcaagaatga aggattgagt
     7141 cacatcgctt ttgaagatgg cggcaaaaca gaactcttcc tgcttcgtga tgccattgaa
     7201 gaattgaaag acgctattat cggaaaactc tgggctaaat atcaggcgtg gcccatgtac
     7261 tccaagtttt ttgataatca gggagccctt cctttccaca ttnnnnnnnn nnacattcat
     7321 catgatgatg atcatgctgc ccgggttggc gcacgtggaa agcctgaggc gtattacttt
     7381 ccgccgcagc tgaataatca cggaggcgat tttccctata cctttttcgg gcttgctccc
     7441 ggtgtgacaa aagaggaagt gcttgatgca ctgcgtgctt ttacaaaagg agaaaatcgc
     7501 attaccgatc tttccgcagc ctatgtgctg acgccgggaa caggctggaa cgtgccgccc
     7561 ggagtgctgc atgcgccggg cagtctttgc acctacgagc ctcagaaagc atcagatgtt
     7621 tttgcaatgt atgagtctgt cgtacatgat caggtgctgt ccgaagacct tctgtggaaa
     7681 gattcgccgg aagaggaaat cggaaaccac gagtatctgc tggaagtgat tgactgggag
     7741 cagaatacag atccgctttt caggcaaaag cattttatga aacctgtgcc tgttgatgac
     7801 cccgcggaaa tgcgtgctgc aggctatcag gaaaactgga tttgctattt atcaaaagaa
     7861 ttcagtgcca aagagctgac cgtgctgccg ggcgcaacgg tcaccatcaa agatggcggt
     7921 gcttatggcc tgtatgttgt tcagggacat ggcacgcttg gtatttggga cattgaatcg
     7981 ccggcgctta tccgctacgg tcagctgacc tatgacgaat attttgtttc tgaagcggcc
     8041 gcacgcgaag gtgtaaccat aaccaatccg agcaaatcag atcccattgt catgttgaaa
     8101 catttcggtc cggatgcgca taccaatatg cctgtcccgc tcgactgata taaaagagga
     8161 ggatcaaaca atgacaaatc agagcaataa tttccccaag ttacataacg cagcatggcc
     8221 cggtttggta gggaaaggcc cgggcagcga gcctcccatt gatctggata ccatgctgaa
     8281 tctgacagct gccgccgaag tggatggcat caagtttgac gggattgatc tttttcttgc
     8341 agatccgcat atcagcatcg atattgatga tgacggcatt aaggcgctgg cagacaagat
     8401 tcatggtctt ggtcttcagg tgggaagtct ggtcgcgcct gtctgggtgg atgcgggcgg
     8461 cggttccgcc atggatgagg aaaaagggcg gccccgcttt cttgagcagg tgcgcaaagc
     8521 gtgccgcatc ggtgcaaagc tgagagagct gggtattcgg ccggatggtg cggtgcgcat
     8581 tgattcggct gtctctgtag aaagctgggc gcaggatccg gaaggtaatc agaaaaaaat
     8641 tgccagaact ttcagtaagg ccgctgatat cgccgctgat catggagaaa ttctggtggc
     8701 cgaaggtgaa gtatgctggg gcggtatgca cagctggcgg cgcatgtcac agctccttga
     8761 aatggtggac agaaaaggac agtttggatt tcaggctgat atggcgcata cgctgctgta
     8821 tctgttaggt gccaatgcgc ccgaagatgc tctgcttcct cctgattttc aatggaatga
     8881 tgaatccgct tttgatcttg cctacaagtc tttgtgtgcc gccttgcgcc cctggacgct
     8941 ggattttcat gtggcgcaga atgatgcaac cgtcttcggt tccggaacgc atgataagac
     9001 tgggcggcat tgcctgcccg aagatccgaa tgggaaactt gatattgtta aatatgccgg
     9061 attctggctc cgtgacaatc agggaaatct gaagaaaaaa attcggcatc tgtgctggga
     9121 cggctgcatg tttcccaatg agactctgct gaatcctgca acctggaaca gcatcctcag
     9181 cgcaatgatt gctgtccgta atgcacatgg ctggcaggaa tgaacttaac agaaacacat
     9241 ccgctcataa aagaggaaag gtacaggata tgacacagat tcgtctgggc atgattggct
     9301 acggctttat gggacgcgct catacaaacg cattcaagag ggttcccgat tttttccccg
     9361 aactgaaata taaaccggtg cttcaggcag cctgcgggcg gaatcaggat aaggttaatg
     9421 cctttgccgc ccagtggggg tttgcatcga cggaaacgga ctggcggaag ctgcttgaac
     9481 gggatgacat tgacgcagtg gatatctgcg ctcccaatga ccgccacaaa gaaatagcac
     9541 tggcagctat tgcgcaggga aaaatggtcc tttgtgaaaa gccccttgcc atgaataccg
     9601 ccgacgggat cgagatggcg gaagcagcgg aaaaagccgg tgttgccaat acagtgtggt
     9661 acaactatcg ccggattcct gctgtcatgc ttgcaaagca cattgttgaa tccggaaaat
     9721 tgggacgtat ctatcactat agagcaaatt tccttcagga ttggtgcatc tctgaagacg
     9781 tgcctcaggg cggtgcttcc aactggcgcc tcaacattgc cgaggccggt tcaggagtga
     9841 caggagatct gcttgcccac tgcatcgaca cagcgctctg gttgaatggc tccattgata
     9901 aagtaacggc gatgacagag acctttgtaa aacaaaggat tcatgtcgaa acggggcagc
     9961 ttgcgcctgt cggtattgac gatgcctgtg cttttctctg tcgtttttcc aatggctctc
    10021 tggggctttt tgaatcaagc cgctatgccc gtggccataa agcgctctac acctttgaaa
    10081 tcaacggtgc caatgcttca atccgctggg atctgcatga tcttcatcgc ctcgaatact
    10141 tcgatcatgg tgatgaaagc gctctgcgcg gatggcgttc tattcatgtg agtgaccacg
    10201 gcggcgatca tccgtatatg ggtcgctggt gggtgcccgg tctgcagatc ggctatgaac
    10261 attcctttgt ccatcaggca gcggatttca tggagtcact ggaaaagggt cagccttgcc
    10321 cgcccacttt cagggatgct gttgaaacac aaaaggtctg tgaggcagtg ctgaacagtg
    10381 cagcttcaga aatctgggag aagatttaac ctcagggttc tgcttccttg ttgttgactt
    10441 ctgcgtcaat agatgagaca acttttttaa caactttgcg taaaggtttt ttctatgcgg
    10501 tactgtatgt tcgttatgtg ttgtgctttt tcgtgtgcct tttctctttc tgcggcatat
    10561 gggcaggaag acgctccgga cctggctttt gacagtgcgg tcgctgctat cggccaggat
    10621 gcgacagcga aaggcgcatt ggaagaggag tgtctgtcca cactgaaatc gtcagatgaa
    10681 tgggctgact tatatcgtgc cgttcgaatt ttatctgtta tcggcacaga gaagagcgtc
    10741 ccggtgttgt ccgagatgct tgttaagctt gaacagtctc acctcgcccg aattgcgctg
    10801 gaactgatcc cgggagaaga ggcctctgcg gccttgcgcg atgcactaac gaaagcgccg
    10861 gaaaaggaag cgatcgggat cgcttcttct ttgggcgtga gaaaagactc aggtgctgtc
    10921 tccgtattgg ctgatctggc agcaggagat caggaaggac ttgctcaggc tgcccttgca
    10981 gctctgggac acatttccgg taaaaaagca actgaagtct tgaaatccca tattctcgat
    11041 caaaacagag agcgtgccgc tgttgccgcc gaagcgctcc tcgtcgctct ggaagaggct
    11101 gagtctgata cggagacgac agccggcgtt tatgaatcgc ttctggaaaa atcctggccc
    11161 gacagaatcc gtgccggtgc ttttatgcgg atactggaag aggataccga aaaggcagtc
    11221 agtcatattc tggaaatggt ttattccaat gacttgtttt tgcgttctgt tgcgcttgct
    11281 tccgcaatca gcctgaagag cggtgatctg gtggggcagc tcgtagaaga actgccatct
    11341 cttgcggagg atgtacaggc gcgtttgatt gagtctttag gaaaacggga agaaggaatt
    11401 gccgaaaaaa cgctccatca gtttttgcac agtcccagcg aagcggtgcg tatagaagcg
    11461 gcaaaagcgc ttggaaacaa cggcaccagt gcgtcgattg ctccgttggc agaaatgatt
    11521 gcccgtgcag agggacgtac agaaaaaaat gttgccgccg agaccctgcg ccggctgaaa
    11581 ggaaaagatg tggatgcggc gcttgtggat tatctttcca atgcgccgct ggcagcacgc
    11641 cccgagatta ttgaagttct ggcccagcgc gatgttaaag aagctgtcat cacattgatt
    11701 gcacataccc gtatagatgg ctgcgcccct gcggcatttc aggcgcttgc gaaacttgcc
    11761 gatgtggatc aggtgcctgt gctgctgggc ctgctgactg ttcttgatgg cgacaaaggg
    11821 cgccctgagg cggaaaacgc agcagttgct ctctgcagaa aagcacagga aaaagatgcc
    11881 ggtattgaaa tccgcagtgc tgtctatggt gactggcctg atggtgcctg tgcggatgta
    11941 accgacaagg tgaagcaggc agtgcaggac ggcacttttt ccatcatcgc acagaatgcc
    12001 gattatggag atccggcacc gaacattgtg aaaaaactgc atctggaata tttagagcgc
    12061 ggagtcgtga agcaaaaaac tgtgtctgaa ggggcgacaa tgaatcttgc ttcagacatg
    12121 ctgccgctgc aagtctttga aatgatcaaa ggaaatctgc tggctaccac ggatgccaat
    12181 gcccaggtgt cttttctgaa ggttctcagt cgcctgggag atcccacaac cttcagtctg
    12241 gtgcaggaat atttggagca cagcgatcca cgcgttgttg acggcgctgt acgggctgtg
    12301 gctgcctggc ctacggtgca ggcgctggat acgctcgttg atcttaccca gggtagcggg
    12361 gaagcagctc agcagagcat tgctttccgc ggcgctgttc gtttgctccg tttgggagag
    12421 atttctcagg aaaagacact cgagtgctat aaatccctga taacggctgc gaaaaataca
    12481 gcagagcgca aattactgct ttccggactc ggagaaacag ggaatataga agctatttct
    12541 ctcattaccg ctctggcgaa gacagaagat cttcaggcgg aagccgatgc cgcgctgggt
    12601 cgtattaaag agaaagtggg tgaggaaact tttaataaag caatgaaccc agggggggcg
    12661 gctccgctta taacaggaaa atttgtttct atctttgacg gaaagacact ggacggctgg
    12721 tcgggaaatc cccagtactg cagggttgaa gacggctgta tcgtgctgga aacaaagaat
    12781 gcggatgaac tcgagcacaa tactttcctc agctggaacg tcgaggaact cgaagatttt
    12841 gagattatgt tccaataccg tattgacagc gagtgggcaa attcaggcat tcagatccgc
    12901 tccgaatatt tctcggatga acagttcaga gtgcgcggat atcaggctga tatctccaat
    12961 gaagactggg tcaccggact gtgctatgag gaaggcggtc gtcagtttct ggcatatcgt
    13021 ggtgaaaagt cagtggtcac agatgatgga gttcaatcag aacgctttgc cgatcagaaa
    13081 gcgctcggcg actatatcta tcctcacgac tggaaccaat atcatgtgat cgtaaaaggg
    13141 gacacttttg tttccagaat caacgggcac acgatgcatg aagtgaccga ccattcctcc
    13201 gttgcgaaac ggcgtggtat tatcgccttt caaattcaca aagggcctcc catgaaagtc
    13261 ctttttaaag atgtcaaagt taaaaaactt gaacctgcct ccaactgaaa acacaatagc
    13321 gtcaatgctt accataaagg atactcaata atgaagtcag tgaaaacact tacacggcgc
    13381 gcttttctcc gccaatccac aatggcggct ggtgctcttt ccgtgtcccc tctggttatg
    13441 tcccgtgcct tcggtgatgc caatccttcc gacaaaatca ccgtaggtgc cataggtata
    13501 gccggacgag ggcgttacat tctgaatgcc tttcttcaat attctgatgt gaaggtcgtc
    13561 gctgtttgtg atgtctttgc agacaggcgc aacagagcca aacaaatggt cgatgaacat
    13621 tacggaaatg acgactgtgc tgcttatatt gatgcgcggg aactgattgc ccgacctgac
    13681 atagacgcgg tgatgatcgc cacaggtgac aataaccatg cccctctttc cattttggct
    13741 gcccgggcgg gcaaagatat ctactgcgaa aaaccgatga gcgtaaccat tgcggaaagc
    13801 cgggcggtat cggatgcggt acgccacaat ggacggattt ttcagtgcgg cacccaacgc
    13861 cgtaatgtcg gcaattttgt acttgccgta gaactggcac gttcgggtgc tctgggcgaa
    13921 atacaaactg tgtatgccga aaaagcctgg cctgattcca ctatttacta cggggttctt
    13981 ccggaagaac ccctgccgcc ttacgaagaa atggcttggg atttatggct cggcccggcg
    14041 gcgtggcggc cgtacaatct gaaatacacc acaggattct ggcggagaca tggtgatttt
    14101 tcgggaagtt ccatcgccga atggggctcc catacggtgg atctgtgcca gtgggcttta
    14161 aatgccgatg acacatctcc tgtgcattac aggatcatga atgaactggg cgacatagaa
    14221 gcacgctatg cgaatggtgc aaaacttgtt attaccaaag gcttgcgttt tggatcctgc
    14281 cccgttcggt ttgaaggcac tgaaggctgg gtggaaacgg gagacgacaa caccattgaa
    14341 acatctcccg cttccctggc aagcgatcgt catttttccg gcggctatcc ggcagataat
    14401 catgtgcgtg aatttctcaa tagcgtcaaa agcagacagc cgaccaagtc aacggctgaa
    14461 acgtcacatc gatccattac cgcctgccat tgtgcaaaca tcgcaatcaa attaaagcgc
    14521 gaagtgacct gggatcctgt gaaagaagcc tttgttaacg atgatgaagc gaacagactg
    14581 cgcagccggg cctaccggga accctggcgg atgtaagcag ttttcatctc tgaaataaaa
    14641 aagaagcagc cgtcaatatc atattggcgg ctgctgtgtt tttctgtggt acgcaattct
    14701 tagagcagga tagattctgt ctggcggtca aattttacca ccttactttc acgcaaggac
    14761 agataactca tgatcagcgc cgtctgcacc cgatacgcca gctccatatt actgatcgta
    14821 ttcggattgc tgttccggaa acagccgata aactcccgga aaaagtgcag caggttttcc
    14881 ccacggtcaa tgtcaaacag ctgtttttct ttattggagc catgagcggg tgtaaagata
    14941 attttgtcat tggagactgt taatgctcca tcccatccgc ggattacagg cactcgcgaa
    15001 gcggaaccgc ggttctcgct tgcctgaaaa tcatttccct gcgttcccag cacggcaata
    15061 gtgaagtttt caggaaaatc gacaagcata ttgaaagtat ccggcacttc acgctcgctg
    15121 tagcggaatt ttcctcctgt tgccaccgca acggacggca gaccggcatt catcatgtgc
    15181 atgaccggag ttagaatatg cgggaaaaga tccgtgcaag gacctcccgc atagtcttcg
    15241 tacatgcgcc agcggaagaa gcggcttaca tcaaaggcgc gctgcggcgc atcgccgaga
    15301 aaagcttccc agttcagatc agcacccggt cgggcattgg ggtcatcgat gggcatgccc
    15361 gcttcgcccc agtcgcctac ccgaaaatac ccgcattccg catggatggg ctgcccgatg
    15421 agtccttcgc ggacaagctg tccaatctgg cgccaggctg tgtcggacat gccctgggtg
    15481 ccgcacatga acacaccccg tgatgcggca acttctttgg caagctgttt tgtgagttca
    15541 aactgcctcc agtgtgtcac cggtttttcg caatagatgc ctttgccggc gtgcaatgca
    15601 tcaagtgcct gatacccatg aatatggtca ggagttgcaa tggtgacaat gtcaacggat
    15661 ggatcttcaa gcaattccag atggctcatg tagcctttaa tgccgaaacg ctccgctgtt
    15721 ctggtcatac ggggtctgta tacgtcacaa acagcacgaa gctcaatgct tttgtcttcg
    15781 tcctgtatat gtttgagggc atcaagatgg ctgttgcctc tgttaccggc accgataaca
    15841 cctacgccga gacgctcgtt agcgctgtaa cctctgcggt gaagcccggc actgagtccc
    15901 gctgctatgg ttccggtgac agcggcgtgg tgaaggaaag aacgtctggt taaagggggc
    15961 agtgcagtcg ggttttcaga tttcatatgc aattactcct gcaggtcgaa caagaacagc
    16021 aacggtaacg aaggattgat tgagagcaaa ccaaagattt tacaattttc cgtgaagttt
    16081 caacttcatt ttttctttga gagataaatt agcctctaaa aggttcgttt catgaacaac
    16141 aatgccttca agcagcgcat ttttaaagac tgccatgcga acgctgcact ggcttatcgt
    16201 ggcattttgc aggtttgtca tataaaaatt aaccagatca agtttagcgg aagataagac
    16261 cgctcccgaa aggtctgccg tcgaaaaatc acatttagtc aggtctgact caaaaaaact
    16321 ggcattctta aagttcatgc cgtgaaagtt gtactcgtgc aggacaacat tgtttaaggt
    16381 tgcgttttca aaagaggcat ctcgcaggtc agcgttgagt aaatcagcgt tacttaattc
    16441 ggcgctattt aaaatagctc cggctagttt ggcacctaga aaattggcac caaaaaaatt
    16501 acattgcgtc agatctgtac cccgtaggtc caccttggat agattggcat gagaaagatt
    16561 ggtgaatgca aggtcgagcc gatgcaaatc cagaccggac aagttcaatc cgctcaggtc
    16621 ggggcgggaa tgccgggttt cttttctcca ggcgttccag gcgtcaaccc ctttctgaag
    16681 gcattccaca tgctgcctgt aagccataac atccgctcct gttcaccgca acgaaataga
    16741 tacatggaaa acataaggac gctgacaagt atcattgtac gtacttgaac gggagagttc
    16801 aaagttgtgt gccagaagtt gtgtgccaga actttacaca tcgaaaccaa gagcggagtc
    16861 attcttttgg acggcctgca tcactggaga gcgggaaagt gaacgacttg cttttgtgat
    16921 tgggcgtctg aaaaatcgtc agtcggattt gtagttgttc gaaagcaccg gtctttttaa
    16981 ttctgtccgg atcaagcctc aaagaaacct cttcctgaga aaatatttcg tagtctgccc
    17041 ccgcatttat ccaaaaagat tttatctggc ctgactcgca gcgttgctat agaatcggct
    17101 ttagcgtatt ataggaaagc ggtcaagaaa gcagcgtgga gcatactatg ctatcctttt
    17161 tcacaaaaaa gaaagagatt ccttcccctc ctctacctcc cggcgaggtt ttccaggatc
    17221 cttttgctga agagatctgt caagccccca ttctttggtc cagtcgttat gagagtttcg
    17281 ttgtttcatg at
//
LOCUS       DAOPWB010000068        17236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3373_length_17236_cov_4.603341, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000068 DAOPWB010000000
VERSION     DAOPWB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17236
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3373_length_17236_cov_4.603341"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PKN92_07715"
     CDS             complement(<1..293)
                     /locus_tag="PKN92_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48717.1"
                     /translation="MSDMWELASYVEEHPGDYPQRWQLAKKLYAAHEYRLALEHLLVL
                     KNEWKPHLNSRRYLGAVYYRMGRYTEAAKTLREAIREWPEEIGLREQLAHALTR"
     gene            complement(338..625)
                     /locus_tag="PKN92_07720"
     CDS             complement(338..625)
                     /locus_tag="PKN92_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48718.1"
                     /translation="MKVLKILKKPAVLGWLLWFITTLLLAGPAVMLMYRITYDTANAL
                     TRIVSGVFGAAIFSGVLVTLGNEIWFRIRRKQLAQAKKENRRAKKKSGKKK"
     gene            complement(603..1943)
                     /locus_tag="PKN92_07725"
     CDS             complement(603..1943)
                     /locus_tag="PKN92_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ48719.1"
                     /translation="MNNSRQKPTYDHRGFPACLEDYQPWLLEATQEELRRVVATMHRV
                     HGFTAVLTDLDLLLERISEEGRLVAQAEAASVMLYDKKADDLYFRVVLGDKSSPDVLK
                     KEVRLKMGQGIAGAAAESRQVIHVPDVSKDPRFYSGADAASRFQTHSLLAVPMIERGE
                     LIGVLEVVNKIGDICFSAMDQHVLEVFSLLAATAVVNARLIEAQIQNERLAAIGQAIA
                     GLTHHIKNIMTGLGNCVELIDGAIKKEDFLLVRQIWPILFRSTGRISRFVQDLLLAAK
                     PRMPVMAPCWLHQIVADVCEDVREQCAHKSVCLEVQAKENVDPIVGDADLLSRCILNL
                     LINAVEAAPLKNGRVTITLGLKNDGARTIEVCDNGPGIPQQARARIFDLFYSEKGTRG
                     TGLGLASSYKIAQEHGGTLELLDSTGGACFLLTLPQRREMANIFGGSNESSENP"
     gene            2366..3490
                     /gene="proB"
                     /locus_tag="PKN92_07730"
     CDS             2366..3490
                     /gene="proB"
                     /locus_tag="PKN92_07730"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNZ48720.1"
                     /translation="MPSKASKRIVVKIGTNLLTGRRAFDGHILEGLVQEIVSLKQDQG
                     MDVLIVTSGAVGCGMDALGLVKRPTALPEKQAVAAVGQARLMHYYETLFRVYGKGMTT
                     AQILLTQADLDSRQNYLNIRNTLSTLFTMKSVVPVVNENDSTATEELRFGDNDTLAAK
                     IAAKANADLLIILTDVDGLFDKDPREDPTASLIREVTQITDEIEALAGGAGSIASTGG
                     MRTKLQAAKIAVAAGVDCIITSGYRKRVLHQILAGEAPHTNFRPAKVALSHRKRWIAF
                     GRSICGTLHVDAGATAALVEKGKSLLPVGVQKVEGRFGAGAAVRIVDDAGAMIACGLA
                     NYDSETIDLLKGKKTEEITSILGHNDYDEIVHRDNLVLMH"
     gene            complement(3507..3722)
                     /locus_tag="PKN92_07735"
     CDS             complement(3507..3722)
                     /locus_tag="PKN92_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48721.1"
                     /translation="MAKIVWRYRLTNQEQQLWEREELRGWRAAMTGFVEDEARDRGCL
                     KFAIYSTDEVLILKDSVTRDHEESAED"
     gene            complement(4124..5821)
                     /locus_tag="PKN92_07740"
     CDS             complement(4124..5821)
                     /locus_tag="PKN92_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48722.1"
                     /translation="MSERPITDPAGRSGGESETFALEQELLALGGELSSLMPAIHLET
                     AVMAGLASFEDAEAGLEEELLLLGDALAGAVPEAGLQQRILERIEAQVCQENTELKTE
                     LRAIGAEMREKQPVVDIAEPVLDAVPARTLSETVDFTRHRKQRRRESWASRQMLLTAA
                     ACLLLVFGVLFTRVIRPELTRLPGSVLQTARTQASGSEESASAKKEAREGEALSLTMG
                     SSQGEIVLSALKRPAPYTEEQGTEQKARHEEHSLQDILDLRRQAADGKSDALALLARW
                     GALDPDQARKLLAEGSLSPADLVALSRFLPADEAMALLREAIEVNPEDPGLRLALARM
                     LLADPKNHAEAQRMQEALRNLDPGNSLAYFMDAQVRFAAGDYVNALESLQEASRLSFA
                     SAYGLSNAQHHRAVLEAAGYPAETAGALAAFYAGTEEYGYLEQMRNDLLDYGSYFESI
                     GDYESAFAIYKSIGELGGQLLNGAAFGNEQLAGLDMQTAAMEAISQLLQILKVPGASQ
                     VVESSYELFAEGLDLFLENLGRLDDYLKQGSGNSVVETINGIMQYGDIRFFPRERKE"
     gene            complement(5818..6408)
                     /locus_tag="PKN92_07745"
     CDS             complement(5818..6408)
                     /locus_tag="PKN92_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ48723.1"
                     /translation="MEVSTECSDEQLMALLCEGNTNALALLVERYQNDIFRFCIHYLR
                     DLEAARDAAQDIFLKVFAAKDKFDTSRSFRPWLLRIARNLCFNLLKRAQIVPMESLDA
                     MQEKEGGSRQYWKAQTSAHPLEGILASERRRVLLRIVDTMPEDMREVIVLKYFEQMSS
                     KEIADVLEITEGAARTRIHRAIAWLRTKNKVITDLQ"
     gene            complement(6703..7359)
                     /locus_tag="PKN92_07750"
     CDS             complement(6703..7359)
                     /locus_tag="PKN92_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNZ48724.1"
                     /translation="MEAMEKIVTCVTRGKVDQNSPWPPDLKGEAGVFELVRDALAAGV
                     DPNEILAKGLMPGMEIIGKKFSQGTSFVPDLLMAAKAMNAGLEQLKPFFEEGTAARRG
                     VFVLGTVQGDLHDIGKNLVRMMMEGAGYEVIDLGANVAPETFLENVKKFPGCIVGMSA
                     LLTSTMVNMETTARLIKKESPDTLVAVGGAPLSEEFCTAIGADLYAADPQQLIERLHA
                     "
     gene            complement(7848..9146)
                     /locus_tag="PKN92_07755"
     CDS             complement(7848..9146)
                     /locus_tag="PKN92_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNZ48725.1"
                     /translation="MASCNQIEDMLQTYLDGELGRVERLLLEEHVEVCPSCRQELQRA
                     RACAAHLYETLHRDRRTVDLVTGVMAHLPEMKSARRTWRDTSSARSASNYGKQPLRNP
                     VFRYMPVYVPALLFILALLLWMQWPERINEQADSVGVVTYTSGTAYLANAVSSRSSHL
                     KAGALLAAGDTLDTAENGQMLLALKGPTHLAVYGNSSVCITSDRQILMDRGRMFLDVF
                     RDSRRFTITTPHGCITVMGTSFQVGIEDSRTVVSVVNGDVLVENDHSFARLTRGKQAV
                     FQKDSGMRIASLADVDRFVEEARALTPDIQAERFFRASILPAPPESSPLTGEQVFVVK
                     TRSRNVKALLLRWVPDPYLAGHAAYTVYISDNAMRPVSKYTIIPHVFQEKHRNTFEIR
                     MPEGLQNRDLSVLHVTVLPVQNSGHVETSFTEVLAVGAKE"
     gene            complement(9125..9721)
                     /locus_tag="PKN92_07760"
     CDS             complement(9125..9721)
                     /locus_tag="PKN92_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNZ48726.1"
                     /translation="MHRTEGELVRKSLQGDTDAYAALVQRYQDAVYATAFYYAGRYGG
                     AEDIAQEAFWKAYRNLHQLKDQEHFGAWLKVITTRTAADWLRRFAPRHEGYATPLPHR
                     RAREAQAEEAAAPGVEADDYALVHRAIQKLPERYQLPVVLRYVQEMSYKEICRFTGSS
                     YDEIRGILNRAGKLLRQELTAQQKQAEEGIIPWRRATK"
     gene            10034..>10278
                     /locus_tag="PKN92_07765"
     CDS             10034..>10278
                     /locus_tag="PKN92_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ48727.1"
                     /translation="MVSDRLLIFDIDGTLLQTDTVTVPAVQQAFRDYGLPEPDKEAIC
                     FFFGKPVEDYEAWMASLCAEGKAEEVVSHANALELQC"
     assembly_gap    10279..10288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10289..10698
                     /locus_tag="PKN92_07770"
     CDS             <10289..10698
                     /locus_tag="PKN92_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNZ48728.1"
                     /translation="CMHTCGLLYPKALETVQALHDRGYQMAICSNGPEPYVAAFVEAH
                     GMAPFFSTVYARGTRYRDKTEMTGLILEQHRPAAFAVIGDRHDDIEAALAHGGIGIAA
                     AYGFGSAPEYEQAERVLASIAELPECLNALWHC"
     gene            complement(10700..10966)
                     /locus_tag="PKN92_07775"
     CDS             complement(10700..10966)
                     /locus_tag="PKN92_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48729.1"
                     /translation="MVNPELLKILVCPENKTPLHPAAPEVLDALNAEVVAGTLKNRAG
                     DTVTDLIQEGLIREDGAVLYIVRDDIPVMLIEEAVPLAKDGSDC"
     gene            complement(11005..12165)
                     /locus_tag="PKN92_07780"
     CDS             complement(11005..12165)
                     /locus_tag="PKN92_07780"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNZ48730.1"
                     /translation="MNLEAIRAKKSRFAIGLMSGTSCDGVNAVLVRIKGTGPGLAIKL
                     LAHQTFPYTTDFQNKLLVEHMSAKEVCLLNFELGQLLAEASLAMLDQADDHEVEVDFI
                     AAHGHTVGHYPPGSGNDRVGTLQIGEPAIIAHQTKLPVISDFRVRDMAAGGQGAPLVP
                     YADWLLFQREEKNIVCLNIGGIANFTVVTPKFEDILAFDTGPGNIAIDATVRLLSRGS
                     RAFDENGDAARRGKVIDEFLEYLLSDSYFQKEPPKSTGRERFGLEAYLRDALASRRNH
                     SYEDLVATVTEATVRSIADAYRNYIAPNYEATQMIVGGGGAMNNTIMTRLQQELPEMK
                     IFISDRVGISYDAREAIAFAILGNETIFGTPANVPQATGASESVVLGKITPP"
     gene            complement(12165..13778)
                     /locus_tag="PKN92_07785"
     CDS             complement(12165..13778)
                     /locus_tag="PKN92_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNZ48731.1"
                     /translation="MSSPLCIVSNKPYKKRSVSVLRAVFAFFALSCLAMLCSSAEEAV
                     NPLEVKARIRYAEMKEKFTFRLQEKGTLESGDTLYSLAPGAYTLTLEKGRGATQHYHV
                     FTRTFAPAEVQEKEACLREWQEKKFTPQVITFGLLYRLPSGRTLDNRRHWVSVARCKE
                     KSEAKALADRLKTGSVAPWIRPERIAPGTASFSLQGPRGKASSLFAAPLFLDCEGVVE
                     IVDVSGTFWKEKKRNCLYAPPLEIGIGAEDTIEIFGTLPVEEYLRGVVPAEMPVQWPL
                     EALKAQALVARSEIYASLAGKYKLQGFDFTTLESCRAYWGAGEFHPSSDRAIRETAGE
                     ALSFDNRFAVAVFSSCCGGWTENNDTVWSGPPDRLLRGGSDLRENGGSVTPAKNMLQW
                     LQSAPRAWCEGDQQSFRWQRRFSTDELSALINKKYKVGTVLSVTEGVRGVSGRLKSVT
                     IVGTLSTVTIERELAIRQAFGGLPSAMIIIKADPPGKTPAHWKVYGGGRGHGVGLCQH
                     GARGMAAAGLDYRDIVAHYFSGAVIERTR"
     gene            14063..15652
                     /gene="groL"
                     /locus_tag="PKN92_07790"
     CDS             14063..15652
                     /gene="groL"
                     /locus_tag="PKN92_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960418.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNZ48732.1"
                     /translation="MAKQLLFDQEARIALLSGVDQLADAVKATLGPKGKNVLLEKSFG
                     APKITKDGVTVAKEVELEEPYANMGARMLRQAASKTNDVAGDGTTTATVLAQALVREG
                     MRHVTAGANPAHLKRGLDKAAEVALDAIKAAAKSIKNQDEIRQVAIVSANGDTSIGDI
                     IAEAIAKVGQDGVITVEEGKGFATEMEQVDGMQFDRGYLSPYFVTNRETMQVVLDDPY
                     ILCYEKKISNISEMLPVLQKIAQAGKGLLIIAEDIEGEALATLVVNRLRGILNVAAVK
                     APAFGDRRKEILRDLAILTGGQFISEDLGMKLENVELNQLGTAKRIEITKDHTTVIQG
                     GGDKKEIAGRVDQIRKGIETTTSDYDREKLQERLAKLAGGVAVIQIGAASEVELKEKK
                     DRADDALNATRAAIEEGIVAGGGVALIRARKSVLDLDLQGDEKTGALMLAKAMTEPLA
                     IIAQNAGFEGAVKVNEVSVAKGNIGFNAATGVMEDLVKAGIIDPAKVVRTALQNAVSA
                     AGMIISTECLITDAPKKDDEK"
     gene            16002..16886
                     /locus_tag="PKN92_07795"
     CDS             16002..16886
                     /locus_tag="PKN92_07795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ48733.1"
                     /translation="MDQFEEGQKIASRYIILRKLGRGGMGEVYLVEDTTCGEQRALKT
                     LRSKYLKSNHAKARFVREATTARQLTHPGIVTIYDTGNWEDTLFYTMDYVDGKSLRQW
                     MHQWGRFDIGSTVRILCLVADALVHAHRLTVHRDLSPENIMVLADGSIRILDFGLAKS
                     DDKFKNLTMVGVNLGKIAYIAPEQHMDASRVDKRADIYPLGIMFFEMLTGRRPALGDK
                     ISRICPELPRETDVFLAKAMARNPNDRFPSAQVFQSELLALYQRVRDWEIKRNTFFMR
                     IWIAVTNLFQRKQARPPL"
BASE COUNT         4281 a         4728 c         4301 g         3916 t
ORIGIN      
        1 cgggtcaatg catgcgccag ctgctcacgg agtccgattt cctccggcca ttcccggatc
       61 gcttcacgca gcgtttttgc cgcctcggta tagcggccca ttcgataata tacggcaccg
      121 agatagcggc ggctgttcag atgaggcttc cactcatttt taagcaccag caggtgctcc
      181 agcgccagac gatactcgtg ggcggcatag agtttttttg ccagctgcca gcgctgagga
      241 tagtctcccg gatgttcttc cacataagag gcaagttccc acatgtcaga cataagacag
      301 tccttatcaa ggaaaggata ctaaaagggg cacgcagtta ttttttcttc ccggatttct
      361 tttttgcacg acggttctct ttcttagcct gagcaagttg cttgcgtcgt atccggaacc
      421 agatctcatt gcccagtgtc accagaacac cggaaaagat ggcggcacca aaaacacccg
      481 aaacaatgcg ggtcagtgcg ttggcagtat cgtaagtaat acgatacatg agcatgaccg
      541 ccggccctgc caggaggagc gtagtgataa accaaagaag ccatccgagg acggctggct
      601 ttttaaggat tttcagaact ttcattggaa cctccaaaaa tatttgccat ctcccgccgc
      661 tgcggaaggg tcaaaagaaa acaggcgcca ccggtactgt ccagcagttc cagtgtgccg
      721 ccatgttcct gtgcaatttt gtaggaagaa gcaagcccga gccccgtgcc ccgggttcct
      781 ttttcagaat aaaaaagatc gaaaatacgg gcgcgcgcct gctggggaat tcccggaccg
      841 ttgtcgcaaa cttcaatggt gcgtgctcca tcgtttttca ggccaagcgt aatggtgaca
      901 cgtccgtttt tcagcggggc cgcctccaca gcattgatca gcagattcag aatacagcgg
      961 gaaagaagat ctgcatcccc cacaatggga tccacatttt ctttcgcctg cacttccagg
     1021 cacacacttt tgtgcgcaca ctgttcccgt acatcctcac acacatccgc cacgatctgg
     1081 tgaagccagc agggtgccat cacaggcatg cgaggctttg cagcgagaag aagatcctga
     1141 acaaagcgtg agatacggcc tgtgctccga aaaagaatag gccatatctg gcgaaccaga
     1201 agaaaatcct cttttttaat ggcaccgtca atcagttcaa cacagttgcc cagcccggtc
     1261 ataatatttt ttatgtgatg ggtcaacccg gcaatggcct gccctattgc ggcaagacgt
     1321 tcgttttgaa tctgcgcctc aatcaggcgc gcattcacaa cagccgtcgc tgcaagcaga
     1381 gaaaatactt ccagcacatg ctgatccatg gcagagaagc agatgtcccc tatcttgttc
     1441 accacttcca ggactccgat taattccccg cgttcaatca tgggcacggc gagaagactg
     1501 tgcgtctgaa agcggctagc tgcatctgcc ccgctgtaaa aacggggatc tttgctcaca
     1561 tcgggaacgt gaatcacctg cctgctttcc gctgcagcgc cagcgatccc ctgccccatt
     1621 ttcaagcgca cttctttttt cagtacgtca ggactgcttt tatcgcccag caccacacga
     1681 aaatacaaat catccgcctt tttatcgtaa agcatgacgg aagccgcttc cgcctgtgca
     1741 accagacgcc cttcctcact gatacgctcc agaagcagat ctagatctgt cagtactgcc
     1801 gtaaaaccgt ggacacggtg cattgtggca acaacccggc gcagctcctc ctgggtcgct
     1861 tcgagcagcc agggctggta atcttccaga caggcaggga atccgcgatg atcgtatgtt
     1921 ggtttctggc gactgttatt cacggaaaaa gaatcttcct ttgcaggatt ccactggaac
     1981 ggtggaaata ctggcggtga ctgttgaaaa cagcctggtt cggaccgaaa cgaggcataa
     2041 ccagtgtacc ccacaaagag cgctgatgca aatattatcc tgaaaaaatg ccgcttcccc
     2101 agcaccaccc ggcagattaa acagatacct cttccaagcc tttcgcccgt ttcagtgaag
     2161 gcgggaatca gatcgtctcc ggtgtgagcg ccggcgcatt ttcctctgag gaaaagaaac
     2221 cgggcgcacc aaataagctc tgttcaaaga atgatccgtt aaaattcttc ggcggacatc
     2281 tgttttggaa gcgcaggaaa aaaaccgtat aataagactg tggcaatgca aggtaaatac
     2341 aggcagaatt ggagtcccta tgtctatgcc ttcgaaagct tccaaacgga tcgtggtcaa
     2401 aataggaacc aatctattaa ccggcaggcg cgcctttgac ggtcatattc tggaaggact
     2461 ggttcaggaa atcgtatctc tgaagcagga tcagggcatg gatgttctta ttgtcacatc
     2521 cggtgcagtc ggatgcggca tggacgctct gggtctcgtt aaaagaccga cggccctgcc
     2581 tgaaaaacag gcggtcgcag ctgtcggaca ggcccgcctc atgcattact acgagacgct
     2641 cttccgcgtg tacggcaaag ggatgactac agcacagatt ctgctgactc aggccgatct
     2701 ggactcccgg cagaactacc tgaatatacg caacacactc tccacccttt tcacaatgaa
     2761 gtcggtggtg cctgttgtca atgaaaatga ctcaacggca acagaggaac tgcgtttcgg
     2821 agacaacgac acgctggccg caaaaattgc tgccaaggca aatgccgatc tcctcatcat
     2881 tctgaccgat gtggacgggc ttttcgataa agatccgcgt gaggacccga ccgcttcact
     2941 cattcgggag gtgacacaga tcacggatga aatcgaagcg ctggcgggcg gggcgggaag
     3001 tattgcttcc accggcggga tgcgcaccaa actgcaggcc gctaaaattg cagttgccgc
     3061 cggagttgac tgcatcatca cctcaggata cagaaaaaga gtcctccacc agatcctcgc
     3121 gggcgaagcg cctcatacga atttccgccc ggcaaaagtg gcgttatcgc acagaaaacg
     3181 ctggattgcc ttcggaagaa gcatctgcgg cacgttgcat gtggatgccg gtgccaccgc
     3241 tgctctcgtg gaaaaaggca aaagcctgct gcctgttggc gttcagaaag tggaaggacg
     3301 cttcggtgcc ggggcggcgg tgcggatcgt tgatgacgca ggagccatga tcgcctgcgg
     3361 tcttgccaat tacgacagcg aaacgattga cctcctgaaa ggcaaaaaaa ctgaggagat
     3421 cacatctatc ctcgggcaca atgattatga cgaaattgtc cacagggata atttagtgct
     3481 gatgcactga tgctcagaag aacacttcag tcctctgcgc tttcctcatg atcccgggtc
     3541 acactgtctt tgagaatcag cacttcgtcc gtgctgtaaa tggcaaattt caggcagccg
     3601 cggtcacggg cttcatcttc aacaaaacct gtcatggcag cccgccagcc cctcagttct
     3661 tcacgctccc ataactgctg ttcctgatta gtcagtctgt aacgccaaac aatttttgcc
     3721 atttcagaga ctcctttcgt gaatggacga gccagaaagc gctcttccat gctggttatg
     3781 ccgtgccaga caacgaacac cgccgggagc cggaaacagt tgttttcagt cagcaaaacg
     3841 aggagcctgc ccttttacag ctgcccggaa agaaaacgcc tgaaactcgt cttcttttcg
     3901 cgtctggcgg tccgattact tcgggttcag ccctaaattg ttcaattagc ataacatata
     3961 gccgggctaa aaacaaatgc aagtggcata gggcatgttt tcccctcttc cgctgccttc
     4021 tcttttactt ttttcacaga taaaaggaag agagaatgaa gggacaggtt tcggaaggaa
     4081 aaagcggggg gcaggcaaaa agactttata aagggcggtg ccgttattct tttctttccc
     4141 gtgggaaaaa acgaatatca ccgtactgca tgatcccgtt gatggtttcc acgacagaat
     4201 taccgcttcc ctgtttcaag tagtcatcga gacggccgag attttcgaga aaaagatcca
     4261 gtccttctgc gaaaagctca taactgctct ccactacctg agaagcgccg ggcactttca
     4321 aaatctgaag caactggctt atggcttcca ttgccgctgt ctgcatatcc agcccggcaa
     4381 gttgttcgtt tccgaaggct gcaccgttta gcagctgacc gcccagctct ccaatgcttt
     4441 tataaatggc aaaagcagat tcgtaatcac ctatggactc aaaataactt ccatagtcaa
     4501 gcagatcatt gcgcatctgc tccaaatagc cgtattcctc agttccggca taaaatgcgg
     4561 caagagcccc tgccgtttct gcgggatatc cagcggcttc cagcacagcc cgatgatgct
     4621 gcgcattgga caatccgtaa gcgctggcaa aactcagacg ggaagcctcc tgcagtgatt
     4681 ccagtgcgtt tacataatca ccggcggcaa agcgcacctg tgcatccatg aaataagcca
     4741 ggctgttgcc gggatcaaga ttgcgcagcg cctcctgcat acgctgtgcc tccgcatgat
     4801 tcttcggatc ggcaagcagc attctcgcca gagcaagccg cagccccggg tcttcaggat
     4861 tgacttcgat cgcctcacgc agcagagcca tggcttcatc cgccggcaga aaacggctca
     4921 gtgccaccag atccgccggg ctaaggcttc cctccgccag aagtttccgc gcctgatcgg
     4981 gatccagcgc accccagcgg gcaagcagag cgagagcgtc cgattttccg tccgccgcct
     5041 gtcggcgtaa atccagaata tcctgcaggc tgtgctcctc atgccgcgcc ttctgttccg
     5101 taccctgttc ttccgtgtaa ggggcgggcc gctttaaggc ggacaacaca atctccccct
     5161 gactgcttcc cattgtcagg ctgagcgctt cgccttcccg ggcttctttt ttcgccgatg
     5221 cactttcttc cgaaccggaa gcctgtgtcc gggctgtttg caggactgat ccgggcaggc
     5281 gggtcagttc cgggcggatc acccgggtaa aaagcacacc gaaaaccaaa agaaggcagg
     5341 cggcggcggt caggagcatt tgccgcgatg cccagctttc acgacggcgc tgcttccggt
     5401 gccgtgtaaa atcgacagtt tcagaaaggg tgcgggcagg cacggcatcc agaacaggtt
     5461 cggcaatatc cacgacaggc tgcttttcgc gcatttctgc gccgatagcc ctgagctctg
     5521 ttttcagttc cgtattttcc tggcacacct gagcttctat ccgctccaga atccgctgct
     5581 gcagaccggc ttcaggaacg gccccggcaa gggcgtcgcc cagcaaaagg agttcctcct
     5641 ccagcccggc ttcagcgtct tcgaaggaag cgagccctgc cataaccgct gtttccaaat
     5701 ggatagcagg catgagcgaa gacaattcac cccccagcgc cagaagctcc tgctccagcg
     5761 caaaagtttc agattcgccg ccggatcttc cggcaggatc ggtaatgggt cgttcgctca
     5821 ttgtaaatcc gtaattactt tatttttagt tctaagccag gcaatcgccc gatgaatgcg
     5881 cgtccgcgcc gcaccttcgg tgatttcaag aacatcagcg atttccttgg acgacatctg
     5941 ctcaaaatat ttcaggacaa tcacttctct catatcttca ggcatcgtgt cgacaatgcg
     6001 cagcagcacg cgccgccgct cgctcgcaag tatgccttcg agcggatggg cggaggtctg
     6061 tgccttccaa tactgcctgc tgcccccctc tttctcctgc atggcgtcca gactttccat
     6121 aggcacgatc tgggcacgtt tcaacaggtt gaaacagaga tttcgggcga tacgcagcag
     6181 ccaggggcgg aaggaacggc ttgtatcaaa tttgtccttc gccgcaaaga cttttaaaaa
     6241 tatatcctgc gcggcatcac gggcggcttc cagatcacga agataatgaa tacaaaaacg
     6301 gaatatatcg ttttgatacc gttccaccag taaagccagc gcgttcgtat ttccttcaca
     6361 cagcaatgcc atcaactgtt cgtcgctgca ttcagtagag acttccataa cgttctccgg
     6421 gcgctgcccc gcagggtcag ccgctgatga gcctttctga attgcatcca tggattctac
     6481 aatctaactc attggtttgt tacggtttaa ttacctgaag cactgtacgg aatgttttat
     6541 gccagatact gtgggtgcat acctatacac atacaatatg tagaaacgca cgattttcca
     6601 tcatagaaaa agggaaaaca cctgcgcccc gacaatgatc cggtgcctgt gttttccctg
     6661 tcagaaaatc atactgcaat ctgatacgga tcatgctgta gtctatgcat gaagcctctc
     6721 aatcagctgc tgcggatcgg cggcgtaaag atcagcaccg atggctgtgc agaactcttc
     6781 tgataaaggc gcgccgccca ccgctaccag cgtgtccgga ctctcttttt taatcagacg
     6841 ggccgttgtt tccatgttga ccatggtgga agtcaacagc gcactcatac cgacaataca
     6901 gccggggaat tttttcacat tttccagaaa agtttccggt gcaacattgg cgcccagatc
     6961 aatcacttcg tacccggcac cttccatcat catccgcaca aggtttttgc cgatgtcatg
     7021 gagatcgccc tggacagtgc ccagaacaaa aacaccgcgc ctcgccgctg ttccttcttc
     7081 aaagaaaggc ttcagctgtt ccagtccggc gttcattgct tttgccgcca tcagaagatc
     7141 cggaacaaac gaagtgccct ggctgaactt tttgccgata atctccatac ccggcatgag
     7201 cccttttgcc agaatttcgt tggggtcgac tcctgccgcc agtgcatcac ggaccagttc
     7261 gaaaacgcca gcctcccctt tgagatcggg cggccagggg gaattttgat caactttccc
     7321 gcgcgtcaca caggtaacaa ttttttccat cgcttccatg cagtatctcc tgtaaatagc
     7381 agggtatcag aaaagagcaa cactactatc atacctaagc agcgtgctct gtttcattta
     7441 ttcctccctt tctgccaaaa aaattcctca tcccctttca ggcatcactt caggaagagt
     7501 gaaagagaca caaaaaaccg ccgcccctga gaatacacag gcagaacggg acggcggcca
     7561 ctgatgaggt caaaggaggg agggagacat gcggcaatcg gtaatttgca ggtattcttg
     7621 gggaaaggag tgacgtgagg cagaaggatt cgtcaacacc gttggagcat atctccctcc
     7681 cggggaggaa cagaaggaca aaggagaaaa atctggaagc gggtcaaacc tgaaacaccg
     7741 ttctacaggt attcttgggg aaaggagtga cgtgaggcag aacaaggcac ggtgccggtc
     7801 tgacccgcca ggagtgacaa ggaagtatgt ttcggaaacg gcggtattca ttctttagct
     7861 cctaccgcaa gcacttccgt aaaagaagtt tcgacatgac ctgagttctg cacgggaagc
     7921 acggtgacat gcagcacgga gagatcccgg ttttgcagtc cttcaggcat cctgatctca
     7981 aaagtattgc gatgcttctc ctgaaacaca tgaggaataa tggtgtattt ggaaacaggc
     8041 cgcatggcgt tatcggaaat gtacaccgta taggccgcat gtccggcaag ataaggatcc
     8101 ggcacccagc gcagtaacag ggctttaaca ttacgcgacc gtgtcttcac gacgaaaacc
     8161 tgctctccgg taagaggact tgactccggg ggcgccggca gaatactcgc ccggaaaaaa
     8221 cgctccgcct gaatatcagg tgtaagggcg cgcgcctctt ccacaaaacg atcaacatcg
     8281 gcaagagagg caatgcgcat tccggaatct ttctggaaaa cagcctgctt gccccgggtc
     8341 agacgagcaa aagaatgatc gttttccaca agcacatcgc cgttcactac actcaccacg
     8401 gttcggctgt cttcaatgcc gacctgaaaa gaagtgccca tcaccgtgat acacccgtga
     8461 ggggttgtga tggtaaaacg tcgcgaatcc cggaacacat ccagaaacat acgaccccga
     8521 tccataagga tctgccggtc actggtaatg cagacagacg aattcccgta cactgccaga
     8581 tgcgtgggac ctttcaaagc caaaagcatc tgaccgttct cggccgtatc cagagtgtct
     8641 ccggctgcca gaagcgcccc cgctttaaga tgactgctcc gggaagatac cgcattggca
     8701 aggtacgctg tacccgaggt ataggttacc acgccgacag agtccgcttg ctcgtttatc
     8761 cgttcaggcc attgcatcca gagcaacagc gccagaataa aaagaagcgc aggaacataa
     8821 acaggcatgt atcgaaaaac aggattgcga aggggctgct ttccgtaatt ggaagcagac
     8881 cgggcagagg aggtatcgcg ccaggtccgg cgggcagatt tcatttccgg cagatgcgcc
     8941 ataacaccgg tgaccagatc aacggtacgg cgatcccgat gaagtgtctc gtaaagatgt
     9001 gccgcacacg cccgtgcccg ttgcagctcc tgccggcatg acgggcagac ctcgacatgc
     9061 tcttccagca acagccgttc gacacgcccc agctcaccgt ccaggtaagt ttgcagcatg
     9121 tcttctattt ggttgcacga cgccacggaa taataccttc ttcagcctgt ttctgctgcg
     9181 ccgtcaattc ctgacgcagc aactttcccg cccgattcaa aattccacgt atttcatcat
     9241 aagaggagcc tgtaaaacgg cagatctctt tatagctcat ttcctgaaca tagcgcagta
     9301 ccaccggcag ctggtaccgt tccggcagct tctggatcgc acgatgaacc agtgcgtaat
     9361 catcggcctc aactcccggc gctgctgctt cttccgcctg cgcttcccgg gcacgccgat
     9421 ggggcaatgg tgtcgcgtac ccctcatggc ggggcgcaaa acggcgaagc cagtcggcag
     9481 cggtacgcgt tgtaatcact ttcagccagg caccgaaatg ctcctgatct ttcagctgat
     9541 gaagattccg gtatgctttc cagaaggctt cctgagcaat gtcttccgct cccccatagc
     9601 gcccggcata gtagaacgcc gttgcatata cagcatcctg ataccgctgt accagtgctg
     9661 cataggcatc ggtatcgccc tgaagactct tgcgaacaag ttctccctct gtgcgatgca
     9721 tttcttcttt cccggtaagt agtgcctgcc tgctcagcgc aatgtgtacg aatcgcctgc
     9781 gatccagccg ccccgacaca ttcttcaact ttctataagg taagactggt ataattttag
     9841 tttctgcaca ttatgctcca ttatggagca ccgcaccctc ttatatcaag gagcgcaacg
     9901 cattttactg agaaaattaa aaaaaacaag cccggcgcag aaaaagtaac ttccaggcgg
     9961 gaaaacacca atgcctttgt gatagattca aagaggcaga aggtacgaaa caattgcaga
    10021 aaggacgcac gcaatggtgt cagacaggtt gctgatcttt gatatagacg gtacgctgct
    10081 tcagacggat accgtaacag tgcctgccgt tcaacaggca ttcagagatt acgggcttcc
    10141 ggaaccggat aaagaagcca tttgcttctt cttcggaaag cctgtggaag attatgaagc
    10201 ctggatggcg tcactctgtg ctgaaggcaa ggctgaggaa gtggtcagcc atgccaacgc
    10261 gctggagctg cagtgcatnn nnnnnnnnag tgcatgcaca cctgcggact cctctatccg
    10321 aaagctctgg aaaccgtaca agccctccat gaccggggat accagatggc gatctgctcc
    10381 aacggaccag aaccctacgt ggcagccttt gttgaagcgc acgggatggc tccgtttttc
    10441 tctaccgttt atgcccgggg cacacgctac cgcgataaaa cagagatgac aggtcttatc
    10501 ctggagcagc atcgacccgc tgcgtttgcg gtgatcggag atcggcacga cgatattgaa
    10561 gcagcccttg cccacggcgg gatcggcatc gccgccgctt atggtttcgg cagtgcacct
    10621 gaatatgaac aggctgagcg ggtactcgcc agcatcgctg aactgcccga atgcctgaat
    10681 gccctatggc attgctgatt cagcagtcag atccgtcttt cgcaagcggc acagcttctt
    10741 caatcagcat caccgggata tcgtcgcgga cgatgtagag cacggcacca tcttccctga
    10801 tcagaccttc ctggatcaga tcagtcaccg tatctccggc gcggtttttc aaagtccctg
    10861 caaccacttc cgcattcagg gcatcaagta cttcaggtgc tgccggatgc agcggtgttt
    10921 tgttctccgg gcagaccagg atttttaaaa gttccggatt taccattgca gttctttatt
    10981 ctttcatcgc agttgcaaag acactcaggg cggtgtaatt tttccaagca cgacagattc
    11041 cgatgctcct gttgcctgcg gcacattggc gggcgttcca aaaatcgttt cattgccgag
    11101 aatcgcaaaa gcaatggctt cccgggcatc ataggaaata ccgacacggt cgctgatgaa
    11161 aattttcatc tcaggaagtt cttgctgcag gcgggtcata attgtgttgt tcatggcacc
    11221 gccgccgccg acaatcatct gggtcgcctc ataattgggc gcaatataat ttctgtaggc
    11281 atcggcaatg ctgcgaaccg tggcttcagt gaccgtagcg accagatctt cgtaggagtg
    11341 gttccggcgg ctggcaagag catcgcggag ataagcctcc agcccgaaac gctctcttcc
    11401 tgtgctcttg ggcggttctt tctggaagta actgtcgctg agcagatatt caagaaactc
    11461 gtcaatcact ttgccacgcc gtgctgcatc gccattttcg tcaaaggcac gggatccccg
    11521 ggacagcagc cggacagtcg catcaatggc gatattgccg ggaccggtat caaaggcgag
    11581 tatgtcttca aattttgggg ttacaacggt gaagtttgca atcccgccaa tattcagaca
    11641 cacaatattt ttttcttcgc gctgaaaaag caaccagtcc gcataaggta ccagcggggc
    11701 accctgaccg cctgcagcca tatcccgaac ccgaaaatcg gaaataacgg gcagctttgt
    11761 ctgatgcgca ataatggcgg gttccccgat ctgcagggta cccacacggt cattgccgga
    11821 gccgggcgga taatggccaa ctgtatgccc atgcgcggcg ataaagtcca cctccacctc
    11881 atggtcatca gcctgatcca gcatggcaag ggatgcctcg gcgagaagct gccccaactc
    11941 aaaattgaga aggcacactt ccttggcact catatgctct acaagcagtt tgttctggaa
    12001 atccgttgta tacggaaagg tctggtgagc cagcagttta atggcaagcc ccggtccggt
    12061 tcccttgatt cgaaccagaa cagcattaac cccatcacag gaagtaccgg acatgagccc
    12121 gatggcaaaa cgggattttt tcgcacgaat cgcttccagg ttcatcagcg tgtcctttct
    12181 atcacggcgc cggaaaaata atgcgccaca atatctctat aatcaagccc cgcggcagcc
    12241 ataccgcgtg ctccatgttg acaaagtcca acgccatgtc cccgaccgcc gccgtaaacc
    12301 ttccaatggg cgggcgtctt tcccggggga tccgccttta ttataatcat ggcactgggt
    12361 aatcctccaa atgcctgacg aattgccagt tcccgttcaa tagtcactgt tgaaagtgta
    12421 ccaactattg taacggattt taaccgtcca cttacacctc gcaccccttc ggtgacagac
    12481 aagactgttc ccactttata ttttttatta atcagagccg acaattcatc ggttgaaaaa
    12541 cgacgctgcc agcgaaaact ctgctgatca ccttcacacc aggcacgggg cgcgctctgc
    12601 agccactgaa gcatattctt cgcaggtgtg acagatccgc cgttctcacg caaatcgctt
    12661 ccgccacgta ggagcctgtc aggcgggccg ctccagactg tatcgttgtt ttcagtccag
    12721 ccaccgcagc aagaggaaaa tacggcaacg gcaaaacggt tatcaaaaga aagtgcttcc
    12781 cccgctgtct cccgtattgc ccgatccgag gaaggatgaa actccccggc accccagtag
    12841 gcgcggcagc tttcgagtgt ggtgaagtca aagccctgca gtttatattt gcctgcgaga
    12901 ctggcgtaaa tctcgctgcg tgccaccagc gcctgagctt tcaatgcttc cagcggccat
    12961 tgcaccggca tctccgccgg aacaacaccg cgcagatatt cttccaccgg cagtgtcccg
    13021 aaaatctcaa tggtatcttc tgcacctata ccgatttcca gcggcggcgc ataaaggcag
    13081 tttcgttttt tttccttcca gaaagtgccg gacacgtcca ctatttccac aacaccttcg
    13141 caatcgagaa aaagaggagc ggcaaaaaga gaagaggctt tcccgcgggg tccctgcagg
    13201 gaaaaagaag cggttcccgg ggcaatcctt tccggtcgaa tccagggagc gacgctgccc
    13261 gttttcaaac ggtcggcaag ggctttcgct tccgatttct ccttacagcg ggcaacagaa
    13321 acccagtgcc ggcgattatc aagcgtacga cctgaaggca ggcggtaaag cagaccgaag
    13381 gtaatcacct gcggagtaaa ttttttttcc tgccattcac ggagacaggc ttccttttcc
    13441 tgaacctccg caggcgcaaa agttcgtgta aatacgtggt aatgctgcgt cgcgccccgc
    13501 cctttctcca gtgtaagtgt ataggcgcca ggtgccagag aatagagcgt gtcgccggat
    13561 tcaagcgttc ctttctcctg caaacggaat gtgaattttt ctttcatctc agcataacgg
    13621 atccgcgcct tcacttccag gggatttact gcttcttcag cggaagaaca cagcatcgca
    13681 aggcagctca gcgcaaagaa agcaaagact gctctcagga cagaaacgct tcttttctta
    13741 tacggcttat tgctcactat acaaagaggg ctactcataa aggctccgga gacacggctg
    13801 tttcggctat ctcactataa cacagccaca ggggcatacg ctttatttac atccgctgcg
    13861 aagtactgat aaagaacaag gggtactgcc tgcatacttc ccctgcatct tccgcttttt
    13921 ctttcatttg tgttgttcca acttgacttc atttatttga tatgctacca tttgttagca
    13981 ctcatccatg tcgagtgcta attcccgccc cggaacggat ccggtatgtt tcaaaaattc
    14041 atgctatgaa ggagattaaa ccatggcaaa acaattactt tttgatcagg aagcacgtat
    14101 tgcactgctg agcggtgttg accagctcgc cgatgccgtc aaagccactt tgggtcccaa
    14161 gggcaaaaat gtgttgttgg agaagtcctt cggtgccccc aagatcacca aagacggcgt
    14221 tactgtcgct aaagaagtgg aactggaaga gccctatgcc aacatgggtg cccgtatgct
    14281 ccggcaggcg gcaagcaaaa caaatgatgt cgccggcgac ggtacgacaa ccgccaccgt
    14341 tctcgctcag gcacttgtcc gcgaaggcat gcgtcatgta acagccggtg ccaacccggc
    14401 gcatctgaaa cgcggtcttg acaaagccgc tgaagtggca ctggatgcca ttaaagccgc
    14461 cgcaaaaagt attaaaaatc aggatgaaat ccggcaggtc gctattgtct ccgccaacgg
    14521 cgatacaagc atcggtgaca ttattgcgga agcgattgca aaagtcggcc aggacggtgt
    14581 catcacggtt gaagagggca aagggtttgc tacggagatg gaacaggttg acggcatgca
    14641 gtttgaccgc ggctaccttt ccccctattt cgtaaccaac cgggaaacca tgcaggtcgt
    14701 gctggatgac ccgtatatcc tctgttacga gaaaaaaatc agcaatatca gcgaaatgct
    14761 ccctgttctg cagaaaattg cccaggcagg aaaaggtctt ctgattattg ccgaagatat
    14821 tgaaggggaa gcgctggcaa cgctggtggt taaccgcctt cgcggcatcc tcaacgttgc
    14881 agcagtcaaa gcgccggcat tcggtgacag acgcaaagaa atcctgcgcg acctggctat
    14941 tcttacaggc gggcagttta tcagcgaaga tctgggcatg aaactggaaa atgtggagct
    15001 gaaccagctg ggtaccgcca agcgcattga aattaccaaa gatcatacca cggtaattca
    15061 gggcggtggc gacaaaaaag agatcgcagg aagagttgat cagatccgta aaggcattga
    15121 aacaacaact tccgactacg acagggaaaa actccaggaa cgtctggcga agcttgcagg
    15181 cggtgtggct gtcattcaga tcggtgccgc cagtgaagtg gagctgaagg aaaagaaaga
    15241 tcgcgcggat gatgcgctca atgccacccg cgccgccatt gaagaaggta ttgtcgcagg
    15301 cggtggcgta gccctgatcc gtgcgcggaa aagcgtactt gacctggatc tgcagggcga
    15361 tgaaaaaacc ggtgctctca tgcttgcaaa agcaatgacc gagcctctgg caatcatcgc
    15421 acagaatgca gggtttgaag gtgctgtaaa agtaaatgaa gtttcggtgg caaaaggaaa
    15481 catcggattc aatgccgcca caggtgtcat ggaagacctc gtgaaagcgg gcatcatcga
    15541 cccggcaaaa gttgtacgca ccgccctgca aaatgccgtc agtgccgccg gtatgattat
    15601 ctccacggaa tgcctcatca ccgacgctcc taaaaaagac gatgaaaaat aatttccctt
    15661 cgcaataacc tgtgtacgcc cgatcttgca ttggtcgggc gtactttatt ataattcgaa
    15721 agtaaacact atttagcggg caaaaatgcg gggcatcttt aaagggtaac acctcgcgga
    15781 aagcgcactt gaatgccggg gattttgcgc agatgttttt tcagcgtcag cgcattctga
    15841 acagaaaaaa gccttacctc atatagagtt atttcgcagt ttatttatta ttcagctcaa
    15901 aaagtaattt ctttttcatt ttacttgatt tttaattgtg aatactgtat aattctcgtt
    15961 gtgagttctt agttggcttg gaaataccca ggggcaacat catggaccag ttcgaagaag
    16021 gacaaaaaat agcgagccgc tacatcattt tgcggaagct gggtcgtggc ggaatggggg
    16081 aagtgtatct ggtcgaagac accacatgcg gtgaacagcg tgcgctaaaa acactgcgct
    16141 ccaagtatct caaaagcaat catgccaagg cacggtttgt ccgggaagca actacagccc
    16201 gccagctcac ccatcccggc attgttacca tttacgatac cggaaactgg gaagacaccc
    16261 ttttctatac gatggactat gtggacggaa agtcgctccg tcaatggatg catcagtggg
    16321 ggcgctttga catcggctcc actgtccgga ttctgtgtct ggtggcggac gccctggttc
    16381 acgcacatcg gctcacggtc catcgcgatc tttcccctga gaacattatg gttctagcag
    16441 atggttccat ccgtattctg gatttcggtc tggcaaaatc ggacgacaag ttcaaaaatc
    16501 tgacaatggt cggtgtcaac ctgggcaaga tagcgtatat tgctccggaa cagcatatgg
    16561 atgcgtcccg ggtcgacaaa cgggcagaca tttatcctct gggcatcatg ttttttgaaa
    16621 tgctcaccgg acgtcgcccg gctctgggcg acaaaatttc ccgcatttgc cccgaacttc
    16681 cccgggagac cgatgtcttt cttgccaaag ccatggcacg caatcccaac gaccgtttcc
    16741 cctcggcgca agtgtttcag tctgaattgc tggcactgta ccaacgtgtc cgtgactggg
    16801 agataaagcg caatactttc tttatgcgca tctggattgc cgtaacaaat ctgtttcagc
    16861 gaaaacaggc gcgtcctcct ctttgagtcg ccaacaccac cgccacaaaa accgattccc
    16921 cctctctttc ccctgccttc atgcttctat gttcgtgtgc gtttatctat atgccgggtg
    16981 cattttcaag caaagcactg aaaagaaaaa cggcaagagt tcctgccaca aaaaaaccga
    17041 tgtaccgcat aacaatctct cctgtgttta ccatgattaa caataggtgc gcatggtaaa
    17101 ttccgagccg tcatagcgaa cataggaacg gtggatgggc cagtcacccg tattgatgta
    17161 acaaccggag aaacctgcag atccatacgt ctccaataca ggcgcatgcg catggccgca
    17221 gataaccagt gtttct
//
LOCUS       DAOPWB010000069        17088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3399_length_17088_cov_5.703047, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000069 DAOPWB010000000
VERSION     DAOPWB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 17088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17088
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3399_length_17088_cov_5.703047"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="PKN92_07800"
     CDS             complement(<1..1457)
                     /locus_tag="PKN92_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNZ48734.1"
                     /translation="MISSFALLIGRMILWCMPAAIGLTALESIGQTLTAGYLPLSFSD
                     YFLQFSNSSLLLFGWLCLFSAVLSFIFVWLGGIRPGIVRLFLFLFFFLFTYLIIVLDI
                     LFTYTDQYALHWHTIMRYFGVFSFLVAGAASLILGKISRNRQWSVPPRLFFAFTLFLF
                     LLSLCFQAKPVFFDTRDNTLFWGGALLVILTSAVGLTLFAKSTRRLNLLLFLLLVLIH
                     GSAGCIRFQKASTPLPDPAAGRQIAATVPSKQILLITVDTLRQDALGLYGNLRAKTPH
                     LDTLGKDSLVFTRAYSPAPWTYPAVASFLTGVSPRVHGMTNGKTSLPPAIPTLAESMQ
                     QAGYRSCAIGTNAFLLPRSGLDRGFDEYFWFPAQAVEMPAFTTGLSHNLLLLGGRKRA
                     NAAVLTDHAIDWLQQHRNEPFFLWVHYFDPHVPYEPPENFLPEDPELRKAGSSFSDVR
                     AARAGSIARTAREREWIRALYQGDVSYTDEQIGRLL"
     gene            complement(1546..2214)
                     /locus_tag="PKN92_07805"
     CDS             complement(1546..2214)
                     /locus_tag="PKN92_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ48735.1"
                     /translation="MKVLNFSLSLFVVALFSACAAMSDGAECTKAPEEGYVSLFNGKD
                     LSGWMGDTAGYVVQKGAMVCKPGGNIFTEKEFSDFSFRFEFKMTPGANNGVGIRAPLT
                     GNPSYTGMEIQILDDRHPKYNEIHEWQAHGSIYGIVPAKRDHLKPVGEWNEEEIIAKG
                     RRVTVILNGETIVDADLDEATKDGTLDGQEHPGVKNASGHIGFLGHGDILKFRNIRIK
                     ELTD"
     gene            2467..4185
                     /locus_tag="PKN92_07810"
     CDS             2467..4185
                     /locus_tag="PKN92_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNZ48736.1"
                     /translation="MSRISYRMIAWLLVFFLSLSSVACSSFIFEGDVPVTMSDGVVLP
                     VNIARPADDGKYPVILLRTPYGKPGEDNGDMKSYAQAGYVMVVQDCRGTGKAGGEWEP
                     FLHDPQDGLETQAWIAEQPWCDGRIGLSGGSYVGWTQWASLPGARPEVRCAVPVVPFA
                     ECYPDLAYQGGAFQLALLMGWGAMMGGAGLTEESAQEAFRYLPLNTFGNQFKKPVPYL
                     NAWVEHADPSGDYWRLRGINGRYSEIEAAVLNIGGWYDIFSKPVLDLCTRVRQESTSE
                     FARKNQHVVVGPWAHGVGARKVGALDFGADAETKIHKLQFDWFEHWLKDKDTGVEAWP
                     PCRIFVMGENRWRNENEWPLARTVYTPFYLHSSGSANTRSGDGTLSKELPSEEAADRF
                     VYDPENPVPTAGGNNLFGAPAGPFDQSALEERADVLVYTSEVLEEALEVTGPVHMILH
                     AASSAPDTDFTAKLLDVHPDGKAYNLCDGIVRARFRRGMDQELFLTPGEAVEMDIDLW
                     VTSNLFLPGHRIRVEISSSNFPRFDRNPNSGLPFGTDTVLHPAEQTVFHDSLRPSHIL
                     LPVIPR"
     gene            4236..6071
                     /locus_tag="PKN92_07815"
     CDS             4236..6071
                     /locus_tag="PKN92_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNZ48737.1"
                     /translation="MKTFLYASFFAPVFVFALSFCSAAEENALPREPYKTALITRWGE
                     GVTAENAWREYPRPQMVRENWTNLNGLWDYAVTGKEQTEAPSAMDGQILVPFAIESRL
                     SGVQRLLQPEEALWYKRTVVLSPVPGKRTLLHFEAVDYDCELRVNGIIQGTHRGGTPF
                     SFDITGALKDGENELLLRVEDPTDNWQIRGKQVLDPHGIWYTRVSGIWQTVWLEEVPE
                     RYISSLLLTTDAAKGTIHLSAGLVGAPQNAALSVSVRSGADTIASGTGPASGITIPVH
                     NPVLWTPDTPHLYDLEIALLSDGGEAVDRVSSYAGIRTVGKIRDANGHLRFTLNGEIL
                     FHWGPLDQGWWPDGLLTPPSDEAMRSDVEFLKKAGFNMIRKHIKMEPRRYYYACDQLG
                     MMVWQDQPSAGHGPKWTFLKENPEDAEWPEAQHLQYMTELEGMVIALINHPSIVVWVP
                     FNEAWGQHRTMEVGAWMAARDTSRLVNIASGGNFWPVGDIVDFHKYPDPGFPFDENRY
                     REFIKVVGEFGGHGYPVDQHLWNPDQKNWGYGNLPKNKGEYEERYARTLDGLNELRKA
                     GIAAGVYTQTTDVEIEINGLLTYDRAMEKIPAEKLHELSRILFED"
     gene            complement(6148..7275)
                     /locus_tag="PKN92_07820"
     CDS             complement(6148..7275)
                     /locus_tag="PKN92_07820"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNZ48738.1"
                     /translation="MFKYLPLTLLLLLPATGCHAVPEGSFAEGLSCCRELFNTSMKEG
                     VSCYRIPALAAASDGTLLAVIDERVADGSDLGTNRNINLVLRRSSDNGDSWSDLESLV
                     DYPDGRSASDPSLLVDEITGQIFLFYNFMDHDKAPGEYRLHVMKSSDNGSTWSEAEDI
                     TGQITKEDWQHDFKFITSGLGTQTRSGLLLHTLVNLKRGLHLFASRDHGKSWTLIDTP
                     IRPADESKVVELDDGRWLINSRVNKAGKRFTHFSSDEGKSWESRAEKTLVDPGCNGAL
                     LRCTQKSKGAAKNRLLFSNAMDRKERRNLAVRISYDEGESWSSGKVIYSGSAAYSTMA
                     LLKNGDIGIFFEKDDYSNNVFVRFSLNWLTEGNDPGESLER"
     gene            complement(7304..10357)
                     /locus_tag="PKN92_07825"
     CDS             complement(7304..10357)
                     /locus_tag="PKN92_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNZ48739.1"
                     /translation="MKKFSFLCLTAMTFTALFSLDCAALDTVTVRTKEENAPISPYVY
                     GQFIEHLGRCIYGGIWAEMLEDRKFFFPVDGNREGWGMHQGNETSWEGDGKPYEILEA
                     SPWQIIGPPEGVFMSRENPFCGEQDCAVILDKEGGLRGIAHPRLEVIAGNTYEGYVYL
                     RCEGTVDQAEAVLQWGPDTGQEAACPLEGITGEFRRFPFQLTAGGSDTHARLAFRFSG
                     KGRIFIAAPSLMPDNHVDGFRPDTLALMRKLDAPIYRWPGGNFVSGYNWRDGIGDRDK
                     RPPRKNPAWTGIEHNDVGIHEFMRLCELIHTEPFIALNTGLGDIESAVAEVAYITGSA
                     NSEEGKKRAANGRKEPWKAVWWAVGNEMYGFWQLGHMPLEEYVKKHNAVVDAIRHVSP
                     EAKCVGVGNAGPWSETMLARCSNHMELISEHLYWQQVDNVVDHTWLAINDVERLANDH
                     RRYRETIAGLKEKDVRIAFDEWNYWYGPNQNGELGVRYQMRDALGISGALHAMFRNSD
                     LFFMANYAQTVNVLGAIKTTRTRAFLESTGQALVLYRHHFGTLPVPVQHELGGVDIAA
                     AWAADHSALTVAVINANDADIRFQLNLKGLRLPESIRGWQIQNDNPDSLNEADTPDTI
                     CIKEVQVPAENSSISVPRYSVTLLRIPNADDPAVIYWEKEERRWSRKRINDWYANGPY
                     PAGANFVPSTASNQFEMWQEDTWDPETIDRELGWAAELGFNVMRVFLHDMLWRAEGTA
                     FEKRIDEYLAIADKHGIRTMLTLLDSVWDPFPELGRQKEPVPGVHNSRWVQAPHIDIQ
                     KNPEGYDAALKPYVTSVISRFKDDPRIFAWDLLNEPANPVPQYQPTEGWSPQEKEAAH
                     LILVDKLFDWAREVNPSQPLTVGIWVAPGNRINPANQLDKLMAERSDLITFHSYQNVM
                     EMKKAVEWLQQTDRPLVCTEYMSRSTGCTFQNVLPYLKEQNIGAVNWGLVSGRSQTIY
                     PWHSWEKPFDKEPDPWFHDVFRKDGTPYDEEEAAVIRAVLKKEKP"
     gene            10560..11792
                     /locus_tag="PKN92_07830"
     CDS             10560..11792
                     /locus_tag="PKN92_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNZ48740.1"
                     /translation="MLNEQQTWACADWVMTQVRQVIENFGPRAPGSEEERRAQEYIRK
                     EMKAFCDGDVILEPFPLAAKAFFALAAVTAFFFTLSALCWHMHPFCSVVLDGVALAVL
                     YFQLFRYRLFLDPFFPKKESCNVYGRIRPSGEIRRRVILAGHVDSACEWRFLYWMPRF
                     FRPLMIALFSGLALLTLLHAGGSVAWILGGEAWTVASRIVLLQWVLSPLLLVGLFFSD
                     LKQPVPGANDNLTGVFSCLAIGRALKQGGLSLKHTELVLVSMGSEEAGLRGAKVFARK
                     HKEDFSDVQTLFIGLETLRDPEYLTIYDRDMNGMVRHDEQVCSLLKEAGKICGLDLPF
                     GSLFLGSSDAAAFTQAGWQAGLLAAMDPAPPAYYHTRLDNWDNMSRDCLKTAIQVTCA
                     ALKLFDEEAEEPMEFSDN"
     gene            complement(12031..12210)
                     /locus_tag="PKN92_07835"
     CDS             complement(12031..12210)
                     /locus_tag="PKN92_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48741.1"
                     /translation="MNPSKTPAKSGTRSAFRRKSQAPYDALPPESDPDGAADARQEQA
                     IAEKGIRVLQAWLEG"
     gene            12635..14608
                     /locus_tag="PKN92_07840"
     CDS             12635..14608
                     /locus_tag="PKN92_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48742.1"
                     /translation="MPAGTELVFISVTGLPRAWFNLGYVSPAPSTLVAETDGMAALDL
                     THYLYYHRVFPGHDDQEIEVLLGIIFGDHESFEITVTVVCVRGADMSNFPGDGSLFTG
                     TVYEINDGSTEEEVSINPPEILKRGDMPLFIAGAQGTLGDVLDGDTVSASSVKGTFLS
                     SAVGYARVGARTTSIELGWQANVTQYSAFCIPLHSRSFTAVQLNIMPVSAVQAGAMWS
                     ADWSEFGETRWQETGTLVAQQPLGKWPFKFFAVPEHSVPESIIDIQPGDPTVLYAAYH
                     KRGTPEVLKTEYYAGNAVSDTGYEVAFDGTVPWNAELALLLISTRSEEKIAVNYSSPS
                     GLSMYRKSEYSLPSLVKSFGPSEEAGISTSLYCIKRPQLNPGGIYDEMIILAPEEGNE
                     AVPDTITLLFLRGVDLDAFSNPWEGTSLFPQVTYESGTATTIAREVNSWSLPPGGAMV
                     LSILNGTASVGGLTDSLQQLLWAGGTPDNSLTTSGSALLMLPGDNNAELQWQASTGEE
                     GWDLLQIILPATGTGSLKVSCSDADQAPENPTWRIDNEPRRSSGYQDNGISAGWHWLE
                     FSDPDPYTLQTPPGRWIYIAAGTSLSESGRYALSGYPHPVYQFSFTALTNDTGNLSLN
                     HTLIPELTGDGGFCFRRLDNCNSCRLQRGYCGG"
     gene            14535..>17088
                     /locus_tag="PKN92_07845"
     CDS             14535..>17088
                     /locus_tag="PKN92_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48743.1"
                     /translation="MADSVSGDLTTVILAGCSGDTVEVEQGAGKTFTPWARGGGLGGS
                     APGEISVFFRKNHSINRESLWDFKTGLPNRAVSGTVTFFTNVDAAAVRAGLNAAGGGG
                     SNKPGQTVNLLNPLTHPSPYDMLYGAIQSQNIGQPRPNLNISKKLTLLSNTPAYRDKD
                     SIIGQMLGYTCLPTPEKPEAEQGPAFIVERDWGSVEALLPHASGNVTVTITPPELAAR
                     PEMKWSIHGGDGDKSSGETSSALCGHQKIKVPQPLDYKLLNKEDLIVEVAPGGSLQVT
                     LNYQILPPTSFSIIIAPTKAADNGAAWRIKDHNPPDSLRHDSGYKYNDITAEDVERGY
                     VTVEFREANHPTDGKYPAPEDRTFAITAGSTNTSIVSYYQYGYVRFRVEPPEAAAMGP
                     LWRIRGDDEGDGWRTYSPDEIIRLPILRRGIEFSDLEGWQTPDLDIHVQYSEIDDAQS
                     YVVQYQPYGLPRVRGRSSWNGTPGGATVNVKTSIPKAPSPQICLLFLTWQSSANTNSE
                     PTQVQLHTTNFNRLVISPEGKVRTAIYFIEGGMGYTSPAGDDLQIIFPSSAVPDALQV
                     ECIFLDDVDGGSPWYSASRSGASPAVPESYLEYPWLPATMFSSLSTIADSFSPDFDSA
                     SDISDPALIGYATFDGASGGTALISGEMTGTRPRISWNCEGDWALASIAIPAAQGDVQ
                     VNIHPAAAVTDGVKWNIKPKYLSGTPSGGTRKAALGLQTILPKDHNGWRLDPAYDNTV
                     EVQRTGTAVGELHLLPAPRAGIRVNIFPVEAVAAGAKWRVSGMGPDEWQESGTVWTGL
                     KDLDRYYLPELSMIDGWDRPLASVNPVTVSSGEITEVSVLYTRRGALRVTLMK"
BASE COUNT         3846 a         4466 c         4735 g         4041 t
ORIGIN      
        1 agaagcctgc cgatctgctc atccgtatag ctgacatcgc cctgataaag ggcacggatc
       61 cattcgcgtt cgcgggcagt gcgggcaatg cttccggcac gggcggcacg gacatctgaa
      121 aatgaacttc ctgctttccg cagctccgga tcctccggca ggaagttttc gggcggttcg
      181 tagggtacat gagggtcaaa ataatgaacc caaagaaaaa atggctcgtt ccggtgctgt
      241 tgcagccagt cgatggcatg atcggtcagt acggctgcat tggcgcgttt ccgtcctccc
      301 aggaggagca gattatggga gagtcctgtg gtgaaagcgg gcatttccac ggcctgcgcc
      361 ggaaaccaga aatattcgtc aaagccccgg tcaagcccgc ttcggggaag gagaaaagcg
      421 ttggtaccga tggcgcagct gcggtagcct gcctgctgca tggattctgc cagcgtgggg
      481 attgcgggag gcagcgaagt ttttccattt gtcatgccgt gaacccgcgg ggatactccg
      541 gtaagaaagg aagccaccgc cggataggtc cagggcgcag gagaatatgc tctggtgaat
      601 acaagactgt ctttgccgag tgtgtcgagg tgcggcgttt ttgcccgaag attgccgtac
      661 agtcccagcg cgtcctggcg cagcgtgtcc actgtgatga ggagtatttg tttggatggc
      721 accgttgctg caatctgccg ccctgcagca gggtcgggaa gaggcgttga tgctttctga
      781 aaacggatac agcccgcact tccgtgaatc aaaaccagca gcagaaaaag gagcagattc
      841 agccgcctgg tggatttggc aaagagcgtt aaacccacag cggaagtcag tatcacaagc
      901 agggcgccgc cccaaaaaag cgtattgtct ctggtatcaa aaaatacagg ttttgcctga
      961 aaacacaggg acaacagaaa aagaaagagg gtgaaggcaa aaaaaagccg cggtggaaca
     1021 gaccactgcc ggttccggct gattttcccc agaataagac ttgccgcacc ggcgaccaga
     1081 aaggaaaata cgccgaaata ccgcatgatc gtgtgccagt gaagcgcata ctgatccgta
     1141 taggtaaaca gaatatccag tacgatgatc agataggtaa aaagaaaaaa gaataaaaag
     1201 agaaacagcc ggacaattcc cggacggatg ccgccgagcc acacaaaaat aaaagaaaga
     1261 actgccgaaa aaaggcagag ccagccaaaa agcagcaggg aactgttgga aaactgaaga
     1321 aaataatcgg aaaaggaaag agggagataa ccggcagtca gggtctgtcc aatggattcc
     1381 agggcagtca gccctattgc cgcaggcata caccagagaa tcatccggcc gatcaataag
     1441 gcgaaagagg atatcactgc ggtgctcctt ccttaggcag tatgacaggc aggagtttca
     1501 gaggctgcac tcctgcctgt tcgggacgct tccggcggat gccgttcagt ccgtcagttc
     1561 tttaatgcgg atattacgga acttcagaat atcgccatga cccagaaagc cgatatgccc
     1621 ggaagcgttt ttcacgccgg gatgttcctg cccgtccaga gttccgtctt tggtcgcttc
     1681 atccagatcg gcatccacaa tggtttcgcc gttgaggata acagtgaccc ggcggccttt
     1741 cgcaataatt tcttcctcgt tccattcgcc cacgggtttg agatggtccc gcttggcggg
     1801 gacgataccg tagatggaac catgagcctg ccattcatga atttcgttgt atttggggtg
     1861 ccggtcatcc aggatctgaa tttccatacc cgtataggaa ggattgccgg tgagcggagc
     1921 gcggatgccg acgccattgt tggcgcctgg cgtcattttg aattcaaagc ggaacgagaa
     1981 gtcggaaaac tctttttccg tgaaaatgtt gccgcccggt ttgcagacca ttgccccttt
     2041 ttgcacaacg tatcccgctg tatcgcccat ccagccactc aggtctttac cgttaaacaa
     2101 agaaacatat ccctcttcag gtgcttttgt acattctgca ccgtcggaca tggcggcgca
     2161 ggcactgaag agagccacta caaaaagcga aagtgaaaaa ttcaaaacct tcatgaatac
     2221 cgattccttt catcccttac agggctattc gtgcttgaca gaagaaagaa acgtctgatt
     2281 tcataacgaa atacagacag gtgaaagcta ttttattatg ccacgctccg acgatggctg
     2341 caaactgccg gcgacggcgc cgccaatgaa tggggatgcc tggcatgttt tgtggctttt
     2401 tgccactttt ctgtatagaa tgatgcacac taaaagatct ctttttttca acagaaaggg
     2461 ttgatgatga gccgcatttc gtaccggatg attgcctggc tgctggtatt ttttctgtct
     2521 ttgtcctctg ttgcctgcag cagttttatt ttcgaaggcg atgtgcctgt gactatgtcg
     2581 gatggggtcg tgctgccggt gaatattgcc cggcctgccg acgatggtaa ataccccgtg
     2641 atcctgctgc gtacacccta cggaaaacct ggcgaagata acggcgacat gaaatcctat
     2701 gcgcaagcag gttatgtaat ggttgtgcag gattgcaggg gcacaggaaa agccgggggc
     2761 gaatgggagc ctttcctgca tgatccgcag gatgggcttg agacccaggc gtggattgcg
     2821 gagcagccct ggtgtgacgg gcgtatcggt ctttcaggag gctcctatgt gggctggacg
     2881 caatgggcat cactgccagg ggcgcgtcct gaagtgcgtt gtgcggtgcc tgttgtgcct
     2941 tttgcggagt gctatccgga tcttgcctat cagggcggcg cttttcagct ggcgctgctg
     3001 atgggctggg gcgccatgat gggcggtgcc ggattgacag aggaaagcgc gcaggaagcc
     3061 ttccgctatc tgcccctgaa taccttcggc aatcagttta aaaaaccggt tccttatctc
     3121 aatgcctggg tggaacatgc cgatccgtcc ggtgattact ggcggcttcg cgggattaac
     3181 ggccgttatt ccgaaattga agcggctgtg ttgaatatcg gaggatggta cgatattttc
     3241 tcaaagcctg tgctggatct ctgtacccgt gtgcgccagg aatcaacaag tgagtttgcc
     3301 cgaaagaacc agcatgtggt ggtgggtccc tgggcgcatg gcgtaggtgc ccggaaagtg
     3361 ggcgcgctgg acttcggcgc ggatgcggaa acgaagattc ataagctgca gttcgactgg
     3421 tttgaacact ggctgaaaga taaagataca ggggtggagg cgtggccgcc ctgcaggatt
     3481 tttgtgatgg gcgaaaaccg ctggcgcaat gaaaacgaat ggcccctggc gcgtacggtt
     3541 tacacacctt tttatctgca cagcagcggc agcgccaata cccggagcgg ggacggtact
     3601 ctttcaaaag aactgccttc agaggaggcg gcggatcgct ttgtatatga tccggaaaac
     3661 ccggttccta cggcaggcgg aaacaatctt ttcggtgccc ctgccgggcc ctttgatcag
     3721 tcggcgcttg aagagcgggc ggatgtcctg gtctatactt ctgaggtact ggaagaagcc
     3781 ctggaagtga ccgggccggt acatatgatt ttgcatgctg cctcttccgc tccggacaca
     3841 gacttcactg ccaagctcct ggacgtgcat ccggacggaa aagcctacaa tctgtgtgac
     3901 ggcattgttc gggcacggtt ccgccgcggt atggaccagg agctgtttct tacaccgggt
     3961 gaagccgtgg aaatggatat tgacctgtgg gtgacgagca atctgtttct gcccggacac
     4021 cgcatccgtg tggagatttc cagcagcaac ttcccccgct ttgaccgcaa tcccaacagc
     4081 ggtcttccct tcggtacgga tactgtgctg cacccggcgg agcagacggt atttcacgat
     4141 tcgctccgcc catcgcatat tctgctgccg gtgataccca gatagaagat aggctttact
     4201 cttaatctag aataaactca ggaaaggaat ctgtagtgaa aacatttctg tatgcctctt
     4261 tctttgcgcc tgtttttgtg tttgctttgt ctttctgttc tgcagcggag gagaatgctt
     4321 tgcccaggga accttataaa acagcgctga ttacacgctg gggtgaaggt gtgacagcgg
     4381 agaatgcctg gcgcgaatat ccgcgccctc agatggtgcg tgaaaactgg acgaatctca
     4441 acggactgtg ggactatgcg gttacaggaa aagagcagac ggaagcgccg tcggcaatgg
     4501 acgggcagat cctggttcct tttgccattg aatcacggct gagtggtgtg caacggctcc
     4561 tgcagcccga agaagcgctc tggtacaaac gtacggtagt gctgtcccct gttccgggca
     4621 agcggaccct gctgcacttt gaagccgtgg actatgactg cgaactgcgc gtgaatggta
     4681 tcatccaggg gacgcatcgg ggcggcacgc ccttttcctt cgacatcaca ggtgcgttaa
     4741 aagacggtga aaatgaactt ctgcttcgtg tggaggatcc gacggacaac tggcagatcc
     4801 gggggaagca ggtgctggat ccccatggta tctggtatac ccgtgtatcg ggcatctggc
     4861 agactgtatg gctggaagaa gtaccggagc gctatatcag cagccttctg ctgaccacgg
     4921 atgcggcgaa gggaacgatt catctttcag ccggtcttgt cggtgctccg cagaatgcag
     4981 ccctgtctgt cagcgtgcgc agcggcgctg atacgattgc ttccggcacc ggtccggcat
     5041 caggcattac cattccggta cataatcctg ttctgtggac accggataca ccgcatctct
     5101 atgacctgga aattgcgctt ctgtccgatg ggggcgaagc ggtcgaccgg gtcagctcct
     5161 atgccggtat ccgtacggtt ggaaaaattc gtgatgcaaa cgggcacctg cgttttacgc
     5221 tcaatggcga gatactgttt cactgggggc cgctcgacca gggctggtgg cccgacgggc
     5281 ttctgacgcc gccttcagac gaagcgatgc gttccgatgt tgagtttttg aaaaaagcgg
     5341 gattcaatat gatccgcaag cacatcaaaa tggagccccg gcgttattat tacgcctgtg
     5401 accagctggg catgatggtc tggcaggatc agcccagtgc agggcacggc cccaaatgga
     5461 cttttctgaa ggaaaatccg gaagatgccg agtggcccga agcgcagcat ctgcagtaca
     5521 tgacggagct ggagggcatg gtcattgctt tgatcaatca cccgtccatt gtggtatggg
     5581 taccctttaa tgaggcgtgg ggtcagcacc gcaccatgga agtgggtgcc tggatggcag
     5641 cccgggacac cagcagactg gtgaatattg ccagcggcgg caatttctgg ccggtgggcg
     5701 atattgtcga cttccacaaa tacccggacc ccggttttcc ttttgatgaa aatcggtacc
     5761 gtgaatttat caaggtggtc ggggaattcg gcggtcacgg ctaccccgtg gatcagcatc
     5821 tgtggaaccc cgaccagaaa aactggggct atggcaatct gccgaaaaac aaaggggaat
     5881 atgaagaacg ctatgcccgc acgctggacg gtttgaacga acttcgtaaa gcggggattg
     5941 ccgccggagt gtatacacag acaacagacg tggaaatcga gatcaacgga ctgctgacct
     6001 atgaccgtgc catggaaaag ataccagcgg aaaaactcca cgaactcagc cggatcctct
     6061 ttgaagactg acggcgcaaa ggcagtgttt ctctgctgat ctgtatgacc cggcgggtat
     6121 gccggagcgc tgcgaaaaaa aagcagctta ccgctccagt gattcccccg ggtcgtttcc
     6181 ttctgtcagc cagttgaggg aaaagcgcac aaagacattg ttgctgtaat cgtctttttc
     6241 gaaaaagatg ccgatatcac catttttcag cagtgccatg gtcgaataag cggcggatcc
     6301 cgagtaaatc actttgccgc tgctccaact ttccccttcg tcatagctga tacggacggc
     6361 aagattccgg cgttcttttc tgtccatggc gttggaaaaa aggaggcggt tcttcgccgc
     6421 accttttgat ttctgtgtac agcggaggag tgccccgtta cacccgggat caaccaatgt
     6481 tttttctgcc cggctttccc aactttttcc ctcatcggaa gagaagtggg taaaacgttt
     6541 gcctgctttg ttgaccctgc tgttgatgag ccagcgaccg tcatccagtt ccaccacttt
     6601 tgattcgtcc gcaggcctga tgggcgtatc gatcagcgtc cagcttttcc cgtgatcccg
     6661 gctcgcgaac aggtgcagcc cccgcttcag gttgacgagt gtgtgcagga gcagcccgga
     6721 ccgggtttgt gttccaagcc cggaggtgat gaatttgaag tcgtgctgcc agtcctcttt
     6781 cgtgatctgc cctgtaatat cttccgcctc gctccaggtg ctgccgttgt cgctgctttt
     6841 cattacatga aggcggtatt caccgggcgc tttgtcatga tccatgaaat tgtagaagag
     6901 aaaaatctgc cctgtgattt cgtcgaccag cagcgacggg tcggaggcgc tccggccgtc
     6961 gggataatca accagacttt ccagatccga ccagctgtcg ccgttgtcgc tgctgcggcg
     7021 cagcaccaga ttgatattcc ggttcgtgcc cagatcactt ccgtcggcaa cccgttcatc
     7081 gatgacggca agcagcgtgc cgtcggaagc ggctgccaga gcagggatgc ggtagcagct
     7141 gaccccttct ttcatggagg tattgaaaag ttcccggcag cagctgagcc cttcggcaaa
     7201 agatccctca gggacggcat gacagcccgt tgcagggagg agcaggagca gcgtcagagg
     7261 caggtattta aacatgcggt tcctttccgg gtttgagtaa aagtcagggc ttttcctttt
     7321 tcagcactgc acggatgact gcggcttcct cttcgtcata gggagttccg tcttttctga
     7381 aaacatcatg aaaccaggga tcaggttcct tgtcgaaagg cttttcccag gaatgccagg
     7441 ggtatatggt ctgggagcgg ccggagacaa gaccccagtt cacggcgccg atattctgtt
     7501 cctttaaata gggaagcaca ttctgaaagg tgcagcctgt actgcgtgac atgtattccg
     7561 tgcagaccag gggccgatcc gtctgctgga gccattccac cgcctttttc atttccatga
     7621 cattctgata actgtggaag gtgatgaggt cggagcgctc agccatcagt ttgtcgagtt
     7681 ggtttgccgg attgatacga ttaccggggg ccacccagat accgacagtg agcggctgcg
     7741 aaggattgac ttcccgtgcc cagtcaaaaa gtttgtctac cagaatgagg tgagcggctt
     7801 ctttttcctg cggcgaccac ccttcagtcg gctggtactg cggcaccgga ttggcgggtt
     7861 cgttgagcag atcccaggcg aatatacggg gatcatcctt aaagcgggaa atcacggaag
     7921 tgacataagg cttcagtgct gcgtcatagc cttccgggtt tttctggata tcgatatgcg
     7981 gcgcctgtac ccagcgcgaa ttgtgtacac cgggcaccgg ctctttctgg cggcccagct
     8041 ccgggaaagg atcccagacg ctgtccagca gggtgagcat ggttctgatg ccgtgtttat
     8101 cagcaatggc gagatactca tcgatgcgtt tttcgaaagc cgtcccttcc gctctccaca
     8161 gcatgtcatg cagaaacacg cgcatcacat tgaagcccag ttcagccgcc cagcccagtt
     8221 cgcgatcaat cgtttcagga tcccaggtgt cctcctgcca catctcaaac tggttggatg
     8281 ctgtggaagg aacaaaattg gcgccggcag ggtagggccc gttcgcatac cagtcgttga
     8341 tccgcttgcg gctccagcgc ctttcttcct tctcccaata aatcacagcg ggatcatcgg
     8401 cattggggat gcgcaggagg gtcacactgt agcggggcac agagatgctg ctgttttcag
     8461 ccggaacctg cacttcttta atgcatatgg tatcaggcgt gtccgcttca ttcaggctat
     8521 ccgggttgtc attctggatc tgccagcccc ggatggactc cggcagtcgc agccctttca
     8581 ggtttaactg aaaacggatg tccgcgtcat tggcattaat gaccgccacg gtgagtgcgg
     8641 aatgatcggc agcccaggcg gcggcaatat cgacaccgcc gagttcatgc tgaaccggta
     8701 cgggcagggt accgaagtga tggcggtaca gtaccagtgc ctgaccggtg ctttccagaa
     8761 atgcccgggt cctggtggtt ttaatggcgc ccagcacatt cacggtctgg gcatagtttg
     8821 ccataaaaaa gaggtcgctg ttgcggaaca tggcatggag ggcgccggaa attcctagag
     8881 catcacgcat ctgatagcgc acacccagct cgccgttctg gtttgggccg taccagtagt
     8941 tccactcatc aaaggcgatg cgtacatctt tttctttcag tccggcgatg gtttcccgat
     9001 accggcgatg gtcgtttgcg agccgttcca catcattgat ggcgagccag gtgtggtcta
     9061 ccacattgtc cacctgctgc cagtagagat gctcgctgat cagctccata tgattgctgc
     9121 agcgggcaag catggtttcg ctccaggggc ctgcattgcc tacacccaca catttcgctt
     9181 ccggtgatac atggcgtatg gcatccacga ccgcattgtg ctttttcaca tactcttcca
     9241 gcggcatgtg acccagctgc cagaaaccgt acatttcatt gcccactgcc caccagacag
     9301 ccttccacgg ctcttttctg ccattggcgg cccgcttttt cccttcctcc gaattcgcgc
     9361 tgccggtgat atatgccact tccgcaacag ccgattcaat atcaccgagt cctgtgttga
     9421 gcgcaatgaa gggctctgta tggatcagtt cgcaaaggcg catgaactca tggatgccca
     9481 catcgttgtg ctcaatccct gtccaggcgg gatttttgcg gggaggccgt ttatccctgt
     9541 cgccgatgcc gtcgcgccag ttgtatccgc ttacgaaatt accgcccggc cagcgataga
     9601 tgggcgcatc cagtttacgc atgagggcaa gtgtatcggg gcgaaagccg tccacatgat
     9661 tgtcgggcat gagggacgga gcggcgatga agatgcgccc tttcccggaa aaacggaaag
     9721 cgaggcgggc atgcgtatca gaaccgcccg ctgtcaattg aaagggaaag cgtctgaatt
     9781 ctccggtaat gccttccaga gggcaggcgg cttcctgccc ggtatccggt ccccattgca
     9841 gcactgcttc cgcctgatca accgtacctt cacagcggag atacacataa ccttcatagg
     9901 tatttccggc gatcacttcc agtctgggat gagcgatgcc gcgcaagccg ccttctttgt
     9961 ccagaataac agcacagtcc tgttctccgc agaaaggatt ctcccggctc atgaaaacgc
    10021 cttccggcgg tccgatgatc tgccagggag aagcctccag aatctcgtag ggctttccgt
    10081 ctccctccca ggaggtttca ttgccctgat gcatgcccca gccttcccgg ttgccgtcta
    10141 cagggaagaa aaatttccgg tcttccagca tctccgccca gatgccgcca taaatgcagc
    10201 gacccaggtg ttcaatgaac tgtccataaa cataagggga gatcggcgcg ttttcttctt
    10261 tcgtgcggac ggtaaccgta tccagtgcgg cacagtccaa agaaaaaagg gctgtaaaag
    10321 tcatggcggt aagacagaga aaagaaaact ttttcatgag caactccttt gccggacatt
    10381 tgaaggatcc tattcggaag gggactgatg ccattctatc gtttttagag caaggtcggc
    10441 aaagtatttc cgggcgtgca gttcctggtg tatacagcgg atgtgctaag atgcaggaaa
    10501 gaaagccttt cggctttttg ttacaggtgg ttttccatcc ttgactggag ggatcgggca
    10561 tgctcaatga acagcagacg tgggcttgtg cggactgggt tatgacgcag gtgcgtcagg
    10621 taattgaaaa tttcggaccc agagcgccgg gcagtgagga ggagcggcgc gctcaggaat
    10681 atatccgcaa ggaaatgaaa gcgttctgcg acggcgacgt tatactcgag ccttttcctc
    10741 tggcggcgaa agcatttttt gccctggcag ccgtgacggc ttttttcttt accctatcgg
    10801 cactatgctg gcatatgcat cctttctgtt ccgtcgtgct ggacggcgtg gcactggcgg
    10861 tgctttattt tcaactgttc cggtaccggc tttttctgga tccttttttt ccgaaaaaag
    10921 aatcctgcaa tgtatatgga cgcatccgtc cttccggaga aatccggcgg cgtgtgattc
    10981 ttgccgggca tgtggacagc gcctgtgagt ggcgttttct gtattggatg ccccgctttt
    11041 tccggccgct catgattgcg ctgttcagcg gtctggcgct gctgactctg cttcatgcgg
    11101 gcgggtcggt tgcgtggatt cttggcgggg aggcgtggac agtcgcatcc agaattgttc
    11161 ttcttcagtg ggttctgtcc cctctgcttc ttgtcggtct tttcttcagc gacttgaagc
    11221 agccggtacc cggtgccaat gacaatctga cgggcgtttt ttcctgcctg gcaataggtc
    11281 gtgcgctgaa gcagggaggg ctgtccctga agcatacgga acttgttctg gtcagtatgg
    11341 gctcggaaga ggcgggactg cgcggggcga aagtgtttgc ccggaaacat aaggaggact
    11401 tttccgatgt gcagacgctt tttattggtc tggagacgct ccgcgacccc gagtatctca
    11461 ccatctatga ccgggatatg aacgggatgg tgcgccatga tgaacaggtg tgcagtcttt
    11521 taaaagaggc gggtaaaatc tgcgggcttg atcttccttt cggcagcctc tttctcggct
    11581 cgtcggatgc cgccgccttc acgcaggcgg gctggcaggc ggggctactt gccgccatgg
    11641 atcctgcgcc gcccgcttac tatcacacac ggctggacaa ttgggacaac atgagccggg
    11701 actgcctgaa gacggctata caggtgacct gtgccgcctt gaagctgttt gatgaggagg
    11761 ctgaagagcc gatggaattt tcggataact aaaggaaaag aaagatcccc acagagcgtg
    11821 attcctggat taccatttcc atactgacta aatcttccga atccatgctg gcactctccg
    11881 ttctgttgac gaacggtatc cccgcccgta ccaaaaagaa tcccattgcg gcgttgattc
    11941 ctattctgac tctctttaca cgaaaagccg aaattcaagt gcaggcgccg gagtcctgtc
    12001 ccggcaaagg cagaggtgtt ttaaaaatac tcaaccttcc aaccacgcct gaagaaccct
    12061 gatccctttt tcagcgatag cctgttcctg cctggcatca gcggcaccgt caggatcgct
    12121 ttccggcggg agagcgtcat aaggggcttg gctcttgcgc cggaatgcac ttcgggttcc
    12181 ggacttagcc ggggtctttg aaggattcat aaatcacccc tgatcatacg gcgcatgctc
    12241 ctgctcttca agcgctcggg gtgctcttca gcattgccct accctctgga agaacactac
    12301 tatgctatca tgcattactc agaaaacgcg gggacatttc taaagagtta cgatcacatt
    12361 attattatat gccttgcttt tttaggcgac atgtgttagt atttttttgt aataaccatg
    12421 ggttacatca ctaaggaggt aagtgccatg gcattctcca atttgtctct cgccaatgcc
    12481 aaaaaaaggc attttgattt cagttgcttt tttgttcttg tgtgggggaa tggcttgagg
    12541 aaataatctg cctccggcac cactggagct tttaaattct gtcagcttcc aacagtgggt
    12601 tgcggacggg gacgaagtag ccaaagatat cttcttgccg gctggtacag aactcgtgtt
    12661 tatttccgtc acaggattac ctagagcctg gttcaatctc ggttatgttt cccctgcacc
    12721 ttctaccctt gtggcggaga cagacggtat ggcggctctg gatctcaccc attatctgta
    12781 ttaccatcgt gtctttcccg gccatgacga ccaggagatc gaggtgcttt tgggaatcat
    12841 tttcggcgat catgaaagct ttgagatcac ggtaacagta gtttgtgtac gcggtgcgga
    12901 catgagcaat tttcccggag atggttctct ctttacaggt accgtttacg aaataaacga
    12961 cgggagcacc gaagaagaag tgtctataaa tccgcctgaa atcttaaagc gcggagatat
    13021 gcctttattc attgccggag cacagggcac actgggtgat gtcctcgacg gcgacacggt
    13081 ttctgcctct tctgtcaagg gtacgttcct ttcttctgcg gtgggatatg cccgtgttgg
    13141 cgcaagaaca accagtatcg aactgggctg gcaagccaat gtcacgcaat acagtgcttt
    13201 ctgcatccct cttcattcac gatcattcac ggcagtacag cttaatatca tgcctgtctc
    13261 cgccgtgcag gccggagcca tgtggagcgc agactggagc gaattcggcg agacccggtg
    13321 gcaggaaacg ggcacgctgg tggctcagca gcccctggga aaatggcctt tcaaattttt
    13381 cgccgtcccc gaacacagcg tgccggaatc catcattgat attcagcccg gtgaccccac
    13441 agttctttat gcagcctatc acaaacgggg tacccccgaa gtgctcaaaa cagagtatta
    13501 cgcaggcaat gctgtgtctg atactggcta cgaagtcgcc tttgacggca ctgtcccctg
    13561 gaatgctgaa cttgcacttc ttctgatctc cacccgaagt gaagaaaaaa ttgctgtaaa
    13621 ttattcttct ccatcaggac taagcatgta ccggaagtct gagtattcgc ttccctctct
    13681 ggtcaagtcg ttcggtccct ccgaagaagc aggcatctcc acttcgcttt actgcatcaa
    13741 acggccgcag cttaatccgg gcggcattta tgatgagatg ataatacttg caccggaaga
    13801 aggcaatgaa gcggttcctg atacgattac acttctgttt ttacggggag tggatctgga
    13861 tgccttcagt aatccctggg aagggacttc acttttcccg caagtcactt atgaatccgg
    13921 aaccgccacc actattgccc gggaagtgaa ttcctggagt ttgccgcccg gaggtgccat
    13981 ggtcctttcg atactgaacg gcacggcctc cgttggcggg ctgacagata gcttgcaaca
    14041 gctcctttgg gcaggcggga cgccggacaa cagtctgacc acatcgggct cagccttgct
    14101 tatgctgccg ggtgacaata acgcggagct ccaatggcag gcaagtaccg gcgaggaagg
    14161 ctgggatctg cttcagatta tactgcctgc cactggtacg ggttccctga aagtaagctg
    14221 cagcgatgcc gaccaggccc cagaaaatcc aacctggaga attgataatg agccccgtcg
    14281 gtcaagcggt tatcaggata atggaatttc cgccggatgg cactggctgg aattttctga
    14341 cccggacccc tataccttgc agacgccgcc gggacgatgg atctatattg ccgctggcac
    14401 ctcgttgagt gagagcggcc gctatgccct ttcgggatat cctcatcccg tatatcagtt
    14461 ctctttcacc gccctcacca acgacactgg taatcttagt ctgaatcata ctctgattcc
    14521 ggaacttacc ggggatggcg gattctgttt ccggagactt gacaactgta attcttgccg
    14581 gctgcagcgg ggatactgtg gaggttgaac aaggggccgg caagacattc accccctggg
    14641 cgcgcggcgg aggactgggc ggaagcgccc ccggagagat ttccgtattc ttccgaaaaa
    14701 atcacagtat taaccgggaa agcctatggg atttcaagac cggacttccg aacagggctg
    14761 tatcaggaac ggtaactttt tttactaacg tggatgccgc tgctgtccga gcaggcctga
    14821 atgcagcagg tggaggcggt tcaaataagc ccgggcaaac cgtgaattta cttaacccgc
    14881 tcacccatcc ttccccatac gacatgctct atggtgccat acaatcacag aacataggtc
    14941 agcccagacc taatctgaac atttctaaaa aacttaccct gctgtccaat actccagcat
    15001 accgcgacaa agacagcata atcggtcaga tgctcgggta tacctgcctg cccacaccag
    15061 agaaacccga agctgagcag ggtcccgctt tcattgtgga acgcgactgg ggctcggtgg
    15121 aggccttgct gcctcatgct tccggcaatg taacagttac tattacacca ccggaactgg
    15181 cagcccgtcc ggaaatgaaa tggtctattc acggcggtga cggagacaag tcatccggtg
    15241 aaacaagttc cgcactgtgc ggtcaccaga aaattaaggt accgcaacct ctggattaca
    15301 agctgctcaa taaggaagat ttgattgtag aggtggcgcc cggcggctcc cttcaggtaa
    15361 cgctcaatta ccagatactt ccgcctacat ctttttccat catcattgct ccgaccaagg
    15421 ctgcagacaa tggggccgcc tggcggatca aagatcataa tccgcccgat tctttaaggc
    15481 atgacagcgg ttataaatat aacgatatta cggcagaaga tgtggaacgc ggctatgtga
    15541 ctgtcgagtt cagagaagca aaccacccga ccgatgggaa atatcccgca cccgaagatc
    15601 ggacctttgc aatcactgca ggaagcacca atacttcgat agtatcttat tatcaatatg
    15661 gttatgttcg cttccgtgtg gaaccgccgg aagcggcggc aatgggaccc ctatggcgta
    15721 tacggggaga tgacgagggt gacggctggc gcacctattc tcctgacgaa atcatcaggc
    15781 tgccgatcct gcggcggggc attgagtttt ctgatctgga aggctggcaa actcctgacc
    15841 ttgatatcca tgtccagtat agcgagattg atgatgctca aagttatgtc gttcagtatc
    15901 agccatatgg attgccccgt gtgcgcggca gatcctcctg gaacggcacg ccgggaggtg
    15961 ccactgtaaa cgtaaaaacg tccataccaa aggcgccttc tcctcaaatt tgcctgctct
    16021 ttctcacctg gcaaagcagt gccaacacga acagcgagcc cactcaagtt cagttgcaca
    16081 cgaccaattt taaccgcctg gtgatatctc ccgaaggaaa agtgcgcact gccatttatt
    16141 tcatagaagg aggtatgggt tacacttcac ccgcaggaga cgacctgcaa ataatctttc
    16201 cttccagcgc tgttccggat gcgcttcagg tcgaatgtat ttttctcgat gatgttgacg
    16261 gtggctcccc ctggtactct gcaagcagat caggggcgtc tcccgctgtg ccagaaagtt
    16321 accttgaata tccttggctg ccggcgacaa tgtttagctc attgagcact atcgcagatt
    16381 ctttttcccc ggattttgat tccgcttccg atatcagtga tcctgcactg attgggtatg
    16441 caacattcga tggtgcttcc ggcggtacgg cgctgatcag tggcgaaatg actggaaccc
    16501 gcccaagaat ctcctggaac tgcgagggcg actgggctct tgcatctatt gcgatacctg
    16561 cagcacaggg tgatgtccaa gtgaatattc acccggctgc ggcagtgacc gacggtgtca
    16621 aatggaatat taagcccaaa tatctatccg gcacaccgag cggagggacc cggaaagccg
    16681 ctttaggttt acagacgatt cttccgaaag atcacaacgg ctggaggctg gacccggcat
    16741 acgacaatac agttgaagta caacgtaccg ggacagctgt cggcgaactg caccttcttc
    16801 ctgcaccccg cgcaggtata cgcgtgaata tttttccggt cgaggctgtt gccgccggtg
    16861 ccaaatggcg cgtgtcggga atgggaccgg atgaatggca ggaaagcggc accgtctgga
    16921 caggattgaa agatctggac agatattatc tccccgaatt aagcatgatt gacggctggg
    16981 atcggccctt agcctctgtg aatcccgtta ccgtttccag cggcgaaata acggaagttt
    17041 ccgtacttta cacgcgtcgt ggcgctttac gggttacgct tatgaaag
//
LOCUS       DAOPWB010000070        16638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3525_length_16638_cov_6.308750, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000070 DAOPWB010000000
VERSION     DAOPWB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 16638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16638
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3525_length_16638_cov_6.308750"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(677..1150)
                     /locus_tag="PKN92_07850"
     CDS             complement(677..1150)
                     /locus_tag="PKN92_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48744.1"
                     /translation="MSLEALQEICLDYLSQSENPLVPLPHLYAHCIETTQGTCAVTPE
                     ELLSFLRKHNDVLVLENPAGQLAINEDQFLESGILMGTRAILKTRMPSEKELKDVFKL
                     QLLEMQQQLERALSHATEQGDSDRAAEIQKKINQSQTLLKRFAALCACRNEDLRL"
     gene            complement(1184..3649)
                     /locus_tag="PKN92_07855"
     CDS             complement(1184..3649)
                     /locus_tag="PKN92_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ48745.1"
                     /translation="MASEKLTADFTLHMIGHGHIDPVWLWRWTEGYSEVRTTFASALQ
                     RMQETSEFVFSASSACFYQWVKESDPALFEQIRERVAEGRWEIAGGFWVEPDCNIPCG
                     ESFVRHGLYSQRFFQREFGKRVKVGFNPDSFGHAGNLPQILKKLGMDCYCYMRPQPEM
                     GEKDYPDGTLFLWQSKDGSSVVTAQLPFCYTGQVEDIKKRAALLPASPRLHPQQKHAL
                     CFYGVGDHGGGPTREAVQFIHDWEKETLLPGVHLQFSSLEDFFNAVKSEIPESAMPVL
                     QEELQHHARGCYSVHSEIKRLNRKVEHALMTAERFATAAWLLGALDYPQDAFEKCWKD
                     LLFNQFHDLLGGTSLQSAYEDARDQLGAARHRADVILNQSVQSIARDVDTSAEGNPFL
                     AINPLPWPVWAAITLPPAVRTNLTDEFHVVNEFDDAVPIQAVRGERIGHTNHRFVAHL
                     PALGYQVFHARSGAKKYKHTLPLESGNDFLENYWWRIEFCERTGEIKRLFDKLHRIEV
                     LERGGILSALVDSGDTWGHSIDAFRIEAGRFGQAELKIVEAGDVFVTMRCRAFYDCSE
                     AITEYTLCRTTDHITVNMRVNWQQKHQALKFGFITNVQEGRAVWEAPYGYQERQADGM
                     EEPGGQWMDLTGTCEGRTYGFALLNDCKYGSDILKNTMRITLLRSPLYAHHDPERSQP
                     EELWQQMDQGWHTMRFALMPHTENWRDARIVKRAWELNAPAFVHMEARHPGKFKKQAG
                     LLGTESDHVLLTVIKCSEDGEEVVIRGFETAGKAAQTRLHLPYFDKAFCLSFEPHEIK
                     TIRINKDNWTYQQVNLLEEKTDS"
     gene            3912..5030
                     /locus_tag="PKN92_07860"
     CDS             3912..5030
                     /locus_tag="PKN92_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HNZ48746.1"
                     /translation="MAEKDLKAMYRTRTEGSFPEVLELLGRSYTKVEDLRYGTNPHQP
                     AAFYRPSEGASLVLGAYKMLKTGKSGLSQTNLEDMQHALGMLKYMERPACAVMKHCNP
                     SGVAIEWDGSSLAEIYERARDADSQAAFGSVVAFNTEVDEDTAAEIMGTIVEGVVAPS
                     FSEGALSCFHDFDRFKKNKDIRVVQIPPLNQLPKYLGDAVNSWEIKIFDDGSLVVAQP
                     YLSRMKSIEDFVPAYNDHPKKGRIDIGRQATSRELNDLLFAWYVNLHVRSNGVVIVRN
                     GQTLAVGTGEQDRVGAVQQAIHKAQTKYKGSNSLEGAVMASDGFFPFRDAVDAATEAG
                     ITALAQPGGSVNDYDAIVACNESNAAMLFTLERCFSHH"
     gene            5183..6124
                     /locus_tag="PKN92_07865"
     CDS             5183..6124
                     /locus_tag="PKN92_07865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNZ48747.1"
                     /translation="MRKFFFLFPLCVILLLTGSVDTFALELLDASPALRIAKWLQGAP
                     VDVTAQDDRLYVIEFWATWCPPCRRSIPELNALHDKYSAQNVVIIGITQEEEETVQNF
                     LKEQEMKYTVALDQDSYCWKNYAESSGVNGIPHAFLVRNGKLWWEGSPFDNLEKVIRD
                     MVAGSYSAEEAQKNREEARISEEIAQLLELWMQEYLVYANFGRDRASADALGVRILEK
                     GTAFPEMMGILAWHIAENDSLRYRNLEFADILSEKACSLTEYKDPDILDSRAKVLFAQ
                     GKRKEAIELQKKAIDLADNEEVKEMLLEGLQEMQETD"
     gene            6197..8320
                     /locus_tag="PKN92_07870"
     CDS             6197..8320
                     /locus_tag="PKN92_07870"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HNZ48748.1"
                     /translation="MTVFMMLCCSFLQLPDVFADRIAILKDQVAQLESIQVEEGSVLA
                     GKSLLEEVRQLIEASPSTDESFDALYYRLDLCRQWLWNHAADRPRKPHNNFQEEEDCW
                     RVGNELLTLRLEKSNLAMTVNTAKQCWRFAPSRETDLKLQSGSFPLAAVSLKTAEAFQ
                     TGYGTGMTLLLKGYEAAPDLEIHITLLLIGSEIVMDLAMNEPETGFSALNWPPEVTLE
                     PEEKIWSVVPRMQGMLLPGNWSHALHQEELVHSRTLYMPWWGHVREGCGVQIILETTD
                     DVGAVIDHPAGGPTRIWTRWHASLGRFNYLRTVRMVFDDESDYVTMAKRYRKFVQERG
                     RWTSLAEKLLRTPNLRMVIGKPVIHVGALYHFVPQAQLFNKNRVENNHSLQTFDVIRE
                     KLEALSRKGVSAAYVHLDGWGYYGYDNAHPDPLPVGALQGGWDGLKRLADSCAAINYL
                     FAVHDQYRDFYKNAASFDERLAILRADGTKDECSVWCGGPQTILNARFAPDYVRKNHD
                     LFKEHGISVKGAYLDVFSVVPLEESARPAHPMSRAECARYRLECFELLRARGYVVSSE
                     EPVEYAVNSLDLVHHAPYPTYPNIGGGEACGIPVPLFNLVYHDALLVPWDFGEDGGWG
                     IPDGDAGRLYCLLHAGLPYLDPSAEAEVIERTLEAAAWSERCAFSEMQSHEFVSGDFR
                     KQRAVYANGASVFVDFDTKEYVLTP"
     gene            8417..11332
                     /locus_tag="PKN92_07875"
     CDS             8417..11332
                     /locus_tag="PKN92_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNZ48749.1"
                     /translation="MSGKKRSSSSVSVLQIILMVTLVLIAMIGIAFMLIPRKPVAPPV
                     SRVSYEERPESQKKNYSPVPSIESNEPAPITEPQKISEKKVIEEKPAEPEKELPFHLL
                     GTVVDQKTSKPIAGARVRVQRKTSAEDEVLFASFSGEDEKTLRQRAKEQQFSNSNRAG
                     EFKVPLEFPGQYEVEVTAVGYAKGTLLSPALGDSAAEVKISLALSTGASIAGRVTVAE
                     TGEGVGGLTVQADGFSGNYAITDKTGNYELRGLTPGLIGVTIDLRGTPYMSGKSLPYQ
                     KVAISSADQEVKNIDFTVEAAGVIWGYVLSPKQEPISGTNLVLASSQSVLSQALSQIA
                     KKTPPVTGQSGADGYYELTGVPFNEEWHVHASSDEFAPQLSDPIILTKTQRNVRVDIF
                     LFAGSNVYGTVVDSQGKPLPGAQVTCLPAYSTFFSNLKTPQAFRHTSSDANGQFMINE
                     LPGGEYQIFAQKKAYKVSPLGFPIHPDGYSDLTNVVLTLNSAEQGDHEIFGTVVDDRG
                     QGLDGVNLTLSGVTLGGLEGTQRTETTAGNGKFRFTGISAGQYTIIASKDGYSPTTVR
                     RARLNEETKILMKQSALVRGRVLIKENNQAPESYQVGAYPISESTGSPSLLGMMRDAA
                     TEEEFFAPDGSFSLAIHAGTYRLEASARGFTPARQEITLEAGQVVDNILLLLDEKGGR
                     IAGTVFAKDNANITGAQVTLLEASSPAEALMMLATANIPENRIYSVGEDGTFFFESLP
                     PGEYVAIAQHPNYPNAQSELIILTEGGREENVRIHFSSGGALEGYVYSEGRPVPNAVV
                     MVVGNGVTGHANADNTGYYYIGDLSAGLYQAMVTDISAGDLTTIYDARGVQVMVEEGK
                     ATRYDFGTQEGARIEGQCTPGPSNMLGGRAVLQRPGYAQIPLGETVDVTELLGQSVGI
                     NPAGQFVMEDVMPGEWQLDIFYFELGIVNPLEVRYVHTEFIQVEQGEVLPLTLDIMF"
     gene            11382..12302
                     /locus_tag="PKN92_07880"
     CDS             11382..12302
                     /locus_tag="PKN92_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48750.1"
                     /translation="MTAVTELIDVRVAYGDVPVLDGVTITFEEGATGLLGPNGAGKST
                     LIKTLLGFNRMSGGSLKIMGYAMPGEALQARRHLGYMPEKEISSPKISAVSFLTYCGR
                     ICGMSRVDSLERAHEVLNYVKMGDSRYRTMESYSTGMLQRVKLAQAILHDPRLLLLDE
                     PTNGLDPDGRIEILDLIKEIAKRPGISVILSSHLLPDVQHVCKQVVMINNGKVVKSGT
                     IQSLTTPQERVFELRFRDKADQCVAALTDAGCLCRRMFPADIWLTRCPESVRSADLFR
                     IVRRCNSQIRHFQPARNSLEDIFLEAVGRK"
     assembly_gap    12418..12517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12518..12949
                     /locus_tag="PKN92_07885"
     CDS             <12518..12949
                     /locus_tag="PKN92_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48751.1"
                     /translation="ALPGICLVILHNLLLPGMSTLAASWWWPLAILAYSTALVLPAVF
                     SILAASALMPSQNYAAIIVFMVLIANTSFAGILSGLLRMRNLLALSFPMSVRRLGELL
                     FDEKRILFTTSWQWCLGYVAVVCAIALLIIVIKIRRQEVMS"
     gene            12946..13863
                     /locus_tag="PKN92_07890"
     CDS             12946..13863
                     /locus_tag="PKN92_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48752.1"
                     /translation="MSPIIEACGLSKWFGEVVALNNLDIALDSPVTGLLGPNGAGKST
                     FIKLALGLYSPSKGSIRIFGEAPRNNHRVMRRIGYCPETDYFFENSTGFEFVYWLNRY
                     WGMQHRQAVTSAEAACDLVNMTERMHDAIDTYSKGMRQRIKIAQALTGCPELLFLDEP
                     MNGLDPKGREEMYLLIQKLAGKGMTVVVSSHILYEIERITDTVVLIYGGTLLAQGRVR
                     DIRDLIDKYPQAIRIRTTQARTLAERFITQEAVSSLEIEENSILVRTRDPNFTYEMLN
                     SIALEDAELIQGIACSDDNLQSVFDYLTR"
     gene            13879..14796
                     /locus_tag="PKN92_07895"
     CDS             13879..14796
                     /locus_tag="PKN92_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ48753.1"
                     /translation="MISFINDTQTAAGMNITKEDLFFRPDNRPDHSSYFQRFSASCAH
                     AMSMIARRKRLAVAALIAFLPVLVPLALAFFSTSQFAESGNNVFVRLAEDLHINVFTP
                     LLALFFAGMLIAEDIEMRTMIYILTRPMPRSAWVLGRFCAYIFVASLILISSAVLTFA
                     ASCVHSNLSLMSAGDIKLLLHYCGVMVLGLFAYGAFSVFLGAFTRRPIVFGVILFYGW
                     QHLATLIPGLVDFFTIKKYTTAMLPLLATQRHNPVIETVLGNIQREVFLVGMPKALAA
                     LVFITVVSLLLTIYATYRREYASDRAAGG"
     gene            14826..>16638
                     /locus_tag="PKN92_07900"
     CDS             14826..>16638
                     /locus_tag="PKN92_07900"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNZ48754.1"
                     /translation="MSGKWEPDTSQFYALSGEENSAADSIQTALADFEHYFNAFLKAL
                     DGQAELKDLLLDGTEEWRRLLSQKLLTQISEKACLIVVFAGGTNSGKSTLFNLTLQKK
                     LSEVRSTAAATKAPVLAVAPERLPDAINNKILVDFHARNQAFPGQSENPQEKEDLLYV
                     ASAENLPFSMALMDTPDVDSVIEKNWSIAEKIRAAGDVMIAVLTPQKYNDEKNVAFFR
                     IAHQSGRIIVPLMNRVEPSHTPDITKAQLNQFMGAIGFTAERAFTVPKDYSLESLPDL
                     PIMDLENQISLLDYLKRMDMGTVKIQVYKASLEYFLEQTASFLERCKNAEAQIRAFNA
                     ECLEEAEKAAAVFSPERRSNIKTGIRQCIQTSRSVIVQKMSFLLALLSPLYLPLKNFI
                     FKDDVQISQGKNKKQFQVDSAKENAARAQDAIRDSVLIYSNWFRKKLNEATKDTSFFL
                     LKEPRDNSANVEVIRKTLEKDAEGLVPEDTLLGTGLQEGWPIQSGKITALQWIDATFV
                     LAPYIFLLLLLSKGLSAIYPIICILPVFYMLLHVRRIIERSLEKQIRVFLSPAYHRQK
                     ERLKEELYRVVAKPIVGAVESALALFDEEHIKQLGSCR"
BASE COUNT         4169 a         3865 c         4224 g         4280 t
ORIGIN      
        1 tcatgaaaca acgaaactct cataacgact ggaccaaaga atgggggctt gacagggcca
       61 tggcggggcg gtagtggcgg aagagggcgc ggtattgttt cagggcggag tcgaattcgt
      121 ggagggcgaa gatgcggggc atgttgacaa aaccgttctg gccgtagata ttgccgtagg
      181 ggtcatattc gatggcgcgg gcgctgttct tggttgcaaa gcgccattgc cgcactgtgc
      241 ccaggtggtc gcccccctcc ccccggacga ttcgatgtga caaaaataga aaacatcttc
      301 aatatccttc cttggacaaa aatttactgt cgaaaaacaa gattaaaaaa atgatattct
      361 actttccaat aatcggtata gaccagcatg gcataaatgt caacacctca ctctgactta
      421 gggacaagac actttgccaa tcgagggcgt tattaggacg aacatgcagc atcaggatca
      481 taaccacgac tccgatagca atcggaacaa gatcgtgggt gcagccccaa aaggcgtcgt
      541 tcttacatcc ctgcgattca gaggtgcaaa gagagcaaac atctttcagc tccttttgta
      601 ctacagcgtg tatcacagaa acacagtcta aatgtatatt tctgctcttc tataagaacg
      661 gccggtattt aatcatttaa agcctaagat cttcgttgcg gcacgcgcac aacgcggcaa
      721 atctcttcag gagtgtttgg ctctggttga tttttttctg aatttccgct gcccgatcgg
      781 aatcgccttg ttcagttgcg tgtgacagtg cccgctccag ctgctgctgc atttcaagca
      841 gctgcagttt gaagacatct ttcaactctt tttcagaggg catccgtgtt tttaaaatag
      901 cgcgggttcc catgaggata cctgactcta aaaactgatc ctcattaatt gccagctgtc
      961 ctgccggatt ttcgagaaca agcacatcat tgtgttttct cagaaaagaa agcagctctt
     1021 cgggagtgac ggcgcaagtt ccttgagttg tttcaatgca gtgggcataa agatggggga
     1081 gaggaaccag tgggttctcc gactggctca aataatcgag acaaatttcc tgcagtgctt
     1141 ccagtgacat atttctttcc agagagtgcg ccttgaaagg agctcaagag tctgtttttt
     1201 cttcaagaag gtttacttgc tgatacgtcc agttgtcctt attaatccgt atggtcttga
     1261 tttcatgcgg ttcaaagctc aggcaaaatg ccttatcaaa atagggaaga tgcagccggg
     1321 tttgcgctgc tttgcccgct gtttcaaaac ctcgtataac aacttcttcg ccgtcctcgc
     1381 tgcattttat tacagtgagc aacacatgat ctgattcagt accgagcagc cccgcctgtt
     1441 ttttgaattt accggggtgc cttgcttcca tatgcacgaa tgcgggagca ttgagttccc
     1501 aggcacgttt tacgatgcgt gcgtcgcgcc aattttccgt atggggcatc aaggcaaaac
     1561 gcatggtatg ccagccctga tccatctgtt gccaaagttc ttccggttgg ctgcgctcgg
     1621 ggtcatggtg agcatataaa gggcttctca aaagagtaat gcgcattgtg tttttcagga
     1681 tgtcggagcc gtacttacag tcgttgagca gtgcaaagcc ataggtgcgt ccttcgcatg
     1741 tgccagtaag atccatccac tgaccgcccg gctcttccat accgtcggct tgtctttcct
     1801 ggtagccata gggtgcttcc cagacggcac gcccctcctg aacatttgtg ataaagccga
     1861 atttcaatgc ctggtgcttt tgttgccaat tgaccctcat gttcacagtg atgtgatctg
     1921 ttgtgcggca taaggtatat tctgtgattg cttcggaaca gtcgtaaaaa gcacggcagc
     1981 gcatcgtcac aaaaacatcc cctgcctcta ctattttcaa ttccgcctgt ccgaagcgtc
     2041 ccgcttcaat cctgaatgcg tctatgctgt gtccccaggt atcgccggaa tcaaccagag
     2101 cgcttaatat gccgccccgc tccagcactt caattctgtg gagcttgtcg aacagtcttt
     2161 ttatttctcc ggtgcgttca caaaattcaa tccgccacca gtagttttca agaaaatcgt
     2221 tcccgctctc caaaggaagg gtgtgcttgt atttttttgc gccactgcgg gcatggaaga
     2281 cctgatagcc taaggcaggc aaatgggcaa cgaaacggtg gtttgtgtgc ccgatccgtt
     2341 caccccggac ggcctgtata ggcacagcat catcgaattc attgacgaca tgaaactcat
     2401 ctgttaaatt ggtccgaacg gcagggggta acgtaatggc agcccacaca ggccagggca
     2461 gcgggttaat tgcaaggaag ggatttcctt ctgctgaggt gtccacatcg cgggcaatgg
     2521 actgaacaga ctggttcagg ataacatcgg ctctgtgtct cgctgcgccg agctggtctc
     2581 ttgcatcttc gtaggcactt tgcagactgg taccgcccag caggtcgtga aactggttga
     2641 agagcaggtc tttccagcat ttttcaaaag cgtcctgagg atagtccagc gcacctaaaa
     2701 gccatgcggc agtggcaaaa cgttcggctg tcatcagagc atgctcaact tttctgttga
     2761 gtcgttttat ttctgaatga acactgtaac atcctcgggc gtgatgctgc agctcttcct
     2821 ggagcaccgg catggctgat tcaggaattt ctgatttaac ggcgttgaag aaatcttcca
     2881 aagatgaaaa ttgaagatgc acgccgggga ggagcgtttc tttttcccaa tcgtgaatga
     2941 attgcaccgc ttctctggtc gggcctccgc catgatcacc tacaccgtaa aagcataatg
     3001 cgtgtttctg ctgcggatga agccgcggac tggcgggaag gagagcggct cgtttcttaa
     3061 tgtcttccac ttgcccggtg taacagaagg gcagttgtgc cgtgactaca gaagagccgt
     3121 ctttactctg ccataagaag agggtgccgt cgggatagtc cttttcgccc atttcaggct
     3181 gcgggcgcat atagcagtag cagtccatcc ctagtttttt gaggatctgc ggaaggttgc
     3241 cggcgtggcc gaagctgtcg ggattgaagc ccactttaac ccgtttaccg aattcccgtt
     3301 gaaaaaagcg ctgtgaataa aggccgtgtc ttacaaagga ctcgccacag ggaatattgc
     3361 agtccggttc gacccagaag ccgccggcaa tttcccagcg tccttcagcc acccgctctc
     3421 tgatctgttc gaagagcgcc ggatcacttt cttttaccca ttgataaaaa caggcgctgc
     3481 tcgctgaaaa aacaaactct gaagtttcct gcatgcgttg gagcgcactt gcaaaagtgg
     3541 tgcggacttc actgtaccct tctgtccagc gccataacca tacaggatcg atatgcccgt
     3601 gcccgatcat gtggagtgta aaatcagcag taagtttttc acttgccata gcgctgcgct
     3661 ccctgatgtt aagatgacga ccgcacctta tatatatgtt catattatgg cctaaggcga
     3721 ctgcctgctt ccagtgtaaa gatgaagaaa aggacgcgtc ataatttctt aagaacggtc
     3781 ttgcagttat tttgtcttcc gcacatggct cgtataaaat aaggatgttc aggctgtcgc
     3841 agttactgtt tcaggtgcaa cagctttttt tgttaactga aagatttact ctgaaagaag
     3901 gaaacacagg aatggctgaa aaagatctga aagcaatgta tcgaacccgc acagaaggca
     3961 gtttccccga ggtactggaa ctgctgggtc gctcctatac gaaagtcgag gatctgagat
     4021 acggaaccaa tccgcatcag cctgccgctt tttatcgccc ctctgaaggt gcctcgctgg
     4081 tacttggtgc ctacaagatg cttaaaaccg gaaaaagtgg tttgtctcag acgaatcttg
     4141 aagatatgca acatgctttg ggcatgctga aatatatgga acgccctgcc tgtgccgtca
     4201 tgaaacactg caatccctcc ggtgtggcga ttgagtggga cgggagcagt cttgccgaaa
     4261 tctatgagcg tgcccgtgat gcagacagcc aggccgcttt cggcagcgtg gtcgccttca
     4321 atacagaagt ggatgaagat acagcagcag aaatcatggg caccattgtg gaaggcgttg
     4381 ttgcgccttc gttcagcgaa ggggcgctta gttgctttca tgactttgat cggttcaaga
     4441 aaaataaaga catccgtgtt gttcagattc cccctctgaa tcaactgcca aaatatctgg
     4501 gtgatgctgt caattcctgg gaaattaaaa tatttgatga cgggtcactg gtcgtggcgc
     4561 aaccgtatct ttcccggatg aagtctattg aggattttgt tcccgcatac aacgatcatc
     4621 cgaagaaggg ccgtatcgac attggcaggc aggcaacctc aagagaactg aatgacttgc
     4681 tttttgcctg gtatgtaaac ctgcatgtcc gttccaatgg cgttgtgatc gtgcgcaacg
     4741 gccagaccct tgcggtggga acaggtgagc aggatcgggt gggcgcagtg cagcaggcca
     4801 ttcacaaggc tcagacaaaa tacaaaggca gtaactcgct tgaaggcgca gtcatggctt
     4861 cagacggatt cttccccttc cgtgatgccg ttgatgccgc caccgaggca ggaatcacag
     4921 cgttggcgca gcccggcgga agcgttaatg attatgatgc tattgttgcc tgcaatgaaa
     4981 gcaatgccgc catgttgttt acgcttgagc ggtgtttctc tcatcattaa aagagcgctt
     5041 acccttggca tagtgtgttc gttgtcttgc ggacgtttcg gttgcgttgg aatgtgcggc
     5101 aaaggcagtt tgtttcagcc ataagtgccc ggtaaggcta tgtatgtaag tatcctcttg
     5161 aaccgctgcg aaagggtgtg tgatgcgcaa attctttttt ctgttcccgc tttgtgtaat
     5221 actgcttctg acaggctctg ttgatacgtt tgcattagaa ctgcttgatg cgtctcctgc
     5281 cttacgcatt gcaaaatggt tacagggtgc acctgtggat gtaacggcac aggacgaccg
     5341 gctttatgtc atcgaattct gggctacctg gtgtccgccc tgccggcgca gtatccctga
     5401 attgaacgca ttgcatgata aatacagtgc acagaatgtc gtgatcatcg gaattactca
     5461 ggaagaggaa gaaacggtac agaattttct caaagaacag gaaatgaaat ataccgttgc
     5521 gctggatcag gactcatatt gctggaaaaa ttatgctgaa tcttccggcg taaacgggat
     5581 tcctcatgct tttctcgtac gtaacggcaa actctggtgg gaaggcagcc cctttgacaa
     5641 tcttgaaaaa gtaatccgcg acatggttgc agggtcttat tctgcggaag aagcacaaaa
     5701 aaacagagaa gaagcgcgca tcagtgaaga gattgcgcag cttcttgaac tttggatgca
     5761 ggaatatctg gtctatgcta atttcggaag agacagggca agtgccgatg ctttaggtgt
     5821 tcgtatcctg gagaaaggca cggcgttccc ggagatgatg ggtattcttg cctggcatat
     5881 tgctgaaaat gattctttgc gctacagaaa tctggagttt gcagatattc tttcagaaaa
     5941 ggcatgcagc ctcaccgaat ataaggatcc tgatatcctg gacagccgtg caaaggttct
     6001 ttttgcacaa ggaaaaagaa aagaggcgat tgaactgcaa aagaaagcaa tcgatcttgc
     6061 agacaatgaa gaagtgaaag agatgttgct cgaaggtctg caggagatgc aggaaacgga
     6121 ctgagacagg cggttttgac aggtcgcacc aggctgtcag gaagtacaat gttaaaaact
     6181 aaaggagatc tgtatagtga ctgttttcat gatgctttgc tgtagtttcc ttcagcttcc
     6241 tgacgtattt gccgaccgaa ttgcaatcct caaagatcag gttgcgcagc tggaatccat
     6301 tcaagttgaa gagggctccg ttcttgcggg aaagtctctg ctcgaggaag tgaggcaatt
     6361 aatcgaagcc agtccttcaa ccgatgaatc atttgatgca ctttattacc gcttggatct
     6421 ttgcaggcaa tggctgtgga atcacgctgc tgacagaccc cgaaagcccc acaataattt
     6481 tcaggaagaa gaagactgct ggcgcgtcgg aaatgagtta ctcaccctcc gcctggaaaa
     6541 gagcaacctt gccatgactg ttaatacagc aaaacagtgc tggcgttttg ctccttcccg
     6601 tgaaactgat ctgaagcttc aatcgggatc atttcctttg gctgccgttt ctcttaaaac
     6661 agccgaagcc tttcagacag gctacggaac cggtatgacg cttctattga aaggatatga
     6721 ggcggctccg gacctggaaa ttcatattac attgctgctg atcggcagcg agatcgtgat
     6781 ggatcttgcg atgaatgagc cggagactgg cttttccgct cttaattggc ctcctgaagt
     6841 gactcttgaa cctgaagaga agatctggtc tgtggttccg cgtatgcagg gaatgctgct
     6901 tccgggtaac tggagtcatg cgctccatca ggaggaactc gtccattcac gaaccttgta
     6961 catgccctgg tggggtcatg tgcgtgaggg atgcggcgta cagattatcc ttgaaacgac
     7021 cgatgatgtt ggtgctgtga ttgatcatcc tgcgggcggt cctacgcgga tatggacgcg
     7081 ctggcatgct tctttaggca ggtttaatta tctccgaacc gtgcggatgg tttttgatga
     7141 tgagtccgat tatgtgacca tggcgaagcg gtaccggaag tttgtgcagg agcgggggag
     7201 atggaccagc cttgctgaga aactgctgag aacaccaaac ctgcgcatgg ttatcggcaa
     7261 gccggtgatc cacgtcggcg ctttatatca ttttgtgccg caggcgcaac ttttcaacaa
     7321 aaaccgtgtg gaaaataatc attcccttca gactttcgat gttatcagag aaaagttgga
     7381 ggccctttcc cgtaaaggag tatcggcagc ctatgtgcat ctggacggtt gggggtatta
     7441 cggatatgac aatgcccacc ctgacccgtt gcctgtcggt gctctgcagg gaggctggga
     7501 cggtctaaaa cggctagcgg attcctgcgc tgcgataaac tatctctttg ctgttcatga
     7561 tcaatacagg gatttttata aaaacgcagc ttcttttgat gaacgcctgg caattctgcg
     7621 ggcggatggt acaaaagacg aatgcagcgt ctggtgcggg ggcccgcaga ctattctcaa
     7681 tgcacgtttt gcgccggact atgtgcggaa aaaccatgat ctttttaaag aacatggtat
     7741 ttcagtaaaa ggtgcctatc tggatgtttt ctccgtggtg cctttggaag aaagcgcccg
     7801 gccggcccat cccatgtcac gtgccgaatg cgcccgctat cgtcttgaat gttttgaact
     7861 gcttcgtgcc agaggctatg tggtcagctc tgaagagcct gttgagtacg cggtgaattc
     7921 tctggatctg gtgcatcacg caccctaccc cacctatccc aatatagggg gtggggaagc
     7981 ttgcggtatt ccggtaccgc tgtttaatct tgtgtatcat gatgcgctgc ttgtgccctg
     8041 ggattttggc gaagacggcg ggtggggcat acccgacggc gacgccggca gactctattg
     8101 tctgctccac gcgggtttac cttacctgga tccgtcggcg gaggcagagg tgatagaaag
     8161 gacactggaa gccgcagcat ggtcggaaag atgcgctttt tcagagatgc aatcacatga
     8221 atttgtcagc ggtgatttca gaaaacaaag ggctgtgtat gcgaacggtg cttcggtgtt
     8281 tgttgatttc gacactaagg aatatgtact tacgccatga cgactttcac agcaaactgc
     8341 gcgctgtata cttataattg cgctttttcg ttatagagtc gaattcctcc tcattattaa
     8401 gtgcaaggga agctatatgt ccggaaagaa acgttcctct tcatctgtat cggttttaca
     8461 gatcattctt atggtgacgc tggtactgat agcaatgatc ggcatcgctt ttatgctgat
     8521 cccgcgcaag cctgttgcac cgcctgtttc cagagtcagt tatgaggaac gtccggaaag
     8581 tcagaagaaa aactattcac ctgttccgtc aatagaaagt aatgagcctg ccccaattac
     8641 tgaaccccag aaaatttcag aaaaaaaagt catcgaagaa aagcctgcgg aacctgaaaa
     8701 agagctgcct tttcatctcc tcggtacagt ggtagaccaa aaaacatcga agcccattgc
     8761 aggcgcccgt gtgcgcgtac agcgaaaaac gtcagcagaa gatgaggtgc ttttcgcgtc
     8821 tttttcagga gaagatgaaa aaacacttcg acagcgggca aaagaacaac agttttcgaa
     8881 ttcaaaccgc gcaggcgaat ttaaagtgcc tttggaattt cccggacagt acgaagtgga
     8941 agtcactgct gtaggatatg caaaaggaac actattgtct ccagcgttgg gtgactctgc
     9001 tgcagaagtg aaaatcagtc tcgctctttc taccggggcc agcattgcag gtcgtgttac
     9061 agtggctgag acaggagaag gtgttggcgg ccttaccgta caggcagacg gtttttccgg
     9121 aaactacgct ataaccgaca aaacgggcaa ttacgagctg cgtgggctga cgcctggact
     9181 gataggggtc accattgacc tccgtggcac gccttatatg agcggaaaaa gccttcctta
     9241 ccagaaagta gccataagca gtgctgatca ggaagtaaaa aatattgatt tcaccgtgga
     9301 ggctgccggt gttatctggg gatatgtgct ttctccgaaa caggagccca tcagcggaac
     9361 gaatctggtg ctcgcctcct ctcaaagtgt cctttcacag gccttaagcc aaatcgcaaa
     9421 gaaaacccca ccggtcacag gtcaaagcgg agcagacggt tattatgagt tgacaggcgt
     9481 tcccttcaac gaagaatggc atgtacatgc cagcagcgat gagtttgccc cgcagctgag
     9541 cgatccgatt attctgacta aaacgcagcg taatgtgcgg gtggatatat tcctttttgc
     9601 aggaagcaat gtgtatggaa ccgtcgtcga cagccagggg aaaccgctcc ctggtgcaca
     9661 ggtaacttgt cttcccgcct attccacttt cttcagcaat cttaaaactc cgcaggcgtt
     9721 caggcacaca agtagtgacg ctaacggcca gtttatgatc aatgaacttc cggggggcga
     9781 ataccagatc ttcgcacaga aaaaggcgta caaggtcagt cctctcggtt ttcctattca
     9841 tccggacggg tacagcgacc tcacgaatgt ggtgctgaca ctcaatagtg cagaacaggg
     9901 ggatcatgag attttcggta ctgttgtcga tgacagagga cagggactgg atggcgtcaa
     9961 tctgacgctt tccggagtta ccttgggcgg gctggaagga acgcagcgga cagaaaccac
    10021 agccggtaac ggcaagttcc gttttaccgg catcagcgca gggcaatata ccatcattgc
    10081 ttccaaggac ggttattctc caacgacagt caggcgggca cggctcaatg aagagaccaa
    10141 aattttgatg aaacagtccg ccctggtaag aggaagagtc ctgattaagg aaaataacca
    10201 ggcaccggaa agttatcagg ttggtgctta cccaatttcc gagagtaccg gttcgccaag
    10261 tcttctgggc atgatgcgcg atgcggcaac ggaagaagag ttttttgcgc ctgatggaag
    10321 tttttcattg gctattcacg caggaaccta ccgccttgag gcatcagcac gaggctttac
    10381 acctgcacgg caggaaataa cgctcgaagc ggggcaggtg gtggataaca ttctcctttt
    10441 gctggatgaa aaaggcggtc gtatcgccgg aactgttttc gcaaaagaca acgccaatat
    10501 taccggtgca caggtcaccc tgttggaagc atcatcgccg gcagaagcat tgatgatgct
    10561 tgccaccgcc aatatacccg aaaacagaat ttacagcgtc ggagaagacg gcactttctt
    10621 ctttgaaagc ctgccacccg gagaatatgt tgctattgca cagcacccta attatccgaa
    10681 tgcccaaagt gagctcatta ttttaaccga gggcggacgt gaagaaaacg tgcgtataca
    10741 tttcagttcc ggcggtgcgc tggagggcta tgtatacagc gaggggcggc ctgtgcccaa
    10801 tgctgtcgtg atggtcgtgg gcaacggcgt aacaggtcat gccaatgcgg ataatacagg
    10861 gtattattat attggcgatc tttccgccgg gctgtatcag gcaatggtaa ccgatatcag
    10921 tgcaggcgac ctcactacca tttatgatgc ccgtggtgtg caggtcatgg tggaagaagg
    10981 taaagcaacc cgctatgatt tcggtactca ggagggcgcc cgaatagaag gacaatgcac
    11041 gcccggtccg tccaacatgc tgggcggtcg tgctgtgctg cagcgccccg gctatgcgca
    11101 gatcccgttg ggtgaaacgg tagatgtcac ggaactgctc ggtcagagcg tgggcatcaa
    11161 tccggcagga caattcgtga tggaagacgt tatgccgggc gagtggcagc tcgatatttt
    11221 ctattttgaa cttggtattg ttaatcctct tgaagtacgc tatgttcaca ctgagtttat
    11281 tcaggttgaa cagggcgaag tccttccctt aacgctggac atcatgtttt gacagcctta
    11341 gtgatgccaa aacctaacca gacaaaattg gatgagaact tttgactgca gtaacagaac
    11401 tcatagatgt gcgtgttgct tatggcgatg tgcccgtttt ggatggagtc accatcacgt
    11461 ttgaagaagg cgcaacaggg ctgctcggtc ccaatggagc agggaaaagc acgctgataa
    11521 aaacgctcct cggttttaat cgaatgagcg ggggcagcct gaaaattatg ggatatgcaa
    11581 tgcctggaga ggcgctgcag gcacgtcgac atctcggata tatgcccgaa aaagaaatat
    11641 ccagccccaa aattagtgct gtttcttttc tgacttattg cgggcgtatt tgcgggatga
    11701 gccgtgtcga ttctcttgaa cgtgctcatg aagtcttgaa ttatgtcaag atgggcgaca
    11761 gccgttacag gacaatggaa agctactcaa cagggatgct gcagcgggtt aaacttgccc
    11821 aggcaatcct ccatgacccg cgcttgctcc ttcttgatga gccaacgaac ggacttgatc
    11881 cggacggacg gatagaaatt cttgatctca tcaaagaaat tgcgaagcgt cccggtattt
    11941 ctgtgatact ctcttcccac ctcctgcctg atgtgcaaca tgtctgtaaa caggtggtca
    12001 tgatcaataa cggcaaagtc gttaaatcag gaacgattca atctctgacg accccgcagg
    12061 aaagggtttt tgagcttcgt ttcagagaca aagcagacca gtgcgttgct gctctcaccg
    12121 atgccggctg cttgtgccgc cgtatgtttc cggctgatat atggcttaca cgttgccccg
    12181 agtccgtgcg cagtgcagat ctctttcgca tcgtgcgcag gtgcaacagt cagatcagac
    12241 attttcaacc tgcccgaaac agtctggaag atatatttct tgaagcggtg ggaaggaaat
    12301 aaagttgcct atttttaccc aaagttacag aagttttgaa ggaaagcccc agcggcattt
    12361 ccgatggctc attgttgttg aacaggaatt taaaaccctt acaaaattta aaatattnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca ctcccgggaa tttgcctggt
    12541 catcctgcat aatctgctgc tcccgggcat gtccacgctg gctgccagct ggtggtggcc
    12601 tttggcgatt ttggcatatt ctaccgcact tgtgctgccc gccgttttct ctatcctggc
    12661 agcatcggca ttgatgcctt cgcagaatta tgccgccatc attgttttta tggtgcttat
    12721 agccaatacg agctttgcag gaattctttc cgggcttctc cggatgcgta acctcctcgc
    12781 tctttctttt cccatgtctg taagacgctt aggcgaactc ctttttgacg aaaaaagaat
    12841 tctatttact acgtcctggc agtggtgtct cggatatgta gctgttgtgt gtgccatagc
    12901 gcttctgatc atcgtaatta aaattcgacg gcaggaggtt atgtcatgag cccgattatc
    12961 gaagcatgcg gtttgtcaaa atggttcgga gaagtagttg ctctgaataa tctcgacatc
    13021 gccctggact ctcctgttac agggctgctg ggtcccaacg gtgccggcaa aagcactttt
    13081 atcaaactgg ctctggggct ttattcgcca agtaaaggct ccatcaggat tttcggagaa
    13141 gcaccaagaa ataatcatcg ggtcatgcgc cgtataggct actgtccgga aactgattat
    13201 ttttttgaga attctaccgg ctttgaattt gtttactggt tgaatcgtta ttggggaatg
    13261 cagcacagac aggccgttac ttcagccgaa gccgcctgtg atctagtcaa catgacagaa
    13321 cgcatgcatg acgctattga tacctatagc aaaggaatgc gccagcgcat aaagatagca
    13381 caggctctga ccggatgccc tgaactgctc tttttggatg aaccgatgaa cgggcttgat
    13441 ccaaaaggaa gagaagagat gtacctcctt attcagaaac tggcggggaa aggcatgaca
    13501 gtggttgttt ccagccatat tctgtatgag atagaaagaa taacagatac agtggtgctt
    13561 atttacgggg gaaccttgct ggcgcagggg cgcgttaggg atattcgtga cttgatagac
    13621 aaatacccgc aagccatacg gatcagaacc acacaagcgc gcacccttgc tgaacgcttt
    13681 attactcagg aagctgtcag ctctctggaa attgaagaga attccattct ggtaagaacg
    13741 cgagacccta acttcaccta cgaaatgctg aattccattg ccctcgaaga tgcagaactg
    13801 atacaaggga tagcgtgttc cgacgataat ctgcaatcag tctttgatta tttaacccga
    13861 tagcgttccg gtatttttat gatatctttt atcaacgaca ctcagacagc ggctggtatg
    13921 aatatcacta aagaagatct ctttttcagg cccgacaacc gcccggatca cagcagctat
    13981 ttccagagat tttcagcatc gtgtgcccat gccatgagca tgattgcgcg gcgcaaacgt
    14041 ctggctgtcg ctgcattaat cgcttttctt ccggtgctgg tcccgttggc acttgctttt
    14101 ttttccactt cgcagtttgc tgaatcaggg aataatgtat ttgtaaggct tgccgaggac
    14161 ttgcacataa acgtctttac accgctgctg gcgctttttt ttgcgggcat gctgattgcc
    14221 gaagacatag aaatgcgcac tatgatctat atactgacca ggccaatgcc gcgctctgcc
    14281 tgggttctgg gcagattctg cgcatatatt tttgtcgcat cgctgattct tatcagctcc
    14341 gctgtgctca ctttcgctgc ctcctgtgtc cattccaacc tgtctctaat gagtgcagga
    14401 gacattaaac tgctgcttca ttattgcggc gtcatggtgc tagggctctt tgcttatggt
    14461 gccttctcag tgtttcttgg cgcatttacc agacgaccga ttgtgtttgg agtgattctc
    14521 ttttacggat ggcagcacct tgcaaccctc attcctggtc tggtagactt ttttacaatt
    14581 aagaaatata ccactgccat gctgccgtta ttggctacac aacgacacaa ccccgtcatc
    14641 gagactgtgc tcggcaacat tcagcgggaa gtctttttag tgggtatgcc caaagcactg
    14701 gctgctttag tgtttatcac cgtggtatcg cttcttttaa ccatttatgc cacttaccgg
    14761 cgtgaatatg cttccgaccg tgcggctggc ggatgagaat cttcttatga aaggaagaga
    14821 aatttgtgag tgggaagtgg gaaccggata cgagccaatt ttatgcgtta tcaggggaag
    14881 aaaacagcgc tgcagattct attcaaaccg cgctggcaga ctttgagcat tattttaatg
    14941 cctttttaaa agcgctggat ggccaggccg agctgaaaga ccttcttctg gatggtactg
    15001 aagaatggcg aaggcttctc tctcaaaaac ttctcacgca gatttcagaa aaagcctgtc
    15061 ttattgttgt atttgcaggg ggcactaaca gcggaaaaag tacacttttt aatctgactc
    15121 tacaaaaaaa actcagtgaa gttagaagta ccgcggctgc gacaaaagcg ccagttcttg
    15181 cagtagcacc agaaaggctt cctgatgcca tcaacaataa gattcttgta gattttcacg
    15241 cacggaatca ggcttttccg gggcaaagtg aaaacccgca ggaaaaagaa gatcttctct
    15301 atgtggcgtc agctgaaaat ctgccttttt ccatggctct catggatact ccggacgtag
    15361 actctgttat tgaaaaaaac tggtctatag cagaaaagat tcgggcagct ggcgatgtaa
    15421 tgattgccgt ccttacccct caaaaatata acgatgaaaa aaatgtagct tttttccgta
    15481 tcgcacacca atcgggacgc atcattgttc cgctgatgaa cagagtggag ccctcccata
    15541 ctccggacat aacaaaagcg cagctgaatc agtttatggg agcaattggg tttaccgcag
    15601 aacgcgcttt tacagtgccc aaagattact ccctcgagtc cttgcctgat ctccccatca
    15661 tggacctgga aaaccaaata tcgttgcttg attatttgaa gcggatggat atgggcaccg
    15721 taaaaataca ggtctacaaa gcgtcgcttg aatatttttt agagcaaacc gcatcttttc
    15781 tggaacgatg caaaaatgcg gaagcacaga tcagagcttt taatgcggaa tgcctggaag
    15841 aggcagaaaa agctgctgcg gtattttcac ctgaacgccg gagcaatata aaaacaggca
    15901 tacgccagtg cattcaaaca agcagaagcg ttattgttca aaaaatgtct tttcttttgg
    15961 ctcttctgtc tcctctttat ttgccgctga aaaattttat atttaaagac gacgtacaga
    16021 tcagccaggg caaaaataag aagcagtttc aggtggatag tgccaaagaa aatgctgcca
    16081 gagcgcagga cgccattcgt gacagtgttc tgatctattc caactggttt cgtaaaaaac
    16141 tgaatgaggc gacgaaggac acctcttttt tcttgcttaa agaaccccgg gacaattcag
    16201 caaatgttga ggtaattcgt aaaacgctgg aaaaagatgc cgaaggactt gttcccgaag
    16261 atacacttct cggcaccggg cttcaggagg gatggccgat tcaatcagga aaaataacag
    16321 cactgcagtg gatagatgcc acttttgtcc ttgcgcctta tatctttctg ttacttcttt
    16381 tatccaaagg gctgtcggca atttacccaa taatttgcat tcttcctgtt ttttatatgc
    16441 tcctgcatgt gcgccgcata attgagcgta gcctggaaaa gcaaataaga gtttttttaa
    16501 gcccggcgta ccaccggcaa aaagaacgat taaaggagga attgtataga gtcgttgcta
    16561 aaccgatagt aggagcggtt gaaagcgccc tggctctttt tgatgaggag catataaaac
    16621 aacttgggag ctgcaggg
//
LOCUS       DAOPWB010000071        16618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3528_length_16618_cov_4.829862, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000071 DAOPWB010000000
VERSION     DAOPWB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16618
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3528_length_16618_cov_4.829862"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..745
                     /locus_tag="PKN92_07905"
     CDS             71..745
                     /locus_tag="PKN92_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNZ48755.1"
                     /translation="MIHFSLMQEAGLSVAAMSDKSDGDCGSRDEAVPNTEAFMTLAGA
                     PDVRPVRVRQVHGIAVVCAEEARGASLEADGILLGKAGIAAGVSVADCAPVLLFDPLV
                     RAGCLLHAGREGSFRNIAGTGVALLTHRFGSQPSSLVALIGPCAHGCCYEVSPELAAQ
                     WHRAGYACRDRFLDIPGTNSAQLEKAGVFRHNIHIVPHCTVCGGVFFSYRTDKTSNRN
                     LVVMMV"
     gene            941..1399
                     /gene="rimP"
                     /locus_tag="PKN92_07910"
     CDS             941..1399
                     /gene="rimP"
                     /locus_tag="PKN92_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNZ48756.1"
                     /translation="METDALVRQLWQLLEGDLQQLGFELVEVEMSRQGGAPLLRVFID
                     KEGSGITMDECTRASQMMDPLLDSADFLDGRYMLEVSSPGISRPLRKASHFERYCGQP
                     VRVETRAPVDGRSRFRGVLRALDGDLIRIQMGNAEVALHLENIKKARLDL"
     gene            1424..2584
                     /gene="nusA"
                     /locus_tag="PKN92_07915"
     CDS             1424..2584
                     /gene="nusA"
                     /locus_tag="PKN92_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNZ48757.1"
                     /translation="MTENMKMILQQLEAEKSLDMDTLIEAIRGAIETAAGRAMHKKVA
                     HVSVEVERQPLSFKVFEIRLVTETVQDPSSEIALEDALKLNPDVVVGNKLKVPAAPAD
                     FGRIAAQAARQMIAQKIREAERERIFQDFKQHEGDLVVGSVKNIDDHRNLFITVGQVE
                     AVLRYREQSPRERYKVGDRIRAIILEVDRASKGAKVILSRNAPELVEALFEHEVPEIR
                     DGTVKIKAIARDAGNRTKIAVASNDPNVDAVGACVGLKGSRVRAVVEELNGEKIDVIR
                     WNSDPMQLCINALNPADILDIRMNEDMKRIHVQVPQEQLSLAIGKSGQNARLASQLIG
                     WNIDIRGDSESLSQMEGNVPENHKEKEKDGPENELSEKGDGGSETVSDTDTE"
     gene            2667..5624
                     /gene="infB"
                     /locus_tag="PKN92_07920"
     CDS             2667..5624
                     /gene="infB"
                     /locus_tag="PKN92_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNZ48758.1"
                     /translation="MHTLASLQGLSWKPEKALEMLRQLKIEVESIDASIPGNIVDLLF
                     DIDDDPSIFDKMLKKIKAQEKEEEEAERKKEKEAAKKKAEAAKKKAEAKAKTAAKAKP
                     KAKPKAKEETKAKAPAKAKSKAKPKAKADAIVEEEAAESAVAEIIDEEVTPAPQADEA
                     EILAVPEPEVAEVVETPAAEAEEAPADETPEPSAPEPEEEHAHPAKVVAEIIEESEEE
                     RLADLERRESRIQSLEETQGTGVLAQAEKRHEEEQRRQARRLQQPPEPQGPVLSTIQA
                     DPEVLRAVLERDAERKRRASEPPAPRGGRRVADAGKAAEPETAKANLIAQAPLKEVSA
                     EGDKDRRKKVKKVDRTRLREDGMRRETEAAIREFRSGTGFSGSRKKRKRKREEQAIST
                     PESSIGGTIEVETEVTVEALAGLMNLPVNEVILALMEDNVLLTKNQSLDIEMVRHIAS
                     KFNFEVEVAIPEEEAIFKEEEDDPADLIPRPPVVTVMGHVDHGKTSLLDTIRSTKVVE
                     GEAGGITQHIAAYDIKVQDNKVTFLDTPGHQAFAQMRARGAHVTDVVVLVVAANDGVK
                     PQTVEAIHHAKAADVPIVVAINKCDVESAQPDRIRQELTQYELLDEAWGGKTIMKNIS
                     AKMNQGVDELMEMLVLQAEMLELRANPKKRAKGTIIESELSMGLGPVAWVLVQEGTLR
                     IGDAFLAGTAHGRVRSMIDCRGVEVQEAGPSTPVVVTGFNMPADAGEVFIVTPDERIA
                     RDVAEKRLHLARSKRGPAVRQMSLEDFHARMEGQEQKVLNLIIKADVQGSVDALQNMF
                     SADLRTAEVSIHIVHAGVGGVTESDVDLAISSQSIIIGFNVTANAKVKKIAEQTGVDI
                     RQYRVIYDALNEVRSAVEGMLTPESKEVVVGHAEVRNVFTSSALGKIAGCYQTDGETT
                     RNALARLVRDGVIKYQGKIASLRRHKDDVKAITAGFECGIKLEHYEDIQEGDIIETYK
                     IEQIAKTLDTDAL"
     gene            5621..5902
                     /locus_tag="PKN92_07925"
     CDS             5621..5902
                     /locus_tag="PKN92_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNZ48759.1"
                     /translation="MTIGVLYLDLAMPESRSLKDRRRAVKSLKERIQNRFNASVVELG
                     DPDLWQSAMLAVCIIGNDVRHVNSQLSSVAAFAGQDHRAVLTDYRIEML"
     gene            5899..7251
                     /gene="rbfA"
                     /locus_tag="PKN92_07930"
     CDS             5899..7251
                     /gene="rbfA"
                     /locus_tag="PKN92_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNZ48760.1"
                     /translation="MNGKGRIARVGALIQEEVARLISRGIKDPRIGFVSVMGVRMSKD
                     LRYANVYVSLMGSESERKSSLIGLRSSAGWVRREVGRHLHMRVLPEIRFFADTSLDEV
                     YHLESVFEEINAEKQRQPLLQLNHFEVITELRNARSFLLAVHENPDGDAIGSMLALRL
                     LLQQLGKSSITCVCADPVPSMFHGLPGAAAIVNAESDADTDDYDLGILIDCGTFSRIG
                     AAADFFASEKRLLILDHHQEPGESGSVGLVDAGFAATGELIAELYQAAEVPFTADAAA
                     CLYAAISCDTGCFRFANTTARTHRLAAELMETGIAIDELNARFFSDMSPQKFEVMRRV
                     LERVTLYHEGAVALSWLTAADLQEIGAVNSDSENLVNLWQSIRGVQLAIFLKQFDEGE
                     TRVSFRSTASFDSAAFLRTLGGGGHAMAAGATLELPLDEARARLLEALAAWPESPWAR
                     "
     gene            7305..8201
                     /gene="truB"
                     /locus_tag="PKN92_07935"
     CDS             7305..8201
                     /gene="truB"
                     /locus_tag="PKN92_07935"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNZ48761.1"
                     /translation="MQLNGILLVDKPQGITSHDVVDVLRKATGIRRIGHTGTLDPRAT
                     GLLVMCLGQATRLSQHFSGLDKTYEGALRLGIITDSHDLDGTIVEERPLPEGLSREQV
                     QAVCHEFLGDIEQIPPMVSAIKIGGRRLYKIAREGGEIERPPRKVRVDQFEVLRWELP
                     EAAIRVSCSSGAYVRTLCHDAGMRLGCGGVLAQLRRTRVGRYAIEDALPLDQYDSAER
                     VAERLISMNDALHLPVVSVDAIQENILMSGRALPARHLGISASATGEWVQVKNQRGKL
                     IALALMQDTPAGMCVQPKTVFV"
     gene            8224..9150
                     /locus_tag="PKN92_07940"
     CDS             8224..9150
                     /locus_tag="PKN92_07940"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNZ48762.1"
                     /translation="MHIITDARTTSERFPGLVLAVGCFDGVHLGHRQIVTALIEEAEK
                     IGGTPGIMTLDPNPRQFFSPGSAPNILTPGRVKCKLLAALGVQILYFLPFDARTAAMA
                     SGTFVQEILLDRCRIQGIIAGHDFAFGKGAAGNYEFLKESGEKYGFFTRQVPPLITGG
                     QRISSTLIRELILQGEVDTLPRYLGRHYSITGVVQSGRGIGRTLGFPTVNLDPGECAI
                     PAHGVYAARARLKGVAYQAAVNIGIAPTIQHDKPMIEAHLLDFSGNITGEEIEVIFYR
                     RMRPEQRFHSREALVDAIACDVEVVREYFSAL"
     gene            complement(9167..9976)
                     /locus_tag="PKN92_07945"
     CDS             complement(9167..9976)
                     /locus_tag="PKN92_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNZ48763.1"
                     /translation="MAFFTSLSPVMQAFIATLISAGTTALGALPVFFFKEINRKFLDG
                     MLGAAAGVMIAASFWSLLLPAIEIAKELNYTPWIPAALGFLSGGLCLLLIDKILPHLH
                     PSLAEADAEGLPTSWRRSTLLVLAITLHNFPEGLAVGVAFGAAAAGMPGATVAGAIAL
                     GIGICLQNFPEGTAVAMPLRREGLSPMKCFLLGSASGIVEPIAGVLGAGLVLLARPVL
                     PFTLAFAAGAMIFVVAEELIPESQREGDTDIPTIGLMCGFALMMLLDVAFG"
     gene            10348..11946
                     /gene="serA"
                     /locus_tag="PKN92_07950"
     CDS             10348..11946
                     /gene="serA"
                     /locus_tag="PKN92_07950"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNZ48764.1"
                     /translation="MLRVLLLDNLCEESIKLFEEAGIEADVKPPQTPEELAKIINNYD
                     GMVIRSATKVTAEALENSTRLKVIGRAGVGTDNVDKAAATKKGIVVMNTPGGNTISTC
                     EHAFALMLSMCRQIPRAHDSMEAGRWDRKQFMGTELKGKVLGVIGVGRIGGAIAKRAK
                     AFEMRVLAFDPILSPLKAESLGVELVGLNELLSTADFITVHVPKSDKTFNMIAKEQIE
                     LMKPNCRLINTARGGIINEQDLAEALRNNRIAGAALDVYTSEPFKDNPFLGLDNVIMT
                     PHLAASTNEAQLTVAVDVARQLIDYLTTGAIVNAVNVPSMDSDTRREMMPLLYLAERL
                     GLFQSLFVKGRPKSIEIEYIGDLGVTDTYAITTSIIAGFLRPMVETVNMISAPSQLQE
                     RGVEVSEKRSPVASSYAFEIGVTVTTDQETHNVRGTLFNRTDPRICTINGMRVDAKPE
                     GYMLVCTNEDKPLIIGRVCTAIGNAGVNIANLMLGRAGRGGQAVTVLNLDAPLDKETL
                     QKCIEVPHVTEVRQVALPEAQTVA"
     gene            12019..13230
                     /gene="icd"
                     /locus_tag="PKN92_07955"
     CDS             12019..13230
                     /gene="icd"
                     /locus_tag="PKN92_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNZ48765.1"
                     /translation="MPEGEKIQVDKSGRVQTPDTPIIGFIEGDGTGPDIWRASQFLFD
                     AAVKHCYGDKRKIAWMEIYAGEKANRHCGSYLPDETLDAIREYSIAIKGPLTTPVGGG
                     FRSLNVSLRQQLDLFACVRPVRHFEGVPSPVKEPEAVDMVIFRENTEDVYAGMEVEEG
                     TPETKKLIDFCTREFGWTIRPDSGIGIKPISISGTKRLVRAALNHAVQHNRKSVTLVH
                     KGNIQKFTEGAFRKWGYELVREEFADVAVGWDDCQGKPGDKILVKDAIADIFLQQILT
                     RPREFEVVATMNLNGDYFSDALAAQVGGIGIAPGANINYETGVAIFEATHGTAPKYAN
                     LDKVNPSSVVLSGVMMFEHMGWDEVSDAIITALTRTFKQKRVTYDFARLMSGATELKC
                     SEFAKAVAENF"
     gene            13386..14603
                     /locus_tag="PKN92_07960"
     CDS             13386..14603
                     /locus_tag="PKN92_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48766.1"
                     /translation="MKYLTFLSVFFCFLIASSVSAAEEIKYLTPEEFVIIPWGHIPDD
                     LEVLRTIRECGFNMAGLASDKTLDLVHEAGLKALLFNPDFHAHHVTLTLGKEEIQKRA
                     DAAAAVAPGHPALYGYFLRDEPGANDYPALALWREAFSRADPDTPAYINLFPNYASPD
                     QMNVPTYEEYVETFIATVKPEILSYDHYALMEGGVLRNGYFSNLETIRAAALRHDLPF
                     WNIVLSNAHFNYMEPTAAGFRFQLYTTLAYGAKGICYFTYFTPTTGNYRLGPIDQFGD
                     KTPAWHMLRNVNLQLHRIGPVYITLKSKNVFHHPNVPDGCRGMETSLYLSHLEGGDLM
                     AGEFENPEGQPFVLVVNKSLHHSCTVEAAFKEGNNILCVNAWTGLIHAWRGEHRWLAP
                     GQGMLMTISRLEE"
     gene            14651..15844
                     /locus_tag="PKN92_07965"
     CDS             14651..15844
                     /locus_tag="PKN92_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48767.1"
                     /translation="MKMLTPFFTVLSVALFAASLLSAQEYCALEKKLIEYGWDVPTPE
                     YIRDNIREMEKRPFDGVVFRLKGGGKVLDITPMTEEHYAGDYAAVAEIAWDKFTDNFV
                     IMWAATEQDWFNDDHWAIIENNTALVARAAKLAHCKGICFDPEPYGKNPWNYLETEHK
                     DSRSYLEYAGQARKRGAQFMRAIQREFPDPQILSLYQFALFGEFCKPMELEKLVEDLS
                     RHSYALYPPFLNGMLDAAGEGTVLSDGNENAYYYDRTERYYEAYHLMKQGVRYLVDPK
                     LWKVFDTKVLAGSSLYVDQYFGMRADPVLGHKMTPDERSLWFEHNVYHALRSTDRYAW
                     CYSEKMNWWTGETVPPGADEAIRRAREKLSRGETLGFDLAPIVAAAKERPFPEAPKEE
                     APPAP"
     gene            15998..16228
                     /gene="rpoZ"
                     /locus_tag="PKN92_07970"
     CDS             15998..16228
                     /gene="rpoZ"
                     /locus_tag="PKN92_07970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNZ48768.1"
                     /translation="MPTPYCVDDFSQSFDSLYRLVIVAAARSNQLASAGTHGFGSLAK
                     PTVRALEEVIEGKLQWADTDELDEPPFDEEEE"
     gene            16235..>16618
                     /locus_tag="PKN92_07975"
     CDS             16235..>16618
                     /locus_tag="PKN92_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNZ48769.1"
                     /translation="MNRFFADKTVLLGITGSIAVFKACELVSRMRELGARVHCALTRS
                     GAEMIQPATLEALSGNPVITEMFGTRTGHTISHIAMTRQADLIMVAPATANILAKAAQ
                     GIGDDWLSTALLAARSPVLFAPAMNT"
BASE COUNT         3988 a         4223 c         4804 g         3603 t
ORIGIN      
        1 aaagcaggat ttccgaaatc gcggaaaaac gcgatttgag tcaatatgtg ctttaaggcg
       61 aaaatgcgcc atgatacact tttcgctcat gcaggaggcg gggctgtctg ttgccgcaat
      121 gtccgacaaa agcgacggag actgcggcag ccgtgatgag gcggtgccca atacagaagc
      181 gtttatgaca ttggccggtg cacccgacgt acgtcccgtc cgggtgcggc aggtacacgg
      241 cattgccgta gtatgtgccg aagaggcgcg cggcgcttct ttggaagcgg atggaatact
      301 cctgggcaaa gccgggatcg cagcaggggt cagtgtggcg gattgtgcgc ctgtgctgct
      361 ttttgatccc ttagtacggg caggctgcct tcttcatgcc ggtagggagg gaagtttccg
      421 taatattgcc gggacgggtg tggcacttct gacgcaccgt ttcgggtcgc agccttcttc
      481 tcttgtggcg ctgatcggac cctgcgccca tggctgctgc tacgaagtct ctccggagtt
      541 ggcggcgcag tggcataggg cggggtatgc ctgccgggac cgctttctcg atatccccgg
      601 cacaaacagt gcgcaacttg aaaaagcggg cgttttcagg cacaatattc atattgtgcc
      661 ccactgtact gtgtgcggcg gtgtgttttt cagttatcga accgacaaaa catccaaccg
      721 gaatcttgta gtaatgatgg tataacccca agcacaataa ggatctgctg aaacttcgta
      781 aatctgccct gattttacgg aggcgtaagc agaaatcggt tgcactttta ccggtatgta
      841 tgttattgta ctggtaacag atagcgaccg tgacaagtgg gtcttggccc actttttttg
      901 tttgtgtttt ttgtgtttca gggcaggaaa ggcatgttcc gtggaaacag atgccctggt
      961 gcgtcagctg tggcagttgc tggaaggaga cttgcagcaa ctcggtttcg agctggtgga
     1021 agtggaaatg tcccggcagg gcggtgcgcc gctgctgcgg gtttttatag ataaagaagg
     1081 ctcgggtatc acgatggacg aatgcacacg tgcatcccag atgatggacc cgctgctgga
     1141 cagtgccgat tttctggatg gtcgctacat gcttgaagtg tcatcgcccg gtatcagccg
     1201 gcccctgcgc aaagcatctc attttgaacg ctactgcggg cagcctgtcc gggtggaaac
     1261 ccgggcgccg gttgacggcc gctcccggtt tcgaggcgtt ctgcgtgcat tggatggcga
     1321 tcttatccgg atacagatgg gcaatgctga agtggctctg catctggaaa atatcaagaa
     1381 ggcacggctg gatctctgat ctgccccgcc tgaggagatt gtcttgacag aaaacatgaa
     1441 aatgatcctg caacagcttg aagccgagaa aagcctggat atggacacgc tgattgaagc
     1501 cattcgtggg gccattgaaa cggcagcggg tagagccatg cataaaaaag tggctcatgt
     1561 gtctgtggaa gttgaaagac agcctctgtc atttaaagtg tttgaaatca ggcttgtaac
     1621 ggaaacggtc caggatccca gttcagaaat cgctctcgaa gatgcattga aactgaatcc
     1681 cgacgtggtg gtgggcaaca aactgaaagt gcccgctgcc ccggcggatt tcgggcgtat
     1741 tgcagctcag gcggcgcgcc agatgatcgc ccagaaaatc cgggaagcgg aacgggaacg
     1801 gatctttcag gatttcaaac agcatgaagg cgatctggtc gtcggttccg taaagaatat
     1861 tgacgaccat cggaatctgt ttatcaccgt ggggcaggtg gaagcggtgc tgcgctacag
     1921 agagcagtcg cctcgggaac gatataaggt cggagacaga atccgcgcca tcattctgga
     1981 agtggaccgt gccagcaagg gtgccaaggt gatcctttca cgcaatgcgc ctgaactggt
     2041 tgaggcgttg ttcgaacacg aagttccgga aatcagggac ggcaccgtca agatcaaagc
     2101 aattgcgaga gatgcgggga accgcacaaa aatcgccgtg gcatccaatg atcccaatgt
     2161 ggatgcggta ggtgcctgtg tggggctgaa aggctcccgg gtgcgcgccg tcgtggaaga
     2221 gcttaacggc gagaaaatcg atgtgatccg ctggaacagc gatccgatgc agctgtgcat
     2281 caatgcgctg aaccccgccg acattctgga catccgcatg aatgaggaca tgaagcgcat
     2341 tcatgtgcag gtgccacagg aacagctgtc tcttgctata ggaaaatcag gacagaatgc
     2401 ccggcttgct tcacagctga ttggctggaa tatcgatata agaggggact ctgaatccct
     2461 cagtcagatg gaagggaatg tgcccgaaaa ccataaagag aaagaaaaag acggaccgga
     2521 gaatgaactt tccgaaaaag gtgacggcgg cagcgaaaca gtttctgaca cggatacaga
     2581 atagtttttt cgcctgttgt caaagtggcg tatcagctgc cgtaagtcaa aaagggcgca
     2641 aaggcagatc aggagataga aaaagaatgc atacccttgc aagtttacag ggattgagct
     2701 ggaagcctga aaaggcttta gagatgctcc gccaactgaa gatagaagtt gagagcatcg
     2761 atgcctctat tccgggcaat attgtggatc tgctttttga tatagacgac gatccctcta
     2821 tttttgacaa aatgctgaaa aaaataaagg cgcaggagaa agaagaagaa gaggcagaac
     2881 gaaagaaaga gaaggaggcg gcaaagaaaa aggcagaggc tgctaaaaag aaagcggaag
     2941 cgaaggctaa aaccgccgcc aaagccaagc ctaaggcgaa gcccaaggca aaagaagaaa
     3001 caaaggcaaa agcgccagcc aaggcaaaat ccaaagccaa acccaaagca aaagcggacg
     3061 caatagtaga ggaagaagcg gcagaaagtg ctgttgctga aatcattgat gaagaagtga
     3121 cacctgcccc gcaagcagac gaagcggaaa ttctggcggt acccgaaccg gaagtggcag
     3181 aagtggtgga aacgcctgct gctgaagctg aagaagcacc tgctgacgaa acaccggagc
     3241 cctccgcgcc ggaaccggaa gaggaacacg cgcaccctgc caaagtggtc gctgaaatca
     3301 tagaggaatc cgaagaagag cgcctcgccg atctggaacg ccgggagagc cggattcagt
     3361 cacttgaaga gactcaaggc acaggggtgc ttgctcaggc ggaaaaacgc cacgaagagg
     3421 agcagcgcag gcaggcacgg agactgcagc agcctccgga gccccaaggt cccgtcctct
     3481 cgaccattca ggccgatccc gaagtgctgc gtgctgttct tgaacgtgat gcagagcgta
     3541 aacgtcgtgc ttccgaaccg cccgcgcccc gtggtggcag acgggtcgct gatgccggaa
     3601 aagccgctga gccggaaacg gcaaaagcga atttgattgc ccaggcaccg ttgaaagagg
     3661 tttcagcgga gggcgacaaa gatcgtcgta aaaaggtgaa gaaagtagac cgtacgcgac
     3721 ttcgtgaaga tggtatgcgt cgtgaaacag aagcggcaat ccgggagttc cggagcggta
     3781 ccggatttag cggatcacgg aaaaaacgta aaagaaaacg tgaggaacaa gccatcagca
     3841 cacccgaatc ttcaatcggc ggcaccatcg aagtcgaaac agaagtaact gtcgaagccc
     3901 ttgccggtct catgaatctg cctgttaacg aagttattct ggcgctgatg gaagacaatg
     3961 tccttctgac gaaaaaccag agtctcgata ttgaaatggt tcgccacatt gcgtcgaaat
     4021 ttaattttga agtcgaagtg gcaattcccg aagaggaagc gattttcaaa gaagaggaag
     4081 acgatcctgc cgatctgatc ccgcgtccgc ctgtagttac ggtgatggga cacgtcgatc
     4141 atggaaagac atccctattg gatacaatcc ggtcgaccaa agtggtggaa ggagaagctg
     4201 gaggaattac ccagcacatt gctgcctacg acatcaaagt acaggataac aaagtcacat
     4261 ttctggacac gcccggacac caggcttttg cgcaaatgcg cgcccgcggc gcacatgtca
     4321 ccgatgtggt cgttctggtc gtggcagcga atgacggcgt gaagccccag acggtcgagg
     4381 cgatccatca tgccaaagcg gcggatgtgc ccattgtggt agccatcaac aagtgcgatg
     4441 tagaatcggc gcagcccgac cgcatccgcc aggagcttac ccagtatgag cttctggacg
     4501 aagcctgggg cggcaagacg attatgaaaa atatcagcgc caagatgaac cagggcgtgg
     4561 atgagctcat ggaaatgctt gtgcttcagg cggaaatgct ggaactgagg gccaatccca
     4621 agaagcgcgc caaaggcacc attatcgaat ctgaattgtc catgggactg gggcctgtgg
     4681 cgtgggtgct cgtgcaggaa ggaaccttgc ggatcggcga tgccttcctc gccggaacag
     4741 cccatgggcg tgtgcgttcc atgattgact gcagaggcgt ggaagtgcag gaagcgggac
     4801 cgtccacacc cgttgtcgtt accggtttca acatgcctgc cgatgcgggt gaagtgttta
     4861 ttgtcactcc ggacgaacgc attgcccggg acgtggcgga aaaacggctg catcttgccc
     4921 ggagcaagcg gggacccgcc gtgcgccaga tgtcgctcga agattttcat gcacgcatgg
     4981 aagggcagga acagaaagta cttaatctta ttatcaaggc cgatgtgcag ggctctgtgg
     5041 atgcgctgca gaacatgttc tccgctgatc tccgtacggc agaagtgtcc atccatatcg
     5101 tgcatgccgg ggtcggcggc gtgaccgagt ccgatgtgga tcttgccatc tccagccagt
     5161 ccatcattat cggcttcaat gttaccgcca atgccaaggt gaaaaagatt gcggaacaga
     5221 ccggcgtgga tatccggcag tacagggtga tttacgatgc cctgaacgaa gtccgcagcg
     5281 ctgtggaagg gatgctcaca ccggaaagca aagaagtggt ggtcggtcat gcagaagtgc
     5341 ggaacgtctt cacctcttcc gcattaggca agatcgccgg ctgttaccag accgacggtg
     5401 aaacgacccg caatgccctg gcgcggcttg tccgtgacgg cgtgatcaag tatcagggta
     5461 aaatcgcgtc tttgcggcgt cataaagatg acgtgaaagc gattaccgcc ggtttcgaat
     5521 gcggtatcaa actggaacac tatgaggata ttcaggaagg cgacatcatc gaaacataca
     5581 agatcgagca gatcgccaaa acactggaca ccgatgccct gtgaccatag gcgtgcttta
     5641 tcttgatctc gccatgccgg aatcgcgttc actcaaagac cggcggcgtg cagtaaagag
     5701 tctgaaagaa agaatacaga accgttttaa tgcttctgtt gtggagttgg gcgatccgga
     5761 cttgtggcaa agcgccatgc tggcagtatg cattatcgga aatgatgtcc gccatgttaa
     5821 cagccagctg agcagtgttg ccgcttttgc cggtcaggat catcgggctg tgctgacgga
     5881 ttacaggata gagatgttat gaatggtaaa ggcaggatcg cccgtgtcgg cgcattgatc
     5941 caggaagaag tggcacggct gatttcccgg gggatcaaag acccgcgtat cggattcgtt
     6001 tccgttatgg gggtacgcat gtcgaaggac ctgcgctatg ccaatgttta tgtaagcctc
     6061 atgggctctg aatccgaaag aaaaagctcg ctcatcgggc tgcggagcag cgcaggatgg
     6121 gtacgccgtg aagtgggcag gcatctgcat atgcgcgtgc tgccggagat ccgttttttt
     6181 gcggatacct cgctggatga agtgtatcat ctcgaaagcg tttttgagga aatcaatgcg
     6241 gaaaaacagc gccagccact gctccagctc aaccacttcg aagtaatcac ggagctgcgc
     6301 aatgcccggt cctttcttct cgccgtgcat gagaacccgg acggcgatgc cataggcagc
     6361 atgcttgcct tgcggcttct gttgcagcag ctgggaaaaa gcagcatcac ctgtgtctgt
     6421 gccgaccctg ttccttccat gtttcatgga cttcccggtg ccgctgccat cgtaaatgcc
     6481 gaatccgatg ccgatacaga tgactatgat ctcggcatac tgattgactg cggcaccttc
     6541 agccgtattg gtgcggctgc cgatttcttt gcctctgaaa aacgtctcct cattcttgat
     6601 caccatcagg aacccggtga gtccggatct gtcggccttg tcgatgcagg ctttgcagca
     6661 acaggcgagc tgattgctga actgtatcag gcggcagaag tgccttttac cgccgacgcc
     6721 gctgcgtgcc tttatgccgc catttcttgc gatacgggct gcttccgctt tgccaatacg
     6781 accgcgcgga cgcatcgtct tgctgctgaa ctgatggaaa caggcattgc gattgatgag
     6841 ctgaacgcac gttttttcag cgatatgtcc ccgcagaaat tcgaggtgat gcgtcgtgtg
     6901 ctggagcggg ttacactgta tcatgaggga gcagttgccc tttcctggct gacggctgcc
     6961 gacctgcagg agatcggtgc ggtgaactcc gactcggaaa atctggtcaa tctctggcag
     7021 agcatccgcg gcgtgcagct ggcaatcttt ttgaagcagt ttgacgaggg agaaacccgg
     7081 gtcagcttcc gctcaacggc atccttcgac agtgctgctt ttttgcggac gctgggaggc
     7141 ggcggacatg ccatggcggc aggtgccacc ttggaactcc ctctggacga ggcacgggca
     7201 cgccttcttg aagcactggc agcctggccg gagagcccct gggcacgctg aacagctgcg
     7261 ctgccctgac ctgaaccatt atctcttttc tggaattaca tctgatgcaa ctcaatggaa
     7321 tattgctggt cgataaaccg cagggtatta cttctcacga tgtggtggac gtactgcgca
     7381 aagcgacagg aatccgcagg ataggacata caggcacgct ggatccccgg gctacgggat
     7441 tgctggtcat gtgcctcggt caggcaaccc gtctgtccca gcatttctca gggctggaca
     7501 aaacctatga aggtgcgctt cgtcttggga tcatcacaga cagccatgat ctggacggca
     7561 ccatcgtgga ggagcggccg ctgcccgaag gactctcccg tgagcaggtg caggctgtct
     7621 gccatgaatt tctgggtgac atcgagcaga taccgcccat ggtcagcgcc ataaaaatcg
     7681 gagggcgccg cctgtacaag attgcccgtg aaggcggcga aattgaacgt ccgccccgga
     7741 aggtgcgtgt agatcagttt gaggtgttgc gttgggaact tccggaggct gccattcgcg
     7801 tctcctgttc cagcggtgcc tatgtccgga cactgtgtca tgatgcgggg atgcgtctgg
     7861 gatgcggcgg ggtgcttgca cagctgcgtc gcacgcgtgt gggtcggtat gcgattgaag
     7921 atgcgctgcc tctggatcag tatgattcgg cggagcgtgt ggctgagcgt cttatatcca
     7981 tgaatgatgc ccttcatctc cccgtcgttt ctgtggatgc gatacaggaa aatattctca
     8041 tgagcggccg cgccctgccg gcgcgtcatc ttgggatttc tgcttccgcc accggtgaat
     8101 gggtgcaggt caaaaaccag cgcgggaaac tgattgcgct ggcgcttatg caggatacgc
     8161 ctgcgggaat gtgcgtgcag ccgaaaactg tttttgtata aacagccagg agaagtgctt
     8221 cccatgcata tcatcaccga tgccagaaca accagtgaac gctttcccgg actggtgctg
     8281 gctgtcggct gtttcgacgg tgtgcatctg gggcaccggc aaattgtcac agcgctcata
     8341 gaagaagcgg aaaagatagg cggaacgccc ggcatcatga cgctggaccc caatccgcgg
     8401 cagttcttct cgccgggcag tgcccccaac atcctgacgc ccggccgggt caagtgcaaa
     8461 cttctcgccg cattgggtgt gcagattctg tatttcctcc cctttgatgc ccggacagcg
     8521 gcaatggcat ccggaacctt tgttcaggag attttgctgg atcgctgccg cattcaggga
     8581 atcattgccg gtcatgattt tgctttcggc aaaggcgcag cgggcaatta tgaatttctg
     8641 aaggaatcgg gtgagaagta cggttttttt acgaggcagg tgccccctct gattaccggg
     8701 ggacaacgta tcagcagcac attgatccgt gaactgatcc tgcagggcga agtggacacg
     8761 ctgccccgct acctgggccg tcattacagt attaccggtg tggtgcagtc cgggcgcggt
     8821 atcggcagaa cactgggctt cccgacggtg aatctggatc caggcgagtg cgccattccc
     8881 gcccacggcg tgtatgccgc ccgggcacgg cttaagggcg tagcgtatca ggcagcggtc
     8941 aatatcggaa tcgctcccac gatacagcat gataagccca tgatcgaagc gcatctgctg
     9001 gacttttccg gaaatatcac gggcgaggaa atagaggtga tcttctacag gcgtatgcgc
     9061 ccggaacaac gctttcattc ccgggaagcg ctggtggatg cgattgcctg tgatgtggaa
     9121 gtcgttcgcg aatacttttc cgccctgtga aacggatgca gcgctctcag ccgaaagcaa
     9181 catccagcag catcatcagt gcaaatccgc acatgagccc gatcgtggga atgtcggtgt
     9241 ctccttcgcg ctgactttca ggaatcagtt cttccgctac gacaaagatc atggcaccgg
     9301 cagcgaaggc aagcgtaaag ggaaggacag ggcgcgcaag aagtaccagc cccgcaccaa
     9361 gcacaccggc tatgggctcc acaataccgg aggcagaccc cagcaggaag catttcatcg
     9421 gcgacagccc ttcccgccgc agaggcatgg caacggcagt cccttcaggg aagttctgaa
     9481 ggcagatgcc gatgccgaga gcaatggcgc ccgccacagt ggcaccgggc attccggcgg
     9541 cggcagcgcc gaaagcaacc cccacagcga gtccttcagg aaaattgtgc agcgtaatgg
     9601 caaggacaag cagcgtactg cgccgccacg aagtaggaag cccctccgca tcagcctcgg
     9661 caagactcgg gtgcagatgg ggaaggattt tgtcaataag caaaagacac agcccgcccg
     9721 acaaaaaacc cagagcagca ggtatccagg gggtataatt gagctctttg gcaatttcaa
     9781 tggcgggaag caacagcgac cagaaactgg cggcgatcat cacgccggcg gcggcaccca
     9841 gcatcccgtc cagaaatttt cggttgattt ccttaaaaaa gaagactggc agcgcaccca
     9901 gtgcggtcgt accggcagaa atcagggtgg cgataaaagc ctgcatgaca ggactcagag
     9961 aagtaaaaaa agccatgact gttattcctg tatgactatg ctgagggcgt gtcggatctc
    10021 tttcccggaa aaaaacaggg atacatctat acgcatccgc ctggagcccc agttgaacag
    10081 aaattgtaat aaggacgggc ggataatgca aattttccgg aaaaagatcc ctttttcagg
    10141 tggttatttg cttttaaacc gcctgttcgg gtagtttatt ggcataaagg ggcgtttctt
    10201 tctgttatag agacatgcaa ggtggactgc aattgtatgt gtgagagagc gtaggggctt
    10261 ttctggcaat cgtttacaaa ctggtcgcgg tatatgagcc gctgtccaat cccatacacc
    10321 tttaatcctg tagatggaga atacccggtg ttgcgcgtat tgctgctgga caacttgtgt
    10381 gaagaaagca tcaaactctt cgaagaggcc ggcattgaag ccgatgtcaa accgccgcag
    10441 actccggaag agctcgctaa aattatcaac aattatgacg gtatggtcat ccgcagtgcg
    10501 accaaggtga ctgcggaggc actggaaaat tccacgcgcc tcaaagtgat cggtcgtgcc
    10561 ggggtcggta cggacaatgt ggacaaggcg gctgccacca aaaaaggcat cgtcgtcatg
    10621 aacacgcccg gcggcaatac catctccacc tgcgagcatg cctttgccct gatgctgtcc
    10681 atgtgccggc agattccacg cgcgcacgat tccatggaag cgggacgctg ggaccgcaaa
    10741 caattcatgg gcacggagct gaaaggcaaa gtgctgggcg tgatcggcgt cggacgtatc
    10801 ggcggtgcca ttgcgaaacg tgccaaggcc ttcgaaatgc gcgtgcttgc cttcgatcct
    10861 atcttaagcc ctctgaaagc tgaaagtctc ggggttgaac tggtcgggct caacgagctg
    10921 ctgtccacag ccgactttat cactgtgcat gtgcccaaat cggacaagac cttcaacatg
    10981 atcgccaaag agcagattga gctgatgaag cccaactgcc gcctcatcaa caccgcccgc
    11041 ggcggtatta tcaacgagca ggatctggcg gaagcgcttc gaaacaaccg gattgcaggt
    11101 gccgcactgg acgtttatac cagcgagccc tttaaagaca atcccttcct gggtctcgac
    11161 aatgtcatca tgaccccgca tctggccgcc tccaccaatg aagcgcagct gaccgtggcg
    11221 gtggatgtgg cacgccagct gatcgattac ctgaccacgg gtgccattgt caatgcggtt
    11281 aatgtgccca gcatggacag tgatacacgg cgggaaatga tgccgcttct gtacctcgct
    11341 gaacggctcg gcctgttcca gtcgcttttt gtaaaaggcc gtcccaaatc cattgagatc
    11401 gaatacatcg gcgacctggg tgttaccgat acctacgcca ttaccacctc gattatcgcc
    11461 ggatttctcc ggcccatggt ggaaacggtc aatatgatca gcgctccttc gcagctgcag
    11521 gagcggggcg ttgaagtgtc ggaaaaacga agccccgtcg cctccagtta tgccttcgaa
    11581 atcggcgtga cggtaaccac cgatcaggaa acacataatg tgcgcggcac actttttaac
    11641 aggacagatc cccgtatctg caccatcaac ggcatgcggg tggatgcgaa gccggaaggc
    11701 tatatgctgg tctgcaccaa tgaagacaaa ccgctcatta tcgggcgtgt ctgcaccgcc
    11761 atcggcaatg cgggcgtaaa tatcgccaat ctcatgctcg gccgtgccgg gcgtggcggt
    11821 caggcggtga cggtgctgaa tctggatgcg cctcttgata aagagacgct gcagaaatgc
    11881 attgaagtgc cccatgtgac cgaagtgcgc caggtggcac tgcctgaagc ccagacagtc
    11941 gcctgagcga atgcatcgta aaatactgtt ttaaccccgg taaaggagaa ctacgtgtcc
    12001 agctataccc atatcgtcgt gccggaaggc gaaaaaatcc aggtcgataa aagcgggcgg
    12061 gttcagaccc ccgacactcc gattattggt tttattgaag gcgacggcac cggtccggat
    12121 atatggcgtg cctctcaatt tctctttgac gctgctgtga agcattgcta cggcgataag
    12181 cgcaaaattg cctggatgga aatttatgcc ggggaaaaag ccaaccgcca ttgcggttct
    12241 tatctgcccg acgaaacgct ggatgccatc cgcgagtatt ccattgccat caaaggtccg
    12301 ctgaccaccc ctgtgggcgg cggtttccgg agcctcaatg taagtcttcg ccagcagctg
    12361 gatctgttcg cctgtgtgcg ccctgtccgg cattttgaag gggtgcccag tccggtcaaa
    12421 gagcctgagg ctgtggatat ggtgatattc cgcgaaaata ctgaagatgt ttatgcgggt
    12481 atggaagtgg aagaaggaac gccggagaca aaaaaactta tcgatttctg caccagagaa
    12541 ttcggctgga cgattcgccc cgattcaggt atcggcatta aacccatcag tatttccgga
    12601 acaaagcggc ttgtgcgtgc cgccctgaac catgcagtac agcacaaccg taaatcagtg
    12661 acgctggtgc acaaaggaaa cattcagaaa tttacggaag gcgctttcag aaaatggggc
    12721 tatgagctcg tgcgtgaaga attcgccgat gtggctgtcg gctgggatga ctgccagggc
    12781 aaaccgggcg ataaaattct tgtaaaagat gccattgccg atattttcct gcagcagatt
    12841 ctgacccgcc cccgggagtt tgaagtcgtt gccaccatga acctgaacgg cgactatttc
    12901 agtgatgcac tggcggcgca ggtcggcggt atcggcatcg ctccgggcgc aaatatcaac
    12961 tatgaaaccg gcgtcgccat ttttgaagcg acccatggta ccgctccgaa atatgccaat
    13021 ctggacaaag tgaatccgag cagcgtggtg ctgtccggcg tgatgatgtt cgagcatatg
    13081 ggctgggacg aggtctccga cgccattatt acagcactga cacgcacctt caaacagaag
    13141 cgggtcacct atgatttcgc ccgtctgatg tccggtgcca ccgagcttaa atgcagcgaa
    13201 tttgctaagg cggtcgctga aaacttttaa acgctgccct tcgtaacaat tgacacgccc
    13261 tcgctccgct tcggagtggg ggcgttttgt ttttgatgag cggggttaaa ggaaatgtag
    13321 tgatttcttt gcgtggtatg tcataatgag tgccggtatc acccctgatc agcgaaagga
    13381 atactatgaa gtatctgact tttctttctg ttttcttctg ttttctgatt gcttcttctg
    13441 tttccgctgc cgaagagatt aaatatctca caccggaaga gttcgtgatt attccctggg
    13501 ggcatattcc tgacgatctg gaagtgctgc gtaccatccg ggagtgcggc ttcaatatgg
    13561 cggggcttgc ttcggataaa acgctggatc ttgttcatga agccgggctg aaggcgctgc
    13621 tttttaatcc tgactttcat gcgcatcatg tcaccttgac gctgggtaaa gaggagattc
    13681 agaaacgggc ggatgcggct gccgctgtag cgccgggtca tcctgctctt tacgggtatt
    13741 ttctgcggga cgagccgggc gccaatgact atccggcgct tgccttatgg agagaagctt
    13801 tctcccgggc agacccggat accccggcgt atatcaatct ctttccgaat tatgcctctc
    13861 ccgatcagat gaatgtgccg acctacgaag aatatgtgga gacttttatc gcgacggtca
    13921 agccggaaat actcagttat gaccactatg cgctgatgga agggggagtg ctccggaacg
    13981 gctatttcag caatttggag accatccggg cggctgcact ccgtcatgac ctccccttct
    14041 ggaatatcgt tctttccaat gcgcacttta actatatgga gcccactgcg gcaggtttcc
    14101 gttttcagct ttatacgacc cttgcctatg gtgcgaaagg aatctgctac tttacctatt
    14161 tcacgcccac aacaggcaat taccggctgg gtcccatcga tcaattcgga gacaagacgc
    14221 cggcctggca tatgctccga aatgtcaatc tgcagctgca ccggatcggt cctgtttata
    14281 ttacgctgaa aagcaaaaat gttttccatc atcctaatgt gccggatggc tgcaggggca
    14341 tggaaacaag cttgtatctg tctcatctgg aaggcggtga tctgatggcg ggcgagtttg
    14401 aaaacccgga gggtcagcct tttgttctgg ttgtaaataa aagcctgcat cactcctgta
    14461 cagtggaggc ggcattcaag gaaggcaaca acatcctgtg cgtcaatgca tggacaggtc
    14521 tgattcatgc ctggcggggc gaacaccggt ggcttgcgcc cgggcagggc atgttgatga
    14581 ccatatcaag gcttgaagag taaaatagtc cggtgcctgt cgtcgtacaa aaacagaagg
    14641 aagacctgtc atgaaaatgc tgacaccctt ttttacagtc ctgtctgtcg cgctctttgc
    14701 tgcttcgctg ctgtcggcgc aggaatactg tgcactggaa aaaaagctga tcgaatacgg
    14761 ctgggatgtg ccgacgccgg agtatatccg tgacaatatc cgggaaatgg agaaacggcc
    14821 ctttgacggt gttgttttcc ggttgaaagg cggggggaaa gtcttggaca tcacgccgat
    14881 gacggaagag cattacgccg gcgattatgc ggctgtcgcc gagattgcct gggacaaatt
    14941 cacggataat tttgtcatta tgtgggcggc aacggagcag gattggttta atgatgacca
    15001 ttgggcgatc atcgagaaca atacagcgct ggttgcccgt gcggcgaaac tggcgcattg
    15061 caaaggaatc tgctttgatc ctgagcccta cgggaaaaat ccatggaatt atctggagac
    15121 agagcataag gactcccgaa gttatctgga atatgcggga caggcacgaa aacggggcgc
    15181 tcagttcatg cgtgccattc agcgggaatt ccctgatccg caaatccttt ccctttatca
    15241 gtttgccctt ttcggcgagt tctgcaagcc catggaactg gaaaaactcg tggaagatct
    15301 gagtaggcac agttatgccc tgtacccgcc ctttctgaac ggcatgctgg atgccgccgg
    15361 tgagggcacc gtgctcagtg acggcaacga aaatgcctat tattatgacc ggacagagcg
    15421 ctattacgag gcctatcatc tcatgaaaca gggcgtccgg tatctggtgg atccgaagtt
    15481 gtggaaggtc tttgatacaa aggtactggc agggtcgtcc ctttatgtgg atcagtattt
    15541 cgggatgcgt gccgatccgg tgctcgggca taaaatgacg cccgacgagc gctcactctg
    15601 gtttgagcac aatgtctatc acgccttgcg cagcacagac cggtatgcct ggtgttacag
    15661 cgaaaagatg aactggtgga caggggaaac ggtgccgccc ggtgcagatg aagcaattcg
    15721 gcgtgcccgt gagaagctga gccggggtga aacgctcggc tttgacctgg ctcccatcgt
    15781 tgctgccgct aaagaacgcc ccttcccgga agcaccaaaa gaagaggctc cgcctgctcc
    15841 ctgacggata tcggagcgat atttccgttt ttttgcgcct ttatgctata ataaagtgcg
    15901 tattgttggt gtttgtgtgt ttctgcagag aaaaagcgaa gcccggcgac ctgccggttc
    15961 tcaaaaaaag cgtgttatat acacaggagt acccggaatg cccacccctt attgtgttga
    16021 cgatttctca caaagctttg acagtctcta ccggttggtc attgtggctg ccgcacgctc
    16081 caaccagctt gccagtgccg gtacccatgg cttcggcagc cttgccaagc ccacagtaag
    16141 agcgctggaa gaagtgattg agggcaaact tcaatgggca gatacagacg agctcgatga
    16201 gcctcctttt gacgaagaag aagaataaac gtccatgaac cgttttttcg ccgataaaac
    16261 ggtgcttctg gggattaccg gttccatagc ggtgttcaaa gcctgcgaac tggtttccag
    16321 aatgcgtgaa ctgggcgccc gggtacactg tgcgctcacc cgttccggcg ctgagatgat
    16381 acagcccgcc acactggagg ctttatcagg caatcccgtc attacggaga tgttcggcac
    16441 ccgtaccggt cataccatca gtcatatcgc catgacacgg caggcagatc tcattatggt
    16501 tgctccggca acagcgaata ttcttgccaa agcggcccag ggcatcggtg atgactggct
    16561 gagcacggcg cttcttgcgg cgcgttcccc tgtccttttt gcgcccgcca tgaataca
//
LOCUS       DAOPWB010000072        16608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3533_length_16608_cov_5.851507, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000072 DAOPWB010000000
VERSION     DAOPWB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 16608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16608
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3533_length_16608_cov_5.851507"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            868..2691
                     /locus_tag="PKN92_07980"
     CDS             868..2691
                     /locus_tag="PKN92_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNZ48770.1"
                     /translation="MSTFDSTKTSLESLLKEICDGRIQLPDFQRGWVWDDDHIRDLLV
                     SIARSFPVGAIMLLETGGPVRFQTRPVEGLEKIVPTEHAPEKLILDGQQRLTTLTQAL
                     ALAAPVETRTGQGKKIKRHYYFNIRQALSMPQDLSSAVIGVDENRQIRSNFGRDIELD
                     LSTREKECQELYFPCDQTMNSDEWEETLHRVAPEYFELYMGFRRSVLKSFREYQIPVI
                     QLHKETTKEAVCLVFEKVNTGGVQLSVFELITASYAAEGYNLRDDWYGSDIRKVDSRR
                     EGLVAEPLLSEIQATEFLQGVTLLHTFERKKSDSSSGRTGKEVRPVSAKRGDVLELPL
                     NAWLTWADKLEAGFRLAARFLRKESFYSSRELPYSTQIVPLAAVLTHLGEKWLEPRIY
                     DKLTRWFWCGVLGELYGGAVETRIANDFEQLLEWIKKDDQEPRTVYDASFQPGRFDTL
                     RSRLSAAYKGINVLVLREGARDWLWKASIHELDVDEVKLDIHHIFPRNWCRKRGIPMG
                     SYDSILNKTPLSYKANRKIGGEAPSAYLPKIQSEKHLQLSDQQMDALLESHALNPALL
                     RADDYEAFIADRRRRLSALVSKAMGKPVSQIGESEVYEEAE"
     gene            2915..3679
                     /locus_tag="PKN92_07985"
     CDS             2915..3679
                     /locus_tag="PKN92_07985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ48771.1"
                     /translation="MSRPADTFSQLFFEVFESLPRQGPGSRNSAAKALSFCRELPSGP
                     LILDLGCGSGSQTFHLAELTSGSVVALDSHAPSIDKLRAAAASRGYDSRIRAVVGDMS
                     KPDLPRDSFDLIWSEGAFYNIGIACALDVCRPLLRAGGYLAFTDAVWRKENPPASVKA
                     VFDQDYPGMGRAEEIVALIQKAGFALIGQLMLPDEDWWDDFYTPMELRIDELRQKYSG
                     DKEALAALAALAEEPEMHRRHAACYGYSFFIARLAK"
     gene            3849..4868
                     /locus_tag="PKN92_07990"
     CDS             3849..4868
                     /locus_tag="PKN92_07990"
                     /inference="COORDINATES: protein motif:HMM:NF017843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HNZ48772.1"
                     /translation="MARLSQIDEVMFPVESCPVFACVPTDQGERHMPASDTKAIVNMN
                     THKVVGVVSKGYRLVTNHQALQWAYQCCETVFPETKPNEWLVKNADSPKTGSHCFIDL
                     VHHTALLDFCFVPASNRPDTYGPFIRLTNSYNGSRALTFHIGFFRKVCSNGLILPKAV
                     VSFRFNHQRREIGEYLNFAVANNQVANLKKALTIYLGALQDCKVPHNELVRLFCLILL
                     IRQPAEVKPDDPMRIEWEYVYSHITGLCRKYQNDFGNTAYAVFNAATEFASHPIQSRC
                     VRRERHSYQRLAGTWLAQFQEECQQPGFSIPDYIEKMTRKKKSSTREPPLFQNIDMPV
                     LPGFL"
     gene            complement(4912..5637)
                     /locus_tag="PKN92_07995"
     CDS             complement(4912..5637)
                     /locus_tag="PKN92_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ48773.1"
                     /translation="MKGNVFIAGASGAVGRRLCKLLVADGWSVTGTTRSHEKAQLLRG
                     LGVTPAVVDVFDEALLTELVKKSEPAVVVHQLTDLPPALDPAKMADALIRNARIREEG
                     TRNLIAAATAAGAERMVAQSLGFVYAPGPKPYTEESPLGAADPSFTGTVRAVISLEEQ
                     VLNAPFPGIVLRYGKFYGPGTGFDEAAKDGPLHVDDAADAARRALTKGQAGVYNIAED
                     DGTVSISKAQTELGWAPGFRIHP"
     gene            complement(5650..6774)
                     /locus_tag="PKN92_08000"
     CDS             complement(5650..6774)
                     /locus_tag="PKN92_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNZ48774.1"
                     /translation="MHTDKMRLCIIIAGIILSMAVPVPADENGAMTATRFDGMNLDNA
                     SPDPETTRAGEALALGIAPDVIGTAAPAAVMKTIDGQQIDLSAIYGRKPVYLKFWATW
                     CIPCRQQMPAFEEIYRTLGDRIQIAAIDIGYSDDEASVRAFREKLGLTLPIVMDDGSL
                     AKGFHLTVTPQHVLIGKDGRFKYIGHADNEALAEALKQVLQEEADPAELAPERLEHEK
                     VIQVGEKVPDLSVATLQNGSLPLKAESGHLLAVQFFSTWCEWYLESSRPDTSQACTRV
                     REAIETMKSTVPEVDWIGIAGGPWTTKEDLAEYGKNYQVTIPLALDEGGELNRRFGIQ
                     EIPTVALIDDTQRLIELIAPAEKDLQGAIQKALGKAADNH"
     gene            7112..8158
                     /locus_tag="PKN92_08005"
     CDS             7112..8158
                     /locus_tag="PKN92_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HNZ48775.1"
                     /translation="MITGDENDFSEIVIYQSEDGEISLDVVLEDETVWLSQEQMGKLF
                     GKDKRTISEHIQSIFSEGELDESGVVRNFRTATRHGAMPDRKQTHSVRFYNLDVIISV
                     GYRVRSLQGTRFRIWATRRLREYIVKGFALNDDRFKSGHSMNYFEQLQERIRAIRLSE
                     RFFYQKIKDIYTTSIDYDPKDEKTLTFFKTVQNKLLWAISEQTAAELVYRRTNAALPL
                     MGMQSFDKNTSVGITKKDVSIAKNYLDEEEMKLLGLLVEQYLAFAETMAQQHRPMTMS
                     DWIDRLDAIISLNGRELLTHAGRISHEMAVEKSSIEYKKYQELKRTVEREESIKELES
                     DLNRLEFHKKPDDA"
     gene            complement(8325..9737)
                     /locus_tag="PKN92_08010"
     CDS             complement(8325..9737)
                     /locus_tag="PKN92_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ48776.1"
                     /translation="MKSFDLEIISGSRLRSVWKLAWPLVCLNLVNGTHGIVDHILVGH
                     FIVSDNNAANAAIGVAWQVFLVLVVFITSVFHGMNVLISRNAGRQDRERLSRVFYTAF
                     MASFVALTFIFGPIGYLLAPGLLRLMNVPPEVALHCLPYLRILFVCGTPLFLMFLLTG
                     AFHASGDPKLPLKLGVLTTVLNIVISTVLITGLGIFTPLGIRGAAFGTVLAPMVSGLL
                     GIRLIYKRRTIIQPPERHRLMPDMRLLITMMRIGIPSGLQGVLLNIGGVMLLWYISLL
                     EYSAAAQAAYIICYTQLFSLVSWISFGLRGASGTMIGQNIGAGNPKRGKEGVLIAGAL
                     AGFWAALVGILYISIPGTLLSLFNVTDGLVYDYAVTLLRYLSVSGIVLGIAQALTGGL
                     QGAGATRPPMLIAFVTQIVTLLGFCQIFLSLNRLTPEVIWSAILLSHILRLLLSFLVF
                     RRKGWEHTIAHLDTGSKPLV"
     gene            9915..10973
                     /gene="ychF"
                     /locus_tag="PKN92_08015"
     CDS             9915..10973
                     /gene="ychF"
                     /locus_tag="PKN92_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNZ48777.1"
                     /translation="MKVGLIGFARSGKTTVFNAITGARAEVGAYGSRDANIAVIKVPD
                     ARVDALAEIYKPKKKTYAEIEFVDIAPAEGSDQDRALDSAALTLLKNADALAHVVRAF
                     KNDNVMHPRDTVNPVRDVQSLEEELQLIDLIVLEKRVERLEKEHRKDQEYELMVRCKA
                     HLESGKPLRTLDFSAQDALLLRSYSLLSQKPMMLLCNSGDDGLGDADPGGLSEIASAL
                     GLDLISFCGALEMEVNGLPEEERAAFREELGMGEESRTRFIQFAYSMLGLISFLTAGE
                     PEVRAWTIRKGDHAVDAAGVIHSDIKRGFIRAETVAYADFMEAGSMAKAKEKGKVRLE
                     GKDYIVQDGDMMLFRFNV"
     gene            complement(10970..12700)
                     /locus_tag="PKN92_08020"
     CDS             complement(10970..12700)
                     /locus_tag="PKN92_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48778.1"
                     /translation="MKTRKIPKRTLLFITVLLTVPGAIPVALILPGIHIFGGSGRFRM
                     HTMEPGEIMPEPDTEMNGSMPPSPLPPVGLQLLDIVTAQVRPEEATERTFSPKNFLAW
                     LASGKDTERINALLRERRPHAGVGSSWLLRKGDYDFSLAAWTTLLYLFGDQPERLYPE
                     TKAHLLDVLLTESGGKPKTTVPGTCRLITETENHILLAESSRYLKNQWLHLNGTEAEQ
                     ADPRWDNARNGLESWLLGFLEAVLTEGIYEFNSRPYSGYTIHALLNLEGFAASPAVRQ
                     KARLILDRQNLTYALGSLQLRRFPPFRRQYRRADTQELDSDPHTAFFYAWTGVEPDDP
                     RVIPWARNYSDWSLIASLLPYRLPEALRQWALEKKEPYFVRFSHGASGCPEIYSAGPG
                     WLLTAGGFNRGLRSLVVAMPITLMLEDGQRYLDGCIHLNGAGKRRSWNATGVYTDFAC
                     IRGTAHVPEGWTPEAAAGGWRIFRAPITQPLVIALYENQDQVCLLALFHEADLSADAL
                     LAWIQEANPEEAALSGRFARPGGTTLEYDLDAKAGCWVMKEENGTPFDRNCDRWEQIG
                     GTAPHLSFRR"
     gene            complement(12726..13349)
                     /locus_tag="PKN92_08025"
     CDS             complement(12726..13349)
                     /locus_tag="PKN92_08025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNZ48779.1"
                     /translation="MKTRNGLSVLLASASPRRKALLKALGASFDIWAAQAEEIDEGES
                     PESIVIGNAERKCRAAAEAHPCHDLIISADTLVFLNGAVLSKPADLDEARSMLRRLSG
                     NTHQVVTGLAVYHRASEQLITDSETTGVRFRELSEAEIDCFVEIVRPLDRAGAYTVDG
                     PGSLLVAGYDGCYQNVLGLPLVRLDLLLRKVNCSLFQWINPKEARFL"
     gene            13492..13638
                     /locus_tag="PKN92_08030"
     CDS             13492..13638
                     /locus_tag="PKN92_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48780.1"
                     /translation="MKHVKQVTQPRKAMSTTDILTLVGSILSGVGTILITVAQSVGGS
                     KSVG"
     gene            complement(13781..14362)
                     /locus_tag="PKN92_08035"
     CDS             complement(13781..14362)
                     /locus_tag="PKN92_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48781.1"
                     /translation="MMMLLFVLSAVSQTDALFTGTVPAEIPTFSVTLSGAAPFYAAMQ
                     VGGQWVTDRNQEIKPTQEVEFVPDIPWRTHEVVRTSMRRAAVSYEAPAMRRERLAKTW
                     KSMGYTILETASGWSPVKEKDIQLAERAKSMAENLEKRLRPVSDAPAAAEAAAVPEDA
                     GAAMRPAILWTLRAAIVLVGLSLAGALFYFTRP"
     gene            14580..15992
                     /gene="leuC"
                     /locus_tag="PKN92_08040"
     CDS             14580..15992
                     /gene="leuC"
                     /locus_tag="PKN92_08040"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNZ48782.1"
                     /translation="MKKNIYQKIWEAHLVCEPEGEDAILYIDRHYIHEVTSPQAFEGL
                     RLSDRKVRRPDLTYATMDHNIPTTDRSLPIADILSRAQVETLKKNCAQFNIECFDMYD
                     PRNGIVHVVGPELGLTQPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLRQ
                     KASKTMEVRINGTLPPGITSKDIVLAIIGKIGTSGGTGYVIEYTGEAIRNLSMEARMT
                     ICNMSIEAGARAGLISPDDTTIAFLKGRAMLPKDRSFEALAEEWKQWASDPGCSYDTL
                     VELNAEDIEPQVSWGTSPEMVGGISGTTPVLADVADVNKRHAYGKALEYMGLEEGMPL
                     QSIAIDTVFIGSCTNGRIEDLREAAKVLRGYKKADSVQRALIVPGSQAVKRQAEEEGL
                     DRVFRDAGFEWRESGCSMCLAMNDDKLAPGERCASTSNRNFEGRQGKGGRTHLVSPAM
                     AAAAAVKGVFTDIRNWKYQD"
     gene            16058..>16608
                     /gene="leuD"
                     /locus_tag="PKN92_08045"
     CDS             16058..>16608
                     /gene="leuD"
                     /locus_tag="PKN92_08045"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNZ48783.1"
                     /translation="MNPFTIHKGLVAPLDAMHVDTDQIIPKQFLKRIERTGYEDVLFF
                     DWRYLDDGKTPNPEFEMNAPRYQGASILLARDNFGCGSSREHAPWALFDYGIRSIIAP
                     SFADIFFNNCFNNGMLPIRLSADLVEELFQQTRAAEGYSLEINLPGQKIIRPDGSAIS
                     FEITPFLKQRLLNGWDQIGLTLR"
BASE COUNT         3930 a         4227 c         4596 g         3855 t
ORIGIN      
        1 gagcagcccg ccgcgtgctg cgctcgtacc tggactgggg cgtgcttcag gagacgggga
       61 agcagggagt gtattctgcc gcgaagcctt tacccgtaga ggaggctcag gcaatcgctt
      121 ggttagcaga agctgctctt cactcgcgag gcaacggttc agcatcattg ggagagattc
      181 tcgaatcccc ggcattcttc ccttttcaga tcgcgccaat tcaccccagt ctcttggcga
      241 ccgcgtcttc tcgactcgat gtactgcgtc agggattgga cgagactctg gtaatgttcc
      301 gtagtaccgc tgaaaggagg aggtgagacc tcgacgccac caactatgtg gatgtcatta
      361 ttgcgctcgg ttcggttttc tccaggcttc cttccgggta aagatgcagc acaagttggc
      421 cagtggttat tggtgtggag gccgcacggc gagacctcaa gggcgtagtc ggcggcgagt
      481 cgatgccaag cgatgcctat tctcagctgc tctcgatgaa tactcatcaa tacggttgcg
      541 tctgggaagc cagatcattc ccgatgcctt ggcgtcggtt cgttttttaa cgctccggtc
      601 tcatccagac cggggtgttt ttttattttc ggctgacttt tcgctttgat ccaggcaagt
      661 ttctctgtaa cgtcctgtgt ctgaaagcac cccgatggac ttgcgacaaa cctgtggatg
      721 caattcgttt ttatttctgg gttcagactg tcaacgctac ggaaaaaaga aaaagcagtt
      781 actctatgta aagcaatatt gtttatgata gtgaccgctg aaaataattt gcgctttctg
      841 aagagacaac attaaaaagg agagaatatg agcacctttg acagcaccaa aacatcactt
      901 gaatctttgc tgaaggaaat atgtgatggc cggattcagc ttccggattt ccagcggggc
      961 tgggtatggg atgatgatca tattcgcgat ttgctggtca gcattgccag gtcttttccg
     1021 gttggtgcaa taatgctgct agagacaggc ggtccggtgc gttttcagac acgccccgta
     1081 gaagggctgg aaaaaatagt tccgacagag catgctccgg aaaaacttat tctggatggt
     1141 cagcagcgtt tgacgacact tacacaggca ctggcgcttg ccgcccctgt tgaaacgcgc
     1201 accgggcagg ggaaaaagat taaacgacac tactatttta atatccgcca ggcgctgagc
     1261 atgcctcagg atttaagctc tgctgtgatt ggtgttgatg aaaatcgcca gatacgttca
     1321 aacttcggtc gagatattga gctggattta tccacccgcg aaaaagagtg tcaggagttg
     1381 tattttccct gtgatcagac aatgaattca gatgaatggg aagaaacatt gcatcgggtt
     1441 gcaccggagt attttgaact ttatatgggg tttcgacggt cggtattaaa atctttccgt
     1501 gagtatcaga taccggtcat tcaattgcat aaagagacca ccaaggaagc ggtatgcctg
     1561 gtttttgaaa aagtgaacac aggcggagtg cagctttctg ttttcgaatt gatcacagcg
     1621 agctatgccg ccgaagggta taaccttcgt gatgactggt acggttctga tatccgtaaa
     1681 gttgattcca gacgagaggg gcttgttgcc gagcccctgc tctcagagat tcaggcgaca
     1741 gaatttctac agggggtaac gctactgcac acttttgaac gcaaaaaatc tgacagcagc
     1801 agcgggagaa ccgggaaaga ggttcgtcct gtcagtgcca aacgaggtga tgtgcttgag
     1861 ctgcccttga atgcctggct gacatgggcg gataaattgg aggcaggttt tcgtctggca
     1921 gcaagatttc ttcgtaaaga atcgttttat tcgtcgcggg agttgcccta cagtacacaa
     1981 attgttcctt tagccgcagt acttactcat ttgggtgaaa agtggcttga acctcgtatt
     2041 tatgacaaac tgacacgctg gttctggtgt ggcgttttgg gcgaacttta tggaggcgcg
     2101 gtggaaactc gtatcgctaa tgactttgaa cagttgcttg agtggattaa gaaggatgat
     2161 caggagccac gaacagttta cgacgccagt tttcagcccg gtcgctttga cacacttcgt
     2221 tcccgtttaa gtgcggctta taaagggatt aatgtgctgg tgttgcggga aggtgcacgg
     2281 gactggttat ggaaagccag tattcatgaa ttggacgtgg acgaggtaaa attggatatc
     2341 catcacattt ttccaagaaa ttggtgcagg aagcgcggta ttcctatggg atcctacgat
     2401 tccatactca acaaaacccc tctctcctat aaggcgaatc gtaaaattgg aggcgaggcg
     2461 ccctctgcat atcttccaaa aattcagtca gaaaaacacc ttcaattgag tgatcagcaa
     2521 atggacgcac ttttagaaag ccacgccctg aatcctgctc tactgcgtgc ggatgactac
     2581 gaagcattca tcgctgatcg tcggagaagg ctgagcgcac tggtttcaaa agcaatgggt
     2641 aagccggtaa gtcaaatcgg tgaaagcgaa gtttacgagg aagccgaata agtcagagca
     2701 gtggaagcct gataaggagt tttattgtga aacattctga gcctccaacg gaaggtgaaa
     2761 tcatcttgta ccaaacggcg gattcaaggc tgaaatgcaa aattgtcgta tgcggattca
     2821 aggctgaaat gctgaaatgc aaaattggcc tgaaatgcaa aattgcgttc aatagtgagt
     2881 tcgcgaagtg atataatgaa cctcaaggca taacatgagc agaccagcag atactttctc
     2941 acaacttttt tttgaagttt ttgagtctct ccccaggcag ggaccgggca gccggaattc
     3001 agcggcaaag gcgctttcct tctgccgtga actgccgtcc gggccgctca ttcttgatct
     3061 gggctgcggc agcggaagcc agacctttca tcttgcggaa ctgacttccg gctctgttgt
     3121 ggcgctggac agtcacgccc caagtattga taaactccgt gcagcagcag cgtcgcgggg
     3181 atatgacagc cggatccggg cggtggtggg ggatatgtca aagcccgatc ttccgcggga
     3241 tagttttgat ctgatctggt cggaaggtgc cttttataat atcggtatcg cctgtgcgct
     3301 ggatgtttgc aggcccttgc ttcgtgccgg cggttacctt gcctttacgg atgcggtctg
     3361 gcgcaaagaa aatccgcctg catcggtcaa ggctgttttt gatcaggact atcccggcat
     3421 gggacgggcg gaagagattg tcgcattgat acaaaaagca ggttttgcgc tgatcgggca
     3481 gttaatgctt ccggacgagg actggtggga cgatttctat acacccatgg aacttcgtat
     3541 cgatgaactg cgccaaaaat atagcggaga caaggaggcg cttgccgcac ttgccgcact
     3601 tgccgaagaa cccgaaatgc accgccgcca tgcagcctgt tacggctatt ctttttttat
     3661 tgcccgcctt gcgaagtagt cgcatgtgcc tgccgcctgc tgcaatgccg ccttcctgac
     3721 agatcttcat gcactctcat ttatgcctca gcctgtttca gtgaaaaata acccttgaca
     3781 aagttcctta ttgtggtatg ataatgaaca aggaacagca gcatctgctg ataagaaagg
     3841 gttctgccat ggcacgtctg tcacagattg atgaggtgat gtttcctgtt gaatcgtgtc
     3901 ctgtctttgc atgtgtgcct actgaccagg gggaacggca catgcctgca tcagatacaa
     3961 aagcgattgt gaatatgaac acgcacaagg tggtgggggt tgtgagcaaa ggttaccgtc
     4021 tggtgaccaa tcaccaggcg ctgcagtggg catatcagtg ctgcgagacg gtctttcctg
     4081 agacaaagcc aaatgaatgg ctggtgaaaa atgcggactc tccgaagacg ggttctcatt
     4141 gctttattga tcttgtgcat catacggcgc tgctcgattt ttgttttgtt cctgcatcga
     4201 atcgtcctga cacctacggc ccttttatcc ggctgaccaa cagttacaac ggcagtcgcg
     4261 ctcttacttt tcatatcggc tttttcagaa aagtatgcag caacggtctg attcttccga
     4321 aagcggtagt cagtttccgg ttcaatcatc agcgccggga aataggggag taccttaatt
     4381 ttgcagtggc aaacaatcag gtggcgaacc tgaagaaagc gctcaccatc tatctgggtg
     4441 cgcttcagga ttgtaaagtg ccgcataatg agctggtacg gcttttctgc ctcattcttc
     4501 tcatacgcca gccggctgag gtaaagcccg acgatcccat gcgtattgaa tgggaatatg
     4561 tttacagtca tatcaccgga ttgtgccgca aatatcagaa tgatttcgga aatacggcat
     4621 atgcggtatt caacgccgcc acggagtttg cgtcgcatcc gattcagagc cgctgtgtgc
     4681 gccgtgaacg ccatagctat cagcggcttg ccggaacctg gctggcacaa tttcaggaag
     4741 aatgtcagca accgggtttc agcatccccg attatattga aaaaatgacg cgaaaaaaga
     4801 agagcagcac aagagaaccg cctttgttcc aaaacatcga catgccagtc ttaccgggct
     4861 tcctgtaaaa acggcacctg accgccccgg gtcgatgccc gcacagcagc gtcagggatg
     4921 aatccggaat ccgggagccc agccaagttc cgtttgtgct ttactgattg aaacagtgcc
     4981 gtcatcttcg gcgatattgt atacgcccgc ctgccctttt gtcagggcgc gccttgccgc
     5041 atccgctgca tcgtccacgt ggagagggcc gtcttttgcc gcctcgtcaa agcccgtgcc
     5101 cggcccgtag aatttaccgt agcgcaaaac aatccccggg aaaggcgcat tcaatacctg
     5161 ctcttcgaga ctgatcaccg cccggacggt gccggtaaag gacggatccg ccgcgcccag
     5221 gggtgattct tccgtgtagg gtttcggacc cggcgcataa acaaagccga ggctttgcgc
     5281 caccatacgt tccgcgccgg cggcggtggc ggcggcaatg agatttcggg tgccttcttc
     5341 acggattcgt gcgttgcgga tcagcgcatc cgccattttc gccggatcca gcgcaggcgg
     5401 cagatcggtg agctggtgca ccaccactgc aggctccgac tttttcacga gctcggtcag
     5461 aagcgcttcg tcaaatacat caacaacagc aggggttacg cccaggccac ggagcagctg
     5521 cgctttttcg tgcgaccggg tggtgccggt aacagaccat ccgtccgcaa caagcagttt
     5581 gcagagtctc cgcccgacag cgccgctggc accggcgata aagacattgc ctttcatagt
     5641 gtgtttcctt taatggttgt cggctgcctt acccagcgct ttttgaattg cgccctgcag
     5701 gtctttttcc gccggcgcga tcagttcgat cagccgctga gtgtcgtcga tcaatgccac
     5761 ggtggggatc tcctgaatac cgaagcgccg attcagttca ccgccctcat ccagcgccag
     5821 aggaatggtt acctgatagt ttttcccgta ttccgccagg tcttcttttg tagtccaggg
     5881 gccgcccgca ataccgatcc agtccacctc gggcacagtg cttttcatgg tttctatggc
     5941 ttctctcact ctggtgcagg cctgagaagt atcggggcgg ctgctttcca ggtaccattc
     6001 gcaccaggtg gaaaaaaact gcaccgccag cagatgcccg gattccgctt tgagggggag
     6061 actgccgttc tgcagcgtgg caacggacag atccggcact ttctctccga cctgaatcac
     6121 cttttcatgt tccagccgct ccggcgcaag ctctgcggga tccgcttctt cctgcagcac
     6181 ctgctttaac gcctcagcca gcgcctcgtt atcggcgtga ccgatatatt tgaagcgtcc
     6241 gtcttttccg ataagcacat gctgcggggt cacggtcaga tgaaagcctt tcgcgagtga
     6301 accgtcgtcc atgacaatgg gcaaagtcag ccccagtttt tcgcggaagg cacggactga
     6361 tgcctcgtca tcgctgtatc cgatatcaat ggcggcgatt tgtatccggt cgcccagtgt
     6421 ccggtagatt tcttcgaaag cgggcatctg ctgccggcac ggaatgcacc aggtcgccca
     6481 gaatttaaga taaacgggtt tcctgccgta gatggcggag agatcaatct gctgaccgtc
     6541 tatggttttc atgacagccg ccggcgctgc tgtcccgatg acatccggcg caattcccag
     6601 tgcaagcgcc tcgcctgccc gggtcgtttc cgggtcggga gatgcattgt ccagattcat
     6661 tccgtcaaaa cgggttgccg tcatcgcacc attttcgtca gccggaacgg gaaccgccat
     6721 cgaaagaata atccctgcaa tgatgatgca gagcctcatc ttgtctgtat gcatgctgtt
     6781 tttcctttat gcgctttggc agctttttgc cagcctcccc ccgatgcgct ccccgtacag
     6841 tgaacactaa cattgcgggg cgctctttca tttcctcact gctacggatt atcataaaga
     6901 gaggaaccac gaagggacac gaatagacac gaattaatat ttaagaaaga agtatttcag
     6961 attgtgggct gtaccatgga agtgttgaat acgctggggc acggactgct ggaaaagcca
     7021 taagaaaatg cgctggctgt tgaggcttag gaagatttat gagtttcaga ttcagcgcat
     7081 ggttttgcga gacgtttaaa aaggatgaac aatgattacc ggtgacgaga acgatttttc
     7141 ggaaattgta atttaccaaa gtgaagacgg cgagatcagt ctggatgttg ttctggagga
     7201 tgaaacggtc tggttaagcc aggagcaaat ggggaagctt tttggaaaag ataaaaggac
     7261 gatatctgag catatccagt cgatattttc cgaaggagaa ctggacgaat caggggtggt
     7321 ccggaatttc cggacagcca ctcggcacgg agcaatgcca gacagaaagc agacgcattc
     7381 ggtgcgattt tataaccttg atgtaattat ttcagttggt tatcgggtta gatctttgca
     7441 aggtacccgt tttcgaattt gggcgactcg gcggttgcgg gaatacatcg ttaaaggctt
     7501 tgctttgaat gatgatcgtt ttaagagcgg ccattcgatg aactactttg aacagcttca
     7561 ggaacgaatc cgagccattc gtctctcaga gcgttttttt tatcagaaaa taaaagatat
     7621 ttatacgaca agtattgact atgacccaaa ggatgaaaag acacttactt ttttcaagac
     7681 ggtgcagaat aaattgcttt gggcaattag tgaacagacg gcggctgaac ttgtttaccg
     7741 acggaccaat gcagcattgc cacttatggg gatgcaatcg tttgataaga acacttcagt
     7801 cgggataaca aagaaagatg taagtattgc gaaaaattat cttgacgaag aagagatgaa
     7861 acttcttgga cttttagtag agcaatacct tgcatttgcg gagactatgg cccaacaaca
     7921 ccgaccgatg actatgagcg actggattga tcgtcttgac gcaataataa gtttaaatgg
     7981 acgagaactg ttaacgcatg ctggcagaat cagccatgag atggctgttg aaaaatctag
     8041 tatagaatac aaaaaatatc aagaattgaa aagaacagtc gagcgtgaag agagtataaa
     8101 agaattggag tctgacttaa atcgtttgga atttcacaaa aagcctgatg atgcgtagcc
     8161 gctgcgatac gaaatatatc gcccgcatat gattcttatt caaagtagag tggtagtccg
     8221 gaaattccgg acagccactt ttcttgattt tcttagttat aaaataagag attgtgaaat
     8281 atgaaagttt gcagccactg gaagctggcc ctgctttgcc tccgttacac gagaggcttg
     8341 cttcccgtat cgaggtgggc gatagtatgt tcccagcctt ttcttctgaa gacaagaaag
     8401 ctgaggagga ggcggaggat atggctgagg aggatggcgg accagatcac ttccggggtg
     8461 agtctgttaa ggctgaggaa gatctggcag aagccgagga gcgttacgat ctgagtgaca
     8521 aaagcgatga gcatgggggg gcgtgttgct cccgcgccct gcaggccgcc tgtgagcgcc
     8581 tgagcgatgc cgagtacaat gcctgacacg ctgagatagc ggagcagcgt cacagcatag
     8641 tcatagacga gaccgtcggt cacattaaaa agagagagca gcgtacccgg aatgctgata
     8701 tacagaatac ccaccagcgc cgcccagaat ccggcaagcg ctccggcgat aagcacgcct
     8761 tccttgcccc gtttgggatt tcccgcaccg atgttctgcc cgatcatagt gccggatgcg
     8821 ccgcgcagtc cgaaactgat ccaggacaca agcgaaaaaa gctgggtgta acagatgata
     8881 tacgccgcct gtgccgccgc gctgtattcg agcaggctga tataccacag gagcatgacg
     8941 ccgccgatat tgaggagtac gccctgcaag ccggaaggga tgccgatccg catcatggta
     9001 atcagaagcc gcatatcggg catgagccgg tgccgctcgg gcggctgaat aatggtgcgg
     9061 cgtttataga taagccggat gcccagcagt ccgctcacca tcggggcgag taccgttccg
     9121 aaagcggcgc cgcgtatgcc cagaggcgtg aaaatgccga gccctgtaat gagcaccgtg
     9181 ctgatgacaa tgttcaatac cgtggtgagc accccgagtt tgagggggag cttcgggtcg
     9241 cccgacgcgt gaaatgcgcc tgtgagcaga aacatcagaa agagcggagt tccgcaaaca
     9301 aagaggatcc gcagataggg gaggcaatgc aaagcgacct caggcggcac attcatcagc
     9361 cgcagcagtc cgggtgccaa aaggtagccg atcggtccga agataaaagt gagggcaaca
     9421 aaggacgcca tgaaagcggt ataaaagacc cggctcagcc gttcgcggtc ctgtcgccct
     9481 gcattgcgtg aaatcagcac attcatcccg tggaatacag aagtgataaa cacgacgaga
     9541 acaagaaaaa cctgccatgc cacgccgatg gcggcattgg cggcgttatt gtccgatacg
     9601 atgaagtgcc ccaccagaat atgatcgaca atgccatgcg tgccattgac gagattgagg
     9661 cagaccagcg gccatgcgag tttccagaca gaccggagcc tgctgccgct gattatttcc
     9721 aggtcaaaag atttcaaaag tgcgagggtc tttccggttg gagggggatc gatgtatatg
     9781 caataagcct gctgccgtct gtctgagacc tgtccgggtg cggctgtccg caactgaaaa
     9841 aatgtcccgc tctgccttac tatgataaaa tgcaacgata gcttgaatca atgcaacccg
     9901 aagaaagtgg atcaatgaaa gttggactga taggatttgc gcgttccgga aaaaccaccg
     9961 ttttcaatgc gatcaccggt gcccgggcgg aagtgggtgc ctacggaagc cgtgatgcga
    10021 atatagccgt gatcaaagtg cccgatgcgc gggtggatgc tcttgcggaa atctataaac
    10081 caaaaaagaa aacttatgcg gaaattgagt ttgtcgatat tgcgcctgcc gaagggagcg
    10141 atcaggatcg tgcgctggat tccgcagcac tgacccttct gaaaaatgcc gatgccctcg
    10201 ctcatgttgt ccgcgcgttc aaaaacgata atgttatgca tcccagagat accgtgaatc
    10261 ctgtccgtga cgtgcagtct ctggaagaag aactgcagct catcgacctg attgttctgg
    10321 aaaaacgtgt ggagcgtctt gaaaaagagc accgcaaaga tcaggaatat gagctgatgg
    10381 ttcggtgtaa agcccatctt gaatcgggca agccgctgcg cacgcttgat ttcagtgccc
    10441 aggatgcgct gttgctgcgg agctattcac tgctgtcgca gaagcccatg atgcttttgt
    10501 gtaacagcgg cgatgacggg ttgggcgatg cggatccggg cgggctttcg gaaatagcct
    10561 ccgcgctcgg tcttgatctg atcagttttt gcggtgcact ggagatggaa gtcaatggac
    10621 ttcctgaaga ggagcgggct gcttttcgtg aagagctggg catgggcgag gaatcccgca
    10681 cccgcttcat tcagttcgct tacagcatgc tcgggctgat cagttttctt acggcagggg
    10741 agcccgaagt gcgtgcctgg accattcgca agggtgatca tgccgtggat gccgccggtg
    10801 tgattcattc ggacatcaaa cgcggtttta tccgtgcaga aacagttgcc tatgcggatt
    10861 tcatggaagc gggctccatg gcaaaggcaa aggaaaaagg caaagtacgg ctggaaggca
    10921 aggattatat cgtacaggac ggcgatatga tgcttttccg cttcaatgtc tagcgtctga
    10981 aagagaggtg cggtgccgtt cctccgatct gctcccaccg gtcgcagttc cggtcgaaag
    11041 gtgttccgtt ttcctctttc atcacccagc agcctgcctt cgcatccaga tcgtattcca
    11101 gtgtggtgcc gccgggtcgt gcaaaccgcc cgctcagcgc agcttcctcc gggttggctt
    11161 cctgtatcca ggcaaggagc gcatccgctg aaaggtctgc ttcatgaaag agtgccagca
    11221 ggcagacctg atcctgattt tcataaaggg caatcacaag gggctgtgtt atcggcgccc
    11281 gaaaaatgcg ccagcctccg gcagcggctt ccggcgtcca gccttccggc acatgggcag
    11341 ttcccctgat acaggcgaaa tctgtataaa cgcctgtggc gttccagctt ctccgttttc
    11401 ctgctccatt cagatggata cagccgtcca gatagcgctg tccgtcttcc agcatcagcg
    11461 tgatcggcat ggcaacgaca agcgagcgca gtccgcggtt gaatccgcct gctgtgagaa
    11521 gccatcccgg tccggcactg taaatttccg ggcagccgga agcaccgtgg gaaaagcgga
    11581 cgaaataagg ttcttttttt tccagtgccc attgccgcag cgcttcaggc aggcggtagg
    11641 gcaggaggga agcaatgagg ctccagtcgc tgtagttgcg tgcccaggga atcacccggg
    11701 gatcatccgg ctcgacccct gtccaggcat agaagaaagc agtatgcgga tcgctgtcga
    11761 gttcctgtgt atcggcacgt cggtactggc gccggaaggg gggaaagcgt cgcagctgca
    11821 ggctgccgag tgcataggta agattctgcc ggtcgaggat gagccgtgcc ttctgccgga
    11881 cagccggtga tgcggcaaat ccctccagat tcagcagggc atggatggtg tagcctgaat
    11941 agggacgtga attgaactcg taaatgcctt ccgtaaggac agcctccaga aaaccgagga
    12001 gccagctttc cagtccgttc cgggcattat cccagcgcgg gtccgcctgt tccgcttcgg
    12061 ttccgttcag gtgcagccac tgatttttca ggtaccggct gctttccgca agaaggatat
    12121 ggttttccgt ttccgtgatc agtctgcagg tgcccggtac tgtggttttg ggttttcctc
    12181 ccgactccgt caggagcacg tccagcagat gtgctttcgt ttcgggataa agccgttccg
    12241 gctgatcacc gaaaagatag agcaaggtcg tccaggctgc aagagaaaaa tcataatccc
    12301 ctttgcgcaa aagccaggaa gagcccacgc ccgcatgagg ccgccgctcc cgcagcagcg
    12361 cattgatccg ctctgtgtcc ttgcctgagg caagccaggc aagaaagttt tttggagaaa
    12421 atgtcctttc ggttgcctct tcaggccgca cctgggctgt gacgatgtcc agaagctgca
    12481 gccctacagg cggaaggggg gaagggggca ttgaaccatt catttcagta tcaggttcag
    12541 gcataatctc gcccggttcc atggtatgca tgcggaatct gcccgatccg ccaaagatgt
    12601 ggatgcccgg caggatcagc gccaccggaa tggcgcccgg cacggtcagc agcactgtaa
    12661 taaagaggag tgttcttttc ggaatttttc ttgtcttcac agacggattc cttggtttca
    12721 gaaggttaga gaaaacgggc ttctttcgga ttgatccact gaaagaggct gcagttcact
    12781 ttacggagca gcagatccag tctgaccagt ggcaggccca gcacattctg gtagcacccg
    12841 tcgtagcccg ctaccagcag gctgcccggc ccgtccacgg tgtaggcgcc cgcccgatcc
    12901 agaggccgca cgatctccac aaaacagtcg atttccgctt cgctcagttc tctgaagcgt
    12961 acgcctgtcg tttccgaatc ggtaatgagc tgctccgatg cccgatgata cacagcaagt
    13021 ccggtcacca cctgatgcgt gttgcctgaa aggcggcgca gcatagagcg cgcctcgtcc
    13081 aggtcggcgg gcttggacaa tactgcgccg ttgagaaaaa cgagggtgtc ggcgctgata
    13141 atgaggtcgt ggcagggatg ggcttcggct gccgctctgc atttacgctc cgcattgccg
    13201 atgacgatgg attcagggga ttcaccctcg tcgatttctt cagcctgtgc cgcccagatg
    13261 tcgaaagaag caccgagcgc cttcagcagt gctttgcgcc gcggcgatgc ggaagcaagc
    13321 agcaccgata gtccatttct tgttttcata gaatacctgt gagcaccaga gggtgtcatt
    13381 gatttaatct gagatttttg gcataatttt atacagacat caaaccagcc cggtcgggaa
    13441 ggtcccgggt caggctgtac tgttccagac aacttcaagg agtttcaaag catgaaacat
    13501 gtaaaacaag tgacccagcc ccgcaaagcc atgtccacga ctgacatcct tacgctggtc
    13561 ggctccattt tgagtggtgt cggcaccatc ctgatcactg tggcgcagtc tgtcggcggg
    13621 agcaaatccg tcggctgagt ctttttcgcc ctgcgacatt tttcggatcc gcccggcatg
    13681 cggtatccgt ttctgaaaag ctgccgtcct gctgtaaaaa aaacagaggg gcggtggctt
    13741 tttcatatct gctcctgtac aggggggcag aactcggggc tcaggggcgt gtgaagtaaa
    13801 acaatgcacc cgcaagactc agcccgacca gcacaatggc tgcccgcagt gtccagagga
    13861 tggcagggcg catggcagct ccggcatctt ccggcaccgc cgccgcttca gcagccgccg
    13921 gagcatccga aacaggccgc agtctttttt ccagattctc cgccatggat tttgcccgct
    13981 ctgccagttg aatatccttt tctttgaccg gactccatcc tgatgcggtt tccaaaatgg
    14041 tgtaacccat gcttttccag gtctttgcca gacgttcccg ccgcatggca ggcgcttcat
    14101 agctgacggc ggcacgtctc atggaagtgc gcaccacttc atgagtccgc caggggatat
    14161 cgggcacaaa ttccacttcc tgagtgggct tgatttcctg attacggtcg gtgacccatt
    14221 gcccgccaac ctgcatggct gcataaaacg gagcagctcc gctcagggtt actgaaaagg
    14281 tcggaatctc cgctggtacc gttcctgtga agagcgcatc ggtctggctt actgcggaaa
    14341 ggacaaacaa taacatcatc atacaatatg aatccctggt gtttttccat cggatgcacc
    14401 actgccttta gtatacccga aattgaacgg aatgttcttc aacaggtatc atgttattga
    14461 aataaaaagt gcaaacgctc aggcggagca gttttaccgg cgtgggacgg gggcgggttt
    14521 cccggtttcc gggtacgctg aagcaaaaca ggaaagaaca cagcagaaag gcatgtgcca
    14581 tgaaaaaaaa tatttatcag aagatctggg aagcccatct ggtgtgcgag ccggaagggg
    14641 aggacgccat cctctacatt gaccggcatt atatccatga ggtgacatcg cctcaggcat
    14701 ttgaagggct gagactgtcc gaccgcaaag tccgccggcc cgacctgacc tatgcgacga
    14761 tggatcacaa tatccccacc acagaccggt cgcttcctat tgccgacatt ctgagtcgcg
    14821 cccaggtgga aacgctgaag aaaaactgcg cccagttcaa tattgaatgc ttcgatatgt
    14881 atgatccgcg caacggtatt gtccatgtgg tcggaccgga actgggcctg acccagcccg
    14941 gtatgaccat tgtctgcggc gattcccata cgtccaccca tggcgctttc ggcgcactgg
    15001 cacacggtat tggcacaagc gaagtggagc atgtgcttgc cacgcagacc cttcgccaga
    15061 aggcgtcgaa aaccatggaa gtgcgtatca acggaacctt gccgcccggc attacctcca
    15121 aagacattgt ccttgccatc atcggcaaaa taggtacttc cggcggcacc ggctatgtga
    15181 ttgaatatac gggtgaggca atccgcaatc tcagcatgga agcgcgtatg accatctgca
    15241 atatgagcat tgaagcaggg gcgcgcgccg gcctgatctc cccggatgac accacgatcg
    15301 ctttcctgaa aggtcgggcg atgctcccga aagaccgcag ttttgaagcg cttgcggaag
    15361 aatggaagca gtgggcgtcc gatcccggat gcagctatga cacgctggta gaactgaatg
    15421 cggaagatat tgagccgcag gtatcctggg gcacgtcgcc ggaaatggtg gggggcatct
    15481 ccggcacaac gcctgtgctg gcggatgttg ccgatgtgaa caaaaggcat gcctacggaa
    15541 aagcactcga atatatggga ctggaagagg gcatgcccct tcagtccatc gccattgaca
    15601 cggttttcat cgggtcgtgt accaacggac gcattgaaga tctgcgcgaa gcggctaaag
    15661 tgctgcgcgg ttataaaaag gcagactccg tacagcgggc gctgattgtg cccggctcgc
    15721 aggcggtaaa gcggcaggcg gaagaagaag gacttgaccg ggttttccgg gacgccggct
    15781 ttgaatggcg cgagtccggc tgttccatgt gccttgccat gaatgatgat aaactggcgc
    15841 cgggcgaacg ctgcgcttcc acctccaacc ggaatttcga gggtcgccag ggcaagggcg
    15901 gcagaactca tcttgtcagt cccgccatgg cggcagcggc tgccgtcaaa ggcgtcttta
    15961 ccgatatacg caactggaaa tatcaggatt aacccgaaca tgcccggaac cgtttttccg
    16021 gtaaggaaac catgcggatc ccaggaagga atacatcatg aaccctttca ccattcataa
    16081 gggacttgtg gcgcctctcg atgccatgca cgtcgacacc gatcagatca tccccaagca
    16141 gttcctcaaa cggatcgagc ggacgggcta cgaggatgtt ctctttttcg actggcgtta
    16201 tcttgacgac ggaaaaacgc ccaatcccga gttcgagatg aacgcacccc gctatcaggg
    16261 tgccagcatc ctgcttgccc gggacaactt cggctgcggc tcttccagag agcatgcccc
    16321 ctgggcgctc ttcgattacg gaatccgctc cattattgcg ccttctttcg cagatatctt
    16381 tttcaacaac tgcttcaata acggcatgct gcccatccgc ctgtccgcag atctggtgga
    16441 ggagctgttt cagcagaccc gtgccgccga agggtattcc ctcgaaatta atctgccggg
    16501 gcagaaaatt atccgccccg acggctcggc gatttccttt gaaattacgc ccttcctcaa
    16561 gcagcgcctg ctcaatggat gggaccagat cgggctgacc ctgcgcta
//
LOCUS       DAOPWB010000073        16356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3608_length_16356_cov_5.992823, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000073 DAOPWB010000000
VERSION     DAOPWB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3608_length_16356_cov_5.992823"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="PKN92_08050"
     CDS             complement(<1..1245)
                     /locus_tag="PKN92_08050"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNZ48784.1"
                     /translation="MQDTLLRLLSTGGLWVMLGAAWALSDNRRKIQWRLLFWGIGLQA
                     LLGLLVLRTGLGTGLFNGVRRVFDLLTEASTEGAEFVFGNLSRFFMLERVLVPGPDGV
                     MAAQEPFAINAIFAFNVLPVIIFVAGIAAILQHLGIIQAVVRACAWLMRRTLKTSGAE
                     TFATALLVFTGIESMSAVGAYLQRMTRSELFTVMVAFLSTIAASVMVAYASFGAEPGH
                     LLAASLMSAPAAILISKFMIPETEKPETLGDVSVSIPVESENIFDAAVGGASLGLNMA
                     LHVAAMLIVFVGLIHLLDQASLALTSSPATQWLGWFFYPFAFAMGVPFNDLPAFSQLL
                     ATKTIFNEFIAYQQLQTHIIDGSLSPRSVVIATYALCGFANPGSLGILIGGISAIVPA
                     RRAEVARLGLRALAGGTLAAFMT"
     gene            complement(1272..1763)
                     /locus_tag="PKN92_08055"
     CDS             complement(1272..1763)
                     /locus_tag="PKN92_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNZ48785.1"
                     /translation="MASDELPVSPYTTILCCTDFSENAARAFTFALDAAVRRPGSVLH
                     LLHVIPEPEAQFWKTYVYNVEGVDEKARSAIDQKIERDYRPHVPDSVSLEVVMRIGKD
                     YLQILEYAGEIRADLIVLGRHGHSALENVLFGNVTEKVVRKAACPVLVVPRASAKTGD
                     RAG"
     gene            complement(1786..3087)
                     /locus_tag="PKN92_08060"
     CDS             complement(1786..3087)
                     /locus_tag="PKN92_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNZ48786.1"
                     /translation="MTPIQTGILGGIALVFFLISSMPVAFAMALVGIVGFAMLTTSTA
                     AMSMASAELYETFSSYSLTVIPLFVFMGQVAFHAGISKSLFNAAYKWLGALPGGLAMA
                     TVGACTAFGAICGSGPATAATMASVAIPEMRRYNYSMELASGAVAAGGSLGMLIPPSV
                     VFIVYAILTESSIGKLFIAGVLPGLMIAALFCLTIYINCRRRPEMGPVGPRASLGEKI
                     RSLAGVAETLILFIVVMGGMFLGFFTPTEAAAVGAAGSLIIALVKRKLSIKILIRSLL
                     ETARTSSMVMMIVAGAVIFGRFLAITRIPYELANWLSNLPLPGAMIVFLVIIFYLLAG
                     CFVDALALVLLTIPIFYPVIVELGYDPIWFGVVIVVVTQMGVISPPVGVNVYVVGGIE
                     RDIPLSTIFKGSMPFLCTLIVAAVLLTLFPQISLFLPGLVK"
     gene            complement(3084..3614)
                     /locus_tag="PKN92_08065"
     CDS             complement(3084..3614)
                     /locus_tag="PKN92_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNZ48787.1"
                     /translation="MNIFHHSCLKSLQAAGRFFWQSMRFLVYALAVLSGLCILAMMCI
                     TCVDILFRLGGYPLKGCYDLVRIFGALTIAGALPYTTAVKGHVAIEYFFLKLHRRGRI
                     VIDSLSRLVIMALFGTFAWQCLLYGNALKRSGEVSLTLGVPLFWLSYIIAFSLGLTML
                     VTLHNLLHPGREMIKP"
     gene            complement(3622..4677)
                     /locus_tag="PKN92_08070"
     CDS             complement(3622..4677)
                     /locus_tag="PKN92_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNZ48788.1"
                     /translation="MFRKIAVLTCVVSLFSVGSLLVMAGCGSPAPSDGSAKKAKPIVL
                     SYSIFFPPTHAQCLEAEAWAKEVEARTDGRVKINIYPGGSLSQAPQCYEGVVSGISDL
                     GMSCFSYTRGRFPLLEGLDLPVGYPDGKTATRLATDLAKKYAPAETSDTHLLYVHAHG
                     PGILASKKVVASLEDMAGLKVRGTGLSAKIIERLGGSPVGMSQPETYEALQKGVVEAT
                     LCPVETLKGWKQGEVISSITDSSVIGYTTAMFVTMNKGKWDSLPADIQNIITAVSEEW
                     VDRHGAAWDKADEEGLAFIRELGRTETALSAEEQARWREAVQPILDEYTAACTAKNLP
                     GAEFLADLQKGIAAASR"
     gene            4947..6836
                     /gene="dnaK"
                     /locus_tag="PKN92_08075"
                     /pseudo
     CDS             4947..6836
                     /gene="dnaK"
                     /locus_tag="PKN92_08075"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            complement(6946..8184)
                     /locus_tag="PKN92_08080"
     CDS             complement(6946..8184)
                     /locus_tag="PKN92_08080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ48789.1"
                     /translation="MRIAVVDLLFSWPPHGGADVDVYYVMQELMRLGYDTHLFVTAEE
                     GSWERGDIRDGALPFPVTLLELGEQGLTEAFLHNRLYEAVVGFSPDIVFLTQGYFLKT
                     ALIEALKDYPIISRCYAHELSCHKDILRFREGTPCPHCYVESPDICRRCALASQRESL
                     FAGTAHAWTREYVESRAWTKDYYSRFKKAMAQCSKVIVTTEHMRAPVELLTKEIAVIP
                     NGVDIRRFLPSPERAGRPVPVLFVPGRIEDPAKGFSLFLEAAEKVSGSGIAFEVRATL
                     PEGYEGPPWLKAVGKLDYDSMPQAYRNTDICVVPSLWEEPFGLVALEAMASGIAVCAS
                     RTGGLQDIVVHGETGFLFERGNSRALAACLERLLEDRHLCAAMGARGRQRAEDVYDWE
                     HIVSAYYPPLLDEISRMKQR"
     gene            8379..9350
                     /locus_tag="PKN92_08085"
     CDS             8379..9350
                     /locus_tag="PKN92_08085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ48790.1"
                     /translation="MIYEFSALLLNPLIGGWKVAEIPELEYSVIADRYVVREKLGRGG
                     MGSVYLVQDKQTGQMVALKTLHTKYANSKHAIARFIREVNTSRKLNHPGIVKVYEASK
                     WNNTLFYTMEYIRGKSLRRWLQERHNLEFNSVVRVLCLVADALQHAHQITIHRDLSPE
                     NIMVLPDGSIRLLDFGLAKLDDQFKGLTVVGANLGKIHYMAPEQQLNPAGVDYRADIF
                     SLGVMFYELLCGRSPQSGYKVTEIRPELPVECNAFVEKATHPDPNLRFQSSREFRDAL
                     LNLYRIQEARTKKTVKKPEGLFGKISAFFTNLFSRPQKSGSQRKRGR"
     gene            9361..10038
                     /locus_tag="PKN92_08090"
     CDS             9361..10038
                     /locus_tag="PKN92_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNZ48791.1"
                     /translation="MGKTKAIASATILLSLVLFQTDGCAENDEITDHYRRMMVYHEYM
                     DGNVPDGAVVFIGDSITQGLCVCAVTPLAVNYGIGSDTTYGVLQRLPKYKSLEHCAAI
                     VFAIGVNDLSRRSDDEIISNWKLIMQSIPEDIPAIFSAMHPLDAETKNISEESNQRIV
                     RLNEALKNLCAENNQYFASAFTKLVNEKGNLDPAFHTGDGVHLNTAGYQVWIAVLQTA
                     LQSIPQE"
     gene            10174..10446
                     /locus_tag="PKN92_08095"
     CDS             10174..10446
                     /locus_tag="PKN92_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HNZ48792.1"
                     /translation="MSDDLTARFERASKEVTELSEAPDSAAQLELYSLYKQGSVGDCT
                     GERPGMLEFIKRAKYDAWKERAGMSQDEAKEKYIALVEQLKEADKK"
     gene            10571..11605
                     /locus_tag="PKN92_08100"
     CDS             10571..11605
                     /locus_tag="PKN92_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48793.1"
                     /translation="MNVDIRRFLCGLPVFLLLCMFPVSAFSADAGADADPDNLSLFNS
                     LSAAQVFEAARASVKETFSGIPESFESGDVLVVLRKQEKVTVRLYGVDCPEEGQPFFE
                     EAKTGGHSFLGGEAAEISILSVDNKDLPVVLILNKEKQSLSHYLAAKGLGWWDEKNAP
                     KDALLRKLSAGAVLKGQGIFSQATPLAPWDYRKSKEIEDFTYTLETVAEPVPDKKTAR
                     SDSESSEKSLSKKGTMVESAPRTAPVQLKAPAEKIDAGALMMRHQPRISRDESGRPQG
                     LTATDISAIPYAAQLGFQNGDIISRVNGIAIESEAQIMGLIPQFQNVKQFQVEVIRNG
                     RSITIPISIP"
     gene            11767..13593
                     /gene="thrS"
                     /locus_tag="PKN92_08105"
     CDS             11767..13593
                     /gene="thrS"
                     /locus_tag="PKN92_08105"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNZ48794.1"
                     /translation="MNNCSVSVADKKETALYRLRHSLSHLLAAAVLEIRPDAKLAFGP
                     PVENGFYYDFDFGGTPVGEADLADIETRMNALIKKRIPFVRTEMPFEAAVKHLEAGGE
                     VYKIEYARELLDKGLAVNGSLSFYTMGDFIDMCEGPHLEHTGEVPKKSFKLDRISGSY
                     WRGDESRPMLTRIYGLAFESADALKEYLEMRKLAMERDHRKLGDELDLFIQDSEVGVG
                     LPLWMPNGTIIRDELEKWARETEFLGGYQRVSTPSVTRKELYLRSGHVPYYQDSMYPS
                     MPVDEHDDYYLRPMCCPHHHKIYASRLRSYRELPVRLCEYGDVFRYEKHGSLSGLLRV
                     RAMCMNDAHIYCMPEQVEDEFRAVIDMYLMYYGHLRMGNFRVRLSLHDKSSDKFVDNY
                     DEWERTEAIVRNVLNNLGIAYEEESGEAAFYGPKVDIQVKNLMGREETVSTCQLDFVM
                     PDRFDLSYIDAQGERKRPLILHRAPLSTHERMISFLIELYGGAFPTWLAPVQVRLVPV
                     NDEVMDYARQLESTLRNEFIRADIDTSHESFNKKIRNAVTRKIPNIWVLGKQELEGRS
                     VTWRRYAVKEQQTVPFDKAFDALKIMRDKRIMDNFADVNLPI"
     gene            13646..13771
                     /locus_tag="PKN92_08110"
     CDS             13646..13771
                     /locus_tag="PKN92_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48795.1"
                     /translation="MLHIPGRWRGYVFLSVSDLKSRIPETPNGQKIRLLKKFVPF"
     gene            13874..14677
                     /locus_tag="PKN92_08115"
     CDS             13874..14677
                     /locus_tag="PKN92_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ48796.1"
                     /translation="MAIREIIKIDEEKCDGCGLCIVDCEEGALAIVDGKAKLVKDSYC
                     DGLGACIGSCPTGALTIEVREADEFSLPPDHPAAQAAAAAAPAPAPASHAPSGCPSGG
                     AHPAAAAFSGGCPGSRSRQFQETSAPASPTTAGQTAAHTPSALTHWPVQLHLVNFNAP
                     QFQQREILIAADCSAFACGAFHPRLLQGKALAIACPKLDDPTGYLEKLTAMFQNAAPP
                     KVTVVRMEVPCCRGITQWVVSARAQAGSSVPVEEIIMGVEGDLLSRNTY"
     gene            14752..>16356
                     /gene="hcp"
                     /locus_tag="PKN92_08120"
                     /gene_synonym="priS"
     CDS             14752..>16356
                     /gene="hcp"
                     /locus_tag="PKN92_08120"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNZ48797.1"
                     /translation="MFCYQCEQTSRSTGCTAHGVCGKDPETAALQDLLLHMAKGISQY
                     AHKLRAAGVTTPKADHFVIKALFTTVTNVNFDAATIAELIRAGVAIRSELKESCKSMG
                     CTNELSGPATFAFAESQDEMVRQGETIGIESRLPKYGSDVTGLQELILYGLKGMAAYV
                     DHAAVLGVEDDSVYAFTHDTLSFLADEVDDVNALVARALAVGENNLKAMQLLDSGNTG
                     TYGHPEPTRVRVTPVKGKCILVSGHDLKDLAALLQQTEGKGINVYTHGEMLPCLAYPG
                     LKKYSHLVGNYGGAWQDQAKEFDAFPGAILMTTNCIQRPRQSYQSRIFTTGLVQWPGV
                     VHVTNDDFSAVINAALEAEGFTEDGPEQYITIGFGHNAVMSVADKVIDAVKAGKIRRF
                     FLIGGCDGAKPGRDYYTNLATSVPDDCVVMTLACGKYRFNKLEFGDIGGIPRLLDIGQ
                     CNDAYSAIQIAVALANAFECGVNDLPLSIVLSWYEQKAVAVLLTLLHLGIQGMRIGPS
                     LPAFVGPNVLDVLVKTFGLTPIDTPEA"
BASE COUNT         4182 a         4412 c         4190 g         3572 t
ORIGIN      
        1 cgtcataaaa gcagccagcg taccgccggc aagggcacgc aaacccagcc gagccacctc
       61 cgcccgcctt gcgggcacaa tagccgaaat gccgccaatc agaattccca ggctgccggg
      121 attggcaaag ccgcacagag cataggtggc aataacaaca ctgcggggac tgagactccc
      181 atcgatgatg tgagtctgca gctgctggta ggcaataaat tcattgaaga tggttttggt
      241 ggcaaggagc tgcgaaaaag cgggcaaatc attaaacggg acgcccatgg caaaagcgaa
      301 gggatagaaa aaccagccca gccactgggt cgccggggaa gaagtcagcg ccagggaagc
      361 ctgatccagc aggtgaatca acccgacaaa cacaatgagc atcgccgcca cgtggagtgc
      421 catattcagc cccagagaag ccccgcccac agcggcatca aaaatatttt cgctttccac
      481 cgggatagat acggagacat cacccagcgt ttcaggcttt tctgtttcgg gaatcatgaa
      541 ctttgaaatc aggatggcag caggtgcact cataagagac gccgccagca gatgcccggg
      601 ctccgctcca aaacttgcat aagccaccat gacgcttgcc gctatggtag aaaggaatgc
      661 caccatcacg gtaaagagct cggagcgggt catccgctgc aggtatgcgc ccactgcgga
      721 catggactca attcccgtaa aaacaagcaa ggctgtggca aaggtttccg cgcctgaagt
      781 cttcagtgta cgacgcataa gccaggcgca ggcacgaacg acggcctgaa taatgcccag
      841 atgctgcagg atggcggcaa taccggcgac aaaaataatg accggcagaa cattaaatgc
      901 aaaaatggca ttgatggcaa aaggttcctg cgcagccatc acgccatcag ggccgggaac
      961 cagaacccgc tcaagcataa aaaagcgtga gagattgcca aaaacaaatt ccgccccttc
     1021 cgtgctcgct tctgtcagca gatcaaaaac acggcgcaca ccgttaaaaa gccctgtgcc
     1081 cagtccggtc cgcagcacca gcagtcccag aagtgcctgc agtcctattc cccaaaaaag
     1141 aagccgccac tgaatctttc tgcgattgtc cgataacgcc caggcagcgc cgagcataac
     1201 ccataagcca cctgtactga gcagccgcaa cagcgtgtcc tgcataatac actccttccg
     1261 gcatggcagc tttatccggc acgatcaccg gtttttgcac tcgcccgggg aacaaccagc
     1321 acggggcatg cggcttttcg aaccaccttt tcagtcacat ttccaaaaag cacgttttcc
     1381 aatgcgctgt gcccgtggcg ccccagcaca ataagatctg ctctgatttc tccggcgtac
     1441 tcaagaatct gcagatagtc ttttccgata cgcatgacaa cctcaagcga tacagagtcg
     1501 ggcacatgcg ggcggtagtc tctttcaatt ttctggtcta tggcggagcg tgccttttcg
     1561 tccactcctt ccacattata gacgtaggtc ttccagaact gcgcttccgg ttccggaatc
     1621 acatgaagca ggtgcagaac ggaaccaggg cgccgcactg ccgcatccag tgcaaaagta
     1681 aaggcacggg ctgcattttc agaaaaatca gtgcagcaca ggatcgttgt gtaaggactc
     1741 accggcagtt catcacttgc catgacagca tcctccatcg ttggattatt ttaccagtcc
     1801 gggaagaaag agtgatatct gagggaacag cgtcagcagc acagcggcaa caatcagcgt
     1861 acagagaaaa ggcatagatc ctttaaaaat ggttgaaagg gggatgtcgc gctcgattcc
     1921 gcccaccaca tacacattta cgcccacggg cggggaaatc acacccattt gggtgaccac
     1981 aacaataacc actccaaacc agatgggatc atagcccagt tcaacgatca ccgggtaaaa
     2041 gatcgggatg gtaagcagca ccaatgccag tgcatcaaca aagcatccgg cgagcaggta
     2101 aaaaataatc accagaaaaa caatcattgc acccgggagc ggaagattgc tcagccagtt
     2161 tgccaactca taggggattc gggtaatcgc cagaaaacgc ccgaaaatca cggcgcctgc
     2221 tacaatcatc atcaccatgg aagaagtgcg ggctgtttcc agcagtgagc ggatcagaat
     2281 tttaatgctg agttttcttt tgaccagcgc aatgatcagg ctgcccgcag caccgaccgc
     2341 cgccgcttcc gtaggcgtaa aaaatccgag aaacattcct cccatcacca caataaaaag
     2401 aataagcgtc tcggcaacgc cggcaagaga gcgtatcttt tctcccagag aggcacgggg
     2461 acccaccggt cccatctccg gacgacgccg gcagttgatg tatatggtca gacagaaaag
     2521 tgcggcaatc atcagacccg gcagaacccc ggcaataaaa agttttccaa tagaggattc
     2581 tgtcagaatg gcatagacga taaaaaccac actgggcgga atcagcatcc ccagactgcc
     2641 acccgcagca accgcaccgc tggcaagttc catgctgtaa ttataccgcc gcatctccgg
     2701 gattgccaca gatgccatcg tcgctgctgt tgcggggccg gaaccgcaaa ttgcgccgaa
     2761 agccgtacag gctcccactg ttgccatcgc cagaccgccc ggaagggctc ccagccattt
     2821 gtaagcggca ttgaacagac ttttgctgat ccccgcatga aaagccacct gacccataaa
     2881 aacaaagagc ggaatgacgg tcaggctgta ggaggagaag gtttcgtaga gctccgctga
     2941 agccatactc atggcagccg tagaggtcgt cagcatggcg aagccgacaa taccgaccaa
     3001 tgccatcgca aaggcgacgg gcatgctgga aatcaggaaa aagacaaggg ctatgccgcc
     3061 aaggataccg gtctgaatgg gagtcatggc ttgatcatct cccggccggg gtgcagcaga
     3121 ttatgcagcg ttacaagcat ggtgagcccc agcgaaaaag caatgatgta tgaaagccag
     3181 aaaagaggaa cgcccagcgt gagtgacact tccccggagc gcttcagcgc gttgccgtac
     3241 agcagacact gccacgcaaa agtgccgaaa agagccataa tcacaagcct gctcaggctg
     3301 tcaatgacaa tcctgccgcg acgatgcagc ttcagaaaaa aatactctat ggcaacatga
     3361 ccttttaccg ctgtggtata cggcaaagca cctgctatgg tcagtgcacc gaaaatgcgc
     3421 accagatcat agcagccttt cagaggatac ccgcccaggc ggaagaggat gtccacgcag
     3481 gtaatgcaca tcatggcaag aatacataga ccggacagca ctgccagcgc atatacaaga
     3541 aagcgcatgc tctgccagaa aaagcgcccg gcagcctgca gagatttcag acaggaatgg
     3601 tggaatatat tcatgtacta tttagcggga agcagccgct atccccttct gcaggtcggc
     3661 aagaaattcg gctccgggca ggtttttggc ggtacaggct gcagtgtact catccagtat
     3721 aggctgcacc gcttcgcgcc agcgcgcctg ctcttctgcg ctcaatgccg tttccgtgcg
     3781 cccgagttcc cgtatgaaag ccagcccttc ttcatctgct ttgtcccagg cggcaccatg
     3841 gcggtcaacc cattcttcac tgactgcggt gatgatattc tgaatgtccg ccgggaggct
     3901 gtcccatttc cctttattca tggtgacgaa catggctgtc gtgtaaccaa tcacagagct
     3961 gtctgtaatg gacgaaatca cttcgccctg cttccagcct ttcagtgttt ccacagggca
     4021 caatgtcgcc tcaacaacgc ctttctgcaa ggcttcatag gtttcaggct ggctcatgcc
     4081 gaccggcgaa ccacccaacc gctcgataat tttggcggaa aggccggtgc cgcgcacttt
     4141 aagaccagcc atatcttcca gagatgcaac aacttttttg gaggcgagaa tgccgggacc
     4201 gtgagcatgc acatacagca gatgggtatc ggatgtctcc gcaggcgcat atttctttgc
     4261 cagatcagtc gcaagccgtg tggcagtttt gccgtcggga taacccacag gaagatccag
     4321 cccttccaga agaggaaagc gtcctctggt ataggagaag cagctcatgc ccagatcaga
     4381 aatgccggaa acaacacctt cataacattg cggcgcctga ctcaaagatc cgcccggata
     4441 aatgtttatt ttgactctgc catccgtgcg ggcttccact tcctttgccc aggcttccgc
     4501 ttcaaggcat tgcgcatggg taggaggaaa gaaaatgctg taggacagga caatgggttt
     4561 tgcttttttg gcagaaccgt ccgaaggtgc cgggctgccg catcccgcca ttaccagcag
     4621 cgaaccaacc gaaaatagag atacaacaca agtcaagaca gcgatttttc tgaacatggc
     4681 aattactccc gttttaccgt cgcaaacatg ttctgcggca gaagtttcga tgtctgcagt
     4741 atagcccagt aagccgggcg tgcgcaaaat aactgtttca cttttacttt gcattgatcc
     4801 atattgaaac ggtttttccc gtgtatgcgc ccttatcttc aaaaaacaca cgccaaagcc
     4861 tgaaatctac cccgtttcct ttttggcatg gtttgtgctc tgtaaagagg tacaagacga
     4921 caccattaac cgaaaggaga aagaacatgt caaaaacaat cggaattgac ttaggcacaa
     4981 ccaactcgtg cgttgccgtt attgaaaatg gcgaacccgc cgtaatcccg aatctcgaag
     5041 gatcccgtac cacgccttcc gttgtggcat tcacacggga cggcgaacgt ctggtgggca
     5101 cagcggcctg gaggcaggct gtcaccaacc ccgccaacac cgtcttttcc atcaagcgtt
     5161 ttatgggaag acgttatgac gaagcagtca gtaagcatga aaatacgccc tacagtgtga
     5221 gccgcgcttc aagcggtgac tgtcagatag acattcaggg caagggatac cgtccgccgg
     5281 aaatctccgc catgatccta cagaaactga aggaagccgc agaaagttat ctgggcgaaa
     5341 aggtcacccg agccgtcatt acggtaccgg cgtattttaa tgatgcacag cgtcaggcga
     5401 caaaagacgc cggccgtatc gccgggctgg aagtggctcg tataattaat gagcctactg
     5461 ccgccgcact tgcatacgga ctggacaaaa agaaaaaaga aaaagtggct gtttatgatc
     5521 tgggcggagg cacctttgat atatctatcc ttgccattga agaggacagt tttgaagtgc
     5581 tgagcaccag cggagacacc catttgggcg gtgacgattt cgaccaggcg gtcatcaacc
     5641 atctggcaga tgaattcaaa aaagagcagg gcattgatct ccgcaaagat ctcatggcgc
     5701 tgcagcgttt gaaagaagcg gctgaaaaag cgaaacgtga attgtcgtcc accctgcaga
     5761 ccgatgtgaa tctgcccttc attaccgccg atgcctccgg gccgaaacat ctgaactgcc
     5821 agctgacccg ggcaaaactg gaacagctga cagcggcact tattgatcgc acaaaagttc
     5881 cctgcgccaa ggctcttgca gatgccggtc ttcaggccgc tgacattgat gaagtgattc
     5941 tggtgggcgg catgacgcgt atgcccgctg tggtgcagac tgtaaccgac ttcttcggca
     6001 aagaaccaca tcgcggagtg aatcctgatg aagtggtcgc cattggcgcg gcaattcagg
     6061 gcggtgtgct ttcaggagag gtgaaaaacg ttcttcttct tgatgtgacg ccgttgtctc
     6121 tgggcattga gacgctgggc ggtgtcttca cgcgcctcat tgaccgcaat acgaccatcc
     6181 ccgtatccaa aagcgaaatc ttcagcacag ctgaagacaa tcagaacgct gtgaccgttc
     6241 atgtgcttca aggtgaaagg gatatggcgg caggcaacag aaccctgggc cgtttcaacc
     6301 tggagggcat tgctcccgca ccccgggggc tgccgcaaat tcaggtgtct ttcgacattg
     6361 actcggacgg catcctgcat gtctctgcga aagatcaggc gacgggccgc gagcagaaaa
     6421 ttcgcattga ggcttcgtcc ggactcagtg aagccgaggt gaatcgcatg ataaaggatg
     6481 cagagcagca tgccgctgat gacaaaaaac tgcgcaaaga agcagaaaca cgcaaccagg
     6541 cggaacatct ggctttctcc gcggaaacac tgctgcgtga gcatggtgat aagattgctc
     6601 cggatcagaa aaacgctctg aaaacggcac ttgccagcct gaaagatgcg gttaaacaca
     6661 atgatctgga agcaatggaa gccggcttgg aaaaagtgtc ccagatttct caggcactgt
     6721 cgcaagccat gtacgctgat gccgcaggaa aaacaggagc atcaggttcc cactgcggat
     6781 ccggcgccag ctgcagcacg cctcctcctg cctcttccgg cggaaacacc atggacgccg
     6841 actttaccgt cctcgacaac taaaccaatc tccctccttc ctgccgccgg acgcgctcct
     6901 caggagatgc cgcccggcgg cctcatttct cttccctctg aaaaatcatc tttgcttcat
     6961 tctgctgatt tcatcaagta agggcggata ataagcggac acgatatgtt cccaatcata
     7021 cacgtcctct gcccgctgcc gcccccgggc tcccattgcc gcacacagat gccgatcttc
     7081 aaggagtctc tcaagacatg cggcgagcgc ccgactgttg ccccgttcaa aaaggaagcc
     7141 cgtctctccg tgaacaacaa tatcctgaag gccccctgtc cgggatgcgc aaacagcaat
     7201 cccggacgcc atcgcttcca gcgcaaccag cccgaaaggc tcttcccata aactcggcac
     7261 gacacaaata tccgtattcc ggtaggcttg aggcatggag tcataatcca gcttccccac
     7321 cgccttcagc cagggcggac cctcatagcc ttcaggaaga gttgcccgaa cttcaaatgc
     7381 aattcctgaa cccgacacct tttccgctgc ctcaagaaac agagaaaagc ccttggcggg
     7441 atcctctatc ctgcccggca caaatagcac aggcaccgga cgcccggcac gttccggcga
     7501 aggcagaaac cgcctgatat cgacaccgtt gggaatcacg gcgatctctt tggtcagcag
     7561 ttccaccggt gcgcgcatgt gttcggtggt cacaatcact ttgctgcact gcgccattgc
     7621 ctttttaaag cggctgtaat agtcttttgt ccacgcccga ctttccacat actccctggt
     7681 ccaggcatgg gccgttcccg caaacaggct ttcacgctgg ctggctagcg cacagcggcg
     7741 acatatgtcc gggctttcca cataacaatg aggacagggc gttccctcgc ggaaacggag
     7801 gatgtctttg tggcagctga gctcatgggc ataacatcgg gaaatgatcg ggtagtcttt
     7861 cagagcttca ataagggctg tcttaagaaa ataaccctgt gtcagaaaga caatatccgg
     7921 ggaaaaaccg acaacagctt cgtacaaccg gttgtgcagg aaagcctccg taagcccctg
     7981 ttcacccaat tctaaaaggg tcaccgggaa cggaagggca ccatcgcgaa tatcgccccg
     8041 ctcccaactc ccctcctctg ctgttacaaa aagatgcgta tcataaccga gacgcatcag
     8101 ttcctgcatc acataataga cgtccacatc cgctccgcca tgaggcggcc aggaaaacag
     8161 caggtctaca acggcaatgc gcatgaaaag acactcttcc ggattcatag atacttcaac
     8221 aggacacagt aagcagcgcc tattattaca aatctgttcc tttaatggaa acgttatttc
     8281 cttgtctcag gcaaatcaat tgtccccatc cggcctcaat agtaagccaa ggtacaatta
     8341 aacagggaaa gtttctttcg ttgctgcgaa tatgtataat gatttacgaa ttttccgctt
     8401 tgcttctcaa ccctttaatt ggaggctgga aagtggcaga aatacctgaa ctcgagtaca
     8461 gtgtcatcgc cgaccgttat gttgtccggg aaaagctcgg gcgcggagga atgggatctg
     8521 tttatcttgt ccaggataaa caaaccgggc agatggtcgc tttaaaaacg ctgcacacca
     8581 aatatgccaa cagcaaacat gccatcgcac ggttcatccg ggaagtcaat acttcgcgaa
     8641 agttgaacca tccgggcatt gtaaaggtat atgaagcgag caagtggaac aatacgcttt
     8701 tttataccat ggagtacatc cgcggcaaat ccctccgtcg ctggctgcag gagcggcaca
     8761 atcttgaatt taattcagtg gtgcgtgtcc tttgcctggt cgccgatgcc ctgcagcatg
     8821 cccatcagat taccattcat agggatctct ctccggaaaa catcatggtt cttcctgatg
     8881 gatccatccg cctgctggac ttcggtctgg caaagctcga tgaccagttc aaagggttga
     8941 cagtagtcgg tgccaatctg ggcaaaattc attatatggc acctgaacag cagctgaacc
     9001 ctgccggagt tgactacagg gcggatattt tttctttggg cgtgatgttt tatgagctgc
     9061 tgtgcgggcg ttccccccag tccggctata aagtgacgga gatccgtccg gaactgcctg
     9121 tggaatgcaa cgcatttgtg gagaaggcga ctcaccctga tcccaacctt cgttttcagt
     9181 catcgcggga gttcagagat gcccttctga atctctacag aattcaggaa gcacgcacca
     9241 agaagaccgt taaaaagcct gaagggctgt ttggcaagat cagcgcgttt tttacaaatc
     9301 ttttttctcg ccctcaaaag agcggctcac aacgcaagcg gggcagataa gatgtattcc
     9361 atgggaaaaa ccaaagccat tgcttcagcc actattcttt tatctcttgt gctctttcag
     9421 acggacggct gcgccgaaaa tgatgaaatt acagatcatt accggcgtat gatggtgtat
     9481 cacgaataca tggacggcaa tgtgcccgac ggcgctgttg tttttattgg tgacagcatt
     9541 acacaggggc tctgcgtctg tgcagtaacg ccccttgccg ttaattacgg catcggaagc
     9601 gataccactt acggggttct gcaaagattg cccaagtata aatcgctgga acactgcgcc
     9661 gctattgtct ttgccattgg cgtcaacgat ctttccagac gcagcgatga cgaaatcatc
     9721 agcaactgga aacttattat gcaaagcatc ccggaagata ttcccgccat tttcagtgcc
     9781 atgcatcctc tggatgccga aacgaaaaat atctccgaag aatccaatca aaggattgtc
     9841 cggctaaatg aggcgctgaa aaacttgtgt gcagaaaaca atcagtattt tgccagtgca
     9901 tttacgaaac ttgtcaatga aaagggcaac cttgatccgg cttttcatac gggcgatggc
     9961 gtgcacctca acacagcggg atatcaagtg tggattgctg tgcttcagac tgcactgcaa
    10021 agcattccgc aggaataaca cagtatccct cccccccccg tccgcgcctc acaaattcat
    10081 ttcaatttat cttccagaaa acacagatat gggttataat aagtgcactg tcttgtgcat
    10141 atccgcaccg acaaaaccgt ggagggtagt ttcatgtcag atgatctcac cgccagattc
    10201 gaacgggcaa gcaaagaagt cacagaattg tctgaagctc cggacagcgc ggcgcagctg
    10261 gaattgtaca gcctttacaa acagggaagc gtcggcgact gcacaggtga acgcccgggt
    10321 atgcttgagt ttattaagcg tgccaaatat gatgcctgga aagagcgggc gggcatgagt
    10381 caggacgagg caaaagaaaa atatatcgcc ctcgttgagc agcttaaaga agcagacaaa
    10441 aaataaacac ggcttccgga tccgagcgca acagcccggc ggcggacatt tttatttcat
    10501 cgcactttct ttccatggta tgattatcgc atacggttac cggtgtatag ccggatggaa
    10561 aggaaaaacc atgaatgtcg atatccgtcg ctttctctgt gggctgcctg ttttcctgct
    10621 tctctgcatg tttcccgtat ccgctttcag cgccgatgct ggagccgatg ccgatccgga
    10681 caacctttct ctttttaact cgctctcggc agcacaggtc ttcgaggcgg cccgggcatc
    10741 tgtaaaagaa actttttcag ggataccgga aagttttgaa tccggtgatg tgcttgtggt
    10801 tctccgcaag caggaaaaag tgacggtccg cctgtacggt gtagactgcc cggaagaggg
    10861 acagcctttt tttgaagagg ctaaaaccgg cggacattct tttctaggcg gggaagccgc
    10921 agaaatctcc atcctgtccg ttgacaacaa agatcttcct gtcgtcctga tcctgaataa
    10981 agaaaaacaa agtctcagcc attaccttgc ggcgaaagga ctgggctggt gggatgaaaa
    11041 gaatgcaccg aaagacgctt tgctccggaa attgagtgcc ggcgccgttc tgaaaggaca
    11101 gggtattttt tcgcaggcaa caccgctggc gccctgggat tacaggaaaa gcaaagaaat
    11161 agaggacttc acctatacgc tggaaaccgt ggcagaaccc gttcccgaca aaaaaaccgc
    11221 tcgttccgat tctgagtcat cggaaaagtc cctaagcaaa aaaggcacaa tggttgaaag
    11281 cgcaccgaga accgcgccag tacaactgaa ggcccctgct gaaaagatcg atgccggtgc
    11341 cttgatgatg cgccatcagc cccgcatttc tcgggatgaa tccggacggc cccagggctt
    11401 gacagctaca gacatcagtg ccatacccta tgcggcgcag ctcggctttc agaacggtga
    11461 tatcatctcc cgtgtaaatg gcatcgccat agagagcgag gcgcaaatta tggggcttat
    11521 ccctcaattt caaaatgtca aacaatttca ggtggaagta atccggaacg gccgcagcat
    11581 aacaattcca atttccatcc cctgaataag catttggatt cactatttgt caccgtgata
    11641 tagtgtttgt ggtgcgtatg tgtttttccc gtccggcaac aagccgggcg acgtgtatcc
    11701 cttcagcgtc tgctgcccat aacagcgcaa tcaggggatc agatcttttt acaggatgtg
    11761 tgttttatga ataactgttc agtttcagtt gcggacaaaa aagaaaccgc attgtatcgg
    11821 ctgcgccatt ctctttctca tttgcttgcg gcagcagtgc tggaaatccg gcctgatgcc
    11881 aaactggctt tcggcccgcc tgtggaaaac ggcttttatt acgacttcga tttcggcggc
    11941 acgcctgtag gagaggcgga tctggcggat atcgaaacac gcatgaacgc cctgattaaa
    12001 aagcgcattc cttttgtccg gacagagatg ccttttgaag ccgcagtcaa gcatctggaa
    12061 gcgggcggtg aagtctacaa aatcgaatat gcccgggaac ttttagataa aggactggct
    12121 gttaacggct ctctttcttt ttatacaatg ggcgacttca tcgacatgtg cgaagggcct
    12181 cacctggaac atactggcga agttccaaaa aagtctttta aactggaccg catttccgga
    12241 agctactggc gtggcgatga aagcagaccc atgctgaccc gtatctacgg tctggcattt
    12301 gaatctgcag acgccttgaa agagtatctg gaaatgcgca aacttgccat ggagcgggat
    12361 caccggaaac tcggtgatga actcgatctt tttatccagg attctgaagt cggtgtaggg
    12421 cttccccttt ggatgccgaa cggcaccatc atccgcgatg aactggaaaa atgggcgcgt
    12481 gaaacggaat tcctgggcgg atatcagcgg gtttcaacgc ccagcgtcac ccggaaagaa
    12541 ctctatctgc gctcaggaca tgtaccctat tatcaggaca gcatgtatcc gtccatgccc
    12601 gtcgacgagc acgatgacta ttatctgcgg cccatgtgct gcccgcatca ccacaaaatc
    12661 tatgcttcga gactccgcag ttatcgtgaa ctgccggtgc ggctctgtga atacggagac
    12721 gtgttccgct acgaaaagca cggctctctt tcagggctgc tccgagttcg cgccatgtgc
    12781 atgaatgatg ctcatattta ttgcatgccc gagcaggtgg aagatgaatt ccgggctgtg
    12841 attgatatgt atctcatgta ctacgggcac ctgcgcatgg gcaacttccg ggtgcgttta
    12901 tcactccacg ataaatccag cgacaagttt gttgataatt atgatgaatg ggagcgcacc
    12961 gaagccattg tccggaatgt gctgaacaat ctcggaatcg cctatgagga agaaagcggc
    13021 gaggcagcct tctacgggcc gaaggtggat attcaggtta aaaatcttat ggggcgtgag
    13081 gaaaccgtct ccacgtgcca gttggacttt gttatgccgg atcgcttcga tttgagctac
    13141 attgatgcgc agggagaacg aaaacggccc ttaattctgc atcgcgcgcc cctcagcaca
    13201 cacgaacgta tgatctcttt cctgattgag ctgtacggcg gtgctttccc gacctggctg
    13261 gcacctgtcc aggtgcggct ggttcctgtc aatgatgaag tgatggacta tgcgcgacag
    13321 ctggaatcca cgcttcgcaa tgagtttatt cgggctgata tcgacacaag ccatgaaagc
    13381 ttcaacaaaa aaatccgcaa cgccgtcaca agaaaaatac cgaatatctg ggttctcggc
    13441 aaacaggaac tggaagggcg cagcgtcacc tggcggcggt atgctgtgaa agagcagcag
    13501 actgtacctt tcgacaaggc ttttgacgca ttaaaaatca tgcgcgacaa gcggattatg
    13561 gacaattttg ccgatgtaaa tctgccgatt taaacggatt tacctgaaag ctccgcattt
    13621 tctgctgaca gcaatcctct gcgccatgct gcacatacct gggcgctggc gtggctatgt
    13681 ttttctctct gtatccgatc tgaaaagtcg tatccccgaa acgcctaacg ggcagaaaat
    13741 cagacttctg aagaaattcg tgcctttctg aaataaatga tgcctatttg acttaagtca
    13801 aagaactgct gcgcccgtcg ggttatacta cggcaacaaa gcagaacgct gcataaagaa
    13861 aagggtcaga tttatggcta tcagagagat tatcaaaatt gatgaagaaa aatgcgacgg
    13921 ctgcggtctt tgcattgtag actgtgaaga aggcgcgctg gctatcgtag acggcaaagc
    13981 caaacttgtc aaagacagtt actgcgacgg cctcggtgca tgcatcggca gctgccctac
    14041 cggggcattg accatcgaag tgcgtgaagc agatgaattt tctctgccgc ccgatcatcc
    14101 tgccgctcag gcggctgctg ccgccgcgcc cgcacctgct ccggcaagcc acgccccgtc
    14161 gggctgcccg agcgggggag cacatccggc tgctgccgct ttttccggag gctgccccgg
    14221 ctcccgttca cgccagtttc aggaaacttc cgcgcccgct tccccgacaa cggccgggca
    14281 aacagcggcg cacactccgt ccgcattgac ccactggccg gtgcagctgc acctggtcaa
    14341 ttttaacgct ccgcagtttc agcagcggga aattctgatc gctgccgact gcagtgcttt
    14401 tgcctgcggc gcttttcatc ccagactgct gcagggcaag gcgctggcga ttgcatgtcc
    14461 gaagcttgat gaccctaccg gctatctgga aaaactgact gctatgtttc agaatgcggc
    14521 accgccgaaa gtgaccgttg tccgcatgga agtgccctgc tgccggggca tcacgcagtg
    14581 ggttgtttcc gcacgcgctc aggcaggcag ctcggtgcct gtggaagaaa tcattatggg
    14641 tgttgaaggc gatcttctga gccggaatac ctattgacgc tatctttcgt gtatcctatt
    14701 tgttcgtgta tacagtgtct ggttcctacg aaagaggaaa atactatgtc gatgttttgt
    14761 tatcagtgtg agcaaacgag ccgctctaca gggtgtacag cccatggcgt atgcgggaaa
    14821 gatccggaaa cagcagccct gcaggatctg cttttgcata tggcaaaagg catcagtcag
    14881 tacgcacaca aattgcgtgc cgccggtgtg accacgccta aagcagacca ttttgttatt
    14941 aaggcgctgt ttacaaccgt cacaaatgtg aactttgatg ccgccaccat tgctgaactg
    15001 attcgtgccg gtgttgcaat ccgcagcgag ctgaaagagt cctgcaagtc aatgggctgc
    15061 accaacgaac tgtccggccc tgccactttc gcattcgcgg agagtcagga tgaaatggtg
    15121 cgtcagggag agactatcgg catcgagtcc cgcctgccca aatacgggtc tgatgttacc
    15181 ggtttgcagg aacttatcct ttacggactg aaaggcatgg cggcgtatgt ggatcatgcg
    15241 gcagtgcttg gcgttgaaga tgacagtgtc tatgcgttca cccatgacac gctctctttt
    15301 cttgcggatg aagtcgatga tgtgaatgct ctggttgccc gtgctcttgc agtgggagaa
    15361 aacaatctga aggcaatgca gctcctcgat tccggaaaca ccggtactta cggtcatccg
    15421 gagccgacac gcgtccgtgt gactcctgta aaaggaaaat gcattctggt atccggtcat
    15481 gacctgaaag atcttgccgc actgctgcag cagacagaag gcaaggggat caatgtgtac
    15541 acgcacggcg aaatgcttcc ctgccttgcc taccccggtc tgaaaaaata cagccatctg
    15601 gtgggtaatt acggcggtgc ctggcaggat caggcaaaag aattcgatgc cttccccggt
    15661 gccatcctga tgacaaccaa ctgcatccag cgcccccggc agagctacca gtcccgcatt
    15721 ttcaccaccg gactggtaca gtggcctggc gttgttcatg taaccaacga cgacttcagc
    15781 gctgtaatca atgcggcgct ggaagcggaa ggctttacag aagacggacc cgaacagtac
    15841 atcacgattg gcttcgggca caatgctgtc atgagcgttg ccgataaagt catcgatgcg
    15901 gtcaaagccg gaaaaattcg ccgtttcttc ctgatcggcg gctgcgacgg cgccaagccc
    15961 ggacgtgatt attacaccaa ccttgccacc agcgtccccg atgactgtgt tgtcatgacg
    16021 cttgcctgcg gcaaataccg cttcaacaaa cttgagtttg gcgacattgg cggtattcct
    16081 agactcctcg acatcggtca gtgcaatgat gcctacagtg cgattcagat cgccgtggcc
    16141 cttgcaaatg cttttgaatg cggtgtcaat gatctaccgc tgtccattgt gctcagctgg
    16201 tacgagcaga aagctgtcgc cgtgcttctg acactgcttc atctgggtat tcagggaatg
    16261 cgtatcggac cgtccctgcc tgctttcgta ggccccaacg tgcttgacgt tctggtgaag
    16321 actttcgggc tgactcctat tgatactcct gaggcc
//
LOCUS       DAOPWB010000074        16247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3649_length_16247_cov_5.692194, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000074 DAOPWB010000000
VERSION     DAOPWB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 16247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16247
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3649_length_16247_cov_5.692194"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PKN92_08125"
     CDS             <1..532
                     /locus_tag="PKN92_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNZ48798.1"
                     /translation="LLRMGPFPYARWEEIHPNTVQSPLYLVLDRIQDAHNFGAIIRSA
                     ASLGASAVIVGESRQAPVNSQVVRASAGAVMHLPLVQVSDCLQQARQMKEQGISLIAT
                     ALNAEKAVWECSFKRPVGLILGNEAEGVDRALLELCDESCKIPQSSGIDSLNVAAAAA
                     AILYESWRQKNIDTRV"
     gene            594..1241
                     /locus_tag="PKN92_08130"
     CDS             594..1241
                     /locus_tag="PKN92_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNZ48799.1"
                     /translation="MTLSILISIIGLIIAAVICLLLSLSRDHYLVGAFKMVASTGYLV
                     LCIAAGGLHSAYGLAVFIGLIFSWWGDLFLISKTRSIFLLGLLSFLATHATYTTAFIM
                     QHPGKSAPVAALSIMLVPGIFVGRWLYPYLKEMRVPVLLYMTAITVMVAFAAGVFLTR
                     GYVITLAGAGLFYVSDLFVARYHFIAPGKINQVIGLPLYYAGQILLSLSVIYAYT"
     gene            1338..1919
                     /locus_tag="PKN92_08135"
     CDS             1338..1919
                     /locus_tag="PKN92_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNZ48800.1"
                     /translation="MVQTASTMMPLGTPAPDFSLPDTQKQTVSLKNYADAPGLLVMFL
                     SNHCPYVKLVREEIARIGREYGAKGVGIVAVMANDVQAYPDDAPDKMALEVKDAGYTF
                     PYLYDETQEVAKAYGAACTPDFFLFDAQKRLVYRGQMDDARPGNNRPVTGADLRAALD
                     ALLAGKPVSDVQRPSLGCNIKWKPGNAPAYFSS"
     gene            complement(1904..2890)
                     /gene="thiL"
                     /locus_tag="PKN92_08140"
     CDS             complement(1904..2890)
                     /gene="thiL"
                     /locus_tag="PKN92_08140"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNZ48801.1"
                     /translation="MSDLQNLGEFGLIYFLTEGLPPDKTVIKGIGDDCAVVRIGGEEL
                     LFSCDAFLEGVHFQRHWATGEDIGHKAAATALSDIAAMGGIPLYALITVAAPPDTDIS
                     FLQDVYTGLKHVFSDSQCSLIGGDTVSSASGLFLNVTVTGKGAGGRILYRRGMMPGDL
                     IMVTGHPGASAQGLHSLLHEKDAPPSLLQAHRRPVPRLQEGLFFSGDPAVHAMIDISD
                     GVSQDLMHMAQCSSVSVYLNSGDFPFSPDQIAYGNAGGLPALDAFLYGGEDYELALAV
                     DPEQADALQKRFSSMFPLPLTGIGHAAAGDPELWMDGKTIKSGGWDHFQELK"
     gene            complement(2887..3783)
                     /locus_tag="PKN92_08145"
     CDS             complement(2887..3783)
                     /locus_tag="PKN92_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNZ48802.1"
                     /translation="MVQLLNHKEVRHLLPLRPPDGHKGSFGHLAVAAGSTGFVGAVKL
                     ACLGAYRSGAGLVTACVPAAIFDSLASSLTEVMLKKCSSTAEGSLSEAAFSELMSFCG
                     DKSTLLLGPGLSRHEETVRCVRRLCREITLPLVIDADALNALAEHKDDMKDAFHLREE
                     PVIVTPHPGEMSRLAGLPTAYIQKNRIPVALEFAQRWQVITALKGKDTIIAAPDGRVA
                     LCPLGNNGMATGGSGDVLAGIAGALLAQKIPAFDAACAAVYVHALAGDFAARRLGTRA
                     LIAGDIIDALPPAWQALEGYGL"
     gene            4082..5272
                     /gene="dinB"
                     /locus_tag="PKN92_08150"
     CDS             4082..5272
                     /gene="dinB"
                     /locus_tag="PKN92_08150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNZ48803.1"
                     /translation="MRRILHIDMDAFFASVEVARNPQLRGKAVVVGGNSEDRRGVVAS
                     ASYEARAYGVRSAMPLGRARQLCPHAIFIRGSHGVYGEVSRRIRDILYSVTPLVQMAS
                     IDEANLDITGSIHLFDDEAALALSLKQRIYEKEQLTATVGIASNKMVAKIAANQRKPD
                     GFLAVPNGEEASFLAPLSVAELPGVGPRSREMLEERGILTVGQLAAVPEAVLSRLMGA
                     QAASGLYAAAQGVGSDIVDPVRTPKSISRETTFSEDLQDWKELEVVLFCLAEHCAHTL
                     RSEGLEAGKVTLKVRYSDFETKSFGITLPEPTCLDTRIVEALKLLIPKAQQRRTRVRL
                     IGVNLSGLSKDQHQMRLFGREDSEKWERLLHAVDGMREKLGFDSMRLGNTLKVKKKED
                     PSPE"
     gene            complement(5286..6971)
                     /locus_tag="PKN92_08155"
     CDS             complement(5286..6971)
                     /locus_tag="PKN92_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNZ48804.1"
                     /translation="MSCKKIVYTLSATAILLSFSSLAPGAELQLWSVDPLVKVFRDAE
                     PQPAGTAVDHVARGEYGEFQIVVRSDTSLDTLSASVTDFSNAVSGTTIPGASVRFVGY
                     VHVDRPSQRPSLDQLRQPPADFPDVLLEDASVSVPSEQAQPVWISVHVPPAVPEGAYQ
                     ASVTVRSDSGGITQEATYPLKLVVYPITVDSTRLWVTNWFSMQWRHMEINPEPDSAAY
                     YDLLRRYARNMAEHRQNAALISPLALSRFYVNAEGALAVDFEKFDRWVQIFIDEGVIG
                     RIEGGHIGGRSGGWDSDFITYIHSVQDGAVTNGSAAPDSEEARTFYGFFYPALTAHLK
                     EKGWLDIYMQHLADEPTQRNMGTYKALAALVHEFAPELRIIEANHTKELVGSMDIWVP
                     QLNFFHEDFGHYQERQKNGDEVWFYTCVFPQGEYANRFIELPLIKTRLLHWINFTSGA
                     TGYLHWGYNAWKNEDPRVYTTREHNGPPYLPAGDPWVVYPGKEGPLDSIRFEAMRDGI
                     FDHELLCRLAEKNPEEAHRLAAGHILDFDRYNTDVEAFRATRIALLQALAAAS"
     gene            7170..8339
                     /locus_tag="PKN92_08160"
     CDS             7170..8339
                     /locus_tag="PKN92_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNZ48805.1"
                     /translation="MYKNLFVVGLLCCCGAVLGCVSAKGAGNTVCAAEKTAVLTPEAR
                     LASLKPEEALSAAAFEEKVTGAGGSVSFAFGSERSFAQCHASTLVETADGKIITAWFG
                     GTAEKNPDVGIWYSVFDGSEWTAPVAIAKVEETAHWNPVLFKDEKDVIHLFFKVGVDV
                     PRWSTWWMSSADNGKTWSEPLVLVPGDVGGRGPVKNKAIILSDDRWCAPASTEEGKWD
                     SFVDFSSDGGKSWQRSMDFPLDRRKFKGKGTIQPTLWESAPGHIHALLRSTCGAILRS
                     DSTDNGVTWCDTYDTGMPNNNSGLDLIQLEDGRLLLVLNPVGVNWGARTPLSLAVSSD
                     NGDSWSILAHLEDAPQDTEFEFSYPAIVKTAKGVAISYTYKRDRVRCWQIPLEAL"
     gene            8409..8822
                     /locus_tag="PKN92_08165"
     CDS             8409..8822
                     /locus_tag="PKN92_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HNZ48806.1"
                     /translation="MLKQRLIHPDILEALAAAGHGSKILITDGNYPASTRLGDNAGLV
                     YLNLAPGMLSVTDVLKVILTAIPVEEAAVMEPETGPEPSIFNEFRALLPDVPLQAYNR
                     FEFYEEASGPDTCLQIVTGEQRIYANLLLTIGVVA"
     gene            complement(8890..9633)
                     /locus_tag="PKN92_08170"
     CDS             complement(8890..9633)
                     /locus_tag="PKN92_08170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNZ48807.1"
                     /translation="MTRKKRIAAALLLSAALLLTISIIGAPDRLPESEHIARNQVALR
                     LNQIYAEEKAAPEADADASSSTQKDSDMTLVKTPDVFKVKFECTNGDFVVECHRDWAP
                     LGADRFYELVQSAFFDNSSFFRVIPNFVVQFGLAADPAVTAKWKNNRIKDDPVTESNK
                     EGYITFATSGPNSRTTQLFINTGNNQRLDTMGFSPFGKVIEGLDIVKAINSEYSEKPN
                     QGMITKDGNTYLKTNFPNLDYIKKATLIP"
     gene            complement(9907..11217)
                     /locus_tag="PKN92_08175"
     CDS             complement(9907..11217)
                     /locus_tag="PKN92_08175"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNZ48808.1"
                     /translation="MTKPLSFSEQEILNTTSAIVLGGGRGTRLYPLTKVRSKPAVPLC
                     GRYRLVDIPLSNCINSGINRISVLTQFNSHSLNRHINRAYKFDEFNGGFVEILAAEQT
                     NETGDWFQGTADAVRKHLYHISQSRTSHFLILSGDQLYRMDYRTLLKTHIEKDADITV
                     AALPVSREAAQGFGIMKVNRNAGIRRFVEKPSDDALLDSLITPSKVFDDFGLSADGKD
                     YLASMGIYVFKAHVLADLLLSHPEWVDFGKELLPNSLKSHKVFAHMFYGFWEDIGTVR
                     SYFDVSMAMVSSRPPFQFHDPNNVIYTHARMLPGVRIDRSTTINAILCSGTRIEQALI
                     EDSIIGIRSIVRPGAVIKNSVVLGADYFEEAGSTLKNPIGIGRNTQISRAIIDHNARI
                     GDNVVIRGSKRLKDTDGDGFAIRDGIVVVLKDAVIPKGAQIGDV"
     gene            complement(11615..12265)
                     /locus_tag="PKN92_08180"
     CDS             complement(11615..12265)
                     /locus_tag="PKN92_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48809.1"
                     /translation="MDDVLGNILFTFFLAGILILGVSLYFYYQYLIRRAFIETAKKHG
                     YRYYYRSYAIPQRFAFLAQHRRGRGRYAFNILIGQDKNSSRLVFDYTFSTGLGVEKKW
                     HYSSFAALRHGKTCPFLRIYPRSMLSVLGDIVGYDEALFEESEFTQKYAIYTTDESFA
                     HAMITQPLIEYLFRHPEQSLEVEPYWIALGTSERLVPEEVPRRVRQIEKVRAILPL"
     gene            complement(12312..13502)
                     /locus_tag="PKN92_08185"
     CDS             complement(12312..13502)
                     /locus_tag="PKN92_08185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008986821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HNZ48810.1"
                     /translation="MFETLQMAPADSILGLTEAFKKDSNPNKINLGVGVYKDAAGNTP
                     VLESVKRAERILVEEAETKSYLSITGSPAFANATRTLLFGKSHSVTENGRAYTANTPG
                     GTGALRVAGDFLYRWNPKGRVWLSNPTWANHGAIFEAAGLETKAYTYYDPASKDIDFD
                     GMLASLGESAPGDVLLLHACCHNPSGVDLSQEQWLTMSELIREKSLLPLIDFAYQGFG
                     AGLDEDAVGVRLLSEQLPEVLICSSYSKNFGLYQDRVGALTLVAADADSGARAFSQVE
                     KAIRANYSNPPAHGSLVVTTILESDILRGMWEEEVAGMRKRINEMRALFVDTLQSLGV
                     KQDFSFIRRQHGMFSFSGLNKDQVELLRTRYAIYIVGSGRISVAGMTEKNMPALCNAI
                     ADVL"
     gene            13782..15722
                     /locus_tag="PKN92_08190"
     CDS             13782..15722
                     /locus_tag="PKN92_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48811.1"
                     /translation="MIKHCFMLAVVLIGSMIASADQFVFTSEEPIDLTAPPSAAVCHF
                     LQEDFSPFREAFVQNGKAVLLPGELPVGWYKVDFLNAEGKLLDWTSIAVLIPLLKRPS
                     PQTPVAIDVALSWVPPPGEASWRSVARMAALAGVSMVRDRILWRDLQPEPGPLVAYTK
                     YDDTSVIQKDAGLDILQVFHDVPYWMLGYPQGKRHIPEDLRLVWRFCREASARYKDTV
                     QAWQPWNEGNTRTFGGHSTDELASHQKAAFFGFKNGNPDATVCWGPYAGVVSEAQYKT
                     ISLNHAGAYCDVYSMHSYDWHFDYPRLRKWVFEAAGGKPVWVSESDRGMKSDPESELG
                     DLTWPYEKLKASFVVQSVASSLASGVARHFHFVLPWYMEQNNTVQFGLLRKDGSPRMG
                     YVAYAAAGRLLADAHYLGQWKDASHSTLFAFAFRSKPHGESRDVLVAWAEERNEWKDR
                     GNESVPLHLPDGLNVESAWDFLGRPLPSVIPETATGTPVFLVFSEGALQQLALYKEPL
                     PDRIQYEEPCPVVLQFVAPVEELQQWTANWSEEHYRTALPGKQHWELAAYNFSDHALS
                     GSVALTELPAGWALEQSSWEVQLQPMERLVLPLKAEITADGLPEQEEPFRLVFRGDFG
                     AGQEAHLAVAFRPPAEKTGGEQ"
     gene            complement(15726..>16247)
                     /locus_tag="PKN92_08195"
     CDS             complement(15726..>16247)
                     /locus_tag="PKN92_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ48812.1"
                     /translation="LVLDEIGRGTSTYDGISIAWSVAEYIHDTIKARTLFATHYHELT
                     ELGDKLEHARNISVAVRDWHDSIVFLYRVVDGGADHSYGIQVAKLAGLPGQVLKRAHK
                     ILESLEAGGPATGATESGAQQMFLFAPVEETGPGPLEKELEEVDVDSLTPREAQAFLY
                     HLKHLSNRPRSGT"
BASE COUNT         3973 a         4064 c         4258 g         3952 t
ORIGIN      
        1 gctgctgaga atgggacctt ttccctatgc ccggtgggaa gagattcatc caaatacagt
       61 gcagagtcct ctgtatcttg ttttggatcg cattcaggac gcccataact tcggagcaat
      121 catccgctcc gccgcctctc ttggtgcttc tgcagtaata gtgggggaaa gcagacaggc
      181 tcccgtaaac agtcaagtcg tccgagcctc agccggcgct gtcatgcatc tgcctcttgt
      241 gcaggtctcc gactgtcttc aacaggcgag acagatgaaa gagcagggaa tcagcctcat
      301 tgcaacggct ctgaacgccg aaaaagcggt atgggaatgc tcctttaaac gaccagttgg
      361 cctcatttta ggcaacgaag cagaaggagt agacagggca ttattagagc tctgcgatga
      421 atcttgcaaa ataccgcaaa gcagcggcat agactcctta aatgttgccg ccgctgccgc
      481 cgcaatcctc tacgaaagct ggcgccagaa aaacattgac acaagagtct gatatggcag
      541 tataataaat cgagaaaact aatgaatata agaagcaagc acaggtaaca cggatgacac
      601 tttccattct gatcagcatc atagggttga ttatagcagc agttatatgc ctgctcctgt
      661 ccttatccag agatcattat ctggtcggtg cttttaaaat ggtggcatcc accggttatc
      721 tggttttatg cattgctgca ggggggctcc acagtgctta cggtctggct gttttcatcg
      781 gattaatttt ttcctggtgg ggagaccttt ttctgatttc aaagacccgc agtatttttc
      841 ttctgggctt gctttctttt ttggcgactc atgccactta caccacagca tttatcatgc
      901 agcacccggg aaaatcggca ccggtggcgg cattgtccat tatgcttgtc ccgggaatat
      961 ttgtaggcag gtggctttat ccttatctga aagaaatgcg tgttccggtg cttctttata
     1021 tgaccgccat tacagttatg gtggcatttg cagcgggtgt atttcttacc cgcggttatg
     1081 tgatcacgct ggctggtgcc ggcttgtttt atgtctcgga tctgtttgta gcccgctacc
     1141 actttattgc gcccggcaaa atcaatcagg tcattggtct gcctttatat tacgccgggc
     1201 aaatactcct ttctctgtcc gtcatttatg catacacatg accttatccg gaatgaatgc
     1261 aggtctttcg gtgtatcttt taaaaagcat ataccatcgg agcagcggtg tttccgctcc
     1321 agaaaggagt tttaagaatg gtacaaacag cctccaccat gatgccgcta ggcacgccgg
     1381 caccggattt ttctttgccg gatacccaaa agcagaccgt ttctcttaag aattacgctg
     1441 atgctcccgg acttctggtg atgtttctca gcaaccattg tccctatgtc aaactggtgc
     1501 gcgaagaaat agcccgaatc ggcagagagt acggagcgaa aggtgtggga atcgttgccg
     1561 tgatggcgaa tgatgtccag gcgtacccgg atgatgcccc tgataaaatg gcgttggaag
     1621 tgaaagacgc cggatataca tttccctacc tttatgacga gactcaggaa gtggcaaaag
     1681 cgtatggagc ggcatgcaca cccgactttt tccttttcga tgcacagaag cggctcgtat
     1741 accgcggaca gatggatgat gcgcgccccg gaaataaccg cccggtgaca ggagcggatc
     1801 ttcgggcggc actggacgca cttctggcag gcaagcccgt ttccgatgtg cagcgtccga
     1861 gtcttggatg caacataaaa tggaagccgg ggaacgcgcc tgcttatttc agttcctgaa
     1921 agtggtccca gccgccgctt ttaattgttt tgccgtccat ccataattca gggtcacccg
     1981 cagcagcatg cccgatgccc gtcaggggaa gcggaaacat gctggaaaaa cgcttctgta
     2041 gtgcatcagc ctgctccgga tcaaccgcaa gtgccaattc ataatcttcg ccgccgtata
     2101 aaaaagcatc caaagccggc agaccgcctg cattgccata ggctatctga tcaggcgaaa
     2161 atggaaaatc gccggaattc agatatacgg acacactgct gcactgtgcc atatgcatca
     2221 gatcctgaga cacgccgtcg ctgatatcaa tcatggcgtg caccgcagga tccccggaga
     2281 aaaaaagacc ttcctgcagg cgtggaacag ggcgacgatg cgcctgaagc agggagggag
     2341 gagcgtcttt ttcgtgtagc agactgtgaa gtccctgtgc tgaggcgccc gggtggcctg
     2401 taaccataat cagatcaccg ggcatcatac cccggcggta aagtattcgt ccgccagccc
     2461 ccttgcccgt gacagttaca ttcaaaaaaa gccctgatgc ggaagataca gtgtccccac
     2521 cgatcaggga gcactggctg tctgaaaaaa catgttttaa accggtgtag acatcctgca
     2581 gaaaggagat atctgtatca ggcggcgctg cgaccgttat tagtgcataa agggggatac
     2641 cgcccatggc ggcaatatcg cttaaagcgg tggcagcggc cttatgccct atatcctcgc
     2701 ccgtcgccca gtgtcgttga aagtgcacgc cttcgagaaa agcatcacag gaaaaaagca
     2761 attcctcgcc gccgatccgg acaaccgcac aatcgtcacc gatgcctttt atgacagttt
     2821 tatccggagg aagcccctct gtcagaaagt aaatcagccc gaactctcca agattttgca
     2881 agtcactcac agaccgtatc cttcaagcgc ctgccaggcc gggggcagag catcaatgat
     2941 atcaccagct atcagggcgc gtgttcccag tcgtcgggcg gcaaaatcac ccgccagtgc
     3001 atgcacataa actgccgcac atgctgcatc gaaagctgga atcttctggg caagcagggc
     3061 tcctgcaatg cctgccagta catcccctga accccctgta gccattccgt tatttccgag
     3121 cgggcaaagg gcgactcttc cgtcaggagc ggcaatgatc gtgtcttttc cctttaatgc
     3181 ggttatgacc tgccatcttt gcgcaaattc caatgccaca ggaatacggt ttttctggat
     3241 ataagccgtc ggcagcccgg caagtcggct catctctccc ggatgcgggg ttacaataac
     3301 cggctcttcc ctcaaatgaa atgcgtcttt catgtcgtct ttatgttcag ccaatgcatt
     3361 aagagcatcc gcatcaatga ccagaggaag agtgatctct ctgcacagcc gccggacaca
     3421 ccggacggtt tcctcatgcc gcgaaagtcc cggacccaga agaagcgttg atttatctcc
     3481 gcaaaaagac atcagttcgg aaaaagcggc ttctgaaagg cttccttccg ccgtggaact
     3541 gcacttcttc agcataacct cagtgagaga tgaggcaagg ctgtcaaaaa tggctgcagg
     3601 cacgcaggcg gtgaccagtc cggcaccact tctataggcg ccaaggcagg caagttttac
     3661 cgcacccaca aaccccgtag aacctgctgc cacagcaaga tgcccaaaag atcctttatg
     3721 tccgtccgga ggacgcagcg gaagaaggtg gcgcacttct ttgtgattta ataattgaac
     3781 cattagcaga tcctttgctc tttatattat gtcacactga aaatagaaaa gcacacgttc
     3841 gggaaacttc ggacggagaa ggtcaatgtt gaatctttga aagtcctggc ttacaattca
     3901 ggagcccata ctgcttttat tcggtttgga tgcaggtgtg tttttttatg tggcgctgcg
     3961 cccggattgg tttgggacag taccacagag acaatatcca ggcaaaatga ctctgtctgt
     4021 gtcctggagt ctgcgttttt aatttacatt gtctttctgg ttttaggagc gtcttctgct
     4081 tatgcgccgt attctgcaca ttgatatgga tgctttcttc gcttctgttg aagtggcacg
     4141 aaatcctcag ctccgcggca aagcagtagt tgtcggaggg aattccgaag acagacgcgg
     4201 ggtcgtggcg agcgcttcct atgaagcaag agcatacgga gtgcgctccg ccatgccttt
     4261 aggcagggca aggcagctct gtccccatgc catctttatt cgcggcagtc acggcgttta
     4321 tggagaagtg tcccgcagga tcagagatat cctctattca gtcacgccac tggtacaaat
     4381 ggcatctatt gacgaagcaa atctggatat aacaggctcg atccatcttt ttgacgatga
     4441 ggccgctctt gccctttctc tgaaacagcg catttatgaa aaggaacagt taaccgccac
     4501 cgtcggtatt gcctccaata aaatggtggc aaaaattgct gccaatcagc gcaagccgga
     4561 tggtttcctt gctgtgccga acggcgaaga ggcatctttt ctcgcacctt tatctgtagc
     4621 ggaattgccc ggcgtgggcc cgcgatctcg ggaaatgctg gaggaacgcg ggattctgac
     4681 tgtcgggcag cttgcagcag tgccggaagc agtgctttcc cggctgatgg gcgcgcaggc
     4741 ggcgtcaggt ctgtatgccg ctgcccaggg cgtcggcagc gatattgtcg acccggttcg
     4801 cacgcccaaa tcgatcagcc gtgaaacaac tttttctgaa gaccttcagg attggaagga
     4861 actcgaagtt gtgttattct gccttgccga gcattgtgcg cataccctcc gctctgaagg
     4921 gctggaagca gggaaagtta cgttaaaagt caggtacagc gactttgaaa caaaatcctt
     4981 tggcatcacc cttcctgagc cgacctgtct ggatacccgc attgtagagg ctttgaaact
     5041 acttataccg aaggcacagc agcgccgaac ccgtgtgcgc cttatcgggg tgaacctatc
     5101 gggtctctcc aaagaccagc atcagatgcg gctttttggc cgggaggaca gcgaaaagtg
     5161 ggagcgcctg ctgcatgccg tggacggtat gcgtgaaaaa ttgggcttcg attccatgcg
     5221 cctcggtaat acactgaaag tgaagaaaaa agaagatcct tcgccggaat gacagttcca
     5281 gttattcagg aagcggcggc cagtgcctgc agcagtgcaa tacgtgttgc acgaaaggcc
     5341 tcaacgtccg tattgtagcg gtcaaaatcg agaatatgac ctgctgccag ccgatgcgcc
     5401 tcttcaggat ttttttctgc cagacggcac agcagctcat ggtcaaagat accgtcccgc
     5461 atggcttcaa agcggatgga atcgaggggt ccctctttac ccggatacac aacccagggg
     5521 tctccggcag gaagataggg cggtccgtta tgttcacggg ttgtataaac ccgggggtct
     5581 tcgtttttcc acgcattgta tccccagtgc aggtagcctg tggcgccgct ggtgaagttg
     5641 atccaatgca gaagccgtgt cttaattaac ggaagttcga taaagcgatt ggcatattca
     5701 ccctgaggaa atacgcaggt ataaaaccag acctcatcac cgttcttttg gcgttcctga
     5761 taatgcccga aatcctcatg aaaaaaattc agctgaggta cccagatatc catggagcct
     5821 acgagttctt ttgtatgatt tgcttctatg atacgcaatt ccggggcaaa ctcatgaacc
     5881 agtgccgcca gggctttgta tgtacccata tttcgctggg taggctcatc ggcaagatgc
     5941 tgcatataaa tatccagcca gcctttttcc ttcagatgtg ctgtcagggc cgggtaaaaa
     6001 aagccgtaaa aagtgcgggc ttcttcggaa tcgggtgctg ccgacccgtt ggttacagcc
     6061 ccgtcttgta cactatggat atacgttata aaatccgagt cccagccgcc cgaacgaccg
     6121 ccgatatgcc cgccctcaat acgtccaatg actccttcat caatgaaaat ctgtacccac
     6181 cggtcaaatt tttcaaaatc aactgccagc gcaccttcag cattcacata aaaacgcgaa
     6241 agcgccagtg gggagatcag agcagcattt tgtctgtgtt ccgccatatt ccgggcatag
     6301 cgccgtaaaa gatcgtaata agcggcagaa tccggttcag gattaatttc catatgccgc
     6361 cattgcatgg agaaccagtt ggtgacccac agcctagtgc tgtctactgt gatgggatac
     6421 actaccaact taaggggata agtcgcttcc tgtgtgatgc cgccgctgtc agagcgaacg
     6481 gtaactgatg cctggtacgc tccttccggc acagctggcg gaacatgtac actaatccac
     6541 acaggctgcg cctgctctga tggtacagag acagaagcat cttccaacag cacgtcgggg
     6601 aagtctgccg gtggctggcg gagctgatcc agcgaaggcc tctgtgacgg acggtctaca
     6661 tgaacatagc cgacaaagcg cacagacgcc cccgggatgg ttgttccgct tacagcattt
     6721 gaaaaatcgg tcaccgacgc cgaaagtgta tccagggatg tgtcggaacg gacaacaatt
     6781 tgaaattcac cgtattcgcc ccgggcaaca tgatccacag ctgtaccggc gggctgcggc
     6841 tcggcatccc gaaaaacttt taccagcgga tccacagacc acagttgaag ctcggcaccg
     6901 ggagcgagag aggaaaaaga taagagaatt gctgtcgctg aaagggtata tacaattttt
     6961 ttacagctca tgtttctttc cttagacagt ctgcaagcag gattgtatct gtacgaatga
     7021 tagtgagcct tcactatacc caaagggaaa tagaagttgc aatgcggact ttagtgcgaa
     7081 gtggctttga attgcttcac cgctttgctt acaatgcaat actcgaaacg gattgtgagt
     7141 ataccatact gcgaaataaa ggaaacaaaa tgtacaaaaa tttatttgtg gttggcctgc
     7201 tgtgctgttg cggggcagtg ctgggttgtg tatctgccaa gggtgcaggc aacactgttt
     7261 gcgccgctga aaagacggca gtattgactc cggaagcgcg cctggcgtca ttgaagccgg
     7321 aagaagcgct gtctgctgct gcatttgagg aaaaagtgac tggtgccggc ggcagtgtct
     7381 cttttgcgtt cggcagcgaa cgttcatttg cccagtgcca tgccagtacg ctggtggaga
     7441 ctgccgatgg caaaatcata acagcctggt ttggcggcac ggcggagaag aatcccgatg
     7501 tcggaatctg gtattcagta ttcgatggat cagagtggac tgctcctgtc gccattgcaa
     7561 aggtggaaga gactgctcat tggaatcctg ttcttttcaa agacgagaag gatgtgatac
     7621 acctcttttt caaagttggg gtggatgtgc cgcgctggag tacctggtgg atgtcttctg
     7681 cagacaacgg aaaaacctgg tcggagccgc tggtgcttgt tcccggtgat gtgggcgggc
     7741 gcggtcccgt aaaaaataag gccattattc tgtcggatga caggtggtgt gctccagctt
     7801 ctacagaaga ggggaaatgg gattcttttg ttgatttctc ttccgacggt ggcaaaagct
     7861 ggcagcgttc catggacttt ccccttgatc ggagaaaatt taaaggaaaa ggcaccattc
     7921 agccgacgct ctgggaatcg gcgccgggac acatccatgc attgcttcgg tcaacctgcg
     7981 gtgcaatctt gcgttcggat tccacagata acggcgtgac ctggtgtgat acctatgata
     8041 ccggcatgcc caataacaac agcggcttag atctgattca gctggaagac ggccgtcttc
     8101 ttcttgtgct gaatcccgtt ggcgtcaatt ggggcgcacg cacgcctctg tctcttgcag
     8161 tttccagtga caacggcgac agctggtcta ttcttgcgca tctggaagat gcgccgcagg
     8221 acactgaatt tgagttttcc tatcccgcta ttgtaaaaac cgcaaaggga gtggcaatct
     8281 cctataccta caaacgtgac cgggtcaggt gctggcagat ccctctggaa gccctgtaac
     8341 agaataattt cgcgaaacgg gtgcccgtgc ggcaccgtgt tttaaataaa atcaataagg
     8401 agaataccat gctgaaacaa agattaatac atccggatat tctggaagca ttggcggcag
     8461 cggggcacgg ttcaaaaatc ctgatcaccg acggcaatta tcccgccagc acccggctgg
     8521 gagataatgc cgggctggtc tatctcaact tggcgcccgg catgctttct gttaccgatg
     8581 tcctgaaggt gattctcact gccatcccgg ttgaagaggc cgcagttatg gaaccggaga
     8641 caggtccgga gccttcgatc tttaatgagt ttcgcgcact gctgccggac gtccccctgc
     8701 aggcctataa tcgttttgaa ttttatgaag aagcctccgg ccctgatacc tgcctgcaga
     8761 ttgtgacagg ggaacagcgt atctatgcca atctccttct cacaataggt gtggttgcct
     8821 gattttgcac aaaaaaagcc ggcaatgcgc acagaaggca ttgccggcag gcaaatcaac
     8881 ggggaagggt caggggatca gggtcgcttt cttgatgtag tccaggttgg ggaagtttgt
     8941 tttcagatag gtattaccat ccttggtaat catcccctga ttgggttttt cgctgtattc
     9001 ggaattgatc gccttgacaa tgtccagtcc ttcaatgacc ttgccgaaag gagaaaaacc
     9061 catggtgtcc agacgctggt tattgccggt gttgataaac agctgcgtag tccggctgtt
     9121 gggccctgag gtcgcaaagg tgatataccc ttctttattg ctttctgtga ccggatcatc
     9181 cttgatacgg ttatttttcc atttggcggt aactgccgga tcagcagcaa gcccgaactg
     9241 aacaacaaag ttcggaatta cacgaaaaaa actgctattg tcaaaaaatg cgctttgcac
     9301 taactcataa aatcggtctg ctccaagagg tgcccagtca cggtggcatt caacaacgaa
     9361 gtcaccattg gtacactcga actttacttt aaatacatca ggggttttca caagggtcat
     9421 atcagaatcc ttttgggttg aagaggaggc atcggcatcg gcttccggag cggccttttc
     9481 ctcggcataa atctggttca aacgcaacgc aacctggttg cgggcgatgt gctcactctc
     9541 aggaagccga tccggagcac cgataatact gattgtcagc aacagtgcgg cgctcaaaag
     9601 caaggccgcg gcaatccgtt tttttctggt catggctttt cctttttctg caaaccattg
     9661 gtacggctga aaactatggt gaatacacca aaagagcagg ctgtattcct gcacatgtgt
     9721 cctgttaacg acaaaaagct gccgcgagga tacccctcat tgttcaatga gaaggggaac
     9781 ggctatccct gaactgaaaa gcatcttctg aataacctgc agatgatatt ccgctcatac
     9841 acatcggcgc tgagaatgct caagtaatcc taccattttc atgaaattca gtcaaaaaac
     9901 acatacttag acatcaccga tttgagcgcc tttagggatg actgcatctt ttaagaccac
     9961 tacaattccg tcacggatgg cgaatccgtc tccatcggtg tctttcagcc gcttggaacc
    10021 gcggatcacc acattgtctc ctatccgggc attatgatcg atgatagccc ggctgatctg
    10081 ggtgtttctg cctattccga tgggattttt aagcgttgaa ccggcttcct cgaaataatc
    10141 agcgcccagc actacggagt ttttaatgac agcaccgggc ctcacaatgc ttcgtatccc
    10201 gatgatggaa tcctcaatca gtgcctgctc gatgcgggtg ccgctgcaga gaatggcatt
    10261 tatggtggtc gagcggtcga tccgcactcc gggcagcata cgggcgtgag tataaatcac
    10321 attgttcgga tcgtgaaatt gaaagggagg ccgggaggaa accattgcca tgctgacatc
    10381 aaaataagag cgcacggttc cgatgtcttc ccagaagccg taaaacatgt gtgcaaaaac
    10441 tttgtgactt ttcagcgaat tgggcagcag ttcttttcca aaatcgaccc attcgggatg
    10501 agataaaaga agatctgcaa gcacatgcgc tttaaaaaca taaatgccca tggaagccag
    10561 ataatctttc ccatccgccg ataaaccgaa atcatcaaat accttagaag gggtaatcag
    10621 cgagtctaaa agagcatcat ccgaaggctt ttcaacaaag cgtcggattc cggcatttcg
    10681 gttcactttc ataatgccga agccttgtgc tgcttcacgg cttaccggca gggcggcaac
    10741 ggtgatgtcg gcatcctttt ctatatgtgt tttcagcaga gtcctgtaat ccatgcggta
    10801 cagctgatcg ccggaaagga tcagaaaatg ggaggtacgg ctctggctga tatgatacag
    10861 gtgcttcctg acagcatcgg cagtaccttg aaaccagtcg ccggtctcgt ttgtctgctc
    10921 ggccgccaga atttctacga agccgccgtt aaattcatca aatttatacg cacggttaat
    10981 atgccggttg agcgagtgac tgttaaactg ggtcagaaca ctgatacggt tgatgcctga
    11041 attgatgcaa ttacttagcg gaatgtccac gagccggtag cgtccgcaga gaggtaccgc
    11101 cggtttagat cttacctttg ttaacggata cagccgggtt ccccgcccac cgccaagaac
    11161 aatcgcactc gtcgtgttca atatttcctg ctcagaaaaa gagagtggtt ttgtcatgat
    11221 catacagcct ccggtttaga ataaagccac tgcgtcggta aaaagatttt tggcacgcca
    11281 gaaagagaac aaagcagaaa agacagagag ggaaagaaaa gaaaagaggg ggagcaggtc
    11341 ggataagcag ggcgcggata gccttggcct gctattgcga agagcttaat ttttatcact
    11401 gtcgaccact cccttgaaga cagcttgctt tttcagtgta attacagcac tggagtaaga
    11461 gcacatgctg attgtatcac ctttggcggc gaatactgta acatttgccc tcatcgtgca
    11521 ggactgggcg gagaaacaga gtgaaaggag gagccgatgc gcaggaattt ttcagaaaac
    11581 tgaggggcag cggcggcacc gtgaaaagcg cgtgtcagag agggaggatg gctcggactt
    11641 tttcaatctg ccgaacccgg cgtggcactt cttcaggaac cagccgttcc gaggtgccca
    11701 gtgcaatcca atagggttcc acttccagcg attgctccgg gtggcggaac agatactcta
    11761 tcagcggctg cgttatcatt gcatgcgcaa aagattcgtc cgttgtatag atggcatatt
    11821 tctgggtgaa ttcgctctct tcaaaaagcg cttcatcata accgacaata tcgccgagaa
    11881 cagaaagcat gctccgggga tagatgcgca gaaaaggaca ggtctttccg tgccgtaatg
    11941 cggcgaaact ggagtagtgc catttttttt ccacgcccag cccggtgctg aaagtgtaat
    12001 caaaaaccag gcgagaactg ttcttatcct gtccaatcag aatgttgaag gcataccgtc
    12061 cacgaccgcg gcggtgctgt gctaaaaagg caaagcgctg cggaatggca taacttctgt
    12121 aataatagcg gtatccgtgc ttttttgccg tttcgatgaa agcacggcga atcagatact
    12181 gatagtaaaa gtaaagactt acaccgagta tgagtatgcc ggccagaaaa aaagtgaata
    12241 gaatatttcc aagtacatca tccaaggcga ctatccaaag gcgagggggt cacaggtaac
    12301 cagctgtctt gttatagcac atcggcaatg gcattacata aagcaggcat gtttttttct
    12361 gtcattccgg caacactgat ccttccggaa ccgacaatat aaattgcgta gcgtgtccga
    12421 agcagttcca cctgatcttt attcagtcct gaaaaggaaa acatgccatg ctgcctgcgg
    12481 ataaaggaga agtcctgttt aacacccaga ctctgcagtg tatccacaaa aagagcgcgc
    12541 atttcattaa tacgtttgcg catgcctgcg acttcctctt cccacatgcc acgcaaaata
    12601 tcactttcga gaatggtggt aactaccagg cttccatggg cagggggatt ggaataattg
    12661 gcgcgtatcg ccttttccac ctgactgaag gcacgggctc cgctgtctgc atcggcagcg
    12721 acaagggtca gtgcgcctac ccgatcctgg taaaggccga aattctttga ataggaactg
    12781 cagataagca cttcgggcag ctgttcagaa agaaggcgta caccgacggc atcctcatcc
    12841 agtccggcgc caaaaccctg ataagcaaag tcaatcagcg gaagaagaga tttttccctg
    12901 attaactcgg acatggtaag ccactgttcc tgacttaaat caacaccgct cggattgtgg
    12961 cagcaggcat gcagcagcag cacatctccc ggggcgcttt ccccgagact tgccagcatt
    13021 ccgtcaaagt cgatgtcctt gctggcagga tcataatagg tgtaggcttt tgtttccagc
    13081 cctgcagcct cgaagatagc gccgtggttt gcccaggtgg gattactcag ccagacacga
    13141 cccttcggat tccaccggta caggaagtca cctgcaacgc gtaaggcacc tgtgccgcct
    13201 ggtgtgtttg ctgtataggc gcgaccgttt tcagtcaccg aatggctttt gccgaaaaga
    13261 agtgtcctgg tggcgttggc aaaagcaggg gagccggtga tggaaagata gctttttgtt
    13321 tccgcttctt ccacaagaat gcgctctgcc cgtttaacag attccagcac cggagtattg
    13381 cctgccgcat ctttatagac accgacgccc agattaatct tattggggtt gctgtctttt
    13441 ttgaatgctt ctgtcagtcc caggatgctg tcggcaggtg ccatttgcag tgtttcaaac
    13501 atggggtata tacctctccg aattgcataa aggaatggct gatacagtaa ggaactgcag
    13561 aagagcggct ccttcgacgg ggaacgtgca tatcataaaa taaaaaggga cgtgtgagca
    13621 aaagaagact cctcagggcc tgaaaatcca aaggatctgg aatcatcagt cctgtttcag
    13681 atataacgta ttatgttccg gcaaaagcct ttatgagcga cggggccgga attgctgttg
    13741 actgacgtgt ctgtttcatt tcagacaagg agattgtttt catgataaaa cattgcttta
    13801 tgctggccgt tgtcctgata ggatctatga ttgcttcagc ggatcagttt gtttttacct
    13861 ctgaagagcc catagacctg acagcgcctc cctctgcggc tgtatgtcat tttcttcagg
    13921 aggacttcag cccttttcgt gaggcgtttg ttcaaaacgg gaaggcagtg cttttaccgg
    13981 gggagctgcc tgtcggctgg tataaggttg atttcttaaa tgccgagggt aaacttctgg
    14041 attggacgtc tatcgctgtt ttaatacccc tgctgaaaag accttccccg cagactccgg
    14101 tcgcaattga tgtggctctt tcgtgggtgc cgccgcccgg agaagcaagc tggcgttcag
    14161 tggcacgcat ggcggcgctt gcgggtgtgt ccatggtacg tgaccgtata ctttggcgcg
    14221 accttcagcc ggagcccggg ccactcgttg cctatacaaa atatgacgat acgtctgtaa
    14281 ttcaaaaaga tgccggtctc gacattcttc aggtgttcca cgatgtgccc tactggatgt
    14341 tgggctatcc acagggtaaa agacatatcc ctgaagacct tcgcctggtc tggcggttct
    14401 gccgcgaagc ttccgcaaga tataaagata cagttcaggc atggcagccc tggaacgagg
    14461 ggaatacacg cactttcggc ggccacagca ccgatgaact ggcgtcccac cagaaagctg
    14521 cttttttcgg atttaaaaac ggcaatcccg atgctaccgt atgctgggga ccttatgcag
    14581 gtgttgtttc cgaggcacag tataaaacaa tctcacttaa tcacgctggc gcatactgtg
    14641 atgtttactc gatgcacagc tatgactggc attttgatta tccccgcctc cggaaatggg
    14701 tttttgaagc tgccggcggg aagcctgtgt gggtgagtga atctgatcgg ggcatgaagt
    14761 cggatccgga atcagaactg ggtgatttaa cctggcctta cgaaaaactg aaagcgtctt
    14821 ttgtcgttca gtccgttgct tccagtctcg caagcggcgt ggcgcgccat tttcattttg
    14881 tgctcccctg gtatatggaa cagaacaaca cagtgcaatt cggactgctc cgaaaagatg
    14941 gatcgccgcg tatgggctat gtggcatatg ctgctgcagg gcgtcttctt gcggatgcac
    15001 actatcttgg ccaatggaaa gatgcttctc actcaacact ttttgctttc gcatttcgct
    15061 caaagcctca tggggagagc agggatgtgc ttgtcgcctg ggcagaagag agaaacgaat
    15121 ggaaagaccg cggcaatgaa agcgtgccgc tgcatttgcc ggatggtctg aatgtggaaa
    15181 gcgcatggga ttttctggga aggcctctgc cttcagtaat ccctgaaaca gcgacgggaa
    15241 ctcctgtttt tcttgtgttt tctgaaggag cattgcagca actggcgctg tataaagagc
    15301 cgttgcctga caggattcaa tatgaagagc cttgtcccgt cgtgcttcag tttgtcgcac
    15361 cggtagagga gcttcagcaa tggacggcta actggtctga agaacactat cgcacggcgc
    15421 ttccgggaaa gcagcattgg gaactggcag catataattt cagcgatcat gctctttccg
    15481 gcagtgttgc actcactgaa ctgcccgccg gctgggcatt ggagcaaagc agctgggaag
    15541 tgcagctgca gcccatggag cgtcttgtgc ttcctctgaa ggctgagatt acagcagacg
    15601 ggctgccgga gcaggaagag cctttccggc tggtatttcg tggtgatttt ggtgcggggc
    15661 aggaagcaca tcttgcggtt gctttccgtc cgcctgcaga aaaaacagga ggggagcagt
    15721 aaagatcagg ttccgctgcg cggtcggttg ctcaaatgct tcaggtggta cagaaacgcc
    15781 tgggcctccc ggggcgtgag tgaatccaca tccacctctt ccaactcttt ttccagaggc
    15841 ccgggcccgg tttcttcaac aggagcaaag aggaacattt gctgcgcccc ggactctgtg
    15901 gcacctgtcg ccggcccgcc cgcttccagc gattcgagga ttttgtgtgc ccgcttcagg
    15961 acctgtccgg ggagtccggc aagcttcgcc acctgaatgc catagctgtg atcggcgcca
    16021 ccgtctacta ctctgtacag aaagacgatg gaatcgtgcc agtcgcgcac ggcgacgctg
    16081 atattgcgcg catgctcaag tttgtccccc aactctgtca gttcatggta atgggtagcg
    16141 aagagggtgc gcgcttttat ggtgtcgtgg atatattccg ctacggacca ggcaatactg
    16201 atgccgtcat aagtgctggt gcctctgcct atttcatcga gaacaag
//
LOCUS       DAOPWB010000075        15869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3764_length_15869_cov_5.455482, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000075 DAOPWB010000000
VERSION     DAOPWB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 15869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15869
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_15869_cov_5.455482"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..441
                     /locus_tag="PKN92_08200"
     CDS             91..441
                     /locus_tag="PKN92_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNZ48813.1"
                     /translation="MLTIDQQTKDAVAVLTASGVLDQAEIHKLREAVARNIRKHQFHL
                     VLNLRHVGMIEYPSFGVLLELLSELQQYGGGLKLACANLHTQRMLRLMGLCRVLMCFD
                     TEAEAIRQYQKEAA"
     gene            446..1432
                     /gene="rsmH"
                     /locus_tag="PKN92_08205"
     CDS             446..1432
                     /gene="rsmH"
                     /locus_tag="PKN92_08205"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNZ48814.1"
                     /translation="MKMPAGRQQSGDKRMSDFPAPHAPVMLEETISFLQLRSSGIYVD
                     MTAGAGGHSEQIARHLGQGRLIAIDRDKEAVALTRKRLSPYPAATVVRANYSELKTIL
                     EAQGCPAVDGILLDAGVSSMQIDRAERGFSYLKEGPLDMRMDSDAPLDAAAWLQCVSP
                     RDLEQVLKEFGDVRPVRRIARHICKRRDQGQLKSTSDLAEAIRDALNHVVPNPVELRQ
                     VFQAVRMAVNDELNHLKTGLEHGAESLSPGGRLVVISFHSGEDGLVKELFKRLSRQQT
                     ELRPDGRILKRVPALYKTVTARPVLPTPEEIQRNPRAKSAKVRVLERLQSAE"
     gene            1453..1998
                     /locus_tag="PKN92_08210"
     CDS             1453..1998
                     /locus_tag="PKN92_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48815.1"
                     /translation="MKIFCKVGQDQNIWAWIFRLFCLAGLFSLLFIDAYFNIQRRNND
                     YEIGRLGARRRALEDELTQQNSLLSQVKPKEFMEKKAEELGLQNPDVYQFQVVQYREV
                     PRKAPILRLDNLQRPLEGLNPPVKRIEMANRPELEAAPAIPVQTVPAVEAVELKTVPA
                     PAEVEDPELSFMSVGDMLASL"
     gene            2063..4276
                     /locus_tag="PKN92_08215"
     CDS             2063..4276
                     /locus_tag="PKN92_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNZ48816.1"
                     /translation="MAFLRRFPLHPDPDLKRKAMQQHQLRACFAGAGLLVLGTFIIGM
                     LVYSNFHPQENLASEDETHIGRFFIKNQRGEIFDRNGTVLAVNKSAPTLQVDPRKVED
                     ADALATFLSKHIDMSWEDIFARLAKLDESGQVRKMNTIKRWIEKPSREELEKIIASEP
                     SLSLRDEPLRHYRYGDSAAHLLGFVNRAGEASEGLELAFDKYLHPRAGEIRARTDNGR
                     QLLPSGLLSYQEPEGGDKVQITVDINMQQTLERCLDKRMEEVGAKAAMGILMDPHTGA
                     IFALANRPAFDPNHYDLYDPALRKNRVALDRFEPGSAFKIVVSSAALENHLVYPEEMI
                     DCENGGFNPYGHYIKDFYRLGVIPFSYAFAKSSNVAMIKLGKRLGPERMDEWIRLYGF
                     GEKTSRDFKHESTGIYHAREKWSRLSMGSLPMGQEIAVTSLQLARAFAVIASGGLLVE
                     PYFVEQAVNRYGEVTYKHDPLPPKRVLSPDTAKTMVELCHQVVLHGTGTTANIPEFRV
                     GGKTGTAQMAKSNGRGYDPDRYTAVFAGFAPVASPSIVGVIVVQEPAIRQRWGGFVCG
                     PVFQEVVKDALIRLGTPEDPVVNPDNPRMEPVKKTAPKEQAPAQSKDKERTLIADLIA
                     EEDADTVSPPPAPEDIDENFQALITPLDGISLVARPMAAKGDEKAMPDLTGKTKSQVR
                     MELQRMGIVLDAQGVGWAVEQEFAPGLSVGDVKVCSIRFSDKMPPMEKGNPDDAG"
     gene            4263..5753
                     /locus_tag="PKN92_08220"
     CDS             4263..5753
                     /locus_tag="PKN92_08220"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNZ48817.1"
                     /translation="MTQDELSTLLDIPPIHRELEINSVTEDSRKVHPCSLFIARAGQK
                     SDGHSYMEQAVANGAAAVLGNRKDIADAFGKPYIFYEHPRRAAGLIAHALEGNPSENL
                     CVIGVTGTNGKTSTCCLIQQILNHSGFLCANFGTLGYSLPEEEVPAAHTTPFGEDLAQ
                     LFSRAKQQGCTHVVMEVSSHALEQERVAGIHFNIGAFTNLTQDHLDYHQSMDAYLEAK
                     LLLFHKLHAVFQDPQGARPCFSAVNREDPSAARFIEAFPEACISYGKGGTIHAKEVVL
                     DIHGAQFTLVTPEGSAAVTLSLVGRHNVQNALCAAAICHGAGIPLKAIAAGLASLTSI
                     PGRMEAVGEGFPFQVVVDYAHTDDGLRNALNAARAVCKRKVIVVFGCGGDRDKSKRPK
                     MGAVAAELADLAIITSDNPRTEDPFLILLDVEIGLQKRQKYKGDDYLVIESRQEAIEK
                     AVSMAAPGDLILIAGKGHEDYQIIGTERRHFDDREAARAALKALSL"
     gene            5750..7108
                     /gene="murF"
                     /locus_tag="PKN92_08225"
     CDS             5750..7108
                     /gene="murF"
                     /locus_tag="PKN92_08225"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNZ48818.1"
                     /translation="MSMHYTLQEAGAMMGLSLSGSMQDAPVITGVSTDTRTLQKGDLF
                     FALNGENFRGDDFVAAAFQKGAVAAVTDGSGYKDPFFQTSNALEALQRLAGAHRDRCP
                     ARIIGITGSCGKTTAKDMLASVLSSEYVVEKTQGNLNNDIGCPLSLLRLSQATHFGII
                     EMGANHIGEIRALCGLAKPDEGVVTLVAPAHLEGFGSLEGVAAAKAELMEALPEKGCF
                     YVNMDDPLCRAMGERFRGETVRFGTGGDVYLKELRFDDEGEMVLKVHPVGTLRLPLRI
                     RAHVSHVLLAVAAGLRHGIISLEAPLRDACKKAARFKEALFQGVHVLDDSYNANPASM
                     KASLEALRDYPGQRRIAVLGDMFELGKSAEALHADIGRFAASLSIDFLLSLGGYGGIM
                     AEAAHRAGLDQAFACASHQEAAAWLQNNTAAGDAVLLKGSRGMKMETVFEQWKSIAPA
                     GQ"
     gene            7121..8605
                     /gene="mraY"
                     /locus_tag="PKN92_08230"
     CDS             7121..8605
                     /gene="mraY"
                     /locus_tag="PKN92_08230"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNZ48819.1"
                     /translation="MAYWLSVLLKDEVGFTNVLSYHSVRAGGAAFTAFFLSILLGPAI
                     IRRLRQLKIGQYIREEHVESLHELHKGKAGTPTMGGLMIIVSTLAALLLWGRLSNRLL
                     WVSMIILLVMGALGFMDDFIKLKRKHNAGLSARAKFAGQILTGLLLGIYLVNNPITVS
                     ESYVLHRDVINWPLLESMLAGAHERSQTPDVKKICSMLSPECRSIIRGNVNEAQITDE
                     EQQTVLKELNLALRSTELYEEALWHDIVKNPEARRLLQSSPEKMSERDLIRFNRLLLE
                     QSFSGMIAESVPNLHTKLGIPGFKELFIPLGFFYIFFVTLVMVSITNAVNLTDGLDGL
                     AAGVSIISILAYAAIAYIISRADWSRYLFLTYVPEASELFVFGAALLGSGLGFLWFNG
                     HPAEVFMGDTGSLALGGAIGALALLTKQELLLPVVAGLFVLEAASVVIQVFSFKLTGK
                     RVFRMSPLHHHFELCGWKETKVTLRFWILAFLFALLSLGALKLR"
     gene            8622..9995
                     /gene="murD"
                     /locus_tag="PKN92_08235"
     CDS             8622..9995
                     /gene="murD"
                     /locus_tag="PKN92_08235"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNZ48820.1"
                     /translation="MDLTGKHILLVGLGRSSVAAAQLIMEKGGMPFISERNDGQSLEN
                     WLEICRTRGIPVETAGHTSPLFQTADLVVINPGVPYNAPCLDIPKEKGIPVVGELEFA
                     SWFNHSTVIAVTGTNGKTTATSLLYTMIRTCGYSVALAGNTHTAWSEVTAKEEQPEYT
                     VLEVSSYQMETADTFHPKVAVILNLTPDHLGRHGTMECYGATKARIFQRMQSGDTVIL
                     NKDYAALRALPLPFQAETVWFGKARDGDKTGWSYDTAHFYQDGEIAADAKDSPLPGLH
                     NKENITAVLAAAHALGLDWGGVLQGLRQFAGVEHRIEYVTAINGIAFFNDSKATNIDS
                     LRVALESFVQPVVLIAGGEGKGSSYEPLRSLIRDHVSTLITIGEEAPALEACFQKDVF
                     ICRGTTMKDAVYKAYTAASPGSVVLLSPACASFDWYSNYEERGGDFKNCVHALKEDLH
                     TGEEKGK"
     gene            9992..11167
                     /locus_tag="PKN92_08240"
     CDS             9992..11167
                     /locus_tag="PKN92_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNZ48821.1"
                     /translation="MKKETNTLIMAVLALVLLGTLMMYSIDAVRGPKSMILYKHLAFL
                     GLGLIGFFFTMILDYHVLGSPAVRKAFLIGTLILLACTFIPGLRLSMGGAYRWIGWKQ
                     LSFQPAELARFTLVLMLAVRLSKQQDNMSYWTTSLVKPGFYLGLFAGIVVLQKDLGIP
                     VVMSVATFIIIWTAGARIAHLGLAGAVGITAIIPLIAMEGYRATRIKSFMDPWAARDS
                     HGYQLIQSLSSFYQGGVWGRGVGFGEQKLGYLPAAHTDFIFAMIGEELGLVGTIVVVL
                     LFTAILIAGIRTVVRAPDLFGSLLAVGITTLIVFQAFFVMLVNLGWLPTKGLPLPFVS
                     YGGSALMTNLWMAGVLINIASQASEKEKTARSSVFTHSRLKYLFRIFLPKKPATVRI"
     gene            complement(11245..12528)
                     /locus_tag="PKN92_08245"
     CDS             complement(11245..12528)
                     /locus_tag="PKN92_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNZ48822.1"
                     /translation="MNYSFSSYGRGSVEPSPVNRMMAAFATDFRDAKDINLGVGYVNE
                     KTIPTELLAEVVKEIASCPQDFRQAFNYGGPQGAPGLIQALRDYYVEEAIGGMTEEVM
                     SAQEVVIGPSGATSILEALADIFEPGIVITADPMYYIYCNHLERKGFQVITVPEEKEG
                     LNPASVRRKLESLGDQAQRLSFFYVVTVNNPSGNVLCNAYRRGLAQLAKEWSARLGRL
                     CPVFFDQAYEWLIHDPEAEPPRSALVDTPDAPVYEIGTLSKVLAPAMRIGFMLGAPGV
                     LMQALVQKTSDVGFSAPLLNQEIAAVMLCRYMHDQVARVKAGYRAKSEIVGQAIREML
                     GPWLEEVCGGTAGFYYYLTFKTSAPTVRPLFSAILPEPQALKNMTVLPEILIRGSFIS
                     RENSVYIPGEASRMPECGSCAFPMVLKRRIRSWRR"
     gene            complement(12747..13112)
                     /gene="rplS"
                     /locus_tag="PKN92_08250"
     CDS             complement(12747..13112)
                     /gene="rplS"
                     /locus_tag="PKN92_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNZ48823.1"
                     /translation="MNLVEEYASQYCKESQALPKFKVGDNVRVHFRIVEGEKERVQVY
                     EGVVISHKGKDSPTATFTVRRVAFNEGVERVFPLHSPRIEKVEVTREGRVRRAKLYYL
                     RDRSGKAARVKARRRNVQA"
     gene            complement(13109..13861)
                     /gene="trmD"
                     /locus_tag="PKN92_08255"
     CDS             complement(13109..13861)
                     /gene="trmD"
                     /locus_tag="PKN92_08255"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNZ48824.1"
                     /translation="MRIDILTLFPEMLEPALRIGILGRAIAKDILTVNLVNPRDFAHD
                     RHRTVDDAPYGGGAGMVMTCPPVFEAVESLISKNSLEHVVLLSPRGRKLDQNLVREMA
                     GWKDMVLLCARYEGMDERIAETLVTEEISIGDYVLSGGEAAALVLVEAVSRMAPGVVG
                     NWESVDTDSFYHGILGYPQYTRPPVFRGKEVPRVLLEGNHEAIRKYRRREALRATWIR
                     RPELLTNLSKEDLELLAEIGVHLPPSNKENLP"
     gene            complement(13889..14431)
                     /locus_tag="PKN92_08260"
     CDS             complement(13889..14431)
                     /locus_tag="PKN92_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48825.1"
                     /translation="MNQPERIVIGQVLAVEAARRSLRIQLKHKYLQSLDDCDRIWLTT
                     VDGVPLRAKIETCERNGTSARVALTAGICRDEMQALHRAEVAVDSRDCKKVSADMPDL
                     EELEGFKVIAPGGRLLGVVFETIDTPAGGVIRLLTGEGRTAALPVIAEVIASVDINKK
                     RIAVVDPEPFLVMDDALGNE"
     gene            complement(14428..14679)
                     /locus_tag="PKN92_08265"
     CDS             complement(14428..14679)
                     /locus_tag="PKN92_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNZ48826.1"
                     /translation="MSAKDLVEFVAKKLVTCPDSVSVRLIESDTGETVQLTVDEADMG
                     RIIGKNGRTIKALRTLLYAAGTKSEKNLGLEITNQTEAQ"
     gene            complement(14679..15116)
                     /gene="rpsP"
                     /locus_tag="PKN92_08270"
     CDS             complement(14679..15116)
                     /gene="rpsP"
                     /locus_tag="PKN92_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNZ48827.1"
                     /translation="MATVIRMKRGGSKGQPYYRIVVQDSRTRTRGRELDILGFYHPTA
                     RPEPISEINIHRALDWLGHGAQMSDTARSVMSKLGVLKHFHDGTRPEEAVATRKGEAV
                     VDKGYNAPPPPKAAKAPVEEKAEEAAVEESVEAVAEETPAEEA"
     gene            complement(15242..>15869)
                     /locus_tag="PKN92_08275"
     CDS             complement(15242..>15869)
                     /locus_tag="PKN92_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNZ48828.1"
                     /translation="GVILTQMDGDARGGAAISLIDVTGCPIKFVGTGEKLEALEPFHP
                     QRMADQILGMGDIVSLVEKAQQVVDQEKALHFQKKVRKGDWDLEDFLDHMRQLKKMGS
                     MTELLQKIPGISKMMPQGMDFAEDEMKHTEAIILSMTLKERRNPNIINGSRRRRIADG
                     SGLSVQDVNALLKEFQKMKVMMKQAMKGGKGRKMGRMPGGPMPPMPGL"
BASE COUNT         3704 a         4229 c         4178 g         3758 t
ORIGIN      
        1 gcttacaaaa tatggatgac tataaagtac gggctagcga gattttttcg ggtcacagcg
       61 catcgacaaa cattcaggag gaagcgaaac atgttaacga ttgatcaaca gaccaaagat
      121 gcagtggcag tgctgactgc ttcgggcgtg ctcgaccagg ctgaaattca caaactccgt
      181 gaggccgttg cccgcaacat ccgcaaacac cagtttcatc tggtgctgaa tctgcggcac
      241 gtcggcatga ttgaataccc gagcttcggc gttttgctgg aactgctcag tgaactccag
      301 cagtatggcg gcggtctgaa acttgcctgc gccaatctcc atacgcagcg catgctgcgt
      361 ctcatgggtc tctgccgggt tctcatgtgt ttcgatacgg aagcggaggc aatccggcag
      421 tatcagaaag aagcagcgta atcatatgaa gatgccggct gggcggcagc agtcgggaga
      481 caagcgcatg tccgatttcc ccgctcctca cgcaccggtg atgcttgaag agacaatctc
      541 ctttcttcag ctgcgcagca gtggcattta tgtggatatg acggcgggtg ccggcggtca
      601 ttcggaacaa atcgcccgcc atctgggtca aggacggctt atagctattg accgcgacaa
      661 agaagccgtt gccctgacac gcaagcggct gagcccctat ccggcagcca ctgtggtccg
      721 ggcaaattac agtgagttga agaccattct ggaggcgcag ggatgccccg ctgttgacgg
      781 cattcttctt gatgccggtg tttcgagcat gcagatagac cgggcggaac ggggattttc
      841 ctatttgaaa gagggcccgc tggacatgcg tatggactct gatgcgcctc ttgatgctgc
      901 tgcctggctg cagtgtgtca gtccccggga tcttgagcag gtgctgaaag agtttgggga
      961 tgtccggcct gtgcgccgta ttgcacggca catctgcaag cgccgtgatc aggggcagct
     1021 gaaaagcaca tcagatctgg cagaagcgat ccgggatgca ttaaaccatg ttgttcccaa
     1081 tcctgtagag cttcgccagg tgtttcaggc cgtccgcatg gcggtcaatg acgagttgaa
     1141 tcatttaaaa accggactgg agcatggggc ggaatccttg tctccaggcg gcaggctggt
     1201 agtcatttct tttcacagcg gtgaagacgg cttagtaaaa gagctgttta aaaggctcag
     1261 cagacagcag acagaattgc gtccggacgg gcgtattctg aaacgtgttc cagccctgta
     1321 taaaacagtt actgcccgtc ctgtcctgcc aaccccggaa gaaattcagc gtaatcctcg
     1381 cgctaaaagc gcaaaagtca gagtactgga gcggcttcaa tccgcggagt gaaggtatta
     1441 aggagtgaga aaatgaagat tttctgcaaa gtcggtcaag atcagaacat ttgggcatgg
     1501 atttttcggc tcttctgcct cgcaggtctg ttttctcttc tcttcattga cgcctacttc
     1561 aatattcaac gacgtaataa tgattatgaa atcgggcggc tgggcgccag gcggcgcgca
     1621 ctggaagacg agctgacaca acagaactcg ttgctgtcac aggtaaagcc caaagaattt
     1681 atggaaaaaa aagccgagga actgggattg cagaatccgg acgtgtacca gtttcaggtg
     1741 gttcaatatc gtgaagtccc ccgaaaagcg cccattctcc ggttggataa tctgcagagg
     1801 cctcttgaag gactgaatcc tcccgttaaa cggatagaga tggcgaatcg tcctgaactg
     1861 gaagcagcac ctgctatccc cgtgcagacc gtaccggctg ttgaagccgt tgaacttaag
     1921 accgttccag cgccggctga ggtagaggat ccggagttgt ctttcatgtc ggtcggtgac
     1981 atgctggcat cattataaac ggcggcggaa cggagagaat tgcaggctct gccgttcctg
     2041 aaaaaaaggg aggtaccatc tcatggcatt tctgcggcgg tttccactgc accctgatcc
     2101 tgatttaaaa cggaaggcca tgcagcagca tcagctacgc gcctgtttcg ccggtgccgg
     2161 acttctcgtt ctcggtactt tcattatcgg tatgctggta tattccaact ttcatcctca
     2221 ggaaaacctg gcttcagagg atgaaacaca tatcgggcgt tttttcatta aaaatcagcg
     2281 cggcgagatt ttcgaccgca acggaacagt gcttgcggta aacaagtcag caccgacact
     2341 gcaggtcgat ccgcggaagg tggaagatgc ggatgcactt gcaacctttc tcagcaagca
     2401 cattgatatg tcctgggaag atatttttgc acgccttgcc aaactggacg aatccggcca
     2461 ggtgcgcaaa atgaacacca tcaagcgctg gatagaaaaa ccttccagag aagaacttga
     2521 aaagattatt gcttccgaac cttctctctc gcttcgagac gaacccctgc ggcactatcg
     2581 ctacggcgac agcgcagcgc atcttttagg ctttgtaaat cgcgccgggg aagccagcga
     2641 agggctggaa ctggcctttg acaaatatct gcacccgcgc gccggagaaa tccgcgccag
     2701 aacagacaac ggcagacagc tgctgccttc ggggcttttg tcttatcagg agcctgaagg
     2761 gggagacaag gtacagatta ctgtagacat caacatgcag cagacgcttg agcgctgtct
     2821 ggacaaacgc atggaagaag tgggcgcaaa ggcagcgatg ggcatcctga tggatcctca
     2881 tacgggcgcc atctttgccc ttgccaaccg ccccgctttt gatccgaacc attacgatct
     2941 gtatgaccct gcactgcgga aaaatcgtgt cgctctggat cgttttgaac cgggctctgc
     3001 tttcaaaatc gtcgtttcct ccgcggcgct tgaaaatcat ctcgtctatc cggaagaaat
     3061 gattgactgc gagaacggcg gattcaatcc ttacgggcat tatataaaag atttctacag
     3121 gctgggcgta atccccttct cttatgcctt tgccaaatcc agcaatgtcg ccatgatcaa
     3181 actgggcaaa cggctcggtc cggaacgcat ggacgaatgg attcgccttt acggattcgg
     3241 cgaaaaaacg tcccgtgatt ttaagcatga aagcactggg atttaccacg cccgtgagaa
     3301 atggtctcgt ctgtccatgg gctccctgcc catgggccag gaaattgccg taacctcatt
     3361 acaactggcg cgtgcctttg cggtgattgc cagtggcggc cttctggtgg aaccctattt
     3421 tgttgaacag gcggtgaacc ggtacgggga ggtgacctat aagcatgatc cccttccacc
     3481 gaaacgcgtt ctgtctccgg acacagcaaa gaccatggtg gaactctgcc accaggtcgt
     3541 gctgcatggc acaggtacga ccgcaaatat ccctgaattc cgcgtgggcg gcaagaccgg
     3601 cacagcacaa atggcaaaaa gcaacggacg cggatatgat cctgatcgtt acaccgctgt
     3661 ctttgcaggt tttgcacctg tagcgtctcc cagtattgtg ggtgtcatcg tggttcagga
     3721 accggcaatc cgtcagcgct ggggcggctt tgtctgcggg cctgttttcc aggaagtggt
     3781 aaaagatgcg ctaatccgtc tgggaacgcc ggaagatccc gtcgttaacc cggacaaccc
     3841 ccgtatggaa cccgtcaaaa agacagcacc gaaagagcag gcacctgcgc agagcaaaga
     3901 caaagagaga acacttattg ccgatcttat cgccgaagaa gatgccgaca ccgtctctcc
     3961 tccgcctgca ccggaagaca tcgatgaaaa ctttcaggca ttgatcacac cgcttgacgg
     4021 catttctctg gttgcgcggc ccatggctgc caaaggtgat gagaaagcaa tgcctgatct
     4081 gacgggaaaa acaaagtctc aggtcagaat ggaactgcag cggatgggta ttgttcttga
     4141 tgcacagggt gtcggctggg ccgtggaaca ggaatttgct ccgggattat cggtaggcga
     4201 tgtgaaggtc tgcagtatcc gcttttccga caaaatgccg cccatggaaa aagggaaccc
     4261 cgatgacgca ggatgaactg agcacactgc tggatattcc gccgattcac cgggaattgg
     4321 aaatcaattc tgttacggaa gattctagaa aagtgcatcc ctgctcgttg tttattgcaa
     4381 gagcggggca gaaatctgac ggacattcct atatggaaca ggctgttgcc aacggtgcag
     4441 cagccgttct gggtaatcgt aaagacatcg ctgacgcttt cgggaaaccc tacatttttt
     4501 atgagcatcc ccgacgggca gcaggactca ttgcgcatgc tctggaaggc aacccttccg
     4561 aaaatctgtg tgtgatcggc gttacgggca ctaacggaaa aacaagcacc tgctgcctga
     4621 tacagcagat cctgaatcat tccggatttt tgtgcgccaa cttcggcaca ctcggataca
     4681 gcctgccgga agaagaagtg cccgcagcac atacgacccc ttttggcgaa gacctggctc
     4741 aacttttttc ccgggcaaaa cagcagggct gcacacatgt cgtcatggaa gtcagctctc
     4801 acgccctgga gcaggaacgt gtcgcaggca ttcatttcaa tataggcgcc tttacgaacc
     4861 tcacacagga ccatctggac taccatcagt ccatggacgc ttatcttgaa gccaaattgc
     4921 tgctgtttca caaactgcat gctgtttttc aggatccgca gggcgcccgt ccctgctttt
     4981 cagccgtcaa ccgggaagac ccttcggcag cgcgttttat tgaggctttt cctgaagcct
     5041 gcatctccta tgggaaaggc ggaaccattc atgcaaaaga ggtagttctc gatattcacg
     5101 gcgcacaatt tacactggtc acccccgaag gctctgcagc ggtcacgctt tctctcgtcg
     5161 gcaggcataa tgtgcagaat gcactgtgcg ctgcagcgat ctgtcacgga gcaggcatac
     5221 cgctgaaagc aatagccgcc ggacttgctt ccctgacatc gatcccgggg cgcatggaag
     5281 ctgtcggcga aggttttcct tttcaggttg tcgtcgatta cgctcacacc gatgacggat
     5341 tgcgcaacgc actcaacgcc gcccgggctg tctgcaagag aaaagtcatt gtcgtattcg
     5401 gctgcggagg cgacagggat aaaagcaaac gtccgaaaat gggtgctgtt gcggcggagc
     5461 tcgccgactt ggccattatc acctcggaca acccccgaac agaagatcct ttcctgatcc
     5521 tgctggatgt ggagattgga ctgcagaaac ggcagaaata taaaggcgat gactatctgg
     5581 tcattgaatc ccgtcaggaa gcaatagaaa aagctgtatc aatggcagca ccgggcgatc
     5641 ttatactgat cgccggcaag ggtcatgaag attaccagat tatcggaacc gagcggcgac
     5701 attttgacga ccgggaagca gcgcgcgccg ctctgaaagc tctttcatta tgagcatgca
     5761 ttatacactg caggaagccg gtgccatgat ggggctttct ttatcgggaa gcatgcagga
     5821 cgctccggtg atcacaggcg tgtccacaga tacccggaca ctgcagaaag gcgacctctt
     5881 tttcgcactg aatggagaaa acttccgcgg ggatgatttc gtggcggctg cttttcaaaa
     5941 aggcgctgtt gcggctgtaa cagacggatc gggctataaa gatccttttt ttcagacatc
     6001 caacgcactg gaagcactgc agcggcttgc aggcgcacat agagaccgct gccccgcacg
     6061 cattatcggc atcacaggct cctgcggaaa aactacggca aaagacatgc tcgcctctgt
     6121 actctcttct gaatatgtgg tggaaaaaac acaaggcaac cttaacaacg atatcggatg
     6181 ccctctttcg ctgctgcgcc tgtctcaggc aactcacttc ggcattatag aaatgggcgc
     6241 caaccatatc ggcgaaatcc gggcgctctg cgggcttgca aagcctgatg agggcgtagt
     6301 tacacttgtg gcgcctgccc atcttgaagg cttcggatca ctggaaggcg tggcagcagc
     6361 caaagcggag ctgatggaag cacttcctga aaagggatgt ttttatgtga atatggatga
     6421 cccgctctgc agagcgatgg gcgaacgttt tcggggagaa acggtacgtt tcggtacggg
     6481 cggggatgtg tatctgaaag aacttcggtt tgatgatgag ggtgaaatgg ttctgaaggt
     6541 gcaccctgtg ggaacactgc gtcttccgct ccgcattcgt gcccatgtct ctcatgtgct
     6601 gctggcggta gctgcggggc tgcgccatgg catcatttct cttgaagctc ctctccgtga
     6661 cgcctgtaaa aaagcagcga gattcaaaga agcccttttt cagggcgtgc atgtgctgga
     6721 tgacagttac aacgccaacc cggcaagcat gaaagcatct ttggaagcgc tccgggacta
     6781 tcccgggcag cgccgcatag cagtgctggg agacatgttt gaactgggaa aatcagcgga
     6841 agccctgcat gcggatatcg gtcgttttgc ggcgtccctg tctattgact tccttttatc
     6901 gcttggcggg tatggcggta tcatggcgga ggcggcacat cgggcagggc tggatcaggc
     6961 atttgcctgt gccagccacc aggaggctgc agcctggctg cagaataaca cggcggcggg
     7021 agatgcggtt ctgctcaaag gctcccgggg catgaaaatg gaaacagtat ttgagcagtg
     7081 gaaaagtatt gccccggccg ggcaataacg ggtggatttc atggcatatt ggctgtctgt
     7141 tcttctgaaa gatgaggtgg gcttcaccaa cgtattaagc taccacagcg tccgtgcggg
     7201 cggtgccgct ttcaccgctt tttttctttc catcctttta ggtccggcaa ttatccggag
     7261 gctgcgccag ctgaaaatcg gacagtatat cagagaagag catgtcgaaa gcctgcacga
     7321 acttcataaa ggaaaagcag gaacacccac catgggtggc ctgatgatca tcgtttccac
     7381 ccttgccgct ctgcttctgt ggggcagact gtcgaaccgc cttctatggg tttccatgat
     7441 tatcctcctg gtcatgggcg ctttggggtt tatggatgat ttcatcaaac tgaaacgcaa
     7501 acataatgca ggcttgagcg cccgggcaaa attcgccggt cagattctga cgggtctgct
     7561 tctgggtatt tatctggtca ataaccccat tactgtcagc gaatcctatg tgctgcatcg
     7621 ggacgtcatc aactggcctc ttctggaatc gatgctggca ggcgcacacg agcgaagcca
     7681 gaccccggat gtaaaaaaaa tctgcagtat gttgtccccg gagtgcagga gcatcatcag
     7741 aggaaatgta aatgaagcac agatcaccga tgaagaacag cagacagtgc tgaaagaact
     7801 caatctggca cttcgcagca cagagctcta cgaggaagca ctctggcacg acattgtaaa
     7861 gaacccggaa gcaagacggc tcctccagtc ttcccctgag aagatgagtg aacgggatct
     7921 gatccgcttt aaccggctgt tactggagca gagtttctcc ggaatgattg cggaaagcgt
     7981 tccgaacctt cataccaagc tggggattcc gggattcaaa gaactattta tccccctggg
     8041 cttcttttac atcttcttcg tcacacttgt catggtcagc ataaccaacg ccgtcaatct
     8101 taccgacggg ctggacgggc ttgccgccgg cgtttccatc atttccattc ttgcctatgc
     8161 agccatcgcc tatatcatca gccgggcaga ctggtcacgc tatcttttcc taacctatgt
     8221 tcccgaggca agcgaactct tcgtcttcgg ggcggcactt cttggctccg gcctgggttt
     8281 tctctggttt aacggacatc ccgcagaagt cttcatgggc gacaccggct ctctggcatt
     8341 gggcggagcc atcggtgcgc tggcgctgct gaccaaacag gaactgctgc ttcccgttgt
     8401 tgccggtctt tttgttctgg aagcagcaag cgtcgtcatt caggtatttt catttaaact
     8461 gacagggaaa cgtgttttcc gcatgtcgcc gctgcatcat cattttgaat tgtgcggctg
     8521 gaaggaaacc aaagtaaccc tgcgtttctg gattcttgcg tttctgtttg cgcttctcag
     8581 tctgggcgca ttaaagcttc gttgacaaac accgggaaaa tatggatctg acaggtaaac
     8641 atatccttct agtggggctg ggccgctctt cggtggcagc ggcgcagctc attatggaaa
     8701 agggagggat gccttttatc agcgagcgga acgacggaca atcgctggaa aactggctgg
     8761 agatctgtcg cacccgggga attcctgtgg aaacggccgg gcatacatct cccctctttc
     8821 aaaccgccga tctcgttgta atcaatccgg gtgtccccta taacgccccc tgcctggaca
     8881 taccgaaaga aaaaggcata cccgtcgtcg gagaattgga attcgcttcc tggttcaatc
     8941 actctaccgt tattgccgtc accggaacca atggaaaaac caccgctacc agtctccttt
     9001 acaccatgat ccgcacctgt gggtatagtg tcgctttggc aggcaacaca cataccgcat
     9061 ggtcggaagt tactgcaaaa gaagaacaac ctgaatatac cgtgctggaa gtaagcagtt
     9121 atcagatgga aacggcagat acatttcacc cgaaagttgc cgtcattctc aatcttaccc
     9181 cggatcatct gggcaggcat ggaaccatgg aatgctatgg cgcaacaaaa gcacgtatat
     9241 ttcaacgcat gcagtcaggt gacacagtca ttctaaataa agattacgcc gctttacgcg
     9301 ccttgcctct tccttttcag gcagaaacag tctggttcgg aaaagcaagg gatggcgata
     9361 aaacgggttg gagctatgat accgcccact tctatcagga cggcgaaatt gccgccgatg
     9421 caaaagacag tccactgccg ggactgcata ataaagaaaa tattactgcc gtgcttgctg
     9481 ccgctcatgc actggggctg gactggggcg gagttttaca gggactgcgg cagtttgcag
     9541 gtgttgagca ccgcattgaa tatgtcactg ccatcaacgg aatcgcattt ttcaacgatt
     9601 caaaagccac caatatagac agcctccggg tggcgctgga aagctttgtg cagccggtgg
     9661 ttctgattgc gggaggagaa ggaaaaggca gcagttatga accgctccgt tctcttatcc
     9721 gtgaccatgt cagtaccctg ataactattg gcgaggaagc gccggctctt gaagcatgct
     9781 ttcaaaaaga cgttttcatt tgccggggaa caaccatgaa agacgccgtt tacaaggcat
     9841 atacggcggc ttcacccggc agtgttgtcc tcctctcccc tgcctgcgcc agttttgact
     9901 ggtacagcaa ttatgaggag cggggcggtg atttcaaaaa ctgcgttcat gcgcttaaag
     9961 aagatctgca tacaggcgag gagaaaggaa aatgaaaaaa gaaaccaata cactgatcat
    10021 ggctgtactg gcgctggttc tgctgggcac actgatgatg tacagtattg atgccgtgcg
    10081 cgggcccaaa tccatgattc tctataaaca tctggcgttt ctcggcttag ggctgatcgg
    10141 ttttttcttc acaatgatac ttgattacca cgtgctcgga agtccggcgg ttcggaaagc
    10201 atttctgatt ggcacgctga ttctccttgc gtgcactttt attcccggtc ttcggctgag
    10261 catgggcggc gcttatcggt ggatcggatg gaaacagctg agttttcagc ctgctgaact
    10321 ggcacgtttc acccttgtac tcatgctcgc tgtccgatta tctaaacagc aggataatat
    10381 gagctattgg acaaccagcc ttgtaaagcc ggggttttat ctcgggctct tcgccgggat
    10441 agttgtgctg caaaaggatc tcggaattcc tgtcgtcatg tccgttgcca cgttcatcat
    10501 tatatggact gccggcgccc gcattgccca cctgggtctt gcaggtgcgg taggtatcac
    10561 cgcaattatc ccgcttattg ccatggaagg gtacagagcg acccgaatta aatcatttat
    10621 ggatccctgg gcagcacggg atagtcacgg ctatcaatta attcaatcgc tctcttcttt
    10681 ctatcaggga ggcgtctggg ggcgcggtgt aggtttcgga gagcagaaac tcggttatct
    10741 cccggctgca catacagatt ttatctttgc catgatcggt gaagaactgg ggctggtggg
    10801 aaccattgtg gtagtgctgc tatttacagc cattctcatt gcgggcattc ggactgtggt
    10861 gcgggcgcct gatctgttcg ggtctcttct ggctgtcgga attacgacgc tcattgtctt
    10921 tcaggccttc ttcgtcatgt tggttaacct tgggtggctg ccgaccaaag ggcttcctct
    10981 ccccttcgta agctatggcg gcagcgccct gatgaccaat ctgtggatgg caggcgtgct
    11041 gatcaacatt gcttctcagg caagtgaaaa ggaaaaaacg gcgagaagca gtgtttttac
    11101 tcattcacga ctgaaatacc tgttccgcat tttccttcct aaaaaaccgg cgacagtacg
    11161 tatctgactt tcggtgtaag gcgatacaga caggctggaa tcagacatgc gactcagcaa
    11221 agcatgctgc gtccctcatt cggttcagcg cctccacgat cgtatccgcc tcttcaaaac
    11281 cataggaaag gcgcagctgc cgcattccgg catccttgag gcttcccccg ggatgtacac
    11341 agaattctcc cgggatataa acgacccgcg gataaggatc tccggaagga ccgtcatatt
    11401 tttcaatgcc tgtggttcgg gcaagatagc ggaaaaaagg ggacgtactg tcggtgcgga
    11461 tgttttaaaa gtcagataat agtagaaacc ggctgtaccg ccgcaaactt cttccagcca
    11521 gggaccgagc atttcccgga tggcctgtcc gacaatctcg ctttttgctc tgtagcctgc
    11581 cttcacacgg gccacttgat cgtgcatata acgacacagc atcaccgctg ctatttcctg
    11641 attgagcagt ggcgcgctga atcccacatc gctggttttt tgtacaagcg cctgcatcag
    11701 aacgccggga gcgccaagca taaacccgat gcgcatggca ggagccagca ctttcgataa
    11761 agtgccgatt tcatacacag gtgcatccgg agtatcgacc aacgctgagc ggggcggctc
    11821 cgcctccggg tcgtggatca gccattcata ggcctgatca aaaaagaccg gacagagacg
    11881 acccagccgc gctgaccact cttttgccag ctgtgcaagc ccccggcgat aggcattgca
    11941 caacacgttc ccgctcggat tattcacagt caccacgtag aaaaaagaaa gcctttgtgc
    12001 ctgatcaccc agcgattcca gttttcgccg gactgatgcc gggttaagcc cctctttctc
    12061 ttccggcact gtaatcacct gaaacccctt gcgctccaga tgattgcagt agatgtaata
    12121 catgggatca gcagtgatga caatgcccgg ctcaaaaata tctgccagcg cttccagaat
    12181 gctcgtggca ccgctcgggc cgatgaccac ctcctgtgct gacatcactt cttcagtcat
    12241 cccgccaatg gcttcctcca cgtaataatc acgcaacgcc tgaatcagac cgggagcacc
    12301 ctgtggcccg ccgtaattaa aggcctgtcg gaagtcctgc gggcaggaag caatctcttt
    12361 caccacctca gccagcagct ctgtcggaat ggtcttttca ttcacataac ccaccccgag
    12421 gttgatgtct tttgcatccc gaaaatctgt ggcaaaagct gccatcatgc ggttcaccgg
    12481 cgacggctcc accgatcctc tgccataact ggaaaaagaa tagttcataa tcatgctgct
    12541 ttgtttattg aacgataaat aaagaaaacg aaccttccgc tttctcccta aagattatgg
    12601 caaagaatct ccaccgacgc aagagagaaa caaccatctg ggcgtcttga attaccggcc
    12661 tgcatcgcct gcgcccacaa aaaaaatccc tacccgttct gcttggcaga gcgggcaggg
    12721 atgaatgatt aaattcaggg ggaagctcag gcctggacgt tgcggcggcg ggctttcacg
    12781 cgggcagcct tgccggagcg gtcacggagg tagtagagtt tggcacggcg gacacgacct
    12841 tcgcgggtca cttccacttt ttcaatgcgg ggcgaatgca gcgggaacac acgctccaca
    12901 ccctcgttga aggcaacacg ccggacggta aaggtagcgg tggggctatc tttgccttta
    12961 tggctgataa caacgccttc atagacctgg acacgctctt tttcgccttc gacaatgcga
    13021 aaatgcacgc gtacattgtc cccaactttg aatttgggga gtgcctggct ttctttgcaa
    13081 tactggcttg cgtattcctc gactaaattc acggtaagtt ctccttattg gacggcggca
    13141 gatgcacgcc aatctcggca agtaattcca agtcttcttt gcttaagtta gtcaataatt
    13201 cagggcgtcg gatccaggtt gcccggagcg cttccctgcg tctgtatttt ctgatagcct
    13261 catggttgcc ttcgagcagc acccgaggta cttcttttcc tctgaacacg ggcgggcgcg
    13321 tgtactgggg atatcccaga atgccatggt aaaaagaatc ggtatccaca gattcccagt
    13381 tacccactac gccgggagcc atgcgactga cggcttcgac caacaccagt gcggccgctt
    13441 cgcctccgct cagcacataa tcaccaatgg agatttcttc ggtcaccagt gtttcagcaa
    13501 tgcgttcatc catgccttca tatcgggcgc agaggagcac catgtctttc caccctgcca
    13561 tttcccgaac cagattctga tccagtttcc ggcctcgggg tgaaagaagc accacatgtt
    13621 ccagagagtt tttactgatt aaactttcga ctgcctcaaa aacgggcgga caggtcatca
    13681 ccatgcctgc tccgccgcca taaggcgcat catccacagt ccgatgccgg tcatgggcga
    13741 aatctctcgg attcaccaga ttgaccgtca gtatatcttt ggcaatcgcc ctgcccaaaa
    13801 tgccgatacg aagtgccggc tcaagcattt ccggaaagag cgtgaggatg tctatccgca
    13861 tgacactgtc cagttctgta cccgaaggct attcgtttcc cagggcatcg tccatcacca
    13921 gaaaaggctc cggatcgaca acagcgattc tttttttatt tatatccaca gaggcaatga
    13981 cttccgcaat cacgggcagt gcagcggtgc gcccctcacc ggtgaggaga cgaattaccc
    14041 cgcctgccgg agtatcgata gtttcgaaaa ccacgccaag aagacgaccg ccgggagcaa
    14101 tgactttaaa gccttcgagc tcttccagat cgggcatgtc tgccgagact tttttgcagt
    14161 ctctgctgtc caccgcaact tccgcccggt gcaaggcctg catttcatca cggcagatac
    14221 cggcggttaa agccacacgg gcagatgtgc cgttgcgttc gcaagtttct attttcgccc
    14281 gaagcgggac tccgtcgacc gtggtaagcc agatgcggtc gcagtcatcc agcgactgaa
    14341 gatacttatg tttcaactgt atgcgcaggc tgcgtctggc agcctccaca gcaagtacct
    14401 gaccgatcac aatccgttca ggctgattca ctgcgcttct gtctggttgg taatttccaa
    14461 cccgagattt ttttcgcttt tcgtaccggc agcgtaaagg agtgtacgca gggctttaat
    14521 ggttcgtcca ttctttccga tgatacgacc catatccgct tcgtccactg tcagctgcac
    14581 ggtttcgccg gtatcgctct caataagccg cacgctgact gaatcaggac aggtaaccag
    14641 ttttttggct acaaattcga caagatcttt tgctgacatt atgcctcttc cgcaggggtc
    14701 tcttcagcca ccgcttcaac ggattcttcg acggctgctt cttctgcttt ttcttctacg
    14761 ggcgctttgg cagccttggg cggcggcggg gcattgtaac ctttgtccac aacagcctca
    14821 cccttccggg ttgcgacagc ctcttcgggc cgtgttccgt catggaagtg cttcagcaca
    14881 ccgagtttgc tcatgacaga acgggcggta tcggacattt gagccccatg tcccagccag
    14941 tcgagcgcac ggtgtatatt gatttcgctt atcggctccg ggcgtgccgt cgggtgataa
    15001 aacccgagaa tgtccagttc ccgcccgcgg gtgcgtgtac gcgagtcctg aaccacaata
    15061 cggtagtagg gctgtccctt ggaaccgccg cgtttcatac gaatcactgt cgccatgatg
    15121 atcctctctg tagcctggct tatgctacgc attctttaaa cgtacgtcag aaaactaaaa
    15181 acaagtacct gtatatatga acctgccttg aaggtcagac aggctgttgc actgaattga
    15241 ttcagagccc tggcatggga ggcatgggac cgccgggcat tctgcccatt ttccggcctt
    15301 tgccgccttt catcgcctgt ttcatcatca ctttcatttt ttgaaattct tttaaaaggg
    15361 cattcacatc ctgcacggaa agaccgctcc cgtcggcaat gcgcctgcgt ctgcttccat
    15421 tgatgatatt gggattgcgc cgctctttga gcgtcatgga cagaataatg gcttctgtat
    15481 gcttcatctc gtcttcagcg aaatccatcc cctggggcat catcttactg atgccgggga
    15541 ttttctgcag cagttcggtc atgctgccca tcttcttcaa ctgtcgcata tgatccagaa
    15601 aatcttccag atcccaatcg cctttgcgca cttttttctg gaagtgcagc gccttttcct
    15661 ggtcgacgac ctgctgcgcc ttttccacca gagaaacgat atcgcccatt cccagaatct
    15721 ggtcagccat gcgctgggga tggaagggct ccagggcttc cagtttttca ccggtaccca
    15781 caaatttaat ggggcagcct gtgacatcga tgagactgat ggcagcaccg cctctggcgt
    15841 caccgtccat ctgcgtcaga ataacccct
//
LOCUS       DAOPWB010000076        15866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_3766_length_15866_cov_4.856745, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000076 DAOPWB010000000
VERSION     DAOPWB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 15866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15866
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3766_length_15866_cov_4.856745"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKN92_08280"
     CDS             complement(<1..308)
                     /locus_tag="PKN92_08280"
                     /inference="COORDINATES: protein motif:HMM:NF021988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNZ48829.1"
                     /translation="MIKESMSDAGSLYANEWLSRLTESRHDVFLQNIYGSDQQVLAER
                     KKLLHQAILAFLVRFGDLPVRIFRAPGRLNLRGMHVDTHGGWMNLMTHQREVVMVAAA
                     S"
     gene            598..1974
                     /locus_tag="PKN92_08285"
     CDS             598..1974
                     /locus_tag="PKN92_08285"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ48830.1"
                     /translation="MKPGNRKGRVVAAGLLLAMILLTGCGGGKGKHDLLVSVTIDTLP
                     VQGASVLMMGVDQGVTPVTVHEVPPGDYEVILRLDKYRRAIESIKVTEEPEQIFRIEL
                     EPILGFLSITTQPADAEVLLNGESLGKAPIVKHALQVGEYSYEVVHPDYYPVQNTFVM
                     EDNFKMEFTHELRPLESELIVTSRPSSASIWLNNLPQAQKTPAKLVLRPGRYLVSTYT
                     DGYVQVDELVDLKANESHSVEMRMSPGKVPQGMTLVPGGEFTMGADDSAPDERPARKI
                     MVKPFYMDRFEVTNQAFKQVFPDHKYLEGQDSLPALGISWTQAVKYCETVGKRLPTEA
                     EWEKAARGTDGRIYPWGEVFVPGYANTSESGIGVATRVGNFFEASSVYGCMDLSGNAY
                     EWVFDWYEAYPGNTDVTKDYGQIFRVLRGGSYKGDKFEARSAARHFDRVDSNRRDYGC
                     RCAMDARD"
     gene            2089..2385
                     /locus_tag="PKN92_08290"
     CDS             2089..2385
                     /locus_tag="PKN92_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNZ48831.1"
                     /translation="MKTNPLPHEKILYICLNERETGECCGGKGSAALHGKLKSAIRER
                     GLSSRIRISRSGCQGRCGDGPNAQLFPDNLWFSGITESDIDLLLDKLTEDSPSR"
     gene            2417..3196
                     /locus_tag="PKN92_08295"
     CDS             2417..3196
                     /locus_tag="PKN92_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNZ48832.1"
                     /translation="MATQETRPVQTRSSNGILQIIMNRPEKKNALTQEMYALMTDALT
                     YAAEEGSIRVAVFQGTDGVFCAGNDLQDFLGTPISEKSPVFQFIESLIAFPKILAANV
                     EGVAIGIGTTMLLHCDLVYASDNAFFQLPFSRLGLCAEAGSSVLLPRAIGMTRAATLL
                     TTGDSIDSRTAAEWGLISAFFPEEIFEAEAMKRLGQVAAMPPAALRHTRALLRKPLEE
                     EVRAAVLRESRQFQECLQGAEAAEALRAFLEKRPPDFSSFA"
     gene            complement(3205..3507)
                     /locus_tag="PKN92_08300"
     CDS             complement(3205..3507)
                     /locus_tag="PKN92_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HNZ48833.1"
                     /translation="MQEKLYAEFKSASLILRDHLAIDRTLLANERTLLAYLRSGVAML
                     MAGFTILHFLDGGWFWLVGLFCLPVGILVGGIGVWRFVRMNRAISLVRNRSAVDDR"
     gene            complement(3550..4425)
                     /gene="lipA"
                     /locus_tag="PKN92_08305"
     CDS             complement(3550..4425)
                     /gene="lipA"
                     /locus_tag="PKN92_08305"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNZ48834.1"
                     /translation="MQKNTSSQKFPSWIRTAWPQRGAVDEVRDTLGALSLNTVCRSAH
                     CPNQGECWSRRTATFMILGAVCSRNCAFCSVDGGVPLPPDPEEPARLAEAVNRLGLRY
                     TVITSVTRDDLPDGGAAHFAAVIRALRSQCPGVAIELLTPDFGGDDGAIKTVTDAAPE
                     VFGHNVETVPRLHSLLRDPVASYERSLSVLKRARDWLPESSFVKSGLMVGCGETAEEV
                     LAVLQDLRAAGCDAVTVGQYLKPGKEHLEVQEFIAPEQFDLYAQEARRLGFRFVMSGP
                     LVRSSYRAETILSAE"
     gene            complement(4623..5888)
                     /locus_tag="PKN92_08310"
     CDS             complement(4623..5888)
                     /locus_tag="PKN92_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNZ48835.1"
                     /translation="MRSLYSLMIVLAALVLITGCGAPKPAPAPQSTAQKAVAPESAVS
                     GSSSEKEVPQIEIVKEAPDSEGAAATETAEDAAPAPAGSAPKLVVPEPEYDFGKMDNS
                     EMVEHGFLIRNEGGSLLKIESVRASCGCTTTALKKKELEPGEELIIQANTNLKGRQGP
                     QTKAITIFSNDPENPNYRLTIKGEAIASISLDPMNVSFGRIEDDEPREATVTIKSNKE
                     DVNFTVRSAEVDGMTFIQQEVKEVVPGKEYQMVVKTQPQLPEGHHNGRIIIRTDSTER
                     PVIWLTVSMQVIGALQIMPPLVNLRYTEEEGAVETQQISLTKGRISEFEITDVILPLD
                     SMEHELIKAAPNVYRLVLKNMPRNDALEGKRVVVKTNREDYPEVYIPFNIYRPKLQSI
                     PPQKVTDAAKSLNAAIVNKEIEEAIEANE"
     gene            complement(6026..9010)
                     /locus_tag="PKN92_08315"
     CDS             complement(6026..9010)
                     /locus_tag="PKN92_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNZ48836.1"
                     /translation="MAIGKKKKKNISSSFPTEAAPAERFIQIGGGGEAEADMGLRSLW
                     PDHFAPPSRALFLASSWLILTVLLCAAASFGAFTAVKESLAALLGLQFPDQVDPGNFD
                     MAFVISALSGTVLFSLIHPSLGIGLIILARTWLDGYTFPLDNLYFVWAIYLLALIWLV
                     RVFHGKDRPLTFPPPLVVFSLLIVYLFLTLPFSWQYGQSYPLVWLWVGYLTLFAVSLH
                     VTGVPRIRKLLLFLFLAAMGAQALFSVLHFSFLLPYLRTIVQNPAILQRYFNTDVITP
                     ELARRFMVNRAFGSMLFPNALAGYLILGIPFAFALCWATAGSYKQALRELRRKARTVT
                     ELRERALLLGISVIPGMLVFILFLFVTYFPKEYWLEETPLPWYYSLSVLILFAMILAC
                     ATALFCFSLLCRCSSLSSWWALLRFPALLALFPLLLYTLWITYSRGAFLGLLASSGFS
                     FLLYKINPGSGLPRFFRRSAALLMTLTLVLLISLILVQSFCAPPSWAQQPGSAGESKP
                     SVAITSEGVAVTTSDLADPTTFGFRLGYWQVAARLFFANWQWGTGLGNFQIGYPVYQF
                     MGAGDVREAHNGYLQFFCETGLGGGLLFLAFWAYFVIWGAASVLRQQDKKEKRILAGM
                     YAGILAFALHAALDINFSHPSLMMLALFFCGAFYGRVSSGSPMHGATAEGEVPGERAL
                     PSLPFMQRFMSVVLALGLLLAGNASFRLYKQNIALNRLRLVNLSWDEQQKMRFQNLQF
                     FLLEVPSYALARDRGEDIKKPPRLPLSAARMLIEDETVLTTAFAFYRPDPQVPGKFHR
                     IQEGEIVPESALAIVRKPWYLFKRAIPAGLKRIDELIAQDRRFPHSTELALHIASWYE
                     IYAQRVFNDAVADQRPGWEANFLEWTEEVLRRNPYHADLYRFKGNAYLWLVLGREKDE
                     ESDRSYLEKAKASFEEMLKYCPLSSGHRYVYAGAMEKMAEWYRAKGDAEEAQRCLDKM
                     GALRAEAFGLDTQRAHSHVYPR"
     gene            complement(9015..10115)
                     /locus_tag="PKN92_08320"
     CDS             complement(9015..10115)
                     /locus_tag="PKN92_08320"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNZ48837.1"
                     /translation="MIPRQYAVFTYVLILSFALSLALTWVMQKLARRWNILDHPAGRK
                     SHDKSTPLLGGAAIFITFYIVLIGHVVAVSWFWPWGPAWLAQKLAMLLGEDHGQKMVG
                     ILLAGAMIFILGIVDDLHVLRPWFKLLGQIAAALVLVFFGMRIHLFYLHDVFSSSFIT
                     VLWVVLIVNSMNLLDNMDGLSGGVSIIAAITFFLSVQPYDTGFMVRFMLVIFAGAVGG
                     FLFFNLPPARIFMGDAGAMFNGYLLATVAVVGTFHIEGVNSRAAVVSPVLALSVPLFD
                     TLSVVWIRWRNGDSIMLGDKRHFSHRLVEVGMRPAMAVYFIYMVAALVGLNGALLPML
                     SLMGTGIILAQTLGIFLLIVLLMNAGKRNGSE"
     gene            complement(10243..11361)
                     /locus_tag="PKN92_08325"
     CDS             complement(10243..11361)
                     /locus_tag="PKN92_08325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ48838.1"
                     /translation="MRVLHVYKDYDPPVRGGMERHVALMCRYQRQWCDVEALVCSREM
                     RTRRFIHEDVPVTAVGEWCRFQSAPLAPLFPWYLGTTRADVVVVHFPNPTAEMSWLLA
                     RPKAALIVRYQSDVIRQARAMRVYRPFQHAFLRRADLILPTSHQYLDSSETLQHFREK
                     CEVLPLGIVPEAYQSPDPEAVSALREKYGSFVFFCGVHRYYKGLRWLIEAASAIQGRV
                     VIAGDGPERKANEALAGSCGGEVLFPGALSEEDLVNYLHAAAVVAFPSSERSEAFGLS
                     ILEAHACGTPVVSTQLGTGVEFVNEDGMTGLNVPPKNAPALAAAVNRLLQDRELADRL
                     GQAARDRVFRAFSAEKIARQEYEIYQRVVEARRRSSGL"
     gene            complement(11416..13284)
                     /locus_tag="PKN92_08330"
     CDS             complement(11416..13284)
                     /locus_tag="PKN92_08330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ48839.1"
                     /translation="MEKKLSYNPDWQQEYADITVSAAEAVASIEPGDRVFLGTGCATP
                     TVLVEALVGRARDLTDIEIVQLLTRGDAPYATKELSSSFPVNSFFISQNVREIIQQGM
                     GDYTPIFLSDIPRLFTTGRLPLDVAMIHVSPPDEHGMCSLGVSVDIVKSAAENARIVI
                     AQVNPQMPRTHGNSTIHVYDIDYLVPVDLPVLENIQPEPRPGTEDIGEYLAALIEDGS
                     TIQLGIGRVPQAVAPFLKKKRDLGMHTEMLTDSIIELVEEGVINGSQKSMDRGKIVAS
                     FVMGTKRLYDYIDNNPVFSMNPTEYVNDPFIISRQNKMVAINVALEVDLTGQVCADSI
                     GTQFYSGIGGQVDFNRGAAGAEDGKAIIALYSTTTDGKSRIVGTLAPGAGVVTTRGDV
                     HYVVTEYGVAYLHGKNIQERALALISIAHPDFREQLLKEAIANKFVRAELSEYTGKIT
                     VGPKETRTVLRLADGQQLTVRPIHPTDEGKVRELFHDLSQKSLYTRFMARMKWVPRKQ
                     VQQFVYIDHRNEVSIVATIPEADGEAIIAIGGYYLDLKTNRAEVAFVVHDDYQQKHIG
                     SFLFKQLCTIAKRNGIAGFTAEVLDENKSMQAVFNNSGLTIQTELREGVYHYDMTL"
     gene            complement(13292..15385)
                     /locus_tag="PKN92_08335"
     CDS             complement(13292..15385)
                     /locus_tag="PKN92_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNZ48840.1"
                     /translation="MLDGLLYPNSVAVIGASRTPGKVGSAILSNLINGGYQGKIIPVN
                     PTTDELFGLPCYKDVRDAGINIDHCVIVVPQRFVMDALAGASVVRAKAVTVITAGFKE
                     VGAEGAAKEKEMVAFCRKHSIRILGPNCLGLINTENRMNASFAAQMPKPGNISVLSQS
                     GALCTAILDWAEGRGVGLAKLLSIGNKADLCETDLLRALAQDKQTKVIAAYLESITSG
                     PEFIRVAEEVSQEKPVVILKAGTTAAGGKAAASHTGSLAGADIAYGAAFTRAGIVRAD
                     NFEALFDYATALAMQPLPKGNRVAVITNAGGPGIMAADAVETLGMRMDALTPEIAATV
                     KAALPPAASVANPIDVLGDAESDRYAMAVKEVLADPQVDSAVVILTPQAMSDCEATAQ
                     AIIDAEHHGKPVLTCFMGGSQVNEARNMFVAAGVPDYPSPERAVAALKSMVDYASWLG
                     RPSRIVTRFPVNRHRVERIFARQERSGRKEMGEVEAKEILKAYDFNVPAGALARTGEE
                     AVAIAQRVGFPVVMKIVSPDLLHKSDFGGVKLNLHTPAEVQDAYDLMMLRIRRRAPEA
                     HLRGVLVEKMGKGGREVILGMHRDPQFGPMLMFGLGGIFVEVMKDVSFYLAPLTHEEA
                     LEMLKNTRSFALLRGVRGQAGVDLNAIAAALQRISQLVTDFPQIEEMDINPFMVGEVG
                     TAPIVADARMILSGE"
     gene            complement(15785..15859)
                     /locus_tag="PKN92_08340"
     tRNA            complement(15785..15859)
                     /locus_tag="PKN92_08340"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15825..15827),aa:Cys,seq:gca)
BASE COUNT         3965 a         4399 c         4182 g         3320 t
ORIGIN      
        1 gacgctgccg ccaccatcac cacttcccgc tggtgcgtca tgagattcat ccatccgcca
       61 tgggtgtcta catgcatgcc ccggaggttc agccgcccgg gtgcccggaa aattcggaca
      121 gggagatcac caaagcgcac caaaaatgcc agtatagcct gatgcagcag tttttttctt
      181 tcagcaagaa cctgctgatc tgatccgtag atattctgaa gaaagacatc gtgacggctt
      241 tccgtcagtc gactcagcca ttcgttggca tataaactgc cagcgtcgga catagactct
      301 ttaatcatta tttgtaaccc aggacaaaga atgcttgccg catgaaaaat aagggagaaa
      361 acggtctgat cacttattat atctaaaaat caataaagaa ggcatacttt taacctgatc
      421 agcgtttact ttaacaaaaa gactgccggc atgaatttcc ggtttcgact catatgcata
      481 tagtcagcag tttaatggta tacttgtata tatttatatt gctgatactg tgtctgtggt
      541 gtggtcttat atttttttat tttactggtc ttatataaat tgaaggaaat ggcagacatg
      601 aaacccggaa accggaaggg acgggtcgtt gccgccggtc ttttgctggc aatgatcctg
      661 ctcacggggt gtggcggcgg gaaggggaaa cacgatcttc tggtcagtgt gactatcgat
      721 acactgccgg tgcagggcgc ttccgtcctg atgatgggtg ttgatcaggg ggttacgccg
      781 gtcacggtgc atgaagtacc gcccggcgac tacgaggtaa tccttcgcct ggacaagtac
      841 cgccgcgcca tagagtcgat taaagtaaca gaggagcccg aacagatttt caggatagaa
      901 ctggaaccga ttctgggctt tctgtccatt accacccagc ctgccgatgc ggaggtgctg
      961 ctgaacggcg agtctctggg aaaagcacct attgtcaaac atgcgcttca ggtaggcgaa
     1021 tacagttatg aagtagttca cccggactat tatcccgttc agaacacctt cgtcatggaa
     1081 gacaacttca aaatggagtt tacccatgag ttgcggcctc tggaatcgga actgatcgtg
     1141 acttcccggc cgagttccgc cagtatctgg ctgaacaatc ttcctcaggc acagaagaca
     1201 cccgcaaagc tggtactgcg cccgggtcgt tatctggtca gcacctacac cgacggctat
     1261 gtgcaggtgg atgagctggt cgatctgaaa gcgaacgaat ctcattcggt ggaaatgcgc
     1321 atgtcgccgg gcaaggtacc gcagggcatg acactggttc ccggcggtga gtttaccatg
     1381 ggtgccgacg acagcgcacc agacgagcgc cccgcccgta agattatggt aaagcctttt
     1441 tatatggatc gctttgaagt aaccaatcag gccttcaaac aggtttttcc ggaccacaag
     1501 tatctggaag gtcaggacag cctgcccgct ctcggcattt cctggactca ggcagtgaag
     1561 tactgcgaaa ccgtaggcaa gcgcctgcca acggaagcgg aatgggaaaa ggcagcccgc
     1621 ggtacggacg gacgcatcta tccctggggc gaagtgtttg tgcccggcta tgccaatacg
     1681 tcggaatccg gcatcggtgt ggcgacacgg gtcggcaatt tttttgaagc ctcctctgtt
     1741 tacggatgta tggatctgtc gggcaacgct tatgaatggg tctttgactg gtacgaagcc
     1801 taccctggca atacggacgt caccaaggac tacggtcaga ttttccgtgt cctccggggc
     1861 ggctcctaca agggcgataa atttgaggca cggtcggcgg cgcgccattt tgaccgggta
     1921 gattcgaaca ggcgtgatta tggctgccgc tgtgccatgg atgcccggga ctgatccttt
     1981 acgcagaaac tgacaaaggc actgatgccc gtcccgctgt agcactttcg cacaacggtg
     2041 tgcgtgcggc tcttttttta acagtaacgg agtcctgaaa tacaggccat gaaaacaaat
     2101 cccctccccc atgagaaaat tttgtacata tgtcttaatg agcgagaaac gggtgagtgc
     2161 tgcggcggaa aaggctcggc cgcactgcat gggaaactga aatcagcgat tcgggagcgg
     2221 ggcctttcct cacgcatccg tatcagccgt tcgggatgcc agggcaggtg cggagatgga
     2281 cccaacgcac agctcttccc ggataacctc tggttttccg gcattaccga atcggatata
     2341 gatctgcttc tggataaact gacagaagac agcccttccc gttgacacgt ctgatgtaaa
     2401 caaaaaggaa gaccccatgg cgacacagga aacccgtccc gtgcagaccc gcagcagcaa
     2461 cggcattctg caaattatta tgaaccggcc ggaaaagaag aatgccctga cacaggagat
     2521 gtacgctctt atgacggatg cccttacgta cgccgcagaa gaagggagta tccgtgtcgc
     2581 cgtgtttcag ggcacagatg gcgtgttctg tgcgggcaat gatctgcagg attttctggg
     2641 cacgcccatc agtgaaaaaa gccctgtatt tcagtttatc gaaagcctga tcgcctttcc
     2701 caaaattctg gcagccaacg tggaaggtgt cgctatcggc atcggcacca ccatgctgct
     2761 tcactgcgac cttgtctacg catcggacaa cgcttttttt cagcttcctt tcagtcggct
     2821 gggcctctgt gccgaagcag gatcaagcgt actcctcccc agagcgatcg gcatgacacg
     2881 ggcggcaacg ctgcttacta caggcgactc cattgacagc aggacggcag cggagtgggg
     2941 actcatcagt gcctttttcc cggaggagat ttttgaagcc gaagccatga aacggttagg
     3001 acaggtggca gccatgccgc ctgccgcgct gcgtcatacc cgtgccctgc tcagaaagcc
     3061 gctggaagag gaggtgcgtg cagctgttct ccgggagtca agacagtttc aggaatgcct
     3121 tcagggcgca gaagcagccg aagcactgcg cgcctttctg gaaaaacgcc ccccggactt
     3181 ctcctctttc gcttgagggg gagatcatcg atcatcgacc gccgaccggt tacgtaccag
     3241 agagatggca cggttcatcc ggacaaagcg ccacacacca atgccgccga ccagaatgcc
     3301 gacaggcaga caaaaaagcc cgaccagcca aaaccagcct ccgtccaaaa aatgcaggat
     3361 ggtgaaaccc gccataagca tggcaacacc cgaacgcagg taggcaagca gtgtccgctc
     3421 attagcgagc agagtccggt ctatggcaag atgatctctg agaatgaggg aggctgattt
     3481 aaattcggcg tacagttttt cttgcatgac gtgttcttcc gtggctgaag gaaaattacg
     3541 gggcacctgt cattctgcgg agagaatcgt ttccgcacga taggaactgc gcaccagagg
     3601 cccggacatg acaaagcgaa agcccagacg acgggcttcc tgcgcgtaca agtcaaactg
     3661 ctccggcgca ataaattcct gcacttccag gtgctctttg cccggtttaa gatattgacc
     3721 taccgttacc gcatcacatc ctgccgcacg cagatcctga agaacggcaa gcacttcctc
     3781 tgccgtttct ccgcagccga ccataagccc tgacttgaca aaactgctct caggaagcca
     3841 gtcacgggcg cgcttcagga cagaaaggct tcgttcataa gatgccacag gatcccgcag
     3901 caggctgtgc agacggggca ctgtctccac gttatgcccg aaaacttccg gagcagcatc
     3961 ggttacagtt ttaatcgccc cgtcatctcc gccgaaatcc ggcgtgagca gctcaatggc
     4021 aacgccggga cactgcgagc gcagtgcccg gatcactgcc gcaaagtgag cggcaccgcc
     4081 atcgggcaga tcatcacggg tcaccgatgt aatcaccgta taccgtagtc ccagccggtt
     4141 cacagcctcg gccaggcgcg ccggctcttc gggatctggc ggcagaggca caccgccatc
     4201 cacactgcaa aaggcacagt tccggctgca tacggcaccg agaatcataa aagtggcggt
     4261 acgccggctc cagcattcac cctgattggg gcaatgggcg cttcggcaga cggtgtttaa
     4321 agacaaggcg ccaagcgtat cacgaacttc atccacggca ccgcgctgcg gccacgccgt
     4381 acgtatccag gaagggaatt tctgagagga agtgtttttc tgcatggttc agtatatcac
     4441 gagtgaaaag aagagaaaaa ggactttcat ggttttagaa aagcaacaac cggacacacc
     4501 gcaatagtcc cctgcactga tctggaaatt tctgccggct cttttgcttc acgaaaaaaa
     4561 aagccctgcg tcaataaaaa ccgcagggct ccgttgtttc ccgcaagtaa aagagcgacc
     4621 gcttattcgt tcgcttcaat cgcctcttcg atctctttgt ttacaatagc tgcattcaga
     4681 ctttttgccg catcagtcac tttttgcgga gggatgctct gcagtttggg acgataaata
     4741 ttgaagggga tatacacctc ggggtaatct tccctgttcg ttttcaccac aacccgtttg
     4801 ccttccagcg catcattgcg tggcatattc ttcagcacca gacggtagac attgggtgcc
     4861 gctttaatca gttcgtgctc catggaatcc agagggagaa tcacatccgt aatttcaaat
     4921 tcagaaattc tgcccttggt cagcgaaatc tgctgggttt caacggcccc ttcctcctct
     4981 gtgtagcgca gattcacaag agggggcatg atctgcaatg cgccgatgac ctgcatgctg
     5041 acagtaagcc agataacggg gcgttccgta gaatccgtgc gaatgataat ccggccgttg
     5101 tgatgtccct cgggaagttg tggctgggtt ttaacgacca tctgatattc cttgcccggc
     5161 accacttctt tgacttcctg ctgaatgaaa gtcatgccgt ccacttcagc ggagcgaaca
     5221 gtaaaattca catcctcttt attcgatttg atggttacgg tcgcttcccg gggctcatca
     5281 tcttcaatgc gaccgaagct gacattcatc ggatcaagcg aaatggaggc gatcgcttca
     5341 cctttgatag tcaggcggta attgggattc tcagggtcgt tggaaaagat ggtgatcgct
     5401 ttcgtctgcg gaccctgtcg ccctttcagg ttggtattcg cttgaatgat gagctcttcg
     5461 cccggttcca attctttctt tttaagcgca gtggtggtgc agccgcagga agcccggaca
     5521 ctttcaatct tcagcagaga accgccttca ttacgaatca gaaagccatg ctccaccatt
     5581 tctgagttat ccattttccc gaaatcgtat tccggctcgg gcacgacaag tttaggagcg
     5641 cttccggcag gtgccggcgc agcatcttct gctgtctctg tcgctgccgc tccctcagag
     5701 tccggtgctt cttttacaat ttcgatctgt ggcacttctt tttcagagga agacccggac
     5761 accgccgatt cgggcgcaac tgccttttgt gcagtactct gcggagccgg tgcgggttta
     5821 ggcgcaccac agcctgtaat cagcaccagt gctgccaata caatcatcag ggaatacaaa
     5881 gagcgcataa ctatgtacct ttctttaaac acaggcatac cgcctgcagc gtaaaaccag
     5941 caaagtcgcc cgcacttttt cagcgcaaaa gaccgttgac ataacgttat aactgtactt
     6001 tattgggaca gaaatccgca attttttatc ggggatacac atgactgtgg gcacgctgcg
     6061 tatcgagtcc gaaagcctct gcccggagtg cgcccatttt atccagacag cgctgtgcct
     6121 cctcagcatc tccttttgcc ctataccact cagccatttt ttccatagca ccagcataga
     6181 cataacggtg acctgaagaa aggggacagt atttcagcat ttcttcaaaa gaagcttttg
     6241 ccttttcaag ataacttctg tcgctctctt cgtctttttc tctgccaagt acaagccaga
     6301 ggtaggcatt ccccttgaat ctgtacaagt cggcgtgata cgggttccgc cggagcactt
     6361 cttctgtcca ttccagaaaa tttgcttccc agccgggacg ctgatccgcc acagcatcat
     6421 taaaaactcg ttgggcatat atttcatacc agctggcaat gtggagcgcc aattcagtgg
     6481 aatgcgggaa gcgcctgtcc tgcgcgatca gttcatcaat gcgcttcagt ccggcgggaa
     6541 tcgcccgttt aaacagatac cagggcttcc ggacaatggc aagggcgctt tcaggtacga
     6601 tctcaccctc ctgaatccgg tgaaactttc cgggaacctg cggatccgga cgataaaaag
     6661 caaacgcggt agtcagtact gtctcatctt ctatcagcat ccttgccgcc gaaagaggca
     6721 gccggggcgg ttttttaata tcttctcccc gatcccgtgc cagcgcatag gacggcactt
     6781 cgagcaggaa aaactgaagg ttctggaatc gcatcttctg ctgctcatcc caggacaggt
     6841 tcacaagacg caggcggttg agggcaatat tctgtttgta caggcgaaaa ctggcattgc
     6901 ctgccagaag cagccccaga gcaagcacaa ccgacatgaa ccgctgcata aaaggaagac
     6961 ttggcaaagc ccgttcaccc ggcacttctc cttccgctgt cgccccatgc atcggactgc
     7021 cggaggaaac gcgtccataa aaagcaccgc agaagaaaag cgcaagcatc atcagcgagg
     7081 gatgactgaa attgatatcc agagcagcat ggagcgcaaa agccagaatg cccgcgtaca
     7141 tacccgcaag aatccgcttt tccttcttgt cctgctgacg cagcacactg gcggcacccc
     7201 aaatcacaaa atatgcccag aatgccagga aaagcaaacc accccctagt ccggtttcac
     7261 agaaaaactg aagatagccg ttatgcgcct cccgcacatc accggcaccc ataaactggt
     7321 agacaggata ccctatctga aagttgccca gacctgttcc ccattgccag tttgcaaaaa
     7381 acaggcgggc ggcaacctgc cagtatccga ggcgaaaacc gaaggtggtg gggtcggcaa
     7441 gatcagaagt cgtgacagcc acaccttccg aagtaatcgc cacggaaggt ttgctttcgc
     7501 cagcgctgcc cggctgctgc gcccaggacg gaggggcaca aaaactctgc accagtatga
     7561 gagatataag aagtaccagc gtcagcgtca tcagcagtgc cgctgaccgc ctgaagaagc
     7621 gcggaagccc tgacccggga ttgattttat atagcagaaa actgaagccg gaagatgcga
     7681 gcagccccag aaaggcaccc cgggagtagg taatccataa ggtgtacagc aacagcggaa
     7741 acagcgccag cagcgccgga aaccgaagca atgcccacca gctgctcagc gacgaacagc
     7801 ggcacagcaa agaaaagcag aacagtgccg tggcacatgc cagaatcatg gcaaaaagaa
     7861 tcagcacgga aaggctgtaa taccagggaa ggggtgtttc ctcaagccaa tattcttttg
     7921 gaaagtaagt gacgaataaa aaaaggatga agacaagcat gccaggaatc acggatatgc
     7981 ccagaagcaa cgcccgctcc cggagttctg taaccgtgcg tgccttacgg cgcagttcgc
     8041 gtaaagcctg tttgtaactt ccggcagttg cccagcataa agcaaaagca aaggggatgc
     8101 cgagaataag ataacccgcc agtgcattgg gaaaaagcat cgagccgaaa gcacggttaa
     8161 ccataaagcg gcgcgccagc tcaggtgtga ttacatcggt attgaaatag cgctgcagga
     8221 tagcgggatt ctgaacaatt gtccggagat agggaaggag aaaagaaaaa tgcaggacac
     8281 tgaaaagcgc ctgcgcaccc atagccgcca gaaacagaaa aagaagcagc ttgcggatcc
     8341 ggggcacacc ggtgacatgc agtgaaacgg caaacaaagt cagatagccg acccacagcc
     8401 atacaagcgg ataactctgc ccgtattgcc aggaaaaagg cagcgtgaga aataaataca
     8461 caataagcag tgaaaacacc accagaggcg gcggaaaggt cagcggccgg tctttgccgt
     8521 ggaacacccg aaccagccag atcagagcaa gcagataaat cgcccataca aaatacaggt
     8581 tgtccagcgg aaaggtatag ccgtcgagcc aggtgcgggc aaggataatc agcccgatgc
     8641 cgagggacgg atgaatcaga gaaaaaagaa ccgttccgga cagggcgctg atcacaaaag
     8701 ccatgtcaaa gttgcccggg tcaacctgat caggaaattg aagaccaaga agggcagcaa
     8761 gtgattcttt gactgcggta aaggcgccga aagaggcggc agcacaaagc agcacagtca
     8821 gaatcagcca ggaggaagcc agaaaaagcg cacgggacgg cggggcaaaa tggtcgggcc
     8881 acaggctgcg cagccccata tcggcttcag cttcgccccc tccgccgatc tggataaaac
     8941 gctccgcagg cgcggcttcg gttggaaaag aagatgatat gttttttttc tttttcttgc
     9001 cgatagccat agatttattc cgatccgttg cgcttgcctg cattcatcag caggacaata
     9061 agcaggaaaa taccgagggt ctgcgccaat atgatgccgg tccccatcag agaaagcata
     9121 ggcaacaggg caccgttcaa tccgaccaat gcggcaacca tataaataaa atagacggcc
     9181 atggcagggc gcatgcccac ttccacaagg cgatgtgaaa aatgacgctt gtcgcccagc
     9241 ataatggaat ctccgttgcg ccagcggatc cagacaacgc tcagcgtatc aaagagcggt
     9301 acactcaggg caagtaccgg ggaaacaaca gccgcccggg agtttactcc ctcgatatga
     9361 aaagtgccca ccaccgccac agtggcaaga agatagccgt tgaacatggc accggcatca
     9421 cccatgaaaa ttctggcggg cggaaggttg aagaagagga agccgcccac ggcacccgca
     9481 aaaattacga gcatgaagcg caccatgaag ccggtgtcat aaggctgcac actcaggaaa
     9541 aaagtaatgg cggcaatgat ggacacgccc ccgctcaagc cgtccatgtt gtccagcaaa
     9601 ttcatgctgt tcacaataag cacgacccag agcacggtaa taaaggaaga agaaaacaca
     9661 tcgtgcaggt aaaaaaggtg gatgcgcatg ccgaagaaaa caagtacaag cgctgcggca
     9721 atctgcccga gcagtttgaa ccaggggcgc aggacatgca ggtcatcaac aatgcccaga
     9781 ataaaaatca tggcgcctgc cagcagaata cccaccatct tctgcccgtg atcttcaccg
     9841 agaagcatgg cgagtttctg tgcgagccag gcaggccccc agggccaaaa ccagctcacc
     9901 gccaccacat gaccgataag cacaatatag aatgtgatga aaatcgccgc accgcccaga
     9961 aggggtgtcg atttgtcgtg gctttttctg ccggcaggat gatccagaat gttccagcgg
    10021 cgcgccagct tctgcatgac ccaggtcagt gccagcgaaa gggcaaaaga caggatcagc
    10081 acataggtaa aaactgcata ttgtctggga atcacaaagt caacctgcct tgcagacatg
    10141 aaccggcaac agtgctgcgg gagttaggga caacaatacc acgataaatg ctacaacaac
    10201 accggcacgg gttacaaata aaacaggcat ttccacccgg cctcacagcc cggaagaacg
    10261 ccgccgcgct tccacgaccc tctgataaat ctcatattcc tgacgggcaa tcttttcggc
    10321 agagaatgcg cggaacaccc gatcccgcgc cgcttgcccc agccggtcag ccaattcccg
    10381 atcctgcagc aagcggttca ccgccgctgc cagtgccggc gcatttttgg gcgggacatt
    10441 cagcccggtc ataccgtctt cgttgacaaa ctccacgcct gtgcccagct gtgtggaaac
    10501 caccggggta ccgcaggcgt gcgcctccag aatggaaagc ccgaaagctt cactcctttc
    10561 gctggaagga aaggcaacca ctgccgcggc atggaggtaa ttgaccagat cctcttccga
    10621 cagagcgccc ggaaagagta cttcaccgcc gcagcttcct gccagcgcct cattcgcttt
    10681 gcgttccggc ccatcccctg cgatcacaac ccgcccctgg atcgccgatg ccgcctctat
    10741 cagccagcgc aatcccttgt agtagcggtg tacgccgcag aaaaagacaa aggagccgta
    10801 tttctcccgg agcgcagata ccgcctccgg atcgggggac tggtacgctt ccggcacgat
    10861 ccccagcggc agcacttcgc atttctcacg aaaatgctgc agcgtctccg agctgtccag
    10921 gtactggtgc gacgttggca ggatcaggtc cgcacgtcgc aaaaaggcat gctgaaaagg
    10981 gcggtacaca cgcatcgccc gtgcctgtcg gatgacatcg ctctgatacc ggacaatgag
    11041 ggcggctttc gggcgggcga ggagccagct catttccgct gtgggattgg gaaaatgcac
    11101 gaccaccaca tccgcccggg tcgttcccag ataccaggga aaaagcgggg cgagcggagc
    11161 gctctgaaaa cggcaccact cgcccactgc tgttacggga acatcttcat gaatgaagcg
    11221 acgggtccgc atttcgcgcg aacagaccag cgcttccaca tcacaccact gtctctgata
    11281 ccggcacatg agcgcaacat gacgctccat gccgccgcga accggaggat cataatcctt
    11341 ataaacatga aggactctca aggcaatcct ctgagggtat cacatttgaa taccggtgtt
    11401 tttcgccgca cagactcaga gtgtcatatc atagtgatag acaccttcac gcaattctgt
    11461 ctgaatggtc agaccggagt tgttgaagac agcctgcatg gatttatttt cgtcaagcac
    11521 ttccgctgta aacccggcta taccgtttcg cttcgcaatg gtgcacagct gcttgaaaag
    11581 gaaactgccg atatgcttct gctggtagtc gtcatgaacc acaaaagcaa cttccgcccg
    11641 gttcgttttc aaatccagat agtaaccgcc gatagcgata atcgcttcgc cgtccgcttc
    11701 aggaatagtg gcaacaatgg atacttcgtt gcggtggtcg atatagacaa actgctgcac
    11761 ctgcttgcgg ggcacccatt tcatccgtgc catgaaacgt gtgtacaggg atttctgcga
    11821 cagatcatgg aaaagctcgc gcactttccc ttcatctgtg ggatgaatgg gacgcacggt
    11881 cagctgctgc ccgtctgcca gacgaagcac ggtgcgtgtc tctttgggac ctacagtgat
    11941 tttgcccgta tattcggaaa gttccgcccg gacaaattta ttggcaatcg cctctttcag
    12001 cagttgctcc cggaaatccg gatgggcgat gctgatcagg gcgagggcgc gttcctgaat
    12061 gtttttgccg tgcagatagg caacaccata ttcagtgacc acataatgca catcaccgcg
    12121 ggtcgtcacc acgccggcac cgggcgccag cgttccgaca atacgggatt tgccgtctgt
    12181 tgttgtgctg tacagggcga taatcgcctt gccgtcttcc gcacccgccg caccacggtt
    12241 aaagtcgacc tgcccgccga taccggagta gaactgggtt ccgatggaat cggcgcagac
    12301 ctgcccggtc agatcaactt ccagcgcaac attgatggcg accatcttgt tctgtctgga
    12361 aataataaag gggtcgttaa cgtactcggt ggggttcatg gagaatacgg gattattatc
    12421 gatatagtca tagagccgct tcgtacccat tacaaaactg gcaacgattt taccgcggtc
    12481 catggacttc tggctgccgt taatcacgcc ctcttccaca agttcaataa tggaatcggt
    12541 cagcatttcg gtatgcatgc ccagatcacg ttttttcttc agaaagggtg ccaccgcctg
    12601 cggaacacgt ccgatgccga gctggatggt agagccgtct tcaatcagcg cagcaagata
    12661 ctcacctata tcttccgtac cgggacgcgg ttccggctga atgttttcga gaaccggcaa
    12721 atcaaccgga acgagatagt caatatcgta cacatggatg gtggagttgc cgtgtgtccg
    12781 cggcatttgc ggattcacct gagcaatgac gatgcgcgca ttttccgctg cgcttttgac
    12841 gatgtccacc gatacgccaa gactgcacat accatgctcg tcgggcgggg aaacatgaat
    12901 catcgccaca tccagcggaa gccggcccgt agtaaacaaa cggggaatat cagacaggaa
    12961 aatgggcgta taatcaccca tgccctgctg aatgatttca cgcacattct gcgaaataaa
    13021 gaagctgttg acgggaaaac tgcttgacag ttccttcgtg gcataagggg catcgccccg
    13081 cgtcagcagc tgtacaattt caatgtccgt cagatcgcgc gcacgaccga ccagcgcctc
    13141 aactagcacg gtgggggtgg cacagcctgt tccgagaaaa acacggtcgc cgggttcgat
    13201 agaagcaacg gcttcagcgg cactcacagt aatgtcagcg tattcctgct gccagtcggg
    13261 attgtaactg agtttctttt ccatgttaaa tttactctcc acttaaaatc atgcgcgcat
    13321 cagcaactat cggagcggtg ccgacttcgc cgaccataaa ggggttgata tccatttctt
    13381 ctatttgcgg gaaatcggtc accagctgac tgattcgctg cagggctgcg gcaattgcgt
    13441 tgaggtccac gcctgcctga ccgcgtacgc cacgcagaag cgcaaaagac cgcgtattct
    13501 tcagcatctc cagagcttct tcatgggtaa gcggtgccaa gtagaagctc acgtctttca
    13561 tcacctcaac aaagatgccg ccgagtccga acatgagcat gggcccgaat tgcggatctc
    13621 gatgcatgcc gagaatcact tcgcggccgc ccttgcccat tttttcaacc agcacaccgc
    13681 gtaaatgcgc ctcgggcgca cggcggcgaa tacgaagcat catgagatcg taggcatcct
    13741 gcacctctgc cggcgtatgg agattgagct ttacgccacc gaaatccgat ttatgaagca
    13801 gatctggcga gacgattttc atcactacgg gaaagccgac ccgctgcgct atggcgacgg
    13861 cttcttctcc tgtgcgggcg agtgcgcctg cgggtacatt gaagtcatag gctttcagaa
    13921 tttctttcgc ttcaacctca cccatttcct tgcggccaga ccgttcctga cgggcgaaaa
    13981 tacgctccac acggtgccgg tttaccggga atcgggtcac aatgcgggaa ggtcttccga
    14041 gccaggaggc ataatcgacc atcgacttaa gcgctgcaac ggcacgctcc ggcgaagggt
    14101 agtccggcac acctgccgcc acaaacatgt tgcgcgcttc gtttacctgc gagccgccca
    14161 taaagcatgt gagcacgggc ttgccgtgat gctccgcatc aataatcgcc tgagcggtcg
    14221 cttcacagtc agacatggcc tgcggtgtca gaataaccac ggcgctgtcc acctgaggat
    14281 cagcgagcac ttctttcaca gccatggcat aacgatcgga ctcggcgtca ccaagcacgt
    14341 caatgggatt ggctacgctt gccgccggag ggagtgccgc ttttaccgtg gcagcaattt
    14401 caggcgtaag ggcgtccatg cgcattccca gtgtttccac tgcatccgcc gccataatgc
    14461 cgggaccgcc tgcgttggta atcactgcca cacggttccc cttcggcaga ggctgcatcg
    14521 ccagggctgt ggcataatcg aaaagcgctt cgaaattgtc ggcgcgcacg atgcccgccc
    14581 gggtgaaagc agcgccgtaa gcgatatccg caccggcgag actgcctgta tgagaagctg
    14641 ccgctttgcc gccggcggcg gtagtacccg ccttcagaat gacaacaggt ttttcctgcg
    14701 aaacttcttc cgccacccgt ataaattcgg gaccgctcgt gatgctttcc agataagcgg
    14761 caataacctt ggtctgttta tcctgcgcca gagcacggag cagatcggtt tcgcaaagat
    14821 ccgctttgtt gcctatactc agaagtttgg caagtccgac gccacggcct tcagcccagt
    14881 caagaatggc tgtacagagc gcacccgatt gtgaaagcac ggagatattt ccgggcttcg
    14941 gcatctgggc agcgaaggaa gcattcatgc ggttttccgt gttgatcaga cccaggcagt
    15001 tggggcccag gatacggatg gaatgcttcc ggcaaaatgc caccatttcc ttttctttgg
    15061 cggcgccctc agcacccact tctttgaagc ctgctgtgat caccgtaacc gccttggcgc
    15121 gcaccaccga agcaccggca agggcatcca tcacaaagcg ctgcggaacc acgatcacgc
    15181 aatggtcgat attgatgccg gcgtctcgca catccttata gcagggaaga ccgaaaagct
    15241 catctgtggt tgggttgaca gggattattt tcccctgata tcccccgtta atgaggttgg
    15301 ataaaatggc agaaccgact tttccggggg tgcgggacgc accgatcact gccaccgagt
    15361 ttgggtataa aagtccgtca agcatgttgt tgcacacctg ttgcaggaat actgaaaagt
    15421 cctgcggtaa tgcgccttat caggcgccag cctatagaaa acaactcccc ggcttgcatc
    15481 tcaagcgtag gggtcaatcg tatcccggag ccaattcttt gcgtcacagt tgaggtgaca
    15541 gcaacgtgcg cagggcgggc aatggcggta gaaccgctgc aaacagagac gcggagagcg
    15601 gtgaagcttc tctcttgcga taacgcctct cgctgagttt gcggaaaaaa gtgcgtcgca
    15661 agcaggcggc acgcatacca agccctgcca gaccgtaccc tttcaatgcc ccgatgcatg
    15721 gaaagcggga tccggcaaca tgacagattc caccctgaat gcaaagggaa ggcaataaaa
    15781 gagatggagg cggcacccgg attcgaaccg gggaataaag gctttgcagg cctctgcctt
    15841 accacttggc tatgccgcct ttccag
//
LOCUS       DAOPWB010000077        15016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4051_length_15016_cov_5.441682, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000077 DAOPWB010000000
VERSION     DAOPWB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 15016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15016
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4051_length_15016_cov_5.441682"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKN92_08345"
     CDS             <1..670
                     /locus_tag="PKN92_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNZ48841.1"
                     /translation="MWTEDMRRQIEAEMDLAAFNNYGLSEIIGPGVSGDCAARSGMHI
                     QEDHFLVECLDPESLEPTEDYTYGELVITALTREAMPMIRYRTRDIAALDPCVCECGR
                     TTVRMTRVMGRTDDMLIIRGVNVFPSQIEEALFQVEGTSPHYLVEVNRPDRLDEITVK
                     VELRPEFLSDKMSVMQTLRDSIVRRIQTVAGVHAKVELLAPQTLERSAGKAVRVIDRR
                     NLQG"
     gene            complement(680..2008)
                     /locus_tag="PKN92_08350"
     CDS             complement(680..2008)
                     /locus_tag="PKN92_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ48842.1"
                     /translation="MLKADLHGLRVLFSPAKIQRAVSLGFKTLWQHRLRSFLTMLGIV
                     FGVCSVIAMLAVGEGAGYEAQEQIRQLGSQNIILRSVKPSDTERISSTQSRVSTYGLT
                     YADMTRIQDTIPSVTVLAPGRIIRNHVWNATQRVDAEIVGVTPWYTDVHALHLVSGRF
                     FTPVEMEEHVNVCVITAPVEKRLFPLSPSIGRNVRIDSDYYKVVGVVEAEVSAGSGQS
                     GNGGSGRSNGGYAPRVYIPLSAAKSRFGETLMRRSAGSFEAERVELHEAIIQVDRLED
                     VEETAQIINSLLAVDHKQKDYEMVVPLELLRQAERTKQIFNTVLGAIAAISLLVGGIG
                     IMNIMLASVTERTREIGIRRALGAKKRDIIVQFLIETVILAGTGGAIGVLFGVTIPIL
                     ISHLAEMKTIITPWAPALAFSISAMIGVVFGIYPALRAANMDPVEALRHE"
     gene            complement(2001..2693)
                     /locus_tag="PKN92_08355"
     CDS             complement(2001..2693)
                     /locus_tag="PKN92_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ48843.1"
                     /translation="MSSRPIVSLQQVTKTYVMGEVTLDALHNISFDVHEGEYIAIMGP
                     SGCGKSTLLNILGCLDRPSKGKYLLGGRDVSHFDDNALSEMRGSQLGFIFQSYNLIQQ
                     LDVLENIEVPLYYQGVPAGESRRLATEIAKRVGLGARLHHKPTELSGGQQQRVGIARA
                     LVNNPLVLLADEPTGNLDSSSGAEILALFDELRAQKKTIIMVTHDPDIGLRSDRIIRL
                     RDGCIEEDVRNA"
     gene            complement(2686..4620)
                     /locus_tag="PKN92_08360"
     CDS             complement(2686..4620)
                     /locus_tag="PKN92_08360"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ48844.1"
                     /translation="MLQTTRGFIRRKALIRLGVLILLTVIVLSGAFYFRSGGKKGAPT
                     GATYKAFRGPMEIAVLEGGSIEAKESLQVQCEVQGQTRILKLIEEGYQITQKDVEEGL
                     ILIELDSKDLLDKQLAQELEFQNSSASLTDAIEQYDIQLNQNESDIKAGELLVHFARM
                     DLEKFLGASLTETLLKELGLDAPPDESREKPLDSSTIDFSQYAHPDKLGDGEARQTLR
                     QLENELELAQEDVGLADTKLEGSKRLFDKEFITKTQLDTDQNAYNRKVIALQSAETKL
                     DLFIKYEFVKSAQKYMSEYEESLRKLQRTLKLAHSKEAQSRAQKNSAEARHQLQTRKR
                     EDLATQIEKCIIRAPKTGLVVYGTGEQSFRSETIEEGATVRERQVLITIPDTSVMAVR
                     LQVHESFVKKVKPGQKARIRVDAFPGELLTGEVQRIAVVPDSQNRWLNPDQKVYRTLV
                     NIDGEYDWLKPGMSAEAEIITNVLANALQVPMHAVHLVNDRTVCYVATLFGVETRPVK
                     TGEYNISFIEIQEGLEAGESVLLQPPRAVRAEKEKKEEQKEGSGHLPVAGEEMQQGDS
                     GSGERDASGGDKERGNAESGRRNSENADPGEGRTTEERNREDAGKPGGAARPEAVSGA
                     GRQEGAQRPGGSAGERPAYE"
     gene            complement(4805..5287)
                     /locus_tag="PKN92_08365"
     CDS             complement(4805..5287)
                     /locus_tag="PKN92_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ48845.1"
                     /translation="MQDKLFPGNMLRERRESLGLTIQTTCERTHVPLEYLQAFESGNV
                     EAMPGRAYALGFLRSYCAFLEIEADPYIDQYLLCTQPIKNNGSLQFMKGERFDAILEG
                     SYPRWVQEALAWAIVVLVIILGWFTFTTIVHPFADTGKSAVEASGLEVEEPIHFNEDF
                     "
     gene            complement(5277..8057)
                     /locus_tag="PKN92_08370"
     CDS             complement(5277..8057)
                     /locus_tag="PKN92_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNZ48846.1"
                     /translation="MSTHAEHKQSTGMRWVWGAAFFGIGIILAAAVLCDGFDVQDNLE
                     MGRPADLQGILSVPNEMGAYGALIAGILSILIGDASHLFYMLFFVWALKIVSGGFKGS
                     IFTRITGFILCMGSAVLLCQMIMPTGFSNVDSGGVLGRFLMAPLIGFDLSQGTWILIF
                     SLVATLGFLLCVDLTLVRSFQIIFYILKGCWRVLSWFFQNPGKAGAAKKSIPTEEDLQ
                     PIDSFSADLPEAAFHPEARRERFSFFSWLFLFFLPRLVWVDAPAAAEAGDSGSVFPES
                     VLPDEDALPEEGKEVAGAPVITIHTGSIPVLYPPEEKAEEEDAPPPPPGKEPDCPDSQ
                     KKKKAFPPAPPQGEDVLSEEEEEEEEEEEEQEQEEASDAEYGDVPGSAETPETLQRPD
                     RIIMPYSSPAFLSSAEKEEKVQTPVLRRRAHDYDEVPEDYKYPAHYTRPPIAIFKTAT
                     RSTIPNLNARLQEMALLLEDTLHNHGLEASVIEITRGPTITRFEMEPAPGMRVGRFLA
                     LVDDIALALKAHGVRVQAPIPGKGRVGIEISNEVQDPVVIRELLEHPSFTQGRQVLAL
                     TLGKDVAGVSHVADLEAMPHLLVAGATGSGKTVCIKSLLAGLLFRHTPDELQLMLIDP
                     KMVELSIFNDIPHLITPVVTACDKAAGALGWLINEMEERYRLFADLRVRNIEMYNKSV
                     ENGEIELSGNGSEKGGSLNIVRKLPYIVCVIDELADLMMLARAEVEDAIARLAQLARA
                     VGIHLIVATQRPSVDVLTGVIKANFPARIAFRVSSRVDSRCILDEIGAERLIGKGDML
                     LQQGGNFGQMRLQGAFVSDKEMAALIAYLKLQAPPQYRDEIENYGKTKSSLHDLEDYE
                     DPLLEEAIRVVLEAGQASISMVQRRLRIGYARAARLIDMMELRGIVGPHLGSKPREIL
                     GGALKKEEHHAG"
     gene            8412..9671
                     /locus_tag="PKN92_08375"
     CDS             8412..9671
                     /locus_tag="PKN92_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HNZ48847.1"
                     /translation="MNTATKVKLSAMMFLEFFIWGAWCVTLATYLGTTLKFSGGQIGN
                     AFLTMAIASVISPIFVGMIADKFFPAQLLMGVLHLLGAALMFAAAKVQTPGLFFWVLL
                     GYTLCYMPTLALCNTIAFNQMTNSEKEFPLIRAIGTSGWIVVGLLIGYGLKSTFENIE
                     ASNIPLLIAAGSSLLMGIYSFFLPHTPPQGKGKKVTLGDIFGIEAWALLKDGYFLVFV
                     LASLFISATTSFYINFANPFFNAMSLPYPAALMTSGQISEFLFTALLGFFFATIGVKW
                     MLSLGMLSWGLRYILMMNDAGPGYALWILAIIIHGVCYGFFFTAGQVYVGKVAPKSIQ
                     ASAQGFIALITFGLGQGLGSVISGRIVQHYTVNDVTNWSKVWLVPAIVSIVAGVLFAI
                     FFKVKPAEEICDDAEEIPDAPEAELQS"
     gene            9866..14332
                     /locus_tag="PKN92_08380"
     CDS             9866..14332
                     /locus_tag="PKN92_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48848.1"
                     /translation="MTPRKSRRHLISTGGVLFALLFCCMTVTVADERTVGNSFPGSPL
                     YPESAVMRLDAASDFRDDRNVRCTLIDDAGRYAYLGTWGSPARIIKMDLGEETADLPV
                     QVATLVLEGGEQNISCGMIDSDAGFAWFGTESNPARIIKVSLGEGADPPKLIASVTLP
                     AADRQLRCAAHDKAANMGYFGVNTSPAVVIKVALGEGINPPAKVGDTELPTNGQQLNC
                     SLFDPVRRFAYFSEAASGKLFKIAPGSAGAPPILAGEMSGMPQVRCAVIDPESGHAWF
                     GTNTSPGKVYKVDVSSGSSSPHLLGSITLDSRDENLAAAVIDAPGGFAYFSGEESGIY
                     GAVIKVSLGKNGAPFERIHRQQLYTDEKVLNSGIINVSRGFAWFSSQTAPSRIVKVGL
                     SPQSHITRLGCTELESGEIKTNAAVMDSQAEYAWIGTETSPGRIIKTALYRGEKRPHR
                     LNRIDLAADENTILCGAVDEERGYGWFGTYDGNPGRVVMVSLGEGDAPPERHGALTLN
                     ANESGLRCVALDASREYAFFGTHSQPPRIIKVALGSGSTLPQRAASISFGTLEDAFRC
                     VAYDAVHNLLYFGSDRGYILRVAPGSGNAPPQILDTIQLSSPQSSLVSAVLDPQAGYA
                     WFGTMDNPGRVVKVDIHPQRSFARISTLTLSAGESALFAAARDPQTNYGYFGTNTPPG
                     KVIAVDLGSGSAQPARLGSVTLKEGEGYLRTALLHPNGSRLWFTCNSVPGNLVRLGVG
                     HAEFIKGTRLTLSEPAAVTKVHFYSHRAKGAVRLAIYQYGASPALLWESDAIPNTVQN
                     GWLTVPIASGTPSALALPAGVYWLAYQVNCPDPVPSWSGGAAGSGFSVVHPFGSFPEN
                     LFQNTPTAIQPTSDNWSMYLSYDNHLDAPSDPDSAEITESSIRWIWKDNTANESGFKV
                     WADPGNAIPVTQRETLPADSTSWLWDGLTPNCLYSFQTAAFLFADSEKTQAISRRTKA
                     AAALPGTSILCDHPLDTPFPAGTLFTFTNPAGFGEGIHGDSPDKISAYRYVWNSTADH
                     VFKGTETLWNSGNLIQIPPALSKQYYLHLLSLNEAGEPGGVLHYGPFVIDAEPPQAPS
                     VSGPLLTGNPRPQWSWLSGGGGAGIYEVELNGSGTWTETTNLFYVPDHDLPEGAHTLR
                     VRERDEAGNWSVPGLFTITVDLSALGAAILPLNPPETSASLLSFQIRFSRDVGSSFTL
                     SALSVTGTLAGDLTLTGEGADYLVTLLLDDPEAEGDAGIRISGGMIADGLGNYYHGGV
                     SPLLHIIKWREPYFTETPQGGRKYEGDTHLLRTIPNCTASSLVYKWKCERGDKSIVTG
                     PATSEWLFEPLSLADAGQYWCEVTYDGITRTAEPVLLEVKAPLTITKAPRGASVEAGD
                     SHRFIISVTGGYAPLSYVWYKDGVVLPGDQGDSSLLLTALVPEDSGEYTVSVSDSNGS
                     TVTAPAVLLEIYETTVPLSGAAGTALILLVLFFFVRHRFLSPERRTSADASE"
     gene            14379..14732
                     /locus_tag="PKN92_08385"
     CDS             14379..14732
                     /locus_tag="PKN92_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48849.1"
                     /translation="MKRLLLVALCLCLLSPGTGKIMFPSVEAAESISAPEKHNNEAKN
                     WLCSKCSTKVESPSQPNGSNCPSGGMHRWNNLGEVGDTVYLCDKCSIKVKSKSRPNGS
                     NCPAGGMHHWNKLTN"
     gene            14824..>15016
                     /locus_tag="PKN92_08390"
     CDS             14824..>15016
                     /locus_tag="PKN92_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48850.1"
                     /translation="MKHARARVRMTLALVCVLLFQTAVLFAAPADLSAPLSNWQDLIP
                     GPGVLPGTCTVSLSDSRQSA"
BASE COUNT         3434 a         4287 c         3832 g         3463 t
ORIGIN      
        1 aatgtggaca gaagacatgc gccgccagat cgaggcagaa atggatctcg ccgcattcaa
       61 caactacggt ctgtcggaaa ttatcgggcc cggagtcagc ggcgactgcg ccgcccgcag
      121 cggcatgcat attcaggaag atcattttct ggtggagtgt ctggatccgg aaagcctgga
      181 gccgacagag gactatacat acggagaact ggtcatcaca gcgcttaccc gggaagcgat
      241 gcccatgatc cggtaccgga cccgagatat tgcagcactg gatccctgcg tttgtgaatg
      301 cggccgtacg acagttcgga tgacccgggt gatggggcgc accgatgaca tgctgattat
      361 ccggggggtc aacgtatttc cctcgcagat agaagaggcg ctctttcagg tggaaggcac
      421 ctcaccccat tatctggtgg aagtgaatcg ccctgaccgg ctcgatgaaa tcactgtcaa
      481 agtggaattg cggcctgagt ttttaagcga taaaatgagc gttatgcaga cgctgcgcga
      541 cagcattgtc cggcgcatcc agacggtggc aggcgttcat gcaaaggtgg agcttctggc
      601 accgcaaaca ctggaacgat cggcaggcaa agccgtccgg gtcattgacc ggcgcaatct
      661 gcagggctga ttgccctgct tattcgtggc gcagtgcttc cacaggatcc atattcgccg
      721 cccggagagc cgggtaaatt ccgaagacca cgccgatcat tgctgaaatt gaaaaagcga
      781 gagcaggagc ccagggcgta atgattgtct tcatttccgc cagatggctg atcaggatgg
      841 gaatggtcac accgaaaagc acaccgatgg cacctcctgt gcccgccaga ataacggtct
      901 cgataagaaa ctgaacgata atgtcccgtt tcttggcacc cagagcccgg cgaatgccta
      961 tttcccgggt gcgttcggta acactcgcca gcatgatatt catgattccg attccgccga
     1021 ccagaagtga aatggcggca atggcgccga gcacggtgtt aaaaatctgc ttggttcgct
     1081 ccgcctggcg cagcagttcc agcggcacca ccatttcata gtctttctgc ttatgatcca
     1141 cagcaagaag gctgttaata atctgagccg tttcctctac atcttccagc ctgtccacct
     1201 gaatgatcgc ttcatgcagt tccacccgct ccgcttcgaa agaaccggca ctgcgccgca
     1261 tcagcgtttc tccgaagcgg cttttggctg cactcagcgg gatatagaca cgtggcgcat
     1321 aaccgccatt gctccgaccg gatccgccgt tgccggactg acctgacccg gccgacactt
     1381 ccgcttctac gacaccgaca actttataat aatcggagtc aatccggaca ttccttccga
     1441 tggacgggga cagcggaaaa aggcgttttt ccacgggagc cgtaatcacg cacacattga
     1501 catgttcttc catttccaca ggagtgaaaa aacggccgga caccagatgc agggcatgca
     1561 catccgtgta ccagggcgta acacccacaa tttcggcgtc gacacgctgt gtggcattcc
     1621 agacatgatt gcggataatg cgcccaggcg ccagtacggt gacactcggg atggtatcct
     1681 gaatgcgtgt catatccgca taggtaagcc cgtaggtgga aacacggctc tgcgtggagc
     1741 tgatgcgctc cgtatcagaa ggttttacgc tacgcaggat aatattctgg ctgcccagct
     1801 gacggatctg ctcctgcgct tcatagccgg ctccttcacc cacggcaagc atggcgataa
     1861 cagaacagac gccgaaaaca atgcccagca tcgtcagaaa agagcggagc ctgtgctgcc
     1921 acagagtttt gaatcccaaa gacacggcgc gctgtatttt tgccgggctg aagaggacgc
     1981 gcagcccgtg cagatctgct ttaagcattg cgcacatcct cctcaatgca gccgtccctg
     2041 agcctgataa tcctgtcgga acgcagaccg atatccggat cgtgggttac catgataatg
     2101 gtttttttct gggcgcgcag ttcatcgaaa agcgcaagaa tttcggcacc ggaagaggaa
     2161 tccagattgc ccgtcggttc atcggcgagc aagacaagag gattgttgac aagtgcccgg
     2221 gcaataccca cccgctgctg ctgtccgccg gaaagttctg tcggtttatg atggagccgg
     2281 gcaccaagcc cgacgcgttt tgcaatctcc gtggcgaggc ggcggctttc tcctgccgga
     2341 actccctgat agtacagagg cacttcaata ttttcgagca catccagctg ctggatcagg
     2401 ttgtatgact gaaaaatgaa gcctaattga gaaccccgca tttcggacag ggcgttgtca
     2461 tcgaaatgcg aaacatcccg accgccaaga agatattttc cttttgaggg acggtcaagg
     2521 catccgagaa tattgagcag cgtagacttt ccgcagccgg aagggcccat aatggcaatg
     2581 tattccccct catgaacatc aaaagaaata ttatgcagcg catccaatgt cacctcgccc
     2641 atgacatagg ttttggtcac ctgctgcagc gatacaatag gacggctact cataggcagg
     2701 gcgctctcca gcactcccgc ccggacgttg cgctccttcc tgccgtccgg cacctgatac
     2761 ggcttccgga cgcgctgccc cgcccggttt tcctgcatct tctctgttcc gttcttccgt
     2821 ggtgcgccct tctcccggat cggcattttc gctgtttcgc cgaccgctct ccgcattacc
     2881 gcgctccttg tctccgcctg aagcgtccct ttccccgctg ccggaatcac cctgctgcat
     2941 ctcttctccg gctacaggca gatgcccgga accctccttc tgctcttctt tcttttcttt
     3001 ttccgcccgt acagcccggg gaggctgaag caatacggac tcgcctgctt ccagtccctc
     3061 ctgaatttca ataaaggaaa tattgtactc gcccgttttc accggacggg tttccactcc
     3121 gaaaagcgtg gcaacataac agactgtccg atcattcaca agatgcaccg catgcatggg
     3181 cacctgcagc gcattggcca gcacattggt aataatctca gcttcagcgc tcatacccgg
     3241 cttgagccag tcgtattccc cgtcaatgtt caccagtgtc cggtacacct tctgatcagg
     3301 attgagccac cggttctgcg agtcaggcac aacagcaata cgctgcactt caccggtgag
     3361 gagctcgccg ggaaatgcgt caacacggat tcgcgccttc tgtccgggct tcactttttt
     3421 gacaaaagac tcatgcacct gcaggcgcac tgccatcacc gaggtgtcgg gaatggtaat
     3481 cagcacctgg cgctcccgga cggttgcacc ttcttcaatc gtttccgacc ggaagctctg
     3541 ctcgcctgtc ccgtacacca ccaacccggt tttgggggcg cggatgatgc atttttcgat
     3601 ctgagtcgcc agatcctccc gtttgcgtgt ctgcagctga tggcgtgcct cggcagaatt
     3661 tttctgtgcc cggctctgcg cttctttaga atgagctaat ttgagcgtcc tttggagctt
     3721 gcgcaaagac tcctcatatt cagacatgta tttctgcgcc gacttcacaa actcatattt
     3781 aataaacaga tccaatttgg tttccgccga ttgcagagcg atcactttgc ggttgtaggc
     3841 gttctgatcg gtgtccagtt gggtcttggt tatgaattct ttatcaaaaa gccgttttga
     3901 cccttccagt tttgtatccg ccagccccac atcctcctgc gcaagttcca gctcattttc
     3961 cagctggcgc agcgtttgac gtgcttcacc atctccgagc ttatccggat gggcatactg
     4021 tgagaaatcg atggttgagg aatcaagcgg tttttcacga gattcatcgg gcggcgcatc
     4081 aagcccgagt tctttcagca gggtttcggt gaggctggct ccgagaaatt tttccagatc
     4141 catgcgggca aaatgaacca gcagctcccc tgctttaatg tcgctttcat tctggttcaa
     4201 ctggatgtcg tactgttcga tggcatcggt gagagacgcg gaagaattct gaaactccag
     4261 ttcctgggca agctgtttgt ccagcaggtc cttggaatcg agctctatca ggatcagacc
     4321 ttcctccacg tctttctgag taatctggta cccctcttca atcagtttga ggatacgtgt
     4381 ttgaccctgc acctcgcatt gcacctgaag ggactctttt gcctcaatgc tgcccccttc
     4441 cagcacggcg atttccatgg gaccgcggaa ggctttgtaa gtggcacctg tgggagcccc
     4501 ttttttgccg cctgagcgga aatagaacgc accgctcagc acaataacag tcagaagtat
     4561 caggacgccc aggcggatga gagccttgcg ccgaataaaa ccgcgggtgg tctgcagcat
     4621 attacctatc ctttttcaca gccggaacac attacgccat gcaaaaggaa gaccgtcggt
     4681 tccgggacgc ataccgtgct tttacaacac ggaagaaggc cgcctccacg cagttaccgg
     4741 cgcagacccg gaagcagaaa ggaggaattt gcggataaac tccggagaaa ggatggaagt
     4801 cccgttagaa gtcttcatta aaatgaatgg gctcctccac ttccaagcca cttgcctcta
     4861 ccgcactttt cccggtatcg gcgaaggggt gcacaatcgt agtgaaggtg aaccagccga
     4921 gaatgattac gagaacaaca atcgcccagg ccagtgcttc ctgcacccac cggggataac
     4981 tgccctccag aatagcatcg aaccgttccc ctttcataaa ctgaagggaa ccgttatttt
     5041 ttatcggctg cgtgcacagc agatactggt cgatataggg gtcggcttcg atttccagaa
     5101 aagcgcaata gcttcgcaga aaaccgagcg cataggcacg tccgggcatg gcttccacat
     5161 tgcccgattc aaatgcctgc aagtattcca gcggaacatg ggtgcgttca catgtggtct
     5221 gaatcgtaag cccaagtgat tcacgacgct cacgcagcat gttcccagga aaaagcttat
     5281 cctgcatggt gctcttcctt ttttagtgcg cctccgagta tttcccgggg cttactccca
     5341 agatgcggcc cgacaatacc tctcagctcc atcatgtcaa tcagccgcgc tgctctagca
     5401 taccctatcc gaagcctccg ttgcaccatt gatatcgaag cctgcccagc ttccagcaca
     5461 acccgaatgg cctcttccaa cagaggatcc tcgtaatctt ccaaatcgtg cagcgaactc
     5521 ttcgtttttc cgtaattttc aatctcatca cgatattgag gcggcgcctg caatttcaaa
     5581 taggcaatga gcgcagccat ctctttgtcg ctcacaaatg caccctggag gcgcatctgc
     5641 ccgaaattgc cgccctgttg cagcagcata tcgcctttgc ctataaggcg ttccgcacca
     5701 atttcatcga gaatgcaccg ggagtccaca cgggacgaca cccggaaagc gatgcgagcg
     5761 ggaaaattcg ctttgatgac gccggtcagc acatccacag acggacgctg ggttgcgaca
     5821 atcaggtgaa tgcctacggc acgggcaagc tgcgccagcc gggcgatagc atcctccacc
     5881 tcggcacggg caagcatcat cagatctgcc agctcatcaa taacacacac aatataaggg
     5941 agcttgcgaa cgatattaag agagccgcct ttttccgaac cgttaccgct cagttcgatc
     6001 tcgccgttct cgacgctctt attgtacatc tctatattcc gtacacgcaa gtcggcaaaa
     6061 agacggtacc gttcttccat ttcgttaatg agccagccca gtgcacccgc cgccttatcg
     6121 caggcggtta ctacgggggt aatcagatga ggaatatcat taaagatgga gagttccacc
     6181 attttcggat caatcagcat caattggagt tcatccggtg tatggcggaa cagcaagccc
     6241 gccagcagcg atttgatgca tactgttttc cccgaacctg tagcgcccgc caccagcaga
     6301 tgcggcatag cctccagatc agcgacatgt gacacaccgg caacatcttt gcccaatgta
     6361 agggcaagca cttgtctgcc ctgagtaaaa gaaggatgtt ccagcagctc acggataacg
     6421 accgggtcct gtacttcatt ggaaatctcg atgccgaccc tgcctttccc gggaatgggc
     6481 gcttgtacgc ggacaccgtg cgctttgagt gccagcgcaa tatcatccac cagggcaaga
     6541 aaacggccga cccgcatacc cggagcgggt tccatctcaa agcgggtaat cgtgggaccc
     6601 cgggtaattt cgattactga cgcctcgagg ccatgattat gcagcgtatc ttcaaggagc
     6661 agcgccattt cctgcagacg ggcattcaga ttgggaatcg tcgatcgtgt cgctgtttta
     6721 aaaatggcaa tgggcgggcg ggtataatgc gccggatatt tatagtcttc aggcacttca
     6781 tcgtagtcat gggcacggcg gcgcagaaca ggtgtctgta ctttctcttc tttttcagca
     6841 gatgataaaa aagcgggtga agaataaggc atgattatcc ggtcgggccg ttgcagcgtt
     6901 tccggagtct cagcagagcc gggcacatct ccatattctg catcagatgc ttcctcctgc
     6961 tcctgctctt cttcttcctc ttcctcttcc tcttcctctt ctgaaagaac atcttcgccc
     7021 tgcggaggag ccgggggaaa cgctttcttc ttcttctgag aatcagggca gtcgggctct
     7081 ttacctggag gcggcggagg ggcatcttct tcttccgcct tttcttccgg cggatacaac
     7141 acagggatag aacctgtatg gatcgtaatt acaggggcac ccgcaacctc ttttccttct
     7201 tcaggaagtg catcttcatc agggagaaca gactcgggaa aaacagatcc ggaatcacct
     7261 gcctcagctg ctgcaggcgc atcaacccat acgaggcggg gcagaaagaa aagaaatagc
     7321 catgagaaga aagaaaacct ctctcttctt gcttccggat gaaaagcagc ttcgggcagg
     7381 tctgctgaaa aactgtcgat gggctgaaga tcttcttctg tcggaatcga tttttttgct
     7441 gcgcccgctt tgcccggatt ctgaaaaaac cagctcagca cgcgccagca tccttttaaa
     7501 atataaaata ttatttggaa gcttcggacc agggtcaaat caacacaaag aagaaaaccc
     7561 agcgtcgcca caagtgaaaa aatgagaatc cacgttccct gcgacagatc aaacccgatt
     7621 agcggcgcca tcaaaaacct gcccagaaca ccacccgaat cgacattgga aaaacctgtc
     7681 ggcataatca tctggcaaag caggacagcg gatcccatgc acaggataaa tcctgttatc
     7741 cgggtaaaaa tactgccttt gaacccgccg gagactatct tcagtgccca aacaaaaaag
     7801 agcatgtaaa acaggtgaga agcatctccg atcaggatgg acagtatacc ggcgatgagg
     7861 gcgccgtatg cgcccatttc gttgggtaca gacaggatac cctgaagatc tgcgggccgt
     7921 cccatctcca gattatcctg cacatcaaag ccatcacaca atactgcagc tgcaaggata
     7981 atcccgatac caaaaaaagc agctccccag acccaacgca ttccggtcga ctgtttatgc
     8041 tctgcatggg tgctcaacaa aacaactccc ttctttcttg atcctgtggt cattttagca
     8101 gatgttttgc taaatttcaa ccaaatattg tggttgaacc caggtgtcgt aacaatatat
     8161 agacataaaa gcggacgttt cgccgtatct gatcacgccc gcaatacatc aaaacaagca
     8221 gggaccaggc agccttttct taaaaaaaca ccaaatctgc atgcctgcaa attctgaatt
     8281 ttcgtgaaag gcaaaggctc ttttatttcc ggtaatcaaa agaatggtct ttcttgaaat
     8341 aaacttgcgg gaacggtaga ctttagaaga tgttttaccc tattcaccaa tccaaacggg
     8401 agagagtacg tatgaataca gccactaaag tcaaactgag tgccatgatg tttctggagt
     8461 tttttatctg gggagcctgg tgcgtcaccc tcgccaccta tctgggcacc accctgaaat
     8521 tctccggcgg ccagatcggc aatgcctttc ttaccatggc gattgcgtcg gtcatatccc
     8581 ctattttcgt gggcatgatt gccgacaagt ttttccctgc ccagctgctg atgggcgtgc
     8641 tgcatctgct gggcgccgca ctgatgttcg ctgccgccaa agtgcagacc ccaggtcttt
     8701 tcttctgggt actgctcggc tacaccctct gctacatgcc gaccctggcg ctgtgcaaca
     8761 ccattgcctt caaccagatg acgaattcgg aaaaagaatt tccgctgatc cgcgccattg
     8821 gtacgagtgg ctggatcgtg gttggcctcc tgatcggtta tggtctgaaa tcaacttttg
     8881 agaatattga agcctccaat attccgctgc tcatcgccgc cggttcctcg ctgcttatgg
     8941 gcatttattc cttcttcctg ccccataccc cgccgcaggg taaaggcaaa aaagtcacgc
     9001 tgggcgacat cttcggtatt gaagcctggg cgcttctgaa agacgggtat ttcctcgtat
     9061 ttgtgctggc ttctcttttt atcagtgcca ccacttcttt ttatatcaat tttgccaatc
     9121 ccttctttaa cgccatgagt ctgccctacc cggcagcgct gatgacaagc ggtcagattt
     9181 ctgaattcct ctttacagcg cttctcggct ttttcttcgc cactatcggt gtgaaatgga
     9241 tgctcagtct gggcatgctt tcctggggtc tgcgatacat cctcatgatg aatgatgcgg
     9301 gcccgggcta tgcgctctgg attcttgcca tcatcatcca cggtgtctgc tacggcttct
     9361 tcttcaccgc cggtcaggtc tatgtgggga aagtggcgcc taaatcaatt caggcaagcg
     9421 cccagggatt tattgcgctc atcaccttcg ggctcggtca ggggctcggc tctgtcattt
     9481 ccggccgcat cgtgcagcat tatacggtga acgatgtaac aaactggtct aaagtctggc
     9541 ttgtgcctgc cattgtctct attgttgccg gcgttctgtt cgctatcttc ttcaaagtaa
     9601 aaccggcaga agaaatatgt gatgacgctg aagaaattcc tgatgcgccg gaagccgaac
     9661 tgcagagctg atcttttgca gacctgcacg tgtttgcaga cggcagcgtt gccgcttcct
     9721 cagattccat actgctgatt gtgccggctg gacgcatgcg ctgcatccag ccgttttttg
     9781 tttagtccgt ttttccgaaa atttgcgggg atttcccggc tgcaatgcat ctgtactctt
     9841 cttaaaaaaa atcgggaggg gaaccatgac gcctcgtaaa agcaggagac atctgatcag
     9901 taccggagga gtgctcttcg ctctcctctt ttgctgcatg actgtaactg tcgccgacga
     9961 gcgaactgtc ggaaactctt ttcccggttc gcccctttat cccgaaagtg cggtgatgcg
    10021 gctggatgcc gcttcggatt tccgggacga ccgcaatgtg cgatgcactc tcatcgacga
    10081 tgccgggcgg tatgcttatc tcggcacctg gggcagccct gcacggatca tcaaaatgga
    10141 tctgggcgaa gagactgcag atctgcctgt acaggtggca acactggtgc ttgaaggagg
    10201 cgaacaaaat atttcctgcg gcatgattga ttccgatgcc ggcttcgcct ggtttggaac
    10261 ggaaagtaat ccggcccgaa tcatcaaagt atccctggga gaaggggccg acccgccaaa
    10321 acttatcgca tctgtcacac ttcccgccgc tgaccggcag ctccggtgtg ccgctcatga
    10381 caaagcggca aacatgggct atttcggcgt caacaccagc cccgccgtag tgatcaaagt
    10441 cgccctgggc gaagggatca accctccggc aaaagtgggc gacacagagc tgccgacaaa
    10501 cggtcaacag ctgaattgca gtctcttcga ccctgtccgg cgctttgcct atttttccga
    10561 ggctgcatcc gggaagctct tcaaaattgc accgggttcg gccggagcac caccgatact
    10621 ggcaggcgaa atgagcggaa tgcctcaggt gcgctgcgct gtaatcgacc cggagagcgg
    10681 gcatgcctgg ttcggaacca acacgtcccc gggcaaggtc tataaagtcg atgtaagcag
    10741 cggctcttca agcccgcatc ttctaggctc gatcacattg gacagcagag acgagaattt
    10801 ggctgcggca gtgatcgatg cgcccggggg ctttgcgtat ttcagcggtg aagaatccgg
    10861 catttacggt gctgtgatca aagtgtcgct aggaaaaaac ggcgcacctt ttgaacgcat
    10921 acaccgccag cagctgtaca cagacgaaaa agtgctcaac agcggcatca taaacgtgag
    10981 ccggggcttt gcctggtttt catctcagac cgcaccatca cgcattgtta aagtcggatt
    11041 gtctccgcaa agccatatca cccggctggg ctgcaccgaa ctggaaagcg gcgaaataaa
    11101 gaccaatgca gccgtcatgg attcacaggc tgaatatgcc tggataggta ctgaaacatc
    11161 accgggcaga attatcaaga ccgcattgta tcggggcgaa aaacgacctc accggctgaa
    11221 ccgtatcgat ctggcggcgg atgaaaatac gattctgtgc ggtgccgtag acgaagagcg
    11281 gggctatggc tggttcggca cttacgacgg aaaccccgga agagtcgtca tggtttcgct
    11341 gggtgagggc gatgcacctc cggagcggca tggcgcactt acgctgaacg ctaatgaatc
    11401 cgggctgcgc tgtgtagctc tggatgcatc ccgtgaatat gcttttttcg gaacccattc
    11461 tcagccgccc cgcatcatta aagtggcgct gggatccggc agtactcttc cgcagcgcgc
    11521 tgcatccatc agtttcggta cactcgaaga tgcattccgc tgtgtcgcct atgacgctgt
    11581 ccataatctg ctgtatttcg gttctgatcg gggatatatc cttcgggtgg cacccggttc
    11641 cggcaatgcg ccgccccaga ttctggacac catccagttg tcatcgccgc aaagttctct
    11701 tgtcagtgcc gtactggatc cgcaagccgg ttacgcctgg tttggtacga tggacaatcc
    11761 gggccgtgtc gtcaaggtgg atatccatcc gcagcgcagc ttcgcccgca tcagcacgct
    11821 tacgctgagc gccggtgagt cggcgctttt tgccgccgcc cgggatccgc agaccaatta
    11881 cggctacttc ggtaccaata caccgcccgg taaagtcatc gctgtagatc tgggctctgg
    11941 cagcgcacag cctgcccgcc tcgggagtgt aaccctgaaa gaaggggaag ggtatctccg
    12001 caccgccctg ctgcatccga acggttccag gctctggttt acctgcaaca gcgtcccggg
    12061 gaatctggtg cgcctcggtg tcggtcatgc tgaatttatc aaaggaaccc gcctgacact
    12121 gtccgaacct gctgctgtca caaaagttca cttctactcc catcgggcta aaggcgccgt
    12181 gaggcttgca atctatcagt acggagcctc ccccgcgctt ctgtgggaat cagacgctat
    12241 ccccaatact gttcaaaatg gctggctgac cgtgcccata gcctcgggta cgccctctgc
    12301 tctggcatta cctgccggtg tttactggct cgcctatcag gtgaactgcc ccgatcctgt
    12361 gccgagctgg tccggaggcg cggcgggcag tggcttttcc gttgtccatc ccttcggctc
    12421 ttttccggaa aatctgtttc aaaacacgcc taccgccata cagccgacaa gtgacaactg
    12481 gagcatgtat ctttcctatg acaaccacct tgacgctccc tccgacccgg acagcgcaga
    12541 gattacggaa agcagcatcc gttggatctg gaaagacaac acagccaacg aaagcggctt
    12601 caaggtctgg gcagatccgg gaaacgccat tcccgtgacc cagcgtgaaa cactgcctgc
    12661 ggacagcaca tcctggctct gggacggact gactccgaac tgcctttaca gttttcagac
    12721 agcggcattt ctctttgcgg acagcgagaa aacccaggcc atttccaggc gcacgaaagc
    12781 agccgctgct cttccgggaa cctctattct ctgtgatcac ccgctcgata cgcccttccc
    12841 cgcaggaact ctttttacct tcaccaatcc cgccggattc ggagagggaa tccatggcga
    12901 cagtcccgac aaaatcagcg cataccgcta tgtatggaac agcacagccg accatgtttt
    12961 caagggcaca gaaaccctgt ggaacagcgg caacctgatc cagataccgc ctgccctctc
    13021 aaaacagtat tatctgcatc tgctgtctct caatgaggca ggcgaacccg gcggagtgct
    13081 gcattacggc cccttcgtca ttgatgcgga accgcctcaa gccccttctg tctccgggcc
    13141 tctgctgacc ggcaacccgc gccctcaatg gagctggctc tccggcggag gcggagcagg
    13201 tatctatgaa gtggagctga acggctcagg aacctggaca gaaacaacta atctctttta
    13261 tgttcccgat cacgatcttc ccgaaggagc tcataccctg cgggtgcgcg agcgggacga
    13321 ggcaggaaac tggtcggtgc ccggtctctt taccatcacc gtggatctga gcgcacttgg
    13381 cgctgccatt cttccgctga acccgccgga aaccagtgct tcccttctgt cttttcagat
    13441 acgcttcagc cgtgatgtgg gcagcagttt cacgctgtcc gcgctttcag tgacagggac
    13501 gctcgccgga gacctgacac tcacaggcga aggagccgac tatcttgtta ctctgctgct
    13561 ggatgacccc gaggcggaag gagacgcagg catccgtatc tccggcggca tgatcgccga
    13621 cggactgggc aattactatc acggcggcgt ttcaccgctc ctgcacatca taaaatggcg
    13681 cgaaccctat ttcacggaaa ctccgcaagg tgggcgcaaa tacgaaggcg acacccattt
    13741 gctgcgtaca attcccaact gcaccgcctc ctcactcgta tacaaatgga agtgcgagcg
    13801 aggggataaa tcaatcgtca ccgggcctgc cacgtctgaa tggctctttg agccgctcag
    13861 ccttgccgat gccgggcagt actggtgcga agtcacctat gacggaatta cccgaacagc
    13921 agaacccgtg ctgctggaag taaaagcccc tctgacgata accaaagcgc cccggggcgc
    13981 atccgttgaa gcaggtgaca gccaccgctt tatcatttcc gtaacaggcg gctatgcccc
    14041 tttgagctat gtctggtata aagacggcgt ggtgctgccg ggcgatcagg gagacagcag
    14101 tctgctcctg acagcgcttg tcccggaaga cagcggagaa tatactgttt ccgtaagcga
    14161 cagcaacgga agcactgtta cggctccggc tgtactgctg gaaatatacg agacgacagt
    14221 ccccctttca ggtgcggcgg gaacggcact gattcttctt gtgctgttct tttttgtccg
    14281 ccaccggttt ttatctcctg aacggcggac atcagccgat gcaagtgaat aaaaaacaga
    14341 aaacagcgtc ttagtcagaa acacaggagg cttctgccat gaaacgactg cttttagttg
    14401 ccttatgcct ttgtctgctc tcaccgggta cagggaaaat tatgtttcct tccgtggaag
    14461 ccgctgaatc gatttcggca ccggaaaagc ataacaatga agccaaaaat tggctctgca
    14521 gcaaatgcag caccaaagtc gaatcgccct cccagcccaa tggaagcaat tgcccctctg
    14581 gcggcatgca tcgctggaat aatctgggag aggtcggtga cacagtctat ctctgcgaca
    14641 aatgcagcat caaggtaaaa tccaaaagca gacctaacgg cagcaactgc cccgcaggag
    14701 gcatgcacca ctggaacaaa ctgacaaatt aattttgttc gggctgcagc aatagcattt
    14761 cttcgtgcgc accggatata atagcgcatg cttcgcactg tttttataaa aaaatatacc
    14821 cgcatgaaac atgcacgtgc ccgtgtccgg atgacgctgg cacttgtctg tgtgctcctt
    14881 tttcaaaccg cagtgctctt cgcagcgcca gcagatcttt ccgctccgct ttccaactgg
    14941 caggacctga ttccggggcc cggtgtgctg cccggcacat gtactgtcag tctttccgat
    15001 tctcgacagt ccgccg
//
LOCUS       DAOPWB010000078        15001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4061_length_15001_cov_4.745818, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000078 DAOPWB010000000
VERSION     DAOPWB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 15001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15001
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4061_length_15001_cov_4.745818"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKN92_08395"
     CDS             <1..369
                     /locus_tag="PKN92_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48851.1"
                     /translation="RSREETAATYNYQPAQGEMLTLERSEILPATVRPGENADASIQY
                     ALLGTGGSIQVSEQRSLLQNGALVADVSSHSFTREDGTWVSTQPFRVPSNLRPGTYTI
                     VTTVRTARSAISGQASFTVL"
     gene            525..1277
                     /locus_tag="PKN92_08400"
     CDS             525..1277
                     /locus_tag="PKN92_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNZ48852.1"
                     /translation="MKKRFYFPVLLLTCLALIHGCATCKNEITGQTAQSFKSVVQKEV
                     EIHYLLHLPEGYENSLEPAPLLLFLHGAGERGSDLEKVARHGPPALIARGEWNQPFIV
                     VSPQCPADAWWTDGLQTDTLEALLDHIEKNYRVDKNRIYVTGLSMGGFGTWRLATENP
                     QRFAAIAPICGGGDPFHAKRIKELPVWVFHGAKDDVVPILSSEIMVNALKQVGNDVKF
                     TVYPDTGHDSWVAAYDTPELYEWLLQQKRSGK"
     gene            1442..4162
                     /locus_tag="PKN92_08405"
     CDS             1442..4162
                     /locus_tag="PKN92_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48853.1"
                     /translation="MENKAGTTRIDLKVLWQALTDEEKERACQDFWEDPDRGTDLIHY
                     VYDHLSQILHFREKYLKKRSLEEKTRFLFKFILRPSLEPALDAALRSWLLAHHRSMIC
                     SFLDSLGIPHENGLINDDPSPPDSQAFSACIQKLLSAYEPRIVCIYIGYLVLTGDDTP
                     WATLPKAVEELPLDFAEHLSQKIGTQEVVEPTVEEEEKEPERTSSFTTLDHLLIKTMV
                     ASALQVDHALPEEHVEHLIDEVVELNVERKHSFFHRGFFNALFNREMAFDFPGANDER
                     RSWYFCGAFMGYLRGTDKEKCRNLLINQISIVQELVNNRALECGAMLLPYVYPILLKD
                     RKFDLLKIWLLQHIFGLWPTERERFLLHVYHDAARMLRRGASAEAGLLLETLNKIVSK
                     YEKLDSDFVNQIEPLIARKQGQASQLEGKFAAAQTLFSKALEKLDKENAANTLADLGL
                     VSGGFRSLKSILPAGNMADTKGIANSLAKGKDFYLQAIDQYGTLATNAHFCMGILYFV
                     GAEQCEEKCAHHLQIALDGMLQKHKIYNENGLVDWTRFLLGIALLEVMDPANLQNAIE
                     SIQHNLENKIQYPMWLWEKVLESLKAFDVPDTIEDVAHRLFQKRNNEAFQTLWSTGFY
                     TIGAVQKPFFEWIKKKKEATFEKWGDLETLFHAARKQEAIELCETILDFMEIEAQAEQ
                     HCRLLFIEFLEKNPLLQPIWNESDINALLVRLYELNGNFIDAAPKLRSAFFQCRDGGE
                     AHQIQEARQIIQTLQDYGLGEDYWKDLHQCLSGLEQKQIQMQETENTLRAGSVIHVLY
                     IGGNEIQTAYKDSIQEEIRQNYPGLKCKFLFPGWNTNWQKDFDEAKPYIAEANVVVIN
                     KLIRTHLGRALRKHCGSSHPWISCCGRGRHSIQNRIIEAAQWACTLNKYN"
     gene            4407..4763
                     /locus_tag="PKN92_08410"
     CDS             4407..4763
                     /locus_tag="PKN92_08410"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNZ48854.1"
                     /translation="MMVLYAWVWLRLRPKQFIVHQDRLDILWPLKRVQIPRTDISSVR
                     LISRENLKNEISRGVRIGAGGLWGGFGWLWTRRRGVIQMYISRTDNFVWIECAGARPW
                     LITPEQPEAFVRALSR"
     gene            complement(4946..5956)
                     /gene="cydB"
                     /locus_tag="PKN92_08415"
     CDS             complement(4946..5956)
                     /gene="cydB"
                     /locus_tag="PKN92_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNZ48855.1"
                     /translation="MDLNTSWYILIGVLFGGYAVLDGFDLGAGILSLFSRDEKERSTC
                     MSAIAPVWDGNEVWLLAGGGALFAAFPKVYAAVFSGFYLALMLVLYALILRAVSMEFS
                     AHVHSPAAKKIWGIVFGASSLIVALLLGVAFANIMRGLPISAEGDYTGGFFGLLNPTG
                     LIGGVVGIAFFTMHGAAYMSMKTDGALQQRMLGHGKKMAVASAVVFVLLLVMLGLQAK
                     HLFSGGLFYWLAILVMAGGFVLSIRSASGQKSGLFFIGSALVILAYIASVAVGLYPNM
                     ALSSLDPAWSLTAYNASSTPRTLQAMLIIALVGMPVVLAYTVVIYRVFRGKASDSYYS
                     EN"
     gene            complement(5959..7290)
                     /locus_tag="PKN92_08420"
     CDS             complement(5959..7290)
                     /locus_tag="PKN92_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNZ48856.1"
                     /translation="MELDPILLARLQFAATICFHFIFPPITIGLAWLLVILETGSWKT
                     GKEVYARAAAFFARLLALTFTLGVATGITMEFQFGTNWAEYSKFVGDIFGAPLAAEAV
                     LAFFLESAFLGLYLFGRGRISAAAQCFSAFMVALGSTLSAFWILVANSWQQTPSGYVL
                     QNGRAELTSFWEAVTSPSMLPRFFHSFTAAVITGAFFMAGVSAWLLIKNSKNEKARLT
                     VTLALIVGFIAAAAELFPFGHWHAVQVAEHQPAKLASFEGLMEGQTRAPLLLFGIPVG
                     DKIIGEVRAPGLLSLLVGGSLDTHVPGRNDFPPEDLPQLFLPFTAFHLMVGLGTFFIA
                     LTGWGVLQLWRRKLFEQRWFLYCLVLAAPLPVFTNQLGWIAAEVGRQPWIVYGVLRTS
                     DAISRTVPGAHILFSLIMFTALYTLLLVAYLWFLARMVQKEAALFPAKEGD"
     gene            7841..10081
                     /locus_tag="PKN92_08425"
     CDS             7841..10081
                     /locus_tag="PKN92_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ48857.1"
                     /translation="MQQPSCEQIAEFINSNLDTIVQDCSAQITPDQTGKLRDLVADWV
                     RNLLNSLQGKSYRAGEWASHFSSTAREMGLSSPEAFTLLDKFRISLTGACKQFQPQHT
                     ETEIRICLFEASEIFKNHLVEYYLHQADEEAAAGLRKQKAALEASASPMAVLDEQGRI
                     EIANREMGRLLKTGSDALTGTDFYSYCTEETAALLRASQRKKTSSQQPLTVDGSLKFT
                     RTELNSRFNLRPVFDTAGHRSGTVVCLLPESRPTENVEVGIQYLEDHILTLLSLPVQV
                     VAADHTILYNSEELLKLSLPGYDSSQPICCYLKQHQRGSEDYWFCKTLFEQGHFHWEE
                     IHFECDGETRWFHMLNFPLTDGTGSIVRMVTCVYDTTRRRQIQKQMESHLIQQQRSSL
                     VSQIAVTVAHQLRNPLSVVLGFAEMLSKGLPPDQYSEAVNRIHRNSLRCKDIVENLLD
                     FGKGTPLERRPVDFENLIQKTVRIRLTPAQDRLIEWRFSGKPTPVECLPEQLAQVVHS
                     LLQNALKVAHTRILFSVEVKGYFVRLRIVDDGPGIPISDRERVFEPFYTSHRKEGAVG
                     LGLSLARALANDYGGSLTISTPGAEEPQGACFIFQLPLLSEPNHPEEKQAPETNISGN
                     EHQILIVDDNADLRDLLTTTLYNRGFQVTTVTSGMAGLEKLKQQHYDAIILDFLLEGS
                     LTGRQIYEEIQASHPEFADKVLFITGDMLTYQTRLYLESTHRPVLEKPFLMTDFLSAI
                     AKIIQD"
     gene            10133..11260
                     /locus_tag="PKN92_08430"
     CDS             10133..11260
                     /locus_tag="PKN92_08430"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNZ48858.1"
                     /translation="MSSPMRILFLALTSAVASFSLLAEEQRIPAEKVLALTPSETNPR
                     NSEGDFIALADGTLLFIYTRFSSGADDFDAACLASRRSEDGGATWSTEDEIVLENEGQ
                     RNVMSVSLLRLESGEIALFYLRKNGAQDCRPCMRVSRDEGRSWSDAVECIPEPVGYYV
                     VNNSRVIQLRSGRLVIPAAIHALHGAPFKERSTAVCFLSDDAGKNWYRSDTELTAPEQ
                     ARAGFQEPGIVELPDGTLLMHLRNDLGVFYESRSGDGGTTWSDAVPTTLKTPVSPGTC
                     VLIPGSERLLLLWNDHSQIEEALAGKRTPLTLAVSDDGGRHWHSTRLIEDNPAGWYCY
                     TAARFTKTHLLLGYCAGDTRSMPGLSLTQITRIALTDLAAL"
     gene            complement(11273..12598)
                     /locus_tag="PKN92_08435"
     CDS             complement(11273..12598)
                     /locus_tag="PKN92_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ48859.1"
                     /translation="MEKKHTHTEATRRDFLKTTGALGAGLYLGGAMTSGNAQAAEALA
                     LKGGAKAVTASAEGSTRWPIYGSEEIEAVTALLKDPGYGPIAEFEEAWKVFHDCPFTK
                     AHFNGTSALTSMLFALDLPAGSEVLVANYSTWFPVVPMRFFDLVPVFVDVNPYTMNLD
                     LEDCAKRLTGKTRAILAVHWFGLPCEMDDITAFAKEHDLIVLEDASHAHGAKVNGVRI
                     GNWGRMAGFSLQGSKPLPAIEGGMAMYKDRGDFERAVTYGNYELPNTFPDDSPYRKYQ
                     GTAFGSKLRIHPVAAILGKFQLEKLEERNAAGKAQMKKLNDRITQLPGLTTQYVREGI
                     ERVFYSKNMLFIDEAKAGMSRENAIAALQAEGVDVTAFTWTLLHTYPIFGESKYWRQM
                     PVLPDAVPGCDQANRQAIQLPYWTSDQPELVEQYGDAFEKVWANRKSLA"
     gene            12829..14304
                     /gene="glgA"
                     /locus_tag="PKN92_08440"
     CDS             12829..14304
                     /gene="glgA"
                     /locus_tag="PKN92_08440"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNZ48860.1"
                     /translation="MNKSLRILFVASELTPLMSTGGLAEVAAALPPALQHAGHDVRIA
                     LPCYRQIPQEARGVVITACEASADGYARRGALRRTVVPDTSIPLYLVEHEDFFGRDGA
                     YGSGSYEYTDNGERFSFFCRALLDALEKIDWQPDIIHCNDWQTAALPIFLKTQLQARP
                     FWQRTACLLTIHNLAFQGRFNPVHMPGTGLDMALFYSGLLEYEGDVNLLKGAIILSDQ
                     INTVSPRYAQEIQTLEYGAGLHEILASRHDDLHGILNGVDYSVWHPSRDPLIAAPYTQ
                     DNPEGKKLCKQALQDTFLLPRSNVPLFGIVSRLHWQKGIDLIVDALEQLVQQDLQIAI
                     LGSGDGALEARINDAVRRHPDKIAVFLGFDASRAHAIQAGSDFLLMPSRYEPCGLSQL
                     YALAYGTIPIVRRTGGLADTVNPWRPGMPDTGEATGISFVPLTPQALYRSVKKALVLY
                     QDGASLRQMRQRAMAMDYSWERSCKDYISLYRRCLEKKQYS"
     gene            14301..14768
                     /gene="tsaE"
                     /locus_tag="PKN92_08445"
     CDS             14301..14768
                     /gene="tsaE"
                     /locus_tag="PKN92_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNZ48861.1"
                     /translation="MNSFEIITHSPEETEVLGEKLAALLPRGTVVALYGDLATGKTCL
                     TRGLTRFFSKNAQVHSPTFTLVNEYGEDPVLYHLDLYRLQGEEELLSLGYEDIIESGG
                     ICLIEWADRAVSFLPPDRLDIFLEHAGGDTRILRFEAGRVADPLAAKLLTLIS"
BASE COUNT         3780 a         4041 c         3770 g         3410 t
ORIGIN      
        1 cggagcagag aagagaccgc cgcaacatac aactatcagc ccgcacaggg tgaaatgctg
       61 accctggaac gcagcgaaat cctgcctgca accgtgcgcc ccggcgaaaa tgcggatgct
      121 agcattcagt atgcgctgct gggaaccggc gggagcattc aggtcagcga gcagcggagc
      181 ctcctgcaga acggcgccct tgttgctgat gtatcctccc actcattcac ccgtgaagac
      241 ggcacctggg tcagcacaca gcccttccgt gtgccctcca atctgcggcc cggaacctac
      301 actatcgtaa cgacagtgcg taccgcacgt tccgccatca gcggacaggc ctccttcacc
      361 gttctgtgag tcattattga acctctgaaa ttgtccaaga ccgcccgaac aaatccgggc
      421 ggtctttttt atcagtacgc acaataaagt ctgaaagaag aatccttgta ctttccccgg
      481 tcaatgcgcc ataattctgt ggacaacttc gggagaatcc tgttatgaaa aaacgctttt
      541 attttcctgt gctgctgctc acctgtctgg cgctgataca cggatgcgct acctgtaaaa
      601 atgagatcac cggccagacg gcacagtctt ttaagagtgt cgtgcaaaaa gaggtggaaa
      661 ttcactacct gctccacttg cccgagggct acgagaacag tctggagccg gcgccgctgc
      721 tcctcttcct ccatggtgcc ggtgaacggg gcagcgatct cgaaaaggtc gcccgtcacg
      781 gaccgcccgc actgatcgcc cgaggagaat ggaatcagcc cttcattgtc gtatcgccgc
      841 aatgtcccgc cgatgcctgg tggacggatg ggctgcagac cgacacgctg gaagcactgc
      901 tggatcatat cgaaaaaaat taccgggtag acaaaaacag aatctatgtt accgggctga
      961 gcatgggcgg attcggcacc tggaggcttg ccacggagaa cccgcagcgt tttgcagcca
     1021 ttgcgcccat ctgcggcggc ggcgatccct tccacgccaa acgcattaaa gagctgcctg
     1081 tctgggtgtt ccacggcgcc aaagacgatg tggtgccgat tctcagctcc gagataatgg
     1141 tcaacgccct gaaacaggtc ggaaatgatg tgaagttcac ggtgtacccc gatacgggcc
     1201 atgattcctg ggtcgccgcc tacgacaccc ccgagctcta tgagtggctc ctgcagcaga
     1261 aacgcagcgg aaaataagga gagctcacca ggaaaaagag agctttatac aaaaaagaaa
     1321 gatacgaagt taaggagtat ttttaccgcc tgagtgcgac cgtaagacaa aagttttcat
     1381 atgtaaacaa tgcagttttg tgttcgataa taacaatcat tgtaaacagg ggagttacat
     1441 catggaaaac aaagctggta cgacaagaat tgatctcaaa gtcctgtggc aggctttgac
     1501 cgatgaagaa aaagaaagag cgtgtcagga tttttgggaa gatccggatc gagggactga
     1561 tctgatccac tacgtttatg accacctttc gcaaatactt cattttcgtg aaaaatacct
     1621 taaaaaacgc tcacttgaag aaaaaacccg atttcttttc aagtttattc tccgtccctc
     1681 tttagaacct gccttggacg ctgcattgcg aagctggctg cttgctcacc accgcagcat
     1741 gatttgctcc tttttggata gccttgggat tcctcatgaa aatggactga tcaacgacga
     1801 tccttcacct cctgattcac aggctttctc tgcttgcatt caaaaactgc tttccgctta
     1861 tgaacctcgc attgtttgca tctatatcgg ataccttgtt cttaccgggg acgatacacc
     1921 ctgggcgaca ttgccaaaag ccgtcgagga gcttcctctt gattttgctg aacacctgtc
     1981 gcaaaagatt ggcacgcagg aagttgtaga gccgaccgtt gaagaagaag aaaaggaacc
     2041 ggaaagaacc agtagcttta caacacttga tcacctgctt ataaaaacga tggtcgcctc
     2101 cgctctgcaa gttgaccatg cccttcccga agagcatgta gaacatctga tagacgaagt
     2161 agtcgaactg aatgttgaac gaaagcattc tttttttcat cgcggctttt tcaacgctct
     2221 tttcaaccgt gaaatggctt ttgattttcc cggtgccaat gacgaacggc gcagctggta
     2281 cttctgtggt gcttttatgg gatatttacg aggaacggat aaagagaaat gccgaaatct
     2341 gctgataaac cagatatcca ttgtgcagga gcttgttaat aatcgggcat tagaatgcgg
     2401 tgccatgctc ctgccatatg tttatccgat tttattaaaa gaccgaaaat ttgacctgtt
     2461 aaagatttgg ctgcttcagc atattttcgg tttatggcct actgaacgtg agcgattttt
     2521 attacatgtt tatcatgatg cagcccggat gcttcggaga ggagcttccg ctgaagccgg
     2581 attattactg gaaacattaa ataaaattgt ttcaaagtat gaaaaacttg attcggactt
     2641 tgtaaaccaa attgaacctt taattgcacg gaaacaaggg caggcatctc agctagaagg
     2701 aaagttcgca gcagcgcaaa cattattttc taaagcgctt gaaaaactgg ataaagaaaa
     2761 tgcagccaat acgcttgccg acctcggtct cgtatccgga gggttcaggt ctttaaaatc
     2821 aatcctgcca gcaggaaaca tggcagatac aaaaggaata gccaactctc ttgctaaagg
     2881 aaaagatttt tatctgcagg caatcgacca gtatggcact ctggcaacaa acgctcattt
     2941 ttgtatgggt attttatatt ttgtcggcgc tgaacaatgt gaggaaaagt gtgctcatca
     3001 tttgcagata gcattggacg gaatgctgca aaagcataaa atatacaatg agaacggcct
     3061 tgtagactgg acacgctttc tgctaggcat cgccctttta gaagtgatgg atcctgcaaa
     3121 tctgcaaaat gccattgagt ccatacagca taatctcgaa aacaaaattc aatacccgat
     3181 gtggctttgg gaaaaagtac tggagtcgct caaagcattt gatgtacctg acacaataga
     3241 agacgtagcc cacagacttt ttcaaaaaag aaataacgaa gcatttcaaa ctctgtggtc
     3301 cactggattt tatacaatag gtgcagtgca gaagcctttt tttgagtgga tcaaaaagaa
     3361 aaaagaagcg acttttgaaa aatggggcga tcttgaaaca cttttccacg ctgcaagaaa
     3421 acaggaagcg atcgagctct gcgaaactat actcgatttc atggaaattg aggctcaggc
     3481 agaacagcat tgtcgtttgc tattcatcga atttttagaa aagaatcctt tactacagcc
     3541 tatatggaac gaaagtgaca ttaatgcatt gctggtacga ctctatgagc tgaacggcaa
     3601 ttttattgat gcggcaccca aactacgtag cgcttttttt caatgcagag acggcggcga
     3661 agcgcatcag attcaggaag ccaggcaaat tattcagaca ttgcaagatt acggattagg
     3721 ggaagactac tggaaagatc tgcatcaatg cctcagcggt cttgagcaga agcagataca
     3781 gatgcaggaa acagaaaata cgttaagagc gggttccgta atccatgttt tatacattgg
     3841 cggtaatgag atccaaactg catataaaga ctccattcag gaggaaattc gccaaaacta
     3901 tccggggctt aaatgtaaat tcctttttcc gggatggaac acaaactggc aaaaagactt
     3961 tgacgaggca aagccttata tcgcagaggc aaatgttgtt gtcattaata aactaatcag
     4021 aacgcattta ggcagggcgt tgcgaaaaca ttgcggaagc agccacccct ggatttcctg
     4081 ctgcggcaga gggagacact ccatccaaaa ccgtatcatt gaggcagcgc aatgggcttg
     4141 tactctgaat aaatataact gatccaaaac agcgcaaaag aacaaaagta catagataat
     4201 taggagagtg tgcataaact ataattagta attgaactgg agcgttgaac cgtatcatga
     4261 tcaatgaaac tgagatcagg tgtttccggc tggcgcgaat gtccccgctg aatcttggat
     4321 tgacactcgc tcttctcgcc ctgccggttg cgtttctcat cgcgtcgttc cttggtgcaa
     4381 ggatgctcac agcacccggc ttgctcatga tggtcttgta cgcctgggtc tggctgcgct
     4441 tacggcccaa gcagttcatc gtgcaccagg acagacttga catcctctgg ccgctgaagc
     4501 gcgtccaaat tccccgcact gacatttcga gcgtacggct gatctcccgg gagaatctga
     4561 aaaatgagat atcccggggt gtgcggatcg gtgccggagg gctctggggc ggcttcggct
     4621 ggctctggac caggcgccgg ggtgtcattc aaatgtatat ctcacgcacc gataacttcg
     4681 tatggatcga gtgtgccggc gcgcgaccgt ggcttatcac cccggaacag cccgaagcct
     4741 tcgttcgggc gttgtcacgg tagcccgggc cgggcataag catacaagtt accggctcaa
     4801 tagtcggagg tccagccacg cctctccgcg gatgttgccg caaccagaac accaccaaag
     4861 caaatgtaat gtgccttagt tgtttacttc ccagccttta tcgtcatgtc ctcaccgggc
     4921 gcatttttct tttaaggaac cgtgttcagt tttccgagta atacgaatcg gaggcttttc
     4981 cgcggaaaac acggtagatc accaccgtat aggcaagaac cacaggcata cccacaagcg
     5041 caatgatcag catcgcctgc agggtgcggg gcgtggagga ggcgttatag gctgtgaggc
     5101 tccaggctgg atccaggctg gataatgcca tgttaggata cagcccgaca gccaccgaag
     5161 caatataagc aagaattacg agagcgctcc cgataaaaaa cagaccgctc ttctgtcctg
     5221 atgcgctgcg gattgaaaga acaaatccac ccgccatcac aagaatagca agccagtaaa
     5281 agagtccgcc ggaaaagaga tgcttcgcct gcagacccag catgaccagc agcagaacaa
     5341 aaaccaccgc cgaagcaaca gccatttttt tgccgtggcc gagcatgcgc tgctgcagcg
     5401 caccatccgt tttcatactc atataagcgg cgccgtgcat ggtaaaaaaa gcgatcccca
     5461 caacaccgcc gatcagcccg gtcgggttca acagcccgaa aaagccgccc gtataatcgc
     5521 cctcggcaga aatgggcagt ccgcgcataa tattggcaaa agcaacaccc agcaacagcg
     5581 ccacaataag actgctcgca ccgaaaacaa taccccatat tttctttgcc gccggcgaat
     5641 gcacatgcgc actgaattcc atggacacag cccgcaggat cagcgcataa agcaccagca
     5701 tgagcgccag ataaaatccg ctgaaaacag cggcgtagac tttcggaaaa gcggcgaaaa
     5761 gagcaccgcc tccggcaagc agccacactt cgttgccatc ccacacaggc gcaatggcac
     5821 tcatacaggt actgcgctct ttttcgtccc tgctgaacag gctcagaata ccggcaccaa
     5881 gatcaaagcc gtccagcacg gcatacccgc cgaaaagaac acctatcaga atataccagc
     5941 ttgtattgag atccatcgtc agtctccttc tttcgccggg aacagagcgg cttctttctg
     6001 taccatgcgc gcaagaaacc agagatacgc aacaagaagc agcgtataga gtgcggtaaa
     6061 cataatcagt gaaaagagaa tatgcgctcc gggaacagtt ctggaaatag cgtcggaggt
     6121 acggagcacg ccataaacaa tccagggctg ccgtcccacc tcggcggcaa tccagcccag
     6181 ctgattggtg aaaacaggca gcggcgccgc cagcacaaga caataaagga accagcgctg
     6241 ttcaaagagc ttccggcgcc agagctgcag cacgccccag cctgtcagcg caataaagaa
     6301 agtgcccagc cccaccataa gatggaaagc ggtgaagggc agaaagagct gaggcagatc
     6361 ttccggcgga aaatcgttcc gccccggcac atgcgtgtcg agacttccgc caacgagcag
     6421 actcagcagc ccgggcgcac gaacttcccc gataatttta tcgcccacag gaatgccgaa
     6481 gagaagaaga ggcgcgcggg tctgtccttc catgagtcct tcaaaacttg ccagcttggc
     6541 aggctggtgc tccgccacct gcacggcatg ccagtgaccg aaaggaaaga gctccgctgc
     6601 cgcggcaatg aaacccacaa tcagcgccag tgtaacggta aggcgtgcct tctcattttt
     6661 actgttctta atgagcagcc aggcggaaac acctgccata aagaaagcac cggtaattac
     6721 agccgccgtg aatgaatgaa aaaaccgggg cagcatggac gggctggtca ccgcttccca
     6781 gaaggaagtc agctccgctc tgccgttctg cagcacatag cccgacggtg tctgctgcca
     6841 ggaattggcg acgaggatcc agaaagcgga caaggtcgaa ccgagggcaa ccatgaaagc
     6901 ggaaaagcat tgcgctgctg cggatatccg gcctctgccg aagaggtaca agccgaggaa
     6961 agcggattcg agaaagaagg ccaatacagc ctccgccgcc agcggtgcac cgaaaatatc
     7021 accgacaaat ttcgaatatt ccgcccagtt tgtgccgaac tgaaactcca tggtgatccc
     7081 tgtggcaacg cccagcgtaa acgtcaatgc cagcaggcgg gcaaaaaaag ccgcagcgcg
     7141 ggcataaact tcttttcctg ttttccagga tcctgtctca agaataacga gcagccacgc
     7201 cagcccgatt gtaataggcg gaaaaatgaa gtgaaaacag atggtggcgg caaattgaag
     7261 acgcgccagc agaatcggat ctaattccat agcatttttt tcctcgattg agtcagggag
     7321 acctataagt tttcccgcag ccagtatgcc cacatcagcc tcttacacgc tccagtattg
     7381 aaagacgctt ccttgtccct gacagtacag cagacacgca ttcctcagac aaaaaggctg
     7441 gctcttactt tgtacatact ttacactatt gctcttttgc ttgcaaaacg gaataattcc
     7501 gctgctgcag caccgatatg ctccggtctg aatccttagt caccgctgtt tacctcttac
     7561 taaaattaat ctcttgcgat atttgtgaaa gttatgccat aataaaggcg cattaaacgc
     7621 tgttttcccg gggtttgttc atcagctgtc cggctttttt tttgcgcttg cggaggcttt
     7681 ttttgtgtct gctgaagtag atgccccgtg gttcgccaag tgtgttatac ttcggtaata
     7741 tggaggtttt ctgttagttt tttctttgcg cccttctttg acgcggtctt ttgttttgaa
     7801 ttttcacccc agtaaacagt gttaacagga atctgacgct atgcagcaac cgtcctgcga
     7861 gcaaatcgct gagttcatca acagcaatct ggataccatt gttcaggatt gcagtgctca
     7921 gattacccct gaccagacgg gtaaattaag ggatttagtg gcggactggg tcaggaatct
     7981 gcttaactct ctgcagggca aatcctatcg cgccggagaa tgggcatcgc acttttcctc
     8041 aacagcacgg gagatggggc tgtccagtcc ggaagccttc acgttgctgg acaaattccg
     8101 catttccctg accggagcat gtaagcagtt tcagccacag cacacggaaa cggaaatccg
     8161 catctgcctt ttcgaagcat cggaaatttt taaaaatcat ctggtggaat attatcttca
     8221 tcaggcagac gaagaagcgg cagccggact ccgcaagcag aaagcagcgc tggaggcctc
     8281 ggcaagcccc atggctgtgc tggacgaaca ggggcgcatc gaaattgcca accgcgaaat
     8341 gggccgtctt ttaaagaccg gctccgacgc actgacaggc acggattttt acagttattg
     8401 caccgaggag acagctgcac tcctgcgtgc ctcccagcga aagaaaactt cttcccagca
     8461 gccgttaacc gtagatggca gcctgaaatt tacccgcact gaattaaaca gccgctttaa
     8521 tctgcgcccc gtttttgata ctgccggaca ccgcagtggc actgttgtat gtctgctgcc
     8581 ggaaagcagg cctacagaaa acgtggaagt gggcatacag tatctggaag accatatttt
     8641 aacgctgctc tctttgcccg tccaagtagt agccgccgac cacaccatcc tttacaattc
     8701 agaagaactg cttaaacttt ctcttcccgg atatgacagt tcgcagccaa tctgctgcta
     8761 cctgaagcag catcagcgcg gttccgaaga ctactggttc tgcaagaccc tttttgagca
     8821 ggggcatttt cactgggaag aaatccattt tgaatgcgac ggagaaaccc gctggtttca
     8881 catgctgaac tttccgctca cggacggcac gggcagtatc gtgcgcatgg ttacctgtgt
     8941 ttacgacaca acacggcgcc gccagattca gaagcagatg gaaagccatc tgattcagca
     9001 gcagcgcagc tcactcgtct cccagatcgc tgtcactgta gcgcatcagc tgcgcaaccc
     9061 gctcagcgtc gtattgggct ttgcggaaat gctttccaag ggactgcccc cggatcagta
     9121 ctccgaagca gtgaaccgta tccaccgcaa cagcctccgc tgcaaagata ttgtggaaaa
     9181 ccttctggac ttcggtaaag gcacgccgct ggaacgacgc cccgtcgatt ttgaaaatct
     9241 cattcagaaa acagtgcgca tacgcctgac cccggcgcag gatcgcctga tcgagtggcg
     9301 tttttccgga aaaccgacgc cggtggagtg cttgcccgaa caactggcgc aggtagtaca
     9361 cagtctgctg cagaacgctt tgaaagtggc acatacccgt atcctttttt ctgtagaagt
     9421 caaaggctat tttgtgcggc tccgcatcgt cgatgacggg ccgggcatcc ccatcagcga
     9481 cagggaacgt gtttttgagc ctttctatac atcccaccgg aaagaaggcg cggtgggact
     9541 cgggctcagt ctcgcccgcg cccttgccaa tgattacggc ggatcactca ctatctcaac
     9601 acccggtgct gaagagccac agggcgcctg ttttattttt cagctgcctt tgctgtcgga
     9661 accgaaccac ccggaagaaa aacaggcacc tgaaacgaat atcagcggga acgagcatca
     9721 gattctaatc gttgacgaca atgccgacct gcgcgatctg ctgactacga cgctgtacaa
     9781 ccgaggtttc caggtaacca ctgtcacctc cggtatggcg ggactagaaa aactgaagca
     9841 gcagcactac gacgcaatca ttctggattt tctgcttgaa ggatcgctca ccggccgaca
     9901 gatctatgaa gaaattcagg caagccatcc tgagtttgca gacaaagtac ttttcatcac
     9961 aggggatatg ctgacctatc agacacgcct ctacctggaa agcacacatc ggcccgtgct
    10021 ggaaaaacca ttcctgatga cagattttct ttctgcaatc gccaaaataa tccaagactg
    10081 accgtacctc aggaacgggc tcatcataac ctgacagaag gaggctgcgc ctgtgtcttc
    10141 ccccatgcga atactgtttc tggctttaac ttctgctgtg gcttcttttt ccctccttgc
    10201 cgaagaacag cgaatcccgg cggaaaaagt acttgccctg accccttccg aaacgaaccc
    10261 gcgtaattcc gaaggcgatt ttatcgccct tgccgacggc acacttcttt ttatttacac
    10321 ccgattttca agcggggcag atgatttcga tgccgcctgt ctggcatcac gccgctccga
    10381 agacggcggc gccacctgga gcactgagga tgaaattgtt ctcgaaaacg aagggcagcg
    10441 caatgtaatg tctgtatcac tgcttcgtct tgagagcggc gaaattgccc tgttttatct
    10501 ccgcaagaac ggtgcgcagg actgtcgccc ctgcatgcgc gtgtcccggg acgaaggcag
    10561 aagctggagc gatgccgtcg aatgcatccc tgagcccgtc ggttattatg tggtgaacaa
    10621 cagccgggtc attcagctgc gttccggacg gctggtcata cctgcggcaa tccatgcgct
    10681 tcatggtgca ccgtttaaag agcgaagtac tgccgtctgc tttctttccg atgatgcagg
    10741 aaaaaactgg taccgctccg acactgaact gactgcgccg gaacaggcac gtgccggatt
    10801 tcaggaaccc ggcatcgtgg agctgcccga cggtaccctg ctgatgcatt tgcgaaacga
    10861 tctgggggtc ttttatgaat cccgttcggg cgacggcgga acaacctggt ccgatgctgt
    10921 acctacaacg ctcaaaacac ctgtttcccc gggcacctgc gtgttgatcc ccggctcaga
    10981 gcggctcctt cttctctgga acgatcactc acaaatcgaa gaggcgctcg ccggcaagcg
    11041 aacgcctctt acattggctg tatcagatga cggtggcaga cattggcaca gcacccgcct
    11101 gattgaagac aatccggcag ggtggtactg ctatacggcc gcccggttca caaaaaccca
    11161 cttgctgctc ggatactgcg ccggagacac acgatccatg ccggggcttt ccctgacaca
    11221 gatcacccgt atcgccctga ctgatctcgc cgctctctaa tcggttccgg cgttacgcca
    11281 gactttttcg gtttgcccac actttttcaa acgcatcgcc gtactgctcc accagctccg
    11341 gctggtcgga agtccagtaa ggaagctgga tcgcctgacg gttcgcctga tcgcacccgg
    11401 gcaccgcgtc gggcagcaca ggcatctgcc gccagtactt ggattcgccg aatattggat
    11461 aggtgtggag cagcgtccag gtgaaggcgg tcacgtcaac accctccgcc tgcagtgcgg
    11521 caatggcatt ttctctggac atgcccgctt tggcctcgtc aataaagagc atattcttgg
    11581 aatagaaaac acgctcgatt ccctcacgga catattgcgt tgtcagaccg ggaagctggg
    11641 tgatgcggtc gttcagtttc ttcatctgtg ccttgcctgc cgcattgcgc tcttccagtt
    11701 tttccagctg gaatttgccc aaaatggcgg caaccggatg aatacgcagc ttcgagccga
    11761 aagcggtgcc ctgatatttg cggtaggggc tgtcatcggg gaacgtgttg ggcagctcat
    11821 agttgccgta ggttacggca cgctcaaaat cgccgcggtc cttgtacatc gccatgccgc
    11881 cttcaatggc gggcagcggc ttggaaccct ggaggctgaa gcccgccatg cgtccccagt
    11941 tgccgatccg cacaccattc actttggctc cgtgcgcatg gctggcatct tccagcacaa
    12001 tcagatcatg ctctttggca aaagcggtaa tgtcatccat ttcgcagggc agcccgaacc
    12061 agtgcacagc gagaatggcg cgcgtttttc cggtgagccg tttcgcacag tcctccagat
    12121 ccagattcat ggtatagggg ttcacgtcca caaatacagg aaccagatca aagaagcgca
    12181 tgggcaccac cgggaaccag gtgctgtaat tcgccacaag tacctcgctg cccgccggaa
    12241 ggtccaatgc gaaaagcatg gaggtcagcg cactggtgcc gttaaaatgc gcttttgtga
    12301 aggggcagtc atggaacact ttccacgcct cttcaaattc ggcaatcggt ccgtagccgg
    12361 gatccttcag gagagcagtc accgcttcta tttcttcgga tccataaatc ggccagcggg
    12421 tggatccttc agcggaagcg gtaaccgcct tggcaccgcc tttcagcgca agcgcttccg
    12481 ctgcctgagc atttccagag gtcattgcgc ctcccagata aagaccggca cctaatgcgc
    12541 ccgttgtctt caaaaagtcc cggcgcgtcg cctccgtatg cgtgtgtttc ttttccacga
    12601 ctcagatctc ctgtttttcg ttgtaaaaaa tgccgaatgc acctttcata gagtcttttc
    12661 attctacccg cgacaggcaa caggtgtcaa taggctggca gcacggatcc atgacgctgc
    12721 gttcggacgc caccgaagaa cgcgtgcagt ctctccgatt tctatcgcat gacctctctc
    12781 gttctgatat gctacactac ttatgataac ccacaggaaa gaatgattgt gaacaaaagc
    12841 ctgcgcatcc tcttcgtcgc ttccgagctg acaccgctta tgagcaccgg cggtcttgcc
    12901 gaagtggccg cggctctccc gcccgcatta cagcatgcag ggcatgatgt acgcattgcg
    12961 ctgccctgtt accggcagat tcctcaggag gcgcgaggcg tcgttatcac cgcctgcgaa
    13021 gcctctgcag acggatatgc ccgccggggc gcactgcgtc gcactgttgt acccgatact
    13081 tccatccccc tttatctggt ggaacatgag gattttttcg gaagagacgg agcctatggg
    13141 agcggctcct atgaatatac ggataacgga gaacgcttct cctttttttg tcgtgcgctt
    13201 ttggatgcgc tggaaaagat tgactggcag cccgatatta ttcactgcaa tgactggcag
    13261 acggccgccc tccccatttt tctgaaaaca cagctgcagg cacgcccctt ctggcagcgg
    13321 accgcttgcc tgctgaccat tcataatctg gcctttcagg gcaggttcaa tccggttcat
    13381 atgcccggta ccgggcttga tatggcgctt ttttattccg gactgctgga atatgagggc
    13441 gatgtcaatc tgctgaaagg ggctattatc ctctccgacc agatcaacac ggtcagcccg
    13501 cggtatgcac aggaaatcca gacgctggaa tacggtgccg ggctgcacga gattctggca
    13561 agccgccacg atgacctgca cggcattctg aatggcgtcg actacagcgt gtggcatcct
    13621 tcccgggatc ccctcatcgc tgcgccttac acccaggata atccggaagg aaaaaaactt
    13681 tgcaagcagg cgcttcagga taccttcctt ctcccgcgca gtaacgtgcc gctctttggt
    13741 atcgtgagcc ggctccactg gcagaaaggg attgatttaa tcgtcgatgc gctggaacaa
    13801 ctggttcagc aggatttaca gatcgctatt ctgggatcag gagacggagc gctggaagcc
    13861 agaattaacg atgcagtacg gcggcacccc gataaaattg ctgtatttct cgggtttgat
    13921 gcatcgcgcg cccatgccat tcaggcagga agcgactttc tgctcatgcc ctcccgatac
    13981 gagccctgcg ggctgagtca gctgtacgcc ctcgcctacg gcaccattcc catcgttcgc
    14041 cgtacgggcg ggctggcgga taccgtaaat ccctggcggc ccggcatgcc cgataccgga
    14101 gaagccaccg gcatttcttt tgtaccgctg acacctcagg cgctgtatcg cagtgtaaaa
    14161 aaagcgctgg ttctctatca ggacggggca agtttgcggc agatgcgcca aagagccatg
    14221 gctatggatt attcctggga gagatcctgc aaagactata tatccctcta tcgtcggtgt
    14281 ctggaaaaga agcagtactc atgaattctt tcgaaatcat tacgcacagc ccggaagaaa
    14341 ctgaagtgct gggcgaaaaa ctcgccgccc tgctgccccg cggaactgtc gttgctcttt
    14401 atggcgatct tgccaccgga aaaacctgcc tgaccagagg gttaacccgg ttcttttcaa
    14461 agaatgctca ggtgcacagt cccaccttta ccctggtgaa tgaatacggg gaggacccgg
    14521 ttctttatca tctggatttg tatcggctcc agggcgaaga ggagctgctc agtctggggt
    14581 atgaggacat aatcgaatca ggagggatat gccttatcga gtgggcggat cgtgccgtct
    14641 ctttcctgcc tccggacagg ctggacatct ttctggaaca tgccggcggt gatactcgaa
    14701 tactgcgctt cgaagcaggg cgagtggctg accctctggc agcaaaactc ctgacattaa
    14761 tcagttaatc cgtctgcaca ccgatcttgt tttgcagcca gacccctact ctggaataat
    14821 ttacacaatt cttatcgtta cagcagctgt gttgacacaa ccttacagat gacaccgctg
    14881 tctgcagtgc aaacactttc agtcaatgta tgccccgaaa acaaaaagcg ggcctccccc
    14941 tttttatggt tatgtcaaga agtttctgta aattgctttt cctggtgaac accttgttat
    15001 t
//
LOCUS       DAOPWB010000079        14240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4354_length_14240_cov_6.029891, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000079 DAOPWB010000000
VERSION     DAOPWB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 14240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14240
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4354_length_14240_cov_6.029891"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..956)
                     /locus_tag="PKN92_08450"
     CDS             complement(126..956)
                     /locus_tag="PKN92_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48862.1"
                     /translation="MKKIFLYFAILALLPMLNTFGADVTVEVNPLPRDFPLNIESRIS
                     WTFSWTDADASRALLPPVLPEIEWGRLRVLSARIHKNGAQMKAVTVLGITPVQPGNHV
                     LPALTFQFFPLEEKDKGEQSVTELLPAASESVITEALDIHAGGKTPFLFPAGLGGSAL
                     FLACFLLYLRRRAKKRSSSAVAEPTLDERLSSLLHQARRLRLDGSYYECYRLLHSIAL
                     QCNDNGRTVPPALLSRLEKGMNDTGYRGKRPSGTDLEGDFKDVEQVCALSRAHETQGV
                     "
     gene            1448..2671
                     /locus_tag="PKN92_08455"
     CDS             1448..2671
                     /locus_tag="PKN92_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNZ48863.1"
                     /translation="MSHSQNALFRAALAGLLFFLTFTGITLSSHAGEGVFLLGNDALQ
                     LGRASSGVASPRSAYWSYMNPASMVDLERRLDVNWYSVLATSRMTARGLISNPFDGTQ
                     ESENLFNIVSTGFILPLKTGTLGGGLFTPSGAGVDYPHSRTIMGRLLGNTDRRLSYQH
                     IRGVLSYAYRFDNGWSVGLGLHLSLNRFRTDHITLGLSGAEYNNKWDSALGAGLGFGV
                     YRAWDKLSVGLNYSSRHWTEAMSKYADLLSAPLDTPRTLQVGFAYRFTPKFEVTADYK
                     WLHWRRIRTYGSPMFRSGGFGWTNQNGIKLGAEWKVHPKWTLAAGYAYSNTPVQKDAV
                     FLSALVPVVVEHHATLGVSHAINEKNEIHLSGVYAFRNREREAGCGDLLSILGRNTTI
                     ESKAVSAALGYTYKW"
     gene            2869..4617
                     /locus_tag="PKN92_08460"
     CDS             2869..4617
                     /locus_tag="PKN92_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HNZ48864.1"
                     /translation="MSSSSSKPPVSFSVNTTIERDRALLNTARAKGRLPFLGVLIRLS
                     GPGWLQSAITLGGGTLSNSLYLAVLTGFTFLWLQPVAMAVGIIMLSAISYVTLSTGER
                     PLRMINRHINPVLGWSWLFASMVANLVWSMPQFGLALASLTKLLFPQVLGQAGLFGSH
                     GRLAAALCILVLNILFLSLYSSGGKGMKIFETIMKCMVGLTVFCFFGVVFVITRDGLA
                     DWREYMGGFIPRVNMMFEPGPKLAPYLSEVPEQFRAFWTGKILSDQRDFIIAAFATAV
                     GINMTIMFPYSMLRKGWDRDFRGLAIFDLSTGLFIPFMLATSCVVIAASTQLHAKVPA
                     GLISPVDASGKKITVDASLQKSYDGLLTERIQWERAQAGGASPENEALADRISTLDIS
                     EKTLAAMLARRDAPQLAASLEPLTGSLIARYIFGLGILGMGISAATMLMVINSLCFCE
                     LLNRPARGWPQFVGGAMTSISLFVMLSWDGALMAVATPTSVFCMTLLPLAYLTFFLLM
                     NQKKVLKDAMPRGGKRILWNSLMSLSLFCAFFGSLWGIYGKIGGRWTAVVMTVFTLLV
                     AAGHIYRKTISPAAEE"
     gene            4667..5740
                     /gene="amrS"
                     /locus_tag="PKN92_08465"
     CDS             4667..5740
                     /gene="amrS"
                     /locus_tag="PKN92_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNZ48865.1"
                     /translation="MGQSSQPSADYQALLDAYTAPADRLLVEKLPDKAVRCLACAHRC
                     TLSPGQRGICRLRFNQDGELRVPYGYLAGLAVDPVEKKPFFHVLPGRSSLSFGMLGCN
                     FRCDFCQNWQSSQALRDHDACTGLTPNTPEQVLALAQKNGLPALISTYNEPLITSDWA
                     LALFEEAHAAGIKCGFVSNGFATPELLSRLLPFMDMFKVDLKCFTAEGYRKLGGRLAP
                     VLDTITRLAASDVWLEVVTLIVPGFNDDEKELSAIAAFLASLSPDIPWHITGFRPQYR
                     YQDADATSADVLTRAWNIGRDEGLHFVYTGNTGLCRQAREHTLCPGCGILLIERQGFF
                     VVRNRLHQGRCPDCSTSIPGLWH"
     gene            complement(5749..6858)
                     /locus_tag="PKN92_08470"
     CDS             complement(5749..6858)
                     /locus_tag="PKN92_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HNZ48866.1"
                     /translation="MTDDGAQGGRFFARTGFWLTTALLWLLCGVWYFLPRQSGQVELW
                     YSRGLFKSLAFLIVPLTDSLPFSLAITLLVAFPLVLAGVFVAEVISALRQKEKVTIRV
                     LSRGIKILLFLLPFLWCWFLLFWGIGYGRDPMETRLHMDNEAVAESELLQIADTLYGV
                     LTRDLPRDAEERNISRAVRSISYAMQRVVLRWEGRSAAVPRRVKATPPGLLLLNGTSG
                     ICSPYTLEAHVDGALPDTFFVAVAAHELGHIAGVCDEGETNLIGYAAGLQAENSYARY
                     CVALDLYVDVARQLSPELRQAAMARLPQSALDDIRKAGEASRRYRIEFFHKWSWRMYN
                     HYLKSQGVKDGVRSYGRGVQLVVKAWRAGMLPLAG"
     gene            complement(6871..9441)
                     /locus_tag="PKN92_08475"
     CDS             complement(6871..9441)
                     /locus_tag="PKN92_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNZ48867.1"
                     /translation="MTDIPTSAEVQPGQEQAPVFTSLLQAVAAKNIYASLVLIEKGAA
                     DNETDETGKNALLLAAGSNMPAVVDLLIEKGDNLLAKDALGNTALHLAARANDVYTTA
                     MLLDKGIPIESLNERIYAPLLEAADAGSLDTCRFLVEKGESPLTSYDEETYFSAFHAA
                     AKNHRWDVTALFREMGYRPDVFQDAALGDLPRLQKYADEDPAQFSLVSRTGMTPLIAS
                     MASNSPEAVEIILKTGIRQTSNLEGETPAVLAVKAGRQDYLALFFKYGASPDGRDGGF
                     YGWTLLAHATMTEDLEMMDFLLRNGAHIGYLSAKGEAPIHVAAARNLESALRFLLERG
                     AIVDTRSGAAQTPLHLAAEKNNTVFAELLLANGAKIQATDKRRWTPLHIAADAGNEAM
                     IRLLLANNADVNAQDKQGRTPLNLATAKGNHAIAALLLESGTDPDLSDQKEQTPLHRA
                     AALGDMQLSKTLLDSNAIVDSTDVTGKTPLFVALEEDHYELARMLAEAGASLGIKDNE
                     GATLLFPAASNENDSAVRWLVGQQLDVNAVDEKGTAPLHVAARFGCEDSIKFLVAQGA
                     SVNAADKDGCTPLHLAAARGHMMNINALLDKGADMRARDHQGWLPLHYSAAKGHWGPL
                     QLFILRGIPVNEPANDGNTALHLIVQGGFHRTASLLLTRGADFTARNAAGETPMDLIK
                     KAREDYEARNDLSSLEKATRVALAQTQVFFQAVLLDELSNAVERKDKAFLQSFLDSYP
                     ELVNGSLFGRSVLYRAVTGGDVPVVTLLLERGADPNVPAVTKNKSTPLHLAAERSNKE
                     LIELLLKAGADVDQPDATGVTPLAVARRIGRTDIIDRMSEFSGKQGKTLQEGAAAEQK
                     "
     gene            9900..11036
                     /locus_tag="PKN92_08480"
     CDS             9900..11036
                     /locus_tag="PKN92_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48868.1"
                     /translation="MKHVRLLHAGALRLNNTFEALGVSPETGDALRAAQKDVLEKLAQ
                     QAQSLPADMIILSGGLFATLPVRADLLLRVLECLEQVAPTPVYIFPGAHEGLHDLSPW
                     DFALVPDNVTVFSGTAWSSVDHPHLPVTIHGRTDAATPLDFRNLHFPEDGRIHIAGID
                     CGNTSLDELQEVPSRLAYLALGTCQITADLKEKAGVCGCAAAAPQALSFDECGEKHFF
                     KVNITLDDHASIHTTVETLPSSSLLFEKRSLDIADLPSPDENAAQLLALPEQHLKRVL
                     LKLVITGTKNGRMIDHLHGFIKHLRPKLQHLEVEDMSRLNPDSLSAAGTEILTRDIAA
                     QILQRLRDCTDYEEKQRLEMSLRLLFKAVYDTHAGLNAEGLSEP"
     gene            11033..14053
                     /locus_tag="PKN92_08485"
     CDS             11033..14053
                     /locus_tag="PKN92_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48869.1"
                     /translation="MILTTLSYLHPETRESVVRALGTERCLLYCPASETARFLSTEIH
                     QQQPAPLETANEASQVFSLPGYSSVRLDYRKRITAGLQDSLFAEVSDSNPAPDDPDSD
                     AFFRGQQDLFHLSLGDALQAEHEKEALLVTQLAPLIESGSSAKGLTEAMRILDELAVS
                     LGRGNRPGSYLTSLKEKIHKLAEEKKQTVSMPSELFAVAKTLKENRCRLKELGSRRAA
                     LQKQIDLLTARNVKNLEKEIADLESARDALGTTPLLSSEQMAELNRMTTLVKTVQCQL
                     EKTGDELRLVQEKLELLPGESSHKYKAPATYPEALERSIRQTSNKIAENEVRLDEIQQ
                     QMDREDRFITQAQAELSSLPDFSKLPPDPADFLNIVLQSCKNAIEEQKNEAERCASLE
                     KELDRLQRAWEIHGPIFEQAGDFTSIMVQNEQKRKAARERYPLIAEEVDKKQSIRDEQ
                     QDFQQSQQWLAIGCFLLMCGAAGAYFFLEKTPILLVVAALLICVVYFFLHAFFADRQV
                     RNLNQAIAEDHAELDLLQETINHRDTYIEDLMQQLGCQSPRELSTLYENHREITQQII
                     DTSKEVEQARQLCLECEERVPALFETLCAHLALVGKVPESTSIMDSYVADAIANYRRC
                     KDVKLQLANRQNCRQSLFARKTFFDNEQHAQRDKFNQQMEQFRTLMRENGFAQEVVYT
                     ELDDLCANYRSFLDDALTSMDSSELLARQAQKLQERERNDREQLARYEEQRQTLLKAL
                     GFSTLEEAAEAGDQGAKFKAIQAEIDLLKAKLNTYRQGPIRVQGELLLPEGLAGDEEE
                     GALRQLNASLNQVEKECAAVRQRWQDAWLRYSRLTTEQRPSGVIAEELLWTEKNLQQV
                     QKIISAGALVVSLIQESLLTDRPERCALIAARATELLQTAGLSSARVQIALNAEGKAS
                     ITAHADTATEMPSPLTLYMAVKIAAALVLAGEAGNRLFIVDAALQQEELPTGTDEFFT
                     LLDALPRHWQTLVVTANRTLVEAGAARGWQTQNL"
     gene            complement(14059..14136)
                     /locus_tag="PKN92_08490"
     tRNA            complement(14059..14136)
                     /locus_tag="PKN92_08490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14099..14101),aa:Pro,seq:tgg)
BASE COUNT         3370 a         3860 c         3639 g         3371 t
ORIGIN      
        1 ccctctcccg ggcacatgaa acacaaggag tctgataagc catgtcggtg aaagtagatg
       61 cagtccgccg cagtgtggaa gaacacgctg cggcggactg catctacatt caccgacatg
      121 gcttatcaga ctccttgtgt ttcatgtgcc cgggagaggg cacacacttg ttcgacatcc
      181 ttaaagtcgc cctcaagatc ggtcccggag gggcgcttac cacggtatcc cgtgtcattc
      241 atcccttttt ccagacggga aagaagggca ggcggtacag tacgaccgtt gtcattgcat
      301 tgcagtgcga tgctgtgcag caaacggtag cattcgtagt aactgccgtc caggcgcagg
      361 cgtcgtgcct gatgcaacag gctgctcagc ctttcatcca atgtcggttc agccacggca
      421 gaactgctcc gcttttttgc tctgcgccgc aaatacagga gaaaacaggc gagaaacagc
      481 gctgaaccgc ccagtcccgc agggaaaaga aagggtgtct ttccccccgc atgaatatcc
      541 agcgcttcag ttatcacgct ttcagatgca gcaggcagca actcagttac gctctgttct
      601 cccttgtctt tttcttcaag gggaaagaat tgaaaagtca aggcaggcaa cacatgattg
      661 cccggctgca caggcgttat accaagaaca gtcacagctt tcatctgagc gccatttttg
      721 tggatcctgg ctgacagtac tctcagtctg ccccattcaa tttccggaag taccgggggc
      781 agaagcgcgc gagatgcatc cgcatccgtc caggaaaaag tccagctgat ccggctttca
      841 atattcaaag gaaaatcacg gggcaaaggg ttcacttcaa cggttacgtc tgcaccgaag
      901 gtattcagca ttggcagaag cgccaggata gcaaaatata ggaagatttt tttcatacac
      961 ttctaggagc aactaacgtg ccataatttt tatcccgtat cttgctgttt tataagcact
     1021 taggtttact ttaaaggaaa gcgtttcctc aaatcaggtt taagcagtgt tgctgcactg
     1081 tttcggggta tttcggactt tttttgcgtg catcctgccc tggctgttct ctatcaagtg
     1141 gatgtctgaa tctttttttt ctttttatca atctaacata agtcgtaacc atgtaattct
     1201 aaaacagcaa tagaatgtga agggattgta tctcattgca caatactaat aatattgaca
     1261 ttcattatag aacttccgaa ttccttttga atccactaaa agcactatgt tataaatatt
     1321 cagattttgc ttaaaatcgg acagtcttcc catccggtta aattatgcca gagccgagca
     1381 ttatagcagc gggcttcatc ctattattat ttaatctttt tatactttta atccaaggag
     1441 cgctctcatg agccattctc aaaatgccct tttccgggca gcccttgcgg gcttactctt
     1501 tttcctcact tttacaggga ttacgcttag ctcccatgcg ggagaaggtg tttttcttct
     1561 gggcaacgat gctttgcagc tggggcgagc ttccagtggc gtggcaagcc cgcgcagtgc
     1621 gtactggagt tatatgaacc cggcttccat ggttgatctg gaacgccggc tggatgtgaa
     1681 ctggtacagc gtgcttgcca cctcccgcat gaccgcccgc ggcttaatca gcaacccttt
     1741 cgacggcacg caagaatctg agaacctttt caatattgta tcgacgggct ttatcctccc
     1801 tttgaaaacg ggcacgctgg gcggtggtct ttttacccca agcggcgccg gtgtggatta
     1861 tcctcattcc agaaccatca tgggccggct tttgggcaat accgatcgac ggctgtcgta
     1921 ccagcacatt cgcggcgtgc tcagttatgc ctatcgtttt gataatgggt ggtctgtggg
     1981 tctggggctt catctttcat tgaaccgctt caggacagat cacatcactc tgggactttc
     2041 cggcgccgag tacaacaata aatgggacag cgctttaggt gcagggctgg gcttcggtgt
     2101 ctatcgcgcc tgggacaagc tttcagtagg attaaactac agttcgcgtc actggacgga
     2161 ggcaatgagc aaatatgctg atttgctgag tgctcctctg gatactcccc gaacgcttca
     2221 agtcggtttt gcatatcgct tcacccctaa atttgaagtg acggctgatt acaaatggct
     2281 gcattggcga cgcatccgca cgtacggctc ccccatgttc cgctccggcg ggttcggctg
     2341 gacaaatcaa aacggcataa aactcggcgc agaatggaaa gtgcatccga aatggacact
     2401 tgccgccggc tacgcctaca gcaacacacc tgtacagaag gatgctgttt tcctttcagc
     2461 actcgttccg gtcgtcgtag agcaccacgc cactttgggt gtatctcatg ccattaatga
     2521 gaagaatgag attcatttat cgggggtcta tgcgttcagg aatcgggaac gggaagcagg
     2581 atgcggcgat ttgctttcca ttttgggacg caacacgact atagaatcaa aagcggtgtc
     2641 ggcagcattg gggtacacct ataagtggta accgggtgcg ggatctgcac ccgcacttct
     2701 tcaatttacc tttatgcttc ttccggaagt tcgggcagcc tgctttattt ccgaattatt
     2761 tgtgcacgcc ttgtttctgg aactcttcca aatcaatgtg ctattctcaa accggtcttt
     2821 ttcaggtctg caggatctgt tccgattcag catgaggaag gaaaaaagat gtcatcctct
     2881 tccagtaaac cgcctgtcag cttttcagta aacaccacca ttgagcgtga ccgggcgctt
     2941 ctcaacacgg cgcgtgccaa aggcagactt ccttttctcg gcgttctaat acgcctctcc
     3001 ggccccggct ggctgcaaag cgccatcaca ctgggcggtg gcaccttatc gaacagcctg
     3061 tatctggcag tgctcaccgg ctttactttt ctctggcttc agccggtagc aatggctgta
     3121 gggatcatca tgctcagcgc catctcctat gtcaccctgt cgaccggaga gcgaccgctg
     3181 cggatgatta accgccacat taatcctgta ctgggctgga gctggctttt tgcttccatg
     3241 gtcgccaatc tggtgtggag catgcctcag ttcggactgg cacttgcctc tttgaccaaa
     3301 ctcctttttc cgcaagtcct gggccaggca ggtctcttcg gctctcacgg ccgactggcc
     3361 gcagccctct gcattctggt cctgaacatc ctttttttaa gcctttacag ctccggcggc
     3421 aaaggcatga aaatcttcga gacaatcatg aaatgcatgg tgggtcttac ggttttctgt
     3481 tttttcggtg tggtctttgt cattacccgg gacggacttg ccgactggcg tgaatatatg
     3541 ggcgggttta ttccccgggt aaatatgatg tttgagcccg gtcctaaact ggccccctac
     3601 ctgtctgaag ttccggaaca gttccgcgct ttctggacgg gaaagattct gagcgaccag
     3661 cgcgatttca tcatcgccgc ctttgccaca gccgtcggca tcaacatgac gatcatgttc
     3721 ccctattcca tgctgcgcaa agggtgggat cgcgatttcc gggggcttgc catatttgat
     3781 ttgtccacgg ggcttttcat tccttttatg ctcgccacat cctgtgtggt aattgcggct
     3841 tccacgcagc tgcacgccaa agtaccggcg ggacttatct cgcctgtgga tgcgtccggc
     3901 aagaaaatca ctgtagacgc aagtcttcaa aaaagctatg acgggctgct gactgagcga
     3961 attcaatggg agcgtgcgca ggcaggcggt gcgtcgccgg aaaacgaggc attggcagat
     4021 cggatttcta cgctggatat ctctgaaaag actcttgccg ccatgcttgc gcgacgggat
     4081 gccccgcagc ttgcggcatc gctagagcct ctgaccggat cgcttatcgc ccggtatatt
     4141 tttggtctgg gtattctggg aatgggcatt tcagcggcaa cgatgctgat ggtaatcaac
     4201 agcctctgct tctgcgaact tctgaaccgt cctgccagag gatggccgca gtttgtgggt
     4261 ggggccatga cttctatttc cctctttgtc atgctctcgt gggatggagc gctcatggcg
     4321 gttgcaaccc cgacttctgt cttctgtatg accttgcttc ctcttgccta tctgactttc
     4381 ttcctgctga tgaatcagaa aaaggtgctg aaagatgcca tgccccgggg cggaaaaaga
     4441 atcctgtgga attcgctcat gtccctttcc ctcttttgcg cctttttcgg aagcctctgg
     4501 ggcatttacg gcaagatcgg cggtcgctgg actgctgtag tcatgactgt ttttactttg
     4561 cttgttgcgg caggccacat ttatcgcaaa acgatcagcc ccgccgcaga ggaataatga
     4621 cattgggttt gtgcgtgttg gatgcgcctt taacctggag catgcaatgg gtcaaagttc
     4681 acaaccatcg gcggattatc aggccctgct ggacgcttac acagcaccgg cggacaggct
     4741 gctggtcgag aaactacccg acaaggcagt aagatgcctt gcctgtgccc accgctgcac
     4801 actgtcaccc gggcagcgcg gcatctgccg tcttcgcttt aaccaggacg gagaactgcg
     4861 ggttccttat ggatatctcg ccgggcttgc cgttgatcct gtcgaaaaaa agcctttttt
     4921 ccatgtgctt cccggacgct cttctctctc tttcggtatg ctgggctgca attttcgctg
     4981 cgatttctgc cagaactggc agagcagtca ggcgctccgt gatcacgatg cctgcacagg
     5041 tttgacgccg aataccccgg agcaagttct ggcacttgca cagaaaaacg ggcttcccgc
     5101 tctcataagt acctataacg aaccgctgat tacgagcgac tgggcactcg ccctttttga
     5161 agaggcacac gctgccggga ttaaatgcgg ttttgtgagc aatggctttg ccacgccgga
     5221 gctcctctcc cgactgcttc cttttatgga tatgttcaag gtggatctga agtgctttac
     5281 cgctgaaggc taccgcaaac tgggcggacg gcttgcacct gtgctggaca cgataacccg
     5341 attggcggca tcggatgttt ggctggaagt agtcacttta atcgtacccg gatttaacga
     5401 cgatgaaaaa gaactctccg ccatagcggc atttctcgcc tcactcagcc cggacattcc
     5461 ctggcatatc accggcttcc gcccgcaata cagatatcag gatgcagatg ccacctccgc
     5521 tgatgtactg acccgggcgt ggaatatcgg gcgtgatgaa gggctgcact ttgtttacac
     5581 cggaaatacc gggttgtgca ggcaggcacg tgaacatacc ctctgccccg gctgtggcat
     5641 actgctgatc gaaaggcagg gattttttgt ggtgcgcaac agacttcatc agggcagatg
     5701 tccggactgc agcacctcca tccccgggct gtggcactga tttgtcagtc agcccgcaag
     5761 aggaagcata ccggcacgcc acgcttttac cacgagttgc accccgcgac catagctgcg
     5821 caccccgtct ttcactccct gcgacttcag gtaatggttg tacatgcgcc agctccactt
     5881 gtgaaaaaat tcgatacgat accggcggct cgcctctccg gctttccgga tatcatccag
     5941 agcgctttga ggaaggcgtg ccatagccgc ctggcgcaat tcagggctta actggcgggc
     6001 gacatcaacg taaagatcca gcgcaacgca gtaccgcgca taactgtttt ccgcctgaag
     6061 accggcagca tagcctatca gattggtttc gccttcatcg catactccgg cgatatgtcc
     6121 cagttcgtgc gcagcgacag ccacaaaaaa ggtgtcgggc agtgcgccat ccacatgcgc
     6181 ctccaatgta taaggagaac agatgcctga agttccgttt aacagcagaa gcccgggcgg
     6241 ggtcgccttg acacggcggg gtacagcggc acttcgtcct tcccagcgca gaacaacccg
     6301 ctgcatggca tacgaaatgg atcgaactgc acggctaata ttacgttctt ccgcatcccg
     6361 ggggagatcc cgggtaagaa caccgtaaag cgtgtcggca atctgcaaca gttcgctctc
     6421 cgctacagct tcattgtcca tatgcagccg ggtttccatc gggtccctgc catatccgat
     6481 gccccagaaa agcagaaacc agcaccagag aaaaggaagg aggaaaagca gaatttttat
     6541 tccgcgggaa aggacgcgta ttgtcacttt ttctttttgg cggagagcgg aaatcacttc
     6601 agcgacaaaa acacccgcca gcaccagagg gaaggcaaca agcaatgtta ttgccagaga
     6661 aaagggaagg ctgtctgtta aaggcacaat cagaaaagcc aggcttttga aaagcccccg
     6721 ggaataccac agttccacct gcccggactg ccggggaaga aaataccaga cgccgcataa
     6781 aagccagagc aaagctgttg tcagccaaaa gcccgtccgg gcaaaaaaac gaccaccttg
     6841 cgccccgtca tctgtcataa caaacccacc ttatttctgt tctgcagcgg cgccctcctg
     6901 cagcgttttt ccctgtttac cggagaattc gctcatgcgg tctattatat cagtacgacc
     6961 aatacgccgg gcaaccgcaa gaggtgtcac gccggtggca tcgggctgat cgacatcagc
     7021 ccccgctttc agcagcagct caatcaattc tttgttactg cgctcggcgg caagatgcag
     7081 cggcgtcgat ttgtttttag tgacggcagg cacattcgga tctgcgccgc gttccagcag
     7141 aagtgtcacc acgggtacat caccccctgt gacggcacgg tacaggaccg accggccgaa
     7201 aagtgacccg ttcaccaatt caggataaga atcgaggaag ctctggagaa atgctttgtc
     7261 ttttctctcg accgcattgc tcaattcgtc gagcagcacc gcctgaaaga aaacctgtgt
     7321 ctgggcgagg gctacacggg tcgccttttc cagagaactc aggtcgtttc tggcttcata
     7381 atcctccctg gctttcttaa tcagatccat gggcgtttcg cccgcggcat tcctggcggt
     7441 aaaatccgca ccccgggtca gcaaaagcga ggctgtgcgg tgaaagcctc cctgaacgat
     7501 cagatgaagc gctgtattgc cgtcattagc aggctcattg acagggatcc ctcgaagtat
     7561 aaaaagctgc agcggccccc aatgcccttt tgcggcactg tagtgcagcg gcagccatcc
     7621 ctgatgatcc cgggcacgca tgtcagcccc tttatcgagc aatgcgttga tattcatcat
     7681 gtgcccgcgg gccgccgcca gatgcagcgg ggtgcatcca tccttatctg ccgcatttac
     7741 actggcacct tgcgcaacaa gaaatttgat ggaatcctca catccaaacc gggcggcaac
     7801 atgcagcggg gcagtacctt tttcgtccac agcattaaca tccaattgct gtccgaccag
     7861 ccagcgtaca gcggaatcgt tctcattgga ggcagccgga aacagcagcg tagccccttc
     7921 gttgtctttg atccccagtg aagcgcctgc ttcagccagc atgcgcgcca gttcataatg
     7981 atcctcttcc agcgcaacaa aaagaggtgt tttgccggtc acatccgtac tgtctacgat
     8041 tgcattggaa tccagaagcg tttttgaaag ctgcatatca cccagagcgg cggcacggtg
     8101 cagcggtgtt tgctccttct gatccgacaa atccggatcg gttcccgatt ccagaagcaa
     8161 ggcggcaatg gcgtggtttc ctttcgctgt cgccagattc aaaggggtac gcccctgctt
     8221 atcctgcgca tttacatcgg cattgttggc aagcaaaaga cggatcatcg cttcgtttcc
     8281 ggcatcggcc gctatatgaa gcggtgtcca tcgtcgtttg tctgtcgcct gtattttggc
     8341 gccattggca agaagcagtt ctgcgaaaac ggtattgttt ttctcagccg ccagatgcag
     8401 tggcgtctgc gccgcgccac tacgtgtatc cacaatagca cctcgttcca gcagaaaacg
     8461 cagcgcagat tctaaattgc gagccgccgc cacatgaatc ggtgcttcgc ctttcgctga
     8521 aaggtatccg atatgggcac catttctgag cagaaaatcc atcatctcca gatcctctgt
     8581 catagtggca tgggctagta aagtccatcc gtaaaaccca ccgtcccgac cgtccgggct
     8641 tgctccgtac ttaaaaaaaa gagccaggta atcctgacgt cccgctttta cagccagaac
     8701 agcgggtgtc tccccttcca gattggaggt ctgacggatg cccgttttca ggataatttc
     8761 gactgcctca gggctgttcg aagccatcga tgcgatgaga ggggtcatcc ccgtcctgga
     8821 gaccagcgaa aattgcgccg gatcctcatc cgcatacttt tgcaggcggg gcaaatcacc
     8881 caaagccgca tcctgaaaaa cgtcgggacg gtagcccatc tcgcgaaaca gtgccgtaac
     8941 atcccagcga tgattttttg ctgccgcatg aaaagccgaa aaataagttt cttcatcata
     9001 actcgtcaga gggctttccc ctttttccac aagaaatctg catgtgtcca ggcttccagc
     9061 atctgcagct tccagaagag gtgcataaat acgctcgtta aggctctcta tgggaattcc
     9121 cttatcaagc agcatcgccg tggtatacac atcatttgcc cgtgccgcca gatggagtgc
     9181 cgtgtttccc agcgcatcct tcgccagcag attgtcgccc ttttctatca gcagatcgac
     9241 aacagcaggc atgttcgacc cggcagcaag aagcagcgca tttttacctg tttcgtcagt
     9301 ctcattatct gcggctccct tttcaatcaa cacaagagaa gcataaatat ttttcgcagc
     9361 tactgcctga aggagacttg taaatacagg cgcctgctcc tgacccggct gaacctctgc
     9421 acttgtcggg atatcagtca aaactgcagg cggctccgcg ccttcctctg tctgtgcaaa
     9481 aagacacgga acagctatca ggcacagtaa tgcaatataa agctgcgggt accgcataag
     9541 ggatctctcc agggttttgg gtgtttaaaa ggacggtatt gacatgactc tccatcgact
     9601 ggccaggaga ggttccttta taacgatcag aatccgctgc gggcatacag gcggaatact
     9661 tcagtataat agccctgcac cgctgtttca attttatcag caaaaccctg tcaccgtatt
     9721 gccttttcac cggaagcggc tgatccattc ctgcaatgat tttcccgatg gcaccgctat
     9781 gaaagcgccc ggcagcagtc ctcgtgcagt ccggaaagtt tggaattcat tttgatttgt
     9841 gacacactat gaacatactg tattctactt gcagtcaata agaggatccc cattttatta
     9901 tgaaacatgt cagacttctg catgccgggg ccttgcgtct caacaacact tttgaggctt
     9961 tgggtgtatc tccggaaacc ggcgatgcac ttcgggcggc tcagaaggat gtgcttgaaa
    10021 agctcgcaca gcaggcgcaa agcttgcccg ctgatatgat cattctcagc ggcggactct
    10081 ttgcgaccct tcctgtccgc gctgatttgc tgctgcgggt tctcgagtgc ctggaacagg
    10141 tcgcccccac tcctgtttat atttttccgg gggcacatga aggacttcat gatctttccc
    10201 cctgggactt tgctctggtg cctgacaacg taactgtttt ttccggcact gcatggtcaa
    10261 gtgttgatca tccgcacctg cccgtaacga tacatggcag gacggacgca gccacgcctc
    10321 ttgatttcag gaatctgcat ttccctgaag acgggcggat ccatattgcc ggtatcgatt
    10381 gcggcaatac atccctggac gaactgcagg aagtcccttc ccgattggcc tatctggcgc
    10441 ttggaacctg ccagataacc gctgatctta aagaaaaggc aggcgtatgc gggtgtgctg
    10501 cagctgcacc gcaggcactc tctttcgatg aatgcggaga gaagcatttc ttcaaagtga
    10561 atataacact ggacgaccac gcttccatac atacaactgt ggaaacactg ccttcctcct
    10621 ccttgctttt tgaaaagcgc agcctcgata tcgctgatct tccttcaccg gatgaaaacg
    10681 cggcgcagct gctcgcactg ccagaacagc atctcaaacg agttctttta aaacttgtca
    10741 tcacagggac taaaaacggg cgtatgatcg atcacctgca cggttttatc aagcacttac
    10801 gtccaaaact tcaacatctg gaagtggaag acatgtcccg gctgaatcct gattctcttt
    10861 cggcggcagg aacagaaatc ctgacacggg atattgcagc acagattttg cagcgcctcc
    10921 gggactgtac cgattatgaa gaaaaacagc ggctggaaat gagtcttcgt cttctgttta
    10981 aggcagttta tgacactcat gccggcctga atgctgaggg attgagcgag ccatgattct
    11041 cacgactctt tcatatctgc atccggaaac gcgcgaatct gtcgtccggg ctttgggtac
    11101 cgagcgatgc ctgctgtatt gtcctgcttc tgaaactgcc cgtttcctca gcacagaaat
    11161 ccatcagcag cagcccgccc ccctggaaac tgccaatgaa gcatctcaag ttttctcttt
    11221 gcccggttac tccagcgttc gactggacta ccggaaaaga attactgcag gtcttcagga
    11281 cagccttttt gctgaagttt cggacagcaa tcccgctccg gatgatccgg acagtgacgc
    11341 tttttttcgg ggtcagcagg atttgtttca cctgtcatta ggagatgcac tgcaggcaga
    11401 acatgaaaaa gaagccctgc tggtgacaca gctcgctccg ctcattgaaa gcggcagctc
    11461 tgccaaaggg ctcaccgaag ccatgaggat attggatgaa ctggcagtgt cgctggggcg
    11521 cggcaaccgc ccgggatctt atctcaccag cctgaaggaa aagatccata aacttgctga
    11581 agagaaaaaa cagactgtgt ccatgccgtc ggagcttttt gcggtcgcca aaacactgaa
    11641 agaaaatcgc tgccgtctga aggaactggg cagtcggcgg gcagcgctgc agaaacagat
    11701 cgatcttctg acagccagaa atgtgaagaa ccttgaaaaa gaaattgctg atctggaatc
    11761 cgcccgggat gcactcggca ccacaccgct cctgagttca gagcagatgg ctgaactgaa
    11821 ccgcatgaca acgctggtca aaaccgttca gtgccaactg gaaaaaaccg gcgatgaact
    11881 gcggctggtg caggagaaac tggagctgct gccgggggaa agcagtcata aatataaagc
    11941 accggcaact tatcctgaag cgcttgagcg ttccattcgg caaacgtcca acaagatcgc
    12001 tgaaaatgaa gtgcggctcg atgaaataca acagcagatg gatcgggaag acaggttcat
    12061 cacgcaggca caggcggaac tcagcagcct acctgacttc agcaaactgc cgcccgatcc
    12121 ggcggacttc ctgaatatcg tgctgcagtc ctgcaaaaat gccatcgagg aacaaaagaa
    12181 tgaagccgag cgctgtgcaa gtctggaaaa agaactggac aggcttcagc gggcatggga
    12241 aatacatggg cccatctttg aacaggcggg tgacttcacc tccatcatgg ttcagaacga
    12301 gcagaagcgg aaagcagccc gggaacgcta tccactgata gcggaagaag tggacaagaa
    12361 gcaaagcatc cgtgatgaac agcaggattt tcagcagagc cagcaatggc tcgccatcgg
    12421 ttgttttctg ctcatgtgtg gtgctgccgg cgcttatttc tttttggaga aaactccgat
    12481 tctgctggta gttgcggctt tgctcatatg tgtggtctat ttttttctgc acgctttttt
    12541 tgctgaccgg caggtacgaa acctgaatca ggccattgcc gaagatcacg ccgaactgga
    12601 tttactgcag gagacaatca atcatagaga cacgtatatt gaagatctga tgcagcagtt
    12661 aggctgccaa tcacccagag aactctcaac tctttatgaa aatcacagag aaatcacgca
    12721 gcagatcatc gacacatcca aagaagtgga acaggcacgc cagctgtgtt tggaatgcga
    12781 ggaacgcgtg cctgctctct ttgaaacgct ctgtgcccat cttgcactgg tggggaaggt
    12841 gcctgaaagc acgagcatca tggattcgta tgttgccgat gccatcgcaa attaccgtcg
    12901 ctgcaaagat gtcaagctcc agctggcgaa ccgacagaac tgccgccaaa gccttttcgc
    12961 acgaaaaacc ttttttgata atgaacagca cgcgcaaaga gacaaattca accagcagat
    13021 ggagcaattc agaacgctta tgcgtgaaaa cggcttcgca caggaagtcg tatacactga
    13081 actggacgac ctttgcgcaa attaccggtc ttttcttgac gatgccctga cttccatgga
    13141 ttcatcggaa ctcctggcac gtcaggcaca aaaactgcag gaaagagaac ggaacgacag
    13201 ggaacaattg gcacgctatg aagaacagcg tcagacgctt ctgaaagcgc tgggcttctc
    13261 cacactggaa gaagccgcag aagcaggtga ccagggtgcc aaattcaaag cgatccaagc
    13321 agaaattgat cttctgaagg caaaactcaa tacttaccgg cagggaccaa tcagagtaca
    13381 gggagagctg ctgctgcctg aagggctggc aggggatgaa gaggaaggtg cactgcggca
    13441 actgaacgcc tccttgaatc aggtggagaa ggaatgcgcc gctgtgcggc agcgctggca
    13501 ggacgcctgg ctccgctaca gcaggctcac cacggagcag cgcccctccg gcgtcatagc
    13561 agaagaactg ctgtggacag aaaaaaacct gcaacaggtg cagaaaatca tcagcgccgg
    13621 agcgcttgtt gtgtctctga ttcaggaaag cctgctgaca gacagaccgg aacgctgtgc
    13681 gcttattgcc gcccgggcta cagagctgct gcagacagca ggtttgtcat ctgcccgggt
    13741 gcagatcgca ctcaatgcgg aaggaaaagc atccattaca gcacacgcag atacagcgac
    13801 agagatgccc tccccactca cgctttatat ggctgtaaaa attgccgcag cacttgttct
    13861 cgcaggcgaa gccggaaaca gacttttcat tgtagacgcg gctttacagc aggaagagct
    13921 gccgacaggg acggatgagt ttttcacttt actggatgcc ctgccccgcc attggcagac
    13981 acttgtggtg actgcaaaca ggacactggt ggaagcgggt gctgcccggg gctggcaaac
    14041 acaaaatctg taatgaaatg gtcggggcgg gcggattcga accgccgacc tcctggtccc
    14101 aaaccaggcg cgataaccgg gctacgctac gccccgaagg cgggatcggt attgtagcac
    14161 gttcctcttt aaagccacaa aaaagaaact gaacgcagaa agaacctgct catggaaagt
    14221 gatatccggc atcttcctga
//
LOCUS       DAOPWB010000080        14067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4433_length_14067_cov_5.230588, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000080 DAOPWB010000000
VERSION     DAOPWB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 14067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14067
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4433_length_14067_cov_5.230588"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..966)
                     /locus_tag="PKN92_08495"
     CDS             complement(100..966)
                     /locus_tag="PKN92_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ48870.1"
                     /translation="MQKKRRRDAFTLIELLVVIAIISILAAILLPALSRARSQARSSQ
                     CVSNLRQLFLANTMYAAEHNGHYVPAAADMYDFMLSGADPDHFGGRLRWHGTRETPNP
                     RTPFDPNTGPLAEYLPDGRVKQCPVFFEYREHNPLENTFEGGAGGYGYNMAYVGSMLS
                     LVKDPVEAVRLGMPDSAIAHPARTIMFTDAAIPLESGLVEYSFVEPPFYVSADYPQGR
                     ADWVNSPTLHFRHYGRANVLWCDGHITSERWGWAPKENIYGASNSRWNVGWFGPENNS
                     LFEAMPRDSTGR"
     gene            complement(969..3113)
                     /locus_tag="PKN92_08500"
     CDS             complement(969..3113)
                     /locus_tag="PKN92_08500"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNZ48871.1"
                     /translation="MRHYGLTFLAALFLFPAAVPAQAAVHPWAARIYDYHAIDPVPGF
                     TDPGKALGKPAGGGVYAPDQSHVYSIGRAGAGQGSFIVFGFEAAIEDHPDNPQGFDFI
                     VFSNGFWVGGSAQRRWIEPALVQVSEDTNGNGLPDDPWYTIPGSRNLAASMLPQGIPD
                     LSPPLSGNVLNPGPGTEEFNWGYAGLAPTMPEYLDNYMRPDNPFETGITEGSGGGDAF
                     DIAWAVDDAGNPAGLSRIHFVRLCAFIAGSHGPLGAVTPEVVGIAAIARDVDTDGDGI
                     LDDYEVRAAGTDPQRRENTVLPLERPLEWGGSPAGTLLGRAEDAQGNAIAFYSSGLRK
                     GKRAYNCAVDIQIPPEIPAASLPDGLLASGCARLFTASEEDFTAAQIQTAEFTLAYDA
                     AQIAGLDEGRLEALRFDGTSFSRTGIENITRDPEENLLSFRCSRPGLFLLASEPGIGD
                     TSPGRVTIPLTAEPVDGVIADGSAVIVVRSDSFLLADGSSPDPAALYTLALFPEHLAS
                     VVNEDLDPDTAGIQLLANSGIITASLRAGTQAGSLTVMLALSDGTASGRLIVPLRAGT
                     AAAPVTLEPLNPRATAPGPIHFTSDPVTDQFGNPVTDGTLLTLEVIGGRCITPDVSSA
                     QPGHQVTVTQHRLSFSILAEAEDGKADTVAVYLKLYADPGKTVLIGSSFHVFDVVTMP
                     LQKAFYPALLLLLFAGIFKAPVRRRRRLPGRK"
     regulatory      complement(3241..3487)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3698..6535
                     /locus_tag="PKN92_08505"
     CDS             3698..6535
                     /locus_tag="PKN92_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48872.1"
                     /translation="MSGTLLLTALLIQTALSAQDDLFIIENRFMRYGVTAEGQNAVLT
                     GLPEEQNVLASPGSACASIKKGGEYYPASQVVKNGTDWHISFGASGAEAVLRPHAEEY
                     FFTMEVISLCGEGVDTLTFVDIPVLEDSPLAACTLALNLKTNVPELPGPMSTLTATAY
                     KRFGFQGAKAAVFAVPRERLRETMQKVVLCSDDIPRSAWGGPWALDVPANYGSYLIDI
                     DGKLTEETADAWIQLAQNMGVGQIDFHMGKTLRFGDLVPDLRAYPRGLDSVKAVVEKM
                     HAAGILAGLHTYAFYIAKDSGWVSPKPDPRLAVARTFTLAKAVDAAADIIPLIEAPDG
                     LSPVTGFQVRNSDTVRLGDELITFSGVCTEPTFALTGCRRGAWGTTAASHETNTQIDH
                     LKECFGLFLPDGESELFVEVAARTAEVFNYCGFDMVYLDALDGSDILAGGEYAWYYAA
                     KFVFELNKRLMRPALFEMSTFMHHLWFARSRMGAWDVPARAPQRFIDIHAAANTESAR
                     MFMPSNLGWWGVFGWSPVQPERSFPEDIDYLCTRSIGHNSSLSFLVGFTPEDWTRSSY
                     IRRLGAKIKQYEERRLSGRVPEEVRATMRIPGDAFELIEDSDGQQSLRQVERFRHIID
                     DGHSSVWTINNPFSAQMPRIRIEALMSASDYDAPEAESIGAFTEQCGFSEVSSQSGVM
                     AAFSLTPDLGKNGLLCASFIVENTSAEDESAWVQVSKTLSPPGDLSRSALGLWVHGDG
                     QGSVLNIQLKNPPHKNNAFSDHYITVDFTGWRYCLLVEPESDRISHYGWPYSQRQSPG
                     TEGIPPFHEIMGNYHFWVDYSDITELNILLNNLPVGKSVNLALSSVRALPLKATELRN
                     PRITIAGKTLLFPVTLRSGQYLELNAAADCVLYDERGEEVASVTPAGAVPELVPGENP
                     VHVFWDTSPADTPPPRARITVFTAGDTLALE"
     regulatory      6735..6825
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            6885..7979
                     /gene="gcvT"
                     /locus_tag="PKN92_08510"
     CDS             6885..7979
                     /gene="gcvT"
                     /locus_tag="PKN92_08510"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNZ48873.1"
                     /translation="MQQTPLFEQYAKHNARVVDFHGWALPLQFAGIIEEHKQVRTEAG
                     IFDCSHMGEFLFTGADAIAALDRLVIGSMTSLAVGKCRYTALLHEDGGILDDGVALRL
                     SEEELLLVTNAGALDSVAALFQQKVPGAKNLSADLAKIDIQGPLAPRILSEFGFECVR
                     SMTFWSGAQLYRKGLDLIITRAGYTGEWGYEIFVPNEGAADLWQELAAHPPVKPCGLG
                     ARDTLRMEMGYPLSGQDLTADRTALEAGLDRFIAWDTDFPGKARMEAQRDSGDYQVLT
                     LLKSADKRAPRHGFELVYNGETAGVVTSGTFGPSVNCGIGLGYVPRALAQPGVMLEAG
                     PRKLPVQIETPPFYKESTGRNRIEAHHLQP"
     gene            7992..8375
                     /gene="gcvH"
                     /locus_tag="PKN92_08515"
     CDS             7992..8375
                     /gene="gcvH"
                     /locus_tag="PKN92_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNZ48874.1"
                     /translation="MNPDTLKYTEDHEWIVEQRGLYVVGITEYAVDELGEITFVELPE
                     VGRRLERGEDAAVLESVKAASDVLCPVTGVVAEVNDILEMEPDLINRDTYGKGWILKL
                     KDVNVEEYEELMDSRSYRGYLKTVE"
     gene            8414..9703
                     /gene="gcvPA"
                     /locus_tag="PKN92_08520"
     CDS             8414..9703
                     /gene="gcvPA"
                     /locus_tag="PKN92_08520"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNZ48875.1"
                     /translation="MDWTPATQKEQQDMLSELGLASTDAFFEAIPASLRMKDWKVPPE
                     GMSEQELRTHLAVLAEANTPAPVSFLGGGYYDHYIPAAVDALAGRSEFFTAYTPYQPE
                     CAQGTLQAIYEYQSAVCRLADMECANASLYDGGTAVYEAAAMACRITRRNRVIVHASL
                     HPVWKEMLETHAAGSVLQVETGDVPVEGSACLIVQNPSFIGDVRDFSDLAEECHAQGC
                     LLVVAFNPVSLGILKTPGAMGADIAVAEGQSLGLPLGFGGPYLGILTARKSHIRKMPG
                     RIAAATTDDRGRRGFVLTLQAREQHIRREKALSNICSNQALCALRALIHLCLLGKAGF
                     EATAQACYAKTEYLKKQMNFLPLLNAHPTFNEFAVRLPKPATEVIEALQPRGFLPGIP
                     LEPFGGEPGDLLIAVTEKRVRAELDAFAAAMKECCHG"
     gene            9696..11156
                     /gene="gcvPB"
                     /locus_tag="PKN92_08525"
     CDS             9696..11156
                     /gene="gcvPB"
                     /locus_tag="PKN92_08525"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNZ48876.1"
                     /translation="MDNRTVFEASVSGRQAVMPSPLDVPAVELPEHLRRSTDLALPEL
                     SELDVVRHFTRLSRKNFGVDSHFYPLGSCTMKYNPKVAEVTASHPGFASLHPQLLSCE
                     SYLRSCQGALRLVFEMEELLAEVGGMEAGCTQPMAGAHGELAGVLLIAAYHRDKGNTG
                     RDTILVPDSAHGTNPASAAIAGFKVREVASTPSGVVDFDAFKACLGEDVAGVMLTCPN
                     THGLFEPHVAEMAALAHEAGALMYYDGANLNAIVGRARPGELGFDVMHYNLHKSFATP
                     HGMGGPGSGAIAVNARLAPYLPGPRVIRSGDRYTLTRPEKSIGRMAPFFGNFLVVVRA
                     YTYLRALGGEGLKEVSRLAALNANYVLHRLKERFPSAYEGLCMHECLLTAEKMEKEQG
                     VSALDIAKALLDRGFHAPTVYFPLTVKEGLMIEPTETESRETLDAFCEAMMDIARLAE
                     EDPDALHRAPENLPVGRLDEVKAARLLDCADLPDKD"
     gene            11243..13813
                     /locus_tag="PKN92_08530"
     CDS             11243..13813
                     /locus_tag="PKN92_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNZ48877.1"
                     /translation="MRNLFLYSLFLLLSCATTAQANEPFSALYLHPDIFADPALSRQE
                     AEALMDRTLDEICACGFTALLPYANTSSGKEHWQVKVEKVEEAARPDTLGLLCAKARQ
                     RGLKIIPVYCVLVSGHDEPAGILKEHPHWALQKEEGTAIGWISPARPEARQWIVERIS
                     ELARHVDADGIMLDYLRFPNEPDVTLDTENLKNFVEAQGDAQADARSTALQEVKESAT
                     TLLMTEISESLRHQKKEIRLGIYSWGAHVTSGHNVAQPWPDWVAAGLLDLINISGYCY
                     TDNYGDAYLDVFRKRLKEAALLAKNASASVDLSFALGVHTSHGTIADAREISRYLASA
                     RTLGYSGVAAFAWGGMKPFLNDIIEAGYFKLSHPLPSLDSSPVRFRMTVDFGADQGQN
                     RGTLFEITDEEGHARIGAGFQGVWNTHYPGDRLSLQLWERPGPDGEAVSFQPLPRPSE
                     GSLHYLFGTADGVHATCYTGDIPFKRWQNEKRQWEAAEGFSFQVGTHRYMAASNVIRL
                     GEKEVFSFEPEKGTAGIYYYGGGHLFFQVRFPGEERKTALYACPWKAETGEVLSLDQA
                     RVQILTVPGEFPYAYGQLNGDVFVATNNGGVFRFSEGQWYLLRPADPKTSFQIYTMIN
                     YYDSLLMGQYPTGELFDIQGDRLVHWEGVPPRPENSSPLFREAQSLAIYRGELYAGIW
                     PWGEVWRLPHREGNWHYAARLFTRPSVSADITAPYEKEMNERSAPVYNIWGQRVTSLV
                     PFGNSLYASTASKNGACFDEEYDFLDSCAQEEYGAVYRLTLPGHAETNIPWTGAPVAF
                     DIQITPEAMTLSADDKEVLQLSRGNNALPYGSRARLHWGQGIFGPLHGEILRAEVEME
                     "
BASE COUNT         3051 a         3866 c         4060 g         3090 t
ORIGIN      
        1 cctgcttcta attttcagta tctgaaaccc gatctccagg aaaagccgtt cctcctgtat
       61 ttgtgctggg aaagggaagc ctgtatttct taaacatctt taccggcctg tactatcccg
      121 gggcatcgct tcgaaaaggc tgttgttttc aggaccgaac cagcccacat tccagcggct
      181 gttggaagcg ccgtaaatat tttctttcgg tgcccagccc cagcgttcgc tggtgatgtg
      241 cccatcgcac cagagcacat tcgcccggcc gtaatgtctg aaatgcagcg tcggagaatt
      301 cacccagtcc gccctgccct gcggatagtc ggcggataca taaaaaggcg gttcgacaaa
      361 actgtactcc accagcccgg actccagagg aatggcggca tcggtgaaca tgatggtgcg
      421 ggcaggatgg gcgatggcgc tgtccggcat gccgagccga accgcttcga caggatcttt
      481 caccagagac agcatggaac ccacataagc catgttatac ccgtagccgc ctgcaccacc
      541 ttcaaaggtg ttctccaacg gattgtgttc ccggtattca aaaaaaacag ggcactgttt
      601 caccctgccg tcgggaaggt attccgccag cgggcccgta ttcggatcaa agggggttcg
      661 cgggttcggt gtttcccggg tgccgtgcca gcggagtctg ccgccgaaat gatcggggtc
      721 ggcaccgctc agcatgaaat cgtacatgtc cgccgctgca ggcacgtaat gtccgttatg
      781 ttccgccgca tacatggtat tggcaaggaa gagctggcgc agattgctga cacattggga
      841 agatcgtgcc tgagagcgtg cccggctcag cgccggaagc aggatggcgg caagaatgct
      901 gatgatggca atgaccacca gcagttcaat aagtgtaaag gcatctcgcc gccgtttctt
      961 ctgcatggct atttccgtcc gggaaggcgg cgccggcgtc ggacgggcgc tttaaaaatg
     1021 cccgcaaaca gaaggagcag cagggcagga tagaaggctt tttgtaaagg catcgtcacc
     1081 acgtcaaaaa catgaaagct gctgccgatc aggaccgtct ttccgggatc agcatatagt
     1141 ttcagatata cggcgacagt gtcggccttg ccgtcttccg cttccgccag aatgctgaaa
     1201 ctcagccggt gctgcgttac cgttacctga tgcccaggct gtgcggagga tacatccggg
     1261 gtaatgcatc ggcctccaat tacttccaga gtgagcagcg ttccatcggt aacgggattg
     1321 ccaaactgat ccgtcacagg atcgctggta aagtgaatcg ggccgggggc ggttgcccga
     1381 gggttgagcg gttccaatgt cacaggcgct gcagcagtac cggcgcgcag cggcacgatc
     1441 agccttccgg aagcggtgcc gtcggaaagg gcaagcatca cagttaaact tcctgcttgt
     1501 gtgcctgcgc gaagggatgc cgtgataatg cctgaattcg ccagaagctg aatgcctgcc
     1561 gtatccggat caagatcttc attcacaaca gatgccagat gttccggaaa aagagcgagg
     1621 gtgtacagcg ctgccggatc ggggctgctg ccgtcagcga gcagaaagct gtccgatcgg
     1681 accacgatga cagcactgcc gtcggcgatg acaccgtcta caggttccgc agttaatgga
     1741 atggttaccc tgcccgggct ggtgtcaccg atgccgggct ccgatgccag cagaaaaaga
     1801 cccggacggc tgcaccggaa gctgagcagg ttttcttcag ggtcgcgggt gatgttttca
     1861 atgcccgtcc ggctgaaaga agttccgtca aagcgcagcg cttcaagtcg cccttcgtcc
     1921 agccctgcga tctgtgctgc atcgtaggcg agggtaaact ctgccgtctg gatctgtgcg
     1981 gcagtgaaat cctcttccga agcagtaaaa aggcgggcac agccactggc aaggaggccg
     2041 tccgggagac ttgcggcggg aatttccggg ggaatctgta tatccacagc gcagttgtag
     2101 gcgcgcttcc cttttctaag cccggaagaa taaaatgcga tggcgttgcc ctgagcatct
     2161 tccgcccggc ccagaagggt gcctgccgga ctgccgcccc attcaagagg gcgttccagc
     2221 ggaagcactg tgttctcccg ccgctgagga tcagtacctg cagcacgcac ttcataatca
     2281 tccagaatgc cgtccccgtc cgtatccaca tcccgggcga tggcggcaat gccgaccacc
     2341 tccggagtaa ctgcgccgag cgggccatgg cttccggcga taaaggcgca cagccggaca
     2401 aaatgaatcc gggaaagccc tgccgggtta ccggcatcat ctaccgccca ggcgatgtcg
     2461 aaggcgtcac cgccgccgct gccttcagta attcccgtct caaaaggatt atccggacgc
     2521 atgtaattat ccagatattc cggcatcgtg ggcgcaagcc ctgcataacc ccagttgaat
     2581 tcctccgtac cgggccccgg attaagtaca ttccccgaca gaggcgggga taagtccgga
     2641 ataccctgag gcagcatcga ggcggcaaga ttgcggctgc ccggaatggt ataccaggga
     2701 tcatcgggca gcccatttcc gttggtatct tcgctgacct gcaccagcgc aggctctatc
     2761 cagcggcgct gtgcgctgcc gccgacccag aagccattgg agaaaacgat gaaatcaaaa
     2821 ccctgcggat tgtccggatg gtcttcaatg gcggcttcaa aaccgaaaac aatgaagctt
     2881 ccctgcccgg cgcctgcccg accgatggaa taaacgtgag actgatcggg cgcgtatacg
     2941 ccgcctcctg ccggtttgcc cagtgctttg ccgggatcgg taaagcccgg aaccgggtca
     3001 atggcatggt agtcgtaaat acgggcggcc caggggtgca cggcagcctg tgccggtacg
     3061 gccgccggga agaggaataa ggcggcaagg aatgttaaac cgtaatgtct catctgtcaa
     3121 gccctcgctt tcgaccggag tccgaggggc acagaagaga atatagaggg aagagaggcg
     3181 ttgccgccgc gcgaaaacca ccgaccttcc cgcgaaagtc ttttgcgtgc acacaggcat
     3241 ccgggccgta gtactatcgg caggtcttcg gacttacggg cttcacatgc gctttcacag
     3301 tacgcctact ggacaccgct tcccgggcaa tcaacacgcc cagtgctttg agaagcatgc
     3361 ctgcggcacg cctccggtgt cggtcgttcc cgattaccgc tgcggggcag tcccggactc
     3421 acaccgggtt ccctcttagc cggaattact gcacaagaaa tcccggaccg atgttttcat
     3481 tatacatgca taaaccgccg gtagcaatct tttaatccgg agtcgcatga tcccgggaaa
     3541 aagagatcaa ccatctttcg gtgcgggcag acaaagtttg tgctatacag gattacagcc
     3601 ggtttgcctg cgtttgccgc aagtgatgcg cagtgctgta gaataatgca ttcacaggaa
     3661 ttgcctttac caccagtccg agccggggag acaatgcgtg tccggtaccc ttctgcttac
     3721 agcactttta atccagactg ccctgtccgc acaggacgac ttgttcatca tcgaaaacag
     3781 gttcatgcgt tacggcgtga cagcggaagg gcagaatgcc gtgcttacgg gattgccgga
     3841 ggagcagaat gtgctggctt cacccgggag tgcctgtgca tccataaaaa aagggggaga
     3901 gtactatcct gcatcgcagg ttgtgaaaaa cggcactgac tggcatatct cctttggtgc
     3961 ttccggtgca gaggcggtgc tgcgtcccca tgcagaggag tacttcttca ccatggaggt
     4021 tatttccctt tgcggggagg gcgtggacac actcactttt gtggatatcc ctgttttgga
     4081 agacagcccc cttgccgcct gtactctggc gttgaatctg aagaccaatg tacccgaatt
     4141 gccgggaccc atgagcacac ttaccgccac ggcgtataag cgtttcggct ttcagggggc
     4201 gaaggcggct gtttttgctg tgccccggga gcgtctgcgt gaaaccatgc agaaggtggt
     4261 gctctgctcc gacgatatac cccggtccgc atggggcggt ccctgggcgc tggatgttcc
     4321 cgccaattat ggctcctacc tcatcgacat tgacgggaaa ctgacagaag aaacagccga
     4381 tgcctggatc caactggctc agaatatggg tgtggggcag atagatttcc acatgggaaa
     4441 gacgctgcgg ttcggagatc ttgttccgga tctccgggca tatccgcgtg gactggacag
     4501 cgtgaaggca gtggtggaaa agatgcatgc tgccggtata ctggcagggc tgcataccta
     4561 tgctttttac attgccaaag actccggatg ggtttccccc aaaccggatc cccgtctggc
     4621 agtggcacgc actttcactc tggcaaaagc ggtggatgct gccgcggata ttattccgct
     4681 tatcgaggcg ccggatggac tctctcccgt caccggattt caggtgcgca acagcgacac
     4741 ggttcgcctc ggtgatgagc tgattacttt ttcgggcgtg tgcactgaac cgacctttgc
     4801 ccttacaggc tgcagacgcg gcgcctgggg tactaccgca gcctcccacg aaacaaatac
     4861 gcagattgac catctgaaag agtgcttcgg tctttttctg cctgacggcg aatcggagct
     4921 ctttgtcgaa gtggcagccc gtacagctga ggtttttaat tactgcggtt ttgacatggt
     4981 ctatctggat gcactggacg gctcggacat tctggcgggc ggtgagtacg cctggtacta
     5041 tgctgcaaaa tttgtatttg aactgaacaa aagactcatg cgcccggcac tttttgaaat
     5101 gagcacgttc atgcaccatc tgtggtttgc ccggtcgcgc atgggtgcct gggatgtgcc
     5161 tgcccgggct cctcagcgtt ttattgatat ccacgctgcg gcgaatacgg aaagtgcacg
     5221 catgtttatg ccttccaatc tgggatggtg gggcgttttc ggctggagcc ccgtgcagcc
     5281 tgagcgcagt tttccggaag acatcgatta tctgtgcacc agaagcatcg gccacaattc
     5341 aagcctctct tttctggtgg gatttacccc ggaagattgg acacgctctt cttatatccg
     5401 ccgtctgggc gccaaaatca aacagtatga agaacggcgc ctgtcggggc gtgtcccgga
     5461 agaggtgcgt gcgacaatgc gtattccggg ggatgctttt gaactgatcg aagactcgga
     5521 cgggcagcag tccttgcgcc aggtggagcg cttcaggcac atcattgatg acggacacag
     5581 cagtgtgtgg acaatcaaca atcccttttc cgctcaaatg ccccgtattc gcatagaggc
     5641 gctcatgtcc gcttccgatt atgatgcgcc ggaagctgag agcatcggcg cttttacgga
     5701 acagtgcggg ttcagcgaag tcagctccca atccggcgtg atggcagctt tctctctaac
     5761 gccggatctt ggtaaaaacg gcttgctctg tgcttctttc attgtggaaa acaccagtgc
     5821 tgaagacgaa tcggcgtggg ttcaggtgag caaaaccctg tcccctcccg gagatctgag
     5881 caggagtgca ttgggcttgt gggtacacgg tgacgggcag ggatcggtac tgaacatcca
     5941 actgaaaaac ccgccgcata aaaacaatgc tttcagtgat cactacatca ctgtcgattt
     6001 tacaggctgg cgctattgcc tgctggtcga gccggaatcg gatcgtatca gtcactacgg
     6061 atggccctac tcacagcggc aaagccccgg taccgaggga ataccgcctt ttcatgaaat
     6121 catggggaac taccatttct gggtggatta cagcgacatc accgagttga atattctgct
     6181 gaacaatctt cctgtcggca aaagtgtaaa ccttgcgctc agttctgtcc gggcgctgcc
     6241 gctgaaagct acggagctcc gcaatccccg tatcaccatc gcaggaaaaa ccctgctttt
     6301 tccggtcacg ctcaggagcg ggcaatacct ggagctcaac gctgcggcgg attgtgtgct
     6361 gtatgacgaa agaggggagg aggttgcctc cgtgacaccg gccggtgccg ttcccgaact
     6421 ggttccgggt gaaaatccgg tacatgtttt ctgggacacg tcgcccgctg atacgcctcc
     6481 gccgcgtgcc cgcataaccg tatttacagc aggtgacaca ctggcattgg aataacactt
     6541 tttgagaggc ctgctgaaac agaaacagca gaaggttcag caggaagaaa gggcattgga
     6601 taccgggcaa tcgtaccgga tgcgacgctc agaaagttat ccggtactga attcggacat
     6661 ctttccgaaa tagccgggtg accggcggta tcttgcaatt tttccggaaa tatggcatta
     6721 tactgccgga ttccggcgcc tgccgtaact ggcgagatca agcgtggagg caaccacgat
     6781 ggagcggtgg agcgcgttat agtgccgttc gcctgggcat ctcctgtgtg tcttttgggc
     6841 aagacacgca tgggcagtga tggatattcc gtgaaaggcg aactatgcag cagactcctc
     6901 tttttgaaca atatgcgaaa cacaatgccc gtgtggtgga ttttcacggc tgggcactcc
     6961 cgcttcaatt tgccggtatt atcgaagagc ataaacaggt gcgcaccgaa gccggcatct
     7021 tcgactgttc ccatatgggc gaattccttt ttacgggtgc cgatgccata gcggcgctgg
     7081 accgtcttgt aatcggcagt atgacgtcgc ttgctgtggg caaatgccgg tacaccgccc
     7141 ttctgcatga agacggtggc attctggacg acggtgtagc cctccgcctg tctgaagagg
     7201 aactgcttct cgtgaccaat gccggggctc tggatagtgt ggcggcgctg tttcagcaga
     7261 aagtgcctgg tgcgaagaat ctttccgctg atctcgcaaa aattgacatt cagggcccgc
     7321 tcgcaccccg gattctttca gaattcggtt ttgaatgtgt acggagcatg actttctggt
     7381 cgggtgcgca actgtaccgg aagggactcg atctgatcat tacccgtgcc ggatatacag
     7441 gtgaatgggg ctatgagatt ttcgtgccca atgaaggcgc tgccgaccta tggcaggaac
     7501 tggcggccca tcctccggta aaaccctgcg gtctcggcgc acgtgatacc ctgcgcatgg
     7561 aaatggggta tccgctgagc ggacaggatc tcaccgcaga ccgcaccgca ctggaagcgg
     7621 ggcttgaccg ttttatcgcc tgggataccg attttcccgg caaagcacgg atggaagcgc
     7681 aacgggatag cggggactat caggtactga cactgttgaa aagtgcggac aaacgggcac
     7741 cccggcacgg ctttgaactg gtttataacg gcgaaacagc aggcgtggtg accagtggta
     7801 ccttcggtcc atcggtcaat tgcgggattg gtctggggta tgtgccccgg gcactggcgc
     7861 agccaggtgt catgctggaa gcggggccac ggaaactgcc cgtacagatt gaaacgccgc
     7921 ctttctataa agagtctaca ggacgtaaca gaatcgaagc acatcatctg caaccctaat
     7981 ggaggaaaac catgaatccc gatacgctga aatacacgga agaccacgag tggatcgttg
     8041 agcagcgcgg tttgtacgtt gtcggcatta ccgagtatgc cgtggatgaa ctcggcgaaa
     8101 ttacttttgt cgagctgccc gaagtcggcc gccgccttga gcggggtgaa gatgcggcgg
     8161 tgctggaatc cgtaaaagcc gccagcgacg ttctgtgccc ggtgaccggt gttgtggcgg
     8221 aagtcaatga catcctggaa atggaaccgg atctgatcaa cagagacacc tacggtaaag
     8281 gctggatttt gaaactcaaa gatgtgaatg tggaagaata cgaagagctt atggattccc
     8341 gcagctaccg cggctatctg aaaacagtgg aatagcacaa aatccagatt tttgacttcc
     8401 ggagaaacca ttgatggact ggacgcccgc cacacaaaaa gaacagcagg atatgctgag
     8461 cgaactgggg cttgcctcaa cagatgcttt ttttgaagcc atcccggcca gcctgcgcat
     8521 gaaagattgg aaggtgccgc ctgaaggcat gagcgagcag gaactgcgta cgcatcttgc
     8581 cgttttggcg gaagccaaca cacccgcacc ggtttccttt ctcggcggcg gatactatga
     8641 tcactatatc cccgccgccg tggatgcgct ggcgggcaga agcgaattct tcaccgctta
     8701 cacgccctat cagcctgaat gcgctcaggg aaccctgcag gcgatttatg agtatcagtc
     8761 ggcggtctgc cgcctggcgg atatggaatg cgccaatgct tccctctatg acggcggtac
     8821 tgccgtgtat gaagcagcgg caatggcatg caggattaca cggcgcaacc gggtgatcgt
     8881 acacgcttcc ctgcatccgg tctggaaaga gatgcttgaa acccacgctg ccggctcagt
     8941 gcttcaggtg gaaactggcg atgtccccgt ggaaggaagt gcctgtctga ttgtgcagaa
     9001 tccttctttt atcggtgatg tccgcgactt ctccgatctt gcggaagaat gtcatgcgca
     9061 gggatgcctg ctggtagttg cctttaaccc ggtctcactc ggaattctga aaacgcccgg
     9121 cgccatgggc gctgatattg ccgtggcgga agggcagagc ctcgggcttc ctctgggttt
     9181 cggcggccct tatctcggca ttctcaccgc cagaaaaagt cacatcagga aaatgccggg
     9241 gcgcattgca gcggcaacga ctgatgacag gggcaggcgc ggttttgtcc tgacgctgca
     9301 ggcaagagaa cagcatatcc gccgggagaa agcgctgtcc aatatctgct ccaatcaggc
     9361 gctgtgtgcg ttgcgggcgc tgattcatct ctgccttctg ggcaaagcgg gctttgaagc
     9421 aacggcgcag gcttgctatg ctaaaacgga gtatttgaaa aaacagatga actttcttcc
     9481 gttgctcaat gcacacccca ctttcaatga atttgcggta cgtttgccga agcctgcaac
     9541 ggaagtgatc gaggccttgc agccccgggg attccttccc ggtattccgc tggagccctt
     9601 tggcggggag cccggagacc tgctgatcgc cgtaaccgaa aagcgtgtcc gagcagaact
     9661 ggacgccttc gccgcagcca tgaaggagtg ctgtcatgga taaccgcacc gtatttgaag
     9721 caagcgtttc cggtcgccag gccgttatgc cgtctccgct ggatgtgcct gctgtggaac
     9781 tgccggagca cctccgtcgc agcaccgatc tggcgcttcc cgaattaagc gaactggatg
     9841 tggtgcgcca cttcacgcgg ctctcccgca aaaatttcgg cgtggattcc catttttatc
     9901 cgctcggctc ctgtaccatg aaatacaatc ccaaagtggc ggaagtaact gcttcccatc
     9961 cgggtttcgc atcgctccat ccgcagctgc tttcctgcga atcctacctc cggtcctgtc
    10021 agggtgcttt gcgcctcgta ttcgaaatgg aggagcttct tgccgaggtc ggcggcatgg
    10081 aagccggatg cacccagccg atggcaggtg cccatggcga actcgccggt gtgctgctca
    10141 tcgccgccta tcaccgtgac aaagggaata cgggccggga taccattctg gtacccgact
    10201 cggcacacgg aaccaatccg gcaagcgccg ccatagcagg cttcaaggtg cgcgaggtgg
    10261 cgtccacgcc gtcgggtgtg gttgattttg atgcctttaa agcgtgcctg ggtgaagatg
    10321 ttgccggagt catgctgacc tgtcccaata cgcacgggct tttcgagcct catgtggcag
    10381 agatggcggc actggcgcat gaagcgggtg cgctcatgta ttacgatggc gccaatttga
    10441 atgccattgt cggcagggca cgtcccggcg agctgggttt cgatgtcatg cattacaatc
    10501 tgcataaaag ttttgcgaca ccccacggca tgggcggtcc cggatccggc gcgattgcag
    10561 ttaatgcacg gctcgccccc tatctgcccg gaccgcgtgt tatccgcagc ggcgaccgtt
    10621 acacgctgac ccgtccggaa aaatccatcg gaagaatggc tccttttttc gggaattttc
    10681 tggtggtggt ccgtgcctat acctacctcc gtgcattggg cggtgaaggc ctcaaggaag
    10741 tcagcagact ggcggcgctg aatgccaact atgtgctgca caggctgaaa gagcgtttcc
    10801 cttccgctta cgaaggactc tgtatgcatg agtgcctgct gacagcggaa aagatggaga
    10861 aagagcaggg cgttagcgca ctcgatatcg caaaagcgct tctcgaccgg ggctttcatg
    10921 cacccaccgt gtatttcccg ctgactgtga aagaagggct gatgattgag cccacggaaa
    10981 cagaatcccg cgaaacgctg gatgcttttt gtgaggccat gatggatatt gcccgactgg
    11041 ccgaagaaga tcccgatgcc ctgcaccgtg caccggaaaa cctgcctgtg ggacggctgg
    11101 acgaagtgaa ggctgcccgc ctgttggatt gcgccgatct gcctgacaaa gactgattct
    11161 tcaggcaggt gcagcgccgg agctgaaaac ggtgcagtcg ggtcatgcca ggttccccgg
    11221 tcaggggtga ggaggagatg tcatgcgaaa cctgtttctg tattcgcttt ttctgttgct
    11281 gtcctgtgcc acaacggcac aggccaacga gcccttctca gcgctttatc tgcatcctga
    11341 catttttgcc gatcctgcgc tgtcacggca ggaggcggaa gcgctcatgg atcgcactct
    11401 cgatgagatc tgtgcctgcg ggtttactgc tcttctgcct tatgcaaaca cctcctccgg
    11461 gaaagagcac tggcaggtga aggtcgaaaa ggtagaggag gcagcccggc cggataccct
    11521 aggacttctc tgtgccaaag cacgccagcg cggattaaaa attataccgg tctactgtgt
    11581 tctcgtttcc gggcacgatg aacctgccgg cattctgaaa gaacacccgc actgggcact
    11641 gcagaaagaa gaagggacgg cgatcggctg gatttcgcct gcccgtcccg aggcgcgtca
    11701 atggattgtg gagcgcatct ccgaattggc tcgccatgtg gatgccgacg gaatcatgct
    11761 cgattacctg cgttttccca atgagcctga cgtaaccctt gatactgaaa atctcaagaa
    11821 ctttgtggag gctcagggtg atgcgcaagc ggatgcaagg agcaccgccc tgcaggaagt
    11881 caaagaatcg gccacgaccc tgctcatgac ggaaatttcg gagagtttgc ggcatcagaa
    11941 aaaagagatc cgcctgggta tttacagttg gggagcacat gtgacttcag gacacaatgt
    12001 ggcgcagccc tggcctgatt gggttgctgc agggctcctc gacctgatca atatttccgg
    12061 ctattgctat accgataatt acggcgatgc ttatctggat gttttcagaa aacgcctgaa
    12121 agaagccgcg ctgcttgcga agaatgcatc cgcctctgtg gatctatctt ttgccctcgg
    12181 cgttcacacc agccacggca ccattgctga tgccagagag atctcccgtt atctcgccag
    12241 cgcccgcacc ctcggctatt ccggagtcgc cgcttttgcc tggggaggga tgaagccctt
    12301 tctgaatgat attatcgaag cgggctattt taagctcagt cacccgcttc cttctctgga
    12361 cagctccccg gtccgattcc gtatgacggt ggacttcggt gctgatcagg gacagaatcg
    12421 gggtacgctt tttgagatca ccgacgaaga gggtcatgcc cgtatcggtg ccgggtttca
    12481 gggcgtttgg aacacccatt atcccggcga ccgcttgtca ttgcagctct gggagcgccc
    12541 cgggcccgac ggagaagcag tctcctttca gccgctgccc cgcccttcag agggatctct
    12601 gcactatctc ttcggtacag ccgatggcgt gcatgccacc tgttacaccg gagatattcc
    12661 cttcaagcgc tggcagaatg aaaaaagaca atgggaagca gcagagggct tttcctttca
    12721 ggtcggcacg catcgctaca tggccgcaag caacgtgatc cgactgggag aaaaggaagt
    12781 gttttccttt gaacctgaaa agggcacggc aggtatttat tattatggag gcggtcatct
    12841 ctttttccag gtgcgttttc cgggagagga acggaaaaca gctctctatg cctgcccgtg
    12901 gaaggcggaa acgggtgaag tgctttcttt ggatcaggca cgtgtgcaga tacttacggt
    12961 gccgggtgaa ttcccttatg cgtacggaca gctgaacgga gatgtcttcg ttgcaacgaa
    13021 caacggcggg gtgtttcgct tctctgaggg gcaatggtat ctcctgcgcc ccgctgatcc
    13081 caaaaccagt tttcagattt ataccatgat caattattat gacagcctgc tgatggggca
    13141 gtatccaaca ggcgaacttt ttgatataca gggggaccgg cttgttcatt gggagggcgt
    13201 gccgccccga cccgaaaaca gttccccttt atttcgggaa gcgcaatcac tggcgatcta
    13261 ccggggtgaa ctctatgcgg gcatctggcc ctggggtgaa gtctggaggc tgccccatcg
    13321 tgaaggcaac tggcactatg ccgcccgcct ttttacccgg ccgtccgtca gtgccgatat
    13381 caccgcaccc tatgaaaagg agatgaacga gcgcagcgcc cccgtgtaca acatctgggg
    13441 tcagcgtgtc acgagcctcg ttcctttcgg caacagcctc tatgcctcta cagcaagcaa
    13501 gaacggtgcg tgtttcgatg aggaatatga ttttctggat tcctgtgccc aggaagaata
    13561 cggtgctgtc tacagactca ccctgcccgg acatgcggaa acaaatatcc cctggacagg
    13621 agcgcccgta gccttcgata tccagattac cccggaagca atgactctgt ctgcagacga
    13681 caaagaggta cttcagctct ctcgcggcaa caatgctctg ccttacggtt cccgagcccg
    13741 cctgcactgg ggtcagggca tctttggtcc gctgcacggc gaaattcttc gggcagaagt
    13801 cgaaatggag tagcttttca ctgttggatt cgcttctggt ctacagcgac acccgtccac
    13861 agcgtcaccc cgttcgcgtc gcaccctcgc ctacagcgac acccgtccac ggcgtctatt
    13921 tccgcactcc ctgccacgct tgccttttct cgtcattcct gcgaaggcag gaatcttaat
    13981 cagggtgtgg acgtcttgat ctgcatatcg ccctgatcaa gattcctgcc ttcgcaggaa
    14041 tgacgagaaa aggcaagcgt ggcaggg
//
LOCUS       DAOPWB010000081        14000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4457_length_14000_cov_5.827106, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000081 DAOPWB010000000
VERSION     DAOPWB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 14000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14000
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4457_length_14000_cov_5.827106"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKN92_08535"
     CDS             complement(<1..653)
                     /locus_tag="PKN92_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48878.1"
                     /translation="MLTPPRFIFCALLALVLAGSVLPAHAADCDPDTTKPVLTIVQRE
                     DRSSYQNYPGVFSGWFYDAVPCGSDWAAEGPSSISALDNCDGAVPVTVVIHDVQVVGM
                     SPVFTPIANLGLTESTWTTRPGMYRLIYSAKDGAGNSSSETVGAQVQEGCAEEGEPVE
                     GEPVVEGEPEPGAPVITIQHREENGGYCNISLLDEPVFVYDSIECGADWASVGPLSVT
                     "
     gene            complement(943..4173)
                     /locus_tag="PKN92_08540"
     CDS             complement(943..4173)
                     /locus_tag="PKN92_08540"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNZ48879.1"
                     /translation="MSVFSESMIVLGPASLGFCASAAADLQVLELRTEYRINPEGIEE
                     RQPRFNWQLQSEERNCLQHAYQILVADSPENLANDRGTLWDSGKVDSGESTFIVYAGE
                     PLQSRMRCFWKVRVWDNQNRMSAWSDTASFSLGLLCERDWHARWIGFNAIGAEDLDAA
                     AVARIESARWIGTAPSEDENAESAKTIYFRKQFQLPEDAVIVNASLWVTAEASCQGWV
                     NGYQLNQGRPQMKDWHEVYPHLVAALLQPGENVLAFSVTAGKAQSGLIAALKITLRDG
                     NVIDITTDSSWLCKTEEPADSWTTGASDTEGWQPVTDIAGYNEAPWEGTAPDLTQFRP
                     VAQLRKDFSTRAGIKEATLYATALGLYEFRINGEKAGNCFFTPGWTDYKKRLYYQRFD
                     VTGLIRPDGENVLGALLANGWYAGHIGLKGSAAYGTKPALYAQLEIDYEDGNRQIVVT
                     DDSWRAAFGEIRGSDLLQGEVRDFRKALSGWDMPGYDAGAWAAVSMDAPEVVPEKLQA
                     YPGAPVKAFECLKAQSCNEPRPGVFVYDLGQNMVGWPRIELEGRSGQLIRVRHAEMLQ
                     ENGTLYTDALRKAEATDFYYLSGQGREILKPSFTFHGFRYVEIRGIEQALPLEAVTGQ
                     VVNADVDIVADFRSSNPLLNQLAHNIVWGLKGNYLEAPTDCPQRDERLGWTGDAQFFM
                     PTALYTADIGAFFTKWLVDLIQDSQQADGSFAHVAPDVIHEGGAVAWGDAALICPWLM
                     YQFYGDVRVIEKHYDQMVLGMAFLEKTSKDFIREKLGFGDWVNLGGGAKAEVICTAYY
                     AYLADIMSQMAALIGKEDDAAAYAELHKNIKEAFIRNFVGDDGSILESSQTGYALAFA
                     FDLIPEKLKEAAARHYVEEIARFDWHLATGFIGTPRLLPSLTRAGRDDVAYQLLMNTT
                     YPSWLYQVTLGATTMWERWNGWTPEKGFETPGMNSFNHYAFGAVGEWLYSRVAGIRPE
                     SPGFARMRIAPLPGGGLTEARLDYRSIRGLIHAGWEVKDGILSLDVTVPANAEALVCI
                     PTSDPESVKEGGSTLEAAGIPVAESAADRVTCAVGSGTYHFSATVR"
     gene            complement(4252..4443)
                     /locus_tag="PKN92_08545"
     CDS             complement(4252..4443)
                     /locus_tag="PKN92_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48880.1"
                     /translation="MLHALQNGLLGGGIFFLLVMFWLGVQYVIRRGKGLAPDCDVLDD
                     PDHACLHCSKHKGCGSARH"
     gene            complement(4449..5030)
                     /locus_tag="PKN92_08550"
     CDS             complement(4449..5030)
                     /locus_tag="PKN92_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HNZ48881.1"
                     /translation="MNPESLPSVFINACIVNNFVLSMFLGICPFLGVSNKKDTAVRMG
                     IAVTFVMLVCSVCTYGINFVLDSVDAPYLKLICYIVVIASAVQLVEMVIKKYSPALFR
                     ALGIFLPLITTNCAILALAMFQTQKEYNFIQCIVYAVGAGAGYTLALIIMAGMREKLE
                     LADVPDVAQGAAITLILAGILSLAFMGFAGLGG"
     gene            complement(5043..5699)
                     /locus_tag="PKN92_08555"
     CDS             complement(5043..5699)
                     /locus_tag="PKN92_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNZ48882.1"
                     /translation="MSMEKKGPSSWDVFFAGFWRDNPVFVMLLGTCPTLAVTNSVINC
                     FAMGVSTLFVLVMSGILISMLRNFIPKQVRIASFIVVIATFVTIVDYLIQSISLDLYD
                     SLGAFIQLIVVNCIILGRAEAFASRNGVMRSVLDAIGMGIGFTIALLCLGVVREVLGN
                     GTLLMNTPYEISLFGPRFEPWSVMILPPGGFFVLGVELLFFNWWRKRKEARALAQAKA
                     "
     gene            complement(5696..6379)
                     /locus_tag="PKN92_08560"
     CDS             complement(5696..6379)
                     /locus_tag="PKN92_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNZ48883.1"
                     /translation="MSVSETTPPVDQKKDNAGSLAMLRTLGAISFVSGILLSVVYQAT
                     FSRIQTNTEERIRSTVLELVPGSVIQKEFTFFSEASPDVPRTVYAGYDASGTFLGVAL
                     EAIDLGGYGGEIKLLYGYIPDEHKIVGTKVLLCKETPGLGDKIKTDEHFQKNFESLSV
                     PLDDSRQHLLYPLKLVKAGAGGGPGQIEAISGATISSTAVVNGMKESTEVVLPLVHQH
                     LQELKEQGK"
     gene            complement(6376..7461)
                     /locus_tag="PKN92_08565"
     CDS             complement(6376..7461)
                     /locus_tag="PKN92_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNZ48884.1"
                     /translation="MSTEARPLIIGSSPHIRVKQSVPDIMFHVALALLPVAAFAVWSF
                     GLSALLLLLTTTAACVATEHTACLIARRSTTIRDWSVVVTGLILGLTLPPSTPLWMAV
                     LGSIFGVSVTKSLFGGLGYNCFNPALVGRAFLQIAFPIPLTTWTPPDLPGRFSSLLPT
                     TLTFPFLRADTAALADYIRTALASGGVDGWSGATPLSMWKFAEVPQFESLPRLFLGSI
                     AGSTGETATWLIILCGLYLIARNMMNWRIPAGMLLGAFLLSGAFWLSDKTHYPSPMFM
                     LFSGGLMFGALFMASDMVASPMTSAGVWIYGFFIGMVTVIIRIFGALPEGVMFAILLG
                     NAVSPLITSATQPRTYGLVRQKRWIKQ"
     gene            complement(7458..8789)
                     /gene="rsxC"
                     /locus_tag="PKN92_08570"
     CDS             complement(7458..8789)
                     /gene="rsxC"
                     /locus_tag="PKN92_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNZ48885.1"
                     /translation="MKSYVNSFPHGVHPPDCKYTADAVIETLPLPPEVTILLSQHIGA
                     PAKALVAKKSKVMRGEPIAEAVGFVSVPMHAPIAGTVTAVDYALNPRGEMAQAIKIKI
                     DPDADQEEIYGTPRDLASLTPDELIRAVQETGTVGLGGAAFPTHVKMKPPAGKSVDTL
                     IVNGCECEPYLTTDFRIMVEESDKIITGIQLVMEAMGAKEAFIAIENNKPEALESMAK
                     AIPAGAPISVRSLMTKYPQGAEKMLSRAVLHKDVPSGGLPSDIGVGCFNVATLLQLGQ
                     LLPKGQGLIERVITVSGGGVERPGNYRIPLGTPIRYIMEQVGIRPEARKIISGGPMMG
                     MSLSSQDIPLTKGMSGLLVLTEEEMRDGGGTVYPCIKCARCVDACPIFLNPSLLGLLA
                     RKARYEEMAEEHHLNDCFECGCCTYVCPSNIPLVQYFRIAKAMNREREAAK"
     gene            complement(8794..9720)
                     /locus_tag="PKN92_08575"
     CDS             complement(8794..9720)
                     /locus_tag="PKN92_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNZ48886.1"
                     /translation="MLISALSIAGGVMLLLGLLLAAVLAIANKKLYVYEDPRIDEVEA
                     QLPSANCGACGFAGCRAFAEAVIRGEAAPGQCTASSADTVESIAAYLGVDAGVGEKRV
                     ARLACSGGDNVARRFAHYEGRYSCRAASLVAGGGKHCTFGCIGYGDCVAVCPFDALHM
                     NEHMLPVVDEAKCTACGNCVVECPKHLFSIHPISHQLWVSCSTRLRGKDAKAACAVAC
                     IGCGLCAKAAPEAVSMDGNLPVVDYGKNEFAVREAIENCPSGAILWVERDGRRVLGVK
                     AAAALEKVLNPPEAPLKKTSASEAEQKSEVSG"
     gene            complement(9887..10972)
                     /locus_tag="PKN92_08580"
     CDS             complement(9887..10972)
                     /locus_tag="PKN92_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNZ48887.1"
                     /translation="MNRLFSLPFALAGLALVFLALSSFADDAAPAAAPAEAAPAEAAP
                     AAAPPADVRQVQMKVWISETSEGGVRDLGANLDFTRFVRGQEMTGSVQQVTTSTINPL
                     STFGTVAMPYPHQALFGAPLRPDLNPATTQLEAYPGLGLEFSILDADYGTIDGIFQSL
                     ETRSDLDLISKPEIMVANTLPATIKAGGQVPFQDVTFPSAATRLQVSWRDIGVNLNLT
                     PTIMPNNFVQLAITQLEVSDILSYENLRGADLPVFSTRSQSGVVFVPDGTTLVVGGLT
                     SRTVRKTERRVPVLGSVPLLGIPFRSRKSEAQVTSLLVFVSPTIVDMRAPTPQAKTAM
                     NFWRERGGEWTNRPRIDREVDAMQTGF"
     gene            complement(10972..12090)
                     /locus_tag="PKN92_08585"
     CDS             complement(10972..12090)
                     /locus_tag="PKN92_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48888.1"
                     /translation="MIKRSVYILAALLLCGFAQGQDPQQVNVSVKIIEFQTTKGAESG
                     ISAYFKKRITPRPYGLVSTGPGIIDAASLAFPNRSTAGITLFLDQISTYYGDIEMILQ
                     ALVDQNRAFILSQPKVMVSVGAAAPTIIKTTQDVPYENTVVVGVNAVQTTAFRPTGVT
                     LTVSALQVVDDDGNPNTTEDQYIQLKLTAEINEEGQRITIALADTFASTGAVVSRSTN
                     AISVPEFVSRSLDTTVWVKNNQVLILGGLYRNTKNKNVSTLPWLTQGEDLVNNTLERL
                     SPFSETPDIPLTSALGNKRSSETRRELVFMVKADIWRQAFTLYEGFGAPGGEDAASEE
                     EGEEDIVHPTPPSGFIGSLFGGSNTVPSGVSDSLGGRE"
     gene            complement(12106..13137)
                     /locus_tag="PKN92_08590"
     CDS             complement(12106..13137)
                     /locus_tag="PKN92_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48889.1"
                     /translation="MKQSINQCFRAAVLVFPAVLWLLPLFSVVAGCATPGSASGQQAS
                     RPVQVGDVIARAPESNSGSAAKELAAARRMIKAGESSLVIPRLQTLVNTYPGDASAIE
                     ARFFLGKAFYTVGAYSDAHRWLQEYLVLVPEGENSAEGRQLLEKLAGGGESGKAAGDD
                     AFAALEAAIAADPEAMAPRLDYANTLWEKGRYDQAGKAYDELLKRWPQLASDAAVSRR
                     VERDASGRLVILTPAEMERRYQEAEPLLIYNVSSFRSGRFEGWPTTAREKFYNVAGQA
                     VNQSEAPLEDVRITVTIYGFGQMVYDTQTISLGVLRPREIRAFNAQFSRFDDIHNITR
                     HECVGSYRR"
     gene            complement(13154..13477)
                     /locus_tag="PKN92_08595"
     CDS             complement(13154..13477)
                     /locus_tag="PKN92_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48890.1"
                     /translation="MSIPPEAVDDQRIFWGTASSFEKPGSVDYNAVVSATTEYKEAVK
                     AEKNTAQYWLFISKANDRAIRAISSVGSETDFDLIVLKGYLEGLTPPIATEDITELAL
                     AKLSK"
BASE COUNT         3474 a         4123 c         3459 g         2944 t
ORIGIN      
        1 gtgacagaga gaggccctac tgatgcccag tctgctccgc attctataga gtcgtaaaca
       61 aaaacaggct catcaagcaa agaaatattg caataacctc cgttttcctc ccgatgctgt
      121 atcgttatga ctggagcacc cggttcaggc tcaccttcta ccacgggctc gccttctacg
      181 ggttcccctt cttcagcgca gccttcctgc acctgtgccc cgactgtctc acttgatgaa
      241 ttccctgccc cgtctttggc gctgtagatc agccggtaca tgccgggccg ggtcgtccag
      301 gtactctctg taagacctaa attggcaata ggggtaaaaa ccggcgacat accgacaacc
      361 tgcacatcgt gaatcacgac cgtgaccgga acagccccgt cacaattgtc aagagcgctg
      421 atcgaagacg gcccttcagc cgcccagtcc gatccgcagg gaactgcatc ataaaaccat
      481 ccggaaaata ctccgggata gttttgatac gaagacctgt cttcccgttg tacaatcgtc
      541 agtacaggtt ttgttgtatc cggatcgcaa tccgccgcat gcgccggcaa aacactgcct
      601 gccagtacca gcgccagcag tgcacaaaaa atgaaacggg ggggggttaa catggcaaat
      661 gctccattac cgattttcaa aaatgaacgc aactcaacct tccggctgat tgccctaaag
      721 gttcatatca gccactccga tatttcttcc cttacactgg gaagttctta ttatagaggc
      781 atcgtgaaat ataagcaagt caaaaatata attattatag aaacgaagat agtaataaat
      841 cttcgatgag acaggcgaaa aaaacatgct tcatgctttt agtcatttac aaaaaaaaat
      901 cccgggcaga agcaacgctg cccgggagca ggtttttaat ttttagcgga cagtggcaga
      961 gaaatggtac gtgcctgaac cgacagcaca ggtaacacga tctgccgcac tttctgcaac
     1021 aggaatacct gccgcttcaa gtgtacttcc gccttccttc actgattccg gatctgaagt
     1081 cgggatgcat accagggctt cagcattggc gggaacagtc acatccagag ataaaatacc
     1141 gtctttcact tcccagcccg catgaataag cccgcggatt gaccgatagt ccagccgggc
     1201 ttccgtaagc ccgccgccgg gcagcggcgc aatgcgcatg cgggcaaatc cggggctttc
     1261 aggccggata cccgcaacac ggctgtacag ccactcaccc acggcaccaa aggcgtaatg
     1321 attaaaagaa ttcatgcccg gtgtctcaaa gcccttttca ggagtccatc cgttccagcg
     1381 ttcccacatg gtggttgcgc cgagagtcac ctgataaagc caggagggat aggtcgtatt
     1441 cataagcagc tgataggcga cgtcatcccg ccccgcccgg gtcagcgacg ggagaagccg
     1501 gggggtaccg atgaagcccg tggcgaggtg ccagtcaaag cgtgcaatct cctccacata
     1561 atgccgggca gcggcctctt tcagcttctc cggaatcaga tcaaaggcga aggcaagggc
     1621 atagcctgtc tgactgcttt ccagaatact cccgtcatca ccaacaaaat tccggataaa
     1681 ggcttctttt atatttttgt gcagctcagc ataggcggcg gcatcgtctt ccttgccgat
     1741 cagtgccgcc atctgcgaca tgatgtctgc cagataagcg taataagcag tgcagatcac
     1801 ttctgcttta gcaccgccgc ccagattcac ccagtcaccg aatccaagtt tttcgcggat
     1861 gaagtccttg ctggtttttt ccagaaacgc catgcccagc accatctggt cataatgttt
     1921 ttcaataacg cgcacatccc cgtaaaactg atacataagc cacgggcaga tcagcgccgc
     1981 atcgccccag gcaacggcac cgccctcatg aatcacatcg ggggcaacat gggcaaaact
     2041 gccgtccgcc tgctggctgt cctgaatcag atccaccagc catttggtaa agaatgcgcc
     2101 aatatccgcc gtatacagcg ctgtaggcat gaaaaactgg gcatcgcctg tccagcccag
     2161 acgttcatcc cgctgcggac agtccgtagg agcttccaga taattgcctt tcaaacccca
     2221 aacaatattg tgcgccaact gattgagcag cgggttggaa cttctgaaat ccgccacaat
     2281 atctacgtcc gcattgacta cctgcccggt caccgcttcc aagggcagtg cctgctcgat
     2341 gccgcgaatc tccacatagc ggaagccgtg gaaggtaaag gacggtttaa gaatttccct
     2401 cccctgaccg gacagataat agaaatcggt cgcctccgct ttgcgcaggg catcggtata
     2461 caacgtcccg ttctcctgca gcatctccgc atgacgaaca cggatcagct gcccggaacg
     2521 accttcaagt tctatgcgtg gccatcccac catgttctgc ccgaggtcat atacaaaaac
     2581 acccggacgg ggctcattgc aggattgtgc tttcaggcat tcaaaggctt tgaccggcgc
     2641 accgggataa gcctgcagct tctcagggac aacttccggc gcgtccatcg atactgccgc
     2701 ccaggcaccg gcatcataac cgggcatgtc ccagcccgac agtgccttcc ggaaatcacg
     2761 cacttctccc tgaagcagat cgctgccccg gatttctccg aaagccgcac gccagctgtc
     2821 atcagtcaca acgatctggc gattgccgtc ttcataatct atttccagct gagcataaag
     2881 agccggcttc gtcccgtaag ctgccgaccc tttcagaccg atatgtccgg cataccaacc
     2941 gtttgcaaga agggcaccca gcacattctc cccatcgggg cggatcaggc cggtgacatc
     3001 aaaacgctgg tagtaaagcc gttttttgta atctgtccag ccgggagtga agaagcagtt
     3061 tcctgccttc tctccgttga tccggaattc atacaagccc agagccgtcg cgtataaagt
     3121 cgcctctttg atgcccgcgc gcgtactgaa atccttgcgc agctgtgcca ccgggcggaa
     3181 ctgcgtcaga tcaggtgcag tgccttccca gggcgcctcg ttgtatccgg caatatcagt
     3241 gacaggctgc cagccttccg tatcagaagc acctgtcgtc caggaatccg ccggttcttc
     3301 cgtcttgcac aaccagctgc tgtctgtcgt aatatcgatc acattgccat cgcgcaatgt
     3361 aatctttaaa gcggcgataa gcccgctctg cgcttttccc gcagtcactg aaaacgccag
     3421 tacattctcc ccgggctgca gcagcgccgc aaccagatgc gggtacactt cgtgccagtc
     3481 cttcatttgc gggcgaccct gattcagctg ataaccgtta acccagccct gacaggaagc
     3541 ctcagccgtc acccacaatg atgcgttgac aataaccgca tcttccggca actgaaactg
     3601 ctttctgaaa taaatcgttt ttgcagattc tgcgttttca tcttccgacg gagcagtgcc
     3661 gatccagcgt gctgactcga tacgggcgac tgcggcggca tccagatctt ccgcaccaat
     3721 ggcattgaag ccgatccacc gggcatgcca gtcccgctca cacagcagac ccagactgaa
     3781 agaagccgta tcgctccagg cactcatgcg gttctgattg tcccacaccc gcactttcca
     3841 gaagcagcgc atccgggact gcagcggctc acccgcatat acaataaacg tagattctcc
     3901 ggaatccacc ttgccggaat cccagagcgt gcccctgtca tttgccaaat tttccggaga
     3961 atctgctacc agaatctgat aggcgtgctg caggcagttg cgctcctccg actgcagctg
     4021 ccagttgaag cgaggctgcc tctcttcaat gccttccgga ttgatgcgat attcagtacg
     4081 caattccaga acctgcaggt cagcggcggc tgatgcgcaa aaacccagcg atgccggccc
     4141 cagtacaatc atgctctctg aaaaaaccga catgactcat tctcctgttg ttggagccca
     4201 accagtactg cctgcccggg acaggccgaa tgcttctttc cccaggggag atcagtgccg
     4261 tgcgctgccg catcccttat gtttgctgca atgcaggcag gcatgatccg gatcatcaag
     4321 tacatcgcag tccggagcca gccctttccc gcgccggatt acatactgca cgccgagcca
     4381 gaacatgacc agcagaaaaa agatgccgcc gcccagcaac ccgttctgaa gtgcatgcaa
     4441 catgccggtc agcctccgag acctgcaaag cccatgaacg ccagcgacag aatgcctgcc
     4501 agaatcagcg tgatggcagc tccttgcgcc acatccggca catcggcaag ctccagcttt
     4561 tcacgcatgc ccgccatgat aatcaacgca agcgtatagc ccgcaccggc gccgacagca
     4621 taaacgatgc attggataaa gttgtattct ttctgcgtct gaaacatggc aagggcaagg
     4681 atggcgcaat tggtggtaat aagcggcagg aaaataccaa gcgcacggaa aagcgccggg
     4741 ctgtattttt taatgaccat ttcaaccagc tgcaccgccg atgcaatcac aacgatgtag
     4801 cagatcagtt tcagataagg cgcatcaacg ctgtcaagca caaagttgat gccatacgtg
     4861 cacacagaac agaccagcat gacaaaggtg acggcaatgc ccatgcgtac cgctgtgtcc
     4921 tttttgttgg acacacccag aaaggggcag atgcccagaa acatgctcag gacaaagttg
     4981 ttgacgatac aggcattaat aaagacggaa ggaagagatt caggattcat gatgctcgcc
     5041 tcttatgctt ttgcctgagc aagcgcccgg gcttccttgc gtttacgcca ccaattgaaa
     5101 aacagaagtt ccacaccgag gacgaaaaat cctccgggcg gcagtatcat gacagaccag
     5161 ggctcaaagc gcggaccgaa cagcgaaatc tcatagggcg tattcatcag aagcgtgccg
     5221 ttgccaagca cttcgcgcac aactcccagg cagagcagcg caatagtaaa gccgatgccc
     5281 atgcctatcg catccagcac ggaacgcata acaccgttgc gagaagcaaa tgcttccgcc
     5341 ctgccgagga taatgcagtt caccacaatc agctggataa acgcacccag actgtcataa
     5401 agatccagac tgatggactg aatcagataa tccacaatgg tcacaaaggt ggctatcacc
     5461 acaatgaagg aagctatgcg cacctgtttc ggaataaaat tgcgcagcat cgaaatcaga
     5521 atgcctgaca tgaccagcac gaacagggtg gacacgccca tggcaaagca gttgatcacg
     5581 ctgttagtca ccgccagtgt cggacaggtg cccagcagca tgacgaaaac aggattgtcc
     5641 cgccagaatc ctgcaaaaaa cacatcccac gaagaggggc cttttttctc catactcatt
     5701 tgccctgctc cttcaattcc tgcaagtgtt gatgaacaag agggagcacc acttctgtgc
     5761 tttctttcat gccattcacc acagctgtcg aggaaatcgt tgctcctgaa atcgcctcta
     5821 tctgccccgg tccgccgccc gctcctgcct tcaccagctt caacggatac agaagatgct
     5881 gtctggaatc atccagagga actgacagag actcaaagtt cttttgaaaa tgctcgtcgg
     5941 tttttatctt gtcgccaaga ccgggcgtct ccttgcacag gagcactttg gtgcccacta
     6001 ttttgtgctc atccggaata tagccgtaga gcagtttgat ttcacctcca taaccgccaa
     6061 gatctattgc ctccagcgca accccgagaa aagtacccga cgcatcatac cccgcataaa
     6121 cagtacgcgg aacatcaggg ctggcttctg aaaagaacgt aaattctttc tggatgaccg
     6181 aacccggaac cagttcaaga acggtgctcc ggatgcgttc ttccgtattc gtttgaatgc
     6241 gtgaaaaagt cgcctgatac actacagaca gcaggatacc cgacacaaaa gaaattgcgc
     6301 caagagtccg aagcattgcc agacttccgg cattgtcttt cttctgatcc acaggggggg
     6361 tggtttccga aacactcatt gcttgatcca tcttttctgt cgcaccagac cataagttct
     6421 gggttgggtt gcactggtaa tcaaagggga gacggcattg ccaagaagaa tggcaaacat
     6481 aacaccctca ggcagtgcac caaaaatacg gataatcact gtcaccatgc cgataaaaaa
     6541 accataaatc catacgcccg ccgatgtcat gggagatgcg accatgtccg acgccataaa
     6601 tagggcgcca aacatcaatc cgccggaaaa aagcatgaac atcggactgg gatagtgggt
     6661 tttatcggaa agccagaaag cgccgctcag aagaaaagca cccagaagca tgcccgcagg
     6721 aatgcgccag ttcatcatgt tccgggcaat caggtacaga ccgcacagaa tgatcagcca
     6781 ggtggcagtc tccccggtag atcccgctat ggaacccaga aagagccgtg gcagtgactc
     6841 aaactgaggc acttccgcaa acttccacat ggaaaggggt gtggcacccg accagccgtc
     6901 cacgccgccc gaggcgagtg ccgtgcgtat ataatccgca agtgccgcag tatcggcgcg
     6961 caggaaagga aaggtcagcg tggtcgggag caggctggaa aatctgccgg gaaggtcggg
     7021 cggtgtccag gtcgtcagcg ggatgggaaa ggcaatctgc aaaaaagcac gcccgaccag
     7081 tgcagggtta aagcagttgt agcccagtcc gccgaaaagc gatttcgtga cagaaacacc
     7141 aaaaatactg cccagcaccg ccatccataa cggcgttgac ggcggcaggg tcagaccgag
     7201 aatcagaccg gtcaccacca cactccaatc gcggatggtg gtggaacggc gtgcaatcag
     7261 gcaggcagta tgttctgtag ccacacatgc cgccgtagtc gtcagcagca gcagcagtgc
     7321 actgagtccg aaagaccaga ccgcaaaggc ggctacagga agcagcgcca gagccacatg
     7381 aaacattata tccggcacac tttgcttcac acggatatgc ggcgacgagc ctataatcag
     7441 cggacgggct tctgtgctca tttggcagcc tccctttccc ggttcattgc cttggcaata
     7501 cggaagtact gcacaagcgg aatattggac gggcacacat aggtgcagca tccgcattcg
     7561 aaacagtcat tcaggtgatg ctcctccgcc atctcctcat agcgcgcttt ccgcgccaga
     7621 agccccagca gagaaggatt cagaaaaatc gggcaggcat cgacgcagcg ggcgcatttg
     7681 atacagggat acaccgtacc gccgccgtcc cgcatctcct cttccgtcag cacaagcaga
     7741 cccgacatgc ctttggtcag cgggatatcc tgagaagaca gactcatgcc catcatggga
     7801 ccgccactga taatctttct tgcctccggg cggatcccca cctgttccat aatgtagcgg
     7861 ataggggtac ccagcggaat ccggtaattg cccggccgtt ccacaccgcc gcccgacacc
     7921 gtgatcacgc gttcaatcaa cccctgacct ttcggcagca gctgacccag ctgcagcagc
     7981 gtggcaacat taaaacaccc gacccctata tcgctcggaa gcccgccact gggcacatct
     8041 ttatgaagca ccgcccggct cagcatcttt tccgcaccct gcggatactt ggtcataagg
     8101 ctgcgcacac tgatgggcgc acccgccggg atagctttcg ccatgctttc cagggcttcg
     8161 ggcttgttat tttcaatagc gatgaacgcc tctttggcac ccatggcctc cataaccagc
     8221 tgaatgcctg taatgatctt atccgactcc tccaccatga tccggaagtc tgtcgtgaga
     8281 taaggctcgc actcgcagcc gttcacaatc agcgtgtcca cacttttgcc cgctggcggc
     8341 ttcattttca catgagtggg aaatgcggca ccgcccagcc cgactgtgcc cgtttcctgt
     8401 acggcacgga tcagctcatc cggcgtaaga gaagccaggt cgcggggcgt cccgtaaatt
     8461 tcttcctgat ccgcatcagg atctattttg attttgattg cctgcgccat ttcgccccgc
     8521 ggattcagag catagtccac tgccgtaact gtgcccgcaa taggcgcatg catgggcact
     8581 gaaacaaagc caaccgcttc ggcaatcggt tcaccccgca tcacttttga ttttttcgcc
     8641 acaagcgctt tggcaggtgc gccgatatgc tgagacagca gaatcgtcac ctccggcggc
     8701 agcggcaagg tctcgatcac tgcatccgcc gtatacttgc aatcgggcgg atgaacaccg
     8761 tggggaaaag aattgacata acttttcatg ctatcacccg gatacttctg atttttgttc
     8821 cgcttccgaa gctgacgttt ttttcaacgg cgcttcaggc ggattcagca ctttttccaa
     8881 agcagcggca gccttcacac ccagcacacg ccttccgtcc cgctcaaccc acaaaatcgc
     8941 tcccgacgga cagttttcga tggcttcacg aactgcaaat tcatttttgc cgtaatccac
     9001 aacaggcagg ttgccgtcca tcgataccgc ttccggcgcc gctttggcgc acagcccgca
     9061 gccgatacag gcaacggcac acgccgcctt ggcgtcttta ccccgaagac gggttgaaca
     9121 ggaaacccat aactgatggc tgataggatg gatggaaaaa agatgtttgg ggcattcaac
     9181 cacgcaattc ccgcaggcgg tgcatttcgc ctcatccacc acaggaagca tatgttcatt
     9241 catatgaagc gcatcaaagg ggcagaccgc tacacagtcg ccgtagccga tgcagccgaa
     9301 ggtgcagtgc ttgccgcccc ctgccaccag tgacgccgcc cggcagctgt agcggccttc
     9361 atagtgcgca aagcgacgcg ccacattatc accgccggaa caggcaagcc gggcaacccg
     9421 cttttctccg acacccgcat caactcccaa atacgccgct atgctctcta cggtatctgc
     9481 cgatgaagcc gtacactgac ccggcgccgc ttcgccccgg atcaccgctt cggcaaaagc
     9541 acgacacccc gcaaatccgc aggcaccgca gtttgctgat ggaagctgcg cttccacttc
     9601 atcaatgcgc gggtcttcat aaacatacag ttttttatta gctatcgcca gcactgccgc
     9661 aagcagcaaa cccagcaaaa gcattacgcc cccagcaata ctcaatgcag atatcagcat
     9721 ttgtcactcc tgattcgggc aaagatccga agatcctgat ccggtcgtag ttaaaatacc
     9781 gctcaccccg acgcagcaca cccggtactc aggcagcaac aaatactatg ccgtctgcac
     9841 gtaaagcagc agtgacatct ctgatggaag aagagaaatc cgcaggctaa aagcctgtct
     9901 gcatggcgtc tacttcccgg tcgatacgcg ggcgattcgt ccattcaccg ccccgttccc
     9961 tccagaaatt catcgccgtt ttcgcctgag gtgtcggcgc acgcatatca acaatggtgg
    10021 gcgacacaaa aacaagcaga ctggtaacct gtgcctcgga cttacggctt ctgaaaggaa
    10081 tgccgagcag cggcacgctg cccagaacag gaacgcgccg ctctgtcttg cggacagtac
    10141 ggctggtcaa accgcctacg accagggtgg tgccgtcggg aacaaacacc actcccgatt
    10201 gtgatcgggt ggaaaacacg ggaagatcgg cgccgcggag attctcatag ctgaggatat
    10261 ccgagacttc cagttgggtg atggcaagct ggacaaaatt gttgggcata atcgttggcg
    10321 tcaaattcag attaacgccg atgtcacgcc aggaaacctg cagacgcgta gctgccgacg
    10381 gaaaagtcac gtcctgaaag ggcacctgac cccctgcctt aatcgtggca ggaagcgtgt
    10441 tggcaaccat aatttccggc ttggaaatca gatccagatc actgcgtgtc tcaagggact
    10501 gaaaaatgcc gtctatggtg ccgtaatccg catccagaat actgaattcc agacccagcc
    10561 cggggtaggc ttccaactgt gtcgtagcag ggttcaggtc aggacgcaga ggcgctccga
    10621 acagtgcttg atgaggataa ggcatcgcaa ccgtaccgaa agtcgacaga ggattgatgg
    10681 tggaagtggt tacctgctgg acagagccgg tcatttcctg gccccgcaca aaacgggtaa
    10741 agtcgagatt ggcgcccaga tcacggacgc cgccttcgct tgtttcgctg atccagactt
    10801 tcatctgaac ctggcgcaca tcggcaggag gagcagcagc aggagccgcc tcagcaggcg
    10861 ccgcttcagc aggagcggcg gcaggcgctg catcatccgc aaaagaagac agcgcaagaa
    10921 aaaccagggc gagaccggca agcgcaaagg gcaaagagaa aagcctgttc attattcacg
    10981 ccctccaaga ctgtcgctca cgccgctcgg cacggtattt gatcccccga aaagactgcc
    11041 gataaagccg gacggaggcg taggatgcac aatatcttcc tctccttcct cttccgatgc
    11101 cgcatcttct cccccgggag cgccgaaacc ctcgtacaaa gtaaaagcct gacgccagat
    11161 atccgctttc accataaaga ccagttcgcg ccgggtttca gaagaacgct tattgcccag
    11221 agcggaagtc agcggaatgt cgggtgtctc cgaaaagggt gaaaggcgct ccagcgtgtt
    11281 gttcaccaga tcttccccct gtgtcagcca gggaagggtc gaaacgtttt tattcttcgt
    11341 attgcggtac agtccgccaa ggatcagcac ctgattgttt ttgacccaga ctgtcgtatc
    11401 cagactgcgc gacacaaact caggcacgct gatggcattg gtcgaacggg acacgacagc
    11461 accggtactc gcaaaagtat cggcaagagc aatggtaatc cgttgacctt cttcattaat
    11521 ttccgccgta agtttcagct ggatatactg atcctccgtt gtattgggat tgccgtcatc
    11581 atccaccacc tgaagcgcac tgaccgtgag ggtcacgccg gtaggacgga acgctgtcgt
    11641 ctgtactgca ttcaccccca caaccaccgt gttttcgtag ggtacatcct gcgtggtttt
    11701 tataatggtg ggggcggctg cacctacgga aaccataact ttcggctgcg aaagaataaa
    11761 ggcgcggttc tgatccacca gcgcctgcag aatcatttct atatcaccgt aataggtgct
    11821 gatctgatca agaaaaaggg tgatgcccgc cgtggatcga ttgggaaatg ccagcgatgc
    11881 agcatcaata atgcccgggc ccgtggagac aagcccgtag ggacgcgggg taatccgttt
    11941 cttgaaatag gcgctgattc cgctttctgc gcctttcgtc gtctgaaatt caatgatctt
    12001 taccgataca ttgacttgct gcggatcctg cccctgtgca aagccgcaca atagcagcgc
    12061 agccaggatg tatacagatc tttttatcat tcctttggat ccagttcagc gccggtaact
    12121 gcccacacat tcatggcgcg ttatattgtg tatatcgtca aatcgtgaaa attgagcatt
    12181 gaatgcccga atctcacggg ggcgcaaaac gccgagcgat atcgtttgcg tatcatagac
    12241 catttgaccg aaaccataaa tcgtaaccgt tatgcgaaca tcttccagcg gcgcttcgct
    12301 ctggttcacc gcctggccgg ctacattata aaacttctca cgggcggtcg tgggccagcc
    12361 ctcaaaccga ccgctccgga aggaggacac attatatata agtaagggct ccgcttcctg
    12421 atagcgacgc tccatctccg ccggggttag aataacaagc cggccggacg catcgcgttc
    12481 tacacgacgg ctcaccgctg catcggaggc aagctgaggc caccgcttca gcagctcgtc
    12541 ataggccttg ccagcctgat catagcgacc tttttcccac agcgtattgg catagtccag
    12601 acggggtgcc atcgcttcag gatctgccgc aatcgccgct tccagagccg caaaagcatc
    12661 atcgcctgct gcctttccgc tttctccgcc acccgcaagc ttttcaagca gctggcggcc
    12721 ttcggcgctg ttttcgccct ccggaaccag tacaagatat tcctgcagcc agcggtgcgc
    12781 atcggaatag gcgcctactg tataaaaagc tttacccaga aaaaagcgcg cctcaatggc
    12841 ggacgcatcg ccgggatatg tgttgacaag cgtttgcagc cgagggataa cgagcgacga
    12901 ctcgcctgct ttgatcatgc gccgcgctgc tgcaagttct ttggcggcac tccctgaatt
    12961 gctttcgggc gcacgggcta taacatcgcc gacctggaca ggccgcgaag cctgctgccc
    13021 gctggcggag cccggagttg cgcaaccggc aacaaccgaa aagagcggaa gcagccataa
    13081 tactgccggg aaaaccagaa cagcagcgcg gaaacactgg ttaatagatt gcttcatgca
    13141 cccggtctaa gtcctatttg gaaagttttg ctagtgccag ttctgtgata tcttccgtcg
    13201 caatgggcgg cgtcaatcct tcaaggtatc ccttgagaac aatcagatca aagtccgttt
    13261 cactgccgac actgctgatc gcacggatgg cgcggtcatt ggctttgctg ataaaaagcc
    13321 agtactgggc ggtgtttttt tcagctttta ccgcctcttt atactcggtc gtagctgaaa
    13381 ccaccgcatt gtaatccacc gatccgggct tctcaaaact gcttgcagtg ccccagaaga
    13441 tccgctgatc atcaaccgct tctggaggaa tagacattga ttctgccata gcaggtgcgc
    13501 tgaaagcaaa aaaatacagt gaaatcaaca ggattatcag aatttcttca aacctgttct
    13561 tattctcaat catcattagt ccttgcagaa acagtcccga agccgctgcc ccgacttctt
    13621 tcttagcggt tccggaccgg actgcatcac ttatgtcacc ttatcttaca ggtcagtata
    13681 ccatagcgtc acagcattct gcccgacacc atctgtaaaa acaggcacaa gtacctttct
    13741 ctctctaata cctcgcccgg acggaaagtt cccggaaagc ccggagtttt aaaaaagcgg
    13801 cactatacat agaagattta ttactattat acactaaata ttgtggttcg tgccgccgta
    13861 taaaaagcag gcctgcctgt agcgactgcc ggaaagcaaa agttccggat ccctctaacc
    13921 ccttctgcat cgcttttgga gcagtcccgg aagcagagag acgctttttt aaccgtacct
    13981 aaaataaaac agccggcttg
//
LOCUS       DAOPWB010000082        13775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4537_length_13775_cov_6.112245, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000082 DAOPWB010000000
VERSION     DAOPWB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4537_length_13775_cov_6.112245"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..304)
                     /locus_tag="PKN92_08600"
     CDS             complement(41..304)
                     /locus_tag="PKN92_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48891.1"
                     /translation="MQTSVHITKTFFFAGLMVLCFTLFQMTGFGDNYSGSSFGNDIKI
                     NKDLEKAPEKENADEQYNKGECYYSGDGMIEDKRCAVEWDNVP"
     gene            696..2615
                     /gene="dxs"
                     /locus_tag="PKN92_08605"
     CDS             696..2615
                     /gene="dxs"
                     /locus_tag="PKN92_08605"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNZ48892.1"
                     /translation="MEPPILDSLHGPGDLKQLAPEQLDALASELRQRILHTVNLNGGH
                     LASPLGVVELTIALHYCFNAPEDKIIWDVGHQCYAHKLLTGRADRFSALRKKDGISGY
                     PKMCESVYDAFGTGHSSTSISAALGMAMARDRLGLDHHIAAVIGDGAMTGGMAFEALN
                     HVGHLKPKMLVILNDNEMSIARNVGAMAHYFNRMITNRPYKRAKQDVGSFVKSIIGDR
                     MVRTIQDLEKSVKGFITKGGLFQELGLNYVGPVDGHDLPLLIELLNRIKDMDGPVFLH
                     CTTEKGKGLKAAEENPQKYHGVNPMILDTDKGEGDALPAQKTAESVPAVPSKSFTDAF
                     ADALVSLGAEDSRIVAITAAMPTGTGLSRFEEHYPDRFFDVGICEQHAVTFAAGLAVE
                     GLRPVVAIYSTFLQRGYDQLIHDVCLQKLPVVFAVDRAGLVGEDSPTQNGIFDLSFLR
                     AVPDLRIAAPRDARDTALLLEWAMTQEMPVALRYARSSAPLIGKEDGRAVDRAERLRD
                     GQDLVFLTVGPCASQALKAAELLANEGIGAAVIDARHIKPLDGAMLDSIAARPIITVE
                     ENTLPGGFGSAVLEYFEGRSTQAPVRIKRIGLDDIFGEQATRAEQINMYGLDGASLAA
                     HARAFLAAGSPAPDA"
     gene            2628..2834
                     /gene="thiS"
                     /locus_tag="PKN92_08610"
     CDS             2628..2834
                     /gene="thiS"
                     /locus_tag="PKN92_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNZ48893.1"
                     /translation="MSITLTVNGESKSVDGGTTLASLVEELGLTIDSLVALVNEEVVE
                     RTQFADTALSEGDQVELVTFLPGG"
     gene            2834..3490
                     /gene="thiE"
                     /locus_tag="PKN92_08615"
     CDS             2834..3490
                     /gene="thiE"
                     /locus_tag="PKN92_08615"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNZ48894.1"
                     /translation="MMQVADRIVRFREADLYVVITEAYCAGRSGLDVLDACLEAGVRL
                     VQLREKHMDDRQLYETARLWRKRTKAAGALFILDDRVDIALASGADGVHLGQNDLPFP
                     AARALAPDLILGLSTHNPEEALAAQEAGASYINIGPIFPTNTKEVPTGAVGTAMIEAI
                     RPLLHIPFTCMGGIKANNIDQVLSAGASIAAVVTAVTAAPDVSAAAAELRKKILQYRA
                     "
     gene            3720..9956
                     /locus_tag="PKN92_08620"
     CDS             3720..9956
                     /locus_tag="PKN92_08620"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HNZ48895.1"
                     /translation="MFKKITVALFLVVCLSVFLFPLTVYAYSGGAGTAINPYLLSSEM
                     DWHELCDHSQDWSKHFKLTQDIDFSGTVAPVGNKNTPFTGTFDGNNYRCWNASTDTGT
                     ELGALFGIIDGDAVIRNLGTVFGVMHGTAAAGGLVAEMRGGTVQDCYARNEVKPASSL
                     PAGGLVGVMSGGTIINCYSTGDVTRLNQAGGLIGVRSDGTVVNSYWDIETSGQATSAG
                     GKGLTTAEMTYPYGSFVYAGWSRGWHSDAGTVNNHYPYRSNNAHQYSEGDGSAGSPFV
                     IASSWDWELFSSSYNLTNKNFILAADIDLAGMALTPVGAGTGGITGLFFDGQGYSIRN
                     ATIHGGDYAGLFSRADSSVLKNVCVNTISVEGGDYTGGLAGSASSSLIENCSVTGHVE
                     GGNYTGGFIGTCSSQINHCFSRASVTGINNVAGFAARITGDDGVVTYSYSAGAVSAVG
                     TDIFGFSPYSDWVGACYWDMESTGVNSSSGGGIGRTTAEMTTPYAANTYEFWDFSSGG
                     PWTDDFLMLRNDGYPLPRTLLHFSKKYSGGDGTAFTPYQISTPGDWVQLMITQEDWEG
                     KHFELTADLDFQGYDLHCVGKEGIAFTGVFDGQYHKVSKGRIYFPENSYVGLFGNVDG
                     DGATTGILRNLTIEDFSVTGGEQVGILAGYAKGAIQRCEASGEVAGDGWVGGVCGQFQ
                     GTLSYSSFRGSVAADYTRAGGLCGVINVSSVLSCRIDAVVQGTDYTGGLAGRAFHTDI
                     EKCHVSGTVSGSDYSGGLAGLFTVSGSTIKAVSESSSSATVTGAVYTGGLFGQASNLS
                     LINAYASGAVTGVREVGGLGGRFVNCQSFRCYACGAVSGDDATGGLVAVNDGGADPGS
                     YWDVDASGQYFSGRGTGKSTADLTWPYANPSIWQVWDFDNIWFHDISGKKNGGYPLLR
                     DRLFEANPGYAGGSGTEEDPFLIDNAGDLQVLAETPLHWGSHFRLTADIDFQGQNIAG
                     IGALTPVQLAFTGTFDGAHYSISNGNINEAGEAGGLFKVVQYPGVIRNLHLFDFSVSG
                     ENNVGSLAGKLSGVLVENCSVSASVRGVNTVGGLIGLAEDDSRISTCWAVVDVKGKTN
                     TGGLIGRMTDSTAEYTDLEGTVESSDSMGGGFVGRGTNSVFMNNGASADVAGKKYIGG
                     FAGLLTGGETDQCSASGEVTGQGDYPDSLGGFAGSLESEAFIHNSTASGNVKGNASYS
                     GGFVGYGTIQLLVEECSAEGSVEGTENTGGFAGYLIEDCRIKTCSAAGNVSGRKSAGG
                     FVGLALEDTSFDQCTSTGDVEASGDHTGGFAGYLYETELSDCSSTGAVTGNNFVGGFL
                     GKIASTSSTLAECTSTGDVEGREHVGGFAGSLVDTPATACHASGSVEGRKHTGGFAGF
                     IKNTTVQTCYAEGLVKGVGSNPEATGGFAGTVEYSSKLQSCYASGKVTGTSDVGGFVG
                     DAVGYYEGYLDMEACYALGTVKGENNVGGFGGSLRESLTVKKSHAGGTVEGLENTGGF
                     AGWVFEGGEISYNHALGTVEGKTATGGFVGAVSAGSNFRYCFTLGNVEGTTDTGGFAG
                     KMHQAEVECCYTRGAVKGKSSTGGFAAKSLQTALLDSCYAAGAVSGDTGAGGFVKSAD
                     GPVTRCYWDMDLAGLSWSAAGEGLSSADMRFPASSSHFVGWDFDTIWRVDYADENDGY
                     PLLREEGEQYSGGTGTRENPYQLLLPADWAKFSASPADWSKHFALTADLDFAGQTFTP
                     AGNSITAFTGSFDGLDHAIRNVVLTADGDGTQGDSRAIFGVVGSGGAIRNLHCTSIEI
                     SGRDRVGGLVGAMNKGTVAGCTLTDSSISGRSHVGGLVGYAENHSRIENSATDSTVRA
                     SSDRAGGIAGSGEGSIFRRVQSGGIVSSAGSMAGGLSGFADASEFQDACSTASVSAVD
                     RAGGLLGEASYSNIERCYAIGMVEPSGKTGALTGGLIGFDAGGSEIMASYWNQESTGQ
                     GGSVSAGRRNTDQMAGESAQSNYKNWDFDGVWAMPEDLRLNQGYPYLRAIPAAVAADS
                     WHPADLNRNWTLEENEALHYMERWQKGAVPMVWAVRTGFLSRAGHSYVFDASKPMPDC
                     WSRP"
     gene            10582..11961
                     /locus_tag="PKN92_08625"
     CDS             10582..11961
                     /locus_tag="PKN92_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNZ48896.1"
                     /translation="MGDHKIRKGLTLPIKGEPSRVVAEKKVSRIAVMATDFVGLKPRM
                     LVQPGDTVQRGQALFEDKKTPGTFFTAPGAGTVSAVNRGEKRMLQSVVIDLSESESTG
                     NPSPDELKSFSSYTGKSAADLSGQELRDLLTEAGMWPAFRTRPFSGRPLIDSEPHSIF
                     VTAMDSSPLAPPMNMVVEGREAALAAGLTALTKLTGGTIWLCVAPDAGFIPSDLPASV
                     KVERFSGPHPSGTAGVHIHTLDPVGRNKTVWHIGLQDLLAMGDLIRTGVLCVDRLISL
                     AGPGVKEPRLIRTRLGACTDCLTAEELIEGEARVISGSVFSGRKSMGDVFGYLGRFHN
                     QISVLAEDREKVFMGWLTPGADKFSLSRLFLSKLFPSKRFSLTTSTNGSPRAIVPVGL
                     YEQVMPMDIIPTLLLRSLCVLDVDQAEKLGCLELDEEDLALCSFVDPGKEDFGSLLRK
                     NLDIIEKEG"
     gene            11970..13235
                     /locus_tag="PKN92_08630"
     CDS             11970..13235
                     /locus_tag="PKN92_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HNZ48897.1"
                     /translation="MKFIRTLFDKQAPLFEKGGKLEVFYPLFEAIESFHFTPGKVTDG
                     APHVRDAVDLKRIMITVVVSLMPAIFLGLWNIGYQINSGLAPGVTPEGWRAGLVAALG
                     LGFSPASFLACFVHGALYFLPVLIVTFMVGGSIEVAVAIIRKHDVNEGFFVTGFLIPL
                     ILPPTIPLWQVALATAFGVVIGKEVFGGTGMNIFNPALVTRAFLYFAYPIQSSGDKVW
                     VALNPAQAADGFSGATLLARVAEIPPVADGQAWQTAVSTMQINGKAVSWLDAFIGWIP
                     GSMGETSTLACLVGAAILLITGVASWRIMVSMVAGALGLVLLMNAAGSQTNELMNLPF
                     HWHFVLGGFAFGLVFMATEPVTGTYTETGKYIYGFGIGVMAILIRALNPAYPEGVMLA
                     ILFMNILAPLIDYAVVSQNIKRRAARSAI"
     gene            13237..>13775
                     /gene="nqrC"
                     /locus_tag="PKN92_08635"
     CDS             13237..>13775
                     /gene="nqrC"
                     /locus_tag="PKN92_08635"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HNZ48898.1"
                     /translation="MKYIFGFAAALCLICSVLISVANVGLRDRQEINKQLDKKKSVLQ
                     AACLIQAGEKTNRARIESLFENIRARVIDLSAGRVLDDVDSARFNEEDIPEQPAPANK
                     AGIRTLRNQMLIYEVMDGDRISMFVLPIVGKGLWSTMYGFLALDADTKTIRGLTYYDQ
                     GETPGLGGEVTNPKWTALWP"
BASE COUNT         2980 a         3408 c         4067 g         3320 t
ORIGIN      
        1 accaaacgat gaccccgttt gtccaaataa tttacagaaa ctatggtacg ttatcccatt
       61 caacggcgca ccttttatct tctatcatcc catcgccaga gtagtaacac tctcctttat
      121 tatattgttc atcagcattc tccttttcgg gtgctttttc cagatcttta ttaattttta
      181 tatcattacc gaaactactg ccgctgtagt tatcgccaaa acccgtcatt tgaaacagag
      241 tgaaacataa aaccatgagc cctgcaaaga aaaatgtttt tgtgatgtga acgctggttt
      301 gcatgtcctg ctccttatgc attgtgatac gttttgacaa tcaacactgt gctgccctct
      361 gaggctgagc agtacgcttt cagtcctcag caatttctcc ctgtataatc tgcctttcat
      421 actattcatt tctgtttaca caattcaatc ttgctatttt ctggagaaaa tgcgggtgcg
      481 aaaagatctt tccttcctct tctttacagg cttcaggcgc gtatcagaca ttcagggggc
      541 agggagtggt atactcttag acacaggaca agttattcta tctgatgcgc aatccttccg
      601 gagttccttt tcgggtcagg atcgctttac caatcagccg atgccgcttt tttatgcagc
      661 ctttcgctgt ctttgccggc aggatctcaa gaatgatgga accccccatt ttagacagcc
      721 ttcacggtcc cggtgacctg aaacaattgg cgcctgaaca gctggatgcg cttgcgtcgg
      781 agctgcgcca gcgcattctg catacagtca atctcaatgg cgggcatctt gcttcgccgc
      841 tgggcgtggt ggagctgacc attgcgctgc attactgttt taatgcgccg gaagacaaga
      901 ttatctggga tgtgggccac cagtgttatg cccacaaact gcttacaggc cgtgctgacc
      961 gtttttccgc cttgcgcaaa aaagacggga tcagcgggta cccgaaaatg tgcgaaagcg
     1021 tatacgatgc tttcggcacg gggcacagct caacttccat ctcggcggcg ctgggcatgg
     1081 cgatggcgcg ggaccggctc gggctggatc atcatattgc ggcagtgatc ggcgacggtg
     1141 ccatgacggg cggcatggct tttgaagcgc tcaaccatgt gggccacctg aagccgaaaa
     1201 tgctggtcat tctgaacgac aacgagatgt ccatcgcacg caatgtgggc gccatggcac
     1261 attatttcaa caggatgatt acgaaccgcc cctacaagcg cgcaaaacag gacgtgggca
     1321 gttttgtcaa aagcatcatc ggcgaccgca tggtgcgcac cattcaggat ctggaaaaat
     1381 cagtgaaggg ttttatcacc aaaggcggtc ttttccagga gctgggcctg aactatgtgg
     1441 gcccggtgga cggccatgac ctgccgctgc tgattgaact gctgaaccgt ataaaagaca
     1501 tggacggtcc tgtatttctg cactgcacga cggaaaaagg caagggcctg aaagcggcag
     1561 aagaaaatcc gcagaaatac cacggggtga accccatgat tctggacacg gataaaggag
     1621 aaggtgatgc gctgccggcg cagaagacag cggaaagtgt accggcggtc ccgtcaaaaa
     1681 gcttcaccga tgcttttgcc gatgcgctgg tgtcgctcgg cgccgaagat tcccgcattg
     1741 tcgccatcac cgccgccatg cccaccggta ccgggctgtc cagattcgaa gagcattatc
     1801 ccgaccgctt ttttgatgtg ggtatctgtg aacagcatgc cgtcaccttc gccgccggtc
     1861 ttgcggtgga agggctgcgc cctgtagtcg ccatttattc gacttttctg cagcggggct
     1921 acgatcagct gattcatgat gtatgcctgc agaagcttcc tgttgtcttt gctgttgacc
     1981 gcgcggggct tgtgggcgaa gacagtccga cacagaacgg catttttgat ctgtcttttc
     2041 tgcgtgcggt cccggacttg cgtattgcag cgccccgcga tgcccgggac acggcactgc
     2101 tgctggaatg ggcgatgaca caggaaatgc ccgtggcact ccggtatgca cggagcagcg
     2161 ccccgctgat cggcaaagaa gacgggagag cggttgaccg cgcggaacgg ctccgtgacg
     2221 gacaggacct tgtatttctt acggtgggac cctgtgcgtc acaggccttg aaagctgcgg
     2281 agcttctcgc taatgaagga atcggtgcgg cggtgatcga tgcccggcat atcaagccgc
     2341 tcgatggcgc catgctcgac agtatcgccg ccagaccgat tattacggtg gaagagaata
     2401 cgcttccggg cggtttcggc agtgcggtgc tggaatattt tgaagggcgc agtacgcagg
     2461 cacccgtacg gattaaacgt atcggtctgg atgatatctt cggtgaacag gcaacccgtg
     2521 ccgagcagat caacatgtac gggctggatg gtgcgtcact ggcggcgcat gcccgcgctt
     2581 ttctggcggc gggttccccg gcgccggatg catgaaagga ggctgttatg agcatcacgc
     2641 tgacggtgaa cggtgaaagc aaaagtgtcg acggcggaac cacactggct tcgctggtcg
     2701 aggagctggg acttacaatc gattcgctgg tcgctctggt gaatgaagaa gtcgtggaac
     2761 gaacacagtt cgccgacacg gctttgtccg agggcgatca ggtggaactg gtgacctttc
     2821 tacccggagg ctgatgatgc aggtagcaga cagaatagtg cgttttcgcg aagcagacct
     2881 ctatgtagtc attacagagg cgtattgtgc aggtcgttcc gggctggacg tgcttgacgc
     2941 ctgtctggaa gcgggtgtgc ggctggtaca gctccgtgaa aagcatatgg atgacaggca
     3001 gctgtatgag acagcgcggc tctggcggaa gcggacaaaa gcggcagggg cgctgttcat
     3061 actggatgac cgggtcgata ttgctttggc ttccggtgcc gacggtgtgc atctgggtca
     3121 gaatgacctt ccctttccgg cggcgcgtgc cctggcacct gatctgattc tggggctgtc
     3181 cacccataat cccgaagaag cgctggcagc gcaggaggca ggcgccagtt atatcaacat
     3241 aggcccgatt tttcccacca atacaaaaga ggtgcccacc ggcgctgtag gcactgccat
     3301 gattgaagcc atccggcctc tgctgcatat cccttttacc tgcatgggcg gcatcaaagc
     3361 aaacaatatt gatcaggtac tttcagcggg cgcttccatt gccgccgtgg tcactgcagt
     3421 gacagcagcg ccggatgtat cggcagcagc ggccgagctt cgcaaaaaaa tcctgcagta
     3481 ccgcgcctga gcggtttttt cctgtttttt atccctcctt ttttcttctc cttcccgaac
     3541 tatttctaaa atatgtgata caattactga tgtgtatttg catatattgc agatatgtga
     3601 gtagtattag gaaatgaaca tatcccgtat ttagtagttt gttgttacag catattttct
     3661 gattctgacg ggcgcaacgt ttctcttatc cgtccgacgt aaccattctg gagtacttaa
     3721 tgtttaaaaa gattactgta gccctgtttc tggtggtttg tctgtctgtg ttcctttttc
     3781 ctttaacggt gtatgcctat tcaggcggtg ccggcacagc tataaatcct tatcttctgt
     3841 cgagtgaaat ggactggcat gaactgtgcg atcactctca ggactggagc aagcacttca
     3901 aactgactca ggatatcgat ttctctggca cggttgcacc tgtaggcaat aaaaacactc
     3961 cttttacagg cacttttgac ggtaataatt atcgctgctg gaatgcgtcg acagataccg
     4021 gtacagagct gggtgctcta ttcggcatca tcgacggcga tgctgtgatc aggaacctgg
     4081 gaactgtctt cggcgtaatg catgggacag cggcagccgg cggcctggtg gcggaaatgc
     4141 ggggcggcac ggtacaggat tgctatgccc gcaatgaggt gaaacctgcg tccagccttc
     4201 cggcaggcgg tctggtcggc gtaatgtcag gcggtaccat cataaactgt tattccaccg
     4261 gagatgtgac acggctgaat caggcgggcg gtctgatcgg agtccgctca gacggcacag
     4321 tggtcaattc ctattgggat atcgagacga gcggtcaggc aacgagtgca ggcgggaaag
     4381 ggctgacgac agcggaaatg acctatccct acggctcctt tgtctatgcg ggctggagca
     4441 gaggctggca ctcggatgct ggcacggtaa acaaccatta tccttaccgg agcaacaatg
     4501 cgcatcagta cagtgagggt gacggcagtg ccggatcgcc ttttgtgatt gcgagttcct
     4561 gggattggga gctgttcagt tcgtcttaca atttaacaaa caaaaatttt atacttgctg
     4621 cggacattga tcttgccggt atggccctca cgcccgtggg agcgggtacg ggcggtatta
     4681 cagggctctt tttcgacggt cagggatatt ctatccgtaa tgcgacgatt cacgggggtg
     4741 attatgccgg gctgttcagc cgggcggatt ccagtgtgct taaaaatgtc tgtgtgaaca
     4801 ctatttctgt tgaaggcggt gattatacag gcggtctggc gggcagcgcg tcaagttccc
     4861 tcattgagaa ctgctctgtt accggacatg ttgaaggcgg caattataca ggcggtttta
     4921 tcggcacctg cagcagtcaa attaaccact gtttttcccg tgcatctgtg acgggcatca
     4981 ataacgtggc aggttttgcc gcccggatta caggcgatga tggcgttgtg acctacagtt
     5041 acagtgccgg cgctgtatca gcggttggca cggatatatt cggcttttct ccttactcag
     5101 actgggtcgg cgcatgctac tgggatatgg aaagtaccgg agtaaacagc agttccggcg
     5161 gcggcatcgg gcgcacgact gccgaaatga caacacccta tgcggccaat acttatgagt
     5221 tctgggattt ttccagcggc gggccgtgga cggatgactt tctgatgctg agaaatgacg
     5281 gttatccgct tccgcgcacc cttcttcact tcagtaaaaa atacagcggc ggcgatggca
     5341 cggcctttac tccctatcag atttctactc ccggcgactg ggtgcaactg atgatcacgc
     5401 aggaagactg ggaaggaaag cattttgaac tgacagcgga tcttgatttt cagggctatg
     5461 atctgcactg tgtcggcaag gaaggcattg ctttcaccgg cgtatttgac gggcagtatc
     5521 ataaagtcag caagggccgt atttattttc cggaaaattc ctatgtcggg ctttttggca
     5581 atgtggacgg tgacggcgcc actaccggta tcctgagaaa tctgactatt gaagattttt
     5641 ctgtgacggg cggcgaacag gtgggcattc tggcaggcta tgcgaaagga gccattcagc
     5701 gttgtgaagc cagcggtgag gtagcgggcg acggatgggt tggtggtgta tgcggtcaat
     5761 ttcagggtac gctttcctac agcagcttcc ggggaagtgt tgccgctgat tacacaagag
     5821 ccggcggact ttgcggggtg atcaatgtaa gcagtgtgct gtcctgccgg attgatgccg
     5881 ttgttcaggg cactgattat acgggcggtc tggcaggaag ggcatttcat actgacatag
     5941 aaaaatgcca tgtgagcgga acggtgagcg gttcagacta cagcggcggc ctcgcgggtt
     6001 tgttcaccgt gtccggcagt actataaagg cggtgtccga atcatcttct tccgccacgg
     6061 taacaggtgc tgtatataca ggcggccttt tcggtcaggc gagcaacctg agtctgatca
     6121 atgcctatgc ctccggtgct gttaccggag tcagggaagt gggcggtctg ggaggaagat
     6181 ttgttaactg ccagtccttc cgctgttacg cctgcggcgc ggtctcggga gatgatgcaa
     6241 ccggcgggct tgttgccgta aatgacggcg gagccgatcc cggttcctac tgggatgtgg
     6301 atgcaagcgg tcaatatttc agcggcaggg gtacagggaa gagcaccgcc gatttgacct
     6361 ggccctatgc gaatccgtcg atctggcaag tttgggactt tgacaacatc tggttccatg
     6421 atatatccgg caaaaaaaat ggcggttatc ctcttttgcg cgacaggctt tttgaggcga
     6481 atccgggata tgcgggcggc agcggcactg aagaagatcc ttttctcatt gataatgcgg
     6541 gcgacctgca ggtgctcgcc gaaacgcccc ttcactgggg ctctcatttc aggctgaccg
     6601 ccgatattga ttttcagggg cagaatatcg ccggaatcgg tgcactaaca cctgttcagc
     6661 tcgcatttac gggaactttc gacggtgcac actacagtat ttccaacggc aatataaatg
     6721 aagcaggcga agccggcggg cttttcaaag tggtacagta ccccggtgtt attcgcaatc
     6781 ttcatctgtt tgatttcagt gtttccggcg aaaacaatgt gggctccctt gcgggcaaac
     6841 tttccggagt ccttgtggag aactgttccg tgtccgcctc tgtccggggc gtgaatacgg
     6901 tgggcgggct gatcggcctt gcagaagacg acagccggat ttctacctgc tgggctgtgg
     6961 tggatgtcaa aggcaagaca aacacaggcg ggctgatcgg caggatgacg gacagcactg
     7021 ctgaatacac cgatctggaa ggtactgttg aaagcagcga tagcatgggc ggcgggtttg
     7081 tcgggcgagg aacaaacagc gtgtttatga acaatggtgc ttctgcggat gttgcgggga
     7141 aaaaatatat cggcgggttt gccggtttgc ttacaggagg cgaaacggat cagtgttcag
     7201 ccagcggaga agtgacaggg caaggcgact atccggacag tctgggaggt tttgccggaa
     7261 gcctggaaag tgaagcgttt attcacaaca gtactgcttc gggaaatgtc aaaggcaatg
     7321 caagttactc cggtggtttt gtcggatatg gtacgataca gcttctcgta gaggagtgct
     7381 ctgctgaagg ttcggtggaa ggcacagaga atacaggcgg gtttgcgggc tatctcattg
     7441 aagattgccg cataaaaact tgttctgcag caggtaatgt cagcggcaga aagagcgcgg
     7501 gcgggtttgt cggcttagcc ctggaagaca cctcttttga ccagtgcact tccacaggtg
     7561 acgtcgaggc cagcggagac catacgggcg gttttgccgg atatctgtat gagacagagc
     7621 tcagtgattg ttccagcact ggagccgtga cgggaaacaa ttttgtgggc ggtttcctgg
     7681 gcaaaattgc cagcacatcg agtaccctcg cagagtgcac ttccacaggc gacgtggagg
     7741 gtcgggagca tgtgggcggc tttgccggct ctctggtaga tacccccgcc acagcatgtc
     7801 atgcttcggg cagcgtggaa ggccggaagc acacaggcgg ctttgccgga ttcattaaga
     7861 atacaactgt acagacctgt tatgccgaag ggctggtgaa aggcgtggga agcaatcctg
     7921 aggcaaccgg cggttttgcc ggtacagtgg aatatagcag taaactgcag tcctgctatg
     7981 cttccggcaa ggtgacaggc acttctgatg taggcggttt cgtgggggat gccgtgggct
     8041 actacgaagg ctatctggac atggaggcat gctatgctct gggaactgta aagggcgaaa
     8101 acaacgtggg cggtttcggc ggaagcctcc gcgaaagcct cactgtgaag aaaagccatg
     8161 ccggagggac tgtggaaggc ctggagaaca ccggcggttt tgcgggatgg gtatttgaag
     8221 gcggcgagat cagctataac catgcgctgg gaactgtaga gggaaaaact gcgaccggcg
     8281 gttttgttgg cgctgtgagt gcgggaagca acttccggta ttgctttact ctgggcaatg
     8341 tggaaggtac aaccgataca ggcggttttg ccggcaagat gcatcaggca gaagtggaat
     8401 gctgttatac ccgcggcgct gtaaaaggga aaagtagtac gggcggtttt gcggcaaaga
     8461 gcctgcagac agctctgctg gacagctgtt atgccgccgg agcggtgagt ggtgatacgg
     8521 gtgcaggcgg ctttgtgaaa tccgccgacg gccctgtgac ccgctgctac tgggatatgg
     8581 atctggcagg tctttcctgg agtgcagccg gtgaaggact ttccagtgcg gacatgcgtt
     8641 ttccggcaag ctcctctcat tttgtcggct gggattttga cacaatctgg cgtgtggatt
     8701 atgcagatga aaacgacggc tacccgcttc tgcgtgaaga aggggagcaa tacagcggcg
     8761 gcacaggcac cagagagaat ccttaccaac tgctgctgcc tgcggactgg gcaaagttct
     8821 cggcgagccc tgccgattgg agcaaacatt ttgcgctgac ggcggatctc gatttcgcag
     8881 ggcagacctt cacacctgca ggaaatagca tcaccgcttt cacaggaagc tttgacggac
     8941 tggatcatgc catccgcaat gttgtcctga ctgcggacgg cgacggtaca caaggggatt
     9001 cccgcgctat tttcggcgtt gtcggctcag gcggtgcgat ccgaaatctg cattgcacaa
     9061 gcattgagat ttccgggcga gatcgggtgg gcggtctggt tggtgccatg aacaagggca
     9121 ctgttgccgg atgcaccttg acagacagct cgatttccgg acgcagtcat gtgggtggtc
     9181 tggtcggtta tgctgaaaat cactcccgca tcgagaacag tgccacggat agtacggttc
     9241 gcgcatcttc cgatagggca ggcggcattg ccggttcagg cgagggcagt attttccgaa
     9301 gagttcagtc cggtggcatt gtaagttcag caggcagcat ggcaggcggt ttgagcggtt
     9361 ttgcagacgc atccgagttt caggatgcct gcagtaccgc ttcggtttcc gctgtggatc
     9421 gtgcgggcgg acttctgggc gaagcctcct attccaatat agaacgatgc tatgctatcg
     9481 gcatggttga accttccggg aaaaccggtg cattgacggg tggccttatc ggatttgatg
     9541 caggcggttc ggaaataatg gcttcttact ggaatcagga aagcaccggg cagggaggca
     9601 gcgtttctgc cggacgacgc aatactgatc agatggccgg tgagagtgcg caaagcaatt
     9661 acaaaaactg ggactttgac ggtgtctggg caatgccgga agaccttcgg ctcaatcagg
     9721 gctatcctta tctgcgggcg attcccgctg cggttgctgc agacagctgg catcctgccg
     9781 acctcaatcg taactggacg ctggaggaaa atgaggcgct gcattatatg gagcgctggc
     9841 agaaaggagc cgtgccgatg gtctgggctg ttcgcaccgg cttcctttcc cgagcggggc
     9901 actcctatgt ctttgatgct tcgaaaccga tgccggactg ctggagccgc ccttgagggc
     9961 agtgcctttc agacagacag cctccatgct gtaacggtct gattgcagac acacaaaaga
    10021 acagcgcagg cagctgatca gattggctgc ctgcgttttt tgtcgttcag gatacaacta
    10081 aaacacgttt tgaatgtgaa ccgtacggca tgtctcgccg attatataat gggcaagacc
    10141 gttagttttg atagatcgat cgtcaccctt tgcagtataa acgagttgcc ggggtgtcaa
    10201 ccagggcgca gaggctgcct gccgccgcaa gcccaaaaaa tcttttcgca tcaggaatgt
    10261 ttttgttgtg gaaagcgtat aataaggcgt aaaataccta cacgtcagga agtgccggga
    10321 aagcaatgca tccgactgcg aaagcacctc ttttcagttg aattttatct ggcatatgcg
    10381 ctttcagccc ggttttttgc ttaattctct gtaatttttt ggtttatgtt cagtgattga
    10441 tcataaatca gttccgtgtt ataattgccg gtagttataa agtcgtcctc accgctttca
    10501 ttgcatgctt ccgacagtct acaaggttac tggggctgga ggactgcatg aaggttatca
    10561 accgggagac aatggattgc catgggtgat cacaaaatcc gaaaagggct taccctaccc
    10621 ataaaggggg agccgtcgcg tgttgtagca gaaaaaaaag tatcccgcat cgcagtcatg
    10681 gcgaccgact ttgtcggttt aaagccgcgc atgctggttc agccgggcga taccgtgcag
    10741 cggggtcagg cgctttttga ggacaagaaa acaccgggca ctttttttac tgcaccgggc
    10801 gccggtacgg tatctgcggt gaaccgcggt gaaaagcgca tgcttcagtc ggtggtgatc
    10861 gacttgagcg aaagcgaatc gacaggcaac ccttccccgg atgagctgaa aagtttctct
    10921 tcctatacag gtaaaagtgc cgctgatctt tcaggccagg agctgcggga tcttctcaca
    10981 gaagcgggaa tgtggcccgc tttccggacc cgccctttca gcggccggcc gctcattgac
    11041 agcgaacctc attcgatttt tgtgaccgca atggattcaa gccctctggc gcctcccatg
    11101 aatatggtgg ttgaagggcg cgaagcggca ctggcggcgg ggctgacagc gcttaccaag
    11161 ctgaccgggg gcacaatctg gctgtgcgtc gctcccgatg ccggttttat tccttccgat
    11221 ctgcccgctt cggtaaaggt ggagcgtttt tcgggtcccc accccagcgg tacagcaggg
    11281 gttcacattc atacgctcga tccagtgggg cggaataaaa cggtctggca tatcggcctg
    11341 caggatcttc ttgccatggg cgatctgatc cggacgggcg ttctttgcgt ggatcgcctc
    11401 atcagcctgg cgggtcccgg cgtaaaagaa ccgcgtctga tccggacccg tctgggtgcg
    11461 tgcacggact gccttactgc ggaagagctg atcgaaggag aagcccgtgt gatatcaggc
    11521 tccgttttca gtggccggaa atccatgggc gatgtttttg gctacctcgg ccgctttcat
    11581 aatcagatca gcgtgctggc ggaagaccgg gaaaaagtat tcatgggctg gctgacgccg
    11641 ggagcggata aattttcgct gagccggctg tttctatcca aactcttccc gagcaaacgg
    11701 ttcagcctta cgaccagcac caacggaagc ccccgcgcta ttgtgcctgt aggactctat
    11761 gagcaagtga tgccgatgga cattattccc acgctgctgc tccgctctct ttgtgtgctt
    11821 gatgtggatc aggcggaaaa gctgggctgt ctggagcttg acgaagaaga cctggcgctg
    11881 tgcagttttg tggatcccgg aaaagaggat ttcgggtcgc ttctgcgcaa aaaccttgac
    11941 attatagaaa aagaagggta accgcgccca tgaaattcat cagaacttta tttgataagc
    12001 aggcgccgct cttcgaaaaa ggcggcaaac tggaggtgtt ctatcctctt ttcgaagcga
    12061 tagaatcgtt ccacttcacg cccggaaaag tgaccgacgg tgccccccat gtccgtgatg
    12121 ccgttgacct gaaacggatc atgatcacgg tggtggtgtc tttaatgcca gcgatttttc
    12181 tgggattgtg gaatatcggc tatcagatca attccgggct tgcgccgggc gttacgcccg
    12241 aaggctggcg tgcagggctg gtcgcggcac tcgggctggg cttcagccct gcaagttttc
    12301 ttgcctgctt tgtacacggc gccctctatt ttctgcctgt actgattgtc accttcatgg
    12361 taggcggcag tatcgaggtg gcggttgcca ttatccgcaa gcacgatgtg aacgagggct
    12421 tttttgttac aggctttctg attccgctga ttcttccgcc gaccattccc ctgtggcagg
    12481 tggcgcttgc aaccgctttc ggtgtggtca tcggcaagga agttttcggc gggacaggca
    12541 tgaatatttt caatccggcg ctggttaccc gtgcgtttct ctatttcgcc taccctatcc
    12601 agagctcggg cgacaaggtc tgggttgcac tcaatccggc acaggcggct gacggtttca
    12661 gcggcgcgac gctgctggcg cgtgtggcag aaatacctcc cgtcgccgat gggcaggcct
    12721 ggcagactgc agtttccacg atgcagatca atggcaaagc agtgtcctgg ctggatgctt
    12781 ttatcggctg gatccccggt tccatgggcg agacatccac ccttgcctgt ctggtcggcg
    12841 ccgccattct cctgattacc ggtgttgctt cctggcgcat tatggtgtcc atggtcgccg
    12901 gtgcactcgg tcttgtgctt ctgatgaacg ctgccggatc ccagaccaat gagctgatga
    12961 atcttccctt ccactggcat tttgttctgg gcggctttgc tttcggtctg gtatttatgg
    13021 cgaccgaacc tgttaccggg acgtatacgg agacgggcaa atacatttac ggcttcggta
    13081 tcggcgtcat ggcgatactg atccgggcgc tgaacccggc ctatcccgaa ggcgtgatgc
    13141 ttgccattct ctttatgaat attctggctc cattgattga ctatgcggtt gtttcccaaa
    13201 acatcaaaag gagggcggcg cgcagtgcaa tatagcatga aatatatatt cggctttgcc
    13261 gcagcacttt gtttaatctg ctccgtgctg atctccgtcg ccaatgtcgg tcttcgggat
    13321 cggcaggaaa taaacaagca gctggacaaa aagaaaagtg tgctgcaggc ggcgtgtctg
    13381 atccaggccg gcgaaaaaac gaaccgtgcc cgaattgaat cgctttttga aaacatccgc
    13441 gctcgtgtaa tcgatctgtc cgcaggccgg gttctggatg atgtggacag cgcacggttt
    13501 aatgaggaag atattccgga acagccggct cctgccaata aggcgggtat ccgcaccctt
    13561 cgcaatcaga tgctgatcta tgaggtcatg gacggcgaca ggatcagcat gttcgtgctg
    13621 cctattgtgg gcaaggggtt gtggtcgacc atgtacggat ttctggcgct tgatgcggat
    13681 acaaagacca tccgcggact gacctattat gaccagggcg aaacccccgg gctgggcggt
    13741 gaagtgacca atcccaagtg gacggcactg tggcc
//
LOCUS       DAOPWB010000083        13521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4655_length_13521_cov_6.180900, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000083 DAOPWB010000000
VERSION     DAOPWB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13521
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4655_length_13521_cov_6.180900"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PKN92_08640"
     CDS             <1..892
                     /locus_tag="PKN92_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ48899.1"
                     /translation="QDSTEIDSASEWEKKHFRYKPDFQQDSEGTFDTNSLGFRDQERK
                     MPKERGTFRILCIGDDSTVEGSHNNRTYPALMEQKLSATFPGKNIEVINAGIAGITAS
                     DMTQRFFDYASLEPDLVLLQPGASDLLHLYNRRWTNFLPSISVAARLLFSGIFAPSAS
                     SFSDSLKRSQGTALEILCFAFTHTNIPVMLLALPYPDSDRVTRDERQFLKWQSKSALF
                     FPAFSFSDYIGFVKTENLLLQEKAARYHIPFVPMEQGLKDDPAFFGDCFHLTQKGIER
                     KADMACAILEPMIEKGYSAE"
     gene            908..4384
                     /locus_tag="PKN92_08645"
     CDS             908..4384
                     /locus_tag="PKN92_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48900.1"
                     /translation="MEPHPLSTKRPPLAEGVAVLLLVLFSLFVGVAHLRSMRIDGHPS
                     LQYIDNLYGPAVMLAYGHGFVSPDFLLYPKLHEFLKGERESFPRELLPDQIQEAPSSV
                     ASYHQYLLYVVGCFWKITGVSWKNLEILIAFFLVWYTLVLYGFMRLFLGRICSFLLTV
                     FFILSPAVLSIIHDLRDFSKAPFIVTLLLILIWLYQTRLPLRRLLLMSLSVGIIHGIG
                     LGFRQDLIVLVPFFLFALLIIAIKHFKKVQWRVFFPPLIFAFGFLLFAWPMLGRMEGG
                     AQPQHILVQGFAADRMDQLGISQPPYQSLLSGSDHYTFAMLSDYALRKSGMPIYSGLN
                     SKGAEKSGQMWLRESVLLFPADLIARGWAAVLRNQRYADGWTPSFSEQSAIHIFLFNC
                     HHHLSDIFHKTGPVIALCFLLLRFCRAPFDATVLFFSAVYLLGYTALQCHSRHTFHLA
                     FLPLLLSALLLKELAGLIFRPEARQEKYPLPVKQYLIRCIAGVALLSLIIAAPLSAAR
                     LYQTKVLPPLFQSLLDAPRIPVKTNPEKRYGWTFYRISPSAKTVECTNLSSLYKLLSN
                     CYEFTQRAWKVHGRYFVAEFDGNAELDMVVHKYSSYLELNDFSQCIRTPVTKGSGKTR
                     YYFFPAYELASPDHTTFTSWWGRYMGPAFPDELADSFRGLYEIQLPQSISRLISFTSF
                     DGKIPDPLYYSQSIFPDPLYYFQYEQNSHDYINTTWAARRMNRETEKRKAMQALALTA
                     RTEETKRFILYELILTGKLDATLQGLYDLYEPKEDYFSLLKKYLLLVSQSFLSGQYKE
                     DIYWEEGCSLFSKNEMATPFPENNILEKLLFLYTLLLESRPDDTLVIDNVSTLLFHKS
                     SPERAVDFWRTLLQRTRDSAYFYFQLGNAYDAVGNYEKAQILFRGTHRKFPKDNKAAI
                     VYAAGTLDTLDLEKLQQILDNVCESEPSLRLFAAERLERTAVYLSDKGQHNKAADLLI
                     AAIRYAPAPDLVRLKASQQLVGAGKYIEAEQQLVPLLTTDYAEEAVRVLSVATTLSMD
                     FPEQLNYWALKYRKHPDVTAIIDILNGLYAPAARKMFLSQRFNELWAIDLTGLPDSQE
                     SRLFRWYQDSASFLFKMKTEAELSAVFSLLAQEKTLFLSDLQELTSALPEEQHALTEQ
                     AQLLRVFLETIE"
     gene            4418..8863
                     /locus_tag="PKN92_08650"
     CDS             4418..8863
                     /locus_tag="PKN92_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48901.1"
                     /translation="MTKQLSTGFSAKRNELLILLLLFLLSFSIGLFHLRSLRKDNQPS
                     LQYIHSLYGPAVMIAYDYGFTQPDLQLLPELYNFLKGNRESFSPELLPETITERPSSV
                     AAYHRYLLYLVGYFWKITGVSWKRFEILIALFLAWYALVLYAFMRLFFGKKLSASLSL
                     LFILSPAVLLILPDLRDFSKAPFILTILFNLFYLLKTRLSWRKLVFISFMTGAMNGIA
                     IGFRQDPLVFMPLGVFITGLIAFRDHRRPRWKAVSAPAVYLIGFLLLGSPMLGRMEGG
                     AQPQHVLIQGFAENRMDLLGMKEAPYKTLLSGSDLYVFSTVQDYAHRKPGLPHNFAFN
                     SSEAQQSGHQWLLDSLFLFPADIAARGWAAVCRILRYADAFPPSFWEPLAFHRAAYGL
                     HRFLGHFFHAAGLPLALIALLILAIRSPGYALVALGCTAYIMGYTSLQTACRHSFHLS
                     FFPLLIAAFLVEQTRGYLTKVWSSKTFVCTIQRKKLLAAIFVLFASFFITLSPLFPLR
                     IYQAQKIAPILNACLQAPLTPIFVSEDERCGWTVFSPTRKHNLSSKTDLQGLYTLLAA
                     CFNPELRLWNVRSRYLMAEFDGEMDSPYLLHIYESLIDSCNFSQLMRVPHSRKKGTVI
                     KYFFPAYELFMIDVFEQTPLARNKFTGLALPAAAASCFRGLYEVSVPEEVTHLIQFVA
                     VDDELPRPLYRRLSWYPDLLYYYQEEQSKPNSTLLAEAALRFNQLDKARLFFSSALLV
                     ATEPAIQFSALDKLIALGDLEDALEGALRMHVSLEKDKRILIEKLEWIASLGLYQDKI
                     DVSVPAFDALIQLDPEQDAEFRAMKADAFERIAAWEQASAVWLDYLKYHPQDLDCIQK
                     TEILLSSHCSQEQSEAFWETLTREHPGHALFFLHLGKAYDRAEKEEAAIRCFSHAHQL
                     DPANLEISIYAAAANADESATGQSAIDMRHMTLSDAHYKKLAIGRLERSAFYRSQKKE
                     FGKAASLLELAATLADDPESCMLKASHLWIGAGDYERAKDGFTRLLSGSLMQEALSGL
                     VLIAQHQNDLKEKFLFWSQLSDQFPDNSFFKDHCESIRISAARAFLLSGDFIAASDLY
                     NSSGADHLSVADSFLKAVTCFIRIDQDESAALHSFSEKLNPAESLSVFADILRLITVL
                     NNAGLSEKAFILADFFISLLSGADQDYLWLRDLKTLDPAAVQETSTLFQERGGMLLES
                     GKSDEGLSFLQTAASLQQDNSFLWLTLSRACFTLGQKEKAIYFCEKALMADPLSPAAS
                     EAAKMLYWSLKIRDDRHWAQWSAENPNNAPAFYYHILNQGLASSAGDALHLLNTNTTK
                     SAAHRDSELVSGILELIEGNPDAGFALITKNTVNSAFLPDVVAFLDETATSFAAAEKF
                     DRAEVLLHAATQLEKENKYYFFRLAELLVQQEKYEEALPYLTGILEEAPDSSKSAVLL
                     DQVFAAQNNQEKQREFWKGLCTKYPDAQVPLEHLRKAEAAPQPLK"
     gene            8904..10091
                     /locus_tag="PKN92_08655"
     CDS             8904..10091
                     /locus_tag="PKN92_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNZ48902.1"
                     /translation="MQAKKISERITWMGAVDWNRRLFDSLIPLPEGTSYNAYLLRGSE
                     RCALIDTVDPAKSDLLMAQLANVEKIDFIVSQHSEQDHAGTIPLVMEKYPQAKVLCSD
                     KARDMLIDHLHLDPERIQAVADGDIISLGDRSLRFVYTPWVHWPETMCTFCEEEGILF
                     SCDLFGSHIATSALYAEENPQVELAAKRYYAEIMMPFRSFIKKHLDKIQTLNFSMIAP
                     SHGPVYNKPDFILNAYKNWISNDVANKVVIPYISMHGSTEAMVTHLVSALDARGIEVH
                     PFDLSVTDIGELAMALVDAATIVIGTPTVLFGPHPLVFSATHLANALRPKLRFAAIIG
                     SYGWGTKAAEQLTELIPHLKVDVLGVVMCKGFPREEDIKNLDTLAQNILEQHRGLEKA
                     VAE"
     gene            10106..10759
                     /locus_tag="PKN92_08660"
     CDS             10106..10759
                     /locus_tag="PKN92_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNZ48903.1"
                     /translation="MIDAYFFDLDGTLVDTEILWVEATEMLAKDMGHPLSHETALRLV
                     YGISWPGVYEGLLKEIPGIDWTLDQMGDILAPYFLQLRDSRDVRILSSIKLLKTLAKE
                     HPVAIVSGSYCTDVKAAIDLMDIMPYLRFYLGHEDYHPGKPDPACYLAAAAKMGVSPK
                     NCVVFEDSTAGLTAGIDAGMYAVALSRPGRPAQDYSRAHLILEDLADYHPSRLICAH"
     gene            10872..>13521
                     /locus_tag="PKN92_08665"
     CDS             10872..>13521
                     /locus_tag="PKN92_08665"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HNZ48904.1"
                     /translation="MRPILFALFFSSCFLLYSFPSGGTAVNEEVHIQGKYLDWSFLPE
                     DGGILSGLNRIGSVYNLSDTEGLLQEGFGVGSYYHPNRRLNERLEILDSKASPLISSS
                     YECDGPNIKGLAVTKLIEPIINESSIRVTWTIKNNGSESQWISPWVRNAIHYQEDSRV
                     DLPTVEGMESPRSELFLPAARNWASLTDTAKQETVYGIFHADHVHAFGAVSNDEGHLN
                     TIHTAYVPRFLKPGDAWTTIYRLNVARGLSHVDFATDEFALQLDYSQNQLTALLAPAK
                     SMDALYLDARIVAPNKRVWKLPRKKFNASPENLSRFTYEWEAPADGTYEFLAQISTES
                     GILPLGEDTGSPHGGIDAVFSVGNAGKEKLAPWTDAPFLLDRQGRIITRDLAVEGDVR
                     IFFASSLEKIFQEDSVRSSGKVNPVHRICLAQNESESFQLCVAPAEDPLEGVTVHISD
                     LIHSDGKMRINASDIDICNVRYHRIQIPSHYEGPTGWFPDALPPHRAFEAHPGEVTPV
                     WITLKAHKDTKPGLYKGMIEIKAGNGGPWKVQIEAEVFDFALPDTPTLKTDFGFSLES
                     LASLPGGGKPQRQLLQAWLDNALNHKITLRELCQFPRESGDYTAALSTYTPHLENCIR
                     KSASTFFVPATLLDAPAQLEAVNAYVRSHNLTGKAFTQLAYEPETPAWGRVLERMQQW
                     KNLAPDIAVTVSTTGLKAFIPEALDIWTLHAQVMDTTQNADILARTSEGGEVWWYVNH
                     MPPRPYGNFFIDFSPMEHRILFWQSWALGIKGMYYWSVNHWQNPQDPFNYPSDVTPVN
                     GNGMLLYPGDQGPINSIRWEVIRDGIEDYDYLALLTEYRRRLSSLRNADALMKQAAEA
                     YNLSEIIPSLVSYTQDPVRLQTKREAL"
BASE COUNT         3278 a         3422 c         3094 g         3727 t
ORIGIN      
        1 acaggacagc acggaaattg attctgcttc agaatgggaa aagaaacact tccgttacaa
       61 accggatttt cagcaggaca gcgaaggaac ttttgacacg aacagcctcg gttttcgcga
      121 tcaggaaaga aaaatgccca aagaaagggg cactttccgt attctgtgca tcggtgatga
      181 ttcgactgtg gaaggatcgc ataacaaccg gacatatccg gctcttatgg aacaaaaact
      241 ctctgccacc ttccccggca aaaacattga agtgatcaac gcaggcattg caggcatcac
      301 cgcttccgat atgacacagc gcttctttga ttatgcatcg ctggaaccgg atcttgtatt
      361 gcttcaaccg ggagcgagtg atcttctgca cctttacaat agaagatgga caaactttct
      421 gccctccatc tccgtcgccg cccggctgct tttttccggc atttttgcac cgtccgcttc
      481 ctctttcagt gactcactca agcggtctca agggaccgct cttgaaatcc tgtgctttgc
      541 tttcacgcat acaaatatac ccgtcatgct gctcgctctg ccatatcccg attcggacag
      601 agtgactcgg gacgaacggc agttccttaa atggcaaagc aaatccgctc tcttctttcc
      661 cgcattctca ttctccgact acataggctt tgtgaaaaca gaaaatcttc ttttacagga
      721 aaaagccgcg cgatatcata tcccctttgt tcccatggaa caaggactga aggacgatcc
      781 tgcttttttt ggcgactgtt ttcacctgac tcaaaaaggt attgagagaa aagcggacat
      841 ggcgtgtgct attcttgaac cgatgatcga gaagggatat tctgctgaat aaaaaggcta
      901 tgcttccatg gaaccgcacc cgctaagcac taaaagacct ccattggctg aaggcgttgc
      961 agtgctgctt cttgtgcttt tcagtctctt tgtcggcgtg gctcacctcc gatccatgcg
     1021 catagacggg catccttctt tacagtatat tgacaatctg tacggtcctg ccgtcatgct
     1081 tgcctatggg cacggctttg tttcgcctga ttttctccta tacccgaagc ttcatgaatt
     1141 tctgaaagga gaacgcgagt cattcccccg tgaattgctt ccggatcaga ttcaggaggc
     1201 gccttcaagc gtcgcatcct accaccagta tttactttac gttgtcggtt gtttctggaa
     1261 aataaccggt gtctcctgga aaaatctgga aatccttatt gccttctttc ttgtctggta
     1321 tacccttgtt ttgtacggct ttatgcgcct ttttctgggg cgtatctgtt cttttttatt
     1381 gactgttttt tttattcttt ccccggctgt cctctctatc attcatgacc tgcgtgattt
     1441 cagcaaagct ccttttatag tgacgctgct ccttatttta atctggctct atcaaaccag
     1501 actgcccctt cgccggcttc tcctgatgtc tctgtctgtg ggaataattc atggtattgg
     1561 gctgggtttc aggcaggatc tgattgttct tgttcccttt tttctttttg cccttctgat
     1621 tattgccatt aaacacttca aaaaagtgca atggcgtgtt ttttttcctc cgcttatttt
     1681 tgctttcggc tttctgctct ttgcctggcc tatgctcggg cggatggaag gcggggcgca
     1741 gccgcagcat atactggtac agggatttgc tgctgataga atggatcagc tgggaatttc
     1801 acaaccgcct taccagtccc tgttatccgg ttccgaccat tacacttttg caatgctctc
     1861 cgactatgcg cttcgcaaat caggcatgcc catttattcc ggtttaaact ccaagggtgc
     1921 ggaaaaaagc ggccaaatgt ggctcagaga aagcgttctg cttttccccg ctgatttgat
     1981 tgcccgcggt tgggcggctg tgcttagaaa ccagcgctat gccgacggat ggacaccttc
     2041 gttttcagaa cagtcggcaa tacacatatt tctgttcaat tgccaccatc atttatccga
     2101 tatatttcac aaaacagggc ctgttattgc cttgtgcttt cttctgctcc gtttttgcag
     2161 agctcctttt gatgcaacag ttcttttttt cagtgccgtg tatctgctgg ggtatacagc
     2221 actgcaatgc cattcccggc atacttttca tctggccttc ctgccgcttc tgctgtctgc
     2281 gctcctgctg aaagaattgg ctggtttaat tttcagaccc gaagccagac aagaaaaata
     2341 tcctctgcct gttaaacagt atcttataag gtgcattgcc ggcgttgctc tgctgtcctt
     2401 gataattgcc gccccgctgt ccgctgcacg cctgtatcaa actaaagtac tgcctcctct
     2461 ttttcagagt ctgctggatg ccccaagaat tcctgttaaa acaaacccgg aaaagcggta
     2521 cggctggact ttttaccgaa tatcccccag tgctaaaact gtcgaatgta caaatctttc
     2581 atctttatac aaattattat cgaattgtta tgaattcact caaagggcct ggaaggtgca
     2641 tgggcgttac ttcgttgcag aattcgatgg aaacgcagaa ctggacatgg ttgtgcacaa
     2701 atacagttcc tatcttgaat taaatgattt ttcacaatgt atccggacgc ccgtcaccaa
     2761 gggatccggg aaaacacgct attatttttt ccccgcttac gagcttgctt caccggacca
     2821 cacaacattt acgtcctggt ggggcaggta tatgggtccg gcatttcctg acgaactggc
     2881 tgactctttc cgcggactct atgaaataca acttccgcaa tctatcagca gattaataag
     2941 ctttacctct tttgacggca aaatccctga cccgttatat tactctcaat caatttttcc
     3001 cgacccgctg tattattttc aatacgaaca gaacagccat gactatatta atactacctg
     3061 ggcagcacgc cgcatgaatc gggaaacaga aaagagaaag gcaatgcagg cactggcatt
     3121 gactgcgcgt acggaagaaa caaaacgctt tattctgtac gaattaattc tgaccggaaa
     3181 acttgacgca acccttcagg gtctttacga tttgtatgaa ccgaaagaag attatttttc
     3241 tcttctaaaa aaatatcttc tgctggtaag ccagtcgttt ttatccgggc aatataaaga
     3301 agatatctac tgggaagagg gctgcagcct gttttctaaa aatgagatgg caacgccctt
     3361 ccctgaaaac aatatacttg aaaaacttct tttcctttac actcttcttc tggaaagccg
     3421 ccccgacgac actttggtta tagacaatgt gagcacactg ctttttcaca aatcttctcc
     3481 ggagcgcgct gtggacttct ggcggacatt gctgcaaagg actcgggatt cggcgtattt
     3541 ttatttccag ttaggaaacg cctacgatgc tgtgggcaac tatgaaaaag cacagatact
     3601 gtttcgtggg acacacagaa aatttccgaa ggataacaaa gccgcaatcg tttatgctgc
     3661 cggcactctg gatacattgg atctggaaaa gctacaacaa atactggaca atgtctgtga
     3721 aagcgaaccc tcgctgcgcc ttttcgctgc tgagcggctg gagcgaaccg cagtttattt
     3781 gtcagataaa gggcagcata acaaagcagc ggatttgttg atagccgcca ttcgctatgc
     3841 acctgctcca gatctggtgc gacttaaagc atcgcagcaa ctggtcggcg ccggaaaata
     3901 tattgaagca gaacaacaac tcgtgcctct gctcactaca gactatgctg aagaagcggt
     3961 ccgagtccta tccgttgcaa ctactttatc aatggatttc cctgagcagt tgaactactg
     4021 ggcgcttaaa tatcgaaaac accctgatgt aactgcaata atagatatcc tgaacgggct
     4081 gtatgcacct gcagcgagaa agatgttcct ttctcagcgg tttaatgaac tatgggcgat
     4141 tgatctgacc ggtctccctg attcacaaga gagcaggctc ttcaggtggt atcaggacag
     4201 cgcatctttt ctgttcaaga tgaaaacgga ggctgaattg tcagctgttt tttcattact
     4261 tgctcaagaa aaaactctat tcctgtctga cctgcaggaa ttgacatccg ctctgcccga
     4321 agaacagcat gcgctgacag agcaggcgca attgctgcgt gtatttttag aaacgattga
     4381 gtaagcaggg tctatccctc aatcaaaaag atttctggtg actaaacaac tgagtaccgg
     4441 tttttcggca aaacgcaacg aactcctgat tctgctgctg ctgtttttat tgagcttcag
     4501 catagggctt tttcatcttc gttctttgcg caaagacaat cagccgtcac tgcagtatat
     4561 acacagtctt tatggtccgg ccgtcatgat tgcctatgat tacggcttta cccaacccga
     4621 tcttcaattg ttgccggagc tttacaattt cctgaaggga aatcgggaat ctttttctcc
     4681 ggaattactt ccggaaacaa tcaccgagcg cccatcctct gttgctgcat accaccgtta
     4741 tttattatat ttggttggtt atttctggaa gataaccggc gtttcctgga aacgatttga
     4801 aattctcatc gccctgtttc ttgcctggta cgcccttgtc ctctacgctt ttatgcgtct
     4861 tttttttggg aaaaaacttt ccgcttccct ctctcttcta ttcatcctgt ctcctgccgt
     4921 tctgctaatc cttcctgatt tacgcgactt cagcaaagca ccttttattc tcacaattct
     4981 ctttaattta ttttatctcc taaaaaccag gctatcctgg cgcaaacttg tctttatatc
     5041 ttttatgacg ggtgccatga atggaatagc catcggtttt cgtcaggatc cgctggtttt
     5101 catgcctttg ggtgttttta taaccggtct catcgctttt cgggatcacc gccgcccacg
     5161 ctggaaggcg gtatcagccc ccgctgttta tctgattggt ttcttactgt tgggatcacc
     5221 catgctcggg cgtatggaag gcggtgcgca gccgcagcat gttctgattc aaggctttgc
     5281 agaaaatcgt atggaccttc tgggaatgaa agaagctccg tataaaactc tgctgtccgg
     5341 ttcggattta tatgtcttct caacagttca ggattatgca caccgcaagc ccggtcttcc
     5401 ccataatttt gcctttaatt cttccgaggc acaacaaagc gggcatcagt ggctccttga
     5461 cagcctgttt ctttttcccg ctgatatagc agcccgtgga tgggctgctg tctgccgaat
     5521 tttacgttat gctgatgctt ttcctccttc cttctgggag cccctcgcat ttcatcgggc
     5581 ggcgtatggg ttgcaccgct ttttagggca cttcttccat gcagccggtc ttccgctggc
     5641 actaatcgct ttactgatcc ttgccatccg ctccccggga tatgcactag tagccttggg
     5701 ctgtactgcc tatattatgg gctacacttc attgcaaaca gcgtgcaggc acagcttcca
     5761 tctttcgttt ttccctttgt taatcgccgc tttcctcgtg gaacagacca gaggctatct
     5821 gacaaaggtc tggtcctcta aaacatttgt ctgtacgatc caacgcaaaa agctccttgc
     5881 tgctattttt gttctttttg ccagtttctt cattacacta agccctctat ttccactccg
     5941 aatctatcag gcacaaaaaa ttgcgcctat ccttaacgcc tgcctgcaag ctccactaac
     6001 gcctattttt gtttctgaag atgaacgctg cggctggact gttttttcac cgacacgaaa
     6061 acacaactta tcctctaaaa ccgatctcca ggggctgtat acgcttcttg cagcgtgttt
     6121 taatcccgag ctgagactgt ggaacgttcg atctcgttat ctcatggcag aatttgatgg
     6181 cgaaatggat tctccctacc ttttgcatat ctatgagagt cttatagaca gttgtaattt
     6241 ttctcagctt atgagagttc cgcattcccg taaaaaggga acggtcataa aatacttttt
     6301 cccggcatat gagttgttca tgattgatgt attcgaacag acccctttgg cacgcaataa
     6361 attcacaggt cttgctttac cagctgcagc tgcctcctgt tttcgcggat tatacgaagt
     6421 aagcgtgcct gaagaggtta cacatttgat acagttcgtt gctgtcgatg atgaattgcc
     6481 gcgccctctg taccgacgtc tgagctggta tcctgatctt ctttattatt atcaggaaga
     6541 acaaagcaaa ccgaacagta cgctgctggc tgaagctgcg ctgcgtttta atcagttaga
     6601 taaagcccgc ctctttttct cgtcagcact gctggtagcc acagaaccgg ctattcagtt
     6661 ttcagcattg gataagctga ttgctctggg agaccttgaa gacgcattag aaggtgcgct
     6721 gcgcatgcac gtgtccctag aaaaagacaa gcgcatcctg attgaaaagc tagagtggat
     6781 tgccagcctc ggtctttatc aggataagat cgatgtcagc gtaccggctt tcgatgcact
     6841 catacagctg gatccggaac aggatgctga gttccgcgca atgaaagcgg atgcctttga
     6901 acggattgct gcgtgggaac aggcgtctgc cgtctggctc gactatctta agtaccaccc
     6961 tcaagatttg gattgtatac aaaagacaga aatcctgctg tcctcacatt gctcccagga
     7021 gcagtccgag gcgttctggg aaacacttac ccgcgaacat cccggccatg cacttttttt
     7081 ccttcatctt ggcaaagcct atgatcgagc agaaaaagag gaggcggcga tacgctgttt
     7141 ctctcacgct catcagctgg atcctgccaa tctggaaatc agcatctatg ccgccgccgc
     7201 caatgccgat gaatctgcta cgggccagtc tgccattgat atgcggcaca tgacactttc
     7261 agacgcccat tataaaaagc tggcaatagg ccgcctggag cgttccgctt tctaccgttc
     7321 acaaaaaaaa gaattcggaa aagccgcgag cttgctcgaa ctggcggcta ctctcgcaga
     7381 cgatcccgaa tcctgcatgc ttaaggcaag tcatctgtgg attggtgccg gtgattatga
     7441 gcgcgctaaa gatggattta cccgtcttct ttcaggttcg ctcatgcagg aagcattgtc
     7501 agggcttgta ctgatcgcac agcatcagaa tgatttaaaa gaaaagttcc ttttctggtc
     7561 acagctttca gatcaatttc ctgacaattc attttttaaa gatcattgcg aaagcatccg
     7621 aatttcagca gcccgggcat ttctgctctc aggcgatttt atagccgcct cggatttata
     7681 taacagcagc ggtgcggatc atctttctgt ggctgattcg tttttaaaag cagtgacgtg
     7741 ttttatccgg atcgaccaag acgagagtgc ggcactgcac agtttcagtg aaaaattaaa
     7801 tccggcagaa agcctcagcg tatttgctga tatcctccgc ctcattacag tgctgaataa
     7861 tgccggtctc agtgaaaaag cctttatcct tgccgacttt tttatttctt tgctgagcgg
     7921 tgcggatcag gattatctct ggctccgtga tcttaaaaca ctcgatcctg cagccgttca
     7981 ggaaacctcc acgctgttcc aggaacgggg cggcatgttg ctggaaagcg gaaaatcaga
     8041 cgaaggactt tcttttttgc aaactgccgc ttctttgcaa caggacaact ctttcctttg
     8101 gcttacgctc agtcgggcgt gtttcacgct cgggcagaaa gaaaaagcga tctatttctg
     8161 cgaaaaagcg ttgatggcag acccgctatc ccctgccgcc tcagaagcag cgaaaatgtt
     8221 gtactggtct ttaaaaatac gggacgaccg gcattgggcg caatggagcg ctgaaaaccc
     8281 aaacaacgcc cccgcctttt attaccatat tctcaatcag gggcttgcct cgagcgccgg
     8341 cgatgcgctg catttactga acaccaatac cacgaaaagc gccgcacata gagattctga
     8401 actcgtttcc ggtatactgg agctgataga aggcaacccg gacgccgggt ttgctctgat
     8461 aacgaagaat accgttaaca gcgccttcct tcctgatgtg gtggcgtttc tggatgaaac
     8521 tgccacatcc tttgctgcag cggaaaaatt tgaccgggcg gaagtgctgc tgcatgcggc
     8581 aactcagctt gaaaaggaaa acaagtacta tttttttcgt cttgcagaac ttctggttca
     8641 gcaggagaaa tacgaggaag cccttcccta tttgacgggc attcttgaag aagccccgga
     8701 ttcttccaag agcgctgttt tgctggatca ggtttttgcg gcacaaaata atcaggaaaa
     8761 gcaacgcgag ttctggaaag ggctgtgcac gaaatacccg gatgcgcagg tccctttgga
     8821 gcacctccgt aaagcggagg ctgctccaca acccctaaaa tagtaaagtc aggaagtgca
     8881 actttcatct caaggaggca gcaatgcagg caaaaaaaat atctgaacgc atcacctgga
     8941 tgggggctgt agattggaat cggcgtcttt tcgattctct tatccccctt cctgaaggta
     9001 ccagctacaa tgcttatctg ctcagaggtt ctgagcggtg tgctctgatc gatactgttg
     9061 acccagcgaa atcggatctg ctgatggcgc agctggcaaa cgtggaaaaa atcgacttta
     9121 tagtctccca gcattctgaa caggatcacg ccggaacaat tccccttgtc atggagaaat
     9181 atccgcaggc aaaagtgctg tgttcagata aggcgcggga catgctgatc gatcatcttc
     9241 atctggatcc cgaacgcatt caagcagttg ccgacggaga catcatctcg ctgggtgatc
     9301 gaagcttgcg ttttgtctat acgccctggg tgcattggcc ggaaacgatg tgcacgttct
     9361 gcgaggaaga aggcatcctt ttttcctgcg accttttcgg atcacacatt gccacctccg
     9421 ccctttatgc ggaagaaaac ccgcaggtcg aactggcggc gaagcggtat tatgctgaaa
     9481 tcatgatgcc gttccggagc tttatcaaga agcatctgga caagatacaa acgctgaatt
     9541 tttctatgat tgctcccagc cacggacctg tttacaataa accggacttc atcctgaatg
     9601 cctacaaaaa ctggatttca aacgacgttg ccaataaagt agtcatcccc tatatttcca
     9661 tgcatggcag cactgaagcg atggtgaccc atctggtgag tgcgcttgat gcccggggta
     9721 ttgaagtcca tccctttgat ctttccgtta cggacatagg cgaactggcg atggcgctcg
     9781 tggatgcagc cacaattgtg attgggacac ccactgttct cttcggaccg catccgcttg
     9841 tcttttccgc gacccacctc gcaaatgcgc tcaggccaaa attgcgtttt gccgctatta
     9901 tcggctccta cggctggggc actaaagcag ccgagcaact gacagagctg attccgcatc
     9961 ttaaggtgga cgtacttgga gttgtcatgt gtaaaggttt tccgcgggaa gaggacatca
    10021 agaatctgga taccctcgct cagaatattc ttgaacaaca caggggactg gaaaaagcag
    10081 tcgctgaata gaaagatttt tttgaatgat agacgcctac ttttttgact tggatggcac
    10141 tctggtcgac acggagattt tgtgggtgga agccacagaa atgcttgcca aagacatggg
    10201 acatccgctg agtcatgaaa ccgctttgag gctggtttac ggcatttcct ggcccggcgt
    10261 ttatgaagga ctcttaaaag aaattcccgg tattgactgg acccttgatc agatgggtga
    10321 cattcttgcc ccttatttcc ttcaattacg ggacagcaga gacgtgcgca ttctcagttc
    10381 cattaagctt cttaaaacgc tggcgaagga gcatcctgtc gccattgttt ccggttctta
    10441 ctgcacagat gtaaaggcgg cgattgatct gatggatatc atgccctacc tgcgatttta
    10501 tctgggtcat gaagattacc atcccggaaa gcctgatccc gcctgctacc ttgccgccgc
    10561 agctaaaatg ggggtatccc cgaaaaactg cgtcgtattt gaagactcaa ccgcaggcct
    10621 tactgccggc attgacgcag gaatgtatgc ggttgcactg tcccgcccgg gaaggcctgc
    10681 gcaggattat tccagagcac atctcattct ggaagatctg gcggattatc atccttcccg
    10741 cctgatttgt gctcactgac cgcccgtaat ttcgctcaac gccgatcgac tgcctcgatc
    10801 ggcttttatt ttaaatccgg tataatactc caacatcctg tttatccttt tctgcaagtg
    10861 aaagaagagt catgcgcccc attctgtttg cactattttt cagttcctgt tttcttcttt
    10921 attctttccc ctcaggcgga accgctgtca atgaagaagt gcatatacag ggaaaatatc
    10981 tggactggtc tttcctccct gaagacggag gaattctcag cggattaaac agaatcggga
    11041 gtgtctataa tctctccgat acggaagggc ttttacagga aggcttcggt gtcgggagtt
    11101 attatcatcc caatcgtcgc ttgaatgaac gtctggaaat tctggacagc aaagcatcgc
    11161 cgcttatttc ctcctcctat gaatgtgacg gtcctaatat taaagggctc gccgtaacaa
    11221 aactcataga accaatcatt aatgaatcct ctatccgagt tacctggacc atcaaaaata
    11281 atggcagtga aagccaatgg atttctccgt gggtgcgaaa tgccattcac tatcaggaag
    11341 actcccgtgt agatttgccg acagtggaag gcatggaatc gcctcgttca gaactgtttt
    11401 tgccggctgc gaggaactgg gcttccctga cagacacggc aaagcaggaa acagtctacg
    11461 gaattttcca tgctgatcat gttcatgctt tcggcgctgt cagcaatgat gaaggtcatc
    11521 tgaatactat tcacacggcc tatgtacccc gctttttaaa accgggagat gcctggacga
    11581 ctatctaccg gcttaatgtt gcccggggcc tttcccatgt tgactttgca acagatgaat
    11641 ttgcgctcca gcttgattac agccagaatc aattgaccgc ccttctggca ccagctaagt
    11701 ctatggatgc tctgtacctg gatgcccgaa ttgtggcacc caacaagcga gtctggaaac
    11761 ttccgcgaaa aaaattcaac gcctcccctg aaaatctgag ccgcttcact tatgagtggg
    11821 aagcgcctgc cgacggaaca tatgagtttc tggcacagat cagcacagaa agcggcatcc
    11881 tgcctctggg tgaagacacc ggctctcctc atggtggcat tgatgctgtt ttcagtgtcg
    11941 gtaatgcggg aaaagaaaaa ctcgccccct ggacagatgc gccttttctt ctggacagac
    12001 aagggcgcat tatcacccgg gatcttgccg tggaagggga cgtccggatt ttcttcgcat
    12061 catctctgga aaagatattt caggaagaca gcgtccgttc ttcgggcaaa gtaaatccag
    12121 ttcacaggat ctgtctggcg caaaatgaat cagaatcctt ccaactttgt gtcgctccgg
    12181 cagaagatcc tctggagggg gtcaccgttc acatatctga tttaattcat tccgacggga
    12241 aaatgcgtat taacgcatcg gatattgaca tatgcaacgt ccgttatcac cgaattcaga
    12301 ttcccagcca ttatgaaggc cccaccggct ggtttcctga tgcattgccg ccgcaccggg
    12361 cttttgaggc gcatccgggc gaagtcactc ctgtctggat tacgctaaaa gcccataaag
    12421 acacaaagcc cggtttatac aaaggcatga tagaaatcaa agcaggcaac ggagggccct
    12481 ggaaagttca gattgaagcg gaagtttttg attttgcact gcccgacaca ccgactttaa
    12541 aaacagactt cggcttttct cttgaatcct tggcatcgct gcccggaggg ggcaagccgc
    12601 aacgtcagct cctgcaggcg tggctcgata atgcgctgaa ccataagatc actttgcggg
    12661 aattatgcca gttcccgcgt gaatccggtg attacaccgc agcactgtca acgtatacac
    12721 cgcatctgga aaattgcatc aggaagagtg cgtcaacctt ttttgttccg gcaactctgc
    12781 ttgatgcacc ggcacagctc gaagcggtaa acgcctatgt ccgcagccat aatcttacgg
    12841 gaaaagcgtt cactcagctc gcttatgaac ctgaaactcc tgcctgggga cgtgtattgg
    12901 aaagaatgca gcaatggaag aatctggcac cggacattgc ggtaactgtc agcacaaccg
    12961 gtctgaaagc cttcattcct gaagcgctgg acatctggac acttcatgcg caggtaatgg
    13021 ataccacaca gaacgctgat attcttgcaa gaacttcaga aggcggagag gtctggtggt
    13081 atgtcaatca tatgcctccc cgtccttatg gtaatttctt tattgatttt tcgcccatgg
    13141 aacatcgtat cctattttgg caatcctggg cgcttggcat aaagggaatg tattactgga
    13201 gtgtcaacca ctggcaaaac ccgcaggacc ctttcaatta tccttctgat gttacgccgg
    13261 tcaatggcaa cgggatgctg ttgtaccccg gtgaccaggg tcccataaac tcgatccgct
    13321 gggaagtcat tcgcgacggt attgaagatt atgactatct cgctctgctc accgaataca
    13381 ggcgtcgcct ctcttcactc cgcaatgcgg acgctcttat gaaacaggcg gccgaagcct
    13441 ataatctttc cgagatcatt ccaagcctgg tttcttatac gcaggatcct gtacggcttc
    13501 agacaaaacg agaagcgctt g
//
LOCUS       DAOPWB010000084        13139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4830_length_13139_cov_5.562901, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000084 DAOPWB010000000
VERSION     DAOPWB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4830_length_13139_cov_5.562901"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKN92_08670"
     CDS             complement(<1..290)
                     /locus_tag="PKN92_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48905.1"
                     /translation="MQDAAEPAVSIHFLAPLVYVCIFSFQHRYDGVAGFAFADLINGD
                     DIVFLFFASQMIVDVSLPYPCTSQHRFEDFRDGGFVFGNSGIKGRLKIRPYPG"
     gene            459..1472
                     /locus_tag="PKN92_08675"
     CDS             459..1472
                     /locus_tag="PKN92_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48906.1"
                     /translation="MLIGYPGQIIEIEVSGGDDRDYVFNTAVDPLDGCGGGCPPTQDC
                     MDRFGNSSRVCVTNISNRNIPYLWDASGCVESIEPDPDTDTGLCDKCYVKLKKEYIET
                     AAEFTVSVTRHGASQEELQCENFAYAEWKGVVLPPPQFTMSISTSPQTAILLLSGPDA
                     EGSAWLREDKSQNPTVPQDTLKQRSSDPSDRSVGVDYRVHLQYTDPLAPVDYDSTNSL
                     HFAAMRTLDLLGRDILISNHLYFTGIPAPPVGPVFSGVHGLAYEGYDFIILSSVEVVD
                     VKQTFAHEIGHCCGLADNQPLVSHNLMLSGLGPSGFTYGMDVHSQFSQNLKLDDGSED
                     WHD"
     gene            1550..2524
                     /locus_tag="PKN92_08680"
     CDS             1550..2524
                     /locus_tag="PKN92_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48907.1"
                     /translation="MNKNAKYSQKIAGGATLPHRWSIRKVLYDMAVISSMTVIIFCVL
                     IVLLLSPMVLCQVEPGGVFLYPSSSPDVPRPHDPDLSSFDDPFLKEIYERTMIGIRTA
                     EIHPDGPAARVQAIADFCAVPKPPEEIDYIVAAAREAVELQQWSRYRAALWCTQAGVE
                     IEVRLVALSREMLQAPRGEKLSDEHGLAIYDTLEFLGRTPIESAADLLMEATTLEFWG
                     PGPLRSRRLRPDTQQSVTMLRSQAVRNLGKLPSSLSLPRLEELAKQYPDKSATSAPVE
                     EYRFEMGAGYTIAEQIYNVKVREGIPGAMHPFVELYREPSEPMVEPVL"
     gene            2595..3440
                     /locus_tag="PKN92_08685"
     CDS             2595..3440
                     /locus_tag="PKN92_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48908.1"
                     /translation="MYNDYFSAFHLGPCRWGVSLVLLVVLLAFSAAGTAEQLLLCNFN
                     PAPELPYESTSFEQDALYAEAKQLDKKDQALAACRALRQDSSAPWELQRWATLREIEI
                     LTVMDQEHAALDLGKAWLRQNPDDPGALEIRVELGKIVAQRRHSGFMPTLQEVDEIFT
                     DIFNNHDPDSWHVVTAHIQYAQKLQKFYFSDRDWTVYARCIEQLYIARQCLEKRAKDE
                     TLSAQERRRAESYLQDHVLPWLSRALGFGIKGRPRELSPEEKELIRHLEEEGTHIRRS
                     VPEEE"
     gene            3767..4201
                     /locus_tag="PKN92_08690"
     CDS             3767..4201
                     /locus_tag="PKN92_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48909.1"
                     /translation="MNKNLLIGFAVLLVVFLVLPIAASFLRGGSSGSAAPVISAEEAG
                     PPKWNSENLVGTAWSVKTPDIPVAVTINLAAGGQAIATAPAILAPMVKAHLGTDTIVG
                     TWRVEGAKVIASVNLKGKEVSVACDIRGDRLFYQDKEIKRVG"
     gene            4229..5032
                     /gene="larE"
                     /locus_tag="PKN92_08695"
     CDS             4229..5032
                     /gene="larE"
                     /locus_tag="PKN92_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNZ48910.1"
                     /translation="MTATALAPLEKEAKLLEQLRRYPSAALAYSGGVDSTYLADVAHE
                     ALGENLLLLIAEAATMPREEYDFAVAQAKNRGWRLESFSPAVFDQVSFVRNSDMRCYY
                     CKSEVFAVMAQHAAAHGITVLLHGETAEDAFDTTRVGIRAAREAGVHAPLADQGFTKA
                     DIRERSRIRQLPTWDKASFACLATRVPTGTPLSTELLNRIETAETVLKDSGFHQYRAR
                     HHGNLCRIEIEPEEFPRLLDPALRQQIITAIQYAGYTYVTLDLKGYGRH"
     gene            5329..6243
                     /gene="ispH"
                     /locus_tag="PKN92_08700"
     CDS             5329..6243
                     /gene="ispH"
                     /locus_tag="PKN92_08700"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNZ48911.1"
                     /translation="MHILLAKPRGFCAGVDRAIKIVEHALERYGAPVYVLKHIVHNAH
                     VVEKLRRQGAVFVKTLEEVPEESRLLFSAHGVSPDRHAEAKARHLHVIDATCPLVAKV
                     HKEIRRFAEDGYTIIYIGDAGHDETLGAVGWAPEHIRLVRTEEDAWKVSVADPERVAY
                     ITQTTLSIVDCEDVANVLRARFPKITAPKKPDICYATSNRQMAVLSLVPEVDKVLVVG
                     DPESANSCRLRDIALKLGKPSYLIQDASRIDKAWLCGDDTILLTSGASAPDHLIREVI
                     EFLKQHFGSTMEERSLVEEDVHFQLPPI"
     gene            complement(6257..6406)
                     /locus_tag="PKN92_08705"
     CDS             complement(6257..6406)
                     /locus_tag="PKN92_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48912.1"
                     /translation="MAHKNTREMAHPAPAQILPILDIVLRIFSLLELLVRLIGYFKPS
                     PSAEG"
     gene            complement(6448..6993)
                     /locus_tag="PKN92_08710"
     CDS             complement(6448..6993)
                     /locus_tag="PKN92_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNZ48913.1"
                     /translation="MLNKDNTALVVIDIQDVLMPACEKVTTSYLEQSLRMIQVVKALE
                     LPVLVTEQYPERLGETNRVILDALGSILRLPKIEFSCFANDAFREAAAQTGRKQLLLI
                     GMETHVCVMQTALEGLEQGYEVFIARDAVVSSRKYDYKAGLSRMIQAGVTALTVEMAI
                     FELLRKAGTPEFKRVLPIIKG"
     gene            complement(7075..7539)
                     /locus_tag="PKN92_08715"
     CDS             complement(7075..7539)
                     /locus_tag="PKN92_08715"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNZ48914.1"
                     /translation="MESHLEKGTDRTASEQLRVSGGSHGQRVKRGQRVAVQQGRYGTG
                     PAPYGYKRLENSSGSLAIDDREAEVVRLIFREYLRVKSTGKVVDLLHERNIFTRKGNH
                     WSRQAIAIIISNRTYRGRVSYGDIETEGKHEAIIDPATFYKANAIKEEKSRR"
     gene            complement(7837..8598)
                     /locus_tag="PKN92_08720"
     CDS             complement(7837..8598)
                     /locus_tag="PKN92_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNZ48915.1"
                     /translation="MTTPANPKEEGETLRIAKLMAQRGLCSRREAEALIARRWVSVNG
                     VVVEVPGTLVAPDAVIELHPEAGKWLDQAVTILLNKPVGIVSGQPEDGYKAAIEIISQ
                     ENQYRVNANEPLFQPSHLQGLAPAGRLDIDSHGLLVLTQDGRIARQLIGEDSTIDKEY
                     LVRVSGEVTKKALQLLNFGLQLDGKALRRARVKLLEPGLLQFILREGKKRQIRRMCML
                     VGLEAINLKRVRIGKIRLGSLPYGQWRFLEKGEHF"
     gene            complement(8595..10331)
                     /locus_tag="PKN92_08725"
     CDS             complement(8595..10331)
                     /locus_tag="PKN92_08725"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNZ48916.1"
                     /translation="MNEKGQENLASPVPARVWAAALLLCAVAWSFRTTSFMHAKELVL
                     VGALVIATPLQWHLRCLYTPKQRLLLPLWAGLLFWAVTGVFQAKVHGYLLESLVRWQL
                     YLTVVWLAVPAFSVPGGKELLYKSLIGSAWIVAVLALLQYAGALPFLFPIFPAYTQPA
                     YSVFGNQNLLGGYVALALVLLPLLALHFPGRTLSSFVYRATAWLTLLAAVLISGSRSA
                     WLAVFFGLAVFFMSFFRKMQRQGIPFCSKARRAGGVSVLVLSLVLILLGMPLLKERIE
                     ITFTGNDVGGPARLWFWAGAREMIRERPFLGVGLGNYTYWSPFYQGKVLWNPGGEGFF
                     RNTLHTDHAHSEPIEWLAETGVVGCLFWILFLCLVAARSNPAAPALLALAFFALLNNF
                     SHSPPHLLAFLLLALNPEEECTKDELPRKLWIFPAAALIIVCYLGIIAVPSVLQCAAE
                     DQMDAGVAPEKAFAAANAWPWQSPSLQESLAAALIQEGKYQEAKQCLEQAIKGLDSYA
                     VHRLLLYCARTEDATAVYRAARNCLYRCPDDSEVWELLMKNCPPEELAFWQSRYIHFL
                     PPVNNQEGDDAE"
     gene            10568..11215
                     /locus_tag="PKN92_08730"
     CDS             10568..11215
                     /locus_tag="PKN92_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNZ48917.1"
                     /translation="MKQALIFWGGWEGHEPRACVARFEAALEQHGFDLTILNSTEVLA
                     DEKRMRQFDLLVPCWTMGHLEAKEERGLLQAVQQGAGIAGWHGGMGDAFRGNTDYQFM
                     TGGQFVAHPGDITEYTVHISRPEDPLLDGIRDFRMHSERYYMHVDPSNEVLAHIIFPE
                     MEACPGSGAVKMPAVWKRLYGKGRVFYSSLGHVAADFDVYECFEIMKRGMIWASR"
     gene            11242..12345
                     /locus_tag="PKN92_08735"
     CDS             11242..12345
                     /locus_tag="PKN92_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48918.1"
                     /translation="MKTMKIGLIGCGNISDIYLRNLSTFPGLQVEACADLIPARAVEK
                     AAQYRIPRTCSTEELLQDSEIEMVVNLTLPRTHAAVAREILLAGKHVYNEKPLALTRQ
                     EGQELLELAAAKNLRIGCAPDTVLGAGIQTARALIDEGLIGTPLSAAASMQCHGHEHW
                     HPAPDFYYEPGGGPLFDMGPYYLSALVTLLGPAAAVTAMAAKGFDTRIIGSAPRKGET
                     IQVTTPTHLAGVIQFESGVLCNTTMSFDVWKSTQPHLEIHGTRGSLLIGDPNTFDAAV
                     SLFRAEAQDWTALPLRQGYSSHMRGLGVADMAQAVRSGNNHRCSGTIAFHVLDIMHAF
                     LDSSHKKSEQPLSSRCERPTPAVDLFSSRQMES"
     gene            12411..13124
                     /locus_tag="PKN92_08740"
     CDS             12411..13124
                     /locus_tag="PKN92_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ48919.1"
                     /translation="MQLLKNLYQIGGDLNGITWAGEDAGFRDANSYALDLGETIVLFD
                     CGCGDTLDQLFANMRYWGLDPGRIGWCFLTHPHLDHAGAAHLLKAKGVRLVAHPYTGA
                     AIAAGDERCCGYLYHKTFVPTQVDDTLGDGETMTVAGLPIRALHFPGHTLGCTAWQFT
                     HDGKRVIVSGDVIGTLNVGYFGWDGSIDFDKTKYIDSLLRFARIDFDVMLSGHGMSHF
                     YMPRRRVEECLNQALIQWR"
BASE COUNT         3190 a         3530 c         3376 g         3043 t
ORIGIN      
        1 ccgggatacg ggcggatctt cagtctacct ttaataccgc tattaccgaa cacgaagcca
       61 ccatcccgga aatcctcgaa tcgatgctga cttgtacatg gatacggaag agacacgtcc
      121 acaatcatct gcgaagcaaa gaataggaat actatgtcat cgccgttaat caggtcagcg
      181 aaggcgaacc cggcaacacc atcatagcgg tgttgaaaag aaaaaataca gacatagaca
      241 aggggcgcca gaaaatgaat gctcacggcc ggttcagccg catcttgcat ccgctaatca
      301 ttgtatcggc aggaatcctc gcgactctat tactaacagg atgcccggac tttgactatg
      361 gagaatcgga cgcatgtgtc tgcgctaatg atcctatcct cgttcttgcc ccgccttacg
      421 gtacacccat tacttccatt aaaaaggaat ggtcaccctt gctcataggc tatcccggcc
      481 agatcatcga gattgaagta tccggcggtg atgataggga ttacgtgttc aacaccgctg
      541 tcgaccctct tgatggttgt ggtggtggtt gtccgcctac gcaagactgc atggacagat
      601 ttggtaactc cagtcgagta tgcgttacca acataagcaa ccggaatatt ccttaccttt
      661 gggatgcgtc tggttgtgtg gaatccattg agccagaccc ggacacggac acgggtctct
      721 gtgacaaatg ctacgttaag ttgaagaaag agtacataga gacagcagca gagtttaccg
      781 tgtctgtgac tcgccacggc gcatctcaag aagaacttca atgcgaaaac tttgcctatg
      841 ccgagtggaa aggggtggtg ctacctcctc cacagtttac gatgtcaatc tcaacgagtc
      901 cgcagaccgc gatcctgttg ctgtcaggtc ctgatgcgga aggctctgcc tggttgcgag
      961 aagacaagtc acaaaacccg accgtgccac aagatacgct taagcaaaga agcagtgatc
     1021 cgagcgaccg atctgttggt gttgactatc gagtacatct ccaatacacg gatcccttgg
     1081 cacctgtaga ttatgattcc acgaactccc ttcattttgc ggcaatgaga actttggact
     1141 tgcttggtag agatattctc atttcaaacc atttgtactt cacgggaata ccggctcctc
     1201 ctgttggacc agtgttttcc ggcgtgcacg gattagccta cgaaggctac gatttcatta
     1261 tattgagttc ggtcgaagta gttgacgtta agcagacttt cgcacacgag attgggcact
     1321 gctgtggtct ggctgacaac cagccgcttg tttctcacaa ccttatgctt agtggccttg
     1381 gtccaagcgg tttcacctat ggcatggacg tgcattcaca gttctcacaa aacttaaaac
     1441 ttgacgatgg ctcggaggac tggcacgact gaccctggcg ggcagtcatc ctctccaaga
     1501 gcgttatccg ataagaacag ctttcccttg aaccagaaag gaaaaggcaa tgaacaagaa
     1561 cgcaaaatac tctcagaaga tagcaggggg tgcgaccttg ccacacagat ggtcgatcag
     1621 aaaggtcctc tacgacatgg ctgttatcag cagcatgact gttatcatct tctgcgtgct
     1681 gatcgtcctg ttgctgtctc caatggtact gtgccaagtg gagcccggtg gcgtatttct
     1741 atatccctcg tcttcccccg acgttcccag gcctcatgac ccggacctca gtagttttga
     1801 tgatcccttc ctgaaggaaa tctacgaaag gacaatgatc ggcatccgca ctgcggagat
     1861 tcatcctgac gggccggcag ctagagtcca ggcaatcgct gatttctgtg ctgtacccaa
     1921 gccaccagag gagattgact atattgtggc ggcagcacgt gaagccgtgg aattgcaaca
     1981 atggagccgg taccgggcgg cactctggtg cacccaggca ggcgtagaga tcgaagtgcg
     2041 tctggtggct ttgtcccggg agatgcttca agcgccacgg ggcgaaaaac tttccgatga
     2101 gcacggattg gcaatatatg ataccctgga atttctggga cgtaccccga ttgaatcggc
     2161 ggcggattta ctcatggagg caaccaccct ggaattctgg ggaccgggcc cgcttcggtc
     2221 acgcaggctt cgcccggata cacaacaaag cgtaaccatg cttcgctcgc aggccgtcag
     2281 gaatctcggg aagctgccat cctctctttc cctgccccgg ctggaggagc tggcgaaaca
     2341 gtatccggat aaatccgcta catccgcacc cgtagaggag tatcgctttg aaatgggtgc
     2401 cggttatacc atcgccgagc agatttataa tgtgaaagtt cgggaaggaa tacctggtgc
     2461 catgcacccc tttgtcgaac tctacagaga gccttcggaa cctatggtag aacccgtgct
     2521 ttaagggtat gaactccagt gcacttttca gaagcgcttg tctttgacgg tggacaaaga
     2581 agaaagggat tgagatgtat aacgattatt tcagcgcatt ccacctgggg ccatgccggt
     2641 ggggtgtcag cctggtactt cttgtggttc tacttgcatt ttcggcggca ggcacagcgg
     2701 aacagctttt actgtgcaac ttcaacccgg ctccggaatt gccctatgag agcacctctt
     2761 tcgagcagga cgcgctctac gctgaggcga aacaacttga caagaaggat caggcgcttg
     2821 cagcgtgcag agccttgcgc caggattcat cggcgccctg ggaattgcag cgatgggcca
     2881 ctcttcgtga aatcgagatc ttgaccgtca tggatcagga gcacgccgct ttggatcttg
     2941 gtaaggcctg gttgcgccaa aacccagatg atcccggtgc gctcgaaatt cgggtggagc
     3001 tgggaaagat tgttgctcaa cggaggcaca gtggcttcat gccgactctt caagaagttg
     3061 acgagatctt cacagacatc ttcaacaatc atgatccgga ttcttggcat gttgtcacgg
     3121 cccacattca atacgctcaa aagctccaaa agttctactt ctccgatcgg gattggacag
     3181 tatacgcacg atgcatcgag caattgtata tcgcccgcca atgcctggag aaacgtgcaa
     3241 aagacgagac cctttcagcc caggaacgaa ggcgtgcgga atcgtacctg caagatcatg
     3301 ttctcccctg gttgtctcgt gccctgggat tcggcattaa agggagacca agggaactca
     3361 gcccggaaga aaaggaatta atccgccacc tggaggaaga aggaacgcat atacggcgta
     3421 gtgtgccaga agaggaatag aaaagctggt gtgttcgcgc cgtcagttct cacggtttcc
     3481 tgctttgtga gaaacaacag ataaagggaa ctgaattttc attccatctt gaaagaaagc
     3541 cgtttttcta gattttagaa agtttgagct cgtgcagatc gcgctcgagg acaaatacag
     3601 ggacggacta cgaaaaaagc aagaaatatt tttttagtag tctgtcccga tatttatttt
     3661 tggtcttttc cggcaatcat tttgaccggg agtaaggctt caaggtacac tatttatatt
     3721 cttcttttca taaaaccctc tcccagaatt aatacgagta cgcaccatga ataaaaatct
     3781 gcttatcggc tttgctgttc tgcttgtcgt ttttcttgtg ctgcctattg ccgcatcctt
     3841 cttgcggggc ggcagttccg gttccgctgc cccggttatt tccgcagaag aggcagggcc
     3901 tcccaagtgg aattcggaaa atcttgtcgg cacagcctgg agtgtaaaaa cacctgatat
     3961 ccctgttgca gtcactatca atcttgcagc gggcgggcag gccatagcga cagctcccgc
     4021 cattctcgcg cccatggtca aagcgcacct cggcaccgat accatcgtag gcacctggcg
     4081 tgttgaaggc gcaaaggtca tcgccagtgt gaatctgaaa gggaaggaag tctctgttgc
     4141 ctgtgatatt cgaggcgacc gtcttttcta tcaggataaa gaaattaaac gggtgggata
     4201 acaggcaggc ttcgaagaaa aggatttcat gacagcaaca gctctcgctc cgctggaaaa
     4261 agaagcaaaa ctgctggaac agctgcgccg ctatccttca gccgccctcg cctattcggg
     4321 cggcgtagac tccacttatc tcgctgatgt ggcacatgaa gcgctgggcg aaaatctcct
     4381 tctcctcatc gctgaagcag caaccatgcc ccgggaagaa tatgatttcg ctgtcgctca
     4441 ggcgaaaaat cggggatggc ggctggaatc gttctctcct gctgtttttg atcaggtgtc
     4501 ctttgtccgg aactccgaca tgcgctgcta ctattgcaaa tctgaagttt ttgctgtaat
     4561 ggcacagcat gcggccgcac acggcatcac agtgctgctg catggcgaga cggcagaaga
     4621 tgcctttgac acaaccagag tcggcattcg ggctgcccgg gaagcagggg tacatgcccc
     4681 gcttgccgat cagggtttta ccaaagccga tattcgtgaa cgcagcagaa tcaggcagct
     4741 gcccacctgg gacaaggctt ccttcgcctg tctggcgacc cgcgtcccca ccggaacacc
     4801 tctctctacg gaactgctga accgtatcga aacggcggaa accgtactga aagattccgg
     4861 atttcatcag taccgtgcaa ggcatcacgg caacctctgc cgtatagaaa ttgagcctga
     4921 agaattcccg cgccttctcg atccggcact ccgccagcag atcattacag caatacaata
     4981 tgccggctac acctatgtta ctcttgatct gaaaggctac ggcaggcact gatcgcctgt
     5041 tggcaggtcg ctgccgtgtt tgttccgttc cggtgctttt tgcgccagat ggttgcatgg
     5101 ctgagtcgta acaaccacac cgcataaaaa acttttctga ggaaaatccg atatgcctca
     5161 ggctttgttt tttccggatt aaacgcccgc ctctgccggg caatgcggac taattgcttg
     5221 tttgcaggtg tttatgacag gtaccatgaa cgccacggca ggttccgtgg ttttctattt
     5281 tcttcagcca atcctctatc gccctcttta caatgaaaag gtctttccat gcatatcctt
     5341 cttgcaaaac cccggggctt ctgtgcgggt gttgaccgtg ccatcaaaat agtggaacac
     5401 gcactggaac gctatggtgc ccctgtttat gtgctgaagc acattgtgca caatgcgcat
     5461 gtagtggaga agctgcgccg tcagggagct gtctttgtga agacccttga ggaagtgccg
     5521 gaagaaagtc gcctgctttt cagtgcccac ggcgtctccc ctgaccgcca tgcagaagcg
     5581 aaagcccggc atttacacgt catcgatgcc acctgcccgc tggtggcaaa ggtacacaaa
     5641 gaaatccggc gttttgctga agacggatac accattattt acatcggtga tgccggtcat
     5701 gacgaaacac tgggcgctgt cggctgggcg cctgagcata tacgcctggt tcgcacggaa
     5761 gaagacgcct ggaaggtttc tgttgccgat ccggaacggg tcgcttacat tacccagaca
     5821 accttgagca ttgttgactg cgaggacgtg gcgaacgttc tccgtgcacg ttttccgaaa
     5881 atcaccgccc cgaaaaaacc ggatatctgc tacgcaacaa gcaaccggca aatggctgtt
     5941 ttgtcgcttg tcccggaagt ggacaaggta cttgtggttg gagatccgga aagtgccaat
     6001 tcctgccgcc tgcgggacat tgcgctgaag ttaggcaaac cgtcttatct gattcaggac
     6061 gcgtcgcgca ttgataaagc ctggctatgc ggggacgaca cgatcctcct aacatccggt
     6121 gcttctgccc cggatcatct gattcgcgag gtcatcgaat ttttgaaaca gcacttcggc
     6181 agcaccatgg aagaacggag tcttgtggaa gaagacgtcc attttcagct gccgcctatc
     6241 tgatccgcac tgcctcttat ccttctgcgg acggggacgg tttgaagtaa ccaatcagcc
     6301 gcacaagcag ttccagcaga ctgaaaatcc gcagcacaat atccagaatg ggaagaatct
     6361 gtgccggggc gggatgtgcc atctcacgag tgtttttgtg tgccatggac tggctttcct
     6421 gagtaaaagt tatttcaacc aaaaatatca gccttttata ataggcagaa cacgtttgaa
     6481 ttccggtgtc ccggctttgc gaagcagttc aaagattgcc atctcaacgg tcaaggccgt
     6541 tacgcctgcc tgaatcattc gggacaaccc tgctttataa tcatacttac ggctggacac
     6601 aacagcatca cgggcgatga aaacctcgta cccctgctcc aacccctcca gagctgtctg
     6661 catcacgcac acatgagtct ccattccgat cagaagaagc tgcttgcgcc ctgtctgcgc
     6721 tgccgcttcg cgaaaagcat cattggcaaa acagctgaac tctattttgg gcaaacgcag
     6781 gatgctgccc aatgcgtcca ggataacacg gttcgtttcg cccagccgtt ccggatactg
     6841 ctcggtcaca agaacaggaa gttccagcgc tttcaccacc tgtatcatgc gcagcgactg
     6901 ttccagatag gaggttgtca ccttttcaca ggcaggcatc agaacatcct gaatatcgat
     6961 cacaaccagc gcagtattgt ctttattgag catgaagaaa ttcctgatcc tttgtgttgt
     7021 gccctttaca gaaaaaagaa aaggcctttg ggacacacat tgggaatggc ccaatcagcg
     7081 gcggcttttt tcttctttaa tggcgtttgc tttataaaaa gtagcgggat cgataatggc
     7141 ttcgtgtttc ccttcggttt ctatatctcc ataagaaaca cgacccctgt atgtccggtt
     7201 ggaaataata atggctattg cctggcggga ccagtgattg cctttgcgcg tgaaaatatt
     7261 gcgttcatgg agcagatcaa ctacttttcc tgtgcttttt acgcgcagat attcgcggaa
     7321 gataagacgc accacttccg cttcacgatc atcaatggct aaagagccgc tgctgttttc
     7381 cagacgttta tacccgtaag gcgcaggccc tgtaccatag cgtccctgct gtacagcgac
     7441 acgctgaccg cgcttcacgc gctgaccgtg actcccgcct gacacacgca gttgttctga
     7501 tgctgtgcga tctgttcctt tttctaaatg agattccatg cctgcgttcc ttgcggaaac
     7561 tccaatttgc gcacgtccga agtgcatcag ccctgatttg tcgcgtgccc aacagcgaca
     7621 tggttattct acgccatatt cgcaaaaatg tcaagagtaa actgctttca aaaggcacat
     7681 gccaaagatc catagaatac aaatgctttt aattcaataa gttgcatgct tctgccgcct
     7741 ttctcatctg cagatacgcc aaagtgctag aacgtactaa agtgcgataa aggaaacagc
     7801 agccccgttt tcttcgtaga acgccctccc accttttcag aaatgctctc ctttttcaag
     7861 aaaacgccac tgtccgtaag gcagtgaccc caggcggatt ttcccgatac gtacccgctt
     7921 caaattgatt gcctccagac cgaccagcat gcacatgcgc cgtatctgtc ttttcttccc
     7981 ttctctcaat atgaactgaa gcagtccggg ctccagcagt tttaccctcg cccgtcgcaa
     8041 cgctttgcca tccaactgca gcccgaagtt cagcagctgg agggcttttt tggtcacttc
     8101 tcctgaaacc cgcaccagat actccttgtc aatagtcgag tcttcgccga tgagttgccg
     8161 ggctatcctg ccgtcctgag tcagaacaag gagcccgtgg gaatcaatgt ccagtcggcc
     8221 ggccggcgcc agcccctgta aatgtgacgg ctgaaacagg ggctcattcg cgtttaccct
     8281 gtactggttc tcctgactga ttatttcaat ggcggcctta tacccgtctt cgggctgtcc
     8341 ggatacaatg ccgaccggtt tgttcagaag gatggtcaca gcctgatcca gccatttccc
     8401 tgcctccgga tggagttcaa taacggcatc cggcgccacg agggttcccg gcacttccac
     8461 caccacgcca ttaacggaaa cccatcggcg tgcgattaat gcctctgctt cccggcggga
     8521 acacagtccc cgctgcgcca tgagtttggc gatgcgcagt gtttcgcctt cttcttttgg
     8581 gtttgcaggt gtcgtcactc agcgtcatct ccttcctggt tatttacagg cggcaaaaag
     8641 tggatatacc gggattgcca gaaagcaagt tcttcaggcg gacaattttt cataagaagc
     8701 tcccatacct cactgtcatc ggggcagcga taaagacaat tgcgtgctgc acggtacacc
     8761 gctgtggcgt cctccgtccg ggcacaatac aaaagcaggc ggtgcaccgc ataactgtcc
     8821 agccccttta ttgcctgttc gaggcattgt tttgcctcct gatattttcc ttcctgaatc
     8881 agtgctgccg ccagactttc ctgcagggaa ggactctgcc agggccaggc attggcagcg
     8941 gcaaaggctt tttccggcgc cactccggca tccatctgat cttcagcggc gcattgcagc
     9001 accgagggca ctgcaataat tcccaaatag cagactatta tgagggcggc tgccggaaaa
     9061 atccataact ttcggggaag ttcgtctttt gtgcattcct cttccgggtt cagggcaagg
     9121 agaagaaaag ccagcaggtg tggcgggcta tgcgaaaaat tgttcagcag tgcaaaaaaa
     9181 gcgagcgcaa ggagcgccgg agctgccggg ttgctccgcg cggccacaag acacaaaaac
     9241 agaatccaga acagacagcc gacaacgcct gtttcggcaa gccattctat aggctcggaa
     9301 tgtgcatgat ctgtatgcag tgtgttccgg aaaaagcctt cacctcccgg gttccacagc
     9361 actttcccct gataaaaagg actccagtac gtgtagttgc ccagccctac cccgagaaac
     9421 gggcgttccc gaatcatttc ccgtgcacct gcccagaacc agagccgcgc aggtcctccc
     9481 acatcattgc cagtaaaggt tatttctatc cgctctttaa gaagcggcat gccaagcagt
     9541 atcagcacaa gcgaaaggac caggacagat acccctcccg ctctcctggc ttttgaacaa
     9601 aaagggatgc cctgccgctg catttttctg aaaaaggaca taaaaaaaac ggccagcccg
     9661 aaaaatacgg ctagccaggc agaccgggac cctgagatca gcactgccgc caaaagcgtg
     9721 agccaagccg tagcacgata cacaaaagaa gaaagcgtac gtccgggaaa atgcaatgcc
     9781 agaagaggca gcagaaccag ggcaagcgcc acatacccgc ccagcagatt ctgatttcca
     9841 aaaacggaat aggcaggctg tgtataggca ggaaaaatgg gaaacagaaa cgggagagcg
     9901 cctgcatact gcaacagggc aagtaccgcc acaatccagg cggatccgat cagactttta
     9961 tacagcagct ctttgccgcc cggcaccgaa aaagccggaa ctgcaagcca tacaacagta
    10021 agatacagct gccagcgcac caggctttca agaagatatc catgtacttt tgcctgaaat
    10081 acgcctgtga ccgcccagaa gagcagcccc gcccataacg gaagcagcaa tcgctgcttc
    10141 ggtgtgtaaa ggcatcggag gtgccactga aggggcgtgg caataaccag cgcaccgaca
    10201 agcaccaatt ctttggcatg cataaacgaa gtggtgcgaa aagaccaggc aacagcgcaa
    10261 agcagcaatg ccgccgccca gacccgggca ggcaccggag acgctaaatt ttcctgtcct
    10321 ttttcgttca tgtaccgtct ttcagcccgg atctgtgtca cagtggaaca atgtgtttcg
    10381 cccccggatt atcataacat accgggcatt gctttccggg agtttccctg cccgcccggc
    10441 tgaacggaac aggaatcact tcggacatgc aaccgttttg agattcctga ttcatcgcct
    10501 ggttccggta tacttggata tcttttctgc acacagcgtt taaatcaaca tcaaggaaag
    10561 cgtctctatg aaacaggctt tgatcttctg gggcggatgg gaaggacacg aaccacgcgc
    10621 ctgcgtggcg cgttttgaag ctgctctgga acagcatgga tttgacctga ccattctcaa
    10681 ttcgactgaa gtcctggccg acgaaaaaag aatgaggcag tttgacctgc tggtaccgtg
    10741 ctggacgatg ggccacctgg aagcaaaaga agagcgtggc cttctgcagg cggttcagca
    10801 gggcgcaggt atcgccggct ggcatggcgg catgggagac gcctttcgcg gcaatacgga
    10861 ttaccagttc atgacaggcg gtcagtttgt cgcccatccc ggggatatca cggagtacac
    10921 cgtgcatatc tcccgcccgg aagatccgct tcttgacggt atccgggatt tcagaatgca
    10981 ttctgaaagg tactacatgc atgtggaccc atccaacgag gttcttgcgc atattatttt
    11041 tccggagatg gaagcctgtc caggatcggg tgcggtcaaa atgcctgccg tgtggaagcg
    11101 gctgtacgga aaagggcgcg ttttttactc atcactgggt catgttgcgg ctgattttga
    11161 tgtatatgaa tgttttgaaa tcatgaagcg cggcatgatc tgggcaagcc gctagcgttt
    11221 ttcaaccaaa aggtactcac catgaaaacc atgaaaatcg gcctaatagg ctgcggcaat
    11281 atctcagaca tatacctgcg caatctcagc actttccccg gactgcaggt agaagcatgt
    11341 gccgacctga tacctgccag agccgttgaa aaagccgcac aataccgtat tccacggacg
    11401 tgcagcacag aggaactgct gcaggattcc gagatagaaa tggtagtcaa tcttaccctg
    11461 ccccggactc acgccgccgt tgcccgtgaa attctgctgg cgggcaagca tgtgtacaac
    11521 gaaaagccac tggcactgac acgccaggaa gggcaggaat tgcttgaact tgcggcggca
    11581 aaaaatctcc gcatcggctg tgctccagat acagttctcg gagcaggcat acagacagcc
    11641 cgtgctctga ttgatgaagg gcttatagga acacctctgt ccgctgcagc ctccatgcaa
    11701 tgccacggcc atgaacactg gcatccggca ccggactttt attatgagcc cgggggaggc
    11761 ccgctttttg atatgggccc ttattacctg tctgcactgg taacgctgct gggaccggct
    11821 gcagccgtaa cagcaatggc cgcaaaaggg tttgacacca gaatcatcgg cagcgcaccc
    11881 cgaaaaggcg aaacaatcca ggtgacaacc ccgacgcatc ttgccggggt tattcagttc
    11941 gaatcggggg tgctctgtaa cacgaccatg agctttgatg tgtggaaaag cacccagccg
    12001 cacctggaga tccacggaac ccggggctct ctgcttattg gcgaccccaa cacatttgat
    12061 gccgccgttt cgcttttcag ggcagaggca caggactgga cggcgctgcc gctccggcag
    12121 ggctattcga gtcatatgcg agggctgggc gttgccgata tggcgcaggc tgtgcgcagc
    12181 ggaaacaatc accgctgcag cggaaccatc gcttttcatg ttctggatat aatgcatgct
    12241 tttttagaca gcagccacaa gaaatccgaa cagcctctgt ccagtcgatg cgaaagaccg
    12301 actccggcag tcgatctctt ttccagcaga cagatggaaa gctgaagcga aaagccttta
    12361 tactcaatct gccgcactga accggaaaaa caaaaaaaca ggacacggat atgcagctac
    12421 tgaaaaatct ttatcagatt ggcggagatc tcaacgggat tacctgggct ggagaagatg
    12481 ccgggttccg ggatgcaaac agttacgcac tggatcttgg agaaaccatt gtcctttttg
    12541 actgcggatg cggcgatacc ctcgatcagc tgtttgcgaa tatgcgctac tgggggcttg
    12601 atccggggag aatcggctgg tgctttctga cccatccgca tctggatcat gcaggcgccg
    12661 cccatctgct gaaagcaaaa ggcgtccgcc tcgtcgcaca cccgtatacg ggcgctgcca
    12721 tcgcagcagg tgacgaacgc tgctgcggtt atctgtacca caagactttt gtgccgacac
    12781 aggtcgatga caccctcgga gacggcgaaa ccatgaccgt tgcgggactg ccaatccggg
    12841 cactgcactt tccggggcat acgctgggct gcactgcctg gcaatttacc catgacggga
    12901 aacgggtcat tgtcagcggt gatgtcatcg ggacgctgaa cgtgggttac ttcggctggg
    12961 acggctcaat tgattttgat aaaacaaaat atatcgactc cctgcttcgc tttgcccgca
    13021 ttgactttga tgtgatgctg tccggtcacg gcatgagtca tttttatatg ccccgtcgtc
    13081 gtgtggaaga atgtctcaat caggcattga tccaatggcg ctgagttttt gggctgccc
//
LOCUS       DAOPWB010000085        12989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4893_length_12989_cov_5.661899, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000085 DAOPWB010000000
VERSION     DAOPWB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4893_length_12989_cov_5.661899"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..2269)
                     /gene="fusA"
                     /locus_tag="PKN92_08745"
     CDS             complement(173..2269)
                     /gene="fusA"
                     /locus_tag="PKN92_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ48920.1"
                     /translation="MPRKSSIDKIRNIGIMAHIDAGKTTVTERLLYYSGRLRRMGEVH
                     DGAATMDYMEQERERGITITSAATACEWKNHHINIIDTPGHIDFTAEVERCLRVLDGA
                     VAVFCAVAGVQSQSETVWRQAQRYRVPRIAFINKMDRVGADFYRAVSTMTARLDAHPL
                     PLQMPVGAEEKFRAVIDLVEMRMISWNCENPDADQIISEVPADLLVEAEIRRREMIEA
                     IASEDDQLMESFLEDEQGITKAEIKAAIRRCTLASKLTPVFTGTALRNKGTRLLLDAI
                     VDYLPSPLDMGEYEGTKPRQPGRVVTRKPDDEEPFSALAFKILTDQHVGRLTFVRVYS
                     GKAKAGQQMLNARTERKERMGRFLQMHADERTELEEIFAGDIAAIVGCKDTTTGDTLC
                     DARKTIALMSVDFPEPVVHVAIEAKSKAEQEKLSKALSKLSDEDPTFQVRVNEETGQT
                     IIAGMGELHLEIITDRMRREFGVQGNVGKPMVAYRETLAGSAEVEKRFVRQTGGRGQF
                     AHVVLTLEKGETGSGFVFEDKTVGGVIPKEYIPAIEKGCRQAAESGIVTGYAMVDVKV
                     TLTFGSYHEVDSSDMAFQIAGSMAVKEAAPKAQPVLLEPIMKIEVNTPGDYTGDVIGD
                     FDRRRGRLIGMENDGSTQLIHAMVPLAEMFGYANDLRSKTQGRATYSMEFAQYEAVPR
                     SIANEVMEKMGSGYRF"
     gene            complement(2515..2988)
                     /gene="rpsG"
                     /locus_tag="PKN92_08750"
     CDS             complement(2515..2988)
                     /gene="rpsG"
                     /locus_tag="PKN92_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNZ48921.1"
                     /translation="MVRRREVIKRTPQPDPKYGSVTVAKFVNAVMKNGKKSVAESIVY
                     GAFDILKGKVTDQDPLSVFEAALENAKPLLHVKSRRVGGATYQVPVEIAPATRTAIAF
                     RWIIEFSRKRGEKTMKERLAGELLDCYTKQGSTIKRKEDTHRMAEANKAFAHFRF"
     gene            complement(3043..3465)
                     /gene="rpsL"
                     /locus_tag="PKN92_08755"
     CDS             complement(3043..3465)
                     /gene="rpsL"
                     /locus_tag="PKN92_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNZ48922.1"
                     /translation="MPTINQLVRNCRKKIVRKTKSPALKGCPQASGTCTRVATLTPKK
                     PNSAMRKIARVRLKNGLEVTAYIPGVGHNLQEHSQVMIRGGRVKDLPGVRYHLIRGVL
                     DATGDLGGTASVKDEGGKKVHKGRWVSRSKYGVKRPKK"
     gene            complement(3794..4738)
                     /locus_tag="PKN92_08760"
     CDS             complement(3794..4738)
                     /locus_tag="PKN92_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ48923.1"
                     /translation="MKKKTIVICAGLLLIICLFAVGQGWGRFLYLYAMPSAGGAYFDS
                     DGVRIHYREEGNGSPLILVHGLGVNSDINFQARGVTGRLAKEFHLILPDLRGHGRSDK
                     PHDVEAYGVEMCKDIIRLMDHLHIDKAQVLGYSLGGFVVLKLVSLYPERFVSYAPCGA
                     GWTPDSEKELSFAFELASEIEAGKGYGALSDYLTPIGGKVSKQKRTMMSLGLRMINDE
                     QAIAAVLRSMPALAVSEEELTGIELPGLAVIGERDPFRVFTQKLADALPSIRMEIIPG
                     TDHMSTLKSPDAQEVIASFFKAQEGALFQEPAQHEPAR"
     gene            4965..6257
                     /locus_tag="PKN92_08765"
     CDS             4965..6257
                     /locus_tag="PKN92_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48924.1"
                     /translation="MPNLLNRRSFIQQSSVIVSGALLSTAKAAPSERIRLGLIGTANR
                     GGQLFDAVTPHSDAEITAFCDVHGSALQKWREAYPDAIFESDYRRLLDRDDIDAVIVA
                     TPDHWHALQTIHACQAGKDVYCEKPLSLKLHEGRLMLDTARQHNRVVQVGLQRRASTM
                     YQQLAEDLHNGLIGKITSAHCYRVTNMTPNGIGKMAPSDPPADLDWDAWLGPRPMRPY
                     QANIAPYKFRWWKEYSSQVANWGLHFIDVLRWLMNEEAPRAVMALGGHYAVDDDRTIP
                     DTSVITYEMPGGALIVYEEYEANGTRPFPITSDIMIRGTKGTLFASDRAYEIVPEKGG
                     QFQDNEARMEGIKKKAEDGSHPAALMRNFLDCIKSRQRPIMDIEEGHRSSIFAHLANI
                     ALETRSMIEWDSAAERITNCPQANDLLMYEYRAPWTLG"
     gene            complement(6286..7548)
                     /locus_tag="PKN92_08770"
     CDS             complement(6286..7548)
                     /locus_tag="PKN92_08770"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNZ48925.1"
                     /translation="MTSPVFLSPEQAFTLRAQYGTPLYIYDEKTLSAQADQVLSFPNA
                     FGLTGRYAMKALPSAAILRLFHRKGLHIDASSGFEVERAWKAGIPSANIMLTAQEIPS
                     NLKELVNSGCLYNACSLHQLEIFGQLFPGREVSIRINPGLGSGHSNRTNVGGPSSSFG
                     IWHEHTDRALAIARKHNVKITMMHTHFGSGADPEVWMNCARLALRIAAKLPDVQRLSL
                     GGGFKVARVESEKGADLQEIGKHLLPAFQQFAQEQGRRLHLEIEPGTFLVAHGGALLV
                     SVIDIVDTGDGGYRFIKTDGGMTEILRPSLYGAQHPITLMPSTERAKEKADYLVVGHC
                     CESGDILTPEPGNPEGLLPRSFVRPEIGDLLFVGGAGAYCSGMPAKNYNSFPEAAEVL
                     INASGKATLIRRRQTLDQMIQNEIAEDE"
     gene            complement(7736..8215)
                     /locus_tag="PKN92_08775"
     CDS             complement(7736..8215)
                     /locus_tag="PKN92_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48926.1"
                     /translation="MTKPEQPNPESSLSFESLRAELGDHPFSVPSEEEHRTPAESEET
                     PLFQEQGRAERTSPKPEIRMEALTGSSPKHRPVWAEALFCIFFSCLLLLTLIMKSGFV
                     SYQPWISRIAILLALGGAGWASLGLRTAESKGGKRFIWGIVAGGLIIAVLAYLRMPQ"
     gene            complement(8208..9311)
                     /locus_tag="PKN92_08780"
     CDS             complement(8208..9311)
                     /locus_tag="PKN92_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ48927.1"
                     /translation="MASVVLGRYLGRPPRCLPVLLLFLTDRCNLRCRMCGVCERDQGE
                     AYKNELSSDEWRSVIAAAADHLGTFMMSISGGEAMIRQDLCEIISFAVSKGIHVHLCS
                     NGFLFTEEKVDALRDAGVSMISISVDGPDAETHDFLRGKKSFEAAVRAIRLVRERAPE
                     VFLGINFLIAPHNYLRMQEMVSFAKAEGIVKLKFAPLHTNLLHRYKSQQDFDGLFFKA
                     EELPLLEAELQRTRKACAAQGIATTSDGFFKGIPLFYQGKQSFRCYAGYAVCAISPYG
                     EVMPCCDHDTGLSVKDRPLNEIWHDPVFHEARKRVHHCKSACWDTTNTELSLRLRPET
                     VPANALQILRDLRFYFGKKSKTPINKESVTDSD"
     gene            complement(9439..10212)
                     /locus_tag="PKN92_08785"
     CDS             complement(9439..10212)
                     /locus_tag="PKN92_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48928.1"
                     /translation="MARRKKKTEASEYFLAPEEPKTEWQKFLVHLEENIKLYVAGTLF
                     IILCLAIGGLLRMSAAVKDKNIMTEYAEIVMEEDAGKRLAKYEELSPKAGRWTAEVLY
                     RQGETALEIGDFSAAKEAFNAVLKDHGKSEFAPSALDGLAFALNEEGKLQEALDAYDR
                     LTQEWPSAFVSRRAFLAKGALLEKLDRPAEAVAAYQHQRDLFPDSSSGRNAETAMNTL
                     LEKHPELKPAESADAGMPSPEESVSTEVLSTSNNESAAE"
     gene            complement(10305..10778)
                     /locus_tag="PKN92_08790"
     CDS             complement(10305..10778)
                     /locus_tag="PKN92_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48929.1"
                     /translation="MNYKALRFSLLLCLIGLYGCQTQAPFDLGRLVNPRMSDEEKITM
                     ILEDVQRGMEAQRVYQVLSHVSRAYKDRDGRDFSALQEDLNTLMKNYRNIRITRTPPR
                     IEVRGNRARVTDTFGTIAEPVRADDFPPVNLQGQVILMMEKTGDTWQIIEWGPIT"
     gene            complement(10782..11306)
                     /locus_tag="PKN92_08795"
     CDS             complement(10782..11306)
                     /locus_tag="PKN92_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48930.1"
                     /translation="MTRYIFSALFVCTLLLAGCQSMPWQRNTMPPVELLDDESGKSAP
                     EASAPPAASLQPAPKQAPVLAIASRTRMRDVPLPEKAKEDLERTYVYESHTLQVGRMV
                     YTIQAAVNDIAQFYIDTCPGADWRMVSVKQAAGGAELLFRKPGKKLEVSISPLAFGNK
                     RLVVHLVPDEGLNY"
     gene            11782..12588
                     /locus_tag="PKN92_08800"
     CDS             11782..12588
                     /locus_tag="PKN92_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48931.1"
                     /translation="MKTHKQPFILFLAIALTLGASFALAQNPPASDALPGQAPPELMD
                     SPKAPLPPARGFDQLDVNKDGAVSREEFSSNWLPCSPNRFARLDVNNDGVLSKDELAN
                     CPVCGGIPQGGRGLRAGQGPMGPGGGYCMAPGQGRAAGRGAYGMGPRQGRAAGRGAYG
                     MGPRQGRAAGRGAYGMGPGQGRAAGRGAYGMEPGQGGATGMGRSGMNRPALRPLLQNV
                     DTNQDQKITQEEYEKAWQDFHKEQFDLLDTNKDGSLTADELPPRFAPRTR"
     gene            12723..>12989
                     /locus_tag="PKN92_08805"
     CDS             12723..>12989
                     /locus_tag="PKN92_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48932.1"
                     /translation="MRNHLLTAFLILFGLLIGAGQYLEHRSMALHAEAALEHRAQDLA
                     AAFSVVIRSQGRGAHMSRDRLEDALIDLVHTASLESVVLLDESGN"
BASE COUNT         3134 a         3534 c         3210 g         3111 t
ORIGIN      
        1 tcgttcccac attcacatgc ggcttggtac gcgaaaattt ttccttcgcc ataacggctc
       61 tctccttagt gcggcgctaa gggcgctcgc agttctgcct tcaccgggat ttcagacgtt
      121 aaaccggaac taaggccgac atcatgtatt ttcctgatcc ggcaggggcg ctttaaaagc
      181 gatatccgct gcccatcttt tccataactt cgttggcgat tgaccgtggc accgcctcat
      241 attgggcgaa ttccatgctg taggttgcgc gcccctgcgt tttggaacgc aggtcattgg
      301 catacccgaa catctccgcc aaaggtacca tggcatgaat caactgggtt gatccgtcgt
      361 tttccatgcc aatgagacgt ccccgtctcc ggtcaaaatc gcctataaca tcgcctgtat
      421 aatctccagg cgtatttact tctatcttca taattggctc aagaagaacg ggctgtgctt
      481 tgggtgccgc ttctttcacc gccatggagc ctgcaatctg aaaggccatg tcggaggaat
      541 ctacttcatg gtaggatccg aaagtaagtg ttactttcac atccaccatg gcgtagcccg
      601 tcacaattcc gctttcagca gcctgccggc atcctttttc tatggccggg atatattctt
      661 tgggaatgac accgccaact gttttgtctt caaatacaaa accgctgcct gtttctccct
      721 tttccagcgt aagcaccaca tgagcaaact gtccacggcc gcctgtctgc cggacaaagc
      781 gtttttccac ttctgccgat ccggccaggg tttcccgata agcaaccatc ggtttgccga
      841 cgttaccctg gacaccgaat tcccggcgca tacgatcggt gataatctcc agatgaagct
      901 cacccatgcc ggcaataatg gtttgccctg tttcttcgtt aacacgcacc tgaaaggtag
      961 gatcctcgtc ggaaagtttg ctgagcgctt tggaaagttt ttcctgctcg gctttacttt
     1021 ttgcttcaat tgccacatgc actaccggct cagggaaatc aacagacata agcgcaatgg
     1081 ttttccgcgc gtcacaaagc gtatcacctg ttgttgtatc cttgcagcct acaatggcgg
     1141 caatatctcc ggcgaagatt tcttccagtt ccgtccgttc atccgcatgc atctgcagaa
     1201 aacgccccat ccgttctttc cgttcagtac gggcattaag catctgctgt ccggctttcg
     1261 ctttaccgct gtagacccga acaaaagtga gtcttcccac gtgttgatct gtcaatattt
     1321 tgaaagccag tgcggaaaaa ggctcttcat catcgggctt gcgtgtgaca acccgtccgg
     1381 gctgccgcgg ctttgttcct tcatattccc ccatatccag cggggaagga aggtagtcga
     1441 cgatggcgtc aagaagaagt cttgtgcctt tattgcgtag ggcggttcct gtaaagaccg
     1501 gcgtaagttt tgatgccagt gtgcaacgcc tgatggctgc tttgatttcc gccttcgtga
     1561 taccctgctc atcttccaga aaggattcca tcagctgatc atcttccgaa gcaatggcct
     1621 cgatcatctc gcgccgcctg atttcagctt caacgagaag atctgccggg acttctgaaa
     1681 tgatctgatc tgcatccgga ttctcacaat tccaggagat cattctcatt tcaacgaggt
     1741 cgatgacggc gcgaaatttt tcttcggcac cgacgggcat ctggagagga agagggtggg
     1801 catcaagacg ggcagtcatc gtggagactg cgcgataaaa gtccgcaccc acacgatcca
     1861 ttttgttaat gaatgcgatg cgcggcacgc gatatcgctg cgcctgtctc cagactgttt
     1921 cggattggga ttgcactccc gccacagcac aaaagaccgc cactgctcca tcaagtacac
     1981 ggagacaacg ctccacctct gcggtgaaat caatatgacc cggagtgtca atgatattaa
     2041 tgtggtgatt tttccactca caggctgtgg cggcagaagt gatggttata ccgcgctccc
     2101 gctcctgctc catgtaatcc atggtagcgg caccgtcatg gacttcgccc atgcggcgca
     2161 aacggccgga gtagtacaag agccgttccg taacggtagt cttgccggcg tcaatatggg
     2221 ccataatgcc gatattccgg atcttgtcta tacttgattt acgtggcacc tgatactctc
     2281 ctgtgcagat cttctgccta caaagaaact ttagcgccgc atagataaag gattttttac
     2341 ttcctttcag acaaggctcc tgttcatcag aacttaggac cctacgctct gcctgattca
     2401 actgtcagaa tctacgacag aggcggcgcg ctcatcgctt ccgacgcagc gcgtcactct
     2461 ccattgtatg tgataatcaa ttccttcatg gataaatcca catcgaaccg ctctttagaa
     2521 gcggaagtgc gcaaaggcct tgttggcttc cgccatccga tgggtatctt ctttacgttt
     2581 aattgtcgat ccctgcttgg tatagcaatc cagcagttcg cctgccaggc gctctttcat
     2641 tgtcttctcg ccgcgcttgc gggaaaattc aatgatccag cggaaagcaa tggcggttct
     2701 cgtcgccggt gcgatttcga cgggcacctg ataggtggca ccgccgacac gacgggattt
     2761 aacatgcagc aggggcttgg cattttcaag agccgcttca aaaacgctca ggggatcctg
     2821 atcagtcact tttcctttaa gaatatcaaa ggccccgtag acgatgcttt cggcaacgct
     2881 tttcttgccg tttttcatga cagcgttaac aaattttgct actgtaacac tgccgtattt
     2941 aggatcgggc tgaggagtcc gtttgataac ttcacgacgt ctgaccatga tttcaacatg
     3001 cctttatctt tagccgccct aaagcgacac ggttgcttca gattactttt tgggacgttt
     3061 cacaccgtat ttggaacggc tgacccaacg tcctttatgt acctttttcc cgccttcgtc
     3121 tttcacgctt gccgtaccgc ccagatcacc ggtcgcatcc agcacaccgc ggatcaggtg
     3181 gtaacgcaca ccggggagat cttttacacg accgccacga atcatgacct gcgagtgttc
     3241 ctgaagattg tgtcccacac cgggaatgta tgccgttacc tcaaggccat ttttcagccg
     3301 gacacgggca attttccgca tagctgaatt cggcttcttg ggcgtaaggg ttgcaacacg
     3361 ggtgcatgta ccggacgctt gagggcagcc cttcagcgcc ggggactttg tcttcctcac
     3421 gatttttttg cggcaattac gtaccaattg attgatcgtc ggcaaaacac tatctccaaa
     3481 cacagttttt tcgggacgtt ttttctggaa aaaacctgcg caaaaacacc cgctatgcag
     3541 acgcacaggt cgagggatta cgtacaagga aacctattat aacaaaactg aaagtgccag
     3601 tcaagcaata tgcatatctt ttttctgcat caggatttcc ccccggagca gatgcaaaaa
     3661 acgcaggcac ttggtaagta taacccattt gcgcactgaa aagccacttt caaccctctt
     3721 ctcaatcagc agcggatttt accagcacag cgtaaaacaa gtttggcgaa aatactgttc
     3781 atatccaaga ggtttagcgg gccggctcat gctgcgcagg ttcctggaaa agggctcctt
     3841 cctgggcttt aaaaaaagaa gcaatgactt cctgcgcatc cgggcttttt aaagtggaca
     3901 tgtgatccgt gcccggaatt atctccattc tgatggaagg cagggcatca gccagcttct
     3961 gggtgaaaac acgaaaggga tcccgttctc caataacggc gaggccgggc aattcaattc
     4021 cggttaactc ctcttctgat accgcaagag cgggcatgct gcgcaagaca gccgctattg
     4081 cctgttcatc gtttatcatg cgcagcccca ggctcatcat tgtccgtttc tgttttgata
     4141 cttttccgcc aatgggcgtg agataatcag acagagcgcc gtaacccttt cctgcctcaa
     4201 tttctgaagc cagttcaaaa gcgaaagaaa gttctttctc actgtccggc gtccaccccg
     4261 ccccgcaagg cgcataagaa acaaaacgct ccggataaag actgacaagt ttcagcacca
     4321 caaatccacc cagagaatat cccagcacct gcgccttgtc aatgtgcaga tgatccatca
     4381 gccgaataat atctttgcac atttcaaccc cgtaagcctc cacgtcgtgc ggcttgtcac
     4441 tgcgtccgtg tccgcgcaag tcgggcagga tcaggtgaaa ctctttggca agccgcccgg
     4501 tgacaccacg ggcctgaaaa ttgatgtcac tgttaacacc cagtccgtgc acaagaatca
     4561 gcggacttcc gttcccctct tcgcgataat gaatccgcac accatcggag tcaaaatagg
     4621 cgcctcctgc agacggcatt gcataaagat aaaggaaacg cccccagccc tggccgactg
     4681 caaagaggca gatgatcagc agcagtcctg cacaaatgac aattgttttt ttcttcatcg
     4741 tctcaccctt ttcgctttcg ctgaccgaag gatctgagaa aacatgcaca gggcaagtgt
     4801 agcaggattt aaaaggatca atcattttta aaccccgccc gaagtttgtt catcgcatgc
     4861 aaacatgcta tcctcaaaat actgatgtcg atgcctgaat tacgacctgc aggcagcaga
     4921 aaacaaacct gtttaacggg acgctgatcc atggagaaaa gaacatgcca aatctattga
     4981 accgccgcag cttcatccag cagagctctg ttatagtgtc gggtgctttg ctctccaccg
     5041 cgaaagcggc accttctgaa cgcatccgcc tcgggcttat cggaacagcc aaccgcggcg
     5101 gacaactgtt tgatgcagtc actccgcaca gcgacgcgga gattacggct ttctgcgatg
     5161 tccatggcag tgctcttcaa aagtggagag aagcatatcc cgatgccatt ttcgaaagtg
     5221 attaccgccg tcttcttgat agagacgata ttgacgcagt cattgttgcg acccctgatc
     5281 actggcatgc ccttcagact atccatgcat gtcaggcagg aaaagacgtg tactgcgaaa
     5341 agcctctttc cctgaagctg catgaaggcc gcctgatgct ggacacagcc cgccagcaca
     5401 atcgtgttgt ccaggtaggg ctgcagcgac gcgcttccac catgtaccag cagctggctg
     5461 aagatctgca caatgggctc attggaaaaa tcacgtcggc gcactgctat cgtgtcacca
     5521 acatgacacc gaacggtata ggcaaaatgg cgccctccga ccctccggcg gatcttgact
     5581 gggatgcctg gctcggaccc cggccgatgc gcccctatca ggcaaatatt gcgccttata
     5641 aattccggtg gtggaaagag tattcttctc aggtggcgaa ctgggggctc catttcatcg
     5701 atgtgctgcg gtggctaatg aacgaggagg cgccccgtgc tgttatggcg ctgggcggac
     5761 attatgctgt tgacgatgac cgcaccatcc cggacacctc ggtcatcacc tatgaaatgc
     5821 ctggcggtgc tctgatcgtt tatgaagaat atgaggccaa cggcacccgc cccttcccca
     5881 tcacttccga tattatgatt cgcggcacca aagggacttt attcgcctca gacagagcct
     5941 acgagattgt tcctgaaaaa ggcgggcagt ttcaggataa cgaggcgcgc atggaaggga
     6001 tcaagaaaaa agcggaggac ggaagccacc ctgcagcgtt gatgcgtaat ttcctcgact
     6061 gcattaaaag ccgccagcgc cccatcatgg atattgagga agggcatcgc tcatctatct
     6121 tcgcccatct cgccaatatc gcactggaaa cacgctccat gattgagtgg gacagcgctg
     6181 ctgagcgcat cacaaactgc ccgcaggcaa atgacctgct catgtacgaa tatcgtgccc
     6241 cctggacact gggctgatcg gatcggacgc ttaaaaaacc gtcttttatt cgtcttcggc
     6301 aatttcattc tggatcattt gatccagtgt ttgcctgcgc cggataaggg tcgctttccc
     6361 cgacgcatta ataagcactt cagccgcttc cggaaagctg ttatagtttt tggcaggcat
     6421 accggaacaa taggcaccgg caccgcccac aaaaagcaaa tcgcctattt ccggccgaac
     6481 gaaagaacgg ggcagcagcc cttccggatt gcccggctcc ggcgtcagaa tatctccgct
     6541 ttcgcagcaa tgcccgacta ccagatagtc cgctttttcc ttcgctcttt cagtgctggg
     6601 catgagggtg atgggatgct gcgcaccata aagagagggc cggagaattt ctgtcatacc
     6661 gccatccgtt ttaataaacc tgtaccctcc gtcgcctgta tcaacaatat caatgaccga
     6721 tacaagaagc gcaccgccat gggcgacgag aaaggtgccc ggttcgattt ccagatgcag
     6781 ccgcctgccc tgttcctgag cgaactgctg aaaagcgggc aggaggtgtt ttccgatttc
     6841 ctgaaggtct gccccttttt cgctctccac acgggcgacc ttaaaaccgc cgcccaaact
     6901 taaacgctgc acatctggaa gcttcgctgc aatacgcaaa gcaaggcgtg cgcagttcat
     6961 ccatacctcg ggatcagctc cggaaccaaa atgcgtgtgc atcatggtta tttttacgtt
     7021 gtgcttccgg gcaatggcaa gcgcacggtc ggtatgctca tgccagatgc cgaagctgga
     7081 ggaaggaccg ccgacattgg tacggttgct gtgccctgaa cccagcccgg gattgatgcg
     7141 gatggacact tctcttccgg gaaataactg cccgaaaatt tccagctggt gcagtgaaca
     7201 ggcattgtaa aggcagccgc tgttcacaag ttcctttaaa tttgacggta tttcctgagc
     7261 agtcagcata atgttggctg atggaatccc ggctttccag gcacgttcca cctcaaatcc
     7321 gctgctggca tctatatgca gacctttacg atgaaaaagc cggaggattg ccgcagaggg
     7381 aagcgctttc atggcataac gcccagtcag accaaaggca ttggggaaag agagcacctg
     7441 atctgcctga gcactcagcg ttttttcatc atagatatat aatggggtgc cgtattgcgc
     7501 acgcaaggta aatgcctgtt caggggacaa gaaaacaggg gaagtcatga aggtactcct
     7561 atatattttt ttgggctgct gtcaggcggc gttattctca cacagattct ttcattcgcc
     7621 acgctgcgaa taggttcatc ccacccggtg tgaacccgat taagggtagc actttctttt
     7681 accgcattgc acacagcccc ggagcagtca ttcccggcag aagaaccgaa attgattact
     7741 gaggcatgcg caggtaagcc agtacggcga taatcagacc gccggcaaca ataccccaga
     7801 taaaccgttt cccgcctttg ctttcggctg tacgcagtcc gagagacgcc cagccggcac
     7861 cgcccagagc taaaagaatg gctatacgcg aaatccaggg ttgataagaa acaaatccgc
     7921 ttttcattat aagagtcaga agcagaagac aggaaaagaa aatacaaaaa agtgcctccg
     7981 cccagacagg ccgatgttta ggcgaggaac ctgtcaacgc ttccatacga atttccggct
     8041 tgggagaggt gcgttcagca cgcccctgct cctgaaaaag aggcgtctct tccgattccg
     8101 ccggtgtacg atgctcctct tcagagggaa cggaaaaagg atggtcaccc agttccgccc
     8161 tgaggctttc aaaacttaaa gaagactccg gattgggttg ttcaggctta gtcactgtct
     8221 gtcacgcttt ccttgtttat gggcgtttta ctttttttgc cgaaataaaa acgcaaatcc
     8281 cgtagaatct gcagggcatt tgccggtact gtttccgggc gcaaacgcaa actgagttct
     8341 gtattggttg tgtcccagca tgccgacttg caatgatgca cccgcttacg tgcttcatga
     8401 aatacgggat cgtgccatat ttcatttaaa ggccgatctt ttaccgatag tcctgtatca
     8461 tggtcacagc agggcatcac ttcgccatag gggctgattg cgcacaccgc atagccggca
     8521 taacagcgaa aggattgttt cccctgataa aacagcggga ttcctttaaa aaatccgtcg
     8581 gaagtagtag caatcccctg agcggcgcag gcttttctcg tgcgctgaag ctctgcttca
     8641 agcaggggta gttcttccgc tttaaaaaac aatccgtcaa aatcctgttg acttttatag
     8701 cgatgcagca gattggtgtg caacggtgca aactttaatt taacgatacc ttcggctttg
     8761 gcaaaagata ccatttcctg cattctcaga taattgtgcg gtgcaatcag gaagttaatc
     8821 cccaaaaaaa cttcgggcgc ccgttcacgc acaagccgga tggcgcgtac tgctgcctca
     8881 aaactctttt ttccccgcaa aaaatcatgg gtctcagcgt ccggtccgtc cactgaaatg
     8941 gagatcatgg acacaccggc gtcccggagc gcatccactt tttcttctgt gaaaagaaat
     9001 ccatttgaac ataagtggac atgaatgcct ttagacacgg caaaagaaat gatttcacag
     9061 agatcctggc gaatcatcgc ttctccgccg ctgatggaca tcataaatgt gcccagatga
     9121 tctgcagcgg cggcaattac agagcgccac tcatcagagg aaagttcatt tttatacgct
     9181 tccccctgat cccgttcgca gacaccgcac atacggcagc gcaaattgca gcggtctgta
     9241 aggaacagaa gcagtacggg cagacagcgg ggcggtcgtc ccagataacg tccaaggaca
     9301 acagaggcca tacagagcac ctttcccaaa gtcagtccga acatgggaat gaggccttct
     9361 tcttactgtt aaaactcaat agcgtatgga acgcagcgga atcgcagcca ttcagcgact
     9421 ctgccttata cgttgtcttt attccgctgc actttcattg ttcgaagttg acagcacttc
     9481 tgttgaaaca ctttcttcgg gggagggcat tccggcatcc gcagattccg cgggcttcag
     9541 ctccggatgc ttttcaagca gcgtattcat ggctgtttca gcattacgcc ctgaagagga
     9601 gtccgggaag agatcgcgct gatgctgata agcggcaact gcttcggcgg ggcggtccag
     9661 tttttccaga agagcgcctt tagccaaaaa tgcacgacgg gaaacaaaag cagacggcca
     9721 ttcctgtgtc aggcggtcgt aggcatcaag cgcctcctgc aattttcctt cctcgttcaa
     9781 agcgaaagca agtccatcca gagcagaggg tgcaaactcg ctttttccgt ggtctttgag
     9841 aacagcgtta aaggcttcct ttgcagcact gaagtcgccg atttccagag cggtctcgcc
     9901 ttgccgataa agcacctccg cagtccagcg tccggccttc gggctgagtt cctcgtattt
     9961 cgccaggcgc ttccccgcat cttcctccat gacgatctcg gcatactctg tcatgatatt
    10021 tttatctttg acagcagcgc tcatccgcag cagaccgcca atcgcaagac acaaaataat
    10081 aaaaagggtg ccggcgacat agagttttat attttcttcg agatggacaa gaaacttctg
    10141 ccactctgtt ttaggttctt ccggagccag aaaatactcg gatgcctctg tctttttctt
    10201 gcgtcgcgcc atgaatttgt ctttcgtttc tgttcagttg tttgcgatag tacacaccat
    10261 taattatgga gtaattcaat acaaaggatc agacatgggg cgggtcaggt aattggtccc
    10321 cactcaataa tctgccaggt atcccctgtc ttttccatca tcagaataac ctgcccctgc
    10381 aagttgacag ggggaaaatc atcggcacgc accggttccg caatggtgcc aaaggtatca
    10441 gtcacccgcg cccggttacc acgcacttca atgcggggcg gcgtccgggt aatcctgatg
    10501 ttcctgtagt ttttcatcag tgtattcagg tcttcctgaa gcgcagagaa atccctgccg
    10561 tcacggtctt tatatgcccg ggacacatgc gataaaacct gatagacccg ctgcgcttcc
    10621 atcccccgct gaacgtcctc aagaatcatg gtaatctttt cttcgtcact catacgggga
    10681 ttcaccagac gccccagatc aaaaggcgcc tgcgtctgac agccgtaaag acctatcaga
    10741 caaagaagca gcgaaaatcg taaggcttta taattcatga atcagtaatt caacccttca
    10801 tcaggcacca gatgcaccac aagacgcttg ttcccgaagg caagaggaga tatggaaact
    10861 tcaagttttt ttccgggctt gcggaacagc aactcggcac cgcctgccgc ctgtttcacg
    10921 ctcaccatcc gccagtcggc gcccgggcat gtgtcaatgt aaaactgcgc gatgtcattc
    10981 acggccgcct gaatcgtata caccatgcgc cccacctgaa gggtatgcga ttcataaaca
    11041 taagtgcgct ccagatcttc ttttgccttt tccggcaggg gcacatctct catgcgggtg
    11101 cgggatgcta ttgccaatac aggtgcttgt tttggcgctg gctgcaggga tgctgcaggc
    11161 ggagcagatg cttccggcgc actcttgccg gactcatcat caagtaattc gacggggggc
    11221 atggtattgc gctgccaggg catgctctga cagcccgcaa gaaggagcgt gcagacaaaa
    11281 agagccgaaa aaatatatct ggtcatgggt acctcctgat cttttgtcaa gaacaccgaa
    11341 ttccagcgaa aggtgctttt tcgcaggcgc agggaaaaaa acacacacta ctttcccctg
    11401 cggttcaatt atagcataga aaattatcgg tactgctttc aacctgatac ttctgtaatg
    11461 ctttctaaaa atggaaaaca gcacccggca ttgttcaaat agaaattaaa tagacagaag
    11521 aaaaaaagca aaagggatcc cgggcgttaa acatggttcg agcaggggcg gcagggcggt
    11581 ctgcgtgctt tcgataaaaa ctgtgcatta tctgcacagc tttctgcctg ttgctgcgca
    11641 atttctgcac acgaaggagg cgaggcagcc gaaaatgggt gaattgctgc cgtttttagg
    11701 ctggcatgga gtttgctcta taaaagctga tgtaaatagg cagttgaaaa gaaccctcaa
    11761 acaaatgaaa aggagcctct catgaaaaca cacaagcaac cattcatcct cttccttgcc
    11821 attgcactga cgctcggcgc gtcctttgcc ctggcacaga atcctcctgc ctccgatgca
    11881 cttcccggtc aggcaccgcc ggaactgatg gattcgccta aggcccccct gccacctgca
    11941 cggggctttg atcaactcga tgtcaataag gatggtgcag tcagccggga agaattttct
    12001 tccaattggc tgccctgttc tcccaaccgg tttgcccggc tggatgtcaa caatgacgga
    12061 gtgttaagta aagacgaact tgcgaactgt cccgtatgcg gcggtatccc gcaaggcggg
    12121 cgtgggttga gagccggcca gggacctatg ggcccgggag gcggttattg catggcaccc
    12181 ggccaaggaa gagcggcggg cagaggcgct tatggcatgg ggccccgaca gggaagagcg
    12241 gcgggcaggg gcgcttatgg catgggaccc cgacagggaa gagctgcggg cagaggcgct
    12301 tatggcatgg gacccggaca gggaagagcg gcgggcagag gcgcttatgg catggaaccc
    12361 ggccagggcg gagccaccgg catgggcaga tctggtatga accgtcctgc cttgcgtcct
    12421 cttcttcaga atgtggatac caatcaggac cagaaaataa cgcaggaaga atacgaaaaa
    12481 gcctggcagg actttcataa ggagcagttt gacctcttag acaccaacaa agatggaagc
    12541 ctgacggcag acgagttgcc gccccgcttt gcgccgcgca cccgttaatt gcgttccaca
    12601 cagccctacg ccggtgtatt gcctctttcc cgcaatgcac cggcgttcct cttgcagatc
    12661 atgatcgaca caggggctta tgttaaaatg aactgcctgc agttacttgt aaaagggatg
    12721 ctatgcgaaa tcacctgctc actgcttttc tgattctttt cgggctgctg attggggccg
    12781 gacagtacct tgaacaccgg agcatggcac tgcacgcaga agcggctctg gaacaccgag
    12841 cacaggacct cgcagccgct ttcagtgtgg taattcgctc gcaaggcaga ggagcgcata
    12901 tgtccagaga ccgactggaa gatgccctca tcgatctggt gcatacagcc tctctggaat
    12961 cggtggtgct gcttgatgaa tcgggaaat
//
LOCUS       DAOPWB010000086        12935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4921_length_12935_cov_5.292003, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000086 DAOPWB010000000
VERSION     DAOPWB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12935
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4921_length_12935_cov_5.292003"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..3863
                     /locus_tag="PKN92_08810"
     CDS             447..3863
                     /locus_tag="PKN92_08810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ48933.1"
                     /translation="MLRIYRLPYQSLWFDEWLPHTAMSAGQLSRYLSTFKIVIPEHTS
                     TPAFYVALYLWSKIVGLDPLLLRIFPVLIGCASIALIYFWGSVLFSRRTGITAAFFLA
                     LSPTQIWYSQELRPYGLMLFLALCSVFTLEYALASARKNTRWWVLYYSATTLFIFNHL
                     FSILILIPQGIYIYLRRDFRSFFFWTLPYVLIAALLVFSALNVSHVSVDHTSPAPCPQ
                     LTETLALILGKDVISSHSDMLPVWLANPAAILPTRLQVLLKLRPLMDVLFFLFLIAGL
                     LLLPARYFVLKKKTCQTCKTQSGSKEDKPAPETLPQSGRSEQIFSKIALLLMLVFFPT
                     TILAFIQGVTRLPFTGPGYDAYNVVGIYIAVAFVISTFEKKRLYNIFLLLIALLCGYQ
                     CLIFLPYRTRPDWKAAAAHIRQNCRSGDLILDEWSIGPLSRTHTYLNDLPVQHAQATS
                     FGDVCSQAVSFLKHQEKNEKNQSSVWILTEPLFLSYFIHSRYSPLQTLENALEECGLE
                     VACISFPGGSDISVLKATRSDSQEPHFTSPYIHALYTEDYEQVFSDFEISVQTEQEKE
                     FLIAGLQKSQTFWPIVYTQANIQLGFSFLRNKEYRLAGAFARYLLRSHPYYGLGYYYM
                     GTLLALAGEKERAQAYFTQAFSLYPVVESYSGSFSRELCQPGNTLPGKSASDRLSAFP
                     YQFFVDLERYINETAIPSGDGSGNRMLPESERGKLAAELSEGKVIIEALFSAQDSLAT
                     TEGWLPITKRLPAFWAEALCLFTGDAENTANKYRALIARYPFERIFYDRYDFYLKQYT
                     NGSEYAKKWLDLVSVNPDLAAYAIKKLEETGQFRLQHDQEEDALAAFERALSLGSDSI
                     STAAKAGDLHARSYRWQEALNAYAIVLKKDPYYENVLDKADEILNRLNDESVTQAYWK
                     DIQASNSTQWEVVRRYCAVLENEKRYDEATAIYLSIRDSAEHGCDALLAGARCLRLAS
                     NAQQAFHILNMQDPKRCHPPKLLSYEYILSGQQLTEEQLFTEAIQSFLKATEVGGFDE
                     LAYFGLGSIALQRGDTEGALDYFKKSVDADPENTWHRQCLAVEQQKTGRYQEALENLK
                     ILLEKSPDNEGLRQNVLSLVQEHRDMLREDTVQDLFTSFPDWFSLPPADN"
     gene            3988..4563
                     /locus_tag="PKN92_08815"
     CDS             3988..4563
                     /locus_tag="PKN92_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011868797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNZ48934.1"
                     /translation="MTQEHHPGQVIKSLRESQQLTVEALAERSQLEAEFLTHIEEGYL
                     LPSLAPLMKIARALGVRLGTFLDGESIPGPVIVRQGNAADVLRFTGSGAQKTGTLDFY
                     SLGASKHDRNMEPFLIDVAPSENTAPVLSSHEGEEFLFVLSGAVQVTLGRETHILQQG
                     DSIYYDSIVPHEVRAADTACARILAVVYAPF"
     gene            4603..6267
                     /locus_tag="PKN92_08820"
     CDS             4603..6267
                     /locus_tag="PKN92_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012193123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNZ48935.1"
                     /translation="MIVIDKTIGAVFREQAAQHGTQDFMIYPDRGLRFSWNEFEQRSE
                     ALAKGLLQLGLRRGDHLGVWARNVPDWLTLFFATAQIGVVLITVNTSYKSHELAYVLK
                     QSDMKALAIVDGFRDVSYVDTVYALIPELRQCPRGMLVNSEFPHLKHVLFMGQEKHRG
                     MYSMPELFQMGDQCSDAFLRDVRDSLDCDDVVNIQYTSGTTGFPKGVMLTHRNILNNG
                     FHIGERQQFTDADRVCLPVPLFHCFGIVLGVMATLTHGGTLVMVETFDPLVTLAAVQK
                     EKCTALYGVPTMFNTELNHPMFSMFDLSSLRTGIMAGSPCPIEIMKRVIKDMHCDQIT
                     IAYGLTEASPVITQTSTEDSIERRVSTVGRQLPGMEVKIVDPETGETLGTNQEGELCC
                     RGYNVMKGYYNMPEATATVIDAEGWLHSGDLATVDEHGYYRITGRSKDIIIRGGENIS
                     PREIEEFLHTMPGILDVQVAGVPDERYGEVVAAFIIRKDGSDITEADVRDFSRTRIAR
                     YKVPKYVFFIQEFPLTANGKIQKYKLRELACRELGLDVAGTITAEG"
     gene            6336..6412
                     /locus_tag="PKN92_08825"
     tRNA            6336..6412
                     /locus_tag="PKN92_08825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6370..6372,aa:Val,seq:gac)
     gene            complement(6451..10098)
                     /locus_tag="PKN92_08830"
     CDS             complement(6451..10098)
                     /locus_tag="PKN92_08830"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HNZ48936.1"
                     /translation="MRYCPCILIALCMAWNAAAFNEPVDQAGPLKAVLNVPDAMTEKD
                     SSLSGSLLLHCDYASAEGNFTLRTIDGWQIEPSEKTDFFLNKGESREFPLSVTADPDS
                     FNAIYPVHAYIDFKTEEGQFTLHPVQLIYTRFEAVPRPDSILPWQPFALKHSRTKLLA
                     IPVHRMRVQTVGRAVTILPTGFSGTERATHAEILPYGVLSLPEPHETLFAHPPWKGGA
                     RGSVIREYYLNLPANSPIRFQSAVAIGKHNEGQEPPSDGVTFRVRLVPENAPEDAPGE
                     ILCETHTSSFVWQDLSCDLSAYAGQTVRLLLETHPGQNLDTTCDRGLWGDPEILINKT
                     EEDELPYSSQKYLLGAITIEGHTSEVFFQPGRRGLLDGTMTFSGTGGEVSFQGFSVKV
                     FGEELKENLGASTLAAVQIEDKNNGIRIRHSFQNPLGDFELIGDLSVPEGTALRVELR
                     IGAAPSPKPWLICLLEQVETGPWNARAARVYAGMGNVICNPEAFTLAPDGHQLATSFV
                     GFDFESGLSLVQALKAPPSRLKVSPKDRVYTLEAGVVADYHFLPAENVWAAVRAWRDI
                     NGLNAGRGVEQLAGRFTFDLWGGRYAPSQEALQRSFRYGLTNSVVIWHSWQRYGYDYR
                     LPDITPANPNLGSLEEFQSLIRTCQEQGALFAPHDNYIDFYPDAEGFTYDAVAFQKNR
                     EPVWAWLNEGRGAQSFRWRPTAFRPFMEKNIRWVRDWLHPDAYFIDVWASLMPCDTWT
                     QDGQLEDRMCCRKEWQSAFYWIQDALGGKAPMISESGHDQLVGYVDGAQANHLRVDAD
                     PPPGRDWGSTWRIRCSDAERIPWSDMAYHDRFVYQGAGYNPRYSAGWRQDLHGIFSDD
                     YISTEMLTGHPAMTPAAFSRDGVRKYWLSNDFITRIALNPMEYHHFEDDDIHRQHIRY
                     ANGAQIWVNRGEKDWDIKGHVLPPFGFYAEAGDAACAIEKKQETLVEWSRAPERLYLN
                     ARRAPEACLPLQLLAADITSVGGRTIQLSLNWDLRAPLDPAMRIFVHFIDSKDNIAFQ
                     ADHPFPAEAASEAKAFSTAAQAQLPPECQTGDHFEIRVGVFQPGKGQCLMEQAHHERR
                     FIAAGTLVFEGEEGKLPRISYAKQVLETDHYYLRMNREGKAVSFEEAVTDGAFRLTRE
                     GARLILLPLPDSPAFDISLNLEALPFAAAKPGQITTEKQDGTRLHESLEESGTELSLH
                     VAADTFALYLE"
     gene            10244..12901
                     /gene="mutS"
                     /locus_tag="PKN92_08835"
     CDS             10244..12901
                     /gene="mutS"
                     /locus_tag="PKN92_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNZ48937.1"
                     /translation="MTEKWMQLSEISPHHTTPAMRQYLQAKMESADTLLFFRMGDFYE
                     LFFEDALEAASLLNLALTSRDGAEKERRIPMCGVPVRAVDSYLAKLVRLGRSVSICEQ
                     MENPKDVKGIVKRQVVRTVTPGTVLEPELLEDGQNNFLAALCANGEEAAAAFADMTTG
                     EFLTVCVSEASGTLFDEIARLTPAEILLPDENQEGLWSEVKKRFPDTALARRPSDEFD
                     PAYGKETLLQYYRLKTLQGLGELALSGPALGCAGAVISYLRETQREGIPQLGLPRHQR
                     PSAYVALDTNTQRNLELTATMHDKSKRGSLLGVLDKTLTGMGERKLRHWILHPLAELE
                     PLKERLDGVNEFFDEITLRLSLREALKGMPDLERLLGRITASAGNARDVKSMGTTLSL
                     LPEILRIAESFQSPLLQELQSALDPLEDIAYLIETAIADEPPLTLNEGGLIREGYHEE
                     LDRLRDLVRGGVNWIAKLQQAERDRTGIAKLKVGYNRVFGYYIEVSRGQAHQVPDDYQ
                     RKQTLANAERYITPALKKHEEEILTAQEKMQDLEYEIFITVRNQVAAASERIRKTADA
                     LASLDLISSLAEVAATHNYCRPELDLSGEIHIRDGRHPVVEDLNKQGEFVPNDTRLGE
                     NGSRMLIMTGPNMAGKSTYLRQVALICLMAQMGSYVPASQARIGLVDRIFTRVGASDN
                     LVRGESTFMVEMIETAAILNSAGENSLLVLDEIGRGTSTFDGISIACSIAEFLHNHIH
                     KPLTLFATHYHELNELENKMARIKNFHVTHKEVGNKIIFLRKLALGGSTHSFGLHVAR
                     MAGMPPSLVDRANEILAMLEEKNLNNKIGINLKKIPTQNFQLNIFDGLAEDLKRVKEI
                     LENVDINSLTPVEALMKLNELKSVVRSNS"
BASE COUNT         3170 a         3329 c         3176 g         3260 t
ORIGIN      
        1 aatggccact tggaaactct cattatacat ggaccagttt acagggggta caccacaact
       61 acacggcaag tgtcgtgaat ctcttttttt cccagataag cagcagaggc cccatgtcca
      121 taagaaacac acaaataaaa aaaatgcatt tttctgtcac ctgtcctctc tatgagatag
      181 atttttaact gacgggcaaa ttctgaggaa atttgttatc ataactgcag ttttcaagcc
      241 ctaccccgga ctttctgcgc cgtcagtcgg gagggatgcc cttcgctttg catcttttac
      301 agagcgactt tctattatga gcaggtgcga gactcgtaat gtctgatttt ttttatgcag
      361 tttgtaacaa gtgttcccgg agcggaacct tcatcaaaaa atacataggg gcgcatccgc
      421 tggtttttat aataggcatt ggtttcttgc tccgtattta ccggctgcct tatcaatcgc
      481 tgtggtttga tgaatggctc cctcatacag ccatgtccgc aggccaatta tcacgctact
      541 taagtacttt taaaattgta attcccgaac acaccagtac accagccttt tatgtggcac
      601 tgtatttgtg gtcgaagatc gttggcttgg atcctctgct gttgcgaatc ttcccagttt
      661 tgatcggatg cgcctccatt gcactgattt atttttgggg atctgttcta ttttcacgtc
      721 gcaccggtat tacggctgct ttttttcttg ctttatcccc tacacagatt tggtactcac
      781 aggagctcag accctacggg ctcatgcttt ttcttgcgct ctgttctgtc ttcacgcttg
      841 agtatgcatt agcatctgcc cgaaaaaata cccgctggtg ggtgctttat tacagcgcaa
      901 ctactttatt tatctttaat cacctctttt ctattcttat tctgattcca caggggattt
      961 atatttatct gcgccgggac ttcagatcat tttttttctg gacactcccc tatgtgttga
     1021 ttgctgccct tctcgttttt tctgctttga atgtttcgca tgtttctgtt gatcacacaa
     1081 gcccggctcc ctgccctcaa ttaactgaaa cgctggcatt aatacttgga aaggatgtca
     1141 taagcagcca ctctgacatg ctgccggtct ggttggctaa tcctgctgcc attcttccaa
     1201 cgcgacttca ggttttactg aaattacgcc cactcatgga tgtgttgttt tttctgtttc
     1261 ttatcgccgg ccttctgttg cttcccgccc gctatttcgt tttaaagaaa aagacgtgcc
     1321 agacatgtaa gacacaatcc ggctcaaagg aggataagcc tgccccagaa accctgccgc
     1381 aatcaggcag gtccgaacag atcttttcaa aaatcgctct gcttctcatg ctcgtttttt
     1441 tcccgaccac tatattggct ttcattcagg gcgttacaag acttcccttc acggggccgg
     1501 gttatgatgc atataacgtg gtcgggatct acattgctgt cgccttcgtg atcagcactt
     1561 ttgaaaaaaa acgcctgtac aatattttcc tgcttctgat cgctctttta tgcggttatc
     1621 aatgccttat ctttctccct taccgaaccc gtcctgactg gaaagcagcc gcagcacata
     1681 ttcgtcaaaa ttgcaggtct ggagatctga ttctggatga atggagcatt ggtcccctgt
     1741 cccgcacaca tacttattta aacgatcttc cggttcaaca cgcgcaagcc acctccttcg
     1801 gcgatgtctg ttcacaagcg gtttcttttc tgaaacatca agaaaaaaat gaaaagaatc
     1861 aatcttctgt ctggatactg acggaaccgc tttttttgtc ttatttcatt cactcccgtt
     1921 attctccttt acagacactt gaaaatgccc tggaagaatg cggattggaa gttgcatgta
     1981 tttctttccc tggcgggtcc gatatcagtg ttctgaaagc cacaagaagc gactcgcaag
     2041 agccgcattt taccagtcct tatattcatg ccctttatac agaagattac gaacaggttt
     2101 tctctgactt tgaaatttct gtgcaaacgg agcaagaaaa agaatttctc attgcaggcc
     2161 tgcaaaagtc tcagactttt tggccgattg tttatacaca ggcaaatatt cagcttggct
     2221 tttccttttt gcgcaataaa gaataccgtc tggcaggtgc ctttgcccgg tacctccttc
     2281 gttcccatcc ttattacggt ctgggctact actatatggg caccctgctg gcgctggctg
     2341 gagaaaaaga gcgggctcag gcttatttta cacaggcttt ttctttgtat cccgtagtgg
     2401 agtcttattc aggaagcttt tccagagaac tatgtcagcc cggaaataca cttcccggaa
     2461 aaagtgcatc agatcgcctg tccgctttcc cttaccaatt ttttgttgat ctggagcggt
     2521 atatcaacga aacggctatt ccttctgggg atggctcggg aaacagaatg cttccggaat
     2581 cggaaagagg aaaactggct gcggaactat ctgaaggcaa ggtgattata gaagcactct
     2641 tctctgcgca ggacagtttg gctacaacgg aggggtggct gcctattacc aaaagacttc
     2701 cagccttttg ggcagaagcg ctctgtctct tcacaggtga tgctgaaaac acggcaaata
     2761 aatacagagc cttaattgcc cgctatcctt ttgagcgtat attttatgat cgctatgatt
     2821 tttatctgaa gcagtatacg aacggaagtg agtatgcgaa aaagtggctt gatctcgtgt
     2881 ctgtaaatcc tgatttggct gcctatgcca tcaaaaaact tgaggagacc ggacagttcc
     2941 ggcttcagca cgatcaggaa gaagatgcgc tggctgcctt tgaaagggct ttatcgctgg
     3001 gttcagattc tatcagtaca gctgcaaaag caggagatct ccatgcacgc agctaccgtt
     3061 ggcaagaagc actgaacgct tatgccattg tgcttaaaaa agacccttac tatgaaaatg
     3121 tactggataa ggcagatgag attctgaacc gtttaaatga tgaaagcgtc acacaggcgt
     3181 attggaagga tattcaggcg tccaacagca cgcaatggga agtggtacgc cgctattgcg
     3241 ccgtcctgga aaacgaaaaa cgctacgacg aagccactgc tatctatcta agcatcaggg
     3301 acagcgctga acatggctgc gatgcccttt tggcaggtgc ccgctgcctg cgccttgcca
     3361 gcaacgcaca acaagcattt cacatattga acatgcagga tccgaagcgt tgtcatcccc
     3421 ctaaactcct cagctatgag tatattcttt caggtcaaca attgacggaa gagcagttgt
     3481 ttactgaagc catacaatcc tttctcaagg ccacagaagt gggcggcttt gacgaattgg
     3541 cgtattttgg tctgggcagt attgctctgc agcgtggaga tacggaaggc gccttggatt
     3601 atttcaagaa atcagtagat gccgatccgg agaatacctg gcatcgtcag tgcctggctg
     3661 ttgaacagca aaaaacaggc cgatatcagg aagcgctgga aaacttaaag atccttctgg
     3721 agaaatcacc cgacaacgag ggattgcggc aaaatgtttt atctctggta caggaacatc
     3781 gcgatatgct tcgggaagat acagtgcagg atctttttac ttccttcccg gactggtttt
     3841 ccctgccgcc agctgacaac tgattgtctg tgaacgtctt cgggtagcag caacgggctg
     3901 tgattggtta tactaatcgc cgaggtacgc aggcattcgg ggcggcggct tatttatgaa
     3961 aacaacccat cagccatgga gtgcagtatg actcaggaac atcatcccgg acaggttatt
     4021 aaatctttgc gggaaagcca gcaattgaca gttgaggccc ttgccgagcg ttctcagctg
     4081 gaagctgaat ttcttacaca tattgaagag ggatacctgc tcccctctct tgcaccgctc
     4141 atgaaaattg ccagggctct cggcgtgcgt ctcggcactt ttcttgacgg ggaatccatc
     4201 cccggtcccg tgattgttcg tcagggaaac gccgccgatg ttctccgctt cacaggatcc
     4261 ggcgcgcaga aaacaggcac gctcgatttt tattctttag gcgcttccaa acacgaccgg
     4321 aacatggaac ctttcctcat tgatgtggcc ccctcggaga acacagcccc cgttctttct
     4381 tcccatgaag gtgaagagtt tctgttcgtt ctgagcggag cggtgcaggt gaccctggga
     4441 cgggaaacgc acatactgca acagggcgac agcatctact atgattccat tgtgcctcat
     4501 gaagtccggg cggcagacac tgcatgcgcc cggattctcg ctgttgtcta tgcgcccttc
     4561 tgattgaaaa caactgaata cgaattccct ctggagcgtt gtatgatcgt catagataaa
     4621 acgataggtg ctgtgtttcg tgaacaggca gcccagcacg gcactcagga ctttatgatt
     4681 tatcccgaca ggggtctgcg cttctcctgg aatgaatttg aacagcgaag cgaagcgctt
     4741 gccaaaggac tgctgcagct tgggctccgc cgtggagatc atctgggcgt ttgggcgcgc
     4801 aatgtacccg actggctcac gcttttcttt gccactgccc agataggtgt tgtgctgatt
     4861 acagtcaaca cgtcctacaa aagccatgaa cttgcctatg tgctgaagca gtcggatatg
     4921 aaagcattgg cgattgtgga cgggttccgt gatgtcagct atgtagatac cgtttacgct
     4981 cttattccag aactgcgcca gtgtccccgt ggcatgcttg tcaatagcga gttcccgcat
     5041 ttgaaacatg tccttttcat gggtcaggaa aaacatcgcg gtatgtacag catgcctgag
     5101 ctgttccaga tgggtgatca atgttccgac gcatttctac gggacgtccg tgactcactg
     5161 gactgtgacg atgtagtcaa cattcagtac acttcgggca ccaccggatt ccctaaaggg
     5221 gtcatgctga cacaccggaa tattctcaac aacggctttc atatagggga gcgccagcaa
     5281 ttcaccgacg ccgaccgggt ctgtctgcct gtgcccttat ttcactgctt cggtatcgtg
     5341 ctgggcgtca tggcaaccct tacccacgga ggcacgctgg tcatggtgga aacctttgat
     5401 cctctcgtca ctctggcagc ggtacagaag gaaaaatgca cggctcttta cggcgtgccg
     5461 accatgttca atacggaact gaatcatccc atgttttcca tgtttgacct cagttcgctg
     5521 cgcaccggaa tcatggcggg ctctccctgc ccgatcgaaa tcatgaaacg tgtcattaaa
     5581 gatatgcact gcgatcagat cacgattgcc tacggtctga ctgaagcatc accggtaatt
     5641 acccagacaa gcacagagga ttccatagag cgccgtgtca gtactgtagg cagacagctg
     5701 ccgggtatgg aagtgaaaat cgttgatcct gaaacaggcg aaacactcgg gaccaatcag
     5761 gaaggcgaac tctgctgccg gggctacaat gtgatgaaag gctattacaa tatgccggaa
     5821 gccactgcaa cagtcataga cgcagaaggc tggctgcaca gcggggacct cgccacggtc
     5881 gatgaacacg ggtattaccg gattaccggg agaagcaaag acatcatcat ccggggcggc
     5941 gagaacattt ctccccggga aattgaagag tttctgcata ccatgcccgg cattctggat
     6001 gttcaggttg caggtgtccc cgatgagcga tatggagagg tggttgccgc tttcatcatc
     6061 cggaaagacg gctctgacat cacagaagcc gacgtgcgag acttttcacg cacccgaatt
     6121 gcccggtata aagtgcccaa atatgtcttc tttattcagg aatttccgct gactgccaac
     6181 ggaaaaatcc agaaatacaa gctgcgcgaa ctggcgtgcc gtgaactggg tctcgatgtg
     6241 gcaggcacca ttacggcgga agggtagcct tctcatcctg ccggttcctt acttttgatt
     6301 cagcaccaaa atgtttgcta tactctccgt gctgtgggcg attagctcag ttggttagag
     6361 tgccacgttg acatcgtgga ggtcgcaggt tcgattcccg catcgcccac catcccctcc
     6421 tatttttcct ttccatttct ctgttccgct ttattccaga taaagtgcaa acgtatccgc
     6481 tgccacatgc aggctgagtt ctgtgccgct ttcctcaagg gattcatgca gccgggttcc
     6541 gtcctgtttc tcagttgtta tttggccggg ctttgccgca gcaaagggca atgcctccag
     6601 attcagagaa atgtcaaaag cagggctgtc aggaagaggc agaagaataa ggcgcgcccc
     6661 ctcccgggtc agacggaaag cgccgtcggt cacagcctcc tcaaaggaaa ccgcctttcc
     6721 ctctctgttc atgcgaaggt aataatggtc tgtttcgagc acctgttttg cataggaaat
     6781 ccggggcagt ttcccttctt ctccttcaaa aacaagcgtt cctgcagcaa taaagcggcg
     6841 ttcatgatgc gcctgctcca tcaggcactg ccctttcccg ggctgaaaca cacctaccct
     6901 tatttcaaag tgatcgcctg tctggcattc aggaggcagc tgtgcctgtg cagcagtgga
     6961 aaaagctttc gcctccgatg cggcctctgc agggaaagga tgatccgcct gaaatgctat
     7021 attgtcttta ctgtcgataa aatgaacaaa aatccgcatc gccggatcca aaggcgcccg
     7081 gagatcccag tttaaagata attggattgt gcggccgccg acagacgtta tatccgctgc
     7141 aagcagctgc agaggcaggc aggcttcagg agctctccgg gcatttaaat acagcctttc
     7201 cggagcacgg ctccactcaa ccagcgtctc ctgcttcttt tctatggcgc aggcagcgtc
     7261 gcccgcttca gcataaaaac cgaaaggcgg cagaacatgt ccttttatgt cccaatcttt
     7321 ctcgccccgg ttaacccaga tctgtgcacc attggcgtac cggatatgct gccggtgaat
     7381 gtcatcgtct tcaaaatgat ggtattccat ggggttcagg gcaatacgtg ttatgaaatc
     7441 attggaaagc cagtattttc ggactccgtc ccggctgaat gctgccggtg tcatggcagg
     7501 atgacctgtc agcatttcgg ttgaaatata atcatcgctg aaaatgccat gcagatcctg
     7561 acgccacccg gcagaatacc gggggttata tcctgccccc tgataaacga agcggtcatg
     7621 atacgccata tcgctccagg gaatccgctc agcatcgctg caccgtatgc gccaggtgct
     7681 tccccagtcc cgtcccggcg gcgggtccgc atccacccgt aaatggttcg cctgcgcacc
     7741 gtcgacatac cccaccagct ggtcatggcc gctctcggaa atcatgggcg ccttgccgcc
     7801 caatgcatcc tgaatccaat agaaggcgct ttgccattcc ttccggcagc acatacggtc
     7861 ttccagctgc ccgtcctgcg tccacgtatc acagggcatc agacttgccc atacatcaat
     7921 aaaataagca tccggatgca gccaatcccg aacccagcgg atattctttt ccataaaagg
     7981 gcggaatgct gtggggcgcc agcgaaagga ctgcgctccc ctgccttcat tcagccacgc
     8041 ccagaccggc tccctgtttt tctggaaagc gacagcatcg taggtaaaac cttcagcatc
     8101 ggggtaaaaa tcaatgtagt tatcatgagg agcaaagagc gctccctgct cctgacaggt
     8161 gcgaatcagg ctttgaaatt cttccagaga acccagattg ggatttgccg gcgtgatatc
     8221 gggcagccga tagtcatacc cgtaccgctg ccagctgtgc cagattacga cgctgtttgt
     8281 cagcccgtag cgaaagcttc tttgaagcgc ttcctgtgat ggcgcatatc gcccgcccca
     8341 gagatcaaag gtgaaacgcc ctgccagctg ttctacgccg cgtccggcat tcaaaccgtt
     8401 tatatcacgc catgcacgca ccgctgccca gacattttct gccggaagaa aatgataatc
     8461 cgcaacgact cctgcctcca gggtataaac acggtctttc ggcgatactt tcaggcggga
     8521 cggcggcgct ttcagtgcct gcacaagaga aaggccggac tcaaaatcaa acccgacaaa
     8581 agaagttgca agctgatgcc cgtcgggcgc aagtgtaaaa gcttcaggat tacagatgac
     8641 attacccata cccgcataca cccgcgctgc ccgggcattc cacggccctg tttccacttg
     8701 ttcgagcaag caaatcagcc agggcttcgg actcggcgcc gccccgatgc gcaattcaac
     8761 ccgaagcgct gtcccttccg gaacagacag atctccgatc agttcaaagt cgcccagagg
     8821 attctgaaaa gaatgacgga ttcggatacc gttgtttttg tcttcaatct gaacggcggc
     8881 aagcgtactg gcacccagat tttctttcag ttcttcacca aacactttca ctgaaaatcc
     8941 ctggaaagac acttctcctc cggtgccgga aaaagtcatt gtgccatcca gaagccctct
     9001 gcggccgggc tgaaaaaaca cctccgatgt gtgcccctct atggtaattg cgccgagtaa
     9061 atatttttgt gatgaatacg gaagttcgtc ttcttccgtt ttatttatca ggatttcggg
     9121 atccccccac agccctctgt cgcaggttgt atccagattc tgtcccgggt gcgtttcaag
     9181 cagaaggcgt acggtctgcc cggcataggc gcttaagtca caggacaaat cctgccagac
     9241 aaaagaactg gtatgggttt cacataagat ttcacccggg gcatcttcgg gcgcattctc
     9301 cggcacgagg cgcactctga aagtgacgcc gtcactgggc ggctcctgcc cctcattatg
     9361 ctttcctata gccacagcgg actgaaaacg aatgggagaa ttggcaggaa gattcaagta
     9421 gtattcacgg ataacagaac cccgggcgcc gcctttccac ggaggatggg caaaaagtgt
     9481 ttcgtgcggc tcaggaagcg acaaaacccc ataaggaaga atttcggcat gcgttgctct
     9541 ttccgtcccg gaaaatccgg tcggcagaat ggtgaccgct ctcccgactg tctgcacgcg
     9601 cattcggtga acagggatcg ccagaagttt tgtcctgctg tgtttaagcg caaagggctg
     9661 ccagggcaat atgctgtccg gcctgggaac cgcctcaaat cgtgtataga tcagctgaac
     9721 agggtgcagc gtaaattgcc cttcttctgt tttaaagtct atataagcgt gaactggata
     9781 gatcgcattg aaactgtcag ggtctgccgt aaccgacagg ggaaattcgc ggctctcccc
     9841 tttgttcaaa aagaaatctg ttttctcact gggttctatc tgccacccgt caatagtgcg
     9901 aagcgtaaaa ttgccttctg ctgaagcata atcgcaatgc agcagtaaag agccagatag
     9961 ggagctgtcc ttttcggtca tcgcgtccgg gacgtttaaa acggccttca gtggccctgc
    10021 ctgatcgaca ggttcattga aggcagcagc attccaggcc atacagaggg cgataagaat
    10081 acaggggcag taacgcatgt tgtttccttt gcggtttact gaaattgtat caggaggaga
    10141 ggctttccgg ctaaatgttt ttcatgcgtg cacgttcacc gctggattgc ccgcccggcc
    10201 tctgaggtga tgtagttcag tctcaacagg tataataccc gtcatgactg aaaaatggat
    10261 gcaactgagc gaaatctccc cacaccacac cacacccgcc atgcgccaat atctacaggc
    10321 aaaaatggag agtgcggata cgcttctttt ttttcgtatg ggcgattttt atgagctctt
    10381 tttcgaagat gctctcgaag ccgcttccct tctgaatctg gcgctgacct ccagagacgg
    10441 ggcggaaaag gagcgtcgaa ttcccatgtg cggtgtaccg gtgcgtgctg tggacagcta
    10501 tctggcaaaa cttgtccgcc tggggcgctc cgtatccatc tgcgagcaga tggaaaatcc
    10561 caaagatgtg aaaggtatcg tcaaacgtca ggtagttcga accgttacac cgggcacggt
    10621 acttgaaccg gaactgctgg aggacgggca aaacaatttc cttgccgctc tttgcgctaa
    10681 cggtgaagag gccgccgccg cttttgccga catgacaaca ggagagtttc tgactgtgtg
    10741 cgtttctgaa gcatcgggga cgctctttga tgaaattgcg cgtctgactc ctgcggaaat
    10801 tcttttacct gatgagaatc aggaggggct gtggtcggaa gtaaaaaaaa ggtttccgga
    10861 caccgccctt gcccggcgtc cttccgatga atttgacccg gcctacggaa aagaaacgct
    10921 gctgcagtat tatcgattga aaacgcttca aggactgggt gaactggctt tatccggccc
    10981 ggcactcgga tgcgcaggcg ctgtaatttc ctatctcaga gaaacacagc gggaaggtat
    11041 cccccagctg ggtcttccga ggcatcagcg cccctctgca tatgtcgctc tggatacgaa
    11101 tacgcagcgc aatcttgagt tgacagcgac catgcacgac aaatcgaaac ggggctcgct
    11161 tctgggtgtg ctggataaaa ccctgaccgg catgggcgaa cggaaactcc gccattggat
    11221 tctgcaccct ctggcggaac tcgaaccgct gaaagagcgg ctggatgggg tcaacgagtt
    11281 ttttgatgaa ataacacttc gtctttcttt gcgggaagcc ttgaaaggaa tgcccgatct
    11341 ggagcggctt ctgggcagga tcaccgcaag cgccggcaat gcaagagacg taaagtcaat
    11401 gggcaccaca ctctctttgc ttccggaaat cctcagaatt gcggaaagtt ttcagtctcc
    11461 tctccttcag gaattgcagt ccgctctgga tccgctggaa gatatcgcat accttataga
    11521 aactgccatc gctgacgagc cgccactcac attgaacgag ggcggtctga tccgtgaagg
    11581 ataccatgag gaacttgaca ggctgcggga tctggtgcgt ggcggcgtaa actggatcgc
    11641 aaaactgcag caggcagaac gggatcgtac ggggatagca aaactgaaag tcgggtacaa
    11701 ccgcgtattc ggttattaca tagaagtgag ccgggggcag gcgcatcagg tccctgatga
    11761 ttaccagcgc aaacagacgc ttgccaatgc ggaacgctat attacgcccg ccctgaaaaa
    11821 acatgaagaa gagatcctta cagcgcagga aaaaatgcag gatctggaat atgagatttt
    11881 tatcacggtg cgcaatcagg tcgctgctgc gtctgaacgc atccgcaaaa ctgccgatgc
    11941 tcttgcatct ctggatctga tctcttcact ggcggaagtg gcggcaactc ataactactg
    12001 ccgtcctgaa ttggatctgt ccggagaaat ccatatacgg gacggacggc atcctgttgt
    12061 ggaagatctc aataagcagg gcgaatttgt acccaacgat acacggctgg gcgagaacgg
    12121 ctcccggatg ctgatcatga cggggccgaa catggcggga aaatccacct acctccgtca
    12181 ggtggcatta atttgcctca tggcacagat gggcagctat gtgccagcct cacaggcgcg
    12241 catcggtctg gttgaccgca ttttcacccg ggtcggcgca tcggacaatc tcgttcgggg
    12301 cgagagtacc tttatggtcg aaatgatcga aacagccgca attttaaaca gtgcgggcga
    12361 aaacagcctc cttgttctcg atgaaatagg cagaggcacc agtacatttg atggcatcag
    12421 tattgcatgt agtattgccg aatttttaca caatcatata cacaaacccc ttacactttt
    12481 tgctacccat tatcatgaac tcaatgagtt agaaaacaaa atggcacgta tcaaaaactt
    12541 tcatgtgaca cataaagaag ttggtaataa aattattttt ttacgtaaac tggctttggg
    12601 tggcagcaca catagttttg gtttgcatgt agcacgtatg gcaggtatgc ccccatcatt
    12661 ggttgacaga gccaatgaaa tattagccat gttggaagaa aaaaacttaa ataacaaaat
    12721 aggcattaac ctcaaaaaaa tacctacaca gaattttcaa ctcaatattt ttgatggatt
    12781 agcagaagat cttaaaagag taaaggaaat attagaaaac gtagatatta attcactgac
    12841 ccctgtagaa gccttgatga aactgaatga gctgaagagt gtggtgcgaa gcaatagcta
    12901 aactcatagc taaggctatt catgtaattc actta
//
LOCUS       DAOPWB010000087        12828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_4976_length_12828_cov_4.642527, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000087 DAOPWB010000000
VERSION     DAOPWB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4976_length_12828_cov_4.642527"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1013)
                     /locus_tag="PKN92_08840"
     CDS             complement(108..1013)
                     /locus_tag="PKN92_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNZ48938.1"
                     /translation="MAAILQFQQRPGLLSAYKNIFMPGRAAYQGIEALPKLEAQWNKA
                     VDPAARIQQYRDVCGFENDGTFPILYPHVLTFAMHIHLLADKRFPAKAFGAVHARSHM
                     LQRRAISETEPVDLKCFFSDARILKAGLEVDVTTLVSSGGSVIWESVNSYLFRGKKFG
                     EIGDAHPWSAFSELGEEAFTASWHVPKKMGRTYARITGDFNPIHISRIMAKLFGFKRD
                     IIHGMWSLARCLTALPGPAIEGPQRLDVAFKGPVFMDSSCTLKGTDQDGARRFDLFCG
                     KNPRPTIVAQLAPCSETDTLLTQKS"
     gene            complement(1162..2046)
                     /locus_tag="PKN92_08845"
     CDS             complement(1162..2046)
                     /locus_tag="PKN92_08845"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNZ48939.1"
                     /translation="MTALVRTLKRLRFVLTAAVLMFLPLIESGCTQQAVIRGAIYDAA
                     GETLPGVSVHLEGQDKEALSNANGSYSIRTVRGAASLHFYKTGYTSARVAVDSLAFGS
                     NEMPDVVLMPLPAEEGVFYRQALRYEALEHPRINRYTIGKGEPIFGTPVQPTLILPWE
                     DPEKAPEEEKLRFLAYKVQPYNALLHKLRFLTLKEEKKKEQEQKPAKEAEAGEKILIA
                     DTALPLIARILDDSDGQLVELYPGYPLEPGIYAIHWGALSGYDSLDPRAFLFAIGEKE
                     EAEGEQSKEGELKESVPN"
     gene            complement(2043..2822)
                     /locus_tag="PKN92_08850"
     CDS             complement(2043..2822)
                     /locus_tag="PKN92_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNZ48940.1"
                     /translation="MSLYPYGLQLYSIRDHCQKDLMKALKPLRKAGYKWVELAGLYGK
                     SAAEFKTFLDQAALKAVSMHVGIEELSAQPEMVMLQAKTLRAPYIVVPWLGEEQCPDR
                     ASWIDAARLLDELGAAFRKEGYLLCYHNHAHEFTRYGTDRILDLILKTADPQHLQLEL
                     DLGWAGVAGISFEDLLDKYQGRIPLVHVKDYQPDHDGGPATFTELGKGIIPWRHFLPD
                     LKNAGVRFYIVEQDDSQGDSLEAALRNGHYMNRITGQERYL"
     gene            2930..3847
                     /locus_tag="PKN92_08855"
     CDS             2930..3847
                     /locus_tag="PKN92_08855"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HNZ48941.1"
                     /translation="MTVKYFSGLMAALMLLPVCAGAASLRAVLQKEQIDVLAQEQLVT
                     SYKVNKKQKYPYFFPVNGPVSHASLTTETSEPFPHHRSLFFGCDRVNGGNYWQDSLRA
                     GQIVSLELKVEKAEGEEVVFTDTCEWRQPGKAPVLRDYRQITVSAPSDSVHVIDFQIR
                     LVPLTALHIEKTNHSLFAARMVPELSAEKGGVLMNAAGDTNEAGTFGIVSPWCDYSGS
                     RQGNTEGLAIFQHPENPCYPAPWFTRDYGFFSPTPLYWPPSDDGIRLQEGEELLLRYR
                     VILHAGTAEEAGIPALFEAYTAGAASCGN"
     gene            3869..4264
                     /locus_tag="PKN92_08860"
     CDS             3869..4264
                     /locus_tag="PKN92_08860"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HNZ48942.1"
                     /translation="MVDKNRKKQFFDQLKRDQRERDAGYRAQALKLFPHMCQRCGREF
                     SGRNLRELTVHHKDGNHFNNPPDGSNWAMLCLYCHDDEHGTLEQRGIADAGDFALKKD
                     DSLTHNPFAQLKKRFAESDGEQNGPGEEP"
     gene            4261..7683
                     /locus_tag="PKN92_08865"
     CDS             4261..7683
                     /locus_tag="PKN92_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48943.1"
                     /translation="MKIRILFAVTLLCLLTVHTGTAAAAEGGGTALFFEDPAFPADER
                     TVRCLKELLTLEGYALMPVNAEALCSTAELTAAHNTLLVLADAARLPMAAIPPVTAYL
                     NQGGNLLALNAPLWQERLTRFGDEWLSLEAYRRRMAGTAPSHCFLDFRAESLASWHRS
                     AYRMETDTRFSLVVADELGEGKKALLVDMPDMEGWDSACRSFETSPYPPGHVMTVFTA
                     KGGPRTTRLAVELRERDGSRWMATVPLSPQWEQYVLLPEEFEFWESVYARKDTRLNPA
                     NVEMIAFTLAYSHTGHLHGQHSFAVADVGSAPLDEKYRAARKEIPVPAFEILAPSYKF
                     YPMSDVTALVTAEDGLLPAGAPAPMPRAMMSMHPRPEAGGFDKGRHWRWLPLLEARGS
                     DGTVRGYPAAMFFQDSTLCAGACRASFAIDDPGWYVSSAGKELLRSVIQRMRMPLFLL
                     DGGADCYTAFEDQTVQVGATVANRSGGDEAVQLEFAIKDKNGFHTEKRNMQTVIPAGK
                     EMAVESSLMTLEGRDMPVEISVRLLQKGEVLDEAHHMLHLWRPSASPSFVAAGKGRFD
                     LNGKRWIPHGVNYMPSSGIGVEDRTYFEKWLGADAYHPRVIQRDLERCVAMGCNALSI
                     FIYHESLKGHNLLDLLRRMDDLGMKANLSLRPGTPMDFKWDLIKELIEVHRLSENDTV
                     FAYDLAWEPQFRPHEREPFNKDWEAWVVERYGSIENAEKDWGEPFLRDENGAPLNITP
                     EMVGRAGQGAPRMAAYRRFLDTLLFEKYNQARTLVHNIDPNHPVSFRMSMAGDPTDWQ
                     TDALLYDFAYLGGAVDIFEPEAYGRIGDWEKVKPGAFTSAYARWANPALPVLWAEAGV
                     HVWDMSSMKQGEDMLQFQAQYFDDFYRMLLMSRVDGIFWWWYPGGYRFDEKSDYGIIN
                     PDGTDRPSTKVIRKYAPLISQMETLPPVDEWLLFDRDKHNNGLTGIYSELKKDFWANI
                     EAGKVPGLRTSGTGTDTRTCPALAVGNTPLNGNNPPKYMDSVFDQVARKDEQGGWIPL
                     KNRASVFLPQTEELNLQISLTNLGEAALVPPDENRNQAGDVYLTVSGALSFRVPITQD
                     VGRFQSLTMELSLTDAIRDQLKASGTIRLQMEARDRSAFGSVFELQLKKMQP"
     gene            7696..9099
                     /locus_tag="PKN92_08870"
     CDS             7696..9099
                     /locus_tag="PKN92_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HNZ48944.1"
                     /translation="MKKVSRREMLCAMAAGTTVALLPSYLAAEQKAKRPNIVFILADD
                     LGYGGLGCYGQKKIKTPCIDRMAAEGIRFTRAYAGSTVCAPSRCALMTGLHTGHCRIR
                     GNHSLVRRDALALLPDDVTVAETLQKAGYRTALIGKWGLGEIGSSGAPRNKGFDEFFG
                     YTDQGEAHNYYPEWLWRNEEKEPLPGNVLEMPRVSKERTTYSHDLFTKEALSFLGRQN
                     GEKPFFLYLAYTLPHANNEATRVREHGMEVPDEGSYAGEDWPPAQRSHAAMISRLDRD
                     VGRVLDLLRERGLAENTLVFFASDNGPHKEGGADPAFFEDSGPFRGFKRDMYEGGIRV
                     PAIAWWPGVISPGQVSDHCWAFWDFPVTASSLAGVEAPSGDGISLLPLLTGKGHQETH
                     EVFYWEFHEGGCRQAVRFGEWKAVKPGKGEKLELYRVEEDPGEENNCAGLYPEKVKEA
                     LTYLRRERSESEDFPRR"
     gene            complement(9100..9792)
                     /locus_tag="PKN92_08875"
     CDS             complement(9100..9792)
                     /locus_tag="PKN92_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNZ48945.1"
                     /translation="MRTTTRLTLPSLAEEKFFLGLDPSISARLDTFAWHRRYDPRQII
                     YFPDDRCDYVYWVRSGRVKITHVTERHRELTYRHLFKGDLFGEEGLADRSQRKDYAEA
                     LTDTVLTLVRYDDFFQVVQEEGTLSLALARHCCRRALDVEKSLSDLLFMEVRQRIACR
                     LLELSGQEAEREDNGPLRLTHQELANLVGATRETVTVTLRALQEEGIVTLGNRHLYLV
                     DPERLAQVAQPL"
     gene            complement(9820..10131)
                     /gene="rhaM"
                     /locus_tag="PKN92_08880"
     CDS             complement(9820..10131)
                     /gene="rhaM"
                     /locus_tag="PKN92_08880"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HNZ48946.1"
                     /translation="MRNAYTMKLKPGNEEEYKRRHDAIWPELAEALKEAGISHYSIYL
                     DHATGTLFAFQHLTEDNTAATLPSLPIVKRWWAYMADLMETNQDNSPVELPLEEVFYL
                     E"
     gene            10333..11268
                     /locus_tag="PKN92_08885"
     CDS             10333..11268
                     /locus_tag="PKN92_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylase"
                     /protein_id="HNZ48947.1"
                     /translation="MYSRHTIHALVLSAFVLSLCVTVFSEEHVQQPLPFPAELVAFQP
                     CKDNPVFQAGKPGDWDENIRERGWILYEDGVYHLWYTGYRSGPDEPRLLGYAYSQDGM
                     HWERHPDNPVHREGWVEDMSVVKVGNRWYMFAEGAGDLTHLLTSDDRIHWEDHGALTI
                     LKTSGEAIEPGPFGTPVIWHEKGVWYLFYERDDIAVWLARSEDLKTWIHVQDEPVMNR
                     GPLAYDLDMIAMDQVIRHGDCYYAYFHGLYPGGKPREWCSAIAASRDLIHWEKYSGNP
                     LIRDDKSSPIVVQTEEGYRFYTMHPAVYLFLPAGR"
     gene            complement(11349..>12828)
                     /locus_tag="PKN92_08890"
     CDS             complement(11349..>12828)
                     /locus_tag="PKN92_08890"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNZ48948.1"
                     /translation="GADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGLGADLLES
                     YVEAIIAPLAVASIMFAANPGTTEFVRSFPFFVAAIGIFASIIGVMYVKLFAKNNPQS
                     ALMNGTYISAVLAIIGVFAYTWLRGEGFTLEGVAYGKFGPAIACVVGIIAGMVVGFVS
                     EYFTSGSYRPVQKLAEGCQTGPAIAVTNGTAVGMQSTALPVLVLAIAVMVAAHYAGVY
                     GVAIAALGMLATTGMVVAVDAYGPIADNAGGIAEMAHMDPKVRHITDNLDAVGNTTAA
                     IGKGFAIGSAAFAAIGLLSAFMLSAKVTEVSIADPHILAGLLIGAMMPFFFSSLLFGA
                     VGRCADTMIQEVRRQFREIPGLREGLEGVHPDSTTCVSIATQGAIKGMLLPGGLAIVF
                     PLIIGLSPLGSAGLAGMLVGAIATGVMLGIQTANSGGAMDNAKKYIEEGNFGGKGSEA
                     HKAAVVGDTVGDPLKDTVGPSINILIKLMCVIALVLAPLFAG"
BASE COUNT         2946 a         3435 c         3581 g         2866 t
ORIGIN      
        1 caggacatga ttacgctaca gatcccaatg cctgtcttcg tcctgacccg cttgtgtgcc
       61 aatgcggaga ttggcgttcc cagggggaga attgctatgt ctccggttca gctcttctgt
      121 gtgaggagtg tatccgtttc ggagcacggt gcgagctgtg ccacaatggt gggccggggg
      181 tttttgccgc agaagaggtc gaagcgtctt gcgccgtcct gatccgtccc ttttaatgtg
      241 cagctgctgt ccatgaaaac ggggccttta aaggcaacat caagcctctg cggcccttcg
      301 attgcggggc cgggcaaggc tgtgagacag cgcgccagac tccacatacc gtgaatgata
      361 tcccgcttga atccgaagag ttttgccatg atgcgggaaa tgtggatggg attgaaatca
      421 cctgtaatcc gggcgtacgt acgtcccatt ttcttaggga catgccagga agcggtgaag
      481 gcttcttcgc ccagttcgct gaaagccgac catggatggg catcgccgat ttctccgaat
      541 ttttttccgc ggaaaagata ggagttcaca ctctcccata ttacgcttcc gccggaagag
      601 accagcgtgg tcacgtccac ttccagtccg gcctttagaa tacgcgcatc gctgaaaaag
      661 catttcaaat ccaccggttc tgtttcactg atggcacggc gctgcagcat gtggctgcgg
      721 gcgtggacgg caccgaaggc ctttgccgga aaacgtttgt cggccagaag atgaatatgc
      781 atggcaaagg tgagcacatg cgggtacaga atggggaatg tgccgtcatt ttcaaatccg
      841 cagacatccc gatattgctg aatgcgcgct gccggatcca ctgccttgtt ccattgagct
      901 tccagttttg gcagtgcctc aataccctga taggctgccc tgccgggcat aaaaatattt
      961 ttataggcac ttaacagacc gggtctctgc tggaattgaa ggattgcagc catgtgactt
     1021 aactcctgta tccgaaatgg aatgataagg tcagcgataa cactcacctt gccgtttctc
     1081 aaaagatgct acaccactac gttactattt tagaatattg actctgcttc tcacaaataa
     1141 gtgtcacaga ctgccacgca tttagtttgg cacgctttcc ttcagttcgc cttctttcga
     1201 ctgttcccct tccgcttctt ccttctcgcc gatggcaaag agaaaagccc gggggtccag
     1261 gctgtcgtac ccgctcagag cgccccagtg gatggcatat atacccggtt cgagcggata
     1321 gcccggatag agttcaacca attgcccgtc gctgtcgtcc agaattcggg caatgagcgg
     1381 taatgccgtg tcggcgatca ggattttttc cccggcctct gcttcttttg ccggtttctg
     1441 ctcctgctcc tttttctttt cctctttaag agttaaaaag cggagtttgt gcagcagggc
     1501 attgtacggc tggactttat aggcgagaaa acggagtttc tcctcctctg gcgctttttc
     1561 cggatcttcc cagggcagga tcagcgtcgg ctgcacaggt gtgccgaaga tgggttcccc
     1621 tttgccgatg gtatatcggt tgattcgggg atgttccagt gcttcatagc gaagcgcctg
     1681 cctgtaaaaa acaccttcct ccgctggaag aggcataagc accacatccg gcatttcatt
     1741 gcttccgaag gcaagactat ctacggcgac ccgggcggag gtgtaccctg ttttataaaa
     1801 atgcagagat gcggcacctc gcacagtccg gattgaatag cttccgttgg cgttggagag
     1861 cgcttcttta tcctgccctt ccagatgcac cgaaacgccg ggcagcgttt caccagccgc
     1921 atcgtagatc gcaccacgta tgactgcctg ctgcgtgcat ccgctttcaa tcagcggaag
     1981 gaacataagt acagccgctg tcagaacgaa gcgaagcctt ttcagggtgc gaaccagcgc
     2041 tgtcataaat acctttcctg accggtaatt ctgttcatat aatgtccatt gcgaagcgct
     2101 gcttccagcg aatcgccctg gctatcgtcc tgctccacaa tataaaagcg aacacccgca
     2161 tttttcagat caggcagaaa atggcgccag ggaataatgc cttttccaag ttccgtgaaa
     2221 gtggcgggcc cgccgtcatg atcgggctgg tagtctttca catgtaccag cggaatgcgt
     2281 ccctgatact tgtccagcaa atcttcaaag ctgattcctg ccacaccagc ccagcccaaa
     2341 tccagttcca gctggagatg ttgaggatcc gccgttttca gaatcagatc cagaatgcgg
     2401 tctgtgccgt agcgtgtaaa ttcatgcgca tgattatggt agcacaagag atacccctcc
     2461 ttccgaaatg ccgcacccag ctcatcaagc agccgggcag catcgatcca acttgcacga
     2521 tcggggcatt gctcttcgcc cagccagggc acgacaatat aaggtgcacg cagtgttttc
     2581 gcctgaagca tgaccatttc aggctgcgcc gacagctctt caatacccac atgcatactg
     2641 actgctttga gcgctgcctg atccagaaaa gtcttgaatt cggcggcaga tttcccgtaa
     2701 agacctgcca gctccaccca tttatatcct gctttgcgaa gtggtttcag tgctttcatc
     2761 aggtctttct ggcagtgatc ccgaatggaa tacaactgca gtccataagg ataaaggctc
     2821 atggcgatct ccaggatttt tcctgcagcg ctgcaggaag atttgctatg ggtattgaac
     2881 tctccgactc aaaagccgta ccatcacaga agcaacaggg agtacaaaaa tgacagtaaa
     2941 atatttttca ggactcatgg cagcgctgat gctgctaccg gtctgtgccg gtgccgcatc
     3001 gctgcgtgcc gtgctgcaaa aagaacaaat tgacgtgttg gcacaggaac agctggttac
     3061 ttcctataaa gtaaacaaaa aacagaagta tccttatttc tttccggtca acggaccggt
     3121 atcccatgcc agtctgacca cggaaacttc agagcctttt ccgcatcacc gctcgctatt
     3181 tttcggctgc gacagggtaa atggcggaaa ttactggcag gacagccttc gcgcaggaca
     3241 aattgtatcc ttggagctga aggtggaaaa ggctgaaggg gaagaggttg tctttaccga
     3301 tacctgcgaa tggcggcagc ccggtaaggc gcctgtgctt cgcgattatc ggcagatcac
     3361 cgtcagcgcc ccttctgatt cggtgcacgt cattgatttt cagatccgcc tcgtgcctct
     3421 gacagcgctg catatcgaaa aaaccaatca cagcctgttt gccgcccgga tggtgcccga
     3481 actgagtgcg gaaaaaggcg gtgtgctgat gaatgcggcg ggcgatacca acgaagcagg
     3541 aactttcggc attgtatcac cctggtgcga ttacagcgga agccgtcagg gaaacacgga
     3601 agggctcgcc atttttcagc atccggaaaa tccatgctat ccggcacctt ggtttacccg
     3661 ggattacggc tttttctcgc cgacgcccct gtactggccc ccatccgatg acgggatccg
     3721 gctgcaggaa ggggaagaac tgctcctgcg ctaccgggtc atcctgcatg caggcacagc
     3781 ggaggaagcg ggcatccccg ctctttttga agcctatact gccggcgcgg cttcctgcgg
     3841 caattgaacg aaaggaaaat gcggattcat ggtagataaa aacagaaaaa aacaattctt
     3901 cgatcagtta aagagggatc agcgtgaacg ggatgccggg tatcgggcac aggcgcttaa
     3961 actgtttccc catatgtgcc agcgctgcgg aagagaattc tccggcagaa acctgcgtga
     4021 acttaccgta caccataaag acggaaatca cttcaataat ccgccggacg gaagtaattg
     4081 ggcgatgctc tgtctttact gtcacgacga tgaacatggc acgctggagc agcgggggat
     4141 tgctgatgcc ggcgattttg cactgaaaaa agatgattca ctgacccaca atccttttgc
     4201 acagctgaaa aagcgttttg cggaatcgga cggggaacag aacggccccg gagaagaacc
     4261 atgaaaatcc gcatcctttt cgctgttacg ctgctttgcc tgctcacggt acacaccgga
     4321 acagcagccg ccgctgaagg cggaggcaca gcgctttttt ttgaagatcc tgcctttccc
     4381 gccgatgagc gcaccgtgcg ctgcctgaaa gaactgctga cgctggaagg ctatgcgctc
     4441 atgcctgtga atgcggaggc tctttgcagc actgcggaat tgacggcggc acacaatacc
     4501 ctgctcgtgc ttgccgatgc ggcacgcctg cccatggcgg caatcccccc agtaacggcg
     4561 tacctgaatc agggcggcaa tttgcttgcc ctcaatgcgc ctctgtggca ggagcggctt
     4621 acccgttttg gggatgagtg gctcagcctg gaagcatacc ggcggcgtat ggcaggtacg
     4681 gcaccgtctc actgttttct tgatttccgt gcggaatcgc tcgcatcctg gcaccgcagt
     4741 gcctaccgca tggagaccga tacacgcttt tctcttgttg tcgccgatga gctgggcgaa
     4801 ggtaaaaaag cgttgcttgt cgatatgccc gatatggaag gctgggactc cgcctgccgt
     4861 tctttcgaaa catcgcctta tccgccggga catgtcatga ccgtctttac tgcaaaaggc
     4921 ggtccccgga caacccggct tgccgtggaa ctgcgggaac gggacggatc acggtggatg
     4981 gcaacagtgc ctctctcccc gcaatgggag cagtacgtgc tcctgccgga agaatttgag
     5041 ttctgggaga gtgtgtatgc ccggaaggat acccgcctca atcctgccaa tgtggagatg
     5101 atcgctttta cccttgccta ttcccatacg gggcatctcc atggacagca cagttttgcc
     5161 gttgctgacg taggctccgc cccgctcgac gagaagtaca gagctgcacg caaggagatt
     5221 cccgtgcctg cttttgagat tctggcgccg tcctacaaat tttatcccat gagtgatgtg
     5281 acagcgctcg tgaccgccga ggacggactc ttgcctgccg gtgccccggc gcctatgccc
     5341 cgtgccatga tgagtatgca tccgcgaccc gaggcaggcg gttttgacaa agggcggcac
     5401 tggcgctggc ttcccctgtt ggaagccaga ggctccgatg gcacggtgcg cggctatcct
     5461 gccgccatgt tctttcagga cagcacttta tgtgccggag catgccgggc atcctttgcc
     5521 attgatgacc cgggctggta cgtttcttcc gccgggaaag aactgcttcg ttctgtgatt
     5581 cagcggatgc gcatgccgct ttttctgctt gacggcggcg ccgactgtta cacagccttt
     5641 gaggatcaga cggtacaggt gggcgctact gtagcgaacc gaagtggcgg agacgaagcc
     5701 gttcaactgg aatttgcgat aaaagataaa aacggctttc acaccgaaaa acgcaacatg
     5761 caaacagtga tccctgccgg aaaggaaatg gcagtcgagt cttctctcat gactttggag
     5821 ggaagagaca tgcctgttga gatcagtgta cggctgcttc agaaaggtga agtactggac
     5881 gaagcgcatc acatgctcca cctctggcgc ccgtctgctt cgccgagttt tgttgctgcc
     5941 gggaaagggc gctttgatct gaatgggaag cgctggattc cccacggggt caactatatg
     6001 ccttcttcgg gcatcggcgt ggaagataga acctattttg agaagtggct tggtgccgat
     6061 gcctatcacc cccgtgttat ccagcgggac cttgaacgct gcgtcgccat gggctgcaat
     6121 gcgctgagca tttttattta ccatgaatct ttgaagggac ataacctcct tgatcttctt
     6181 cggcgcatgg acgatctggg catgaaagcc aatctgtcgc tgcgcccggg aacacccatg
     6241 gacttcaagt gggatctcat caaagagctc attgaagtgc accgcctgag tgaaaatgat
     6301 accgtcttcg cttatgatct tgcctgggag ccccagttca gacctcacga gcgcgaacct
     6361 ttcaataagg actgggaagc gtgggtcgtt gaacggtacg gaagcattga gaatgccgaa
     6421 aaagactggg gcgagccttt tctccgggac gaaaacggcg cccctctgaa tattaccccc
     6481 gaaatggttg gcagagcagg gcagggcgcg ccgcgtatgg cggcataccg acgcttcctc
     6541 gatacccttc tttttgaaaa atacaatcag gcacgaacac tggttcacaa cattgatccc
     6601 aatcatcccg tcagtttccg catgagtatg gcgggagatc caacggattg gcagacagat
     6661 gcgctcctct atgacttcgc ctatctgggc ggggcggtgg atattttcga gccggaggca
     6721 tacggcagga taggcgactg ggaaaaagtg aagcccggcg cctttacttc cgcctatgcg
     6781 cgttgggcaa atccggcgct gcctgtatta tgggctgagg caggcgtgca tgtctgggac
     6841 atgagttcta tgaagcaggg cgaggacatg ctccagtttc aggcacagta tttcgacgat
     6901 ttctaccgga tgcttcttat gagccgggtg gacggcattt tctggtggtg gtatcccggc
     6961 ggataccgat ttgacgagaa gagcgactac ggcatcatta atcctgatgg aaccgacaga
     7021 ccgtcaacca aagtaatccg aaaatatgcg cccctgatca gccagatgga gactttgcct
     7081 cctgtagatg agtggctgct ttttgacagg gataaacata acaacgggct taccggtatc
     7141 tacagtgaat tgaaaaaaga cttctgggca aatatcgaag ccggcaaagt gcccggtctt
     7201 cgcacctcag gcaccggaac cgatacgcgg acctgtcccg cattggctgt gggcaatacg
     7261 ccgctcaatg gaaacaatcc gcccaagtat atggacagtg tctttgatca ggtcgcccgg
     7321 aaagatgaac agggcggatg gataccgttg aaaaatagag cgagtgtttt tcttccgcag
     7381 acggaagagc tgaatctgca gatctctctg acgaatcttg gtgaagctgc gctggttcca
     7441 ccggatgaaa atcggaacca ggcgggtgac gtttatctta ctgtgagcgg tgccctgtcg
     7501 ttccgtgttc ctatcacaca ggacgtgggt cgttttcaaa gtctgactat ggagctgtcc
     7561 ctcacggatg caatacggga tcagttgaaa gcgagcggga cgatccgttt gcagatggaa
     7621 gcccgggatc gcagcgcatt cggctcggtc tttgaattgc agttgaaaaa aatgcagcca
     7681 taagggaggg caaggatgaa aaaggtaagt cgtcgtgaaa tgctttgcgc catggcggcc
     7741 ggtacaacgg tggcactgct tccctcatat ctggcggcag agcagaaagc aaaacgcccg
     7801 aacattgtgt tcatccttgc cgatgatctc ggctacggcg gactcggctg ctacggtcag
     7861 aagaagatca agacgccttg tatcgaccgc atggcggcgg aaggcatacg tttcacccgc
     7921 gcctatgcgg gcagtacagt ctgtgcgccg tcacgctgcg cgctgatgac agggctgcac
     7981 accggacact gccggatccg gggcaatcat tcattggtcc ggcgtgatgc gctggcgttg
     8041 cttcccgacg atgtgaccgt ggcggaaacg cttcaaaaag ccggataccg tactgctctg
     8101 atcggcaaat gggggttggg cgaaatcggc tcttccggtg cgccgcgaaa taagggcttc
     8161 gatgaatttt tcggctatac cgatcagggg gaagcgcata attactaccc tgaatggctc
     8221 tggcgcaatg aagagaaaga gcccctgccc ggcaatgtac tggaaatgcc ccgggtttcc
     8281 aaagagcgga caacctacag ccatgatctg tttacaaagg aggcgctttc tttcctcggc
     8341 aggcagaacg gggaaaagcc ttttttcctg tatcttgcct ataccctgcc ccatgccaat
     8401 aacgaggcga cacgggtacg tgagcacggc atggaagtgc ctgacgaagg atcctatgcc
     8461 ggagaagact ggccgccggc ccagcgttcc catgccgcta tgatcagtcg cctcgaccgg
     8521 gacgtgggca gggttctgga tttactccgt gaaaggggac tggctgaaaa cacgctggtc
     8581 ttttttgcca gtgataacgg gccgcataaa gagggcggtg ccgatcccgc attttttgaa
     8641 gacagcggcc ctttccgggg ctttaagcgg gatatgtatg aaggcggcat ccgggtgccc
     8701 gccattgcct ggtggcccgg tgtgatttcg cccggacagg tgagcgatca ttgctgggcg
     8761 ttctgggatt tccccgtcac cgcatcaagc cttgccggtg tggaggcgcc gtccggagac
     8821 ggtatttccc ttcttcctct tcttaccgga aaagggcatc aggaaactca cgaggttttc
     8881 tattgggaat ttcacgaagg cggatgcagg caggcagtcc gtttcggtga atggaaggcg
     8941 gtgaagccgg gtaaaggaga gaagctcgag ctgtaccgtg tggaagagga tccgggcgaa
     9001 gagaacaatt gcgccggtct ctacccggaa aaagtgaaag aagctctgac ctatctccgg
     9061 cgtgaacgat cggaatctga ggacttcccg cgtcgatgat cagagcggct gcgccacctg
     9121 tgccaggcgc tccggatcca ccagatacag atgccggttt cccagcgtta cgataccttc
     9181 ctcctgcaga gcacgcagcg tgaccgtgac ggtttcacgg gtggctccca caagatttgc
     9241 cagttcctga tgcgtaagac gcagaggacc gttgtcttcc cgctccgcct cctgtccgga
     9301 caactccagc aggcggcagg cgatgcgctg gcgtacctcc ataaagagca gatcgctgag
     9361 gcttttttcc acgtccagag cgcggcgaca gcagtgcctg gcaagagcca ggctcaaagt
     9421 gccttcttcc tgcaccactt gaaagaagtc atcatagcga accagcgtta acactgtatc
     9481 ggttagcgct tcggcataat ctttccgctg agatcgatcc gcaagccctt cttcgccgaa
     9541 cagatcgccc ttaaaaagat gccggtaggt gagttcccgg tgcctttcag ttacatgggt
     9601 gattttgacc ctgcccgagc gaacccagta cacatagtca cagcgatcat cagggaaata
     9661 gatgatctgc ctcggatcat agcgccgatg ccaggcgaag gtgtccagcc gggctgaaat
     9721 ggacggatcc agacccagaa agaatttttc ctctgccaac gacggcaaag tcagtcgtgt
     9781 ggtcgtacgc atgctgtaaa ttcacttgac agaattgagt cactcaagat aaaaaacttc
     9841 ttcaagcggc aactcaacag gcgagttgtc ctgattcgtt tccataaggt ccgccatata
     9901 tgcccaccag cgttttacaa tcggaagcga cgggagggtg gctgccgtat tatcttccgt
     9961 gagatgctga aacgcgaaaa gggttcccgt tgcgtgatca aggtaaatgg aataatggga
    10021 gatgcccgct tccttcagcg cttcggcaag ctcaggccag atcgcatcat gacgccgttt
    10081 atattcttct tcattgcccg gcttcaattt catagtatag gcattgcgca tggcggttac
    10141 ccttgatgct gacgacttaa aaaagatcaa tattccgacc ccacaagtat agcatgatgc
    10201 ccgatggcac gaaaaagcct tttacgcgag agaatccagt gcttgcgctg ccaccggaga
    10261 aaaatgcgcg gcaggctctt ttcgtggtat ggtagattcc cgtgaacctg taaaaaaagg
    10321 agtacctccg atatgtattc gcgccatacc attcatgctc tggttctgtc cgcttttgtc
    10381 ctgtctctct gtgttacggt tttttcagaa gagcacgtac agcaacccct tccttttcct
    10441 gccgaactgg ttgcatttca gccctgcaaa gacaatcccg tatttcaggc gggcaagccc
    10501 ggtgactggg acgaaaatat ccgggaacgg ggctggattt tgtatgaaga tggtgtgtat
    10561 cacttatggt atacgggcta ccgcagcggt ccagatgaac cgcggcttct gggatatgcg
    10621 tattcgcagg atggcatgca ctgggagcgc cacccggata acccggttca tcgggaaggc
    10681 tgggtggaag atatgagtgt tgtaaaagtg gggaaccgct ggtatatgtt tgcggaaggg
    10741 gcgggcgatc tcacccatct gctgacctcg gacgaccgta ttcactggga ggatcatggc
    10801 gcgctgacca ttctgaaaac cagtggtgaa gcaatagagc ccggcccttt cggcacgcct
    10861 gtcatctggc atgaaaaggg cgtctggtac ctcttttacg aacgggacga tatcgccgtg
    10921 tggcttgccc ggtcagaaga cttgaaaacc tggattcatg tgcaggatga accggtgatg
    10981 aaccggggac ctctcgccta cgatcttgac atgatcgcca tggatcaggt gatccggcac
    11041 ggggactgtt attacgccta tttccacggt ctgtatcccg gagggaaacc gagagaatgg
    11101 tgttcagcta ttgccgcttc ccgtgatctc atccactggg aaaaatactc cggtaatccg
    11161 ctgatccgcg atgataaatc cagtccgatt gtggtgcaga ccgaagaagg atatcgcttc
    11221 tataccatgc atccggcagt atatctcttc cttcctgcag gcagatagaa ccggtttcgg
    11281 ggatataaaa aatcacgccg cccagttaag agcggcgtga cagaaatcta ccggtaaatg
    11341 gatgaggctc agcccgcaaa gagcggcgca agcaccagag caataacgca cataagcttg
    11401 atcagaatgt taatggaggg gccgaccgtg tctttcagcg gatcgccgac cgtatcgcct
    11461 accacagcag ccttatgggc ttcagacccc ttgccgccga agttgccttc ttcaatgtac
    11521 ttcttggcgt tgtccatggc accgcccgag ttggctgtct gaatgccgag catgacgccg
    11581 gtggcaatag caccgacaag catacccgca agaccggcag agccaagcgg ggacagaccg
    11641 ataatcagcg ggaagacaat ggcaagaccg ccgggaagaa gcattccctt gatggcgccc
    11701 tgtgtcgcaa tgctgacgca ggtggtgctg tcgggatgga cgccttccag accttcgcgc
    11761 agtccgggaa tttcacggaa ctgacggcgc acttcctgaa tcatcgtgtc cgcacagcgg
    11821 ccgacagcgc cgaaaagaag ggaggagaag aagaaaggca tcatggcgcc gatcagcagc
    11881 ccggcaagga tgtgcgggtc ggcgatactg acttccgtca ctttggcgga aagcatgaaa
    11941 gcgctcaaaa ggccgatagc ggcgaaggcg gcagagccga tggcaaagcc tttgccgatg
    12001 gcggcggtgg tgttgcccac agcgtccagg ttatcggtga tatggcgaac cttgggatcc
    12061 atgtgcgcca tttccgcaat accgcccgca ttgtcagcga taggaccgta agcatcaaca
    12121 gcgacgacca taccggtcgt ggcaagcatg ccaagcgcag caatggcaac gccgtagacg
    12181 ccggcgtaat gggcggcgac catgaccgca atggcaagaa caagcacggg cagggccgta
    12241 ctctgcatgc cgactgcagt gccgttggta acggcaatgg cgggacccgt ctggcagcct
    12301 tcagccagtt tctgcacagg acggtagctg ccggatgtaa aatattcgct cacgaaacca
    12361 acgaccatgc cggcaatgat accgacgacg cacgcaatgg cgggtccgaa tttaccgtaa
    12421 gcaacgcctt caagcgtaaa gccttctcca cgaagccagg tataggcgaa tacgccgata
    12481 atggcaagga cggcactgat ataggtgccg ttcatgagcg cactctgggg attgttcttg
    12541 gcaaaaagct tcacatacat cacgccgatg atggaggcaa aaataccgat ggcggcgaca
    12601 aagaagggga aggatcttac aaactcggtt gtgcccggat tggcggcaaa catgatgctc
    12661 gccacagcca gcggcgcaat gatggcttcc acataggatt caagcagatc ggctccaaga
    12721 ccggcaacgt cgcccacgtt gtcgcccacg ttgtcggcga tcactgcggg gttgcgcgga
    12781 tcgtcttcag gaatacccgc ttccacttta cccaccagat cagcgccc
//
LOCUS       DAOPWB010000088        12734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5021_length_12734_cov_6.337960, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000088 DAOPWB010000000
VERSION     DAOPWB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5021_length_12734_cov_6.337960"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..3629
                     /locus_tag="PKN92_08895"
     CDS             252..3629
                     /locus_tag="PKN92_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ48949.1"
                     /translation="MNFTENQKKAIQANRPVVGVSAGAGSGKTAILVERIVYLLSNHE
                     LWGGVQPGLNRIAAITFTDKAAAEMKARLRRRFKQEVQGCAHPDRMLFWRSMEHQVES
                     AHISTIHSFCAAVLRENALHFNRDPDWKVLKEVEAARAIEGAITDTLNALLEQNDPSA
                     CRLALQMSRQEIKSVLKKMLLNRWDYLAVWEDMPVESADQLFDRWEKSFPLLYRSFLQ
                     KLKHAPQLDYFLSALQEFDGASLNPFDVREKTRASYIRALTAIKLGQEDSEIFLNEIN
                     RDFLDLRHSSKGWDKELFKALGVVLGQTRDWIKEQVLCPERLYDSMMRSAENTFDFWV
                     LFRKANEKVQQIRNATASLDFDDMINETLYFLKSDPDLCRRIALSYSFLLIDEFQDTD
                     SRQLSIARMLADVAGGPSLFYVGDAKQSIYLFRGADVSLFNGEMGRDSHAVSMQDNFR
                     TLPDVLHFVNDFFVRSNLLSSVEAYGGMAPQRTPLKECRVECFITESKENVEMRRRAE
                     AAFIAGRIAEMCGGETAVQVRCSNDRDMRPASYDDIVLLFRRGSQIHFFESALRAARI
                     PYNRIAGEGFFKRREVLDVIAMLKWVLDPWDEEAMLGVLRSPFVGLSDEDLMSLARTE
                     KGLAASFHDDTSDPLLKHPERLAQGRLLFRDLYRRREWPPARWLDRLLSLTCYESVLM
                     DHRMGLQRVSNLRKLRKMAENFSQTDLDVLTAFVEYLEEQSVRELKEGEAELQSKGMG
                     AVTIMTVHKAKGLEFPVVFLPDLDVGTRSDTRDKIVFFRDNDFAVQFSGGRRGFEDSG
                     FYQLLRRINRLDNQLELARILYVAMTRARDYLVLCSQADATLFTWAGILNRMYALPDR
                     ADGEVLCDDNWQLRIRREQPIQPYITPERKEYPVPDEDRILARLQSPANEEQAKRCIS
                     VSALLDIMGFGEDQEEAKRNEFREASEFSEGEDSSALIVKRAAAMDRGTLFHRIFELW
                     DFKKDLLPPLDELIHESGLDFREAKEFRATMEEAIAVFRRSDSWRLFSGAGSIEREMP
                     FLLKLDDCLLKGVIDAVIDQRIIVDYKTGHAHPDSRERYRTQVMLYGAALWKIRGILP
                     AKGLLWYVDQGFSEEFSICPADLEEVMRCVSLNLQKC"
     gene            3855..6053
                     /locus_tag="PKN92_08900"
     CDS             3855..6053
                     /locus_tag="PKN92_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48950.1"
                     /translation="MIRRIFKGEPEAYRLLIERYQPMAYAIAMANSGSVFLADKITAA
                     AFESGYGRLISLTDPRKLGQLMCGLAHQESENLLSRRATNWNIPRTRLEVAPVDLQWI
                     QTELIEPLMEELGSFTLHERMGILLNTFAGLNARQIADVLKIDPKDAAEDLARTRENV
                     ESALLKEVRAALYPEVNSRERLGSIISTVGGRDAADKAMSKSRLGRRKSRKVPLVLTA
                     CTFIVIGISAYFGYHAFNGAVLEQQPNIPSSPAPTKESSQEPATTEPPANRPAPVNYS
                     LIGRVVDNRFREDGVAGVTVKVQDKEAVTDSYGAFELRGVARGEHRVSISYGDEILKS
                     DVRLHTNEKNLPIAVDLTDSVPTRFTMIGQVTDRATGQVLKDIEQACIKENSSVLFPF
                     ILDLFKKERHEDGRVQIRLLTPGEYTAWVRAKGYAPLAVSFVIDENWDPDRVFEFPLY
                     RAALLEGVVYSASELSISGAGIMPRDGSRFNLVYSRFSYGNTDSKGHFALYDLPLGVH
                     NLLIGSEEHGSARAIVVLEAGKTTTIRAQVPHASSFSGDITLYGLPVKFKELRRRQGG
                     NNVDLLKNVVYLSPGQYELPFTPEAVHFWGSVEPLPGDTWFERRIEQESTVDPSQPVW
                     VDFNFNDGKGSVQGNVSFQGNIPRSAFAEVTLEKEDSRELEKIYYDLGTSGSYRAENL
                     PLRAGSVTVYASDKALSRDDFWQNRSLLNKKTASFSLSEGKPGAQLNFNL"
     gene            6181..6969
                     /locus_tag="PKN92_08905"
     CDS             6181..6969
                     /locus_tag="PKN92_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48951.1"
                     /translation="MVFFSAFIFVASAFMGEVVAPSPDTGIAEPAVLSEKAVIVLLQG
                     MGRGRASLWVLDTRLQSEGYETLNFPYVAHSRSIEEMAEQFHDFLDEKLDNRPYHIIA
                     HSLGTVVVRAAFGKELPPGLGRVVLIAPPNKPTELLRVLKENPVYQWFTGSSGQQVAS
                     DEFYEKLPVPSVEFGIIAGDRGHEFTLRGPNDGVITVESTRLPGMSDWIVTPHAHNFL
                     ANSRQVAALCLSFLRQGSFDRALTATDLPRSEEETRDSDDGRES"
     gene            6977..7933
                     /locus_tag="PKN92_08910"
     CDS             6977..7933
                     /locus_tag="PKN92_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNZ48952.1"
                     /translation="MNILIMGAGALGSVAGGFLANKGHHVSLIGRRLHMDTVASQGLQ
                     IRGIWGEHRVEGMHVLSKPEVLPSLSFDLVLMAVKSYDTATAADLIAPYVSDNTLICS
                     YQNGLGNAEVLAQKFGWNRVIGARVIFGARVYEPGAVEVTVMAAPTALGAYPGGPSKE
                     RIAVIVKAMADAGLPTVMTDEIRTLLWEKVAYNCALNPLSALLDVPYGALAESFYTRT
                     VMAEVIEELYATAHRLRVPLKPDCSDAWLDHFYQNLLPPTAAHYASMREDLLRGRRTE
                     IDALNGAIARFGNEQGISCPTNALLTALVHAREKTPACPSSL"
     gene            8042..9277
                     /locus_tag="PKN92_08915"
     CDS             8042..9277
                     /locus_tag="PKN92_08915"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HNZ48953.1"
                     /translation="MRLLPMLMNALLLSALTVTGAAAALNGTTFKIQGGDRDQTHIPI
                     EVAVDCDEVPAFVTVINKETQVRYPATVRNGQLCFIADLVPAQKELSAVVETGERSAD
                     MPWGVRLSRKSGADQVEVFIDGKHFTTYHYGPEWKKPFLWPVNSEGGVGVTRDFPAEL
                     ESTPRKDRDHPHQKSLWTAFGEVNGQDLWAEGTGSGTQKTVEVTFGSGDAYGWISARN
                     VWEDKDGKALLDESREYRFYAAPESGRLIDVKVTFAAAYEDVLFSDTKEGGIVSLRMR
                     PDICHSSAVITNALGDVGENTAWGKPSPWCDFSGEIPGVGWRGIAVFDHPDNLRYPTC
                     WHVRSYGLMGANPFGYSYFNEKEYNRGLIPEKGDYTMEKGAALVFRYRVLIHSGTVAE
                     AGTADRYSAYSHPPALLPN"
     gene            10193..12700
                     /locus_tag="PKN92_08920"
     CDS             10193..12700
                     /locus_tag="PKN92_08920"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNZ48954.1"
                     /translation="MSTHCHPSTTAIKRILTTTWSLLLVGLLLMPIAAFAQTSVVFDS
                     PGATQFDVPATITKITVEAWGAGGRGSARTTGNNVALAGGGGGAYSRSEFTVTPGDTF
                     DLYVGAGATNTSAGGDSWFGSVSTVMAKGGGSVANNSNTPGAGGLASAGVGTVKFNGG
                     TGALGTGGSFGGGGGSSGGTAAAGVYPDGTTTVRTGGTAPAGGGDGGAGAASGVVGQE
                     GAAPGGGGGGGYRAGSGTTQAPGNGAPGRVVVTYDCAISSVTFDAVADPDDEVYRCAA
                     AETETYTVTAPGATTLTYSLDAASLAGGNTIDSATGTVTWDAAWVGFSTVTVEASSSG
                     CALTANLLVHTVALVQAPVFDAGSTVQRCRAAESIVYSATAHHADTLVYSLDAASLAG
                     GNTIDSATGEVTFVATWSGTSTVTATATGCGTTKTADLTVLTGALFANDDYVVTLQGN
                     LAHFNVLDNDLCNIDASSVTLISSPIGGFVQVGAGGEMTYVSFGAFEGLDQFTYQVCT
                     PAPVQCKQATVYIQVNPALDDPCYVANKASLFYLPYPENETHLRQSLLSAGSQAMSGG
                     SNGGTDVRTVVSILVPYPGTRIVYDHWEDGYEADSNFPVQSTTEVWGDGDLTNGVAPG
                     YPTDFIPGGGYLIIDNTFPWNRSIVTIVPDGKDKISSSSSIIVSKVSGDAARFAVQNV
                     KTNVSEESYAGQHFVLPFGENVTDGGTSAFRYTGMFVRAYENGTVVELDYNGDGTNDV
                     TSPTLDEGDVWFYQGTASSPGVAGDVNNSNDIKAGARVTASKDVSVDLVFGGIDNYGT
                     RNIAIYPSNYYGDKYYSPVYSTNTDALAYAFCASPHF"
BASE COUNT         2935 a         2971 c         3591 g         3237 t
ORIGIN      
        1 ggtgctgttt tcggcgattt tttccatgtt gattatgctg tacactgtga ggtgggtcac
       61 tttatttata tggtgtatac tggattttcc ttgccacgct ccttaatact atacacagta
      121 ttcgctcatc agaaatcgcg gttaattggt gtaatgtctt ggtgagaggt tataataaaa
      181 ttaattgact ttataaaaga ggccggataa tatagaaaac ggaaggctgc ccattttcag
      241 agggagatat tatgaatttt actgaaaacc aaaagaaagc aattcaggcg aaccgccctg
      301 tcgtcggagt ttcagcggga gcgggctccg ggaaaacagc gattctggtg gagcgaattg
      361 tttatctttt atcgaaccat gaactctggg gtggcgtcca gcccggactc aaccgtatcg
      421 ccgccattac ctttacggac aaggcagccg ctgaaatgaa ggcgcgcctt cgtcggcgct
      481 ttaaacagga agtgcaaggt tgtgcacatc cggatcggat gcttttctgg cgcagcatgg
      541 aacatcaggt ggaaagtgct catatttcga ccattcactc tttttgcgcg gctgttttgc
      601 gtgaaaatgc gctgcatttc aaccgggatc ctgattggaa ggtgttgaaa gaagtagaag
      661 cagcccgtgc catcgaagga gcgattacgg acacgctgaa cgccctgctg gaacagaatg
      721 atccgtctgc atgccgtttg gctctgcaga tgagccgtca ggagattaag agtgttttaa
      781 aaaaaatgct gttgaaccgg tgggattatc ttgcagtatg ggaagacatg cctgtggaga
      841 gtgcggatca gctgttcgac cgatgggaaa agtcttttcc gttgctgtac agaagttttc
      901 ttcagaagct gaagcatgcg ccgcagcttg actatttttt atcggcattg caggagtttg
      961 acggtgccag cctgaatcct ttcgacgtgc gggagaagac gcgggcttcc tatatacggg
     1021 cgctgacagc cataaaactc ggacaggaag acagtgagat ttttctgaat gagataaatc
     1081 gggactttct ggatttgcgg cattcatcaa agggctggga taaggagctt ttcaaggcgc
     1141 tgggtgttgt tttgggtcaa accagagatt ggatcaagga acaggtatta tgtcccgagc
     1201 ggctttatga ttctatgatg cgcagtgcag agaatacctt tgacttctgg gtgcttttcc
     1261 ggaaagccaa tgaaaaggtt cagcagatca gaaatgccac agcttctctg gactttgatg
     1321 atatgatcaa tgaaacactg tatttcctta agagcgatcc ggatctttgc cgtcggatag
     1381 ccctgtctta ttctttcctg ctcatcgatg aattccagga tacggacagt cgccagctgt
     1441 ctattgcgcg catgctggcg gatgtggccg gagggccgtc gcttttttac gtcggtgatg
     1501 cgaaacaatc catttatctt ttccgtggtg ccgatgtttc gttattcaac ggagaaatgg
     1561 ggagagattc ccatgccgtg tccatgcagg ataacttccg cacccttccg gatgtgctgc
     1621 attttgtgaa tgactttttc gttcgtagta atctgctgag ctccgtggaa gcatacggag
     1681 ggatggcgcc gcagcgtact ccgttgaaag aatgccgggt ggaatgtttc ataaccgaat
     1741 ccaaggaaaa tgtggagatg cggcgccgtg cggaggcggc attcattgcc ggtcgcatag
     1801 ctgaaatgtg cgggggcgaa acagccgtgc aggtgcggtg cagcaacgat cgggacatgc
     1861 gtccggcatc ttatgacgat attgtgctcc ttttccggcg tggttcccag attcactttt
     1921 ttgaatcagc actgcgcgct gcgcgaattc cgtacaatcg catcgcgggc gagggttttt
     1981 tcaagcgtcg cgaagtgctg gatgtcattg ccatgctgaa atgggttctt gatccctggg
     2041 acgaagaggc gatgcttggc gttctgcgca gtccttttgt cgggctgtcc gatgaagatc
     2101 tgatgtctct tgcccggaca gaaaaaggac ttgccgcttc ttttcatgac gacacatccg
     2161 atcctttgct gaagcatccg gaacgtctgg cgcaagggcg cctcctgttc cgggatttat
     2221 accgccggcg cgagtggccg cctgcgcgat ggctggacag gcttttatcg ttgacctgct
     2281 atgagtctgt gcttatggat caccggatgg gtctgcagcg ggtgtccaac cttcgtaagt
     2341 tgaggaaaat ggcggaaaat ttcagccaga cagatcttga tgttctgacc gcatttgtgg
     2401 aatacctgga agagcagtct gttcgtgagc tgaaagaggg agaggcagag ctgcaatcca
     2461 aagggatggg agcggtcacc atcatgaccg tccataaagc gaaaggactg gagtttcctg
     2521 ttgtctttct tccggacttg gacgtcggca cacgcagcga cacaagggac aagattgttt
     2581 tttttcggga taatgacttt gccgtacagt tcagcggtgg tcgccgcggt tttgaagaca
     2641 gtggtttcta tcaactgttg cgccgcatca atcgattgga caatcaattg gagctcgcac
     2701 gtattcttta tgtcgccatg acccgtgccc gggactacct tgtgctgtgc agccaggctg
     2761 acgcaactct tttcacctgg gcgggcatac tgaacaggat gtatgctctg ccagacagag
     2821 cagacggaga ggtgctttgt gatgacaact ggcagcttcg catacggcgg gagcagccga
     2881 ttcagcctta tataacacct gagagaaagg agtatccggt tccggatgaa gacaggattc
     2941 ttgcccgtct tcagagcccg gcgaatgagg agcaggcaaa gagatgcatt tctgtgtcgg
     3001 ctctgctcga cattatgggc ttcggcgagg atcaggagga ggcaaaacgg aacgagttca
     3061 gagaagcatc cgagttttcg gaaggagaag acagcagcgc tctgattgtg aaacgtgccg
     3121 ccgccatgga tcgcggaact ctttttcacc ggatttttga attatgggat ttcaagaaag
     3181 acctgctgcc ccccctggat gaattaattc atgagtccgg tcttgatttt agggaggcga
     3241 aggaatttcg ggcaactatg gaagaagcga tagccgtttt tcggcgctca gacagctggc
     3301 gacttttttc cggtgcaggc tccatagaaa gagaaatgcc ttttttgctg aaacttgatg
     3361 attgcctcct caaaggtgtg atagatgctg taatagacca gcgcattatt gtcgattata
     3421 aaacagggca tgcgcatccg gacagccggg agcgatacag gacgcaggtg atgctctatg
     3481 gtgcggcact ttggaaaatt cgtggtatac ttcctgcgaa aggtctgttg tggtatgtgg
     3541 atcagggatt cagcgaagag ttttccatat gtcctgctga tctggaagaa gtgatgcggt
     3601 gtgtgtcgtt aaatctgcaa aagtgctgag ggtgtgtgat aaacaagaga tacagaagac
     3661 tgtttacagg caacttgacc tgagcgacag tttaccgcat aattatatat ggagagggtg
     3721 gctgattttc agcccgcatt cttcagagcc tatggagacg agggtcgcgg taaaagtaat
     3781 acaaagcgct ggcagagcgc cggtaagggc ttgataacgt gcttcctttt tcacgtttta
     3841 aaaaagaagc ggtgatgatt cgccgcatat tcaagggaga accggaagcg taccgcttgc
     3901 tgattgagcg gtatcagcct atggcttatg ccatagcgat ggcaaattcc ggaagcgtat
     3961 ttctggcgga caaaattaca gcggccgctt ttgagagcgg ttacggccgt ctgatttctc
     4021 ttacggatcc gcgcaagctc gggcaattaa tgtgcggtct tgcacatcag gaatccgaaa
     4081 atcttctttc ccgccgcgcc accaactgga atatcccccg gacacgcctg gaagtcgccc
     4141 ctgtcgacct gcaatggatt cagacggaat tgatagagcc tctgatggag gaactgggtt
     4201 ctttcacgct tcacgaacgg atggggatac tgctgaacac ctttgccggt ctcaatgccc
     4261 gtcaaattgc cgatgtgctt aaaattgatc cgaaggatgc ggctgaagat cttgcccgca
     4321 cccgggagaa tgtggaaagc gcactgctga aagaagtgcg ggcagcactg tatcccgagg
     4381 tcaacagccg ggaacgtttg ggttcgatta tttctaccgt gggcggtcgt gatgcagcag
     4441 ataaggcgat gagcaaaagc cggttggggc ggcgtaaaag ccggaaagtt cctctggtcc
     4501 ttaccgcctg cacctttatc gtgataggca tttccgccta tttcggctat catgctttca
     4561 acggggcagt gctggaacag cagccgaata ttccttcctc tcctgcgccc acgaaggaaa
     4621 gcagtcaaga gcctgccacc acagagccgc cggcgaaccg ccccgcacct gtcaactatt
     4681 ctctgatcgg tcgcgtagtg gataatcgtt ttcgcgaaga cggtgtggca ggggtgacgg
     4741 tgaaagtaca ggacaaggaa gctgtaacgg actcctacgg tgctttcgaa ttgcgtggtg
     4801 ttgcccgggg agagcatcgc gtctccattt cttatgggga cgagattctc aaaagcgatg
     4861 tgcgtctgca taccaatgag aaaaatctgc ctatcgctgt tgacctgact gattcagtgc
     4921 ccacccgttt caccatgatc ggacaagtca cagaccgggc caccggacag gtactgaaag
     4981 atatagagca ggcttgcatt aaagaaaaca gttcggttct gtttcctttt atcctcgatc
     5041 ttttcaagaa agagaggcac gaagacggtc gggtacagat ccgcctgctg acacccggcg
     5101 aatatacggc ctgggttcgt gccaaaggat atgcacccct cgccgtgtct tttgtgattg
     5161 atgagaattg ggaccctgac cgtgtttttg aatttcccct ctaccgggcg gctttgctgg
     5221 aaggggtcgt ttacagcgcc agcgaactca gtatcagcgg agccggcatc atgccacgtg
     5281 acggttctcg ttttaatctg gtttattccc gcttttctta tggaaacacg gattcaaaag
     5341 ggcattttgc gctttatgac ttgccgttag gtgtacacaa cctgcttatt ggaagcgagg
     5401 aacatggttc tgcccgggcg attgtcgtgc tggaggcagg aaaaacaacc acgattcgcg
     5461 cccaggttcc ccatgccagt tcgttcagcg gcgacataac gctgtatggc ttaccggtta
     5521 aatttaaaga attgcggcgg cggcagggcg gcaacaacgt ggatttgctt aagaatgttg
     5581 tctatctgtc gccgggtcag tatgaactgc ctttcacccc tgaagctgtt catttttggg
     5641 gatctgtaga gcctttgccc ggggatacct ggtttgagcg gcgtattgag caggaatcta
     5701 ccgtcgaccc atcgcagcca gtttgggtag attttaattt taacgatgga aaaggcagcg
     5761 ttcagggcaa tgtttctttt cagggaaata taccgcgctc cgcttttgcg gaagtgactt
     5821 tagaaaaaga agatagccgg gaactggaaa aaatctatta tgatctcggt accagtggct
     5881 cttatcgtgc agaaaactta ccgctgcgag caggttctgt cactgtgtat gcttcggaca
     5941 aagcgttgag tcgggatgac ttctggcaga accgctccct tctcaacaaa aaaaccgctt
     6001 ccttctccct gtcggaaggg aagccgggcg cccagctgaa ttttaatctg taacgccagc
     6061 gttttcccga gcgctttttt ctgttcatct tctgccaatg ccgctgcgga ttgtttgtca
     6121 agaccgctgg cttgtgctct gctttcaacg ctatcgttgc ttccggatac cttcatttcc
     6181 atggtcttct tcagtgcatt tatttttgtg gcatccgcct ttatgggtga ggttgtagca
     6241 ccctcgcctg ataccgggat tgccgaaccc gctgtcctgt cagaaaaagc agttattgtg
     6301 ctgcttcagg gtatgggtcg gggccgtgct tctttgtggg tccttgatac ccgacttcag
     6361 agcgagggat atgagacgct gaacttcccc tatgtggcac acagccgctc cattgaagag
     6421 atggcagagc agttccatga ttttctggac gagaagctcg ataacagacc ttatcatatc
     6481 atcgcgcatt ctctgggcac agtggttgta cgtgccgcct ttggaaaaga actgcctccc
     6541 gggctgggca gagtcgtatt gatcgcacct cccaataaac cgacagaatt gcttcgggtt
     6601 ctcaaagaga atccggtcta tcagtggttt acaggaagca gcggtcagca ggttgcctcc
     6661 gatgagttct atgagaaact gccggtcccg tccgtggaat tcgggattat tgcgggcgac
     6721 cggggtcatg aattcacgct aaggggccct aatgacggtg tgatcacggt cgaaagcacc
     6781 cgccttccgg gaatgtcgga ctggattgtg acaccccatg cccacaattt tctggcgaac
     6841 agccggcagg tggcagcatt atgcctttcg tttttaagac agggctcttt tgaccgggca
     6901 cttacggcga ctgatcttcc ccggagcgaa gaggaaactc gggactctga cgacggcaga
     6961 gaaagctgaa aaagcaatga atatactgat tatgggtgca ggagcgctgg ggagtgttgc
     7021 cggaggattt ctggcaaaca aagggcatca cgtcagtcta atcgggcggc gcctgcatat
     7081 ggacactgtc gcttcgcagg gtctgcaaat acgggggatc tggggggagc atcgggtaga
     7141 aggcatgcat gtgttatcga agcccgaagt gttgccatcc ctttcttttg atctggtgct
     7201 gatggcggtc aaatcctacg atacagcgac tgcagccgat ctgattgctc cttatgtgtc
     7261 ggacaacaca ctgatttgct cgtaccagaa cgggctcggt aatgctgaag tgctggcaca
     7321 gaaatttgga tggaaccggg taatcggtgc ccgggtcatt tttggtgccc gagtgtatga
     7381 gcctggcgcg gtggaagtga cggttatggc ggcaccgacc gcattgggcg cttatccggg
     7441 cggtccgtcg aaggaacgta tcgccgtcat tgtgaaagcc atggcggacg ccggactgcc
     7501 tactgtaatg accgacgaaa tccggacact cttatgggag aaagtcgctt ataactgcgc
     7561 attaaatcct ctttccgcat tgctggatgt gccttacggt gcgctggctg aaagtttcta
     7621 taccagaaca gttatggcag aagtgatcga ggaactttat gcgacagcac accgattgcg
     7681 cgtgccactg aagcctgact gttccgatgc ctggcttgac cacttctatc agaatttgct
     7741 gccgccaacg gccgcccact atgccagtat gcgggaggat ctcctcaggg gaaggcgtac
     7801 ggagatagac gcattgaacg gtgccattgc ccgtttcggc aatgaacagg ggatttcctg
     7861 tccaacaaat gcgcttttaa ctgctctggt gcacgcccgt gaaaaaacac cggcttgtcc
     7921 gtcctctttg tgagggcgga gcgccacatt ttttattgcg ttaacagcct tcttctgata
     7981 aactgaaggt ttacagtctg aaaccgatgg catgaccaga ctaaaaaagg agtattgcct
     8041 tatgcgtctt ttgcccatgt tgatgaatgc tttactgctg tctgcactga ccgtaaccgg
     8101 agccgcagcg gctctgaacg gaacaacgtt caaaattcag ggcggtgaca gggatcaaac
     8161 tcacatcccg atagaggtgg ctgtggattg tgatgaagtg ccggcttttg tcactgttat
     8221 aaataaggaa acgcaggtgc gctatccggc aacggtacga aacgggcagc tctgctttat
     8281 tgccgatctg gtgcccgcac agaaagaatt gagtgctgtt gttgagaccg gcgagcggag
     8341 tgcggatatg ccttggggcg tacgcctttc cagaaaaagt ggtgcggatc aggtggaagt
     8401 atttatcgac ggaaagcatt ttacgaccta tcattacgga ccggaatgga aaaaaccttt
     8461 cctctggccg gtgaacagtg aaggcggcgt gggcgtgacc cgcgattttc ccgcggaatt
     8521 ggagagcacc cccagaaaag acagggatca tccgcaccag aaatcgctgt ggacagcctt
     8581 tggtgaagtc aacggccagg atctctgggc ggaaggaacc ggatcgggta cacaaaagac
     8641 ggtggaagtg accttcggtt ccggcgatgc ttacggctgg atcagcgcac ggaatgtgtg
     8701 ggaagataaa gacgggaaag cgcttctgga cgaaagccgg gagtatcgct tttatgctgc
     8761 gcccgagtca ggcaggctga ttgatgtaaa agtcactttt gccgccgctt atgaagacgt
     8821 actgttcagc gacaccaagg aaggaggtat tgtttctctt cggatgcggc ccgacatctg
     8881 ccacagcagt gcagtcatta cgaatgcttt gggcgatgtc ggcgaaaata cagcatgggg
     8941 caagccatcg ccctggtgcg atttctccgg ggagattcct ggcgtgggct ggcgcggtat
     9001 cgcggttttt gatcatccgg ataatctgag gtacccgacc tgctggcatg tgcgcagtta
     9061 cggactcatg ggcgcaaacc ccttcggtta ttcctatttt aatgaaaaag aatataacag
     9121 agggctgatt ccggagaagg gcgactatac aatggaaaaa ggagcagctc ttgtgttccg
     9181 ctacagagtg ttgattcata gcggcactgt tgcagaggca ggcacagcgg atcggtacag
     9241 tgcgtacagt catccgccgg cgcttttgcc aaattaattt aaactgtttt gccttcgata
     9301 taagcggcag ccgtataacc accgggatac ggaagccgct tgttttatca cttccgcacc
     9361 ctttaaacat tggaggtaca tctgtttcgc ctatatgaag acactgaaga aatttctaag
     9421 aaacatttcg aaagacactc agtttgtaat gaaaataagg catttatcct gatatcaagt
     9481 agaaccataa aatgatgttt tctcacattt agtgtaaatg attttttgat gccaccaggc
     9541 ggcgccagaa tcagtgtaac taatgcaagt aaagggaatg cgacagccct ttaatcgcaa
     9601 aaattaaggg cgttccagcg catgaaaaag caggcataaa catgtgaaat cgtatgaata
     9661 ttaatctgat agcattcatg aggcctttat tgtaggaaat cgcccataaa caagacgata
     9721 atatttatag tgttttgcca ataatatagg atatgatata ttgtgcagac cgtgtgtgat
     9781 taaaagatgt catatttgcg taagtaactt ttatttctct tgcagttgaa aatattttgt
     9841 actttatggt aaaatgaatt catcaatgcg ggaaaggaac cttttgttaa actgaacgct
     9901 gttcagaaaa agaggcaatt ttacgattag ttttctttaa gggaataaac atcttttaga
     9961 aacgggtttg catggaagca atgcccgttg tgtgttaaat ttgagttttt tttgagtaag
    10021 cgttttggtt ttgattcgta ggagacgtct gctctgtgcc ccttgtaggg tattgggagt
    10081 ttctgtttta aatttgggag tttctgtttt aaataaagta tggatagccg ccatgcagcc
    10141 tgttcgggag acaggtgtga gaggcttttg gtgcaacagc aaggttgaaa agatgagtac
    10201 acactgccac ccctctacca cagcaatcaa aagaatcctt acaaccacgt ggagtctgct
    10261 tcttgttggg ctgcttttaa tgcccattgc agccttcgct cagacgtcgg ttgttttcga
    10321 cagtccggga gcgacgcagt ttgatgtccc tgccacgatc acgaagatta ccgtggaggc
    10381 ctggggcgca ggtggtcgcg gctcggcgcg aaccaccggc aacaatgttg ctcttgccgg
    10441 cgggggcggt ggtgcctatt cccgcagcga attcacggtg acgcccggcg atacctttga
    10501 cttgtatgtc ggtgccggcg caaccaacac ctctgccgga ggagactcct ggttcggctc
    10561 tgtcagcacc gtgatggcaa aggggggcgg cagcgtagct aataacagca atacacctgg
    10621 tgcgggcggt cttgcgagtg ccggtgtcgg tactgtgaaa tttaacggcg gtaccggcgc
    10681 cctgggcacg ggtggcagct ttggcggcgg tggcggctcc tccggcggta cagcggcagc
    10741 aggtgtttat cctgatggca ctaccacagt tcggacaggc ggcacagctc cggcgggcgg
    10801 cggtgatggc ggtgccggtg ctgcttccgg tgtggtcggg caggaaggcg ctgctcccgg
    10861 cggcggcggc ggcggcggtt accgagcggg cagcggtact actcaggcac cgggcaacgg
    10921 cgctcccggc cgtgttgttg taacctatga ctgtgcgatt tcttccgtaa ctttcgatgc
    10981 tgttgctgat cctgatgatg aagtgtatcg ctgcgctgct gccgaaacgg agacctatac
    11041 ggtaactgct cccggtgcca cgactctgac ttattctctt gatgccgcca gccttgccgg
    11101 cggcaatacc attgattctg cgaccggcac ggtgacctgg gatgcggcct gggtgggatt
    11161 cagtactgtc acagtggagg cttcttcttc tggctgcgct cttacggcca acctgcttgt
    11221 gcatacggtt gccctggtgc aggcgcctgt ttttgatgca ggctctaccg tccagagatg
    11281 ccgtgcggcg gaaagcattg tttattctgc gacagctcac catgctgata cgctggttta
    11341 cagtcttgat gctgccagtc ttgctggtgg caacacaatc gatagcgcca ccggtgaagt
    11401 gaccttcgtc gccacctgga gcggcacatc tactgtgaca gcaaccgcta cgggctgcgg
    11461 caccactaaa acggctgatc tgactgtgtt gaccggtgcc ctgtttgcca atgacgatta
    11521 tgtggtcaca ctgcagggca atcttgctca ttttaatgtg ctggacaatg atctttgtaa
    11581 tattgatgcc agttcggtca ctcttatctc ttctcctatc ggcggatttg tccaggtagg
    11641 tgccggtggc gagatgacct atgtcagttt tggcgctttt gagggcttgg atcagttcac
    11701 ctatcaggtc tgtaccccgg cgccggtgca atgcaagcag gcaactgtgt atattcaggt
    11761 gaacccggcg ttggacgatc cctgttacgt tgcgaataag gcaagtcttt tttatctgcc
    11821 ttatccggaa aatgagacgc atcttcgcca gagtctgctg agtgccggga gccaggcgat
    11881 gagcggaggt tcgaacggcg gtaccgatgt aagaacagtg gtgagtattc tggtgcccta
    11941 tcccggcacc cgtattgtat atgaccattg ggaagacggg tatgaggccg acagtaattt
    12001 cccggtgcag tctactacgg aagtctgggg cgatggtgat ctgaccaatg gtgtggcacc
    12061 tggctacccc acggatttca ttcccggtgg cggatatctt attattgaca ataccttccc
    12121 ttggaatcgt tccattgtca cgattgtccc cgacggaaaa gacaagattt cctcctcttc
    12181 ttctattata gtgtcgaaag tctcgggtga tgcggcgcgc tttgccgtac aaaatgtgaa
    12241 gaccaatgtc agtgaggaaa gctatgccgg ccagcacttt gtgctgcctt tcggtgaaaa
    12301 cgttactgac ggcggaacct ccgctttcag atacacagga atgtttgtcc gagcgtatga
    12361 aaacggcacc gtggttgaac tggactataa cggcgacggt acaaacgatg tgacatcacc
    12421 gacgctggat gaaggggacg tctggtttta ccagggcacc gcaagttctc cgggtgtggc
    12481 gggggacgtc aacaactcca acgatattaa agcgggtgcc cgtgtaacgg catcgaaaga
    12541 tgtgagtgtt gatctggtct ttggcggaat tgacaactac ggtacgcgaa atattgccat
    12601 ttatccgagc aactattacg gagataagta ttactctcct gtttattcga ccaatactga
    12661 tgcgctagcc tatgctttct gcgcctcccc ccatttttaa accacgaagc gccaaattta
    12721 aggtggaaac tcca
//
LOCUS       DAOPWB010000089        12701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5038_length_12701_cov_6.118931, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000089 DAOPWB010000000
VERSION     DAOPWB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5038_length_12701_cov_6.118931"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PKN92_08925"
     CDS             <1..952
                     /locus_tag="PKN92_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48955.1"
                     /translation="IRELGAVLFYSHTEKDRMWDLPELEGMEIYNIHTDYLEEPKGII
                     QKMLPQLLLTVKPYPQQSLRIIFDPPAGQLARWDDLNRTRHITGIAANDTHQNVGVRA
                     IYTPNDTVILKGTGEKHKIIKEFKLNFLTRALLKLCCGPLTPDQEFWRFELDAYHVSG
                     HHVNTHVLAKDLTEESILDALRAGRAFVAFTAIADATGFITYIEGDQGKVVMGESIAY
                     QPGMQLVAAAPNQCRFLLLNHGKEIARAEGARCTFDIPGPGKYRIEAHLNILGEWTPW
                     IYANPIEVTETAPEPPALPAPAEEETEAAEADAVATDDAA"
     gene            966..2219
                     /locus_tag="PKN92_08930"
     CDS             966..2219
                     /locus_tag="PKN92_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ48956.1"
                     /translation="MNSKRAFRYLCYISLLLFVSAGLYALGAVLAEQDTDPRETAFPS
                     SEMPDQLCLTWSDSPQHTQTIQWRTSPAVRTGQVQYHPAAEGEENAHTADASLFVLKD
                     SGVINDPVNHRFTAVLRDLIPGTVYRYRVGAASRWSDWSEFVTAPAGTAPFSFVYLGD
                     PQVGFDQWSQLLQSAITRCPDAAFYAVAGDQVNRGTHRNEWDELFRAATGIFDRRPYV
                     PAPGNHDYARRWKPKMYLDLLTLPENGPVCPGPELSYAFHYSNALFVVLDSNRFIRAQ
                     KAWLEEQLKNTKATWKFAIFHHPAYSSKANRDNRTIRKVWGSLFDTYHVDMALQGHDH
                     GYLRTKPMHGGLVAASPAEGTVYVVSVSGAKRYAVGDFSYAEKTLDHTATWQHIDIQT
                     EGGDVLTYRAYTQEGTLVDELTIRK"
     gene            2367..3503
                     /locus_tag="PKN92_08935"
     CDS             2367..3503
                     /locus_tag="PKN92_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNZ48957.1"
                     /translation="MHLFVPKEHDSRETRTALTPDGAAQLIKLGAEVTVEAGLGEKSG
                     FPDALYENAGARLTSDRNAALQAASAVLQVCKPSVADVALMKEGTLLICLLEPFTSFD
                     VLDALEKQKVNAVSLEMIPRTTLAQKMDVLSSQANLAGYVAVLTAANRLSQVLPMMMT
                     PAGTLKPARVFIIGVGVAGLQAIATAKRLGARVEAFDTRPAVEEQVHSLGAKFVKIDL
                     GETGQTKDGYAKELTEEQKELQKAGMAKVIAQSDIVITTAQVFGKSAPRIVSRAMVEA
                     MKPGSVVVDLAAETGGNVEGAQAGAEVNINGVRLIGAENFAGRVAGHASQMFSSNSVN
                     FITHFWDKENKDIHLNLEDEIMDGCLIILNGALRNEMIRKARQA"
     gene            3503..3796
                     /locus_tag="PKN92_08940"
     CDS             3503..3796
                     /locus_tag="PKN92_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNZ48958.1"
                     /translation="MDITLLLFTFVLAIFLGFELISKVPSQLHTPLMSGSNAISGISI
                     VGALLASGLSSDTTLGLILGLVAVICATINVVGGYLVTDRMLSMFKTREGSQK"
     gene            3793..5184
                     /locus_tag="PKN92_08945"
     CDS             3793..5184
                     /locus_tag="PKN92_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNZ48959.1"
                     /translation="MNILIDLSYIVASILFIFGLKMLGKPTTAVRGNQISAIGMLVAI
                     VATLLYSGMDYRWIIVGLVIGSGIGAVAAIRVPMTSMPEFVALFNGTGGLASLLVGWS
                     EYNKFLSTPDGTLEVVPVVAIFLAVLIGGVTFTGSMIAYAKLAERIDGKPTLFAGQQA
                     VNALIVAALVISGAFYIMNPGAGLSQILFYITVVLSLVLGVLVTIPIGGADMPVVISL
                     LNSYSGLAGCAAGFVISNTVLIVAGSLVGASGIILTSIMCKAMNRSLAHVLFSGFGAA
                     SSTGGGSTETGEVKPISAEDAYYVLEAASSVVFVPGYGMAVAQAQHAVRELGDLLEAN
                     GAEVKYAVHPVAGRMPGHMNVLLAEANVPYEQLVEMDDINPIISSVDVCVVIGANDVV
                     NPGAREDESSAIYGMPIINVDLARTCFVLKRSMRAGFAGIENPLFFKENTRMLFGDAK
                     ESITTLVEQFKSS"
     gene            complement(5230..6909)
                     /gene="thiC"
                     /locus_tag="PKN92_08950"
                     /pseudo
     CDS             complement(5230..6909)
                     /gene="thiC"
                     /locus_tag="PKN92_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183113.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            complement(6982..8481)
                     /locus_tag="PKN92_08955"
     CDS             complement(6982..8481)
                     /locus_tag="PKN92_08955"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNZ48960.1"
                     /translation="MTSANSLDQLKARLRDVHHLRAAEALLSWDQEVYMPPKGAAARG
                     AQIAALSAQAHTLFTAPETGALFDSCLSEQDRCTPDDRALLQEAFYEYNRAVRIPESL
                     VREFAEAQSAAYHAWLEARKQDNFPLFLPHLEKLIALSKKQAACLNPELPPYDALLEN
                     YERGISVDEVDSIFSLLEKEQTPLLQEITEMKASAPPPVIPEGWEIEAQRRFTEQILA
                     DMGFDFEAGRQDVSAHPFTTNFSIGDVRVTTRYSEKEPFSALFSSIHEGGHALYEQGF
                     QEKDEGTLLAAAPSLGIHESQSRLWENMIGRSRAFWSHYGAVFRAHFPDRGGHLSDED
                     LYRAVNRVSPSLIRVDADECSYNLHVIIRFQIEKALFNGDLEAKDVPAVWREKMTDFL
                     GVTVPDDRQGCLQDIHWSHGAFGYFPTYALGNLYAACILEKAESEIEGFYEKIASGQF
                     QPLLHWLRTHVHEKGRRALTLPLLTEVCGKTPEPDSFLRYLKNKYLAAD"
     gene            complement(8617..10572)
                     /gene="htpG"
                     /locus_tag="PKN92_08960"
     CDS             complement(8617..10572)
                     /gene="htpG"
                     /locus_tag="PKN92_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNZ48961.1"
                     /translation="MSQSETFTFKTEARQILELMVHSVYSNKEIFLRELISNSSDALD
                     KRRYESISNPALESASELEIRIEADPAARTLSIIDNGIGMSRDEVHQFIGTIAKSGAR
                     EFLNALKEKKEGDSSPDLIGQFGVGFYSSFMVADKVSLITRRAGTDEATLWESSGQGE
                     YTIGDATREEAGTTVTLYLKDPDQEDSLQDYTDSYEIRRIVKKYSDFVSYPIRLTVVK
                     AEEDLAEDADADSDTDTAAASDEPLNSMKAIWLRSKEEVTDEEYNEFYKHLTHDWNNP
                     LFRIQAKIEGTLEYRMLLYIPERMPYDLLYSQQALQHGLHLYIKRIFIMDNCRDLLPS
                     YFRFVRGVVDSEDLPLNISRELLQENRQVQRMNKGIVAKLLQELQSLQETDREKYRAF
                     WTQYGAILKEGITMDPDNRAALLNLSLFQTTRSGDEPASLKEYVERMQEGQKHIYYLS
                     GESLAALKDSPHLEAFRSKNIEVLLLSDGSDEFWTHHADKYGDYEFKSAARGALDLDE
                     VQQEDKEEEKPEAKEDRFRGLFAVLSEILSDHVKEVRASARLVDSAACLVIDEADLSP
                     QLEKMMKLMGQQMPKTKRILEINTNHAVAAGLLKHFEKDPSKDANTLFAETLYGMALL
                     AEGGEVDNPAALSKNIGMLLAQALDQS"
     gene            complement(10608..11909)
                     /locus_tag="PKN92_08965"
     CDS             complement(10608..11909)
                     /locus_tag="PKN92_08965"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNZ48962.1"
                     /translation="MRLSIVHEGSGNLKYEIREIVSVAREIRSLGQNITWENIGDPIE
                     KGEVIVPWIKEILRDLLEQPESFGYCDTAGIPNTRAFLADTVNARPDGVKITPEDIIF
                     FNGIGDAVARIYGFLKREARVLGPSPAYSTHSSAEAAHSGYGPVTYDLDPQSNWMPNI
                     EDIRNKVRYNDSIAGILLLSPDNPTGAVYPREVLDQIVAIAKEHDLFMIADEIYTHIT
                     YPGFPQMHMSQWIQDVPAIAMRGISKEYPWPGARCGWIEILNRDKDANFSQYVDSLLA
                     AKRLEVCSTTLPQMSIPRVYSDPRYKGHLANRALMFSKRADEAAAAFEGCDSVIVNKP
                     GGAFYMTVMFKEKLLNNKQTLPIENPKVKERIEALVKNVSNDKRFVYYLMGSTGIVVV
                     PLTGFQCSHPGFRVTLLESNEEKRRWIFRTLREAIDAYIAS"
BASE COUNT         2869 a         3381 c         3430 g         3021 t
ORIGIN      
        1 cattcgtgaa ctgggcgccg tgctttttta ctcacacacc gagaaagacc gtatgtggga
       61 tcttcctgaa ctggaaggca tggagatcta caacattcac accgactatc tggaagagcc
      121 taaaggaatc atccagaaga tgctgccaca gctgctgctc acggtcaagc cctatccgca
      181 gcagtctctg cgtattattt ttgatccccc tgcaggtcag cttgcccggt gggatgatct
      241 gaaccgtacc cgccacatca caggtattgc cgccaatgac acccatcaga atgtgggtgt
      301 acgcgccatc tatacaccca atgacaccgt tattcttaaa ggaacgggcg agaaacacaa
      361 gatcataaaa gaattcaaac tcaattttct gacccgtgct ctgctgaaac tctgctgcgg
      421 accgctgaca cccgatcagg aattctggcg ctttgaactg gacgcctatc acgtctccgg
      481 ccaccatgtg aacacccatg tgctggcaaa agacctgaca gaagaaagca ttcttgatgc
      541 attgcgcgcc gggcgcgcct ttgtcgcatt taccgccatt gccgatgcaa caggttttat
      601 tacctatata gaaggcgatc agggcaaagt ggtcatgggc gaaagcatcg cctaccaacc
      661 cggtatgcag ctggtggcgg cggccccgaa ccagtgtcgc ttccttctcc tgaatcatgg
      721 aaaagaaatc gcccgcgccg aaggtgcccg ttgcactttt gatatccccg gccccggcaa
      781 ataccgtatt gaagcgcacc tgaacattct gggtgaatgg acgccgtgga tttatgcaaa
      841 tcccatcgag gtaaccgaaa cagctccgga acccccagcc ctgcccgcac ctgccgagga
      901 agaaacggaa gctgctgaag ccgacgccgt tgccactgac gatgcagcat gaaaggccgt
      961 aaccattgaa cagtaaacgg gcgttccgtt atctctgtta tatcagcctg ctgctgttcg
     1021 tctcagcggg attgtatgcg ctgggcgccg tgctggcgga acaggacaca gatccccgcg
     1081 aaacggcttt tccttcatca gaaatgcctg atcagctgtg cctgacctgg agcgactctc
     1141 cgcagcacac acagacgatt cagtggcgta cgtctcctgc tgtacgcacc gggcaggtgc
     1201 agtatcaccc ggcggcagaa ggcgaagaaa acgcacacac cgccgatgcc tccctgttcg
     1261 tgctgaaaga cagcggcgta atcaatgatc cggtgaatca tcgcttcact gccgtgcttc
     1321 gcgatctcat cccgggcacg gtgtaccgct accgggtcgg tgcggcatcc cgttggagcg
     1381 actggtcgga attcgtcact gcccctgcag gcacggctcc cttctctttt gtttatctgg
     1441 gcgatccaca ggtgggcttt gaccagtggt cacagctgct gcagagtgcc attacccgct
     1501 gccccgatgc cgctttttat gcggtagcag gtgatcaggt caaccggggc actcatcgta
     1561 atgaatggga cgaacttttc cgtgcggcga caggaatatt cgacagacgc ccttatgtgc
     1621 cggcgccggg caatcatgac tacgcacgac ggtggaagcc gaagatgtat ctggatctgc
     1681 tgacgcttcc cgaaaatggc cctgtctgcc ccggtcctga attatcctat gcctttcact
     1741 acagcaacgc tctttttgtc gtactcgatt cgaatcgctt catccgggca cagaaagcct
     1801 ggctggaaga gcagctaaaa aatacgaagg caacctggaa atttgcaatt ttccatcatc
     1861 ccgcctattc gagcaaagcc aacagagaca accgcacgat acggaaagtc tggggcagtc
     1921 ttttcgacac ctaccatgtg gatatggcgc tgcaggggca tgaccacgga tacctgcgca
     1981 caaaacccat gcatggcggg ctggtggcag cctcgcctgc agaaggcacc gtctatgtcg
     2041 tatctgtctc cggggcaaaa cgctatgccg tcggtgattt ttcctatgcg gaaaaaacgc
     2101 ttgatcacac tgccacctgg cagcatatcg atattcagac ggaaggcggt gatgtgctga
     2161 cctaccgcgc ctatacgcag gaaggaactc ttgtggacga gctgaccatc agaaaataag
     2221 agcaaatacg gcacattcac ccatttttca gttttttaat ccccggaagc acaaaagcgg
     2281 gcttccgggg attaattttt taactgcata tgttaaaaac taccaagatc cccaatgccg
     2341 caaggcgaca ggaagaggag cctctcatgc atctatttgt cccaaaggaa cacgacagcc
     2401 gggaaacacg aaccgcgctg accccggatg gtgccgcaca actgatcaaa ctgggtgcgg
     2461 aggtcaccgt cgaagcaggg ctcggtgaaa aaagcggatt cccagatgct ctttacgaaa
     2521 acgccggtgc acggctgact tccgaccgca acgccgcact gcaggcagca tctgctgtgc
     2581 ttcaggtctg caagccctcc gtggcagatg tcgccctcat gaaagaaggc acgctgctta
     2641 tctgcctgct cgaacccttc acttcttttg acgtgctgga tgcgctggaa aaacaaaaag
     2701 taaatgctgt ctctttggaa atgattccga ggacaacgct ggcgcagaag atggacgtgc
     2761 tcagctccca ggcgaatctg gcgggctatg tggcggtact gacagctgca aaccggctca
     2821 gccaggtact tcccatgatg atgacaccgg caggcacact gaaacccgca cgggtcttta
     2881 tcatcggtgt gggcgtggca gggcttcagg caatcgccac agcgaagcga ctgggcgcgc
     2941 gggtcgaggc ctttgataca cgtccggcag tggaagaaca ggtgcactcc ctcggcgcaa
     3001 aattcgtgaa gatcgatctc ggcgaaacag gccagaccaa agacgggtac gcaaaggagc
     3061 tgacggaaga acagaaggaa ctgcagaaag cgggcatggc gaaagtgatc gcccagtccg
     3121 atattgtcat cacaacggca caggtcttcg gaaaatcagc cccacgaatt gtatcccgcg
     3181 ccatggtgga agccatgaaa ccgggcagtg tcgtggttga tctggcggcg gaaacaggcg
     3241 gcaatgtgga aggtgcgcag gcaggcgccg aagtgaatat taacggggtg cgcctgatcg
     3301 gtgcggaaaa cttcgccggt cgggttgcag gacacgcttc ccagatgttt tcctccaaca
     3361 gcgtgaattt cattacccac ttctgggaca aagagaacaa ggacattcat ctgaatcttg
     3421 aggatgaaat catggacggc tgccttatta ttctgaacgg cgccctccgc aacgaaatga
     3481 tcaggaaagc gaggcaggca taatggatat cactcttctt ttattcacat ttgtgctggc
     3541 gatttttctg ggcttcgagc tcatcagcaa agtgccttcc cagctgcata cgccgctgat
     3601 gagcggttcc aatgccatct cgggcataag tattgtcggt gcgctgctgg cgtcgggact
     3661 ctcctccgat accacactgg gtctgatcct gggactggtg gcggttatct gtgccaccat
     3721 caacgtggta ggcggctatc ttgtaaccga ccggatgctt tccatgttta aaacccggga
     3781 aggaagccaa aagtgaatat cctgattgat ctctcctata ttgtcgcatc catcctattt
     3841 attttcgggc tgaaaatgct gggcaaaccc accactgccg tgcggggcaa tcaaatatcc
     3901 gctatcggca tgctggtggc gattgttgcc actctgctct acagcggcat ggactaccgt
     3961 tggatcattg tggggcttgt catcggcagc ggcatcggtg ctgttgccgc catccgcgtg
     4021 cccatgacct ccatgcccga attcgtcgct ctctttaacg ggacaggcgg ccttgccagc
     4081 ctgctcgtag gctggtctga atacaacaaa tttctcagca caccggacgg aacgctggaa
     4141 gtcgtgcctg tagtcgccat ctttctggcg gtgctcatcg gcggggtcac cttcacaggc
     4201 agcatgatcg cctatgcgaa actggcggaa cgcatcgacg gcaagccaac tcttttcgcc
     4261 ggacagcagg cggttaatgc actcattgtg gcggcgctgg ttatcagcgg cgccttctac
     4321 atcatgaacc ccggtgcagg tctgtcccag attctgtttt atattacggt cgtcctttct
     4381 ctggttctgg gcgtgcttgt aaccatcccc atcggcggtg ccgatatgcc cgtagttatc
     4441 tctctgctca acagttattc cgggctggca ggctgcgctg ccggttttgt catctccaat
     4501 actgtgctga tcgttgccgg ctctctggtc ggtgcaagcg gcattatcct gaccagcatc
     4561 atgtgcaaag ccatgaaccg ctctctggca catgtacttt tcagcggttt cggcgctgca
     4621 tcttcgacgg gcggcggaag cactgaaaca ggcgaagtga agcccatctc cgcagaagat
     4681 gcttattatg tccttgaagc ggcgagctcc gtcgttttcg tacccggtta cggaatggct
     4741 gtcgcccagg cgcagcatgc ggtacgtgag ctgggcgatc tgctcgaagc caacggcgcc
     4801 gaagtgaagt atgccgtgca ccccgtggct gggcgcatgc ccggccatat gaatgtgctg
     4861 ctcgccgagg cgaatgtgcc ctatgagcag ctggtggaaa tggacgatat caaccccatt
     4921 atcagcagtg tggacgtgtg cgtggtgatc ggtgccaatg acgtggtcaa tcccggtgca
     4981 cgggaagacg agtccagcgc catctacggc atgcctatta ttaacgtcga tcttgcccgc
     5041 acctgctttg tgctgaagcg ttccatgcgt gcaggttttg cagggattga aaatcctctt
     5101 ttcttcaaag aaaatacacg catgctcttc ggtgatgcga aagaaagtat cactactctg
     5161 gtggaacagt ttaaaagcag ctgagcaggc gaggaaacgt cacgaaggtc tgttgtgaac
     5221 ggcgcagctc tgccggaaaa ggcgcatgga acaaaatttc gggccgcaca tgctgcagaa
     5281 tccgtcttcc agagaggact tgctttccgg aaaggactgc aaccggatct gcaaggcatg
     5341 atcgggatca agggaaaggg caatctgatc tgcccagcgg aattccatcc gcgccttgct
     5401 tagggcattg tccctgatct gtgcacccgg cagtccttta gcaagatcag cggcgtgtgc
     5461 ggcaagctta tgagtcacga caccacggcg cacatcgtct ctgtcgggca gtcccagatg
     5521 ctcccgcggt gtgacatagc agagcatgga cgcaccttcg gcggcaatca tggcgccgcc
     5581 cagagccgag gtgatgtggt catagccggg tgcgacgtcc gtgggcagcg gaccgagggt
     5641 ataaaaaggc gcttcgtgac accattcggc ctgaaggcgc ctgttttccg caataagatg
     5701 catgggcacg tggccgggtc cctcattaat cacctgcaca tactgatccc aggcacggag
     5761 cgtaagctct ccctgggttt tcagttctgc aagctgcgcc tcatcgttgg catcataaat
     5821 actgcccgga cgtaacccgt cgccgatgga tacggcaatg tcataggccg caaggatttc
     5881 acaaacatca tcccaatggg tataaaggaa gttttctttc tggtgatagc ggcaccagtg
     5941 cgccataatc gcaccgccac ggctgacgat tcctgtcatc cgtttttcgg tcagaggaaa
     6001 atgcgcctgc agtgcggcgg catgaattgt gaaataatcc accccctgtt ccgcctgctc
     6061 gatgagtact tctttaaaga gagaccaggt gagtgcttcc ggctttcctt cggcacgttc
     6121 cagcgcctga tagagcggca cagttccaat aggcaccggg gcattgcgca gtatccattc
     6181 ccgtgtatca tgaatatcgt cacccgtact caggtccatg actgtatcgg caccccacag
     6241 cactgcccag accattttct ccacttcttc cgaaatgctg ctgtgcagcg ctgaattgcc
     6301 gatattcgca ttgattttga cggcgaagcg gttgccgatc agcatgggtt ccgcttcggg
     6361 gtgattgata ttggcaggca ggatggctct gcccgatgca atttcttcac gcaccagttc
     6421 cggagtgatg gcgctgtact gccggggcgg gaatgttctg tgtgccagtg tgtcactgta
     6481 ccgctccatc agcacatttt cccggatggc ggcatattcc atctcttcgg tgatgatgcc
     6541 ctgctgcgcc agatagcgct gcgtaatatg ttttccggaa cgggcacgac gcactgttct
     6601 tttgcctgga aaggctgtgg aacaggcgtg cggtgcctct tctcttgtct caagatcggt
     6661 gcgggcatcc agccagcctt tccgcagagg tggcagccct ttttcgtagg ccactgtaaa
     6721 atccggatcg gtgtacgggc cggaggtgtc ataaagaacg acaggcggat tggtatccac
     6781 caattctccg gtttcgctga ccgtgggatg ctgtgacact tcgcgcatgc ctacggaaat
     6841 gccgggccgg cttcccttta tataaatctt gcgggattga atgaattcag gaaaggagag
     6901 tcccttcatg agagaggaag tttcattatt ctgtttttcg ggcatgggct ctgctttctt
     6961 tcacacatcc ggttcagcgg atcagtccgc cgccagatat ttattcttca gataccggag
     7021 aaaactgtcc ggttccggtg ttttaccgca tacttcagtg agaagcggca gggttaatgc
     7081 tcttcgtcct ttttcatgaa catgtgttcg gagccagtga agcagcggct gaaactgtcc
     7141 ggacgcaatc ttttcataga agccttcaat ttcgctttcc gctttttcca gaatgcaggc
     7201 ggcatagagg ttaccgaggg cataagtggg gaaataaccg aaagcgccgt gggaccagtg
     7261 gatatcctga aggcatccct gtcggtcatc gggaactgtg actccgagga aatctgtcat
     7321 tttttcacgc cacaccgccg gaacatcctt tgcttccaga tcgccgttga agagtgcctt
     7381 ttctatttga aagcggatga tcacatgcag gttgtaactg cattcgtctg catcaacccg
     7441 gatcagtgac ggcgagaccc ggttgaccgc acgatagaga tcctcatcgg aaagatgccc
     7501 gcctctgtcg ggaaaatgcg cgcgaaagac agccccgtaa tgactccaga aagcgcgact
     7561 tctgccaatc atgttttccc agagccggga ctgggattca tgaatgccga gagaaggagc
     7621 ggcagccaga agcgtccctt catctttttc ctgaaagccc tgctcataaa gggcgtgacc
     7681 gccttcgtga atggaactga agagagcgga gaagggttct ttttccgagt agcgggtggt
     7741 aacacgcaca tcgccaatgg aaaaattggt tgtgaagggg tgagcggaaa catcctgcct
     7801 gcctgcttca aaatcaaaac ccatatcggc gagtatctgc tcggtgaaac gccgctgtgc
     7861 ttctatctcc cagccttccg ggattaccgg cggaggggca gatgccttca tttctgtaat
     7921 ttcctgaaga agcggcgtct gctctttttc aagtaatgaa aagatgctgt ccacttcatc
     7981 gacagaaatg ccccgttcat aattttccag cagtgcatca taaggaggca gctccggatt
     8041 gaggcacgcc gcctgttttt ttgaaagcgc aatgagtttt tccagatgag gcaggaaaag
     8101 agggaaattg tcctgtttac gggcttcgag ccaggcatga taagcggcac tttgtgcctc
     8161 ggcaaattcc cgaaccaggc tttcaggaat acgcaccgcc cggttgtatt cgtaaaaagc
     8221 ttcctgaagc agtgcccggt cgtcaggcgt gcagcgatcc tgttccgaca ggcaggaatc
     8281 aaagagggca cccgtttcag gggcagtaaa gagcgtatgc gcctgggcac tcaatgcggc
     8341 gatctgggca ccccgcgccg ccgcgccttt aggcggcata tacacctcct gatcccagga
     8401 gagaagcgct tccgcggctc tgagatgatg aacatcccgc aatcttgctt ttaattggtc
     8461 taaagaattg gcggaagtca tgatgctctc catttctctt gccggcgctg ctgctgtgtc
     8521 ttgaatcaaa tttatatact accgcaacga cacatgacac accaaagacc gtcttttttt
     8581 tcaggcggct tcgaaactgc tgtattgcct gactcctcag gactgatcca gcgcctgtgc
     8641 cagcagcatg ccgatatttt tgctcagtgc ggcggggtta tccacttccc cgccttccgc
     8701 aagaagcgcc atgccgtata gcgtttcggc aaagagtgtg ttggcatctt tggacgggtc
     8761 tttttcaaaa tgcttgagca gtccggcggc aacagcatgg ttcgtattga tttcgaggat
     8821 acgcttggtt ttcggcatct gctgtcccat gagcttcatc attttttcta attgcggact
     8881 gaggtcagct tcatcgatga cgagacaagc cgctgagtct acaagacggg cagacgcgcg
     8941 cacttctttc acatgatcgg agaggatttc ggagagcact gcaaaaagac cccggaagcg
     9001 atcttcttta gcctcaggtt tttcctcttc cttgtcttcc tgctgcacct catccagatc
     9061 cagggcgcca cgggcggctg atttaaattc gtaatcacca tatttgtcgg cgtggtgtgt
     9121 ccagaattcg tcggagccat cgctgagcaa cagcacctcg atatttttgg agcggaacgc
     9181 ctcgagatga ggcgagtctt tcagtgctgc cagagattca ccggacaggt aataaatgtg
     9241 tttttgccct tcctgcatgc gctcaacata ttctttcaga gaagcgggct cgtcaccgga
     9301 acgggttgtc tggaacagag acagattgag cagcgccgct ctgttgtccg gatccatggt
     9361 aatcccttct ttcagaatgg cgccgtactg cgtccagaaa gcccggtatt tttcccggtc
     9421 agtctcctgc agggactgca gctcctgcag cagctttgca acaatccctt tgttcatgcg
     9481 ctgcacctgc cggttttcct gcagcagctc acgcgaaata ttgaggggca ggtcttcgga
     9541 gtccactaca ccccggacga aacggaaata ggaaggaagc agatcccggc agttgtccat
     9601 gatgaaaatg cgcttaatat acagatgcag accgtgttgc agcgcctgct gtgaatagag
     9661 caggtcgtag ggcatccgct cggggatata aagcagcatt ctgtattcca gcgtgccttc
     9721 tattttcgcc tgaatacgga acaggggatt gttccagtcg tgtgtgagat gcttgtaaaa
     9781 ctcgttatat tcttcgtcgg ttacttcctc ttttgagcgc agccagatgg ctttcatgga
     9841 attgagaggt tcgtccgaag ctgctgctgt atcggtgtcg ctatcggcgt ctgcatcttc
     9901 tgccaggtct tcttcggctt tgactactgt caggcgaatg ggataggaaa cgaaatcact
     9961 gtatttcttt acaatccgcc ggatttcata ggaatcggta taatcctgaa gactgtcttc
    10021 ctgatcggga tctttcagat aaagcgtgac cgtggtgccc gcctcttctc ttgtcgcgtc
    10081 gccgatggtg tattcaccct ggccgctgct ttcccagagt gtggcttcgt ccgtgcctgc
    10141 ccgacgggtg atcaggctaa ctttgtctgc taccataaaa gaagaataaa agccgacacc
    10201 gaactgcccg atcagatcag gagagctgtc gccttctttt ttttctttca gcgcgttcag
    10261 aaattcgcgg gccccggatt tggcgatggt accgatgaac tgatgcactt cgtcccggct
    10321 catgccgatg ccgttgtcga tgatggaaag cgtccttgct gccggatcgg cttcaattct
    10381 gatttccagc tccgatgcgc tttccagagc aggattgctg atgctttcat aacggcgctt
    10441 gtcgagggca tcggagctgt tggaaatgag ctcccgcaga aaaatctctt tattggagta
    10501 gacggaatgc accatcagct ccagaatctg gcgtgcttct gttttaaacg taaaagtttc
    10561 agattgactc acggcttaac tcctcttcgc aatacacaaa actgcaatca actggcgata
    10621 taggcatcaa tcgcttcgcg cagcgtccgg aaaatccagc ggcgcttttc ttcattgctc
    10681 tccagcagcg ttacccggaa accggggtga gagcactgga agccggtaag aggcacaacg
    10741 acgatgccgg tagaacccat gaggtaatac acaaaacgtt tatcattgct gacatttttt
    10801 acaagcgctt caatacgttc cttcactttc ggattttcaa tgggaagggt ctgtttattg
    10861 ttgagcagtt tttccttgaa cattacagtc atgtaaaagg cgccgccggg cttattgaca
    10921 atgacggagt cacagccttc aaaagcggcg gctgcttcgt cggcccgctt gctgaacatg
    10981 agcgcacggt tggcaagatg gcctttgtat cggggatcac tgtagacccg ggggatggac
    11041 atctgcggca gggtggtgga gcacacttcc agacgtttgg cagcgaggag gctgtccacg
    11101 tactgagaga agttggcgtc tttgtcgcgg ttcagaattt caatccagcc acagcgtgcg
    11161 cccggccagg ggtattcctt gctgataccg cgcatggcaa tggccggtac atcctgaatc
    11221 cactggctca tatgcatctg cgggaacccc ggataggtga tgtgggtgta aatttcgtcg
    11281 gcaatcatga agagatcgtg ttctttggcg atagcaacaa tctgatccag cacctcccgg
    11341 ggatacacag cgcctgtagg gttgtccggg ctcagcagca gaataccggc gatggaatcg
    11401 ttgtaccgca ccttgttgcg gatgtcttca atattgggca tccagttgct ctgcggatcc
    11461 agatcatacg tgaccgggcc gtagccggaa tgcgccgctt ctgctgagga atgggtgctg
    11521 tatgcaggag acgggccaag tacccgggcc tcccgtttca gaaaaccgta aatgcgggcg
    11581 acggcgtcac caatcccgtt aaagaaaatg atatcttccg gcgtaatttt gacaccgtcc
    11641 ggccgggcat tcaccgtatc ggcaagaaaa gcacgggtgt tggggatccc tgccgtatcg
    11701 caatagccaa aggactcggg ctgctcgaga agatcccgga ggatttcttt gatccaggga
    11761 acgattactt cgcctttttc aataggatca ccgatatttt cccaggtgat attctgtccc
    11821 agagagcgga tttctctggc tacgcttacg atttcacgga tttcatattt aaggttgccg
    11881 gagccttcgt gcacaatgct gaggcgcatg agcgtttttc cttgatagtt ttcgcttgtt
    11941 aaaaaaggtt tgcagccgct caaaagaggg cagcgagacg aattctctga cggaaagcct
    12001 gcaaaacacc tgccaaaata gtagcccttt tggcacagga aatgcaaaaa gatcggaaag
    12061 gcgaaaaacg atttgacagc ggaacctgca tctgaggaga tgctctctat ttcggcggca
    12121 gcagacaata aagatcgaat ggccggagaa ggcaggatcc cttcttttga cttttatgcc
    12181 gaaatcccgg cattcaggaa aatgccgcct ctgcactaaa attctctggt gccttcccgg
    12241 gtaagcagta tggggcggta ctttcgtgct aatagttgat tcgggcaata gcgacaactg
    12301 aagagcagtc ccagacgttg cccttatgga gaagaaatgc ggttctccgg caggatctga
    12361 ttttggcttt ttttcttctg atcctgtgtt tctttgggcc ccggggtgag gcaacggcat
    12421 gaaatgccac cgccaaagaa ggaattggca ctcgtaggct tttgtttcag cctggtatga
    12481 atgttacact atttaacagt aatctatttc ccccttgtgt tctatgcagg taattaacca
    12541 taaaagaaaa acaggtataa ggagtactcc ggatgaacag caagacccgt agcaatgcgt
    12601 ttttgcactg catcacagca gtgggcgctc tgctgctggc gctggcaatg cctgccgcac
    12661 tggcacagca gaatttgatc gtgaacggat cctttgaagc g
//
LOCUS       DAOPWB010000090        12558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5105_length_12558_cov_5.005359, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000090 DAOPWB010000000
VERSION     DAOPWB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12558
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5105_length_12558_cov_5.005359"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1609
                     /locus_tag="PKN92_08970"
     CDS             131..1609
                     /locus_tag="PKN92_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNZ48963.1"
                     /translation="MASGRKEKQVAVPKEKTARKDEKKTSAKASSGAKEAPVKKKATA
                     VKSHPEKKAAKVPKAGAETPGKKADAEKKVKKVIQAKPSAVKAADKPATAKKPAAAVK
                     PAPEKKASRTKTPAVAAAQTPKEDAAPDKAVAKSSPAAKKTPPKAAGKTTSAEAVEVS
                     EAPVTSDKARFTTVNTQFLVSVAQAIKNAVLPMIQSLKGREIVSDAPSGDVTFQIDRV
                     AEKALNAYLQSANLPLSLYSEDVGYSTITSKPPKHLLIVDPIDGTRAAKNGFEGCVVA
                     LASTRVLERPTIGDIECACVMELTADRYFVAERGKGAKYYVNGSLKRPKLSANTDLEA
                     LTWSMTVPARPAELIFPTAARLIDLSSLKGGFFSCNSTSFSLTRLLTNQMDACVDFAN
                     RYYRDMPEELRKVFHFAGKGKVIGIAPYDMAAALLIAQESGCIVTDAYGQSFDDLLLT
                     DTTEGNHRSLIAAANKALHDKLLTFFEARIQQYQEKLKLLHN"
     gene            1693..2244
                     /locus_tag="PKN92_08975"
     CDS             1693..2244
                     /locus_tag="PKN92_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNZ48964.1"
                     /translation="MSVVLVPLAPGCEELEAVTVIDIMRRGGLQVISAGLNQTVVSAS
                     RGVVLQADQLLEEAILNEEVSVVVLPGGMGGTRALMNAPFFTGWLKKRALEGMPVAAI
                     CAAPLILDQERLLSGKRYTAYPGVLDVALSENACYTGTVLEADGNIITSRGPGTAMEF
                     ALYLVELLAGKARRAEVEAGLVR"
     gene            2415..6446
                     /locus_tag="PKN92_08980"
     CDS             2415..6446
                     /locus_tag="PKN92_08980"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HNZ48965.1"
                     /translation="MKNRRTVAGMIAGLFLMALILPGCPPQVGVELVLSKDTLDFGHD
                     RTEDGLAVHVNHTGTRTEPLEVSADQPWIIPQNCLDFKDGCISQGPYHDLQIRVRIDR
                     DKLAMGLNTGTLRLHANPASPKTVEVVAEEIAQADFYVRQSQVALGKAVEFIDASQVS
                     EKAGRLVSWKWEFGDGNESTAINPVHVYERPGVYDVRLTIQTSENIKRTVLQSALIRV
                     ENPAAHVDFDVSTKAPGLGEEVIFTDRSSLDNITVTGRKWIFGDGTETNFSKEKTAVH
                     RYAKTGVFTVTLQLKTSHGLLESVKENFVIVRSGSGPVADFSFESDAGALYVKEEIRF
                     YDRSHPGTGTITDRSWNFGDGTISNEASPVHRYNGKGEFKVTLSVQTEKGYSSKEKTL
                     TLTYRAPKANFTAAPTKQKVDAPIRFTDRSVPGHDGNVAWAWDFGDGNTSTVQHPTHV
                     YTKKGLYTVTLTVTGGDPDGLTDTFTREGYISIVDTIDTGEGEDEEDNGLLDLEHYVA
                     LNDGAYTYSTPRVEPLRVDGRTAGTVYLIDKMTSQRWNPDNSVHPDYVEWFHPMTIFE
                     PAVKLTKTAMLFIDGGSRTSPAKVEPAFARMSVLSGTTIVHIRNIPCQPIVFLDEVIP
                     AGRQDNYSGEDIILRRRTEDDIIAYSYDEYLKAYRETEGKPNFQWPALFPMVKSAVKG
                     MDTAEDVLAKNGITLDGFVVAGASKRGWTTWLTGAADSRIKAIAPIVINVLNMDEHLA
                     HHRKSYGYWAPAIYPYAQEGVFDQLLPNEQDGTISKEAQSLLNRIDPYEYALKGSYPM
                     PKFMMNGTGDQFFVPDSTQFYFNDLKGDKHLCYIPNAGHDLGGIDGVDISDPENPLGK
                     FFAWYLSVTQREALPGFTESFEQDGAIVVRVSPQRLPVRVRLCQATSDKRDFRNEVMK
                     EQGVEWTYTTLSPTSGSTVYRALPPAPKAGHYTAFFIQLEYANTSTLAIPGIPVPNLV
                     FTTGVRTLPIVNGEIVYPEFTGYLANTERPDAVPFSETKLPVAVVYGTPYEMGRYYGE
                     VLGDQINDAVPAIIAASGLSQEVLLDIWEELEGNVLDNRLQEEIRGIARARKVTVTKD
                     MLNMAHAAIIHASSASGIYSTAAYGNLIKDQAPRIHSMHSATVNESMGLKIAPYQCAV
                     MYIPDQGIPHTVFTYAGLTFGRTGINLGGISGVDIPNLTSDADLSTGMPLLREALYDS
                     LNLREAVATARTGLSRGTALVFSDGRNEYRSAYVPAAPGITRFDMTLDQMRYPVPGLV
                     FGTSQDPYETLSFLLNSTISTFTSEVLLNVTNNNALTDSKRNTLNVVYEGEPLTVVIQ
                     TASSSLSARWDIPEYMNMQLLLP"
     gene            complement(6595..7317)
                     /locus_tag="PKN92_08985"
     CDS             complement(6595..7317)
                     /locus_tag="PKN92_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNZ48966.1"
                     /translation="MEYFLTDTGFHIEAENWLEVLFKLLASLFLASVIGIERQRKGRS
                     AGLRTHVLVALGSTLLMLVSAYMYETFGAAAQPFDQARIAAGIMTGIGFLGAGTIITS
                     GHDKLGLTTAATVWFTAALGIAVGAGYLITACLATAFVLAVVIGFAALSPLLPQYGSF
                     LLSMQLPASEGDISRILRHIEEAGRFEVHTTAIQSLEEGHRLQYTFHIHSRSDKDFFR
                     LTEMLHRHYSQAANVSLERIQL"
     gene            complement(7347..9503)
                     /locus_tag="PKN92_08990"
     CDS             complement(7347..9503)
                     /locus_tag="PKN92_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48967.1"
                     /translation="MISSNTQEKPVRTAALAAEPRNLYLISLLLVGVITFFFYFSMTG
                     VPLQGGDLELLVLPRYLHKPISSAALQQELPNAPAARAVLTFLWSLSDGSAKNLRLFC
                     LVLHVLSALYFFGIVRRWTGKPTSVIPPLLGGFLFCVLPTVPPLLAGASGLSQLLGTF
                     FALICANSYLAVTEDPDRRRPLHLFATLAFALLAAGSYYGLIALPLVLLLLDATRLEK
                     HRETRMSPDWIMLFSVTGVLAALGIVVCFSDSFSFRRLYIPYHLLFMVCILFLIRTLL
                     LFRSKILKNILLVLLNLLLIASAVISFQQTMAFWEPLSRIEKTDAPDKIMRGALQCIA
                     LAEKEDIPAERIDQLRQAATLWKTSPPDLSGEGIYKLAFARVLEETNAPDEALAHYAE
                     LREHFSFTENGLEGAAGMARLQEPGENASFILELLESISRHRDLSQAEELQSALTHMR
                     LGDARNAALHFSRLSGLHPQSPEGQLREQSLKIRSTIDALAEESQKKIEADAKSMEGF
                     VSLAESHLLAGNHLQAFYWLEFALRRDSSVPKAWELLGVLSAMKGAEKNFVEKWGALK
                     AADDKAWIDLAHKSAFSQAWDAAFVYLNQPGKSPDFSAEELMAVFSLEQKRLDQAESW
                     FLKAAELMPDSFRPRLFLADLALVRQDKEKAEEWLKAARDLQAPQAELDRRETVLKTG
                     LPAPNAVPDSGVPVPALPASPNTGASPGVPMRTLIE"
     gene            complement(9717..12239)
                     /locus_tag="PKN92_08995"
     CDS             complement(9717..12239)
                     /locus_tag="PKN92_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNZ48968.1"
                     /translation="MPSEIKTGKLSALLILFLLLGTGFLSWWMAQAPAPLNAQAPKED
                     FSAERALEHIKIMAKEPHPASSHANDRVFEYLLDQAAALGLEIEVIDTPFIHEGRNSA
                     EWGRAILGRIRGTDSTKAFAMDAHFDSVPYGSGAADDLSGIATMLETARALQARAPLK
                     NDLIFVFADQEEVGANGAKAFRRHPWFNDVGVMLGLETRGVCGPALMFETSQNNGKLI
                     RELKKSGVSARANSIMFSVYDRLPFGSDFGQYKRAVPGYNVAYIDGFGYYHTRLDNPE
                     KISLASLQHHGDYALGLALHLGNMDLNDFYEPNATFFNPLGSYMIVYPQSWDNAFTFA
                     ALVLLVIALVINLKKGGIRIFRFLAALLVTVLTFILAALPGLLSLIAFVVYREASLYQ
                     NTLYCLGIIVAGMGIILLIYGLTTKLLRATELLGAVLCFWALLLYPAHSWLAGGAHIV
                     IWPMIFGSVLLLVLGLLYREGKDLQPGTVLLLTLPLLPVLAFLVPLLQMLSFTLTPMG
                     AVLINLFLVIIAGLFATQIQLLVKKRFLLAGGTVFLAGLLFFGWGLAANRPGPDNSRL
                     NSLAYAVNFDSGEAWWLSADRTTREWFKDIVGRELPKGLFENESPLDEWTSQFFPTTD
                     RAPAPEFRRGDMRPYLKAKAPDPNFEPFTMEVLEDRREAGKSRVTMHIASPRLAGEIA
                     LKKVSAGAVYSAALDGEPIAGKDESWSFHMNFLPARGATLTLEVDADDALRFFAREES
                     WVLPVFEQYKPRTASMAAEPNRVIDYYSRLHSEHCYSLATVVPKALPPEIAPEGASAD
                     ETGTEEGVSAETPDAGGGGEVESLSETEDETPDSEQTESAAT"
BASE COUNT         3183 a         3145 c         3382 g         2848 t
ORIGIN      
        1 taatactgag ctcataatgg cataccagct caaagacatt cagaaaatgt tcaactaact
       61 cgactcacgc agtccgttaa agattaaact atccgtgctc tcaggtttgc gagcaacgca
      121 aggaacctct atggcttcag gaagaaaaga aaagcaagtg gctgttccta aagaaaaaac
      181 cgcccgaaaa gacgagaaaa aaacttcagc aaaggcttct tccggagcga aagaagcccc
      241 cgtaaaaaag aaggcaacag cggtaaaatc tcatcctgaa aagaaagcgg caaaagtgcc
      301 caaagcaggt gcggaaacgc ccggaaaaaa ggcagatgca gaaaaaaaag taaagaaggt
      361 aattcaagcg aaaccttctg ctgttaaagc agccgacaag cctgcaactg caaaaaagcc
      421 ggcggcagcg gtaaagcctg cgcctgagaa gaaagcgtcc cgaacaaaaa cgcctgctgt
      481 cgctgcagcg caaacaccaa aagaggacgc agctccggac aaggcagtcg cgaagagcag
      541 ccccgccgca aagaaaacac cgcctaaagc agcgggtaaa acgacttctg ccgaggccgt
      601 cgaagttagc gaagcccccg tcacttcgga taaagcccga ttcaccacag tgaacaccca
      661 gtttttggtc agcgttgcac aggcgattaa gaatgcggtg ctgccgatga ttcaatccct
      721 gaaaggcagg gaaattgtca gtgatgcgcc aagcggcgat gtaacttttc aaattgacag
      781 agtggcggaa aaagcactga atgcctacct gcagtcagcc aatctgccac tgtcccttta
      841 ttcggaagac gtcgggtatt ccaccattac aagtaaaccc cctaaacacc tgcttattgt
      901 cgatcccatc gacggcacca gagcggcaaa aaatggcttt gaaggatgcg tcgttgccct
      961 cgcctccacc cgggtgctgg aaaggccgac cattggcgat attgagtgtg cctgtgttat
     1021 ggaactcaca gcagaccgct actttgtcgc tgagcggggt aaaggtgcca agtattatgt
     1081 gaatggcagt ctgaaacgtc cgaaactttc cgcaaatacc gatcttgagg cgctcacctg
     1141 gtccatgact gttcccgccc gaccggcaga actgattttt ccgacagcgg cgcggctcat
     1201 cgatctttcc agtttgaaag gcgggttttt cagctgcaac agcacctcct tcagcctgac
     1261 ccgtcttctc accaatcaaa tggatgcctg tgtagatttt gccaaccgat actatcgcga
     1321 tatgcctgag gaattgcgga aggtctttca ttttgcaggc aaaggaaaag taatcggcat
     1381 cgcgccttac gatatggcag cggcgctgct gatcgcccag gagtccggct gcatcgttac
     1441 agatgcctat ggtcagtctt ttgatgatct tctgctgacg gatacgacag agggtaatca
     1501 ccgctctttg attgctgccg caaataaggc gctccacgat aaattgctga ctttttttga
     1561 ggcccgcatc caacaatacc aggaaaaatt gaaactgttg cacaactagg gctgagtccg
     1621 ataattgcag gtgcactgca ggccgtcggc ccgatttacc gggaattaaa aatcctgaga
     1681 ggttattgtc gaatgagtgt agttctggta ccccttgctc ccggctgcga agaactcgag
     1741 gctgttacag tcattgatat tatgcgccgt ggcgggcttc aggttatcag cgcaggattg
     1801 aatcaaaccg ttgtttcagc gagccgcggc gttgtgctgc aggcggatca gcttctggaa
     1861 gaagcgattc tcaatgaaga ggtgtctgtt gttgtgctgc cgggcggtat gggtggtaca
     1921 cgggcgctca tgaatgcgcc cttctttacg ggctggctaa aaaaacgtgc tttggaaggc
     1981 atgcctgtcg ccgctatttg tgcagcgccg cttattctgg atcaggaacg gctcctgtcc
     2041 ggaaagcgtt ataccgccta tccgggtgtg ctggatgtcg ctctgtctga aaatgcctgt
     2101 tacacaggta ccgtgctgga agcggatgga aatatcatca cttcccgcgg accgggtact
     2161 gcaatggagt ttgcgcttta tcttgttgag ttgcttgccg gaaaagcccg ccgggcagaa
     2221 gtggaagcag ggctggtccg gtaagttttc agtaatctgt tttgtttttt tcgcaatatt
     2281 gctctttttt ggcaagaata aattcttgac aaagagggcg gaaaatggca ttataatagc
     2341 tgtgaatttt gtttttaatc cgcccgggga ttatctttta aatgcttcgt cggaagacgc
     2401 aaggagtgca ttttatgaag aacaggcgta cagttgccgg catgatcgcc ggtttgttcc
     2461 ttatggcgtt gatactgccc ggctgtccgc cgcaagtggg cgtggaactg gtgctttcca
     2521 aagatacgct tgatttcgga catgaccgca cggaagacgg actggctgtg catgtgaacc
     2581 acacaggcac ccgtacggag cctttggaag tttctgccga tcagccctgg attattccgc
     2641 agaattgtct ggatttcaaa gacggctgca tcagtcaggg gccttatcac gatctgcaga
     2701 tcagggtgcg tattgatcgg gataagcttg cgatgggttt gaatacgggc acccttcgtc
     2761 ttcacgcaaa tccggcgtcc cccaagacag tggaggtggt tgcggaagag attgcccagg
     2821 ctgattttta tgtccggcag agccaggtgg cgctgggaaa agcggttgag ttcatcgacg
     2881 caagtcaggt gtccgagaaa gccggaaggc ttgtttcctg gaaatgggag tttggagacg
     2941 gcaacgagtc aacggcaatc aatccggtgc atgtttacga acggccgggc gtttatgatg
     3001 ttcgtctgac catccagacc agtgaaaaca taaaacgcac ggtattgcag tcggcattga
     3061 tccgtgttga aaacccggcg gcacatgttg attttgatgt ctccaccaag gcgccgggtc
     3121 ttggggaaga agttattttt acagaccgtt cttcgctgga caacattaca gtcacaggcc
     3181 ggaaatggat ctttggcgat ggcacggaaa ctaatttttc caaagaaaaa acagccgttc
     3241 atcgctatgc gaaaacaggc gtctttacgg taacgctgca gctgaaaacc agccatggtt
     3301 tgcttgaatc ggttaaagaa aactttgtga tcgtccgttc cggcagcggt ccggttgcgg
     3361 atttcagttt tgaatccgat gcaggggcac tctacgtaaa agaggagatt cgtttttatg
     3421 atcgttccca cccggggaca ggcaccatta ccgaccgcag ctggaatttc ggcgatggta
     3481 cgatcagcaa tgaagcatct cctgtgcacc ggtacaacgg caagggcgag tttaaagtaa
     3541 cgctgagcgt gcagacagaa aagggataca gctcaaagga aaagacgcta acgctcactt
     3601 accgtgctcc caaggcaaat tttaccgctg cgcccaccaa gcagaaagtg gatgctccaa
     3661 tccgcttcac agatcggtct gttcccggtc atgacggcaa tgtggcgtgg gcgtgggatt
     3721 tcggcgacgg taacaccagc acggtgcagc atccgaccca tgtctatacg aaaaaaggac
     3781 tgtatactgt cacgcttact gtgacgggcg gcgatccgga tggattgacc gacacattca
     3841 cccgggaagg gtatatttcc atagttgata caatagacac aggcgagggc gaggacgaag
     3901 aggacaacgg cctccttgat ctcgaacact atgttgccct taatgatggc gcctatacat
     3961 attccacgcc gcgggtagag ccgctccggg ttgacggaag gacagcaggt accgtctatc
     4021 tcattgataa aatgacctcc cagcggtgga accccgataa cagtgtccat ccggattatg
     4081 ttgaatggtt ccatcccatg accattttcg agcctgctgt taaactgacg aaaacagcca
     4141 tgttgttcat tgatggcggc agtcgcacct ctccggcgaa agtggagcct gcttttgcgc
     4201 ggatgtctgt gctgtccggt accaccatcg ttcatattcg caatatcccc tgccagccca
     4261 ttgtttttct ggatgaagtg atccccgccg ggcggcagga taattacagc ggagaagata
     4321 tcatcctgcg ccgccgtacc gaggacgata ttatcgccta ctcttacgat gagtatttga
     4381 aggcatatcg ggaaacagag ggtaagccga atttccagtg gcccgctctt ttccccatgg
     4441 ttaaatcagc ggtgaagggc atggatacgg ctgaagatgt cctcgccaaa aatggcatta
     4501 cactggacgg ttttgtcgtt gccggcgcat caaaacgggg ctggacaacc tggctgaccg
     4561 gtgctgccga ctcccgtatt aaagcgatcg cccccattgt gattaacgtg ctgaatatgg
     4621 atgagcatct ggcacaccac cgcaagtcgt atggctactg ggcacctgcc atttatccct
     4681 atgcgcagga aggtgtgttt gatcagctgc ttcccaatga gcaagacgga acgatttcga
     4741 aagaggcgca gtcgcttctg aaccggattg atccctatga gtatgcgctt aaaggcagct
     4801 atccgatgcc gaaatttatg atgaacggca ccggcgatca gttctttgtt cctgactcga
     4861 cacagttcta tttcaatgac cttaaagggg ataaacatct ttgctacatt ccgaatgcag
     4921 gtcatgatct tggcggcatc gacggggtgg acatttctga tcccgaaaat cccctgggta
     4981 aatttttcgc ctggtatctc agtgtgaccc aaagagaagc acttccggga tttaccgaaa
     5041 gtttcgagca ggacggggct attgtcgtcc gggtatctcc tcaacggctg cctgtccggg
     5101 tgcgtctgtg tcaggcaacc tctgataaga gggacttcag gaatgaagtc atgaaagaac
     5161 agggcgttga gtggacttat accacgctgt cgccaacttc cggcagcaca gtgtatcgtg
     5221 cactgccgcc cgcacctaag gccgggcatt acaccgcctt ctttattcag ctggaatatg
     5281 cgaacacaag cacgctggca attccgggta ttcctgttcc caatctggtg ttcacaaccg
     5341 gtgtccggac gcttcctatt gtgaacggcg aaattgttta tcctgaattt acagggtatc
     5401 tcgccaatac cgagcggccc gatgcagtac ccttcagcga aactaaactt cctgtcgctg
     5461 tggtgtatgg cacgccttat gaaatggggc gctattacgg agaagtgctt ggcgatcaga
     5521 tcaacgatgc tgttcctgca atcatcgcgg catccggact ttctcaggaa gtgctgctgg
     5581 atatctggga agagcttgaa ggaaatgttc tggataaccg actgcaggaa gagatacgcg
     5641 gcatagcacg ggcgcgtaaa gtgaccgtga ccaaagacat gctcaatatg gcacatgccg
     5701 ccataatcca tgcgtccagc gcttccggta tttattcgac ggcggcatac ggcaatctga
     5761 ttaaagacca ggcgccgagg attcattcga tgcacagcgc cacggtgaat gagtctatgg
     5821 gattgaagat agcgccttac cagtgtgctg tcatgtacat tccggatcag ggcattccgc
     5881 atacagtgtt tacctatgca gggctgacct tcgggcgtac aggcattaat ctgggcggca
     5941 tttcaggtgt tgatatcccc aatcttacca gcgatgccga cttgtctacg ggtatgccgc
     6001 tgctgcgtga agcactctat gattccctta acttgaggga agccgttgcg acagcgcgca
     6061 ccggtctttc ccgcggtacg gcgctggtat tttctgacgg gcgcaatgag tatcgttctg
     6121 cttatgtacc ggcagcaccg ggtatcacgc gttttgatat gacgctggat cagatgaggt
     6181 accccgtgcc tggcctggtt ttcggcacct cgcaggatcc ctatgagacc ttgagtttcc
     6241 tgcttaattc caccatcagc accttcacct cggaagtgct gttaaatgta acgaacaaca
     6301 atgctttgac ggattcaaag cgcaatacgc tcaatgttgt ctatgagggc gagccgctga
     6361 cagttgttat tcagactgcg agcagcagtt taagcgcccg gtgggatatc cctgaataca
     6421 tgaatatgca gttgctgctt ccctgagtcc ggactcaggg caggttgtac cctgcaaaat
     6481 gaaactgcct ccggcagcag atgacactgt tgccggaggt tctttttgtt caggtacagc
     6541 cttttttccg acggctcccc gtgaaaaaaa gagggttctg atcaggctgc gattttacag
     6601 ctggatgcgc tcaagactga cgttagcagc ctggctgtaa tggcggtgca gcatttctgt
     6661 gagacgaaag aaatccttgt cggatcgact gtggatatgg aaggtatact gaagcctgtg
     6721 tccttcctcc aggctttgta tggctgtagt atgcacttca aacctgcctg cctcttcgat
     6781 atgtcggaga attcgggaga tatctccttc cgaagcggga agctgcatgc tcagcagaaa
     6841 ggatccgtac tgaggaagca gaggggagag cgctgcgaag cctatgacaa cagccagaac
     6901 gaaagcggtg gcaaggcagg ctgtgatgag gtatcccgcc cccacagcaa tgcccagcgc
     6961 tgcagtgaac cacaccgttg cggccgttgt gagccccagt ttatcatgcc cggatgtgat
     7021 aatggttcct gctccgagga aaccgatgcc ggtcataatt cccgccgcaa tccgtgcctg
     7081 gtcaaagggc tgtgcggctg ctccgaaagt ttcatacatg tatgcgctca cgagcatcag
     7141 gagcgtggat ccaagcgcta ccagcacatg agtacgcaga cctgcactcc gccctttacg
     7201 ctgcctttcg atacctatga cggatgcgag gaagagggat gcgagaagtt tgaacagcac
     7261 ttccagccag ttttccgctt ctatatgaaa gcctgtgtct gtcagaaagt actccatctc
     7321 ttttgatcct ttgccccgat ccggcttcac tctatcagtg tacgcatagg taccccggga
     7381 cttgcaccgg tgttaggcga cgcaggcagc gccggaacag gcaccccgga atcaggcacc
     7441 gcatttggcg cgggcagccc cgtcttcaga acggtttccc gccgatcaag ttcggcttgc
     7501 ggcgcctgaa gatcccgggc agccttaagc cattcttctg ctttttcttt gtcctgccgc
     7561 acgagagcca gatctgcgag aaacagtctg gggcgaaatg aatcaggcat cagctccgcc
     7621 gcttttaaaa accagctttc ggcctgatca agccgtttct gctccaaaga aaaaacagcc
     7681 attaattctt cagcagaaaa atcgggtgac tttccgggct gattcaaata gacaaaggca
     7741 gcatcccagg cctgagagaa agcagattta tgggcaagat caatccaggc cttgtcatcg
     7801 gctgctttta aggcgcccca tttctcaaca aaattctttt cggcgccttt cattgcactg
     7861 agaacgccca gcaattccca ggctttcggg acagaggaat cccgtctgag ggcaaattcc
     7921 agccagtaaa aagcctgaag atgatttccg gcaagcaagt ggctttctgc aaggctgaca
     7981 aacccttcca tggatttggc atcagcctcg attttctttt gggactcttc tgccagggcg
     8041 tcaatggtac tgcggatctt cagactctgt tcgcgcagtt gtccttcagg agattgagga
     8101 tgcagcccgg acagccgtga aaaatgaagg gcggcatttc gggcatcacc gaggcgcata
     8161 tgggtcaggg ccgattgcag ttcctccgcc tgcgaaaggt ccctgtgtct tgaaatactt
     8221 tccagcagct ccagaataaa agaggcattt tctcctggct cctgaagtct tgccattccg
     8281 gctgcacctt ccagcccgtt ttcagtgaaa gaaaagtgct cccgcagttc ggcatagtgt
     8341 gcaagagctt cgtcaggagc attcgtttct tccagaactc gcgcaaaggc aagcttatat
     8401 atgccctcac cgctcaagtc gggcggcgat gttttccaga gtgtcgctgc ctggcgcaat
     8461 tgatcgattc gctcagccgg aatatcctct ttttctgcaa gtgcaataca ctgcaaagca
     8521 ccgcgcatga ttttatcggg agcatccgtt ttttcgatac ggctcaaggg ttcccaaaat
     8581 gccatagtct gctgaaagga aatgacagca cttgcaatga gcagaaggtt cagtaaaaca
     8641 agcagtatgt tttttaaaat tttactccgg aagagaagaa gcgtccttat cagaaaaagg
     8701 atgcatacca tgaacagcag atgatagggg atatataaac gtcggaaaga aaatgagtct
     8761 gaaaagcaga caactattcc gagggccgcc aggacgcctg tgacggagaa gagcatgatc
     8821 cagtcgggtg acattcttgt ttcacggtgc ttttcaagcc gtgtcgcatc aagcagcaga
     8881 agaacaagag gcagggcgat gagcccgtaa taggatcctg ccgccaacag agcgaaagcg
     8941 agtgttgcga aaaggtgcag cggtctgcga cggtccggat cttccgtaac ggcaaggtag
     9001 gaattggcac aaatgagggc aaagaaagtg cccagaagct gcgacagacc ggaggcacct
     9061 gccagcagcg gcggcacagt cggaagcacg cagaaaagaa agccacccag taaaggcggg
     9121 ataacagatg ttggttttcc tgtccagcgt cgtacaattc cgaagaaata aagcgcggac
     9181 agtacatgaa gcacgaggca aaagagacga aggttcttcg cggatccatc tgagagagac
     9241 cacaaaaaag ttaatacggc tcttgcggca ggcgcattgg gcagctcctg ctggagtgcg
     9301 gcactgctga tcggcttgtg cagataccgg gggagtacga gcagctccag atctccgccc
     9361 tgcagcggga cgccggtcat gctgaaataa aaaaagaatg tgatgacacc taccagaagc
     9421 aggctgatca ggtatagatt gcgcggttcg gcagcaaggg cggctgtgcg tacaggtttt
     9481 tcctgagtat tagacgatat catgataaaa acgacccgaa tgttttttca gagaaacaga
     9541 tcgaattgcc ggggctgtgc aggtgaaaag accccgaatc ttcttatcat gattgtagca
     9601 ggtcgggcgt aaaaaaggct aaagcggaga cacgtccgga cgctatggta cgcaccccgc
     9661 ttttttgctt cagaaaagaa gccgtaaaca gcgcaaggca tggatgtctg aaaagatcat
     9721 gttgccgctg attcagtctg ttctgagtcg ggcgtttcat cttccgtttc gctcaggctt
     9781 tccacctcgc cgccgcctcc ggcatcaggt gtttcagcag acacaccctc ttccgtgcca
     9841 gtctcatcgg cggatgcgcc ttcgggagcg atttccgggg gtaatgcttt cggaacgaca
     9901 gtcgccagcg aatagcagtg ttcgctgtga agccggctgt aatagtcgat aacacggttg
     9961 ggctcggcag ccatagacgc ggtgcgcggt ttgtattgtt caaacacagg cagaacccag
    10021 gactcttccc gggcgaagaa gcgaagtgca tcgtctgcgt ccacttccaa tgttagcgtg
    10081 gcaccgcggg cgggcaggaa attcatatgg aaagaccagg attcatcttt acctgcgatc
    10141 ggctcgccgt ccaatgccgc actgtatacg gcacctgctg acactttttt aagggcgatt
    10201 tctccggcaa gacgcggtga cgctatatgc atggtgaccc ggcttttgcc tgcttcccga
    10261 cggtcttcaa gaacctccat ggtgaagggc tcaaaattgg gatcgggtgc ttttgccttc
    10321 agatacgggc gcatatctcc cctacggaac tccggtgccg gcgcccggtc tgttgtcgga
    10381 aagaactggg atgtccattc atcgaggggg ctttcatttt caaaaagccc tttgggaagc
    10441 tcacgtccta caatatcttt gaaccactcg cgggtagtcc ggtcggcgct gagccaccac
    10501 gcttcgccgc tgtcaaaatt cacggcatag gcgagggaat tcaggcggga attgtccgga
    10561 ccgggtctgt tggctgcaag cccccaccca aagaaaagca gtcctgcaag aaaaacggtg
    10621 ccaccggcga ggaggaagcg ttttttaacc agtaattgaa tttgtgtggc gaaaagcccg
    10681 gcaatgatta ccaggaaaag attgatcaac actgcaccca taggagtcaa ggtaaaggaa
    10741 agcatctgca gaagcggtac caggaatgcc aaaacgggca ataaaggaag agtcaggaga
    10801 agaacagtac cgggctgcaa gtccttgccc tcccgatata agagtcccag caccagaagc
    10861 aggacggatc cgaaaatcat cggccagata acaatgtggg cgccccctgc aagccagcta
    10921 tgggcaggat agagaagaag cgcccaaaaa cagagtaccg ctcccagaag ctcggtggcg
    10981 cgcaggagtt tggttgtaag tccgtaaata agcagaataa ttcccatccc ggcgacaata
    11041 atacccagac agtagagcgt gttctgatac agacttgctt cgcggtagac cacaaaagcg
    11101 atcagcgata aaagtccggg cagggcggcc aggatgaagg ttaagactgt caccagcagg
    11161 gcggccagaa atcgaaagat tcgaattccg ccctttttaa gatttatcac cagcgcaata
    11221 acaagcagca ccagtgctgc aaaagtaaag gcgttgtccc aggactgggg atacacaatc
    11281 atataggagc ccagtggatt gaaaaaagtg gcattgggct cataaaaatc attgagatcc
    11341 atattgccaa gatgcagtgc cagccccagt gcataatctc catggtgctg caggctggca
    11401 agagagattt tttccgggtt gtccagacgg gtgtggtagt aaccgaatcc gtcgatgtag
    11461 gcaacgttat accccggtac agcacgcttg tactggccga aatcgcttcc gaatggaagc
    11521 cggtcgtaga cgctgaacat aatggaattg gcgcgggcag agacgccgga ttttttcagc
    11581 tcacggatga gtttgccgtt gttctgagag gtttcaaaca tgagagcagg accgcagaca
    11641 cctctggttt ccaaacccag catgacacct acgtcattaa accaggggtg gcgcctgaat
    11701 gccttggcac cattggcacc cacttcttcc tgatcggcaa aaacaaagat caaatcgttt
    11761 ttaaggggag cgcgggcctg caatgcgcgc gccgtttcca gcatggtcgc aatgcctgaa
    11821 agatcatcgg cagcaccgga accgtaaggc actgagtcaa aatgcgcgtc catggcaaag
    11881 gctttggtag agtctgtgcc gcgtatacgc ccgaggatcg cacgccccca ctcagcagaa
    11941 ttccgtcctt cgtgaatgaa gggtgtatca atcacttcga tttccaggcc cagagcagca
    12001 gcctgatcca gaaggtactc aaaaacccga tcattggcgt gagagctggc agggtggggt
    12061 tctttagcca tgattttaat atgttctaaa gcacgctctg ccgaaaaatc ttcctttgga
    12121 gcctgcgcat tcagaggggc aggcgcctgc gccatccacc aggacaggaa tcctgttccc
    12181 agcaataaaa aaaggatcag cagagcagaa agttttcctg ttttaatttc agaaggcatg
    12241 ggtactcctt ttgcatgtta ctgcagtaca ggttgcttct gaaattaaag actgttcaga
    12301 tactgaaata gtttcagatc gtgaaaagga aggaatgtgg gtgatgcgca ggaaaatgaa
    12361 atactaaata caagaaattc cgaggctctg caaaaaaaag attatcctcc ctccaggggt
    12421 agaggaggaa ataacgggca ttttgcccgt gcgctcagta aataaaagga gtttctatgt
    12481 taacgggcgg aagattaacg gcatagcaga accagttaac cggcatcagc acaatttcca
    12541 caaccatcat catgatcc
//
LOCUS       DAOPWB010000091        12369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5207_length_12369_cov_5.740377, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000091 DAOPWB010000000
VERSION     DAOPWB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12369
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5207_length_12369_cov_5.740377"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3527
                     /locus_tag="PKN92_09000"
     CDS             <1..3527
                     /locus_tag="PKN92_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ48969.1"
                     /translation="YFGLEKLIRPQRHYQENWRDPAPLVFVEKTHPVFHGLYTGEEEI
                     PLSRGGCRAVADFYWGGPGEGMVLANSPAGVHRPLVEYASGKGRLLTFGWRWPDYADL
                     DNPDRDALLRLTCNMLNYLGTPEQWQPFRIRSEFPYTRLAGTGMIDPLRFEPLRRSIS
                     HLIDEYGSHYPQGEEYLTRLDELVRIYEEAQRAGDRATLEMVADRFEALQREAMLANP
                     LLDFEKILFIKRDAEQLGLPDNSYGNEYLAPTGYNNSLQALSYKTNEAPYTVFTPEND
                     VFIGDLDLHYDGAKMLLSVPDLSGKWGVGELDLESGTLRMLPLIPDEDVHNFDACYLP
                     DERIVFTSTAPYIGVPCVGGRSKVTNLYLLEKNGAIRRLTNDQDHNWCPAVLNDGRLL
                     YLRWEYADIAHAFMRILFSANPDGCRQMEYYGSNSFWPTAMFFARAVPDHPSRFVAIV
                     GGHHDAHRQGELILFDTSLGRHEADGVVQRIPGYGKKVEPVILDGLISASWPRFLHPY
                     PLSDSYYITACQPDKNALWGIYLVDRFDNFTLLHEEESFAMLEPTAWRKTKRPPVIPD
                     CSVPGAEESVIQLADVYHGPGTAGIPRGTIKKLRIIGYEYTFHNFGCEPDRVGMDGPW
                     DVRRILGTVPVEEDGSAHFAVPAMTPVAVQPLDEDGKAVALMRSWFTAAPGEALSCVG
                     CHEPQNSAAPPQYTPQAFKRPPDNIAPWYGPARGFSFQREIQPVLDAHCVTCHDGAKH
                     ENIPDFTDKPVQKYNSAFQLHFSPSYMALRSWVHTPTLESDAHMLPAKAFHADTSMLI
                     QILRDGHYSVQLDEECMDRLITWIDLNAPFHGSWQECVAYSPEKSAAALKGAEQRREL
                     HKRYTGIDEAFDALSEKAVITHSTASDPSDCLPREYDGTVQETAGILTHNANSTTRTI
                     AITPELSLDFVLIPPGEFIFGSDSGYPNEAPARKVTIEKAFYMGKFEISNAQYAVFDP
                     DHDSGLETGEGYQFGDDERGFPLNRPEQPVVRISWNQAVAFCEWLSARTGLRFSLPTE
                     EQWEYACRAGTNTAFWYGSLDTDFAATANLSDATHYEVYYPHVPSIHPPWRPADTRFN
                     DTWRVSAPVDAFQANPWGVHNMHGNAAEWTISTYYSDSNGTEERKVVRGGSWMDIPKR
                     ARSAFRLHYNASQGVHDVGFRVVCSTLP"
     gene            3771..5930
                     /locus_tag="PKN92_09005"
     CDS             3771..5930
                     /locus_tag="PKN92_09005"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNZ48970.1"
                     /translation="MTPAAQDAFAAQQTAVPFFFSEIEKRDGRVVAFQSSKITAALAK
                     AGQATGEFSEETARQLTMRVLSLCQQAESDAVPTVEAIQDVVEEVLLMSPFKKTAKAY
                     ILYRDQHARVRELINKADVELIDRYLDQMDWKVRENSNMAYSLQGLNNYISSEVSKTY
                     WLNKIYTSDIREAHSRGDLHIHDLGLLSVYCVGWDLQDLLRSGFCGAPGKAESAPPRH
                     FRAALGQIVNFFYTLQGEAAGAQAFSNFDTLLAPFIREDRLTYEEVKQSLQEFVFNLN
                     VATRVGFQTPFTNLTMDLQPPAALAGQPVIIGGTSQDTVYGDYQHEMTMLNSAFLEVL
                     AEGDAKGRVFSFPIPTYNITPDFDWERPGLDKLWEVTAKYGIPYFANFINSDMSADDA
                     RSMCCRLRLDLRTLEKRGGGLFGANPLTGSVGVVTINMPRLGYLAQSEEAFFDRLNRL
                     MDLAKESLETKRKILEAFTAKSLYPYTKFYLRDVYNRFNSFWHNHFSTIGLVGMNEAC
                     LNLFGKDIGTEEGSAFACRVLDHMRDRLAEYQEETGSLYNLEATPAEGTAYRLARLDR
                     KYNQGILFANHKEVSEGAEPFYTNSTQLPVNYTDDIFEVLDRQDPLQTRYTGGTVLHV
                     FLGEAAADPQAVKTFVKKVCENYSLPYFTVSPSFSICQTHGYLNGEHPVCPECGSTTE
                     VYARIVGYLRPINQWNEGKQAEFDIRRHYSVDASPAK"
     gene            5938..6519
                     /locus_tag="PKN92_09010"
     CDS             5938..6519
                     /locus_tag="PKN92_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNZ48971.1"
                     /translation="MLFGGYQPISLSDFPGRVAAILFTQGCNFRCPYCHNAELIPLCA
                     HPDKTFGRKEALALIQSRQGLLEGVVITGGEPTIQPDLYDFLCELRTLSLKIKLDTNG
                     SNPDCLARLLRDNLLDYVAMDIKAPECKYDLLSGSAADYPSIQKSIALLAGSAIDCHF
                     RTTFMQPLLNDDDIESIRTSLPENIRYIVQECK"
     gene            complement(6569..8665)
                     /locus_tag="PKN92_09015"
     CDS             complement(6569..8665)
                     /locus_tag="PKN92_09015"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNZ48972.1"
                     /translation="MQTKSFLPGLVVAILFSHFAASAPLDTVGNRTVLIDLLHPLPPS
                     VQFHDSHAGKVTESGLQILFKPADWPGIYVHAPEDTWDFSGHFGMGFTVHNPSAAPAV
                     ICLRVDNDGADGTNHCNNLESEIAPGETKDVCMAFSRNDKEFFWGMRGVPPVDAPLGK
                     GAPLNLKEITGFQIFLAHPAQDTTLALKQVFLFGGSHADYINAHMPFVDAFGQYKHGD
                     WPGKLKEEGDFAQRLEEERTLLEATPALAERDSFGGWLAGPQLAGTGWFRTEKIDGKW
                     WLITPEGRLFLSLGINCVGFSEYTFAEQRDAWFEYLPDKNDPIFSQAFTVLHGVHSMA
                     DVIGGSGSAFNFYAANMIRKYGKDWKTFGREMAYKRLRAWGFTTIGNWSPPFMLETSP
                     VPFTSSATTGNAPRLQAAEGYWSKICDAYHPEFRESIEPEISAMTAKYSGNPLCIGYF
                     VDNELSWEGVIGGVLASNAEQPARQAFLKQLQEHYKDIQSLNDAWESDFSSWDAIERP
                     SKRTKAFKDDTDSFLYQFALQYFTTVRDILKEKAPNQLYLGCRFSPATEPAVRACAAV
                     ADVVTFNIYSRQVTPDRWAGEHDLNKPLLIGEFHFGALDRGLFHTGLVATRDQDDRAT
                     HYKNYVRSVLEHPGFVGCHWFQFIDEPLTGRSLDGENYNIGFLTVTDTPYPELVEAAK
                     EIHGKAYEIRFNAGRE"
     gene            complement(8753..10597)
                     /locus_tag="PKN92_09020"
     CDS             complement(8753..10597)
                     /locus_tag="PKN92_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48973.1"
                     /translation="MNSKMLFLLWFSALFTLPFSGLYAQEENTLLYSARDFGVVPDTE
                     EDMGPVFRALIKEAIDSGRPSVILLEKGCYRISGGDRHYSALVIEKARNMTVRGEGDD
                     TELIFTDPRQGAFFVGQSENVFIESLSIDYDPLPFTQGTVLALSRSEGWFDLAVQENF
                     PLLSETWFSDAPKPHGQWGMIFDRNTKTVKAGAADFIFMEQWKQISRRVWRMYPVDSQ
                     KDRLTDMQMGDRFVQLARYGNGGALFFWRSFHCGASQVTVYASQSVAVGSLESDRTQL
                     RKIKVYPKPESGRFLSTNSDGVHSQQDTTGPLIEACSFEGMADDGVNIYSRPCLMDAL
                     IDSNVIRIGTGGYFCEGDKVQIVDPVQGALLLESTIVRMDRQEDGRLRLTLKDPLVML
                     RPGIVDWQVYNLSRCGADFVIKDTVFMNHRRHGMMLKSTDGTVENICITNVGGFGIVI
                     GNDPEWPEGVIPARLAFSDIRIEHTGYSRWYGSIDRGAAIQILTRTLDGRMPLSRAVN
                     AIRFDRIAITEPPGAGIAADGAENIVIEDASVVYPEEQKLPRKCSALEFGNVGNLYLD
                     KVSVKAKGVPLQSGILFGNAAEPGEKGVFLNEYHFEGTAPAEAILDKR"
     gene            10717..12123
                     /locus_tag="PKN92_09025"
     CDS             10717..12123
                     /locus_tag="PKN92_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNZ48974.1"
                     /translation="MMSTISRRRFNQCLAAGAGLVLSQTLSSRIKAEETIRPNIVFLL
                     ADDMRWDTLGCMGNPVIQTPHLDRLAAEGQVFDQCRVTTSICCSSRASILSGQYVCRH
                     GIDDFAKTFTPEAFAQCYPALLRNHGYYTGFVGKYGVGTELPEKAFDFWRGFGGQGNY
                     ENFDEAGTFRHLNRITAEDSLAFLENCPKDRPFCLNVSFKAPHCQDGDARQFIHEEEY
                     NDLYADLIMPVPASNTVSYEDRFPEFFRDNNEARRRWKLRFATPEMQQESIRKYYRLI
                     TGLDRVAGEIRDKVDALGFGENTLFIFTSDNGFFLGDSGLAGKWYGYEASIRVPLIIY
                     DPRSSRKEQDVRHYIPVLNIDIAPTILELANIPIPDTMQGKSLVSRMQGEKSMWRSDF
                     FYEHNFHHPGIPKSEGVVSREYKYLRYHEQNPLYEELFDLNADPDEVINLAGKEEYEP
                     LLKRMRNRFDELKEECCR"
     gene            12180..>12369
                     /locus_tag="PKN92_09030"
     CDS             12180..>12369
                     /locus_tag="PKN92_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018258641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNZ48975.1"
                     /translation="MVSLTDSDSASDKVLSDLNRLSGASSPYLRQHALQPVAWQPWDE
                     EALLKAQLEDKPIFLSIGY"
BASE COUNT         3072 a         3263 c         3017 g         3017 t
ORIGIN      
        1 tctatttcgg tctggagaag ctgatccgac cacaacgaca ttatcaggaa aactggcgcg
       61 atcctgcccc gctagtcttt gttgaaaaaa cacatcctgt ttttcatgga ctttatacag
      121 gcgaagaaga gattccacta agcagaggag gctgccgtgc tgtagctgat ttttactggg
      181 gcggtccggg ggaaggtatg gtgcttgcca acagtcctgc cggcgtgcat cggcctcttg
      241 ttgaatatgc atccggaaag ggcagactcc tcaccttcgg gtggcgttgg cctgattacg
      301 ccgatctgga taatccggac agagacgctc ttttgcggct tacatgcaat atgctcaact
      361 atcttggcac tcctgaacag tggcagcctt ttcgtatccg cagcgaattc ccctatacaa
      421 gacttgccgg aaccggcatg attgatccgc ttcgatttga acctctccgt cgctctattt
      481 cgcatctaat tgacgagtac ggaagccatt atccccaggg ggaggaatat cttacgcgtc
      541 ttgacgaact cgtcagaatc tatgaggagg cacaaagggc aggggaccgg gcaactttgg
      601 aaatggtggc cgatcgattt gaggcgctgc agcgggaagc catgctggca aacccgcttc
      661 tggactttga aaaaattctt tttatcaaac gcgatgccga gcaattgggg cttcccgaca
      721 acagttacgg caatgagtat cttgcaccga caggttacaa caactcactg caggcgctgt
      781 cttataagac aaatgaggca ccttacactg ttttcacccc tgaaaacgat gtatttatcg
      841 gtgatctcga cctgcattat gacggagcta aaatgttgct gtccgtaccg gacctgtccg
      901 gtaaatgggg agtaggagaa ttggatctgg agtcagggac gcttcggatg ctgccgttga
      961 tcccggacga ggacgtacat aattttgatg cctgttacct tcctgatgag cgtattgtct
     1021 ttacttcgac ggcgccttat atcggagtac cctgcgtcgg aggaaggtca aaggttacca
     1081 atttgtatct tctggaaaaa aatggagcga tccggcggct gaccaatgac caggaccaca
     1141 actggtgccc tgctgtgctt aatgacggca gactcctcta tctgcgctgg gagtatgctg
     1201 acatagccca tgctttcatg cgtattcttt ttagtgctaa tccggacggc tgccgccaga
     1261 tggaatatta cggcagcaac tccttctggc ctaccgccat gttttttgcg cgagcggtgc
     1321 ccgaccatcc ttcgcgcttt gtggctattg tcggcggtca ccatgatgcc caccgccagg
     1381 gcgaattaat tctctttgac accagcctgg gcaggcatga agcagacggt gtcgtccagc
     1441 gtatcccggg ctacggaaag aaagtggaac ctgttattct tgacggactt atttcagcaa
     1501 gctggccacg tttcctgcat ccatacccgc tcagcgacag ttactacatt acagcctgcc
     1561 agccggataa aaatgcactg tggggcattt atcttgttga tcgtttcgac aattttacac
     1621 ttctccatga agaagaatct tttgccatgc tggaaccgac cgcctggcga aaaacaaaac
     1681 gcccgcctgt gattcctgat tgcagcgttc ccggcgcgga agaatccgtt atccagctcg
     1741 ccgatgttta tcacgggccc ggtactgcag gtattccccg gggcactatt aaaaaacttc
     1801 gaattatcgg ctatgaatac acttttcaca acttcggctg cgaacctgat cgcgtgggta
     1861 tggacggccc ctgggatgtg cgccgtattc tgggcactgt accggtggaa gaagacggat
     1921 ccgcgcattt tgctgttcca gccatgactc cggtagcagt acagccgctg gatgaggacg
     1981 gaaaagcagt agccctgatg cgcagctggt ttacagcggc gccgggagaa gctctgtcct
     2041 gtgtcgggtg ccacgaacca cagaattctg ctgcaccgcc gcagtatacc ccgcaggcat
     2101 ttaaaaggcc gccggacaac attgccccct ggtacggtcc tgcgcgtggc ttttctttcc
     2161 agcgggaaat tcagcccgtg ctggacgcgc actgcgttac atgccatgac ggtgcaaagc
     2221 acgaaaacat accggatttc acagacaaac cggtacaaaa atacaactcc gcatttcaac
     2281 ttcatttttc accctcctac atggcgctgc gcagctgggt acatacgcca acattggaaa
     2341 gcgatgccca tatgcttccc gctaaagcct ttcatgcgga tacaagcatg ctgattcaga
     2401 tactcagaga cggacattat tccgtgcaac tggatgagga atgcatggat cggcttatta
     2461 catggattga cctgaatgcg ccttttcatg gctcgtggca ggaatgtgtc gcttatagcc
     2521 cggaaaagag tgctgccgcc ctgaaaggcg cggaacaacg cagggagctg cacaagagat
     2581 atacaggcat cgatgaggcc ttcgacgccc tgtctgaaaa agcagtcatc acacacagca
     2641 cggcatcgga tccttcggac tgtcttccaa gagagtatga cggcacggtc caggagaccg
     2701 ctggcatact tacgcataac gccaactcaa caacacgcac aattgcaatc acgccggaac
     2761 tgtctctgga cttcgttctg atccctccgg gagagtttat tttcggatca gacagcggct
     2821 atcccaatga ggcacccgca cgaaaggtca ccatcgaaaa agcgttttat atgggaaaat
     2881 ttgaaatctc caacgcacaa tacgccgttt tcgatcctga tcacgacagc ggactggaaa
     2941 caggggaggg ataccagttc ggggacgatg agcggggctt cccccttaac cgtcccgaac
     3001 agcctgttgt ccggatttcc tggaatcagg ctgttgcttt ctgcgagtgg ctgtctgccc
     3061 ggacaggtct ccgcttctcc ctgccaactg aagagcagtg ggaatatgca tgccgggcag
     3121 gcacaaacac agccttctgg tacggatctc tggatactga ttttgctgcc accgccaatc
     3181 tttccgatgc gacgcattat gaagtctatt atccccatgt cccctccatc catccgccgt
     3241 ggcgacccgc cgacacccgt tttaacgata cctggcgcgt gtctgcgcct gtggatgctt
     3301 ttcaggcaaa tccctggggc gttcacaaca tgcacggaaa cgcagccgaa tggacaataa
     3361 gcacctacta tagcgacagc aacggcaccg aggagcgtaa agttgtgcgg ggcggttcct
     3421 ggatggatat cccgaaaagg gcgcgctccg ctttcaggct gcattataac gcctctcaag
     3481 gcgtgcatga tgtcggcttc cgtgttgttt gctcaaccct gccctaatga aggactcgag
     3541 agcctctttt tagcagcgga gcagattcag gaaagattgg gaataccgct gtgctccgga
     3601 ggttttattg gtattctttc ttttatgctt tacatacagt atattgtggt ctttgcgctt
     3661 cgatacactt gactttgtgg tagacagtgg ttaaaataag gcaatgattc agtctgcccg
     3721 gtgctttctt gggcgatggt accgggctga aaagcgagga gatctatgcc atgacccctg
     3781 ccgcacagga cgcatttgct gcgcagcaaa ccgctgtacc tttctttttt tcggagattg
     3841 aaaaacggga tggaagggtt gtggcttttc aatcttccaa aatcactgcc gcactggcga
     3901 aagcagggca ggcgaccgga gaattttccg aagaaactgc cagacagctc accatgcgtg
     3961 ttctcagcct ctgccagcag gcggaatcgg atgccgtgcc tacggtggaa gccatacagg
     4021 acgtggtgga agaagtgctg ctcatgtcgc ccttcaaaaa aaccgccaag gcatacatcc
     4081 tttatcggga tcagcatgca cgggtccggg aactgatcaa caaggcagac gttgagctga
     4141 tcgaccgcta tctggatcag atggactgga aagtgcgtga aaacagcaat atggcatatt
     4201 ctctgcaggg actgaacaac tatatttcca gtgaggtaag caagacttat tggctgaata
     4261 aaatttacac cagtgacatc cgcgaagccc acagccgtgg tgatctccat attcacgatc
     4321 tgggtctgct ttctgtttat tgtgtaggct gggaccttca ggatcttctg cgttcgggtt
     4381 tctgcggcgc accgggcaaa gcggaaagtg cacctcccag gcatttccgt gccgctctgg
     4441 gacaaattgt caactttttc tataccctgc agggagaggc ggccggtgca caggctttca
     4501 gtaattttga tacccttctc gcaccgttta tccgcgaaga ccggctcact tatgaagaag
     4561 taaaacaatc tcttcaggaa tttgtattca acctgaacgt cgccactcgt gttggttttc
     4621 aaacgccctt cacgaacctg accatggatc ttcagccgcc tgcagcactc gcaggacagc
     4681 ctgtgattat tggcggcaca tcgcaggata ctgtatatgg agactatcag catgaaatga
     4741 ccatgctgaa cagcgctttt cttgaagtgc tcgcggaagg cgatgccaaa ggacgcgtct
     4801 tcagctttcc tatccccact tacaatatta cgccggactt cgactgggag cggccgggcc
     4861 tggataagct ctgggaagtg accgccaaat acggcatccc gtacttcgcc aacttcatca
     4921 attcggacat gtctgccgac gatgcacgga gcatgtgctg caggcttcgt ctggatctga
     4981 gaacgctgga gaaacgggga ggaggtctct tcggtgccaa cccgctcacc ggatccgtag
     5041 gtgttgtaac catcaatatg ccccgcctcg gctatcttgc tcaatcagaa gaggcgtttt
     5101 tcgacaggct gaacaggctt atggatcttg cgaaggagag cctggaaacg aagcgaaaaa
     5161 ttctggaagc ttttactgcc aaaagcctct acccttatac aaaattttac cttcgcgatg
     5221 tgtacaatcg attcaattcc ttctggcaca accatttttc aacgatcggg ctggtcggca
     5281 tgaatgaggc atgcctgaat ctattcggaa aagatatcgg cacggaagaa ggttccgctt
     5341 ttgcctgccg tgtgctggat cacatgcggg atcgccttgc cgaatatcag gaggaaacgg
     5401 gcagtctgta caatctggaa gccaccccgg cggaaggtac tgcctatcgt ttggcacggc
     5461 ttgacaggaa gtataatcag ggtattcttt tcgcaaacca caaagaggtg tcggaagggg
     5521 ccgagccttt ttacacgaat tccacccaat tgccggtcaa ttacaccgac gatatttttg
     5581 aagttctgga ccggcaggat cctttgcaga cgcgttatac aggtggaaca gtgctccatg
     5641 tctttctcgg agaagccgcc gccgacccgc aggccgttaa aacttttgtg aaaaaagtct
     5701 gcgaaaatta ttcattgcct tattttactg tgtctcccag tttttccatt tgccagaccc
     5761 acggctatct taacggcgag cacccagtct gccctgaatg cggaagcacg acagaggtct
     5821 atgccaggat tgtcggatat ctgcgcccga tcaaccaatg gaacgaaggc aaacaggccg
     5881 aatttgatat cagacgccat tacagcgtcg atgcttctcc ggccaagtaa tccagacatg
     5941 cttttcggcg gctatcaacc gatttcactc agcgactttc cggggcgggt agccgccatt
     6001 ttatttacgc agggctgtaa tttccgctgt ccctattgcc acaatgccga gctcattcca
     6061 ctgtgcgcac atccggataa aactttcgga cgcaaggaag cacttgcgtt gatccaaagc
     6121 cgccagggtc ttctggaagg cgttgtcatt actggcggag aaccgaccat ccagcccgat
     6181 ttatatgact ttctttgtga gctgcgcacc ctcagcttaa aaatcaaact ggacacgaac
     6241 ggcagcaacc ccgactgcct cgcccggctg cttcgggata atcttctcga ttacgtcgcc
     6301 atggacatca aagctccgga gtgcaaatac gaccttctct caggttccgc tgctgactac
     6361 ccctccatcc aaaaaagcat tgcactcctt gccggctctg ccattgactg ccacttccga
     6421 accactttta tgcaaccgct tctcaatgat gatgatattg aatccatccg taccagcctg
     6481 cccgaaaata tcaggtatat cgtgcaggag tgtaaataga atggttcgtg cgcaattccg
     6541 ttcaagcggt tcagaataac aggccttctc attcccgccc tgcgttaaag cgtatttcat
     6601 aggctttacc gtgaatttct ttagccgctt cgaccagctc aggataaggg gtatcggtta
     6661 ctgtcagaaa cccgatgtta tagttttcgc cgtccaaact gcgccccgtt aagggttcgt
     6721 caataaactg aaaccagtgg catccgacaa aacccggatg ctccagaaca ctccggacat
     6781 agtttttgta atgggtcgcc cggtcatcct gatcccgggt tgcaaccagt cctgtatgaa
     6841 acaggccccg atccagtgcc ccgaaatgga attcaccgat caggagcggt ttattcagat
     6901 catgctcgcc tgcccatcta tccggcgtta cctgacgact gtaaatatta aaagtgacta
     6961 catcggcaac agcagcgcat gcgcgcaccg ccggctcggt cgccggtgaa aaacggcagc
     7021 cgagatagag ctggttcggt gctttttctt tcaaaatatc acgaacagtt gtaaagtatt
     7081 gcaaagcaaa ctgatataaa aagctatctg tatcatcctt gaaagctttc gttctttttg
     7141 acggtctttc aatggcgtcc caggacgaaa agtcgctttc ccaggcatca ttgagagact
     7201 gaatatcttt ataatgctcc tgcagctgtt ttaaaaaagc ttgtcgggcg ggctgctctg
     7261 cattactggc gagcacgccg ccgataactc cttcccagga gagttcattg tcaacaaagt
     7321 agccgataca aagtggattc ccggaatatt ttgccgtcat ggctgaaatc tccggttcga
     7381 tgctttctct gaattccggg tgataggcat cgcatatttt actccaatat ccttctgctg
     7441 cctgaagacg aggtgcattt cctgttgtcg cactggacgt aaaaggaaca gggctggtct
     7501 ccagcataaa aggagggctc cagttgccta tggtggtaaa tccccaggca cgaagccgtt
     7561 tatacgccat ttcccgaccg aatgtcttcc agtctttacc gtattttctg atcatgttag
     7621 ccgcataaaa attgaaggct gagcccgagc ccccgataac atcagccata ctgtgaacgc
     7681 catgcagcac cgtaaaagcc tgagaaaaga taggatcatt tttatcaggc aggtattcaa
     7741 accaggcatc acgctgctcc gcaaaagtat attcactgaa accgacgcaa ttgatcccca
     7801 aagaaaggaa aagtctacct tccggtgtta tgagccacca tttcccgtct atcttttctg
     7861 tgcggaacca gcctgttcct gccagctgcg gacccgccag ccatccaccg aaagaatccc
     7921 gttcggccag ggcaggagta gcttccagca aagttctttc ttcttccagc cgctgtgcga
     7981 aatcaccctc ttctttcagc ttccccggcc agtccccatg cttatattgg ccgaaagcat
     8041 ccacaaaagg catatgcgcg tttatatagt ccgcatgact gccgccgaaa aggaaaacct
     8101 gcttgagggc cagcgtcgta tcttgcgcag gatgagctaa aaaaatttga aaccctgtaa
     8161 tttcctttaa gttcagaggc gccccttttc ccagtggcgc atcaaccggt ggaacgccgc
     8221 gcattcccca gaagaattct ttgtcattgc ggctgaatgc catacaaaca tctttggtct
     8281 ctcctggagc aatctcgctt tcgagattgt tgcaatgatt ggttccatca gcaccgtcat
     8341 tatcaacacg caggcagatg acagcaggag cggcagaagg attgtgtact gtgaagccca
     8401 ttccgaaatg gcctgaaaag tcccaggtat cttcaggtgc atggacgtaa attccgggcc
     8461 agtccgctgg tttaaaaagt atctgcagcc cggattctgt cacttttcct gcatgtgaat
     8521 cgtgaaattg cacagaaggt ggaaggggat gcaataagtc aatcagaacg gttctgttcc
     8581 cgacagtatc cagcggagcg gatgccgcaa aatgactgaa cagtattgcc acaactaagc
     8641 cggggagaaa tgatttcgtc tgcatatatt tcccttgttt gtgtatagta atttaaaaaa
     8701 cctttacaaa aagtaccttt ggggcacaga atcctgcgtt taaaaaaatg cttcagcgct
     8761 tgtccagaat cgcttcggca ggggcggttc cctcaaaatg gtattcatta agaaacaccc
     8821 ctttttcacc gggttccgct gcgttcccaa acaaaatacc gctctgcaac ggcactcctt
     8881 ttgccttcac cgatacctta tccagataca ggttcccgac attaccaaat tccaatgcac
     8941 tgcattttcg cggcagtttc tgctcttcag ggtacacaac agacgcatct tctatcacta
     9001 tattctctgc tccatctgca gcaatcccgg cgccgggcgg ttctgtaatg gcgatgcggt
     9061 caaaacgtat cgcattgact gctctgctga gcggcatcct tccgtccaga gtcctggtca
     9121 gaatctgaat tgcagcacca cgatcaatac taccatacca tcgagagtac cctgtatgct
     9181 caattctaat gtcagaaaaa gcaagacgag ccgggatgac tccttcgggc cactcggggt
     9241 cattgcctat aacgatacca aaaccgccga cattggttat acagatattt tcaacagttc
     9301 catcggtgct cttcagcatc attccatggc gccggtgatt cataaacaca gtgtctttaa
     9361 ttacaaaatc tgctccgcat cgcgagagat tgtatacctg ccagtccacg atacccggac
     9421 gaagcattac caggggatct ttcagtgtca ggcggagtct gccatcttcc tgcctgtcca
     9481 ttctgacaat ggtgctttcc aacagaaggg cgccctgcac aggatcaaca atctgcactt
     9541 tgtcaccttc gcaaaaatac ccgcccgtgc cgatcctgat aacgttgctg tcaatcagtg
     9601 catccataag acaaggacgc gaataaatat tcactccgtc atcagccatt ccctcaaaag
     9661 aacaggcttc gatcagcggc cccgttgtat cctgctgcga gtgaactcca tcactgttag
     9721 tgcttaagaa ccttccggat tcaggtttcg gatatacctt gattttacgg agttgcgtcc
     9781 tgtccgattc aagagaaccg actgccacac tttgtgaggc atagaccgtt acctgcgatg
     9841 caccgcaatg aaaagaccgc caaaaaaata acgcaccgcc attaccgtag cgcgccagct
     9901 gaacaaagcg gtctcccatc tgcatatccg tcagccgatc cttctgagaa tcgacgggat
     9961 acatgcgcca cacacgacgg cttatctgtt tccactgctc cataaagata aagtccgccg
    10021 cacctgcctt gacagttttt gtgtttctgt cgaaaatcat accccattgc ccatgcggtt
    10081 tcggggcatc agaaaaccag gtctcactca aaagcggaaa gttttcctga accgccaaat
    10141 caaaccaccc ttccgaacgt gacaaggcaa gaacggttcc ctgggtaaaa ggaagcggat
    10201 cataatctat ggaaagactt tcaataaaca cattttcgct ttgcccgaca aaaaaagcac
    10261 cctgccgcgg atccgtgaaa atcagttctg tgtcgtctcc ttcgccccga acagtcatgt
    10321 ttctggcttt ttcgatgacc agtgcggaat aatgcctgtc accgccgctg atcctgtaac
    10381 accctttttc cagaagaatc acagacggtc ggcctgaatc aatggcttct ttaataagag
    10441 cccggaatac agggcccata tcttcttccg tatcaggaac cactccgaag tcccgggcag
    10501 aatatagaag cgtgttctct tcctgagcat acagaccgct gaaaggaagc gtgaaaaggg
    10561 cagagaacca taagaggaaa agcattttgg aattcacaag aaaaaccttt tacatccttg
    10621 cagacaacac aggtttacag tatgtctacc agtctatcac agaccgctct tgaagcaaat
    10681 aaggtaaaag cggataatca ggcaagcagg gagaaaatga tgagtacaat aagcagacgc
    10741 cgtttcaatc agtgcctcgc cgccggggcg gggcttgtgc tttcgcagac cctcagcagc
    10801 aggattaagg cagaagaaac cattcgcccg aatatagtct ttcttctggc cgatgatatg
    10861 cggtgggaca ccctgggctg tatgggcaat ccagtgattc aaacacctca tttggatcgg
    10921 ctggcggccg aaggacaggt gtttgatcag tgcagagtta caacgtctat ctgctgctca
    10981 agtcgtgctt ccatactgtc cgggcaatat gtctgccgcc acggcattga tgattttgct
    11041 aaaaccttca ctccggaagc ctttgcccaa tgctacccgg ctctgctgcg aaatcatggt
    11101 tattacacag gttttgtggg caaatacggc gtcggcactg agcttccgga aaaagcattc
    11161 gacttctggc gcggtttcgg cggccagggc aactatgaga atttcgatga agcaggcaca
    11221 ttcaggcatc tgaaccgtat cacagcagag gatagtttgg cttttctgga aaattgcccg
    11281 aaagacagac ccttctgcct gaatgtcagt tttaaagcgc cccattgtca ggacggcgat
    11341 gcacggcaat ttatccatga agaggaatat aatgatttat atgcggattt gatcatgccg
    11401 gtgcccgctt caaacacggt ctcttatgag gacaggttcc ccgagttttt cagagacaac
    11461 aacgaagcac gccgccggtg gaaactccgc ttcgccacgc ctgaaatgca gcaggaaagt
    11521 attcgaaaat actaccgtct gattaccggt ctggacagag tagccggtga gatacgcgat
    11581 aaagtggacg ctctgggctt tggcgaaaat actcttttca tttttacctc agataatggc
    11641 ttttttctgg gggacagcgg tctggcaggg aagtggtacg gctatgaagc gtccatccgg
    11701 gtgcctctta ttatttatga cccccggagc agcagaaaag aacaggacgt acgacattac
    11761 attcccgtac tgaatattga catagctccc actatccttg aacttgctaa tattcctatt
    11821 cctgatacaa tgcaggggaa aagccttgtg tcccggatgc aaggagaaaa atcgatgtgg
    11881 cgtagcgatt ttttctacga acacaacttc catcaccccg gaatcccgaa gtcggaagga
    11941 gtcgtttccc gggaatacaa gtatctgcgg tatcatgagc agaatccttt gtatgaagag
    12001 ttgtttgacc tgaatgcaga tccggatgag gtaattaatc tggcaggcaa agaggaatat
    12061 gaaccgctgc tgaagcgcat gcggaatcgc tttgacgaac tcaaagagga atgctgccgc
    12121 tgatctctct gttaaagctt taaagcagca gatgttctgc gtaaacagga gcgtagctta
    12181 tggtatccct tacggattca gattcagcct cagacaaagt cctgtccgat ttaaaccggc
    12241 tctccggtgc atccagcccg taccttcggc agcatgcgct tcagcccgtt gcatggcagc
    12301 cgtgggacga ggaagcactt ctgaaggcgc agctggaaga taagcccatt tttttgtcta
    12361 tcggctatg
//
LOCUS       DAOPWB010000092        12323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5233_length_12323_cov_6.003505, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000092 DAOPWB010000000
VERSION     DAOPWB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12323
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5233_length_12323_cov_6.003505"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..1187
                     /locus_tag="PKN92_09035"
     CDS             231..1187
                     /locus_tag="PKN92_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ48976.1"
                     /translation="MSKRVVLVTGGAGFIGSHVVDAFIEKGHTVIVLDDLSSGNFDFV
                     NKEAVFVEGDIRSPQIAEIFEQYSPDVICHHASQIDVRKSVDDPAYDADVNIRGSLNL
                     LELAVAHKVKKFIFASTGGAIYGTPAQLPADEQCIPRPECPYGTSKFCVEQYIMLFSR
                     MYNLSYTILRYPNVFGPRQSPEGEAGVCSIFAGLMLDGNTPVLYGKGKAFRDYVYVGD
                     IAAANLLALDKGAGETINLGSEKAITVKELFTHIAHLTEYKGKATLKPLRRGEVEGIY
                     ITGSKAAQVLGWKPEVSLSDGLKKTVDFIRAQREKEKAGDTE"
     gene            1297..2988
                     /locus_tag="PKN92_09040"
     CDS             1297..2988
                     /locus_tag="PKN92_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ48977.1"
                     /translation="MAEYGQLIVLNGAQEGQVVPVCEKVTIGRSESNTLRLTDLQVSR
                     RHAVVLGSGQGVIIKDLDSGNGLYVRGVRVTEQLLEDGDMIRIGKQQIRFEQKQPQEL
                     GSCKAPVEKSAIEAPSSIESKEASVLYKSFFRPPHSSASAIELKGLQKRLQAVYIANQ
                     AIASEQSLDKVFQTVIDQIFSLVKAHNGLILLKQDDKTELSVEFVHNRDPETTVHVSS
                     SIITRVFENGEAILTNSAAEGMGMGGGESIIQQNISSSMCVPLTHQSECLGVIYVDNH
                     GLDNAFNDSDLELLVALAASAASAIKNAQYLKQLEQSYQDTLLALANAIELRDHYTVG
                     HTWRVTNIAMEMARELGWDEEKIKEVQTGGVLHDVGKIAVDNAILCKPAPLTEEEFAQ
                     MKVHPERGADLLRDIKFLKPMIPYCLYHHEKWDGSGYPFGLKEEYIPQEGRLVAVADA
                     FDAMTSTRPYRKAMAPERALNKIIEKKGTQFDPLMVDALEKAYKKGKIEQILQDYHKS
                     EACSIACPFCSTYIRFEESIEAGSQIRCNVCHKTIYLLQQNGRFYGNLTPPNGVE"
     gene            3103..5772
                     /gene="aceE"
                     /locus_tag="PKN92_09045"
     CDS             3103..5772
                     /gene="aceE"
                     /locus_tag="PKN92_09045"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNZ48978.1"
                     /translation="MSTLDKRMLQDWREALEALIDQNGSETALQLLRTLEQDLHTETG
                     VKAFSATTPYVNTIPRDQEPPYPGNRDLERINKSLIRWNAMAMVVQANQASDGIGGHI
                     STYASAATLYEVGFNHFFGAKSDTYSGDQLFIQGHASPGIYARAFLEGRISSELMANY
                     RRELKPGGGLSSYPHPWLMPDFWSYPTVSMGLGPIMAIYQARFNRYLENRGLKPANGG
                     HVWAFLGDGETDEPESLGAITLAARERLDNLIFVINCNLQRLDGPVRGNGQIIQELEG
                     LFRGAGWNVIKVLWGSEWDPLFESDTEGILPQVLGSIVDGEYQKFSVESGAYIRRKML
                     EKDARLAPFLDRLSDTQIRKIQRGGHDPVKVYAAYHRAIHHKGQPTVILAKTIKGYGL
                     GESGEGKNITHQQKKLNEEELWEFRGRFGIPISDEEIAYAPFYRPSEDSPSMKYLKEK
                     RDALGGWLPRRIVNVAPVRKLEDSMFEEFQSGSERPVSTTMVFVRILMKLLRDPDIGK
                     LIVPIVPDEARTFGMDGLFRQFGIYSSIGQLYEPVDAENLLYYKEAKDGQILEEGITE
                     AGAMSSFLAAGTAYASHGINAIPFFVYYSMFGLQRVGDLVWAGGDMRARGFMVGGTSG
                     RTTLAGEGLQHQDGNSHLLAMPVPNLHAYDPAFAYELAVIIQDGIHRMYTLQEDCFYY
                     VTIGNESYAMPPVPQREGIREGILKGMYKFRPAEEVMDTGEAKHKAVLLGSGAIMNEV
                     LKAQEYLSTHYKVAADVWSVTSYKELRNDGMAADRHNMLHPEGPKKKAYVTQCLENEA
                     PVVVAASDYVKMLPDSIARWIPQGITSLGTDGFGRSETRQSLRDFFEVDFRFVTLATL
                     SALMERGELPQETVVNFMKTVGISSNKPNPLFS"
     gene            5815..7143
                     /locus_tag="PKN92_09050"
     CDS             5815..7143
                     /locus_tag="PKN92_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNZ48979.1"
                     /translation="MLTEFKMPELGENVSAGTIARILVNIGDAIAVGQNVLEVETDKA
                     VAEIPSTVAGVVREIRVKEGQKVNTGAVLFTLDSDSAARPAAAAKEEQPVPAQPAAPT
                     AAPAAPKEAPAPATAPKSPAPAAVPSVAQGKVRAAPSTRQFALNNDVDLSLVPSGDGS
                     GRVTTQDVKNYIALRDSGTPSAPAAAAEKEAPAPVSASITAGESGVDTWGAVEVLPLS
                     TLKTKAAVHLTSCWQTIPHVTHFDKADITELEAFRQASARKAEARGVRLTITAFLIKA
                     LPEVLKRFPTFNASIDMDNLQLSLKKYYNIGVAVDTENGLLVPNIRSVDQKNVTDISV
                     EMAELAKKARDRKLTLEEMRGATFTVSNLGGLGGYAFTPIINAPEVAILGVSRSVVEP
                     VYLDGEFQPRLMLPLSLSYDHRAIDGADAARFLRFLCDALAQPWQLLLGA"
     gene            complement(7177..8274)
                     /locus_tag="PKN92_09055"
     CDS             complement(7177..8274)
                     /locus_tag="PKN92_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNZ48980.1"
                     /translation="MKEQFSKLLQRCVQRDASDVHLKVGSPPWLRIDGEMAPVDTEAY
                     SAEMMDIILDIMLNEDQKQYFVKRGEVDVSYTEKGVGRFRVNIFRQRGYISIVMRRIK
                     TTIQTFEQLHLPPAMVTFAEMQRGLVLFTGTTGSGKTTTLASIVDYINRRRKCHIVTI
                     EDPIEYLHQDRKALISQREIAMDTRDFSSALKAMMRQDPDVILVGEMRDLETFQAAIS
                     AAETGHLVFTTLHTTNVVQTIDRIIDLFPSTHHDQIRSQLSLNLRGIMCMRLLPRADG
                     IGRVPTCEVLVVNPAARMIIKENRILQLDTVIASGREAGMQSFNDSLHDLIKSGLITK
                     EVGMDISDNPEDLNMLLQGIRLSSRRGGILK"
     gene            complement(8271..9524)
                     /gene="ftsZ"
                     /locus_tag="PKN92_09060"
     CDS             complement(8271..9524)
                     /gene="ftsZ"
                     /locus_tag="PKN92_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNZ48981.1"
                     /translation="MPDKNTFETFDQTAVIKVCGVGGGGGNAVNRMINARMSDVDFIA
                     INTDAQDLQQSLAPNRLQIGRGNGLGAGAQPEVGRAAAEAHRDEINSMLQGSDMVFLA
                     LGLGGGTGTGAAPVVAQEARKVGALTVAVVTLPFDFEGTLRMKNALEGLKQLEEEVDT
                     LIVVPNDRIAELSDANMSLVEAFRHGDEVLHNGVRAISELITVPGLINLDFADVRTIM
                     GAGGRSLMGIGVATGENRAIRAAAEAINCPLLDQSNIQGAHGVIVNIRGGKDLRIVEV
                     TAANTYIRQNTSPDAQIITGNVVDAEDREEIQITVIASGFPHQDHTEYQSAPASVPEL
                     TSATAPAAPETSSAKEYTHGKTSVAEEMPAAEEVPAAEDESVFALASDEEAEFFQEQP
                     ASTISSVDVNIPPFLQKQYNSRIKP"
     gene            complement(9573..10391)
                     /locus_tag="PKN92_09065"
     CDS             complement(9573..10391)
                     /locus_tag="PKN92_09065"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNZ48982.1"
                     /translation="MAEPKKKSSSAAPKPAPARRWYSRIRIETICGLAALLIIIMIFG
                     YLWIKNSDCDIAQAVKDALAINQILFEGTYMVDEAEIRDAAGITAETTAWNFDQKAAK
                     QRIEKIPYIKHCEVHIRYPWQILIRVTERKAFACVLMHNRLFEIDREGYILRELSGFA
                     LHTGPLITNLPLLNVGVPGTQIESPELMTALSLWDAFVRLPLSDSLTLSELSAESTTL
                     LRMFFEEIPYEIRWGRSDFEKQATRLSILWEDRHGELPCECYLDLRFDADLVCR"
     gene            complement(10378..11847)
                     /gene="murC"
                     /locus_tag="PKN92_09070"
     CDS             complement(10378..11847)
                     /gene="murC"
                     /locus_tag="PKN92_09070"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNZ48983.1"
                     /translation="MQAVNGPRRKVHFIGVGGIGMSGLAEILLNLGYELSGSDVQHSS
                     IVQRLEQFGLTFHRGHAAEHLGDAEIVVVSAAISEDNAEIVAARQRGIPVLHRSELLA
                     DLIRLKPHAVVVAGTHGKTTTTSMISAFLDSANIGATTIVGGILHRSGTNARWGAGDY
                     LVAEADEHDGSFLKLHPTVAVVTSIDAEHLEYYGTLDRIKRAFVDFCNRVPFYGQSIL
                     CGDDLNCRDILPEIEGVTLTYGLNEDAALQGVNIRKGSVAEKKFGDGGMSPFRTLFDV
                     ISHDNRFGQEGFLGTVEIQAVGRHNIQNALAACSVGLCLGMRFAAVAAGLRQFDGVRR
                     RLQWRGTAAGVQVIEDYAHHPTEIASTLEAARWLEPKRIVCVFQPHLYSRTKFFCEDF
                     ARILAQADRALVTDIYPSREQPLPGVDAGLIVDAACALGASHVELVRDMHAVPAEIAR
                     FATAGDMVLVLGAGDVNCIAPAILESLEGVEKERGYGRT"
     gene            12118..>12323
                     /locus_tag="PKN92_09075"
     CDS             12118..>12323
                     /locus_tag="PKN92_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48984.1"
                     /translation="MAAIELSFTAWKPYPAPWHFLHTLAAAHKNSPLLKGDGPLKPET
                     SRHSRHFPGIPAPPSVIPAKAGMTP"
BASE COUNT         3068 a         3283 c         3113 g         2859 t
ORIGIN      
        1 aagccaatgc gcttacttcg gattgctcct cgttttccaa tgctcaggtt taagtgtgaa
       61 aaatcacctt tgaacattgt gcacttgatg acaggaataa agtatactgt actcagaatt
      121 ctttggattc ggatttgttc ctgagtaaag aaacgggatc gccgggcagg ttcaccgcct
      181 tcccccggct taacctttcc tgtattgtat aaattagacg gagatagaga atgtcaaaac
      241 gcgttgttct cgttaccggc ggcgctggat ttatcggctc tcatgtcgtc gatgctttta
      301 ttgaaaaagg tcatactgtt attgtgttgg atgacctttc ttcgggtaac tttgattttg
      361 tgaataaaga agcggtattt gtggaaggtg atattcgttc tccgcagatt gccgaaatat
      421 ttgagcaata cagtcctgac gtaatctgtc atcatgcttc acagattgat gtgcgcaaga
      481 gcgtagacga tcctgcatac gatgccgatg tcaatatccg ggggtctttg aatcttctgg
      541 aactggctgt ggcgcataag gttaagaagt ttatctttgc ctctacgggc ggtgccatct
      601 acggaacccc cgcccagctg ccggcggacg agcagtgcat tcccagacct gagtgccctt
      661 acggcaccag caagttctgt gtggaacagt acatcatgct gttttccagg atgtacaatc
      721 tttcctatac aattctccgc taccccaacg tcttcggtcc ccgtcagagt ccggaaggcg
      781 aggcaggtgt ctgctcaatt tttgccgggc ttatgctcga cggaaacacg cccgtcttat
      841 atggaaaagg taaggcgttt cgtgattatg tgtatgtggg cgatattgcc gctgccaatc
      901 ttctggccct ggacaaaggg gcaggcgaga cgatcaatct gggttcggaa aaagcgatta
      961 ccgtcaaaga actgtttacg catatagccc atctaacaga atacaaaggg aaagccactt
     1021 taaaaccttt gcgtcgcggc gaagtggaag gaatttatat taccggatcc aaagcggcac
     1081 aagtgctggg ctggaaaccg gaagtgtctc tcagtgatgg cttgaaaaaa acagtggact
     1141 tcatccgcgc gcagagggag aaagaaaaag caggagatac tgaataagtg ctcaggatta
     1201 atggtcatta ttttacggtt taaaatagga agctaagcag gaaacggcag agttctaaag
     1261 actggagaag aagtgttctg aataggggat gcgttcatgg ccgaatatgg acaattgata
     1321 gtgcttaacg gcgcacagga aggtcaggtc gttccagtat gtgaaaaggt caccatcggg
     1381 cgaagcgagt ccaatacgct tcgccttaca gacctgcagg tttcccgtcg acatgcggtt
     1441 gtgctcggca gcggtcaggg cgtaatcatc aaagaccttg acagcggcaa cggtctttat
     1501 gtccgcggcg ttcgtgttac ggaacagctg cttgaagatg gtgatatgat ccgcatcggc
     1561 aaacagcaga tccgctttga gcagaagcag ccgcaggaat tgggaagttg caaggctcct
     1621 gtagaaaaat ccgctattga agcacccagc agcatcgaat ccaaagaagc aagtgttctt
     1681 tataagtcct ttttccgccc cccccattcc tctgcttccg ccattgaatt gaaaggtctc
     1741 cagaaacggc tgcaggctgt ctatatcgcc aatcaggcaa ttgccagcga gcagtctctc
     1801 gataaagtgt ttcaaacagt aattgaccag attttttctc tggtgaaagc acataatggc
     1861 ctgatcctgc tgaaacagga cgacaaaaca gaactttccg tggaatttgt gcacaaccgg
     1921 gatccggaaa ctacggttca cgtcagttcc agtatcatta cccgggtctt tgagaatggc
     1981 gaagcgattc tgaccaattc tgccgctgaa gggatgggca tgggcggcgg tgaaagcatc
     2041 atccagcaga acatttcttc aagcatgtgc gtccctctga cccatcagtc agagtgcctg
     2101 ggcgtaattt atgttgacaa ccacggtctg gacaatgcat tcaatgactc cgacctggaa
     2161 ctgctcgtag cccttgcagc gtccgccgca tcagccatca aaaacgccca gtatctgaaa
     2221 caactggagc aatcctatca ggacacgctg cttgctctgg cgaatgccat tgaattgcgg
     2281 gatcattaca cggtcggtca cacctggcgc gtgaccaata ttgccatgga aatggcgcgt
     2341 gaactcggat gggacgaaga aaagataaaa gaagttcaga caggcggtgt tcttcatgac
     2401 gttggaaaaa tcgccgtaga taacgccatt ttatgcaaac ccgctccgct gaccgaagaa
     2461 gaatttgcgc agatgaaagt gcacccggaa cggggagccg accttctgcg cgatataaaa
     2521 tttttgaagc ccatgatccc gtactgcctc tatcaccacg aaaaatggga cggatccgga
     2581 taccccttcg gtctcaaaga agagtacatt cctcaggaag gcaggcttgt ggcagtggca
     2641 gatgcctttg atgccatgac aagcacccga ccataccgaa aagcaatggc accggaacgc
     2701 gccctcaata aaatcattga aaaaaaaggc actcagtttg atccgctcat ggtggacgca
     2761 ttggaaaagg cgtataaaaa agggaaaata gaacagatcc ttcaggacta ccataaaagc
     2821 gaggcatgca gtattgcgtg ccccttctgc agcacctata tccgatttga ggaaagcatc
     2881 gaagccgggt cgcagattcg atgcaatgtc tgccacaaaa ccatttatct gcttcagcaa
     2941 aacggacgct tttatggaaa tttgacgcct cccaatggtg ttgagtaaag aaacaggtgt
     3001 tgtcaaacca attacacctg cctgacgcga atacgctgtt gcagtgttcg caagcccgcg
     3061 cccctgaagt gtatattttc aacgaagtat aaagggaaat ttatgagtac tttggataag
     3121 cgcatgttgc aggactggcg cgaagcgctg gaagcgctga tagaccaaaa cgggtcggaa
     3181 acagcactgc agctgctgcg cactctggag caggatttac acacagaaac cggggtaaag
     3241 gccttttccg ccactacgcc ctatgtcaat accattcccc gggatcagga gccgccttat
     3301 ccgggcaatc gcgatctgga gcgtattaac aaatcgctca tccgctggaa tgccatggca
     3361 atggtggtac aggccaatca ggcttctgac ggcattggcg gacatatctc cacatatgca
     3421 tcagcagcaa ctttatacga ggttggcttt aaccatttct tcggcgccaa atccgacacc
     3481 tacagcggag accagttgtt tattcaagga cacgcctcgc ccggcatcta tgcccgcgct
     3541 tttcttgagg gccgcatttc ttctgaactt atggcaaatt atcgtcgaga gctgaagccg
     3601 ggcggtgggc tctcctctta tccccatccc tggctgatgc cggatttctg gagttatccc
     3661 accgtaagta tgggtcttgg cccgatcatg gcaatttatc aggcacgctt taaccggtat
     3721 ctggaaaatc gcggactgaa acccgccaat ggaggacacg tctgggcatt cctgggtgac
     3781 ggagaaacag acgaacctga atcactgggt gccataacgc tggctgcccg cgagcggctg
     3841 gacaatctta tctttgtgat caactgcaat cttcagcgcc tggatggacc ggtccgcggg
     3901 aacggccaga ttatccagga actcgaaggc ctattccggg gggcaggctg gaatgtgatt
     3961 aaagtactct ggggcagtga gtgggatcct ctctttgaat cggatacaga gggtattctc
     4021 ccacaagtgc tgggctccat tgtagacggc gaatatcaga aattcagtgt ggaatcaggc
     4081 gcctatatcc gcaggaaaat gctcgaaaaa gatgcgcgac tggcaccgtt tctggatagg
     4141 ctcagcgaca cgcaaatccg gaagatccag cgcggagggc atgatcccgt aaaagtatat
     4201 gcggcgtatc atcgtgccat ccaccacaaa gggcagccta cggtgattct tgccaaaaca
     4261 atcaaaggct acggccttgg tgaaagcgga gaaggcaaaa acataacgca tcagcagaaa
     4321 aaactgaatg aagaggaact gtgggaattc agaggacgct tcggcatacc gatttctgat
     4381 gaagaaattg cctatgcacc tttctatcgt ccctctgaag acagccccag tatgaagtat
     4441 ctcaaagaaa aaagagatgc cctcggcggc tggcttccca ggcggatcgt caatgtcgca
     4501 ccggtaagaa aactcgaaga cagcatgttc gaagaatttc aaagcggatc ggaacgccct
     4561 gtctccacaa ctatggtttt tgtccgcatc ctgatgaaac tgcttcgcga ccctgatatc
     4621 ggcaaactga ttgtgcccat tgtgcccgac gaagcgcgca ccttcggcat ggacggtctt
     4681 ttcaggcagt tcggtattta ctcctccatc ggacagctat acgagcctgt ggatgcagaa
     4741 aaccttctgt attacaaaga agcgaaagac gggcagattc tggaagaagg aattactgaa
     4801 gcaggggcga tgagctcttt tcttgctgcc ggaacagcct atgccagcca cggcatcaac
     4861 gccattcctt tctttgttta ttattccatg ttcgggctgc agcgggtcgg agatcttgtc
     4921 tgggccggcg gagacatgcg tgcccgcggt ttcatggtcg gcggcacctc cggaagaacc
     4981 acacttgccg gagaaggatt gcagcatcag gatggaaaca gccatctcct tgccatgccc
     5041 gtacccaatc tgcacgccta tgatccggca tttgcttacg aactcgctgt gatcattcag
     5101 gacgggatcc atcgcatgta caccttgcag gaagactgct tttattatgt gacgatcggt
     5161 aatgaaagct atgccatgcc gccagtgccc caacgggaag gtatccgcga agggatcctg
     5221 aaggggatgt acaaattcag gcctgccgaa gaagtcatgg acaccggcga ggcaaagcac
     5281 aaagccgtac tccttggcag tggtgctatt atgaatgaag tgctgaaagc ccaggaatat
     5341 ctttccaccc attataaggt tgctgcggat gtctggagtg ttaccagcta caaggaactt
     5401 agaaacgacg gcatggcagc agatcgtcat aacatgctgc atcccgaagg cccgaaaaag
     5461 aaagcctacg tcactcaatg tctggaaaat gaagctccgg tggttgttgc cgcttcagat
     5521 tatgtaaaaa tgcttcccga cagtatcgca cgctggatcc cgcaggggat caccagtctg
     5581 ggcacagatg gcttcggcag aagtgaaaca aggcagtctc tgcgtgactt tttcgaagtt
     5641 gacttccgtt ttgttacact tgcaaccttg agtgccttga tggaacgggg tgaacttcca
     5701 caggaaaccg tggtgaactt catgaaaacc gtcgggatct cctctaacaa gccgaaccct
     5761 ctgttcagtt aaacttttgt ggcgtattcc acttgaaata gaagggaaat ttgcatgcta
     5821 actgaattta agatgccgga actgggtgag aacgtcagtg ccggaaccat tgcccgtatt
     5881 ttagtcaaca taggcgatgc gattgccgtc gggcagaatg tattggaagt ggagacggat
     5941 aaggccgttg ccgaaattcc aagcaccgtg gcaggagtcg tccgggaaat ccgtgtaaag
     6001 gaaggacaga aagtgaatac cggcgctgtg ctttttacac tggacagcga cagcgccgcc
     6061 cggccggcag cggctgcaaa agaagaacag cccgttccgg cacaaccggc tgcaccgacg
     6121 gcagcgccgg cagcaccaaa agaagcgccg gccccggcca ctgcgccgaa gtcgcccgcc
     6181 ccggcagccg taccctctgt ggcgcaggga aaagtgcgtg cagcaccgtc gacgcgacag
     6241 ttcgctctca ataatgacgt ggatttatcg ctcgttcctt ccggcgacgg ctccggcaga
     6301 gtgaccactc aggatgtaaa aaactacatt gccctgcgtg acagcggaac tccgtccgct
     6361 cctgctgcag ctgctgaaaa ggaggctcct gctccggtat ctgcttctat tactgcgggc
     6421 gaaagcggcg tcgatacatg gggcgccgtt gaagtgcttc ctcttagcac tttgaaaaca
     6481 aaagccgccg tgcaccttac ctcatgctgg cagaccatcc ctcatgtaac ccattttgat
     6541 aaagcggaca ttactgagct cgaagctttc agacaggcat ctgcacgcaa ggcggaagcc
     6601 cggggcgttc gtcttaccat taccgctttt cttatcaaag cattgccgga agtactgaaa
     6661 cgtttcccga ccttcaatgc atccatcgac atggacaatc tgcagctctc gctgaaaaaa
     6721 tattacaata tcggcgtggc agtggacact gaaaacggtc tgcttgtacc caatatccgg
     6781 tctgtagatc agaaaaatgt gacggatatt tccgttgaaa tggctgaact ggcgaaaaaa
     6841 gcacgggaca ggaaactgac tctggaagaa atgcggggcg ccacctttac tgtgagcaat
     6901 ctcggcggcc tcggcggtta tgcattcaca cccattatta acgctccgga agttgctatt
     6961 ctcggcgttt cccgttcagt ggttgagccg gtctatctgg acggtgaatt ccagccccgg
     7021 ctcatgctgc ccctcagtct gtcctatgac catcgtgcaa tcgacggcgc ggatgcggcg
     7081 cgttttctgc gcttcctctg tgatgcactg gcacagccct ggcagctgct gctgggtgca
     7141 taagcagcaa aggtctttgt tccctccgga ggcggcttat ttcagaatgc cgccccggcg
     7201 ggagctcagc cgaatcccct gaagcagcat gttcaggtct tccggattgt cggaaatatc
     7261 catgcctacc tctttagtaa tcaggccgga tttaatcaga tcatgcaggc tgtcattgaa
     7321 gctttgcatg cctgcttccc ttccgctggc gatgacggta tccagctgga gtatgcggtt
     7381 ttccttgatg atcatccttg ctgcaggatt aaccaccagc acctcgcagg taggcacacg
     7441 gccgattccg tccgctctgg gcagcaggcg catacacatg atcccgcgca aattaagcga
     7501 caactgtgag cgtatctgat catgatgtgt tgaagggaaa agatcaataa tgcggtcgat
     7561 ggtctgcacc acattggttg tgtgcagtgt ggtgaaaacc agatgccctg tttccgccgc
     7621 actgattgcg gcctgaaaag tttccagatc acgcatctcg cccacaagaa tcacatccgg
     7681 atcctggcgc atcatcgctt ttaatgccga cgagaaatct ctcgtgtcca tcgcaatttc
     7741 gcgctggctt atcagtgctt ttctgtcctg atgcagatat tcaatgggat cttcgatcgt
     7801 gacaatatgg catttccggc gtcggttaat gtaatccacg atggaggcaa gggtggttgt
     7861 ttttccgctg cccgtcgtac cggtaaacaa caccagcccg cgctgcattt ctgcaaaagt
     7921 gaccatagca ggcggcaggt gcagctgctc aaaagtctga atagtggtct tgatgcgccg
     7981 cataacaatg gaaatataac cgcgctggcg gaaaatattg acacggaagc gacccacccc
     8041 tttttctgta taagacacat ccacttcacc ccgcttgaca aaatactgct tctgatcctc
     8101 attcagcata atgtccagta taatgtccat catttctgcg ctataagctt ctgtgtccac
     8161 cggtgccatt tcgccatcaa tgcgaagcca gggagggctg ccgactttca gatgtacgtc
     8221 tgaggcatcc cgctggacac atcgctgcag taattttgaa aactgctctt tcatggcttg
     8281 atcctgctgt tatattgttt ttgcaagaaa ggagggatat tcacatcgac actgctgatc
     8341 gtggaagccg gctgctcctg aaaaaattct gcttcttcat cagatgccag ggcaaaaaca
     8401 ctttcatctt cagcagccgg aacttcttct gcagctggca tttcttcagc aacagacgtt
     8461 tttccgtgcg tatactcctt tgctgacgac gtctcaggcg ccgccggggc tgttgcggaa
     8521 gtgagttccg gaacagatgc gggtgcagac tgatactccg tgtggtcctg atgcggaaat
     8581 cctgacgcaa tcactgttat ctgaatctct tcccggtctt ccgcatccac cacattaccc
     8641 gtaatgattt gcgcatccgg actcgtattc tgcctgatat acgtatttgc ggcggtaact
     8701 tcaacgatcc gcagatcttt gccgccccgt atattcacga taacaccgtg ggctccctgg
     8761 atattggact gatccagcag agggcaattg atagcctctg cagcagctct gatggcgcga
     8821 ttctcgccgg tggcgacacc gatacccata agggagcgtc ctcccgcacc cataatcgtg
     8881 cgaacgtccg caaagtcaag attgatcaga cctggcacag taatcagttc agatattgca
     8941 cgcacgccgt tatggagcac ctcgtcaccg tggcggaatg cctctacaag agacatgttg
     9001 gcatcgctca gctcggcgat gcggtcattg ggaacgacaa tgagcgtgtc cacttcttct
     9061 tccagctgtt tcagaccttc aagcgcgttt ttcatacgca gcgttccctc aaaatcaaag
     9121 ggaagggtga ccacggcaac cgtcagcgca cccactttcc gtgcctcctg tgccaccacc
     9181 ggtgcagctc ctgttcctgt cccaccgccc aggcccagtg ccagaaaaac catatccgag
     9241 ccctgcagca tgctgttaat ctcatcgcgg tgtgcttccg ccgctgcccg gccgacctca
     9301 ggctgcgcac cggcgcccaa gccgttcccc ctcccgatct gcagcctgtt cggtgccaga
     9361 gactgctgta aatcctgtgc atccgtgttg atcgcaatga aatcaacgtc ggacatgcgg
     9421 gcattgatca tccggtttac cgcattgcct cctccgccgc ctacaccgca cactttaatg
     9481 acagccgtct gatcaaaagt ttcaaatgtg tttttgtctg gcatcgtctt ttctccttct
     9541 gaaagacatc atttaccgac ccgtatccgc cactatctgc aaaccagatc tgcatcaaaa
     9601 cgcaaatcca gataacactc gcagggaagc tcgccgtggc ggtcttccca gagaatactc
     9661 aggcgtgtcg cctgcttttc gaaatcggag cgtccccaac ggatttcata aggaatctcc
     9721 tcaaaaaaca tacgcaaaag agtggtgctt tcggcagaca actcggaaag cgtaagactg
     9781 tccgacaagg gaagccgcac aaaagcgtcc cacaatgata gcgccgtcat aagttccggg
     9841 ctttcaatct gcgtgccagg cactcctaca ttcaacagag gcaggttggt gatcagagga
     9901 ccggtatgca gagcaaaccc ggacagttcg cgcagaatat agccctcccg gtcaatctca
     9961 aaaagacggt tgtgcataag cacgcaggcg aaggccttgc gctctgttac ccggatcaga
    10021 atttgccacg ggtacctgat gtgcacttcg caatgtttga tatagggaat tttctcgatt
    10081 ctctgttttg ccgccttctg atcaaaattc catgctgtag tttcggcggt aatccctgca
    10141 gcgtcccgaa tctccgcctc gtccaccatg taagtgcctt caaacaaaat ctgatttatc
    10201 gctaaagcat ctttgacagc ctgggcaata tcgcaatctg aattcttgat ccataggtac
    10261 ccgaagatca tgataattat aagcagtgct gcaagtccgc atattgtttc tatacgaatg
    10321 cgagagtacc agcgccgggc tggcgccggt tttggagcag cagatgaaga ttttttctta
    10381 ggttctgcca tatcccctct ctttctccac tccttccaga ctttcaagaa tggctggggc
    10441 aatgcaatta acatcgcctg cacccagcac cagcaccata tcgccggcag ttgcaaaacg
    10501 tgctatttct gccggaactg catgcatgtc gcgcacaagc tccacgtgag aagcgcccag
    10561 tgcacatgcc gcatccacaa tcagccccgc gtccacgccg ggcagtggct gttcgcgaga
    10621 tgggtaaata tctgtcacca gcgcccgatc cgcctgagcc agaatcctcg caaagtcttc
    10681 acagaaaaat ttagtgcgac tgtaaaggtg aggctgaaac acgcatacaa tccgcttcgg
    10741 ttccagccag cgggctgctt ccagagtgct cgcgatttcg gtcgggtggt gtgcgtaatc
    10801 ttcaatcact tgcactccgg cagctgtacc gcgccactgc aggcggcggc gcaccccgtc
    10861 gaactggcga agacctgctg ctaccgcagc aaagcgcatg cccagacaca aaccaacaga
    10921 gcatgccgcc aacgcattct gtatgttgtg acggcccacc gcctgaatct ccaccgtacc
    10981 gagaaatccc tcctggccga aacggttgtc atggctgatg acatcaaaaa gcgtacgaaa
    11041 ggggctcatg ccaccgtcac caaatttctt ttccgcaacg gaacctttgc ggatattgac
    11101 cccctgaagt gcggcatctt cgttaaggcc gtaggtgagc gtgactccct cgatttcggg
    11161 caggatatcc cgacagttca gatcgtcgcc acacagaatg gactggccgt aaaaaggaac
    11221 tctattgcag aagtccacaa aagcccgttt gatccggtca agggttccat aatattcaag
    11281 atgctcggca tcgatgctgg tcactaccgc aacagtcgga tggagcttca gaaaagagcc
    11341 gtcatgttcg tccgcttccg ctaccaggta atccccggcg ccccaccgtg cattggtgcc
    11401 gctccggtgc agaataccgc cgacaatggt tgtggcgccg atattggcgc tgtccagaaa
    11461 tgcgctgatc atggacgtgg tggtcgtttt gccgtgcgta ccggcgacaa ctactgcatg
    11521 gggcttcagc cggataaggt cagcaagcag ttcgctgcgg tgcagtaccg gaataccgcg
    11581 ctgccgtgcc gccacgatct ccgcattatc ttcggaaatg gcggcagaca cgactacaat
    11641 ctccgcatca cccagatgct cggcggcatg tcccctgtga aacgtaagac caaattgctc
    11701 cagccgctgg acaatggaag aatgctgtac gtccgatccc gacagctcat accccaaatt
    11761 cagcagaatc tcggcaagac cgctcatgcc gattccgcca acaccgataa agtgcacttt
    11821 tctccgggga ccattaacag cttgcatgac tctttatccc atatattgtg gtttttctat
    11881 tataccacaa gataaaccac aataaaaagg ggcaatttag tgaacaaatg tacctcgcag
    11941 cccgcttggg ctaagaagca tgtcttcaga gcgcatataa taattccaaa aaatacggga
    12001 gtgacggtgc ttgattgtat ccggggggca ggctgtggat aatacaagca agaaaaaaaa
    12061 tatcaagcag atcaggcgtg caaaaattaa atcgcctctt tagcagagca caaaaaaatg
    12121 gcagccattg aactttcctt tacggcatgg aaaccgtatc ccgcaccctg gcattttctt
    12181 catacgcttg ccgctgccca taagaactct ccattgctga aaggcgacgg cccattgaaa
    12241 ccggaaactt cccggcattc ccgccacttc cccggcattc ctgctcctcc ctccgtcatt
    12301 cctgcgaagg caggaatgac gcc
//
LOCUS       DAOPWB010000093        12248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5277_length_12248_cov_5.446322, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000093 DAOPWB010000000
VERSION     DAOPWB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12248
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5277_length_12248_cov_5.446322"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..671)
                     /locus_tag="PKN92_09080"
     CDS             complement(198..671)
                     /locus_tag="PKN92_09080"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ48985.1"
                     /translation="MSNAYSIVFLFCTAACILGIQLGMQFWRNQRARNRFLQGIHFME
                     QQNYDAALKAFRKTCGLTPQSLQPRIFLATALVHTGNEKEALEHIALLEELHPNEAQA
                     WALICIFYITNLPRCRDACSAAFERVKALDAGVADEILSHSQFRRYLQTEGRQES"
     gene            884..1201
                     /locus_tag="PKN92_09085"
     CDS             884..1201
                     /locus_tag="PKN92_09085"
                     /inference="COORDINATES: protein motif:HMM:NF026214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNZ48986.1"
                     /translation="MSEVKAFFDGLAGRVNADKIAGMNAVFQFNLDDEVFVVAIADNA
                     VQVSEGAVEKPSIQLTMTSADFLDLVSGKLNGQTAFLTGKLKVKGDMTLALKLQSVFN
                     IGG"
     gene            1330..1905
                     /locus_tag="PKN92_09090"
     CDS             1330..1905
                     /locus_tag="PKN92_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48987.1"
                     /translation="MTLYRSRTDLSPALLLLPGKYRSAAVWEGFARRLQHEGFALLVL
                     DDRDLTGQPLEELYRRLDAAKDRLVESGAHPLNLAVAGESTAAGLALLYAAHCPDMQA
                     AVLLSPPLAYLDQNLESVMAVLKDCPSLIAAAEGDRYGAQAATVLKKSAPVFAELRLW
                     PGSAHGTDLFAGHPEAVMQIVQWLKTVLNAS"
     gene            complement(1920..4193)
                     /gene="nrdD"
                     /locus_tag="PKN92_09095"
     CDS             complement(1920..4193)
                     /gene="nrdD"
                     /locus_tag="PKN92_09095"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNZ48988.1"
                     /translation="MSDDFQYTLPFPPEPENEGLFDPQMGLPLSSDNWLQPIREIVKR
                     NGKKEIFNKQKISDAIFNASRQAGIEDRNLADSLASAVTLYISKQARGKAPTVEQVSD
                     AVETVLIQLSQPQAALAYVRYRDRRARIRRLRQGDMKGLLGELEEARLERESANAIAN
                     SLQVQTSQENYACWDRQKIVTALCLETGLQADLAELIAAEVETQIQNAGLNVLTTPLI
                     RELVGAKLLEHGLVEENEQRRRLGVPLYDASRLIRGIAPETLGASPAWTDQALARAVK
                     KEYALSEVFSSEVTSAHFNGSLFLEGLDHVDRLCSADVSIGSLMKQEKKSALEFHPAM
                     IVARMIQFTLQLDAFFMEAPCWHAFNHQAAPLLEKSSDRDLAAFSRAVLEEFDFRAHQ
                     GRNTLSPETLRLHWSAPCIPDTWLQPGMAGSDLDDPYDAWESVARRLALKLLEIFEEG
                     RHHGPYAAPQLEIILEPALFRHFEGIAFLRQAVRTSLKQPGIRFLLPHRSEDDAPAPA
                     GCCENSIVSALWNRIILNTPGIALMGETEAAFFNRLESYFRLAVQAHREKRDFVEGLM
                     GGVHQGPLSFPAAAGGACFPETGRFVIAVDGLYECAEIMAGSRPFSHRERLTFMEKTL
                     TFLGQLTEESSKKEDMDLLLGANSNPEISKRFANLDISRHGNALKEVVKILPDTQECS
                     YTPGVGFPESSGLTPFDKAQYEGTLHKVLKTPSITSLKLPVRHASEYTLTDLLKKIMY
                     QTDCRGVQLSMEEKRDA"
     gene            complement(4222..6360)
                     /locus_tag="PKN92_09100"
     CDS             complement(4222..6360)
                     /locus_tag="PKN92_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48989.1"
                     /translation="MPPHQLQKALGQFMVQTFLAFLNQISANYPLHFFLPVAAMIIMA
                     CYYLLNRNSVKAWLGEKRLLFLRGFLLAPLFAVAVLAVENYRYTDYYRFDSYLNAYEF
                     YHYYIGTKYAREVGYGDMYMASLVADSETGMKWKHSSGTITDLNTGKKVSAKTVLAQA
                     DKYKSRFTPERWEEFKKDIVWFKDRMIQSRWSGVLRDKGYNGTPVWTMLVGGLLSNRV
                     STDSDRNMMILAVLDPLLILITFLMVLWAFGPRTAFLMITLLGTCYMMKYWHLKGAYL
                     RTDWVMCLVMSVCLIRKKHFALAGALTGYATLSRIFPAVLLFGIGARFFWLILDYCLM
                     LLRRKYLSLGLQKRSLGERLVVKATIFLFIFLLLWGSYAFVSAVALPWIGSPDRSISG
                     FFNQMVSGAGGHSAFLQLFTFISWVALALAGAVTTLRGLWLKEIDRRYFYFFLSFAVI
                     TATLFGVSLIYWHGTDYWAQYTEKIGRHNTDISTWRMGFKYIFMADFGQDFNFLEKAF
                     KDWTPVVRPAWYMEKHLIWWKIQLLVLAVTLAACRNIKDYRAYILGFVPLFFLVSPTY
                     YYYIMLLVPLLFFAPRIDHGRYAVGLALMYITGMVGFGFYGLWRQDYATYYWLSVLVM
                     ITVLYMLFLALLENTTAVMAQKRLRALAEATPTEGATPIKSEGAPCCPDVAPAKEASG
                     DVSAGALSEEILKVVPGAVSDRQSDTPPVE"
     gene            complement(6471..7754)
                     /locus_tag="PKN92_09105"
     CDS             complement(6471..7754)
                     /locus_tag="PKN92_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ48990.1"
                     /translation="MSTSISRRSFMAQAAAGSAALAVASSAGARTVLPGSKIQIGVIG
                     MSRGKALAHTFGASDQCRIRYLCDVDTERLGAAAAGFEKAFGYLPERTGDFRHILDDP
                     EVDAVVMALPVHWHAPLSILACQAGKHVYVEKPCCHNPREGELLVAAARKYGRVVQMG
                     TQRRSSPVIREGIARLHAGEIGAVSYVRSWYASVRGTIGKGVEAAVPASLNYDLWQGP
                     APRKPYKDNVIPYNWHWFWHWGTGELGNNGTHMLDLARWGLQVDFPSRVTARGGRFRF
                     DDDQETPDTLTVSYEFPGEKMVLWEGLSCNRPGIDTDVGVCFFGEKGSLTLRSQDYTL
                     RDPDGKVLDQQSGPLGDEEHRDNFLAAIQDGTPDTLHAEIETAFRSTLLSHLGNIAYR
                     TDSAVHCDGQGHILDNPAAAALWQRDYEPGWAPEG"
     gene            complement(7833..8672)
                     /gene="arsM"
                     /locus_tag="PKN92_09110"
     CDS             complement(7833..8672)
                     /gene="arsM"
                     /locus_tag="PKN92_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNZ48991.1"
                     /translation="MDQDKESQRAHVREGYGRIAQEEGSCCAPKRTCCCGDNADALAK
                     TVGYSDAELALLPEGANMGLSCGNPTAIAALKPGEWVVDLGSGGGFDVFIAGEKVGDD
                     GRVIGVDMTPEMISLARRNIETYRRRTGKDNVEFRLGEIEHLPVADNAADVVISNCVI
                     NLSPDKKQVWQEIFRVLKPGGRAAISDMALLKSLPPDVKEQSTALIGCMAGAVFVEDT
                     EALAKEAGFTDIELTVKSDYIETMTGLKASLYGEIRDHMPEGESLSDYIVSLSVSARK
                     PAH"
     gene            complement(8692..9054)
                     /locus_tag="PKN92_09115"
     CDS             complement(8692..9054)
                     /locus_tag="PKN92_09115"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNZ48992.1"
                     /translation="MKVFMMQAKAIADETRARILLLLAEEELCLCQLIALLDLAPSTV
                     SRHMSILVQADLVLSRKEGRWKFYRLPDKRPDDLAAELLHWVSRSLKSADIVKKDKKQ
                     LVRVLKQDKKELCDRYCC"
     gene            9320..9757
                     /locus_tag="PKN92_09120"
     CDS             9320..9757
                     /locus_tag="PKN92_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48993.1"
                     /translation="MFRKKMMLWIPVAVLLLLTGCNTLSSAPQITKAAITPEVLRPGD
                     TAVITMQIHDRSRVVKRVEGIVLEDARITFKLRDDGAEPDEKANDGIWSMQVDVPFQA
                     PPGEFRLEFTAYGQDGTPIPVRDRDGHISPLQQVIPLRIEYQQ"
     gene            9761..11038
                     /locus_tag="PKN92_09125"
     CDS             9761..11038
                     /locus_tag="PKN92_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNZ48994.1"
                     /translation="MAAFLLWISCLTAAVADPLPDVVLLSVDTLRADVLGCYGSPHPV
                     TPELDRFSETALIFDDAQCEIPLTGPSFSAMFTGRFPRELGVTRNGLKLPEDMRTLTR
                     VFREHGYHCLAVQSNWTLRGKLCGLNRDFDVYDDELPQKRWGVYRGERRAADLLKAAE
                     KHLKERPEGKPLFAWIHFSDPHAPYRFRRDFDPAGTDFRKGDKKELTRARYMSEVNYC
                     DSHIGALLRALPEKARILFVADHGESLHDHGYLGHGRHVYSECTRIPLMIRSPELKPG
                     RSAYPASSLDVGVTLLALAGLPPLPGMRGVNLCRAEALPRRPRFTETYGGLAPGIPGV
                     RQMLQDTQPLRCAVSFEGWKLHVLSSQQMELYHLTEDPGEEKDLRYRYPERIKVLKAL
                     LDDWEATCPAGRAEDASLNRDDLEALRSLGYIE"
     gene            complement(11058..11468)
                     /locus_tag="PKN92_09130"
     CDS             complement(11058..11468)
                     /locus_tag="PKN92_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNZ48995.1"
                     /translation="MYRFSVETRARTQFINIDSQIRKLLTECGLEAGILQLHVPHTTA
                     GITINENADPDVPRDMELVLDRMVPWHDPAYRHAEGNTAAHVKASMTGAGCTLIIEGG
                     RPCLGVWQSVFFCEFDGPRRREVWAVPVPLPTKE"
     gene            complement(11471..>12248)
                     /locus_tag="PKN92_09135"
     CDS             complement(11471..>12248)
                     /locus_tag="PKN92_09135"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48996.1"
                     /translation="ILNRLERFLSFIPEPYRSLTVKDIKLFWRDPTQWSQFVIFFGIM
                     AIYIANLRSSSRYFEDAMWRSWIACLNVGSVTLILSTLTSRFVFPLVSLEGRRFWIIG
                     LAPFRFTGLVWQKFWLSVCVTSLFTVGLSFLSATMLKLEPVFFWMTVYSVVVANFGLA
                     GLAVGLGALYPSFHEDNPARIVSGLGGTLNLLLSIAYITVIIAAQTLVLQWNALGGFA
                     SPALFHYALTAAISVGLLTTLAAVGLPMYFGLRNLRDREY"
BASE COUNT         2805 a         3327 c         3360 g         2756 t
ORIGIN      
        1 gggctgcatc ataaaaagcc gcccactcgg gaagcggttg agagaggtca atcagagtga
       61 ctgtatcctc agcccggcag tctgttgccg cagccgtgaa tgccagacaa aacgctgtga
      121 aaaaaaacaa catgcttcag tctttcgata tgtcggttgt tcggggaaga tatgcagggc
      181 gggaataggt aaaaggatca ggattcctgc ctgccttctg tctgaaggta gcgcctgaac
      241 tgactgtggc tcaggatttc atccgccaca cctgcatcca gggcttttac gcgttcgaaa
      301 gccgcactgc aggcatctct gcagcgggga aggttggtga tgtaaaaaat gcagataagc
      361 gcccatgcct gtgcctcatt ggggtgcagt tcctcaagca atgcaatgtg ttccagtgct
      421 tctttttcat tgcctgtgtg aacgagtgcc gtggcgagaa aaatgcgggg ctgcaggctt
      481 tgcggtgtca gtccgcaggt cttgcggaac gctttcagag cggcatcgta attctgctgt
      541 tccatgaaat gaatgccctg cagaaagcgg ttgcgtgccc gctggtttct ccagaactgc
      601 attcccagct ggatgcccag gatgcacgct gccgtgcaaa aaagaaaaac tatactgtat
      661 gcgttggaca taacggtctt tctcaggaat taatgtaccg ataaaaaata aaaaacagag
      721 acggggcttg cttttttcgc ctgaatttac tatgataact gctaatggat accgattccg
      781 tctgcctttt gcatctatat accgacggtt aagtaagtaa tgggaaaagg cggaaaaggc
      841 atcagagcag cctcaactat aacagaaaat ggaggagtcg atcatgtctg aagtcaaagc
      901 tttttttgac gggctcgccg gtcgtgtgaa tgcggataaa atcgccggaa tgaatgccgt
      961 gttccagttt aatctggatg atgaagtctt tgtggtcgct attgcagata atgcggtgca
     1021 ggtaagtgaa ggcgccgtgg aaaaaccctc catccagttg acaatgacat cggctgattt
     1081 tctggatctg gtcagcggta aactgaacgg gcagaccgct tttcttacag gaaaactgaa
     1141 agtcaaaggc gacatgacgc tggcactgaa actgcagagc gtgtttaata tcggcggatg
     1201 aaataccgct gttgctgtct ttccctgatc ctttgcctgc tgtctgtttc ctgccatcga
     1261 caggatgcgc ctgtagaggc aaaggaggag agactgatat tgccttccgg gagcggggaa
     1321 gccctgtctg tgactttata ccgctcccgg acagaccttt cgcctgccct tttgcttctt
     1381 cccgggaagt atcgttcggc ggcggtgtgg gaagggtttg cccggcgcct gcagcatgaa
     1441 ggctttgcgc tgcttgttct cgacgaccgg gacttgaccg ggcagcctct ggaagagctg
     1501 tacaggcgcc ttgatgcggc aaaagaccgg ctggttgaat caggcgccca tcccctgaat
     1561 cttgccgttg ccggtgagag cactgcggcg gggctggcgc tgctctatgc ggcgcactgt
     1621 cccgacatgc aggccgctgt gctgctgtcg ccgccgctgg catatctgga tcagaatctg
     1681 gagtctgtta tggcagtgct gaaggactgt ccctccctga ttgccgctgc ggagggggat
     1741 cgctatggcg cccaggcggc aactgttctg aaaaaaagcg cacctgtttt tgccgaactt
     1801 cgattatggc cgggctctgc tcacggcacg gatctttttg ccggacaccc cgaagccgtg
     1861 atgcagatcg tgcaatggct taaaacggta ctgaacgcct cctgagggag ggtatcgcct
     1921 tatgcgtccc gcttctcttc catggaaagc tgcacacccc ggcaatctgt ctggtacatg
     1981 atttttttca gcagatctgt cagtgtatat tcggaggcgt ggcgcacagg aagtttcagc
     2041 gaagtgatag aaggggtttt aaggacttta tggagtgtcc cttcgtattg tgctttgtca
     2101 aagggggtaa gcccgctgga ttccgggaaa ccgactcccg gcgtataact gcactcctgg
     2161 gtatcgggca gaattttaac gacttctttc aacgcattgc catgcctgct tatatcgagg
     2221 ttggcgaatc gcttgctgat ctcgggattg ctgtttgctc cgagaagaag atccatatcc
     2281 tcttttttcg agctttcttc tgtaagctgc cccagaaaag tcagtgtttt ttccatgaat
     2341 gtgagacgct cccgatgtga aaacggtcgg cttcctgcca tgatttcggc gcattcgtaa
     2401 agcccgtcca cagcaatgac aaagcgtccg gtttcaggaa aacaggcgcc tccggcagcg
     2461 gcagggaagg aaagaggacc ctgatgcacg ccgcccatga gcccttccac aaagtcccgt
     2521 ttttcacgat gcgcctgtac cgccagcctg aaataggatt cgagtctgtt gaaaaacgcg
     2581 gcttccgtct cgcccatcag agcaatgccg ggcgtgttca gaataatccg gttccacaga
     2641 gcgctcacaa tactattttc gcagcagccg gcgggcgcgg gggcatcgtc ttcgcttcgg
     2701 tggggcagaa gaaaacggat accgggttgt ttgagcgaag tgcgtaccgc ctgcctgaga
     2761 aatgcaatgc cctcaaagtg ccggaacagc gccggttcca gaatgatttc cagctgcggt
     2821 gccgcatagg gaccgtgatg ccgtccttct tcaaagattt ccagcagttt taacgccagg
     2881 cgccgcgcca ccgattccca ggcatcatag ggatcgtcga gatcggaacc cgccatgccg
     2941 ggctgcagcc aggtgtctgg aatacagggg gcgctccagt gcagccggag tgtttccggt
     3001 gagagcgtat tgcgcccctg atgtgcccgg aaatcaaatt cttccagcac cgctctggaa
     3061 aaagcggcaa gatcccggtc gctgcttttt tcgagcagag gcgctgcctg atgattgaaa
     3121 gcgtgccagc agggcgcctc catgaaaaaa gcgtcaagct gcaaagtgaa ctggatcatc
     3181 cgcgccacta tcatggcagg atgaaattcc agtgctgatt ttttttcctg cttcatcagc
     3241 gagcctatgc tgacatcggc ggagcagagg cggtcaacgt ggtcaagccc ttccagaaaa
     3301 aggctgccgt tgaaatgggc ggaagtcact tcggacgaga agacttcaga cagggcatac
     3361 tcttttttta ctgcccgggc aagcgcctgg tctgtccagg cgggggaggc gcccagtgtt
     3421 tccggagcga tgccgcggat cagacgggac gcatcataaa gcggaacacc cagacggcga
     3481 cgttgctcgt tttcttccac aagcccgtgt tccagcagtt ttgccccgac cagttcccgg
     3541 ataagcgggg tcgtcagcac attcaggccg gcattttgaa tctgcgtttc cacttccgca
     3601 gcgattaatt cggcaaggtc ggcctgaagc cctgtttcaa gacacaaggc tgtcacgatt
     3661 ttctggcggt cccagcaggc ataattttcc tgactggttt gtacctgcag gctgttcgca
     3721 atggcatttg cactttcccg ctccaggcgt gcttcttcca attcgccgag aagccctttc
     3781 atatctccct ggcgcaggcg tcggattcgt gcccggcgat cccgatagcg tacataagcc
     3841 agggccgcct gaggctggga cagctgaatc agcaccgttt ccaccgcatc actgacctgt
     3901 tccactgtgg gcgctttacc ccgtgcctgt ttgctgatat aaagcgtaac ggcggaagcg
     3961 aggctgtccg caagattcct gtcttcaatg cctgcctggc gggacgcatt gaagatggca
     4021 tcggaaattt tctgtttgtt gaaaatctct ttctttccgt tgcgctttac aatttcccgg
     4081 atcggctgca gccagttgtc agaagacagc ggaagcccca tctgaggatc gaaaaggccc
     4141 tcattttcag gctccggcgg aaaaggaagt gtatattgaa agtcgtcgga cacaggagtt
     4201 cctcagctat aaaaaggaat actactcaac aggcggtgtg tcggactgcc gatctgaaac
     4261 agctccgggc accactttca aaatttcttc ggatagcgct ccggcagata cgtccccgga
     4321 ggcttctttg gcgggtgcca catccgggca gcacggagca ccttcggatt ttatcggtgt
     4381 ggcaccttcc gttggggtgg cttctgccag cgcacgcagc cgcttttgtg ccataacggc
     4441 tgtggtattt tcaagcagcg caagaaagag catgtagagc acggtaatca tgactagcac
     4501 ggacagccag taataggtcg catagtcctg ccgccacaga ccatagaaac cgaagcccac
     4561 catgcctgta atatacatca gcgccagacc gacggcataa cggccgtgat caatgcgggg
     4621 cgcaaaaaac agaagcggta ccagcagcat gatgtaatag taataggtgg gtgataccag
     4681 gaaaaagagg ggtacgaagc ccagaatata ggcgcggtaa tctttgatat ttcgacaggc
     4741 ggcgagtgtg acggcaagca ccagcagctg aatcttccac cagatcaggt gcttttccat
     4801 ataccacgcc ggtcgcacaa caggtgtcca gtctttgaat gccttttcaa gaaaattgaa
     4861 gtcctgaccg aaatctgcca tgaaaatata tttaaagccc atgcgccagg tgctgatgtc
     4921 cgtgttgtgc cgtccgatct tctcggtata ctgcgcccag tagtccgtac catgccagta
     4981 gatcagtgat acgccgaaaa gagtggcggt aatcacggcg aagctcagga aaaaataaaa
     5041 ataacgccta tcgatttctt tcagccagag ccctcgcagg gtcgtcaccg cgcctgccag
     5101 agcaagagcg acccaggaaa taaaggtgaa cagctgaagg aaagcggaat gaccgccggc
     5161 tcctgaaacc atctggttga aaaagccgct gatgctgcgg tccggtgatc cgatccaggg
     5221 cagagcaaca gcggacacaa aagcgtagct gccccagaga aggaggaaaa taaaaagaaa
     5281 aattgttgct tttacaacaa gccgctcgcc cagactgcgt ttctgcagcc cgagagaaag
     5341 gtattttctc cgcagcagca tcaggcagta atccagaatc agccagaaaa aacgcgcgcc
     5401 gatgccgaag agcagtacag ccggaaaaat gcgggataat gtcgcatagc cggtcagcgc
     5461 acctgccagc gcaaaatgct ttttccgaat gagacacacg ctcatcacca ggcacatgac
     5521 ccagtccgtg cgcagatatg cacccttcag gtgccagtat ttcatcatat agcaggtgcc
     5581 caacagcgta atcatcagaa aggcggtgcg cggtccgaaa gcccagagta ccatcagaaa
     5641 agtgatgagg atgagcagcg gatccagcac ggcaagtatc atcatgttgc ggtcgctgtc
     5701 cgtgctgacc cggttgctca gcagaccgcc cacaagcatt gtccagacgg gtgtaccgtt
     5761 atatcccttg tcccgcagga caccgctcca ccgggactga atcatacggt ctttaaacca
     5821 tacaatgtct tttttaaact cctcccaacg ctcaggggtg aagcgggatt tatacttatc
     5881 cgcctgggca agcaccgttt tagcactcac tttttttccc gtattcaagt cagtaatggt
     5941 gcccgagctg tgcttccatt tcattcccgt ctcggaatcc gccaccagtg atgccatgta
     6001 catgtcaccg tagcccacct cccgggcata tttggtgcct atataatagt gatagaactc
     6061 ataggcattc agatagctgt caaagcggta ataatcggtg tagcggtaat tttccacggc
     6121 aagcaccgcc acggcaaaaa gaggtgccag cagaaaaccg cgcaggaaaa ggagtcgttt
     6181 ctccccaagc caggctttga cgctgttccg gttcaggagg taatagcatg ccatgataat
     6241 catcgccgcc accggcagga aaaaatgaag cgggtagttg gcggagatct gattcagaaa
     6301 agcaagaaaa gtctgtacca tgaattgtcc cagtgctttt tgcagttggt gcggcggcac
     6361 ggatgccgac tgcctgattt gccggatgca gcgttatagc ctgcacccat ttttaccagc
     6421 ccaagtataa cataaggctt cctgcctgat ttctgtgccg attccgaggc tcagccttcc
     6481 ggcgcccaac cgggttcgta atcacgctgc cagagtgcgg cggccgccgg gttatccaga
     6541 atatggccct gtccatcaca atgcacggca ctgtctgtcc ggtaggcaat gttgcccaga
     6601 tggctgagca gcgtgctcct gaaagcggtc tcgatttccg cgtgaagcgt gtccggtgta
     6661 ccgtcctgaa tcgctgcaag aaaattatcc cgatgctcct catcgcccag cgggccggat
     6721 tgctgatcca gcaccttgcc gtccggatca cgaagcgtat aatcctgact gcgcagtgtg
     6781 agactgcctt tttcgccgaa aaagcacaca cccacgtctg tgtcaatccc cggacggttg
     6841 cagctgagcc cttcccacaa aaccattttt tctccgggaa attcatagga gaccgtgagc
     6901 gtatcgggcg tttcctgatc gtcatcgaag cggaagcgcc cgccccgggc ggtgacccgg
     6961 ctcggaaaat ccacctgaag gccccagcgg gcgaggtcga gcatatgtgt gccgttgttg
     7021 cccaactcgc cggtgcccca atgccagaac cagtgccagt tgtaggggat cacgttgtct
     7081 ttatagggtt ttcgaggcgc aggaccctgc cagaggtcgt agttcagact tgcaggcacc
     7141 gctgcttcaa cacctttacc gatggttccc cgaacggagg cataccaact gcgcacatag
     7201 gaaacagcgc ctatttcgcc ggcgtgaagc cgtgcaatgc cttcccggat gacgggcgaa
     7261 gagcgacgct gcgtacccat ctgcacgacc cgtccgtatt tgcgtgccgc tgccacgagc
     7321 agctcgcctt ctctcgggtt gtggcagcat ggtttttcca cgtagacatg cttgcccgcc
     7381 tgacaggcga gaatcgacag cggggcgtgc cagtgtaccg gcagcgccat gacaacggcg
     7441 tccacttcag gatcatccag aatgtgcctg aaatcacccg tcctttccgg aaggtagcca
     7501 aaagcctttt caaagcccgc tgctgccgct ccgagccgct ccgtgtccac atcacagaga
     7561 tagcggatcc ggcattgatc agaggcaccg aaagtgtggg caagcgcctt gccccggctc
     7621 atgccgatga ctccgatttg tatcttgctg ccgggaagga ctgttcgggc acccgctgag
     7681 gaggcaacgg caagggcggc gcttccggct gctgcctgag ccatgaaaga acgtcggctg
     7741 atggatgtac tcatgaaaag actcctggtt aatggacagt cattcaagag tagtcatttc
     7801 cggcaggaaa tttcaaggaa cacccgcacg tttcagtgcg ccggcttccg tgcggagacc
     7861 gaaaggctca cgatgtaatc gctcagtgac tcaccttcgg gcatgtggtc acggatttca
     7921 ccgtagagcg acgccttcaa gcctgtcatt gtctcaatat aatcgctttt taccgtcagt
     7981 tcaatgtcgg taaatcccgc ttcttttgcc agcgcctctg tatcctccac aaaaacagcg
     8041 cctgccatac agccgatcag ggcggtagac tgttctttta catccggagg cagggatttc
     8101 aaaagcgcca tatctgaaat ggccgcccga ccgcccggct tcaacacgcg gaaaatctct
     8161 tgccagacct gctttttgtc cggggaaagg ttgatgacac agtttgaaat caccacatcg
     8221 gcggcgttgt cggcaacggg gaggtgctcg atttcaccca ggcggaattc aacattgtct
     8281 tttccggtgc gccgccggta ggtttcgatg ttgcggcggg ccaggctgat catttccggg
     8341 gtcatgtcca cgccgatcac ccggccgtca tcgcctacct tttctccggc gataaaaaca
     8401 tcgaagccgc cgccgctgcc cagatccacc acccactcgc ccggtttaag tgcggcgatt
     8461 gccgtgggat tgccgcagga gagacccatg tttgcccctt ccggaagaag ggcaagctcc
     8521 gcatcgctgt aacccactgt ttttgccagc gcatccgcat tatccccgca gcagcaggtg
     8581 cgtttcggag cgcagcagct tccttcttcc tgtgcaattc tgccataccc ttcgcgtacg
     8641 tgggcgcgct gtgattcttt gtcctgatcc atacgaattg ttcctttcat tttaacagca
     8701 gtaccggtcg cacaattctt ttttatcctg cttcaatacc cggacgagct gttttttgtc
     8761 tttttttaca atatcggcac tcttcagact gcggctcacc cagtgcagca actcagcagc
     8821 aagatcgtcc ggtcgtttat caggcaggcg gtaaaacttc cagcgccctt ccttccgcga
     8881 aagaacgaga tccgcctgta cgagaatgga catatgccgt gatactgtcg acggcgccag
     8941 atccagcagt gcaatcagct gacagagaca cagctcctct tccgccagca gcagcagaat
     9001 ccgcgccctg gtttcgtcgg caattgcctt tgcctgcatc ataaaaacct tcattgttac
     9061 ttccttaatt cgttaagtga cgaagtgtag tgtgccataa aagcagcttc atttaaaaaa
     9121 atataagcaa gcacatgcgt ccggtgcctg gagtcatccg gtcccggttt cttgattcac
     9181 gctccttctg ttcgataagt tatactatgg tgtgtgctgt ttttcagcag ctataccaaa
     9241 agaattggcg gagcggcgtt gtaacccgtc tcctgaaaag cggtttttcc ccggtcggct
     9301 gtgtcgaaag gaatagttta tgttcagaaa aaaaatgatg ctctggattc cagtcgcagt
     9361 gcttcttctg cttaccggat gcaataccct ttcttctgca cctcagatta cgaaagcggc
     9421 gattacaccg gaggttcttc gtcccggcga tacggccgtg ataaccatgc agattcatga
     9481 ccgttcccga gtcgtgaagc gtgtggaagg gattgtgctg gaagatgccc gtatcacctt
     9541 caaactgcgt gacgacgggg cagaacctga tgaaaaggcg aatgacggga tctggtccat
     9601 gcaggtggat gtaccttttc aggcgcctcc cggcgaattc cgcctggaat tcacagccta
     9661 cggacaggac ggaacgccta ttcctgtgcg ggatcgggac gggcatattt ctccgctgca
     9721 gcaggtgatc ccgctcagga ttgaatacca gcaataaaca atggctgctt ttctgttatg
     9781 gatcagctgt ctgacagcgg ctgtcgctga tccgcttccc gatgtcgttc tgctttccgt
     9841 ggacaccctc cgtgcggatg tgctcggctg ctacggctct ccgcatcctg ttacaccgga
     9901 actggatcgt ttttccgaaa cagccctgat tttcgatgat gcgcagtgtg aaattccgct
     9961 tacaggtcct tctttttcag cgatgtttac aggtcgcttt ccccgcgagc tgggcgtcac
    10021 tcggaacggg ctgaaacttc cggaagacat gcgcaccctg acccgggttt ttcgggaaca
    10081 tgggtatcac tgcctcgccg ttcagagcaa ctggacatta cgaggcaaac tgtgcggatt
    10141 gaatcgggat tttgatgtgt atgatgacga actgccgcaa aaacgatggg gagtgtaccg
    10201 gggtgaacga cgcgcggctg acctcttgaa agcggcagaa aaacatctga aagaacgccc
    10261 ggaaggaaaa ccgcttttcg cctggatcca tttcagcgac cctcatgctc cttatcgttt
    10321 ccgaagagat tttgatcccg cgggcaccga tttcagaaag ggcgataaaa aagaattaac
    10381 ccgcgcccga tacatgtcgg aggtgaatta ctgtgacagc catattggcg cgcttttaag
    10441 agcccttccc gaaaaagccc gcattctttt tgttgccgac cacggcgaaa gcctccatga
    10501 ccacggttat ctggggcacg gccgtcatgt atacagcgaa tgcacccgca tcccgcttat
    10561 gattcgctcg ccagaactga agcccggccg cagtgcttac cccgcctctt cgctggatgt
    10621 gggtgtcacg ctgctggctt tggcagggct tccgccgctg ccgggtatgc ggggcgtgaa
    10681 cctgtgtcgg gcggaagctc tgccgcggcg tccccgcttt acggaaacct atggcggact
    10741 ggcaccgggc atcccggggg tgcgccaaat gctgcaggac acccagccgt tacgctgtgc
    10801 agtttccttt gaaggctgga aactgcacgt tttgtcttcc cagcagatgg aactgtacca
    10861 cctgaccgag gatcctggcg aagaaaaaga cctgcgttat cgctatccgg agcgcatcaa
    10921 agttctgaag gcgctgctgg atgactggga ggcgacctgt ccggcgggac gtgcagaaga
    10981 tgcatcgctg aaccgggacg acctggaggc gctgcgcagc ctgggctata ttgaataggc
    11041 acgcctgcgc gaccgggtca ttcctttgtc ggcagaggca ccggaaccgc ccagacttcg
    11101 cggcggcggg gaccgtcaaa ctcgcagaaa aagacggact gccatacgcc gaggcagggc
    11161 ctgccgcctt cgatgataag tgtgcagccg gcgcctgtca tgcttgcttt tacatgggct
    11221 gccgtattgc cttcagcgtg ccggtaggcg gggtcatgcc agggcaccat acgatccagc
    11281 accagttcca tatcccgcgg aacatccgga tcggcatttt cgttgatggt aatcccggcg
    11341 gtggtatgag ggacatggag ctgtagtatg cccgcctcaa gtccgcattc cgtcagcagt
    11401 tttctgatct gactgtcaat attgatgaac tgggtccgtg cacgtgtttc cacagaaaaa
    11461 cggtacatag tcagtactcc ctgtcgcgca gattgcgcag gccgaaatac atgggcagtc
    11521 ccacagcagc cagcgtggtg aggagaccga cgctgatcgc tgccgttaaa gcataatgaa
    11581 agagagcggg tgaggcaaag ccgcccagtg cgttccattg gagcacgagt gtctgagcgg
    11641 cgatgatcac agtgatatag gcgatgctga gcaacagatt cagggttccg ccgagcccgc
    11701 ttacaatacg ggcggggttg tcttcatgaa aactgggata gagagcgcca agccccacgg
    11761 caagccctgc cagtccgaaa ttagcaacaa caacactgta gacagtcatc cagaaaaaaa
    11821 caggctccag tttcagcatg gttgcagaca gaaatgaaag tccgaccgta aaaagggaag
    11881 tgacacagac actgagccag aatttctgcc agacgagtcc ggtaaagcgg aagggtgcaa
    11941 gtccgataat ccagaatcgg cggccctcca ggctgacgag gggaaaaacg aagcggctgg
    12001 tcagcgtgga cagaatcagt gtgaccgatc cgacgttcag gcaggcaatc cagctccgcc
    12061 acatggcatc ctcaaaataa cgggaactgc tgcgcagatt agcgatgtaa atcgccatga
    12121 taccgaagaa aatgacaaac tgcgaccatt gggtaggatc ccgccagaag agctttatgt
    12181 cttttacagt aagagaacgg tagggctccg gtatgaagga aagaaagcgc tccagccggt
    12241 tcagaatt
//
LOCUS       DAOPWB010000094        12196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5315_length_12196_cov_5.543942, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000094 DAOPWB010000000
VERSION     DAOPWB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5315_length_12196_cov_5.543942"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKN92_09140"
     CDS             <1..473
                     /locus_tag="PKN92_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ48997.1"
                     /translation="QVQTLLGKGETFVEDTTTLVKESTETVQDIRSHASSLVSSLETR
                     STEVASLIKKLEQLVEVSTVRAEQLDVQKLQEQFSELLAQVTNAATQLDTTVANMDLI
                     AQDIVHQAGNVEYSLRGTLAEFRDSLESVRIMINQIKDDPSSLIRGRGRIKEQD"
     gene            489..1133
                     /locus_tag="PKN92_09145"
     CDS             489..1133
                     /locus_tag="PKN92_09145"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNZ48998.1"
                     /translation="MSAKSLIRTGTNIAAGMLLLLILQGCLSTPVVQKTLYYSLTPHL
                     EMEKKPPLEYTLGIRPLPNAKSYGLPIAYLDNSFQIGYRSREEWVEQPALVVTRAIQD
                     AIASTGRFADSGNAADMVRPDLIMTGELRAFHENRTEEKPKVEITVHFQVRPSTVKGL
                     LWSGTLTESEYLESGSALDVARAMNRVLERLARNVADAVCSIELPPREEFFKNP"
     gene            complement(1143..2822)
                     /gene="ettA"
                     /locus_tag="PKN92_09150"
     CDS             complement(1143..2822)
                     /gene="ettA"
                     /locus_tag="PKN92_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNZ48999.1"
                     /translation="MAEQYIFTMLGLNKFYGQKQVLKDINLCFFPGAKIGIVGENGSG
                     KSTILRIMAGLDDDFRGRAELSRGYTARMVAQEPALDPDKTVRATLEEAFGEIQALLA
                     EFDALSMSMAEITEDDEMQKALDRMGVLQDKLDAADAWNLDQRLNQASEALCLPEDDR
                     LVGTLSGGEKRRVALCRALLEKPDLLLLDEPTNHLDAETVDWLETQLKEYPGTVLIVT
                     HDRYFLDNVTRWILELEGGRGIPWEGNYSSWLEQKLSLLAENEKASTPRGRALQRELD
                     WIRMSNKARKDLSQARLGAYEQLIARESAEARGDSAVIHIAPGPPLGDQVIEVKGVSK
                     SFGGQMLFKDLDFIVPRAALVGIIGPNGTGKTTLLRMLLDHEKPDSGSIKAGSSVRFS
                     YVDQERSSLKDDVSLLDEVGGGLDEIELGKVRIPLRKYLAQFGFRGADQQKMAGQLSG
                     GERNRCNLAKLLKEGGNVLLLDEPTNDLDVNTLRLLEEALLNFSGCVLVISHDRFFLD
                     RICTHLLVFEGEGQVRWFQGNYQDYEAWRLREFGTRLFENRRARYRKVIKG"
     gene            3076..4881
                     /gene="lepA"
                     /locus_tag="PKN92_09155"
     CDS             3076..4881
                     /gene="lepA"
                     /locus_tag="PKN92_09155"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNZ49000.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLIEFTKTVDERKMTEQTLDNM
                     DIERERGITIKSVAVRMEYLADNGENYILNLIDTPGHVDFAYEVSRSMAACEGALLVV
                     DAAQGVEAQTLANAYKAIEQDLEIIPVINKVDLPAADVEGVRRQIEEIIGLDAQNAVL
                     VSAKTGLGTKDTLESIIERIPPPEGQREGPLRCLIFDAVYNTYRGVIVYLRVVDGSIA
                     PGDKIVFMATGIRHEVSEVGALQPHLVPRDALHAGEAGYLICNIKTLSDTKIGDTVTH
                     ADRPAKTPLPGYEEAKPVVFSGLYPAVSTDYEELREALEKLQLNDASFQFHADSSDAL
                     GLGFRLGFLGLLHMEIVQERLEREFGLTLVTTMPNVAYDITLTKGEKITIEKASQMPP
                     PGEIAVIEEPYIEADILCPTQYLSAIIELCKRKRGVHVRVDYIDGNRCMAIYRMPLAE
                     IVLDFYDKLKSITRGYGSLDYKLIGNQPGDLVKLDILLNGEHVDALSTIVHREHAVRL
                     GRALAQKLRKLIPRQQYEVAIQAAIHNKIIVRESISALRKNVTAKCYGGDISRKRKLL
                     EKQREGKKRMKQIGTVEVPQEAFMALLRVNEDDNT"
     gene            4868..5893
                     /gene="lepB"
                     /locus_tag="PKN92_09160"
     CDS             4868..5893
                     /gene="lepB"
                     /locus_tag="PKN92_09160"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNZ49001.1"
                     /translation="MTTPEALPGKTLRFFRRLLEQITGPWTWKNLGGWIIIILLLLFV
                     KGCIIDQYTIPTGSMDPTLHGDPRLFHGDRVLVNKWRFGPRIPFTTHRLARWDTPDRW
                     DIVVFRSLEKETQQPVLIKRVIGLPGERVRIRDGSIFINGEKCPPPEQLKDTLHYYTE
                     LKFTDVEKKRQFLKLAQKNQPLAILNPEEPPVKHFYQEMKRFHEKAEKLDVDALSDEK
                     VLDFCRELDPRTLQLIDQVYVFYQPPMPYGCRDEDEYTLVPEGHYFLLGDNSAASLDG
                     RMYGWVPHNHLYGSAFAVWWPWARRQDFTGFSHTWWGMALLYGIPALLLLWELSGYVR
                     KRKKENK"
     gene            5911..7182
                     /gene="tyrS"
                     /locus_tag="PKN92_09165"
     CDS             5911..7182
                     /gene="tyrS"
                     /locus_tag="PKN92_09165"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNZ49002.1"
                     /translation="MSFFNELHWRGFVNQMTHPELPDILDKESVTLYCGFDPTAYSLH
                     IGSLLPIMGLAHFQRAGHTPIALVGGGTGLIGDPSGKAQERTMLSREEVEHNCEGIRA
                     QLERFLDFKGSHAAIMVNNADWLCEVKLLDFMRDIGKFFSVNALLNRDSVRARLEDRE
                     SGISFTEFSYILLQAYDYLYLNKKYNCRLQIGGADQWGNIVSGMDITRRLNNASTFGL
                     TFPLVTKSDGAKFGKSEKGNIWLDSTRTSPYQFYQFWINQADADVSRFLRYFTFLPET
                     EILALDEQIREAPQERAAQKRLAEEVTRLVHGETALANAVTASNAMFGGDWTALDAQT
                     LLDVFSEVPHTEMPLETLQRTELLNDLLVHAEVFASKGEARRLIRNGGLYVNNMRIEK
                     EDVTCNALPLLAGNLMVARIGKKSYHLIRFV"
     gene            complement(7256..7342)
                     /locus_tag="PKN92_09170"
     tRNA            complement(7256..7342)
                     /locus_tag="PKN92_09170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7306..7308),aa:Leu,seq:aag)
     gene            7498..8163
                     /locus_tag="PKN92_09175"
     CDS             7498..8163
                     /locus_tag="PKN92_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ49003.1"
                     /translation="MDSSPILEIRDLDGMAGNICLFRKLHLTLDPGEKCTLSGASGSG
                     KSTLLKMIMGFLPVPADTIFITGEELTAKSCWRLRKCVACVSQEPEIGKGKVCDILAA
                     PFQYRSNRSLSFELKEAQQLFECFLLPADTLQKDMEVLSGGEKQRVALTAALLLKRKL
                     LLLDEVAAALDARSKEAVRAYLASLKNTAILSVSHDVGDFLLSSRLEWMEDLQKGGGN
                     ARD"
     gene            8150..8947
                     /locus_tag="PKN92_09180"
     CDS             8150..8947
                     /locus_tag="PKN92_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ49004.1"
                     /translation="MPVTDIDVVRLAAGYGLLLIPLGIFLWHRVPLIGTTLVSIVRMT
                     VQLLFIGFYLQILFDLNNIWLNIMWLAVMIAVADASILRGCDLNFRIFAIPVFVALLL
                     GILLPVFIFLTVILYPAPALDARFLVPIFGMVLGNSLRAVIVGLQHFYDALRRREKEY
                     FFFLSAGAGRQEALMPFLKETLQVSMAPTLANMATMGLVALPGMMTGVMIGGISPAAA
                     IKYQIAIMIAILSGTSLAATLAVYFSLRKSLTPWGSLNRSVFTRLNQ"
     gene            complement(8941..11610)
                     /locus_tag="PKN92_09185"
     CDS             complement(8941..11610)
                     /locus_tag="PKN92_09185"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49005.1"
                     /translation="MQDKAANNELIAFLDSVKEGAGRCNESHQALVSQLTQLKTTFQA
                     LEAQLESAYEQQASVFAKHASAYEMLASQVQGLPEGGDGLHKRILDLEEEVHHKEKQL
                     AEQHRKIAALEAQVSAGGDEKVQHYIKGLEEHLQKRNETIGVARRRILRLQEKIEGMA
                     AERTSAVQRAEDLELQLERKNKALQSSEDRFNELRKQLEENSGSDQENQAQIQALERL
                     LADKEEALSGAEERNRQLQDDVSSRSRDLASVQERIHALEQADLELRQEMEEKQAEMS
                     ALQSHYDALKQQAAAFEESKQDVAHQLQEQAAAAGRFAEEKAEMEEQLVAAAREKAAL
                     LAEMDVLRDAAARMDSDTAAGENRQQELEAECNRLNSELNAAREALDGFQQRLSEAAK
                     THAEQEGIVVGLQQEVEREKAATAAAREYLAQVQESAEKVSAQLSEQQSQISFLQSEL
                     GDRETALDTAERRAELAEDKLEKLLSESGDAEARVATLVGELNAHKERTETLESALAA
                     AQDKIADLTEEKEEAARNAADARQALQTIAEDTETAERGAAEWKTKFTDLERQQQQLK
                     KELDEKSEALSRTQAQLLSSEEMRQTLEASAKKSLDAQNSFESEKEALQKINEQLKQE
                     VQDLNNKVKDLHVELEQGASPEEIENMRSALEEEKRKTAQLQERLDEELSKGTKAGLA
                     RQLADALRDAEDARAELRAMKRLPADVTGEVETVAPSADSARGALPVREVINSLPRQK
                     QRNLGEVLVALKCITTDQLSDAISEQKLHPESDFVAVLVKMGFMTPASLAEIISVVHQ
                     CPLVSISAAGLDPKAMRLVPERLAKTRCCIPRQVLGKELVLVMENPGNLLVIEDIERL
                     TGLRVTPNVAPRDQILKAIDAFYTAEPESGSPY"
     gene            11996..12175
                     /locus_tag="PKN92_09190"
     CDS             11996..12175
                     /locus_tag="PKN92_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49006.1"
                     /translation="MRSTFLFCLSLAMGAAAWGDAPSFMMENDRIKIVLDAATGGFAS
                     IYDKQKADSRLKCNY"
BASE COUNT         2790 a         3382 c         3109 g         2915 t
ORIGIN      
        1 atcaggtaca gacgcttctg ggcaaagggg aaacttttgt tgaagacacc accacgctgg
       61 tgaaggaaag cacagaaacg gtgcaggaca tccgcagcca tgcaagcagt ctcgtttcca
      121 gtctggagac cagaagcacc gaagtcgcca gcctaatcaa aaagctggag cagctggtgg
      181 aagtgtccac tgtccgggca gaacagctgg atgtgcagaa gctgcaggag cagttcagtg
      241 aactgctggc gcaggtcacc aatgcagcca cacagttgga tacaactgtg gcaaatatgg
      301 acttgatcgc tcaggatatt gtccatcagg caggcaatgt tgagtattca ctgcggggca
      361 cgctggctga attcagagat tccctggaaa gcgttcgcat catgatcaat caaattaagg
      421 acgacccttc ctccctgatc cggggacggg gcagaattaa agagcaggat taattcggga
      481 gacacaccat gtcagccaaa tcgcttattc gtacaggcac gaacatcgcc gccggaatgc
      541 ttctgctgct cattttgcag ggatgtcttt ccaccccggt tgtgcagaag accctctatt
      601 attcgctgac gcctcatctg gaaatggaaa aaaaaccgcc gctggagtac acgctgggga
      661 tccggccgct gcccaatgca aagtcttacg gactgcccat tgcctatctg gacaacagct
      721 tccagatcgg ataccggtcg cgggaagagt gggtggagca gccggcgctg gtggtcaccc
      781 gggctattca ggatgccatc gcctctacag ggcgttttgc tgattcgggc aatgccgccg
      841 atatggtacg gcccgacctg attatgaccg gtgagctgcg cgctttccat gaaaaccgga
      901 cggaagaaaa gccgaaagtc gaaatcaccg tacattttca ggtgcggccc tccaccgtga
      961 aagggctgct gtggtccggc accctgacag aatcggaata ccttgaatcg ggcagtgccc
     1021 tcgatgtcgc ccgcgccatg aacagggtac tggaacgcct tgcgagaaac gttgccgatg
     1081 ccgtatgctc cattgagctg ccgccccggg aagaattttt caaaaacccg tgagtaaagc
     1141 catcagcctt tgattacttt gcgatagcgt gcccgcctgt tttcgaaaag gcgggtgccg
     1201 aattcgcgga ggcgccacgc ttcatagtcc tgataattgc cctggaacca ccgcacctgc
     1261 ccttcgcctt cgaacacaag caggtgggtg cagatccgat ccaggaagaa acggtcgtgg
     1321 ctgatcacca gcacacagcc gctgaaatta agcaatgcct cttccagaag ccgcagcgtg
     1381 ttcacgtcca gatcattggt cggttcgtcc agcagcagca cattgccgcc ttctttcagc
     1441 agtttggcga gattgcaccg gttccgttct ccgccggaaa gctgccccgc cattttctgc
     1501 tgatcggcac cccggaaccc gaactgtgcc agatattttc gcagaggaat gcgcactttc
     1561 cccaattcta tttcgtccag ccccccgccc acttcatcca gaaggcttac atcatctttc
     1621 agggaagagc gttcctgatc cacataggag aagcgcacgg aactgcctgc tttaatgctg
     1681 cctgagtccg gtttttcatg atccagcagc atgcggagga gcgtggtttt tcctgtgccg
     1741 ttaggcccga tgatgccgac cagtgccgcc ctcggcacaa taaagtcaag atctttaaaa
     1801 agcatctggc cgccgaagct cttgcttacg cccttcactt ctatcacctg atcgcccagc
     1861 ggcggcccgg gcgctatatg gatcaccgcg ctgtcccctc ttgcctccgc gctttcccgg
     1921 gcaatcagct gctcatatgc gcccagtcgt gcctggctca ggtctttgcg cgctttattg
     1981 ctcatccgta tccagtcaag ttcacgctgc agtgcacgcc cccggggcgt ggaagccttt
     2041 tcgttttccg ccagcagact cagtttctgc tcgagccagg acgaataatt tccttcccag
     2101 ggaattcctc tgccgccttc cagttcaaga atccagcggg tcacgttgtc cagaaaatat
     2161 ctgtcatggg tcacaatcag cacagtaccc ggatactctt tcaactgggt ttccagccag
     2221 tccaccgttt ccgcatcaag atggttggtg ggttcatcca gaagcagcag atccggtttt
     2281 tcgagcagcg ctctgcacaa ggcaacccgg cgcttttcac cgccgctcag tgtgcccact
     2341 agccggtcgt cttcaggcag gcacaatgcc tcgctcgcct gattcaaccg ctgatcaaga
     2401 ttccaggcgt cagcggcatc caatttatcc tgcaggacgc ccatacgatc gagtgccttc
     2461 tgcatctcgt catcctctgt gatttccgcc atactcatgg acaaagcgtc aaactcggcg
     2521 aggagcgcct gtatctctcc gaaggcttcc tccagggtcg cccgcaccgt tttgtccgga
     2581 tccaaggcag gttcctgggc aaccatccgc gctgtatacc ctctggaaag ttccgccctg
     2641 cccctgaaat cgtcgtcaag tcctgccata attctaagga tggtggattt acccgagccg
     2701 ttctccccca caatccctat ttttgcacca gggaaaaaac acagattgat atccttcagc
     2761 acctgtttct gaccataaaa tttattgagc ccgagcatgg tgaaaatata ttgttccgcc
     2821 atcgacagat cctccgaaaa aaaactgatg taaaggagac gccccttatt ataccgaatc
     2881 tgtgccgtgc cgcaagggca gcccttccgg agggcatctt tccccctgcg ctgcaggagc
     2941 aaaaaagggt ttttgcgtgt atccgcttca gatatgctat tatatgtagc gatgattggg
     3001 gcaggggaag gcttattaac acaacttttt gcctgaaaat caggcggacc cggcttatta
     3061 aatatgacag caccgatgaa aaatatcaga aatttctgta ttatcgctca cattgatcat
     3121 gggaagtcca cacttgccga ccgcttgatc gagttcacca aaacggtgga cgaacgcaaa
     3181 atgaccgagc agacccttga taatatggat attgaacggg aacggggcat taccattaaa
     3241 tcggtagcgg tccgtatgga ataccttgcg gacaacggtg aaaattacat tctgaacctc
     3301 attgacacgc ccggtcatgt ggattttgcc tacgaagtat cccgctccat ggcagcctgt
     3361 gaaggcgcac ttctggtagt ggatgccgca cagggcgtgg aagcgcagac actcgccaac
     3421 gcttacaaag ccattgaaca ggatcttgaa attattcctg ttatcaataa ggtggatctc
     3481 ccggccgccg atgtggaagg cgtccggcgt cagattgaag aaatcatcgg gctggatgca
     3541 cagaatgccg ttctggtcag tgcgaaaacc gggctgggca ctaaagatac tctggaatcc
     3601 atcatagagc gtattccgcc gcccgaagga cagcgcgaag ggcctttgcg ctgtctgatt
     3661 ttcgatgccg tatacaacac ctacagaggc gtgatcgtct atctgcgtgt ggtggacgga
     3721 tccatcgcac cgggcgataa aatcgttttc atggcaacag gcatcaggca cgaagtgtcg
     3781 gaagtcggcg cgctccagcc tcatcttgtg ccccgggacg cactccatgc cggagaggca
     3841 ggctatctca tctgcaatat caaaacgctg agcgacacca aaatcggcga cacagtcacc
     3901 catgccgacc ggcccgcaaa aacaccgctc ccgggatatg aagaagctaa gccggttgtt
     3961 ttttcagggc tctatccggc tgtttccacc gactatgaag agctgcggga agcgctggaa
     4021 aagctgcagc tgaacgacgc ctcctttcag tttcatgccg acagttccga tgcattggga
     4081 ctggggttcc ggctgggctt tctcggactg ctccacatgg aaattgtgca ggaacggctg
     4141 gagcgggaat tcgggctgac cctggtcacc accatgccca acgtagccta cgacatcacc
     4201 ctgacgaagg gcgagaaaat tactattgaa aaagcgtcgc agatgccgcc gcccggtgaa
     4261 attgccgtta tagaagagcc ttatatcgag gcggatatcc tctgccccac ccagtatctc
     4321 agtgccatca ttgagctctg caagcgaaag agaggcgtgc acgtgcgggt ggactacatc
     4381 gacggtaacc ggtgcatggc catataccgc atgcctctgg cggaaatcgt gctcgacttc
     4441 tacgacaaac ttaaaagcat tacccgcggc tacggctctc tggattacaa actcatcggc
     4501 aaccagcccg gcgatctcgt caaactggat attctcctga atggtgaaca cgtggatgct
     4561 ctgtccacca tcgttcaccg ggagcatgct gtcaggctgg gcagagcatt ggcacagaaa
     4621 ctgcgcaaac ttattccgcg ccagcagtat gaagtcgcta ttcaggccgc catccataat
     4681 aaaattattg tcagggaaag tatttcggcg ttacgtaaaa acgtgaccgc caaatgctac
     4741 ggtggcgata tttcacgcaa aagaaagctg ctggaaaagc agcgggaagg caaaaagcgc
     4801 atgaagcaga tcggcacggt tgaggtgccg caggaagcgt tcatggcgct gctccgggta
     4861 aatgaagatg acaacacctg aggcgctccc cggaaaaacg ctgcggttct ttcgccggct
     4921 gctggagcag atcacaggtc cctggacctg gaaaaatctg ggcggatgga taatcattat
     4981 tctgctgctg ctctttgtaa aaggctgcat catcgaccag tacaccattc cgaccgggtc
     5041 catggatccc acattgcacg gcgatccccg gctttttcac ggcgaccggg tgctggtcaa
     5101 taaatggcgt ttcggcccgc gtattccttt taccacgcac cgccttgccc gatgggatac
     5161 acccgaccgc tgggacatcg tcgttttccg atccctggaa aaagaaacac agcagcctgt
     5221 cctgatcaag cgggttatcg ggctgcccgg cgaaagagtc cgtatcagag acggcagtat
     5281 ttttattaat ggagaaaaat gccctccccc cgaacaactc aaagatacac tgcattacta
     5341 cacggagctg aagttcacgg atgtggagaa aaaacggcag ttcctgaaac tggcgcagaa
     5401 aaaccagcct ctggcaattc tgaaccccga agagccgccg gtgaagcact tctaccagga
     5461 aatgaagagg tttcatgaga aggcggaaaa actggacgtt gatgcactca gcgatgaaaa
     5521 ggtgcttgat ttctgcagag agctagaccc caggacactg cagctcattg atcaggtcta
     5581 tgttttctat cagccgccca tgccctacgg gtgcagagat gaagacgaat atacgctggt
     5641 gccggaaggg cactatttcc tgctcggcga caacagcgct gccagtctgg acggacgcat
     5701 gtacggatgg gtgccccata accaccttta tggaagcgcc tttgccgtgt ggtggccctg
     5761 ggcgcgccgt caggatttca ctggcttttc acatacctgg tggggcatgg cactgctcta
     5821 cggcataccg gcgctgctgc tcctttggga attgtccggt tatgtccgca aaaggaaaaa
     5881 agaaaacaaa taaagaaagg aaaaaagaaa atgtcctttt ttaatgaatt gcactggcgc
     5941 ggttttgtca atcagatgac ccatccggaa ctgccggata ttctggataa agaatccgtc
     6001 accctctatt gcggctttga tcccaccgca tacagcctcc acatcggaag cctgctgccc
     6061 attatgggac tggcgcattt tcagcgtgcc ggacacacac ccatcgcgct ggttggcggc
     6121 ggcacaggcc ttatcggcga tcccagcgga aaagcgcagg aacgcaccat gctcagccgt
     6181 gaagaagtgg agcataattg cgaaggcatc cgtgcgcagc tggaacgttt tctggatttt
     6241 aaaggctccc acgcggcaat catggtcaat aatgcggact ggctgtgcga agtgaaactg
     6301 cttgacttca tgcgggatat cggaaagttt ttctctgtca atgccctgct caaccgcgat
     6361 agtgtccgtg cccggctgga agatcgggag tcaggcattt ccttcaccga gttctcctac
     6421 atcctgctgc aggcctatga ttacctctac ctcaataaaa aatacaactg ccggctgcag
     6481 atcggcggtg ccgatcagtg gggcaatatc gtatcgggca tggatatcac acgccgcctg
     6541 aacaacgcct ccactttcgg actgaccttt cctctcgtaa ccaaatcaga cggcgccaaa
     6601 ttcggcaagt cggaaaaagg aaatatctgg ctcgacagca cccgcacctc gccctaccag
     6661 ttctaccagt tctggatcaa tcaggcggat gccgatgtat cccgcttcct ccgctacttc
     6721 actttcctcc ccgaaacgga gattctcgcg ctggatgaac agatcagaga agcacctcag
     6781 gaaagagcgg cgcagaaacg gcttgccgaa gaagtgaccc gcctcgttca tggggaaacc
     6841 gcactcgcca acgccgtaac cgccagcaat gccatgtttg gaggcgactg gactgcactg
     6901 gacgcacaga cgctccttga cgtcttttcc gaagtgcctc atacagaaat gccgctggaa
     6961 acattgcagc ggacagaact gctgaacgac ctgctggtgc acgccgaagt gttcgccagt
     7021 aaaggcgagg cacggcgcct cattcgcaac ggcggtctct atgtgaacaa catgcgcatc
     7081 gaaaaagaag acgtcacctg caatgcactt cccctgcttg ccggcaatct tatggttgcc
     7141 cgaatcggaa agaaatcata ccatctgatc aggtttgtgt gatcggatcg gcaggctgca
     7201 taaaaaaagc gccggactgc atgggctgtc cggcacctga atggagaaaa aaagatggtg
     7261 cccgaggcga gaatcgaact cgcaccccat cgctgggact agatccttag tctagcgtgt
     7321 ctgccatttc caccacccgg gcgtgagagg aacggtgaaa ctttaccaaa aaagtgcagg
     7381 aaaaatcaaa ccgattctct tcgcggttcc tgaaaaaaat ggtatgattg aaaagaaacc
     7441 ctgttcttcc ttcgtgactg atttatcccg gcatctgaaa acccatgcag gccttcaatg
     7501 gacagttcgc ccatccttga aatccgcgat cttgacggca tggcaggaaa catctgcctt
     7561 ttccgtaaac tccatttaac attggatccc ggcgaaaaat gcacattgag cggagcatcg
     7621 ggctccggga aaagcacgct cctgaaaatg attatgggct ttctgcctgt gcctgcagac
     7681 accattttta ttacgggaga agagttgacc gcaaaaagct gctggaggct cagaaaatgc
     7741 gtggcatgtg tctcccagga accggaaatc ggcaaaggaa aggtatgcga tattcttgcg
     7801 gctccctttc agtaccggag caacagaagc ctctcttttg agctgaaaga ggcacagcag
     7861 ctttttgaat gctttctgct tcctgcggac acattacaga aagatatgga agtgctgtca
     7921 ggcggcgaaa aacagcgggt cgcgttaacc gccgccctgc tgctgaaacg gaagctgctc
     7981 cttctggatg aagttgccgc cgcccttgat gcccgttcca aagaagcggt gcgggcttat
     8041 cttgcctctc tgaaaaacac cgccattctg tctgtctctc atgatgtcgg ggatttcctg
     8101 ctgtcttcac gactcgaatg gatggaagac ctgcagaaag gaggcggcaa tgcccgtgac
     8161 tgatattgat gtcgtccgcc tggcagccgg gtacggactc ctgctgatcc cgctgggtat
     8221 ttttctctgg catcgggtgc cgctcatcgg gactacgctg gtcagcatcg tgcgcatgac
     8281 cgtacaactc cttttcatcg gtttttatct gcagatactt ttcgatttaa ataatatctg
     8341 gctgaatatc atgtggctgg cggtcatgat tgccgtggcg gatgcgtcca tcctccgcgg
     8401 atgcgattta aacttccgga tctttgcaat tcctgtcttt gtggcgctgc tgctcggtat
     8461 cctgctcccc gtttttattt ttctcaccgt aatcctttat ccggcgcctg ctttggatgc
     8521 acgctttctg gtacccattt tcggcatggt tctggggaac tcgctgcggg ctgttattgt
     8581 agggttgcag cacttctatg acgcactgcg gcgccgtgaa aaggaatatt tcttttttct
     8641 gagcgccggg gcgggcaggc aggaagcgct tatgcctttt ttgaaggaaa cgctccaggt
     8701 gtccatggca ccgaccctgg ccaatatggc gactatggga ttggttgcac tgcccggaat
     8761 gatgacaggc gtcatgatcg gtggaatcag ccctgcagcg gcaatcaaat accagatagc
     8821 gattatgatc gctatcctct ccggcacctc tcttgccgca acgctggcag tttatttcag
     8881 cctgagaaaa agcctgacgc cctgggggag cctcaatcgg agtgtgttta cccgcctgaa
     8941 tcagtagggc gagccggatt ccggctctgc ggtataaaaa gcgtcgatag ccttcaatat
     9001 ctgatcgcgg ggtgccacat tgggcgtgac ccgcagaccg gtaagccgtt caatatcctc
     9061 tatgacaaga agattgcccg ggttttccat caccagcacc agttccttgc ccagcacctg
     9121 tctgggaata cagcagcgcg tttttgccag tcgctcagga accagacgca tagccttcgg
     9181 atccagccct gccgcagaga tggaaacaag cgggcactgg tgcacaacgg aaatgatttc
     9241 tgccagagag gcaggtgtca taaagcccat cttgacaagc acggcgacaa aatcagactc
     9301 aggatgaagt ttctgctcag atatggcatc ggacaattga tccgttgtaa tgcatttcag
     9361 tgccaccagc acttccccca gattccgctg cttctgtctg ggcagggaat tgatcacttc
     9421 gcggacaggc agcgcgccac gagcagaatc tgctgaggga gccactgtct cgacctcgcc
     9481 cgttacgtca gcaggaagcc gtttcatggc acgcagttca gcacgtgcat cttcggcatc
     9541 acggagagca tcggcaagct gacgggcaag ccctgccttg gtgcctttcg aaagttcttc
     9601 gtccagccgt tcctgcagtt gtgcggtttt gcgcttttcc tcttccagag cgctgcgcat
     9661 attttctatt tcttcgggag atgccccctg ctccagttcc acatggaggt ctttcacttt
     9721 attattgaga tcttgaactt cctgcttcaa ttgctcattg attttttgaa gcgcttcttt
     9781 ctcagactca aaactgttct gagcgtccag tgattttttg gcggacgctt ccagtgtctg
     9841 acgcatttct tcagaactca gcaactgcgc ctgagtccgg gacagcgcct cgctcttttc
     9901 atccagttct tttttaagct gttgctgctg cctttcgaga tccgtaaatt tcgttttcca
     9961 ttcagcagca ccgcgttccg ccgtttctgt atcttcggca atggtctgaa gcgcctgacg
    10021 cgcatcggcg gcattgcggg cagcttcttc tttttcttcc gtcaaatcgg cgattttatc
    10081 ctgtgccgca gcgagcgcgc tttccagcgt ttcggtccgt tctttatggg catttagttc
    10141 tccgacaagc gtggcaaccc gcgcttcggc atctccgctt tcagacaaca gtttctcaag
    10201 tttatcttcc gccagctcgg cacgccgttc cgccgtatcc agtgcggttt cccgatcgcc
    10261 cagctcgctt tgaagaaaag aaatttgaga ctgctgttcc gatagctgag ccgacacctt
    10321 ttccgccgat tcctgcacct gagccagata ctcgcgtgct gctgccgtcg ctgctttttc
    10381 acgctccact tcctgctgca gcccaacaac aatgccttcc tgttccgcgt gcgtttttgc
    10441 agcttcgctc agtcgctgct gaaaaccgtc cagggcttca cgggctgcat tcagttcgct
    10501 gttcaggcgg ttgcactccg cttccagttc ctgctggcga ttttcaccgg cagccgtgtc
    10561 cgaatccata cgggctgccg catcgcgcaa cacgtccatt tccgcaagaa gcgctgcttt
    10621 ctcccgggca gccgcaacaa gctgttcctc catctccgct ttttcctcag caaaccggcc
    10681 ggctgcagct gcctgttcct gcagctggtg cgccacatct tgtttacttt cttcaaaagc
    10741 agctgcctgc tgtttcaagg catcatagtg gctttgcagg gcactcatct ccgcctgctt
    10801 ttcttccatc tcctgacgca gttccaaatc ggcctgctcc agtgcgtgga tgcgctcctg
    10861 taccgatgcc agatcgcggc tccggctgct cacatcgtcc tgcagctgac ggtttcgttc
    10921 ttctgcgccc gacaacgctt cttctttatc tgcgaggaga cgctcaagcg cctgaatctg
    10981 cgcctgattt tcctgatcgc tgccgctgtt ctcctccagt tgtttgcgca attcgttgaa
    11041 gcgatcttcg gaggactgga gtgctttatt tttgcgttcc agctgcagct ccagatcttc
    11101 ggcgcgctgt acagccgaag tgcgttccgc cgccatgcct tcgatctttt cctgaaggcg
    11161 aagaatacgg cggcgtgcta caccgatggt ctcatttctt ttctgcagat gttcttccag
    11221 ccctttaata tagtgctgga ctttctcgtc ccctccggct gaaacctgcg cctcaagggc
    11281 agcgattttc cgatgttgct cagcaagctg tttctctttg tggtgaactt cttcttccag
    11341 atccaggata cgtttatgca gtccgtcgcc gccttccggc agaccctgca cctgagatgc
    11401 cagcatttca taagcactcg catgttttgc aaacacggag gcctgctgct cataggcgct
    11461 ttccagttga gcttccagcg cctgaaacgt ggtttttagt tgtgtaagtt gtgatactag
    11521 agcctgatgg gattcattac agcgtcccgc tccctctttc accgaatcca gaaaagcgat
    11581 tagctcgttg tttgctgctt tgtcttgcat aatgttttcc aatacccttc aagatcggaa
    11641 catattacac ctgttcacaa cgccacttaa gtataaatta tttactgatt tatatcaaga
    11701 tatgaaagcc accagaaata tgaagaacgc aacggatgcc tggaaaaaat tgataaaaat
    11761 acatgctgcg caaaaggatt atgccaagaa gtttctgtac attaatttga accgacgggg
    11821 ccttcgttcg gttctctcct ggtgaatact aaaaacccgc ctaaaggcct aattaaaggg
    11881 aggacaaatg ttgaccggta aagaatatgg aagttttttt acaacagatt ttcaagaaac
    11941 ctcttgacat aacttcggat attaaatcat tatattggaa caggagacct ctgcaatgcg
    12001 ttcaaccttt ttattttgtt tatcattggc gatgggcgct gccgcgtggg gcgatgcgcc
    12061 gtcgttcatg atggaaaacg acaggataaa gattgtattg gatgccgcca cgggtggttt
    12121 tgcttcgata tatgacaagc aaaaggcgga ttcaaggctg aaatgcaatt attgaattga
    12181 ataagacttc ggcagg
//
LOCUS       DAOPWB010000095        11835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5541_length_11835_cov_5.128353, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000095 DAOPWB010000000
VERSION     DAOPWB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5541_length_11835_cov_5.128353"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKN92_09195"
     CDS             <1..358
                     /locus_tag="PKN92_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNZ49007.1"
                     /translation="STVEIPGDLQWVQIDLEEEKEIYALVVWHFHEGKRVYFDIVVQA
                     SNDKEFSDGVTTIYNNDHDNSSGLGVGEDKEYQENYQGRLMDAKGVSARYVRLYSSGN
                     TADDFNHYVEVEVYGK"
     gene            483..1388
                     /locus_tag="PKN92_09200"
     CDS             483..1388
                     /locus_tag="PKN92_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49008.1"
                     /translation="MPVISRQVSWLPVTLIGAAAVLWSVAVFAALTAEPEMLFFSGHS
                     DTHALQVFAENQPVSSLSVDHWKFYTDSSSYPHMIQVEFKDGRIYVKTTERSEDGSYL
                     LVVATSLGELRIPVRITLPSASPTAYADTGTTGVKLRSTEKRIPLLELESFYLEGTRL
                     YIDMGCAPEYSACWWINNEIVSKDSRLLISLDQPGPLHVVYEERCGNQVVAQAEARSE
                     VLPRGDETVPFSTKINARNTFKAPLGYAQHKWYVNNEFLSELENFRHTFLKAGAYQVK
                     VNVAAPDDTSLPSRRTILYEVTVAP"
     gene            complement(1481..2797)
                     /locus_tag="PKN92_09205"
     CDS             complement(1481..2797)
                     /locus_tag="PKN92_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ49009.1"
                     /translation="MTNLQPGLIKKQFTSGLTGYHYLVLMVACLGWAFDTMDQWLFVF
                     VKQHALRELLGPLMSDAEVTRHVGYVQSCMLIGWATGGLIFGMIGDRLGRTRTMAITI
                     LMYAGFTGLSGLAQNWQQFAILRFLMGLGVGGEFAAGAALVAETFPEHARAVALGIVQ
                     AASAFGNIAAAGINFAMASILPPDQAWRWIFAIGIIPGLLVFVIIMFIKEPDKWKEAN
                     ARAKQLKAEGIKQTGSLLALFTERGVRKNTLVGVGLATIGVIGFWGISTWTPELLRSI
                     LDPTLPALTKERYVSLAGMAQNLGGFFGALCFAVVANRYGRRAAFALALIGSSIIIPV
                     TFHYTTAFTLALAFFFAMGFILLFFLSGFAIYFPELFPTRLRATGTGFCYNVARYLAA
                     FFPAFFGILAGRFGIGNAAMILSAFFILGLFIVYVAPETKGKPLPE"
     gene            complement(2859..3551)
                     /locus_tag="PKN92_09210"
     CDS             complement(2859..3551)
                     /locus_tag="PKN92_09210"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNZ49010.1"
                     /translation="MNDTAADSTITDPPAKLLRNIILLGHFFCLVLAVVYALTWFLYG
                     KPYDQAWRIIFLSLTLGRAAAVGACAKMGFGLSFIFYQIVFVDLIFILYLYPLFAKGF
                     RQIVRIRYIGGYLQSIHEVALGYKSTVAPYGAIGLTLFVACPLWSTGPLIGTLVGYII
                     GLPAIIVLSAVTLGNILATVFWIWLYDWLDNWSSMLSLILVIFVFILSTAGLVLAGIK
                     NFKKGLSDTVGK"
     gene            complement(3680..4165)
                     /locus_tag="PKN92_09215"
     CDS             complement(3680..4165)
                     /locus_tag="PKN92_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNZ49011.1"
                     /translation="MNSFRKRTYTGVQEFFGDLCFLLARFKQVRKIMSGKGLDPAFRE
                     RLMLAVTRVNDCRHCIRFHTKAALDEGMAQEEIRQFLAGEYDHCPETQMEAVLFAERW
                     AHAGGQAEAEDLKNLISSYGAETAQNIQIALRIIRAGNLTGNSIDALLFKISLGRWGN
                     G"
     gene            complement(4266..5321)
                     /locus_tag="PKN92_09220"
     CDS             complement(4266..5321)
                     /locus_tag="PKN92_09220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ49012.1"
                     /translation="MIKFFSAHASMMIAMLCILAGAPGTAAAKECNRITGESLVLVKE
                     EPASLIFKNIRPDTVTVRNTYLPGKENTVTYKEGVDYLLDPEAGTLCRIAGSSIPDFS
                     KNMLYGQANFSHADFPGFGNTDFFVYVDYESEDSSSLFMPTEQGDLLPRTKEKLTQGQ
                     PLKLIAFGDSITAGGDASEVKLQFPERYAAYLREKYPNADIRLENGATGGDSTADGLV
                     RLEEKVLSRTPDLVLVAFGMNDHNKRGINPENFHANLSDIVSSIREKTGADIILLSTF
                     PPNPLWRHGSHCMEQYAQITKQVAREQNCAYADVYSVWMKVLARKDFSSLLGNNVNHP
                     SDFGHWLYCEVLKSVSF"
     gene            complement(5381..7276)
                     /gene="typA"
                     /locus_tag="PKN92_09225"
     CDS             complement(5381..7276)
                     /gene="typA"
                     /locus_tag="PKN92_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNZ49013.1"
                     /translation="MHPISSIRNVAIIAHIDHGKTTLIDSIFRAAHMFRENARVAQRF
                     MDNNELERERGITIRSKHCTVEWKNYLINIIDTPGHADFSGEVERVLSMVDSVLLLVD
                     ANEGPMPQTRYVLMRALRLGLRPIVIINKVDRPNAEPLRALDATFDLFLTLGATEEQC
                     DFITLYGSGLNGWLSRNPEGSPEEGMEALFETIVTHVPPPKGKEDAPFLMQVNAIGWN
                     EHIGRTGTGRILQGRLTKGMEITRWTTKRKEVKRLALDTDTFEWSVTGSSQEKAVYLW
                     ATQGLSTVSIHEVGTGDIVTLAGPKELNIGDTLAAVELENPALPPLDIEEPTVSMMFL
                     INSGPFSGREGKAVTQRQLKERLMREIRVNVSLRVEDTGRMDALKVSGRGELHLSILI
                     EEMRREGMEFCVSAPEVIIQTTEEGSILEPVEEVVIDVMREHQGIVIEKVSERKGELL
                     SMQIGTGEMIRMEFRVPTRGLIGYRGEFLTDTRGLGIMASRFSGYALWYGNIPGRNRG
                     SMVSMDTGYATGYSLENLQQRGTLFISPMTEVYTGMIIGENARPGDLPCNPTKRKQLT
                     NHRSATKEQTVTFDVPRTLSLEEALEWIGKDELVEITPSAIRLRKTVLDFEERKKIER
                     HLSAVEG"
     gene            complement(7501..8664)
                     /locus_tag="PKN92_09230"
     CDS             complement(7501..8664)
                     /locus_tag="PKN92_09230"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HNZ49014.1"
                     /translation="MKRTSFPVFMFILCFGFFLQNAHPEEQEWSIYIGTYTHGESRGI
                     YHLTMNRETGEMGLPSLAAETENPSFLALHPDGTVLYSVGNAADGENGTKGYINAFSI
                     DRKTGKLDFINRQEVRGTEPCHVAVSRGGNYAVTANYGNGSVTCFPLEPDGKVTALCD
                     YRMHSGSGINPSRQEAPHAHSVTFNALGNRLYVADLGIDRVLVYRFDAQRGLIDSEEE
                     ASLAVDAGSGPRHFAFHPQKTVAYIVNELANTVTVCQYNASSGKLSFLQTVPTLPPDF
                     KGESTTAEIRVHPSGRFVYASNRGHDSIAVFSVDPISGKLAPSGFAFTKGKNPRHFSL
                     SPDGRFCIVGNALSDTLCLFTVNGSTGALEATEQEVAVPYPVCILFDAKANKQ"
     gene            complement(8695..>11835)
                     /locus_tag="PKN92_09235"
     CDS             complement(8695..>11835)
                     /locus_tag="PKN92_09235"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNZ49015.1"
                     /translation="AAFLAMPTHADYRAGQCYDTAWDAFQNPDNSFRGKPFWSWNGKL
                     RKEELLRQIEVLEEMGFGGFFMHSRTGLATEYLGEEWFDLINVCADEAEKRNLEAWLY
                     DEDRWPSGSAGGKATEDPRYQMKYLLMEIMDAAEFKWEERFIAAFSCTLEGINYSALK
                     RLDKESSAPGEGRTVLAFSVKPMDSSSFYNGNTYLDTMNAEATQHFIEITHDAYKKHC
                     GDRIGTSIKGIFTDEPHRGMVMCHKVDQGGLSASERCTPWTPALPAAFQKEYGYDLLD
                     HLPALFLRPEGQRIVRVKWEYMELIQKLFLENFAKPIQKWCRENQMILTGHALHEDTL
                     GAQAVPCGSMMRFYEYMDYPGVDVLTEGNKNYWIVKQLSSVARQLDKKWLLSELYGCT
                     GWQFNFASHKAVGDWQSLLGINLRCHHLSWYTMEGEAKRDFPASIFYQSAWYPEYKEV
                     ETYFSRVQAFMSRGEPECNILVLNPVESAWAQIYTGWATWLSATAPEVVALDQKYQQL
                     LLWLLSAQMDFDIGDEDMLARHGKIEKESTPRLTIGKGKYGLVIVSGMETIRDTTLAL
                     LEQFQAAGGSILFADLPPAYVNADASQRAIDLASAALRAPFEREPILEICKKSAGQSV
                     ALHHESDPDICTRIFCQSRTDEDLRFIMALNTDRTQAVEGVQMSVPGKGVVEKWDAMT
                     GQRTRIPADFSGGMLRFSLDFPAGGEKLLLIRPDRDPGVPLEKTYKELKRTAFEGACP
                     YELSEPNVCVLDRASCSIDGKAFGTPMEILKVDQYVRDHFGLARRGGEMVQPWFTGMK
                     DRVIQGRVTLRYDFEAEIIPETLYLVLEQPDVFQVLINGTELSVKEDVGWWVDTCLRK
                     ITLPVNLLKKGENLIEVTVDFHEEINLEALYLLGNFGVKLDRIRRILTDLPDTLAFSD
                     VTEQGLPFYSGAIDYLLTRAQPADEPVGDAFISLPSFEGACAKLCQGKEDDLIAWAPY
                     EAPLETEQGGDQKIRLRVLLTRRNTFGPLHQTTLKTAAYGPGNWVTTGTGFTEHYNLY
                     PAGLLQAPELIVREAI"
BASE COUNT         2964 a         3010 c         2887 g         2974 t
ORIGIN      
        1 cagcacggtt gaaattcccg gcgaccttca gtgggtacag attgatcttg aagaagaaaa
       61 agaaatctat gcgcttgtcg tatggcactt ccacgaaggc aaacgagttt attttgatat
      121 cgtcgttcag gcctccaatg ataaagagtt ttccgacggt gtaaccacga tttataacaa
      181 cgaccacgat aattcttccg gtcttggtgt tggcgaagac aaagaatatc aggaaaacta
      241 tcagggtcgt ctgatggatg ccaagggtgt ttccgcacgc tatgtgcgcc tctactccag
      301 cggcaatacc gctgatgact tcaaccacta tgtggaagtc gaagtgtacg gcaaataagc
      361 cccttccctg ttttttctgc ccgcattgtc ttattggcaa tgcgggcttt ttcatggaac
      421 gtgaatgcac tttggggaat taaataaata taaatgaatg tctttgtctg aagggaaaaa
      481 tcatgccggt catatcaaga caagtatcat ggctgcctgt tacgctcatc ggtgcggcag
      541 cggtattgtg gtctgttgct gtttttgcgg cgctcacagc tgaacctgaa atgcttttct
      601 tctccggtca cagtgacacc cacgcacttc aggtctttgc ggagaatcag cctgtctcct
      661 ccttatcggt ggatcactgg aaattttata cagactccag ttcatacccg catatgattc
      721 aagtggagtt caaagacggc aggatatacg tgaagactac cgaacgttca gaggacggca
      781 gttatcttct tgtcgtagcg acgagtctgg gtgaacttcg catccctgtt cgaattacgc
      841 tgccctcggc ttctccgact gcttacgctg ataccggcac aaccggagtg aagctgcgtt
      901 caacggagaa gcgtatcccc cttctggaac tggaatcctt ttatctggag gggacccgcc
      961 tctacataga tatgggttgt gctcctgaat atagcgcctg ctggtggatt aataatgaga
     1021 tcgtctcaaa agacagtcgc ttgctgatat ctcttgacca gccgggtcca ttgcatgttg
     1081 tctatgaaga gcgctgcgga aatcaggtgg ttgcacaggc ggaagcccgc tctgaagttc
     1141 ttccccgtgg tgacgaaacg gtgccgtttt ccacaaaaat taatgcacgt aatacattta
     1201 aagcaccgct ggggtatgcc cagcacaaat ggtatgtgaa caatgagttc ctgtcggaat
     1261 tagaaaattt taggcacaca tttttgaaag ccggtgctta tcaggttaaa gttaatgttg
     1321 cagcgccgga tgatacctca ttgccttcca gacgtacgat actctacgaa gtaacagtcg
     1381 ctccttgaat ggaatccgaa tagaggtaac taagaaaagg gctgcaggac aggtgatcca
     1441 tgcagccctt taatgctttc agtacgtaga caggcttcga ttattcaggt aagggctttc
     1501 ctttggtttc aggcgcaaca taaacgataa aaagaccaag gataaagaaa gcggagagta
     1561 tcattgcggc atttccaatg ccgaagcgcc ctgccagaat cccgaaaaag gcggggaaaa
     1621 aggctgccag atagcgcgcc acgttgtagc agaagccggt tcctgtagcg cgcagacgcg
     1681 tcgggaaaag ctccgggaaa taaatagcaa atccgctcag gaagaaaagc aggataaatc
     1741 ccatggcaaa gaaaaaagca agtgccaatg taaaggctgt agtgtaatga aaagtgaccg
     1801 ggatgattat ggaagaaccg attaacgcca gtgcaaatgc cgcccgacgt ccataacgat
     1861 tggctacaac ggcaaagcat agagcaccga agaagccgcc gaggttctgt gccattcccg
     1921 ccaggctgac ataacgttct ttggttaggg cgggcaaggt gggatccaga atactacgga
     1981 gcagttccgg ggtccaggtg ctgatgcccc agaaaccaat gacgccaatg gtagcaaggc
     2041 cgacgcccac caaggtgttt tttcgaacac ctctttctgt gaatagagca agaaggctcc
     2101 ccgtctgctt gatcccttcc gctttcagct gcttggctct tgcattcgcc tctttccatt
     2161 tatccggctc tttaatgaac atgataatga cgaacaccag aagtccggga ataataccaa
     2221 tagcaaagat ccagcgccag gcctgatcag ggggaagaat ggaagccatg gcgaaattaa
     2281 ttcctgctgc agcgatgttg ccgaatgcgg aagctgcctg tacgatacca agggctacag
     2341 cccgggcgtg ttccggaaag gtttcagcca caagcgcggc accggctgca aattctccgc
     2401 cgacgccgag acccatcaga aagcgaagaa tggcaaactg ctgccagttc tgcgccagcc
     2461 cgctcagacc ggtaaagcct gcatacatga ggatggttat tgccatggtt cgggttcgcc
     2521 ccagccgatc ccctatcatg ccgaagatca gaccgcccgt cgcccatcca atgagcatac
     2581 aggattgcac ataaccgaca tggcgcgtca cttcggcgtc gctcatcagt ggaccgagca
     2641 gctcgcgcag cgcatgctgc ttgacaaaaa caaagagcca ttgatccatg gtgtcaaagg
     2701 cccagcctag gcaggcaacc attaaaacga gataatggta tccggtcagc ccggaagtaa
     2761 attgcttctt tatgagaccc ggttgaagat tagtcattta cttctccctt cctttttaaa
     2821 cgcccgcaat cagccggggt atcagaataa aaccttgttt atttaccaac tgtgtcagaa
     2881 agcccttttt taaaattttt gatgccggcc aggaccaggc ctgccgtgga taaaatgaaa
     2941 acaaaaataa ctagaataag cgacagcatg ctgctccagt tatccagcca gtcataaagc
     3001 caaatccaga aaacggttgc gaggatattt ccgagcgtca cagcggacag tacaataatg
     3061 gcgggaagac cgataatgta gccgacaagt gttcctatca ggggaccggt gctccataaa
     3121 gggcaggcaa caaaaagcgt gagacctatg gcaccataag gcgcaactgt agatttatat
     3181 cccaatgcca cttcatggat agattggaga tagccgccaa tataacgtat cctgacaatc
     3241 tgcctgaatc ctttggcgaa caaaggatac agatagagaa taaaaattag gtctacaaat
     3301 acaatctgat aaaatatgaa ggaaagacca aagcccattt tggcgcaggc ccctacggca
     3361 gcagcacgcc ccagagtcag ggagagaaaa ataattcgcc aggcctgatc ataaggtttg
     3421 ccatacaaaa accaggtaag agcatagaca actgccagaa caaggcaaaa aaagtgcccg
     3481 agcagaatta tattgcgcag cagtttcgcc ggcggatctg tgattgtgct gtcagcagct
     3541 gtgtcattca tggtggttta atcaatcagt tttttattgc tcgctctact gtcccgtcag
     3601 gaccagcaat tacacttgta tcacatatcc agtgagctag acaaaagagt gcagtttttt
     3661 tgcagtcggt ccggaagggc tatccatttc cccaacgtcc caatgaaatt ttaaataaca
     3721 aagcatcaat gctgtttcct gtcagatttc cagcccgaat gattcggagg gcaatttgta
     3781 tgttttgtgc agtttcggca ccataagaag atataagatt tttcagatct tcagcttcgg
     3841 cttgcccgcc ggcgtgcgcc catctttctg caaaaagaac agcttccatc tgtgtttccg
     3901 gacagtgatc atattcgcct gccagaaact gcctgatttc ttcctgtgcc attccttcgt
     3961 ccagagcagc ttttgtatga aagcgaatgc aatggcggca gtcattcaca cgggtaacag
     4021 caagcatgag gcgttcgcga aacgcaggat ccaagccttt gccactcatt attttccgga
     4081 cttgcttaaa tcgggcaagc agaaaacaaa gatctccaaa aaattcctgc actccggtat
     4141 aggttcgttt tcgaaatgaa ttcatcaggc ttctttccaa agaaaaaacg gcatgcttta
     4201 aaagggcatg ccgtcgtatt gtaccataat caacgttagt acaagttgct gtacatggaa
     4261 agtgtttaaa aagaaacgct ttttagaact tcgcaataaa gccagtgtcc gaaatcgctt
     4321 gggtgattta cattattgcc aaggagactt gaaaagtctt ttcttgctag cactttcatc
     4381 cagacggagt aaacgtccgc ataggcgcag ttctgctccc gggcaacctg ctttgttatc
     4441 tgggcatact gttccataca gtgggatccg tgcctccata agggattggg cgggaaggtg
     4501 gaaagaagaa taatatcagc ccctgttttc tcccgtatgg aggaaacaat gtcgctcagg
     4561 ttggcatgaa agttttccgg attgatccct cttttattgt gatcattcat gccgaaggcg
     4621 accagcacaa gatcaggtgt gcggctgagc accttttctt ccagacgaac caaaccatcg
     4681 gccgtgctgt ccccgcctgt tgctccgttc tccagacgga tgtcggcatt gggatatttt
     4741 tctctcagat aggctgcata gcgttcaggg aattgcagtt ttacctcaga ggcatcaccg
     4801 ccggccgtga tactgtcacc aaaagcaatg agttttaacg gctgcccctg cgtcagtttt
     4861 tcttttgtcc gtggcaacag atccccctgc tccgtgggca taaaaagcga agagctgtct
     4921 tctgattcat agtctacata aacaaaaaag tcagtattgc caaagcccgg aaagtcggca
     4981 tgactgaagt tcgcctgccc gtacaacatg tttttcgaaa aatcggggat ggaagaccct
     5041 gcgatgcggc acagtgtacc cgcctcagga tcaagcagat agtccacgcc ttctttataa
     5101 gtgacggtat tttcctttcc cggaaggtaa gtatttctta ctgttacagt atccggccgg
     5161 atgtttttaa atatcaggga ggcaggctct tctttaacca gaaccaggct ctcccctgtt
     5221 atccggttac attcctttgc agcagcagtt cccggcgctc ccgcaaggat gcaaagcatt
     5281 gcgatcatca tagaggcatg agcagaaaaa aacttgatca taaaactgtt ctccaatggt
     5341 gtcgataaaa agtgcctgcg cccgacaggg ctttctacga ctatccttcc acagcactga
     5401 gatgccgttc aattttcttt cgttcctcga agtcgagtac tgtttttcgg agtctgattg
     5461 ccgaaggggt tatttcaacc agctcatctt tgccgatcca ttccaatgct tcctccagac
     5521 ttaatgtacg ggggacatca aaggtaaccg tctgttcctt tgttgcggaa cgatgatttg
     5581 tcaactgctt gcgtttggtt ggattgcacg gcagatcgcc gggccgtgcg ttttccccga
     5641 taatcatccc ggtatacacc tctgtcattg gagagatgaa taaagtacct cgctgctgca
     5701 ggttttcaag gctgtacccg gtcgcatacc ctgtatccat actgaccatg gagcctctgt
     5761 ttcgtcccgg aatatttcca taccagagtg catagccgga aaagcgcgat gccatgatgc
     5821 cgagtccgcg ggtgtcagta agaaattctc cccggtagcc gatgagcccg cgggttggga
     5881 cccgaaattc catcctgatc atttcccccg tgccgatttg catggatagc agttcccctt
     5941 tgcgttcgga tactttttct atgacaatcc cctgatgctc ccgcatgaca tctattacca
     6001 cctcttctac aggttcaaga atgctgcctt cttctgttgt ctgaatgatt acttcggggg
     6061 cagatacgca gaattccatg ccttctcttc gcatttcttc aataagaatg gataagtgga
     6121 gttcgccacg gccggagact ttcaaggcat ccatgcgccc ggtgtcctcc actctcaggg
     6181 aaacattgac acgtatctca cgcatcagac gctctttgag ctggcgctgt gtaactgctt
     6241 ttccttccct gcccgaaaaa ggaccgctgt tgattaaaaa catcatggaa acggtcggct
     6301 cttcgatatc aagcggcgga agtgcaggat tctccagctc gacagcagca agggtgtcgc
     6361 caatattcaa ttctttggga ccggcgagcg tcacaatatc tccggtgccg acttcatgga
     6421 tcgagacagt ggataaaccc tgggttgccc acagatacac agccttttcc tggctgctgc
     6481 ctgttacact ccattcaaag gtatcggtgt ccagagccag acgtttaact tcttttcttt
     6541 tcgtggtcca tcgggttatc tccatacctt tagtgagacg tccctgaagg attcttccgg
     6601 tgccggttcg gcctatgtgc tcattccatc cgatcgcgtt cacctgcatc aggaaggggg
     6661 catcttcttt tcctttcgga ggaggaacgt gggttacaat cgtctcaaaa agtgcttcca
     6721 tcccttcttc aggcgacccc tccgggttcc gggatagcca cccattcaaa ccggaaccat
     6781 acagcgtaat aaagtcgcac tgttcttctg tggcaccaag cgtgaggaac agatcaaagg
     6841 tggcatcaag agcacgcagc ggttccgcat tcggccggtc gactttattg ataatcacga
     6901 tgggacgcag cccgaggcgc agtgcccgca tcagtacata tcttgtttgc ggcatagggc
     6961 cttcgttggc gtccacaaga agtagaactg agtccaccat ggaaagcaca cgctctactt
     7021 ctccggaaaa gtcggcgtgt ccgggcgtat cgatgatatt gataaggtag tttttccatt
     7081 ccacagtgca atgctttgaa cggatcgtaa tgcctcgttc ccgctccaac tcgttgttgt
     7141 ccatgaaacg ctgtgcaacc cgtgcatttt cacgaaacat gtgggcagcg cggaaaatgc
     7201 tgtcaataag cgtagttttc ccgtggtcga tatgggcaat tatagcgaca tttctgatag
     7261 aactgatggg atgcatggat aaaagactcc ggcgcctgag aaaggctgtg aacgcaaacc
     7321 ccgccctatt tcgatggagg cgtcctgaat aagtggtgga aaacgcccgg gattcgcgat
     7381 aagttgaggg ggaacggtga atattatgcg ctttttaggg gtagaagcac aatatttttt
     7441 tccgggagcg gagaaatgga ggcgcaactt tgaaagtcag ttttctcttt cccgggaatt
     7501 tcactgctta tttgcctttg cgtcgaagag aatgcatacc ggatatggga cagccacctc
     7561 ctgttcagtg gcttccagag cgccggtgga accattcaca gtgaataggc aaagggtatc
     7621 gctcagcgcg ttccccacaa tacagaaacg cccatccgga gaaagtgaaa aatggcgcgg
     7681 gtttttcccc tttgtaaagg caaaaccgga aggagccagt tttccgctga ttgggtccac
     7741 tgaaaaaaca gcgatgctgt cgtgtcctct gttggaggcg tacacaaatc tcccggaggg
     7801 atgcaccctg atctccgcag tagtgctctc ccctttaaaa tcaggtggta acgtagggac
     7861 tgtctgcagg aaactgagtt ttccgctgga agcattatat tgacataccg ttactgtgtt
     7921 cgcaagttca ttaacaatat atgccaccgt tttttgcggg tggaaagcaa agtgtcttgg
     7981 cccggatccg gcatcaacag caagagaggc ttcttcttca gaatcaatta aaccacgctg
     8041 tgcatcaaaa cgatagacca gcactctgtc tataccgaga tcggcgacat agagccggtt
     8101 gcccagcgca ttgaaagtga cagagtgggc atggggcgct tcctggcgtg aagggtttat
     8161 gcccgatccg ctgtgcatcc tgtaatcaca cagtgcagtc acttttccgt ctggttccag
     8221 cgggaaacag gtcacactgc cattcccgta attggcggta actgcatagt ttcctccacg
     8281 ggacacagca acatggcaag gctctgttcc tctgacttcc tgccggttaa tgaagtctag
     8341 ttttcctgtt ttgcggtcaa tggaaaaggc attgatatac ccttttgttc cgttttctcc
     8401 atcagcggca tttccgacac tgtacagaac cgttccgtcg ggatgcaatg ccagaaagga
     8461 gggattctct gtttcagctg caagtgacgg aaggcccatc tctccggttt ccctgttcat
     8521 cgtcaggtga tagatgcctc tgctctcacc gtgtgtgtag gtgccgatat agatagacca
     8581 ctcctgctcc tcaggatgtg cattttgcaa aaaaaaaccg aagcacagta taaacataaa
     8641 aacaggaaaa gaagtacgtt tcatctgtca gttgctcccc ggcaaggcca ttgcttatat
     8701 cgcttcgcgc acgatcagtt caggtgcctg caacagcccg gcaggataca gattgtagtg
     8761 ttctgtaaaa cctgttcctg tagtgaccca gtttccggga ccgtatgctg cagttttcag
     8821 tgttgtctga tggagaggac cgaaggtgtt gcgtcttgtc aatagtaccc gcagcctgat
     8881 tttctggtct ccgccctgct cagtttcaag aggagcctca tagggcgccc aggcaataag
     8941 atcatcttct tttccctggc agagtttggc gcacgcccct tcaaaagaag gaaggctgat
     9001 aaaggcgtca cccaccggtt catcagcagg ttgtgcgcga gtaagcagat aatcaatggc
     9061 accggaatag aatggaagcc cctgttccgt aacatcactg aacgccaatg tgtcgggcag
     9121 atctgttaaa atccgccgga tcctgtccag tttaacacca aagttgccaa gcagatataa
     9181 ggcttcgaga ttgatttctt catgaaaatc cacagtaact tcaattagat tttccccttt
     9241 tttcagaagg tttactggaa gcgtaatttt tctcaaacag gtgtccaccc accagccgac
     9301 gtcttctttc acagaaagtt ctgtgccgtt aatgagaacc tgaaagacgt cgggctgttc
     9361 caggaccaga taaagggtct ctggaataat ctctgcctca aagtcatagc gcaaagtgac
     9421 cctgccttga ataacccggt ctttcatccc ggtgaaccag ggctgcacca tttctccgcc
     9481 ccgtcgtgcc agaccgaaat gatcacgcac atactggtcg actttcaaaa tttccatggg
     9541 ggtgccaaac gcttttccgt ctatgctaca ggaggcgcga tccagtacac acacgttcgg
     9601 ttcactcaac tcatagggac aggcgccttc aaaggctgtc cgtttcaatt ctttgtaggt
     9661 cttttccaga ggaacgccgg gatctctatc cggtctgata aggaggagct tttctcctcc
     9721 tgctggaaag tccagcgaaa atcgaagcat tcctccggag aaatcggctg ggattctggt
     9781 tcgttggcct gtcatcgcat cccatttttc aacaacgcct tttcccggta cggacatttg
     9841 cacgccctca accgcctgtg tccggtctgt gttcaacgcc ataataaatc ggagatcttc
     9901 atctgtgcgg ctttggcaga aaatacgggt gcagatatcg gggtcggact catggtggag
     9961 tgcgacgctt tgccctgccg actttttgca gatttccaga atgggttccc gttcaaaagg
    10021 ggctcgcaag gctgcagaag ccaggtcgat ggcgcgctgc gaagcatcag cattcacata
    10081 ggctggtggc aggtcggcaa aaaggatgga tccccccgct gcctgaaatt gctccagcag
    10141 cgcaagtgta gtgtctcgga tcgtttccat tcccgaaaca atcacaagcc cgtacttacc
    10201 ttttccaatc gtgagtcggg gggtgctttc tttttcaatt tttccatgcc gcgccagcat
    10261 atcttcatct cctatgtcga aatccatctg tgccgagagc aaccatagca ataattgctg
    10321 atatttctga tccagcgcga cgacttccgg agcggtggca gatagccagg ttgcccagcc
    10381 tgtgtaaatc tgtgcccagg cgctttcaac gggattcagc acaaggatat tgcattcagg
    10441 ctcgccccgg gacatgaatg cctgcacgcg tgaaaagtat gtttccactt ctttatattc
    10501 ggggtaccag gcgctctgat aaaaaatact ggcaggaaaa tcgcgctttg cctcgccctc
    10561 catggtatac caggacaaat ggtggcatcg caggttgatc cccagaaggc tttgccaatc
    10621 gcccacagct ttgtgagaag caaagttaaa ctgccagccg gtacacccgt aaagttccga
    10681 gagcagccac ttcttatcca actgccgggc aacggatgac agctgcttga caatccagta
    10741 atttttattg ccttccgtca gaacgtctac tccagggtag tccatatatt cataaaaacg
    10801 catcatggag ccgcaaggta ccgcctgagc gcccagcgtg tcttcatgaa gtgcgtgacc
    10861 cgtcaggatc atctggtttt ctctgcacca cttttgtatg ggttttgcaa agttctccag
    10921 aaatagtttc tgaatcagtt ccatgtattc ccatttgacc cggacaatac gctgcccttc
    10981 cggacggagg aaaagagcag gcaaatgatc cagcagatcg tagccgtact ctttttgaaa
    11041 agcggcgggc agagccggag tccagggcgt gcagcgctcg gacgcactca ggccgccctg
    11101 atccactttg tgacacatca ccatgccacg atgcggttca tccgtgaaaa tgcctttaat
    11161 ggaagtgcca atgcggtcac cgcagtgctt tttataggca tcgtgcgtga tttcaatgaa
    11221 atgctgtgtt gcctcggcgt tcatcgtatc cagataggtg ttcccattgt agaaggaact
    11281 gctgtccatc ggtttgacag aaaaggcaag cactgttctc ccctctccgg gagcagaaga
    11341 ctctttatcc agacgcttca gcgcactgta attaatccct tccagagtgc atgaaaaagc
    11401 ggcgataaaa cgctcttccc atttgaattc ggcggcgtcc attatttcca tgagaagata
    11461 tttcatctga taccggggat cttcagtggc tttaccccct gccgagccgc tgggccagcg
    11521 atcttcatcg tagagccagg cttccagatt tctcttttcc gcttcatcgg cgcaaacatt
    11581 aatcagatca aaccattctt cgcccagata ttctgtggca agaccggtac gtgagtgcat
    11641 gaaaaaaccg ccgaagccca tttcctccag cacttctatc tgccgcagca attcctcttt
    11701 gcggagtttc ccgttccagg accagaacgg cttccccctg aagctgttgt ccggattctg
    11761 aaaggcgtcc caggcggtat cgtagcattg ccctgctctg taatcggcgt gggtcggcat
    11821 cgccaaaaag gcggc
//
LOCUS       DAOPWB010000096        11652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5637_length_11652_cov_4.699491, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000096 DAOPWB010000000
VERSION     DAOPWB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5637_length_11652_cov_4.699491"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKN92_09240"
     CDS             complement(<1..525)
                     /locus_tag="PKN92_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNZ49016.1"
                     /translation="MHSTLFLFVITMLYLFITGYLGYLGYRNTRTATDYMVAGRNAHP
                     AIMALSYGATFISTSAIVGFGGAAGMFGMSVLWLTVLNITVGIFIAFVFIGKRTRAMG
                     HRLNAHTFPELVAKRFGSPFLGLFSSLVIIGFMPLYTSVVLMGAAKFIEVRLSVQYET
                     ALLFFSAIVALYVIM"
     gene            complement(573..746)
                     /locus_tag="PKN92_09245"
     CDS             complement(573..746)
                     /locus_tag="PKN92_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49017.1"
                     /translation="MYLGLADFWVFMAYILCIGAALLCVGYGLINWNRNGSEPTEADL
                     KWAEESDKLSEKL"
     gene            1450..9495
                     /locus_tag="PKN92_09250"
     CDS             1450..9495
                     /locus_tag="PKN92_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNZ49018.1"
                     /translation="MIFRTDLPGKWSRMGTGLTVVFLVLSAITGAFAEAPAAVKEALS
                     ASGVWVDLYQESNPEHQEWAASVCALVPADALLSDASDLTILLSDAYKPEGLAPCADH
                     PYASQPAAALCSGFAAGEDLIITAGSCLADGIALDSYRFIFGYALTAPSSVVLDFSAD
                     QIYTPVEVVAFSDEAGADFALVRVDRPITVAGALPLSSDALEAGQAVFTAAYPSGLPE
                     KLAAASAMSAEAGVGVALAPVGDLSGAPVIVGATGEVAGILSGGAAVSADAPCYGYDP
                     ASGAVSAVGFTASSAFSSFVSGVAAEKQAAEGETPDVPAADFTVDSLIRDGEALLPLH
                     DWVPLFRFSMSYGEEPAPRELEELKYRLINDDPEEREYLVRPDLRQSDILEFGLFSDS
                     FSTDTEGNEDRYGALSTDDPLLFTWDATGAPSGSARGLEYSMNFRGRNVVAGPQIDDV
                     MPDGRVYIVAVRTSPTWRSALSMGVEVTMARMVEPRTGVFIPELDSYSPNFYEGEKLE
                     DSGFYSSSFGVYDPTGPDLGGMSGFESWKDIATPNFFQYTPYLSVPLSEFSRPRWNKP
                     DSLISIATGQFMNIRTLMPLESWMPLIGINIHSSRALYAGIDDDYPFAVLQEVNVVLT
                     DNGGDPHGYPGNGGFNPRQALKPVLHGSNDIGSAGSHNVGYNGLWVWRDSNGNGIFDP
                     PTQGVGGFSFNGDEPLASLDPFYGWEHISEPPGGGAPWWKIKLRFAGWRYTAANLPKV
                     PNNLIPGELQLASEFSYDYFVVARLDSGYKDGSGNATGNPGATFGAEFKAMIEPRRFD
                     QSLNRWEGGIYATSQYYRGRSAIPQLVYVWQNEPDWGNEPWWPERTLNANAAKAHKMG
                     LEVVDLVQTYMGESADPQRFEEIFSFSHFPTLLIHTDQEMANTSMGFGDLGDLFTSRL
                     GKWMDPFGLDAARFRNRHAPDVYSFTPVNVGIRMNLLGQSILMGSSLLGLPQFSFETR
                     PFYFDLYDAPPAGPRSSAYPAPAPAPAIPQIDTWPGSRVFGDFPRLSDWSPEHAKARL
                     LTQKVELRSKHVPMLGFNLAGSSDPVVNQEGNGVSLAELHVAFWGPDFTPSVLRPLDP
                     DKNHNDSLASGVLLWQHALDLTGDTPVDFSSFPAIFVNTEEVSYYGNSPLPLLHKIIP
                     VTGLRWGTAPEFIDLDGDGVPDDMNGDGLVDDRDKAWVLTFRPTNNWQVPQTDIAGTP
                     SGGLDLILSVKTSDTIARFQKFRAVVPSTLPERSADKQKAGIQFWPQVNTGPRAYIKA
                     HGEEGPVQPYYGHDMLEANVPAAVVDMTQRWTDLNIGGSAVPLLGLDLATNTKSTRAT
                     GQNGTGREKSFHVPGASWAADIYAGDVLVDGRYESYEILGNSGDTLYLLSGKPLTGAW
                     RIVQNPTYLEEVMVELYQEGTHNTFNPLTDLLPLDIDQQVSGVALYRDNDNHPANRNG
                     VFDPDIDIPLSLDCPPRFFGQTGDTMKVRFCFSLPGTGDFPKARDRQPRNRQWIYDGF
                     GAAGADYESGPDFFVVVRASNEMHVGDQLRAGIVSWGPATPTEPDPHIWASLPGEDRH
                     DYKKFREFLWAERGLGFVSYFKEPPLYYFLDANRAGQRPDNSGYNWIRSHSAVKARSG
                     VVTARKRPVSPRSLSIDSVSQRVLPVQTLPGQPFDFLIQGKNFGTEPMVGLSGYAVTV
                     LRAANEELHVAIETRDGIIPQSPVTLVIRNPQTGDEMSRSDLFTLGNTVTVNGPKIFG
                     VFPNKVRKEDFPVVLKGANFAGNDFITVHFGETMMPILDVSADGTAMKIGWPQGGLPV
                     PGKLNVSVRAQGANGGQAVLVDGVEYINPEIRNKAGLLGCGPDAARGTSGRNGDLLLL
                     AILGSVLFAMRRRAVRITLPLLLLLLFAAAPGHAFFPKGGWNFFQQLRFATWAMHEFD
                     TDGNGTVDKGEGLEFRIESGPRGFTPAEVEAVRAAFDTWENVPSSYVAFRFAGAIEDP
                     ILPAAVGADYLPMVYMQVTDVSSLDGYGQPDETDFLVPGLDSFQPALTFVLYTIDATV
                     MPVGNNAVMVPAGTILDCDIVVNASLHRPGLLAATTFGVLDLQSTVTHQVGQLLGLSF
                     TPLNNLDPFNSVTLPGAAGGLPVEPAVLQLTGADGLPHAIGATPTMFPIYFLSESSYG
                     DYTGGWRDLAPDDISGISWLYPRKDGQSNFFSIAQEAWTHVRRTTGIPPQPVSGAHVV
                     AWASLSGNDSDQRVPLFSTMTGFYQRHESPQLSGRFALHGLWKQMEVPGRSNEFYEPS
                     YVMTINPLNGIGYDRQAPPGMSASDFDSLQGDFPISYTTSPRPADFFSTNYPSEVYHE
                     DGNIYGMDNNSVGTPLAWSFEKNTVVSKISGKTLPHLLPTNVPMFGDPDTVCPMNIIE
                     NPDGIGDIVTDGGTIPTINSLGGSGGGTGGLRGFNNKLRAFRDDALLRSALGTALVDG
                     FYRVSPFLSRQLQQHGILLSMARSVTGLLAALLETTGWWFLALVAVPGILFTARKRFL
                     KAAAVTAVLLLLLFVPVMAHAGQLPVPTQDLVAKAQVIVTGKIESTQTRFDRNNRIFT
                     DVVLKVQDVVKGNVNRGSNLTFTVIGGIYGSLAMTVPGLPNFTRDEQVLLYLVDYPGY
                     GLMPFGGLRSKVNIFLDETSGEEVLDMSRDDPEVNPPAETKALPATEGEEAEAGEDPL
                     VEESSERMPVADYLDFLRGLVRNQRIR"
     gene            9994..10845
                     /locus_tag="PKN92_09255"
     CDS             9994..10845
                     /locus_tag="PKN92_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49019.1"
                     /translation="MRKQILVPVLLCLLLSGAVQAYGQTVIELAGPQEMCIEWAPPEK
                     ADDLPRIWYDEPGLSRLLVDGVDIWNPETMSEAVTVTFESEGVVYDIYNLKSIGAGLA
                     VYTDWIYNLGTYTLNYTYGDAAPVVRTLVVSESCEPVEGEPVEGEPVEGEPVEGEPVE
                     GEPVEGEPVEGEVVEGEPVEGEPVEGEPVEGEPVEGEVVEGEAVEGETPVEGETPVEG
                     EVVEGETPVEGETPVEGEGEGEDEGCGCAGFEVGLGGVLLGGLTLLALWIGSMLLGGP
                     VWPPVNF"
     gene            11084..>11652
                     /locus_tag="PKN92_09260"
     CDS             11084..>11652
                     /locus_tag="PKN92_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49020.1"
                     /translation="MRQNDFFHGQKAVVLTAVLFFLAPLVYAQTEGESDLELILAGDS
                     TLFYCLQEAPADGLPVIWYEEPGIAALVFEGTDVWSDALASSIVISFAGAEVLCGLDN
                     IAHALATTSWIYTEGSYELLYEFRGGESGTAAAVRNIVVRTCDVGTEGEEVVEGEPVE
                     GEPVEGEPVEGEPVEGEPVEGEPVEGEPV"
BASE COUNT         2510 a         2879 c         3368 g         2895 t
ORIGIN      
        1 catgattaca taaagtgcga caatggcact gaaaaaaaga agcgctgttt catactgtac
       61 actcagtcgt acttcgatga atttcgccgc acccatcagc acaacactgg tgtagagagg
      121 catgaaccca atgatgacca gggaggagaa cagccccaga aaaggggagc cgaagcgctt
      181 agcaaccagt tcgggaaagg tgtgggcgtt cagacggtgc cccatggcgc gggtgcgctt
      241 gcctataaac acaaaggcta tgaagatacc gacagttatg ttcagcacgg taagccagag
      301 gacgctcatg ccgaacatgc ccgcagcacc gccaaagccg acaattgcgg aagtgctgat
      361 aaaggtggca ccgtaggaaa gcgccatgat tgcgggatgg gcgttgcgtc cggcgaccat
      421 gtagtccgtg gcggtgcggg tgttgcggta cccgagatag cccagatatc ctgtaatgaa
      481 aaggtagagc atggtgatga caaacaaaaa cagtgtgctg tgcatacccg gaattcctgt
      541 agtttaaagg agtgtagagt ttgaagaagt aattataatt tctcggataa tttgtccgac
      601 tcttccgccc atttcagatc cgcctcggtc ggttcgctgc cgttgcggtt ccaattgatg
      661 agcccgtagc ccacgcaaag aagggcggca ccgatacata gaatatacgc catgaaaacc
      721 cagaaatctg caagacccag atacatactc agtccttcct ttgcattgat tgcctgaccc
      781 cgcagggcag agggactgct ccagtgtcgg ggccataaaa ctaataatac ctacaaagac
      841 cggcttaaat caagaggtta tttcaagaaa actgttatta ctattcatat aagacatcac
      901 atatatggcg ttgttgttta tgaaccgcag attttacccc tatgtaccga taggtagaat
      961 ataccctgtt ctctgggggc agtgcaagtt tctggcggac atcctgagcc aggaatgagg
     1021 aaagttcacg aagccatcgg tttgataaaa gaccctgttt cagttcaaaa caaaagactg
     1081 tgctgaaaaa aggaatgact tgatattttt caggaaagag cgcaccgggg tcagccttgc
     1141 ccggaattga tattttgtgt tacttttttt ttgaccggtc gtggcttctt tgctgtccga
     1201 aaattatttt acgctttatt attgacattt gaccggaaaa ttgtaataat ctattgacag
     1261 ttgtcaagag tttacttgca agtgaaagat agggaacgcg gaagactggt ctgtccgtgc
     1321 tgttttccgg cagtgccgga cggatgtaaa cagttgacgc ataacaggga ttgtgcgata
     1381 atagcaagca gtgtctctta aaaagtaaat ccgcgttact gcaatccgaa ttcggatggg
     1441 agtgaggata tgattttccg aacagatttg ccaggcaaat ggagccgcat gggtaccgga
     1501 cttacggtag tttttctggt tctgagcgcg atcacaggcg cttttgcgga agcaccggca
     1561 gccgtgaagg aagcactgtc cgccagcgga gtctgggtgg acttgtatca ggaaagcaac
     1621 cctgaacatc aggaatgggc ggcatctgtc tgtgcactgg tacctgctga tgcattactt
     1681 tcggacgcat cggatctgac gatactgctg agtgatgcct ataagccgga agggcttgcc
     1741 ccctgtgcgg atcatcctta cgcctcccag cccgcagcgg ctctttgcag tggttttgct
     1801 gccggggaag atctcatcat tacagcgggc agctgtcttg cagacgggat cgcattggac
     1861 agttatcgct ttatcttcgg gtatgcgctg acagcgcctt cttcagttgt cctggacttc
     1921 agcgcagatc agatttatac gcctgttgag gtggtggctt tttccgatga ggcgggagcc
     1981 gacttcgctc ttgtccgtgt ggatcgcccc attacagttg ccggagcact gccgttgagc
     2041 agcgatgcgc ttgaggcggg gcaggcggta tttactgcgg catacccgtc agggcttcct
     2101 gaaaagctgg ctgcagcttc tgccatgtca gcagaggcag gcgtcggagt tgcactggct
     2161 ccggtcggcg acctttccgg cgcacctgtt atcgtaggtg ccacaggcga ggtggcgggt
     2221 attttatcgg gcggtgctgc agtgtcggcg gatgcgccct gctatgggta tgatcctgcg
     2281 agcggcgccg tgtcagcggt tggttttacc gcttcatccg ctttttcctc tttcgtgagc
     2341 ggtgtggctg cggagaagca ggcagctgaa ggagagacac cggatgttcc ggcagcagac
     2401 tttactgtcg attctctgat ccgggacggc gaggcgctgc ttcctcttca cgactgggtg
     2461 cctctcttcc gtttttccat gagttatggc gaagaacctg ctccgcggga acttgaagaa
     2521 ctcaagtacc gtctcatcaa cgatgatcct gaagagcgcg agtatttggt gcgtccggat
     2581 ctgaggcaga gcgatattct tgagttcggt cttttctccg acagtttcag tacggacaca
     2641 gaagggaatg aagacaggta cggcgctttg agcaccgatg atccgcttct ctttacctgg
     2701 gatgcgacgg gtgcccccag cggctctgcc cggggtctgg agtattccat gaatttcaga
     2761 ggacgcaatg ttgttgcggg accgcagatt gatgatgtga tgcccgacgg cagagtatat
     2821 attgtcgctg tacgtacttc gcccacctgg cgaagcgcac tgagcatggg tgtggaagtg
     2881 actatggcgc gtatggtcga gccaaggaca ggggtattta ttccggagct cgacagttac
     2941 agtccgaact tttatgaagg ggaaaaactg gaagacagcg gcttttactc ctcgtccttc
     3001 ggcgtctatg atcctaccgg gccggatctg ggtggaatga gcggcttcga aagctggaag
     3061 gatattgcga cgcctaattt tttccagtac accccttatc tgtccgtccc tctgtctgag
     3121 ttttcccgtc cccgctggaa caagccggat tcgctgatca gtatcgcaac aggtcagttt
     3181 atgaatatcc gcacgcttat gccgctggaa tcctggatgc ctcttatcgg catcaatatt
     3241 cattcgagcc gggcactgta tgcgggtatc gatgatgatt atccttttgc ggttctgcag
     3301 gaagtgaatg tggttctgac cgacaatggc ggtgaccccc acgggtaccc gggcaacggc
     3361 ggcttcaatc cccggcaggc attgaaacct gtgctgcacg gctccaatga tataggcagc
     3421 gcaggcagcc ataatgtggg ttacaacggt ctgtgggtct ggagggacag caacggcaac
     3481 ggtatttttg atccgccgac acagggcgta ggtggtttct cttttaacgg cgatgaaccg
     3541 cttgcctctc tggatccttt ttatggctgg gagcatatca gtgagcctcc cggcggcggc
     3601 gccccgtggt ggaagatcaa acttcgcttt gcgggatgga gatacacagc ggccaatctg
     3661 cctaaagttc ccaacaacct gatcccgggc gaactgcagc ttgccagcga attctcttat
     3721 gattattttg ttgttgcccg gctggacagc ggctacaaag acggttccgg caatgcaaca
     3781 gggaaccctg gtgcgacttt cggagccgag ttcaaagcca tgatagagcc ccgtcgcttt
     3841 gaccagagtc tgaaccggtg ggaaggcggt atttatgcga cttcccagta ttaccgcggc
     3901 agatctgcga taccgcagct cgtgtatgtg tggcagaatg agccggactg gggcaatgag
     3961 ccctggtggc cggagcggac cctgaatgcc aatgcagcca aggcgcacaa gatgggtctg
     4021 gaggttgtgg atctggtgca aacctatatg ggtgagagtg ccgatcctca gcgtttcgag
     4081 gagatttttt ctttctctca cttcccgaca ctgctgatcc atacggatca ggagatggcg
     4141 aatacgtcca tgggtttcgg tgatctgggc gatctcttca cctcgcgtct gggcaaatgg
     4201 atggatccct tcggtcttga tgcagccaga ttccgcaacc gtcatgcacc ggacgtttac
     4261 tctttcacgc ctgtcaacgt cggcatccgt atgaatttgc tgggacagag catccttatg
     4321 ggatcttctc tgctgggttt accccaattc tcttttgaga cgcggccctt ttattttgat
     4381 ttatacgatg ccccgcctgc gggtccccgc tccagtgcat accctgctcc tgccccggca
     4441 ccggctattc cgcagattga cacctggccg ggcagtcgtg tgttcggcga tttcccccgg
     4501 ttgagtgact ggagtccgga acatgcgaag gcgcggttgc tgacgcagaa ggtagaactg
     4561 cgaagcaagc atgtgccgat gctcggcttc aatcttgccg gaagctctga tcctgttgtg
     4621 aatcaggaag gcaatggcgt ttcactggcg gagctgcatg tggcgttctg gggtcccgac
     4681 ttcactcctt ccgttctgcg tcctctggat cccgataaga atcacaatga cagccttgcc
     4741 tccggagtgc tgctgtggca gcatgcactg gatcttacag gcgatacgcc cgttgatttt
     4801 tctagtttcc cggcgatttt tgtgaatacg gaagaagtga gctactacgg aaattctccg
     4861 ctgccgctgc tccataaaat cattccagtt acggggctgc gttggggcac tgctccggaa
     4921 tttatcgatc ttgacggaga cggtgttcct gatgacatga acggtgatgg cctggtggat
     4981 gaccgcgaca aggcgtgggt tcttactttc cgtccgacca ataactggca ggtgccgcaa
     5041 accgatatcg ccggaacgcc ttcgggcgga ctggatctta ttctgtccgt aaagacctcg
     5101 gacacaatcg cccgtttcca gaaattccgt gctgtggtgc cttccacgct gccggaaaga
     5161 tctgcggata agcaaaaagc gggtatccag ttctggccgc aggtgaatac aggcccgaga
     5221 gcctatatca aggcgcatgg tgaagaagga ccggtgcagc cctactacgg gcatgacatg
     5281 ctggaagcca atgtcccggc tgctgttgtg gatatgacac agagatggac agatcttaat
     5341 attggcggca gtgctgttcc cctgctcggg cttgatctgg cgaccaatac gaaaagcaca
     5401 cgcgcaaccg ggcagaatgg taccgggcgc gagaaatctt tccatgtgcc cggtgcatcc
     5461 tgggcagccg atatttacgc cggtgatgtg ctggtcgatg ggcgctatga aagttatgag
     5521 attctcggca acagcggcga taccctgtat cttctgagcg gcaagccgct tacgggcgca
     5581 tggcgcattg tgcagaatcc cacctatctt gaagaagtga tggtggagct gtatcaggaa
     5641 ggaacacaca acaccttcaa cccgctgacc gatctccttc ctcttgatat agatcagcag
     5701 gtgagcggcg tggcgctgta tcgtgacaat gacaaccatc ccgcaaaccg gaacggtgtt
     5761 tttgatccgg atattgatat cccgctgtcg ctggattgtc ccccccgttt cttcggtcag
     5821 accggcgata cgatgaaagt gcgcttctgc ttctctcttc ccggcacagg cgactttccg
     5881 aaagcacggg atcgccagcc cagaaacagg cagtggattt acgacggctt cggtgctgcc
     5941 ggcgccgatt atgaaagcgg accggacttc tttgtcgttg tccgcgcttc caatgagatg
     6001 catgtgggcg atcaactccg tgcaggtatt gtgtcctggg gtcccgctac acccacagag
     6061 cccgacccgc atatctgggc gagcctgccg ggagaagaca ggcacgacta taaaaaattc
     6121 agggaatttc tctgggcgga gcgtggcctt ggctttgtga gctatttcaa agaaccgccg
     6181 ctttactact tcctggatgc gaaccgtgcc ggtcagcgtc cggataacag cgggtacaac
     6241 tggattcgca gtcacagtgc cgtgaaggca cggtccggcg ttgttaccgc ccgcaaacgt
     6301 ccggtcagcc cgcgctcgct cagcatagat tcggtttccc agcgggtgct gccggtgcag
     6361 actcttcccg gacagccttt tgatttcctg attcagggca aaaatttcgg tacggaaccg
     6421 atggtgggcc tgagtggtta tgcagtgact gttctgcgcg ccgccaatga ggagctccac
     6481 gtagccatcg aaacgcgcga cggcattatt ccgcagtcac cggttaccct tgtgatccgc
     6541 aacccgcaga caggcgatga aatgagtcgt tccgacctgt tcacgctggg caacacggtc
     6601 actgtgaatg gtccgaaaat tttcggagtg tttccgaata aagtgcggaa agaagatttc
     6661 ccggtggtgc tgaaaggtgc aaactttgcc ggaaatgatt tcataacagt tcatttcgga
     6721 gagacaatga tgccgattct ggatgtttcc gccgacggca ctgcgatgaa gatcggatgg
     6781 cctcagggcg gcttgccggt acccggcaaa ctgaatgtat ctgtgcgtgc ccagggcgcc
     6841 aatggcggtc aggcggttct ggtcgatggt gtcgagtaca ttaatccgga aatccggaac
     6901 aaagccggat tgctcggctg cggtcctgat gctgcccggg gaacctccgg cagaaatggc
     6961 gatctgctgc ttctggcgat tctgggttca gtgctttttg cgatgcgtcg ccgggccgtg
     7021 cgaattacgc tgcctctgct cctgctgctt ctttttgcag cggcaccggg tcacgctttt
     7081 ttccctaaag gcggatggaa tttcttccag cagctgcgct ttgccacctg ggcaatgcat
     7141 gaatttgaca cggacgggaa cggcaccgtt gataaaggag aagggctgga attccgtatt
     7201 gaaagcggtc cccggggctt tactccggcg gaggtcgagg ctgtccgcgc cgcttttgac
     7261 acctgggaaa acgtgccgtc cagctatgtg gcgtttcgct ttgccggtgc gatcgaagac
     7321 ccgattttac cggctgctgt gggcgcagac tacctgccca tggtgtacat gcaggtaaca
     7381 gatgtttctt ctttggacgg ttacggacaa cctgacgaaa cagactttct ggtgcccggt
     7441 cttgacagct tccagccggc gctgactttt gtgctgtata cgattgatgc aacggtcatg
     7501 cctgtgggca ataatgcagt tatggttcca gcaggcacca ttcttgattg tgatattgtc
     7561 gtgaatgcga gtctgcaccg tccaggcctg cttgcagcaa ctaccttcgg cgtgcttgat
     7621 ctgcagtcaa cagtgacaca ccaggtcggt cagctgctgg gtctttcgtt cacgcccctg
     7681 aacaatcttg atcctttcaa ttctgtaaca cttccgggtg cggccggcgg gctgcccgtt
     7741 gagcctgcag ttctgcagct gaccggggcg gacggactgc cccatgccat cggtgctact
     7801 ccgaccatgt tccccatcta tttcctgagt gaatcctctt atggtgacta tacgggcggc
     7861 tggcgtgatc ttgcgcctga tgatatttcc ggtatttcct ggctgtatcc gcgcaaggac
     7921 gggcagagta atttcttcag tattgcacag gaggcctgga ctcatgtccg ccgcacaacg
     7981 ggtattccgc ctcagccggt ctccggtgcg catgtggttg cctgggcaag tctttccggc
     8041 aacgactcag atcagcgcgt tcctcttttc agcaccatga ccggtttcta tcagagacat
     8101 gaaagcccgc agctgagcgg tcgctttgca ttgcacgggc tttggaaaca gatggaagta
     8161 ccgggaagaa gcaacgagtt ttatgagcct tcttatgtga tgaccatcaa tccgctgaat
     8221 ggtataggct atgaccgtca ggcgcccccg ggcatgagtg cttctgattt tgacagcctg
     8281 cagggtgact tcccgatcag ctatacgacg agtccccggc ccgctgactt cttcagcacc
     8341 aattatcctt ccgaggtcta tcacgaagac ggtaatattt acggcatgga caacaattcg
     8401 gtgggcacgc cgctcgcctg gagctttgag aagaatactg tggtcagcaa aatctcagga
     8461 aaaacgcttc ctcatctgtt accgaccaat gtgcccatgt ttggtgatcc cgatacagta
     8521 tgccccatga acatcatcga aaatcccgat ggtatcggtg atatcgtcac cgatggcggc
     8581 acgattccga cgataaacag cctgggcggt tccggtggcg gtacaggcgg actgagagga
     8641 tttaataaca aactgcgcgc tttcagagac gacgccctgc ttcggtctgc actgggcacg
     8701 gccctggtag atggatttta tcgcgtgtcg cccttcctct ccagacagct ccagcagcat
     8761 ggtatcctgt tgagtatggc gcgcagcgtg acggggcttc ttgcggctct gctggaaaca
     8821 accggctggt ggttccttgc acttgttgcg gtgcctggca ttttgttcac ggcgcggaag
     8881 cgttttctta aagcggcggc tgttacggca gtgcttctgc ttctgttgtt tgtgccggtc
     8941 atggcgcatg ccggacaact gcctgtcccg actcaggatc ttgtagccaa agcgcaggtc
     9001 attgtgacag ggaagatcga gtcgacgcag acccgttttg accggaacaa ccgcatcttt
     9061 accgatgtgg tattgaaagt gcaggatgtg gtcaaaggaa atgttaaccg gggatcgaat
     9121 cttaccttca ctgttatcgg cggtatttac ggatccctcg ccatgacggt gcccgggctt
     9181 cccaatttta cgcgggatga acaggtgctg ctgtatcttg tagattatcc gggatatggc
     9241 ctcatgccct tcggtggtct gcgcagtaaa gtgaatatct ttctggatga gaccagtgga
     9301 gaggaagtgt tggatatgtc gcgcgatgat cctgaagtga atccgccagc cgagaccaaa
     9361 gcacttccag cgacagaggg agaagaggcg gaagccggcg aggacccact tgttgaagag
     9421 tcctccgagc ggatgcctgt ggcggactat ttggattttc tccgcggtct cgtacggaat
     9481 cagcgcatcc gctgactctt cgcatttctg atgtgtaaac aagcccgcat ggtggaaaaa
     9541 gccatgcggg tttttctttt acaaatatga aacttctgga gcagttcgat tgtagggtaa
     9601 ttgaaaggtt gacagcgaca gtttcaggct tcgcctcaat ccgggaaagt ttcgtgactg
     9661 gttcctctaa cctgctggtc aaataaaaaa tcgctggctc agaatacggg agagcgtccc
     9721 gggtagattc cggatgcgtc gaatcatccc agctgcgggc tcctgaatct ttgtcttttc
     9781 gctcttattt cgtccattat tttgcccaat tgttgacaaa taaaaggaga tatgctacac
     9841 tgtgagttga gtgagtttgg agacaggtcc gtggctgcag gtaaatgttt tgaactggca
     9901 gtcagagtct gtctttgtaa ggtgaatgtt ggtcaatttg ttttgtttgc ttttaagcgg
     9961 tttatttcat cacaacaaag gagaatgtgt cctatgcgta aacaaattct ggttcccgtt
    10021 ctgctgtgcc tgttgctttc aggggctgta caggcgtacg gccaaaccgt tattgaactt
    10081 gctggccctc aggaaatgtg cattgaatgg gcgcccccag aaaaagctga tgatttgccg
    10141 cgaatttggt atgacgagcc cggtctctcg cgccttcttg tggacggcgt agatatctgg
    10201 aaccccgaaa caatgtcgga agccgtcaca gtgacctttg agtcggaagg tgttgtttac
    10261 gatatctaca acctcaaatc tatcggtgcc ggtctggctg tctatacaga ctggatttac
    10321 aatcttggaa cctatacact taattatact tatggcgatg ctgcgcctgt agttcgtact
    10381 ttggtcgtca gcgaaagttg tgagccagta gaaggtgagc ccgtggaagg tgagcccgtg
    10441 gaaggtgagc cagtagaagg tgagcccgtg gaaggtgaac ctgttgaagg tgaacccgtt
    10501 gaaggcgaag ttgtagaagg tgaacccgtt gaaggcgaac ctgtagaagg cgaacctgta
    10561 gaaggcgaac ctgtagaagg cgaagttgta gaaggtgaag ctgtagaagg cgagacgcct
    10621 gtggaaggtg agacgcctgt ggaaggtgaa gtcgtagaag gcgagacgcc tgtggaaggt
    10681 gagacgcctg ttgagggcga gggtgaaggt gaagacgaag gctgcggctg cgccggcttt
    10741 gaagtcggtt tgggcggtgt tctgctcggc ggtttgacgc tgcttgccct ctggatcggc
    10801 agtatgctgc tcggtggacc ggtttggccg cctgttaact tctaagcctg tttcctgtta
    10861 atattttgcg gccagccaca caagtggctg gtcgctttgt ttttgtgtat tcgtatactt
    10921 atcgccagta taaatgtggc gtcctctcat ggagttgaga gcataaaggg tcgaactctt
    10981 catttacagt gtgtgcgtgt ttgcacgaaa aaaaacatat ccgggaatgt agcgggtatt
    11041 caacctctgt tcagttcgct gactaaaacg gaaaaggatt tttatgcgcc agaacgattt
    11101 ctttcatgga caaaaggctg ttgtgctgac ggctgtgctg ttcttcctcg cccctttagt
    11161 gtatgctcaa acggaaggag agagcgatct tgaactgatc ttagcaggcg acagcacgct
    11221 tttttactgc ttgcaggagg cgccggcaga tggcttgccg gttatttggt atgaggagcc
    11281 cgggattgct gccctggtct tcgaaggtac tgacgtctgg tctgatgcgc tggcgtcttc
    11341 tattgtcatc tcctttgccg gcgcagaggt tctgtgcggc ctggacaata ttgctcatgc
    11401 tcttgcaact acttcctgga tttatacaga aggcagttac gaacttcttt acgaatttag
    11461 aggcggcgaa tcaggtactg ctgctgcagt gagaaacatc gttgttcgta cttgtgatgt
    11521 aggtaccgag ggagaagaag tggtagaagg ggagcccgtg gaaggggaac ccgtggaagg
    11581 tgagcctgtg gaaggggagc ctgtggaagg ggagcctgtg gaaggtgagc ctgtagaagg
    11641 tgaacctgtg ga
//
LOCUS       DAOPWB010000097        11465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5758_length_11465_cov_5.386503, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000097 DAOPWB010000000
VERSION     DAOPWB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5758_length_11465_cov_5.386503"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..811)
                     /locus_tag="PKN92_09265"
     CDS             complement(134..811)
                     /locus_tag="PKN92_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ49021.1"
                     /translation="MRILLVEDSKRLQTYIARGLRHQGFAVDQAFDGEEGLFLAESGD
                     YDVVILDLMLPELDGFSLLQRLRERDVQTHVLILTARDAVADRIKGFDIGADDYLIKP
                     FAFEELVARIQALLRRRYGVKTSVLRFGDLEINLSRREALKGRRHLPLRPREYALLEL
                     LALRQGAVVSRAEIERQIYDERVSPMSNVVDSAVCNLRKMIDTPGAPSWIETRRGMGY
                     LFGEPQA"
     gene            complement(930..2225)
                     /locus_tag="PKN92_09270"
     CDS             complement(930..2225)
                     /locus_tag="PKN92_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ49022.1"
                     /translation="MSTKDKGTDLSRRGFMKTTGALGMGVYLGSSLGSTKTVEAQTAR
                     IREPRKGPLHTYLTFSDDPTTSMDINLAIQRKGEEVMVYYDTAPHGDDINAYAMKCKA
                     QHYSTLVELYDRRALYRADLRGLKPGTTYYFVAGDEKYGYGDMRSFRTLPGGNAPVRF
                     INGGDMNIGPRNRKLLKWAGEQDADFLVIGGDLPYANGIYAEFTDWIQWFHNQDDLLH
                     VGGRMVPLVTAIGNHETNGYETEDIYLRSPFYMSFFGRQGQGGVYHSHKMGDNIALIM
                     LDSGHLVPHGGTQAEWLEQELKKFQHVKYKFAAYHVPLYPAYRDFDGAPSAAGREHWL
                     PLFDKYGLTVGLEHHDHVFKRTKPLKGNQVVSAGEGTVYLGDGTFGVDPRNMDDKPLH
                     WYNEKQGGIAHFWVIDAKPERLEIKAIDEDGKVIDELTI"
     gene            2596..3972
                     /locus_tag="PKN92_09275"
     CDS             2596..3972
                     /locus_tag="PKN92_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ49023.1"
                     /translation="MSTNSSKKTALSSALSRRAFLQCSTMLTGAFVLGAPAIVRGQNL
                     NDKLNIAIIGVGGRGTGNLGGVQGGNNITVLCDVNQNHLDKFSKHFSAARLEKDFRKV
                     YDHPEEFDAVVVSTCEHTHAFATLPALQLGKHVYCEKPLTHGIWEARVIQEAAAKAGV
                     ATQMGTQIHAENNYRRVVELIQTGAIGSVKEVHVWVSRAWGWHATKEEAEQAKDIVFI
                     QDFPADTSPIPEGLDWDLWLGPAPERPFNDVYFPGPKWYRWWDFGSGTMSDLGSHYID
                     LPFWALELDHPRTIEASGPPPHPHLAPASMQVTYEYAARGERGPLSLTWYQGVNKPQI
                     WTDGGIPKWDDGILFIGDKGMLLSNYGKYELLPKKEFKGFKPPEPFIPESKGHYAEWI
                     NACKTGEPTTCNFAYSGKLTEANHLGNIAYRLGKKLEWDPTSLSCPNAPEAAPLIRRA
                     YRAPWELA"
     gene            complement(4065..5735)
                     /gene="ilvD"
                     /locus_tag="PKN92_09280"
     CDS             complement(4065..5735)
                     /gene="ilvD"
                     /locus_tag="PKN92_09280"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNZ49024.1"
                     /translation="MGSSTVKDGFERAPHRSLLYATGQVKCSKDFKKPFIGICNSFVE
                     IVPGHAHLAEFGRIAKAAIEEAGGIAFEFNTIGVCDGIAMGHAGMRYSLPSRELIADS
                     VETMVRAHDFDALLCVPNCDKITPGMLMGAMRCNIPTVFVSGGPMRAGKMDDGTAVDL
                     ITVFEGVGKYKIGEMSEAELEDLEQKACPGCGSCSGMFTANSMNCLCEALGVALPGNG
                     TILADTEERRDLVRRAARQLLKVLEADLRPRDIVNAESIDNAFALDMAMGGSTNTVLH
                     TLAIAQEAGVDYPLERLNEVSARVPNICKVSPSYKWHIEDVGRAGGISAILGELMERP
                     GILFPDCMTVTLRSLGENVAGARTLDTEVIKTVDAPYSPTGGLAVLFGNLAPQGAVVK
                     EAGVDPAILRHTGPAVVFESEEDAMHAILEGKVVAGEIIVIRYEGPQGGPGMREMLAP
                     TSAVMGAGLGASVSLITDGRFSGGTRGACIGHVSPEASQGGPIGLVQTGDIIEIDIPG
                     RTMNVKLSAEALAAREKNRPAPPDRALSGWLKRYQRLVTSANTGAVLK"
     gene            6347..6481
                     /locus_tag="PKN92_09285"
     CDS             6347..6481
                     /locus_tag="PKN92_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49025.1"
                     /translation="MDTSFVQESNSGTMAAILNQNFQKKFLYKDWLCYYRVSGITPCR
                     "
     gene            6594..8063
                     /locus_tag="PKN92_09290"
     CDS             6594..8063
                     /locus_tag="PKN92_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNZ49026.1"
                     /translation="MSYAISEYHDTDAIYRDLDTLMALPPVTLRRDAMQQWLEQLNQR
                     CAKSKAMIAEAKEFIPGGVQHNLSFNYPFPLVMDRAEGPYLYDIDGNRYIDFLQAGGP
                     TVLGSNPPAVLEQVIELLRHCGPSTGLFHEFELKIAKEIAKHMPAVEMFRMLGSGTES
                     VMAAIRVARLATGKKNIIKMGGAYHGWSDQMVYSLKIPKTRRFEAHGIPRACVKYTTE
                     AANNNLKDLERRLFKNRFRGGTAAVIVEPVGPESGTRPVARDYNAGVRELCDRYGALL
                     IFDEVVTGFRLGMGGAQGYFNVRPDLTIFGKVVAGGYPAAGGVGGRRDLIQRMAAGLE
                     SGKKRAYVGGTLAANPLSAAAGYFAIQEMERTNACVKAGQAGDRLTRGLRTMIEEMKL
                     PFVTYNQGSICHLETIGGMFIKLDYLRILKVLKEIKVRKHAMEEYGAAYMAEGIITLA
                     GSRLYTSMADTDEVIDDALARFRRVFEKADWDGAKGKQS"
     gene            8060..8722
                     /locus_tag="PKN92_09295"
     CDS             8060..8722
                     /locus_tag="PKN92_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNZ49027.1"
                     /translation="MSDIHAARKAVRAMGLRLLETGLVIGTWGNVSVRAGGKVVITPS
                     GADYTLLQEEDMPVVDLETGAYEGAKPSSEKGLHLEVYRQRKEINAVIHVHSPHAATL
                     AAARKPLPPILDDIAQLIGPGVRVADYALPSTKKIVRKTLRALKGRMAALMANHGAIC
                     LGRDLEEAYLCCQVLEKGCKAYIEAEFLGGAKGINYFEAALMHQFFLRKYSRQWKDKN
                     PT"
     gene            8755..10347
                     /locus_tag="PKN92_09300"
     CDS             8755..10347
                     /locus_tag="PKN92_09300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNZ49028.1"
                     /translation="MKADKTYVLAVDLGTSGCKTALVSLGGEVAAWHFEPVETRLFGN
                     GGAEQEPQDWWDAFLKTARAVLDSAGAAPESIAAVCCSTQGEGTIPVDAEGKVLHPCI
                     LWMDTRGAEYLREITGKTLRVAGYGPLRLRRWIRLTGGAPSLTGKDPAAHMLLIKHAM
                     PDIYRNTAKFLNVPDYMNLRLTGRIVATPDSILTSWVTDNRDPARIVYHDGLVRNSQI
                     EKDKFPEIVPSTEVIGTLLPEVAEQLGLPSTVPVVAGSIDTTAAAIGSRAVADYDAHL
                     YLGTSSWLAAHVPFKKTDIFASLASVPCALKDRYLMIALQATAGGNLTFLRDKILYHK
                     DELLAEASVPDVYALMDQIVERTPPGSNGVLFTPWSFGERSPVEDPLIRAGIHNLSLE
                     NSREDIIRAFFEGIAFNNRWLLKPVERFLGRKLEAITLTGGGANSDVWCQIFADVLQR
                     PIRQLADPIQVNVLGAAFIAFLGLGRITKEEIPRIVRHKAVYEPRAELQTLYNDLFRE
                     FVALYERNKAIHHRLNRKRTRS"
     gene            10837..>11465
                     /locus_tag="PKN92_09305"
     CDS             10837..>11465
                     /locus_tag="PKN92_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNZ49029.1"
                     /translation="MCVIWTCALTLVAMVPVARAGESDDAGSLALRPAKVERILLGGV
                     VGDRVQANIDAWLLSAPLANPGMIEMFRVRDRKPVPELVPWAGEFIGKYLISAIQARR
                     MTDDDRLDSFLREVIPEFISTQAEDGYLGPFRKEERLLGHWDLWGHYHAILALLMWYE
                     DTGDPAALACARRTGDLICSLYLNTNRRPKHAGADEMNLAIIHGLGRLYR"
BASE COUNT         2674 a         3395 c         3012 g         2384 t
ORIGIN      
        1 cgctccagca aaacagagtc aaaatgcaag cccagctcat actgcacata ggcaaaaaga
       61 accagcccgg atatcaggaa catgacaacc agagaaaggc tgatccagag cagtagccgg
      121 tgtcgcagag agttcacgcc tggggttctc cgaaaaggta acccatgccg cgccgggttt
      181 ctatccagga gggagcgccc ggagtatcaa tcatcttccg aagattgcat acggcagagt
      241 ccaccacatt actcatggga ctgacccgct catcataaat ctgacgttca atttccgcac
      301 ggctgaccac tgcgccctga cggagcgcaa gtaattcaag gagtgcatat tcgcgggggc
      361 gaagcggcag atgccgcctc cctttcagcg cctcccgccg ggaaagattg atttccagat
      421 caccgaaacg aagaacggag gtcttcacgc cgtaacgccg ccgaagcaat gcctgaatcc
      481 gggcgacaag ctcctcgaag gcaaaaggtt tgatcagata atcgtcggca ccgatatcaa
      541 agcctttgat ccgatccgct acagcgtccc gcgctgtgag aatgagaaca tgtgtctgca
      601 catccctttc ccgaaggcgc tgaagcagag aaaacccgtc cagctccggc agcataagat
      661 ccagaatcac aacatcgtaa tcgccggact cagccagaaa aagcccttct tctccgtcaa
      721 atgcctgatc aaccgcaaag ccctgatgac gcagaccccg ggcaatgtac gtctgcaggc
      781 gtttggaatc ttcaaccaga agaatacgca tgaccttcga tttccgtcag aatgaacaaa
      841 ccttttgtaa gtccagatga tacgcaggta tttgaaaaag tgccgacccg aaaacatttc
      901 cgggccggca cgaaaaaaga ggagggtaat taaatggtca attcgtcaat cactttgccg
      961 tcttcgtcga tggctttgat ttcgagccgt tcaggcttgg catctatcac ccagaagtgg
     1021 gcaatgcccc cctgcttttc attgtaccag tgcagcggct tgtcgtccat attccgggga
     1081 tccacaccga aggtaccatc accaagatag accgtgccct ctccggcaga cacaacctga
     1141 ttgcctttca ggggcttcgt ccgcttgaag acatggtcgt gatgttcaag accaacagtc
     1201 agcccgtatt tatcaaatag aggcagccag tgctcgcgtc cggcagcgga gggcgcaccg
     1261 tcaaagtcac gataggcggg gtacagcggc acatggtatg ccgcaaattt gtacttgaca
     1321 tgctggaatt tcttcagttc ctgctccagc cattccgcct gcgtgcctcc atgaggaacc
     1381 agatggccgc tgtccagcat aatcagcgca atattgtcgc ccatcttgtg ggaatgataa
     1441 acaccgccct gaccctgacg accgaaaaaa ctcatgtaga agggcgaacg cagataaatg
     1501 tcctccgttt catacccgtt ggtttcatga ttgccgatcg ctgtcaccag aggaaccatt
     1561 ctcccgccca catggagcag gtcatcctga ttatggaacc actgaatcca gtctgtgaat
     1621 tcagcataga tcccgttggc atagggaagg tcaccgccga ttaccagaaa atccgcatcc
     1681 tgctcacccg cccatttcag cagcttgcgg ttgcgcggac cgatattcat atcgccgccg
     1741 ttaataaaac gtaccggcgc attaccgccg ggaagggtgc ggaaggagcg catgtctccg
     1801 tagccgtatt tctcgtcgcc cgccacaaaa tagtaggtgg tgcccggctt cagaccgcgg
     1861 aggtcggcac ggtaaagagc acgacggtca taaagctcga caagcgtgct gtaatgctgc
     1921 gctttgcact tcatggcata ggcgttgatg tcatcgccat gcggcgcggt gtcataatac
     1981 accattactt cttcgccctt gcgctgaatc gccagattga tgtccatact ggtcgtggga
     2041 tcatcggaaa aagtaaggta ggtatgcagc ggtcctttac ggggttcccg tatgcgtgcc
     2101 gtctgtgctt ccaccgtttt tgtgctgccc aggctgctgc ccagatagac gcccatacca
     2161 agagcgccgg ttgttttcat gaatccgcgc cgggagagat cggttccctt atctttcgtg
     2221 gacataaaaa cgactccttt cttaatgtgt tctatgcgct aaaagcgctg ctgcagaggc
     2281 atcacaaacc tccgtttctc cgggaaattc ccgcttcagt tctttccaga aaaaaataca
     2341 agaatgacac ctcgggaaga aatgccggac gcccagcaac gcaggatacc tgtacgcttc
     2401 aaaacatcct gaatacggct gcttttttcg cctcttgtaa aatcctgttt cctatggtac
     2461 tacccgactg tgcggcaagt caaaggaaga tcagaaaagg aaagcaaatt ttggaaataa
     2521 agatgcgctt tgctatgctg atatacggat tcgtgcttgg caaaacccat cattacagag
     2581 aaaggatatg atgatatgtc taccaactct tcgaagaaga ccgcattgag cagtgccctt
     2641 tcccgccgcg ctttcctgca atgctccacc atgctcacag gcgcatttgt gctcggtgct
     2701 ccggctatcg tgcggggaca gaatttgaac gacaaactga acatcgccat tatcggcgtg
     2761 ggtggccggg gcacaggaaa tctcggtggc gttcagggcg gcaacaacat tactgtcctt
     2821 tgcgacgtaa accagaatca tcttgataaa ttttccaaac acttttccgc cgcccgtctg
     2881 gaaaaagact tccgaaaagt gtatgaccat cccgaggaat tcgacgccgt cgtcgtaagc
     2941 acctgcgagc atactcatgc cttcgctacc ctgccggcgc tgcagctggg caaacatgtg
     3001 tactgcgaga aaccgctgac ccacggcatc tgggaagccc gcgtaattca ggaagcagcg
     3061 gcaaaagccg gtgtcgccac gcagatgggc acccagatcc atgcggaaaa caactatcgc
     3121 cgtgtcgtcg aactgatcca gaccggtgcc atcggttccg tgaaggaagt ccatgtgtgg
     3181 gtttcccgcg cctggggctg gcacgccacg aaagaagagg cggagcaggc aaaggacatt
     3241 gtcttcatac aggattttcc tgctgacaca tcgcctattc ctgaagggct cgactgggat
     3301 ctgtggctgg gtccggcgcc cgaacgcccc ttcaacgatg tctatttccc gggaccgaaa
     3361 tggtaccgct ggtgggattt cggaagcgga accatgtccg atctgggcag ccattacatt
     3421 gatctcccct tctgggcact ggaactcgat catccccgga ccattgaagc ctccggtccg
     3481 ccgccccatc cccatctggc acccgcttcc atgcaggtga cctatgaata cgcggcacga
     3541 ggcgagcgcg ggcctctctc tctcacctgg tatcagggtg tgaataaacc gcagatatgg
     3601 acagatggcg gcatcccgaa atgggatgac ggcattcttt tcatcggcga caaaggtatg
     3661 ctcctttcca attacggaaa atatgagctg ctgccgaaaa aagaattcaa aggcttcaag
     3721 ccgcccgagc ctttcatccc cgaatccaaa ggacattatg cggaatggat taacgcctgc
     3781 aagacgggcg agcccaccac ctgcaacttc gcctattcag gaaaactgac agaagcgaac
     3841 catctgggca atatcgccta tcgcctgggc aaaaaactgg aatgggatcc cacatccctc
     3901 tcctgcccca atgcacccga agcagcgccg ctgatccgcc gcgcctatcg cgccccctgg
     3961 gaactcgcct gaacaatttc cgggcactga actgaaataa tacgcccggg cactgtcgat
     4021 agaagacatt gcccgggctt ctttttaccg gaaaaccgga atgcttattt cagcaccgcc
     4081 cctgtattgg cactggtcac caggcgctga tagcgcttga gccagccgct aagcgcacga
     4141 tccggcggcg ccggacggtt cttttcacgt gccgcaagcg cttctgccga cagcttcaca
     4201 ttcatggtgc gaccgggaat atcgatttcg attatgtcgc cggtctgaac cagaccgatg
     4261 ggtccgccct ggctcgcttc cgggctcaca tgaccgatgc aggctccgcg tgtaccgccg
     4321 gagaaacgcc cgtccgtgat caggctcaca ctcgcaccca gaccggcgcc catgaccgcc
     4381 gaggtcggcg ccagcatttc acgcatgccc ggtccgccct gcgggccttc gtaccggatt
     4441 acaataatct caccggcgac caccttgccc tcgagaatgg catgcatggc atcttcttcg
     4501 ctctcaaaaa ccacagccgg acccgtatgc ctcaggatgg cgggatccac acccgcttcc
     4561 ttcaccacgg ctccctgtgg cgcaagattc ccgaaaagca ccgccagccc gcctgtggga
     4621 ctgtagggcg catccacagt cttaatcact tccgtatcca gcgtccgggc accggcaacg
     4681 ttttcgccga ggcttctgag cgtcaccgtc atgcagtcgg ggaaaagaat gccgggccgc
     4741 tccatcagct cgccgaggat ggcactgatg ccgcctgctc tgcccacatc ttcaatgtgc
     4801 catttatagc tgggtgaaac tttgcagata ttgggaaccc gggcactgac ttcgttcaga
     4861 cgctccagcg gataatccac acccgcttcc tgggctatcg ccagagtatg cagcaccgta
     4921 ttggtggagc cgcccattgc catatcaagt gcgaaggcgt tatcgatgga ttcggcattc
     4981 acaatatcgc ggggacggag atccgcctcc agcactttca gcagctgccg ggcggcacgt
     5041 ctcaccagat cacgacgctc ttcagtgtcg gcaaggatgg taccattgcc gggcagcgca
     5101 acacccagtg cttcacacag gcagttcatg gaattggcgg taaacatgcc cgagcaggag
     5161 ccgcagccgg gacacgcttt ctgctccaga tcttccagct cggcttcact catctcgccg
     5221 attttgtatt tacccacgcc ttcgaaaacg gtgatcagat ccaccgccgt gccgtcatcc
     5281 attttcccgg cacgcatagg accgcccgac acgaatactg tcgggatatt gcagcgcatg
     5341 gcacccatga gcatgcccgg cgtaatttta tcgcaattcg ggacacagag cagagcatca
     5401 aaatcatggg cgcgcaccat cgtttccacg ctgtcggcga tgagctcacg ggaaggaagg
     5461 ctgtaccgca ttccggcgtg tcccatggca ataccgtcgc agacaccgat ggtgttgaac
     5521 tcaaaagcaa tgccgcctgc ttcttcgata gcggctttgg caatccgccc gaattccgca
     5581 agatgcgcat gccccggcac aatctccaca aagctgttgc aaatgccgat aaagggcttc
     5641 ttaaaatctt tggaacattt cacctgaccg gtggcataaa gcaatgaacg gtgcggagcc
     5701 cgctcaaatc cgtctttcac tgtgctgctg cccatgaata cttctcctct atggctaggt
     5761 agtgggttgc aaaaattaaa gtatacgtcc tcatcccttg tcaatcaaat cagcgttttt
     5821 ttcaccacga aggcacggag ggcaccgagg ctcacggcgt ttttatacca ctcctggaag
     5881 catcgggttg aaactcttca agaccggcgc acaggtaaaa atatccgtgc ggcatatctt
     5941 tgtctacttc gccggcgcct gtccttatca ggatgtcttg cgtcatgcgt tgcaaaaaac
     6001 taaagacgta tcccgttcgt tgctgaccag atgttcagca gcctccgagg gacttgatag
     6061 aagacaatac atgccacgga ggaaaggggg agttgagttt aaagagcgaa aaacaggcct
     6121 gtcacagtaa aatccgcaag aaaccgtcct caaaaatcca agcaggccgt gccatgtcca
     6181 aaaaacagta ctgtcctgag tcagtgtgaa ctatccgggc taatccccga ctcagatccg
     6241 tggtaatccg ctgtggtgat tcgtcttaag aacccgggtg caaaaaaaat cgttgatatt
     6301 gagggtgtgg actagtctgc cacatctgaa aaaatgaaag tctgtcatgg atacatcgtt
     6361 tgttcaggaa agcaattccg gtacgatggc agcaatactc aatcagaatt ttcagaagaa
     6421 atttctctat aaagactggc tttgctacta tagagtctca ggtattacac cctgccgtta
     6481 acggactcac tgtcagatcc ggaaaaacgc ttggatttcc gaacgacgtt cgaagtcttc
     6541 tttgaataga aattgtattt caatgttgaa tccgatatca gaagggagcc accgtgtcat
     6601 acgctatttc cgagtaccac gatacagacg ccatttaccg tgatttggac accctcatgg
     6661 ctttgccgcc cgtgacgctc cggcgcgatg ccatgcagca gtggctggag caactgaatc
     6721 agcgctgcgc aaagtcaaaa gcaatgatcg ccgaggcgaa agaatttatt cccggcggcg
     6781 tgcagcataa cctctccttc aactaccctt tccccctcgt catggatcgc gcagaaggtc
     6841 cttacctcta tgacatcgac ggcaaccgct acatcgattt tcttcaggcg ggcggcccta
     6901 ccgtcctcgg ttccaatccg cctgccgtcc tcgaacaggt catcgagctg cttcgccatt
     6961 gcggtccttc caccggtctc ttccacgaat tcgaattgaa aatcgccaaa gaaatcgcaa
     7021 agcacatgcc cgctgtcgaa atgttccgca tgctcggcag tggcacggaa agtgtcatgg
     7081 cggcgatccg tgtcgcccgc ctcgccacgg gcaagaaaaa catcatcaaa atgggcggtg
     7141 cctaccatgg ctggtccgac cagatggtgt acagcctgaa aattcctaaa acccgtcgct
     7201 tcgaggccca cggcatccca cgggcctgcg tgaaatacac caccgaggca gcgaataaca
     7261 acctgaagga tctcgaacgc cgtcttttca aaaatcgctt ccgcggaggt accgccgctg
     7321 tcattgtgga gcctgtcggt cctgaaagcg ggacccgtcc tgtggcgcgt gattacaatg
     7381 ccggtgtgcg cgagctctgt gaccgctatg gcgccctcct cattttcgac gaggtggtga
     7441 ccggcttccg cctcggcatg ggcggtgccc agggctactt caatgtccgc cccgatctca
     7501 ccattttcgg caaggtcgtg gcgggcggtt atcctgcagc gggcggcgtc ggcggcagac
     7561 gtgatctgat ccagcgcatg gccgccggat tggaaagcgg caaaaagcgc gcctacgtgg
     7621 gcggcaccct ggccgccaat ccgctgagtg cggcggcggg ctatttcgcc attcaggaaa
     7681 tggaacgcac caacgcctgt gtcaaggcgg gccaggcagg cgatcgtctg acccggggtc
     7741 tccgcaccat gattgaagaa atgaagctgc cctttgtcac ctacaaccag ggctccatct
     7801 gtcacctcga aaccattggc ggcatgttca tcaagctgga ctatctgcgc atcctgaaag
     7861 tgctcaagga gatcaaggtg cgcaagcatg ccatggaaga atacggcgcc gcttacatgg
     7921 cggagggcat catcacgctt gcgggaagcc gactctatac gagcatggcg gacactgacg
     7981 aggtcatcga tgacgctctg gcacgcttcc gccgtgtctt tgaaaaagca gactgggacg
     8041 gggcgaaagg gaagcagtca tgagcgatat tcacgcagca agaaaagcgg tacgtgccat
     8101 ggggctgcgt ctcttggaaa cgggtctggt tatcggcacc tggggcaacg tcagcgtgcg
     8161 cgccggtggc aaggtggtca tcacaccgag cggagccgac tacaccctgt tacaggaaga
     8221 agacatgcct gtggttgacc tggaaacagg cgcctacgaa ggggcgaagc cgtcgagtga
     8281 aaaagggctt cacctcgaag tctatcgcca gagaaaagaa atcaacgccg tgatccatgt
     8341 ccattcaccc catgcagcca cgctggcggc ggcgcgcaag cccctgccgc ccattctcga
     8401 cgacatcgcc cagctcatcg gtcccggcgt gcgtgttgcc gactatgccc tgcccagcac
     8461 gaagaagatc gtgcgcaaga ccttgcgcgc ccttaaggga cgcatggcgg cgctcatggc
     8521 gaatcacggc gccatctgcc tcggtcgcga tctcgaagaa gcctacctct gctgtcaggt
     8581 gctggagaag ggatgcaagg cttatatcga ggcggagttt ctgggcggtg cgaaaggtat
     8641 caactatttc gaggcggcac tcatgcacca gttttttctc cgcaaatact cgaggcaatg
     8701 gaaggataaa aacccgacat gacccctccg cactatccgg cagactgctg aaacatgaaa
     8761 gcggacaaga catatgttct tgccgtagat ctcggcacct caggctgcaa gactgccctc
     8821 gtctccctcg gcggcgaagt cgctgcgtgg cattttgagc ccgtcgaaac acgcctcttc
     8881 ggcaacggcg gcgcggaaca ggaaccgcag gactggtggg atgctttcct caaaaccgca
     8941 cgggctgttc tcgacagcgc cggagcagcg ccggaatcga ttgcggcggt ctgctgcagc
     9001 actcagggcg aaggtacgat cccggtcgat gctgagggca aggtgctcca tccctgcatc
     9061 ctctggatgg atacccgggg tgcggaatat cttcgggaaa tcaccggaaa aaccctgcgt
     9121 gtggcaggct atggcccgct ccgcctccgg cgctggatcc ggctgaccgg cggtgcgccc
     9181 tcccttaccg ggaaagaccc cgccgcacac atgctcctga tcaaacatgc catgcccgat
     9241 atttaccgaa acaccgccaa gttcctgaat gtccccgact acatgaatct ccggctgacc
     9301 ggacgcattg tcgccacacc tgattccatc ctcacttcct gggtgacaga taaccgtgat
     9361 cccgcccgca tcgtctacca tgacggactc gtccggaaca gccagatcga aaaggacaaa
     9421 ttccccgaga tcgtcccctc cacggaagtg atcggaacgc tcctgcctga agtggcggag
     9481 cagctcggcc tcccttcgac cgtgcccgtt gtcgccggct ccatagacac caccgccgcc
     9541 gccatcggct ccagagccgt ggcggattac gatgcccatc tctacctcgg cacttcttcc
     9601 tggctggcgg cccatgttcc cttcaagaaa acggatatct tcgcttccct cgcttcggtg
     9661 ccctgcgcat tgaaagaccg ctatctcatg atcgcgctgc aggcaacggc gggaggcaac
     9721 ctgacctttc tccgcgacaa gattctctat cataaagatg agcttcttgc ggaggcttcc
     9781 gtacccgatg tgtacgcact catggatcag atagtggaaa gaacgccgcc gggaagcaac
     9841 ggcgtcctct tcacgccctg gagtttcggc gagcgctccc ctgtggaaga tccgctcatc
     9901 cgcgccggca tccacaacct ttctctggaa aactcgcggg aggatattat ccgcgccttt
     9961 ttcgagggta tcgccttcaa caaccgctgg ctcctcaagc ctgtggagcg cttcctgggg
    10021 cggaagctcg aggccatcac cctgacaggc ggcggcgcca attcggatgt gtggtgccag
    10081 atcttcgccg acgtgctcca gcgcccgata cgtcagctcg ccgatcccat acaggtgaac
    10141 gtcctcggtg ccgccttcat cgccttcctc ggtctcggca ggatcacaaa agaggagatc
    10201 ccccgcattg tccggcataa ggcggtctat gaaccccgcg ccgaactgca aactctctac
    10261 aacgacctct tccgggaatt cgttgccctc tacgaaagga acaaagccat ccaccaccgg
    10321 ctcaaccgga agcgcacccg ctcataacgc tgaagagccg gcaggtgata tgctcctcaa
    10381 ccgttaattg gcaaagaagg gttcaccgca gagacacgga gagcaccgag gcacacagag
    10441 aattttaacc acgaagaaac gaaggttcac gaagaaaaga taagtagtgc tccagccagt
    10501 tacaaagttc cctgctatag cccggctttc aactcacccc ggcgtcttcc cgtttttttg
    10561 cagctcagca cacctatcga ggagttaagc cacaacaatg caaaaaaatc cgtgaataaa
    10621 tgcaggaaca gattactaaa aaagcactgg cagccacctg ttatttgaga taaaaagcct
    10681 ctctctatta gacttaaata gtgcattctc tcagacgggt tagttcagca cgcccatttc
    10741 gaagccccgg ttctttttga taaggccgaa gacattgacc acacttaacg tggagatatc
    10801 aacatgaatt caggacatcc gatggtgaag ggattggtgt gcgtcatatg gacatgcgcc
    10861 cttactttgg tggcaatggt accggtggcg cgggcaggcg aatcggatga tgcgggttcg
    10921 ctggcactgc gtccggcaaa agtggagcgc attctcctcg gcggcgtggt cggcgatcgc
    10981 gtacaggcca atatcgacgc ctggctgctc agtgcgccgc ttgccaaccc cggcatgatc
    11041 gagatgtttc gtgtgcgcga tcgcaagccg gtgcccgaac tggtgccgtg ggctggcgaa
    11101 ttcatcggga agtacctcat cagtgcgatc caggcccggc gcatgacgga tgatgatcgg
    11161 ctcgactcct tcctgcggga agtgatcccg gagttcattt cgacccaggc cgaagacggc
    11221 tatctggggc ccttccgtaa agaggaacgg ctgctgggac attgggatct ctggggccac
    11281 taccacgcca tcctggcatt gctcatgtgg tatgaagata cgggtgaccc cgccgctctg
    11341 gcttgtgcac gccgcacagg cgacctcatc tgctcgctct atttaaacac aaaccgcaga
    11401 ccgaagcatg ccggtgcgga tgaaatgaac ctcgccatca tccacgggct cggcaggctt
    11461 taccg
//
LOCUS       DAOPWB010000098        11416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5789_length_11416_cov_4.953877, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000098 DAOPWB010000000
VERSION     DAOPWB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5789_length_11416_cov_4.953877"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PKN92_09310"
     CDS             <1..766
                     /locus_tag="PKN92_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ49030.1"
                     /translation="GDVWIGHNVSIAFYDQHQETLASDMDVLSLMQQSKPEWSQQECR
                     SYLGRFLFTGEDVFKPASILSGGERSRLALAQMIASEANLLLLDEPTNHLDIASREAL
                     ENGLANYTGTLVVVSHDRTLIDRLATRLLVVQDGKVESFAGNFSEWRQRQLEKSSAAE
                     TKTDKNEDERKKKNLNEREQKKAQERKQRRDQRALQKIEEEIAVYECTIEAFHQRFAA
                     LDPADFEEAQKLQKEYDALKATVERLYEEWAAMAEE"
     gene            1017..1145
                     /locus_tag="PKN92_09315"
     CDS             1017..1145
                     /locus_tag="PKN92_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49031.1"
                     /translation="MKHTKPVTRKPELSQISNFQVFKDFLLKSLDQLIEFVFILTR"
     gene            1163..1378
                     /locus_tag="PKN92_09320"
     CDS             1163..1378
                     /locus_tag="PKN92_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49032.1"
                     /translation="MRKLTHIKVLTETPEQAQISNFQVKLEFTSSVIDRLLLADRQVA
                     WKIIPGQGTDTDTDITGTDIPDNITRL"
     gene            1547..2344
                     /locus_tag="PKN92_09325"
     CDS             1547..2344
                     /locus_tag="PKN92_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNZ49033.1"
                     /translation="MKPLGTVEGFLFDMDGTIYMGPHPIPGAAAFIAWLQENEVPFLF
                     LTNNPTADKKRYRSKLAGMDIHVPEEQILTSGEATAVYLMTETPYRRIFALGTPSFEN
                     ELVQAGLLLEERDPDAVVLSFDKTLTYAKLERAALLLQKGIPYIATNPDRVCPTEYGP
                     IPDCGAMAALLESATGRSPKFIGKPEVSFARMAQKKLGTPMHATAMVGDRLYTDLEMA
                     RLAGMVGILVLSGETTHEDLREAAHAPDFTFESVRELHAAYQESRRL"
     gene            complement(2339..3091)
                     /locus_tag="PKN92_09330"
     CDS             complement(2339..3091)
                     /locus_tag="PKN92_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNZ49034.1"
                     /translation="MEHPLSRTEALIGEEALKQLRQAAVTVLGIGGVGSYAVEALARC
                     GIGHLCLVDADKISVTNINRQIHATPATVGQIKVEAMAARIREIDPSICVTVHCTRIT
                     AENLKELLSPAAHYVIDAVDTISVKIAAAEYCWRNGIPLISCMGAGNKMDPAAFTVAD
                     IFQTRQCPVAKVLRRALKKRGVPSLKVVYSPEEPVHVQAVCDVDAGNQEERALQKRCV
                     PASIAFVPSVAGLILAGEAVRDLIGFVQKQHL"
     gene            3287..3568
                     /locus_tag="PKN92_09335"
     CDS             3287..3568
                     /locus_tag="PKN92_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49035.1"
                     /translation="MAESYNIQQISRLAANFVVKQRGNWTHEQWVHFCSSIMDLQIPL
                     NENDSRHLGLLLESLKALYLSVPKKIKTAKKKTAAKAKAKPKTPPKASA"
     gene            3740..4546
                     /gene="cysE"
                     /locus_tag="PKN92_09340"
     CDS             3740..4546
                     /gene="cysE"
                     /locus_tag="PKN92_09340"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNZ49036.1"
                     /translation="MESDKRDRLWDLIREETAEGAWREPMLSSFLYSSILNHKSFEAA
                     LGFQLASKLESVTLPALTLRDLIEEAFDAEPRLAEYARADLLAVHERDPACRKYSQPL
                     LYFKGYLSIQAQRVANHYWHQDRMHLALFLQSRISDIFGVDIHPAASLGKGCFIDHAT
                     GVVIGETAVVEENVSMLHGVTLGGTGKERGDRHPKIRKGVLISTGARILGNIEVGEGA
                     KVAACAVVLENVPPHTTVAGIPARPVGATSYAQPSLEMDHKFKEAESTVR"
     gene            complement(4572..5471)
                     /locus_tag="PKN92_09345"
     CDS             complement(4572..5471)
                     /locus_tag="PKN92_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNZ49037.1"
                     /translation="MATNILNLPEAEYMLSGNRACAGCSIGIFFRLITKAMEGKAVFV
                     VPASCFTVFCGMYPSSSVKVPIVNSVFPGTAACASGIAASLERQGRTDLTVVAMAGDG
                     GTADIGIQALSGAAERGANMLFICYDNEAYMNTGTQRSGMTPLGARTTTTPAIGKIQP
                     PKDMPAILEAHGIPYIATASPGFPADLYDKVVKARNIRGTRYMHLNAPCPAGWHYDAK
                     DTVTLAKMAVDTGAFILSEIEDGVFRLTGRSLSLAQTGKRRSIEEFIRPQGRFGRASE
                     EDVAALQQWVDRRWEKAVRRHQD"
     gene            complement(5498..6712)
                     /locus_tag="PKN92_09350"
     CDS             complement(5498..6712)
                     /locus_tag="PKN92_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNZ49038.1"
                     /translation="MSNAASVSVITGNKAAAEAARLARVQVVAAYPITPQSQVVETLA
                     SYVETGKLDAEFITVESEHSAMGVCIGASIAGSRVFTASSANGLAYMCEQLSWAAGSR
                     LPIVMGCANRALSAPWNVLNDQQDAMSQRDQGWIQLYCRDNQEIFDTFLQAFRLAEEV
                     RLPVMVCYDGFILSHTVMPVSIPTQEEVDAFLPPFQGRTIVDLEDPRNINPVTLANPR
                     NNADGTICHGYMEFRALMQQAHLASLAAIDRVDQEFASRFGRATGGVTWSHALEDADV
                     ILLAAGSLANEATVASDYLREQGVRAGVLGLRSYRPFPAARVAEQLSEARLVTVFDKS
                     LSYGYGGPIFTDTQAALFSARKRPEMHSYIAGLGGRDVKARELADAVSRSLDWVAQEN
                     GQKEAEWLNLQL"
     gene            complement(6705..7037)
                     /locus_tag="PKN92_09355"
     CDS             complement(6705..7037)
                     /locus_tag="PKN92_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ49039.1"
                     /translation="MVETRHCQQETAMSWPCVGEGGRTGDWRVAKPVLDPALCVAVKK
                     GSIACMLCWIFCPDNVITRTVPPQIDLEYCKGCGICMEVCPSKAIVMEPENVEQPALT
                     VEGTCCDV"
     gene            complement(7059..7649)
                     /locus_tag="PKN92_09360"
     CDS             complement(7059..7649)
                     /locus_tag="PKN92_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNZ49040.1"
                     /translation="MTETIYEIRWHGRGGQGAVTSANMLADAGYRAGFKGVMSMPTFG
                     AERRGAPVTASTRLSREVLRFFSQVESPDMVIVLDDSLLPAPATTQGLASGGFLLINS
                     AADPVSLDSGKGFRVAVTDANEIAREAGLVISGAPMVNTAMLGAIARATGLIDLEHIE
                     TAMEGVFSKAALAKNVEAAHQAYERTVCLYAVESVK"
     gene            complement(8026..8862)
                     /locus_tag="PKN92_09365"
     CDS             complement(8026..8862)
                     /locus_tag="PKN92_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ49041.1"
                     /translation="MTYVDLHLHSNCSDGADSPEVVVERAAALGIAAIALADHDTVAG
                     VAAARKAAEAQGMGFLDAVEVSASLEGRELHILAYGVHIQSPVLLNMLQNLREMRQKR
                     IERILGRLESSGIPVCDALAERKTDFDMTGRMHVAVLLHRMGVVPTVQKAFEHYLNRR
                     RLAYVPKELPEAADVIRTIHDAHGLAFLAHPGLGKNTKGRLTKILELPFDGIEVWHTS
                     HTPDMCVRFSAIAQEKQLLCSGGSDCHGAVKGEHPTMGRIKTPLEQYHRICSALSSFS
                     SC"
     gene            complement(8889..9368)
                     /gene="nusB"
                     /locus_tag="PKN92_09370"
     CDS             complement(8889..9368)
                     /gene="nusB"
                     /locus_tag="PKN92_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNZ49042.1"
                     /translation="MSRFKPQQKGRQLAFQFIFGIEYMPSEWTAALAQFWEMNPAALG
                     RDYPAGERDADVTAVRADERAFSEKLICGVMEHKKELDSAIVDALDNWAPARVGRMEW
                     AILRLAAYELLYDHDTPHRVVLSEAMRLGDLYGEENTARFVNGILNRIIDKRETGES"
     gene            complement(9370..9834)
                     /gene="ribE"
                     /locus_tag="PKN92_09375"
     CDS             complement(9370..9834)
                     /gene="ribE"
                     /locus_tag="PKN92_09375"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNZ49043.1"
                     /translation="MATMLQGDFLGHDKKIGIVVSRFNEFISSKLLAGALDALQRHGV
                     ADENITVAWVPGAFEVPLITKKMAQSGKYDAVIALGCIIRGATPHFDYVANEAAKGIG
                     HVMLDTGVPVLFGILTTENIEQAIERAGTKAGNKGADAAMGALEMINLLEQI"
     gene            complement(9853..11049)
                     /locus_tag="PKN92_09380"
     CDS             complement(9853..11049)
                     /locus_tag="PKN92_09380"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNZ49044.1"
                     /translation="MLTSIPEILEELRLGRMIILVDDEDRENEGDLVAAAESITPEII
                     NFMATHGKGLICMPMEDADLKRLDLPPMVMENTTKLGTAFHVSFEARDGITTGISAHD
                     RAHSIRVAARPESLAGDLVRPGHVFPLRARPGGVLVRAGQTEGSIDLMRLAGLRRASV
                     ICEIMKNDGTMARMPDLEIFAQEHNLKICSTADIIRYRRQNEKLVNRVAQVQMPTEYG
                     DFSLIAYENNIDAYQHLALIKGDVTGKEDVLVRVHSECFTGDVFASRRCDCGSQLHEA
                     MRMINEAGCGVLVYMRQEGRGIGLINKLKAYELQEQGMDTVEANLHLGFDADPREYGL
                     GAQILQDLGVTSMRLITNNPVKRAGLEGYGLTVSGRVPLEVAACATNRHYLETKKEKM
                     GHFLEI"
     gene            complement(11058..>11416)
                     /locus_tag="PKN92_09385"
     CDS             complement(11058..>11416)
                     /locus_tag="PKN92_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNZ49045.1"
                     /translation="GHFVLGHVDGVGRVLSVKNQGEFSVWTFQAPDDVAQYLAPKGSV
                     AIDGISLTVVNPEKDTFGVAVIPVTIKHTTLSLRRPGDSVNMEADVIGKHVRHFMKND
                     ARQGITPELLARNGFL"
BASE COUNT         2803 a         3146 c         2805 g         2662 t
ORIGIN      
        1 tggcgacgta tggatcggac acaacgtttc catcgcattt tatgaccagc atcaggagac
       61 cctggcgtca gatatggatg tgctcagtct tatgcagcaa tcaaagcctg aatggtcaca
      121 gcaggaatgc cgcagctatc tgggacgctt tctttttaca ggcgaagatg ttttcaagcc
      181 tgcctccata ttaagcggcg gtgaaagaag ccgccttgcg ctggcgcaga tgattgcatc
      241 ggaagccaat cttttattgc tggacgagcc taccaatcat ctggatatcg catccagaga
      301 agcgctggaa aacggactcg ccaattatac aggcacactc gtcgtggtaa gccacgaccg
      361 cacactgatc gaccgtctgg caacgcgcct tcttgtggtg caggatggca aggtggaatc
      421 tttcgccgga aacttttcag agtggcggca gcggcagctg gaaaagagca gtgcagcgga
      481 aacaaaaaca gacaaaaatg aagacgagcg gaaaaagaaa aatctgaatg agcgggagca
      541 aaaaaaggca caggaacgaa agcagcggcg cgatcagcgg gctctgcaga aaatagaaga
      601 agaaattgcc gtgtacgagt gcaccattga ggcttttcac cagcgttttg ccgcactgga
      661 tcctgctgac tttgaagagg cgcagaaact gcagaaagaa tatgatgccc tgaaagcgac
      721 ggtggaacgg ctttacgaag aatgggcagc catggcagaa gagtagcgca gcgctacgcc
      781 cggactgtcc ttcctgtttt ttaaatggac tcacccgggg cagctatgct atagttatgt
      841 gcaaatgaaa gtcgctgaag agaaaacact tgacaaaatt attcatttgt ggtattaata
      901 cggtatcaga ttttgtttgg agttagtgga aagggcggtc tgtcgttata gacaggctcc
      961 tcaaaagcgg gtttgccagg gcagatccat tacgagagta ctcaggagca gccgatatga
     1021 aacacaccaa accggttacc cgtaagccgg aactctcaca gatatccaat tttcaggtat
     1081 ttaaggattt cctgttgaag tctctggatc aactcataga atttgttttt attctgaccc
     1141 gataatgcag gagtctctgg ctatgcgtaa acttacacac atcaaagttt taacagaaac
     1201 acccgaacag gcgcagatca gcaactttca ggtgaaactg gagttcacct cctccgtcat
     1261 tgaccgcctg cttcttgccg accgtcaggt cgcctggaag atcatccccg gtcaaggcac
     1321 cgatacggat acggatatca ccgggacaga tattcccgac aatatcaccc gcctttaatc
     1381 tgaaccattt tttcgttcct ttttgtgttg cagcgcatgt acccatactc tcgggtgcat
     1441 gcgctggttt tttgtttttt tcacggacac agaaagtgcc gcgctttatg catgttgaca
     1501 tgcatgcctt ttcaaaaaac tctaccgcag gatattggaa cactctatga aaccactcgg
     1561 aactgttgag ggctttctct ttgatatgga tggaacgatt tacatgggtc cccatccgat
     1621 tccgggcgca gcagcgttta ttgcctggct gcaggaaaat gaagtgcctt ttctctttct
     1681 gaccaacaac ccgacagcag ataaaaagcg ctaccgctcc aaactggcgg gtatggacat
     1741 tcatgtaccc gaagaacaaa tcctgacatc aggcgaagca acggcggtgt atttaatgac
     1801 ggaaacaccg tatcgtcgca tcttcgcgct gggaacccct tctttcgaaa acgagctggt
     1861 tcaggcaggg cttctgctgg aagaaaggga tcccgatgct gtcgtccttt cctttgataa
     1921 aacattgacc tatgccaaac tggaacgggc agcactattg cttcaaaaag gcattcctta
     1981 tattgccacc aatccggatc gtgtctgccc gacggaatac ggtcccattc cggactgcgg
     2041 tgccatggcg gcacttttgg aatctgcaac agggcgcagc ccgaaattta tcggcaagcc
     2101 ggaagtatct tttgctcgca tggcgcagaa aaaactggga acgcccatgc atgccaccgc
     2161 catggtaggc gatcgactgt ataccgatct ggaaatggca cgtctggcgg gtatggtcgg
     2221 cattctggtg ctgtctggag aaacaaccca tgaagacctc cgggaggcag cgcacgctcc
     2281 cgatttcacc ttcgaaagtg tacgggaact ccatgctgct tatcaggagt cccggcgttt
     2341 ataagtgctg cttttgcaca aatccgatca gatccctgac agcctcgccc gccagaatca
     2401 gcccggcaac tgatggaaca aaagcaatgc tggcaggaac gcaccgcttt tgcaacgccc
     2461 tttcctcctg gtttccggca tccacatcac acactgcctg cacatgcaca ggctcctcag
     2521 gcgagtaaac cacctttaag gagggaacgc ctctcttttt caacgcgcga cgcagtactt
     2581 tcgctaccgg acattgccgg gtctggaaaa tatccgccac agtaaatgct gcaggatcca
     2641 ttttgttgcc ggcacccatg cagctgatca ggggaatgcc atttctccag caatattcag
     2701 ctgccgcaat ttttacggaa atagtgtcca cagcgtcaat cacataatga gcagcaggcg
     2761 aaagcagttc tttcaggttc tccgctgtaa tccgggtaca gtgaacagta acacatatgg
     2821 aaggatcaat ctcccggatg cgggctgcca tggcttccac tttgatctgc cctaccgtcg
     2881 ccggtgtcgc atggatctga cggttgatat tggtaacact gattttatcc gcatcgacaa
     2941 ggcacaaatg cccgatgccg catcgagcca gtgcctcaac cgcataactt cccacgccgc
     3001 ctataccaag aacggtcaca gccgcctgac gcagctgttt caaagcctct tctccgatca
     3061 gggcttcagt cctcgacaag ggatgttcca tcatagcgtg cctttctccc gtgcattttg
     3121 attattctct actttatcag agaaagtctt ttcgggtgca ttataatata cgggaatagg
     3181 aagcatgtcc caatcagtta cgtttagcag ggatacagca ctacccacct ttgaaatgaa
     3241 tacgattgca cagggataag atatcccaat caggagaaag catgccatgg cagaaagtta
     3301 taacatacag cagatttccc gacttgctgc caactttgtt gtaaagcaac gcggaaactg
     3361 gacacatgag caatgggtgc atttctgttc ttcaatcatg gatctgcaaa ttccattgaa
     3421 tgaaaatgac agtcgccatc ttggtctcct ccttgaaagc ctgaaagcgc tttatctgtc
     3481 tgtccccaaa aagatcaaaa cagcaaagaa aaaaacagcg gcaaaagcca aagcaaaacc
     3541 aaaaacacct ccgaaagcct ctgcctgatc attttataat ccgtctctct tttatcagga
     3601 aggcgcagtg gcttacaata cgggggcggg catacctgga cagcggaaga cgggacgtct
     3661 gttttttaaa aaaaggcttt tcaccggatt cttatttcgg ctgccataac tgaagtgctc
     3721 cttatttaaa gggatttcca tggaatcaga taaaagagat cgtctctggg atttaatccg
     3781 ggaggagact gctgagggcg cctggcgcga gccgatgctg tccagttttt tatattccag
     3841 cattttaaac cacaaaagtt ttgaggctgc actggggttt cagctggcaa gcaagctgga
     3901 gagtgtcacc ttgcctgcgc tgaccctccg tgatttgatt gaagaggctt ttgatgcgga
     3961 accacgcctg gcggagtatg caagggctga tcttctggca gtgcatgaac gtgatcctgc
     4021 ttgccggaaa tactctcaac cgctcctcta ttttaaaggc tacctctcca ttcaggcgca
     4081 gcgtgtagcg aatcattact ggcatcagga caggatgcat cttgcgcttt ttctgcagag
     4141 ccgcatttct gatattttcg gtgtagatat ccacccggcc gccagtcttg gaaaaggatg
     4201 ctttatcgac catgccacag gtgttgtgat tggcgaaaca gcagtcgtgg aagaaaacgt
     4261 gtccatgctt cacggggtta ccctgggcgg caccggcaaa gaacggggtg accgacaccc
     4321 caagatccgc aaaggcgttc ttatttcaac aggcgcacgt attctcggaa atattgaagt
     4381 aggcgaaggt gctaaagtgg cggcctgtgc cgtggtgctt gaaaatgttc cgccgcacac
     4441 aaccgttgca ggcattccgg cacgtcccgt cggcgccacg tcctacgccc agccttcgct
     4501 ggagatggac cacaaattta aagaagccga aagcactgtg cgctgatttt ctcttcggaa
     4561 gcgccttcag atcagtcctg atgccggcga actgcctttt cccagcggcg gtccacccat
     4621 tgctgcaatg ctgccacatc ttcttccgag gcgcgcccga aacgtccctg cggtcgaata
     4681 aactcctcta tggacctgcg cttcccggtt tgtgcaagac tcaggcttcg cccggtaaga
     4741 cggaaaacgc cgtcttcgat ttcggacaga ataaatgctc cagtatcaac ggccattttt
     4801 gccagagtga ccgtgtcttt tgcatcataa tgccacccgg cgggacaggg tgcattcaaa
     4861 tgcatataac gggtaccccg gatatttctt gccttgacta ccttgtcgta caggtcggca
     4921 ggaaatccgg ggctcgctgt agcaatataa ggaatgccat gggcttccag aatggcaggc
     4981 atatctttgg gcggttgaat ctttccaatg gcaggcgtcg ttgtagtgcg tgcacccaag
     5041 ggggtcatcc cgctcctctg ggtgccggta ttcatgtagg cctcattgtc atagcagata
     5101 aaaagcatat tggcgcctct ttcggcagcg ccgctcagtg cctgaattcc aatgtccgcc
     5161 gtaccgccat cacctgccat ggcaaccaca gtcagatccg ttctcccctg tctttccaga
     5221 gatgcggcaa taccggaagc acaggccgcc gtgccgggga acacgctgtt tacaatgggc
     5281 acttttaccg acgagctggg atacatgccg caaaacaccg taaaacagct ggcaggaact
     5341 acaaatactg ctttcccttc catcgctttg gttatgagtc tgaaaaaaat accgatactg
     5401 catcctgcac aggcacggtt gccggaaagc atatattcgg cttcaggtaa atttaaaata
     5461 ttcgtcgcca tggttttctc caatcaggaa ccaaaggtta gagctgcaaa ttgagccatt
     5521 ccgcctcttt ctgtccgttt tcctgcgcaa cccaatcaag gcttcggctc actgcatcgg
     5581 caagctcccg ggctttaaca tcacgaccgc ccagacctgc aatatagctg tgcatttcag
     5641 gtcttttccg ggcactgaac aatgccgcct gcgtatctgt aaaaatcggg ccgccgtagc
     5701 cgtagctcag gcttttatca aacacggtta caagccgtgc ctcgctcaac tgctctgcca
     5761 cgcgggcggc agggaaaggc ctataagagc gcagtccgag cacaccggcg cgcacaccct
     5821 gttcccggag atagtcgctg gcaactgtgg cttcgttggc aaggctgccc gcagccaaca
     5881 gaatcacatc ggcgtcttcc agtgcgtggg accaggtaac cccgccggtt gcccgaccga
     5941 accgggacgc aaattcctga tctaccctgt ctattgccgc cagagacgca agatgcgcct
     6001 gctgcatgag ggcgcggaat tccatatacc catggcaaat ggtgccgtcc gcattgttcc
     6061 ggggatttgc cagagtaacc ggattgatgt tgcgcggatc ctccagatca acgattgttc
     6121 ttccttgaaa cggagggaga aaagcatcca cttcttcctg agtcggaata gagacaggca
     6181 taacggtatg ggacagaata aaaccgtcat aacagaccat cacaggaagt cttacttcct
     6241 ccgccaggcg aaacgcctga agaaatgtat cgaaaatttc ctggttgtcg cggcagtaaa
     6301 gctgaatcca gccctgatcc cgctgagaca tggcatcctg ctgatcgttc aacacattcc
     6361 agggtgcgga cagcgcacgg ttggcacagc ccatgacgat aggaagccgg gagcccgcag
     6421 cccaggaaag ctgctcgcac atatacgcca ggccgttggc actactggcg gtaaaaacac
     6481 gggagcctgc aatagaagcc ccgatgcata cacccatggc actatgttcg ctttccacgg
     6541 taataaattc agcatcaagt ttgcccgtct ccacatagga agcgagggtc tcaaccacct
     6601 gcgactgagg tgttatgggg tacgctgcca ccacctgcac tctggcaagc cgtgcggctt
     6661 cggctgcagc tttattgcct gttatcactg aaacagatgc agcattagac atcgcaacat
     6721 gttccttcca cggtaagtgc cggctgttcc acgttctccg gttccatgac aatcgcctta
     6781 ctggggcata cttccatgca aataccgcac cctttgcaat attcaagatc gatttgtggc
     6841 ggaactgtac gggtgatgac attatccgga cagaagatcc agcataacat gcacgcaata
     6901 ctgccctttt taacggcaac gcatagcgcc ggatcgagaa ccggtttggc aacccgccag
     6961 tctcctgtac gcccgccctc ccctacgcaa ggccaggaca tagcggtttc ctgctggcag
     7021 tgtcgtgttt caaccattgt ccaaactcct ttcagggatt atttcacgct ttcaacggca
     7081 taaaggcaca ccgtccgttc ataggcctga tgagctgcct ctacgttttt agccaaagca
     7141 gctttggaaa aaacgccttc cattgccgtt tctatatgtt ccagatcgat cagcccggtc
     7201 gcccgggcaa tggcacccag catggctgta ttgaccatgg gcgccccgga aataaccaac
     7261 cccgcttccc gtgcgatctc attagcatcg gtaacggcta cacgaaagcc ctttcccgaa
     7321 tcgagagaga ccgggtcagc ggcactgtta atcagcagaa aaccgccaga cgcaagtccc
     7381 tgggttgttg ccggtgcggg aagaaggctg tcgtcaagaa cgatcaccat atccggactt
     7441 tccacttgag aaaaaaaacg gagcacttcg cgggaaagtc gtgttgatgc tgttaccgga
     7501 gcaccacgcc gctctgcacc aaaggtcggc atggacatca cccctttaaa accggctcga
     7561 tagcctgcat cagcaagcat attcgccgag gtaacagcac cctgcccgcc acgtccgtgc
     7621 catcggattt catagattgt ttctgtcatt tcagcctctc atgatgacgg cggatgatgc
     7681 cgtcccttag atctttcaat ctccgatcgc gtgctaccag ctcagaatag cagtgaacac
     7741 gggccttgaa acggtcgggt tcctctgcaa aaaggagccg gatttcttaa atcagtcatg
     7801 cagaaagagc ataaattcgc ctttactata ccatatacgg caacaggcat gccacgcccg
     7861 gacgaaaaac ggtgtaaaat ctgccgtctt tccttctatc tgtgcttttt ctaagaaatt
     7921 aaaagcatag actccaagtc gtttgataac cgacagcgca tttccataaa ggcatatttt
     7981 tcttggtttc acaacaacag aaatcgtctg gcgggactaa aggggtcagc aggatgaaaa
     8041 ggaagataag gcactgcaga tacggtgata ctgctccaga ggcgttttga tcctacccat
     8101 ggtggggtgt tcccctttca ctgccccgtg acaatcgctg ccgccgctgc aaagcaattg
     8161 tttttcctgt gcaattgctg aaaaccgcac gcacatgtcc ggtgtgtggc ttgtatgcca
     8221 gacttcaatg ccgtcaaagg gcagttcaag tattttggtg aggcgtcctt tcgtgttctt
     8281 ccccaacccc ggatgggcaa gaaaagccag cccgtgcgca tcatgaatgg tacggataac
     8341 atcagctgcc tcgggcaatt ctttgggcac ataggcgagg cgccgccggt ttaaatagtg
     8401 ttcaaacgct ttttggacgg ttggtactac ccccatccga tgcagcagca ccgccacgtg
     8461 catgcgtccc gtcatatcaa aatcggtttt tctttccgcc aatgcatcgc aaacgggaat
     8521 tccggaagat tccagacgac cgagaatgcg ctcaatccgt ttctgccgca tctcccgaag
     8581 gttctgcagc atattcagaa gcaccggcga ctgaatatgc acgccgtatg ccaggatatg
     8641 cagttcacga ccttccaaag aagcgctgac ctcaacggca tccagaaagc ccatgccctg
     8701 tgcttctgcg gctttgcggg cagccgccac tcccgccaca gtatcatgat ccgcaagagc
     8761 aatggcagca attcccaatg ccgccgcacg ctccaccacc acttccgggg aatcagcacc
     8821 gtcggagcag tttgaatgca ggtgaaggtc aacataggtc atggcaaatt cggaagagcc
     8881 ctggcagctc aggattctcc tgtctcccgt ttatcgataa tccggttcag tatgccgttg
     8941 acaaaacggg cggtattctc ctctccgtag agatcgccca ggcgcatcgc ctcggacaga
     9001 acaacacggt gcggtgtgtc gtggtcatag agcagctcat aggcagccag ccggagaatt
     9061 gcccactcca tacgccctac ccgcgctggc gcccaattgt ccagcgcatc gacaatagcg
     9121 ctgtccagct cctttttatg ctccatgacc ccgcatatga gtttttctga aaatgccctt
     9181 tcatcggctc ggacagcggt tacatcagcg tcccgctctc ccgcaggata gtctctgcca
     9241 agcgctgcag ggttcatctc ccaaaactgt gccagcgctg cagtccattc tgagggcatg
     9301 tattcgatgc cgaaaataaa ctggaacgcc agctggcggc ctttctgttg cggcttgaaa
     9361 cggctcactt caaatctgct ccagcaggtt gatcatttcc agcgcaccca tggcagcatc
     9421 agcaccttta ttgcccgctt tggtgcctgc ccgttcaatc gcctgctcaa tgttttctgt
     9481 cgtcaggatg ccgaagagca ccggcacgcc tgtatcaagc atgacatgcc cgatcccttt
     9541 ggcggcttca ttggcaacat agtcgaaatg aggcgtagcg ccgcggataa tgcagcccag
     9601 agcaatcacg gcatcatact tgccggattg cgccattttt ttggtgatca ggggcacctc
     9661 aaaggcaccg ggcacccagg cgacagtaat attttcatct gcgacgccgt gccgctgcag
     9721 cgcatccaaa gcgcctgcca gcagttttga tgaaatgaat tcattaaacc gcgacacaac
     9781 aatcccaatt tttttgtcgt gaccgagaaa atcaccttgc agcatggtcg ccataatgca
     9841 gactcctttt ttttagattt ccagaaaatg acccattttt tcttttttgg tttccaaata
     9901 atgcctgttt gtggcacagg cggcaacttc aagagggacg cggccggaaa cagtcaagcc
     9961 gtacccttcc agtccggcac gttttaccgg attatttgta atgaggcgca tgctggtaac
    10021 tcccagatcc tgcagaatct gggcacccag accatattcg cggggatcag catcaaaacc
    10081 cagatgcaga ttcgcctcca cggtatccat gccctgctcc tgcagttcat aggccttcaa
    10141 tttattgatc agtccgatac ccctgccctc ctggcgcata tataccagca cgccacaccc
    10201 cgcctcgtta atcatacgca tcgcttcatg aagctgactt ccgcaatcac agcgccggga
    10261 agcaaagaca tcaccggtga agcattcgga atgaacacga accagaacat cctctttgcc
    10321 cgtcacgtcc ccttttataa gcgccagatg ctgataggca tcaatattgt tttcataagc
    10381 aatcaatgaa aagtcgccgt attcagtcgg catttgaacc tgtgcaaccc ggttcaccag
    10441 tttttcattc tgtcggcgat agcgaataat gtcggcggta gagcaaatct tcaggttgtg
    10501 ttcctgcgca aaaatttcca gatcgggcat gcgcgccatg gtgccgtcat ttttcatgat
    10561 ttcgcagata acggaagcgc ggcgcaggcc ggcaaggcgc atgagatcaa tggacccttc
    10621 cgtctgcccg gcacgaacaa gcactccccc gggccgtgcc cgcaaaggga agacatgccc
    10681 gggccgcaca agatcaccgg caagcgattc cggtctggcg gcaacgcgaa tggaatgagc
    10741 acggtcatgg gcgctgattc cggtggtaat cccgtcgcgc gcctcaaaag acacatggaa
    10801 agctgtgccc agtttcgtgg tgttttccat caccatcggc ggcagatcca gtcttttcag
    10861 atctgcatct tccatgggca tgcatatgag ccctttgccg tgggttgcca taaagttaat
    10921 aatttcggga gtaatcgatt ctgccgcggc aaccagatcc ccttcattct cccgatcttc
    10981 atcgtctacg agaatgatca tcctgcccag acgcaattcc tcaagtattt cagggataga
    11041 ggtaagcata gcaatcctta taaaaaccca ttgcgcgcca gcagttcggg ggtaatgccc
    11101 tgtctggcgt cgtttttcat aaaatgccgg acatgctttc cgatcacgtc cgcttccata
    11161 ttaacgctgt cacccggtcg gcggagagac agcgtggtat gtttaatagt taccggaatc
    11221 acagcgaccc cgaacgtgtc tttttcagga ttgaccacag tcaggctgat cccgtcaatt
    11281 gcaacagatc ctttgggagc gaggtactgt gccacgtcat cgggtgcctg aaaagtccag
    11341 acggagaatt cgccctgatt tttgacggac agtacccgcc cgacgccgtc aacatgccca
    11401 agcacaaaat gccctc
//
LOCUS       DAOPWB010000099        11380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5802_length_11380_cov_5.735276, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000099 DAOPWB010000000
VERSION     DAOPWB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11380
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5802_length_11380_cov_5.735276"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKN92_09390"
     CDS             complement(<1..341)
                     /locus_tag="PKN92_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49046.1"
                     /translation="MVILTVVMLVASASATPPSLEAFLDTIPASEPFSQWLTESGEQY
                     PVFDDMPSQASLPDPLLPIVDGKIEAVTTVEAWRKERARLLQVMHHWFLGTVPETPKN
                     MQATLLSEKNT"
     gene            complement(411..1295)
                     /gene="lgt"
                     /locus_tag="PKN92_09395"
     CDS             complement(411..1295)
                     /gene="lgt"
                     /locus_tag="PKN92_09395"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020152174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNZ49047.1"
                     /translation="MTTNTGYWVNYLDPKLIHLWGNMGVSYYGLSYVLAFVLGVCIHR
                     LMISRRRSPFGREEEEVLLYALVLGVLVGARVGYCVLYALPELIARPFFLFEVWHGGM
                     SFHGGLLGVIIACFWVARKTKVSFFQIGDLITPIAPLGLFLGRIANFINGELWGKITT
                     VPWAVVFPRSDPGVALEFIQPRHPSQLYEAILEGLVLFLILQWRFWKTTAWRFPGRLC
                     GEFLFFYAIFRIFCEHFREPDASLFFGVSRGTFYSIFILLTGVFFWVRSLRLGEKEYP
                     KQEMVQPGTGPVKSKKKK"
     gene            1625..3412
                     /gene="aspS"
                     /locus_tag="PKN92_09400"
     CDS             1625..3412
                     /gene="aspS"
                     /locus_tag="PKN92_09400"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006138760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNZ49048.1"
                     /translation="MHPYRTHNCGELRQKDSGNTVKLSGWVHRKRDHGGVVFIDLRDH
                     YGLTQIVINPDTPFFSDAEKIRNESVITVVGEVVKRTDETVNANMATGEIEVVAADMT
                     VESAVDETLPFTVNQELDCPEETRLTYRFLDLRRERMHQNILLRSKATALVREHLVAK
                     GFVEYHTPILTSSSPEGARDYLVPSRLYPGQFYALPQAPQQFKQLLMVSGFDRYFQIA
                     PCFRDEDSRADRSPGEFYQIDMEMSFITQDDLFAELEELMVRLFKELSTKEIAAETFP
                     RIAYRDAINWYGTDKPDIRYDMKMVDCTDIFQNCELKVFSHQAAAGGVIKVLNAKDAA
                     QRPRKFFDDMERYARGEGAKGLAWLALKEGEVKGPIAKFISEDEMKQLFERTDAVEGD
                     VLFFGAGNADETRELMGKVRQMLARQLELVDARKVAFCWIVDFPMFERNPDTGQIEFS
                     HNPFSMPQGGMDSLLNKDPLDILAYQYDIVCNGIELSSGAIRNHRPDIMLKAFEIAGY
                     PESEVKTKFPALWKAFHFGAPPHGGIAPGLDRIVMLLADEPNIREIIAFPLNQKAQDL
                     LMGAPNFVTQAQLDELSLEIKLPADTDAS"
     gene            complement(3489..5216)
                     /locus_tag="PKN92_09405"
     CDS             complement(3489..5216)
                     /locus_tag="PKN92_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49049.1"
                     /translation="MKRFTFYAVILLLSLGFYFQTAPGVLAEAGAVVEGEHVAEGEIV
                     PEGETPTEGEVLQEGEPVSEGEPVVEGEEPPVDGLTDVALVFPTGDIFVPAGSQAVNL
                     VLAARIQGEGTSYHIETTRVYFVLNDRYVTAEGHFQDLFYLPVTLDLEDGLPAYPLNV
                     KVIAYDEDGEHYVESAVHSPAVFAASDMDLNGFVDDPFSTFSGPGDCWIYETAHEDSA
                     TNIFMMALAPGKRITPLIFQLPPTEPETEGEDASEGETIASEKSDAPIKTQYTLLEVP
                     GQLVEPVEKGILIFASTTDAETLYAPYEVEEMLWLQPGTLAPGSRYQDLKIILSIDQG
                     STFYDLPESRTSRRPLTLIFPDCVRRNGMRHEFSGYPTVVDSDPQWGIGLKPEEGDWS
                     AEGIAVDSLSQPAGYLRAVLKRLALTGYFEISVPGFLTVIPSPETGIDFGAVPLNKPS
                     FKTIAVANHGDTAINCSVRLKDSARVFFLQSDQALRLPPGATQNFKLQFNPGEAKAYT
                     ATLVFESSDGNYQSMVIKGQGEAEAKEAGLLGCGMQSAAPLSYTADLLLLLLVAAVLI
                     TCRKRCFRS"
     gene            5503..6426
                     /gene="rbsK"
                     /locus_tag="PKN92_09410"
     CDS             5503..6426
                     /gene="rbsK"
                     /locus_tag="PKN92_09410"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNZ49050.1"
                     /translation="MAGKKIAIAGSANMDLIMRVAHFPKPGETLAGKDFTTAHGGKGA
                     NQAVAAARLGAQVSFIGSVGNDSFGNQQRKEMENEGINLRYLKTDPALPTGTAILLVA
                     DSGENMIILDAGANQALHPEDIHLLKPLFSDVDRLICQLETPLDTVREALELARTYNV
                     FSILDVAPACPLPPDFIAAADLVTPNESEAEILTGIKVDSLDSARAAAERIAGWGARH
                     VVIKLGAKGSYYFGDEEIYAPSFEINAVDTTGAGDAFTAALAVVWGTLPTAEALRFAN
                     AAGALAATRPGAQPSMPSLEEVLALAGSLSV"
     gene            6508..6948
                     /locus_tag="PKN92_09415"
     CDS             6508..6948
                     /locus_tag="PKN92_09415"
                     /inference="COORDINATES: protein motif:HMM:NF016834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lecithin retinol acyltransferase family protein"
                     /protein_id="HNZ49051.1"
                     /translation="MARGDHIAVKRHGGLYKHHGIDLGDGHVIHFSGEPLNSKTPIVE
                     KVAMEEFLKDGELEVVRYSSNKKPFSAEETIQRAEKRLGEGRYNLLRNNCEHFASWCK
                     NGKARSTQVTKLLWAAGSIAVVGVVALILYQATSESENNTGREG"
     gene            7014..7937
                     /locus_tag="PKN92_09420"
     CDS             7014..7937
                     /locus_tag="PKN92_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HNZ49052.1"
                     /translation="MSKSFSTTKYTLGVVQMAMEEKVENNILHALDMCEQAVSQGAQV
                     LLLPELFSSLYFCQREDVERFRYAEPADGDLKRRFAAFAEKHKTAVIVSFFEKRAPGL
                     YHNSAQVLDADGSTLGIYRKMHIPDDPAYYEKFYFTPGDLGFKNFKTQFGSIAPLICW
                     DQWYPEAARLAALQGATALLYPTAIGWHPYEKETYGKQQLDAWITVQRGHAIANGTYV
                     AAANRLGYEAEEGQAGILFWGASFICDPQGVMLAQGSHDKEEILLAEIDTEHLEMIRC
                     NWPFYRDRRIDAFSGLQKRFIDETTAEGMHS"
     gene            7934..8977
                     /locus_tag="PKN92_09425"
     CDS             7934..8977
                     /locus_tag="PKN92_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNZ49053.1"
                     /translation="MTPVHRFPAEWEPHRATHLAFPSNCADWPGKFAPAQWAFVEMVR
                     HLAEEETIRLAVTTEKRQQQATRMLQDAHVPMDAIEFHLCPLDRGWMRDISPFFVFDA
                     QNDRKAVLFRFNGWAKYDNYHLDAQWGQEISSALCLKSLKAVHKKRHIVLEGGAVDTN
                     GRGDLLTTGECLLDPTTQTRNPGFTQEDYENAFSRYLGIQNTIWLGSGIIGDDTHGHV
                     DDICRFVNPTTVVLCEEKDSRNPNHAVLEENRERLENIRLASGKKLETVRLPMPAPLY
                     FQSLPLPASYANFYIANGRVIVPTFNDVNDRKALGILADLFPDRKIIGIHAVDLVWGF
                     GTLHCLSHEEPAE"
     gene            9029..11167
                     /locus_tag="PKN92_09430"
     CDS             9029..11167
                     /locus_tag="PKN92_09430"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HNZ49054.1"
                     /translation="MKSINLIFGCHAHQPVGNFDFVFERAYQDSYKPFLDVLEAFPAV
                     RTTLHFTGPLFDWFEKHSPAFLDRLAALVNRGQVEIMGGGYYEPLLCAIPQRDARAQI
                     QRMQRYCEKRFGKSPRGMWLTERVWEPHMAKILNESGVEYTALDDAHFLCSGLAPEAL
                     FGYYLTEDEGRAVKVFPIMEKLRYLVPFHQVHETIDWLREHATESGERCAVLHDDYEK
                     FGVWPGTNKSVYGEGWLERFFQALTDNKDWLRCVTYSDYLDRVSAQGRTYITCASYDE
                     MMTWALPTAMQRNLVEARKNLSRHHDLNQQCAPFMRGGFWRNFLAKYPESNNIQKRML
                     RASARLENMRSPFAGEPGLEEAEKLVHQGQCNCAYWHGVFGGLYLNHLRTALYEKIIA
                     ADAVMDALDGSVKSVTKVEITDFDGDGNPEAILENDALALFFSPTDGGTLFEIDYKKQ
                     AFNFGNVLTRREEVYHDALKQGTASVAREAGGDVSIHDIVNAKEADLHYYLIYDPWRR
                     VSLRDRFLESSATAETLWSGTEPELGSHASSAYDLQTDLNSITLSKHGQLFNASEDVL
                     ISKKITLPPGGASFQISYTLSTETSFPANTLFGMEFCLNLLTGDAHDRYYWSEEQDLR
                     SMRLGARGKIDALSHLALRDEWQGIQINLRFSLPARVFHFPIETVSQSEGGQERVHQG
                     CVVLPGWTLADTETGSFSVQITVDIEALSS"
     gene            complement(11162..>11380)
                     /locus_tag="PKN92_09435"
     CDS             complement(11162..>11380)
                     /locus_tag="PKN92_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49055.1"
                     /translation="PEGGRNGRNGEMTEACTGTRMECVPGHISATNSSTCLRLNLLGF
                     QYGKMRFDIEFAEFPDAASDNLENLQKS"
BASE COUNT         2940 a         2917 c         2761 g         2762 t
ORIGIN      
        1 tccgtatttt tctcggagag cagcgttgcc tgcatgtttt taggtgtttc aggcactgta
       61 cctaaaaacc aatgatgcat cacctgcagg agccgcgccc gttctttacg ccacgcttca
      121 acggtcgtca ccgcttctat ttttccgtca acaatcggca aaagcggatc cggaagagat
      181 gcctgactcg gcatgtcatc aaaaaccgga tactgttctc cgctctctgt aagccactgg
      241 ctgaatggtt cacttgcagg aatggtatcc aaaaaagctt ccagagaggg tggagttgca
      301 gaggcggacg ctacaagcat aacaacagtt aaaatgacca tgaggctgtc gctccttcac
      361 ttgttttctt tatgggaaag gtaccgactt ctggcgacct gtatcagatt ttattttttc
      421 ttttttgatt ttaccggacc tgttccgggc tgaaccatct cctgtttggg atactctttt
      481 tctcccagcc gcaaagagcg cacccaaaag aaaacgcctg ttaaaagaat aaagatgctg
      541 taaaaagttc cccggctgac cccgaaaaag agagaggcgt caggttccct gaagtgctca
      601 cagaagattc ggaaaattgc ataaaagaac agaaactccc cgcacagtct cccgggaaaa
      661 cgccatgctg tggtcttcca gaaacgccat tgaaggatca ggaaaaggac aagaccttcc
      721 agaattgctt cgtacaactg cgaggggtga cggggctgaa taaattcaag tgccaccccg
      781 ggatcacttc tgggaaaaac aactgcccag ggcactgtcg taatctttcc ccataattcc
      841 ccgttgatga agttggctat ccgccccaaa aaaagcccca ggggcgcgat aggcgtgata
      901 agatccccaa tctggaaaaa ggacactttc gtttttcgtg caacccaaaa gcaagcgatg
      961 ataacgccaa gcagtccgcc gtgaaaagac atgccgccat gccatacttc aaacaaaaaa
     1021 aacggtctgg caatcaattc aggcagcgca taaagcacac aatatccgac tcgtgccccg
     1081 acaaggacgc cgagcactaa tgcgtacaaa agcacctctt cctcttcccg tccgaaagga
     1141 gatcgccggc ggcttatcat aagacggtga atgcagaccc ccagaacgaa ggcaagcaca
     1201 taagacagtc cgtaataact gactcccata tttccccata aatgaatcag cttgggatcc
     1261 agatagttca cccagtaacc tgtatttgta gtcatcatct ctccgttatc caatcagcgt
     1321 gcaatagact gttataaaaa ccggcaaagt gccggagcta tcgttcgaac aaatccttat
     1381 caaactttac aatacaacac actttatttg ccataagtgt tcaaaaataa tatacatttc
     1441 caaatccggt acgggacgtt ctgcaggcat catacagcgg aaaaatgcaa taaaagttga
     1501 ttttttacct ttagactgat actattcgca tcattgcgcc caatgcaaac gacctgaact
     1561 tttcccacat ccgaacactt caagcgtatc attcgggaaa aataacgatc cgatggagaa
     1621 taacatgcat ccctaccgta cacataattg tggtgaactg cgccagaaag actccggaaa
     1681 tactgtaaaa ctgtccggat gggttcaccg gaagagagac cacggcggcg ttgtctttat
     1741 cgacttgcgc gaccattacg ggctcactca aattgttatt aatccggata cccctttctt
     1801 ttccgatgca gaaaagattc gcaatgaaag cgtcatcacc gttgtgggcg aggtggtaaa
     1861 gcgcacagat gaaacagtca acgccaatat ggcaacagga gaaatcgaag tcgtcgctgc
     1921 agatatgacc gtagaatctg ccgttgacga aacgcttcct ttcacagtga atcaggaact
     1981 ggactgccct gaagaaaccc ggcttaccta ccgattcctt gacttgcgtc gggaaagaat
     2041 gcatcagaac atactgcttc gttcaaaagc gactgctctc gtgcgtgagc atctggtagc
     2101 caaaggcttt gtggaatacc atacgcccat ccttacaagc tcttcccccg aaggtgcccg
     2161 ggactatctg gtacccagcc gcctttaccc cggtcagttt tatgcgcttc cgcaggctcc
     2221 ccagcagttc aaacagctgc tgatggtatc gggatttgac cgttactttc agattgcgcc
     2281 ctgctttcgc gatgaggaca gccgcgccga ccggagtccc ggtgagttct atcagattga
     2341 tatggaaatg tcttttatca cccaggacga cctgtttgca gagctcgaag aactcatggt
     2401 tcgccttttt aaagaacttt ccaccaaaga aattgcggcg gaaacattcc cccggatcgc
     2461 ctaccgtgac gcaataaact ggtatggtac tgacaagccg gatatccgtt atgacatgaa
     2521 aatggtggac tgtacagata tttttcaaaa ctgcgaactg aaagtattca gccatcaggc
     2581 agcagcaggc ggcgtcatca aagtactcaa tgccaaagat gccgcacagc ggccccgtaa
     2641 gttttttgat gacatggagc gctatgcccg cggtgagggc gccaaaggtc ttgcctggct
     2701 ggcactgaaa gaaggcgaag taaaagggcc catcgccaaa ttcatcagtg aagacgagat
     2761 gaagcagtta tttgagcgca cagatgctgt tgaaggtgat gtactctttt ttggtgccgg
     2821 caatgcagac gaaacacgcg agctgatggg taaggttcga cagatgcttg ccagacaact
     2881 ggagctggtc gatgccagga aagtggcttt ctgctggatc gtcgactttc ccatgtttga
     2941 aagaaatccc gataccggtc agattgaatt cagccacaat cctttttcca tgccgcaagg
     3001 cggtatggat tcgctgctga ataaagatcc gctggacatt cttgcctatc agtatgacat
     3061 cgtctgcaac ggcatcgaac tctcctccgg tgccatccga aaccatcggc ccgatatcat
     3121 gctgaaagca tttgaaatcg ccggctatcc ggagagcgaa gtcaagacta aatttccggc
     3181 actgtggaaa gcgttccatt ttggagcgcc cccgcacggc ggcatcgccc ctggtcttga
     3241 ccgcatcgta atgcttctcg ccgatgagcc aaatatcaga gaaatcatcg catttcctct
     3301 gaaccagaag gctcaggatc ttctaatggg tgccccgaac tttgtcacgc aggctcagct
     3361 cgatgagctc agccttgaga ttaagctccc tgctgataca gacgcttcct gacctccgat
     3421 cagacacata aaacgcacct tcacggccaa ggctgtcgca gaccacatcg gctctctgaa
     3481 aggagatatt aggagcggaa gcaacgtttg cgacacgtta tcagaacagc tgccacaagt
     3541 agaagcagca gtagatctgc tgtgtaagac agcggcgcag ccgattgcat tccgcagccg
     3601 agaagtcctg cttcctttgc ttccgcctct ccctgccctt ttatgaccat gctttgatag
     3661 ttaccatcgg agctttcaaa aacaagggtg gctgtgtagg cctttgcttc acccggatta
     3721 aattgcagtt tgaagttttg cgttgccccg ggcggcaaac gcagtgcctg atcgctctgc
     3781 aggaaaaaga cgcgggcgga atcctttagc cggacactgc aattgatagc agtatctcca
     3841 tgattggcga cggcaatggt tttaaaagaa ggtttattga gaggcacagc tccaaaatct
     3901 atgcccgtct caggagaagg aataacggtc agaaagccgg ggacagaaat ttcaaagtag
     3961 cctgtcagcg ccaggcgttt caaaacggcc cgaaggtaac cggcaggctg ggaaagagaa
     4021 tctacagcaa tcccctccgc actccaatcc ccttcttccg gtttgagccc tatgccccat
     4081 tgcgggtcac tgtcaacgac cgtaggatag ccggaaaatt catggcgcat tccattgcgc
     4141 cgcacgcaat ccggaaatat gagagtcaga ggtcttctgg aggtgcggga ttcgggcagg
     4201 tcgtaaaagg tacttccctg atctatggag agaatgatct ttagatcctg atatctgctt
     4261 ccgggagcga gggtgcccgg ctggagccac agcatttcct ccacttcgta gggcgcatat
     4321 aaagtttcag catcggtcgt tgatgcaaaa atcagaattc ctttttccac aggctcaacc
     4381 agctggccgg gcacttcaag aagtgtgtat tgtgttttta ttggagcatc tgatttttca
     4441 gaagcgattg tttctccttc cgacgcatct tctccctccg tttcaggctc tgttggcggc
     4501 aactgaaaaa tcaaaggcgt tattcttttc cccggtgcca atgccatcat gaaaatattg
     4561 gttgcgctgt cttcgtgagc tgtttcgtat atccagcagt caccaggtcc ggaaaaggtc
     4621 gaaaagggat cgtcaacaaa accattcaaa tccatgtctg atgctgcaaa caccgctggg
     4681 ctgtgaacag cgctttcaac atagtgctca ccatcctcat cataggcgat cactttaaca
     4741 ttcagcgggt atgcaggcag accatcttca agatcaaggg taacaggaag ataaaacaag
     4801 tcctggaagt gcccctccgc tgtcacatat cggtcattta aaacaaaata tacccgcgtt
     4861 gtttcaatat gataggaagt gccttcccct tgaatacgtg ctgccagcac cagattcacc
     4921 gcctggcttc cggccggaac aaaaatatct ccggtcggga aaaccaacgc cacatcggtg
     4981 agcccgtcaa caggaggttc ttccccttcg accacgggtt ctccctcgga gacaggttcg
     5041 ccttcctgca gtacttctcc ttccgtgggg gtctctccct ccggaactat ttcgccttca
     5101 gcgacatgct ccccttccac aacagcacca gcctcagcca gcactccagg cgcagtctga
     5161 aaataaaatc ccagacttaa aagcaggatt accgcataaa aagtgaatct tttcattgaa
     5221 aactctttct gtgtactcta ctgatatccg aagtcatgtc tcggaacagc ccggaaaaca
     5281 gatatccgcc ggcaaatgta tttacgcact aatgctataa actatacact ttgtcacagt
     5341 gaaacggcaa acattacgcc ctgtcttcgg ggcttcttcg gatagttttc gattaaatat
     5401 tcatgcttcg gtcaggtatt ctggtattct atagtgactg caacaatccc gggcaactca
     5461 taacgcatcc gggtactact gagaaaaata gagaacaaca atatggcagg caaaaaaatc
     5521 gcaattgcgg gcagtgccaa tatggatctg atcatgcggg tcgcacattt tcctaagccc
     5581 ggggaaacgc tggcaggcaa agatttcaca accgcccatg gaggaaaagg cgccaatcag
     5641 gcggttgctg cggcacgatt gggtgcgcaa gtctctttca tcgggtcggt cggcaatgac
     5701 tcttttggga atcagcagcg gaaagaaatg gaaaatgaag ggatcaatct gcgttattta
     5761 aaaacagatc cggctttgcc taccggaact gccatccttc tggtcgcaga ttccggcgaa
     5821 aacatgatta ttctggatgc aggcgccaat caggcacttc acccggaaga tattcatctg
     5881 ctgaaaccgc tcttttccga tgtggatcgg ctgatctgcc agctggaaac tcctctggat
     5941 accgtgaggg aagcgctgga acttgcccgg acatacaatg ttttctccat actggatgtt
     6001 gcgcccgcct gcccgctgcc tccggatttt attgctgctg ccgacctggt cacgccgaat
     6061 gaatcagaag cagaaattct gacaggcatc aaagtcgact ccctggactc tgcgagagcg
     6121 gcagctgaac gcatagcagg ctggggcgcc cggcacgttg tcatcaaact tggcgccaaa
     6181 ggatcttatt atttcggaga cgaggaaatt tacgcaccct cttttgaaat aaatgcagta
     6241 gacacaaccg gtgccggcga tgcatttaca gcggcgcttg ccgtcgtctg gggtaccttg
     6301 ccgacagctg aagcactccg ttttgccaat gccgctggcg ctcttgcggc aacacggccg
     6361 ggcgctcagc cttccatgcc ttccctggaa gaggtgcttg cactggccgg cagcctctct
     6421 gtttagagat tcatacgctg ccggactttc cgtgctatac tatcttcaaa aatttttata
     6481 cagcgcttta atcaggagaa ataaactatg gcacgaggcg atcatatcgc tgtaaaacgc
     6541 catggcggat tgtataaaca ccatgggata gatttgggcg acgggcatgt tattcatttt
     6601 tcaggtgaac ctttaaatag caaaacgcct attgttgaaa aagtggcaat ggaagaattt
     6661 ttaaaagacg gtgagctgga ggtggtcaga tattccagca ataaaaagcc cttttctgct
     6721 gaagaaacca ttcagcgggc agaaaaacgt ctcggagagg gccggtataa tcttttacgc
     6781 aataattgcg agcactttgc gagctggtgc aaaaacggga aagcgcgaag tactcaggta
     6841 acaaaacttc tctgggccgc aggctcgatt gcagtcgtag gcgtggttgc cctcatcctg
     6901 tatcaggcga catcggagtc tgaaaacaat acaggcagag aaggatgatg ctcccgaaag
     6961 catgaacctt tctcccttct tttaaccata tcctcactca ggagaaaata acagtgagca
     7021 aatccttttc cactaccaaa tatactttgg gcgtagttca gatggcgatg gaagaaaagg
     7081 tggagaataa tattcttcac gccctcgata tgtgcgaaca agccgtcagt cagggggcgc
     7141 aggttcttct tctgccggag cttttttcca gcctttattt ttgtcagcgg gaagacgtgg
     7201 aacggttcag atatgcggaa ccggcagacg gcgatctgaa aagacgcttt gcggctttcg
     7261 ctgaaaagca caaaaccgct gtcattgtct ctttttttga aaaacgcgct cccggtctgt
     7321 atcacaacag tgctcaggta ttggatgcag acggaagcac tctcggcatt taccgcaaaa
     7381 tgcatattcc cgatgatcct gcctattatg aaaaatttta ttttacgccc ggggatctgg
     7441 gttttaaaaa tttcaaaaca caattcggct ccattgcgcc cctgatctgc tgggatcagt
     7501 ggtatccgga agcagcacgc ctagccgccc tgcagggtgc caccgcgctt ctctacccca
     7561 ccgctatcgg atggcatcct tatgaaaaag aaacctatgg aaagcagcag ctggacgcct
     7621 ggattacggt tcaacggggt catgctattg caaacggcac ttatgtcgcc gctgccaatc
     7681 gtctgggcta tgaagctgaa gaaggtcagg caggcattct tttctggggt gcatcattca
     7741 tctgcgatcc gcagggagtg atgcttgctc agggcagcca cgataaggaa gaaattcttc
     7801 ttgcggaaat tgataccgag catcttgaaa tgattcgctg caactggccc ttctaccggg
     7861 atcggcgcat tgatgccttc agcggactgc agaaacggtt tattgatgaa actactgcgg
     7921 aaggaatgca ttcatgacac ccgtccatcg ctttcctgcc gaatgggaac ctcatcgtgc
     7981 aacgcacctt gcttttcctt caaactgtgc cgactggccc ggcaaatttg ccccggcaca
     8041 atgggcattt gtcgaaatgg ttcgccatct tgccgaagaa gaaaccatcc ggctggctgt
     8101 aacaacagaa aaacgacaac agcaggcaac gcgcatgctc caggatgccc acgtcccgat
     8161 ggatgctatc gaattccatc tctgccctct tgacagaggt tggatgcggg acatttcacc
     8221 tttttttgtc tttgatgcgc aaaatgaccg gaaggctgtc cttttccgtt ttaacggttg
     8281 ggcaaaatac gacaattacc atctggatgc acaatggggg caggaaattt catctgctct
     8341 gtgtttgaaa agtcttaaag ctgttcacaa gaaaaggcat attgttctgg aaggcggtgc
     8401 cgtggataca aacggtcggg gcgatcttct tacgaccgga gaatgcctgc tcgacccgac
     8461 cacacagaca cgaaatccgg gttttacaca agaggattac gaaaacgcct ttagccgtta
     8521 tctgggtatt cagaatacga tctggctggg ttcaggcatc atcggtgacg acacccatgg
     8581 acatgtcgac gacatctgcc gttttgtaaa tccgacaacc gttgttctct gtgaagaaaa
     8641 agacagccgc aaccccaatc atgccgtgct cgaagaaaac cgggaacggc tggaaaacat
     8701 ccggctggcc tccgggaaaa aacttgaaac cgtacggctt cccatgcccg ccccgttata
     8761 ttttcaatcg ttaccgctgc ctgccagtta tgcgaatttt tatattgcca acgggcgtgt
     8821 cattgtgccc accttcaacg atgtaaatga ccggaaagca ctcggcattc ttgctgatct
     8881 tttccctgac agaaagatta tcggtattca cgctgttgat cttgtgtggg gtttcggcac
     8941 gcttcattgc ctctctcatg aagaacctgc ggaataatct ttcaccggat tcagttacaa
     9001 caccttttta ctgcaaatgg aaaagtatat gaaatcaatc aatcttatct tcggatgcca
     9061 cgcccatcag cctgtcggca actttgattt tgtctttgag cgggcgtatc aggattctta
     9121 taagcctttt ctggatgtcc tggaagcttt cccggcggta cggacgacac tgcattttac
     9181 cggccccctt ttcgactggt tcgaaaagca cagtcccgct tttcttgacc ggctggcggc
     9241 actggttaac cgcggtcagg tggagatcat gggcggcggt tattacgagc ctttgctttg
     9301 cgccattcca caaagagatg cccgtgctca gattcagcgc atgcagcggt attgtgaaaa
     9361 acggtttgga aaatccccgc gcggcatgtg gctgactgag cgggtctggg aaccgcatat
     9421 ggcaaagatt cttaatgaat caggcgttga atacacggct cttgatgatg cgcactttct
     9481 gtgttccggt cttgcgcctg aagcactttt cggctattac cttactgaag atgaaggccg
     9541 tgctgtcaaa gtctttccca tcatggaaaa acttcgttac ctcgttccct ttcaccaggt
     9601 gcacgagacc atcgactggc tccgggaaca tgcaacagaa tccggagagc ggtgtgccgt
     9661 tctgcatgat gattatgaaa aattcggcgt ctggcccgga acgaataaaa gtgtctacgg
     9721 ggagggatgg cttgaacgct tctttcaggc gctcacggat aataaggact ggctgcgctg
     9781 cgttacctac agcgactatc tggaccgcgt ctccgcacag ggacgcacct atatcacctg
     9841 tgcctcttat gacgaaatga tgacctgggc gctgcccact gccatgcagc gcaatcttgt
     9901 cgaggcacgc aaaaatttga gccggcacca cgatttgaat cagcaatgcg ccccctttat
     9961 gagaggcgga ttttggagga attttctggc gaaatatcct gaatccaaca atatacaaaa
    10021 gcgcatgctc cgggcaagcg cccgattgga aaacatgcgt tcaccctttg ccggagaacc
    10081 cggacttgaa gaagccgaga aattggttca tcagggacaa tgcaactgtg cctattggca
    10141 tggtgttttc ggaggcctct atctcaatca cctgcggaca gcactttatg aaaaaataat
    10201 tgctgccgat gcggtaatgg atgctctgga cggctccgta aaaagtgtta ccaaagtaga
    10261 gataaccgac tttgacggcg acggcaatcc ggaagctatt ctggagaacg atgctctggc
    10321 tctttttttc agtccgacag acggcggtac ccttttcgag attgactaca aaaaacaggc
    10381 cttcaatttc ggcaatgtgc ttacccgtcg ggaggaagtg tatcatgacg cactcaaaca
    10441 gggaacggcc agcgttgccc gtgaagcagg cggagacgtg agcattcatg acattgtcaa
    10501 tgcaaaagag gcagaccttc attattatct tatttatgat ccgtggcgaa gagtatcttt
    10561 gagagatcgt ttccttgaat cttccgcaac agcagagacg ctgtggagtg gaacagaacc
    10621 ggaattgggg tcccatgctt cttctgctta cgaccttcag acagatctta acagcataac
    10681 gctgtcgaaa catggtcaac ttttcaatgc ctcagaagat gtcctgatta gtaaaaaaat
    10741 cacgctgcct cccggcggcg cttcttttca gatcagctac acactttcga cggagacctc
    10801 ttttccggca aacacgcttt tcggtatgga gttttgcctg aacctgctga ccggcgatgc
    10861 ccatgaccgt tattactggt cggaagagca ggacttacgt tccatgcgcc tgggtgcgcg
    10921 gggaaaaatt gacgctttat cccatctggc gctgagagat gagtggcaag gcatacaaat
    10981 taatttgcgt ttttctctgc ctgcgcgagt attccatttc cccattgaaa ccgtaagtca
    11041 atcggaaggc ggtcaggagc gggttcatca gggatgtgtg gtgctgccag gctggacact
    11101 ggcagacaca gaaacaggat cgttttcggt gcaaatcact gtcgatattg aggcgctttc
    11161 ttcatgattt ttgaagattt tccaggttgt ctgaagcggc atcgggaaac tctgcgaact
    11221 caatatcgaa acgcattttt ccatattgga atccaagcag gttaagacga agacaggtac
    11281 tcgaattcgt cgcggaaata tgccccggga cacactccat cctcgtccct gtacacgcct
    11341 ctgtcatttc tccgttccgc ccgttacgtc caccctccgg
//
LOCUS       DAOPWB010000100        11158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5924_length_11158_cov_5.764478, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000100 DAOPWB010000000
VERSION     DAOPWB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5924_length_11158_cov_5.764478"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PKN92_09440"
     CDS             complement(<1..1149)
                     /locus_tag="PKN92_09440"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNZ49056.1"
                     /translation="MTMPLEVHSAYSMMEGTARPRQLVARAVEYGMRSLVLTDTDGLY
                     GFLPFYTAARDAGINAIAGARLGRCLILARNRQGYSRLCEIISAVHLGKADKYNLGDW
                     TFSFGDEDLFLISDEMELLEKLAKKGLRPLAGIRHYGTRASRCAAEKLIGQAKEKKIS
                     SVATFPVYFLDPGDYDAHRIISAIRENAALASLPPHATAPAGAWFRSPEQIERLYGLW
                     PETLHMLARVEEECSLDLSLGKPLFPTFPLPEGESAFSLLWKMVFEGLNRRYRPLTPA
                     LLDRAHYELGVIHDQGFAPYFLIVADLVRFASENGIPATGRGSAANSLVAHALGITRV
                     DPLRYNLYFERFLNRSRKDCPDIDLDLCWRGRDQVLDYVYKRYGSDHVA"
     gene            1277..2026
                     /locus_tag="PKN92_09445"
     CDS             1277..2026
                     /locus_tag="PKN92_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HNZ49057.1"
                     /translation="MHFKVIACEVLTREVCWCVARSPHTVDLEFTTKGEHNVPARLQQ
                     RLQERVFAPAPEEVVYDAVLLVYGLCGNAVRGLKAPAGCPLVIPRAHDCTTLFLGSQE
                     AYQKHFGANPSQMWASVGYSERGKSVLSDMDMRFFFPDGISVAEVIERYGEENAQYLL
                     DTLTKCPDYADIPFLHMPETHVPEIAEKIRRHVEGTGKALRTIQGDIRLIQGLLSGNW
                     PEEEYLVVPPGSRIEGVYDGCEIVRAIVEEK"
     gene            complement(2061..3806)
                     /gene="metG"
                     /locus_tag="PKN92_09450"
     CDS             complement(2061..3806)
                     /gene="metG"
                     /locus_tag="PKN92_09450"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNZ49058.1"
                     /translation="MTSSSAQFKRTLVTSALPYANGYIHLGHIAGAYLPADIYVRFKR
                     LCGERVLYIGGSDEHGVPITLSALKENISAKEVIDRYHSANKDAFARLGISYDIYGRT
                     SWPLHVDTTQQFFVRLAETGHIHTETVQLWYSPKLARFLPDRYVKGECPRCGYTDANG
                     DECEQCGAQYTAEELLSPRANVPGDDSTPVLRDSKHWFLDLPDFTDQLKAWLDSHPEW
                     RSNVRGIAYGWVQDLRARCITRDTDWGIPIPVDDADAADKRIYVWFDAPIGYVTNTRQ
                     WGLDQGLGDDAWKCWWKDRGTRMIHFIGKDNVPFHAVIFPAMLMGQEGYITADQVVGN
                     EYLNIYNRRTGKSEKGSKSKGNMISVRWALDHLSADALRYYLCAIAPESKDSDFDWDE
                     FMARYNGELCDVVGNFVHRALTMAVKNFEQAVPPQGTLDDEDLAMLDAIIQQRDAVAE
                     ALENFRFRQAMERFIDLGRKANVYFDQKQPWVTRKSDMARTASTLYVCCQVVHGLCHL
                     MAPFMPEGAGKLAGILNIELPSGGPDGGENIWNRAGTVLAEGSPLQSPSVLFPKLDPD
                     MIAELAELHEQGRAQ"
     gene            complement(3961..4884)
                     /locus_tag="PKN92_09455"
     CDS             complement(3961..4884)
                     /locus_tag="PKN92_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNZ49059.1"
                     /translation="MSHTVNRRNFMLAAGTGLSALGSLHTSAAATTPSASATSPMKLG
                     MVTYNMGKDMSCEELISLCKKTGIAGVELRTTHAHGVEISLNTGERARTKSLFRDSGI
                     EIAGIGTACEYHSSDPREVEAQIKESIAFAQLAADVGAPAIKVRPNGIPKGEEVETVL
                     ERIGKAWGNVAQAAADFGIEVRMEVHGTEATRNLGNIRKMLDYAAQPNATVCWNSNSD
                     EEDSTGSIRSNFDLVKHAIGQVHITDIGVYQYPWQELFTLLKDSGFSGYCLAEIQYNE
                     QPERFMRYYKTLFDLYTGQYRYPNPDVYDSK"
     gene            complement(4913..6448)
                     /gene="lysS"
                     /locus_tag="PKN92_09460"
     CDS             complement(4913..6448)
                     /gene="lysS"
                     /locus_tag="PKN92_09460"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNZ49060.1"
                     /translation="MTEQDLHTGDNPESAEKGGEQELRRHRLAKLDRIRQRGDEPYKY
                     VFDRTHSIGDARNLLESMEKEADENEPPAFPARLAGRIVARRVQGKSTFMDIRDESGR
                     IQVFLGEKQVGEVAYEETKDLDIGDFIGVSGSVKKTRRGEITLFAEVYEILAKSLRPS
                     AEKYHGLADVEIRYRHRFLDLVANPEVMTVFRKRIEMIEVMRSWLRSHGFLEVETPML
                     HPIPGGATARPFITHHNTYDSQFYLRVAPELYLKKLLVGGFEKVFEINRCFRNEGVDG
                     RHNPEFTTMEMYEAYQDYMGLMDETEDMLTHMMEAIVGSRSFVYQGQEIEIGRPWKRL
                     TLFDAIRQYAGIDLESTKDRDTAARLAKSIGVEITPDMGYGKIVDEVMSEKVQPNLVQ
                     PTFLYDYPWELSPLAKRKRGNHELTERFQPFIACLEVGNAFSELNDPIDQRQRFEAQV
                     ALREAGDDEAQYLDEDFLTALEVGMPPAAGLGIGIDRMAMIVTNSPSIRDVILFPQLR
                     PGV"
     gene            complement(6507..7628)
                     /gene="prfB"
                     /locus_tag="PKN92_09465"
     CDS             complement(6507..7628)
                     /gene="prfB"
                     /locus_tag="PKN92_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_121874281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNZ49061.1"
                     /translation="MYEAELEIMTDHAKRLSELTRCLNAADVRAAIAAIEEKMAAPDF
                     WNDQEEAQKQVKRLKALKTIINAPDELQREIEDAQVLISLAEEEEDASMRPEILGQIE
                     TLQQKLDRLELNSLFTDPRDDNAAIVSIHPGAGGTESCDWADMLYRMITRFCEQRDLE
                     VEVLDHQPGDEAGLKSATIKVTGPYAYGLLKSEEGVHRLVRISPFDANARRHTSFSAV
                     EVLAEVDDTIEIEIREEDIKMDVYRSSGAGGQKVNKTSSAVRLTHIPSGIIVACQIER
                     SQHRNRATALGMLKAKLYDMELKKQEAELNAQRETQQDVAWGSQIRSYVLHPYQMIKD
                     HRTSMETGNTTRVLDGDLDPFIAAYLKWNLERKSKNKTV"
     gene            complement(7653..8498)
                     /locus_tag="PKN92_09470"
     CDS             complement(7653..8498)
                     /locus_tag="PKN92_09470"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNZ49062.1"
                     /translation="MSIVPKNNLALRLFTALPLIPLLLAVMLIPALSPVFALIVVFLS
                     FMGARELKELIAARQPEITPLRILLLAPLLPLILFAEKAPGPIFFTALHVLLFFLVVE
                     YLLQKEAPLERLAYAIAGLFYIGGLPCFFIELHRIPQSGPGLITFLLVAIGLSDTFAF
                     AFGKMLGKHKLCPAISPNKTVEGSIAGLAGGALVGLMAYAIQQIWHLEGLPGWSPLLY
                     LSAGICLSSVGQIGDLAESALKRSAGVKDSGRLFPGHGGALDRCDAFLFGGPALYCLL
                     LIVGN"
     gene            complement(8495..9235)
                     /locus_tag="PKN92_09475"
     CDS             complement(8495..9235)
                     /locus_tag="PKN92_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNZ49063.1"
                     /translation="MDTAQQIAALLDKEKIPGHVAIIMDGNGRWAEGRGKSRLEGHEA
                     GARNVKVITEACQELGVRVLTLYAFSTENWRRSKQEIDSLFQLMGRYLKQYSGQLHKT
                     NVRLLHMGENDGLPAKTVNDIQKGLDLTRNNYGMDVCVGFNYGGRREIVRAARRLAEK
                     VRQGELSPEEITEDLFAEHLYLPMHNEVDLLIRTSGEYRISNFMLWQISYSEIVVTPT
                     FWPDFSKEEFFKAIQEYQTRLRRFGGRP"
     gene            complement(9359..9919)
                     /gene="frr"
                     /locus_tag="PKN92_09480"
     CDS             complement(9359..9919)
                     /gene="frr"
                     /locus_tag="PKN92_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNZ49064.1"
                     /translation="MPHTQAMKDTKDRMKKSVESFRQELGSIRTGRANAAILDNLDFE
                     VYGSKMKLNQLGTVTVPDAHTIAVDLWDKTQIPLIEKTLRASALNINPTNDGRLIRIP
                     IPPLTEERRRDLVKLASQYAEEAKVAVRNIRRQANDIIKQFQKDGVVPEDNAHRLSDD
                     VQKWTDEHIREIDDVLKAKEADIMEV"
     gene            complement(9922..10644)
                     /gene="pyrH"
                     /locus_tag="PKN92_09485"
     CDS             complement(9922..10644)
                     /gene="pyrH"
                     /locus_tag="PKN92_09485"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNZ49065.1"
                     /translation="MASPLFKRIMLKLSGQALEGPDRSGGIDFSTVGRFCDEVAEVAR
                     LGVEIGLVVGGGNIFRGAQTERSGLDRPTGDYMGMLATVINSLALQGSLEERGILTRV
                     MSAIEMPPIAERYIRRRAIRHLEKGRVVIFAAGTGNPFFTTDTAAALRANEIGAEVLM
                     KATRVDGIYSTDPEKDSGAVKYDRLSFDDVLSQQLKVMDATAISLCRENNLPVLVFNL
                     TQSGNLLKAVLGDTVGTLVKGE"
     gene            complement(10663..>11158)
                     /gene="tsf"
                     /locus_tag="PKN92_09490"
     CDS             complement(10663..>11158)
                     /gene="tsf"
                     /locus_tag="PKN92_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNZ49066.1"
                     /translation="AAEGAVASYIHMGGKIGVLVEVNSETDFVARGDAFQAFVRDICL
                     QICSASPTYISPDEIPAAALAAEKEVYVNQALATGKPEAMCEKIAEGKLKKWYSEVCL
                     LEQPFVKDDKKSIKDLIADLTSKCGEKIVIRRFVRFQLGEGIEKEKVDLAAEVAAELA
                     KVEK"
BASE COUNT         2561 a         2947 c         2919 g         2731 t
ORIGIN      
        1 ggcgacatga tcgctgccgt agcgtttgta gacataatcc agcacctgat ctctgccgcg
       61 ccagcaaaga tccagatcaa tgtcgggaca gtctttgcgg gatctgttca ggaaacgctc
      121 aaaataaaga ttgtagcgaa gcgggtctac ccgggtaatg cccagcgcat gggcgaccag
      181 gctattggca gccgaacctc tgccagtggc gggaattccg ttttcgctgg caaagcggac
      241 cagatctgca acaatcagga aataaggagc gaagccctga tcatgaatga cgcccagttc
      301 ataatgggcc cggtcaagca gcgcaggagt cagggggcgg tagcggcggt tcagaccttc
      361 aaaaaccatt ttccacagaa gagaaaaagc actttctccc tcaggaagcg gaaatgtcgg
      421 gaagagcggt ttgcccagag aaagatccag tgaacattcc tcttccaccc gggcaagcat
      481 atgcagtgtt tcaggccaga gtccgtaaag acgttctatc tgctcaggag agcggaacca
      541 ggctcctgcc ggagcagtgg catgaggagg caaagaggca agcgctgcat tttcccggat
      601 ggcgctgatg atccgatgtg catcataatc tccgggatcc agaaaataaa ccgggaaagt
      661 ggcgacggaa gaaatcttct tttcctttgc ctgcccgatc agtttttccg cggcgcatcg
      721 ggatgcccgg gtgccgtaat gccggatgcc cgccagcgga cgaagccctt tctttgccag
      781 tttttccaga agttccattt catcgctgat cagaaaaaga tcttcatctc cgaaagaaaa
      841 ggtccagtcg cccagattgt atttatccgc cttaccgagg tgaacagcac tgataatttc
      901 gcaaaggcgg ctgtatccct ggcgattgcg cgccagaata agacagcgtc ccagtcgggc
      961 cccggcaatg gcgttgatgc ctgcatcccg ggcagctgta taaaagggca ggaaaccgta
     1021 aagcccgtcc gtatcggtca gaaccaatga acgcatgcca tactccacag cccgtgcaac
     1081 cagctgccgc ggccgtgcag tcccttccat catggaataa gcgctatgta cttccagagg
     1141 cattgtcata gtttcagtat actgaaaaat atttcagtaa tcaagaaaaa aaggaaaaaa
     1201 gaatgcaata tcagtatact gacccatgta agcaggcatg gtgccgcttc aaactgcgta
     1261 tcagggagac tgacgcgtgc attttaaagt gattgcctgt gaagtgctga cccgggaagt
     1321 ttgctggtgt gttgcccgtt ccccgcatac cgtggacttg gaattcacaa cgaaagggga
     1381 acataacgtg cctgcccgtc ttcagcagcg gctgcaggaa cgggtcttcg cccccgcccc
     1441 ggaagaggtt gtctatgatg cggtgctgct ggtctatgga ctgtgtggca acgctgtccg
     1501 tggcttgaaa gcgcctgccg gctgtccgct ggttatccct cgtgcccatg actgcaccac
     1561 gcttttcctg ggttcccaag aggcatatca gaagcatttc ggagcaaatc ccagtcagat
     1621 gtgggcatct gtaggctatt cggagcgggg gaaatccgtg ctttcagata tggatatgcg
     1681 cttctttttt cctgacggca tttccgttgc cgaggtgatt gagcgatacg gagaagagaa
     1741 tgcgcagtat ctcctggaca cacttacaaa atgcccggac tacgcagaca tacctttttt
     1801 gcatatgccg gagacgcatg tgccggaaat tgctgaaaag atccggcgcc atgtggaagg
     1861 gacaggcaag gcgcttcgca cgattcaggg ggacatccgc ctgattcagg ggcttttatc
     1921 cggaaactgg ccggaagagg aatatctggt tgttccgccc ggcagccgca tagagggggt
     1981 ctatgacggc tgcgagattg tacgtgccat tgttgaggaa aagtaaggtc ttgtccgcac
     2041 ggcgaagaga tttttttcag ctattgcgcc ctgccttgtt cgtgcaactc tgccagctcc
     2101 gcaatcatgt ccggatccag tttcgggaaa agcacggaag gactttgcag cggactgcct
     2161 tccgccagta ctgtacccgc cctgttccag atattttctc cgccgtccgg tcctcccgaa
     2221 ggcagttcga tattaagaat tccggcaagt tttccggcgc cttcaggcat gaacggcgcc
     2281 atcaggtggc agagtccgtg tacgacctga cagcagacat aaagcgtgga agctgtgcgt
     2341 gccatgtcgc tttttctggt gacccagggc tgtttctggt caaaatagac attggctttt
     2401 ctgccaagat caataaagcg ttccattgcc tgcctgaagc ggaaattttc cagtgcttcc
     2461 gccacggcat cgcgctgctg aatgatggca tccagcatgg caaggtcttc atcatccaaa
     2521 gtgccctgcg gaggtacagc ctgttcaaaa ttcttgacag ccatggtaag cgcccgatga
     2581 acaaaattgc cgacgacgtc gcacagctcg ccattataac gcgccatgaa ttcgtcccag
     2641 tcgaagtctg agtctttgga ttccggagcg atagcgcaga gataataccg taaggcatca
     2701 gcagaaagat gatccagtgc ccaccgcacg ctgatcatat tcccttttga ttttgatccc
     2761 ttttcagatt tgccggtgcg ccggttgtaa atattgagat attcattgcc tacgacctga
     2821 tctgcggtta tatatccttc ctgtcccata agcatggcag gaaaaataac agcgtggaag
     2881 ggaacattgt ccttgccgat gaaatggatc atccgggtgc cccggtcttt ccaccagcat
     2941 ttccaggcgt catcacccag tccctgatca agtccccact ggcgcgtgtt cgtcacatag
     3001 ccgatcggcg catcaaacca aacatagatt cgcttgtcgg ctgcgtccgc atcgtccacc
     3061 ggaatgggga tgccccagtc ggtatcgcgg gtaatgcagc gggcacggag atcctgaacc
     3121 cagccgtaag cgataccgcg cacattggaa cgccattccg ggtggctgtc gagccacgct
     3181 ttcaattgat ctgtaaaatc aggaagatca agaaaccagt gttttgaatc tctgagcacc
     3241 ggcgtggaat cgtcaccggg cacattggcg cggggtgaca gcagttcttc cgccgtatac
     3301 tgggcgccgc actgctcgca ttcatcgccg tttgcatcgg tataaccgca gcgcggacac
     3361 tctcctttta cataacggtc gggaaggaaa cgcgccagtt tcggggaata ccacagctgt
     3421 acagtttcgg tatggatgtg ccctgtttca gccaggcgca caaaaaactg ctgcgtggta
     3481 tccacgtgca gcggccagga ggtgcgccca taaatatcat aggatatacc gaggcgggca
     3541 aaggcgtcct tgtttgctga atggtagcgg tcaatcactt cctttgcgga gatattttct
     3601 ttcagggcgc tcaaagtaat gggcacgccg tgttcatcgg atccgccaat ataaaggacc
     3661 cgttcgccgc aaagtctttt gaagcgtaca tagatgtcgg caggaaggta ggcgcctgca
     3721 atatgcccca gatgaatgta gccgttggca taggggagcg cgctggtcac cagtgtgcgc
     3781 ttgaattgtg cagaagaact tgtcatggga aaccctttta cttcacatga ataacagcga
     3841 cgttaacaat gaatgccatc cagtataact tgctttgaat ctgccggctt aacacgcgga
     3901 gcccgccgac agggaaactg tatcaaagcg tctctgttga aaaaacgtac tcaaaaaaac
     3961 ttatttactg tcgtatacgt ccggattggg atagcggtac tgtccggtat acagatcaaa
     4021 gagcgttttg taatagcgca tgaagcgctc cggctgctca ttatactgaa tttccgccag
     4081 acaatagccg gaaaatccgg aatctttcag caacgtaaag agctcctgcc agggatactg
     4141 gtacacgccg atgtcggtaa tgtgcacctg cccgatcgca tgtttaacca gatcaaaatt
     4201 ggatctgatg cttccagtgc tgtcttcttc gtcgctgttg gaattccagc agacagtcgc
     4261 atttggttga gcggcatagt ccagcatctt gcggatattt ccaagattcc gtgtcgcttc
     4321 cgtgccatgc acttccatgc gcacttctat accgaaatca gcggctgcct gtgccacgtt
     4381 cccccaggct tttccgatgc gttccagcac cgtctccacc tcctcgcctt tcggtatacc
     4441 gttgggccgc actttgatcg ccggggcacc cacatctgcc gccagctgcg caaaggcaat
     4501 ggattccttg atctgcgctt ccacttcacg aggatcgctg gaatgatatt cgcaggcggt
     4561 tcctataccg gcaatttcaa tgcccgaatc ccggaagagc gatttggtcc gggcgcgctc
     4621 cccggtattg agggaaattt caacgccgtg ggcatgggtc gtgcgaagtt ccactccggc
     4681 gatgcctgtt tttttacaaa gtgaaatgag ttcttcacaa ctcatatcct tgcccatatt
     4741 ataggtaacc atgcccagtt tcatgggaga cgttgcggag gcagaaggtg tggtcgcagc
     4801 agcacttgtg tgcagggaac ctaatgcgct cagcccggtg cctgcggcaa gcatgaaatt
     4861 gcgtcgatta acggtatggg acattgcgga aactcctcgg tctgttgaag ggttatacac
     4921 ccgggcgaag ctgagggaaa agaatcacat cgcgtatgga cggcgaattg gtcacgatca
     4981 tcgccatgcg gtctatccct attcccagtc cggcggcagg gggcatgccc acttccagtg
     5041 cagtcagaaa atcttcatcc agatactgcg cttcatcgtc tccggcttcc cgcagagcaa
     5101 cctgtgcctc gaagcgctgc cgttgatcaa taggatcgtt cagttcggaa aaggcattgc
     5161 ccacttccag acaggcgata aaaggctgga agcgttctgt cagctcatga ttgccccgtt
     5221 tcctttttgc aagcggtgag agctcccagg gataatcgta gagaaaagta ggttgtacca
     5281 gattgggctg aactttttcg gacatcactt catctacaat tttaccgtag cccatatccg
     5341 gggtaatttc gacaccaatg ctttttgcaa gcctggcggc ggtgtcccgg tcttttgtac
     5401 tttccagatc aatgcccgca tactgacgga tcgcatcaaa aagcgtaagt cttttccagg
     5461 gacgtccgat ctcgatttcc tgcccctgat agacaaagct ccggctgccc acaatggctt
     5521 ccatcatatg agtcagcatg tcttccgttt cgtccatgag tcccatatag tcctgataag
     5581 cttcgtacat ttccatggtg gtaaattcag gattgtgtcg cccgtccacg ccttcgttac
     5641 ggaagcagcg gttgatttcg aagacctttt caaagccgcc gacgagaagt ttcttcaggt
     5701 agagttccgg agcgacccgg agataaaact gggagtcata tgtattgtga tgcgtaataa
     5761 aaggccgtgc cgtggctccg ccggggatag gatggagcat aggcgtttcc acttccagaa
     5821 aaccgtggct gcgcagccag gatcgcatta cttcgatcat ttcaatacgc ttgcggaaaa
     5881 cggtcatcac ttcggggttg gcgacgagat ccagaaaacg atggcggtag cggatttcca
     5941 catccgcaag gccgtgatat ttttcggcgg agggtctcag tgatttcgcc agaatttcat
     6001 agacttcggc aaagagtgtg atttcaccgc ggcgggtttt cttcacagag ccggagacgc
     6061 caatgaagtc accaatatca agatccttgg tttcttcgta tgcgacttcg cctacctgtt
     6121 tttctccgag aaaaacctga atccgtccgc tttcatcgcg gatgtccata aaagtactct
     6181 tgccctgaac ccggcgcgca acaatacgcc cggcaagccg cgcagggaaa gcaggcggtt
     6241 cattttcatc cgcttctttt tccattgatt cgagcagatt tcgtgcgtca ccgatggaat
     6301 gcgttcggtc aaaaacatat ttatagggtt catcgccccg ttgtcggata cggtcaagtt
     6361 ttgccagacg atggcgccgc agttcctgtt cgcctccttt ttcggcagat tccggattgt
     6421 caccagtgtg cagatcttgt tctgtcattt tgttctgttc catttgagtt gattggctgc
     6481 aggggctatg ggtaatgatt gcgctcttac actgttttgt ttttgctttt acgctcaaga
     6541 ttccatttca gataagccgc aataaagggg tcaagatcac cgtccagcac acgggtggta
     6601 tttcctgttt ccatggacgt tcggtggtct ttaatcatct ggtagggatg caggacataa
     6661 ctgcgaatct ggcttcccca ggcgacatcc tgctgtgtct ctctctgggc gttcagttcc
     6721 gcttcctgct tttttaattc catatcatac agtttcgctt tcagcatgcc cagagccgtg
     6781 gcacggttcc ggtgctggga gcgttcaatc tggcaggcaa caataatgcc tgaaggaata
     6841 tgtgtcagcc ggacggcgct gctggtcttg ttgacttttt gtccgcctgc gccggaagaa
     6901 cggtacacat ccatcttgat gtcttcttcc cggatttcta tttcaatggt atcgtccacc
     6961 tcagccagaa cttccacggc agaaaaggaa gtgtgccgcc gtgcattggc atcaaagggg
     7021 gagatgcgca ccagccgatg cacaccttcc tcggatttaa gcagcccgta ggcataagga
     7081 cccgtcactt taatggtggc acttttcaat ccggcttcgt ctccgggctg atggtcgagc
     7141 acctccactt ccagatcacg ctgttcgcag aaacgggtaa tcatgcgata gagcatatcg
     7201 gcccagtcgc aggactctgt gccgccggcg ccgggatgta tactcacgat agcggcatta
     7261 tcatcgcggg gatccgtgaa aagactgttc agttccagac gatccagttt ctgttgcagc
     7321 gtttcgatct gtccgagaat ctcaggacgc atggacgcat cttcttcttc ttccgccaga
     7381 ctgatcagca cctgcgcatc ttcaatttcc cgttgcagtt catcgggcgc attgataatg
     7441 gtttttaatg ccttcagacg cttgacttgc ttttgcgctt cttcctgatc attccagaag
     7501 tccggggctg ccattttctc ttcaatcgca gcaatggctg cgcgaacatc tgccgcattg
     7561 aggcagcggg tcagttccga caggcgcttg gcatggtctg tcataatttc aagttctgct
     7621 tcgtacataa aaggaaattc cttaaaaaac agttaattgc ccactatgag cagcaggcag
     7681 tagagggcgg gtccaccgaa aagaaacgca tcgcagcgat ccagagcacc gccatgtccg
     7741 ggaaagagcc ggcccgagtc ctttacgccg gcactccgtt tcagggcaga ttccgccaaa
     7801 tcaccgatct gtccgacgga ggacagacaa atcccggcag aaagatagag caggggcgac
     7861 cagccgggca ggccttcaag atgccagatc tgttgtatgg catatgccat aagtccgaca
     7921 agtgcgccgc ccgcaagtcc tgcgatgctg ccttcaacag ttttgttggg gcttatggca
     7981 gggcagagtt tgtgttttcc cagcattttc ccgaaagcaa aggcaaaagt gtcggagagc
     8041 ccgatcgcaa cgagcaggaa agtaatcagt cccggtccgc tttgcggaat gcgatgcagt
     8101 tcgataaaaa aacaggggag gcctccaata tagaaaagcc ccgcaatggc atatgcaagc
     8161 cgctcgaggg gtgcttcttt ctgcagcaga tattcgacaa ccagaaagaa cagaagtaca
     8221 tggagcgcag taaagaagat cggccccggc gctttttctg cgaaaagaat cagcggcaga
     8281 agcggcgcca gcagcaatat gcgcaacggc gttatttcag gctgccgggc tgcaatgagt
     8341 tctttcagtt cccgggcgcc catgaaagag agaaatacaa cgatcagcgc aaagaccggg
     8401 ctcagagcag gaatcagcat gaccgccagc agaaggggaa tcagcggcaa tgctgtaaag
     8461 aggcgtaaag ccagattgtt tttagggaca atgctcaagg tcttcctccg aaccggcgca
     8521 gtcgtgtctg atattcctgt atggccttaa aaaattcctc ttttgaaaaa tcgggccaga
     8581 acgtgggcgt aactacaatt tctgaatagg agatctgcca gagcatgaaa ttgctgatgc
     8641 ggtactctcc gcttgtgcgg atcagcagat ccacttcatt atgcataggc agatacaggt
     8701 gctcggcaaa aaggtcttcg gtgatttcct ccggagaaag ttcgccttgc cgcacttttt
     8761 cagcaagccg ccgggcagca cgcactattt cgcgtcgtcc gccgtaatta aatcctacac
     8821 agacatccat gccgtaattg tttcgggtaa gatccagtcc tttttgaata tcgttcactg
     8881 ttttagcggg cagtccgtcg ttttcgccca tgtgcagcag gcggacgttt gttttgtgaa
     8941 gctgcccgga atactgcttc agatagcggc ccattagctg aaacagagaa tcgatctcct
     9001 gcttcgagcg gcgccagttt tctgtgctga atgcataaag cgtcaggaca cgcacaccga
     9061 gttcctgaca ggcctcggtg atgaccttta cgtttcttgc ccctgcttcg tgcccttcga
     9121 ggcgggactt gcccctgcct tccgcccagc gtccgttgcc gtccatgata atggcaacgt
     9181 gaccgggaat tttttcttta tccaggagcg cagcgatctg ttgtgccgta tccatgtcga
     9241 tttacttccg gtgccacaat ggcatgatgc ccttgcactg ataaaaaaaa gcgacaaccc
     9301 tggacggctt gccgctacaa aaaacaggct gatgcacggc ggtgcctctc cgccgatgtc
     9361 acacttccat aatgtccgct tctttggctt tcagcacatc atcaatttcg cggatatgct
     9421 catctgtcca tttttgaacg tcgtccgaaa ggcgatgggc gttgtcttca ggcacaacgc
     9481 cgtctttctg gaactgcttg attatgtcgt ttgcctggcg ccggatattg cggacagcaa
     9541 cttttgcctc ttctgcatac tggctggcaa gtttcaccag atcccggcgt cgctcttcgg
     9601 taagcggtgg aatgggtatg cgaattaaac ggccgtcgtt ggtgggattg atattcaacg
     9661 cagaggcgcg cagtgttttt tcaatcagcg ggatctgggt tttatcccat aaatcaacgg
     9721 caatggtgtg cgcatcgggc acggttaccg tacccagttg attgagtttc attttggagc
     9781 cgtatacttc aaaatcgaga ttgtcaagaa tggcggcatt tgcccggcct gtgcgaatac
     9841 ttcccagctc ctgcctgaag gattcaacac tttttttcat ccggtctttc gtatctttca
     9901 tggcttgcgt atgaggcatg gttactctcc tttcaccagc gtgccgacgg tgtcgccgag
     9961 cactgctttc aaaagatttc ctgactgcgt aagattgaag acaagcaccg gaagattgtt
    10021 ttcccggcac aacgaaattg cggttgcatc catgactttt aactgttgtg acagcacatc
    10081 gtcaaaagac agcctatcat attttacagc gccggaatct ttttcgggat cggtggaata
    10141 tatgccgtcg acacgggtcg ctttcatcag cacttcggca ccgatttcgt tggcgcgtaa
    10201 tgcggcagct gtgtccgtgg tgaaaaaggg gttgcctgtt ccggcagcga aaataaccac
    10261 gcgtcctttc tcaagatgcc ggatcgcacg gcggcgaata taccgctcgg cgatgggcgg
    10321 catttcaatg gcactcatca cacgggtaag aatgccccgc tcttccagtg acccctgcag
    10381 ggcaagagag ttgatgacgg tggcgagcat acccatatag tcgcctgtgg ggcggtcgag
    10441 acctgagcgc tctgtctgag caccccggaa aatattaccg ccgccgacga ccagtccgat
    10501 ctcaacgccc aaacgggcaa cttccgccac ctcatcacag aacctgccta ccgtggagaa
    10561 atcaatgccg ccgctgcggt caggaccttc aagtgcctga ccgctcagct tcagcatgat
    10621 tcgtttaaat agcggtgatg ccattgaggg agaaccctgt ggttattttt cgacttttgc
    10681 aagttcggca gccacttcag cagcaaggtc aaccttttct ttttcgatgc cttcgcccag
    10741 ttggaagcgg acgaagcgac ggatcacgat tttttctccg catttggaag tcagatcggc
    10801 gatcagatct ttgatgctct ttttgtcatc ttttacgaaa ggctgttcca gaaggcacac
    10861 ttcactgtac cactttttca acttgccttc ggcaattttt tcgcacatgg cttccggttt
    10921 tccggtagca agcgcctgat tcacataaac ctctttttcc gcggcaaggg cggcggctgg
    10981 aatctcatcc gggctgatat aggtcggtga agcggagcag atctgcaggc agatatcgcg
    11041 gacaaaagcc tgaaaagcat cgccccgtgc cacaaaatcg gtttcactgt tgacttcaac
    11101 aagcacgccg attttaccgc ccatatgaat gtaagaagcg acggcacctt cggcggcg
//
LOCUS       DAOPWB010000101        11152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_5929_length_11152_cov_5.053438, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000101 DAOPWB010000000
VERSION     DAOPWB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5929_length_11152_cov_5.053438"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1547
                     /locus_tag="PKN92_09495"
     CDS             264..1547
                     /locus_tag="PKN92_09495"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HNZ49067.1"
                     /translation="MKLAIIGGGSSYSPELLDGLFARIDQIPVSEVWLMDPDEKRLEI
                     NAAFARRMIRHRCVPCSIHVTTRLEEALKEARYAITQIRVGQMAARIADEKLGLKYGI
                     VGQETTGLGGFACALRTIPRILEIAHLMEEVAPDAFLINFTNPAGINTEAVIKHSKIR
                     TVGLCNVPIGMIMDASKHLNYDPAGVTMDYVGLNHLSWVRHFYYKGEDVTDAVLEKFI
                     EHARLEWEEEDTRNNMIEAIRRLGMFCNYYLQYYYSTDTALRTIQHKTKTRGEEVLEV
                     ENALFAKYQDESLSEKPEELSKRGGAHYSTAAFHLVEAIENNRQSRQVVCCRNNGAVP
                     TFDDDVAVEVSAIIGKDGAKAIQQQAPAPSIRGLMQLVKAYESLTVEAAVKGDRDAAY
                     EAMLLHPLMPNAGLCTKLLDELLEINAPWLQGTFF"
     gene            1577..2470
                     /locus_tag="PKN92_09500"
     CDS             1577..2470
                     /locus_tag="PKN92_09500"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNZ49068.1"
                     /translation="MSTTPVCETSLSDRGPDKSGKVRDIYDLGDRLLLVATDRISAFD
                     WINPVGIPDKGKILTQISLFWFEQMKDLCRNHLISADVADFPKEFQERASMFEGRSML
                     VRKCEMFPVEFVIRGYLAGSGLKEYREKGTVCGIALPAGLVEADCLEEPIYTPATKAT
                     DGHDINISPEEAGKIIGEDLNRKVSETALAIYKRGREIAADRGIILCDTKFEFGMLDG
                     ELVLADEILTPDSSRFWPADQYAPGKAQPSYDKQFVRDHLEAVGWDKDSPPPPLPADV
                     IERTREKYLEAYCRLTGKTGL"
     gene            2489..3628
                     /locus_tag="PKN92_09505"
     CDS             2489..3628
                     /locus_tag="PKN92_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNZ49069.1"
                     /translation="MKKTLFLLLILGLVSLPLAAQMPGLDNLCRLKNAESRSISPENF
                     TGEKGKGGMATLEEGTAAKAARELGQGWKVNPYVHIEPGTTFTLGEVKDSGVINHIWM
                     TPVGDYRCMIFRFYWDGEEKPSVEVPVGDFFAAGWGMGKEPQLTSLAVCVNPRSGFNC
                     YWQMPFRKGFRITIENLSEKPATIYYQIDYTLEKVKRDTPYFHAQFRRTNPLPEKEVF
                     TIVDGIRGRGHYVGTYVTHGANSPGWWGEGEIKFYIDGDTDFPTICGTGEEDYFCGSY
                     GYNERNEDGKEVYSSFSSPYTGFYHVPYADGKYRRFGQYRWHITDPVHFRNDLKITLQ
                     SLGWQSEGRYLPLQDDLGAVSFWYQLEPHAPFPALPTAEQLTITD"
     gene            4111..8601
                     /gene="cas12b"
                     /locus_tag="PKN92_09510"
     CDS             4111..8601
                     /gene="cas12b"
                     /locus_tag="PKN92_09510"
                     /inference="COORDINATES: protein motif:HMM:NF033949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas12b"
                     /protein_id="HNZ49070.1"
                     /translation="MADDLSTQRAYTLRLQGTDPEDQSWRDALWMTHEAVNAGGRAFG
                     DWLLTLRGGIAHELADTPVKGKKDITDELRKNRRILLALSWLSVESRRGAPDKFIVAG
                     GEEPAGSRNEKVLQALKEILKRRGLSAEESESWMSDCRASLSAAIRDDAVWVNRSAAF
                     DDAQVRIGASLTREDIWDMLDPFFGSREAYLTPAKKKKEDEDSSEGTGEEKAKDLVQK
                     AGQWLSSRFGTGKGANFTAMAEVYSKISEWAGTAQEGVSGKEGIKNLADALAAFSPVS
                     QNLEGVLKLISGPGYKSATRNLLGELDSLPVVSRDHLSALHEKAAEDTVKCKESTGTK
                     GRRPYADAILNDVEKRCGFTYLTDSDNRSVSILDTSEFPSDYKWGTARHSEFAVILDH
                     AARRISVAHSWIKLAEAERDRCEEDAAKVYDLPDKVKEWLDTFCSNRSDISGAQGEGY
                     RIRRKAIEGWKEVVASWGRSSCITAEDRVAAARALQDDPEIDKFGDIQLFEILAQDEA
                     LCVWHKDGDVAKSPDAQMLIDYVLASDAESKKRRFKVPAYRHPDALLHPIFCDFGNSR
                     WDITYDIHGARGKKKAKRGSKKEEAMPRGVAMKLWTGSDVLSVSLRWQSKKLAADLAL
                     DQEAEEVTDTAAVSRADRLGRAAAGIDRGAGVTIAGLFEEAHWNGRLQAPRQQLEAIA
                     AVRDNQKLSSEERERRIAFMKDRIRWLVTFSAKLRPQGPWHSYAPTQGLQSDPKYWPH
                     SEINKKRKGQAKLILSRLPGLRILSVDLGHRFAAACAVWETMSSEAIQEACRLANHQL
                     PAPADLYLHLKRTVQKNLIDGEKTVEESTVYRRIGADRLPDGTAHPAPWARLDRQFLI
                     KLQGEEKVREASNEEVWQVHLMESALGLSFPLIDRLVYAGWGGTEKQAARLEALREKG
                     WKPTGTPADQDEEGGGYKPSLAVDELMFSAVRTLRLALKYHGDRARIAFALTADYKPM
                     PGDTRYYFSEAKDRSSGADAAEREAKHKDYLLDMLLLWHDLAFSRKWRDEEAKELWNL
                     HIAALPGYQAPAAPIQEEAGQGRKKAREEARAKMTPAAEALLADGTLREKLHGLWKER
                     WEKDDAQWKKHLRWMKDGILPRGGRAATPSIRYVGGLSLTRLATLTEFRRKVQVGFYT
                     RLFPSGEKREIKEAFGQTALDALERLREQRVKQLASRIAEAALGAGRVSRTALKQDPK
                     RPEARVDAACHAVIIENLEHYRPEETRTRRENRGLMNWASSKVKKYLSEACQLHGLFL
                     REVPAGYTSRQDSRTGAPGMRCQDVTVKTFLNSPFWQKQCVQAQKNKSTARDRFLCAL
                     KEAVAQGGMEEEKKMGPIRVPVPGGEVFVSADAASPAAKGLQADLNAAANIGLRALLD
                     PDWPGKWWYVPCDRKTAYPAKEKVEGSAAVDVKQALPFVLPEEKENKGKTKGGKKGKG
                     EVMNLWRDVSAEPLMTGQWLDYTAYRKEVENRVIQVLTAQLKARNPLRFGNLGDEEEI
                     PY"
     gene            8615..10336
                     /gene="cas1"
                     /locus_tag="PKN92_09515"
     CDS             8615..10336
                     /gene="cas1"
                     /locus_tag="PKN92_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNZ49071.1"
                     /translation="MDESLWPARNVAEYAYCPRLFYFMQIEGVFIPSYDTEEGKSVHG
                     RVDKPSAAPQPVPSEEEEDTERPVAVRSLTLTSKALSLTATLDLAEIQGTTAVPVEYR
                     KGRPRHFFRRPAKPEEEQPEETLSQEPEPWPTDRVQVGLQAVLLEEAGYTVEKAVLYY
                     AAEKRRLTIPVDAALKEEALRTLEEAKECAGGTRPLPLINDPKCPRCSLQPICLPDEI
                     NLHRAHSDDEEWTPRRIWPPRDDGIHVVAQAEGLKAGVRGKELNVYDKNGATVKSVPL
                     ANIESLSLLGSVQLTTQALHALAGLNIPIAFLSSAGRLVSMMDPMDSVSAEIRKAQVR
                     KFDQPEVCLALAQALISSKIQNQRTLLMRNHSALPPGVADSLLREINGVLKADNIETV
                     RGHEGRAAAIYFRHFSGMIKGKPGQEFDENGRQRRPPPDPVNACLSMAYTMLAHECTA
                     AARLARLEPSIGGFHVSRPGRPALALDLMEPFRPLIADSLALSCFNRGELTEGHFSRS
                     AAGCLFTDAGRRSFFNALGRRMDTEVTHPCYGYRLGYRRMIVIHARMVAAWLMGEIDT
                     LEFLTTR"
     gene            10354..10644
                     /gene="cas2"
                     /locus_tag="PKN92_09520"
     CDS             10354..10644
                     /gene="cas2"
                     /locus_tag="PKN92_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNZ49072.1"
                     /translation="MRRCYLVCYDIRDPKRLRRIHRILRGYGEAWQYSVFFCVLKDID
                     RVRLQADIEEEINHKEDQTVILDLGPNEKEARKNTTVIGTALPEPESGFVVI"
BASE COUNT         2715 a         2856 c         3187 g         2394 t
ORIGIN      
        1 tttccgtggc gaattcgagt gcctgtcttt ttcctgaccc gcttgtgtgc caataagaaa
       61 aaatgcgtct cgatattgag ttcacggttc cgctgtctgc tatttaaaat tgactttaaa
      121 tcctgcctca agtattatga agccagcgga atcttccgca gggctggcgt gtttccgtga
      181 cgggatcttt attccggcgg ttctgcagtc gttccggcgc tatggcaccc cccgtttgtt
      241 tttaaagaga gaaagattat tccatgaaat tagcaattat tggcggcggc agctcctatt
      301 cacccgagct gctcgacggt ttatttgccc ggatcgatca gattccggtc agtgaagtat
      361 ggctgatgga tccagatgaa aagcggctcg agattaacgc tgcttttgcc cggcgcatga
      421 tccgccatcg gtgcgttccc tgttccatcc atgtgactac ccggctggaa gaggcgctga
      481 aagaggcgcg gtatgccatt actcagatta gagtcggcca gatggcggca cgtatcgccg
      541 atgaaaaact cgggctcaaa tacggtattg tcgggcagga gacaacaggg ctcggcgggt
      601 ttgcctgtgc gctgcgcacc attccgcgca tcctcgaaat cgctcatctc atggaagagg
      661 tggcgcccga tgcctttctc atcaatttca ccaatcctgc cggcatcaat acggaagcgg
      721 tgatcaagca cagcaaaatc cggacggtag gtctgtgtaa tgttcccatc ggcatgatca
      781 tggatgcgtc caaacatctg aactacgacc ctgccggagt gaccatggac tatgtcggcc
      841 tgaaccatct ctcctgggta cgccattttt attataaagg cgaggacgtg acggatgccg
      901 tcctcgaaaa atttatcgag catgcccgtc tggaatggga agaggaagat acacgcaaca
      961 acatgattga agcaatccgt cgcctgggta tgttctgcaa ctattatctg cagtactact
     1021 acagcacgga tactgccctg cgtaccattc agcataagac aaagacacgg ggtgaggaag
     1081 tgctcgaagt ggagaatgct ctcttcgcca aataccagga cgagtctctc tcggaaaagc
     1141 cggaggaact gagcaagcgt ggcggagcgc attattccac cgctgctttc catctggtgg
     1201 aagccattga aaataaccgg cagagcaggc aggtcgtctg ctgccggaac aacggcgctg
     1261 ttcccacctt tgatgatgat gttgcggtgg aagtgtccgc catcatcgga aaggacggcg
     1321 caaaagcgat tcagcagcag gcgcccgcac cgtccatccg cgggctcatg cagctggtga
     1381 aagcctatga aagccttacg gtggaagcgg cggtgaaagg cgacagagat gccgcctacg
     1441 aagccatgct gctccatccg cttatgccca atgcggggct gtgcacgaaa ctgctggacg
     1501 aactgcttga aatcaatgcg ccctggcttc agggaacctt tttctgataa ccctcactat
     1561 cgcaaggatc cgattcatga gtactacgcc tgtttgcgaa acgtctctct ccgaccgcgg
     1621 tcccgacaaa tcggggaagg tgcgtgacat ctacgacctg ggcgaccggc tgcttctggt
     1681 tgccaccgac cgtatctccg ctttcgactg gatcaacccg gtgggcattc ccgacaaggg
     1741 aaaaatcctc acccaaattt ccctcttctg gtttgagcag atgaaggatc tttgtagaaa
     1801 ccatctgatc tctgctgatg tcgccgactt cccgaaggag tttcaggaac gcgcgtctat
     1861 gttcgaagga cgcagcatgc tcgtccgcaa atgtgagatg ttcccggtgg agtttgtgat
     1921 tcgcggctac ctcgccggaa gcggactcaa ggaatatcgg gaaaaaggaa ccgtctgcgg
     1981 tattgccctc cctgccgggc tggtggaagc ggactgtctg gaagagccca tctatacgcc
     2041 tgcaaccaaa gccaccgacg ggcacgacat caacatcagc ccggaagaag caggaaaaat
     2101 cattggtgaa gacttgaacc ggaaggtatc ggaaacggcg cttgccattt ataaacgggg
     2161 aagagagatc gcggcagacc ggggcatcat cctctgcgac acaaagtttg aattcggtat
     2221 gctggacgga gagctggttc tggcagatga aattcttacg cccgactctt cacgcttctg
     2281 gcctgctgat cagtatgcgc ccggaaaagc acagcccagt tatgacaaac agtttgtccg
     2341 tgatcatctc gaagcggtcg gctgggacaa ggattcgccg ccgccgcccc tgcccgccga
     2401 tgtcattgaa cggacccggg aaaaatattt ggaagcctat tgccgtctca ccggaaaaac
     2461 aggactgtaa accgaaaagg agccacctat gaaaaagaca ttatttttac tgcttattct
     2521 cgggcttgtg tctcttcctc tggcggcgca gatgcccgga ttggacaatc tgtgccgtct
     2581 gaagaatgcg gaaagccgtt ccatcagccc ggaaaacttt accggagaaa aagggaaggg
     2641 cggtatggca accctcgagg aaggtaccgc tgccaaagcc gcacgggagc tcggccaggg
     2701 atggaaggtc aacccttatg tccatatcga gcccggcacc acctttaccc tcggtgaagt
     2761 gaaggattcg ggcgttatca accatatctg gatgacaccc gtcggcgatt accggtgtat
     2821 gattttccgc ttttactggg acggtgaaga aaagccgtcg gtggaagtac ctgtgggcga
     2881 ctttttcgcg gcaggatggg gcatgggcaa agagccgcag ctgacctcac tggctgtctg
     2941 tgtgaatccc cgcagcggtt ttaactgcta ctggcagatg cctttccgca agggattccg
     3001 cattaccatc gaaaatctca gcgagaaacc agcgaccatc tattaccaga tcgattacac
     3061 gctggaaaaa gtaaagcgtg atacgcctta tttccatgcc cagttccgcc gcaccaatcc
     3121 gctccctgaa aaggaagtct tcaccattgt agacggtatt cgcgggcggg gtcattatgt
     3181 gggcacctat gtgacccacg gcgcgaacag tcccggctgg tggggtgaag gggagatcaa
     3241 gttttatatc gacggcgaca ccgatttccc caccatttgc ggtacaggtg aagaagatta
     3301 cttctgcgga tcctacggat acaacgaacg caacgaagac ggcaaagaag tatattccag
     3361 cttcagttcg ccttatacag gcttttatca tgtgccctat gcagacggaa aatacaggcg
     3421 cttcgggcag tatcgctggc atattaccga cccggttcat ttcagaaacg atctgaagat
     3481 aaccctgcag agcctcggct ggcagagtga aggcagatac ctgccgctgc aggacgatct
     3541 gggcgctgtc agcttctggt accagctgga gcctcatgct cccttccccg cacttcccac
     3601 agcggagcag ctgaccatta cggattgatc catttctgac agacagcact gcacagcaga
     3661 tgaagcaaac gagtcttcat ctgctgtttt gtttctgacg ggaaatgggg cgtgatgcct
     3721 gcttgcgcag gaatgacggc ggaacagatt cggccgccat ttgcttttcc gtttgcttct
     3781 ggtgttacaa tcatattccg gaatgtggga tttctctatc cttccggaaa tgtaaaatac
     3841 cgaagccttt tcggtggcgg cctgtcttat aagaaggggt gctgcatgtg tgccgacagc
     3901 agacaacata gcagacggat ctccggactt atccggagga atattcgaaa tggtttcgct
     3961 gttcctgcct ttctttctta tatccttccc ctgtacagtc gccatgcctc tgttgcacac
     4021 atgtttttta ctgcccgttt ccttctgttt cacgaaaata tttttttctg aacatgtttt
     4081 cttctttcaa cccaataaag gagttttctc atggctgacg atctttccac ccaacgtgcc
     4141 tacaccctcc gccttcaggg aactgacccc gaagatcagt cctggcgcga tgcgctctgg
     4201 atgacacacg aggcggtgaa tgccggcggt cgcgcttttg gagactggct gcttacactt
     4261 cgcggaggca ttgcccatga actggcagac acaccggtca aagggaaaaa ggacataaca
     4321 gacgagctca ggaaaaatcg ccgcattctt ctggcacttt cctggttgtc tgttgagtcg
     4381 agacggggcg cacccgacaa atttattgta gccggaggcg aagaacctgc gggttccaga
     4441 aatgaaaagg tactgcaggc actcaaggaa attctgaaac gacgcgggtt aagtgcggaa
     4501 gaatccgaat cgtggatgag tgactgccgt gcctcgctga gtgccgccat ccgcgacgac
     4561 gccgtgtggg taaaccgaag tgccgccttt gatgatgcgc aagtgcgtat tggcgcctcc
     4621 ctcacaagag aagatatctg ggatatgctg gatcctttct tcggaagccg cgaagcctat
     4681 ttgacaccag cgaaaaagaa aaaggaggat gaagactctt cggagggtac gggggaagag
     4741 aaggcaaaag atcttgttca aaaggcaggt caatggctca gcagccggtt tggcacagga
     4801 aagggggcta atttcactgc catggcggaa gtgtattcaa aaatatccga atgggcggga
     4861 acggcgcagg agggtgtatc cggaaaggaa ggcatcaaaa atctcgctga tgcgcttgct
     4921 gcattttcac ctgtatcaca aaatctcgaa ggggttctca aactgatttc cggccccgga
     4981 tacaaaagcg cgacacgcaa ccttctcggc gagttggaca gcctgcctgt tgtgtcccgg
     5041 gatcacttgt ccgccctgca tgaaaaagcg gcagaggata cagttaagtg caaagagagc
     5101 acaggaacaa aaggacgtcg cccctatgct gatgcgatcc tgaatgacgt ggagaaacgg
     5161 tgcgggttca cttatctgac ggacagcgac aaccgctctg tgtccatcct tgacacatca
     5221 gaatttcctt ccgattataa atggggtact gcccgccatt cggaatttgc agtgattctt
     5281 gatcatgccg caagacggat ctccgtagcc cacagctgga tcaaacttgc cgaagcggaa
     5341 cgggatcggt gtgaggagga tgcagcgaaa gtgtacgatc ttcccgacaa ggttaaggag
     5401 tggctggata ccttctgcag caaccgctcg gatatatctg gtgcgcaagg ggaagggtac
     5461 cgtatccgcc gtaaagcaat agaagggtgg aaagaagtgg ttgcgagctg gggaaggagc
     5521 agctgtatta cagcggaaga tcgtgttgcg gcggcaagag cgttgcagga cgatcctgaa
     5581 attgacaaat tcggcgatat tcagctcttt gaaatccttg cacaggatga agcgctctgt
     5641 gtatggcaca aagacgggga tgttgcaaaa tcgcctgatg cgcaaatgct gatagattat
     5701 gtactggcat ctgatgctga atccaaaaaa cgccggttta aagtgcctgc ttacaggcat
     5761 cccgatgccc tgctgcaccc gattttctgt gacttcggaa attcgcgatg ggatattaca
     5821 tacgatatcc atggagcaag agggaaaaag aaggcgaagc gcgggtcgaa aaaggaagag
     5881 gctatgccgc ggggcgtggc aatgaaatta tggaccggat cagatgtcct ttctgtatcg
     5941 ctccgctggc agtcgaaaaa acttgccgct gatctggcat tggatcagga ggcagaagaa
     6001 gtgacagaca cagcggcagt ttcccgcgct gaccggcttg gcagggctgc tgccgggatt
     6061 gaccgcgggg caggggtgac cattgccggg ctttttgagg aagctcattg gaatggccgc
     6121 ctccaggcgc cgagacagca actggaggcg atcgctgccg tacgggacaa ccagaaactg
     6181 tcttctgaag aacgtgaacg gcgcattgct tttatgaagg atcggatccg gtggctggta
     6241 accttttccg ccaagcttcg tcctcagggg ccctggcata gttacgcacc gacgcaagga
     6301 ctccagagtg atcctaaata ctggccacac tctgaaatca acaaaaaacg aaaagggcag
     6361 gcaaagctga ttctttcccg tctgcccggt ctgcgtattc tttccgttga tctggggcat
     6421 cgttttgccg cagcctgtgc tgtatgggag accatgagct ctgaagcgat tcaggaagca
     6481 tgccgccttg caaatcatca gctccctgcg ccggcagatt tgtatctgca tctgaaacgc
     6541 acggtacaga aaaatctgat tgatggcgaa aagactgttg aagaaagcac agtttaccgc
     6601 cgtatcggtg cggaccgcct gcccgacgga acggcacatc cggcgccctg ggcacggctg
     6661 gatcgtcagt tcctgatcaa gcttcagggc gaggaaaaag tacgtgaggc atccaatgag
     6721 gaagtctggc aggtccatct tatggaaagt gcgctgggcc tttccttccc cctcatcgac
     6781 aggctggtct atgccggatg gggtggtacg gaaaaacagg ctgcccggct ggaagcgctg
     6841 cgtgaaaaag gctggaaacc gacaggaacc ccggcagacc aggacgagga aggaggcggg
     6901 tacaagccgt ctctggcggt ggatgaactg atgttttctg ccgtgcgcac cctgcgcctt
     6961 gccctgaaat accatggtga ccgggcacgg atcgcctttg cactgactgc tgactataag
     7021 cccatgcccg gcgatacccg ctattatttt tccgaggcga aggaccgttc atcgggtgct
     7081 gatgcagcgg agcgggaggc aaaacacaag gactatcttc tggacatgct tcttctctgg
     7141 catgacctgg cgttttcccg caagtggcgg gatgaagagg cgaaagaact ttggaacctc
     7201 catatagcgg cactgcccgg ttatcaggcg cctgctgctc cgattcagga agaggcggga
     7261 caggggcgta aaaaagcacg ggaagaggcg cgagcgaaaa tgacgcctgc tgccgaagct
     7321 ttgttggcgg acggaacgct ccgtgaaaaa ttgcacggtc tctggaaaga acgctgggaa
     7381 aaagatgatg cgcaatggaa aaagcacctg cgctggatga aagacgggat tctgccacgg
     7441 ggcggtcgtg ccgctacgcc ttccatacgt tatgtgggtg gtctgtccct gacccgtctc
     7501 gccacattga ccgaattccg ccgtaaggta caggtgggat tttatacgcg cctgttcccc
     7561 tcgggagaga aaagggaaat caaagaggct ttcgggcaga ctgcgctgga tgcccttgag
     7621 cggcttcggg agcagcgggt caagcagctt gccagtcgta ttgcggaagc agccctcggc
     7681 gcaggcagag tcagccgcac agcactgaaa caggatccga aacggccgga agcccgggtg
     7741 gatgctgcat gtcacgccgt cattattgaa aacctcgaac actatcgccc ggaagaaaca
     7801 cggacacggc gggaaaatcg cgggctgatg aactgggctt cttccaaagt gaagaaatat
     7861 ctcagcgagg catgccaact gcacggcctg ttcctcaggg aagtgccggc aggttacacc
     7921 tcaagacagg actcgcgtac cggtgctccg ggcatgcgct gtcaggacgt gactgtaaag
     7981 acttttctga attcgccgtt ctggcagaaa caatgcgttc aggcgcaaaa aaacaaaagc
     8041 acagcccggg atcgtttcct gtgtgctttg aaagaagcag tggcgcaggg tggcatggag
     8101 gaagagaaaa aaatggggcc catccgtgtg ccggtgccag gcggcgaagt ctttgtttcg
     8161 gcagatgcag cctctccggc ggcaaagggg cttcaggcag acctgaatgc cgccgccaat
     8221 attggactgc gggctttgct cgatcccgac tggcccggga aatggtggta tgttccctgc
     8281 gatagaaaaa cggcatatcc ggcgaaagaa aaagtggaag gaagtgctgc tgtggatgtg
     8341 aagcaggcgc ttccttttgt tcttcctgaa gaaaaggaaa ataaaggcaa gacgaaaggc
     8401 ggaaaaaaag gaaagggaga ggtgatgaat ctctggcgtg atgtttccgc agagccgctg
     8461 atgaccgggc aatggctgga ttatactgct taccggaagg aagtagagaa ccgtgtgatt
     8521 caggtgctca cggcgcagct gaaagcccgg aacccgctgc gcttcggcaa tctcggggac
     8581 gaggaagaaa tcccttactg aaaaggaaaa aagtatggac gaaagcctgt ggcccgcaag
     8641 gaacgtggca gagtatgcct attgtccgcg gctcttctat ttcatgcaga tcgaaggagt
     8701 ttttatcccc agctatgata cggaggaggg caagtccgtt catggcaggg tggataagcc
     8761 cagtgccgca ccgcaacctg tcccttcaga agaagaggag gataccgaac gtcctgttgc
     8821 cgtgagaagc ctgacgctga ccagcaaagc gctgtcattg acagctacgc tggatctggc
     8881 ggaaatacag ggtacgacag ctgtgcctgt ggagtacagg aaagggcgcc cccgtcattt
     8941 ctttcgccgc cctgcgaagc cggaagagga gcagccggaa gagaccctgt ctcaggagcc
     9001 ggagccctgg cctaccgata gggtgcaggt gggactgcag gctgttcttc tggaggaggc
     9061 ggggtacaca gtggaaaaag ctgtgctgta ttacgccgca gaaaaaagaa ggcttaccat
     9121 ccccgtggat gccgccctga aagaagaagc actccgtacg ctggaggagg cgaaagaatg
     9181 cgccggaggg actcggcccc tgccgctgat caatgatccg aaatgtcccc gatgctcgct
     9241 gcagcccatt tgcctgcctg acgaaatcaa tctgcatcgt gcccactctg atgatgaaga
     9301 atggacacca cggcgcatct ggccgccccg tgacgatggt atccacgtcg tagcgcaggc
     9361 agaagggctc aaagccggtg tgcgcgggaa agaattgaac gtttatgaca aaaacggcgc
     9421 aacagtaaaa tcagtccctc ttgcgaacat tgaaagcctc tcgctgctgg gctctgtaca
     9481 gcttactacg caggcgcttc atgcgctggc ggggctgaac atacccattg cctttctttc
     9541 ctctgcaggc aggctggttt ccatgatgga ccccatggat tcggtcagcg cggaaatacg
     9601 gaaagcgcag gtgagaaagt ttgatcagcc ggaagtctgt ctggcactgg cacaggctct
     9661 gatctccagc aaaattcaaa accagcgcac gctgctgatg cgtaaccata gtgcgctccc
     9721 gccgggcgtg gcggacagtc ttctgaggga gatcaacggt gtgctgaaag cggacaacat
     9781 tgaaaccgtc cgcggtcacg aggggcgggc cgccgcgatt tatttccgcc atttttccgg
     9841 aatgatcaaa gggaagccgg ggcaggaatt cgatgaaaat ggacgtcagc gcaggccgcc
     9901 gccggatccc gtcaatgcct gcctttccat ggcgtatacc atgctggcgc atgagtgcac
     9961 cgctgcagca cgactcgcaa ggcttgagcc gtctatcggc gggttccatg tgtcccggcc
    10021 gggaagaccg gcgctggcac tggatctcat ggaaccgttc agaccgctca ttgccgattc
    10081 gcttgcgctc agctgcttta accgtgggga actgacggaa ggtcatttta gccgcagtgc
    10141 cgccggatgc ctcttcactg atgccgggcg gcgcagtttt tttaacgccc tcggcagaag
    10201 aatggatacg gaagtcacgc atccctgcta cggataccgg ctcggataca ggcggatgat
    10261 tgtgattcat gcacggatgg tcgccgcctg gctcatgggt gaaattgaca cactggaatt
    10321 tttaactaca cgataaagaa aacggaggac cttatgcgca ggtgctatct tgtctgctac
    10381 gatatccgtg atccgaaaag actccggcgc atacaccgga ttctccgggg atacggcgag
    10441 gcgtggcagt actccgtctt tttttgtgtg ctcaaagata ttgaccgtgt gcgcctgcag
    10501 gcggatatcg aagaggaaat taatcataaa gaagaccaga cggtcattct tgatctggga
    10561 cccaatgaaa aagaagcaag gaaaaatacc accgttatcg gaacggccct tccagaacct
    10621 gaaagtggct tcgtcgtgat ttgactttcc aaaaataaaa ctgtatactc ataaatgctt
    10681 ctacaggagg cgaaaagact gcggaacgtg tcttcccctt caatgggcgt ggcaccgcag
    10741 cgttgttcag tttagttcca gaaaaggacg gttcacctcc gccggtgcgt cgcgagtgcg
    10801 atggtgctga ggggggggcc ggggggatac tcgttcttct tgatatcccg gcacttagag
    10861 agaatttaat ctctaaattt ggatcaaatg acagtgaaaa aagacatcgg tcaggtcgca
    10921 ctcgcaaaag cctctttata cccaataaac acaggcctca tatgagacgg cggtcgctgt
    10981 ccttggaacc aacctgatca acggacactt agatttcttg atgacccatt cattcgtgaa
    11041 atggtcgctg tccttggaac caacctgatc aacggacacc ccttgatcca cggtgattgg
    11101 ttggaccaat ataagtcgct gtccttggaa ccaacctgat caacggacac cg
//
LOCUS       DAOPWB010000102        11007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6007_length_11007_cov_5.614500, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000102 DAOPWB010000000
VERSION     DAOPWB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6007_length_11007_cov_5.614500"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2179)
                     /locus_tag="PKN92_09525"
     CDS             complement(<1..2179)
                     /locus_tag="PKN92_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49073.1"
                     /translation="MSLNSFRQKTIIAILVLLTLAVATTVTFQFILFNDLDKLGGMYD
                     IYRYYGPLTFFVDSCIHNGEFPAWNPLTYCGLPNVGNPQSFIFYPPNLIRSLLTSNPT
                     PARTNISLAIMMGLHFLFMGVCTYLLGRAHKMSVTAALTGALSFLFSALMVRRMCEYH
                     FITTMAWLPLLLLLIKKIIDAREFALKVFLSLLAGTVLGLSILGGFLQIINLMGLVSA
                     LYGLFYFLLNHDWQRQEEGGRLAFFRPFLWNGIAMAIIFLVGSGLAAVSLIPSWELGS
                     YTLRTGGQATPKYSDLWKWTPLDFYKKLVLYGGVKYEAETLRNSGIVALLLSAAGLTW
                     KKRRDSFLFLGLFLVLFECSFGPPLPLGALLEKVTPFSMSAYTRAYDFALLPLSLLAG
                     FGLDAIRAPLADRGKSLARAVGLLLIAVVLIAPITSWLPEIQYITVNRIVIVLPLVAL
                     ILMLLAGTVPLNDRARPVVVLLLMGLLFAETFAWNQSFIPYMAKAKVRDYIQDAPEPC
                     AVPIDNRRRTDPVCNRLLYRLQFSMNGVDPLHLSAVRDLLSGPPRDKGPYRGVQDWEV
                     TRENLRGNLLFKRSFWLAKQYCVGTLPGKREFYPAASTVFLEEPLDAPIPQVERKSLR
                     NSGVSEKYLEQEITQPDALFSAVPPGATKSFTVRLSPAKSAEGRPVGSCGVLHSSLML
                     WYRSNPGVVIDTELTDTQTHFSEQGIRHTTRSAAVRETGTEVPL"
     gene            complement(2262..3137)
                     /locus_tag="PKN92_09530"
     CDS             complement(2262..3137)
                     /locus_tag="PKN92_09530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ49074.1"
                     /translation="MDDFEQYVSEGDKAERISCICCGHFPHTAVYEGLERCDKCGHIR
                     AVLDWAPEKFHELYTRGYFKGGEYEDYEEEGLALRYNFMKRLADLAKRHPEGGDLWEI
                     GCAYGFFLEAAQTHFNAAGCDIAADAVESGVAARGVKAVYGDYLKMEKGGPFDVICLW
                     DTVEHMARPDLYLEKAFSELIPGGTLALSTGDIGSRWARVRGRHWRLIHPPTHVHYFT
                     QHSMGQLLARLGFEEVRFSYPPFWRNVNTTLVKLAQKHRVFSLIRPLVARTPLGRINY
                     PLNLFDLMTVYARKK"
     gene            complement(3167..4117)
                     /locus_tag="PKN92_09535"
     CDS             complement(3167..4117)
                     /locus_tag="PKN92_09535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ49075.1"
                     /translation="MSMTPKFSFVLPAYNEEDSVVAMTERLLAVAEQIEGTVEIVWIN
                     DGSSDATGMRLDSLTQRYPAVRVFHFSRNFGHMAALCAGLECARASHAVITLDADGQH
                     PPEMIPQMVRCWQEGADIVQCIREQASKSGVLKKVTSRGFYKVFNLLADTDIPEGAAD
                     FRLLDRQVVDALNTLPERVRFIRGLVHWVGFRQVSIPYSEKERLAGTTKYSLKHMFTF
                     ALNGITSFSVRPLRLSFVLSLFVILSIALYSGYVLFALAAGKDISPGWTSLVFIVLFL
                     GAAQLLVIGIASEYLARLYIEQKRRPVYIVRKPDSRREDA"
     gene            complement(4179..4658)
                     /locus_tag="PKN92_09540"
     CDS             complement(4179..4658)
                     /locus_tag="PKN92_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49076.1"
                     /translation="MDKRLLAAFFIFLGAFLVLPIAMQKYRQSQIPDAGGAAASGSYS
                     ASPASAGSATQFPELNEPPLLNEANLIGTEWQVQVEQYKIKVTLSAGGICYATHPLAK
                     AITGMDYIEGRWRVENNKFFVSTALGGQEKSVELRIAGNNLYHLSAKGRPERVERFR"
     gene            complement(4791..7382)
                     /gene="valS"
                     /locus_tag="PKN92_09545"
     CDS             complement(4791..7382)
                     /gene="valS"
                     /locus_tag="PKN92_09545"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNZ49077.1"
                     /translation="MELPKKYQSLEQENRWQKEWEEKALYAWDPSCSREETFAVDTPP
                     PTVSGSLHVGHLFSYSHQDFIVRFQRMTGKNIFFPIGWDDNGLPTERRVQNMYNVKCE
                     PHVPYDPELKTERGRKGTPLPISRKNFIELCDVVTKEDEAAFKHLWTRLGLSYDWSLE
                     YATIDEHCRRTSQASFLDMLEKDEVYQDNRPVLWDVDFRTAIAQAEVTDKEVAGAYHF
                     LRFGVEGSEEVLVIATTRPELLAACVAVMVHPEDARYRAYVGKKAVTPLFGVPVPIIA
                     DERANPEKGTGVVMVCTFGDQTDVEWWQEYKLPLRQILGQDGRLLPLTFVARSEKDAL
                     WISVNPELANATYGELAGLPAKKAQQRFIEIAEATPGVIDRPSEAITHAVKFFEKGDR
                     PLELIPARQWYGRIMDKKEALLEQGRKIQWHPEFMSKRYEHWVEGLNQDWCLSRQRFF
                     GVPIPVWYPLDEQGRCKYDEVIIPSPDSLPVDPLDDVPPGYESSQRDQPGGFTGDPDV
                     FDTWATSSLTPQIASRWVEDKDRHSRLFPMDIRPQSHEIIRTWAFYTIVKAYLHEGVI
                     PWKHVVISGWILDPDRKKMSKSQGNVITPEPLIDEYGADSVRYWAARARLGVDTAYDE
                     QVFKVGRKLCTKLFNAAKFAIGRFSEVERAALVPEAVTEELDRAFLAELRPLISRATR
                     AFEEFDYAQALSLVEDFFWTCFCDNYLEMAKPRTYDEELTAERLSACATLRISLSMLV
                     RMFAPFVPYITEEIWNWEFSKEEPSVHRAPWPALSEIAVVPAPASSKIWQAALMLTEQ
                     VRKAKADANLSMAAPVREVLVKAAPDWMEGVRAAAQDIIRMLRVETWNLVEDETVDSV
                     LSSAHLE"
     gene            complement(7515..8339)
                     /locus_tag="PKN92_09550"
     CDS             complement(7515..8339)
                     /locus_tag="PKN92_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ49078.1"
                     /translation="MAEALTDYRRLCVHTMTTKPLTLEEAITEYTRAGIPGITVWRHH
                     FEAMGAEEAGKRLQESGLRVVSLCRGGFFPASSAREREEARDQNRRIIDEAAAIGAPL
                     VVLVCGAVPGMPLAEARRQIVDGIAAILPHAAAAEVKLSIEPLHPMYADDRCAVNTLE
                     QANNMVSTLQSPWLGVTVDVYHCWWDPMLKSEIARAGNRIFSFHVCDWRTPTRDLLND
                     RGMMGAGCIDIRGIRGWVEEAGFKGPVEVEIFSDEYWQWDQRALIEQIKRSWLVYV"
     gene            complement(8377..9231)
                     /locus_tag="PKN92_09555"
     CDS             complement(8377..9231)
                     /locus_tag="PKN92_09555"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNZ49079.1"
                     /translation="MSDTLTKDEIQALLCPEGFFQTGIRGGTVWMRKDVSPDQLQQML
                     AHPQETLKETGKSKVSRLGHWVIKRRKSTGGSTALASLIRRETLRTPWYATHYLRGHG
                     VRVPEPLAYVEYGFSGIVSSTAFVFQYLERCKDVESYLFDFIREGADVEAVSGFLSNL
                     AQSINLVEKSGAWHSDLSGKNIYTGDGRVFYLIDLEAVDLKATYTQERRMKNHVQLYD
                     SFCDALTDTLLFPFIKEMTPDSIDLRLWMPEVRRIQEERRARVEKHWEKYGAPEKINP
                     LRALQRHQ"
     gene            complement(9235..9960)
                     /locus_tag="PKN92_09560"
     CDS             complement(9235..9960)
                     /locus_tag="PKN92_09560"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNZ49080.1"
                     /translation="MKVLAREEFCVKQALLYGEGCYPSGISGRGEVSFFPLADGGEGV
                     LRRCRRGGLIQLFLHNRYLFINRPLLEFEVHAEAQRRRLPVPLLLGVKWRQQGCTFSG
                     VLATRRMEGQDLDAWLCTAENPAEKHPEILHACGVMIRTCHDAGLFHADLNIKNIFIE
                     KGCPRLLDFDRACFYNALGDGQRARNLLRLRRSFEKKGHSREDFEMLLQGYGSVNIPR
                     WLQLLSLIKNSISDLFQRKRKIS"
BASE COUNT         2762 a         3074 c         2713 g         2458 t
ORIGIN      
        1 gcaacggcac ttcggttccc gtttcccgta cagcagcact gcgtgtcgta tgacgaatcc
       61 cctgctccga aaagtgtgtt tgtgtatcag tcaattccgt gtcgatcacg actccgggat
      121 tggaacgata ccacagcatc agagaactgt gcagtactcc gcaggagcct acaggccgcc
      181 cttcagccga ttttgccgga gacagacgca cggtgaagga tttggttgcg ccgggaggca
      241 ctgctgaaaa caaggcatcc ggctgagtga tctcctgctc cagatatttt tctgaaacgc
      301 cggagtttcg aagcgacttc cgttccactt gaggtatagg cgcatcaagc ggctcttcga
      361 gaaaaacagt tgatgccgcg gggtaaaact ctcttttgcc ggggagcgtg ccgacacaat
      421 attgctttgc caaccagaaa gaacgtttaa aaagaagatt gccgcgcagg ttttcccggg
      481 tcacttccca gtcctgcacc cctctatagg gtcccttatc gcggggcggc ccgctgagaa
      541 gatcacgaac agcgctcaaa tgcagcggat ccacaccatt catggaaaat tggagccggt
      601 agagcaggcg gttacagacc ggatcagtcc tgcgtctgtt gtcaatgggg acagcacagg
      661 gctccggtgc atcctgaata taatcccgga cttttgcctt cgccatatag ggaatgaaac
      721 tctggttcca ggcaaatgtt tctgcaaaaa gaagccccat cagcagcaga acgacgacag
      781 gacgcgcccg gtcattcagc ggaactgtcc cggcaaggag catcaggatg agtgccacaa
      841 gaggcagtac aatcacaata cgattgactg ttatatactg aatctccggc agccaggagg
      901 taatgggcgc aatcagcaca acggcgatga ggagcagtcc cactgcccgg gcgagagatt
      961 tgccccgatc tgccaggggc gcccgaatgg catccagccc gaatccggca aggagagaca
     1021 aaggaagcaa ggcgaaatca taggcgcgtg tataggcgga catggaaaaa ggcgtgactt
     1081 tttccagcag tgctcccagc ggcagcggcg gaccgaaact gcattcaaaa agaaccagaa
     1141 aaagccccaa aaacagaaaa ctgtctctcc tttttttcca ggtcagccct gcagctgaaa
     1201 gcaggagcgc tacgattccc gagttgcgca gtgtctccgc ttcatacttc acaccgccat
     1261 acagcacgag ttttttataa aaatcaagag gcgtccactt ccataagtcc gagtatttcg
     1321 gggttgcctg accaccggtg cgcaaggtgt aggagcccag ctcccaggaa gggataagtg
     1381 atacggcggc aagacccgat ccgacaagaa agatgatcgc catggcaatg ccattccata
     1441 gaaaaggccg gaaaaacgcc agacgtcccc cttcttcctg tcgctgccag tcgtggttca
     1501 gaagaaaata aaagagccca tagagggcgg ataccagccc catcagattg atgatctgca
     1561 gaaatccgcc gagaatactc agaccaagaa cggttccggc gaggagtgac agaaatactt
     1621 tgagagcaaa ctcccgggca tctattatct tttttatcag cagcagcagc aggggaagcc
     1681 aggccattgt tgtaataaaa tgatactcgc acatacgtct gaccatgagc gcgctgaaaa
     1741 gaaaagagag agctccggta agcgccgctg tcacactcat tttatgcgca cgcccgagaa
     1801 gataggtgca gacccccata aaaagaaaat gcaatcccat catgattgca agggatatat
     1861 tggtacgcgc gggagtcgga ttggatgtga gtagtgaccg gatcaggttg ggcggataaa
     1921 aaataaaact ttgaggatta cccacattag gcaggccaca ataggtcagc gggttccagg
     1981 caggaaattc gccattgtga atacagctgt ccacaaaaaa ggtcagaggt ccgtagtacc
     2041 ggtaaatgtc atacatgccg cccaacttgt ccagatcatt gaagagaata aactggaaag
     2101 taacggttgt ggcaaccgcc agagtcagca gaaccagaat ggcaatgatt gttttttgtc
     2161 tgaaactgtt taaggacatg actgagattt ttatcctgcg ttggtgtggc ataaaatgaa
     2221 acaaggcggt actgccgctg ccggaacagc aatccggtct cttatttctt tctggcgtaa
     2281 actgtcatca aatcgaaaag attcaagggg taattaatgc gtccgagcgg tgtgcgggcg
     2341 accaaaggcc ggataagaga aaacactctg tgcttctgtg ccagttttac aagcgtagtg
     2401 ttaacattgc gccagaaagg cggataagaa aaacgcactt cctcaaaacc aagccgggca
     2461 agcagctgcc ccatggagtg ctgcgtaaag taatgcacat gggtcggcgg gtgaatcagc
     2521 cgccagtgcc gcccccgcac tctagcccat cgcgatccta tatctccagt gctcagcgcc
     2581 agcgttccac ccggtattag ttcggaaaag gccttttcca gatacagatc aggccgcgcc
     2641 atgtgttcaa ctgtatccca caggcagatc acatcaaatg ggcccccttt ctccattttc
     2701 aaataatccc cgtataccgc cttcaccccg cgggcggcaa ctccgctttc aacggcatca
     2761 gcggcaatgt cacagcctgc agcattgaaa tgcgtctgcg ccgcttcgag aaaaaaaccg
     2821 taggcacatc cgatttccca caagtcaccg ccttcaggat ggcgctttgc cagatccgcc
     2881 aggcgtttca tgaaattata tcgaagcgcc agtccttcct cctcgtagtc ctcgtattcg
     2941 cctcctttga aatagccccg tgtatacagt tcatggaatt tttcgggcgc ccaatccaaa
     3001 acagcacgga tgtgcccgca cttgtcacag cgttcgagcc cttcataaac ggcggtatgc
     3061 ggaaaatgcc cgcagcagat acaggaaata cgctctgctt tatccccttc ggaaacatat
     3121 tgttcaaagt cgtccataaa ttgccttatc tgaaaatcat taaaaactat gcatcttcac
     3181 ggcgggaatc gggcttgcgc acaatataga caggccgtcg tttctgttct atgtagaggc
     3241 gagccaggta ctcactggca atcccgatga ccagcagctg cgccgctccc agaaaaagaa
     3301 caataaaaac cagacttgtc catcccggag aaatgtcttt tccggctgcc agcgcaaaga
     3361 gcacatagcc actgtataaa gcaattgata agatgacaaa aagggagagg acaaaagaga
     3421 ggcgcagcgg ccgaacagaa aaactggtga tcccgttcaa cgcaaatgtg aacatatgct
     3481 tcaaactgta cttggtcgtt cccgccagcc gttctttttc cgagtaagga atggaaacct
     3541 gcctgaaacc cacccagtga acaagccccc ggataaaacg cacccgctca ggaagagtgt
     3601 tcaatgcatc gacaacctgg cgatccagca ggcgaaaatc agcggcacct tcggggatat
     3661 ccgtatcggc aagaagattg aagactttat agaacccgcg ggacgtcact tttttaagaa
     3721 cgccgctttt tgaagcctgc tcacggatac actggacaat gtcggcacct tcctgccagc
     3781 atcgaaccat ttgcgggatc atttccggcg gatgctgccc gtcggcatcc agcgtgatga
     3841 cggcatggct tgcccgtgca cattccagcc cggcgcatag tgccgccata tggccgaaat
     3901 ttcgggaaaa atgaaaaaca cggacagccg gatatctttg agtcagagaa tccagacgca
     3961 tgcctgtcgc atcagaactg ccgtcgttaa tccatacaat ttcgacagtc ccctcaatct
     4021 gctccgccac agcaagaagg cgctctgtca ttgccacaac actatcttct tcgttatagg
     4081 caggcagcac aaaagaaaat tttggagtca tactcatata gcactattgc ccggtaccct
     4141 tgcacccctg cataagactc cccttcatca tgcttttctc agcggaagcg ttccactctc
     4201 tccggtctgc ccttggcgct taggtggtag agattattgc cggcgatacg aagttccacc
     4261 gatttttcct gaccgcccag agcggtgctc acaaaaaact tgttgttttc cacccgccag
     4321 cgcccttcaa tataatccat tccggtaatc gcttttgcca gagggtgggt agcatagcaa
     4381 attcctccgg cagacagggt aactttgatt ttgtactgct cgacctgcac ctgccattct
     4441 gttccgatga gatttgcttc atttaaaaga ggcggttcat tcagctcagg gaactgcgtg
     4501 gcggaaccgg cagaagccgg actggcggaa taacttcctg acgctgccgc cccgcctgca
     4561 tcaggtatct gtgactgtct gtacttctgc attgcaatgg gaagcaccag aaacgcacca
     4621 agaaaaatga aaaaggcagc cagcaaccgt ttgtccatag gcccattaac ccttttttgt
     4681 tctggaaccg aagtccctgc cggttcacgc aagaagcctg attttcttat ttcattttac
     4741 tcttgattct tcacaagaat caacccggat tctgtaaaag aggaaaaaag ctattccagg
     4801 tgcgctgagc ttaaaacgga atccacagtc tcgtcctcaa ccagattcca ggtttcaacc
     4861 cgaagcatcc ggataatatc ctgtgcagca gcacgcaccc cctccatcca gtcaggtgcc
     4921 gctttcacca gcacttcgcg gacaggcgcc gccatgctga ggttggcatc ggcttttgcc
     4981 ttgcgcacct gctcagtcag cataagggca gcctgccaga tttttgacga agcgggcgca
     5041 ggtactacag cgatttcaga aagagcaggc cagggtgccc ggtgtacaga gggctcttct
     5101 ttcgaaaact cccagttcca gatctcttca gtaatataag ggacaaacgg tgcaaacatt
     5161 cggacgagca tactcaggct gattcgaagc gttgcacagg cggagagtcg ttcagcagtc
     5221 agttcttcat cgtaagtacg gggctttgcc atttccagat agttgtcgca gaaacaggtc
     5281 cagaagaaat cctccacaag actcaaagcc tgcgcataat caaactcctc aaaggcacgg
     5341 gttgccctgc tgatcagcgg gcgcagctcg gcaagaaaag cacgatccag ttcttctgta
     5401 acagcttccg gtaccagcgc agcccgctcc acctcgctga agcgtccgat agcaaacttg
     5461 gcggcattaa acagcttcgt acaaagtttg cggccgacct tgaaaacttg ctcatcataa
     5521 gcagtatcca caccgaggcg tgcccgagcc gcccagtatc ggacactgtc tgctccgtac
     5581 tcatcaatca gcggttctgg tgtaatgaca ttcccctggc tcttcgacat ctttttccga
     5641 tccggatcca gaatccagcc gctgatcacc acatgtttcc agggaatcac accttcatgc
     5701 aaataggcct tcacaatcgt gtagaaagcc cacgtgcgga tgatctcatg actttgcggc
     5761 cggatatcca tgggaaacag acggctgtgc cggtctttat cttcaaccca ccggctcgca
     5821 atctgcgggg tcagtgagct ggttgcccag gtgtcgaata cgtccggatc gccggtgaag
     5881 ccgccgggct gatcacgctg gctgctttcg tacccgggcg gcacatcatc gagaggatca
     5941 accgggaggc tgtccggcga tggtataatc acttcatcat atttgcagcg gccctgctca
     6001 tccagcggat accagaccgg gatcggcaca ccgaaaaaac gctgccggct caggcaccag
     6061 tcctgattca gcccctccac ccagtgctcg tagcgcttgg acataaattc cggatgccac
     6121 tggattttac gcccctgctc caacagcgct tctttcttgt ccatgatcct gccataccac
     6181 tggcgggcag ggatcagctc gagcggtctg tcgccctttt cgaaaaactt caccgcatgg
     6241 gtaatcgctt ccgaaggtct gtcaatcacg ccgggtgtcg cctcagcgat ctcaatgaat
     6301 cgctgctgtg cctttttcgc cggcagcccc gccagctccc cgtaggtcgc attggcaagc
     6361 tcggggttga cactgatcca aagggcatcc ttttcagacc gggcaacaaa agtcagcggc
     6421 agcagcctgc catcctggcc cagaatctgg cggagcggca gtttatactc ctgccaccat
     6481 tccacgtcgg tctggtcgcc gaaagtgcag accatcacca cccctgttcc tttctcaggg
     6541 ttggcacgct cgtcagctat gatcggcaca ggcacaccga aaagaggcgt cactgccttt
     6601 ttacccacat atgcgcgata acgggcatcc tccggatgca ccatcacagc gacgcaggca
     6661 gccagcagtt ccggccgggt cgtggcaatg accagcacct cttcggagcc ctccacaccg
     6721 aaacgcagaa aatggtaagc ccccgctact tccttgtccg tcacttccgc ctgcgcgata
     6781 gccgtccgga aatcaacatc ccacagcacc ggccggttgt cctgatagac ctcgtccttc
     6841 tccagcatgt ccagaaaact tgcctgcgag gtacgccggc aatgttcgtc aatggtggca
     6901 tattccagtg accagtcgta actgagaccc agccgagtcc acaaatgctt gaaggcggct
     6961 tcgtcttctt tggtcactac atcgcacagt tcgataaagt ttttgcggct gataggaagc
     7021 ggtgtgcctt ttcttccccg ttccgtcttc agctccgggt catagggaac atgcggctca
     7081 catttgacgt tgtacatgtt ctgtacgcgt cgttctgtgg gcagtccgtt atcatcccag
     7141 ccaatgggaa agaatatatt ttttccggtc attctctgaa aacggacgat aaaatcctgg
     7201 tgggagtaac tgaagagatg accgacatgc agtgaaccgc tgacagtggg cggcggtgta
     7261 tcaacggcaa atgtttcttc gcggctgcat gagggatccc aggcgtataa tgccttttct
     7321 tcccattctt tttgccagcg gttttcctgt tccagagatt gatatttttt cggcagttcc
     7381 atggagtcct ccgggagagc gtatgggggt tttgcgggta tacgacggtt ttcatgcact
     7441 gacagtacac cggcagcgtg acgaatctgc tgtattatac acgaagcacc gaaaggctga
     7501 aaaacccgct tccgctatac ataaacaagc caggaccgtt tgatctgctc gatcagggca
     7561 cgctgatccc attgccagta ttcatctgaa aaaatctcca cctcaacagg gcccttgaac
     7621 ccggcttcct ccacccagcc acggatccct ctgatgtcaa tacagccggc gcccatcatg
     7681 ccgcggtcat tcagcagatc acgggtcggc gtacgccagt cgcagacatg aaaagaaaag
     7741 atgcggttgc ccgcccgggc aatttccgat ttcagcatgg gatcccacca gcaatggtac
     7801 acatctaccg tcacaccaag ccatggcgac tgcagtgtgc tgaccatgtt gttcgcctgt
     7861 tccagcgtat tgacagcgca gcggtcatcg gcatacatcg gatgcagcgg ctcaatggac
     7921 agtttcactt ccgctgcggc agcatgaggc aggatagcag caataccatc cacaatctgc
     7981 ctgcgcgctt cggcaagcgg cataccggga acagcaccgc aaacaagcac aaccaacggc
     8041 gcgccaatag ccgcagcttc gtctataatg cgccggttct gatcgcgtgc ctcctctctc
     8101 tcccgggcgc tgctggcagg aaaaaaacca ccccggcaca aactcactac ccgcaaaccg
     8161 gattcctgca gtcttttccc cgcttcctct gcacccatcg cctcaaaatg gtgccgccat
     8221 accgttatac ccggaatgcc cgcccgggtg tattccgtaa tcgcttcctc cagcgtaagc
     8281 ggctttgttg tcatggtatg cacacacagc cgacggtaat ctgtcaatgc ttctgccata
     8341 atctgcattc ccgggatttt tctcatcaca gtgcggttat tggtgacgct gcagcgcccg
     8401 aagtggattg atcttttccg gtgccccata cttttcccaa tgcttttcca cccgtgcgcg
     8461 gcgttcttcc tgaatccggc gcacctccgg catccacagc cgcagatcaa tagaatccgg
     8521 cgtcatttct tttataaaag gaaaaagcag ggtgtcagtc agggcgtcgc aaaaagaatc
     8581 ataaagctgc acatggtttt tcatgcgtcg ctcttgtgtg tacgtcgctt tcaggtcgac
     8641 agcctcaaga tcaatcaggt aaaacacacg gccgtcgccg gtatagatat ttttgccgga
     8701 aagatcacta tgccacgccc cgcttttttc aacaagattg atgctttgcg ccaagttgga
     8761 caaaaaaccg gagactgctt caacatcggc gccctctctg ataaaatcaa aaagataact
     8821 ttccacatct ttgcagcgct caaggtattg aaatacaaaa gctgtgctgg aaacaatgcc
     8881 ggaaaaacca tattccacat aagccagcgg ctccgggacg cgaacaccat ggccacgcag
     8941 ataatgggtc gcataccagg gcgtacgcaa tgtttcacgc cgtataaggg atgccagtgc
     9001 cgtgctgccg ccggttgatt tacggcgctt gatcacccaa tggccgagcc tgctgacttt
     9061 ggacttcccc gtttctttca gcgtttcctg agggtgggca agcatctgct gtaattgatc
     9121 cgggctgaca tctttgcgca tccataccgt tccgccacga atgccggtct gaaaaaaacc
     9181 ttcaggacaa agcagcgcct gtatttcatc tttggtcaaa gtgtctgaca tgtctcagct
     9241 gatctttctt tttctctgaa acaaatccga tatgctattt tttatcaaag acagcagctg
     9301 cagccatctg gggatattta cggaaccata accctgcagc agcatttcaa aatcttcccg
     9361 gctgtgtcct tttttttcaa aagaccggcg cagtcgcagc agattccggg cacgttgccc
     9421 gtctcccaga gcattataga aacaagcgcg gtcaaagtcc agaagccgcg ggcaaccctt
     9481 ctcaatgaaa atattcttta tattcaaatc agcgtgaaac agccctgcgt catggcaggt
     9541 gcgaatcatg acaccgcagg catgcagtat ttccggatgc ttttccgccg gattttctgc
     9601 tgtgcagagc caggcgtcta ggtcctgccc ctccattcgc cgggttgcga ggacgccgga
     9661 aaaagtgcag ccctgctgtc gccatttcac acccagcagc agcggcaccg gaagacggcg
     9721 acgctgcgcc tctgcatgca cctcaaattc gagaagagga cgattaataa aaagatagcg
     9781 attatgaaga aataattgaa taagcccgcc ccgccggcac cgacgcaaaa caccctctcc
     9841 cccatcggca agggggaaaa aagaaacctc tccgcgaccg gaaattcccg aaggataaca
     9901 gccttccccg tagagcagcg cctgcttcac acagaactcc tcccgagcaa ggactttcat
     9961 atgctcattc cgatcggata aataaataaa tgacgtatca cccaatactg tcagcccgta
    10021 cttcctgaga taaaacgaat tgaaaaagag cgttctgtcc ttcgttgcag gaacaatctt
    10081 ccctgaaaat gaaatgatag cacagcaccg cccgatgaaa cacacagaac cagaataaag
    10141 atacttgtga actcactatc gaaatgcatt tttccatatt ggcacacaag ccggttaagg
    10201 cgaaaacagt cactcggatt cggtgcggaa atgcgtcttg ggacatacct cctccatcag
    10261 tcctacacac ggcgctgtca ttgctccctt ccgccgttac gtccaccctc aggcgtcatg
    10321 gctgcctccc ccggcgtcat tcctgcgcag gcaggaatct taacgcgagg tgctatttat
    10381 aagagtcatg ctgttgccgt caactacgcg ggaaagtctt tttttgtgtt tgtatgtctt
    10441 gaaggctttg gtgtggatca gggtgatatc taagtcaaga cgtccatacc ctgattaaga
    10501 ttcctgcctt cgcaggaatg acggggggga gcaggaatga cgggggggag caggaatgac
    10561 ggagggggga ggcagaaatg acagagagtg cggcatgccg gggagcgcag ggacggcgga
    10621 gagtgcggtg atgacgatgc gtacagaagt ggcgcccgag aaatgcaggt cttccctggg
    10681 aacgccaatc tccgtattgg cacacaagcg gatcatgacg aagatagtca ctcggatccg
    10741 catcggtatt atgccccggg acatactccc ataaacgtag tcctcacctg cctcttgcca
    10801 gtacggagat tggcgttccc aggctggcga attttatgtt tttggttaca gtattttgtt
    10861 gcagaatcca tacaagcccc aaataacagc cagtaatttg ctcacaagca tgtaattgcc
    10921 agtatatctg ttcaaattcg accgatgatg gcaggttatg tcaagaagtt tctgtaaatt
    10981 agttttcctg gtgaacagtg cgttgtt
//
LOCUS       DAOPWB010000103        10919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6076_length_10919_cov_5.279823, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000103 DAOPWB010000000
VERSION     DAOPWB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6076_length_10919_cov_5.279823"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1677)
                     /locus_tag="PKN92_09565"
     CDS             complement(97..1677)
                     /locus_tag="PKN92_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49081.1"
                     /translation="MASTLCLPMACQSARVVATDPGVTTVPLQESMKRAADCCLAWLN
                     PAYRFLPAGGYEIAHDTGRWWDAMLRYEACTGERISADLEEAMLANAKEMTQNRVALL
                     TNIFGPPESVVINPHNFRESMQTYTLLVKYRKNEWAFEQGKTMVQALIGLLDDDGQMN
                     YGRLIEMMDGLPLTGDPMMRPLAASGEWFDSTPTTGRAIEPIVQFHDATGYAPAMELA
                     ERLCEIHFKQIIEPDGELCEEIRSGKQRSHNHSYNGMLRGMLLFGRKTHNKKYIDAVL
                     NTYRNSVLRHCITESGYTPHDLGLFLFVNEHGDPIGEHSSPADIMQIALWLAQHTKDA
                     ALYNDIERILRTRLLPSQINNPNVPRDHGAWGCYANPYDFASILDVFAAVLHALTEVS
                     KGAISQAEDKTLSAHLHFDCDLPELALRSQRGEEATVTITPKVKNALRIRVPDWATRS
                     SVSLTQISDSIPLVWDGSYLVVGRHNVRPGRTITLKHDLPARETVEETPVAKIPYKLF
                     WKGDTIVGSSPAVPIYSA"
     gene            complement(1619..2128)
                     /locus_tag="PKN92_09570"
     CDS             complement(1619..2128)
                     /locus_tag="PKN92_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49082.1"
                     /translation="MLFLPNFQRNSRELQSDDEHHKPQRQDAQGKKNVTYYFFLFHHT
                     IYSDFSDFLTFFCRKGAAGTKRRGGVGNHKFPKKYTWESVRLSKKAMINTSKFYLYFS
                     SHLQAIAFFFPGQYVYTNYAVFILLCLLAIEKGEDDAYYQKVFFKADSFGKHVMSAHG
                     LPVRTCSCD"
     gene            2327..3691
                     /locus_tag="PKN92_09575"
     CDS             2327..3691
                     /locus_tag="PKN92_09575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNZ49083.1"
                     /translation="MFDEEKLAAFLEFSPSARETYALLNGELIIKIDEKYKVIVPECY
                     GLLKAVMTPLKQRIFQYMLKHSSVFESARLIIQEVICDHQIRSSSVSVFGENKINFTC
                     CPSSLTDSNEKSVFIFAKVHPALLYQGKENSDSESRWLVPGYKLKGNFKSQKGDFYTT
                     NQYGFHDSFFEKTKAENTYRIMCVGGSTTEEGPSVDETYPKLLQKKLRVAFPGKELEV
                     LNAGTPANAAFCHLLRFNEYADFDPDMTILHIGVNDTLFYYDMDDWTFPAEKLRFLGS
                     FFSRLLARDIQHFSRQHYENMGVSLELFIQMALKRNIKVCLASIACPQLQKLNNEEWQ
                     YLKYNANTTWEKPLFDLPVYEERIQASNHLLFQLARKYDLPYLAIAENINAGMEFFTD
                     FCHMSPAGIEYKAQVFYEQLLPLLQKEFMAQRQELNYLEGGRFLDGSLKMQFETVSCG
                     VYWQ"
     gene            complement(3644..5848)
                     /locus_tag="PKN92_09580"
     CDS             complement(3644..5848)
                     /locus_tag="PKN92_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49084.1"
                     /translation="MLFALRFLCAMAVTFTLSHTVVLAAPAKASAKAPAKATTQAPAQ
                     KLPWTLREHYTFSGGPLRLDYAKDNGEWRIKLFQEDSEQARMNPDLILKEVRFSIELA
                     DGRVLTNDMFGAAGETFLDRGPAVWEVAGEGTHYAIQFQPLEGLSVEHAVTMLKQWNF
                     LLITLKVKNISENPISIQKIVTAEIPAGSLCGLSEQAEIGTCNMVFRGGHPVFSPEGL
                     SSSLRIYDPVRQVIVNIALIPQGKAASGIFSTRESGQWHGRIESDFSPAKPLAPGASL
                     EADPLWLSLGTAPALQDSQYSYLLYHFCKEGTTLDQPRAWVTVSDTAGLSRLKSEAGT
                     AAALGITHALIPGNWESKPGSLEGGAPLYPRNINEAASALQAAGVTPGITLDALPAKS
                     GGGHTAKSADGQLWNNPCVPEGKAAVKDRIRTLYSKGFGFFALEASAIPDDVLLAFGI
                     SRAEADTAAFSAAVEAVQGTKAAVLPAAASALPLNRDAWLAAASAAARIREGDIGIAP
                     VGVLLNTDQTPDFETRLALQMWPGPFQFVDAPSGSAGRFVSALLARPPFRARPQDPCR
                     TAPLIWLTRAGININPYVGEAIMQFSGASSWNVSDLECFNATGSLPVVWQDTEGKIAR
                     HDGVVIPAAAHATTLGLLNVLPEPMFCGISGEPMLGLYQAKKVQWDASKKILAGNLEG
                     PFMKDAVAVFYLPNGMQPRSASLGGKSVRPQVSENWLLLPIDPAGNSFELHF"
     gene            6246..6845
                     /locus_tag="PKN92_09585"
     CDS             6246..6845
                     /locus_tag="PKN92_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNZ49085.1"
                     /translation="MNNLTIPISCIGGDGYEFEECIPVESIQPPDTPAVTVGSVCISG
                     RFTKFRESYTFRGRVTGAYHGTCCQCLASADVPFCVQVRWIFEKISGNVLKEISRSQE
                     ETDFFYSHPAKNASAPAAQEDVICLAPYVWEEVVFAEPYTYVCGPDCKGVCGRCGTNL
                     NHNDCGCDKEKESQHLNDNQGLSALAKMFPELQSDKNKE"
     gene            6852..7034
                     /gene="rpmF"
                     /locus_tag="PKN92_09590"
     CDS             6852..7034
                     /gene="rpmF"
                     /locus_tag="PKN92_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNZ49086.1"
                     /translation="MALPKRRTGKAKKGMRRSHHALTAPNVVECPDSGEAKLPHRVCL
                     KTGFYKGRVVVKPQND"
     gene            7101..8231
                     /gene="plsX"
                     /locus_tag="PKN92_09595"
     CDS             7101..8231
                     /gene="plsX"
                     /locus_tag="PKN92_09595"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNZ49087.1"
                     /translation="MRIALDAMGTDNPPGPEVEGAVLASLNSDVEVILVGDEDLLKEK
                     LEAFPKKGNVSIRHAPEAITPLEPPVIAVRKKKHSSMHVAMRMVKEGEAQAFISAGNT
                     GAIMVATRVILGSIPGVARPALSQTFPTLQGRTVVLDLGANVDCTARHLCEFAEMGVA
                     YSRHALGVENPRIALLNIGEENVKGGAIAREAYRTLSKASHLNFIGNIEPRPLCEGKA
                     DVVVCDGFIGNLFLKTSEAVAYFMGKMVREHFESSNMSKIGAVLARKALKQIKHKMDP
                     NEYSGAPLLGVDGTVIVLHGASTVRGIENAIYGTQHALENHLLEHIRENIQTLKENGG
                     VNGSAQDALSADSMAGLGLQSETEDPPEAAVFSSAESSNLLQ"
     gene            8328..9062
                     /gene="fabG"
                     /locus_tag="PKN92_09600"
     CDS             8328..9062
                     /gene="fabG"
                     /locus_tag="PKN92_09600"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNZ49088.1"
                     /translation="MSSLKGKIAVITGGTRGIGYAVARLFIERGAQVALCGRSEESAS
                     AAAQRLGSSCIGIGCDVSNPAAVDALIGRVAATWGTVHILVNNAGSTDDGLLIRMKDE
                     RLERLIQTNLSSVFYTCRATAAMMMKQRYGRIINIGSVAGLRGNAGQCAYAAAKAGLI
                     GFTKSYAREMASRNITVNLVAPGFIDTEMTASFPEAQRDALLSRVPLQRPGTAEEVVA
                     AVAFLASDEAAYITGATLTVDGGLGT"
     gene            complement(9075..9950)
                     /locus_tag="PKN92_09605"
     CDS             complement(9075..9950)
                     /locus_tag="PKN92_09605"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNZ49089.1"
                     /translation="MNRRCFLQSAASAAASVSLCPATFSDVGNNPTFSDSGMRFSAPL
                     EWFPGLRPEDKLEHVARWGLAAYEWLWPIGDADKIREKACALGLEISCMAGTGAIAEG
                     WMVAPEDHDKVFEMLRQRIAMAKQLNCRRLIGLTGNSLKGTSAEAQTESVITCLKRLA
                     PLLEDSGMILVMEALNPLVDHKGFFMTRTDQTIEILQAVGSPNVKMLFDIYHQQITEG
                     NVIRNIRENIAHIGHFHVADNPGRNEPGTGELNYTNIFRAIRESGYTDFIALECGHST
                     GNYENTLQATLKCLL"
     gene            complement(10009..>10919)
                     /locus_tag="PKN92_09610"
     CDS             complement(10009..>10919)
                     /locus_tag="PKN92_09610"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ49090.1"
                     /translation="LKTLDLHPGDEVITTPFTFFATAGAIVNAGGIPVFADILPDTFT
                     VDPQSVRQLITQKTRCILPVHLYGQCADMDALTGIATEYNLPIIEDAAQALGTRMNNR
                     QPGNWGIAAALSFYPTKNLGAAGEGGAILTNDDALAEKLRMLRCHGASALYQHALVGT
                     NSHLHTIQAAVLAVKLKYLAQWNEKRREKAAYYTQCLKDVPGIIPPVEQDGMYHVYHQ
                     YVIRIPQRDRARQLLADREIGSGVFYPLPLHRQPCFQAYVSAKASCPEAERACTEVLA
                     LPVFPELTTEQQDQIIEVLRNHCASL"
BASE COUNT         2684 a         2645 c         2841 g         2749 t
ORIGIN      
        1 acatagatga aacgccggtt cttgtaatct aagaaccggc gtttctgttt ttaacagcag
       61 ataatgtatt tatggaagac gtgttttaga agcttctcag gcgctgtaaa taggaactgc
      121 cgggctgctt ccgacaatgg tatccccttt ccagaaaagt ttgtaaggga tctttgccac
      181 tggtgtctct tccacagttt ctcgtgcggg aagatcgtgc ttaagtgtga ttgttctgcc
      241 aggtcttacg ttatgccggc caacaaccag ataagagccg tcccagacca gcggaatcga
      301 atcactaatc tgtgtgagtg acacagaaga acgagttgcc cagtcgggca cacggatgcg
      361 gagtgcgttt ttaaccttcg gagtgattgt cacggttgcc tcttcgccgc gttggctgcg
      421 cagggccagt tccggaagat cgcagtcgaa atgcaggtgc gcagaaagtg ttttgtcctc
      481 tgcctggctg atcgccccct tgctgacttc agtcagtgcg tggagtacgg ctgcaaatac
      541 gtcgagaatg ctggcaaagt cataagggtt ggcatagcag ccccaggcac catggtcgcg
      601 ggggacattg gggttgttga tttgcgatgg caacagacgc gtgcggagga tgcgttcaat
      661 gtcattgtat aaagcggcat cttttgtgtg ctgggcgagc cataacgcta tctgcatgat
      721 gtctgcggga ctggaatgtt ctccaatcgg atctccgtgt tcgttgacaa acagaaaaag
      781 gcccagatcg tgcggcgtat atccgctttc ggtaatacag tgtctcagga cactgttgcg
      841 gtacgtgttc aatacggcgt ctatgtattt tttattgtgt gttttccgcc cgaagagcag
      901 catcccgcgc agcataccat tgtacgagtg attgtggctt ctttgtttcc cggatcgaat
      961 ctcttcgcag agttcgccat cgggttcgat gatttgctta aagtgaattt cgcaaagacg
     1021 ctctgcaagt tccatggctg gcgcatagcc ggtggcatcg tgaaattgta caatgggttc
     1081 aatcgcacgg cctgtcgtcg gagttgagtc aaaccattcg cctgatgccg ctagggggcg
     1141 catcatgggg tcgccggtta gcgggagccc gtccatcatt tcaatgaggc ggccataatt
     1201 catttgcccg tcatcatcaa ggaggccgat aagcgcctga accattgttt ttccctgctc
     1261 aaatgcccac tcgtttttac ggtatttcac cagcagtgta taggtctgca tactttcacg
     1321 gaagttatgc gggttaatga ctactgattc aggcggaccg aagatattag taagcagggc
     1381 aacccggttt tgtgtcattt cttttgcatt ggcaagcatg gcttcttcaa gatccgcaga
     1441 aatccgctcg cctgtacagg cttcataacg aagcatcgca tcccaccagc ggccagtatc
     1501 atgagcgatt tcatatccgc cggcagggag aaaccggtac gccggattca gccaggcaag
     1561 acagcagtct gcagcacgct tcatggattc ctgcagcgga actgttgtaa cgcccgggtc
     1621 agtcgcaact acacgtgcgg actggcaggc catgggcaga cataacgtgc ttgccaaagc
     1681 tgtctgcttt aaaaaaaacc ttctggtaat acgcatcatc ttctcctttt tcaatggcta
     1741 aaagacacaa aagtataaaa acagcgtaat tagtataaac gtattgcccc gggaaaaaaa
     1801 acgctatcgc ctgcaggtga gatgaaaaat agaggtagaa cttggaagta ttgatcattg
     1861 ccttctttga taaacgaacg ctttcccaag tatacttctt tggaaatttg tggttgccga
     1921 caccaccccg ccgttttgtt ccagctgctc cttttctaca gaaaaatgtc agaaaatcac
     1981 taaaatctga atagatcgtg tgatggaaaa gaaaaaaata ataagttaca ttcttttttc
     2041 cttgagcatc ttgcctttgt ggcttatggt gctcatcatc gctctggagc tcgcggctgt
     2101 ttctctgaaa gtttggcagg aaaagcataa tcccctcatt agagccttta taacagaagg
     2161 atatatccct caaaaggctt cgctagagac aatttcggac catcatttcg ctaagcctcc
     2221 cggaggctga tcaaaagaca ggcaaattaa tacatgcgat ttctattcag attcacaggt
     2281 tcaattattg ctgaatgagg gtcctctgca aactgatagc attcaggtgt ttgatgaaga
     2341 aaaactggct gcattcttag aattttcccc ttccgcacgg gaaacctatg cattattaaa
     2401 tggcgagctt ataataaaga ttgacgaaaa atataaagtc atcgttccgg aatgctacgg
     2461 tctgcttaaa gcagtgatga cgcccctgaa acaaagaatt tttcaatata tgctgaaaca
     2521 ttcaagtgtg ttcgagtccg cacgtcttat aattcaggag gttatttgcg atcaccaaat
     2581 ccgaagttct tctgtttctg tttttggtga aaataagata aattttacct gctgcccttc
     2641 gtctttgacc gatagcaacg aaaaatctgt ttttattttc gcaaaagtcc atcctgcact
     2701 tttgtatcaa ggcaaagaaa atagcgatag tgagtcgcgc tggctggtac cgggctacaa
     2761 gctaaaaggc aacttcaaaa gccaaaaagg agatttttat actacaaatc aatatgggtt
     2821 tcatgattcg ttttttgaga aaacaaaagc ggaaaataca taccggatta tgtgtgtcgg
     2881 cggttcgaca acagaagaag gtccatcagt tgacgaaacc tacccgaaac tgctgcagaa
     2941 aaaattaagg gttgcttttc ccgggaaaga attggaggtg cttaatgcag gcacgcctgc
     3001 aaatgccgct ttctgccatt tgctccgttt taacgagtat gcagattttg atcctgatat
     3061 gaccattttg catattggcg ttaatgatac tttattttat tatgacatgg acgattggac
     3121 ttttccggca gaaaaactca ggtttttagg cagtttcttt tcccgcctgc tggcgcgcga
     3181 tattcagcat ttcagtcgcc agcattacga aaatatgggg gtttcgcttg agctctttat
     3241 acaaatggct ctcaaacgta atattaaggt gtgtcttgca tccatcgcct gcccgcaatt
     3301 acaaaaatta aacaacgagg aatggcaata tttaaaatat aatgccaata ccacctggga
     3361 aaaaccatta tttgatctgc cagtttatga agagcgtatt caggcgagca atcaccttct
     3421 ttttcagctt gcaaggaaat atgatctgcc ttatcttgcc atagccgaaa atataaatgc
     3481 tggaatggag ttcttcacag atttttgtca tatgagtccg gcaggtatcg aatacaaagc
     3541 gcaggtgttt tatgaacagc tcctgccgct gcttcagaaa gaatttatgg cgcaacggca
     3601 ggagttaaat tatttggagg gggggcgctt ccttgacggc tctttaaaaa tgcagttcga
     3661 aactgtttcc tgcggggtct attggcagta gcagccagtt ttcgctgact tgcgggcgga
     3721 cagatttacc gccgagagaa gcggagcgtg gctgcatccc gttcggcaga taaaagaccg
     3781 caacggcatc tttcatgaaa ggtccttcaa gatttcctgc cagaattttt ttggaagcgt
     3841 cccattgtac ttttttcgcc tggtacagac ctagcatcgg ttctcctgaa ataccgcaga
     3901 acataggctc tggcagcacg ttgagaagcc ccagagtagt agcatgggcg gctgcaggaa
     3961 taacgacgcc gtcgtgacgt gcgatttttc cttctgtatc ctgccagaca acgggcaggc
     4021 tgcccgtagc gttgaaacat tcgagatcgc tcacattcca cgaagatgcc ccggaaaact
     4081 gcataatcgc ctcgccgaca tatggattga tgttgatgcc tgctctggtc agccagatca
     4141 gcggtgcggt acggcaggga tcctgcggtc tggcacggaa aggcgggcgg gcaagcagtg
     4201 cggaaacgaa acgtccggca ctaccggaag gcgcatcgac aaattgaaag ggtcccggcc
     4261 acatctgcag cgccagacgt gtctcgaagt ccggcgtctg atcagtatta agaagaactc
     4321 ccacaggggc gataccgatg tcgccttctc tgatacgggc agcagcagaa gcggcagcaa
     4381 gccaggcatc ccggttgagg ggaagggcag aggctgcggc aggcagcaca gcggcttttg
     4441 ttccctgcac tgcttccacg gcggcggaga aagcggcggt gtcggcttcc gcccgggaaa
     4501 ttccgaatgc cagcaggacg tcatccggaa tggccgatgc ctccagtgcg aaaaagccga
     4561 agccttttga ataaagcgta cgaatcctgt ctttgacggc tgctttccct tcgggaacgc
     4621 aagggttgtt ccacagctgt ccgtctgcac ttttggctgt atgcccgccg ccacttttag
     4681 ccggaagtgc atcaagcgtt atgcccggag tcactccggc agcctgaagc gccgaagcag
     4741 cctcatttat atttcgcggg taaagcgggg cgccgccttc cagagatccg ggcttgcttt
     4801 cccagtttcc agggatgagc gcatgggtga tgccgagcgc cgctgcagtg cctgcctcac
     4861 ttttaagccg tgacagaccg gcggtatcag aaacagtgac ccaggcccgc ggttgatcca
     4921 gtgttgtgcc ttctttgcaa aaatggtaga gcaaataaga gtactgggaa tcctgcagtg
     4981 caggtgctgt gcccaggcta agccaaagcg gatcagcttc cagactggca ccgggggcaa
     5041 gtggcttcgc tggcgaaaaa tcgctttcaa tacgtccgtg ccattgtccg ctttctctgg
     5101 tcgaaaagat ccccgaggca gcttttccct gcggaatcag ggcaatattt acaataacct
     5161 gacggacagg atcataaata cgtaaggatg aggaaaggcc ttccggagaa aacacagggt
     5221 gcccgccccg gaaaaccata ttgcatgtgc caatttctgc ctgttcactc agaccgcaga
     5281 ggctcccggc cggaatttcg gcagttacaa ttttttgaat actgatggga ttttcgctga
     5341 tatttttaac tttgagcgtg atcaaaagaa aattccactg ttttaacatg gttacagcat
     5401 gctcaacaga caatccttcc agcggttgaa actggatggc gtagtgcgtg ccttctcctg
     5461 ccacttccca cacggctggg cctctgtcga ggaatgtttc tccggcggca ccgaacatat
     5521 cattggtgag cacacgaccg tctgccagct cgatagaaaa ccggacttcc tttaaaatca
     5581 gatccggatt catccgtgcc tgttcactgt cttcctgaaa gagctttatg cgccattccc
     5641 cgttgtcctt tgcgtagtca agccttaaag ggccaccact gaaagtataa tgttcccgta
     5701 aagtccaagg gagtttttgt gcaggcgcct gcgtcgttgc cttagcaggt gcttttgctg
     5761 aggctttggc tggtgctgcc agaacaactg tatgggacag ggtaaaggtc acagccattg
     5821 cgcatagaaa tcgaagagcg aagagcataa aacattcctt tatttttaaa gagggggaag
     5881 gctttccatt gcaggctcag gtaaaccccc gttattgttc tgaagccgcc agtagtatac
     5941 taaaacctgc cttaaggggc aactgttggc tttatactgt taacctttaa gcaatattta
     6001 tcgtataata ataaagtaaa ctacagaaat tatctgcata gtcaatcaac accgccggaa
     6061 ggcgatcagg acaactgcgt tttttttgga gcgcatctgc tacagtacgt ctcatattta
     6121 tttcaaatag ataacacggt acaacggata aacactccct gcttgtatcg ttcactcact
     6181 ttgaagtaat catgtgtttt ttgatatact gttacgtcgt tgcgccactg aaaaggcatc
     6241 ggttcatgaa caacttaaca attccaattt cctgtattgg cggcgacggc tacgagttcg
     6301 aggagtgcat ccctgtagaa tctattcagc cgcctgatac gccggcggtg actgtaggtt
     6361 cggtgtgcat atcgggcaga tttacaaaat ttcgtgaatc atataccttt cgtggcagag
     6421 tgacgggcgc atatcacgga acctgttgcc aatgccttgc ctcggcagac gtcccctttt
     6481 gtgtacaagt ccgatggatt tttgaaaaaa tatccggaaa tgtgctgaag gaaatcagcc
     6541 gttcgcagga agagacagac ttcttttaca gtcatccggc gaaaaacgct tcagcaccgg
     6601 cagcgcagga agatgtgatt tgcctggctc cttacgtatg ggaggaggtt gtttttgcag
     6661 agccgtatac ctatgtatgc ggtccggact gcaaaggtgt ctgtggccga tgcggcacca
     6721 acttaaatca taatgactgt ggctgtgaca aggaaaaaga gtcacagcac ctgaatgaca
     6781 atcagggatt gtcggcgctg gcaaagatgt ttccggagct gcaatccgac aagaataagg
     6841 agtagtttac aatggcatta cccaagagac gtaccggcaa agcgaaaaaa ggcatgcgtc
     6901 gctcacacca tgcacttaca gccccaaatg tagttgaatg ccccgattcc ggcgaagcca
     6961 agcttcctca tcgtgtttgc cttaaaactg gattttataa aggccgcgtt gttgtaaagc
     7021 ctcagaacga ctgagttgtt ttcgccggca tttttttagc accggtttgc ctgatctgaa
     7081 ctgagataaa ggatttctct gtgcgaattg ctttggatgc catgggaaca gataatccgc
     7141 ccggaccgga ggtggaaggt gctgtattgg caagcctcaa ttcagacgtg gaggtcatct
     7201 tggtcggtga tgaagatctg ctgaaagaga aactcgaagc ttttccgaaa aaaggcaacg
     7261 tctccatccg ccacgctcct gaggcaataa cgcccctcga gccgccggtt attgctgttc
     7321 gcaaaaaaaa gcattcctcc atgcatgtgg cgatgcgcat ggtcaaagaa ggggaggcac
     7381 aggcatttat cagcgctggc aataccggtg caattatggt ggccacccgt gtgattctgg
     7441 gatctattcc gggcgtggca cgccccgctt tgagtcagac cttccccacg ctgcagggac
     7501 gcacagttgt gcttgatctg ggtgcgaacg tggattgcac agcccggcat ttgtgtgaat
     7561 ttgccgagat gggtgtcgct tattcaagac atgccctggg cgtcgagaac cccaggattg
     7621 ccctgttgaa tatcggcgag gaaaacgtca agggcggtgc gattgcccgg gaggcatacc
     7681 gaacgctttc aaaagcgtcc cacctgaatt tcataggcaa tattgagccg agaccgcttt
     7741 gcgagggcaa ggcggatgtg gtcgtctgcg atggctttat cggcaacctc tttctaaaaa
     7801 cgagtgaggc cgttgcctat ttcatgggaa aaatggtgag ggagcatttc gaaagctcaa
     7861 atatgagcaa gatcggcgct gtcctcgccc gcaaggctct caagcagatc aagcacaaaa
     7921 tggatcccaa cgagtattcc ggtgcgccgc tgttgggcgt tgacggaaca gtgattgttc
     7981 ttcacggtgc cagcactgtt cggggcatag aaaatgcgat ctacggaaca cagcatgctc
     8041 tggaaaacca cctgctggaa catatccgcg agaacatcca gacattaaaa gaaaatggtg
     8101 gagtaaacgg atctgctcag gatgctttgt cagccgattc catggcaggg ctcgggctcc
     8161 agtccgaaac ggaagatcct ccggaagcag ccgtcttttc gtccgctgaa tcatcgaatc
     8221 tgcttcagta atctcttctc ttgcttttct tctttccttt cctgcacgat aaagcaggca
     8281 cgtttgtgag agaaaccagg cttaaaatgt gatccaagga aagcgcattg tcatcattaa
     8341 agggaaaaat cgctgtaatc acgggaggca cccggggaat cggatatgcg gttgcccggc
     8401 tttttattga aagaggggcg caggttgctt tgtgtggccg ttccgaggaa agtgcatcgg
     8461 ctgccgctca gcgcctcggt tcttcctgca tcggtatagg ctgcgatgta tccaatccgg
     8521 cagcagtgga cgcgctcatc ggcagagtcg ccgccacgtg gggaacggtt catattctag
     8581 tgaataatgc aggatcgacc gatgacggcc ttctgatccg gatgaaagat gagcgtctgg
     8641 agcggctcat acaaaccaat ctcagcagtg tgttttatac ctgccgtgcc acagctgcca
     8701 tgatgatgaa acagcgttac ggccgtatca tcaatatcgg ctccgttgca gggctgcgtg
     8761 gcaatgccgg gcaatgtgcc tacgcggcgg caaaagcggg gctgatcggc tttaccaaat
     8821 cctatgctcg ggaaatggct tcccgaaata ttactgttaa tctggttgcc cccggattta
     8881 ttgacacgga aatgacagcc tcctttccgg aggcgcagcg tgatgcgctg ctctcccggg
     8941 ttccgctgca gcgcccggga actgcggagg aagtggtggc agcagtggct tttcttgctt
     9001 cggatgaagc ggcgtatatt accggagcca cgcttaccgt ggacggcggg ctgggcacct
     9061 gatccggaag gatatcagag caggcacttc agggttgcct gaagcgtgtt ttcgtaatta
     9121 cccgtggagt gcccgcattc aagcgctata aagtccgtgt agccgctttc tctgatagcc
     9181 ctgaatatat tggtgtagtt gagttcgcct gtgcccggct cattgcgacc tgggttgtcg
     9241 gcaacgtgaa aatgcccgat atgggcaata ttttccctga tattgcgtat tacattacct
     9301 tctgtaattt gctgatgata aatatcgaag agcattttga cgttgggact tccgaccgcc
     9361 tgtaaaatct caatggtctg atcggtgcgg gtcatgaaaa atcccttgtg atccaccaga
     9421 ggattcagag cttccattac aagaatcatg ccactgtcct caaggagggg cgcaagccgc
     9481 ttcaggcagg taatgactga ttccgtctgt gcttccgctg atgtaccttt caggctgttt
     9541 cctgttaatc ctataagccg tctgcaattc aactgttttg ccattgcaat ccgttggcga
     9601 agcatttcga agactttatc atgatcttcg ggagcaacca tccagccctc ggcaatagcc
     9661 ccggtacctg ccatacagct tatttccaga cccaaagcac aggccttttc ccggattttg
     9721 tcggcgtcac cgatgggcca aagccattcg tatgcggcaa gtccccagcg tgccacatgt
     9781 tccagtttgt cttccgggcg caaacctggg aaccattcca gaggtgcgga aaaacgcatc
     9841 cctgaatcgg aaaaggtcgg gttgttccca acgtcagaaa aagtggcagg gcaaagagac
     9901 acgcttgcag cggcagaagc agcagactgc aggaaacagc gtcggttcat acgtggactc
     9961 cttgtgggta tgccataagc cggcagacac tgcccgggct gtacgcaact agagagaggc
    10021 gcagtgattc cggagcacct cgatgatttg atcctgttgc tccgttgtca gctccggaaa
    10081 cacaggcaga gcgagcactt ctgtgcaggc tctttccgct tccgggcagg aagccttcgc
    10141 tgaaacatat gcctgaaaac aaggctgtcg atggagcggc agcggataga aaacgccgct
    10201 gcctatttcc cggtcagcca gcagctgccg ggcacggtcg cgctgcggta tgcgaattac
    10261 atactgatga tagacatgat acatgccatc ctgttccacg ggcgggataa tgccgggtac
    10321 atccttcagg cattgcgtat aataagcggc tttttcacgg cgcttttcgt tccactgtgc
    10381 cagatatttc agttttactg ccagtacagc cgcctgaatt gtatgtaaat ggctgttggt
    10441 tcccaccagc gcatgctgat agagggcgct tgccccgtgg cagcgcagca tacgcagttt
    10501 ttccgccagc gcatcatcat tggtaaggat ggcacctcct tctccggcgg cgcccagatt
    10561 tttggtggga taaaaactta acgctgcagc gattccccag tttccgggct gccggttatt
    10621 catgcgagta cccagtgctt gcgccgcatc ttcgataatg ggaagattgt actccgtggc
    10681 gatgccggtt aaagcgtcca tatcggcaca ctgtccgtac agatggacgg gcaggatgca
    10741 gcgcgttttc tgagtaatta attggcggac cgactgcgga tccaccgtaa aagtgtcggg
    10801 aagaatatcg gcaaatacag gaataccgcc tgcatttacg atagcgccgg ctgtagcgaa
    10861 aaaggtgaaa ggcgtcgtga tgacttcgtc gcccggatga agatccagag tttttaaca
//
LOCUS       DAOPWB010000104        10867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6123_length_10867_cov_4.961617, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000104 DAOPWB010000000
VERSION     DAOPWB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6123_length_10867_cov_4.961617"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1057
                     /locus_tag="PKN92_09615"
     CDS             95..1057
                     /locus_tag="PKN92_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HNZ49091.1"
                     /translation="MEASRGCPHRCAFCLSGAFPCLRYFPLHAFLHSMSRLLDRGARR
                     FKFIDRTFNVDLNRMHTILRFFRERPEIEQLHLHFEIMPDHLPPDTLALLASFPHDAL
                     HLETGVQSVNPDIQKAIRRYQRIEPSLENLHWLRTHTGCQLHADLIFGLPGESIDSFA
                     EGFNRLVALDVQELQLGLLKNLPGTPLGALSSPDTVYDTNPPYELLQNEHYSFDALQR
                     MKRFAKYFDLLHNHGNFPESLALLRQLPPSPFHVFEALTEQIWEQEGKTYGIPLVKMA
                     EHLHIFLCACLPDRKSTVSAVIEKDFRRFKGRRDRLPFLTGNSA"
     gene            1073..2251
                     /locus_tag="PKN92_09620"
     CDS             1073..2251
                     /locus_tag="PKN92_09620"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNZ49092.1"
                     /translation="MDQIKDDIEHLATARAHRRFGSSGEKQAADYILGRLKPLVDRAV
                     VMFFKIPLFSSLFPFVFLGEFTVIAALAFWWPAISFIYGLIVFSLYQAESFGFPLVSR
                     YFPGTSSCAVFGDRKHADFKTLLVFTAYLDSCDCWTQRPFFLRISRYFLLLIQFAMVL
                     LLAACALDAFASLHHATYPFTRFFYSAALVFFPLVMTAMLAGGYFNRDTVGANHNASG
                     MAALLHIAEKFREKELHGAALLFFFPGAHHGSRRVMQEVLPTLKVCYDRVLMINLEGV
                     GAGELCYTDSEGVLQQLPCSRDLVEAAVRCAPPYQARKVHLSSRITNAHLPLLQGFNA
                     ISILGLDQNNCPVNYAAQQDIVDNISVERVRAAADLAEALARDVIAATTDPMNACAEP
                     "
     gene            2289..3194
                     /gene="thiF"
                     /locus_tag="PKN92_09625"
     CDS             2289..3194
                     /gene="thiF"
                     /locus_tag="PKN92_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HNZ49093.1"
                     /translation="MIPSLTITVNEHSRQITRGTSLYALRDALYPDADVLVVNGHPAD
                     SDRHLEEGDAVVLIRRGAFPAPEELESLMAARHTPGVHAILRQACVGIAGLGGLGSSI
                     AIALARSGIGSLILADYDVVEPSNLNRQQYFVEQLGMLKTEALHETLKRIHPSIRCTL
                     HAVKLTKDNTAALFKEVHVLAEAFDKAAEKAMLVEAALSADPPIPVVTGSGMAGYAPS
                     NLIVTRQLSRTLICCGDGITDARPGQGLMAPRVGIVAHHQANAILRFLLGLRCDVDTQ
                     EVQTSHGTIRTDIINNPTDIRNDTE"
     gene            3191..3859
                     /locus_tag="PKN92_09630"
     CDS             3191..3859
                     /locus_tag="PKN92_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49094.1"
                     /translation="MIRKLLLTLPLLLVLFGCSDFLKKTPPPPAQETAGPKNKEEAQA
                     LIRPAIEPLRKTMQPGGPGISEAERQQVLLALQHAIVTYGDNQYGKEVLRDLGYELQD
                     LARQASAQERYRLVLICIEASNLLEVNSAYLKRAGAQATTMLQKPMVSVKGFMDDLET
                     KQLTVFLELTDYFTGKIDRVQAREGDEFNNLRLVRVIGRNKSVLFEYLKVPGLFFEVQ
                     SFAP"
     gene            complement(3841..5130)
                     /locus_tag="PKN92_09635"
     CDS             complement(3841..5130)
                     /locus_tag="PKN92_09635"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49095.1"
                     /translation="MNLISRRQCIHYGSCGGCAAQDVPYEMQLAEKQSALGALFRNDW
                     QEPIPVTPSPVLRHYRNKIDPAFSFKWYEEAPPRDFLRETVLGFKVKGNWRRPLELEE
                     CLIAPEGVDALFPAVRDWVRVSGLKAYHSRKNEGYLRHLLIRDGKRTGERMVMLITSP
                     GALPDPSGFVDAVQAVFPCASIYHGTFSGRADIAVAEESVLLFGKKYIEEVLSILPED
                     ACTPEAPVYDAPGSFLQAEQEKKATLHFQISPMSFFQTNPLAAERLYGFIRGWCRKYP
                     AGLLYDLYGGMGSIALQVSDLYPQIISVEEVAAASEDGRQNAGRNQVHHIEFVTASVQ
                     DFFKKHSSSGDLSPDARVILDPPRSGLHPKALRGLLEWAPSHLLYVSCNPARLQAEME
                     MLRSVYTLESLHAFDLFPHTPHVETVASFVRTQGAKD"
     gene            5129..6346
                     /locus_tag="PKN92_09640"
     CDS             5129..6346
                     /locus_tag="PKN92_09640"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNZ49096.1"
                     /translation="MSDILLFCRIGESVLADSVSSTDPPRKKQYILPVRPVQYKAEKL
                     RLVLQIYFFFPFFKIYNYRIYKPFSIKYGCLSMHTTFSSPDGQRIASIDHFRGYVIFG
                     MILVNYLGLFHCMPELLKHHDRSMSYADTIAPAFVFISGMSCLMTVQKKMKAGRKWLI
                     PALAKRFTILILIGIVLYNPSPRNWNWWWDALVDIGFAGLLSLLFLRCRQSVRCLAAC
                     AFLLTYQIFFSRCGYEPWVMSRSINGGPAGILPWGSLFLLGTLAWDLMNTRSFRKSMM
                     LFLVWGLGLCLTGCIFYFSWGDTKELWYLSQRGMTLPYTLISGGLCFLTFLPFRLLND
                     YGEKEIRPLRTFGINPLALYMIQYALLELYGTLFVSENAGILPALSGLAAFALVCYAA
                     AEKLRKDNLILRI"
     gene            complement(6355..7809)
                     /gene="hisS"
                     /locus_tag="PKN92_09645"
     CDS             complement(6355..7809)
                     /gene="hisS"
                     /locus_tag="PKN92_09645"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNZ49097.1"
                     /translation="MKAKLVKPQNLKGFQDQLPEDMLVRRRVIETIQKSYELFGFLPL
                     DTPVMESFETLMGAGGTEIAKEIFRLQTPEEEDAALRFDLTVPFARVISQYRDRLKLP
                     FRRYHMGPVFRADKPGPGRFRQFTQFDIDIAGARTIAADAEVVAVLCHAMNALGLTKE
                     GAPLFRIRISNRVLLDALLDGCGIEDPEKVKHILRVIDKLQKVGIDNVRKELGEGRID
                     DSGDPIPGVHLSEDTIEIITRFVEVATDSRERMIEKLEALLPQTKLAEEALAQMRELH
                     AFLTALDVSEEEAVFDPSLTRGLDYYTGPVFEIEIPACREVGSVGGGGRYNRLCSRFL
                     SQDIPATGASIGLDRLLAALSSLGLVTTQKSNVQVCIATVGKVARDEVLKIASELRAA
                     GISTMPYLGNKKNMGDQLSNADSYDIPVAVIIGEDELQRGEVSVKNLIQGKEIRQDIK
                     DHDEYRQAGTAGQQTVARTEMVSVILRILSSVER"
     gene            complement(7900..10116)
                     /locus_tag="PKN92_09650"
     CDS             complement(7900..10116)
                     /locus_tag="PKN92_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ49098.1"
                     /translation="MITALLFCLSLSGAFDISLAPDQPMPFIYVDDPLIIEFSSDTDA
                     EVEGRLLFRAAADGAETECRLDKMLVRGGTEYWYAVHNAPENRGLFYLNAELTVNGEA
                     MQFERRYCRIDRPAALTSLPIYAHCGGEENTCPVVALRNVGINTLRVEANNAALPAIA
                     QRAAAEEVHLLLVVPASGMAETLESIKPVVQSSCEHILRFEVVYDPSKAESARAAELI
                     RETGCPAGISFVVNNAAAFAALLGQNSTLPLRHVSLAGDVWPAASEVRRIRQMAVLQG
                     LEGLNIHVICPAWHPKSQQSYAEFLQRFFEYKAEGAGSLGINAALIADDMGTQEMMAW
                     LNGLSLRCKAGTYVGTLAAKDGKSTMLFRTGASWLAVIFGSEKQSAGLALSVDGSLNL
                     ELSDAFGNPLPVPEIKEGKLPLNTGTMPLYLTGDGGVILGDVALQQAARLSSEFLAKK
                     EITDALPPEFSQLVKTIGAEPKGAGCRLRFMELLRYLPGLEEALGFRTLPRQVVVPAL
                     MLIVDLARSLAVIEEDRAELFVEPLPDIMSKTEELQSLYLTASSGSSRTRERSDWILS
                     EVRRLLEEAETLEKTGRKIEATAVAQIAYWRAQCLKPASQAETVLDTGPLEPLVLPGE
                     APAEAATVPVPVELPPVKPEEAVKEQAAPQAETPQKEEAAKADKKAEPEKKEEAKEEK
                     VERNAAGDIVYSVKKGDNPYLIAQKYGVSLDDLLKWNKLTKKSRLSIGQKLIIKKK"
     gene            complement(10118..10729)
                     /locus_tag="PKN92_09655"
     CDS             complement(10118..10729)
                     /locus_tag="PKN92_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ49099.1"
                     /translation="MELLTFPVTLFETNCYVIRHEGQALVIDPGEGTPALLKSLENYD
                     VTLIVNTHCHCDHSGGNAAVIQQTGAPLACHAADLPLLRSISEQGRMFGVPFPSSPDP
                     DRLLNHGDILSLGDLEFEVRHTPGHSPGHVVLIAPGIALVGDVLFAGSIGRTDLPGGS
                     MKELLHSIHTQLMTLPDETKVYSGHGPATTIGEERINNPFLVK"
BASE COUNT         2452 a         3126 c         2718 g         2571 t
ORIGIN      
        1 aaggaaaaga cggggtgcga ggcatcgata atcttgtctg gcagatccct atcccctcta
       61 tacggaacag gatattgccc atagagttat ttatgtggaa gcatcccggg gctgcccgca
      121 ccgctgcgca ttctgccttt ccggcgcctt cccctgtctc cgctatttcc cgcttcatgc
      181 ctttctgcat tccatgagca ggctgctgga tcggggcgca cggcgcttca aatttattga
      241 ccgcacgttt aacgtcgatc tcaaccggat gcataccata ctccgttttt tcagagagcg
      301 gccggagatt gaacagcttc accttcattt cgagattatg ccggatcatc tcccgccgga
      361 caccctcgcg ctgcttgctt cttttcctca cgatgccctg catctggaaa caggtgttca
      421 aagtgtcaat ccggatatac agaaagccat ccgccgctac cagcgcatag agccttcgct
      481 ggagaatctg cattggcttc ggacgcacac gggctgccag ctgcacgccg atctcatttt
      541 cggtctgccc ggagaaagca ttgattcttt cgccgaaggg ttcaaccggc tggttgctct
      601 tgacgtgcag gaactgcagc tgggactcct gaaaaatctg ccaggcacgc cgctgggcgc
      661 attgagcagc ccggacacag tctacgacac aaaccctcct tatgaactgc ttcagaatga
      721 gcattactcc tttgatgccc ttcagcgaat gaagcgtttc gcaaaatatt ttgacttgct
      781 tcataatcac ggcaacttcc ctgaaagtct ggcactgctc cggcagctgc ctccgtcccc
      841 ttttcatgtc tttgaagcac tgacagaaca gatatgggaa caggaaggga agacatacgg
      901 cattccgctt gttaaaatgg cggaacatct gcatattttt ttgtgtgcct gcctgccgga
      961 ccggaaatca acggtttccg ccgtgattga aaaggacttc agacggttca aaggtcgccg
     1021 tgaccgtctt ccttttctca ccggaaacag cgcttagaaa tgcttcgcca ccatggatca
     1081 gatcaaagac gatatcgaac acctcgcaac ggcacgggcg catcgccgct ttggcagctc
     1141 cggagaaaag caggcagccg attatatact gggcagactt aaaccgctcg ttgatcgtgc
     1201 ggtagtgatg tttttcaaaa tccccctctt ctcttccctg tttccctttg ttttcctcgg
     1261 agaatttacc gttatcgccg ctctggcatt ctggtggcct gccatctcct tcatttacgg
     1321 tcttatcgtt ttttctcttt atcaggcgga atctttcggg ttccctctgg tctcgcgcta
     1381 ctttccgggc acttcaagct gtgctgtttt cggcgacaga aagcacgccg atttcaaaac
     1441 actgcttgtc tttacagcct atctcgacag ctgcgactgc tggacacagc gtcccttttt
     1501 cctgcgtatt tcccggtatt ttcttctgct gattcagttt gccatggtgc tgcttcttgc
     1561 cgcctgcgcc ctggatgcct ttgcttctct gcatcacgcc acctatcctt ttacgcgttt
     1621 tttctattcc gcagctctag tatttttccc tctggttatg accgccatgc tggcgggcgg
     1681 atatttcaac cgggacaccg ttggtgccaa tcacaatgcc tccggtatgg cagcgctgct
     1741 tcatatcgcc gaaaaattcc gggaaaaaga gcttcacggt gccgcccttc ttttcttttt
     1801 tcccggagca catcacggaa gcagacgggt catgcaggaa gtgctgccca ctctgaaagt
     1861 ctgctatgat cgggtgctga tgattaatct cgaaggcgtg ggtgccggag aactatgtta
     1921 taccgactca gaaggtgttc tacagcagct gccctgttcc cgagatctgg tggaagcggc
     1981 tgtccgatgc gccccgccgt atcaggcaag aaaagtacat ctgtcctcca gaataaccaa
     2041 cgcccacctt ccgctgcttc aggggttcaa tgccatttcc attctggggc tggatcagaa
     2101 taactgcccc gtaaattatg cggcgcagca ggatatagtg gataatatca gtgttgagag
     2161 agtccgcgcc gccgctgatc tggcagaagc actggcaaga gatgttatcg cagcaacaac
     2221 cgacccgatg aacgcttgcg cggaacccta aaattctttt tcagtatcct ctacagcacc
     2281 aacagaccat gatcccttcc ctcacaatca cagtcaacga gcattcccgg cagataacca
     2341 ggggcacttc actctatgcc ttacgggatg cactttatcc cgatgcggac gtgctggttg
     2401 taaacgggca ccctgccgac agcgaccggc atctggaaga gggggacgcc gtggtgctga
     2461 tccggcgcgg tgcctttccg gcgcccgaag aacttgaaag tctcatggcg gcacgccaca
     2521 cccccggagt acacgccata ctacgacagg cctgtgtcgg cattgcaggg ctgggcgggc
     2581 tgggctcttc catcgcgatt gccctggcac gcagcggcat aggcagcctc attctggcgg
     2641 attatgatgt agtcgaaccg agcaatctga acagacagca atactttgtt gagcagctgg
     2701 gcatgctgaa aactgaggcg cttcacgaga cactgaaacg cattcatcca tccatccgct
     2761 gcacccttca tgcagtaaaa ctcacaaaag acaatactgc cgctcttttc aaagaagtgc
     2821 atgttctggc ggaagccttt gacaaagcag cagaaaaagc catgcttgtg gaagcggcac
     2881 tttccgccga cccgcctatt cctgtggtaa ccggttccgg catggcaggc tacgccccgt
     2941 ccaatcttat cgttacccgg cagctcagcc ggacgctgat ctgctgcggt gacggcatta
     3001 ccgatgccag accgggacag gggcttatgg cgccccgcgt cggcattgtg gcgcaccatc
     3061 aggcaaatgc catcctgcgt tttttgctgg ggctccggtg tgatgtggac actcaggaag
     3121 tgcagacaag ccacggcacc atcagaactg atataataaa caatcctaca gacatcagga
     3181 acgatacgga atgatcagaa aacttctgct tacacttccc cttctgctgg tacttttcgg
     3241 ctgttcggac tttttgaaaa aaactccgcc gccgcctgcc caggagacag caggcccgaa
     3301 aaacaaagaa gaggcacagg ctctgatcag accggcaatc gaacctttgc ggaagacgat
     3361 gcagccaggc ggtccgggga tatcggaagc ggagcggcaa caggtgttgc tggcgctgca
     3421 gcatgccatt gtgacctatg gcgacaatca gtacgggaaa gaagtactcc gcgatctggg
     3481 gtacgagctt caggacttgg cgcgacaggc atcggctcaa gagcggtatc gccttgtgct
     3541 gatctgtatc gaagcctcca acctgctgga agtgaacagt gcctatctga aacgcgccgg
     3601 ggctcaggct accaccatgc ttcagaagcc tatggtatcc gtcaagggtt ttatggatga
     3661 cctggaaaca aaacagctga ccgtcttcct cgagctgacc gattatttca cagggaaaat
     3721 cgaccgggtt caggcacggg aaggagatga attcaataac ctgcggctgg tacgggtcat
     3781 tggcagaaac aaaagcgtgc tctttgagta tctgaaagtg cccggcctct ttttcgaagt
     3841 tcagtctttt gcgccctgag tccgcacgaa agaagccact gtttccacat ggggcgtatg
     3901 cggaaacaga tcaaaggcat gcagcgattc cagcgtgtaa acgctgcgaa gcatttccat
     3961 ttccgcctgc agccgtgcag ggttacagga tacatacagc aggtgcgacg gagcccactc
     4021 cagcagtccc cggagggctt tgggatggag ccccgagcgg ggcggatcca gaatcacccg
     4081 ggcatcgggc gacagatccc cgctgctcga atgcttttta aaaaaatcct gcacggacgc
     4141 cgtaacaaat tcaatatggt gcacctgatt gcgcccggca ttttgtctgc cgtcctccga
     4201 cgccgccgcc acttcctcca cactgatgat ctgcgggtat aaatcggaaa cctgcagcgc
     4261 tatgctgccc attccgccgt aaaggtcata cagcagcccg gcaggatact tcctgcacca
     4321 gccgcgaata aatccataaa gccgctcagc ggcaagcgga ttggtctgaa aaaaactcat
     4381 gggcgaaatc tgaaaatgca gcgttgcctt cttttcctgt tccgcctgca gaaaagatcc
     4441 cggagcgtcg tagacaggtg cctcaggcgt acaggcatct tcaggaagga tgctgagcac
     4501 ttcttctata tattttttac cgaaaagaag caccgactct tcggcaacgg caatatccgc
     4561 ccttccggaa aaagtgccgt gataaatgct tgcacaggga aaaacagcct gcacagcatc
     4621 gacaaatccg gaaggatcgg gcagcgcccc gggagaggtg atcagcatga ccatccgctc
     4681 gcctgtgcgc tttccgtcac ggatcagcag gtgacgcaga tacccttcat tctttcgcga
     4741 atgataagcc ttcagaccgg acacacgcac ccagtcacgg acagcgggaa aaagagcatc
     4801 caccccctca ggcgcaatca gacattcttc cagctccagc ggacgccgcc agttcccctt
     4861 taccttgaag cccagaactg tttcacgcag aaaatcgcgg ggcggcgcct cctcatacca
     4921 tttaaatgaa aaggcgggat ctattttgtt ccgataatgc cggagcaccg gcgagggagt
     4981 gactgggatc ggttcctgcc agtcgttacg gaaaagtgca cccaatgccg actgtttttc
     5041 cgccagttgc atttcataag gaacatcctg tgctgcacag cctccgcagc ttccatagtg
     5101 gatgcattgt cttctggaaa tcagattcat gagtgatatt cttttgtttt gccgtattgg
     5161 tgaaagcgta ttggcggatt ctgtttcctc aacagatcct ccacgaaaga agcagtatat
     5221 cctgcctgta agacccgttc aatataaagc agaaaaactc cggctcgtgc tgcagattta
     5281 ttttttcttc cctttcttca agatatacaa ttacagaatc tataaaccct tttccatcaa
     5341 atacggctgt ttgtccatgc acactacttt ctcatcgccc gacgggcaac gcatcgcatc
     5401 tattgaccac ttccggggat atgtcatctt cggtatgatt ctcgtcaatt atctggggct
     5461 ctttcactgc atgcccgagc tgctgaaaca tcacgacaga agcatgagct atgcggacac
     5521 catcgctccg gctttcgtgt tcatctccgg aatgagctgc ctcatgacgg ttcaaaagaa
     5581 aatgaaagcc ggcagaaaat ggcttatccc cgcactggca aaacgtttca ccatcctgat
     5641 tctgatcggt attgtcctgt acaacccttc gccccggaac tggaactggt ggtgggatgc
     5701 gctggtggat atcggatttg ccgggttgct ttcgcttctc tttctccgct gcaggcagtc
     5761 cgtccgttgc ctggcagcct gcgcttttct cctgacctat cagatatttt tcagccggtg
     5821 cggctatgaa ccctgggtaa tgagcagaag cataaacggc ggtcctgcag gcatcctgcc
     5881 ctggggcagc ctttttctgc tgggcaccct cgcctgggat ctcatgaaca cccggtcttt
     5941 ccgaaaaagc atgatgttgt ttcttgtctg gggcctggga ctctgcctga caggctgtat
     6001 cttctatttt tcctggggcg acacgaaaga actctggtat ctgagccagc gcggcatgac
     6061 actgccctac acgctcatct ccggggggct gtgctttctc accttccttc ccttccgcct
     6121 cctcaacgat tatggagaaa aggaaatcag accgctacgc actttcggca tcaatcccct
     6181 tgccctctat atgattcaat atgcactgct cgaactctac ggcacacttt ttgtatctga
     6241 aaacgccggt atcctgccgg cattaagcgg cttagcggct ttcgcattgg tctgctatgc
     6301 tgctgcggaa aagctgcgga aagacaatct gattctccgc atataaggta ccgcttacct
     6361 ctccactgat gaaaggatac gaaggattac ggagaccatt tctgtccggg caacggtctg
     6421 ctgtccggca gttcccgcct gacgatattc gtcgtggtct ttgatgtcct gccggatttc
     6481 tttcccctga atcaggtttt tgacagacac ttcaccccgc tgcagttcat cctcgcctat
     6541 aattacagca acaggaatgt catagctgtc cgcattactc agctgatcac ccatgttttt
     6601 cttgttgccg agatagggca tcgtcgagat gccggcggca cgcagctccg aggcgatttt
     6661 cagcacttca tcccgtgcca cttttcccac tgttgcaatg catacctgca cattgctctt
     6721 ttgcgtggtc acgagcccca gactgctcag cgcggcaagc agcctgtcca acccgataga
     6781 ggcaccggtg gcgggaatat cctgcgaaag aaaacggctg cacaaacggt tatacctgcc
     6841 ccccccgccc acagaaccca cttcccggca cgccggtatt tcaatttcaa aaacaggacc
     6901 cgtgtaataa tcgagccccc gggtaaggct gggatcaaaa accgcctcct cttcggaaac
     6961 atccagcgcc gtcaggaagg catggagttc tctcatctgg gcaagagcct cttccgccag
     7021 ttttgtttgc ggaagcagcg cttccagttt ttcaatcatc cgctctctgg aatctgtcgc
     7081 cacttcgaca aagcgggtaa taatttcgat cgtgtcctca gacagatgca cgccgggaat
     7141 gggatcgccg gaatcgtcaa tgcgcccttc gcccagttct ttacgcacat tgtcgatccc
     7201 cactttctgc agtttgtcga tgacccgaag aatatgcttt actttttccg gatcttcaat
     7261 accgcagcca tcaagaagcg catccagaag cacccggttg ctgatacgga tccggaaaag
     7321 aggtgcccct tcttttgtga gtccgagggc attcatggca tggcagagca cagcaacaac
     7381 ttccgcatcg gcagcgatag ttctggctcc ggcaatatca atatcaaact gggtaaactg
     7441 gcggaaacgt cccggccccg gcttatccgc ccgaaacaca ggccccatat ggtaacgcct
     7501 gaaaggcagt ttcaacctgt cccgatactg actgatcacc cgcgcaaagg gcaccgtcag
     7561 atcaaagcgc agcgctgcat cctcctcttc gggcgtctgc agccgaaaaa tctctttggc
     7621 aatctctgtg ccgcccgcac ccataagcgt ctcgaaactt tccatcaccg gtgtatcgag
     7681 gggcagaaac ccgaaaagct catagctttt ctgtatggtt tcaatgaccc ggcgccgcac
     7741 cagcatatct tccgggagct gatcctgaaa tcctttaagg ttctggggtt ttaccagttt
     7801 tgctttcata aacacttccg cctttattgt atgtattttt ttcagtcggt aaggctggtc
     7861 tcagaaaaaa cggtgtcgct gcagacctgc tgcgtcagat tactttttct taatgatgag
     7921 tttctgtccg atgcttaatc tggatttttt cgtcagtttg ttccacttaa gcagatcgtc
     7981 gagactcaca ccgtactttt gcgctatcag gtagggatta tcgccttttt taaccgaata
     8041 gacaatatct ccggcagcgt tgcgctcaac cttctcttct ttcgcttctt ctttcttttc
     8101 cggctcagcc tttttgtccg ctttcgccgc ttcttctttt tggggcgtct ctgcctgcgg
     8161 tgcagcctgc tctttcacgg cttcttcagg cttgacaggc ggcagctcca ccggaacggg
     8221 tacagtcgcg gcttctgcag gcgcctcgcc gggaagtacc agcggttcga gaggcccggt
     8281 atcgagtacc gtttccgcct gtgacgccgg tttgagacat tgagcacgcc agtaggcgat
     8341 ctgggcgaca gcagttgctt caatttttct tccggtcttt tccagcgtct ccgcttcttc
     8401 cagaagcctt ctgacttcgg acagaatcca gtcgctgcgc tcacgggtgc gggatgatcc
     8461 gctggatgca gtcagataaa gagattgcaa ttcctctgtc ttgctcataa tatccggaag
     8521 gggttccaca aaaagctccg ccctgtcttc ttcgatcacc gccagcgacc gtgcaagatc
     8581 gacaatcagc ataagcgcag gcacaacaac ctgcctggga agcgtacgga acccgagcgc
     8641 ttcctccaga ccgggcaggt accggagcag ctccataaaa cgaaggcggc agccggcgcc
     8701 tttgggttcc gcaccgatgg tcttcaccag ttgagaaaat tcaggaggca gggcgtccgt
     8761 tatttctttt ttcgccagaa attccgacga caggcgggct gcctgctgca gcgcaacatc
     8821 gcccagaatc actccgccgt cgcccgtaag gtacagaggc atcgttcctg tattgagcgg
     8881 aagctttcct tcctttattt ccgggacagg cagaggattg ccgaaagcat cggagagctc
     8941 cagatttaaa gaaccatcca ccgacagcgc caggcctgca ctttgctttt ccgacccgaa
     9001 aattacagcg agccaggaag cgccggtacg gaaaagcatc gtgcttttcc catctttggc
     9061 tgccagtgtg cccacataag tacctgcctt gcatcgcagt gacagaccgt taagccatgc
     9121 catcatttcc tgcgtaccca tatcatcggc aataagagct gcattgatgc ccaggcttcc
     9181 ggcaccttcc gccttatact caaaaaaacg ctgcagaaat tccgcatagg actgctggga
     9241 tttcggatgc cacgcaggac agatcacatg aatgttcagc ccttccagcc cctgcagcac
     9301 agccatctga cggatgcgcc gcacctcgga cgcagcaggc cagacgtcgc ctgccagaga
     9361 tacatggcgc aggggcagcg tcgaattctg gccgagcagg gcagcaaaag cggctgcatt
     9421 attcacaaca aaagaaatcc cggcaggaca gccggtctcc cggatcagtt ccgcagctct
     9481 ggcactttca gcctttgacg gatcgtaaac cacttcgaag cggaggatgt gttcgcagct
     9541 gctctgcact accggtttga tgctctccag tgtttcagcc atgccggatg cgggaacaac
     9601 aagaagcaga tgcacctctt ccgcagcggc gcgctgcgca atagcaggca aagcggcgtt
     9661 attcgcttcc acccgcagcg tattgattcc gacattccgg agcgccacaa cagggcaggt
     9721 gttttcctca ccgccgcaat gcgcatagat aggcagggat gtcagtgcgg caggccggtc
     9781 tatacggcaa tagcgccgct caaactgcat cgcctcgccg ttcacggtga gttcggcatt
     9841 gagataaaaa aggccgcggt tttcaggtgc attgtgtacg gcataccagt attccgtgcc
     9901 gccgcgcacc agcattttat ccagccggca ttctgtttca gcgccatcgg ctgccgcacg
     9961 gaaaagaagc cgcccttcca cctccgcatc cgtatcagac gaaaactcaa taatcagcgg
    10021 atcatccaca tagataaagg gcataggctg atcgggcgca aggctgatgt caaaagcgcc
    10081 ggacagactc aggcagaaaa gcaatgctgt aatcatgtca tttcaccaga aaaggattgt
    10141 taatccgttc ttcgccaatg gttgttgccg gtccatgccc gctgtagact tttgtctcat
    10201 cgggaagcgt catcagttgc gtatgaatac tgtgcagcag ttctttcatg cttccgccgg
    10261 gcagatccgt cctgccgatg cttccggcaa aaagcacatc acccacaagc gcaatgcccg
    10321 gtgcgatcag caccacatgc cccggtgagt gcccgggcgt gtggcgcact tcaaactcga
    10381 gatcgcccag cgacagaata tcgccgtgat tgagcagcct gtccggatca ggcgaagacg
    10441 gaaagggcac accgaacata cgcccctgct cggaaatact ccgcagcagc ggcagatccg
    10501 ctgcgtggca ggcaagaggc gctcctgtct gctgaatcac ggcagcgttg ccgccgctgt
    10561 ggtcgcaatg acagtgggta ttgacaatga gggttacgtc ataattttcc aggcttttca
    10621 gcagcgcagg cgtgccttca ccggggtcta tgaccagggc ctgcccttca tgccggatta
    10681 catagcaatt tgtttcaaaa agagttaccg gaaaggttaa cagttccatt gagggaattc
    10741 tccatggcag agatacgcta tcatgctaca cacccgccgg aggcaagtca aacaaaaaag
    10801 gcaggtactt gaacgcgggt tcaaattcga aacgcaatta ttgagaaaca aaaaaggaga
    10861 cattgcg
//
LOCUS       DAOPWB010000105        10796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6179_length_10796_cov_4.908388, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000105 DAOPWB010000000
VERSION     DAOPWB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10796
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6179_length_10796_cov_4.908388"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKN92_09660"
     CDS             <1..190
                     /locus_tag="PKN92_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49100.1"
                     /translation="GMTGRSRNDGASKHGGGSGDDGRGRNHGARSKHGGECSDDGRGR
                     NDGQCENGAMDGLTLWTG"
     gene            448..2886
                     /gene="lon"
                     /locus_tag="PKN92_09665"
     CDS             448..2886
                     /gene="lon"
                     /locus_tag="PKN92_09665"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNZ49101.1"
                     /translation="MASTSKKARKEPNVKREQNEKYTLPLLPLKDAVIFPHMATPIFV
                     GRSASLAAVEMAASKRTPLFLVLQKDASVDNPKATDLHRVGVFAQIVNVAGLPDGGLK
                     VVVEGLARGKVISYNLEGLPYIVEVSGMPTPASQSVQLKALKQTVLHQFEEYLENSQK
                     MGMDILFTIRSMTDEDQFADTVSAYLPLSLEERQTLLETVPLLKRYKLLVSYLEREIE
                     LAQIERDARRRIRKQIERGQREHYLQEQLRLIQSELGMMDESGVDTLELENMIESANM
                     PAEIKKKAMRELRRYERLPPMSPDSAVLRGYLEWLCDMPWSKRTRDAINLKKARETLD
                     ADHFGLEKVKERILEFLAVRKLNRAGKGPILCLVGPPGVGKTSLGQSIAQAMGRKFVR
                     LSLGGIRDEAEIRGHRRTYVGALPGRLIQGIKDAGVKNPVMMLDEIDKLSADFRGDPS
                     SALLEALDPEQNKHFSDHFLEVGFDLSEVFFITTANNEYNIPLPLQDRMEIVRLPGYT
                     PEEKYEIARLYLQPRQINECGLNKVGLTITEEGMETLIQRYTREAGVRELDRSIASLC
                     RKIARKIVDGETKVITVLDSKEIVALLGPPPYGEIQPETLADIGVAIGMAWTYTGGEV
                     LRIESSVMQGKGALQLTGQLGDVMKESAAAAYTYIRTRAASLGVAAEFYKNTDVHVHI
                     PEGAIPKDGPSAGLAMIVSLLSALRQEAPAPAIAMTGEITLRGRVLAVGGIKEKVIAA
                     YRAGICHVVLPDENEKDFPDLPLEIREKVRFSLVSRVEEALPILFPGIDGTKKSGARR
                     TPLVKKASGKKAKG"
     gene            2931..3512
                     /gene="yihA"
                     /locus_tag="PKN92_09670"
     CDS             2931..3512
                     /gene="yihA"
                     /locus_tag="PKN92_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNZ49102.1"
                     /translation="MASINAHFICSALKAEQFPPGNRPEIAFVGRSNVGKSSLLNKLL
                     GRKDLAKTSSKPGKTQTINFFDVEGRWYFVDLPGYGYARVARSVKDEWSRRMLDYLRH
                     RDSLKIVAVLLDVRHDPTENDLHMLELLEESEKPTLIIATKVDKLKRSQRDRQLKEIR
                     GQLGLDQDGLIIPFSSETGEGVRQVWDIVRTML"
     gene            3533..4363
                     /locus_tag="PKN92_09675"
     CDS             3533..4363
                     /locus_tag="PKN92_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ49103.1"
                     /translation="MCRSFSFFLLLCIAVFQGALSWADAQAEARATDHAFVSDFDGSE
                     ERYVLLLPKPLASPDGAPLLVMLHGHGSDRWQCIRQDRDECRAVRDMAQRFGLVLLLP
                     DYRAATSWMGPAAEADLRQILDEWKERHHPEKVILAGGSMGGASALTFSALHPDLVQG
                     VLAMNGIANHVEYTGFQDAIAESFGGTKDTVPEEYIRRSAELQATSLRMPVALTAGGK
                     DTVTPSDSIVRLAEKLRGQNTDTLLLLSPEGGHDTNYADACEALGWLFEKTGLRTPAP
                     "
     gene            complement(4360..6630)
                     /locus_tag="PKN92_09680"
     CDS             complement(4360..6630)
                     /locus_tag="PKN92_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011585710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNZ49104.1"
                     /translation="MQSQYFSRIAGELKISVHQVQAISGLLAEGATIPFMARYRKEAT
                     GSLDEVALTTIRDTLGQLEELDKRREAILKSLDERDLLTDALKAAVLQAESLAVLEDV
                     YLPWRPKRRTRAAIAREKGLAPLADALLLQEPGDPAELAAAYISEEKGVATAEEALQG
                     ARDILAEKVSEDGSARALLRDFFLQEGQCVTRVLKDKEEEGAKFRDYFDWSEKAATVP
                     SHRFLAMLRAEKEGIVSFSITPDREEAIRRLRALFVTASVPASQEVLRAVEDGYVRLM
                     EPSLESETRKTLKEAADREAIRVFADNLKELLMASPLGRKRVLALDPGFRTGCKMVCL
                     DAQGKLLHDSVIYPHSGERAREESGRMLLALCHQYEIEAVAVGNGTAGRETEAFVREV
                     MPDATIPLVMVNESGASIYSASETARSEFPDKDVTVRGAVSIGRRLMDPLAELVKIDP
                     KSIGVGQYQHDVDQARLKQSLEDTVMSCVNSVGVDLNSASRELLMYVSGLGPQLSGNI
                     VAYRNEHGPFSSRAQLKKVPRLGPRAFEQSAGFLRIPEGKNPLDGSAVHPERYGLVDA
                     MAKDLGCTVKDLLADTTLRARIRPENYVSDTVGLPTLYDILEELAKPGRDPRKQFEIF
                     QFSGDVQAIGDLLPGMRLPGIVTNVTAFGAFVDVGVHQDGLVHISQLADHFVRDPNEV
                     VRVQQRVQVTVIEVDVPRKRISLSMKSDAVPSERTVPGDRQKRAGGGKKNSEQQRSSG
                     SISLDLKDKLSAHFKN"
     gene            6900..9641
                     /locus_tag="PKN92_09685"
     CDS             6900..9641
                     /locus_tag="PKN92_09685"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HNZ49105.1"
                     /translation="MNMRLFLRLFGLCACSAFFAAPSFPQIVVGELRCENLKDPLGID
                     CAVPRVSWRVEPGKRGLAQSAYQIGVFEVHVNGGHEPKLLWDSGKVESSDSHLIPLDQ
                     VKLSSGMECQWMVRIWDTQGEASEWSAPARFTVGPADRPEAGIQDWEGRWIGVDWIHE
                     HQGPLPLMRHAFDLAEKPVRAYAHVCALGYYELYLNGEKIGEEVLSPAVSDYSKRGLY
                     LTHDVTDHLQAGENCVGIWLGRGWSTGVLGLLTDRGPVVKAQLELVFADGTRKKVVTD
                     ASWKTAKSHITPIGKGMSGGYGGEHVDAVKEITGWSRAGFDASGWQDVTVYDFDGEKG
                     PRIEAQMMEPNRLLDEITPASVKRHGDGYLVDMGRNYTGWFMLRFPDELKSGTEVHME
                     FADKQFPDGRFQTYSQQDTYVAKGGGGESFCNRFNYHAFRYAILKGLPREPKLEEISG
                     RMISTGYDHAAEFSCDNSLLNDIYNTTIWTHQCLSLGGYTVDCPHRERLGYGGDSGTS
                     MEAAMLNFKSNPFYAKWAEDWRLSQAPDGDVPYTAPNSQEAGGGPVWSGFCITMPWMV
                     YTSYGDLRILEKNWPMMKGWLKFIDTKMEDELLRPYVGIGNSLATWSFLGDWVPPGRK
                     QGKDRVDDLSTLFFNNCYLVHCLQLASKVAVLMGEPETAKEYADKAAHLAKTLHERFL
                     NEDGVTYANGEQTYLAMPLLFGITPPDKRAGVFSALEKDIVETRDGHLNTGMHGNYYM
                     ARLLLLERRNDLLTLMHTKETYPSYGFMLKNGATTIWEEWDGDNSQIHNTMISVGMWF
                     IAGLGGIQLDEAHPGFKHFTLAPGLESGLSMVRAVHHSPYGQILSAWKKDETGLEYLA
                     EVPPNSTASLVLPAASIDAVKESGLPLEESPGITNIRVQEGLLYCDLAAGNYHFQIG"
     gene            complement(9682..>10796)
                     /locus_tag="PKN92_09690"
     CDS             complement(9682..>10796)
                     /locus_tag="PKN92_09690"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNZ49106.1"
                     /translation="LSNCFNSHYRRILVLTQYKSNSLARHISHSWNIMHTELGEFIEV
                     IPPQMRVNSNWYLGTADALYQNLYSINQADPEEVLILSGDHIYKMNYQKMLRQHLETE
                     ADLTIGAIETPVSDASRFGVFEVDNSSRVLSFEEKPPQPRTIPGNPSAALVSMGIYIF
                     KAKVLKEVLEADAFLPGSSHDFGKDVIPRMVENQRAVYVFNFQDENKKDAKYWRDVGT
                     LDSYWEANMDLVSVDPHFNLYDRKWPMRTHVPSLPPAKFVFADPGVRFGAAVDSIVSP
                     GCIVSGSLVDHCVLGPEVRINSYSEVHESILFDRVVVGRKSRIRRAIIEKDVVVPEKT
                     VLGYNLQEDARYFRVTEGGIVVVESTEKLLAHQQKI"
BASE COUNT         2439 a         2910 c         3072 g         2375 t
ORIGIN      
        1 agggatgaca gggaggagca gaaatgacgg ggcgagcaag catggcgggg gaagcggtga
       61 tgacgggaga ggaagaaatc acggggcgag gagcaagcat ggcggggagt gcagtgatga
      121 cgggagagga agaaatgacg ggcagtgcga aaatggcgcc atggacgggt tgactctgtg
      181 gacgggttga ctccgtggac aaggtgactc tgtcgacgta gtgacacggg gtgcggagtg
      241 gtgcggtgca gtgtttcacc attgaatccg ttcgattttc agccgtcgtt atgagtcgtg
      301 tcattcaatt tcgtatacta taggcagggg gagcgctttt gtccgccgtt ttttatctgt
      361 tttatgtccg gctgtgtgcg ggcttttgcg tcgggaggga tcgcatcgca ttcaatcaca
      421 gcagcatctg atgcaggaac aagtcctatg gcttccacgt ccaaaaaagc acgaaaagaa
      481 cctaacgtaa aaagagaaca gaacgaaaag tacacccttc cgcttcttcc tctgaaggat
      541 gcggtgatct ttccgcatat ggcgacacct atttttgtgg gacgctccgc ctcacttgcc
      601 gccgtggaaa tggcagcatc taaacgcacc cctctctttc ttgtgcttca gaaggatgca
      661 tccgtcgaca atccaaaggc aacggatctt catcgcgtcg gtgtttttgc gcagattgtg
      721 aatgtggcgg gactgcctga cggcggtctg aaagtggtgg tggaaggact cgcccggggc
      781 aaggttattt cctacaatct ggagggcctt ccctatattg ttgaagtgag cggcatgccg
      841 actcctgcct cgcagagcgt acagctgaaa gcattgaagc agacggttct ccatcagttt
      901 gaggaatatc tggagaacag ccagaaaatg ggcatggata ttctttttac catccgctca
      961 atgactgatg aagaccagtt tgcagatacg gtgagtgcct atcttccatt gagtctggaa
     1021 gaaaggcaaa cgcttctgga aacggtacct ctgcttaaaa gatacaagct gctggtcagt
     1081 tatctggaac gggagatcga actggcgcaa attgagcggg atgcacggcg ccggatccgc
     1141 aagcagatcg aacggggtca gcgagagcat tatctgcagg aacagctccg tctgattcag
     1201 tccgagctcg gcatgatgga cgagtcaggc gtagatacgc tggagctcga aaacatgatc
     1261 gagtcggcga atatgcctgc ggaaatcaag aaaaaggcca tgcgcgaact ccgccgttac
     1321 gagcgtctcc cacctatgag cccggacagc gccgtgcttc gcggctatct cgaatggctc
     1381 tgtgacatgc cctggagcaa acgaacccgt gatgccatca atctgaaaaa ggccagagaa
     1441 acgctggatg ccgatcattt cggcctggaa aaggtaaagg agcgcattct cgagtttctt
     1501 gcggtgcgca aactgaaccg tgcgggcaaa ggtccgatcc tctgtcttgt tggcccgcca
     1561 ggcgtgggca aaacatcgct cggtcagtcc atcgctcagg caatgggccg taaatttgta
     1621 cgtctctcac tgggcgggat tcgcgatgag gcagaaatcc gcggtcatcg ccgcacctat
     1681 gtgggtgccc ttccgggccg cctgattcag gggatcaaag acgcgggcgt aaaaaatccg
     1741 gtgatgatgc tggacgagat agataaactg agcgccgatt ttcggggcga cccatcctcg
     1801 gctcttctgg aagcgctcga tccggaacag aacaaacatt tcagcgatca ctttctggaa
     1861 gtgggcttcg atctttccga ggtctttttt attaccaccg ccaataatga atacaacatt
     1921 ccgctgcccc tgcaggatcg catggaaatt gtccgcctcc ccggctatac accggaagag
     1981 aaatatgaaa tcgcccggct gtacctgcag cccagacaga tcaacgaatg cggactgaat
     2041 aaagtgggac ttaccatcac ggaagaaggg atggagaccc tgatccagcg gtatacgaga
     2101 gaagcggggg tgcgtgaact ggatcggtcc attgcctcgc tctgccgcaa gatcgcccgg
     2161 aagattgtgg acggcgagac aaaagtgatt accgttctgg acagcaaaga aattgttgcg
     2221 ttgctgggtc ccccgcccta cggcgaaatc cagccggaaa ccctggcgga tatcggcgtt
     2281 gccatcggca tggcgtggac ctacacgggc ggtgaggtgc tgcgtatcga atcgagtgta
     2341 atgcagggca aaggcgcact gcagctcaca ggccagctgg gcgacgtaat gaaggaatcg
     2401 gcggcggcgg cctataccta tatccgcacc cgggcggcat cgctgggagt tgctgcagag
     2461 ttttacaaga acacggacgt tcatgtgcac atcccggaag gggcgatccc caaagacggt
     2521 ccctcggcag ggcttgccat gatcgtatct ctcctgtccg ctctcaggca ggaggcaccg
     2581 gcacccgcca tcgccatgac cggcgaaatc acgctgcgcg gcagggtgct ggcggtgggc
     2641 ggaatcaagg aaaaggtgat cgccgcatat cgtgccggca tctgtcatgt ggtgctgccg
     2701 gacgaaaacg aaaaggactt tcccgacctg cctctggaaa tcagagaaaa ggtccggttt
     2761 tctcttgttt cccgtgtgga agaggcactc cccattcttt ttccgggcat tgacggcacg
     2821 aaaaaaagcg gagcgcgccg caccccgctg gtgaagaagg cttccgggaa aaaagcgaaa
     2881 ggctgaatcc cgcccggcgg ctcgccgttg tgtgaaaaga gaggcacttt atggcatcga
     2941 ttaatgctca ttttatctgc agcgctctga aagcggagca gtttcctccg ggtaaccgtc
     3001 cggagatcgc ctttgtcggt cggtcgaatg tgggcaaatc gagccttctt aacaaactgc
     3061 tgggcaggaa ggatcttgcc aaaacgagca gcaaaccggg caaaacccag accattaatt
     3121 tttttgatgt ggaagggcgt tggtactttg tggatctgcc cggctacggc tatgcacggg
     3181 tagcacgatc tgtgaaggac gaatggagcc gtcgtatgct ggactacctt cgtcatcgtg
     3241 acagtctgaa aattgtggcg gtactgctgg atgtgcgcca tgatccgaca gaaaacgacc
     3301 ttcatatgct ggagctgctc gaggaaagcg agaagcccac gctgattatt gccaccaaag
     3361 tggacaaact gaagcgcagc cagcgggaca ggcagctgaa ggaaatccgg gggcagctgg
     3421 gtcttgatca ggacggactg atcattcctt tttccagcga aacaggagag ggcgtccgtc
     3481 aggtctggga tattgtccgg acgatgctct gattgaagaa ggagttcgct ctgtgtgtcg
     3541 cagtttctct ttttttctgt tgctttgcat cgctgttttt caaggggccc tgtcctgggc
     3601 tgatgcgcag gcagaagcac gggcaacgga tcacgccttt gtttctgatt tcgacggcag
     3661 cgaagaacgc tatgtgctgc tgcttccgaa acctcttgca tcgccggatg gagcacctct
     3721 gctggtaatg cttcacggac atggatccga ccggtggcag tgcatccgtc aggatcggga
     3781 cgaatgccgc gccgtccggg atatggcaca gcgttttggg ctggtgctgc ttctgccgga
     3841 ttaccgcgcc gccacgtcgt ggatgggccc tgctgccgaa gccgacctgc gccagatcct
     3901 cgatgaatgg aaggaacgcc atcatccgga gaaagttatt cttgccggcg gatccatggg
     3961 cggggcatca gcgctcactt tctccgccct tcatcccgat ctggttcagg gggtcctggc
     4021 aatgaacggc attgcaaacc acgtggaata cacgggcttt caggatgcca tagccgaatc
     4081 cttcggcgga accaaagata ccgtgccgga ggaatatatc cggcgcagcg ccgagctgca
     4141 ggcaacgtcc cttcggatgc ctgtggcgct gactgctggc ggaaaagaca ccgtcacccc
     4201 gtcggacagc attgtgcggc ttgcagaaaa actccgcggg cagaacacgg acacattgct
     4261 tctgctgagt cctgagggcg gccacgatac caactacgct gatgcctgcg aagcattggg
     4321 ctggctgttt gagaaaacag gtctccgtac ccctgcgcct taatttttga aatgtgccga
     4381 cagcttatct ttcaaatcca gcgagataga tccgctgctt cgctgctgct ctgaattctt
     4441 tttcccgccg cccgctcttt tctgccgatc acccggaacc gttctttccg acggaaccgc
     4501 atcacttttc atggacaggg aaatccgttt gcgcggcaca tccacttcga ttactgtgac
     4561 ctgaacccgc tgctgcacac gcaccacctc gttgggatca cgcacaaagt ggtcagccag
     4621 ctggctgata tgcaccagtc cgtcctgatg tacgcccaca tcgacaaagg caccgaaagc
     4681 cgtcacattg gtgacgatac cgggcagacg catgccgggc agcaaatcgc cgatagcttg
     4741 cacgtccccg gaaaactgga agatttcgaa ttgtttgcgc gggtctcgtc cgggctttgc
     4801 cagttcctca aggatatcgt acagcgtcgg aaggcccaca gtatcgctca catagttttc
     4861 cggacggata cgtgcccgaa gcgttgtgtc cgccagaaga tctttcaccg tacagccaag
     4921 atccttcgcc atggcgtcca caagcccata acgttccgga tgcaccgcac tgccgtccag
     4981 tgggttcttt ccttcgggaa tacggagaaa tcccgccgac tgctcgaaag cgcgggggcc
     5041 caaccggggt acttttttca gctgagcccg tgaggaaaag gggccgtgct cgttgcggta
     5101 tgccacaatg ttacccgaca gctgcggacc cagaccggat acatacatga gcagctcgcg
     5161 gcttgcgctg ttgagatcca cgcccacgct gttgacgcag ctcatgacgg tatcttccag
     5221 actctgcttc agccgtgcct gatcgacatc atgctgatac tgacccactc cgatggattt
     5281 gggatcgatc ttcaccagct ctgccagcgg atccataagc cgcctgccga tgctgactgc
     5341 accacggacg gtaacgtcct tatccggaaa ttcagagcga gccgtttccg atgccgaata
     5401 aatggacgcg cccgactcat ttaccatgac cagcgggata gttgcgtccg gcatcacttc
     5461 ccggacaaat gcttccgttt ccctgcctgc tgtgccgtta cccacggcga cggcttcgat
     5521 ttcatattga tgacacaggg cgagaagcat gcgaccggat tcctcccggg cacgctcgcc
     5581 gctgtgcgga taaataacgc tgtcgtggag cagtttgccc tgcgcatcaa ggcagaccat
     5641 cttgcagccg gtacggaagc ccggatccag agcgagcacc cgtttccttc ccagaggcga
     5701 tgccatgagc agttccttga gattatctgc aaatacccgg atggcctccc tatctgccgc
     5761 ctctttcaga gtctttctgg tctctgattc cagggatggc tccatgaggc ggacatatcc
     5821 gtcttcaacc gcccgcaaca cttcttgaga agccggcaca gaagccgtca caaaaagggc
     5881 gcggagacga cggatcgcct cctcccggtc gggggttatg gaaaaactta caatgccttc
     5941 cttttccgcc cgaagcatgg cgagaaaccg atgggaagga accgttgcgg ctttttccga
     6001 ccagtcgaaa tagtccctga actttgcccc ttcctcttct ttgtccttaa gcacgcgggt
     6061 gacgcactgc ccttcctgca ggaagaaatc acggagcagc gcacgggcag agccgtcttc
     6121 actcactttt tcggcaagaa tatcccgggc gccctgcagt gcctcttccg ctgtggcaac
     6181 acctttttct tcactgatat acgccgctgc gagttccgcc ggatcgcccg gctcctgcag
     6241 cagcaatgcg tccgccagag gtgccagtcc tttttcccgg gcaatggcgg cacgggtacg
     6301 ccgcttcggg cgccagggca gatacacatc ttccagcacc gccagactct ccgcctgtaa
     6361 tacggctgct ttcagggcat cggtaagcaa atcccgctcg tccagcgatt tcagaatagc
     6421 ttctcgccgt ttgtccagtt cttccagctg acccagggta tctcgaatgg ttgtgagtgc
     6481 cacttcatcc aatgaacctg tcgcttcttt gcggtaccgc gccataaagg gaatggtcgc
     6541 accttccgca agaagtccgg agatcgcctg cacctgatga acggaaattt ttaattcgcc
     6601 ggctatacgg gaaaaatact gggactgcat cgatataaaa gccttatggg gaatcgttga
     6661 aaggaaaaga gacaggggga tgtaaaccct tgcccggagt gtaccggacc gttgtatggg
     6721 aacccccgga ggatggacgg cacaaccgtc ctgcgtattg aaccagaatg ggcaatttaa
     6781 agtcaatgac cgcggatcaa tgactgcgga tttatctttt ccgtgccctt tctgttgaca
     6841 tctccccgca accattccta aaatagcagt gaatcaaaac caaccacgga ggcttcccta
     6901 tgaacatgcg tctctttctg cgtctcttcg ggctctgcgc ctgctcagca tttttcgctg
     6961 ccccttcttt tcctcagatt gtcgtaggcg agctgcgctg cgaaaatctc aaggatccgc
     7021 tgggcattga ttgcgctgtg ccacgggtgt cctggcgggt tgagccgggt aagcgcgggc
     7081 ttgcgcaatc ggcgtatcag ataggcgtgt ttgaagtgca tgtgaatggc gggcatgaac
     7141 ccaagcttct gtgggactcg ggtaaagtgg agtcgtccga ctcccatctg atacctctgg
     7201 atcaggtgaa actgtcttca ggcatggaat gccagtggat ggtgcgtata tgggacacac
     7261 agggggaagc atcggagtgg agtgcgcccg cacgttttac cgtgggcccc gcagaccgtc
     7321 cggaagcggg cattcaggac tgggaagggc gttggatcgg cgttgactgg attcatgagc
     7381 atcagggacc gcttccgctt atgcgccatg cgtttgatct ggcggaaaag ccggtgcgtg
     7441 cctatgccca tgtatgcgcg ctcggttact atgagctgta cctgaacgga gaaaagatcg
     7501 gggaggaagt gctttccccg gcagtgagcg actactcaaa acgggggctt tatctgacgc
     7561 atgatgttac ggatcatctg caggctggag aaaactgcgt cggcatctgg ctggggcgcg
     7621 gctggtctac gggagtgctt ggtctgctga ctgatcgtgg acccgttgtc aaagcccagc
     7681 tggaactggt gtttgccgac ggcacccgga aaaaagtcgt gaccgacgca agttggaaga
     7741 cggcaaaaag ccatattacg ccgattggca aagggatgag cggcggttat ggcggcgagc
     7801 acgtggacgc agtgaaagaa attacgggat ggagccgcgc cggctttgat gcatccggct
     7861 ggcaggacgt gaccgtctat gattttgacg gagaaaaagg gccgcgcatc gaagcccaga
     7921 tgatggaacc caaccgtctg ctggatgaga ttacgcccgc ttctgtgaag cggcacggtg
     7981 acggctatct ggtggatatg ggtcgtaatt ataccggctg gtttatgctt cgctttccgg
     8041 atgagttaaa atccgggacg gaagtgcaca tggaatttgc ggacaagcag tttcctgacg
     8101 gcagatttca gacctacagc cagcaggata cctatgtggc aaaaggcggc ggcggcgagt
     8161 ccttctgcaa ccgttttaac tatcatgcct tccgctatgc cattttgaaa ggcctgcccc
     8221 gggaacccaa actggaagaa atcagcggac gcatgatctc gacgggctat gatcatgccg
     8281 cggaattttc ctgcgataat tccctcctca atgacattta caataccacg atctggacgc
     8341 atcaatgcct cagtctgggc ggctatacag tggattgccc gcatcgtgaa cgtctcggtt
     8401 acggcggtga ttccggcacc tccatggaag ctgccatgct gaatttcaag agcaaccctt
     8461 tttatgccaa gtgggctgaa gactggcgtc tgtcccaggc acctgacggt gacgtgcctt
     8521 atacggcgcc caattcccag gaagccgggg gcggtcccgt gtggagcggt ttctgcatca
     8581 ccatgccctg gatggtttat acctcctacg gcgaccttcg gattctggaa aagaactggc
     8641 ctatgatgaa gggctggctg aaattcattg acacaaaaat ggaggacgag ctgctcagac
     8701 cttatgtagg tatcggcaac agtctggcaa cctggagttt tctcggagac tgggtgccgc
     8761 cgggacgcaa acagggcaaa gaccgggtcg atgatctgtc aaccctcttc ttcaacaact
     8821 gctatctggt gcattgcctt cagctggcgt cgaaagtggc ggtacttatg ggagagccgg
     8881 agacggcgaa agaatatgcg gacaaagcgg ctcatctggc gaaaacgctg catgaacgtt
     8941 tcctgaatga agacggtgtc acttatgcca acggcgagca gacctatctt gccatgcccc
     9001 ttctttttgg cattacaccg ccggataaac gtgccggcgt cttttccgct ctcgagaagg
     9061 atatagtaga aacccgagac ggacacctga atacggggat gcacggcaac tattacatgg
     9121 cgcggctcct cctcctcgaa cggcgcaatg acctcctgac tctcatgcac accaaggaga
     9181 cctatcccag ctacggtttc atgcttaaaa acggtgcgac caccatctgg gaagaatggg
     9241 acggcgacaa ctcccagatt cacaacacca tgatttctgt cggcatgtgg tttatcgcag
     9301 gacttggcgg catccaactg gatgaagcac acccggggtt caagcacttt accctggcac
     9361 ccggtctgga gagcgggctg agcatggtgc gcgccgtaca ccattcaccc tatggccaga
     9421 ttctgagcgc ctggaaaaaa gatgagaccg ggctggagta tctggcggaa gtaccgccca
     9481 acagtaccgc ttcactggtg ctgcccgccg catccattga cgctgtaaaa gaaagcggtc
     9541 ttcccctgga agagagcccc ggtatcacca acatccgtgt acaggaggga ctgctgtact
     9601 gcgatctggc agccgggaac tatcattttc agatcggctg agcgatgaaa acagggcggt
     9661 tctttgtaaa agggccgccc tttatatttt ctgctgatgt gccagcagct tttctgtgga
     9721 ctcaaccaca acaatgccgc cttccgtcac ccggaagtag cgggcatctt cctgcagatt
     9781 atagccgagg acagttttct ccggaactac aacgtctttt tcgataatgg cacggcggat
     9841 gcggcttttt cttccgacca caacccggtc aaacaaaata ctctcatgca cttccgagta
     9901 ggagttaatc cgcacttcag gacccagaac gcagtgatcc accagactgc cgctcacgat
     9961 acagcccggg ctgactattg agtccacagc ggcaccaaaa cggactcccg gatcggcgaa
    10021 aacaaacttg gcggggggca ggctggggac atgggtgcgc atcggccact tccggtcata
    10081 cagattgaag tgggggtcca cgcttaccag atccatattt gcttcccagt aactgtcaag
    10141 tgtacccaca tcacgccagt attttgcatc ttttttgttt tcatcctgaa aattaaatac
    10201 ataaacggcg cgctggtttt ccaccatacg gggtataacg tctttgccga aatcgtggct
    10261 gcttcccgga agaaaagcgt cggcttccag tacctctttc agaaccttcg ctttaaaaat
    10321 atagataccc atggagacga gtgctgccga cgggttgccc gggatggtgc ggggctgcgg
    10381 cggcttttct tcaaagctga gcacccggct gctgttgtcc acttcaaaaa caccgaagcg
    10441 gcttgcatcg gacacaggcg tttctatggc gccaatggtc agatccgctt ccgtctccag
    10501 atgctgtcgg agcatcttct gataattcat tttatagatg tggtcaccgc tcaggatcag
    10561 cacttcttcc gggtcggcct ggttaatgga gtaaagattc tgataaaggg catcagcagt
    10621 cccaagatac cagttgctgt tgacacgcat ctgcggaggg atcacttcaa tgaactcacc
    10681 cagttccgta tgcatgatat tccagctatg cgaaatgtgc cgggcgagag aatttgattt
    10741 gtactgggtc agcaccagaa tacgccggta atgggaattg aagcagttgg aaaggg
//
LOCUS       DAOPWB010000106        10790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6185_length_10790_cov_5.613973, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000106 DAOPWB010000000
VERSION     DAOPWB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6185_length_10790_cov_5.613973"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="PKN92_09695"
     CDS             <1..1458
                     /locus_tag="PKN92_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49107.1"
                     /translation="GGMGGYGGGMMGGGMGGYGGGMGGGRGGYGGYGGGRDVTMISNI
                     SDLFTRIPDMMVGEAPAIPDMVGLSRIGTGATTGQLTSAGGYNAAQTGAALGSSIGAA
                     SSGLGGDVLTLLERLVPQVYEPYSEELLSDMIYNPANNMLIVKNTASNLEQFEKQLKQ
                     IDVTPKQVSIEAKFLTIRLEDLKKIGFKWDASLSDLNSRKRSISTLEDNAYYYYDING
                     DGKDEAIPFYTRPDGKPVISNTITDSTIAGLVNPVASAPSTFNIVGNILNNADGDKLS
                     LTFDYLDGLEESELLSAPRVTTMNRKPAIIADFATEYFVSSVDTQVYAFAGSGIGGNA
                     MTSFVQNIVPSAYNFGIALSVTPQIRDNNQVRLWLNPEVRTKTGTKTFEQKQVIADQT
                     ISNEINLPTTSWQAVWTNVIVHDGDTLVLGGLVQDKTSKSTHKMPYLADIPVIGFFFK
                     GSKRETNQSSLLIFVTPDIVDSTGARFFDVEDFEK"
     gene            1598..2863
                     /locus_tag="PKN92_09700"
     CDS             1598..2863
                     /locus_tag="PKN92_09700"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNZ49108.1"
                     /translation="MRYLIYNLLLYICAPLVFLYLLFSKRNRGMIRRFQAELHRCPES
                     PIWFHACSVGEVGVAALLIKELSAQLPDNAFLLSVSTRTGMKQAQASKTNAVLTYLPF
                     DLPHLIRRFVNHYRPSVLILIETELWPNLVKTMYLFGKPVVVVNARLSDKHFPRYKFL
                     NKVAPQVFSMLSCVCTQEETYNARFQALGVPAERICSTGNLKYDQEPLFLSPEQRCAV
                     KKEIGIGERAQVVIFGSTHPGEEKLAARCWEELSAQYEDLFLIIAPRHLNRMKSAVEP
                     FAGTDFRRRSLLGEPQGSRENSRILFLDTMGELAKLYQIADIAVIGGSFFPGVEGHNP
                     LEPAAVGVPVLFGSHMESFKDAVRLLLEAGGAVQVTSPEALSGALERLLEDKPLRAKM
                     GNNGLDAVKKNQGATGRTLAEVLSWLEKR"
     gene            complement(2917..5151)
                     /locus_tag="PKN92_09705"
     CDS             complement(2917..5151)
                     /locus_tag="PKN92_09705"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNZ49109.1"
                     /translation="MPNQRRVYFEALVCIVALFIVPAVLIAPTLKSGSLPFDLSELTL
                     RAPWQEAREGDFNSAQTETSSELVQRYFPWYAFLNESASHRRFPLWNPYEGCGIPFLA
                     LWRTRVFSIFSLPLYVFPLAAALAVSVFLKSAAAGLFAYFAARKFQFSPLLALVAGFG
                     YQLTGILLIGHWQPVSDVVPLLPLLLVAMQQLLLGEYRITALLALLLGITGLAGGPDG
                     FVAISLFLLLLIPVYRMRTREGEGYIAPVISLLLATGVSLGLAAVQILPYAEFLKFGT
                     LEDNYLPLIKLRDFAFILLSPRSVTDSIQANGAALWLPTCMIGFLLLPLWVSMRRFTV
                     RIRKRRVEALLITSLLFALLGMLLTAPLRKVHGLSFFDSHYFFLPLPLAFLFLTATTA
                     EEWLHLNLEHCKIVLRRMKYALLPFLALSFLVAWLLARHTIQAIPYFFPAAVFCVLLL
                     ILFIITLLKPRASWLVAGLCLLIAGNAWYSYRPGNHYTPPDQAIPDTLFVKSLCKENI
                     RISGTEKLAQWPLSIHGISQSYSPSGILLHRMKEFNQQASVNPELLRLTGAGQLVLTK
                     RDIQELFSRLRPVLNIQDVLPSGAVLLRDLNTHPLARISFAGRTLDPDKTAILRPSGP
                     PLVETDYIPATTSDTGKNARAQIASFASDEIVIRLEGSPTGILVVANSWYPGWHAQSN
                     GQEIPLFPADIAFQGVELLKGSQTLTLTYRPASFTLGLYISAAALFLVLVGMLYYVRS
                     IKRG"
     gene            complement(5320..6420)
                     /locus_tag="PKN92_09710"
     CDS             complement(5320..6420)
                     /locus_tag="PKN92_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNZ49110.1"
                     /translation="MKLKVKDLCSFMESWAPAGYAASWDRVGLHTGHPESEVHTVLVS
                     LSVTSETVQEAKRHGAQMIIAHHPLLFRPLESLREDRPHSRLCAEIIRSNIACFSAHT
                     NLDAAEGGVNDILAEKIGLQQTKPLLQDESQKQLKLITFVPESSVDALRGSLAEAGAG
                     TIGRYTHCSFQHSGIGTFFAGDGTDPVLGEKNTLNREPELRLEMILPYSKKTPVLKAL
                     LKSHPYEEPAYDLIALENPDPYMGLGRQGSLEEPVALSDFAKHVRKVLQLPHLICYGE
                     NDRTVQTVAVLGGSGGKLIAAIPAGIDVYVTGDIGYHDAELATARGLACVDAGHWGTE
                     LPVVETMKNKLKAAFPALSVISFLEKAPGTFM"
     gene            complement(6581..7390)
                     /gene="amrB"
                     /locus_tag="PKN92_09715"
     CDS             complement(6581..7390)
                     /gene="amrB"
                     /locus_tag="PKN92_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNZ49111.1"
                     /translation="MKMGNPNVAGLFYPADPEELQNFLVSALKQGTPAPPDSLPVALI
                     VPHAGYIYSGFTAGHAFARVQHCSPGRIVLLGVSHRYRFPGISLYSIPEVRSPIGTSP
                     VDIAFTRTLQQNFECVSPEAHEAEHALEVQIPFIQHLFKETPVIPVLFGMPPSQFHLD
                     FGKRLAQLLDEKDLVIASTDFSHYLPLPEAEKRDRQALSLIEQGDLPGLIHGLEKDDF
                     SLCGASAVVAALAFSETCGAEHRYILDYSTSGDWTQNYESVVGYGAVSLER"
     gene            complement(7422..10487)
                     /locus_tag="PKN92_09720"
     CDS             complement(7422..10487)
                     /locus_tag="PKN92_09720"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HNZ49112.1"
                     /translation="MNKLFLLCLGIISVPFSGWFCCGAHEIPPVSEDLPFIEPLFPEE
                     HLNGSFEMLDGSGVPQGWHLESPRGGVLQDSASNGKNSIWTEGTGSAEEMLAWFSPFF
                     SLKPGTQYLLEVDARRDSLGKGGGSAFVGTEKYNVDVFYLPEVWKTLRYGFCTPYQEQ
                     EYRYRVGQWQDDSRFHFDNIKLYRAMPVYARFGKVELGEGERIRGNDYFFSTTQVPGR
                     SNVYRFSREFTNHFNTDRLLFFDGSYAIFEHQVEEHLFDDLTLLFWNRCFGDSSLKVE
                     VSRSGQEWQELGILKEGARQTLTPPSDFFPLKTLWVRLSGITATESSLQILEYRVEAK
                     LAGRAMTAFGSTRWLKMAEPDADLDIEVDAPALQSPERFKEGITVRTFRNGAPLTGDV
                     VLKADGKEFSKAPLGAALAVPIETSLDGLALESDGIRLYYDSPFPEYFTATYGEQLDS
                     DLWWTSSGWKIRKNRPLPSARGAALRIETARNEAEAAQLVLLPEKRLTNVQVSVTDLA
                     GQDGSVIPTAAIDVLKVGYVPVLLPTDPTAPPADWADPLPPQKEGLSVEAGVNQPFWV
                     RVKPGKDTSAGEYRGKLLVTADGDYYREVPFEVHVFDFTLPDRMTCVTTLGLDHDLAI
                     RYHGVSTDADRRRVVDAYLKAMADHHLTPHDLAPLDPFRVHWPDISPENPPEDIKSLK
                     VYIDWDAYDRGTERGFNEFHFNSVRIPLQGMGWGNFHYTKEPSICGFTEDTPVYQHLF
                     ADYCRQLQAHFQEKGWLEDSFIYWFDEPDEKDYPFVLNGFKKFKKHAPGIGRMITEEI
                     AEGLYGGPDIWCPLPFLWNEKDARAREALGERIWWYVCTLPKEPYAGLFIDHPATDFR
                     VWLWQTWERKISGFLIWHTILWNSVTGYPDPDHPQNPYEDAMAWAYGYSENTPLPWGN
                     GDARFLYPPEAVANGRPSKPNLDAPVDTIRIEMLRDGIEDYEYLAMLKRGIENKRAYL
                     SAEEFKTLEALLEVPDAISRSLEVFTKDPAPIEARRRELAQALELLEKK"
     gene            10592..>10790
                     /locus_tag="PKN92_09725"
     CDS             10592..>10790
                     /locus_tag="PKN92_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49113.1"
                     /translation="MSAEMAVRARVSSGTGNSSPGNANLRIGTHADHDDDGHSDSMRK
                     YVPRHASSISPTHDAVIAPFRR"
BASE COUNT         2725 a         2736 c         2793 g         2536 t
ORIGIN      
        1 ggcggcatgg gcggctacgg cggtggcatg atgggcggcg gcatgggcgg ctacggcggt
       61 ggcatgggtg gcggacgcgg tggctatggt ggctatggtg gcggtcgtga cgttaccatg
      121 atcagtaata tttctgatct cttcacacgt atcccggata tgatggtggg tgaagcgcct
      181 gccattccgg atatggtcgg tctgtcccgt atcggaacag gtgccacaac agggcaactt
      241 acatctgcag gtggctacaa tgctgcacag acaggtgctg ccctcggcag ttcaatcggc
      301 gccgcttcct caggacttgg cggggacgtt ttaacactgc ttgaacgact ggtaccccag
      361 gtttatgagc cctactcgga agaacttctt tccgacatga tttataaccc tgccaataat
      421 atgcttatcg ttaaaaacac tgcgagcaat cttgagcagt tcgaaaagca gttgaaacag
      481 attgacgtta caccgaagca ggtaagtatt gaggccaaat tcctgaccat tcgtctggaa
      541 gacctcaaga aaatcggctt caagtgggat gcctccctat ctgacctcaa ttcgagaaaa
      601 cggtctattt ccacacttga ggacaacgcc tactattatt acgatataaa tggtgacggt
      661 aaggacgaag ccattccctt ctacacacgg cccgacggaa aaccggtcat ttccaatact
      721 attaccgatt cgaccattgc cggtcttgtc aatcctgtgg cttcggctcc atcgactttc
      781 aatattgtag gtaatattct gaacaatgcg gacggagaca aactaagtct taccttcgat
      841 tatcttgatg gtttggaaga gtctgagctg ttaagtgcgc cccgggtgac aaccatgaac
      901 cgcaagccag cgattattgc tgactttgct acagaatact tcgtttcatc ggttgatacg
      961 caggtctatg cctttgcggg ctcaggcatc ggcggaaatg ccatgacgtc ttttgtccag
     1021 aacattgttc cctcagccta caatttcggt attgctcttt ccgtgacgcc tcaaattcgt
     1081 gataacaacc aggttcgtct gtggctgaat cctgaagtgc gtacgaaaac cggaacaaaa
     1141 accttcgaac agaaacaggt gattgcagac cagaccattt caaatgaaat taatctgccg
     1201 accacttcct ggcaggcggt ctggacaaac gtgatcgtac acgacggtga cacgctggta
     1261 ctgggtggtc ttgtccagga caaaacctcc aagagcaccc ataagatgcc ttatctcgca
     1321 gacattcccg taattggctt cttctttaaa ggctccaagc gcgaaaccaa tcagtccagt
     1381 ctgcttattt ttgtgacacc tgatattgtt gattcaacag gtgcacgctt cttcgatgtc
     1441 gaagatttcg aaaaataagg tctggttctc aagtttgttc gcagggcggc tctttttaag
     1501 agtcgccctt tttattgctc agtaaaatag cggtaaaggc tttggttaag cgtccttagt
     1561 gcgcaacacg gtttatgagc ccatcgtgaa ggaaaacatg cgctacttaa tttacaatct
     1621 cctgctgtat atatgcgctc ctctggtttt tctttatctg cttttttcca agcgtaatcg
     1681 tggaatgatc cgacgttttc aggcggaact tcaccgctgc cccgagtctc cgatctggtt
     1741 tcacgcctgc agtgtaggtg aggtgggcgt agctgcgctt ttaattaagg aactgtcagc
     1801 acaactgccg gacaatgctt ttcttctatc tgtaagcacg cgcaccggta tgaaacaagc
     1861 acaagcttca aagacaaatg ccgttctgac ctatctgcct tttgatcttc cccatttaat
     1921 acgccgtttt gttaaccatt accgtccttc cgtcttgata cttattgaaa cggagttgtg
     1981 gcctaatctt gttaaaacga tgtatctttt tggaaagcct gttgtagttg taaatgccag
     2041 attaagtgac aaacactttc cacgatataa gtttctgaat aaagtggcac cgcaagtgtt
     2101 tagcatgttg agctgtgtat gtacacagga agagacctac aatgcccgct ttcaggctct
     2161 gggtgttcct gccgaacgca tttgcagcac tggaaatttg aagtatgatc aggagccgct
     2221 attcttgtca ccggagcagc gttgcgctgt gaaaaaagaa atcggtattg gagagcgggc
     2281 acaggttgtg attttcggaa gtacccatcc cggagaagaa aaactggctg cccgctgctg
     2341 ggaagaactg tctgcgcagt acgaggactt gtttttaatt attgcccccc gtcatttgaa
     2401 ccgtatgaaa agtgccgtgg agccttttgc cggtaccgat ttcagaagac ggtctctgct
     2461 gggtgaacca caggggtccc gtgaaaatag tcgcattttg tttctggaca ccatgggcga
     2521 actcgcaaaa ttataccaga ttgcggatat agccgttatt ggcggcagtt ttttcccggg
     2581 agttgaagga cataatccgc ttgaacctgc ggcggtgggt gttcctgtgc tttttggcag
     2641 tcatatggaa agtttcaaag atgcggtacg acttttgctg gaggctggcg gtgctgtgca
     2701 agttacgtcg ccggaggctc tttccggagc gttggagcgt ttattggaag acaagcctct
     2761 gagagcaaaa atgggcaata atgggctcga tgcggtaaaa aagaatcagg gcgccacagg
     2821 gagaacgtta gcggaagtgc tatcctggct ggaaaagaga taaggtaaca cttgtatcag
     2881 gcaaaggcgg ctgtatcaga aaaacccttg gccgggtcat cctcttttga tgctgcggac
     2941 gtaatagagc atcccgacaa gaaccagaaa aagagctgca gcacttatat aaagccccag
     3001 tgtaaaagag gcgggtctgt aggtgagcgt gagggtctgg ctccccttca gcagctctac
     3061 gccttgaaaa gcgatgtctg cagggaacag aggaatttcc tgtccgtttg actgggcatg
     3121 ccacccggga taccaggagt ttgcaacgac cagaatcccc gtaggagatc cttcaagacg
     3181 gataactatt tcgtccgagg caaaagacgc aatctgggcg cgcgcatttt ttcccgtgtc
     3241 cgaggtggta gcggggatgt aatcagtttc aaccagcggc gggccggaag ggcggagtat
     3301 cgctgttttg tcaggatcaa gcgtcctgcc tgcaaatgaa atccgcgcca gcggatgtgt
     3361 gtttaaatcc cggagaagca cagccccgga agggagaaca tcctgaatgt tcagaacggg
     3421 ccgtaatcgt gaaaagagct cctgaatatc tcttttcgtc aggacaagct gaccggcacc
     3481 ggtgagacgg agcagctccg gattaacact tgcctgctga ttgaactctt tcatgcggtg
     3541 caaaagaatg ccggagggcg agtagctctg agaaatccca tgaatcgaca gaggccattg
     3601 cgccagtttc tctgtaccgg atattcggat attctctttg cagaggcttt tcacgaaaag
     3661 agtatcggga atcgcctgat cgggcggcgt atagtggttg ccaggacgat aggaatacca
     3721 ggcgttaccg gcaatcagca ggcatagacc ggcgacaagc caggaagccc gtggcttgag
     3781 cagcgtgatt ataaaaagga tcaggagcag cacacagaat acagccgccg ggaaaaagta
     3841 aggaatcgcc tgtatcgtgt gccgtgcaag caaccaggcg acaagaaagg acaacgccag
     3901 aaaaggaagc agggcgtatt tcatgcggcg cagtacgatt ttgcagtgct ccaaatttaa
     3961 atgcagccat tcttcggcag tcgtggctgt taaaaacaaa aaagcaaggg gcaaaggcag
     4021 aaaaaagtaa tggctgtcaa aaaaagacag gccgtgtact ttccggagtg gggcggtaag
     4081 cagcatgcca agcagcgcaa atagaagcga ggtgatcaaa agagcttcta cccggcgttt
     4141 tctgatgcgc acggtaaagc gccgcatgga aacccacaac ggaagcagaa ggaagccgat
     4201 catgcaggtt ggcagccaca gtgccgcacc gtttgcctgg atgctgtctg tcacactgcg
     4261 tggcgaaagc agaataaacg caaaatctcg aagtttgatg aggggaagat aattgtcttc
     4321 caaagtgcca aattttaaaa attcagcata aggcagaatc tgaaccgctg caagacctag
     4381 ggaaacgcct gtggccagca gcaggcttat gacgggcgcg atatatccct ccccttcacg
     4441 ggtgcgcatt ctgtaaacag ggatcagaag gagcagaaaa agagatatcg caacaaagcc
     4501 atcgggaccg cccgccagcc ccgtaatgcc cagcaacaaa gccagcagag ccgtaatccg
     4561 gtattcgccg agaaggagct gctgcatggc tacaagcagc aggggcagca gaggcacaac
     4621 atctgaaaca ggctgccagt gtccaataag cagaatgccg gtaagctgat agccgaaccc
     4681 ggctaccaaa gctaaaagcg gagagaattg aaattttctg gctgcaaaat aggcgaaaag
     4741 acctgccgcg gcgcttttta aaaacacgga cactgccaaa gcggctgcca gcgggaaaac
     4801 atacagaggc agcgagaata tggaaaaaac gcgcgttcgc caaagagcaa gaaaagggat
     4861 gccgcagcct tcgtagggat tccacagggg aaaacgccgg tgcgatgcgc tttcatttaa
     4921 aaatgcgtac cagggaaaat aacgctggac aagctcgctc gaggtttctg tctgagcaga
     4981 attaaaatcg ccttcccgtg cttcctgcca gggggcacgc aaagtcagtt cgctgagatc
     5041 aaagggcagc gaaccgcttt ttaatgtggg cgcaataagt actgcaggca caataaaaag
     5101 cgcaacgatg caaaccaagg cttcaaaata gactcgtcgt tggttaggca caaaacagcc
     5161 ctttataaag ataatgcgaa cagatcattt cacatttccg cacccgtgcc gtggcggaat
     5221 gtctgctctt tttcaattaa atctgtaccc ctaagtatac cgtaaaacca gtaagggttg
     5281 catcccggcc gctcaaatga ccgcacgtga ccttccagct tacataaagg ttccaggcgc
     5341 tttttccaga aaagaaataa cgctaagtgc cggaaacgct gctttcagct tattcttcat
     5401 cgtttcaacg acaggcagct cggtacccca atgaccggca tccacacacg ccagccctct
     5461 tgctgtcgcc aattctgcat catgataccc gatatctcca gtaacgtaca cgtcaatgcc
     5521 tgcggggatc gcagcgatca gttttccccc gctgccaccg aggacggcaa cggtttgcac
     5581 tgttctgtcg ttttcaccat agcagataag gtgggggagc tgcagtactt tccggacgtg
     5641 cttagcaaaa tccgaaagag caacgggttc ctccaaagag ccctgcctgc ccagccccat
     5701 ataaggatct ggattctcca gtgcaatcaa atcgtaggcg ggctcttcat aaggatggct
     5761 tttcagcagc gcttttaaga caggggtctt tttcgaatag ggcagaatca tctccaacct
     5821 cagttccggt tcccgattca gggtgttttt ctcccctaag acagggtctg tcccgtctcc
     5881 ggcaaaaaaa gtgcctatgc cgctgtgctg aaaggaacag tgcgtatatc ggccgattgt
     5941 cccggcccct gcttccgcca gcgagccacg caaggcgtct acgctgcttt cagggacaaa
     6001 agtgatcagt ttgagctgct tctggctttc atcctgcagg agcggcttcg tctgttgcaa
     6061 tccgattttt tcagcaagta tatcgttgac accgccttcc gccgcatcaa gatttgtatg
     6121 ggcactgaaa caggcgatat tgcttctgat gatctctgca cacagccgtg agtgtggcct
     6181 gtcttctctg aggctctcca gcggtctgaa aagaagcgga tgatgcgcga tgatcatctg
     6241 tgcgccgtga cgttttgctt cctgcaccgt ctcagaggtc actgaaaggc taaccagcac
     6301 agtatgcact tctgattcag gatggcccgt gtgtaatccg acacgatccc aggacgcggc
     6361 atagcccgca ggcgcccagg actccataaa actgcaaaga tctttaactt ttaatttcat
     6421 gctccatttt ccttttctca aatgaaatgg cgatagaacc tgaatatcga aaggacggta
     6481 aatgattgat ggtcatggaa accctgagcg ggaaaaaggc atcaacataa ctattttatg
     6541 ccttctttct gcctgcgctg tttctgttta tcacacaatt ttatcgttcc agagaaacgg
     6601 caccataacc gacaacgctt tcatagtttt gcgtccagtc tcctgaggtg ctgtaatcca
     6661 gtatataccg gtgctcggca ccgcatgttt cggaaaaagc cagagcagca actacggcag
     6721 atgcaccgca gagagaaaag tcatcctttt ccagcccatg aatcaaaccc ggaagatctc
     6781 cctgctcaat aagcgacaat gcctggcgat cacgtttttc cgcttccggt aaaggcagat
     6841 aatgggagaa gtccgtggaa gcgattacca gatctttttc gtccagcaat tgagcaagcc
     6901 gcttcccgaa gtccagatga aattgggaag gcggcatccc aaaaagtaca gggatgaccg
     6961 gtgtttcttt gaaaagatgc tgtataaaag gaatctgcac ttcgagcgca tgctccgcct
     7021 catgcgcttc cggagaaaca cactcaaaat tctgctgtaa agtgcgggta aaagcaatat
     7081 caacaggcga ggtaccgatg gggcttctga cttccggaat gctgtaaagg gaaatgccgg
     7141 ggaagcgata gcgatgcgaa acgccaagaa gcacaatacg cccggggctg cagtgctgca
     7201 cccgtgcaaa agcgtgcccc gctgtaaatc cggaatagat atagccggca tggggcacaa
     7261 tgagagcaac gggcaaggaa tcgggtggtg ccggagtgcc ctgcttcaac gcactgacca
     7321 gaaaattctg caattcttcc ggatcagcag ggtaaaacaa gccggcaaca ttcggatttc
     7381 ccattttcat aggttacgcc ttttctgtct tttagatagc gctacttttt ttccaggagt
     7441 tcgagtgcct gtgccagttc acgccgccgg gcttcgatcg gagccggatc tttcgtaaaa
     7501 acttccagcg accgggaaat ggcgtcaggt acttccagca atgcttccaa ggttttgaat
     7561 tcttccgcgc tcagatatgc ccttttgttt tcaatgccgc gctttagcat ggcgaggtat
     7621 tcataatcct caatgccgtc gcgcagcatt tcgatgcgga ttgtatctac cggtgcatcc
     7681 agattaggtt tggagggcct gccattggca actgcttccg gcggatataa gaagcgcgca
     7741 tcgccgttgc cccagggcag cggggtgttt tcgctgtagc cgtatgccca cgccatggcg
     7801 tcttcataag gattctgcgg gtgatccgga tcaggatagc cggtaacgct gttccacaga
     7861 atcgtatgcc agatgaggaa gccggatatc ttccgctccc aggtctgcca cagccatacc
     7921 cgaaaatctg ttgccggatg gtcgatgaac aatccggcat agggctcttt gggcagtgtg
     7981 cacacatacc accagatcct ttcgcccagc gcttcgcgtg cccgggcgtc cttctcattc
     8041 cacaggaagg ggagaggaca ccagatatcg ggcccgccgt aaagcccttc agcgatctcc
     8101 tccgtgatca ttctgccgat gccgggcgca tgtttcttaa actttttaaa accattcagt
     8161 acaaaaggat aatctttttc gtccggctca tcaaaccagt aaataaagct gtcttcaagc
     8221 catccttttt cctgaaaatg agcctgcagt tgcctgcagt aatcagcgaa aaggtgctgg
     8281 taaacgggag tatcttctgt aaagccgcaa atggaaggct cctttgtgta atggaaattg
     8341 ccccaaccca tgccctgcag cggaatacgg acagagttga aatgaaattc gttgaagccc
     8401 cgttctgtgc ctctgtcata agcatcccag tcaatataca ctttcaggct ttttatgtct
     8461 tccggagggt tctccgggga gatgtccggc cagtgaacac gaaaaggatc cagaggggca
     8521 agatcatggg gcgtgagatg gtgatcggcc attgccttca aataggcatc gacgacccgt
     8581 cgcctgtccg catctgtgct gactccgtgg tagcggatgg ccaggtcatg atccagcccc
     8641 aacgtagtga cgcaggtcat tcgatccggc agggtaaagt cgaagacgtg tacttcaaaa
     8701 gggacttccc gataataatc accgtcggcg gtaacaagga gttttcctct gtattcgcct
     8761 gccgacgtat cttttccggg tttaactcgg acccagaaag gctgattgac tccggcttcc
     8821 accgatagtc cctctttttg tggaggcagc gggtctgccc agtcggcagg aggggctgtc
     8881 ggatcggtgg gcaggagaac aggcacataa cccactttca gcacatcgat cgctgcggtg
     8941 ggaattacgg acccgtcttg tcctgccaga tcggtgactg aaacctggac attcgttagt
     9001 cttttttcag gaagcagaac cagttgagcc gcctctgctt catttcgcgc tgtttcaatg
     9061 cgcaatgccg ccccccgggc ggaagggagc ggccggtttt tcctaatttt ccagcctgac
     9121 gatgtccacc agagatcgga gtccagctgt tcgccgtaag tggcagtaaa atattctgga
     9181 aaggggctgt cgtaataaag gcggatgccg tcactttcca gagccagccc gtcgagggac
     9241 gtttcaatgg gaacagccag agcagcgccc aaaggtgctt ttgaaaactc ttttccatcc
     9301 gctttcagaa ccacatctcc ggtcaacggg gctccattcc tgaaagtccg gacagtgata
     9361 ccttctttga aacgctccgg gctctgcaat gccggcgcat ccacctcaat atccagatct
     9421 gcgtccggct ccgccatttt cagccatcgc gtactgccga aagcagtcat cgccctgcca
     9481 gccagtttcg cttcaactct gtattcaaga atctgaaggc tgctttcggt ggcagttatg
     9541 ccggacagcc tgacccacag cgttttcaga gggaagaaat cggaaggagg cgtgagcgtc
     9601 tgccgcgcgc cttctttaag tatgcccagc tcctgccact cctgaccgga acgggaaact
     9661 tcaactttta acgaactgtc accaaaacag cggttccaga aaagcagcgt cagatcatcg
     9721 aaaagatgtt cttccacctg gtgttcaaaa atcgcataag agccgtcaaa aaagagaagc
     9781 cggtccgtat tgaaatgatt ggtaaattct cttgaaaatc ggtatacgtt gcttctgccc
     9841 ggcacctgtg tggtggaaaa aaagtagtcg ttgccgcgaa tacgttcacc ttctccaagt
     9901 tccacctttc cgaagcgtgc ataaacaggc atggcacggt acagtttaat attgtcaaaa
     9961 tgaaagcggc tgtcatcctg ccactggccc acacggtaac gatactcctg ttcctgatag
    10021 ggagtacaga agccgtagcg aagggttttc cacacttcag gcaggtagaa cacatcaaca
    10081 ttatattttt ccgtgccgac aaaggcgctg ccgccccctt ttcccaggct gtcccggcgg
    10141 gcatcaactt cgagaagata ctgtgtgccc ggcttgagcg aaaaaaacgg agagaaccat
    10201 gcaagcattt cttcagcgct gccggtgcct tctgtccaga tgctgttttt cccgttggat
    10261 gcgctgtcct gaagcacacc gccccggggg ctttccagat gccacccctg cggcacgccc
    10321 gagccgtcca gcatttcaaa agatccgttc aggtgctcct ccgggaacag cggttcgata
    10381 aatggcaggt cttcgcttac tggcggaatc tcatgagcac cacagcagaa ccatccggaa
    10441 aaaggaacac taatgatccc gagacaaagg aggaacaact tattcatagg aattccttgt
    10501 ttttgagtgc tggaaatgca gctgaatttg ccgttaaatt tgcctgattt gcattatacg
    10561 gtaaagccga aagatataca atgaggcaac agtgagtgcg gagatggcgg tgcgtgcacg
    10621 ggtgtcgtcg gggacaggca attcttcccc tgggaacgcc aatctccgca ttggcacaca
    10681 cgcggatcat gacgatgacg gtcactcgga ttcgatgcgg aaatacgtcc cgcgacatgc
    10741 ctcctccatc agtccgacac acgacgctgt cattgctccc ttccgacgtt
//
LOCUS       DAOPWB010000107        10677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6265_length_10677_cov_5.536528, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000107 DAOPWB010000000
VERSION     DAOPWB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6265_length_10677_cov_5.536528"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PKN92_09730"
     CDS             complement(<1..216)
                     /locus_tag="PKN92_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49114.1"
                     /translation="MTLCDCGAKRAVDAQADHDEESRFNPQRNHVPGHIFSVGKGRSG
                     AVDGVTPAPLVIPAKAGILTRVALDEGR"
     gene            261..1856
                     /locus_tag="PKN92_09735"
     CDS             261..1856
                     /locus_tag="PKN92_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HNZ49115.1"
                     /translation="MADKKKVYVVSNGDFRIPACVNGWPLQKKTLGQLQTALKKLGYD
                     AEILPKYNAKDKHGFLTKQCEGTAAFARIDPKAPVIVLLNIWAYAHHVSGPLQTHNGP
                     ILLLANFDGTYPGLVALLNHSASLDRLNVRHSRLWSESFSDDPFFMKHLEKWLKTGKI
                     VHNESHMIDDAQVSISKGAETLGKDLAASIKKERRIMGQFDPGCMGMLNAVMDPAKLG
                     AAGMPIEYLNQSDLLAEMNLVSDEEAQSYLDWLLKKGTTFHWGTNKFTDLVYDQVLTQ
                     MKMYAAACRFVARYGLSTIGIPYQYGLVRCVPASDLVEGMLNNADRPPVRDPETNRIV
                     FPGKPIPHFNEGDIGAGVPQLLMSEIFAAKKMPQETTLHDVRWGREYDGKFVWVFLIS
                     GGAPPAHFGGWKNTHVYRQPSGYFPLGGGTCSGVSKPGVITWARCYEAYGEMGMDCGT
                     GEVVDLPEKEVRERLDKTDPQWPIANVYMPGYGRDQLMASHMSNHIVIGYGDILQELI
                     ALCRNLGIKVRVAGDAGKALKKK"
     gene            2004..4655
                     /locus_tag="PKN92_09740"
     CDS             2004..4655
                     /locus_tag="PKN92_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ49116.1"
                     /translation="MNITELGGYGIPASLISQWRTLQGEKLLPLQEKALRNFGLFEEG
                     NLLVQAPTSSGKTFVGELAAVRAALAGKKSAFLLPLKALAEEKYREFCNKYEAYGIRT
                     IICTRDHRRFDAAFEEGRYDMAIAVYEKLEQLAVTRPERLQELSLVIADELELLSDPE
                     RGDQAEVLLTRLLQEGVRIIGLSAVLGDPGALAHWLQARLLEQDRRPLELHYGVLFDG
                     VYRYCGHNNPQESEERWESGQGDSSWSEVVHAVRRLADAGESCLVFVKARREACRGAE
                     LLARHLQLPAATEAREALSVLAPTRARNLLLQTLETGTAFHSADLLTEERRIVEEAFR
                     RGEIRVLMATGTLAAGMNLPARNVFVSVEKWIYDPRLDLPWRAPISQGEFENMSGRAG
                     RYNGAAEEGRAVLVATTRFERDALWQRYIKGQREPVQPRLDKASLDDAVLQLIASQKA
                     ATLEELTDFFLATLSARLVWDGFYNTEQIRFRVRSALQRCLEAGAVSARNEQGASVAP
                     QPDEPVGALFFEAAPSGRVIAAKGVSLPSFRAILHWLRLSEQRDWYPLDLLVMLALLP
                     DARLRQVPLSRREYEKDEYPRRMKEATKGQELRVDIPVNRLRNCRIMPFYDEARGIKT
                     ALFLCEWLEGAALETVEEHFDVSAGQITAAADQLAWLADATSVLAEAEGFNPAFVLSL
                     RHFTVRLQFGIRDALVPLAEAVPSLTRNALLALAAEGLDTWETLHCASAALLERWMSS
                     REAAALQRLATVKMGERETRNGKKETASSNDTAPLLVIDDRLPGQIQWQERIVPLQEK
                     QYRLIRTLAAHPGECVAYDRVYRQIWGDLVVEENQMHYQKRMLLQRLEKADPAWKTLI
                     RTIPKRGFMLSLPSRQVQLTRLPAGQG"
     gene            complement(4668..5450)
                     /locus_tag="PKN92_09745"
     CDS             complement(4668..5450)
                     /locus_tag="PKN92_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNZ49117.1"
                     /translation="MNTVLSCLCGRFDRRSIKIYLASMSVLFWSLIFLAWLGYPTEHK
                     YSIMTHTFSFLGSYDPQHSPVWWWLFTVALILWGMTTIPLVFYIHRHFSELSTAGAHT
                     GAACLLTGAVCIMLVGIFPDVKTPLTSTLRVTDVHEKVALLAAAGFILGNFTHGFLLL
                     KDRFSGRQNLFVHRYFAVLYGIWLSILFTASYFLIKWEFVYAQMKAAAQATGQPIGSS
                     WSEAMNTIYSFPLWENILIYSFFVFLVSKTLILSSDGPAVED"
     gene            complement(5521..6462)
                     /locus_tag="PKN92_09750"
     CDS             complement(5521..6462)
                     /locus_tag="PKN92_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49118.1"
                     /translation="MCLFEKRTGLVWCLFFLLPATVFCADDFGPGKRTVVEAHNCYPY
                     RGFWANRLDLALSCPFPVGVELDLLWHGAENEGQGRIVVAHGGPVDDRDYSLESWFFD
                     RVRPLVEESLAKDTADRWPLITLNLNDIRGHSEALYPELVSLIDRHGHWFCSAVKGAD
                     DAPASLRPAPVLLLTGGGAREKKYFYDAVPEGGVIRAFGSGSIHEKADNFRRWINYPW
                     SAVEPEGQPHAGAWTPEDAARLQKLVEEAHQQGYWIRFYTLNGHSAVSVVRNGWSPAY
                     NFGSPDAVRLRWTAARDAGVDFIATDQCVEAAAWLSR"
     gene            complement(6583..7194)
                     /locus_tag="PKN92_09755"
     CDS             complement(6583..7194)
                     /locus_tag="PKN92_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HNZ49119.1"
                     /translation="MAKEVIVVSGLPRSGTSLIMQMLQAGGVPLLTDHIRCADNSNPR
                     GYFEYEPVRFLKKDASWLAGARGSAVKIVSPLLKYIPYPCPCKIIVMNRPLTEVLLSQ
                     TRMLLQQAVHKGIETASALLEQTAAENEALRPLYAKHMKELQLQPSLRSDFSFLSLDY
                     HAVLKDPEEASEAITSFLGDDLDIAAMAAVVDPGLYRCRLDGD"
     gene            complement(7266..7844)
                     /locus_tag="PKN92_09760"
     CDS             complement(7266..7844)
                     /locus_tag="PKN92_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ49120.1"
                     /translation="MEPKQSFSTSDVAKYCHVTPDTIRKWSEAGRLHVFKTPGGHRRV
                     RREDLIQFMKTNKIPLHEDLKNYATKVLIVDDEKIVISVIRRFLAQLDTPFHVEIATD
                     GFDAGSLVGTFKPDVVFLDLRLPGIDGFEVCRRIKSSPHLQSPEVIAMTGYYDGETAK
                     RVLELGASLLVQKPFTPEDLATALEQVGVKIG"
     gene            complement(8023..9360)
                     /locus_tag="PKN92_09765"
     CDS             complement(8023..9360)
                     /locus_tag="PKN92_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HNZ49121.1"
                     /translation="MAEPVAASAKSPMFHVMAKPSGAACNLRCTYCFYLEKELLYPDR
                     GSIIMDDRVLDAYVRQTIEAQAHLPVVDFAWQGGEPTLAGLDFFRKAFALQQQYAEGK
                     EVYNSLQTNGILLDEEWADFLKEHEVLVGLSLDGPERFHNRCRQDVRGMPTFRQVMQA
                     LEVLHRKEVDFNLLACVNRYTSAAPEEVYPFLRAHGAGFIQFIPVVERRLAETVPGEL
                     TLPAPDDPREAAVTEWSVLPHDYGRFLVSVFNQWVCRDVGSTFVQLFDVTLEAWMGMD
                     PSLCFFKERCGDALILERNGDLYSCDHFVYPEHRLGNILETPMTQLAGSEQQRAFGDA
                     KRDRLPGQCRGCPVLHLCRGECPKHRFLTDSQGEAGLNYLCEAYINFFAHTGPFMRFM
                     AEEIRNQRPPAGVMAWIQAQQMESAGKRSPAPNDPCPCGSGRKFKKCCGTAKG"
     gene            complement(9353..10246)
                     /locus_tag="PKN92_09770"
     CDS             complement(9353..10246)
                     /locus_tag="PKN92_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNZ49122.1"
                     /translation="MADLLNLLAERKLLVSDGGWGSFLIAAGMQPNECPERWNLDRPD
                     QIRAIAAMYAEAGADLVMTNSFGGSSLKLAPYGLADQAKAINEAAARLTRESIGEGRV
                     VLASMGPTGKLLMMGDVGESEIYDSFAEQARALEAGGADAIAVETMSALDEAALGVRA
                     VKENTGLTVVASFTFNHHEETGYRTMMGVTPEEMAAAMLAAGADVLGVNCTLCAGEMV
                     ALAAAVRCAAPDTPLLVHPNAGQPQRKADGSVFYLETPEELAAQVPALAEAGASIIGG
                     CCGTGPDHIRAIRAAVERIHG"
     gene            10409..>10677
                     /locus_tag="PKN92_09775"
     CDS             10409..>10677
                     /locus_tag="PKN92_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010855040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ49123.1"
                     /translation="MLTLCAVLFPAPGEGTHTCAHRGNVTDFPENTLPAIQAAVTLTA
                     DQIEFDVHMTKDGQLVIIHDSTVDRTTDGSGSIADMTFEEVRALDA"
BASE COUNT         2561 a         3137 c         2812 g         2167 t
ORIGIN      
        1 acgaccctca tcaagagcaa ctcgcgttaa gattcctgcc ttcgcaggaa tgacgagggg
       61 ggcgggcgtc actccgtcca cggcgccact cctgcctttc cccacggaaa agatatgtcc
      121 cgggacatga ttccgctgcg gattgaagcg actctcttcg tcatgatccg cttgtgcgtc
      181 aacagctctt tttgcaccgc agtcacataa cgtcatacaa tggctgagca cacaacaaaa
      241 cccatcagga gatttctatt atggctgata agaaaaaagt gtatgtcgta tccaacggtg
      301 acttccgtat ccccgcctgt gtcaacggct ggcccctgca gaagaagacg cttggtcaac
      361 tgcagacggc actgaaaaag ctcggctacg atgccgaaat actgcccaaa tacaatgcca
      421 aagacaagca cggatttctc accaaacagt gcgaaggcac cgccgctttt gccaggattg
      481 atcccaaagc gcctgtcatt gtgctgctga atatctgggc gtacgcacat catgtgtccg
      541 gcccgctcca gacacacaac gggccgatcc tgcttcttgc caacttcgac ggcacctatc
      601 ccgggctggt ggcactcctc aaccactccg cctcccttga ccggctcaat gtcaggcatt
      661 cccgcctctg gagcgaaagc ttcagcgacg accccttctt tatgaaacat ctggagaaat
      721 ggctgaagac agggaaaatt gttcacaacg aaagccacat gatcgatgat gcacaagtga
      781 gcatctccaa aggagcggaa actctcggca aagacctcgc cgcatccatt aaaaaagaac
      841 ggcgtatcat gggtcagttt gatcccggat gcatgggcat gctcaatgcc gtcatggatc
      901 ccgccaaact cggtgccgcc ggaatgccca tcgaatatct caaccagagt gacctgctcg
      961 cagaaatgaa tctggtgagc gacgaagaag cacagagcta tctcgactgg ctgctgaaaa
     1021 aaggcaccac cttccactgg ggcaccaaca aattcaccga cctcgtttat gatcaggtgc
     1081 tcacgcaaat gaaaatgtat gccgccgcct gccgcttcgt ggcgcgctac ggactcagca
     1141 ccatcggcat tccctatcag tacgggctgg tacgctgcgt gcccgcttcc gacctggtcg
     1201 aaggcatgct caacaacgcc gaccggccgc ccgtgcgtga tccggaaacc aacagaatcg
     1261 tcttccccgg aaaacccatt ccccatttca acgaaggcga tatcggcgcc ggcgtgccgc
     1321 agctgctcat gagcgaaatc ttcgcagcga aaaaaatgcc gcaggaaaca acgctccatg
     1381 atgtccgctg gggcagggaa tatgacggca aatttgtctg ggtcttcctc atctccggcg
     1441 gtgcgccccc ggctcacttc ggcggctgga aaaatactca tgtgtaccgc cagccttccg
     1501 gctacttccc cctcggcggc ggcacctgct ccggcgtgtc caagcccggc gtgatcacct
     1561 gggcgcgctg ctatgaagcc tacggcgaaa tgggcatgga ctgcggcacc ggtgaagtcg
     1621 ttgacctgcc cgaaaaggaa gtgcgtgagc gcctcgacaa aacagatccg caatggccta
     1681 ttgccaatgt gtacatgccc ggctacggac gcgatcagct catggcgtcc cacatgtcca
     1741 atcacatcgt catcggctat ggcgatatcc tgcaggaact gattgccctg tgccgcaacc
     1801 tcggcattaa agtacgcgtc gcaggcgatg caggcaaagc gctgaaaaag aaataagcac
     1861 aatctctccc ggcccctccc ggcgcaccgg cactcagcgg tgcgcctttt ttcatgcccc
     1921 ggttttttcg tctcttcctt gacaactgaa taaatattca gtatactgat aagaccaact
     1981 tatggaattg aggagctgcg gacatgaata taacagaact gggcggatac ggaatacccg
     2041 cctcactgat cagccaatgg cgcacattgc agggcgaaaa gctgctgccg ctgcaggaaa
     2101 aggcgctgcg taatttcgga ctctttgaag agggcaatct gctggtgcag gcgcccacca
     2161 gttcaggaaa aacctttgtc ggtgaactgg cagctgtccg tgcggcactg gcagggaaaa
     2221 aaagcgcctt tctgctgccc ctgaaagcgc tggcagagga gaagtaccgg gagttctgca
     2281 acaagtatga agcctacggc atccgcacca ttatctgtac ccgggaccat cgccgctttg
     2341 acgctgcttt tgaagaaggg cgttacgaca tggccatagc ggtgtatgaa aaactggaac
     2401 agctggcagt cacccgtccc gaacggcttc aggaactttc gctcgtaatt gccgacgagc
     2461 tggagctgct ctccgacccg gagcggggcg atcaggcgga agtgctgctg acccggctcc
     2521 ttcaggaggg tgtgcgcatc atcggcctct ctgccgtact gggcgatccg ggcgctcttg
     2581 cacattggct gcaggcgcgc ctgctcgaac aggaccggcg cccgctggag ctgcattacg
     2641 gcgtgctctt tgacggtgta taccgctatt gcgggcacaa caatccgcag gaatcggaag
     2701 agcgctggga aagcggacag ggcgacagca gctggtcgga agtggtgcac gctgtacggc
     2761 gtctggcaga tgcgggagaa agctgtcttg tttttgtgaa agcacggcgc gaagcctgcc
     2821 ggggagcgga actgcttgcc cgtcatctgc aactgcccgc cgccactgaa gcacgggagg
     2881 ccctgtcggt actggcaccc acccgcgcac gcaatctgct gcttcagacg ctggaaacag
     2941 gcactgcttt ccacagtgcc gacctgctta cggaagaacg gcgcattgta gaggaggcat
     3001 tccgtcgggg agaaatccgg gtgcttatgg caacaggcac gcttgccgcc ggcatgaatc
     3061 ttccggcacg caatgtcttc gtcagcgtgg agaaatggat ttacgatccc cggctggacc
     3121 ttccctggcg cgccccgatc tcgcagggtg aatttgaaaa catgagcggc cgggcagggc
     3181 gctacaacgg ggcagcagaa gaagggcggg cggtgctcgt tgccacaacc cggtttgagc
     3241 gggatgcgct ctggcagcgt tatatcaagg ggcagcggga acccgtgcag ccacgccttg
     3301 acaaggcttc cctcgatgat gccgtactgc agctgatcgc ttcacaaaaa gcagcgactc
     3361 tggaagaact gaccgacttc tttttagcca cactttccgc ccggctcgtc tgggatggtt
     3421 tttacaatac ggaacagatc cggttccggg tgcgctccgc cttgcagcga tgcctggagg
     3481 caggtgccgt gtccgcccgg aatgaacaag gcgcttccgt agcaccgcag cccgatgaac
     3541 cggtgggcgc gctttttttt gaagcggcgc cttcaggccg ggtcattgcc gccaaagggg
     3601 tttcccttcc ttctttccgt gccatcctgc actggcttcg cctctccgaa cagcgcgact
     3661 ggtacccgct ggatctgctg gtcatgctgg cactgctgcc tgatgcacga ctgaggcagg
     3721 tgccgctgtc acgccgggaa tatgaaaaag acgagtatcc ccgccgtatg aaggaagcaa
     3781 ctaaagggca ggaactgcgg gtggatatac ctgtcaaccg tttacgtaac tgccggatca
     3841 tgccttttta tgacgaggca cggggcataa aaacagcgct ctttctgtgt gaatggcttg
     3901 agggagcagc tctcgaaacg gtcgaggagc actttgacgt gagcgccggc cagatcaccg
     3961 cagccgctga tcagcttgcc tggctcgccg atgccacttc cgttctggcg gaggcggaag
     4021 gattcaatcc tgcttttgtt ctttcgctcc ggcacttcac tgtgcggctc cagttcggca
     4081 tcagagacgc attggttcct ctggcggaag cagtgccttc actgacacga aatgctcttt
     4141 tagccctcgc tgcagaaggc ctcgacacct gggaaacgct gcattgtgca agcgcggcac
     4201 ttctggaacg gtggatgagc agcagggaag cggcagcact ccagagactt gcgacagtga
     4261 aaatgggcga aagagaaaca aggaacggga aaaaagaaac ggcatcctca aacgacacgg
     4321 ctccgcttct cgtaattgat gaccggcttc ccggccagat tcaatggcag gagcgaattg
     4381 ttccgcttca ggaaaaacag tatcgcctga tccggacgct ggcagctcat ccgggcgaat
     4441 gcgtcgccta tgaccgtgtt tacaggcaga tatggggcga tctggttgtg gaggaaaatc
     4501 agatgcacta ccagaagcgc atgctgctcc agcggctgga aaaagcggat cctgcgtgga
     4561 agacactcat acgcaccata ccgaagcgcg gatttatgct gagcctgccc tcccggcagg
     4621 tgcagctgac ccggcttccg gccggacagg gataagcccg aatgccgtca gtcttcgacg
     4681 gcaggtccgt cgctgctcag aataagggtc ttggacacca gaaacacaaa gaagctgtaa
     4741 atgagaatgt tctcccagag cggaaaagaa tagatggtat tcatcgcttc agaccaggaa
     4801 ctaccaatgg gctgacctgt tgcctgagcc gccgctttca tctgcgcata gacaaattcc
     4861 cacttaatca gaaagtagga agcggtgaac aatatggaaa gccagattcc gtaaagcaca
     4921 gcaaagtaac gatgaacaaa aagattttgt cgtcctgaaa agcgatcttt cagcagaaga
     4981 aaaccgtgcg taaaattgcc cagaataaaa ccggcggccg ccaggagcgc cactttttca
     5041 tgcacatccg ttacccgcag cgtggaagtc agcggcgttt tcacgtccgg aaaaataccc
     5101 accagcatga tgcagacggc gccggtaagc aggcaggctg cccccgtatg cgcccctgct
     5161 gtggacagct cgctgaagtg gcggtgaata taaaaaacca gcggaatagt cgtcatgccc
     5221 catagaataa gggcaacagt aaaaagccac caccagaccg ggctgtgctg cggatcataa
     5281 ctcccgagaa agctgaaggt atgggtcatg atcgagtatt tgtgctcggt cggataaccc
     5341 agccaggcaa gaaaaatcag actccagaac agcacactca ttgacgccag ataaattttt
     5401 atggagcggc ggtcaaagcg gccgcataga caggagagca cggtattcat cgctttcttt
     5461 ccttactgtg ccatgcagtt ttccggagat catacaggaa acacagcatt gctgcaaaaa
     5521 tcaccgtgag agccaggcag ccgcttcaac acattgatcc gtagcgatga aatcaacacc
     5581 cgcatcgcgg gcagctgtcc agcgcaaacg gactgcatcc ggagatccaa agttataggc
     5641 gggcgaccag ccgttacgca caaccgaaac cgctgaatga ccgttcagcg tataaaaacg
     5701 gatccagtaa ccctgctgat gcgcctcctc aaccagcttc tgcaggcgcg ccgcatcttc
     5761 cggagtccac gcacccgcat gaggctgacc ttccggctcc actgcgctcc agggataatt
     5821 gatccatcgg cggaaattgt ccgctttctc atgaatcgag ccggagccga aggcacggat
     5881 cacgcctccc tccggcaccg catcataaaa atacttcttc tcgcgggcac cgcctccggt
     5941 aagaagcagc acaggtgccg ggcgaaggga tgccggagca tcatctgctc ccttcacagc
     6001 actgcaaaac cagtgtccat gccggtctat cagtgagacc aactccggat ataacgcttc
     6061 actatgtccc cgtatatcgt taaggttcag cgtgatcagg ggccaacgat ccgcagtgtc
     6121 tttggcaagg ctttcctcta caagagggcg aacccgatca aaaaaccaac tttccagcga
     6181 atagtcccgg tcgtcaacag gaccgccatg ggcgacaaca atcctgcctt gtccctcatt
     6241 ctcggcgcca tgccacagaa gatccagctc aacgcccact ggaaagggac aactcaaagc
     6301 cagatccagg cggttcgccc agaaaccgcg gtaaggatag cagttgtggg cttccaccac
     6361 agttcgcttc cccggaccga aatcatcggc acaaaaaacg gtcgcaggca acaaaaaaaa
     6421 gagacaccac accagacctg tacgcttctc aaaaagacac ataacttctc tcccgtggct
     6481 gtgaccatca ttggacttat ttcagcaacc cgaagattcg ccgaagcacc cttcctgaag
     6541 ccgccctggt ctcagcagat acaaagaaaa tacatgattt tcttaatccc cgtccagtcg
     6601 gcagcgatac aaacccggat ccaccacagc agccatagca gcgatgtcca ggtcatcgcc
     6661 gagaaaggat gtaatcgctt cagatgcttc ttccggatct ttcaggacag cgtgataatc
     6721 gagcgataaa aaagaaaagt cgctgcgcag cgacggctgc aactgcagct ccttcatgtg
     6781 ctttgcgtac agcggccgga gcgcttcgtt ttccgccgcc gtctgctcca acaaagcgct
     6841 ggctgtttca atgcctttgt gaactgcctg ctgaaggagc atccgggtct gggaaaggag
     6901 tacttccgta aggggccggt tcatcacaat aattttacag ggacagggat aaggaatgta
     6961 tttcaggaga ggtgaaacaa tctttacagc actaccccgg gcgcccgcca gccaggaggc
     7021 atcttttttc agaaagcgga caggttcata ttcaaaatag ccgcgagggt tgctgttatc
     7081 ggcacatcgg atatgatctg tcaggagggg aacaccgccc gcctgaagca tctgcatgat
     7141 cagagaagtt ccgctgcgag gaagaccgga aacgacgatg acctctttcg ccatggtctg
     7201 cgctttcttc tctgcggctt ttccagcggc accttttccg gctgccgaca aattaaggga
     7261 cacggttaac cgatttttac gcccacctgc tccagagctg tcgccagatc ttcaggggta
     7321 aaaggcttct gaaccaggag agaggcgcca agctccagca cgcgttttgc cgtttcaccg
     7381 tcataatatc ccgtcatggc gatcacttca ggagactgca ggtgcgggct gcttttaata
     7441 cggcggcaga cttcgaagcc atcaatgccg ggcaggcgca ggtcgaggaa aacaacatca
     7501 ggtttgaagg tgcccaccag gctgcctgca tcgaaaccgt ccgtagcgat ctccacgtga
     7561 aagggcgtat cgagctgagc cagaaaccga cgaattacag agatcacgat tttttcgtcg
     7621 tccacgatca gtactttcgt ggcatagttc ttcaaatctt catgaagcgg gattttattg
     7681 gtcttcatga actggatcag gtcttcacgc cggacccgcc tgtgtccgcc gggtgtttta
     7741 aaaacatgaa gacgtcccgc ttcggaccat ttacggatgg tatcaggggt cacatgacaa
     7801 tattttgcaa catcagaggt actgaaggac tgtttaggtt ccatgatcgg cactttcctg
     7861 aattaattgt atttcaactt tatgtacgag aatatgatac aggtatggct tcgctttttc
     7921 caattcatcg gtttgccagg aaatgaggca aaaccgcacc gcctcccttc tcctcacagt
     7981 ataacacctg attgcccctg aagatttcag aaaagagccg gatcagcctt tggcagttcc
     8041 gcagcatttt ttgaatttcc tgccgctgcc gcaggggcac ggatcattgg gggcagggct
     8101 cctttttcct gcagactcca tctgctgtgc ctgaatccag gccataacgc cggcgggcgg
     8161 gcgctggttc cggatttctt ccgccataaa acgcataaac ggccccgtat gggcaaagaa
     8221 gtttatgtac gcctcacaga gataattcag ccccgcttcg ccctgagaat ctgttaaaaa
     8281 gcgatgcttt gggcattccc cccggcacag atggaggacg ggacagccgc ggcattgccc
     8341 gggcagccgg tctctcttcg catcgccgaa agcccgctgc tgctccgacc cggcaagctg
     8401 agtcatgggc gtttccagaa tattccccag ccggtgctca ggataaacaa agtgatcaca
     8461 ggaatacaga tcgccgttgc gctccagaat cagcgcatca ccgcagcgtt ctttgaaaaa
     8521 acagagactg ggatccatgc ccatccaggc ttccagagtc acatcaaaaa gctggacaaa
     8581 agtggagcct acatcacggc aaacccactg attaaaaaca gaaacaagga acctgccgta
     8641 atcatgaggc agcacactcc attccgtcac agccgcttcc cggggatcgt ccggcgcagg
     8701 cagggtcagt tcgcccggca ccgtttccgc aagacgacgc tccaccaccg ggataaactg
     8761 aatgaatccg gcaccatgag cacgaagaaa aggatacacc tcctccggtg cggcggaagt
     8821 atagcgattg acacaggcaa ggaggttaaa gtccacttct ttccggtgca gcacctccag
     8881 tgcctgcatc acctgacgga aagtgggcat accccgtaca tcctggcggc accggttgtg
     8941 aaagcgctca ggaccgtcga gcgacagacc taccagcacc tcatgttctt tcagaaaatc
     9001 tgcccattcc tcatccagca gtataccgtt ggtctgcagc gaattataga cctctttgcc
     9061 ttccgcatac tgctgctgaa gtgcgaaggc ctttctgaaa aagtccagcc cggcaagcgt
     9121 cggttcaccc ccctgccagg caaaatcaac aaccggcaga tgcgcctgcg cctcaatcgt
     9181 ctggcgtaca taggcatcca gtacccggtc atccataatg atggagcccc gatccggata
     9241 gagcagctct ttttccagat aaaaacagta cgtacagcgc agattacagg cggcgccgga
     9301 aggtttcgcc atcacatgaa acatgggaga tttcgcagaa gcagcaacag gttcagccat
     9361 gaatacgctc cacggcagcg cggatcgcac gtatatgatc cggtcccgtg ccgcagcatc
     9421 cgccgataat ggacgccccc gcttctgcca gagcaggcac ctgcgccgcc agctcttccg
     9481 gcgtctccag ataaaaaaca ctgccgtccg ccttgcgctg aggctgcccc gcattgggat
     9541 gcaccagcag cggtgtgtcc ggagcagcac agcgaaccgc agctgccaga gcaaccattt
     9601 ctcccgcaca aagggtacag ttcacgccca gcacatcagc gcctgccgcc agcatggccg
     9661 ccgccatttc ctccggcgta acccccatca tcgtgcggta ccctgtttcc tcatgatgat
     9721 taaaagtgaa ggaggcaaca accgtcagcc ctgtattttc tttcacagcc cgcacgccca
     9781 gtgctgcttc gtccagcgcg gacatggttt ccaccgcaat ggcatcggcg ccgcccgctt
     9841 ccagtgcccg tgcctgctcc gcaaaactgt cgtagatttc actttcacct acatcgccca
     9901 tcatcagcag cttgccggta ggtcccatgg atgccagaac cacccgcccc tcaccgatgg
     9961 attcacgggt cagccgcgcc gccgcctcat taattgcttt cgcctgatcc gccagaccgt
    10021 agggcgccag cttcaatgaa gagccgccga aactgttggt catgaccaga tctgcccctg
    10081 cttccgcata catggcggca atagcacgga tctgatcggg ccgatccaga ttccagcgtt
    10141 cggggcattc attgggctgc atccccgctg ctatcaggaa agaaccccag ccgccatcgg
    10201 acacaagcag tttccgttct gccagtaaat tcagtaaatc agccatgaca tatctcctga
    10261 gttttcccac cgccacctac tgtaaaacac cgggaatacg cttcgcaact ttacactgcc
    10321 cttgcgttgc agtcgttttt tctggtatac ccgaaacagc caatatacac cagggctttg
    10381 gagttcggcg attatgattc ttcacgttat gctcacgctc tgcgcagtcc tttttcccgc
    10441 accgggagag ggaacgcata cctgtgccca tcgcggcaat gtcaccgatt ttccggaaaa
    10501 taccctgcct gccattcagg cggctgtcac attgacggca gaccaaattg aatttgatgt
    10561 gcacatgacg aaagacggtc agctggtaat catccacgac agtaccgttg atcggaccac
    10621 cgatggaagc ggcagcattg cagatatgac ttttgaggaa gtgcgcgccc tcgatgc
//
LOCUS       DAOPWB010000108        10444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6428_length_10444_cov_6.149293, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000108 DAOPWB010000000
VERSION     DAOPWB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6428_length_10444_cov_6.149293"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="cmk"
                     /locus_tag="PKN92_09780"
     CDS             complement(<1..665)
                     /gene="cmk"
                     /locus_tag="PKN92_09780"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNZ49124.1"
                     /translation="MTQNRVQDGTQVVAIDGPAGAGKSSVARAAAQVLGFSFLDTGAM
                     YRAATLRAMRQGVPLDDSEALARCTAAMTYEQQESDGGTRILLDGEDVSESIRSPEVT
                     RNIQHLDQIAAVRQHLVALQRDFGLRQPTVAEGRDMGTVVFPRSRCKIYMDASLECRV
                     MRRALEMEAKGLAVNRDLLLQEMKARDDSNIARKESPLRPAEDAVLLDTTAMTKEQVI
                     DTIV"
     gene            complement(714..1166)
                     /locus_tag="PKN92_09785"
     CDS             complement(714..1166)
                     /locus_tag="PKN92_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNZ49125.1"
                     /translation="MITRLEFIEASQIRIIPAGATKHEALEILVEALCQNPAIQNKEV
                     FTRAVHEREAIQTTGLGNGVAVPHVRIDEVSAPTVALGIAPDGLDFQALDNRPVYIIV
                     LFATPQDSGKTYLSLLAKVMVTLREEETFAALAACRTVEEVTSLLGIS"
     gene            complement(1190..2365)
                     /locus_tag="PKN92_09790"
     CDS             complement(1190..2365)
                     /locus_tag="PKN92_09790"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNZ49126.1"
                     /translation="MQIADRLGKIPPYLFMTLRNKINEARAAGIDVISLAIGDPVEAT
                     PANVVEALAAAARDKSNHCYPTDEEWGMRAFRDAVARWYARRYNVTLDPAREICALIG
                     SKEGCHHFALGVINPGDTVLVTDPGYPGYKPSIWFVGAEPYPVPMCVENDFLPDLSAI
                     PSDVAKKATAFYLNYPNNPTGAVATPEFLNDLVAFAKEYSIAICYDNPYSEVVFGVNR
                     LSFLNAPGAKEVGVELNSLSKPYNMTGWRIGMACGNPDIVRAIATSKANTDSGVFNAV
                     QYAGIEALDTCDDFIGEMLALYNKRREKILATLRELGWRFDPPKGTFYLWVPVPKGFT
                     SAEFCDYVFEKASVVLAPGAAYGANGEGFVRFSLTVSDDRLDEALSRMRSHLGALSF"
     gene            complement(2489..3553)
                     /locus_tag="PKN92_09795"
     CDS             complement(2489..3553)
                     /locus_tag="PKN92_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ49127.1"
                     /translation="MSLLMAVLAGVVLSCADSTPLYTDKSSLLYYLNEEGTSVPVSTV
                     TDWTIRRRHIVKHMEAVMGSLPGDDRSRPPAFEVLEESLLSGSVRKLIRYESAPGSPV
                     HAFLYLPEEPSEKRAAMLCLHPTSPLGKRVVAGEGERPNRNYAQELAAQGYVVLAPDY
                     PGFGDDAGSRKTLYEAGYVSCTMKGIWNHIRAIDLLQSLHEVDPERIGCIGHSLGGHN
                     TLFAGVFDERIKVLVTSCGFTAFPRYMGGDLRGWTHDGYMPCIAAVYENSPERVPFDF
                     PEVLGALAPRPVFINAPVHDDNFDVTGVQECVASAKALYALFNCESDLVALYPEAEHD
                     FPDTERQAAYAFIHKYLGEP"
     gene            complement(3558..4340)
                     /locus_tag="PKN92_09800"
     CDS             complement(3558..4340)
                     /locus_tag="PKN92_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNZ49128.1"
                     /translation="MNDRDHFAPIARFYDQMMEHVNYVRWERIADHLSALVPRPFLHL
                     DVGCGTGVFMELMRKNGWDTLGTDLSYSMLEVAGRSRGLAPLLQSNMCALPFSGSIGM
                     LTCLFDSLNFLLKNEEIEAAFRSFYTALTPGGLAYFDVVTERMIEAHFNNTSWEENFH
                     GFKSKWHSAWNAESRICETSIQINAGENSVTRERIYPLSFLLEAIARSGLTLLAVRDA
                     YSWHEAGRKSVRLEFVAVKGSSPLLEKKFAAIDKQIRRLCGG"
     gene            complement(4337..4921)
                     /locus_tag="PKN92_09805"
     CDS             complement(4337..4921)
                     /locus_tag="PKN92_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNZ49129.1"
                     /translation="MPIIQEPTTKAYLKLLRERLSDARLSHTVFTAEYLSSFAPDLGL
                     NHGDAVVAALFHDFYRDCAPEAMLQDARQLRISMSDCQLEEPLLLHGPLAAEWCRREL
                     EISEAVYEAIYWHTTGRPGLGLLGQALYVADFAEPSRRFPEAEQARQLLRRENFRTAL
                     LFTTEIRHAVNQQKKVTDPNTEAFLLWLKTGGAA"
     gene            complement(4912..5496)
                     /gene="nadD"
                     /locus_tag="PKN92_09810"
     CDS             complement(4912..5496)
                     /gene="nadD"
                     /locus_tag="PKN92_09810"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNZ49130.1"
                     /translation="MRTGVFGGTFDPIHNGHLAIARAAKEEAALDRVLFVVAANPPHK
                     KDNRLTAAPLRVQMTEAAIAEERGFQISLIEIERRGYSFTVDTLKSLGEEDPGGELFF
                     ILGYDSALDLPHWRDARLILDLATLLVAPRPFCADPLPALLRDRAILLKTEPWDISSS
                     DIRSRIRKNEQTDHLLPPSVLDIIRKKRLYQPCP"
     gene            complement(5500..6753)
                     /locus_tag="PKN92_09815"
     CDS             complement(5500..6753)
                     /locus_tag="PKN92_09815"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNZ49131.1"
                     /translation="MNLQQELQQLGRQARQASNALRSLSRGIKDEALRRVARDLRAAK
                     EKIKDANAKDLEAGRAKGLSDAMLDRLELTDARIEAMAEGLEVVAALPDPVGDIVDQY
                     VHPNGLRIARIRQPLGVVGIIYESRPNVTADAAALCLKSGNATLLRGGSEAIHSNIAI
                     AEIFSAGVQAAGVPTHAIQIVTTTDRAAVGELLKLDKYVDVIVPRGGKSLIARIYEES
                     RIPVVAHLDGICHTYLHEDADPAMAQKIVLNAKLQRPGVCNAMETLLIHESAAPMMLP
                     ALAEALQAGGCELRGCERTRQLIDCAAASEEDWETEYLDKVLSIRVVASFEEAVAHIN
                     EHGSHHSDAIVTESYLFAERFLDEVDSATVYVNASTRFTDGFEFGLGAEIGISTDKLH
                     CRGPMALEGLTSLKYVIRGTGQVRE"
     gene            6951..7238
                     /locus_tag="PKN92_09820"
     CDS             6951..7238
                     /locus_tag="PKN92_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49132.1"
                     /translation="MKQDTRHIAIAMTRSIAQIIEGATDQQVDFYKLLWNVHWAIDYY
                     GVDKTRSTLIEIVLDADFKADELATRLRDTLFQEQMKEDTLGDWFTHAMKD"
     gene            complement(7277..>10444)
                     /locus_tag="PKN92_09825"
     CDS             complement(7277..>10444)
                     /locus_tag="PKN92_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ49133.1"
                     /translation="KCMHSFLSQKRTSLCLRHLADYAAVILITVSALWGGATLCFSRG
                     GGMSSGTGLILTMLRPSILYAAGAGFYDADCSDEEFPALEAFFKQKIHEIPPEAFPSR
                     FSQALEVDPLYGCHVYLNVFMGVLFRLFGISNDTVHLACLILHAVSMVLLYFLFRTFL
                     GTMPACLTALYLSWSPALLIMVPELRDYGKFPFFLAVLLLLAHILKRKHPPLRLFSLA
                     AALGAVIGIGWGFREDLLVFAPIAAIVLLLISPCTEKAFLCRIAATGILLLTVLLVSY
                     PVRQLGGTGPVRTLHTLLAGITETPEQVIWFGDADYDFGYLDYDEPIVAAVSAFGQRH
                     EQCQISYWFDPEYVRSSRALFEKIVQTVPADFPARGVSMTAASFFISECNQAALKSDA
                     ILRHLGEHPWIHYAFYVHNAVNRLFNRLGLIAGMLILLLAGRRSLPRALVLFAVFLYF
                     SAYPSLLAEFRHFFHLFFVPLLLAGVCFTMAQEDVRTLRHAQKAEERSSLYAAYFRRL
                     ALALSFFMVLFLSAALCLQGLRLYQSNRVKALAAAYESLQLTPLDWSTESDEKNTRLL
                     LKQSFTERAGFSELAPVQTVPAFLAVKFKEENRFLRFSLLNTNSAFFKECHIRLQGTG
                     IFYFPAFDFGGEEEARFTGIEIANEDLPLVEGIYEAENAGQLPLWISMGLSGQRETMR
                     TWKSGLPDHWLHRLYLACCAIIDRSDERMMQRWQLFIRRFPEQTFYADRALRWAAQKE
                     NEGLTEQLWLLIGRYTRDRRAASAKWLHNQADAAFNRKDLHAAMALYQKVLEMAPYDL
                     WNLVCIGEIHQQEGNTDAAKACFQEVLIKAPESPYTAGWLQDLFEESHEPDADAFWVR
                     LIEMHPEAAVPWLYHGLALKRAGEMDRAKEAFQRSHGNNPQWGLPLVHLGDLLLGNGD
                     IVKGTEYLNHALTLQDTLAPEVAQAYSRAAQLFQEQGKLKEAVALSEKALEMAPYDLW
                     NLVRIGEIHQQEGNTDAAKACFQEVLIKAPESPYTAGRLQALFTAERDAQAEAFWRYL
                     AELHPEAQLPRDYLAKRGALYGAAE"
BASE COUNT         2443 a         2841 c         2837 g         2323 t
ORIGIN      
        1 acaatggtgt cgatgacctg ttcttttgtc atagcggtgg tatcgagcag cacggcatcc
       61 tctgccggac gcagcggcga ttctttccgg gcaatattac tgtcatcccg tgctttcatt
      121 tcttggagca acaagtcccg gttgacggcg agccctttcg cctccatttc cagggcgcga
      181 cgcatgaccc ggcattccag cgatgcatcc atatagattt tgcaccggga acggggaaaa
      241 acgaccgtgc ccatatcacg cccctccgca acggtaggct ggcggagccc gaagtcccgc
      301 tggagcgcca caagatgctg tcgtacagcg gcaatctgat cgagatgctg aatgtttcgg
      361 gtcacctccg ggctgcgtat gctttcggat acatcctcac cgtcaagaag gatacgggtg
      421 ccgccatcgc tttcctgctg ctcgtatgtc atggcggcgg tacagcgggc gagcgcttcg
      481 gaatcgtcca gcggaacgcc ctgccgcatg gcacggagcg ttgccgcccg gtacatggcg
      541 ccggtgtcga gaaaagagaa acccagcacc tgggctgcag cccgtgctac gctgcttttt
      601 ccggcacctg cgggcccgtc tatggcgacg acctgtgttc cgtcctgtac cctgttctgc
      661 gtcatgtttg ggttctttgc gttgtatgtg cctgataaaa atcggatatc aggtcagctg
      721 attcccagca gggaagtgac ttcctcaacg gtgcgacaag ccgccagtgc tgcgaaggtt
      781 tcttcttccc ggagtgtaac catcactttt gccagcagac tgaggtaggt ttttccggag
      841 tcctgcgggg tggcaaacag gacgataata tagacgggcc ggttgtccaa ggcctgaaag
      901 tcaagcccgt ccggtgcaat gcccagcgca acagtgggcg cgcttacttc gtctatacgg
      961 acatgaggca ctgcgacccc attgcccaga ccggtcgtct ggatggcttc tcgttcatgc
     1021 accgcccggg taaacacttc cttattctgt atggcagggt tctgacaaag cgcttccacc
     1081 agaatttcca gtgcttcatg cttggtcgct cctgcgggaa taatgcgtat ttgtgaagct
     1141 tcgatgaatt ccaaccgtgt gatcatggca aatgagctca catcctgtgt taaaaagaaa
     1201 gcgctccgag atggctgcgc attcgggaca aagcctcgtc caggcggtca tcgctgaccg
     1261 tcagtgaaaa acgtacaaat ccttctccat tagctccata agcggcaccc ggcgcaagca
     1321 ccacggatgc tttttcaaag acataatcgc agaattccgc tgaggtgaag cctttgggca
     1381 caggaaccca gagataaaag gttcctttgg gcggatcaaa acgccagccg agttcgcgca
     1441 gggtggcaag aattttttcc cggcgcttat tataaagcgc gagcatctct ccaataaaat
     1501 cgtcgcatgt atccagcgct tcaatccccg catactgcac ggcattgaag acaccgctgt
     1561 ccgtgtttgc cttggatgtg gcgatggcac ggacaatatc aggattgccg catgccattc
     1621 cgatgcgcca gccggtcata ttgtagggct tggagagaga gttcagctcc acgcctacct
     1681 ctttggcacc gggcgcattc agaaaactga gccggtttac gccgaagacc acttcgctgt
     1741 agggattgtc gtagcagata gcgatggaat attctttggc aaaagccacc aggtcattca
     1801 gaaattccgg ggtcgccacg gcgcctgtcg gattgttcgg gtagttcaga taaaatgcag
     1861 ttgccttttt tgccacatcg gatggaatgg ctgaaaggtc aggcaaaaaa tcattttcca
     1921 cacacatggg caccgggtac ggctcggcgc cgacaaacca gatgctcggc ttgtagcccg
     1981 gatagcccgg atcggtcacc agcaccgtat ctccggggtt aatcacgccc agagcgaaat
     2041 ggtggcaccc ttctttggag ccgatcagtg cgcaaatctc ccgggcggga tccagcgtga
     2101 cgttataacg ccgggcatac caccgggcaa cggcatcccg aaaagcacgc atgccccatt
     2161 cttcatcggt ggggtagcag tggttgcttt tatcccgggc ggccgctgcc agtgcttcca
     2221 ccacattggc cggtgtggct tccacagggt cgccgatggc aaggctgatc acatcaatgc
     2281 ctgccgcacg ggcttcattg attttattgc gcagggtcat aaacaaataa ggcggtattt
     2341 taccgaggcg atcggcaatc tgcatgtatg tttccttttt aatgagtaaa ttcttcgctg
     2401 atatccgggg aaacggatac ggttcgatta taatggtacc actttggcaa gtcaatagac
     2461 agtcttttat ccggcatgaa tctggaattc agggttcacc cagatattta tgaataaatg
     2521 cataggcggc ctgtcgttcc gtatcgggaa agtcgtgctc cgcttcagga tacagggcaa
     2581 cgagatcgct ctcgcaattg aacagcgcat aaagcgcttt tgccgaagcc acacattcct
     2641 gaacgccggt cacatcgaag ttgtcgtcgt ggaccggtgc gttgataaac acagggcgcg
     2701 gtgcgagtgc gccgagcact tcgggaaaat cgaaggggac ccgttccggg ctgttttcat
     2761 acacggccgc aatacagggc atatagccgt catgcgtcca gccccggaga tcgccgccca
     2821 tgtatcgggg aaaggcggta aagccgcagc tggtgacgag caccttgatc cgttcatcga
     2881 aaacaccggc aaaaagggtg ttgtgtccgc cgagagagtg gccgatgcag ccgattcgtt
     2941 ccgggtccac ttcgtgaagg ctttgcagaa ggtcaatggc gcggatatga ttccagatcc
     3001 ctttcatggt gcagctgaca tagcccgctt catacagtgt tttccgggaa cccgcatcat
     3061 cgccgaagcc gggataatca ggtgcgagca ccacatatcc ttgtgccgcc agttcctgtg
     3121 cataattccg gttggggcgc tctccctctc ccgccaccac tcttttgccc agcggcgagg
     3181 tcggatggag gcagagcatg gcggcgcgtt tttccgacgg ctcttcaggc agatatagaa
     3241 aagcatgcac cgggctgccc ggggcgcttt cgtagcggat cagcttgcgc accgaaccgg
     3301 aaagcaggct ttcctccagc acttcgaagg caggcggcct gctccggtcg tctccgggaa
     3361 gactgcccat aactgcttcc atgtgcttca caatatggcg gcggcggatc gtccagtctg
     3421 tcacagtgga gacaggaaca gatgtacctt cctcgttcag atagtaaaga agggagcttt
     3481 tatcggtgta aagcggtgtg ctgtctgcac aagacaggac aactccggcg agcacagcca
     3541 tgagaagaga catgagttta ccctccgcac aatctgcgga tctgtttgtc tattgccgca
     3601 aatttctttt ccagaagcgg actgcttccc ttaaccgcca caaattccag acgcacgctt
     3661 ttcctgcctg cttcgtgcca gctgtaggca tcgcgcacgg cgagaagggt gagcccggat
     3721 cgggctattg cctccaggag aaaggagaga gggtagattc tttcacgggt cactgaattt
     3781 tctccggcat tgatctgaat gctggtctcg cagatcctgc tttcagcatt ccaggcgctg
     3841 tgccatttac ttttgaatcc gtgaaagttt tcttcccagc tcgtgttatt gaagtgtgcc
     3901 tcgatcatgc gctccgtgac aacatcgaaa taggcaagcc cgcccggcgt cagtgccgtg
     3961 taaaaagatc tgaacgctgc ttcaatttcc tcatttttca gcaggaaatt cagcgaatca
     4021 aaaaggcagg tcagcatccc gatgcttccg gaaaaaggca gggcacacat attggactga
     4081 agcagcggcg caagaccccg gctcctcccg gcaacttcca gcatggaata ggacagatcg
     4141 gtgccgagcg tgtcccagcc gttcttccgc atcagttcca tgaacacgcc tgtgccgcat
     4201 cccacatcga gatgcaggaa cgggcgcggc accagcgcag acagatggtc ggctatgcgt
     4261 tcccaccgca catagttcac atgctccatc atctgatcat aaaatcgggc aatgggggca
     4321 aaatgatccc ggtcgttcac gcagcacctc ctgttttcaa ccacagaaga aaagcttccg
     4381 tattagggtc ggttaccttt ttctgctgat ttaccgcatg gcgaatttcg gtcgtaaata
     4441 gcagggctgt tctaaaattt tccctgcgaa gcaactgcct tgcctgctcc gcttcaggaa
     4501 agcgccgtga cggctcggca aaatccgcca cgtaaagcgc ctgcccgagc agaccgagcc
     4561 cgggccgtcc ggtggtatgc caataaatgg cttcatagac cgcctcgctg atctccagtt
     4621 ctctgcggca ccattctgca gccagaggcc catgcagcag cagcggttcc tccagctggc
     4681 agtcactcat ggagatgcga agctgccggg cgtcctgcag catggcttcc ggagcacagt
     4741 cccggtaaaa atcgtgaaac agcgcggcga caaccgcatc cccgtgattc agtcccagat
     4801 caggtgcgaa agaagagagg tattccgctg tgaaaacagt atgggacaga cgcgcatcgg
     4861 aaaggcgttc tctcaatagt ttgagatagg cttttgttgt cggctcctgg attatgggca
     4921 tggctgatac agtctttttt tgcggattat atccagtacg gaaggaggaa gcagatggtc
     4981 ggtttgttcg ttcttccgga tccggctgcg gatgtcggag gaggagatgt cccagggctc
     5041 agttttcagc aatatagccc gatcccggag cagcgcaggc aggggatcgg cacagaaagg
     5101 gcgtggtgcc acaagcaggg tcgccaggtc aagaatcagg cgggcatccc gccaatgagg
     5161 taaatccagc gcggaatcat agccgagaat aaaaaacagt tcgccgcccg gatcctcctc
     5221 gcccagtgat ttcagcgtgt ctaccgtaaa agaataaccg cgccgttcaa tttctatcag
     5281 gctgatctgg aagcctcttt cttcggcaat ggcggcttcg gtcatctgca cccggagcgg
     5341 cgccgctgtg agccgattgt cttttttgtg cggcgggttg gcagccacca caaacagcac
     5401 ccgatcaagg gctgcttcct cctttgccgc tcgtgcgata gcaagatgcc cgttgtggat
     5461 ggggtcgaat gtgcctccga aaacgccggt acgcatgggt tattcgcgca cctgcccggt
     5521 tccccggatc acatatttca gagaagtgag cccttccagt gccataggac cccggcaatg
     5581 cagcttatcg gtgctgatgc caatttctgc gcccagcccg aattcaaagc cgtctgtgaa
     5641 tcgggtggac gcattgacgt acaccgtggc gctgtccact tcgtccagga aacgctcggc
     5701 aaagagataa ctttccgtta cgatggcatc ggaatgatgg ctgccgtgct cattgatgtg
     5761 cgccacagcc tcttcaaagg aagcgacaac ccggatagac agaaccttgt ccagatattc
     5821 tgtttcccag tcctcttcgg atgcggcggc gcagtcaatg agctgccggg ttcgctcgca
     5881 gccacgcagt tcacagccgc ccgcctggag ggcttcagcc agagcgggga gcatcatcgg
     5941 ggcggcgctc tcatgaatca gaagggtttc catggcatta cagacgccgg gccgctgcag
     6001 tttggcattg agcactattt tctgagccat ggcaggatcg gcatcttcat ggagataggt
     6061 atggcagatg ccatccagat gagcgactac aggaatgcgg gactcttcgt aaatgcgggc
     6121 gatcaggctt ttgcctcccc ggggcacgat tacatccaca tacttgtcca gtttcagcag
     6181 ttcgcccact gccgcccggt cggtagtggt cacaatctgg atggcatgcg tcggaacgcc
     6241 tgccgcttgc actcctgcac tgaagatttc cgcaatcgcg atattggaat gaatggcttc
     6301 cgaaccgccg cgcaggagag tcgcattccc ggatttcagg cagagcgccg ccgcatcggc
     6361 agtcacattg ggcctgctct cgtaaatgat cccgacaaca ccgaggggtt ggcggatccg
     6421 ggctatacgc agtccgttcg gatgaacata ctgatccaca atatccccga caggatcagg
     6481 cagcgcggcg acgacttcca ggccctccgc catggcttct atccgggcat cggtcagttc
     6541 aagccgatcc agcatggcat cggaaaggcc tttggcgcgt cccgcttcca gatcttttgc
     6601 gttggcatcc ttaattttct ctttggcggc acgaagatcc cgtgcaaccc ggcgcagggc
     6661 ttcatctttg attccgcgtg ataacgaacg aagcgcattg gatgcctggc gtgcctgtcg
     6721 ccccaattgc tgcagttcct gttgtaaatt catggacgct cttccttgtg ggtctgttcc
     6781 ggcagcgcgc cggaaacttt tttaacatac tatcatgagc ccttttgaaa gtaaaaacac
     6841 tttctgaaaa ggggtgctcc gtctttcaat gaataaaaga ggaggaaaca gcgtttattc
     6901 aaataaacaa actgtctctg cgaagcattt caaaagggat atgatacagg atgaaacagg
     6961 atacaagaca tatagccatt gccatgacca ggagcattgc gcaaattatt gaaggtgcga
     7021 cagatcagca ggtggatttt tacaagcttt tgtggaatgt gcactgggcg atcgattatt
     7081 acggcgtgga taaaacgcgc agcaccctca ttgaaattgt tttggatgcg gattttaaag
     7141 cagacgagct tgccacccgc cttcgggata cgctttttca ggaacagatg aaagaggaca
     7201 ctctgggcga ctggtttacc catgccatga aagactgagc gccgccctgt ttttatcagg
     7261 cggtacctct ttgcttctac tctgcggcac cataaagtgc gccccgtttc gcaagatagt
     7321 cacggggcag ctgtgcttcc ggatgcagct ccgccagata ccgccagaat gcttccgcct
     7381 gcgcatctct ttccgccgtg aacagtgcct gcagccgacc cgctgtatag ggtgattccg
     7441 gcgctttgat cagcacttcc tgaaagcatg cctttgccgc atcggtattg ccttcctgct
     7501 ggtggatctc cccgatacgc accagattcc aaaggtcgta aggtgccatc tccagtgctt
     7561 tttcagacaa agcaacagcc tctttcagct tgccctgttc ctgaaacagt tgggctgccc
     7621 ggctgtatgc ctgcgcaact tccggcgcca gggtatcctg aagagtcagc gcatgattca
     7681 ggtattctgt accttttacg atatcgccat ttcccagcaa cagatcgccc aggtgcacaa
     7741 ggggcagacc ccattgcgga ttattgccgt gggaccgctg aaaagcctct tttgccctgt
     7801 ccatttcacc ggctcttttc agtgccagac cgtgataaag ccacggcaca gccgcttcgg
     7861 ggtgcatttc aatcaagcga acccagaaag catccgcatc cggctcgtga ctctcttcaa
     7921 acaagtcctg cagccaaccc gctgtatagg gtgattccgg cgctttgatc agcacttcct
     7981 gaaagcatgc ctttgccgca tcggtattgc cttcctgctg gtggatctcc ccgatacaca
     8041 ccagattcca aaggtcgtaa ggtgccatct ccagtacttt ttgataaaga gccattgccg
     8101 cgtgcaggtc ttttctgtta aaagccgcgt ccgcctgatt atgaagccat ttggcagagg
     8161 cggcacgccg gtctctggta tagcgaccga tcaagagcca cagctgttct gtaagacctt
     8221 cgttttcctt ctgtgccgcc cagcgtaagg ctctgtctgc ataaaaagtc tgttccggga
     8281 agcgccggat gaaaagctgc caccgctgca tcattcgttc atcgctgcga tcaatgatgg
     8341 cacagcaggc aagatacagg cgatgcagcc agtgatccgg cagaccgctt ttccaggttc
     8401 gcatggtttc ccgctgcccg gacaaaccca tggagatcca taggggcagc tgcccggcat
     8461 tttctgcttc ataaatgcct tccacaaggg gcagatcttc attggcaatt tctattccgg
     8521 taaaacgtgc ctcttcttct ccgccaaaat caaaagccgg aaaataaaag atacctgtac
     8581 cttgcagccg gatatggcac tctttaaaga atgcgctgtt cgtgtttagc agagaaaaac
     8641 ggaggaaacg gttttcttct ttgaatttga cggcaagaaa agcaggcaca gtctgcactg
     8701 gagcaagttc ggaaaatccg gcacgctctg tgaaactttg ctttaacagc agccgtgtgt
     8761 ttttttcatc ggattctgtt gaccagtcaa gaggtgtcag ttgtaaagat tcataggcgg
     8821 cggcaagtgc ttttacacgg ttggactgat acaagcgcag tccctgcagg cagagcgccg
     8881 cactcagaaa gagcaccatg aaaaaggaca gagcaagggc gagacgacgg aagtaggctg
     8941 catacaggga agatcgttcc tctgctttct gtgcatgccg cagcgtgcga acatcttcct
     9001 gagccattgt aaaacatacg cccgcaagga ggagaggcac aaagaacaga tggaaaaaat
     9061 gtctgaattc agccagaagg ctcgggtagg cactgaaata aagaaagacg gcaaaaagaa
     9121 caagagcacg cggaagggag cgtctgcccg ccagcagcag tatcagcatt ccggcgatga
     9181 gtcccaagcg gttgaaaagg cggttcacag cattgtgcac atagaacgca taatgtatcc
     9241 agggatgttc tcccagatgc cggaggatcg catcactttt caatgccgcc tgattgcatt
     9301 cggatataaa aaaagaggca gctgtcatgg aaacgccccg ggcgggaaag tcggcgggta
     9361 cagtctgcac aattttttca aacaatgctc tgctgctgcg tacatattcc ggatcaaacc
     9421 agtaagagat ctgacactgt tcatgtcgct gtccgaaagc actcacagcc gcaactatgg
     9481 gctcgtcata atcaagatat ccgaaatcat aatcggcatc gccaaaccag atgacctgtt
     9541 cgggtgtctc cgtaatacct gcaagcagcg tgtggagcgt gcgtaccgga ccggttccgc
     9601 ccagttgccg cacgggataa gataccagca gaacagtgag cagcaggatg cctgttgctg
     9661 caatacggca cagaaaagcc ttttcagtac aaggactgat aaggagaagc acgatcgccg
     9721 ctatgggggc aaagacaagc aggtcttccc ggaaccccca gccgataccg atgacagcgc
     9781 caagggcggc ggcgagagaa aaaagtcgga gaggcgggtg ctttcgtttc agaatgtgtg
     9841 caagcaggag cagtacagcc aggaaaaaag ggaattttcc gtagtcacgc agttcaggaa
     9901 ccataatcag gagcgcaggt gaccacgaaa gatataaggc ggtaaggcac gcaggcatag
     9961 tgcccagaaa ggtacggaaa agaaaataaa gcagcaccat ggagactgca tgcaggatca
    10021 ggcacgcaag atgcactgtg tcgttactga tgccgaagag ccgaaaaagt acgcccatga
    10081 aaacattcag gtatacatgg cagccgtaaa gcggatcgac ctcaagcgct tgggaaaacc
    10141 gcgacggaaa agcctcgggc ggaatttcat ggatcttctg tttaaaaaaa gcctccagcg
    10201 cggggaattc ctcatcggaa cagtcggcat cataaaaccc ggcaccggca gcgtaaagta
    10261 ttgacggacg aagcatggtt aaaataagtc cggtaccgct ggacataccg ccgccccgtg
    10321 agaaacaaag tgttgcgcct ccccataagg cgcttacagt gatcagtatg actgcggcat
    10381 aatccgccag gtgccgcagg cagagactgg tgcgtttctg tgaaagaaaa gaatgcatac
    10441 attt
//
LOCUS       DAOPWB010000109        10336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6525_length_10336_cov_5.239665, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000109 DAOPWB010000000
VERSION     DAOPWB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10336
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6525_length_10336_cov_5.239665"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PKN92_09830"
     CDS             <1..563
                     /locus_tag="PKN92_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNZ49134.1"
                     /translation="FHVMGTSVGRDLFATFGKELSSGASILANRIGILITLIASVLLA
                     YILPVYFESGIAIVARGTAIFMGLCAATFLPFFVGGLYTKRITRTAAIVGGLAGFIVS
                     LLWILFVHEKESSVLLLCNAVFGRPSFFGNARTGFIIWSEVDALFVGLPVSAVITFVL
                     SFCTKALPVEHIRRCFGQEQKEAPLS"
     gene            complement(582..1829)
                     /locus_tag="PKN92_09835"
     CDS             complement(582..1829)
                     /locus_tag="PKN92_09835"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49135.1"
                     /translation="MIDRITVLGGSSVYTPEFLVALLSHNIVVKELVLLGLPGQKLDI
                     VCRFCQRLVKRSGHITEVIAETDPEKAMAGASYIINQIRVGGMKARIRDERGPTQFDM
                     VGSERLGAGAITNALRTLPVVFNLAHCVDAVNPKAKIINLSNPVGILVEALNRYTSLN
                     VVGVSDLPGMYVEKIASLLGRNPDAIEVNYLGIYDLGWIQDVKVGGLSRMSQLLELVE
                     KSEDDLFDHSLIRMFRMIPVRVLGVYYRRHEVLRRQQTGSRFRGEVLYDAEQRILKMY
                     ARPGLNKMPDLTRQRNALWYDNTIIPLLQKYESGGPAEAILCVPNKGSITDLPEYSAV
                     EVPVCVSAKSTKPRKMGMLPRFISGLYNSIKESDRLVIEAVRHKSRDLALQSLAVNPF
                     VPSIVRATEYLDKVIRQEKLDLF"
     gene            complement(2149..3180)
                     /locus_tag="PKN92_09840"
     CDS             complement(2149..3180)
                     /locus_tag="PKN92_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNZ49136.1"
                     /translation="MLYLGMMLAVLTASDAISSDLLSAAPEKPVKVIFDTDLGNDIDD
                     ALALGVIHALQSRGECELLAVTITKDEEETAACADAINHFYGRGDIPVGIVKGGPTPE
                     KSKFTGVARLKDGEQWRFPHDLMSGNDAPDATAVLRKTLAAQEDHSVVIVQVGFSTNL
                     ARLLDSEADDISPLSGKELAARKVKYISIMAGAFTDIPGLPEHCEYNVVMDIPSAKRL
                     LEEWPTPVVLSGFEIGIAITFPAVCIDRDFAYAPRHPLPEAYQMYEPTPHERPCWDLT
                     SVLWAVRPDRGYFTLSPAGTVTVTDQGRTFFEAKDGGKHFYLLADAVQCARVREALAL
                     LATQPPDAR"
     gene            complement(3205..5187)
                     /locus_tag="PKN92_09845"
     CDS             complement(3205..5187)
                     /locus_tag="PKN92_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNZ49137.1"
                     /translation="MLRVFSVFLSVCLLLCSARSFAERSALYPADWSPGFHDGAGRFL
                     HDFSYAGYQNGKNLPDPATISPRFDVVAEFGADNQGRSDATAAIQAAIDACRSAGGGL
                     VYLPAGIYRCEGSLAVLASNIVLCGESASNTYVYFTQCHSTSSHAHLIFGGSVKQRDS
                     TPLAEDGEALSFDIRVTDSSAFSVGDDVTVGWTITDEFVAEHGMTGTWQAFNGEWQPF
                     FQRTITAIDSDSNPPVITLDVPLRYPAKLRDGAVLTRNPGYLSGCGVMNLAVSDAIER
                     DLAWSQRQTQIIQMTDVKDSYIYRIRSFQSPACVDPLEKHVQSGGIRINNSKRVTVAY
                     CQMENSQNRGDGGNGYLYEVRQSNEILYQDCVARKGRHNFVQNWGFGATGIVWLRCES
                     MDSAQTYLVLGEEVRLRAFSEYHHSLSMACLVDSCFFNDGWSAGNRRHYSTGGGHSAT
                     QCALWNTRGLDKAEIRSYNFGHGYVIGTKDIAVKTLGHEVTGGLTTGTEPWDYKELID
                     AADLLEPQSLYESQRNRRLGFDEQVLSVSPARDGIVQVPEGDAAELRLLVSHGRGTLR
                     YQWYRVSGDGIHEALTGETGDSLSFSLASEQDTGGYFCEVRDDFYTRTSPVIHLVVTE
                     NKSVSAGSPGVWISGVLAVVCFFIVMMREKSASLPA"
     gene            5453..8257
                     /locus_tag="PKN92_09850"
     CDS             5453..8257
                     /locus_tag="PKN92_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49138.1"
                     /translation="MSFHLKLGIFLIVSLVLLVSSTGFGDDHWGPCPASPAVVNPPML
                     SPFQSVLALRGEWDFVADTNLLGRHRMGKGPEWNEPDWSQVRTLHVPGCWEAQGVGEP
                     GISETWDPLFDRIPRPINHKYMGAGRYRKQIDIPKDWDGKRVWLKIGGVRTEAWIWVN
                     KKRVAHLNTFCGTYKYDITDHVVPGEQVEIIATVRNDSPSRKGCMAAYHRFGGFYRDI
                     ELEATPQTRIDDVWVQGRLDRKGARVHVSIRHEGNSDPGTLEVAAALATLNGERVATF
                     QHPITLNADGIADLAVETTLMPFHAWTPENPFLYLAEVVLLQNGVPVHGWVERFGVRK
                     LEVKGDRFYLNNEPFFMRGYGDDYIYPLTLISPPDREAHRKHLDIARRAGFNYVRHHT
                     HCEIPEFFEAADEAGILIQPELPYYHDITTEAFSFDPLRDLQELYRHYRRYVSFASYC
                     TGNEGHLGSPVDKEVYAWAKQNDPDRIMQHQDGGCNTPDNADYDTPNSYGGMATSIVP
                     WMPGTFDALTMPFIAHEYLNLGIKMDPRTEPLFTGVIPAPRSMENYEASLVQAGLNRT
                     WGDRCLRAAHGLQGYYQKQGIEQARLDPACDGYSYWTIIDVMVNQENTYTGQGFLNAF
                     WEPKEGGLTPEEFFAFNGPTVLLADLDGINAVLTAGESVAVPLWISHFAAEPLNDAVL
                     TWTLAAGGNPLLSGEFDAGSFSPGDVRQAGTITLETPSVVTPVKATLQVTVNKTDISN
                     HWDFWLFPARQEQPGNDIAATEDLFPEIARRYPGAAQLGTAEAEKASLVIASENHPAL
                     TDTSLTGKRVLIIGAAEGEPNIKLGWWSLGNQLGTAFESHPLFGDFPVEESISPLWFR
                     LIKKGMPLPLNAEEGQVEHIVIGEGRKNYFTYLYRQKQEHSLPRLHSHGLDLLNGTPE
                     ADSLLDNLLDYMRTDAFIQF"
     gene            complement(8487..8921)
                     /locus_tag="PKN92_09855"
     CDS             complement(8487..8921)
                     /locus_tag="PKN92_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49139.1"
                     /translation="MITFKKSIYAICLTGTLLFLVGCQTNSKSQILSASESQVALRQM
                     QSRAFDTTDIPKTLRTVIATLQDLSFVIDKADAELGSVSGTKLDGYALRMTVSVRPHG
                     ESQLLVRANAQYNITAVEDPAPYQQFFMALEKAMFLEAHQVE"
     gene            complement(8933..9469)
                     /locus_tag="PKN92_09860"
     CDS             complement(8933..9469)
                     /locus_tag="PKN92_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49140.1"
                     /translation="MIKKVLFLQMVCIVITLAAGCQSTKIPKDALLLKEESMQLRQAQ
                     TRSFDTVEEKVLLAAAAGVLQDLGFTLDESEMDLGVLVGSKDRDAREFGQAVGSFMVA
                     AIFGVKTSVDKDQKIRASLVTSLLDGERTKLRVTFQRVVWNNEGKISRTEAIEDIAIY
                     QEFFEKLSKSVFLEAHEI"
     gene            complement(9481..9933)
                     /locus_tag="PKN92_09865"
     CDS             complement(9481..9933)
                     /locus_tag="PKN92_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006960853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49141.1"
                     /translation="MKIRSIATLLSYAILLTALGCASAASHRAAVSDDNTGRLTVGTV
                     QKEIRVGMSGADVASVLGSPNVVTTDEERREVWIYDKISTERAYSSSSGGVAALVFAG
                     GASVAGGGGASYSASSGASSTTQKTLTVIIKFDSEGLVRDFAYHSSKF"
BASE COUNT         2490 a         2967 c         2478 g         2401 t
ORIGIN      
        1 agtttcacgt catgggcact tcggtcggcc gtgatctttt cgccactttc ggcaaagagc
       61 tcagcagcgg tgccagcatc ctcgccaacc gtatcggcat cctgatcact ctcatcgcca
      121 gcgtgctgct ggcttatatt cttcctgtct attttgaatc cggtattgcc attgtggcgc
      181 ggggcaccgc catcttcatg gggctgtgtg cggcgacctt ccttcctttt ttcgtcggag
      241 gactctacac aaaaagaatc acccgtaccg ccgccatcgt gggcggcctg gcaggcttta
      301 tcgtcagcct cctctggatc cttttcgttc acgaaaaaga atccagcgtg ctgctcctgt
      361 gcaatgctgt tttcggaagg cccagctttt tcggcaatgc ccgtaccggc ttcattatct
      421 ggtctgaagt ggacgccctg ttcgtcgggc tgcctgtctc cgccgtcatt acgtttgttc
      481 tgagtttctg cacaaaagcg ctgcccgtcg agcatatcag gcgttgtttc gggcaggagc
      541 agaaagaagc acctctttcc tgacagagaa ggatgtgccg atcagaaaag atccagtttt
      601 tcctggcgga tcactttgtc gagatactcc gttgcccgga caatggacgg aacaaaagga
      661 ttgaccgcca gcgactgaag tgccagatca cgggacttgt ggcgcaccgc ttcaataacc
      721 aaccggtcgc tttctttgat cgaattgtac aaaccgctta taaagcgcgg tagcataccc
      781 attttccggg gctttgtgct tttggcgctc acgcaaacag gcacttcaac ggcgctgtat
      841 tccggcagat ccgtaatgga acctttattg ggcacgcaca gaatcgcttc tgcaggtccg
      901 ccgctttcgt atttctgcag gagcgggatg atggtgttgt cataccacaa cgcattgcgc
      961 tgccgggtca gatccggcat tttattcaga ccgggccgcg catacatctt caaaatacgc
     1021 tgctccgcat cataaagtac ttcgccccgg aaacgtgatc cggtctgctg ccttctgagc
     1081 acttcatgcc tgcgatagta aacgcccagc acacggacag gaatcatccg gaacattcga
     1141 atcaggctgt ggtcgaaaag atcatcttca cttttttcaa ccagttccag aagctgactc
     1201 atacggctga gcccgccgac cttcacatcc tggatccagc ccaggtcata gataccgagg
     1261 taattcacct ctatggcatc gggattgcga ccgagaagag acgctatctt ctccacatac
     1321 atccccggca gatcgctgac ccctaccaca ttcaacgatg tataccggtt taacgcctcc
     1381 accagaatac ccaccggatt ggaaagattg ataatcttcg cttttggatt cacggcatcc
     1441 acgcagtgtg ccagattaaa gacgacggga agagtccgaa gggcattggt aatggcaccc
     1501 gcccccaacc gctcgctgcc gaccatatca aactgggtcg ggcctctctc atcccgaata
     1561 cgcgctttca tcccgcccac ccggatctgg ttgatgatgt aggatgcgcc cgccatggcc
     1621 ttttccgggt ctgtctccgc tatcacctcg gtaatgtgac cgctgcgctt gaccagacgc
     1681 tggcagaacc ggcagacaat gtccagtttc tggccgggca ggccgagcaa caccagttcc
     1741 ttgaccacaa tattgtggga aagcagcgcc acaaggaact ccggcgtata tacgctgctg
     1801 ccgccaagta ctgttattcg gtcgatcatg gctacgcctc acggtcgtga aaagggcagc
     1861 cgaactgccc ttccggatgg ttgccagggg catgaaatat gcttcagaca aggcgcacac
     1921 agcgatccga atcgccatac gggtatcaga cacggacatc tctgctgaaa cgaaacaccg
     1981 ttgcctgcac cggctccaaa tactgagcct catgcccgac tgcactcaat ctaaagtatt
     2041 tagaggggta gtgtcaatat tttactcaat aaaaaagcct gcccggatct gcttctttct
     2101 ccggacaggc gctgagggtt ccaaaatgtt tttacggcag cattcttttt accgggcatc
     2161 aggcggctgc gttgccagca gcgccagcgc ctcgcgcaca cgggcacact gcacagcatc
     2221 agcaagcaga taaaaatgct ttcctccgtc ctttgcttca aaaaaagtcc tgccctgatc
     2281 cgtcacggtt acagtacctg ccggcgaaag tgtaaagtag ccccggtcag ggcgcactgc
     2341 ccagagaaca ctcgtcagat cccagcaggg ccgttcatgc ggcgtgggct cgtacatctg
     2401 gtatgcctca ggcagcggat gacggggcgc ataggcgaag tcccgatcga tgcacaccgc
     2461 cgggaaagtg atggcgatac cgatttcaaa accgctgagc accacagggg tgggccattc
     2521 ttccagcagc ctcttcgccg aaggaatatc catgaccaca ttgtactcgc aatgctcggg
     2581 cagtccggga atatcggtga aggcacctgc cataatggaa atgtatttaa cttttcttgc
     2641 cgccagttct tttccgctca gcggtgaaat atcatccgcc tccgaatcca gcagccgcgc
     2701 aagattggtg gaaaaaccca cctgcacaat caccactgaa tgatcctcct gcgctgcaag
     2761 cgtttttctg agaaccgctg tcgcatcggg cgcatcattg ccgctcatga gatcatgcgg
     2821 aaaacgccac tgctccccgt ctttcagccg tgccacgccg gtaaatttgc ttttttccgg
     2881 cgtcgggccg cctttcacga taccaacagg gatatcgccg cgcccgtaga aatggttgat
     2941 ggcatcggca catgccgctg tttcttcttc atcttttgta atggtcacgg caagaagttc
     3001 gcactcaccc cgactctgaa gcgcatgaat cacgcccagc gccagcgcat cgtcaatgtc
     3061 gtttcccaga tccgtatcaa aaatcacctt aaccggcttt tccggcgcag cagataaaag
     3121 atcggatgaa atggcatcgg aagccgtcag aacagcaagc atcattccca gatagagcat
     3181 tcgttttttc ctttctcata cgtctcaggc aggcagtgac gcactttttt cccgcatcat
     3241 tacgataaaa aagcatacca cagccaatac accgctgatc cagacgcccg gactgcccgc
     3301 cgaaacgctt ttgttttctg tcacaaccag atgtatgacc ggagaagtgc gtgtataaaa
     3361 atcgtcccgc acttcacaga agtaaccgcc tgtatcctgc tccgatgcca gcgaaaaaga
     3421 gagactgtct ccggtctcgc ctgtcagcgc ttcgtggatc ccgtcacccg acacccggta
     3481 ccactgatag cgaagcgtgc ctctgccgtg actgacaagc agccgcaatt ccgctgcgtc
     3541 gccttcaggc acctgtacaa tcccgtcgcg cgccggtgat accgaaagca cctgctcatc
     3601 aaagccgaga cgccggtttc tctggctttc ataaagcgac tgaggctcaa gcagatccgc
     3661 agcatcgatc agttccttat aatcccaggg ctctgtgcct gttgtcaggc cgccggtcac
     3721 ctcatggccc agcgttttta ccgcaatatc ttttgtgccg atcacatatc catgcccgaa
     3781 attgtaggaa cgaatctccg ctttatccag gccgcgggta ttccataaag cgcattgtgt
     3841 cgcgctgtgc cctcctcctg tgctgtaatg ccgccgattc cccgctgacc agccatcatt
     3901 aaaaaaacag gagtcgacca ggcatgccat ggaaagcgaa tgatgatact cgctgaaagc
     3961 gcgcaaacgc acctcttctc ccagaacaag ataggtctgc gcactgtcca tactttcaca
     4021 gcgcagccag acaataccgg tggcgccgaa tccccaattc tgcacaaaat tgtgccgacc
     4081 ttttctcgcg acacagtcct gatacagaat ttcattgctc tggcggactt catacaaata
     4141 accgttaccg ccgtcaccgc ggttctgcga attttccatc tgacagtacg ccaccgtcac
     4201 ccgcttggaa ttgttaatcc ggatgccgcc gctctgcaca tgtttttcaa gaggatccac
     4261 gcaggccgga gactgaaagc tccggatccg gtaaatatag ctgtctttca catctgtcat
     4321 ctgaatgatc tgggtctgac gctgcgacca ggcaagatcc cgctcgatgg catcggacac
     4381 ggcaaggttc atgacaccgc agccactcag atagcccggg ttgcgcgtca gcactgcgcc
     4441 gtcacgaagc ttcgccggat agcgcagtgg cacatccagg gtgatcacag gcggattcga
     4501 atccgaatca atggccgtaa tggtgcgctg aaaaaaaggc tgccactcac cgttaaatgc
     4561 ctgccaggta ccggtcatgc cgtgctccgc aacaaactca tccgtaatcg tccagcccac
     4621 cgtcacatca tcacccacac tgaaagcgct gctgtctgtc acacgaatat caaaagacaa
     4681 cgcctcaccg tcttccgcaa gaggcgtgct gtcccgctgc ttgaccgagc ctccgaatat
     4741 aaggtgggca tggcttgaag tgctgtggca ctgcgtgaaa taaacatagg tattgcttgc
     4801 actttcgccg caaagaacaa tattggatgc caggacagcc aaagacccct cacagcgata
     4861 aatacctgca ggaagataaa ccagcccgcc gcctgctgac cgacaagcgt ctatagcggc
     4921 ctgaatcgcc gctgtcgcat cggatctccc ctggttatcc gctccaaatt ccgcaaccac
     4981 atcaaagcgc gggcttatcg tcgcagggtc aggaagattc ttgccgttct gatagcctgc
     5041 atatgaaaaa tcatgaagaa agcgtcccgc accgtcatga aaacccggag accagtccgc
     5101 cgggtacagg gcactgcgct ccgcaaaaga ccgggcgctg cacagcaaca ggcagacaga
     5161 cagaaaaaca gaaaaaactc tcagcatgac agattcttta actgttataa taaggtatga
     5221 taagagggaa aatgaatgca tattctaagc gggtaaaaac gcctgaagaa ctggtattct
     5281 atcatatctt gaagagagac agcatcacct gcgccaaatc cacagttctt cctattttcg
     5341 catatagaag cagcggataa tgcgacgttc ttgaatttca atttttaaat aagccacact
     5401 cataggatag atactgtggt actattcaaa gcgtggcaac aggagataca ccatgtcatt
     5461 ccacttgaaa ctcggcattt tcctcatcgt ctctctcgta ctgctcgtca gcagcaccgg
     5521 ctttggcgat gaccactggg ggccctgccc ggcatctcct gccgtcgtga atccgcccat
     5581 gctgtctccc ttccaatccg ttctggccct gcggggcgaa tgggattttg tagcggatac
     5641 caaccttctc ggacggcatc gcatggggaa agggcccgag tggaacgaac ccgactggag
     5701 ccaggtgcga acccttcacg tgcccggctg ctgggaggca cagggtgtag gtgaaccggg
     5761 cataagtgaa acctgggacc ctctcttcga ccgtatcccc cgcccgatca accacaagta
     5821 catgggtgcc ggccgctaca gaaaacagat tgacattccg aaggactggg acggcaagcg
     5881 cgtgtggctc aaaatcggcg gcgtccgcac cgaagcctgg atctgggtca ataaaaaacg
     5941 ggtcgcccac ctcaacacct tctgcggcac ctacaaatat gacatcaccg atcacgttgt
     6001 accgggcgaa caggtggaaa tcatcgccac ggtgcgcaac gattcaccga gccgcaaagg
     6061 ctgcatggca gcctaccatc gcttcggagg attctaccgg gacatcgaac tggaggcaac
     6121 accgcaaaca cgcattgatg atgtctgggt gcaggggcgt cttgaccgga aaggcgcccg
     6181 ggtacatgtt tcgatccgtc acgagggcaa cagtgacccg ggcacgctgg aagtggcggc
     6241 agccctcgca accctcaatg gcgaacgggt ggcaactttt cagcatccga tcacactgaa
     6301 tgcggacgga atcgccgacc ttgccgtgga aacaacctta atgccttttc atgcctggac
     6361 cccggaaaat ccatttcttt atcttgcgga agtcgtgctg ctgcagaacg gcgttcctgt
     6421 ccacggctgg gttgaacgtt tcggcgtgcg caaactggaa gtgaaaggtg accgctttta
     6481 tctgaacaac gagccttttt tcatgcgcgg ctacggcgat gattacatct acccgctgac
     6541 ccttatctca ccgccggacc gggaggcgca ccgcaaacat ctggatatcg cccgacgtgc
     6601 agggttcaac tatgtacggc accataccca ctgtgagatc ccggaatttt ttgaagcagc
     6661 cgacgaagcc ggaatcctga tccagcccga actgccgtat tatcatgata ttacgacaga
     6721 agccttttct tttgatcccc tccgcgatct gcaggaactc tacaggcatt accggcgcta
     6781 cgtttctttt gcctcctact gcacaggcaa cgaagggcat ctcggaagcc ctgtcgataa
     6841 ggaagtgtat gcctgggcaa agcagaatga tccggaccgc atcatgcagc atcaggacgg
     6901 cggctgcaat acaccggaca atgccgacta tgacacgccc aacagctacg gcggcatggc
     6961 tacctccatt gtgccctgga tgcccggcac ctttgacgcc cttaccatgc ccttcatcgc
     7021 ccacgaatat ctgaacctcg gcattaaaat ggatccgcgc acggaaccgc tctttaccgg
     7081 agtgatcccc gcgccccgct ccatggaaaa ctacgaggca tcgctcgtgc aggcaggtct
     7141 caaccgtacc tggggcgacc gctgtctcag ggccgcacat ggtctgcagg gctattatca
     7201 gaaacagggc atcgaacagg cgcgtctcga tcccgcctgc gacgggtatt cctactggac
     7261 gattattgat gtcatggtca atcaggaaaa tacctataca ggtcagggat ttctcaatgc
     7321 cttctgggaa ccgaaggaag gcgggctgac tcccgaagaa tttttcgcct tcaacgggcc
     7381 taccgtactg cttgcagatc tggacggaat caatgccgtt ctgactgccg gagaatctgt
     7441 tgctgttcct ctatggatat ctcatttcgc tgcggagccg ctgaacgatg cagtgttgac
     7501 ctggacgctg gccgccggag ggaacccgct gctctccggt gaattcgatg ccggcagttt
     7561 ctctcccggt gacgtacgcc aggcaggcac aatcacactg gaaacgccgt ccgttgttac
     7621 acctgtcaaa gcaacacttc aggtcactgt caataaaacc gatatctcca accactggga
     7681 tttctggctt ttccctgcga gacaggaaca gcccggtaat gatatcgccg ctaccgaaga
     7741 tctcttcccc gaaattgcac gacgttatcc cggtgccgca cagctgggca cggcagaagc
     7801 agaaaaagca tccctcgtca tcgcctcgga aaatcatccc gccctcactg acacctctct
     7861 gaccggcaag cgtgttctca tcatcggcgc tgccgaaggc gagccgaata ttaaactggg
     7921 ctggtggtcg ctgggcaatc agctgggcac cgcctttgaa agccaccccc tcttcggcga
     7981 tttccccgta gaagaaagca tctcgccgct ctggttccgg ttgattaaaa aaggaatgcc
     8041 tcttccgctc aatgccgaag aaggacaggt ggaacatatt gtgatcggcg aaggacgcaa
     8101 aaactatttc acctatctct accggcagaa acaggagcac agcctgcccc gcctgcacag
     8161 ccacgggctc gacctgctca acggcacccc cgaagcggac agtctcctcg acaatctgct
     8221 tgactatatg cgaaccgacg ccttcatcca gttttaaacc gggcgttttc acatctctcc
     8281 caacttattt ctcataagtc ggatgagatc cctgcgcctg cttcatcacc tgcctttccc
     8341 gggcagagac ccgctgagaa tgtatcggac acagcgaaga caactcagac agcgtgttca
     8401 tttccctggc tgactcaaag cgtcgccttc gcccgtggtg cttatttttg gggaagcagc
     8461 agaacgaact gtaagctcag tgccctctac tccacttggt gtgcttcaag aaacatagcc
     8521 ttttccagtg ccataaaaaa ttgttggtaa ggcgccggat cttctacggc ggtaatatta
     8581 tactgcgcat tcgcacgaac aagaagttgg gattctccgt gtggccgtac cgacacggtc
     8641 atacgtaagg catagccatc caactttgtc ccgcttactg aaccaagctc agcatctgct
     8701 ttatcaataa caaagctcaa atcttgaagc gtggcgataa cagtacgaag tgttttgggt
     8761 atatcggtag tatcaaacgc acgactttgc atttgtcgta aagcaacctg cgactcagag
     8821 gctgataaaa tttgcgactt tgagtttgtc tggcagccca caaggaagag taatgtgccg
     8881 gtcaggcaaa ttgcatatat acttttttta aatgttatca tgctgaaatc ctttatattt
     8941 catgtgcttc aaggaagact gatttagaaa gtttttcaaa gaactcctga taaatagcga
     9001 tatcttctat cgcttctgtt cttgatattt tcccttcatt gttccagaca actctttgaa
     9061 aagtaacgcg taatttagtg cgttcaccat caagaagact tgtcaccaat gaggcgcgta
     9121 ttttttggtc cttgtcgaca gaagttttca cgccgaaaat agccgcaacc ataaaagatc
     9181 ccacggcctg cccaaattca cgagcatcac ggtcttttga accgaccaaa acaccgagat
     9241 ccatctcaga ttcatccaaa gtaaagccaa ggtcttgcaa aactcccgcg gcagccgcaa
     9301 gcaacacttt ttcctctact gtatcaaaag aacgtgtctg agcctgacga agctgcatgc
     9361 tttcttcttt tagcagaagc gcatctttgg gaatcttggt agactggcat cctgcagcta
     9421 gtgtgattac tatgcagacc atttgaagaa aaagtacctt ttttatcatt ggcttttctc
     9481 ctaaaatttt gaagaatggt atgcgaaatc acgaactaac ccttctgaat caaacttaat
     9541 gattacagtt aaggttttct gtgtcgtgct actggcaccc gaagacgcac tatagctagc
     9601 gcctccacct ccagcaactg aagcccctcc agcaaaaaca agcgcagcta ctccgccgga
     9661 gctactcgag taagcacgtt ctgtagaaat tttgtcgtaa atccagactt cacgccgttc
     9721 ttcatctgtt gtgacaacat tgggcgatcc gagtacagaa gcaacatctg cacccgacat
     9781 tcctacccga atttctttct gcaccgttcc aacggtgagt cgcccagtat tgtcgtcgga
     9841 gactgcggcg cgatgtgatg cggccgatgc gcatcctaaa gctgttaaca agatagcgta
     9901 cgacaagagt gtggcaatgc ttcgaatctt cataacatat ctcccgtgtg gtttaaggtt
     9961 gaattaggaa taggtcttgt tttttgcgat ttttcccgtg ttcgtatatt tgtatttcag
    10021 gctaaataca gaaagaattc ttttcgtgcc cgaccggacg gcgcttttaa aaaggagaag
    10081 gctaaagcgg ttcccccgaa taatcttctt aatttgatat atatacgcct tatgctgctg
    10141 atggttcaga caaaaagact cagttgtact gcgcgctttt atcacccctt gcccctccat
    10201 tgctgtttcc aattcataac gggattgcct ccaagaaaac aactgatcca tattttttca
    10261 tatatacaag atgtttgtca acggttatgt caagaagttt ctgtaaatta gttttcctgg
    10321 tgaacagtgc gttgtt
//
LOCUS       DAOPWB010000110        10266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6576_length_10266_cov_4.982176, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000110 DAOPWB010000000
VERSION     DAOPWB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6576_length_10266_cov_4.982176"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..1200)
                     /locus_tag="PKN92_09870"
     CDS             complement(322..1200)
                     /locus_tag="PKN92_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNZ49142.1"
                     /translation="MTHKKYTRIIFALLFTMIWTAAASAAPLASHVFIISYDGGAPYV
                     MKDCDMPVLKGMLDQGACTWEAQTIFPSVTLIAHTSMMTGMGPAKHKVTWNGDEPDKK
                     LEVTTCFAEARKKGLHTAFFPGKDKLLLIAIPDSMDHAECPGYESDKVAAAAAEYIVA
                     NKPNLTFVHFAETDGLGHKRGWGSDEQKAGFANADKALGVLKEAVEKAGIADDSVFII
                     TADHGGHKNTHGSNKPEDMTIPWILWGKNVKPGHTISEDVTVYDTAATALWLLDVEIP
                     ESWDGKAITNAFNTPE"
     gene            complement(1222..2337)
                     /locus_tag="PKN92_09875"
     CDS             complement(1222..2337)
                     /locus_tag="PKN92_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AP protein"
                     /protein_id="HNZ49143.1"
                     /translation="MKSTRVLIGLLTLALCMAMAFHATAQEKKFKTENIIFVMTDGLR
                     WQEVFQGAEESLLPEAKSGIHPSRAAYWRDTPEERREILMPFTWKVMAKEGQIFGNQN
                     KGSVSQVSNGYNFSYPGFSEVACGYGFRDVDSNNKIYNPHLNVFEFLNNMPAYKDKVG
                     CFGAWELFPWILNRERSGMLVNAGYEPLDTGKSFPEYELLNRMKKETTHYWGGEPFDS
                     ITFETAYAYFKREQPRVLFLALGETDEWPHGGRYDLYLDAARQADNAVRYLWETAQSL
                     DQYRDKTTLIFTTDHGRGYAPLEWKSHGEDVANSENTWMAFLGPDTEPLGEREKVERV
                     TASQLAATMAAFLGEDFRAAAPEAGKPIDDVLGSTNN"
     gene            complement(2585..5179)
                     /locus_tag="PKN92_09880"
     CDS             complement(2585..5179)
                     /locus_tag="PKN92_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49144.1"
                     /translation="MLFPAHTRSLFALLCLTLPLTAPFPAVAAEFSIDADYPGGNILI
                     KKIEGDQVLLAPDLRDTAGWWFYWNFRVTGAAGRTVTFQFDGQSPIGVRGPAISQDGG
                     TTWAWMGAECVTKNSFDYTFSADACEVRFAFTIPYLQSDLDAFLKKHEKSSFLSVTTL
                     CATGKNRSVEKLHIGNLEGDPRFRVLITARHHACEAMANFVLEGLMDAILADTEDGCW
                     FGRNVELTVIPFMDKDGVEDGDQGKNRTPRDHNRDYIGVSVHKETEALRTFVPNWANG
                     KLRVALDLHCPHIRGRHNEDIYIVGSRHEEMWVEEQRFGSLLEEANRDGLLPYSCSNN
                     LPHGKSWNVSRSYSAGLSFDGWASGLDGIQLATAFEIPYANAGGTAVTPENSRNFGSS
                     LAKALRAYLQEKEKVERRTSEYPVFSGGVFPSLTVMAQGKGSTSEAGIGALIPWGNRL
                     WAVGYVAHINGQGLGLYEIRENMTMERHPASITGTYANRMAHWPSGQAFIGPYAIDAD
                     GQVRTIEAFREIRLAATCAHLTDPRNKVYFLGMEGGFWEVDVKSLEAVFLFDLKKELG
                     LQDVLVHFKSAHTAQGRVVVANNNYDEQEYLGLRQAGRLAEWDGSSWNIVERNPFVEV
                     AGTFGNDSYGGDTLYALGWTDSSLVLRVLVKGEWRRYLLPKSSQCWDHAWNTEWMRIR
                     HASTERLMMDAHGTFFELPPFAYGGEIWGIRPVCTHLRVVPDYCHWRGFFVMASDQID
                     HDQGQPQSGLWFGNIDDLWSMGKPSGWGGPWRNTALQAGDCSDPYLMTGYDAKGLHLH
                     CQADEPVRIDVEVDFLGNRQWVPYHSFELTPGQTYRHHEFPAGFSAHWVRLRVNQPCT
                     ATAQFFYN"
     gene            5423..5896
                     /locus_tag="PKN92_09885"
     CDS             5423..5896
                     /locus_tag="PKN92_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNZ49145.1"
                     /translation="MATEKTVAERCRALSLRLAVAESCSGGLIAHRLTNIPGISECFL
                     GGVVSYANSAKTALLGVPAEMIAAKGAVSEEVARAMVTGVCSRLSADVGVAVTGIAGP
                     GGGTVEKPVGTVWIAAACRGSVLAQKHFFTGDRDAIKAQTADAAFLLILKVLESL"
     gene            5893..9411
                     /gene="dnaE"
                     /locus_tag="PKN92_09890"
     CDS             5893..9411
                     /gene="dnaE"
                     /locus_tag="PKN92_09890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNZ49146.1"
                     /translation="MSTPFVHLHVHSHYSLLDGLSKPSELIQRCVDYDMPACALTDHG
                     SLFGLLEFQGAAKQAGIKPILGCEMYIAPGSRKSKSGKSAKEASNHLVLLCENETGYH
                     NLCRLSSIGYLEGWHYRPRIDLEALEEHKEGLILLTACLQGRVPSLLEQGKQEEAART
                     LDTYLGIFGNDHVYIEMMNHGMPQEERINPMLWEMAHQYGLPAVATNDSHYLDESDWQ
                     AHDILLCLQTKKTLDDPNRMRFYNHDFYFRPGDEMAGCFSRWPEAVSNTLLLAERCNA
                     EIPTNLKLIPNYPVPDGKSKEAYLREQVFEGLHKRFGDELPSAVVERVEFELGVISRM
                     QFVDYFLVVWDLLNYARKEGIPVGPGRGSGAGCLVAYALFITDVNPLQYGLLFERFLN
                     PERKTMPDIDIDFCITRREEMIAYSRRQYGTDCVCQIVTFGRMLARNAVHSVARTMGM
                     SPAESKRIAGMIPSEPNIKLQTALEKEPDLKRAVAEEPEVARLWEMALRLEGTISSFS
                     THAAGVVICDHPLTDHIGLFKAAGSDTVVTQVEMKGVGDIGLLKMDILGLRTLTMIDM
                     AVKLVKKNHKIDLDISRLDISDEKTYALLRSGATLGIFQLESAGMRDLAKRIGLESLE
                     EISALLALYRPGPMKLIDSYVENKRAADKIVYDPPVLKELLQETYGIPIYQEQVMQMT
                     QSCGGFSLGKADLVRRAMSKKDVNLLNEQEAEFIAGCEKNGYNKSLARDLWSRIETFS
                     GYGFNKSHSVAYALVAFQTAYLKAHYPVEFMCALLTTESGDPKKTAIYVAECHTMGIA
                     VLPPDINKSDVSFSAEGKAIRFGLGAIKNLGEAPCTAIARNRDENGPYKDIYDFCDRL
                     IDASVNARVIDSLNKAGAFLDTGWNRHQIAAVMEQALQIAQSRLRERESGQTSLFDLA
                     EEDSGFTVYPEQPDLEEWRMEEVLTYEKEMLGLYVSMHPLHNQEGLVRRFSTLDLNDL
                     AEKKDGEEVFLCGIITEAKKIYTKKGDAMAFLVVESLQGPCEVTVFSRTYEEKKDLLV
                     EDTIVACHLKVNFRDDKPTLIAENIVLLEEAEKHFATALHVRLSRAKQEVSTLHKVAR
                     ILGRNSGASDVYLHCELADGGEIIVHATDACRVTLSPELEMEVEEETGLNTVFTSLGM
                     GLPSHTLPRVIEADVPRWRRRKDPNE"
     gene            9482..>10266
                     /locus_tag="PKN92_09895"
     CDS             9482..>10266
                     /locus_tag="PKN92_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49147.1"
                     /translation="MPPFGYAEIVSRKTMRKNMSNRLAPCTGVFLPALVLSVFLFLFG
                     CSTTHYKDKADTEVYAILQDKSEQVPNAETDFSIDPPGEPVLLEQLPKVERDSRELGH
                     GIPDQTGSAIINLEQSVLLAVQRNRSYQNAKEALFLQILALTLERHRFAPIFSDQGKA
                     AVQGDAVEVTHPSEFSAAMGKTGAIISELESLTGSQADLLRAYTAVVNEAGGLAGLDQ
                     PQTDLVHRQRLTGQHSFGVDKLMKGGGRIVASITTSFMNFITG"
BASE COUNT         2424 a         2573 c         2791 g         2478 t
ORIGIN      
        1 catccaggga taactgagaa aaccccataa gcctttttct tcatttgcca tctgcgttat
       61 accttctgct ttagaatggt tcgagagact ctcgactgag cgagtactat gttccttagg
      121 gaatgatagc acattgtcct ggagagcacc aagcgtaaaa gccttgtctt ttcttaaagg
      181 tgtccgacac ggaaaagaaa agcccgggct gtgttcatgg ggggagaact tcagcccggg
      241 cgggaggtgt tttttcgggt gatcagtcgc tgttacgtca ggtgggcagt ccgatatttt
      301 ctacctgctg tcgtcgcctg attattctgg cgtattaaaa gcattggtga tggctttccc
      361 gtcccaggat tccggaattt ccacatccag aagccataat gccgtggcgg ccgtgtcata
      421 aacggtcacg tcttcagaga tggtgtggcc tggcttgaca tttttacccc acagaatcca
      481 gggaatagtc atgtcttcgg gtttattgct tccgtgcgta tttttgtgac cgccatgatc
      541 tgccgtgatg atgaacacac tgtcatcagc gataccggct ttttccacgg cttctttcag
      601 gacgcccaga gctttgtcgg cattggcaaa tcctgctttc tgttcatcag atccccaccc
      661 gcgcttgtgc ccgagtccgt ccgtctcagc gaaatgcaca aaagtcaggt tgggtttgtt
      721 tgcgacaatg tattcggcag cagcggcagc cactttgtct gattcatagc cggggcattc
      781 agcgtgatcc atcgaatcag gaattgcgat caggagaagt ttgtcttttc ccggaaagaa
      841 ggctgtatgg agtccttttt ttcttgcctc cgcaaagcag gtcgtcactt ccagtttttt
      901 gtccggctca tcgccgttcc aggttacctt gtgctttgcc gggcccatgc cggtcatcat
      961 ggaagtgtgt gcaatgaggg tgacactggg aaaaatagtt tgtgcctccc aggtgcaggc
     1021 gccctgatcc agcatgcctt tcagaaccgg catgtcgcag tctttcatga catacggagc
     1081 gcccccatca tagctgataa taaaaacatg gctggcgagg ggcgccgctg aggctgccgc
     1141 tgtccagatc atggtgaaaa gaagagcaaa aataattctg gtgtatttct tgtgtgtcat
     1201 tcgatatctt tcttttgtac cttagttgtt tgtgctgccc agcacgtcat caatgggctt
     1261 gcccgcttca ggggcagccg cccgaaaatc ttcacctaga aaagccgcca tcgtggcagc
     1321 gagctggctg gcagtgacgc gttccacctt ttcgcgttcg cccagaggtt ccgtatcggg
     1381 tcccaggaaa gccatccagg tattttccga gtttgcaaca tcttcaccgt gactcttcca
     1441 ttccagaggt gcatagccgc ggccgtgatc tgtggtgaaa atcagcgtgg ttttgtcgcg
     1501 gtactgatcc agtgactggg ctgtttccca aaggtatcgc acagcattgt cggcctgtct
     1561 tgctgcatca aggtagaggt catagcgtcc gccatgaggc cattcgtctg tttcgcccag
     1621 tgccagaaaa agaacccgcg gctgctcacg tttaaaatag gcgtaagcgg tttcaaaagt
     1681 aatggaatcg aaaggctcgc cgccccagta atgtgttgtt tcttttttca tcctgttgag
     1741 cagttcatat tcgggaaagg actttcctgt atccagcggc tcatagcctg cattgaccag
     1801 catgccgctg cgttcgcggt tgagaatcca ggggaagagt tcccaggccc cgaaacatcc
     1861 gaccttgtct ttgtaggcag gcatattgtt aaggaattca aaaacattga gatgaggatt
     1921 gtaaattttg ttgttgctgt caacatcacg gaagccataa ccgcaggcca cctcgctgaa
     1981 accgggataa gagaagttgt agccgttgga tacctgagag acgctgcctt tattctggtt
     2041 accgaaaatt tgcccttctt ttgccatgac tttccaggtg aagggcatca ggatctcccg
     2101 gcgttcttcc ggtgtgtccc gccagtaggc ggcgcgactg ggatggatgc ctgattttgc
     2161 ttccggcagc agggactctt cagcaccctg aaagacttcc tgccagcgca gaccgtcggt
     2221 cataacaaaa ataatatttt ccgttttaaa tttcttttcc tgtgcagtgg catgaaaagc
     2281 catggccatg cacagtgcca gtgtcagaag accaattaaa acacgcgtac ttttcatagt
     2341 aatccctttc cggatttgaa ggttggggga aaatcctgcg aagactgcaa ggaacaccat
     2401 tacagccgta tcaggaggga gcaggcggaa aaccacccgg gtcggctgaa agtaaaccct
     2461 gaatatttat cctctattga aattatattt atcggttatt ttgctattat ctacggaaaa
     2521 tagcattaaa tcaatgcaac aaaaagggca ggtgttttgt ttcctgctgc ttgttgacgg
     2581 atttttaatt gtaaaagaac tgcgccgtag cggtgcaggg ctgattcaca cggaggcgta
     2641 cccagtgggc ggaaaagccg gcgggaaact cgtgatgccg gtatgtctgc ccgggagtca
     2701 gttcaaaaga gtgatatggt acccattgcc ggttacccag aaaatccact tccacatcga
     2761 tccggaccgg ctcatctgcc tggcagtgga gatgcaaacc cttggcatca tagccggtca
     2821 tcaggtaggg gtctgagcag tctcccgcct gcagcgccgt attccgccac gggccacccc
     2881 agcccgaagg cttgcccatt gaccagaggt catcaatatt gccgaaccag agccctgatt
     2941 ggggctgccc ctggtcatga tcaatctgat ctgatgccat cacaaagaat ccacgccagt
     3001 ggcagtaatc aggaacgacc cgcagatggg tgcagacggg acggatcccc cagatttctc
     3061 cgccgtaggc gaatggaggg agctcaaaaa aggtgccatg ggcatccatc ataagccgtt
     3121 cggtgctggc gtggcggatg cgcatccatt ccgtattcca ggcatgatcc cagcactgac
     3181 tcgatttggg gagcaggtag cggcgccatt cacctttaac cagcacacga agtaccaagg
     3241 aggaatcagt ccagcccagt gcgtaaagtg tgtcgccgcc gtaactgtcg ttgccaaaag
     3301 tgccagcaac ttctacaaaa ggatttctct cgacaatatt ccaggacgag ccatcccatt
     3361 ctgccagacg ccccgcctga cgcaggccca ggtactcctg ctcatcgtaa ttgttattag
     3421 ccacaacaac cctgccctga gcggtgtggg cgcttttgaa atgcaccagc acatcctgaa
     3481 gtcccaattc ttttttcaga tcaaaaagaa agactgcctc cagacttttt acgtcaactt
     3541 cccagaaacc gccttccatc ccgagaaagt aaaccttgtt tctgggatct gtcagatgcg
     3601 cacaggttgc cgcaagccgg atttcccgaa aggcttcgat ggtgcgtacc tgcccgtcag
     3661 catcgatggc ataggggccg ataaatgcct gtccgctcgg ccaatgcgcc atccggttgg
     3721 cgtaggtgcc tgttatggat gcaggatggc gttccatggt catgttttcc cggatttcat
     3781 aaaggcccag cccctgcccg ttaatatggg cgacatagcc cactgcccac aacctgttgc
     3841 cccagggaat cagggcgccg atgcctgctt cagaggtgct gcctttgccc tgtgccatta
     3901 ccgtgagaga cgggaataca ccgccggaga aaacaggata ttcggaggtt cttctctcta
     3961 ctttttcctt ttcctggaga taggcgcgca gtgccttcgc cagggagctg ccgaaattcc
     4021 gggagttttc tggcgttaca gcagtaccgc ccgcattggc gtagggaatc tcaaaggctg
     4081 ttgcgagctg aattccgtcg agtcctgaag cccatccgtc aaaggagagt cccgccgagt
     4141 aggagcgtga aacattccag cttttcccgt gaggcagatt gttgctgcag ctgtagggca
     4201 gaagcccgtc gcggttcgcc tcctcaagca ggctgccgaa acgctgctct tccacccaca
     4261 tttcctcgtg acggctgccg acaatgtaga tatcttcatt gtgcctgccc cgaatgtgcg
     4321 gacagtgcag atccagagca accctgagtt ttccatttgc ccaattgggc acaaaggtgc
     4381 gcagtgcttc ggtttctttg tgcacgctca cgcctatata atctctgttg tggtcgcggg
     4441 gtgtccgatt tttcccctga tcgccgtctt ccacgccatc tttatccatg aagggaataa
     4501 ccgttaattc cacgttacgg ccgaaccagc agccgtcttc cgtatcggcc agtatggcat
     4561 ccatcagccc ttccagtaca aaattagcca tggcttcaca ggcatggtgc ctcgctgtaa
     4621 tcagcacacg aaagcgtgga tcgccctcaa ggttgccgat atggagtttt tcaacgcttc
     4681 ggttttttcc tgtagcgcat aaggtagtaa cggagagaaa actggatttt tcatgctttt
     4741 tcaaaaaagc gtccagatcg ctctgcaggt atggaatggt gaatgcaaaa cgaacttcac
     4801 aggcatcggc agaaaaagta tagtcgaaac tgttttttgt cacacattcc gcacccatcc
     4861 aggcccaggt tgtgccgccg tcctgactga tagcgggccc tcttacgcca atggggcttt
     4921 gcccgtcaaa ctgaaaagtc actgttctgc cggcagcacc tgttaccctg aaattccagt
     4981 aaaaccacca cccggctgtg tcccgaagat ccggtgccaa cagaacctga tcgccctcga
     5041 tcttcttaat caggatattg ccacccggat aatccgcatc aatggagaat tcggcggcaa
     5101 ctgccgggaa gggggctgtc agcgggagag tcagacagag aagggcaaaa agggaacggg
     5161 tatgagcagg gaataacata gaacgcctcc tgacactggt tatatagagt gcctttcact
     5221 gtacccgcag ccatctcttt atttcaagtc attcacgggg gcatctgaga atatttcatt
     5281 ttccataata agaacaatac gaaattcaat gctgtaaaaa gaggggatgg atgtggcaca
     5341 ataactctct ggagtgccct gagcctctga ggtgtacgga agcagggggt ttcccgtaag
     5401 acaaatgatg agggttttta ctatggcgac agagaagaca gttgcagaac gatgccgcgc
     5461 tttatcgctg cgccttgcgg ttgccgaatc ctgctccggc ggattgattg ctcatcggct
     5521 gaccaatatt ccgggaatat ccgagtgttt tctgggaggc gttgtgtcct acgccaatag
     5581 tgcgaaaaca gctctgctcg gcgtcccggc ggaaatgatt gccgctaaag gcgctgtgag
     5641 tgaagaggtg gcacgcgcca tggttacagg ggtgtgctca cgcctgagcg ccgatgtcgg
     5701 cgtcgcggtg accggaatag ccggtcccgg tggcggcaca gttgaaaaac ctgtgggcac
     5761 cgtgtggatt gctgcggcgt gccgcggcag cgtcctggca caaaagcact tttttaccgg
     5821 tgatcgggat gccattaaag cgcagacggc agatgccgct tttttactga tccttaaagt
     5881 actggagtca ttatgagcac accctttgtc catctgcacg ttcattcgca ttacagcctt
     5941 ctggacggtc tgtcaaagcc atcggaactg atacagcggt gtgtcgatta tgacatgcct
     6001 gcctgtgcgc ttacagatca tggttcactt ttcggactgc tggagtttca gggtgccgcc
     6061 aaacaggcgg gcatcaaacc tattcttggc tgcgagatgt atattgcccc cgggtcacgc
     6121 aagagcaaaa gcggaaaaag cgccaaagag gcaagcaatc atctggtgct gctctgcgaa
     6181 aatgagacgg ggtaccacaa cctctgtcgt ctgagctcta tcggctatct ggagggctgg
     6241 cattacaggc cccggattga tcttgaagcg ctggaggagc ataaagaggg gttgatcctg
     6301 ctgactgcat gcctgcaggg cagggttcct tcattgctgg agcagggaaa gcaggaagag
     6361 gcggcgcgca cgctggatac ctatctgggt attttcggaa acgatcatgt atacatcgaa
     6421 atgatgaacc acggcatgcc ccaggaagag cgtatcaatc ccatgctgtg ggaaatggca
     6481 catcagtacg gtcttccggc ggttgccaca aacgatagcc attatcttga tgaaagcgac
     6541 tggcaggcgc atgatatcct tttgtgcctt cagactaaaa aaacgcttga cgacccgaat
     6601 cggatgcgtt tttataacca cgatttttat ttccggccag gcgatgaaat ggcgggctgt
     6661 ttttcccgct ggccggaagc cgtttccaac accctgcttc ttgcggaacg ctgcaatgca
     6721 gagatcccga caaacttgaa actgattccc aattatcctg tgcccgacgg aaaaagtaaa
     6781 gaagcgtatt taagggaaca ggtttttgag ggactgcaca aacgttttgg cgatgaactg
     6841 ccctctgccg tggtggagcg tgtggagttt gagttgggag tcatttcccg gatgcagttt
     6901 gttgattatt ttctggtggt ttgggatctg ctgaattatg cccggaaaga aggcattcct
     6961 gtcgggcctg gtcgcggttc cggggcggga tgtctcgttg cctatgcgct ttttatcacc
     7021 gatgtgaatc cgcttcagta tggcctgctt tttgagcggt ttttaaatcc tgagcggaaa
     7081 accatgcctg atattgatat cgacttctgt attacgcgcc gggaggaaat gatcgcctat
     7141 tcccggcggc aatacggaac cgattgtgta tgccagatag tcactttcgg aaggatgctg
     7201 gcacgcaatg cggtgcacag cgtcgcccga accatgggca tgagtcctgc ggaaagcaag
     7261 cgcattgcgg gcatgattcc cagtgagccg aacataaaac tgcagactgc cctggaaaaa
     7321 gagccggatc tgaaacgggc ggtggcggaa gaacctgagg tggcacgctt gtgggaaatg
     7381 gcacttcggc tggaaggcac catctccagt ttcagtacgc atgcggcggg cgtggtgatt
     7441 tgcgatcacc cgctcaccga tcacatcggc ctttttaagg cagccggatc ggatacagtt
     7501 gtgacccagg tagagatgaa aggcgtaggc gacatcggcc ttttaaaaat ggatattctg
     7561 ggtctgcgta ccctgaccat gatagatatg gccgttaaac tggtgaaaaa gaaccacaaa
     7621 atagacctgg atataagccg ccttgatatc agcgatgaaa aaacctatgc tctcttgcgc
     7681 agcggtgcta cgctgggtat tttccagctg gaaagtgcgg ggatgcgcga tctggcaaaa
     7741 cgcatcggtc ttgagagtct tgaagaaatt agtgccctgc ttgctttata ccggccgggc
     7801 cccatgaaac tgattgattc ttatgtggaa aataagcggg ctgcggataa gatagtctat
     7861 gacccgccgg tactgaagga gctgctgcag gaaacctacg gcattccgat ttatcaggag
     7921 caggtgatgc aaatgaccca gagctgcggc ggtttcagtc tggggaaagc cgatcttgtt
     7981 cgacgggcta tgagcaaaaa agatgtgaat ctgctgaacg agcaggaagc ggagttcatt
     8041 gcaggctgcg aaaagaacgg ttacaacaaa agccttgccc gtgatctctg gtcgcgtata
     8101 gagactttct ccgggtacgg attcaataaa tcccacagtg tggcatatgc tctggtcgct
     8161 tttcagacag cctatctcaa agcacattat cccgttgaat tcatgtgcgc actgcttaca
     8221 acagaatcag gcgacccgaa aaaaaccgct atttatgtgg cagaatgcca tactatgggg
     8281 attgctgttc ttccgccgga catcaacaaa agcgacgtgt ccttttctgc ggagggcaag
     8341 gcgattcgtt tcggtctggg tgccatcaaa aatctgggcg aggcgccctg cactgccatt
     8401 gcccggaatc gtgatgaaaa tggtccgtac aaggatattt atgatttctg tgatcggctg
     8461 atcgatgcgt ccgttaatgc tcgggtgatc gacagtctga acaaagcggg agcgtttctt
     8521 gacacggggt ggaaccggca ccagatcgct gccgttatgg aacaggcatt gcaaatcgca
     8581 cagtcacggc tgcgcgagcg cgaaagcggc cagacatcgc tgtttgatct ggcggaggaa
     8641 gacagcggtt ttactgttta tcctgagcag ccggatctgg aagaatggcg gatggaagag
     8701 gttcttacct acgaaaaaga aatgctgggt ctttatgttt ccatgcatcc gctgcacaat
     8761 caggagggcc ttgtccgtcg attttcaaca ctggatttaa acgatctggc ggaaaaaaag
     8821 gacggggaag aggtgttcct ttgcggcatc attaccgaag caaaaaagat atatacaaaa
     8881 aaaggggatg cgatggcttt tcttgtggtg gaaagccttc agggtccctg tgaggtgacg
     8941 gtgttcagcc gcacctatga agagaaaaaa gacctgctgg tggaggatac aattgtcgcc
     9001 tgccacctca aggtgaactt cagagatgac aagccgaccc tgattgcaga gaacatcgta
     9061 ctgctcgaag aagccgaaaa gcattttgca acagcattgc atgtgcgttt gtcccgggcg
     9121 aaacaggagg tgagcacact ccataaggtg gcgagaattc tgggcagaaa cagcggagcc
     9181 agcgatgtct atctgcattg cgaacttgcc gatgggggag aaattatcgt gcatgccact
     9241 gatgcatgcc gggtgactct gtccccggaa ctggaaatgg aagtggaaga agagacagga
     9301 ctgaatactg tatttacttc tctcgggatg ggacttccgt cgcataccct tccccgtgtc
     9361 atcgaagcgg atgttccgcg ttggcggcgc cgcaaagacc ccaatgaata aagctgtgtc
     9421 ccgggatcgg gaaaccggtc gggcgcaagc ccggtcattt tcagaagaag gagttctgtg
     9481 catgcctcct tttggatatg cagaaatagt gagcagaaag accatgcgga aaaatatgag
     9541 caacaggttg gcgccgtgta ccggtgtttt tctgcctgcc cttgttctga gtgtatttct
     9601 ttttcttttc ggttgcagca ccactcatta caaagacaaa gccgatacgg aagtttatgc
     9661 cattcttcag gacaaaagtg agcaggtgcc caatgccgaa acggattttt ccatagaccc
     9721 gcccggggag cctgttcttc tggagcaatt gcccaaagtg gaaagggaca gccgggaact
     9781 ggggcacggc atacctgatc agacaggaag cgcaattatc aacctggagc agtcggtact
     9841 tctcgcggtt cagcggaatc ggagctacca gaacgccaag gaggcgcttt tccttcagat
     9901 tctggcgctt acgctcgaac ggcatcgatt cgctccgatt ttttctgatc agggaaaagc
     9961 agcggtacag ggcgatgcgg tcgaagtgac ccatccttcc gagttcagtg ccgccatggg
    10021 aaaaacaggc gctatcatca gtgaactgga aagcctgacc ggatcacagg cagatcttct
    10081 ccgcgcctac accgctgtcg tgaatgaggc gggaggactt gccggattgg atcagccgca
    10141 aacggatctc gtacacaggc agcgtctgac cggccagcac agtttcggtg ttgataaact
    10201 gatgaaggga ggcgggcgta ttgtcgcttc cattaccacc agctttatga attttattac
    10261 cggaag
//
LOCUS       DAOPWB010000111        10251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6593_length_10251_cov_5.208023, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000111 DAOPWB010000000
VERSION     DAOPWB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6593_length_10251_cov_5.208023"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PKN92_09900"
     CDS             complement(<1..1368)
                     /locus_tag="PKN92_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49148.1"
                     /translation="MVGQCLESGVIYRAYASCAVESETGYAGGIVGLCNTGWITTCSF
                     RGTVEGEISGGVAGHMENSLCEDRWSNGTISGRRAAGGLIGHATSLDDEPCIKNCYSS
                     ATVEKKLSSGTLGGLVAWAGDEVVAKHSYWCIDTAGTEDSAVGEARTLSEMTRPSAFN
                     TYVDWNFSDIWVEDYLGENDGFPIFSDRTANENYSGGSGTLDNPYLISQVRDWLKLLE
                     SPYDWDKHFLLIADLDFKDASIEPLGDWETNKGFSGNLNGYGHCFENLQITAPLESHA
                     GLFAGLENATIYNLCLKNVRTAAAQSAGTLAGYAIDSDISFCKATGTATAGSHAGGLV
                     GYSLRSRFYRCETSVRVQGYMSAGGMTGYAEQNTQFQYCATTENATALYAGGIAGFVT
                     DSGLFTECRTTGNLFGVLMDGQDPALATHAGGIAGFCMSDIEFTNCHSSGNSTGDRAG
                     GIAGYC"
     gene            complement(1408..1863)
                     /locus_tag="PKN92_09905"
     CDS             complement(1408..1863)
                     /locus_tag="PKN92_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49149.1"
                     /translation="MKHHLSFALLFCVVLSGISADAEVRYSGGAGTLQSPYLISTRDD
                     WIAIAGNIEDYSSYFELTKNIEFPDGLTIEPIAGEGTGAVFQGVLDGRGFALYCPTVD
                     CHGEEFGGLFARVDGGTIKNLTLWDANILNGECSGSLVGQLKGGLISKC"
     gene            complement(2351..3328)
                     /locus_tag="PKN92_09910"
     CDS             complement(2351..3328)
                     /locus_tag="PKN92_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49150.1"
                     /translation="MSEKKNISPPESAVGRRDFMKFMGTAALGAGASGVPGASEAAAP
                     SLPSSFPKVKEITLPITHPDALAVTAEDHLLAGGENLVLHLDRNGAELARYPIDGRVF
                     ALLSLGNTLLLGFRHGVVHFDPATEKQDRWEGFNERSCVTAVTKDENHIFVADAGNRV
                     VLRYDLQGRLINRIGDNKDDGTPGFIVPSPYFGISMDPLGMLWVINPGRHGVESFRPD
                     GSPVSTWYKAGASLDAFCGCCNPIHIAWRSDHSLVTVEKGLNRVKVYAPDFSIRGLVQ
                     GPLEITGLPVNLSPREAPPLTGLAVDSQDRILVLNARDRSISIYEEKNA"
     gene            3724..4944
                     /locus_tag="PKN92_09915"
     CDS             3724..4944
                     /locus_tag="PKN92_09915"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNZ49151.1"
                     /translation="MRVNRRAVLPWVVLLVVAAVHISAVATDGHQLVGLGALQIGTGG
                     AGVASAKDSTWLLLNPASMITLERRLDFSFEVFAPYRYMEPKGPLLMPMANRFAGKMG
                     DDSIFYIPAMGAIFPTAHGALGVGLFAVNGMGVDYKQSRTLIPRLLGQNFDRRTKYGV
                     MKVALGYAHDLGDGWALGATATLDYARFKTDMLTLNFWETKGGNRTDDVFGAGLTLGI
                     YKEWERFSLGAAYTTPQWMESFSKYKDLLPLPLDIPQSVQAGFAWRITPELEWVADYK
                     FLDWSGVRQIGKPPIKGGFGWKDQHVFKTGLTWQVHPRWTVRAGTSLANSPIDSKIAF
                     ANALFPAIVKSHLTAGVSFALTDQSDIHFAYEHAFANTITDSGKGDLFSFAGKGTRIH
                     LRENTFTLQYSYKF"
     gene            5067..5660
                     /locus_tag="PKN92_09920"
     CDS             5067..5660
                     /locus_tag="PKN92_09920"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ49152.1"
                     /translation="MSFSQPPLPYDLGALNPFVSEEQMSFHYGKHHLAYFNNLNGLVD
                     GKPEADMALLDVVKQSEGGVFNNSAQAWNHSFFWHCMSPGGGGAPAGDIASRITRDFG
                     SFDVFKEQFSSAAAKLFGSGWAWLAADASGKLEIMPLSNADTPVRHGKTAILTLDVWE
                     HAYYIDYRNLRPKFIEGFWDVVNWDFANKNLVDPFKE"
     gene            complement(5775..6509)
                     /locus_tag="PKN92_09925"
     CDS             complement(5775..6509)
                     /locus_tag="PKN92_09925"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNZ49153.1"
                     /translation="MSKQCALLLVTVLFFGFAGFHADAEPLRVVVVTGGHGYEKEAFE
                     DIFIQNTDIACTFYKLQDDSELFEEISDWHYDVIVLYNMSRRISEKRQQNLLQLLDQG
                     VGLVVLHHAIAAFSDWKEYRKIIGARYWLEDMEEDGIAHSASQWKEGVDMQFHVEDPG
                     HPVTRGIEDFTLHDETYKGYDLEPDNHLLLSCSTEGSQQEVAWTRQYLNARICYIQSG
                     HGAESYASPVYRKLVAQAIAWAGSRD"
     gene            complement(6541..8328)
                     /locus_tag="PKN92_09930"
     CDS             complement(6541..8328)
                     /locus_tag="PKN92_09930"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNZ49154.1"
                     /translation="MIKSSIRFYALCLTALLIAGCAGDSRTELAPPDRSGKSEVETAP
                     AVKPEKAAQQAEKAAARKALPQGKLNIRCQNVLGELLPARVDLLCYEDGFEDRINVPD
                     GVLSISYAIGSYRAYIHVLDNGVPVLVEAEELTITETKPGELRVDLLEGAGGMLPLRG
                     FDSDGDLALDRVELKIGTDPHNAGSIPGKTEFFYDPRVFNKTTQWYCGDLHVHSNYGV
                     GTESVAALVQRAEKAGLDFFAITDRNTLAASEDPAFKSDRMVLLPAMEWGTDEQGVAL
                     VYGPRTRPEMPSTVMAAQAECLRIQAQGGAFVVAHPCFPGKPWQWGLSFVNGIEVWFR
                     NWREVPPMGLPQLGAAAQERKDGRLLHSIAAAVAAVDLGDLSANGQANLFYDYEQVRG
                     LMAGITGGSGSGSPKVPLGRPVTYVHARELSVAGILEGIRLGRTYVSSGTNGPRILFV
                     ADVLSDGKVDVGIGGVVPLNAEVTFEVVVHNAVGKKLQVIENGRPIRTIPINAQDTGI
                     RFKRFPTQDAAYRVRVISPADPKSEGFGPLEVHALTSPIYARDISAELLWRNPNFDPD
                     KSWVRIQNGDLTDVDVQLPDQPPQPVQGW"
     gene            complement(8338..9843)
                     /locus_tag="PKN92_09935"
     CDS             complement(8338..9843)
                     /locus_tag="PKN92_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNZ49155.1"
                     /translation="MANQGKSWVCLVCGYVHHGDEPPECCPVCGAPASDFELQAPTPA
                     PAPTAPVTRWRCLVCGYEHDGTEPPDACPVCGVSSDDFEACTAETADAAVSSDGSRVV
                     ILGAGIAGVSAAESLRNASPGVEITLVSNEARPPYYRLNLTRRLAGEIGDEALPIHPL
                     SWYTDRNIRLLEGKEATEIRVEKREVALDDGTALPYDRLILTCGAHPFIPPVPGADQE
                     GVFSIRRLEDVHALEALLRPGSPCVCIGGGILGLETAGALAKRGIKVTLLENYGYLLP
                     RQLNREASALLSEAVTRLGIQICSQAAAAAITGNGRVEKVVLEDGTELPCEAVCITTG
                     IRSNTHLARRAGLAVRQGIVVDNHLQTSSPGIYAAGDCAEYAGSVYGLWEPAQYQGSI
                     AGLNAAGIATEFGGIARMTTLKVLGLKMFSLGTINPEDGSYTEISEKKDGCYRCYLFR
                     DNKLAGAILVGDTSLATAAARILREGTDCSAAGLPASVHTFESFLRSHLQG"
BASE COUNT         2294 a         2861 c         2659 g         2437 t
ORIGIN      
        1 gcaatagccg gcgattcctc cggcgcggtc gccggtgctg ttgccggacg aatggcagtt
       61 tgtaaattca atgtcgctca tacaaaatcc tgcgattccg cctgcgtggg tggctagtgc
      121 aggatcctgt ccgtccatca agacgccgaa caaattacca gtagtgcggc attcggtaaa
      181 aaggccggag tcagtaacaa aacccgcaat tcccccggca taaagagcag ttgcattctc
      241 agtggtggcg cagtactgaa actgagtgtt ctgctctgca tagcctgtca tgccgccggc
      301 tgacatgtat ccttgaacgc gcaccgacgt ctcgcatctg taaaaccggc ttcgcagact
      361 atacccgacc agtccgcctg catgagaacc tgctgtagcc gtaccggtcg ctttacaaaa
      421 actgatatca ctatcaatgg catagccggc gagggtgcct gcgctctgtg ctgccgctgt
      481 tcggacgttt ttaaggcaca ggttgtaaat tgtggcattc tccaggccgg caaagagtcc
      541 cgcatgggat tccaaaggag cggtgatctg gagattttca aaacagtgcc catatccgtt
      601 cagattgccg ctgaaaccct tatttgtttc ccaatcgccc agaggttcta tagaagcatc
      661 tttgaaatcc agatctgcaa tcaataaaaa atgtttatcc cagtcgtacg gtgattcaag
      721 cagcttcagc caatctctca cctgactgat cagataagga ttgtctaagg tgccggaacc
      781 gccactgtaa ttctcgttcg cagtcctatc cgaaaaaata gggaatccat cgttttctcc
      841 aaggtagtcc tccacccaga tatctgaaaa gttccagtcg acgtaggtgt tgaaagcaga
      901 gggacgcgtc atctctgaca gggtgcgcgc ctctccgacg gcgctgtctt cagtcccggc
      961 agtatctatg caccaataac tatgcttggc aactacttca tcaccggccc aggcgaccag
     1021 tccgcccagc gttccggagg acaatttttt ttccacagtg gcgcttgagt aacagttttt
     1081 aatgcagggt tcatcatcta agctggtggc atgcccgatg agcccgcccg ctgcccgccg
     1141 accggaaatg gtcccgtttg accagcggtc ctcgcataaa gagttttcca tatgccctgc
     1201 cacaccgccg ctgatttccc cttcgactgt tccgcgaaag ctgcaagtgg taatccagcc
     1261 tgtgttacaa agtccgacaa taccgcctgc ataccctgtt tcagattcaa cggcacaaga
     1321 ggcgtacgct ctgtaaatga caccgctttc caggcactga cccaccacgc caccggtttg
     1381 cgtcttgcct tttacggtgc tcctgaccta gcatttcgaa atgagaccgc ccttgagttg
     1441 accgaccaga ctgccgctgc actctccatt caatatattg gcgtcccata gggtcaaatt
     1501 tttaatggtg ccgccatcga cacgggcaaa gaggccgcca aactcttctc cgtggcaatc
     1561 gacagttggg cagtacaatg caaacccacg accgtccagg acgccttgaa agactgctcc
     1621 cgtcccctcg cctgcgatcg gttcgatggt taaaccgtca gggaattcta tgtttttggt
     1681 gagctcgaaa taactgctgt agtcttcaat attcccggct atagcgatcc agtcatcccg
     1741 cgtactgatc agatagggag actgcagtgt cccggcgccg ccgctgtagc gcacttccgc
     1801 atctgctgag atgccggaca aaacaacaca gaacaacagg gcaaacgaga gatggtgttt
     1861 catgaaaaaa ttcctttgtg ctggctaacg gacgtaatcc gacatgatga cagcaattta
     1921 caaggaccca cgagattacg gctgtgtaat tgccataacc gatgaatgaa actttacaaa
     1981 ttgtctacat gaaaaatcaa attatatgct aatactattc tttgtgcgtg atctaatctt
     2041 acaacataaa atataaaatg ccagtattgc tgaggtttaa caatactggc attgaaataa
     2101 atagcattac agggtcggat cagatgacat ccaaccattg cgtctatcgt tgcttgcgaa
     2161 gttaatggaa gacctgcgct tccgggaaga taataggtac gggatgtacg ccctgctatt
     2221 ccgtcagcgc aacgcccatg aagaatagaa agagcagcgt ataaaacagc ataaaaacaa
     2281 aagcgacttc gtccatttca atactccttt gttgtgcctg tgagcgccct gccctggcgc
     2341 actctgagtg tcaggcgttt ttttcctcat aaatgctgat gctccgatcc cgggcattca
     2401 gcaccagaat acgatcctgt gaatcaacgg caaggccggt gagaggcggt gcttcccggg
     2461 gcgaaaggtt cacaggcagc ccggtgattt ccagcggacc ctgaaccaga ccgcggatgg
     2521 aaaagtcggg tgcatagact ttgacccgat taagtccctt ttcaacggta accagagaat
     2581 gatcactgcg ccacgcaata tgaatgggat tgcagcagcc gcaaaaggca tcgagtgacg
     2641 cgcctgcttt ataccaggtg ctgaccgggc tgccatccgg acggaaactt tccacaccgt
     2701 gtctgcccgg attaatgacc cacagcatgc ccagcgggtc cattgatatg ccgaaatagg
     2761 ggctcggcac gataaatccc ggcgttccgt cgtctttgtt gtctccgatg cggtttatca
     2821 gcctgccctg cagatcataa cgcagcacca cccgattgcc tgcatccgct acaaaaatat
     2881 gattttcatc tttggtcact gctgttacgc agctgcgttc attaaaacct tcccatctgt
     2941 cctgcttttc tgttgccggg tcgaaatgaa cgacgccgtg ccggaagccc agcagaagcg
     3001 tgttgcccaa agaaagcagg gcaaaaacac gaccatcaat cggataacgg gcaagctctg
     3061 cgccgttccg gtccagatga agcacaagat tttcgccgcc tgccagcagg tgatcttctg
     3121 ccgtgacagc cagcgcgtcg gggtgggtta taggcagagt gatttctttt acttttggaa
     3181 aagaagaggg gagagaaggt gccgctgctt ctgaagcacc gggaacgcct gacgctccgg
     3241 cacccagcgc cgccgtaccc atgaacttca tgaagtcgcg tcgaccgaca gcggattcag
     3301 ggggtgatat gtttttcttt tcgctcatct tgtcatcaat ccttcaggaa atcagcggta
     3361 cattcatctt gtgaagggct ttctttatag tgtaccacag atttggagcg gcattgataa
     3421 aggcgtcctg gggcttgtgt cgctgtactg gaaatagcgt gcgatttccg gtcttttttg
     3481 attgaccttc gggtgccggc agtggtagca ttatgccgca gccgcatgca tattttcttg
     3541 ggagggttga atgttgacta aatcaataga gatatggtac attacccccg ggcttgggtt
     3601 tggatctacg ctccggcagc tgaatcaggg ataaaactgg atctgctcag acgctgccgc
     3661 ctcttgtttt tatgtcagac acgcccgtaa tggatcggga gaaagaagga gaattttatg
     3721 caaatgcgtg tgaatcgaag ggctgtattg ccctgggtag tgcttcttgt tgtggcggcg
     3781 gtgcatattt ccgctgttgc tacagacggg catcagctgg tgggtcttgg tgcgcttcag
     3841 atcggtacag gcggtgccgg tgttgccagt gccaaagaca gcacctggct gcttttgaac
     3901 cctgccagta tgattacgct ggagcggcgt ctcgacttca gttttgaagt cttcgcaccc
     3961 taccggtata tggagcccaa agggcctctt ctgatgccca tggcaaaccg ttttgccggg
     4021 aaaatgggag atgacagcat tttctacatt cctgctatgg gcgctatttt ccccacagcc
     4081 catggagcgc ttggagtggg actctttgcc gtgaatggaa tgggcgttga ttacaagcag
     4141 tcccgtaccc ttattccacg gcttctcggt cagaatttcg accggcgtac caaatacggt
     4201 gtgatgaagg tggcgctggg ttacgcacat gatcttggtg acggctgggc gctcggtgct
     4261 acggcaacac tggactatgc ccgcttcaaa acggatatgc tgacgcttaa cttctgggaa
     4321 acgaagggcg gcaaccgcac cgatgatgtt tttggtgccg ggctgactct gggtatttac
     4381 aaggaatggg agcgcttcag tcttggtgcc gcttatacga cacctcaatg gatggaaagt
     4441 ttcagcaaat acaaggacct gctgccgctg ccgctggata ttccccagtc ggttcaggct
     4501 ggttttgcct ggcgcattac cccggaactc gaatgggtag cggactacaa gtttctcgac
     4561 tggtcgggtg tccgtcaaat tggcaaaccg cccatcaaag gcgggttcgg ctggaaagat
     4621 cagcatgttt tcaagacagg tctcacctgg caggtacatc cccgctggac agtgcgcgcc
     4681 ggcacttccc ttgccaattc gcctattgac agtaaaattg ccttcgccaa tgcgcttttc
     4741 cctgccattg tcaagtcaca tctgactgcc ggtgtatcct ttgccctgac ggatcagtcc
     4801 gatattcatt ttgcctacga acatgccttc gccaacacca ttaccgacag cggcaaaggc
     4861 gatcttttct cctttgcggg gaaaggcacg cgcattcacc taagagaaaa cactttcaca
     4921 ctgcagtaca gctataaatt ctgatagcag ctgcattttg tccttttgaa ccgcccggaa
     4981 ttattcctgt tccgggcgtg ttctttaaat gctgtttcat gcgtacagaa aacaatggtt
     5041 acaacaagaa caagggaggg ctttttatga gcttttcaca acccccgctg ccctatgatc
     5101 tgggcgcact gaaccccttt gtgtcggaag aacagatgtc cttccactac ggcaagcacc
     5161 atcttgccta tttcaacaat ctgaacgggc tcgtcgacgg gaaacccgaa gcggatatgg
     5221 cgctgctgga tgtggtgaaa cagtccgaag gcggtgtatt caacaactct gctcaggcgt
     5281 ggaatcacag ctttttctgg cactgcatgt ctccgggcgg tggcggtgcc cctgccggtg
     5341 atatcgcttc ccgtattacc cgtgatttcg gttcttttga tgtctttaag gaacagttct
     5401 cctcggcagc cgccaaacta ttcggctcag gctgggcatg gctcgcagcc gatgcttccg
     5461 gcaaactgga aatcatgccg ttgagcaatg cggacacgcc ggtacgccac ggcaagacgg
     5521 ctattctgac gctggacgtg tgggagcacg cttactacat tgactaccgc aacctgcgtc
     5581 ccaaatttat tgaaggattc tgggatgtgg tcaactggga ttttgccaat aagaatctgg
     5641 tggatccttt taaagaataa aaggtatcca cggcagccct gatttgctgt acttcagccg
     5701 gaagctttcc ttaaccgtag gcttccggct ttttgtgtct gcttgtccgg cagaaaaaag
     5761 aggagagtgg ggtatcagtc ccggctgccc gcccaggcga tggcctgtgc caccagtttt
     5821 cggtacacgg gacttgcata gctttctgcg ccgtggccgg attgaatata acagatccgg
     5881 gcattgaggt attgccgtgt ccacgccact tcctgctgac tgccttccgt gctgcagctc
     5941 agcagcagat ggttatccgg ctcgaggtca tagcccttat acgtctcatc gtggagcgtg
     6001 aaatcttcaa ttccccgggt gacggggtgt cccggatctt ccacgtgaaa ctgcatgtcc
     6061 acgccttcct tccactggct agcagaatgg gcgatcccgt cttcttccat gtcttccagc
     6121 cagtaccgtg cgccgataat tttccggtac tctttccagt cgctgaaggc ggcaatggcg
     6181 tggtgcagta ccaccagccc gaccccctga tccagcagct gcagcagatt ctgctgtcgt
     6241 ttttcagaga tacggcggct catgttgtac agcacaatca catcatagtg ccagtcgctg
     6301 atttcttcga aaagctcact gtcatcctgc agtttgtaga aagtgcaggc aatgtccgtg
     6361 ttctgaatga atatgtcttc gaaagcctct ttttcgtaac cgtgtccgcc tgttaccaca
     6421 acgacccgca gcggttcggc atcggcatga aatcctgcaa aaccgaaaaa cagcacagtc
     6481 acaagcaaca aggcacattg tttagacata tttatatcct ttttttgggt tatgtaaaaa
     6541 tcaccagccc tgtaccggct gcggcggttg atcagggagc tgcacgtcca catcggtcag
     6601 atcgccgttc tggatgcgaa cccaggactt gtccgggtca aagttggggt tgcgccacaa
     6661 gagttccgct gatatatcgc gggcatagat cgggctggtc agagcatgca cttccagcgg
     6721 gccgaagccc tcgcttttcg gatcggcggg gctgatcacg cgcacccggt aagcggcgtc
     6781 ctgtgtggga aagcgtttaa agcggatgcc tgtatcctga gcattgatgg gaatcgtgcg
     6841 gataggcctt ccgttttcaa tgacctgcag ttttttccct actgcattgt gcacaaccac
     6901 ttcaaaagtc acttctgcgt tgagcggtac cacgccgccg atgcctacgt ccactttccc
     6961 gtcactcagc acatcggcaa caaataggat acggggaccg ttggtgccgc tggagacata
     7021 ggtgcggccg agacggattc cttccagaat cccggcgaca gacagttccc gcgcatggac
     7081 gtaggtgacc ggtctgccca gcggcacttt cgggctgccg ctgccgctgc cgcccgtgat
     7141 ccctgccatg agcccgcgca cctgctcata atcataaaaa agattcgcct gaccgttggc
     7201 agaaagatcg cccagatcta ccgcagcgac tgccgctgca atggaatgaa gcagtctgcc
     7261 gtcttttcgt tcctgtgccg ctgcacccaa ctgcggcagc cccataggcg ggacctcgcg
     7321 ccagttacgg aaccacactt caatgccgtt gacaaagctg agtccccatt gccagggctt
     7381 gcccgggaaa cagggatggg caaccacgaa agcgccgccc tgtgcctgaa tgcgcaggca
     7441 ttccgcctgc gccgccatga ctgttgaagg catttccgga cgggtgcgcg gaccgtagac
     7501 cagcgccacg ccctgctcgt ccgtgcccca ttccatggca ggaagcagca ccatccgatc
     7561 cgatttgaaa gcggggtctt ccgaagcggc aagggtattg cggtcggtga tagcgaaaaa
     7621 gtcgagtcct gctttttccg cacgctgcac cagtgccgcc acgctttccg tgcccacacc
     7681 ataattggaa tgtacatgca aatcgccaca gtaccattgg gttgttttgt tgaaaacccg
     7741 ggggtcataa aagaattcag tttttccggg aatactgccc gcattatgtg gatctgttcc
     7801 gattttcagt tcgacccggt ccagtgccag gtcgccgtca ctgtcgaagc cccgaagggg
     7861 gagcatgccg ccggcccctt ccagcagatc gacccggagt tctcctggtt ttgtttctgt
     7921 aatcgtcagc tcttccgctt ccaccagaac cggcacaccg ttgtccagca catggatata
     7981 cgcccggtat gagccgatag cgtaactgat gctcagtacc ccgtcgggta cgttgatccg
     8041 gtcttcaaat ccgtcttcat agcagagcag atccacccgg gcaggcaaca gttcgcccag
     8101 cacgttctgg cagcggatat tcagtttgcc ctggggcaat gctttccgcg ccgctgcttt
     8161 ttctgcctgc tgtgccgctt tttcaggctt cactgccggg gcagtttcca cctcactttt
     8221 tccggaacgg tcgggcgggg caagttcggt tcggctgtct ccggcacatc cggcaatcag
     8281 cagcgcagta aggcaaagag cataaaagcg tatggagctt tttatcattg atgtaaccta
     8341 tccctgcaga tgactgcgca aaaatgattc aaatgtatgt acagaagcgg gcagtcctgc
     8401 tgcggagcag tccgtaccct cacggagaat acgtgccgcc gctgtcgcaa gtgacgtgtc
     8461 gcccacaaga atggcgcctg caagtttgtt gtcccggaag agatagcaac ggtagcagcc
     8521 gtctttcttt tcagaaatct ccgtataact gccgtcttca ggattgatcg tgccgagact
     8581 gaacatcttc agcccaagca ctttcagtgt tgtcattcgg gcgatgccgc cgaactccgt
     8641 cgcaatccct gccgcattca gtccggcaat gctgccctga tactgagcgg gctcccaaag
     8701 accgtataca ctgcccgcat attcggcaca gtcaccggcg gcgtaaatgc ccggcgacga
     8761 agtctgcaga tgattatcga caacaatgcc ctgccgcacc gccagaccgg cgcggcgtgc
     8821 gaggtgcgta ttggagcgga tgccggttgt aatacatacc gcttcacagg gcagctcggt
     8881 gccgtcttcc agtaccactt tttctacccg gccattgccg gtaatggcgg cggctgccgc
     8941 ctgagagcag atctgaatgc ccagccgggt caccgcttcc gaaaggagtg ctgatgcctc
     9001 acggttcagc tggcggggca gcagataacc gtagttttcc agcagtgtta ctttgatgcc
     9061 ccgtttcgcc agagcgcccg ctgtttccag gccaagaatg ccgccgccga tgcatacgca
     9121 gggcgaaccg ggccggagca aagcttccag tgcgtgcacg tcttccaacc ggcggatgct
     9181 gaaaacacct tcctgatcgg cacccggcac gggcgggatg aaaggatggg cgccgcaggt
     9241 cagaatcagc cggtcataag gaagggcagt gccgtcatcc agtgcaactt cccgtttttc
     9301 cacgcgtatt tctgttgcct cttttccttc aagaaggcgt atatttcgat ctgtatacca
     9361 ggaaagagga tgaatcggaa gggcttcatc tccaatttct ccggcaagac ggcgtgtcag
     9421 gttcagacgg taataaggag gccgggcttc gttgctcaca agcgtaattt ccacacctgg
     9481 tgatgcgttg cgcagtgatt cagctgcgct gactccggca ataccggcac cgagaatcac
     9541 cacccggctg ccgtcactgc tgacagcagc gtcagctgtt tccgcagtac aggcttcaaa
     9601 gtcatcggaa gagacgccac agacggggca tgcatcaggc ggttccgttc cgtcatgctc
     9661 atagccgcaa acgagacagc gccatcgtgt gacaggtgct gttggtgccg gagcaggcgt
     9721 cggtgcctgc agttcaaaat ccgaggctgg tgcgccgcat acaggacagc attccggagg
     9781 ttcatcgccg tgatgcacat aaccgcagac aagacatacc caggacttcc cttgattcgc
     9841 catcgattag cctttcctgt ttgaaatcat ccaaacagat gattttcttc ccatgtaatc
     9901 ataccattta agtgtatcac ggtcgcaagg gacatacgca aggggccgaa tcaatccact
     9961 tgaaatctta gcgtaaatac agggtaattc agtacagcac ccggcgccac ccctctgcgg
    10021 cgctacccgc tcaccgcatc gctcctgaac cccccgccat tcctgttctt cacctgcgtc
    10081 cttccagctc ctcccctccg tcattcctgc gcaggcagga atcttaattc gggtatggac
    10141 gtcttgattc gcatatcgcc ctgatccaca tcaaagactg caaggcacat aaatacaaaa
    10201 taatccttac tttcaaagat tttcacgcgt agttgacaac aacggcacga c
//
LOCUS       DAOPWB010000112        10128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6691_length_10128_cov_5.427380, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000112 DAOPWB010000000
VERSION     DAOPWB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 10128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10128
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6691_length_10128_cov_5.427380"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            598..1443
                     /locus_tag="PKN92_09940"
     CDS             598..1443
                     /locus_tag="PKN92_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ49156.1"
                     /translation="MFYSGISDEAGQAIAVQIKAHQELGWNYMELRLVDGVNLTQISD
                     AAFDDVYKAVTEADMKVSCFGSAIANWARPITCPAEIDRDDLKRAIPRMHRFHTPFIR
                     VMSYPNDAEHPVEETAWRREAIKRMKELARIAEDNGITLVHENCSGWGGLSAENSNIL
                     LGEVDSPALKVVFDTGNPVTYGQDAWEYYKTVRKDIVYVHIKDARKIDGVDHYCLCGD
                     GDGYVREIVGDLLKSGYDGGLSIEPHLAAVIHTGQKADNDEQLYQSYTDYGRRLMEIV
                     AEVRA"
     gene            1469..2440
                     /gene="pfkA"
                     /locus_tag="PKN92_09945"
     CDS             1469..2440
                     /gene="pfkA"
                     /locus_tag="PKN92_09945"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNZ49157.1"
                     /translation="MKRISVMTSGGDAPGMNAAIRAVVRTAVAKEVEIYGVERGFQGL
                     IEGMLRPLNARSVSGIIHHGGTILRTARSAAFREEEGLSRAVRSLEKNGIEGLVVIGG
                     DGSYRGAHALWKHCGIPCVGIPGTIDNDIGGTQYTIGFDTALNVAMEAIDRLRDTAAS
                     HDRTFFVEVMGRHNGFIGMCSGLAGGAEEVLVPEQPFDLPMLVETLRAGVARGKTSMI
                     VIVAEGGDGGGAYEVSQKVAEASEFKDSRIAVIGHMQRGGTPTAFDRILASRMGVSAV
                     ENLLAGASGMMIAAVNNAMVPRPFEDAWNTRNLFDPEMLQVLHTLGV"
     gene            2493..3596
                     /locus_tag="PKN92_09950"
     CDS             2493..3596
                     /locus_tag="PKN92_09950"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNZ49158.1"
                     /translation="MKLSDRYILGKIWPPALLAAFIISFVVVGGSVRSEMRAVTDSVP
                     ISQMAVLDILRLGLYVLPTLWGYIFPITYLMGVMYVFSRLAQSNELTALKAAGISLRR
                     VVMPVVVLGLLLSVAAFFIADRMQPWAYERLVRLISQELPMRMTLESLPAGVMHEVGD
                     WRVYIAEREDNGDLRGIVILQPNDDGANAFYADSARLVMKKGSLHLELRDGYFIPSDP
                     RQHFMFKSLVKEAPMPKMLKRVLSGEGMTLSSLLSEEKRLARRFDETGALPVGVELRN
                     VRQEVKNRLAFPLMCLAVSFVGAPIGARSKKAGQSYAFSIGLSIIGAYFILRKLVELE
                     ALLPLALTVSLGQMPNLLLCLLGIILLWRVDRV"
     gene            3593..4681
                     /locus_tag="PKN92_09955"
     CDS             3593..4681
                     /locus_tag="PKN92_09955"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNZ49159.1"
                     /translation="MNVLDRYLAGRLLSVFAKVVLSLVLLVCLIDLVVTRQSAIARYQ
                     VPWLVVLQYYLTFAPTILFEYHAAAVSLLVAALIVLGRAAQDNEILALLAGGVSLRRI
                     VRAPVAVAFLIALLVFAVQESYGVHASKLHRHIQSRFFSKITDRTRHGVSWTNLGDGW
                     TCHILKFNTRANSGQDVYLHAFRGKRLEEIRANRIYWDEEKSKWLLEDGRRAIFDQDK
                     EWEILSSRITQEEAPFQEPPEKLFALSVSAQEKSAGDLSRDIEEARSMGVPVREHLVN
                     YHVKFAQPALCFVIIWLAIPFAIRLRKGGIMMSFGVSAMIALVYLVLFAVGVGLGYMG
                     TLPPPAAAWAANALFFIVGLLLFRRVPT"
     gene            4848..6557
                     /locus_tag="PKN92_09960"
     CDS             4848..6557
                     /locus_tag="PKN92_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49160.1"
                     /translation="MADDKQFDNENPEFEHEMNAGKFGDDEGLGNLPPLSDLDSQGTY
                     DSDLDLPPLGGFESQDTSVGGGAPDLLGQTPSGTPFTSDFDASAFNLAGDSGPGGHPS
                     GPLIGGFQDLAADSDFHPETPDIGSGPDATLDTPIWDDSAFGGSGGDLFTPGASENVT
                     QATPAPAFDDFGDAGSFGDFGGTPMPGMAGGTGMDDFGAVPVGAGAPSVSGAQPKARQ
                     GAGALLVIFIGLVSLGVGMIASQPLSTYLTFVPGNPLVMEMQTKDATIANLNRQINTL
                     QQLQATPDRPADISPERVAELQNEILVRTQELDAVKGQLDSTRATLSTEQAELTSVER
                     ELRERNDEVIAAREFLEELRSETAIVQARQRGLIAEVDRLTGYVGELDEANARRIATK
                     EALEHNIDRLVIQVKESIPLTPEKYDYATRLAQVEALRDKAAQAGWVSPELQNEYTSL
                     YLKELELARANDYFFACVPVTDRFGHTSMCWAECLMRGNWAVYYRSLDGKGIGSFENA
                     GSTETPRWEFKQNLSEDTRLAIEAHIVSARIPDFEGHLQRLAERELKAQKDTPWQRVY
                     SSM"
     gene            6702..9212
                     /locus_tag="PKN92_09965"
     CDS             6702..9212
                     /locus_tag="PKN92_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNZ49161.1"
                     /translation="MMPETKAFCVNKAKATAYLVVCLIMAFTVFQANADDTALLMDKQ
                     FFDLGLQAIPSSDGSDCAIQLIWIDLEQLLQENGFPPQPLFVYRSEIAYPGDDGSGGD
                     VVYAGSTAQKGLVDSDVAVGVEYYYTLAVGTYGTQYFLSDTARGSCTGSNLGLRASAL
                     HDIDGKCYIALTWQSVGEPVSLYRSTLGYSSPPDSQLVFGGSKEDNIWLDENVTDGLQ
                     YFYTLVAGQDIIDPKAVSFASAVACDDPMPVYTEVFNNTNPFDLAYSQIIFRPVKEDP
                     HHLTDDLSDMSYADYEVTVLNNVTELPVPRSDHSGFGRDLALGENMQFPVQIGGEKAA
                     SVFFFGKRYSTLTVSANGYISFQEQFGTAPFKETTMVEEHFSEPRIAFFNSALMPQLG
                     GTVWVRLFDDSIVFTFQNVTIKAPTGMLNPDRITAQVEVFFSGHIRMTYLEATMHTGL
                     IGISDGRGVPAEPSGAYPALGLSGAHNWVPFDSLPEVPQRISLLPIAWPAVTAGERIS
                     FIADMELPPGTGSVPAFYGLWSGEGAAPFTDRGDGTGLFDWTPAITLSGFYKVRVVAL
                     LGAERVFQDVILTVRSVDPRALLPRALKLQLSSREGDADPEDDHAVYAGEPLFASYTY
                     EHPLAKENPALYGEGATRIYWYKNGQIVTAYTDRSSVPSGVTRFGDEWRFQVIPRTKS
                     DVAGNPASSPTVTVVSTPVISTVTPATGTVLGGDRVVIHGSQLKGVIQVTFGGIPAAQ
                     VIARADGALEVTTPPHSAGLVTVVVETVTGAVSKVNAFKYVDEPKVPDPEKRRNIFGC
                     GVTEDGSGSSLTADLLAALALLLMLGLKSRRAEQQPQR"
     gene            9652..9727
                     /locus_tag="PKN92_09970"
     tRNA            9652..9727
                     /locus_tag="PKN92_09970"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9685..9687,aa:Lys,seq:ttt)
BASE COUNT         2290 a         2585 c         2808 g         2445 t
ORIGIN      
        1 gcacacaagc gggtcatgat gaagacaagc cacccggatt cgccgcggaa atatgccccg
       61 gaacgtatct ccaactcaca gaccacaaaa gccccttgcc aataggtcga ttggcgttcc
      121 caggctggcg gattttatgt tttgagtcag agtattttgg tgtgggatcc atataagtct
      181 caagtagaaa ttcgtgtcat tcgtggttat gtccgtgcca ataggttaat tactgctctc
      241 agaagaggcg tcgacggctt aatatgactg gggtcatgcc agtccactct gtccactctg
      301 tccactctgt ccactgtctt tgatgtggat tgcactccta taaatcaaag gcgcccatac
      361 ccgaattaag attcctgcgt gcgccgtcat ttctcccggc cgcccgatat tcattcgtat
      421 ttatctccag agttactgaa agcctttgta agattttaga tggcctgatt cgacaagttg
      481 caccgctgca cggtcattgc gtactatcaa agacgtatga ttctttattt ccctgtgcct
      541 gaaacagaca ggtatttttg cagcgcagcc atcacccgac cgataaggag tgcagccatg
      601 ttttactctg gaatttccga cgaagccgga caggcaattg ccgtacagat taaagcccat
      661 caggaactgg gctggaacta tatggaattg cggcttgttg acggcgtgaa tctgacccag
      721 atatccgatg ctgcttttga cgatgtttat aaagcagtga ccgaggcgga catgaaagtc
      781 tcctgtttcg gcagcgctat tgccaactgg gcacgcccca ttacctgccc ggctgaaata
      841 gatagagacg atttgaagcg ggcaatccca agaatgcacc gtttccatac gccctttatc
      901 cgggtaatga gttatcccaa tgatgcggag catcctgtgg aggaaacggc gtggcgccgc
      961 gaagcaatta aacgcatgaa agaactggcg cgaatcgccg aggacaatgg cattacgctg
     1021 gttcatgaaa actgcagcgg ctggggcggt ttgtctgcgg aaaacagcaa tattctgctg
     1081 ggtgaagtgg acagccccgc tctgaaggtg gtgtttgata cgggaaatcc cgtgacctac
     1141 ggccaggatg cctgggagta ttacaaaaca gtacggaaag acattgtgta tgtgcatatt
     1201 aaagatgcac ggaaaataga cggtgtcgat cattactgcc tttgcggtga cggtgacggt
     1261 tatgtgcggg aaattgtggg ggatcttctg aaaagcggat atgacggcgg tctttccatc
     1321 gagccccacc tggcagcggt gattcatacc ggacagaagg cggacaacga cgagcagctt
     1381 tatcagtcct atacagacta tggcaggcgg ctcatggaaa ttgtagcgga agtgcgtgcc
     1441 tgatccggca aaaaggaaac agaaagaaat gaagcgaatt tctgtgatga cctctggcgg
     1501 tgatgcgccg ggcatgaatg ccgctatccg tgctgttgtc cgtacagccg tcgcaaaaga
     1561 ggtggaaatt tacggcgttg aacgggggtt tcaggggctg atcgaaggga tgttgcgccc
     1621 tctgaatgcc cgctccgtga gcggaatcat ccatcatggc ggtactattc tgaggacagc
     1681 gcgttctgcc gccttccgcg aagaggaagg cctgagtcgg gctgtgcgct cattggagaa
     1741 aaatggaatc gaagggcttg ttgttatcgg gggtgatggc tcctaccggg gagcccatgc
     1801 gctatggaag cattgcggta tcccctgtgt cggtattccc ggaaccatcg ataacgacat
     1861 cggcggcacg cagtatacca tcggatttga tacggcattg aatgttgcca tggaggcaat
     1921 agaccggctc cgcgacacgg cggcatctca tgaccgtact tttttcgtgg aagtcatggg
     1981 cagacacaat ggctttatag gcatgtgcag cggtcttgca ggcggtgccg aggaggtgtt
     2041 ggtacccgaa cagccctttg atctgccgat gctggtggag acactgcgtg caggcgtggc
     2101 acggggtaaa acctccatga ttgtgattgt tgctgaaggc ggtgatggcg gcggcgccta
     2161 cgaggtttca caaaaggtgg cggaagcctc cgaattcaag gacagccgca tcgcggtgat
     2221 cgggcatatg cagcggggcg ggacgcctac cgctttcgac cgcattctcg cctcacgcat
     2281 gggagtctcc gccgtagaaa acctgctggc aggcgcatca ggaatgatga ttgccgcagt
     2341 caacaacgcc atggtgcccc gtccctttga ggatgcctgg aatacgcgta atcttttcga
     2401 tccggaaatg ttacaggtat tgcatacact gggcgtataa tctcctgcac aagtgtcgct
     2461 gaagttaatg tctttaccaa caggatccgc cggtgaaact gtctgacaga tatattctcg
     2521 ggaaaatatg gccgcctgca ctgctcgccg cattcattat cagttttgtg gtcgtgggcg
     2581 gttccgtgcg gtcggaaatg cgcgccgtga cggacagtgt gccgatcagc cagatggcgg
     2641 tgctggatat tctgcgcctc ggtctgtatg tgctgcctac gctgtggggc tacattttcc
     2701 ccatcaccta ccttatgggg gttatgtatg tcttcagcag acttgcccag agcaatgaac
     2761 tgaccgcact gaaagcggcg ggaatttcac tcaggcgtgt cgtgatgcct gtcgttgtgc
     2821 tcggactgct gctgagtgtg gcggcttttt tcattgccga ccgcatgcag ccctgggcat
     2881 acgaacggct tgtccggctt atcagtcagg aactgcccat gcgtatgaca ctggaatcac
     2941 tgcccgccgg ggtcatgcac gaagtggggg actggcgtgt ttatattgcc gagcgggaag
     3001 acaatgggga tctgcggggc attgtaattt tacaaccgaa tgatgacggc gccaacgcct
     3061 tttatgcaga cagtgcccgg ctggtcatga aaaaagggag tctgcatctg gaattgaggg
     3121 acggctactt tatcccaagt gatccgcgcc agcattttat gttcaagtct cttgttaaag
     3181 aagcgcccat gccgaaaatg ctcaagcgcg tcctgtccgg agaaggaatg acactttctt
     3241 cgctcctgag cgaggaaaaa cggcttgccc ggcgctttga cgaaaccggg gcactgcccg
     3301 tgggtgtgga gctgcgcaat gtgcggcagg aagtgaaaaa caggcttgct tttcccctga
     3361 tgtgtctggc agtcagtttt gtgggtgccc ccatcggtgc acgctcgaaa aaagccggac
     3421 aatcctatgc cttttccatc gggctcagca tcataggtgc atatttcatt ttgcgaaaac
     3481 tggtggaact cgaagcgttg ctgcctctgg cgctgaccgt gtcgctggga cagatgccca
     3541 atctgctgct ctgccttctg ggcatcatcc tgctctggag ggttgaccgc gtatgaatgt
     3601 gctggatcgc tatcttgcag ggcgcctgct ttccgttttt gcgaaagtcg tgctgtctct
     3661 ggtgctgctg gtttgcctga ttgatctggt ggtcacacgc cagagcgcca ttgccagata
     3721 ccaggtgccc tggcttgtcg tccttcaata ttatctgact tttgctccca ccattctttt
     3781 tgaatatcat gcggctgctg tttccctgct ggtggcggca ctgattgtac tggggcgtgc
     3841 cgcacaggat aatgagattc ttgcgctcct ggctggtggc gtcagtctgc gaaggattgt
     3901 gagagcgccg gttgccgtgg cgttcttgat tgccttgctg gtctttgcgg tacaggaaag
     3961 ctacggggtt catgcctcaa aactgcacag gcatattcag agccgctttt tctccaagat
     4021 aaccgacagg acacggcacg gagttagctg gacaaatctc ggggacggtt ggacctgtca
     4081 tatcctgaaa ttcaacaccc gtgccaacag cggacaggat gtatacctgc atgctttcag
     4141 aggcaaacgg ctggaagaaa tccgggcaaa ccggatttat tgggatgaag agaaaagcaa
     4201 atggctgctt gaagacggac ggcgcgccat ctttgaccag gataaagaat gggaaattct
     4261 ttcttcaagg atcacccagg aggaagcccc gtttcaggag cctccggaaa aacttttcgc
     4321 tttatctgta tccgcccagg aaaaaagtgc aggggacctt tcaagagata ttgaagaagc
     4381 cagaagtatg ggcgtgcccg tacgggaaca tctggtcaac tatcatgtaa agtttgcaca
     4441 gcccgctctc tgctttgtca ttatatggct tgccataccc tttgcaatcc ggttgcgcaa
     4501 agggggaatt atgatgagtt ttggggtcag cgccatgatc gccctcgttt atctcgtgct
     4561 ttttgcagta ggggtggggc tgggttacat gggaacgctg cccccgcctg ctgcagcctg
     4621 ggcggccaat gcgctctttt ttattgtggg cctgctcctt ttccggcgtg tgcctacttg
     4681 atcacgggaa atgaaccgtt ttcctgtaga tcttcacttt gcttaatctg agaatgtgca
     4741 ggtaattttg taaagagttt acttgacaag tgattgtatt cgtgatagaa tgcgcaatat
     4801 aagagttgtg tcttatccta accgtaccct aaacggaggt gggttcaatg gccgatgata
     4861 aacagtttga caatgaaaac cccgaatttg aacacgaaat gaatgcaggc aaatttggtg
     4921 acgatgaggg tctggggaat ctgccgccac tgagcgatct ggattctcag ggcacctacg
     4981 actccgattt ggacctgccg ccactgggag gttttgaatc gcaggatact tccgtgggag
     5041 gcggagcgcc ggatcttctg ggacagacgc ccagcggaac gcccttcaca agcgacttcg
     5101 atgcaagtgc cttcaatttg gctggagatt ccggcccggg cggtcatccc tccggaccgc
     5161 tcatcggagg atttcaggat ctcgcagcgg acagcgattt ccacccggaa acacctgaca
     5221 tcggttccgg tccggatgcg acactggaca cgcccatttg ggatgacagc gctttcggcg
     5281 gatcgggtgg cgatctcttt acgcccggtg ctagcgagaa cgtaacacag gcaacgcctg
     5341 cgcccgcttt tgatgatttt ggtgatgcgg gaagctttgg agatttcggc ggaacgccta
     5401 tgccgggcat ggcgggcggt acagggatgg acgattttgg cgcagttcct gtgggagccg
     5461 gtgctccgtc cgtttccggt gctcagccca aagcacggca gggtgccggt gcgctgctgg
     5521 taattttcat cggtctcgta tcgctcggcg tcggcatgat tgcgtcacag cccctttcca
     5581 catacctgac ctttgtgccc ggtaatccgc tcgtaatgga aatgcagacc aaagatgcca
     5641 ccatcgccaa cctgaaccgg cagatcaata cgttgcagca gcttcaggcg accccggatc
     5701 ggcctgccga catcagcccc gaaagagtcg ctgaactgca gaacgaaatt ctggtgcgaa
     5761 cacaggaact ggacgccgtc aaaggacaac tcgattccac ccgcgccacg ctgtccaccg
     5821 aacaggcaga actcaccagc gttgaaagag aattgcgtga acgcaatgac gaagtgattg
     5881 ctgcaagaga atttctcgaa gaactgcgca gtgaaacagc gatcgtgcag gcgcgtcagc
     5941 gcggactcat cgccgaagtg gatcggctca ccggctatgt cggcgaactc gatgaagcca
     6001 atgcaagacg tatcgccacc aaagaagcgc tggagcacaa tatcgaccgc ctcgttatcc
     6061 aggtgaaaga aagcattccg ctgacccccg aaaaatacga ctatgccaca cgcctggctc
     6121 aggtggaagc gctgcgggac aaagccgcac aggccggatg ggtctcccct gaactgcaga
     6181 atgaatatac cagcctttat ctcaaagaac ttgagctcgc acgcgccaat gattatttct
     6241 ttgcctgtgt gcccgttacc gaccgcttcg gacacacttc catgtgctgg gcagaatgcc
     6301 tgatgcgtgg gaattgggcg gtctattatc gctctctgga cggaaaaggg atcggcagtt
     6361 tcgagaatgc aggctcaacg gaaacacccc gctgggaatt caagcagaat ctgtccgaag
     6421 atacacggct cgccatagaa gcgcatattg tcagtgcacg gattcctgat ttcgaagggc
     6481 atcttcagcg cctggcagaa cgtgaactga aggctcagaa agacactccc tggcagcgtg
     6541 tctacagctc catgtgatat gtaaggtgcc ccgggcagcc ggatgtgttt cccgaaagtg
     6601 cctgtgcagg ttgtgcgcag gcaggcgcat accaaccggc agcttctcgg ggcttcgtat
     6661 aaaaacgggc cataggcttg tgacgtgaac gggagagaat catgatgcct gagaccaagg
     6721 cgttttgcgt aaacaaggca aaagcgacag catacctggt agtatgcctg ataatggcct
     6781 tcacggtttt tcaggcaaac gccgacgaca ccgccttgct catggataaa cagttttttg
     6841 atctgggatt acaggcgatc ccctcatccg atggatccga ctgcgccatc caactgatct
     6901 ggatagattt ggagcagttg ctgcaggaaa acggatttcc gcctcaaccg ctttttgtct
     6961 accgctctga aattgcctat cccggagacg atggaagtgg cggagacgta gtctatgcgg
     7021 gctctaccgc acagaagggg ctggtggaca gcgatgtggc cgtcggtgtc gagtactact
     7081 acactttggc ggttggcacc tacggcaccc agtattttct ttctgatacc gcacgcggtt
     7141 cctgtacagg aagcaatctg ggactgcggg cttctgcgct gcacgatatt gatggcaaat
     7201 gctatattgc tttgacctgg cagtcggtcg gtgagcctgt ctctttgtac cgttccacgc
     7261 tgggctatag cagcccgccg gacagccaac tcgtctttgg cggttccaaa gaagacaata
     7321 tctggctgga tgaaaacgta accgatggac tccagtattt ttataccctc gtagccggtc
     7381 aagatattat tgaccccaag gctgtttctt ttgcctccgc tgtcgcatgc gatgatccca
     7441 tgccggttta taccgaggtt tttaataaca caaatccctt cgatcttgcc tattcacaga
     7501 ttattttccg gcccgtaaaa gaagatccgc accacctgac agatgacctg tccgatatgt
     7561 cttatgcgga ctacgaagtg acagtgctta ataatgtgac cgagctgccc gttcccagat
     7621 ccgatcattc aggtttcgga agagatctgg ctcttggcga aaacatgcag tttcccgttc
     7681 agataggcgg agaaaaagca gcctctgttt tcttcttcgg aaaacggtac agcacactca
     7741 cggtaagcgc aaacggatat atctcctttc aggagcaatt cggtaccgcg cctttcaaag
     7801 aaacgacgat ggtggaagag cattttagtg agccgcgcat cgcctttttt aattcagcac
     7861 tgatgccgca gctgggcgga acagtctggg tgcgtctctt tgatgacagc atcgtgttca
     7921 ctttccagaa tgttacaata aaagcaccga cgggcatgct caaccccgac aggattaccg
     7981 cacaggtcga agtcttcttc agcggacata ttcgcatgac ctatctcgaa gcaacgatgc
     8041 ataccggact tatcggcatt tcggatggcc ggggggtacc cgctgaaccg tcaggcgcct
     8101 atccggctct cgggttgagc ggtgcccata actgggtgcc tttcgactcc ctgccggaag
     8161 tgccgcaacg catcagcctt ctgcccatag cctggccggc ggtgacagca ggggagcgca
     8221 tcagttttat agccgatatg gaactgcccc ccggtacggg cagcgttccg gcattttacg
     8281 ggctgtggtc tggtgaaggt gcggctcctt ttacggacag gggcgacggt accggtcttt
     8341 tcgactggac gccagccata acgctttccg gcttctataa agtgcgggtt gtagcgcttc
     8401 tcggtgcaga gcgggtgttt caggatgtga ttctgacggt gagatctgtt gaccccaggg
     8461 cattgctgcc gcgcgctctt aaacttcagc tgagtagccg tgaaggagac gctgatccgg
     8521 aagacgatca tgcggtgtat gccggagaac cgctttttgc ctcctacact tatgaacatc
     8581 ctctggcgaa agaaaatcct gccctttatg gcgaaggcgc aacgcgtatt tactggtata
     8641 aaaacggaca gattgtaact gcctacactg atcgcagctc cgtgccttcc ggcgtcaccc
     8701 gtttcggtga tgagtggcgt tttcaggtga tcccacgaac caaaagcgat gtcgccggaa
     8761 atccggcttc ctcgcctaca gtaactgttg tcagcacccc agtcatcagc accgtgacac
     8821 cggcaacggg tacagtgctc ggtggagatc gtgtagtaat ccatggatct cagttgaagg
     8881 gagttatcca ggtcaccttt ggcggtatcc cggctgctca ggtgatcgca cgggcagatg
     8941 gtgcactgga agtgaccaca ccgccccatt ccgccgggct ggttaccgta gtggtcgaaa
     9001 ccgttaccgg tgcggtaagt aaagtgaatg cattcaaata cgtggatgag ccgaaggttc
     9061 cggatcccga gaagcgcaga aacatcttcg ggtgcggtgt gacagaggat ggaagcggct
     9121 catcgttgac agccgatctc ctggctgcgc tggcattgct gctgatgctt gggttgaaaa
     9181 gccggagagc tgagcagcag ccgcagcgct gattcttaac atgagtgcta caccgcagca
     9241 attctctgat ggattgctgc ggttttttat tggcgcatac tttcagcccg gaaccgcgtt
     9301 ccctgcattt ctttttcttt tactcctcat tgaagaggaa agccctcccg ataagcctgt
     9361 cattcttttc aggaaaaccc ggtatgcaag cggcttgtta atttcgtcct ggcttttttc
     9421 gaaacttcac gcgggggcat tgtgtgctct tggagcttta catccaaaag cgtattggaa
     9481 agaagatatc cctaaggcct tttttgtcac ccataattgt catttaaaaa tgagctcatt
     9541 ttgagccgcc ttatacgcaa aagttgattt gcccccaaca gttcctatat aatgcgactc
     9601 tcatcaaaaa tacccagtac ccgaaagggt acctctgttt atcaacatag tggacgggta
     9661 gctcagccgg tagagcatcg gacttttaat ccggtggccg cgggttcgaa tcccgccccg
     9721 ttcaccattc ttaaatcttc aaaacccctg caatcattgg ctgaaacgct tatggatgca
     9781 gggtttttct tttgatccga tcattgaagc ataagtagaa atattggcac ttgcagaccg
     9841 aaaatgacat gtcggaccgc ctggaacaga tgcgtttgcc caccattttc ttcagtgttt
     9901 acatccggga gcgcatcctc ccggatttaa aaagtctgtt ttaggcatat cttttttctg
     9961 aacctgcaaa acagatggcg gtgcgctccc gtccttgacc gctgtttatt acgctgttgt
    10021 cccgtcttca ttgctcgctg ccggaatgcg ataagccgcg tccccacaga tcaggcgcgg
    10081 gttcaaattc gaaacgcaat tattgagaaa caaaaaagga gacattgc
//
LOCUS       DAOPWB010000113         9961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6830_length_9961_cov_5.269231, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000113 DAOPWB010000000
VERSION     DAOPWB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6830_length_9961_cov_5.269231"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..579)
                     /locus_tag="PKN92_09975"
     CDS             complement(190..579)
                     /locus_tag="PKN92_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNZ49162.1"
                     /translation="MKETTVPVPTEPNVPDTREETRTLKPPVDIFETDTGLVVVADLP
                     GVDKDSIEVDVEKNVLTLKARPSQELKEDFLRQEYELLPFFRQFQLSERVDQAGITAE
                     MKYGVLTIHLPKVKEQQPRKIDVTVTA"
     gene            complement(619..1083)
                     /locus_tag="PKN92_09980"
     CDS             complement(619..1083)
                     /locus_tag="PKN92_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNZ49163.1"
                     /translation="MTHIFWEPFQQLQAIRNEMERVFEQYTPSSRAYARSAFLPSLGA
                     RRYPLVNIREDQDKIYVEALAPGLNPGSIEVSVLRDQLRLSGEKAAINPDVKAEAWHR
                     NERGAGRFVREISLPAEVDGDKVTAAYKNGLLLLTLPKHEAAKPRQISVQVS"
     gene            1484..5509
                     /locus_tag="PKN92_09985"
     CDS             1484..5509
                     /locus_tag="PKN92_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ49164.1"
                     /translation="MQFCLCPRRNLIPFLFVLILSAFFSASGLYAQEEGWSEALSAAR
                     DQFQSRDEASASEAVTLSAWYKTAVFANVAFDDVALPEQSRHLNERNEDGGRLWLRDR
                     DLQDGIVNSLTCGDGSALYLARTIRIQEPILLNIGLGSDDGIKVWLNGALVHSNNAAR
                     VLSPEADTLTLPLLDKDNFLVVKIYNVSGGCGFYFNIRDDGAKNLWRGVEKSYPQACL
                     RMAQDLGGTDPSEWIASADPTELSAAMVSKRLQGMKEIQNRWQEKWDAMTASGAGALT
                     QLQFYQHLCESGALLERFNQINREALRLAVEYLVKEFPDRYAGGRAYLDELAALEAQL
                     PEYFAALGENAPEARDITARWEALQRTALLANPLLDFEKILLVKRHERQLGLPQNWQG
                     NCSMPRRGYDNELALLDYRTQNSQPETLFRPEKDYFVGDVDLHFDGDRMLFSMIGSHN
                     RWQIWELNSDGTGLRQVTPGAEEDVDNYDACYLPDGRIIYDSTLCMQGIPCVTGSDAV
                     ANLCIMNADGSGIRQLCFDQDHDWCPTVLNNGRILFSRWEYSDTPHYFTRVLFHMNPD
                     GTNQVEYYGSNSFWPNSMFYTRPIPNNPSQVVTIVSGHHGVARMGELVLLDSARGRHE
                     ASGVVQRIPGRGQPVEPVIEDQLVDEIWPKFLHPYPLSEHFFLVSCKPSPQQPWGVYL
                     VDTFDNMLLLHEEKGYALFEPLPFRAIATPPAIPDRVNLEAKDASVYLMDVYAGEGLK
                     DVPPGTVKALRVYSFHYCYQHMGGHEHVGVEGPWDVRRILGTVPVMPDGSAFFKVPAN
                     TPIAVQPLDSEGKALQVMRSWFTAMPGEVLSCVGCHEPQNTTPSLHYNRAARRTPDQI
                     SPWYGLARGFSFHHEIQPVLDRHCAGCHDRDDYPDGRPNLSNRSGREHANFTLSYLAL
                     HPFVRRPGPESDYHLQIPLEWHADTSELIQMLKKGHHGVQLDTEAWDRLTTWIDLNVP
                     DHGRWTDHTDKAKACAGRRAELRALYACTPEDNSEADLVEAAYPREFVKHEKPALDAP
                     APALESWPFDRAQAEARQAQAGSIQRRTVDLGNGTTMEFALIPAGKFVMGGDRFADEY
                     PRTVVEIEKPFWLGTTEVTNEQYRCFNPAHDSRYIDQHSKDHTTPGYPANEPLQPVIR
                     VSWNDALAFTQWLSEKLQVPCTLPTEAQWEWACRAGTDSPFYYGGPDTDFSAFANLAD
                     LSTRKLAVAGVNPQPIANPNRFQDFFPKDARFDDGQRIVCVVGMYEPNVWGLFDMHGN
                     VSEWTLSAWRPYPYQEQDGRNNPDVQEKRVVRGGSWSDRPKRARSEFRLAYEPWQGVF
                     NVGFRVALPCTEEESMLAAAH"
     gene            complement(5557..5922)
                     /locus_tag="PKN92_09990"
     CDS             complement(5557..5922)
                     /locus_tag="PKN92_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49165.1"
                     /translation="MNLFWGARLAQDLRDVQNQIEKDAQVARQQRMITTETNDLRNEV
                     ERLCLLNQAMWELICSKLGLTDADLEKKAQEVDLRDGKLDGKMTTHPVRCPQCGRVSN
                     SRLKKCMYCNMEFEGSAFG"
     gene            complement(5919..6728)
                     /locus_tag="PKN92_09995"
     CDS             complement(5919..6728)
                     /locus_tag="PKN92_09995"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ49166.1"
                     /translation="MKKRFQDKVALITGASSGIGEALARQLAAEGAGLCLFARRADRL
                     QALQSELEASGAQVAVYAGDVTVPSDLEGAVALALERFGRLDLVVANAGIGVTSPFSR
                     LTTEDYRHQFEVNFFGVLHTLFASLEALKASQGQAVLVGSVAGLAGTPGSSPYNASKF
                     AVVGLAESLYYDLGKEGIAVTLVNPGFVESEIRLLDNRGARTGRGDPVPSWLVMPASK
                     AASVILKAVYKKKAVVVVTFHGVVLSWLKRFCPALIRLLLRQGSYRKERKK"
     gene            complement(6855..8222)
                     /locus_tag="PKN92_10000"
     CDS             complement(6855..8222)
                     /locus_tag="PKN92_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ49167.1"
                     /translation="MSKHTLTRRAFLSTATTTALVAGYASGNPLVNTARVIPRKLSPN
                     ERLNIASIGVGGMGHGDVMKCRSENIVALCDVDFKRAEETFYRLPKAKQYKDFRVMLE
                     EMDKEIDAVTVSTPDHIHAPAAYLAMTMGKHVRVQKPMTQTIAEARLLAKTAKETGVV
                     TAMGNQGHSGNGIREMCEIIWSGAIGQIRESHTWTDRPIWPQGIANRPAAQPVPEHLD
                     WDLWLGPAPWREYSAEYLPFNWRGWLDFGCGAIGDMACHIVDPVFWALKLYEAPSYTV
                     EVVQQTGLTQETFPNSSIIKYEFPARGDMDPVTVYWYDGGLLPKRPEGVPENEKLGDG
                     ENGSLFIGTDGVLTTGTYGGDTRMLPAERMKDFTMPTPTLERIPDENPYMHWINACKN
                     GTEAVSPFSYAGPLTEFANMGNVALLAGAKIEFDVASMTITNNAAANQFLTKSYRKGF
                     DFMPL"
     gene            8439..9446
                     /locus_tag="PKN92_10005"
     CDS             8439..9446
                     /locus_tag="PKN92_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNZ49168.1"
                     /translation="MRIGSFDIEHPLCLAPMEDVTDLPFRKLCSDFGAQVLFTEFANC
                     EAVIRNIDSEISKFNIEKDARPIGIQLYGSNPESLEKAAHIASASYPDFIDLNCGCWV
                     KKIAGRGDGAGLLRDLKRLEAAVQAVQRGSSVPVSVKTRLGWDNEQYVILELAPRLRD
                     MGIQFLTVHCRTRVQGYRGDADWSWFPRIKEAAPDLPLIGNGDITTPKEAAACFSLGC
                     DGVMIGRAAIQQPWVFRAMRSYLEDGVQLPEPTLDEQLDWCIAHLRAHVAFRGVPRGI
                     YSFRRYYAAYLRGVTNIARLRRDLMQLMDTEAIVDRIRAFQSNRAEEDTASAEEDAPA
                     L"
     gene            complement(9618..>9961)
                     /locus_tag="PKN92_10010"
     CDS             complement(9618..>9961)
                     /locus_tag="PKN92_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49169.1"
                     /translation="IAIHIRQTVVSITNLGNGNYRLSDVNRDTVVTAVFVKKMRSIAY
                     KADGEGTVTGLHSVQDTTGAVEFTVEPAPGKSFAGIDYDNTKALVEHVGGTTYRVRNV
                     TANIQITAYFM"
BASE COUNT         2189 a         2839 c         2633 g         2300 t
ORIGIN      
        1 gcaaatggcg tgacgaaagc cgctacggta agattagcaa atggcttgac aggatccttg
       61 tcaatgccta tcccggagca tagtgccata aaaaagaagc cggacggacc gcagatcttc
      121 tgtttgggtc cgcccggcga caacatcgga cgggagttcc gatggttcat gcctcggttg
      181 attttgtgat tatgcagtca cagtcacgtc aattttccgg ggctgctgtt ctttcacttt
      241 cggcaggtgg atggtaagca cgccatattt catttcggcg gtaatgcctg cctgatcaac
      301 acgctcgctc agctgaaact gcctgaagaa aggcagcagt tcatattcct gacggaggaa
      361 atcctctttc agctcctgag aggggcgcgc cttcaaagtc agtacatttt tctccacatc
      421 cacttcgata ctgtctttat ctacgcccgg cagatcggca acaaccacca gcccggtatc
      481 tgtctcaaaa atgtccacgg gcggtttcag cgtgcgtgtt tcttcacggg tatcaggcac
      541 gttcggttcg gtcggtacgg gtacggttgt ttctttcatg atatctgtct cctttttctg
      601 tttttctttc tgtggttctc agctcacctg aacggaaatc tgcctgggct tcgccgcttc
      661 atgtttaggc agggtcagca gaagcaatcc gttcttatac gccgcggtca ctttgtcgcc
      721 gtccacttct gctggaaggc tgatctcccg gacgaatcgc ccggcaccgc gttcgttgcg
      781 atgccatgcc tctgctttca catccgggtt aatggcggct ttctcaccgg agagtcgcag
      841 ctgatcacgc agtacggaga cctcaatact gcccgggttc agacccggcg ccagcgcttc
      901 cacatagatc ttatcctggt cttcccggat attcaccaac gggtagcggc gtgcaccgag
      961 ggagggcaga aaagcggacc gggcataagc gcggcttgaa ggggtgtact gctcaaagac
     1021 acgttccatc tcgttgcgga tcgcttgtaa ctgttgaaaa ggttcccaaa aaatgtgtgt
     1081 catggctgaa agatcctttc tatttgtttt tccttgtcag ccggccgccc cggcggagcg
     1141 gccggccttt ctttgcatgc ccaactgtat atggcaagtt gcgtgccaaa acagaaaaag
     1201 agcattataa aaaaacatat cttactgaat atgaacaaga tattgatttt tctttggaga
     1261 ctgctgacgc gcctgtgttt ttggaaggcc gtctctgtct gattcaaaag gaaacagtac
     1321 tccttccttt tgaaacagca ccgcagtacc ctttgtcgtc gagcccctcc gccgtattcg
     1381 ctctttcgcc cgcatctctt ttttgtttgg ctcttttcag cgtccttgct atacttattt
     1441 atgtgcactg ttaacaaacc tcgtccagaa gaaagagagt tccatgcaat tctgcctttg
     1501 cccccgccga aatctgattc cgtttctttt tgtgctgatc ctttccgctt tcttttccgc
     1561 atcaggactt tacgctcagg aagaaggatg gtcggaagcg ctgtcggctg caagagatca
     1621 attccagagc cgtgatgagg catccgcctc ggaagcagtc accctaagcg cctggtacaa
     1681 aacggcagtc tttgcgaatg tcgcttttga tgatgtggcg cttccggaac aatcccggca
     1741 tctcaatgaa cgcaacgagg acgggggccg cctgtggctc cgtgatagag accttcagga
     1801 cggcatcgtc aattctctta cctgcggcga tggttcggca ctatatctgg cacgcacgat
     1861 tcggattcag gagccgattc tgttaaacat cggtttggga agtgacgacg gtatcaaagt
     1921 ctggctcaac ggagcacttg tccacagcaa taatgcggca cgggtactgt ctcccgaagc
     1981 ggacaccctt acgctgcccc tgcttgataa agacaatttt ctggttgtta aaatttacaa
     2041 cgtcagcggc ggctgcggct tttatttcaa tatccgggat gacggcgcca aaaatctctg
     2101 gaggggtgtg gagaaaagct atcctcaggc gtgtctccgg atggcacagg atctgggcgg
     2161 aaccgatcca tctgaatgga ttgccagtgc tgatccgacg gagctttcag cggcaatggt
     2221 atcgaagcgc ctccagggca tgaaagaaat acagaacagg tggcaggaaa aatgggacgc
     2281 tatgacagca tccggtgcgg gggcgctgac acagctgcaa ttttatcagc acctgtgcga
     2341 gagcggtgcg cttctggagc gtttcaatca gatcaaccgg gaggccttaa ggcttgccgt
     2401 cgaatatctg gtcaaggaat ttcccgaccg ttatgccgga gggcgcgcct atctggacga
     2461 actcgccgct cttgaagcgc agctgcctga gtattttgcc gcactggggg aaaatgcccc
     2521 cgaagccagg gatatcaccg cccgatggga agcgctgcag cggacagcgc tgcttgccaa
     2581 tccactgctt gactttgaaa aaattcttct ggtgaaaagg catgaacgcc agctgggcct
     2641 gcctcagaac tggcagggga actgctccat gcccaggcgc ggttacgaca atgagctggc
     2701 tctattggac taccggactc agaacagtca gccggaaacg ctgttccgcc cggaaaaaga
     2761 ttattttgtg ggcgatgtcg atctccactt tgacggcgac agaatgctct tctccatgat
     2821 cggcagccac aaccgctggc agatatggga attgaacagc gacggcaccg gtctgaggca
     2881 ggtaacgccc ggcgctgaag aggatgtgga caactatgat gcctgctatc ttcctgacgg
     2941 gaggattatc tacgactcca ccctctgtat gcagggcatt ccctgtgtga ccggaagcga
     3001 tgctgtcgca aacctctgta tcatgaatgc cgatggaagc ggcatacggc agctctgctt
     3061 tgaccaggat cacgactggt gtcccacagt gctgaacaac ggcagaatcc ttttcagccg
     3121 ctgggaatat tccgatacgc cgcactattt tacgcgcgtc cttttccata tgaaccccga
     3181 cggcaccaat caggtggaat actacggcag caattccttc tggcccaact ccatgttcta
     3241 cacgcggccc atcccgaaca accccagcca ggtggtcacc attgtgtcgg gtcatcacgg
     3301 cgtggcacgc atgggcgaac tggtacttct tgattcagcg cggggccgtc acgaagcaag
     3361 cggcgtggtt cagcggattc cgggcagagg acaaccggtg gagcctgtca tcgaagacca
     3421 gcttgtggac gagatctggc cgaaatttct gcatccctac ccgctcagtg aacacttctt
     3481 ccttgtttca tgtaaaccat ccccgcagca gccctggggt gtttatctcg tcgacacttt
     3541 cgataacatg ctgcttcttc acgaagaaaa gggctatgcc cttttcgagc ccctgccgtt
     3601 ccgtgcgata gcaacgccgc ccgccatccc cgatcgggtt aaccttgaag cgaaggatgc
     3661 gtctgtctat ctgatggatg tctatgcggg cgaagggctg aaagatgtgc cgcccggaac
     3721 agtaaaagcg ctccgtgttt attctttcca ttactgctat cagcatatgg gcggtcatga
     3781 gcatgtgggc gtggaagggc cctgggacgt gcgccgtatt ctgggtacag tccctgtgat
     3841 gcccgacggc tccgctttct tcaaagtgcc cgccaacaca cccattgccg tacagccgct
     3901 ggacagcgag ggcaaagccc tgcaggtcat gcgcagctgg tttaccgcca tgcccggcga
     3961 agtgctttcc tgtgtgggct gccacgaacc gcaaaatacc acaccttccc tgcattacaa
     4021 ccgggcggcg cgccgtacac ccgaccaaat ctcaccctgg tacggacttg cccggggatt
     4081 cagcttccat cacgaaatcc agcccgttct ggatcggcac tgcgcaggct gccatgaccg
     4141 tgacgattac cccgacggca ggcccaacct cagcaaccgg agcggcagag agcacgccaa
     4201 tttcaccctg tcctatctgg cgctgcaccc cttcgtccgc cgacccggtc cggaaagcga
     4261 ctatcatctc cagatccccc tggagtggca cgccgacacc agcgagctga ttcagatgct
     4321 taaaaaagga catcacggcg ttcagctgga tactgaagcc tgggaccgcc tcaccacctg
     4381 gatagatctg aatgtgcccg atcatggcag atggaccgac catacggaca aggctaaggc
     4441 ctgtgccggg cgccgtgccg aattacgcgc tctttacgcc tgcacgcccg aagataattc
     4501 ggaagcggat ctggtggaag cggcatatcc ccgggaattt gtcaagcatg aaaaacccgc
     4561 tctggatgca ccggcaccgg cactggaaag ctggcccttt gaccgggcac aggcagaagc
     4621 ccggcaagca caggcgggca gcatacagcg ccgaaccgtt gacctcggca acggcaccac
     4681 catggaattt gcactcatcc ctgccggaaa gtttgttatg ggcggcgacc gcttcgccga
     4741 tgaatatccc cggactgtcg tggaaattga aaaaccgttc tggctgggaa ccaccgaggt
     4801 gaccaatgaa cagtacaggt gtttcaaccc tgcccatgac agccgttaca ttgaccagca
     4861 ttccaaagac catacaaccc cgggttatcc ggcgaatgag ccgttgcagc ccgtcatcag
     4921 ggtcagctgg aacgatgcgc tggcgttcac gcagtggctg tcggaaaaac ttcaggtgcc
     4981 ctgcacgctg cctacggagg cacaatggga gtgggcgtgt cgcgccggta cggacagccc
     5041 cttctactac ggaggcccgg atactgattt cagcgccttc gccaatcttg cggatctctc
     5101 aaccagaaaa ctcgcggttg caggcgtgaa tcctcagcct atcgccaatc cgaaccgctt
     5161 tcaggacttc ttccccaagg atgcccgctt cgatgacgga cagcgaattg tctgcgtcgt
     5221 cggcatgtat gaacccaacg tctggggact cttcgacatg cacggcaatg tgagcgagtg
     5281 gacactctcc gcctggcgtc cttatcccta tcaggagcag gacggaagaa ataaccctga
     5341 tgttcaggag aaacgtgttg tgcgcggcgg ctcctggtca gaccggccga agcgtgcccg
     5401 ttccgagttc cgcctggctt acgagccctg gcagggcgtt ttcaatgtag gcttccgtgt
     5461 cgctcttccc tgcacggaag aggagagcat gctggcggct gcacattaag cataccggca
     5521 ggaaaccgct gtatcccctg aatacggggg cggtgctcaa ccgaaggcac tgccctcgaa
     5581 ttccatgttg cagtacatgc attttttaag gcgtgaattg gaaacccgcc cgcattgggg
     5641 gcatcgtacc ggatgcgtcg tcattttgcc gtctagcttg ccgtcccgga gatccacttc
     5701 ctgcgccttt ttctccaggt ccgcatcggt cagcccgagt tttgagcaga tcagttccca
     5761 cattgcctga ttcagcagac acaaacgctc cacttcgtta cgaaggtcgt tggtttccgt
     5821 ggttatcatc cgctgctgcc gggcaacctg cgcatctttc tctatctgat tctggacatc
     5881 tctgagatcc tgtgccaacc gggctcccca gaataaattc atttctttct ctcctttctg
     5941 taactgcctt ggcgaagcag caggcggatt aacgccggac aaaaacgctt cagccaggac
     6001 agcaccacgc cgtgaaatgt aaccaccacg actgctttct ttttatacac cgctttcaga
     6061 ataacagatg ctgcttttga agcgggcatg accagccagg agggaaccgg gtcacctctg
     6121 cctgtgcgtg cgccccggtt atccagaagc cggatctcgc tctccacaaa tcccgggttg
     6181 accagcgtca cagcgatgcc ctcttttccc agatcatagt aaagagattc ggcaagcccg
     6241 actacggcaa atttgctggc gttataaggc gagctgccgg gtgtaccggc aagccctgcc
     6301 acgctgccca ccagcacagc ctgcccctgc gatgccttca atgcctccag agaagcgaat
     6361 aaggtgtgga gcactccgaa aaaattcact tcaaactgat gccggtaatc ttccgtggtc
     6421 agcctcgaaa acggggaagt gactccgata cccgcattgg caaccaccag atccagcctg
     6481 ccgaaacgct ccagcgccaa agccacagcg ccttccaggt ctgacggaac agtgacatca
     6541 cctgcataga cagcaacctg cgcaccggat gcctccagct ccgactgaag cgcctgcagc
     6601 cggtcggcac ggcgggcaaa aagacagagc ccggcacctt ccgccgccag ctgacgggca
     6661 agggcttcgc ctatgccgga tgatgcgcct gtgattaacg cgactttatc ctgaaagcgt
     6721 tttttcatcc gtcttccttt tctgtaaccg cgctgaaaaa tgaggagccg ggactggaga
     6781 attgctcaca gaccccggct cctgacaata ctgatcaggc cggtcgctgg cactccgcct
     6841 gaacaaaaaa ggaatcacag aggcatgaag tcaaagcctt tgcgatagct cttcgtcaga
     6901 aactgatttg ccgccgcatt attagtaatg gtcatggacg ctacatcaaa ttcaattttc
     6961 gccccggcaa ggagagcgac attgcccata ttggcaaatt cggtcagcgg tccggcatag
     7021 ctgaagggag ataccgcttc tgttccgttt ttgcatgcat taatccagtg catatacgga
     7081 ttctcatcgg ggatgcgctc gagtgtcggt gtcggcatgg tgaagtcttt catgcgctcc
     7141 gcaggcagca tacgggtatc gccgccatag gttccggtgg tcaacacgcc gtccgtgccg
     7201 ataaagagtg aaccattttc gccgtcacca agcttctcgt tttcgggcac gccttccgga
     7261 cgcttgggca gcagaccgcc atcgtaccag tatactgtca ccggatccat atcgccccgg
     7321 gccggaaatt catatttgat aatggaagaa ttggggaaag tctcctgtgt gagcccggtc
     7381 tgctgcacca cttccaccgt ataagaaggt gcttcataca gtttcagtgc ccagaaaacg
     7441 gggtcaacaa tgtggcacgc catatcaccg atggcaccgc agccgaaatc cagccagccg
     7501 cgccagttga acggcagata ttcagcactg tattcgcgcc agggcgccgg accgagccac
     7561 agatcccagt caagatgctc gggcacaggc tgtgctgccg gccggtttgc aataccctgc
     7621 ggccagatgg gtcggtcagt ccaggtgtgg ctttcgcgta tctgaccgat ggcgcccgac
     7681 cagataatct cgcacatctc gcggataccg ttaccggaat gtccctgatt gcccatggca
     7741 gtcacaacac cggtttcttt ggcagttttc gcaagaagtc tggcttccgc aatagtctga
     7801 gtcatcggtt tctgaacccg tacatgcttg cccatggtca tcgccagata agcggcaggt
     7861 gcgtggatat gatcgggtgt agacacggtg accgcatcaa tttctttgtc catctcttcc
     7921 agcattaccc ggaaatcttt atactgtttt gctttgggaa ggcggtaaaa agtttcttcc
     7981 gcccttttga agtccacgtc gcaaagagca acaatgttct cgctgcggca tttcatcacg
     8041 tcaccatgcc ccatgccgcc cacaccgatg gaagcaatat tgagccgttc attgggggac
     8101 agtttgcgcg gaatgacccg tgccgtgttc acaagcggat taccggatgc gtatcctgcc
     8161 acaagggcgg tagtggttgc cgtcgacaga aaagcgcgac gtgtcagggt gtgttttgac
     8221 atgctgaaaa gcctccgtac aaagggttgg attttttata aaaacagcga cggagccagc
     8281 cagtccagcc cgaacgctgc ccgctttctg atgctgaatg aaccgccatc cggcatcatg
     8341 aaatatgatc ctctctttac ggactcattg tcaagcccgt tgactttttg gctgtctttc
     8401 cgtcctgagg gtatcatata ccataaaagg aagttaccat gcgcatcggc tcttttgaca
     8461 tagaacatcc tctctgtctt gctcccatgg aggatgtcac agatctgcct ttccgaaaac
     8521 tgtgcagtga tttcggtgca caggttcttt ttacggaatt cgccaactgc gaggccgtga
     8581 tccggaacat tgattctgaa atttccaaat tcaatattga aaaggatgca cgccccatcg
     8641 gcattcagct gtacggaagc aatcctgaat ctctggaaaa agcggcacac atcgcttccg
     8701 catcatatcc ggactttatt gacctgaact gcgggtgctg ggtgaaaaaa attgcaggcc
     8761 gcggtgacgg agccgggttg cttcgggatc tgaaacggct tgaagcggct gtgcaggctg
     8821 ttcagcgcgg atcctccgtg cccgtcagcg taaaaacacg cctcggctgg gataacgagc
     8881 agtatgtgat cctcgaactt gcgccccggc tgcgtgatat gggtatacaa ttcctcactg
     8941 tccactgccg cacccgggta cagggctata ggggtgacgc cgactggtct tggtttcccc
     9001 gtatcaaaga agcggctccc gatctgcctc tcatcggcaa cggcgatatc acaacgccca
     9061 aggaagccgc tgcctgtttt tcactgggct gcgacggggt tatgatcggt cgcgccgcca
     9121 ttcagcagcc ctgggtattc cgtgctatgc gttcctatct tgaagacggt gtccagctgc
     9181 ctgaaccgac cctcgatgag cagttggact ggtgtatagc ccacctgcgc gcacatgtcg
     9241 cttttcgggg cgtaccgcgg ggcatctatt cttttcgtcg ctattatgcc gcctacctca
     9301 gaggggtgac caatattgcc cggcttcgaa gagatctgat gcagcttatg gatacagagg
     9361 caattgttga ccgtatccga gcgtttcagt cgaatcgtgc agaagaggac actgcctctg
     9421 ctgaagagga tgcgcccgct ctctgaatcc acatcagcaa cgcgacttag catgtctctc
     9481 ccacgcaaca ctcctgaaaa aggctgcgac cgccgggctt ccaggtgtgc acggcggccg
     9541 catattcagg tatagggcaa gtgtgatgcc tgagggtgtc gttatcggtc tttcagatga
     9601 aattcctttc cagcacttta catgaagtaa gcagtaatct gaatgtttgc cgtcacattt
     9661 ctcactcgat aggtagtgcc gccgacatgc tcgactaatg ctttcgtatt gtcatagtca
     9721 attccggcaa agctttttcc cggtgcaggt tcaacggtaa actccaccgc tcctgtcgta
     9781 tcctgcacag aatgcagacc ggtgaccgtt ccttcaccat ccgccttgta ggcaatactg
     9841 cgcatctttt tgacaaaaac agccgtaacg acggtatcgc gattcacatc cgacagacgg
     9901 tagtttccgt tgcccagatt ggtaatggaa actaccgtct gtcggatgtg aatcgcgata
     9961 c
//
LOCUS       DAOPWB010000114         9951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6841_length_9951_cov_4.477264, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000114 DAOPWB010000000
VERSION     DAOPWB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6841_length_9951_cov_4.477264"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            418..1617
                     /locus_tag="PKN92_10015"
     CDS             418..1617
                     /locus_tag="PKN92_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49170.1"
                     /translation="MSLNFSGGSTAKSHQYSNYFSVDQHYFPQINQSTIKRDPESWLR
                     TYPHETFVEMLRQMERILARQERRSLWIEGAYGTGKSQCAYALKKILEVPEEELRNKL
                     YYDPNFDVSYDDILSLLNRCGDQFCSRNKVICLGNHPGKSDSQDPEGAMKDALLASFA
                     VNMQTEISRGIGPGEQISSLSPEAKRVNPHRKFLNVLHATLIKAGLYKNTSLPIFKAM
                     PSPTPVETPIPQWKDRTFFTRKCLYPVQPILYHGLRNEHKRSAQFGQKVHLYLDVSYS
                     MCDNLPWIVSALAPLEQAGWCRIFLFSTRVFPAPKEGIAQEELKTTGGTDISCVLQHI
                     VEWEPRTRPAKIIILTDGYFRVPIGTLMDQFKNTKVKIFGAITRNGSKCAMEAIAVYT
                     VLMPEYR"
     gene            1725..4121
                     /locus_tag="PKN92_10020"
     CDS             1725..4121
                     /locus_tag="PKN92_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNZ49171.1"
                     /translation="MADDVFVLSNKFDLYIPTQCRCGESLPESLRKRVLDQVKKQMAQ
                     WFGGVTSSDAQGGWVLQSGELAEEAVTIIYSNVSDSQKEEYLEGFVALAQSIADELAQ
                     EAIAYAINNNMSFAIGDADKPCLHQKTGGFKKPHALSADIAWMLSLEAMLRALCHNPQ
                     DKYQAAVKERVRDLLCHALGYSYADEPVHLTDKDGKLKPLFVPGSRPCVVAKGGDAFQ
                     IILIQLKDERLLLTEQRQIIDRTLKDNIGLRALFIVTDHKGRQWHLVNVPEISTTGRL
                     ILRRFRIEYADQPMRTLVERITYISLGDDAIRRRDDKIKEAHDRAFDVQHVTEAFFRD
                     YKRVFDQLQEGLFKQTRDKQWAHDYTLQFLNRIMFIYFIQKKRWLNNDPEFFYNFWHE
                     YRLCDQPENSFVKDWLHILFFEAFNNVFTDNCPYISKETRNAMAEAPYLNGGLFTRNE
                     LDEKHALKCQINDDAMEDILRFLQGYNFTIGEDTPLDQEVAVDAEMLGNVYQSLVNVS
                     DLNAGKEEVDSQKMAGIFYTPRIEIDMMCRLSLVDYLSNHLGAERKPLIYDFVFADTE
                     EKKQLVGQVLTEQNLWKEIDTLLQNVSAVDPACGSGAFLIGMLQVLDELLQRSGYAVG
                     RAETPYERKQQIIGRSLYGVDVMPWAVDVAELRLWLQLVVETELSVPERKMKPLLPNL
                     NFKIRQGDSLIQMVGNIDFSYMRSFSSLSASLKGRLRELKGDKLRFFNNQFQNKQQTR
                     NSIDKKERDIFDDILAERQHDITNKLKDLRTAIKPIAMLDGSRECPLSPKMPKKLNWR
                     "
     gene            4112..5791
                     /locus_tag="PKN92_10025"
     CDS             4112..5791
                     /locus_tag="PKN92_10025"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNZ49172.1"
                     /translation="MEIASLETEKKQLSEAIHSLRKTKKTPFVWDIAFVEIFEDSKKG
                     FDIVVGNPPYVRQELIADPLLSQAKQTTAENKKYKEKLIRSVYALWPSFFGVNPEKPD
                     RKLDQKNDLYCYFFFHGLARLNTKGSFIFITSNSWLDVGYGKDLQEFLLTQSHVKMII
                     DNKSKRSFKSADVNTVIALLGAPEKQKRSPRDKTARFLMFKEPFDAVDLLESFKAAEK
                     AKERTTLDQCRIFPIDQQTLWEQGSDALSADAPSGGKDKKTLTTLLHSAKYIGNKWGG
                     KYLRAPDIYWTLLEKGKGKLFSLGSVAEVRRGITSGANNFFYLSQETADKWGIEKEFL
                     KPIILSPRECPGTLVTKDTLNFQAFFCNKDETSLKGSNALRYIAWGESQGFHERPSCK
                     TRTLWYSLPDRKWARILWPMIHNDRLGVYWNKSRIAVDHNLFEIMSANDYVVWGSLAW
                     TGQVIFRELHGRANLGQGALKTEGTDIKTLYAIDSKDKGLTEVFRHVLSDLSDLNIDA
                     VETEVKKSERIKLDNILSDYLKLSRGERDAVYEAAIELIDLRLQKASSLNA"
     gene            5879..9193
                     /locus_tag="PKN92_10030"
     CDS             5879..9193
                     /locus_tag="PKN92_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNZ49173.1"
                     /translation="MSDIIDNCRTKLVDTINAILPTSEAVDFAVGYLFLSGLGAIEES
                     LDHPQRLRFLIGNATDRQTLEHFAEAFHRVDEAQQHNQAARYRNASEARELGDGAVEE
                     MRDALALMDQGDEDESVLQKVVRLIETGRLSIRVYTRGRLHAKAYLFHYPEDGRYEKG
                     MGIVGSSNLSLAGLTHNTELNVAVHGNENHEQLSVWFEERWAESLDLTQALLEEIRLS
                     WLLAEATPYDIYMKSLHALVADRLEGPVDDGTLWDDALTQSLADFQKEAVKRGVQIIR
                     DQGGVFISDVVGLGKSFIGAAILQHFRRTEGCKALIICPKALQESWEEFSHQFDLGAR
                     VIPMSRLQERRNELSHAKYEEYDFLLVDESHHFRHASSQRYDALQTYLMRRQRKLCLL
                     TATPRNKSAWDIYHQIKLFHPGDQTFLPVDPPNLKRYFKLVEEGSRSLQSLLKPLLVR
                     RTRRHILRWYGCAEDSGRPLRELDESDAADYLNGAKRAWVQVGAIRNYFPQRRLETLG
                     YNIDATYAGIYDSLRFRLGGNLKEGEEATGERLSYARFGLWHYVLPIMQKAAPYQDLQ
                     RVGANLRGLMRVMLFKRLESSVYAFLKTLERMIVVHENFLRCLDKGIIPAGDDAQRLL
                     KGSDALDEEELLDLMAQISSKYKIEDFDASRLYADVSADLELLRNMCSDLAGITPEKD
                     SKLQVLKEKLRDGIPRASGKVLLFTQFADTAQYIYEAINPGGADPAIAVIYGTDQSKI
                     RVAARFAPKANAFVHRPAGDPDIRILIATDVMSEGLNLQDGDVIVNYDLHWNPVRLIQ
                     RFGRIDRIGTENEEVWGFNFLPERALDENLGLTDRLKARIQEIHDSIGEDAAILDKEE
                     RINEESMFAIYQGEGHQMTLQEEEEDGVPDLTEAEAFFRRMQEEYPEEYERIRNLRGG
                     IRSSRALFSGRGSYVFCQAGQFQQLYLVDGKGKIVSRDMTEALLRIRCPREEPRALLP
                     LDHNARIMDVKALFVEEMNQRLAEQKSLYTASPAQAYVLRELRAYSGRTDDDDVKRRC
                     FAMENAFQGILPPALRENMNVLRRSAVTGDTLLRAVVELYHAFDLASMQRPGEKNGTS
                     RTIEDIPRIVCSEAFV"
     gene            9668..9865
                     /locus_tag="PKN92_10035"
     CDS             9668..9865
                     /locus_tag="PKN92_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49174.1"
                     /translation="MRTYLVERREQVSGGAFNEWHQVGIALDSELTLTEQPRNVQMEY
                     RIIAVNNGGNSLPSNTAAVVL"
BASE COUNT         2587 a         2370 c         2584 g         2410 t
ORIGIN      
        1 cgcaatgtct ccttttttgt ttctcaataa ttgcgtttcg aatttgaacc cgcgtagtga
       61 aatcatatgc ccggcttgtt ttgactcccc tcggcacgaa ccttgacttt ggtcatgtta
      121 cgggtgccgg ggatcttgtt cgcacgaagt gccggatcag tgatcttcgt gttgtgttgt
      181 acggcttatt caagtttgcg gagcagtgtg gcgattacaa ggaattcacg ctcagcctgt
      241 tgttgaatga cagtatagag cgggacggtt tgagtccttc gcggattttc ggtcttgacc
      301 gtgaggagat gcagtcgtgc ctgcagggcc tttccgcgaa acatcctgat tttctccatg
      361 cgagtttcac ccacgatctt gacaagatag cactttcaaa ggataagaca tcggaggatg
      421 tccttgaact tttcaggagg gagtactgcc aagagccatc agtacagtaa ttatttttca
      481 gtcgaccagc actattttcc gcagatcaac cagtcgacga tcaagaggga tccggagagt
      541 tggctccgca cctatccgca tgagactttc gtggagatgc tccgtcagat ggaacgtatt
      601 ctcgcacgac aggaaaggcg ttctctgtgg atagaagggg cctatggcac ggggaaatcg
      661 caatgtgcct atgcgttgaa aaaaattctt gaagtgccgg aggaggagct gcgcaataaa
      721 ctgtactacg atccaaactt cgatgtgagt tatgacgata tcctctccct gctcaaccgg
      781 tgtggcgatc aattctgttc ccgcaataaa gtcatttgtc ttggaaacca tccaggaaag
      841 agtgattccc aggatccgga aggggccatg aaagatgccc tgcttgcctc ctttgcagtc
      901 aacatgcaaa cagagatatc ccggggcatt ggccctggcg aacaaatatc ttcactctct
      961 cctgaagcga aaagagtaaa cccccaccgt aaatttctca atgtgctcca tgccacactc
     1021 atcaaagcag gcctctataa aaataccagt ctgcccattt tcaaagccat gccaagtccc
     1081 acccccgtgg aaacccccat tcctcaatgg aaagatcgaa ccttcttcac cagaaaatgc
     1141 ctgtaccccg ttcaacccat tctctatcac gggcttcgca atgagcacaa aagaagcgct
     1201 cagttcggtc aaaaggtgca cctttatctc gatgtcagct actccatgtg tgacaacctt
     1261 ccctggattg tcagtgctct ggcacctctg gaacaggctg ggtggtgtcg catatttctt
     1321 ttcagcacac gggtctttcc cgctcccaaa gaaggcattg cacaagaaga gctcaaaaca
     1381 accgggggaa cagacatctc atgtgtgttg caacatattg tcgaatggga gccgaggaca
     1441 cgtccggcaa aaatcataat tcttaccgac ggatacttcc gcgtgcccat cggaacgctc
     1501 atggatcagt tcaagaacac caaagttaag atttttgggg ctattacacg aaatggcagt
     1561 aaatgtgcaa tggaggcaat cgccgtatac acagtcttga tgcccgaata ccgatgagcg
     1621 caaagagatg gaagccattc aaaaaatcaa aacccaacac tttctttttt cgcacccgac
     1681 aaggcataat acacaaagcc catctatagg aggagtttca taccatggcc gatgatgtct
     1741 ttgtactgtc caataaattc gatctctata ttcctactca gtgtcgatgc ggggaatcac
     1801 ttcctgaaag cctgagaaaa agagttctgg atcaggtaaa gaaacaaatg gctcagtggt
     1861 tcggcggtgt cacctcgagt gatgctcagg gcggttgggt gttgcaaagc ggagaactgg
     1921 ccgaagaagc ggtaaccatt atttacagca atgtaagtga cagccagaag gaagaatatc
     1981 tggaaggctt tgttgcactg gcgcaatcca ttgccgacga actggctcag gaagccatag
     2041 cttatgccat caacaacaac atgtcgtttg ccatagggga tgctgacaag ccttgcctgc
     2101 accagaagac aggcggtttc aaaaagcccc atgccctgag cgccgacatc gcctggatgc
     2161 tgtccctcga agctatgctg agagcgctgt gtcacaatcc tcaagacaag tatcaggcag
     2221 cagtgaaaga gcgtgtccgt gatcttctgt gccatgcatt gggctattct tatgccgatg
     2281 agccggtgca tttgacagac aaggacggga aattgaaacc gctctttgtc cccggatccc
     2341 gcccgtgcgt cgttgctaaa gggggcgatg ccttccagat cattttaatt caactgaagg
     2401 atgaacgact gcttctgacc gaacaacgac agatcattga ccgtaccctc aaagataaca
     2461 taggactccg ggcactcttt attgtcactg atcacaaagg caggcaatgg caccttgtca
     2521 atgtgccgga aatcagtacc acaggccgcc ttattctccg gcgctttcgt attgaatatg
     2581 cggatcagcc catgcgtacg cttgtcgaac ggattactta tatttccctt ggcgatgatg
     2641 ccatcaggcg ccgtgatgat aagatcaagg aagctcatga ccgtgccttt gacgtacagc
     2701 atgtcaccga ggccttcttt cgggattata aacgtgtttt tgatcagttg caggaaggac
     2761 ttttcaagca aacacgggac aagcaatggg cacacgatta taccctgcag tttctgaacc
     2821 gcatcatgtt catttatttt attcagaaaa aacgctggct caacaatgac cctgaattct
     2881 tctataattt ctggcatgaa taccggctct gtgatcagcc ggaaaatagt tttgttaaag
     2941 actggcttca cattcttttc tttgaagcat tcaacaatgt attcacagac aactgcccct
     3001 atatctccaa agaaaccaga aatgccatgg cggaggcgcc ttatctcaat ggcggtctct
     3061 tcacccgaaa cgagctggat gagaaacatg ctctgaaatg ccagatcaat gacgatgcca
     3121 tggaagacat tcttcgcttt ctacaaggct ataatttcac tatcggcgaa gatacacctc
     3181 tggatcagga agtggcggta gatgctgaga tgctgggcaa cgtataccag agccttgtca
     3241 atgtatctga tttgaacgct ggcaaagagg aagtggacag tcaaaaaatg gcaggcatct
     3301 tttatacgcc tcgcatagaa atagacatga tgtgtcgcct ttctctggtt gattacctgt
     3361 ccaaccatct tggcgccgaa cgcaagcctt tgatctacga ttttgtcttt gccgatacag
     3421 aagaaaagaa acagctggtt ggccaagtgc tgactgaaca aaacctgtgg aaggaaatcg
     3481 acacccttct tcaaaatgtt tccgccgttg atcctgcttg tggatccggt gcttttctca
     3541 tcggcatgtt gcaggtgctc gacgagttgt tgcagaggag cggctatgcc gttggcaggg
     3601 cagagacccc ctatgaaaga aaacagcaga ttatcggtcg ctctctctac ggggtggatg
     3661 taatgccttg ggcggtggat gttgctgaac tgagactctg gcttcagttg gtcgttgaaa
     3721 cggaattgtc tgtaccggaa agaaagatga aacccttgtt gcccaatctg aacttcaaaa
     3781 tccgccaggg cgacagtctg attcaaatgg ttggaaatat cgatttttca tatatgcgca
     3841 gtttttcttc tctgagtgcc tctctcaaag gaagattgcg ggaactcaaa ggtgacaaac
     3901 tccggttttt caacaaccag tttcaaaaca aacaacagac tcgcaacagc atcgacaaaa
     3961 aagaacggga tatctttgac gatattctcg cagaacgaca gcatgacatc accaacaaat
     4021 tgaaagattt gcgcactgcc ataaaaccaa ttgccatgct ggacggaagt cgcgaatgcc
     4081 ccttatcgcc taagatgcca aagaagttga attggagata gcatctctcg aaacagaaaa
     4141 aaaacaactt tccgaagcaa tacattcgct tcgaaaaact aaaaaaacgc cctttgtttg
     4201 ggacattgct tttgtggaaa ttttcgagga cagcaaaaaa ggcttcgaca ttgttgtagg
     4261 gaatccgccc tatgtgcgtc aggaactgat agccgacccc ctcctttccc aagcaaaaca
     4321 aacaacagcg gaaaataaaa aatataaaga aaagcttatc cgttccgtct atgccctctg
     4381 gccttccttc ttcggggtaa atccggaaaa gcctgacaga aagctggatc agaagaatga
     4441 cctgtattgt tatttctttt tccacgggct ggctcgcttg aataccaaag gctcctttat
     4501 tttcatcacc tccaactcct ggctcgatgt gggatatggc aaagacctgc aggaatttct
     4561 gttgacccag agtcatgtga agatgattat tgacaacaag agcaaacgca gctttaaatc
     4621 tgccgatgtc aatacggtca tcgctctgct gggtgctccc gaaaaacaaa aaagatcccc
     4681 acgcgataaa acagcacgct ttctcatgtt taaagagccc ttcgatgcag ttgatctgct
     4741 ggaaagtttc aaggctgcag aaaaagccaa agagcgcacc acgctcgatc aatgtcgcat
     4801 ttttcccatc gaccagcaaa ccctctggga gcagggaagt gatgctcttt ccgctgacgc
     4861 accgtcggga ggcaaagaca aaaagactct tacaacttta ttgcacagtg caaaatacat
     4921 cggcaataag tggggtggaa aatatctgcg cgcgcccgat atctactgga cgcttttgga
     4981 aaaaggaaaa ggtaaattgt tttctctcgg atctgtcgcc gaggtgcgac gtggcataac
     5041 cagtggggcc aataactttt tctatcttag tcaagaaact gcagataagt ggggtattga
     5101 aaaagaattt ctgaagccta ttattttaag ccccagagaa tgcccgggaa cgttggtgac
     5161 caaggacacg ttgaatttcc aggctttctt ctgtaataaa gatgaaacgt ctttgaaagg
     5221 ctcaaatgct ttgcgatata tcgcttgggg tgaaagtcag ggctttcatg aaagacccag
     5281 ttgtaagact cgtactcttt ggtattcctt acccgacaga aaatgggcac gtattttatg
     5341 gcctatgatt cataatgatc gtttgggagt gtattggaat aaaagccgca ttgctgtcga
     5401 ccataactta tttgaaatta tgagcgccaa tgactatgta gtgtggggaa gtctggcctg
     5461 gactggacaa gtgatttttc gtgaattaca tggcagagct aatttagggc aaggtgcctt
     5521 aaaaacggaa gggacagaca ttaagactct atatgcaatt gactccaaag ataaaggact
     5581 gacggaagtc tttcgtcatg tgcttagcga tctctctgat ctgaatatag acgctgtcga
     5641 aactgaagta aaaaaatctg agcgcattaa attggacaat atccttagtg attatttgaa
     5701 gctgagtcgt ggagaacggg acgccgttta cgaagcggct atagaattga ttgatttacg
     5761 tctgcaaaag gcttccagtt tgaatgcata atgcgcatgt gtagaaccat tcaacttgtt
     5821 cataatgata gagaccgcaa cggtatatgt tttatagata ttgaaaggtc ctcttttcat
     5881 gtccgatatt attgacaatt gcaggacaaa gcttgttgac acgatcaatg cgattcttcc
     5941 gacttcggaa gcagtcgatt ttgcggtcgg ctatctgttc ctttcggggt tgggcgccat
     6001 agaagaatcg ttggatcatc cgcaacggct ccgttttctc ataggcaatg ccacggaccg
     6061 gcagacgctg gagcattttg ccgaggcctt tcaccgggtg gacgaggcac agcaacataa
     6121 tcaggcggct cgctatcgca acgcctctga ggcgcgggag ttgggcgacg gtgctgtaga
     6181 agagatgcgc gatgctctgg cgcttatgga tcagggcgat gaagacgagt cagtgttgca
     6241 gaaagtggtg cgccttatcg aaacaggacg cctgtccatc cgtgtgtata cccgtggccg
     6301 gcttcatgcc aaggcctacc tctttcatta ccctgaggac ggacgctatg agaaggggat
     6361 gggtattgtg ggctccagca atctgagtct cgccggcttg acccacaaca cggaactgaa
     6421 tgtggccgtt catggcaatg aaaaccacga acagttatcg gtctggtttg aagagcgttg
     6481 ggctgagagt ctggatctca cccaggcatt attggaagag attcgtctga gctggcttct
     6541 cgcggaagcc acgccctacg atatttatat gaagagtctt cacgctctgg tcgccgaccg
     6601 gctggaagga cctgtggatg acggaaccct gtgggatgat gcgctcacgc aaagccttgc
     6661 cgactttcag aaagaggcgg tcaagcgcgg tgtgcagatt atccgggatc agggaggcgt
     6721 gtttatttcc gacgtagtcg gtctgggcaa gagttttatc ggcgcggcga ttcttcagca
     6781 tttccgccgg acggagggtt gtaaggcgct cattatctgc ccgaaagcgc tgcaggaaag
     6841 ctgggaggag ttcagccatc agtttgatct tggggcgcgg gtgatcccca tgagccgcct
     6901 gcaggaacgg cgcaatgagc tgtcccatgc caaatacgaa gagtatgatt ttttgctggt
     6961 tgacgaaagc caccacttcc gccatgccag ttctcagcgc tatgatgccc tgcagactta
     7021 tctgatgcgg cgccaacgca aactttgcct gctcacggca acaccgcgca acaaaagcgc
     7081 ctgggatatt tatcatcaga tcaaactttt tcatccgggc gaccagactt ttctgccggt
     7141 ggatcctccg aatctgaagc ggtatttcaa actggtggag gaagggagcc gttcgctgca
     7201 gtctctcctg aagccgctgc tggtccgccg tacccgccgg cacatcttac gctggtatgg
     7261 ctgtgcggaa gacagcggcc gtcctttgcg ggaactggac gaatcggatg cggcggatta
     7321 tctgaacggc gccaaacggg cgtgggttca ggtgggggcg atccgcaact atttcccgca
     7381 gcggcgtctg gaaacactgg gctataacat cgatgcgacc tatgccggga tctatgactc
     7441 actccggttt cgcctgggcg gaaacttaaa agaaggagaa gaggcgacag gggagcgcct
     7501 gagctatgca cgcttcggtc tgtggcatta tgtcctgccc attatgcaaa aagcggcgcc
     7561 gtatcaggat ttacagcggg tcggcgcgaa tcttcgcggg ctcatgcggg tgatgctgtt
     7621 caagcggctg gaatcaagtg tttatgcctt tctgaagacg ctggagcgca tgattgtggt
     7681 tcatgagaat tttctgcgct gtctggataa gggcattatt ccggcgggcg atgatgcgca
     7741 gcgcctgctg aaaggctctg atgcgcttga cgaagaagaa ctgctggatc tcatggcaca
     7801 gatcagcagc aaatacaaga ttgaagattt tgatgcatcc cggctttatg cggatgtcag
     7861 cgcggatctg gaactgctga gaaacatgtg ttccgatctt gccggtatta ccccggaaaa
     7921 agacagtaaa ctgcaggtgc tgaaagaaaa gctccgcgac ggaatccccc gggcgtcggg
     7981 caaagtattg ctgttcaccc agttcgccga tacggcacaa tatatctatg aggcgatcaa
     8041 tcccggtggt gcagatccag ccattgcagt catctacggg acggaccaga gtaaaatccg
     8101 ggtggcagcc cggttcgctc cgaaagcgaa cgcttttgtg catcgcccgg caggagaccc
     8161 ggatatccgc atactcatcg ccaccgatgt tatgtccgaa ggattgaatc tgcaggatgg
     8221 agacgtgatt gtgaattacg atctccattg gaaccctgtg cgattgattc agcgcttcgg
     8281 gagaatcgac cgtatcggaa cggaaaacga agaggtatgg ggttttaatt ttttgcccga
     8341 acgggcattg gatgaaaatc tgggactgac cgaccggctc aaagcccgga ttcaggaaat
     8401 acatgattcc atcggagaag acgccgccat tctggataag gaagagcgta tcaacgagga
     8461 atccatgttc gctatttatc agggagaagg gcaccagatg accctgcagg aggaagagga
     8521 agacggtgtg cccgatctga cggaggcaga ggcttttttc cggcgcatgc aggaagagta
     8581 tccggaagaa tatgaacgca tccgcaactt gcgaggcgga atcagaagca gccgagccct
     8641 cttttccggg cgcggcagtt atgtgttctg tcaggcgggg cagtttcagc agctgtatct
     8701 ggtggacggg aaagggaaaa tcgtgtcccg ggatatgacc gaggcattgc tccgtatccg
     8761 ctgtccccgt gaagaaccgc gcgcactctt gccgctggat cacaacgcac ggattatgga
     8821 tgtgaaagcg ctgtttgttg aggaaatgaa ccaacggctg gcggaacaga agtccctgta
     8881 tacggcatca ccggcacagg cttatgtgct ccgtgagctg cgtgcttatt cggggaggac
     8941 ggacgatgac gatgtgaagc ggcgctgctt tgccatggaa aacgcatttc agggcatcct
     9001 gcccccggca ctcagagaaa acatgaatgt gcttcgccga agcgctgtta ccggcgatac
     9061 gttactgaga gctgttgtgg agctgtatca cgcctttgat ttagcctcga tgcagcgccc
     9121 gggagaaaag aacgggactt cacgtaccat tgaagacata cccagaattg tctgcagcga
     9181 ggcatttgtg taggagtaac aggcagcttt caaagcctgt tgcttgaatt caagcattgg
     9241 ctttaattgg cttgataagc aactgaaaat gccacaaaat atttggtgcg ggggatacgc
     9301 tttatgcctg tccggtctgt ttcccccgct gcccccccgg tgccaccagg gtatagaagg
     9361 gtatcaagtc tccaaagtct gaagtctttt tagagaggag gggacttctc ttctttcaga
     9421 gcacaggctg ttgaaccacg tttcgctccg gcagggtatg tgactcggat cacgcttgtt
     9481 tcagactttc cgccagcaga gtgatgattt cttcctttgt aagcccctgg agaagtcctt
     9541 tcaaaagtgc ctgttttgtg tcatcagggg taacgcaagg ggtaacacaa ggctcagacc
     9601 cattataagt gcttgatttt attggctttg ccaccgcagg cgtgcggact tttaatccgg
     9661 tggccgcgtg cgcacgtatc ttgtcgaacg gcgtgaacaa gtctcaggcg gcgcgttcaa
     9721 cgaatggcat caggtcggca tcgcgctgga cagcgaactc acgttgacgg agcaaccgcg
     9781 caacgtccaa atggaatacc gcatcatcgc cgtgaacaac ggcggcaaca gcttgcccag
     9841 caacacggct gcggtcgtgc tgtgaagaac tcaacgcaga gttgcggaga acgccaagaa
     9901 acgaagagaa tataaacatg actcttgaac aaaatgaaat gggatttctc t
//
LOCUS       DAOPWB010000115         9808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_6975_length_9808_cov_6.098739, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000115 DAOPWB010000000
VERSION     DAOPWB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6975_length_9808_cov_6.098739"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="PKN92_10040"
     CDS             <1..1484
                     /locus_tag="PKN92_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49175.1"
                     /translation="YTPKEGMVSLQPLSAPLLIEGSPLEEYALTASFALSITPPLSAG
                     VTRSRLQLHLSNPFTGSLEGMLTLSSGTRYAFGSSAVPVIIGPGKSFSLTRHLQGLSF
                     QGAGASFTMAFNRPGTLPQSRDFFFSVAPHEKGRLYTDAALSGMPVAVHRKDLGALLI
                     WCDRRGHVAACSTGSPQMQWEKRFRGTFSKAPVLLKDRQGNNLIAFANENNRVRLINP
                     GGTETAVLFPGGTSVSALKVVERADGGDILALLLDRKELVFYVEGTRFIGRKQVSGHI
                     AHLSSESPVPQSVLCVVAEEKKGDITHEMQCLSVTGETLWSQKIESAPALSPRIIKRD
                     VDTDPVICFPDSRGRVCFFEGRDGSPSGSMQLEGMHNPDDMLLLEGRSCLLVFGSATG
                     ITVYSAASDGSAAPLWQVDLPGITALAAWPDGMGIIAGTSDGGLYGFTEAGQCVWEDL
                     RGTGYITDLLFLPSETPRAFDLLAAGADGSLRFLEIQSSNSKN"
     gene            complement(1506..2930)
                     /locus_tag="PKN92_10045"
     CDS             complement(1506..2930)
                     /locus_tag="PKN92_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ49176.1"
                     /translation="MNSPMEQRYLERLNRYVTAMRNEKPDCIPIRPFAAEFVATYAGY
                     TCQQVTHDFPQCFDATCRTLADFDWDAAPGSMVYVWTGLTQALGLKYYGIPGIDFSPD
                     TAFQYIEPPEGHAFMREDEYDLLIANPTDFLYNVWLPRVSTEIPAPGAPATYRQNLAL
                     VKGAMAMMNYFNAFPAQGARMRAESGTVSAISGIFKAPFDILADKLRGYVGLVSDMLT
                     QPDKVLAACEALMPHLYHVAQSGADPEGHVPIGFWMHRGCVPFFSPDQFESHYWPTLK
                     PIIEALWARGHQTLFYGEGNWDYHLERFRELPDKSIVFHVDRTDMKKAHAVLGDKFCL
                     SGGIPNALLSYGTPDDVRAVCKEVIDTAGRDGGFIMDASAIIQNDARPDNIRAMTDFT
                     REYGQYGTAPVAAVPPRVNDGALKSGWPAEFPADTLPGGCIPWEEKRREIPEIPGDEA
                     LAKRIFQEIDGLGYLFIWHCLLSF"
     gene            complement(3021..4127)
                     /gene="mltG"
                     /locus_tag="PKN92_10050"
     CDS             complement(3021..4127)
                     /gene="mltG"
                     /locus_tag="PKN92_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNZ49177.1"
                     /translation="MDKIQNGQTEEAQKGDGGRFLHLLAFTALWAIVLVLAALITIAA
                     GAFLVYNHTVRNGVRGGEVVVEVLPGMTAKEIGVLLQDSGLLEHEGFFRLALYLEKEP
                     PSIRHGAYALYEGLSARELLALLQEGPSRHLLANQFRVTIPEGLSVAQASAICANPEA
                     FVQAAGNPQLIASLGVIVPSLEGFLMPNTYFFDEAPDGLTLVTRMVDQFRKEWDALAK
                     AFPGTEGRDKKEIVTIASLVEREAKTEEERPLVAAVIYNRLARQMPLQMDSTLQFALN
                     KYGQRMLNEDREVASPYNTYKYRGLPPGPICSPGISSLRAAINPAKENYLYFVSNADG
                     TTHTFSRTLAEHNQAVSRFRREIAPQRQTLQGQE"
     gene            complement(4172..4615)
                     /gene="ruvX"
                     /locus_tag="PKN92_10055"
     CDS             complement(4172..4615)
                     /gene="ruvX"
                     /locus_tag="PKN92_10055"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNZ49178.1"
                     /translation="MDTTGIIVGLDIGEARTGIARSDALQMLAFPVSVVRGKNEDEMV
                     REVSAAIKALSPVRVVAGLPLNQHGEPGAQARRVLAMIERLKEATGLPFVTQDERFST
                     AEANRVARDMETGGKKRKGKVDKIAATLILQTWLDREAALRRQQP"
     gene            4944..5969
                     /locus_tag="PKN92_10060"
     CDS             4944..5969
                     /locus_tag="PKN92_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ49179.1"
                     /translation="MRKIFTIIGGVIGILILLLLVYKVIMDKNYFADYDPKLPFNAQV
                     SETGIVDSTVDFFGITRPRRFVKVLFTMTTRPGETMPVLLTLPVERQGKLPAIVFLHG
                     IGQSKGFLEEICTPFNEAGFAMACFDQSMQGGRKVKGGWLAQATAFRKRPWKTVNDAR
                     RLIDYLQTHPEIDPERIYLIGASYGAITGSIVTSLDKRVRAADLVVGGANIPVMLSAP
                     FIKESVGNSALHWFGKQIVSSIMKPADPRHYVMRTAGTPVLMQNGSIDRLVSPQAGEE
                     LYRALGEPKEIRWYPCDHPGLGAKSEASIIVQILDEGLAWLLEKDKPFRKDGAPARTN
                     EIHQSAA"
     gene            6218..7000
                     /locus_tag="PKN92_10065"
     CDS             6218..7000
                     /locus_tag="PKN92_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49180.1"
                     /translation="MQFAIPDNQISKQERLICYYTLPRLLHPFTVGLLIAYMLCVAEA
                     LGALVFGMITDQPAWTSVGAWSLGGIIVLGLAAFTARALLNDWRRRRALALARKAPHT
                     LDDSDAPDLFAGHVLLSKPRPSSAPETVAQEIGTNRCYSAKALKGHSRWHIFYPEANR
                     VFDLSTVRVHGSYLFATRFLDLFEGEQKKARIALRKKLRGLRTRIDLCSPEAMEYQVC
                     DGCIRHNGRLVGRVYALRNRLYLDVESSHLNPGLVTCFLICW"
     gene            7116..7703
                     /locus_tag="PKN92_10070"
     CDS             7116..7703
                     /locus_tag="PKN92_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49181.1"
                     /translation="MALSKRQMKIVETLERRVELCRDFMNDWLLFNQILTSYPNPGVN
                     KAQLENQFLKIKSKLARTHRVLQQTLLDDYSLDGNTMNIVSGATNLEAMYSQSEVALK
                     KLQGEWHRAFISINETLGVLEDKKMRAENGEKVFVPSADMVHGGGGGGGMDAGTMKTI
                     LIIAAVIAAIAAVIIFDIGGIGTMYKEALGIRSGL"
     gene            7775..8080
                     /locus_tag="PKN92_10075"
     CDS             7775..8080
                     /locus_tag="PKN92_10075"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNZ49182.1"
                     /translation="MTRKDLALDLAHRLNLDIDDAQARVNAFFDVILDALEAGDRIEV
                     RGFGAFQIIERPAHKSRNPKTGQRLEVETRRTVKFKPGKQLQSSLREAGFKAQGKKG"
     gene            8241..9095
                     /gene="tatC"
                     /locus_tag="PKN92_10080"
     CDS             8241..9095
                     /gene="tatC"
                     /locus_tag="PKN92_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNZ49183.1"
                     /translation="MFGDDEKRMSFTEHLGELRTRLIRSCVALGIAVIACYVISDQIF
                     EMLAYPLRPLSQHGLLTSGAENAGSADAENGQSGAPATTSKAQEIRWTVLNPLEYILV
                     KFKIAGYGGVVLALPFILWQICAFIFPGLHANERRIVQILIAGCSSLAFIGVIVAYFG
                     VFPLVLPYLLEWIPQWVDVQLRLTETLSVIIKGLAGFGIAFQFPMVVLILVYMGLLTP
                     QTLKNYRKISIVGMAFLSAILTPPDPFSMLVMLFPLVILYESSIWASYLVIRRKKSRE
                     LVVTDKEN"
     gene            9101..9568
                     /locus_tag="PKN92_10085"
     CDS             9101..9568
                     /locus_tag="PKN92_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNZ49184.1"
                     /translation="MLGIGIGEMIIIAAIALMVIGPEKFPDFAKIAVRTIRDLRGYME
                     DIQSEVTKELAPVKKELEQLNKIKPEEYIDSLTQDKPEKVAGQSEIDTAGSADPTDRQ
                     HDGSGRTSSAVSAATSDETAQTPAAEDRESTEKEPPASPGEDFDNLAPPERLD"
     gene            9571..>9808
                     /locus_tag="PKN92_10090"
     CDS             9571..>9808
                     /locus_tag="PKN92_10090"
                     /inference="COORDINATES: protein motif:HMM:NF028174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HNZ49185.1"
                     /translation="MDTLEQHRLWSGEISAIANAFKKSQILFTAFESGIFELLEHPVS
                     AQEIATALNWSERGATMLLGGLLSLDLITLSDGKY"
BASE COUNT         2302 a         2651 c         2513 g         2342 t
ORIGIN      
        1 actatacacc caaagagggc atggtatcgt tgcagcctct ttccgcaccg cttcttattg
       61 agggcagtcc gctggaagaa tatgcattga ctgcctcttt tgctctttcc atcactccgc
      121 cgctgagcgc cggcgtaaca cgctcccggt tacagctgca tttatccaac cctttcacag
      181 gcagtctgga aggaatgctg acgctttctt ccggtacgcg ctacgctttc ggctcttcgg
      241 ctgttcccgt gattatcggt ccggggaaat ctttttcttt aacccggcat cttcagggac
      301 tttcttttca aggcgccggg gcatccttta ccatggcatt taaccggccg ggcacgcttc
      361 cgcagagccg tgattttttc ttttctgttg ccccccatga aaaaggccgt ctctacactg
      421 atgccgctct gtccggcatg cctgtagcgg ttcatagaaa agacctgggt gcactgctga
      481 tctggtgcga ccggagaggg cacgttgctg catgcagtac cggctcccct cagatgcagt
      541 gggaaaaacg gtttcgcggg actttttcca aagcgcccgt cctgctgaaa gaccggcagg
      601 gcaacaatct gattgccttt gccaatgaga acaaccgtgt acgcctgata aacccgggcg
      661 gtacagaaac agcagttctt tttccgggag gcacgtctgt atccgcactg aaagttgttg
      721 aaagagcgga cggcggggac atactggcgc ttctgctcga ccggaaagaa ctggtgtttt
      781 atgttgaagg cacacgtttt atcgggcgca aacaggtctc cggacatatt gcacacctgt
      841 ccagtgaatc ccctgtgccc cagtcagtac tttgtgtagt cgccgaggag aaaaagggtg
      901 atatcacgca tgagatgcag tgtctgtcgg tcacaggcga aacactttgg tcacaaaaga
      961 ttgaatcagc gcctgctctc agtccgcgca tcataaaaag agatgtggac accgatccgg
     1021 tgatctgctt tccggacagc cgcggcaggg tctgtttttt cgaaggccgg gacggaagcc
     1081 cctccggctc catgcagttg gaaggcatgc ataatcctga tgacatgctg ctgctggaag
     1141 ggagaagctg tctgctggtc tttgggtccg ccaccggaat tacggtttac tcagctgcgt
     1201 cggacggctc cgctgcacct ctctggcaag tggatcttcc cggcatcacc gcattggctg
     1261 cctggccgga cggcatgggc attattgccg gaacatcgga cggagggctt tacggcttta
     1321 ctgaagcggg acaatgcgtc tgggaagatc tgcgcggaac cggatacatc accgatctgc
     1381 tctttcttcc atcggaaaca cctcgggctt tcgaccttct tgccgccgga gccgatggca
     1441 gcctgcgctt cctcgaaata cagagcagca attccaagaa ttgagccgca ccgccgcccg
     1501 ctcttttaaa aagaaagcag gcaatgccat ataaagaggt agccgagtcc gtctatttcc
     1561 tgaaagatcc gcttcgccag ggcttcatct cctggaattt caggaatttc ccggcgcttc
     1621 tcctcccagg gaatacaccc gcccggaagc gtatccgccg gaaattccgc cggccatccg
     1681 ctcttgagag caccgtcgtt tacccgggga ggcacagccg ccacaggagc ggttccgtac
     1741 tgcccgtact cccgtgtaaa gtctgtcatg gcgcggatgt tgtcgggccg ggcatcattt
     1801 tggataattg cgcttgcgtc catgatgaac cctccgtctc tgcctgcggt atcaatcacc
     1861 tctttgcaca ctgcccggac atcatcgggc gtgccataag aaagcagtgc gttgggaatt
     1921 cctccgctca ggcagaattt atcgcccagt accgcatgcg cctttttcat atccgtacgg
     1981 tcaacatgaa aaacaatgct tttgtcgggg agttctctga atcgttccag atgatagtcc
     2041 cagttgcctt cgccataaaa aagcgtctgg tgcccccgtg cccatagtgc ctcaataatc
     2101 ggtttcaggg tgggccagta atgagactcg aactgatcgg gcgagaagaa cggcacacag
     2161 ccccggtgca tccagaagcc gatggggaca tgtccctccg gatcggctcc cgactgggca
     2221 acatgataca gatgaggcat cagcgcttca caggctgcca gcactttatc cggctgggtc
     2281 agcatgtcac tcacaagacc gacatacccg cgcagtttat cggcgagaat atcaaaaggt
     2341 gctttgaaaa taccggaaat agcggacaca gttcctgatt ctgcgcgcat ccgtgcgccc
     2401 tgcgcaggaa aggcgttgaa ataattcatc attgccatgg cgcctttgac caatgccaga
     2461 ttctgcctgt aggtggcagg tgcgccgggc gccggaattt ctgtcgacac ccggggcagc
     2521 cagacgttgt acaaaaaatc agtaggattg gcgataagca ggtcgtactc atcctcgcgc
     2581 ataaatgcat gtccttcagg gggctcaata tactgaaaag cggtatccgg tgaaaaatca
     2641 atgccgggaa taccgtaata tttcagaccg agcgcctgcg tcaaacctgt ccacacatag
     2701 accatgcttc caggcgccgc atcccagtcg aaatctgcca gcgtcctgca ggtggcatca
     2761 aaacactggg ggaagtcgtg ggttacctgc tggcaggtat acccggcata ggtcgccaca
     2821 aactctgcag caaaaggacg gatcggaata caatccggtt tttcattgcg catggcagtc
     2881 acatatcggt tcagccgctc cagataacgt tgttccatag gagaattcat aacgctgcct
     2941 accttcctct ttgctttgtt attttttcga tgcactttct gttaacgaaa aactgcatga
     3001 attcagcgat tccgatgtct ttattcctgg ccttgcaggg tctgacgctg gggagcgatc
     3061 tcccggcgga aacgcgacac cgcctgatta tgctctgcga gcgtcctgct gaacgtatgg
     3121 gtcgtaccgt ccgcattgga cacaaaatag agatagtttt cttttgccgg gttgatggcg
     3181 gcacgcagac tgctgatgcc ggggctgcaa ataggaccgg gcggcaaccc tctgtatttg
     3241 taagtgttgt acggggatgc cacttcccga tcttcgttca gcatgcgctg cccgtattta
     3301 tttaaggcaa actggagcgt cgaatccatc tgcagcggca tctgccgggc gaggcgatta
     3361 taaataacgg ccgccaccag aggtcgttct tcttctgttt ttgcttcccg ctcaaccagc
     3421 gaagcaatgg ttacgatttc ttttttatcc cgcccttccg tgccgggaaa tgcttttgcc
     3481 agggcatccc attctttcct gaactgatcc accatacgcg taaccagcgt caaaccgtcc
     3541 ggagcctcgt caaaaaagta agtgttgggc atcagaaagc cttccagtga cggcacaata
     3601 acacccagcg aagcgatgag ctgtggattt cctgccgcct gcacaaacgc ctcaggattc
     3661 gcacagatcg cagaagcctg cgccacagag agcccttcgg gaatggttac cctgaactga
     3721 ttcgccagca ggtgacggga gggaccctcc tgcagcaggg caagcagctc ccgggcggac
     3781 agcccctcat aaagggcata ggcaccatgc cgaatcgaag gtggttcctt ttcgagatac
     3841 agggcaagac gaaaaaagcc ttcatgctca agcagcccgg aatcctgcag cagcacgcca
     3901 atctctttgg cagtcattcc cggcagcact tcgaccacca cttccccgcc ccgtacaccg
     3961 ttccggacgg tatggttata cacgagaaaa gcaccggcgg caatggtaat cagagcggcg
     4021 agcaccagca caatcgccca aagggcagta aaggcaagca aatgcagaaa tcttccgcca
     4081 tcgccctttt gcgcttcctc tgtctgtccg ttctgaatct tatccataaa atgcctctgt
     4141 tggtctgatt gccccggcaa accatgccgg atcagggctg ctgtctgcgc agggcggctt
     4201 ctctgtcgag ccaggtctgg agaatcaggg tagcggctat tttgtccact ttccccttgc
     4261 gttttttccc gcctgtctcc atatcccggg cgacccggtt ggcttccgcc gtactgaatc
     4321 gctcgtcctg ggtcacaaaa ggaagtccgg tcgcctcttt cagccgctcg atcatcgcca
     4381 gcacccgccg ggcctgagca ccaggctcgc cgtgctgatt cagcggcagc ccggcaacaa
     4441 cccgcactgg agacaatgcc ttgatcgctg ccgacacttc ccgcaccatt tcatcttcat
     4501 tttttccacg aacgacagaa acaggaaatg cgagcatttg cagcgcatca cttcgggcaa
     4561 tccctgtccg cgcttcacca atatcaagac cgacaattat tcctgtagta tccatggcaa
     4621 gatcatagca aaagcaggaa ggggaagggc aactaaatca aggatatgtc ttcagcacaa
     4681 aaaggaaccg gagtgcttcg aggcgtccgg cgttcagaat cgtcttccaa aacttcctcc
     4741 gaattcatcc cgccgcatct ggacggcatc agtcgttttg gcgtatactt gaattatcgg
     4801 cacttttact aaaaccaaaa ggcttttttt aagtctatat gttggagttg tatcttgcag
     4861 aagcgttttc accagaatcg gggaaacact ctgcaaattt tactgtcttc gtttttttac
     4921 tttcaaactt cagggaattt gtcatgcgta aaattttcac gattattggc ggcgttatag
     4981 gcatcctcat tctcctgctc ctggtctata aggtcatcat ggacaaaaac tactttgccg
     5041 attacgaccc gaaactcccc tttaatgcgc aggtcagtga aacaggcatc gtagacagta
     5101 cagttgattt cttcggcatt acccgtccga gacgctttgt taaagtgctt tttaccatga
     5161 ccacgcgtcc gggagagacc atgcctgtgc tgcttacact gcctgtggaa cggcaaggaa
     5221 aactcccggc tattgtcttc cttcacggaa tcggacaaag caagggtttt cttgaagaga
     5281 tatgcacacc tttcaatgaa gccggttttg ccatggcatg ctttgatcaa tccatgcagg
     5341 gcggccggaa agtgaaaggc ggatggctgg cacaggcaac cgcattccgc aagcgcccct
     5401 ggaaaactgt caatgatgca cgaaggctga tcgactatct gcagacccat cctgaaattg
     5461 atccggaacg aatctatctg ataggtgcca gttatggagc cattacagga agcatcgtca
     5521 cttctttgga caaacgtgtc cgggcagccg acctggtagt cggcggagcc aatattcctg
     5581 tcatgctgag tgcaccgttt ataaaggaat cagtcggaaa ttcggcactg cactggtttg
     5641 gaaagcagat tgtttcctcc ataatgaaac cagccgaccc gagacattat gtcatgcgaa
     5701 cagccggtac ccctgtgctc atgcaaaacg gctctatcga caggcttgtc agccctcagg
     5761 caggcgaaga actctaccgg gctttgggcg aacctaaaga aatacgctgg tatccctgcg
     5821 atcatcccgg tctgggagcg aaatcggaag cttcaatcat tgtgcagatt ctggatgaag
     5881 gactggcgtg gctgttggag aaggataagc ccttccgcaa agacggggct cccgcccgga
     5941 caaatgagat acaccagtct gccgcctgac catacatagg aacaattgca gcgaagtgct
     6001 gtgtatgcat tgtaaaaata ccgtgtgccg ggtatactaa ggtcaaactg atgaacctgt
     6061 ggacaggagc gatacaagaa ggctttttca gttgatttcg cctgcaacca catgaataat
     6121 ccggcacttg cccgctttat tttttattct ccggtaaaaa cagggcagat agttttctga
     6181 ccggcagtgc agtatcgtct gcagataaac cttttctatg cagtttgcca tccccgacaa
     6241 ccaaatcagc aagcaggaac ggttgatctg ctactacacg cttccgagac tcctgcaccc
     6301 tttcactgtc gggcttctga tcgcctatat gctgtgtgtg gcggaagccc ttggtgcttt
     6361 ggtatttggc atgataacag atcagcctgc ctggacatct gtcggggcct ggtcgctggg
     6421 cggcattatt gttctcggac tggcggcttt taccgcccgg gcgctgctca atgactggcg
     6481 cagacgcaga gcgctcgctt tagcccggaa agctccgcat accctggacg acagtgacgc
     6541 gcccgatctt tttgccggac atgttttact gtcgaagcct cgtccttcgt ccgcaccgga
     6601 aacagtagcg caggaaatcg ggacgaatcg gtgttattcc gcaaaagccc tgaaaggcca
     6661 ttcgcgctgg catattttct atccggaggc gaatagggtt tttgatctct ctaccgtacg
     6721 ggtacacggc tcctacctct tcgccacccg cttcctcgat ctcttcgaag gcgaacagaa
     6781 aaaggcgagg atcgcgctgc gcaagaagct gcgtggcttg cgtacccgga tagatctctg
     6841 cagccccgag gcaatggaat atcaggtttg tgacggctgc atccgtcata acggcagact
     6901 ggtaggacgc gtttacgcac tcagaaaccg cctgtatctg gatgttgaaa gctcccattt
     6961 aaatccggga ctggtcacct gctttctgat ttgctggtaa cagccttccc gcggagcacg
     7021 ccgggcatgc aggtttggtt tttcagcctg ttctgtgcta aattattgat ttgtacggtc
     7081 taagattttc ttccattgag gaggcttttt aaactatggc acttagcaaa agacaaatga
     7141 agattgtgga gacactggag cgccgggtgg aattgtgccg ggattttatg aatgactggc
     7201 tccttttcaa ccagatcctt acttcctatc ccaatccggg cgtcaacaaa gcacaattgg
     7261 aaaaccagtt cctgaaaatt aaaagcaaac tggcacggac gcatcgtgta ctgcagcaga
     7321 cccttctgga tgattacagc ctggacggaa acacaatgaa tatcgtctca ggtgcgacga
     7381 atctggaagc gatgtactcc cagtctgaag tggccctgaa aaagcttcag ggtgaatggc
     7441 atcgcgcctt catttcgatt aatgaaacac tgggcgttct ggaagacaag aagatgcggg
     7501 cagaaaacgg tgaaaaagtt tttgtgccct cagcagacat ggtgcatggc ggtggtggcg
     7561 gcggcggtat ggacgccggc actatgaaga ccatcctgat cattgccgcc gtcattgccg
     7621 ctattgccgc tgtcattatc tttgatatcg gcggcattgg cacaatgtac aaggaagctc
     7681 tgggaatcag aagtggactg taacctctga tggcagatag atcttgccct cctcaaaaca
     7741 tgtatatttt ccatcagaaa acaggatata gaccgtgact cggaaagatc tggctcttga
     7801 tctggcgcac cgtttaaatc tggatataga cgacgcgcag gctcgagtaa acgctttctt
     7861 tgatgtaatt ctggatgctc tggaagcagg tgaccgcata gaagtccggg gcttcggtgc
     7921 gtttcaaatc atcgaacggc cggcccacaa aagccgcaat cccaaaaccg ggcaacgtct
     7981 ggaagttgag acacgccgga cagtgaaatt taagccgggc aaacaattgc agagcagcct
     8041 tcgcgaagca gggttcaagg cacagggaaa aaaaggttga tccggaaaat cctgacagga
     8101 aataacgccg tccgtccgac agggtcctgt ttcatgttcc cgctctcaca ggatttaggc
     8161 aaccgctttt tgttacaaag ataaacagca acagacgatc tcttgaactg accgcctgac
     8221 tacataagaa ggagtaacct gtgttcggag atgatgaaaa aaggatgagc tttaccgaac
     8281 atttgggtga gttgcgtacc cggttgattc gatcctgtgt tgcactcggt attgccgtga
     8341 tcgcctgtta tgtgatttcg gatcagattt tcgaaatgct tgcctatccg ctgcgtccgc
     8401 tcagccagca cggactgctc acttccggtg ctgaaaatgc cggatctgca gatgctgaaa
     8461 atggtcagtc cggtgcgcct gctacaactt ccaaggcaca agaaatacgc tggactgttc
     8521 tgaatccgct ggaatatatt ctggttaaat ttaaaattgc ggggtatgga ggcgtcgtcc
     8581 ttgccctacc cttcattcta tggcagattt gtgccttcat ttttcccggg ctgcatgcca
     8641 acgagcggcg catcgttcag attctcatcg caggctgcag ctctcttgcc tttattggcg
     8701 ttatcgttgc ctatttcggc gtatttcccc tcgtactgcc ctatctgctt gagtggattc
     8761 cgcaatgggt tgatgtgcag ctgcgcctca cagagacttt gtccgttatc ataaaagggc
     8821 tggcagggtt cggcatcgct ttccaattcc ccatggtcgt actgattctg gtgtacatgg
     8881 gacttctgac cccgcagacg ctcaaaaatt acaggaagat ctccatcgtt gggatggcct
     8941 ttctttccgc tattctcaca ccccccgacc ccttctccat gctcgtcatg ctcttcccgc
     9001 tggtcattct ctatgaatcc agcatatggg catcctacct tgttataaga cgcaaaaagt
     9061 cgcgggaact ggtcgtcaca gataaggaaa actgaccgtt atgcttggta tcggcatagg
     9121 tgaaatgatc attattgccg ccatcgccct gatggtgatc ggcccggaga aatttcctga
     9181 ttttgccaaa atcgcggttc gaaccattcg cgatctgcgg ggctatatgg aagacatcca
     9241 atccgaagtc accaaagaat tggcgcccgt taaaaaagaa ctggaacaac tcaataaaat
     9301 caaaccggaa gaatatattg actcgctgac gcaggacaaa cctgagaaag ttgccgggca
     9361 gtctgaaatc gacacggcgg gcagtgccga tcccacagac aggcagcacg acggatccgg
     9421 acggacatca tctgcagtca gtgcggcaac ttcagatgaa acagcgcaaa cacccgctgc
     9481 cgaagacagg gagtcaacag aaaaggagcc ccctgcatct cccggcgagg attttgacaa
     9541 tctggcaccg cccgaacgtt tggattaagc atggatactc tggaacagca tcgtctctgg
     9601 tcaggcgaga tcagcgctat cgccaatgct tttaaaaaat cacaaatcct ctttaccgcc
     9661 tttgagtccg gaatttttga gctgctggaa caccctgttt ccgctcagga aattgccaca
     9721 gcgttaaact ggagtgagcg cggcgcaacg atgctgctcg gcggtctgct gtctctggat
     9781 ctgatcacac tgtccgacgg aaaatacc
//
LOCUS       DAOPWB010000116         9747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7026_length_9747_cov_5.634131, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000116 DAOPWB010000000
VERSION     DAOPWB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7026_length_9747_cov_5.634131"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1420
                     /locus_tag="PKN92_10095"
     CDS             <1..1420
                     /locus_tag="PKN92_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49186.1"
                     /translation="AVFLTAKDMVAGALAAETPPKFSKLFDMPGFSLTIALPVLNGEQ
                     AEENILKFLSNLLTGIEVTEEQVAGLTLKKYEDHAAYFVTPTMFVISNNMDVLKSAAE
                     RAADPAVFQYGTTDCPPEFTDEGVMLLFVNRLMPLLDSAEQEMKELGPLADALSGVQM
                     DQMKAMCEDSKDTEPIVVSWHANKESAALKSKISTLAYPVVAVSQGDGKAAVWSQRLP
                     RNTMAFLSLILNDAIKKRIVEDYIGAIPEEVLKQSKASKGMQYLPNVMQLLGGEVTLG
                     LTGLDPLDAPSIFLIIELASAPTAQMFLGMAPQQDSGEPYKGIQIKQLTIPAPMPVYF
                     ALVESALILTNSDEGLKSFIDQAQSGTTSGFYDAVTPPLDANALIYHSFYLNPDFYTE
                     VVEPMASLIGMTLPEKYTDVAEEAARIVRDIRAGTSQDGEWLTGKLEIILKPAADGGE
                     AGNETPADTPDPDAAASDEADA"
     gene            1430..2281
                     /locus_tag="PKN92_10100"
     CDS             1430..2281
                     /locus_tag="PKN92_10100"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNZ49187.1"
                     /translation="MIRMAAPVCIFAAVLASAMAVAAEEQTAPRVLLFTKSAGFEHSV
                     IKETRRQPVSHAEGILRSITDEMGAVLESTKDGRRINKETLSSVDVVIFYTSGNLTEK
                     GNDGHPPVTPEGMNALFDWIRGGGGFIGLHAATDTFKSEGGATTAFTEMIGGEFVTHG
                     EQFKASVRKVSPDHAAVRALPDVWAVQDEWYMFARLNKEHMHVLALLETGGGGNPQKM
                     YQVPDYPIIWCRSYGDGRALYNGLGHREDVWEHPVFRQMLHDHILWAQGRGDAHTEPN
                     YAEVVPE"
     gene            2344..5493
                     /locus_tag="PKN92_10105"
     CDS             2344..5493
                     /locus_tag="PKN92_10105"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNZ49188.1"
                     /translation="MKAITVICGSVFSGRSKQIQALWAEKKEQALLITPTRRLAQLRE
                     EQLIRETNLPGLWNRRSRELSEYAALLLERSGCPVRMATRLERNRIVWLALKTLADQE
                     EIPEVSAGFVRHLLQVITSMKQNAVEVADLDRAIDNIREKHPFDRLVSKIYKAYQHEL
                     LQRELYDVPGLFWAVEQRCKEGLIALPEEEQWLLLDGFDDFTASQQRLLLALASLASG
                     MVIGLNYDAEAPDLEELYALQRRWLEKFQQITDVQIVRCPTAPEKSRSAYASRYLFSQ
                     HTPPPPAETLSANVTLMPAADACHEIESIGRTIKSLLLNDALRPAEIAVAFPDMEQQA
                     PLVEEIFTRFSIPVMMRTRLPLSRSRPAIFLLRLVKAVQEQSLQEFAPLLASPAFDRQ
                     DDSPQAVQSLVLLIRESNITGRLDESARALNYLQRLIEQKRDGNERSRLSQGCTPEAM
                     QLLLERLDQIREFTPRLPAEGTFRLYSESLSTFLDLPGLNRDTETEPGNQEAIAALRR
                     ILAEMASPFYENQPVTFSDYFALFRMALSEESVCGRGRSGVFCCSLTDLRLNAFPVVF
                     LGGLNEGALPGAAAINALYSDRDLARFRKEGLSMPGSSEHLQKQRLLFHHALCAAEKS
                     LFVGWRNQDQEDREILPSPFAVELRELFQKAVPPLTVDEPAPDCFIADLHQVASPEDF
                     RLSARYHQATIKSPSLCALLEPAQAMIEMEQTRATSKNFSVYDGMIESPELKADLLRR
                     FGEKHQFSVSQLEEYLKFPFSFFTKYVLKIKETRYADGEITPLLRGVILHYALKLFYE
                     VYRQKPANTLKNTSPEERSLLIQNSLDRAFEEQRFALSNIAPVVVALEKARLNLLLDQ
                     FIQEEAATEDSFVPWVLEYSFGTSSRPEQDPLSTALPFTYEVGEMQIMFAGIIDRIDK
                     DNLNRLRIIDYKSSSAPTKKEIKEGLSLQLSIYAAAAEASFPGCSSVEGWYIPLLKKK
                     PQEALFAGKESESLKRNANAVAMISKAICGIRAGCFPPLPHQSLNLDYFTLPQAARYE
                     AWRVAEKTDPFKENPTPVH"
     gene            complement(5538..6917)
                     /gene="argH"
                     /locus_tag="PKN92_10110"
     CDS             complement(5538..6917)
                     /gene="argH"
                     /locus_tag="PKN92_10110"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNZ49189.1"
                     /translation="MSKLWGGRFDGTTHSLMEKLGESVSFDHRLAPWDIEASRAHAKM
                     LAACGIITKEEGSLLDQGLAELAEEVRNGSVQWDSRLEDVHGNIEALLIKKVGDAGKK
                     LHTARSRNDQIATDMRLWTRDQADAIDAALHALQHAILHFAEEHVQVVLPGYTHLQPA
                     QPVLLAHHMLAYFEMFSRDRERFAQLRKRINVMPLGSAALAGTPHPIDRAYTAELLGF
                     EKLSANSMDAVSDRDYLIEFCGCASLIMMHLSRWSEEIILWNSPPFSFISLGDAFTTG
                     SSIMPQKKNPDAAELTRGKAARVFADHQALLVLLKGLPLTYNRDLQEDKEPVFHASDT
                     VQLCLSVFTAMLRTITVRRDVMERAVGDGFMEATDLADYLVYKGVPFREAHHIVGETV
                     RYCIGVGKTLTALSLDEFRRFCPEVEEDVFDALQIETILARRCQPGATAPAQVRVALV
                     EARKRLADI"
     gene            complement(7015..7188)
                     /locus_tag="PKN92_10115"
     CDS             complement(7015..7188)
                     /locus_tag="PKN92_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49190.1"
                     /translation="MKVQDFAYQVSLRTMELLENAQHYKITEANRKEILATILKELDT
                     LIQKSSAPVKKKK"
     gene            complement(7326..8033)
                     /gene="rplA"
                     /locus_tag="PKN92_10120"
     CDS             complement(7326..8033)
                     /gene="rplA"
                     /locus_tag="PKN92_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNZ49191.1"
                     /translation="MSHSSKRVKANRAKVDKFHFYTLSESVALAKENANAKFDETVEL
                     AFLLGVDPRHADQMVRGSVSLPHGTGKTVRVVVFCDQEDQMNAAKAAGAEEVGGEDLV
                     NKIQAGWTDFDAAVATPAMMRHVGRLGKILGPRGLMPSPKAGSVTADVGKAVEEIKAG
                     KIEYRVDKFANIQVPIGKVSFDAQMLEENAKSVIEAIMKARPSACKGTYLKGLAMSST
                     MGPGMRMDPAAITALFK"
     gene            complement(8110..8544)
                     /gene="rplK"
                     /locus_tag="PKN92_10125"
     CDS             complement(8110..8544)
                     /gene="rplK"
                     /locus_tag="PKN92_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNZ49192.1"
                     /translation="MAPKKKKIKAQVKLQVKAGQANPAPPVGPALGQHGLNIMDFCNQ
                     FNERTKEQMGLVVPVIITIYEDRSFSFITKTPPAAVLLKRSAKLAKASAEPNKTKVGS
                     VTDAQIQEIAELKMADLNAANIDTAKKMIAGTARSMGLRVEG"
     gene            complement(8562..9212)
                     /gene="nusG"
                     /locus_tag="PKN92_10130"
     CDS             complement(8562..9212)
                     /gene="nusG"
                     /locus_tag="PKN92_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNZ49193.1"
                     /translation="MADTTQNSTPYEEFELSPKEEEVEKGFSLNTPPEDGLNRRWYVL
                     HAYSGRETSVRDMLLAQAQQKNLEHLICNAFVPIEQVEVVRGGQKRVSKQKCFPGYVL
                     LQLPEHPETYPELWAIIRDTPSILGFIGSKNVPVPLEDEEVNQILEVVRGERERPKVK
                     ISFDKGERIRINEGPFANFFGNIEEIDSDRNILKVSVEIFDRQTTVEVEPWQIEQI"
     gene            complement(9224..9457)
                     /gene="secE"
                     /locus_tag="PKN92_10135"
     CDS             complement(9224..9457)
                     /gene="secE"
                     /locus_tag="PKN92_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNZ49194.1"
                     /translation="MAKQVSAPVKEPGIFARLQDFFDSVIAELKKVTWPTREDLMAST
                     KVTLFIIAIMAGVVFVYDRVFSIFIMLILKLAA"
     gene            complement(9524..9599)
                     /locus_tag="PKN92_10140"
     tRNA            complement(9524..9599)
                     /locus_tag="PKN92_10140"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9564..9566),aa:Trp,seq:cca)
BASE COUNT         2378 a         2577 c         2494 g         2298 t
ORIGIN      
        1 cgctgtattt ctgacagcta aagacatggt ggcaggtgct ctggcggcag aaacgcctcc
       61 gaaattttca aaactttttg atatgcccgg tttttcgctg accattgcgc tgcctgtgct
      121 caatggcgag caggccgaag agaatattct caaattcctc agtaatctcc tgaccggcat
      181 cgaagttaca gaagaacaag ttgccggact gacccttaaa aaatacgaag atcacgccgc
      241 ctactttgtc acgcctacca tgttcgtcat ctccaataac atggatgtgc tcaagagcgc
      301 tgcagaacgt gccgcagacc cggccgtgtt ccagtacggc accacggact gcccgccgga
      361 atttacagat gaaggcgtaa tgctcctgtt cgtgaaccgt cttatgcctc ttctggattc
      421 tgcggagcaa gagatgaaag aactcggtcc tcttgcagac gcactctcag gcgttcagat
      481 ggatcagatg aaagccatgt gcgaagacag caaggatacg gagcctattg ttgtcagctg
      541 gcatgcaaac aaggaatctg cagctttaaa atcgaaaatc agcaccctcg cctatcccgt
      601 tgtggcggtg tctcagggag acggaaaagc ggcagtatgg agccagcggc ttccccggaa
      661 cacaatggct tttctcagcc tgatactgaa tgatgccatc aaaaaacgga tcgttgagga
      721 ttatatcggt gccattcccg aggaagtgct gaagcagtcc aaggcttcca agggcatgca
      781 gtaccttccc aatgtcatgc agctgctggg cggagaagtt acgctcggac tcaccggtct
      841 tgatccgctg gacgctccct ccattttcct gatcattgaa cttgccagtg ccccgacagc
      901 gcagatgttt ctgggcatgg cgccgcagca ggacagcggc gagccctata agggcatcca
      961 aataaaacag ctcactatcc ccgctcccat gccggtgtat tttgcgctgg tggaaagcgc
     1021 actgattctg accaacagcg atgaagggtt gaaatcattc attgatcagg cgcagtcggg
     1081 cacaacaagc ggattttatg atgctgtgac accgcctctg gatgccaatg ccctgatcta
     1141 tcatagtttt tatctgaacc cggatttcta cacagaagtg gtcgaaccta tggcttcgct
     1201 cataggtatg acgcttccgg aaaaatatac agatgttgcc gaagaggctg cccggattgt
     1261 tcgtgacatt cgtgccggca ccagccaaga cggagagtgg ctcaccggaa aactcgagat
     1321 tattctcaag cccgctgcgg atggcggtga agccggaaac gaaacgcctg ctgatacgcc
     1381 ggatcccgat gctgcagctt ccgacgaagc ggacgcatag gaagccgtca tgatccgtat
     1441 ggctgctccc gtgtgcatct ttgcggctgt gctcgcttct gcaatggcag ttgcggcgga
     1501 agaacagact gctccgcggg ttcttctttt taccaaatct gccggatttg aacacagtgt
     1561 aatcaaagag acacggcggc agccggtcag tcacgctgaa ggcattttgc gctccattac
     1621 ggatgaaatg ggcgctgtgc tggagagcac taaagacggc agacgcatca acaaggaaac
     1681 gctgtcctct gtggatgtgg tcatttttta tacttccggg aatctgacag aaaaaggaaa
     1741 cgatggacac ccgcctgtca ctccggaagg catgaatgcg ctttttgact ggatccgcgg
     1801 cggcggaggc tttataggtc tgcacgccgc cacggatacc tttaagagtg aaggcggagc
     1861 aaccaccgcc tttacagaaa tgatcggcgg cgaatttgtc acacacggag agcagttcaa
     1921 ggcatccgtc agaaaagtca gccccgatca cgccgccgtc agagctctgc ccgatgtctg
     1981 ggcagtgcag gacgaatggt atatgttcgc ccggctgaac aaagagcata tgcatgtgct
     2041 tgcgcttctg gaaaccggag gtggaggcaa tccgcagaaa atgtaccagg tgcccgatta
     2101 tcccattata tggtgccgca gctacgggga cgggcgggct ctttacaacg gactgggaca
     2161 ccgtgaagat gtctgggaac acccggtctt cagacagatg ctccatgatc acatactctg
     2221 ggcgcagggt cggggcgatg cgcatacaga gccgaattat gcggaagtgg tgcctgaata
     2281 agaggatctg tcactatcag catgaatgac ggattcagat gccccgcaga agggtgaatg
     2341 cgcatgaaag caatcacagt catttgcggc tccgtttttt ccggaagaag caaacaaata
     2401 caggcgctgt gggcggaaaa aaaagagcag gctttgctga tcacgccgac gcgaaggctc
     2461 gctcagctac gcgaggaaca gcttatccgt gaaacaaatc tgccgggact ctggaaccga
     2521 cggtccagag aactgagcga atatgccgct ttgctgctgg aaagaagcgg gtgcccggtg
     2581 cgcatggcaa cgaggctgga gcgcaaccgt attgtgtggc ttgcactgaa aacgcttgcc
     2641 gatcaggaag aaatcccgga agtcagtgcg ggctttgtga ggcatctgct gcaggtcatc
     2701 acttccatga aacagaatgc cgtggaagtg gctgacctgg atcgcgccat tgataatatc
     2761 agggaaaagc acccctttga ccggcttgta tcgaaaattt acaaagcata tcagcatgaa
     2821 ttgctgcaac gggagctcta cgatgtgccc ggccttttct gggcagtgga gcagcgctgt
     2881 aaagaggggc tgatcgcatt gcctgaagaa gagcaatggc tgctgctgga cggcttcgat
     2941 gatttcactg cttcacagca gcggctgctg ctggcgctgg cttctcttgc ttcaggtatg
     3001 gttatcggac tgaattatga tgcggaagcc cctgatcttg aggaactgta tgcgctgcag
     3061 cgaagatggc tggagaaatt tcagcaaatc acggatgtgc aaatcgtccg ctgtcccact
     3121 gcgccggaaa aaagtcgcag cgcatatgca tcccgctacc ttttctctca gcacacccct
     3181 ccgccgcccg ccgaaaccct atccgcaaat gtgactttaa tgcctgccgc cgatgcctgc
     3241 cacgaaatag aatcaatcgg acggacaatc aaatcactgc tgctgaatga tgccctgcgg
     3301 cctgccgaga tagccgtcgc ctttcccgat atggaacagc aggcgccgct ggtagaagag
     3361 atctttaccc gcttttccat ccctgtgatg atgcggacac gcctgcctct ctcccgttcc
     3421 cgccccgcca tctttttact ccggctggta aaagccgttc aggaacagag cctgcaggag
     3481 tttgcacctt tacttgcctc acctgccttc gaccgccagg acgacagccc gcaggcagtg
     3541 cagtcactgg tgctgctgat ccgggagagc aacatcaccg gaaggcttga cgaaagcgcc
     3601 cgggcactga attatctgca gaggctgatt gagcagaaaa gagacggaaa cgaaagaagc
     3661 aggctttcac aggggtgcac tcccgaagcg atgcagctgc tgctggagcg gctggatcag
     3721 atacgggagt tcactccaag actgcccgct gaaggcactt tccgactgta ttctgaaagt
     3781 ctcagcacct ttctggatct gcccggatta aacagagaca cggaaactga acccgggaat
     3841 caggaagcta tcgccgcact gcggcgcatt ctagccgaga tggcgtctcc tttctatgaa
     3901 aatcagcccg tcactttttc tgattacttc gccctcttcc gcatggcact gtctgaagaa
     3961 tcggtttgcg ggcgcggcag aagcggtgtt ttttgctgca gcctcaccga tctacgactg
     4021 aatgcctttc ccgttgtgtt tttgggcgga ctgaatgaag gcgcattacc cggtgctgca
     4081 gcgattaatg ccctctattc agaccgggat ctcgcccgtt tccgaaaaga agggctttcc
     4141 atgccgggca gttcagagca tcttcagaaa cagcgcctcc ttttccatca tgctctttgc
     4201 gctgctgaaa aatctctttt cgttggctgg cgcaaccagg atcaggagga cagagagatc
     4261 cttcccagtc cttttgctgt cgaattaaga gaacttttcc agaaagcagt cccgccgctt
     4321 acagtcgatg agccagctcc ggattgcttt attgccgacc tgcatcaggt ggcatcaccg
     4381 gaagatttca ggctatctgc ccgctatcat caggcaacga tcaaaagccc gtcgctttgt
     4441 gcacttcttg agccggcgca ggctatgatc gaaatggagc agacccgggc cacgtcgaaa
     4501 aacttttcgg tatacgacgg catgattgaa tcgccggagc tgaaggcaga tctcctcagg
     4561 cgcttcggtg aaaaacacca gttcagcgtc agtcaactgg aagaatatct gaagtttccc
     4621 ttttcttttt tcacgaaata tgtactgaaa ataaaagaaa cccgatatgc ggacggagag
     4681 atcacgccgc ttcttcgcgg agtaattctg cattatgcgt tgaagctctt ttatgaagtt
     4741 tacaggcaga aaccggctaa tacactgaaa aatacaagtc cggaagaacg aagtctcctt
     4801 atccaaaaca gtctcgaccg cgcttttgaa gaacagcgct ttgcgctgtc aaacattgca
     4861 cccgttgttg ttgctctgga aaaagcgcgg ctgaatctgc tgctggatca gttcatccag
     4921 gaagaagcgg caacggaaga ttcatttgtc ccctgggtgc ttgaatacag tttcggcacg
     4981 tcctcccgcc cagaacagga tcctctttct acagccctgc cgtttactta cgaagtgggt
     5041 gagatgcaaa ttatgttcgc cggcattatc gatcggatag acaaagacaa tctgaataga
     5101 ctgcgaatca tcgactataa aagcagctcc gctccgacaa agaaagaaat caaggaaggg
     5161 ctctctttgc aactgagcat ctatgccgcg gccgcggagg cttcttttcc gggatgcagc
     5221 agcgtggaag ggtggtatat cccccttctg aaaaagaagc cccaggaagc attgttcgct
     5281 ggcaaagaga gcgagtcgtt gaagcgcaat gcgaacgccg ttgcgatgat ttcaaaggct
     5341 atatgcggta tccgggcagg ttgttttccg cctttgccgc atcaatccct gaatctggac
     5401 tatttcacac taccacaggc ggcacgctac gaggcgtggc gcgtggcaga aaaaacagat
     5461 cccttcaaag aaaatccgac gcccgtccac tgaacaaaag atgcgccctt cggacgcatg
     5521 cttgatccgg atccagacta aatatccgcc agccgttttc ttgcttcgac cagggcgaca
     5581 cgtacctgtg caggggctgt ggccccgggc tggcagcgcc gtgccagaat agtttctatt
     5641 tgcagggcat caaaaacatc ttcctcgact tcgggacaaa agcgtcgaaa ctcgtccaga
     5701 ctcaacgccg tcagcgtttt cccgacacca atgcaatagc gcactgtttc tcccacaata
     5761 tggtgtgcct cccggaacgg aacaccttta tagaccaggt aatcagcaag atcagttgct
     5821 tccatgaacc cgtcgcctac agctctttcc atcacgtcgc ggcgcaccgt aatcgtccgg
     5881 agcatggcag tgaaaacaga aaggcataac tggacggtat cagaagcatg gaaaaccggc
     5941 tctttgtctt cctgcagatc ccggttataa gtcagaggca gccccttcag cagcaccagc
     6001 agcgcctgat gatctgcaaa aacacgcgcc gccttccctc tggtcagctc tgcggcgtcg
     6061 ggattttttt tctgcggcat gatactggag cctgttgtaa acgcatcgcc cagcgaaatg
     6121 aaactgaagg gcgggctgtt ccacagaata atctcttcac tccaacggga caggtgcatc
     6181 atgatcaggg atgcgcagcc gcagaactca atcaggtaat cccggtcgga cacggcatcc
     6241 atgctgttgg cggacagttt ttcaaaaccg agcagttcgg ctgtgtaggc gcggtcaatg
     6301 ggatggggtg ttcccgccag tgcggcggaa ccgaggggca tcacattgat tctttttctt
     6361 aattgggcaa aacgttcccg gtctcttgaa aacatttcaa aataggcaag catatgatgt
     6421 gccagcagga ctggctgtgc gggctgtaaa tgggtgtagc ccgggagcac aacctgaacg
     6481 tgctcttcgg caaaatggag aatggcatgc tgcagcgcat gaagggcggc atcaatggca
     6541 tcagcctgat ctcttgtcca aagacgcatg tcggtagcaa tctgatcgtt tcgactccgg
     6601 gcagtatgca gttttttccc ggcatctccg acttttttta taagaagggc ttctatattg
     6661 ccgtggacgt cctccagccg ggaatcccat tgaacagaac cgttccggac ttcttccgcc
     6721 agttcggcaa gcccctggtc caggagactt ccttcttcct ttgtaataat tccgcacgct
     6781 gccagcattt tggcatgggc acggctcgcc tctatatccc agggcgcaag tcgatgatca
     6841 aaagacacgg actctcccag tttttccatc agcgaatgcg tcgtcccgtc aaagcgaccg
     6901 ccccataatt tgctcataaa aatacctcgg gctgcttgca aaaaagcatt cttttagaat
     6961 taacttcctt cgtgcgcctg ttcctgaaaa aggacaggcg tgccgaaggc atcactattt
     7021 tttcttttta acgggcgctg aacttttctg aatcagtgtg tccagctctt tcaatatcgt
     7081 ggcaaggatt tcttttctgt ttgcttccgt gattttgtaa tgctgtgcat tttccaaaag
     7141 ttccatggtg cgcaaactca cctgatacgc aaaatcctgt actttcatga tcgtacctcc
     7201 tgcagcccaa acaaattaat gcacatcagc ctatatgata caaaacgggc tgcgtcccgc
     7261 ccaagcggtg cgcaacccgt atttttacaa tggtaattca ggctgaaaag tcagcgcctt
     7321 tagacctatt taaacagcgc tgtaatggct gccggatcca tgcgcatgcc gggacccatg
     7381 gtgctgctca tggcgagccc tttcagatag gtacccttgc aggcactcgg gcgggccttc
     7441 ataatggctt cgattacact cttcgcattt tcttccaaca tctgggcatc aaaagatact
     7501 ttcccgatag gcacctggat attggcgaac ttgtcaacac ggtactcaat tttaccggct
     7561 ttaatctctt ctacagcctt gccgacatct gccgtcaccg atcctgcctt gggactgggc
     7621 ataagtccgc ggggacccag gattttaccg aggcgaccga catgacgcat catggcaggc
     7681 gtggcaacag cggcgtcaaa atcagtccat ccggcctgaa ttttgttgac caggtcttcg
     7741 ccgcccactt cttcagcacc tgccgctttc gcagcattca tctgatcttc ctgatcgcag
     7801 aaaacaacaa cacgaaccgt tttaccggta ccgtgaggca gggaaacgga accgcgaacc
     7861 atttgatccg catggcgggg atcaacaccg agaaggaagg caagttctac tgtttcgtca
     7921 aacttggcgt tggcattttc ttttgccaga gcgacggact cggagagagt gtagaaatgg
     7981 aatttgtcaa ctttagcgcg atttgcctta acacgcttag aagaatgaga catgagatgt
     8041 tctcctgtgg tgcaaacgga acagttgttc ctcccacgtt aaaaggaata tgagaaaaag
     8101 gcttcgaagt cagccttcaa cacgcaaacc catactgcgc gctgtaccgg cgatcatttt
     8161 ttttgcggtg tcaatgtttg cggcattgag atcagccatt ttaagctctg cgatttcctg
     8221 gatctgcgca tcagttacag aacccacttt ggttttgttg ggctcggcag aagcctttgc
     8281 gagcttagcg gagcgtttga gcagaacggc ggcaggcggt gttttggtga taaaggaaaa
     8341 agagcggtct tcataaatag taataatgac aggaacaacc aaccccatct gctccttggt
     8401 tcgctcgttg aactggttgc agaaatccat aatattcaag ccatgctgac ccagggcagg
     8461 accgacgggc ggtgcagggt ttgcctgacc ggctttcacc tgcaacttga cttgggcttt
     8521 aattttcttt ttcttaggtg ccatggttca gggaactcca ttcagatttg ttcaatttgc
     8581 cagggctcta cctcgacggt agtctggcgg tcaaaaattt caacactaac tttgaggata
     8641 ttgcggtccg aatcaatttc ttcgatattg ccgaaaaagt tggcaaaagg tccctcattg
     8701 atacggattc gctccccttt atcaaaagag atcttcactt taggccgttc ccgctcaccg
     8761 cgaacgactt caagaatttg attcacttct tcatcttcaa gcggaacagg cacatttttt
     8821 gatccaatga acccgagaat gcttggtgtg tcacggataa ttgcccagag ctcaggatag
     8881 gtttccggat gttcgggcaa ttgcagcaga acatatccgg gaaagcattt ttgttttgaa
     8941 acacgctttt gcccgccgcg aacaacttca acctgctcga tgggaacaaa agcgttacaa
     9001 atcagatgct caagattttt ctgctgcgct tgtgcaagca gcatatcccg gacagaagtc
     9061 tctctgccgg aataggcatg cagtacgtac caccgcctgt tcagtccgtc ttcaggtggc
     9121 gtgttcaagg aaaaaccctt ttccacttct tcttctttcg ggctgagttc gaattcttcg
     9181 tacggagtac tattttgtgt tgtgtctgcc acgagaaccc ccgttaagcg gccagtttca
     9241 gaataagcat aatgaaaatg ctgaaaaccc gatcatacac aaaaacaaca ccggccatga
     9301 tagcaataat aaaaagcgta accttggttg atgccatcag gtcttcccgg gtcggccacg
     9361 tgactttttt cagttcagcg atgacactgt caaagaagtc ctgaagcctg gcgaagatac
     9421 ccggctcttt tacgggagca cttacctgct ttgccatgaa ataaaattcc tgcactggca
     9481 cacgccagtc tttactcttc tgatacaata ccagatgtta aggtggcagg ggtggaggga
     9541 ttcgaacccc caaccttcgg ctttggaggc cgctgcacta gccattgtgc tacacccctt
     9601 caagcatgcc ggaaggctcc attccgggga tgacaggaac cttccggaga tttaacttgt
     9661 aaaaaaccga tctgcaacgc agagcggccg gatcagacta ctggataatt ttagtgacca
     9721 caccggcgcc gaccgtacga ccgcctt
//
LOCUS       DAOPWB010000117         9524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7229_length_9524_cov_4.572183, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000117 DAOPWB010000000
VERSION     DAOPWB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7229_length_9524_cov_4.572183"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKN92_10145"
     CDS             complement(<1..344)
                     /locus_tag="PKN92_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HNZ49195.1"
                     /translation="MNAENKKNTLPKPLTRGQKIQLAVLPVLLADIFKLLYKSCSMEV
                     RGKEHVEAALAPQGRCHMAIWHEAMAVAGCFYGGHDYTTMTSQSYDGEFAARVIRRWR
                     IGAARGSSSRGG"
     gene            complement(341..1219)
                     /locus_tag="PKN92_10150"
     CDS             complement(341..1219)
                     /locus_tag="PKN92_10150"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNZ49196.1"
                     /translation="MSQVAIIGPGALGCLFAVRLMQQGTGVTLVDYREDRCRLLMRQG
                     IMLETGSRTKTVFPAVSLQVPGEISLVLVLTKACALKTLSLPVAVPTLTLQNGLGITA
                     RLAEKMNEDWLLAGTTTEAATLLAPGKVRHTAPGLTCIGSAAAYSAEPVVALLRDAGF
                     NCVQVKNPEEIRWRKAVLSAAINPLTALLQVPNGELVKHDESRKLLRMLAEEALAVAQ
                     AYGYCQDFDAASEAESLCLKTADNLSSMLQDLRRGSPTEIDVLSGALLAYGDLRNMTL
                     PTTALIYRLIRSLEQK"
     gene            complement(1247..2233)
                     /locus_tag="PKN92_10155"
     CDS             complement(1247..2233)
                     /locus_tag="PKN92_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015356843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNZ49197.1"
                     /translation="MKDIKIPKLSIPLVKRANGKRLTAQFPAGFIILVLIAVFSKGCF
                     LDFKSSDKESEKKETAAAPVPVEKETYAIPLSQVQNPPASVPAPDQAQNPVNTPATTE
                     VPPAVAEAPAPSAAEQPVATEVAQRTAETMTPRSPFSDNKPILTTLPPLPVPRRNEMQ
                     VAAPSRQVPPETQPAAASAAAAPAPAPAPAAAPAPAPAAAAAAVPELTPVDPVEPLIT
                     VAQPAPQPAPAPKAAPAPQKEAAPAKAPSGRGKFGVQLGAFSGAERQAKAQALQRKVK
                     EKAGMTAEIDISSNKEIYKVIVVGFPDKEAATKALPGLKQKLGISEGFVKAL"
     gene            complement(2238..2861)
                     /locus_tag="PKN92_10160"
     CDS             complement(2238..2861)
                     /locus_tag="PKN92_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49198.1"
                     /translation="MEIKINRCSDACHQCEAVFAHQDKIHSVAQWNDSKLERQDFCKH
                     CFADREEGDVWCAWASQYVDPKVAEAERQESFSPLRRLFYDLAASEERSSLAQAFLAA
                     QLLKRQRVFRQIRETEEEQEQGTCRVCLYLDRAGNRLIETRDLNFSYTELDNARVLLM
                     EELRQRETPSEENAQEAIAPLETKEESPTEAVTEGTPSGAGPADMQE"
     gene            complement(3074..4810)
                     /locus_tag="PKN92_10165"
     CDS             complement(3074..4810)
                     /locus_tag="PKN92_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNZ49199.1"
                     /translation="MSGCFKLTLKAGAVFLFLLAVFLYFYCVLPFWGMPFNAQRRGNL
                     PLTPAWALEPWIWEDDTVTAASTMELLDGYLEHDFPVGTILIDSPWSLRYNDFTVDEE
                     RFPDPENFFKTIKSRGIRICLWMTPNVNSRSDDTGVPESQDWYQEAADRGYLVGGGTQ
                     YKWWKGRGGLIDYTNPEAMKWWRGLQQQVLDWGIDAWKLDDTASYFSRPVFGKVPIFY
                     QKTHQGWMTTRGYMDHYYRDEYQHGLTKNPEFVTMSRAIDSVLPWVHPEGFAPQDASP
                     LNWVGDNTHTWRDADRGLERALRCILRSAELGYNLPGSDIAGYHGDKIIPPEIYIRWT
                     QFSTFCGFFLNGGHGERRMWMRSDEEFSLVREYSWLRSELVPYIYSHVVETHKGAPVL
                     MRIVKGKYQYLFGESLLVAPIYEESAERTVLLPPGKWRYWFDDTKLIDGGTTLQRNYP
                     MAQYPVFIKEGAIIPMNISRGYTGIGEEDWAGMLTLNIYPAASSAFTVHHPDKSGALQ
                     VKVQKDAGAVTVDLEGKAKAHILRILLDQKPQILRFQGKELPEDTWTWDAEKQRLVIR
                     NEEEQTGRYEIR"
     gene            complement(4951..6270)
                     /locus_tag="PKN92_10170"
     CDS             complement(4951..6270)
                     /locus_tag="PKN92_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNZ49200.1"
                     /translation="MTLAVALLASSLFSQTVWQPTWESIDSRPSPQWFEDAKFGIFIH
                     WGVYSVPAWGPKDRYSEWYWHDMMDKKGETWQFHVKTYGEKFKYQDFAPLFKAEMYDP
                     QHWAETFRRSGAKYVVLTSKHHEGFSMWPDPTNWNWNVMDVGPHRDLAGDLVTAVRQE
                     GLRAGFYYSFYEWFNPLYKSDVDRYVDQYMLPQLKDLVERYQPDLVWPDGEWEHPSST
                     WRSTEFLAWLLNESAAPKDVAINDRWGKDCRSIHGGFATPEYQHRPKGPLMENQRFEE
                     CQGMGKSFGYNRNETAENYRSATELIHLLIDTVSRGGNLLLDVGPRADGTLPEIMEER
                     LLDMGKWLERNGEAIYGTVPWQNGSQDKNLRFTQKGQTKYILCLQWPGREIVLPDVAQ
                     LAGCKAQLLGSDAAVSLAQKDNDLKIAIPKVSVDAGLSHAFVIRLEP"
     gene            complement(6392..7843)
                     /locus_tag="PKN92_10175"
     CDS             complement(6392..7843)
                     /locus_tag="PKN92_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNZ49201.1"
                     /translation="MNTTDSVKKKQSTESDRAERLGKDSIPRLLIRFSVPAIIGMLAQ
                     ALYNITDRIFIGMAIGTDGIAAATIAFPFMMLTLACGMLIGFGGTSLISIRLGEGRQH
                     QAEHILGNMLVMLVLLSSAVTVFGLRFLDPLLVAFGAGESIMPLARDYLQVILYGVIL
                     QMVGFGMNAAIRGEGNPRIAMFSMLISVIINAILTPLFLFAFGWGIRGAAWATIIGQG
                     TSALWVMYYFLGGKSLLKLKRFDLALNLRVSGQILAVGMPPFLMQVLAGLLQSIFNHQ
                     LRIHGGDIAIAAMGIIYSTLMMFLMPIFGLNHGVQPIIGYNYGARNPQRVSKALKTAM
                     FLASLCTCCGFLVIMLLPRQIIFLFNSKDAELLAVGVRAMRLTLCMLPIIGFQVVSSG
                     YFQAVGRPRTSAVLMLSRQLFFLFPAVIILPRFMGITGIWLSIPFSDGVAALLAGTMI
                     SLELRRLHQSIQREDNADALPDAFDEEGLAESS"
     gene            8150..8572
                     /locus_tag="PKN92_10180"
     CDS             8150..8572
                     /locus_tag="PKN92_10180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNZ49202.1"
                     /translation="MSFSRRSFVGFMAAAGFAGNARFAAAEGSCGAKCSAAKAVYVVA
                     TVTVKAGKREEFVRIFKENVPNVLAEEGCIFYDPLVDVDSGISAQGALRPEVMVVAEK
                     WASLEHLKAHLAAPHMAAYREKVKDLVISTELQIMHQA"
     gene            8620..9144
                     /locus_tag="PKN92_10185"
     CDS             8620..9144
                     /locus_tag="PKN92_10185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNZ49203.1"
                     /translation="MKSGSSIWMTGVIPVLAIAMLLVFSRGLRGATNPLEGKEAPAFS
                     LETLAGGTFDLKEHLGKRPVVLDFWAVWCPPCRASLPKVAETVKGLGDADLAFSTVNL
                     GDSPDKITEFLKSKNLEDLPVALDKNREAAKLYGVQSIPTMVLIDKEGKVAVVSVGAM
                     TESALSAALKKLLD"
BASE COUNT         2206 a         2634 c         2526 g         2158 t
ORIGIN      
        1 ctcccgcctc tggacgaaga tccccgggca gcaccgatac gccaccgcct gatgacccgg
       61 gcggcaaatt caccatcata gctctggctt gtcatggttg tgtaatcatg cccgccgtaa
      121 aagcaacccg ccaccgccat ggcttcgtgc cagatcgcca tatggcacct gccttgcggg
      181 gcaagcgccg cttccacatg ttcttttccg cgcacttcca tggaacaact tttatacagc
      241 agtttgaaaa tgtccgccag aagaacaggc agtaccgcca gctgtatttt ctgtccccgc
      301 gtcagaggct tgggtaatgt attttttttg ttttcggcgt tcatttctgc tccaggctcc
      361 gaatgagccg atagatcagc gcagttgtcg gaagggtcat gttccggagg tccccatagg
      421 ctaataaagc tccgctgagc acatctattt cagtcggact tccacgacgc agatcctgca
      481 gcatggaaga aagattgtcg gctgttttca ggcaaagact ttccgcttct gatgcggcat
      541 caaagtcctg acaatacccg taggcctgag cgaccgccag tgcctcttcc gcgagcatac
      601 ggagcagttt ccggctttca tcatgtttca cgagttcacc gttgggcacc tgaagcagcg
      661 ccgtaagagg attgatggca gcactgagca cggccttgcg ccagcgtatc tcttcaggat
      721 tcttcacctg cacgcaatta aagcctgcgt cccgcagcaa ggcaacaacc ggttctgcag
      781 aataagcggc cgccgaaccg atacaagtga gcccaggtgc cgtatggcgc accttgccgg
      841 gtgccagcag tgttgccgct tcagtggttg tcccggcaag cagccagtct tcattcattt
      901 tttcggcgag ccgggcggta atgcccagcc cgttctgcag tgtcagcgta ggcacggcga
      961 caggaagact caaggttttc agcgcacagg ctttcgtgag taccagcacc agagaaatct
     1021 ccccgggcac ctgaagcgac acagcgggaa aaaccgtctt cgtcctgctc cctgtttcca
     1081 gcataattcc ctgacgcatt aaaaggcggc accggtcttc cctgtaatcc accagagtca
     1141 ctccggtacc ctgctgcatc agacggacgg caaaaagaca gcccaatgct ccgggcccga
     1201 taattgccac ctgactcatg cagtttcctt cacgaagcga gttaaattac aacgccttta
     1261 caaagccttc ggagattccc agcttttgct tcagcccggg aagggctttg gtggcggcct
     1321 ctttatcagg aaatccgacc acgatcactt tgtagatttc tttgttggaa gaaatatcga
     1381 tttccgccgt cataccggct ttttctttca ccttacgctg caatgcctga gcctttgcct
     1441 gtctttccgc accgctgaaa gcgccaagct gcacaccgaa ttttccacgg ccggaggggg
     1501 ccttcgcagg tgccgcttct ttctgaggcg ctggcgccgc tttgggagca ggggcgggct
     1561 gcggagcagg ttgtgcaacg gtaatgagcg gctccaccgg atccacgggt gttaattcag
     1621 gcacggcggc cgcggcagca gccggagccg gggccggggc agcagccgga gccggggccg
     1681 gggcaggagc tgcagctgcg gaggcagcag cgggctgtgt ttcggggggc acctgccggg
     1741 aaggcgccgc cacctgcatc tcattgcgcc gtggtacggg caaaggcggg agggtggtca
     1801 gaatcggctt attgtcggaa aaaggacttc tgggcgtcat tgtttccgcc gtacgctgcg
     1861 ctacttcggt ggcaaccggc tgctccgcag ctgaaggcgc cggggcttct gccacagcgg
     1921 gcggcacttc tgtcgtcgca ggtgtattta ctggattctg tgcctgatca ggcgccggta
     1981 cagatgcagg cggattctgc acttgcgata aggggatcgc ataggtctct ttttccacgg
     2041 gaacaggcgc tgccgccgtc tcttttttct cagattcttt atccgaagac ttgaagtcca
     2101 ggaagcagcc tttgctgaac acagcaatca gaaccagaat aataaatccg gcagggaact
     2161 gtgccgtcag ccttttcccg tttgcacgtt tcacaagagg gatggagagt tttggaattt
     2221 taatgtcttt cattgcctta ctcctgcata tcagccgggc ctgcgccgga cggcgtccct
     2281 tccgttacag cttctgtggg agattcttcc ttcgtttcca gcggggcgat agcttcctga
     2341 gcattttctt cggacggtgt ttcccgctgc cgcaactcct ccatcagcag cacacgcgca
     2401 ttatccagtt cggtataact gaaattcaga tctcgtgttt cgatcaggcg atttcccgcc
     2461 cggtccagat acaggcagac acgacatgtc ccctgctcct gctcctcttc cgtttcgcga
     2521 atctgccgga agacacgctg ccttttcagc agctgcgccg caagaaaggc ctgtgcgaga
     2581 ctgcttctct cttccgaagc ggcgagatca taaaaaagcc tgcgcagcgg ggaaaatgac
     2641 tcctggcgtt ccgcttccgc cacttttggg tctacatact ggctcgccca ggcacaccag
     2701 acatcacctt cttctctatc ggcaaagcag tgtttgcaaa agtcctgccg ttccagtttg
     2761 gagtcattcc actgtgcaac agaatgaatt ttatcctggt gggcaaaaac agcttcacat
     2821 tggtgacatg catcactgca tctgttaatt tttatttcca tcacaggtta ccggaggtta
     2881 ttgtccatgc cgtctgctta ctatacatag tggttttcgc cactgaaaca ggcggattca
     2941 ttacaacata tagtatactc gatttctttc gcaggagcaa agaggggaaa gagcattcgg
     3001 aagcacagcc ccgcaccgaa gcggcgggca gaaaagcggc ttcataagac atatcgggcg
     3061 cagactggag cactcagcga atttcgtatc ggcctgtttg ttcctcttcg ttgcgaatga
     3121 cgaggcgttg cttttccgca tcccacgtcc aggtatcttc aggcagttct ttcccctgaa
     3181 aacgcaggat ttgaggcttc tgatccagaa gaatccggag gatatgcgct tttgcctttc
     3241 cttccagatc aactgtcacg gcaccggcat ctttctgcac ttttacctgc agtgccccgc
     3301 ttttatcggg gtggtgcacg gtaaacgccg aagaggcggc ggggtagatg ttcagcgtca
     3361 gcatgcccgc ccagtcttct tcaccgatac ccgtataccc ccgggaaata ttcatgggga
     3421 taatggcacc ttctttgata aaaacagggt attgcgccat gggatagttg cgctgaagtg
     3481 tcgttccccc gtcaatgagt tttgtatcat cgaaccagta acgccatttt ccgggcggca
     3541 gcagcaccgt ccgctccgct gactcttcat aaatgggcgc aaccagcaga ctttcgccga
     3601 agagatactg gtatttccct tttacgatac gcatcagcac gggcgcaccc ttatgggttt
     3661 ccaccacatg gctgtagata taaggtacga gctcgctcct gagccaggag tactcccgta
     3721 ccagcgaaaa ttcttcgtcg ctccgcatcc acatgcgccg ctcaccgtga ccgccattca
     3781 gaaaaaaacc gcagaacgtg gagaactgcg tccagcgaat atagatctcg ggcgggatga
     3841 tcttatcgcc gtgatagcct gcaatatcag atccgggcag gttataaccc agctcggcac
     3901 tgcgcaggat acaccggagt gcccgttcga gtccgcggtc ggcatcacgc caggtatgcg
     3961 tattgtcgcc gacccagttg agaggcgagg catcctgtgg tgcgaagcct tccgggtgaa
     4021 cccagggcaa cacactgtca atggcacggc tcatggtcac aaattcggga ttcttcgtca
     4081 ggccgtgctg atactcgtcc cgataataat gatccatgta ccctctggtc gtcatccatc
     4141 cctgatgggt tttctggtag aaaatgggta ctttcccgaa gaccggcctg gagaaatagc
     4201 tggcggtatc gtccagtttc caggcatcaa ttccccagtc cagcacctgc tgctgcaaac
     4261 cgcgccacca tttcatggct tccggattgg tgtagtcaat aagtccgccg cgccctttcc
     4321 accatttata ctgggtgccg ccgccgacca gatatcccct gtccgccgcc tcctgatacc
     4381 agtcctgtga ctccggcact cccgtatcgt cactgcggct gttcacgttg ggtgtcatcc
     4441 acaggcagat gcggatgccc cgggatttga tcgttttaaa aaaattctca ggatcaggga
     4501 aacgctcctc atccaccgta aaatcgttat accgcagcga ccaggggctg tcgataagaa
     4561 tggtgcccac cgggaaatca tgctccagat agccgtcgag gagttccatg gttgatgccg
     4621 ccgtgaccgt atcgtcctcc cagatccagg gttccagagc ccaggcaggg gtcagcggga
     4681 gatttccccg gcgctgtgca ttaaacggca tgccccagaa gggcagcacg cagtaaaagt
     4741 agagaaagac ggccaggaga aacagaaaaa cagcccccgc tttcagtgtg agtttaaagc
     4801 agcctgacat ggaaactcct tttccgcttt agggcggtct gttgaggaag aagaaaaacg
     4861 gcagcacgga ataaaatacc gcttcctccc ggagaaagcg gtatgagacg catcataaat
     4921 cttgtgtact taaaaaaaca atagcgccgg tcagggctcc agtctgataa caaaggcatg
     4981 actcagaccg gcatcgacag ataccttcgg gatggctatt ttcagatcat tgtccttttg
     5041 cgcaagactc actgctgcat cactgcccag cagctgcgcc ttgcagcctg caagctgcgc
     5101 cacatcggga agaacgattt cacggcccgg ccattggagg cacaaaatgt attttgtctg
     5161 tcccttctgt gtgaagcgca ggttcttatc ctgagacccg ttctgccacg gcacggttcc
     5221 ataaatcgct tcaccgtttc gctccagcca tttacccatg tccaggagcc tttcttccat
     5281 aatttccggc agtgtgccgt cagcgcgggg tcccacatcc agaagcagat taccgccccg
     5341 gctcaccgtg tctatgagca gatggatcag ttctgtggca ctgcggtaat tttccgctgt
     5401 ttcgttgcgg ttgtagccga aagatttccc catgccctga cattcttcaa aacgctgatt
     5461 ttccatcagc ggtcctttgg gacggtgctg atattcgggc gtggcgaagc cgccgtgaat
     5521 gctgcggcag tcttttcccc agcggtcatt gatcgccaca tctttcggtg ccgcggattc
     5581 attgaggagc catgcaagaa attccgtgct gcgccaggta ctgctcggat gttcccattc
     5641 cccatcgggc cagaccagat cgggctgata acgctcaacc agatctttca gctgcggcag
     5701 catatactga tccacatagc gatccacatc acttttgtac agcggattga accattcata
     5761 gaaggaatag tagaagcctg cccgcagtcc ttcctggcgc actgccgtga cgaggtcacc
     5821 ggcaagatca cgatgcggtc ccacgtccat cacattccag ttccaattgg taggatcggg
     5881 ccacatactg aagccttcgt ggtgcttgga agtgagcacc acatattttg ctccggaacg
     5941 gcggaaggtt tccgcccagt gctgcgggtc atacatttcc gctttgaaca ggggcgcgaa
     6001 atcctgatac ttgaacttct cgccataagt cttcacgtga aactgccagg tctccccttt
     6061 cttgtccatc atgtcatgcc agtaccattc tgaatagcgg tctttaggac cccaggcagg
     6121 cactgaataa acaccccagt gaatgaaaat accgaacttc gcatcctcaa accattgcgg
     6181 gctggggcgg ctgtcaatgc tctcccaggt gggctgccac acggtctgcg aaaaaagaga
     6241 agaagcaagc aatgctactg caagggtcat gatcaaatct ccttaataaa gggctttccg
     6301 ccttactcaa gggtctggct gcaggacaca gaccctccgg atctcatgct ttttatcata
     6361 gcataccgca ggaaaaaagg cggaaaattg attaagaaga ttcagcaagt ccctcttcgt
     6421 caaaggcatc ggggagcgca tccgcattgt cttcccgctg aatgctttga tgcaggcggc
     6481 gcagttccag cgaaatcatg gtgcctgcga gcagggcggc gacgccgtcc gaaaagggaa
     6541 tggaaagcca gatgccggta atccccataa aacggggcag gataatcacg gcgggaaaaa
     6601 gaaaaaagag ttgccgcgac agcatcagca cggctgaagt gcggggcctt ccgacagcct
     6661 gaaaataacc ggagctgacc acctgaaaac cgataatagg cagcatgcac agggtgagcc
     6721 gcatggcccg gaccccgaca gcaagcagtt ccgcatcttt gctgttgaaa agaaagatga
     6781 tctggcgtgg cagaagcatg atgacgagaa agccacagca cgtgcagagt gaggcgagaa
     6841 acatggcagt tttcagcgct ttgctcacgc gctgcggatt cctggccccg tagttatacc
     6901 cgataatagg ctgcacacca tggttcagcc cgaagatggg catgagaaac atcatgagcg
     6961 tggaatagat aatgcccatg gcggcaatgg cgatatcccc gccgtggatg cgcagctgat
     7021 ggttgaaaat gctctgaaga agccctgcca gcacctgcat cagaaaaggg ggcatgccca
     7081 ctgccagtat ctgcccggaa acccgcagat tcagggcaag atcgaagcgt tttaacttca
     7141 gcaggctttt tccgccgaga aaatagtaca tcacccagag ggcggaggtg ccctgtccga
     7201 tgatggtcgc ccaggcggca ccgcggatcc cccagccgaa agcaaagaga aaaagcggcg
     7261 tcaggatagc gttgataata acgcttatga gcatggaaaa catggcgata cgcgggttgc
     7321 cctcgccacg gatggctgcg ttcatgccga agccgaccat ctgcagaatc acaccataga
     7381 ggataacctg cagataatcc cgtgccagag gcataatgct ttcgccagcg ccgaaagcaa
     7441 ccagcagggg atcaagaaat cgcagcccga atacggtcac ggcggaagac agcagcacca
     7501 gcatgacaag catattgccg aggatgtgct ccgcctgatg ctggcgaccc tcgcctaagc
     7561 gaatagaaat aagggatgtg ccgccgaagc cgataagcat tccacatgcc agcgtcagca
     7621 tcataaaagg aaaggcgatg gtggcagcgg cgatgccatc ggtaccgatg gccataccga
     7681 taaaaatgcg gtcagtaata ttgtacagtg cctgggcaag catgccgata atggcgggca
     7741 cagaaaaacg gatcagcagg cgcggaatgc tgtctttgcc caaccgctcc gcccggtcgc
     7801 tttcggtgct ttgttttttt ttgactgaat ctgttgtatt cataaattcc aattctgcgt
     7861 tgcaacggca tgaaacagtt cttcagtcat cggatacggc tgacagaaac acttcaccct
     7921 tcactttaaa aagtgtaaca accaaaagcg ggtaattaaa tcccgcaggg cagttttttg
     7981 agaaaaactg cagaaaggag gcagctgaaa acggatcagg aagccttctc ttcccggtgg
     8041 cgcaccgaaa tattttacat ggacaggttg tttcacctta aaattggtat acttgtgttt
     8101 gttgtggaca ctgaaaatgc atagaagcat ctgtgcgggg agagtcatca tgtctttttc
     8161 acgaagatct tttgtaggat tcatggctgc agcagggttt gccggcaatg cacggtttgc
     8221 tgcggcagaa ggcagctgcg gggcgaaatg cagtgccgcc aaagcggtgt atgtagtggc
     8281 aacggttact gtcaaagccg ggaaacgtga ggaatttgtc cgtattttca aggaaaacgt
     8341 tccgaacgtg cttgcggaag agggttgtat tttttatgat cctctggtcg atgtggactc
     8401 aggcatcagc gctcagggag cgctgcggcc cgaagtcatg gtcgtagcgg aaaaatgggc
     8461 aagtctggaa catttgaaag cgcatctggc ggcgccgcat atggcggctt atcgtgaaaa
     8521 ggtcaaagat ctggttatca gcactgaact gcagattatg catcaggctt aaccggcgga
     8581 tattgcatca ttcccggacg ggaaagaaag gaaatcagta tgaaaagtgg cagctctatc
     8641 tggatgaccg gagtcatccc cgtactggca attgcaatgc ttctggtgtt ttcccggggc
     8701 ctgcgcggcg caaccaaccc gttggaagga aaagaagcgc cggctttttc actggaaaca
     8761 ctggcaggag gtacgtttga cctgaaagag catctcggca aacgccctgt cgtattggat
     8821 ttctgggcgg tatggtgccc gccctgccgg gcgagcctgc cgaaggttgc ggaaacagtg
     8881 aaaggactgg gtgatgcaga tctggcgttc agtaccgtca acctgggcga cagtccggat
     8941 aaaatcacag aatttctgaa aagcaaaaac ctggaagatc tgcctgttgc tttagacaaa
     9001 aaccgggagg cagccaaact ctacggcgtc cagagcattc ccaccatggt gctcattgat
     9061 aaagaaggaa aagtggcagt tgtgtctgta ggcgccatga ctgaatccgc tctttccgcc
     9121 gccttgaaaa aactgctcga ctgagttttg attttttgca tgcccgaaaa cagcactgct
     9181 tcacctttaa atgcagggac agactacgga ataaattatt ttttgctttt taagtagtct
     9241 gtccctgcat tttcccgcct caggcatcat tgctgctttc tcctccgtca ttgctgcctc
     9301 tcctccctcg tcattcctgt gcgcctcccc actgggaacg ccaatctccg cattggcaaa
     9361 caagcgggct aagacgaaga caggcgcacg gctctgagtc ggagtaatgt ctcgggacat
     9421 acacacaaca tcacagacca cacacgctcc tgccatttct cccttccgac gttacgtcca
     9481 ccctcaggcg tcatggctgc ctcccccggc gtcattcctg cgca
//
LOCUS       DAOPWB010000118         9431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7314_length_9431_cov_5.272184, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000118 DAOPWB010000000
VERSION     DAOPWB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7314_length_9431_cov_5.272184"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..928)
                     /locus_tag="PKN92_10190"
     CDS             complement(38..928)
                     /locus_tag="PKN92_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ49204.1"
                     /translation="MKKIHMKRRDFLKSSAAAALLLGAAGRGRAEETAFSTELKKALQ
                     YAMLPKELSDADKFKLALDCGFEGIEVDRIESVDEAKKLGELAREIGTPIHSLVFGGW
                     DMPFSDPNPKVIQRALDAMSTQLRVANALQADTILLVPVVVNKKATTEQAWERSRKHI
                     PELIPLAEEMNIVIALEEVWNGFLLDSASEFARYVDEFNSPWIQAYFDVGNVLKYGDP
                     VEWIRTLGKRIRKIHLKDFRKAGEKWTNLRDGDVDWPAVRKAIDEVGYNGYLTTELGG
                     GDAAYLKDLGERCDLIIAGK"
     gene            complement(1079..3085)
                     /locus_tag="PKN92_10195"
     CDS             complement(1079..3085)
                     /locus_tag="PKN92_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49205.1"
                     /translation="MTAACNARCVRLASFFLIALSLSIGWGIRGNFGHEAGAMIAGVL
                     SGIAVCLLSGRKDWHPRVAFFAFFGGIGWAFGGSISYMYPISFLGSEQWATAAYGFFA
                     TFLVGFLWAGLGGMGTALPAVLERERLNRFLAPVAFVLLIMAISDLWIHPWLADHLVI
                     PGANLSDVTWNRHHNPLYWLDADWFAAVVALTAVCLYDLVNRRFQKGTRLLFFTGIGV
                     LLGGAGYALLEHFHLIQPLFGWLVVPLGDASALNPATGLPYGSDAFLSNWPNFVHYYP
                     HYMGPLLGACIGAALYFRRWGTWNNNSGFILYLAGGWLLAFLMLPVLGSIPLQSFGGL
                     RLTPPRSDDWAGIVGVFTGGSLYCLRRNLQSVAYTASLTGILGGFGFAFVPFARSFLR
                     LPGHPLLTPGGTPLFWEHYQSANWHSVMEQSHGFCHGLALAIAIAFLAKSTPKLEDPL
                     PKKRLPEGFSVAFVLLFITLMNVFKNVEEWTKESVQAVPLTMKAPFFASWNWSTQAWF
                     LLIWTLLSAVLILLMIWHTRKALPVVPASYLARGQLLYLTFLWIMVIANFERALVSFH
                     ESRLVTEAVIIFNAGLASLLILLLPQEEADVTVCDSNPFPGSLKRLWLRAIPLLICLL
                     LAMTAALRLAYRDQPVENPSTNHKRWGEEAQWRIRPILKGGRHL"
     gene            complement(3113..4465)
                     /locus_tag="PKN92_10200"
     CDS             complement(3113..4465)
                     /locus_tag="PKN92_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HNZ49206.1"
                     /translation="MVQGPVLVAVVLFSVLFIVAATAKFRLHPFLALIAAAYLTGILA
                     HMPLTELVDTVNTGFGNMMRHIGLVIITGTMIGVILEKSGAALCMAEKTLSLLRGRAP
                     QLAMSLIGGLVSIPVFCDSGYVILSSLNKAVARRARVPLASMSIALATGLYATHTLVP
                     PTPGPIAAAGNLGASDYLGLVILVGLVVSCFAIAAGYFWAMLVAARFAIEGEGKEEIT
                     GDIGPTQNLPPVFKSFAPIVIPVLLIGLASVVRFSSCQSTAAGFILFLGTPVTALLIG
                     FLFALLLLPRADGALLNEWVKEGVLHAAPILLITGAGGAFGAVLSATPISDLVKELAE
                     GGLLGGPLVLLLPFLIAAGLKTAQGSSTASLVMTSALVAPFLPTLGVTSPLQLSLMVM
                     AIGAGSMTVSHANDSYFWVITQFSGMSVKTAYKTLTLGTLIQGITSFLVTCLLYWIFV
                     "
     gene            complement(4500..5843)
                     /locus_tag="PKN92_10205"
     CDS             complement(4500..5843)
                     /locus_tag="PKN92_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49207.1"
                     /translation="MISSLLVLTAMLAAPAPWQIATFQTDITIPVGHACMGGGVKPAS
                     EIVDPLYALGVVLLGPEKPVVWVTVDWCEVRNDAYDHWRSALAEAAGTTPDRVLHTSV
                     HQHDAPVIDYAAQSLLDEAGLPGALCDSDFARACVERTAAALKEALKSPRPVTHYGIG
                     QAKVKQVTSNRRVVLDDGTVTYGRGSATRDPKLSEKDEGIIDPWLKTLSFWDGDRPLA
                     ALSAYSTHPMSYYGQGGVSADFVGMARAKRQAEMPDVFQMYFTGCSGDTTAGKFNDGA
                     PENRPVLAERMYEGMKAAWENTVCYPLEQVIFRKAPLYLEPKNFGGYSEEDALATLAN
                     ESAPTFHRNLAAMTLSWRKRVAEGRAIDVPAVDFGHARFLILPGESFVGYQLLAQGMS
                     PQAPVVVAGFGECAPGYIPTAAASAEDYNEDKWCWVEPGAEPLITDALALALDGR"
     gene            6024..7466
                     /locus_tag="PKN92_10210"
     CDS             6024..7466
                     /locus_tag="PKN92_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ49208.1"
                     /translation="MYESCGRRTFLTQTALSLGALPALLQVTAQTAESSRPNFLFLFV
                     DDYRADAIHALGCNAIRTPNIDRLVRQGTAFTNCYNQGGWHGAICMASRAMVMTGRNL
                     WDTHALDASLPEQAAAGKLWPQRMQASGYRTFMSGKWHVNCNPAKVFQRARHERPGMP
                     PDIESQYRRPEEQKPDPFDPADTTQGGYFTGGKHWSEVVADDGVAFLRKEAGDEPFFM
                     YLAFNAPHDPRQTAHAWLDQYTADALPLPPNFLPGYPYKDEIGCSADMRDERLAPFPR
                     TPFAIRTHLREYYAIITHLDAQIGRILDALERSGRAENTYIILAGDNGLSIGSHGFMG
                     KQNMYEHSMKVPLIMSGPGIAADRRCDALVYMQDFVPTLLELAAVAPETPMDFRSLLP
                     LLRGEESSLRPHIYGAYMMLQRMVRGERYKMIYYPTVSRYRLYDLQEDPFELQDLSEV
                     PEHGATLKELQEQLQRLQKEMHDPLSGSVS"
     gene            7486..9036
                     /locus_tag="PKN92_10215"
     CDS             7486..9036
                     /locus_tag="PKN92_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNZ49209.1"
                     /translation="MLTRRTFLKQTGAAAAMAALLPRLCHAQQMPNIIWICCDDHAQN
                     AISAYGGRLAEVAPTPNIDRIAREGALFRSSFVTNSICGPSRAVVLTGLFSHKNGYKD
                     NRSRFDGSQQTFPKLLQQAGYETALFGKWHLHSDPTGFHHWEILPGQGDYYNPDFITK
                     EGQKRNEGYVTDLITDKALHWLEHERDNGKPFMMMVQHNAPHREWLPSPKHLRTYEDT
                     VFPEPETLFDDYATRGTAAHKQDMTIEKTMRPGADLKLFRKEDEGSAEWKDIFERMTA
                     EQRADWEDAYRARNEEYWSGKYQGKELVRFKYQCYIRDYMRCVASVDDNVGRVLDYLE
                     TSGLDKNTLIFYSSDQSFYLGEHGWFDKRFMYEESLRTPLLARWRGVIKPGSEIDAMV
                     QNLDYAETFLAAAGAAVPANMQGTSMLPLLRGETADDWRKSIYYHYYEGEKAVHAVYK
                     HYGVRTERYKLMYFYTLDEWEFYDLQEDPHELRNEYNNPDYAEEIQKAKQELERLRQL
                     YEVPEDDE"
     gene            9049..9357
                     /locus_tag="PKN92_10220"
     CDS             9049..9357
                     /locus_tag="PKN92_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49210.1"
                     /translation="MKHPLGTFFHAAALILFLAGGGVLIYYEIYTLYLAWQHWSIWGI
                     LAAIILPGIFNIALFFYVCFSAGTLFTYYTFLSIIWLAPVIPAIILAAFAEGFKKKEG
                     "
BASE COUNT         2335 a         2552 c         2600 g         1944 t
ORIGIN      
        1 gcatcggcgg tcgttctatt ttgctttttt tcgaccgcta cttccctgca ataatcagat
       61 cgcagcgctc accgagatct ttgagatagg cagcatcgcc accacccagc tctgtggtca
      121 ggtagccgtt gtagccgact tcatcaatgg ctttgcgcac ggcgggccag tccacatcac
      181 cgtcccggag attcgtccat ttttcgcctg ctttacggaa atctttgaga tggatcttgc
      241 ggatgcgctt gccgagtgtg cgaatccatt caaccggatc accgtatttc agcacattgc
      301 cgacatcaaa ataggcctga atccaggggc tgttgaattc atccacatac cgggcaaatt
      361 cgcttgcact gtccagaaga aaaccgttcc agacttcttc cagagcaata acgatgttca
      421 tttcttcagc aagtggaata agttcaggga tatgcttgcg tgagcgctcc caggcctgct
      481 ctgtagtcgc ttttttgtta acgaccaccg gaaccagcag aatggtatcg gcctgcagcg
      541 cattggcgac acgcagctga gtgctcatgg catcgagggc acgctggatc actttgggat
      601 taggatcgga gaagggcata tcccagccgc cgaaaacgag ggagtgaatg ggggtgccga
      661 tttcccgggc aagctcgcca agtttcttcg cttcgtccac actttcaatg cggtccactt
      721 caatgccttc aaaaccgcag tcaagggcga gtttgaattt gtcggcatcg gacagctctt
      781 tcggcagcat ggcatactgg agtgcttttt tcagttctgt ggagaaagcg gtctcttcag
      841 cccggcctct tccggcagca cccagaagca atgcggcggc ggcactgctt ttcagaaagt
      901 cacggcgttt catgtgaatc tttttcatgt gtattttcct ttattctgta cagaccggtg
      961 ggtcaacatt attattcaaa ctgaacagtg taaaccattt tacgtgaagg ctgtgctctt
     1021 tcacaaatgg cgtggatgat tctccctgtt ctgcctgccg aagcagtcgt attagcgcct
     1081 acaagtggcg gcctcctttc aggatcggac ggatgcgcca ttgtgcttcc tcaccccagc
     1141 gcttgtggtt ggtggacgga ttttctaccg gttgatcccg gtaggcgagc cggagagcag
     1201 ccgtcatggc aagaagcagg caaatgagca gcggaatggc gcgcagccag agtcgcttga
     1261 gagaaccggg gaaaggattg gagtcgcaga ccgtaacgtc cgcttcttcc tgcgggagca
     1321 gcagaatcaa cagagaagcc agccctgcat tgaagataat cacggcttcc gtaaccagac
     1381 ggctttcatg aaaagagacc agcgcacgtt cgaagttggc aatgaccatg atccatagaa
     1441 aggtcagata cagcagctgc cctctggcaa gataagaagc aggaacaacc ggcagggctt
     1501 ttctggtatg ccagatcata agcagaatca gtacggcgga aagcagtgtc cagatgagca
     1561 ggaaccacgc ctgcgtcgac cagttccacg acgcaaaaaa aggtgctttc atcgttaaag
     1621 gcactgcctg aacgctttcc tttgtccact cttcaacgtt tttaaaaaca ttcatcagtg
     1681 tgatgaaaag cagaacaaaa gcaacggaga aaccctcagg cagccgtttt ttcggaagcg
     1741 gatcttccag ttttggcgtg cttttcgcca gaaaggcaat tgcaatcgcc agagcaagac
     1801 catggcagaa accgtgggac tgttccatca cgctatgcca gttggcactc tgataatgtt
     1861 cccagaagag gggtgttccc ccgggagtca ataaaggatg acccggcagc cgaagaaaag
     1921 agcgggcgaa gggaacaaag gcgaagccga agccgcccag aataccggtc agtgaagctg
     1981 tataagcgac gctctgcaga ttccgccgca gacagtagag gcttccgcct gtaaaaacgc
     2041 cgacaatgcc tgcccagtca tcgctgcggg gcggtgtgag gcggagcccg ccgaaagact
     2101 gcaaagggat ggagcccaga accggcagca tcagaaaagc aagcagccac ccgcccgcca
     2161 gataaagaat gaacccggag ttattgttcc atgtgcccca gcgccggaaa tacagagcgg
     2221 caccgatgca ggcgccgagc agggggccca tataatgggg atagtaatgg acaaaattag
     2281 gccagttgct tagaaacgcg tcactgccgt aaggaagacc tgtcgccggg ttgagggcgc
     2341 tggcatcgcc caggggaaca acaagccatc cgaagagcgg ttgaatcagg tgaaaatgct
     2401 ccagcagcgc atatcctgcg ccgcccagca aaacgccgat gcctgtgaag aacagcagcc
     2461 tggttccctt ctgaaaacgg cggttgacca gatcataaag gcaaacagca gtcagtgcaa
     2521 cgacggcggc aaaccaatcg gcgtccagcc agtacagtgg gttatgatgt ctgttccagg
     2581 tcacatcgct cagattggca ccgggaatta ccaggtgatc tgccagccac ggatggatcc
     2641 ataaatcgct gattgccatt atcagcagga caaaagccac cggagcaaga aagcggttta
     2701 accgttcccg ttccagaaca gcgggaagcg ccgtacccat cccaccgaga cctgcccata
     2761 gaaaacctac aagaaaagtg gcgaaaaagc cgtaggcggc agttgcccac tgctcgctgc
     2821 caagaaaaga gatgggatac atataggaga tggaaccgcc gaaagcccag ccgattccgc
     2881 cgaaaaaggc aaagaatgcc acacgcggat gccagtcttt tctgccggac agaagacata
     2941 cggcaatccc cgacagcacg cctgcgatca tggcacctgc ttcatggccg aaattgccgc
     3001 gaatgcccca gccgatggaa agggacaggg cgatcagaaa aaaagaagca agccggacac
     3061 atcgggcgtt gcaggcagct gtcatgtaag cggatccttt atagaggaca gatcagacga
     3121 atatccagta aagcaaacag gtaacaagaa aagaagtgat gccctgaatc agtgttccca
     3181 gggtcaatgt tttataggcg gtcttgacgg acatgccgct gaactgagtg atcacccaga
     3241 aataactgtc gttggcgtga gataccgtca tggaacccgc acctatcgcc atgaccatca
     3301 gactgagctg gagtggagag gtcacgccga gggtcgggag aaagggagcg accagtgccg
     3361 atgtcatgac gagggaggcg gtggacgatc cctgcgctgt tttcagaccg gcggcaatga
     3421 ggaaggggag cagcagtacc agcgggccgc cgaggagacc gccttctgcc agctctttga
     3481 ccaggtcgct gatgggagtt gcgctgagta ctgctccaaa ggcaccgcct gctccggtga
     3541 tcagaagtat gggggcagcg tggagcacgc cctctttgac ccactcgtta agcaaggctc
     3601 catcggcacg gggaaggagc agcaacgcaa aaagaaaacc gatgagcaat gctgtgacgg
     3661 gagttcccaa aaagaggata aatccggctg cggtgctctg gcaggaggaa aaacgcacga
     3721 cagatgccag cccgatcagc agcaccggga tcacaatggg agcgaaggat ttgaagacag
     3781 gcggaagatt ctgcgttggc ccgatgtcgc cggtgatttc ttcttttccc tcgccttcaa
     3841 tggcaaagcg ggctgccaca agcattgccc agaaataacc ggcggcaatg gcaaagcagg
     3901 aaacgaccaa tccgacgagg atcaccaatc ccaaataatc cgatgcgccc aaattgcctg
     3961 ccgccgcaat gggtccgggt gtgggcggca caagcgtatg cgtggcataa aggcctgttg
     4021 ccagtgcgat cgacatggat gccagcggta cacgggcacg gcgggcaacc gctttattca
     4081 ggcttgaaag aattacataa cccgagtcgc agaaaacagg aatgctgacc agaccgccga
     4141 tgagcgacat ggcaagctgc ggtgcccgcc cgcgcaggag tgacagggtc ttctccgcca
     4201 tgcacaaagc ggctcccgat ttttccagga tcacgccgat catcgtgcct gtaatgatga
     4261 caagccctat gtggcgcatc atattaccga agccggtatt gacagtgtcg acaagttcag
     4321 tgagaggcat atgcgccagt atgccggtaa ggtaggcggc ggcaatgagc gccagaaaag
     4381 gatggagacg gaattttgcg gttgctgcca ctataaacag aacggaaaaa agtaccacgg
     4441 cgaccagcac aggcccttga accattacaa gaccctcccc ggcagttgaa gccttttatt
     4501 tatcgtccgt ccagagcgag cgcaagcgca tctgtaatca gcggctcggc gcccggctcg
     4561 acccagcacc atttgtcttc attatagtct tccgcagagg cggcagcggt ggggatgtat
     4621 ccgggggcgc attcaccgaa acccgcaacc actaccgggg cctgagggct cattccctga
     4681 gcgaggagct gataacccac aaaactctcg ccgggaagaa tcagaaaacg ggcatgaccg
     4741 aagtccacgg cgggcacatc gatcgctctg ccctcggcga cacgtttccg ccagctgagt
     4801 gtcatggctg cgagattccg gtgaaaagtc ggtgcacttt cattggcaag tgttgccagc
     4861 gcatcttctt ccgaataccc gccgaaattc ttgggctcaa gataaagcgg tgctttacgg
     4921 aagatcacct gctccagggg ataacacacg gtgttttccc aggcggcttt catgccctca
     4981 tacatccgct cggcaagtac ggggcggttc tccggggcgc cgtcattaaa ttttccggca
     5041 gtggtgtcgc cgctgcagcc ggtaaaatac atctgaaata catccggcat ttccgcctgc
     5101 cgttttgccc gcgccatccc gacaaaatca gcggatacac caccctgacc gtaataactc
     5161 atgggatggg tggagtacgc gctcagtgcc gccagcggcc ggtcaccgtc ccagaaacta
     5221 agggttttca gccagggatc aatgataccc tcgtcctttt cactgagttt gggatctcgg
     5281 gtcgcactgc ctctgccgta agtgacggtg ccgtcatcga gaacgacccg gcggttggag
     5341 gtgacctgct tcacctttgc ctgaccgatg ccgtaatggg ttacgggtct gggcgatttc
     5401 aatgcttctt tcagagccgc tgccgtccgc tccacacacg cacgggcaaa atcgctgtca
     5461 cacagagcgc caggaagccc tgcctcgtcg agaagagact gagcagcata atcgatgacc
     5521 ggagcatcat gctgatgcac actggtatgc agaacccggt cgggtgtggt acctgccgct
     5581 tctgccagag cactgcgcca gtggtcatag gcatcattgc gcacttcgca ccagtcaacc
     5641 gtcacccata cgacaggttt ttccggaccc agcagcacca cgcccagcgc ataaagagga
     5701 tcaacgattt ctgaagcagg tttcacgccg ccgcccatac aggcatgccc gacgggaatg
     5761 gtgatatcgg tctggaaagt ggcgatctgc cagggagcag gtgcggcaag catagcggtg
     5821 agcacgagaa gagaactgat catgagcggc tcctcaaaag agatggtcgt taaaaatctt
     5881 ttcctattgt atctctttgg ttgtttcaaa tgcgatttta caggtggaaa aattacagat
     5941 atgaccggca ccttttcaaa aatttgattg aatcctgtcg atgcagtagc atgaagatat
     6001 aatcagatgt gaggaattcc ggtatgtatg agtcctgtgg cagacgtaca tttctgacgc
     6061 agactgcgtt gtccttaggg gcactcccgg cattgcttca ggtaacagcg caaactgccg
     6121 agtcgtcccg ccccaatttc ctttttcttt tcgtagacga ctaccgtgct gacgccattc
     6181 atgccctcgg ttgtaatgcc atccggactc cgaacattga ccgtctggta cggcagggta
     6241 ccgcctttac caattgctac aatcagggcg gctggcatgg agccatctgc atggcaagcc
     6301 gtgccatggt aatgaccggc agaaatctct gggataccca tgctctcgat gcgtcgcttc
     6361 cggaacaggc agcggcggga aagctctggc cgcagcggat gcaggcttcg ggctaccgca
     6421 cattcatgtc aggcaagtgg catgtgaatt gcaacccggc aaaagtgttt cagcgggcac
     6481 gtcatgagcg gcccggcatg ccacccgata tcgaaagcca gtacaggcgg ccggaagagc
     6541 aaaagcctga tcccttcgat cctgccgata caacacaggg cggttatttc accggaggaa
     6601 aacactggag cgaagtagtc gccgatgacg gggtcgcttt cctccgaaaa gaagcaggcg
     6661 atgagccttt tttcatgtat cttgctttca atgcgcccca cgacccccga cagactgcgc
     6721 acgcctggct ggatcagtac acagcggacg cactcccgct gccacccaac tttctgcccg
     6781 gctatcccta caaggatgaa atcggctgtt cggcggatat gcgggatgaa cggctggcgc
     6841 cttttccccg tacacctttt gcgatccgga cccatctgcg cgagtattat gcgattatca
     6901 ctcatcttga tgcgcagatc ggcaggattc tggatgccct ggagcggagc ggcagagcgg
     6961 aaaatactta tattattctg gccggggaca acggactttc catcggctca cacggattca
     7021 tgggcaagca gaatatgtac gagcacagca tgaaagtgcc gctgatcatg agcgggccgg
     7081 gaatcgctgc cgaccggcgc tgtgatgcgc tggtatatat gcaggatttt gtgccgaccc
     7141 tgctggagct tgccgcagtg gctccggaaa cacccatgga tttcagaagt ctgctgccgc
     7201 tgctgcgcgg agaggaaagc agcctgcgcc cccatattta cggtgcttat atgatgctgc
     7261 agcggatggt gcgcggggaa cggtacaaga tgatttatta tcctaccgtg agccggtacc
     7321 gtctttatga tctgcaggaa gatccttttg aattgcagga tcttagtgag gtgccggaac
     7381 atggcgcaac attgaaggaa ctgcaggagc agctgcagcg gctgcagaaa gaaatgcatg
     7441 atcccctgag cggatcagtg tcataaaagt tagaaggagt gagtgatgct tacaagacgt
     7501 acttttctca agcagaccgg cgctgcggcg gcaatggccg ctttgctgcc gaggctctgc
     7561 catgcacagc agatgcccaa tatcatctgg atctgctgtg atgaccatgc gcagaatgcg
     7621 atcagcgcct atggcggccg gctggcggaa gtggcaccga cgcccaatat agaccgtatc
     7681 gccagagaag gtgcgctctt tcgcagcagt tttgtgacca actccatctg cgggcccagc
     7741 cgtgccgtgg tgctcaccgg tctcttcagc cataaaaacg gatacaaaga caaccggagc
     7801 cgttttgatg gcagtcagca gaccttcccg aaactcctgc aacaggcggg atacgaaacg
     7861 gcgctcttcg gaaagtggca tctccacagc gatcccacgg ggtttcatca ttgggaaatt
     7921 ctgcccggtc agggcgatta ttacaatccc gatttcatta ccaaagaggg gcagaagcga
     7981 aatgaaggtt atgtgaccga cctcatcacc gacaaggcgc tgcactggct tgagcacgaa
     8041 agggacaacg gaaaaccctt catgatgatg gtccagcaca acgcacccca ccgcgaatgg
     8101 ctgccgtcgc cgaagcatct gcgcacctat gaggataccg tttttcccga gccggagacc
     8161 cttttcgacg attatgccac ccgcggtacc gctgctcata aacaggatat gactatcgaa
     8221 aaaaccatgc ggcccggcgc tgatctgaaa ctcttccgta aagaagatga aggaagcgct
     8281 gaatggaaag acatctttga acggatgacg gcggaacaga gggctgactg ggaggatgca
     8341 tacagagcgc gcaatgagga atactggtcg ggcaagtatc aggggaaaga actggtccgc
     8401 ttcaagtacc agtgctatat cagggactat atgcgctgtg tggcttctgt ggatgataat
     8461 gtgggcagag tgctggatta tctggagaca agcggactgg ataagaatac gctgattttc
     8521 tattcatccg atcagagctt ttatctcggg gaacatggct ggttcgacaa acgctttatg
     8581 tatgaggaat cgctccgtac accgctgctc gcacgatggc gcggtgttat caaaccggga
     8641 agtgagattg atgccatggt gcagaatctc gactatgccg aaacttttct tgcagcggca
     8701 ggggcggctg tccccgccaa tatgcagggc accagtatgc tgccgcttct ccgcggtgag
     8761 acagccgacg actggcgcaa gtccatttac taccactact acgaaggcga aaaagccgta
     8821 cacgccgtat acaaacatta cggcgtccgc acggaacgct acaaactcat gtatttctat
     8881 accctcgacg aatgggaatt ctacgacctg caggaagacc cccacgaact ccgcaacgaa
     8941 tacaacaacc ccgactacgc cgaggaaatc caaaaagcga agcaggaact tgaacgcctc
     9001 cgccagcttt atgaagtccc ggaggacgac gaataggagc ctgcgacaat gaagcatccc
     9061 ctgggcactt ttttccacgc agccgccctg attctctttt tggcaggggg cggtgtcctc
     9121 atttactacg agatctacac gctttacctc gcctggcagc actggagcat ctggggcatc
     9181 ctcgcagcaa tcatccttcc cggtatcttt aatatcgccc tctttttcta tgtctgcttt
     9241 tctgccggca ccctcttcac gtactacacc ttcctcagta tcatctggct tgcacctgtt
     9301 atccccgcca tcatcctcgc cgccttcgcc gaaggcttta aaaagaaaga ggggtgagtt
     9361 aatggtcatt tactaatagc ttcttcttag tacaagcata gaaataataa tgtttagagt
     9421 gtgttcttca a
//
LOCUS       DAOPWB010000119         9351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7401_length_9351_cov_6.132423, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000119 DAOPWB010000000
VERSION     DAOPWB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7401_length_9351_cov_6.132423"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..944)
                     /locus_tag="PKN92_10225"
     CDS             complement(57..944)
                     /locus_tag="PKN92_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNZ49211.1"
                     /translation="MKSLKVAVVFFIIGAVCGTLGGGYMGYKIGSADNSIDRAPVATV
                     VPVQEINRNKPKPAPQTQPAATAATEENAVAPVEAVTEAPAVTAQETVAAENQATEAV
                     VETADETPAAATAEETDASASTETFTIHADEDSDNSVIEWVGYKSLMNQRLSMKGGFM
                     HFDGEITVVDNAPDESYVELTIDLEQIFSENVILTKVLKGEFFFDVAHYPTAHFISSN
                     VEPMDDGKFMVTGNLTMHDTTVGVQFPAVIERRGDKAYAAGEFAIDRTHWNIGYEKFE
                     DFLILNEVFVSFEILADPA"
     gene            complement(1050..2528)
                     /locus_tag="PKN92_10230"
     CDS             complement(1050..2528)
                     /locus_tag="PKN92_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ49212.1"
                     /translation="MKTNMNRRNFLMTAGATAGFAIASGYSPFSYAQNEKVRVGCIGT
                     GGQGTFHIRDGLTGTPDIEIVAVCDVFLPHQREAIKLAQLANAGITITPEKRLTDEEK
                     QMASAATRPNAYYDYKEMLEKEELDAVVIATPLATHFPITMDCLNAGKWVFCEKTLVK
                     TIEEGRDLITRCHELGRWVQVGHQRRYNPKYNMAMGLAYDDNMIGRITHITAQWHRNH
                     YWRRVLPKDYVLNDEEKKFITDLEHHLNWRIYADSSEGLFTELATHQTDISNWFLKKV
                     PSRVHAFGGLDYWRDGRTTDDNILLAFEYEESSNDPGFMPIDQRSQFQKLRELSKPYT
                     VRSVYSSILANAKRGASELIQGDRGTFELTEGKCLMFGEDVVTQKDEGQSAEEMAAAT
                     TAGSTRQVSTEELLNGKELLADVALLTPDVYQFRAFANCIKNGGVPRSNQMVGFTTAI
                     TAIAAIKSRREGGVVEIDPSWYSFDFEVPSFYDFGKWEEEIS"
     gene            complement(2525..3577)
                     /locus_tag="PKN92_10235"
     CDS             complement(2525..3577)
                     /locus_tag="PKN92_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNZ49213.1"
                     /translation="MTAITALVAFHTGGTADDVSEEAAQKSSLPVVAVRHIAMATDFE
                     LIIYGNYEDEDPEALREYGREAFAAVDRLEDEISIWRPGSAASRINRDAETCPVKVPS
                     DMITLLQESQKFYDLTGGVFDVTVGPLLELWGFYRKEGKLPGEAVLEKTLASIGLKYV
                     HLNREESTVFFERTGMRVDFGGIGKGMALDRAAAVLRHQGIKNARLNGGSSTIIGLGA
                     PPGKTGWTVDISSPYNSDAGSSFATVTIKDEAMSTSSASERYLEIDNVRYGHILDPRT
                     GWPVANGVISATAIVPEGLASDALSTAFFVMGEEAVRTFCNIHKEVRCVLLVESGAET
                     KVLFFNFEDEHVKETR"
     gene            3709..4920
                     /locus_tag="PKN92_10240"
     CDS             3709..4920
                     /locus_tag="PKN92_10240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNZ49214.1"
                     /translation="MPEVIIRGVSSPEEHEKARSLIFTTFYQDDPDGFHRMHQFLNLI
                     PDFPDDHTRILLIDNQIAACLTLFTYTIRLGESRLKMGGIGHVATAPAFRRCGYAARL
                     MDDTLRYMAEKKYHVSTLFGIVDFYHRWGYSSVLPEYSIDIAVDEALACTAANGRERK
                     IKAGDLSAIQRIHLKNDTGSAGSIIRFAGHYNSRWDVWKNARVLMDDQGKIVAYYLGN
                     VYGNQYRITEAGILDGLWSGPLLHACARRGKDSFVSNLLFHLPPAHPLAASFLLYQSD
                     HKTTVFRNRHGMMTVVDLQETLEAMVADWEYRIGNLFESALCAEVTLLIDKEAYRIRC
                     NRGFIDVAKTTGNNKVSLNRIELIHLLTGYRYPEEILSTKKHVLPRSALLLLDALFPK
                     RHPYIWTLDRF"
     gene            5032..5301
                     /gene="rpsT"
                     /locus_tag="PKN92_10245"
     CDS             5032..5301
                     /gene="rpsT"
                     /locus_tag="PKN92_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNZ49215.1"
                     /translation="MANIKSQKKRIKTSELQRQRNVSQRSKMKTLEKRATAKISAGSV
                     EDAAAEVKAAISAIDRACSKGVLHRNQAARRKAILQNSLNRMSDV"
     gene            5560..6153
                     /locus_tag="PKN92_10250"
     CDS             5560..6153
                     /locus_tag="PKN92_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal RNA adenine dimethylase
                     domain-containing protein"
                     /protein_id="HNZ49216.1"
                     /translation="MAWLTFMKEVILENKTTGALGPSSKELAVAITSLANLSESKVIV
                     EYGSGDGVFTQEIGRRKNHDAFFMAMEVNEILVKATRKRCPGVTVVHDGAQNVRAHLE
                     KYGHSCCDTIISGLPWTRFDAALQDEILNATYDILCPGGKFLTFAYASSPLFPGGRRF
                     FKEKLLQRFPKVERVGPIWKNIPPCHVYIACKPLQQL"
     gene            complement(6169..7437)
                     /locus_tag="PKN92_10255"
     CDS             complement(6169..7437)
                     /locus_tag="PKN92_10255"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNZ49217.1"
                     /translation="MEMLAAIVQKIIMVAGQMSPYLLFGFLVAGVLHVTISPEWMRRH
                     LGGKGILPVFKSVLLGVPLPLCSCGVIAVTASMRKQGAGKGASVGFLISTPQTGVDSI
                     LATWGMLGPFLGLFRPFVALVTGMVGGMAVALTSDDEEGGQVTTSDADLLGERPPRTV
                     GEIVRYGLVELPRDIAKPLLIGVVIAGAVTALIPEKMLAPYLGGGLFAMVIMTFFGMP
                     VYICATAAIPLALSFIHMGASPGAALAFLIAGPAANSASIATVWEMMGKKATVIYVMT
                     VGLGAILSGLFFDTFLSSANLIHAAMNHHHAEGGAVKNVLFGVLMALVLINSIWGSAL
                     RYKIRSLFRSGTKEEESEMEVTIQGMTCMRCVGAVEKRLAEVPGIKEVHVELPTGRAR
                     LYGKIPSVQKIFDAVQELGYEVKVAEKKTS"
     gene            complement(7579..>9351)
                     /locus_tag="PKN92_10260"
     CDS             complement(7579..>9351)
                     /locus_tag="PKN92_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNZ49218.1"
                     /translation="PGSGPFPVFMTQETHRSWAQIALQRGYIACIYAGADTRDDTDTF
                     CEAWPDCDWSRLLRRGWAAGRCLDYLETIPEADTSRTALAGHSRNGKSSLMGGAVDER
                     IAVIISSSSGVGGSMPSRYCGEHQMAEGIDHITRTFPEWFHPRWRFFAGNEHKLPFDL
                     HQLICLSAPRPVLLSIAVNDPVEHTWAMQHSYLEAQKVFKLYGAEEHLRILWRQGGHE
                     TWPEVIEQYLDWCDLHFGRDAYVFPERFVYPWDWEAWRKNARTDFSSAEFQKPAAGDR
                     EKNDLKEVVQHFMGEASPHVPVENTDYGIMKDNQLALLSRTNVAGGLERDQIVFGSYI
                     NGDIYLPKGSLEKQKKLPAVLYLPSFSTPKGYAAAYKRGGDVFNTLAQSGFAVFCYDP
                     IGTGRRVEEAEHFYDRFPKWSLLGKKVRDAQDALNAMVQLPYIDGNRIWVVGYESGAL
                     EAMHLAAVDERPAGYAFVCPPLPFYLDTDEAETGGVRRWAQERMLLPQLGLFIGREKE
                     IPYDLDEIMRTIAPKPLVLLTPYYDRFASPEKVDIYYESLKRCYGSTDSPEHLVRMTP
                     LKFNHFDESMQTLLLEALMPLVKK"
BASE COUNT         2410 a         2461 c         2136 g         2344 t
ORIGIN      
        1 atggtagcat atctcctgcc cggcaaagcg ttcaagggta aataaacagg tggttatcag
       61 gccggatcag ccaaaatttc gaaggataca aaaacctcat tcagaattaa aaagtcctca
      121 aatttttcat aaccgatgtt ccagtgtgtc cggtcaatag caaattcacc ggcagcatag
      181 gctttatccc cgcgccgttc aataaccgcc gggaattgaa cacctaccgt cgtgtcatgc
      241 atggtaaggt ttccggtaac catgaacttt ccatcatcca tgggctccac attggaactg
      301 ataaaatgcg ctgtcggata atgagcaaca tcaaagaaga actcaccttt cagcactttc
      361 gtaaggatta cgttctcgga aaaaatctgt tccagatcga ttgtaagttc gacataactt
      421 tcatccggcg cattgtcaac gactgttatt tcgccgtcaa aatgcatgaa gccgcccttc
      481 atggacaaac gctgattcat gaggctctta tatccaaccc actcaataac ggaattgtcc
      541 gaatcttcat cagcgtgaat agtaaaagtc tctgtgctgg cggaagcatc ggtttcttca
      601 gccgttgctg ctgccggtgt ttcgtctgct gtctcaacca ctgcttcagt cgcctgattt
      661 tcagcggcta ctgtttcctg cgctgtcact gcaggcgctt cggttactgc ttcaaccggg
      721 gcaaccgcat tctcttcagt ggcggctgtt gctgcaggct gcgtctgcgg cgcaggcttc
      781 ggtttatttc ggttgatttc ctgcacaggc acaaccgtgg ctaccggagc acgatcaatg
      841 gaattgtctg ccgagcctat tttatagccc atgtatccgc cgcccagtgt cccacacaca
      901 gcaccgataa taaaaaatac caccgctact tttagagatt tcatgaaaac gtactccgac
      961 cggcattgat agtgcctgct tctggtttat gaacctctct gcttcagcca tctgaagcag
     1021 agaggtatta cttgtttgtt aacgaacaat caggaaattt cttcttccca tttcccgaaa
     1081 tcatagaaac tgggaacttc aaagtcaaaa ctgtaccagg acgggtcaat ttctacgaca
     1141 ccgccttcac gccgcgattt aatcgcagcg attgcagtaa ttgctgtagt aaacccaacc
     1201 atctgattgc ttcggggaac gccgccgttt ttaatacaat ttgcgaaggc ccggaactga
     1261 tatacatcgg gtgtcagaag agcaacgtca gcgagaagtt ctttgccatt aagcaattct
     1321 tccgtgctga cctggcgggt gcttccggct gtcgttgcag cagccatttc ttccgcactc
     1381 tgaccttcgt ctttctgagt caccacatct tcgccaaaca tcaagcattt gccttcggtt
     1441 aattcaaagg tgccccgatc gccttgtatt aattcagatg caccgcgttt ggcatttgca
     1501 agaatactgg aatagacaga acgaactgtg taaggtttgc tgagctcacg cagtttctgg
     1561 aactggcttc gctggtcaat gggcatgaat ccgggatcat tgctgctttc ttcatactca
     1621 aaagcaagca ggatattatc gtccgtggtg cgtccgtcgc gccaataatc caaaccgccg
     1681 aaagcatgga ctctgctggg tacttttttc aggaaccagt ttgaaatatc ggtctggtgt
     1741 gttgccagtt ccgtgaacaa tccttcggaa ctgtccgcat aaatacgcca gttaaggtga
     1801 tgttcgaggt cggtgataaa ctttttctct tcatcattca acacatagtc tttcgggaga
     1861 acccggcgcc agtaatggtt gcggtgccac tgtgcggtta tgtgagtaat gcgcccgatc
     1921 atattgtcat cgtaagcaag gcccatggcc atattatatt tgggattgta ccggcgctga
     1981 tgtccgacct gtacccagcg tccgagttca tggcagcgtg taatcaggtc acgtccttct
     2041 tcaatggttt tgaccagtgt cttttcacag aacacccatt ttcctgcatt caggcagtcc
     2101 atcgtaatag ggaaatgggt agcgagaggc gttgcaataa cgactgcatc caactcttct
     2161 ttctcaagca tctctttgta gtcgtagtag gcgttgggac gcgtcgcagc acttgccatc
     2221 tgtttttctt catcagtcaa ccgtttttcc ggcgtaatcg tgatgcctgc attggcaagc
     2281 tgcgccagtt tgatggcttc acgctgatga ggcaaaaaca cgtcgcaaac agcaacgatt
     2341 tcaatatccg gagtacctgt caggccgtca cggatatgaa aggtgccctg accgcccgtg
     2401 ccgatacagc caacccgcac cttctcgttt tgggcatagg aaaacggcga gtagcctgaa
     2461 gcaatggcaa agccggctgt agcgcctgcc gtcatcagaa agttccgtcg gttcatgttc
     2521 gtcttcatcg ggtttccttt acatgctcat cttcaaaatt gaaaaaaagt acttttgttt
     2581 ctgcgccgga ttcgacaagt aaaacacagc gcacctcttt atgtatattg caaaaagtgc
     2641 gaaccgcctc ttcgcccatg acaaagaaag cagtgcttaa agcatcgctt gccagacctt
     2701 caggaacgat ggcagttgca ctgatgacac cgtttgccac aggccatccg gttcggggat
     2761 ccagaatatg accgtagcga acattgtcta tttctaaata acgttcagaa gccgacgaag
     2821 tagacatcgc ctcatctttt atcgtcactg tagcaaaaga actgcctgcg tcgctattat
     2881 acggtgaact gatatccaca gtccaacctg tcttgcccgg aggggcaccg agtccgataa
     2941 tcgtgctgga tccgccattc agacgtgcgt ttttaatccc ctgatgacgc agaaccgcag
     3001 ccgcccgatc gagtgccatg ccttttccga taccaccgaa atcaacacgc atgcctgtac
     3061 gttcaaaaaa gaccgtactt tcttccctgt tcagatggac gtacttcaaa cctatggacg
     3121 caagcgtttt ctccagcacc gcttcgccag gcagttttcc ttcttttctg taaaatcccc
     3181 agagttccag aaggggacct accgtcacat cgaaaacacc gcctgtcaaa tcgtaaaact
     3241 tttgagactc ctgcaacagt gttatcatat ctgacggcac ttttacagga caggtttcag
     3301 catcgcggtt aattctggac gcagcacttc ccgggcgcca gatacttatc tcatcttcca
     3361 gacgatccac tgcagcaaac gcctctctgc cgtactcacg caacgcctcg ggatcttcat
     3421 cctcgtaatt accgtaaata atcagttcga aatccgtcgc catggcgata tgcctgacag
     3481 ccacaaccgg caaggaagac ttttgggccg cttcctcaga cacatcatcc gccgttccac
     3541 cggtgtgaaa cgcaacaagt gcggtaatgg ctgtcaaaac aaaagaaact cttaacatag
     3601 cgtataataa tgatttcctg tactataaga tcataatctt cttttagcgt gtgtgcgccc
     3661 tgccacccaa aacgaattct gcatatcata gcattaggag cctttttcat gccagaagtg
     3721 attatccgcg gcgtctcttc ccccgaagaa catgaaaaag cgcggtcatt aatatttacc
     3781 accttttatc aggatgatcc cgatggtttt catcggatgc atcaattttt aaatctgatt
     3841 cctgattttc cggacgacca cacacgcatc ctcctcatcg acaatcagat agccgcatgc
     3901 cttacgcttt ttacttatac aatccggctg ggagaatcgc ggttgaaaat gggcggtata
     3961 ggacatgttg ccactgctcc cgcattccga cgctgcggtt atgcggcgcg actcatggac
     4021 gatacgctcc gctatatggc ggaaaagaaa tatcatgtat caacgctgtt tggtattgtc
     4081 gatttctatc accgctgggg atacagttcg gtgctgccgg aatactccat tgatattgct
     4141 gtcgacgaag cgctggcgtg caccgctgca aacgggcggg aacggaaaat taaggcaggc
     4201 gacctgagtg caatacaacg cattcatttg aaaaatgata ccggctctgc aggatccatt
     4261 atccgttttg ccggccacta caacagccgc tgggatgtct ggaaaaatgc ccgggtactg
     4321 atggatgatc agggaaaaat cgtcgcttat tatctgggga acgtctatgg aaaccaatac
     4381 cgtattacag aggcaggcat cctcgatggt ctgtggtcag gtccattact ccatgcctgc
     4441 gcacgccgtg gaaaagattc ttttgtttcc aatttacttt ttcatctgcc gccggcacac
     4501 ccgcttgccg cttcttttct cctgtaccaa tcagaccaca aaacaacagt gttccgtaac
     4561 cgccacggca tgatgaccgt ggttgacctg caggagacgc tggaagcaat ggttgctgac
     4621 tgggagtacc gcataggcaa tttattcgaa agcgcccttt gtgctgaagt gactcttctt
     4681 atcgataagg aagcctaccg tattcgctgt aaccgcggtt ttatagatgt tgcaaaaacg
     4741 acaggaaaca ataaggtttc tttaaatcga atcgaactga tccatcttct gacaggctat
     4801 cgctatccgg aagaaatatt atcaacaaaa aaacatgtac ttccccgatc cgcactcctg
     4861 cttctggacg ctctttttcc aaagcgccat ccttatattt ggacgcttga ccgtttttaa
     4921 ggcagggtcg caagatacac caatgacaca aagttgaatt cgccctgttc tgttgctata
     4981 atagtgacct catccacctg caactacaag actacctaca aggttatatc tatggctaat
     5041 attaaatcgc agaaaaaaag aattaaaacg agtgagttac agcgccagcg gaatgtctca
     5101 cagcgttcaa aaatgaagac gctggaaaaa cgtgcaactg ccaagatcag tgccggatcg
     5161 gtggaagatg cggcggctga agttaaagct gctatttccg ctattgatcg cgcctgctcc
     5221 aaaggtgtac tccaccgcaa tcaggcagcg cggcgcaaag ccatcctgca gaactcttta
     5281 aaccggatgt ccgacgtata acacagacgc catctgatcc cggacgatca gaaactctgt
     5341 tagcaatttt ttcgttgtag gactgagggg tcttatataa agcggacacg cacctattga
     5401 atgcgtgtcc gctatttttt ttgtgctact gggaaagggg catcagaaat ttgacttttt
     5461 gactgaaaag gcgcatactt tctatcgttc gatagaaaac cgtttacacg ctaacattct
     5521 gacaaccaaa cagcgcccct gttgtaccgg agacaagtca tggcatggct gacttttatg
     5581 aaagaagtaa ttcttgagaa taaaacaacg ggagcactgg gccccagcag caaagaactt
     5641 gccgtagcga tcacttcttt ggcaaatcta tcagaatcca aagtgattgt tgaatacgga
     5701 tccggagacg gcgtttttac gcaggaaatc ggacgtcgca aaaaccacga tgcttttttt
     5761 atggcaatgg aagtcaatga aattctggtg aaagcgacca gaaaacgctg tcccggtgtt
     5821 actgtcgtgc acgatggcgc tcaaaacgta agagcacatc tggaaaaata cggtcacagc
     5881 tgctgtgata ccattatttc cggacttccc tggacacgct tcgatgcggc tttgcaggac
     5941 gagattttaa atgcgaccta tgacattctg tgtcccggcg gaaaattttt aacttttgcg
     6001 tatgcgtcca gtcctctttt tccgggaggc cgccggtttt tcaaggaaaa actgcttcag
     6061 cgatttccaa aagtggaaag ggtaggtcct atctggaaaa acatcccgcc gtgccacgtc
     6121 tatattgcct gcaagccttt gcagcagctt taaggctaca gttgccggtc agctcgtttt
     6181 tttctcagca actttgacct catacccgag ctcctgcaca gcgtcaaaga ttttttggac
     6241 agaaggaatt ttgccgtata aacgggcgcg accggtcgga agttctacat gcacttcttt
     6301 tatgccggga acttctgcca gtcttttttc gacagcaccg acacagcgca tacaagtcat
     6361 accctgaata gtaacttcca tttcgctctc ttcttccttc gttccggatc ggaaaagcga
     6421 acgtatttta taccgcaatg cactccccca gatgctgtta ataagcacaa gcgccatcag
     6481 gactccaaac agaacatttt taactgcccc gccttccgca tggtgatgat tcattgcggc
     6541 gtggatcaga ttagcgctgg agagaaatgt atcgaaaaaa agtcctgaaa gaatggcgcc
     6601 cagcccaact gtcatgacat agattaccgt ggccttcttg cccatcattt cccagactgt
     6661 cgcaatggaa gcggagttgg cggcaggccc ggctatcaga aatgccagtg cggcacccgg
     6721 tgaagcaccc atatgaatga aactcagtgc aagcggtatg gctgcagtgg cacagatata
     6781 gacaggcatt ccgaaaaatg tcataataac catggcaaaa agaccgccgc ccagataggg
     6841 tgccagcatt ttttccggaa taagcgccgt aacagcaccg gcaatgacca cgccaatcaa
     6901 cagcggtttg gcaatatccc taggcaattc aacaagtccg taccggacta tttcgccaac
     6961 agtgcgcgga gggcgctcgc ccagcaaatc agcgtcactt gttgtcacct gcccgccttc
     7021 ttcgtcatcg ctggtgagtg caacagccat ccctccaacc atacctgtga ccagagcaac
     7081 aaagggccgg aacagtccaa gaaaagggcc aagcattccc caggtggcaa gaatggagtc
     7141 tactcctgtc tgaggtgtgg atataagaaa gccgacagaa gcccctttac cggcgccctg
     7201 cttgcgcata gatgccgtaa ctgcaatgac accgcaggag cataaaggga gcgggacacc
     7261 tagcaagaca cttttgaaga ccggcagtat ccctttaccg ccaagatggc gccgcatcca
     7321 ttcgggggaa atggtaacgt gaagcacccc tgctacaaga aaaccaaaaa gcagatacgg
     7381 cgacatctgt cctgcaacca taataatttt ttggactatc gcagctaaca tttccataat
     7441 aaactttcac tttcgcgctc aaacacattc agatatcgga tgtttgccaa caaatatctg
     7501 cgcttatcag taaggaacac gcaaaagaaa aagtttattc actttttgtt caaccagaaa
     7561 tgaaagcacc cgtgccggtt acttttttac aagaggcatg agggcttcca acagaagcgt
     7621 ctgcatgctt tcgtcgaaat ggttgaactt gagaggtgtc atcctcacaa gatgttccgg
     7681 actatctgtg ctcccatagc atctctttaa agattcgtag tagatatcaa ctttctccgg
     7741 cgatgcgaac cggtcatagt aaggggttaa cagtaccaga ggtttgggcg caatcgtccg
     7801 cataatctca tccaggtcgt agggaatttc cttttccctc ccgataaaaa gtcccagctg
     7861 cggcaaaagc atccgttcct gtgcccagcg tctgaccccg cctgtttcag cctcatctgt
     7921 atccagataa aaaggaagcg gagggcaaac aaacgcatac cctgccggcc gctcatctac
     7981 agctgcaaga tgcatcgctt ccagagcgcc gctttcgtag cctaccaccc agattctgtt
     8041 cccgtcaata tacggcaatt gcaccatggc gttgagtgcg tcctgcgcat cccgcacttt
     8101 ctttccaagg agtgaccatt tcgggaagcg gtcgtaaaaa tgctctgctt cttcgacccg
     8161 acgccctgtt cctataggat cgtagcagaa cactgcaaat cctgactgag ccagcgtatt
     8221 aaaaacatca ccgccccgtt tatacgcagc cgcatagcct ttaggggttg aaaaagacgg
     8281 cagataaagc acagccggca gttttttctg cttttccaga cttcctttcg gaagatatat
     8341 atcgccgtta atgtaggagc cgaagacaat ctgatcccgt tcaagcccgc cagcgacatt
     8401 tgtgcggctc aacagcgcca gttgattatc cttcataatt ccataatccg tgttttctac
     8461 gggaacatgg ggcgaggcct cccccataaa gtgctggaca acttctttta agtcattctt
     8521 ttcacgatca ccggctgcag gcttttgaaa ttcggcagac gagaaatcag tccgggcatt
     8581 tttccgccag gcttcccagt cccagggata cacaaaccgt tcaggaaaca cataggcatc
     8641 ccgaccaaaa tgcagatcgc accagtccag atactgttca atcacttcag gccaggtttc
     8701 gtgtccgccc tgccgccaga gaatgcgcag atgctcttca gctccgtaca gtttaaatac
     8761 tttctgcgcc tctaagtaag aatgctgcat agcccaggta tgttcgaccg ggtcattgac
     8821 agcaatgctt agaagcacag gacgcggtgc ggacaggcat atgagctgat gcagatcaaa
     8881 gggcagttta tgctcatttc ctgcgaaaaa ccgccatcgc ggatgaaacc actcaggaaa
     8941 agttcgggtg atgtgatcta ttccttccgc catctgatgt tcgccacaat agcgtgaggg
     9001 catggagcct cccaccccgc tgctgctgga aataattaca gcgatacgtt catccactgc
     9061 cccgcccatg agagaacttt tcccgttacg ggagtgtcct gctaaagcgg tacgggacgt
     9121 gtctgcctcg ggtatcgtct ccagataatc aagacagcgc ccggctgccc agccgcgccg
     9181 aagaaggcgt gaccaatcgc agtcgggcca ggcttcacaa aaagtatccg tatcgtcgcg
     9241 ggtatcggct ccggcataaa tacaggcaat atagcctcgc tgcaatgcta tttgcgccca
     9301 ggagcggtga gtttcctgtg tcataaatac cggaaaaggt ccggaacccg g
//
LOCUS       DAOPWB010000120         9307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7445_length_9307_cov_9.885538, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000120 DAOPWB010000000
VERSION     DAOPWB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7445_length_9307_cov_9.885538"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(481..1599)
                     /locus_tag="PKN92_10265"
     CDS             complement(481..1599)
                     /locus_tag="PKN92_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNZ49219.1"
                     /translation="MARKSQTGNNIYLRKDGRWEGRRRTGTENGKTVYIYIYGGSYDE
                     AVQKLDEIAALQEDLGDENSFQRIAKEWLELQKPQLKPASFSSYTNIINSYLNPKFGE
                     RQITDISRSEFTSLVRDLLEHGGITQQGLSASTVNTILTVAKKIFIYAARERELSVAD
                     IQGIPVKQVRNDTPTLSKEDQETLVQWLYDHLTPGNLGILLSLYTGMRLGEICALKWK
                     DISKEDQSIAVYQTMQRVQRLDENRTEVVVQAPKSASAYRRLPVSEEMARVLAASRKE
                     DDAYILTGDPEHFMEPRGLQRQFKRVLRACGLEDINFHALRHTFATRGIEVGFDPKTL
                     SSLLGHATVSMTLNRYVHPSMEQKQKNMNLFPELLAAR"
     gene            2179..2301
                     /locus_tag="PKN92_10270"
     CDS             2179..2301
                     /locus_tag="PKN92_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49220.1"
                     /translation="MKLLKGIGHGLRTVKDFFVVIFVVLGVWLVTKDDPNGNTG"
     gene            2343..3821
                     /locus_tag="PKN92_10275"
     CDS             2343..3821
                     /locus_tag="PKN92_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNZ49221.1"
                     /translation="MKKKLTGWLGKTIPFLCLAAFAVLFCMALSLNILPNTYVAVLAA
                     VMVVLTAVVALLTWSGKSKVRFILGTLLAVIFIFLSIAGTYYINKTAATAQKITTPTV
                     ETTEVGIFVRTEDAENFSENASGYVYGFLAELDRENTDAAIEAFNREDAFILQTTEYD
                     GLTDLADALLETKDVDAMILNTAYLDVLEEMDGYEDIMDRIVQVTYQSVKKEVEVNAD
                     SEKEAGLSGDTELSDSIFTVFISGIDSRKGLIAKSLSDVNILAVVNAETRQILLVSTP
                     RDYYVPLSISNGRKDKLTHAGIYGIDVCMDTISMLYDVDIDYFFRLNFTGFVDIVDAL
                     GGITVVSDYTFTAGNYSFVKGENQLDGTAALAFCRERHAFATGDRQRGKNQLAAIKGI
                     VNKVTEPSILMNYSSFLSALEGSFETSIPYSLISELVKAQLNDNSAWDIVSYSVDGTG
                     DSQIPYSMSQYAYVMVPDESTVETAKGLIQTVLDGEMVSDPQ"
     gene            3838..4617
                     /locus_tag="PKN92_10280"
     CDS             3838..4617
                     /locus_tag="PKN92_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNZ49222.1"
                     /translation="MQKCEEQASMNQKKTEYIDLLKLARAMWKRAWLILLATAAAGAV
                     VYLYSVFLATPMYTAQTLLYVTNRSDSASNISSSDLTAAQSLVDTYGVILTARSTMEE
                     VLEVSGTDYTVEELQNMVSSGAVDSTEVLSVTVTDADPAEAAKIANAIAEVLPEKISE
                     HIQGSSVSVVDYASVPKKQSSPNVTGNTLKGCLLGFVLACGYIVILFLMDTKIHDEEY
                     LMQTYKLPILAAVPDLSRKSSQSGYYAAPDAHEDGKRVTQR"
     gene            4614..5339
                     /locus_tag="PKN92_10285"
     CDS             4614..5339
                     /locus_tag="PKN92_10285"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNZ49223.1"
                     /translation="MSKEQKQRARERENRRQLVGENLSFEASEAYKLLRTNLTLSLPA
                     GEGACRLIGITSSVLGEGKSTTAINLAYSLGKGRKKVLLLEADLRKPVLRDAFKLPRG
                     RGLSNLLTGGCDFKEAITMNVFHPSVGVLPAGDIPPNPAELLNSTPMKTVLDTVSQIF
                     DYIIVDLPPVTIVSDALAMANKLDGMIVVVRKDYCDQRALAETMRRLEQVKAKVLGFV
                     MTHADDTEKKYKKYGYGYGYGKS"
     gene            5382..6110
                     /locus_tag="PKN92_10290"
     CDS             5382..6110
                     /locus_tag="PKN92_10290"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49224.1"
                     /translation="MFDFHSHILPGIDDGSQSVKESLNLLRESARQGVTAMAATSHFY
                     ASEYSPKEFLAKRNAAWKQLEPYLEAGMPEIKLGAEVHYFEGIAQSEDVCMLRIEGTS
                     LLLVEMPMSWWTSRMVDALLDLNSRPDTTVLLAHIERYLSMQDRKTWKLLRSEGVKMQ
                     VNASFFWNPRTRRKALGMLKKKEIHALGSDCHNMENRPPRLADAKREISARLGKEALI
                     WLRRQERCLLAPVEWETSEFRSEL"
     gene            6125..6364
                     /locus_tag="PKN92_10295"
     CDS             6125..6364
                     /locus_tag="PKN92_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49225.1"
                     /translation="MKKVIYDITDICVACGKPSPPGDMLCWECRKNANAAGMKQVQPA
                     EDAALNAVNTGDQSKPRSEKIRNWLDGKIHSALKP"
     gene            6523..7488
                     /locus_tag="PKN92_10300"
     CDS             6523..7488
                     /locus_tag="PKN92_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNZ49226.1"
                     /translation="MTRMDGRVPVSPGAGIFLKRSKLPLIGENRQAAEVSQTEKQKIG
                     IGYRFGKLTIGADTGKRKNGYTVWRCDCDCGGSIELDTRAIQRGAYKDCGCETKVTPR
                     ARDLTGMRFGKLVCIAPTDQRKYHTTVWRCLCDCDKVAYVPAQQLLSGYVKSCGGLSH
                     PPLKDFVGKRFGKLTVISYEGKRAGMHRWRCRCDCGNETVVGQTLLQTGKTKSCGCLQ
                     AEVYRENLKLVDGTSVTLLEAGKKRLISSNTSGYTGVYLQKATGKWAAQITFRGKTYF
                     LGAYEKKEDAVKARQRGEEMHDEFLEWYHSTYHQSTEKENVKSHS"
     gene            7536..8972
                     /locus_tag="PKN92_10305"
     CDS             7536..8972
                     /locus_tag="PKN92_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HNZ49227.1"
                     /translation="MKTMKHDISLRIVKYLNVILVTIPFALCWYGYYADRIVIPFYDK
                     GNWLIIAIFMVLYIVFARVYDAFLVSLNRISEMVYSQILSVLMADAMMYVILWLLMRH
                     FPNILPALAALALQIVLVVMWCVLAHKWYYAVFPPQKSMIVYDTREGLEKLIRQYGLE
                     KKFDVQRVMQVEECLDDLWVLDGTEAVFLSGIHSHERNIILKYCVEHNVRVYVIPRIG
                     DVIMSGAKQMHLFHLPVLRVGRYSPLPEYLFFKRAFDIVAAGIAVVILSPVMLVTAIA
                     IKIYDGGPVLYKQTRLTKDGRQFLVWKFRSMSVNAEQDGVARLSTGANDSRVTPVGRV
                     IRKVRVDELPQLFNVLGGSMSIVGPRPERPEIAAQYEEEMPEFRLRLQAKAGLTGYAQ
                     VYGKYNTTPYDKLQMDLMYISHPSFLEDLRICFVTVRTLFSGEESTEGIAEGQVTAMK
                     RAPQEDCGTEQEIDQIVVENGTLTGAGR"
BASE COUNT         2365 a         2108 c         2533 g         2301 t
ORIGIN      
        1 catgagtgct acccttatgt caatactttt gcacacgcct gttgctgata catttcacct
       61 ttcaccttat tcgaacatcc caagcctgat atacaacttc atataataag agagagccaa
      121 attacagtgc ttaaccacgc tgtagtttgg ctcttttttg tgcttattat cattctgacg
      181 gattcgcagc gctgcggtgc tttttgatac tttactatct tcagacaaac tttgttatca
      241 gatagttatc aattcagatg ttgacttccc gtgggttcag aggaatagat atagtctggc
      301 aacgccaaaa actgccgaaa taaggggaaa tttggcctgt aatctgtcgt ataagaggtt
      361 attatacgac acaggcccct tttccccttg atatacaggg ctttcgtcca tttttttgac
      421 ggctaattct gacggctatg acggcgaatt ttgacggcta attttcttgc ttcgtaagcc
      481 ctatctggcg gccagcaact cgggaaacag gttcatattt ttctgtttct gttccatgct
      541 gggatgcaca taccggttga gcgtcatgct cacagtagcg tggcctaaga gggagcttaa
      601 ggtttttgga tcgaagccga cctcaatccc ccgagttgca aaagtatggc ggagcgcatg
      661 aaaattgatg tcctccaatc cacacgcccg cagcactcgt ttgaactgtc tctgcaaacc
      721 gcgcggttcc atgaaatgct ccggatcgcc cgtgagaata tacgcatcgt cctcttttct
      781 gctggcagca agaaccctcg ccatctcctc tgagaccggc agtctgcggt atgctgacgc
      841 actctttgga gcctgcacaa ccacttcggt cctgttttcg tccaggcgct ggactcgctg
      901 catggtctga tagactgcaa tggactgatc ttccttggaa atgtccttcc atttcagtgc
      961 gcagatttca cccaggcgca tccccgtgta taaactgagc aggatgccaa gattaccggg
     1021 tgtcaggtga tcgtatagcc attgcaccag cgtttcctga tcctcttttg acagcgttgg
     1081 agtgtcgttt ctgacctgtt ttacgggaat tccctgaata tctgccacag aaagttccct
     1141 ttcccgcgcc gcataaatga aaatcttttt tgctacagtc agaattgtgt tgactgtgct
     1201 ggcggaaagc ccctgctgtg ttatcccgcc atgttccagt aaatcccgga caagagaggt
     1261 aaactccgac ctgctgatat ccgtgatttg cctttccccg aatttggggt taaggtagct
     1321 gttgatgata tttgtgtagg atgaaaaact tgcgggtttc agctggggtt tttgaagttc
     1381 cagccactcc tttgcgatgc gctgaaagga attttcatcc cccaaatcct cttgcagggc
     1441 ggcaatctca tccagctttt gcactgcctc gtcataggaa ccaccataga tatagatgta
     1501 aacggtcttt ccgttttcag tgccggttct acggcggcct tcccatcggc catcctttcg
     1561 gagataaata ttgtttccag tctgtgattt tcgtgccata tatcaagcac ctccttcttg
     1621 tgatgctatt attttatcgt gcagaatgaa tggactgccc agctttacag gctgtttacg
     1681 gcaatgttac gagtaaattt gagaaaattt tacaattagc cacaaattat tgggaaaaac
     1741 cacttgtatt aattcctcaa atcgtgtata ataagtaatc gagtgtaggg ttctttaagg
     1801 gcaaccgatt tgggcctttc aaatgggctg tcgtataata acctcttata cgacagtttg
     1861 gcgtctttta catctttagt ctgcgcatat tttgcgcaga ggatacagga gcacagaaga
     1921 aaacagccct tcccgcaaac agtgctgtgg gaaaggcaaa ttagatagaa gcgtactggg
     1981 ggaaatatgt atgaagaaac cttatgagaa accggcattt gtgatggaac gctttgacac
     2041 gggcgagatc atctgctccg atccggattc ggaattctgt aaaagatttc tacgggaagc
     2101 ggaggaagcc aagaagcatc ccgtccccat ctgcaccccg ctgtgcggaa gaaccggttg
     2161 cgttctggag aatgacttat gaagctactg aaagggatcg gccatggtct gcgcacagtg
     2221 aaggacttct ttgtggttat ttttgttgtc ctgggcgtgt ggctggtgac aaaggatgat
     2281 cccaacggca acaccggctg acagtgtgga aatctttggt gagataaagg aaaagggtgg
     2341 atatgaaaaa gaaattgaca ggttggcttg gaaagacgat cccttttcta tgcctggcgg
     2401 cgttcgcagt tctgttctgc atggcgctgt ctctgaacat acttccaaat acctatgtag
     2461 cagttctggc tgctgtaatg gtggtgctta cggcagtcgt agcgctgctg acatggagcg
     2521 gaaaaagtaa agtccgattt attcttggta ctctgctggc tgtgattttc atttttttat
     2581 ccattgcagg tacctattat ataaataaga ccgctgctac agcgcaaaag atcacaactc
     2641 ccacagtgga aaccaccgag gtcggtatat ttgtccgcac agaggatgcg gaaaatttca
     2701 gtgaaaatgc ttctggctat gtttacggtt tcctggccga actggatcgg gagaatacgg
     2761 atgccgcaat tgaagcattt aacagggaag atgcttttat cctccagact actgaatatg
     2821 acggcttgac tgatttggct gacgctctgc tggaaacgaa ggatgtggat gccatgatcc
     2881 tgaacacagc ctatctggat gtgctggagg aaatggacgg ttatgaggac attatggatc
     2941 gcatcgtcca ggtgacatat cagtccgtga agaaagaagt ggaagtaaat gcggactcgg
     3001 agaaagaagc gggactcagc ggagacacag aactttcaga ctcaatattc actgtcttta
     3061 tcagcgggat cgacagcagg aaagggttga ttgctaagag tctgagcgat gtcaatatcc
     3121 tggcggtggt caatgccgaa accagacaaa ttcttttggt atccacaccg agagattact
     3181 atgtgcccct gtccatctcc aacggaagaa aagataagct gacacatgcg ggtatctatg
     3241 gcattgacgt gtgtatggat accatttcca tgctctatga cgtggatata gattatttct
     3301 tccgcctgaa ctttacaggc tttgtagaca tcgttgacgc attgggcggc attacggttg
     3361 tttctgacta tacctttacc gctggaaatt actcctttgt gaagggagaa aaccaattgg
     3421 atggcactgc tgccttggct ttctgtcggg agcgccacgc ctttgcgacg ggagatcggc
     3481 agcggggaaa aaaccagctt gctgctatca aagggatcgt caataaggtg accgagccaa
     3541 gcatcttgat gaactactct tcatttctta gtgctttgga aggcagcttt gagacaagca
     3601 ttccctatag tctgatcagt gaactggtca aggcgcaact aaatgacaac agtgcgtggg
     3661 atattgtcag ctatagcgtg gatggcaccg gggactccca gatcccctac tccatgagcc
     3721 agtacgccta tgtcatggtt ccggatgagt ccacggttga gactgccaag ggactgattc
     3781 agaccgtttt ggatggagag atggtctctg atccgcagta aaggaaaccg atattttttg
     3841 cagaaatgcg aggaacaagc aagtatgaat caaaagaaaa ccgagtacat agatctattg
     3901 aagcttgcgc gggcaatgtg gaagcgcgcc tggctcattc tgcttgcaac ggctgcagct
     3961 ggagctgttg tatatctata ttctgttttt ctagccacgc ctatgtacac tgcgcaaacg
     4021 ctgttgtatg tgaccaatcg gtcggacagc gcaagcaaca tcagcagcag tgacctgacc
     4081 gccgcccaaa gtctggtgga cacctatggg gttatcctta ctgcgcgctc caccatggaa
     4141 gaagtgctgg aagtctctgg gacggactat acggtggagg aactgcagaa catggtttcc
     4201 tccggcgcag tggattccac ggaagtgctg tcggttacgg tgaccgatgc cgatcccgca
     4261 gaggcggcga agatcgccaa cgccattgcg gaggttttgc ccgaaaaaat atccgagcac
     4321 atccaaggca gctcggtttc cgtggtggat tatgcttctg ttccgaaaaa gcaatcctca
     4381 cccaatgtaa cgggaaatac attaaaagga tgtcttttgg gttttgtact ggcttgcgga
     4441 tatatcgtga ttctgttcct gatggacacc aagattcacg atgaggaata tctgatgcag
     4501 acatataagc tgccgattct agccgccgtg ccggacttga gccggaaatc ctcgcagtca
     4561 ggttactatg cagccccaga cgcacatgag gatggaaagc gggtgacgca gagatgagca
     4621 aggaacaaaa acaacgggcc agggagcggg agaaccgcag acaactggtc ggtgagaatc
     4681 tgagcttcga ggcttcggaa gcgtacaagc tattgcgtac caacctgacc ttaagtctgc
     4741 cagctgggga aggtgcctgc cgtctgatcg gcatcaccag ttcggtactc ggcgaaggaa
     4801 agagtaccac agccatcaat ctggcctact ctctagggaa aggcagaaaa aaagttttat
     4861 tgctggaagc agatctgcgg aagcctgttt tacgggatgc gtttaaactt ccccgggggc
     4921 gtggcctttc caatcttctg accgggggct gtgattttaa ggaagcaatt acgatgaatg
     4981 tgttccatcc aagcgtgggg gtactcccag ctggggacat tccgcccaac ccggcggaac
     5041 tgctgaattc caccccgatg aaaacagttc tggataccgt atcccagatt tttgactaca
     5101 tcatcgtgga tctgccgccg gtcactattg tcagtgacgc attggcgatg gcaaataagt
     5161 tggatggaat gatcgtggtg gtgcggaagg actactgcga ccagcgggcg ctggcggaga
     5221 cgatgcgccg tttagagcag gtgaaagcaa aggtgctggg ttttgttatg acccatgcag
     5281 atgacacgga gaaaaagtat aagaaatacg ggtatggtta cggatacggt aaatcctgag
     5341 cataccggaa gctggaggga acccgggaag gaggcatcgc tgtgtttgat ttccattccc
     5401 acattcttcc cggtattgat gacggaagcc agagcgtgaa ggaaagcctg aaccttttgc
     5461 gggaaagcgc cagacaaggg gttaccgcta tggcggccac ctcccacttc tacgccagcg
     5521 agtattctcc gaaggagttt ttagccaagc gaaatgcagc gtggaaacag ctggagccct
     5581 atctggaggc cgggatgcct gagatcaagc tgggggccga ggtacattat tttgaaggaa
     5641 tcgcccagtc agaagatgtg tgtatgcttc gcatagaggg aacctccctg cttctggtgg
     5701 agatgcccat gagctggtgg acaagccgga tggtggatgc gctgctggac ttaaacagcc
     5761 gtccggacac cacggtgctg ctggcccaca tagagcggta tctttccatg caggacagga
     5821 aaacctggaa actgctccgg tcggaagggg tgaagatgca ggttaacgcc agcttcttct
     5881 ggaatcccag aaccaggcga aaggcgcttg gaatgctgaa aaagaaagaa atccatgcat
     5941 tgggttctga ttgccacaac atggagaacc gaccaccacg gctggcagat gccaagcggg
     6001 aaatctccgc ccgattgggg aaagaagcat tgatatggct gcgcagacag gaacgctgcc
     6061 tgctggcgcc ggttgagtgg gagacttcgg aatttcgatc cgaactgtga aaggatagga
     6121 gcagatgaag aaggtaattt atgacattac ggatatctgt gtggcttgcg gcaaaccctc
     6181 gcctcccggc gatatgctct gctgggagtg cagaaagaat gcgaatgcgg ctgggatgaa
     6241 gcaagttcaa ccggctgaag acgctgccct gaatgctgtg aatacagggg atcaaagcaa
     6301 accccgttca gaaaagatca gaaattggct ggatggcaaa atccacagtg ctctaaagcc
     6361 ataagattat tgattatatt tcggacgaat cagcccggat atgataccaa gggcggtcag
     6421 gttctgcgaa aacgcctgac ccgccccggt gcggactgga tcatgcaccg agttttcatg
     6481 gtttgaatcc tttcggttca gccaaaacat ccagacggag tgatgacccg gatggatggg
     6541 agggtgcccg tgtctccggg cgcgggtatc ttcttaaagc gcagcaagct gccgctgatt
     6601 ggagaaaaca ggcaagcagc ggaggtgagc caaacggaaa agcagaaaat aggcatcggt
     6661 taccgctttg gtaaattgac tatcggcgcc gacaccggca agcgcaagaa tggctacact
     6721 gtctggcgct gtgactgtga ctgcggcggc tccatagaac tggatacccg ggccatccag
     6781 cggggcgcat ataaagactg cggctgcgag acgaaggtca caccaagagc cagggacttg
     6841 accgggatgc ggtttggaaa actggtttgc attgctccta cagatcagcg taaataccat
     6901 actacggtct ggcgttgcct ctgcgactgc gacaaggtgg cctatgttcc ggcacagcaa
     6961 ttactctccg gctatgtaaa aagctgcggg ggtttaagcc atccgccgct caaggatttc
     7021 gtcgggaaac gctttggaaa actcacggtg atctcctacg agggcaagcg ggccgggatg
     7081 caccgctggc gctgccggtg tgattgcggc aatgagactg tggttggaca gaccctcctc
     7141 cagaccggca agacaaaaag ctgtggctgc ctccaggcgg aggtttaccg ggagaacctc
     7201 aagctggtgg atggcacctc cgtgacgctg ctggaggccg gtaaaaagcg cctgatcagt
     7261 agcaacacca gcggctatac cggcgtgtat cttcagaaag ccactgggaa atgggcagca
     7321 cagatcacct tccgaggcaa gacctatttt ctcggcgctt atgagaaaaa agaggatgcc
     7381 gtgaaagcca gacaacgagg tgaagagatg cacgatgagt ttctggaatg gtatcacagc
     7441 acctatcatc agagtacgga gaaagaaaat gtcaaatcgc attcgtgaaa ggcaattttg
     7501 aacagagtgt ttttcatcat gctgagaaag aggatatgaa aacgatgaag cacgatatat
     7561 cgctgcgtat tgtaaaatac ctgaatgtca ttctggtgac gattccgttt gcactttgct
     7621 ggtatggcta ttacgcagac cgcatagtta tcccattcta tgacaaggga aactggctta
     7681 tcattgccat ttttatggtt ctatatatcg ttttcgcaag agtttacgat gcgtttttgg
     7741 tttctctcaa tcgtatctcg gaaatggttt acagtcagat tctttcggtt ctcatggcgg
     7801 atgctatgat gtatgtcatt ctctggctgt tgatgaggca ttttcccaat atcctgcccg
     7861 cattggctgc gctggcctta caaatcgtgc tggttgtgat gtggtgtgtg ctggctcata
     7921 aatggtatta tgcggttttc cctccccaaa agtcgatgat tgtgtacgac actcgcgagg
     7981 gactggaaaa gctcattcgc cagtacgggc tggaaaagaa atttgatgtt caacgcgtga
     8041 tgcaggtaga agagtgtctg gatgatcttt gggtattgga cggcaccgaa gcggtatttc
     8101 tcagcgggat tcacagccat gaacggaaca tcattttgaa atattgtgtg gagcataatg
     8161 tgcgagtcta tgtgattccc cgcattgggg atgtcattat gagcggcgcc aagcagatgc
     8221 acctgttcca tttgcctgtg ctgcgtgtag gacggtacag cccgctgccg gaatatctgt
     8281 ttttcaaaag agcatttgat attgttgctg ccggaattgc ggttgtgata ctctctcctg
     8341 tcatgcttgt aaccgccatc gcaatcaaga tatatgacgg gggcccggtg ctttacaagc
     8401 agacccggct gacgaaggac ggccggcagt tcctggtttg gaagtttcgg agtatgtccg
     8461 taaacgccga acaggacggt gttgcccgac tgtctaccgg tgcgaacgat tccagagtga
     8521 ccccagtggg tcgggtcatt cggaaggttc gtgtggacga gctgccgcag cttttcaatg
     8581 tacttggggg atccatgagt attgtaggcc cccgcccgga gcggccggag attgcggcgc
     8641 agtacgaaga agagatgccg gagtttcgct tgaggttgca ggccaaagcc ggattgaccg
     8701 gatacgcaca ggtttatgga aaatataaca caacgcccta tgacaagctc caaatggatt
     8761 tgatgtacat atcgcatcca agttttctgg aggatttgcg catttgcttt gtgactgtgc
     8821 gcacgctctt cagcggcgag gaaagcaccg agggcatagc ggagggacaa gtcaccgcga
     8881 tgaagcgtgc gccgcaggaa gattgcggaa ccgagcaaga aattgaccag attgtagtgg
     8941 aaaatgggac attgacggga gcaggaagat gatggggaat gaggatcctc ttcttgccgg
     9001 agagcaccga gggaatttca gcaggtcaga ccactgcaat gacacaagat attgtggatt
     9061 aagacaaagt ggacacaata tatagcgatg gagaagaaaa cgaggaaaaa actgtgcaaa
     9121 acagctagta cattttcaca gtttattcat aaataagtca cacttgaagg taagataaca
     9181 gcaacgctta atgtgcatta ttgaaagggg aagcggcttg aatgtagaag aaaacactgg
     9241 agatgtttag ctccccagtg ttttagacgg agtggcgatt aggccgactt cttgacagtc
     9301 tggcaga
//
LOCUS       DAOPWB010000121         9280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7478_length_9280_cov_5.167046, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000121 DAOPWB010000000
VERSION     DAOPWB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7478_length_9280_cov_5.167046"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2985)
                     /locus_tag="PKN92_10310"
     CDS             complement(<1..2985)
                     /locus_tag="PKN92_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HNZ49228.1"
                     /translation="MKIRSLLLFILALTPLTGNTAELWRLGATDRNWNDFAIAGDHQA
                     FMRHFAAGVSLTVNQDNPAEAWPYIHPGPEDAWAGGRPQVFKINFSLDAIPESACRFT
                     LALAATHPQSPPRIMISVNGHASDIMQLAAGSSDAALSNPEKGNAVSPSIVFPSAWLQ
                     LEHNQIEIANLAGSWMLYDSMVLEDGVSPVPEITRFEAESTLLFKEVDGAPRQAVRVR
                     ITNQGLAGEGTLSLAGTEEATQPVTVAHGDSSLMLLVPPFTKKEWRQIVLETGNTKQL
                     VGFDASPERQWTLFVAPSAHTDIGYTDLQEKCMALHADNACSALDEIAKKPLFKWNLE
                     VFAQYDWFKQQYPERVEELDRQIQNGKIGLTAFYLNMLTGLCSGSEMMHLLAPAQATA
                     RRLGVPVQMASMNDIPAATGTLPMFLRHAGVRYFAEALNEDRGPVFRHCDPEMNQSPF
                     WWEAPDGSRVLTIFTRTYFQAMQIHMHENTVAMEEALPAFLKTFIRYDYPGDALFING
                     AYLDNWGMNPVYGKTADEWNRSWASPKIKLATAEEYFRYIEENFAQDLPVYKGTMGGF
                     WEDGAASTAKETAQVRWSKNLVSALEKWEALAAIRGLEAEDGAKEDALWSNILYYDEH
                     TWGSAVSISDPDNPQSTGQWARKASYSNKAYEESATAAKTRGLAAITRLAGLSGNSGT
                     GVLTVVNSFSWDRDIPVSLPAGTFAEAIVLKDVKKGKKIVPQQTSAGGFYFVAEDVPA
                     LGWRSYEILTGGSDNAAPLLTVSEADPYSFVSRTFAFHINPQTGGLDRLTNLETGDEW
                     VDQTSGYHLNQFIHVEGGSQGIIYSNLETFTDFRMISPTQASVSLVENGPERAVLQIT
                     STGGFSPVDTQLIVHKDGTLDFINTIHKTETLKKEGGYFAFPFNMNTPDRATAYMEAP
                     YGVFAADQDYMPGACREWHAVQHFAAVDDGTRSAYIAAPDTPLFTVGDVNRGVWPNRL
                     KGNRHILFAYVYNNYWHTNYKAS"
     gene            complement(3092..4240)
                     /locus_tag="PKN92_10315"
     CDS             complement(3092..4240)
                     /locus_tag="PKN92_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNZ49229.1"
                     /translation="MTTEKEPDISRRDFVCRSATAGLPFLGTRKIRQERRCMRTPAPP
                     HAPVQSRAAVAAVRGTDLKAMAREALDAFGGARAIVGEGETVFIKPNFGAFGMVKYNP
                     VLKGDCTKPEIVAAVAEECLKAGAKKVTIGDAGQAYRYDWNRIAFLDGSSTMEKEAGL
                     LALKYPGRLELACLNADSPGWDPVPSYTRLNKIYVSSMITRADRVISLPVLKSHRWTH
                     ITGAMKNFVGATSTRHYGLGIQWRFILHSAGIEQSFLDIVREVKPDFTLMDCSLCCEG
                     NGPHVCHPYWGETVDMRVRLGSWLVLAGRDLAAVDATAARVISHDVSAVTHLQMAYNQ
                     GLGQIQEDKIDLLGASLSELQVPWKKASLTKGFMEVLLPGFNLLTGCR"
     gene            complement(4430..5455)
                     /gene="waaF"
                     /locus_tag="PKN92_10320"
     CDS             complement(4430..5455)
                     /gene="waaF"
                     /locus_tag="PKN92_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNZ49230.1"
                     /translation="MIQEHTSILALAPNWLGDAAMATPAFRALKQLHKNAFLTIAGSA
                     AVCALLEESPWADALHPLPVRAGLPVLRREAHSLKNAGRDLIVVFPHSFRSALLARLI
                     GGKERIGYARNGRSFLLTHPVEPYREEGRITPIYMSEEYLNLVAPAGAIGDNAGLELG
                     VSAVLLKETKERLHGTTPLVGIAPGAAFGPSKRWIPEAFAKTADLLFEKRGARCVLIT
                     GPNEKDLAQEIRAAAKHPLLEMPRADAGIAGLKAVTASLDLLICNDSGPRHIAVAFHV
                     PVVCIMGSTSPRYTDSPYEKGTVIREPLPCSPCQKAVCPLGHHLCMRLITPERVFSAA
                     DALLPSA"
     gene            complement(5507..6034)
                     /locus_tag="PKN92_10325"
     CDS             complement(5507..6034)
                     /locus_tag="PKN92_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49231.1"
                     /translation="MNTKDYWKLFTNDQSPADFVWQYGRGDSIVAVEAFLQSYPNFMG
                     IMRTGSWKETFASEEPLHRDTLRFALRFFLEERRSDWENKIEEYRYLEKQKTAETKAQ
                     QDARALAEALTRIEEDAHKSLETGVATITDLETPERLSEYTESTDAMSDSEVSPEDDY
                     SPDLYPNAPDETAGI"
     gene            6218..6625
                     /locus_tag="PKN92_10330"
     CDS             6218..6625
                     /locus_tag="PKN92_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49232.1"
                     /translation="MFSILRVKCPHCGVEGQLMIPQQEALIVGPCPECNEFVVIFAGT
                     VLSLEKSIMQSDNREEIYNHLHQVVSEHLKSCLNQFFENAEAPRKKIRRRRIRIHPRR
                     EASEITEQELQDFINNDLPKLDNPDYFRTIFGG"
     gene            complement(6653..7207)
                     /locus_tag="PKN92_10335"
     CDS             complement(6653..7207)
                     /locus_tag="PKN92_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49233.1"
                     /translation="MKQNPILWLLILLPAALSGCSGVCANDDDEQDRTVTITAMEDTL
                     GYTLSAPFYQFSGTLTKESSKDPWKLKADFYLPNSGCYVSDPEILIAESYPEQVKITL
                     TVNPAPPGMIILPAFFVKSAEAEIQASDEAVFSVVLVDKIRRHHDDLKRRLPAPPGAP
                     NQQPRRPAPKPVPPKLPPGAYTPQ"
     gene            complement(7234..>9280)
                     /locus_tag="PKN92_10340"
     CDS             complement(7234..>9280)
                     /locus_tag="PKN92_10340"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49234.1"
                     /translation="LWRCLHQKKRDFFLAQMPSGNTPLLFVKHAPGGFSHQLTQYYGR
                     YARPGGGVFVLEAPGASMRVRALAESHFPDGSFMHPEISTDGGRILVAFCATEIGAGG
                     SFDGNPGHFFNLYEISADGAEAKRLTRGNFDDFAPKFLPDGDVIFISTRRGGWHRCGT
                     PGCEVYTLARMQADGQNIQVLSFHETQEWDPVVLHDGRVVYTRWDYVDRHAVHYQQLW
                     VTRPDGTNPAIFYGNNTLNPVGVWETRPVPGSDKVIATAAPHHGMTAGSIILIDTSKG
                     MDDLEPLTRLTPEVPFPESEAVLLPHWRSELRPDPPAVTPEMERWPGQCYKNPWAFSE
                     KLFLAAYSFDPLIGEPKGNFANTFGIYLVDASGNKELLYRDLNISSLSPLSMQPRKNP
                     PVLPSALQPDAAPEGTFYLQDVYESDPRLPEEKITHLRLVQVLPKSTPGANNPTVGAA
                     NASPGRQVLGTVPVEEDGSAYFKAPSGIGIAFQALDASGQAVQVMRSLTYLQPGENVS
                     CVGCHETRSSTPPTVSVRSQALRRAPSEITPAPDGSRPLSYPILVQPVLDKHCLSCHD
                     AEATQSELKLTGAPEGRYTQSYNALIPHVSFSYWGRGQFPAGNCEPGTQPGFFGARGS
                     HLTQLLLNGHYDVKLDKDEWERLITWMDANALFYGTFNKKDQARQQMAERIAGPELE"
BASE COUNT         2097 a         2346 c         2612 g         2225 t
ORIGIN      
        1 cgatgccttg taattggtgt gccagtagtt gttgtagaca taggcgaaaa gaatgtgtct
       61 gttgcctttg aggcggttgg gccagacgcc ccggttcaca tcgcccaccg tgaaaagcgg
      121 cgtgtcagga gcggcaatat aagcggagcg tgtgccatcg tccacagcgg caaaatgctg
      181 caccgcatgc cattcccggc acgcaccggg catataatcc tgatccgcag cgaagacgcc
      241 gtaaggcgct tccatatagg cagtagctct gtcgggcgta ttcatattga agggaaaggc
      301 gaagtagccg ccttcttttt tcagggtttc ggttttgtga atggtattga taaaatcaag
      361 cgttccatct ttatggacaa tcaattgcgt atcgacgggc gaaaagccgc cggtactcgt
      421 aatctgcaac acagcacgtt cagggccgtt ttcaaccagc gatacagagg cctgcgtagg
      481 ggaaatcatc ctgaaatcgg tgaaagtttc cagattggaa tagatgatac cctggcttcc
      541 accttccaca tgaataaact gattgagatg atagcctgat gtttggtcta cccactcgtc
      601 tccagtttcc agattggtaa gccggtcaag cccgcctgtc tgcgggttga tatggaaggc
      661 aaaagttcgg ctgacgaagc tgtagggatc cgcctcggag acggtgagca ggggagcggc
      721 gttgtccgag cctcctgtga gaatctcgta gctgcgccaa cccagagcag gtacgtcttc
      781 cgctacaaaa taaaaaccgc ctgcagaagt ctgctgaggt acgattttct ttcctttttt
      841 tacgtctttt aaaacaatcg cttcagcaaa agtccccgca ggcaatgata caggaatgtc
      901 tctgtcccag ctgaaggagt tcacgaccgt gagcactccg gttccggagt ttccggagag
      961 gccggccagc cgggtgatgg ctgcaagtcc ccgtgttttc gcagcggttg cgctttcttc
     1021 ataggcttta ttggaatagg acgccttgcg cgcccactgt cctgtgcttt gcggattgtc
     1081 cggatcactg atgctgacag ccgagcccca ggtgtgctca tcataataaa gaatattcga
     1141 ccagagcgca tcctctttcg ctccgtcttc ggcttccaga cctctgattg cggccagtgc
     1201 ttcccatttt tccagtgcgg acaccaggtt tttcgaccac cgcacctgtg ccgtttcttt
     1261 tgccgttgag gcggctccgt cttcccagaa accgcccatg gtgcctttgt atacaggaag
     1321 atcctgagcg aagttctctt ctatataccg gaaatattcc tctgcggttg ccagcttgat
     1381 tttcggagaa gcccagctgc ggttccattc atcggcagtt ttcccgtaaa caggattcat
     1441 accccagtta tccagatacg caccgttaat aaaaagagca tcgcccgggt agtcgtatcg
     1501 gataaaggtt ttgagaaaag cgggcagtgc ttcttccatg gcgactgtgt tttcgtgcat
     1561 atggatctgc atcgcctgaa aataggttct ggtgaaaatg gtgagcaccc gggacccgtc
     1621 aggcgcctcc caccagaagg gcgactgatt catttcggga tcacaatgcc tgaacaccgg
     1681 gcctctgtcc tcattgagcg cttcggcaaa gtagcggacc ccggcatgac gcagaaacat
     1741 gggcagggtg cctgttgccg ccggaatatc attcatggat gccatctgta caggtacacc
     1801 aagccgacgg gcagtggcct gagccggcgc aagaagatgc atcatttcgc tgcccgaaca
     1861 cagtccggtc agcatattca gataaaatgc cgtcagcccg attttgccgt tctgaatttg
     1921 cctatccagc tcctcaaccc gttcggggta ttgctgtttg aaccagtcat actgagcaaa
     1981 tacttccagg ttccatttga aaagaggctt ctttgcaatc tcatcgagag cggagcaggc
     2041 attgtccgca tgaagcgcca tgcatttctc ctgcagatcg gtgtagccga tatcagtgtg
     2101 agcagagggt gcaacaaaaa gcgtccactg tcgttcaggg cttgcatcga acccgacaag
     2161 ctgcttggta ttgcctgttt ccagtacgat ctgacgccac tcttttttgg tgaaaggcgg
     2221 taccagcagc atgagtgacg aatcgccatg ggcgacggtg acaggctgtg ttgcttcttc
     2281 agtgccggca agggacagcg ttccttctcc cgccagcccc tgattcgtga tccgtacacg
     2341 gactgcctgc cgcggagcgc catcgacttc tttaaagagc agggtgcttt ccgcttcaaa
     2401 tcgtgtgatt tcgggcaccg gggaaacacc atcctccaga accatactgt catacagcat
     2461 ccaggagcct gcaagattgg caatctcaat ctggttgtgt tccaattgga gccaggcgga
     2521 agggaagact atcgaggggc tgacagcatt gcctttttcc ggattgctga gcgccgcgtc
     2581 actgctgccc gccgcaagct gcatgatgtc gctggcatgg ccgttgacgg aaatcatgat
     2641 acggggcggc gactgggggt gtgttgctgc cagtgcgagg gtgaaccggc aggcgctttc
     2701 cggtatggca tcgagcgaaa aatttatttt aaagacctgc ggccgaccgc ctgcccaggc
     2761 gtcttccggg ccgggatgaa tatagggcca tgcttctgca ggattatcct gattcacagt
     2821 caggctgact cccgccgcaa agtgccgcat aaaggcctga tgatccccgg cgatggcaaa
     2881 gtcgttccag tttctgtctg tcgcacccaa acgccacaat tccgccgtgt tgcctgtgag
     2941 cggggtgagt gcaagaataa aaagaaggag agatcttatc ttcatggaat ttcctttccg
     3001 tagtgatcga agcaaacttc attcattgta cggcaaagta aaatcccgtt caatgagcag
     3061 acgcgtcggg gtttccgggg gaggtgtttt ctcagcggca gccggtcagc aggttgaagc
     3121 ccggcagaag cacctccata aaacctttgg tgagggaggc ttttttccac ggaacctgaa
     3181 gctcagacag ggaggctccc agaagatcaa ttttatcttc ctgaatctga cccagcccct
     3241 gattatatgc catttgcaga tgtgtgacag cggatacatc gtggctgata acacgggctg
     3301 ccgtggcatc tactgcagcg agatcacgac cagccagcac cagccagctg cccaggcgga
     3361 cacgcatgtc caccgtctcg ccccagtaag gatgacacac atgagggccg ttcccttcgc
     3421 agcaaaggga gcagtccatg agtgtgaaat cgggcttgac ttcacggaca atatccagaa
     3481 aactctgttc aattccggca ctatgcagga tgaagcgcca ttggatgccc agtccgtaat
     3541 gacgggttga agtggcgccg acaaagtttt tcatggctcc ggtaatatgt gtccagcggt
     3601 ggcttttcaa aacagggagg gaaataactc tgtctgcccg ggtgatcatg gaggaaacat
     3661 agatcttgtt taatctggta taagacggta cgggatccca tccgggcgag tcggcattca
     3721 gacaggccag ttctagtctg ccagggtatt ttaaagcaag aagtccggcc tctttttcca
     3781 tggtcgaaga gccgtcgagg aaagcgattc ggttccagtc gtaacggtag gcctgccccg
     3841 catcgccgat agtgactttt tttgccccgg ctttcaggca ttcttcagcc acagcagcca
     3901 cgatctcagg ctttgtgcaa tcgcctttca gcaccgggtt gtactttacc atgccgaaag
     3961 cgccgaaatt gggcttgata aaaaccgttt ctccttcgcc gacaatcgcc cgggctccgc
     4021 cgaaagcatc cagcgcttca cgagccatcg ccttgagatc agtgccccgg acagctgcca
     4081 cggcagcgcg cgattgaaca ggtgcatgcg gcggggcagg agtacgcata cagcgccttt
     4141 cctggcggat ttttctggtg cccaggaagg ggagccctgc tgtcgccgaa cggcagacaa
     4201 agtcacgtcg ggaaatgtcc ggctctttct cagtagtcat aaaggcactc ctgtactgta
     4261 tttcgggagg aaagacaggc gtgatgacgt aaccaaaata ttcgtaaatt aaacagtatt
     4321 caatattcag tatatagcaa gtgtggcaat tccgcaagca ttttctgaat ctttgaaagg
     4381 tatcacgtcc cgtcttatga aaccggatca gtagggagaa gtgaagattt tatgcggagg
     4441 ggagcagcgc gtcggcggca ctgaagacgc gctccggcgt gatcagacgc atacagaggt
     4501 ggtgccccag agggcagacc gctttctggc aggggctgca ggggagcggt tcccggatga
     4561 ctgtcccttt ttcataagga ctgtccgtat agcgcgggga ggtggaaccc ataatgcaca
     4621 caacagggac atgaaaggca accgcaatat gccgtgggcc gctgtcgttg caaatcagca
     4681 gatccagcga tgctgttacc gctttgagcc cggcgattcc tgcatcggct cggggcattt
     4741 ccagcagggg gtgttttgcg gctgcccgga tttcctgcgc cagatctttt tcattcggtc
     4801 cggtaataag gacacatcgg gcacctcttt tttcgaaaag caagtctgct gtttttgcaa
     4861 acgcctcggg tatccagcgt ttactgggac cgaaggcggc gccgggagcg atccctacaa
     4921 ggggtgttgt gccgtggagc ctttcctttg tttctttcag cagtactgcg gaaacaccca
     4981 gttccagtcc cgcattatcg ccgatagcgc cggcgggagc cacaagatta aggtattctt
     5041 ccgacatgta aattggtgta atacgcccct cttcccggta gggttccaca ggatgtgtca
     5101 gcagaaagga acgcccgttc cgggcatagc caatccgttc ttttcctccg ataagccttg
     5161 ccagcaaggc ggaacggaaa gagtggggga agacaacaat gagatcgcgg ccggcgtttt
     5221 tcagtgaatg cgcttcacgg cggaggactg ggaggcctgc ccggacgggg agaggatgga
     5281 gggcatctgc ccagggcgat tcttccagca gggcacaaac agctgctgat ccggcgatgg
     5341 tcagaaaagc gtttttgtgt aattgcttga gggcgcgaaa ggcaggcgtt gccatggcag
     5401 cgtcacccag ccagtttggc gccagtgcga gaattgatgt atgttcctga atcatccggt
     5461 tacagccccg tctctatgcc ggaaaaagtg tggtacataa ttaaaatcag atgccggctg
     5521 tttcatccgg tgcgttcgga tacaaatcag gactgtaatc atcttccgga ctcacctcgc
     5581 tgtcggacat ggcatctgtt gattccgtat attctgacaa gcgttccgga gtctccaggt
     5641 ctgtaatagt cgcgacacct gtttccagag atttatgcgc atcttcttct atacgggtaa
     5701 gcgcttccgc cagcgcccgg gcgtcctgct gtgcttttgt ttcagcagtt ttttgttttt
     5761 caagatagcg atactcttcg attttgtttt cccagtcgct gcgcctttct tcaagaaaaa
     5821 agcgcagagc gaaacgaagc gtgtcgcgat gcaaaggctc ttcggaagca aaagtctctt
     5881 tccaactgcc tgtccgcata attcccataa agttggggta actttgcaga aaagcctcga
     5941 cggcaacgat actgtctccc cggccgtatt gccatacaaa atcggcggga ctttggtcgt
     6001 tggtgaataa tttccagtaa tcttttgtat tcatacctgt attgtatcag agtagtgctt
     6061 ccgattcaga gtttgtcttt gtcttttcgg acagaatcaa tgaatagcga cttaataata
     6121 cacaaaaaca aagaaaagcc ataaaatcgc ctgtagatca aagatattta tctgtggtat
     6181 aatataaatg ggtagaaaca cagaatgagg tgatatcatg tttagtatac tgcgtgtaaa
     6241 atgccctcat tgcggagtcg aggggcaatt gatgattccg cagcaggaag cgctcattgt
     6301 ggggccctgt ccggagtgca atgaatttgt tgttattttt gccgggacag tgctttctct
     6361 tgagaaaagt attatgcagt ccgataaccg cgaagaaatc tacaaccatc ttcatcaggt
     6421 agtgtctgag catctgaaaa gttgcctgaa tcaatttttt gaaaatgcgg aagcacccag
     6481 aaaaaagatt cgtcgacgcc ggatccggat tcatcccagg cgtgaagcct cggagatcac
     6541 ggaacaggag ctgcaggatt ttattaacaa cgatcttcct aaactcgaca acccggatta
     6601 ttttagaacc atcttcggtg gctaaatgcg gatgctcagg aggggcaagg ggttattgtg
     6661 gcgtataagc gccaggagga agcttaggcg gaacgggctt tggtgccggt ctgcgtggtt
     6721 gctggtttgg tgcgcccggg ggtgcgggga gtcttctttt taaatcatca tggtgacgcc
     6781 gtattttgtc aacgagcacg acggaaaaga ccgcttcgtc ggaggcttgg atttccgcct
     6841 ctgccgactt cacaaagaag gcaggtaaga tgatcatgcc gggaggagca ggattgacag
     6901 tcagcgtgat tttcacctgt tccgggtaag actctgctat gagtatctcg gggtcggaaa
     6961 catagcagcc tgaattcggc agatagaagt ctgctttcag tttccaggga tctttggacg
     7021 attcctttgt tagcgttccg gaaaactggt agaacggtgc ggagagagtg taccccaggg
     7081 tgtcctccat tgccgtgatg gttactgtgc gatcctgttc atcgtcgtcg ttggcgcata
     7141 cccccgagca gccggagagt gctgcaggga gcagaataag cagccagagg ataggatttt
     7201 gtttcatcgg agattctcct gattcgttgt gttttattcg agttccggac cggctatgcg
     7261 ttctgccatt tgctgtcttg cctggtcttt tttgttgaat gtaccgtaga aaagagcgtt
     7321 ggcatccatc caggttataa ggcgttccca ttcatcctta tccagcttca catcgtagtg
     7381 tccgttcaga agcagctggg tcagatggct gccccgggca ccgaaaaaac cgggctgagt
     7441 gccgggttca cagttgcctg cagggaattg accgcgcccc cagtaggaaa aagatacatg
     7501 agggatgaga gcgttgtagg actgtgtgta gcgtccttca ggggcaccgg taagcttcag
     7561 ttctgattgc gtcgcctctg cgtcatggca gcttaggcag tgtttgtcga gaaccggctg
     7621 cacgagaata gggtaactta acggtctgga gccatccggg gcaggcgtga tttcagaggg
     7681 tgcgcgccgc aatgcctgag agcgcaccga aacagtggga ggcgtgctgc tccgggtttc
     7741 atggcatcct acacaggaaa cattttctcc tggctgcagg taggtaaggc tccgcataac
     7801 ctgaacggcc tgtccggatg cgtccagtgc ctgaaaagca atgccgattc cggacggtgc
     7861 tttaaagtaa gcggagccat cttcttcgac cggtaccgta ccgagcacct gccttccggg
     7921 ggatgcgttg gcggcgccga cagtagggtt gttagcaccg ggtgtggatt tgggcagcac
     7981 ctgaaccagc cgcaggtgcg tgattttttc ttcgggaaga cgcggatcac tttcgtaaac
     8041 atcctgcaga tagaaggtgc cttccggcgc agcatcgggc tgcagggcgg agggcaatac
     8101 aggcgggttt ttccggggct gcatggaaag gggcgagagg ctggatatgt tcaggtcacg
     8161 gtagagcagt tctttatttc cggaagcatc gacaagatag atgccgaagg tattagcgaa
     8221 atttccttta ggctcgccga tgagcggatc gaaactgtag gcggcgagga agagcttttc
     8281 tgagaatgcc cagggatttt tgtagcattg tcccggccat ctttccattt ccggggttac
     8341 ggcgggggga tccggacgca gctcactgcg ccagtgcggc aaaagcacgg cctcgctttc
     8401 cgggaagggc acttcagggg tgagccgtgt cagcggttcc agatcatcca tgcctttgct
     8461 tgtatcgatc agaataatgg atcccgcagt catgccgtga tgtggtgcgg ctgttgcaat
     8521 cactttatcg cttccgggca cgggtcgagt ttcccagaca cccacggggt tgagtgtgtt
     8581 gtttccgtaa aaaatggcag ggttcgtgcc gtcgggtctg gttacccaca gttgctgata
     8641 gtgaacagcg tgacggtcaa catagtccca gcgggtgtag acgacccttc cgtcgtggag
     8701 gacaacgggg tcccattcct gcgtttcatg aaaggaaagc acctgaatgt tctgtccgtc
     8761 cgcttgcatg cgggcaaggg tgtacacttc gcagcccggt gtgccgcagc ggtgccatcc
     8821 gccacggcgt gtagaaataa aaatgacatc gccatcgggc agaaacttag gcgcaaaatc
     8881 atcaaagttg ccacgggtta agcgttttgc ctctgcgccg tctgcgctga tctcgtaaag
     8941 gttaaaaaaa tgacccgggt ttccgtcaaa agagccgcct gccccaattt ctgttgcgca
     9001 gaaagcgacc aggatgcgcc cgccatccgt cgagatttcc ggatgcatga aactcccatc
     9061 gggaaaatgg ctttcagcaa gcgcacggac tctcatggag gcacccggcg cctccagaac
     9121 aaatacaccg ccgccgggtc gtgcataccg cccataatat tgggtcaact gatggctgaa
     9181 tccgcccgga gcgtgtttta caaaaagaag aggcgtgttg cctgagggca tctgcgcgag
     9241 aaaaaagtcg cgctttttct gatggaggca tcgccagagt
//
LOCUS       DAOPWB010000122         9055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7689_length_9055_cov_5.689222, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000122 DAOPWB010000000
VERSION     DAOPWB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7689_length_9055_cov_5.689222"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..469
                     /gene="rpsJ"
                     /locus_tag="PKN92_10345"
     CDS             161..469
                     /gene="rpsJ"
                     /locus_tag="PKN92_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNZ49235.1"
                     /translation="MSSDSKIRLKLQAYDHRLLDKSINQIVTAARSTGSDVRGPIPLP
                     TQMSKYTVLRSPHVDKKSREQFESRTHKRVLDILKPSSNTVDALMKLALPAGVDVEIK
                     "
     gene            530..1165
                     /gene="rplC"
                     /locus_tag="PKN92_10350"
     CDS             530..1165
                     /gene="rplC"
                     /locus_tag="PKN92_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNZ49236.1"
                     /translation="MLKGLLGKKLGMTRLFTEEGLWIPVTLLQAGPCTVVQRKTADRD
                     GYEAVQVGYGDKKESRCTKPLREHFKAAGVEPKKVLREFKVDNDSELKPGDMIGSDIF
                     KKGDRVDVSGLSKGRGFAGAMKRHNFAGGPNTHGSRFHRRPGSVGASATPAEIVKGKK
                     MPGQMGNCRVTVQNLEIIDVVHEKNLLVIRGAVPGANGGMIMVKHSVKGAR"
     gene            1168..1794
                     /gene="rplD"
                     /locus_tag="PKN92_10355"
     CDS             1168..1794
                     /gene="rplD"
                     /locus_tag="PKN92_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNZ49237.1"
                     /translation="MVTLRVVNMQGAEQAPIEASSAVFDVEVSDTLLHDVVVGLQANK
                     RQGTHKTKTRGEVSGGGAKPFRQKGTGRARRGSSREPGLKGGGVVFGPVPRSYRTSLT
                     VGMKRQALCGVLSNRVKAERLCVLSGLAFDAPKTKAFAEMVEAIDADARRTLFVVAEH
                     NPVVLLSSRNIPNVAVRTASDLNVLDVLNATRIVLQQEALPKLEERLA"
     gene            1791..2090
                     /gene="rplW"
                     /locus_tag="PKN92_10360"
     CDS             1791..2090
                     /gene="rplW"
                     /locus_tag="PKN92_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNZ49238.1"
                     /translation="MTLEYHKIIDRPIVTEESQILRENGNHYVFRVNPAANKKQIGEA
                     VEEMYRKEEIKVVAVRTMNCRGKSRRQFGTRLSGRRAHWKKAIVTLRDGDSIEFI"
     gene            2105..2932
                     /gene="rplB"
                     /locus_tag="PKN92_10365"
     CDS             2105..2932
                     /gene="rplB"
                     /locus_tag="PKN92_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNZ49239.1"
                     /translation="MPIKRYKPYTPSRRHMTVSDFSELSAVKAEKKLLKPKKRISGRN
                     NMGRVTLRRRGGGSKRLYRVIDYRCNKDGIPGTVATLEYDPNRTARIALINYADGEKR
                     YMIANTALKVGQKILSGDKADFEPGNTMELGRMPLGSVVYNVELTPGKGGQIARSAGN
                     KCQLLAKEGKFVVLRLPSGEMRRFFSECRATLGTVSNEDHANIALGKAGRTRWLGRRP
                     KVRGVAMNPVDHPHGGGEGRTSGGRHPVSPWGVPTKGRKTRKKNNRSNQFIIRRRNA"
     gene            3002..3265
                     /gene="rpsS"
                     /locus_tag="PKN92_10370"
     CDS             3002..3265
                     /gene="rpsS"
                     /locus_tag="PKN92_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNZ49240.1"
                     /translation="MSRSLKKGYFIDDHLLRKVDKQQRERDRRVIKTWSRRSTIIPDM
                     VGLTLAVHNGKSFLPVFVTENMVGHKLGEFAPTRTFRSHAGKK"
     gene            3281..3628
                     /gene="rplV"
                     /locus_tag="PKN92_10375"
     CDS             3281..3628
                     /gene="rplV"
                     /locus_tag="PKN92_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNZ49241.1"
                     /translation="MLTARAKVKNIRISARKARLVADMIRGKRVLEARDILEFSLKRS
                     AEPIRKLLDSAVANVEMQAREVRRRVDTDDFKVNTIMVDEGRTFYRHQPMARGRAGRI
                     RHRSSHVTLTITE"
     gene            3640..4455
                     /gene="rpsC"
                     /locus_tag="PKN92_10380"
     CDS             3640..4455
                     /gene="rpsC"
                     /locus_tag="PKN92_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNZ49242.1"
                     /translation="MGQKIHPFGFRLGVIQNWSSIWYAGKDYSELLHKDLQIREYVKK
                     RLENDPDNPKAEVGKVVIERVGRKTKVFIFTPQKAYAVGSSGDRVDKLRMELQKLTDH
                     DVEIEIKQIEVAELSAQLVADRLASQLERRVSFRRAMKKAMQSTMRMGAKGIRIRIAG
                     RLNGAEIARAEQSREGSVPLHTLRANVDYGTSTSRTTYGAIGVKVWIYLGDIMPGERI
                     DDLAPEKEAQDSGRSRTFRGNRDDQAGGKSGRGDRRGGRGSRGGRGSDGASPK"
     gene            4520..4930
                     /gene="rplP"
                     /locus_tag="PKN92_10385"
     CDS             4520..4930
                     /gene="rplP"
                     /locus_tag="PKN92_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNZ49243.1"
                     /translation="MLMPKRVKYRKQQRGRRMGAAKGGYSLEEGEFGLKALQDGWITS
                     RQIEAARVAINRHLRRGGKVFIRVFPDKPITKKPAETRMGKGKGAPDQWVAVVKPGRI
                     MFEIEGVPETLAREAVRLAGFKLPIKTRFVSRMQ"
     gene            4961..5176
                     /gene="rpmC"
                     /locus_tag="PKN92_10390"
     CDS             4961..5176
                     /gene="rpmC"
                     /locus_tag="PKN92_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNZ49244.1"
                     /translation="MKAKELREKTNEALEQMLKECRDNIVNFRFQLATGVVENVRRAR
                     EARKDIARIKTIIKEREMAEAKRTTES"
     gene            5173..5457
                     /gene="rpsQ"
                     /locus_tag="PKN92_10395"
     CDS             5173..5457
                     /gene="rpsQ"
                     /locus_tag="PKN92_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNZ49245.1"
                     /translation="MSTDVTQEIKDDKGNRKERVGLVVSTAMDKTITVAVDRVKEHRL
                     YNKALRRTKKYKAHDAENTCNVGDTVRIRETRPLSKTKRWRLVEIIERAK"
     gene            5476..5844
                     /gene="rplN"
                     /locus_tag="PKN92_10400"
     CDS             5476..5844
                     /gene="rplN"
                     /locus_tag="PKN92_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNZ49246.1"
                     /translation="MIQIYSTLNVADNSGARKLRVIQVMGGSKRRYASLGDIVTASVR
                     EVIPNAAVKKGDVVKAVIVRVKQDTQRPDGTVIRFDSNAAVLINAQKEPRGTRIFGPV
                     PRELREGGFLRILSLAPEVL"
     gene            5858..6169
                     /gene="rplX"
                     /locus_tag="PKN92_10405"
     CDS             5858..6169
                     /gene="rplX"
                     /locus_tag="PKN92_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNZ49247.1"
                     /translation="MYIRKKDIVVVTSGRYKGRRGRVLEVYPKKDTLLVEGVNIRKHH
                     TKPGRGNQTGGIVEREAPIHISNVMGWCEEKQQPSQIQMKKLADGRRVRVWKLTGEVL
                     D"
     gene            6237..6782
                     /gene="rplE"
                     /locus_tag="PKN92_10410"
     CDS             6237..6782
                     /gene="rplE"
                     /locus_tag="PKN92_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNZ49248.1"
                     /translation="MVARLKEHYRKEIVGALTNEFNYKNVMEVPCLVKVVVNMGVGDA
                     IADAKNMDAAIAELAQITGQKAVIRKARKSISNFKLRAGANIGCMVTLRGDYMWEFVD
                     RLFNIAIPRIRDFRGLPPKAFDKQGNYTLGIREQTIFPEINLDKVSRVRGMNVTFVIK
                     NSNSAEESYALLKHLGMPFAG"
     gene            6836..7141
                     /gene="rpsN"
                     /locus_tag="PKN92_10415"
     CDS             6836..7141
                     /gene="rpsN"
                     /locus_tag="PKN92_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNZ49249.1"
                     /translation="MAKLSKKEKHKRQLKLVEKYKEKRASLKATMIDPELSLEERLAA
                     GRALAKLPRDSSPVRLRNRCKLSGRPRGYLRKFGLSRIALRELAHEGKIPGVTKSSW"
     gene            7172..7570
                     /gene="rpsH"
                     /locus_tag="PKN92_10420"
     CDS             7172..7570
                     /gene="rpsH"
                     /locus_tag="PKN92_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNZ49250.1"
                     /translation="MSMSDPIADLLTRIRNGLQAQKPSVDVPRSQMKVAICRVLKEQG
                     YIAGFEELDNTPQGTLRIALKYLPDRRPVLQGVRRISKPSLRVYMAHSELKPVRSGLG
                     ISIVSTSKGVMTGKQARAENVGGELICEVW"
     gene            7620..8159
                     /gene="rplF"
                     /locus_tag="PKN92_10425"
     CDS             7620..8159
                     /gene="rplF"
                     /locus_tag="PKN92_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNZ49251.1"
                     /translation="MSRIGKLPVPVPAGVTCTLNGSHLKVTGPKGSLEASFSPEMTMT
                     LADGQLNVMRPSDAKEHKALHGLTRTLANNMVVGVSEGFKKTLLIEGTGYRASLQGKN
                     LNLVIGYSHPVVVEPPEGITLEVEGTSTIHVTGINKELVGQVAANIRKIRAVEPYKGK
                     GIRYQNEFVVRKESKTTGK"
     gene            8215..8568
                     /gene="rplR"
                     /locus_tag="PKN92_10430"
     CDS             8215..8568
                     /gene="rplR"
                     /locus_tag="PKN92_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNZ49252.1"
                     /translation="MAHTNPKIIQQKLRAERIRKKISGTPERPRLHVGRSLKHIYAQL
                     IDDTTGRTLATASSLALKIDGGNIQGAKEVGKSIGEKAKAIEVTQVSFDRGGHLYHGR
                     VKALADAAREAGLQF"
     gene            8616..>9055
                     /gene="rpsE"
                     /locus_tag="PKN92_10435"
     CDS             8616..>9055
                     /gene="rpsE"
                     /locus_tag="PKN92_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNZ49253.1"
                     /translation="MNRKAELTNEFKEDDSNVQHVVKIYRVAKVVKGGRRFNFSALVV
                     VGDRNGNVGAALGKAAEVPEAIRKGTEKAWKNLINVPIVATTIPHEITGRLGAARVLL
                     KPASRGTGVVAGGAVRAVVDAAGYQNILTKSLGSDCVTNIVWATL"
BASE COUNT         2467 a         2094 c         2499 g         1995 t
ORIGIN      
        1 aaggcggtcg tacggtcggc gccggtgtgg tcactaaaat tatccagtag tctgagaaat
       61 ggtgcaggca cagtgcctgc tccttgaaat tgattgtaaa acagcaaagt tgcgtccggc
      121 gtagttgtgg cgcagtcgtc atctgacagt ggagaatatc atgagctctg actctaaaat
      181 cagacttaaa ttacaagcct atgatcaccg cttgcttgac aagtctatta accagattgt
      241 tacggcagcc cgcagcacag gctctgacgt acgcggtcct atcccgcttc ccacccaaat
      301 gagcaaatac actgtgttgc gcagcccgca tgtggataaa aaatcccgtg agcaattcga
      361 aagccgtacg cataagcgag tgctggacat tcttaagccc agttccaata cagtggacgc
      421 tctgatgaaa ctggcgctgc cggctggtgt ggatgtagaa atcaaatagc aaccgagcct
      481 ggcggacggg tgccttccgg tactgatgtc cgctgtgagg ataagaatga tgctgaaagg
      541 tttgttggga aaaaaactgg gcatgacccg actcttcacc gaagaggggt tgtggatccc
      601 tgtcactttg cttcaagccg ggccgtgcac cgtggtacag cgcaaaaccg ctgaccgcga
      661 tggctacgag gcggtccagg ttggttatgg tgacaaaaaa gaaagcagat gcaccaagcc
      721 ccttcgtgag catttcaaag ccgcaggtgt tgagccgaaa aaggtgcttc gggaatttaa
      781 agttgataat gacagcgaat taaagcccgg agatatgatc ggcagtgata tcttcaaaaa
      841 gggtgatcgt gtagatgtat ccggtctgag caaaggccgg ggcttcgccg gggcaatgaa
      901 acgccacaat ttcgcgggcg gtcccaacac gcacggctcc cgttttcacc gtcggcccgg
      961 ttccgtgggc gcaagtgcca caccggcgga aattgtgaag ggcaagaaaa tgcccgggca
     1021 gatgggcaat tgccgtgtaa cggttcagaa tctcgaaatt attgatgtgg tccacgaaaa
     1081 gaacctcctg gttatccgag gcgctgttcc tggtgccaat ggcggaatga tcatggttaa
     1141 acacagtgtg aagggagcca gataatcatg gtaaccctca gagttgtaaa catgcagggt
     1201 gcggagcagg caccgattga agccagcagt gccgtctttg acgtagaagt aagcgatacc
     1261 ttgcttcatg acgtggttgt ggggctgcag gccaacaaac ggcagggcac gcacaaaacc
     1321 aaaacgcgtg gtgaagtttc cggcggtggt gccaagccct tccgtcagaa aggcactggt
     1381 cgtgcccgtc gtggcagcag tcgcgagccg ggacttaaag gtggcggtgt tgtttttggg
     1441 cctgttcccc gcagctatcg cacaagtctt actgtcggta tgaagcgcca ggcgctgtgc
     1501 ggcgtattga gcaatcgggt gaaagccgag cgtttatgcg ttctttcagg gcttgctttt
     1561 gatgcaccga aaacaaaggc tttcgcggaa atggtcgaag caatcgatgc ggatgcccgt
     1621 cgtacccttt ttgtcgtagc agagcataac ccggtagtgc ttttgtcctc acggaatatt
     1681 ccgaatgtgg ctgtaagaac cgcttccgac ctcaatgtgt tggacgtgct gaatgccact
     1741 cgaatcgttc tacagcagga agcgctgccc aaacttgagg agcggctggc atgacccttg
     1801 aatatcataa gattatagat cggcctatcg tcactgaaga atcacagatt ctcagagaaa
     1861 acggcaacca ctatgtgttt cgtgtcaatc cggcagccaa taaaaagcag atcggcgagg
     1921 ctgttgagga aatgtaccgg aaagaagaaa ttaaagtggt cgctgtgcga accatgaact
     1981 gtcggggcaa atcccgccgt cagtttggaa cgcgtctcag cggtcgccgt gcccattgga
     2041 aaaaagccat cgttaccctg cgggacggtg actccattga attcatctga cggagatatt
     2101 actgatgcct atcaaacgtt ataaaccata tacaccgtcc cgccgtcaca tgaccgtatc
     2161 ggacttctcc gagttgtctg ctgtcaaggc agagaaaaag ctcctgaagc cgaaaaagcg
     2221 gatcagcgga agaaacaata tgggtcgggt tacgcttcgt cgtcgcggtg gcggcagcaa
     2281 aaggctgtac cgcgttattg attaccggtg caataaagac ggtatccccg gaaccgttgc
     2341 cacccttgaa tacgatccta atcgtacggc aagaatcgcg ctgattaact atgccgacgg
     2401 cgaaaagcgt tatatgattg ctaacactgc cctcaaagta ggacagaaga tcctcagcgg
     2461 tgacaaggcc gattttgaac ccggcaatac catggaactg ggtcgcatgc ctctgggtag
     2521 tgtcgtctac aatgtcgaac ttacccctgg aaaaggcgga caaattgcaa gatcggcagg
     2581 gaacaaatgt cagcttctgg cgaaagaagg caaatttgtg gtgctgcgcc tgccttccgg
     2641 agaaatgcgt cgcttcttca gcgagtgccg cgccacgctg ggcacagtca gcaacgaaga
     2701 tcatgccaat attgcgctgg gcaaagcggg aagaacccgc tggctcggac ggaggcctaa
     2761 ggttcgcggt gtggcaatga acccggtaga ccatccccat ggcggcggtg aaggtcgcac
     2821 ttccggcggt cgtcatcctg tgtccccctg gggcgtaccc accaaaggac gtaagacacg
     2881 gaagaaaaac aaccggtcca atcagtttat tattcgtcgg agaaatgcat aacagcaggc
     2941 taagtgcctg acaactgcaa tatgatgcct gaaacaggca aaaccaataa ggagtaaaca
     3001 ggtgtcccgt tcgttaaaga aaggctattt cattgacgat cacctgctgc ggaaagtaga
     3061 caagcaacag cgtgaaagag accgaagggt tattaagacc tggtcacgcc gttccacgat
     3121 cattcccgat atggtgggtc tgaccttggc ggttcataat gggaagtcgt tcctccctgt
     3181 attcgtaaca gaaaacatgg tagggcataa actgggtgag ttcgcgccta cacgcacttt
     3241 ccgttctcac gcgggcaaaa aataacagga gtcttcagcc atgcttacag cacgggcaaa
     3301 agtaaaaaat atccggatct ccgccagaaa agcgcgtctg gtagctgata tgattcgtgg
     3361 caaaagggtt ttagaagcac gggatattct ggaattctct ttaaaacgtt cagcagagcc
     3421 catccgcaaa ctgttggatt cggcagtggc aaacgttgag atgcaagcca gagaagtgcg
     3481 gcgccgtgtt gacacagatg attttaaagt caacacaatt atggtggacg aagggcgcac
     3541 cttttaccgc caccagccta tggctcgcgg acgcgcaggt cgtatccgtc atcgcagcag
     3601 tcatgtaacc ctgaccatca cggaatagag gagatactca tgggtcagaa gattcatcct
     3661 ttcggttttc gtctgggcgt tatccagaac tggtcatcca tctggtatgc cggcaaagac
     3721 tattctgaac tgctgcataa agatttgcag attagagagt atgttaaaaa acgtctggaa
     3781 aacgatcccg ataatccgaa agccgaagta ggcaaggtgg ttatagagcg ggtcggccgt
     3841 aagaccaagg tgttcatttt cacacctcaa aaggcttacg cagtcggcag cagcggagac
     3901 cgcgtggata agctgcggat ggaacttcaa aagcttaccg accatgacgt ggaaatagaa
     3961 attaagcaga tcgaagttgc agaattaagc gcacagcttg tggcagaccg tcttgcttcg
     4021 cagttggaac ggcgtgtttc tttccgacgt gcaatgaaaa aagcgatgca gagtaccatg
     4081 cgcatgggcg caaagggtat tcgaattcga attgccggca gattaaacgg cgcagaaatc
     4141 gcccgtgcag agcaaagccg ggaaggcagt gtgcccctgc atacattgcg tgccaatgta
     4201 gattatggca cctctacaag ccgtaccacc tacggtgcaa tcggggtgaa agtgtggatt
     4261 tatctgggcg acataatgcc tggtgaaaga atagatgatc ttgcccctga aaaagaagcg
     4321 caggatagcg ggcgcagccg gacattcagg ggtaatcgtg atgatcaggc tggcggaaaa
     4381 tctggtcgcg gtgataggcg cggtggacgc ggttcccgtg gcggtcgcgg ttccgacgga
     4441 gcttctccta agtaacaaac gaataatcct gaaaatttgc acaatcgtgc cacaggcaca
     4501 tagtggagac tgataagtca tgttaatgcc aaaacgtgta aaatatcgta aacagcagcg
     4561 cggtcgtcgt atgggagctg ccaaaggcgg ctacagtctg gaagaaggcg aattcggtct
     4621 gaaggcgctg caggatggct ggattacctc ccgtcagatt gaagcagcgc gtgtcgccat
     4681 caatcgccat ttacggcgtg gcggtaaagt gttcatccgt gtctttccgg acaagcccat
     4741 tacgaagaaa cctgctgaaa cacgtatggg taaaggaaaa ggtgctccgg atcagtgggt
     4801 ggccgttgtg aaacccggac gcattatgtt tgagattgaa ggcgtacctg aaaccctcgc
     4861 ccgggaagct gtccgccttg ctggatttaa acttcccatt aagacccgct ttgtatcgcg
     4921 tatgcaatag tggctggtcc caactacgga gtgtcatgca ttgaaagcca aagaactgag
     4981 agaaaaaacc aacgaagcgc ttgagcagat gcttaaagag tgccgcgaca atatcgtcaa
     5041 tttccggttt cagcttgcga cgggtgttgt tgaaaatgtt cgtcgggcaa gagaagcacg
     5101 caaagacata gccagaatca aaaccattat aaaagagcgg gaaatggctg aggcgaaaag
     5161 gactacagaa tcatgagcac cgacgtgacg caggaaataa aagacgacaa aggcaatcgt
     5221 aaggaacgtg tgggcctcgt tgtcagtacc gctatggata aaaccattac cgtggctgtc
     5281 gatcgggtga aggagcaccg cttgtataat aaagcgctgc gccgcacaaa aaaatataaa
     5341 gcccacgatg ctgaaaatac atgtaatgtt ggtgataccg ttcgtatcag ggaaacgcgt
     5401 ccgctaagta agacaaaacg ctggcgcttg gtggaaatta ttgaacgcgc taagtagcgg
     5461 ttaaggatag attctatgat ccagatttac tctacgttaa atgttgcgga taactccggt
     5521 gcacgtaaac tgcgcgttat tcaggtgatg ggcggttcaa aaagacgtta tgccagtctc
     5581 ggcgatatcg ttaccgctag cgtccgggaa gtcatcccga acgccgctgt caagaaaggc
     5641 gatgtggtca aggccgttat tgtccgcgtg aaacaggaca cacagcgtcc ggacggaaca
     5701 gtgattcgtt ttgattcaaa tgctgctgtt ctgatcaatg cacagaaaga gccgcgcggc
     5761 acccgtattt tcgggccggt gcctcgcgaa cttcgtgaag gcggtttcct gcgtattctc
     5821 tctctggccc ccgaagtatt ataggattag gtgtaccatg tatatcagaa aaaaagatat
     5881 cgttgttgtt acaagcggtc ggtataaagg acggcgcggg cgggttcttg aagtttaccc
     5941 caagaaagat acgctccttg tcgaaggcgt taatatccgc aagcatcata ccaaacccgg
     6001 ccggggaaac cagaccggcg gaatcgttga acgggaagct cccattcaca tttccaatgt
     6061 aatgggctgg tgcgaggaaa aacagcagcc gtcgcaaatt cagatgaaaa aacttgccga
     6121 cggcaggcgg gtccgcgtgt ggaaacttac cggagaagta ttggattaac tctacaggac
     6181 agcgcagcct gtctggtact cagtgaagca gcaggatctg ccaggagaaa taagtcgtgg
     6241 ttgcaagatt aaaagagcac tacagaaaag aaatcgtggg cgccctcacc aatgagttca
     6301 actataaaaa tgttatggaa gtaccttgcc tggtaaaggt ggtggtgaac atgggcgtgg
     6361 gcgatgccat tgctgatgcc aagaatatgg acgccgccat tgcggaactg gcgcagatta
     6421 caggacaaaa agccgtgatc cgcaaagccc gaaaatctat atccaatttc aaactccgtg
     6481 caggtgctaa tattgggtgc atggtcacct tacgcggtga ttatatgtgg gagtttgttg
     6541 atcggttgtt taatattgcg ataccccgga tccgcgactt ccgcggactg ccgcctaaag
     6601 cgtttgataa acagggcaat tataccctgg gtattcgcga gcagacgatc tttccggaaa
     6661 ttaatctgga taaagtaagc cgggttagag gaatgaacgt gacatttgtc atcaaaaact
     6721 ctaattccgc tgaagaaagt tatgcattgt taaaacatct cggaatgccc tttgccgggt
     6781 aaattgatga caggtagcat cagggcgttg aacgctcaac agaaggttta cagcgttggc
     6841 taaattaagc aagaaagaaa agcacaagag gcagttgaaa cttgttgaaa aatataagga
     6901 aaaacgcgcc tccttgaaag caacgatgat tgatcccgaa ctctctctgg aagagagact
     6961 tgcagcaggt cgtgctctgg cgaagcttcc cagggattcc agcccggtgc gactgcgcaa
     7021 ccgctgcaaa ctgagtggaa ggcccagagg gtacttgaga aaattcggtc tttccagaat
     7081 cgcgctgcgc gaactcgcgc acgaaggcaa aattcccggt gtcaccaagt caagttggta
     7141 aaattatttc ttgaacaagg attgaaaagt tatgtcaatg agcgacccta ttgccgattt
     7201 gctgacccgg attcgtaatg gtcttcaggc acagaagcca agcgttgatg ttccgcgctc
     7261 ccaaatgaaa gttgccattt gtcgtgtgct gaaggaacag ggatatattg cgggttttga
     7321 agaactcgat aatactccgc agggaaccct tcggattgca ttgaaatatc tgcccgatcg
     7381 ccggcccgtt ttacagggtg ttcgcagaat cagcaagcct agccttcgcg tttacatggc
     7441 ccatagcgaa ctgaaacctg ttcgcagtgg tctgggcatt tctattgtca gtacttccaa
     7501 aggtgttatg acaggaaaac aagcccgtgc ggaaaatgta ggcggtgaac tgatttgtga
     7561 agtatggtaa acaaactgac cgcccaactt gattttaata acataggagt tacagcgccg
     7621 tgtcccgtat aggaaaatta ccggtacccg tacccgcagg tgtgacctgc acgctcaacg
     7681 ggtctcattt gaaagtcacc gggcccaaag gctctctgga agccagcttt tcaccggaga
     7741 tgacgatgac cctggctgat ggacagttga atgtcatgcg tccaagcgat gccaaagaac
     7801 ataaagcgct ccatggtcta acccggacgt tggcgaacaa tatggtcgta ggcgtaagcg
     7861 aaggctttaa aaagacgctg cttattgaag gcaccggcta tcgtgcttcg ctgcaaggta
     7921 aaaatcttaa ccttgtcatc gggtacagcc accccgtagt tgttgaaccg cctgaaggca
     7981 ttacgctgga agtggaaggt acgagcacca ttcatgtgac gggcatcaac aaagagctgg
     8041 tcggtcaggt tgcggctaat attcgcaaga tccgcgctgt tgagccgtac aaaggcaaag
     8101 ggatccggta tcagaatgag tttgtagtcc gcaaagaaag caagactaca ggaaagtaag
     8161 tactaatcag ccagagccgg cagccgaaaa agccggttta ttgaaaggca tatcatggct
     8221 catacgaacc ctaaaatcat acagcaaaaa ctgcgtgcgg aacgcattcg caagaaaatc
     8281 agcgggactc ctgagcggcc ccgtctgcat gtcgggcgca gcctgaagca tatttatgct
     8341 cagttaattg atgataccac agggcgcact ttagccactg cctcttccct ggcgttgaaa
     8401 atagacggcg gcaatattca gggtgccaaa gaagtgggaa aatccatcgg tgaaaaggca
     8461 aaagccatag aagtgaccca ggtcagtttt gacagaggcg gtcatttgta tcatggcaga
     8521 gtcaaggcgc ttgctgatgc ggcgcgtgaa gcgggactgc agttttagca gacaggttac
     8581 aagtcaagat agttttaaac acatgaggcg gccagatgaa tcgcaaagct gagttaacca
     8641 acgaatttaa agaagatgat tcaaacgtac agcacgttgt gaaaatttac cgtgttgcta
     8701 aagtcgtaaa aggcggcagg cgctttaatt tcagtgcgct ggtcgttgta ggcgatcgga
     8761 acggtaatgt cggagctgca ctgggtaaag cggctgaagt tcccgaagct attcgtaaag
     8821 ggactgaaaa agcctggaaa aacctgatca atgtgcccat tgtcgccacc acaattcctc
     8881 atgaaatcac tgggcgtctt ggggctgcac gggtcttatt aaagcctgcc tcgcgtggta
     8941 ccggtgtggt tgccggtggt gcggttcgtg ctgttgtaga tgctgcgggc tatcagaata
     9001 ttctgactaa gtcgcttggt tccgactgtg tgaccaacat cgtctgggca accct
//
LOCUS       DAOPWB010000123         9018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7730_length_9018_cov_4.553609, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000123 DAOPWB010000000
VERSION     DAOPWB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7730_length_9018_cov_4.553609"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..2562
                     /locus_tag="PKN92_10440"
     CDS             553..2562
                     /locus_tag="PKN92_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ49254.1"
                     /translation="MPVFSDALLAALNAPQREAVITTEGPLLVLAGAGSGKTRVITTR
                     IAYLIEKQAAAPESILAVTFTNKAAEEMRVRVGELVGQEKAKKVTISTFHSFCLQVLR
                     RDIGHLGRRRNFTIYTENDARILLRRVTDELGTEESERYSPARFHQAISLRKNHGGKA
                     PAISRAVRISAADEKYESRLSHVYESYQSALQAANSLDFDDLLLFTHELWQRFPEVLE
                     SVQRQFRYVMVDEYQDTNRLQYELLRMLTAAHRNLCVVGDDDQSIYSWRGAAPRNILD
                     FEKDFSEARVIALEQNYRSTGVILAAANGVIGNNRERREKKLWTSRGAGRLCDLFIVG
                     DEEEEAREARRWLEHIVRSTKAAWRDFAVLYRSNMQSRPFELAFREAGIPYKVFGGQD
                     FFERAEVKDLISYLKLLANPRDEAAFLRIVNMPRRGIGDSVLHQAHEVCRTEGCSLSK
                     ALFVLLERGQTPSRSEGGIRRFLYMLQDFRKRYKENGHGLRETTEALIRAIGYAEEVE
                     RVSKTPEQAQARLQNLDVVLQALSRYVEEAACPSLSGFLDQTHLDNASMEERNHYETD
                     DQVSLMTIHSAKGLEFPFVFIVGLEDGCLPHEQSMKEGGIEEERRLFYVALTRGQRHV
                     TLFQALSRMRHGKERECKPSRFLTEIPAELLRMHGCAAPQMMSRG"
     gene            2697..4028
                     /gene="miaB"
                     /locus_tag="PKN92_10445"
     CDS             2697..4028
                     /gene="miaB"
                     /locus_tag="PKN92_10445"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNZ49255.1"
                     /translation="MTRKAYIKTYGCQMNEHDTQRMHDMLKEMGYVPAETADEASVIL
                     VNTCSVRANPENKVYSFLGTLRPLKARMPDLIVGVAGCVAQQTGETLLDREPLVDFVF
                     GPDNLFRLPEMIEAAKKGERRAWTEWQSWEGPVKEFVPDTWVEQGHVEGSKAYITITK
                     GCNNHCAYCIVPHTRGREVSRSPESILREAHGMIDRGARELWLLGQNVNSYTGGPEYG
                     FYELLDEVSRLDGLLRLRFTSPHPRDWNNRLADLMAERPTICKHLHLPFQAGSDAVLK
                     AMRRRHTLEEYLDKVRYLREKIPAVEISTDLIVGFPGEKEEDFERTLYTLREVRFSQV
                     FPFKYSTRPGTRAASLPDDVSRKEKEDRLARVIALQDKINEEAQEALVGTVQQVLIDG
                     PHPRIPGRWNGRTDGYRSITVVADGLNPGELVDAKVTAARGHWLQADLHRP"
     gene            4077..5615
                     /locus_tag="PKN92_10450"
     CDS             4077..5615
                     /locus_tag="PKN92_10450"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNZ49256.1"
                     /translation="MKLSMDMVTSISVCTAEDMNWEGGGNCLVVPLYRKEFPLNSALL
                     QEEEELVLQALVDREVLTGAAGTCYYLPAPSRPYSGVLVLGLGDRKKATAESLRRAVG
                     KAASLLKANRVERIGVDLVHFPELPVPAFVESLNLAQYRFDTYKKEEEKPAPAVREVV
                     LVVADSVDKAALLEAAERAAFFSVAANGARHLANTASNDMTPAVLAAAAQKIAEEGGE
                     QCTCAVLDQQRMEELGMNALLGVARGSGKPPQLICLEYTHPEATRTITLVGKGVTFDT
                     GGISLKPGAKMHEMKFDMCGAAVVLATMMAVTRLKPVLNVISVVPAVENMPDGNAQTP
                     GEIVRACNGKTIEVLNTDAEGRLILADALAYAVDTWKPDCIVDLAALTGAVVIALGHV
                     AAGVVGSDSALVQSLVAAGESTGERLWELPLWEDYEEMMEGTFADLSNIGPEREAGTI
                     TGAAFLKAFTGDTPWAHIDIAGVSYGIRNKPYLKPEYATGFGVRLLLEWLTGRTAQSG
                     QASE"
     gene            5619..6944
                     /gene="mtaB"
                     /locus_tag="PKN92_10455"
     CDS             5619..6944
                     /gene="mtaB"
                     /locus_tag="PKN92_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNZ49257.1"
                     /translation="MQPPADNAEKVNEARTASIHTLGCRLNQAETRLLEEQLREAGYR
                     IVPFDQPADVGIVHGCVVTVEAEAKTRKYIQRFRRKNPGSITVAIGCFAQTAADSAAV
                     LGVDLVLGNASKMELLHHLEAMEDSKGAVVTPRPKRDAFMLPFQKSSDPVDRRINLKI
                     QDGCDEMCSYCFIPFARGRSRSRYLSDVLDEAASLVERGARELILSGVNVGDYREGGA
                     DLLTLIDRLNGLRPRPRIRISSIELNTIPEGVLERMADTDHGLLPYLHIPLQSGSDPI
                     LKAMGRPYTADAYLRFLEKARNEVPMIGLGADVMVGFPGETEALFEETRRLIDASPLD
                     YLHVFPYSERDAVASSRLSHKISDQDKRRRSEIVRALGDQKQEQGQLRFLHRRCRVLF
                     EEKRGAYWIGSNEEYFEAAVQSEECLKNRVASVDIREVREERLFGTLAV"
     gene            7005..7793
                     /gene="srlD"
                     /locus_tag="PKN92_10460"
     CDS             7005..7793
                     /gene="srlD"
                     /locus_tag="PKN92_10460"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HNZ49258.1"
                     /translation="MNTLTGKKAIVTGGAQGLGAAISERLAAEGCDLEIWDINEEVAS
                     SSAAAIASKTGQQVRYALVDVTDAGAVRAAVDHAVSWMGGLDIMVCNAGILISGDSIE
                     MDASAWRKVIDVDLTGYFFCAREAARVMVPARKGSIIQINSKSGKKGSFKNAAYAAAK
                     FGGVGLTQSLALEFAESGVRVNAILPGNLLNSPLWVNSLFKQYARNQGISEAEVRQKY
                     IDQVPMKRPCEYEDVCNVVVFFAGEGSSYMTGQAINVTGGQEMR"
     gene            complement(7802..8482)
                     /locus_tag="PKN92_10465"
     CDS             complement(7802..8482)
                     /locus_tag="PKN92_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNZ49259.1"
                     /translation="MSETVSIILLQYLCLLFSLSVHEGAHAAMANYWGDPTARLMGRM
                     SLNPIKHADIMGTVILPLFAMITGWRFMFGWAKPVPYNPRNLRDRKKGPVFIALAGPG
                     SNLLVVFAAVAVLRGLALLPESDTATLFMRALFYLVMINSLLLLFNLIPIPPLDGHHV
                     VEFFLPYEWKKKFEQIGPYSIFILFFLIFYLNILRVPMAFVLRGTLTLAFYGTPVWGE
                     RILSIIGG"
     repeat_region   8664..9017
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8664..8687
                     /rpt_unit_seq="gctgtagttggtagggaatccccg"
BASE COUNT         2088 a         2262 c         2659 g         2009 t
ORIGIN      
        1 acaacgcact gttcaccagg aaaactaatt tacagaaact tcttgacata accttttgtt
       61 aaaagaaact ccattgcttt actccttgtt ctgccattaa accatgactg tttcagcgtt
      121 atattgcttg cgcaaacaga agcaaccctc acgttgcagg ctgctgttac gatgcaaatt
      181 ccatcttcct gctaattaac ctataggaga accattagat tatagcacgg gtatgacaaa
      241 aatacaagag tttcgagaaa aattcaatta agaaaccgct ttaatgcttt tgtatatatt
      301 ataattcgta ttatatggag gtgcaatgta agaaaagagt ggtttgataa cttcaaaaat
      361 cggtttcgac aaggtacgct ttgtgtgaat aaagtcatgc ggcgcaagcc agtgaaaggt
      421 tttttccact actaccatca cctgcgctct ctgctgaaaa tcgtactttt attgagttga
      481 ttttttctgc cggatcatgc acaatgcgcc tttctgctgt ttcccctgcc atccgattcg
      541 atgaggtgtt tcatgcctgt tttttccgat gctttacttg ccgctctcaa tgcgcctcag
      601 cgtgaggcgg tgataacgac agaggggcct ttgttggtgc ttgcgggggc gggcagcggt
      661 aaaacccgtg tgattaccac gcggatcgcc tacctgattg aaaaacaggc cgccgccccg
      721 gagtccattc ttgccgtaac cttcacgaac aaagcagcgg aagagatgcg tgtccgtgtc
      781 ggtgaactgg tggggcagga aaaggcgaaa aaagtgacca tctccacttt ccactctttt
      841 tgcctgcagg tgcttcgccg ggatatcgga catctgggcc gccgccggaa tttcaccata
      901 tatacggaaa atgacgcacg tattctcctg cgccgtgtta ccgatgaact gggaacagaa
      961 gaaagcgaac ggtatagccc cgcccgtttt catcaggcca tcagcctgcg caagaatcat
     1021 ggcgggaagg cgccggcgat aagcagggca gtccggattt cggcggcaga cgagaaatat
     1081 gagtcccgcc tgtctcacgt ttacgagagc taccagtcgg cactgcaggc ggcgaacagt
     1141 ctggacttcg acgatctgct gttgttcacc catgagttgt ggcagcgttt tccggaagtg
     1201 ctggaatcgg tacagcgtca gttccgctat gtgatggtgg atgagtacca ggacacgaac
     1261 cggcttcagt atgagctttt gcgtatgctg acagcggcgc atcgcaatct ttgtgtagtg
     1321 ggggatgatg atcagagtat ctacagctgg cgcggtgcgg ctccgcgtaa tattctggac
     1381 tttgaaaaag atttttctga agcgcgtgtt atcgctttgg aacagaacta ccgttcaacg
     1441 ggcgtgattc ttgcagcagc aaacggggta atcggcaaca atcgtgaacg gcgtgagaaa
     1501 aaactgtgga cttcccgggg tgccgggcgt ttgtgcgatc tttttattgt cggcgacgag
     1561 gaagaggagg ctcgcgaggc gcgtcgctgg cttgaacaca tcgtacgaag caccaaagcg
     1621 gcgtggcgcg attttgctgt gctgtaccgc tcgaacatgc agtcccgtcc ttttgaactg
     1681 gctttccgtg aagcgggcat accctataaa gttttcgggg gtcaggattt ttttgagcgt
     1741 gcagaagtca aggacttgat cagttatctg aaattgctgg cgaatccgcg ggatgaggcg
     1801 gcttttctgc gcattgtgaa catgccccgg cgcgggatcg gcgattcggt actgcatcag
     1861 gcacacgaag tctgccgtac ggagggctgc tctctgtcaa aggcattgtt tgtactgctg
     1921 gagcgcggtc agacgccatc gcggagcgaa gggggcatcc gccggttttt gtatatgctg
     1981 caggatttcc gcaagcgata taaagaaaac gggcacgggc tccgggagac cacagaggcg
     2041 ctcattcgtg ccatcggcta tgccgaagag gtggaacggg tcagcaaaac gccggaacag
     2101 gcacaggcac gcctgcagaa tctggatgtt gtgcttcagg cgctgtcccg ttatgtggaa
     2161 gaggcagcgt gtccgagttt atccgggttt cttgatcaga cccatctgga taacgcttca
     2221 atggaggaac ggaaccatta tgagacggat gaccaggtga gcctgatgac gattcacagc
     2281 gcaaaagggc tggaatttcc ttttgtgttt attgtcggac tggaagacgg ctgcctgccc
     2341 catgaacaga gcatgaaaga gggtggtatt gaagaggagc ggcggctgtt ttatgtggca
     2401 ctgacacggg ggcagcgcca tgtgaccctg tttcaggcgt tgtcccggat gcgccatggt
     2461 aaagaacggg agtgcaagcc gagccgtttt ctgacagaaa ttccggcgga actgcttcgg
     2521 atgcatggct gtgccgcccc gcagatgatg agccggggct gagaggcgag ggcagggcat
     2581 cgcgtgctct gcagcaaaaa gagagtgata taacggggga atgatggtat aatttccctt
     2641 tgatcccctg tctttgtgac agaattgcca ttgaccaacc cgccggatgg atatttatga
     2701 cccggaaagc ctatatcaaa acctacggct gccagatgaa cgagcacgat acgcagcgga
     2761 tgcatgacat gctcaaagag atgggctatg tcccggcgga gactgccgac gaagcctccg
     2821 ttattctggt caatacctgt tcggtgcgcg ccaatccgga aaacaaggtc tacagttttc
     2881 tcggaacgct ccgtccgctg aaagcccgga tgcccgatct gattgtgggg gtggcgggct
     2941 gtgtcgcaca gcagaccggc gaaacgcttc tggatcggga gccgctggtg gattttgttt
     3001 tcggccctga caacctgttt cgcctgccgg aaatgattga agcggcgaaa aagggcgagc
     3061 ggcgcgcctg gaccgaatgg cagtcctggg aaggacctgt caaggaattt gtgcctgata
     3121 cctgggtgga gcagggacat gtggaaggct ccaaagccta tatcacgatt accaaaggct
     3181 gcaataacca ttgcgcctac tgtattgtgc cccatacccg gggcagagaa gtgtcccgtt
     3241 cgccggaaag tattctccgg gaagcacacg gtatgattga ccggggtgcc cgggaactct
     3301 ggcttctcgg tcagaacgtg aattcctata caggcgggcc ggagtacggt ttttatgagc
     3361 tgctcgatga agtatcccgc cttgacgggc tgctgcggct tcgtttcaca tcgccccatc
     3421 cccgggactg gaataaccgc ctcgctgacc tgatggcgga gcgcccgacg atttgcaagc
     3481 atctgcatct gccttttcag gccggttccg acgcggtttt aaaagccatg cgccgccgcc
     3541 acaccctgga agagtatctg gataaggtgc gttacctccg tgagaagatt cccgctgtgg
     3601 aaatcagcac agacctgatt gtcggctttc ccggcgaaaa ggaagaagat ttcgaacgta
     3661 ctctctacac gctccgggaa gtccggttca gccaggtgtt tccgttcaag tattccaccc
     3721 gtcccggcac ccgggcggcg tctcttcccg atgatgtgtc ccgtaaggaa aaagaggatc
     3781 ggcttgcacg ggtcatcgcc ctgcaggata aaattaacga agaggcgcag gaggcgctgg
     3841 tgggtaccgt acagcaagtg cttatcgacg ggccccatcc ccgcattccc ggccggtgga
     3901 acgggcgtac cgacgggtac cgttccatta ctgtggtggc ggatgggctg aatccgggtg
     3961 aactggtgga tgcgaaggtg acagccgccc gggggcattg gcttcaggcg gatctgcacc
     4021 gcccctgaca gaatgaaaca aacagttcag aaaaatacga aaagaaggaa tcaattatga
     4081 aactatcaat ggatatggtc accagtattt cggtctgcac tgccgaagat atgaactggg
     4141 aagggggcgg aaactgcctg gtggttcccc tgtatcgcaa agaatttccg cttaacagcg
     4201 cattgcttca ggaagaagag gaattggttc ttcaggcgct ggtcgaccgg gaagtcctga
     4261 ccggtgccgc cggaacctgt tattatctgc cggcgccgtc ccgtccttac agcggtgttc
     4321 tggtactggg gctgggcgat cgcaaaaaag caactgccga aagtttgcgc cgcgccgtag
     4381 gtaaagcggc ctctctgctt aaagccaatc gtgtggaacg catcggcgtt gatctggttc
     4441 attttcctga actcccggtg cctgcctttg tcgagagcct gaatcttgcg cagtatcgtt
     4501 ttgacaccta taaaaaggaa gaagaaaaac ccgctcctgc tgtccgtgaa gtcgtgctgg
     4561 ttgtggcgga tagtgttgat aaagcagcac tgcttgaagc cgctgagcgc gccgccttct
     4621 tttctgttgc cgccaacggt gcccgccatc ttgccaatac ggcatccaat gatatgactc
     4681 ctgccgtgct tgcagccgca gcgcagaaaa tcgccgaaga gggcggggag cagtgcacct
     4741 gtgccgtcct cgatcagcag cgtatggaag aattgggcat gaatgcactg ctcggtgttg
     4801 cccggggctc cggaaagcca ccgcagctta tctgtctgga atatactcat cccgaggcga
     4861 cccggacgat taccctggtc ggcaagggag tcacctttga cacgggcggt atttctctga
     4921 agcccggcgc aaaaatgcat gagatgaaat ttgatatgtg cggtgcggct gtcgtgcttg
     4981 ccaccatgat ggctgtgacc cgccttaaac cggtgctgaa tgtgatcagc gtggttcctg
     5041 ctgtggaaaa tatgcctgac ggcaacgccc agacgccggg tgaaattgtc cgtgcgtgca
     5101 acggcaaaac catagaagtg ctgaatacgg atgcggaggg acgtctcatt ctcgccgatg
     5161 cgctggccta tgcggtggat acctggaaac ccgactgtat agttgacctt gccgcgctga
     5221 ccggcgctgt cgtgattgcg ctgggacatg tggcggcggg cgtggtgggc tcggatagtg
     5281 cgctggtgca gagcctcgtt gctgcaggcg aaagcacagg cgaacggctc tgggagctgc
     5341 cgctctggga agattatgag gagatgatgg aaggtacttt cgccgatctc agcaatatcg
     5401 gacccgaacg ggaagcaggc accattaccg gcgccgcttt tctgaaggcc ttcaccggcg
     5461 ataccccctg ggcgcatatt gatattgccg gtgtgtccta cggtatcaga aacaagccct
     5521 atcttaagcc ggaatatgct accgggttcg gtgtgcggct tcttctggaa tggctgacag
     5581 gcagaaccgc acagagcgga caggcatcgg aatgacacat gcagcctcct gctgacaatg
     5641 ccgaaaaagt gaatgaggca cgcactgcct ccattcacac attgggctgc aggctgaatc
     5701 aggcggaaac acgtctcctg gaagaacagc tgcgcgaggc gggataccgt atcgtgcctt
     5761 ttgatcagcc tgcggacgtg ggtatcgtgc atggctgcgt tgttacggtg gaggcggagg
     5821 caaaaacacg caaatatatt cagcgtttcc ggaggaaaaa cccgggcagc attactgttg
     5881 ccatcggatg ttttgcccag acagccgccg attccgccgc agtgctgggt gtggatcttg
     5941 tgctcggcaa tgcatcgaaa atggagttgc tccaccatct ggaggcgatg gaagacagta
     6001 aaggcgctgt tgtaaccccg cggccgaagc gggacgcttt tatgcttcct tttcagaaga
     6061 gcagtgaccc tgttgaccgg cgcatcaatt taaaaataca ggacggctgc gacgagatgt
     6121 gcagctactg ttttatccct tttgcccggg gcagatcccg gtcgcgctat ctcagcgacg
     6181 ttctggacga ggcggcatca ctggtggagc ggggtgcccg ggaactgatt ctgtccggtg
     6241 tgaacgtcgg cgattacaga gaaggcgggg cggatctgct gaccctgatc gaccggctga
     6301 acggactccg gccccggccc cggatccgca tcagttccat tgaactgaac acaatccccg
     6361 aaggggtgct ggagcgtatg gccgataccg atcacgggct cctgccttat ctgcatatcc
     6421 cgctccagtc cggaagtgac cccattctga aagccatggg gcggccttat acggcagatg
     6481 cctatctgcg ctttctcgaa aaagcacgaa atgaagtgcc catgatcggt ctgggtgccg
     6541 atgtcatggt gggctttccc ggagaaacag aagcgctgtt tgaagagacc cggcgattaa
     6601 ttgacgcaag tcccctcgat tacctgcatg tctttcccta cagcgaaagg gatgcggtgg
     6661 catcgtcacg gctcagccat aaaatcagcg atcaggataa acgccgacgc agtgagatag
     6721 tgcgggcact tggcgatcaa aagcaggaac aggggcagct gcgttttctt cacagacgct
     6781 gtcgtgtcct ctttgaggaa aagcggggcg catactggat cggcagcaac gaggagtatt
     6841 ttgaagccgc cgttcagtcg gaggaatgcc tgaaaaaccg ggtcgcttcc gtagatatcc
     6901 gggaagtccg ggaagaacgg cttttcggta cgcttgctgt ataataggcg gcggcagagc
     6961 actgtatacc gggaaaagaa acccgaaaac aaaaggaatt cggcatgaat acgcttacag
     7021 ggaaaaaagc cattgtaacc ggtggcgctc agggactggg tgcagctatt tcggagcgtc
     7081 tggcggcgga aggctgtgat cttgaaattt gggatatcaa tgaggaagtc gcctcgtcat
     7141 cagcagcagc cattgcgtca aaaacagggc agcaggtacg ctacgccctg gtggatgtga
     7201 ccgatgccgg agcagtgcgt gctgctgtcg atcatgccgt gtcctggatg ggcggtctcg
     7261 atatcatggt ctgcaatgcg ggcattctta tctccggtga ttccattgag atggacgcat
     7321 ccgcctggcg caaagtcatt gatgtggatt tgacaggtta ttttttctgc gcccgtgaag
     7381 cggcccgggt gatggtgccc gcccgcaagg gcagcatcat tcagatcaat tccaaatccg
     7441 gtaaaaaagg gagcttcaaa aatgccgcct atgccgccgc caaattcggc ggtgtgggac
     7501 tgacccagag tctcgcactc gagtttgccg aatccggagt gcgcgtgaat gccatccttc
     7561 ccggcaacct cctgaattca ccgctctggg tcaacagcct cttcaaacag tatgcacgga
     7621 atcagggaat cagcgaagcg gaggtgcgcc agaaatacat cgatcaggtg cccatgaagc
     7681 gcccctgcga atatgaggat gtgtgtaatg tggtcgtctt tttcgcaggg gaaggctcca
     7741 gttacatgac cggtcaggcg ataaatgtga cgggcggcca ggaaatgcgc tgagtctctt
     7801 tttatccgcc gatgatgctg agaatccgct cgccccagac gggggttccg tagaaggcga
     7861 gagtcagtgt gccccgcagc acgaaagcca tggggacgcg caggatgttc aggtagaaga
     7921 tcaggaaaaa tagaatgaaa atgctgtagg ggccgatctg ctcaaatttc ttcttccatt
     7981 catagggcag aaaaaactca accacatgat gcccgtccag aggcggaatg ggaatcagat
     8041 taaagagaag cagcagtgag ttgatcatga ccagataaaa gagcgcgcgc atgaacaaag
     8101 tggctgtatc cgattcgggg agcagcgcaa gaccgcgcag caccgcaaca gcggcaaaaa
     8161 ccaccagcag gttggaaccg gggccggcaa gggctatgaa aacaggccct tttttccggt
     8221 cgcggagatt gcgcgggtta tagggaaccg gctttgccca gccgaacatg aatcgccagc
     8281 ccgtgatcat ggcgaagagc ggaagaatca ctgtgcccat gatatcggca tgttttatgg
     8341 gattgagaga catgcggccc atgagacggg cggtcggatc gccccagtaa ttggccatgg
     8401 cagcatgagc cccttcgtgc acactcaatg aaaagagaag gcacaaatac tgcagaagaa
     8461 taatgcttac cgtttccgac actgtgttgt cctttgaatg tggctgtccg gccggagatg
     8521 cgcctgaatg aaaagcaatg ccccgctgga atactactat tctaccctac ctttcgtgtc
     8581 tgatcaaagc ggagtcattc tttgtccatc tgcagataag atctttttta tgcgcccgga
     8641 gtttcgctta gatttcagat tgtgctgtag ttggtaggga atccccgctc aggtatactg
     8701 gatctccgga ggaggcggtg tcgacgagcg ctgtagttgg tagggaatcc ccgctcaggt
     8761 atactcaaca acaacaacga ccagcgacaa caacagctgt agttggtagg gaatccccgc
     8821 tcaggtatac tggcggacga ccttgctgat atgcgcgatt ggctgtagtt ggtagggaat
     8881 ccccgctcag gtatactaac tagaacatag agtgaaacag ctgattagct gtagttggta
     8941 gggaatcccc gctcaggtat actttcacgc gcctaattcc cagtggttct taggctgtag
     9001 ttggtaggga atccccgc
//
LOCUS       DAOPWB010000124         8993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7768_length_8993_cov_5.031774, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000124 DAOPWB010000000
VERSION     DAOPWB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7768_length_8993_cov_5.031774"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2142
                     /locus_tag="PKN92_10470"
     CDS             <1..2142
                     /locus_tag="PKN92_10470"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNZ49260.1"
                     /translation="AAATVFLSNTAFMAFELGVSRLIAREFGSSLYTWTMVIGLVLAG
                     ITFGNSLGGRLADRSFSNRTVAAVFSLSALAVMLSPVWNGLVRTQRSSVLFLALLSWP
                     MQIGLQVAALCFIPCLFIGMISPLLVRRALDKGMGAGASVGMIYAWGSLGGIFGTFLS
                     GYWLIQWMGALPMTLTVALSLAVAGVLYKPRHFLPLTAMLLSALFLGASFFPRQAVFA
                     PVYAWIRTHSDYANRTIYEDESQYSYIAILKDEENPDLREMLLDQLVHSCIDMTDPTH
                     LLYEYEWVYDSVLEKRHPRPQPLRTLVIGGGGYAYPHHLEKVRPGSSILVSEIDPAVT
                     RAAHAALGLPENTQIDIRDMDARNVVDDLLAQKEANPSEYEGFDYIFGDSINDYTVPY
                     HLTTLEFNRRLHSLLKEDGMYLFNMIDLLDSGAFLSAVVHTCRQVFADVAVFNTGRAS
                     FVRDTFVVVCAKQPVPLDDIPARIAAAHAYIGTRIPNVTLNALIERNGSLLLTDNFAP
                     VENLLAPVVRTRAGDPGELYLAFARRDNASGNTERAMKRCMKALSIHPQWPEAREFLA
                     QLRKQSGDREGTIEALAEAVNGHAQPAVAWSNLAAALLEAQRTEEAMAAWQESVRIRP
                     DYVFAWYNLGVLYGMRQEIPEAIACWKKAVETNPGHGDSLYNLAAASLMLGDPASAQK
                     YVEQMKAHHLPVDAQLLAAIQQQSGATPSEK"
     gene            complement(2149..3228)
                     /locus_tag="PKN92_10475"
     CDS             complement(2149..3228)
                     /locus_tag="PKN92_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49261.1"
                     /translation="MTDQDEERRDQDPTRLCHTCRMPVSALATRCRFCGAKLERPRKS
                     DEVLTVKDLGGERGSTYTPSANVVGALEAFAEEEKAQSSNQTQTGGTRSRIRRIKRTQ
                     EESRQKLEEALDELDPSKIDIYAKDILSRKPKESEKKPAGTLDDLGRKLFSAVIFIVA
                     VVFLYFIVTFGWNWLRVGGAVQPAAFQTFPNRAETMLAEGQPLPDVHREAARACQVDN
                     SPENSRILSRVRRRLMEQIEAQAYSKPFNMQNITDANRDSSAAATVDFDQSLADLAER
                     MRREAATYKFILTSLNKEAETALFTLNNPYVSEKEQTVSPGELLLDRFLVTSISSRGV
                     ALEDTHPKAGRRHLLAKPLTTVLPD"
     gene            complement(3377..4114)
                     /gene="rlmB"
                     /locus_tag="PKN92_10480"
     CDS             complement(3377..4114)
                     /gene="rlmB"
                     /locus_tag="PKN92_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNZ49262.1"
                     /translation="MPLNPLEIVPGRLPALACLHAKKRTVKKLYLYGKSQTAEEFERL
                     LPQEKIVYADRHQLDQLAGGVLHQGVLLHAAPLPVLDLDDWLVRHDRPDCILLALDCI
                     EDPQNFGAMVRSAAACGASGVLFQKDRAAPVSPAAVKAAAGGMEYVDLIQVTNLARSL
                     GALQEAGFWTAALDGRADTTLWSSDLTGRITLVIGNEGAGVRRLVREKCDYRLAIPLP
                     GEVASLNASVAAGIALAECMRQRAAKD"
     gene            complement(4114..4551)
                     /locus_tag="PKN92_10485"
     CDS             complement(4114..4551)
                     /locus_tag="PKN92_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49263.1"
                     /translation="MDLTFLKWPIIILVIAGIAFLFTSPGVNFLVNHYTKATVGADFA
                     KDLSDESGLTRIGGYLLFTWQYQKAYDTMNLAVARYGPEGRHYYYNLYRMARCLDRLE
                     RYRESYNILMDLISANAHDADNRVADNDNLRLRASKLKEVHGL"
     gene            complement(4566..4919)
                     /locus_tag="PKN92_10490"
     CDS             complement(4566..4919)
                     /locus_tag="PKN92_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HNZ49264.1"
                     /translation="MAAPKIVGCFNHPGKEAMGRCRQCSKPICKECGFVTPAGMYCSN
                     ECRDKHEDFMKRATDVDLSKRQKRSLRARLGSVFSLIVVLVVLFFGAVLLGWLVHIPY
                     LSSFSITVRSFLGLM"
     gene            complement(4992..6131)
                     /gene="mnmA"
                     /locus_tag="PKN92_10495"
     CDS             complement(4992..6131)
                     /gene="mnmA"
                     /locus_tag="PKN92_10495"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNZ49265.1"
                     /translation="MISTEENRMPPWGARVMIAMSGGVDSAAVAVLLAEAGYDCLGAT
                     LRLTGDKEEVSVFEPCCGLQAAEDARRICTTLGIPHRTVRVVEVFEKTIIRYFAGQYA
                     EGRTPNPCIRCNRAIKFGMLFKEATRLGYDYIAFGHYARLMERNGRMTLARAAYLPKD
                     QSYVLAPLTQSQLRRSCFPLAAMTKEDARAAARRIDVVSGNKAESQEICFVPLRNYAS
                     VVEKYAGKGTPGPIRDLEGRVLGQHEGIHRYTIGQRRGLGIGAEKPLYVVWLDAAHNT
                     VWVGRDAQSLCDCFETGPLFFGGMAPQKEKFEAWVQLRYRHQPVAGVVEPRDHGARVV
                     LSEAQRAVTPGQWAVFYDGSGHILASAKIQQFERWCGGADCTFTQ"
     gene            6296..7168
                     /locus_tag="PKN92_10500"
     CDS             6296..7168
                     /locus_tag="PKN92_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ49266.1"
                     /translation="MLPLFLLMTTAFLAAGQSFDSGAPAIALYEEAMAPAHISGAMNA
                     PALHLFPLHESKKPVPAVVVCPGGGYGGLATGHEGMEVARWLNGQGIAAFICQYRVEP
                     NRHPVPLQDAQQALRVVRARAEEWNVDPVRVGILGFSAGGHLVSTVATHFDGGDPDSA
                     DPVKRQSSRPDFTVLIYPVISLLPPFGHVGSGKNLLGDNPEPGLAESLQNDQHVTKDT
                     PPAFLVASTADTGVSAENSITYYLALHRAGVPAEMHVYEPGPHGFGLGKGDPVLSTWP
                     DLFIKWLHTRAVLP"
     gene            complement(7187..8878)
                     /locus_tag="PKN92_10505"
     CDS             complement(7187..8878)
                     /locus_tag="PKN92_10505"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNZ49267.1"
                     /translation="MSAQSSEFCIESSGGVLLRRKDIYVAQLEGDYETMGRQHGELAA
                     AACGDVVALYMNGLLSKLIAQAVPSISRSAAWLIQELFRRRNSRDYGADLKAHLRGLA
                     QAYNLPLADAERLLVMPDIFHYLAGKTFTTLTSPPSCSCFFACGAATQDGKLIVGRNF
                     DFFGRGVWNDNQALIVMRPSDGQAFCWIGALGVCGSGQGFNESGLIVGLHSKFTTDLR
                     TQGAPIFKLVHEILAYCTTVEEAVARITAAPRLCGLSLFITGSRERTAAIVGYSATHH
                     ELIRPEKEYLVRTNHYVTADMQRLQRGPVAFMRHTQARFQRITALIEEKYGTLRVEDG
                     PSILSDCIDPYEDRKRLAGNLVAATNNVQSIVFSPDDDTLWLAHGDFPVCLNDRYCGF
                     SMSALLQGDEGNYEKEDLPGGSPLTGEERRGLYEFLQAWSAHLDNLDNSRAVQHLLRA
                     AEIVPGEPVFPRLAGLILLKEKKYARALPLLLKNAEYPYRDALAHAESLLWVGRCLDL
                     MGRRDEALDYYRQSSALNAQPVCAAAERNMNTAFKAHQMWSVTPEFVIGAALAKY"
BASE COUNT         1989 a         2609 c         2356 g         2039 t
ORIGIN      
        1 gcagccgcca ctgtctttct gtccaatacg gcgtttatgg cctttgaact cggcgtttcc
       61 cggctgatcg cccgggaatt cggcagttcg ctgtacacct ggaccatggt tatcggtctg
      121 gttcttgccg gtattacctt cggaaactct ctgggcggtc gtctggcaga tcgaagtttc
      181 agtaacagga cggttgcggc agtcttttct ctttccgctc tggctgtgat gctgtcccct
      241 gtctggaacg gactggtacg tacacagcgc agcagcgtgc tgtttctggc gcttctgtcc
      301 tggccgatgc agatcggtct tcaggtggcg gcgctgtgct ttatcccctg ccttttcatc
      361 ggaatgatca gccctcttct tgttcggcgt gctctggata aaggcatggg agcgggcgct
      421 tccgtcggca tgatctacgc ctggggatcg cttggcggga ttttcggcac ttttctgtcc
      481 ggctactggc tgattcaatg gatgggcgct ttacccatga ccctgaccgt ggcactgtcc
      541 ctggctgtgg caggggtact gtataagccg cgccatttcc tgcctctcac agcgatgctg
      601 ctcagtgcac tttttctggg agcctctttc tttccccggc aggcggtatt cgcacctgta
      661 tacgcctgga ttcgaaccca ctcggattat gcgaaccgca ccatctatga agatgaaagc
      721 cagtactctt atattgccat cctgaaagat gaggagaacc ctgacctccg ggaaatgctg
      781 ctggatcagc tggtgcactc ctgcattgac atgacagatc cgacccatct gctctatgaa
      841 tatgagtggg tctatgacag cgtgctggaa aaacggcacc cgcgcccgca gcccctgcgc
      901 actcttgtca tcggaggcgg cggctatgcc tatcctcacc atctggaaaa ggtgcggccg
      961 ggcagcagta ttctcgtgtc cgagattgac cccgccgtca cccgtgccgc tcatgccgcc
     1021 ttggggcttc ccgaaaacac gcaaattgat atccgggata tggatgcccg caatgtggtg
     1081 gacgatcttc tggcgcaaaa agaagcgaat ccctccgaat atgaaggatt cgactatatt
     1141 ttcggtgatt ccatcaacga ttatacggtt ccgtatcacc ttacaacgct ggagttcaac
     1201 cggcggctgc acagtcttct gaaagaggac ggcatgtacc tcttcaacat gatcgacctg
     1261 ctggactcgg gtgcctttct ctctgccgtt gtgcatacct gtcgccaggt ttttgccgac
     1321 gttgccgtat tcaacaccgg gcgtgcttct ttcgtgcggg atacttttgt cgtcgtctgc
     1381 gcaaagcagc ctgtccctct ggatgatatc cccgcccgca ttgcagcggc tcatgcctat
     1441 attggaactc gcattcccaa tgtaacgtta aatgccttaa tagaaagaaa tggctccctc
     1501 ctgctgaccg acaattttgc acctgtggaa aatctgctgg cacctgttgt gcgcacccgg
     1561 gcaggcgatc cgggagaact gtatctggct tttgcacgtc gggacaatgc ttccggcaat
     1621 acggagcggg cgatgaagcg ttgcatgaaa gcattgtcca ttcatccgca atggccggag
     1681 gcgcgtgaat ttctggcgca attgcgcaag cagtcgggcg atagagaggg tactatcgag
     1741 gcacttgcag aggctgtcaa cggccatgca cagcctgccg ttgcgtggtc gaacctggcg
     1801 gccgccctgc tggaggcaca acggacagag gaagcgatgg cggcgtggca ggaaagcgtg
     1861 cgcatcagac ctgattatgt tttcgcctgg tacaatctgg gagtgcttta tggcatgcgc
     1921 caggagatcc cggaggcaat cgcctgctgg aaaaaggctg tggaaacgaa tccgggccat
     1981 ggcgacagtc tctacaatct tgcagcggct tctctcatgc tgggagatcc tgcctctgcc
     2041 caaaagtatg tggaacagat gaaagcacat cacctgcccg tggacgcaca gctgcttgca
     2101 gcaattcagc agcagtcagg tgccacacct tccgagaagt aaggcacgtc aatccgggag
     2161 aacggtggtc agtggtttgg caagcaggtg ccgcctgcct gctttggggt gcgtatcttc
     2221 caatgcaacg ccccgggagg aaatgcttgt cacaaggaaa cgatccagaa ggagttctcc
     2281 gggcgagact gtttgctctt tctcactgac ataggggttg ttcagggtaa aaagcgctgt
     2341 ctccgcctct ttgttcagag aggtaagaat aaatttatag gtggccgcct cccgccgcat
     2401 tctctctgcc agatcagcca atgactggtc aaaatcgacg gtggcagcgg cactgctgtc
     2461 ccggttggca tcggttatat tttgcatatt aaagggtttg ctgtatgcct gcgcttcaat
     2521 ttgctccatc agtctacggc gcacccgtga gaggatacgg ctgttctcgg gcgaattgtc
     2581 cacctgacag gcccgggcag cctcccggtg cacatccggc agtggctgtc cttccgcaag
     2641 cattgtctct gcgcggttcg gaaatgtctg aaaagcagcg ggctgcacag ctccgcccac
     2701 acgcagccag ttccatccga atgtcacaat aaaatacaga aaaacaacag ccacaataaa
     2761 tatgacggca gaaaacaatt ttctgcccag atcgtccaaa gtccccgcag gttttttctc
     2821 gctttctttg ggcttgcgcg ataaaatatc tttagcgtag atgtctattt tggatggatc
     2881 taattcgtcc agcgcttcct ccagtttctg ccgcgattct tcctgcgttc ttttgatacg
     2941 ccgtattcgc gacctcgttc cgccggtctg ggtctgatta gagctctgcg ccttctcttc
     3001 ctccgcaaaa gcctcgagtg ctcccacaac attggcagag ggagtataag tgctgccccg
     3061 ctccccgccc agatccttga cagtcagcac ctcatccgac ttccgggggc gttcaagctt
     3121 ggctccgcaa aaacgacagc gtgtcgccag ggcacttacc ggcatcctgc aggtatggca
     3181 cagacgtgtc ggatcctggt ctcttctctc ttcatcctga tctgtcatta aacaatcctt
     3241 gtccaaaaat cagcaatgga acccttttca gataaacacg cattacgccc tataagaaaa
     3301 gccttttcat tatacaaatc atggacaaaa acagcaagcc ctgctaaagg aacatgcgct
     3361 ttgcaagtga aaaacatcaa tctttagcgg cacgctggcg catgcattcc gccagcgcaa
     3421 tccctgcagc aacagacgca ttcaggcttg ccacttcccc gggcagcggg atcgccagac
     3481 gataatcgca tttctcacgg accaggcgtc ggacaccggc accttcattg ccgatgacca
     3541 gcgtgattct tccggtaaga tcggaagacc acagcgttgt gtccgcacgg ccatccaatg
     3601 ctgctgtcca gaatccggct tcctgcaacg ctccaaggga acgcgccaga ttggtgacct
     3661 ggatcaggtc aacgtattcc atgccaccgg cagccgcttt gaccgccgcc ggactgaccg
     3721 gagcggcacg gtccttctga aaaaggacac cggaggcacc gcatgcggca gcactgcgta
     3781 ccatggcgcc gaaattctga ggatcttcga tgcaatcgag agcaaggaga atgcagtcag
     3841 ggcgatcgtg ccggacaagc cagtcatcaa gatccagaac gggcaaaggt gcggcatgca
     3901 gcagaacacc ctgatgcagc acaccgcccg ccagctgatc cagctgatgc ctgtcggcat
     3961 atacgatctt ctcctgcggc aaaagacgct caaactcctc cgccgtctgc gactttccgt
     4021 acaggtacag ttttttaaca gtgcgctttt ttgcgtgaag gcatgccaga gccggcagtc
     4081 tgccgggaac tatttcaaga ggattcaggg gcatcagagg ccgtgtactt ctttcaattt
     4141 agaagcacgc agccgtaaat tgtcattgtc ggcgacacgg ttgtcggcat catgcgcatt
     4201 ggcactgatc agatccatga ggatgttata actttcccgg taccgttcga ggcggtccag
     4261 acagcgtgcc atgcggtaca gattgtaata atagtgcctg ccttccggac catagcgcgc
     4321 cacagccagg ttcatggtgt cgtacgcctt ctgatactgc caggtgaaaa gcagataccc
     4381 gccgatacgg gtaagcccgc tttcatcgct cagatctttg gcgaagtccg cacccactgt
     4441 cgctttggta tagtggttca ccaggaaatt aacacccggg ctggtgaaga gaaatgcaat
     4501 acctgcgatc accagaataa taatcggcca tttcaaaaac gtcaaatcca ttgcctgttc
     4561 ctcctttaca taagacccag aaaagaacgc acggtaatac tgaaactgga aagatacggg
     4621 atatgaacca accatccaag caggactgcc ccgaaaaaca ggacgaccag cacgacaata
     4681 agcgaaaaca cactgccaag acgggcgcga aggcttcttt tctggcgctt cgacaaatcc
     4741 acgtcagtgg cgcgtttcat gaaatcttcg tgcttgtcgc gacactcatt ggagcagtac
     4801 atacccgccg gtgtcacaaa tccgcattct ttgcaaatcg gcttgctgca ttgacggcat
     4861 cgtcccatgg cctctttacc gggatgattg aagcaaccga caatttttgg tgctgccata
     4921 acagatactc ccttccatct gaattaccat aacggacata catctgccct gtacagtaag
     4981 tttaccatat tttactgcgt aaacgtgcag tctgcacctc cacaccatcg ctcgaactgc
     5041 tgtatctttg ccgaagccag gatatgcccg ctcccgtcat aaaacaccgc ccactgtccc
     5101 ggcgtcacag cccgctgtgc ttcagaaaga acgacccggg ccccgtgatc tctcggctca
     5161 accactcctg ccaccggttg atgccgatag cgaagctgaa cccatgcctc aaatttttct
     5221 ttttgcggtg ccattcctcc aaaaaagaga ggccccgttt caaaacaatc acatagagac
     5281 tgcgcgtctc tgccgaccca gaccgtgttg tgcgcggcat caagccagac cacgtacagc
     5341 ggcttttccg caccgattcc cagtccccga cgctgaccga tggtgtagcg gtgaatgcct
     5401 tcatgctgcc cgagtacgcg cccttccaga tcgcgaatgg gaccgggcgt gcccttcccg
     5461 gcatactttt ccactacact ggcataatta cggagcggca caaagcagat ttcctgactt
     5521 tccgctttat ttccggacac gacatctatg cgccgggcgg cagccctggc atcttccttt
     5581 gtcatcgccg caaggggaaa gcaggaacgg cgcaactgag attgggtaag gggtgccagt
     5641 acataactct ggtcttttgg caggtaagcc gcccttgcca gagtcatcct tccgtttctt
     5701 tccataagac gggcataatg cccgaaagcg atgtagtcat agccgagtcg cgttgcttct
     5761 ttaaagagca tgccgaattt gatggcacgg ttgcatcgga tgcagggatt gggcgtgcgt
     5821 ccttccgcat actgtcctgc aaaatagcgg ataatcgtct tttcaaacac ttccacgacc
     5881 cgcactgttc tgtgcggaat tcccagcgta gtgcagatac ggcgggcatc ttcagccgcc
     5941 tgcagaccgc agcagggctc aaagaccgac acttcctctt tatcacccgt cagccggagg
     6001 gttgcgccca ggcagtcata gcctgcctca gccagaagca cagccacagc ggcactgtcc
     6061 acgccaccgc tcatggcgat cataacccgg gcaccccagg gaggcatacg attttcttct
     6121 gtcgatatca ttaaaaaact ttcacggctt cctgcgccct gtatcggatg cggattgtaa
     6181 ttctcaatgc cgaaagtgta gcatgttcct aaacaacacc gcatccggat ataaacttcc
     6241 ggcatgaata ccggatcctg aaaaaccact gaaccgagac gaaggaactt acgcaatgct
     6301 gcctcttttt cttctcatga caaccgcttt tcttgctgca ggtcaaagtt ttgattcggg
     6361 agcgcccgcc atcgcccttt atgaagaggc aatggctcct gcgcacatca gcggtgccat
     6421 gaatgctccg gcgctgcatc ttttccccct ccatgaatca aaaaagcccg tgccggcagt
     6481 cgtggtatgt ccgggaggcg ggtatggcgg ccttgctacc ggccatgaag gaatggaagt
     6541 ggctcgatgg ctcaacggtc agggcatcgc cgcatttatc tgtcagtacc gcgtcgaacc
     6601 caaccgccat cctgttccgt tgcaggatgc gcagcaggca cttcgcgtgg ttcgtgcccg
     6661 tgcggaagaa tggaatgtgg atccggtccg ggtcgggatc ctcggttttt ctgcgggcgg
     6721 ccatctcgtc tccaccgtgg cgactcattt cgacggcggc gatcccgatt cggcagatcc
     6781 ggtaaaacgc cagtcaagcc gcccggattt caccgtgctt atttacccgg ttatttcgct
     6841 gctgccacct ttcggccacg taggcagcgg aaagaatctg ctcggcgata atccggaacc
     6901 cggtctggcg gaaagtctcc agaatgatca gcatgtaacc aaggacacac cgcccgcttt
     6961 tctcgtggca agcacggcgg acacaggtgt ttcggcggaa aacagcatca cctattatct
     7021 ggcactgcac cgcgccggag tgcctgcaga aatgcatgtg tatgagccgg gaccccatgg
     7081 cttcggcctg ggtaaaggcg atcccgtact gtccacctgg ccggatcttt tcatcaaatg
     7141 gcttcacacc cgggctgtac ttccctgaaa ctgaaagtgc tgaccttcaa tatttcgcca
     7201 gcgccgcacc gatgacaaac tcaggcgtga cagaccacat ctgatgcgcc ttgaatgccg
     7261 tattcatgtt ccgctcggct gcagcacaga caggctgcgc attcaatgcg gaggactggc
     7321 ggtaatagtc gagcgcctcg tcacgacgcc ccataagatc caggcagcgc ccgacccata
     7381 acagggattc cgcatgtgcg agggcatccc ggtagggata ttccgcattt ttaaggagca
     7441 gcggcagtgc ccgggcatac tttttttcct tgagaaggat cagaccggca agcctgggga
     7501 aaacaggttc cccgggaact atttccgctg cacgcagcag gtgctggacg gctcggctgt
     7561 tgtcgagatt gtccagatgg gcagaccacg cctgcagaaa ttcatagagc ccccggcgct
     7621 cctccccggt cagcggactg ccgcccggca gatcttcttt ttcatagttc ccttcatcgc
     7681 cctgcagcag tgccgacatt gaaaaaccgc agtaacggtc attgaggcac acgggaaaat
     7741 cgccgtgagc cagccagagc gtatcatcat ccgggctgaa tacaatgctc tgcacgttgt
     7801 tcgttgcggc gaccagattt ccggcaagcc ttttcctatc ctcataagga tctatgcagt
     7861 cactgagaat ggacggaccg tcttcaacac ggagtgtccc gtacttttct tcgatcagtg
     7921 ccgtgatgcg ctgaaaccgc gcttgtgtgt gacgcataaa agcgacaggt ccccgctgca
     7981 aacgctgcat gtccgccgtc acataatggt tggtgcgaac cagatactct ttttccgggc
     8041 ggatcagttc gtgatgggtg gcggaatatc cgacaatggc ggcggtacgc tctctgcttc
     8101 ctgtaatgaa aaggctcagt ccgcaaagac ggggcgctgc tgtgatacgg gcaaccgcct
     8161 cctccacggt ggtacagtaa gccagaatct catgaaccag tttgaaaatg ggagcgccct
     8221 gtgtccgcag atcggtggta aatttggagt ggagcccgac aatcaacccg ctttcattga
     8281 aaccctgccc gctgccgcag acgcccagtg cgccgatcca gcagaaagcc tgcccatcgg
     8341 agggacgcat gacaatcagc gcctgattgt cattccagac gcctctgccg aagaaatcga
     8401 aattgcgccc aacaatcagt tttccgtcct gtgttgcggc gccacaagcg aaaaagcagg
     8461 agcaggacgg aggcgaggtc agggtagtga aggtcttccc tgcaagataa tggaagatat
     8521 cgggcatgac gagaagccgt tccgcatcgg caagcggtag gttatacgct tgcgccagtc
     8581 cgcgcagatg cgccttcaga tccgccccgt agtctctgct gtttctccgc cggaacagct
     8641 cctgaatcag ccaggcagca gaacgggaaa tggacggaac cgcctgagca atcagtttgg
     8701 acaggagccc gttcatgtac agcgccacca catcgccgca ggccgctgcc gccagttccc
     8761 catgctggcg gcccatggtc tcgtagtccc cttccagctg cgccacataa atgtctttgc
     8821 gccgaagcag gacgccaccc gaagattcta tgcaaaactc cgacgactga gcactcatat
     8881 cccgcttcct tgttcagatt ccggtaaaaa acgctgatga ttcgggcagt gctatcagac
     8941 tgtgaaggca agaaattttt cgggtcgatg cctgatgaat aaaaacgctt ttt
//
LOCUS       DAOPWB010000125         8915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7848_length_8915_cov_5.367833, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000125 DAOPWB010000000
VERSION     DAOPWB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7848_length_8915_cov_5.367833"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /gene="dapB"
                     /locus_tag="PKN92_10510"
     CDS             complement(<1..714)
                     /gene="dapB"
                     /locus_tag="PKN92_10510"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNZ49268.1"
                     /translation="MITICMAGACGRMGRRILELTSGIENMAAGSALDLPALAGTEIA
                     VTLSNGKEERLVVGADSRAEIGKAQVLIDFTAPAACLEHVAVAEAMKKPAVVGTTGLS
                     AEQVEALKRFAQNIPVVYAPNMSVGVNLLFKLTEEVARRLGLAYNVEIVEAHHNLKKD
                     SPSGTAVRLAEQAARGLGLSYPEEAVFGREGIIGARPERQIGVHAIRGGDIVGEHTVS
                     FIGCGERLELTHKAHNRDNF"
     gene            complement(743..4237)
                     /locus_tag="PKN92_10515"
     CDS             complement(743..4237)
                     /locus_tag="PKN92_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNZ49269.1"
                     /translation="MAKELKQSDGYRYFPFGMLLRDQAWQDYKTEQILEKTGVDWPDD
                     PFLFAQMLAARVNVRRSAPPWVRAGELHAMILLHEIFRTLMDLYARDVKPQVFTDGLK
                     HVRQEQDAGIAESTFPAFISYFPPLQLKQSPDIPPVLFLQGSSRGKAHTVLTLQEMIL
                     VQLAHENNALQPCKSLFDYSDLCRRVPLLKVIQALDVWFSGQPALPGTGKSLLELLRE
                     PLRASPDSLEGQLDFILQHWRVFLPEQLIQRLLLVQGILREETMFRGHGPGDPAPLRF
                     SAGAYDEPEAFTADVEWMPRVVLLAKLAFVWLHQLSLQYNRPITRLSDIPDEELDRIA
                     GWGFNALWLIGVWERASASRVIKQRMGNPEAAASAYSLHDYVIAGDLGGEEAYQDLAH
                     RAGQRGIRLAGDMVPNHMGLNSKWVTDHPDRFLSLPEPPYPGYRFDGPDLSEDRRVGL
                     YIENGYWNHSDAAVVFKRVDRHSGEVRYIYHGNDGTSMPWNDTAQLNFLNAETREAVI
                     QTILEVARRFPIIRFDAAMTLAKRHYQRLWFPLPGDGGAIPSRAAHGLDKSAFDAAFP
                     VEFWREVVDRVMAEVPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLKMEDNAKY
                     RETIKNILEFSPEILQRFVNFMNNPDEDTAEAQFGKGDKYFGVATLLVTMPGLPMFGH
                     GQIEGFTEKYGMEYRRAYRDEIPEGWLIERHEREIFPLMRLRHLFSGAACFALFDLTT
                     PDGWVDENVFAYTNRLDQERALVVYNNRGTDTKGVLHTSTAINTARGDDVNLVRLSIV
                     EALALDTDSRLYTIWRDIRSGVDSLQHNASLAQKGLHLSLHGYEHRVFLFVRQICDLD
                     GSWGRLHAQLDGRCVPNMDEAYRELQLAPVLTPFRQFFTADLLRHILHEKDSTPLPLS
                     SREALHAFLEAACSLTGAAVSSDEPFDQINEMLETLRDFMSEPPLKLLPSAIQKFCMN
                     LLDKEGEKEHIPARFSPIAAAWALLRALGRSITPVEDSTALQNEVQQGEHTAAAWMHD
                     WLLIKVAAQVFAEMGGNRWQAWQDAHTLALCLRNRKTVEELEHEVWAPVLHRLFDNED
                     MHGALALHWHNMRQWINREQLEFVMESLFLINLLDQDLKEKEVVRTLVGCYENMQEIL
                     HAAADTGYDYRWMLETLK"
     gene            4443..5321
                     /locus_tag="PKN92_10520"
     CDS             4443..5321
                     /locus_tag="PKN92_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNZ49270.1"
                     /translation="MPCSMTGFARTEVMVNGGQTTIEISSVNHRFLEISIRLPGQWSA
                     LESSLRELMRGRIVRGKINVSIRLGKTLSSSAPIYVDTDRARQYIDASRTLMHLMSST
                     DSLTLDKLVTLDGVVVSDEESSDLETVLEQLSVGLEEALNQLSAMRSREGALLVEALH
                     GHLNDLEKNLARIEERAPRLVLAYEEKLRQRIAEINLEVGVKEERLAMEVALMADRMD
                     VTEELVRFRAHVLHCRELLDAEGSVGRDLNFLVQELLREANTLGSKLRDVEVSRDVIE
                     IKTEIEKLREQLQNLE"
     gene            5326..5895
                     /gene="gmk"
                     /locus_tag="PKN92_10525"
     CDS             5326..5895
                     /gene="gmk"
                     /locus_tag="PKN92_10525"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNZ49271.1"
                     /translation="MSESGIIVISAPSGAGKGTLVARILPFYPELALTVSATTRPPRT
                     GEENGREYYFLSRQDFEAGIEKGAFAEWAEVHGNLYGTFKTEIDRLLLRGRHVLLELD
                     VQGMLSMKAQYPALTSIFIVPPDMEVLKERLTRRGTEDAAVVAVRLQTAAREMERQQE
                     FDYVLVNDDLDVATEALKNLVGSILNSHR"
     gene            6033..6110
                     /locus_tag="PKN92_10530"
     tRNA            6033..6110
                     /locus_tag="PKN92_10530"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6069..6071,aa:Glu,seq:ttc)
     gene            6336..7568
                     /locus_tag="PKN92_10535"
     CDS             6336..7568
                     /locus_tag="PKN92_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNZ49272.1"
                     /translation="MRKTSALTVLLVAALCFAALPALAEQHPPVPGPDFAGCPEDAQK
                     FKGFPRFDITKDQDPVKFLDPGQYDFCKMMDNIYCSLALVVSLSADIANFANLVQCIS
                     MDINGPLNLDLKIPVQPNGVLDRYELAVLAYVLNTPSLPYYEEVKTAWEENFWELKEL
                     IVAALKAANLKSDGVQDKDITAIVNMLAPALTPSLTGVIAGFATLGDEMTNIALGELL
                     GLLEDIGLEPPEGGIAAITTGIPALGPHGDVDGDGYTNMEEYLYFVGQEGVSLDSYLA
                     AVFDPNQTPPSYDPVVTLSRKTGFIQIGDNITLDAKLKFYYDLPNFVRWYKDGVLIDG
                     ATSLQFELLNAQASDSGVYQVVVGITVEDGGKAETEISATTVLNVSDSPLPVAGIAGI
                     GALAGALTLVAARRFRRK"
     gene            7834..8091
                     /locus_tag="PKN92_10540"
     CDS             7834..8091
                     /locus_tag="PKN92_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNZ49273.1"
                     /translation="MKKGIHPENYREVVFMDVGADIKWITRSTVHTNRTIEMDGKEYP
                     LFTLDLSSHSHPFFTGKQKFVDSEGRVERFQKKFGAAKSGQ"
     gene            8098..>8915
                     /locus_tag="PKN92_10545"
     CDS             8098..>8915
                     /locus_tag="PKN92_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNZ49274.1"
                     /translation="MDGQLREKLEILVQEHGQLAEAMAKPEVATDPQRYQQHARKYAE
                     LSDLVQVFTRYKEDEKGLYGAQQLLAAEADAEMLAMAEEEAAQLQASLTALEEQLRLL
                     LVPKDPLDEKNTIVEIRAGTGGEEAALFAADLFRMYTRLAETKRWKVEVMNSNPTALG
                     GFKEVSFQLSGDNVYSCMKWEGGVHRVQRVPDTETQGRIHTSAVTVAVLPEAEEVDIR
                     IDPNDLIIDVYRSSGPGGQSVNTTDSAVRITYKPTGMVVTCQDEKSQHKNKAQA"
BASE COUNT         2015 a         2357 c         2390 g         2153 t
ORIGIN      
        1 gaagttgtcc cggttatgcg ccttatgggt cagttcgagc ctttcgccac agccgatgaa
       61 agatacggtg tgctcaccga cgatatcgcc gccgcggatg gcatgcacgc cgatctgacg
      121 ttccggtcgt gccccgatga tgccttcccg cccgaagaca gcctcttcag ggtaggacaa
      181 ccctaggccc cgcgccgcct gttccgcaag ccgtaccgct gtgccgctcg gactgtcttt
      241 tttcagattg tgatgggctt ccacgatttc cacattgtag gcaagtccca gtctgcgcgc
      301 cacttcttcc gtaagtttaa agagaagatt cacaccgaca ctcatgttgg gagcatagac
      361 caccgggatg ttctgcgcaa aacgcttcaa tgcttccacc tgttccgcag acagacctgt
      421 cgttcccacg acagccggtt tcttcattgc ttccgccacc gccacgtgtt ccagacaggc
      481 ggcaggtgct gtaaaatcga taagcacctg tgctttgccg atttccgccc tgctgtctgc
      541 accgacgaca aggcgctctt cttttccgtt gcttagcgtc acggcaattt ccgtacctgc
      601 cagcgcggga aggtcgagcg cactccctgc cgccatgttt tcaatgcccg atgtcagttc
      661 cagaatccgc ctgcccattc ttccgcaggc ccctgccata caaatggtaa tcatgaatac
      721 tgctccgtga gtcttcagtt ttttatttca gtgtttccag catccagcga tagtcgtacc
      781 cggtgtcggc ggcggcatgc aggatttcct gcatgttttc atagcagccg accagcgtcc
      841 ggaccacttc tttttctttc aaatcctgat ccagcagatt gatcaggaaa agactttcca
      901 ttacaaattc cagctgctcc ctattaatcc actggcgcat gttgtgccag tgcagtgcca
      961 gagcgccgtg catatcctca ttgtcgaaaa ggcggtgcag cacaggtgcc catacctcgt
     1021 gctcaagttc ttccactgtt ttacggttcc tcagacagag agcgagggtg tgcgcatcct
     1081 gccaggcctg ccagcggtta ccgcccattt cagcaaaaac ctgtgcagca actttgatca
     1141 gcagccagtc atgcatccag gcagcggcag tatgctcacc ctgctgcacc tcattctgaa
     1201 gggcagtgct gtcttccacc ggtgtaatcg aacggccgag tgcccggagc aatgcccagg
     1261 cggcggcaat gggggagaag cgggcgggga tgtgctcctt ttcgccctct ttgtccagca
     1321 ggttcatgca gaatttctgt atcgctgaag gcagcagctt cagcggcggc tcgctcataa
     1381 aatctcgcag cgtttccagc atttcgttga tctggtcgaa tggttcatca gacgatacgg
     1441 cagcgcctgt caggctgcac gccgcctcca gaaatgcatg cagggcttcc cggcttgata
     1501 acggcagggg cgtactgtcc ttttcatgga gaatgtgccg gagcagatca gcggtgaaaa
     1561 actgcctgaa aggtgtcaat acaggggcga gctgcaactc tctataggcc tcgtccatat
     1621 tcggcacgca ccgcccgtcc aactgtgcgt gaagtctgcc ccaggagccg tccagatcac
     1681 agatctgcct cacgaaaagg aagacgcggt gctcgtaacc gtgcagggac aggtgaagtc
     1741 ctttctgcgc cagcgatgca ttgtgctgca gtgaatccac accgctgcgt atatcccgcc
     1801 agatcgtata taaccgggag tccgtatcca gtgccagcgc ttccacgatg gaaagacgca
     1861 ccagattcac gtcatcgccg cgggctgtat ttatggcggt ggaggtgtgc agcacgcctt
     1921 ttgtatcggt gcctctgttg ttatacacaa ccagcgcccg ctcctgatcg aggcgattcg
     1981 tataggcaaa cacattttcg tccacccatc cgtcgggcgt tgtgagatca aaaagtgcga
     2041 aacaggcggc accgctgaag agatgacgca ggcgcatgag cggaaagatt tcccgttcat
     2101 gccgttcgat cagccagcct tccggtattt catcccgata ggcacgccgg tattccatgc
     2161 cgtatttttc cgtaaaacct tcgatctgcc cgtgaccgaa catgggcagc cccggcatgg
     2221 tgacgagcag tgtggcaaca ccaaaatatt tgtcgccttt gccgaactgt gcttctgccg
     2281 tatcttcatc ggggttgttc atgaaattga cgaaacgctg cagaatctcc gggctgaact
     2341 ccagaatgtt tttgatcgtt tcccggtact tggcgttgtc ttccatcttg agcatgttca
     2401 tgaatgcact gttatacacc cggtgcatcc ccagcgtccg gacaaaatag ccctccatga
     2461 gccagaaggc ttccgccaga agcagcgtgt ccggcacttc tgccataacc ctgtccacca
     2521 cttcccgcca gaattccacg ggaaaggctg catcgaaggc ggacttgtcc agaccgtggg
     2581 cagcccggga aggaatggcg ccgccgtcac cgggcagcgg aaaccacaga cgctggtagt
     2641 gccgtttggc aagggtcatg gcggcatcga agcggataat agggaagcga cgtgcaactt
     2701 cgagaatggt ctgaataaca gcctccctcg tttccgcgtt aagaaaattc agttgtgccg
     2761 tatcgttcca gggcatgctg gtgccgtcgt tgccgtgata gatgtagcgc acctcaccgg
     2821 aatgtctgtc cacccgtttg aaaactacgg cggcgtcgct gtgattccag tagccgtttt
     2881 ctatgtaaag accgacacgt ctgtcttcgg aaagatcggg accgtcaaaa cggtatcccg
     2941 ggtagggtgg ctcgggaagg gaaagaaagc ggtccggatg atctgttacc catttggaat
     3001 tcagccccat gtgattgggc accatatcgc ccgccaggcg gataccgcgc tgccctgccc
     3061 ggtgcgcaag atcctgatac gcctcttcgc cacccaaatc tcctgcaatt acatagtcat
     3121 gaaggctgta tgccgatgcc gccgcttcgg ggttgcccat gcgctgcttg ataacccggg
     3181 atgccgatgc ccgctcccat acaccgatga gccataaggc attgaagccc cagcctgcga
     3241 tgcgatcaag ttcttcgtcg gggatatcgc tcagccgggt gatagggcgg ttgtactgca
     3301 gtgagagctg gtgcagccag acaaaggcaa gcttggcaag aagtacgacc cggggcatcc
     3361 attccacatc ggcggtgaag gcttctggct catcataggc gccggctgaa aaacggagcg
     3421 gggccgggtc accgggacca tgcccccgga acatcgtttc ctctctcagg atgccctgca
     3481 caagcagcag acgctgaatc agctgttccg gcaggaaaac acgccagtgc tgcagaataa
     3541 aatccagctg tccttccagc gaatcagggc tggcgcgaag gggttcacgc agcagctcca
     3601 gcagactctt accggtacct ggcagagcgg gctgcccgga aaaccagaca tccagcgctt
     3661 gaatgacttt aagcagcggc acccggcggc acagatcact gtagtcgaaa agcgatttgc
     3721 agggttgcag tgcattattt tcgtgcgcga gctgtaccag tatcatttcc tgcagtgtca
     3781 gtacagtatg ggctttgccg cgactgctcc cttgaagaaa aaggacaggc ggtatatccg
     3841 gtgactgctt cagctggagc ggcggaaaat aggagatgaa agcgggaaaa gtgctttctg
     3901 caatgcccgc atcctgttcc tggcggacat gcttcaatcc gtcagtaaag acctgaggct
     3961 ttacgtcccg tgcataaaga tccatgagcg ttctaaagat ttcatggagc agaatcatgg
     4021 cgtgaagttc gccggcacgg acccagggcg gagcgctgcg ccgcacattg acacgcgctg
     4081 caagcatttg cgcaaaaagg aaggggtcgt cgggccagtc tacgccggtc ttttccagga
     4141 tctgttccgt tttgtaatcc tgccacgcct gatcacgcag cagcatacca aaggggaagt
     4201 acctgtaccc gtcgctttgc ttcagttctt ttgccatgga attccttcaa tgtcgccggc
     4261 tttcccgctg cagtgcatac gggatctcga aaaaacgcct gaaatatagc ataccgcatt
     4321 aatccggaag attaccattt caccggctca gaagaagatg aagtgttttc catgtgcaat
     4381 ttttcgcttt caggatggta tacttgagct ttgaactttt tatattttat aggagcagac
     4441 cgatgccatg cagcatgacg ggctttgccc gcaccgaagt aatggtcaat ggcggacaga
     4501 ccaccattga aataagcagt gtcaatcatc gttttcttga aatctctatt cgcctgcccg
     4561 ggcaatggtc agctttagag tcttcattgc gggagctgat gcgaggtcgt attgtccggg
     4621 gcaaaatcaa cgtctccatt cgtttaggaa aaacactttc ctcctctgca ccgatctatg
     4681 ttgatacgga ccgcgcccgg cagtacatag acgcctcccg tacactgatg catcttatgt
     4741 cttccaccga ttcccttacg ctggataagt tggttacatt ggatggagtg gtagtctccg
     4801 atgaagaaag ttcggatctg gagacggtgc tggaacagct ttctgttggg cttgaggagg
     4861 cattgaatca gttgagcgcc atgcgcagca gggaaggggc tcttctcgtg gaggcgttgc
     4921 acggtcactt gaatgatctt gagaaaaacc tcgcccgcat cgaagagcgt gcgccccgtc
     4981 tggttcttgc ctatgaggaa aaactccgcc agcgtattgc cgagattaac ttggaagtag
     5041 gcgtaaaaga ggaacggctt gccatggaag tggcacttat ggccgatcgc atggatgtaa
     5101 ccgaagagct ggtacggttc cgggcccatg tgctgcattg ccgtgagctg ctcgatgctg
     5161 agggttcagt cggaagggat ctgaattttc tggtgcagga actgctgcgg gaggcgaaca
     5221 cgcttggcag caaattgcgt gacgtggaag tcagccgcga tgttatagaa atcaaaacag
     5281 agatagaaaa actgcgtgag cagttgcaga atctggagta gtttcatgtc tgagtccgga
     5341 attattgtca tttctgcacc gtccggggct ggcaagggca cgttggtagc ccggatactg
     5401 cccttttacc cggagctggc tctgacggtc tccgccacca ctagaccccc ccggacgggc
     5461 gaagagaacg gcagagaata ctattttcta tcacgacagg atttcgaggc gggcatcgaa
     5521 aagggcgcct ttgccgagtg ggcggaggtg cacggtaatt tatacggaac ttttaagaca
     5581 gaaattgacc ggcttcttct gagaggcaga catgtgctgc tggaactgga tgtgcagggc
     5641 atgctcagca tgaaagcgca atatccggca ctgaccagta tttttattgt tcctcccgac
     5701 atggaagtgc tcaaggagcg cctgacccgg cggggaacag aagacgctgc tgttgttgcc
     5761 gtgcgccttc agacggctgc ccgggaaatg gagcgacagc aggagtttga ttatgtgctg
     5821 gtgaatgatg acctggacgt ggcaacggag gcgttgaaaa acctcgtagg cagcattttg
     5881 aattcgcaca ggtaaacaaa agtacctccg tgcagccttt gtaccggtat atagcgcttc
     5941 ttttctgccc gctgaggtaa aaatttgatt tcctcttttc gcttctgcta cactacgttc
     6001 catcatgaca cggttcctgt gtcatttcgt gcgtccccat cgtctagtct ggtctaggac
     6061 accgcccttt cacggcggcg acaggggttc aaatcccctt ggggacgcca tttttttctc
     6121 ccaccccctc accttttttc tctcaatttc tttcagtagt cctgtgccct tttttttatc
     6181 acggtctatt cttcttgctt tttcaaatca tctaaggtat actttatgtg gcatgtggtt
     6241 gtatcactcg atgcaattat atgttaagcg cctgtggtat gcgttgttgc tttattatgt
     6301 cttactaaat aaccaaacaa ggagtgaaag aaactatgag aaagacaagt gctttgacag
     6361 tgctgcttgt agcagcgctc tgttttgccg cactgcctgc attggcagag cagcacccgc
     6421 ctgtgcccgg gcccgacttt gccgggtgcc cggaagatgc gcagaaattt aagggtttcc
     6481 cccgattcga tattacgaaa gatcaggatc ctgttaaatt tcttgatccc ggtcagtatg
     6541 acttctgcaa aatgatggat aacatctatt gctctcttgc ccttgtggtc agcctcagtg
     6601 ccgacattgc gaactttgcc aatctggtgc agtgtatatc gatggacatc aacggccccc
     6661 tgaatctgga tcttaaaatt cctgttcagc ccaacggtgt tctggaccgt tatgagctgg
     6721 ctgtgcttgc ttatgttctc aatacacctt ccctccctta ctatgaagaa gtcaaaacag
     6781 catgggaaga aaacttctgg gaactgaaag aactgattgt tgcggcactg aaagctgcca
     6841 atctgaaatc ggatggcgta caggataagg atattactgc aatcgtcaat atgcttgctc
     6901 ctgcattgac tcctagtctg acgggtgtga ttgcgggttt tgcaacgctg ggtgacgaaa
     6961 tgaccaacat tgctctcggt gagctgctcg gactgcttga agacatcggt cttgagcctc
     7021 ctgaaggcgg tattgctgcc attacgacag gaattcctgc gctcggtcct catggcgatg
     7081 tagatggcga cggctatacc aacatggaag agtacctcta ttttgtcggt caggaaggcg
     7141 tgagcctcga cagctacctg gctgctgtct ttgatcctaa tcagacgccg cccagctatg
     7201 accctgtagt gactctctcg cgcaagacgg gatttattca aatcggggat aacatcactc
     7261 tggatgctaa gctgaaattt tactacgatc tgcccaattt tgtgcggtgg tacaaagacg
     7321 gtgtgctgat tgacggtgcg acaagcctcc agtttgaact gctcaatgct caggcaagcg
     7381 acagcggtgt ctatcaggtt gttgtcggca ttacagttga agacggcgga aaagccgaaa
     7441 cagaaatttc cgccactact gttctcaatg tgagcgacag tcctcttcct gtagccggaa
     7501 ttgccggaat cggtgctctt gccggtgcac tgactctggt tgccgcccgt cgtttccgtc
     7561 gtaaataaac gttcatcttt tgtctgaagc gtttgtattt tgtggttgtg ttttgccgcg
     7621 cgtgaagtga tctgcttcgc gcgcggcttc acagtccggc agaagagttt ggaatcaggg
     7681 cgcacagggc tgtgtaataa tttgctctat tccgtctctt tttgctatac ttcaatccta
     7741 ccctttgaga gagcacactt aatttatcta tgatcattca cggatagaag tgtgcttttt
     7801 taaaattaac ccgatgatca ggagaatagt acgttgaaaa agggcattca tcccgaaaat
     7861 tatcgtgaag tcgtcttcat ggacgtaggt gctgatatta aatggattac ccgttccacc
     7921 gtgcacacca accgtacgat tgagatggac ggcaaagaat atcctctctt tacactggat
     7981 ctttccagcc attcccatcc tttcttcacc ggcaagcaga agtttgttga cagtgaaggc
     8041 agggtggaac gtttccagaa aaaattcggc gcagcaaaaa gcggtcaata agtgcctatg
     8101 gacgggcagc tccgggaaaa gcttgagatc ctcgttcagg aacatggtca gctggcagaa
     8161 gcgatggcaa agcctgaagt agctacggat cctcagcgct accagcagca tgcccgaaaa
     8221 tatgccgaat tgtctgatct ggtgcaggtc tttacccgct ataaagagga tgaaaaaggt
     8281 ctgtacggtg cacagcagct gttggcagcg gaggcagatg cagagatgct cgccatggcc
     8341 gaagaggagg cagcgcagct ccaggcgtcc ctgacagcgc ttgaagagca gttgcgtctt
     8401 ctcttggttc ccaaggatcc gctggatgag aaaaatacca tcgtggagat tcgcgcggga
     8461 accgggggcg aggaagccgc attgtttgcc gcggatcttt ttcgcatgta tacgaggctt
     8521 gcggagacaa agcgctggaa agtcgaagtg atgaactcca atcccaccgc actgggcggt
     8581 ttcaaagagg tcagctttca attgtccggc gacaatgtct acagctgcat gaaatgggaa
     8641 ggtggggtgc atcgtgttca gcgtgttccg gatacggaaa cacagggacg cattcacacc
     8701 tcagccgtca cggtcgctgt tcttcctgaa gcggaagagg tggacatacg catcgatccc
     8761 aacgacctga tcattgatgt ctaccgatcc agcggacccg gcgggcagag cgtcaataca
     8821 accgattccg ctgtccggat cacctacaag cccacgggta tggttgtcac ctgccaggac
     8881 gaaaaatccc agcataaaaa caaagcgcag gcatt
//
LOCUS       DAOPWB010000126         8874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7898_length_8874_cov_8.985032, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000126 DAOPWB010000000
VERSION     DAOPWB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7898_length_8874_cov_8.985032"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /locus_tag="PKN92_10550"
     CDS             <1..1904
                     /locus_tag="PKN92_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49275.1"
                     /translation="NAVSVLGEDPTGTDVPGDDEVTVPADQTPGLTVTKTADAVLLPV
                     VGDSITYTITVTNTGNVTLTDVQVTDALIGLAETIATLAPGASEVFTGSYTVTQADVD
                     AGEVVNTATAMGDDPSGDPVEDEDTHVVPPVLAPELSVTKTTSAATFSAVGEVLDYTI
                     TVTNTGNVTVSNIAVNDPLTGLAEVIASLAPGESRSFATSYAVTQADLDAGAVLNAVS
                     VLGEDPTGTDVPGDDDVTVPADQDPSLSVYKEADSAYAPSVGEQIEYYITVINSGNVT
                     LTNIVVKDPLTGLHEEIPALAPGDRREFITTYTVTAADVEAGSVVNVATATGDDPNGN
                     PVEDDDSRILDVIDGFIQAVDDHYEPVNGTTGNPNAGNALDNDTLFDAPVDSSDVIIS
                     IITPASPVTPGSPVPELDTETGNVGVPAGTPEGTYTIVYKICAIYGGGSKALDGGFVD
                     GIDSLARQVTICDEATITIVVVNDAEGEEPVEGEGIAAIDLTKTAEPATYSAVGDVIT
                     YTITVTNTGDITLRNVRVTDEMVGLEEVVSEMVPGENKVFTVEYSITEADLATGEIVN
                     IARAVSETLDDEVEDEGTATVTLDDEEQGGCKGFDWGPGTLFTTILAFLAMLFSSLFG
                     GGNIRPPKV"
     gene            2074..2415
                     /locus_tag="PKN92_10555"
     CDS             2074..2415
                     /locus_tag="PKN92_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNZ49276.1"
                     /translation="MAFVVCEPCIKCKYTECVTVCPVDCFHEGENMLVIDPIECIDCG
                     VCVEECPVRAIYCDEEVPEKWQDFIVLNATYAPQWPVLRDGKGPLPSAEEWSSVTDKR
                     QEFIPRPGKGS"
     gene            2424..3734
                     /locus_tag="PKN92_10560"
     CDS             2424..3734
                     /locus_tag="PKN92_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49277.1"
                     /translation="MMKKLFLSAIFLAAAWQQFAASGMTLLEKAIIFEYDMEQRFLLE
                     GQALCKLRHPQNADDPVTYNMPDNCYMTGIYLGTMAMKYAVTQDEKDRESAVRSLNAL
                     HLLCSVSGIPGLWSRAVLPAGQPVGDDGIWRLSEDGSYQWRGDVSTDQVCGAMFGFAL
                     AHELIADDEQKKKIGEETAALIQHVLDNKMRIRDADGKPTQWGRYGPEYAARLEKLNA
                     LLWLQALKTAAHTSGLPEYADLYRSWALDQGYAQAAVRARRLLDPAIKGAVNHSDDML
                     LFLGYVPLLLLERDSEILDSVHRSVRRSWEGDEKHPGVQPEDNPFYAFIVAKYLGDTV
                     RIEEAKNTLRWFPFDMKWNTDTRQQYEKHFQVSLAFPAQSPAPEAKKPVPIDRRSRTW
                     SAWVQDPYHEIGSRDTDSLMEYNGHDYLLGYWTGRYCGFISPEE"
     gene            3776..4549
                     /locus_tag="PKN92_10565"
     CDS             3776..4549
                     /locus_tag="PKN92_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ49278.1"
                     /translation="MIRFTVLGSSSSANAVLVESADACILVDNGFTFKKLTETLTGIN
                     RSVEEIEAVLVTHEHSDHVNGVGILARKMKVPVFMTRGCALAAYPRTGEIPDLRHFES
                     GDTLSFGSLSAISFSVTHDAADPVNYVFTSGNARLGLATDFGHCSQLARARLRDVHAL
                     IVESNYCPDMLRTGKYPPQLQQRIRSRMGHLSNLDMQALLRELCGGSLKLVVLSHISR
                     DNNTPEIARSFAEEAVTGYDVAVHVAPPDRATPTFEVYP"
     gene            4546..5772
                     /locus_tag="PKN92_10570"
     CDS             4546..5772
                     /locus_tag="PKN92_10570"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ49279.1"
                     /translation="MTSLRAFFQEKQVQQMIARAAKILSIPIKLHHKSNWAIQTEGTC
                     EACRFFQEIPEGKELCAGCRMKAGRLALASNVPVTFVCHAGFTCYAAAALPGEAYFFT
                     FGPYMPEEGVHAIEAEVGRRVGELEGKRTDRTALPFDLADIRRTSDESVAAAAEWLLE
                     GLAQLYSDYVREEGDDATGFPDDMTEKNAAGLPSPEVAGEDPRLRIAAAYLLFGKTRV
                     LHAVLEDQLEETGKSPQTRQSCVIAGISKVLGYLHQCGAGIESCRDRFPEFVAAVHQE
                     DIPRGLLQLCDRFVKSVSPHKTLLKYGAELPEVLEEMERRYGEDLQLQRLAEPMQIHP
                     TTLARRLECVTGMKFGELLKRIRLMQAQRLLRRTSLSATAITRRVGIQDQSNFTKIFK
                     RYTGMTPKEYQMRYKQ"
     gene            5854..7032
                     /locus_tag="PKN92_10575"
     CDS             5854..7032
                     /locus_tag="PKN92_10575"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNZ49280.1"
                     /translation="MRLRIKVIQIGLSVLFAAAFYPAGLSAEVSGVPVFAREEGGYHT
                     FRIPALIVSHDGTLLAFCEGRKHSASDTGDIALVMKTSTDSGASWSPVQVVWDDPGNT
                     CGNPCAVVDRDTGVLWLLMTWNLGEDREDKIIRQESRDTRRVFITSSSDNGKHWSEPA
                     EITQSVKKPNWTWYATGPGNGIQKEKAPHPGRLVIPCDHIEADTHAYYSHVIYSDDHG
                     RSWHLGGSVPEPQVNECCVAELSDGSLLLNMRNYYAENKVRQKAYSTDGGVQWEGQSF
                     DFGLIEPVCQASLVRYPRQSREGKDILFFSNPASAEHRKAMTLRASTDGGQNWEYSLL
                     FHSGPGAYSSLAVLPDGSVACLYECGETHPYEQLRFVRVFPAALGLPGVPMEAGNGAR
                     "
     gene            7327..8073
                     /gene="gpmA"
                     /locus_tag="PKN92_10580"
     CDS             7327..8073
                     /gene="gpmA"
                     /locus_tag="PKN92_10580"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNZ49281.1"
                     /translation="MHKLVLIRHGQSTWNLENRFTGWTDVDLSEQGRSEAASAGKVLK
                     SEGFEFDLVYTSVLKRAIRTMWHVLDELDQMWLPVIRDWRLNERHYGALQGLNKAETA
                     AKHGEDQVKIWRRSYDVPPPPLEAADDRNAAHDRRYAGLSADQIPLTESLKETVARFI
                     PYWENDIAPQVKAGKRVLIVAHGNSLRALVKYLDGISEQDIISLNIPTGIPLVYDLDE
                     NLKPLGHRYLGDPEAIKKAAEAVANQGKAG"
     gene            complement(8074..8808)
                     /locus_tag="PKN92_10585"
     CDS             complement(8074..8808)
                     /locus_tag="PKN92_10585"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNZ49282.1"
                     /translation="MSSFHAAEYYELLGNRGGRLDREGPFLLEALASAPSNRVLDLAS
                     GLGLHAHYLAERGAEVSAYDLSEGMTEHARKQRPHPKIRYAAGDMRYPQGGPYGLALC
                     VGNSLCLLDGRDSLDLFFQGVAGVLHPGGILITQTLNYDKPALKKPRIRVEQEQNDRQ
                     HLVAVKSFQPADTQTRLTIDYFEAVSGSVRKTSEESLLQHWTAELLTECAAKAGMKME
                     SGRGSYDGAPLTPDSTDIILLYRKTD"
BASE COUNT         2092 a         2143 c         2539 g         2100 t
ORIGIN      
        1 tgaatgccgt gtctgtgctg ggtgaagatc ccacgggcac agatgtaccc ggcgatgatg
       61 aggtgacagt gcctgcggat cagaccccgg gtcttaccgt gacgaagaca gccgatgcgg
      121 tgcttctgcc tgtcgtgggt gattccatta cctacacgat tacggtaacg aatacgggta
      181 atgtgacgct tacggatgtt caggtgacag atgctctgat cggccttgcc gagacgattg
      241 ccacgctggc tccgggcgct tcggaagtgt tcaccggttc ttatacggtg acccaggccg
      301 acgttgatgc cggagaggta gtgaataccg caacggcaat gggtgacgat ccgagcggcg
      361 atcctgttga agacgaagat acccacgtgg ttccgcctgt gcttgcacct gagctgagtg
      421 tgacgaagac gacttccgca gcgaccttca gcgctgtcgg tgaagtactt gactacacga
      481 ttacggtaac gaacacgggc aacgtgacgg tgagcaatat cgctgtgaat gacccgctta
      541 cgggccttgc ggaagtgatt gcctctcttg cgccgggcga aagccgtagt tttgcgacaa
      601 gttatgcggt gacccaggcc gatcttgatg cgggcgccgt gctgaatgcc gtgtctgtgc
      661 tgggtgaaga tcccacgggc acagatgtac ccggcgacga cgatgtgaca gtgcctgcgg
      721 atcaggatcc ttcgctgagc gtctataaag aagctgacag cgcctatgct ccgtcagtgg
      781 gcgaacagat tgaatactac atcactgtaa tcaacagcgg taatgtgacg ctgaccaata
      841 tcgtggtaaa agatccgctg accggtctgc acgaagagat tcctgcactt gcaccgggtg
      901 acagacgcga atttatcacg acctataccg ttacagcggc cgatgttgaa gcaggcagtg
      961 ttgtgaacgt tgctacggca accggtgacg atccgaatgg taatcccgtg gaagatgacg
     1021 acagtcggat ccttgatgtt atcgacggtt tcatccaggc ggtagacgat cattatgagc
     1081 cagtgaacgg cactacgggc aatccgaatg ccggcaacgc tctggataac gacacactgt
     1141 ttgatgctcc tgttgacagt tctgatgtga tcatcagcat tatcacaccg gcttcgcctg
     1201 tgacccctgg cagccctgtg ccggagcttg atacagaaac gggtaacgtg ggtgttcctg
     1261 ctggtacgcc cgaaggtacc tataccattg tatacaagat ctgcgcaatt tatggcggcg
     1321 gttctaaagc gcttgacggc ggcttcgtgg acggcatcga ttcacttgcc cgacaggtta
     1381 ctatctgtga cgaggcgact attaccattg tagttgtgaa cgatgctgaa ggcgaagagc
     1441 ctgttgaagg tgaaggcatt gcagccattg atttgacgaa gacggctgag cctgcaacct
     1501 atagcgctgt cggcgatgtg atcacctata ccatcacggt aacgaatacg ggtgatatta
     1561 cattgcggaa cgtgcgggta actgacgaaa tggtcggcct tgaagaagtg gtttcggaga
     1621 tggttccggg cgaaaataaa gtgttcacgg ttgagtacag tattacggaa gctgaccttg
     1681 ccacaggtga gattgtgaac attgcccgtg ccgtttctga aactctggat gacgaagtgg
     1741 aagacgaagg tacggcgacc gtcacccttg atgatgaaga gcagggtggc tgcaagggct
     1801 tcgattgggg tccgggaaca ttgttcacga cgatccttgc cttcctggca atgctgttct
     1861 cctcactctt tggtggaggc aatattcgcc cgccgaaagt gtgattggta gtctctgatt
     1921 gatgtgcagg ccagctgttc cgttgagcag ctggcctttt ctcttatgga cagatctgta
     1981 gtgccggcat tgttcagagg tgctcttgct tcatatgatg aaaagggcat accatatatg
     2041 gggcattgcg ttaacgatac aggagacgtt tttatggctt ttgttgtgtg cgagccttgc
     2101 atcaaatgca aatataccga atgcgtaact gtctgccccg ttgactgttt tcacgaaggt
     2161 gaaaatatgc ttgtgattga tccgatagaa tgcattgact gcggtgtctg tgttgaggaa
     2221 tgtccggttc gggcaattta ttgtgatgaa gaagttcctg aaaaatggca ggatttcata
     2281 gtgctcaatg cgacttatgc gccgcaatgg cctgttctgc gtgatggaaa gggccccctg
     2341 cccagcgcgg aggaatggag ttctgtcacc gataaaaggc aggaatttat tcccagaccg
     2401 ggcaaaggtt cctgatgtat tttatgatga aaaaactgtt tctgtctgct atttttctgg
     2461 cggctgcctg gcagcaattc gccgcttccg ggatgaccct gcttgaaaag gcgattatat
     2521 ttgagtatga catggagcag cgctttcttc ttgaaggcca ggctttatgc aaactgcggc
     2581 atccgcaaaa tgcagatgac ccggtcacgt ataatatgcc tgacaactgc tatatgaccg
     2641 gaatttatct cggaaccatg gcaatgaaat atgctgtgac ccaggatgaa aaagaccggg
     2701 agtcagcagt gcgctcctta aatgctttgc atctcctctg ttctgtcagc ggcattcccg
     2761 ggctctggtc ccgtgctgtg cttcctgcag gtcagcctgt tggagatgac ggcatctggc
     2821 gcctttctga agatgggtcg tatcagtggc gcggcgatgt cagcacggat caggtctgcg
     2881 gtgccatgtt tggtttcgct cttgcccacg aactgatcgc cgacgatgag cagaagaaaa
     2941 aaatcggcga agaaactgca gccctgattc agcatgtttt ggacaataaa atgcgtatcc
     3001 gtgatgctga cgggaaaccg acgcagtggg ggcgctacgg gcctgaatat gccgcccgcc
     3061 tggaaaaact gaatgctctt ttgtggctgc aggcactgaa gactgctgcg cataccagcg
     3121 gccttccgga atatgccgat ctttaccgca gctgggcgct ggatcagggc tatgcgcaag
     3181 cggcagtacg ggcacgccgc cttcttgatc ccgctataaa aggagcggta aaccatagtg
     3241 atgacatgct tctttttctg ggatacgttc cgctgcttct gctggaaaga gattccgaaa
     3301 ttcttgattc agtgcaccgg agcgtccgac gttcctggga gggggatgag aaacatcccg
     3361 gagtgcagcc ggaagacaac cctttttatg cttttattgt tgcaaaatat ttgggggata
     3421 ccgtcaggat tgaagaagcc aaaaataccc tccgctggtt tccctttgac atgaagtgga
     3481 acacagatac gcggcagcag tatgaaaagc attttcaagt gtccctggct ttcccggcac
     3541 aaagcccggc gcccgaagca aaaaaacctg ttcccattga cagaagatcc agaacctggt
     3601 cagcgtgggt gcaggatccg tatcatgaga tcggctcgcg ggatacggat tctctcatgg
     3661 aatacaacgg gcatgattat ctgctgggtt attggacagg ccgttattgt ggttttatca
     3721 gtccggaaga gtaactcatt cgactggcag cctatgcaaa aggaaagatg gattcgtgat
     3781 tcgttttaca gttctgggaa gcagcagcag cgccaacgct gtgcttgtcg agagtgctga
     3841 tgcctgtatt cttgtggata atggctttac atttaaaaaa cttacggaaa ccctgacagg
     3901 gattaaccgc agtgtggaag aaatagaagc agtgctggtt acgcatgagc acagtgatca
     3961 tgtcaatggg gtgggtattc tggcgcgaaa gatgaaagtg cctgtcttta tgacccgtgg
     4021 ctgtgcattg gcagcctacc cccgaacagg agaaatcccg gatcttcgcc actttgaatc
     4081 gggagacacc ctttctttcg gttcgctgtc ggctatcagc ttttccgtga cgcatgatgc
     4141 cgccgaccct gtcaattatg tgtttacttc cggaaatgcg cgactgggtc tcgccactga
     4201 tttcgggcat tgttcgcaac ttgcccgggc acggctgaga gatgtgcacg ctttgatcgt
     4261 ggaatccaat tactgcccgg atatgctccg gacaggaaaa tatcctcctc agctgcagca
     4321 gcgtatccgc agtcgtatgg ggcatctttc caatctggat atgcaggctt tgctcagaga
     4381 actgtgcgga ggctcgttga agctcgtcgt gctttcccac atcagccgcg acaataacac
     4441 gcccgaaata gcacgctcgt ttgccgaaga agcggtgacg ggctatgatg tggctgttca
     4501 tgtggctccc cctgaccgcg caacgccgac ttttgaggtg tacccgtgac ttctttgcgt
     4561 gccttttttc aggaaaaaca ggtgcagcag atgatcgccc gtgcggcaaa aatcctgtcc
     4621 atcccgatta aactgcatca taaaagcaac tgggctatcc aaacagaagg aacgtgtgaa
     4681 gcctgccgtt tttttcagga aattcccgag gggaaagaat tatgtgccgg ctgtcgtatg
     4741 aaagcgggca gactggcact ggcgagcaat gttccggtta cctttgtatg ccatgcaggc
     4801 tttacctgtt atgcggcagc agcgcttcca ggcgaagcct attttttcac ctttggccct
     4861 tacatgcccg aagagggggt tcacgccata gaggcggagg tgggcaggcg agtcggcgag
     4921 ctggagggca aacgcaccga cagaacagcc cttcctttcg atcttgccga cattcggcgt
     4981 accagtgatg aaagtgttgc tgccgctgca gagtggctcc tcgaagggct ggcacagctt
     5041 tacagcgact atgtccggga agaaggggac gatgccaccg gtttcccgga tgacatgaca
     5101 gaaaaaaatg ctgccggact tccttcgccg gaggtagcag gtgaagaccc ccgtcttcgg
     5161 atagccgctg cctatctgct tttcggtaaa acccgggtgt tgcacgctgt cctggaagac
     5221 cagctggagg agacgggcaa aagtcctcag acccgccaga gctgtgttat tgcaggcatt
     5281 tcgaaagtgc tgggttacct gcatcagtgc ggggctggta ttgaatcctg ccgggatcgg
     5341 ttccccgaat ttgtggcggc ggtgcatcag gaggatatac cccgcggact gcttcagctg
     5401 tgtgatcggt tcgtaaaatc tgtttcaccg cataaaacgc tgctgaagta cggtgcggaa
     5461 ttgccggaag tgcttgagga gatggagagg cgttacgggg aagacttgca gctacagcgc
     5521 cttgcagagc cgatgcagat tcacccgacg acgctggcac gccgccttga atgtgtgacg
     5581 ggcatgaaat tcggtgaact gctgaagcgg atccggctga tgcaggcaca gagacttttg
     5641 cggcgtactt cgctctcggc tacagcgatt acgcggcgcg tgggaataca ggatcagagc
     5701 aattttacca aaatattcaa acggtataca ggcatgactc cgaaagagta tcagatgcgg
     5761 tataagcaat aatgaactat aagatctgat gcacgctgag agcgccgcta aggtgagccg
     5821 cctgatgtct gcagaaataa aagggcagtg aaaatgcgat tgagaataaa agtcatacag
     5881 atcggattgt ctgttctttt tgctgctgca ttttatccgg caggtttgtc tgcagaagtt
     5941 tcaggggttc ctgtcttcgc acgggaagag ggcgggtatc atactttcag aattcccgcc
     6001 ctgattgtca gccatgacgg tacactgctg gcgttttgtg aaggaaggaa gcactctgcg
     6061 agtgatacag gagatattgc gcttgttatg aaaacttcta cagattcagg ggcaagctgg
     6121 agcccggttc aggtggtttg ggatgacccc ggaaacacct gcggcaaccc ctgtgctgta
     6181 gtggatcggg acacaggggt tttgtggctt ttgatgacct ggaatctggg agaggacaga
     6241 gaggataaaa ttatccggca ggagagcagg gatacacgcc gtgtttttat tacatcgtct
     6301 tctgataacg gaaagcactg gagcgagccg gcggaaataa cgcagagcgt taaaaaaccg
     6361 aattggacct ggtatgccac cgggccgggg aacggaatcc aaaaagaaaa agcaccgcat
     6421 ccggggcgac ttgtcatccc ttgtgatcac attgaagcgg acacacacgc ctattattct
     6481 catgttattt attccgatga ccatggaagg agctggcatc tgggaggaag tgtgccggag
     6541 ccgcaggtga atgagtgctg tgtggcggaa ttaagcgatg gcagcctgct tctgaatatg
     6601 cgcaattatt atgcagaaaa taaagtcagg caaaaggcat acagcactga cggcggagtg
     6661 caatgggagg ggcagagctt tgacttcggg ctgatcgagc ctgtctgcca ggcatcgctg
     6721 gtgcgctatc cccggcaaag cagagagggg aaagatattc tctttttttc caaccctgcc
     6781 agtgcagagc accgtaaagc catgacctta cgcgccagta ccgacggcgg gcaaaattgg
     6841 gaatactctt tgctgtttca ttcgggtccc ggtgcctatt cttctcttgc cgttttgcct
     6901 gacggcagtg ttgcctgtct gtatgaatgc ggcgaaaccc acccctatga gcagctgcgt
     6961 tttgtccgtg tttttccggc ggcattggga ctgccgggcg taccaatgga ggccggtaac
     7021 ggtgcccggt aataggtgtt tttcctgaag aaaaacagag aggtgccctg ctatttcgtg
     7081 gctctttccc ggctgtggta tactcttaac tctgtttata agcggcatcg catatgaagg
     7141 acagaagccg ggttgcagcc ggaatgtgca ttgcgtccgg cggtgcgcaa aacaataaat
     7201 agtatgcacg gagactcgat ccgacctgtt caatcaggca tttcgggcat gcggaccggc
     7261 atcgatggat ttggcagata actgtataaa acagggcagt gcccataaaa acaagggaat
     7321 atgtttatgc acaaactcgt attaatcaga cacgggcaaa gcacatggaa tcttgagaac
     7381 cgttttacgg gatggactga tgtggatttg agcgaacagg gacgcagtga agctgcttca
     7441 gcaggaaaag tattgaagtc cgaaggattt gaatttgatc tggtctatac ttcggtgctg
     7501 aaacgcgcta tccgcaccat gtggcacgtg ctggatgagc tggatcagat gtggcttcct
     7561 gttatccggg actggcggct gaatgagcgt cactacggtg cgcttcaagg gttgaataaa
     7621 gcggaaacgg ctgcaaaaca cggggaggat caggtcaaaa tctggcggcg ttcctacgac
     7681 gttccccctc cgccgctgga agcggctgac gacagaaacg cagcccacga ccggcggtat
     7741 gccggactca gtgccgacca gattccgctg actgaaagcc tgaaggaaac agtggcgcgg
     7801 ttcattcctt actgggaaaa tgatattgcg ccgcaggtaa aagcagggaa gcgagtcctg
     7861 attgttgctc atggcaacag cctgcgtgca ctggtcaaat atctggatgg aatatccgaa
     7921 caggacatta tcagcctgaa catccccacc ggtattccgc ttgtgtatga tctggacgag
     7981 aatctgaagc ctctgggaca tcgctatctg ggcgatcccg aagccatcaa aaaagcagcg
     8041 gaagcagtgg cgaatcaggg caaggcaggc tgatcagtcc gttttccggt aaagaagaat
     8101 gatatcagtg gaatccggtg ttaaaggcgc accgtcgtag gagcctctgc cggattccat
     8161 tttcattccc gcttttgccg cacactccgt aaggagctca gcagtccagt gctgcagaag
     8221 agactcctcg ctcgttttgc gtacagagcc cgaaactgcc tcaaaataat caatggtcag
     8281 tctggtttgt gtatcggcgg gctgaaagga cttgacagct accagatgct gcctgtcatt
     8341 ttgctcctgt tccacccgga tacgcggctt cttcagggcg ggcttgtcat aattcagcgt
     8401 ctgggtaatc agaataccgc ctgggtgcaa taccccggca actccctgaa agaaaagatc
     8461 cagactgtcc cgtccgtcca gcaggcacag ggagtttccc acacacagcg caagtccata
     8521 aggaccgccc tggggatatc tcatgtctcc tgccgcataa cggattttcg gatgcgggcg
     8581 ctgctttcgt gcatgttccg tcatgccctc actcagatca taagcggaaa cttctgcgcc
     8641 tctttctgcg agatagtgtg catgcagtcc aagaccgctt gccagatcca gcacccggtt
     8701 tgaaggagcg ctggcgagcg cctcgagcag gaaaggacct tcccgatcaa gacgcccgcc
     8761 tcgatttcct aacagttcat aatattcagc tgcatgaaaa gaactcatga actcatttta
     8821 tccttgggat catgtactga ttacccgagc atggcagcca gatcggcctc agga
//
LOCUS       DAOPWB010000127         8816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_7965_length_8816_cov_5.135829, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000127 DAOPWB010000000
VERSION     DAOPWB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7965_length_8816_cov_5.135829"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1981
                     /locus_tag="PKN92_10590"
     CDS             <1..1981
                     /locus_tag="PKN92_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNZ49283.1"
                     /translation="LIEVPLGMTLKDIIYDVGGGAPMGRAIKAVQTGGPSGGCLPAEK
                     FDLPVEYEALAEAGSIMGSGGMIVLDEQTCIVDLVRYFLTFTQNESCGKCPPCRVGTR
                     AMLAIVTSIAEGRATSQDLATLESIARTVKQGSLCGLGQTAANPVLCTLQYFREEYEA
                     HILEKRCPAGVCAALTRTNCMSACPAGVYIPGMLALASQQRYDEALQLHRERNPFASV
                     CASICTHPCENRCERRLTDASVSIQGISRTALAHESAPMAPSVQVNSENAQRSVAIVG
                     GGPAGLSCAYFLARLGYKPVLYEAGEEPGGLLLSAVPDFRIPKETLRREIEMICSLGV
                     EVRTGQRLGRDFSLESLRTEGCEAVFLACGAASGAGLKIPGENFDGVVEALTYLSDSE
                     KKQGIAAGKRVLVVGGNDAAVDAARRAKQEGAAEVSLLYRRTRREMTARDEYLEIAEQ
                     EGIRIYSLVSPVEVLGKSGYVTALRCVRMELMEHDISGRRIPRAVEQSEFDLDADLVI
                     LAVGREADPSLSGEIASLERDASGFIKTDSRASVIGETWIFAGGEVVTGPGLAISAVA
                     EGERAAVAIDRFLSGGEHAFWRKRHTLDIRFDPEADLTLTKRMVVDRQMIEKDKDSFE
                     ELVLPFTEEIAAGQAARCLRCDFCGDDLNRQGAVS"
     gene            1999..4518
                     /locus_tag="PKN92_10595"
     CDS             1999..4518
                     /locus_tag="PKN92_10595"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNZ49284.1"
                     /translation="MIELTINGNSVHAEEGETLLKCALRHGIEIPHLCNHPSLPPYGA
                     CRICMVEVEGMRGFPTSCTTPAASGMVVRTDTPALQELRRNILGLMMLEHPSACLLCG
                     RRDLCDEFRPQAEKVGRTTGCHTCNNKEVCEVRSLSEELGFSELPVPPLYHHRPIERS
                     DPFIDRDLNLCILCGRCVRVCKHQHGTSIIEFVGRSSISRIGEAFGRTLLEADCRFCG
                     SCVDVCPTGSLADRYAKWFGKAESTAETTCLFCDEGCALSLGIQQGKVVHAQAVDPDK
                     PLCVLGRFAVAPFMNGFDRLSVPQLRVEDSLREVSWDEALAGAGEKLLPWKGETFALV
                     FDSALPLEDKFYLKAFTEQVMQSPHALEAVPDSKGKAKVVLPHGVKAVLSLGSFIDPA
                     EAEGLELLILQDVYPGALIEKASVVFPAAMFTEVAGTTVDASGTARPLFAATVPPGKA
                     RSDRQIVMDLAGAMHETVLSDLEEKLAASLKATGDPVLQCIRKTVPPAAADPSLRRDW
                     FRGRYLPGLIGGLRSLEDGSSFEEKPAPLPSDNRDPAAPPQLFQIIRKREVAPNNHEI
                     VFYAPSVAKKAQAGQFVIVMADEKSERVPYTLCDWDAEAGTITLVVQEKGRSSRKLAL
                     MQAGECAAHIVGPLGTPLDIQNFGTVALLGGCYGIGAHIANAKALKEAGNEVLIIMEA
                     RSHYLHYYLEELAAVADELIVTTIDGSNGIKGHAIDALLRRMQSGARIDRAITVGCPF
                     MMMVASKETKETGLPMFAALNPIMLDGTGMCGACRVTVQGETKFACVDGPFFDAHQID
                     WDELKDRRNAYTDAELNSLLTTEPVAHAHHAHSGGCGCGRS"
     gene            4611..6004
                     /gene="gltA"
                     /locus_tag="PKN92_10600"
                     /pseudo
     CDS             4611..6004
                     /gene="gltA"
                     /locus_tag="PKN92_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
     gene            6100..7281
                     /locus_tag="PKN92_10605"
     CDS             6100..7281
                     /locus_tag="PKN92_10605"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNZ49285.1"
                     /translation="MNKVICSLCFLLLAGFFAGSSDSPGLETGDPVPAENIILYHDPQ
                     FYAAFPSIVQRTDGELICAFRRAPDRRVFGEKGNSHTDPNSYLVLVRSTDNGRTWSRE
                     PELIYAHPFGGSQDPCLLQLTDGTLLCTSYGWARVDDSVKENFSASLRHGAFVFMGGY
                     IVRSEDGGHEWSNAILPPPLPGDTAVSAFSKPVPAYNRGAPIQGKDGRIYWAVAGQSG
                     TDTRRTATHLMISPDGGLNWDYACPIASDEKVAFNETSMYESPEGTLIAFMRTESMDD
                     HTVLARSVDGGKSFLPLEDTGWQGHPHFALPLPDHRVLLVYGYRHDPWGIRARVLNAE
                     CTDAATADEIVLRADGLNTDLGYPWATMTADGRILVVYYFHDQNNVSHIAGTFLDLPK
                     K"
     gene            7379..8236
                     /locus_tag="PKN92_10610"
     CDS             7379..8236
                     /locus_tag="PKN92_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ49286.1"
                     /translation="MNLCLSLYMLVAMSVSAFSPDEVFFQAHRGGLREVPENTLAAYR
                     YAWSLGAIPEVDICSTLDKIIICLHDDTLKRTTNATEKQDVPVSQLTFEEIRQWDAGS
                     WFSPEYAGEKVPTLEEVLAEMMKHDTASIYLDLKNVDLRQLAALIAKYDAAERIIFCH
                     NKEENCRTMAEAVPGLRTMLWIGGKVDQIRQKFNTVADEGFKGLNQVQLHLHRNKEVA
                     DSIEYLMPIEFLQEAMQRTAAHGIDLEVLPFEFDCASLSALLDLGIRWFATDEPKRFT
                     ECLKEKAQN"
     gene            complement(8350..8733)
                     /locus_tag="PKN92_10615"
     CDS             complement(8350..8733)
                     /locus_tag="PKN92_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49287.1"
                     /translation="METADPITPHNDPYWMLLLMDADRNGTTGWLGYDFIINLEIMDS
                     GRTTVKMRRNDEWHTIGDAHYAVQGNRMELSVPRKLVNQSESTPCFDFHWADNIQSFD
                     SVAELGLNGDHAPNRRWNYRFQVAD"
BASE COUNT         2045 a         2211 c         2477 g         2083 t
ORIGIN      
        1 tctgattgaa gttccgctgg gcatgacgct gaaagacatc atttatgatg tgggcggcgg
       61 tgcgcctatg gggcgtgcca ttaaagccgt gcagacgggc ggaccctccg gcggatgtct
      121 ccctgccgaa aaatttgatc ttcctgtcga gtatgaggcg ctggcggaag cagggagcat
      181 catgggctcc ggcggcatga ttgttctcga cgaacagact tgtatagttg atctcgtccg
      241 ctattttctt accttcactc aaaacgagtc ctgcggaaaa tgtccgccct gccgggtagg
      301 tacccgggca atgcttgcca ttgttacaag cattgccgaa gggcgcgcca ccagtcagga
      361 tctcgccaca ctggaaagta tcgcccggac ggtgaagcag ggctctctct gcgggctggg
      421 ccagacagcg gctaatcctg ttctctgcac gttgcagtat tttcgcgaag aatacgaagc
      481 acatatcctg gaaaaacgct gtcccgccgg tgtctgtgcg gcgctgaccc ggacaaattg
      541 catgagcgcc tgcccggcag gcgtgtatat tcccggcatg cttgcccttg ccagccagca
      601 acgctatgat gaagcgttgc agctgcatcg ggagcgtaat cctttcgcat ctgtttgcgc
      661 gagtatctgc acccatcctt gtgaaaaccg atgcgagcgc cgcctgactg atgcgtccgt
      721 ttccatacag gggataagcc gtactgctct tgcccatgag tctgctccga tggcaccgtc
      781 tgttcaggtg aactctgaaa atgcgcagcg cagcgtggcg atcgtaggcg gcggccctgc
      841 cggtttgtcc tgcgcctatt ttctggcgcg ccttggatac aagcccgtgc tttatgaagc
      901 aggtgaagag ccgggcggtc tgctgctcag tgctgttccc gactttcgga ttcccaaaga
      961 aacgctgcgc cgggaaatag agatgatttg cagtctcggc gttgaagtgc ggacaggaca
     1021 gcgcctgggc agagattttt cactggaatc ccttcggaca gaaggctgtg aagcggtatt
     1081 tctggcttgc ggtgctgctt ccggcgccgg gctgaaaatc cccggagaaa atttcgatgg
     1141 tgttgtggaa gcgctgacat atttgagcga cagtgaaaag aagcaaggga tagcggcagg
     1201 aaaacgggtt cttgttgtag gcggaaatga tgcggcagtt gatgctgcac ggcgggcaaa
     1261 gcaggaaggt gcggcagaag tatcgctcct ctaccggcgc acccgccgtg aaatgacagc
     1321 gcgggatgag tacctcgaga ttgccgagca ggaaggtatc agaatttatt cgctggtcag
     1381 ccctgtggaa gtacttggca agtctggata tgtcacggca ctgcgttgtg tccgcatgga
     1441 actgatggaa cacgatatca gcggacgccg gataccgcgg gctgtcgagc aatcagaatt
     1501 tgatctcgat gccgatctgg ttattcttgc tgttgggcgt gaagccgatc cttctctgtc
     1561 cggagaaatt gcatcgctgg aacgggatgc gtccggattt attaaaacgg attcccgtgc
     1621 atctgttatc ggggaaacct ggatttttgc aggtggtgaa gtggtgaccg gtccgggact
     1681 tgccatttca gccgtagcgg agggcgagcg ggctgccgta gccatcgacc gttttctgag
     1741 cggtggcgaa cacgctttct ggcggaaaag gcatacgctg gatatacgtt ttgatccgga
     1801 agcagatctg acgctgacga agaggatggt ggttgacagg cagatgattg aaaaagataa
     1861 agacagtttt gaagaactgg ttctgccttt tacagaagaa attgctgccg ggcaggcagc
     1921 ccgttgtctg cgctgtgact tttgcggcga cgatcttaat cggcagggag cggtatcatg
     1981 aatgacagga atccagcaat gattgaactt accattaacg gaaattctgt acatgccgaa
     2041 gaaggggaaa ccctcctgaa atgtgccttg aggcacggca ttgagattcc gcatttgtgc
     2101 aaccatccga gcctgcctcc ttacggtgcc tgccgtatct gcatggtcga agtggagggc
     2161 atgcgcgggt ttccgacttc ctgtaccacg cctgctgcat ccggcatggt cgttcgtacc
     2221 gatacgccgg cattgcagga gctgcgccgc aatattctcg ggctgatgat gctggaacat
     2281 ccgagcgcat gcctgttatg cggccgtcgg gatctctgtg atgaattccg gccgcaggcg
     2341 gagaaagtgg gacgcaccac aggctgccat acctgtaaca ataaggaagt ctgcgaggtc
     2401 cgcagccttt ccgaagaact gggtttttcc gaactgccgg ttcctcccct gtatcaccac
     2461 cggcccatag aacgatctga tccttttatc gaccgtgatt tgaacctgtg tattctctgt
     2521 ggcagatgcg tccgggtatg caagcatcag cacggcactt ccattataga atttgttgga
     2581 agaagcagta tttcgagaat tggggaagcc ttcggccgga cactgctcga agcggactgc
     2641 cgcttctgtg gctcctgtgt ggatgtatgc ccgacaggca gccttgccga ccgctatgca
     2701 aaatggtttg gcaaagcaga aagcaccgca gaaacgacct gtcttttttg tgatgaaggt
     2761 tgtgcgcttt ctctgggtat acagcaagga aaagtggttc atgcgcaggc tgtggacccc
     2821 gacaagcccc tctgtgtact ggggcgtttt gctgttgcgc cttttatgaa cggctttgac
     2881 aggctttctg tgcctcagct ccgtgtggaa gactcattgc gcgaagtctc ctgggatgaa
     2941 gcgcttgccg gagcagggga gaaactgctt ccctggaaag gggagacctt tgcgcttgtc
     3001 tttgacagtg ccctgcctct ggaagataag ttctatctta aagcctttac agagcaggtg
     3061 atgcagtctc cccatgcgct ggaagcagtc cccgattcaa aagggaaagc taaggttgta
     3121 ctgcctcacg gagttaaagc ggttctttct ttaggcagtt tcattgatcc cgcagaagca
     3181 gaaggtcttg agctccttat tctgcaggat gtgtatcccg gcgcactcat cgaaaaggca
     3241 tccgttgttt ttccggctgc catgtttacg gaagttgccg gaacaaccgt cgatgcttca
     3301 ggaacggcac ggcctttgtt tgctgccact gttccgccag gaaaagccag gtctgacaga
     3361 caaatagtga tggatcttgc aggagcaatg cacgaaaccg tgctttccga tcttgaagaa
     3421 aaacttgccg cttcgctgaa agcaacaggg gatcctgttc tgcaatgtat ccgcaaaact
     3481 gtgccgccag ccgctgctga tccgtctctg cgcagggact ggttcagagg acgctacctc
     3541 cccggcctta tagggggatt gcgcagtttg gaggacggat cctcctttga agaaaaaccg
     3601 gcgcctcttc catccgataa cagagatcct gctgcgcctc cgcagctttt ccagattatt
     3661 cgcaagcgcg aagtagcccc caataaccat gagattgtgt tttatgcgcc gtctgtggcg
     3721 aaaaaagccc aggcagggca gtttgttatc gtgatggcag atgaaaaatc agaacgggtt
     3781 ccttatacac tttgtgactg ggatgcagag gcgggaacca tcacgcttgt ggtacaggaa
     3841 aagggcagaa gcagccgcaa actggcgctg atgcaggcag gcgaatgtgc cgcccatata
     3901 gtcggacccc tgggcacacc tctggatatt cagaatttcg gcacggttgc tcttctgggc
     3961 ggctgctatg gaatcggtgc tcatatcgcc aacgccaagg cattgaaaga ggcgggtaac
     4021 gaagtgctga tcattatgga ggcaaggagc cattatcttc attattatct ggaagagctg
     4081 gcggcggtag cagatgaatt aattgttacg accattgacg gttccaacgg cattaaaggc
     4141 cacgccattg atgccctgct tcgccggatg cagtcaggtg cacggattga ccgtgccatt
     4201 acggtcggct gtcccttcat gatgatggtg gcatccaaag aaactaaaga aacagggctg
     4261 cccatgtttg cggcgctcaa tcccattatg ctggacggta cagggatgtg cggcgcctgc
     4321 cgtgtcacag tgcagggcga gactaaattt gcatgtgttg acggcccctt ctttgatgcg
     4381 caccagatag actgggatga actgaaagac cgccggaatg cttatacgga tgctgaactg
     4441 aactctctcc ttacaacaga acctgttgct catgcccacc atgcccacag cggcggctgc
     4501 ggctgcggca gatcctgaac ataataggaa agcaagccat gtcagaaatg gaaaaacaag
     4561 agaatgctgc tgcaccgaag cccaaaaaag tccggcctcc acggcagccc atgcccgagc
     4621 aggcgccgga agatcgtgcc cggaatttcc gtgaagtgcc ctacgggtac acaccggaaa
     4681 tggcgatggt cgaagcaagc cgctgcctcg actgcaaaaa gcccaaatgt gtggaaggct
     4741 gcccggtgca tgtggatatc cccggcttca tccgcctcat tcagcagggt aaatttgtag
     4801 agtctgcgca gcacctgaaa gaacagaatg cgctgcccgc catctgcgga cgcgtgtgtc
     4861 ctcaggaaga gcagtgtgaa agcctttgta ttatcggtat aaaaaatgag ccggtcgctg
     4921 tggggcgact cgagcgcttt gttgccgatt acgaacgcaa cgcaggcgca gtggcgatac
     4981 ctgaacttcc gcctgctaca gggaagcgca tagccgtgat tggtgccggt ccggcggggc
     5041 tgacagcagc cggagatctc gtgcgcatgg ggcatgccgt tacagtattt gaagcacttc
     5101 aggaaccggg cggggtgctt atttatggta ttcccgagtt ccggcttccc aaagaaattg
     5161 tcaaggcaga agttgattat cttcgcaaac taggcgtcga gtttgtcatg gacttcgtag
     5221 tgggcagaaa tgcttccatt caggagctgc ttacggaaga aggatatcac gctgttttca
     5281 tcggaagcgg tgctggactg cccgcatttc ttggtattcc ggagaaaatc tggtgggcgt
     5341 ttacagcgcc aatgaatatc tgacccgatc aaacctgatg aagtcctatc ttttccccaa
     5401 atatgatacg cccccgatcc ggcgacagcg tgtggcagtt gtcggcgggg gtaatgttgc
     5461 catggatgca gcccgaactg cattgcgcct gggcggggat gtgcatatcg tctatcgccg
     5521 tgcaaaagaa cagatgcccg cacgaatcga agagattcac cacgccgaag aagaaggcgt
     5581 gcatatgaaa ttactgacca atcccaaaag aatcatcgga gatgaccagc accgggtaac
     5641 cgggatagag tgcattcaga tggagctcgg cgaacccgat gcttcaggac gccgcagacc
     5701 tgtacctgtg gagggatctg agtttgttat cgaagcggat accgtggtga ttgcaatcgg
     5761 aaacaacccg aatcctctga ttccacgaag catgccggat cttgccgtct cccgctgggg
     5821 aacaatcctg gttgatgaag agacgatggc aagttctgtt cccggcgttt ttgctgccgg
     5881 agatatagta tccggtgctg caactgtaat cagtgccatg ggccaggcgc gaacggcggc
     5941 tgcgaccatc aacagatatg tgcgcggtga agatattgtc tttccgtcga cgaaaaacaa
     6001 ttgaagaaaa actcctgttg catcccggcg cgtttttgta ggtatagata cgctgttttt
     6061 gttctgttgc gcttctgcga ccaggaaagg gtctttctca tgaataaagt tatttgctct
     6121 ttatgcttcc tcttgcttgc aggctttttt gcgggaagtt cagacagccc gggtctggaa
     6181 acgggggatc cggttccggc agaaaacatt attctttatc acgacccgca gttttatgcc
     6241 gcttttcctt ctatagtgca gcgcaccgac ggtgagctga tctgtgcctt caggcgggcg
     6301 cccgaccgca gagttttcgg cgaaaagggc aatagccata ccgatcccaa cagctacctg
     6361 gttttggtgc gttccacgga taacggcaga acctggagtc gcgaaccgga actgatttat
     6421 gctcacccct tcggtggttc tcaggatccc tgcctgcttc aactgacaga tggcacgctg
     6481 ctttgcacca gttacggatg ggcaagggtg gatgattcag tcaaagagaa cttcagtgct
     6541 tcgcttcgtc atggcgcctt tgtattcatg ggtggttata ttgttcgctc tgaagacgga
     6601 gggcatgaat ggagcaatgc catcctgccg ccgcctctcc ctggagatac cgctgtttcc
     6661 gctttcagca aacccgttcc cgcatataac cgcggggcac cgattcaagg gaaggatggt
     6721 cgtatttatt gggcagtggc aggtcagtcg ggcacagata cccgccggac ggcaactcac
     6781 ctgatgatat ctccggacgg ggggctgaac tgggactacg cctgcccgat tgcttctgat
     6841 gagaaggtcg ccttcaacga aacatcaatg tatgaaagcc ccgaaggaac gctgattgcc
     6901 tttatgcgga cagaatccat ggacgatcac accgtacttg cccgttctgt tgatggcggt
     6961 aaaagttttc tccctctgga agataccggt tggcagggcc atcctcattt tgcgcttcct
     7021 ctgcccgatc accgggttct tctggtgtac ggctacaggc acgatccctg gggtattcgt
     7081 gcccgtgtcc tgaacgctga atgcacggat gcggcaacag ccgatgaaat tgttcttcgt
     7141 gcggacggat taaacacaga tctgggatat ccctgggcaa ccatgacggc tgacggaaga
     7201 attcttgtgg tgtattattt ccatgatcag aacaatgtca gccacatagc agggactttt
     7261 ctcgatcttc cgaaaaagtg acgctccttt agcagacatc tagaaataaa aagcctcttc
     7321 cctgcagtta ctctttttaa acaaactgta accgcagcag tcagtaaagg aaatttgaat
     7381 gaatttgtgc ttgtcattgt atatgctcgt ggctatgtct gtctctgcct tttcgcccga
     7441 tgaagtgttt tttcaggcgc accggggcgg tctgcgtgaa gtgccggaaa acaccctcgc
     7501 cgcctatcgt tatgcctgga gcctgggtgc cattcccgaa gtggatatct gttctaccct
     7561 tgacaaaata atcatctgcc tgcatgatga caccctgaag cgtacaacca atgccactga
     7621 aaaacaggat gtgccggtgt cacaactgac ctttgaagaa attcgccagt gggatgccgg
     7681 ttcctggttt tcacctgaat atgcggggga gaaagtgccc acactcgaag aggtgctggc
     7741 tgagatgatg aagcacgaca cggcttccat ttatctggat ctgaaaaatg tggatctccg
     7801 acagcttgcc gcccttattg caaagtatga cgctgccgaa cgcatcattt tctgccacaa
     7861 taaagaggaa aactgccgaa ccatggcgga agcggtaccg ggcttgcgta ccatgctctg
     7921 gatagggggc aaggtagacc agatccggca aaaatttaat actgtcgctg atgaaggctt
     7981 taaagggctg aatcaggtac agcttcatct gcatcgaaac aaagaggtgg cggattcaat
     8041 cgaatatctg atgccgattg aatttctgca ggaagcgatg caaagaacag cggcgcatgg
     8101 cattgatctg gaagtcctcc ccttcgagtt tgattgtgcg agcctgagtg ccttgcttga
     8161 tcttggaatc cgctggtttg caaccgatga gccgaaacgc tttacagagt gcctgaaaga
     8221 gaaagcgcaa aactgaaaaa aggagacggc ttttcaacag catctgaaag aggcttattt
     8281 aaaaatgctg agcggatgct gcctttaccg ttacagataa agcacactga aaaaccatct
     8341 gggtgaaagt cagtccgcga cctgaaatcg gtagttccaa cgcctgttgg gtgcatggtc
     8401 gccattcagc cccagttccg ccacgctgtc gaagctttga atattgtctg cccagtgaaa
     8461 atcaaagcaa ggcgtgctct ccgactgatt caccagttta cgggggacag acagctccat
     8521 tctgttgccc tgtacagcgt aatgcgcatc tcctatggta tgccattcgt catttcgccg
     8581 catttttact gtagtgcgtc cgctgtccat aatctccaga ttaataatga aatcatagcc
     8641 cagccatccg gtagtaccgt tccggtcagc atccatcagt aaaagcatcc aataaggatc
     8701 gttatgcggt gtaatgggat ccgccgtttc catgtagaaa aagagattgt ctccgtcaaa
     8761 agcgcgggtt caaattcgaa acgcaattat tgagaaacaa aaaaggagac attgcg
//
LOCUS       DAOPWB010000128         8495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_8340_length_8495_cov_5.978318, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000128 DAOPWB010000000
VERSION     DAOPWB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8340_length_8495_cov_5.978318"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PKN92_10620"
     CDS             <1..1191
                     /locus_tag="PKN92_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNZ49288.1"
                     /translation="LLCAVSAAVLLLQAGITHPERISTHRGWLAVHGGLHLLPLLFVT
                     GCLRDLILAMLAGLNRYGWRAGGITLASLTQVAAVWVFIWQGGGGLTALVIALAASYA
                     LALALLWIGLGSAAVPRFSQRHYREAVLFSAPLYLNQVMNFFHQRFDTVLVSLLAGVG
                     YGAVYEMLKRIPLIVNRTLNALLVPYLPHISRLIAREDRTQAAKVLNEAAALSAFIGY
                     GAALFFTALQTTLIPFLFNKEYLTGSTTLFLLLLAASLALQSGVMAQMLIALGRNLSV
                     PIVNMSTMALCLFLDLLLIPRWGLAGAAGAAAFSSGCSYALMVFWARYNGIRISLRSC
                     ALPLLLLSISALSLYYEMQNALFRLLPPVLFTVLCLMTGVTSPGRLHAITKALLPGSF
                     SASD"
     gene            1231..2307
                     /locus_tag="PKN92_10625"
     CDS             1231..2307
                     /locus_tag="PKN92_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNZ49289.1"
                     /translation="MTQSPCRIINATAPIRICDLGGWTDTWFSGHGSILNIGVYPYAE
                     AQLFVRPGDRPGKREVMIHAENFGERYALPTPIEYDKHPLLEACLDIMELPDNLDFEV
                     NLFSIAPPGCSTGTSAAISVALLGALDLLTPGRMTPHEIALLAQRVETEKLGLQCGIQ
                     DQLCSAYGGICYIDMYEYPNASVSQLWVPNRIWWELERRLVLVYLGRSHSSSEVHGKV
                     IAGFEAANSDKSALEPLRRAATAAKDALYQGDFLAFGKAMKDNTEAQRNLHPALISAE
                     AEAVIDLARKHGALGWKINGAGGQGGSVTILCGPENTGKRNFIKELSSINPAFEALPI
                     YLSRFGLRQWKSPMQDQSSFSGSS"
     gene            2304..2963
                     /gene="lipB"
                     /locus_tag="PKN92_10630"
     CDS             2304..2963
                     /gene="lipB"
                     /locus_tag="PKN92_10630"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNZ49290.1"
                     /translation="MIPIHFISIATPVPYDAMLQRQEQMRDSVHAGDDDASVFLLEHE
                     PVITLGRRAQAEHVRATPEQLHALGIIVHKSDRGGDVTYHGPGQLVVYPVLDLKRWHL
                     SVDWYLRSLEEVVIRFLSCYGLQGRRIDGLTGIWIGEQKVAAIGISIRHWICWHGIAL
                     NVAPKEEHWQTIIPCGIRDRGITSLRAHLAQVPPMQEVKNTMITCFKEVFPCAARISQ
                     E"
     gene            2965..4266
                     /locus_tag="PKN92_10635"
     CDS             2965..4266
                     /locus_tag="PKN92_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNZ49291.1"
                     /translation="MKIGPRLRSSIIFLAAALLLGIAFRTGSTHLAFSIYAFLLLLAT
                     SWMSTALWFPGLTSERTIDRLMVQQGENIQVTVKVLNKRGLPVPWLFVEDPCSLPFPV
                     RGESKRLCMLMPGQSVTLSYTLTCTRRGYHRIGPTVFESGDLFGLHKRFRTASKRDYV
                     TVLPTIAYIDTYAVSSRRPQGPVRITNRIYDDPSRINNLREYQPGDPLSKIHWKATAK
                     TGELIVKTFEATSVTGGTLVLDLHEDSYLEETRESRMELAITTTASIAYLLQMSGEQT
                     GLLTNGRDAAEVALYEGKTLDARSRFELEELRRLEMDSTRISPLTVQTQRSPNQAIKI
                     AENLARILPGQALDLPALLTAQCTGLPRDATLLPVTGQITDRLALTLGMLKQAGFQIA
                     VFLIDAGTAREEAEKLLAQHSIYMFHIASERDLHNISLSKI"
     gene            complement(4294..7623)
                     /locus_tag="PKN92_10640"
     CDS             complement(4294..7623)
                     /locus_tag="PKN92_10640"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49292.1"
                     /translation="MRSSAFLSLSFSLCFLLLSAGLPAQELQPLHAIHGFSTPECVVA
                     DPETGFAYVSNIASAPEGYWTDTGAGFISRISPDGKLDQLKWVESTEAFPLHQPKGMA
                     ILNGVLYAADNRRVVAYSLADGSFRKSYAPAGAGRLNDMAAGDGAVYVSDVEKGHIWR
                     VDISGAGNHALVGEIEGVNGVTVHGEKLYAVSWTLHEIYEVDPAGQNAPVPFGLAEHF
                     TNLDGIEVTPSGDFIVSDFVGNKICIITADRKSVRTLHTLQSPADIGTAWHENRIYIP
                     QFDDNVVIIAEFPGARETLPLSAVRDAEYRELRRQIAARPEWNNERLAREALRPDSLI
                     HDTDRSPTGIVWRRTRALLDYLSARYPDRDYSGMEAALSELAPLVAKIEEGQGAVGLQ
                     EERLCFHRLCALRREIAFSNPLLNFDRILFLKRHRAIYEHMCDQFYGIAARPGGGLFV
                     LENAFTDAPVTKDLLADSVVESGRLAGEKLHGGTAAPDLYYDGEGLLTEGADAGGGAF
                     LSPDLSFDGTRILFAYVECRGDKRHKYHREDQANYWKEPWDPGRAYHLFSVNADGTGL
                     TQLTDGPWNDMHPCWLPDGRIVFTSERRGGFLRCGRVCPTYTLFQMDSALKEILPFSY
                     HETHEWLPSVDNNGMVVYTRWDYVDRDSDIAHHLWLVYPDGRDPRGPHGNYPAVRESR
                     PWMEMSLRAIPGSHKYAATAATHHGQFFGSIITIDLHLEDDQSTGQIRRVTPEAKFPE
                     AESAPGVPRSPQGGGGDGQVYGTPWPLSEDFYLAVYDPDQSKHDLYLVDSFGNREILY
                     RDDSIGCSTPIPFRARPRPPIIPVQTHYETVAQAEPPTGVISVMNIYETRREWPENTR
                     IKELRLIQMFPKSTPAPEKPNIGAGDESVARGVLGTVPVEEDGSAHFMAPAGVPFYFQ
                     ALDERGMAVQTMSSATYLHPGEHLSCAGCHEPVRQSPAVPKRLPLALRRAPDKLKAEA
                     DGSWPLQFSRLVQPVLDRHCADCHAREKEAPKFGREAGGAYGWSQAFESLRPFVWVRH
                     GGNGIGLKRNKTSYNIPGQVGAHASPLLALLEAGHYGVALSPEELRRITLWIDGNSVF
                     YGAYHDLVAQAEGQVVLPHLQ"
     gene            7774..8190
                     /locus_tag="PKN92_10645"
     CDS             7774..8190
                     /locus_tag="PKN92_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNZ49293.1"
                     /translation="MLKQFQFFAIFLCLSLLAIALLSRLPETAEENREAPAQVLRHVV
                     LFQFKDSATAAQIREIETAFCRLPSSTGLIRDFEWGTNVSPEGLDHGHTHCFILTFNS
                     EADRDSYLPHPDHKAFGELLGPCLEQVTVVDYWTKP"
     gene            8211..>8495
                     /locus_tag="PKN92_10650"
     CDS             8211..>8495
                     /locus_tag="PKN92_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49294.1"
                     /translation="MAKHPESDKAQKELMQTTAGDWERERCSRHWNLAEGMGVMCFIL
                     FALWGVAYPFGVMAGSATAKILSEVMLGLGAAFILLAAPWLHKDTASSWGL"
BASE COUNT         1880 a         2419 c         2265 g         1931 t
ORIGIN      
        1 ctgctttgtg ctgtcagtgc agcggtgttg ctccttcagg caggaatcac gcatcctgaa
       61 cgcatatcca cgcatcgggg gtggctggct gttcacggcg ggctgcatct gctgccgctg
      121 ctgtttgtga caggctgcct gcgcgatctg atcctcgcca tgcttgccgg actcaaccgc
      181 tatggctgga gagcaggagg catcacgctg gcttccctga cacaggtggc ggctgtatgg
      241 gtattcatat ggcagggagg cggcgggctc acagctctgg tgatcgcact ggcagcctct
      301 tatgcattgg cgctggcgct tctctggatc ggactgggat ctgccgccgt gccccgtttc
      361 tcacagcgcc attacagaga agccgtcttg ttcagtgctc ccttgtatct gaatcaggtc
      421 atgaatttct tccaccagcg cttcgatacg gttcttgttt ctcttctggc aggcgtaggc
      481 tatggtgctg tttatgaaat gctgaaacgc atccctctta ttgtaaaccg caccctgaat
      541 gcgctgctag tgccctatct cccccacatc tcccggctga ttgcccggga agaccgcacg
      601 caggcagcaa aggtactcaa tgaagcggca gcgctttccg ccttcatcgg ctatggtgcg
      661 gcacttttct ttactgcact gcagacaacg ctaatcccct ttctgtttaa taaggaatat
      721 cttaccggca gcacaacact ctttctgctg ctgcttgccg ccagtctggc acttcaatcc
      781 ggcgtgatgg cgcaaatgct tatcgccctg ggacgcaatc tttccgttcc gatcgtaaac
      841 atgagtacaa tggcactctg cctttttctg gatctgctgc tgatcccccg gtggggtctg
      901 gcgggagcgg cgggtgctgc cgctttttct tccggctgca gttatgcgct gatggtcttt
      961 tgggcacggt acaatggcat ccgcatctcc ctgcgcagct gtgcactgcc tctgctcttg
     1021 ctttccatca gcgctttgtc gttatattat gagatgcaga acgcactgtt ccggctgctg
     1081 ccgcctgtac tttttaccgt gctgtgtctg atgaccggtg ttacatctcc cggtcgcctc
     1141 cacgccatta caaaggcact gcttccgggc agcttctctg catcagactg atacccttta
     1201 tctactgtca ggaaaagagg agtagccggt atgacacaat caccctgtcg catcattaac
     1261 gccacggcac ccatccgcat ctgtgatctg ggcggatgga cagacacgtg gttttcaggg
     1321 cacggaagta tcctgaacat aggcgtctat ccctatgcgg aagcccagct ctttgtccgt
     1381 cccggagacc ggccgggcaa acgggaagta atgattcatg ccgagaattt cggcgagcgc
     1441 tatgccttgc ccacgccaat cgaatacgac aagcatcctc ttctggaggc atgtctggat
     1501 atcatggaac ttcccgacaa cctggatttt gaggtcaatc tcttttccat cgctccgccg
     1561 ggctgttcca caggcacttc cgccgccatc agcgtcgctt tgctgggtgc gctggatctg
     1621 cttacaccgg gacgcatgac accgcatgaa atcgctttgc ttgcccagcg tgtggaaaca
     1681 gaaaaactcg gactccagtg cggtatacag gatcagctct gttccgccta tggcggcatc
     1741 tgctacattg acatgtacga ataccccaac gcctctgtat cccagttgtg ggtacccaac
     1801 cggatctggt gggagctgga aaggcggctg gtacttgttt atctgggcag atcccattcg
     1861 tcaagtgaag tgcacgggaa agtcattgca ggttttgaag cggcgaactc cgataaaagc
     1921 gctctggaac cgcttcggcg tgcggcaaca gcagcaaaag atgcgctgta tcagggcgat
     1981 tttcttgcat tcggcaaggc gatgaaggac aacacggaag cacagcgcaa cctgcatcca
     2041 gctctgatct cggctgaagc cgaagcggtt atcgatcttg cccggaagca tggagcactg
     2101 ggctggaaga tcaacggtgc aggcggtcag ggcggctcag tcactatttt gtgcggcccg
     2161 gaaaacacag gcaagcggaa ttttattaaa gaactttcat caatcaatcc tgcctttgaa
     2221 gcacttccta tttatctgtc acgtttcgga cttcgccaat ggaaaagccc catgcaggat
     2281 cagtcttctt tttcaggatc atcatgattc ccatacactt tatttccatt gcaacgcctg
     2341 ttccctacga tgccatgctg cagcggcagg agcagatgcg tgattccgta catgcaggcg
     2401 acgacgacgc atccgttttt ctgctggagc atgagccggt aattaccttg ggcaggcgtg
     2461 ctcaggcgga acacgtccgc gccaccccgg aacagcttca cgcattggga attatcgtgc
     2521 acaagagcga ccgtggcggc gacgtcacct accacggtcc gggtcagctg gtcgtgtacc
     2581 ctgttctcga cctgaaacga tggcatcttt ctgttgactg gtacctgcga agtctggaag
     2641 aggtggtgat tcgatttctt tcctgttacg gattgcaggg caggcgcatt gacggactca
     2701 cgggcatctg gatcggcgag cagaaagtgg ctgctatcgg catcagcatc cgtcactgga
     2761 tctgctggca cgggattgcg ctgaatgttg cgccgaagga agagcactgg caaaccataa
     2821 ttccatgcgg tatacgggat cggggtatta ccagccttcg cgcccacctt gcacaggtac
     2881 cgcccatgca ggaagtcaaa aacaccatga tcacctgttt taaagaagtt tttccatgtg
     2941 cggcacgaat cagtcaggaa taacatgaaa atcggacccc gattgagaag cagtatcatt
     3001 tttctggcgg cagccctcct gctgggcatc gctttcagaa ccggaagcac acatctggct
     3061 ttcagcattt acgcttttct cctgcttctc gcaacttcct ggatgagcac tgcgctgtgg
     3121 tttccggggc tgaccagcga acggacgatc gaccggctta tggttcagca gggggaaaac
     3181 attcaggtca ctgtcaaagt gctgaacaag cgggggctgc ccgtgccgtg gcttttcgtg
     3241 gaagacccct gttcgctgcc ttttccggta cgaggcgaat caaagcggct ctgcatgctg
     3301 atgccgggac aatccgtgac cctgagctac acgcttacat gcaccagacg cggataccac
     3361 cgcatcggtc cgaccgtttt cgagtcgggc gacctgttcg ggctccataa acgctttcgt
     3421 acggcgtcca aacgggacta tgtcactgtg ctcccgacca ttgcctatat tgacacctat
     3481 gctgtttctt ccagacgtcc tcaggggccg gtccgcatta cgaaccggat ttatgacgat
     3541 ccgtcgcgta ttaacaacct gcgggaatac cagccgggcg atccgctcag caaaatccac
     3601 tggaaggcaa cggctaaaac aggcgaactg atcgtgaaga cttttgaagc gaccagtgtc
     3661 accggcggta cactggtgct ggatctgcat gaagacagct atctggaaga aacgcgggaa
     3721 tcccgcatgg aactggcaat caccaccacc gcttctattg cctacctcct tcaaatgtcg
     3781 ggcgaacaga ctggtctgct caccaacggg cgggatgcgg cagaagtcgc tctttacgag
     3841 ggcaagacac tggatgcacg aagccgcttc gagctggagg aactccgccg cctggagatg
     3901 gacagcacgc gcatcagtcc gcttaccgta cagacgcaac gcagtccgaa tcaggccata
     3961 aaaatcgcag aaaatctggc gcgcattctt cccggacagg cgctcgatct tcccgcactg
     4021 ctgacagcgc aatgcaccgg actcccccgt gatgccacgc tccttcccgt gacaggacag
     4081 atcacagacc gtctggcatt gactctgggc atgctgaagc aggcgggctt tcagatcgcc
     4141 gtctttctga tcgatgccgg aactgcccgg gaggaagcgg aaaaattact ggcacagcat
     4201 tccatttaca tgtttcatat cgcaagcgag cgggatctgc acaatatctc tctgtcgaaa
     4261 atctgagaaa ggggcagcaa caccgcaggt tgtctattgc aggtgcggca gcacgacctg
     4321 accttcagcc tgtgccacca gatcgtgata ggcaccataa aaaacgctgt tcccgtctat
     4381 ccacagagta atacggcgca gctcttcggg tgaaagtgcc acaccgtaat gccccgcttc
     4441 caggagagcc agcagaggcg aagcgtgggc acctacttga cccggaatat tataggatgt
     4501 tttattgcgc ttcagtccga tcccatttcc accgtgacgt acccagacga aaggccgaag
     4561 cgactcgaag gcctgagacc atccgtaggc acctcccgcc tcccttccga atttcggcgc
     4621 ctctttttcc cgggcatggc agtcagcaca gtgcctgtcc agtacaggct gcaccagacg
     4681 ggaaaattgg agcggccagg aaccgtccgc ttccgctttc agtttatccg gtgcccgtcg
     4741 cagggcaaga ggcagccgct tgggcactgc cggagactgt cggaccggct catggcagcc
     4801 ggcacaggac agatgttcgc ccggatgcag ataggttgcg gaactcatgg tctgcactgc
     4861 cataccccgt tcatccagtg cctgaaaata gaaaggtacc cccgccggtg ccatgaaatg
     4921 agcgcttccg tcttcctcta caggcacggt acccagcacg ccccgcgcca ccgattcatc
     4981 accggcaccg atattcggtt tttccggagc cggcgttgat ttcggaaaca tctgtatgag
     5041 gcggagctct ttaattctgg tgttttccgg ccactcccgg cgggtctcgt atatattcat
     5101 cactgaaatc acacctgtcg gcggctcagc ctgcgcaact gtttcataat gtgtctgcac
     5161 gggaataatc ggcggcctcg gccgtgcacg gaaaggaatg ggagtggaac agcctatgct
     5221 gtcatcccgg tacagaattt cccggttgcc gaaggagtcg acaagatata aatcatgctt
     5281 gctctggtca ggatcataca cagcgagata aaagtcttcg ctcagaggcc agggcgtccc
     5341 gtacacctgc ccgtcaccgc cgccgccctg aggacttcgg ggtacccccg gcgccgactc
     5401 cgcttccgga aatttagctt ccggcgttac acgtctgatc tgtcctgtgg attgatcatc
     5461 ttcaaggtga agatcaatag taatgatgga gccgaagaac tggccgtgat gcgtggcggc
     5521 agttgcagcg tatttgtgcg agccgggaat ggcacgaaga gacatttcca tccacggacg
     5581 tgattcccgg acagccgggt aattgccgtg ggggccgcgc gggtctctgc cgtccggata
     5641 gacaagccac agatgatggg caatgtcgct gtcccgatcc acgtaatccc agcgggtata
     5701 cacgaccatg ccgttattat ccacactggg cagccattca tgggtctcgt gatagctgaa
     5761 gggcagaatt tctttcaggg cactgtccat ctgaaaaagc gtataggtgg ggcacacgcg
     5821 tccgcagcgc agaaatccgc ctctccgctc agaagtaaac acaatgcggc cgtcgggaag
     5881 ccagcaggga tgcatgtcat tccagggccc gtcagtcagc tgggtcagcc ctgtgccgtc
     5941 cgcattcaca ctgaataaat gataggccct gcctggatcc cagggctctt tccagtaatt
     6001 tgcctgatct tctctgtggt acttgtggcg cttgtcgccg cggcattcca cataggcaaa
     6061 aagaatccgg gtgccgtcaa aagaaagatc cggagatagg aaggcaccgc ctcccgcatc
     6121 ggctccctcc gtcagcagcc cttcaccgtc ataataaaga tccggtgccg ccgtgccacc
     6181 gtgcagcttt tcacctgcca gccttcccga ttcaacaacg gaatcggcaa gcagatcttt
     6241 tgtgacagga gcatcggtaa acgcattttc cagaacaaat agtccgccgc cgggccgggc
     6301 ggctatgccg tagaactgat cgcacatatg ctcataaata gcacggtgtc ttttcagaaa
     6361 caggatgcga tcaaaattca gcagcggatt ggaaaaagca atctcccggc gcagtgcaca
     6421 cagacggtga aaacagagcc gctcctcctg cagcccgaca gccccctgcc cttcttcaat
     6481 cttcgcaaca aggggcgcca attcggaaag agcagcttcc atgccgctgt aatctctgtc
     6541 cggatagcgt gcggacaaat agtcaagaag tgcccgggtg cgccgccaga caatgcctgt
     6601 cggagagcgg tccgtgtcat gaatcagcga atcaggacgc agcgcttccc gggcaagccg
     6661 ctcattattc cattccggac gggcagcaat ctgccgcctc agttctctgt attcggcatc
     6721 ccgaaccgca ctgagcggaa gcgtctctct cgcaccggga aattccgcaa tgatcacgac
     6781 attatcatca aactgcggaa tatagatccg gttctcgtgc cacgccgtcc cgatatcagc
     6841 aggcgattga agcgtatgca gtgtgcggac actcttccgg tctgctgtaa tgatacaaat
     6901 tttgttgccg acaaaatccg agacgataaa gtcaccggaa ggggtgactt ctatgccgtc
     6961 caggttggtg aaatgttcgg caaggccgaa aggcaccgga gcattctgtc cggcaggatc
     7021 cacctcatag atttcatgaa gtgtccagga aacggcgtac agtttttccc cgtgaacagt
     7081 aacaccattc acgccctcaa tttctcccac aagggcatga ttgcccgccc cactgatatc
     7141 cacccgccag atgtgcccct tttcgacatc agacacataa acagcaccat cgcccgcagc
     7201 catgtcgttc agcctgcccg cccctgccgg tgcataactt tttctgaagg atccgtcggc
     7261 gagagaataa gcaacaacac gacggttgtc tgctgcataa agaactccgt tgaggattgc
     7321 catgcctttg ggctgatgca acggaaatgc ctcagtgctt tccacccatt tcaactgatc
     7381 aagttttccg tcagggctga ttcgggaaat gaagccggcc cccgtatctg tccagtatcc
     7441 ttcaggtgcc gacgcaatat tcgatacata ggcgaagcct gtttcgggat ctgcaacaac
     7501 acattccggt gtggaaaatc cgtgaatagc gtgcaaaggc tgcagttcct gtgccggaag
     7561 cccggcggac aacagaagga aacaaaggga aaaggacaaa gacaggaacg ctgaagaacg
     7621 catggccgac tctcctgaac ttgaatattt ccggatctga aaaatgaaac gatccggtat
     7681 tttggtgctc atatcactgc tgtggtattg tgtagcttct tccttttttt acgctgccat
     7741 tttaacagga acctcacgct gaaaggaaaa agcatgttaa agcagtttca gttttttgcg
     7801 atttttctct gcctgtctct tctggcaatt gcgctgctgt cgaggcttcc tgaaacagct
     7861 gaggaaaatc gggaagcacc cgcacaggtg ctgcggcatg tggttctgtt tcaattcaag
     7921 gacagtgcca cggctgcaca gatccgtgaa attgagaccg ctttttgtcg cctgccctcc
     7981 tctaccggac tgatccgtga ttttgaatgg gggacaaatg tcagtcccga agggttggat
     8041 catggtcaca cacactgctt tatcctgact tttaacagtg aagcggaccg cgacagttat
     8101 ctgccccatc ccgatcacaa agcttttggt gaactgctcg gcccctgcct tgaacaagtg
     8161 accgtagtgg attactggac aaaaccctga cctgaggaag ggactccgtc atggcaaagc
     8221 atcccgaatc ggataaagca caaaaagaac tgatgcagac aaccgccggt gactgggaac
     8281 gggagcgatg ctcacgccat tggaatcttg cggaagggat gggcgtcatg tgcttcatcc
     8341 ttttcgcctt atggggtgtt gcctatcctt tcggcgtgat ggcaggcagc gccacagcaa
     8401 aaatactttc agaagtgatg ctggggctgg gtgccgcgtt tatcctgcta gcggctccct
     8461 ggctccataa ggatacagcc tcttcctggg ggctg
//
LOCUS       DAOPWB010000129         8434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_8415_length_8434_cov_5.598759, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000129 DAOPWB010000000
VERSION     DAOPWB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8415_length_8434_cov_5.598759"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKN92_10655"
     CDS             complement(<1..288)
                     /locus_tag="PKN92_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNZ49295.1"
                     /translation="MEDQLTTRFLAQTINNRYQIISLLGSGAMGHVYLAEDSLLKRKV
                     AIKVLRGEWGEHEEVNKRLENECKLMAHLGNHQNIVALIDRLVYQNSPMIVM"
     gene            892..3606
                     /gene="topA"
                     /locus_tag="PKN92_10660"
     CDS             892..3606
                     /gene="topA"
                     /locus_tag="PKN92_10660"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006619329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNZ49296.1"
                     /translation="MIVESPAKATTIQKFLGKDYKVLASFGHVRDLPKSASEIPVAHK
                     SKPWARLGVDVDADFHAIYVVQRDSAKQINELKKAAKEAEEIILATDEDREGESISWH
                     LLEVLKPRVPIKRIAFHEITKSAIDAAMRSPRDINQPMVRAQETRRILDRLFGYELSP
                     VLWRKVGSKLSAGRVQSVALRLVVEREEDRRRFVRAGYWDAEAVLNRDGKQFKATLIS
                     LNGCRIATGKDFDDVTGALLKKGSGAPVWLKEEEARALVPTLQKAVPWKVASVEEKES
                     QLHPSPPFITSSLQQAASTQLGFSPRKTMQVAQKLYEGVDLGDGNREGLITYMRTDSV
                     ILSEKALQDASAYIHKHFGEAYHKRRQFTTKSKMAQEAHEAIRPTDVFKTPASLSSRL
                     GKDEFRLYQLIWNRTLASQMAAAQVMRTTVDIAAAAPGSEALFRATGAVVVFPGFLEV
                     MNDRQRENELPPLAEGMKIGADSNSDVLLESLEALQHETQPPARYTEASLVRRLEEEG
                     IGRPSTYAPTVAVIQQRGYVSRCGTALAPTYLGIAVVMLLRKYFSTYVDLGFTAQMED
                     ILDAIAGGEEDWLAFLRRFYHGEPASNTVGLKPDIDAKLEMIEYPAIPLGTTEAGEPV
                     VVRLGKTTPFIQRGAGGDGNTISLPDQLFYDELTLGKALELLENQAQRDESLGICPDT
                     GKPILLLEGPFGPYVQLGEAEKGTKPKRTGLPKGMALESCDLEKALYLLSLPRELGLH
                     PETKKAIVLGIGRFGPYVVHDGDFRSIKADDFFSVTFETALALLQTPKASAAAKKLIR
                     TFEKQAETDPEISLYQGRYGPYVTDGSVNASLPAGTDLDGLTLEQALALIAVAAERKK
                     NAPPRKASAKKPAAKKTAAKKAAVKKAPAKKAAAKKPAVKKATTKKEQ"
     gene            3635..4432
                     /gene="thiD"
                     /locus_tag="PKN92_10665"
     CDS             3635..4432
                     /gene="thiD"
                     /locus_tag="PKN92_10665"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNZ49297.1"
                     /translation="MKRVLSIAGSDSGGGAGIQADLKTCAALECYGMSAITATTAQNT
                     LAVTDIHEIPPSHVEAQIRAVLDDIGADAIKTGMLSSASIIDSVASALESYSLPPVVV
                     DPVMISKSGAHLLRPEAVETMKRRMIPLATLITPNIPEALELTGLSLESDSRIPSILE
                     ALKALGCACVLLKGGHAESADSTDYLFDGNSIVSYPAPRIDTCHTHGTGCTLSTAIAC
                     FLARGETMKDAVQSAKDYLTGAIAHSETLRVGKGTGPVHHGWREGRL"
     gene            4667..5917
                     /locus_tag="PKN92_10670"
     CDS             4667..5917
                     /locus_tag="PKN92_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49298.1"
                     /translation="MYDKEALEEFTTACGLKRYRVPVSDPALNRHRFISSDTPEFTLH
                     KAALQEKLWVALAEQRAEEKRRRLGLEMNGKRSDLTARQKQKIAEDRTVMAREEFSHT
                     FNTLEYGLHHPLDFNWDFFRTSMPFSVPRPSVSAGKEPTPPIYPREPQRTDFAYTPKY
                     EAMDVLRPGRKQQKDKEAQERFREAHKHWQRLCGRMKYIYDEQMADHTRKLRQATENL
                     PKELAAWEQAKAEYEQIQEMSMSIVQAKEEALAQHDPHAVLDYFDMALATSSYPAAFP
                     HSFEMDYKPATQRLIVDYLLPSLKALPRLVTVVYNEVTDSFSDQIITVEQQAALYTKL
                     FYEIPLRTFQELFSLDTSDALQSIRFRGYLFLEQAGAERTNLPPTCIVDVKAERSVFT
                     GIGLRESDAISTFEKLGGVIHSLV"
     gene            5933..8353
                     /locus_tag="PKN92_10675"
     CDS             5933..8353
                     /locus_tag="PKN92_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ49299.1"
                     /translation="MPGNDKRHPRLSRLRSGALLLLLMGLVSVVGFSCLSTWKGPAGS
                     LTNDLFVPSILLNAGLGFSNADADTVPGLRDFLDFKSTAFSLENIKAGTTFQPLHPYQ
                     EFHRYLLYTLALVWYCFGINWISVKLLLIALLVLSALLTYGISRLVLNRLLSFLVALS
                     FSASAGVYWTLPVLRDFAKVPFILALLLLMGLFIRRRMTPRLYWLLALSAGAVMGIGM
                     GFRRDLLVFLPPVLFFIAAARLQHTSPSALFQRAGALLLAFGAFVLAGLPVHLSLCRD
                     GYVAAHDTLMGFATYSDQELALLRSGSYEKHALLNDLFSTYKAYDAAKRGVTFRKGVY
                     EERLSDPHFDLEMKSAYARKIMLTFPADMLLRAYASVLRINTGSAPAPYYPLRLIEKY
                     GFLFLIVALLLSSAVKPRQTFLLLLLVLYCCGITSLQFGVRHSVHLAFLPYFFAAMTI
                     QLMISAAVQFRKIRCGNASFPSFTAVLRRRWHYVPVLLLLLLLFWLPVKISGMLQERQ
                     FRPLLRHYEAQESRPLRYIARQWDDRMIFMPLRDRVHEKSESFHLIDHVSTSFLEARF
                     QQVREPLDIRFVYEADQDFWDFSAPLACEVAEDALPCALRLFFPVPELKGEDAASHFT
                     GLSLPMSQRQYFAGFQEIETPETGELLMPVLIPEKEGSFLFRQRLELPWAGTWWRQYP
                     KEDVFDAAIAKEGIKHAIMTGRAEEAVEKAQDALSHRPDSPAFSLLYMEALEESGRAQ
                     DARMVAQHLLRSFPGARALYARLGSFYATRGGADRQRQEWQCLPAQLADRRFEDEFCY
                     LTARPQQE"
BASE COUNT         1906 a         2154 c         2322 g         2052 t
ORIGIN      
        1 catcactatc atgggggagt tctggtagac aagccggtcg atgagggcaa cgatattctg
       61 atgattgccc agatgggcca tcagtttgca ctcattttcc aggcgtttgt ttacctcttc
      121 gtgctcgccc cactcgccgc ggagcacttt aatggcgacc ttacgcttaa gcagcgagtc
      181 ttcggcaaga tagacgtgcc ccatagcacc ggagcctagc agcgaaatga tctggtaccg
      241 gttattgatg gtttgagcga gaaagcgggt tgttagttga tcttccatag gagccttcct
      301 gtgttattgg atagctttaa aagaagaata aaaaaacgga ctgctatatg cgttggattt
      361 cattttaacc tgctttaaaa tgaacattgt ttgcattata gcatgaggga catgtctctt
      421 tgcatttaaa caccctttat gagaatgttt tttagcactg cggtttattt ttgcctgatt
      481 tgttccacga attttctgta aaaaaaagaa agtttattcc tgcggaaaag gaactgttca
      541 ggtgaagtcc ggcgcaggca tctgaaataa agccggaatg tataacgttt ggattcatca
      601 gaaaaacaga tgccgatgca gctccatctt caaggagtta aaccatattt caacctacgg
      661 gcagatcctt tggagggtgc actctgcaaa aacaaagcgt cgggtctttt gtttttttgt
      721 ttaaaaataa ggtatagtcc caatcctgca gcgcttttca ggcacaaaaa tataaaataa
      781 gtatagtaaa agcacggaaa gcgctgtgct tcaccttata ggaagcaacg tctcgtccgc
      841 cgcttccctt ttttaaccct ttaacggaca ggtatatcaa tcttgaagct cgtgatagta
      901 gagtcgcctg caaaagcgac aacaatccag aaatttctcg gcaaggatta caaggtgctt
      961 gccagttttg gtcatgtccg tgatttgccg aaatcagcga gtgaaattcc cgtcgcacat
     1021 aaaagtaagc cctgggcacg gctaggcgta gacgtggacg cggactttca tgccatttat
     1081 gtggtgcagc gtgatagcgc caagcagatc aatgagctga aaaaggcggc aaaagaagcc
     1141 gaagaaatta ttctggcaac ggatgaagac cgggagggcg agtcgatcag ctggcatttg
     1201 cttgaggtgc tgaagccccg ggtaccgatc aaacgcattg cttttcatga aatcaccaaa
     1261 agcgctattg atgccgctat gcgttcaccc cgcgacatta accagcctat ggtgcgggct
     1321 caggagacgc ggcgcatcct tgaccgccta ttcggatacg aactgtctcc cgtgttgtgg
     1381 cggaaagtgg ggagcaaact gagtgccggg cgtgtgcaaa gtgtagcact gcggctggtt
     1441 gtggaacgtg aagaggatcg ccggcgtttt gtgcgtgcag gatactggga tgcggaagcg
     1501 gttctgaatc gcgacggcaa gcagttcaaa gcaactctga tcagcctgaa cggttgccgt
     1561 atcgccacag gcaaagattt tgatgatgtt acgggagcgc ttcttaaaaa aggaagcggt
     1621 gctcctgtgt ggctgaaaga ggaagaggcg cgtgcactgg tgccaacgct gcagaaagcc
     1681 gttccgtgga aagtggcgtc tgtcgaggaa aaggagagcc agctccatcc gtcaccgcct
     1741 tttattactt cttcgctcca gcaggcagca agcactcagc tgggattttc tccgcgcaag
     1801 accatgcagg tagcgcaaaa gctttacgaa ggggtggatc tgggcgacgg aaaccgtgaa
     1861 ggtctgatta cctatatgcg tacggactcg gtcattctca gcgaaaaagc gctgcaggat
     1921 gcctcggcgt atattcataa acacttcggc gaggcctatc acaaaagacg gcagtttaca
     1981 acaaaatcca aaatggcgca ggaagctcac gaggcgatca gacctacaga tgtttttaaa
     2041 acacctgctt ccttgagcag cagacttgga aaagatgaat ttcgtctcta tcagctgatt
     2101 tggaaccgca ccctggcctc tcagatggcg gctgctcagg tgatgcgcac gaccgttgat
     2161 attgcagcgg ctgcgcccgg atcggaagcc ctgtttcggg caaccggtgc tgtagtcgtt
     2221 ttccccggct ttctggaagt gatgaatgac aggcagcgcg aaaatgagct gccgcctctg
     2281 gctgaaggca tgaaaatcgg cgccgacagc aacagcgacg tgcttctgga atcactggaa
     2341 gcattacagc atgaaacgca gccgcccgcc cgctatacag aggcatctct ggtgcggcgg
     2401 ctggaagagg aaggcatcgg gcgcccttcc acctatgcgc ccactgtcgc cgtgatccag
     2461 cagcgcggtt atgtgtcacg gtgcggtaca gcgctggcac ccacctacct gggtatcgct
     2521 gtggtgatgc ttctgcgcaa gtacttttcc acctatgtgg atctcggttt tacagcgcag
     2581 atggaagaca ttcttgatgc aattgccggt ggtgaagagg actggcttgc ctttctgcgc
     2641 cgcttttacc atggagaacc cgccagtaat acagtcggtt taaaaccgga cattgacgcc
     2701 aaactggaaa tgattgaata tcccgccata ccgctgggta caaccgaggc gggcgagccg
     2761 gttgtcgtgc gcctgggcaa gacaacgccc ttcatacagc gcggtgcggg cggcgacgga
     2821 aacaccatca gtctcccgga tcagttgttt tatgatgagc tcactctggg aaaagcgctt
     2881 gaacttcttg agaaccaggc gcagcgggat gaatccctgg ggatttgtcc ggataccggg
     2941 aagccgattc tcctgcttga aggtcccttc ggaccctatg tgcagcttgg tgaagcagag
     3001 aaaggcacga agcccaagcg taccggcctg cctaaaggaa tggcgcttga aagctgtgat
     3061 ctggaaaaag ccctttatct gctctcgttg ccccgggaat tagggcttca tccggaaacg
     3121 aagaaggcca ttgtgctggg tatcggcaga ttcgggcctt atgtggtgca tgacggcgac
     3181 ttccgcagta tcaaggcgga tgatttcttt tcagtcactt ttgagactgc tttagcactt
     3241 ctgcagacac ccaaagcctc tgcagccgcc aaaaaactga tccgcacctt tgaaaaacag
     3301 gcggaaacag atcccgagat cagcctttat cagggacgtt atggccccta tgtgacagac
     3361 ggcagtgtga acgcctcttt gcctgccggt acggatctcg acggcctgac gctggaacag
     3421 gcgcttgcct tgatcgccgt ggcagcagag cgcaagaaaa atgcgccacc cagaaaagcg
     3481 tcagcgaaga aaccggcagc caaaaaaact gcggcgaaga aagcagctgt gaagaaagct
     3541 cccgctaaaa aagcggcggc gaagaaaccg gcggtgaaga aagccacaac gaaaaaagag
     3601 cagtgaaggt ctgtcgctgc gtcggagttg aagcatgaag cgtgttttat ccatcgccgg
     3661 gagtgacagc ggcggcggtg ccggtattca ggcggacttg aaaacgtgtg ctgcgctgga
     3721 gtgctacggc atgtccgcta tcacggcaac aacggcgcaa aacacgcttg ctgtgacgga
     3781 tattcacgag atccccccat ctcatgtgga ggcacagatt cgtgccgtcc tcgatgacat
     3841 cggcgctgat gccatcaaaa cagggatgct ctccagtgct tccattattg acagcgtcgc
     3901 atcggcgctg gaaagttatt cactgccccc tgtggtggtg gatcctgtga tgatttcgaa
     3961 aagcggtgcc catttgctgc gcccggaagc ggttgaaacg atgaaacggc gcatgatccc
     4021 tctggcaaca ctgatcacac ccaatattcc ggaagcactg gaacttaccg gtctgtctct
     4081 ggagagcgat tcccggattc cctctattct ggaggcattg aaagcccttg ggtgcgcctg
     4141 tgtgctcctg aaaggcggcc atgcggaatc ggcggactcc accgattatc tctttgacgg
     4201 aaacagcatc gtttcctatc ctgcaccccg gattgacacc tgccataccc atggcaccgg
     4261 gtgcacgctg tccactgcca tcgcatgttt tcttgcccgg ggcgaaacaa tgaaagatgc
     4321 ggttcagtcc gccaaggact acctgaccgg ggcaatcgcc cacagtgaaa cgcttcgtgt
     4381 tggcaagggt acgggcccgg tacatcatgg ctggcgtgaa gggcggctgt aacagtcctg
     4441 agcggggagc agcgctgcac tgagaagaaa aagcgctgcc gcctcgattt tctgtgacag
     4501 aaaaaaaagt gccttctgct tgtttttatc ttttcggctc gttacaatgt ataggcaagt
     4561 gctgacaaag cggctgcagg ataaaaagag ttaagttccg aatgtcaccg gaaactgtgg
     4621 gagctcagga cttgcgctca acagggtttc agttgcgaga gtctagatgt acgacaaaga
     4681 ggcattagaa gaatttacga cagcctgcgg acttaagcgc tatcgtgttc ccgtgtctga
     4741 tcctgcgttg aaccggcatc gcttcatttc ttcggacacc cctgagttta cactccataa
     4801 ggcagccctg caggaaaaac tctgggttgc tttggcggag cagcgtgcag aagaaaaacg
     4861 ccgtcgcctc ggccttgaaa tgaacgggaa gcgatccgac cttactgcgc gtcagaagca
     4921 gaaaatagcg gaagatcgta cagtgatggc gcgggaggag ttttcgcaca cctttaacac
     4981 gctggaatac gggctgcacc atccactgga tttcaattgg gattttttcc gcacctccat
     5041 gcctttttcc gtaccccgcc cgtccgtgtc tgccggcaaa gagcctacac ctcccatcta
     5101 tccgcgtgag ccacaaagaa ccgacttcgc ctatacgccg aaatatgagg ctatggatgt
     5161 gctgcgcccg ggaagaaagc agcagaagga taaagaggcg caggagcgct ttcgggaggc
     5221 gcataaacac tggcagcgtc tttgtggccg catgaaatat atctatgatg agcagatggc
     5281 ggatcatacc agaaagctgc gccaggctac ggaaaacctc ccgaaagaac ttgccgcctg
     5341 ggaacaggca aaagcggagt acgagcagat tcaggaaatg tccatgagta ttgtgcaggc
     5401 taaagaagaa gccctcgcac agcatgatcc gcatgccgtt cttgattatt tcgatatggc
     5461 gctggcgacc tcgtcctatc ctgccgcttt tccccattcc tttgagatgg attataaacc
     5521 ggcaacacag cggctgattg tggattatct gctcccctct ctcaaagcgc tgccccgact
     5581 ggtcacggtg gtttacaatg aagtgacaga cagtttcagt gatcagatca ttactgttga
     5641 acaacaggcc gcactttaca caaaactgtt ttatgaaatt cctcttcgta cttttcagga
     5701 acttttttcg ctggatacct cagatgcgct tcagtccatc cgtttccggg gctatctgtt
     5761 tctggagcag gcaggggcgg aaaggaccaa tcttccaccg acgtgtattg tggatgttaa
     5821 agcggagcgc tctgttttta ccggcatagg tctgcgtgag agtgacgcaa tttcgacctt
     5881 tgaaaagctg ggcggcgtta tccattcact ggtttgaccg gtgtctcttc ctgtgccagg
     5941 caatgataaa cgccatcccc gcctgtcccg gctccggagc ggagcgctgc ttctcctcct
     6001 gatgggtctg gtttctgtgg tcgggttttc ctgtcttagc acctggaagg gacctgccgg
     6061 ttcgctgacc aatgaccttt ttgttccgtc aatactttta aatgccgggc tgggtttcag
     6121 caatgccgat gcggatacag ttccggggct ccgagatttt ctcgatttta aaagcactgc
     6181 cttttctctc gaaaacatca aagcaggcac aacgtttcag ccgctgcatc cctatcagga
     6241 atttcaccgg tatcttcttt atacgctcgc gcttgtgtgg tactgcttcg gtatcaactg
     6301 gatttcggtg aagctgctgc tcattgcgct gctcgtgctg agtgcgctgc tgacgtacgg
     6361 gataagcagg cttgtcctga accggctgct cagctttctg gttgcgctgt ctttttccgc
     6421 ctccgccgga gtgtactgga cactgccggt tcttcgtgat tttgccaaag tgccttttat
     6481 tctggcgctg ctgctgctga tgggactctt catacgtcga cgtatgactc cccgtcttta
     6541 ctggcttctt gctttaagtg caggcgcagt gatgggtatc ggtatggggt ttcgccggga
     6601 tctgctggtt tttctgccgc cggtactttt ttttatcgcc gcggcgaggc tgcagcacac
     6661 ttcgccttct gccttatttc agcgggcggg ggcactgctt ctggcttttg gtgcttttgt
     6721 attggcggga cttccggtgc atctctccct ctgccgtgat ggctatgtgg cagcccacga
     6781 tacgctgatg ggctttgcca cttattccga ccaggaactg gcgctgctcc ggtcgggttc
     6841 ctatgagaag catgcgctgc tgaatgacct tttcagcacc tataaagcct atgatgctgc
     6901 gaaaagaggt gttactttcc gtaaaggtgt ttatgaggag cggctgagcg atccgcactt
     6961 tgatctggag atgaaaagcg cttatgcgcg taaaatcatg ctcacctttc ctgccgatat
     7021 gcttctgcgc gcctatgcct ctgtgctccg tatcaataca ggctccgcac cggcgcccta
     7081 ttacccgctg cgcctgatag aaaaatacgg attcctcttt ctgattgtcg ctttgctgct
     7141 ttcgtccgct gtaaagccgc gccagacttt tttgctgctg ctgctcgtat tgtattgctg
     7201 cggtatcacc agcctgcagt tcggtgtacg ccacagcgta catctggcgt ttcttccgta
     7261 tttttttgcg gccatgacga tacaactgat gatctccgct gccgtgcagt tccggaaaat
     7321 ccgctgcgga aacgcctctt ttcccagttt tacggctgtt ttgcgcaggc gatggcatta
     7381 tgtccctgtg cttcttttgc tgctgcttct gttctggctt cccgtcaaaa tcagcggaat
     7441 gctgcaggaa aggcaattcc gtccgctgct ccgtcactat gaggcgcagg aaagcaggcc
     7501 ccttcgctat attgcccggc aatgggatga ccgcatgatc tttatgcccc tccgtgaccg
     7561 ggtgcacgaa aagagtgaaa gttttcatct gatagatcat gtctccactt cttttctgga
     7621 ggcgcgcttt cagcaagtcc gggagccgct ggatatccgt tttgtttacg aggctgatca
     7681 ggatttctgg gatttcagtg cgccccttgc ctgcgaagtg gcggaggatg cgctgccctg
     7741 tgccctgcgg cttttttttc cggtgcctga actgaagggg gaagacgctg ccagtcattt
     7801 tacaggtctt tcattgccga tgagccagcg ccagtatttt gcagggtttc aggagattga
     7861 gacgccggaa acaggcgagc tcctgatgcc ggtgctgatt cctgaaaagg aaggatcttt
     7921 tcttttccgc cagcggctgg agcttccctg ggccggtacc tggtggcggc agtatccgaa
     7981 agaggatgta tttgacgctg ccattgcgaa agaggggatc aaacatgcca taatgacggg
     8041 tcgggcggaa gaagccgttg aaaaagcgca ggatgcgctg agccatcgtc cggacagtcc
     8101 tgctttcagc ctgctttaca tggaagcatt ggaagagagc gggagagcgc aggatgcgcg
     8161 catggtggcg cagcatcttc tccgcagctt ccccggcgcc cgcgcccttt atgcacggct
     8221 cggcagcttt tatgccaccc ggggcggtgc tgatcggcag cgtcaggaat ggcagtgcct
     8281 tccggcgcag ctggcggatc gtcggtttga agatgagttc tgctatctta ccgcccgtcc
     8341 gcagcaggaa taaagaacgg agagaaaacg ctttcctttt cggcggattc aaggctgaaa
     8401 tgcaattatt gaattgaata agacttcggc aggc
//
LOCUS       DAOPWB010000130         8335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_8544_length_8335_cov_4.781643, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000130 DAOPWB010000000
VERSION     DAOPWB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8544_length_8335_cov_4.781643"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKN92_10680"
     CDS             complement(<1..375)
                     /locus_tag="PKN92_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNZ49300.1"
                     /translation="MFVDRVRVKVKSGNGGNGCCSFRREAFVPRGGPNGGDGGNGGNI
                     YFRASARLTTLVDMRYHPNWAGKRGMHGQGKNCTGRNGEDLYLEVPVGTVVQDYETGE
                     VCADLTKDGQEFLAARGGQGGRG"
     gene            complement(474..737)
                     /gene="rpmA"
                     /locus_tag="PKN92_10685"
     CDS             complement(474..737)
                     /gene="rpmA"
                     /locus_tag="PKN92_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNZ49301.1"
                     /translation="MASKKAGGSSRNGRDSHSQRLGVKRYGGQSVRAGNIIVRQRGTR
                     LHPGVNCGVGNDHTLFALVDGVVSFRYFKRNRKCVDIIPATAE"
     gene            complement(750..1061)
                     /gene="rplU"
                     /locus_tag="PKN92_10690"
     CDS             complement(750..1061)
                     /gene="rplU"
                     /locus_tag="PKN92_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNZ49302.1"
                     /translation="MYAVVKTGGKQYKVAANSRIRVEKLDYAVNDIVELDSVQMVITE
                     DKIIFEPDALKSARVLARVEEQGRGKKIRVYKKKRRKGYERVQGHRQSYTLLHIQEIK
                     A"
     gene            complement(1108..1920)
                     /locus_tag="PKN92_10695"
     CDS             complement(1108..1920)
                     /locus_tag="PKN92_10695"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HNZ49303.1"
                     /translation="MGMMKIGLVGCGNIASDICRAVTEKELAATVVAVNDIDATQAET
                     LLRRYKLNASILDLEKLADTADFIVECAVGAVVPAVIEAAIRFGKDCLIMSLGGLLGN
                     PALIDQARGAGIRLRLPSGAVCGLDGIRAALQGGLSEVTLTTRKPPQGLKGAPYLLEH
                     GISVENLLEALVVFEGSARDAVKAFPANVNVAAALSLAGIGADKTQVRLIADPAATVN
                     SHEIRATGAFGEMVTIMRNQPSPRNPKSSYMASLSAIAEVAAAAAEFVSGAE"
     gene            complement(2105..3805)
                     /locus_tag="PKN92_10700"
     CDS             complement(2105..3805)
                     /locus_tag="PKN92_10700"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ49304.1"
                     /translation="MISVLIAVVIVSGIVLLLSLYMTLLDSLYPEQLRTNEVLHLRTH
                     DLWMLRLCRYRKSRASGEPVLFVHGLGANQHNFTNPENGCLVDYLSSKGYDCWTVDLR
                     GTASSRPPFERSRNEVLLEDFFTEDLPAVIGHILKTTNYSKLHWVGHSMGGMLLYAYV
                     QTHGSDQIASAVALGAPLDFSDAGKAVPTWLVKFGIRFPAVAGQLVRGSIPLLRLLRL
                     SPPAFPLNRRNIPKRMHAGHFISMIEDPLPGLLRQVLRWMHGEKYVLCDGTLDIVKNL
                     PSFPIPLLAFYGYKDPFVGYDRAKKVFDSILLEDKKAILCAKAQGFAEDYSHCDLAFS
                     REAESEIFEPVARWLAEHSCVKQQENGADPHGVAAAVVSPEKRAHMLSGAAYSHLHAA
                     PAMQEGASVKKSPQEEDAASESTKTDFLKSGDTPNQYPSDDLKRRVSRVVVTRSASSK
                     EPDAPPKASAPKTAPPAGKKAAAEQREGSEKKDKSPVKGKKKSAPQKKVDMRPATAKA
                     RKAGAAPPKSVSSVAATIGGIEISPEALARAESIRAARNESLSQLLSDLDKDPGSGKS
                     "
     gene            complement(3908..5770)
                     /gene="uvrC"
                     /locus_tag="PKN92_10705"
     CDS             complement(3908..5770)
                     /gene="uvrC"
                     /locus_tag="PKN92_10705"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNZ49305.1"
                     /translation="MKDKPENRNAPEKKEKPPAISAADAPTEPGCYLMRDEDGVVLYV
                     GKAINLRNRIRSYIQLTDSRYSVKFLMRRVASVEYLTVSSEKEALLLENTLIKLHKPR
                     YNVRLRDDKTFISLRIHPGERFPRLTVVRRRKKDGARYFGPYHDTKAARKTLKQLQKL
                     VPLRTCSDSVLNNRSRPCIYYQIKQCKAPCVGFINEEEYAGLVEQVLMILEGKSRSLE
                     KQLREEITALAEQLRFEEAAVLRDRLVDLQTTLQPQHTVALEGTEEQDVFGMYAEGRF
                     VEFQVLYYRNNAMTGGDTFSFDHVEVPLNELFSSFILQFYEATPVVPKTVVLPVELED
                     QETLTEILSEKRERAVTLTVPKRGRLKELVKLAEHNAEASFISRRGREKALQDGLEQI
                     AALFKLPAAPTRIECFDISTHQGDKTVAAMSVLTHGEPDKSRYRRYQIKSQVTQDDFN
                     AMREVLTRRFSRAIREADLPDLVLIDGGKGHLNIALSVLRELGLSSLPCVSIAEGRTE
                     KGAVLPERFFLPGRSNPLVPPQGSQVVRMLAGLRNEAHRFAITYHRKKRSGAVLKSAL
                     SDIPGVGTARARALLETFGSVKNIMEATSDQLARVPGISAKLAEVVHRHLHAEG"
     gene            complement(5951..7264)
                     /locus_tag="PKN92_10710"
     CDS             complement(5951..7264)
                     /locus_tag="PKN92_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49306.1"
                     /translation="MKTFMRQRKYTVAALAVQCFLIAPAVRALDEEAILAAFRDSYGW
                     LGSAAMHISSVGEKIVPEGKNDTETRQKLRGYEDVNFFIDDAKGWHASWDSRTIDVAT
                     GKVARSESGSLRQFSRESGLFKEAGNWAVEYGYDEEGGRYLDLDPKLDGRASLDASFG
                     CTAVVTGHSLSLSHLRIPELLESYAFQVSDGELGGAASCIVESDTEYGQIKLWLSPTE
                     NYSLQKAVFSKEYGKDLGWRGDKVFAKDYQGTIPSHYVQEIHVLKKEARGAYYIPAEV
                     EVLTYSETGGTMRDGWKERITVSDVILNPDFAALKGFVLQVPQESCIGITNRSDMLFH
                     TASWKEGKPAFHLEDEDLRTRGQLFLSTIEITKTSFVDKALRWLNNKLRKQDTGMYYL
                     KNAFAFNIQTVLLLVLTVLASCWLLWRFITRLRKGFGKESCSAGI"
     gene            7706..>8335
                     /locus_tag="PKN92_10715"
     CDS             7706..>8335
                     /locus_tag="PKN92_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNZ49307.1"
                     /translation="MFENLAKRLDSVFKTIRGQGRLTEANMRDAMQQIRTALLEADVN
                     YQVVKKFIAEVQEAALGQEVLASIHPGQQIVKIVHDTLVRTMGEKNEPLNRASKGPTV
                     IMLCGLQGQGKTTSAGKLAVHLKKSGHKTLLVGADVYRPAAIKQLEIVAKQAQAEMFS
                     LGEHADPVETCLMARGEASLRGCDTIILGTAGRLHVDEEMMLEARQIAEQ"
BASE COUNT         1835 a         2259 c         2165 g         2076 t
ORIGIN      
        1 ccccctgccg ccctgtccgc cccgtgccgc cagaaactcc tgtccgtctt ttgtcagatc
       61 tgcacacact tcgcctgttt catagtcctg gacaacagta ccgaccggca cttcaagata
      121 aagatcttcg ccgttgcgac cggtgcaatt ctttccctgc ccgtgcatgc cccgctttcc
      181 cgcccagttc ggatgatacc gcatgtcaac aagcgtagtc agtcgggcgg aggcgcggaa
      241 ataaatattg ccgccgttgc cgccatcgcc gccgttgggt ccgccccggg gcacaaaggc
      301 ttcccggcgg aaactgcagc agccattgcc gccgttgccg ctttttactt ttaccctgac
      361 acgatcgacg aacatggaga tcaccttttt ttcagtcgga cttcatataa aaacagacac
      421 ccccaaagtt tcaggggtgt ctggatttct cggatgcaac gatgctgcgg attttattcc
      481 gccgtggcgg ggatgatgtc cacgcatttg cgattgcgct tgaagtagcg gaaagataca
      541 acgccgtcca caagagcaaa aagagtgtga tcattgccca cgccgcaatt gacgccggga
      601 tgcagacggg tgccccgctg acgcacaatg atatttcctg cccggacgct ctgaccgccg
      661 tagcgcttta cacccagacg ctgtgaatga ctgtctcgac cgtttcgcga actgccgccg
      721 gctttcttcg atgccattgt agtttctcct tatgccttga tctcttgaat atggagaagg
      781 gtataacttt gccggtgacc ttgcacccgc tcatagcctt tccgccgttt cttcttataa
      841 acacgaatct ttttgccgcg accctgctct tccacacggg caagcacccg ggcggacttc
      901 agtgcatcgg gctcaaaaat aattttgtct tcggtgatga ccatctgcac agaatcaagc
      961 tctacgatgt cattgacagc gtagtccagc ttttccacgc ggatgcggga attggcagcc
     1021 actttgtact gcttaccgcc tgtttttacc actgcgtaca tatctatctc tccaaagtaa
     1081 acttcgcttc gctgcgaaac ccgggggtta ttctgccccg gacacaaatt ctgccgctgc
     1141 cgctgccact tcagcgattg cggataaaga agccatataa ctcgattttg gattgcgcgg
     1201 ggacggttga ttgcgcatga tggtgaccat ttcgccaaag gcgcctgtcg cacggatttc
     1261 atggctgttt actgttgccg ccggatctgc aatcagacga acctgcgttt tgtcggcgcc
     1321 tatgcctgcc agactcagtg cagccgcaac attcacattt gccggaaaag ctttcaccgc
     1381 atcacgggcg ctgccttcaa agacaaccag ggcctccaat aaattctcta cggagatgcc
     1441 gtgttcgaga agatagggcg ctccctttaa tccctgtggc ggttttcggg tggtcaatgt
     1501 tacttcactg agccccccct gcagtgcagc ccgtattccg tccaatccgc atacagcgcc
     1561 ggacggaagg cgcagccgga ttccggcacc gcgtgcctga tcaatcagtg caggattgcc
     1621 aagcaatccg cccaggctca taatcaggca gtctttgccg aatcggatcg cagcctctat
     1681 gactgccggt accacagcac ccaccgcaca ctccacgata aagtccgcag tgtccgccag
     1741 cttttccaga tccaaaatcg aagcattcaa cttatatcgg cgcagcaggg tttccgcttg
     1801 cgtcgcatca atatcattaa ccgctactac agtggcggca agttcttttt cagtgactgc
     1861 acgacagata tccgaggcga tattgccgca cccgacaaga ccaatcttca tcatgcccat
     1921 ggggctttat tatgccttat gcacaccttg tccgaaagcg caagggtaat ggggaaaaat
     1981 caggtatgcg aagattttgt agagaaaaaa ccgccgcccc ggcgatacgt gcctcgtcgg
     2041 ttcgtgtagc atagcaaaaa cagcaggcgt gtgtcaattt tgagcactgc ctttgcccgg
     2101 gcactcagga tttcccggaa cccggatcct tatccagatc ggataataat tgtgacaggc
     2161 tttcgttgcg ggcagcgcgg attgactccg cacgcgcgag cgcttccggg ctgatttcta
     2221 tgccgccgat ggttgctgcg acggacgata ccgattttgg cggtgcagcg cctgccttgc
     2281 gtgctttggc ggttgccggg cgcatgtcca cttttttctg cggtgcagat ttcttttttc
     2341 cttttaccgg gcttttgtct tttttttctg aaccttcccg ctgctctgct gccgcctttt
     2401 ttcccgccgg tggcgcggtt ttcggagcag aggccttcgg gggagcatcc ggctcttttg
     2461 agctggcgga tcgggtcaca acgacacggg aaacccgacg ttttaaatca tcagaggggt
     2521 actgatttgg tgtatccccg cttttcaaaa agtcagtttt tgtggattca gatgccgcat
     2581 cttcctcttg cggactcttt ttcaccgatg ccccttcctg catcgccggg gcggcatgca
     2641 aatgagaata ggcagctcct gaaagcatgt gtgctctctt ttcggggctg accacggcgg
     2701 cggcaacgcc gtgcggatca gcaccatttt cctgctgctt tacgcaggag tgttctgcca
     2761 gccaccgggc gacaggttca aagatttcgc tttccgcctc cctggaaaaa gccaggtcgc
     2821 aatgactgta atcttccgca aagccctgcg ctttggcgca gagaatggct ttcttgtctt
     2881 ccagcagaat ggagtcgaaa acttttttgg ctctgtcata tcctacaaac ggatctttat
     2941 atccgtaaaa agcaagcagc gggatcggaa aagagggaag attcttcaca atatcaagtg
     3001 tgccgtcgca aagcacatat ttttcgccat gcatccaccg cagtacctga cggagcaaac
     3061 cgggcagggg gtcttctatc atgctgatga aatgccctgc atgcattcgt ttggggatat
     3121 tccggcggtt caacgggaaa gccgggggag agagtctgag cagtctcagg agcgggatgc
     3181 tgccgcgtac cagttgccca gcgacggctg gaaatcggat gccgaatttg acgagccagg
     3241 tcggaacggc tttccctgcg tcactgaagt ccagtggtgc gcccagggcc acagcggagg
     3301 caatctgatc acttccatgg gtctgcacat atgcgtagag gagcatgccg cccatggaat
     3361 gccctaccca gtgcagtttg ctgtaattgg tcgtcttcag gatatgcccg ataacagcgg
     3421 gaagatcctc cgtgaaaaaa tcttccagaa gcacctcgtt gcgcgagcgt tcaaaaggcg
     3481 gacgggagga agcagtgccc cgcagatcca cagtccagca atcgtagcct ttactactga
     3541 ggtaatcgac gaggcatccg ttttcaggat tggtgaagtt atgctgattg gcgcccagac
     3601 cgtggacaaa tagaacgggc tctcctgagg cgcggctttt ccggtaacgg caaagacgca
     3661 gcatccacag atcgtgtgtg cggaggtgga gtacttcatt ggtgcgcagc tgctcaggat
     3721 agagtgaatc cagcaatgtc atgtaaagag aaaggagcag gactatgccg gaaacgatca
     3781 ccaccgcaat caatacactg atcatctttt ttcctccacg ccggggcgga cagagcccga
     3841 tgcataacgg aaccggataa aaaccatgtg ctccgatttt tgccggatca ttggcagctg
     3901 ccgcggctca gccttccgca tgcagatgcc tgtgaacaac ttctgctagt ttagcactaa
     3961 tacccgggac acgggcaagt tgatcagaag tcgcctccat aatatttttc acagacccga
     4021 atgtttcgag aagtgcccgg gcgcgtgcgg tgccgacgcc ggggatgtca cttaaggcag
     4081 atttcaggac agcaccgctt cgttttttgc gatgataggt aatcgcgaag cggtgcgcct
     4141 cattgcgcag accggcaagc atccggacaa cctggcttcc ctgaggcggc accagcggat
     4201 tgctcctgcc cggcaggaaa aaacgttcgg gcagaacggc gcctttttcg gtgcgccctt
     4261 cagcgatgct gacacagggc agtgaagaaa gccccaattc tctcaaaaca gacaaggcaa
     4321 tattcagatg ccctttcccg ccgtcaatca aaacaagatc cggaaggtcc gcttcccgga
     4381 tggcgcggct gaatcgccgt gtcagtactt cacgcatggc attgaaatca tcctgcgtga
     4441 cctggctctt aatctgatag cgccggtacc gggacttgtc cggctcgcca tgggtcagga
     4501 cggacatggc ggcaacagtt ttgtcgccct gatgggttga aatgtcaaag cattcaattc
     4561 ttgtcggagc agcgggcagt ttgaacagcg ctgcaatctg ctccagcccg tcctgcagtg
     4621 ctttttctcg gccgcgacgg ctgataaaag acgcttccgc attgtgctcc gccagtttga
     4681 ccagttcttt caggcgcccc cgcttcggca ccgttaatgt gacagcccgc tcccttttct
     4741 cggaaagaat ttccgtgagc gtttcctgat cttccagttc tacgggaagt acaactgttt
     4801 ttggcaccac tggagtcgct tcataaaatt gcagaataaa agagctgaac aattcgttca
     4861 gcggcacttc cacatggtca aaagaaaatg tgtcgccgcc ggtcatggca ttgttccggt
     4921 aatacagcac ctgaaattca acaaagcgcc cttccgcata catgccgaag acatcctgtt
     4981 cttccgtgcc ctccagtgcg acagtgtgct gcggctgcag agtggtttgc agatcaacca
     5041 ggcgatcgcg cagtacggca gcttcttcaa aacgcagttg ctccgccagt gccgttattt
     5101 cttcgcgcag ctgtttttcc aggctccggc ttttaccttc cagaatcata agtacctgtt
     5161 ccacgagccc cgcatattct tcttcgttaa taaaaccgac acacggggct ttgcactgct
     5221 tgatctggta ataaatgcag ggccggctgc ggttgttcag aacactgtcg gagcaggtac
     5281 gcagcggcac aagtttctgc agctgtttca gggtttttct tgctgccttc gtatcgtgat
     5341 agggaccgaa atagcgggcg ccgtctttct tcctccggcg caccacggtc aggcgcggga
     5401 aacgttctcc cggatggatg cgaaggctga tgaaagtttt atcgtcccgg agccgcacat
     5461 tatagcgggg cttgtgcagc ttgatcaggg tattctcaag aagcagcgct tctttttcac
     5521 tggagacggt cagatattcc actgaagcca cgcgacgcat cagaaacttg acgctgtacc
     5581 ggctgtctgt cagctgaatg tagctgcgaa tccggtttcg gaggttgatg gctttcccca
     5641 cgtaaagcac caccccgtct tcatcgcgca taagatagca gccgggctct gtaggcgcat
     5701 ctgctgcact gatcgccggt ggcttttctt ttttttctgg tgcgttacgg ttctctggtt
     5761 tatctttcat actggttctt atagtcccgg tctgttattt taccagagac agggcagagc
     5821 aggggacttt tcaaaggaag caccgatcgc tttaaacata aaaagaaaag agcagataaa
     5881 ccggtgtgat ctatctgctc tgaatcggcg gcagcggtgc tattgttttc atgcatgaaa
     5941 aagagtcatt tcagatccct gcagagcagc tttctttacc gaagcctttc cttaaacgtg
     6001 ttataaaccg ccacaggagc cagcaggagg caagaacggt aagtaccagc agaagcacgg
     6061 tctgaatatt aaaggcgaag gcatttttta gatagtacat gcctgtatct tgtttcctga
     6121 gtttgttgtt cagccagcgc agtgctttat cgacaaagga tgtttttgtt atttctatgg
     6181 tactcaggaa aagctggcct cgtgtccgaa ggtcttcgtc ttcaagatga aacgccggct
     6241 tgccttcttt ccaggaagca gtgtgaaaga gcatgtcgga gcgattcgta atgccgatgc
     6301 aactctcctg cggaacctgg agcacaaatc ccttcaaagc ggcaaagtcc ggattcagga
     6361 tgacatccga caccgtgatc cgttccttcc agccatcacg cattgtaccg cctgtttccg
     6421 aataggtcag cacttccact tctgcaggga tataataagc gccccgggct tcttttttca
     6481 gtacatggat ttcctgcacg tagtgagagg gaatcgttcc ctgatagtct ttggcaaaga
     6541 ctttatcacc gcgccagccg aggtctttgc cgtattcttt gctgaagaca gccttttgca
     6601 ggctgtaatt ttctgtcggc gacagccaca gctttatctg tccatattca gtatcgcttt
     6661 ccacgatgca gctcgccgca ccgccgagtt ccccgtcact tacctgaaag gcataggact
     6721 ccagcagttc aggtattcgc agatgactta aggaaagact gtgacctgtt actacggcgg
     6781 tacatccgaa agaagcatcc aaggacgccc tgccgtcgag tttcggatcc agatcaagat
     6841 agcggccgcc ttcctcatcg tagccgtatt ccaccgccca atttcccgct tccttaaaca
     6901 ggccggattc ccgggagaat tggcgaaggg aaccgctttc ggatcttgct actttacccg
     6961 tagccacgtc gatggttcgg ctatcccagg aggcgtgcca cccctttgca tcatcaatga
     7021 aaaaattgac atcctcatag ccacggagtt tttgcctggt ttctgtgtcg ttttttcctt
     7081 ccggaacaat cttttcgcct acggaactga tgtgcatggc tgcgctgccc agccatccgt
     7141 aggagtcacg aaaagcagcc aggatcgctt cctcatccag agccctgact gccggggcga
     7201 taagaaaaca ctgtacagcc aacgcagcaa cagtatattt tcgttgtctc ataaaggttt
     7261 tcatgactta tctccttttt attgttttgc gtagatgttg attgatctat atcgtggtaa
     7321 ttataacata gccgtggcag tttggcaaga ggttgaaagg aagaagattc acgtcagatg
     7381 cagtgctcat agcggaagcg tttctttact ttcatttttc ctgccgggtt gcattatata
     7441 aggtatgctt ccggtctgcg ctccattgct ttttttgccg ttgctgtctg ccgggtgtgt
     7501 tgtgctaatt tgctatttta atctggcttt tgctacaatg tgtctctgtg cgtagtcgtg
     7561 tctgtaagaa acggctgtcg ctccctgcat ttggagtgta ctccgcagca ccttccctca
     7621 gatttatctg aacggctggt ctgtgttttt caggaggaca gagggtctca agtcttttcc
     7681 ggcagatcca taggtattcg taaatatgtt tgagaatctg gcaaaacgtc ttgactccgt
     7741 attcaaaaca atacgtggcc aggggcggtt gacagaagcc aatatgcgcg atgcgatgca
     7801 gcagatccgc accgcattgc tcgaagctga tgtcaattac caggtagtca aaaagtttat
     7861 cgcagaagtg caggaagcgg cgctgggcca ggaagtgctg gcgagcattc acccgggtca
     7921 gcagatcgtg aagatcgttc atgacaccct cgtgcggacc atgggcgaga agaacgagcc
     7981 gctgaaccgt gcttccaaag ggccgactgt tatcatgctc tgcggtttgc aggggcaggg
     8041 taaaacaacc agcgccggga aactggcggt gcatctgaaa aaatcagggc ataagaccct
     8101 gcttgtcggc gcggacgtat atcgtcccgc cgcaataaaa cagcttgaaa ttgtggcgaa
     8161 gcaggctcag gcggaaatgt tcagtctggg cgagcatgcc gatccggtgg aaacctgtct
     8221 catggcgcgg ggcgaagcga gtcttcgcgg ttgcgacacg attattctgg gtactgccgg
     8281 acgccttcac gttgacgaag agatgatgct tgaagcacgt caaatcgctg aacag
//
LOCUS       DAOPWB010000131         8278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_8631_length_8278_cov_5.121002, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000131 DAOPWB010000000
VERSION     DAOPWB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8631_length_8278_cov_5.121002"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKN92_10720"
     CDS             <1..652
                     /locus_tag="PKN92_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49308.1"
                     /translation="RLIYKSVSASLLILACLPLLWSCNSKSVSDEYPAPGEMRVVSVT
                     RLERPKQTLNLIRNGSFKDWMPGSPICSGFSAPNPEFSKVFRGETGNGANLVMVQQWS
                     RAETNSDPSTCLHTVVDALKADTEYCLSVIAESAGNVSAVLMAVERDSQGGLVMLNPD
                     ILSTLPGTQLIKQYTGTFRTQSGGTVLIGAAARSAGGKDAGIKWHEWLLFEASEKK"
     gene            659..1447
                     /locus_tag="PKN92_10725"
     CDS             659..1447
                     /locus_tag="PKN92_10725"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNZ49309.1"
                     /translation="MERKDKRLLLFLLSITCLAGYAFAEHQPGETQDFGSIPFVWCPP
                     GQFVMGTGSAASAEGDPSWFSDEQPAREVTIEKGFWISRSEITREQWALYMQTRPWAG
                     VDSEENSALAATGMDVRACREFAEKLGDSIKSKCTLPSEEEWEYACRAGGTDPEYGCA
                     SLETLGEYAWHRWNSEGGKLHPVGQKKPNAWGISDMHGNAWEWTATPYLPLHNTGESG
                     LSGYSVIRGGSAAATPVFLRCASRLGRADSAGSPLLGFRVIIVE"
     gene            1550..3841
                     /locus_tag="PKN92_10730"
     CDS             1550..3841
                     /locus_tag="PKN92_10730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ49310.1"
                     /translation="MKKKLIRLLHLHDLFCSPTQWTYRQMLKERDKAFPRSFEQVHCD
                     GIPGLVSIILPVHNGARYLSEAIESVRAQDYPHWELIIVDDGSTDGSAAIALDYTRQD
                     HRIRYIYQENQKLPGALNTGHAEAKGEFITWTSDDNRLKTNFLSVLVQEMKDRPSVDM
                     LYANLEVIDDAGRITGTHNYYRDYQYPEGSGQIQLPHHVSMLHAWNFIGAAFLYRRRA
                     IILLGGYSAYWCICEDYDFFLRVNAQMTVRHTRFLEALYQYRVHLASLTSRSGDSFIA
                     KKLEALQIFDDGRRDLLSGPAAWFIQADPSAACCHIRDQLKQLVAAQGHFLPEGDISA
                     IRWGTPVIFVSVTENGASAPPPEAFFCHARIRLAAGPTPPSPADSGDWTLRLSTEAPE
                     VAPGNPFHSGVLSASEPEVLFHLMHAHGAARFLQTYETFAAAPEDNPPDISVLICTNR
                     RIEFCERALRSLIRQSLAPSRYEIIVVNNAPGKHDYQAAINSFSPPPGLKVQEIPALP
                     GGLSYARNVGAAAARGEILLYMDDDAEADYCLLEETVNAYKSHPAAGGGGGSILLSPP
                     DPAPWWWGPELAPFWSEFRPDEKECRTVTDWLQFPYGANLSIRRKVLWAIGGFRSRYG
                     PGGRKTSWGDDTVVCLQIRQAGHDILLTPKARVFHHVDPARFSLSELFRMCRQSYSTM
                     SLMIQEKRIPANMNFITAGLRAAVRFALALVPVPHPLSWRIRQLALAQGLIACSVALL
                     RYALYPLFTRPPESANPPSERIR"
     gene            3853..4995
                     /gene="wecB"
                     /locus_tag="PKN92_10735"
     CDS             3853..4995
                     /gene="wecB"
                     /locus_tag="PKN92_10735"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNZ49311.1"
                     /translation="MKIAIVFGTRPEAIKLAPLYLQLKKEPACFEPLLWVTGQHRQML
                     DQVMATFHLVPDRDFNLMKPGQSLTDVTCAVLTHLQDAFAEQRPDLLIVQGDTTTVFA
                     GALAAFYARIPVGHVEAGLRTWNRHSPWPEEINRQLSSSLAELHFAPTAKARENLLRT
                     NIDERAIWVTGNTVIDALDIVVKQVSEKPPAFADDFPLDLLADGRRMVLVTGHRRESF
                     GSGFENICQALLTLSRKYSDTEFIYPVHMNPNVRKPVNDILRGQHNIHLIEPLAYENF
                     VWAMNRAYFLLSDSGGVQEEAPHLGKPVIVMRGTTERPEAIEAGTSLLVGTDREKIIN
                     VCSTLLDDDNAYKAMSLAANPFGDGQASVRIAEAIKEHFRLFPERP"
     gene            5013..5966
                     /locus_tag="PKN92_10740"
     CDS             5013..5966
                     /locus_tag="PKN92_10740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ49312.1"
                     /translation="MTDSFVHPVISIITPARNQERYIQEMLRSIQAQSFQQWECIVVD
                     DASTDGTADAVHSLHDPRIRLISHEVRQGVSHARNTGMAHAAGTFLYFLDGDDLLFPG
                     SLERFIRCFEAHPRYSVVYGEVQILASDGSPAGKAKAPLFHPRPTGNILTALLKRNLI
                     PTGALCVRAETAQSAGGFNPSLSIAEDWEYWCRLASLTPFFYLGAKPLAGYRIHSAGT
                     SRSGKYGADAGLSAVDAIFSNPSIRQQAGAKKSASLKKEAQASTYEYAANHCLRNRQW
                     QEARQLLRKTLGHSFFRPRAWILLFFACIGFLPGFVERHLK"
     gene            complement(5980..7455)
                     /gene="rfaE2"
                     /locus_tag="PKN92_10745"
     CDS             complement(5980..7455)
                     /gene="rfaE2"
                     /locus_tag="PKN92_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNZ49313.1"
                     /translation="MDTKRCLELLNQFEKVRLLAIGDIYLDEYVYGVVTEISLEAPIP
                     VVEVQERRYNPGASGNAACNAASLGAQVTMLGVIGTDTNGGIIRREFQKRNVNADHLT
                     EDPGRPTNTYGKWRAGGHNIPTQEMLRTDTPRPKMIEGAVEQQLIDKIKLLAAQVDAI
                     MIGDQVSATISDRVLETIVSCAKEHNLITTADSRARAGMFKGITLITPNDMEAQLASG
                     IQITDDASLEKAGKLLLERSDMVMITLGPDGIACFTREKGMEKVAGRPVRAVDVTGAG
                     DTVIAAATLALASGATLAEAAFLGNLAAGIAVTQTGVVTVSNAEVRDALVEGVSTKTE
                     KQKNREQLQAIVKRLKREGKKVVWTNGCFDILHVGHIMYLQEARNQGDIMVVGLNSDA
                     SVQAIKGPERPVVCEEDRAQVLAALECVDYIVIFDEKTPLPLIALLEPDVYAKGGDYT
                     VDTIDQEERQAVESYGGKIAIIPGREGKSTTWIINKITGAK"
     gene            7627..7857
                     /locus_tag="PKN92_10750"
     CDS             7627..7857
                     /locus_tag="PKN92_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNZ49314.1"
                     /translation="MPLFLYTCKKCGTSSEILVRSDKEKPECPECGSRQMEKQLSRFA
                     AVSGSSTPELPCAGGACSLPYGGGCPGGSCPL"
     gene            7889..8263
                     /locus_tag="PKN92_10755"
     CDS             7889..8263
                     /locus_tag="PKN92_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49315.1"
                     /translation="MPKKQPDTSPVSLKVLFLKGFKEAHRRRPLSFYLLLLIPLVLLL
                     GAHIFRRPVSLLHFAGIVLLLFLFFWIIMIRAVRDLFSLYKQMRAEQLRVYQETLGSA
                     EFKASSNPADAAEVPSQQKSEE"
BASE COUNT         1859 a         2284 c         2119 g         2016 t
ORIGIN      
        1 ccggttgata tacaagagcg tttcggcttc ccttctgatt ctcgcctgcc ttccgctttt
       61 atggagctgc aattccaaat ctgtatccga tgagtatcct gcccccggcg aaatgcgtgt
      121 tgtttctgta acacgactgg aacgcccgaa acagacgctc aacctgatcc ggaacggttc
      181 gttcaaagac tggatgccgg gaagccctat ctgttccggc ttttcagccc cgaatcccga
      241 gttttccaaa gtatttcggg gggaaaccgg caacggtgcg aatctggtta tggttcagca
      301 atggagtcgg gcggaaacaa attccgatcc ctctacctgc ctccacacgg tcgttgacgc
      361 cctgaaagcg gatacagaat actgcctttc cgtgattgcg gaaagtgcgg gaaatgtttc
      421 tgccgtgctg atggctgtcg agcgagacag tcagggcggg ctggttatgc tgaatccgga
      481 catactcagc acacttcccg gcactcagct gatcaaacaa tatacgggca cattccggac
      541 gcagtcgggc ggtactgttc tgatcggggc agcagcccgc tctgcaggcg gtaaagatgc
      601 cggaatcaaa tggcacgagt ggcttctttt tgaagcatcc gaaaaaaagt aagcgaccat
      661 ggagagaaaa gacaaaagac tgctgctttt cctgctcagt atcacatgcc ttgcgggata
      721 tgcatttgca gagcaccagc ccggtgaaac gcaggacttc ggctccattc cttttgtatg
      781 gtgcccgcca ggccagtttg tcatgggcac tgggagcgct gcttccgccg aaggcgaccc
      841 ttcctggttc agcgatgaac agcctgcgcg tgaagtcacc atagaaaaag gcttctggat
      901 cagccgctcg gagattaccc gggaacagtg ggcgctttat atgcagacgc gcccctgggc
      961 aggcgtggac tcggaggaga actccgccct tgctgcaacc gggatggatg tgcgggcatg
     1021 tagggaattt gcggaaaaac tgggagactc cattaaaagc aaatgcacgc ttccctccga
     1081 agaagaatgg gaatatgcat gccgggcagg aggaactgat ccggagtacg gctgcgcctc
     1141 tctggaaaca ttgggggaat atgcctggca tcgctggaac agcgaaggcg ggaaactgca
     1201 tcccgtcggc cagaaaaaac ccaatgcctg gggcatctct gatatgcacg gcaacgcctg
     1261 ggaatggaca gcaacacctt atctcccgct gcacaataca ggcgaatccg ggctaagcgg
     1321 gtattcggtt atccggggag gaagtgcggc ggcaactccg gtatttctac gttgtgcctc
     1381 acgtctgggc cgggcggaca gtgccggttc cccccttctt ggctttcggg tgattattgt
     1441 tgaataatgc tcatagagtc cgctgctctc cacggacgca cctccgattt ttccgctccg
     1501 gcaatgaagg ttaatttgtg tcgatcaccc tgaagaagag aaccggaaga tgaaaaaaaa
     1561 gctgatcagg ctgctgcatc tacacgatct tttctgctct cctacgcaat ggacgtatcg
     1621 gcagatgctg aaagaacggg acaaagcctt cccccggagt tttgagcagg ttcattgcga
     1681 tgggattccg ggcctggttt ccatcatcct gcctgtccat aatggcgcac gttatctgtc
     1741 ggaagccatt gaatccgtgc gggctcagga ctatccgcac tgggaactta tcattgtaga
     1801 cgacggctct accgatggca gcgcagcgat tgctctggac tatacccgac aggatcaccg
     1861 tatccggtat atctatcagg aaaaccaaaa actccccggc gcgctcaaca caggccacgc
     1921 agaagcgaag ggagaattca tcacctggac aagcgatgac aacagactga agaccaattt
     1981 tctttctgtc cttgtacagg agatgaagga tcgtccgtct gtggatatgc tgtatgccaa
     2041 tctggaagtc atcgacgatg cagggcgcat taccggaacg cacaactatt accgtgatta
     2101 ccagtatccg gaaggaagcg gacaaataca gcttccgcac catgtctcca tgctgcatgc
     2161 gtggaatttc ataggcgccg cattcttgta ccgacgccgc gcaattatcc tgctgggcgg
     2221 ctacagcgcc tattggtgta tttgtgaaga ttatgatttt tttcttcgtg tcaatgcaca
     2281 gatgactgtt cgacatacac gctttttaga agcgctttat caatatcggg tgcacctggc
     2341 gtcactcacc tcccggtcag gggattcatt tattgcaaag aaactggaag cactgcagat
     2401 ttttgatgac ggacgccggg atcttttatc cgggccggca gcatggttta ttcaggctga
     2461 cccttcagcc gcctgctgcc acattcggga tcagttaaaa caactggtgg cagctcaagg
     2521 tcattttctt ccggaaggag acatctccgc catccgttgg ggcacaccgg ttatttttgt
     2581 ttcggtcact gaaaacgggg cttctgcacc tcccccggaa gcatttttct gtcatgcccg
     2641 catacggctg gcagcaggcc ccacgcctcc ttcccccgca gattccggcg actggacgct
     2701 ccgccttagc acagaagctc ccgaagttgc ccccggcaac ccttttcact ccggcgttct
     2761 ctctgcttca gaaccggaag tcctgtttca tcttatgcac gcccacggcg cagcacgctt
     2821 cctgcagact tatgaaactt ttgccgccgc acccgaagat aatccgccgg acatttctgt
     2881 tctcatctgt acaaaccgac ggattgaatt ttgtgaaaga gcgctgcgct cactgatccg
     2941 gcaaagtctg gctccgtccc gctatgagat tattgtcgtc aacaatgctc ccggaaagca
     3001 cgattatcag gcggctatta actctttttc cccgcctccg ggattgaaag tgcaggaaat
     3061 ccccgctttg ccaggcggtc tgtcctatgc gaggaatgtg ggtgcagctg cggcgcgggg
     3121 agaaatactg ctgtatatgg acgatgacgc ggaagcggat tattgcctgc tggaagaaac
     3181 ggttaacgcc tataaaagcc acccggctgc agggggcggc ggcggaagta ttcttctttc
     3241 tcctccggat ccggccccgt ggtggtgggg tccggaactc gcccctttct ggtctgaatt
     3301 tcgccctgat gaaaaagagt gccgcactgt tacagactgg ctgcaattcc cctacggcgc
     3361 gaatttaagt ataaggcgga aagttctgtg ggcgatcggc ggtttcagaa gcaggtacgg
     3421 tccgggcggg agaaagacat cctggggtga tgacacggtc gtctgcctgc aaatcagaca
     3481 ggccggtcat gatattcttc tcaccccgaa agcccgggtt tttcatcacg tcgatcctgc
     3541 acgtttctcg ctgtcggaac tttttcgcat gtgccgccag tcttacagta ccatgtcact
     3601 tatgattcag gaaaaacgga tcccggcaaa catgaatttt atcactgccg ggctgcgtgc
     3661 ggcggtgcgt tttgcactgg cgctggtgcc ggtgccgcat cctctatcgt ggagaatccg
     3721 gcagctggca ctggcgcaag gactgatcgc ctgctccgtt gcgctgctgc ggtacgcgct
     3781 ttatccgctt tttacgcgtc cgcctgaatc tgcaaatccc ccatctgaaa ggatcaggta
     3841 gtgcaacttc ccatgaaaat agccattgtc ttcggaacaa gacctgaagc aataaaactg
     3901 gcacccctct atctgcaact gaagaaggaa ccggcttgtt ttgagccttt attgtgggtg
     3961 accgggcagc acaggcagat gctggatcag gtaatggcaa cctttcatct ggtcccggat
     4021 agggacttta atctgatgaa accgggacaa agccttactg atgtaacctg tgccgtcctg
     4081 acgcatctgc aggatgcctt cgccgaacaa cgtcctgatc tgctgattgt tcagggagat
     4141 acaacgacag tcttcgccgg agcccttgcc gctttttacg cccggattcc tgtcggacat
     4201 gtggaagccg gactccgcac ctggaacaga cattcgccct ggccggagga aatcaaccgg
     4261 cagctcagtt cttcgctggc agaactgcat tttgcaccca cagccaaggc gcgcgaaaat
     4321 cttttgcgca caaacataga tgaacgtgcc atctgggtta cgggcaacac ggttatcgat
     4381 gcgctggaca ttgttgtcaa acaggtcagc gaaaagcctc ctgctttcgc cgatgatttt
     4441 cctttggatc ttcttgccga tggcagacgt atggttcttg tgacaggaca tcggcgcgaa
     4501 agtttcggca gcggctttga aaacatctgc caggcgcttc tgacactgtc ccggaaatac
     4561 agcgatacgg aatttattta tcccgtgcat atgaatccca atgtgcgcaa acccgtcaat
     4621 gatatcctca ggggacagca taacatccac ctgattgagc cgctcgccta tgagaacttt
     4681 gtctgggcaa tgaatcgcgc ttattttctg ctgagcgatt cgggaggcgt tcaggaggaa
     4741 gcgccgcatc ttggcaaacc tgtgattgtt atgcgtggca ccacggagcg ccccgaagcg
     4801 attgaagcag gcacttcact gcttgtgggc acagaccggg aaaagattat caatgtctgc
     4861 tcgactctgc tggacgacga taacgcatac aaagccatga gcctcgccgc caatcctttc
     4921 ggtgacggtc aggcgtcggt gcgcatagca gaggcgatca aggaacactt tcgtcttttt
     4981 cccgaaagac cctagaaagc ggctgccttt ttatgactga ttcctttgtt catcctgtca
     5041 tttccatcat cactcctgcc cgaaatcagg agaggtatat acaggagatg ctgcgcagca
     5101 ttcaggctca gtcctttcag caatgggaat gtattgtggt ggatgatgct tccacagacg
     5161 gcacagcgga tgcagtccat tctctgcatg atccaagaat tcgtctgatt tcccatgaag
     5221 tacgccaagg cgtttctcat gcgagaaaca caggcatggc gcacgccgcc ggtacatttc
     5281 tgtattttct cgatggtgac gacctgctgt ttcccgggag ccttgaacgt tttattcgct
     5341 gctttgaggc ccatccacgg tatagtgttg tttatggaga agtgcagata cttgcgtcag
     5401 acggaagccc cgccggaaaa gcaaaagccc cgttatttca tccgcgtccc acaggcaata
     5461 tcctgactgc cttattaaaa cgtaacctta ttcccaccgg tgcactctgt gtacgggcag
     5521 aaacggcaca gtccgccgga gggtttaacc cgtctctttc cattgcggaa gactgggaat
     5581 actggtgccg tctcgcttca cttacgccct ttttctatct gggcgccaag ccccttgccg
     5641 ggtacagaat ccattctgcc ggtacctccc gatcaggaaa gtacggagcg gacgcagggc
     5701 tgtctgctgt tgatgccatt ttttccaacc cttccatacg ccagcaggca ggcgcaaaaa
     5761 aaagcgcctc tttaaaaaaa gaggcgcagg catcaaccta tgaatacgct gcaaaccact
     5821 gccttcgaaa ccggcagtgg caggaggcac ggcaactgct ccgcaaaaca ctggggcatt
     5881 ctttttttcg gccccgcgcc tggatactgc tcttttttgc ctgtatcgga ttcctgccag
     5941 gttttgtgga gcggcatttg aagtaatgcc ctgccgacgt tattttgcgc cggtaatttt
     6001 attgatgatc caggtggtcg acttgccttc acggccgggg ataatcgcta tttttccgcc
     6061 ataggactcc acagcctgac gttcttcctg atcgatggta tccacagtat aatcaccgcc
     6121 ttttgcatac acatcaggct ccagcagtgc aattaaaggc agcggcgttt tttcatcaaa
     6181 gataacaata taatccacac attcaagcgc tgccagaacc tgagcacgat cttcttcaca
     6241 gaccacagga cgctccggcc ctttgatcgc ctgaacggaa gcatcgctat tcagccccac
     6301 caccataatg tccccctgat tacgtgcttc ctgcagatac atgatatgcc ccacatgaag
     6361 gatatcaaag cagccgttcg tccatacgac ttttttccct tcccgtttca gccgtttcac
     6421 aattgcctgc agctgctcgc gatttttctg cttctctgtc ttcgttgaaa ccccttcgac
     6481 gagtgcgtca cgcacttctg cattggaaac cgtcaccaca ccggtttgcg tcacggctat
     6541 acctgccgcc agattgccca ggaaggctgc ttcggctaag gtggctccgc ttgccaatgc
     6601 caaagtagcc gcggcaatca ctgtgtctcc ggctccggtc acatctacag cacgaacggg
     6661 gcgccctgcc accttttcca ttcccttctc ccgcgtaaaa caggcgatac catcgggacc
     6721 gagagtaatc atgaccatat cggatcgttc aagcaggagt tttccggctt tttcaagtga
     6781 cgcatcatcg gtaatttgaa tgcccgaggc cagctgcgct tccatatcat tgggggtgat
     6841 cagcgtaatc cctttaaaca tgcccgcccg ggcacgggaa tcggcggtcg tgatcagatt
     6901 atgctccttt gcgcaggaca cgattgtttc cagcacacgg tcactgatgg tggcggatac
     6961 ctggtcacca atcataatag cgtccacctg cgccgccagg agtttaattt tatcaataag
     7021 ctgctgctct acagcccctt caatcatttt gggccggggc gtatcggtac gcagcatttc
     7081 ctgcgtggga atattgtgtc cgccggcgcg ccattttccg tacgtattgg tcggccgtcc
     7141 cggatcttcc gtcagatgat ccgcattgac attgcgcttc tggaactcac gccggataat
     7201 gccgccattg gtgtccgtac cgattacgcc cagcatggtc acctgtgcgc ccagcgacgc
     7261 cgcattgcag gcggcatttc ccgatgcacc gggattgtac ctgcgctcct gaacctcgac
     7321 cacagggata ggcgcttcaa gactgatttc cgttacaaca ccatacacat actcatcaag
     7381 ataaatatcg ccaatcgcga gaagccgaac tttttcgaac tgattgagca attcaagaca
     7441 gcgctttgta tccatgaaat cttcctgcct taatgttaac ggaacgcaat aaaacaaagg
     7501 gtagtttatc ataactgccg ggctctgccg gaatttaatt ttttactgat gcgttagaga
     7561 agcgaagcat gttatagtat tttcggtggt atgagtgtaa tttctatccg gtaaaggagc
     7621 ctgacaatgc ctctttttct atatacctgc aaaaaatgcg gcacttcgtc tgagattctg
     7681 gttcgctcag acaaagaaaa acctgaatgt cctgaatgcg gatccaggca gatggaaaag
     7741 caactgagtc gctttgctgc tgtcagcgga agcagcacac cggaacttcc ctgtgccggg
     7801 ggtgcctgca gtctccctta tggcggcggc tgtccgggcg gctcctgccc gctttaaggc
     7861 ataaccaaca cggcggcgga ttttctttat gccaaagaaa caacccgata catcacctgt
     7921 atctttgaag gttcttttcc ttaaaggctt taaagaagcg caccgccgca ggcctctcag
     7981 cttttatctt ctcctgctta tcccgctcgt gctcctgctg ggcgcgcata tcttccggcg
     8041 ccctgtatcg cttttgcatt ttgccgggat cgtgcttctt ctctttctgt ttttctggat
     8101 tatcatgatc cgggctgtcc gggatctgtt ttccctttac aaacagatgc gtgctgagca
     8161 gctgcgcgtc tatcaggaaa cgctcggcag cgcagaattc aaagcctctt caaatccggc
     8221 agatgcggca gaagtccctt cacaacagaa gtctgaagaa taaaaaacgc tgcggcat
//
LOCUS       DAOPWB010000132         8190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_8748_length_8190_cov_5.332391, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000132 DAOPWB010000000
VERSION     DAOPWB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8748_length_8190_cov_5.332391"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PKN92_10760"
     CDS             <1..645
                     /locus_tag="PKN92_10760"
                     /inference="COORDINATES: protein motif:HMM:NF037222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49316.1"
                     /translation="VHALLDPHEQAVYVVNSLYEAQDGLSLDVIVLDPAGSVLSQESF
                     TVSVAADGCTRAGALRIPESAGNLRFVKMLLKNKDGVILSENVYWLSATPDVPGASGH
                     KDSVFYTHPKSVADYSGLNTLPETSVESRITSDGTNHTITLENKSDRIAFQMELALLP
                     ADSDLSLAPVYWSDNYVTLFPGESRTLQAQTSSGSTAPLRLRVQGFNVPETISD"
     gene            complement(661..1890)
                     /locus_tag="PKN92_10765"
     CDS             complement(661..1890)
                     /locus_tag="PKN92_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ49317.1"
                     /translation="MNSRERVLAALNFEAFDRTPRDLGGMPSTGISCFAYPALVQALG
                     LPPRRPRVYDTGQMLALPDTDVLDALGCDVVTLQPDVTNAFPQPELWHPYHFNNRLDA
                     EVLDLSPFSALPDGSVRQTPPGSVMPPHAYVFDVEHAGHALVLSGDIPRPDLDEIRAS
                     LKKRRWSRERKKQLLETAKRVRDTSDRAVLFNGLQPGMGITNFTGLALFPMLCLTEPE
                     FVEELHGMLLAFTLEALEDWLPELGHCIDIYMCSADDWGTQSALIASPQIYRTLFKPF
                     YKKMNDAIHQLAPGVKTFLHSCGAIYDLLDDIAESGFDILNPVQWTAGGHSVQEWKDK
                     ARHRIALWGGGVNTQHTLPRGTVQEVEEEVRRTVSCLQEDSGYVFCAIHNILAEIDPE
                     KIIAMYRAAGDMAPTRQ"
     gene            2082..2858
                     /locus_tag="PKN92_10770"
     CDS             2082..2858
                     /locus_tag="PKN92_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49318.1"
                     /translation="MKNAHPLSALFLFPALCLMLMLLSGCGAEILTVTAVQGELAAQN
                     AKSATKQLAYVKDKMGSVSFDQAVQAYRAEHGSNPPTLDALVPGYLPQIPVQPDGSPY
                     YYDPATGTLSASAPAGASSKQDQEMMLAIRNAITRYGTATGYYPPTLDALYPNYMTRL
                     PRTSAGQEFVYNNQNGQISLPGQVSPTAPARTAGNRSAGIGGTGPMGEVMTGIGIQQE
                     LGRMNTSGASAAGSRTRSSAQDFSNQHTNTQNRVMDQLGL"
     gene            3113..4342
                     /locus_tag="PKN92_10775"
     CDS             3113..4342
                     /locus_tag="PKN92_10775"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ49319.1"
                     /translation="MTRVFHSFLILGVFALLSPAFAADWVSLTPDNSLDGWRVLAGEW
                     SVSEDGVISGRAAKDQNCWLLYDKEEFSDFELSLEFRTPVPTNGGVQLRSHWLPRMPL
                     AEGEEVNAAPRQMYGYQANIETRKRLGTGTLMEENGRGYLAEPSADAAKTLKQRDWNT
                     MRILAQGPVVEVYLHDVLAARIEDEAFISGFIALQLFPYEAGEDFTEIEYRNVRIKNL
                     RDDSNWRSLFDGTTLTGWKEWGSEKWSVEDGVIWGKSGPKRSEGYLSTDEIFKDFRVR
                     GSFNMTGDGNYGLFYHSSIKLREDDGYPLIAGLQGEVDPGYPGPSGWVYESYKRGWLV
                     KPDPTSMQSVAVRPGKWNTIEIRSEGNHVTTWINGIRVLDLVDEGQCLFEGFFALQLH
                     TGGADGIGWKDLFVLNR"
     gene            complement(4415..4858)
                     /gene="rpiB"
                     /locus_tag="PKN92_10780"
     CDS             complement(4415..4858)
                     /gene="rpiB"
                     /locus_tag="PKN92_10780"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNZ49320.1"
                     /translation="MIIAVGSDHAGYEAPEPLYKPSVIARLEAMGHTVIDCGTNGPES
                     VDYPDFANAVCKEVLEGRARYGVLICGTGIGIGMAANRHPGIRAATCTSREMVHLARA
                     HNDANVIAVGRRILTLDQCLDLITLFLDTAFEGGERHCRRIAKLG"
     gene            complement(4878..5198)
                     /locus_tag="PKN92_10785"
     CDS             complement(4878..5198)
                     /locus_tag="PKN92_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNZ49321.1"
                     /translation="MFPAAEGLPRRILSDSGQVCLRCPEEALARRLCLLRSGALTSTS
                     ANLSGHAPARSAEEAALPGVALVLDGGALPPREPSTVFDAAEGRVVRAGALAAELLYD
                     CWNG"
     gene            complement(5093..5479)
                     /locus_tag="PKN92_10790"
     CDS             complement(5093..5479)
                     /locus_tag="PKN92_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNZ49322.1"
                     /translation="MNIVAPDETGLAQALEALACDEVVAYPTETVYGLAVNPLSEKAL
                     DRLFSVKKREPAFPVLLIVADETQLRPFAAHIDDRTRACMQRFWPGPYLFCFLQRRAF
                     PGAFSATAARFAFVVPRRLSHGGCAC"
     gene            complement(5476..6072)
                     /gene="rdgB"
                     /locus_tag="PKN92_10795"
     CDS             complement(5476..6072)
                     /gene="rdgB"
                     /locus_tag="PKN92_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNZ49323.1"
                     /translation="MKTDLLIGSGNLKKAQELAALLEGLPWRVLSLRDMPDLPEPEET
                     GASFAENAALKAQYYGDAFQIACIADDSGLSVDCLGGAPGVRSARYAGEHATDEENNA
                     KLLEALSDALWHERGARFVCCAAFYTPGGQVHLETGEIAGHIAVAPYGTLGFGYDSLF
                     VPEGEERTFAQMKQEEKQKISHRGIAFRKMRRFLEERS"
     gene            complement(6062..6781)
                     /gene="rph"
                     /locus_tag="PKN92_10800"
     CDS             complement(6062..6781)
                     /gene="rph"
                     /locus_tag="PKN92_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNZ49324.1"
                     /translation="MSLRIDQRTAAMVRPVSITRHFTKHAQGSVLVSFGDTRVLCTAI
                     YAEKPPAWLRGRGKGWVTAEYAMLPSSTQTRISRDNAQRGRAQEISRLIGRSLRAVTD
                     LEAMGECMIQVDCDVLQADGGTRSAAVTGAWVALHDAFSALTAEGRFERIPLFGACAA
                     VSVGIVEGQALLDLCYVEDEAAQADVNIVMDDQLRLIEVQGTAERKPFSREVFQELLT
                     LGEQGITRLLALQQKALNSED"
     gene            complement(6794..>8190)
                     /locus_tag="PKN92_10805"
     CDS             complement(6794..>8190)
                     /locus_tag="PKN92_10805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNZ49325.1"
                     /translation="EDQAVDVYREIEPIGDVLDIVLREYVRELSVEETVEGAISGIMS
                     ALDRNSAYVSAEELEALREETKGEFEGIGVSIKENEDGQIMVFMPILGSPAAEAGVRP
                     YDIITAIDGKSTEGLDTSGAANLIRGRRGSYVNLTILRRTEKEDGTHTEETFDVKVQR
                     ARIPLESIKEFQILHNQVGYIRLNSFSDTTAADLEKHLKELIQQGMRAFVLDLRWNSG
                     GLLSASGEVCELFLPKGSLVTYTRGRKSVNGTSDKDEMRLYTTREAVLPSDMPVVVLT
                     NSETASAAEIVTGALQFYQRAIVVGQRTFGKGSVQTIIPLERPKNSALRLTTALYYTP
                     ADVTIDHQGILPDKEVEMSRDEELALAKQMYSSFENNYENQYKQNHGSMTPGYAATDE
                     TAEDTPLAAAVEILSENLDWPALVEKYHKDIHETQVASDSNTINDPKRALKKSEAPAQ
                     KKSPEEVPPADPVP"
BASE COUNT         1880 a         2349 c         2166 g         1795 t
ORIGIN      
        1 gtgcatgccc ttctggatcc ccatgagcag gcagtgtatg tggtaaacag cctgtatgag
       61 gcgcaggacg ggctttctct cgatgtaatc gttctggatc cggcgggcag cgtactttct
      121 caggaaagct ttaccgtatc cgtcgctgcc gacggctgca cccgcgcggg agcgctccgc
      181 atacctgaat ccgccggaaa cctgcggttt gttaaaatgc ttctgaaaaa taaagacggc
      241 gtcatcctgt cggaaaatgt atactggctt tcggcaacac cggacgtccc cggtgcctcg
      301 ggacacaaag actctgtatt ctatacacac cccaagtctg ttgccgatta cagcgggctg
      361 aatacactcc cggaaacatc cgtcgaaagc cggatcacct ctgatggaac caaccatacc
      421 atcacgctgg aaaataaaag tgaccgtatc gcctttcaga tggaactcgc gctgctgccg
      481 gcggatagtg atctttcact ggcacccgtt tactggagcg ataattatgt gacccttttc
      541 ccgggagaaa gcagaacttt acaggcacaa acgtcttcgg gcagtaccgc gcctctccgc
      601 ctgcgggtac agggcttcaa tgtgcctgag acaatcagcg actgagttcc ggcacgggct
      661 ttattgccgt gtcggtgcca tgtctcccgc cgccctgtac atggcaatga ttttttccgg
      721 atcaatctcc gcaagaatgt tgtgaatggc gcaaaagacg tagccgctgt cttcctgcag
      781 gcaggaaacc gtccgacgca cttcctcctc gacttcctgc acggttccgc gcggaagtgt
      841 atgctgtgta ttcacaccgc cgccccagag ggcaatgcga tgccgggctt tatctttcca
      901 ttcctgaacg ctgtgcccgc ctgcggtcca ttgtaccgga ttaagaatat caaaaccgct
      961 ttccgcaata tcatccagta aatcataaat ggcaccgcat gaatgcagaa aagttttcac
     1021 gccgggcgcc agctgatgaa tggcatcatt catttttttg taaaagggct tgaaaagagt
     1081 cctgtagatt tgaggcgaag caatcagcgc actctgagtg ccccagtcat cggcgctgca
     1141 catgtatatg tcaatgcagt gccccagctc aggcagccag tcttccagtg cctcaagagt
     1201 aaaggcaagc agcatcccgt gcagttcctc aacaaattcg ggctcggtga gacagagcat
     1261 tggaaagagg gcaagccctg taaaattggt gatacccatt ccgggctgaa gcccattgaa
     1321 cagcacagcg cgatccgagg tatcccgcac acgcttcgct gtctccagca gctgtttttt
     1381 ccgctctctg ctccagcgcc tcttcttcag agaagcgcgt atttcatcaa gatccggccg
     1441 gggaatatca ccggacagaa ccaaagcatg accggcgtgc tccacgtcaa aaacatacgc
     1501 atgaggcggc atgactgaac cgggtggagt ctgccggaca ctgccgtcgg gcagtgcgga
     1561 gaaaggcgac aaatccagca cttccgcatc cagccgattg ttgaaatgat agggatgcca
     1621 cagttccggc tgaggaaaag cattggtgac atcgggctgc agtgtaacca catcacatcc
     1681 gagtgcgtcc aatacatccg tatcgggaag agccagcatc tgaccggtgt catagacccg
     1741 cggccttcgg ggaggcagcc cgagcgcctg caccagtgcc gggtaggcga agcagctgat
     1801 cccggtggac ggcataccac cgagatctct gggggtccgg tcaaaggctt caaaattcaa
     1861 agcggcaaga acacgctcgc gactgttcat taaaaaatac tccggctcag gcagaaacga
     1921 ccgcctttat tttttcagcg gcggattcgg aaataaggta taattaagta tacagtaaaa
     1981 taccggatca acaggaacgg gattttcgta cagcagcaga aaagctgtcg agaaatagtg
     2041 tttctttatc cggtgttttc acaggtaaaa ggaacggcgc catgaaaaac gcacaccctc
     2101 tgtccgccct gtttttgttt cccgcccttt gcctgatgct tatgctgctg agcggctgcg
     2161 gtgcggaaat cctgaccgtc acagctgtgc agggcgaact ggcggcgcag aacgccaagt
     2221 cagctacaaa acagcttgct tatgtgaagg acaaaatggg ctccgtcagc tttgatcagg
     2281 cggtacaggc ataccgtgcg gaacatggca gcaacccgcc cacgctggat gcgctggttc
     2341 ccggctatct ccctcagata ccggtacaac ccgacggaag cccctattac tacgatcctg
     2401 cgacaggcac cctttccgcc tcggcaccgg caggagcatc ctccaaacaa gatcaggaga
     2461 tgatgcttgc catccgcaat gccattaccc gttacggaac agcgacgggc tattacccgc
     2521 ctacactgga tgcactctat cctaattata tgaccagact gccccgaacg agcgccggtc
     2581 aggagtttgt gtataacaac cagaacgggc agatctcact gcccggtcag gtgtctccca
     2641 ctgcccctgc cagaacagca ggcaaccgca gcgccgggat agggggcacc gggcccatgg
     2701 gcgaagtgat gacagggatt ggcattcagc aggaactggg gcgtatgaat acaagcggtg
     2761 cctcagccgc cggctcacgc acacgcagca gcgcccagga tttttccaat cagcacacga
     2821 atacccaaaa ccgggttatg gatcaactgg gattataaaa aagctgactc ccctgcactg
     2881 cgacacaact cccctgcggt ggcgcgacac tggtcgtgtg tatggcaccc tccttttccg
     2941 tcagaaaagg aaggatctat tttgcgcccg gatgcaggat tcggtatgat taaatacagg
     3001 agaggcgtgc gtctttcccc ctgttttcaa gaaggtgtct gaccgggtta tgtcagaacg
     3061 ccccctgcat tgccggttaa cctccggcca aaccagaatc gaaggatttg ccatgactcg
     3121 tgtgtttcat tcttttttaa ttctcggcgt ttttgcgctg ctttcccccg cttttgccgc
     3181 tgactgggtc agccttacac cggacaacag tctggacggc tggcgcgttc tcgcaggtga
     3241 atggtctgtg tctgaagacg gtgtaatttc aggtcgtgcc gcaaaagatc agaattgctg
     3301 gctgctttat gacaaagaag agttctccga ctttgagctc tcgctggagt tccgcacacc
     3361 tgttcccaca aacggcggcg tacagctccg ctcccactgg cttccgcgca tgcccctggc
     3421 agaaggggaa gaggtgaacg ctgccccccg ccagatgtat ggctatcagg caaacattga
     3481 gacacgcaag cgtctgggaa cgggaacact tatggaagaa aacggcagag gttacctcgc
     3541 cgaaccttcc gccgatgccg ctaaaacgct caagcagcgt gactggaata ccatgcgtat
     3601 ccttgcgcag ggacccgtgg tggaagtgta tctccatgat gtgctcgccg cccgaattga
     3661 ggacgaggcg tttatcagcg gattcattgc gctgcagctt ttcccctatg aagccggaga
     3721 agactttaca gaaattgaat accgcaatgt gcgcattaaa aatttgcggg acgacagcaa
     3781 ctggcgctcc cttttcgatg gcaccaccct tacaggctgg aaagaatggg gctcggaaaa
     3841 atggagcgtt gaagacggcg ttatctgggg taaaagcgga cccaaacgct ccgaaggata
     3901 tctgtcaacg gatgagattt ttaaagactt ccgggtacgc ggatctttta atatgaccgg
     3961 cgacggcaac tacggacttt tctatcattc ctccatcaag ctgcgggagg acgacgggta
     4021 tcccctgatc gccgggctgc agggcgaagt ggaccccgga tatccggggc cttccggctg
     4081 ggtctatgaa agctacaaac gcggctggct ggtcaaaccg gatccgacaa gcatgcaaag
     4141 cgtggcagtg cgcccgggaa agtggaatac cattgaaatc cgcagcgaag gcaatcatgt
     4201 caccacctgg ataaacggca tccgggtact ggatctcgtg gacgaggggc agtgtctctt
     4261 tgaaggcttc tttgcgctgc agctgcatac aggcggggcg gacggcatcg gctggaaaga
     4321 ccttttcgtg ctgaaccggt aatcccctga aataacagtt tccccggcgg catcctttca
     4381 ttaaaaaagg gtgccgcttt ttttatatcc cgggtcagcc cagtttggct atacgccggc
     4441 agtgccgttc gccgccctca aaagcggtgt caagaaaaag tgtaatcagg tcgagacact
     4501 gatccagcgt cagaatgcgc cgacccaccg cgatcacatt ggcatcatta tgcgcccggg
     4561 caagatgcac catttccctg ctggtacagg tggcggcgcg gatgccgggg tgtctgtttg
     4621 ccgccatgcc gatgccgatg ccggtaccgc agatgagcac accgtaccgg gcacgccctt
     4681 ccagcacttc tttgcataca gcatttgcaa agtcgggata atccaccgat tccggaccat
     4741 tggtgccgca atcaatcaca gtgtgcccca tagcttccag acgggcgatc acggacggtt
     4801 tgtataaggg ttcgggcgcc tcatatccgg catgatccga tccgacagca ataatcataa
     4861 gtacatcctt ttctgtgtca cccgttccaa cagtcataca aaagttcagc ggcgagcgca
     4921 cccgcccgga caacacgtcc ttccgccgca tcaaaaacag tggatggctc ccgaggcgga
     4981 agtgcaccgc cgtccagcac gagggctacc cccggcagtg ctgcctcttc ggcagaccgt
     5041 gccggcgcat gccccgaaag attcgccgat gtggatgtca gcgctccgct ccttagcagg
     5101 cacagccgcc gtgcgagagc ctcctcggga caacgaaggc aaacctggcc gctgtcgctg
     5161 agaatgcgcc ggggaaggcc ctccgctgca ggaaacagaa gagatagggg cctggccaga
     5221 agcgctgcat acaggcgcgg gttctgtcat caatatgcgc ggcaaaaggc cggagctggg
     5281 tctcatctgc cacaatcagg agcacaggaa acgccggctc ccgtttttta acggaaaaaa
     5341 gccgatccag tgccttttcc gacagcggat tcactgccag accgtaaacg gtctcggtgg
     5401 gataggcgac aacctcatcg cacgccagcg cttccagagc ctgtgccagc cctgtctcgt
     5461 cgggtgcgac aatattcatg aacgttcttc cagaaaacgg cgcatttttc ggaaagcaat
     5521 accccgatga cttattttct gcttctcttc ctgtttcatt tgtgcaaagg tgcgttcctc
     5581 tccttcggga acaaaaaggg aatcatagcc gaagccgagc gtgccatagg gagcgacagc
     5641 aatatgtcct gcaatctcgc ccgtttctaa atgtacctga ccgcccggag tataaaaagc
     5701 ggcacagcag acgaaacggg ctccccgttc atgccagagc gcgtcagaca gcgcttccag
     5761 cagtttggcg ttgttctctt catccgtggc atgctccccc gcatagcgcg cagaacgtac
     5821 gccgggagca ccgcctagac aatccacact caaccctgaa tcgtcggcaa tgcaggctat
     5881 ttgaaaggca tcgccgtagt attgcgcctt aagtgcggca ttctcggcga aggaggcgcc
     5941 tgtctcctcg ggctcaggaa gatccggcat atcacgcagg gacagcacgc gccacggcag
     6001 cccctcaagc agtgctgcca gttcctgcgc ttttttcagg tttccgctgc ctataagtaa
     6061 atcagtcttc actattcaaa gccttctgct gcagggcaag aagccgggta atcccctgtt
     6121 cccccaaagt caggagttcc tgaaatactt cccgggaaaa gggttttctt tccgccgttc
     6181 cctgcacttc gatcagcctg agctgatcat ccatgacaat gttcacatct gcctgcgcgg
     6241 cctcgtcttc aacataacaa aggtcaagaa gcgcctgccc ttccacaatg ccgacactga
     6301 cagcagcaca ggcaccgaag agcgggatgc gctcaaacct gccttcggcg gtcaatgcgg
     6361 aaaacgcatc gtggagggca acccaggctc ccgtgacagc ggcactgcgg gtgcctccgt
     6421 ctgcctgcag gacatcgcaa tccacttgaa tcatgcactc gcccatcgcc tccagatcgg
     6481 taacagcacg gaggcttcga cctatcagcc ggctgatttc ctgtgcccgc ccgcgctggg
     6541 cattgtctct ggaaatacga gtctgggtgg acgaggggag catggcatat tcggcggtaa
     6601 cccacccctt gccgcgtccg cgcagccagg caggtggttt ttcggcatag atagcagtac
     6661 agagcacccg ggtatccccg aaggaaacca gtaccgaacc ctgtgcatgt tttgtgaagt
     6721 gccgggtaat ggaaaccggc cgaaccatgg cagctgtacg ctggtcaatt cttagtgaca
     6781 tgcacttttc ctgtcagggc accggatccg caggaggcac ttcttcaggc gatttctttt
     6841 gagccggtgc ttcggatttt ttcagtgcac gtttcggatc attgatggta ttggaatcgg
     6901 aagccacctg tgtctcgtga atatctttgt gatatttttc caccagcgca ggccagtcaa
     6961 gattttcaga cagtatctca accgccgccg ccagaggcgt atcttccgcc gtctcatcag
     7021 tggcggcata tccgggcgtc atgctgccgt gattctgctt gtactgattc tcataattgt
     7081 tctcaaaaga actgtacatc tgctttgcaa gagccagctc ctcatcacga ctcatttcca
     7141 cttctttatc gggaaggatg ccctgatgat caatggtcac atcggcagga gtgtagtaaa
     7201 gagccgtagt caggcgcagc gcagaatttt tcggccgctc gagggggata atagtctgaa
     7261 cacttccctt gccgaaagtc cgctgaccga cgacaatggc acgctggtaa aactgcagtg
     7321 cacctgtcac aatttccgcg gcactggctg tttctgaatt ggttaaaacc acaacaggca
     7381 tatcggaggg aagaaccgct tcccgggttg tgtagagccg catctcatct ttatcagagg
     7441 tgccgttcac cgattttctg ccccgggtgt acgttaccag agagcctttg ggcagaaaga
     7501 gttcgcagac ttctccggat gcggacagca gtccgcctga attccagcgc aaatcaagca
     7561 caaaagcgcg catgccctgc tggatcagct ctttcagatg tttttccaga tccgcagcag
     7621 tcgtatcgct gaatgaattc aggcggatat aacccacttg attatgcagt atctgaaatt
     7681 ctttgatgct ttcgagcgga atccgtgccc gttgtacctt tacatcaaaa gtttcttcgg
     7741 tatgcgtgcc gtcctctttt tccgtacggc gcaggatggt cagattcaca taggagcctc
     7801 tgcgcccgcg gataagattg gcggcgccgg aggtatcgag cccttccgtt gactttccgt
     7861 caatggcagt aatgatgtcg taagggcgta ctcctgcttc cgctgcgggc gagcccagaa
     7921 tgggcataaa taccataatc tgtccgtctt cattttcctt gatggatacg ccgatccctt
     7981 caaattcgcc ttttgtctct tcacgcagcg cttcgagttc ttccgcagag acataggcac
     8041 tgttccgatc aagcgctgac atgatccctg aaatcgcccc ttccacggtc tcttccacac
     8101 tgagttcacg cacatactca cgcagcacga tatcaagcac atcgccgatc ggctcaatct
     8161 cccggtagac atcgacagcc tgatcctctg
//
LOCUS       DAOPWB010000133         8071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_8925_length_8071_cov_4.945359, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000133 DAOPWB010000000
VERSION     DAOPWB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8925_length_8071_cov_4.945359"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKN92_10810"
     CDS             complement(<1..300)
                     /locus_tag="PKN92_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ49326.1"
                     /translation="MNRTLLLIICYGGMISVAICLNLMPVHYTTFSHEFGGLNEEQLG
                     RISALVFAGVVVGVLLSGPLADRFGAALFTFLGLIISSLGILLISLAKNYELLLAA"
     gene            complement(482..2095)
                     /gene="murJ"
                     /locus_tag="PKN92_10815"
     CDS             complement(482..2095)
                     /gene="murJ"
                     /locus_tag="PKN92_10815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNZ49327.1"
                     /translation="MEDTAQQKQAAPGEEASITRFAAIFAGGTMISRFAGLVRDIVFA
                     HTIPAVPLGSFLFAFSLPNMLRDMLGEGAVNAALVPVLSKAHASSSEEEYRKAVSSLM
                     TLMLGFFLILTAAGILVMPLAPAILSSLRTFTGKELPQSAEELQETVRLMQWTFPYLL
                     FIGAAVFAMAPLFIARRYSTPSWSPALLNFTFIGCAFLFRNQFSNPAWALVIGVWIGG
                     LIQFIVLWVDMFLHIGPVFPTFNLHYSAVSSALWLLFPVILGQAAGEVNKLVDRFFAL
                     SLGEDTVLALYISNRLVQFPLSIFGISVAVAILPALSKAYALNRDKEQCTLMTSGIRQ
                     AFFLTAPATACLIVLRKPVIRLLFQRGEFGETGTEMAASALLYAAMGLVFFSLVKVMV
                     QGFYARHNTKIPVIIASFSMLLNIVLNALLVGPMGYQGLAFATTISYAVNFSLLFFCY
                     NFLVQKLLSPALMLSLIKISAASVLMSFCVWYAHNSLDSWLGITSMIQRLVTFTGACA
                     AAALVYTFSCFFLRIDEFAALGRRLLRRK"
     gene            complement(2217..2810)
                     /locus_tag="PKN92_10820"
     CDS             complement(2217..2810)
                     /locus_tag="PKN92_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNZ49328.1"
                     /translation="MSYTIFDTPFISVVGRLWARIFLGIIGWKKEGELPDAPKFVLIA
                     APHTTNWDLPIMLALGFLLRAKLFWMSKESIFRWPFGGFLRWVGGIAIDRSKPNGVVG
                     QCIERFEQTDTLILAVPPEGTRKKVRTWKTGFYHIAVGANVPIALGYLDYKRRRGGVH
                     SLFYPTGDYEKDLAEIQKFYAGITPKYPALYESLGEK"
     gene            complement(2898..4265)
                     /locus_tag="PKN92_10825"
     CDS             complement(2898..4265)
                     /locus_tag="PKN92_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNZ49329.1"
                     /translation="MAYLLFVICFVPAEVNPKLFTLEATAHVWDVSAAELSVSGHKDI
                     LLLVNNEQAFPSCKELLVYCPDEKGDYPGTPTRSLSLPEETGALFLAEVNGQHPPELV
                     AVHGSGASIYQYGQSGFSRLDTVTFQSLFPSGSREPCFVPKAAQDLTGDGVDEWLVPV
                     AEGIQIRTAGNVITTLACDIVSEIQSGESLYITHRLPDIHSFSLEHQPTKALAFLSDE
                     FADFYWGSSWKEHKRIRLPMKLDEKWDASALMKDLTGDGFPDLVITQTRGTAQMYAET
                     QVYLATEAFTFPEVPDAVFSEKGAVSSPVVLDVNGDGKQDLVFIRIPFGVKNLVNFFV
                     RNKISIRAEVYLFDGKRFSPKADYHTNLTMDAPEGRARVAHSFGDFNGDGRMDVVYGS
                     SKNSLSIYPGDQDRFLAARSWKSFEMPAFGTVRCYKLNENEADDIIFFRPGTAEATRV
                     DVIVF"
     gene            complement(4402..5985)
                     /locus_tag="PKN92_10830"
     CDS             complement(4402..5985)
                     /locus_tag="PKN92_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ49330.1"
                     /translation="MQAAISADLIDLGELRAAVEKMPESEVLRLSLHDAVRLAVDNNP
                     DILIAELEPEKASAETYAAHGEFDPILQHTFGYTKAALSLNQQLRKFAGAFMPSAIDS
                     TSITNETTLGGKLTTGTQYAVNFNLDFEKNTYGSNVGEYGATIGLMLTQPVMRGFGKD
                     INRIRIRAGANMKALSEAQLKLVVLNKTADTIKAYWDLVGATEAVRVYEGALRNAERL
                     LTISETRRNIGTAADIDVLQAKAGVAMRQSELIQAFARVSDAGDVLKLLLNMHDNHRF
                     SSITILPLDRPDPADNAIFNTDLFDESLERSVDRALELRPENTMTDLEIANALLEEER
                     ARNDMLPQVDLVALYARGGRNTFLGRTLAGIRDNQDHTYNFGIQATIPINNRAGRGAH
                     QRARIGVRQAEERRKQAQMAMMMRVHVAARGVLTGKTLVESNHQTTLLQETNVMAEEK
                     RLKIGISNSYQVLKVQEDLTAAQVAELQANIAFEKALVELQLAEGSLLDNLGLELTPE
                     TSSIEPVSWIESVGLLYEP"
     gene            complement(6192..6656)
                     /locus_tag="PKN92_10835"
     CDS             complement(6192..6656)
                     /locus_tag="PKN92_10835"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNZ49331.1"
                     /translation="MDEMRVRTIVDEDWKARVQREKDMARKRDADASPQTPSDENAQD
                     AAEPEGDINPAFEALVSTFATQTMFCLGFIAAPGQAAPTLNLDQAKESLEVLLMVAEK
                     TRGNLTASEKVILQETLEELQRLFSARVQQVQAEAMHRAGINPSNLRGDQPS"
     gene            complement(6935..7381)
                     /gene="ndk"
                     /locus_tag="PKN92_10840"
     CDS             complement(6935..7381)
                     /gene="ndk"
                     /locus_tag="PKN92_10840"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNZ49332.1"
                     /translation="MERTFVMIKPDGVGRGLAGDVISRYEKRGLKLVALKMQHLTAAQ
                     AERHYGEHKGKSFYNELVSFITSGPSIQIVLEGKDAVAQVRKMHGATNCLDALPGTIR
                     GDFGLSNQKNLVHASDSPETAAREIALYFEEDEIISYELPIAEWLR"
     gene            7822..>8071
                     /locus_tag="PKN92_10845"
     CDS             7822..>8071
                     /locus_tag="PKN92_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HNZ49333.1"
                     /translation="MKTSASEVKEPVRCSRCIMDETVGGIRFDKDGVCNYCHMHDSMD
                     KLFPIGKAGEKILKDIAVSIRKSGKGKPYDCVVGFSGGR"
BASE COUNT         2195 a         1956 c         2019 g         1901 t
ORIGIN      
        1 tgcggcaaga agcagctcat agttttttgc taaagagata agcaatattc ctaatgatga
       61 aatgataagc cccaaaaaag tgaacagtgc ggcaccgaag cggtcggcta aaggcccgct
      121 cagaagaact ccgacgacca caccggcaaa gacaagtgct gatatccgtc ctaactgctc
      181 ttcattaagg ccgccaaatt catgcgaaaa agtcgtgtaa tggacaggca taagattaag
      241 acatatggcg acacttatca tcccgccgta gcagataatc aggagaaggg tacggttcac
      301 agtggcgctt tctgaataaa gaatgggaag agggcagccg acctccgata aaggtgcccg
      361 aacgattgcg ggatattagg cactaaaaag gtggcaagct tcaagccgaa gggggacagg
      421 cttttctttt cggcagaaaa aaaacaagcg ctggggaggg accggctcac tgaatccgga
      481 attatttgcg tcgcagcaga cgccttccaa gagcggcaaa ttcgtcgatt cgaaggaaga
      541 aacaggaaaa agtataaacc agggcagcag cagcacaggc accggtgaaa gtgacaaggc
      601 gctgtatcat acttgtaatg ccgagccagc tgtccaggct gttgtgtgca taccagacgc
      661 aaaaagacat aagtactgaa gctgcggaga tcttgatgag tgaaagcata agagcggggg
      721 atagtaactt ctgcaccaga aagttgtagc aaaagaaaag aagcgaaaaa ttaaccgcat
      781 aggagatggt cgttgcaaaa gccagaccct gatagcccat cggacctacc agtaaagcat
      841 ttaaaacaat attcaaaagc atgctgaaac tggcgataat tacaggaatt tttgtgttat
      901 ggcgcgcata aaatccttga accatcacct tcaccaggga gaagaagaca aggcccatgg
      961 cggcatacaa taaagccgac gccgccatct ctgttcccgt ctcgccaaat tcgccccgtt
     1021 gaaacagcag ccggatgacc ggcttgcgca ggacgatcag acaggcagtt gccggagccg
     1081 tcagaaagaa agcctggcgg atacccgagg tcatcagggt acattgctct ttgtcccgat
     1141 tgagcgcata ggcttttgac agtgcgggta atatggcgac agccaccgaa atgccgaaaa
     1201 tggagagagg aaactggact agccggttgg agatgtacag tgccagtacg gtgtcttctc
     1261 ccagagacaa agcaaaaaaa cgatcgacca gtttgtttac ttcaccggcg gcctgaccca
     1321 gaatgacagg aaataaaagc cataaggcac tggagacggc actgtaatgc agattgaagg
     1381 ttgggaaaac cgggccgata tgaagaaaca tgtccaccca aaggacgatg aactgaatta
     1441 agccgcctat ccatacgcca ataacaagtg cccacgcagg attgctgaat tggttgcgga
     1501 ataaaaaggc acatccgata aacgtaaaat tcagtaacgc cggtgaccac gatggggtgc
     1561 tgtaacgacg ggctataaat aacggggcca tggcaaaaac ggcagccccg ataaacagga
     1621 ggtacggaaa agtccattgc atcagacgga ccgtctcctg taattcttca gcgctttgcg
     1681 gaagttcttt tcccgtaaag gtgcgcaaag aagaaaggat agcaggcgca agaggcatga
     1741 ccagaatgcc tgctgcagtc agaatcagaa aaaatcccag cataagggtc ataagagaag
     1801 aaacggcttt gcggtattct tcctctgaac tggaggcatg tgccttgctg agtaccggaa
     1861 caagggcagc gttgaccgct ccttctccga gcatatcccg aagcatattg ggaagactga
     1921 aagcaaaaag aaaagaaccg agcggcacgg cgggaattgt atgagcgaaa acaatatccc
     1981 gtacaagccc ggcgaagcgg ctgatcatag tgccgcctgc aaaaatggca gcgaagcgag
     2041 ttatactggc ttcttcaccc ggcgcagcct gtttttgttg tgcagtatct tccaaaggga
     2101 gtcccatccg ctaagcatta atcaagtata cgatttctgt gccggcaaaa gaaatcaggt
     2161 ttttaaaaaa gaagcggcgt gtaatagaaa aaagtgatgc gtggctggat ccaacttcac
     2221 ttttcaccca ggctttcata aagcgcggga tatttgggcg ttattccagc gtaaaatttc
     2281 tggatttccg ccaaatcttt ttcgtagtcg ccggtcgggt aaaaaaggct gtgcacaccg
     2341 ccgcgccgac gtttataatc cagatagcca agagcaatgg ggacgttggc acctactgca
     2401 atatgataaa atccagtttt ccaggtacgc acttttttgc gggttccctc gggaggaaca
     2461 gccagaatta aagtatcggt ctgttcgaac cgctctatac attgaccaac aacgccattg
     2521 ggcttactcc ggtcaatggc aatgccgcca acccatcgta aaaaaccgcc gaaaggccag
     2581 cgaaaaatgg attccttgct catccagaaa agctttgccc ggagcaggaa gccaagcgcc
     2641 agcataatgg gcaaatccca gttggtcgta tggggcgcag caataagtac aaacttcggt
     2701 gcgtccggaa gttccccttc ttttttccat ccgatgatgc ccagaaaaat gcgtgcccat
     2761 aggcggccga cgacagaaat gaaaggggtg tcgaaaatgg tatatgacat gaacatctcc
     2821 taaagcgaaa acaaaaacat ccagtcaaaa agaatgttta gtattataca gtaagcagca
     2881 ggtgcttttc ccgttgacta aaacacgatg acatccacac gggtggcttc tgctgtgccc
     2941 gggcgaaaaa aaatgatatc atccgcttca ttctcattta acttatagca gcggacagta
     3001 ccgaaggcag gcatttcaaa acttttccag gaacgtgccg caagaaaccg gtcttgatcc
     3061 ccgggataaa tcgacaggct gtttttagaa ctgccataaa cgacatccat cctgccgtca
     3121 ccattaaaat caccaaaaga atgcgccacc cgtgcccgtc cttccggagc atccatggtc
     3181 aagttcgtgt gatagtcggc ttttgggctg aagcgtttac catcaaaaag atagacttct
     3241 gcacgtatgc tgattttatt gcggacaaaa aaattcacga ggtttttaac gccaaaagga
     3301 atacgtataa aaacaagatc ctgttttcca tcgccattca cgtcgagaac tacggggctc
     3361 gaaacggcac ctttttctga aaatacggcg tcagggactt ctggaaaagt aaaagcttcc
     3421 gtcgccagat acacctgagt ttccgcatac atttgtgccg tgccgcgggt ttgtgtgatc
     3481 acaagatccg gaaaaccgtc accggtaagg tctttcatta atgcggaagc gtcccatttt
     3541 tcgtccagtt tcatggggag ccggatgcgt ttatgctctt tccaactgct gccccagtaa
     3601 aaatcagcaa attcatcact cagaaatgcc agtgctttcg ttggctggtg ttccagcgaa
     3661 aaagaatgga tgtcgggcaa gcgatgagtg atataaaggc tctccccgga ttggatctcg
     3721 ctgacaatat cgcatgccag agttgtaatg acatttccag cagtgcggat ctgaatgcct
     3781 tctgcaacgg gaacaagcca ttcatccaca ccgtcaccgg ttaaatcctg tgctgctttt
     3841 ggaacaaaac agggttcccg gctgccgctg gggaagagtg attgaaaagt cactgtgtcc
     3901 aggcgggaaa aacctgattg tccgtattga taaatcgatg ctccggagcc gtgcacagct
     3961 acaagttccg gcgggtgctg tccgttaact tctgcaagaa aaagagcacc ggtctcctcc
     4021 ggcagcgaaa ggctgcgcgt tggcgtgccc ggatagtccc ccttttcatc cggacaataa
     4081 acaagaagtt ctttgcagga aggaaaagcc tgttcattat ttacgagaag cagaatatct
     4141 ttatgcccgg aaacgctgag ttcagcggct gaaacgtccc agacatgtgc cgtggcttca
     4201 agagtgaaaa gttttggatt cacttctgcg ggaacaaagc agatcacaaa gagcagatag
     4261 gccacacggt tttccttttc cgctgcttgg gatgtatcag gagaaagtgt atattgcctg
     4321 taagatatat atgccggcac tctgaaaaag agaacaggca tcagttaaga ctatgtatgc
     4381 aaaacgcgtt gctgagaaaa cttaaggctc gtagaggagc ccgacgcttt ctatccagga
     4441 aacaggctct atggaagacg tttccggggt cagctccagg cccagattgt ccagcagcga
     4501 cccttcagca agctgcagct caaccagtgc tttttcaaaa gcaatgttag cctgcaattc
     4561 cgcaacctgg gcagccgtca gatcctcctg cactttgagc acctgatagc tgtttgaaat
     4621 gccgattttc aaacgttttt cttccgccat aacattcgtt tcctgcagaa gagtggtctg
     4681 atgattactt tcaaccagtg tttttccggt aagtactccg cgggcagcta catggacgcg
     4741 catcatcatt gccatttgag cctgtttacg gcgttcttca gcttggcgca ctcctatgcg
     4801 cgcccgctgg tgagcaccgc gcccggcacg gttgttgatg gggatggttg cctgaatccc
     4861 gaagttgtag gtatgatcct gattatcccg aatgccggca agggttcggc ccagaaaggt
     4921 gttgcgccct cctctggcat atagagcaac aagatccacc tgcgggagca tatcattgcg
     4981 tgcccgctct tcttccaaaa gagcgttggc aatttcaagg tcggtcatcg tgttttccgg
     5041 gcgcagctcc agagcgcgat ccacggatct ttccagactt tcatcgaaga gatcggtatt
     5101 gaaaatggca ttgtctgcgg gatccgggcg atccagaggc agaatggtta ttgaagaaaa
     5161 acggtgatta tcatgcatat tcaggagaag ttttaaaacg tcacccgcat cagaaacgcg
     5221 cgcaaaagcc tgaataagtt ccgactgacg catggcaaca cctgcctttg cctgcagcac
     5281 atcgatgtcg gcagcggttc caatgttgcg gcgcgtttca ctgatggtca gaaggcgctc
     5341 ggcattgcga agggctccct cgtaaacacg aacagcctca gttgcgccga caagatccca
     5401 gtatgctttt atggtatcag ccgttttatt aagcacaacc agtttcagct gtgcttcaga
     5461 caatgccttc atattggcac ccgcccgaat gcggattcgg ttaatgtcct tgccgaagcc
     5521 gcgcatcaca ggctgagtga gcataagccc gatggttgcg ccatattcgc caacgttgct
     5581 gccgtaggtg tttttctcaa agtcgagatt aaaatttacg gcatactgcg tacccgtcgt
     5641 cagctttccc cccagagtgg tttcattcgt gatactggtg gaatcgatgg cggagggcat
     5701 aaatgcgccg gcaaatttcc gcagctgctg gttcagggag agcgccgctt ttgtgtaccc
     5761 gaaggtgtgc tgcaagatgg gatcaaattc gccgtgggca gcgtaggttt cagccgaggc
     5821 cttctccggc tccagctcgg caatcaaaat atccgggttg ttgtctacag ccaggcgaac
     5881 cgcatcatgc agactaagtc gcagaacttc cgattcaggc attttttcca ccgcggcgcg
     5941 taattccccc aaatcaatca ggtcggcgga aatagcggct tgcaatgcgt tggaacctgc
     6001 agtccgccct gagtcgttgg aaggggagag ggcagaacca ttatcaggta gcgcgggttc
     6061 ttcaggattc tgactgtgga ttgaaaaatg aactgcaaag caagcagcaa tcaaacaaga
     6121 aatgaaccca aagttacgca tacaatactc cctggcagcc gtgctgctga taagtaaaaa
     6181 gagggaaaga cttaagaggg ctgatcgccg cgtaagttgg aggggttaat ccctgcgcgg
     6241 tgcatcgctt ctgcctgaac ttgctggact ctggcactga ataggcgctg cagttcttcc
     6301 agcgtctcct ggagaattac tttttcactg gcagtcagat tgccgcgtgt cttttctgcg
     6361 accatcagaa gcacttccag actctctttc gcctgatcaa ggttaagcgt tggagcagcc
     6421 tgtcccgggg cggcaatgaa cccgagacaa aacatagtct gtgtggcaaa ggtgctgaca
     6481 agagcctcaa aggcagggtt gatatcgcct tcaggctcgg ctgcgtcctg agcattttca
     6541 tctgaaggag tttgaggaga ggcatctgca tcgcgcttgc gcgccatgtc tttttcacgc
     6601 tggacgcgcg ctttccaatc ctcgtcaaca attgtccgga ctctcatttc atccatgttg
     6661 tttcacctta tccgctgtgc taatttcaaa gacaataatc tgcatagcat aacacgcagc
     6721 agagaaaggg attgccgtcc ttcgatcaaa aaaacaggaa gcaaaaccgt cctgagcatt
     6781 caacaaggat tatttgtttt tatgtctgtt ctgtctggaa cgttttttgc gtttgtgctt
     6841 gttgattttg gctttgcgaa cttttcttcc acctgccacg agagaatcct cctggttaaa
     6901 ccgttgtttt caattatacg ttgtaattta cagcctaccg cagccactct gctataggca
     6961 gttcatatga aataatttcg tcttcttcaa aatacagagc aatttcacgg gctgccgttt
     7021 ctgggctatc cgatgcatgg accagatttt tctggttgct cagtccaaaa tcaccgcgaa
     7081 tggtacccgg cagtgcatcc agacaattgg ttgcaccgtg cattttccgc acctgtgcca
     7141 cagcgtcttt gccttcaaga acaatttgga ttgaagggcc cgaagtgata aaggaaacca
     7201 gttcgttata aaaagatttg cctttatgct cgccgtagtg tcgttcagcc tgcgctgcag
     7261 taagatgctg cattttaagg gcaaccaact tcaatccgcg tttttcatat cggctgataa
     7321 cgtcgcccgc aagaccacgg cccacaccat ccggctttat catgacaaat gtgcgttcca
     7381 tgaaaaatgc gtttcctttt caactattat gcagtaaaat actctttccg gatgcatggc
     7441 agaatagtat tccccccgaa gcaggcatgg cgcatgggca gaaagatata ctggcttcca
     7501 tcatccggct ggatatttat tatagcaaaa taccgttgag cacctcaagc cgttacacaa
     7561 aatctatttc agaggagcag gaaatacgca agcgctgact gccgaaaata tcccgttaac
     7621 ccgcctttcc ggttctttct tgaatttatg caatagggcg catgtttttg gtactctatt
     7681 ttccgtgctt tgtcatcgtt ttcaggttgt attcctggat tatcgactga tggcgatagc
     7741 acatacacgc aaagcgccgc aaaaatattg ttggctcttc gtgtgttcct cttttcaagg
     7801 aagacaagga caaattcacc catgaagacc tcagcctctg aagtaaaaga acctgtacgc
     7861 tgcagccgtt gcattatgga tgaaacagtt ggcggcatac gatttgataa agacggcgta
     7921 tgcaattact gccatatgca cgacagcatg gacaaactgt ttcctattgg gaaagcaggc
     7981 gagaaaatat tgaaggatat tgctgtttcc attcgtaaga gcggcaaagg gaagccctac
     8041 gactgtgttg tcggcttcag cggcggcaga g
//
LOCUS       DAOPWB010000134         8036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_8975_length_8036_cov_4.887107, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000134 DAOPWB010000000
VERSION     DAOPWB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8975_length_8036_cov_4.887107"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="PKN92_10850"
     CDS             complement(<1..924)
                     /locus_tag="PKN92_10850"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49334.1"
                     /translation="MNQVRTVLATKLRIAGHEIASVRHQSKLKVGVITIAALSLWVGG
                     YFLFQAGFRWLMLFGGTPASEFNFSDLLMTRLLSILSLTVFLLLIFSNVLVAFSTLYR
                     SKEVVYLLQGPISYEHFFYARFFECVAFSSWSLLFIGSPLMLAYGITTQAPFFFYFSL
                     AAFFLPFIIIPAAVGACLTILLVRIFPRLKTRHMVFFSIAAVGSFFAYILHVLRGARM
                     ADDQLIPAFLKATGQTQSWMLPSYWTTQGVLAATRVQYKESLFWFLMVLSTALVTLLL
                     AGRLAHYWFFPGYSFLAGQDRQRVRLREKGIL"
     gene            complement(921..1670)
                     /locus_tag="PKN92_10855"
     CDS             complement(921..1670)
                     /locus_tag="PKN92_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019392312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ49335.1"
                     /translation="MPLSIQTEKLHKHFGSNVAVNHLDLEIEPGTFFCFLGPNGAGKT
                     TTIKMLTGLLHPSSGTARLGGYDIISHPVEAKKLLGYVPDHPFLYDKLTGHEFMRFVA
                     GLYQMAEEEYRERCDRLLELFEVAHVADQLIEDYSHGMRQKLSFASCFLHRPSIMIVD
                     EPWVGLDPKNIRFVKNYLKQQCREEGLTVFMSTHTLAIAEEVADLIGIINKGTLTALG
                     TVSDIKALHNRPGSLEDVFLEITGAHTEPVS"
     gene            2855..6241
                     /gene="ileS"
                     /locus_tag="PKN92_10860"
     CDS             2855..6241
                     /gene="ileS"
                     /locus_tag="PKN92_10860"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNZ49336.1"
                     /translation="MNHECFEPIASPFSFAKAEAELRALWKTRRVYAKSLEQRRGAPR
                     FVFYEGPPTANGMPHPGHCLTRTIKDIFPRYKTMDGYLCERKAGWDTHGLPVEIEVCK
                     ELGIIDGGKAAIESYGVEGFNRACLESVFRYQREWEEMTDRIGFWVNLDDAYITYHQS
                     YVESVWWSLKQLFDRGLLYQGHKVVWWWAQGGTALSAGEVGEAYRDTDDPSITVRLAL
                     TEASKEKIGFAGQEVSLVIWTTTPWTLSSNCAACVGADIDYVLVRTSADEGREEYLVV
                     AEALLEANFGDQTEVVQRFKGADLVGLQYEPLFKYDAPEDLLTGKASEKHWEVIPGDF
                     VDLSTGTGIVHIAPAFGEDDYRICREQGIGFLCFVRPDGTFDERVTDKDPFDGTPIAG
                     QFCKDADKSILRVLKERGRILRHEQIRHSYPFCPRADKDPLIQYARRSWFIRTSAFRK
                     DFLENNSHIQWQPEHIRDGRFGNFLEGNVDWALSRERYWGTPLPVWVCEKTGNQECIA
                     SYDELLGKPGITGLDVWEKAKQDNPGLSEHLKVHKPYIDAVTYQSPWDPDARMRRVSE
                     VIDVWYDSGAMPFAQWGYPHVPGSEEVLAQNFPADFISEGIDQTRGWFYALLAISTLV
                     FDKPQPRPFRNCICLGLVHGEDGLKLSKRLKNYKDPTELFDKYSADALRWSLLAKNPP
                     TTANRLTERNVEEAQREILLRWYNVYSFFVIYANLDGFHPAGMPKALLDALGCTGVKE
                     GAAAETTAPWRNPEDRGELDRWILSELNRVVLECRSALDRFETYPATRGLTAFLDSLS
                     NWYVRRSRARFWAEAWTADKADAYWTLYECLLKFAQLMAPFTPFFADITWQNLSRPLT
                     APESVHLSPYPVSDTVLVDQQLLDAMAATRNAVNLGLSARRVENIKVRQPLGLCRIIT
                     TNPAVREALEGNLALVLEELNIKALEFAENPEAYVSYEIKPNFKLLGPRFGAKMKQAA
                     AALAKGDAAALFAQLQQTGAIDLELDGESVSLTGEEVDVRLQAKEGFAAAQGQDMVVV
                     LSTTITEALRREGWAREFIRGVQELRKEEGLAYDMRIRLFIALDDADLSAAIREHENA
                     IAGEVLAVECCYDLISGDKKHRDITIDGLTARVQIEAVQT"
     gene            complement(6262..6945)
                     /locus_tag="PKN92_10865"
     CDS             complement(6262..6945)
                     /locus_tag="PKN92_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ49337.1"
                     /translation="MDDTKNTTVPTASELRRERERAELTERIMNVARDMFVIDGYEAV
                     TLRKIAKAIEYSPAAIYQYFKDKQDLVLAIIRRDYQDLRAQLFECLKMESPLERSVEM
                     AYRYVAWGREHPNHYQLLMVPHPAWTETQSRTWAKDAPPLERDALVLLKSFVEDAIRR
                     GMLKDGFTDAAAIAATLWAGLHGLVLLEITMGTQTPFTPAINSSTFDNRFNMMIQIFK
                     EGLMKDPPR"
     gene            7521..8009
                     /locus_tag="PKN92_10870"
     CDS             7521..8009
                     /locus_tag="PKN92_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49338.1"
                     /translation="MAMFRKGMLLVAVLVLTGMLSGCQQGFLTGKWEGSVFTNGKAGF
                     TNEVREFFPNGTFVWITQTSFAGFTGTLKVTGTYKVSRNLEAPGHVDILVKSINDHTL
                     SNPYHQYGIFKMSGFLWTRQLHLIPGTSNINRPAPERLEATKGPVFIGKRKTGGLLAD
                     MF"
BASE COUNT         1936 a         1982 c         2273 g         1845 t
ORIGIN      
        1 cagaattcct ttttcacgga gccggacgcg ctgcctgtcc tgaccggcaa gaaaggaata
       61 gcccggaaag aaccagtaat gtgcaagccg ccccgcaagg agaagtgtga cgagagcggt
      121 ggagagcacc ataagaaacc agaagagact ttctttgtac tgaacccgcg tcgccgcaag
      181 aacaccctgc gttgtccagt agctcggcag catccaggac tgggtctgcc cggtggcttt
      241 cagaaaagcg gggatcagct ggtcatctgc catgcgtgca ccccggagca catgcaggat
      301 ataagcgaaa aaagagccga cggcagcgat gctgaaaaac accatatgac gggttttaag
      361 tcgagggaag atccggacga gcagaatggt caggcaggcg ccgacggcag cgggtatgat
      421 gataaaggga aggaaaaaag cggcaagtga aaaataaaaa aagaaaggtg cctgggtcgt
      481 gatgccgtag gcaagcatga gcggcgatcc gataaaaagc agcgaccagg agctgaaggc
      541 gacacactca aaaaagcggg catagaaaaa atgctcgtag ctgatgggac cctgcagcag
      601 atagaccact tctttggatc gatagagcgt ggaaaaggcg accagtacat tggagaagat
      661 caggagcaga aagacggtca ggctgagaat gctcaggagt ctggtcatga gcaggtcgct
      721 gaaattgaac tcactggcag gagtgccgcc gaagagcatg agccagcgga agcccgcctg
      781 aaaaagaaaa tagccgccta cccacagaga aagcgcggca atggtgataa cgcccacttt
      841 cagtttcgac tgatggcgca cgcttgcaat ttcgtgaccg gcgatgcgca gtttggtggc
      901 aagaacagtt ctgacctgat tcatgagacc ggctccgtgt gtgctccggt gatttcgaga
      961 aatacgtctt cgagcgagcc gggccggttg tggagcgctt tgatgtctga taccgtgccc
     1021 agagcggtca gggtgccttt attgatgata ccgatcagat cagcgacttc ctcggctatg
     1081 gcgagcgtgt gggtggacat gaataccgta agcccttctt cccggcactg ctgtttcaga
     1141 tagtttttta caaagcggat gtttttcgga tccagcccga cccagggctc atccacaatc
     1201 atgatggagg gacggtgcag gaagcaggac gcaaaagaaa gtttctgacg catgccgtgg
     1261 ctgtaatctt caatgagctg atcggcaaca tgcgccactt caaaaagttc gagaagcctg
     1321 tcgcagcgct cccggtattc ttcctccgcc atctgataca gcccggcaac aaagcgcatg
     1381 aattcatgac ccgtgagttt gtcataaaga aaaggatgat caggcacata gcccagcagt
     1441 tttttggctt ccaccggatg gctgatgatg tcgtagccac ccagtctggc agtacccgaa
     1501 cttgggtgca gaagcccggt cagcatcttg atggttgttg tttttcctgc accgttgggc
     1561 ccgaggaagc agaaaaatgt gccgggttca atttcaagat ccagatgatt gactgccacg
     1621 ttggatccga aatgcttatg cagtttttcc gtttgaatgg aaagaggcat gagtacaggt
     1681 ttccttcatt gtgaaagccg cttttatgca taatgaaaac aagtatgcca tagtgtagca
     1741 aaggggcagg ggaagatcgg aataacaact gtcttcagta gtgaaaaaag acgcaggttt
     1801 ttctgaagtg gcggatttca gaagaaagtt ttcgccatga cccgcttgcg tgccaatagg
     1861 tcgattggcg ttcccaggga tataagagca aagtgcagaa ccgaaggccg ggttatggta
     1921 tgtctctata tctcattgca tattcctaac actgtcatcc ctgcgaaggc agggatctta
     1981 ctccggatca agatccctgc cttcgcagga atgacgggga gtgcagaaat attgtcgggg
     2041 acgaggtgtc gtggagtgca ggtgttaagg cataactcag atacagatcc gggtgcctgt
     2101 ctttgtcctt atcgcttgca aatggaagaa agtgtacgtt ttcttattac ctgagactta
     2161 ccttgcccct gcaccaaaat actctggctc aaaacataaa atccgccagc ctgggaacgc
     2221 caatctccgc attggcagga gcttttgtgg tctgtgatgt tgtgtgtctg tcgcaggtca
     2281 tatttccgcg gcgaatctga gtggctgtct tcgttctgac ccgcttgtgt gccaatgcgg
     2341 agattggcgt tcccagggga agaattgcct ttcctcgtta ttcctgtttt cgcaggaatg
     2401 acaccggagg tgggcttaat gtcggcgaag gggaagaacc acgaataaac acaaatgaat
     2461 accagtcagc cgggactaat aacggcgttt tcggtcagcg cggtgctacg cctttatttt
     2521 accgttgttc ctcagtgatc cccattctgc tcgtgcaact tcatgcggct tcgtgttctt
     2581 tgtgccttcg tggctaaaga cctttgtgtc ctccggggtg aaaaataatt tgcttacccc
     2641 atacccgctg gcgtttttct tcaaaaccaa attgcgtttt actatggaat ccgcccctct
     2701 atccctcgga aaagttgatt tgttccatca caatgggaca caattagagc ctctccctat
     2761 accttttact cagtgcacgc ctgaagaata cctctttatc ttcaccaaga agagcttttt
     2821 gtaagcgttg ccagtgtcag gaaatgagac cgttatgaat cacgagtgct ttgaacccat
     2881 tgcctcaccc ttttcttttg caaaagcgga agcggagctg cgtgctctct ggaaaacccg
     2941 gcgcgtatat gcgaaaagtc ttgaacagcg ccggggcgct cctcgttttg tcttttacga
     3001 aggtcccccc accgccaatg gcatgcccca tcccgggcat tgtctgaccc gtaccatcaa
     3061 ggacatattt ccgcgttaca agaccatgga cgggtatctt tgcgagcgca aagccggttg
     3121 ggatacacac ggccttcccg tagaaatcga agtctgcaag gaactgggaa ttattgacgg
     3181 gggaaaagcc gccattgaat cctacggcgt tgaaggattc aaccgggcgt gtctggagtc
     3241 ggttttccgt tatcagcgtg aatgggagga aatgacagac cgcatcggtt tctgggtgaa
     3301 tctcgatgac gcttatatta cctatcacca gagttatgtg gaaagtgtgt ggtggtcgct
     3361 gaagcagctc tttgatcggg gtttgctgta tcaggggcat aaggtggtct ggtggtgggc
     3421 gcagggcggt actgcgctga gtgcgggtga agtgggcgag gcctatcgcg acaccgatga
     3481 tccttccatt accgtgcgtc tggcgctgac ggaagcttca aaagaaaaaa tcgggtttgc
     3541 cgggcaggaa gtttcgctgg tgatctggac gactacgccc tggacattgt cgagcaactg
     3601 cgctgcctgt gtcggtgccg acatcgacta tgtgctggtt cgcacgtcgg cggacgaagg
     3661 ccgggaagaa tatctggttg tggcggaagc gctgctggaa gccaattttg gcgatcagac
     3721 ggaagtggta cagcgcttta aaggcgcgga tctggtcggc ctgcagtatg agccgctttt
     3781 caaatacgat gcgccagaag acctgctcac aggaaaggca tcggaaaaac actgggaagt
     3841 gatccccggc gacttcgtcg acctgtccac cggtacgggc attgtgcata tcgcacctgc
     3901 cttcggtgaa gatgactaca ggatctgccg ggagcagggg atcggctttt tgtgctttgt
     3961 ccgacccgac ggaacctttg acgagcgggt gacagacaag gacccctttg acggaacacc
     4021 catagccggg cagttctgta aagacgctga taaatcgatt cttcgcgtgc tcaaagaacg
     4081 gggacggatc ctgcggcacg agcagatccg ccattcgtat cccttctgcc cccgggcgga
     4141 caaggatccg ctgatccagt atgcccggcg cagctggttc atccgtacct ccgccttcag
     4201 aaaagatttt ctggagaata acagccatat ccagtggcag cccgagcata tccgtgatgg
     4261 gcgcttcggg aattttctgg aaggcaatgt ggactgggcg ttgtccagag agcgttactg
     4321 gggcacaccg cttcctgtgt gggtttgcga gaaaaccggt aaccaggaat gcatcgcttc
     4381 ctatgatgag ctgctcggca aaccggggat tacggggctt gatgtctggg agaaagcaaa
     4441 acaggacaat cccggactga gcgagcatct caaggtgcat aagccctaca ttgatgcggt
     4501 cacttatcag agcccctggg atcccgatgc gcgtatgcgc cgggtcagcg aagtgatcga
     4561 tgtctggtac gattccggtg ccatgccctt tgcccagtgg ggctacccgc atgtgccggg
     4621 ttccgaggaa gtgctggcac agaatttccc tgctgatttc atcagcgaag ggatcgatca
     4681 gacgcggggg tggttttatg cactccttgc catcagcacg ctggtttttg ataaaccgca
     4741 gccccgtcct ttccgcaact gcatctgtct tgggctggtg catggggaag atgggctgaa
     4801 actgtcgaaa cgcctgaaga attacaagga tccgacagag ctcttcgaca aatacagtgc
     4861 cgatgcgctg cgttggagtc ttctggcgaa aaatccgccg accactgcca accgcctgac
     4921 ggagcgcaat gtggaagagg cacagcgcga gattctgttg cgctggtaca acgtctacag
     4981 cttctttgtc atctacgcca atctcgacgg tttccatcct gcaggaatgc cgaaggcgct
     5041 gcttgatgcg ctcggctgca ccggcgtgaa agagggcgca gcagcggaaa cgacggctcc
     5101 ctggcgtaat cctgaagacc ggggcgagct tgaccgctgg attctcagcg agcttaaccg
     5161 ggtcgtgctg gaatgccgct ccgcgctgga tcgctttgag acttatcctg ccacccgggg
     5221 gctgaccgca tttctggaca gtctgtcgaa ctggtatgtg cgccggagcc gtgcccgttt
     5281 ctgggcggaa gcatggacag ctgataaagc agatgcctat tggacgctct atgaatgcct
     5341 gttgaagttt gcgcagctga tggcaccctt cacgcccttc tttgccgata tcacctggca
     5401 gaatctgagc cgccctctga cggcacccga aagtgtgcac ctgtcgcctt atcccgtttc
     5461 cgatacggta ctggtagatc agcagctgct ggacgccatg gcggcaacac gcaatgcggt
     5521 caatctcgga ttaagtgccc ggcgtgttga gaatatcaag gtacgccagc cgctgggact
     5581 atgccgtatt atcacaacaa atccggcggt gcgggaggca ctggaaggca acctggcgct
     5641 ggtactggaa gagctgaata tcaaagcgct ggaatttgcg gaaaatcccg aggcttatgt
     5701 ctcttatgaa atcaagccga atttcaaact gctcggaccc cgctttggcg ctaaaatgaa
     5761 acaggcggca gcggcactgg caaaaggaga cgctgcggca ctctttgccc aactgcagca
     5821 gacaggcgca attgatctgg aacttgacgg cgaaagcgtt tcgttgaccg gagaagaggt
     5881 ggacgtccgc ctgcaggcga aagaagggtt tgcggcggcg cagggacagg atatggtggt
     5941 ggtgctgtcc acaaccatta ccgaggcact ccgccgggaa ggctgggcgc gcgagtttat
     6001 ccgcggtgtt caggaattgc gtaaggaaga aggtcttgcc tacgatatgc gcatccgtct
     6061 ttttatcgct ctggacgatg ccgacttaag cgctgccatc cgggagcatg aaaatgcgat
     6121 tgccggagag gtgcttgccg tggaatgctg ctatgacctg atcagcggag acaaaaagca
     6181 cagagacatt accattgacg ggttgactgc acgggtgcag atcgaggctg tgcagacctg
     6241 atagaaaaag aagactgcct tttaacgggg cgggtctttc atcagccctt ctttgaagat
     6301 ctgaatcatc atattgaacc gattatcaaa ggtgctggaa tttattgcag gcgtgaaagg
     6361 tgtttgggtg cccatggtga tctccagaag caccagtccg tgaagacctg cccagagtgt
     6421 agctgcaatg gcggcggcat cggtgaagcc atccttaagc atgccccggc ggatagcatc
     6481 ttcaacaaag cttttcagaa gtaccaacgc atcccgctcc agcggcgggg catcttttgc
     6541 ccaggtgcgg ctctgcgttt ctgtccacgc cgggtgagga accatcagca gctggtaatg
     6601 attgggatgt tcgcgccccc acgccacata ccggtaagcc atctccacag aacgctccag
     6661 cgggctttcc atcttcagac attcgaaaag ctgcgcccgc agatcctgat agtcccgccg
     6721 gatgatagcc agcaccagat cctgcttgtc cttgaaatat tggtaaatgg cagccggaga
     6781 atattcaatg gctttggcaa ttttccgcag ggttacggct tcgtacccgt ctataacaaa
     6841 catgtcacgg gcaacattca tgatgcgctc ggtcagttcg gcgcgttccc gctcccgccg
     6901 caattctgaa gcagtgggga cggtcgtgtt ttttgtatcg tccatgataa acgcctttca
     6961 ataaattgcc attgtactga aaatagcggc gctgtgcaag cggaatactg ctaaaaaaag
     7021 gtaatggttt actattttcc cctttaaata caggctgaaa tagagggata aagacaatta
     7081 aatgaataga aaaagggaag agtcgtctgg cataatagag acaggcagcc ggcgtcagtg
     7141 tatggacgcc ggtcagaaag tgcctgttgt acaataaaga aaggatcggc catggaagag
     7201 gcatttgttc tgttcttttt ctttcagttt ttcctgatca tgatgttcgc ggcgctcagt
     7261 tcctgagaaa cggcagaatc tgctaagcag catcaaaaat atttctctgt gagtccgacg
     7321 ttccagccgg aatccggagg acatgttcag aagcgaaaag tccagcagca ttgctgcagc
     7381 aacagtttgc ctgaaaggtc ttttctgacc cgcccggcag gtagctgttt tttgtctggc
     7441 gtgataagta ttgtggtata ctaagaatac tgtttcagtg aaccaacaac ttaaatgcca
     7501 acaaaacaag gaacaaagct atggcgatgt ttagaaaagg gatgcttctg gttgctgttc
     7561 tggtgctgac aggaatgctt tctggctgtc aacagggctt tcttaccgga aaatgggagg
     7621 gttccgtttt tacaaacggc aaagcaggct ttacaaatga ggtgcgcgag tttttcccaa
     7681 atggcacctt tgtctggatt actcaaacca gcttcgcagg ctttaccggg acgcttaaag
     7741 tgacgggcac ttacaaagtc agccgcaacc tggaggcgcc cggccatgtg gacattctgg
     7801 tcaagtccat caatgaccac acgctgagca atccttatca ccagtacggt atttttaaga
     7861 tgtccggctt tttatggaca cgccagctgc atctgattcc cggcacttcg aacataaacc
     7921 gtccggcacc tgaacggctg gaggcgacga aagggccggt tttcatcggc aaacgtaaaa
     7981 cgggcgggct ccttgccgat atgttctaaa gacgtgctgt tcggctgaaa aataag
//
LOCUS       DAOPWB010000135         8035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_8976_length_8035_cov_5.770927, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000135 DAOPWB010000000
VERSION     DAOPWB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8976_length_8035_cov_5.770927"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..2049)
                     /gene="ligA"
                     /locus_tag="PKN92_10875"
     CDS             complement(13..2049)
                     /gene="ligA"
                     /locus_tag="PKN92_10875"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNZ49339.1"
                     /translation="MANRKETAPADAAARHHELAAAIDEHSYRYYVLADPVISDREFD
                     RLLEQLKKLEEQYPDLVRPDSPTQRVGGTPVEGFTNVHHEVPMLSIDNTYNENEIRAF
                     DRRTRKLLGSEPVTYVVELKMDGVSLSLRYEAGSLVRAATRGDGATGDDVTENARTIR
                     ACPLRLRETGAMPETLEVRGEVYMTVATLAQLNEEREAEGQELFRNPRNTAAGSLKLL
                     DPRAVAKRQLQFVAYDVVTGLPESVVTHDGVLQYLKTLGFPVNPNYRHCRDIEEVIAF
                     CNHWNTARHDLGYEIDGMVIKVDLLAQHKRLGRTAKSPRWLIAYKFPAETARSVLRNI
                     EISVGKSGVFTPVAELDPVALGGSIVRRASLHNFDELQRKDLRIGDLVEVEKAGEIIP
                     QVLRFVPEARPADAVPYRIPEQCPACGSPGVKDPEGVYYRCLNTACPAQVRERLRFFA
                     GKSALDIAGMGPAVVEQLVDKGLVVNPADLYMLTLSDFMQLERLGEKSAQKLIAAIDA
                     SRKRPLNRLIVGLGIRHVGSRIASSLAEHFGSLHALAEADRAALTQVQDVGDIIADSI
                     LGYFSVAENRHMLERLEKYGLTFAAAPVQEGGTSSLLAGKSFVVTGTLISGTREEIHS
                     QIEAAGGRIASSVSKKTDFLIAGENAGSKLTKAQALGVPILTEALFKEMLENGK"
     gene            2265..3233
                     /locus_tag="PKN92_10880"
     CDS             2265..3233
                     /locus_tag="PKN92_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNZ49340.1"
                     /translation="MLDLDKISNLVEWPTIRKNIEDLFYDALCYQKTEVPELQMRLHD
                     EQEVCGVTRRLIVFNLTENELVHAWVFIPDGRDEVPAILCCHHESPMGKDEAAGIEDS
                     SQLDFALHYAERGYITLAIDMPTVNRRQTGKHEPYDTQPFYKEHKKMSFAGKVLADHV
                     RVLDMLAEIKRVDPTRVGVIGHGLGGFNALLLAACDDRIQACVSSCGFTRFAGDRYAG
                     GWAGLENLSLLPKIQKCKDGDGQFPFDWEHILALAAPTPVQIITSTCNALHDKPKSCQ
                     KAVTSAAKIYKLLGASDAIDHFMHRDGHAMAPETLEVADEWFERWL"
     gene            3286..4233
                     /locus_tag="PKN92_10885"
     CDS             3286..4233
                     /locus_tag="PKN92_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019382396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNZ49341.1"
                     /translation="MNSHPHESIQRLIDTVEQVVYGKRDVVTLCAVALLARGHLLIED
                     VPGIGKTTLAQSLAKSVKCSFARIQFTSDMLPSDVLGISILDTRSNEFVFRRGPIFAH
                     IVLADEINRTPPKTQSALLEAMSENTVSVDGITHQLPAPFMVIATQNPIEHEGTYVLP
                     ESQLDRFMLRVDIGYPPQDDELKIMRRHDPVDAIAALEPALDAEQLVALQRMVGTIHV
                     DPTLENYILQIVRATRSHEQVRLGASPRGAQALYESSQALAMISGRDYVVPDDVHTLA
                     APTLAHRLLVKSRGADLASAARERERIIREIISQIKVPV"
     gene            4236..5306
                     /locus_tag="PKN92_10890"
     CDS             4236..5306
                     /locus_tag="PKN92_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNZ49342.1"
                     /translation="MRFFSGKRYFPRFTLAGVVLAGILVVLLLAAWNTGENLLYIVFS
                     AVLGMFVLSLAAGLRVLQGLTVHRQMPHAVYRNTPFVYTMQIKNTKRFLSSFSLRLES
                     GGDAAGYLLRIPPASTVSFSAQHVLPRRGVYTLPPLEVKSAFPFGFIQYRKAFADPHE
                     ILVYPRVVLARTGGVEQSMHTAGPPRLIRGEGDEFFTIREYLPGDDMRTIAWRVTARM
                     GKLMVKETGAGHSRTLVFLLDTRWREQEGADEPFEEMIEVAASLMITLLGRQYSVGFQ
                     TPDMKVPPGRGSGQQQRILDGLARITASREPAWRDYDQEVRKLLSEPLRLFLISMNED
                     LNLAGALHQNVTALNLAEVVYG"
     gene            5299..7500
                     /locus_tag="PKN92_10895"
     CDS             5299..7500
                     /locus_tag="PKN92_10895"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNZ49343.1"
                     /translation="MDKRLHFVLRIATMFLVLAGYLALASVRRYGLAFLFFPAAFLPA
                     MPLGEWLERKSSRFRLFRKLVALAWLLFIPFSLQIYGLLDAVIFLVIFIQFYLITGEK
                     SARIYYEIYLMAFFLLLGAVVQSPEPLIAIAFFLFSVASVWAFSLVKMYHDQISAPVS
                     PLVELYRKGRKAARPERGGLLDSGFLISMTALSVLSLVITILSFLFTPRVEAGWLGRQ
                     DFRPAFTGISETVRIKGATSIYEDPSVVMHVQLPEEEDRQTAQRGGMYWRITTLSRFQ
                     EDEWSRRGLQSNYEPGVEESISRKRMFAQALGGRELVREHIADRPLVQQVIFMDEVPE
                     QGVPVFDTVMSLTVEDPGSRALVAWDGGEDFTVHLQTTGSRRLQYRAISELKLPSPDQ
                     LRASSLDYSYMDSRDFNMLTYQDLLPETLEKVRAVTASASTMYDRIQALNRWLSGEDF
                     LYSLTLPPLPEKHGIDAFILEFRRGHCELYATALALMVRSLGVPARVVSGYRGGDYSA
                     ADDAYLIRASMSHLWVEVLFPESGWVRFDPSPRSDDTLAVFGRMRMAWSLYMLRAKMF
                     WFQDVVGFQGGVRLDNFPWPWKGRSLFKKPEPHQSGAEKTQTAKETRTYFLFNPTYYG
                     TALVIAFAGIFFILYQKWRNREKLFLTSDQQRARQAYLLFLQESRRAGFETDNATPDE
                     VLEQLQGRDTVDYKAALQFVRKYEAARFGRQSCEKSFRHGVAAIFRRAPLR"
     gene            complement(7519..7839)
                     /locus_tag="PKN92_10900"
     CDS             complement(7519..7839)
                     /locus_tag="PKN92_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNZ49344.1"
                     /translation="MAWLLLTAAGILEAVWAYYMKLSEGFSRPCPIAVTLITMIASFA
                     LLSWAMKSLPLGSAYVIWTGIGAVGTFVIGIIVLGEEAGFLRIFAALLILSGLVLMKV
                     ASPQ"
     gene            complement(7952..8035)
                     /locus_tag="PKN92_10905"
     tRNA            complement(7952..8035)
                     /locus_tag="PKN92_10905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8000..8002),aa:Ser,seq:tga)
BASE COUNT         1688 a         2167 c         2246 g         1934 t
ORIGIN      
        1 gttcataacg gcttatttcc cgttttcaag catctcttta aacagagctt cggtaagaat
       61 gggtacaccc agcgcttgtg ctttggtaag ttttgatccg gcattttccc cggcaatgag
      121 aaaatctgtt tttttgctga ctgaggaggc gatgcgcccg cctgctgctt caatctggct
      181 gtgaatttct tcccgggtac cggagatcag cgttccggta acgacaaagg atttcccggc
      241 gaggagcgat gatgtgccgc cttcctgcac cggtgcggcg gcaaaggtca gcccatactt
      301 ttccagccgt tccagcatat gccggttttc agcaacactg aaatagccca ggatgctgtc
      361 agcgatgatg tcgcccacgt cctgcacctg tgtcagggcc gcccgatccg cttccgccag
      421 cgcgtgaagg cttccgaagt gctcggccag agagcttgcg atccggcttc ccacatggcg
      481 gatgcccagt cccacaatca atctgttcag cggccgtttc cgggaagcat cgatggcggc
      541 aatcagcttc tgcgccgatt tttcgcccag gcgttccagc tgcatgaaat cagatagcgt
      601 cagcatataa aggtcggcgg gatttaccac cagccctttg tcgaccagct gctccaccac
      661 tgccggaccc atacccgcaa tatccagtgc gctttttcct gcaaaaaaac gcaggcgctc
      721 gcgaacctgt gccgggcatg ccgtattcag gcatcggtag taaacgccct ccggatcttt
      781 cacgcccgga ctgccgcagg cgggacattg ttcaggtatc cggtagggga cggcatcggc
      841 cgggcgcgcc tccgggacga aacggagcac ctggggaatg atctcaccgg ctttctccac
      901 ttccaccagg tcgccgatac gcagatcctt gcgctgcagc tcgtcaaagt tgtggaggct
      961 tgcacggcgc acaatagaac cgcccaaagc tacgggatcc agttcggcaa caggcgtaaa
     1021 gacaccgctt ttgcccacgg aaatctcgat gttgcggaga acggagcggg ctgtttccgc
     1081 agggaatttg taggcgataa gccaccgcgg tgatttggca gtacgtccca ggcgcttgtg
     1141 ctgtgccagc agatccacct taatgaccat cccgtcaatt tcatagccca gatcatggcg
     1201 tgccgtgttc cagtgattgc agaaggcaat gacttcctca atatctctgc agtgccggta
     1261 attaggattt accgggaagc ccaatgtttt taaatattga agtacgccgt catgggtcac
     1321 gactgactcc ggcagcccgg tgaccacatc atacgcaaca aactgcagct gtcttttggc
     1381 aacagcccgc ggatcaagca gtttcaaact gcctgccgca gtattgcggg gattgcggaa
     1441 cagttcctgt ccctctgctt cccgctcctc gttcagctgc gccagcgtcg ccactgtcat
     1501 atagacttca ccgcgcactt ccagcgtttc aggcattgcc ccggtctctc tcagccgcag
     1561 gggacaggca cggatggtgc gcgcattttc tgtaacatcg tcgcccgttg cgccgtcgcc
     1621 gcgggttgcg gcacgcacaa gactgcccgc ttcataacgc agactgagag acacgccgtc
     1681 catcttcagt tccactacat aagtgacggg ttcgctgccc agcagtttgc gggtgcgccg
     1741 gtcaaaggca cggatttcat tttcattgta agtgttgtct atggaaagca tgggcacttc
     1801 atggtgcaca ttggtgaagc cttccacagg agtgccgccc actcgctggg tcggcgagtc
     1861 cgggcggaca agatcgggat actgttcttc cagttttttc agctgttcca gcagccggtc
     1921 aaattcccgg tcggaaatga ccgggtccgc aagcacataa taccggtaac tgtgttcatc
     1981 gattgcagct gccagttcgt gatgccttgc cgccgcatcg gcaggtgccg tttcttttct
     2041 gtttgccata gtggtttccc gggttgaaag ccgtaccttc ggaaagcaca ggaaagggct
     2101 gaaacgcctg tgcagttttc tttgtcatgt gttactcaac ttgatatgat atcaaacctg
     2161 cgagtatgtt atgacacatc ctctttgtgt taaattagtt taccatgcaa ggcaggattt
     2221 gcccaaaata aataacagaa cactcatgag tggaataaat aaacatgctt gatctcgaca
     2281 aaatttctaa cctggtcgaa tggccgacga tccggaaaaa cattgaagat ttattttatg
     2341 atgcgctctg ttatcagaag acagaagttc cggaactgca gatgcgcctt catgatgaac
     2401 aggaggtctg cggggttacc cggcgtctta tagtttttaa cctgaccgaa aacgaactgg
     2461 ttcatgcctg ggtctttata cctgatggac gtgatgaagt tcccgcaatt ctctgctgcc
     2521 atcatgaaag cccgatgggg aaagatgagg cggcggggat tgaagacagt tcccagctcg
     2581 attttgcgct gcactatgcg gaaagaggct atattacgct ggcgatagat atgcccaccg
     2641 tcaacaggcg tcagacgggc aagcacgagc cctatgatac acagccgttt tacaaagagc
     2701 ataaaaagat gagttttgcc ggaaaagtgc ttgccgatca tgtgcgcgta ctggatatgc
     2761 tggcagagat caaacgggtt gatccgaccc gtgtcggcgt gatcgggcac gggctggggg
     2821 ggttcaatgc cctgctgctt gcggcatgtg atgaccggat ccaggcctgt gtgtcgagct
     2881 gcggctttac ccgttttgcc ggtgaccgtt atgccggcgg gtgggcaggg ctggaaaact
     2941 tgagccttct tcctaaaatc cagaaatgta aagacggtga tggtcagttt ccctttgact
     3001 gggagcatat tcttgcgctt gccgcaccga ctcctgtgca aatcatcacc tccacctgca
     3061 acgcactgca cgataagccg aaaagctgcc agaaggctgt gaccagtgcc gccaaaatct
     3121 acaaactcct gggcgcttcc gacgcaatcg atcattttat gcaccgtgac ggtcatgcga
     3181 tggcgccgga aacgctggaa gtcgccgatg aatggtttga gcgctggctc tgagcgtacc
     3241 gtccctgtat aacctgcgac ataacagccc attgaaaggt tgcgtatgaa ttcccatccc
     3301 catgagagta tccagcggct gattgatacg gtggaacagg ttgtttacgg caagcgcgat
     3361 gtggtgacgc tctgcgctgt cgcccttctt gcccgcggac atcttctgat cgaagatgta
     3421 cccggcatcg gcaagacgac gctggcgcaa agtctggcga aatcggtgaa atgctccttc
     3481 gccaggatcc agtttaccag cgacatgctt ccgtccgatg tgctcggtat ttcaattctg
     3541 gatacgcgga gcaatgaatt cgtatttcgc cggggcccga tatttgccca tattgtactg
     3601 gcggatgaaa tcaaccggac gccgccgaaa acccagagtg cccttctgga ggcaatgagc
     3661 gaaaatacgg tttcggtgga cgggatcacg caccagctgc ccgccccttt tatggttatt
     3721 gcgacacaga atcccatcga acacgagggc acctatgtac tgcccgagtc gcagcttgac
     3781 cgcttcatgc tgcgcgtgga tatcggatac ccgccgcagg acgacgaact gaaaatcatg
     3841 cggcgccacg atcctgtgga tgccattgca gcactggagc cggcattgga cgcggagcag
     3901 ctggttgcgc tgcagcgcat ggtcggcaca atccatgtcg atcccactct ggaaaactat
     3961 attctccaga ttgtccgggc tacccgaagc catgaacagg tgcgtcttgg cgccagtccc
     4021 cgcggagcac aggcacttta cgaatccagc caggcgttgg cgatgatttc agggagggat
     4081 tatgtcgtgc ccgatgatgt gcacacgctg gcggcgccga cgttggcgca tcgtctgctg
     4141 gtgaagtccc ggggcgcaga tcttgcatcg gctgcccggg aacgggagcg gattatccgt
     4201 gagataatca gccagatcaa ggtgcccgta taaacatgcg ctttttttcc gggaagcgat
     4261 attttccgcg ctttaccctg gcgggtgtgg tgctggcggg aatcctggta gtcctgctgc
     4321 ttgcggcttg gaatacgggg gaaaatcttc tttacattgt tttcagtgcc gtgctgggta
     4381 tgtttgttct ctcccttgct gcagggctgc gtgtccttca ggggcttact gttcaccggc
     4441 aaatgcccca tgcggtatac cgcaacaccc cttttgtcta tacgatgcag ataaaaaaca
     4501 ccaagcgttt cctttcttcc ttttcgctgc ggctggagag cggcggagat gcggcaggct
     4561 atctgttgcg catccccccc gcttccaccg tgtcattttc ggcgcagcat gtgcttccgc
     4621 ggcggggtgt ctatacattg ccgccgctgg aagtaaaaag cgcttttcct ttcggcttta
     4681 tccagtacag aaaggctttt gcggatcccc atgagatcct cgtttatccc cgtgttgtgc
     4741 tggcacgaac gggcggtgtg gaacagagta tgcatacagc gggaccgccc cggctcatac
     4801 gcggtgaggg tgatgaattt tttaccatcc gtgagtacct ccccggtgac gatatgcgca
     4861 ccattgcctg gcgtgtcacc gcacggatgg gcaaattaat ggtaaaagaa acaggggcgg
     4921 ggcattccag aacgcttgtg tttctgctgg acacccggtg gcgcgaacag gaaggtgccg
     4981 atgagccttt tgaggagatg attgaggttg ccgcttccct tatgatcacg ctgctcgggc
     5041 ggcagtacag cgtcggcttt cagacaccgg acatgaaggt gccgccaggc agaggcagcg
     5101 ggcagcagca gcgcattctg gacggtctcg cccggatcac cgcatcacgg gaaccggcat
     5161 ggcgggatta tgatcaggaa gtgagaaaac tcctttccga gcctcttcgg ctttttctga
     5221 tttccatgaa tgaggatctg aatctcgccg gcgcactgca tcagaatgtg acggctttaa
     5281 acctcgccga ggtggtctat ggataaacgg ctccacttcg tcctgcgtat cgcaaccatg
     5341 tttctggttt tagccggata tcttgcgctg gcttcggtac gtcggtacgg cctggctttc
     5401 ctctttttcc ccgccgcttt tctgcctgcc atgccactgg gtgaatggct tgaacggaag
     5461 agcagccgct ttcggctttt tcggaaactg gttgcactgg catggcttct cttcattccc
     5521 ttttcccttc agatctacgg tcttctggat gccgtgattt ttctggtcat ttttatccag
     5581 ttttatctga ttacagggga gaagagcgcc cgaatttatt atgaaatcta tctgatggct
     5641 tttttcctgc tgctgggcgc tgtggtgcaa tcgcccgaac cgttgattgc cattgctttt
     5701 tttctctttt ctgtagcatc tgtctgggca ttttctcttg tcaagatgta tcacgatcag
     5761 atcagtgcgc cggtttcacc gctggtggaa ttgtatagga aaggtcgaaa agcagcgcgc
     5821 ccggaaagag gcggtcttct ggacagcggt tttctgatct ccatgacggc tttgtccgtt
     5881 ctgtctcttg tcattaccat cctgtccttt cttttcacgc ctcgtgtaga agcgggttgg
     5941 ctgggccgtc aggatttccg ccccgctttt acaggtattt cggaaacggt gcgcattaaa
     6001 ggcgccacct ccatctatga ggatcccagc gttgttatgc atgtgcagct gcccgaagag
     6061 gaagacaggc agacagcaca gcggggtggt atgtactggc ggatcacaac tctgtcccgc
     6121 ttccaggagg atgagtggtc acgccggggt ctgcagtcca attatgagcc gggtgtcgag
     6181 gaaagcattt cccgaaagcg tatgttcgct caggcactgg gggggcggga actggtgcgc
     6241 gaacatattg cggatagacc gctggtgcag caggtcattt ttatggatga agttccggaa
     6301 cagggggttc ctgtctttga tactgtcatg tcgctgactg tggaggatcc aggttcccgt
     6361 gcactggtag cctgggacgg cggagaggat tttacggttc atctgcagac aacaggaagc
     6421 aggcggctcc agtaccgggc aatatccgag ctcaaactgc cgtctccgga tcaattgcgg
     6481 gcttcctctc tggattacag ttatatggat tcccgtgact tcaacatgct gacctatcag
     6541 gatctgctcc cggaaacgct ggaaaaagtc cgggcagtga ccgcatccgc ttcaaccatg
     6601 tacgaccgta ttcaggcact gaaccgctgg ctcagcggcg aggactttct ctattcgctc
     6661 accttgccgc cccttccgga aaagcatggc attgatgctt tcatccttga attccggcgg
     6721 ggtcattgcg aattgtatgc gacggctctg gcgctgatgg ttcgatctct gggcgtgcct
     6781 gcccgtgtgg tttccggcta ccggggcgga gattattccg cagcagacga tgcctacctg
     6841 attcgggcga gcatgtctca tctgtgggtc gaagtacttt tcccggagag cggctgggtg
     6901 cgttttgatc cgtctccacg ctcagatgat acgctggctg ttttcggacg gatgcgtatg
     6961 gcgtggtcgc tctatatgct gcgtgcgaaa atgttctggt ttcaggacgt ggtgggtttt
     7021 cagggcggcg tccggctgga taatttcccc tggccctgga aggggcgcag tctcttcaaa
     7081 aaaccggagc cccatcagtc aggtgccgag aaaacgcaga cagcaaaaga gacgagaacc
     7141 tattttcttt ttaatcccac ctattacgga acggcgctgg ttattgcatt tgcaggcata
     7201 tttttcattc tgtaccagaa gtggaggaac cgggaaaaac tgttccttac ttccgatcag
     7261 caacgggcac gccaggcgta cctccttttt ctgcaggaaa gcaggcgtgc cggattcgaa
     7321 actgataatg ccacaccgga tgaagtgctc gaacagctcc agggccggga taccgtcgat
     7381 tacaaagcgg cgctgcagtt tgtccggaaa tatgaagcgg cacgcttcgg ccggcagtcc
     7441 tgcgaaaaat catttcgaca cggggtcgct gcaattttcc ggcgtgcccc tttgcgctga
     7501 tcagcgcctg ctccgacctt attgcgggct tgccactttc atcagcacca gtccggaaag
     7561 tatcaggagt gccgcaaaga tccggagaaa accggcttct tcgcccagca cgataatccc
     7621 tatgacaaaa gtgcccacgg cgccgatgcc tgtccagata acataagcgc tgcccagcgg
     7681 caggcttttc attgcccacg acagcagtgc aaaactggca atcatggtta tgagtgttac
     7741 cgcaatggga cagggacggg aaaagccctc cgacagcttc atgtaatagg cccagacggc
     7801 ttccagtata ccggcggcag tcagaagtaa ccaggccata agcaattcct tttcagcgac
     7861 gaacttttcc ggaatgtttt tgggaaacgg gggagcggct tcagactgcc ggagtaagag
     7921 ccgggagata gcggaacagg cagcaggaaa gtggcggagg gtgagggact cgaaccctca
     7981 aggccgaagc cgccggtttt caagaccggt gcattaccaa ttatgctaac cctcc
//
LOCUS       DAOPWB010000136         7993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_9036_length_7993_cov_5.820358, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000136 DAOPWB010000000
VERSION     DAOPWB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9036_length_7993_cov_5.820358"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3019)
                     /locus_tag="PKN92_10910"
     CDS             complement(<1..3019)
                     /locus_tag="PKN92_10910"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNZ49345.1"
                     /translation="MKNTFLRRINERPFCLDGAMGTMIQRLDLDETAFGGGDFKMLVD
                     LLCFSRARAVRDIHVSFLEAGADAVETNSFGSSPLRLAEYDFTKLQCNDFADIPEGLH
                     LPSLSVTEWAYHLSKRAAEIACDARRHYNETSDEAGRPLFVIGSMGPSNHVVSATTAN
                     LRRADFDQIAENFKAQALGLIDGGIDVFLFETQQDILELKAGVFGVKAAMAERNVDLP
                     IMAQVTVDAFSRMQIFNTDIHAAIVTMQGTGIDVFGINCSIGPDLMEKTVEKLARFSP
                     LPISVIPNAGLPVSENGKTVFRFSPEDFASFLERFVREYGVAIVGGCCGTTPEHIACA
                     AKTLRDVPLKKRTPAPGLYVSGPQKAVLLDSSENLIRFGERLNIRGSKKVRDAVENDE
                     GIRHEVLEEVVREQVQELGCPVIDVCMDSNQIDTVSALSEVAHQQTVDFQAAMCLDSF
                     QPDALAAAVKCYPGRPILNSISYEESAPGLLKMDAVIEATLGHDPVFVALCTGPKGPG
                     ATAAEKRDLADKIIRRAREKYGITEDRIFVDVNVFPIGAESDPGQNFAVETLKGIRLV
                     KEDYPNTRTILGVGNLTNGLAAKPYMRLVLTSVFLDEARKMGLDAAIINPNHYLFVSD
                     LDPTDYALAKRIILEHDMEAFETLEGIAENKKGGPKKQTVNYDTLDNERSITEKIKDG
                     FKERIPGDFDYKGHSYHYEDRIVLQMQDILDRWEPLDFVNQRLMAAMKELGDGFARGE
                     VSLPHLLRSADVMRQVMGFIEAYLQNHQKVEMPGAVRHKGVVVLGTVYQDVHSIGKDL
                     ARTLLENYGYKVIDLGVMTPLQQYIDTALEHNAQAIGMSALLVQTSNHMITVARMMKE
                     QGISLPLLVGGAPVSDRHAAYVGLAGEDDPEKMLGSVFYCRTAMDGVNVMNKIVDENT
                     GPAFYEDNRQKLIRKFKQAQKRQEVEAGLFATLPRRVIQTVRRDPAFPFFRSRVYKYP
                     LTEFAGQLDRHTLFSLNWRFGGASGWSRFGQSEK"
     gene            3018..3155
                     /locus_tag="PKN92_10915"
     CDS             3018..3155
                     /locus_tag="PKN92_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49346.1"
                     /translation="MRGVYPYTRKTPVVASKSYACPASLPVLKASVFPMKRTIRDYWS
                     A"
     gene            complement(3140..4348)
                     /locus_tag="PKN92_10920"
     CDS             complement(3140..4348)
                     /locus_tag="PKN92_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNZ49347.1"
                     /translation="MKDIPSSDPDKSNLSRRDISTLSVHAGEERFRYADSITTPIVQT
                     STYAFKNTGHIEAYTRHGEAHFEYGRYGNPTEKVAQDRLAAICGAEDCVVFGSGMSAI
                     TTTILALVKSRDHIIITDDAYKKTLEFCSAYLQRFGVDCTIVPFGDYAALEKAIRPQT
                     RFIFSESPTNPYLNIFDLERLRDLARRYNLLTLIDSTFSTPYNQCPFEYGVDIVLHSA
                     TKYLAGHNDILAGAVLGRKELIEEIRALHKAIGGVIDPHCSYLLLRGLKTFALRMERQ
                     NQSGMEVARFLENHPRVKRVYYPGLESHPQHETAKAQMTGFGSVISFDINGDLDDAKR
                     FLDKLKLCYIAPSLGGVETLITHPALVSYYSITREERYALGITDSLLRLAVGIENPAD
                     IIADLNQALQ"
     gene            4627..5988
                     /locus_tag="PKN92_10925"
     CDS             4627..5988
                     /locus_tag="PKN92_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNZ49348.1"
                     /translation="MLKQHVAFSEICLLVFVLSGLALKPGIAQQDDAGTDKVIYGNDD
                     RIDVYQENNAERRAWADAVCALVEWSDLQDNQDGTYFLKTEAYRVAGKPPCSGEAFST
                     QPMGAYCSGFMAADDIIVTAGHCLTLDRLSDFAIVFGFDMLDANTPRYLFDWSQVYFA
                     VEVLAYQYTPTLDFAVVRVDRSITAPGASPLPMRESGLVEIGTPVGVIGHPFGLPKKL
                     AFSSDTQVRRIDAPYFFFANVDAFSGNSGSPVFNAATGMVEGILVRGNTDFNVGMSCF
                     ESRRLRNDEQYPEAITRSSAFANYVYDDGLIPENDECSNATEILNDSFISGTTRNATG
                     SSVSSCGVEDYKDVWFFFQVPRNDHYLISLCDGDFDTTLALYKGSCGALDEFDCDDDS
                     CGSRSQICAELRAGETVYIRVAGKRGAVGNYILHIDSGADCSEPACGRTDSEDLAGIL
                     MFL"
     gene            6122..7507
                     /locus_tag="PKN92_10930"
     CDS             6122..7507
                     /locus_tag="PKN92_10930"
                     /inference="COORDINATES: protein motif:HMM:NF023980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HNZ49349.1"
                     /translation="MDKTILRDTQITFDAKSHSLDNNDNFSSDGKYLCYDTRESTGPG
                     IEHGFSIEVVDLTDNRCIVVYAPDEILTGATPAPGIGAVSFSQAAPEVSFIHGPPVQE
                     TKERGFYGKPNRNGACVRLSGEVVERNGSFHMLKDGEYNLSWLDKRDVHTDRDTEPGA
                     HRGGTHRHEFCMKGDRIGFTYDDFLLPQYDRTIGYMEPGPNAPAPASHYFAVLVAVAP
                     MGQAKPGELEKAYGDAWVDPEGTMRAFIGKIRKENGVDYEESLFVADMPRNVDITTAD
                     AGSASRYPAPPKGITIRRLTSTTALGIVRGAPDGKHIAYYAPDKSGVNQVFMIDAHGS
                     DQAEDPALRPVQVTSLKKGTKDGLRWTPDGKAILCISDDGLVLTSVVPGEQFGKSVFL
                     TEYGDGVNRHAPVFSPDGRKIAYNRPTATFDTSGNRVKAYNGSDYVQIFMLELADKNV
                     FSSLLDCCANK"
     gene            7530..7784
                     /locus_tag="PKN92_10935"
     CDS             7530..7784
                     /locus_tag="PKN92_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNZ49350.1"
                     /translation="MACIVIDGVAMEVADGSPIIDACEEAGVTFGCRAGLCGSCVIVI
                     ESGMENLEAMTPEEENMGLAENQRLACQAVIRSGTVVASY"
     gene            7808..>7993
                     /locus_tag="PKN92_10940"
     CDS             7808..>7993
                     /locus_tag="PKN92_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNZ49351.1"
                     /translation="MAKTALFYCEEAVAHNTGEFHPESPLRLPAILQELEKQQLTPII
                     MRPEAAAEEDILRNHTKA"
BASE COUNT         1892 a         1935 c         2156 g         2010 t
ORIGIN      
        1 ccttttcgct ctgcccgaag cgactccagc ctgatgcgcc gccgaagcgc cagttaagcg
       61 aaaaaagagt atgcctgtcc agctgcccgg caaattcggt caaagggtat ttgtaaaccc
      121 ggcttctgaa gaaagggaag gcggggtccc gacgtacagt ttgaatcacg cgacggggaa
      181 gggttgcgaa aagtccggcc tccacttcct gtcgtttttg cgcctgcttg aatttccgga
      241 tcagtttctg gcggttatcc tcgtaaaacg caggacctgt gttttcgtcc acgattttat
      301 tcattacatt tacgccgtcc atggctgtac ggcaataaaa aacagagccg agcatttttt
      361 ccggatcatc ttcgcctgcc agcccgacat aggcagcatg acggtcgctc acaggagcac
      421 cgccgacaag aaggggcagt gaaatgccct gttccttcat cattctggct acggtaatca
      481 tgtggttgga ggtctgaacc agaagcgctg acatgccgat cgcctgggcg ttatgctcca
      541 aggctgtatc aatatactgc tgcagcgggg tcatgactcc gaggtcaatc actttgtagc
      601 cgtagttttc caggagcgtg cgagccaggt ctttgccgat ggaatgtaca tcctgataga
      661 cagtgcccag caccacaact cctttgtggc gcacagcacc cggcatctcc accttctgat
      721 ggttctgcag ataggcttct ataaaaccca tcacctggcg catgacatcg gcggagcgca
      781 gaagatgggg aagagagact tcgccccggg caaaaccgtc acccaattct ttcatcgccg
      841 ccatcagacg ctggttaacg aaatcgaggg gttcccaccg atccagaata tcctgcatct
      901 gaagcactat tctgtcttca taatggtagc tgtgaccctt gtagtcaaag tcgcccggga
      961 tgcgttcctt aaaaccgtcc ttgatctttt ccgtaatgga tcgctcatta tcgagtgtgt
     1021 catagttgac tgtttgcttt ttaggaccgc cctttttgtt ttccgcaatg ccttccagcg
     1081 tctcaaaagc ctccatgtca tgctcgagaa tgatgcgttt tgcaagagca taatcagtcg
     1141 gatccagatc agaaacaaag agataatggt tgggattgat aatagcggca tcaaggccca
     1201 ttttgcgcgc ttcgtcaaga aagacagaag taagaaccag ccgcatatat ggttttgctg
     1261 caaggccgtt ggtcagattg cccactccga gaatggttcg ggtattcggg tagtcttctt
     1321 taaccagacg gatgcctttc agtgtttcca cagcaaaatt ctgtcccgga tcgctctcgg
     1381 caccgatggg aaaaacattc acatcaacaa aaatccgatc ttccgtgatg ccgtattttt
     1441 cacgggcgcg cctaatgatc ttgtcggcaa gatcacgttt ttccgctgcc gtggcaccgg
     1501 gacctttggg tcctgtgcac agggctacaa atacaggatc atgccccagc gttgcttcta
     1561 tgacggcatc cattttcagc agacccggtg cactttcttc ataagatatt gagttgagaa
     1621 tcggtctgcc cggataacat tttaccgctg ctgccagcgc atcaggctga aaggagtcca
     1681 agcacatggc ggcctgaaag tcgacggtct gctgatgtgc cacttcactc agtgccgaca
     1741 cggtatcaat ctgatttgaa tccatacata catcaatcac agggcagccc agctcctgaa
     1801 cctgttcgcg gactacttcc tcgagcactt catgcctgat gccttcatcg ttttccaccg
     1861 catcccgcac ttttttggag cctcgtatgt taagtcgctc tccgaagcgg atcaggtttt
     1921 cgctgctgtc cagcaaaacc gctttttgag ggccggaaac ataaagaccg ggagcaggcg
     1981 tacgtttttt aagaggaaca tcgcggagtg tttttgctgc gcatgcgatg tgttccgggg
     2041 tggtgccgca gcagccgccg actatggcaa cgccatactc gcgaacaaag cgttccagaa
     2101 aggatgcgaa atcttcaggg gaaaaacgga aaacggtttt gccgttttcg ctgacaggca
     2161 gaccggcatt gggaatgaca gaaatgggaa ggggcgaaaa gcgggcaagc ttttccaccg
     2221 tcttttccat aagatccgga ccgatggagc agttgattcc gaacacatcg atgcctgtac
     2281 cctgcatggt tacgatggcg gcgtgtatgt ccgtgttgaa aatctgcatg cggctgaagg
     2341 catccactgt gacctgcgcc ataatgggca ggtcaacatt gcgctctgcc attgcggctt
     2401 ttactccgaa tacgcctgcc ttcagttcca gaatgtcctg ctgggtctca aacagaaaaa
     2461 catcgatacc tccgtcaatc agtcccagtg cctgcgcttt gaagttttca gcgatctgat
     2521 caaagtcggc gcggcgcaga tttgctgttg ttgcggaaac cacgtgattc gagggtccca
     2581 tggatccgat gacaaagaga gggcgtcccg cctcatcgct cgtttcattg taatgtcttc
     2641 gtgcatcgca ggctatttcc gccgcccgtt tgctcagatg gtatgcccat tcggtcacgg
     2701 acaatgacgg aagatggagc ccttcgggaa tatcggcaaa atcgttgcat tgtaattttg
     2761 tgaaatcgta ctcagccaga cgtaaggggg aggaaccgaa agaattggtt tcgactgcat
     2821 cggcgccggc ttccaaaaaa gagacgtgga tatcccgtac cgcccgggcg cgggaaaagc
     2881 agagcaggtc gaccagcatc ttaaagtcgc cgccgccaaa tgcggtttca tccagatcca
     2941 gccgttggat catggtaccc atcgccccgt ccagacagaa aggacgttca ttaattcgcc
     3001 gaaggaaagt atttttcatg aggggtgtgt atccttatac acggaaaacg cctgttgtcg
     3061 catcaaaaag ttacgcctgt cctgcttcgt tgcctgtgct caaagccagc gtttttccga
     3121 tgaaacggac gatccgggat tattggagcg cttgattcaa gtcggcaata atatcagcgg
     3181 gattttctat ccctaccgcc aaccgaagca gggaatcagt tattcccagg gcatagcgct
     3241 cctcgcgggt gattgaataa taactgacca gcgccgggtg agtgatcagt gtttccacac
     3301 cgcccagact gggggcaatg tagcacaatt ttaatttgtc gagaaagcgt tttgcatcgt
     3361 caagatcacc gttaatatca aaggagatga cgctgccaaa cccggtcatc tgcgctttgg
     3421 ctgtttcatg ctgtgggtga ctttccagac cgggataata aacccgtttg actctgggat
     3481 ggttttccag aaaacgcgcc acttccatgc cgctttgatt ctgacgttcc atgcgcaagg
     3541 caaaagtttt aagaccgcgc agcagcaggt aggagcagtg aggatcgatt accccgccga
     3601 tcgctttatg cagcgcccga atttcctcta tcagttcttt tctgccgagc accgctccgg
     3661 caagtatgtc attatgtccg gcaagatatt ttgtggcgct gtgaagaaca atatcaacgc
     3721 cgtattcaaa cgggcactgg ttatagggtg tgctgaaggt ggagtcgatt aaagtcagca
     3781 ggttatagcg cctggcaaga tctcgcagcc gttccagatc gaaaatattc agataagggt
     3841 tggtggggga ttcgctgaag ataaagcggg tctgcgggcg gatcgctttc tccagtgcgg
     3901 catagtcacc aaaaggcact attgtgcagt cgacaccgaa gcgctgcagg tatgcgctgc
     3961 agaattccag tgtttttttg tacgcatcgt ctgtgatgat aatatggtct ctgcttttta
     4021 cgagagccag aatggtcgtg gtgatggcgc tcatgcctga gccgaacact acgcagtctt
     4081 cagcgccgca aatggcggca agcctgtcct gtgccacctt ttcggtgggg ttgccgtaac
     4141 gtccgtattc aaaatgggct tcaccgtgtc gggtgtaggc ttcaatatgc cccgtgtttt
     4201 taaaagcata ggtggaagtc tgaacgatcg gtgtggtgat tgaatccgca tagcggaatc
     4261 gctcttcacc ggcatggacg gaaagggtgg agatgtctct tcgggacaga ttagacttgt
     4321 ctggatcgga agatggtatg tctttcatgg aaacctctac ggttctcttt cacttgaaaa
     4381 aagcgtcctg atactcagga aggttttgcg tataaagccc acatgacgct ttggagcgaa
     4441 cgcacaccta tcagtataca gcctgtgcag tactgtcaca aaacagcgaa cggtgctttt
     4501 ctgaaggtcg gaaagaaaag gagaaatgtg atatgattgc aaaatcctgt gatgcatgca
     4561 ccggccgtct tcgaggcccg gttcgcctgg cacaggagag gtatttatga aggagttcat
     4621 agaaaaatgc tgaaacaaca tgttgcattt tctgaaatct gtcttcttgt gtttgttctc
     4681 tcagggcttg cccttaagcc cggaatagcc cagcaggatg atgcgggcac ggataaagtt
     4741 atctatggca atgatgaccg tatagatgtt tatcaggaaa ataatgcgga gcgtcgtgcc
     4801 tgggcggatg ctgtctgtgc gctggttgaa tggagcgatc ttcaggataa tcaggacggc
     4861 acctactttc ttaagactga agcctatcgg gttgcgggca aaccgccctg ttccggagag
     4921 gcattcagta cgcagcccat gggtgcctat tgttcaggct ttatggctgc tgacgatatt
     4981 attgtgaccg ccggtcattg tctgacactg gaccgtcttt cggactttgc aattgttttc
     5041 ggctttgata tgcttgatgc aaacacgccc cgttatcttt ttgactggag ccaggtgtat
     5101 tttgccgtgg aagtgcttgc gtaccagtat acgccgactc tggattttgc tgttgtccgt
     5161 gtagaccgga gcatcacggc gcccggtgca tcacctttgc cgatgcgtga atccgggttg
     5221 gttgaaatcg gtacgccggt gggtgttatc ggtcacccgt tcggtttgcc taaaaagctg
     5281 gcgttcagta gcgatacaca ggttcgtcgg atcgatgcgc cttatttctt ttttgccaat
     5341 gtggatgctt tcagcggaaa ctcgggttcc cctgttttta atgctgctac cgggatggtg
     5401 gagggtattc tggttcgggg aaacacagat tttaatgtag ggatgagttg ctttgaatcc
     5461 cgacggttgc gcaatgatga acaatatcct gaagccataa ccaggagtag cgcctttgcg
     5521 aattatgtgt atgacgacgg gttaattccc gaaaacgacg agtgcagcaa tgccacagaa
     5581 attctgaatg acagttttat ttcggggacg accagaaacg caaccggatc ctccgtttcc
     5641 tcctgcggcg tggaagatta taaagatgtc tggttctttt ttcaggtgcc gaggaacgac
     5701 cattacctga tcagtttatg tgatggcgat tttgatacga cactggcgct gtataagggg
     5761 tcctgcggcg cgctggatga atttgactgc gatgatgatt catgcggaag ccgctcccag
     5821 atttgcgcgg agctgcgtgc cggagagact gtctacattc gggttgccgg taaaaggggt
     5881 gctgtcggga attatatcct tcacatcgat tcgggtgccg attgttccga gcctgcctgc
     5941 ggaagaaccg acagcgaaga tttggcaggc atactcatgt ttttatgatt cttatagcaa
     6001 cgattttctt ttcctttatg aaatagattg tgcaaaagga gctgggaagg tgcgagcttc
     6061 atctcttttg tttataacgc ttctgatggt cgggtgcgtt tcaaattcaa ggatatgtac
     6121 tgtggataaa acgattctgc gggatacgca aattacattt gatgcgaaat cacattcgtt
     6181 ggataacaat gataattttt cttcagacgg aaaatacctc tgttatgata cccgggaaag
     6241 cacaggaccc ggcattgagc atggtttttc cattgaagtg gtggatctta ccgacaatag
     6301 atgcattgtt gtatatgcac ctgatgaaat cctgaccggt gccacacctg cccccggaat
     6361 tggtgccgta tccttttccc aggctgctcc ggaagtatct tttattcacg ggcctccagt
     6421 gcaggaaaca aaagagcgcg gcttttacgg aaagccgaac cgtaacggcg catgcgttcg
     6481 cctttcggga gaagtagtgg agcgcaacgg ttcctttcat atgctgaaag acggcgaata
     6541 taatttgtcc tggctggata aacgggacgt gcatacggat cgggatacag agcccggagc
     6601 acacagaggg ggtactcacc gtcacgaatt ctgcatgaaa ggcgacagaa taggatttac
     6661 ttatgatgac tttctgctgc ctcaatatga ccgcaccatc gggtatatgg agcccggtcc
     6721 caatgcgcct gctcctgcca gccattattt tgctgtgctg gtcgctgtgg cgccgatggg
     6781 tcaggcgaag cccggtgagc tggaaaaagc ctatggcgat gcctgggtgg accccgaggg
     6841 taccatgcgt gcttttatcg gaaagattcg caaagaaaac ggcgtcgatt atgaagaatc
     6901 gctctttgta gcagatatgc cgcgaaatgt ggacattact acagccgatg ccggcagtgc
     6961 ttcccgttat cctgcgcctc caaaagggat cacgatccgc aggctgactt ccacaactgc
     7021 cctgggtatt gtgagaggag cgccggacgg aaagcatatt gcctattatg ccccggataa
     7081 gtccggcgtg aatcaggtgt ttatgattga tgcccacggc agcgatcagg cggaggatcc
     7141 tgcgctgcgg cctgtacagg tgacttcgct gaaaaaagga acaaaagacg gattacgttg
     7201 gacgccggac ggtaaagcga ttctttgcat atcggacgac ggcctcgtgc tgacttctgt
     7261 agtgccggga gaacagttcg gtaaatcagt ttttctgaca gagtacggag acggagtgaa
     7321 caggcacgcg cccgtatttt cgccggacgg cagaaaaata gcgtataatc ggccaacagc
     7381 cacctttgat acttccggga acagggtaaa agcctataac ggcagtgact atgtgcaaat
     7441 cttcatgctg gagctggcag acaaaaatgt gttctccagc cttttggatt gttgtgcaaa
     7501 taaataattc cacaggaaag ggataaacaa tggcgtgtat tgtaattgac ggtgtcgcaa
     7561 tggaagttgc cgacggatcc cctattattg atgcctgcga agaggcaggt gtgaccttcg
     7621 gctgccgtgc aggcctctgc ggcagctgcg tgatcgttat agaatcgggt atggaaaatc
     7681 tggaagcgat gactccggaa gaagaaaata tgggactggc ggagaatcag cggctggcat
     7741 gccaggccgt gatccgctca ggaacggttg tggcctctta ctgatcctga ttcgaaagga
     7801 caatcttatg gcaaagaccg ccctgttcta ctgtgaggaa gcagtggcac ataatacagg
     7861 tgagttccat cctgagtcgc ctctgcgtct gccggccatt ttgcaggaac tggaaaaaca
     7921 gcagctgacg ccgatcatca tgcgcccgga ggcggcggct gaagaagaca ttctccgcaa
     7981 ccatacaaaa gcc
//
LOCUS       DAOPWB010000137         7781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_9343_length_7781_cov_4.445509, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000137 DAOPWB010000000
VERSION     DAOPWB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9343_length_7781_cov_4.445509"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKN92_10945"
     CDS             complement(<1..288)
                     /locus_tag="PKN92_10945"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNZ49352.1"
                     /translation="MRYRQPCRAVIGGHILHVFRHAFVSNADNLGALLHLGILGHVAA
                     RRIPFLMEVTARTECDRKGGHLARDRRTGRLLLRESAQCPAAETADFQDIAK"
     gene            340..1050
                     /locus_tag="PKN92_10950"
     CDS             340..1050
                     /locus_tag="PKN92_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49353.1"
                     /translation="MAINTDIHVKLFTESGLKELNELALTGTLEKKNSFGYMTRVPLP
                     PFDRACEERINADVRFRVIVPTSGLSSVDPDNIRIFWKFYRDGALVASGLAQDNWAES
                     ARPFNLLVDLLRHDAVIVQNTEEMNTPPANLSNAWACMLPRDRFLARRPDACMYYNQL
                     FTRLGDEGNPPLKENINADSNRRNEIFYTLTAQTVYKDASGYLFMDGTPARASFSIYA
                     SEAESMKLQDTPVREFSR"
     gene            1117..7740
                     /locus_tag="PKN92_10955"
     CDS             1117..7740
                     /locus_tag="PKN92_10955"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNZ49354.1"
                     /translation="MLYKKNHFAFVSCAIALLIFSIAIIDNHASAQVSAIPWQDGWSA
                     NGPVHAIEPSSDAVYIGGAFTYVGQQTGNLVRLDGTTHLFDRFCPKVQGIVYAAVPDG
                     KGGWYFGGAFSVAGGERHAGLAHMDADGILYTDPLPGVNVQGGEAIYALALSADGQVL
                     FIGGKFDSIGGVNRRNLAALNTATGAVHVWQPDPDGFVRVFAQSHDKKLLYVGGNFEA
                     SKLERNQPYLFSVHADPASANFGALTTWDPEADGAVETLFYTKAIDGEFEEPEDPDNA
                     EDQYVLYVGGWFSTLGNSHKSFLGAVDADTAVARNWNPAPDNPVFALAVDDKTVYAGG
                     SFNRIGFRKCSFLGAVDRVSGQVPDSWLLREDTEKLNGWVTALALSTTPDLLYAGGHF
                     TRIGSKVRARLAKINCDAESSTYGAPTGWDPGAGDITYVLSPYVDPVAGEEKVLVAGK
                     ATEVNGKIRHRLAAFDKGATPGPDYVLNDWNPHANNPVYALAVSEGDAVVYAGGIFTS
                     MGDTGNPEVYAQERKRLAAIHDDRGTNYGKLIDAWTPEADRTVYALSYTDALALSPKL
                     LYMGGAFTKIDEQVRPGLAAVNASAGDADYGSLINWQPGLPAVYYALALSDDNDFIFA
                     GGEEADYDGLVACINAGGSPVWTLEADLAVRSLASAPGDVLYAGGFFNEIKNASNVHT
                     CMHLARIDDSVANGIVRTDWRPGVNGPVYSLLPAFHGVYLAAGGNFSHSVPQAQEEAL
                     LLGNLAVIQSDPVLPEFGQFFSLLPKADQPVYVLTHDSIKPFLFAGGAFSSMSVDGIH
                     YYLANALAIFLMNDVTPPVITLKGDAALTLECGTVYTELGATALDDWDGNISYALIID
                     ATAVDTSVPGDYKVIYSVQDSAGNASTEERDVKVQDTIAPEITLLGDAEIRLQCNADT
                     FVDPGAVVVDSCDGVIPYVVNGNLDLSQPGVYQLFYEAVSGDTAGNTVGPLTRTVIVE
                     DTYPPVFEMTELPVINIETGQTFTDQIPFAFDQCRGVVPVTSDAAAVVRADQPGVYTV
                     TYTASDGQNTATITRTVYVTALDKPHVGAVTVPSTSSILVVFSSPMSSQALMPAYYTL
                     SGPGRGSFGAHPDRVLKDPSEPGAYILEWNSPAEMLDGGSLILTVNPLMTDADGNLLD
                     EPKFGSAVGKGLPPTITVLGNNPETIECGVAYSDAGATAFDVPAGTIQVTVTGLDALV
                     GAPTGTYEVVYQATDPAGNTSIEKRQIVVEDTEAPTLTLVGDAEMTINCGTAFADPGA
                     TAEDDCEGTLTSQVTVSYFYENGNPVDTLNGAAPGIYRAHYNVSDSAANKAQAVERKI
                     VVKSAFYLYIGSVFTEPPVFQNGFQVWECSRDFIEPVDFQASDLCTGDLGRPNSGLQP
                     GEAGVKAFAWALDAAKQPVVQNGIPLVYSADAYIRHPGDYLLFYVAYDAHGDSYPTLN
                     ALGAPTLPSPLYLDAQLEVLAIDYGRLVRIVDSVAPQISLGSTDPMVLECGTLFQDSV
                     YAFDDCNGDISSLVTASGTVNISVPGSYTIAYTVTDSSGNAAVPVWRTVSVVDTAPPE
                     ILLNGPATLQVDCGSSFSSPVTAIDACDGDLTAALTVTGTVNTNIPGTYTLGYTVADS
                     SGNAAVPTYLTVIVSDMTPPVLSVNGPDSILIECHDSYSFDVTAFDNCDGDLTSTITV
                     SGFVNVGVPGNYQLTYAVTDSAGNAAVPRICMVKVQDHELPVIHLNGPDTVIVQCGVT
                     WTDPVTATDSCAGDLTAMIQALPVVDTAIPGIFNITYTVADPSGNTDMATRTVIVEDT
                     IPPVIHAGSNYGPTAEIVGGFLKWYQGNTFVEPSDFTVFDACEGVFSAIAPLMPPSTA
                     PLIAYAWALDPFTKEPLWNTPMTVPLWYSYDDFVNHPGYYLVVYEAQDSTGNHTPPPG
                     VGGFVPIFDGSFLPNFLEAGGDLRADITFARLVWVTMNKMDELPFDFASWVWGSFGTW
                     DANGDSLLDKSEQEEALNAYADAHPGVAAGELLFIAGQLAENQMLTAALLEDFIRKHE
                     APCDPDETAPVVFTDAITLSLGEDGTVSLFDETQPYGTAAVRYLTDNCSSFEKGELTI
                     TAEPDLFDCANLGANTVILSVTDQAGGKTEKTLQVVVEDPAHFCASPTEGESVVEGET
                     QPEGEVQPEGEVVTEGETQSEGEIDQPEDEDAAVGCCRSTESRAYDVRQLFGDWLLLG
                     LSVMALLLLAGVGRGKS"
BASE COUNT         1720 a         1957 c         2196 g         1908 t
ORIGIN      
        1 cttcgcgatg tcttggaaat cggcggtctc ggcggcgggg cattgcgcgg attcgcgcag
       61 cagcaggcgg ccggtccggc ggtcgcgggc gaggtgcccg cccttgcggt cgcactcggt
      121 gcgggcggtg acctccatca ggaagggaat gcggcgggcg gcgacgtgcc cgaggatgcc
      181 caggtgcagc agggcgccga ggttgtcggc gttggagacg aaggcgtggc ggaatacgtg
      241 cagtatgtgc ccgccaatta cagctctgca gggctgacgg tagcgcatct gtccgctgcc
      301 ggtattgact atgttttgtg ggtgcatggc gttgagaatg tggcaattaa cacggatatt
      361 catgtaaagc tgtttactga atccggtctt aaagaactga acgaactggc attgacaggt
      421 acgctcgaga aaaaaaattc tttcggttat atgacccgag tgccgcttcc tccttttgat
      481 cgggcgtgcg aagagcgcat caatgcggat gtgcgcttcc gtgtgatcgt gccgacttcc
      541 ggtctctctt cggtggatcc ggacaatata cggatcttct ggaagtttta ccgcgatggc
      601 gctctggtgg cttcaggact ggctcaagac aactgggctg agtccgcccg tccgtttaac
      661 cttcttgtcg atttgctccg gcacgatgcc gttattgtgc aaaatacaga ggaaatgaat
      721 acacctcctg caaatctgtc gaatgcctgg gcatgcatgc ttccgcgcga ccgtttttta
      781 gcgcgccgtc cggatgcctg tatgtactac aaccaactct tcacacgcct gggtgacgaa
      841 ggcaatccgc cgttgaaaga aaatatcaat gctgattcaa atcgccgcaa tgagattttc
      901 tatacgctga ctgcacagac cgtatacaaa gacgcatcgg gatatctgtt tatggatggc
      961 acaccggccc gtgccagttt ctccatttat gccagtgaag cagagagcat gaaactccag
     1021 gatactccgg tacgcgaatt cagccgataa gagcaggctt ttatcctgat gtggcgtccc
     1081 tgaaaatact taagcaataa ggagcagcaa cagagaatgt tgtataaaaa aaatcacttt
     1141 gcttttgtct cgtgtgctat cgctctgctt atttttagca tagccatcat cgacaaccat
     1201 gcatctgctc aagtaagcgc cattccctgg caggatggct ggtctgccaa tggaccggtt
     1261 catgccatag agccctcttc ggatgccgta tatatcggcg gagccttcac gtacgtgggg
     1321 cagcagacgg ggaatctggt gcgcctcgac ggaacgaccc atctttttga caggttttgt
     1381 cccaaagtgc agggtattgt ttatgccgct gtacctgatg gaaagggcgg atggtatttc
     1441 ggaggcgctt tttccgtagc gggcggtgag cgccacgccg ggcttgccca catggatgca
     1501 gacggtatcc tctataccga cccgttgccc ggggtcaatg tccagggcgg agaagctatt
     1561 tatgcacttg cactgtcagc agacgggcag gtgcttttta ttggaggcaa atttgactca
     1621 attggaggcg tgaaccgaag aaatcttgca gctttaaata ccgcaaccgg tgccgtgcat
     1681 gtctggcagc ccgatccgga cggctttgtc cgggtctttg cccagtcgca cgacaagaaa
     1741 ctgctgtatg tgggcggtaa ctttgaagcg tcaaagttgg aaagaaatca gccatatctt
     1801 ttttctgtgc atgcagatcc tgcatcggca aattttggtg cgctgaccac ctgggatccg
     1861 gaagcggatg gtgctgtaga aacactcttt tatacaaaag cgatagacgg agagtttgaa
     1921 gagccggaag atcctgacaa cgccgaggat cagtatgttc tttatgtcgg cgggtggttt
     1981 tccacgctcg gcaacagcca caagagtttt ctgggagcgg tcgatgccga caccgctgtc
     2041 gcgcgcaatt ggaatccagc tccggacaac cctgtttttg ctcttgccgt tgacgacaaa
     2101 acggtttatg ccggagggtc ttttaaccgt attggtttca ggaaatgttc ttttctcgga
     2161 gcagtggatc gtgtttccgg acaggttccg gacagctggc tcctgcggga agatactgaa
     2221 aaacttaatg gatgggtgac cgctcttgct ttgtcaacca ctccggatct gctttatgca
     2281 ggggggcatt ttacccgtat cggatccaaa gtccgtgcac gactggcaaa aataaactgt
     2341 gatgcggaat ccagtactta cggagcaccg accggatggg atcccggcgc gggcgatata
     2401 acttatgtgc tctctccgta tgtggatccg gttgccgggg aagaaaaggt gctggtggca
     2461 ggcaaggcga ccgaggtcaa tggaaagatt cgccaccgcc ttgccgcctt tgataaaggt
     2521 gcaacgcctg ggccggacta tgttctgaat gattggaatc ctcatgccaa caatccggtt
     2581 tacgcactgg ctgtctctga aggagatgcg gttgtttatg ccggaggcat atttacttcc
     2641 atgggtgata caggcaatcc ggaggtgtat gcccaggagc ggaaacggct ggctgccatt
     2701 cacgacgacc gtgggaccaa ttacggaaaa ctcatcgatg cgtggacgcc tgaagcagat
     2761 cgcacagtat atgccctttc gtatacagat gcattggctt tatcgccgaa gctgctgtat
     2821 atgggcgggg cttttacgaa aatagacgaa caggtgcgtc cgggtctggc ggcagttaac
     2881 gcctctgcag gcgatgcgga ctatggcagt ctaatcaact ggcagccggg tctgcccgcc
     2941 gtatactatg cgctggcttt gtcggacgat aatgatttca tatttgcagg cggtgaagag
     3001 gcggattatg acgggctggt cgcctgtatc aatgcgggcg gatctcccgt atggacactc
     3061 gaggcggatc tggcggtacg tagcctggct tctgcaccag gtgatgttct gtatgcaggc
     3121 ggttttttca atgaaattaa aaatgcatcc aatgtgcata cttgtatgca tctggcgcgc
     3181 atagatgatt ccgttgcgaa cggcatcgtc agaactgact ggaggccggg agttaacggc
     3241 cccgtctatt cattgctgcc ggcttttcac ggggtttatc ttgccgccgg cggtaacttt
     3301 tctcacagcg tcccgcaagc acaggaagag gcgttgctgc tgggtaattt ggctgtgatt
     3361 cagtcagacc cggtgcttcc cgagttcgga cagttctttt ccctccttcc caaagcggat
     3421 cagcctgtgt atgtactgac ccatgacagc ataaagccct tcttatttgc cggcggtgct
     3481 ttcagttcca tgagtgtgga cggcatacat tattatctcg ccaacgcact ggcgattttt
     3541 cttatgaatg atgtcacgcc gccggtaatt acactcaaag gagatgcagc gcttacgctg
     3601 gaatgcggca ccgtctatac agagctcgga gcgacggctc tggatgattg ggacggcaat
     3661 atcagctatg cgctcattat cgacgccacc gcagtcgaca catccgtgcc aggcgattat
     3721 aaagttattt attccgtaca ggacagtgcc ggcaatgcat caactgaaga acgtgatgta
     3781 aaagtgcagg ataccatcgc accggaaata acacttctgg gagatgcgga aatcaggctt
     3841 cagtgcaatg cggatacttt tgtcgatccg ggcgcagtcg tggttgacag ctgcgatggc
     3901 gtaattcctt atgttgtgaa cggaaatctg gatctttccc agcccggcgt ttaccagttg
     3961 ttctatgaag cagtgtccgg tgacacggca ggaaatacgg tgggacctct cacccgaact
     4021 gttattgtcg aggatacata cccgcctgtg tttgaaatga cggaacttcc tgttatcaac
     4081 attgaaacgg gacagacctt tacagatcag atcccatttg cttttgacca gtgccggggt
     4141 gttgttcccg taaccagtga tgccgctgcg gttgttcggg cagatcaacc cggtgtctat
     4201 acagtgacct atacagcctc ggatggacag aatacagcga caattacaag aactgtttat
     4261 gtgactgctt tggacaaacc ccatgtgggc gctgtcactg ttccctcgac atccagtatt
     4321 ctggttgttt tcagcagtcc tatgagcagt caggcattga tgcctgctta ttataccctc
     4381 agcggtcccg gtcgtggctc tttcggtgcc cacccggatc gtgtgctgaa ggatccgtca
     4441 gagcccggtg cgtatattct ggaatggaac agcccggcgg aaatgctcga tggcggctcg
     4501 ctgattctga cagtgaaccc gctcatgacc gatgccgacg gcaatcttct ggatgagccg
     4561 aaatttggat ccgctgtggg gaaagggctt cctccgacca ttactgtgct ggggaacaat
     4621 cctgaaacca tagaatgcgg tgttgcgtac agcgatgccg gggcaacggc gtttgatgtt
     4681 cccgccggta ccatacaagt gactgttacg ggactggacg ctctggtagg ggcgccgacc
     4741 ggtacctatg aggtcgtata tcaggcgacg gatccggcag gcaacacaag cattgagaaa
     4801 cggcagattg tcgtggaaga tacagaagcc ccgacgctca cacttgtcgg tgatgccgaa
     4861 atgacgatca attgcggtac tgcttttgct gatcccggcg caacggcgga agatgactgc
     4921 gaagggacgc tgacatcgca ggtgactgtc tcgtattttt atgaaaacgg caatcctgtg
     4981 gataccctga atggagctgc cccgggaata taccgcgcac actataatgt cagcgacagc
     5041 gcagccaata aggctcaggc ggtggagcgg aaaattgtgg tgaagagcgc cttctacctg
     5101 tatataggct ctgttttcac cgaaccgccc gttttccaga acggcttcca ggtctgggag
     5161 tgctccagag attttattga gcctgttgat tttcaggcaa gcgatctgtg taccggtgat
     5221 ctcggcaggc ccaattccgg attgcagccg ggagaggctg gggtcaaagc ctttgcctgg
     5281 gcgctggatg ctgcaaaaca gcccgttgtg cagaatggca ttcctcttgt ctattctgcc
     5341 gacgcttaca tcagacatcc cggagactat ctccttttct atgttgctta cgatgcgcac
     5401 ggagacagtt atcccacctt gaacgcactg ggtgctccga cgcttccgtc gcctctttat
     5461 ctggatgcac agctggaagt actggcaatt gactacggaa ggcttgtgcg tattgtagac
     5521 agtgttgcgc ctcaaatttc tcttggtagt acagatccta tggttctgga atgcgggaca
     5581 cttttccagg attctgtcta cgcctttgac gattgcaatg gagacataag cagccttgtc
     5641 accgcttcag gcactgtaaa catatcggtg ccgggctcct atacgattgc gtacacagtg
     5701 accgacagca gcggcaatgc agccgtgccc gtgtggcgta ccgtctcggt agtggatacc
     5761 gcacctccgg agatactgct caatgggccc gccacactgc aggtggattg cggaagctct
     5821 ttcagcagcc ccgttactgc gatagatgcc tgtgacggcg atttgacggc ggctctgacg
     5881 gtcaccggta ctgtaaacac gaacattcct ggaacctata cgctcggtta tacagtcgcc
     5941 gacagcagcg gcaatgctgc cgtgccgaca tatctgactg tcattgtttc ggatatgacg
     6001 ccgccggtgc tttctgttaa cggaccggac tctatcctta tcgaatgcca tgattcttac
     6061 agtttcgatg tcactgcatt tgacaactgt gacggagatc tgacctcgac aatcactgta
     6121 agcggatttg taaatgttgg agttccaggc aattaccaac tcacctacgc tgttacagac
     6181 agcgcgggca atgcggcggt accccgtatt tgcatggtga aagtacagga tcatgaactg
     6241 cctgttattc atctgaacgg tccggatacc gtcattgtcc aatgcggagt gacctggact
     6301 gatccggtta cagccacgga cagctgtgcc ggcgacctta cagcgatgat ccaggcgttg
     6361 cctgttgtgg atactgccat tcccggtatt tttaacatca cctatactgt tgcagatccc
     6421 tccggaaaca cggatatggc aacacgcaca gtgattgtgg aagacacaat accgcctgta
     6481 attcatgccg gttcaaatta tgggccgact gctgaaatag taggtggttt cctgaaatgg
     6541 tatcagggca atacttttgt tgagcccagt gactttactg tttttgatgc ctgtgagggc
     6601 gttttttcgg ccattgcgcc gctgatgccc ccttccacag caccgctgat tgcctatgcc
     6661 tgggcccttg atccgttcac aaaagagcct ctgtggaata cgcctatgac agtgcctctc
     6721 tggtactctt acgatgactt tgtaaatcat ccggggtatt atctggtcgt ttacgaagca
     6781 caggacagta ccggcaacca cacgccaccg ccaggcgtgg gcggctttgt gcctatcttt
     6841 gacggctctt tcctgccgaa cttcctggaa gcaggcggag atctgcgggc tgatattact
     6901 tttgcccggc tggtctgggt taccatgaac aaaatggatg aattgccctt tgattttgcg
     6961 tcctgggtgt ggggaagttt cggcacctgg gatgccaacg gcgacagtct gcttgataag
     7021 tcggagcagg aggaagcact caacgcctat gccgatgcac accctggggt tgcggcgggc
     7081 gaacttttgt ttattgcagg tcaactggca gaaaaccaga tgctgacggc ggcgctgctg
     7141 gaagacttta tcagaaaaca cgaggcgccc tgtgatccgg acgaaaccgc acctgtagtc
     7201 tttactgacg cgataacgct atctcttggt gaagacggca ccgtatctct ctttgacgag
     7261 acgcagcctt atggtacagc agctgtgcgc tatctgacgg acaactgcag ttcctttgaa
     7321 aaaggcgaac tgacgattac cgccgagccg gatctttttg attgtgcgaa cctcggcgca
     7381 aataccgtga ttctttctgt cacggatcag gcaggcggca agacagaaaa aacactgcag
     7441 gttgtggttg aagaccctgc ccatttctgt gcctctccga cagagggtga gagtgtggtc
     7501 gaaggcgaaa cacaacccga aggtgaagta cagcctgaag gagaggtcgt gaccgaaggc
     7561 gaaacacaat ccgaaggtga gatcgaccaa ccggaagatg aagatgcagc agtgggctgc
     7621 tgccgatcca cagaaagccg ggcgtatgac gtgcgtcagc tctttggcga ctggctgctt
     7681 ctggggctta gcgtgatggc gcttctgctg ctggctggtg tcggcagggg aaaaagctga
     7741 cctgattctt tccgggctta tttagcacaa acgctgcggt a
//
LOCUS       DAOPWB010000138         7773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_9358_length_7773_cov_4.876522, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000138 DAOPWB010000000
VERSION     DAOPWB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9358_length_7773_cov_4.876522"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PKN92_10960"
     CDS             <1..838
                     /locus_tag="PKN92_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49355.1"
                     /translation="PVTVVSAIAGAAFLQAAHITREEHWLNTARNISVFFQKDLPRLP
                     GEDSFFCFAYAPGDTRRIHNANLLAIEHILHTARLSEEIGTAEALLPALHFSLAAQRE
                     DGAWPYGVREPGDPFEPRLMRHVDNHHTGFVLRSLHGIDCAVPGLVPELDAVIRRGYR
                     FYRTLFSTVGRPLEEGRRWPVDIHAAAEGILCPSVLAERFRGTANDALAVLRWTHEHM
                     RDAATSAPWYRLYPFFASRILYPRWSLAWMFRALAEYLYATQDNGPSIQQHYNLSRSS
                     KV"
     gene            complement(808..3021)
                     /locus_tag="PKN92_10965"
     CDS             complement(808..3021)
                     /locus_tag="PKN92_10965"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNZ49356.1"
                     /translation="MQPHDYLMSALSRAVADAQAPGAVACVGENGRTIFKGATGFRAL
                     VPEKERADTETIYDLASLTKVIATTTSVLLLREQAQLDLDDALYRYLPLANLDKVSIR
                     QVLTHTAGLVSWKAFYTELSGPLAYTDAIARLAASRDQRGTRVYSDLGFILLTRVIEE
                     ITHESFDVFCREQIFQPLKMVNTFFKPAPSFKERCAPTEKCSWRKRLIRGEVHDQNAF
                     AMGGVSGHAGLFSTAADLELFCQALLSGKILSEATLTDITREGQVPGYPWQGLGWWID
                     PWTSGANGYLPSRTAFGHTGWTGTSMWMDRSTGVYAVLLSNTCHPDVSHRNNGALRRT
                     FYSKVASLFYKNSANAHTGLDALVRNDFEEIKGKRLGVLTNSAAQDQTGRSAALVLGQ
                     SGAATVMRFFSPEHGFQGEAEAGEAVGSKKDGTVPVISLYGGKSAPDRKDLQGIDLFV
                     VDLPDIGARYYTYMATMKECMASCAEAQIPVLVLDRPNPLGGLVLEGKPAEEFGTAVC
                     CAPVPVRHGMTLGEVALFFQEQFFKGKKLELRIVKAENWWYDFTHPESALPWTPPSPN
                     IPTPDTALLYIGTCLFEGVNMNEGRGTERPFRICGAPWLKAEEVIAAVEPSALEGCTL
                     KPALYIPKSIPGKAANPEYKDQLCHGIEFEITDRRTIRPFAMVTAVLSAIHKRHPELQ
                     WRPFFDVLAGGPYLRTALQGGLPVNGILESMRESLEDFSLRRPRLYQTLEERERL"
     gene            3159..3548
                     /locus_tag="PKN92_10970"
     CDS             3159..3548
                     /locus_tag="PKN92_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49357.1"
                     /translation="MLLALHLHGYFFAGAFFTTAFLAAGFAAGFAAAFLTAGFAAGFA
                     AAFLTTGFAAGFAAGFAAAFLTAGFAAGFAAAFLTAGFAAGFAAAFSATFAAGFAAAF
                     FTAAFLAGAFFSEAFFSTIWLLLLVKP"
     gene            complement(3998..5725)
                     /locus_tag="PKN92_10975"
     CDS             complement(3998..5725)
                     /locus_tag="PKN92_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNZ49358.1"
                     /translation="MTTVDWRLYWHSLHFLSWRQIVFRLFRPLRRGRARFSLLCPFRK
                     AWFDPVYPDTLRTLLSTAAEKGATERISLDALRKFQFHYLNRTFQEDEVLLWQRQDLP
                     KLWLYHLHGFRGAREFSMAPLLTPHLGDRDRVLVWMHNWIRHNPPGRGVGWDGWPLSE
                     RLISWPVLLAVYHIKDKDIIQSYAQQARWLYHTAEHDLQGNHLLKNALALTLAGSLLE
                     DSRICRYGTALLKAELEKQILPDGGHVERSLLYHNELLWDSLIVLSLLQEAPSFLVSA
                     IRKMASFTRVLCHPDGDIPLFGDASLNESPLPQVLCACADVLTDTACTVDIQRTDCSL
                     ACPASGYFLLGDFCAGSVMIVKTAAPSPAHQAAHSHGDMLSYELSLSGKRIIVDTGTH
                     GYAESPYRAHCRSTSAHNVLTPEGREQAEHWSNFRMARRAEALPASLQKTEKGCRFAG
                     AVKYYQGGVYHRRIEFDAQSVCWEICDYLSPDCLDGSPISHIHFHPACTVHWHEPDRE
                     LLIIRDKVRVLLTIRNIEYIESSQLEMCSYFPKFGVMQSGTVLTLLGRPGEAGSRAYQ
                     IRWLPNLES"
     gene            complement(5722..6873)
                     /locus_tag="PKN92_10980"
     CDS             complement(5722..6873)
                     /locus_tag="PKN92_10980"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNZ49359.1"
                     /translation="MKILIAPDSFKECLDVFSVTDAIARGLLSGGASSEDIIRRPLAD
                     GGEGLLQVLVHAAGGSIHEKSVSGPLGEKVRASFALLSDGKTAVIEMAQAAGIHLIDP
                     EKRNPLFTTTYGVGELMRSALDAGARRLIVGLGGSATNDGGAGMAQALGVSLKNHEGE
                     ELSWGGAALASLAVIDCSRLDPRLKSTEVIGACDVTSPLCGARGASVVYGPQKGADAE
                     QVAVLDAALRHYGFLLEAACPPFHPMLPGAGAAGGLGAGLAAFAGAALRSGIDLVLSA
                     YGDFEEHCADAAAIISGEGRVDGQSTQGKVLSGVAGVARRHGVPLFVLAGKISGDLQT
                     LYDAGVTAVFPIAPFPMEEKEALRTAAAHLEQTARNLMRTVAALKRKKQ"
     gene            complement(7063..>7773)
                     /locus_tag="PKN92_10985"
     CDS             complement(7063..>7773)
                     /locus_tag="PKN92_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49360.1"
                     /translation="EGEAVEGEPVEGEAVEGEPVEGEAVEGEPVEGEMPVEGEPVEGE
                     PVEGEVVEGEVVEGEVEPVEGEVEGEVEGEPVEGEAVEGEAVEGEPVEGEAVEGEPVE
                     GEAVEGEAVEGEPAEGEAVEGEPAEGEAAEGEPVEGEPVEGEPVEGEPVEGEVEPVEG
                     EAVEGEVEPVEGEAVEGEALEGEKDDDTSGGCFGCRSGGKANKNLLLDDVKKVLADLL
                     LISLSALVLSAYAYTKRN"
BASE COUNT         1799 a         2250 c         1868 g         1856 t
ORIGIN      
        1 accggtcacg gttgtgtccg ccattgccgg agctgctttt ctgcaggctg ctcacattac
       61 ccgggaagaa cactggctga acacagcccg taatatatcc gtcttttttc aaaaagatct
      121 gccccgtcta ccgggagaag actccttctt ctgctttgcc tatgcgcccg gtgacacacg
      181 gcgcatccat aacgccaatc tgcttgccat agagcacatt ctgcacaccg cacgcctttc
      241 ggaagaaata gggacagcgg aagctcttct gcctgccctt catttttccc tcgcagcaca
      301 gcgggaagac ggcgcctggc cttacggagt ccgggagccc ggtgacccct ttgaaccccg
      361 gctcatgcgg catgtggaca atcatcatac gggttttgtt ttacggtcgc tgcacggcat
      421 cgattgcgcg gtacccggtc ttgtgccgga gcttgatgcc gtcatccgtc ggggctaccg
      481 attttacagg actctcttca gcaccgtcgg ccgcccgctc gaggaaggaa gacgctggcc
      541 ggtagatatt cacgctgcag cggaaggcat tttgtgtccc tctgttctag ccgagcgctt
      601 ccgtggcact gcgaacgatg ctctggctgt ccttcgctgg acacatgagc acatgcggga
      661 tgcggcaacc agtgccccct ggtaccggct ctatccgttt ttcgcctccc gaatcctgta
      721 cccacgctgg agccttgcct ggatgttcag agcactcgcc gaatacctct atgcaacaca
      781 ggacaacggt ccctccatac agcagcacta caatctttca cgctcttcca aagtctgata
      841 cagccgcggg cgccgcaggg agaagtcttc cagactctcg cgcatagact caagaatccc
      901 attcacggga agcccgccct gcagggctgt gcgcagatac ggcccacctg ccagcacatc
      961 aaagaaaggt cgccattgca gctcaggatg ccgcttatga attgcgctga gcactgccgt
     1021 caccatggca aaaggccgga tagtgcgtcg atccgtaatt tcgaattcaa taccgtggca
     1081 aagttgatcc ttgtattccg gattcgccgc cttacccgga atggactttg gaatataaag
     1141 agccggcttc aatgtgcagc cttccagtgc cgacggctcc accgccgcaa tcacctcttc
     1201 ggccttcagc cagggcgcgc cacagattct gaacggtctt tcggtacctc tgccttcatt
     1261 catattcacg ccttcaaaaa ggcaggtgcc aatataaagc agcgccgtat caggagtcgg
     1321 gatattgggc gaaggtggtg tccagggaag tgcgctttca ggatgggtga aatcatacca
     1381 ccagttttcc gccttaacaa tgcgaagttc cagttttttc cccttaaaaa attgctcctg
     1441 aaaaaagagt gccacctcac ccagggtcat gccatgccgt acaggaacag gtgcacaaca
     1501 cacagccgta ccaaattctt cagcaggctt tccttcaaga acaagaccgc ccagcggatt
     1561 gggtctgtcc agcacgagca caggaatctg cgcttccgca cagctcgcca tgcattcctt
     1621 catggtcgcc atataagtgt agtaccgtgc gccaatatcc ggcagatcca ccacaaacaa
     1681 atcgatcccc tgcaggtctt tccgatcagg agcactttta cctccataga gactgatgac
     1741 aggaacagtc ccgtcctttt tcgagccgac agcctcgccc gcttccgctt cgccctgaaa
     1801 gccgtgctca gggctgaaaa agcgcatcac ggtcgccgcc ccggactgcc cgagcaccag
     1861 cgcggcagaa cggcctgtct gatcctgagc ggcgctgttg gttaaaacgc ccagccgttt
     1921 tcctttgatc tcttcgaaat cattgcgaac aagggcatca aggcccgtat gcgcattcgc
     1981 actgttttta taaaaaagag aagcgacttt tgaataaaac gtacggcgca gcgcaccatt
     2041 gtttctgtgg gacacatcag gatgacaggt attgctcagg agcaccgcat atactcctgt
     2101 gcttctgtcc atccacatac tcgtacctgt ccagcctgta tgcccgaagg ccgttcggga
     2161 cggaagatat ccattggcac cactggtcca gggatcaatc caccatccca gaccctgcca
     2221 gggataaccg ggcacctgac cctcccgtgt gatatctgta agcgttgctt cagacagaat
     2281 ttttccggag agcagcgcct gacaaaacaa ttccagatcg gccgccgtac tgaacagccc
     2341 ggcatgcccg gaaacaccgc ccatagcaaa ggcgttctga tcatgcactt ctccgcggat
     2401 cagccgcttt ctccaggaac acttttccgt aggagcgcaa cgttctttga aagacggtgc
     2461 cggtttaaaa aacgtgttca ccattttcag cggttgaaaa atctgttcac gacaaaacac
     2521 atcaaaagac tcatgagtga tttcttcaat gacacgggtc agcaggataa agccgagatc
     2581 agaatatacc cgggtgcccc gctgatcccg ggatgccgcc agacgggcga tagcgtccgt
     2641 gtaagcgagc ggtccgctga gctctgtgta aaatgctttc cagctcacca gcccggccgt
     2701 atgcgtcagg acctggcgga tcgacacttt atccagattt gccagaggaa gataacggta
     2761 gagagcatca tccagatcca gctgggcttg ttcacgcaac aggagcacac tggtcgtagt
     2821 ggcaatcact tttgtcaatg atgccagatc ataaatcgtt tccgtgtcgg cacgttcttt
     2881 ttcaggaacg agcgcacgga agccggttgc ccctttaaaa atagtccggc cgttttcacc
     2941 cacacaggca acggcaccgg gggcctgtgc atcggcaaca gcgcgcgaaa gagcggacat
     3001 caaataatcg tgtggctgca tgctggattt ccattttaaa aacaggtctg aaattgctgc
     3061 gattataagc cggtcggatc gttaaaaaaa aatagcgcct cttccgattc ttttttaaag
     3121 gatgagaagg ggcgcttata acaatcaggt tcgacgccgt gcttttggca ctgcatctac
     3181 atggctattt tttcgccggc gcttttttca cgactgcttt cttggctgcg ggcttcgctg
     3241 ccggtttcgc ggctgctttc ttgactgccg gttttgctgc cggtttcgcg gctgctttct
     3301 tgactaccgg ttttgctgcc ggttttgctg ccggtttcgc ggctgctttc ttgactgccg
     3361 gttttgctgc aggctttgcg gctgctttct tgactgcagg tttcgcggct ggcttcgccg
     3421 cagctttttc tgcaactttt gccgcaggct ttgcagccgc tttcttcacg gccgctttct
     3481 tggcaggggc ttttttctca gaggctttct tttcgaccat ttggcttctc cttcttgtta
     3541 agccttagtg aacagtatgc cggctgtaca accacatggt gcaagccggg attgcgaccc
     3601 atgcacatat gaaatcgcaa cctgtaacag atcacactct gttaacgccc aattcaaata
     3661 atcagcagaa agctatctac tgattgccct tattatcatc atattttatg tcctatttca
     3721 aatccataag tttcttattt atcatggaaa tagttactga ctaaaaaccg accattgcgc
     3781 atttttaaaa gaaccttttt cggaatctcg tcttcttctc catatttatt tgtttagttg
     3841 taccaaactt ggagcagaaa aaacaacttt tattcctctg ctcaaaagaa aactggcaag
     3901 cagactatga atcccgtgaa atagattatt ctcgtgaatt ttattctttc tgtgttttat
     3961 gaaaagataa aagacaggtg ttcacaagag tctgctttta actctcaagg tttggcagcc
     4021 atcgaatctg ataagcccgg gagccagcct cgccaggacg gcccaggagg gtcagaacgg
     4081 tgccggattg cattactccg aatttcggaa aataggaaca catctccaat tgtgaagatt
     4141 ctatatattc tatatttcgg atcgttagca aaacacgcac tttgtcacga ataatcagca
     4201 gctcacggtc gggctcgtgc cagtgcactg tgcaggcagg atgaaaatga atatgcgata
     4261 tcggcgaacc gtcaaggcag tccgggctca aatagtcgca tatttcccag catacggatt
     4321 gggcatcaaa ttctattcga cggtgatata ccccgccctg atagtacttt accgctcccg
     4381 cgaagcggca gcctttttcc gttttttgta atgatgcagg aagtgcctca gcacgccgtg
     4441 ccatacgaaa gttcgaccaa tgttccgcct gctctctgcc ttcgggagtc agaacattgt
     4501 gcgccgaagt gctcctgcaa tgggcacggt aaggactctc ggcgtagccg tgcgttccgg
     4561 tgtccacgat aatgcgctta ccgctcaggc ttaactcgta ggagagcata tccccgtgac
     4621 tgtgcgcagc ctgatgagca ggagaaggcg ccgctgtttt tacaatcatg acagacccgg
     4681 cacaaaagtc accgagcaga aaataaccgg aagccgggca ggcgagactg cagtcggtac
     4741 gctgtatatc gacagtgcag gcagtgtccg ttaaaacgtc cgcacaggca cacaacacct
     4801 gaggaagggg agactcattc agcgaggcat ctccgaagag cggtatgtcg ccgtcaggat
     4861 ggcagagcac ccttgtaaag gaagccattt tcctgatggc ggaaacaaga aaagaaggtg
     4921 cttcttgcag caggctcaga acgatcagac tgtcccacag aagttcatta tgatagagaa
     4981 gacttcgctc aacatgcccg ccgtccggaa ggatttgctt ttccaactct gccttcagca
     5041 gcgccgttcc gtaccggcag atacggctgt cttccagcag actcccggca agcgtcagag
     5101 ccagggcatt tttcagcaga tgatttccct gcagatcatg ttccgctgta tggtaaagcc
     5161 aacgtgcctg ctgtgcgtag gattgaatga tatccttatc tttgatgtga taaacagcca
     5221 ggaggaccgg ccaggaaatc agacgctctg aaaggggcca gccgtcccat cctactcccc
     5281 tgccgggcgg attgtgtctt atccagttgt gcatccagac aagtacccgg tcacggtctc
     5341 ccagatgcgg cgtcagcagc ggagccatgg aaaattcccg cgctccccga aaaccgtgaa
     5401 ggtgatacag ccagagtttc ggaaggtcct gccgctgcca gagaagcact tcgtcctctt
     5461 gaaaagtacg gttcaaataa tgaaactgaa atttacggag ggcatccagt gaaatgcgtt
     5521 ctgtcgcccc tttttcggca gcagtggaaa gcagcgtccg caatgtatcc ggatacacag
     5581 ggtcgaacca tgctttccgg aagggacata aaagcgaaaa tcgtgcacgg ccgcgacgca
     5641 gaggacgaaa cagacgaaag acaatctgtc tccaggatag gaaatgaagg ctgtgccagt
     5701 aaagccgcca gtcgacagta gtcactgttt tttcctcttc agtgcagcca cggtacgcat
     5761 caggtttcgg gcagtctgtt ccaaatgagc ggcagctgtg cgcagcgctt ctttttcttc
     5821 catggggaag ggcgcaatgg gaaagacggc tgtgactcct gcatcgtaca gtgtctgcaa
     5881 atcaccgctt atttttccgg caagtacaaa caaaggaaca ccatgccggc gggcaacgcc
     5941 tgcaacaccg gatagcactt tgccctgagt ggactgccca tctacacgcc cttcaccgga
     6001 aataatagca gctgcatcag cacagtgttc ttcaaaatct ccataggcgg ataatacaag
     6061 gtctatccct gaacgaagtg ccgcaccggc aaacgcggca agaccggcgc caagtcctcc
     6121 cgccgctccg gcaccgggaa gcatcgggtg aaaaggcgga caggccgctt ccagcagaaa
     6181 cccgtaatgc ctgagcgctg catccaacac agcaacctgt tcggcatcag cacctttctg
     6241 agggccgtat acgacggaag cccctcttgc accgcacaac gggctcgtca catcgcaggc
     6301 acctatgacc tccgtgctct tcaggcgcgg atcaagccgg gaacagtcga taacagccag
     6361 agaagccagg gcagcgccgc cccaagaaag ctcctctccc tcatgatttt ttaaggacac
     6421 accgagcgcc tgtgccattc ctgcaccgcc gtcattggtg gcactgccgc cgagaccgac
     6481 tataagcctg cgtgcaccgg cgtccagggc gctccgcatt aattcaccga caccgtatgt
     6541 tgtagtaaaa agaggatttc ttttttccgg atcgatcagg tgaattccgg cagcctgtgc
     6601 catttcgatc actgcagttt ttccgtctga cagcaaagcg aaagatgctc tgactttttc
     6661 gccgagcggt ccgctaacac tcttttcatg aatagatcca ccagcggcat gtaccagcac
     6721 ctgcagcaat ccttcgccgc catcagctag aggacgacga attatatctt cagaagaagc
     6781 accgccagac agcagccccc gggctatggc gtctgtcacg ctgaaaacat ccaggcactc
     6841 tttaaaggaa tcaggtgcga tcagaatctt caaaagaact gcactccatc tcttatggtt
     6901 tacaacacgt ccttattatt cggcaagatc tcctgtccgg tcaagatttt acaggcgccc
     6961 actcctaatt ttttcagatg cccgaaaaaa gatccgctcc gtttccccag gaagggaagt
     7021 cacggagcgg acccgttttt tcacaaaaca gcaggcagtt aactagtttc tcttcgtata
     7081 ggcgtaggca ctcaaaacaa gcgcactcag agaaatgagc agcaaatcag ccagcacttt
     7141 cttcacgtca tcaagaagaa gattcttgtt tgccttgccg cctgatcggc atccaaagca
     7201 tccgcctgaa gtgtcgtcat ctttttctcc ttcaagcgct tcgccttcga cagcttcacc
     7261 ttctacaggt tccacttcgc cttcgacagc ttcaccttct acaggttcca cttcgccttc
     7321 gacgggttcg ccttcgacag gctctccttc tacaggctcg ccttctacag gctcgccttc
     7381 tgcagcttcg ccttctgcag gttcgccttc tacagcttcg ccttctgcag gttcgccttc
     7441 tacagcttcg ccttcaacag cttcgccttc gacgggttca ccttcaacag cttcgccttc
     7501 gacgggttca ccttcaacag cttcgccttc gacagcttcg ccttcaacag gttcgccttc
     7561 gacttcacct tcgacttcac cttctacagg ttctacttca ccttcgacga cttcaccttc
     7621 tacgacttca ccttcgacgg gttcgccttc tactggttca ccttctacag gcatctcacc
     7681 ttctactggt tcaccttcaa cagcttcgcc ttctactggt tcaccttcaa cagcttcgcc
     7741 ttctactggt tcaccttcaa cagcttcgcc ttc
//
LOCUS       DAOPWB010000139         7746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_9415_length_7746_cov_4.542322, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000139 DAOPWB010000000
VERSION     DAOPWB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9415_length_7746_cov_4.542322"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..163)
                     /locus_tag="PKN92_10990"
     tRNA            complement(88..163)
                     /locus_tag="PKN92_10990"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(128..130),aa:Asn,seq:gtt)
     gene            509..1381
                     /locus_tag="PKN92_10995"
     CDS             509..1381
                     /locus_tag="PKN92_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49361.1"
                     /translation="MKTHFPLWCIALTALVLAVSSSAPAQEETPDYFSCPESTVIGNP
                     ATPFDAEGYVPKYYPCEMSSVNEDIPFVAQIIELGQAEKICGVSFVGVEVDGDGNDCV
                     LEDATFTVILLVVDNEKDDEPEGEDQLKFALFKTAAAEKQTIGTLTVDGKEVTAYRYV
                     INDFGCQGVEAGTYAITIQHDPPANTKAEGCSFAWLNTLQGSDSLLDSTGMQLEDNVS
                     FCLNRGFEKHPADTNKDARISENEAREALERWQEGDDYMTFSIRAQYISQKGDGAYTL
                     NEDKEPPQSWEPAE"
     gene            complement(1836..3002)
                     /locus_tag="PKN92_11000"
     CDS             complement(1836..3002)
                     /locus_tag="PKN92_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNZ49362.1"
                     /translation="MPKETMTSRERVLKALNHQVPDRVPIDLGGNQTGIHKFAYEALL
                     AHLGLSENVEIMDAVQQLARPSETVLEHFHVDTRYIAAGAASGFDGKITTEMRDGMLW
                     HDLVDEFGVRWSMPEAHPMYMDISYSPLAHASLEDVREYPFPRGDDPGRFEGLRERAL
                     ALRRDTPYAVVSGISGVIYEICWYMRGLEAWFMDMMTAPEFCEALLDQTCKFWMDWFR
                     VFLDEVGDLVDVIMIGDDLAGQTGPLFNPDIYRSLVKPRHKKLVQYIRSRTKAKIWYH
                     TCGACLPFIPDLLDNGIDILNPVQISAANMEPARLKETFGDVLSFWGGGINAQHELPR
                     VSPDAVREAVRKNMEIFKPGGGYIFNSVHNIQAGVPGENIAALYDAAYRHGFYE"
     gene            3174..3899
                     /locus_tag="PKN92_11005"
     CDS             3174..3899
                     /locus_tag="PKN92_11005"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HNZ49363.1"
                     /translation="MRFLDISYAMPSKNLALDEALLETAASGKCCATLRLWESPVPFV
                     VVGSSQRVEEVVDLENCQSYAMPVLRRCSAGGAVLQGPGCLNYALALSYDAVPEVSSL
                     HASYEYILGKVSDALATLHVSTVREGVSDLAVQGMKCSGNSQRRKKNACLHHGTLLYD
                     MNADDMEDYLIEPEDRPAYRGTRNHAAFVQPLGFHVEQLRAALIRAFCPGAPAVQPFP
                     EEEEAMLLLARNKYSQAEWTFRR"
     gene            complement(3912..7118)
                     /locus_tag="PKN92_11010"
     CDS             complement(3912..7118)
                     /locus_tag="PKN92_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNZ49364.1"
                     /translation="MPLQCSVPGCLFLLLSALLAQPVVQAQVETGDVWRDATLYRDEW
                     GVPHVYANNPFAMAFVFGYAQAEDHAENMLLAYRLARGTLAAVLGEPWADSDAFSLKM
                     GHVRIAIEAWPTLDPMTVTLCEGFSMGVNAWLADHQHEAPSWADGVRPEDVLALWHAF
                     LMSQAPFDLPDLFRREPALPSANAWASAPERSDVGAAVLVMNPHQQYDGFFQWYEAHL
                     VLGGMNIYGATLRGLPVILQGHNESLGWALAPNMPDFADMYREDGGVSAPVRNPKDPR
                     AQVPDVSGETALLLEYMSQSSTYLVRTGSGMESRVVPSYIGERGPLFEDPARGLHSWL
                     IGGYRELNGFRQLLDMAFATELGAFQSALNGMQIPCFHIVYADRHGDIFYIYNTRTGT
                     RINEATLTPGGQGAPEPIRWYEPQTFKYAPVAWHRVIPPANLPYLVNPPSGYVQACGN
                     PPWTATEGAALNPGQWPVWLSADPDTYRARRIRQLLHAGIRSFRDHQSILYDVVASAA
                     FDTVPALLRSVEKRTDLTAQMHPDFHEGITLLRQWNCIAETGSPGMTFYHLWWTFTKT
                     RALAHYPSEQAVYQAIVSNDPAAEELMIRAVEDAARTLRNEYGTLQKNWGELHRIRRG
                     KREEPLPGALSGEPLFVASDTHFEPGRWISRYGYGFAMAVEFGEELRAVSLLPFGTSE
                     NPASPHYDDQLDMLLQRRFKKTHFHHDDILRNADQAIGKSITLLPLGVPGAMTLHSST
                     IMAARLNSAVEVPESLPPGLAPFSLYMQAERKPEGVPVALEITQMIPGALCDAEGFAQ
                     LALFRYEPGLGWQAMDWQTADPSRRLLYGRDSALAQWYVVLGPDGLVKSDDKAAAPAP
                     LMPEKYPVALDTLLDGHQTQPAIGGDGALFVPAPALPPVMSAEPPQETAGAENTAETV
                     PEAAPETMPGRTFKLEKLEGRDADSGGPKALRGIPGFHFGPRSGKGKAADKEAETASD
                     VGAAEGTKDRVPVEKGDVRQEEKTAQPSGQGTAIEAPAPVPKKEEESAARPERKRPPL
                     PDVIPQDPNFLFGPAAGEKKSGSGQPSPKGTFKLERLD"
     gene            complement(7280..>7746)
                     /locus_tag="PKN92_11015"
     CDS             complement(7280..>7746)
                     /locus_tag="PKN92_11015"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNZ49365.1"
                     /translation="GAVQYEPRYHERYIRIAADYKLPCRLPGKEMLAPFGAEWLLDLA
                     AELNVLGPENLYLDEPPSLEATESYYKERLTNLPQGKVSEMYIHCAVDEPEIHATTGS
                     ARRRVADTDFFSDPGTLQWIREQGIELISYRELRHLQREGKAMPRVERYGWD"
BASE COUNT         1745 a         2249 c         2029 g         1723 t
ORIGIN      
        1 acacgccgga aacgacgaga ccccgagggg atcaccccac ggggtcttgc gttaactaag
       61 caccgctgaa atcagcggat tgaattttgg ctccgcgagc aggactcgaa cctgcgacct
      121 agtggttaac agccacccgc tctaccgact gagctatcgc ggaatttgga gtgggttaag
      181 tgtatcaatt ttttgcagga gaatgcaaat ttcattaatc cgctattttt aaaaatgacc
      241 taccccaggc gccatactgt tttgtgtatg taacgtctcc tttcgcccgt tggtagaatt
      301 atcagccgga cagcttgcgg tcgggtgcgt ctctctccgc ccgcctgcgg ttgacagaat
      361 ttgttgatat gtggtagtct gtactaatat tgaaagaaat ttcatcgaaa tttccgatca
      421 ggcgcgtttt taggtggcac ggatattttc agggttgtct gaaaccttct ctgtgccaaa
      481 aacttgtcac ggttaaagga aaagaattat gaaaacgcat ttcccgctct ggtgtattgc
      541 gctgaccgcc cttgttctcg ctgtgtcctc aagtgcaccc gcccaagaag aaacgccgga
      601 ctatttcagc tgcccggaaa gcaccgttat cggaaacccg gcaactccct ttgatgccga
      661 aggctatgtc cccaagtatt acccctgcga gatgtccagt gtaaacgaag acattccctt
      721 cgttgcacag atcattgaac tgggtcaggc agagaagata tgcggtgtct cctttgtcgg
      781 cgtggaagtc gacggggacg gcaacgactg tgtcttagaa gacgctacct tcacagtcat
      841 cctgcttgtt gttgataacg aaaaagacga tgaacccgag ggcgaggatc aattaaaatt
      901 tgcgctcttc aaaaccgcag ccgctgaaaa acagaccata ggcacactta cggttgacgg
      961 taaagaagtg accgcctaca ggtatgtcat taacgacttc ggctgccaag gtgtggaagc
     1021 cggtacctat gccattacca ttcagcatga ccccccagcc aataccaaag cagagggatg
     1081 ctccttcgcc tggctgaata cacttcaggg cagcgactct ctgcttgact ccaccggcat
     1141 gcagctggaa gataacgtca gtttctgcct gaatcgtggc tttgaaaaac accctgccga
     1201 tacaaataaa gacgcccgga tcagcgaaaa tgaagcccgc gaagctctgg aacgctggca
     1261 ggaaggcgac gattacatga ccttcagcat tcgcgcccag tacatttccc aaaaaggcga
     1321 tggtgcctat accctcaatg aagacaaaga gccgccccaa tcctgggagc ctgccgaata
     1381 agggtcaacg ggtcatacag ccccgcacct tccgaagatt caggaacacc ctgcagcacc
     1441 cgttgcaggg tgttcttctt ttccgcaaca tccaatacaa catattgcgt gatccaaaag
     1501 agcacattgg cactaaaaac acaatctatt gtgtgcatct tgtcacttaa aacagattga
     1561 cgctgtatgt tgattttttt attgcaggaa aactcttgac atgcgcaata tattgtggta
     1621 tactactgat ggaatttgaa accgacgcac tgacctccag gcactctctt gcgcctttct
     1681 cagccctggg cgcaagtctg atcctgaaac cgtgctcctc ctgaacaacg cctggcaagg
     1741 cagcggttac aattccgccc taccgttttg ctggcggcgg gtagcccaag acccgccgcc
     1801 gcccctcaaa aaccttctgt cgttccccgt ggctcttatt cgtaaaaccc gtggcggtat
     1861 gcggcatcat acagtgcagc aatattttcg cctggtacac ctgcctggat attgtggacg
     1921 ctgttaaaga tatagccgcc gccgggcttg aaaatttcca tattttttct tacagcctcc
     1981 cgcacggcat ccggcgatac ccgcgggagt tcgtgctggg cattgatacc gcctccccag
     2041 aaactgagca catcaccaaa ggtctctttt aaccgggcag gctccatgtt ggctgcactg
     2101 atctgcaccg gattcagaat atcgatgcca ttgtccagca ggtccggaat aaaaggaagg
     2161 catgcgccgc aggtgtgata ccagattttc gcttttgtcc tgctgcggat atactgcacc
     2221 agttttttat gacggggctt cacgagactc cggtaaatat ccggattgaa gaggggcccc
     2281 gtctgtcccg ccagatcatc gccgatcatg atgacatcca cgaggtctcc gacttcatcc
     2341 agaaaaaccc ggaaccagtc catccagaat ttacaggtct gatccagcaa ggcttcacag
     2401 aactccggcg cggtcatcat atccatgaac caggcttcca atccgcgcat ataccagcaa
     2461 atctcataga taacgccgga aataccgctg accactgcat aaggcgtgtc ccttctgaga
     2521 gcaagtgccc gctcgcgcag cccttcaaag cgacccggat catcgcctct tggaaaggga
     2581 tactccctga cgtcttccag cgacgcatgt gccagcggcg aataactgat gtccatgtac
     2641 atgggatgcg cttccggcat agaccagcgc acgccgaatt catccaccag atcatgccac
     2701 agcattccgt cgcgcatttc tgtcgtaatt tttccgtcga aaccggaagc ggcaccggcg
     2761 gcaatatagc gtgtgtccac atgaaagtgt tccagcaccg tttctgaggg gcgtgcgagc
     2821 tgctgcaccg catccatgat ctccacattt tccgacaggc ccagatgagc aagcagtgcc
     2881 tcataggcga atttatgaat ccctgtctgg ttgccgccca gatcgatggg gacacgatcc
     2941 ggcacctggt gattcaaggc ttttaacaca cgttcccggg atgtcatggt ttctttcggc
     3001 atgggataat cctttttgtg aaagatgcgg gacaacacag ccacggcggt cccgtatcat
     3061 ttaaacagaa accatgaagc attgcaagac cgcttcgttt ttcccggcgc attcccaacg
     3121 ggcacgtata aatgatatac tttgcctcga aaaaaatata aggatctgta ctcatgcgct
     3181 tcctggatat cagctatgcc atgccctcaa agaatcttgc attggacgaa gcccttcttg
     3241 aaacagcggc atcgggaaaa tgctgtgcga ccctccggct ctgggaaagc cctgttccct
     3301 ttgttgttgt cgggtcgagc cagcgcgtgg aggaagtcgt cgaccttgaa aattgccagt
     3361 cttatgcgat gcctgtcctg cgtcgctgct ctgccggagg tgcggtgctt caggggccgg
     3421 gctgcctcaa ttacgcactt gccctttcct atgacgcagt gcctgaagtg tcttcacttc
     3481 atgcctctta tgaatatatt ctgggcaaag tatcggatgc cctcgcaaca ctgcatgtat
     3541 cgacggtgcg ggaaggtgta tcggatttgg cggtgcaggg catgaaatgc tcgggcaatt
     3601 cacaaaggcg gaaaaaaaat gcctgcctcc accatggcac gctgctctat gatatgaatg
     3661 cggacgacat ggaagattat ctgatcgagc ccgaagaccg cccggcatac cgcggaacaa
     3721 gaaatcacgc cgcctttgtg cagcctctgg gatttcatgt ggagcagctg cgggctgccc
     3781 tgatccgcgc cttctgcccg ggtgcccctg cggtacagcc ttttcctgaa gaagaggagg
     3841 cgatgctcct tcttgcccgc aacaaatatt cacaggcaga gtggacgttc cggcgttagc
     3901 cggcagatta atcagtccaa tcgctccagt ttgaaggtgc ctttcggaga aggctgtcct
     3961 gagccggatt tcttttcacc tgccgccggt ccgaaaagga aattgggatc ctgcggaatg
     4021 acgtcgggca gcggaggacg ctttctttcc ggtctggcag ccgattcctc ctcttttttc
     4081 ggaacaggtg ccggtgcttc tattgctgtc ccctgcccgg acggctgcgc tgttttttct
     4141 tcctgccgca catcgccctt ctccaccgga acgcgatctt tcgttccctc cgccgccccg
     4201 acatcagagg cagtttccgc ctccttgtct gctgcttttc ctttgcctga tctggggccg
     4261 aaatgaaacc ccggaatgcc gcgaagtgct ttcggccccc cggaatccgc gtccctgcct
     4321 tccagtttct ccaatttgaa cgtgcgcccc ggcattgttt ccggcgccgc ttccggaacg
     4381 gtctccgccg tattttctgc gcccgctgtt tcctgcggag gctctgcaga catcacaggc
     4441 ggcaaagccg gcgccggcac aaaaagagcg ccgtctccac cgatggcggg ctgcgtctga
     4501 tgtccgtcca gcagtgtatc caatgcaacc gggtatttct ccggcatgag tggcgcaggc
     4561 gcagcagcct tatcgtctga tttcacaaga ccatccggac caagcaccac gtaccattgc
     4621 gccagtgcgc tgtcccgtcc gtacaggagt cgccgggaag gatcggctgt ctgccagtcc
     4681 atcgcctgcc agcccagccc cggctcatag cggaaaagtg caagctgcgc aaatccttcg
     4741 gcatcgcaga gcgccccggg gatcatctgg gtgatctcca gcgccacggg caccccctcg
     4801 ggctttcgct ccgcctgcat gtacaggctg aaaggggcaa gcccgggcgg cagcgattcc
     4861 ggcacttcaa ctgcgctgtt aagccgtgcc gccatgatag tgctgctgtg gagcgtcatg
     4921 gcaccgggca ctccgagcgg cagcagcgta atactttttc caatcgcctg atccgcatta
     4981 cgcaggatat catcatgatg gaaatgcgtt tttttgaaac gccgctggag cagcatgtcc
     5041 agctgatcgt cataatgagg tgatgcggga ttttcggaag tgccgaaagg caggagactc
     5101 acagcgcgga gctcctctcc gaactcaact gccatggcaa atccgtagcc gtaccggctg
     5161 atccatcttc cgggttcaaa atgcgtgtcc gatgccacaa aaagaggctc tccactgaga
     5221 gcgcccggga gcggttcctc tctttttcct cttcggatcc tgtggagttc gccccagttc
     5281 ttctgaaggg ttccgtactc attgcgcagg gtacgtgccg catcttccac cgcccggatc
     5341 atcagttctt ccgctgcagg atcattgctg acaatcgcct gataaaccgc ctgctcggag
     5401 ggataatgcg cgagagcacg tgtttttgta aacgtccacc acaggtggta aaaggtcatg
     5461 cccggcgatc ctgtttccgc aatacagttc cactgccgga gaagagtgat gccttcgtga
     5521 aaatccggat gcatctgggc tgtcagatcg gtacgctttt ccacgcttct aaggagtgcc
     5581 ggcaccgtgt caaaagcggc ggaggcaacc acgtcataca aaatggactg atgatcccgg
     5641 aaactgcgta tgcctgcatg cagcagctgg cggatgcgcc gggcacggta ggtatcggga
     5701 tccgccgaaa gccagaccgg ccattgcccg gggttgagcg ctgccccttc cgttgcggtc
     5761 cagggcggat tgccgcatgc ctgcacatac ccggagggcg gattcaccag atagggcaga
     5821 tttgcgggcg gaatcacccg gtgccaggca acgggcgcat atttgaaagt ctgaggttca
     5881 taccagcgta taggctcggg cgcaccctgc ccccccggag tcaacgtcgc ttcattgatc
     5941 cgtgtgccgg tacgggtgtt gtaaatataa aatatatcgc cgtggcggtc tgcataaacg
     6001 atatgaaagc aggggatctg catcccgttc aatgcactct ggaatgcgcc cagttctgtt
     6061 gcgaaagcca tatccaggag ctggcggaat ccgttgagtt cccggtagcc gcctataagc
     6121 caggagtgca gaccgcgggc aggatcttca aacagaggcc ctctttcgcc gatgtaggaa
     6181 ggcaccaccc ggctctccat ccccgagccg gtccggacaa gataggtgga cgactgagac
     6241 atgtattcca gcagcagcgc cgtctctccg gaaacatccg gaacctgtgc ccgcggatct
     6301 ttagggttgc gcaccggtgc ggacacgccg ccgtcctccc ggtacatatc ggcgaaatca
     6361 ggcatattcg gtgccagcgc ccagccgagc gattcattat gcccctgcag aatgacagga
     6421 agcccgcgca gcgttgcacc gtatatattc atgccgccca gcaccagatg cgcctcatac
     6481 cattgaaaaa aaccatcgta ttgctgatgg ggattcatca ccagcacggc cgccccgaca
     6541 tcgctccgct cgggtgcgct tgcccaggca ttggctgaag gcaatgccgg ctcgcgcctg
     6601 aacaaatcgg ggaggtcaaa gggcgcctgg ctcatgagaa aagcatgcca caacgccagc
     6661 acatcctcgg ggcgcacgcc atccgcccag gaaggcgctt catgctggtg gtcggcaagc
     6721 caggcattga cgcccatgga aaagccctca cagagggtaa cagtcatggg atccagggtc
     6781 ggccacgctt caatcgcaat ccggacatgc cccattttca aagaaaaggc atctgaatcc
     6841 gcccagggct cccccagaac ggcagccagc gttccccggg caagccgata cgccagcagc
     6901 atgttctccg catgatcttc cgcctgtgcg tagccaaaaa caaaggccat cgcaaaggga
     6961 ttgttggcat atacatgggg aacgccccac tcatcccggt ataaggtggc atcacgccag
     7021 acatcgcctg tttcgacttg cgcctgaacg acgggctggg caagcaatgc cgacagcaaa
     7081 aggaaaagac accccggcac agagcactgc aggggcatga atgctttgct tttggatatt
     7141 ttcgggataa gcagagaagt cagcataccc aatagtatac cgcctgccac agcaggattg
     7201 catgaagcgt taccggcagg cttctgaaaa gaattcggag cttcagcact tcgccttaca
     7261 atcagaaatt cagcctgttt cagtcccacc cgtatcgctc aacacggggc atggcttttc
     7321 cttcgcgctg gaggtggcga agctcgcggt aactgatcag ttctatcccc tgctccctga
     7381 tccactgcag ggtgccggga tcgctgaaga aatccgtatc tgccacacga cgtcgtgccg
     7441 atcctgttgt ggcatggatt tcaggctcat ccacagcaca atgaatatac atttcgctga
     7501 ctttcccctg cggaagattt gtcagcctct ccttatagta actttccgtc gcttcaagag
     7561 aaggaggttc atccagatac agattttcgg gaccgagcac attcagctcc gcggctagat
     7621 caaggagcca ttccgcaccg aagggcgcca gcatctcttt gccgggcaga cggcagggca
     7681 gtttgtaatc ggcggcgata cgaatgtacc gctcgtgata tcggggttca tattgaaccg
     7741 caccca
//
LOCUS       DAOPWB010000140         7716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_9469_length_7716_cov_4.268503, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000140 DAOPWB010000000
VERSION     DAOPWB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9469_length_7716_cov_4.268503"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..428)
                     /locus_tag="PKN92_11020"
     CDS             complement(57..428)
                     /locus_tag="PKN92_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49366.1"
                     /translation="MMGYEWLHNESGLALVAALTAVVWAFFRGSAFLERIRTQKLERA
                     LEALEAAVEATYRDYVRLLKSTRESGQLTPGEEQEARRRAKEKGAALAQAAGINLAHT
                     LGAEYLDLWINRLVRKLKTGS"
     gene            complement(446..907)
                     /locus_tag="PKN92_11025"
     CDS             complement(446..907)
                     /locus_tag="PKN92_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49367.1"
                     /translation="MEIQQGRNDIYLTLSRDGKPPSHFKLREFENSGGLVMVHRSTLE
                     SLEAVRADLCAQYGEEVQVIITDGIRTQEDNERIAEKYGWADEGGAVARMSKHLTAYG
                     GIAVDITARVARTGARISQRTLGDICRRHFDFVKDDYRDGHVHADNRNRAR"
     gene            complement(921..1964)
                     /locus_tag="PKN92_11030"
     CDS             complement(921..1964)
                     /locus_tag="PKN92_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49368.1"
                     /translation="MNHNDTLLKEQLRFLRQDKDRLGALRGRELYETLSGLHLTHRQY
                     FRALDTSLDEYCREAFGTDLQAATVERFFRSDPDAKWLFPEVVREAVMAGIRTKPRYP
                     ELIVRDEPIQGAVYDMPYVDEDKENEEMRPVAEGAAIPESSIQYGDRVVRLDKKGRGV
                     IASYEVIRRMSVDLLRVHLRRLGERLGRSLDIRLTEVLAEGDNSGKTAPEVIESEEPG
                     KWQYGDLVNAYLYLSLANNFTPTHVLTGPGATAAILLMDHTANMLLFDFAKTGQYPTP
                     LGMKLVPMPDLPNNRIIVMDAGYAVQKLTEQDLLLENDKLINQQWDRTYLTVITEFAI
                     LYNKARVVISNGN"
     gene            complement(1994..2377)
                     /locus_tag="PKN92_11035"
     CDS             complement(1994..2377)
                     /locus_tag="PKN92_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49369.1"
                     /translation="MAFGDFGGPVTSLIITCRAADNNGTVAIKKGDALKLVDNYTVTN
                     VLADGDAVFGEALEDCAEAGAALAVRVGGICTFSFTGEGFGPDGFSGVVGSSEAGKVR
                     PGTERNARGLIVRTRNSIKSLEVLL"
     gene            complement(2423..3121)
                     /locus_tag="PKN92_11040"
     CDS             complement(2423..3121)
                     /locus_tag="PKN92_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49370.1"
                     /translation="MFWEIAEVSRCGTAPDTQEEQGRFVLHRHDDGEGVHLDLRLEWG
                     DTLSGWRIAGENLETGLWATEKMPHPADWLTQDRGLERKSAGLWRWEERSEDRRRVAL
                     QIGEETVRITLERRRGISAETVRALSDLAKESKMPFSALAGLAADGLQARAREIERFC
                     ALSRMLDGEGFDEAGWRSLFSGMSLREISDRLAHVEIRHDRLHPPLPVSLPEKLTEDE
                     GTSRMRHAYQILHS"
     gene            complement(3162..3365)
                     /locus_tag="PKN92_11045"
     CDS             complement(3162..3365)
                     /locus_tag="PKN92_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49371.1"
                     /translation="MEEKEGCVTRCSFERAAEVEFRALNAAVREVQERLGRLESTLMR
                     GAALLMANLAGMAATLAGQLLGK"
     gene            complement(3395..4093)
                     /locus_tag="PKN92_11050"
     CDS             complement(3395..4093)
                     /locus_tag="PKN92_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49372.1"
                     /translation="MELDAFVFSRRGTLAQERATGAQDEEDVMAAVNRFTLKPLKKEE
                     VALFTLDLCNNKIDRHFSRFPEEELEKVNRLTPGRPLMERHDLTGTLPRGTFFRSQLH
                     HDGGHISVRPEVYVLRIDDNRDFILNIEGGVYRETSISFSFRKPECSICGKDLRRCSH
                     IPGRQYGDALCHFIMRDVVDVVEGSVVSAGSQGTGFVSQERAIPMTASAALNRAREEF
                     HRPVSLWSAAWALE"
     gene            complement(4173..5432)
                     /locus_tag="PKN92_11055"
     CDS             complement(4173..5432)
                     /locus_tag="PKN92_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49373.1"
                     /translation="MKQRGWAAWWRRVKGSSNQPKRGSPPPGGLSAVSQGTDTKRFFP
                     DSFLPLPGRIPFESLHLYRQLRDSIPDISDAVWTWKRLCQTERKVLIEELGNGVSRDA
                     VLNRLTAFENRINMRGGGLAGLLDLFYSSLFTFGAAALEAVPGGACSGIHDVVPVDVW
                     TVRFRHNGQRLMPWQVTDGNEIALPEDFFFYVGLDRDGTNPYGRSMIAALPGLVRIQQ
                     ELLSDMARATRNAGWNRLHVRYHPEDRETEEEPEAYKERIRTNLHRLSDVLKSTDIDQ
                     NLITLDNVSVEVLAGNQRNQVFYENHKAVEEQVITGMHMMPVLLGRNYGSTETYGTAQ
                     FEVVNRQVQTVNRQVADMLTRIYSLELALSGINARVRVSLEGNRTVDVLRAAQTRRVE
                     IDTTLRLLEQGLISEREAKARLQRADS"
     gene            complement(5600..7432)
                     /locus_tag="PKN92_11060"
     CDS             complement(5600..7432)
                     /locus_tag="PKN92_11060"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ49374.1"
                     /translation="MTEGRRDFVCLILLCALCLVVYGRCATFEHTLYDDPNIIFENEH
                     VQQGLTLESIRWAFTTPNFGLYMALPTLTFLIDRDLFGDWDGGYHLSTLFWHLLCVAS
                     FYLVMRRLTGAFAPVLAASLLVAVHPVQAMTINWISARNEIIPPVFMLWSIDFYRQGT
                     LTGVSGFDRKRLRLYGASLLLMLLGLLSKQGIVLLPVVLLLLDYWPLQRITLQPDKPL
                     QTLNRMLRLSLEKIPWFLLSVIGVGLAFYGKMNFGAFEDETLQSPLNNIGFSAVSFVR
                     YLFHLVYPERYLMAYSAFQWTPAWWMVAGSVLLLGSITATAFYLLWRQPWMIVFWAWF
                     VCFLLPVSGLVRYASESIALRYLYAPGMGIYLLVAFGLFSLIQRFLKPPRPSGKTPSN
                     EKKSKAGKMGEVISQKEKAPPLFWSIVAVLTIACAAQAYRQSGFWRNAQSIAERSLVV
                     SGGTNALAHNHLSVLFERQGQQAKYFAHSKAAFDLEPGRSAWRANYATALGRRGQYRE
                     CLEITEPLLKTLPDSVVANNLYGLALTGLGRYEEALPYLEKAVSIEPRYVPAQYNLGA
                     CLEQLGRLEEALSHYEQALAEQPGHSLSQQRRAIVQKQLELQRK"
     gene            7480..>7716
                     /locus_tag="PKN92_11065"
     CDS             7480..>7716
                     /locus_tag="PKN92_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49375.1"
                     /translation="MLFRSKGTNSDSLLFLRAPAWERQSPHWQGRVWSLFLGGMSRGI
                     TPTQIQVPVFFLTRLYANAEIGVPREELHVSGAAS"
BASE COUNT         1620 a         2408 c         1927 g         1761 t
ORIGIN      
        1 aaaaagacgg caggacattt cgccctgccg tccggcattg tgcctttccc tcctgctcag
       61 ctccccgtct tcagtttgcg taccagtctg ttgatccaga gatcaagata ctcggcaccg
      121 agtgtgtgcg ccagattgat acctgccgcc tgtgccagtg ccgctccttt ttctttggcg
      181 cggcgtcgtg cttcctgctc ttcaccgggt gtgagctgcc cgctttcccg ggtgcttttc
      241 agcaggcgca cataatcccg gtaggttgct tccacagccg cttccagcgc ttccagcgca
      301 cgctccagtt tctgagtgcg gattctctcc agaaaagcgc ttcccctgaa aaatgcccat
      361 accaccgccg tcagggctgc aaccagggca agcccgcttt cattgtgtaa ccattcatag
      421 cccatcacag actgtctcct tctcttcagc gtgcccggtt ccggttgtcc gcatgaacat
      481 gaccatcccg gtaatcatct ttgacgaaat caaaatggcg tctgcagata tcgcccagtg
      541 tacgctgtga tatccgtgca cctgtccgcg ccacccgtgc ggttatatcc acggcgatgc
      601 cgccgtatgc cgtcagatgt ttcgacatgc gtgccacggc accgccttca tccgcccatc
      661 cgtatttctc ggcaatgcgc tcgttatctt cctgcgttct gattccgtcg gtgatgataa
      721 cctgaacttc ctcgccatac tgtgcgcaca gatcggcccg gactgcttcc agtgattcca
      781 gcgtactgcg gtgcaccatg acgagcccgc ccgaattttc aaactcacgc aatttgaaat
      841 ggctgggagg cttgccgtcc ctgctcagtg tcagatagat gtcattacgt ccctgctgta
      901 tttccataca tcgcctttcc tcagttgcca ttggaaatca cgacacgcgc cttgttgtac
      961 agaatcgcaa actccgtgat caccgtcaga taggtacgat cccactgctg gttgatcagc
     1021 ttgtcatttt ccagcaacag atcctgctcc gtcagtttct gtactgcata gcccgcatcc
     1081 atcacgatta tacggttgtt cggaaggtcg ggcatgggca ccagtttcat gcccagcggc
     1141 gtcggatact gccctgtttt cgcaaaatca aaaagcagca tgttggcggt atgatccatc
     1201 agcagaatgg ctgccgtcgc tcccggacct gtgagcacat gtgtcggggt aaaattattt
     1261 gccagcgaca ggtacagata ggcgttgacc agatctccgt actgccattt gcccggttcc
     1321 tctgattcga tgacttccgg agccgtcttc ccggagttgt ctccttccgc cagcacttcc
     1381 gtcagccgta tgtccaggct tcgtcccagc cgttcgccaa gccgtcgcag atgcacccgg
     1441 agcagatcca cggacatgcg gcggatcact tcataggaag caatcacgcc cctgcctttt
     1501 ttgtccagac gcaccacgcg atcgccgtac tgaatggagg attccggaat cgccgcccct
     1561 tccgcaacag ggcgcatctc ttcgttttcc ttatcttcgt ccacataagg catgtcatag
     1621 acggcgccct gaatcggctc atcccgcacg atcagttccg gataacgcgg cttggtgcgt
     1681 atacccgcca tcacggcttc ccgcactact tccggaaaaa gccacttggc atcgggatcg
     1741 ctccggaaaa aacgctccac cgtcgccgcc tgcagatcag taccgaaggc ctcgcggcaa
     1801 tattcgtcca atgatgtatc cagtgcccgg aaatactgcc tgtgcgtcag atgcagcccc
     1861 gacagtgttt cgtacagttc tctgccccgc agcgcaccca gccggtcttt atcctgtcgc
     1921 aggaaacgca gctgttcttt caataatgtg tcgttatgat tcatccatga tttcctttcc
     1981 cgctgtaacc tgattaaaga agcacttcaa gggatttgat gctgtttctg gtacgcacga
     2041 tgagtccccg ggcattgcgt tctgttccag gcctgacctt tcccgcctcc gacgagccga
     2101 caacgccgct gaaaccgtcg gggccgaagc cttcgcccgt gaaggaaaaa gtgcatatgc
     2161 cgccgacacg cacagccagc gccgcccctg cttcggcgca gtcttccagg gcctctccga
     2221 aaaccgcatc tccgtcagcc agtacattgg tcaccgtata gttatcaacc aatttcaggg
     2281 catccccttt ttttattgcc acggttccgt tgttgtccgc cgcccggcag gtaatgatca
     2341 gacttgtaac cgggcctcca aaatcaccaa atgccatatt gtttcctttc cttattactc
     2401 ttgttctgct gccgttcatg cattacgagt gaagaatctg ataggcgtgc cgcatccgtg
     2461 atgttccctc gtcctccgtc agtttttccg gaagcgatac ggggagcggg ggatgcaggc
     2521 ggtcatgcct gatttccaca tgcgcgagcc tgtcgcttat ttcacgaagg gacatgcccg
     2581 aaaaaagact gcgccagcct gcttcatcga aaccctcgcc gtccagcatt ctgctcagcg
     2641 cacagaaccg ctcgatctcc cgtgcccgcg cctgaagccc gtccgctgcc agtcctgcca
     2701 gtgcggaaaa aggcatcttt gattcttttg ccagatccga aagagcgcga accgtttcgg
     2761 cgctgatgcc gcggcgccgt tccagtgtaa tgcgtacggt ctcctctccg atctgcaagg
     2821 cgaccctacg cctgtcctca ctccgctcct cccagcgcca cagccctgca gacttccgtt
     2881 ccagcccccg gtcctgtgtc agccagtcag ccgggtgagg cattttttct gtagcccaga
     2941 gccctgtttc cagattttcg cctgcaatgc gccagcccga aagcgtgtcg ccccattcaa
     3001 ggcgcagatc aagatgcaca ccttcgccgt catcgtgccg gtgcagcaca aagcgcccct
     3061 gttcttcctg tgtatccggt gctgtgccgc atcggcttac ttccgcaatt tcccagaaca
     3121 tgcgttcttt tctcccttcc ttttcggttt cagacccttg cttatttccc gagcagctga
     3181 cctgccagcg tggcagccat tcctgccaga ttcgccatga gcagcgccgc accgcgcata
     3241 agcgttgatt ccagtctgcc cagccgctcc tgcacttcgc gcaccgcggc attaagcgca
     3301 cggaattcca cttccgccgc ccgctcaaaa gagcaccgcg tcacacagcc ctctttctct
     3361 tccatgcttc tgcacctcct tatgatccct ctgtttattc cagtgcccac gccgcactcc
     3421 acagcgaaac aggacgatga aattcctcac gtgcccggtt cagcgctgca gacgctgtca
     3481 tgggaatggc gcgttcctgt gagacaaagc ccgtgccctg tgatcctgcg gaaacgaccg
     3541 agccctctac cacatccacc acgtcacgca tgatgaagtg acacaacgca tccccgtact
     3601 gccttccggg tatgtggctg cagcgcctca gatctttgcc gcagattgaa cattcaggtt
     3661 tgcggaagga aaaggaaatg gaggtctccc gataaacgcc gccctcaatg ttcagaataa
     3721 aatcacggtt gtcatcgatg cgcagcacat agacctccgg gcgcaccgaa atgtgcccgc
     3781 cgtcatgatg cagctgcgac cggaaaaaag tgccccgcgg caatgtgccc gtcaaatcat
     3841 gccgttccat caggggccgt cccggcgtca gccgattgac cttttccaac tcctcttccg
     3901 ggaagcggct gaagtgccgg tcaattttgt tgttgcataa atccagggta aacagcgcca
     3961 cctcttcttt tttcaacggc ttcagtgtaa agcggttgac tgcggccatc acgtcttctt
     4021 catcctgtgc ccctgtcgca cgctcctgcg ccagtgtgcc ccgtctgctg aatacaaagg
     4081 catccaattc catgtctgcc gattcctcct tcctgtttct gtgccgatcc ctccgcccgc
     4141 acgggcggat ccgtgtgttg cccggaatag cctcagctgt cagccctttg cagccgtgcc
     4201 ttcgcttccc tttcgctgat cagcccctgc tcaagcagcc gaagggtggt gtcaatttcc
     4261 acacggcgtg tttgagctgc ccgcagcaca tcgacggtgc ggttcccttc cagcgaaaca
     4321 cgaacccggg catttatacc cgacaaggca agttccagcg aatatatccg ggtcagcatg
     4381 tccgccacct gtcggtttac ggtctgcacc tgccggttga ccacctcaaa ctgcgccgtg
     4441 ccgtaggtct ctgtggatcc gtaattgcgc cccagaagca ccggcatcat atgcattcct
     4501 gtgatgacct gctcttccac tgctttgtgg ttttcataaa acacctgatt ccgctgattt
     4561 cctgcaagca cttccaccga cacgttatcc agcgtaatca gattctgatc gatgtcggtg
     4621 cttttcaata cgtcactcag gcggtgcaga ttggtgcgaa tccgttcctt atacgcttcc
     4681 ggttcctcct cggtttcccg gtcttcgggg tgataccgca catgcagccg gttccatccc
     4741 gcattgcgtg tcgcccgtgc catgtccgac agcagctcct gctgaatgcg tacaagcccg
     4801 ggcagtgcgg caatcatgct tctgccgtag ggattcgtac cgtcacgatc gagacccaca
     4861 taaaagaaaa agtcttccgg aagggcgatt tcgttgccgt ctgttacctg ccacggcatg
     4921 agccgctgtc cgttatgccg gaaacgtacc gtccacacat ctaccggcac cacatcatga
     4981 atcccgctgc aggcaccgcc gggcaccgct tccagcgccg ccgccccgaa ggtgaaaaga
     5041 gacgagtaaa acaggtcaag cagtcccgca agcccgccgc cgcgcatatt gatcctgttt
     5101 tcgaaagcgg tcagtctgtt cagcacggca tcccggctga caccgttacc cagttcctca
     5161 atcagcactt tccgctctgt ctggcagagc cgcttccagg tccacaccgc atcggatata
     5221 tccggaatcg aatcacggag ctggcggtag agatggagcg actcaaaggg gatgcgccca
     5281 ggcaacggta aaaaactgtc gggaaaaaaa cgcttcgtat ccgtcccctg actcacggcc
     5341 gacagaccgc ccgggggagg ggagcccctt ttaggttgat ttgaactgcc tttgacgcgc
     5401 cgccaccatg ccgcccagcc tctctgcttc atctgtttcg cctcctgatt gtttcaccgt
     5461 accggagtat cccgcatcca cccattacag acggggcgtt gcaggattca ggagaggcac
     5521 aggcatttta tccaatgctg ccagcagtaa aaaagaggct gtgccggtgc ggacgctgtc
     5581 agcagggtaa acgctccgtt tacttgcgct gaagctcgag ctgcttctgt acgatcgccc
     5641 ggcgctgctg cgacagactg tgccccggct gttccgcaag cgcctgctcg taatgtgaca
     5701 gtgcttcctc caggcgaccc agctgttcaa gacatgcgcc aagattgtat tgggcgggaa
     5761 catagcgcgg ttcaatggaa acggcttttt cgagataagg cagggcttcc tcatagcggc
     5821 ccagtcccgt cagcgccagt ccgtacagat tattggccac cacggaatcg ggcagtgttt
     5881 tcagcagcgg ttccgtaatc tccagacatt ccctgtattg tccgcgcctg cccagtgctg
     5941 tcgcataatt ggcgcgccag gcgctgcggc ccggttcaag atcaaaggcg gctttactat
     6001 gggcaaagta ttttgcctgc tgtccctgtc tttcgaaaag aacagacaga tgattatgcg
     6061 ccagtgcatt ggttccgccg gataccacaa gcgaacgctc cgcaatactc tgggcgtttc
     6121 tccagaaacc gctctggcgg tatgcctgcg cagcacaggc gatggtaagc accgccacaa
     6181 tgctccagaa cagaggaggc gccttttctt tttgagaaat gacctcgccc attttgcccg
     6241 ctttgctttt cttttcgttc gacggcgttt tgcctgatgg gcgcggcggc ttcaaaaaac
     6301 gctgtatcag agaaaaaagt ccgaaagcga caagcaggta gattcccatg cccggcgcat
     6361 aaagatagcg cagtgcaatt gattccgaag cataacggac aagaccgctg accggaagaa
     6421 gaaagcagac gaaccacgcc cagaacacaa tcatccaggg ctgccgccag agcaaataga
     6481 aggcagtggc ggtaatgctg cccagcagca gcactgatcc cgccaccatc caccaggcag
     6541 gcgtccattg aaatgcggaa taggccatca gatagcgttc cggataaacc agatggaaaa
     6601 ggtagcgcac aaaagagacg gcggaaaaac cgatgttgtt caaaggagac tgcagcgttt
     6661 catcctcaaa agcaccgaaa ttcatcttcc cgtaaaaagc gagccctacg ccgattacgg
     6721 aaagaaggaa ccacggaatt ttttccaggc tcagccggag catccggttc agggtctgca
     6781 ggggtttgtc aggttgaagc gtgatgcgtt gcagcggcca gtaatccagc agaagcagca
     6841 ctacaggcag cagcacaatc ccctgtttgc tcaaaagtcc caaaagcatc agcagaaggg
     6901 aagcgccgta caggcgcagc cttttcctgt cgaaccccga tacgccggtc aaggtgccct
     6961 ggcggtaaaa atcaatgctc cacagcatga aaacaggcgg tatgatctca ttgcgtgcag
     7021 agatccagtt gatcgtcatt gcctgcaccg gatgcaccgc caccagcagt gatgccgcca
     7081 gcacgggcgc aaaagcgccg gtaagccgtc gcatcaccag ataaaaactt gccacacaca
     7141 ggagatgcca gaaaagcgtg ctcaggtggt acccgccgtc ccagtcgcca aacagatcac
     7201 ggtcgatcag aaaagtcaac gtgggcagcg ccatgtacag accgaaattc ggcgttgtaa
     7261 aagcccagcg gatgctctcc agcgtcaaac cttgctgcac atgctcattt tcaaaaatga
     7321 tattcggatc atcataaagg gtgtgttcaa aggtggcgca ccggccgtaa acgacaaggc
     7381 agagcgcaca taatagaatc aggcatacaa aatcacgacg accttcggtc atgtctggtg
     7441 tctccttcat aaacggaatg gctgtctctt ctggagtata tgctcttccg gtccaaaggt
     7501 acaaacagcg acagcctcct ttttcttcgt gcgccagcct gggaacgcca atctccgcat
     7561 tggcaggggc gtgtgtggtc tttgtttttg ggaggtatgt cccggggcat tactccgact
     7621 cagatccaag tgcctgtctt tttcctcact cgcttgtatg ccaatgcgga gattggcgtt
     7681 cccagggaag aattacatgt ctccggcgct gcttct
//
LOCUS       DAOPWB010000141         7704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_9489_length_7704_cov_5.075696, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000141 DAOPWB010000000
VERSION     DAOPWB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9489_length_7704_cov_5.075696"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..655)
                     /gene="ilvN"
                     /locus_tag="PKN92_11070"
     CDS             complement(137..655)
                     /gene="ilvN"
                     /locus_tag="PKN92_11070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNZ49376.1"
                     /translation="MTEKKHTICVLVANQFGVLARVSGMFSARGYNISSLCVGETDDP
                     TVSRMTVIVRGDDSVLAQIMQQLNKLVDVIKVEDLTAASYVERELIMIRVAATGTVRS
                     EVLGIVEIFRGKIVDLDAKCITVEVTGAQEKIQAFLEMMRGYGILEMVRTGEIAMARA
                     ANNSASQIRVQE"
     gene            complement(652..1242)
                     /gene="pth"
                     /locus_tag="PKN92_11075"
     CDS             complement(652..1242)
                     /gene="pth"
                     /locus_tag="PKN92_11075"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNZ49377.1"
                     /translation="MKLIAGLGNPGEQYRNTRHNLGFVVIDRLAALLNVEISATRDKA
                     HTVRFQHRGTSVMLVKPQTFMNLSGQAVAPLARRAGCLPEDILAVSDDRHLPLGVLRL
                     RAGGGTGGHNGLKSLVADLGSQEFHRLRLGIGNHAEAPDRLSNYVLGKFAPWEISDVE
                     KMVREAAEAARCWLEEGIETAMNRYNRRNDAPGEKS"
     gene            complement(1287..1907)
                     /locus_tag="PKN92_11080"
     CDS             complement(1287..1907)
                     /locus_tag="PKN92_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNZ49378.1"
                     /translation="MQTLKAKKRALGSGSIKTNMRLNGGVPSVLYGEAKEALPIEVEA
                     KAVEKLLREEGSSFIVLQLDFEDAPELNTPALVKEIQRHPVKSNLLHMDFMRVRLDER
                     IHLPVPVILVGRAQGVIDGGVIDQQLREVQVECLAAEVPEHLELDVTTLGLGGTLHVA
                     DLAVPEGVTVLTEAGYAIVSIHAPRTAKGDEEADAEDAEGGAADES"
     gene            complement(1932..2885)
                     /locus_tag="PKN92_11085"
     CDS             complement(1932..2885)
                     /locus_tag="PKN92_11085"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNZ49379.1"
                     /translation="MDFTNSFKVFSGSASKQLTEGICRHLGIDPGRALVGRFSDAEIH
                     MQIQEHVRGYDVFIVNSTAPPVNDNLMELLMFCDAARRASADRITAVIPYFGYARQDR
                     KDKPRVPITAKLVANLLTAAGANRVLTVDLHCGQIQGFFDIPLDHLAADVVFCEHLKK
                     EGVHKDLIVVSPDMGSVARARSFASRLGTPLAIVDKRRPKENEAEVMNIIGSVEGRHA
                     LMLDDMIDTGGTLVKAAAAIIDHGAQSVRACATHPVFSGPALDLLEASVLQEILVCDS
                     ISLSERAAACPRIKSLSLAPLIGEAIHRIHKDESVSSLFTV"
     gene            complement(2931..3006)
                     /locus_tag="PKN92_11090"
     tRNA            complement(2931..3006)
                     /locus_tag="PKN92_11090"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2971..2973),aa:Gln,seq:ttg)
     gene            3444..4403
                     /locus_tag="PKN92_11095"
     CDS             3444..4403
                     /locus_tag="PKN92_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNZ49380.1"
                     /translation="MKLTFPGLMLITMLCMLSLSVAAADKTPMKAMLLTGQNNHNWQL
                     SSPILKTIIEDTGLFIVDTVEAPPEGGDFSGFSPVFSDYAVVVLDYNGDDWPEKTRED
                     FLTYVQEGGGVVVYHAADNAFPEWPEFNEIIGLGGWGNRTEKHGPYVRWEEGHLVYDM
                     SPGPGGAHGKQHQFVVTHRDTEHPVTRGLPETWLHAQDELYHHLRGPAKNLHVLATAW
                     SDPETGGTGNHEPILFTVSYGKGRMFQTALGHAGNESPPPAMQCAGFIVTLQRGTEWA
                     ATGEVTQEVPVDFPTETQVQLRPKFKKDSSAAQTEAVKEDSTQ"
     gene            4637..5974
                     /locus_tag="PKN92_11100"
     CDS             4637..5974
                     /locus_tag="PKN92_11100"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNZ49381.1"
                     /translation="MAHRSTGIACALLFSLMACLPMAPAFSQPKQITPVICYDREFPP
                     EGDPTGSPVSVTPSLSDNKCIFAETFTFRDCSPDIPVQSREWYINGNLVERAQHFEWR
                     LPEGRHEVTLTLYINDESFSATEFITVVDVFPVYADFAPSPAAGTVPLAVSFINRSAA
                     FKWLFYWWDFGDGTTSEEREPRHIYEKPGTYTVCLSVLDLRDCKGVSKNAQACRRVTV
                     WDIGASDCEGNLLQDPGFEAGDMTAHWDASPLSSSQRLLCSQENCLEGSPYDGTYWAR
                     FYDRMTDDVLAFGQKLSLKPGRYLFSYWYQCSDQANGTLSFHFNQTAIDRLYFPKMHS
                     GGGYAQRSVLLTVSEEESEQACLLECVNPTLGGAPVCLDHFCLIPFRQQPAADRNNNW
                     EISEDEAEDAITNWQIKDASLSEALRAQYLFRRGGAYRYNEALPPPLCWEPAE"
     gene            6020..6259
                     /locus_tag="PKN92_11105"
     CDS             6020..6259
                     /locus_tag="PKN92_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNZ49382.1"
                     /translation="MTDTVNEQQGTVQWFDSKKGYGFIAIPDAPNVKVHYSDIEMEGF
                     RTLREGQTVFFVLQETEKGLKAVRVRPADTTEEPI"
     gene            6308..>7704
                     /locus_tag="PKN92_11110"
     CDS             6308..>7704
                     /locus_tag="PKN92_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49383.1"
                     /translation="MSAAQSFLYSILALFLPLSPCLYAQSGSVQESSVAVAVPLLSAP
                     INATWAANYGEVWFEKGFRGFVFEGILDDLRPFPAEESALIPERIPFEESNTGYLESL
                     TLTDFEEVETIPGDWDALQREISGASNRLRAAGITENFLALRFAPDAPWFTNPQWRRL
                     AESRFMLAGRFCHQTRLRGILLDSASDSAFFDYRWEGWPPLLEPATLAEEARRFGRKL
                     AVLFNKYCPDGVLLIRARPPESCGPLWFIFFEGVMEGMERSRNGAVVLFFQGSTDAPA
                     PAAYQALHERTERLWEGRLSSQAQEGWKRKGGLAFSLMPVAYQDDIPFARHPLEQYVP
                     SLYGAAVYGRRYVIIDAPEGGWWHIPPDVAEQFSGLYQKGRGRVTFAPPLPRSLDAFL
                     PKLLCSEARILGDLVVGEMPCTVLHREGKTILLAYDGIPEPMHWPLNTGMMTATNLIS
                     GKKLYYTPKERMVSL"
BASE COUNT         1815 a         2105 c         1951 g         1833 t
ORIGIN      
        1 gcgctagcca acgggtttac agcccgtccc ctttagccac tcgggcatcc caccaaagaa
       61 caatatacct tgacagggta tggcgaaaag tagtatacca aaaagaaaca gggaagatca
      121 aactgcaagc gtacaattat tcctgaactc ttatttgaga tgcggaattg ttggctgccc
      181 gcgccatggc aatttcgcct gtgcgcacca tttcgaggat accgtagccg cgcatcattt
      241 caagaaaagc ctgaatcttt tcctgagcgc ccgtcacttc cacagtaatg catttggcat
      301 ccagatccac aatcttaccg cggaatatct caacaatgcc cagcacttca ctgcgcactg
      361 tgcctgttgc cgctacccgg atcatgatca actcccgttc cacataggaa gcagcagtaa
      421 gatcttccac tttaatgacg tccaccagtt tattcaactg ctgcataatc tgagcaagga
      481 cgctgtcgtc gcctcttaca atgacagtca tacgggaaac agtcgggtca tccgtttctc
      541 ctacacacag actagaaata ttgtagccgc gggcactgaa catgcctgac acacgggcga
      601 gcacaccgaa ctgattggca acgaggacac aaatagtatg ttttttctct gtcatgattt
      661 ttctcctggt gcgtcgtttc gccggttata acggttcata gccgtttcaa taccttcttc
      721 cagccagcac cgtgcggcct cggctgcttc gcgcaccatc ttttcgacat cagaaatttc
      781 ccagggtgcg aattttccta acacataatt gctgagtcta tcgggcgctt cggcatgatt
      841 tccaatgccc agccggagcc gatgaaactc ctgcgatccc agatctgcga ccagcgattt
      901 taatccatta tgcccgccgg ttccgccgcc tgcccgcaaa cgcagcacac ccagaggcag
      961 atgccgatca tcagaaacgg ctagaatatc ttcaggaagg catccggcac gcctcgccag
     1021 cggtgcgaca gcctgaccgc tcaaattcat aaaagtctga ggcttcacca gcatcacaga
     1081 cgtgccacga tgctgaaaac gcaccgtatg ggctttgtcc cgggttgccg aaatctcaac
     1141 attcagcaga gcggcaagcc tgtcaataac gacaaacccc agattatgcc gcgtgttgcg
     1201 gtattgttct ccggggttgc caagtcctgc aataagtttc atcagggtgc cttgtttgaa
     1261 aggggacgca gacgaggcgc cggcactcag ctttcgtcag cggcaccgcc ttccgcatct
     1321 tctgcgtcgg cttcttcatc acccttcgcc gtgcggggcg catgaatgga aacaatggca
     1381 tagcccgctt cagtgagaac agtaacacct tccggaacag ccaggtctgc cacatgcagt
     1441 gtgccaccca gtcccagagt ggtaacatcc agttccagat gttcgggcac ttcagccgcc
     1501 aggcattcca cctgcacttc gcgaagctgc tgatcaatga cgccaccgtc aatgacgccc
     1561 tgtgcacgcc ccacgagaat aacgggaaca ggcaggtgga tccgttcgtc gaggcgtacg
     1621 cgcatgaaat ccatgtggag caggttgctt ttcaccggat gccgctgaat ttccttaacg
     1681 agagcgggtg tgttaagttc cggcgcatct tcgaaatcga gttgcagcac gatgaaactg
     1741 ctgccttctt cgcgaagcag tttttcaacg gcttttgctt cgacttcaat gggaagcgcc
     1801 tcttttgcct ctccgtaaag gactgagggg acgccgccat tcagacgcat gttagttttg
     1861 atacttccgc tgccaagagc gcgttttttt gctttcaaag tctgcatttg ggtttgacct
     1921 ccgaatcggg atcacactgt gaacaggctg cttaccgatt catctttgtg aatacgatga
     1981 attgcctctc cgatgagcgg tgccagcgac aagctcttga tgcgaggaca cgcggccgcc
     2041 cgttcagaca gggagataga atcacatacc aatatttctt gtaaaacaga tgcttccagc
     2101 agatcaagag ccggtccgct gaatacggga tgggtagcgc aggcgcgaac gctttgcgca
     2161 ccatggtcta tgattgcggc ggccgctttt accagcgtgc cacctgtatc aatcatatca
     2221 tccagcatca gcgcatgccg accttcgaca ctgccgataa tattcattac ttccgcttcg
     2281 ttttccttgg gacgacgctt gtccacaatg gcaagaggcg tacccaacct gctggcgaag
     2341 cttcgggcac gtgcaacact gcccatgtcg ggtgacacaa cgatcagatc tttgtgcaca
     2401 ccttcttttt tcaaatgctc acaaaagacc acatccgccg ccagatgatc cagcggaata
     2461 tcaaaaaatc cttgaatctg accgcagtgc aggtcaaccg tcaatacccg attggcaccg
     2521 gctgcagtga gcagatttgc caccaattta gcggtaatcg gtacacgcgg cttgtctttt
     2581 ctgtcctgac gggcataccc gaaataaggg atcactgctg taatccggtc tgccgaagcg
     2641 cgccgcgccg catcgcagaa catcagcaat tccatcaggt tatcatttac cggaggggct
     2701 gtgctgttca cgataaacac gtcgtaaccg cgcacatgct cctgtatttg catatgaatt
     2761 tccgcgtcac tgaaacggcc taccagtgcc cgcccgggat cgatgccaag atgccggcaa
     2821 atgccttctg tcaactgttt cgaagcactg ccactgaaca ccttgaaact gtttgtaaaa
     2881 tccaccgcac tgcgcctttc gtatcagatg catggaccct gaaataaatt tggctggggc
     2941 ggctggactc gaaccaacga tagccagatc caaaatctgg tgccttacca acttggctac
     3001 gccccatcac aaaagaaatt aagaaaaata gtacacgagt tggagtcttg atcagggggt
     3061 tgaagtatac caactacctg tgtcttttgt caaaaattcc gttgtcgggc aataaaaacg
     3121 atcctgcagg agtctattat tcgtttttta cgcacattat ggctgcaaac ggacagcgct
     3181 gccgttccgc caacaactaa agagaaggca gcagaatgcg tcattttcca ttttttttgc
     3241 gcccccgttg cccttatccc ctgcttcaca ttttccaaat atgcattacc ctcaaatctt
     3301 tttgtatgat aaaaatcttg cgggcattcg ccgactagtg atcctggtgc ggattgaatg
     3361 ctctgctgcc ccatgcgtgc atcgtgtgcg caggggtacg ctctatatgc gacgataccg
     3421 taaacctatt ggagactctc tttatgaagt taacttttcc cgggctgatg cttatcacga
     3481 tgctttgcat gctctccctg tccgttgcag cagccgacaa aacacccatg aaagccatgc
     3541 tgctcaccgg acagaataac cataactggc agctaagctc ccccattctc aagacaatta
     3601 ttgaggacac aggacttttc attgtggaca ctgtcgaggc acctcctgag ggcggcgact
     3661 tctccggatt ttctccggtt ttctccgact atgccgttgt tgttctggat tacaacggcg
     3721 atgactggcc ggaaaagact cgtgaagact ttttaaccta tgtgcaagaa ggcggtggcg
     3781 tggtggttta ccatgcggca gacaatgctt ttccggaatg gcccgaattt aatgaaatca
     3841 tcggtctcgg cggctggggc aatcgtacag aaaagcacgg cccctatgtc cgctgggaag
     3901 aaggtcacct ggtgtatgac atgagtcccg gccccggcgg agctcacggc aagcagcatc
     3961 agtttgtcgt gacacaccgc gatacagaac atccggtcac cagagggctg cccgaaacct
     4021 ggctccatgc acaggacgag ttgtaccatc acctgcgggg ccccgcaaaa aacctgcatg
     4081 tactcgccac ggcctggtct gatcctgaaa caggaggaac aggcaatcat gaacctattc
     4141 ttttcaccgt atcctatgga aaagggcgca tgttccaaac cgcactggga catgcgggca
     4201 atgaatcgcc gccgcctgcc atgcagtgtg ccggttttat cgtaacactg cagcgaggta
     4261 ctgaatgggc agccaccggc gaagttacgc aggaagtgcc tgttgatttc ccgaccgaaa
     4321 cccaggtgca gctgcggccg aaatttaaaa aagattcttc cgccgcgcag accgaagcag
     4381 tcaaagaaga ctccactcag tgaccggcgg agaaatgaaa aaagagcgaa tgccttacca
     4441 ggaaaaacga ctctgcgacg tgccgacgaa agaaacggaa ggcctgcgac gtagcctgcc
     4501 ggtctcaaat ttctattgac acccgccggg agcgggtata taatcatgaa aggaaatcgt
     4561 tcgttatcct gccggcagca ggatgaaaaa ggctgtcttc cgctcgttac cgggctgttc
     4621 gtcaaggagt aacgccatgg cacaccgttc aaccggaata gcgtgcgcct tacttttttc
     4681 tctgatggca tgtctgccga tggctcccgc cttttcccag ccgaaacaaa tcacaccggt
     4741 aatttgctat gaccgtgaat ttccgcccga aggagaccca accggaagtc cggtttccgt
     4801 tacgccttca ctctccgaca acaaatgcat ctttgccgag accttcactt tccgggattg
     4861 ctctcccgac atcccggttc aaagccgtga gtggtatatc aacggaaatc ttgtcgagcg
     4921 tgcacagcat tttgaatggc ggcttcctga agggcggcat gaagtcaccc tgaccttata
     4981 cataaatgat gaatcttttt ccgccacaga atttatcact gttgtggatg tctttcctgt
     5041 ttatgccgat ttcgcaccga gtccggcagc agggacggtg ccgctggctg tgtcgtttat
     5101 caaccgttct gccgcattca aatggctctt ctactggtgg gatttcggtg acggtacaac
     5161 gagtgaggag cgtgaacccc ggcatattta cgaaaagccg ggaacgtata ccgtctgtct
     5221 ttccgtgctg gatctgcgtg actgcaaagg cgtgtcaaag aatgcgcaag cctgccgccg
     5281 cgtaactgtt tgggatatcg gcgcatccga ctgtgaagga aatctgctgc aggatcccgg
     5341 ttttgaggca ggagatatga cagcgcactg ggatgcctcg cctctttctt cttcacagcg
     5401 tcttttatgc tcgcaggaaa attgcctgga aggaagtccc tacgacggca catactgggc
     5461 tcgcttttac gaccgcatga ctgatgatgt gcttgcgttc gggcagaagt tatcgcttaa
     5521 acccggcaga tacctctttt cctactggta tcaatgttcc gatcaggcaa acggcacgct
     5581 gtcctttcat ttcaatcaaa cggcaataga tcggctctat tttccgaaaa tgcattccgg
     5641 gggtggctat gcgcagcgct ctgttctttt aactgtaagc gaagaggaaa gcgagcaggc
     5701 ctgtcttttg gagtgtgtaa atcccacgct gggcggtgcg cctgtatgtc tggatcactt
     5761 ctgtcttatt ccgttccggc agcagccggc tgcggatcgg aacaacaact gggaaatcag
     5821 tgaagacgaa gctgaggatg ccattacgaa ttggcagatc aaagacgcct ccctgtcgga
     5881 agcgcttcgg gcgcagtatc tgttcagaag aggaggggca taccgctata atgaagcgct
     5941 gccgccgccc ctttgctggg aaccggcaga atgagttccg gcaggaaatt ggaagaaaac
     6001 agggaaagag ggacacatcg tgacagacac agtgaatgaa cagcagggca ctgtacagtg
     6061 gtttgacagc aagaaagggt atggttttat cgccattcct gatgcaccga atgtcaaggt
     6121 tcattactcg gatatcgaaa tggaagggtt ccgcaccctt cgggaaggac agactgtttt
     6181 ttttgtgcta caggagactg aaaaaggttt gaaagccgtt cgggtacgtc ctgcagacac
     6241 gacggaagaa ccaatataaa gcacacggca gcgtctgcta tagtatccgt caggatatct
     6301 ttcagacatg agtgccgcac aatcttttct atactccatc ctagctctgt ttcttccgct
     6361 ttctccctgt ctttatgcac aaagcggcag cgttcaggaa tcatctgttg ctgttgctgt
     6421 gcctcttctt tccgccccca tcaatgcaac ctgggccgcc aattacggcg aagtctggtt
     6481 tgaaaaagga ttccggggct ttgtctttga gggcattctg gatgatctgc gcccgttccc
     6541 tgccgaagaa tctgctctga tccccgaacg gatccctttc gaagagtcca ataccggata
     6601 tctggaaagc ctgacactga cggattttga agaggtagaa acgataccgg gagactggga
     6661 cgcactgcag cgggaaattt ccggagcttc caatagactc cgtgccgccg gtatcacaga
     6721 aaattttctt gctttgcgat ttgcacccga tgccccctgg ttcacaaatc cccagtggcg
     6781 acggcttgcg gaatcccgtt ttatgctggc ggggcgcttc tgccatcaga cacggctccg
     6841 gggaattcta ttggacagtg cttcggacag tgcttttttt gattaccgct gggagggctg
     6901 gccaccgctg ctggagccgg ccacactcgc ggaagaagcg cgccgcttcg gcagaaaact
     6961 ggcggttctt ttcaataaat actgtccgga cggcgtgctt ctgatccgtg cccgaccacc
     7021 ggagtcctgc ggtcctttat ggtttatatt ttttgaaggt gtgatggaag gcatggagcg
     7081 atcccgcaat ggcgccgtag ttttattttt tcagggaagc actgatgcgc ctgcacctgc
     7141 cgcctatcag gctttgcatg agcggacgga acggctctgg gaagggcggc tttcgtctca
     7201 agcgcaggaa ggctggaagc gaaagggagg cctggctttt tccctgatgc ctgtcgctta
     7261 tcaggatgac atcccttttg cccgacatcc cctggagcag tatgtgccgt ccctctacgg
     7321 cgccgctgta tacggcaggc gttatgtaat catcgatgcg ccggaaggcg gatggtggca
     7381 cattccgccc gatgtggcag aacagttctc cgggctgtac cagaaaggcc gcggacgggt
     7441 taccttcgca ccgccactgc ccagaagtct ggacgcattt ctccccaagc tgctctgcag
     7501 tgaagccaga attcttggcg atcttgttgt aggagaaatg ccctgcactg tattgcatcg
     7561 ggaagggaaa accatacttc tcgcctacga cggcattcct gaacctatgc attggcccct
     7621 gaatacaggc atgatgaccg ccacaaatct gataagcggc aaaaaactgt actatacacc
     7681 caaagagcgc atggtatcgt tgca
//
LOCUS       DAOPWB010000142         7521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_9807_length_7521_cov_4.555719, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000142 DAOPWB010000000
VERSION     DAOPWB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9807_length_7521_cov_4.555719"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PKN92_11115"
     CDS             complement(<1..641)
                     /locus_tag="PKN92_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49384.1"
                     /translation="MSKRAEKENQTPRKAEQQPLVTVLVVWLFLTAVFLLFNLVYLPR
                     YRQQMLAAPESFMQRARWLAKQGERARAKETLREGISLFNPPAPEPYQALASLLDDAD
                     SAREKVLTAGRSAFYQLTGTDCSAEKMTALGMLGGAALFPKHYDQALKPLSGALDISW
                     QSFSGALGLSTPPEWGIPERALVFLLAGSAIDFSGNIGTTGVKTPVPLLVYSGG"
     gene            complement(767..3853)
                     /locus_tag="PKN92_11120"
     CDS             complement(767..3853)
                     /locus_tag="PKN92_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ49385.1"
                     /translation="MSLPDLSVRRPVAMSCLIIALAILGVNAYRKMGLELMPSVDIPY
                     ITVITVYPGATPEEIETDIAKRIEDKMVTIAGLKHVSSTCIENACQTLLEFRLGIDVD
                     IAATDVREKLDLVRNDFPEDVEDPIVMKFDVNATPIITLALTGDATVEELFDYGDNTL
                     RDRLTVIPGVADVTLVGGAKREVHVQLDRDKVAARGLTSLHVVEAIKSGVRTIPAGRI
                     RSDGSEYVVKFDGDMQTVDAINDLEITGDNGSRCYVRDVGRAFMTTKEQREAAFFDGT
                     PAISIQVVKRSEANAVAVVDEVKKAIALINEELPGGMVLEWVTDDGTFTRAVIDSAWS
                     DVIQGVILTAIILFLFLYNIRSTFIVAVTMPLTLVIGLFFIQMLGYTLNTMTLLSMGL
                     SVGILVTNSIVVIESIVSGLNAGLPPKEAARVGSKEVFVAVLASASTNMVVLFPLAVM
                     KTMVGLFIGPFVWTMLIVTVVSLFISFTLTPLLASLILRPVKKDSKHPLVYMQRAWDW
                     MFGLVVKMYRGVLVLTERYRPLALLVVLGIGFLFVFTLGQAKKLGMSLGESPDRGELF
                     VKAEFPTYYSMETTLGRLKEMEALVQTLPHLRHYVSRVGKVEAGFGRSSDGVYLAQIL
                     LRFNERTEREETIEDLMEEVRRRMANFPDCIQSVAQPSFVGGQEAPIQLEVSGMDLTV
                     LDSLALKIAQKTAEAPGFVDLDTSVRSGKPELRVRPDRTVLSDMGIPPLQLGMMLRGN
                     LEGITAGTYKQDARNYDIVVKFSDEEGKSQIRDFLLPAAPGRPIPLNTVGSVQESTTP
                     VQIPRKDKKRVAKVFADLSHDLSLGDGVEEIERIIETNNLLPPGYNHNFAGEYEIMKE
                     GQTEMAEAGLIAIILVILTLAALLESFKQPFLILVTLPIGLIGVIWALLFAGHAMSIF
                     VLMSVVMLIGIVVNNAILIVDQFNQMVAKGVHRHAAMIDAACDQFRPIIMITLAGILG
                     MMPLALGTGIGAEMRNGVGIASVGGMFASGILSLILVPVLYDLFTRKAKNGKKPQD"
     gene            complement(3853..5037)
                     /locus_tag="PKN92_11125"
     CDS             complement(3853..5037)
                     /locus_tag="PKN92_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ49386.1"
                     /translation="MKFEKLTCYGSTALLLLALPFGGCSSRPTAAASEEAAKRVSIRT
                     TPAEVRLFEKTIAAQGTLNAKRTAMVPPLIDGTITSFFVEVGDAVKAGETKLFQIDKI
                     KVERALAVSEQNLALARSGQKDAEAQLASVQAQYDKAHLDYERYTRLYDQKAITPDAM
                     EKAESGFKVASAGLERARVGVQAASEQLVQAEASVEISRKTLKDSLIYAPMDGIVSAR
                     LKEEGEFGAAGAPLIRIVDPGLLELSAFLPGEYYAEIIPGETQLQLSVQGIDLGVYPV
                     SFKSPEIQAKLRNFEVRCLVPDPPAGVAPGAEAKLSAVLFAREGVGIPSTAIQRRGGK
                     EVVFTVSENRTRGIEIVRGLESAGWTEITNDALTAGMQIVTMGQFLVEDGTQVSLSEE
                     GA"
     gene            complement(5034..6428)
                     /locus_tag="PKN92_11130"
     CDS             complement(5034..6428)
                     /locus_tag="PKN92_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNZ49387.1"
                     /translation="MPSKTMKILLFLLLCLVSAGCARFNPEAVRSEHALSFSAALEAR
                     TERVLSESGALNLHQCIDLALENNLNVQAADIEARIARLNRQVAFSHFLPSIEMSYTS
                     TELNKTPSSSMFGPFAVSVQDRIVRETMVKAQMPVFAPATWFLYDAHRHGEAIGTLVS
                     DYARQMIALQVTGLYFQCLALEEAGKALASQHASARVLCGEVEAFYGAGLVMESDVLQ
                     ARTLVLSRGRDCEKNMRELAAARSGLLEVMGLSPLASLTLDAETPVELPEGETADWIM
                     HALLHHPRLAIADRQAAIEEDKLKIAITEFLPVLALFSARNHTTNSKNTYPYMTALGF
                     TGMMTVFNGFANINEYKAARLHKKGAYLALERESLSLMAQVYQSRLQVDNAESDSVLA
                     AAALEAAEAALREAEVRFHEGVINTSDVMEARSRRDSAASFVIAAEYQKQVTAAVACN
                     VMGATFRGDKVSSL"
     gene            complement(6418..7092)
                     /locus_tag="PKN92_11135"
     CDS             complement(6418..7092)
                     /locus_tag="PKN92_11135"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HNZ49388.1"
                     /translation="MDENIPATKGILLETAGELFANAGLEGVSIRKIAQKAGANIAAI
                     HYHFGSKDNLYLEVVRHVMHRIRCTLAGQYLADRAAWQDNPLSRAKVICALVEERMVQ
                     FFPDPHPGWYGRLFMRMLLNPTPALGKLAEEIILPDFEQLTEVFRLCKPGMSLSEARC
                     WTDTLLGQLGHYVFSAQFVQYMPNQNRQDADFQNMISRHLSRLLLKGLDLPLPDFLKD
                     DTLHAK"
     gene            7322..>7521
                     /locus_tag="PKN92_11140"
     CDS             7322..>7521
                     /locus_tag="PKN92_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ49389.1"
                     /translation="MKKRVITWVIGLLVLSSIAVGVAIAASDTPATDVEAVGAPQIAV
                     VDSAAIDGAGGAGGAAGGAGGGA"
BASE COUNT         1853 a         2164 c         1866 g         1638 t
ORIGIN      
        1 ccgccgctgt acacaagaag cggaacaggt gtctttacgc cggtggtccc gatattgccg
       61 gaaaaatcga ttgcgctgcc tgccagcaga aaaaccaggg cacgctccgg aatgccccac
      121 tcagggggcg tggagagccc cagcgctccg gaaaagcttt gccaggaaat atccagtgcg
      181 ccgctaagcg gtttcaacgc ctgatcatag tgcttcggaa aaagggccgc cccgcccagc
      241 atacccaatg cggtcatttt ctccgcagaa caatcagtac ccgtcaattg ataaaaagcg
      301 gatctccctg cggtcaatac tttttctctt gcactgtccg catcatcaag aagtgacgcc
      361 agtgcctgat agggttccgg agcgggaggg ttgaacagag aaatcccctc acgaagcgtc
      421 tcctttgcac gcgcccgttc tccctgtttc gccagccatc gggcacgctg cataaaagat
      481 tcaggtgcgg caagcatctg ctggcgatac cggggcaggt acaccagatt aaaaagcaga
      541 aacacagcag tcagaaagag ccagacaacc agcactgtaa ccaggggctg ctgctctgct
      601 ttccgcggcg tctgattttc tttttcggct cttttactca tgggtacatc ttagaccaga
      661 aagcaggaaa ataccaagaa gaaaaggctg ttatgccttg aaaagcgaac cccggcagcg
      721 agaagcagta tctccctgcc ggggccgttt ttcaatctgc taaaaatcag tcctgaggct
      781 tctttccatt ctttgctttt cgcgtgaaaa gatcataaag caccggcacc agaatcaggg
      841 acaaaatacc ggaagcgaac atgccgccca ccgaagcgat gccgacaccg ttgcgcattt
      901 ccgcaccgat acctgtgccc agcgccaaag gcatcatgcc cagaatgcca gcaagcgtaa
      961 tcatgatgat gggacggaac tgatcgcatg ccgcatcaat catggctgca tgcctatgca
     1021 ctccttttgc aaccatctga ttgaactgat ccacaatcag aatggcgtta ttcaccacga
     1081 tcccgatgag catgaccacg ctcatgagca caaaaataga catggcatgc ccggcaaaaa
     1141 ggagcgccca gatcacaccg atcagtccga tgggcagtgt gacgagaatc aggaagggct
     1201 gcttgaagct ttcaagcagt gcagccagtg taaggatgac cagaataatg gcaatcagac
     1261 ctgcttccgc catttccgtc tgaccttcct tcattatctc atactctccg gcaaagttat
     1321 ggttgtagcc cggaggaagc agattgttcg tctcaatgat ccgctcaatt tcttcgacac
     1381 cgtcacccag tgaaagatcg tgggaaagat cggcaaagac ctttgccaca cgttttttat
     1441 ctttacgcgg tatctgcacc ggtgtggtgc tttcctgaac cgagcctacc gtattcagcg
     1501 gaatggggcg accgggcgct gcaggcagaa gaaagtcccg aatttgactc ttgccttcct
     1561 catcggagaa cttcaccaca atatcatagt tccgggcatc ctgcttataa gtgccagccg
     1621 tgatgccctc caggttgcca cggagcatca tgcccagctg cagaggaggt atgcccatat
     1681 cggacagcac cgtccggtcg ggtcgcacac gcagctcagg tttgccggac cggacactgg
     1741 tatccagatc aacgaatccc ggggcttccg ctgttttctg cgctatcttc agtgccagcg
     1801 agtccagaac cgtcagatcc ataccggata cttccagttg aataggcgct tcctggccgc
     1861 ctacaaaaga cggctgcgcc acgctctgga tacagtccgg gaaattcgcc atgcggcggc
     1921 gaacttcctc catgaggtct tcaatggttt cctcgcgctc cgtgcgctca ttgaaacgaa
     1981 gcagaatctg cgccagatac acgccgtcgg aagaacggcc gaaacccgct tccaccttgc
     2041 ctacacgcga cacataatgg cgcagatgcg gaagtgtctg cacaagcgcc tccatttctt
     2101 tcagtctgcc cagggtggtt tccatgctgt aataggtggg aaattccgcc tttacaaaca
     2161 gctccccgcg gtcggggctt tcaccaagcg acatgcccag cttttttgcc tggcccaggg
     2221 taaatacaaa aaggaaccca atgcccagca caaccagaag cgctaaagga cggtatcttt
     2281 ctgtgagaac aagaacaccg cgatacattt tcaccacaag accgaacatc cagtcccagg
     2341 cacgctgcat ataaacaagc ggatgctttg aatctttttt aaccggacgg aggatgagcg
     2401 atgccagcag cggtgtcaac gtaaaggaaa taaaaagaga caccacagtc acgataagca
     2461 ttgtccacac aaaaggcccg ataaacaggc cgaccatggt cttcatcaca gccagaggaa
     2521 acagcaccac catattggtc gatgcgctgg caagcacggc aacaaaaact tctttcgacc
     2581 ctacacgcgc cgcctctttg ggaggaagtc ccgcattcag cccgctgacg atggattcaa
     2641 tcaccacaat agagttagtc acgagaatac ctaccgacag acccatggac agcagggtca
     2701 tggtattcag cgtatacccc agcatctgga tgaagaaaag cccgatcaca agcgtcagtg
     2761 gcatggtcac ggcgacaata aaagtagatc ggatgttgta caggaaaagg aaaagaatta
     2821 tcgccgtcag aattacgccc tgaatcacat cgctccaggc actgtcgatc acggcgcggg
     2881 taaaggtacc gtcatcggtg acccactcca gcaccatgcc gcccggaagt tcttcgttga
     2941 tcagcgcaat ggctttcttc acttcatcca ccacggcaac agcattggct tcagagcgct
     3001 tcaccacctg aatggagatt gcgggggtac cgtcgaaaaa ggcggcttcg cgctgctcct
     3061 tggttgtcat aaaagcacgg cccacatccc gaacatagca gcggcttccg ttgtcacccg
     3121 tgatctccag atcattgatg gcgtcaaccg tctgcatgtc accgtcaaat ttcaccacat
     3181 attccgagcc gtccgaacgg atgcggcccg ccggaatggt gcgtacaccc gatttgatcg
     3241 cctccaccac atgcagtgag gtcagacccc gtgccgccac cttgtcacgg tcaagctgca
     3301 catgcacttc acgtttggct ccgcccacaa gtgtcacatc agcaacaccc ggaatcacgg
     3361 tcagccggtc acgcaaagtg ttgtcaccgt aatcgaacag ttcttccaca gtggcatcac
     3421 cggtcagtgc cagcgtaatg atgggcgtgg cattcacgtc aaacttcatg acaatgggat
     3481 cttccacatc ctcggggaag tcattgcgca caagatccag tttttcccgg acatccgtcg
     3541 ccgctatgtc cacatctata cccagccgga attccagcag tgtctggcag gcgttttcaa
     3601 tacaggtgga gctgacatgt ttcaagccgg caatggtcac cattttgtct tcgatgcgct
     3661 ttgcaatgtc cgtctcaatt tcttcgggcg tagcacccgg ataaacggtg atcaccgtga
     3721 tataaggaat atcaacggaa ggcatcagct ccaggcccat cttccggtat gcattcactc
     3781 cgagaattgc cagcgcgatg atcaggcaac tcatagcgac aggacgccgc acacttaaat
     3841 caggaagtga catcaggcac cctcctcgga caggctgacc tgcgttccgt cttccaccag
     3901 aaactgaccc atggtgacga tctgcatgcc cgctgtaagt gcatcgtttg taatttctgt
     3961 ccagcctgct gattccagtc cgcggacaat ctctatgccc cgtgtgcgat tctccgatac
     4021 ggtgaacacc acttctttcc cgccccgccg ctgtatcgcc gtggaaggaa taccgacgcc
     4081 ctcccgggca aagagcacgg cggacaactt cgcttccgca ccgggggcaa cacccgccgg
     4141 gggatcgggc acaagacatc tcacttcaaa attacgcagc ttcgcctgaa tttcggggct
     4201 tttaaaggac accggataaa cacccagatc gatcccctgc acactcagct gcagctgtgt
     4261 ttctcccgga ataatttcag cataatattc ccccggaaga aaggcggaca gctcaagcag
     4321 accgggatcc acaatacgaa tcaggggcgc acctgccgca ccgaactcgc cttcttcttt
     4381 aagccgtgcg ctcacgatgc cgtccatggg cgcatagatc agagaatctt tcagcgtctt
     4441 tctggaaatt tccacggaag cctccgcctg aacaagctgc tcagacgctg cctgcacacc
     4501 tacccgggca cgttcaagac ccgccgaagc aaccttgaat ccggactccg ccttttccat
     4561 ggcatcgggt gtaatcgctt tctgatcgta caaacgggta taccgctcgt aatcaagatg
     4621 cgccttgtca tattgtgcct gaacactcgc cagctgtgct tccgcatctt tctggcccga
     4681 ccgggctaat gcaaggttct gctccgatac ggcaagcgcc cgctctacct tgatcttgtc
     4741 aatctggaag agttttgttt caccggcttt gaccgcatcg cccacctcaa cgaagaaaga
     4801 agtgattgta ccgtcaatca gcggaggaac cattgctgtt cttttggcat tgagtgtgcc
     4861 ttgtgccgct atcgtttttt caaagagccg cacttctgcg ggcgtcgtac ggatagacac
     4921 acgcttagca gcttcttctg aagccgctgc tgtaggtctg gacgaacagc ccccgaaggg
     4981 aagtgcgagt aaaagcagcg ctgtgctgcc gtaacaggtc agtttttcga atttcataga
     5041 gaagacacct tatcaccgcg gaaagtagca cccatcacat tgcatgccac cgctgcggtc
     5101 acttgtttct gatattctgc ggcaatcaca aatgacgccg cactgtcccg acgggacctt
     5161 gcttccatta catccgacgt gtttatcacc ccttcatgaa aacgcacttc tgcttcacgc
     5221 aatgccgcct ccgccgcttc gagggctgca gctgcaagca cagaatcaga ctccgcatta
     5281 tcaacttgca ggcgggactg atacacctgc gccatcaacg acagactttc ccgctccagc
     5341 gcaagatagg cgcccttttt gtgcagccgc gccgctttat attcgttgat attggcaaaa
     5401 ccgttaaaaa ctgtcatcat ccccgtaaat cccagagcgg tcatgtaggg ataggtattt
     5461 ttactgttgg tcgtgtgatt gcgcgcagaa aaaagagcga gcacaggaag aaattcggta
     5521 atggcgattt tcagtttgtc ttcttctatt gctgcctgac ggtctgcaat ggcgagccgc
     5581 gggtgatgga gaagggcatg cataatccag tccgccgttt ctccctcagg aagctcaaca
     5641 ggcgtttccg catccagtgt cagagaggca agaggcgaca accccatcac ttccagaagc
     5701 cccgaacgtg ccgcggcaag ttccctcatg ttcttttcgc aatcccgccc gcgggacaga
     5761 accagcgtac gcgcctgcag cacatcggac tccatcacaa gccccgcgcc ataaaacgcc
     5821 tccacttccc cgcacagcac tctggcagat gcatgctgtg aggccagtgc tttacctgcc
     5881 tcttccaggg cgagacactg gaaatacaga cctgtcacct gcagcgctat catctgacgg
     5941 gcatagtctg aaacaagcgt gccgatggct tccccgtggc gatgagcgtc ataaagaaac
     6001 caggttgccg gagcaaaaac aggcatctgc gctttcacca tcgtttcacg gacaatccga
     6061 tcctgcacgg aaacagcaaa aggtccgaac atggatgagg aaggcgtttt attcagttcc
     6121 gtggaggtat agctcatttc tatggacgga agaaagtgcg agaaggcaac ctgccggttc
     6181 agccgggcaa tacgcgcctc tatatccgcc gcctgcacat tcaggttgtt ttccagcgcc
     6241 agatcaatac actggtgcag gttaagtgcg ccggattcgg aaagcactct ttccgtacgg
     6301 gcttccaggg cagcactgaa agacagggca tgctccgacc gcaccgcctc aggattaaac
     6361 cgggcacagc cagcagacac cagacagagg agtagaaata aaagaatttt catcgtttta
     6421 cttggcatgg agtgtgtcat cctttaaaaa atcaggaagg ggcaaatcaa gaccctttaa
     6481 aagcaagcgg gacaaatgac gggaaatcat attctgaaaa tccgcatcct gacggttttg
     6541 attcggcatg tactgcacaa attgcgctga aaaaacatag tgtcccagct gaccaaggag
     6601 cgtatccgtc cagcagcgcg cctctgaaag agacatgccg ggcttgcaga gtctgaacac
     6661 ttcggtcagc tgctcaaagt cgggcagaat gatctcttcc gccaattttc ccagagcagg
     6721 cgtcggattc agaagcatac gcatgaacag ccgcccgtac cacccgggat gaggatccgg
     6781 aaaaaactga accatccgct cttccaccag cgcacaaatc actttggcac ggcttaacgg
     6841 attgtcctgc catgccgccc tgtcagcaag atactgcccg gcgagagtgc agcgaatacg
     6901 gtgcatgaca tggcgaacca cttccagata caggttatcc ttactgccga aatgataatg
     6961 gatggcggca atatttgcac cggctttctg cgcaatctta cggatgctca cgccctcgag
     7021 cccggcattg gcaaaaagct ctccagcagt ttctaacagt atgccttttg ttgccggaat
     7081 attttcgtcc atgtcgtttc tcctggtgcc gaccctctgc cccactggca cgctgaggga
     7141 ttcactggaa gagagtatac actaaacaat cgtttaaatc aaatgtttga tccgtgcgcc
     7201 aaaaaaagct ttacttttcg tagcttgtgg tgtataataa cgacaggtta tgttatgtca
     7261 ggtgttcgag acaaacctgc aagtgtgatt ttgaggccat aataatcaaa ggagaattgt
     7321 catgaaaaaa cgcgttatca cttgggtgat cggtctgctg gttctctcca gtattgcggt
     7381 aggcgttgct attgccgctt ctgatacacc tgccacggat gtcgaagcag tcggtgcccc
     7441 gcagattgcg gtggttgaca gcgcagcgat tgacggtgcc ggtggtgccg gtggtgctgc
     7501 gggcggtgct ggcggtggtg c
//
LOCUS       DAOPWB010000143         7454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_9915_length_7454_cov_5.640357, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000143 DAOPWB010000000
VERSION     DAOPWB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9915_length_7454_cov_5.640357"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKN92_11145"
     CDS             <1..516
                     /locus_tag="PKN92_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49390.1"
                     /translation="MPPHIAFGRILSDKFALSIHGGYSEGVRRTKRRENSIFLGVPLS
                     TRVSIRRYSTYLGVDLDYYPFKMAKLQQYTNWGERLKAAKPTLGIRINYNRTGYDARA
                     RMALGWFNRGVAVHLHNHWALPGITLVAGFDIPLNKRNVMAINAGYTWFKKQTHDFDG
                     PAFTLAWKYFF"
     gene            complement(518..1513)
                     /locus_tag="PKN92_11150"
     CDS             complement(518..1513)
                     /locus_tag="PKN92_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNZ49391.1"
                     /translation="MKEHRLLLFFLLLSAVVLCISPFLGMTATSLSGVFGRHADALES
                     VVFWRIRIPRVLTAYLAGGGLALSGMAFQALFRNPLATPFTLGVSGGAAFGAALYMRL
                     GLAIALPGIGGSTIAAFSGAVLVILLAYWLTRLQGGFATHTLLLAGVALSFFLSSMIL
                     ALQYSASLHDSFRLVRWLMGGLGMVGYDAVLSLFPLVATGAVVLFLLRPELDLLLLGD
                     DLAMSRGMELQRTRLLLFFSVSLMVGGIVSVCGPIGFVGMMVPHMCRLLAGTGHRTLL
                     PASFLLGGAFLTLCDTGARILFAPAEIPVGVITALLGGPFFLFLLFSRNTRRMLR"
     gene            complement(1510..2301)
                     /locus_tag="PKN92_11155"
     CDS             complement(1510..2301)
                     /locus_tag="PKN92_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ49392.1"
                     /translation="MTDAVFDIQNLTVVLGGKRILSALDFTLHAGESLVIVGPNGAGK
                     STLLKAMLRLVPVQSGEIYFKGRSLKDWSRRELAQHAAYVPQAGERAIPYSVYEFVLM
                     ARYPYLSTFAALSEKDHAIAAEAMAQAEVEKFAHRPLYNLSGGERQKVFIAAALAQCP
                     DILFLDEATAFLDYRHQTEVLSLITKLRRDQGVSVVSVTHDLNQGALAYDRVLALKDG
                     QKVFFGSPAELFRSDILENIYDTSFHFLEDSESGRSIVVPGKAVL"
     gene            complement(2298..3194)
                     /locus_tag="PKN92_11160"
     CDS             complement(2298..3194)
                     /locus_tag="PKN92_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNZ49393.1"
                     /translation="MRGVVIGIVLAGTLGISVLLHSYVQDTGSRSQSSSGFISLSPGL
                     TETLFALGAGDAVKGVTQFCLYPPEAQERVSVGGLLDPNYEALVALNPQLIFMTPYHR
                     DLRREMRRLGLSYEIVPQDTLPEIRESFLHVGVLCGHEEEGRKLVAALDRHMSAVSEL
                     VRDREKMRVLLVTGRNEHSGSLQEVYAVSTGSFLSDLLEIAGAENCVSGAVAEYPALS
                     VEGILHLDPEYIIELVEKDPQTALPKLMAPWQALSELQAVQNSRVKILGGSHFTIPGP
                     RITQVLDAFVAALHPDLKECVL"
     gene            3672..4043
                     /locus_tag="PKN92_11165"
     CDS             3672..4043
                     /locus_tag="PKN92_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49394.1"
                     /translation="MRTSPFILSVFCLIALMLPVTALASYSGGEGTAENPYLLASTAD
                     WLLLCQTGADWGKYFTVTDDLDFNGVSMIPLGSYEHPFTGTLDGKGHSFDNIRLDLAN
                     DLALFSRINNATILNLHLKKY"
     gene            4048..7128
                     /locus_tag="PKN92_11170"
     CDS             4048..7128
                     /locus_tag="PKN92_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HNZ49395.1"
                     /translation="MANANGAGLTVNADNSVIRACTIDGQINGFAACAGGFAAKGLGS
                     IFSNCQAKGEINANYSAGGIIGECSDNTFLACCSTAKVSNSGSFYYAGGLAGRASNST
                     LKNCYARGAVSGYYAAGLVGLASSANIENCYAAAPLYAESAAGGLISYGGNASVTASY
                     WDMTVSGITGSDGGEGAPTESMTYPYSELCYVGWDFSNVWAADTEGENDGYPLLRDDG
                     TETQAVLDLTKEAHKESFTYTGEVIPYTITLLNIGTAPVQNITVKDGLTGLSEPIAEI
                     LPGRQFILETQYTVVEDDLLRGSVENTAEAVGYDPDGNEVTAESTATVQGYIFQQMTL
                     TIEADADTLPGEGAEITYTLAVQNTGSMALTDVSVEDPLTGLIETIDRLDCMVPREFT
                     TRYLVAAQDVAVACVGNTASAKGKDVNGLEVSAQAIHYIFAGTDPGYCGGSGTEADPF
                     LICRTSDWIHLTQTTDDWDKHFALTDDLNFFGALIPSMGKDGSYFSGNLDGRGYSLKN
                     IRLAGGYIALIGSIQDCTIRDLHLENILVDGKYQAAGLAIMATQCTISGCTASGRCTA
                     ATYDAAGLVVHCGNSTIINCAVAAEVSGFENAGGIACVFLNGTCRNCFSTGKVNAAQY
                     NAGGLVASAEYADFLECYSTAEVTGDFQAGGLVGSFNNSNMSNCYAQGNVFGGEIGGL
                     IGSTDSWGEYRSTVSNCYAAGQVGSEHESRVRGGIIGIAYSGTDVTASYWDTDRSGIV
                     YSASGEGRINSEMTYPYAGNTFIGWNFDDVWAEDATQRNNGYPWLKRIPPPEAEPDFP
                     PEICLDFNAKPAGFQAGTDEIVPVQDALFRTGAFHLLSGDTITDGLLSAMETDYGLSL
                     HLDNISVGYVFGPAGNLPLCVSIYCKKVKDIVNLSVNGELRVVKDFAELYGTTVGGAL
                     IRAFPLKDGRTVLHLAGPVSSFSIGGQALSLEKICSLCNETETAAHPADTDGDNFIDI
                     GEAAAFVQDWQEDSDPMTSAIRAKYIAEKGGAYVFDPALPPPLCWIPESDIDLL"
BASE COUNT         1981 a         2002 c         1888 g         1583 t
ORIGIN      
        1 atgccgcccc acatcgcttt cggaagaata ttgtctgata aatttgcgct ctccattcat
       61 ggcggctatt cggaaggggt gcgccggacg aaaagacgtg aaaactccat ttttctgggc
      121 gtgccgctgt caacccgtgt aagcatccgc cgttacagta cctatctcgg tgttgatctg
      181 gattactacc ctttcaaaat ggccaaactg cagcagtata caaactgggg cgaacggctc
      241 aaagctgcca agcccacact gggcatccgc atcaactaca accgcaccgg ctacgatgcc
      301 cgggcacgca tggcgctagg ctggtttaac cggggtgttg ctgttcattt acataatcat
      361 tgggctttgc cggggattac acttgtggca ggtttcgata tcccgctgaa caaaagaaat
      421 gtcatggcga tcaacgccgg gtatacctgg tttaaaaagc agacccatga tttcgatgga
      481 ccggcattca ctctggcctg gaagtacttt ttctgaacta ccgcagcatg cggcgtgtat
      541 tccggctgaa aaggagaaaa agaaagaagg ggccgcccag aagggcagta atcacgccca
      601 caggtatttc ggcaggggca aaaagaatcc gagcacccgt atcgcagagc gtcaggaaag
      661 ctccgcccag caaaaaagaa gctggcaaca gagtcctgtg tccggtgccc gccagaagtc
      721 tgcacatgtg gggcaccatc atgcctacaa agccgatggg gccacagacc gacacaatac
      781 cgcctaccat gagggatact gaaaaaaaca aaagcagtcg ggtgcgctgc agttccatgc
      841 cccggctcat agccaggtca tcccccaaaa gaagcaggtc aagctcagga cgcagcagaa
      901 aaagcaccac agcccccgtg gcgaccagcg gaaaaaggct cagaacggca tcatagccaa
      961 ccattccgag cccgcccata agccagcgca caagccggaa agagtcatgc agactggcac
     1021 tatactgcag agccagaatc atgctggaca gaaaaaagct caaggcgaca ccggcaagca
     1081 gcagcgtatg tgttgcgaaa ccaccctgca gccgggtcag ccaatacgcc agaagaatga
     1141 ccagcacggc gccggaaaaa gcggcaattg tggagccgcc gatgccgggc agggcaattg
     1201 ccagaccaag acgcatgtat aaggcagcac cgaaagcggc gcccccggat acacccagcg
     1261 taaaaggtgt tgccagagga ttgcggaaga gtgcctgaaa cgccatgccg ctcagagcga
     1321 gcccgccgcc cgccagataa gcagtcagta cccggggaat gcgtatgcgc cagaaaacaa
     1381 cggactccag ggcatccgca tggcgcccga agacgccgga cagactggtg gcggtcatgc
     1441 ccagaaaagg cgatatgcag aggacaaccg ccgacagaag aagaaagaaa agcaacagcc
     1501 gatgttcttt catagcaccg cctttcccgg caccacaatg cttctgccac tctcggaatc
     1561 ctcaagaaaa tgaaaagagg tgtcataaat attttcgaga atgtcggacc gaaaaagctc
     1621 ggcaggtgat ccgaaaaata ctttctgtcc gtctttcaat gcaagaaccc ggtcatatgc
     1681 cagcgctccc tgattcaggt catgcgtaac agaaacgaca gaaaccccct gatcccgccg
     1741 cagtttggtg atcagcgaca gcacttctgt ctgatggcga taatcaagaa atgccgtcgc
     1801 ttcatccaga aaaagaatgt ccggacattg ggcaagggcg gcggcaataa acactttctg
     1861 gcgttcgccc ccgctgagat tatacaaagg gcggtgtgca aatttttcca cttccgcctg
     1921 cgccatcgct tcagcagcaa tcgcatggtc tttttccgaa agagctgcaa aagtgctgag
     1981 ataaggatat cgcgccatca gaacaaattc ataaacgctg tagggaatcg cccgctcccc
     2041 tgcctgaggc acataggcgg catgctgtgc cagttcccgg cgcgaccagt ctttcaggct
     2101 gcggcctttg aaataaatct ctcctgattg caccggaacc agacgaagca ttgccttcag
     2161 caatgttgat tttccggcac cgttcggtcc gacaatcaca agtgattctc cggcatgcag
     2221 cgtgaaatcc agtgcagaca gaattctctt tccgcccaga acaacagtca gattttgaat
     2281 atcaaaaacc gcatcggtca taaaacacat tctttcaagt caggatgcag tgccgcaaca
     2341 aacgcatcca gcacctgcgt aatgcgcgga cccgggatag taaaatggct gccacccaga
     2401 attttcaccc ggctgttctg aacggcttgc aattcagaaa gcgcctgcca gggagccatc
     2461 agcttcggca gtgccgtttg cggatccttt tccaccagct caataatata ctccggatca
     2521 aggtgcagaa tcccttccac agataaagcc ggatactccg ccaccgcccc cgacacgcaa
     2581 ttctctgcac ccgcaatctc cagcaggtcg ctgagaaagg agcctgtgct caccgcatac
     2641 acctcctgca ggcttccgct gtgttcattg cggcccgtta ccagaagcac ccgcattttt
     2701 tctctgtccc gcaccagctc cgacacagcc gacatgtgcc tgtccagagc agcaacgagc
     2761 ttgcgccctt cttcctcatg cccgcacaat accccgacat gcaaaaaact ttcacgaatt
     2821 tccggcaggg tatcctgcgg cacaatttcg taagacagcc ccaggcgcct catctcacgg
     2881 cgcaggtcac ggtggtaggg cgtcataaaa atcagctgcg gattcaaagc aaccagtgct
     2941 tcataattcg ggtccagcag cccgcccact gatacccgct cctgtgcttc aggagggtac
     3001 aggcaaaact gagtcacgcc cttcaccgca tctcccgccc ccagagcaaa taaagtctcc
     3061 gtcaaacccg gcgacaggga tataaatcct gagctgcttt gcgaccggct cccggtatcc
     3121 tgcacatagg agtgcagcag gacagaaatc cctaaagttc cggcaagcac aataccgatc
     3181 accactccgc gcacgaatca atactccttt cagaatgaag tccgtcattt cgcctattgt
     3241 acaggttcag ccggaaaaga atcacgcata ttttaactga aaaaaccgga ttgtacgggc
     3301 ttgccgctga agctggagga aaaagccgtt tctttcagcg tcttgcctgt tctcatgcct
     3361 cagaccgcta tgcagacggg gattttgaga cgagccgggc tggtgatgcc atcggcctta
     3421 acagtggtta tcttcgcctg tcagcggatt taatccatct gcacgcttgt acgtcgcata
     3481 aacctcttcc agcactctac ccatgtcaaa atattaatcc tgcaaaacct tgccatatag
     3541 acacacatct gatatactat tatttataga ataatatgaa aatttattcc ataaacccaa
     3601 caagagcgag gatgtaaaac agccaaagcc tggttgcgca cgcttcagga aatcaaggaa
     3661 gggatccacg catgagaaca tcgcctttca ttttatctgt tttttgcctc attgcactga
     3721 tgctgccggt aaccgccctg gcttcctaca gcggtggtga aggaaccgct gaaaacccat
     3781 atctcctggc atcgacagca gactggcttc ttttatgcca aacaggcgct gactggggca
     3841 aatatttcac ggtcacggat gatctggatt tcaacggcgt ttccatgata cctctgggct
     3901 cctatgaaca tcctttcaca ggaacgcttg acggcaaggg acatagcttt gacaatatca
     3961 gactggactt agccaacgac ctcgctctct tcagccgaat taacaacgcc accatactga
     4021 acctgcatct aaaaaaatat taaagtcgtg gcgaatgcga acggtgccgg gctcactgta
     4081 aacgccgata acagtgttat cagagcatgc acaatagatg gacagattaa cgggtttgca
     4141 gcctgcgcag gcggttttgc ggcaaaagga ttggggagta ttttttcaaa ttgccaggca
     4201 aaaggcgaaa tcaatgctaa ttattctgca ggcggcatca ttggcgaatg ctcagacaac
     4261 acctttttag cgtgctgttc gacggcgaaa gtaagtaatt caggcagttt ttactatgca
     4321 ggaggattgg caggaagagc atctaattcc acactgaaaa actgctacgc ccgtggagcg
     4381 gtgtcgggct attatgccgc agggctcgta ggcttggctt cttcagccaa tattgaaaat
     4441 tgctatgctg cggctcccct ctacgctgaa tcggcggctg gcgggctgat cagctatggc
     4501 gggaatgcct cggtaacagc atcttactgg gatatgactg tttcaggtat aaccggcagt
     4561 gacggcggcg aaggcgctcc gacggaaagc atgacctatc cctacagtga actctgctat
     4621 gtcgggtggg attttagtaa tgtatgggca gcagatacag aaggagaaaa tgacgggtat
     4681 cccctgctgc gcgatgacgg aaccgaaaca caggcagtac ttgatctgac caaagaggcg
     4741 cataaggaaa gcttcacata taccggagaa gtaattccct acacaattac actgctgaac
     4801 atcggtactg cccccgtaca gaacattaca gtaaaagatg gtctgaccgg tctttctgag
     4861 cccattgcgg agatcctgcc gggaagacag ttcattctgg aaacgcaata cactgtagtt
     4921 gaagacgatt tactccgggg ctcagtggag aatacggctg aagcagtcgg ctacgaccct
     4981 gacggcaatg aggtcacggc cgagagtacg gcaacggtgc agggttacat ttttcagcag
     5041 atgacgctga caatagaagc agatgcggac acgctgcccg gggaaggcgc cgaaatcaca
     5101 tataccctgg ccgtacagaa caccggaagc atggcgctga ctgatgtctc tgttgaagat
     5161 ccgcttaccg gtctgatcga aactatagac agactggatt gcatggtccc gagagaattc
     5221 acaacccgct atctggtcgc agcgcaggat gttgcagtcg cctgtgtagg gaatactgcc
     5281 tcagccaaag gaaaagatgt gaatggcctt gaagtatcgg ctcaggcaat tcactatatt
     5341 ttcgccggga ccgaccccgg atactgcggc ggcagcggta cggaagcaga tcctttcctg
     5401 atctgccgaa cctctgattg gattcatctg acacaaacaa cggatgactg ggacaagcac
     5461 ttcgcactga ccgatgacct caatttcttc ggcgccctga taccctccat gggcaaagat
     5521 ggatcttact tttcaggaaa tttggacggc cggggataca gcctgaaaaa tatcaggctg
     5581 gcaggcggct atatcgcgct gattggcagt atccaggact gcactatccg tgatctgcat
     5641 ctggaaaaca ttctcgtaga tggaaaatat caggctgcag ggctggccat tatggcaacg
     5701 caatgcacca tcagcggatg caccgcttca ggtcggtgca cagctgcaac ctatgatgct
     5761 gccggacttg ttgtgcattg cggcaatagc acgatcatca attgcgctgt agcagcggag
     5821 gtaagcggct ttgaaaatgc cggtggaatt gcatgcgtct tcctcaatgg cacctgccgc
     5881 aactgcttct ccacaggaaa agtgaacgcc gcccagtata acgcaggcgg gcttgtcgcc
     5941 tcggcggaat atgccgactt tttagaatgc tattccacgg ccgaagtcac aggagacttt
     6001 caggcgggcg ggcttgtagg ctcttttaac aattccaaca tgagcaactg ttatgcccag
     6061 ggaaatgttt tcggaggaga gatcggcgga ttgatcggct caaccgacag ctggggtgaa
     6121 taccgctcga ctgtttccaa ttgctacgct gcaggacagg tgggctccga gcatgaaagc
     6181 cgagtccggg gcggtatcat cggcatcgct tacagtggaa ctgatgtaac agcttcttat
     6241 tgggatacgg accgctcagg cattgtctac agcgcaagcg gtgaagggcg cattaacagc
     6301 gagatgactt atccttacgc aggaaataca ttcatcggct ggaactttga cgacgtgtgg
     6361 gcagaagacg caactcaaag aaacaacgga tatccctggc tgaagaggat tccgcctccg
     6421 gaagcagaac cggacttccc tcctgaaatt tgcctcgact tcaatgcgaa gcctgctggt
     6481 tttcaggcag gcacagatga aatcgtgcct gtacaggacg cactgtttcg aaccggagca
     6541 tttcatctgc tttccggaga cacaatcacc gatggtttgc tttcagcgat ggaaacagac
     6601 tacgggcttt cgctgcatct tgacaacatc agtgtcggct atgtctttgg tcctgccgga
     6661 aatcttccgc tctgtgtcag tatctactgc aaaaaagtaa aagatatcgt aaatctgagt
     6721 gttaacggag aacttagggt agtaaaggat tttgcagaac tctacgggac aaccgtgggc
     6781 ggagcgctta tccgcgcttt tccgctgaaa gacggaagaa cagtcttgca tctggcaggt
     6841 cctgtatcct ccttcagcat cggtggacaa gcgctttctt tagaaaaaat ctgctccctg
     6901 tgcaacgaaa ctgaaacagc tgcccatccc gctgataccg acggagacaa tttcatcgac
     6961 atcggcgagg cagcggcttt tgtacaggat tggcaggaag acagtgatcc tatgacttct
     7021 gcaattcggg ccaagtatat cgctgaaaaa ggcggcgctt atgtttttga cccggcgctg
     7081 cctccgcccc tctgctggat ccccgaatcc gatattgacc ttttatgaac aacgcgagag
     7141 agcaatacaa agatattgcc tactgtcata aaaaagcgct tgacaaattc gatactgtgc
     7201 agtatgctga gggtacggcc ttcgatctat acgatatgca gtacagcgta aaggtacatg
     7261 cgacatgtga agcactaccc gggcaggaga ttaaagacat gagacatgtg ctgattcaag
     7321 aaacaaagat cttagagccc agcgaatcta taaccacggg cttgaccgat aaaccaagcc
     7381 tctctgccgg gatacgggca gatcttcagt ctacctttaa taccgctatt accgaacacg
     7441 aagccaccat cccg
//
LOCUS       DAOPWB010000144         7356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10075_length_7356_cov_5.247226, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000144 DAOPWB010000000
VERSION     DAOPWB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10075_length_7356_cov_5.247226"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKN92_11175"
     CDS             complement(<1..392)
                     /locus_tag="PKN92_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNZ49396.1"
                     /translation="MYYGESFTILDGKKRLAIPATFRRVMEQEDHRVWYITRGYDRAL
                     FMFREETWEQLLKNLPNAPLDPQVLSFRRFFLGASIRVEPDAQWRIALSDGLKDFALL
                     ERECAILGVEDHLEIWNKSRWTEYSLQN"
     gene            832..1815
                     /locus_tag="PKN92_11180"
     CDS             832..1815
                     /locus_tag="PKN92_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ49397.1"
                     /translation="MKKNKQGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQ
                     NNLKQWGLVYKMYANESPGEKYPPMQFEARSHHNADIAIGPMVKSIYPEYLTDPSIAW
                     CPSDASSSLADLKDPDTGDFILHLHPDLIDTSYGYVGFLLDKCGDDQPDEAFISLGGV
                     FSIIPQLSNRFILDDPNASGPYQFVTLVLTTAGDAIARRSAGEDPVVASFQVVDKDKE
                     VSPVQTNSLGLIPMGNGSSNTINRLREGIERFLITDINNPAASARAQSDIWVMFDAIS
                     SNIKYFNHVPGGANVLYMDGHVEFLRYPSAKAPANKGMALFLGTLMDRTRG"
     gene            1843..2298
                     /locus_tag="PKN92_11185"
     CDS             1843..2298
                     /locus_tag="PKN92_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49398.1"
                     /translation="MKHYALGIALLFVLPPLLLLSGCGPSSEEKQVMAQFNAMDELIQ
                     RGGYIKSLEKFEKRDDRTFSIEAEIVDANGVPLGRLRSERVEGFATAKPRIQWYKTPG
                     EREEWNLPGWGGRRGGQGRGNRPDGAPRGGRGGRPAQDSGKAPAPTGTS"
     gene            complement(2310..3062)
                     /locus_tag="PKN92_11190"
     CDS             complement(2310..3062)
                     /locus_tag="PKN92_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49399.1"
                     /translation="MVFFLFLSLLGADEALSPAENEPAEKIYTREEWRPSIEATQEQL
                     ELLDLFAAATKFGGMKIKVPKELQSLPMRMIEGADELKDKGIELRVTELEFRNLKIVR
                     DATPQWQENRPRMPVRLEFDKLGVSADAKTKLGVIPVGAIFEQGTMPADFLPIIQKGF
                     NLEVLPEKRLQETNLDKVKLKVGGALTTGIANHFFSKKVARMILEYGVGQTLQMGQES
                     LMGGGIAGKLIDGGKDTPASRAAGSLIDQLLN"
     gene            complement(3095..6124)
                     /locus_tag="PKN92_11195"
     CDS             complement(3095..6124)
                     /locus_tag="PKN92_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNZ49400.1"
                     /translation="MNKKNLAIAVKLCVVAALFLLIFQPELFGLKGLFGDVAPLDVWN
                     ALKEASSTGPATFLFFMSCAVIVKLAGIFSGIIRWRLLLAGQGLKIPFTYLTYQWFMG
                     RAIGLFLPGTLGLDGFRLIESSRYTREVVKCTTVIAVEKLTGIIALALLVFLTFPLGF
                     KYLNINVAMLAIIMLCLLGGVICSLLLLLNPRVIQVIAAVMPVPNVARGLINKLGRAA
                     TAYSNSRRTLLTALFFGVLVHLGSCSKYFFTFMAIRAAHVSPADIFFVSPLMITASVL
                     AFTISGLGVREMAFGLVLGGSAGHAVAILGGHLGLWAGEILPFILSVPLLLFSGRPSR
                     DKLEEDRAYVEQNLADRQQALQPLLSEEEKTKYRKRLLEMLFCGTAAGLLAGAVIALL
                     EGAWILFHLQGLQEWYFLGWGTAAYSLLFAGLGCGIAGGLMFICLLFDRFMPWPVTTA
                     LIYTGTAALGGLVIGMFRYQRDILAGHAMTPRDLIMVAAFVLGTALILGILAFLKSEF
                     CRRFLRLQVPGFMAVCFLSWLLLITCAFAAGAFAAPQKESALMLTGTPAKGPNIILCA
                     ADAMRADCLPFYNPSITTETPNLTALTKDAILFKRAYAQAPWTKPSFATMFTGLYPGA
                     HGAASKTASLSADAPVVAGLLQAGGYFTRGFSNNPNTTALFGFDRGFSQFTDLKPDPL
                     FGAPQSAMHLSLYGLMRKVFLTAEGKLRGGRLRITDFYQPAEVVTDTALGWIDSRKEE
                     GDRPFYLYLHYMDTHDPFMDHADPGKGYARARMEHPDPDRYLEPMRQAYLSEIEYMDK
                     HLGRFFDGLKVRNLYDNTLIIFVSDHGEEFFDHQGWWHGQTLYEELLHVPLIMKFPGN
                     TQGGTVTDDLARLVDLAPTILQTAGLPLCPDMSGLPLTDKTGTPANSSIRYSYAENDF
                     EGNRLQALRTGERAVIQANENNTRGLAPVEFYNMVQDPLQQHNLAERSDAAEEVNSLL
                     EVIVQFMKAIADKAPLPSGETAIDPQLQQQLEALGYM"
     gene            complement(6282..>7356)
                     /locus_tag="PKN92_11200"
     CDS             complement(6282..>7356)
                     /locus_tag="PKN92_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49401.1"
                     /translation="SSWGLGSPGALRRLLSESSPVKRALFVLLILLLFIGLNYINYQQ
                     WPRVVKFFNLHKTAMAGWNTSFPGIIGLFLFGGLLSSVIVIVCIRYDNFLSAFRTAMK
                     IALPLFALIVLGALVQRGRHAFDHFTWNAFFTGVFGYVFWGFVQQLLFSAYLGTRLRK
                     AFAPTKNEGSATASGTKIVAGALIASLSFSLLLYIVITNSNGTVLPWQVIPGLTLFLF
                     PLSTLYLRFYFKDKKRMLVATLTGSCFGMIHINSYGLVAVTFLLGIFLSYVFMEERNR
                     NLVALGFIHGLLGSSFSQFFSKGRAGALNVDYGVGPWNVDNPGWETLIFPALCLLFYG
                     GIIVWYFRSASFHETETPRNLLS"
BASE COUNT         1917 a         1850 c         1956 g         1633 t
ORIGIN      
        1 atattttgta agctgtattc tgtccaccgc gacttattcc aaatctcaag gtgatcttca
       61 acaccgagaa tcgcacattc ccgctcaagc agtgcgaaat ctttcagacc gtcgctcaga
      121 gcaatgcgcc attgcgcatc cggctcaacc cgaatagagg cacccagaaa aaaacgacgg
      181 aatgaaagaa cctgaggatc gagtggcgca ttgggaagat ttttaagcag ctgctcccag
      241 gtctcttcac gaaacataaa gagcgcacga tcataccccc ttgtgatgta ccagacacga
      301 tgatcttcct gttccatcac acggcgaaac gtcgccggga tagccagccg cttcttaccg
      361 tcaaggattg taaaagattc tccgtagtac atgcgcttct tccctgaatt tccaaaatta
      421 tccaagtaac tccattttaa accactatca ggctaaaagt caataccttt tttgcttaaa
      481 aaaccgtttc tgcaagatca tttttgacta aaagggaaag ccactgtatg ttgtggatag
      541 aaacaataca cccactatag atagatggtc ttgtggaagg agaaagcagt cttctgaggc
      601 tgttttgatg agtcgggtgg agaaaagtgg aaaatggcgc acctgcagcg tatttacagg
      661 acgcaagggc ggctgtgctg cacctgaaac ccgttccgaa atgttcaagt ctttattaat
      721 agactgataa aaatgaaagt tttatattta aggttaattt atttttaaaa acatgcagtg
      781 aaaagcgtgt acagattcgg gaacaggttt cacgctctca aggagacagt tatgaaaaag
      841 aacaagcaag gctttaccct gattgaattg ctggttgtga tagccatcat cggtattctt
      901 gccgctattc tgcttccggc actggcacgg gcacgcgagg ctgcccgtcg ctcgagctgt
      961 cagaacaatc tgaaacaatg gggcctggta tataagatgt acgccaatga atctcctggc
     1021 gaaaaatacc ctcccatgca gtttgaagcc cggagccacc acaatgccga cattgccatc
     1081 ggtcccatgg tgaaatcaat ctatcccgag tatcttacgg atccttccat tgcctggtgc
     1141 ccctccgatg catccagcag cctggctgat ctgaaagatc ctgataccgg cgattttatc
     1201 ctgcatcttc atcccgacct tattgacacc agttacgggt atgtggggtt tcttctggat
     1261 aaatgcggcg atgatcagcc ggacgaagcc ttcatttcgc tgggcggcgt tttttccatt
     1321 attccccagt tgtccaaccg ctttattctc gatgatccga acgcatcggg gccctaccag
     1381 tttgtgacgc tggtgcttac gactgccggt gatgccattg cccggcggag tgcgggagaa
     1441 gatccggtag ttgccagttt tcaggtggtg gataaagaca aggaagtcag tcccgtgcag
     1501 accaacagtc tgggtctgat tcctatgggc aacggttcca gtaatacgat taaccgtttg
     1561 agggaaggta tagagcgttt tctcattacg gatatcaaca acccggcagc aagtgcccgc
     1621 gcgcaaagcg atatatgggt catgtttgat gcgatttcaa gcaatataaa gtattttaac
     1681 catgtgcccg gcggcgccaa tgtgctgtat atggatggtc atgtggagtt tctccgttat
     1741 ccgtccgcca aagcaccggc gaataaaggc atggcgctgt ttctcggcac gttgatggat
     1801 cgtacccggg gctgaaccct gtaagcagga aggaaactga atatgaagca ctatgcttta
     1861 gggatcgcat tattgtttgt gctgcctccg ctcctgctcc tgagcggatg tggcccttca
     1921 tccgaagaaa agcaggtcat ggcgcagttc aatgccatgg atgagctgat ccagcggggc
     1981 ggctatatta aaagtctgga aaaatttgaa aaacgggatg accgtacttt ttcaattgaa
     2041 gcggagattg tggatgccaa cggcgtgccg ctgggcaggc tgagatcgga gcgggtggag
     2101 ggctttgcca cggcgaaacc gcgcatacaa tggtacaaaa ctccgggcga acgcgaagaa
     2161 tggaatttgc ccggctgggg tggcagacgg ggcgggcagg gaagaggcaa tcggccggac
     2221 ggcgcacccc ggggaggtcg cggaggtcgc ccggcacagg attccggtaa agcgcctgcc
     2281 cccactggca cttcctgata ttcaggcact caattcagca gctgatcaat gagtgaacct
     2341 gccgcacggc tggcaggagt gtctttgcct ccgtcaatga gtttgcccgc aataccgccg
     2401 cccataaggc tttcctggcc catctgcagc gtctgtccca cgccgtattc aagaatcatg
     2461 cgggcaactt ttttgctgaa aaaatgattg gctatgccgg tggttaaagc gccgccgact
     2521 ttcagcttta ccttatcaag attggtctct tgcagccttt tttcgggaag cacttccaga
     2581 ttgaatcctt tttgaatgat cggcagaaag tcggcaggca tagtgccctg ttcaaaaatc
     2641 gcgccgacag ggataacacc cagtttagtt tttgcatctg ccgaaacacc gagtttgtcg
     2701 aattccagac ggacaggcat acgggggcgg ttttcctgcc actggggcgt tgcatcccgg
     2761 acaattttca gattacggaa ttccaattcc gtcacccgca gttcaatccc tttgtctttc
     2821 agttcgtcgg cgccttctat catgcgcatg ggcagcgact gcagctcttt cggcactttg
     2881 attttcatgc cgccgaattt ggtggctgca gcaaaaagat ccagcagttc cagctgttcc
     2941 tgagtggctt ctatggaagg acgccattct tcgcgggtat agattttttc ggcaggctca
     3001 ttctcagccg gagaaagggc ttcatcggcg ccaagaagag acagaaaaag aaaaaacacc
     3061 atggcatgta tccttgttag gcagaagcgt cggcttacat gtaaccgagt gcttcgagct
     3121 gctgctgcaa ttgggggtca atggctgttt cgcctgaagg cagcggtgcc ttgtcggcta
     3181 tagccttcat gaactggacg atcacttcga gcaggctatt cacttcttct gccgcatcag
     3241 accgttctgc cagattatgc tgctgcagcg gatcctgaac catattataa aattccacag
     3301 gagcaagccc gcgcgtgttg ttttcatttg cctgaatgac tgcccgttct ccggtacgca
     3361 gtgcctgaag tctgtttcct tcaaaatcat tttccgcata actataccgg atactgctgt
     3421 tggcgggggt accggtctta tcggtgagcg gtagtccgga catatcggga cagagaggaa
     3481 gcccggccgt ctgcaggata gtaggcgcaa gatccacaag ccgtgccaga tcatcagtaa
     3541 cagtaccgcc ctgagtattt ccggggaatt tcatgatcag gggaacatgc agcagttctt
     3601 catacagggt ctgtccatgc caccagccct gatgatcaaa aaactcctcg ccgtgatcgg
     3661 aaacaaagat gataagcgtg ttgtcgtaga gattccgcac tttcagaccg tcgaagaagc
     3721 gtccgaggtg tttatccata tattcgattt ccgacagata ggcctggcgc atgggttcca
     3781 gatatctgtc ggggtccggg tgttccatac gtgcacgggc atagcctttc cccggatcgg
     3841 catgatccat gaacgggtcg tgggtatcca tgtaatgcag atataaatag aaggggcggt
     3901 ctccttcttc tttccggctg tctatccagc caagagcagt gtcagtaacg acttcggcgg
     3961 gctgataaaa gtcggtgatg cgcagtctgc ccccccgcag tttgccttca gcagtaagaa
     4021 aaactttgcg catcaacccg taaagagaca gatgcatggc ggactgaggc gcaccaaaaa
     4081 gagggtcagg tttgaggtct gtaaactggg aaaagccgcg gtcaaagccg aagagagcgg
     4141 tggtgttcgg attgttggag aagccccggg tgaaatagcc gcccgcctgc agaagacctg
     4201 ccacaacagg tgcatcggca gagagggacg ctgttttgct ggcggcacca tgagcgccgg
     4261 gatacaggcc tgtaaacatg gtcgcaaagg acggcttggt ccagggcgcc tgtgcataag
     4321 cgcgtttaaa aaggatggca tccttcgtca gcgctgtgag attgggcgtt tccgtggtta
     4381 ttgacgggtt gtaaaaaggc aggcaatcag cgcgcatcgc atcggcggca caaaggatga
     4441 tattcggtcc ctttgcgggg gttccggtca gcatcagggc actttccttt tgaggtgcgg
     4501 caaatgctcc ggcagcgaaa gcgcaggtga tcagcagcag ccaggaaaga aaacatacag
     4561 ccatgaaccc gggcacctgc agacgcagaa aacggcggca gaattccgac ttcagaaacg
     4621 caagaattcc cagaataagt gccgttccca gaacaaaagc ggcaaccatg ataagatcgc
     4681 gaggtgtcat tgcatgcccc gcaagaatat cccgctgata gcggaacata ccgattacca
     4741 gaccgccaag tgccgctgtc cctgtataga ttagtgctgt tgtgacaggc cagggcatga
     4801 aacggtcaaa aagaaggcag atgaacatga gtccaccggc aattccgcag cccagccccg
     4861 caaagagcag gctgtaggcg gcagtgcccc atccgagaaa ataccactcc tgcagtccct
     4921 gcagatgaaa aagaatccag gcgccttcca gaagcgcaat gacggcgcct gccagcagtc
     4981 ccgccgccgt gccgcagaaa agcatttcca gaagtcgttt tctgtatttc gttttttctt
     5041 cttcgctcag aagaggctgc agtgcctgct ggcggtcggc aaggttctgc tccacatagg
     5101 cgcggtcttc ctccaactta tctctgctgg gtcgcccgct gaaaagcagc agcggtacgc
     5161 tgagaatgaa gggaagaatt tcacccgccc acaaccccag atgaccgccc agaatggcga
     5221 cggcatgccc ggcgctgccg ccaagcacaa gaccgaatgc catttcacgc actcccagcc
     5281 ctgaaatagt gaaggcaagc acagatgcgg tgatcataag agggctcaca aaaaagatgt
     5341 ctgccgggga cacatgggcg gcacggattg ccatgaatgt gaaaaaatat ttggagcagg
     5401 agcccagatg caccagtacg ccgaaaaaga gtgctgtgag cagggttctt ctggagttgc
     5461 tgtatgctgt tgccgccctg cccagcttat tgatcagtcc tctggcaacg tttggcacag
     5521 gcatcaccgc tgcgatcacc tgaatgaccc ggggattgag cagcagcaga aggctgcaaa
     5581 tgacaccgcc gagaaggcag agcataatga tcgcaagcat tgccacattg atgttcaaat
     5641 atttgaagcc aaggggaaaa gtcagaaaga ccaggagcgc cagtgcaatg atgcccgtca
     5701 gtttttcaac agcgattact gtcgtgcatt tgactacttc ccgggtgtat cgggatgatt
     5761 caatcagccg gaagccgtcg agcccgagcg tgccgggaag aaacagcccg atcgcccgcc
     5821 ccatgaacca ctgataggtc agataggtga acgggatttt gagtccctgt cctgccagca
     5881 gcagccgcca tcgaataatg cctgagaaga tgcctgccag ttttacgatc actgcacagc
     5941 tcataaaaaa aagaaaagta gcgggacccg tactgctggc ctctttcagg gcgttccaga
     6001 catccagtgg cgcaacgtct ccgaagagtc ctttcagtcc gaaaagctcc ggctgaaaaa
     6061 taagcaggaa cagggcggcg acaacacaca atttcacagc gattgcaagg ttctttttat
     6121 tcataaacac tctcaaaaag tagattccgg tgtgcggcgg gcgctaaaaa ttccggagtc
     6181 attgtatccg caccggagat tgtttgcaaa caggacgaca cgtcaagtgc ttcagaattc
     6241 tgaaaacaca ggaagaaaaa aacatgccaa ttggcggttt tttacgataa cagattccgg
     6301 ggtgtttctg tttcatgaaa agaggctgac cggaagtacc acacaatgat tcctccgtag
     6361 aaaagcaggc aaagggcagg gaagatcagt gtttcccagc caggattgtc cacattccag
     6421 ggaccaacgc cgtaatccac attcagtgcc ccggctcggc ctttggaaaa aaactgactg
     6481 aaagaagagc ccagcagtcc atggatgaaa cccagtgcca caagattccg gttgcgttct
     6541 tccatgaata catagctaag aaaaatgccc agaagaaaag taaccgcaac cagaccgtat
     6601 gaattaatgt gaatcatgcc gaaacaggat ccggtgagtg tcgcaaccag catccgtttt
     6661 ttgtctttaa agtaaaaccg gagataaagg gtggacagcg gaaaaagaaa aagagtgagc
     6721 ccgggaatta cctgccacgg caggacagta ccatttgagt tcgtaatcac gatatataaa
     6781 agcaggctga aactcagcga ggcaatcaat gctcccgcca cgatctttgt cccggatgca
     6841 gttgcggaac cttcgttttt tgtcggcgca aaggcttttc tcaatcgggt gcccagatag
     6901 gcggaaaaga gaagctgctg tacaaaaccc cagaaaacat agccgaaaac gccggtaaaa
     6961 aacgcattcc aggtgaaatg gtcaaaggca tgtcgccccc gctgtaccag cgcgcccagc
     7021 acaatgaggg caaagagagg cagggcaatt ttcatggcgg ttctgaaagc ggaaaggaaa
     7081 ttgtcataac ggatgcacac gatgacgatg acagaagaga gcagcccgcc gaaaaggaac
     7141 agtccgatga ttccgggaaa ggaggtgttc cagcctgcca tggcagtttt atgcagattg
     7201 aaaaatttta cgacccgcgg ccattgctgg taattgatgt agttgagccc gatgaaaagc
     7261 agcagtatca gcagcacaaa aagtgcccgt tttacagggc tgctctcaga cagaaggcgg
     7321 cgtaatgctc ccggacttcc cagcccccag gaagag
//
LOCUS       DAOPWB010000145         7298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10189_length_7298_cov_5.295734, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000145 DAOPWB010000000
VERSION     DAOPWB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10189_length_7298_cov_5.295734"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1422)
                     /locus_tag="PKN92_11205"
     CDS             complement(37..1422)
                     /locus_tag="PKN92_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49402.1"
                     /translation="MFLRFLPVSFLMLSTVCFPASAGDESFRDLYSDTWAATDGAGRV
                     LPMETEAPPLRKDRFVGIFYWTWHIQYKNGPNDNTKLIQAAGDSPEVEWPVNGTPHHW
                     GEPELGYYWMTDPFVIRKHASMLTDAGVDALFFDTTNAPFTWKEEYEALCREYERMRQ
                     EGNQTPAICFIAPFGDPMPVLERVWEDLYKPGLWEDLWFRWEGKPLILANPDYVKDPE
                     MRAFFTFRRPMPDYWLGPSGPDQWSWLEVYPQHEFKNSRGETEQMSVGVAQNALPNTP
                     GPAPMSHTRGAMGRSWHNGGKDPSPDAVKLGLNFDEQWRYALEKDPTFIFVTGWNEWI
                     AGRFQKWSIYTDETSYYPGGLFVDQYTQEYSRDCEPMRGGHEDNYYYQLAHWIRKYKG
                     VRPLPRIPGPGNIRIDGIFNDWSDIQPEYRDARGDITHRDHKGFGEIHYTNTTGRNDF
                     ITAKASWRIQG"
     gene            complement(1462..2733)
                     /locus_tag="PKN92_11210"
     CDS             complement(1462..2733)
                     /locus_tag="PKN92_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNZ49403.1"
                     /translation="MNPSVSALVIFIVAYSLFVIFPMRRSFSALGAVALLFITQTIDP
                     VDALVHGVNWNVMGIFVGMLVLADVFMGSRFPAYLAEIIVNRAPNTAVAILFICVMTS
                     VISAFVENVATVLIVAPVGLALAKKLDFNPINMMTAIAISSNLQGCATLIGDPPSMLL
                     GGFAKMNFMDFFWYQGKPGIFFAVQIGAVTSSLYLYFVFREHKVKTKLLPEEKVLSWI
                     PTLLLLALIMALAGTSFFDKEFTYLAGLICMLFAVAAIIWDRATNQEEFWINMKKLDW
                     DTTFFLFGVFIIVYSLTETGWIDQISAFLSARIGGNVLLGYLLLLIVSIIVSGFVDNV
                     PFLAAMLPVALDMSHKLNINPAIFLFGLLLGASLGGNITPIGASANIVACGMLKKDGH
                     PITFGQFMKVSVPFTIAAVVPAAIFVWFMWA"
     gene            complement(2845..4860)
                     /locus_tag="PKN92_11215"
     CDS             complement(2845..4860)
                     /locus_tag="PKN92_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNZ49404.1"
                     /translation="MGTLNAIDYSVIGAYLAALVVLGFALEKMASQSLDDYFIGGRRL
                     PWWALGISGMAYFLDMSGTMIITSFLFLLGPRGLFIEFRGGAVLVLAFMMLWGGKWHR
                     RSKCLTSAEWMVFRFGDGWSGRFAQAVSALAGVVSAVGMLAYLIKGAGLFLTLFFPYS
                     PLTCTLIMIGVTTFYTMISGFYGVVFTDLFQAAIILIGVILVTVMAVLRLQGTADFSL
                     IASQVTGNAQWLESLPRWHTTMPAGYEQYEALIMFAFFYLMKNIFLGIGTGADPRYLG
                     AKSDRECGLLSLFWTSLMTIRWPMMISFAVLGIFLVHDIFPDQTALSTTAALIKSETG
                     VADKTVWADTLSHIANHPDAYSEEFIQQLKGMLGNGQWQKKLLLLGYEGHVDPERILP
                     AVLLFTIPPGLRGLLLVVLLAAAMSTFNATINLTTGLITRDFYQKYLRPRASTRELIY
                     TNWAVVLLLVVISFLFAFSIQSINDIWAWIIMGLGAGLMIPGILRLLWWRFNGGGFAA
                     GTFAGLLAAVLQRWFYPGLDERLQLVFLGLIGFLAALAGTWLTPPEEDSVVARFYNIT
                     RPFGLWGPYKKRLAEKDLLELDVEHRRDLLTCPFALLWQICMFLAPMQFVVGAWRGFC
                     VTSILWLLCGLVMYFVWFRHLPAENYFDPCEEAGVCLGEGEQGRLTK"
     gene            complement(5032..7062)
                     /gene="fusA"
                     /locus_tag="PKN92_11220"
     CDS             complement(5032..7062)
                     /gene="fusA"
                     /locus_tag="PKN92_11220"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNZ49405.1"
                     /translation="MATIEAGKIRNVGIVGHGGVGKTTLVEHILHMAGLTGRLGTVEE
                     GNTVCDYLEEEIERKHSIAMALANTEWKGGRIHLIDHPGYMDFLGEMASSTPLVDGLV
                     IVVDASTGVQVGMDNAWKYAERFKVPRAFFVNKLDREHTSFDETVHLLQETYGRHCVP
                     IVIPVGEGADLKDVVNIVTGDNLPAGADALKEALVEAVAETDDTLMEKYLETLELSPE
                     EFRNGLQKGITSGQIIPILAGSVALEKGITQLLDMIAESFPSPLDRTINALDEKGEEV
                     TVTPDPNGPFLAQVFRSIVDPFVGHLTLFRVLSGTLKSDTDFYNVTSNTKERSGKILL
                     LNGKQQTPIDAVLPGDLAAMTKLKNTHFGDTIGALNCNLHMAPIQLPESMVKLAIQPK
                     ARSDEDKIGEALNRLAEEDPTFTHYRDTATNEHVIRGMGDLQLDILLSRMQRKYKVEA
                     ETRIPKVAYLETIKSKVEVQGKHKKQSGGHGQYGDVHLRISPNERGAGYQFIDSIVGG
                     VVPKQYIPHVDKGGFEALERGVISGHPVVDVIVELFFGSYHAVDSSEMAFKLAASKAI
                     QKGIREARPCLLEPIMEIAVTVPEEFMGDINGDLNSRRGRILGMDALGGGRQVIRANV
                     PEAEILRYSTELRSMTQGRGNYEVHLSHYDEVPDHIAKEIIAAYEKTRTEEE"
BASE COUNT         1950 a         1839 c         1842 g         1667 t
ORIGIN      
        1 gcctgccgaa gtcttattca attcaataat tgcatttcag ccttgaatcc gccaagaggc
       61 tttcgccgta atgaagtcat ttctgccggt agtattggtg tagtgtattt caccaaaacc
      121 tttgtgatcc ctgtgggtaa tatcgccccg ggcatcccgg tactcgggct gtatatccga
      181 ccagtcattg aatatgccgt ctatccggat attaccgggt ccgggtatgc ggggaagagg
      241 gcgaacccct ttgtattttc ggatccagtg tgccagctga taataataat tgtcctcgtg
      301 cccgccacgc atgggctcgc agtcacggct gtattcctgt gtatactggt ctacaaaaag
      361 cccgccggga tagtagctgg tttcatccgt atagatgctc catttttgaa agcgccccgc
      421 tatccattcg ttccacccgg ttacaaaaat gaaggtcgga tctttttcga gggcgtaccg
      481 ccactgctca tcaaaattaa gccccagttt gaccgcatcg ggcgacgggt ctttgcctcc
      541 gttatgccag cttctgccca tggcacctct tgtgtggctc attggtgccg gacccggcgt
      601 gttggggagt gcattctgag caacgcctac gctcatctgc tctgtttcgc cgcggctgtt
      661 tttgaactca tgctgcggat aaacttcaag ccagctccat tgatcgggac cactcgggcc
      721 tagccagtaa tcgggcatag gccggcggaa agtaaagaat gcacgcattt ccggatcttt
      781 cacatagtcc ggattggcaa gaatcagagg ttttccttcc caacggaacc aaagatcctc
      841 ccagagcccg ggtttataca gatcctccca gacgcgctcc agaacaggca tgggatcgcc
      901 aaagggtgct ataaaacaaa tggcaggtgt ttggttgcct tcctgtcgca tgcgctcata
      961 ttccctgcat agcgcctcat attcttcttt ccaggtgaag ggggcattgg tcgtatcaaa
     1021 aaagagagca tctacgcccg catcggtgag catactggca tgtttcctta tgacaaaggg
     1081 atcggtcatc cagtaataac ccagttccgg ctcaccccag tgatggggcg ttccgttgac
     1141 aggccattcc acttccggac tgtcgcctgc cgcctgtatc agtttggtgt tgtcattagg
     1201 tccgttttta tactggatgt gccaggtcca gtaaaaaatg ccgacaaaac ggtcttttct
     1261 gagcggaggg gcttcggttt ccatgggaag gacacgaccg gcaccatctg ttgctgccca
     1321 ggtatctgaa tagagatccc ggaaagattc gtccccggct gaggccggaa agcagactgt
     1381 gctcaacatt agaaatgaaa ccggcagaaa ccgtaagaac atggcggaat ccttcgcagc
     1441 taatgatcgg caatacaagg actatgccca cataaaccaa acgaaaatcg cggcgggtac
     1501 cacagcggcg atggtgaagg gaacagatac tttcataaat tgcccgaaag taatgggatg
     1561 cccgtctttt ttaagcatgc cgcaggcaac tatatttgcg gatgcaccaa tgggcgtgat
     1621 gtttccgccc aggctggccc cgagaaggag accgaaaaga aaaatggctg gattgatgtt
     1681 taatttatgg gacatgtcta aagctacggg aagcatagcg gcaagaaagg gcacattgtc
     1741 cacaaagccc gaaacaataa tggacacaat cagaagaagc agataaccga gcaacacatt
     1801 gccgccgatc cgggccgaaa gaaatgcgga gatctgatct atccagcctg tctcggtgag
     1861 gctgtacacg attatgaaca cgccaaacag aaagaaggtg gtgtcccaat cgagcttctt
     1921 catgttgatc cagaactctt cctgattggt ggcacgatcc cagatgatgg cagccacagc
     1981 aaaaagcata cagatcagcc cggcaagata cgtaaactcc ttgtcaaaaa aggaagtgcc
     2041 tgccaacgcc ataatcaatg cgagcagcag aagcgtagga atccaggaaa gtactttttc
     2101 ttcaggcaaa agttttgttt ttactttatg ttctctgaaa acgaagtaca ggtaaaggga
     2161 ggaagtgaca gcacctatct ggacggcaaa aaagatccca ggctttccct gataccagaa
     2221 aaaatccata aaattcattt ttgcaaagcc gccgagcagc atgctgggcg ggtcgccgat
     2281 gagggtggca cagccctgaa gattgctcga gatagcaatg gcagtcatca tattgattgg
     2341 attgaaatcc agttttttcg ccaaagcaag accgacaggt gcaactataa ggacggtagc
     2401 cacattttcc acaaaagcgg aaatcacact tgtcatcacg cagataaaaa gaatggcaac
     2461 agccgtgtta ggagcgcggt tcacgatgat ttcagcgaga taagcaggga atcggctgcc
     2521 cataaaaaca tctgccagca caagcatacc cacaaatatg cccatgacat tccagttaac
     2581 tccatggacc agagcgtcta cggggtctat agtctgcgta ataaaaagga gcgctacagc
     2641 gcccagagcc gaaaaacttc ggcgcatggg aaaaatcaca aaaaggctgt acgccacaat
     2701 aaaaataaca agagcagaaa cggaggggtt cattgtagac tctttctgaa tgaagcgggt
     2761 agagtcagta gtttaacgca tttatgcggg agagcgcaaa gatcattgcg aagacaaaac
     2821 acatgggcgc ttctggctgt tgtgctattt cgtcagccgt ccctgttctc cttctccaag
     2881 acatacgccc gcttcttcgc agggatcaaa atagttctca gcgggaaggt gcctgaacca
     2941 cacaaaatac atgaccagcc cgcagagaag ccacaaaatt gaggtgacac agaagccccg
     3001 ccaggcgccg accacaaact gcataggcgc aaggaacata cagatttgcc atagcagcgc
     3061 aaaaggacag gtgagtaagt cgcgtctgtg ctccacatcc agttccagaa gatctttttc
     3121 agccagcctc tttttataag ggccccacag tccgaagggg cgggttatat tataaaaacg
     3181 agctacaacg gaatcctctt caggaggcgt aagccaggtg cctgccaggg cggcaaggaa
     3241 cccgataagc cctaaaaaaa ctaactgaag ccgttcatcc agccccggat aaaaccagcg
     3301 ctgcagtact gccgccagta gacccgcgaa ggttcccgca gcaaagcctc cgccattaaa
     3361 ccgccaccag agcaggcgca gaatacccgg gatcataagc ccggcaccca gacccatgat
     3421 gatccacgcc cagatgtcgt tgatgctctg gatggaaaaa gcgaaaagaa aagagattac
     3481 aaccagaagc aggacaacgg cccagttggt atagatcaat tcacgggtgg aggcgcgggg
     3541 ccttagatat ttctggtaga aatctcgtgt aatcaggcct gtcgtaagat tgatggtggc
     3601 attgaacgtg gacattgccg ctgccaggag caccaccaga agcaggccgc gtaatccggg
     3661 cgggatagta aagagcaaca ccgccggaag aatgcgctca ggatcaacgt gcccttcata
     3721 cccgagcaga agcagttttt tttgccactg gccattgccg agcataccct tcaactgttg
     3781 tataaattct tcagagtagg catcggggtg gttggcaata tgggataagg tgtcggccca
     3841 tacggttttg tccgctacac ctgtttcact ttttatgagt gcagcagtgg ttgaaagtgc
     3901 ggtttgatca ggaaaaatat catggacaag gaagataccg agaacggcaa aggagatcat
     3961 catgggccag cgaatggtca taagtgaagt ccagaaaagg cttagcagac cgcattccct
     4021 gtcgcttttt gctcccagat aacggggatc tgcacccgta cctataccta ggaaaatgtt
     4081 tttcatcagg taaaaaaaag cgaacatgat caatgcttca tattgttcat atccggcggg
     4141 cattgttgta tgccaacgcg gcaggctttc aagccattgc gcattgcctg ttacctgaga
     4201 tgcaattaaa ctgaaatctg ccgttccctg aagccgaaga acagccatga ccgtaacaag
     4261 aataacgccg atcaggataa tggcggcttg aaacagatca gtaaagacga ccccgtaaaa
     4321 cccggaaatc atggtgtaga aggtagtgac gccgatcatg atcagcgtac aggtcaaagg
     4381 cgaatagggg aaaaagagtg taagaaaaag acctgccccc ttgatcagat aggcaagcat
     4441 cccgacggcg ctgacaacgc ccgcaagtgc cgaaacagcc tgtgcaaaac gcccgctcca
     4501 gccgtcaccg aagcgaaaaa ccatccattc cgccgaggtc agacacttgg agcggcgatg
     4561 ccacttgccg ccccatagca tcatgaatgc cagcaccagg acagctccgc ctctgaattc
     4621 gataaaaaga ccgcgcggtc ccagcaggaa gagaaaagag gtgatgatca tggtaccaga
     4681 catgtccaga aaataagcca tgccggaaat gcctagcgcc caccacggca gacgtcgtcc
     4741 tccaataaag tagtcatcaa gactttggga tgccatcttt tccagcgcaa agcccaacac
     4801 gaccaaagca gcgagataag cgccgattac agaatagtct atggcgttga gtgtacccat
     4861 caccctttcc tttccaagtt ctctttctgc aagcgaagag aacttaacag ggaagacaga
     4921 accaaaaggg tatagttaca ggaaaacgga gtctaattca actgaaatga aaaagaaaaa
     4981 cggcctgcgc aaggcagacc gttaaaaagc gggggtagga agtaagaaag gttattcttc
     5041 ttctgtcctg gtcttttcgt aagcggcgat aatttccttg gcaatatgat ccggcacttc
     5101 gtcatagtgg ctgaggtgta cttcgtaatt gcctctgccc tgcgtcatgc ttcggagttc
     5161 ggtggagtaa cgaaggattt ccgcttcagg gacgtttgca cggatcacct gcctgccacc
     5221 gcccagagcg tccatgccga ggattcggcc gcgtcggctg ttcagatcgc cgttgatgtc
     5281 acccatgaat tcttccggta cagtgacggc aatttccata atgggttcga gcaggcaggg
     5341 gcgtgcttcg cgaataccct tttggatagc tttggaagcc gccagtttaa atgccatttc
     5401 cgaagagtcc acagcatggt aggagccgaa gaaaagttcc acaatgacgt ccacaaccgg
     5461 atgtccgctg ataacgccac gttccagcgc ttcaaagccg cctttgtcaa cgtgcgggat
     5521 atactgcttg ggtactacgc cgccgacaat actgtcaata aactggtatc cggcaccgcg
     5581 ttcattcgga gagatacgaa ggtgcacatc gccgtactga ccgtggccgc cactctgctt
     5641 tttgtgtttg ccctgcacct caactttgct tttaatggtc tccagatagg caactttggg
     5701 tatacgggtt tctgcttcca ctttatactt gcgctgcatg cggctaagaa gaatatccag
     5761 ctgaagatcg cccattcctc tgatcacatg ctcgttggtt gccgtgtcac gatagtgggt
     5821 aaaggttgga tcttcttctg ccagcctgtt cagggcttca ccgattttgt cttcgtcgga
     5881 gcgggctttc ggctggattg ccagttttac catcgattca ggcagctgaa ttggcgccat
     5941 atgaagattg cagtttaatg cgccgatggt atcgccgaag tgcgtgtttt taagtttggt
     6001 catggcggca aggtcgccgg gaagcactgc gtcgatgggg gtctgctgtt taccgttcaa
     6061 aagaagaatt ttgccggaac gttcttttgt attggacgta acgttgtaaa aatctgtgtc
     6121 ggatttcagt gtgccgctca gtacccggaa aagcgtcaga tggccgacaa aggggtcaac
     6181 gatggaacgg aaaacctgcg caagaaaagg cccgttagga tcgggtgtaa ccgtcacttc
     6241 ttcgcctttt tcatccagag cgttgatggt acggtcaagg ggactgggga aagattcagc
     6301 gatcatgtcc agaagctggg taattccttt ttccagagca acgctgcctg ccagaatggg
     6361 aataatctgt ccggaggtaa tccctttctg aagaccattg cggaattctt ccggtgaaag
     6421 ttccagcgtc tccagatatt tttccatcaa ggtatcgtct gtttcggcaa cagcctcaac
     6481 aagcgcttct ttcagcgcgt cggcaccggc aggaagattg tcgccggtaa caatgttgac
     6541 aacatccttc agatcagccc cttcgccgac aggaatcacg ataggaacac agtggcgacc
     6601 gtaggtctcc tgcagcagat gtactgtttc atcaaaactt gtgtgttcgc gatccagttt
     6661 gtttacgaaa aaagcacggg gcactttaaa gcgttctgcg tatttccagg cattgtccat
     6721 accaacctga acgcctgtag aagcatcgac tacgatcacc agcccgtcaa ccagcggagt
     6781 ggatgaagcc atttccccca gaaagtccat gtatcccgga tgatcaatca gatgaatccg
     6841 gccgcctttc cattccgtat tggcaagcgc catggctata ctgtgcttcc gttctatctc
     6901 ctcttcgaga tagtcgcaaa cagtgttgcc ttcttctact gttcccagac ggccggtaag
     6961 acccgccatg tggagaatgt gctcgacgag cgtcgttttt ccgacgccgc cgtgacctac
     7021 aatgccaaca ttgcgaatct ttccagcttc aattgtcgcc atgaatcaaa ccctccggca
     7081 cagattttgc ctgatgtaca ggcgttatgg tttcagtcaa aaataaacat aagggtattg
     7141 cagataatac aatcaggggt ctgtagtata ccctttttcc cttcaaggca tcaaatttat
     7201 actgaaaacg ttcctgaact gctttggcag gcaataaaag ccgaggcgga ttcaaggctg
     7261 aaatgcaatt attgaattga ataagacttc ggcaggcg
//
LOCUS       DAOPWB010000146         7178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10427_length_7178_cov_5.605644, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000146 DAOPWB010000000
VERSION     DAOPWB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10427_length_7178_cov_5.605644"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1043)
                     /locus_tag="PKN92_11225"
     CDS             complement(81..1043)
                     /locus_tag="PKN92_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49406.1"
                     /translation="MKRTALIVLQFLLMSLTAVAQDNAPTPPSEVSFMLPPSATAGTP
                     PQPPAATSGHASSPSGTGAVPPGTAPVNAVPSPGMMVQPQTPPVVSGRLYAVHASGGI
                     PQKLKIDKRLKSLKPLFSSLPFTSYEAVAVHDKELPWGEETLFPVNAVYTLKVMPLSM
                     SQEGAVALQARVEMLQDSTYVNALNTLAEAHMNQALLFRGLPAGADELLLVFIVTMPS
                     DDQNQQDQQDQQDQQDQQDQDQDQDQDQEGETSDAEDPAAREGEEESPETADLSDAEE
                     NEETKEENNENLEALLQSLEETDRREQVEERNKRRSVEIKGNWW"
     gene            complement(1040..1744)
                     /locus_tag="PKN92_11230"
     CDS             complement(1040..1744)
                     /locus_tag="PKN92_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49407.1"
                     /translation="MADKRYQISGVLCVVFLLMTGIAQAAGLQDAIREAMNHLDTGDA
                     DGALAQLQELQVEHPLSSELRFALGYAWAVQAQRLAQSGDAEKQKSAVAEALAFFDGL
                     LYDPQKNIAQEAAFNRATLLAQEAMRLDPAADFKAAVAALQRAVAAFEEAAKRYPDSS
                     KIYRNLEHLRLKLKELLKQQPDEEQQEEEKPEKEEQPPILSRFGQASTDIPGARAEMT
                     DNTVQLITPEAKEGES"
     gene            complement(1737..2810)
                     /locus_tag="PKN92_11235"
     CDS             complement(1737..2810)
                     /locus_tag="PKN92_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ49408.1"
                     /translation="MQFSFPLHQWPLWTALAVLILAVCAVLLYMLEKRRGARLNRFVE
                     SGLAAQLMPGWSPERRRPLAWIFLLGILFVLLAEAGPRWGKKWAPVTRVSRDVLVVLD
                     VSLSMDAENPPPTRLVRARQKIESLLERSPADRFGLVAFSGEAATLCPLTLDHGYFRS
                     VLDAINTNTLSTDGSDISAALKEALDVFQEDAQRFGEDNSGDRVVLLLSDGEETAGDA
                     LALAEQIGRYARIYVIGIGDPQGAVVTYPAWLRQYVRIPEDKLTRLSKLDEENLSRIA
                     VQSGGAYVRITPDNSDVDFIQQELKQLRSRVTAGEVRFQMTERYQWPLILAWFFFFLE
                     GVWMLMLAPMRKRRMRLEAQNHG"
     gene            complement(2880..3914)
                     /locus_tag="PKN92_11240"
     CDS             complement(2880..3914)
                     /locus_tag="PKN92_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ49409.1"
                     /translation="MNWLSRLLASPGFSSPEMLWLLLPVLALFIWECFPAASGVIHIS
                     TGERIRSLYRYHSGVLSRLTALGRALGLALLTVALAGPLEGWQLNKERAGIIDIVLCL
                     DVSGSMRETDFLVGGRPRDRMYVAREAARSFIDSRRETAGSRYGLDRVGLVLFSGLAW
                     TQCPLTLDYDVLEHELDRAEIADERKQGTAIGSALGLAVRRLSKSEAKSKVVILLTDG
                     QNNRGDLDPMTAARLAKEYGIRVYTIGAGSEEEKIVGQGFFSRRTEAIDGKMLSGIAS
                     MTGGKYYLASDTRSLMNAYDEISEMEATEIEIGDYKEAGVMYLPFLTAGALVLVFSGL
                     ARRHWFEQIP"
     gene            complement(3911..4852)
                     /locus_tag="PKN92_11245"
     CDS             complement(3911..4852)
                     /locus_tag="PKN92_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49410.1"
                     /translation="MMRSVRILILSFLAVLPAASDFPIDALLEPPEAPWHRSIAVELS
                     VQAPASAQITFPDVAEKKGQVQVRKSEKNVEHLDDGTLRHRQQYVIDPLSAGNWLFPP
                     LNIGWADGENTGVLESAPLAFTARELTPEEKEFASHFEGLTSPSFFLKERRNYRWLYL
                     FPVLAAAAVCVLLALWWKRRRAYQPAPPPLPPWTMALNRLRELQTRDLPGQGRSDLYY
                     VDLSAIMRYYFEDRFQIPAPEQTTPELLEAAQKETELRDDQHLFLKSFLCQCDRVKFA
                     RYNPGIAEMEEHFTGVKTLVQQTIPDTVFRTEGDDRL"
     gene            complement(4849..5742)
                     /locus_tag="PKN92_11250"
     CDS             complement(4849..5742)
                     /locus_tag="PKN92_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNZ49411.1"
                     /translation="MISQDAIKKIRRIQIRTRRTVNEILAGQYQSAFKGQGMEFREVC
                     SYVPGDDIRSIDWNVTARTGEPHVKVMAEERELTVALLVDMSGSGRFGSVSRLKNELA
                     AELCAVLALSAINNNDKVSLLIFTDDLELYVPPQKGSRHVLRIIREVLYFQPKGRATN
                     ITRALNYLNRVISRKAVVFLVSDFMDQGYEDALRVTAKRHDVIAVSVSDPREMSLPDV
                     GRVAVADAETGREILINTGDRKLREAYAQKAREETAAREEIFRRNRIDSLRLSTDKSY
                     VDELYRFFKLRERRRAGRAAL"
     gene            complement(5767..6222)
                     /locus_tag="PKN92_11255"
     CDS             complement(5767..6222)
                     /locus_tag="PKN92_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49412.1"
                     /translation="MAAKKKEFSSKALIALAEATGADIAAQAEAPPDRNTLFALLEKA
                     WSKAGVPSKSDVWFPEYLDGMKLNCGFAVDFLLDKRLAVMIASPEKPKSVVEEELGFC
                     TDNHNFEACVMLNFGKKFRCIPFEYNTITGSWLRVPEQSQNILREQGKK"
     gene            complement(6261..>7178)
                     /locus_tag="PKN92_11260"
     CDS             complement(6261..>7178)
                     /locus_tag="PKN92_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNZ49413.1"
                     /translation="EEIGRIIVGQQYMIDRLLTAMLANGHVLIEGVPGLAKTTAVTTL
                     AHAMDCSFRRIQFTPDLLPADLIGTLIYTPKTGEFVTRKGPVFGNIILADEINRAPAK
                     VQSALLEAMQERQVTIGENTYALDEPFMVLATQNPIEQEGTYPLPEAQVDRFMLKLRV
                     TYPSRQEEREIMDRVDLLHPAGVSTVVTKEQLLQAREVVNRIYVDDKAKNYIVDLVYA
                     TREPEAFDIPIRNLIEYGASPRASIYLQQASRAMAFLQGEGNVFPNDVKQVAMDVLRH
                     RIKVSYEAEAENITSEDIIRRILENVPVP"
BASE COUNT         1659 a         2065 c         1777 g         1677 t
ORIGIN      
        1 cgccgaaatg gatacgcccc cctgcgccac agcaattcgt gcggccaaca aaaagataag
       61 actgtaacag aacagacggt tcaccaccag tttcctttga tttcaacact tcgccgctta
      121 ttgcgttctt ccacttgctc ccggcgatcg gtttcttcca gagactgaag caaagcctcc
      181 agattttcat tattttcctc ttttgtttcc tcattctctt cggcatcgga aagatcagct
      241 gtctcaggag attcttcttc tccttctcgc gcagccggat cctcggcatc cgatgtttca
      301 ccttcctgat cctgatcctg atcctgatcc tgatcctgtt gatcctgctg atcctgttga
      361 tcctgttgat cctgttgatt ctgatcatcg gagggcatcg tcactataaa gacaagaagc
      421 agttcatctg ctcctgcagg cagccctcta aaaagcagcg cctggttcat gtgtgcttcc
      481 gccagcgtat tcagggcatt tacataagtg ctatcctgaa gcatttccac acgcgcctgc
      541 agagcaaccg ccccttcctg gctcatgctc aacggcatga ctttcaatgt ataaacggca
      601 ttgacaggaa agagtgtttc ctctccccag ggcaattctt tatcgtgcac agcgactgct
      661 tcatagcttg tgaaaggcag agaggaaaac agcggtttta aagacttgag tcttttatca
      721 atttttaatt tctgcgggat accgccggag gcatggacgg cataaagcct gcctgaaaca
      781 acgggcggtg tctggggctg caccatcata cccggagaag gcaccgcatt caccggtgcc
      841 gtgccaggtg gcacggctcc agtcccagaa ggtgaagaag catgccctga tgtggctgct
      901 ggtggctgag gcggcgtgcc tgccgtggcg gagggaggca gcataaaaga cacctccgac
      961 ggcggcgtag gggcgttgtc ctgtgccacg gctgtcaacg acatcagcaa gaactggaga
     1021 acgatcaagg cagtgcgttt catgattcgc cctcctttgc ttcgggcgtt atcagttgta
     1081 ctgtgttgtc tgtcatttcc gcacgggcac cgggaatatc agtggatgcc tgtccgaaac
     1141 gggacagaat cggcggctgc tcttctttct ccggcttttc ttcctcttgt tgctcttcgt
     1201 ccggctgctg cttaagcaat tctttcagct tgaggcgcag atgctccaga ttcctgtaaa
     1261 ttttagaaga atcgggataa cgtttcgccg cttcttcgaa agctgcgaca gcacgctgca
     1321 gggcagccac agcggcctta aagtctgcgg cgggatcaag gcgcattgct tcctgtgcca
     1381 gcagggtcgc acggttaaaa gcagcttcct gcgcaatatt tttttgcgga tcataaagca
     1441 gaccgtcaaa aaaagcgagc gcttccgcca ccgcactttt ttgcttttcc gcatcaccgc
     1501 tttgtgcgag tctctgcgcc tgcactgccc aggcgtatcc aagcgcaaag cgaagttccg
     1561 atgacagcgg gtgttccacc tgcagttcct gcagctgtgc cagcgctccg tctgcgtctc
     1621 ccgtgtcaag atggttcatc gcttcccgga tggcatcctg cagccccgcc gcctgagcaa
     1681 tgccggtcat caaaaggaaa acgacacaga ggacgcccga aatctgatac cgtttgtcag
     1741 ccatgattct gtgcctccag acgcatgcgc cgttttcgca tgggtgccag catcagcatc
     1801 cagacacctt ccaggaaaaa gaaaaaccag gcaagtatca aaggccactg atacctctcc
     1861 gtcatttgaa agcgcacttc tcctgctgta acccggcttc tcagctgctt cagctcctgc
     1921 tggataaagt caacgtcgct gttgtccggc gttatccgga cataggcgcc gccgctttgc
     1981 acagcaatcc tggacaggtt ctcttcatcc agttttgaaa gacgggtgag tttatcttcg
     2041 ggaatacgca catactgacg cagccaggcg ggataggtaa cgacggctcc ctgcggatct
     2101 ccaataccaa taacataaat acgcgcatag cgtccgattt gctctgccag cgccagggca
     2161 tcccctgctg tttcttcgcc atcactgagc agcagcacca cgcggtcgcc gctattgtct
     2221 tcgccaaagc gctgcgcatc ttcctgaaag acatcgagcg cctctttcaa tgcagcactg
     2281 atatctgatc cgtccgtgct gagcgtgttg gtattaattg catccagcac agaccggaaa
     2341 taaccatgat ccagcgtgag gggacacagc gtagccgcct caccggaaaa agccaccaga
     2401 ccgaaacggt cagccggcga acgctccagc agtgattcta ttttctggcg tgcacgaacc
     2461 agacgcgtcg gcggcggatt ttccgcatcc atgctaagcg aaacatccag cactaccagc
     2521 acatcccggc ttacccgggt aacaggagcc cattttttgc cccagcgcgg tccggcctcc
     2581 gccagaagca caaaaagtat gcccagcaga aaaatccagg cgagcggcct gcgccgctca
     2641 ggcgaccagc cgggcatcaa ttgcgcagca agtccggact caacgaagcg gttaagccgt
     2701 gcaccccgtc ttttttccag catgtacagc agcacagcac acactgccag aataagaacg
     2761 gccagtgctg tccaaagcgg ccattgatgg agcggaaaag agaattgcat atacttaccg
     2821 cttccgtttc cgcccgggtg aatatttttt tatttcagca aaaaaccaca tagtgtgcct
     2881 catggaattt gttcgaacca atggcgccgc gccagtcctg aaaaaaccag caccagtgcg
     2941 cctgccgtca gaaaaggcaa atacatcaca cctgcttctt tgtagtcacc gatctcgatt
     3001 tccgttgctt ccatttcgct gatctcatca taggcattca tgagagagcg tgtatccgag
     3061 gcaagataat attttcctcc ggtcatggaa gcaatcccgc tcagcatttt tccgtcgatg
     3121 gcttctgtcc gccgcgagaa aaagccctgc ccgacaattt tttcttcttc tgatccggca
     3181 ccgatggtat aaacgcgtat cccgtattct ttggcgagcc gcgccgccgt cataggatca
     3241 agatcgcccc ggttgttctg gccgtccgtc agcagaatga cgacctttga ttttgcctct
     3301 gatttgctga gacggcgcac tgccaggccc aatgctgaac cgatggcagt accctgcttc
     3361 cgttcgtctg ctatctcagc gcgatccagt tcgtgctcca gaacatcata atcaagcgtc
     3421 aggggacact gtgtccacgc cagtcctgaa aaaagaacca gccccacacg atccagaccg
     3481 tagcgtgagc ccgccgtttc ccggcgactg tcaataaagc tccgtgcggc ttcccgggca
     3541 acatacatac gatcccgcgg gcgtccgccg acgagaaagt ccgtttcacg catactgcct
     3601 gacacgtcca gacataaaac aatgtcaata atgccggcac gttctttatt caactgccag
     3661 ccttccaacg ggcccgccag cgcaacagtc agcaaggcaa gtccaagggc acgccccaaa
     3721 gctgtcagcc gggaaagaac gcctgagtga taccggtaaa gcgaacggat ccgctctcct
     3781 gtggagatat gaatcacgcc ggaggcggcc gggaaacatt cccaaatgaa caacgccagc
     3841 acaggcagaa gcagccagag catttccggt gatgaaaatc cgggtgaagc cagaagtcgg
     3901 gacagccagt tcataagcgg tcatcccctt ctgttcggaa tactgtatcc ggaatggtct
     3961 gctgtacaag cgttttaacg ccggtgaaat gctcctccat ttccgcaatg ccggggttat
     4021 accgggcaaa cttgacccga tcacattgac agagaaagga cttgagaaac agatgctggt
     4081 cgtctcggag ttccgtttct ttctgagccg cttccagcag ttccggcgtg gtctgctcgg
     4141 gagcaggaat ctgaaaccgg tcttcaaagt aataccgcat aatggcactg agatcgacgt
     4201 aataaagatc cgatcgcccc tgccccggca gatctctagt ctgcagctca cggagccggt
     4261 tcagtgccat cgtccacggc ggcaacggag gcggcgcggg ctgatatgcc cgacggcgct
     4321 tccaccacaa tgcgagcagg acacagaccg ctgctgcagc cagcaccgga aacaaataca
     4381 gccatctgta attccgtctt tctttcagga aaaacgacgg cgaagtaagc ccttcaaaat
     4441 gcgaggcgaa ctccttttct tcgggagtca attctcgggc ggtaaatgca agcggtgcgc
     4501 tttccagaac acctgtgttt tctccgtccg cccagccaat attcagaggc gggaaaagcc
     4561 agttgcccgc agacaaggga tcaatcacat actgctgccg gtgccggagc gtgccatcat
     4621 ccaaatgctc cacgtttttt tcagacttcc gaacctgaac ctgtcctttt ttttcagcaa
     4681 catcaggaaa agtaatctgc gccgatgccg gtgcctgcac agacaactcc acggcaatgg
     4741 agcgatgcca gggggcttca ggcggttcaa gcagcgcatc gatgggaaaa tccgacgcgg
     4801 caggcagtac cgccagaaaa gaaaggatca gaatccggac acttcgcatc acagggctgc
     4861 cctccctgcc cggcgacgtt cacggagttt aaaaaagcgg tacagctcat ccacatagga
     4921 tttgtccgtt gaaaggcgga ggctgtcaat ccggttacgc cggaaaattt cttcccgtgc
     4981 cgcagtctct tcccgcgctt tttgcgcata ggcttccctg agttttctgt cgcctgtatt
     5041 gatcagtatt tctctccctg tttcagcatc agcaacagcg acccgcccca catcaggcaa
     5101 actcatttct ctgggatcgg aaacagagac cgcaataacg tcatgccgtt tcgctgtcac
     5161 ccgcaaagca tcttcataac cctgatccat gaaatctgaa acaagaaaga ccacagcttt
     5221 tctggagatg accctgttca gataattcag agcacgcgta atattcgttg ccctgccttt
     5281 gggctgaaag taaaggactt ccctaatgat acgcagtaca tggcggctcc ctttctgcgg
     5341 cgggacataa agctccagat catcggtaaa gatcagaagg ctgactttat cgttattgtt
     5401 gatcgctgaa agcgccagca cggcacaaag ttccgctgcg agctcgtttt tcagacggga
     5461 cacgcttcca aaccggccgg aaccgctcat atccaccaaa agcgccaccg tcagctcccg
     5521 ttcttccgcc attactttca catgaggctc gcccgttcgc gctgtgacat tccagtcaat
     5581 cgagcggata tcgtcgcccg gcacatagga acacacttca cggaattcca tgccctgccc
     5641 tttgaaggcg ctctgatact gccccgccag aatctcatta actgtccggc gggtgcggat
     5701 ctgaatgcgc cttatttttt tgatggcatc ctgtgaaatc atggaatcct gtctcctgcc
     5761 cggagtctat tttttgcctt gttctcgaag aatattctgg ctctgttcag gaactctcag
     5821 ccagcttccg gtaattgtgt tgtactcgaa agggatgcat cggaattttt ttccgaagtt
     5881 caacattacg caggcttcaa aattgtggtt gtctgtgcaa aaaccgagtt cctcttcaac
     5941 aacggatttc ggtttttccg gactggcaat catgacggca agccgcttat ccagaagaaa
     6001 gtcgacagca aagccacagt tcagcttcat gccgtccaga tattcgggaa accagacatc
     6061 ggatttggag ggaacccctg ctttgctcca ggctttttca agcaatgcaa agagggtgtt
     6121 ccgatccggc ggcgcttcag cctgtgcggc aatatccgca cccgttgctt cggcaagggc
     6181 aatcagcgcc ttggaactga attctttctt ttttgctgcc ataactgcgg tactttcatt
     6241 tttacggtag ttcggaatca tcagggcaca ggcacatttt caagaatccg gcggataata
     6301 tcttcgctgg taatattttc cgcttccgcc tcgtaactca ctttgatgcg gtgcctgagt
     6361 acatccatgg caacctgttt gacatcattg ggaaagacat tcccttcgcc ctgaagaaat
     6421 gccatggcgc gggatgcctg ctgcagataa atggacgccc ggggagaggc accgtattca
     6481 atgagattcc ggatgggaat atcaaaggcc tcaggctccc gggtagcata gaccagatca
     6541 acaatgtaat tttttgcctt gtcatcgaca taaatgcggt tcaccacttc ccgcgcctgc
     6601 agcagctgct ctttggtgac gacggtggaa acaccagccg gatgcagcaa atccacgcgg
     6661 tccataattt cccgctcttc ctgacggctg ggataggtta cccgaagctt cagcataaaa
     6721 cggtccacct gtgcttccgg gagcgggtag gtgccttcct gctctatggg attctgcgtt
     6781 gccagaacca taaagggctc atccagcgca taggtatttt cgcctatagt cacctgacgt
     6841 tcctgcatgg cttcaagaag ggcgctctgg acttttgccg gtgctctgtt gatttcatcg
     6901 gcaaggataa tattgccgaa aacaggccct ttgcgggtga caaattcacc ggtcttaggc
     6961 gtatatatca aagtaccgat gagatccgca ggcagaagat ccggcgtaaa ttggatccgg
     7021 cggaaactgc agtccatggc atgcgccaac gtggtgacgg cagttgtttt tgcaaggccg
     7081 ggcacacctt cgatcagcac atggccgttg gcaagcatcg ccgtcagcag acgatctatc
     7141 atatactgct gccccacaat aatgcggccg atctcttc
//
LOCUS       DAOPWB010000147         7174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10438_length_7174_cov_5.545301, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000147 DAOPWB010000000
VERSION     DAOPWB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_7174_cov_5.545301"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKN92_11265"
     CDS             <1..187
                     /locus_tag="PKN92_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49414.1"
                     /translation="AFVMTRLCANAEIGVPRGAAFSLWCGTDYYPLLWSIVKFPLAEH
                     IFLLITGDLYWFHTKML"
     gene            435..1514
                     /locus_tag="PKN92_11270"
     CDS             435..1514
                     /locus_tag="PKN92_11270"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNZ49415.1"
                     /translation="MDTPGSEKKWMIYGVTGYTGKHCAEEAVRRGLQPVVAGRNRKLV
                     EAAAAKLNLPCAVFSLDQPDKAAAALQDMDLVLNCAGPFSATIQPFLEACVRSRCHYL
                     DVTGEIPVFEYVHLNTERWKEAGIAVVPGVGFDVVPTDCIAALLKEALPDAVQLRMAF
                     KSKTGKVSPGTAKTILEGFSKGGMIRHEGRLVSIPSGSLVRKIPYADGEYLSVSIPWG
                     DVSTAYYSTGIPNIELYTAMPEDRLQLVRLMRYLAPLVRLRPVQRLIKNQIAQTVKGP
                     TEAERLADTSELYGVATNGAGERKEIWMSAPNGYTLTFDAAVSAAERVLEEEGFAGAY
                     TPSQAFGADFAGTLEGVSLRFSEES"
     gene            complement(1533..2621)
                     /locus_tag="PKN92_11275"
     CDS             complement(1533..2621)
                     /locus_tag="PKN92_11275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ49416.1"
                     /translation="MLTVAHLDEQDTLRGGEQQATWLVRGLLDQGVRCLVIGRPDRPF
                     ISMHPEEKELIRVPLPLRGELDLYSAWKLAGIVNRYQVDIVHAHASHAHGLAVLARCF
                     GGRHRVVVSRRVDFRPKGHWLNRWKYTRADRILCVSEKVAQTLNEYGLHAPQVKTVCS
                     AVDEARADMPPVSREALGIPDGIPFLFSAGSLVDHKDHANLLEAFALVSTEFPEARLC
                     IAGEGPLREALEQKRDSLGLQASVLFLGYRADAPGITRSADLYISSSWSEGLGTSILE
                     ALASGTSVVAAEAGGAREMVIPGKTGRLVPVRDARALADAMLLSLRNPQEAQAMAQRA
                     VSFVRDRFSVARMVAGSLEAYRELIQDA"
     gene            complement(2615..3775)
                     /locus_tag="PKN92_11280"
     CDS             complement(2615..3775)
                     /locus_tag="PKN92_11280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ49417.1"
                     /translation="MEKTPLHIMIADPHLQGGGQVRYVATLAQGLHEKGHRVTIACRP
                     GSVLADHARGCATALDRFQFRGGLRMRSWTNDHREALRFIRNEKPDILHVNGSQDHWT
                     FAFANGMLGKPVPLVRTRHNTYKVKTNLPNRILNRKHTDFQIVVCHSVRETLSRHPAF
                     CADRMLTIHNGVDAHRYKADTEARRRARAEFHYSDNDIVCGIVARLVPAKGHTFLFQA
                     AALLQEKHPELRIAVLGQGVLEEALRKEAETLGIADRVQFLGFRDDMNYCVQAFDIGA
                     QPSIDCDTSSFSMKEQMAAGIPLIASDYGGLTEIIDDGVEGLIVPAGTVEPLAAALER
                     LLSDASLRTAMAEKGKARVAAEFSSEVFVSRTLEVYQQLAAEKRRRKESASC"
     gene            complement(3833..4054)
                     /locus_tag="PKN92_11285"
     CDS             complement(3833..4054)
                     /locus_tag="PKN92_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49418.1"
                     /translation="MSKKDKIQITRTDDYAEMDGELTRAMEALDATNLRIEALLEDQL
                     RPVAQETGDTGETAEGATQTQQQEEQQEN"
     gene            complement(4109..4939)
                     /locus_tag="PKN92_11290"
     CDS             complement(4109..4939)
                     /locus_tag="PKN92_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNZ49419.1"
                     /translation="MIRRILAATDGSEASMAAVRYASLLAKKHGAAMTLLYVVDIKLL
                     EGPVVRDLSSSLGSVPFMNYQGIISRLLEDRGEEALKKTGALCSNEGISTEILLRTGL
                     VHRVIVEHAHLADLIVMGRSGEHTPFLEGLMGSTTEAVVRRAPIPVLIVSGKVSDSET
                     MVVAYDGSRQAKKALRFAAELAEHEKRPLQLLAVGDEAAEAWLNEARDYLHAHPNAES
                     YTQRSGEPETEILRFVEEQNAGLLVMGAYGRGKLYELVLGSTTSHVLNHANCPVLLVR
                     "
     gene            complement(4936..5544)
                     /gene="hisB"
                     /locus_tag="PKN92_11295"
     CDS             complement(4936..5544)
                     /gene="hisB"
                     /locus_tag="PKN92_11295"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010348670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNZ49420.1"
                     /translation="MTINTNGRYGRIQRKTRETAIEIEVNLDGTGQAEIETGVGFFDH
                     MLDHIARHGLLDLRVRAEGDLNVDPHHTVEDTGICLGQALLQALGTPKGITRYAHAVV
                     PMDETLAQATIDISGRPYLVFRGELPAGSPGGFDAELGEEFFRAVAVASRMTLHLELH
                     YGSNLHHCLEAMFKAFGRALREAVSLDPRITGVPSTKGMLEL"
     gene            complement(5558..6283)
                     /locus_tag="PKN92_11300"
     CDS             complement(5558..6283)
                     /locus_tag="PKN92_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNZ49421.1"
                     /translation="MLYAIMSDVHANIDALEKVLEKIDTLGVDHIICLGDVVGYNATP
                     NECVELLAARDIQTILGNHDAVACGLEEPWGFNPVALAAAIWTREQLSDESEEWLYSL
                     PDSLSYGHFTCVHGAPKNHNVYLFAWEDTLPHKYFLEEQNCNICFFCHTHMPVIFAEE
                     GPVPVTGNSPMKLEQGKPYFINPGSVGQPRDENAAASFALFNPDELIYELVRVPYDTE
                     RASKRVRDAELPPFLAERLVLGR"
BASE COUNT         1512 a         2000 c         2018 g         1644 t
ORIGIN      
        1 tgccttcgtc atgacccgct tgtgtgccaa tgcggagatt ggcgttccca ggggagccgc
       61 tttcagtctt tggtgtggaa cggactatta cccgcttttg tggtcaatag tgaaattccc
      121 tttagcggag catatttttc ttctaattac cggagactta tattggttcc acaccaaaat
      181 gctttgactt aaaacataaa atccgccagc ctgggaacgc caatctccgt attggcaaga
      241 ggcatgtgag gtttatggtt ccgggtgtac ttccgcggag ggcagtcagg gtatgacaaa
      301 acagagccgg ctgtggacaa gagagaagcg gcatccagaa ttttaaggcg aaaaaatctg
      361 ttgagttgag atagaatgga aaggcagcgt tacattctgt ttcggtctgg ccagcgacga
      421 aaggacggta cggcatggat actccgggca gtgaaaaaaa atggatgatt tacggcgtga
      481 ccggttatac ggggaagcac tgtgcagaag aggcggtgcg ccgtgggctc caacctgtgg
      541 tggcgggacg gaaccggaaa ctggtggaag cagcggcggc gaaactgaat ctgccctgtg
      601 ctgttttttc actggatcag ccggacaaag ccgccgcagc actgcaggac atggatctgg
      661 ttctgaactg cgcaggtcct ttttccgcca cgattcagcc ttttcttgaa gcctgtgtcc
      721 gcagccgctg tcattatctt gacgtcaccg gggagatccc tgtttttgaa tatgtgcacc
      781 tgaacacgga gcgatggaag gaagcaggca ttgccgtagt gcccggcgtc ggttttgatg
      841 tggtgccgac cgattgcatc gccgctcttc tgaaggaggc actgcccgac gcggtgcagc
      901 tgcgaatggc ttttaaatcg aaaacaggca aggtaagtcc cggcacggca aaaacaattc
      961 tcgaaggatt ttccaaaggc gggatgatcc gccatgaggg caggctggtt tccattccgt
     1021 cgggctctct tgtccggaaa atcccctatg ccgacgggga gtatctgtcg gtgtcgattc
     1081 cctggggtga tgtgtccacg gcgtattatt ccacggggat ccccaacatt gaactctata
     1141 cagcaatgcc ggaagaccgt cttcagctgg tcaggctgat gcgttatctt gcgcctctgg
     1201 tgcgcctgcg cccggtgcag cgcctgatca aaaatcagat cgctcagacg gtgaagggac
     1261 ccactgaagc agaacggctt gccgacactt ccgaacttta cggcgttgcc acgaacggag
     1321 caggtgaaag aaaagagata tggatgagcg cgcccaatgg ctatacactg acttttgatg
     1381 cggcagtgag cgcggcggaa cgggtactcg aagaagaggg gttcgccggt gcctatacac
     1441 ccagccaggc cttcggtgcg gactttgccg ggacgctgga aggggtcagt cttcgttttt
     1501 ccgaggaatc ctgatgcata cacacggatc cgttatgcgt cctggataag ttccctatag
     1561 gcttccaggc ttcctgcgac catgcgcgct acagaaaaac ggtcgcgaac aaaggaaaca
     1621 gcacgttgag ccatggcctg cgcttcttgg ggattgcgca gcgagagcag catggcatcg
     1681 gcaagggcgc gggcatcgcg aaccggtacc agtcgccctg tttttcccgg aatcaccatt
     1741 tcccgtgctc ctcccgcctc ggcggcaacc acagacgtac cggaggcgag ggcttcgaga
     1801 atggaagtgc cgagtccttc cgaccagctt gaagagatat acagatcagc ggagcgtgtg
     1861 attccgggtg catcggcgcg gtaccccaga aacagcaccg aagcctgcag tcccaggctg
     1921 tccctctttt gctccagcgc ttcccggagg ggaccttcgc ctgcaatgca gaggcgggct
     1981 tcggggaatt cggtgctgac cagtgcgaaa gcctcgagca ggttggcgtg gtctttatga
     2041 tccaccagcg atccggctga gaaaagaaaa ggaattccgt cgggaatacc cagagcctca
     2101 cgggaaacgg gaggcatatc cgcccgggct tcatccacgg cactgcagac tgttttcacc
     2161 tgcggcgcat gcagcccgta ctcgttcagc gtctgtgcca ctttttcgga tacgcacaga
     2221 atccggtctg ccctcgtata tttccagcga ttgagccagt gtcctttcgg ccggaagtcg
     2281 acccgccgcg atacgaccac ccggtgcctg ccgccgaagc accgtgccag tacggcaagt
     2341 ccgtgagcat ggctggcatg ggcatgcaca atgtccacct gataccggtt gacgatgccc
     2401 gccagcttcc aggcggaata aagatccagc tcaccgcgaa gcgggagcgg gacccggatc
     2461 agttctttct cttcggggtg catgctgatg aatggccgat ccggtcttcc gatgacaaga
     2521 cagcgcacgc cctgatccag cagcccccgc accagccagg ttgcctgctg ttctccgcca
     2581 cgcagcgtat cctgttcgtc cagatgagca acggtcagca tgatgctgat tcctttctcc
     2641 gccttttttc agcggcaagc tgctgataca cttcgagggt gcgcgataca aaaacttcac
     2701 tgctgaattc ggcggcaact cgggctttcc ctttttccgc catggcggtg cggagcgatg
     2761 catcggaaag aaggcgctcc agcgctgccg caagcggctc cactgtaccg gcaggcacga
     2821 taagcccttc cacgccgtca tctataattt ccgtgagacc gccgtagtcc gaagcaatca
     2881 gcgggatgcc tgccgccatc tgttctttca tactgaaaga agaggtgtcg cagtcgatgg
     2941 aaggctgggc tccgatgtca aaggcctgca cacaatagtt catgtcatct ctgaatccga
     3001 gaaactgcac ccggtcagct atgccgagtg tctccgcttc tttgcgcagt gcctcttcca
     3061 gcacgccctg tcccagcacg gcaatacgca gctccggatg cttttcctgc aggagagcgg
     3121 ccgcctgaaa aagaaaggta tgcccttttg ccggtaccag tcgtgccaca atgccacaga
     3181 cgatatcgtt gtcgctgtaa tgaaattccg cccgggcacg gcgccgcgcc tccgtatcag
     3241 ctttgtaccg atgtgcatcc acgccgttat gaatggtgag catgcggtcg gcacagaaag
     3301 cgggatggcg tgagagagtt tcccgcacgg aatggcagac aacaatctga aaatcggtat
     3361 gttttctgtt cagaatgcga ttcggcagat tggttttgac tttgtaggta ttgtgccgcg
     3421 tgcgtaccag cggcaccggt ttcccgagca tgccgttggc gaaggcaaaa gtccagtgat
     3481 cctgcgagcc gttcacgtgg aggatgtcgg gcttttcatt tcggataaaa cggagggctt
     3541 cccggtgatc attcgtccaa ctgcgcatac gcagaccgcc ccggaactgg aaacgatcca
     3601 gcgcagtcgc acagcctctg gcgtgatctg cgagtacgct gcccggtcgg caggcgatgg
     3661 ttacccggtg tcctttttca tggagcccct gcgccagcgt cgccacatag cgtacctgtc
     3721 cccctccctg caggtgaggg tcggcaatca tgatatgcag aggcgttttt tccatagaca
     3781 tcctgttcag cgggctgcct gcggggcgcc gaagcgattg atgccgccgt gttcagtttt
     3841 cctgttgctc ttcctgttgc tgagtctgtg tcgccccttc agcggtttcg cctgtatcac
     3901 cggtttcttg tgcgacaggg cgcagctgat cttccagcag cgcttcaatg cgcaggttgg
     3961 tggcgtccag tgcttccatg gcacgggtca gctcgccgtc catttccgca taatcgtcgg
     4021 ttctggtgat ttgaatttta tctttttttg acataatgct tttataccgg ctggtttccg
     4081 tttcaatgcc ttccggtcga atcgatgctc agcgtaccag aaggacagga cagtttgcgt
     4141 gattcagcac gtgcgaggtg gtgcttccca ataccagttc gtacagtttc cctctgccgt
     4201 aagcgcccat aaccagcagc ccggcgttct gttcttcgac aaaacgaagg atttctgttt
     4261 caggctcacc gcttctttgt gtataacttt ccgcattggg atgggcatgc aggtaatccc
     4321 gtgcttcgtt cagccaggcc tccgcagctt catctcctac agccagcagc tgcaggggac
     4381 gtttttcatg ttctgccagt tcggcggcaa atcgaagcgc ttttttcgcc tgccgggatc
     4441 cgtcataagc gacgaccatg gtttcggagt ccgagacttt cccgctgaca atcaggacgg
     4501 gtatgggcgc gcggcgtaca acagcctcgg tcgtggaacc catgagccct tccagaaaag
     4561 gggtgtgctc tccgctgcgt cccataacga tcagatccgc cagatgggca tgttccacaa
     4621 tcacgcgatg gacaagcccc gtccgcagga gaatctcagt ggaaatacct tcattgctgc
     4681 agagagcccc tgtctttttg agcgcttctt caccgcggtc ttccagcagg cggctgataa
     4741 tgccctgata gttcatgaag gggacggagc ccagcgaaga ggagagatcg cggacaacgg
     4801 gcccttccag cagtttgatg tccaccacat aaagaagagt catggctgca ccgtgctttt
     4861 tcgccagcag cgatgcatag cgcactgccg ccatagatgc ttcacttccg tctgttgcag
     4921 ccagaatacg tctgatcata gttcgagcat gcctttagtg gagggtacgc ctgtgattcg
     4981 gggatctaag gagactgctt cgcgcaatgc cctgccgaag gctttgaaca tggcttcgag
     5041 gcaatggtgc aggttggagc cgtagtgcag ttccagatgg agggtcatcc ggcttgcgac
     5101 ggcgacagca cggaagaatt cttcaccgag ctcggcatcg aagccgcccg gactgccggc
     5161 aggcagctca ccgcgaaata caagataggg ccgccccgag atgtcaatgg ttgcctgtgc
     5221 cagcgtttca tccatgggta cgacggcatg ggcgtaacgt gtgatgcctt tgggcgtgcc
     5281 cagcgcctgc agcagcgcct gtcccagaca aatcccggtg tcttccacgg tatggtgcgg
     5341 atccacattc agatcgccct ctgcccggac tcgcagatcc agcagaccgt gccgggcgat
     5401 atgatccagc atatgatcga aaaaaccgac tcctgtctca atttcagcct gaccggttcc
     5461 gtccagattg acctcaatct caatggcggt ttcacgggtc ttacgctgga ttctgccata
     5521 gcgcccattg gtgttgattg tcatggagcg gctcctttta tcggccgaga accagccgtt
     5581 ctgcgaggaa gggcggtaat tccgcatcac gtacccgttt gctggcacgc tctgtatcat
     5641 agggcacacg caccagttca tagatcagct catccggatt gaacagagcg aaagatgcgg
     5701 ctgcattttc atcgcggggc tggcctacag aaccggggtt gataaaatag ggtttacctt
     5761 gttccagttt catcggcgaa ttgcctgtta ccggcacggg cccttcttct gcaaaaatca
     5821 caggcatgtg cgtatggcaa aaaaaacaga tgttgcagtt ctgttcttcc agaaaatatt
     5881 tgtggggcag cgtgtcttcc cacgcaaaga gatacacgtt atggtttttc ggcgcaccgt
     5941 gcacgcaggt gaaatgaccg tagctcaatg agtccggaag gctgtaaagc cattcctcgg
     6001 attcatcact cagctgttct cttgtccaga tggcggcggc aagggcaaca gggttgaaac
     6061 cccagggctc ttcaagcccg caggcaacag cgtcatgatt gccgagaatg gtctggatat
     6121 cccgggctgc aagcagctca acgcattcat tgggggttgc gttgtagccg acaacatcac
     6181 caaggcagat gatatgatcc acgccaagcg tgtctatttt ttccagcact ttttccagtg
     6241 cgtcaatatt tgcatgcaca tccgacatga ttgcatataa cacagcgatg aactcccgtc
     6301 ccgcgctcgc agacggcacg cgaaagcaat gccgcccgaa actgttttca ggtaaagttt
     6361 gaattaaagg tatattctgc acactctgaa ggggagtaat caaattatgg ggagtgcgtg
     6421 atttcactgc agtgcctttg caggcctgag ggaatgcgtt ggttattatc cgcctgtgat
     6481 cggatgggtg ggtatctttc tttatgttat tacctgtgac ttatcttgcc ccgatgccaa
     6541 agccctctga cgcaaaacat aaaatccgcc cggccgggga gaggcaacga cagcttgata
     6601 cggctgagag caccgtccac tgtgtccacc ctgtccactg attttggtgt ggaatgcaat
     6661 cctgtgaagc aaaggcgtcc atgcccgaat caagactcct gcgcacaccg ccgttacgtc
     6721 caccctccgg cgtcattcct gcgcacgtag ccgttacgtc caccctccgg cgtctctgcg
     6781 cacgtcgccg ttacgtccac cctccggcgt cattcctgcg catgtcgccg ttacgtccac
     6841 cctccggcgt catccctgta cacgccgccg tcacgtccac cctccggcgt cattcctgcg
     6901 caggcaggaa tcttaacgcg agttgttctt tatgagggac ttaccgtggt cgacaactac
     6961 gcggaaatac cgtcaagaca tatctcatac gggaagtgtg gcgagttgtg tgtccttcag
     7021 tctttggtgt ggatcagggc gatatataat tcatggcgtc catgccctgg ttaagattcc
     7081 tgccttcgca ggaatgacgg gggaaaggca ggaatgacag gggaaaggca gttcttcccc
     7141 tgggaacgcc aatctccgca ttggcacaca agcg
//
LOCUS       DAOPWB010000148         7173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10442_length_7173_cov_5.978786, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000148 DAOPWB010000000
VERSION     DAOPWB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10442_length_7173_cov_5.978786"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..1073
                     /locus_tag="PKN92_11305"
     CDS             312..1073
                     /locus_tag="PKN92_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNZ49422.1"
                     /translation="MMSYVILTPEGKLIVLDGGMKCDAPYLREFIAARASEVELWIIT
                     HPHLDHMDALGVLFDTGTAPKIKKLFASFPELSWVKDHCSDGEYASFLAFSESLSQSG
                     VLCEDAQPDQKFKIDRLEFHVLGVRNPELTRNPLNNSSLVIRVSDGVKSVLFLGDLGL
                     EGGEKLLKSPLAESLPSEYVQMAHHGQAGVGESFYQKVNPSYCLWTTPKWLWDNDRGE
                     GPGTGPYKTLETRAWMDKLPIKKHYIMYEGLHEIN"
     gene            complement(1070..1510)
                     /locus_tag="PKN92_11310"
     CDS             complement(1070..1510)
                     /locus_tag="PKN92_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNZ49423.1"
                     /translation="MTASRNLYEQVILDHNRTPRNYGELVNPDATVEGVNPLCGDSFV
                     LYACFDGDRISDIRFSGHGCAISKASASLMTTVVKGESRQKAQQLFEHFHAMLQSDAL
                     VAPDEETLGKLIVFAGVREYPMRIKCAMLAWHALHQLLKDNDPS"
     gene            complement(1507..2790)
                     /locus_tag="PKN92_11315"
     CDS             complement(1507..2790)
                     /locus_tag="PKN92_11315"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNZ49424.1"
                     /translation="MTTIIENSKPSLYERQPEISTRFPVHQVREDFPILSSEVRGRKL
                     VYLDNAATTQKPQSVIQAVTRYYKGENANIHRGVHYLSEQATQKYENARRKVGAFIGA
                     TVNCEVIFTRGTTESINLVAQSYGRAFVREGDEIIISALEHHSNIVPWQMLCQEKGAI
                     LRIIPLLENGELDLGAFDKLLSSKTKVVAVTQLSNVLGTLVPVRQICKKARDAGAVSL
                     VDGAQSTPHLSVDMQQIGADFFAFSGHKLYGPTGIGVLYGRAELLERMPPYQGGGDMI
                     RSVSFEHTEYADLPGKFEAGTPNIAGAIGLGAAIDYLQSLGMERIAAYEAELDGYTRS
                     RLQEVEGLQIQGNPEEHRGAVSFTLDGVHPHDIGQLLDQEGIAIRAGHHCAQPLMKCL
                     GVPATARLSAGLYNTREEIDMLASSLQGLRTLFHP"
     gene            complement(2787..4103)
                     /locus_tag="PKN92_11320"
     CDS             complement(2787..4103)
                     /locus_tag="PKN92_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNZ49425.1"
                     /translation="MTTPLKEHYLAEMTRFQNGAAAEAVTHIRSRGKSAFQQMDFPGT
                     KDEAWRQTNIKPILAHPFFLTGETQTDDAEIKKQLALLPIGPDDYFLVTVNGRFSLRH
                     SRLPSVSHGYLGGLKEAVAEDMGATFTDLLQRSAYPAHAFLALNDAFLSDGTAILLEE
                     NVKLDHTLHIVCLTGAAEDASAIHERCLIRLKQGARACIAVHHAAIGHAPTVLHTFVE
                     EYVLDRDSALIRLESLQDRADDYRISRLTVKQKENSRLKSFSFALDPGKITRNEIQVE
                     LQEAGCTSKLYGMALNTEKKLLDNDLLIHHRAPSCTSRMRYKQMLNGSSRGVFTGKIH
                     VDKDAQHTNSEQLSANILLSGQARMDAKPQLEIYVDDVRCTHGVTVGRPDPLALFYLR
                     SRGIDSPLGTKMLSFGFAEDVISRVPDESLKNWFSAEFWKRIQRET"
     gene            complement(4114..4881)
                     /gene="sufC"
                     /locus_tag="PKN92_11325"
     CDS             complement(4114..4881)
                     /gene="sufC"
                     /locus_tag="PKN92_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNZ49426.1"
                     /translation="MLSIVDLHASVNDQLILKGLNLQINPGEVHAIMGPNGSGKSTLA
                     QVLAGHENYTVDETRGSVVFQGEDLLALSPEERARQGLFLAFQYPVEIQGITNSSFLQ
                     TALNEIRKSRGEEEMDALDFAQFLKKKMELVEMQEDFAERFVNFGFSGGEKKRNEILQ
                     LAVLEPALAILDETDSGLDIDALRTVASGVNRLRSPQRSFLLITHYQRLLDYIQPDFV
                     HVLYDGKLIQSGGKELALKLEEQGYNWIREAEETLPA"
     gene            complement(4881..6296)
                     /gene="sufB"
                     /locus_tag="PKN92_11330"
     CDS             complement(4881..6296)
                     /gene="sufB"
                     /locus_tag="PKN92_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005772922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNZ49427.1"
                     /translation="MVRKRYEHGWTTAIESDSFDKGLNEDVIVALSRIKNEPDWMTEW
                     RLNAFRLWQQMEIPRWAKLVYPDPDFQDISYYSAPKSAVSPRSLDEVDPKLVETFTKL
                     GIPLEEQKRLSGVAVDAVFDSVSVATTHQQILKRQGIIFCPISEAIQEHPDLVQKYLG
                     SVVPQGDNFYASLNSAVFSGGSFVYIPAGVTCPMDLSTYFRINAKHTGQFERTLIIAE
                     EGSKVSYLEGCTAPIRDENQLHAAVVELVAHKDASIRYATVQNWYAGDDDGKGGIYNF
                     VTKRGACLGDRSHISWTQIETGSAITWKYPSCLLKGKGSVGEFYSVALTKGKQQADTG
                     TKMIHTGADTRSTIISKTISAGKSDSSYRGLVRVMPGAENARNYTQCDSLLMGNDCGA
                     HTWPYIDVRNDSASVEHEASTSKINEDQLFYCQSRGIDREDAISLVVNGFCKEVLDHL
                     PMEFAVEASRLLSVTLEGSVG"
     gene            6681..>7173
                     /locus_tag="PKN92_11335"
     CDS             6681..>7173
                     /locus_tag="PKN92_11335"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016193095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNZ49428.1"
                     /translation="MQRAVENVNVLSLTPLPPPDELKAQFPLTENTADTVANCREQLA
                     AILDGKDRRFLVITGPCSIHDTEAGLDYARRLHALSRELSDRLFILMRVYYEKPRTTV
                     GWKGLINDPDMNNSYDIQKGLRKARRFLLDVAEMGLGAASEVLDPIIPQYLADLMSWV
                     SIGA"
BASE COUNT         1741 a         1748 c         1784 g         1900 t
ORIGIN      
        1 aaaccataaa attcgccagc ctgcgaacgc caacgaccta ttggcagagg cttttaaagt
       61 caggcaaaat taacgggata gacgatgaaa tgattttttt tcgtggtctg tccgtgcatt
      121 tatctgttac catactgaga tactatagaa gaccaaaaac gaaaggagta tctatcatga
      181 ggagttatcg ttactgtttg atactgccag tctgtacggc gttatttttt tctgcagttt
      241 ttgcttctca aaattgtgtc ggcgatgaga gctttacttt atggcagctg ccgagccagg
      301 gacccacaca aatgatgtcc tatgtcattt taacgcctga aggtaaattg atcgtgctgg
      361 acggcgggat gaaatgtgac gctccctatc ttcgggaatt tattgcggcg cgggcatcgg
      421 aagtggaact atggatcatt acccatccac atttagacca tatggacgcg ctgggcgtgc
      481 tgtttgatac tggaactgct ccgaagatca agaaactgtt tgcgtctttt cctgaattgt
      541 catgggtaaa agatcattgc agcgacggag agtatgcgtc ttttcttgct ttcagtgaga
      601 gtctttctca atccggggtt ctctgcgagg atgcgcagcc tgatcagaag tttaaaatag
      661 accggttgga atttcacgtt ttgggtgttc gcaaccctga gctcacccgc aatcccttaa
      721 acaactcatc tctggtaatc cgggtcagcg acggcgtcaa gtctgttctg tttctagggg
      781 atctgggact ggaaggcggt gaaaaactgc tgaaaagccc gcttgccgag tcgttgcctt
      841 cagaatatgt gcagatggca catcacggtc aggcaggcgt gggagaatcg ttttaccaga
      901 aagtcaaccc gtcctattgc ctgtggacga cgcccaaatg gctgtgggat aacgaccggg
      961 gagaaggccc gggaacggga ccttacaaaa cgctggaaac acgggcgtgg atggataagc
     1021 tgccgattaa aaaacattac atcatgtatg aaggactgca cgagatcaat taagaaggat
     1081 cattgtcttt cagcagctga tgcagcgcat gccacgccag catggcgcat tttattcgca
     1141 tgggatattc ccgaacgcct gcgaaaacaa tgagtttgcc cagcgtttct tcatcaggag
     1201 caacaagggc atcactttgc agcattgcat gaaaatgctc aaaaagctgt tgcgcttttt
     1261 gtctgctttc ccctttgacc acagtggtca tgagagacgc actggctttg gaaatggcgc
     1321 agccgtgacc tgaaaaacgg atatctgata tgcggtctcc gtcaaaacag gcatagagca
     1381 caaaggagtc accgcaaagc gggttaacgc cttccacggt tgcgtccgga tttaccagct
     1441 ctccgtaatt gcggggggtc ctgttgtgat ccagaattac ctgctcatat aagtttcttg
     1501 aagcggtcat ggatggaaaa gtgtccttaa gccttgaaga cttgaagcca gcatatcaat
     1561 ttcttcacgg gtattataca accctgcact cagccgggcg gtagcgggaa cgcccagaca
     1621 tttcatgagc ggctgcgcac aatggtgccc agcccgtatg gctatgcctt cctgatccag
     1681 cagctggccg atatcatggg gatggacacc atccagcgtg aaggaaacgg cgccccggtg
     1741 ttcttccggg ttgccctgga tctgcagccc ttccacctcc tggaggcggc tgcgggtgta
     1801 accgtcaagt tctgcctcat aagcggcgat gcgttccatt ccaaggctct gcaggtaatc
     1861 aatggcggcg cccagtccga ttgcgcctgc aatattcggc gtccccgctt caaatttgcc
     1921 ggggagatct gcgtactccg tgtgctcaaa actgacggag cggatcatgt cgcctccgcc
     1981 ctgataaggc ggcatccgtt ccagcagttc tgctctcccg taaaggactc ctattcctgt
     2041 aggcccgtag agtttgtgtc cggaaaaggc aaaaaaatcg gctccaatct gctgcatgtc
     2101 aacagaaaga tgcggggtgc tctgtgctcc atcgacaaga gaaacagcgc ctgcatcgcg
     2161 cgctttcttg caaatttgcc tgacgggaac aagcgttccc agaacattgg acagctgggt
     2221 tactgcaaca acctttgtct ttgatgacag taatttatcg aatgcaccca gatccagttc
     2281 accattttcc agcagaggga taatgcggag gatagcccct ttttcctgac agagcatctg
     2341 ccacggcaca atgttggagt gatgctccaa cgccgagata ataatttcgt ccccttctct
     2401 gacaaaagcc ctgccatagc tttgcgctac aagattgatg gactctgtgg ttccgcgtgt
     2461 aaaaataact tcgcaattaa cggtggcacc tataaaagcg ccaactttgc gtcgcgcatt
     2521 ttcatatttt tgggtagcct gctcgctgag atagtgaacg ccccgatgga tgttggcgtt
     2581 ttcacctttg taatatcggg taacagcctg aatcacactt tgtggctttt gtgtggtggc
     2641 agcattatcc agataaacca gttttctgcc tcgcacctct gaagagagaa ttgggaaatc
     2701 ctctcgcacc tgatgtacag gaaagcgtgt ggagatctcc ggctgccgct cgtataaaga
     2761 gggtttggaa ttttcaataa tagtggtcat gtttcacgct ggattcgttt ccagaattct
     2821 gctgaaaacc aatttttcaa cgattcatca ggaacacggc tgataacatc ctcagcaaaa
     2881 ccgaagctga gcattttcgt tcccagaggg gaatcgattc ctctggaccg caggtaaaaa
     2941 agcgccaagg gatcagggcg tcccacggta accccgtggg tgcaccgtac atcgtcaaca
     3001 taaatttcaa gttgaggttt tgcgtccatg cgcgcctgtc cggacagaag gatattggcg
     3061 ctcagctgct ccgagttggt gtgctgtgca tctttatcca catgaatttt tccggtaaac
     3121 acaccgcggc tgctgccgtt cagcatctgt ttgtaccgca tgcggcttgt acaggacggt
     3181 gcgcgatggt ggatgaggag atcattatcc agaagttttt tctccgtatt cagcgccatg
     3241 ccgtacagct tggaagtgca tcccgcttcc tgtagttcga cctgaatttc atttcttgta
     3301 attttaccgg ggtcgagggc aaaagaaaaa gatttcagac ggctattttc tttttgtttg
     3361 acggttagcc tgctgatacg gtagtcatca gcccgatcct gcagcgactc caggcgaatc
     3421 aatgcggaat ctctgtccag aacatattct tcgacaaagg tatgcaaaac cgtcggggca
     3481 tgtccaatag cggcatgatg aacagcaata catgcacggg caccctgttt caatcgaatg
     3541 aggcaccgtt cgtgaattgc cgaagcatct tctgcagcac cggtgagaca gacaatatgc
     3601 aaggtgtgat ccagttttac attttcttct aaaaggatgg cagtcccatc gctaagaaaa
     3661 gcgtcattta aggcgagaaa agcgtgagcc ggataagcac ttctctggag aagatcggtg
     3721 aatgtggctc ccatgtcctc tgcaacggct tccttcaatc cgcccagata gccatggcta
     3781 actgatggca gccgggaatg gcgaagagag aaccgcccgt ttaccgtaac cagaaaataa
     3841 tcatcggggc ctatagggag caaggcgagc tgctttttaa tttcagcgtc gtccgtttga
     3901 gtttcgccgg taagaaaaaa aggatgcgcc agaatcggtt ttatatttgt ctggcgccac
     3961 gcctcgtctt ttgtgcccgg aaaatccatc tgctgaaagg cagattttcc tctgctgcga
     4021 atgtgggtaa cagcctccgc tgcggccccg ttttggaaac gagtcatctc tgccagatag
     4081 tgctctttta gaggagtagt cataaagtac gccttatgcc gggagcgttt cttctgcttc
     4141 acggatccag ttatacccct gctcttccag tttcaaagca agctcttttc cgcccgattg
     4201 tatgagtttt ccgtcataga gcacatgaac aaagtccggc tgaatataat ccagcaggcg
     4261 ttgatagtgt gtgatgagca ggaatgacct ttgtggggaa cggagcctgt ttacaccgga
     4321 agccacggtg cgtagtgcat caatatcgag gccggaatcg gtctcgtcca ggatggcaag
     4381 agcaggttcc agcacggcga gctgcaggat ttcattccgt tttttctcgc cgcccgaaaa
     4441 gccgaaattt acgaagcgtt cggcaaaatc ttcctgcatt tccaccaatt ccattttctt
     4501 tttcaaaaat tgtgcaaaat ccagcgcatc catttcctcc tctccccggc ttttccgaat
     4561 ctcattcagt gcggtttgaa gaaagctgct gttggtaatt ccctgaatct ccacgggata
     4621 ctgaaatgca aggaaaagcc cctggcgtgc ccgttcttca ggggataacg ctaaaagatc
     4681 ttccccctgg aatacaacac ttccccgtgt ttcatctacc gtgtagtttt catgcccggc
     4741 gagcacttgt gccaaggtgc tttttccgga gccgttcggt cccataatgg catgcacttc
     4801 tccgggattg atttgaagat tcaatccttt caggatcaac tgatcattta ctgaggcatg
     4861 cagatcaaca atactgagca ttatccgaca ctcccttcca gtgttacact taataaacgt
     4921 gatgcttcga cggcaaattc catggggaga tgatcgagca cttctttgca gaatccgttg
     4981 acgaccagcg agatggcgtc ttcccggtca ataccgcgtg actgacagta aaataactga
     5041 tcctcattaa tctttgaggt ggatgcttca tgttccaccg aagcgctgtc attgcggaca
     5101 tcaatataag gccaggtatg agcaccgcaa tcatttccca tgagcagact gtcacattgg
     5161 gtgtagttgc gcgcattttc tgctcccggc atgacacgta ccagaccccg gtaactgctg
     5221 tcgctttttc ctgctgagat agtcttggaa ataatggtac tgcgcgtatc tgcacccgta
     5281 tgaatcattt tggttcctgt gtctgcctgc tgtttcccct tggtcagggc aacagagtag
     5341 aactctccta ctgagccttt cccctttaaa aggcagctgg ggtatttcca ggtgatggca
     5401 ctgcctgttt ctatctgggt ccaggatata tgggaacgat cgcccaaaca cgcgccccgc
     5461 tttgttacaa aattatagat accgccttta ccgtcatcgt ctccggcata ccagttctgc
     5521 acagtggcat agcggataga agcatctttg tgagcaacca attccaccac ggcggcgtgc
     5581 agctggtttt catcgcggat aggcgccgtg catccttcaa gataacttac tttagagcct
     5641 tcttcagcga taatcagtgt ccgttcaaac tgtcccgtat gttttgcgtt aatccgaaaa
     5701 taggttgaca gatccatcgg acaggtaaca cctgcaggaa tatagacaaa ggaaccgccg
     5761 ctgaagaccg ccgagtttaa agaggcataa aagttatcgc cctgaggaac cacagagccc
     5821 aaatatttct gtacaagatc cgggtgttcc tggatggctt cggagatagg acagaagata
     5881 atgccctgcc gtttcagtat ctgctgatga gttgttgcga cggagacact gtcaaaaaca
     5941 gcatcaacag cgacaccgct taaccgtttc tgttcttcca gcgggattcc gagtttagtg
     6001 aaagtttcca ccagtttggg gtccacttca tccagactgc gcggcgaaac ggcggattta
     6061 ggagctgagt agtaggagat gtcctgaaag tccggatccg gatacacaag tttcgcccat
     6121 cggggtattt ccatttgctg ccacagacga aacgcgttca gccgccactc tgtcatccag
     6181 tccggctcat ttttgatgcg ggaaagcgct acgatgacat cttcattgag acctttatcg
     6241 aaactgtccg attcaatggc agtggtccat ccatgctcat agcgtttccg gaccatttcc
     6301 tgtacttcta tgttcttctg gttcactttg cgttagatcc tgaatggtaa gtatggcggc
     6361 aagctcgttt acggtctact ctttgcattt gcaaatcaaa caccagatct gattaaactc
     6421 ttgaaagtat agcggtatac taatgaataa acatgtattt cttttttctt tattccaaag
     6481 gctttgattg aactgtattt caatgagaaa aatacgggat gcgagataaa gtgcgggaat
     6541 tatttggtta agctgcaagt tttaagttac gatggaggtg acgattttca acgaaacatt
     6601 gtatttactg ttttctgata tcgttgcaag gcatccggtt tttgtttccc tgctgaatta
     6661 ctggaaaaga gatttttatt atgcagcgcg ctgtagaaaa tgtaaatgtt ttatctttga
     6721 ctcccctgcc gccgccggat gagttaaaag cgcaatttcc gcttacggaa aatacggcgg
     6781 atacggttgc caactgcaga gagcagcttg ctgccatcct ggacgggaaa gacaggcgtt
     6841 ttctggtgat tacgggtccc tgctcgattc acgacactga ggccggtctg gattatgcaa
     6901 ggcgcctgca tgcactgagc cgggagttgt ccgaccgtct ctttatcctg atgcgggttt
     6961 attatgaaaa accgcgcaca actgtcggat ggaagggtct tattaacgat ccggacatga
     7021 ataacagtta tgacatccag aaagggcttc gcaaagcacg tcgttttctg ctggatgttg
     7081 ctgaaatggg cttgggagcg gcaagtgaag tgctggatcc cattattccc cagtatctcg
     7141 ccgatctgat gtcctgggtt tctatcggag cac
//
LOCUS       DAOPWB010000149         7112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10555_length_7112_cov_4.778943, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000149 DAOPWB010000000
VERSION     DAOPWB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10555_length_7112_cov_4.778943"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>699)
                     /gene="cas1"
                     /locus_tag="PKN92_11340"
     CDS             complement(<1..>699)
                     /gene="cas1"
                     /locus_tag="PKN92_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNZ49429.1"
                     /translation="KVSSNLVLKWLFARKQETTVLHVTTPGARVGKAGDQISVTTPDG
                     EIQKFPLAQIESMVIHGYGQVTTQALQLCVLNRIPVHWMSASGFYITGAVPDSGQVQR
                     RIRQYEALRDESFKMSLAQKLVHAKVEGQLRYLLRATRSAPRSEAITKSISDLRKSLA
                     DIVKAESPDVLRGLEGMAGRAWFAVYPNTLLAAGAGEEFQIKGRSRRPPQDRYNALLS
                     FGYALLHRSVMQAL"
     assembly_gap    700..709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<710..1329)
                     /gene="cas4"
                     /locus_tag="PKN92_11345"
     CDS             complement(<710..1329)
                     /gene="cas4"
                     /locus_tag="PKN92_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNZ49430.1"
                     /translation="MTEILELPIRVMALHALEYCERLFYLEEVEEIRVADERVFAGRE
                     LHETLELQEGEKRQEFELSSDTLGIIGKLDAVRHREGGWVVYEHKRGRPRSPLRKGEP
                     YEAWDSDALQLYAYCMLLEEHLGKPITEGRIRYHAENITVRLPLDDAARCKVNQAVAR
                     ARELRSSLKRPAVTANERLCIRCSLAPVCLPEEERLACDPDWEPLRL"
     gene            complement(1326..2009)
                     /gene="cas5"
                     /locus_tag="PKN92_11350"
     CDS             complement(1326..2009)
                     /gene="cas5"
                     /locus_tag="PKN92_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HNZ49431.1"
                     /translation="MISLYVEAPFAVFRIFTAGYFRPTARFMPPSSAYGLLLNIAGIE
                     MRWDDGKSEMTLIRSSGLPGCRLALGTPAAPEVRVLYQQLHNYPVGAHRKENADRCKG
                     SKYNIKPVKRELLCDLKAYILVDGEESFEAAIRDGLSGKVKKRYGLPFLGDNNFLPNR
                     LDLTSPPEEIRWWERLDEDAGTEDGTVTTRMTQCIHRSDMTQTRSALYILGKPRADVP
                     ERAWTEMYL"
     gene            complement(2006..2947)
                     /locus_tag="PKN92_11355"
     CDS             complement(2006..2947)
                     /locus_tag="PKN92_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HNZ49432.1"
                     /translation="MNLFATVLTHAAPSANYRGESAENRAVIQKITKDRFEYAIISPE
                     AIRNALREGLRALGLPCNRERLEDEEQLAVQFTTFPDAEKWADDFFMGWLVAAGSKDR
                     TKYLKEIKEKEGRDPAAFTFKRDSILRMNMAVALEPYRHNTVFTQSPQQLNSPWQNAQ
                     NSQLLHRETVFTAYQYPFALNLEDCRKKPEWTRALLRAISQLNNVAGNHARSYFEMAP
                     ASVVVRLTPCLAAGYNTYGFEIADRTQTHLFPEVVEGILAGDFPGNEFYLGGKLVRDM
                     DEKTSSTLSQKGTVLDRNAARLLDLVGDQAFAAGEQS"
     gene            complement(2968..4689)
                     /gene="cmx8"
                     /locus_tag="PKN92_11360"
     CDS             complement(2968..4689)
                     /gene="cmx8"
                     /locus_tag="PKN92_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein Cmx8"
                     /protein_id="HNZ49433.1"
                     /translation="MTTSMIPEKAPPGATDQDSKHSEGLVLRYTLHDLPTAQHKAGLA
                     GLLLMLESMRRRKIAPIPEVVEVSAHGASIKVTAESLQSVFDDYYDGANIEIALKNKR
                     AGEEPLREDESETIDEDGKSKIEKRFIYEIIQPKAAFLSALNGDQVAWTKLYREVMWN
                     ILRGRPATQIPYKTRLEGVQQNESEKLWQALEKSQAKKKGPSLMPIASSIFIGAQAES
                     AEKIGFQGIPEENLLLHFWPLASRIFCPATVDLDGKTNDVGFVLVIPEPLDLQYFCEN
                     CLEMLASLGPELSGYRPSAARIDVPAEGGLEYLGALARHRADGSFRFSVSAVESYHLE
                     KVGNNVQMHGMQRIEASRQILDRYERFKQNFRNGLFKVQHLANLLADAPWYRGFISVM
                     AFMPAKFFIQKTGETPGKLSFFGRDAWYAFSLLQPNNPTKGGQPMSEEMKDELLAQRV
                     YEVVRTYVMSRTEDKSGITYDSFKENRDENGYVKTPESYRDAKEKVCMDAFLAIRGRT
                     EQDFVEYFAGTLCSVPQNLKKDRYVEISNALFTEWEKVKTLSLLALSAVSWSFKPITS
                     TSTFKED"
     gene            complement(4686..7025)
                     /gene="cas3"
                     /locus_tag="PKN92_11365"
     CDS             complement(4686..7025)
                     /gene="cas3"
                     /locus_tag="PKN92_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HNZ49434.1"
                     /translation="MTAATSIILAKSTRCPTAPLPHETLMGHSSAVADSFQALFLPDG
                     KTGWLTEQWFTFFQLSQQDHDIFLQHGLAAAWLHDLGKANNTFQAMLRHKSPGDGLNQ
                     ALYHEFLSGMILSMPEAGIFFPQSLNLELLITVVMSHHLRFDRSRFAKLGVHNTFQIN
                     VPVIESLLNQAFPPPPGNPSLSLSLSKHWSFYEQPDTCQVYKIADALRYRLEKYHRRL
                     RSDKKEVRLLRALRAALIISDSAGSGITRENKEVRDWLPPLFSKNQSLSEKEILDSVI
                     TPRISSITQKRSSSFQWSGFQEKAAELPDRTLLITPCGSGKTLAAWRWIAARLSARPA
                     SRVIFLYPTRATAAEGFRDYVSWAPESDAALISGSATFDLQGMFDNASDDRRGRSYLT
                     EERLFALGYWSRRIFSATVDQFLGFIQNVYASICLLPLLADSVLVLDEIHSYDKGLFD
                     ALKAFLKEFNLPVLCMTASLPGDRICQLTEELGLRLFPDPVHHGDEHFPDLQERSDKE
                     RYKVTLLAGEDEALKNARQALDQGLKVLWVVNTTERCRRLAEEMKALCYHSRFRLCDR
                     KKQHDQVIRAFQGEKQAVCAVTTQVCEMSLDLDADVLISEYAPITALIQRMGRCNRRD
                     LPQSNRIGEVFLYKPENDLPYDKKSLSGVTSFVSALTERLVTQKDLAELLGQLGPDQY
                     EAERWLSFINGGVNALSREESLRDGIDFMVQAVLDSDVPAYLELRKQRSPKSAGLLLP
                     VPRQEGKRDARLPDWLHIASSENYDPNLGYGPQARKEIL"
BASE COUNT         1705 a         1817 c         1811 g         1769 t
ORIGIN      
        1 gcagtgcctg catgacactc ctgtgcagca gagcatagcc aaagctgagc aaagcattat
       61 accggtcttg aggcggtctc ctgcttcgtc ctttaatctg aaattcttct ccagcacctg
      121 cagccagcag tgtgttcggg taaacagcaa accacgcccg acccgccatg ccttccagtc
      181 cccggagcac atccgggctt tctgctttca cgatgtcagc aagactttta cgcaaatcgg
      241 agatactttt tgtgattgct tcggaccgcg gtgcgctgcg ggttgcacgc aggagataac
      301 gaagctgccc ttccactttt gcgtgtacca gtttttgcgc aagtgacatt ttgaaagact
      361 catcccgcaa tgcttcgtac tggcgaatac gccgctgtac ctggcccgaa tcaggaacgg
      421 cgcctgttat ataaaacccg gaagcagaca tccaatgtac cgggatccgg ttcagcacac
      481 ataactgcag ggcctgcgtg gtaacctggc cgtatccgtg aataaccatt gactctatct
      541 gcgccaaggg aaatttctgt atttcgccat ccggcgtcgt aacagagatc tgatcacctg
      601 cttttcctac tcgtgctccc ggagtcgtca catgaagtac agtagtttcc tgttttctcg
      661 caaagagcca tttgagaaca agatttgatg agactttgtn nnnnnnnnna gccggagcgg
      721 ttcccagtcc ggatcacagg ccagccgctc ttcttcaggc aggcataccg gcgcgaggga
      781 acagcggata cagaggcgtt cattagctgt tacagcgggc cgcttgagag aagaacggag
      841 ctcgcgggcg cgagcaactg cctgattcac tttgcatctt gcggcatcat ccagaggaag
      901 gcgcaccgta atattttcgg cgtggtaccg tatccggcct tccgttatcg gttttccgag
      961 gtgttcttcc agaagcatgc aataggcata aagctggagg gcatcggagt cccacgcctc
     1021 gtaaggctca cctttccgga ggggagagcg ggggcgtcca cgcttatgtt cgtagacaac
     1081 ccatccgccc tcccgatggc gaacagcatc cagttttcca atgatgccca gagtgtcact
     1141 ggacagctca aattcctgcc gcttttcgcc ttcctgcagc tccaacgttt catgaagttc
     1201 ccggcctgca aagacgcgtt catcagcaac acggatctct tccacttctt cgaggtagaa
     1261 aaggcgctcg caatattcca gtgcatggag agccatgacg cgaattggca gttccagaat
     1321 ttcggtcata gatacatctc cgtccaagcg cgctccggca catccgcccg tggttttccc
     1381 agaatataca gagcggagcg ggtttgggtc atatcgctgc gatgaataca ttgagtcatc
     1441 cgtgtcgtta ccgtgccgtc ttctgtccct gcgtcttcat caagacgctc ccaccagcgg
     1501 atttcctcag gtggagaagt cagatcaagg cggttgggca gaaagttgtt gtctcccaga
     1561 aaaggcagtc catagcgttt tttgacttta cctgagagtc cgtcccgaat agcagcttcg
     1621 aatgattctt ctccatcgac caggatgtag gctttcagat cacagagcag ctcccgcttt
     1681 acaggtttga tgttatactt gctgcctttg cagcggtcag cattttcttt gcggtgcgcg
     1741 ccaaccgggt agttgtgtaa ctgctgatag agcactctga cttcaggggc agcaggagtg
     1801 cccagtgcca gacggcatcc gggcagtccg ctgctgcgaa tcagtgtcat ttccgatttt
     1861 ccgtcgtccc atcgcatctc tatcccggca atattcagga gcaggccata tgccgatgaa
     1921 ggaggcataa accgggcggt aggtctgaaa tatccggccg tgaatatccg aaacaccgca
     1981 aaaggcgctt cgacatagag actgatcatg actgttctcc tgccgcaaag gcctgatcac
     2041 ccaccagatc cagaagcctt gcggcattgc gatccagtac tgtgcctttt tgactcagcg
     2101 tactggaagt cttctcgtcc atatcgcgca ccagtttacc gcccagataa aattcattgc
     2161 cgggaaaatc accggccaga atgccttcca ccacttccgg gaagagatgc gtttgcgtgc
     2221 ggtcggctat ttcaaatcca taggtgttgt agccggcagc caggcagggg gtcaagcgga
     2281 caaccacact tgccggcgcc atttcgaagt aactccgtgc atgattaccg gctacattgt
     2341 tcagctggct gatggcgcgg agcaaggcac gggtccactc cggcttcttt cggcaatctt
     2401 caaggttcaa cgcaaaggga tactgatagg ccgtgaaaac agtttccctg tgaagcaact
     2461 gggaattctg ggcattctgc caggggctgt tcagctgctg gggcgattgc gtaaaaacgg
     2521 tgttgtgtct atatggctcc agagcgacag ccatgttcat gcgcaagatg gagtcccgtt
     2581 taaaggtgaa ggcagcggga tctcttcctt ctttttcttt gatttctttc aaatattttg
     2641 tccgatcctt ggagcctgcc gctaccagcc agcccataaa aaaatcatcc gcccattttt
     2701 cggcatcggg gaaggtggtg aactgaacgg caagctgctc ttcatcttcg agtctttccc
     2761 tgttgcaggg aagtcccagc gcccgcagcc cttcgcggag cgcattgcgt atggcttcgg
     2821 gtgagataat ggcgtattca aagcggtctt tggtgatttt ctgaatgacg gcgcgatttt
     2881 cggcactttc gccacggtag ttggcacttg gggcggcatg ggtcagaaca gtagcaaata
     2941 gattcatggt gatactcctt gactgattca gtcttcttta aaggttgagg ttgaggttat
     3001 gggtttaaag gaccaggaaa cagcagacag ggcaagaagg gaaagagtct tgactttttc
     3061 ccattctgta aacagggcgt tggaaatttc cacataacgg tctttcttaa gattctgggg
     3121 aacagagcac agagttccgg caaagtattc gacaaaatcc tgctcagtac ggccccggat
     3181 cgcaagaaag gcgtccatac atactttctc ttttgcatcg cgataagact caggggtctt
     3241 tacatagccg ttttcatcgc ggttctcctt aaaagagtca taggtgatgc cacttttatc
     3301 ttctgttctg gacatgacat aggtgcgcac cacctcatac acacgctgtg ccagcagttc
     3361 atctttcatc tcttcactca tcggttgtcc tccttttgtg gggttgttgg gttgcagcag
     3421 agaaaacgca taccaggcat ctctcccgaa aaaagaaagc tttcccggag tttcccctgt
     3481 cttttgaata aaaaatttgg cgggcataaa ggccattaca gaaataaatc cccggtacca
     3541 gggcgcatcg gcaagcaaat tagccaggtg ctgcactttg aaaagaccgt tacggaaatt
     3601 ctgtttaaaa cgttcgtagc ggtctaaaat ctggcgggaa gcctcaatac gctgcatacc
     3661 gtgcatctgt acgttgttac caactttttc caggtgatag ctttccacag cggaaacgct
     3721 aaaacggaag gaaccatcag cacgatggcg tgcaagagca cccagatact caagtccgcc
     3781 ttctgcaggc acgtcgattc gggctgcgga tggtcggtac ccagacagct cgggccctaa
     3841 actagcgagc atctccagac agttttcgca aaaatactgc aggtcgagag gctcgggaat
     3901 gaccaggaca aatccgacat catttgtttt tccgtcaaga tctaccgttg ccggacagaa
     3961 aatgcgggaa gccagtggcc aaaaatgaag aagaaggttt tcttcgggta tcccttgaaa
     4021 gccaatcttt tcggcactct ccgcctgagc tccaataaaa atagaactgg caatcggcat
     4081 gagggatggg cctttctttt tagcctgaga tttctcgagt gcctgccaca gcttttcaga
     4141 ttcattttgc tgcactcctt ccagacgtgt tttatagggt atctgggtgg cagggcgacc
     4201 gcggagaata ttccacatga cctctctgta gagtttggtc caggctacct gatcaccatt
     4261 caatgcagaa agaaaagcgg ctttgggctg aataatttcg taaataaacc tcttttctat
     4321 tttggatttc ccgtcttcgt cgatggtctc actttcatct tccctgagag gctcctctcc
     4381 ggctcgttta ttttttaacg caatttcaat attggctccg tcgtagtaat cgtcaaaaac
     4441 actctgaagg ctttcggcag ttactttgat agaagctcca tgtgcactta cttctactac
     4501 ttcggggatg ggagctattt ttctgcgtcg catagattcg agcatcagca gcaatccggc
     4561 aagacctgct ttgtgttgag cagtgggaag atcatgcaag gtgtagcgga gaactaagcc
     4621 ttcagagtgc ttgctatcct gatccgttgc gccaggcggt gctttttcag gaatcattga
     4681 ggtagtcata ggatttcctt tcgtgcttgc gggccgtagc ccaggttggg atcatagttt
     4741 tctgaagatg caatatgaag ccagtcggga aggcgagcgt ctctttttcc ttcctgacgg
     4801 ggaaccggaa gcagcaatcc cgccgatttc ggcgagcgtt gttttcgaag ctcgagataa
     4861 gcaggcacat cgctgtccag gacagcctga accataaaat ctatgccgtc gcgaagactt
     4921 tcctcccggg acagggcatt tacgcccccg ttgataaaag acagccagcg ttccgcttca
     4981 tattgatcgg ggcccagctg cccgagaagt tctgccagat ctttttgtgt aacgagtctt
     5041 tcagtcagtg cggaaacaaa agaggtgaca ccggagagac tctttttatc gtagggaaga
     5101 tcattttcag gtttataaag gaacacctcg ccgatccggt tgctctgagg gagatcacgt
     5161 cggttgcagc ggcccatacg ctgaatcagt gctgtaatcg gtgcatattc cgaaataaga
     5221 acgtcagcat ccaagtccag gctcatttca catacctgcg tggtaacggc acaaacagcc
     5281 tgcttctcgc cctgaaaagc cctgatcacc tgatcgtgct gttttttccg gtcacacaga
     5341 cggaaacggc tgtgatagca cagtgctttc atttcttccg ccagcctgcg gcaacgctcc
     5401 gtggtgttga ccacccaaag aactttgagc ccctgatcca gtgcctgacg agcgtttttc
     5461 agcgcttcat cctcgccagc cagaagcgtc actttatagc gctctttatc ggaacgttcc
     5521 tgtagatccg ggaaatgttc atctccatga tggacaggat caggaaataa acgcaggcct
     5581 aattcttcgg tgagctggca gatgcggtca ccgggaaggc ttgcagtcat acaaagcacc
     5641 ggcagattaa attctttaag aaaggctttc agagcgtcaa acaatccttt gtcatagctg
     5701 tgtatttcat ccagcacaag cacagagtct gccagaagag gaagaagaca gattgaggcg
     5761 tagacgttct gaataaaacc cagaaactgg tctactgttg cagagaagat gcgccgggac
     5821 cagtatccca gagcaaacag acgctcttct gtaagatagg agcgaccgcg gcgatcatct
     5881 gaggcattat caaacatgcc ttgtaaatca aaggttgccg aaccgctaat caaggcggca
     5941 tcgctctccg gcgcccatga aacataatcg cggaatccct cggcagcagt ggcacgggtc
     6001 ggataaagaa aaatgacacg ggaagcaggc ctggcagaca agcgggccgc tatccaacgc
     6061 caggcggcaa gagtttttcc ggagccgcaa ggcgtaatca gcaaggtgcg gtccggaagc
     6121 tccgctgctt tctcctgaaa tccgctccac tgaaaactgg aagaccgctt ttgggttatg
     6181 ctgctaatac ggggggtgat aacactgtca aggatttcct tttcactgag ggattgattc
     6241 ttcgagaata aaggaggaag ccaatcccgc acctctttgt tttccctggt aatacccgag
     6301 cctgcactgt ctgaaatgat tagtgccgca cgcagggccc ggagaaggcg tacttctttc
     6361 ttatcgctgc gaagccgccg atgatatttt tccaagcggt accttaaggc atccgctatt
     6421 ttgtaaacct gacaggtatc cggttgttca taaaaactcc agtgcttcga aagagacaaa
     6481 gaaagcgaag gattaccggg cggcgggggg aaggcttgat ttaaaagaga ctctatgacg
     6541 ggcacattaa tttggaaagt attatgaaca ccaagttttg cgaatctgct tctgtcaaag
     6601 cgcaaatgat gactcatgac aaccgtaatc aataattcca gattaagcga ttgagggaaa
     6661 aagatgcccg cttcaggcat gctcagaatc atgccggaca agaattcgtg atataacgcc
     6721 tgattgagac catcacctgg gctcttgtgc ctaagcatgg cttgaaaggt attgttggct
     6781 tttccgagat cgtgaagcca tgcggctgcc agaccatgct gcagaaaaat gtcatggtct
     6841 tgctgagata attggaaaaa tgtgaaccat tgttcagtta gccagccagt ctttccgtct
     6901 ggaaggaaaa gcgcctgaaa actatctgcc acggcagagg agtgccccat aagcgtctcg
     6961 tgtggtagcg gtgctgtggg acagcgcgtg cttttcgcaa gaataatact tgtagctgcg
     7021 gtcattaatt acgctccgga atgaaaatgc cgcaacccat ctttcttctt ccgcccagtc
     7081 ctcgaatctg caggtcgagc gattcttgtg ct
//
LOCUS       DAOPWB010000150         7056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10643_length_7056_cov_6.474789, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000150 DAOPWB010000000
VERSION     DAOPWB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10643_length_7056_cov_6.474789"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1154..3160
                     /locus_tag="PKN92_11370"
     CDS             1154..3160
                     /locus_tag="PKN92_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HNZ49435.1"
                     /translation="MRPDRDTPLLKRAWFSTGITDFLACSDEHILGSVVQASTFTIET
                     TQRNAWLETFPLLRDALQGLEGQLYFEFEIPRMGRRIDVLAVIEGIIFIFEFKAGATE
                     FQAADWDQAYDYGLDLKNFHEGSHLCPVVPILVATEAPDRLVTLIEHARISGLYETVC
                     TNASQLSCCIHQLRQLIPAVPFDITAWENSRYCPTPTIIEAARALYAGHGVAEISRSD
                     AGAINLQQTSDTLFQIIREAQENKFKALCFVTGVPGAGKTLVGLNIATQQQDDEAPRI
                     FLSGNGPLVAILREALARDMIEREARQGKKIKKGEARTKVKAFIQNVHHFRDNYLMDV
                     QAPAEHVALFDEAQRAWDKEQAANFMKRKRKVSDFNQSEPEFLISVMDRHKDSWAVVV
                     CLVGGGQEINTGEAGIAAWVEAALEHFQDWHLYISPTLKESEYGSGKVLQAIEARKET
                     IHYEFKPELHLGVSMRSFRSEKVSGFVKKVLDCEVLQAKNLLQEINRSYPIRLTRDLN
                     KAKQWLRKQASGNERYGIVVSSAAERLKPYAIDVKTPMDPVNWFLEGKDDVRSSYYLE
                     DVATEFHVQGLELDWACVAWDGDFRYSGKQWQHFAFRGNKWQNINKPERRLYQKNAYR
                     VLLTRARQGMVIYVPAGDPTDPTRRPDFYDQTYAYLRGLGLEEP"
     gene            complement(3253..3702)
                     /locus_tag="PKN92_11375"
     CDS             complement(3253..3702)
                     /locus_tag="PKN92_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49436.1"
                     /translation="MKISVLMSWIGLILMALASSWLTGCPPQSGMLQVINDIDPPLVE
                     LYITTAAAEDWGSNLLDSPLPKDWLCEIEVEPGLWDIFSLNAESGGRMITGVKVDPHK
                     TQSVYVSSMRDLDAEEGKGAGEETGESSSGALAVTGGEDGYACSIMQ"
     gene            complement(3971..4047)
                     /locus_tag="PKN92_11380"
     tRNA            complement(3971..4047)
                     /locus_tag="PKN92_11380"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4011..4013),aa:Val,seq:cac)
     gene            complement(4090..6534)
                     /locus_tag="PKN92_11385"
     CDS             complement(4090..6534)
                     /locus_tag="PKN92_11385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ49437.1"
                     /translation="MRIYHMDRESVWWDEYATHAHLTAPSLTEFLTLSRTLDPLALPL
                     YYTLEYAAFHKISSSLFVLRLLSVLIGIASIYTVWLLGRKSFGTGAGLFAAAMMTLSP
                     VHIYHAQGIRAYGLFTCLAALMIWSFMKLTEEEKRGHWLLHLVVAAALYWTHPFAVLL
                     AAVPGVYLLIQGRKRLFLLIRWAAAQLLLLLPLALWLFSTRFWPRETTESWLALPGFG
                     NVVADIFFDDIAAFQLQFRAGQAASAAGIFRWIPDLLLAALILLLLAVMLRKMLHSKN
                     SKCPLSLHFLWLVLPPLLLLLLSLVLRPCMFPRYTVHCSLALYLLIGAALTQIRAKWK
                     QWLTGTLLILLMAVQWLWLQPGPQRTDWFSAARLLHDQAMPEDLVLVRASLEGELLCH
                     NYRELYGTAPVLPVAEAESSWIIAAQSALALGVQTVAAEEKKTEPGRVLAFLAMDFFV
                     PGPPLDFEFALNAWGIDFQRWFFPAVQEVYAYALTVRPGTEAPRHLKELLEQWEREGL
                     NFEESRADSVKAFGSLALALIGGGRASEASELLRDTQAHMLLDARAYASLTRKMEDPV
                     LRKAAVHALNALYRGYGFRKNGYHMQALQAFQEAGAFDPDNGVVFYELAKEYASEPNQ
                     EAAMNALRQAGRLEEEYASMEAHIQDVMEKGCAFPDAWEAEQAYRRGLLCMSRGNLNQ
                     ADELLKDCLNRCPKHLAALPERAYVLLQQNRLEESAVLLDRYLQICAAPDARALGNRA
                     IIAAITGDDESAHRYIGQAEAADPKFKETFGTFARLFFVEQHFSEALREVERLKEQGI
                     PFPDCVGDYLRKQMAL"
     gene            complement(6657..>7056)
                     /locus_tag="PKN92_11390"
     CDS             complement(6657..>7056)
                     /locus_tag="PKN92_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49438.1"
                     /translation="LRAGRIDPDYCRGGSDALAALVEAARHARITGFTLDGPRGPRYV
                     AKPGIAILAARTGLPIVPNIFAFEKAWRLNSWDRFPIQKPFSRIYCVYGEAVPPPVDE
                     SPDAVETVRMEVETRMNKILETLHKEMDLA"
BASE COUNT         1812 a         1911 c         1794 g         1539 t
ORIGIN      
        1 aagattcctg cctgcgcagg aatgacgagg ggggaggcag gtatgacagg agaaagcagg
       61 aatcttaatc agggcgtggg cgcacttgct tcacaggact gcatgccaca ccaaagtgag
      121 tggacagagt ggacataagt ggacagagtg gactgcgctc ccagccttat taagccgtcg
      181 tcattgctcc cgggaacacc aatcgactta ttggtatgga tagaaccacg aatgaataca
      241 aattatcacc tgaaacttat cttgttcctg caccaaaata ctctgactta aaacataaaa
      301 tccgccagcc tgggaacgcc aatctccgta ttggcagggg cgtttaaggt ctgtgacttt
      361 gtatgtatgt cgtaggacgt atttccgctg caaatccggg tggctgtttt cgccctctcc
      421 cgcttgtgtg ccaatgcgga gattggcgtt cccaggggaa gaattgcctc tccccgtccg
      481 tcattcctgc gaaggcagga atcttaatca ggatatggac gtcttgcctt acatatcacc
      541 ctgatccaca ccaaagactt caagacatac acacacaaaa tgatccttac tttcaaagac
      601 tttcccgcgt agttgacggc aacagcatga cccttataaa tagcatctcg cgttaagatt
      661 cctgcctgcg caggaatgac gaggggggag gcaggtatga caggagaaag caggaatctt
      721 aatcagggcg tgggcgcact tgcttcacag gactgcatgc cacaccaaag tgagtggaca
      781 gagtggacat aagtggacag agtggactgc gctcccagcc ttattaagcc gtcgtcattg
      841 ctcccgggaa caccaatcga cttattggta tggatagaac cacgaatgaa tacaaattat
      901 cacctgaaac ttatcttgtc cctgcaccaa aatactctga cataaaacat aaaatccgcc
      961 agcctgggaa cgccaatctc cgcattggca gggtctttta tggcctgtgc ttattgtgtg
     1021 tatgtcgcag gacatacttc cgctgcggat ttgaatgaaa gcatccgtct acgaaaaaaa
     1081 gcaattcctt ttcccggagc ctttcctata caataagcac tgctgttgtt actgaaaaaa
     1141 atcgaggtgc atcatgaggc cagatagaga cactccccta ttaaaacgtg cgtggttcag
     1201 cacaggcatc acggattttc ttgcgtgttc tgatgaacac atcctaggct ccgttgtaca
     1261 ggcaagcacc ttcaccatcg aaaccacaca gagaaatgcc tggctggaaa cctttccatt
     1321 gctgcgcgat gcccttcagg ggctggaagg gcaactttat ttcgaattcg aaatcccccg
     1381 catgggccgt cgcattgatg tgctggctgt aatcgaaggc attattttta ttttcgaatt
     1441 taaagcaggt gccacagagt ttcaagcagc agactgggat caggcatatg attacgggct
     1501 cgatttaaaa aactttcacg aaggcagcca cctttgcccg gtagttccga tactcgtggc
     1561 aactgaggca ccggatcgtt tggttacact gatcgagcat gcccgcatca gcggacttta
     1621 tgagactgtc tgcaccaatg ccagtcaatt atcgtgctgc atccatcagc tccggcagct
     1681 tattccggcc gttccttttg atattacagc ctgggaaaac tcccgttact gcccaactcc
     1741 cacgattatc gaggcagccc gggctcttta tgcaggccac ggagtagcag aaatttccag
     1801 aagtgatgcc ggcgctatca acctgcagca aacttcggac acactgtttc aaatcatccg
     1861 ggaagcacag gaaaataagt tcaaagcctt atgctttgtg accggagtgc ccggtgcggg
     1921 caaaacactg gttgggctca atattgccac acagcagcag gatgatgaag ctccccgtat
     1981 ttttctttcg ggcaacggcc cgctggttgc cattctccgg gaagcgctcg cacgggatat
     2041 gattgaaaga gaggctcggc agggaaaaaa aattaagaaa ggggaagccc gaacaaaagt
     2101 caaagccttt attcagaacg tccatcattt ccgcgacaac tacctcatgg atgtgcaggc
     2161 tccggcggaa catgtcgccc tttttgatga agcacagcgc gcctgggata aggagcaggc
     2221 tgcgaatttc atgaaacgga aaagaaaagt cagcgacttc aatcaatcag agcccgagtt
     2281 tctgatttcg gtcatggacc ggcataagga cagctgggct gtcgtagttt gcctcgtcgg
     2341 cggcggtcag gaaatcaata cgggtgaggc tggcattgcc gcctgggtgg aagctgcttt
     2401 ggagcatttt caggactggc atttatatat ttcgccaacg ctgaaagaaa gcgaatacgg
     2461 atccgggaaa gtgctgcagg ctattgaagc acgcaaagaa acaatccatt atgaattcaa
     2521 accggaactt catctgggcg tttccatgcg ttctttccgt tcggaaaaag tgtccggctt
     2581 tgttaaaaaa gtactggatt gtgaagtatt acaggctaaa aatcttttgc aggaaattaa
     2641 cagaagttac ccgattcgcc tgacacggga tctgaataaa gcgaagcaat ggcttcgaaa
     2701 acaggcatcc ggaaatgaac gctatggcat tgtggtgtca tccgcggcag agcgactgaa
     2761 accctacgcc atcgatgtca aaacgcctat ggaccccgtc aactggtttc tcgaaggcaa
     2821 agatgatgtg cgttcgtcct attatcttga agacgtggcg acggagtttc atgtgcaggg
     2881 gctggaactg gactgggcct gtgtcgcctg ggacggcgat ttccgttaca gcggaaagca
     2941 gtggcagcac tttgctttca gaggcaataa atggcagaac atcaacaagc ccgagcggcg
     3001 actctaccag aagaatgcct accgggtact cctcacaaga gcacggcagg gcatggtcat
     3061 ttatgtaccc gccggtgatc ccacagatcc gacacggcgg ccggattttt acgatcagac
     3121 ctatgcgtac ctgcgtggcc tcggactgga agagccctga aataccggca ggccattgct
     3181 ctcctgacgc cgcacgccgc agtaaaaccg ggcacttgct cttttttccg gccggacatc
     3241 agacccggct cattattgca tgattgagca ggcgtacccg tcttctcctc ctgtcacagc
     3301 cagagcgccg ctgctgctct cccctgtttc ctcccctgct cccttgcctt cttccgcatc
     3361 gaggtcccgc atggaggaca catagacaga ttgcgtttta tgtggatcaa ctttcacgcc
     3421 tgtaatcata cggcctccgg actcagcatt caatgaaaag atatcccaga ggcccggctc
     3481 cacttcaatc tcgcacagcc agtccttcgg aaggggtgag tcaagaagat tgcttcccca
     3541 atcctcagct gcggcagtcg ttatatacaa ctcaaccagc ggcggatcga tgtcattgat
     3601 cacttgcagc atgccgctct gtggcgggca tccggtaagc cacgatgatg ccaatgccat
     3661 caaaataagc ccgatccacg acatcaatac agatatcttc attgctgttc tccttgctgt
     3721 ctgaagagga caacgattgg aaaagacgcc atgcgatcgg cgctgcatct tttatcagct
     3781 ttatcataac acaggcattt taatctgtaa actgctgttc tacggatggc aagaagagcc
     3841 tttttcgggc cagtcggcag gaaaagaata gagaaaatga ggaggcctta aatatgaact
     3901 ttgcttataa atgaaaagcg gtgcgcgggc cttttaagga acccgcacac cgccgatgtg
     3961 ttctgataaa tggtgggcga tactggactc gaaccagtgg cctctgccgt gtgaagacag
     4021 cgctctaacc agctgagcta atcgcccact gcgtaggaag gatagcacag ccacggcaaa
     4081 ccattcaaat tatagcgcca tctgtttgcg cagatagtca ccgacacagt caggaaaagg
     4141 gataccctgc tctttcagtc gctccacttc acgcagtgct tccgaaaaat gctgctccac
     4201 aaaaaaaaga cgggcgaagg tgccgaaagt ctctttgaat ttcggatcgg ccgcctctgc
     4261 ctgcccgata taccggtggg cactctcgtc atcgcctgtt atggcagcaa taatcgcacg
     4321 gttacccagc gcacgggcat cgggagccgc acatatctga agataacggt cgagcaggac
     4381 agcgctttcc tcaagtctgt tctgctgcag aaggacatac gcccgttccg gtagtgccgc
     4441 caaatgcttc gggcatcggt tgaggcagtc tttcagtaat tcatcggcct ggttcagatt
     4501 tccccggctc atacaaagca gtccccgacg ataggcctgc tccgcttccc aggcatccgg
     4561 aaaagcacat cccttctcca tcacatcctg aatatgcgcc tccatggatg catactcctc
     4621 ttcgagacgg cccgcctgac gcagggcatt catcgccgct tcctgattgg gctccgaagc
     4681 atattccttt gccagctcat aaaaaacaac cccgttgtcc ggatcaaaag cacctgcttc
     4741 ctgaaacgcc tgcaatgcct gcatatgata tccgtttttc ctgaatccgt agccccggta
     4801 caacgcattc agcgcatgca cggccgcttt gcgcagcaca gggtcctcca ttttccgtgt
     4861 aagggatgca taagcccgcg catccagcaa catgtgcgcc tgcgtatcgc gcagcagttc
     4921 ggaagcttcc gaagccctac cgcctccgat cagcgccaga gcaaggctgc cgaatgcttt
     4981 cacgctgtcc gcccggcttt cctcaaaatt gagcccctct ctttcccatt gctccagcaa
     5041 ttctttcaaa tgccgggggg cttcagttcc cggacgcact gtaagagcat atgcatagac
     5101 ttcctgaaca gcagggaaaa accagcgctg aaaatcgatg ccccaggcat tgagtgcaaa
     5161 ttcaaaatcg aggggcggcc cgggcacaaa aaaatccatg gccagaaaag ccagcacacg
     5221 cccgggctct gtcttttttt cttctgctgc cactgtctgt actcccagcg ccagcgcact
     5281 ttgcgccgca ataatccagg agctttccgc ttccgccaca ggaagcaccg gggcagttcc
     5341 atagagctcc cggtaattat ggcagagcag ttcaccttca agagaagctc ttacaagcac
     5401 cagatcttca ggcattgcct ggtcatggag cagccgtgca gcggaaaacc aatcggtacg
     5461 ctgcggcccg ggctgcagcc agagccattg caccgccatc agcagaataa gtaaggtacc
     5521 ggtcagccat tgcttccatt ttgccctgat ctgtgtcaaa gcggctccta tgagaagata
     5581 cagcgccaga ctgcaatgca ccgtatagcg gggaaacatg cagggacgca gcacaagaga
     5641 aagcagcagc aacagcagcg gcggaagtac cagccagaga aaatgtaacg atagagggca
     5701 tttgctattt ttgctgtgaa gcattttacg aagcatcacc gccaacagca gcagaatcag
     5761 cgctgccagc aggagatccg gaatccagcg gaaaatcccc gctgcagatg cagcctgccc
     5821 tgcacggaac tgcagctgaa aagcggcgat gtcgtcaaag aaaatatcgg caacaacatt
     5881 accgaacccg ggtagagcaa gccagctctc tgtggtttcc ctgggccaga accgggtcga
     5941 aaaaagccac agtgccagcg gcagcagcaa cagcaactgc gccgctgccc acctgatcag
     6001 cagaaaaaga cgctttcgcc cctgaatgag caggtacacg cccggcacgg cagccagcag
     6061 taccgcaaaa gggtgcgtcc agtaaagagc ggcagccaca accagatgaa gcagccaatg
     6121 gccccgcttt tcttcttcgg tcaatttcat gaaagaccag atcataagtg cggcaagaca
     6181 ggtgaaaagt ccgtacgccc gtatgccctg cgcatgataa atatgcacgg gcgacagagt
     6241 catcatagcg gcggcaaaaa gacccgctcc ggtaccgaag cttttcctgc cgagcagcca
     6301 gaccgtgtat atggacgcaa tgccgatcag cacactcagc agccggagca caaaaaggct
     6361 tgaactgatt ttatgaaatg cagcatattc cagcgtgtaa taaaggggca gcgccagcgg
     6421 atccagagtt cggctgagtg taagaaattc cgtaagtgac ggcgcggtca gatgggcgtg
     6481 ggtggcatac tcatcccacc acacggactc ccggtccata tgataaattc gcaaagcaaa
     6541 cgccagcacc aggataagga tcagcagggc tgccggcgcg cttcttgcaa atctgtttgc
     6601 agatgggaat atcatgcggt cattttaaaa tgaggcagca gtgtaaagcg aatggatcac
     6661 gccaaatcca tttctttatg aagtgtttcc agtattttat tcatccgggt ttccacttcc
     6721 atccgtaccg tttctaccgc atccggtgat tcgtcaaccg gaggaggcac tgcttcgccg
     6781 tagacacaat agatcctgct gaagggcttc tgaatgggaa aacggtccca ggaattgagc
     6841 cgccatgctt tttcaaaggc aaaaatattg ggtacaatcg gcaatccggt gcgggcggca
     6901 agaatggcaa tgcccggttt ggcaacatag cgggggcctc gggggccatc gagcgtgaag
     6961 ccggtaattc tggcgtgacg cgccgcctcc acaagcgcag cgagggcatc actcccgcct
     7021 ctgcagtagt ccgggtcgat gcgtccggca cgcagg
//
LOCUS       DAOPWB010000151         7006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10725_length_7006_cov_4.910229, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000151 DAOPWB010000000
VERSION     DAOPWB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10725_length_7006_cov_4.910229"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PKN92_11395"
     CDS             complement(<1..972)
                     /locus_tag="PKN92_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49439.1"
                     /translation="MTLTRYIENDLQISAIRHKEYQEYFITAQTGAPDHFQKNVQALA
                     QTLYALNAVPLSAEVFGVPVENRTRWDEALQSRCCPVMWLEGRKDHSSSLQGFQVWAV
                     SGIAAEPVYLNGQAAGMQYTLDGRRWCRLTGLLPQNIGAGRTEQTESVFHAMEEALAQ
                     AGMQFSQVIRTWFYNSEMLQWYDDFNRVRTAFFEKRGIFDQLVPASTGIGCRNGAGAA
                     LTAGLLAVDTPDGFSPPAMVPSPLQCPSIEYGSAFSRAVELSSPSVRRLLISGTASIE
                     PGGKSIHFGDMERQIHLTLCVVQAILRSRDMNWSQVSRAIAYFTDPAD"
     gene            complement(1035..2882)
                     /locus_tag="PKN92_11400"
     CDS             complement(1035..2882)
                     /locus_tag="PKN92_11400"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNZ49440.1"
                     /translation="MKTILSCTFTKGMTRALQLTAALLFLMPAALFGNAAVPPSPTAV
                     VAGKSGTILFVAEKTAGTIAQVDLSSGAVLNRIPLPQPPSALALSPDEKILYAAGGDP
                     EGALFIIHEPFTEVTETFRIGHTPESICISPDGTRAYVACRFNDDIAVVDTAEKTVLS
                     RINAVREPVAAALTPDGSLLFVANLIPKGSSDGDYAAARISIIDTMLQETAAEVFLLN
                     GSSSLRGICVSPDGNYVYVSHILARYHLPTTQLERGWMNTNALSILSVAEKKLINTVL
                     LDDVDAGASNPWGLSCTPDGKCLVVTHAGTHEISVIDRDKLHAKLGSLSDKEGSDTPN
                     NLSFLVDLRTRIKLPGNGPRALWLSDDTAVIANYFSGSIACVPLHKSGTPEIREILLT
                     DGAQTLTEERKGEINFHDAALCFQQWQSCVSCHPDARVDGLNWDLLNDGIGNPKNTRS
                     MLLSHQTPPVMSLGVRAKAEVAVRAGIKFIQFAVRPEEDAEAIDAYLKALTPVPSPRL
                     EKGALSPSAQRGEILFKQAHCAECHPAPLYTDLKLHTLGTTRGMDEGKSVDTPTLIEV
                     WRTAPYLHDGRAATLHELFTIHNSGDRHGKTSSLNEEELADLISYVESL"
     gene            3220..4050
                     /locus_tag="PKN92_11405"
     CDS             3220..4050
                     /locus_tag="PKN92_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ49441.1"
                     /translation="MSQTSLFDLQGKIAVVTGGGGVLGGAIAEGLAAAGATVAVCDLL
                     PDMADACASRIRQSGGTARGYALNVFNSATIVRCSDEVLSEFGGVDILVNAVGGNMKG
                     ATTAPEQSFFDISAEALNKVIDLNLVGGTILPSQIFGKSMIANPGGGSIINISSMNAF
                     RPLTRIPGYSAAKAAVSNFTQWLAVHLAQEYNAALRVNAIAPGFFLTEQNRFLLTDKD
                     SGGLTDRGRTILEHTPLGRFGSPGDLIGAAVWLAGDASRFVTGIVLPVDGGFSAFSGV
                     "
     gene            4112..5014
                     /locus_tag="PKN92_11410"
     CDS             4112..5014
                     /locus_tag="PKN92_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNZ49442.1"
                     /translation="MTDAVAHLKNHEPSHEFLIAIDSDGCAFDTMEIKHKECFIPNII
                     KHWKLQPVSKFARAAAEFVNLYSKWRGANRFPALIKTFDLLADWDAVQARAISLPAIP
                     NLRKWVEEETKLGNPALQAWCDAHSEEEAPDMHIALRWSLEVNRSVTDIVQGGLPPFP
                     FVRTCLDDAVSKADIMVCSQTPGEALIREWEEQGLDKYVFCINGQEVGTKSEHIFHAS
                     EGRYDSRKVLMIGDAFGDLKAARANNALFFPINPGDEEKSWERLHLEGLPRFFSGTFA
                     GDYGAALLAEFEAYLPEMPPWKKA"
     gene            5131..5880
                     /locus_tag="PKN92_11415"
     CDS             5131..5880
                     /locus_tag="PKN92_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNZ49443.1"
                     /translation="MEDMEALARIPSKTLSSHMASVLSRRIIDGEYKEGEKLPTERQL
                     SEEFGVNRHAIREAIKRIEALGLVQIRHGSGIYAQKLNMTAGIEIFNTLLTDNDGSLN
                     IGFLKDVLEFRRHYLMNLTLLALRRRKPRELGKICRIIDNILFNRGKKGSQDESADNY
                     ALFQAIVLASHNHVYYLMYITLSQVIQRLPMILETSFPPFSFTEDDLRKMRHAFEFSN
                     EQEAKEVLESYLQFVERLLKPYLGDEKAKED"
     gene            6060..>7006
                     /locus_tag="PKN92_11420"
     CDS             6060..>7006
                     /locus_tag="PKN92_11420"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNZ49444.1"
                     /translation="MRILVTGGAGFIGSHLCESLIQKQHDVFVLDDLSTGRFENIEHL
                     KEITCVIDSTLNQDVVRDLVKEVDVVYHLAATVGVQLVVNQPIRTIVNNIRGTETILE
                     ETCRYRRRLLITSTSEVYGKGARAVFHEDDDRVIGATHLHRWCYAASKEIDEFLALAY
                     WREKRHPVVLVRLFNTVGPRQTGRYGMVIPTFARQALLGEDLTVYGDGKQTRCFAAVQ
                     DVVPAMVKLMESESICGEVFNIGNNEAVSMEDLAQRIIERTGSSSSIRYVPYDQAYGP
                     GYEDMRHREPCLNKIHAAIGYRPAVSLDEILDAVIADTRR"
BASE COUNT         1593 a         1790 c         1911 g         1712 t
ORIGIN      
        1 atcggcagga tctgtgaaat aggctattgc ccgggatacc tgtgaccagt tcatatcacg
       61 ggagcgcaga atggcctgca ccacacaaag ggtgagatga atctgccgct ccatgtcgcc
      121 gaagtgtatg ctcttacctc ccggttcaat gcttgcagta ccggagatga ggaggcgccg
      181 cacagaaggg gaactgagtt ctacggcgcg actgaaagcg cttccgtatt cgatggaagg
      241 gcattgcagc ggggagggaa ccatggccgg gggagagaag ccgtccggtg tgtcaacggc
      301 aagcagccct gcagtaagcg ctgctccggc gccgtttcgg caaccgatcc ctgtgcttgc
      361 cggaacgagc tgatcaaaaa ttccgcgctt ttcgaaaaaa gcagttctca cgcggttgaa
      421 atcatcgtac cattgcagca tttcgctgtt gtaaaaccag gtgcgaatta cctgggagaa
      481 ttgcattcct gcctgagcga gcgcttcttc catagcgtgg aacacgcttt ccgtctgctc
      541 tgtcctgccg gcaccaatgt tttgaggcag cagcccggtg agcctgcacc atcgccgtcc
      601 gtcgagcgtg tactgcattc ccgccgcctg tccgttgaga taaacaggtt ctgctgcgat
      661 tccggacacc gcccagacct gaaagccctg cagtgagctg ctgtgatcct tccttccttc
      721 gagccacatg acggggcagc accgggactg gagcgcctcg tcccatcgcg tgcgattctc
      781 aacaggcacg ccgaaaactt ctgcggacag cggaacggca ttcagcgcat acagcgtctg
      841 agccagtgcc tgtacgtttt tttgaaaatg atccggtgca ccggtttgcg ccgtgataaa
      901 atattcctga tattccttat gcctgatggc tgaaatctgt aaatcgtttt caatatatct
      961 ggttaaagtc acgaaactct ccctgctatg aaaagcggtc tgcctgagga aaagaaaacg
     1021 cttttcccgt gcgtttacag cgattccaca tagctgatca gatcagcaag ttcttcctca
     1081 ttcagggatg aggttttgcc gtgccggtct ccgctgttat gaatggtaaa aagttcatgg
     1141 agtgttgccg cccgcccgtc atgaagatag ggcgccgtac gccatacttc aatgagtgtc
     1201 ggcgtatcaa cagatttgcc ttcgtccatg ccacgtgtgg tgccaagcgt atgcagtttc
     1261 aaatcagtgt ataaaggagc ggggtggcat tcagcgcagt gtgcctgttt aaacagtatc
     1321 tcaccgcgct gtgctgaagg actcagtgcg cctttttcca gcctgggtga cggcaccggt
     1381 gtcagtgctt tcagatacgc atcgatggct tcagcgtctt cttcgggccg gacggcaaac
     1441 tggatgaatt taatgcctgc ccggacagcc acttccgctt ttgcccgaac gcccagactc
     1501 atgaccggcg gagtctgatg actgagcagc atgcttctcg tgttcttggg attgccgatg
     1561 ccgtcgttca gaagatccca gttcaaaccg tccacacgcg catcgggatg acagctgacg
     1621 cagctctgcc actgctggaa gcaaagtgcc gcatcatgaa aattaatttc ccctttcctt
     1681 tcttcagtca gtgtctgagc gccgtcggtc agaagaattt cgcgaatctc cggcgttcct
     1741 gatttgtgaa ggggaacgca ggcgatgctg ccgctgaaat agttggcgat gacggcagta
     1801 tcatccgaca gccacagggc acgagggcca ttgccgggca atttgattcg ggtgcgcaga
     1861 tcaaccagaa aagaaagatt gttcggtgta tcgctgccct ctttgtcgct caggctgccc
     1921 agctttgcat ggagcttatc ccggtcgatg acgcttattt cgtgggtgcc tgcatgggtg
     1981 accaccaggc atttcccgtc gggcgtgcaa ctgagtcccc agggattgga ggctccggcg
     2041 tccacgtcat ccagcagaac ggtattgatc agttttttct ccgccaccga cagaatactc
     2101 agtgcattgg tattcatcca gccccgctcc agctgggttg taggcaagtg ataccgcgca
     2161 agaatatgac tgacataaac ataattgccg tcgggagaga cgcagatgcc ccgcagactt
     2221 gaactgccgt tgagaaggaa cacttcagcg gcggtctcct gcaacatcgt gtcaatgatg
     2281 gaaatacggg ctgccgcata atcgccgtcc gacgagccct tcggaatcag gtttgccaca
     2341 aaaagcagcg agccgtccgg ggtcagtgcg gcagcaaccg gttcacgtac ggcattgata
     2401 cggctgagga cagttttttc cgccgtgtcc accacagcga tatcatcgtt aaagcgacag
     2461 gccacatagg cgcgcgtacc gtccgggctg atgcatatgc tttccggagt gtgtccgatc
     2521 ctgaaagtct ccgtcacttc ggtaaagggc tcgtgaatga tgaaaagcgc gccttccgga
     2581 tcgcctccgg cagcgtataa gatcttttca tcaggcgaca gggcgagcgc agaggggggc
     2641 tgcggcaggg gaatgcggtt caagactgca ccgctggaca ggtcgacctg tgcaatggtg
     2701 ccggcggtct tttcagcaac aaaaagtatt gtgccgcttt ttcctgcaac caccgctgtc
     2761 ggtgacggcg gcactgcggc atttccgaaa agggcagcag gcataagaaa aagaagcgct
     2821 gctgtcagtt gaagggctct tgtcattcct tttgtgaagg tacagctaag aatggttttc
     2881 ataaggttcc tttcttaacg aatgatagca tttccgggcg cagactttct tctctgtaac
     2941 atttgtctgt ttacctttgt acggtatatt accacgcttt gcctgtgcgt aaaaacccgg
     3001 tgaaatgtac cttccggaga tttggtttaa caatttgttt tttaaataag caatgtgtta
     3061 tagtcccaag ttgtgcaacc gaaatgcgtt ttcagcagtc aaagacgcag aggcggggga
     3121 tatgtttcgc agcgcctttt tgtgtagcat gtgtttttat ctgattttcg cacgcaaaag
     3181 aaaaatacgg gtgattatga tttggagaag taataactga tgagccagac ctcgcttttt
     3241 gacttacagg gaaaaatcgc tgttgtaacc ggcggtggcg gtgttttagg cggtgcaatt
     3301 gctgaggggc tggcagcggc cggtgcaacg gtggcggtgt gcgatcttct tcctgatatg
     3361 gcagatgcct gtgcctcccg tatccgtcaa tccggcggta cagcccgtgg ctatgctctg
     3421 aatgttttta acagtgctac catagtgcgc tgcagtgatg aagtgctgtc ggagtttggc
     3481 ggcgttgata ttcttgtcaa tgccgtgggc ggcaatatga aaggtgctac caccgccccg
     3541 gagcaatctt tctttgatat ttccgccgag gcgcttaaca aagtgattga tctgaatctc
     3601 gttggcggta cgattcttcc ctcgcagatt tttggaaagt caatgatcgc caatccgggc
     3661 ggcggttcca taatcaatat ttcgtctatg aatgcatttc gtcctctgac gcgtattccc
     3721 ggttacagtg ctgccaaagc ggcggtcagc aatttcaccc agtggcttgc cgtacacctg
     3781 gcgcaggagt acaatgccgc ccttcgtgtc aatgccatag ccccgggctt ttttctgacg
     3841 gagcagaacc gctttctcct gacggataag gacagcggcg ggcttaccga tcgcggaaga
     3901 acaattctgg agcatacacc gctggggcgt ttcggcagcc cgggagacct tatcggtgcc
     3961 gccgtgtggc ttgccgggga tgcttcccgg tttgtcacgg gcattgtact gcctgtggac
     4021 ggcgggtttt cagccttttc cggtgtgtaa gtttgcttat gcggcccatt gaaaggtgcg
     4081 gcgttttttc tataaaggag tagatgaatc catgacagat gctgttgctc atttaaaaaa
     4141 ccatgagcct tcccatgagt ttctgatagc catcgacagt gacggctgcg cttttgacac
     4201 catggaaatc aagcacaaag aatgctttat tcccaatatc atcaaacact ggaaattgca
     4261 gcccgtgtcg aaatttgccc gtgcggctgc ggagtttgtc aatctttatt ccaagtggcg
     4321 cggtgccaac cgattccctg ccctgattaa aacctttgat ctgctggcgg actgggatgc
     4381 tgtacaggcg cgcgccatat cgctgcccgc tatcccgaac ctgcgcaaat gggtcgaaga
     4441 ggaaacaaaa ctggggaatc cggcacttca ggcctggtgt gacgcacaca gcgaggaaga
     4501 ggcgccggat atgcatattg ccctgcgctg gagcctcgag gtaaaccgca gcgtcaccga
     4561 tatcgtgcag ggaggcttgc cgcctttccc ctttgtgcgc acgtgtctgg atgatgctgt
     4621 ttccaaagcc gatattatgg tgtgctccca gacacccggt gaagcactga tccgggaatg
     4681 ggaagagcag ggactggaca aatacgtctt ttgtattaat gggcaggaag ttggcaccaa
     4741 atctgagcat atttttcatg catccgaggg aagatatgac agcagaaaag tgttaatgat
     4801 aggagatgct ttcggtgatc tgaaagctgc ccgggcaaat aatgctctgt ttttcccgat
     4861 taatccaggt gacgaagaaa agtcctggga gcgcctgcat ctggaaggtt taccgcgttt
     4921 tttcagcgga acctttgccg gtgactacgg agcggctctg cttgctgaat tcgaagccta
     4981 cctgcccgag atgccgccct ggaaaaaagc ttaaaggtgt ttttcttttg aagagggagt
     5041 gttgcagtca taaaaattgt cggcatttcg tatgctgaat atgcatagaa catagtctga
     5101 tacctttggt acatccctgg aagaagaaaa atggaagaca tggaagccct cgcaagaatc
     5161 cccagtaaaa cactgtccag ccacatggcg agtgtcttaa gcagacgtat tattgacgga
     5221 gaatacaaag aaggggaaaa actgcctacc gaacgccagc tctcggaaga atttggagta
     5281 aaccgccacg ccatacggga agcgatcaag cgtattgaag cgttgggact ggtgcagatc
     5341 cggcacggat ccggcattta cgcccaaaag ctgaatatga ccgcaggtat tgaaattttc
     5401 aacacgctgc ttacggataa cgacggcagc ttgaatatcg ggtttctgaa ggatgtactg
     5461 gagtttcgcc gccactatct gatgaatctg acgctgcttg cgctgcgtcg ccgtaagccg
     5521 cgcgagctcg gaaaaatttg caggatcatc gataatatcc tttttaatcg cgggaaaaaa
     5581 ggctcccagg atgaaagtgc cgacaactac gcactttttc aggccatcgt tcttgcctca
     5641 cacaaccatg tctattacct gatgtacatt acgttgagtc aggtgatcca gcgtctgccc
     5701 atgattctgg aaacctcctt cccccccttc tcctttacgg aagatgacct cagaaaaatg
     5761 aggcatgcct tcgagttctc caacgaacag gaagcaaaag aggtgcttga gtcctatctg
     5821 cagtttgtcg agcgccttct caaaccttat ctgggagatg aaaaggcaaa agaagattga
     5881 cctgcagccg agttactgcg gcgtcatccg gcctttcgct gaaaactccg gcgcccggtt
     5941 tttccggtgc ccccgtctgt gtctgtctct cctgatacct caagttgccg caacgggaag
     6001 gttgcgggta tcatgtgcac aactatcata tccgcctccg tgcggttcag gagaaataca
     6061 tgcgcattct ggttaccggc ggcgccggct ttatcggctc tcatctgtgc gagtctctga
     6121 ttcagaagca acatgatgtc tttgtgcttg acgatctgag cacgggccgt ttcgaaaaca
     6181 ttgagcacct gaaagagata acctgtgtca ttgattccac gctgaatcag gatgtggtca
     6241 gggatctggt gaaagaggtg gatgtggtct accatctcgc tgctaccgtc ggtgtgcagt
     6301 tggtcgttaa tcagcccatc cggaccattg tcaataatat ccgcggcacg gaaaccatcc
     6361 tggaagaaac ctgccgctac cgacgccgtc ttctcattac ctcaacaagc gaagtctacg
     6421 gcaaaggcgc ccgtgctgtt tttcacgaag acgatgaccg ggtgatcggc gcaacgcatc
     6481 ttcaccgctg gtgctatgcc gcttccaaag aaatcgatga atttcttgcc ctggcttact
     6541 ggcgcgaaaa acggcatcct gttgttcttg tacggctttt caacacggtc ggcccccgcc
     6601 agaccggacg ctacggcatg gtcattccga cctttgcccg ccaggcgctg cttggcgaag
     6661 accttacggt gtacggtgac ggaaagcaga cacgctgctt tgcggcggtg caggacgttg
     6721 tccctgccat ggttaaactg atggaatcag aatcaatttg cggggaagta tttaatatag
     6781 ggaataacga agccgtttcc atggaggatc tggcgcagcg gattatcgaa cgtaccggca
     6841 gttcctcttc cattcgctat gtgccttatg atcaggcgta cggtccgggg tacgaagata
     6901 tgcgccatcg cgagccatgc ctgaataaaa tacacgcagc tatcggatac agaccggcgg
     6961 tttctctgga tgaaattctg gatgctgtga tcgcggatac acggcg
//
LOCUS       DAOPWB010000152         6967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10810_length_6967_cov_4.939815, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000152 DAOPWB010000000
VERSION     DAOPWB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10810_length_6967_cov_4.939815"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKN92_11425"
     CDS             <1..592
                     /locus_tag="PKN92_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNZ49445.1"
                     /translation="LQQWVVEATEKGWLQPQGALGLFPCYREEDTVVILDPDEHRQEI
                     ARLPSTVVIGGGNEDLVSGAQYFSEKSDGTFDIIGIQLTTAGAQVESVIDDFKSKDDF
                     ESALFLQGLSNRIAEDMADHVHAKARELVGVAENCGQRWSPGYPGLRDIQLNGVIHKI
                     LDGEKLLGVQVAESGEFLPTGTTGAAVSFHPEARYI"
     gene            652..1227
                     /locus_tag="PKN92_11430"
     CDS             652..1227
                     /locus_tag="PKN92_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNZ49446.1"
                     /translation="MKALAISGSARVNGNTENVLNICLEVLSDAGIPGELIRLAASPV
                     RPCTACGTCFKRKDNTCIQQDDAFHDIYNKMREADILLVGSPVYFGSATADLTALLDR
                     AGYVGRANGNVFSRKLGGPVVVARRAGQNFTLAQLLFWFMIMDMIVPGSSYWNVGFGR
                     AQGEVLEDTEGIETVRHFAENLVWLARKLNA"
     gene            complement(1248..3185)
                     /locus_tag="PKN92_11435"
     CDS             complement(1248..3185)
                     /locus_tag="PKN92_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HNZ49447.1"
                     /translation="MAKNKSCFFLTALFLLAAPMLAYSTEGEEDGTLLDTTADLLNIS
                     SPSFAAAQGAFKLWKQGKASDPVHVWLDALEISESDALQPNEDGSLTMPPDTVWVSES
                     FPVDANTSFLSGAVLGDGSERVSVSLLWLSKQTVLKQDTFLRLELQKDQHFRFNLAEM
                     EVPENADAVRLALCNQDSGQPARWHSILLNAALSYTPEITLTASRLGYEPEWPKTFSV
                     HTNFRASQATFVIKDDTDKKCFQGQLSGAELITGHGGVPWPGYYYQGDFSDFQDEGVF
                     TIEVQCDQCEPVSREIFIRYQVLWNRAFLPALLPFKAMRSEENNPDGHLHLWNETGEN
                     PATDGELLWNLARSWSFLYVRFADSPVLTALEEEVFYGAEKTADYVLGKSPLLSDEPH
                     VQNRFLNALTCIARTGRGSDTILKAACLMMEKQLESSPSDPWTFYAALDLYVATQEER
                     YFEYAAAHVPGITLDRVESMLTYESLSADTVSPFAREQFGKSADILLQSAKNAYDLPQ
                     GAQPHEAGFFLLEKEGAQICRGNTVRVLSAAEIAAQAYRYTADRRYLRFVCSQFNWIW
                     GNNPMDACLVADAGEKEFLIPGAVMHGIGPVNAQTDIPAFPETGEKDWKAYRGFSLNN
                     NASYIVALSHLLRIPTVPPKK"
     gene            3356..5620
                     /locus_tag="PKN92_11440"
     CDS             3356..5620
                     /locus_tag="PKN92_11440"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNZ49448.1"
                     /translation="MLFAFLMPLFSLSAVSVLQQWNFSDPEVCNAWEANTHIENIQCA
                     DGMLQGKTVSWDPFFRCSALAIPARSGQYIRLRMKADVSGRGQLFWTGASDGQYGGFE
                     ERKSTHFQIPVTDGLEDIYLFPFWQTEETIRQLRLDLYDGLQFGIAEIAVLENLQSAP
                     LPEDTAWAFTDSGEAPGWLTLNRASLLVSPPLEIATDKMKWVILEASARRDCALDLFW
                     STDTIAGAQKETVYLRHNDGAARRYYIHPADSRFWQGKLVGLYLSIPPNSSISIRSIS
                     LSPEADGTPELELTYFGFEDAVSRAGRPEKVLAQFTNAGGGEAVIENVRLDTSEGMRV
                     RGSVSSDATPLLRHGETCEIHGTVISDEAGDKTLSLFWNNDTGTADTVLSFAPALKGT
                     AAYVPQPVPVETTRSILAYYFPGWEKNSKWDCIRKTAPVRKPLLGYYDESRVECVDWQ
                     IKWAVENGISCFLVDWYWCQGREHLKHWFEAYRNAKYRDYLQVAIMWANHNPPNTHSR
                     EDWRAVTQEWIDHYFTLDSYYRIKGKPAVFIWNAEGIRHDLGGSRESAAALAESQDMA
                     RAAGFEGIEFVSIQLGAMRHEVDMLKAEGYGGHTSYHEWGNALELASSPALARFSDLV
                     ASGPIYWEKRRALSDGIDYYPLVETGWDSRPWHGNSATVFHGRTVEGFTALLKAARDY
                     CDRHNQNTIILGPVNEWGEGSYIEPCLEYGFSMYEAIRACFGKGDPASWPQNKGPKDM
                     SLGPYDYTASDAAN"
     gene            complement(5640..6941)
                     /locus_tag="PKN92_11445"
     CDS             complement(5640..6941)
                     /locus_tag="PKN92_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ49449.1"
                     /translation="MNKKSETTRRDFMKNAAVLASAAVAGAAVPRNVRAAGSDIIRIG
                     MIGCGSRCTGAAVEALTADPGTRLVAMCDVIPDRIQEKRKRLTQQFQDRIAVDDDHCF
                     AGLGGYRHVIEASDVVLIACAAKFHPWYALQALEAGKHVFVEKPHGIDPLGIQTLKKA
                     AALAEEKKLCLVSGLQSRYHSGYAETIQRIYDGDIGEIISFEENFLRAPYAVTQREPW
                     MSELQWQCSTQYHFTWLSGDDVPQSLVHNLDRVSWVMHDVPPLKCHGLGGRSTMTDHI
                     YGTVFDHHSVVYEYENGVKVFAFCRTTDNCFNDNSSIVYGTKGKASLLGTEIWGDKAW
                     KWKGSCNPYQVEHDVLFRAIRSGEPVNNGAYMAQSTLMGIMGQISCYTGEEIYWDKIS
                     QSDFSFGPRPEACNDDMEPPVLPGEDGSYPTFRPGITKLLG"
BASE COUNT         1686 a         1838 c         1826 g         1617 t
ORIGIN      
        1 cctgcagcag tgggtagtgg aagcgacaga aaaaggatgg ctgcagcctc agggggcact
       61 gggactcttt ccctgttacc gtgaggaaga tacggttgtt attctcgatc cggacgagca
      121 caggcaagaa atagcccgcc tgccttctac cgttgtgatt ggcggcggca atgaagacct
      181 cgtttccggt gcgcagtatt tttccgaaaa aagcgacggc acttttgaca tcatcggcat
      241 tcagctgact acagccggcg cacaggtgga atctgtgatc gacgacttca aaagcaaaga
      301 tgattttgaa tcggcgctat ttctgcaggg attatccaac cgcattgctg aagacatggc
      361 agatcatgtt catgcgaaag ccagagagct ggtcggtgtt gccgaaaact gcgggcagcg
      421 ctggtcaccg ggctatcccg gcctgcgcga catccagttg aatggtgtca tacacaaaat
      481 actggacggt gaaaaactgt tgggtgtgca ggttgcggaa tccggcgaat ttcttccgac
      541 aggcacaacc ggcgctgcgg tctccttcca cccggaggcg cgctatatct gaagcgataa
      601 cggtcggctc gtggtaaaat aatgaagaaa acctttcgag gaggtcatac catgaaagca
      661 ctggcaatca gcggaagcgc ccgagtcaat ggaaacacgg aaaacgtcct gaatatctgt
      721 cttgaagtgc tttccgatgc aggcatcccc ggggaactga tccgcctggc ggcgagcccc
      781 gtgcggccct gcactgcatg cggcacctgc ttcaaacgga aagacaacac ctgcattcag
      841 caggacgacg cctttcatga tatttacaac aaaatgcggg aagcagatat cctgctcgtc
      901 ggatctcctg tttatttcgg atctgccaca gccgatctga ccgcactgct ggatcgtgcg
      961 ggctatgtag gacgtgccaa cggaaatgtc ttttcccgaa aattgggagg ccctgttgtg
     1021 gttgcacgcc gtgccgggca aaatttcacc ctcgctcagc ttctcttctg gttcatgatc
     1081 atggatatga tcgttcccgg cagctcttac tggaatgttg gcttcggtcg tgctcagggt
     1141 gaagtgctgg aagacacgga aggaatcgaa acagtgcgcc actttgcgga gaatcttgtc
     1201 tggcttgccc ggaaactcaa cgcctgatct tcttttgtat cctcagccta ttttttgggt
     1261 ggtacagtcg gaattcgaag caggtgagac agggcaacaa tataagaggc gttgttattc
     1321 agtgaaaagc cccggtacgc cttccagtct ttttcacccg tttccgggaa tgcgggtatg
     1381 tccgtctgtg cattcacagg acctatgcca tgcatcacag cgcccgggat cagaaactct
     1441 ttttctcccg catctgccac aagacaggca tccatcgggt tattgcccca gatccagtta
     1501 aactggctgc acacaaaccg cagatatcgc ctgtctgctg tataacggta tgcctgggct
     1561 gctatttccg cagcagaaag cacgcggact gtgttgcccc gacagatctg cgcaccttct
     1621 ttctcaagga gaaaaaaacc ggcctcgtgc ggctgtgccc cctggggcag atcataagca
     1681 tttttggcgc tttgcagcaa tatatccgct gattttccaa actgctcccg tgcgaaaggc
     1741 gacacggtat cagcggacag gctttcatag gtgagcatgg attccacacg gtccagagta
     1801 atgccgggca catgggcggc ggcgtactca aaatagcgtt cctcctgtgt ggcgacgtag
     1861 agatccaggg cagcataaaa tgtccatgga tctgaaggag aagattccag ttgcttttcc
     1921 atcatgaggc aagcagcctt cagaatagta tcgctccccc ttcccgtgcg ggcaatacag
     1981 gttaatgcat ttaaaaagcg attttgcaca tgaggttcgt cagagagtaa agggcttttc
     2041 ccgagcacat aatcggctgt tttttcggct ccataaaata cttcttcttc cagtgcggta
     2101 agaacggggc tgtctgcaaa gcgtacatac agaaagctcc aggaacgtgc gagattccat
     2161 agaagctctc catcagtcgc cggattctct ccggtttcat tccagagatg caggtgtccg
     2221 tcgggattat tttcttcact ccgcatcgct ttaaagggca gaagtgcagg taaaaaagca
     2281 cggttccaca gaacctgata tcggataaag atttccctgg ataccggctc acactgatca
     2341 cactgcacct ctatggtaaa tacgccttcg tcctgaaagt ctgaaaaatc tccctgatag
     2401 taataaccgg gccagggaac cccgccatgt ccggttataa gttcagcacc ggacagctgc
     2461 ccctgaaaac attttttatc cgtatcgtct ttgatcacaa aagttgcctg cgaagcacgg
     2521 aaattggtgt gcacggaaaa ggttttcggc cattcaggtt cgtaaccgag gcggcttgcc
     2581 gtcagggtaa tttcaggagt ataggaaagt gctgcgttca gaagaatgga gtgccagcgg
     2641 gcgggctgcc cggaatcctg attgcagagg gcaagccgaa cggcatcggc gttttccgga
     2701 acctccatct cggcaagatt gaaccggaag tgctgatctt tctgaagttc taaacgaaga
     2761 aaagtatcct gtttgagaac tgtctgcttc gagagccata aaaggcttac tgacacacgc
     2821 tctgagccat cccccagcac tgcaccggac aaaaaggagg tattggcgtc tacgggaaaa
     2881 ctttcagaca cccagaccgt atctggcggc attgttaatg aaccgtcttc attgggctgc
     2941 aacgcatcgg attcggatat ttccaaggca tccagccaga catgcaccgg atcggaagcc
     3001 ttcccctgct tccacagttt aaatgctccc tgagctgccg caaaagaggg actgctgata
     3061 ttcaacaaat ctgcagttgt atcaagaagg gtaccgtctt cctctccttc ggtggaatag
     3121 gcaagcatcg gggctgctaa aagaaaaagt gcggtcagaa aaaagcagga tttatttttc
     3181 gccatttcga aagaactccg gaattatctg taataagagg aaagcggtat gcgaaacttg
     3241 caccagcctt ttggaagata gtgtttactt gtggataata acatgatata agacaaactc
     3301 ccaagcacca ccatgcctct ctgtccccca ggcatcttac aggagatata ccgccatgct
     3361 gttcgcgttt ctcatgcctc ttttcagcct gtccgccgtt tcagtgctgc agcaatggaa
     3421 cttttcagat cctgaagtct gcaatgcctg ggaggcgaat acgcatattg aaaacattca
     3481 gtgtgccgac ggcatgctgc aggggaaaac agtcagctgg gatcctttct tccgttgttc
     3541 cgcccttgcc attcctgcac gatcggggca atatatccgt ctgcgcatga aagcggatgt
     3601 ttccggcaga ggacaacttt tctggacagg tgcttccgat gggcaatacg ggggattcga
     3661 agaaaggaaa tcaacccatt ttcagatacc cgtcaccgac ggactggaag acatttatct
     3721 gttccctttc tggcagacag aagaaactat cagacaatta cggcttgacc tgtatgacgg
     3781 gctgcagttc ggcattgctg aaattgctgt tctggaaaac cttcaaagtg ccccccttcc
     3841 cgaagataca gcgtgggctt ttactgattc gggtgaagcc cccggctggc tcacactgaa
     3901 ccgcgcctcc cttctggtga gcccgcctct ggagattgcg acagacaaaa tgaaatgggt
     3961 gattctggag gcatccgccc gcagagactg cgcactggat cttttctggt caaccgatac
     4021 aatcgcaggc gctcagaaag aaaccgtcta tcttcgacac aatgacggag ctgcccgccg
     4081 gtattatatt catcctgcag acagcaggtt ctggcaggga aaacttgtcg ggctctatct
     4141 gagcatcccg cccaacagca gcatctcgat acgaagcatc tccctgtcgc cggaagcaga
     4201 cgggactcct gaactggaac tgacttattt cggctttgaa gatgccgtga gccgcgccgg
     4261 aagaccggaa aaagttctag ctcaattcac caatgcaggc ggcggagaag cagtcatcga
     4321 aaatgtccgg ctggacacgt ctgaagggat gcgtgtgcgg ggatctgtaa gcagtgacgc
     4381 cacgccgctg ctgcgccatg gcgagacctg cgagatacat ggcacagtaa tcagcgatga
     4441 agccggcgat aaaacactgt ctctgttctg gaacaacgat actggcacag cagacacagt
     4501 actttctttt gcccccgccc tgaagggaac cgctgcgtat gtgcctcagc ctgtccccgt
     4561 tgaaacaacg cgatccattc tggcttacta ttttcccgga tgggaaaaaa actcgaaatg
     4621 ggactgtatc agaaaaacag ccccagtgcg caaaccgctt ctgggctact acgatgaatc
     4681 ccgggttgaa tgcgtcgact ggcagatcaa gtgggcggtt gaaaacggta tctcctgctt
     4741 tctggttgac tggtactggt gccagggccg cgagcacctg aagcattggt ttgaggcgta
     4801 tagaaacgca aaatacagag attacctgca ggttgccatc atgtgggcaa accacaatcc
     4861 tcccaatacg cacagccggg aagactggcg ggcagtgaca caggaatgga ttgatcatta
     4921 cttcacactg gacagttatt atcggatcaa aggaaaaccg gctgtgttca tttggaacgc
     4981 ggaggggatc cgccacgatc tgggcggaag cagagaatct gctgctgcat tggcggaatc
     5041 gcaggatatg gcacgggcag ccggatttga aggaatcgaa tttgtttcga ttcaattggg
     5101 tgcaatgcgg cacgaagtgg atatgctgaa agcagaagga tatggcggac ataccagtta
     5161 tcatgaatgg ggcaacgcgc tggaacttgc gtcttcaccc gctctggcac gcttttccga
     5221 tctggtagca tcagggccga tttactggga aaagcgtcgg gcactttccg acggtataga
     5281 ctattatccg ctggtggaaa caggatggga ttcccgtccc tggcatggta acagcgccac
     5341 ggttttccat gggcgtaccg tggaaggctt cacagcgctg ctgaaagccg cccgggatta
     5401 ttgcgacaga cacaaccaga acacgattat tctgggacct gtcaatgaat ggggcgaagg
     5461 cagctacatt gagccctgcc tggaatacgg cttttccatg tatgaagcga tccgggcatg
     5521 tttcggcaaa ggcgatccgg cttcctggcc tcaaaacaaa ggccccaaag acatgtctct
     5581 gggaccttat gactatactg cttccgatgc ggcgaattga ttcttctgca ccgttttatt
     5641 tagccgagca gttttgtgat gccgggccgg aaggtcgggt aagaaccgtc ttctcccggc
     5701 agcaccggcg gctccatgtc atcgttacac gcctccggtc ggggtccgaa ggaaaaatcg
     5761 gactggctga ttttatccca gtaaatttct tcgcctgtat agcaggatat ctgacccatg
     5821 atccccatca gcgtgctttg tgccatgtag gcaccgttgt tcacgggttc accggagcgg
     5881 atggcacgga agagaacgtc atgctccacc tgataaggat tgcaggagcc cttccatttc
     5941 cacgccttgt caccccagat ttcagtgccc aacagagagg ccttgccttt ggtcccgtaa
     6001 acgatgcttg agttatcgtt gaagcagtta tccgtcgtac gacagaaggc aaaaaccttg
     6061 actccatttt catattcata caccacggag tggtgatcga atacggtacc gtaaatgtga
     6121 tctgtcatcg tggaacgtcc gccgagacca tggcatttga gcggcggcac atcgtgcatc
     6181 acccaggata ccctgtcaag gttatgcacc agggattgcg gcacatcatc tccggacaac
     6241 caggtaaaat gatactgggt actgcactgc cactggagtt ccgacatcca gggctcgcgc
     6301 tgagtgaccg cataaggcgc acggaggaag ttctcctcaa acgagatgat ttctccgata
     6361 tccccgtcat aaatccgttg aatcgtttcc gcatagccgc tgtgataccg gctctgcaga
     6421 ccggaaacaa gacacaattt tttctcttca gccagtgccg ccgctttttt cagcgtctgg
     6481 atgcccagtg gatcgatgcc gtgcggcttc tccacaaaca catgcttgcc cgcctccagc
     6541 gcctgcagag cataccaggg atgaaattta gcggcgcagg caatcagcac tacatcggaa
     6601 gcctcaatga catggcggta tccgccaagc ccggcaaaac aatggtcatc gtccacggca
     6661 atcctgtcct gaaactgctg cgtcagccgt ttccgtttct cctgtatccg atcaggaatc
     6721 acatcgcaca tggctaccag acgggttccg ggatcagcag tcagcgcttc cacggccgca
     6781 cctgtgcagc ggcttccgca tccgatcatg cctattctga taatgtcgct tcctgctgcc
     6841 cggacattgc gcggaacagc cgcaccggca acagccgcag acgcaagcac cgccgcgttt
     6901 ttcataaagt cgcgccgtgt cgtttcagat ttcttattca tcatttaccc ttcttcggtt
     6961 ttgcttt
//
LOCUS       DAOPWB010000153         6966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10812_length_6966_cov_5.739835, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000153 DAOPWB010000000
VERSION     DAOPWB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10812_length_6966_cov_5.739835"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKN92_11450"
     CDS             <1..220
                     /locus_tag="PKN92_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49450.1"
                     /translation="QRATVDCGYRQVPACASCHLRHICDGFHGDYTEFFGVEEARPVT
                     DVPETEDPRIFIKEQEKHVLAEDCDWAL"
     gene            274..1485
                     /locus_tag="PKN92_11455"
     CDS             274..1485
                     /locus_tag="PKN92_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNZ49451.1"
                     /translation="MQAVIMAAGKSTRTWPLTLTRPKPLLPVMGMPLLEHQLRSLQGI
                     ADEVIIIIGFMGDMIQQHYGGQYGTIRLRYVEQKEQRGTGHAVLQTADAVKGPFLVLN
                     GDDMYDPADLQKLAFQPDAALAREVESPERFGIFEIDGDNRVLGLEEKPARPRSNLAN
                     IGAYSFTPDIFGLLEKVAPSPRGEIEVTDAVHERALKDVFRVIHARGYYLSIGYPWHL
                     LEANSYWLDHFLQDCRQGEISPLAEVNGKVHIGEGTGIRAGVVIDGPAFIGKNCIIGP
                     NCWIRSGTTIGNGCRIGQGTEIKNSVLFDGVFAAHRNYIGDSIIGEGCNLGAGTTTAN
                     ARHDEGTISSLMGGVLQDTGRLRLGAALGDKVHTGIHTAIYPGRKLWPGCHTLPGSII
                     RKDVTENTPED"
     gene            complement(1604..2554)
                     /locus_tag="PKN92_11460"
     CDS             complement(1604..2554)
                     /locus_tag="PKN92_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNZ49452.1"
                     /translation="MKLVEKKGFTLIELLTVIAIIAILAGLTMTVGPRMIERAKLRRL
                     DSAMREVATALTAYYVDHTTYPPAYGYVNFEKRDSNPPADPSEDSGFYNLQPYMFRMK
                     YHGNEELSDEFSSSYDTDKDGRLSLLEYSPLGVRQPNGTYQMDFSLPRYTGAVTPEFY
                     PEVERQLQATRRPFIYIPVNLQQFSRVQRYWLNHADAFASRWNPSDPDFPQLSFPPKH
                     YDAFVLISLGPAGNSFGLLPDPIGTEPARDVYHITALRAYYMATRDLNANGKLDFDFR
                     ARTQQQEAGLTYSLGSQPMTNELPSATMPNGFGPYIFVSK"
     gene            complement(2654..2998)
                     /locus_tag="PKN92_11465"
     CDS             complement(2654..2998)
                     /locus_tag="PKN92_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49453.1"
                     /translation="MSEYFCCKCGTPWESAHTQPGFKEYCIKCSAWLHSCAHCRFHRK
                     TAPNQCEIPGTEAVRDRNGMNYCDAFAFREGRVETPDLEKQEEARKKLASLFQEEETE
                     RKPLSFDDLFLE"
     gene            complement(3012..4043)
                     /locus_tag="PKN92_11470"
     CDS             complement(3012..4043)
                     /locus_tag="PKN92_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49454.1"
                     /translation="MRRANNFRPGMCLLTVCFLTAFLGVYAGAAEKKGAAPKKEKAAE
                     AAKETSGDTQKNTTEGTTFQPLRIVGGIPDTLPIPGDARPYAESRYAKEIDRALAFMV
                     FKCRESGRWIWYVLLDYLQRQYDLPERYSFEFTASSVVMTEENDLRDRLLRMSDPAFM
                     LPLEKIEESSGAEYFLQRALYCKDYPIEDELVAQMADFVADESNYVVSPVAFAAVIMR
                     QGQCVANTRNYMRLRGTLGVLYRERLTKEGIRNAFSAEALAMLKLLKYEKMLQENWLP
                     EAVSYQNEDGGWADVFRRSNPGSSAPRATLFMLWAMLQDAMPDVEEVPLLKPGAVAEG
                     EAAEVIKSP"
     gene            4361..5695
                     /locus_tag="PKN92_11475"
     CDS             4361..5695
                     /locus_tag="PKN92_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNZ49455.1"
                     /translation="MMSEFWHDAGCVFHPVSAPDYLPETQLKQLQLHRLRAVVQRSYD
                     HVALFRERMQGKGITPEDIRQLEDIQHLPFTVKTDLRDTYPFGLFASPMNEIVRMHAS
                     SGTTGKPIVVAYTQEDLRVWSSVMVRCFAAAGLDRGDIIQNAYGYGLFTGGLGAHYGA
                     EALGATVIPISGGNTDRQIMLLKDFGATAICCTPSYFLRILERAPELGVNLRDLPLKA
                     GIFGAEPWTDEMRKRIEPEANIKAYDIYGLSEIIGPGVANECSAQDGLHIFEDHFYPE
                     IVDPETLQPVPDGEEGELVLTTLSKQAMPMIRYRTRDITTLYKEKCSCGRTVRRMRRI
                     ARRSDDMFIIRGVNVFPSQIEEALLAVEGTAPHYQIVLTNDGTMDDVEVQVEVSPEAF
                     SDKVSVMEGLRKKLSASIERVVNIRVNVRLVEPNKIARSEGKAKRLIDNRKK"
     gene            5788..6228
                     /locus_tag="PKN92_11480"
     CDS             5788..6228
                     /locus_tag="PKN92_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNZ49456.1"
                     /translation="MKMHQLSMFLENKPGRLSEPCRLLANAGINILTLSVADTQQFGI
                     LRLIVPDWEAAKGVLEKAGFVVNVTEVVATEVVDQPGGLATILEIIEEVGLNIEYMYA
                     FTFRCNDKAVIVFRFDNNDIAVTALQAKGISVVGGVELYERARQ"
     gene            6313..>6966
                     /locus_tag="PKN92_11485"
     CDS             6313..>6966
                     /locus_tag="PKN92_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNZ49457.1"
                     /translation="MNTFAIDNQILDKEETMPRTELQALQLKRLRETVARAMNVPFYK
                     KEFAERGITPDSIQTLSDVRRLPFTTKEDMRSNYPLGLCAVPRTELARIHGSSGTTGM
                     PTFVGYTHQDLKTWARLCARFLVAGGLRPGHTAQIAFGYGLFTGGFGLHYGVEEVGAA
                     VLPVSSGNTTRQVQLMRDLQPDALICTPSYALNIAEVARGMGIDPRTLSLRFAHFGGE
                     "
BASE COUNT         1618 a         1872 c         1890 g         1586 t
ORIGIN      
        1 gcagcgcgcc actgtggact gtggctacag gcaagttccc gcgtgtgcaa gctgccacct
       61 tcgccatatc tgtgacggat ttcacggcga ttatacagag tttttcggtg tggaggaggc
      121 acggcctgtc actgatgtgc ctgaaacgga agatccccgt attttcatta aagaacagga
      181 aaagcacgtg cttgccgaag attgcgactg ggcgctgtaa cgccgccggc agcagtaaaa
      241 cgtagtagac gaaaccggga ggagatgcct ttcatgcagg ctgtcattat ggcggcagga
      301 aaatcaacgc gcacctggcc gcttaccctt acaagaccca aaccgctgct gcctgtcatg
      361 ggcatgccac ttcttgagca tcagctccgc tcgctgcagg gcatcgccga tgaggtcatc
      421 attatcatcg gctttatggg agacatgata cagcagcact atggcgggca gtacggcacc
      481 atacggctgc gttatgtgga gcagaaggaa cagcgcggca ccggccatgc cgtcctgcag
      541 accgcggatg ctgtaaaagg cccttttctc gtgctaaacg gcgatgatat gtacgatccc
      601 gccgatttgc agaaactggc ttttcagcct gatgcggcgc tggcgcggga agtggaatcg
      661 cccgaacgct tcggcatttt cgagatagac ggggataatc gggtgctggg tctggaagaa
      721 aagcccgcca gaccccgctc aaatctcgcc aatatcggcg cttattcctt cacgccggat
      781 attttcgggc ttctggaaaa ggtcgcaccc tcgccccgcg gcgaaattga agtgaccgat
      841 gcagttcatg aacgggcgct gaaagatgta ttccgggtga tccatgcccg gggatattat
      901 ctttccatcg gctatccctg gcatctgctg gaagcaaaca gctactggct ggatcacttt
      961 ctgcaggact gcaggcaggg cgaaatcagc cctctggcag aggtgaacgg caaagtacac
     1021 atcggcgaag gcacaggtat ccgtgcaggt gtcgttattg acggacctgc ttttatcgga
     1081 aagaactgca taatcggtcc caactgctgg atccgatccg gcacaaccat cggcaacggc
     1141 tgccgaatcg gccagggaac ggaaattaaa aactccgtcc ttttcgatgg cgtttttgca
     1201 gcgcaccgga attatattgg cgacagcatt atcggtgaag gctgtaatct gggtgccgga
     1261 acgaccactg ccaatgcgcg ccacgacgaa ggtaccattt cttcgctcat gggcggcgtt
     1321 ctgcaggata cgggccggct tcgtctgggt gccgcacttg gcgacaaggt gcatacgggt
     1381 atacacacag ccatttatcc cgggcgtaaa ttgtggcccg gttgccacac ccttccgggc
     1441 agtattatcc ggaaggatgt gacggaaaat actccggagg actgaaaact cccccggata
     1501 tacggcgaac gcccatgagc actgaagtct catgggcgtt cttttacgtc attcctttat
     1561 tttcactctg ctgtcccgct agcaggcaca gcagacagga ggcttatttg ctcacgaaaa
     1621 tgtaggggcc gaacccgttg ggcatggtgg cggaaggcag ttcgttggtc atcggctggc
     1681 ttcccagtga ataggtcagc cctgcttcct gctgctgcgt gcgcgcacgg aagtcaaagt
     1741 ccagttttcc gttcgcattg aggtcgcgcg ttgccatgta ataggcgcgc agagcggtaa
     1801 tgtgatagac atcacgggcc ggttccgttc cgataggatc cggcaggagt ccgaaagaat
     1861 tgccggcagg tcccaggctg atgagcacga aagcatcata atgtttaggc gggaagctca
     1921 gttgcggaaa atccgggtcg ctgggattcc agcgggaggc aaaagcgtca gcatggttca
     1981 gccagtaacg ctgcacacgg ctgaactgct ggagattcac cgggatatag ataaaaggac
     2041 gccgagtggc ctgcagttgg cgctccactt ccggataaaa ctcaggggta acagcaccgg
     2101 tgtagcgggg gagtgaaaaa tccatctggt acgtgccgtt gggctggcgt acaccaagag
     2161 gtgaatattc gagcagactc aatctgccgt ccttgtcggt atcgtaggaa ctggagaact
     2221 catcgctaag ctcttcattt ccatggtatt tcatgcggaa catgtagggc tgcagattat
     2281 agaaaccgga gtcttccgag gggtctgcag gcggattgct gtctctcttt tcaaaattca
     2341 cataaccata agcgggcgga taagtggtat gatccacata atatgccgtg agcgctgttg
     2401 ccacctcgcg catagcactg tccagacggc gcagttttgc acgctctatc atccggggcc
     2461 cgacagtcat cgtcaatcct gccagaattg caataattgc aatcactgtc agcaattcaa
     2521 ttaatgtaaa gccttttttc tcaaccaatt tcatgttaat ctcctctggg ggatcttccc
     2581 tgtctctctg gtgattatac cacaaaaacc agaggcgggt attttttcac ctgatccgcg
     2641 tgcagcgggc atcctattcc aaaaacaaat catcaaaaga cagcggtttt cgctcggttt
     2701 cttcttcctg aaaaagagac gccagtttct tccgcgcttc ctcctgcttc tccagatccg
     2761 gtgtttcgac acgcccttca cgaaatgcga aggcgtcgca ataattcatc ccgttgcggt
     2821 cccggacggc ttccgtaccc ggaatttcac actgattggg tgctgttttc cggtgaaagc
     2881 ggcaatgggc acagctgtga agccaggcgc tgcatttgat gcagtattct ttgaagcccg
     2941 gctgagtgtg cgcactttcc cagggcgttc cgcatttaca acagaaatat tcagacattg
     3001 ccgctgctgc ctcaagggct ttttattact tccgctgctt cgccttcggc taccgctccg
     3061 ggcttcagca acgggacttc ctccacatcg ggcatggcat cctgcagcat agcccagagc
     3121 ataaagaggg tggcgcgggg agcactgctt ccggggttgc ttctcctgaa tacatctgcc
     3181 cagccgccat cttcattctg gtaggaaact gcttcaggca gccaattttc ctgaagcatc
     3241 ttttcatatt tcaggagctt cagcatggca agtgcttcgg cagaaaaggc attccgaata
     3301 ccttcttttg tcaggcgttc ccgataaaga acgcccagcg tgccccgaag ccgcatataa
     3361 ttccgggtat ttgccacaca ctgaccctga cgcatgataa cggctgcaaa cgcaaccgga
     3421 ctcacgacat agttgctttc gtcggcgaca aaatcagcca tctgagcgac cagttcgtct
     3481 tcaatcggat aatccttgca gtaaagggca cgctgaagga aatattccgc cccgctgctt
     3541 tcctctatct tttccagagg aagcatgaat gcaggatcgg acatgcgcag caatctgtcg
     3601 cgcagatcgt tttcttctgt catcactaca ctggaagcgg tgaactcaaa gctgtaacgt
     3661 tccggcagat catattgccg ctgcagataa tccaacagga cgtaccatat ccagcgaccc
     3721 gattcgcggc atttaaaaac catgaacgcc agcgcccggt caatttcttt tgcatagcgg
     3781 ctttcggcat aaggccgggc atcgccgggg atcggaagcg tatcaggtat acctcccaca
     3841 atgcgcagtg gctgaaaagt ggtgccttcc gtcgtgttct tctgtgtatc tccggaagtc
     3901 tctttggcgg cctcggctgc tttttctttt ttcggagcag cccctttctt ttctgccgca
     3961 ccggcgtaca ctccgagaaa agcggtcaga aaacacactg tcagcagaca cattcccggt
     4021 ctgaaattat ttgcccttct catctttcca tatcctttct tctatcgaaa atacggttta
     4081 tcgccgccac cagggcacct gtatacaaat tgaatccatg cagcattata acaaagacgc
     4141 atcgaccagg agcagtttta cgcccaatag ccctccggca cgtgcaaagg catcccgccg
     4201 ttgtttaaac tggcggcact tgcatctgaa gaatgctgca gcggatttgt accaatcctg
     4261 attctcgtac tatacttacc gtcatgcaga tacatggaac cgggcgaaga catgacagcc
     4321 ccggagcgat attctcagat tctagggtaa aaggaagcag attatgagcg aattctggca
     4381 tgacgcaggc tgcgtttttc acccggtcag tgcaccggat tacctccctg aaacacaatt
     4441 gaaacagttg cagctccatc ggcttcgtgc tgttgtacag aggagctatg atcatgtggc
     4501 actttttcgg gagcggatgc agggaaaagg gatcactcct gaagatatcc ggcagctgga
     4561 ggatatccaa caccttccct tcacggtaaa aacagatctg cgggatacct accctttcgg
     4621 tctttttgcc agtccgatga acgaaattgt gcgcatgcat gcttccagcg gtacaacggg
     4681 caagcccatt gttgtcgcct atacacaaga agatctccgg gtctggtcga gcgtgatggt
     4741 tcgctgtttt gccgcggcgg gactcgaccg gggcgacatt atccagaacg catacggcta
     4801 cggccttttc acgggcggtc tgggtgcaca ctatggtgcc gaagcactgg gcgccacagt
     4861 aatccccatc tccggcggca atacggatcg ccagatcatg ctgctcaagg acttcggcgc
     4921 cacagcgatc tgctgtacgc ccagctattt tctccgcata cttgagcgtg ctcccgaact
     4981 gggtgtaaac ctccgggacc ttccgctgaa agcgggtatt ttcggtgcgg agccctggac
     5041 agatgagatg cgcaagcgca ttgaacctga agccaatatt aaggcgtatg atatttacgg
     5101 gctctccgag atcatcggac cgggcgttgc caatgaatgt tcggctcagg acggcctgca
     5161 tatttttgaa gaccattttt atccggagat cgtggatcct gagacactcc agcctgtgcc
     5221 ggacggcgaa gaaggcgaac tggtgctgac aaccttgagc aagcaggcga tgccgatgat
     5281 ccggtaccgt acaagagata tcaccacgct ttacaaagaa aaatgcagct gcgggcgcac
     5341 agttcgccgg atgcgccgta ttgcacggcg cagcgacgat atgtttatca tacggggcgt
     5401 gaatgtcttt ccgtcccaga tcgaggaagc actgctggca gtggaaggca ctgcgcccca
     5461 ttaccagatt gttctgacca atgacggcac catggatgat gtggaggttc aggtggaagt
     5521 ttctccggaa gcgttcagcg acaaagtgag cgtcatggaa ggactacgca agaaactgag
     5581 cgcttccatt gagcgcgtag tgaatatccg tgtgaatgtg cggctcgtcg agccgaataa
     5641 aatcgcccgg agcgaaggca aggcaaaacg cctgatcgat aaccgaaaaa aataacggcg
     5701 gcactgcatg cctgtaagga aaggactgtt gcgtgggtga cagcgtccgg ccaggcagcg
     5761 ggcagaaaaa gagataggag acacgtcatg aaaatgcatc aattgtccat gtttctggaa
     5821 aacaaacccg gacgtctgag tgagccctgc cgcctgctgg cgaatgcagg catcaacatc
     5881 ctcaccctct ctgtcgccga cacccagcag tttggtattc tgcgccttat cgtgcccgat
     5941 tgggaagcgg cgaagggcgt gcttgaaaaa gccgggtttg tcgtaaatgt gacggaagtg
     6001 gtggcaacgg aagtggttga tcagcccggc ggtcttgcaa ccattctgga gatcatcgaa
     6061 gaagtggggt tgaacataga atatatgtat gctttcactt tccgctgcaa cgacaaagcg
     6121 gtaatcgtct tccgtttcga caacaatgat atcgccgtga cggcactgca ggcgaaaggg
     6181 atcagtgtgg taggcggtgt ggagctgtac gagcgcgccc gccagtaata ttcctgtttg
     6241 ccggaacatc aaatgatccg catctctcgt ttcagcttcc gatgccggag aactctataa
     6301 aaaggctgct ccatgaacac ctttgccatt gacaaccaga tcctcgataa agaagaaaca
     6361 atgccccgta ctgagctgca ggcactgcag ctgaaacggc tgcgggaaac agtggcacgc
     6421 gccatgaatg tgccttttta taaaaaggaa tttgccgaac ggggcattac ccccgactcc
     6481 attcagacgc tgtcggatgt gcggcgcctt cctttcacaa ccaaagagga tatgcgcagt
     6541 aattaccccc tcggactctg cgccgtacca cgcacggaac tcgcccgcat tcacggttcc
     6601 tccggaacaa caggcatgcc cacctttgtg ggatataccc accaggatct gaagacctgg
     6661 gcacgccttt gtgcccgttt tctggttgca ggcggactac gccccggaca tacagcgcag
     6721 attgccttcg ggtacggcct ttttaccggc ggcttcgggc tgcattacgg cgtggaagaa
     6781 gtgggagcgg ccgtactgcc cgtatcttca ggcaacacga cacggcaggt gcagctgatg
     6841 cgcgatcttc agcctgatgc gctgatctgc acgcccagtt atgcgctgaa tattgcggaa
     6901 gtggcacggg gcatgggcat tgatccccgg acactgagcc tgcgcttcgc tcatttcggc
     6961 ggcgaa
//
LOCUS       DAOPWB010000154         6936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10871_length_6936_cov_3.725912, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000154 DAOPWB010000000
VERSION     DAOPWB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_6936_cov_3.725912"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PKN92_11490"
     CDS             complement(<1..552)
                     /locus_tag="PKN92_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNZ49458.1"
                     /translation="MPKPIYAPDKITALYCRLSRDDGGDAESNSIGNQKTILRRYDTD
                     HGFSNTKYYVDDGWSGANFNRPGFEALMADVDNGLISTIICKDMSRFGRDYLHVGLYT
                     EVKFPEAGIRFIAINDGVDSASGASDDFTPFRNIINEWYCRDISKKIKASMQSRAKSG
                     EHLTGNAPYGYKKDENNPKKWVID"
     gene            complement(626..967)
                     /locus_tag="PKN92_11495"
     CDS             complement(626..967)
                     /locus_tag="PKN92_11495"
                     /inference="COORDINATES: protein motif:HMM:NF025304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNZ49459.1"
                     /translation="MEVLILKRLSDTQKNEIQAMRAEGLGYKAIADKLSLSRETVRSY
                     CKRNRVSAGSTDTAVTDTASEMKQGNTVFIITTAYSENATETLEKKLEKLILNEAAKL
                     SGSYQFVQKSA"
     gene            1032..1172
                     /locus_tag="PKN92_11500"
     CDS             1032..1172
                     /locus_tag="PKN92_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49460.1"
                     /translation="MRIIICTSLCLDFSMIFIVVLSVLLRPAIQAENEVGRTHRKEAP
                     LP"
     gene            complement(1213..2415)
                     /locus_tag="PKN92_11505"
     CDS             complement(1213..2415)
                     /locus_tag="PKN92_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNZ49461.1"
                     /translation="MKNYTINELRETRARAWEQAKAFLDSHHDAKGMVSAENVAKYEA
                     MEEEITNLGKQIEILERREAMDAELAKPVGTLLTNRPGMTGDAKTGRASDAYKNAMLT
                     ALRTNFRTVNNVLMEGNDASGGYLVPTEYDARLIEALEHENVIRSLGTVIQTSGERKI
                     NVAASKPAASWIEENGALVFGDATFDQVILDAYKLSVAVKVSEELLADNAYDLESFLI
                     NAFGRAIANAEEEAFLVGDGNAKPTGLLHATKGGQIGVTTASNTITADEIIDLVYKLK
                     RPYRANAVFITSDSTLAAVRKLKNDNGYLWQPALTAGEPDRLLGFPVYTSQYVPTIAD
                     GQPVMAFGDFSYYNIGDRGARSFAALHELYAGVGQVAFVAKERVDGKLILPEAVQVLK
                     MKGTAAQG"
     gene            complement(2560..3807)
                     /locus_tag="PKN92_11510"
     CDS             complement(2560..3807)
                     /locus_tag="PKN92_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNZ49462.1"
                     /translation="MSIFQNIFKPRGSNHLAGSGPRFFFGQSASGANVNERTAMSMTA
                     VYGCVRVLAESIASLPLHVYKRGENGNREKAESLPLYTLLHDTPNEEMSSFTLRETLM
                     THLLLYGNAYCQILRNGRGEVIAIYPLLPNRMTVERDENGQLFYRYQRYSEEPPTMDG
                     NQVILSPEDVLHIPGMSFDGLVGLSPIAACRNAVGAGLSADEYSSRYYANGAAPMGVL
                     EHPGVIKDPEKLRDSWNAAYGGTKNAGRVAILEEGLKFTPISISPADSQLLETRKFTV
                     EEICRIFRVPPHLVQDLTKTSYNSAEQMSQEFVYYTLLPWCVRIEQAMMRSLLSAEDR
                     KHFAIRFNLDGLLRGSYESRMRGYQTAVNTGIFSVNDCRKLENMDLLTPEDGGDTHMV
                     QGAMIPLSMVGAAYTKQADNKTE"
     gene            complement(4004..5596)
                     /locus_tag="PKN92_11515"
     CDS             complement(4004..5596)
                     /locus_tag="PKN92_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009658143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HNZ49463.1"
                     /translation="MRDYEPTRFMLPTSHYDKGKADRAVMFIESLRHTKGSYYNKPFK
                     LLDWQERLIRDLFGVVKEDGTRQFKQCITFCAKKNGKTELAAAIALFLLCADHEQRAE
                     IYGAAADRQMAGLVFNVAADMIRLSPALRKRCKILDSRKRIIFTPTNSFYQVLSSDAD
                     RAHGVSAHGVIVDEVHVQKNPDLVNVLTKGSGDARKQPLQFIISTAGDNIHSIGYELF
                     QKAKDILDGRKTDPTIYPVVYAADPEDDWTSPEVWRKANPSMGVTFPESAIQEACESA
                     KQNPSEENVFKTLRLNIWTKQAVRWMPMEQWDKCAAPVDAEALRGRPCYAGLDLSSTQ
                     DLTALVLVFPPTNPEEPYSILPFAWVPEETITQRSRRDHVNYDLWRKQGFILATEGSV
                     VDYAAIETKIMELREIYDIREIAYDRWNSQMLIQHLSGEGMTVVPFGQGMQSMSPPTK
                     ELFKLTLEKKLAHGGHPVLRWCMDNVVVDQDAAGNIKITKARATEKVDLAVALVMALD
                     RAVRHEMNKGESVYENRGLLFV"
     gene            complement(5681..6202)
                     /locus_tag="PKN92_11520"
     CDS             complement(5681..6202)
                     /locus_tag="PKN92_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HNZ49464.1"
                     /translation="MAKDGTNRGGKRQGAGRKPKPLAEKLQEGKKAAVLALPENDPYD
                     GPEIESIKEFLSSDQRMGDLYGKEIFEQMVTWLRDRNCAHLVSPHLLEQYSMAMARWI
                     QIENVTSEYGLVSKHPTTGAMIGSPFVTMAQGNLKQANMLYQQIFSIVTANSMEPVTG
                     NPQDDIMEKLLGM"
     gene            complement(6655..>6936)
                     /locus_tag="PKN92_11525"
     CDS             complement(6655..>6936)
                     /locus_tag="PKN92_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49465.1"
                     /translation="VCIDELNWRYSISKASPIFQEQEDRRCGLLIPECVMKYPHADWV
                     YNNGAWECGNCGEEGGKWYDPDWQPTLEQCPYCGAIMGDKDAPFPIVKV"
BASE COUNT         1490 a         1924 c         1737 g         1785 t
ORIGIN      
        1 gtcgattacc cacttcttcg gattattttc gtcctttttg tacccataag gcgcattccc
       61 cgtcaggtgt tcgccggact tggcccgcga ctgcatgcta gccttgattt tctttgaaat
      121 gtcgcggcag taccattcgt tgatgatgtt gcggaaaggg gtaaaatcat cggacgcacc
      181 gctggcgctg tcaacgccgt cgttgatggc gataaagcgg attccagctt ccgggaactt
      241 cacctcagtg tacagaccca catgcaagta atccctgccg aagcgggaca tgtccttgca
      301 gatgatcgta ctgatcagcc cgttgtctac atcggccatc aaggcttcga aaccgggtct
      361 gttgaaattt gcgccgctcc agccgtcatc gacatagtat ttagtgttgc tgaagccgtg
      421 atcggtatcg tatcgcctca ggatggtttt ctggttccct atgctgttcg actcggcgtc
      481 gccaccgtca tcccttgaca agcggcagta gagcgccgtg atcttatcgg gcgcatatat
      541 tggttttggc attttacaac tcctccttga atcgattgtc cagtcgtatt agtcactcag
      601 aaaggcacac attgcaagtc attactcagg cagatttctg aacaaactga taacttccgg
      661 acagctttgc agcctcgttc aggatgagtt tttccagctt cttttccaac gtctcggttg
      721 cgttttcgct gtatgcggtt gtgatgatga aaacggtgtt gccttgcttc atctcagatg
      781 cagtatccgt aacagcggta tctgtgctcc cagcagaaac gcggtttctt ttacaatagc
      841 tcctaaccgt ttcgcgagac aatgagagct tatccgcgat cgccttatat ccaaggcctt
      901 ccgcccgcat agcctgtatt tcattcttct gggtgtctga gagtctcttc aatatcaata
      961 cctccatatc aaaattgcct ggtcatgtat tgacacccag aagatgggcc ctcacatgca
     1021 ggcgggatta catgcgaatc atcatttgca cctcgctttg tctcgatttt tcgatgattt
     1081 tcatcgtcgt tctcagtgtg ttactccgcc cagcgattca ggcggaaaat gaagtgggac
     1141 gcacccacag gaaagaagcc cctcttccgt gagcacgtcc cttggttttt agcaactcat
     1201 atgagcgtta tctcagccct gggctgcagt acccttcatc ttgagcacct gcacagcttc
     1261 gggcaggatc agcttgccat cgacgcgctc cttggcgaca aaggccacct ggccgacacc
     1321 ggcatacagc tcatgcagcg cagcgaaaga acgggcaccg cggtcgccga tgttgtaata
     1381 gctgaaatca ccaaaagcca taacaggctg accatcagca atggtgggaa cgtactggct
     1441 ggtatatacc gggaagccca gcagacgatc cggctcaccg gcagtcaggg cgggctgcca
     1501 cagatagccg ttgtcgttct tcagctttcg tacagcggcc agtgtgctgt cactggtgat
     1561 gaagacggca ttggcacggt agggccgctt cagcttgtac accagatcga tgatttcatc
     1621 agcagtaatc gtattactgg cggtcgtcac accgatctga ccgcctttgg tagcatgaag
     1681 caggcccgtg ggcttcgcat taccgtcacc gacgaggaac gcttcttctt cagcattggc
     1741 gatagcacgg ccaaaagcgt tgatcaggaa ggattccaga tcataagcgt tatccgccag
     1801 aagctcctcg gaaaccttca cagccacgga gagcttgtaa gcatccagaa tcacctggtc
     1861 gaaggtcgcg tcaccgaaaa ccagcgcgcc gttttcctca atccaggagg cggcgggctt
     1921 gctggccgcg acgttgatct tgcgttcgcc ggaggtctgg atcacggtgc ccagggagcg
     1981 gatcacattc tcatgctcca gagcctcgat cagacgggca tcatattccg tcggaacgag
     2041 ataaccgccg gaagcgtcgt tgccctccat cagcacattg ttgacggtgc ggaagttcgt
     2101 gcgcagagca gtcagcatgg cgttcttgta ggcgtcggag gcgcggccag ttttagcgtc
     2161 gccagtcatg ccggggcgat tcgtcagcag cgtgccgaca ggctttgcca gctcggcgtc
     2221 catggcttcg cgccgttcca gaatctcaat ctgcttgccg agattggtga tttcctcttc
     2281 catcgcctca tacttggcga cgttctcagc gcttaccatg cctttggcat catggtggct
     2341 gtcgaggaaa gccttggcct gctcccaggc gcgggcacgg gtttcacgga gttcgttgat
     2401 ggtataattc ttcatgaaga tatcctttct gcccagttat cgtgatgggc acacaaaaaa
     2461 gctccgcata cagatttacg gagcgtaggg attgacggcg ttgagccatc tgtgtatata
     2521 aagaacagca gcgggagggg cttctcctac tgctgcgctt tactctgttt tgttatcagc
     2581 ctgtttcgta tacgctgcac cgaccatgga cagcggaatc attgcgccct gtaccatgtg
     2641 ggtatctccg ccatcctcag gcgtaagcag atccatattc tccagcttac ggcagtcatt
     2701 gacgctgaag atgccggtat tcacggcggt ctggtatccg cgcatacggc tttcatagga
     2761 acctcgcagc aggccatcca gattgaaacg gattgcaaaa tgctttctgt cctcagctga
     2821 cagcagggat cgcatcattg cctgctcaat gcgcacgcac catggaagaa gagtgtagta
     2881 gacaaattcc tgactcatct gttcggcgct attgtaactg gtctttgtca gatcctgaac
     2941 caagtggggc ggcacacgga agatacggca tatttcttca acggtaaatt tccgcgtctc
     3001 cagcagctgt gaatccgccg gggagatgga gatcggcgtg aacttgagcc cctcttcaag
     3061 aatcgcgaca cgcccggcgt ttttggtgcc tccgtatgct gcgttccagg aatctcttag
     3121 tttctctgga tctttgatca cgcctggatg ttccagcacg cccatgggtg ccgctccgtt
     3181 cgcataatac cggctgctgt attcgtcggc ggacaacccc gcgccgacgg cattcctgca
     3241 ggccgcgata gggcttaaac ctaccagacc gtcaaagctc atgccgggga tatggagtac
     3301 atcttctggt gataagatca cctgattgcc atccatcgtc ggaggttcct cgctgtaccg
     3361 ttgataccga tagaacagct ggccgttctc atcccgttcc accgtcatac ggttcggcaa
     3421 caacggatag atggcgatca cctctccgcg cccgttccgc aggatttggc aataggcatt
     3481 gccgtaaaga agcaggtgcg tcataagggt ttcccgcaga gtgaaactcg acatctcttc
     3541 gttgggcgta tcatgcagaa gagtatataa cggcaggctc tctgctttct cccggttccc
     3601 gttttcgccg cgtttataca catgcagcgg cagactggcg atggactccg ccaggacgcg
     3661 tacgcagccg tataccgctg tcatggacat agccgtccgt tcattgacat tcgccccgga
     3721 agcggactga ccgaagaaga atctcgggcc gctgcctgcc agatgattgc tgccacgagg
     3781 cttgaagata ttttgaaaaa tgctcatcca ttatcctttc tgctctgcac atcggagctt
     3841 ctcgttcagc caattttagc aaaacggtta aatgtgattt tccgacagga aaaagagtac
     3901 ttgatttgat gaagtgttgg tccttttacc accagctgga ccttgacgca tgggttataa
     3961 tgagcttgaa aagctcatta ggggaacaga tttacagagg cactcagacg aacagcaggc
     4021 ctctgttttc atatacagat tcgcctttgt tcatctcatg cctgaccgct cgatccagcg
     4081 ccatgaccag cgccaccgca agatcgactt tttctgtcgc ccgcgccttt gtgattttga
     4141 tattaccggc ggcgtcctga tccacgacca cgttatccat gcaccatcgc aggactggat
     4201 gtccgccatg ggccagcttc ttttccagtg tcagtttgaa cagctctttt gtcggtggag
     4261 acattgactg catgccctgg ccgaagggaa cgaccgtcat accttcacca ctgagatgct
     4321 gtatcagcat ctgggaattc cagcggtcat acgcgatctc acgaatgtcg tatatttccc
     4381 gcagctccat gattttcgtc tcgatagctg cataatcgac aacagatcct tcggttgcca
     4441 ggatgaagcc ctgcttacgc cagaggtcat aattcacatg gtcacgcctg gaccgctgtg
     4501 ttatcgtttc ctccggcacc catgcgaagg gcaggatgga gtatggttcc tccggatttg
     4561 tgggcggaaa caccaatacc agcgccgtga ggtcctgtgt actggaaaga tcgagtcctg
     4621 cataacacgg tcggccacga agcgcctctg cgtcaacagg agcagcgcat ttatcccatt
     4681 gctccatagg catccagcga acagcctgct tcgtccagat gttgagccgc agggtcttga
     4741 atacattctc ttcggacggg ttctgtttcg ctgattcaca tgcttcctgg attgcggatt
     4801 ccgggaaggt cacgcccatg gaggggttcg ccttcctcca gacctccggg ctcgtccagt
     4861 catcctccgg gtcggcggca tacacgacag ggtaaatcgt ggggtcggtc ttgcggccgt
     4921 ccagaatgtc ttttgccttc tggaatagtt catatccgat ggagtggatg ttgtcacccg
     4981 ctgtagagat aatgaactga aggggttgct tacgcgcatc accgctgccc ttggtcagca
     5041 cattcaccag atctgggttt ttctgtacat gaacctcatc cacgatcacg ccatgggcgg
     5101 atacgccatg cgcccggtca gcgtcggagg aaagcacctg atagaaactg ttcgtcggcg
     5161 tgaatatgat tcgcttcctg gaatccagta tcttgcacct tttccgcagc gccggagaca
     5221 gccggatcat atccgccgct acattaaata ccagacctgc catctgccgg tcagctgcag
     5281 ccccgtatat ttccgcccgc tgttcatgat cggcgcagag gagaaacagg gcaatcgcgg
     5341 ccgcaagttc cgtcttcccg tttttcttgg cacagaaggt gatgcactgt ttaaactgcc
     5401 tggtgccatc ttcctttacc acaccgaaaa ggtcacggat tagcctctcc tgccaatcga
     5461 gcagtttgaa cggcttgtta taatagctgc ccttggtgtg cctaaggctc tcgatgaaca
     5521 taacggcacg gtcagctttt cccttgtcat aatgactggt cggcagcatg aaccgtgtcg
     5581 gctcataatc cctcatttag attctcctcc tttccgcagt acagggattc acccatgcaa
     5641 atatttgctt gtaagcacct atgaaaatat tttcttacca tcacatcccc agcagttttt
     5701 ccatgatatc gtcctgagga ttgcctgtta ccggctccat actgtttgca gtgactatgg
     5761 agaaaatttg ctgataaagc atatttgcct gtttcagatt gccctgcgcc atggtcacga
     5821 acgggcttcc gatcatagct ccagttgtag ggtgttttga tacaagtccg tattcgctgg
     5881 ttacattttc aatctgtatc caacgcgcca tcgccatgct gtattgttcc agcaggtgag
     5941 gactgaccag atgagcacaa ttacggtcac gcagccaggt aaccatctgc tcaaatatct
     6001 ctttgccgta aagatcgccc atccgctggt ccgaagacaa aaactctttt atgctttcaa
     6061 tctcaggacc atcataggga tcattttctg gcagcgccaa aaccgccgcc ttttttcctt
     6121 cctggagctt ctccgccagg ggcttcggtt tacgtcccgc gccttggcgt ttcccacccc
     6181 ggttggttcc gtcttttgcc atgaatactc ctttctgcca ccgtgggcgg cggctcttca
     6241 agatggtgca gattctgcag cgtcttctta tcaaaataat ttgtggcact tcagaaggtg
     6301 cacatatttg caccttcttc tcgctatgca gtatagattt cagactttcg tctgccaaag
     6361 ttggcacaca aggaattgtt tgattccttt gatttcgttt gaaataacgg cgcagaaaat
     6421 caaaaaggca gaggatcccg gattggagga ttgtcctccc agaatgtacc cgaaatgcct
     6481 tgcctgtaag gattttctga cctgccgctg atttctgaaa accctgctgc cgtgcgggtt
     6541 aatagggtct atgatatccg aggcacgcct gacttgaggg gcgacggtct ccgtccggaa
     6601 gcaggaaatc ttgaggccta cccccggtca aaatcaaacg gaaatcaaac gttttcaaac
     6661 cttcacgata gggaacggcg catctttgtc acccataatc gctccgcagt acgggcactg
     6721 ttccagcgtc ggctgccaat cgggatcata ccatttgccg ccttcttctc cacagttgcc
     6781 gcactcccat gcgccgttgt tatacaccca atcggcatga ggatatttca tcacgcactc
     6841 tgggatcaat aacccgcacc ttctgtcctc ctgctcctgg aagataggcg acgccttgct
     6901 gatgctgtag cgccagttca gctcgtctat gcaaac
//
LOCUS       DAOPWB010000155         6906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_10942_length_6906_cov_4.718143, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000155 DAOPWB010000000
VERSION     DAOPWB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10942_length_6906_cov_4.718143"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKN92_11530"
     CDS             complement(<1..654)
                     /locus_tag="PKN92_11530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNZ49466.1"
                     /translation="MATVKCPACAFQNPNDATNCEKCKQALPRVAFRNSPPPKAAAPQ
                     PSANDALQFSRGQVVNNRYTVLGIIGRGGMGCIYKVHDNVLGEDLALKTLLPQFTSEK
                     MVVDRFLNEARITRKLTHPNIVRVHDIGTAGRGIFISMEYVEGESLRGMLQKLPKGQR
                     LPLQQTLYIIDQLCVALEYAHRYTVHRDIKPENVMITPDNQIKLMDFGISKLMDNRFS
                     "
     gene            complement(814..2292)
                     /locus_tag="PKN92_11535"
     CDS             complement(814..2292)
                     /locus_tag="PKN92_11535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HNZ49467.1"
                     /translation="MKDTHRFLAFDLGAESGRAVLGTLQGGRLSLEVVHRFRTEGLTI
                     LGVRQWDLARIYEEICQGLRQCVRLHGPDLDGIAVDTWGVDFGLVASDGTVLANPRHY
                     RDKANDGMQEYAFAKVPRPELYAATGIQFLPFNSVYQLLGYARRQSPLLDSAESLLLM
                     GDLFGYLLSGVRSCEYTNASTTQMLDPYTRTWNRELLDKLGIPKRLLLNPVMPGTVLG
                     PILPELAEETGVNPDVPVIATASHDTASAVAAVPAASDGEAWAYLSSGTWSLLGAELD
                     GPHVSDASLAEDFTNEGGVDGTIRFLKNIFGLWLVQECRRSWEKADGSSLDYGELTAQ
                     AEAAAPFVSFINLDDSRLMAPDNMPALIQTMCKEAGYPVPESRGALIRCALESLAVKY
                     RTTLRKLDGLLGRETRVLHIIGGGVQNKLLCQMTADACGIPVIAGPVEATALGNIGVQ
                     AMAVGALSGLEDFRAVIRESEQPERYEPVNTEAWDRAIATMK"
     gene            3133..3723
                     /locus_tag="PKN92_11540"
     CDS             3133..3723
                     /locus_tag="PKN92_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49468.1"
                     /translation="MMTEDLGYIDYRTLLDLQDHYKPADVIRTYRKKIKQLMVQISED
                     KTAEDHQDRYLLLMAELNAAYYILRNRALGEQYIQEREEVVALEKEWRALDTADPGFD
                     ALRRRYDQALRSFLARYMEELILEAGRDPECVEHSGWGPAHERLAGRVLRQFRQQRYH
                     EIHERLPYYDITEPQVDWEERSRFAAALISGGQHNG"
     gene            3716..4789
                     /locus_tag="PKN92_11545"
     CDS             3716..4789
                     /locus_tag="PKN92_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49469.1"
                     /translation="MAETVPTEKNIDYALLDRCLAAAIACGDIVNLRFLFLPASPFRR
                     DSSEDISMSKYAYLLAEEESDALEAALRLVQQAEISRQVREQLEKKGPPQLPWELLQA
                     LADNALRLGKYTAASQAYELLRTRRRMQEIFLDQADAALDRGAYAEGARGYKIAAGLQ
                     YDYAAFPEALPAVLNYQEKAVTLHGKYPVVLEGETLSDDRALCRSSLLFLLQGADFIQ
                     RLENRDDESLIQFTAEMIRCLDPAWDRFVPAFQEACRLISPFAELFSRINSYTQEALE
                     VLLEEIFSDEEKETLRGISQFFAPGIAEGSAWQLVMRTLAYYHPGAVSFVRRQRLSAS
                     EEILIPALPDTSRLAQQLGLFPL"
     gene            4890..6107
                     /locus_tag="PKN92_11550"
     CDS             4890..6107
                     /locus_tag="PKN92_11550"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ49470.1"
                     /translation="MNSFTKFRLSVMMFLQYVAPGALIPILTLYLRDHLHFTSYQSGI
                     VMAMPALAAVLAPFAASHIADRMLSAERMLVWCHLLAGGLMLLLARVTRYPAFVGLYF
                     LYGVAFMPTFGLTNAVALHRICDARRDFGGIRLWGTVGWLVIAWLFGYLWVGGGTGQE
                     RMPHALLFSGGASFLLAVFSFIFIPPSEAKRDRVITDYGAVLRIFMKPEMLLLCLLTL
                     LTSACHQFYYYGMSPYLSQIGVADRFIMPTMSLGQASEAIVLGVMGWCLFYLRIKTAM
                     VLGVLAQGMRLLVFAFFPQIASVLGGIGLHGICYAFFFTTGYLYVERHSTRTTRAGAQ
                     QILTIMISGLGVLLGSWSAGWTAQIFSDAGSGHVDFRMFWLVPAVLCIFITLALVAGF
                     HERPASEPEREDV"
     gene            complement(6104..6697)
                     /locus_tag="PKN92_11555"
     CDS             complement(6104..6697)
                     /locus_tag="PKN92_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49471.1"
                     /translation="MTQEKHTPDIWDSLSFIVWCLLFLIGLFPEIVFLQVRAYAHLIS
                     KEAFTNTPLFITFAATGFLSWFTYSRCRLLGDSDDYAILKSTHVALMTFFAFFPVHVE
                     HLPLYLAIPVPFFRYLLTGAVAFKAFLWVVLLLFLLQYHMFRGLAAFRKIPLIFWLKI
                     LASYRDEEKAAAVAAGSSAALEGETEKSSGESPTGEN"
BASE COUNT         1409 a         1830 c         1972 g         1695 t
ORIGIN      
        1 gctgaaacgg ttgtccatca gtttggaaat gccgaagtcc atcagtttga tctgattgtc
       61 cggcgtgatc atgacgtttt cgggtttgat atcccggtgc accgtgtagc gatgggcata
      121 ttcgagggcg acacacagct gatcaataat gtagagcgtc tgctgcaggg gaagtcgctg
      181 tcccttcggc agtttctgga gcatacccct gagtgattcc ccctcgacgt attccatgga
      241 aataaaaata ccgcgtcccg ccgtaccgat gtcatggacc cgcacaatat tgggatgcgt
      301 cagtttacgg gtgatccgcg cctcgttcaa aaagcggtcg accaccattt tttcgctggt
      361 gaattgtggc aacagtgttt tgagtgccag atcctctccc agcacgttgt catgcacttt
      421 gtagatgcag cccatgccgc ccctgccgat aatgccgagc actgtatagc ggttgtttac
      481 cacctgtccg cgcgaaaact gcagggcatc attagcactc ggctgagggg ctgccgcttt
      541 gggcggcggg gaattacgaa aggcgacacg gggcagtgcc tgtttgcatt tctcgcagtt
      601 tgttgcgtca ttgggatttt gaaaagcgca tgcgggacac ttgacggttg ccatactgct
      661 acctcggcgg actattgaaa atgattgcga aagcaccttc attgtaccac aaagcgggcg
      721 ccctgccgcc tcggatagaa ggctcatttt tcagatttgt ccgaaaccgc agcgttcttc
      781 gctgtgcctt ccggaatctg ctttacagca gcgtcatttc atcgtggcga ttgcccgatc
      841 ccaggcctct gtgtttacag gctcatagcg ttccggctgc tctgattccc ggatgaccgc
      901 gcggaagtct tcaagaccgg acaaggcgcc caccgccatt gcctgcacgc cgatattgcc
      961 cagagcggtt gcttccacgg ggccggcaat gaccgggata ccgcaggcat cagccgtcat
     1021 ctggcacagc agtttgttct gtacgccgcc gccgataata tgcagcacac gggtttccct
     1081 gccgaggagt ccgtccagct ttctgagcgt tgtccggtat tttacagcca ggctctccag
     1141 ggcgcagcgg attagggcgc cccgtgattc gggtaccggg tagccggctt ccttgcacat
     1201 ggtctgaatc agggcgggca tattgtcggg tgccatcagt ctgctgtcgt caagattgat
     1261 gaaagagacg aagggcgcgg ctgcttccgc ctgtgctgtc aattcgccgt agtccagcga
     1321 cgagccgtcc gctttttccc aggaacgtct gcattcctgc acgagccaca ggccgaaaat
     1381 atttttgagg aagcggatgg ttccgtccac gccgccctca ttggtgaaat cttccgccag
     1441 gcttgcatca ctgacatggg gaccgtcgag ctccgcaccg agcaacgacc aggtgccgct
     1501 ggaaagatag gcccaggctt caccgtcgga agcagcaggt acggcggcaa cggcagaagc
     1561 cgtatcatga gatgccgtgg cgatcacagg cacatccggg tttacgccgg tttcttcggc
     1621 aagttccgga aggatcggcc ccaggacagt acccggcatc acagggttca gcagcagccg
     1681 tttgggaatg cccagtttat ccagcagctc acggttccag gtgcgggtat agggatcaag
     1741 catctgggtg gtggaagcgt tggtgtactc acagctgcgc acacccgaca gcaggtagcc
     1801 gaacagatcg cccatcagca ggagtgactc ggcggaatcc agcagcggcg actggcggcg
     1861 ggcatagcca agcagctgat atacggaatt aaagggcaga aactgaatgc ctgtggcggc
     1921 atacagctcc gggcgcggca ctttggcaaa ggcatattcc tgcatgccgt cattggcctt
     1981 gtcgcggtag tggcgcggat tggcgagcac ggtgccgtcc gatgccacca gcccgaagtc
     2041 cacgccccag gtgtcgacgg cgataccgtc cagatccggt ccgtgcaggc gtacgcactg
     2101 gcggagcccc tgacatattt cttcgtaaat acgtgccaga tcccactgac gtacaccgag
     2161 tatggtcagc ccttccgtgc ggaagcggtg caccacttcc agcgaaagtc gccctccctg
     2221 aagcgtacct aaaacggcac ggccgctttc agcacccaga tcaaaggcaa gaaaacgatg
     2281 tgtatccttc atgatgtgct cctcttcagc cggcatcccg gggttgtctt ctacagatac
     2341 catacttatt tcacaggcat tcttgcaagt taagacggtg ttttctgtgc ggttcgtgcg
     2401 aaagagcggc ggaccgggtt accatgagaa ggggagcgct ttccttgcat gcgacgtttt
     2461 gttgtcccgc tccttttatt tgtattctgg actacaaatc aatgagtgct gtctaagcgg
     2521 cgccccaggc aggtactgca tgcctgtttc tctgtaaaag acgggataac aggcagggag
     2581 cctgatataa agaggaaggt tatcttcttc aatatcaata tgatagactg atatttcggg
     2641 cgggaaaggg cggtggattt gtgtcgccat aattttccgt ggaagtggaa taacggtccc
     2701 ggaaatatgt tatactttgt atagacagtt gaattactca ggtctttgac caatcatgta
     2761 tccctgttcc gcttccgtac cggtgcggct tttcgggcac atcgggctgc gtagcggtta
     2821 tagtgaaaac tagaaacaga agtccaatat ctttgttaac ctttcgttct tcgcgaacgt
     2881 tttcaaagta agaacgacaa aataaaaccg tacatgagca gacccattcc tcgcaagagc
     2941 gcggaccgtg cctccctcct tccctccccc gccatacggt ccgcgccaat tttttttgcg
     3001 ttctccctcc cataatttct acaatgccat tattcaacag tgcgccgaga gcacctttcc
     3061 ctgaaagagc cgtgtctttt gtggtgacaa gccggttccg gaaaggcgct gttttttttt
     3121 ccgggagaaa aaatgatgac agaagatctg gggtatatcg attaccgtac cctgctggac
     3181 ttgcaggatc attacaagcc tgccgatgta atccgcacct accgtaagaa gatcaagcag
     3241 ctgatggtgc agatcagcga agataaaacc gcagaagatc atcaggaccg gtatctgctg
     3301 ctgatggcgg agctgaatgc ggcgtattac attctccgga accgggcgct gggcgagcag
     3361 tatattcagg agcgggaaga ggttgtggca cttgaaaaag aatggcgtgc tctggatacg
     3421 gcagaccccg gctttgatgc gctgcgccgg cgttatgatc aggcgctgcg ttccttcctt
     3481 gcgcgttaca tggaagagct gattctggaa gcgggccgtg atccggagtg tgtggagcac
     3541 agcgggtggg gtcctgccca tgaacgtctg gcgggcagag tgctgcgcca gttcaggcag
     3601 cagcgttatc acgaaattca cgaacggctg ccgtattatg atattaccga gcctcaggtg
     3661 gactgggaag agcggtcgcg cttcgccgcc gccctgatca gcgggggaca gcacaatggc
     3721 tgaaactgta ccgacagaaa aaaatatcga ttatgccctg ctggatcgct gtcttgccgc
     3781 cgccattgcc tgcggtgata tagtgaacct tcgctttctt tttcttccgg catctccttt
     3841 ccggcgggat tcgtccgagg atatttccat gtccaaatat gcatacctgc tggcggaaga
     3901 ggaaagcgac gctctggaag ctgcgctgcg tctggtgcag caggcggaga tctcccggca
     3961 ggttcgggag cagctggaga aaaaagggcc gcctcagctt ccctgggaac ttctgcaggc
     4021 cctcgccgat aatgctttac gtctgggcaa atatacggct gcgtcacagg cttatgaact
     4081 gcttcgcacc cggcggcgca tgcaggaaat atttctcgat caggcggatg ccgctctgga
     4141 tcgcggcgcg tatgctgaag gggcacgggg gtataaaatt gctgcagggc tgcagtatga
     4201 ctatgccgcc tttccggaag cgctgcctgc cgtgctgaat tatcaggaga aggcggttac
     4261 ccttcacgga aaataccctg tggttcttga gggggagaca ctgtccgatg acagagcatt
     4321 atgcagaagc agtcttctgt ttctgctgca gggtgccgat tttattcagc gtcttgaaaa
     4381 tcgggacgac gaaagcctca tacaattcac ggccgagatg atccgctgtc ttgatccggc
     4441 gtgggaccgc tttgttcctg catttcagga agcgtgccgg ctgatcagcc cctttgcgga
     4501 gcttttctcc cgcatcaaca gttatacgca ggaagcgctg gaggtgctgc tggaagagat
     4561 tttcagcgat gaggagaaag aaacgctacg gggcatttcc cagttttttg cgccgggcat
     4621 tgccgaggga agcgcgtggc agctggtcat gcgcaccctg gcttattatc atcccggtgc
     4681 tgtgtctttt gtccggcgcc agcgtttatc ggcgtcggaa gaaattctga ttcctgcgct
     4741 tccggatacg tcccgtctgg cacagcagct ggggctgttc cctttgtgaa accggcgagt
     4801 cgtgtgtctc tcttcttctt tgcccgaaaa ggaaaccggt attttcgcgg cattttcacg
     4861 acagccatgc tgacggaagg aattattctt tgaattcatt tacaaaattc cgcctgtccg
     4921 tcatgatgtt tcttcaatat gttgcgccgg gcgccctgat tcccatcctt acgctgtatc
     4981 ttcgggacca tttgcacttt acgtcctacc agtccggcat tgtgatggca atgcccgcat
     5041 tggcagctgt cctggctcct tttgcggcgt cccatatcgc cgaccgtatg ttgtcagcag
     5101 agcgcatgct ggtctggtgc cacctgcttg caggcgggct gatgctgctg cttgcgcggg
     5161 tgacgcgcta tcccgctttc gtagggcttt attttcttta tggcgtagcg ttcatgccta
     5221 ccttcggtct gaccaatgcc gtggcgcttc acaggatttg cgatgcgcgc cgggactttg
     5281 gcggtatccg gctctggggc acagtcggct ggcttgtcat tgcctggctc ttcggctatc
     5341 tgtgggtcgg cggcggtacg ggccaggagc gcatgcccca tgcccttctt ttttccggtg
     5401 gcgcatcttt tcttcttgca gttttttctt ttatttttat tccgccctcc gaggcgaagc
     5461 gggatcgtgt gattaccgat tacggtgctg tgctccgtat tttcatgaag ccggaaatgc
     5521 tgctcctctg cctgctcacg cttctgacca gtgcctgcca tcagttttac tattacggca
     5581 tgagccctta tctgagtcag atcggtgttg cggatcgctt cattatgcct actatgagtc
     5641 tggggcaggc ctctgaggcg atcgtgctgg gcgtgatggg ctggtgcctg ttctacctgc
     5701 gcattaaaac agccatggtt ctgggcgtgc tggcgcaggg tatgcgcctt ctggtctttg
     5761 cttttttccc gcagatcgct tcagtcctgg gaggcatcgg gctgcacggt atctgctatg
     5821 ccttcttttt cacaacgggc tacctttatg tagagcgaca cagcacccgt acgacccggg
     5881 caggggcgca gcagattctg actattatga tctcgggtct gggtgtgctg ctcggttcct
     5941 ggtcggcggg gtggacggcg cagatttttt cggatgcggg cagcgggcat gttgattttc
     6001 gtatgttctg gctcgtaccc gccgtgctgt gtatctttat tacactggca ctggtggcgg
     6061 gctttcatga acgtcccgct tctgagcctg agcgggaaga cgtctagttt tcgccagtcg
     6121 ggctttcccc ggacgacttt tctgtttccc cttccagtgc agcggaactg cctgctgcga
     6181 ccgccgccgc cttttcctcg tccctgtagc tggccagtat tttaagccag aaaatcaacg
     6241 ggattttccg gaaggcggcg agtccgcgaa acatatgata ctgcagcagg aaaagaagca
     6301 gtacgaccca caggaaggct ttgaaagcaa cggcaccggt cagtaaatat ctgaaaaagg
     6361 gcacaggaat agcaagatac agggggagat gttccacatg gacagggaaa aaagcgaaga
     6421 aagtcatcag agccacatgg gttgatttca gaatagcgta gtcgtcgctg tcgcccagaa
     6481 gcctgcagcg gctgtaggtg aaccagctca aaaaacctgt tgcggcgaaa gtgatgaaca
     6541 ggggtgtgtt ggtgaaggct tctttcgaga ttaaatgggc atacgcccgg acttgaagga
     6601 agacgatttc cggaaacagc ccgatcagaa aaaggagaca ccagacgata aaagaaaggc
     6661 tgtcccagat atccggcgta tgtttttctt gtgtcacagg tagtcccggt ttgaagttcg
     6721 taaagtgact tttctgcccg cacttccgca ttaaggcaga ggctgcctgg ctattccttc
     6781 cacaatcgga aaagacgcat tattgtactt gcccggggcg ggtgtatgcc acttttctct
     6841 tttgtgtgtg cggggagatg ctgaaacacg tctattcatt ttggcagcca cccctgcggc
     6901 agtggc
//
LOCUS       DAOPWB010000156         6834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_11098_length_6834_cov_5.841570, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000156 DAOPWB010000000
VERSION     DAOPWB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11098_length_6834_cov_5.841570"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /gene="glgP"
                     /locus_tag="PKN92_11560"
     CDS             complement(<1..1866)
                     /gene="glgP"
                     /locus_tag="PKN92_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNZ49472.1"
                     /translation="MPKIIKYIVAPRLPETLQRLSDIADNLWWAWNLDAVDLFFRMDR
                     DLWTQVEHNPKRLLREISQERLEQLAESASFLAHLDRVATKLDAYMTEGEWRSRHSDA
                     PEDLLVAYLSAEFGFHESITIYSGGLGVLAGDHLKAASDLGLPLVGLGLLYREGYFRQ
                     YLNADGWQQELYPKNDFYAMPIQAVRDAAGSHQVVEVPYPGRMVKAYLWKCQVGRVPL
                     YLLDTDHEDNSPTDRSITGQLYGGDHDTRIKQEIMLGMGGVIALKALGLHPTTYHINE
                     GHAAFMMLERIKDLIERDQITFNEAVEVVKVGSVFTTHTPVPAGNDVFDPAMINAYFR
                     DYCAAAGISMDTLLALGRENPADSKEKFCMTILALKLSAASNGVSALHGEVARKMWAP
                     VWKGVPEEELPITSITNGVHIPTWISRDLALLFDRYLGPDWLAMPHDQSVWERVDSIP
                     DSELFQTHVRACQRLVDFARSRAEKQMLNRGASAAEVRTAGELLNGETLTIGFARRFA
                     TYKRATLLFKNPERLKKILTNPERPVQLIIAGKAHPQDSSSKELIRHIIHFSRDPQVR
                     SHIVFIEDYDMNVARYMLQGVDCWLNTPLRPNEASGTSGMKAAANGALNISIPDGW"
     gene            complement(2429..2605)
                     /locus_tag="PKN92_11565"
     CDS             complement(2429..2605)
                     /locus_tag="PKN92_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49473.1"
                     /translation="MRHIYTLSSKPEQAAGLAPDVVITLIIDLLKVFLPLFQNKNPQN
                     PGTGTDPKPPEDDK"
     gene            complement(2999..3154)
                     /locus_tag="PKN92_11570"
     CDS             complement(2999..3154)
                     /locus_tag="PKN92_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49474.1"
                     /translation="MKHIIRLSNPVERAATTSTGAYIVLAGQILAILAGLFDGKEVLK
                     PDAQAED"
     gene            complement(3274..4227)
                     /locus_tag="PKN92_11575"
     CDS             complement(3274..4227)
                     /locus_tag="PKN92_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HNZ49475.1"
                     /translation="MKRKGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQNN
                     LKQWGIIYKMYSSESPGNKYPKVCLATVQTRFCEQPGMPFSGANSVVAAGMYLPSLYP
                     EYLTDAKILVCPSDAVETADSVINPVTGTTDIGVACQEKKRGVHLADASYLYLGWVLD
                     KAGSTDPSVDLASVPIPGLNVSGPAPKQVVDGVLAAFMPFLLSQDDRMADKDVEVDPG
                     LGNGAGNMIYRLREGIERFLITDINNPAASAMAQSTLWIMGDMIGIDPNLYNHVPGGS
                     NVLYMDGHVQFQRYEEKGSAPVNSGVGRCVAALQAVQNLLL"
     gene            complement(4441..5775)
                     /gene="tolB"
                     /locus_tag="PKN92_11580"
     CDS             complement(4441..5775)
                     /gene="tolB"
                     /locus_tag="PKN92_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNZ49476.1"
                     /translation="MKKLFIFKKRSVWFLSAVLFLSVNLISQAQSGVRIESGSTVDRR
                     IRIAVPPFATVDPQLQGMAAEMAQALADDLEFSGVFILLPAASYPAGFTGMSTDPSTL
                     DLPAWRATQAENLVYGLVFYEGSQLVAQFRLFDLYSNTQLYGQEVKVAREHHRLASHR
                     FSEEVIRQIDGTPGIGTSEIIFSGLAGANQKEIYVADYDGANVKRVTNHQSLSIMPEI
                     SPDSRSIAYLSYKDRYAFHYIFDRATGASRALSKEVGLNSAATWSPDGKRLALTLSKD
                     GNAEIYLKNPDGSGSLRLTNNRENDTSPCFSPDGSQICFVSERAGRPQIYVMNADGSN
                     PHRLSFHGGSAYDPNWSPDGKSIAFVSNISGEGFEIYVMGADGSNPTRLTNSNGINEA
                     PSWSPDSRHIAFSSTRNGRPELFTYTLSTRTERAIARLGMSAEGPSWGPRRR"
     gene            complement(5892..>6834)
                     /locus_tag="PKN92_11585"
     CDS             complement(5892..>6834)
                     /locus_tag="PKN92_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49477.1"
                     /translation="EVPGEGESEGEISEGEVPSEGESEGEISEGEVPGEGESEGEISE
                     GEMTEGESAPEEKLALSLKRWADGDQRYLPDTLLSLNISLTAFGEGIPESLTLEEQLP
                     RNWTFTELHDPSGLISSHKNSEGTITFVWSALPTFPVQFSYDVLPGASNETETIDGSA
                     FYTVNGTTLRSNQEQSQFRRYETLLSLERKEAPSGYIPGKPLAVTLQWKRIGLEKPLA
                     FACVELLPAGWRFAGIVEATGNPPDILPEMDQENMLAFGWTTTPTAPGSFTYKVLPPE
                     DARGIACITGYSLYRFTGEEQKTALTGRCYPRGSFQP"
BASE COUNT         1561 a         1761 c         1865 g         1647 t
ORIGIN      
        1 ccatccgtcg ggaatactga tgttcaaagc gccgtttgct gccgctttca tgccactggt
       61 tccgctcgct tcgttggggc gcaggggcgt attgagccag cagtcaacgc cctgcagcat
      121 gtagcgcgct acattcatgt cgtaatcctc aataaatacg atgtgagagc gcacctgagg
      181 atcgcgggaa aaatgaatga tatgcctgat gagttctttg gagctggaat cctggggatg
      241 tgcttttccg gcgataatca gctgaaccgg cctctcggga ttggtgagaa tctttttcag
      301 ccgctcgggg tttttaaaca gcaacgtcgc acgcttatag gtggcaaagc gccgggcgaa
      361 gccgatggtc agcgtttctc cgttcagcag ttcacctgct gtccggactt cagcggcaga
      421 agcgcctctg ttgagcatct gtttttcggc acggcttcgg gcaaaatcaa ccagccgctg
      481 gcatgcgcgt acatgggttt gaaaaagttc ggaatcaggg atggaatcca cccgttccca
      541 tacagactga tcatgcggca tggcgagcca gtccgggcca agatagcggt caaagagcag
      601 cgccagatcg cgggagatcc aggtggggat atggacgccg tttgttatgg aggtaatggg
      661 cagttcctct tcgggaacgc ctttccatac aggtgcccac attttgcgtg caacttcccc
      721 gtgcagtgca ctgacgccat tggaggcggc ggaaagcttc agagccagaa tggtcatgca
      781 gaacttttcc ttgctgtccg cagggttctc acggcccagc gccaggagtg tatccatgga
      841 aatgcctgca gcggcacagt aatcccggaa ataggcattg atcatggcag ggtcgaaaac
      901 gtcgtttcct gccggtacag gggtatgggt tgtgaagacg ctgcccacct tcacaacttc
      961 caccgcctca ttgaaggtga tctgatcccg ttcgatgagg tctttgatcc gctccagcat
     1021 cataaatgcg gcatggccct cgttgatatg gtaggtggtg ggatgcagtc ccagtgcttt
     1081 cagggcgatg acgccgccca ttcccagcat aatttcctgt ttgatgcggg tgtcgtgatc
     1141 gcccccgtat aattgaccgg tgatgcttct gtctgtgggc gagttatcct cgtgatccgt
     1201 atcaaggaga taaaggggaa ctctgcctac ctggcatttc cagaggtagg ctttgaccat
     1261 tcttcccgga taggggactt cgactacctg atggcttccg gcggcatcgc gcacggcctg
     1321 aatgggcatt gcgtaaaaat cgtttttggg atacagttcc tgctgccagc cgtcggcgtt
     1381 gagatactgc cggaagtagc cctcccggta taaaagcccc agacccacca gaggaagccc
     1441 cagatcgctt gccgctttca ggtgatctcc ggcaaggacg cccagaccgc ctgagtaaat
     1501 ggtgatggat tcatgaaaac cgaattcagc cgataagtag gcaacaagca ggtcttcagg
     1561 cgcatcgctg tggcgggagc gccactcgcc ctcagtcata taggcgtcca gcttggtggc
     1621 gactctatcc agatgggcaa ggaagctggc gctttccgcc agttgctcca accgttcctg
     1681 actgatttcg cgcagaagtc ttttgggatt gtgttccacc tgtgtccaga ggtcgcggtc
     1741 catgcggaaa aacagatcta ccgcatcgag attccacgcc caccaaagat tgtccgcaat
     1801 atcggaaagg cgctgcagtg tctccggcag ccggggagca acaatgtatt tgataatttt
     1861 aggcattggt taattcctgt cgggttatat cctgtggggg tgggaagaaa atccggggcc
     1921 ggaagataaa gaaaaagccg acgctttctc tgcaacatgg cgttgccggt gctttatttc
     1981 cagccgctgc gggggcgtgg gcggaaagcc tcacatgcgg gtattatagc atgtcagaca
     2041 caaaaagccg accttttagc gttaaaggtc ggcttggtat tttctattgg gctggatcag
     2101 ccgtaatatt cgtttttctt ctggaagcgg aaatcataaa aagcgcggta gactgtgtac
     2161 agaaagtcct ggaacgccat ggcttttctg gcagaaagtt tcttaatgtg tttcattatt
     2221 cgttcctcct gacgctgttg cttatacata tttggattgg cgaaaatcgt accaggcgcg
     2281 ccagagcgta ttaaacaggg tcagaaaagc atcggcacgt ctgacagtca tcttccggat
     2341 gtgtttcatc gtaataactc cattgcgatg cattgcacat gcacgctact tatagcatat
     2401 tgcgcctgca aacacaaaat aattccgttt acttgtcatc ttcgggaggc ttcggatctg
     2461 tgcccgtgcc gggattttgc ggatttttat tctggaaaag cggaaggaac actttcagaa
     2521 gatcaatgat cagcgtgatg acaacgtccg gtgccaggcc tgctgcctgt tccggtttgc
     2581 tgctgagcgt gtaaatgtgt ctcatggctg gttcctcgtt tgctccgggc ggatagtgtt
     2641 tactcttcag tggcaggagg tgtggtggtg ggcgcagcct tgatcgctat caggggctgt
     2701 accagtggca cgaaaacctg cgagaaaaag gaaaggacaa aacagaaccg ggcctgcaga
     2761 tcactggtga tatcagtgca aagaccatcg gtttgtgctg gccggggcaa gcgccccatt
     2821 cttcttatgt gcttcatttt acttctccaa tttcagcata tcactgtgtc aatgctgtta
     2881 tcatcgcaca gcgcctatgg aatttacaat ttacgggttt ataaagatgc atgacccact
     2941 gataaactaa ttcccgtatc agccggaaag ttcccgtcgg ctatttttat ttcggttttt
     3001 aatcttcagc ttgtgcatcc ggctttaaga cttccttgcc gtcaaagaga cccgccagga
     3061 tggcaagaat ctgacctgcc agaacaatat aggcgcctgt agatgtagtg gctgcccgct
     3121 cgacaggatt gctcaatcgg ataatatgtt tcataaccgg tctctccatt ttccgacggc
     3181 cagcacatcc cggcggaatc gtctgtttta attctatcta ttctaccacc ggaatttaaa
     3241 agggttacag atgatataaa gaaggcagaa cggttacaga agcagatttt gtaccgcttg
     3301 cagagccgca acacatcgtc ccacgccgct gttcaccgga gccgaccctt tttcttcata
     3361 acgctggaac tggacatgtc cgtccatgta caggacattg gagccgccgg gtacatggtt
     3421 ataaagattg gggtcaatgc cgatcatgtc gcccatgatc caaagggtgc tttgcgccat
     3481 ggcgcttgcg gccggattgt tgatgtcggt aatgagaaag cgctcaatgc cctctctcag
     3541 acggtagatc atattgccgg cgccattgcc cagcccggga tccacttcca catccttgtc
     3601 cgccatccgg tcgtcctgac tcaggaggaa gggcatgaat gccgccagta caccatcgac
     3661 cacctgcttc ggtgcggggc cgcttacatt caggccgggg ataggcacag aggcaagatc
     3721 gacgctggga tcggtggatc ctgccttgtc cagcacccag cccagataaa gataactggc
     3781 gtctgccaga tggacacccc gttttttctc ctgacatgcc acaccgatat cggtcgtgcc
     3841 tgtaacgggg ttgatgacag agtctgcagt ttcgacagcg tcagaagggc agaccagaat
     3901 cttcgcatcg gtcaggtatt cagggtagag agacggcaga tacataccgg cggcgacaac
     3961 gctgttcgca ccggagaagg gcatacccgg ctgttcacaa aatcgtgtct gcacggttgc
     4021 caggcagacc ttcgggtatt tatttccagg tgactcgctg gaatacattt tgtaaatgat
     4081 gccccattgt ttcagattgt tctggcagct ggacctccgc gcagcctcgc gggcacgggc
     4141 gagtgcgggc aggagaatcg ctgccaggat gccaataatg gcaattacaa caagaagctc
     4201 aatcagggta aagccttttc ttttcataat gaagacactc cgaaaaagat gatgggtata
     4261 aatgcgggca ctgctcttca aggagacaaa aaagcccccg gcagtgcgaa atattaaaaa
     4321 agtatacaga acatccggaa aggatatcca cttttaaacc aaactgtaca gaaagtacag
     4381 cgcaataagg cctttcagcc gaagagatat gtagtgccta tgttggtacc ctggctgcaa
     4441 tcagcggcgg cgcggtcccc aggagggtcc ttcagcgctc atgcccagcc gggcgatcgc
     4501 ccgttctgtg cgggtgctca gggtgtaggt gaaaagttca gggcgcccgt ttcgcgtgga
     4561 agagaaagca atatgtcttg agtcgggcga ccaactgggc gcctcgttga tgccgttgga
     4621 attggtgagc cgcgtcgggt tggaaccatc ggcgcccatc acatagattt caaacccttc
     4681 tccggaaata ttggacacaa aagcaataga cttgccatcg ggtgaccagt tcgggtcata
     4741 agcgcttccg ccatggaagg atagcctgtg cggattggat ccgtccgcat tcattacata
     4801 aatctgaggg cggccggcgc gctcgcttac aaagcagatc tgacttccgt cgggcgagaa
     4861 acagggcgaa gtatcatttt ccctattgtt tgtaaggcga agggagccgc tgccgtccgg
     4921 gtttttaagg taaatttcgg cattcccgtc tttgctcagt gtcagtgcca gccgtttgcc
     4981 gtcgggggac caggttgcgg cactgttaag ccccacttct ttggacaggg cgcgggatgc
     5041 tccggtggca cggtcgaaaa tataatggaa cgcataccgg tctttgtacg acagataggc
     5101 aatggagcgg ctgtccggcg aaatttcggg cataatggac aaactctggt gattggtaac
     5161 ccgcttgacg ttggcgccgt cgtagtccgc cacataaatc tctttctgat tggcgcctgc
     5221 cagtccggaa aaaatgattt cggatgtgcc gataccgggc gtaccgtcaa tctgtctgat
     5281 tacttcttcg gaaaagcgat gagaagcgag cctgtgatgc tcccgggcga cttttacttc
     5341 ctgtccgtag agctgggtat tggagtaaag gtcaaaaagg cggaactgcg ccaccagttg
     5401 tgagccttcg taaaatacaa gcccgtaaac cagattttcc gcctgtgtcg cacgccaggc
     5461 aggcagatcc agggtggagg gatcggtgct catcccggtg aatcctgccg ggtaactcgc
     5521 tgccggaagg agtataaaaa cgccggaaaa ttcaaggtca tcggcaaggg cctgcgccat
     5581 ttccgccgcc atgccctgaa gttgcgggtc aacggtggca aaaggaggca cggcaatgcg
     5641 gatgcgccgg tccacggtgc tgccggactc gatccgcacc ccgctttgtg cctggctgat
     5701 cagattgacg gacagaaaca aaactgcaga taaaaaccag acacttcttt ttttaaagat
     5761 aaaaagtttt ttcattacga gtaacccttg catcgtaatt tagaatcagg cgacagactg
     5821 catgtgcagc tgcctcctcc caatatccac actgttcagt tgccggctgt ccatacacag
     5881 cctctgtatc atcacggttg aaaagatccc cggggatagc agcgcccggt caatgctgtt
     5941 ttctgctcct cgccggtaaa acggtaaaga ctatatccgg taatacaggc aatgcctctt
     6001 gcatcttcag gcggcagcac cttataggta aaagatccgg gtgcggtcgg tgtcgttgtc
     6061 cagccgaagg cgagcatatt ttcctggtcc atttcgggaa gaatgtccgg cggattaccg
     6121 gtagcttcaa caatgcctgc aaaacgccat cctgcaggta ataattccac acaggcaaaa
     6181 gccagcggct tttcaagacc gatgcgcttc cactgcagcg tgactgccag aggcttcccg
     6241 gggatatatc ccgaaggtgc ctcttttcgt tccagagaga gcagtgtttc gtaacgcctg
     6301 aactggctct gttcctgatt cgaacggagc gtggtaccat tgaccgtata aaaggcactg
     6361 ccgtcaatgg tttcggtttc attggatgca ccgggaagca catcgtagga aaactgtaca
     6421 ggaaacgtgg gcaaagcaga ccagacgaag gtgattgtgc cctcactgtt cttgtggctg
     6481 ctgatcaggc ctgaagggtc atgtaattct gtaaaagtcc agttgcgggg caactgttct
     6541 tccaaggtga gtgattcggg gatgccctca ccgaaagcag tcaaagaaat attcagggaa
     6601 agcagggtat cgggaagata gcgctgatct ccatccgccc atcttttcaa ggagagagcg
     6661 agtttttctt caggcgcact ttctccttcc gtcatttcgc cttcgctgat ttcgccttcg
     6721 ctctcaccct caccgggcac ttcgccttcg ctgatttcgc cttcgctctc accctcactg
     6781 ggcacttcgc cttcgctgat ttcgccttcg ctctcaccct caccgggcac ttcg
//
LOCUS       DAOPWB010000157         6816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_11125_length_6816_cov_4.645467, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000157 DAOPWB010000000
VERSION     DAOPWB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11125_length_6816_cov_4.645467"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PKN92_11590"
     CDS             complement(<1..745)
                     /locus_tag="PKN92_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNZ49478.1"
                     /translation="MHTRRLLTPGFLVLFLVSSTAAQHAALHSAVMPTPRDKSSWLER
                     HEAINARIEQGQVNLLWIGDSIIEGWEDSGKSVWDHYYAHRQAVNLGISGDRTEHVLW
                     RLDHGNIKDIAPALAIVMIGQNNGPHNSAEEIAEGITAIVERLRSNLPDAKILLLGVF
                     YRGEKPDNEWEKLSAVNRTIAALADNAHVFYRDVNYCFHREDGTINPRLMPDYEHPGE
                     EGYALWAQAMEGIISRLLGDNAIDGASPYL"
     gene            complement(772..3210)
                     /locus_tag="PKN92_11595"
     CDS             complement(772..3210)
                     /locus_tag="PKN92_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNZ49479.1"
                     /translation="MKLFSVLSFLLVFAFFAATAEADTVSCLRFPDDTTLALASEMLG
                     VELLPEKGSDSQPMVILPEAAAQYLREQGLSFDILWTRTEITVGMPADKAEAVAYGNG
                     SGFAINFRNQQSVTIGHAPANAKVSHVSITLSGVAAYADLCTFSIISSTGKKYTLPKW
                     YDEIWFDHTVSGIQAFKGEKVNQRWTLEGQGSNTAGERVNSWTIGIFYTESSGGNGNE
                     NPEGEIIPNPVPVVYWYWWVKCRAPYYAFVEKSVLGQRYLLVDFKLPVMPTNDSCAFH
                     ELGRPVFSIDAKTRQIGVQFIPEPNRPCLTVMDPVCAGRTLIGPLSAGTWQFSSRAKN
                     LPPLTESFTVTKVQSHEEKMALVPDVAGKTAAQAKTLIQEAGLKTGVHRYEFSDSVAK
                     DLVIGCGHFYGELVPAGSTVSLIISKGQNTDITDPEGETVREGENGNLGLTDVPNCIL
                     LLLAEAKQYIRSAGLQIGTVTEEHHKNIPAGYVSHQYPAANSVVFPGTSVNLTVSLGR
                     TPQIAVPDVTGTDLMDADQRLQHAGLELGRVDFSHDETAPYNQVLEQIPAAGMLVAPG
                     SAVKLVLSKGPLPENGEAIELPNLLLQTLSEAQALLEKTGFSTGSIEKAHDENVPYGR
                     VISQSPSAYRYLPPGTPIDLVISLGAEPRIGVPSLAGMDALEALDMLEELALVPGIIE
                     QVFDTLIPAGHVSGQDPAPGTRVKKGSSVNLRISNGRDPDELTENEGEGNTDVDPPAR
                     EGEPDEALPDEGETTAPDGNEEALPEEGEIPEREKEPVSACDGCSGSGKALGDYLLLG
                     CVMLLLAQSSFFRK"
     gene            complement(3310..4929)
                     /locus_tag="PKN92_11600"
     CDS             complement(3310..4929)
                     /locus_tag="PKN92_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNZ49480.1"
                     /translation="MQTSSTVPLRLRFFSAVLTGLMLCLFSPAHARPASGFVSVKDGR
                     FIDPEGRHLLLHGINIVSKNRQGNYQPRVTEKDFEQLRAWGWNCIRFGVSWDGLEPEI
                     NRFDEAYLEKLDGWIALAKQYGFYVFLDMHQDLFNHKYGNGAPDWATLDDGMPHLRGA
                     IWSDAYMISPAVQTVFDNFWANKPCADGVGVQDHFAAAWKKLAERYKDEPAVIAYDLF
                     NEPNIGSGNAAIILEVLNAYVSNTDKLAEGDKPLTLDEVMQPNPQRNLRSVLAKQMQN
                     LELYQALLDAAAPLCVDFESTRLTAMFQRVRDAIRSVDQNHIIFLETNISANMGVPTG
                     VQPLVDETEQRDPLLAYAPHAYDIVVDTHDLELAFNGRLELIFRRHAESGKRLDMPVL
                     IGEWGAFGRGSAGILPSARFTVAMMEKYLMSNTYWDYSYGLNDSPCHEIFERPIARNT
                     SGILLSCATDFEKGSFECRWKEDPTIKAPTCIFLPERMAPYLDAICVAPAGTGWSVTR
                     PYENSAAAVIEIPAAGGQEERTFFIAPPETD"
     gene            complement(5032..5325)
                     /locus_tag="PKN92_11605"
     CDS             complement(5032..5325)
                     /locus_tag="PKN92_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNZ49481.1"
                     /translation="MNVYVGNLSYSTSSEDLRELFSAYGEVTSANVISDRDSGRSKGF
                     GFVEMANAEEARTAISKLNGAEVSGRNITVNEARPREERAPRRGGFGGGRNRY"
     gene            complement(5432..5785)
                     /gene="ssrA"
                     /locus_tag="PKN92_11610"
     tmRNA           complement(5432..5785)
                     /gene="ssrA"
                     /locus_tag="PKN92_11610"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5888..>6816)
                     /locus_tag="PKN92_11615"
     CDS             complement(5888..>6816)
                     /locus_tag="PKN92_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49482.1"
                     /translation="RPYKLDTSAVPYEVPVRVDYTDERGRTQAQLDFFVAMGPVFRID
                     FETLNSSRIILQASEDGISFTPVPLKALLPESASRGPLFIDPPISRRYLRLQVESTGE
                     QAVLREVRAFAWKKKPVALCPAVTVGADGLRSWPEEGRITGLVAQETGRFAKAPTEFR
                     LAHNGTHLFIGVIAQDPTPHAATAVMTGNDDPLWEEESVEIRIKCGNSAARRFIINPK
                     GARYDAMDSLGDSNQWDPGWDADWRAQAEQTAGGWSATVALPFTILGGTPGRTENWQI
                     NFHRHRKNIEEENSLWALPAYPESPAYGELCF"
BASE COUNT         1516 a         1860 c         1821 g         1619 t
ORIGIN      
        1 gaagataagg agatgccccg tcaatggcat tatctcctaa cagccgggag ataatacctt
       61 ccattgcctg tgcccagaga gcatagcctt cttcgccggg atgctcataa tcaggcatca
      121 gcctgggatt gatcgtgccg tcttccctat gaaaacagta attcacatcc ctgtaaaaga
      181 cgtgcgcatt gtcggcaagc gccgcaatcg tcctgttgac agcgctgagt ttctcccatt
      241 cattatccgg tttttccccc cgataaaaga cgccgagcag cagaattttt gcatccggca
      301 gatttgaacg gaggcgctcc acaatggcgg tgatgccttc cgcgatttct tcagcgctgt
      361 tatgcggccc gttgttctgc ccgatcatca caatggcgag agcgggcgca atatccttga
      421 tatttccatg atccagccgc cagaggacat gttctgttct gtctccgctg attcccagat
      481 taacggcctg gcgatgggca tagtagtgat cccagacaga cttaccgctg tcttcccacc
      541 cttcaataat ggaatcgcca atccacagaa gatttacctg accctgttca atgcgggcgt
      601 tgattgcctc gtgccgttcc agccaggaag atttgtcccg gggtgtcggc atgacggcac
      661 tgtgaagagc agcatgctgg gctgctgttg atgaaaccag aaacagtacc aaaaaaccgg
      721 gtgtcaacag gcgccgggta tgcacgaaag agcgtccttt cagaagtaag tctattttcg
      781 gaaaaaagaa ctttgcgcaa gaagcagcat aacacatcca agaagcaaat agtcccccaa
      841 ggcttttccg ctgcctgaac agccgtcgca ggcggaaacg ggctcttttt ccctttccgg
      901 tatttcacct tcttccggca atgcctcttc gtttccgtct ggtgctgttg tttctccttc
      961 atcaggaagc gcttcgtcag gctctccttc ccgggcgggc ggatcgacat ctgtattccc
     1021 ttctccttcg ttttccgtca gctcatcggg gtcacgtccg ttggagatgc gcaggtttac
     1081 agaagagccc tttttaaccc gggttccggg tgcggggtcc tgtccgctta catgcccggc
     1141 agggatcagt gtatcaaaga cctgttcaat gatgccgggc accagtgcca gctcctccag
     1201 catatccagc gcttccagcg catccatacc tgccagtgaa ggtacgccga ttcggggctc
     1261 agcaccaagg ctgatgacaa gatcaatggg agtgcccggc ggcagataac ggtaggcgga
     1321 gggactctgg ctgattactc tcccgtacgg cacgttttca tcatgtgctt tttcaatgct
     1381 gcctgtggaa aaacccgtct tttccagaag tgcctgtgcc tcactcagtg tctgcagaag
     1441 cagattgggc agctcgatcg cttcaccgtt ttccggcagg ggacctttgg aaagcaccag
     1501 tttcaccgca ctgcccggag ccaccagcat tcccgctgcc ggtatctgtt cgagcacctg
     1561 attgtaaggg gctgtttcat catgagagaa atcaacgcga cccaattcca gccccgcatg
     1621 ctgaagccgc tggtcagcat ccatcagatc ggtgcctgta acatcaggta cggcgatttg
     1681 cggcgtgcgt cccagactta ccgtcagatt tacggatgta ccgggaaaca ccacactgtt
     1741 ggcggcggga tactgatgtg acacatatcc ggccgggata ttcttatgat gctcttccgt
     1801 cactgtcccg atttgaagcc cggcagagcg aatgtactgt tttgcctcag caagcagaag
     1861 cagaatgcaa ttggggacat cagtcaagcc cagattcccg ttttcacctt cccgtactgt
     1921 ttcaccttcg gggtcggtaa tatccgtatt ctgcccttta gagatgataa gggataccgt
     1981 tgagcctgcc ggcaccagtt cgccgtagaa gtggccgcag ccgatgacaa gatctttggc
     2041 gactgaatcg gaaaattcat atcgatgcac tcccgttttc agcccggctt cctgaatcaa
     2101 tgtctttgcc tgtgctgctg ttttgcccgc tacgtccgga accagtgcca ttttctcttc
     2161 gtggctctgc actttcgtta ctgtgaatga ttccgttaac ggcggcaggt ttttcgctct
     2221 cgaagaaaac tgccaggtgc cggcggacaa tggaccgatc agcgttcgtc ccgcgcaaac
     2281 cggatccatg acagtcagac aggggcggtt tggctcgggt ataaactgga cacctatctg
     2341 acgggttttg gcatcgatgg aaaagacagg ccgacccaac tcatgaaaag cacaggagtc
     2401 gtttgtcggc atcacgggca gcttaaagtc gaccagcaga taacgctggc cgagcacgga
     2461 tttctcaaca aaggcgtagt agggcgcacg acatttcacc caccagtacc agtacaccac
     2521 cggcacaggg ttggggataa tttccccttc ggggttttcg tttccgtttc cgccggagga
     2581 ttcggtgtag aaaatgccga tagtccagga attgacccgt tcacctgcag tattggaacc
     2641 ctgcccctcc agtgtccacc gttgatttac tttttcaccc ttaaaagcct gaatgccgct
     2701 aaccgtatga tcgaaccata tctcatcata ccatttgggc agcgtgtatt ttttccctgt
     2761 gctgctgatg attgaaaagg tacacaagtc agcataggca gcgactccgg agagggtaat
     2821 ggagacatgt gatacttttg cattggcggg ggcatgcccg atggtaacac tttgctggtt
     2881 tcggaaattg atggcaaagc cggaaccgtt gccatatgca acagcctccg ctttatcagc
     2941 aggcatcccg acggtgatct ctgtccgtgt ccacagaata tcaaaagaaa gcccctgctc
     3001 ccggaggtat tgtgcggcgg cttcgggcag aatgaccatc ggctggctgt cgcttccttt
     3061 ttcaggaagc agttcgacgc cgagcatttc ggaggcaaga gccagcgtag tatcgtccgg
     3121 gaagcgcagg caggataccg tgtcggcttc tgccgtagcg gcgaaaaagg cgaagacaag
     3181 caaaaacgac agtacagaaa aaagtttcat ggataatccc ctgttatttc caaaatcctc
     3241 aaaattaccg gtaacggctt attataacca atgattccag aaatgtaaag ggcgcggcgc
     3301 ctccggtatt tagtctgttt caggaggggc aataaaaaaa gtgcgttcct cctgcccacc
     3361 tgccgccgga atttctatga ccgctgcagc gctgttttcg taggggcggg taacgctcca
     3421 tcctgtgccg gcaggcgcca cacagatggc atccagatag ggtgccatgc gctcaggaag
     3481 gaagatgcag gtaggcgctt tgattgtcgg gtcctctttc catcggcact cgaagctccc
     3541 tttttcaaaa tctgtggcgc atgaaaggag gatgccgctt gtgtttctgg cgatgggacg
     3601 ttcaaaaatt tcatggcagg ggctgtcgtt aagcccgtag ctgtagtccc agtaggtatt
     3661 gctcatgaga tatttttcca tcatggcaac cgtgaaccgt gctgagggaa gaatcccggc
     3721 ggaacctctg ccgaaggcgc cccactcacc gatgagcacc ggcatgtcca gacgctttcc
     3781 cgactcggcg tggcgccgga agatcagttc aagccgtccg ttgaaagcga gttcaagatc
     3841 gtgggtgtcc acaactatgt cgtaagcgtg aggcgcatag gcaagcagag gatcccgttg
     3901 ttctgtttcg tccaccagcg gctgcacacc ggtgggcaca cccatgttgg cggagatgtt
     3961 cgtctccagg aagataatat ggttctgatc tacagaccgg atggcatcac gaacccgttg
     4021 gaacatggca gtaagcctgg tgctttcgaa gtcgacgcaa agaggggctg ccgcatcgag
     4081 cagcgcctga tacagttcca gattctgcat ctgtttcgca aggacactgc gcaggttccg
     4141 ctgtggattg ggctgcatga cttcgtccag cgtcagcggt ttatcgcctt ccgccagttt
     4201 gtcggtattg ctcacatagg cgttgagcac ttcaagaata atcgctgcat tgccgctgcc
     4261 gatattgggc tcattgaaaa gatcataggc aatgacggcg ggttcatctt tatatcgttc
     4321 cgccagtttt ttccaggcgg cggcaaaatg atcctgcacg ccgacaccat cagcgcaggg
     4381 tttgtttgcc cagaaattat caaagaccgt ttgcactgcc gggctgatca tgtaggcgtc
     4441 gctccagatg gcaccccgca gatgcggcat gccgtcgtcg agggtagccc agtccggtgc
     4501 accgttcccg tacttgtgat tgaaaagatc ctgatgcatg tcgaggaaga cataaaaacc
     4561 gtattgcttt gcgagagcaa tccatccgtc gagtttttcc agataagcct catcaaaacg
     4621 attgatttcc ggctccaaac cgtcccagct gacaccgaaa cgtatgcagt tccagcccca
     4681 ggcgcggagc tgctcaaagt ccttttccgt tacacgtggc tgataattcc cctgacggtt
     4741 tttgctcaca atattaatcc cgtgcaggag caggtgcctg ccttcgggat cgatgaagcg
     4801 gccgtctttt acggacacaa agcccgacgc agggcgggca tgggctgggg agaaaaggca
     4861 gagcatcaag cctgtgagca cagcggagaa gaacctgagg cgtaaaggaa cagttgaaga
     4921 cgtttgcatg gtattcctta tcctgcacaa gaatgaattg aaagaaaggg aacagaaaaa
     4981 ggccggaacc gctgggattc cggcctctga aggcataaca gggattggta attagtagcg
     5041 gttgcgtccg ccaccgaagc caccccgacg cggggcacgc tcttcacggg gacgtgcttc
     5101 attgactgta atgttccggc cggagacttc cgcgccgttc agcttgctga tggcagtcct
     5161 tgcctcttcg gcattagcca tttcaacgaa gccaaagccc ttagagcggc cggagtcgcg
     5221 atcggagatc acgtttgcgg aagtcacttc accgtaagca gaaaagagct cgcgcaagtc
     5281 ttcgctggaa gtgctgtaag agaggttgcc gacatagacg ttcatactcg agatatccta
     5341 tttgccatga gttcagttgg aatcgaaagt atgcccatgg caacagacca cttcgatgca
     5401 cgtctccctc gagacgatca aaaacagctt atggtggagg cggcgggaat cgaacccgcg
     5461 tccgatagcg cttccatgtc agtatctacg tgcgtagtct ttcttttggg gattcgccct
     5521 tccggaacgc gtcagactcg cttcccggcg tcgctagaca actgtatact ctccccggtg
     5581 cgctgtccgt tcccggattg aggcccgctg tcggcgttcc tgtctgctag caggcagtcg
     5641 caggaggaac gtgctgtctt ttaattaggc agcaagggcg aagttattgt tcgcagttaa
     5701 cttgtaccgg ttttttacga ggccaccgga gtcctcggca cgctgctcac acttcaatca
     5761 ccaccgtcga aaccatgtcg cccccgcatt tatttcatac agacaacttc ctgcaaaagg
     5821 ctccatgcaa attatgccac taatgcttta taaaaagcaa acttttttcc ttttttcaaa
     5881 attcgggtca aaagcacaat tcgccataag cgggggattc aggataagca ggcaatgccc
     5941 agaggctgtt ttcctcttca atatttttcc tgtgcctgtg aaaattgatc tgccagtttt
     6001 cagtgcggcc cggcgtgccg ccgagaattg taaagggcaa tgcaacagtg gcgctccagc
     6061 ctcccgctgt ctgctccgcc tgagcacgcc agtccgcatc ccagccgggg tcccactgat
     6121 tgctgtcgcc gaggctgtcc atggcatcgt agcgcgctcc tttagggttg atgatgaatc
     6181 ggcgtgcggc actgttcccg cacttaatcc ggatttcaac actttcctct tcccacaggg
     6241 gatcatcatt tccggtcatg acagcggtag cggcatgggg tgtgggatcc tgcgcaatta
     6301 cgcctataaa caagtgcgtg ccattgtgcg ccagtctgaa ttcagtgggt gctttggcaa
     6361 agcggccggt ttcctgagcg acgagtccgg taatcctgcc ttcttccggc cagctgcgca
     6421 gcccgtcggc accgactgtt acagcgggac acagagcaac cggtttcttc ttccaggcaa
     6481 aagcgcggac ttcccgaagc accgcctgct cgcccgtact ttcgacctgc agacgcaggt
     6541 aacgcctgct gatgggcgga tcgatgaaaa gcggacctct tgatgcactt tcgggcagca
     6601 gggctttcaa aggtacagga gtgaagctga ttccatcctc gcttgcctgc agaatgatgc
     6661 ggctgctgtt cagggtttca aaatcgatgc ggaaaacggg tcccatggca acgaagaaat
     6721 ccagttgtgc ctgggtgcgt ccccgctcat cggtgtaatc gacccgcaca ggcacttcat
     6781 aaggcacagc gcttgtgtcg agtttgtagg ggcgat
//
LOCUS       DAOPWB010000158         6784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_11194_length_6784_cov_4.623421, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000158 DAOPWB010000000
VERSION     DAOPWB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11194_length_6784_cov_4.623421"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..2624)
                     /locus_tag="PKN92_11620"
     CDS             complement(327..2624)
                     /locus_tag="PKN92_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ49483.1"
                     /translation="MNKYLPILFPLLIIFAGLALRIPYLEEIRHTPEFKYPLYDPEYN
                     AYWAKGLATDDWTVPSGVRDPEIRTTPHGRPPGYPWFLFLIYRLFGVSDTAPRIVQML
                     LGLVNALLLYSLGKRIFGAGTGFLAGLMMALYWVFPYYEGLLTYPALAIFLLLCLFHC
                     LRSWLRQERFWSLALSGILLGAFALLRPNGLLLLPLLLLWILLISLRKKVPLRRLCFS
                     LVLPPVCCIAAVAPAFIRDYVVARDLVFISSYGGINLYVGNHPNASLVEPRIPELMTW
                     AGVDNWSCFDYPAIVRGLAAQQGLESMRFSEANRYFYRAALHFITERPGLFFANLIRK
                     MLLFWGPVEITNDTVPAWDKYFSRILHPLPGFPWFFALFLLGTVCLLVDKEKPRKQTA
                     NDLLLFLWLTIPVYCLSVVLYFVAGRYRVPVIPMVLLFGAYGIGALARSYRAKHYKKA
                     LALTVLLILFFMLSHWHMPGYEASEATWHLRHALACSRSGMPETAEASYLKALEKGAD
                     SAVVYANLGRIHVQRGDREGGMALYRRGLEEHPDNAILHNNLGYELYREGKREEAQQH
                     YLRAIEKNPRLALVRINLGHLLAEEGNREEALAQYEEALRLEPQDCAALYNCARLYFE
                     SGRTTEAIRLYRRVVSLRPDWAEALNNLGYCYATVGQITEALHCYERAVSADPSYTLA
                     WNNLGNARLAMGDEEGAHSAYAAALKERNDDIYALHNLARLFEKQGHQEEAASYCEKV
                     LAIAPDYVPALELLKKLKQTAEQPE"
     gene            complement(2694..3158)
                     /gene="queF"
                     /locus_tag="PKN92_11625"
     CDS             complement(2694..3158)
                     /gene="queF"
                     /locus_tag="PKN92_11625"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNZ49484.1"
                     /translation="MSKKTKKCEFKALDKSIPFLGPEAIDSACLECFPYEYRNTPSGS
                     AMEIRIETDEFTSVCPFSGLPDFATVLIEYVPDELCLELRSLKYYLLSYRNVGIWYEH
                     LVNRMLEDLVAACAPRSMTVEIECLPRGGLSSTVTATYYAEQSKSRSKKSKK"
     gene            complement(3268..3354)
                     /locus_tag="PKN92_11630"
     tRNA            complement(3268..3354)
                     /locus_tag="PKN92_11630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3318..3320),aa:Leu,seq:cag)
     gene            complement(3413..4588)
                     /locus_tag="PKN92_11635"
     CDS             complement(3413..4588)
                     /locus_tag="PKN92_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNZ49485.1"
                     /translation="MSRSVYGICLCMFFLVVRSVPAVDYDIQLDTITSGYDGITCWVH
                     PKAGLIPGDTPVVVMTMQKLLLSGSDVFYALNEMRTDDLGKSWQGPVEHETLGRRMEK
                     GYVSVICDFTPQWHAASKTLLGTGHVARYEGDRLAKDYSRETAYSRYDPEKRSWAAWK
                     SLKMPDDPRFYSSGAGCSQRVDLENGDILQPVYFAPEGEKCYSATVLRCRFDGETLSY
                     VEHGDVLELKEPRGFCEPSLTAFQGRFFLTLRNDIRGYVTRGKDGLHFEEPRPWTFDN
                     GSELGSYNTQQHWVTHQDGLFLVYTRRDPDYDHVFRHRAPLRIAQVDPDRLCVLRHTE
                     RILVPSRGARLGNFSVTPVSDRDVWVTVAEWMQPAGCERYGSDNAVYAARIRWHEPD"
     gene            complement(4585..5694)
                     /locus_tag="PKN92_11640"
     CDS             complement(4585..5694)
                     /locus_tag="PKN92_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49486.1"
                     /translation="MTDRTKNILKNLLALFVGLALTGSMLVAAEGYLWLRARKAAHAA
                     HARLDDNQSEFFIADPILGGKCKEGFKGPHHLRINDKEIFSTYYEFDDHSCRITPVDH
                     ADERTEGALFFGCSFCFGFGVAQDETLPACLAKECDALLPVNFANPGHGPQHMWLLIQ
                     QPDFLKQVPREKGVVLFGFIDHHIPRLMGDKDLVSTWGRRFPWLEITEEGVISRGFME
                     DREANNSSVLKVLQRFHLGRFLVDRLKLIPAKFYTEEEGYHTLARLFTDVRKRLKAQL
                     PDYELIVFAFPGALQGPQLGEVLKEYGIPFLDYSGLYRHFGEEVGDYFFADSLGTRWG
                     HPKAPLYADVAKRLAEDLQEHCHRQGGSEQETEAE"
     gene            complement(5732..>6784)
                     /locus_tag="PKN92_11645"
     CDS             complement(5732..>6784)
                     /locus_tag="PKN92_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49487.1"
                     /translation="NLLALVIGLGLLCTLLCGAELFLRFRAHEDPKDNHKDFFSYVPE
                     LGWQIKENYSGHHRLRVGTEEIFSVHYEFDAHHRRITPVTDADMRQKAALFFGCSFVF
                     GFGVEQEDTLPAFFSRDCTSLLPINFGSPGYGPQHMWLQIQHPDFFASVPREKGIILY
                     GFIDDHLSRLMGEPNMVANWGPRLPWLDISEEGVIYKGLMKDRESVFTALRQKLMRLH
                     TGRFILNRLSLLPVAPYTEEEALQALVWLLSDVKRKVNELLPGYDLVVFAYPGALRAA
                     GLGDALEKTGIPFFNYSRLYDGQEAALGQYYYADAPPNPWGHPKPATYEKVAALLAKD
                     LAEYCGGDENTIGDTL"
BASE COUNT         1692 a         1884 c         1756 g         1452 t
ORIGIN      
        1 aaattaagga ttattttgtg tttgtacgtc cttaagcctt tggtgtggat cagggtgatg
       61 cgcagcgcaa gacgtccata ccctgattaa gattcctgcc ttcgcaggaa ggacggggag
      121 gcaggaagga cggggagtgc aggaaggacg gggagtgcgg gaatgacggg gagtgcagga
      181 atgacgggga gtgcaggaat gacggagagt gcaggaatga cgaggtgtgc gggaatgacg
      241 gggagaggca ggaaggacgg agggaggcag gaatgacggg gagtgcaggg acggcgccga
      301 agcgcctctg gcccggcggg agttttttac tcgggttgtt cggctgtttg tttcagtttt
      361 ttcagcaact ccagcgcggg aacatagtcc ggcgctattg caagcacttt ttcgcaatag
      421 gatgcagcct cttcctgatg cccctgcttt tcaaagagcc gggcaagatt atgcagcgca
      481 tagatatcat cgttccgctc tttgagggct gctgcataag cggagtgcgc tccttcctca
      541 tcgcccattg ccagccgtgc attgcccaga ttattccacg ccagcgtata ggacggatct
      601 gccgacaccg ctctttcata acagtgcagc gcctctgtta tctgaccgac cgtcgcataa
      661 caatatccaa gattgttcag ggcttccgcc caatccggac gaagggacac cacacgcctg
      721 tagagacgga ttgcctcagt cgttcgccct gattcaaagt acagccgggc acagttgtag
      781 agagcggcac aatcctgcgg ctccagccga agcgcctctt catactgagc caaagcctct
      841 tccctgttcc cttcttccgc cagcagatgt cccagattga tccgcacgag agccagccgc
      901 ggattttttt ctatggcgcg gagataatgc tgctgcgctt cttccctttt tccttcccgg
      961 tacagttcat accccagatt attatgcaga atcgcattat ccgggtgttc ttccagcccc
     1021 cgccggtaga gcgccattcc gccttcgcga tcgccccgct gcacatgaat ccgtccgagg
     1081 ttggcataga ccacagcgga atccgctccc ttttccagcg ccttgagata acttgcttcc
     1141 gccgtttcag gcatgccgga gcggctgcag gccagagcat gacgcagatg ccaggtcgct
     1201 tcagaggctt catagccggg catatgccaa tgagacagca taaaaaagag gatcagcagt
     1261 acggtgagtg ccaaggcttt tttatagtgc tttgccctgt agctccgggc aagcgcgcca
     1321 ataccgtacg caccgaagag gagcaccatc ggtatcaccg gaaccctgta ccgcccggca
     1381 acaaaataaa gaaccacgga caggcagtac accggtattg tcagccagag aaaaagcagc
     1441 agatcattcg ctgtctgctt ccttggcttc tctttatcaa caagaaggca gaccgttccc
     1501 agcagaaaaa gagcaaagaa ccaggggaag cccgggagcg gatgcagtat ccgggagaaa
     1561 tatttgtccc aggcgggcac cgtgtcgttt gtgatttcca caggccccca gaagagaagc
     1621 attttcctga tcagattggc gaagaaaagc cccggtctct ctgtaataaa atgcagcgcc
     1681 gcccggtaaa aatagcggtt cgcctcggaa aagcgcatgg attccagccc ctgctgcgct
     1741 gcaagaccgc ggacaatggc gggataatcg aagcaggacc agttatccac ccccgcccag
     1801 gtcatcagtt cgggaatgcg tggctccacg agcgaagcat tcggatgatt gcccacatag
     1861 agattgatgc cgccataaga tgaaataaac accagatccc gggcgacaac atagtcgcgg
     1921 atgaaagcgg gcgccaccgc agcaatgcag cagacaggcg gcagtaccag agaaaagcac
     1981 agacggcgca gcggcacttt ctttcggagg ctgatcagaa gaatccacag aagcagcaaa
     2041 ggaaggagca gcagtccatt gggacggagc agcgcaaagg cacccaacaa tatacctgac
     2101 agggcaagcg accagaatcg ttcctgccgc agccagctcc ggaggcagtg gaacagacaa
     2161 agcagcagaa aaatggcaag cgccgggtat gtcagcaatc cttcataata agggaagacc
     2221 cagtaaagcg ccatcatcaa tccggcaaga aagcctgtgc cggcgccgaa gatccgcttc
     2281 cccaggctgt agagcagcag tgcatttaca agccccagta gcatctgtac aatgcgcggg
     2341 gcagtatcgc ttacgccgaa gaggcggtaa atgaggaaaa gaaaccaggg atagccgggc
     2401 ggcctgccgt gcggcgtcgt cctgatttcc gggtcgcgca cgccggacgg cacagtccag
     2461 tcgtctgtag caaggccttt cgcccaatag gcattgtatt caggatcgta gagcgggtat
     2521 ttgaactccg gtgtgtgtct gatttcttcc aaatacggga tgcgcagggc aagcccggcg
     2581 aagataatca ggagcggaaa aagaataggg agatacttat tcacgacagc ctgccttttc
     2641 gcaaagctgc aggagcggct gcgctgtcat ccggggggct ggcaggcact gcctcatttc
     2701 ttcgattttt tactgcggct ttttgactgt tcagcataat aggtcgccgt gacggtgctg
     2761 gataggccgc cccggggcag gcattctatt tccaccgtca tggagcgggg cgcacatgcc
     2821 gccaccagat cttcaagcat gcggtttacc agatgttcat accatatccc cacgttccgg
     2881 taggacagca gataatattt gagagagcgc agttcgaggc aaagttcgtc cggcacatat
     2941 tcgatgagca ctgttgcgaa atcgggaagc ccggaaaaag ggcatacgga ggtgaattcg
     3001 tcggtttcaa tccggatttc catggcactg cccgacggtg tgttccggta ttcataagga
     3061 aaacattcca gacaggcact gtcgatcgcc tcgggcccca aaaagggaat gcttttatcc
     3121 agcgctttaa actcacattt ctttgtcttt ttgctcatat cctggctcca ccgttgctat
     3181 ctgcccttct gctcagactg cggaaggggc tgtaaacgca aaacgccgtc tctgcactgc
     3241 aggacggcgt taaactcggt aaagaaatgg tgccggagaa aggacttgaa ccttcacgcc
     3301 cttgcgagca catggacctg aaccatgcgt gtctgccaat tccaccactc cggcacggca
     3361 ggtaatactt tacctgattt gatgaaagga agtcaatttt tttccggttc ggtcaatccg
     3421 gctcgtgcca gcggatacgg gcggcataaa cagcgttgtc ggaaccgtaa cgctcacagc
     3481 ctgccggctg catccattcc gccacggtta cccacacgtc ccgatcactc accggtgtta
     3541 cagaaaagtt gcccagccgg gcaccccggg acggcaccag aatgcgttcc gtatgcctga
     3601 gcacacaaag acggtcggga tccacctgtg cgatgcgcag cggcgcacgg tgccggaata
     3661 catgatcata gtccggatca cgccgggtat atacaagaaa aagtccgtcc tgatgcgtca
     3721 cccagtgctg ctgggtgtta tagctgccca gttctgatcc gttatcaaaa gtccaggggc
     3781 gcggttcttc aaaatgcaat ccatccttcc cccgggtcac ataccccctg atatcattgc
     3841 gcaaggtcag gaaaaaccga ccttgaaaag cagtcaggga aggctcacaa aagccgcgtg
     3901 gctccttcag ttccaaaaca tcaccatgct ccacatatga cagtgtttcg ccatcgaaac
     3961 ggcagcgcag cactgtggcg gaatagcatt tctctccttc aggcgcaaaa tagacaggct
     4021 gcagaatgtc tccgttctcc agatcgacgc gctgagagca accggcaccg gaactgtaaa
     4081 acctgggatc atctggcatt ttcagacttt tccaggcagc ccagcttcgt ttctcaggat
     4141 cgtaccggga ataagccgtc tcacgggaat aatcctttgc cagtctgtcc ccttcatagc
     4201 gcgccacgtg ccccgtaccc agcagtgttt ttgaggcggc atgccattgg ggcgtgaaat
     4261 cgcaaatcac ggacacatat cctttttcca tgcgacgtcc cagcgtttca tgctccaccg
     4321 gcccctgcca gctctttccg agatcatcgg tacgcatctc gttcaacgca taaaatacgt
     4381 ccgaaccgct gagtagaagt ttctgcatcg tcatcaccac aaccggagta tcgccgggaa
     4441 tcagccccgc tttaggatga acccaacagg taattccgtc atagccgctt gtgatcgtgt
     4501 ccagctggat atcgtagtcc actgccggca cggatcgaac aacaaggaag aacatgcata
     4561 aacaaatgcc atatacactt ctgctcattc tgcctccgtc tcctgttctg atccgccctg
     4621 cctgtgacag tgttcctgca gatcttcggc gagtcttttt gccacatctg catacagcgg
     4681 agccttcgga tgcccccatc gggttcccag agaatcagca aaaaagtaat cacctacttc
     4741 ttcgccgaaa tggcggtaca atccactgta atccagaaaa ggaatcccgt attctttcag
     4801 cacctcgccg agctgcggtc cctgcaatgc gcccggaaaa gcaaatacaa tcagttcata
     4861 gtccggcagc tgtgctttca ggcgcttacg cacatccgta aaaagccggg caagcgtatg
     4921 atagccttct tcttccgtgt agaacttggc aggtatcagc ttcagccggt cgacgagaaa
     4981 acgccccagg tgaaagcgct gcagcacttt gagaacagag gaattgttcg cttcacgatc
     5041 ctccataaac ccccgggata tcacaccttc ttccgtaatc tccagccacg ggaaacgccg
     5101 accccaggtg gagaccagat ctttgtcgcc catcagtctt gggatatgat gatcaataaa
     5161 cccgaaaaga acaacacctt tttcccgggg cacctgtttg agaaaatccg gctgctgaat
     5221 cagcagccac atatgctgag gtccgtgtcc cggattcgca aagtttacag gaagcagcgc
     5281 atcgcattct tttgccagac aggcggggag cgtttcatcc tgcgccaccc cgaagccgaa
     5341 acaaaaagaa cagccgaaga aaagcgctcc ttctgtccgt tcatccgcat gatccacagg
     5401 cgtaatacgg cagctgtgat catcaaattc gtagtaggtt gaaaatattt ccttatcgtt
     5461 tatgcgcaga tggtgaggcc ctttaaagcc ttctttgcat ttaccgccca gaatcggatc
     5521 tgctataaaa aactcactct ggttgtcgtc cagacgtgca tgtgccgcat gcgccgcctt
     5581 gcgtgcacga agccacagat atccttccgc tgcaaccagc atgcttccgg tcagagcaag
     5641 accgacaaaa agagcaagca gattcttcag tatgtttttt gtgcgatcag tcacggcaat
     5701 tcctttcccg aacggcgaaa caccacacct ttcaaagagt gtcgcctatt gtattctcat
     5761 cgccgccgca gtactcagcc agatcttttg ccagcaaagc ggcaactttt tcataggtag
     5821 ccggtttcgg atgtccccag ggattcggcg gagcatccgc ataataatat tgccccaggg
     5881 ctgcttcctg cccatcatag agcctgctgt aattaaaaaa aggaatccct gttttttcaa
     5941 gtgcatcgcc gagaccggcc gcccgcaaag cacccggata ggcaaaaacg acaagatcat
     6001 agcctggaag caattcattc acttttcttt ttacgtctga cagaagccac acaagcgctt
     6061 gaagcgcttc ttcttccgta tacggcgcaa caggaaggag gcttagacga tttagaataa
     6121 aacgcccggt atgaaggcgc attagtttct gacgcagcgc ggtaaagaca gactcgcggt
     6181 ctttcatcag acccttataa atgacccctt cctcagaaat atccagccag ggaagacggg
     6241 gaccccagtt cgcaaccata ttgggttcgc ccataagcct gcttagatgg tcgtcgataa
     6301 aaccgtaaag gataatgcct ttctcccggg gaacactcgc aaaaaaatca ggatgctgaa
     6361 tctgcagcca catatgctgc ggtccgtacc cggggctgcc aaagtttatc ggcaagagtg
     6421 aagtacagtc acgactgaaa aatgccggca gtgtatcctc ctgttccacg ccaaagccga
     6481 aaacaaagga gcatccgaaa aaaagcgctg ctttctgcct catgtccgca tcggttacgg
     6541 gggtaatccg gcggtgatga gcatcgaatt cataatggac ggagaatatt tcttcggtac
     6601 cgacgcgcag tcgatggtgc ccggaataat tttctttgat ttgccagccc agctccggca
     6661 cataagaaaa aaaatcctta tggttatctt tcggatcttc atgtgcgcga aagcgcagaa
     6721 aaagttcggc gccacataga agcgtgcata agagccccag ccctatcacc agagcaagca
     6781 gatt
//
LOCUS       DAOPWB010000159         6757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_11251_length_6757_cov_4.660101, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000159 DAOPWB010000000
VERSION     DAOPWB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11251_length_6757_cov_4.660101"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKN92_11650"
     CDS             <1..222
                     /locus_tag="PKN92_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49488.1"
                     /translation="QNVHNLIKIPAFAGMTGGEAGMTGGEAGMTGSAGTVESVVTAVR
                     TGVAPETCNSSLGTPISALAGTFQVCDVV"
     gene            complement(300..1676)
                     /locus_tag="PKN92_11655"
     CDS             complement(300..1676)
                     /locus_tag="PKN92_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNZ49489.1"
                     /translation="MAVRILQEKRPPGSTLAWLMAIVLAPYIGVPLYLVIGGRKFNRS
                     IHMIERLYVPGTGQKQEMLVPAAGRIVNQAGFPSPRPGNLVVFHSSGETAYAAMMQLF
                     EAARVSIHVSTFILGDDDTGRAVVKALAKKAREGVEVRLLLDSLGCLTTSRGFVQPIR
                     DAGGRAKRFLPVLPLQRKWSANLRNHRKIVVVDHVAAVVGGMNLAMLFMGAEPDCYRY
                     VDDSVFLYGPAVYDIEEVFRHDWEFAADEQLHLPEGCLFPRTPEPFLNAMIEGVTPFH
                     AVDDCMIQVVPSGPDVPEDTFHDALLTAIMEAEKRIWLMTPYFVPDASLLKPLALKAR
                     SGCDIRIIVPLRSNHGIADLARGPALRELSAAGAAIYVCPAPMIHGKVMLFDHKIAIT
                     GSPNLDMRSIYLNFEIALFHYSPADIDILEAWLRQIQLTCHMIRPREVSRRRAWAENL
                     SHLISPLL"
     gene            complement(1753..3495)
                     /locus_tag="PKN92_11660"
     CDS             complement(1753..3495)
                     /locus_tag="PKN92_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNZ49490.1"
                     /translation="MKRFSLIFAACMTLCAAARGQMIAGTIGPEVSFDIASRPAVIAP
                     GSVFTGAVKVMLNDGWHVNAHEPLDPFLIATELQFEKQTGFTLRGVAYPEAKTVNLDF
                     SPEPLAVYEEVFFLAFEMETDATLAAGEYTLNLSLLFQACNNAMCAAPRSESIPFSVK
                     IGESAGAAEEALPDWFDRAPLSAMQAVTDVNAVPPAETPEESAAIPEQEPPALSADWH
                     ALAEKFRVVNTLAGYTDKEGFLSFIAGDRSDAEGSNAGGLGGLWLLWIVIGGGLLLNF
                     TPCVLPLIPINIAIIGAGTRAGSRSRGFALGGAYGLGIALVYGSLGLLVVLGLSSAFG
                     TINSTVWFNAAIAVLFVVLGLAMFDVIQIDFSRFQTRLGMTPKEGGHFFAALGMGAVS
                     ALLAGACVAPVVIYTILQAQDLYSQGNVFALALPFGLGVGMALPWPFLGAGLSLLPKP
                     GMWMVRVKQAFGVFIIAFALYYGYLAYSLMTPPAIKSDENGIWVHQLEEGLAQALEKN
                     QPVLLDFWATWCKNCTVMSHTVLSDPDVLTALDGYVKIKYQAESLQSVPTRDVVEYYK
                     VLGLPTFVLLEPKE"
     gene            complement(3627..6413)
                     /locus_tag="PKN92_11665"
     CDS             complement(3627..6413)
                     /locus_tag="PKN92_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNZ49491.1"
                     /translation="MAQERKTIHVVSNSHWDREWGYPFEETRLLLLKFMDELLSLLDT
                     DPDFTSFTMDSQTLCVEDYLELRPEKRATIEKHVKSGRLLIGPWYSLPEEYVCNGESL
                     VRNLVVGHRSAEALGGVTKSGYTPFSYGQTSQIPQIYRGFGIDDIIFYRGINTPKCEY
                     ILEGPDGSRLLGSRFGCMSRFSYYIYIYRVLRYGSDDVYQRYDWDRGAAPFRMIASER
                     LRDHYYILDDGRKQWNDAPIREQLLKLVRDESEHFTTSQIVCMQGFDTSNPDPEESRI
                     MKLCQELLPEHDIRFSTLKEYMTAMAKEVKDPIVLRGEFRDPGATGSWTHLFGDIISA
                     RPRLKQNNHKAEVNLQRRAEPWSAIASMLEGEYYKTALARCWKLLLKNHPHDTITGAG
                     VDRMEEDSIYRFNQINLISEGVARRGLQHVQIQVDNSDLSPRDSVITVFNPCPFPRRA
                     VISCLVDMPQNMGYTAFGVQTPEGKSLRVQREEQYPMGVLVRNLQDISSELRTERVRC
                     HLETEDIPAFGYSTYHVTRRPGFEYLPGSLVPETNVLENEHLRVVFNSNGTLDMTHKE
                     TGRVYSGLHYLEDGGETGHSWVHMAPERDEIITSHGAPCYITLEESGPLYARMRVDYH
                     MQIPVSVEDEPYVDFRQGDLNNTGRTKERREMIITSRFILRSGARRLDVITEFENTCK
                     HHRLRVVFPTRLNCDRTDAEIAYDVIGRDIHIKESSPYYGHPNPQYPMQRFVDMTDGE
                     AGFAVINNSGLREYEAMDQTDRPLAITLLRAFTYRNSPVFGRWETYPDMELAQCIGKF
                     EWRYALYPHAGDWKNGCYREAEDMNLPLEPAQAGPHEGTLPKAMSFLTLEGDNLQITA
                     LKQAEDRPGSYILRLFNPDDAPVQGRVILFKDVQEAWLTNLNEERQEALQPEKNRLML
                     EVGAKKIVTLEFRI"
BASE COUNT         1618 a         1936 c         1830 g         1373 t
ORIGIN      
        1 caaaacgtcc ataacctgat taagattcct gccttcgcag gaatgacggg gggagaagca
       61 ggaatgacgg ggggagaagc aggaatgacg gggagtgcag ggacggtgga gagcgtggtg
      121 acggcggtgc gtacaggcgt ggcgccggag acatgcaatt cttccctggg aacgccaatc
      181 tccgcattgg cagggacttt tcaggtctgt gatgttgtgt gaatgccgca ggacatcgcc
      241 cggctgcgta tgtgaataca gggggccgcc accctgcgca ctctccgatc cgaagccccc
      301 tacagaagcg gcgagatcag atggctgaga ttttccgccc aggcacggcg ccggctcact
      361 tcccgggggc ggatcatgtg gcaggtcagc tgaatctggc gcagccaggc ttcgagaata
      421 tcgatatcgg caggcgagta atggaacaat gcaatttcga aattcagata gatgctgcgc
      481 atgtccagat tcggtgatcc ggtaatggcg atcttgtggt caaagagcat gactttgcca
      541 tggatcatcg gtgcagggca aacataaata gcagcccctg ccgcactgag ttcgcgcagg
      601 gcaggccctc tcgccagatc ggcaatgcca tgattggaac gaagaggcac aataatccgg
      661 atatcgcaac cggaacgtgc cttcaatgca agcggtttca gcaaagatgc atccggcaca
      721 aaataagggg tcatgagcca gatacgcttt tccgcctcca tgatcgccgt aagcagcgca
      781 tcgtgaaagg tatcttcagg cacatccggc ccgctgggca ccacctgaat catacaatca
      841 tccaccgcat gaaaaggcgt cacgccttct atcatggcat tgagaaacgg ctcaggcgtg
      901 cgtggaaaaa ggcagccctc aggaagatgc agctgttcat cggcggcaaa ttcccagtcg
      961 tgccggaaca cctcttctat atcgtaaaca gcgggaccat acagaaacac agagtcgtcc
     1021 acatagcggt agcagtccgg ctcggcaccc ataaaaagca ttgccagatt catccctccg
     1081 acaacagccg ccacatgatc caccaccaca atcttccggt ggttgcgcag gttggcagac
     1141 catttccgct gcagcggcag taccggaaga aagcgtttgg cacggccacc ggcatcacga
     1201 atcggctgca caaaaccgcg gctggttgtg agacagccga ggctgtccag aagcaggcgc
     1261 acctccaccc cttcccgcgc ctttttcgca agcgccttca caacggcacg tcccgtgtcg
     1321 tcatcgccca gaataaatgt ggaaacgtgt atggaaacgc gcgccgcttc aaacagctgc
     1381 atcatggcgg cataagccgt ttcgccgctg ctgtgaaata ccacaaggtt acccggccgg
     1441 ggtgatggga aacctgcctg attcacaatg cgccctgccg ccggaaccag catctcctgc
     1501 ttttgcccgg ttccgggcac atagagccgc tcaatcatgt gaatgctgcg attgaatttc
     1561 ctgccgccga tgaccaggta caggggaacg ccgatatagg gggcaaggac gattgccatg
     1621 agccaggcga gggtgctgcc cgggggacgc ttttcctgca ggatccttac tgccagcagc
     1681 agtgccagca gaaacccgac gaccgtcaga aggtggtcga taatcagttg caggacattg
     1741 atgataatag gcttattcct ttggctctaa aagaacgaac gtgggcaacc ccagcacctt
     1801 ataatattca accacatcgc gggtcggaac actctgaagc gactcagcct gatattttat
     1861 tttaacataa ccgtcaagag ccgtcagcac atccggatca ctcagtaccg tatggctcat
     1921 gactgtacaa tttttgcacc aggtcgccca gaagtcaagc aaaacgggct gattcttttc
     1981 cagtgcctgc gccagccctt cctccagctg atgtacccag ataccattct catcgctttt
     2041 tatcgcaggc ggcgtcatga gactgtatgc cagatacccg taatacagcg caaatgcgat
     2101 aataaaaacg ccgaaggcct gcttcacccg aaccatccac atgcccggct tcggaagaag
     2161 cgacaaccct gctcccagaa aaggccaggg aagtgccatg ccgaccccaa gcccgaaagg
     2221 aagtgccagc gcaaacacat tgccctgact gtacagatcc tgcgcctgca gaatggtata
     2281 gatcacgaca ggcgccacgc aggcacccgc cagaagcgcc gacactgcgc ccatccccaa
     2341 agctgcaaag aaatgaccgc cttctttcgg agtcattccg agtctggtct gaaagcggga
     2401 aaaatctatc tgaatcacgt caaacatcgc cagcccgagc acaacaaaaa gaacagcgat
     2461 agcggcgtta aaccagaccg tggaattgat cgtgccgaaa gcgcttgaca gccccagcac
     2521 caccagaagt ccgagagagc catagaccag cgcaatgccc agcccgtagg caccgcccag
     2581 agcaaagcct ctgctgcggg atcctgccct ggtaccggca ccgataatgg caatattgat
     2641 cggaatcagc ggaagcacgc agggcgtgaa attcagcaga agcccgccgc caatcacaat
     2701 ccacaaaagc cagaggccgc ccagccctcc ggcattgctt ccttccgcat cggaacggtc
     2761 accggcaata aaagaaagaa agccttcttt gtccgtataa cccgccagcg tattcacaac
     2821 ccggaatttt tccgcaagcg catgccagtc cgcagagaga gcgggcggtt cctgctccgg
     2881 tatcgccgca ctttcttccg gcgtttcggc cggaggcacg gcgttcacat ccgtcaccgc
     2941 ctgcatggcg gacagaggcg cacggtcgaa ccagtcaggc agtgcttcct ctgcggctcc
     3001 ggcactttcc cctatcttca cagaaaaggg gatggattcg ctacggggcg cggcgcacat
     3061 ggcattgttg cacgcctgaa acagcaggga cagattcagc gtatactcac ccgctgccag
     3121 cgtcgcatcg gtttccattt caaaagcgag aaaaaaaact tcttcataga ccgccagcgg
     3181 ctcaggtgaa aaatccagat tgaccgtttt cgcctcagga tatgccacac cgcgaagcgt
     3241 aaagcctgtc tgcttctcaa actgcagttc ggtagcaatc agaaaaggat ccagaggctc
     3301 gtgggcgttc acatgccatc cgtcattcag catgaccttg accgcacccg taaagacgct
     3361 gcccggagca atcacggcgg ggcgtgacgc tatatcaaaa gagacttccg ggccgatcgt
     3421 ccctgctatc atctgtcctc gtgcagcagc acagagggtc atgcatgcgg caaaaatgag
     3481 agagaatcgt ttcataatct gttccttgaa aatcgcgcac ttccttttgc gaaacactct
     3541 tcatataagt ggtaacaaca cttacacggc gattagtccc gatcagaaag gcgggctttc
     3601 agcaacagac ggatgcgatt gctctcctaa atccgaaatt ccagcgtcac aattttcttg
     3661 gcgcccacct ccagcataag ccggttcttt tcaggctgaa gcgcctcctg ccgctcttca
     3721 ttcagattgg tcagccaggc ttcctgaaca tccttgaaga ggatcacacg gccttgcacc
     3781 ggtgcatcgt cgggattgaa aaggcgcaga atatagctgc ccggccggtc ttccgcctgc
     3841 ttcaatgcgg tgatctggag attgtcgcct tccagcgtca ggaagctcat cgccttcggc
     3901 agcgtacctt catgcggtcc tgcctgcgcc ggctccagag gcagattcat atcttccgct
     3961 tcacggtaac agccgttctt ccagtcgccc gcatgcggat aaagggcgta tcgccactcg
     4021 aatttgccga tgcactgcgc caactccatg tcggggtagg tttcccagcg cccgaagaca
     4081 ggagagttgc gataggtaaa ggcacggagc agcgtgattg ccagcggccg gtctgtctga
     4141 tccattgcct cgtattcacg cagccccgag ttattgataa cggcaaatcc cgcctcgccg
     4201 tccgtcatat ccacaaagcg ctgcatggga tactgcgggt tcggatggcc gtaataggga
     4261 ctgctttctt taatgtgaat atcccgcccg atcacatcat aggcaatttc cgcatcggtg
     4321 cgatcgcagt tgagccgggt cggaaaaacg acccggagcc ggtgatgctt gcatgtgttt
     4381 tcaaattcgg tgatgacatc cagccgcctg gcaccggaac gaagtatgaa gcggcttgta
     4441 ataatcattt cccggcgttc tttcgtcctg ccggtattgt tgagatcgcc ctgccggaaa
     4501 tcgacataag gttcatcttc cacactcacg ggaatctgca tatgataatc cacacgcatg
     4561 cgtgcgtaga gcggtcccga ttcttccagt gtgatgtaac agggagcccc gtgggacgta
     4621 atgatttcat cgcgctccgg tgccatgtgc acccaggaat gccccgtttc tccgccgtct
     4681 tccagataat gcagtcctga atacacccgc cccgtttcct tgtgtgtcat atccagcgtg
     4741 ccgttggaat tgaagaccac acgcaggtgc tcattttcca gcacattggt ttccggcacg
     4801 agagagcccg gcaggtattc aaaaccgggg cgtcgggtca catgataggt gctgtaaccg
     4861 aaagcgggaa tgtcttcagt ttcaagatgg cagcggacac gctccgtccg cagctcactg
     4921 gagatatcct gaaggttgcg cacgagcacc cccatgggat actgctcttc acgctgcaca
     4981 cgaagggact ttccttcggg cgtctgcaca ccgaacgccg tatagcccat attctgcggc
     5041 atatccacaa gacaggaaat cacagcgcgc cgcgggaagg ggcagggatt gaaaaccgta
     5101 atcacgctgt cccggggcga caggtcgctg ttgtccacct gaatctgcac atgctgcagc
     5161 ccgcgccggg cgacaccttc ggaaatcagg ttaatctggt tgaagcggta aatggaatcc
     5221 tcttccatac gatccactcc ggcaccggtg atggtatcgt ggggatgatt tttaagcagc
     5281 agcttccagc agcgtgccag ggctgtttta tagtactcgc cctccagcat ggaagcgatg
     5341 gcggaccagg gctccgcccg ccgctgcaga ttcacttccg ccttgtgatt gttctgcttg
     5401 agccggggcc gggcactgat aatatcaccg aaaagatgcg tccagctgcc cgtcgcaccg
     5461 ggatcacgga attcgccgcg caggacaatc ggatctttca cctcttttgc catggcggtc
     5521 atgtattctt tcagcgtgga aaaacggatg tcgtgctcgg gaagcaactc ctgacagagc
     5581 ttcatgatcc ggctctcctc cggatcgggg ttggaggtat caaagccctg catacataca
     5641 atctggctgg tagtgaaatg ctctgattcg tcccggacca gtttcagcag ctgttcgcgg
     5701 atgggcgcat cattccactg ttttctccca tcatcgagaa tatagtagtg atcccggagc
     5761 cgctcgctgg cgatcatacg gaaaggagcg gcgccgcggt cccagtcata gcgctgatac
     5821 acatcatccg agccgtaacg cagcacacga taaatgtaaa tgtaataact gaagcggctc
     5881 atgcagccga agcgggaacc cagcagccgg gaaccgtcgg ggccttccag aatgtattca
     5941 catttcggcg tattaatccc ccggtaaaaa atgatgtcgt caataccaaa accccggtaa
     6001 atctgcggaa tctgggaggt ttgaccgtaa ctgaagggcg tgtatccgct tttcgtcaca
     6061 ccgcccagcg cctcggcgct ccggtgcccc acaacaagat tgcgcaccag cgattcgccg
     6121 ttgcagacat attcctcggg cagagaatac cagggtccga taaggagacg cccgcttttc
     6181 acatgcttct caatggtcgc acgcttttca gggcgaagct ccagataatc ctccacgcaa
     6241 agggtctgcg agtccatggt aaaggacgtg aaatccggat ccgtgtccag aaggctcagc
     6301 agctcatcca tgaacttgag cagaagtaat cgggtctctt caaagggata gccccattcg
     6361 cgatcccaat gggaattgga gacaacatgg atggtttttc tttcctgagc cataagctga
     6421 aattcctgta ggttgaaagg gaaagggggg agtgcatttc tgcgctgcta gtatgcataa
     6481 acaggcggat cagttacaaa ttatgtacaa gcaaagcagg cagcaatgag agattaagac
     6541 ggaaacgggc ttccgggagg gagaggacat cacacagggc ttagacagag cacggggcgt
     6601 tttgcagccg tgtccgttga tcaggttggt tccaaggaca gcgaccaggc gggaaccggg
     6661 caggaggcag gcgggcggac gcgtgtccgt tgatcaggtt ggttccaagg acagcgacga
     6721 cgccgctgaa ggagctgcgc gccaggctag gcggtgt
//
LOCUS       DAOPWB010000160         6666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_11447_length_6666_cov_4.835728, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000160 DAOPWB010000000
VERSION     DAOPWB010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11447_length_6666_cov_4.835728"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PKN92_11670"
     CDS             <1..751
                     /locus_tag="PKN92_11670"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ49492.1"
                     /translation="ITSPDAAEHVLQKASPLDGVVHCAACQTGDAEALMKTNYFGTKQ
                     LLEAARKAGASRFIYLGSMSYLRRPLELPVTEVHPLDMAGPYAASKYLGEWIVLQEEK
                     ELKGISLRISSPVGAGLKYRRIFRFFVEKALRNEDILLHGQGGRRQDYVDVRDVAEAV
                     QLALTKDTGRGAALFNIASGRALSNLALAERCIAVLNSGSEIRFSGEEDPDENMAWQI
                     SIDRAEAQLNYVPRFSIDDSILALAGELEQA"
     gene            complement(748..3135)
                     /locus_tag="PKN92_11675"
     CDS             complement(748..3135)
                     /locus_tag="PKN92_11675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ49493.1"
                     /translation="MDEKNSLDNTAAAHDVVPENADAPLPSRGIILSHNRKGRAYGIT
                     CLKKWMRLNTTLFKVEKVDVAFSKGSVDEWTELLEFAKTEDIRLSLRIQNLQDDTFFD
                     ALPELELLDILLAPETLELGALSKWISRAQEHKIPVRVLLPLTMRSLPGLEESVACFK
                     AADAVSFTWEKHFSGGRKKFRTPSSESLQWMNSLTRRLNREKVDVTLLNIPYCLVDED
                     NHGNVLCGEELFRDHSQYLYASWAVAKRIFLFGHARASLILDEFLASRQGRRNLADNL
                     LLPWVFGKVRIEGRLWLLRRLSRYISPDWIAVQREKEHAKRHKLLPVASWEKTVRHDA
                     CTGCRFYRLCIGNTALFEKGFSSLNVTPFGGTPLLTTATVPSQRHRWFDVIDQKRRHF
                     HEHQRALAEEALRISRATPPSKEMTVLDYAIEDHYNHWDGGDLKRWFSLSRGEMISTV
                     LARITPPFTLMVTFGGGIAEQIGFSFGRHAKIVCPMIDYSHKLTLHVDKDGYYALLRD
                     GVLVQPTEFSGASRVPERLPGILEPRISMHNVDGYVLTTTLLLWDQEAKAKQDLSRIK
                     YSVIIVSVRYARRLQAALLSLAHQKNFPLDALEVVLAYVPGADGAEDLIDAMECRFPQ
                     LRIVRVPFPKEYVRSKGFMINESLHAASGEWITLLDADIVLPPDIFARVDAVSEKSHF
                     IAPDGRYMLSPDTTHAILLGERKPWEEYDAIAAEAIETRHHEAKGYPLGFFQCIRRDI
                     LEKVPYQEFNHFEGADWFFSKEVIDRFGDATRLSGVYVLHLDHEGRQWYGTEKHR"
     gene            complement(3153..5252)
                     /locus_tag="PKN92_11680"
     CDS             complement(3153..5252)
                     /locus_tag="PKN92_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ49494.1"
                     /translation="MNPIITLEQVTKRFPPRRGALGLRGRGSLGDLLRGTKAEDFTAL
                     ENISMEMHPGESLGIIGRNGSGKSTLLSLIAGVTLPTSGRIQVFGRVASLLELGAGFN
                     PVLTGRENIYLNAGLLGMRHAQVDAVFDDIVRFSGIEEFIDQPVATYSSGMYVRIGFS
                     VAAFVNPDIFLADEVLAVGDEDFQRKCRRKIGELREQGKTILFVSHDLSIVNTLCERV
                     ILLDGGRIIQRDTPQKTITYYLRQVGRDKGVHTFSDGDLEAIHCDGRISLFHKQEEMT
                     APSGFTMNIESLGRHHSSADAEWTVVERDSRGCRAEGRMMRLPVRLIWTLKVEKGVFH
                     WQAALDVEQRCPLSRIDVECYLPAAYGTWLCGHMSGTFPDIQPGDITWNVVAAPETSA
                     SSGAALPATDSPLPAVVFQFNKTNPHFGLFWANTDYMSACRVLDYTARFPDSQENFAP
                     GVHPLLELRLDTTVAAAEILDHLLARESLDCGRLKARFEQGRIRLLWDKEPLTAFLHV
                     YSSMLIAHLWNDSQNFAWQEPEPLGTGLRIVGKSRRFPFAQEWELIPRENALGIRVWL
                     HAEEELDVQEYHFSLVLPKSYTSWTTDREKGVFPDFEPEAGHWVHLNRNYESGVAMTA
                     ESPSLPMLTLCTDDPELPLRQTVLNTSFSENSRVLQALRPSGQSGLRFAPGRHLYFSG
                     AIKVTPQKETDPSAVQA"
     gene            5519..6619
                     /locus_tag="PKN92_11685"
     CDS             5519..6619
                     /locus_tag="PKN92_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49495.1"
                     /translation="MHPRAKNILFNFAALLIGCIPVLLMLAAGELYLRCRSSAVPRMR
                     DVIEHNLSDFSIPDPVLGMKLLPGYEGAHHVKVNGSEVFSAEYRIDEEGRRITPVEHP
                     ESRDKAALFFGCSFTFGQGVEQDETLPACFGRECESLLPVNYAVGGYGPQHLWLQMND
                     PEFLRRVPRERGVVIYGFFNDHINRLLGNKRMIDDWGGWLPWLSFEDGRVVKHGFMGD
                     RDRNEQGTLNFLKSFHMGRFILKRLGLLQAKQFTDEEGMDGLVAFLLLIKEKIAEILP
                     GYQFVVFSYPWTFKVEGLTERMQAAGIPFFDYTHAYPDQNANQDRYFYRDGFRAVWGH
                     PKAIVYADVAKWLAQDLAEYCGGDEKTIGDAL"
BASE COUNT         1470 a         1732 c         1932 g         1532 t
ORIGIN      
        1 cattacctct ccggatgctg ctgagcatgt gcttcagaaa gcatcgccgc tcgatggcgt
       61 tgtgcattgc gccgcctgtc agacaggcga cgccgaggca cttatgaaaa ccaactattt
      121 cggcaccaaa cagctgttgg aagcggcgcg aaaagcggga gcttcccgtt ttatctacct
      181 gggcagcatg agttatctcc gtcgcccgct ggagcttcct gttactgaag tgcatccgct
      241 ggacatggcc gggccctatg ccgcctcgaa atatctggga gaatggatcg ttcttcagga
      301 agaaaaggag ttgaaaggga taagccttcg gatttcttca ccggtgggcg caggactcaa
      361 gtaccgtcgg atttttcggt tttttgtgga aaaggcgctc cgcaacgagg atattctact
      421 gcacgggcag ggcggcaggc ggcaggatta tgtggatgtg cgggatgtgg cagaggcggt
      481 gcagctggcg ctgacgaaag acacagggcg cggtgcggcg ctcttcaata tcgcatcggg
      541 gcgtgccttg tccaatctag ccctggcgga gcgctgtatt gccgtgctga actccggctc
      601 ggaaatccgt ttttcgggcg aagaagaccc tgacgaaaat atggcgtggc agatcagtat
      661 tgaccgcgcg gaggcacagt tgaattatgt gccccgattc agtattgacg acagtattct
      721 tgctcttgcc ggggaattgg agcaggctta gcggtgcttt tccgtgccgt accactggcg
      781 cccttcgtga tcgagatgaa gcacatagac accgctcagg cgtgtggcgt ccccgaaccg
      841 gtcaatgact tccttggaga agaaccagtc cgcgccttca aaatggttga attcctgata
      901 aggcaccttc tccagaatat cccgtcggat gcactgaaag aagcccagcg gatagccttt
      961 tgcctcatga tgcctggttt caatggcctc ggcggcaatg gcatcgtact cctcccaggg
     1021 ctttctttcg cccagaagga tggcatgggt cgtgtcgggc gagagcatgt atctgccgtc
     1081 gggcgcaata aaatgactct tttcagaaac agcatccacc cgggcaaaga tatcgggggg
     1141 gaggacaata tcggcatcga gcagggtgat ccattcgcct gatgcggcat gaagggactc
     1201 attgatcatg aagcccttgg agcgtacata ttctttcgga aagggcaccc gtacaatacg
     1261 cagctgcggg aagcggcatt ccatggcatc gatgagatct tcggcaccgt ccgcaccggg
     1321 cacataggca agtacgactt cgagcgcatc gagcggaaag tttttctgat gcgccagaga
     1381 aaggagtgcc gcctgaagtc tgcgcgcata ccggacgctc acaataataa cggaatattt
     1441 aatacggctg agatcctgtt tcgcctttgc ttcctgatcc cacagcagca gtgtcgtggt
     1501 cagcacatag ccgtccacat tgtgcatgga aatgcgaggc tccagaatgc cgggcagccg
     1561 ttcaggcacc cgggaagcgc cggaaaactc tgtaggctga acaagtacgc catcccgcag
     1621 cagggcataa tagccgtctt tatccacatg cagtgtgagt ttatgggaat agtcgatcat
     1681 ggggcagacg attttcgcat gcctgccgaa actgaatccg atctgttctg cgataccgcc
     1741 gccgaaggtg accatgagcg tgaaaggcgg tgtgatccgg gcaaggacag tactgatcat
     1801 ttctccccgg gagagagaaa accagcgctt gagatcgccg ccgtcccaat ggttgtaatg
     1861 gtcctcaatc gcatagtcca gcacagtcat ttcttttgac ggcggggttg ccctggaaat
     1921 gcgcagcgct tcttcggcaa gcgcacgctg atgctcgtgg aaatgccggc gtttctgatc
     1981 aatcacatca aaccagcgat gccgctgcga gggtaccgtt gctgtcgtca gaagaggcgt
     2041 gccgccgaac ggggttacgt tgagtgatga aaatcccttt tcaaaaaggg cggtattgcc
     2101 tatgcacagg cggtaaaagc gacagcccgt gcaggcgtcg tgcctgacgg ttttctccca
     2161 ggaggcgaca ggcaggagct tgtgcctttt tgcatgctct ttttcgcgct gcaccgcaat
     2221 ccagtccggg cttatataac ggctcagccg ccgcagcagc cagaggcgcc cctctatccg
     2281 tacctttccg aagacccagg ggagcagaag attgtcggca aggttccgtc ggccctggcg
     2341 ggaggcgaga aattcgtcca gaatcaggga cgcccgggcg tgaccgaaga gaaaaatacg
     2401 ctttgccacc gcccaggaag catagaggta ctgactgtga tcccggaaga gttcttctcc
     2461 gcacagcaca ttgccgtggt tatcctcatc gacaagacaa taggggatat tgaggagtgt
     2521 tacatccacc ttctcccggt tgagccgacg tgtcaggctg ttcatccact gaagggactc
     2581 ggaagaaggt gtcctgaatt tctttcttcc gccggagaaa tgtttttccc aggtgaaaga
     2641 gacggcatct gccgccttga agcaggcaac gctttcttcc agcccgggca gagagcgcat
     2701 tgtcagtggc agcagaacgc gtacaggaat tttatgctcc tgtgcccggc tgatccattt
     2761 gctgagcgcg cccagttcca gtgtttcagg agctagcagg atgtcgagga gttccagttc
     2821 aggcagtgcg tcgaaaaagg tgtcgtcctg cagattctga atgcgcagcg agagccggat
     2881 gtcttccgtc ttcgcaaatt ccagcagctc tgtccattcg tcaacgcttc ctttgctgaa
     2941 ggcaacatcc actttttcaa ctttgaacag tgtggtgttg aggcgcatcc attttttcag
     3001 acaggtgatg ccgtatgccc ggccttttct gttgtgggaa aggatgatgc cccgggaagg
     3061 cagtggtgca tcggcgtttt ccggcacgac atcgtgagcg gcggctgtgt tgtccagact
     3121 gttcttttca tccataggtg cattttacct tgttacgctt gtacagcgga agggtctgtt
     3181 tctttctgtg gggtcacttt gatggcaccg gagaaataaa gatgccggcc cggcgcaaag
     3241 cggagtcctg attgtccgga ggggcgcagc gcctgcagga ctctggaatt ctccgaaaaa
     3301 ctggtgttga gcacggtctg gcgcaaggga agttccggat catcggtgca cagggtcagc
     3361 atcgggagag aaggactttc tgccgtcata gcgacgccgc tttcatagtt cctgttcaga
     3421 tgtacccagt gccccgcttc aggttcaaag tcgggaaaca ctcccttttc ccggtccgtt
     3481 gtccaggagg tgtagctctt cggcagcacc agactgaagt ggtattcctg tacatccagt
     3541 tcctcttctg catgaagcca gacccggatg ccgagggcat tttcacgggg gatcaactcc
     3601 cactcctgag cgaaggggaa acgccgtgat ttgcccacga tccgcaaccc tgtgcccaga
     3661 ggctccggct cctgccaggc aaaattctgg ctgtcattcc agaggtgcgc aatcagcatg
     3721 gagctgtaca catggaggaa tgccgtgagc ggttctttat cccagagcag acggatgcgg
     3781 ccctgctcaa aacgcgcttt gagtcttccg cagtcgagac tttctctcgc aagcagatga
     3841 tccagaatct cagcggcagc cacggttgta tccagacgga gttccagaag gggatgcacg
     3901 cccggtgcaa agttttcctg tgaatcaggg aaacgtgcgg tgtaatcgag cactctgcag
     3961 gcactcatgt aatcggtatt cgcccagaaa agcccgaaat gggggttggt cttgttgaac
     4021 tgaaagacca cggcaggcag aggtgaatct gttgcgggaa gggcggcgcc gctgctggcg
     4081 cttgtctccg gtgcagccac cacgttccag gtgatgtctc ccggctggat atccgggaag
     4141 gtgccggaca tatgaccgca gagccaggtg ccgtatgccg caggcagata gcattccaca
     4201 tcgatacggg aaagagggca gcgctgctcc acatccagtg ctgcctgcca gtggaagaca
     4261 cccttttcta ctttcagtgt ccagatcagc ctgaccggca ggcgcatcat tcgcccctcc
     4321 gcacggcagc cccggctgtc ccgctccaca acggtccatt ccgcatcagc actgctgtga
     4381 tgcctgccaa gcgattcgat gttcatcgta aagcccgaag gtgccgtcat ttcctcctgc
     4441 ttgtggaaaa gggagattct gccgtcgcag tggatcgctt ccagatcgcc gtcggagaat
     4501 gtgtgaaccc ctttatccct gcccacctga cgcaggtaat aggtgatggt tttctgcggg
     4561 gtatcgcgct gaataattct gcctccgtcc agaaggatca ctctttcgca gagggtattg
     4621 acaatactca ggtcatgaga aacgaaaaga atggtcttgc cctgctcgcg cagttcgccg
     4681 atcttccggc ggcatttgcg ttgaaaatcc tcatcgccta cggcaagcac ctcatcggca
     4741 agaaagatgt cgggattgac aaaggccgcc acagaaaagc cgatgcgcac gtacataccg
     4801 cttgaatagg tggctacggg ctgatcgatg aattcttcaa tcccggaaaa gcggacgatg
     4861 tcgtcaaaga cagcgtccac ctgggcgtgg cgcataccga gcagtcccgc attcagataa
     4921 atattttcac gtccggtgag caccggattg aagcctgcgc ccagctccag aagagaggcg
     4981 acgcgtccga aaacctggat tctgccgctc gtcggaagag tgaccccggc gatcagactc
     5041 agcagggtgc ttttccccga tccattgcgg ccgataatgc ccagcgattc accggggtgc
     5101 atctccatgg agatgttttc cagcgccgtg aaatcctccg cctttgtgcc gcgaaggaga
     5161 tcgcccagac tgccgcggcc ccgcagcccc agcgcaccgc ggcggggcgg gaaccgtttg
     5221 gtgacctgtt cgagagtgat aatgggattc atgtaaaaat gcgccctttg ctggtgagta
     5281 cggcgggtcg ccgctgaccg gctcttttat tgaaagccag ccaatttctg agaaaacagc
     5341 cttttcctgc cgtgccgacc cgggaaaatc atacctgatt cagtgcggga aagcaaaggg
     5401 ggaacggcac agaaaggggc agttattccc cgcaagaaga agcggaaggc atccgggaac
     5461 atatgaatct cccgaatcat ttttgttatg atccactgat tacacctaag gaaccgccat
     5521 gcatccccgc gctaaaaata tactgttcaa ttttgctgct ctgctgatcg gctgtatccc
     5581 tgttttactg atgctggcgg ctggagagct gtaccttcgc tgtcgaagca gtgcagtgcc
     5641 gcggatgcgg gatgtgatcg aacataacct cagtgatttt tcaattcccg atcctgtgct
     5701 ggggatgaaa cttctgccgg gctatgaagg ggcgcatcat gtcaaggtca atggatctga
     5761 agttttttcg gctgaatacc ggattgatga ggaagggcgc cgtattacac cggtagaaca
     5821 tccggaaagc agagacaaag ctgcgctttt tttcggatgc tcctttactt tcgggcaggg
     5881 cgtggaacag gatgaaacgc tccctgcctg cttcggaagg gaatgcgaat cccttctgcc
     5941 tgtcaattat gcggtaggcg ggtatggacc gcagcatttg tggctgcaga tgaacgatcc
     6001 ggaatttctg cgtcgggtgc cccgggagcg tggtgtggtg atttatggct tttttaatga
     6061 tcatattaac cggctcctgg gtaataagcg catgatagac gactgggggg ggtggcttcc
     6121 ctggctcagc tttgaggacg gacgggtggt gaagcacgga ttcatgggtg accgtgaccg
     6181 aaatgagcag ggtacattga attttttaaa atcttttcat atgggaagat ttattctgaa
     6241 gcggctgggg ttgcttcagg caaagcagtt tacagacgaa gagggtatgg atggattggt
     6301 cgctttcctt ctcctgatta aagaaaaaat cgctgaaatt ttacccggct atcagtttgt
     6361 cgttttttcc tatccctgga cctttaaagt ggaaggtctt acagagcgca tgcaagcagc
     6421 gggtattccc tttttcgact atacacatgc ctatccggat cagaatgcga atcaggatcg
     6481 ctatttttac agagatggtt ttcgcgccgt ttgggggcat ccgaaagcaa ttgtgtatgc
     6541 cgatgtggca aaatggcttg ctcaagatct ggctgagtac tgcggcggcg atgagaaaac
     6601 aataggcgac gctctttgaa aggtgtggtg tttcgccgtt cgggaaagga attgccgtga
     6661 ctgatc
//
LOCUS       DAOPWB010000161         6571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_11643_length_6571_cov_5.035298, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000161 DAOPWB010000000
VERSION     DAOPWB010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11643_length_6571_cov_5.035298"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2038)
                     /locus_tag="PKN92_11690"
     CDS             complement(<1..2038)
                     /locus_tag="PKN92_11690"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNZ49496.1"
                     /translation="MPLLLLAVLCAHSVIDLHPAPQHADFYDKRYCALARELVLVVQA
                     DARADFLELFSPISNSLGFTPALSPAGRRQNGTALHAGTVENTRNTGHKELKKYLSQV
                     SKMPPGGYVLEVTKKQICLLGTDRSGLINGIQTLVQMIEESKKLGLAIEGVPVIPCAF
                     IRDYPDTALRAAWVQGALARPQLESYAALKCNMLFFESDDFYDLDSDAVLQWQERFRL
                     ARQLGMTPVPVFDFFNAGVNLLKKYPAAVEGRARVDHLTMDGETWIPLSRTNLIYTET
                     SPIRVQSGNQWLDIRKDLQLQQGGLMPPYLDSMSRSWLVRPAPESGLPARAPLSITYS
                     HAPAGSNALCPHAPESAQAVRDALQKLIDGLQPEYLHCGGSRISRINQDLRCRDAARP
                     SGENWLKALTMIHSTLRDLKSPAEMILWADHLLPRPDQPADKASLDGIAAKLPGNPIL
                     FPRFVLYEGFPREALPFTLQWMKQRDMPLNLALSGPAPHIQAALQQQAEQKGRSAGVC
                     VLDQDPHSLRVRTAFNRAWSAAGVRRVWPEALNHFFDAVLWTPSFEEIKKAQMVYLDR
                     EILKGEKPADLRARFKGFLGSLPGELQPRENPETAQAQSLFDLLTRYLEEEFTFSSGK
                     EQSALSALLKLIKQYEGIDPDMEPGRTDRIQETVKRQKRFVPASILFGESLAYYR"
     gene            complement(2055..2918)
                     /locus_tag="PKN92_11695"
     CDS             complement(2055..2918)
                     /locus_tag="PKN92_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNZ49497.1"
                     /translation="MSVSSFLKSRSLRMESALEAALDRCAGAPPELMEAMRYSLLGGG
                     KRLRPALALTACELICGTEEPALPAACAVEMIHCYSLIHDDLPAMDNDDIRRGKPSAH
                     KRFSEATAILAGDALQTLAFEILSDTRDIRQIVTLAKAAGAEGMAGGQILDMTAEKKR
                     LTLEELQHLHACKTGALIRASLELGALAANADEKQLAAFTSYGTHLGLLFQITDDILD
                     VEGDAAVLGKSVGKDALSEKMTYPQLLGMEEARRLAEDAANKARQTLELFGERAHILR
                     NLVLYILHRNH"
     gene            complement(3080..3298)
                     /gene="xseB"
                     /locus_tag="PKN92_11700"
                     /pseudo
     CDS             complement(3080..3298)
                     /gene="xseB"
                     /locus_tag="PKN92_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            complement(3295..4656)
                     /gene="xseA"
                     /locus_tag="PKN92_11705"
     CDS             complement(3295..4656)
                     /gene="xseA"
                     /locus_tag="PKN92_11705"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNZ49498.1"
                     /translation="MLTPDTKIFTVAQLTREIKTLLESQIGTVWVSGEISNWSRAASG
                     HAYFSIKEEGSQIDAVIFKARLNRLGLTPENGLEVLAKGEITVYAQRGRYQLVITELH
                     LKGLGALQIAYEKLKKKLAEEGLFDEKHKKKLPLLPRRIGIVTSPSGAAIRDILNVIR
                     RRFSNVHILIYPALVQGSGAAAEIAAGIRVLDSMQVDVMIVGRGGGSLEDLWAFNEEA
                     VVRAIFAAKTPIISAVGHEVDVTLSDLVADLRAPTPSAAAELVVQEQESLFDQVKQKE
                     HRLERAVAHLLEQAQSRLERHSGHYLFRNPERLLQPYQQYCDELQGALQRLGKAVLES
                     AKNRVDRSGQSLKHLSPQHRLVLQRQRLHELIAAFRRLGTRMTLPYRSSLAPLLAQLN
                     SMSPLAVLSRGYALVWKMPDHSLIRKAETLQIDDEITARFASGEIKAKITEITGEQEN
                     HLS"
     gene            complement(4791..6269)
                     /locus_tag="PKN92_11710"
     CDS             complement(4791..6269)
                     /locus_tag="PKN92_11710"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated pathogenicity-related family
                     protein"
                     /protein_id="HNZ49499.1"
                     /translation="MRSLFSASSFVLLLSLIFFSGNAPADGSYAHGRAKEGLTALDRY
                     VAKEDNNFKWERVLTEKEDGITCHTLSMVSQQWRHKGEVNRLLWEHWITVCVPDTVTH
                     PTALMFIDGGSNREGEEPRTADSTIKNLAKQSGCVTSTVYMIPNQPLVFWDQMGMGRG
                     EDALIAYTWLKFYQTGDEEWPLRLPMTKAVVRAMDAVQAFAASDAGGGNQVKDFIVAG
                     GSKRGWTTWTTAIVDARVRGLVPFSIDLLNVPASFRHHFEALGRWSVAVGDYLALDIL
                     KWLETPENKALMAIIDPYSYVDRLNIPKLVVNAGNDQFFLPDSSQFYWNDLQAPKWLR
                     YMPNVGHGLNRTEAYNSLASFFIANARELPIPNYSFSFEGDNRLKAQLLPASNGDIVQ
                     PSRVTLWEAWNPKARDFRGNTCPYVPREIEATADGIYEVAIDVPAKGYKACFLELTFP
                     GPLEEIPYTFTSGVRILPDVPTKKYTPGTPPKGFITGASTEN"
BASE COUNT         1428 a         1807 c         1825 g         1511 t
ORIGIN      
        1 ggcgataata agccagactt tctccgaaaa ggattgaggc cggtacaaag cgtttttgcc
       61 tttttaccgt ttcctgaata cggtcggtgc gccccggttc catatcaggg tcaatacctt
      121 cgtactgttt aataagcttc agcagtgcgg agagcgcaga ttgctccttc ccgctgctga
      181 aagtgaactc ttcttccagg taacgggtaa gcagatcgaa aagggactgc gcctgcgctg
      241 tctccggatt ctcacggggc tgcaattcac cgggaagtga tccgagaaag cctttgaacc
      301 gtgcccgcaa gtctgccggt ttttctcctt tcaggatttc ccggtcaaga tagaccattt
      361 gcgccttttt tatttcttca aaagaaggag tccacagcac agcatcaaaa aaatgattga
      421 gtgcttccgg ccagacacgg cgcacgcccg ccgcagacca ggcgcggttg aaggctgtgc
      481 gcacacggag ggagtgagga tcctgatcca gcacacacac tccggcgctg cgaccttttt
      541 gctcagcctg ctgctgcagt gccgcttgaa tatgcggtgc cggaccggat agagccagat
      601 tcagcggcat gtcccgctgc ttcatccatt gcagggtaaa cggcagcgct tcccggggaa
      661 agccttcata gagcacgaag cggggaaaga gtatgggatt gcccggtagt ttggcggcaa
      721 ttccgtccag ggatgctttg tcggcgggct gatcgggtcg ggggaggagg tgatccgccc
      781 agagaatcat ttccgccgga cttttcagat cacgtagcgt gctgtgaatc atggtcaggg
      841 ctttcagcca attttcacct gaaggccggg cggcatcacg gcagcgaaga tcctgattga
      901 tccggctgat ccggcttccc ccgcaatgca ggtattcggg ttgcagtccg tcgatcagtt
      961 tttgcagcgc atcccggaca gcttgtgcac tttccggcgc atgaggacag agtgcgttgg
     1021 aaccggcagg agcatggctg taggtgatgg aaagaggtgc tctggcgggt aatccgcttt
     1081 caggtgccgg gcgcaccagc cagcttctgc tcatgctgtc aagatagggc ggcatgagcc
     1141 cgccctgctg cagctgcagg tcttttctga tatcaagcca ctgattgcca gactgcaccc
     1201 ggatcgggct cgtttcggtg tatatcagat tggtgcggga gagcgggatc caggtctccc
     1261 cgtccatggt aagatgatcg acccgggcac gtccttccac ggcggcagga tattttttga
     1321 gaagattgac gcctgcgtta aagaaatcga agaccgggac aggggtcatg cccagctgcc
     1381 gggcaagccg gaaccgctcc tgccactgca ggacggcgtc actgtccaga tcgtaaaaat
     1441 catcgctttc aaaaaagagc atattgcatt tgagtgcggc gtagctttcc agttgaggac
     1501 gtgccagagc gccctgcacc caggcggcac gcaatgctgt atcgggataa tcgcggatga
     1561 aggcacaggg gatgacagga acgccctcta ttgccagccc cagttttttg ctttcctcaa
     1621 tcatctgtac cagcgtctga ataccgttga taagccctga acgatccgtg ccgagaaggc
     1681 agatctgttt ctttgtcact tccagcacat agccccccgg cggcattttg gatacctgag
     1741 agaggtattt ttttaattcc ttgtgccccg tgttgcgcgt gttttccacg gtgcccgcat
     1801 ggagtgccgt tccgttctgc cgtcgtccgg ccggagacag ggctggggta aagccgaggc
     1861 tgttgcttat gggcgagaaa agctccagaa aatccgcgcg tgcatcggcc tgcaccacca
     1921 gtacaagctc ccgggcgagg gcgcaatacc gcttgtcata aaaatccgca tgctgcggtg
     1981 ccggatgtaa gtcaatcacg ctgtgcgcac agagcacggc cagaagaagt agaggcatgc
     2041 cgtcaattcc cttttcagtg attgcgatgc aggatataga gaaccagatt gcgcaggata
     2101 tgcgcccgtt ccccgaagag ttcaagggtc tgacgtgctt tgttggcagc atcttccgcc
     2161 agacgccgtg cttcttccat acccaaaagc tgaggatagg tcattttttc gctgagagca
     2221 tctttgccta cacttttgcc cagcactgcc gcgtcgcctt ccacatccag aatatcatct
     2281 gtgatctgaa agagaagtcc cagatgggtg ccgtaactgg tgaaagccgc cagttgtttc
     2341 tcatcggcat tggcagccag cgcacccagc tccagagatg cccggatcag cgcacctgtt
     2401 ttgcatgcat gcagatgctg cagctcctcc agagtgaggc gctttttctc ggcagtcata
     2461 tcaaggattt gcccccctgc catgccttcc gcgcctgctg cttttgcaag cgtgacaatc
     2521 tgacggatgt cccgggtgtc gctcagtatt tcaaaggcga gcgtctgcag cgcatctccg
     2581 gcgagaatcg ccgtcgcttc gctgaagcgt ttgtgtgctg agggcttgcc gcgtcgtatg
     2641 tcgtcattat ccatggcggg cagatcgtca tggatcaggg agtagcagtg aatcatttcc
     2701 acagcgcagg cagcggggag agcaggctct tcggtgccgc agatgagctc gcaggcagtc
     2761 agggcgaggg cgggccgcag tcttttcccg ccgccaagca gactgtagcg catcgcttcc
     2821 ataagttccg gcggagcacc ggcgcaccgg tcgagtgctg cttccagtgc ggactccatc
     2881 cgcaaagatc tggatttcag gaaagaagaa actgacatca gaaaagatca cctgttgtat
     2941 cgtccggctc ctcttcagac cagaagtttt cttcgggttc gggaacgaca ggggcagcgg
     3001 gcggggcagt gcgccggggt gccggtgcct gagcgggtgc cggcacttca gattcttcag
     3061 acgcttcctc aaaatcttcc gtttcaaacc cgccgtccat ggtttgcacc agtttttcga
     3121 ttttgcgctc tgccgctttc aaggtttttt cgcatttccg ggtgagcgca atacccgcct
     3181 caaactgctt gagtgcctct tccaggggca gatttccccc ttccagctgc cttacaatcg
     3241 cttcgagttt ttccagatct gcttcaaagc ctggctgtgt ttttttctct gtgctcatga
     3301 gaggtgattc tcctgctctc cggtgatttc tgttattttc gcttttatct cgcccgatgc
     3361 aaaacgcgct gtgatttcat catcgatctg cagcgtttcc gcttttctga tcagcgaatg
     3421 atccggcatc ttccagacaa gtgcataacc ccgtgaaagt actgccagcg gactcatgct
     3481 gttcagctgg gcaagaaggg gtgccagact gcttctgtag ggaagagtca tccgggtgcc
     3541 gagccggcgg aatgcggcga tgagttcatg aagccgctgt cgctgcagaa caagacggtg
     3601 ctggggagaa agatgtttga ggctttgccc gctccggtca acccggtttt tcgcactctc
     3661 cagtacggct ttgcccagtc gctgcagcgc accctgcagt tcgtcacaat attgctgata
     3721 cggctgaagc agccgttcag gatttctgaa aagataatga ccgctgtgcc gttcaaggcg
     3781 gctctgcgcc tgctccagca gatgagccac ggcacgttca agccggtgct ccttctgttt
     3841 aacctgatcg aaaagagatt cctgttcctg cacgaccagt tcagctgccg cgctgggtgt
     3901 cggggcacgc aaatcggcga ccagatcgga gagcgtcaca tccacttcat gacccactgc
     3961 cgaaatgatc ggtgtctttg ccgcaaagat ggcacgcacc accgcttctt cgttaaaagc
     4021 ccacaggtct tccagagagc cgccgcctct gcccacaatc atcacgtcca cctgcatgct
     4081 gtcgagcaca cggatgcccg ccgctatttc ggctgcagcg ccggagccct gcaccaatgc
     4141 aggataaata agaatatgca cattggaaaa gcgacgccgg atgacgttca gaatatcgcg
     4201 gatagcggca ccggaaggag aggtgacaat gccgatgcgc cggggaagaa ggggaagttt
     4261 ctttttgtgt ttctcatcaa aaagcccttc ctccgccagt ttctttttga gcttttcata
     4321 ggcaatctgc agcgcaccca gtcctttgag gtgcaattcc gtgatcacca gctgataccg
     4381 cccgcgctgc gcgtataccg taatttctcc tttggcaagc acttcaagac cgttttcggg
     4441 tgtaaggccc agacggttca gacgcgcctt gaaaatgacg gcatctatct ggctgccttc
     4501 ctctttaatg gaaaaatagg catgacccga agcggcacgg gaccagttgg atatttcgcc
     4561 ggacacccag acggtcccga tctgcgactc gaggagtgtc ttgatctccc gggtcagctg
     4621 ggcgacggta aaaatctttg tgtctggtgt cagcatgagt gaattgtagc gaaaaaggca
     4681 ggataaaagc actcatcccg ccgaaggcat ctttcatgcg gcaggcgata ttgactgtac
     4741 ttcttttcgg aacagcatca gttgagcgac gataagcggc cggatacgct tcagttttct
     4801 gtcgatgcac ccgtaataaa gcctttgggc ggtgtgcccg gagtgtactt tttcgtcggc
     4861 acatcgggaa gaatgcgcac accggaagtg aatgtgtaag ggatctcctc tagcgggccg
     4921 gggaaagtca gctccagaaa acacgccttg tagcctttgg ccggtacatc aatggcaact
     4981 tcgtaaatgc cgtccgccgt cgcctcaatt tcccggggca cataggggca ggtgttgccg
     5041 cggaagtcac gagccttggg attccatgcc tcccacagcg tgacccggga gggctgcaca
     5101 atgtccccgt tggatgccgg aagcagctgt gctttcagcc ggttgtcgcc ttcaaaggag
     5161 aaactgtagt tggggatggg cagttcccgg gcattggcaa tgaaaaagga ggcaagactg
     5221 ttgtacgcct cagtacgatt cagaccgtgc cccacattag gcatatagcg gagccatttg
     5281 ggagcctgca gatcgttcca gtaaaactgt gaggagtcag gcagaaaaaa ctgatcgttg
     5341 cccgcattga ctacaagttt gggaatattc agtcgatcga cataactgta cgggtcgata
     5401 atggccatga gcgctttgtt ttccggggtc tccagccatt taaggatatc aagcgccagg
     5461 taatcgccca ctgccacaga ccacctgccg agcgcttcaa agtggtggcg aaaagaagcg
     5521 ggaacattga gcagatcaat ggaaaaaggc accaggcccc ggacacgggc atccacaata
     5581 gctgtagtcc aggttgtcca gccgcgcttg gaaccgccgg caacaataaa atccttcacc
     5641 tggttgccgc cgcccgcatc gctggcagcg aaagcctgca ccgcatccat ggcacgcacc
     5701 accgctttgg tcatgggaag acgcagcggc cattcctcat cgcctgtctg atagaatttg
     5761 agccaggtat aggcgataag cgcatcttcg cccctgccca ttcccatctg atcccagaaa
     5821 acgagaggct ggttagggat catatatacc gtggaggtta cacatcccga ctgttttgcc
     5881 aggtttttta tggtcgagtc agccgtgcgg ggttcttcac cctcccggtt gctgccgccg
     5941 tcaataaaca tgagtgctgt cgggtgagtc accgtatcgg gcacgcacac agtgatccaa
     6001 tgttcccaaa ggaggcggtt cacttcacct ttatgccgcc actgttggga gaccatgctg
     6061 agggtgtggc aggtgatccc gtcttctttt tccgtgagta cacgctccca tttgaagttg
     6121 ttgtcttcct tggcaacata gcggtccaga gccgtaagcc cttctttggc acgaccatgg
     6181 gcataactgc cgtctgcagg cgcatttccg gaaaagaata tcagggaaag aagcagcaca
     6241 aaagaggatg cggaaaaaag agatctcatg atttttctcc ttctgtgatt acaggaatac
     6301 cgcaagaaga caacatctcc agcgaacggc ccgccggtgt cattatagta cagacgtgcg
     6361 aaaagacata aaaagtttca cgcccgagga ccaacgcagg tgtgtaagct acggaaaaaa
     6421 tttatttcgc agtcagtccc gcgatttatc cggcaatatt tccgcacccc acgtcattcc
     6481 tgcctcccct gggaacgcca atcgacctat tggcatacaa gcgggtcatg atgaagacaa
     6541 gccacccgga ttcgccgcgg aaatatgtcc t
//
LOCUS       DAOPWB010000162         6503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_11800_length_6503_cov_4.242401, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000162 DAOPWB010000000
VERSION     DAOPWB010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11800_length_6503_cov_4.242401"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PKN92_11715"
     CDS             <1..1288
                     /locus_tag="PKN92_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49500.1"
                     /translation="LQPDHIRAQKHIKKIRILLKTTLRSPYLSDADDDGVYDSAAGLP
                     PEPPVVGNICPKCGAQNSEEFDRCWKCDASLALHEDSFLNLPSVQAHGPYLLRPETIS
                     SVAMFASFALFLVAIYFAVRLILTYKADSVEALTNVSQIYDQVLAPSRIMMSLVLLLW
                     WPYALKLSLRLARIKAMPPAILICISGLFLSSLTAVLVLMPHSMVFVAFLATLLISLL
                     LVLLAFQIPPGSALVAWAAHIVLVWLMAACSFWFAECYRFGEFLNPLQELPVLQETMS
                     GQGALQNAAPVRVPGFMTPIRQKVRWESSGSAWLDHYAHAARASVKKETAEQGLVFQI
                     LKGGNLIAHEPLEAEHLYVPFSIEPGEEYEFVVTGPDYAMVQIIVQSILPCVFVPSAP
                     PAVSADGAPAEAANEGTTATTEQGAGTLTAPPSVRP"
     gene            1425..3128
                     /locus_tag="PKN92_11720"
     CDS             1425..3128
                     /locus_tag="PKN92_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNZ49501.1"
                     /translation="MLGLGVWDWFALGIYFLVILLVGLWTSRKITNSEDFFIGGRRFG
                     KVMMIFFSFGAGTSGNDAVGVSAKSYTSGLSGIWYQWLWLFCTPFYWLIAPAFRRMRA
                     LTTGDFFEQRYNGSVASLYAFVGILVLTVNIGVIQLGAASMVEAATGGLIPRTAGILG
                     MAALYVAYGMAGGLAAAIITDFIQGILTLILSFMLLPPALKMVGGFAGLHASIGEESM
                     FSLVAPGEINLFFIVMLCLNALIGVVTQPHHMGVCAAGRTELEGQIGFAGGNLLKRLC
                     TIAWVLVGLCAVVLFKNSGREVEPDLVYGTVAQQLLPQIMPGLIGLFLAALMASMMSS
                     CDAFMVSCAGLFTQNIYKRWFRRNEKESHYVRVGRISSLVVVCCSLFFSFLVTDVPTG
                     LLWFFKLQALMGAAFWLGLFWRRATVAGAWAATCAGLFVLFGTSLSVFQKWAPAWLPD
                     YMFFDGKFRDSWQIAAYLSAAFFVGIVTSLCTRRVAQEKLDRFYNCLHTPVTPGEPPA
                     PEAFMLPEGVSAAVPVRLINHPDFEIPVPSRQALAGFLAIWVCVAALIGFVFWMARWG
                     A"
     gene            complement(3194..6055)
                     /locus_tag="PKN92_11725"
     CDS             complement(3194..6055)
                     /locus_tag="PKN92_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNZ49502.1"
                     /translation="MTSLMTRSLAIVAVIGVAFATALSAAAQGQTPTAPIRRPSPSSP
                     DASNWGTDASGKTQRIDRSKSGSSGSVSSRTEAGKETSTSSTGVGGRKGTSPSSSGAA
                     AGTASNAAAGDSAAAGTGAAERAASDKGAGREAPAARSAGAGVRSVSGGSGSRTTGGT
                     GADSGAGSTPFQKGGVSSVSSVNNGAFQPVLERELQYGPVPEDGEKITLEGPMPLEEF
                     LTAINLATNWNMVISPELKEISLRFWLYEVTPKQALEVLKFNKVYYEFDEEIGFLRVM
                     TKEEYLEREYGKAKPHEFKVVHADISYIESLVNSLLSPNGRVITDQRTNNIYVWDTQD
                     NIAEMERLVKEMDVPLKKAEYTIEHAELADIEAAVGNLMSQSGTLLTDVRTNQLFIWD
                     NPTVQAQIKETIARLDTPVESQTFFIIHVNAEDVLDSVESILSERGTIQMDPRFNTLV
                     VTDLPPRVAKIASLIQTLDQKLETRTWVLKYADPEFVADQIEAYIPGEMGQIVLQDEV
                     HQITATGLPSRLDEIDALVQVWDIRRKQVMIEAYIVEVSSEVERAFNVNWSYFANVGG
                     APFSLHSGSGGDPAAAATGDGQIASGGRKPYAVPRYGALEVDSSGKITRPLLKDIAGK
                     PVLDSYRGGNLAVTLDYLDSQHKATILSSPKVTVQDGEEALFENATKVPYVSASGGYG
                     YGYGYSGYPGGNTSDNLDYYRRMSSSGSRVEFIDVGTILSVLPRVTDDQNILLDISAE
                     DSTFTDKKIVVDDLSRTVPEKTLRRADTQVRVNSGDTIVLGGLRRDRTSNSSTRTPLL
                     GDLPLIGKLFRNPKKSSANSSLLIFMTTTIVGEDTMPEALSIATAESDIAGTSRKMKK
                     DIFGRMKDSISDGKNEIGVAVGQNGQIHCEGTIVTPDDLREKFFDAPKNMPVTVVIRK
                     HPAAPIEIVNEVTEAAMEAGLRIEFDNDLPPIVPSLKNQ"
     gene            complement(6248..>6503)
                     /locus_tag="PKN92_11730"
     CDS             complement(6248..>6503)
                     /locus_tag="PKN92_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49503.1"
                     /translation="PPPPPPPPPQAPDLAQMLADVKPTRQQVGAKVKFITPTNAKGVF
                     LGEGETINGVTIETISRTEVVFSFLWKEMNKTLTLTKARE"
BASE COUNT         1266 a         1696 c         1819 g         1722 t
ORIGIN      
        1 gctgcagccc gatcatatcc gggcgcagaa gcatatcaaa aaaatccgaa tcctcctcaa
       61 aacgacgcta cgaagcccat atctttctga tgcagatgac gatggagtct atgattccgc
      121 tgccggtctg ccacccgaac cgcctgtggt cggcaacatc tgcccgaaat gcggtgctca
      181 gaatagtgaa gagtttgacc gctgctggaa atgcgatgct tcactggcgc tgcacgagga
      241 ctcttttctc aatctgcctt ctgttcaggc gcacggtcct tatcttcttc gtcctgaaac
      301 gatcagcagt gttgccatgt ttgcttcctt tgcacttttt ctcgtagcga tttatttcgc
      361 cgtacgcctg atattgacct ataaagccga tagcgtggaa gcgctgacca atgtttcgca
      421 aatttacgat caggtcctgg cgcccagccg tattatgatg agccttgtgt tgctgctttg
      481 gtggccttat gcgctgaaat tgtcgctgcg tcttgcccgc atcaaggcta tgccgcctgc
      541 cattctgatt tgtataagcg ggctttttct gagcagtctc acggcggtgc tggttttaat
      601 gccgcattcc atggtctttg tggcttttct tgcgacgctg cttatttcgt tgctgcttgt
      661 cctccttgct ttccaaattc ctcccggcag tgcactggtc gcctgggcgg cacatattgt
      721 gctggtctgg cttatggctg cctgcagttt ctggtttgcc gaatgctacc gtttcggaga
      781 atttctgaat ccgctgcaag agctgccggt tctgcaggag accatgagcg ggcaaggagc
      841 gctgcagaat gccgccccgg tgcgtgtccc cggatttatg acgcccatcc gtcagaaagt
      901 gcgctgggaa tccagcggtt ccgcctggct cgatcattat gctcatgccg cccgggcaag
      961 tgttaaaaaa gagacggcag agcaggggct tgtctttcag attctgaagg gaggcaatct
     1021 gattgcccat gagccgctgg aggcggagca tctgtatgtg cctttttcca tagagcccgg
     1081 agaggagtat gaatttgttg tgaccgggcc ggactatgcc atggttcaga ttattgtgca
     1141 aagtatcctg ccctgtgtat tcgtgccgtc ggccccgcct gccgtttctg ccgatggtgc
     1201 accggcggaa gcagcgaatg aaggtactac cgctacgacg gaacagggtg ccgggacgct
     1261 gacagcgccg ccctccgtaa gaccgtaacc ccggggatca ggcagttttt ttgccgggta
     1321 tttcctcctg cacagaagga ggcagttgcg tcttcaggct gcatggcgcc ggaaagtacg
     1381 attttgcata tgccttggca cattatttag aagggaagaa gtctatgctg ggacttggag
     1441 tctgggattg gtttgcactg gggatttatt tcctggtaat tttgctggtg ggtctgtgga
     1501 cttccagaaa gataacgaac agcgaagact tttttattgg cggtcgccgc ttcggcaagg
     1561 tcatgatgat ctttttttca ttcggtgccg ggactagcgg caacgatgcg gtgggcgtgt
     1621 ccgccaaaag ttatacttcg ggtctttcag gcatctggta tcagtggctc tggcttttct
     1681 gtaccccctt ttactggctg atagcgcctg cttttcgccg gatgcgtgct ctgactacag
     1741 gtgatttttt cgagcagcgt tacaatggaa gcgtagcgtc actttatgct tttgtcggga
     1801 ttctggtgct gaccgtcaat atcggggtaa tccagctcgg ggcggcatcc atggtggagg
     1861 cggcgacagg ggggctcatt ccccgaacgg cgggtattct tggcatggct gctctttatg
     1921 ttgcctatgg tatggcgggt ggtcttgcgg cggcgattat tactgatttc attcagggaa
     1981 ttctcacctt gattctttct ttcatgctgc tgccgcctgc cctgaaaatg gtgggcggtt
     2041 tcgccggact gcacgcctcc attggtgaag aaagcatgtt cagcctggtg gcaccgggag
     2101 aaatcaatct ttttttcatt gttatgctct gcttgaatgc gctgatcggc gtggtgactc
     2161 agccccatca catgggggtc tgtgctgctg ggcgcacgga attggagggg cagattggct
     2221 ttgcaggggg caatctgctg aaacgtctgt gtacgatcgc ctgggtgctg gtcggtcttt
     2281 gtgctgtggt gctctttaaa aacagcggca gggaggtgga acctgatctg gtctatggta
     2341 cagtggctca acagctgctc cctcaaatca tgcccggact aatcggcctt tttcttgccg
     2401 ctcttatggc atcgatgatg tcgtcctgtg atgcctttat ggtttcctgc gcggggcttt
     2461 ttacgcaaaa tatctataaa cgctggttcc gccgcaatga aaaggaaagc cactatgtcc
     2521 gggtgggacg catcagctct cttgttgtag tctgttgttc cctctttttt tcctttcttg
     2581 tcaccgatgt gcccacagga ttactttggt tcttcaaatt gcaggcgctc atgggtgccg
     2641 cattctggct ggggctcttc tggcgccgtg ccaccgtcgc cggcgcctgg gcggcgacat
     2701 gtgcaggcct tttcgtattg tttggtacca gcctttccgt gtttcagaag tgggcgcccg
     2761 cctggcttcc ggattatatg ttcttcgacg gaaaattcag ggattcatgg cagatagccg
     2821 catatctctc cgccgccttt ttcgtcggaa tagtgacgag tctctgtacc cggcgggtgg
     2881 cacaggaaaa gctggatcgt ttttataatt gtctgcatac accagtcacg cctggcgaac
     2941 cgccagcgcc ggaagccttt atgcttccgg agggcgtaag cgctgccgtg cccgtccggc
     3001 tcattaatca tcctgatttt gagatacccg ttccgtcacg gcaggcgctt gcgggttttc
     3061 tggcgatctg ggtctgtgtt gccgctttaa taggctttgt gttctggatg gcgcgctggg
     3121 gtgcctgatc cggaggataa gaaaaaaggg caggaacgaa agctcctgcc cggaatgcag
     3181 cggtattgga gtatcactga ttcttcagtg acgggacaat cggcggcaga tcgttgtcaa
     3241 attcaatgcg gagccctgct tccatggcag cctctgtcac ttcgttgact atttcaatcg
     3301 gggcagcggg atgctttcga atcactacgg tgaccggcat attcttcggt gcatcaaaga
     3361 atttttcgcg gagatcatcg ggcgtgacaa tggtaccttc gcaatggatc tgtccattct
     3421 gaccgaccgc gacccctatc tcatttttcc cgtcggagat gctgtctttc attcgcccga
     3481 agatatcctt tttcatcttg cggcttgtgc cggcaatatc tgattccgcc gtggcgatgg
     3541 aaagagcctc cggcatggtg tcttcgccga caatggtcgt ggtcatgaag ataaggagcg
     3601 aggaattggc ggaggatttt ttcggattcc tgaagagttt gccaatgagc ggcagatcac
     3661 ccagcagcgg tgtgcgggtg gaagaattgc tcgtacgatc ccgacgcaag ccgcccagca
     3721 caatggtatc cccggaattg acccgcacct gcgtatcggc acggcgtaat gttttttccg
     3781 gcactgtacg ggacagatcg tcaacgacaa tctttttgtc ggtaaatgtg ctgtcttccg
     3841 cactgatatc cagcaggata ttctgatcgt ccgtgacacg gggcaacacc gaaagaatgg
     3901 tacccacatc aataaattcc accctgctcc cggaggaaga catgcgtcgg tagtagtcca
     3961 gattgtccga agtgtttcct ccgggatatc cgctgtatcc gtatccgtac ccgtatccgc
     4021 cggacgcact gacataaggt actttggtgg cgttttcaaa aagcgcctct tcaccatcct
     4081 gaacggtcac tttgggtgag gacagaatgg ttgccttatg ctggctgtca agatagtcca
     4141 gcgttaccgc cagattgccg ccgcgataag aatccagcac gggcttccct gcaatatcct
     4201 tgagcagcgg ccgggtaatt tttcctgaac tgtccacttc cagtgcgcca tagcggggca
     4261 cagcataagg cttgcgccct cctgaggcga tctggccgtc tccggttgcc gctgccgccg
     4321 gatcgccgcc tgagccactg tgcagggaga agggggcgcc gcctacattg gcaaagtacg
     4381 accagtttac attgaacgcc cgttccactt cggaactgac ttccacgata tacgcttcaa
     4441 tcatgacctg tttgcggcgg atatcccaaa cctgaaccag cgcatcgatt tcatcaagcc
     4501 ggctcggcag cccggttgcc gtgatctgat gcacttcatc ctgcaggaca atctgtccca
     4561 tttctccggg gatgtaggct tcgatctgat cggctacaaa ctcgggatcg gcatatttga
     4621 gaacccaggt gcgtgtttcc agcttctgat ccagtgtctg gatcagagag gcaattttgg
     4681 cgacccgggg aggcagatcg gtaacgacca gcgtattgaa acgcggatcc atctggatgg
     4741 tgcctctttc ggaaagtatg gattccacac tgtcgagcac gtcttctgca ttcacatgaa
     4801 tgatgaagaa agtctgagat tccacaggag tatccagccg ggcaatggtc tctttgatct
     4861 gtgcctgcac ggtggggttg tcccagataa agagttgatt tgtccgcaca tcggtgagaa
     4921 gtgtgccgct ctgcgacata agattgccca cagccgcttc aatgtctgca agttcggcgt
     4981 gctcaatcgt gtattccgct tttttcagcg gaacgtccat ttctttgacc agccgttcca
     5041 tttcggcgat attgtcctgc gtatcccaga cgtaaatgtt attggttcgc tggtctgtga
     5101 tcacacgccc gttcggtgaa agaagggaat tgacaagcga ttcaatataa ctgatatcgg
     5161 catgaactac tttaaactca tgaggcttgg ctttcccgta ttcccgttcg aggtattctt
     5221 ctttggtcat gacacgaagg aagccgattt cctcgtcaaa ttcatagtac actttgttga
     5281 atttgagcac ttcaagcgcc tgtttcgggg tcacttcgta cagccagaag cgcagagata
     5341 tttctttcag ctccggggaa ataaccatgt tccagttggt tgccagatta atggcggtta
     5401 aaaattcctc cagcggcata ggcccttcca gggtgatttt ctcgccgtct tcgggcacag
     5461 gaccatattg cagttcacgc tccaatacag gctgaaatgc gccattgttc accgaggaca
     5521 cagaactgac accgcctttt tggaatggcg tggagccggc accggagtcg gcacctgtgc
     5581 cgcctgttgt gcgtgacccg ctgcctcctg acacggagcg caccccggct ccggcggaac
     5641 gggcggccgg cgcctctctg cctgcgcctt tgtcactggc agcgcgctct gctgcaccgg
     5701 ttccggcggc agcactgtcg ccagctgcgg cattggaagc ggtgcctgct gctgctccgg
     5761 agctcgaggg cgaggtgcct tttcgtccgc cgacccctgt tgagctggta gaagtttctt
     5821 ttccggcctc tgttcttgag gaaacgcttc ctgaacttcc ggatttgctc ctgtcaatgc
     5881 gctgagtttt gcctgatgca tctgtccccc agttgcttgc gtcgggggat gaaggggaag
     5941 ggcgtcttat cggtgctgtc ggtgtttgcc cctgggccgc cgcggaaaga gcggtggcaa
     6001 aggcaacacc gataactgcc acaatggcaa gagaacgtgt catcaaagaa gtcatgacat
     6061 ccctttcaga taacagaaac tcatgtccgg cagaaaccgg agcgtctgtt ggcttttctc
     6121 ccgaaaaata aaaatccaca tacatcccac ccgctgcctt agcacgatga aaggcgcaga
     6181 aaggtcaccg atatgattgc atagtataca tgaaagacgt ggagaggcgc atggtcgcac
     6241 ccggacatta ttctctggcc ttggtcaggg taagggtttt gttcatttct ttccagagga
     6301 aagagaatac cacttcggtg cgggagatcg tttcgatggt gacaccgttg attgtttctc
     6361 cttcgcccag aaaaacaccc ttcgcatttg tgggtgtgat aaattttact ttagccccta
     6421 cctgctggcg ggtcggtttt acgtcagcca gcatttgtgc gagatccgga gcctgcggcg
     6481 gcgggggcgg cgggggcggg ggc
//
LOCUS       DAOPWB010000163         6466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_11892_length_6466_cov_5.330682, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000163 DAOPWB010000000
VERSION     DAOPWB010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11892_length_6466_cov_5.330682"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PKN92_11735"
     CDS             complement(<1..1068)
                     /locus_tag="PKN92_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNZ49504.1"
                     /translation="MKKSSPLLSSRRQWLRSTCAIALGAGYTAYAANKPGSYPFRATS
                     GDFIDEPQWDEKITVTVGPANADICGTTDKALQAAVDYALRLGGGTVRILPGTYRLRN
                     AVRLGSGIRLIGSGEDSKIVKENMLRTRLSEDSDWFDQEITLEDASGFQVGDAVCILG
                     KNPHTGGPLVVKRVLTARSGNRFKLDKGIRDNAWKMGEAEAATLFPLISVEDVENIVI
                     EDLCIDGNRENNDFFDGNYGGCIFMHDANNISVRRVIAQNNNGDGIAWQICHDVVVEE
                     CRSINHSGLGFHPGSGSKRTVMRNNFIQGNNIGIFFCWGVQNGLAENNICRENNTGIS
                     IGHRDNGNIVRNNEVYDSGTVG"
     gene            1243..1677
                     /locus_tag="PKN92_11740"
     CDS             1243..1677
                     /locus_tag="PKN92_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNZ49505.1"
                     /translation="MKLYTIGCGQRSAEDFFELLKKSGVRRIIDIRLHNTSQLAGFSK
                     AGDLPYFLKAIAGVDYVHEEAFSPSEEILDDYKKKKGSWADYEKAFKPLIKERHIEKK
                     ARELLQDGDCLLCMEHKPDQCHRRLVAEYLEKKIDGLEVEHL"
     gene            complement(1994..2069)
                     /locus_tag="PKN92_11745"
     tRNA            complement(1994..2069)
                     /locus_tag="PKN92_11745"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2034..2036),aa:Ala,seq:cgc)
     gene            2341..3078
                     /locus_tag="PKN92_11750"
     CDS             2341..3078
                     /locus_tag="PKN92_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ49506.1"
                     /translation="MSLLSIQNLSQSFDGKPILDGLTMDFWEGHIHAVVGPNGAGKST
                     LANTIMGLHGYTNYGGEIFFDGQSLRGVSVDARARMGITLAWQEPARFEGLSVLRFVG
                     AGSKRKDPAHVKGLLEALGLEGERYLHRSVDRTLSGGERKRIELASILAMEPRLILMD
                     EPDSGIDVAALDYIFTALSRLKNQGATVIMITHSRAVLEQADHAFLMCNGRLREKGEI
                     SKIGRYFIDRCIPCDHINEPDEEESIL"
     gene            3075..4034
                     /locus_tag="PKN92_11755"
     CDS             3075..4034
                     /locus_tag="PKN92_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNZ49507.1"
                     /translation="MSSSDLVAALLQSIGVDNPHGLSDNTARIEVHENRVVGLKLVPG
                     LKVDADETDDGINVEIRVEAGVRLQKPVHICFGVLPEDGRQHIDLDVRIHQDAHASFL
                     AHCTFPNAVDVRHTMTAGIEVEPGAHYAYFERHVHGTHGGITVIPEARVIVHDDAEFT
                     TEFELIKGRAGQIKFDYETLCHDRSVVEMTARISGRADDRIEIHERAQLLGDEAKAAL
                     ISHIALRDDARAEIYNDVTAYGARARGHVDCKEIVLDRAIAKAVPMVAVHNPTAHVTH
                     EAAIGSVDSRQLQTLLSRGLTEDEATDLIIEGLLSKKARWTKD"
     gene            4079..5053
                     /locus_tag="PKN92_11760"
     CDS             4079..5053
                     /locus_tag="PKN92_11760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNZ49508.1"
                     /translation="MKIAVTRRIPDAGLEVLKAWGGAKDIAVHETDEGIERGALLRLV
                     EGADAILSTVTEKMDAEAMHAAGPQLKIIANMAVGYDNISLADAAERKILVTNTPGVL
                     TEATADLAWTLILGASRRAGEAERCLRAGQWKGWGPRQFLGVDLHKKALGLFGLGRIG
                     RAVARRALGFEMSVLYHDVHRLSAEEEKALNARWAGKEELVSASDILSLHCPLTPETR
                     HAFTLNEFSKMKKTAVIVNSARGPVIREADLCQALDEGMIFAAGLDVFEEEPVVNPRL
                     FHHERAFLLPHIGSATMETRDTMARMAAQNITAALSGGTPANLIPLPL"
     gene            5160..5912
                     /locus_tag="PKN92_11765"
     CDS             5160..5912
                     /locus_tag="PKN92_11765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNZ49509.1"
                     /translation="MSRLANNWHNFVNRRKFAVIGKKCSLKGRFLEIDGHVEIGDFCR
                     IRNNVIMRTSGGGKIILGTYSGISYNCFLEARTVVKIGNYTGIAEFTVIRDTNHAVLG
                     TTDHWRLTPYISAPIVIGDACLIGSSCYIAPGVAIGDGAVIAQNSVVTKDVGPLEVWA
                     GNPARKVAHRVKGLPESMLARYEELLKRFGLRENRFGYHEEACRAGEAAMDGINRAAI
                     ERDRLAALFNPQEETKAEKDANDASASPEQEV"
     gene            6117..6383
                     /locus_tag="PKN92_11770"
     CDS             6117..6383
                     /locus_tag="PKN92_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49510.1"
                     /translation="MKKKLLLAYGCIALALCLRIVAPAEGEVVPTEGEISEGEEDTDI
                     IWGCGTKDPKVVTKDLLANWLLISLSLGCAPFRKCLAQKNVVCP"
BASE COUNT         1548 a         1570 c         1828 g         1520 t
ORIGIN      
        1 gcccaccgtg ccgctgtcat agacctcatt gttccggacg atgttaccgt tgtccctgtg
       61 gccgatggag atgcctgtat tgttttcccg gcagatattg ttttcggcga ggccgttctg
      121 cacgccccag cagaaaaaga tgccaatgtt attgccctgt atgaaattgt tacgcatgac
      181 ggtgcgcttg ctgcctgagc cggggtggaa tcccagcccg ctgtggttga tactgcggca
      241 ttcttccaca accacatcat gacaaatctg ccaggcaata ccgtcgccgt tgttattctg
      301 cgcgatcacc cgacgcacag aaatgttgtt ggcatcatgc atgaaaatgc agccgccgta
      361 gttgccgtcg aaaaagtcat tgttctctct gttgccgtct atgcacaaat cttcaatcac
      421 gatgttttcg acatcttcca cgctgatcag ggggaagagt gtggcggctt ccgcctcgcc
      481 cattttccag gcgttgtctc ggatgccttt gtccagtttg aagcggttac cgctgcgcgc
      541 tgtaaggaca cgcttcacaa caagcgggcc gccggtgtgc gggtttttgc cgaggatgca
      601 gacggcatca cccacctgaa aacctgatgc gtcttccagt gtgatctcct gatcgaacca
      661 gtccgagtct tccgacagcc gggtgcgcag catgttttct ttgacaatct tgctgtcttc
      721 gccgctgccg atgagccgga tgccactgcc cagacgcacg gcgttgcgta ggcgataggt
      781 gccgggaagg atgcgtacgg ttccgccacc cagtcgtaag gcatagtcca ccgctgcttg
      841 cagggctttg tcggtagtgc cgcagatgtc agcatttgcc gggcccaccg ttaccgtgat
      901 cttttcgtcc cactggggtt catcgatgaa gtcgcctgat gtggcgcgga atgggtaact
      961 gccgggcttg ttggcggcat aagcggtata accggcgccc agagcgattg cgcaagtgct
     1021 tcggagccac tgacgacgac tggaaagcaa aggggaagat tttttcacga cgtgtctttc
     1081 taatcaaaat aaaagttcag atagttgtgt tgtataaagc ctattattcc agaaatcgct
     1141 tgtgagaata cagctgtgac ataagcacag gaaaataact ttgcctggaa aaatgtttct
     1201 ttacaatata tagagaactt ttctcagaaa ggaaacagac tcatgaaact gtatacgatt
     1261 ggctgcggac agcgttccgc tgaggatttt tttgagctcc tgaaaaaatc aggcgtgcgc
     1321 cggatcatcg atatccgcct gcacaacacc tcgcaactgg cgggattctc caaggcaggc
     1381 gatcttccgt acttcctgaa agccattgcc ggtgttgatt atgtgcacga ggaagccttc
     1441 tcaccttcgg aggaaattct cgacgattac aaaaagaaaa aaggaagctg ggcggattat
     1501 gaaaaagcct tcaagcctct gattaaagaa cgccatatag aaaaaaaagc gcgggaactg
     1561 ttgcaggacg gcgattgcct cttatgcatg gaacacaagc ctgatcaatg tcatcgccgt
     1621 ctggtcgcgg agtatctcga gaagaaaatc gacggactcg aggtagaaca tttgtaaccg
     1681 gattgtcgat atgccgaaca gaccggggca taatgatttt ttaaccacag aggtacacag
     1741 gtctttcagc cacgaaggga tgaaggatac gaagccccac aaagagaata aaaaccaatg
     1801 attgggcacc gataaataca aacggcctgc gagcaacacc tgcgcagcgt cttccctgcg
     1861 aacgcaggaa acgggcaccg ctttggatac tattaaatcg aaagatttac gtgaattaat
     1921 gagctgtaca ggatcgcaat aaaaaactcc tccgatgcag tacaccggag gagttttgat
     1981 tttggaagaa aaatggtgga gctgaggggg atcgaacccc tggcctccgc attgcgaacg
     2041 cggcgctctc ccaactgagc tacagcccca ggaactgcat cagtatagca gattccgggc
     2101 cggtaatcca aaaaaacggc gcagacttgg cggtcgggtg cggggaaatc tcctgaaaat
     2161 caggagatgt cgtgcatgta tatggtatga taccggtttc agacccctct gactatggaa
     2221 gatctaaatt ctgggctgca gtgcggtttt tgtgcctgcg tgtgcagtat cgcttttctt
     2281 cccggcgagg catgtgttta tttcaggaaa cgagacctat tcgcatcagg agcaacagca
     2341 atgagcctgc tgagcataca gaacctgagc cagtctttcg atggcaagcc tattctggac
     2401 gggcttacca tggatttctg ggaggggcat attcatgcag tggtcggccc gaacggcgcc
     2461 ggaaagtcca cccttgccaa taccattatg ggactacacg gctataccaa ttacggcgga
     2521 gagatttttt ttgatggcca gtctttgcgt ggcgtgtcgg tggatgcccg tgcccggatg
     2581 gggatcacgc ttgcctggca ggaacccgcc cgctttgaag gcttgtcggt actgcgtttt
     2641 gtgggtgccg gaagcaagcg taaagatcct gcgcatgtca aagggcttct ggaagcactg
     2701 ggcctcgaag gcgagcgtta tcttcatcgc agtgtagacc gcaccttaag cggcggtgag
     2761 cgcaaacgca tcgagcttgc ttctattctt gccatggaac cgcgcctgat cctcatggac
     2821 gagcccgatt cggggatcga tgtggcagcc ctcgattaca tttttactgc cctgagccgc
     2881 ctgaaaaacc agggcgcaac cgttatcatg atcacacaca gccgtgcagt gctggaacag
     2941 gcggatcacg cttttctcat gtgcaacggc aggcttcgcg aaaaaggtga aatttctaaa
     3001 atcggacgtt atttcattga ccgctgcatt ccctgtgatc atattaatga gcctgatgaa
     3061 gaggagagta tcctgtgagt tcctctgatc ttgttgctgc cctgctgcag tctatcggtg
     3121 tggataaccc ccatggtctt tccgataaca cggcgcgtat agaagtgcac gaaaaccggg
     3181 tggttggttt gaagctggtt cccgggttaa aagtggatgc cgacgaaacc gatgacggca
     3241 tcaatgtgga gatccgggtt gaggcaggtg tgcggctgca gaagccggtt catatctgtt
     3301 tcggggtgct gcccgaggac ggccggcagc atattgatct ggatgtacgc attcatcagg
     3361 atgcccatgc gtcttttctc gcccactgca cctttcccaa tgcggtggac gtgcggcaca
     3421 ccatgactgc aggcattgaa gtggaacccg gtgctcatta tgcctatttc gagcggcatg
     3481 tgcacggcac gcacggcggt atcacggtga tccccgaagc acgtgtgatt gttcatgacg
     3541 atgctgaatt tacaactgaa ttcgagctga tcaaagggcg ggcaggtcaa atcaaatttg
     3601 attacgagac tttgtgccac gaccgcagtg tggtggaaat gacggcacgc atcagcggcc
     3661 gggcagatga ccgtatcgag atacatgaaa gggcacagct tctgggtgat gaagcgaagg
     3721 cagcgctgat ttcacatatt gccttgcgtg atgatgcccg ggcggagatt tacaacgacg
     3781 tgaccgccta tggtgcccgt gcccgcggac atgtggactg caaggaaatt gtactggatc
     3841 gcgccattgc gaaagccgtt cccatggtgg cggtgcacaa tccgactgcc catgttaccc
     3901 atgaggcggc gatcggcagt gtggattcac ggcagctgca gacgctcttg agtcgaggtc
     3961 tgacggaaga cgaagccaca gacctcatta ttgaagggtt attgagcaag aaagcacgct
     4021 ggacaaagga ttgaacagac ggcgcgacgt gctttggctt gtataaagga gagaatcatt
     4081 gaaaatagca gtgacaagaa gaattcctga cgccgggctg gaagtgctta aggcctgggg
     4141 cggtgcaaaa gatatagctg tccatgagac ggatgaaggt atagaacgcg gcgcgctgct
     4201 tcggctggtg gaaggggcag atgcgattct ttccacagta actgaaaaga tggatgctga
     4261 agcgatgcat gcggcaggac cgcagctcaa aattattgcc aatatggcag tgggttatga
     4321 caacatttct cttgccgatg cggcggagag aaagattctg gtaaccaata cccccggcgt
     4381 gcttaccgaa gccacagcgg atctggcgtg gacgctgatt ctgggtgcat cacggcgcgc
     4441 cggagaagcg gagcgctgtc ttcgcgccgg acagtggaaa gggtggggac cccgtcagtt
     4501 tctcggcgtg gatctgcata agaaagccct cggtctcttc gggctgggca ggatcggccg
     4561 tgctgttgca cgccgtgcgc tgggctttga gatgtccgtg ctttatcatg atgtgcatcg
     4621 gctcagcgca gaagaagaga aagcgttgaa tgcacggtgg gcgggcaaag aagagctggt
     4681 atcggcatcg gatattcttt cactgcactg cccccttact cctgaaacaa ggcacgcctt
     4741 cacgctgaat gagttcagca aaatgaagaa aacagcagtg atcgttaaca gtgcccgggg
     4801 acctgtaatc agggaagcag atctctgtca ggctctggat gaaggcatga tttttgccgc
     4861 cggtctggat gtctttgaag aagaacctgt ggtgaacccg cgcctgtttc atcatgaacg
     4921 ggcatttctg ttgcctcata tcggcagtgc cactatggaa acgcgggaca cgatggcgcg
     4981 tatggcggcg caaaatatta cagccgcatt gtcaggcggc acaccggcaa atctgattcc
     5041 ccttccccta tgagcgggaa tagatctcgt tcgggtgcat gcggtacagt aaaagaagca
     5101 cgtcgggctg atggagcccg gttttataat gagtactatg gaagaaggaa aaggcgttca
     5161 tgagcaggct ggcaaataac tggcacaatt ttgttaaccg cagaaaattt gctgttatcg
     5221 gcaagaaatg cagtctcaag gggcgttttc ttgaaattga cggacatgtg gaaattggtg
     5281 atttctgccg tatccggaac aatgtcatca tgcgcaccag cggcggcggc aagattatcc
     5341 tgggtaccta cagcgggatc agctataact gctttcttga agcgcggacg gtcgtaaaaa
     5401 ttggtaatta tactggtatc gctgaattta cagtcatacg cgacacgaac catgccgttc
     5461 tgggcaccac cgatcactgg cgcctcacac cctatatctc cgcacctatt gtgattggcg
     5521 atgcctgcct catcggcagc agttgttata ttgcgccggg agtcgccatc ggcgatggtg
     5581 ctgttattgc gcagaacagt gttgtgacga aagacgtggg tcctcttgag gtatgggcgg
     5641 gcaatcctgc acggaaggtg gcacaccgtg taaaggggct gcccgaatca atgcttgccc
     5701 gttatgaaga gctcttgaag cgttttggtc tgcgtgaaaa ccgttttggc tatcatgaag
     5761 aagcctgcag ggcgggggag gctgccatgg atggaatcaa ccgtgccgcc atcgaaaggg
     5821 atcggctcgc tgcgctattc aatccgcagg aagaaacgaa agcagagaag gacgctaacg
     5881 atgcttctgc ctcgcccgag caggaggtgt aggagggcta tattgaaaaa aataaacaaa
     5941 aaagaggcag gcttttgggc ctgcctcttt ctgatactcc acgtgttttc tctgcaataa
     6001 ttgcgcttct tgacataacc ttgccggagt ggcaaactca tttataatag tattgtcctg
     6061 gttatgatgt gcaacgcaac aatttgggtg gttaccaatc gaaaagggat cgatcaatga
     6121 aaaagaaatt gttgcttgcc tatggatgta ttgctctggc tttatgcctt cggatcgtcg
     6181 cgcctgcgga aggcgaagta gtacctacgg aaggtgagat ttctgaagga gaagaagaca
     6241 cagatatcat ctggggctgt ggtactaaag atccaaaggt agtaaccaag gatctgcttg
     6301 ccaactggtt gctgatttct ttatctctgg ggtgcgctcc tttccggaag tgtctggcgc
     6361 agaaaaatgt agtgtgcccc tgacgctatt ccaactttct cccgtcattc ctgctttctc
     6421 ccgtcatacc ttcctccccc cgcctcgtca ttcctgcgca ggcagg
//
LOCUS       DAOPWB010000164         6234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_12493_length_6234_cov_4.023790, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000164 DAOPWB010000000
VERSION     DAOPWB010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12493_length_6234_cov_4.023790"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKN92_11775"
     CDS             <1..352
                     /locus_tag="PKN92_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49511.1"
                     /translation="TAQIEQKSGRYLLQVPQWQGVVAEVRVNGQKSGVVYGQPPICDI
                     TDQITAGSNTVEFIVYGSQRNPLGPHLGNQDLGFCHPGSWNKAPVAPQPAGEDYFTMP
                     YGLLAPFELLHETF"
     gene            507..893
                     /locus_tag="PKN92_11780"
     CDS             507..893
                     /locus_tag="PKN92_11780"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNZ49512.1"
                     /translation="MKANSGFTLIELILVTVIIGILAGMVVANYSGRIRETQIRAAKG
                     DISTLSTQVDLYALDNNDSYPKTLEDLITGKRRYVRELQPDPWGNPYYYAPPTDILKA
                     DYKIYSAGPDGVPGNEDDVTSTSPMP"
     gene            927..1457
                     /locus_tag="PKN92_11785"
     CDS             927..1457
                     /locus_tag="PKN92_11785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ49513.1"
                     /translation="MIKGSLKRTACCRGFTLLELLVVVALIGTISLSVMPVFISAMNG
                     IQLRNARNDLIAAIRFAQEMAVRESVEYRIYFNTEEHSYRIMRLSGLNEKDEKQFEIA
                     ETLLGEKQVLPQYLEIERIKARKAPSSQEKYIACLPNGSSDVSEILLRDTRVRGNHFK
                     ISVEGPLGQVEIKDLR"
     gene            1454..1915
                     /locus_tag="PKN92_11790"
     CDS             1454..1915
                     /locus_tag="PKN92_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49514.1"
                     /translation="MKKCSRFGYIFVETVVAMGLLSLSAFVIQNALRQAMITRAQAQD
                     ITSARFLLEKISGERILLFQQPEGNGSGQCDPPFEKFRYEWSLERVDIPKPELPPGLS
                     PEERDVLEDAYIDFMGKLTVRILWQRGGADFEAVGESLVGSSILWTPEDTQ"
     gene            1912..2655
                     /locus_tag="PKN92_11795"
     CDS             1912..2655
                     /locus_tag="PKN92_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ49515.1"
                     /translation="MKRTGKETHATDVRGFTLMELLVATVLLSLVMSSVYSLTHASLR
                     TWRSVEGGVDMHLGARSFFTLFSHEYNSIVGRAEHLFEGDNKSITMFVVAQPMDLDEG
                     EGSRLMRVTYSYNRSKKTIEREEALVTGALPTRPPQDEQLDRSRIKLQRRYKSTVAEN
                     VGQFEITYIWVPLSDQITPGLPPEPEPPIYKKESRERWGLPQGIRLEVEMRDPDNKEK
                     TYQFNTVLPMRAQSARQKRDRLEEMMSAK"
     gene            2645..3595
                     /locus_tag="PKN92_11800"
     CDS             2645..3595
                     /locus_tag="PKN92_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNZ49516.1"
                     /translation="MPNNGMHKTGFVLISVLWVTAMLTVIALGFGRRAALERRASAYA
                     LDQAEAMLMARGAVERGIIELYNQRYMRMLLPEGQRGGDHCGQSWAKKKNLYTEGYFE
                     KRENFDNDEAAYIIIDEDRYINVNAADEKILEEVKSLKRPVIRAINARLNRAGGRGNE
                     EYQPFNAVEEIRYLRGVTEDNWFGSKNAPGLKNLLTVWGTAQINVNTASEEVLSCIPG
                     LKKTEINAILRYRAGSDGVLYTDDDQGFEDMNDLLTKAGLSESGSADWGAYCKFNASH
                     FRITGVATRRQGKVRAICSAIFAAGNYIQVLDWQEKTLGS"
     gene            3585..5075
                     /locus_tag="PKN92_11805"
     CDS             3585..5075
                     /locus_tag="PKN92_11805"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HNZ49517.1"
                     /translation="MARKIPQISVIQVAARGITATRFSMALKKPEQVTVRRIAGSWEG
                     EAYEEAFRTFINELKIQEDRVVLVLPRQDVTTRLLTLPSHSLEEVAGMVQFSAEEYVP
                     YTLDELVIDQCIIKTLDSGESEVMAAFPHKDVVNRPLSLLQQCGITPEKILLSTACLA
                     LTAVVDPLLSKERHALVSLMDGGMEILVLQDGIPVFSRGIISTQDWEVTAKDPDAGSG
                     AGLIVDSGAEELAGEIRNSLAAYRRESPDGLGVEHIHITCAYADVALLCRSLSESMNR
                     PCTPAVFTPPVLESVPCSGGAFPAVAVGALLSLTGRPEFDINLLPESESRARRIETIK
                     KRLLQGTAFAVVLLIALGGWYYQARSQRLALIRELEQRIARIEPNAKGITEKREALNI
                     LSRQVDRKGSILEQLAALVDAAPDHRLNFLRITLRKKEGVELWGRAKAVNDISDFAQN
                     IRSKAEGHLRFFAQARSLYEQQTQERDQTVFSYQIEALIQEEDNAE"
     gene            5065..6171
                     /locus_tag="PKN92_11810"
     CDS             5065..6171
                     /locus_tag="PKN92_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49518.1"
                     /translation="MLNKIIIRLLDLWAKLSLREKRLAMLTGSVVVLMLAVMTFQRVS
                     GSLRELDNTLSRLEDTLVSYTYQIAHREMVEAKYASVAAQHSSAWTEAEIHDRLRQEI
                     YRLARNSPQDLDKNGIPLNTPNTEGNLVEIPALGKGSMAEGGQGYREYMLNLRIPSST
                     LDNVIFFLERLQRSPQSLRIDGLEINRSPESELVSATIDISRIIADGTPGSGQDAPAA
                     PAEDISEEAVSTSASPKTGSWQGTAIQAEKLTLPGLPAREDNAVIASLQEQSEAYMLR
                     TLEKGAVYHMSLDLCAGSHDMTVGVGLEQDRAPFPGSQTITGNGESYRLQVQFTLPGK
                     PGGLVPVKCPWILFNEKDTKAEISNIVIRKIAEV"
BASE COUNT         1526 a         1640 c         1712 g         1356 t
ORIGIN      
        1 cacagcacag atagaacaga aaagcggtcg ttaccttctg caggttcctc agtggcaggg
       61 cgtcgtggct gaagtccgtg tcaacggcca gaaaagcggt gttgtctacg gtcagccgcc
      121 gatctgcgac attaccgacc aaataacagc cggaagcaat acggtggaat tcattgtgta
      181 cgggtcacag cgcaaccccc tcgggccgca tctgggcaat caggatctgg gcttctgcca
      241 cccaggctcc tggaataagg ctccggtggc accgcagccc gccggagaag actatttcac
      301 gatgccttac ggtcttcttg cgccctttga actgctgcac gaaactttct gaccgctgct
      361 aaccccataa ttcggataat tcatagtata tcagcaaaaa aaacgcctgt actgtcgcgt
      421 tgtcatacaa atgcagttgc gacatcgtta tagctgatgg tactattaag gcaaatgctc
      481 tgtacaggta atggagaagt gtttttatga aggcaaacag cggttttaca ctgatagaac
      541 tgattcttgt aacggtgatc atcggtattc ttgccggaat ggtggtggcg aactacagcg
      601 gtcgtatccg ggaaacccag atccgcgccg ccaaagggga tatttccaca ctcagcaccc
      661 aggtggacct ttacgcgctg gacaataacg acagttatcc gaaaactctg gaagacctga
      721 ttacgggcaa acgtcgttat gtccgggaac tgcagcctga tccctggggg aacccctact
      781 attatgcacc gcccaccgat attttaaagg cagactacaa gatctactca gccggtcccg
      841 atggcgtccc cggcaacgaa gatgatgtca cttccacctc accaatgccc taacgtggat
      901 gctgttcagg gcgggaaagt cccgttttga tcaaaggttc tttgaaaagg acggcatgct
      961 gccgcggttt tacccttctg gagctgcttg tcgttgtcgc tctgatcggc accatttccc
     1021 tttcggtcat gccagtcttt atcagcgcca tgaacggtat ccagctgcgc aacgcccgca
     1081 atgatctcat tgccgccatt cgctttgctc aggaaatggc ggtgcgcgag tcggtggaat
     1141 accgcattta ttttaataca gaggaacaca gttatcgcat tatgcggctc tccgggctga
     1201 acgaaaagga tgagaaacag tttgagattg ccgagacgct gctcggagaa aagcaggtac
     1261 tccctcaata tcttgagata gagcggatta aagcacgcaa ggccccttct tcccaggaga
     1321 aatacattgc ctgtctgccc aacggctcca gtgatgtatc cgaaattctt ttgcgcgaca
     1381 cccgagtccg gggaaaccat tttaaaatct ctgtagaagg gcctctgggc caggtggaaa
     1441 taaaggatct ccggtgaaaa agtgcagccg tttcggctac atatttgtgg aaactgttgt
     1501 cgccatgggg ctgctgagcc tgagtgcttt tgttatccaa aatgcactgc gtcaggcaat
     1561 gatcacccgt gcgcaggcac aggacataac cagcgcgcgt tttcttctgg aaaaaatcag
     1621 tggcgagcgt attctgctct tccagcagcc ggagggcaac ggttccggac agtgtgaccc
     1681 tccttttgaa aaatttcgct atgagtggag cctggagcgg gtggatatcc cgaaacctga
     1741 actgccgccg ggtctctctc ctgaagagcg ggacgtgctg gaggatgcct atatcgattt
     1801 tatgggcaaa ctgactgttc gtattttgtg gcagcggggc ggagcggact tcgaagctgt
     1861 gggcgaatca ctggtgggca gcagcatcct ctggacaccg gaggataccc aatgaagcgc
     1921 actggaaaag agacacatgc cacggacgtc cgggggttta ccctcatgga actgctggtg
     1981 gcgacggtgc ttctcagtct ggttatgagt tcggtctaca gtctgacgca cgcatctctg
     2041 cgcacctggc gcagcgtgga aggaggcgtg gacatgcatc ttggcgcccg cagttttttt
     2101 acgcttttca gccatgaata caacagcatc gtgggccggg cggaacacct ctttgaagga
     2161 gacaacaaat ccattaccat gtttgttgtt gcccagccta tggatctgga tgaaggggaa
     2221 ggctcccggc tcatgcgggt cacttattca tacaaccgca gcaaaaagac tattgagcgg
     2281 gaagaggcgc tcgtgacagg cgcacttccg acaagaccgc ctcaggacga gcaattggac
     2341 agatccagaa tcaagctgca gcgtcgctac aaatctacag tggctgaaaa tgtcggacag
     2401 ttcgaaataa cctatatctg ggtgccttta tccgatcaaa tcacccccgg tctgcctccc
     2461 gaaccggaac cgccgattta caagaaagaa tcaagagagc ggtgggggct gcctcagggc
     2521 atcaggctgg aagtggaaat gcgggatccg gacaacaagg agaaaacgta tcaattcaac
     2581 acagtactgc ccatgcgtgc tcagagtgcc cgccagaaac gggatcgcct ggaggaaatg
     2641 atgagtgcca aataacggga tgcataaaac aggattcgtg ctgataagcg tgttgtgggt
     2701 cacggcaatg ctgaccgtga tcgcactggg cttcggcaga cgtgccgctc tggaacgacg
     2761 tgcttccgcc tatgcgctgg atcaggcaga agccatgctc atggcacggg gcgctgtgga
     2821 acgtggcatt attgaattgt acaaccagcg gtacatgaga atgctgcttc cggaaggtca
     2881 gcggggcggt gaccattgcg gtcagtcctg ggcaaagaaa aagaatcttt atacagaagg
     2941 ttatttcgag aaacgggaga attttgataa cgacgaagcc gcctatatca ttatcgatga
     3001 agaccgctac attaatgtca atgcggccga tgaaaagatt ctggaggaag tgaaatctct
     3061 gaagcggccg gtaattcgtg ccattaatgc ccggctcaac cgtgcgggcg gccggggcaa
     3121 tgaagaatat cagcctttca atgccgtgga ggaaatccgc tatctgcggg gcgtgacgga
     3181 agacaactgg ttcggctcaa agaatgcgcc gggtctgaaa aacctgctca ctgtctgggg
     3241 aaccgcgcaa atcaacgtta ataccgcctc cgaagaggtg ctgtcctgca tacccgggct
     3301 gaaaaaaacc gaaattaatg ccattctgag gtatcgcgcc gggtcggatg gtgtattata
     3361 taccgacgat gatcagggtt ttgaggatat gaatgatctg ctgaccaaag caggtctgtc
     3421 agaatcgggc tccgctgact ggggcgccta ttgcaaattc aacgcttcac attttaggat
     3481 aacaggcgtg gcaacgcgtc gccaaggcaa ggtgcgcgct atttgctccg caatctttgc
     3541 ggctggcaat tatatacagg ttcttgactg gcaggagaaa acgcttggct cgtaaaattc
     3601 cacagatcag tgttattcag gtcgccgccc ggggaataac ggcgacacgc ttttccatgg
     3661 ccttaaaaaa gccggaacag gtgacagtcc ggcgtattgc cggcagctgg gaaggcgagg
     3721 cgtatgaaga ggcattccgc actttcatta atgagctgaa aatacaggaa gacagggtgg
     3781 tgctggtact gccccggcag gatgtaacga cccgcctgct gaccctgccc tcccacagcc
     3841 tggaagaagt ggcgggtatg gtgcaattca gcgctgaaga atatgtgcct tacacgctgg
     3901 atgaactggt cattgaccag tgcattatca aaacgctcga ttccggtgaa tcggaagtaa
     3961 tggcggcctt tcctcacaaa gatgttgtca atagaccgct atcccttctg caacaatgcg
     4021 gcattacacc ggagaagatc ctgctgagca cagcctgcct tgcgcttaca gctgttgtag
     4081 atcctctttt atcgaaggag cggcatgcgc tcgtttctct catggacggc ggcatggaaa
     4141 ttcttgttct gcaggacggc atccccgtat tcagccgcgg catcatttcc acgcaggact
     4201 gggaagtgac ggcgaaagat ccggacgcag gctcaggcgc cggtttgatc gttgactcag
     4261 gggcggaaga acttgccgga gaaattcgaa actctcttgc cgcctaccgg cgcgaatctc
     4321 ccgacggcct gggtgtggaa cacatccata taacctgtgc ctatgcggac gtagctttgc
     4381 tgtgccggtc gctgtcggaa agcatgaacc gtccttgcac acccgctgtc tttacaccgc
     4441 ctgtcctgga gagcgtgccc tgttcgggcg gggcattccc tgctgttgcc gtgggtgctc
     4501 tcctgagtct cacaggaaga ccggaattcg acatcaacct gctcccggaa tcagaatcca
     4561 gagcacgccg tatcgagacc attaaaaagc gccttctgca gggcaccgcc tttgcagttg
     4621 tcctgctcat cgccctcggc ggctggtatt atcaggcacg ttcacagcgg ctcgccctga
     4681 ttcgcgagct ggagcagcgt atcgcacgga tagaacccaa tgccaaaggg atcacagaaa
     4741 aacgggaagc cctcaatatt ctgagccgac aggtagaccg gaaaggttcc attctggagc
     4801 agcttgccgc tctggtggat gccgcaccgg atcaccggct taatttcctg cgtattacac
     4861 tgcgtaaaaa agaaggcgta gaactctggg gcagggcaaa agccgtcaac gatatctcgg
     4921 actttgctca gaatatccgc agcaaagcgg aagggcatct gcgttttttt gcgcaggcac
     4981 gcagccttta tgaacagcag acccaggaac gtgatcaaac agtgttttcc tatcaaatcg
     5041 aagcgctgat tcaggaggaa gacaatgctg aataaaatta ttattcgcct cctggatctc
     5101 tgggcaaaac tgtccctgcg cgaaaaacgc cttgccatgc tcaccggaag cgtggttgtg
     5161 ctgatgctgg cggtgatgac atttcagcgg gtttccgggt ccctgcgcga actggacaac
     5221 accctttccc ggcttgaaga tacgcttgtc tcctatacgt accagattgc ccatcgcgag
     5281 atggtggaag ccaaatatgc cagcgttgcc gctcagcact caagtgcctg gacggaagca
     5341 gaaatccacg acaggctccg tcaggaaatc tatcgtctgg cgcgcaattc cccccaggat
     5401 ctggacaaaa acggcatccc gctgaacacg cccaatacgg aaggcaatct ggtggaaatc
     5461 ccggcgctgg gcaaaggcag catggcggag ggcggccagg gctaccgcga atacatgctc
     5521 aacctgcgta ttccctccag cacgctggac aatgttattt tctttcttga acgcctccag
     5581 cgcagccccc aatccctgcg catagacggg ctcgaaataa accgttcgcc cgaaagcgaa
     5641 ctggtaagcg ccaccatcga catttcccga attattgccg atggaacccc cggatcagga
     5701 caggacgccc cggcagctcc tgctgaagat atctccgaag aggctgtatc gacttccgct
     5761 tccccaaaaa ccggaagctg gcagggcaca gcgattcaag ccgaaaaact gacgctgccc
     5821 ggtcttccgg cacgagaaga taatgctgtc atcgccagcc ttcaggaaca atcggaagcc
     5881 tatatgctgc gcacgctgga aaagggtgct gtctatcata tgagcctgga tttatgcgcc
     5941 gggtctcacg acatgaccgt aggcgtggga ctcgaacagg acagagcccc ctttcccggg
     6001 agtcagacga ttacgggtaa cggtgaaagt taccggctgc aggtacaatt cacgctgccc
     6061 ggaaagccgg gagggcttgt ccctgtcaag tgcccctgga tccttttcaa cgaaaaggac
     6121 accaaagctg aaatatctaa cattgttata cgaaaaattg cggaggtgta atggatcgtc
     6181 ttcttgccat aagtgccacg ggaatccttg cctgtattct gggcatgatc accc
//
LOCUS       DAOPWB010000165         6129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_12776_length_6129_cov_3.863681, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000165 DAOPWB010000000
VERSION     DAOPWB010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12776_length_6129_cov_3.863681"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..774)
                     /locus_tag="PKN92_11815"
     CDS             complement(19..774)
                     /locus_tag="PKN92_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ49519.1"
                     /translation="MTDTIKFLLYNIRYGTGTGLGYHLPLPFAGYFRPSVKNTSRIVD
                     FIKENQPDIIGLVEVDEGSYRSGKINQAQWIAEELGYHPVHETKYGTQSLARKMPILN
                     RQGNAFVTRRHVVAERFHFLETGMKRLVIELEFDNFVIFLVHLSLTFNSRQRQLTGLN
                     RIFQGITKPIIVAGDFNALTGSGELELFMNAANLINANSEELPTFPSIMPWRQLDFIL
                     HSPEIQMQNFAVCPVRYSDHMPVLCEFSCPSAA"
     gene            complement(954..2333)
                     /locus_tag="PKN92_11820"
     CDS             complement(954..2333)
                     /locus_tag="PKN92_11820"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNZ49520.1"
                     /translation="MRFKTLLIAALIVSLGGMAFAELQNVELGGSIRIRGNYFNMDSL
                     RDSSYVEQRTRLNVKADFTQDVSAFIEFDSYGFWGEGFRSNYLTGADARGNSDVALYQ
                     AYIEARNLWGTPLSLRVGRQEIALGSQMLVGTNDYSYNFSGLSFDALRLTFANDYVNI
                     DAIAAKLAETVSDFGDDDVDMYAVYGSYIGIEDVTLDAYWVFIRDDQQTDLRMRSILQ
                     DLDAYWVFIRDDQGALANILRNGTTVDIHTLGLRGAGVISGFDFDVEAAYQFGDVDGI
                     KQPWFRWKRSADVEYAEWAVNAEVGYTFDMTLQPRVFARFAYFGGGDPDTSCWSNDAD
                     RPFNRLFSNNQYSQFLDFNRNMSNFLAYTLGVSAMPTEAIELKLVGSFYESDQRAGNS
                     GRSLGWEVGLFADYNYSEDLVIRGGYSHFFGRKGLSGDVIVNNGLFGWPGEKKDDYDY
                     VFIETELAF"
     gene            complement(2660..3979)
                     /locus_tag="PKN92_11825"
     CDS             complement(2660..3979)
                     /locus_tag="PKN92_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49521.1"
                     /translation="MSLVELIVAIAIFTTIMSGVVLLFSTVTNTVRRSYQTMALFEQT
                     NNAVLAIERDVQTLFNNPNSGTEVRFYGEPFGFVIVGIDEYNKVSRITYAVHRDTARI
                     DEPQAVTGRGETITLPRRWDQLQALSTTDLSAFYYRAPGQDWVDVEVEVIYGVLLRFV
                     ETDVAGLQNFPKMNALLASGATPRLPRPSAFSDESGVGSPARFPWLTSYLWTGTVAAS
                     VPPHARDAFKTAETCHYWLQLLNGPGLPGAAAWDATDIWWREKQGALLLDQFWYDDHL
                     PVPPYPDTQRFHLQQHVVAENFLLNAFLLDPQTGVRIKNRDDTFVDILGPAQEPIFRY
                     AVEEYGQRNSYFNTLFNLTGKDTDGVGFMEKTMTTLDPRYLLALLNDRDFYELGNPAQ
                     GRVPVSLDVAMWSLNESASTGGAVVLNRFARTIYLPAGYMRPRQVKQ"
     gene            complement(4005..4409)
                     /locus_tag="PKN92_11830"
     CDS             complement(4005..4409)
                     /locus_tag="PKN92_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNZ49522.1"
                     /translation="MIGTPGSRQAGFTLLETLVALVILATGMVSVLLLFPITLDRQRV
                     NTQRSSTTAFVQSELAKIQAKGTGFGFHEWLEEHRFRALEDNLTANPFIKATVVSWQP
                     VGEIDMGLYRVTLGLRMHDGSVEYFTTYVTDR"
     gene            complement(4406..5452)
                     /locus_tag="PKN92_11835"
     CDS             complement(4406..5452)
                     /locus_tag="PKN92_11835"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNZ49523.1"
                     /translation="MVQRFLTYRHRTRSRSGWTLIELLVVLAIITILAAFSVPVVNMF
                     SRNDLRDSARMLYTLLRAARVYAVNNNVETAVVYFLDNPFSKEADGTVTFSPMEDSVS
                     DTWVRTIVAATVMYRLPAEMPVWPILPPDVQQAEDSDSITGSEEAVDWRKAGIFVPTP
                     GMGDIVSFPDGYSLLLNLPREDFEKMPGGSGTAQERALNALGSPVYQVDGALGYAPPE
                     KQNFLFHYNRQMERGAGTSPLLENLGMRPVYVYPETPSEGIYVDSVTSLAQVMPLFDP
                     DLVRPHIAHVFTPRGSLKTRVQGVKERYRLLVAPLPSAFPEDRLITYPDGEIDAFGAL
                     VEINRGTGYVRLEQ"
     gene            complement(5592..>6129)
                     /locus_tag="PKN92_11840"
     CDS             complement(5592..>6129)
                     /locus_tag="PKN92_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49524.1"
                     /translation="RLGRDTLSTGADSIAQLYVDRDVLAKLGTNYMDIGLDPWGQIYR
                     AWPGPWRFSTAGAPPGKRWPIPFRIFSIDMSNTDAASRFTVKPDGFEFSPQDAMLYAV
                     PDAATWPSKVNSPAQPNKPAYLWSYGGNSRSSQMLYQAVYSNDPNEWFYSESEEDIAG
                     GDDVNNWDNGSSWQRFYQ"
BASE COUNT         1553 a         1654 c         1602 g         1320 t
ORIGIN      
        1 caggacactt ttgtataatc atgcggcgga agggcaggaa aattcacaga gaaccggcat
       61 atgatccgaa tagcgaacag gacaaaccgc aaaattctgc atctgaattt caggactgtg
      121 aagaataaaa tccagttgcc tccaaggcat aatgctcgga aacgtaggca gttcctcgct
      181 gttggcgttg atcagattgg cggcattcat gaacagttcc agctcgccgg aaccggtaag
      241 cgcattgaaa tcaccggcaa caatgatggg ctttgtgatt ccctgaaaaa tccggttgag
      301 accggttaac tgtcgctgtc gcgaattgaa ggtcagagac aggtgcacca gaaagatcac
      361 aaagttatca aattccaatt caataaccag gcgcttcatc cctgtttcaa gaaaatgaaa
      421 gcgctctgcc acgacatgcc ttcttgtaac aaaagcgttg ccctgtctgt tcagaatagg
      481 cattttgcgg gcaagagact gcgttccgta cttcgtttca tgcacaggat gatagcccaa
      541 ttcttcggca atccattgtg cctgattaat ttttccggaa cggtaagacc cctcatccac
      601 ttccaccagt ccgataatat cgggctggtt ttctttgata aaatcgacga ttcgggaagt
      661 gtttttcacg ctggggcgaa aatatcctgc aaagggcagc ggcaaatgat agccgagtcc
      721 tgtgccggta ccgtaacgga tattatacag aagaaattta atcgtgtcgg tcacgttgca
      781 aaaaaacctc attgtgtgca ggaagcgcaa tcaagcggga ggactggcgc cgcttcgaat
      841 gtagatatac ggtcacccca aaacgtgaac agtatactac aaaagacaaa gcctccgcaa
      901 cagggggctg ctgcggaggc tgttaaccaa ccagataaga atgtcaggtc aaattagaag
      961 gcaagttcgg tttcgatgaa gacgtagtcg tagtcgtctt tcttctcgcc gggccagcca
     1021 aagagaccgt tgttgacgat gacatcgcca ctcaggcctt tgcggccgaa gaagtgggag
     1081 tatccgccac ggatgaccag gtcttcgctg tagttgtagt cagcaaagag accgacttcc
     1141 cagccaaggc ttctgccgct gttgccagcg cgctgatcgg attcgtagaa gcttccaacc
     1201 agtttcagtt caatggcttc ggtcggcatg gcactaacac ccagcgtgta ggcaaggaag
     1261 ttactcatgt tgcggttgaa gtccaggaac tggctgtact ggttgttgga gaacagacgg
     1321 ttgaagggac ggtcagcatc gttgctccag cagctggtgt cgggatcgcc gccgccaaag
     1381 taggcgaagc gggcgaagac gcggggctga agggtcatgt caaaggtgta gccgacttcg
     1441 gcattcactg cccactcggc gtattcaaca tcagcagaac gtttccagcg gaaccagggc
     1501 tgtttgatac catcaacatc accaaactgg taggctgctt caacgtcgaa atcaaatcca
     1561 ctaataacac cagctccgcg gaggccaagt gtgtgaatgt cgactgtggt gccgtttctc
     1621 aggatattgg ccagcgcacc ctgatcatca cggatgaaca cccagtaggc atccaggtcc
     1681 tgcaagatcg aacgcatacg caagtccgtc tgctgatcat cacggatgaa cacccagtag
     1741 gcatccaggg tcacatcttc aatgccgatg tagctgccat agacagcgta catatcaaca
     1801 tcatcatcac cgaaatcact gacggtttcg gccagtttgg cagcgatggc gtcaatgttg
     1861 acatagtcat tggcgaaggt cagacgaagg gcgtcgaagg acagaccgga gaaattatag
     1921 gagtagtcat tggtgccgac cagcatctgg ctgcccagag cgatttcctg gcgaccaacg
     1981 cgcaggctga ggggtgtgcc ccacaggttg cgggcttcaa tgtaggcctg atacagagcg
     2041 acgtcagagt tgccgcgggc atctgcaccg gtcaggtagt tggagcgaaa tccttcgccc
     2101 cagaagccgt aggagtcgaa ttcgatgaac gcggagacgt cctgggtgaa gtcagctttg
     2161 acgttcaggc gtgtgcgctg ttcgacatag ctgctgtctc tcagggaatc catgttgaag
     2221 tagttgccac ggatgcggat gctaccgccg agttcaacat tctgaagttc ggcaaaagcc
     2281 atgccgccca ggctcacgat aagggctgca atcaagaggg ttttaaaacg catatgcgca
     2341 ttctccataa ataaaattgg gttgactacc atgattcgct tcggaaaaca tttccgcgcg
     2401 gcatcttgta ctgaagatgc tatttttata tggcgaactt cacgaaaaac agcacgctgg
     2461 attggtgtaa tttaacgctt ccatccctcc tttcctttat gaattaggtg ctgttcgtga
     2521 ttcaaatatc cggtcaatac atacaactac atacgggact tttctgtccg tgaaccctat
     2581 tttaacacaa ccaattgtct ttgtcaagta ttatccccca aaattcaggc agggcagcac
     2641 atgcctgcga agcagcattt tactgcttaa cctgacgggg gcgcatatag ccagcaggca
     2701 gataaatggt tctcgcaaag cgattcagca ccacggcacc gcccgtgctc gctgattcat
     2761 tgaggctcca catggcaaca tccagggaaa cgggcacgcg cccctgagcg ggattaccca
     2821 gttcgtaaaa atcacgatca ttcagcaaag caagcaaata gcgcggatcc agcgttgtca
     2881 tggttttttc cataaatccg acgccgtccg tgtctttgcc cgtgaggttg aagagcgtgt
     2941 tgaaatagga attgcgctgt ccgtactctt cgacggcata acggaaaatg ggctcctgcg
     3001 ccggtcccag gatatcaaca aaggtatcgt cgcgattttt gatacgaacc cctgtctgcg
     3061 ggtcgagcag gaaagcattc agtagaaaat tttccgcaac aacgtgctgc tgcagatgaa
     3121 aacgctgcgt atcgggataa ggcggcaccg gaagatgatc atcataccag aactgatcca
     3181 gcagaagcgc tccctgtttt tcccgccacc agatgtctgt tgcatcccag gcagccgcac
     3241 cgggaaggcc gggaccgttc agcagctgaa gccagtaatg gcacgtttcc gctgttttaa
     3301 aagcgtctct ggcgtgaggc ggcactgatg ccgcaacagt gcccgtccac aaataactgg
     3361 tgagccaggg aaaccgcgca ggcgatccga cgccgctttc atcggagaaa gctgaagggc
     3421 ggggaaggcg gggcgtggca cccgaagcca gaagcgcatt catcttggga aaattctgca
     3481 ggcctgccac atccgtctcc acaaaacgaa gcagcacccc gtagatcact tccacttcca
     3541 cgtccaccca gtcctgaccg ggcgcacgat aatagaaagc actgagatcg gttgtgctca
     3601 gagcctgtaa ctgatcccac cggcgcggca gggtaatggt ttctccgcga ccggtgacgg
     3661 cctggggctc atcaatccgt gctgtatcac gatgcaccgc ataggtgatg cggctgacct
     3721 tgttgtactc atcgatgccc acaatcacaa agccgaaggg ctctccgtaa aagcggactt
     3781 ccgtgccgga attgggattg ttgaaaagcg tttggacgtc acgctcgatg gcaaggacgg
     3841 cattattggt ctgctcgaaa agtgccatgg tctgataact gcgacggacc gtatttgtga
     3901 ccgtgctgaa cagaagcacc acaccgctca taatggtcgt gaaaatggcg atcgccacaa
     3961 tcagttccac gagcgacata ccgcgcctcc cggcgctatg ggtatcagcg gtccgtaaca
     4021 taagtcgtaa aatactccac agatccatca tgcatgcgca agccgagagt tacccggtat
     4081 aaccccatat cgatctcacc cacgggctgc cagctgacaa cggttgcttt gataaagggg
     4141 ttggcggtga gattatcttc aagcgcccgg aatcggtgct cttccaacca ttcatgaaaa
     4201 ccgaatcccg tcccttttgc ctgaatcttc gccaactccg attgaacaaa ggctgttgtg
     4261 gaactgcgct gtgtgttgac tcgctgccgg tcaagggtga tcggaaaaag aagcagtacc
     4321 gataccatgc ctgttgcgag aataaccagc gcaacaagcg tctcaagcag cgtaaatcca
     4381 gcctgacggc tgcccggtgt tccaatcatt gctctagcct cacgtaaccg gtaccccggt
     4441 tgatctccac cagtgccccg aaggcatcta tctcgccgtc aggataggtg atcagcctgt
     4501 cttccgggaa agcagaaggc agcggtgcca ccagaagccg atatctttcc ttcacaccct
     4561 gcacccgggt cttcagggag ccgcgcggtg taaacacgtg agcgatatgc ggccgaacca
     4621 gatccggatc gaaaaggggc ataacctgcg ccagggaggt taccgaatca acatagatgc
     4681 cttctgaagg tgtctccgga tacacataaa cagggcgcat ccccagattt tccagaagag
     4741 gcgatgtgcc cgccccccgt tccatctgcc ggttgtaatg aaacaggaag ttctgttttt
     4801 cggggggcgc atagcccagc gcaccgtcaa cctgatacac cggagaaccg agcgcattca
     4861 gtgcacgctc ctgtgctgtg cccgatccgc ctggcatttt ttcaaaatct tcccggggca
     4921 gattcaaaag aagcgaatat ccgtccggaa aagaaacaat atcgcccatt cccggcgtag
     4981 gcacaaaaat tcccgctttg cgccagtcga ccgcttcttc actccccgta atggaatcgg
     5041 aatcttccgc ttgctgcaca tccggcggca gtatcggcca cacgggcatc tccgccggaa
     5101 gccgatacat gaccgtggca gctacaatgg tacgcaccca ggtatccgac acggaatctt
     5161 ccatcggaga aaaagtaaca gtgccgtccg cttccttcga aaaaggatta tccagaaaat
     5221 agacgactgc tgtttccacg ttgttattca cggcatagac acgggcggca cgcaaaagtg
     5281 tgtacagcat tctggcactg tcccgcaagt catttcgtga aaacatattc acgacaggaa
     5341 cagaaaaagc ggcaagaata gtaatgatcg ccagcacgac cagcaattcg atgagcgtcc
     5401 agcccgagcg actgcgggta cgatgtctgt atgttaaaaa gcgctgcacc acgttgaaac
     5461 ctgcctgtat aagtgtccgg tctcgctatc cctgccgccc cacccggacg gacagatcgt
     5521 tgatcagcag aaatcaatag aaaactgcag gcggaaaagg aaaggaccct cccgcctgca
     5581 gcacaacact cttattgata gaaacgctgc cagctgcttc cgttgtccca gttgttgacg
     5641 tcatcaccgc ccgcaatatc ctcttccgat tcggaataga accactcatt gggatcattg
     5701 ctgtaaacag cctgatacag catctggctg ctgcgggagt ttccgccgta tgaccacaga
     5761 tatgccggct tgttcggctg cgccgggctg ttcactttgc tcggccaggt cgccgcatcg
     5821 ggcaccgcat agagcatggc atcctgcggg gagaattcaa agccgtccgg ttttacggta
     5881 aaccgggaag cagcgtctgt attgctcata tcgatgctga aaatgcggaa gggaataggc
     5941 caccgctttc cggggggagc tcctgctgtg gagaagcgcc acggccccgg ccacgcacgg
     6001 taaatctgac cccacggatc cagcccgata tccatatagt tggtgcccag tttggcaagc
     6061 acatcacgat caacatacaa ctgcgcaatg gaatcagcac ccgtcgacag cgtgtctctg
     6121 ccaagacgc
//
LOCUS       DAOPWB010000166         6086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_12894_length_6086_cov_5.740342, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000166 DAOPWB010000000
VERSION     DAOPWB010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12894_length_6086_cov_5.740342"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1563
                     /locus_tag="PKN92_11845"
     CDS             253..1563
                     /locus_tag="PKN92_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNZ49525.1"
                     /translation="MIRALSLLFPFVFLCSASMSMAEAIAGNPQAIADLQAGRRSDAN
                     AAWWGFDREDSTEMLQAALDSGADRVVIPYTGAPWITRPLVLRSHQTVFFEPGVVVEA
                     KKGAFLGKSDSLFRLEKGEDLTLLGYGATLRMHKEDYLTPGYDNAEWRMTLSFTSCRR
                     IHVEGVTLESSGGDGIYLGNSSGEQPWCEDVVIRNVRCMNHHRQGISVITAKNLLIEN
                     CLFADTDGTAPRAGIDFEPNQADERIENCVVKNCVMENNAGPGILVYLMPLSRESAYV
                     SLRFENCHVRSGRDAGISVGAIKDDGPGGLIEFVSCTVENTYGRGLHIYDKGADRTKV
                     RFTDCRWSNTALRGDKGQKNLAPLVFTLNKTDNVSRLGGVEFIRCRVFDPFDRPVLIA
                     DTKDTPLSDIEGTIQFFSPTENPVALPDAQDAVTLSLEKEISFP"
     gene            1844..3148
                     /locus_tag="PKN92_11850"
     CDS             1844..3148
                     /locus_tag="PKN92_11850"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNZ49526.1"
                     /translation="MSADFTREHWEWRIRVLVSTYLGYGGFYLTRKVFSICKTPIAEE
                     LGWELGRTAHIWTAFLVAYMLGQFISSYAGRRWGPRLLLLGGLGISLLCNLIFGFANS
                     YYTFLVFMAFNGFVQAAGWPGCVGAISEWLRVKERGRIFGIWTTNYIVGSVMVKTLTG
                     MFLGMTGLYLGLAGWRWAFFGCTALAFLLWWLLYFWQRTRPEDVGLTPLVTEEETERQ
                     TIHASKEDQVGMKDYLILALNPLVLTMGGAYFCVKFLRYALDSWLPSFLIIQGLDPSL
                     SSYYSMGFDIAGLAGSVATGYMLDKWFKGNWAALCLVLSLGVIGGYLAVMYLGASPIA
                     IALCFSIVGFMLYGPDILLSGAGPVEIAGARNGMVVAGIVNGLGSIGPIVQEEVIGRL
                     VRGDVQQGMANTNRLALGISVLLMLLFIPMVIRLQIARRKRS"
     gene            complement(3145..4011)
                     /locus_tag="PKN92_11855"
     CDS             complement(3145..4011)
                     /locus_tag="PKN92_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ49527.1"
                     /translation="MRTLTPKQHIVRTRNADFSVWEYPGDGTPLFFCHCTGVSGRVWE
                     SVFRTLSLNHTLFAWDARGHGDSAKSPEAEAYTWDSLARDLLGVFDQMGLPEGMQAVG
                     HSGGGSTIVQAELIRPGTFSRLMLVDAIIALPDFFAGAKLLAKLVRRRRSRFSSRQEA
                     RERFSAKEPMNMWTGDSLDCYLKYCFFQGDGEEILLKCPGDIEAWVYEMSDKTEIMSR
                     LPQLQMPVLLITGENSYMQQYVEEQHKRIAGSEMQVIPGAGHFLAQEKPEEIAAVMNR
                     WFAPSEGIAQAH"
     gene            complement(4030..5124)
                     /gene="thrC"
                     /locus_tag="PKN92_11860"
     CDS             complement(4030..5124)
                     /gene="thrC"
                     /locus_tag="PKN92_11860"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNZ49528.1"
                     /translation="MYNPGLIARFRSHLPVSDTTRIITLNEGSTPLVAAPGLSAAIHP
                     RLEIYLKYEGLNPTGSFKDRGMTMAITKAVEEGFEMVMCASTGNTSASAAAYAARAGI
                     KCVVLIPEGKIAFGKLSQAMIHGAQVIQIRGNFDEALNLVRRMCNNFPIALVNSVNPY
                     RIEGQKTGAFEILEDFDGIAPDFQAMPVGNAGNITAYWKGYKEYHEMGKCEGRPRMLG
                     FQAAGSAPIVLGHPVLQPETFATAIRIGNPASWTTAEAARDESGGVIDMVTDDEIRDA
                     YTLVARTEGIFCEPASAASIAGLVKLSKAGYFDECTPRTGNMLRAVCILTGHGLKDPD
                     NAIAAASQPRTVEAVEEQIVEVLGFAPAMA"
     gene            complement(5145..>6086)
                     /locus_tag="PKN92_11865"
     CDS             complement(5145..>6086)
                     /locus_tag="PKN92_11865"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNZ49529.1"
                     /translation="PELVEAAVQHGVELRFEAAVAGAVPIIKALRESLAANRIEGIYG
                     ILNGTCNYILSRMTYEGLEFEETLNQAIANGFAETPPDLDILGHDTAHKCQILASLAY
                     STRIDLNAIPVEGITSITHLDVAYAREMGYIIKLLAIIREVDGSIEARVHPALIPESH
                     LLAAVRNEFNGIYVKSDCADATLYYGRGAGRKPTASAVVGDIVDIARNKGCAPFKPFT
                     YSRNLAIRDIGLAEGCYYLRLTTEDHPGVLGRVCSTLGKHGVSIASCIQKNQGDQEPV
                     HVVLMTHATVESAIKAALSEIDGFDCIAEPTQFLRVF"
BASE COUNT         1261 a         1689 c         1706 g         1430 t
ORIGIN      
        1 gagatatgtc gcagggcatc cctctgtcac cccggctcct ctccacgtca tccctccttc
       61 cccccgtcgt cattccagct cccccccacg tcatccctgc ttcccccccc acgtcattcc
      121 tgcgcaggca ggaatcttac cgcagaggcc tgctttttgt ccgtgcgccg gtgctgcttt
      181 tcctttactc gcgacgggcg aatcttttac aatgcaaaaa aacgtgtctc cattcagcag
      241 gaaaggcata ccatgatccg tgcattgtcc cttctttttc cctttgtgtt tctctgctcc
      301 gcctctatgt caatggcaga agcaattgcc ggaaatccgc aggccattgc tgatctgcag
      361 gcaggcaggc gcagcgatgc caatgcggcg tggtggggat ttgaccggga ggacagcacg
      421 gaaatgctgc aggcggcgct ggactcaggg gcagaccgcg tggtgattcc ctatacgggt
      481 gcaccgtgga ttacccgacc gctggtgctt cgcagccatc agacggtttt tttcgagcct
      541 ggtgtggtgg tggaggcaaa aaaaggcgcc ttcctcggga agagtgattc acttttccgc
      601 ctggaaaaag gagaggacct gacactcctg ggctacgggg cgaccttgcg tatgcacaag
      661 gaggattatc tgacgccggg ctatgacaat gccgagtggc ggatgaccct ttcttttacc
      721 agctgccgcc ggattcacgt ggaaggggtg acgctggaaa gcagcggcgg tgacgggatt
      781 tatctgggga acagcagcgg agaacagccc tggtgcgagg atgtggtgat ccgcaatgtg
      841 cgctgcatga atcaccaccg gcaggggatc agcgtgatca cggcgaaaaa tctgctgatc
      901 gaaaattgcc tctttgccga tacggacggc acggcgcccc gggcaggcat cgactttgaa
      961 cccaaccagg ccgatgagcg tatcgaaaac tgtgtggtga aaaattgtgt catggaaaac
     1021 aatgcgggac cggggattct ggtatatctg atgccgttga gccgtgaaag tgcctatgtc
     1081 tctctgcgct tcgaaaactg tcatgtacgc agcggaagag atgcgggcat cagtgtgggg
     1141 gcaatcaaag acgacggccc gggtgggctg atcgagtttg tctcctgtac ggtggagaat
     1201 acgtacgggc gcgggctgca catttacgac aaaggagccg accggacgaa agtccgcttt
     1261 accgactgcc gctggagcaa tactgccctc cgcggcgaca aaggtcagaa aaatctggcg
     1321 ccgctggttt ttacgctgaa caaaacagac aacgtgtccc gcctcggcgg tgttgaattt
     1381 atcaggtgcc gcgttttcga tcccttcgac cgcccggtcc tcattgccga cacgaaagac
     1441 acacccctca gtgacataga gggcacgatc caattcttca gcccgacaga aaaccctgtc
     1501 gccctcccgg atgcacagga cgccgtgact cttagtctgg aaaaagaaat ctcattcccc
     1561 tgagcgccat agccgtccat ttaatcgccc aatgccactc gcctgctcca attgcattac
     1621 aaggattcgg aagatttctg cttcaggcac tcaggcttta agaaccgtcg gcggacaata
     1681 acgcaccctg aatacatgta aaagttattg tcataaacaa gaaatactaa accccggagt
     1741 tcatcctccc cttttccctt ttgccgggtt ggactgaaag cgtctgtttc ctatagtatt
     1801 tacggaacga gaatgaaaaa tctgacgccg gagaggaaaa attatgtcgg cagactttac
     1861 ccgggagcac tgggagtggc ggatacgcgt tctggtttcg acttacctgg gctatggtgg
     1921 tttttatctg acccgcaaag tcttttccat ctgcaagaca ccgattgcgg aagaactggg
     1981 atgggagctg gggcgtaccg cccatatctg gaccgctttt cttgtggcgt atatgctggg
     2041 tcagttcatc agcagctatg cggggcggcg atggggcccg cgcctgcttt tgctgggcgg
     2101 tctcggcatc tcccttcttt gcaatctcat cttcggattc gccaattcct actatacctt
     2161 tctggtcttc atggccttca acggttttgt gcaggcagca ggctggccgg gttgtgtcgg
     2221 cgctatttcg gaatggcttc gggtgaaaga acgggggcgc atcttcggca tctggacgac
     2281 caactacatc gtaggcagcg tcatggtcaa aacgctgacc ggcatgttcc tcggtatgac
     2341 cgggctgtat ctggggctgg cgggctggcg gtgggccttt ttcggctgta cggcactcgc
     2401 ttttttactc tggtggcttc tttatttctg gcagcgcacc cgtcccgaag atgtgggtct
     2461 gacccctttg gttaccgaag aggaaacaga gcgccagacc attcatgcat ccaaagaaga
     2521 ccaggtgggc atgaaggatt atcttattct tgcgctcaat cctctggtgc tgaccatggg
     2581 cggcgcctac ttctgcgtca agttcctgcg ctacgcactg gattcctggc tcccgtcctt
     2641 cctcattata caggggctgg atccttctct gtcgagttat tattccatgg gctttgacat
     2701 cgccggtctt gccggttccg tcgccacggg ctacatgctc gacaagtggt tcaaaggcaa
     2761 ctgggcggcg ctttgtctgg ttctgagtct cggcgtcatc ggcggctacc tggcagtcat
     2821 gtatctcggt gcttctccga tagccattgc tctgtgtttc agtattgtgg ggttcatgct
     2881 ttacggtccc gatattcttc tgagcggtgc cggtcctgtg gagatcgccg gtgcgcggaa
     2941 cggcatggta gtagcaggca ttgtgaacgg actgggaagc atcggtccca ttgtgcagga
     3001 ggaagtgatc ggcaggctgg tccgcggcga tgtgcagcag ggcatggcaa acacaaaccg
     3061 tctggctctg gggatcagcg tgctgctgat gctgcttttt atccccatgg ttatccgcct
     3121 gcagattgcc cggcgcaaaa gaagttaatg tgcttgtgcg atgccttctg acggggcaaa
     3181 ccagcgattc ataactgcgg caatttcttc aggtttttcc tgagccagaa aatggccggc
     3241 gcccgggata acctgcattt ctgatccggc aatgcgtttg tgctgctctt ccacatactg
     3301 ctgcatatag ctgttctcgc cggtaatcag cagcacgggc atctggagct gcggcagacg
     3361 gctcataatt tcggtcttgt ccgacatctc atatacccat gcctcgatat cgcccgggca
     3421 tttgagcagg atctcttctc cgtcaccctg aaaaaagcag tatttcagat agcagtcgag
     3481 gctgtcgccc gtccacatat tcatgggttc cttggcactg aagcgttcgc gtgcttcctg
     3541 ccgggaagaa aaacggctgc gccgtctcct cacgagtttt gccagcagtt ttgctcctgc
     3601 gaaaaaatcg ggcagtgcaa taatggcatc gacaagcatc agccgcgaga atgtgccggg
     3661 gcgtatcagt tcggcctgta caatggtcga tccgccgccg ctgtgcccca ctgcctgcat
     3721 cccttcgggc aaccccatct gatcaaaaac gccgagcaga tcccgggcga ggctgtccca
     3781 ggtataggct tccgcttcag gacttttggc ggaatcgcca tgcccgcgag cgtcccaggc
     3841 gaagagggta tggttcagac tgagggttcg aaagacagac tcccagactc tgccgctcac
     3901 gcctgtacaa tggcagaaaa acagaggtgt gccgtcgccg ggatattccc aaacactgaa
     3961 gtcggcatta cgggtccgca ctatgtgctg cttcggggtc agagtacgca cagatatttc
     4021 ttccggtggt catgccattg ccggagcaaa gccgagtact tccacaatct gctcttccac
     4081 agcttccaca gtccggggct gcgacgccgc cgcaatggcg ttatccgggt ctttcagacc
     4141 gtgtcctgtc aggatacaca cggcccggag catattgcct gtgcggggcg tgcattcgtc
     4201 gaaatatccc gccttggaca gcttcacaag gcccgcaatg cttgcggcgc tggcgggttc
     4261 gcagaagatc ccctccgttc tcgccaccag cgtataagca tcccggattt catcatcagt
     4321 caccatatca atgacaccgc ccgattcatc acgagccgct tccgctgttg tccagcttgc
     4381 gggattgccg atgcggattg ctgtggcgaa ggtttcgggc tggagcaccg gatgacccag
     4441 cacgatgggc gccgatcccg ccgcctgaaa gccgagcatg cgcgggcggc cttcgcattt
     4501 gcccatttca tgatattcct tatatccctt ccagtaggcg gtaatgttgc ctgcattgcc
     4561 cacgggcatt gcctgaaaat cgggcgcgat tccgtcgaaa tcttccagaa tttcaaaggc
     4621 gccggtcttc tgcccttcga tacgataggg attgacggag ttgaccagag cgatggggaa
     4681 gttgttgcac atgcgccgta ccagattcag tgcttcatca aaattgccgc ggatctgaat
     4741 cacctgcgcg ccatggatca tcgcctggct gagctttccg aaagcgattt tcccctccgg
     4801 aatcagtacc acacatttga tgcctgcccg ggcggcatag gcggcagcgg aggcggaggt
     4861 gttgcctgtg gaggcgcaca tcaccatttc gaaaccttct tccacggcct tggtgatcgc
     4921 catggtcatg ccccggtctt taaaagagcc ggtcggattg agcccttcat atttcaggta
     4981 aatttcgagg cggggatgaa tagccgcact caaaccgggc gccgccacca gcggtgtgga
     5041 tccttcgttc agcgtaatga tgcgtgtggt atctgacaca ggcagatgcg aacgaaagcg
     5101 ggcaataagg ccggggttgt acatcgggga tgcctttcgt tgaattagaa aacgcgaagg
     5161 aattgcgtgg gttcagcgat acaatcaaaa ccgtctattt cggacagggc ggcttttatg
     5221 gccgattcaa ccgtggcatg ggtcatgagc accacatgca ccggttcctg atcgccctga
     5281 tttttctgta tgcaggaagc aatgctgaca ccatgtttgc ccagtgtgga gcagacacgt
     5341 cccagcacgc cgggatggtc ttccgtagtg aggcgcagat aatagcagcc ctcagcaaga
     5401 ccgatgtccc gaatcgccag attgcgcgag taggtaaaag gtttgaaagg agcgcagcct
     5461 ttgttccggg caatatccac aatatcgccc acgacggcac ttgctgtagg cttccgccct
     5521 gcaccgcgtc cgtaatagag ggtcgcatcg gcacagtcgc tttttacata gatgccgttg
     5581 aactcgttgc gcacggcggc cagcagatgg ctctcgggaa tcagcgccgg atgaacccgc
     5641 gcttcaatac tgccgtccac ttcacggata atggcgagca gtttgatgat gtagcccatt
     5701 tcccgtgcat acgccacatc gagatgggta atcgaggtga tcccttccac gggaatcgca
     5761 ttcagatcaa ttcgggtgct gtatgccaga gaagccagaa tctgacattt gtgtgccgta
     5821 tcatggccca gaatatcgag atccggcggt gtttcggcaa aaccgttggc aatcgcctga
     5881 ttgagagttt cttcaaattc aagaccttca taggtcatcc ggctgagaat ataattgcag
     5941 gtaccgttga gaatgccgta tatgccttca atccggtttg ccgccagact ttcgcgcagt
     6001 gctttgatga tcggtaccgc accggcaaca gcggcttcaa agcgaagttc cacgccatgc
     6061 tgtactgccg cttccaccag ttccgg
//
LOCUS       DAOPWB010000167         6046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_12995_length_6046_cov_4.847772, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000167 DAOPWB010000000
VERSION     DAOPWB010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12995_length_6046_cov_4.847772"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKN92_11870"
     CDS             complement(<1..426)
                     /locus_tag="PKN92_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNZ49530.1"
                     /translation="MKKWVKRLLAAVLVLFFLLWTAVTAGSVQLTTRPRTRMIEDIST
                     LAERPVEAVTIITEDDVTLSAWYVPAESDRAVVLLAGIDANRTSSIGRGIFFLERGFA
                     VLLPDLRASGKSGGKRVTIGWQERLDLKACCRFLSERGYR"
     gene            637..1137
                     /locus_tag="PKN92_11875"
     CDS             637..1137
                     /locus_tag="PKN92_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNZ49531.1"
                     /translation="MSTKDNLQEAFAGESQANRKYLAFAKKAEQDNFKQVARLFRAAA
                     AAETVHAHAHLRAMGGIRGTLENLEEAKGGEEFEFNSMYPGFVAEAEAEGNKAALISF
                     KNAMAVENIHAGLYGEAIEAVKAGKDLPETPIYVCEICGNTVYGEAPDKCDICGVPKE
                     RFFAVE"
     gene            1232..2314
                     /locus_tag="PKN92_11880"
     CDS             1232..2314
                     /locus_tag="PKN92_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNZ49532.1"
                     /translation="MVPHHHDKRLDVLRTRLGVWGADVFISFDPADNAWLTGFFGSTS
                     IVAVTAEEAWLFCDFRYLEQARASVTAAQVVEFSGAAEPFLARHLNAFCLKQAVCDPS
                     VMTLACYNRLKEHYEGELTAQPDVCRALRTVKEEGEIARIEKASLLAEDALQAVLGEI
                     RPGMEETELAGRLDYAFRQRGAQRNSFDTIVLFGEGSSLPHGKPGNRGLQPGDIILID
                     CGCVLDGYCSDLTRTFVYKQSPGAWFQEIYDCVYQAQAAALAAVRDGISARTVDETAR
                     TAIKNSGFGDYFGHGTGHGVGLEIHESPRLNPESEAILAAGMVVTVEPGIYLPGRGGV
                     RIEDLLVVTPSGSRVLTVLPKELRIL"
     gene            2317..2874
                     /gene="efp"
                     /locus_tag="PKN92_11885"
     CDS             2317..2874
                     /gene="efp"
                     /locus_tag="PKN92_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNZ49533.1"
                     /translation="MISTADFRNGMVVELDNDLMEIVEFQHVKPGKGGAFVRTKFKNV
                     LTGRVLEKTFRSGEKFEEARIETRKWQFLYSDGEQFHFMDHETYEQLAVDADVVGDQA
                     LWIKENSDASLIFYKGNVISVEAPAHVVLEITKSDPGVQGDRSSGAKKPATLETGATI
                     QVPLFLNEGDHVKVDTRTGEYLERM"
     gene            3060..>6046
                     /gene="carB"
                     /locus_tag="PKN92_11890"
     CDS             3060..>6046
                     /gene="carB"
                     /locus_tag="PKN92_11890"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNZ49534.1"
                     /translation="MPRREDIEKIMIIGSGPIVIGQACEFDYAGTQACKALRGLGYKT
                     VLVNSNPATIMTDPVMADATYIEPLNVPTLRRIIEKERPDALLPNLGGQSGLNLSLEL
                     ARQGVLDEFNVQMIGVNVDAIERGEDRRTFKEVMKGIGVDVPRSELAYSVEEAEKIAA
                     DLGYPVVVRPAYTMGGTGGGLVYNLQELQTITARGLAASLVGQVLIEESVLGWEELEV
                     EVIRDAKNQIITVCFIENVDPMGVHTGDSFCVTPMPTVSDALKERLEKTAHAIVEAIQ
                     VIGGANVQFAHDPASDRLTVIEINPRTSRSSALASKATGFPIAMISALFAAGLTLDEI
                     PYWRGGTLDKYTPSGDYVVVKFARWAFEKFKGIEDRLGTQMRAVGEVMSIGKSFKEAF
                     LKAIRGLESGRNGLGFAKNFHQLPLEELLALVGTPTSERQFQLYEALRKGACVDDLYK
                     RTRINPWFLQQMKELVELEEEILKHKGNLPPDSLLVQAKKDGFSDKYLAQLLTLSEKQ
                     IREKRLALGVSEAWEPVPVSGVENAAYYYSTYNCPDKIPAAEGKKVMVLGGGPNRIGQ
                     GIEFDYCCVHAAFSLRDQGYESIMVNCNPETVSTDFDTSARLYFEPLTVEDVLSIYEK
                     EKPLGVIVQFGGQTPLNIAGELEKAGVPILGTSVETIDLAEDRDRFRHIIEELGIPQP
                     KSGMASNLDQALAIAAEIGYPLMVRPSYVLGGRGMEVVYDEEMLRRYVAAAVEITPER
                     PILIDKFLDNAIEVEADAISDGESAFVPAVMEHIELAGVHSGDSACVIPPISVTPRQV
                     ETINEYTRRIAVALKVVGLMNIQYAIADDIVYILEANPRASRTVPLVSKVCNIPMASL
                     ATQVMLGKKLGELGLYQKTIPHYGVKEAILPFNMFPEVDPLLGPEMRSTGEVLGMANS
                     FGIAFFKAQEATQLKLPTEGTVLITVAENDKPAVLEAARLLSKNGFRVLATSGTAAFL
                     RTNNIPCEEVLKMHEGRPNLMDT"
BASE COUNT         1429 a         1510 c         1720 g         1387 t
ORIGIN      
        1 ccgatatccc cgctctgaca gaaaacggca gcatgccttc agatccagcc gttcctgcca
       61 gcctatggta acacgcttgc caccgctttt tccggaggcg cgcaggtcgg gcagcagcac
      121 ggcaaagccc cgctccagaa aaaaaatgcc gcgccctatg ctggaggtgc ggttggcatc
      181 gatgcctgcc agaagaacca cggcgcggtc actttccgcc gggacatacc aggcagaaag
      241 cgttacatca tcttcggtga taatagtgac cgcttccacg ggacgctccg ccagggtgga
      301 gatatcttct atcatccgag ttctgggccg tgtcgtaagc tgcacacttc cggcggtaac
      361 ggcagtccat agcagaaaaa aaagtaccag tacggcggca agaagtcttt tcacccattt
      421 tttcattgcg ctgccctcca gcccggtacc ccttcaaggg gtagtcccac cggtaaaact
      481 attgtaaagc agaagagaga aaaattcaaa aaatcgtgta ttttcaaagt ttagcacttt
      541 gtgaagttcc gggataattt gcacaaagtg ccgtactatt agtatcatat tagccgagga
      601 ataccctttt accccctgtt catgaaagga agaactatga gcacaaaaga caaccttcag
      661 gaagcctttg ccggtgagag ccaggccaac cgcaagtatc ttgccttcgc caaaaaggcg
      721 gagcaggaca atttcaaaca ggtggcgcgt cttttccgcg cggcagccgc tgccgaaacg
      781 gtgcatgccc atgctcacct tcgtgctatg ggcggtattc gcggtacgct tgaaaatctg
      841 gaagaggcta aagggggaga ggaattcgag tttaattcga tgtaccccgg ttttgtggcg
      901 gaagcagagg cggaaggcaa taaagctgcg ctgatcagtt tcaaaaatgc catggctgtc
      961 gaaaatattc atgccggtct ctatggcgaa gccatcgagg cggtgaaggc aggaaaagat
     1021 cttcctgaaa cgcccattta tgtgtgtgaa atatgcggca atacggttta tggcgaagcc
     1081 ccggataaat gcgatatctg cggtgtgccg aaagaacgct tctttgccgt ggaataaata
     1141 attccgggac agtgtaacgc tatgggctgt atgtgccgtc ctgtacgaat cggaaacaac
     1201 ggcaggtgct gcagcgtaaa ttgagaaaac aatggttccc caccaccatg ataaaaggct
     1261 tgatgttttg cgcacacgcc tcggcgtgtg gggcgccgat gtttttatca gcttcgatcc
     1321 ggcggacaat gcctggctga ccggtttttt cggaagcacg tccatagtgg ctgttacggc
     1381 ggaagaggcc tggctgttct gtgatttcag gtatctggag caggcgcgcg catcggttac
     1441 agcggcgcaa gtcgtggaat ttagcggtgc agcagagcct tttcttgccc ggcatctgaa
     1501 tgcattctgt cttaagcagg ctgtctgtga tccctctgtc atgacgcttg cctgttataa
     1561 caggctcaaa gagcactatg aaggcgaact gacggcgcag ccggatgtct gccgcgccct
     1621 tcgcactgta aaagaagaag gcgagatcgc ccgaattgag aaggcaagtc ttctggcaga
     1681 agatgcactc caggctgttc ttggtgagat caggcccggt atggaagaaa cggaattggc
     1741 gggtcgcctg gactatgctt ttcggcagcg cggggcacag cgcaattcat ttgatacgat
     1801 cgtgctcttc ggtgaaggca gctcactgcc ccacggtaag cccggaaacc gcgggctgca
     1861 gcccggagac attatcctca ttgactgcgg atgtgtactc gatggctact gctccgatct
     1921 gacccgcacc tttgtgtata agcaatcgcc gggagcatgg tttcaggaaa tttatgactg
     1981 cgtttatcag gcacaggcgg cggcgcttgc cgctgtccgt gacggaatat ctgcccggac
     2041 agtagatgaa actgcccgaa cagcgataaa gaacagcggt ttcggtgatt atttcggtca
     2101 tggcaccggt catggtgtcg gacttgagat tcatgagtct ccgcggctca accccgagtc
     2161 ggaagccatt ctggctgccg gcatggttgt cacggtggag cccggtattt atctgcccgg
     2221 cagaggcggt gttcgaatag aagatttgtt ggtggttacc ccttcgggga gccgtgtgtt
     2281 aactgtttta ccaaaggaat tacggattct ctgagtatga tttcgacagc agattttaga
     2341 aacggcatgg tagtggaact ggacaacgac ctgatggaga tcgttgaatt tcagcatgtg
     2401 aaaccgggta aaggcggcgc cttcgtccgt acaaaattta aaaatgtact gacgggcaga
     2461 gtgctggaaa aaaccttccg ttccggtgaa aaatttgaag aagcacgcat tgagacgcgt
     2521 aaatggcagt tcctttacag cgatggcgag cagtttcatt tcatggatca tgagacctat
     2581 gaacagctgg cagtcgacgc tgatgtggtt ggagatcagg cgctttggat caaggaaaac
     2641 tccgatgcgt ctctcatttt ttacaaagga aatgtgatca gcgtcgaagc accggcgcat
     2701 gtggttctgg agattaccaa atccgatccc ggtgtgcagg gcgaccgttc cagcggagcc
     2761 aaaaagcccg ccacccttga aacaggagca accatacagg taccgctttt cctgaatgaa
     2821 ggtgatcatg ttaaagtgga cacacgcacc ggcgaatatc ttgaacggat gtgagctcgg
     2881 ggcgattatg cctgcttttt tccgaatgca gttttgaatc gatttatcat acagtattga
     2941 aatatcaatc cggatgctca ggcatttcat cagcacactg cagagcgcag cagccggatt
     3001 aacgggaggg cgaacgctgt ttgccctttt tctatgtatc agcacgaaag gataatacca
     3061 tgccgcgccg ggaagacatc gaaaaaatta tgataatcgg ctccggacct attgttatcg
     3121 gtcaggcttg cgaattcgat tatgcgggca ctcaggcgtg taaagcactg cgcggtctgg
     3181 gctacaaaac agtgctggtg aattccaatc ccgccacaat catgacagat cctgtcatgg
     3241 ccgatgccac ctatatcgag ccgctgaatg tgcccacgct ccggcggatc attgaaaaag
     3301 agcgtcccga tgcccttctc cccaatctgg gcgggcagag cggcctgaac ctttctcttg
     3361 agctggcgcg acagggcgtg ctcgatgaat tcaatgtaca gatgattggc gtgaatgtgg
     3421 atgccattga gcgcggagaa gaccggcgta ccttcaagga agtcatgaaa ggcatcggtg
     3481 ttgacgtgcc gcgcagcgag ctcgcgtact cggttgagga agcggagaaa attgccgctg
     3541 acctgggcta tcctgttgtg gtgcgccctg cctataccat gggcggaacg ggtggcggtc
     3601 tggtgtacaa cctgcaggaa ctgcagacca ttacggcgcg gggactggct gcaagccttg
     3661 tagggcaggt gctgatcgaa gaatctgtcc tcggctggga agagctggaa gttgaggtga
     3721 tccgggatgc caagaaccag attattaccg tgtgtttcat tgaaaatgtg gatcccatgg
     3781 gagtgcatac cggtgattcc ttctgtgtta cacccatgcc caccgtctcc gatgcgctga
     3841 aggagcgtct tgaaaaaacg gcgcatgcca ttgttgaagc cattcaggtt atcggcggtg
     3901 ccaacgtgca gtttgcccat gaccccgcca gtgatcggct gacggttatt gaaatcaatc
     3961 cccgcacctc ccgttccagt gcgctggctt ccaaggcaac gggctttccc attgcgatga
     4021 tctccgcact ttttgccgcc ggactcacgc tggatgaaat cccctactgg cggggcggca
     4081 cgcttgacaa atatacgcct tccggtgatt atgtcgtggt aaaattcgcc cggtgggcct
     4141 ttgaaaagtt caaaggaatt gaagaccggc tcggcacaca gatgcgtgcg gtgggcgaag
     4201 tcatgagtat cgggaaaagc ttcaaggaag ccttcctcaa ggcgattcgc ggtctggaaa
     4261 gcggaagaaa cgggctgggg tttgctaaaa attttcacca gttgcctttg gaggaactcc
     4321 tcgcactggt gggcacgccc acgagcgagc gtcagtttca gctgtatgaa gcccttcgaa
     4381 agggtgcctg tgttgatgac ctttataaac gcaccaggat caacccctgg ttccttcagc
     4441 agatgaagga actggtggaa ctggaagaag agattctgaa gcacaaaggg aaccttccgc
     4501 cggacagcct tcttgttcag gcgaaaaaag acgggttcag cgataaatat ctggcgcagc
     4561 tgcttacgct ttctgaaaag cagatccgtg agaaacgcct ggcgctgggt gtgtccgaag
     4621 cctgggagcc ggtgccggtg agcggtgtgg aaaacgccgc ctactattat tccacttata
     4681 attgcccgga taagattccg gcggctgaag gcaaaaaagt gatggtgctg ggcggcggac
     4741 ccaaccgtat cggccagggc atcgaattcg actactgctg cgttcatgcg gctttctctc
     4801 tgcgcgatca gggctatgag tccattatgg tcaactgcaa ccctgaaaca gtgtccacgg
     4861 attttgatac ctcggcgcgt ctgtactttg agccgttgac cgtggaggac gtgctcagta
     4921 tttatgaaaa agaaaagcct ctgggcgtaa tcgtccagtt cggcgggcag acgcctctca
     4981 acatagccgg cgaacttgaa aaagccggcg tgcccattct gggtacttct gtggaaacca
     5041 ttgaccttgc ggaagatcgt gaccgcttcc gtcatatcat tgaggagctc ggtattcccc
     5101 agccgaaatc gggtatggcg agcaatctgg atcaggcgct tgccattgcc gctgaaatcg
     5161 ggtacccgct catggttcgg ccttcctatg tgctgggcgg cagaggtatg gaagtggttt
     5221 atgatgagga aatgctccgg cgctatgtgg cggccgccgt ggaaatcacg cctgaacggc
     5281 ctatcctcat tgataaattt ctggacaatg ccatcgaagt ggaagcggac gctatttctg
     5341 acggggaaag cgcttttgtt ccagctgtca tggagcatat tgagctggca ggcgtgcatt
     5401 ccggcgactc cgcctgtgtg attccgccta tcagcgttac cccacgccag gtggagacca
     5461 tcaacgaata tacacgtcgt attgccgtag cactcaaggt ggtgggactc atgaacattc
     5521 agtatgccat tgccgatgat atcgtctata ttctggaggc gaatccccgt gcgtcacgga
     5581 cggtacccct ggtttccaaa gtgtgcaata ttcccatggc gtcccttgca acgcaggtca
     5641 tgctcgggaa gaaactgggc gagctcggac tttaccagaa aaccatccct cattacggtg
     5701 tgaaagaggc gattcttccc tttaatatgt tcccggaggt ggatccgctg ctgggccctg
     5761 aaatgcgttc taccggtgaa gtgctgggca tggcgaattc tttcggtatc gcttttttca
     5821 aggcgcagga agccacgcag ctgaagctgc ccacagaggg gactgtgctt attaccgtgg
     5881 cggaaaatga caagcccgcc gtgctcgaag cagcgcggtt gctgagcaaa aacggattcc
     5941 gtgtgctggc gaccagcgga acggcagcct tccttcgtac caacaatatt ccctgtgagg
     6001 aagtattgaa aatgcacgaa ggacgcccca atctcatgga tactat
//
LOCUS       DAOPWB010000168         6018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_13090_length_6018_cov_3.913969, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000168 DAOPWB010000000
VERSION     DAOPWB010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13090_length_6018_cov_3.913969"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PKN92_11895"
     CDS             complement(<1..191)
                     /locus_tag="PKN92_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49535.1"
                     /translation="MTTILLCLSMSLLTVAAADEAPGLAVMSYNIHHAEGMDGKIDLD
                     RIAAVVLESGADIVCFQEV"
     gene            350..835
                     /locus_tag="PKN92_11900"
     CDS             350..835
                     /locus_tag="PKN92_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNZ49536.1"
                     /translation="MNKWLYEGVQFEYHYDMKLSTRARYGLRAMVELARHDDGITGRQ
                     IAENQNLPVAYLEQLLSRLRHAGLVKATRGARGCFMLARPAETISLAEVIEALEGVIE
                     MASCDEIAHCSNDPDHCALREFYEGINKTLYEHLSGISLQKLMQDHLQSLVDSSDDFS
                     I"
     gene            964..2364
                     /locus_tag="PKN92_11905"
     CDS             964..2364
                     /locus_tag="PKN92_11905"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNZ49537.1"
                     /translation="MSLLKCTQKSTVLALLLCLLTPAALQAADEPALFQVGRARECIT
                     PALGAKLAGYFHDREAKYVRDDLFARAVVIDNGEAQLALVSCDLISMTAEVSVPAKER
                     VEKETGIPAANILVCATHTHTGPELRPNGSVARDEVWTKELIDHIANAVIHAHQSKVP
                     ARLCVGQADASGYSFNRIFRRKDGSEVFGTWEKGAPILGAAGPVDPVLQTLSAVDESG
                     KLLAVMVNFAMHPDVIGGGSADFISADWPGLMGNALSAVYGDDMVTLLLQGTCGDINH
                     RPHDKTFLPTSGPKKAEQLGRALAGAAMVALERAEPMVDSHLAVMTKVLDLPYYTRDE
                     SIFKEMEALEKKENLSYLEEANIRRVREWANDGKSIAVPVQTMRIGDVGLAAFPAEIF
                     VRLGLEVKEWSKAPHTFVVELSNDRVSTYVPDTDHADRGAYGQKPILSRWLRSDAGRH
                     MADAAIDMLYDLYTAE"
     gene            2845..>3030
                     /locus_tag="PKN92_11910"
     CDS             2845..>3030
                     /locus_tag="PKN92_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49538.1"
                     /translation="MTTTDTAAQDQKDYPEQEQTFGEKLLQTIDTLPDDTVTLVEIRD
                     LIGQEGLLLFTIFLNLIF"
     assembly_gap    3031..3040
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3041..3508
                     /locus_tag="PKN92_11915"
     CDS             <3041..3508
                     /locus_tag="PKN92_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HNZ49539.1"
                     /translation="PGVSTVFGAAILLVGLFRFFNRPFWLPGFIATRRLPSAKLRSAL
                     QKSTIWIGRLEKISRPHTLTWFTRGGLAALSNNGGLILGAVLLMMPFGLVPFSNTLPA
                     LACILLAVGMLQCDGRCILLGHVTNVLTIIYFAVLIIIFILAGDLALSSFFKG"
     gene            3525..5642
                     /locus_tag="PKN92_11920"
     CDS             3525..5642
                     /locus_tag="PKN92_11920"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNZ49540.1"
                     /translation="MENRHVLFYVFFAALLLLLPVCGQAADVTLYRDTWGVPHIYGDS
                     WANAAYALGQAQAEDRLEDIYKNVRTAMGTMAEAFGEDYAEQDFFLRVFKNAELCEKY
                     WDTAPAHIREICDSFMRGVEDYVAEHPEKKPECACDLYGWQCVAVGRTMLLNWPLGVL
                     NDEVHRKKEAPAFGSNAFAVAPSRSAEGCPILMTDPHLEWEGLAVFYEARLHTDSEDL
                     CGFFIVGSPAPVLGHNAHVAWACTTGGPDTSDVYMVKTNPYGFMQYEYNGEWLVFDAD
                     IIRIKVKDAPTQYKPALYSVYGPLLDLPDLKRNIAYCGATPYFDQVAMMEQMYNMATS
                     RNCEEFYNALAMNQFMEQNILFADRDGNIQYVRTGRTPIRPEGDINWSVPVPGGSDAT
                     RWLGIHNIKDLVQIKNPPQGYFQNCNVSPAVMMSDSPMTPDKYKDYIFNVSWDQNSPR
                     GRRLLQLLDADPLITKDAAIAYTMNNYDLLAVPWQTALAAAVAARGEDNPPEEELARA
                     VQGILRWDGYFSKEEPFTFLYKRWRLKCQDAIDIAAIADGEPLGAEDQEKMLSLLAEA
                     QQEMKEKYGSFEVPWGDLVKIGRGGRYFPLECADFGGGKDKKNQTETVLDVASKEMPD
                     SDGKLVGHNGSGSLMIAFLHKDGIESHSLVAWGQSADPESPHYVDQSEKLYSPRKLKP
                     TWFTKEELMEHLESTRTITVPGR"
     gene            complement(5645..>6018)
                     /locus_tag="PKN92_11925"
     CDS             complement(5645..>6018)
                     /locus_tag="PKN92_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49541.1"
                     /translation="SSYDTLTSAVANRLAVFYTPHFIAYPFYAGFALLTGANPFAMRP
                     VDFMKYAVMPTLILAGDAEIEIPVHETQSLYDRCPAALKKLHFFHGAGHWNFLSRYTD
                     EFTSVVDLFLAEVFPAETDAG"
BASE COUNT         1370 a         1515 c         1694 g         1429 t
ORIGIN      
        1 tccacttcct gaaagcatac gatatcggcg cctgattcca gaacaacagc ggcgatacga
       61 tccagatcga tttttccatc cattccttcg gcatgatgga tgttgtagct cattactgca
      121 agaccggggg cttcgtctgc agcagcaacg gtgagaaggc tcatggataa acacaggagg
      181 atagtagtca ttatgaaaat tccctgatta tgttttatgt tctttgcctg tttcagacag
      241 ggcgttcttt gtttagcctg ttaaaatcat accccgtagc cacagtggcg tacaacctcc
      301 ggcattattg ttttcggggg tatcgaagaa agtgcactgt gagcagggca tgaataaatg
      361 gttgtatgag ggagttcagt ttgagtatca ttatgacatg aaactatcca cacgcgcacg
      421 atacggcttg cgtgccatgg tcgaactggc acgccatgac gacggcatta caggaaggca
      481 aatcgcagag aatcaaaatc tgcctgttgc ttatctggag caattgctgt cccgtctgcg
      541 ccatgccgga ctggtcaaag cgacccgggg tgcccggggg tgcttcatgc ttgcccggcc
      601 agcggaaact atttctctgg ctgaagtgat tgaagccctt gagggggtaa ttgaaatggc
      661 gagctgcgat gagattgctc actgctccaa cgatcccgat cactgtgcat tgcgcgagtt
      721 ctatgagggg atcaacaaga cgctttatga gcatctgtcc ggcatatcac tgcaaaagct
      781 gatgcaggat cacctccagt ccctggtgga ttcttcggac gatttctcga tctgatcttt
      841 tcgaagagca ccggtttcat cttgcacaga ccggcgactg cagtatggcg ggaatcataa
      901 aaaactttac tccacatact tcttttatca ataatgacag aaagattgaa aaggagtact
      961 gccatgtcgc tgttgaaatg cacgcagaaa agcactgtcc ttgctcttct tctttgcctg
     1021 ctgacccccg ctgctcttca ggctgccgat gaacctgccc tttttcaggt aggccgtgcg
     1081 cgggaatgta tcactccggc actcggcgcc aaacttgccg gctattttca cgaccgggaa
     1141 gcgaaatatg tgcgcgatga tctttttgcc cgtgctgtag tcattgacaa cggcgaggca
     1201 cagctggcac tggtttcctg cgatctgatc agtatgaccg ctgaggtgtc cgttccggca
     1261 aaggagcggg ttgaaaagga gacggggatt cctgccgcca atatcctggt ctgcgctacc
     1321 catacgcaca ccggccccga gctccgaccc aacggcagtg ttgcacggga cgaagtgtgg
     1381 acaaaagagc ttatagacca catagccaat gcagtgatcc atgcgcatca gtcgaaagtg
     1441 cccgcacggc tctgtgtggg acaggcagat gcttcgggct attccttcaa tcgcattttc
     1501 cggcgcaagg atggaagcga ggtcttcgga acgtgggaga agggagcccc cattctcgga
     1561 gctgccggcc ctgtggatcc ggtgctgcag acgttgtcag ccgtagatga gagcgggaaa
     1621 ttacttgcgg ttatggttaa ttttgccatg catcccgatg tgatcggtgg tggcagtgca
     1681 gactttattt cagcagactg gcccggtctc atgggtaatg cgctctctgc ggtttacggc
     1741 gatgacatgg tgacgcttct gctgcagggt acttgcggcg atatcaacca tcgcccccac
     1801 gataaaacct ttctgcccac ctcaggaccg aaaaaagcgg agcagctggg cagggccctg
     1861 gcaggggcgg caatggttgc cttggagcgg gcggaaccga tggtggattc acatcttgcc
     1921 gtaatgacaa aagtgcttga cctgccttat tacacccggg atgaatccat attcaaggaa
     1981 atggaagcgc tggagaaaaa agaaaacttg agctacctgg aagaggcgaa tattcgtcgg
     2041 gtgcgtgagt gggcgaatga tggaaaaagc attgccgtac cggtgcagac catgcgtatc
     2101 ggcgatgtgg gactggctgc ttttccggct gaaattttcg tgcggctggg ccttgaagtg
     2161 aaagaatggt caaaggcgcc gcataccttt gtggtggagc tttccaatga ccgggtttcc
     2221 acttacgttc cggacacgga tcacgccgat agaggcgcct acgggcagaa accgatcctg
     2281 tcccggtggc tccgctccga tgcaggcaga catatggcgg atgctgccat cgatatgctc
     2341 tatgaccttt acacagccga atgacaggat cgcctccggg aagagaagag gttccaaagc
     2401 aggctgcaaa atgggttgat gcgttaagcc ccggatgagt tgacgccggg tgtctctttt
     2461 ttttcgcatg gtctttttgc aatgagaggc gggcagtgcc cgcactgcga caagtagttg
     2521 taaaaaaggg ttgctggtct ttccggcttc ctgatccgct aatcggatca acctgcgccg
     2581 gggcagcgtt gcaacaagga gcgaactttt ttcgtgcgtg ttcgctcttg cgtgcgggaa
     2641 tgtttcgggc atggtggtct tatgcctgta caggcttccg atgtcgctcc tgccggggtg
     2701 cacaaaaaaa acggtttgat ctttcagtgc ctttttactt agagtagtgg ggtacgttta
     2761 acttgtacaa atccgtatca aacatggtat gcaggcattt ttctgtctcg ctgccgtatg
     2821 tatcaggaag gccggacccg ccctatgacc actacagaca cagccgctca agaccaaaag
     2881 gactatccgg aacaggaaca gacctttggc gaaaaacttc tgcagaccat cgacaccctt
     2941 ccggacgaca ccgtgacact ggtggagatc cgtgatctga tcgggcagga agggcttctg
     3001 cttttcacga tttttctgaa tctcattttt nnnnnnnnnn ccgggcgtga gcactgtatt
     3061 cggtgcggca attctgctgg tggggctttt ccggtttttc aaccgcccct tctggctgcc
     3121 cgggtttatc gccacgcgcc gcctgccttc cgctaaactc cggagcgcac ttcagaaaag
     3181 tacgatatgg atcggtcgtc ttgaaaaaat cagccgtccc cacaccctca cctggtttac
     3241 ccggggtgga cttgctgccc tctcaaacaa cggcggtctc atactgggcg ccgtgctgct
     3301 catgatgcct ttcggcctgg tgcccttcag caacacgctc ccggcgcttg cctgcatcct
     3361 tctggccgtc ggcatgcttc agtgtgacgg acgctgtatc ctgctgggac atgtgaccaa
     3421 tgtactgacc attatttatt ttgctgttct gatcataatc ttcattcttg ccggtgatct
     3481 ggcgctcagt agttttttta aaggataagg aaggatcgtt tccgatggaa aacaggcatg
     3541 ttcttttcta tgtgtttttc gctgcgctgt tgctgctact gcctgtgtgc gggcaagccg
     3601 ccgacgtgac cttgtaccgg gatacctggg gtgtgcccca catttatggt gacagctggg
     3661 caaacgcagc ctatgccctc ggtcaggcac aggcggaaga ccgtctcgaa gacatctaca
     3721 agaacgtacg aacggcgatg ggcaccatgg ctgaggcttt cggtgaagac tatgccgagc
     3781 aggatttttt cctgcgtgtt ttcaaaaatg ctgagctatg cgaaaagtac tgggatacag
     3841 cgcctgcgca tattcgcgaa atctgcgaca gttttatgcg cggtgtggaa gactatgtgg
     3901 cggaacatcc ggagaaaaaa ccggaatgtg cctgcgatct ttacggctgg cagtgtgttg
     3961 ccgtgggaag aaccatgctg ctgaactggc ccctcggtgt tttgaacgat gaggtgcaca
     4021 ggaagaaaga agcgcccgct ttcggatcca acgcttttgc cgtggcgccg tcccgttccg
     4081 ccgaaggctg ccccattctt atgaccgatc cgcatctgga atgggagggg cttgccgtgt
     4141 tttatgaagc ccgactgcat acagacagtg aagatttatg cggctttttc attgtaggtt
     4201 ctcctgcacc tgtcctcggg cataatgccc atgtcgcctg ggcctgcacg accggcggcc
     4261 cggatacttc cgatgtgtac atggtcaaaa ccaaccctta tggattcatg cagtacgaat
     4321 acaacggcga gtggctggtc tttgatgcag atatcatcag gatcaaagtg aaagatgcgc
     4381 ccactcagta caagcccgca ctgtattcgg tctacggtcc gctgctggat ctgcccgatc
     4441 tgaaaagaaa cattgcctac tgcggtgcca ctccgtattt tgatcaggtt gccatgatgg
     4501 agcagatgta caacatggcg acgtccagaa attgcgaaga attttacaat gctcttgcca
     4561 tgaatcaatt catggaacag aatattcttt ttgcggaccg ggacggcaat attcagtacg
     4621 tccgcactgg acgcacaccg atccggccgg aaggcgatat caactggtct gtgcctgtgc
     4681 cgggcggctc cgatgcgacg cgctggctcg gtattcataa tattaaggat ctggttcaga
     4741 tcaaaaatcc gccgcagggc tatttccaga actgcaatgt cagcccggct gtgatgatgt
     4801 ctgactcgcc catgaccccc gataagtaca aggactatat cttcaatgtc agctgggatc
     4861 agaacagtcc ccggggcagg cggctgctcc agttgctgga tgcggatccg ctcatcacta
     4921 aagatgcggc catcgcctac acgatgaata actatgacct tcttgcggtg ccctggcaga
     4981 ccgctcttgc cgcggcagtc gcggcacggg gtgaagacaa tccgccggaa gaggaacttg
     5041 cccgagcggt gcagggcatt ctgagatggg atggctactt cagcaaagag gagcctttta
     5101 cttttctcta taaacggtgg cgcctgaagt gtcaggatgc cattgatatc gctgccattg
     5161 cggatggaga gccgctgggt gcggaggatc aggaaaagat gctgtctctc ctggcggagg
     5221 cgcagcagga aatgaaggag aagtacggca gcttcgaggt gccctggggt gatctggtca
     5281 agatcggaag aggcggacgc tatttccccc ttgaatgcgc tgatttcggc ggcggaaaag
     5341 acaagaaaaa tcagaccgaa accgttctgg atgtggcttc aaaagaaatg ccggacagcg
     5401 acggaaaact ggtcggtcat aacgggtccg gttcactgat gatagctttt ctccacaagg
     5461 acgggattga atcgcacagc ctcgttgcct ggggtcagag tgcggatccg gaatcaccgc
     5521 attacgtgga tcagtcggaa aagctctatt ccccccgcaa actgaagccg acctggttta
     5581 caaaagaaga attgatggag catctggaat cgaccagaac gatcacggtt cccgggcgat
     5641 agcatcagcc tgcgtctgtt tctgccggga aaacttcggc gagaaagagg tcgactacgg
     5701 aagtgaattc atccgtatag cggctcagaa aattccagtg tccagcgcca tgaaagaagt
     5761 gaagtttctt caaggcggcg gggcagcggt cgtagagcga ctgcgtctca tggacaggga
     5821 tttctatttc cgcatcgccc gccagaatca gtgtgggcat gacagcatac ttcatgaaat
     5881 caacgggacg catggcaaaa ggattggcgc ctgtgagaag ggcaaagccg gcgtaaaacg
     5941 ggtaggcgat gaaatgaggc gtgtaaaata ctgccagcct gtttgccacg gcactggtca
     6001 gtgtatcgta acttgatt
//
LOCUS       DAOPWB010000169         5770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_13855_length_5770_cov_5.193526, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000169 DAOPWB010000000
VERSION     DAOPWB010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13855_length_5770_cov_5.193526"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            955..1620
                     /locus_tag="PKN92_11930"
     CDS             955..1620
                     /locus_tag="PKN92_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNZ49542.1"
                     /translation="MKKLLIVAIAVAMMTLGVPAEAAKVWDDMSWWGNTGATPEPHIE
                     TGRCPAQMARSGYWWWPKEPASNVDDSELWGNRGIVYRNWVKPEAEVTPPPPPPPAPP
                     APAQRTAIILNNVLFDFDKAVLKPEGKAEVDKLVAEMKKYPEDKVVIEGHTCDIGNHA
                     YNMGLGQRRADAVKKYMIDSGIDAARIETQSFSFDRPAVPNDSAANRKLNRRAEFKIT
                     LGS"
     gene            1707..2606
                     /gene="xerD"
                     /locus_tag="PKN92_11935"
     CDS             1707..2606
                     /gene="xerD"
                     /locus_tag="PKN92_11935"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNZ49543.1"
                     /translation="MSAPSVPLLENMERFLQAAVFEAGLSEKTLEAYASDLHRYCERM
                     MGQEIVRAEDILFEDVLDHLGMLQAEGLSARSLARHLSAIRRFHQFLAAESLCPSDVT
                     AICDTPQMMRRLPRCLSAAEVEALLNAPLADTEEGIRDQALLEIFYSCGLRISEVIAL
                     PVKAVDLEESAVRVSGKGGKARLIPLGRDAMEKIRRWLPVRTLWALRDTALFIGKSGR
                     RLGRTTAWTLIKKYATAAGIQQNVTPHMLRHSFATHLLDNGADLRAVQEMLGHADIGT
                     TQIYTHVSTERLTQAHKKFHPRA"
     gene            2645..5200
                     /locus_tag="PKN92_11940"
     CDS             2645..5200
                     /locus_tag="PKN92_11940"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNZ49544.1"
                     /translation="MPDGEPILKRMTKETAAALCAHIRDAGGCEVFFAGTADAGGMLT
                     AVRVLARGNANAVPALFAQLNLREVVLHNHPSGDLTPSDADLDLASIYSAHGHGVYII
                     DNEATRLYVVVEPFLPKAAVKLTSRELSAAFSSDSPLARELPGFESRPQQIDMAAAIG
                     KAFNENGIAIIEAPTGVGKTMSYLLPAIYWSLRNKERIVVSTRTINLQEQIMYQDLPL
                     LKRCLKEDFHACLVKGRGNYLCLQKMERTLSEITLFEDEESAGQLRAIAEWADETEEG
                     STSDLPFVPSWFVWERVNCESDVCTGSACPYFRECFFMNARKEMARADLLVTNHHMFF
                     ADVAVKKEKGDFLAEAVLPRYSRIILDEAHSIEDAATEYFGLSATLAGMRKTMNKLLR
                     IEFEQQRGLLPFLKLKLMRNCPQVTTEEYNAYIELIDKGLVPALLLCQDALEEAFYSL
                     REYTAEKSGQIGREIKWRLTPDILAGPQLRMIHDEVVVPAVNQTRTVIRLGQELYEKL
                     SSILPLKAGEESPVQLETAELFAYIRRLQGVAEALIECTDESLADNTVRWIEIDGRDE
                     NKIRVVRCPLSVGEPLSEWVYTHMDTIVFTSATLAVEQKFRYFMERVGLDLMPEKRII
                     TELLDTPFDYSSQCLLCVPCDGPDPKDSRYVEETTEQIRKILKITRGHAFVLFTSFYA
                     LDYTFRQMEEELKDLGITPLCQGKATRSQLLEVFRRDTSSVLFATDSFWEGVDVPGEA
                     LQCVIVTKLPFRVPSEPVLEARAEAIAQAGGNSFMQYSVPQAVIKLRQGFGRLIRRRS
                     DWGTVVVLDNRLIRKYYGKAFLKSLPHVPVVQENAEKLWSYLSDFYEERRSNS"
     gene            complement(5241..>5770)
                     /locus_tag="PKN92_11945"
     CDS             complement(5241..>5770)
                     /locus_tag="PKN92_11945"
                     /inference="COORDINATES: protein motif:HMM:NF025097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HNZ49545.1"
                     /translation="FRETVTVSSSTVYAGDTEEKSTRIGNNCLFMACVHIAHDCTVGN
                     QVIMANNSALAGHVEIHDGAILGGLVGIHQFCRIGTMAFIGGMARVNMDALPFMITEG
                     HPARCYGPNVVGLNRAGLSKDSVSLIRRMFKLLYRSGLNTSQAVQEIVQSIEDCHEKT
                     VLLDFITGSGRGIVH"
BASE COUNT         1310 a         1459 c         1614 g         1387 t
ORIGIN      
        1 tcctgactct atatctccgg ttgtgccagc ttcaaagatg cgaaccccca tgtctaccag
       61 ctcgacaggg cgggtgtcgt caaagcgtat cattgcccac cctatggaat tggttcccag
      121 atcaatgccc agtacataag gagcgtgctg atttgtcatg atatatgtcc tttttatttt
      181 tccgccttaa ggcgaatttt tgttttcaga tactttcgtt tgttttagaa tcactgctga
      241 tggttgcatt tgacttttgg ttgcgcatgc gttatactta acatattagt atacctgagc
      301 ggtgatttca ttactgatta tagtaaggat tcatcacggt gggctccgtt ggtaacaacg
      361 tccctgctgc cgcctcgaaa gaggcggttt ttttattact cgttatcaat ctttttcccg
      421 ccaacatact ctcccgccca attgtcggga atttatctgt tattctactc tatagaacga
      481 attaaagcaa gaggtctgtt ctatattttc gcaatacaat atttcgacag ccatcgtatg
      541 tgccgtgttg tgactgcttg ctgctggccc caaaattaat tgatgcggat cgatcttaat
      601 tcgcttcgat ttccgattgt gctgtacgct gttcagcggc ggttttgtgt aaaaagggca
      661 ggggagagca ggtgaaaaaa tggcggagcc gaaaagggga aaaaggggat aacggcaact
      721 ttaggaatgt tgacttgcga ttcttctgtc aatatgctac aatacggcgt gaaagttcct
      781 gatcaatggg gacggaagta attgcgctcg ctgaccgttt ccaagattga cattcgggaa
      841 tgaaatctgg tttaatgtag ttagaaagta tgtgcgttcg ttgttgtatc tgtcaagagt
      901 ttttgtggaa gggcgcttac ctaagaacct ttagcgagaa gaaggagaag caaaatgaag
      961 aaactgttaa tcgtcgccat tgcagtggca atgatgaccc ttggcgtgcc cgcagaggca
     1021 gccaaagtat gggacgacat gtcctggtgg ggcaacacgg gcgcaacccc tgagccgcac
     1081 attgaaacag gtcgctgccc ggcgcagatg gcccgttccg gatactggtg gtggccgaaa
     1141 gagcccgcca gcaatgtgga tgactccgag ctgtggggca accgcggtat cgtctatcgc
     1201 aactgggtaa aacccgaagc tgaagtgact cctccgcccc cgccgccgcc ggctccgcct
     1261 gctccggcac agcgtaccgc catcatcctg aacaacgtgc tctttgactt cgacaaagcc
     1321 gttctgaaac ccgaaggcaa agccgaagtt gacaaactcg ttgccgaaat gaagaaatat
     1381 cccgaagaca aggtcgtcat cgaagggcat acctgtgaca tcggtaacca tgcctataac
     1441 atgggtcttg gtcagcgccg tgccgacgcc gtcaagaagt atatgatcga cagcggtatt
     1501 gatgccgccc gcatcgagac tcagagcttc agcttcgacc gtccggcagt cccgaacgac
     1561 tccgcagcga accgcaagct taaccgtcgc gctgagttca aaatcacgct gggcagctga
     1621 tacggccgcg tagattaacc ggtttatttt caaaccccgg tgtccgtatg tgacatcggg
     1681 gtttttttaa atggaggcat tcgcctatgt cggcaccgtc tgtgcccttg ctggaaaata
     1741 tggagcgttt tctgcaggct gctgtctttg aggcggggct ttcggaaaag acgcttgaag
     1801 cctacgcctc cgatctgcat cgctactgtg agcggatgat gggacaggaa attgtccggg
     1861 cagaagacat cctttttgaa gacgtgctgg atcatctcgg gatgctgcag gcagaagggc
     1921 tttccgcccg gtcactggcg cgccatctca gtgccattcg tcgctttcat cagtttcttg
     1981 cagccgaatc gctctgtccg tccgatgtaa cggcgatttg tgacacgcct cagatgatgc
     2041 gacgcctgcc gcgttgtctc tctgctgccg aggtggaggc gctgcttaat gcgcctttgg
     2101 cggatacgga agaagggatt cgggatcagg cgcttcttga aattttttat tcatgcggtc
     2161 tgcgtatttc cgaagtgatc gcgctgcccg taaaagccgt tgatctcgaa gaatccgctg
     2221 tccgtgtttc cggaaaagga ggcaaagccc ggctgatccc gctgggcaga gatgccatgg
     2281 aaaaaatacg gcgctggctg cctgttcgta ctttatgggc actgcgtgac actgcgcttt
     2341 tcatagggaa aagcggcaga cggctgggca gaaccactgc ctggacgctg attaaaaaat
     2401 atgcgacggc ggccggaatt cagcagaatg taacgccgca tatgctgcgc cattcttttg
     2461 ccacccatct gcttgacaac ggtgcggatt tgcgggcggt gcaggaaatg ctgggccacg
     2521 cggatatagg cacgacgcag atctataccc atgtcagtac cgagcggctg actcaggcgc
     2581 acaagaaatt tcatccccgt gcctgataag ccgggcgatg gcgcaaaacg agtgagatgt
     2641 ttttatgccc gacggcgaac ccatactgaa gcgaatgaca aaagagactg ccgcagccct
     2701 ttgcgcacat atccgtgacg ctggcgggtg tgaagtcttc tttgccggaa cggcggatgc
     2761 aggcggcatg ctgacagcgg tgagagtact tgcccgggga aatgccaatg cggtgcctgc
     2821 tctctttgcc cagctgaacc tgcgcgaggt ggtgctccat aaccatcctt ccggcgacct
     2881 gacgcccagt gatgccgatc ttgatctggc atccatttac agcgcccacg ggcatggtgt
     2941 ttatatcatc gacaatgaag caacgcgtct ttatgtggtt gttgagccct ttctgccgaa
     3001 agcggccgtt aaactcacga gccgtgaatt gtccgctgcc ttttcttcgg acagtccgct
     3061 tgcccgggag ctgccgggct ttgagtcccg tccgcagcag atcgatatgg cagccgccat
     3121 tggaaaagcc tttaatgaaa acggcattgc cattatcgaa gcgcctaccg gtgtggggaa
     3181 gacgatgtcc tatcttctcc cggcgatcta ctggtctttg cgcaataaag aacggatcgt
     3241 cgtttccacc cgcaccatca atctgcagga acagatcatg tatcaggatc tgccgctgct
     3301 gaaacgctgt ctcaaagaag actttcatgc ctgcctggtg aaaggcaggg gcaactacct
     3361 ctgtctgcag aagatggagc gtaccctctc cgaaatcact ctttttgaag atgaagaaag
     3421 tgcggggcag ctgcgcgcca ttgccgagtg ggcagatgaa accgaagaag gcagcaccag
     3481 cgatttgcct tttgtgcctt cctggtttgt ctgggaacgg gtgaactgtg aatccgacgt
     3541 atgcacgggc tctgcctgtc cttacttccg cgaatgcttt ttcatgaatg cccgcaagga
     3601 aatggcacgg gcggatctgc ttgtgaccaa tcaccatatg ttttttgccg atgttgccgt
     3661 gaaaaaggag aagggcgatt ttcttgcgga agcggtgctg ccccggtaca gccgtatcat
     3721 tcttgatgaa gcgcacagta ttgaagatgc ggcaacggag tatttcggac tttccgccac
     3781 actggcaggc atgcgaaaaa ccatgaataa gctgctgcgc attgaattcg agcagcagcg
     3841 gggactgctt ccattcctca agctgaagct gatgcgcaac tgccctcagg tgacaacgga
     3901 ggaatacaac gcatatattg agctcatcga caaagggttg gttccggcgc tgctgctgtg
     3961 tcaggacgcg ctggaagagg ctttttattc gcttcgtgaa tacaccgctg aaaaatcggg
     4021 acagatcggc agagagatca aatggcggct cactccggat atccttgccg ggccccagtt
     4081 gcgcatgatt cacgatgagg ttgttgtgcc ggcggtaaat cagacccgca ccgtgatccg
     4141 gttgggtcag gagctgtatg aaaaactgag ttccattctg ccgctgaagg ccggggaaga
     4201 atcgccggtt cagctggaga cagcggagct tttcgcttat atccggcggc tgcagggcgt
     4261 ggcggaagca ctcattgaat gtacggatga atcgcttgcg gacaatacgg tgcgctggat
     4321 agaaatagac ggcagggacg agaataaaat ccgggtcgtc cgctgtccgc tgtctgtcgg
     4381 cgagcccctt tccgaatggg tctataccca tatggatacc attgttttta cgtcggcgac
     4441 gctggcggtg gagcagaagt ttcgctattt tatggagcgg gtagggctgg atctgatgcc
     4501 cgaaaagcgg atcattaccg agctgctgga tacgcctttc gattacagca gtcaatgcct
     4561 tctgtgcgtg ccttgcgacg gccccgatcc taaagacagc cggtatgttg aagaaacaac
     4621 agagcagatc cggaaaattc tgaaaataac ccggggtcac gcttttgtcc tgttcacgtc
     4681 cttctatgcg ctggattata cgttcaggca gatggaagag gagctgaaag acctggggat
     4741 taccccgctg tgtcagggga aagccacccg ctcccagctt ctggaagtat ttcgacgcga
     4801 tacatcgagc gtgctttttg caaccgacag tttctgggaa ggtgtggatg tgcccggaga
     4861 agcgctgcag tgtgtgattg tgacgaagct gcccttccgt gttccttccg aacctgttct
     4921 ggaggcacgt gccgaagcca ttgcgcaggc aggcggcaat tcttttatgc agtattcggt
     4981 accgcaggcg gtgatcaaac tgcgccaggg attcggcaga ctgatccggc gccgcagcga
     5041 ttggggaact gtggttgtgc tggacaaccg ccttatcaga aagtactacg gaaaggcttt
     5101 cctgaaatca ttgccgcatg tgccggtagt tcaggagaat gcggaaaaat tgtggagtta
     5161 tctgtccgat ttttatgagg aacggcgcag caattcctga ggcggaaacg ggaaaaggac
     5221 agaatctttc ctggaatact tcagtggaca atgccgcggc cggatccggt aatgaaatcc
     5281 agcaacacgg ttttttcgtg gcaatcctct atggactgaa caatttcctg cactgcctgg
     5341 cttgtgttca atccggaacg gtacagcagt ttgaacatgc gtcggatcag tgaaacggag
     5401 tctttggaca gacctgcccg attcagcccc actacattcg gaccgtagca ccgtgcagga
     5461 tgcccctcgg tgatcataaa aggcagtgca tccatgttga cccgtgccat gccgccgata
     5521 aaggccatgg tgccgatgcg gcagaactgg tgaatgccca ccagcccgcc cagaatggcg
     5581 ccgtcatgaa tctccacatg ccccgccaat gcactgttat tcgccatgat cacctgattg
     5641 ccgaccgtgc agtcgtgggc gatgtgcacg catgccataa aaaggcagtt gttgccgatg
     5701 cgggtggatt tttcttccgt gtcccctgca tagaccgtgc tggaactgac ggtaaccgtt
     5761 tcccggaatg
//
LOCUS       DAOPWB010000170         5719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_14028_length_5719_cov_3.816384, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000170 DAOPWB010000000
VERSION     DAOPWB010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14028_length_5719_cov_3.816384"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2688
                     /locus_tag="PKN92_11950"
     CDS             <1..2688
                     /locus_tag="PKN92_11950"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNZ49546.1"
                     /translation="IFNNGSAPVLSFLTIVNNSTDDSYSYNGFGVGSGALYTWNADTV
                     LTNSIVYGNSSGLLHQAEAGNLTALAVTFSDIEGGFAGAGNIDVDPLLGNGDYTALLG
                     ASIGGYVPALGSPVVNAADPNVGGDDLLGVPRPIGAAADMGAFELSVQGPAIDCIASA
                     PIDFITTPQLTDPFVLVDLDNAFFEAPYWKLTMENKVFGCADIPLSDIGLTAYDILGR
                     AATCRADVPVTESEAPVAVCNALTVELDSSGAYTLTAADLQVLSAGSSDNCTAAANLT
                     VTASPMAFDCDDAGKTVNVAITVEDSEGNTDTCTAQVTVEDNIAPTAATPANSINVEL
                     NALGNYTLSEADVQALAANATDNCAVNFALSTVDQMPFKCSDIGANTVTVTWHDFHGN
                     TVTGPATVNVADVGLPTLIGLVDRQYSIATGDFTLAQALAGVSATDVCDGDLTNAIVV
                     EVFDADDNPVPFPVPTSWPLDPSETGYVFTMKYTVADNSGNEASGEVGLVFNAVKLPE
                     ITVLGDNPIYHECHTVYFYDDDPGATAWDPQGLVDITGLITTTFTVDQNVPGTYQVIY
                     SVPVPGYPSLAPVVATRDVIVIDTIKPTITLSGSTTVISPLGEAYHEPGYTAFDSCAG
                     SLSNVVVVTGAVDINTPGAYVLDYNVTDSEGNVADTKTRTVVVAEEVSFTRHPSNARK
                     YSTDAPFNLEANYTGGFDLDGHEWFRDGVGLGLQAPETGGNTVTLTVNPASHPLGTVP
                     YNLVVTDYGIFTYSSNNAFVEVNLPLKVDQGLADLAVQAGEEADWSIAVSGGLGNVTY
                     QWYASLNGGKAFAPVVDGPLGDGAYAGATSDTLQLVPFDPEMVGQYQVQVSDDFSTIT
                     VGPANLILDYGIPAAGAIGIAVLALATALGGARALRKRD"
     gene            2863..3729
                     /locus_tag="PKN92_11955"
     CDS             2863..3729
                     /locus_tag="PKN92_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49547.1"
                     /translation="MRFGVLFLFPLLVFAFASAAESDRQEHSASVTAKEYPHQIQSAL
                     DFLEREARGQEKSWQVYAFLDFLQRRFDLPEKYKFINLCQGVIWSEDDEQLASFFGKL
                     INPDHQIDCAAIPSVRANSLNHLMGHALACDECPLPENYFQLLQEGFDAELKAERPGY
                     ITTHLLLCRLWLEELQCPCEKLNGDDKAQFVQLLLKIVEQNKGATDLAFEAMAFLFYI
                     GEGEEIKQEWLENLGKVQTRTGGWGYQPGLKPEGHPTMLALWVLLEKEFPDVTPGVRM
                     LRPSKKFLVVDE"
     gene            3860..4756
                     /locus_tag="PKN92_11960"
     CDS             3860..4756
                     /locus_tag="PKN92_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49548.1"
                     /translation="MVNTRDYSNKSWISRFFSRVFGVFGKTKPPEREHLVDLRGKVVL
                     LDNDELLLVWEKRLRNIRLQKEEEAWNRYEAQYGAGSPMVTQAFVRWQEDLYRFIEKE
                     KDRFLRVRQGCVPALDKLVQQGARTIVVTKGAKPYTQKCFFLTGLSSSITDIYSPPPG
                     SRQKQFAEAVRDHGIDSLEKCLKNAIVVGHDLDKDMAWELVPPKGPGADGCAPVFVLL
                     DTMTFEKDVLAPLDAMTEVIELLALKGKSDIWRGFNVLCQSAQTTTPNYQFKVGLYDH
                     PRLQGKARVPVVYDVRRIGISY"
     gene            complement(4845..>5719)
                     /locus_tag="PKN92_11965"
     CDS             complement(4845..>5719)
                     /locus_tag="PKN92_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNZ49549.1"
                     /translation="YGGAKEKRSISASYYGWDDTFALVRELQPNAVIFSHLGDVRWVG
                     NESGFAGDPCWATFTPQIEEDAKALNVGIRNGTHWQPAEVDVSIRPGWFYHNREDAKV
                     RTPGNLMNLYFQSVGRGASLLLNLPPDRRGRIHERDAQSLIAFGKRLKAMYGTNLAAG
                     ASASASNVRGGDKRFSAACVLDGKSDSYWCSDDEVLTPELVLELPEARTFDVVSLREY
                     LPLGQRVDRWALDARVDGAWKEFACGTAIGSRRLWRGNAVTTDSVRLRIMEAAACPAI
                     SEVALHHLSGKSIR"
BASE COUNT         1283 a         1595 c         1518 g         1323 t
ORIGIN      
        1 atctttaaca atggctctgc tcccgtgctt agcttcctga ccatcgtgaa taacagcacg
       61 gacgacagct acagctacaa cggcttcggc gtcggcagtg gtgctcttta cacctggaat
      121 gcagacacag tgttgaccaa ctccattgtg tatggaaact ccagtggtct cctccatcag
      181 gctgaagccg ggaatcttac cgctctggca gtcaccttct cggatattga gggcggtttt
      241 gccggtgctg gcaacattga tgttgatccg ttgcttggca atggcgatta tacggcgctg
      301 ctgggcgctt ccatcggtgg ctatgtgcct gccctgggat cgcctgtcgt gaacgccgct
      361 gatcccaatg taggcggcga cgacctgctc ggcgtgccga gacctatcgg tgctgctgcc
      421 gacatgggtg cctttgaact cagcgttcag gggcctgcca ttgactgcat cgcttccgcg
      481 cccatcgatt tcattacgac tcctcaactg accgatccct ttgtgttggt agacctcgac
      541 aatgccttct tcgaagcgcc ctactggaag cttacgatgg aaaataaagt cttcggttgt
      601 gccgacattc ccctgtccga tatcggtctg acagcctatg acattctggg tcgcgctgcc
      661 acgtgccgtg ccgatgtgcc cgtcactgaa agcgaagctc ctgttgcggt gtgcaatgcc
      721 cttactgttg aacttgacag cagcggtgca tacaccttga cggcggctga tctccaggtg
      781 ctcagcgcag gcagttccga caattgcaca gcggccgcta atctcactgt aacagcctct
      841 cccatggctt ttgactgcga tgatgccggt aagacggtca acgttgccat tactgtggaa
      901 gacagcgaag gcaacaccga cacatgcacg gctcaggtca ccgttgagga caacattgct
      961 ccgacagcgg caacgccggc aaattccatt aatgtcgaac ttaatgcgtt gggcaattac
     1021 accctcagtg aggcggacgt tcaggccctg gctgcaaatg ctaccgataa ctgcgctgtc
     1081 aacttcgccc tgtccaccgt tgaccagatg ccgtttaagt gcagtgatat cggcgccaac
     1141 accgttactg ttacctggca tgacttccac ggcaatacag taaccggtcc tgcaactgtt
     1201 aatgtcgccg atgtgggtct tcccacgctc atcggtcttg tggatcgcca gtacagcatt
     1261 gccacaggtg atttcacgct ggcacaggcg ctggcaggcg tgagtgccac cgacgtgtgc
     1321 gatggcgatc tgacaaatgc cattgttgtc gaggtcttcg atgccgacga caatccggtt
     1381 cccttcccgg taccgacctc ctggccgctt gacccgagcg agacaggtta cgtcttcaca
     1441 atgaagtaca ccgttgctga taacagcggc aacgaagcgt ccggtgaagt cggcctggta
     1501 ttcaacgctg ttaagcttcc tgagatcacg gtgcttggtg acaatcccat ttaccatgaa
     1561 tgccacactg tctacttcta cgacgacgat ccgggcgcaa cggcttggga tccgcagggg
     1621 ttggttgaca tcactggtct gattactacg accttcactg tcgatcagaa cgttcccggc
     1681 acctatcagg tgatctattc cgtgccggtg cccggctatc ccagcctggc gcctgtggtc
     1741 gccacccgtg acgtgatcgt gattgacaca atcaagccga ccattacgct ttcaggcagc
     1801 accacggtga tttcgcccct tggcgaagcc taccacgaac ccggctacac ggcgtttgac
     1861 agctgcgcag gctccctgag caatgtggta gtcgtgaccg gtgctgtgga catcaacacc
     1921 cccggtgcgt atgtccttga ctataacgtg accgattctg aaggcaacgt tgccgatacc
     1981 aagacacgta ctgtagtggt tgccgaggaa gtttccttca cacggcatcc ctccaatgcc
     2041 cgcaagtact ccaccgatgc gcccttcaat cttgaagcca actacacagg cggctttgat
     2101 ctcgatggtc acgagtggtt ccgcgatggc gtcggtcttg gattgcaggc tcccgaaaca
     2161 ggcggcaata ctgtcacgct gacggtcaat cctgccagtc acccgctggg aaccgtgccg
     2221 tacaatctgg tcgtaaccga ctatggtatc ttcacctaca gcagcaacaa tgcctttgtt
     2281 gaggtcaacc ttccgctgaa agtcgatcag ggtcttgccg accttgcggt acaggcaggt
     2341 gaagaagccg actggagcat cgctgtgtcc ggcggtctcg gcaatgtcac ctaccagtgg
     2401 tatgccagcc tcaacggcgg caaggccttt gctcctgttg tggacggacc tcttggcgac
     2461 ggtgcttacg ccggtgctac gagcgacacc ctgcagttgg tgccctttga tcctgaaatg
     2521 gtcggtcagt accaggtaca ggtcagcgat gatttcagta ccatcacggt cggacctgcc
     2581 aatctgattc tggactacgg cattcctgca gccggtgcca tcggcatcgc agtgcttgcc
     2641 cttgccacag ccctgggcgg tgcccgggcg ctgcgtaaac gcgactaaaa agagggcgta
     2701 atgccctgat accgagatga gtcagccgcc ggttccggga ggagccggcg gctttgtctt
     2761 cactcaactt ggcgtaaagc aaaagggcga tgcgtgcttc gatgccgaaa gtcaaaaagt
     2821 aaaaacactg ttccttttta aggaagagaa gaaagaattc ctatgcgttt cggtgtccta
     2881 tttttatttc cgctgctcgt ttttgcattc gcttccgcag ccgaaagtga ccggcaggaa
     2941 cacagtgcgt ctgtcactgc caaagaatac ccccaccaga ttcagtctgc cctggacttt
     3001 ctggaaagag aggcaagggg tcaggaaaaa agctggcagg tctatgcctt ccttgatttt
     3061 ttacaaagac gttttgatct gccggaaaaa tacaaattca taaatctttg ccaaggcgtc
     3121 atctggagtg aagatgacga gcaattggcg tcttttttcg gaaaacttat taaccccgat
     3181 catcagattg actgcgctgc tattccgtct gtccgggcta acagcctgaa tcatcttatg
     3241 ggacacgctc ttgcctgtga cgagtgcccg ctccctgaaa actactttca gctgctgcaa
     3301 gaaggctttg acgccgaact aaaggctgaa cggcccgggt acatcacaac gcatctgctc
     3361 ctttgcagac tctggcttga agaactccag tgtccatgtg aaaaactgaa tggtgacgat
     3421 aaagcgcagt tcgttcagct gcttttaaaa atagtagaac agaacaaagg ggcaacagac
     3481 cttgcctttg aagcgatggc gtttcttttt tacataggtg aaggggaaga aattaaacag
     3541 gagtggctgg agaatctcgg aaaagtacag accaggacgg gaggctgggg ctatcagccc
     3601 gggttaaaac ccgaaggaca cccgaccatg ctcgctctgt gggtgttgtt ggaaaaggaa
     3661 tttcctgatg ttacgccagg cgtgcgcatg ctgcgccctt caaagaagtt tcttgtggta
     3721 gacgaataag cgggccggaa aagaagtgat acaacgcaga gaattttaat ttggcttttt
     3781 ctccttgatt ttgtaaaata aaagtgtctg atcatgtaaa aaggctgggt catttcactg
     3841 gacttatgga gtggatactg tggtcaacac acgcgattac tcgaataaaa gctggatcag
     3901 tcgttttttt tcccgcgttt tcggtgtttt cggaaagact aaaccaccgg aaagagaaca
     3961 ccttgttgac ttgcgcggca aggtcgttct tctggacaat gacgaattgc ttcttgtttg
     4021 ggagaagcgt ctcagaaaca taaggctgca aaaagaggaa gaggcgtgga atcggtatga
     4081 ggcacaatac ggcgcaggca gccccatggt cacacaggct ttcgtccgat ggcaggaaga
     4141 tctttaccga tttattgaaa aagaaaaaga ccgctttctt agggtgcggc agggttgtgt
     4201 tccggcgctc gataagcttg ttcagcaggg tgcgcgcacg attgtcgtaa ccaaaggtgc
     4261 caagccctat acccaaaagt gttttttcct gactggcctt tcctcttcca taaccgatat
     4321 ctactctccg ccgccgggat cccgccaaaa gcaatttgcc gaagccgtcc gcgatcacgg
     4381 gatagattct ctggagaaat gcctgaaaaa tgccatcgtt gtcgggcacg atctggataa
     4441 agatatggct tgggaactgg taccccccaa agggcccggt gccgatggct gtgcgccggt
     4501 tttcgtgctc ctcgatacga tgacatttga aaaagatgtg cttgctcccc ttgatgccat
     4561 gactgaagtg attgaactgc tcgccctcaa aggaaaaagc gatatctggc gtggcttcaa
     4621 tgtgctgtgt caatcggctc aaaccaccac gcccaattat cagtttaaag taggactcta
     4681 cgaccatcca cgcctgcagg gaaaagcccg ggtgcctgtc gtctacgatg tgcgccgcat
     4741 cggcatctcc tattagcagc gcgccttctt catacgcaaa ctgataaagg gcgcagccgt
     4801 ctccccggga ccatgtggcg tgcagtatcc ggcagctccc tgcatcagcg tatgctttta
     4861 ccggacaagt gatgcagagc cacttcgctg atcgccggac aggcggctgc ttccatgatg
     4921 cgaagccgta ccgaatcggt agttacggca ttgccgcgcc agagcctcct gctgccaata
     4981 gccgtgccgc aagcaaactc cttccaggca ccgtcaacgc gtgcatccaa agcccagcgg
     5041 tccacacgct gccccaaagg caggtactcc cgaaggctga ccacatcaaa ggtgcgggct
     5101 tccggcagct ccagcaccag ctccggcgtc agcacctcat catcgctgca ccagtagctg
     5161 tcgcttttcc cgtcaaggac gcatgccgct gaaaaacgct tgtccccgcc ccggacattg
     5221 ctcgccgaag cggatgctcc ggcggcgagg ttcgtcccat acatggcttt cagccgcttg
     5281 ccgaaagcaa tcagggactg cgcatccctt tcgtggattc tgccacgccg gtccggaggc
     5341 agattcagga gcagggacgc accgcgcccc accgactgaa aataaagatt catcaggttg
     5401 ccgggggtac gcactttcgc gtcttcccga ttgtggtaaa accagccggg ccggatcgat
     5461 acatccactt cagccggctg ccagtgggtg ccgttgcgta tgccgacatt taatgctttg
     5521 gcatcttcct cgatctgtgg tgtaaaagta gcccagcagg gatcacccgc aaagcctgat
     5581 tcattgccta cccaccgcac atcgccaagg tgactgaaga taaccgcatt gggctgaagc
     5641 tcccgtacca gggcaaaagt atcgtcccag ccgtaatagg atgcggaaat agaacgtttt
     5701 tctttggcac cgccgtacc
//
LOCUS       DAOPWB010000171         5697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_14096_length_5697_cov_4.666430, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000171 DAOPWB010000000
VERSION     DAOPWB010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14096_length_5697_cov_4.666430"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKN92_11970"
     CDS             complement(<1..528)
                     /locus_tag="PKN92_11970"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNZ49550.1"
                     /translation="MSVILSGLVVAAGIFGAAFESEYIFPLQDKHVHASSIVETPDGD
                     LLAAWFHGSGERGADDVQVQGARLRKGDSQWSPVFIMADTPGFPDCNPVLFIDAKERL
                     WLFWIAVLANEWENSLLKYKRADNFSADGAPEWNWQDVIIFKRVFQFIGKRVARFKDN
                     NILPVPFRGTVSTEVV"
     gene            complement(582..1106)
                     /locus_tag="PKN92_11975"
     CDS             complement(582..1106)
                     /locus_tag="PKN92_11975"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNZ49551.1"
                     /translation="MDLSEVIRNVPNFPKPGVQFKDITTLVLNPEAFLQVIDVFANRY
                     AADKPDAILAVESRGFVFGGALAVRLGVPLIMARKPGKLPADTIRESFNLEYGTDAIE
                     IHTGALLPGWRVLILDDLLATGGTVSAACSLVKRAGGTVQEAAFVIELLPLGGREALS
                     PVPVFSQITFHVDE"
     gene            1305..4610
                     /gene="mfd"
                     /locus_tag="PKN92_11980"
     CDS             1305..4610
                     /gene="mfd"
                     /locus_tag="PKN92_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNZ49552.1"
                     /translation="MTQLPEFSITATLKNAFDKKPKGAVDLFGVLGAAKALVPLQLAR
                     KMEHALLLIASSRNEAEALYEDLASSAGEEACLHFPAWEVSPDEIMDPAGDIIAERIH
                     TLERMAEMSERKNPFYVVASVQAFLQYVTSLKSLQDKILHVRTGEILPMETLIDHLLQ
                     RGYTREVMVEQRGDFSVRGGILDVFPMSAELPCRLEYFDDEIESIRAFEPETQRSVTR
                     LETVKIPPCSEKDALKEAHHLKSLVPFSACFPPDTLVATVESLAVRDKAVALTEQWSQ
                     SPFFMTWQQAQAHLAKFPRLTLSQMPEQRQEASSRFAMQMSMVENFSGRTNEFWEQIH
                     AWDMQDYRVHILCVNSGEQRRFIELLHEHGYRPERDSRITVSLGRIHAGFVSHADKMV
                     LLSEKEVFGRRYVRRKRRRFEAGAAITEYADLRAGDYIVHEVHGIGRYQGLRRLEGRS
                     VDYLTLRYAGGDTIYVPVTQLDQIQKYMGGDGVLPKMDRLGGATWSATKARVRRAVRE
                     MTAELVRLYAARESSEGYAFTRDTLWQTEFEDAFEYEETPDQRRAIDEVKRDMESDRP
                     MDRLLCGDVGYGKTEVALRAAFKAVMDKKQVAMLAPTTVLVQQHYNTFRERFAAYPIT
                     VEVLNRFQSGRQIRSTLERLKSGEVDVVVGTHRLLSKDVGFRDLGLVIIDEEQRFGVR
                     HKERLKEMRTHVDVLTMSATPIPRTLHFSLLGIRDMSVINTAPNDRLPIHTCIDAWDK
                     ELIREAITRELARDGQVFFLHNRVQTIDRVAAFLKQIVPHARVGIGHGQMSKTELESV
                     MTGFINHETDVLLCTTIIASGIDIPNANTIIVDRADQFGLSQLYQIRGRVGRYKHRAF
                     AYMLIPGDRSLTSEAQARLQALHDFSALGSGYRIAMRDMEIRGTGDLLGADQSGHIAT
                     VGYETYREMITEAVAEAQGKSLYKRSLPSFDLAVDAFIPDAYMPIASQKIILYRRIAA
                     LKTLEEVQELQEELRDRFGPVPRPLRRLLQIMEARARAADLGIAALEAGQRAATVYFE
                     TGRTLAQGVQAQLRRMYGDTIVFSWKDKPAMSLTYDNIEQGLENFSLLLRQLHAMMTE
                     DSMEEEDPLLYGL"
     gene            complement(4593..>5697)
                     /locus_tag="PKN92_11985"
     CDS             complement(4593..>5697)
                     /locus_tag="PKN92_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNZ49553.1"
                     /translation="ATGILAPISSTLALLPGHALLPLVSLIQWVNRAALLLPVSFLYV
                     NRPTLCALGFYCLFLIFFYLVLYEERKHRRNRLLCILCVCACLVFWRPWTAPALVDFL
                     DVGRGDSSFVRTPGGTSLLIDAGDSDGYTDAGKRMIVPYLRANRISHLDYLVISHADR
                     DHIGGAFYVLNHFPVKKVVLGAVRDDYNALESNLIALCAQKNIPVLHLSRGDFLPAEK
                     ASVEVLHPPLHNTDNLSRNDGSLVLAVAFDGIRILFAADIEEAGEKEIQPCIQTPFDV
                     LKVAHHGSPTSSRIEFLDAVRPSLAVVSAHRAGSRGTLMTPAMAERFEARKITLLRTD
                     WHGAIRLQADRKNGGYIVSTARESRGYSLQPVE"
BASE COUNT         1380 a         1476 c         1563 g         1278 t
ORIGIN      
        1 gacaacttca gtgctgacgg tgccccggaa tggaactggc aggatgttat tatccttaaa
       61 ccgggcgact ctttttccga tgaattgaaa gacgcgttta aagataataa catcctgcca
      121 gttccattcc ggggcaccgt cagcactgaa gttgtccgcc cgcttatatt tgagcaggct
      181 attttcccat tcattggcaa gaacagcgat ccagaaaagc caaagccgtt cctttgcatc
      241 aataaacaga accgggttgc agtccggaaa acccggcgtg tccgccatga tgaatacggg
      301 gctccactgg gagtcgcctt ttctaagccg ggcaccctga acctgcacgt catccgctcc
      361 tctttctcct gagccgtgaa accaggcggc gagaagatcg ccgtccggag tttcgacaat
      421 gctgcttgca tggacgtgct tgtcctgaag cgggaaaatg tattcgcttt caaaagcggc
      481 tccgaagata ccggctgcta ctaccagtcc ggaaagaatc actgacatat ccaactcctt
      541 attcagaaca gcggcgtatc agaccgtgcc tgatgttctg tttattcatc tacatgaaag
      601 gtgatttgag aaaagacggg aaccggcgac agggcttcgc ggcctcctaa gggaagaagt
      661 tcaatgacaa aagcggcttc ctgcacagtg ccgcccgccc gttttaccag agagcacgca
      721 gcggaaacag tgccgcccgt tgccagcaga tcatccagaa tcagcacacg ccacccggga
      781 agaagggcac ctgtatggat ttcaatagca tcggtgccgt actccaggtt aaaactttcc
      841 cggatcgtat ccgcgggaag ttttccgggt ttacgcgcca tgattaaagg cacgccgagc
      901 cgcacggcaa gcgcaccacc gaagacaaag ccccgggatt caacagccag tatggcatcg
      961 ggtttatcgg cggcgtagcg gtttgcgaag acatcaataa cctgcaaaaa agcctccgga
     1021 tttaatacca gcgtagtgat atctttaaac tgaacaccgg gtttcgggaa attgggtacg
     1081 ttgcggatga cctcggataa atccataaaa gtatgctctc ctataggtaa tgagaagggg
     1141 cgcgtgtatt caccatgcta ccatgatccg ggcttttata ccaaaagaat caggcagaga
     1201 ctttcttttc tttgcctgtt cctgttattc tgtacaaact aaaaaaagcc ggtgccgcgg
     1261 cgtttccttc ctgaattact ctccaattat cagtcaatac agttatgaca caactacccg
     1321 aattttcaat aaccgcaacg ctgaaaaatg cctttgataa aaagccgaag ggcgctgttg
     1381 atctcttcgg ggttctcggt gccgcaaaag cgctggtacc tttgcaactc gcccgaaaga
     1441 tggaacacgc tcttttgctg attgcttcca gccgtaatga agccgaagca ctctatgaag
     1501 atctggccag cagtgctggg gaagaggcgt gtttgcactt tcccgcctgg gaagtatcgc
     1561 ccgatgaaat tatggatccc gccggagata ttattgccga acgtattcat accctcgagc
     1621 gtatggcgga aatgtcagaa aggaagaatc ctttttatgt tgtcgcgtcc gttcaggctt
     1681 ttttgcagta tgtaacgtcg ctgaagtcgc ttcaggacaa aatactgcat gtccgtacag
     1741 gcgaaatact tcccatggaa acgctcatag accacctcct gcaacgtggc tatacccggg
     1801 aagtcatggt ggaacagcgg ggcgatttca gtgtgcgcgg cggaattctc gatgttttcc
     1861 ccatgtccgc cgagttgccc tgccgtctgg aatattttga cgatgaaatc gagtcgatcc
     1921 gtgctttcga accggaaaca cagcgcagcg tcacccgtct tgagactgtc aaaattccgc
     1981 cctgctcgga aaaagatgcc ctgaaagagg cacatcactt gaagtcgctg gtgcctttca
     2041 gtgcctgttt tcccccagat actcttgtgg ccaccgtgga gtctctggca gtgcgggata
     2101 aagcggttgc tctcacggag caatggagcc aaagtccttt tttcatgacc tggcagcagg
     2161 cacaggccca tctcgccaaa ttccccaggc tgaccctgag ccagatgcct gaacaacggc
     2221 aggaggcgtc aagccgtttt gccatgcaaa tgagcatggt tgagaatttt tccggacgaa
     2281 ccaacgaatt ctgggagcag atccatgcct gggacatgca ggactatcgg gtgcacatcc
     2341 tctgtgtcaa ttccggcgag cagcgacgct ttatcgagct gcttcatgaa cacggctacc
     2401 ggccggaacg cgattcccgg attacggtct cccttggaag aatacatgcc ggttttgttt
     2461 ctcatgcaga caagatggtt ctcctcagtg agaaagaggt cttcggcaga cggtatgtgc
     2521 gcaggaaacg gaggcgcttt gaggcaggag cagccataac cgaatatgcg gatcttcgtg
     2581 ccggcgatta tattgttcat gaggtgcatg ggattggccg ttatcagggg ctgcgccgcc
     2641 tggaaggacg gagcgttgac tacctgacgc ttcgctatgc cggaggcgat accatttatg
     2701 tgcctgttac acagctggat cagatccaga aatacatggg cggagacggc gtgctgccga
     2761 aaatggacag gctcggcggt gccacctggt cggcgaccaa agcccgagtg cgccgtgctg
     2821 tccgggaaat gacagccgaa ctggtgagat tatatgccgc ccgtgagagc agcgaagggt
     2881 atgctttcac acgggatacc ctatggcaga cggaatttga agatgccttt gaatatgaag
     2941 agacgcctga tcagcgccgc gccattgatg aggtgaagcg ggatatggaa tccgaccggc
     3001 ccatggatcg gcttttgtgc ggtgacgttg gctacggtaa aacggaggtg gcgcttcgtg
     3061 ctgctttcaa ggcggttatg gacaaaaagc aggtcgccat gctggcgccg acgacggttc
     3121 ttgtgcagca gcattacaat acatttcggg aacgctttgc cgcctacccc atcacggtgg
     3181 aagtgctcaa ccgctttcag tcgggacggc agatcaggag cacgctggaa cgtctgaaaa
     3241 gcggggaagt ggatgtggtg gtggggacac accgcctttt atcgaaagat gtgggattca
     3301 gagatctggg gcttgttatt atcgatgagg aacagcgctt tggtgtccgg cacaaggaac
     3361 gactgaaaga aatgcgcacg cacgtcgatg tgctgaccat gtccgccacg cccatcccca
     3421 gaacccttca tttttcgctt ctgggcatcc gcgatatgag cgtgatcaat acggcaccca
     3481 atgaccgtct tccgattcac acctgtatag acgcctggga caaagaactg attcgggagg
     3541 cgattacccg ggaacttgca cgggacgggc aggtgttttt tctgcacaat cgcgtccaga
     3601 ctattgacag agttgccgcg tttttaaagc agatcgtgcc tcatgcccgt gtgggtatcg
     3661 gtcacgggca aatgtccaaa acggagcttg aatccgttat gaccggcttt attaaccatg
     3721 agacagacgt tcttctgtgc acgaccatta ttgcttccgg catcgatatt cccaatgcca
     3781 acaccatcat tgtggacagg gcggatcagt tcgggctcag ccagctatac cagattcgcg
     3841 gacgtgtagg cagatataaa caccgtgcct ttgcttatat gctcatcccc ggagaccggt
     3901 cgctcacttc ggaggcgcaa gcccggctgc aggcgcttca cgatttctcc gccttgggat
     3961 cagggtaccg cattgctatg cgggatatgg aaattcgcgg aacaggcgac ttgcttgggg
     4021 ccgaccagtc gggacacatt gccactgtgg gctatgaaac ataccgtgag atgatcacag
     4081 aagcagtggc ggaagcgcag gggaaaagtt tgtacaaaag atcgctgcct tcctttgacc
     4141 tggcggtgga tgccttcatc cctgatgctt atatgcctat tgcctctcag aaaattatcc
     4201 tgtacaggcg tattgcggca ctgaaaacgc ttgaagaagt gcaggagctg caggaagaac
     4261 tgcgggatcg cttcggtcct gttccgcgtc cgctgcgccg ccttctgcag atcatggaag
     4321 cacgggcgcg cgccgctgat ctgggcattg ccgcactcga agcaggacaa agagccgcaa
     4381 cagtttactt tgagacaggc agaacgctgg ctcagggcgt gcaggcacag ctgcgccgca
     4441 tgtatggcga tacgatcgtt ttctcctgga aagataaacc ggcgatgtcc ctgacctatg
     4501 acaatataga acagggactt gaaaacttca gtctgctcct cagacaactg catgccatga
     4561 tgactgaaga ttccatggaa gaagaggatc cgctactcta cgggctgtaa actatatcct
     4621 cttgactccc gggcagtgct tactatgtag ccgccattct ttcgatccgc ctgcaatcgt
     4681 atggcgccgt gccagtctgt ccgcagcagc gttatttttc gtgcttcaaa acgctctgcc
     4741 atggcgggtg tcatcagtgt tccccgtgag cctgcccggt gtgctgaaac aacggccaga
     4801 gaaggccgga cggcatcgag aaactcaatt cgacttgaag tgggggagcc gtgatgggcc
     4861 acctttaata catcgaaggg cgtctgaatg catggctgga tttctttttc gcctgcctct
     4921 tcaatatccg cggcaaacag gatacgtatt ccatcaaatg ccacggcaag cacgaggctt
     4981 ccgtcgttgc gtgacagatt gtccgtattg tgcaagggcg gatgcagcac ttcaacggat
     5041 gctttttccg ctggaagaaa atcgccccgg gaaagatgca gtacagggat attcttctgt
     5101 gcacacaagg cgatcaggtt gctttccaga gcgttgtaat catcccgaac cgctcccagc
     5161 accacttttt ttacagggaa atggttcagg acgtaaaatg cgccccctat atgatcccgg
     5221 tctgcatggc tgatgacaag atagtccaga tgtgaaatgc ggttggcgcg cagataggga
     5281 actatcatcc gcttgcctgc atcggtgtag ccgtctgaat cgccggcatc aatgagcaga
     5341 gaagtgccgc ccggtgtccg gacaaagctg ctgtcgcccc gtcccacatc cagaaaatcc
     5401 accagagcag gggcggtcca gggacgccag aaaaccagac aggcgcatac gcacaaaatg
     5461 cacagaaggc gattgcgccg atgctttcgt tcctcataaa ggaccaggta aaaaaagatg
     5521 agaaagagac agtaaaatcc aagagcgcac aatgtcggac ggtttacata aagaaatgag
     5581 acgggaagga ggagcgctgc tctgttgacc cactggataa gggacacaag aggaagcagc
     5641 gcatggccgg gcagcagtgc cagggttgat gatatggggg cgagtattcc tgttgcc
//
LOCUS       DAOPWB010000172         5662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_14234_length_5662_cov_4.587302, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000172 DAOPWB010000000
VERSION     DAOPWB010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14234_length_5662_cov_4.587302"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1153)
                     /gene="hisC"
                     /locus_tag="PKN92_11990"
     CDS             complement(101..1153)
                     /gene="hisC"
                     /locus_tag="PKN92_11990"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNZ49554.1"
                     /translation="MRFGRKIYESVEGYTPGEQPREPGIIKLNTNENPYPPSPRVIEA
                     LQALTADRARRYPDPLASALCRACAGVYNLPGEDWLVAGNGMDELLALALRAFVDPGD
                     TVLALYPTYTLYEVLCQIHDCRLQYIELNDEWLPPESFYKEKGRLCFLTRPNAPTGVA
                     MPRADVERFCAQFPGIVVIDEAYVDFCDDHCMDFPQRFDNVIVMRTFSKSYSLAGMRI
                     GIAAAQPPLIREFMKIKDSYNMNAWSQAAALAAVEDQAYMRSCAAKVRATRERLREAL
                     IGLGFRVPPAQTNFLLARWEGSPSASAIFEKLRREHRILVRYFNYRRLDTALRITIGT
                     DEECDALVDALSAILR"
     gene            complement(1156..2472)
                     /gene="hisD"
                     /locus_tag="PKN92_11995"
     CDS             complement(1156..2472)
                     /gene="hisD"
                     /locus_tag="PKN92_11995"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNZ49555.1"
                     /translation="MKFFDIQSQADLEQVQQFIVDSADRSSVEDSAEERRRNVQTLID
                     DVRRRGDTAVAEFTRRFDHVDLEPSQFELTEEDIDRAWDAVPRPVIAMLERAHENIRS
                     FHAKHLRGSWEEHLPDGSMYGQRLTPVENAGVYVPGGKAFYPSSVLMNIAPARVAGVS
                     EIVMVSPPSCEGSIHPAVVAAARIAGATRIFRVGGAQAIAALAYGTEHIPQVVKITGP
                     GNAYVTAAKALVRGIVDIDSEAGPSEVVVIADKTANPHHVAAELLAQAEHDEEALCLL
                     FTTSREVAEKTLARIEDRMQVLSRAPIIRESLSRFGAVVVTPDLETAVALTNLCAPEH
                     LSVQTEYAPRVADKVTHAGAIMLGAMTPVAVGDYYAGPNHILPTMRRARYSSPLSAED
                     FRKVTNVMFFSKERLNRDAHDIEEMARLEGLEAHAESVAVRIKDVN"
     gene            complement(2503..3756)
                     /gene="murA"
                     /locus_tag="PKN92_12000"
     CDS             complement(2503..3756)
                     /gene="murA"
                     /locus_tag="PKN92_12000"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNZ49556.1"
                     /translation="MDKMVIRGGNPLQGSVHVRGAKNAALPLMAASILAETPLTLHNV
                     PCLHDVFSMDKLLASMGVYSEFTGRYMILDSRGIVTPEAPYDLVRKMRASFFVLGPLV
                     ARFGRARVSLPGGCAIGTRPVDIHLKGLEALGCRIELDEGYVVAEGKLTGASFALDFP
                     SVGATETLMMAATRAEGLTRLYNVAREPEIEDLAACLNAMGAQISGAGSDMITIVGVD
                     SLKGAEHEVIPDRIEAGTFLIAGVATHGDVTVLNARADHLPGFIEKLREIGAQVDVQQ
                     DRIRASAANGYRAADITTRPYPGFPTDLQAQTMALLTCAEGASMIKETVFENRFMQVA
                     ELNRMGAEIDVDGSTALVRGVPTLMGAPVMASDLRASAALVIAGLMAPKGETAVQRVY
                     HIDRGYERIEERLAALGADIERRRN"
     gene            complement(3918..4454)
                     /gene="secG"
                     /locus_tag="PKN92_12005"
     CDS             complement(3918..4454)
                     /gene="secG"
                     /locus_tag="PKN92_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNZ49557.1"
                     /translation="MLSIIFSLNTLWWLILLLYIPACFALIIIVLLQKGKGQGLAGAF
                     GMGGGMDTIFGPRSSKSLPQKITYAGAAVFMVLALVMSILSGKVGKAAAPELAEAPVQ
                     ASLDALFDGSAAEAPADASEAAGGITEVTVNPDTPAEGAVAAPTETAEEAAAAEAPAE
                     AAVEEAGDKEEAGGGQAE"
     gene            complement(4526..5287)
                     /gene="tpiA"
                     /locus_tag="PKN92_12010"
     CDS             complement(4526..5287)
                     /gene="tpiA"
                     /locus_tag="PKN92_12010"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNZ49558.1"
                     /translation="MSTRKPFVAGNWKMNNFVADAVSLAEGLKALVSGVDTVDIAVCP
                     TYTSLYAVGKVLAGSTIQLGAQDAYVKASGAYTGEISPQMLQDVGCTWTIIGHSERRH
                     ILGESDEFLNEKLRFALASGLKVMFCIGELLEERKGGTMEAVLERQTTKGLDGLSADV
                     AAGVVIAYEPVWAIGTGETATPDQAEDAHIFVRGVVEKMFGKDCADRVRIQYGGSVKA
                     DNAAELIAREHIDGFLVGGAALKADSFAAIINACK"
BASE COUNT         1320 a         1720 c         1435 g         1187 t
ORIGIN      
        1 atgcacctgc tataatgaac gtaatgcacc gtctatgata acacatactt tttcagaaga
       61 caatccgaaa aaactttgta tccgaaaaaa ctttgatgat ttaacggagg attgcggaca
      121 atgcatccac cagggcgtcg cattcctcat cggttccgat cgttatccga agggcggtat
      181 ccaggcgccg atagttaaaa tagcgcacaa gaatgcggtg ttcccggcgc aatttttcaa
      241 aaatggccga cgccgacgga cttccctccc aacgcgccag cagaaaattg gtctgcgccg
      301 gaggcacccg gaaacccagc ccgatcagcg cctcgcgaag ccgttcccgt gtcgccctta
      361 ccttggccgc acagctgcgc atatacgcct gatcttcaac ggccgccaac gctgccgcct
      421 gactccaggc attcatattg taggagtctt tgatcttcat gaactcacgg atcaggggcg
      481 gctgggcagc agcgataccg atgcgcatcc cggcaagact gtaggacttt gaaaaagtgc
      541 gcatgacaat gacgttgtca aaacgctgcg gaaaatccat gcagtgatca tcgcagaagt
      601 ccacataggc ctcgtcaatc accacaatcc ccggaaactg ggcacagaag cgttccacat
      661 ccgcccgggg catcgccacg cccgtcggtg cattgggccg ggtaagaaaa cagaggcgcc
      721 ccttttcctt gtaaaaagat tcaggcggaa gccattcatc gttaagttcg atatactgca
      781 gcctgcagtc gtggatctgg caaagcactt catacagcgt ataggtagga taaagggcaa
      841 gcaccgtatc cccggggtcg acaaaagcac gcagcgccag cgccagcagt tcgtccatac
      901 catttcccgc aacaagccag tcctcacccg gcagattgta aacacctgca caggcacgac
      961 acagcgccga agcaagcgga tccggataac ggcgcgctct atctgcagta agtgcctgaa
     1021 gcgcttcgat cacccgtggc gacggcggat aggggttttc attggtattg agtttgataa
     1081 taccgggttc ccggggctgt tcgccgggcg tatagccttc cacactttca taaatttttc
     1141 gaccgaaacg catggtcaat taacatcctt tatccgcact gccaccgatt cggcatgggc
     1201 ttccagtcct tccagtcgtg ccatttcctc aatatcatga gcatcacggt tgagccgttc
     1261 cttggagaaa aacatgacat tggtcacttt gcggaaatct tccgcagaca gcggcgagct
     1321 gtaccgcgcc cgacgcatgg taggcagaat atgattgggc cccgcatagt aatcaccgac
     1381 agccacaggc gtcatggcgc ccagcatgat cgcacccgca tgggtcacct tatccgcaac
     1441 ccggggcgca tattcagtct gcacactcag gtgttccggc gcacagagat tggtcagggc
     1501 aactgccgtt tccagatcag gcgtaaccac cacggctccg aaacgggaca gcgattccct
     1561 gattatgggg gcgcgggaca gcacctgcat ccgatcttct atacgggcga gggtcttttc
     1621 cgcgacttcc cgggacgtgg tgaataataa gcagagcgcc tcttcatcat gctcagcctg
     1681 tgccagcagt tccgccgcca catggtgcgg attggcggtt ttgtcggcaa taacgaccac
     1741 ctcggaggga cctgcttcgc tgtcaatgtc cacaatgccc cgcaccagtg ctttggcggc
     1801 agtgacgtag gcatttccgg gaccggtaat tttgaccacc tgcggaatat gctctgtacc
     1861 gtaggccaga gcggcaattg cctgagctcc gccgacacgg aagatacggg ttgcgccggc
     1921 aatacgggct gcagcaacga ctgcgggatg gatggagcct tcacagctgg gcggtgacac
     1981 cattacaatc tcgctgaccc cggcaacacg ggcgggagca atattcatca gtacggatga
     2041 aggataaaag gccttgccac cgggcacata aacacccgca ttttccaccg gggtcaggcg
     2101 ctgaccgtac atggagccat cgggcaaatg ttcttcccag gatcctctca gatgcttggc
     2161 atgaaagctg cggatgtttt catgggcgcg ctcaagcatg gcgatgaccg gtcggggcac
     2221 agcgtcccac gccctgtcaa tgtcctcttc cgtcaattca aactgagacg gctccagatc
     2281 aacatgatca aagcgccgtg taaattctgc cacggccgta tcaccccggc gacgcacgtc
     2341 gtcaatcagt gtctgcacat ttctgcgccg ctcttccgcc gaatcttcca cggaagaccg
     2401 atccgcactg tccactataa actgttgtac ctgctccaga tctgcctgag actggatatc
     2461 aaaaaatttc ataatcggtt cttgctccat accgtatctc tgtcagttcc gccgacgttc
     2521 aatatcggcg cccagtgctg caagccgctc ttctatgcgc tcgtatccgc gatcaatatg
     2581 ataaacccgc tgcactgccg tttcaccttt cggcgccata agccccgcta tcaccagcgc
     2641 tgcactggca cgcagatcgc ttgccatcac cggcgcaccc ataagagtgg ggaccccccg
     2701 caccagcgcc gtgctcccgt ccacatcaat ctcggcaccc atgcggttca gttctgcaac
     2761 ctgcatgaac cggttttcaa aaacagtttc tttgatcata ctggcgccct cggcacaggt
     2821 cagcaatgcc atggtctgcg cctgaagatc ggtgggaaag ccgggatagg gccgggtggt
     2881 tatatccgcc gcacgataac cattagccgc tgacgcacgg atgcgatcct gctgcacatc
     2941 cacttgcgca ccgatctccc ggagtttttc tatgaatccg ggcaggtgat ccgcccgggc
     3001 attcagcacc gttacatcac cgtgcgtcgc cacaccggca atcagaaagg tgcccgcctc
     3061 tatccggtcg gggatgactt cgtgctcggc acctttgaga ctgtccacgc ccacaatcgt
     3121 gatcatatcc gagcctgcac cggaaatctg ggctcccatg gcgttcaggc aagcggcaag
     3181 gtcttcaatt tcaggttccc gcgcaacgtt atacaggcgg gtcaatcctt cggcgcgggt
     3241 cgccgccatc atgagcgttt cagtggcgcc gacactggga aaatccagtg cgaaggaagc
     3301 gcccgtcagc ttgccctcgg caacaacata gccctcgtca agctctatcc ggcagcccag
     3361 tgcctcaagc cccttcaggt ggatatcgac aggccgtgtt ccgatggcac atccgccggg
     3421 caacgaaacc cgcgctctgc cgaaacgcgc caccagcggg cccagcacaa aaaaggaggc
     3481 acgcattttc cgaaccagat cgtacggcgc ttcaggagtg acaatccccc tgctgtccag
     3541 aatcatatag cgtccggtaa attcactgta cacgcccatc gatgccagca acttgtccat
     3601 ggaaaaaacg tcatgaagac aaggaacgtt gtgcagcgtg agcggtgtct ccgccagaat
     3661 cgacgccgcc ataaggggaa gggctgcatt tttggcgcca cgcacatgca ccgatccctg
     3721 taaagggttg ccgcccctga ttaccatttt atccatcgct gtgtgctcca gtcagcagct
     3781 gccctgcctg cttccgtctg tcagcagaaa atacctgact gtaatccgga atgccgtgct
     3841 ataaaagaca gcccaaagcg taaaatgcct tgggctgtac agatcagtat tgaatgctta
     3901 agcgcggggg gaacagatta ttcagcctgc ccgccgcccg cttcttcttt atcgccagcc
     3961 tcttcaactg ctgcctccgc aggcgcttct gctgctgccg cttcctcagc agtctccgtc
     4021 ggagctgcca cagcgccttc agcaggcgta tccggattca ccgtcacttc tgtgataccg
     4081 ccagcggcct ccgaggcatc cgcaggggcc tctgctgcgc tgccatcaaa cagcgcatca
     4141 agagacgcct gaaccggtgc ttcagcaagt tcgggggctg ccgctttccc gacctttccg
     4201 gacaggatgg acatgacaag ggcaagaacc ataaacacgg ccgcaccggc ataggttatt
     4261 ttctgaggca ggcttttgga ggaacgggga ccgaaaatcg tatccatgcc gccgcccata
     4321 ccaaaggcac ccgccagacc ctgccccttc cctttttgaa gaagcacgat aatgatcaga
     4381 gcaaaacacg ccggaatata aaggaggagg atcaaccacc agagtgtatt caggctgaag
     4441 ataatactta acatgctgtt cattcccttg cgaagttggc tcatgggccg cccggtaccg
     4501 gaatagtgcc ggggttctga ttctgttatt tgcaggcgtt gataatggcg gcaaaactgt
     4561 ctgctttcaa ggccgctccg cccacgagga aaccgtcaat atgttcacgg gcaatgagtt
     4621 ccgccgcatt gtcggccttc acgctgccac cgtattgaat acgcacccgg tcggcacagt
     4681 ctttgccgaa cattttttca acaacaccgc gcacaaaaat gtgggcgtct tccgcctggt
     4741 ccggcgtggc ggtttcgccc gtgccgatcg cccagacagg ttcataagca atcaccacgc
     4801 ccgccgccac atcagcgctc aggccgtcca gtcccttcgt ggtctggcgt tcaagcaccg
     4861 cttccatggt gccgccttta cgctcttcca gcagctcgcc gatgcagaac atgactttca
     4921 gaccgctcgc cagcgcaaag cgcagctttt cattgagaaa ttcatcgctt tcgcccagaa
     4981 tatggcgacg ttcgctgtga ccgataattg tccacgtgca gcccacatcc tgcagcatct
     5041 gtggcgaaat ttcgccggta taagcgccgc ttgccttcac ataggcatcc tgtgctccaa
     5101 gctgaattgt ggatccggca agcaccttgc cgacagcata cagagacgta taggtcggac
     5161 aaacagcaat atctaccgtg tccacgcccg ataccagcgc tttaagacct tctgccagac
     5221 tgacagcgtc ggcgacaaaa ttattcattt tccaattgcc ggctacaaat ggtttgcgtg
     5281 tggacatgtt tgttctatct ttcttgtaaa tcagggctgt aaaaagaaaa gacccggata
     5341 gggcacatca agcaacaggc acaactcatc cttgggaagg ctctgcctga gaagaaaaag
     5401 atagcacaga accggggctt aaagcaagca gagcggccac ttcaacagga aacagataca
     5461 cttttctctt taatacatgc cagagagcct tttctcttaa ataaagtcgc tgcatgtttt
     5521 agagcgccct gcggaacgtt cagaaacgcc aagcgaccca ttgcgacaca aacgggtcat
     5581 gaagaagaca gtcactcgga tctgtatcgg aaatacgtcc tgcgacatac acacaacatc
     5641 acagaccaca cacgcccctg cc
//
LOCUS       DAOPWB010000173         5584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_14489_length_5584_cov_5.744438, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000173 DAOPWB010000000
VERSION     DAOPWB010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14489_length_5584_cov_5.744438"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKN92_12015"
     CDS             <1..253
                     /locus_tag="PKN92_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49559.1"
                     /translation="AVFFLTRLDANAEIGVPRRSRFLAFGLNRLLRAFVVNSETVLIS
                     NPALRCFPGFLMHSLDQPLISHIQCVYGLIIPFFIFFPD"
     gene            262..993
                     /locus_tag="PKN92_12020"
     CDS             262..993
                     /locus_tag="PKN92_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ49560.1"
                     /translation="MSTASDHTGNADTVIRVEGLYKRFVMGDTVVRALDGVSFTISRG
                     SFVAIMGPSGSGKTTLLDILGCLSRPTEGDYWLAGSKVSNLSDRQLARVRNHEIGFVF
                     QNFNLLARSSALVNVELPLFYDGVRRRERTQRAIAALTAVGLENRLHHCPNELSGGQR
                     QRVAIARALINSPSIIFADEPTGNLDTASGSSIMGLFENLHQKGHTIVMVTHERDIAE
                     HAERIMSFRDGRLVTDEWLHPKHSN"
     gene            966..2321
                     /locus_tag="PKN92_12025"
     CDS             966..2321
                     /locus_tag="PKN92_12025"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ49561.1"
                     /translation="MAASKTFKLIRTLIGWIIFLSLIGGPAAYFYLKEPIIDVTVITL
                     KRGPIEQTISAFSSGTVEPKVSSMVAPESIGKVMAVHVKKGQRVEKGDVLVELDCTQQ
                     SLQVAQAEARIAQANIGIRLLKEQYGNDQGRIELLKRTRDLAAHEFGKDKKLLEKDDV
                     GSESMVNLSEINYNQIDDTYKNLSNLVSLYPLRIQEAETALNGATLLLEQAKVAAEWT
                     KVCAPFSGLIADTMVEVGEVVGGGLGAGLSGGLGGGAMMGGGMGAMGGAALAAGGGGG
                     GLGGVSPLAVVHLVDDSDMYVKAPFDESTFGKLELGQPVRLTFDPYPDEEFPGRVSHI
                     AATVTKNLDRSRTFEVEILIEKGKERLIPGMSADAVIIANSKDNVLYVPTEALIREEE
                     GYVVEDGRAVRRKVTMGIGNWMTREVLDGLRENEVLITSVSVKGLRDGAKVNILSTLE
                     Q"
     gene            2321..3535
                     /locus_tag="PKN92_12030"
     CDS             2321..3535
                     /locus_tag="PKN92_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ49562.1"
                     /translation="MNILECIWMALTSLSENKVRSFLTTLGVIIGVMSVILLVSLGEA
                     AQAYIEKQFSGMGSNVILVTPGKQETTSSMIAVSAGSFRKLTTDNAAEIRRKAQGVKA
                     VAANVIGTGQVRFRSVERNCIVLGTEEGLAEIRDVHTTQGRFISAQDIEKNNKVCVIG
                     LKLKNDLFGSSPALHQRISINRSKHVVVGVLEERGQMLGIDLDDIVVIPLPSGQQMFY
                     GGEDTIFEINASAKTPEDIPLAQESIRKVLKAAHDNNEDFTIINQDAIVDSFGKILSA
                     LRLMLVGIAAISLLVGGIGIMNIMLVSVRERTREVGIRKSVGATRSDIALQFLIEAVT
                     LSVCGGLIGVGIAWAATVILGIVYPSVPVTISLWSVLTAFLFSLSTGVFFGVYPATKA
                     SSVDPVEALRYE"
     gene            3635..>5584
                     /locus_tag="PKN92_12035"
     CDS             3635..>5584
                     /locus_tag="PKN92_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49563.1"
                     /translation="MNIRTFCCLLIVTCLAVCSLHGETIPLTEGWYLRSSDGLAVSGA
                     EVSRDGFSTQDWYALDRPRTVLSALAKVGVYADPYYGENLKSIPGYQDGMWLRHLDDS
                     PFRVPWWYRIEFTPAPLQQDTCFTLHLDGINYKANIWLNGKKIAGADQVTGMFRRFEF
                     PVTDHLRFNEKNVLAIEIIPPGLLPEKHVNTKQLEATTGWDDHNPQPPDMNMGIWQPV
                     YLKTQGPVSLRHPYVDSDLSLPGMESARLTALAWLKNNTNQPQTCLFSGIVEGRQVSQ
                     EVTLAPGEEKEAVLAPEQHPELILQSPRVWWPHPAGEQPLYDARFSVSVNGRESDSAT
                     LRFGIRSAESYINAEDWRGYKINGRNILIRGGAWMTSDMLLNLEDSRYEALVRYAREA
                     NLNMLRSEGFSIRETESFYDLCDRYGIMVTQQLFGRNLPDEALAIACVEDTLLRIRNH
                     ASLVHFLGHDETFPTDTLDAAYQHLLEKHRIKRSYQPHSGTFNIATRAKTGGTRTGTR
                     ELWTYAGPSHYFFTRERRFDSAWGFAQSGGIGGILAAQDSLRQMMPASALWPIQQNEC
                     WSFHTVTQGMEYFNAVIQAMEQSYGAAADFNDFCRKLYAMNYNSARGMFEAYARFKYD
                     ALGITTWKYNAAWPAALTWQYVDWYT"
BASE COUNT         1276 a         1468 c         1555 g         1285 t
ORIGIN      
        1 ggctgtcttt ttcctgaccc gcttggatgc caatgcggag attggcgttc ccaggaggag
       61 tcgctttctg gcttttggat taaacaggct attacgcgct tttgtggtca atagtgaaac
      121 cgtacttatt tccaatcccg ctctgcggtg ctttcccgga tttttgatgc actctcttga
      181 tcagcctctc atcagtcata tacaatgcgt atacggttta attattccct ttttcatatt
      241 ttttccggat tgattcctta catgagtact gccagcgatc ataccggcaa cgcggataca
      301 gtcatccgcg ttgaagggct ttacaaacgc tttgtcatgg gcgataccgt cgtgcgggcg
      361 ctggacggcg tttcttttac catttcccgt ggcagttttg ttgccatcat gggtccgtcc
      421 ggttccggaa agactacgct tcttgatatc ctcggatgcc tttcacggcc tactgaaggg
      481 gattactggc ttgccggatc gaaagtgagc aatttatccg accgccagct tgcccgcgtg
      541 cgtaaccacg agatcggctt tgtatttcag aattttaacc tgcttgcccg ttcaagcgcg
      601 ctcgtcaatg tggagctacc tctcttttac gacggagttc gccgcagaga gcgcacccag
      661 cgtgccatag cggcgctgac agctgtaggc ctggaaaatc ggctgcatca ttgtcccaac
      721 gagctttccg gcgggcagcg ccagcgtgtt gccatagccc gggctttaat taattcgcct
      781 tcaatcattt ttgccgatga gccgacaggt aacctggata cggcaagcgg ttcaagtatc
      841 atgggccttt tcgaaaatct tcatcaaaaa ggacacacaa ttgtcatggt cactcatgaa
      901 cgggacattg cggaacatgc agaacgtatc atgagttttc gtgacgggag gttggtaacc
      961 gacgaatggc tgcatccaaa acattcaaac tgatacgcac tctgatcggg tggattatct
     1021 tcctttctct gattggcggg cctgccgctt atttttacct gaaagaaccg attattgacg
     1081 taacggtcat cacactgaaa cgcggcccta tcgagcagac gatttccgcg ttttcatcag
     1141 gcacagtaga gcccaaagtt tcgtccatgg tggcgccgga gagcataggc aaggtcatgg
     1201 cggtgcatgt caaaaaaggt cagcgggtcg aaaaagggga tgtgctggtg gaactggact
     1261 gcacgcagca aagccttcag gttgcccagg cagaagcacg gattgcacag gcaaacatcg
     1321 gcatacggct gcttaaagag caatacggca atgaccaggg tcgcatagag ctgctgaaac
     1381 ggacccgcga tctggcggca cacgaattcg gcaaggacaa gaaattgctt gaaaaggacg
     1441 atgtcggctc tgagagcatg gtcaatctct ccgaaatcaa ctacaaccag atcgacgata
     1501 cctataagaa tctcagcaat cttgtatccc tctacccctt acgcattcag gaagcggaaa
     1561 cagcactgaa cggtgctacg ctgttgctgg aacaggcaaa agttgcggct gaatggacga
     1621 aggtgtgtgc gcccttctcc gggctgatag cggacaccat ggtcgaagtg ggtgaagttg
     1681 tcggcggcgg tcttggtgca ggtctgagcg gcgggctggg cggcggtgcc atgatggggg
     1741 gcggcatggg cgccatgggc ggtgcagctc tggcagccgg cggaggcggg ggcggactgg
     1801 gcggtgtatc ccccctggct gttgtgcatc tggtggatga ctccgatatg tatgtcaagg
     1861 cgccttttga cgagtccacc ttcgggaagc tcgaactggg tcaacccgtc cgcctgactt
     1921 ttgatcctta tcccgacgag gaatttccgg gccgggtgtc gcatatcgct gccacagtca
     1981 ccaaaaatct tgatcgaagc cgcacctttg aagtagagat actgatcgaa aaaggcaagg
     2041 aacggcttat acccggcatg tccgccgatg ctgtcatcat tgcgaattcc aaggacaatg
     2101 tgctttatgt gccgacagaa gcgctgatcc gtgaggaaga aggctatgta gtggaagacg
     2161 ggcgtgccgt gcgccgtaaa gttacgatgg gcatcggcaa ctggatgaca cgggaagtgc
     2221 tggacggtct tcgtgaaaat gaagtgctta tcacctccgt cagcgttaag ggtctgcgtg
     2281 acggtgccaa agtcaatatt ttaagtacgc tggagcagta atgaacatcc ttgaatgtat
     2341 ttggatggca ttaacctctc tgtcggaaaa caaagttcgc tcttttctga caacgctggg
     2401 cgtcattatc ggagtgatgt ccgtgatcct gcttgtctct ctgggtgaag ctgctcaggc
     2461 atacattgaa aagcagttca gcggcatggg cagcaatgtt attctggtca cgccgggcaa
     2521 gcaggaaacc acctccagca tgatcgcagt cagtgcgggc agcttccgca aactcaccac
     2581 ggataatgcc gccgaaatcc gaagaaaagc gcagggcgtg aaagcggtgg ccgccaatgt
     2641 tatcggcacg ggacaagtgc ggttcagaag cgtggaacgg aactgtattg ttctgggcac
     2701 agaggaaggg ctggcagaaa tccgggatgt tcatacaaca caggggcgtt ttatctccgc
     2761 ccaggatata gagaagaaca acaaggtctg cgtgatcggg ctaaaactga aaaacgatct
     2821 gttcggctca agcccggcac tgcaccagcg catttccatc aaccgatcca aacacgtcgt
     2881 ggtaggcgta ctggaggaac gggggcagat gctgggtatt gatctggacg acattgtcgt
     2941 gatccccctt cccagcggtc agcagatgtt ctacggcggc gaggacacta tttttgaaat
     3001 caatgcttca gcaaaaacgc ctgaagacat ccctctcgca caggaatcga tccgcaaagt
     3061 actgaaagcg gcacatgaca ataacgagga tttcaccatt atcaatcagg atgccattgt
     3121 ggattctttc gggaagattc tcagtgcact gcgtctgatg cttgtaggca ttgccgccat
     3181 ttccctgctt gtcggcggaa tcggcatcat gaatatcatg ctggtatcgg tgcgtgaacg
     3241 cacccgcgaa gtgggtatcc gcaagtctgt tggagcgacc cgttccgaca ttgcgctgca
     3301 gtttctcatt gaagcagtaa cactgagcgt atgcggcggg ctgatcggtg taggcattgc
     3361 ctgggcagcg actgtgattc ttggaattgt gtatccctcc gtgccggtca ccatttccct
     3421 ctggtctgtt ttgaccgcct tcctgttcag tttatccacc ggtgttttct tcggtgtcta
     3481 ccctgccacc aaggcaagca gtgtggatcc tgtggaagcg ctgcgctacg aataaaaagc
     3541 gactgcgctc cttcccttct gaagaagatg cccggaaacc cgatgtacct tttcgtcgtc
     3601 atataaagac aggaaagccc gaaaggaaac atgcatgaat atccgcacct tctgttgtct
     3661 gctgattgtc acctgccttg ccgtctgctc tcttcacggt gaaaccattc cattaacaga
     3721 gggatggtat cttcggagca gtgacgggct tgccgtcagc ggtgcggagg tgtcccggga
     3781 cggattcagc acacaggact ggtatgcact ggacaggccc cgcaccgtac tgtctgccct
     3841 tgcgaaagtg ggtgtatatg ccgatcccta ttacggggaa aacctgaaaa gcattcccgg
     3901 ctatcaggac ggcatgtggc tcaggcatct ggacgacagt cctttccggg ttccctggtg
     3961 gtatcgcatc gagtttaccc cggcacctct gcagcaggat acctgcttca cactgcatct
     4021 ggacggcatc aactacaaag ccaatatctg gctgaacgga aaaaagattg ccggagcgga
     4081 tcaggtaacg ggcatgttcc gccgcttcga gttccccgta accgaccacc tgcgtttcaa
     4141 tgaaaaaaat gtgcttgcca tcgaaattat cccgcccgga ctgcttccgg aaaaacatgt
     4201 aaacacaaaa cagctggaag ccactacggg ctgggacgac cacaatccgc agccacccga
     4261 tatgaatatg ggaatctggc agcccgtata tctgaagaca cagggaccgg tcagcctgcg
     4321 ccatccctat gtcgacagtg acctgtctct tcccggtatg gaaagcgccc ggctcacagc
     4381 acttgcctgg ctgaagaaca acacgaacca gccgcagacc tgcctgtttt ccggcatcgt
     4441 ggagggacgg caggtatcgc aggaagtgac gctggcgccg ggagaggaaa aagaggccgt
     4501 gctggcccct gagcagcatc ctgagctgat cctccagtct ccccgggtct ggtggcctca
     4561 tccggcaggt gagcagcccc tttacgatgc ccgcttttct gtcagcgtta acggccggga
     4621 atcggacagt gccactctgc gtttcggcat ccgctcagcg gaaagttaca tcaatgcgga
     4681 agactggcgt ggctacaaaa tcaacgggcg caacatcctc atccgcggcg gtgcctggat
     4741 gacatcggac atgctgctga acctggaaga ctcccgttac gaagcgctgg tgcgttatgc
     4801 ccgggaagcc aatctgaaca tgctgcggtc tgaaggcttt tccatccgtg aaacggaaag
     4861 tttttatgat ctctgcgacc gctacggaat tatggtcacc cagcagcttt tcggccgtaa
     4921 tctgcccgat gaggcgctgg cgatcgcctg tgtggaagat acgctgctgc gcatccgcaa
     4981 ccatgcgagt ctggtacatt ttctggggca tgatgaaacc tttccgaccg atacgctgga
     5041 tgcggcttat cagcatctgc tggaaaagca tcgtattaag cgaagctatc agccgcactc
     5101 aggcaccttc aatattgcga cccgcgccaa gacaggcggt acccgcacag ggacgcggga
     5161 gctgtggacc tacgccgggc cgtcccacta ttttttcacc cgggaacgcc gttttgattc
     5221 cgcctggggc tttgctcaat ccggcgggat cggcggcatt ctggctgcac aggacagctt
     5281 gcgccagatg atgcccgcat cggcgctatg gccgattcag cagaatgaat gctggtcgtt
     5341 tcacacggta acacagggta tggaatattt taatgccgtg attcaggcaa tggaacagag
     5401 ttatggcgct gcagcggact tcaatgattt ctgcagaaag ctttacgcca tgaactacaa
     5461 cagtgcccgg ggcatgttcg aggcgtacgc ccgtttcaaa tatgatgcgc tgggcattac
     5521 cacgtggaaa tacaatgccg cctggcctgc agcgctcacc tggcagtatg tggactggta
     5581 cacc
//
LOCUS       DAOPWB010000174         5578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_14506_length_5578_cov_5.116603, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000174 DAOPWB010000000
VERSION     DAOPWB010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14506_length_5578_cov_5.116603"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKN92_12040"
     CDS             <1..817
                     /locus_tag="PKN92_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49564.1"
                     /translation="NWKRTGISFDEKQASGPSSGLRQRYLMTRFGQALSVLKTHKALS
                     LTYCFCAVLLLALFRTGLIAVGQQGGKGAASSLDALAGVGVMVLFVLAYSMMQAWFYG
                     RLGAGAGGTLWRWQGTVETLKRYVMPWLLLNLLSLTFADLTHRAALAGNTQTAGFFES
                     LTLCWHLISMPAGVCILFYRGLVWRELHEALVPLFRFPGALILPMALAFLQYLHSGVQ
                     AAFVDETAAGMLLLCAVTDIPQVILDLIIFVLYWRICMDDCLTPREEDDDYEF"
     gene            complement(840..2015)
                     /locus_tag="PKN92_12045"
     CDS             complement(840..2015)
                     /locus_tag="PKN92_12045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ49565.1"
                     /translation="MEKLKYILLFAALAAVGSMLVFRHHFSAPHETLPEHRPEKAAAH
                     SVHKNAAAAGHSVDEPEAEVVSGDVQQPAVDPAPTSQSPANNSVKQELPGKGGAAAPQ
                     KDNSKNKKPTDRTEKNKREDKGAAGQEKQAEALVDQAQTLIRRGSYQQAESLLRQSLA
                     LSGKNSNAWRELAHLYKKTGQIDQEMETFAEWSRAMPNNTESLIGLADVSMRQGNYEA
                     ARGYLAESEKRPDRIDQYGQIARLYRQMDDTGEEGRVLSGWMGAAPDSEEARKDWAGY
                     QRRQGDLEGALAEYGRLSEDFPEDASYQRRMGDIYRNMGDYPSALSHYQAALEKQPQN
                     PAFLIRLADTQTQMQDYQGAVETWSVIAALQPGSQQAALAERRIAELTGTPLPQPAP"
     gene            complement(2145..3974)
                     /gene="glmS"
                     /locus_tag="PKN92_12050"
     CDS             complement(2145..3974)
                     /gene="glmS"
                     /locus_tag="PKN92_12050"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNZ49566.1"
                     /translation="MSGVVGYIGGKNPVEVLVAGLYRIKGHDSAGVAVVNDEGVTAVK
                     SLGKLENLEAKLAAQPVTGHLGIGHARWATHGVPNETNAHPHFDALNEIAVVHNGIIE
                     NYQELRTSLQQEGVAFRSQTDSETLAHLIRKYYQGNLFKAVQQALRCAKGAWALGVVS
                     RHNPDVLIVARQGSPVVVGVGDNEAFMGSDVSAIIKYTRDVVYLDHGQICEVRRDGIK
                     VEDLDGNPVQHEVVHVDWDDSAAEKDGFPHFMLKEIHQQPEVIRNTLRGRISDDASDV
                     NLSDINISEADIKACPRITIVACGTSWHAGMVGKYLIERFAEIPVELDLASEYRYRDP
                     IVEKNAIVIPVSQSGETADTMEAVRLAKSQGARILAVVNTVGSSVARESNGVIYLQAG
                     PEVSIASTKAYTTQVTAFALLTIWLARIRGTMTKDQAKDMIAELRTLPDKMQLVLNNQ
                     EPIKALAGKQKYRDAQSAFFLGRDYNYPSALEGSLKLKEVSYIHAEAYPAGELKHGPI
                     ALVTNEMPVICVAPKGNIYDKMVSNIQSIKARAGLVISIATEGDTAIQAYSDDVLYIP
                     ECYEPFSPILVALPLQLFAYYVAVNRGCDVDQPRNLAKSVIVE"
     gene            complement(4106..5146)
                     /locus_tag="PKN92_12055"
     CDS             complement(4106..5146)
                     /locus_tag="PKN92_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HNZ49567.1"
                     /translation="MPLVPMRQILDEAAKGGYGVGAFNVNNMEQIQAIMQAANDTHSP
                     VIIQASRGALKYSKMIYLKKLMEAAAEEYPHIPVAMHLDHGNSLATCKEAIALGFTSV
                     MMDGSLSEDGKTPNSYEQNVKVTCDVVEYAHPLGVTVEGELGCLGGIEDGHGAGLSEA
                     EVQDHLTDPAQAEDFVAKTQLDALAVAIGTSHGAYKSGRKDPVTGEMLPPTLAMERIH
                     EIHDRMPNCHMVMHGSSSVPKELVDIINQYGGNMPDAFGIPVEQIQDGIRHGVRKINV
                     DTDSRLAITGAIRKVFVESPSKFDPRDYLKPAREAAYEVYVARMKAFGQAGHATDYTP
                     LTLQDMIEIYKK"
BASE COUNT         1071 a         1575 c         1565 g         1367 t
ORIGIN      
        1 gaattggaaa aggacgggta tatcattcga tgaaaaacag gcatcgggcc cttcatcggg
       61 tctgcggcag aggtatctca tgacacgatt cggacaggcg ctgagcgtgc tgaagacgca
      121 taaagcattg tcactgacat actgcttttg cgccgtactg ctgctcgccc tttttcgcac
      181 cgggcttatt gcggtcgggc agcagggcgg gaaaggagcc gcatccagtc tggacgctct
      241 tgcgggcgtg ggcgtgatgg tgcttttcgt gcttgcctac agcatgatgc aggcgtggtt
      301 ttacggacgg ctcggtgcag gggcaggcgg aacgctgtgg cgctggcagg gcactgtgga
      361 aacgctgaag cgttatgtca tgccctggct gctgctgaat ctgctttccc tgacctttgc
      421 cgatctgacg caccgtgcgg cgctggcggg taatacacag accgccggat ttttcgaaag
      481 tcttacgctg tgctggcatc tcatttccat gcctgccggg gtctgcattc ttttttacag
      541 ggggcttgtc tggcgcgagc tgcatgaagc gctggtgcct cttttccgtt ttcccggtgc
      601 actgattctg cccatggcac ttgcctttct tcagtatctg cacagcggcg tacaggctgc
      661 atttgttgac gagactgctg ccgggatgct gctgctctgt gccgtaacgg atattcccca
      721 ggtgatactg gatctgatta tttttgtgct gtattggcgc atctgtatgg atgactgtct
      781 gacgccccgg gaagaagacg acgactatga attttaacgg aggcacccgg cgccccgatt
      841 cagggtgcgg gctgcggcag tggcgtgcct gttagttcgg caatgcgtcg ttcagccaaa
      901 gctgcctgct gcgagccggg ctgcagtgct gcaatgacgg accaggtttc caccgccccc
      961 tgataatcct gcatctgtgt ctgtgtgtcg gcgaggcgga tgaggaaggc agggttttgc
     1021 ggctgctttt ccagcgccgc ctggtagtgg gataaggcgg acggataatc ccccatgttc
     1081 cggtagatat cgcccattct ccgctgatag ctggcatctt ccggaaagtc ttcggacagc
     1141 ctgccgtact cggcaagcgc gccttccaga tcgccctggc gacgctggta ccctgcccag
     1201 tctttgcgtg cctcttcgga atccggagcg gcacccatcc acccgctgag cacacgtccc
     1261 tcttcacctg tgtcgtccat ctggcggtag agccgggcga tctgcccgta ctgatcaatc
     1321 ctgtcgggcc gcttctcgct ttcggccaga tagccccgtg ccgcttcgta attcccctgg
     1381 cgcatgctga catctgccag cccgatgagc gattccgtat tgttcggcat ggcgcgcgac
     1441 cattccgcaa aagtttccat ttcctgatct atctgccctg ttttcttgta cagatgggca
     1501 agttctcgcc aggcattgct gtttttcccg gacaacgcca gactctgccg gagcagagat
     1561 tccgcctgct ggtagctgcc ccggcgtatg agtgtctgcg cctgatccac cagcgcttcc
     1621 gcctgtttct cctgcccggc tgcccctttg tcttcccgct tgtttttctc ggttcggtct
     1681 gtcggttttt tatttttact gttgtccttt tgtggagcgg cggcaccgcc ttttcccggc
     1741 agttcctgtt ttacgctgtt gtttgcgggg gactggcttg tcggagcggg atctaccgcc
     1801 ggctgctgaa cgtccccgga taccacctcc gcttccggct catcgacgct atgacctgca
     1861 gcggccgcat ttttatgcac actgtgcgca gcggcttttt ccggcctgtg ttccggcaaa
     1921 gtctcgtgag gcgctgagaa atggtgccgg aacacgagca tggacccgac agcggccagc
     1981 gcagcgaaaa gaaggatata tttcagtttt tccataaggc agatcctgtt ccggaataac
     2041 gggacggaag aggctttaaa cacagcagtt ccattataaa aagaaacagc gccgacaccc
     2101 aaaatgatac cggcgctgcc ctcaaaagac atcagaaagc ggttttattc gacaataacg
     2161 ctcttggcga ggttgcgggg ctgatccaca tcgcatcctc ggttcaccgc cacataatag
     2221 gcgaaaagct gcagcggcag cgccaccaga atgggcgaga agggttcgta gcattcgggg
     2281 atatagagca catcgtcgct gtatgcctga attgccgtgt cgccttctgt cgcaatactg
     2341 attacaagac cggcacgcgc tttgatcgac tgaatattcg ataccatttt gtcgtagatg
     2401 ttgccctttg gcgccacaca gatcaccggc atctcattgg tgaccagtgc gatggggccg
     2461 tgtttgagtt cgcctgccgg gtaggcctcc gcatggatat agctcacttc cttcagtttc
     2521 agcgagcctt ccagtgcgct gggatagttg tagtctctgc ccaggaaaaa cgcactctgg
     2581 gcatcccggt atttctgctt gcctgcaagc gctttgatcg gttcctgatt attcagcacc
     2641 agctgcattt tgtcgggcag ggtacgcagt tccgctatca tatcttttgc ctgatccttc
     2701 gtcatggtgc cgcggatccg ggccagccag atggtgagca gtgcgaaggc ggtcacctgg
     2761 gtggtatacg ccttggtgga ggcgatactc acttcagggc ctgcctgaag atagatgacg
     2821 ccgttcgatt cgcgggcgac actggagccc acggtgttga ctaccgccag aatacgggcg
     2881 ccctgacttt tcgccagtcg caccgcttcc atggtatccg ccgtttcgcc gctctgggat
     2941 acgggaatca caatggcatt tttttccaca ataggatcac ggtagcggta ttcggaggca
     3001 agatccagtt ccacggggat ttcggcaaag cgttcaatca ggtacttgcc caccatgccc
     3061 gcatgccagg aagtgccgca ggcgacaatg gtaatacgcg gacaggcttt gatatccgct
     3121 tcgctgatat taatgtccga cagattcacg tcgctggcat catcgctgat tctgccgcgc
     3181 agggtattgc ggatcacttc aggctgctgg tggatttctt tcagcatgaa atgcgggaag
     3241 ccgtcttttt ccgccgctga atcgtcccag tccacatgca ccacttcgtg ctgtaccgga
     3301 ttgccgtcca aatcctccac tttgatgcca tcacggcgca cttcacagat ctgcccgtga
     3361 tccagataca ccacatcgcg tgtatatttg atgatggcgg aaacatcgga acccatgaag
     3421 gcttcgttat caccaacgcc cacaacgacc gggctgccct gccgtgcgac aatcagcacg
     3481 tccggattgt gcctggatac gaccccgagc gcccaggcgc ctttggcaca gcgcagcgcc
     3541 tgctgcaccg ccttgaagag attgccctga tagtacttgc ggatcaggtg cgccagtgtt
     3601 tccgaatcgg tctggctgcg gaaagcgacg ccttcctgct gcagtgacgt gcgcagttcc
     3661 tgatagtttt caataatgcc gttgtggaca acagcgattt cgttcagtgc gtcaaagtgg
     3721 gggtgggcgt tggtttcatt aggtacgccg tgggttgccc agcgcgcgtg accgatgccc
     3781 agatgccccg tgaccggctg cgccgccagc ttggcttcca gattctccaa cttgcccagg
     3841 ctttttacag cggttacacc ctcatcgttc accacggcaa cgccggcgga atcgtggcct
     3901 ttgatacggt aaagccctgc caccaggact tcaacaggat tcttgccgcc aatgtatccg
     3961 actactccgg acatagactg tctcctttca gcttcgttaa tcatgaatac tgccgcagcg
     4021 ggcagacgga acttttaaaa aaaggcggct cgcacccggt agacgcaagc cgccgcatat
     4081 cgtcaagtgt gaaaataaat acggactatt tcttgtagat ctcgatcatg tcctgcagcg
     4141 tcaacggggt gtaatccgtg gcgtgaccgg cctggccgaa ggctttcatg cgtgccacat
     4201 acacttcata agcggcttcg cgggcgggct tcagatagtc gcgcgggtcg aatttcgacg
     4261 gagactccac gaagaccttg cggatggcgc ccgtaatggc gaggcggctg tccgtgtcca
     4321 cgttgatttt gcgtacgcca tgacggatac cgtcctgaat ctgctccacg ggaatgccga
     4381 aggcatcggg catgttgccg ccgtactggt tgatgatgtc gaccagttct ttcggaaccg
     4441 agctggagcc gtgcatgacc atgtgacagt tgggcatacg gtcgtggatt tcgtgaatgc
     4501 gctccatggc aagagtcggc ggaagcatct cgcctgtaac cggatctttt ctgcccgatt
     4561 tataggcgcc gtggctcgtg ccgatggcaa cggcgagggc gtccagctgt gttttggcaa
     4621 cgaagtcttc cgcctgtgcc ggatccgtca ggtgatcctg cacttccgct tcgctgagcc
     4681 ccgcaccgtg accgtcttct attccgccca gacaacccag ttcaccttca accgtcacgc
     4741 ccagaggatg ggcatattcg acgacatcgc aggtcacttt cacgttctgc tcatagctgt
     4801 tcggcgtctt gccgtcttcg gaaagagagc cgtccatcat gacggaggtg aagcccagcg
     4861 caatcgcttc tttgcaggtt gcgaggctgt tgccatggtc aagatgcatg gcgacaggaa
     4921 tatgcggata ttcttctgct gccgcttcca tcagcttttt caggtaaatc attttgctgt
     4981 atttcagcgc accgcggctc gcctgaatga tcacagggct gtgcgtatcg ttcgcagcct
     5041 gcatgatggc ctgaatctgt tccatgttgt tgacgttgaa cgcaccaaca ccgtacccgc
     5101 ccttggcggc ttcatccaaa atctggcgca tgggtactaa tggcattaca cttctctcct
     5161 tttgagttat gcagtaaggt tcagccgggc agtcgattag cgctctccgc cggatggcgc
     5221 agcgcacgaa actgctggtc gggatcgcat gccccgctca gaaatgaaat aagagaatcg
     5281 cagaaagacg atcaacttta aacaaaagtt tttatctctc tgtataaagt ctacaggatt
     5341 ttactgttgc agttcaaatg cgggtcttca ttggccctag agcggaagga aggggagagg
     5401 agacagaaaa gcgtgccggc agagataaaa agagccctct tatgccctct ctgtccactc
     5461 tctttgtgtg gcttctagct cttttaaata aaagcagtcc ttgccctgat taagattcct
     5521 gcctgcgcag gaatgacgcc ggagggtgga cgtaacggcg acgtgcgcag agacgccg
//
LOCUS       DAOPWB010000175         5573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_14517_length_5573_cov_5.235955, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000175 DAOPWB010000000
VERSION     DAOPWB010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14517_length_5573_cov_5.235955"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..983
                     /gene="folP"
                     /locus_tag="PKN92_12060"
     CDS             135..983
                     /gene="folP"
                     /locus_tag="PKN92_12060"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNZ49568.1"
                     /translation="MEYRSDTLQNPQNSATRVMGILNITPDSFYDGGLYHAENAALQH
                     LQELVDQGADIIDIGGESSRPGANLVPADEEKKRIASVLPAAVASGIPVSIDTIHAAT
                     ARFALEAGACIINDISSFRHDPAMAALAAEHECLCILMHMQGTPKTMQAAPCYKDIIS
                     DIIDFFKERIDFALKEGVAEENIWLDPGFGFGKTVAHNLELLRRFEEFQVLGFPLVLG
                     TSNKSTIGTVLNLPPDERLEGTAATVAIGIMKGVHCIRVHDVRAMSRVAKMCDAVLGR
                     DAGQTP"
     gene            1008..1733
                     /locus_tag="PKN92_12065"
     CDS             1008..1733
                     /locus_tag="PKN92_12065"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNZ49569.1"
                     /translation="MIELGVNIDHVATLRQARRGLFPDLVAAAKVCELAGAHQITVHL
                     RVDRRHIQDSDIFALKQVLLLRMNLEMAAEEEIIAIAEELRPYTVCLVPESRHEITTE
                     GGLDVAHQISKLTEVTARLHSKGILVSTFIDPEPVQIEASAAIGADAVEFHTGAYAAA
                     SGEAALRELARVREGAALASSISLDFHAGHGLSIRNLRPVARIPLLREVNIGHDIVAR
                     ALFIGLEPAVKEILTILKEESRD"
     gene            1847..2737
                     /gene="folD"
                     /locus_tag="PKN92_12070"
     CDS             1847..2737
                     /gene="folD"
                     /locus_tag="PKN92_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNZ49570.1"
                     /translation="MAITKTRIIDGKKTAAAILEELKSKTTQLKEKKIEPGLAVVLVG
                     EDPASQVYVRSKRKTCAELGIQSFSHDLPANCTEKRLLTLIHKLNDDPRVHGILVQMP
                     LPKHLNEKRVLEAIRPEKDVDGFHPVNVGRLLNGEEGFVPCTPAGCLVLLQRYGIRPE
                     GKHVVIVGRSNIVGKPLAALLIQKADNANATVTICHSRTRNIARYTREADILIAAMGV
                     PEFIKPRMVREGAVVIDVGVNRIADASKKSGTRLVGDVAFAGVSKKAKAITPVPGGVG
                     PMTIAMLMANTIRAAELLAR"
     gene            2780..3625
                     /locus_tag="PKN92_12075"
     CDS             2780..3625
                     /locus_tag="PKN92_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ49571.1"
                     /translation="MNRLLFTALSLMVCAGVYAEVPLKVMTFNVRYGTALDGENAWPH
                     RKDILIKTIRDQSPDLLGLQECLAMQADYIQAAFPEYRWFGIDRDVTGKGEMTCIFYR
                     HADLYPVESGNFWLSETPEIPASISWDSSLTRIATWARFYHKESKSWIHYYNTHLDHR
                     GEEARVQGITLISKRMAALPDGSVAILTGDFNASAEKSRAYEVAMEEGFTDAWIAAEK
                     REGPEVTFTAFKTPNLDKKDRIDWILFKGSVRVSRCETITYSENGRYPSDHFPVCATL
                     HIAVP"
     gene            complement(3657..4802)
                     /locus_tag="PKN92_12080"
     CDS             complement(3657..4802)
                     /locus_tag="PKN92_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49572.1"
                     /translation="MFFKSLKRSFTTIELEKDAGFIVTSSDVPAAYLSRIRWWNFIEA
                     WAAFAVVMAVVWCEYWMSKGATQNFRVIVGVPAILWMFIFSPVVHYRYEQCLFLHPHQ
                     LGRGLSLYFWEFRGLGNPVRYYRGYDGEGPLFLKHKKVVAGILLFMTVLYISAAFTFS
                     EEIDQRYSQYYGDSVAGKIAFIMGLLLLLNILWFAVGFPFMLRLDNFARHFRFVIAFI
                     LGSIVMILIFNLVFQFILEPLREYLEPWHHFRLRGAPARERLTALADPLAIFGQWAGY
                     VTWGWVQQLIFAGYFGVLFSRAFPVEKSRWELTKACLCTATAFTLVHLPNFWLMVFTF
                     FGGLFGTFVFLQSHNLFILGMSHGFAGSLLNKITPINFSVGASQMPK"
     gene            4993..5565
                     /locus_tag="PKN92_12085"
     CDS             4993..5565
                     /locus_tag="PKN92_12085"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNZ49573.1"
                     /translation="MGKQTVRFQCHHSGHCCRDVVCLPTPWDVLRIAMNIHRDPHDFI
                     EFLTPDEIEGVSKSDPTWLIIGDNRYIMALQRTPEEGCFFLDRKTYHCTIYEHRPLLC
                     RLYPFKLHETRTGEFSHFSLHQDVGCPRFRDGVVETAPLYDLYLQDKMHQEDYVKLVN
                     RFNALEDDSRSPEHFLDLFVEVVQAEDEEG"
BASE COUNT         1489 a         1419 c         1399 g         1266 t
ORIGIN      
        1 ggaagtaccc gatgctgaag cgcagatcac tcccccgccc ctcgaacctt tgagcgaaaa
       61 cccctgaacg ttttccaaaa tgagcatgcc gtcggctgta tgcccgcccg gcacataccg
      121 gagtccatga aacgatggaa tatcgcagcg atacgcttca gaatcctcag aactccgcga
      181 cacgcgtgat gggtatactg aatatcaccc ctgattcctt ttacgacggc ggcttgtatc
      241 atgctgaaaa cgcggcactg cagcatttgc aggaactggt ggatcagggc gctgacatca
      301 tcgacatcgg cggagaatcg agccgtcccg gagctaacct tgtacctgca gatgaggaaa
      361 agaaacgcat tgcctccgtg ctgccggcag cagtcgcttc ggggattcct gtctcaatcg
      421 ataccattca cgctgcgact gcccgttttg ctctggaggc aggcgcctgt atcatcaatg
      481 acatctcctc atttcgccac gaccccgcca tggcagctct tgctgcggag cacgagtgcc
      541 tctgtattct gatgcatatg cagggtacgc cgaaaaccat gcaggccgct ccctgttaca
      601 aagatatcat ttccgatatc attgattttt ttaaagaacg gatcgatttc gcactgaaag
      661 agggcgtagc agaagagaat atctggcttg atccgggttt tggcttcggt aaaacagtgg
      721 cgcataatct tgaactgctc cgccgatttg aggaattcca ggtactcggt tttccgcttg
      781 tcctaggcac atccaacaaa tccaccatcg ggacagtgct gaatcttccg cccgacgaaa
      841 ggcttgaagg cacagccgca acggtagcaa taggaatcat gaaaggagta cactgtattc
      901 gcgtgcatga tgtacgcgcc atgtcccgtg tcgcaaagat gtgcgatgct gtactcggac
      961 gggatgcggg gcagactcct tagcaacaga agaagggtga atccgctatg atagaactgg
     1021 gcgtcaacat cgatcatgtg gcaacattgc ggcaggcaag gcgcggtctt tttcctgatc
     1081 ttgttgcagc tgccaaggtc tgcgaactgg caggagcaca ccagataaca gtccatctcc
     1141 gtgttgatcg gcgtcatatc caggattcgg atatatttgc attaaaacag gtccttttac
     1201 tgcgtatgaa tcttgaaatg gcagccgaag aagagattat tgccattgca gaagagctcc
     1261 gcccgtatac agtctgtctg gtacctgaaa gcagacatga aattacaacc gagggcggtc
     1321 ttgatgtggc gcatcagata agcaagctga cggaagtcac cgcccgattg cacagcaaag
     1381 gcattctcgt cagcactttt attgatcctg aacctgtgca gatagaagca tctgccgcca
     1441 ttggcgctga tgcggttgag ttccatacag gtgcttatgc tgctgcttca ggcgaagctg
     1501 cattgagaga actggcacgg gtccgcgaag gtgctgcact ggcaagcagc atttctcttg
     1561 actttcatgc cgggcacgga ctcagcatac gcaacctgcg ccctgtcgcc aggatccctc
     1621 tgctgcgcga ggtgaatatc ggtcatgata tagttgcccg agctctcttt atcggccttg
     1681 aaccggcagt aaaagagatt ctcactatcc ttaaagaaga gagccgggac tgagcgactc
     1741 ttttatctcc cgaggtgctt tcagaagcag ttttttctat ttctaacgag aaacccttaa
     1801 gcgccagcta tgaaactgcc ggcgcgcaat acagaaagtg caggcaatgg caattacaaa
     1861 aacacggatt atcgatggta aaaaaacagc agcagccatt ttagaagaac tgaagtccaa
     1921 aacaacgcaa ttaaaagaaa agaaaatcga gccggggctg gcagtggtgc tggtaggtga
     1981 agatcccgcc agtcaggtct atgtgcgcag caagcgtaaa acctgcgctg aactgggtat
     2041 tcaatctttc tctcatgatt tgcctgccaa ctgtacggaa aaacggctgt tgacactgat
     2101 tcataaacta aatgacgatc cccgtgttca tggcattctg gtacagatgc cactgccgaa
     2161 acacctgaat gaaaagcgtg ttcttgaagc cattcgcccg gaaaaagacg ttgacgggtt
     2221 tcatcctgtc aatgtcggcc gcctgctgaa cggcgaagaa ggttttgtcc cctgcactcc
     2281 cgccggatgt ctggtactgc tgcagcgata cggtatccgt cctgagggca aacatgttgt
     2341 aattgtcggt agatccaaca ttgtcggcaa gccgcttgca gcacttctaa ttcaaaaagc
     2401 agacaacgcc aatgcgacag tgaccatctg ccattcgcgt acccgaaata tagcccgata
     2461 tacccgggaa gcggacattc ttatagccgc catgggcgtt cctgaattta taaaaccgcg
     2521 catggttcgc gaaggcgctg tggtaattga tgtcggcgtg aaccgcattg ccgatgccag
     2581 caaaaaaagc ggcacccgtc tggttggtga tgttgctttt gcgggagttt ccaaaaaggc
     2641 aaaagcaatt acaccggtac cgggcggtgt cggtcccatg acgattgcca tgctgatggc
     2701 aaataccatc cgggctgccg agctgctcgc ccgctaaaat ggttttcagc gccgaagcct
     2761 gtgacagaaa ggaagaaaga tgaaccgact gctttttaca gcgctgagcc ttatggtttg
     2821 cgccggagtt tatgcggaag tgccgctgaa agtaatgacc ttcaatgtcc gatatggtac
     2881 ggctctggat ggagagaatg cctggcccca tcggaaggac atactgatta aaaccattcg
     2941 ggatcagagc ccggatcttc tcggcctgca ggaatgtctt gccatgcagg ctgattacat
     3001 tcaggctgcc tttcccgaat accgctggtt cggtattgac cgagacgtca ccggcaaagg
     3061 agagatgacc tgtatctttt accgccatgc cgatttgtat cccgttgaaa gcggtaattt
     3121 ctggctctcc gaaacacctg aaataccggc ttccatttcc tgggactcaa gcctgacccg
     3181 aatcgctacc tgggcacgct tttatcataa ggaaagcaaa agctggatcc attattacaa
     3241 tactcatctg gatcaccggg gcgaagaggc gcgggttcag ggcatcactt tgatcagcaa
     3301 acgtatggct gcacttcccg acggctctgt tgccattctg accggagact ttaatgcgtc
     3361 ggcagaaaaa agcagagcct atgaagtcgc catggaagaa ggctttaccg atgcctggat
     3421 cgcagcggaa aaacgcgaag gccctgaagt cacttttact gcatttaaaa ccccgaattt
     3481 ggataaaaaa gaccggatag actggattct ttttaaggga tctgtcagag tgtcccggtg
     3541 cgagaccatt acctacagtg aaaacgggcg ctatccttcc gatcatttcc cagtatgcgc
     3601 cacgctgcat attgccgtgc cctgagcagc acgccccgtc tctaaaacaa cccctgtcac
     3661 ttcggcatct ggcttgcccc gacactgaag ttgatggggg ttattttatt cagcagcgat
     3721 cccgcaaaac cgtgggacat gccaagaata aaaagattat ggctttgcag aaacacaaaa
     3781 gtaccgaaaa gaccgccgaa aaacgtaaac accatcagcc agaaattcgg cagatggact
     3841 agcgtgaacg ctgtggcggt acacaggcat gctttcgtca attcccaacg gcttttctcc
     3901 acaggaaaag ccctgctgaa aaggacgccg aaatatccgg caaagatcaa ttgctgtacc
     3961 cagccccagg taacgtagcc tgcccattgc ccgaaaatgg cgagcgggtc tgccagcgcg
     4021 gtcagacgtt cccgtgccgg cgcacccctg agccgaaaat ggtgccaggg ctccaaatac
     4081 tcacgaagcg gctcaagaat aaactgaaag actaaattaa aaattaaaat catgacaatg
     4141 gagccaagaa taaaggcgat tacaaaacgg aagtgcctgg caaaattatc aagccttagc
     4201 atgaaaggga aaccgacagc aaaccacaga atattcagaa gcagaagaag ccccatgatg
     4261 aaagcgattt tcccggcaac gctgtcaccg tagtactggc tgtatctctg gtcaatctcc
     4321 tcagaaaagg tgaaagccgc ggatatgtaa agaaccgtca tgaacagcag aattccggca
     4381 acgacttttt tatgcttcag aaaaagcggg ccttccccgt cgtagccgcg atagtagcgg
     4441 acagggttgc ctaatcccct gaactcccaa aagtaaagac tgagcccgcg gcccagctga
     4501 tggggatgta aaaacagaca ttgctcataa cggtaatgga caacgggaga aaatatgaac
     4561 atccagagaa tggcgggaac accgacaatt acgcggaaat tctgagtagc ccctttgctc
     4621 atccagtatt cgcaccatac aacggccatc accacggcaa aggctgccca tgcctcaatg
     4681 aagttccacc accgaattct ggacagataa gcggccggca catcggagga ggtcacaata
     4741 aagccggcat ccttctccag ttcaatcgtt gtaaaactgc gttttaatga cttaaaaaac
     4801 ataactgcgt tccttgccgg aagtggtcga gggttaacat gaaaaaataa cagcagcaca
     4861 ttttagccgt tattctgttt gtctgcaaat tcaggtgacc tgccgcctac tgttcctttg
     4921 actgaagttg cactgcaaaa gttttttttg tatagttgcc ctgattttca actccaaaag
     4981 aaagtgagca taatgggaaa gcaaaccgtt cgttttcagt gtcaccattc cggacattgc
     5041 tgccgggacg ttgtctgcct gccaaccccc tgggacgttc tccgtattgc catgaatata
     5101 catcgggatc cccacgactt catagaattt ttaactcctg atgaaatcga aggagtgtca
     5161 aaatcggatc ccacctggct gattatcgga gacaatcgct acatcatggc actacagcgt
     5221 actccggaag agggctgttt ttttctggac agaaaaacct atcactgtac aatctatgag
     5281 caccgccctc ttttgtgccg gctgtatcct ttcaaactgc atgaaacgcg cactggagag
     5341 ttcagccact tcagtttgca tcaggatgtg ggctgcccga gatttcggga cggagtggtt
     5401 gaaactgcac ctctgtatga tctctacctg caggacaaaa tgcatcagga agattatgtg
     5461 aagcttgtaa ataggttcaa tgcgctggaa gacgacagcc gcagcccgga gcattttctg
     5521 gatctttttg tggaggtggt gcaggcagag gacgaggaag gctaaaaacg tat
//
LOCUS       DAOPWB010000176         5529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_14686_length_5529_cov_4.837048, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000176 DAOPWB010000000
VERSION     DAOPWB010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14686_length_5529_cov_4.837048"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKN92_12090"
     CDS             <1..509
                     /locus_tag="PKN92_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49574.1"
                     /translation="DTLILLRDQDIHSLCYTCEVFQVAHRFGATRHHFISLDIRLHHP
                     PPEEIRQRIEERQKAFRTTGKEGPFPLRLPGSRILRINWAWITPPEKEAEKQLGIYYP
                     VAQRRKVIYPALELLDGDLKRFYMAQLWKMGMTREAAFLNNRFFRLTEDDLALLQQQL
                     DGDDSRES"
     gene            complement(514..1440)
                     /gene="ftsY"
                     /locus_tag="PKN92_12095"
     CDS             complement(514..1440)
                     /gene="ftsY"
                     /locus_tag="PKN92_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNZ49575.1"
                     /translation="MALSDKGFFSRLKEGLGKTREGLFGGVKRLFFNSAQHADLYEAL
                     EETLISADVGVETTLVLIENLRRQAKEQGIKEPEKLYGLLKQEVLQLLGTEAPDISWI
                     REGEPHVMLIAGVNGSGKTTTAGKIAFRLAQEKRQVLLGAADTFRAAAAEQLTIWAER
                     SGAGIIRHQEGADPGAVAYDTVDAGAARKVDNILIDTAGRLHTKVNLMEELKKVQRVI
                     DKRQPGAPHEVLLVLDATTGQNGLQQATQFTQALQVTGIVLTKLDGTAKGGMALAIRR
                     QLGIPIKMIGVGESAEDLQPFNAQDFVNALFD"
     gene            complement(1504..2277)
                     /locus_tag="PKN92_12100"
     CDS             complement(1504..2277)
                     /locus_tag="PKN92_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49576.1"
                     /translation="MNQEVFAPPEYKEGIDADAVCGQCNTVNPEGTLLCKVCGNNLRD
                     QRLLRMTADQMLEGEAKSASNSTLLYKLLPILGLLFVLWLGVNARQLMTSMTSTQKTD
                     YELAGALAASLWNGEGKERYDDLAARIQLTRASETAADHARLTVRPMSAIKSGSYAVF
                     MRTGTEERFVGIARIAVENDSVYYAALMKNATEFRGKASLSDGLFVSRWDNSGLFSGG
                     AFYAMAGTAQIQPDGSVSLSGQSDLNTLQYNCSAYLMQD"
     gene            2476..2970
                     /gene="tadA"
                     /locus_tag="PKN92_12105"
     CDS             2476..2970
                     /gene="tadA"
                     /locus_tag="PKN92_12105"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNZ49577.1"
                     /translation="MIPTSHEYYMQQALREAEKAEAQGEVPVGCVIVLENHIIGRGYN
                     QREMLQDPTAHAEMIAITAAAEHHGSWRLEKARLYVTLEPCPMCAGAIILSRIAEVYF
                     GAYDPKAGCCGSLMNLLEDTRFNHRPAVIGGLCEEACGTVLSNFFRNIRDRQKFGKER
                     ASDN"
     gene            complement(2987..3475)
                     /gene="coaD"
                     /locus_tag="PKN92_12110"
     CDS             complement(2987..3475)
                     /gene="coaD"
                     /locus_tag="PKN92_12110"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNZ49578.1"
                     /translation="MSEKIALYAGSFDPPTLGHLDLIERASKIFDRLIVAVANNDQKT
                     ALLSVPERMELLRGMTRSMDRVEIDQFEGLTVEYAREKKAVALIRGLRVVSDFEFELS
                     LAINNQKLNPEVDTVCLMPSEPYLFLSSRQVKEIARFGGRISHYVTPEVEKSLREKLR
                     GE"
     gene            complement(3472..4656)
                     /gene="larC"
                     /locus_tag="PKN92_12115"
     CDS             complement(3472..4656)
                     /gene="larC"
                     /locus_tag="PKN92_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNZ49579.1"
                     /translation="MKVLYLDCFCGISGDMTVAALLDAGADYSLIQHAIASMNLEGLR
                     IEKDQVHKKGIQAARFQVLVDPGVKQPHRHLHHILEIIEKADMPQAVKEKAAGVFQCL
                     GEAEAEVHGTTVAKVHFHEVGALDSIVDIVAANLALHSLGVEKVISSPLVTGQGTVAC
                     DHGIMPVPAPATAVLLKGIPWHSGGVDGELVTPTGAALVRHWADSFGGADFSEVNAIG
                     HGAGTRDLPDRANVLRVFVGTAQQIPAASDHVNVVETMVDNLNPELAAVIIPALLEAG
                     ALDAWTAPVQAKKGRTGLHITALCDDAALSRVTDSLFVQSGTLGIRFRQEARRILERE
                     AVMLSTPWGDVRGKRSRLRTLIENSTPEYEDCAMLAAQHGIPVRKVYEAALADSVQGG
                     SV"
     gene            complement(4653..5420)
                     /gene="larB"
                     /locus_tag="PKN92_12120"
     CDS             complement(4653..5420)
                     /gene="larB"
                     /locus_tag="PKN92_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNZ49580.1"
                     /translation="MNEDKLRQLLADVASGAVPQAEAYARLRELPFEDLGYAKIDFHR
                     SLRKGFPETIFCQGKTPEQTASIARRMVDRGVMVLGTRCSPECFAAVKEAVPEAKYHE
                     TARLFTVLCEKTTQRPGYIAVLCAGTSDLPVAEEAAVTCEVLGNRVERIYDVGVAGLH
                     RLLEHQEQLIHASALIVCAGMEGALPSVVAGLTPAVVIAVPTSVGYGASFGGIAALLG
                     MLNSCAPGLTVVNIDNGYGAAVAASMINRTAERSQSE"
BASE COUNT         1264 a         1627 c         1389 g         1249 t
ORIGIN      
        1 ccgacacact gattcttctt cgggatcagg atattcattc tctttgctat acctgcgaag
       61 tgttccaggt cgcgcatcgc ttcggtgcca cacgacacca tttcatttca ttggatatcc
      121 ggctgcatca ccctccgcct gaagagatcc ggcagcgcat agaggaaagg cagaaagcat
      181 tccgcactac cggcaaagaa ggtcccttcc cgctgcgact cccgggaagc cgcatactgc
      241 ggattaactg ggcatggatt acgccgccgg aaaaagaagc ggaaaaacag ctgggaatct
      301 actatcctgt ggcgcagaga agaaaagtca tctaccccgc tcttgaattg ctggatggtg
      361 atctcaagcg tttttacatg gcacagctgt ggaaaatggg gatgacccgg gaagcagcct
      421 ttctgaacaa ccgctttttc cgcctgacag aggatgacct cgctttgctg cagcaacagc
      481 tcgatggtga cgacagcagg gaatcctgag ggatcagtcg aaaagggcgt tgacaaaatc
      541 ctgggcatta aaaggctgca ggtcttctgc gctttcgcca acgcctatca ttttaatggg
      601 aatgcccagc tgcctgcgga tagcgagcgc catgccgcct tttgctgtgc cgtccagttt
      661 ggtcagcaca ataccggtta cctgcagtgc ctgagtgaat tgcgtcgcct gctgaagccc
      721 gttctggccc gtggtggcat ccagaaccag aagcacttca tggggcgcac cgggctggcg
      781 tttgtcaatg acccgctgga cttttttcag ttcttccatc aggttcactt tggtatggag
      841 ccgccctgcc gtatcgatca ggatattatc cactttccgc gccgcaccgg catccactgt
      901 gtcatacgcc actgcacccg ggtcggcacc ttcctgatgg cggatgattc ccgcgccgga
      961 acgctccgcc cagatagtca gctgttcggc tgccgccgcc cggaatgtat cggcagcacc
     1021 gagcagcacc tgccgttttt cctgagccag acgaaatgct attttcccgg ctgtcgttgt
     1081 ttttccggag ccgttcacac cggcaatcag catgacatga ggttcccctt ccctgatcca
     1141 ggaaatatcg ggcgcttccg tacccagcag ctgcagtacc tcctgcttca gcagtccgta
     1201 cagtttttcc ggctctttaa tcccctgctc cttcgcctgc cgtcggaggt tttcgatgag
     1261 caccagcgtc gtttcaacgc ccacgtcggc actgatcagc gtttcctcga gtgcctcata
     1321 taaatccgca tgctgagcac tgttaaagaa gagacgcttt acgccgccga acaacccttc
     1381 ccgggtcttg ccgagtccct ctttcagacg ggagaaaaaa cctttatcag acaaagcaat
     1441 tgcctttcac aagacggcag tattgccgct tcagggagac aggactgcag atgcgccggc
     1501 acattaatcc tgcattaaat aggcgctgca gttgtattga agagtattca ggtcgctctg
     1561 tccgctgagg gacaccgacc cgtccggctg tatctgcgcc gtgccggcca tggcataaaa
     1621 ggcacctccg gaaaagagac ccgagttatc ccaccgggaa acaaaaagcc catcgctcag
     1681 agatgctttc ccccggaatt ccgttgcatt tttcatgagt gccgcatagt agacggagtc
     1741 attctccacc gcaatgcggg caatccctac aaaacgctct tccgtgcctg tccgcataaa
     1801 aacggcataa ctcccgcttt tgatggcaga catgggccgt acagtcagcc gtgcatgatc
     1861 ggcagctgtt tcgcttgccc tggtcagctg aatgcgtgca gccagatcat catagcgttc
     1921 tttgccttcg ccgttccaaa ggctcgccgc cagagcacct gccagctcgt aatcggtttt
     1981 ctgggtggac gtcatagacg tcattaattg tcgtgcattg actccgagcc aaaggacaaa
     2041 aagcagcccc aggatgggaa gcagtttgta caggagtgtc gagttggagg ctgattttgc
     2101 ttctccctcc agcatctgat ccgctgtcat gcgtagcaac cgctgatcac gcaggttgtt
     2161 gccgcagact ttacacaaaa gggtaccttc aggattgacc gtgttgcact gaccgcaaac
     2221 ggcatccgca tcaatacctt ctttatattc aggcggtgca aaaacttcct gattcatcta
     2281 aggtatactc tttactcttt tggttagcag tgttaaaaac aatacacgta actctttaat
     2341 agtatgtaca atttgtcaac aaatttcaag ccttacatct tttttcgatc gacatccatc
     2401 cggacagaag gaaaaagcag cgctgcggct gcctgctgtt tgactttttc ctgcctactg
     2461 agacacaatg ccgccatgat tcctacttcc catgaatatt atatgcagca ggcattgcgc
     2521 gaagcagaaa aagcagaggc gcagggtgag gtgccggtcg gatgcgtcat tgtgctggaa
     2581 aatcacatca tcggcagagg atacaatcag cgggaaatgc tgcaggatcc gacagcacat
     2641 gctgaaatga tcgccattac agccgcagcg gagcaccacg gaagctggcg tttggaaaaa
     2701 gcaaggcttt atgtgaccct cgaaccctgc cccatgtgtg ccggagccat tatcctgtcc
     2761 cgaatcgctg aagtatattt cggagcctac gaccctaaag cgggctgctg cggctctctt
     2821 atgaacctgc tggaagacac aaggttcaat catcgccctg ccgtcatcgg cggcctctgc
     2881 gaagaggcct gcggcacagt cctcagcaat tttttccgga atatccgtga caggcagaaa
     2941 ttcggaaaag aaagagcctc agacaactga gccgatccgc acttttttac tcaccccgca
     3001 gcttttcacg aagacttttt tccacttccg gcgtcacata atggctgatc ctgccaccga
     3061 accgtgcgat ctccttcacc tgccgtgaac tcagaaagag ataaggctca ctgggcatga
     3121 ggcagaccgt atccacctcc ggattaagtt tctgattgtt gatcgccaga gacagctcaa
     3181 attcaaagtc ggacacgacg cggagtcccc ggatcaaagc aaccgctttt ttttcccggg
     3241 catattccac cgtaagcccc tcgaactggt cgatctccac tctgtccatt gatcgggtca
     3301 tgccgcggag caattccatg cgctccggca cagagagcag cgcagttttc tgatcattat
     3361 tcgctacggc aacaatcagc cgatcgaaga ttttggaagc ccgctcaatc agatccagat
     3421 gcccgagtgt gggaggatca aaacttcccg cataaagcgc aatcttttca ctcatacgct
     3481 gccaccttgt acgctgtctg ccagcgccgc ctcatacact tttcgcacag gaataccatg
     3541 ctgcgccgca agcatcgcac aatcttcgta ctccggggta ctgttttcga tcagagtccg
     3601 gagcctgctt cttttaccgc gcacatcacc ccagggtgtg gaaagcatca cagcttccct
     3661 ctccagaatc cgccgtgctt cctggcgaaa acgaatgccc aacgtgccgg actgaacaaa
     3721 aagactgtct gtaacccggg acagagcagc atcgtcacaa agtgctgtaa tgtgaagccc
     3781 ggtgcgccct tttttcgcct gcacaggagc tgtccaggca tcaagggcac ccgcttccag
     3841 cagggcgggg ataatgactg ctgccagctc cgggttcaga ttatccacca tcgtttccac
     3901 cacattgaca tgatcagatg ctgccggtat ctgctgtgcc gtaccgacaa acacacggag
     3961 cacattggcg cggtcgggaa gatcacgggt gccggcacca tgaccgatgg cgttcacttc
     4021 tgaaaagtcg gcgccgccga aactgtccgc ccaatgacgt accagtgccg ctccggtagg
     4081 cgttaccagt tccccgtcaa caccgccgga atgccaggga atgcctttaa gcagtacagc
     4141 ggtggcaggt gccggcacag gcataatgcc gtggtcgcac gccactgttc cctgcccggt
     4201 caccagcggt gaggaaatca ccttttcaac acccaaagaa tggagcgcca gattggctgc
     4261 gacgatgtcc acaatggaat ccaatgcacc cacctcatga aaatgcactt ttgcaaccgt
     4321 tgtgccgtgt acttccgctt cggcttcgcc aagacactga aaaacgcctg ccgctttttc
     4381 ttttacagcc tgaggcatat ctgccttttc tataatctca aggatgtgat ggagatgccg
     4441 gtgcggctgc ttcacccccg gatccaccag cacctgaaag cgggcggctt ggatcccctt
     4501 cttgtgcact tgatcttttt caatgcgaag cccctcgaga ttcatagagg cgatggcatg
     4561 ctgaatcaga gaataatctg ctccggcatc cagcagcgca gccacggtca tgtcgccgct
     4621 gataccgcaa aaacaatcaa gatataagac tttcattcgg attgactcct ctccgccgtc
     4681 cggttgatca tggatgcagc aactgccgca ccgtatccgt tgtctatatt cactacagtc
     4741 agcccgggtg cacaggaatt caacatgccc agaagcgctg ctattcctcc gaaactggcg
     4801 ccatagccaa cactcgtcgg aaccgcaatc accacggccg gggtcaaccc cgccacaacg
     4861 ctgggtagtg ctccttccat accggcacat acaatcagcg cggaagcatg gatcagctgt
     4921 tcctgatgtt ccagcagcct gtgcagtccc gcaacgccca catcataaat gcgttccacc
     4981 cggttgccca gaacttcgca ggtcaccgcc gcttcttccg ccacaggcag gtcgctggtg
     5041 cctgcgcaaa gtacggctat gtagccgggg cgctgtgtcg tcttttcgca aagtaccgta
     5101 aagagccggg ctgtctcgtg atatttcgcc tcaggcaccg cttccttcac ggcagcaaag
     5161 cattcagggc tgcaccgtgt gccaagcacc ataacgcccc ggtcgaccat gcgccgggcg
     5221 atggacgctg tctgttcagg cgttttgccc tgacagaaaa tagtctcggg aaagcccttg
     5281 cgcagcgacc ggtgaaaatc aatcttggca tagcccagat cttcaaaagg gagctcgcgg
     5341 agcctggcat aagcctctgc ctgaggcact gcgccggaag ccacatcggc gagaagttgt
     5401 cgcagtttgt cttcgttcat ggcagtactt tcagataaac ccggaactct ggattgactc
     5461 cgggggtatc gtggaagtat agcacaaggt cggaagttgc cgcattcgca ggtgtgacag
     5521 ggaaaggca
//
LOCUS       DAOPWB010000177         5496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_14817_length_5496_cov_5.151259, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000177 DAOPWB010000000
VERSION     DAOPWB010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14817_length_5496_cov_5.151259"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="PKN92_12125"
     CDS             complement(<1..1472)
                     /locus_tag="PKN92_12125"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNZ49581.1"
                     /translation="MKLKQIFFLSVLLLLLCALYVFMGWKERRHAEQALEAGKVFSFE
                     APAIRELTFHHRGPELVIRGERSAGGLWEITEPNASIAPFQIMWNRVAQHIAALINER
                     SLPADITDLAQYGLEPPVLTLNGTLDSGESFALQLGDIDILERHHYARLNGGNVFLIT
                     TEAFFELNRSLLELRHRYLVADREEKIRELEFARIWSGAPAEKEEGEKESGEERTYPE
                     VGAESVLIRVERGTDDAPWKLTSPVDALANYEKVEALSETLQYAVCTEFIDAPENLAD
                     YGLDPPKARLSVKDRNSTDWRTLWLGDIDTTPGKEGLFVRVEGEQSIMVADAQLAGLL
                     PRSPLEWRDLRLITRRISDIRKLIYDAPDSRFTLGKDETGRWKLLEPEMDNVNDTAVN
                     AFLQFIKTVAGDAAVDSPDAEQALNNADRTITIEREDGTSSSLALAPHPSDENLWWAR
                     QDSGGIIQLTGVGVKMLLCDAETFRSREILRLQKDKVRALT"
     gene            complement(1469..3145)
                     /locus_tag="PKN92_12130"
     CDS             complement(1469..3145)
                     /locus_tag="PKN92_12130"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNZ49582.1"
                     /translation="MTMIRQGRSFFGIAALLCLVLFLNILVWKQAFFSPAVLLPLGLS
                     LLFFIGWLICLAAALAEHRAIEGRTAGGLGAAVSTILFLGICIVLYLFLSAWKVSWDL
                     TEEGRRSLSTQTVQVLQSMNTEVEVLCFFLQVEEELVTIARDKTLRFLDACREHTSLM
                     KVEMLDPTIDRARLEAMNITHASVQGTIVLRAGDRQRVILLTGGSPRLEERDFTNALI
                     NVLRKSEPKVYFLTGHRERDILEEDEQSGANIFGKLLQGEAYQVDRLGIKISDPEIPM
                     DADVLVINNPVMDFHPVEIEALNQFMDRGGRLLLLMDPWRAPPAGTGEEKLRPFLEKR
                     FGIRVGSDIVITNASDNIWRAELTVDDEPFEMVEEGFMEFRGSFNRNHPITGKFDQTL
                     LLQAMRSVTPAESVPAGVALTELVRTTPDFWAESDAEKLLQTGQAQQDAEEKAGPVSL
                     AVAGIAPADKSGNPKGKGDTRIVVVGDSDFAANANIIIPGNLNFLLNIFAWLTESEDL
                     IAMRPTGREALPLILTAAQQRTLAWVSILFTVQTIVLAGIAVHLVRRKFR"
     gene            complement(3142..3972)
                     /locus_tag="PKN92_12135"
     CDS             complement(3142..3972)
                     /locus_tag="PKN92_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNZ49583.1"
                     /translation="MTALLSIARREFSSYFTTSIGYVVLGIFAAISGVAFTLSLLGYA
                     KMSLSPTAYGYTSTPDFAETFLSPYLVFCGTLIMFLAPLITMRLVAEEKHRGTMELLH
                     TWPLRDRDIIFGKYLAALGIVMLMLLIIGIHLILVGVMSPMEPAVLVFGMLTVLLMGA
                     AFISVGLFISSISRNQITSGTITFGLSLFLFIAGSLSEQLPEGNPAPAAWPGILQSAI
                     GHVYALLRGLTLELPVDAHAREMALGVVHPRDIGYYLLFSAFFLFLTFRAFESRNWRA
                     "
     gene            complement(3969..4706)
                     /locus_tag="PKN92_12140"
     CDS             complement(3969..4706)
                     /locus_tag="PKN92_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ49584.1"
                     /translation="MIKADNLSKSFGSITALSDASFQVEKGCIAGFLGPNGAGKSTAM
                     RILTGFFPASSGRAYIDGLEVHEHPLEIKRRIGYLPEHVPLYEEMSVTAFLSHVCRVK
                     GIGRKALRGERDRIMEHCGLTHMARRLVGHLSKGYRQRVGLAQALAGNPPVLILDEPT
                     VGLDPAQIVEIRNLIRTLGKDHTVLLSTHILPEVAMTCDQVIIINRGRIVTQSPMEQL
                     TLSGSRSLEEVFMSAISSEQLEESPSS"
     gene            complement(4897..>5496)
                     /locus_tag="PKN92_12145"
     CDS             complement(4897..>5496)
                     /locus_tag="PKN92_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49585.1"
                     /translation="QHSFELVNPIPLPLSQLAPVPELFTRHSPIHGQPHVSRVIIHTF
                     ILTKALGLEDYALKAWAAAYIHDLGRVDDGVSPEHGLYAVGKVQDNPELKTLFEGCGV
                     KEKDWTEIFFAVTHHCLEEVPLDHPCRTLTALLKDADGLDRVRLGGLDPSFLRFPLSV
                     SLIPYAWRLFRNTRDLAKPGQDYFEILWPVARQLLQKET"
BASE COUNT         1274 a         1526 c         1514 g         1182 t
ORIGIN      
        1 gtcagtgcgc gcaccttgtc tttctgcagg cggagtattt cccgggaacg gaaggtttcg
       61 gcatcgcata ggagcatttt aacgcccacg cctgtgagtt gaattattcc gccgctgtcc
      121 tgtcgtgccc accacagatt ttcatcggaa ggatggggtg ccagtgccag cgaggagctc
      181 gtgccatctt cacgctcaat ggtgatcgtt ctgtcggcat tattgagagc ctgctccgca
      241 tccggggagt cgacggcagc atctccggca acggttttga tgaattgaag aaaagcattc
      301 accgctgtat cattcacatt gtccatttcc ggttcgagga gtttccaccg gcctgtttcg
      361 tccttgccca gcgtgaaccg gctgtccggt gcgtcgtaaa tgagtttgcg gatatcgctg
      421 attctccggg tgatcaggcg cagatcccgc cactccagcg ggctgcgtgg caggagccct
      481 gccagttggg catcggcaac catgatggac tgctcgcctt ccacacggac aaaaaggcct
      541 tctttccccg gggtggtgtc tatatcaccc agccagaggg tacgccagtc cgtgctgttc
      601 ctgtctttca cggacagacg cgctttcggc ggatcgagtc cgtaatcggc gagattttca
      661 ggtgcgtcga taaactcggt acatacggca tactgcagtg tttcgctgag tgcctccact
      721 ttttcataat tggcaagggc atcaacggga gaagtcagct tccagggggc atcatcagtg
      781 ccgcgctcca cacgaatcag gacagattct gcgccgactt cagggtaggt gcgctcttca
      841 ccactttctt tttcgccttc ttccttttct gccggggcac ccgaccagat gcgggcaaac
      901 tccagttccc ggatcttttc ctcacggtcg gccaccagat accggtggcg cagttccaga
      961 agggaacggt ttaattcaaa aaatgcttct gtggtgatta gaaaaacatt tcctccattg
     1021 agccgggcgt aatggtgccg ctccagaata tcgatgtctc ccagttgcag ggcgaaagac
     1081 tcgcctgaat ccagcgtgcc attcagggtg aggacgggtg gctccagacc gtattgcgca
     1141 agatcggtga tatctgcagg cagggaacgc tcattgatga gagcggcaat atgctgcgcc
     1201 actctgttcc acatgatctg aaagggggca atggaagcat tgggctctgt gatctcccaa
     1261 agacctccgg ctgaccgttc tccccggatg acgagctccg gaccccgatg atgaaaggtg
     1321 agttcccgga tcgccggggc ttcaaacgaa aaaaccttgc ccgcttccag tgcctgttcc
     1381 gcatggcgcc gctctttcca tcccataaag acgtaaagag cgcaaagcag aaggagcagc
     1441 accgacagga aaaagatttg cttcagcttc aacggaattt cctccgtacc agatgaacag
     1501 caataccggc aagtacgatg gtctgcactg taaagagaat ggatacccac gccagcgtgc
     1561 gctgctgcgc tgcagtgaga atgagtggaa gcgcctctct gcctgtcggt ctcatggcga
     1621 tcagatcttc actttccgtc agccacgcaa agatgttcag cagaaaatta agattgcccg
     1681 gaatgatgat attggcgttg gcagcaaaat ctgaatcgcc caccacaaca atacgggtat
     1741 ctccctttcc tttgggattg cccgatttgt ccgccggcgc aatgcctgcc actgccagag
     1801 aaacaggccc tgctttttct tccgcatcct gctgcgcctg accggtctgc aacagcttct
     1861 ccgcatcgct ctccgcccag aagtcgggtg tggtgcgcac caattctgtg agcgccacgc
     1921 cagcaggcac tgattccgcc ggggtgacgg aacgcatcgc ctgcagcagc agggtctgat
     1981 caaattttcc ggtaatggga tggtttctgt tgaaagagcc ccggaattcc ataaatcctt
     2041 cttccaccat ttcgaagggc tcgtcatcca ctgtcagctc cgcacgccaa atgttgtcgg
     2101 aagcattggt aatcacaata tcgctgccta cccggatgcc gaagcgtttt tccaggaaag
     2161 gcctcaattt ttcttcacct gttcctgcgg gcggggcgcg ccaggggtcc atgagcagga
     2221 gcagtcgccc gccccgatcc ataaattggt tcagtgcctc aatttcaacc ggatggaaat
     2281 ccatgaccgg attgttgatc acaagtacat cggcatccat ggggatttca ggatcgctga
     2341 tttttatgcc cagccgatcc acctgatagg cttctccctg cagcagtttt ccgaaaatat
     2401 tggcgcccga ctgctcatcc tcttccagaa tatcccgctc gcggtggccc gtcaaaaaat
     2461 agacttttgg ctcacttttg cgcagcacat tgatcaacgc attggtaaag tcacgctctt
     2521 ccagccgggg gctgccgccc gtgagaagaa tcacccgctg ccggtctccg gcacgaagca
     2581 cgatggttcc ctgcacggag gcatgggtga tgttcatggc ttccagccgc gcacggtcta
     2641 tggtcggatc aagcatttcc actttcatga gggacgtgtg ctctctgcag gcatcaagaa
     2701 agcgcagcgt tttatcccgc gcgatggtga ccagttcctc ttccacctgc agaaaaaaac
     2761 aaagtacttc cacttctgta ttcatgctct ggagcacctg cacggtctgg gtggaaaggc
     2821 tgcgcctccc ttcttccgtc agatcccagg agactttcca ggcactcaga aaaagataca
     2881 gaacaataca gatgccgaga aaaaggatgg tgctcacagc agcgccgagc ccgcccgccg
     2941 tgcgcccttc aatcgcacga tgctccgcca gcgcagcagc caggcagata agccagccga
     3001 tgaaaaaaag aagactcagc cccagcggca gcagaacggc cggagaaaag aatgcctgtt
     3061 tccagacgag aatattcagg aaaagcacaa gacataaaag cgcggcaatg ccgaagaaac
     3121 tgcgtccctg tctgatcatg gtcatgctct ccagttccgg ctttcgaaag ctctgaaagt
     3181 caggaaaaga aagaaggcgc tgaagaggag gtaatagccg atatcccggg gatggacgac
     3241 accaagcgcc atttcacggg cgtgcgcatc cacgggcagc tccagggtca ggccgcgcag
     3301 aagcgcatac acgtgaccga tagcggactg cagtattccg ggccacgcag ccggtgccgg
     3361 gttgccttcg ggaagctgtt cactgagact tcctgcaata aacagaaaga ggctgagccc
     3421 gaaggtgatg gttcccgaag tgatctggtt gcgggaaatg gaggagatga acagacccac
     3481 actgatgaaa gcggctccca tcagcagcac ggtcagcatg ccgaaaacaa gcactgccgg
     3541 ttccatgggc gacatgacgc ctaccagaat cagatgaatg ccgataatca gcagcatgag
     3601 catcacaatc ccgagggcgg cgagatactt gccgaaaata atatcccggt cacgaagcgg
     3661 ccacgtgtga agcagttcca tggtgccccg gtgcttttct tccgccacaa ggcgcatggt
     3721 gatcagggga gcaagaaaca tgatcagcgt tccgcagaaa acgagatagg ggctcagaaa
     3781 ggtctcggca aagtcagggg tcgatgtata gccgtaggca gtgggggaga gggacatttt
     3841 tgcatagcca agcagggaga gcgtgaaggc aacgcctgat atggcggcaa aaatacccag
     3901 caccacatac ccgatggaag tggtgaaata gcttgaaaat tcgcggcggg caatactcag
     3961 caaagcagtc atgatgatgg tgactcctcg agctgctccg aactgatggc ggacataaac
     4021 acttcttcaa ggctccggct gccggacagg gtgagctgtt ccatgggact ttgcgtcaca
     4081 atccggccgc ggttgatgat aatgacctga tcgcaggtca tggcgacttc cggcagaata
     4141 tgggtactca gcagcaccgt atgatccttt cccagcgtac ggatcaggtt gcggatctcg
     4201 acgatctgtg caggatccag cccgacggtc ggctcgtcca gaatgagaac aggcggattg
     4261 cccgccagcg cctgcgccag accgacccgc tgccggtacc ctttggacag gtgacccacc
     4321 agtctgcgtg ccatatgagt caggccgcaa tgctccatga tccggtcacg ctcgccgcgc
     4381 agcgcttttc tgccgattcc tttgacccgg cacacatgac tcaggaaagc ggttacggac
     4441 atttcctcat aaagcggcac atgctccggc aggtagccga tgcgccgttt aatttccagc
     4501 ggatgttcgt gcacttccag cccgtcgatg tatgcccggc cactgcttgc cgggaaaaag
     4561 cctgtgagga tgcgcatggc tgtgcttttg ccggcgccgt taggtcccag aaatccggca
     4621 atgcagccct tttccacctg aaaagaggcg tcgctcagtg ccgtgatgga accgaaagat
     4681 ttacttagat tatctgcctt gatcatggaa aaccctgtgt gtcggtacag tcgggaaata
     4741 gtgactcggt atcagtattc taccttattg acatggcata ccggaaagaa tgccacccga
     4801 tgcggacgga tgagatatgt gcccgcactt ctttatgcct gtaaccagta gggtacatta
     4861 acaggcgtcg tttttcaaga accggaggaa tgcggttcag gtttctttct gcagcagctg
     4921 ccgggcgacg ggccagagta tttcaaaata atcttgcccg ggctttgcca gatccctggt
     4981 attcctgaaa agacgccagg cgtagggaat caggctcacg gaaaggggaa agcgcagaaa
     5041 agaaggatcg agaccgccca gacgaacccg atccagtccg tcggcatctt tcagcagtgc
     5101 ggtaagtgtc cggcagggat gatccaaggg cacttcctcc agacagtggt gggtcaccgc
     5161 aaagaagatt tccgtccagt ctttttcttt gacgccgcaa ccctcaaaca gggtcttcag
     5221 ttccggatta tcctgcactt ttcccaccgc atacagccca tgttcaggcg aaacaccatc
     5281 atccactctg ccgaggtcat gtatataggc ggccgcccag gctttcaggg catagtcttc
     5341 cagaccgagt gcttttgtaa ggataaaggt atggatgatg acccgggata catggggctg
     5401 gccgtgtata gggctgtgcc gggtgaaaag ctcaggcacc ggtgccagct gcgaaagagg
     5461 cagcggtatc ggattgacca gttcaaaaga atgttg
//
LOCUS       DAOPWB010000178         5409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_15135_length_5409_cov_4.522600, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000178 DAOPWB010000000
VERSION     DAOPWB010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15135_length_5409_cov_4.522600"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKN92_12150"
     CDS             <1..687
                     /locus_tag="PKN92_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan phosphorylase"
                     /protein_id="HNZ49586.1"
                     /translation="EAEGLGENGWSIGKGEIYDSLEDQDTVESEALYEILEQDVVPMF
                     YNRGRDNLPREWIGRMKNAIRTIAPMFNTYRMVLEYGDRFYIPNCRRRHRLFQNERKA
                     VYELAAWKQKVRTFWPQVQILDVANGNAENLYFGDTLEVTAKIHLGNLSEKDVKVEIY
                     HGALKPDGALEAAAKERMVWVKALDGDVHLFKGRARCDRTGALGISVRVLPSHPDLIH
                     EHETGLITWA"
     gene            977..3283
                     /locus_tag="PKN92_12155"
     CDS             977..3283
                     /locus_tag="PKN92_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNZ49587.1"
                     /translation="MNISVRRFKQRMAGLKNRQTGLAAGLFLALILLGGCAHSPGRIA
                     QFDPAPPGEVDVSELESCLPAAVSDLLRQAEEELKLGNIAHEKGDQDTARTHYRKMFS
                     LLHEAELDPSLNYASRELFERVLTAHLNQSHLYHESGSCLGKSGSEVYSDLIIPTPLP
                     PQVIAEIKELQNSYAPTFQRGLNRSASYMPHLRNMFRDAGLPEELVWLAMVESMFQTK
                     VISPAGAGGMWQFMRGTARHYALHMDQYVDERFDWHRSTEAAICYLKNLHNFFNGDWA
                     LAVTAYNMGEGGLDRTLTACGGERDFWALLNNDSPNNKMKEESKKYFPRLLAYIIVAN
                     SPEQYGFSSPTDPPLNIRRVPVTGCYRLAALEEATGISKGTLAALNPELLREVTPGTG
                     QYHLTVPGDRHEEILAALSTIKPGPVPAPAAPVLAHAGGTTHRVKRGESVSQIARHYG
                     VSESALMKANKMKSAHSLKANQTLHIPGAAAAARADSGTSSPQAAPEKTTAVASAPPA
                     AKAAPAEKTVDIQYTVKKGDTLSKIAQAHKVPMAQLQKWNNIPASKVVQIGQVLKVGE
                     GKQAAVQPAARANEQFHLVKAGEYPAIIAELYDIPLKDLLAWNNLNGNSIIRAGDKLI
                     VSGQVSSPAAATAAAEKPKAPAPKEITHKVAKGETAGSIAKKHNVSLEDLLAWNKLTT
                     KSVLRVGQSLSIKTTADAAVGRDRGKDLTLSQAAAEQKLVHKVTAGQNPTSIARHYGV
                     TVNDLFKWNNWKESHVLRVGDEVSIYKK"
     gene            complement(3340..4434)
                     /locus_tag="PKN92_12160"
     CDS             complement(3340..4434)
                     /locus_tag="PKN92_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNZ49588.1"
                     /translation="MKQRCTIYDIAHKSGVSTKTVSKVINNLPGVKESTRQKVWQIVR
                     ETGYYPHHGARSLRGQVRNCVGVTLAPPPHQVPLSEDFLLWLFAKLSEVFASRGAYLS
                     FDLAPRSQEILGSDYGRGVWEQVYDACLIVGPLPVQDSVLQRIHMSGIPYLALGQTDA
                     FPEGSCASVNFYEGALQSTRFLLERGHKRIALLTTYEGYQAGVDRIRGYRDALAEKNI
                     PFNPSLVAYGDPASGNLEEELTALLSQPGVTALIESTAFEDGELIRSAAEKSGHGAGD
                     SLACVVWTYSRDEAVLPEATAHLWLPVLECSVEGIHELALWMEGARSGPIQVLHSPVL
                     SDAPRPRAMSKPTRIGERLGFHVKNIRKRP"
     gene            complement(4588..>5409)
                     /locus_tag="PKN92_12165"
     CDS             complement(4588..>5409)
                     /locus_tag="PKN92_12165"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNZ49589.1"
                     /translation="EKLFHDVRLSKDDTISCASCHALDLGGTDQKKFSPGVDGAMGDI
                     NSPTVYNSVFQFAQFWDGRATDLADQADGPVNNPVEMASNWDQVIGKLTQDDVFTEEF
                     LAVYPDGYSKANFTDAIAEYEKTLLTPAPFDAFLKGDASALSAEQKAGFQHFQDLGCY
                     TCHNGVILGGKSYEPMGLRRDYFRDRGNVVKPDYGRFNHTGDEFDRFRLKTPTLRNIA
                     VSFPYFHDGSANTLEEAVETMVKYQVGKKPQASQTAEITAFLHSLTGAYQGKMLQ"
BASE COUNT         1352 a         1489 c         1411 g         1157 t
ORIGIN      
        1 gaagcggaag gactgggtga aaacggctgg agtatcggca agggagaaat ttacgacagc
       61 ctcgaagacc aggacaccgt tgaaagtgag gcgctttacg agatcctcga gcaggatgtc
      121 gtgcccatgt tctataaccg cggacgggat aaccttcccc gggaatggat cgggcgcatg
      181 aaaaacgcca tccgcaccat tgcacccatg ttcaatacct atcgtatggt tcttgaatat
      241 ggcgatcgct tctatatccc gaattgcaga cgccgccacc gcctctttca gaacgaacgg
      301 aaagccgtgt atgagctcgc ggcatggaaa caaaaagtac ggactttctg gcctcaggtg
      361 cagattctgg atgtggcaaa cggcaatgca gaaaatctgt acttcggaga tacactggaa
      421 gtgacagcga agatacatct cggaaatctt tcggaaaagg atgtgaaagt agagatttat
      481 cacggtgcgc tgaagccgga tggcgcactg gaggcagccg ccaaggaacg gatggtctgg
      541 gtaaaagcgc tggacggcga tgtacatctc ttcaaaggac gtgcgcgctg cgaccgcacc
      601 ggtgcactgg gtatttccgt gagagtgctg ccaagccacc ctgatctcat ccacgagcac
      661 gaaaccgggc tgattacctg ggcataagca gcggatttta tagcgaaact gccggcattc
      721 tgacgtgtcg gcagtttcct tctttatgga aaaccacctt atgttggcgc aaatggcttg
      781 acataccaca agatatagtt tacactatga aatatgtgaa cggatgtctt gtgtacagtt
      841 ttctatcata atctattcaa tcgccggaaa aaccggagtc tgctgcttcc ggggaaatga
      901 ttggtcttta tctgaacgta tcagaagtat atttgtgcct tgcatctgat acgcaacgag
      961 aggagcaaga tcttcagtga atatttcagt cagacgcttt aagcaaagaa tggcggggtt
     1021 aaaaaacaga caaacgggac tggcggcggg gctttttctt gcgctgatac tgctgggagg
     1081 ctgtgcccat tcccccggac gtattgctca gtttgaccct gcccctccgg gcgaagtcga
     1141 cgtaagtgaa ctggaaagct gccttcctgc ggctgtgtct gacttgctgc gtcaggcgga
     1201 agaagaactg aaactgggca atatcgccca cgaaaaaggg gatcaggaca cagcgcgcac
     1261 ccactaccgg aaaatgtttt cgcttcttca tgaggcggag cttgatccct ctctgaacta
     1321 cgcatctcgc gaactttttg aacgcgtcct tacagcgcac cttaaccaga gccatctgta
     1381 tcacgaatcg ggcagctgcc tcggaaagtc cggttccgaa gtttacagcg atctgatcat
     1441 cccgacccct ctgccgcctc aggttattgc cgagattaaa gaactgcaga acagctacgc
     1501 tccgaccttt cagcgcggac tgaaccgcag cgcctcttac atgccccatc tgagaaacat
     1561 gttcagggat gccggactgc ccgaagagct tgtctggctt gccatggttg aaagcatgtt
     1621 tcagacaaaa gtcatttctc ccgccggagc aggcggtatg tggcagttca tgcgcggtac
     1681 tgcccgccac tacgcactgc acatggatca gtatgtggat gagcgctttg actggcatcg
     1741 ctctacggaa gcagccattt gctacctgaa aaacctgcac aactttttta acggcgactg
     1801 ggcgctggcg gtgacggcgt acaatatggg tgaaggaggt ctggaccgca ccctcactgc
     1861 gtgcggcggt gaacgggatt tctgggcgct tctgaacaac gactcgccga acaataaaat
     1921 gaaagaggag tcaaaaaagt actttccccg gctccttgcc tatatcattg tcgccaacag
     1981 cccggaacag tatggttttt cttcaccgac cgacccgccg ctgaatatcc ggcgtgtgcc
     2041 tgtaacagga tgttaccgcc tcgccgcact ggaagaagcc accggcatat ccaaaggcac
     2101 tttagccgca ctgaatccgg agctgcttcg tgaagtgaca ccgggaaccg gtcagtatca
     2161 cctgaccgta cccggcgaca ggcatgaaga gattcttgcc gctttaagca ccatcaagcc
     2221 cggtcccgtg cctgcacctg ccgcgcctgt acttgcccat gccggcggta caacacaccg
     2281 tgtcaaacgg ggtgaaagcg tttcccagat tgcccggcac tacggggtca gcgaatctgc
     2341 gctgatgaag gcgaataaaa tgaagtccgc tcactccctg aaagccaacc agaccctgca
     2401 tattccgggc gcagcggctg cagctagggc ggactccggc acttcatctc cccaggctgc
     2461 ccctgaaaaa acaacggccg tcgcctctgc cccgcctgct gccaaagcgg caccggcaga
     2521 aaaaacggtc gatattcaat acaccgtcaa aaaaggcgac acgctgagta aaatcgcgca
     2581 ggcgcataaa gtacccatgg cgcagctgca gaaatggaat aatattcccg ccagtaaagt
     2641 ggttcagatc gggcaggttc tgaaagtcgg ggaaggcaaa caggctgctg tgcagcctgc
     2701 cgcccgcgcc aatgaacaat ttcatctggt taaggccgga gaataccctg ccatcatcgc
     2761 agagctctac gatataccgt taaaagatct ccttgcctgg aacaatctca acggaaattc
     2821 catcattcgg gcaggagaca aactgattgt ttccggacag gtctcctcac ccgctgctgc
     2881 cactgcagcc gctgaaaaac cgaaggcacc ggcacccaaa gagataacgc ataaagtcgc
     2941 caaaggagaa acagccggaa gcattgctaa aaaacataat gtatctctgg aagacctgct
     3001 tgcctggaat aagctgacca ccaaatcagt cctgcgcgtc ggtcagagcc tttccattaa
     3061 aacgaccgca gacgctgctg tcggacggga tcgcggcaaa gacctcacgc tttcacaggc
     3121 agcggctgag cagaaactgg tgcataaagt caccgcaggg caaaacccta ccagtatcgc
     3181 ccgtcattat ggcgtgaccg tgaatgacct gttcaagtgg aacaattgga aggaaagcca
     3241 cgtgctgcgc gtgggcgatg aagtgtccat ttacaagaag taaaactaac tgatccaagg
     3301 tgatgacagc ccggccatgc ggtctgaaca gtgccggaat cacggacgct tccggatatt
     3361 tttcacgtga aaaccaagcc gctctccgat gcgtgtgggc ttggacatgg cacgggggcg
     3421 cggcgcatca ctaagaaccg ggctgtgcag cacctgtatg ggtccgcttc gggcgccttc
     3481 catccacaac gccagttcat gaataccttc cacagagcat tccaggaccg gcagccataa
     3541 atgggctgtc gcctcgggca gcaccgcctc gtcacggcta taggtccata caacacaggc
     3601 aagtgaatcc ccggcgccat gcccgctttt ttctgccgct gaccgtatca gctcaccatc
     3661 ctcgaaagct gtcgactcaa tgagcgccgt cacaccgggc tgtgacagaa gtgccgtcag
     3721 ctcttcttcc aaattaccgg atgccggatc accgtaagca accagcgacg gattaaaggg
     3781 aatatttttt tctgccagcg catcacggta gccccggatc cggtcaacgc cagcctgata
     3841 tccttcatag gtggtcagca gcgcaatgcg tttgtgtccc cgctccagaa gaaagcgggt
     3901 gctctgcaat gcgccctcat aaaaattcac agaggcgcag ctgccttcgg gaaaagcatc
     3961 ggtctggccg agtgccaggt aagggatccc ggacatatga atacgctgaa gcacactgtc
     4021 ctgtaccgga agaggaccta caatcaggca ggcatcatat acctgctccc acacaccgcg
     4081 cccgtaatca ctgcccagga tttcctgaga ccgtggcgcc agatcaaaac ttaaataagc
     4141 accccgggaa gcaaatacct cagataattt tgcaaaaagc cagagcagaa agtcttcgct
     4201 cagtggcacc tgatgcggag gcggtgcgag cgttacaccc acacaattgc gcacctgccc
     4261 gcgcaggctt cgtgcaccat gatgcggata gtatccggtc tcccggacaa tttgccacac
     4321 cttctggcgc gtgctttcct tgacgccggg caggttattg attaccttgc tgacagtctt
     4381 ggtggaaacg ccggacttgt gcgcaatgtc ataaatagta catcgttgct tcatcacaac
     4441 tctttctcct gattatccga gaatccgtgt atttctttct tcacaaagaa caggactgtt
     4501 ccctgccttg tcaaaaaagg caggaagccc ttcaacagaa gactcccggg cttcctgcaa
     4561 caacgcatca tctgaaacgg ccgtgtgcta ctgcagcatt tttccctgat aggcacctgt
     4621 aagtgaatgc agaaatgccg tgatttccgc ggtctggctt gcctgcggtt ttttgccgac
     4681 ctggtatttc accatggttt ccaccgcttc ttcaagcgta ttagcagagc catcatggaa
     4741 gtacgggaac gaaacagcga tattgcgcag cgtgggtgtt ttcagacgga agcggtcaaa
     4801 ttcatcaccg gtgtgattga agcgtccgta atccggtttg acaacgttgc cccgatcccg
     4861 aaagtaatca cggcgcagcc ccatgggctc ataggatttt ccgccgagaa tcactccatt
     4921 gtggcaggta tagcagccta agtcctgaaa atgctgaaag cctgctttct gctcagcgga
     4981 caaggctgaa gcatctcctt tgaggaaagc atcaaaggga gccggagtca gcagtgtctt
     5041 ttcatattcg gcgattgcgt cggtgaaatt tgctttgctg taaccgtctg ggtacacagc
     5101 cagaaattct tctgtgaaaa catcatcctg cgtcagctta ccgataacct gatcccagtt
     5161 cgaagccatc tccacgggat tattcaccgg tccgtccgcc tgatctgcca gatccgtcgc
     5221 acggccatcc cagaactggg cgaactgaaa cacggagttg tagacggtgg gtgaattgat
     5281 atcacccatg gcgccatcga caccgggcga gaatttcttc tgatcggtac cgccgagatc
     5341 aagagcatgg caggaggcac aggaaatggt atcgtcttta gacaggcgta cgtcgtggaa
     5401 cagtttttc
//
LOCUS       DAOPWB010000179         5247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_15781_length_5247_cov_4.516757, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000179 DAOPWB010000000
VERSION     DAOPWB010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15781_length_5247_cov_4.516757"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..428
                     /locus_tag="PKN92_12170"
     tRNA            352..428
                     /locus_tag="PKN92_12170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:386..388,aa:Arg,seq:tct)
     gene            601..2739
                     /locus_tag="PKN92_12175"
     CDS             601..2739
                     /locus_tag="PKN92_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNZ49590.1"
                     /translation="MTTLNSTQQAAVSAASGPVLVLAGAGSGKTRVIIERMAWLVEEE
                     GVSPRSLLALTFTNKAASEMRTRFARRLGVETVPAFIGTFHSFGLWVLRREAETLNLS
                     PNFTIFDDSDQLSLMKRLVKDLPKSFLAVSPRDALRFISQIKQVEPYPNWSDPAESLE
                     EKSFRELWARYHDALKACSALDFDDLTALTAHLFSTSPDACERYRRRFAHVLVDEYQD
                     TNRSQYVIARSLGLDSNLFVVGDEDQSIYSWRGADINNILDFVKDFPRAQAFRLEENY
                     RSTQKILDAANAVVANNVNRLGKTLFTRQQEGEAVRLFSAESAEEEAAFIAEDYLRRQ
                     LPAGGTAVFYRAHALARLLEEALRERHIPYVVVGGIKFYSRREIKDLLAYLRLAANPQ
                     DDESLRRVLNVPVRGIGNTTRERLEEYARLRSQPLLQVLRECEFDETLPARARSAALE
                     FVEIIDFLAHQAKNHPLAALVEKLIDRIGYRDYVRKSDEKEFQNRIEIVDEFVISCRQ
                     YDAGKSGGLEGFLQDLALQADADQWDAASGTVTLMTCHSAKGLEFDHVYLLGMEEDLF
                     PMVRFDDDEEDMEEERRLCYVAMTRARKTLTLTYSQSRMVYGVSHNRRAPSRFLKEAG
                     LHRLKAVDYSGREKTGVKADTPVPTAAAAPAEVELRNGMAVRHARFGRGIVLYTKGAG
                     ERLQARIRFDTGRVAMLLVAKAPLEILAKK"
     gene            2800..3876
                     /gene="pheA"
                     /locus_tag="PKN92_12180"
     CDS             2800..3876
                     /gene="pheA"
                     /locus_tag="PKN92_12180"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010880586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNZ49591.1"
                     /translation="MEQDLEELRAKIDALDSKIIEALNERANAARAIGELKRSAQAPI
                     YVPEREKNVLDRLVARNQGPLNNKAVISIYREIISAIRALEKPTSVAFLGPRDTFSHM
                     AALRVFGLAAEYLPLPSFLDVFTEVERRRIDYGVVPVESSMGGSVSDTLDKFISSNLK
                     VVNEVLLHITQNLLSNSPLSEVRRVYSKDNAILQCRNWLRANLPGAELIETSSTAEAA
                     RRAAEEPGTAAIASRLAAQTYNLEILAERIEDAPHNYTRFVVIGQQMVKRTGDDKTSI
                     LVWVKDKPGALYNMLLPFSRRNINLTRIESRPSQQKAWEYVFFIDFLGHVEDEMVQEV
                     LAEVGDHARNLKVLGSFPRAELQE"
     gene            complement(3960..>5247)
                     /locus_tag="PKN92_12185"
     CDS             complement(3960..>5247)
                     /locus_tag="PKN92_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNZ49592.1"
                     /translation="YNATARTIMSLFLMVVYVCVTIVAVVYSGGLTIHAIFGWPLVPS
                     IWLIGIFAALYTSFGGLKAVIWADLIQGSALILGGAIVLYFGLHAMAPDGGLLAGWSA
                     FMEQAGDKMHMSLAMDHPELPFSALIFGIWIPNFYYWGLNQYITQRTLASKSLKEGQK
                     GVLFAAALKLLIPFVIVIPGIMAWLLFADQLGDGTTAKDAAYPVLIRELIPAGLTGFM
                     LAALAGAVISSLASMLNSASTIFTMDLFKRYLKQDATESQMVLVGRFTTLLFVVIGCL
                     IAPMLANPRFGGVFKYIQDFQGFISPGILTAFVFGLVIKRTPAAAAIAAMVANVPIYG
                     LLHFSSVQTALVKKLPFIGFMDENGVGNIAFLNQMAITAVCLIIVMAIITAIKPLKAP
                     VTMPVNKDFDLSTSPLIPVLGALVILITAWFYYIFW"
BASE COUNT         1231 a         1296 c         1528 g         1192 t
ORIGIN      
        1 cggggggtgg gcgtaacggc ggaagggaga aatggcaggg gcgtgtgtag gactgctgga
       61 ggaggtatgt ctcagggcat gtttccgaag cgaatttgag ggacagtctt cgtcatgacc
      121 cgcttgtgtg ccaatgcgga aggtggccca agcaaaaaga gccgtttcct gttcaagtca
      181 ggagacgtaa aaggttgatg tccatgtgtt catatgctat cattatataa gaactctgct
      241 gccggaacgc cgagaaacac cgcttttttt caggggatat gttacttaag tggcatagaa
      301 tgttttgtcg gttccgcaag cggttcggca acgaaaaata tgtttttgtg tgcgcctgta
      361 gctcagttgg atagagcatc tgccttctaa gccgagggtc gcaggttcga atcctgccag
      421 gcgcgccatt tttattttat gcctgtaaat ccgccttttc tactgtatcc cgggaaggaa
      481 agggaggagc cgctgtacgg gacgtgcgtt ttgtcccgac ggcatactga caccccacgc
      541 aacggcggcc gccggatgca ccatccttct tttatggcac aacgggatct ttgacctatt
      601 atgaccacac tcaattcaac acaacaagcg gctgtcagcg ccgcttccgg tcctgttctg
      661 gtgcttgccg gagcgggcag tggaaaaacc cgagtcatta ttgaacggat ggcgtggctt
      721 gtggaagaag agggtgtgtc tcctcgcagc ctgctggcac tcacattcac caacaaggcg
      781 gcttcggaaa tgcgtacccg ctttgcccgt cgcctgggtg tggaaacggt tccggctttt
      841 atcggcactt ttcactcctt cggactgtgg gtactgcgcc gcgaggcaga gacgctgaat
      901 ctttcgccca attttactat ctttgatgat tcggatcagt tgtctctgat gaagcgactt
      961 gtaaaagatc ttcccaaaag ttttctggct gtatcgcccc gtgatgcgct gcgttttatc
     1021 agccagatta agcaggtgga gccttatccc aattggagcg atccggcgga atctcttgag
     1081 gaaaagagtt ttcgtgagct ctgggcgcgg tatcatgatg ccctcaaagc gtgctccgct
     1141 ctggattttg atgatctgac tgctctgaca gcgcaccttt tcagtacttc gcctgacgcc
     1201 tgcgaacggt acaggcgtcg ttttgcccat gttctcgtgg atgaatatca ggataccaat
     1261 cgcagccagt atgtgattgc gcgcagcctt ggtctggatt ccaatctttt tgtggttggt
     1321 gatgaggatc agagtattta ctcgtggcgc ggcgctgaca tcaacaatat tctggacttt
     1381 gtcaaggact ttccccgggc gcaggccttt cgtctggaag aaaactaccg gagcacacag
     1441 aagatactgg atgctgccaa tgcggtggtt gccaacaatg tgaatcgcct gggcaaaacc
     1501 ttgtttaccc gccagcagga aggggaagcg gttcgtcttt tttccgctga atcggcggaa
     1561 gaggaagccg cttttattgc tgaggattat ctgagacggc agctgccggc gggcggcacc
     1621 gctgtttttt acagagccca tgcactggca cggcttctgg aagaggcact cagggagcgc
     1681 cacatccctt atgttgttgt gggcggcatt aagttttaca gtcgtcgcga aatcaaggat
     1741 ctccttgcgt atctgcgtct tgccgccaat ccgcaggatg acgaatctct gcgccgggtg
     1801 ctcaatgttc ctgtgcgcgg catcggcaac acgaccagag agcgtctgga agaatacgcc
     1861 cggctgcgca gccagccgct gcttcaggta ctgcgtgagt gcgagtttga cgaaactcta
     1921 cctgcccgtg cccgcagcgc cgctctggag tttgtggaaa tcattgattt tctggctcat
     1981 caggcaaaaa atcatccgct ggcagccctg gttgaaaaac tgatcgatcg tatcggctac
     2041 cgggactatg tgcgcaaaag tgacgagaag gaatttcaga acaggataga gattgtcgac
     2101 gagtttgtta tttcatgcag acagtatgat gcaggcaaat cagggggtct tgagggtttc
     2161 ctgcaagatc tcgcactgca ggcggatgcc gatcagtggg atgccgcttc cggtaccgtg
     2221 actctgatga cctgtcattc cgccaagggg ctcgagttcg atcacgttta tctgctgggc
     2281 atggaagagg acctctttcc catggttcgc tttgacgatg acgaagagga catggaggag
     2341 gaacgccggc tctgctatgt tgccatgacg cgggcacgga agacacttac cctgacttac
     2401 tcacagagcc gcatggtcta tggtgtgtcg cacaaccgcc gggcaccaag ccgcttcctg
     2461 aaagaggccg gtctgcaccg gcttaaggcg gtcgattatt ccggcaggga aaaaacaggc
     2521 gtaaaggcgg acacgccggt tccgactgcc gccgcggcgc cagctgaagt ggaactgcgc
     2581 aacggcatgg ctgttcgaca tgcacgtttc ggccgcggta ttgtattata tacaaaaggt
     2641 gctggtgagc gattgcaggc acgcatccgt tttgacacag ggcgtgttgc catgcttctg
     2701 gtggcaaaag cgccgctgga aatacttgcg aaaaaataat tgcccttctt caatgactga
     2761 atgggcggcg tcgtaaagct gaagctcaaa ggattgttca tggaacagga tctcgaagaa
     2821 ctgcgcgcaa aaattgatgc gcttgacagc aaaatcattg aggcgctgaa cgaacgtgcg
     2881 aatgccgccc gtgccattgg tgaactgaag cggagcgctc aggcgccgat ctatgtgccg
     2941 gaacgcgaaa aaaatgtact ggaccggctg gttgcacgca atcagggtcc cctgaacaat
     3001 aaagcggtca tttccattta tcgcgaaatc atcagtgcga tccgtgcact ggaaaagccg
     3061 accagcgttg catttctcgg accccgggac accttcagtc atatggcggc tcttcgtgtt
     3121 ttcgggcttg ccgccgaata cctgccgctt ccgtcttttc tggatgtttt cactgaagtg
     3181 gagcgccggc gcatcgatta tggtgtggtg ccggtggaaa gttccatggg cgggtctgtg
     3241 agcgacacgc tggacaagtt tatctcttcc aatctgaagg tggtgaatga agttttgctg
     3301 cacattacgc agaacctgct gtccaattcc ccgctcagtg aagtgcgtcg tgtctattcc
     3361 aaggacaacg ctattctgca atgccggaac tggctgcgcg ccaacctgcc gggagcggaa
     3421 ctgatcgaga caagcagcac ggcggaagcg gcacgacggg cagcggagga gccgggcaca
     3481 gccgccattg ccagccgtct tgccgcacag acttataatc tggaaattct ggcggaacgc
     3541 attgaagatg cgccgcacaa ctatacacgt tttgttgtca tcggacagca gatggtaaaa
     3601 agaaccggtg atgacaagac atcgattctt gtctgggtca aggacaaacc cggagcgctg
     3661 tacaatatgc tgctgccctt ttcccggcgc aatatcaatc tgacccgcat tgaatccaga
     3721 ccctcccagc agaaagcgtg ggagtatgta ttcttcattg attttctcgg gcatgtggaa
     3781 gatgagatgg tgcaggaagt gctagcggag gtgggggatc atgcacgtaa tttaaaagtg
     3841 ctgggctctt ttccacgggc ggaactgcag gaatagccac tgcttcagtc tgatataaaa
     3901 gaagagcact gcctggtttc gggcagtgct cttttgctga tacagacgac cggttctgtt
     3961 taccagaaga tgtaatagaa ccacgccgta ataagaataa ccagtgcgcc cagcaccggg
     4021 ataagcggtg aggtgctcag atcgaaatcc ttgtttacag gcatggtcac cggtgctttc
     4081 agaggcttga ttgccgtaat gattgccatc acaataatca ggcaaaccgc cgtgatcgcc
     4141 atctgattca ggaaagcaat attgccgaca ccgttttcat ccataaagcc gatgaaggga
     4201 agttttttta ccagagctgt ctggacagag gagaagtgga ggagaccgta aataggcacg
     4261 ttggcgacca tggcggcaat ggcggcggct gccggtgtgc gtttaatcac cagtccgaag
     4321 acaaaagcgg tcaggatacc cggggagata aagccctgaa agtcctggat gtatttgaag
     4381 acgccgccga agcggggatt ggcaagcatg ggcgcaataa ggcagccgat aactacgaaa
     4441 agaagggtag tgaacctgcc tacaagcacc atctggcttt cagtggcatc ctgcttcagg
     4501 tagcgtttga aaagatccat ggtgaaaatg gtggaagcgg aattcagcat ggatgccaga
     4561 gagctgatga cagcacctgc cagtgcggcg agcatgaagc cggtgagtcc cgccggaatc
     4621 agttcgcgga tcaggacagg ataggcggca tcttttgcgg tggtgccgtc gcccagctga
     4681 tcggcaaaaa gcagccaagc catgatgccg ggaatcacaa taacgaaggg gataagcagt
     4741 ttgagggcgg cggcaaagag gacacccttc tgcccttctt tcaggctttt ggatgccaga
     4801 gtacgctgag taatgtactg gttcagtccc caatagtaaa agttgggaat ccagattccg
     4861 aagataaggg cggaaaaggg aagttccggg tgatccatgg cgaggctcat gtgcattttg
     4921 tcgcctgcct gttccatgaa cgcactccag cctgcaagca gtccgccgtc cggtgccatg
     4981 gcatgtagcc cgaaatagag aacaatggcg ccgcccagga tcagtgcgga tccctgaatc
     5041 agatccgccc agatgaccgc tttcagcccg ccgaaggaag tgtagagggc ggcaaaaata
     5101 ccgatcagcc atatgctcgg caccagcggc cagccaaaaa tggcatggat ggtcagaccg
     5161 ccggaataga cgacggcaac gatggtcacg cagacataca caaccatgag gaagagggac
     5221 atgatggtgc gtgcagtggc gttgtac
//
LOCUS       DAOPWB010000180         5236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_15821_length_5236_cov_4.417680, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000180 DAOPWB010000000
VERSION     DAOPWB010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15821_length_5236_cov_4.417680"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PKN92_12190"
     CDS             complement(<1..979)
                     /locus_tag="PKN92_12190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNZ49593.1"
                     /translation="MSERILWLNSSTASHEKTIQCLHAASFELDIAKSHADALMRLQE
                     ASYQVFIAQQHCNGQDCLPLLREVSVRRPETVCILLGAAATEADNCPGSFVFLSGAGE
                     PEQLLADVQHALKQAGLTRCTTLAEEVEKTRNELLRTRERLNNLLDSTMDAVFTLGTT
                     LEITYANRGAVRMLGYNFNEFEGMDFELLLRGAEDESSLLLTQLDDGPIQNYETELRH
                     QGGRWVPVSISLSKAYDHLGKFVSLLAICKNITQQKQLEQKLKEKIVTDSLTGLYNQR
                     YFYERLAAEIERARRQNHPLSLLLLDVDAFKQYNDSRGHLEGDRVLKAIG"
     gene            complement(1137..1367)
                     /locus_tag="PKN92_12195"
     CDS             complement(1137..1367)
                     /locus_tag="PKN92_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49594.1"
                     /translation="MKNSSFLKLVLLCCLLTAVLWVSTMDEAQAQTADQKLAQQSGLG
                     SKKIDADKLPGKLEYTIAIGSVVAMIAVVKFA"
     gene            1481..2593
                     /locus_tag="PKN92_12200"
     CDS             1481..2593
                     /locus_tag="PKN92_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49595.1"
                     /translation="MRSILIGITARGCAGRIDIFPGAGTKATESRAGLTVYPVFAVRY
                     CHSKAEKPAESDAVIHVFSMNVLQDSTQPFFYVTGGVILALFLGVGIWSLHVRFHLQQ
                     EWSVRRQLVVLGMVLLFFGYEIITVQQLIQDDIALFIFTLLGLLVAGMALYGHLVVSL
                     LSRILVELILPDHPGISTAPQLEHAAQLEKQGDWEGALKEYFVLVHIYPGNPAVLSRI
                     TANLICQKRYTEASHWFDRLVTYAERPDEARALLEQMAAAFTDEKDLPLLRNGMFCFL
                     QRFPQQTEIKAQLAHLDAEDAGRPRPLGTGISGEKPGSSSFQSSAAVGSALLPMRDLS
                     ESTAEVLRQPEAKELSAGVGALLQPLQDHPIGDPKR"
     gene            2690..3757
                     /gene="corA"
                     /locus_tag="PKN92_12205"
     CDS             2690..3757
                     /gene="corA"
                     /locus_tag="PKN92_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNZ49596.1"
                     /translation="MSHQLTCPYGSKGSQPPGSLVHVGTAHTAVPEITLICFGPEHHA
                     IRKGISLEEAAKPDTRAEVVWLDITGLHDVSLLKEAGERFSIFPLTLEDILNTHQRPK
                     AEFFEQYVFCVLKVAEPHPSGRGLVMHQLSLILQPGRVISFQEKPNKILHPLIHRIET
                     AAGRIREFGADYLFHAILDIVVDQYFLCLELLGEEIEGLESRALDRPDQALVPELQKM
                     KRELLFLRKALWPLREAVNALLRDGHPLIKRDTDAYFRDLYDHTVQIIDTVELFRDMA
                     SGLLDVYLSSINNRMSEVMKILAIISTIFMPMSFIAGIYGMNFRHMPELDVPWAYPLV
                     LLLCLSIAGGMLVFFRRRHWL"
     gene            3806..5194
                     /gene="hslU"
                     /locus_tag="PKN92_12210"
     CDS             3806..5194
                     /gene="hslU"
                     /locus_tag="PKN92_12210"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNZ49597.1"
                     /translation="MQSLTPQEIVQELDKYIVGQTQAKRKVAIALRNRWRRLQLPEAL
                     QEEVVPKNIIMIGTTGVGKTEIARRLSKLAQAPFVKVEASKFTEVGYVGRDCESMIRE
                     LTEVAVKMAREELRGECEARAKEKAEQRLLDLLLPPTMRHAERGGYNPADPDAGDEPP
                     VERGAAAQEKARNLIRTKLAAGDLEDKEVELTVTESKDNAMLQIFASSGMEEMGMNLQ
                     DVFGHMTPKRRKAKKVTVAEAREVLLQEAFNELIDMDLAARRGVENAENSGIVFLDEI
                     DKIAGRGHRGHGPDVSREGVQRDILPIVEGSTVLTKYGSVRTDHILFIAAGAFHMTQV
                     SDLIPELQGRFPIRVELDSLSRDDFLRILTETEYSLIKQYQAMLEAEEVTLHFEPEAL
                     VKIAEICERVNSETEDIGARRLHTILEYVLEDISFDASNRKGTQVSITAEFVESRISE
                     IAEKRDLSQYVL"
BASE COUNT         1195 a         1319 c         1462 g         1260 t
ORIGIN      
        1 cgccgattgc cttgagaacc cggtcccctt caagatgccc gcgactgtca ttatactgtt
       61 tgaaggcgtc cacgtccaga agaagcaggg acagggggtg attctgccgc cgtgcacgtt
      121 ctatttccgc cgccagacgc tcataaaaat aacgctggtt atacagcccg gtgaggctgt
      181 cggtgacaat tttttccttc agcttctgtt ccagctgttt ctgctgtgta atgtttttgc
      241 agatcgcaag aagggatacg aacttcccca gatgatcata cgctttggac agggaaatac
      301 tgacgggtac ccagcggccg ccctgatggc ggagctctgt ctcgtaattt tgtatagggc
      361 cgtcgtccag ctgcgtcagg agcagtgaag actcatcttc ggcgccccgg agcagtaatt
      421 caaagtccat cccttcaaat tcattaaagt tatagccgag catgcgtact gcaccccggt
      481 tggcataagt gatttccagc gtggtgccca gtgtgaaaac agcatccatg gtgctgtcca
      541 gcagattatt cagccgctcc ctggtgcgca gcagctcgtt tcgtgttttt tcaacttctt
      601 ctgccagcgt ggtacatcgg gtcagtcccg cctgcttcag ggcgtgctgt acatcggcga
      661 ggagctgttc gggctctccg gcaccggata gaaagacaaa ggagcccggg cagttgtccg
      721 cttcagtggc tgcagctccc aacagtatgc atactgtttc tgggcgccgt acactgactt
      781 cccgaaggag cggaaggcaa tcctgaccgt tacagtgctg ctgggcgata aagacctggt
      841 aggaggcttc ctgcaaacgc atcagcgcgt cagcatggct tttggctatg tccagctcaa
      901 atgatgctgc atgcaggcac tgtattgtct tttcatggga tgctgtgctg ctattcagcc
      961 acaaaatacg ttcggacaca ggaattcctc gtttcattcc ggggcggccc tgcgacccgg
     1021 ctgctcttat tgatcaacag aactcccaaa ctgaaagtga tccggcatcg atgaccggag
     1081 tttccgatct gcctgcgcaa ccgaattgcg atacactttg cggctatccc aacccttcag
     1141 gcaaacttca cgacggctat catggcgact acggagccga tggcaatggt atattccaat
     1201 ttccccggaa gtttgtctgc atcgattttt ttggaaccca gtccgctctg ctgcgccagt
     1261 ttctgatcgg ctgtttgcgc ttgcgcctca tccatggtgc tgacccacag gacagccgtc
     1321 agcaggcagc acagtaatac taacttaaga aaagaactat ttttcatggt tgctgctcct
     1381 tagttatctc ggtttcagac ttcatctctg taataataat accgttattc tcttttttat
     1441 gcaagtaaag caccccgttg cgacagcaaa ttcacgggat atgcgctcaa ttttgatcgg
     1501 tatcacagcc cggggatgtg ccgggcggat cgatattttt ccgggtgcag gcacaaaagc
     1561 gacggaaagc cgtgccgggt tgactgttta ccccgtgttt gcagtacgct actgtcatag
     1621 caaggcggag aagcccgcag aaagtgatgc agtgatccat gttttttcca tgaatgtgct
     1681 gcaggacagc acccaacctt tcttttatgt caccggaggg gtgattctgg ctctttttct
     1741 cggagtcggc atctggtccc tccatgtccg ctttcacctg cagcaggaat ggagtgtgcg
     1801 ccggcagctt gttgttctcg ggatggtgct tctctttttc ggttacgaaa tcattacggt
     1861 gcagcagttg attcaggatg atatagcctt gtttatcttt acgctgctgg ggcttctggt
     1921 ggcgggcatg gcgctttacg gtcatctggt ggtgtcgctg ctgtcccgta ttctggtgga
     1981 actgatcctt ccggatcatc ccggcatttc cactgcgccc cagctggagc acgctgcaca
     2041 gcttgaaaaa cagggggact gggaaggcgc tctgaaagag tattttgttc ttgtccacat
     2101 ttatccgggc aatcctgccg tcctttcccg gattaccgcc aatctgatct gccagaaacg
     2161 atatacggaa gcaagccact ggtttgaccg tctggtcacg tatgcggaac gccccgacga
     2221 agcccgggcg cttctggagc agatggcggc tgcttttaca gacgaaaagg atctgcccct
     2281 gttgagaaac gggatgtttt gctttttgca gcgttttccc cagcagactg aaatcaaagc
     2341 gcagcttgcg catttggatg cggaagacgc aggaaggccc cgcccgctcg gaaccgggat
     2401 ctccggggaa aagccgggca gctcgtcgtt ccaaagcagc gccgcagtcg gttccgccct
     2461 cctgccgatg cgggatcttt cggaatcgac ggcggaagtc cttcggcagc cggaagcgaa
     2521 agagctcagt gcaggcgtcg gtgcgctgct gcagccgctt caggatcatc cgataggcga
     2581 tcctaaacgg tgatctgacg gtgctgccgg atgccttcgc tgcaccggtg cccgcataaa
     2641 ggtggtatac tacttgaaac aggtctccac tggagtaaag ggaaatggtg tgtcacatca
     2701 attaacctgt ccttacggaa gtaaaggatc gcagccgccc ggttcactgg tgcatgtcgg
     2761 caccgctcat acagcggtgc ctgaaattac gctgatttgt ttcgggcctg aacaccatgc
     2821 catccgcaag ggcatatctc tcgaggaagc agcaaagccg gatacccggg cggaagtggt
     2881 ttggctggat ataaccggtc tgcatgatgt ttcactgctg aaggaagcgg gcgagcgttt
     2941 ttcaatcttt ccgctgacac tggaggatat tctgaatacc caccagcggc ccaaggcgga
     3001 gtttttcgag cagtatgttt tttgtgtgct gaaagtggcg gagccgcatc cgtcgggaag
     3061 agggcttgtg atgcatcagc ttagcctgat attgcagccc ggacgggtga tcagttttca
     3121 ggagaagccc aataaaatac tgcatccgct gatccatcgc atagaaacgg cagcaggccg
     3181 tattcgtgag tttggtgcgg attatctttt tcatgcgata ctggatattg ttgtggatca
     3241 gtattttctg tgtctggaac tgttgggtga agaaatagaa gggctggaaa gccgcgccct
     3301 ggatcggccc gatcaggcac tggtgcctga actgcagaaa atgaaacggg agcttctttt
     3361 ccttcgaaaa gcgctttggc cccttcgtga ggcagtcaac gcgctgcttc gtgacgggca
     3421 tccgctgatt aaacgtgata cagatgccta tttccgtgat ttgtacgacc ataccgtcca
     3481 aatcatcgat acggtggaat tattcagaga catggcatcg gggcttctgg atgtctatct
     3541 cagcagcatc aacaatcgca tgagcgaagt gatgaaaatc ctcgccatca tctcgacaat
     3601 ttttatgccc atgtcgttta tagccggaat ttacggtatg aatttccggc atatgccgga
     3661 attggatgta ccctgggcat acccgctggt gctcctgctt tgcctgagca tagcaggcgg
     3721 aatgctggtt tttttccggc gcaggcactg gttataatac ccgcttacat catctttcca
     3781 aaatgcccgt gaagaggaca attcgatgca gtcattaacc cctcaggaaa ttgttcagga
     3841 actggacaaa tatattgtcg gtcaaaccca ggcgaaacga aaagttgcga ttgcgctgcg
     3901 aaaccgctgg cgccgccttc agctgcctga agcacttcag gaagaagtag ttcctaaaaa
     3961 tatcattatg ataggaacca ccggtgtcgg taaaacggaa attgcacgcc gcctgtccaa
     4021 acttgcacaa gcgccctttg ttaaagtgga agcgagcaaa tttacagaag tgggttatgt
     4081 gggacgggat tgcgagtcca tgatccggga acttacggaa gtggcggtga aaatggcgcg
     4141 ggaggagctg cgtggggaat gtgaagcccg ggcaaaggaa aaggcggagc agcgtcttct
     4201 ggatcttctg ctgcccccga ccatgcggca tgctgaaaga ggaggataca atcctgccga
     4261 cccggatgcc ggagatgagc cgcctgttga acggggggct gccgctcagg agaaagcaag
     4321 aaacctgatc cgaaccaaac tggcggcagg ggatctggaa gacaaggaag tggaactgac
     4381 cgttaccgag tccaaggaca acgccatgct ccagatattt gcttccagcg gcatggaaga
     4441 aatgggcatg aatctgcagg atgtcttcgg gcacatgacg ccgaaacggc ggaaagcgaa
     4501 aaaggtcacc gtggcggaag cgcgggaagt gcttctgcag gaagcgttca atgagctgat
     4561 tgacatggac cttgccgcgc ggcgcggtgt tgagaatgca gaaaacagcg gtattgtttt
     4621 tctggatgaa atagacaaga tagcaggacg tgggcatcgg ggacatggtc ccgacgtgtc
     4681 ccgcgagggt gtccagcgcg atatcctccc catcgtggaa ggcagcacgg tactgacgaa
     4741 atacggttca gtccgaaccg atcacattct tttcattgca gccggcgctt ttcacatgac
     4801 ccaggtgtcc gatctgatcc cggaacttca gggacgcttt cccatacgtg tggagctcga
     4861 cagcctgagc cgggatgatt ttttgcgtat tctcaccgag acggagtatt cgctgatcaa
     4921 acaatatcag gccatgcttg aagcggaaga agtcaccctg cattttgaac cggaggctct
     4981 ggtaaaaatt gcggaaatct gtgagcgcgt gaacagtgaa accgaagata tcggcgcaag
     5041 gcggcttcat acgattttag agtatgtgct ggaagacatc tcttttgatg catccaaccg
     5101 caaagggacg caggtttcga tcacggcgga gttcgtggaa agcaggattt ccgaaatcgc
     5161 ggaaaaacgc gatttgagtc aatatgtgct ttgaggcgaa aatgcgccat gatacacttt
     5221 tcgctcatgc aggagg
//
LOCUS       DAOPWB010000181         5201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_15979_length_5201_cov_4.179557, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000181 DAOPWB010000000
VERSION     DAOPWB010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15979_length_5201_cov_4.179557"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /gene="cgtA"
                     /locus_tag="PKN92_12215"
     CDS             <1..937
                     /gene="cgtA"
                     /locus_tag="PKN92_12215"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HNZ49598.1"
                     /translation="GQRWVAARGARGGRGNARFVTSTNRIPRFAEKGEPGEEKEYILE
                     LKLIAEAGLVGLPNAGKSTFLTVVSAARPKIGDYPFTTLSPNLGVALLSDHRMLTIAD
                     IPGIIEGAAEGKGLGHDFLRHIERTLALVFLIDLGDADPLDTVNILESELIQYNPELG
                     ERPRLYAFNKSDVPENSETFRKLQETLPPDSFLISAATGEGVPPLLNRLWHVVEQARE
                     AEREEAEREEREAPERAYMFEAPFEVERIEAGFQISGEKILRIVAMTDFDNPEAIAHF
                     QRRLDKLGVFKALKRMKAQPGDTVVIGDFEMEYEE"
     gene            1038..1820
                     /locus_tag="PKN92_12220"
     CDS             1038..1820
                     /locus_tag="PKN92_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49599.1"
                     /translation="MSSKKRYQPGDPRLIVLWARRYAQSRTISFLVQWVFIVIMVLVI
                     GLAAGLTQIAHEQHRMGLFTLSIGAMVLSALIMLWFSMSRWSSDLIFSITNWLYGREG
                     YASWTGSSGKEGSLPLWTTGLSGGLLAYHLLGALLISFNYMPIGLLQPWSALYMTPYL
                     IIMVLYQQLGFWAWIWPILYGIHGVLLYFGVPLRFGREYELLNIVVPIFGYGLIAILT
                     GHIYSRYALLKLKTLTRNAIPDREETQDEEAEEVQAGDQGHG"
     gene            1813..2118
                     /locus_tag="PKN92_12225"
     CDS             1813..2118
                     /locus_tag="PKN92_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNZ49600.1"
                     /translation="MDDLDRTIHEPARLRILTILSGVDAADFNFLLNTASLTKGNLSS
                     HMDRLEKAGYVSVEKSFKGRMPHTKFSITEEGASALKKYWEGLDAIRAMQDQGDNSA"
     gene            2232..3158
                     /locus_tag="PKN92_12230"
     CDS             2232..3158
                     /locus_tag="PKN92_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNZ49601.1"
                     /translation="MQTAHLPVPFLFHVCLTLLIIGVASLGAEAATLTEIYPEMTSSL
                     LADALLEKLPEGTLLQAGSVIIQQTDLDQMKSDMKEKIKEDTFVSELGILQNLALDKI
                     LEAEVLRDKNKSDYDENELKVLFQEFAQSKVAHAIVTDEEADAFYAANQELFGGRSLA
                     DLKPAIKQTLQREKSQAAWIDYLKSLGSSIPIRLQADWVKADVERFSSSPVETARKTG
                     KPVFVDFSASWCGPCKRLKPIVESLQKKYADKMVFVILDIDEQPFLAQHYGASSVPHL
                     FFYDAEGRAAGDILGFVPEETLVKKFAELGVQ"
     gene            3383..4048
                     /locus_tag="PKN92_12235"
     CDS             3383..4048
                     /locus_tag="PKN92_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNZ49602.1"
                     /translation="MENSVLLKKSVALVAFSRQSLFGAPMIETGDNRSALDAGLIARA
                     QAGDISAFEDLVRRYRNEVYALCFYFTRNREDAWDLSQEVFIKAWRALSRFRGDSSFK
                     TWLMRIAANHSKDHLKKRRIQLTEYDDALQVPDNRDNRTPRSEAEAKEIGTAIDAAVR
                     QLPPKLQMAFILREYQGLSYQEMAEVMACNMGTVMSRLFNARRRLQQILAPLGLLEGY
                     SND"
     gene            4041..4478
                     /locus_tag="PKN92_12240"
     CDS             4041..4478
                     /locus_tag="PKN92_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49603.1"
                     /translation="MIKRWKFQKIAARIDGELPLSSMPSDPESQRYAHFVETLHKHAQ
                     TLPEAPVIADESMTAFLSGIHEGLDAPVLSTGRLWTMGSALTAALILVLSLLVIFSRG
                     DEKVRATEVESCTTELDGVTIKTFDNRDGVSTVWVTMPENDLW"
     gene            4472..4921
                     /locus_tag="PKN92_12245"
     CDS             4472..4921
                     /locus_tag="PKN92_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49604.1"
                     /translation="MVSSVVLCVLLSSLCAGKDAPDLSLRLREVEAVKEDSSEKTYDR
                     DCSTIKDALDDLPFNRFSMLSDKKLTLTADKESRVPLAAAYVLSLNYEGVDESGRARL
                     QVGVELAGSAPAKKVVETVLLLAPNKKARICGLRQDKGELVLVFSTP"
     gene            complement(4947..>5201)
                     /locus_tag="PKN92_12250"
     CDS             complement(4947..>5201)
                     /locus_tag="PKN92_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNZ49605.1"
                     /translation="GAPSLFDFQWPRPVAIVFGNEENGINARVLARCDGAVQIPMAGY
                     KKSINVATAFGIVLYHILSRWGMPETSLPEEERERMLRVE"
BASE COUNT         1271 a         1311 c         1351 g         1268 t
ORIGIN      
        1 cgggcaaaga tgggtggcgg ccaggggcgc caggggcggc agggggaatg cgcgctttgt
       61 cacttccacc aatcgtatac cccgttttgc ggaaaaagga gagcccggcg aggaaaaaga
      121 atacatcctt gaattgaaac tgattgccga ggcaggtctg gtcgggcttc ccaatgccgg
      181 caagtccact tttctgacag tggtaagtgc ggcacgtcca aaaattgggg attacccctt
      241 taccaccctt tctcccaacc tcggtgtagc gcttctctcc gatcaccgaa tgctgactat
      301 tgcggatatt ccgggcataa tcgaaggggc agcggaaggc aaagggctag gccatgattt
      361 tctccggcac attgagcgca ctctggcact cgtgttcctt attgatctgg gcgacgccga
      421 tccattggac actgtgaata ttctggaatc ggaactgata cagtataatc cggagttggg
      481 agagcgcccc cgcctttatg cattcaataa atctgatgtt cctgaaaaca gcgaaacttt
      541 tcgcaaactg caggaaaccc tgccgccgga ctcttttctg atctccgccg ccacgggaga
      601 gggtgtgccg cccctgctca accgactgtg gcatgtagtg gagcaagcca gggaagccga
      661 gcgtgaagaa gcagaaagag aagagcggga ggcacctgag cgcgcgtata tgtttgaggc
      721 acccttcgaa gtggaacgca tcgaggccgg tttccagatt agcggagaaa aaatactgcg
      781 catcgttgcc atgaccgatt ttgacaatcc ggaggccatc gcccattttc agcgacgcct
      841 ggataaactg ggtgttttta aagcacttaa acgaatgaaa gcacagccgg gcgatacagt
      901 tgttatcggt gactttgaaa tggagtatga ggaatgagca cagtatagct gtagagttcc
      961 gctgcacacg gcactttgct tttacccggc agttatgata caaccggaac acagcacctc
     1021 cggagaggaa gcaaatcatg tctagtaaaa agcgttatca gcccggcgat ccgcgtctga
     1081 ttgttctgtg ggcacgccgc tatgcccaaa gccgaaccat ttcttttctg gtgcaatggg
     1141 tctttattgt gatcatggtt cttgtaatcg gccttgccgc cggtctcaca cagatagccc
     1201 atgaacagca ccggatggga ctgttcaccc tctccatcgg agccatggtt ctgtccgccc
     1261 tgattatgct ctggttttcc atgtcccgct ggagcagcga tcttattttc agcatcacca
     1321 actggctgta cggacgggaa ggatacgctt cctggacagg cagcagcgga aaagaggggt
     1381 cgcttcctct ctggacaacc ggactgagcg gcggtctgct tgcctaccat ctgctgggag
     1441 ccctccttat cagtttcaat tatatgccta tcggtttgct ccagccctgg tctgcattgt
     1501 atatgactcc ttatctcatc atcatggtgc tgtatcagca gttaggcttc tgggcatgga
     1561 tctggccgat actttacggg atacacggtg tgctcctcta tttcggtgtt ccccttcgtt
     1621 tcggaaggga atatgaactg ctgaatattg ttgttcctat tttcggatac ggacttatcg
     1681 ctattctcac cgggcatatt tacagtcgct atgcgctttt aaagttgaaa acactgaccc
     1741 gtaatgccat tcccgaccgg gaggaaacac aggacgaaga ggcagaagaa gtgcaggcgg
     1801 gagaccaggg gcatggatga tctggatcgt accattcatg aacctgcccg gctccgtatt
     1861 cttaccattc tgtccggagt ggatgcggcg gattttaact ttctgctgaa tacggccagt
     1921 ctgaccaaag gaaacctgtc ttctcatatg gatcgtctgg aaaaagcagg ctacgtttct
     1981 gtggaaaaaa gcttcaaagg acgcatgcct cacaccaaat tctccattac agaagaaggt
     2041 gcttcggcat tgaaaaaata ttgggaagga ctggatgcca tccgtgccat gcaggatcag
     2101 ggagacaatt cagcatgatc ctccgtgcag aagcacagcc tgttttcctg tccggccgcc
     2161 tattcgttag ttgccgcgca agttcaaacc cggggcatta tgcaaagccc cgaaagaaag
     2221 agaagaccct tatgcagact gctcatttgc ctgttccttt ccttttccat gtatgcctga
     2281 cgcttctgat tatcggcgtt gcgagcctcg gggcagaagc agccaccctc acggaaatct
     2341 atccggaaat gacttccagc ctccttgccg acgcacttct ggaaaaactg ccggaaggca
     2401 cgcttctgca ggccggatcc gtcattattc agcagacgga tctggatcag atgaaatcag
     2461 acatgaaaga aaaaatcaaa gaggatactt ttgtgtctga actgggcatc cttcagaatc
     2521 ttgctctgga caaaattctt gaagctgaag tgctgcggga caagaacaaa tccgattatg
     2581 acgaaaatga actgaaagtc ctttttcagg aatttgcgca aagcaaggtt gcccatgcta
     2641 ttgtaacgga tgaagaggca gatgcttttt atgcggcaaa tcaggaactt ttcgggggac
     2701 gctctttagc agacctgaag cccgccatca agcagacgct ccagcgggaa aaatcgcagg
     2761 cagcctggat agattatctg aaatctttgg gctcgtccat tcccattcgt cttcaggcgg
     2821 attgggtcaa agcggacgtt gagcgtttca gcagttcacc tgtcgaaact gcaagaaaga
     2881 ccggcaagcc cgtctttgtc gatttcagcg cctcctggtg cggtccctgc aaacgcctca
     2941 agccaatcgt cgaatcactg cagaaaaaat atgcggataa gatggtgttt gtcattctgg
     3001 acattgacga acagcctttc ctggcacagc attatggtgc ttcctccgtg cctcatctgt
     3061 ttttttatga tgctgaagga cgtgcagccg gggatattct cggattcgta cctgaagaga
     3121 cgcttgtgaa aaaatttgct gaactcggtg ttcagtgacc tgtttctgtt ttcaggtatg
     3181 ctatgcagcc cgtagcaagt gcgaccccga gctgacaaat taattgtttt tattgtttta
     3241 cggctctttt tccctgaaca agcccgacta aggggattta tagagatttt aaaatgaata
     3301 tttccagaag aagcccgtca atattgatag cagggactgt gccctgcaat tcagtaatga
     3361 aaaatctgga gttgctttcc ggatggaaaa cagcgtgctt ctcaaaaaat ctgtcgcact
     3421 tgtcgctttt tcccggcagt ctctgtttgg agcgcctatg atcgaaacag gcgacaacag
     3481 aagcgcactg gatgcgggcc ttatcgcaag agcacaggca ggcgacatca gcgcctttga
     3541 ggatctggtg cggcgttata gaaacgaagt gtatgctttg tgtttttatt ttacccgtaa
     3601 ccgtgaagac gcctgggatc tttcgcagga agtcttcatc aaggcatggc gggcgctgtc
     3661 ccgatttcgc ggcgacagca gttttaaaac ctggctgatg cgcattgccg ccaatcacag
     3721 caaagaccat ttaaaaaaac gacggattca gctcaccgaa tatgacgacg ccctgcaggt
     3781 gcccgacaac cgggacaacc gcacgccccg gagcgaagcg gaagcaaagg aaatcgggac
     3841 agcgattgat gcagctgtcc ggcaattacc gcccaaactc cagatggcat ttatactccg
     3901 cgaatatcag gggctttctt atcaggaaat ggccgaagtg atggcatgca atatgggcac
     3961 cgtcatgagc cgccttttta atgcacggcg ccgcctgcag cagattcttg cccctctggg
     4021 cttattggag ggttattcaa atgattaagc gatggaaatt tcagaaaatt gcggcccgaa
     4081 tagatggaga actgcctctt tcttccatgc cgagcgatcc ggaaagtcaa cgctatgcgc
     4141 attttgttga aacgctgcat aaacacgctc agacacttcc cgaagctccg gtcattgccg
     4201 acgaaagcat gacggctttt ctgtccggca ttcatgaagg gctggatgca cctgttctgt
     4261 caaccggccg cctgtggaca atgggttccg ccctcaccgc agcattaatt ctggttctct
     4321 cccttcttgt tattttttcc aggggcgacg agaaagtccg tgcaacagaa gtagaatcct
     4381 gcacgacgga actggatggt gtcaccatca aaacctttga caacagagac ggtgtttcca
     4441 cagtgtgggt caccatgccg gagaatgact tatggtaagc tccgtggtgc tctgtgttct
     4501 tctctcgtcc ctgtgcgccg gaaaagatgc ccctgattta tcgctgcgcc tgcgtgaagt
     4561 cgaagctgta aaagaagaca gttccgagaa aacctacgac cgggactgtt caaccattaa
     4621 agatgcactg gacgaccttc ccttcaaccg gttttccatg ctgtcggata aaaaactgac
     4681 actgacagcg gataaagaaa gccgtgtgcc tctggctgcc gcctatgtat tgtccttaaa
     4741 ctatgagggg gtcgatgaat cgggtcgtgc cagacttcag gtcggtgttg aacttgcggg
     4801 atctgctccg gcaaaaaaag tggtggaaac tgtcctgctg cttgcgccca ataaaaaggc
     4861 acggatctgc ggattgcgtc aggacaaggg cgaattggtg ctcgtttttt ccacgccgta
     4921 acttttccgg aaggtctgtg cacccgttat tccacacgca gcatccgctc acgttcctcc
     4981 tccggaagag atgtctccgg cataccccat cgtgaaagaa tgtggtagag gactatgccg
     5041 aaagcggtgg cgacattgat gcttttttta tatcccgcca tgggaatttg cacggcacca
     5101 tcgcaacggg caaggacacg ggcattaatg ccattctctt cattgccgaa tacaattgcc
     5161 acaggtctcg gccactgaaa gtcaaaaagt gaaggggccc c
//
LOCUS       DAOPWB010000182         5161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_16157_length_5161_cov_5.503721, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000182 DAOPWB010000000
VERSION     DAOPWB010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16157_length_5161_cov_5.503721"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3260
                     /locus_tag="PKN92_12255"
     CDS             <1..3260
                     /locus_tag="PKN92_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ49606.1"
                     /translation="EVRDRLDLFMPPQELTQKPVILRYDPSLDPVMRVAIVSVDEKHV
                     PGSQEERDALTTIREAAERRLKSDLEAEDGIAQAKVKGGQKEEIQVQLDVENLKSLGL
                     SPEYVITALAQQNINLSGGRLREGEAEYLVRTLNEFMTIEDVANSIILTPGGAHLKLI
                     EIAEVFLGTKERETIVHINGREAVAIDIYKEGDANTVNVCNRVHDLLGFEREYSFQEK
                     LSAAIKEAAMRRQAEKLAASGTVVPAPVAAKPQTGAFLDRLPKYAKPAIISDQSRFIR
                     GAIKEVQQSALIGGLLALLVLYVFLQEMRSTVIIGISIPISVITAFVPMFFQKISLNI
                     MSLGGLALGVGMLVDNSIVVLESVFRCREEGDSLVDAAERGTKEVAGAVTSSTLTTIC
                     VFLPVAFVEGIAGQLFGNLSLTVTYSLLASLLTALYLIPLIVSRQGQELRADRYVIWL
                     VRAYQEGRATVGESKSRAFFSVLPRGIRYAGTYIRTVWRESLEVAFKVFGSALRPPLW
                     KNLPHAVFYVVLLPFLLALFLLRLLSGTTISLLVTGLYLICVFFYGIFRIIRGIFGFV
                     FYIPLKSFALGFEAFRKAYGATLRAFLRFSPAFLLGVVLLSIHAGYLALELGRELMPP
                     MRQGEFGLRVEVQTGTRLEKTEEQMRQFEEILRSTPEVASVTLEVGSERDTASSDKGD
                     NIATFTVLLKEASEMEFNQDEVIERLRENIFAKTTTETVTFTLPTMFSFRNAIETQLF
                     GDVLKDLREYGDKAVALISTVAGVKDAEQSIKPGYPELIIEMDRQALASRGLSPGKVA
                     QRLRTEIQGEVATRFSRGSDKLDIRVRASQKKLSSVEDLRFLSVTDGPAPIPLINVAK
                     LRIEEGPSEIRRVNQKRTVILSANVEGRDLGAVSRDIDSTLEKMALPQNMYFLQGGQS
                     RELDAAFKSLRFVLLLAAFLVYVVMACQFESIIQPALVMFSVPLAFIGVVYTLYLTGT
                     NLNVMVFLGGIVLIGIVVNNAIILVDYTNQLQARGLNITDAIVEAGVARIRPIFMTTI
                     TTVLGLTPMLIASGEGAEMRRPMALTLIAGLSFSTLLTLFIIPMAYYLFGRKKSL"
     gene            3257..>5161
                     /locus_tag="PKN92_12260"
     CDS             3257..>5161
                     /locus_tag="PKN92_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ49607.1"
                     /translation="MKSSLAALALKRPIMVTMLTITMVGLGIISWYRLPLNFLPRAES
                     PFMVCIFPYPGAAPSQVEQQVAIPVEGEFRTLSGLTRLRTNSTDSACYAEMNFSLETD
                     LNQVSAEIRDRIERLKLVLPKEVEKVQLQRFNVDAFPVMAVGLFSHGDREEFTHKVRT
                     IVEPRLRRLDGVASIQVHSPTQPREVLIEFEQDTLTGLNLSMPEIIQYLQIGTMTISL
                     GELTDDAMRYIVRYEGEYRSIEAIADLPIGKQGLHLRDIATVRFSSRSKDLRATMDGA
                     DGLVLFITKESQANTVDTCRSVREELDKLLLTPAFSGARVLPLFDQSEFIVKALNNLF
                     LQGLYGSIMAVGVLFVFMHRFLPTALVALSIPSSLVFALVFMFFTGMSLNIITMVSMI
                     VAVGMLVDNAIVVVENILRHRSLGLSIREAAVKGPQEVSLAIVASTVTTWVVFLPMFY
                     MKTGQMSVFMEQLGEPLIAALGGSLLGALTLIPLLMVGVEFLESRVKRRTAKIQWGSW
                     TKLPGKIVDACVDAYGVALRHSLRQRFVVILLVLLAVLFTALFPMKTVGMRTLPKLDM
                     REVTVDVSLARNFGMDRAVELFKSLEAEIDQLREVLAVKYVLAYHGATGGAIHVYLYT
                     EDDGPIGEDPP"
BASE COUNT         1122 a         1233 c         1416 g         1390 t
ORIGIN      
        1 aggaagtgcg tgatcggctg gatttgttca tgccgccgca ggaactcacc cagaaaccgg
       61 ttattttgcg ctatgatcct tcgctggatc cggtgatgcg tgtcgccata gtctctgtag
      121 acgaaaagca tgttcccggc tcgcaggaag agcgggatgc cctgactaca atccgtgagg
      181 cggcggagcg ccgtctgaaa agcgatctgg aagctgaaga cggaattgcc caggcgaaag
      241 tgaaaggcgg ccagaaagaa gaaatccagg ttcagctgga tgttgaaaat cttaagtcgc
      301 tgggattgtc acctgagtat gtgatcacgg cgctggcgca acagaatatc aatctttccg
      361 ggggccgcct gcgtgaaggg gaagcagaat atctggttcg cacactcaat gaattcatga
      421 cgattgagga tgttgcgaac agcattattc tgaccccggg cggtgcgcat ctcaaactta
      481 ttgaaattgc ggaagtgttt ctgggaacca aagagcgaga gacgatcgtg catatcaatg
      541 gcagggaagc cgtagccatt gatatctata aagaagggga tgccaataca gttaatgtgt
      601 gcaaccgagt gcatgacctt ctcggttttg aaagagaata ctcttttcag gagaaactgt
      661 cggctgcaat caaagaagcg gcgatgcggc ggcaagctga aaagctggcg gcgtccggaa
      721 ctgttgtgcc ggcgcccgtt gctgccaaac ctcagacagg cgcttttctt gacaggctgc
      781 ccaaatatgc caagcctgcc attatcagcg atcagtcccg tttcatccgc ggtgccatta
      841 aggaagtgca gcagtctgcg cttataggcg gtctgctggc actgctggtt ctttatgtgt
      901 ttcttcagga aatgcgttcc acggtgatca tcgggatctc catacccatt tcggttatca
      961 ccgcttttgt ccccatgttt ttccagaaaa tttcccttaa tattatgtcc ctgggcggac
     1021 tggcgctggg ggtcgggatg ctggtggaca actccattgt agtgcttgag agtgtgtttc
     1081 gctgcagaga agaaggagac agtctggtcg atgcggcgga aagaggaacg aaagaagttg
     1141 ccggggcagt gacctcatcc accctgacca ccatctgcgt atttcttccc gtcgcttttg
     1201 tggaaggcat cgccgggcag cttttcggca atttgtcgct gacagtgacc tattctctgc
     1261 tggcttctct tttgacagct ctttatctga ttcctctgat tgtgtcccgg cagggacagg
     1321 aactgcgggc tgaccgctat gttatctggc tggtccgtgc ttatcaggaa gggcgcgcca
     1381 ctgttggcga aagcaagtcc cgggcatttt tttcggttct gccgagagga atccgctatg
     1441 ccggaaccta tattcggaca gtatggcgtg aaagtctgga agtggctttc aaggtgttcg
     1501 gttccgcatt gagaccgccg ctctggaaaa acctgcccca tgcggttttt tatgtagtcc
     1561 tgcttccttt ccttctggcg ctttttctgc tccgtctttt atccggaaca acgatcagcc
     1621 tgctcgttac gggactttat ctgatctgcg ttttctttta tggcattttt agaataatac
     1681 ggggaatatt cggctttgtt ttttatattc cattaaaaag ctttgctctg ggttttgagg
     1741 ctttccgaaa agcctacggt gcgactctgc gtgccttttt gcgattcagt cctgcctttc
     1801 tgctcggcgt agttctgctt tccatccatg ccggatacct tgctctggaa ctgggcagag
     1861 aactgatgcc gcctatgcgt cagggtgaat tcggtctgcg tgtggaggtg cagacgggta
     1921 cacggcttga aaagaccgaa gagcagatgc gccaatttga ggagattctt cgttccactc
     1981 cggaagttgc atcggtgaca ctggaagtgg gttcggaaag agacacggct tcttcagata
     2041 aaggcgacaa cattgccacc tttaccgtac tgctcaaaga agcctcggaa atggaattca
     2101 atcaggatga ggtgatagag cggcttcgcg aaaatatttt tgcgaaaaca accactgaaa
     2161 cagtcacttt tacgctgccg accatgttca gcttccgtaa tgccattgaa acgcagctct
     2221 ttggcgatgt gttgaaagat cttcgcgaat atggcgacaa ggctgttgcg ctcattagca
     2281 ctgttgctgg tgtaaaagat gcggagcaaa gcatcaagcc cggctacccc gaactgatta
     2341 tagagatgga tcgtcaggca cttgcctccc ggggactctc tcccggcaag gtggcacagc
     2401 ggcttcgtac tgaaattcag ggcgaagtgg caacccggtt cagccgggga tcggacaaac
     2461 tggatattcg tgtccgtgcc agccagaaaa aattgagcag tgtggaagat ctgcgatttc
     2521 tttccgtgac ggatggtccg gcacccatcc cgctgattaa tgttgccaaa ctgcgtatcg
     2581 aagaaggtcc cagtgaaatc aggcgtgtca atcagaaacg taccgtgatt ttgtcggcga
     2641 atgtggaggg acgggatctt ggcgcggtgt cgcgtgacat cgatagcacc ttggagaaga
     2701 tggcgctgcc acagaatatg tatttcctgc aaggcgggca aagccgcgag ttggatgccg
     2761 cctttaagag cctccgtttt gtccttcttc tcgccgcctt tcttgtgtat gtggttatgg
     2821 cgtgtcagtt tgaatccatt attcagcctg cccttgtgat gttcagcgtg cctttggctt
     2881 ttatcggagt ggtgtataca ctgtatctga cagggacaaa cttgaatgtg atggtctttc
     2941 ttggcggcat tgtacttatt ggtattgttg tgaataacgc cattattctg gtggattata
     3001 ccaatcagtt gcaggcgcgc ggtctgaata ttaccgatgc gattgtggag gcgggtgtgg
     3061 cgcgtattcg tcccattttt atgaccacta ttactaccgt cctcgggctg acacctatgc
     3121 tgattgcttc cggagaaggg gcggagatgc gccgtcctat ggcactcacc ctcattgccg
     3181 gtctttcttt ttccacactg cttaccctct tcatcattcc tatggcctat tacctgtttg
     3241 gtcggaaaaa aagcctatga agtcgtctct ggctgcattg gcattaaaac ggcccatcat
     3301 ggtgaccatg ctcacaatca ccatggtcgg tttggggatt atttcctggt acaggctgcc
     3361 gttgaatttc ctgccgcgtg cggaatctcc ctttatggtg tgtatcttcc cttatccggg
     3421 tgccgctccg tctcaggtgg aacagcaggt tgccattcct gtggaaggtg agttcagaac
     3481 cctgtcaggt ctgacccgtc tgcgcaccaa ttctacagac agcgcctgtt atgcggaaat
     3541 gaatttcagc cttgaaacag atctgaacca ggtgtcggct gaaattagag accgtattga
     3601 acgcctcaaa cttgttttgc ccaaagaagt tgaaaaagta caattgcagc gttttaatgt
     3661 cgacgcattt cctgtgatgg cggtgggttt attcagtcat ggggatcgcg aggaatttac
     3721 acataaagtc agaacaattg ttgaaccgcg cctgcgccgg cttgacggcg tggccagcat
     3781 tcaggtgcat tcacctaccc agccgcggga ggtgctgatt gaatttgaac aggacaccct
     3841 cactggcctg aatctgtcca tgcctgagat tatccaatac ctacagatcg gcacaatgac
     3901 catttcctta ggcgaattga cagacgatgc tatgcgctat atcgttcgct acgaagggga
     3961 gtaccgttct attgaagcga ttgcggatct gcccataggc aagcagggac ttcacctgcg
     4021 tgatatcgcc acagtacggt tcagttcgcg aagcaaagat ttgcgtgcca ccatggacgg
     4081 tgcggacggg ctggtgcttt ttattactaa agaatctcag gccaatacag tggatacctg
     4141 ccggtctgtc cgtgaggaac tggacaaact gctgctgaca cctgcttttt ccggtgccag
     4201 agtacttccg ctttttgatc agtcggaatt tatagtcaaa gcgctgaata acctgttcct
     4261 tcaggggctt tatggcagca tcatggcagt gggagtgctc tttgttttca tgcaccgttt
     4321 tctgccgacg gcgctggtgg cactgtccat tccttcctct cttgtttttg ctctggtctt
     4381 tatgtttttt acaggcatgt cgctcaatat tattaccatg gtatccatga ttgtggcggt
     4441 gggcatgctc gtcgacaatg ccattgtagt ggtggaaaat attctgcgtc atcgaagtct
     4501 cggtctgtcg attcgtgaag cggcggtgaa agggcctcag gaagtgagcc tggcaattgt
     4561 tgcctccaca gtaacgacct gggttgtgtt tctgcccatg ttttacatga agaccggcca
     4621 gatgtccgtt tttatggaac agctgggcga accgctcatt gccgcattgg gcggcagcct
     4681 gttgggagcg ctcacactca tacccctgct catggtgggc gtggagtttc tggaaagcag
     4741 ggtaaaacgc aggactgcaa aaatacagtg gggttcctgg accaagctgc ccggaaaaat
     4801 tgtggatgcc tgcgtcgatg cctacggcgt tgccttgcgc cattcgctgc ggcagcgttt
     4861 cgtcgtaatt ctgcttgtgc ttctggctgt tctttttaca gcactgtttc ctatgaaaac
     4921 agttggaatg cgcacccttc caaaactgga tatgcgtgag gtgacggtgg atgtctcgct
     4981 cgcacgcaat ttcggtatgg atcgtgccgt ggaactattc aagagtcttg aagcggagat
     5041 tgaccagctg cgggaagtgc ttgcagtaaa atacgtcctc gcctatcatg gcgcgacagg
     5101 cggcgcaatt catgtatatt tgtatacgga agatgatggg cctataggag aggatccgcc
     5161 g
//
LOCUS       DAOPWB010000183         5134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_16271_length_5134_cov_5.657807, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000183 DAOPWB010000000
VERSION     DAOPWB010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16271_length_5134_cov_5.657807"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PKN92_12265"
     CDS             complement(<1..1297)
                     /locus_tag="PKN92_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNZ49608.1"
                     /translation="MPDVFLLLPAAPCRIRRQPGMLLFLLCVMLSPFLSGFSPAAAQE
                     DSGAAPVLIAEPINPDFLKWLETAESVTHGGYVPALFDYHNDPGQSQKAVLPARFDLR
                     DSGAVSPVKNQGTCGSCWAFAACSALEGCMKKQWGFFPDLSENHLIHTHGFLWPPCGG
                     GNNEMALAYLARWGGPYLETDDPYTPGTGSHLREGARVQALLLQASMFSASYGQISLL
                     KQYLYEHGALATSMMWADAFYNSAENGYYAAGSAGEGHGITLIGWDDTKWIAGAPASG
                     AWICKNSWGAGWGDNGSFYLSFYDGGSVKSCMGIDKLAQPENYGTLYSYDPFGVVLKI
                     GYAANAPAFGANVFTAEKDEKISALATLAIADNTVYTAAVYEGAIRADGTTGALLAET
                     SGIFERAGYHTITLPHPVSVSAGQPFTVVVSWKNSTISTV"
     gene            complement(1330..2361)
                     /locus_tag="PKN92_12270"
     CDS             complement(1330..2361)
                     /locus_tag="PKN92_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNZ49609.1"
                     /translation="MTKSNAKALSDPTAYAYCLAPTPWMTALRQRPPLTERHPELTAK
                     RIQIEKRFRSLLPGQSETDPVTLGRGILMAVLRLGLKLLRLYEKGRKNALDLRLNEEE
                     LFFQNLPPGLEGVRILHLSDLHLPRRFPAFAEKAAALLDGLTVDLCVMTGDYRWGYFG
                     PVDHAPEQISTILSGVRSRYGVVGVFGNHDSFIMGETLEKEGIPLLFNEGIALPFGAD
                     TLWVGGIDDPHIFRCTDLKAATAGAPENAWKLLLAHSPEKVREAEALGFSLYLCGHTH
                     GGQIRLPVIGALSYNASCRRSQILGWWQYKNMQGYTSAGLGTTDLPVRYLCPPEATLF
                     TLRKAPKLS"
     gene            2704..4584
                     /locus_tag="PKN92_12275"
     CDS             2704..4584
                     /locus_tag="PKN92_12275"
                     /inference="COORDINATES: protein motif:HMM:NF017180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family
                     metallo-endopeptidase-related protein"
                     /protein_id="HNZ49610.1"
                     /translation="MKKRIILLAVFFSFSVLFSGAALAAYSGGDGTEETPFQLSTATD
                     WVALIATTADWDKHFILTADLDFSGIEVVPIGDSSTPFTGAVDGGGHAMKNIVMTALS
                     SSLFSSLSNATIQNLRIENITVDAENEGAALSLFASKSTFSDCSVSGSISTRNPAGTA
                     AGLVIRAEDCTITHCTSTAEVSAFRASGLAGTCVVCIVTNCATSGKITASGDNAHAGG
                     LLDLCGANTQITNCVSTAEVTVSGKMSFAGGLISDCGSSTLGSCYFKGRVSGDGENLR
                     VGGLAGNAAVSSIMLCSSKAEISTSGDKSSVGGLIGVAVSAVMLQLCTSFSKITSTGE
                     GQIVGGMAGFTGNTNFQSCFFSGEAHIQTGSGIVGGLVGYGMNSALINSYSKGIVDEA
                     QQNGVAGGLAGVLYSDDPAGVVRCAAVTDVRGYTAGGLVGLSIQYNYINCYALGTVSG
                     KSCAGGLIGSVFPTYQPAEVSYCYAAALVEVAEEDGLAGGLIGENENGQVTSSYWDTE
                     VSGISVSAGGEGRTTAEMTWPYAENCYVGWDFEEVWGTDPETEKQGYPQLLSSPSTLY
                     ETEGENEGEGEQPVTPDKQEGCGCSPKDLQTVTKALLADWLLIALCLGALLSGSAVRG
                     KF"
     gene            complement(4555..>5134)
                     /locus_tag="PKN92_12280"
     CDS             complement(4555..>5134)
                     /locus_tag="PKN92_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49611.1"
                     /translation="ALDAGSWFDASFAGTQIPTLKEVLEIIPETILCNVHLKNAPGVA
                     KAAATLIKDMGRLSHCFLACTVSQMKEARAVAPEIMICNMDRQGGDRKAYINLTIEEG
                     CQFIQLTRTQGTDGLKDDVKRLHDAGVRVNWFSAEEEPLMTLLADAGVDYILTDRLVL
                     LQQVLKNRGGAPAESLKCSCKRVSEFSPDSTA"
BASE COUNT         1162 a         1279 c         1464 g         1229 t
ORIGIN      
        1 tgaccgtcga aatggtgctg tttttccacg agacaactac ggtgaatggc tgccctgccg
       61 atacggaaac aggatgcggc agcgtgatgg tatggtatcc tgcgcgctca aaaatgccgc
      121 tggtctcagc cagaagtgcg ccggttgtcc cgtcggcgcg aatggcacct tcgtatactg
      181 ccgccgtgta aacggtgttg tcggcaatgg caagagtagc gagtgcgcta atcttctcgt
      241 ccttttccgc agtaaaaaca ttggcgccga aggcgggcgc attcgcggca tagccgattt
      301 ttaatacaac accgaaggga tcgtaactgt agagtgttcc gtagttttcg ggctgtgcga
      361 gtttatctat gcccatgcaa cttttcacgg aacctccatc gtaaaaggaa agataaaaag
      421 atccgttgtc gccccagcct gctccccagc tgtttttgca gatccaggcg ccggatgcgg
      481 gggctccggc aatccatttg gtgtcgtccc agccgatcag cgtgatgcca tgcccttcac
      541 ctgcactgcc tgcggcgtag tatccgtttt ctgcgctgtt gtaaaacgca tccgcccaca
      601 tcatactggt cgccagggcg ccgtgttcat agagatactg tttcagaaga gagatctgac
      661 catatgacgc actgaacatg gaagcctgca gaaggagtgc ctgaacccgg gcgccctctc
      721 tcagatggga tccggtgccc ggcgtatagg ggtcgtccgt ttccagatag gggccgcccc
      781 accgtgcgag ataggcgagc gccatttcgt tgttgccgcc gccgcaggga ggccagagaa
      841 agccgtgggt gtggatcagg tggttttcag agaggtcggg aaagaagccc cattgttttt
      901 tcatgcagcc ttccagtgcg ctgcaggcgg cgaaagccca gcaggaaccg caggttccct
      961 gatttttaac cggactcacg gctccgctgt cccgaaggtc aaagcgggcg ggcagcaccg
     1021 ctttctgcga ctgcccgggg tcattgtgat agtcgaaaag tgcagggacg tacccgccgt
     1081 gcgttactga ttccgctgtt tccagccatt taagaaagtc aggattgatg ggctctgcga
     1141 tcaggacagg cgccgcgccg ctgtcttcct gtgccgctgc cgggctgaag ccggacagga
     1201 aaggggagag catcacacag agtaaaaaca gcagcatacc gggttggcgg cgtattctgc
     1261 acggagcagc cggaagcaga aggaatacat cgggcatctt ttgtctccga ggggactttt
     1321 tcagtttgtt catgacagtt tcggcgcttt tcgaagtgtg aacagcgtcg cttcaggggg
     1381 gcagagatag cggacaggca gatcggtggt gcccagaccc gcactggtat agccctgcat
     1441 atttttatac tgccaccagc cgagaatctg gctgcggcga caggaagcat tataactgag
     1501 cgcaccgata acaggcaggc ggatctgacc gccgtgcgtg tgaccgcata gataaagtga
     1561 gaaacccagc gcttccgctt cacgcacctt ctccggggaa tgagcgagga gcagcttcca
     1621 ggcattttcg ggtgcgcctg ctgtcgctgc tttcaaatcg gtacagcgga atatgtgggg
     1681 atcgtctatg ccgccgaccc aaagagtgtc ggcaccgaag gggagtgcga tgccttcgtt
     1741 aaataataaa ggaatgccct ctttttccag agtctctccc ataataaaag agtcgtggtt
     1801 gccgaagacg cctaccactc cgtaccggga gcgaacaccg gatagaatgg tactgatctg
     1861 ttccggtgca tggtccacgg gaccgaagta gccccagcgg taatccccgg tcatgacaca
     1921 gagatcaacg gtcagtccat caagcagggc tgccgctttt tccgcaaagg cggggaaacg
     1981 gcgcggcagg tgcagatcag acaggtgcag aatacggacg ccttcgagcc ctggcggcag
     2041 attctgaaag aagagctcct cttcgttcaa gcgcagatca agcgcatttt ttcgcccttt
     2101 ttcataaaga cggagcagct tcagacccag acgaagcact gccatgagaa tgcccctgcc
     2161 cagtgtaaca ggatctgttt cactctggcc cggcagtagc gatctgaagc gtttttcgat
     2221 ctgaatgcgc ttagctgtaa gttccggatg ccgctccgtg agaggcgggc gctggcgcag
     2281 tgctgtcatc cagggggtcg gtgccagaca atacgcatac gccgtcggat cggacagggc
     2341 ttttgcgttg gattttgtca tatcagagag ccttgtagag atctttttac tgccacacag
     2401 gaaagcgcta ttgtatctgt cccggggtat caggctcaaa gccgatttct tctccccagt
     2461 ttgaacaaca ggcacctttg tttttttcga agcatcctgc tcagtttctg acagagaggc
     2521 cccctgtttt aattggaata accataatgc gtagtttgta caaatactgt tctgttactt
     2581 tttataatac aacgaaatct attttaattt tccggtgttt atgactaggc aggttcattg
     2641 atttaagaaa tgattttgag cagttccatg aggaaacgcc ctgatcgaaa aaggaattta
     2701 ccgatgaaaa agcgaataat tcttctggct gtattttttt cgttttctgt ccttttttca
     2761 ggggctgctc tggctgccta cagtggtggt gacggtaccg aagagacacc tttccaattg
     2821 agtactgcaa cggactgggt agcgctcatc gccaccactg ccgactggga taaacatttt
     2881 atcctgaccg ctgatctgga tttcagcggc attgaagtag ttcctatcgg agattccagt
     2941 actcctttta cgggtgctgt ggacgggggc gggcatgcga tgaaaaatat tgtgatgaca
     3001 gccctgagtt catcgctttt ttccagtctg tcgaatgcga caattcaaaa tctccggata
     3061 gagaatatta cagtagacgc ggaaaatgaa ggagcggctt tgtctctttt tgcatccaaa
     3121 agtactttca gcgactgcag tgtttcaggc agtatttcca cgcggaaccc cgccggcaca
     3181 gcagcgggat tggtgataag ggccgaggat tgtaccatta cgcactgtac ctccacggcg
     3241 gaagtaagcg cttttcgtgc cagtggcctt gcgggcactt gtgtagtttg catcgttacc
     3301 aactgtgcaa cttcgggaaa aattacggca tcgggagaca acgctcacgc aggaggactc
     3361 ctggatttat gtggagctaa tactcagata acgaactgtg tttccacagc agaagtaacc
     3421 gtttcaggca aaatgagttt tgctggcggg ctcataagcg actgtggctc gtccacactc
     3481 ggaagttgct attttaaagg cagagtgagt ggagacggtg aaaacctcag ggtaggcgga
     3541 ttggccggta acgctgcagt gagctccatt atgctctgct cttctaaggc agaaatatcc
     3601 acttccggtg acaaatcgag tgtgggtggt ctgatcggcg tggctgtatc ggccgttatg
     3661 ttgcagttat gtacgtcctt ctcgaagatc acgtccacag gagaagggca gatagttggc
     3721 ggcatggccg gttttaccgg caatacgaat tttcagtcct gtttcttttc cggagaggcg
     3781 catatacaga ccgggagtgg tatagtcggt ggtctggtag gatatggcat gaacagcgct
     3841 cttataaaca gctattcgaa aggtatcgtt gatgaagcac agcagaacgg agtggcaggc
     3901 ggtctggcag gtgtgcttta ttcagacgac cccgcggggg tggtgaggtg cgcagcagta
     3961 acggacgtgc gaggctatac tgcaggcggt ctggtcggac tttctataca atacaattac
     4021 atcaattgct atgcgctcgg gacggtatcc ggtaaatcgt gtgcaggcgg tctgatcggc
     4081 tctgtttttc ccacctatca gccggcggaa gtcagctatt gttatgcggc agcgctggta
     4141 gaggttgctg aggaagacgg acttgccggt ggtctgatcg gtgaaaacga aaacggtcaa
     4201 gtcacttctt cttactggga tacggaagtg tcaggaattt cggtcagcgc cggaggtgag
     4261 gggcgtacaa ctgccgaaat gacctggcct tatgccgaaa actgctatgt gggctgggac
     4321 tttgaagaag tatggggaac ggatcccgaa accgaaaaac agggttaccc gcaactcctt
     4381 tcaagcccgt cgaccctgta cgaaaccgaa ggcgaaaatg agggggaagg tgagcagcct
     4441 gttacgccgg ataagcagga gggctgcggc tgttcgccta aagatcttca gactgtgacc
     4501 aaagccttgc ttgccgactg gctgctgatt gccctgtgcc tgggcgcact cctttcaggc
     4561 agtgctgtcc ggggaaaatt ctgagacgcg cttgcatgaa cattttaacg attctgcggg
     4621 tgcaccgcct ctgttcttca gaacctgctg gagcagtacc agacggtctg taagaatata
     4681 atccacgccg gcatccgcaa gcagtgtcat aagcggctct tcttcggcgc tgaaccagtt
     4741 cacccggacg cctgcatcat gaagccgctt aacatcgtct ttaagaccgt cggtgccctg
     4801 tgtgcgggtc agctgaataa actgacagcc ttcttcaatg gtcagattga tataggcttt
     4861 ccggtctccg ccctgccgat ccatattgca gatcatgatc tcgggtgcga cggcacgggc
     4921 ttccttcatc tggctgacgg tgcaggcaag aaaacagtgg ctcagccgtc ccatatcttt
     4981 gatcaaggtg gctgccgcct ttgctacgcc gggcgcattt ttcagatgaa cattgcaaag
     5041 aatggtttcg ggaatgattt ccagcacttc ttttaaagta gggatttgcg taccggcaaa
     5101 agatgcatca aaccagcttc ctgcatcgag ggcg
//
LOCUS       DAOPWB010000184         5080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_16509_length_5080_cov_4.609353, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000184 DAOPWB010000000
VERSION     DAOPWB010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16509_length_5080_cov_4.609353"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKN92_12285"
     CDS             <1..636
                     /locus_tag="PKN92_12285"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HNZ49612.1"
                     /translation="QAEKAGAVFLPLAGDISDLSGHEALVQSVLEHFGHIDLLVNNAG
                     VAPLERRELLAMEPGSFDRVLGINLKGPFFFTQRIASVMKKQVEQKKGHTPRIVFVSS
                     ISSRIVSTNRAEYCISKAGLSMAAQCFAAALAHAGIPVFDLQPGLIATDMTSAVQSKY
                     DALIEEGLVPAGRWGYPDDVGRAVVALAQGHFDFSTGSVIEIGGGIGIPRL"
     gene            859..2148
                     /locus_tag="PKN92_12290"
     CDS             859..2148
                     /locus_tag="PKN92_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ49613.1"
                     /translation="MFRVTDFFICLSVFVCSISSLAYGADWPWYMGPDRTGISGEENL
                     LTLFPAEGPKELWAVSVGAGYGGPSVQDGEVYVLDREDEKRDVLRCLDLDTGKEKWRY
                     SYDAPGSTGHSGSRTAPTVDENNVYSVGLMGDLLCVSRTTHEAVWQHNLLKEYKIALP
                     DWGISQAPFLYEDKIIVAPQAAQAFVVAYSRNTGDVLWEARGEGALGYSSPRVVTLCG
                     EDQVVMISASENRGIGRVLGYSVEDGSLLWSWDGWQCQIPIPWATPLPDDRLFITGGY
                     KAGSVMIQVEKGEKGYSVRELWRLDMNSCGSQIHPPIFYKDHLYVGSNSNEKELGLMC
                     VDLNGKVLWKTKDDRSLPQFERGGFMLAGKTMIALDGKKGTLHLVDPLPFGYKERSRA
                     QVLDGSKMWAPLALSDGRLIVRSQDVMKCPDLRDTAH"
     gene            2196..3647
                     /locus_tag="PKN92_12295"
     CDS             2196..3647
                     /locus_tag="PKN92_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ49614.1"
                     /translation="MFFRVLQQCTKLIILTVSLSVSGCLLYGWMAQPPAVNITVSMPG
                     GDGRPEGASGGEKATDLKGVFVHYAELENRLPGSWTRFRGEKFDNICRDCPPLASRWR
                     EEGPPELWAVDLGEGYAAPVVHDGRVYVLDYDEERHGDLVRCFSLDTGQELWQRFYEV
                     SVKRNHGMSRTVPAVTDSLLVTIGPRCHVLCLDPQTGDYRWGIDLQQEFGATEPLWYT
                     GQCPLIDDGVVVLAAGGPEALMLSVDGMSGEVLWKTPNPQNREMSHSSVMPLHLAGTA
                     TYVYSALGAVVGVAAEGEKRGELLWEVPWKARVIAPSAVQVDEERFLVTAGYGEGSLM
                     IRVQQHGGKYEAEVLYAVTPKEGIACEQQTPVLSEGLLYTVMPKDAAALREQFACYNA
                     DGTLVWSSGSENRFGLGPFILADDKFFILSDDGVLTMAALSKEKFERLDSRRVLNGND
                     AWGPIAIAGTRMLLRDSKKLICLDMGVEANKRG"
     gene            3647..4663
                     /locus_tag="PKN92_12300"
     CDS             3647..4663
                     /locus_tag="PKN92_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49615.1"
                     /translation="METNETKSLFPWISGAAVILLGVTVVALFMSDHRPEPGTAFRFD
                     VAGFQERSKADGIYEETGNLPLPLERPTAAAPGAEGTFFAAARDKIIHLNPDGSVLQE
                     FAVSGVPRCLVSGNENRLYAGVDGHIEVWSLGGDLLESWQPAGEKSWLTSLLLHEENL
                     YSADAGLRCVLRYDLSGALLQRIGEKDAGRDIPGLIVPTPFLDIAVDPLGAFWTTNPG
                     RHGLESYRPDGELLSAWYRPAMGTAGFCGCSNPAHIAFLPDGTLVTAEHGLSRVKLYS
                     PDQQFLMMVAGPETFGESPGMMLPQDQEPAIRDLLVDEKGRILVLDAKKAQLRIFEKK
                     KGMP"
     gene            4660..4857
                     /locus_tag="PKN92_12305"
     CDS             4660..4857
                     /locus_tag="PKN92_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49616.1"
                     /translation="MSDKALSRYDFFHALVRWALGTGLAALVAWLWRQGQLTRTCAGS
                     CAGCMINQWCAQRRDNEENRL"
     gene            4854..>5080
                     /locus_tag="PKN92_12310"
     CDS             4854..>5080
                     /locus_tag="PKN92_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNZ49617.1"
                     /translation="MSCLNRRDFLRAAAATVGSGIALQAVQPGAEATVWQIDPALCVQ
                     CGRCATNCVINPSAIKYIHSYPICGYCDLCGA"
BASE COUNT         1090 a         1219 c         1555 g         1216 t
ORIGIN      
        1 caggcggaga aagctggtgc agttttcctg cctctcgccg gggatatttc cgatctttca
       61 ggacatgaag cgctggtgca gtcggtgctg gagcatttcg ggcatattga cctgctggtg
      121 aacaatgccg gggtggcgcc tcttgaacgt cgggaactgc ttgccatgga gcccggcagt
      181 tttgatcgtg tgctgggcat taatctcaaa ggcccctttt ttttcacaca gcgcattgct
      241 tcggtgatga aaaaacaggt tgagcagaaa aaagggcata ccccccgaat agtgtttgtt
      301 tccagcattt cttcgcggat tgtgagcacc aaccgggcgg aatactgcat cagcaaagca
      361 gggctgagca tggcggcgca gtgtttcgcc gctgccctcg ctcatgcggg cataccggtc
      421 tttgatctgc agccggggct tatcgccacg gacatgacgt ccgctgtgca gtctaaatac
      481 gatgcgctta tcgaagaggg gctggtgcct gccgggcgct ggggctatcc tgatgatgtg
      541 ggcagggcag tggttgctct ggcacagggt cattttgact tcagcacggg ctcagtaata
      601 gaaatcggcg gcggcatagg gatacctcgc ttataaaaat acctgcctga tcctgtaatg
      661 gaagatacct ttttcgggag tctctcattg tctgcctttc cgctcttgga gtataatcag
      721 cattactaaa caggtgcatg aaagtgtggg gcaggatgtg gcaggcgggc tgtttttaca
      781 acttgccatg atctgacaaa gaaaattcag ggagctttca ccccgggtca ttgtcattat
      841 atccaaaaag gagttgctat gttcagagta acagattttt ttatatgcct gagtgtcttt
      901 gtgtgttcca tctcatccct ggcatatggt gcagactggc cctggtacat gggccctgac
      961 agaaccggta tttccggtga agagaatttg ctgacacttt ttcctgcaga aggacctaaa
     1021 gaattgtggg ctgtgtctgt cggcgccgga tatggcggtc cttccgtaca ggatggcgaa
     1081 gtgtatgtgc tggatcgcga agacgaaaaa cgggatgttc tccggtgtct cgatctggat
     1141 acaggcaagg aaaaatggcg gtacagttac gatgcacccg gcagcaccgg acacagcggt
     1201 tcccgtaccg caccgactgt tgatgaaaac aatgtataca gcgtcgggct gatgggcgat
     1261 ttactgtgtg tcagccgtac aacccatgaa gcggtctggc agcacaatct gttgaaagaa
     1321 tacaaaattg ctcttccgga ttggggcatc tcgcaggcgc cttttttata tgaagataag
     1381 atcattgtgg cgcctcaggc agctcaggcc tttgtcgtag cctacagcag gaatacaggc
     1441 gatgtgctct gggaggcgcg tggcgaaggc gcactcgggt attcatcgcc ccgggttgtg
     1501 acgctctgcg gcgaagatca ggtcgttatg atcagcgcat cggaaaacag gggcattggc
     1561 agagtactcg gctattccgt tgaagatggc tctcttttat ggtcctggga tggctggcaa
     1621 tgccagatac ccattccctg ggcaacccct ctgccggatg accggctctt tattacaggg
     1681 ggatataaag cagggtccgt gatgatccag gtagaaaaag gagagaaagg atatagtgtc
     1741 cgggagctgt ggcgtcttga tatgaacagc tgcggcagcc agattcaccc gcccattttt
     1801 tacaaagacc acctttatgt gggcagcaat tcaaatgaga aagaactggg acttatgtgc
     1861 gttgatctga acggaaaagt cctgtggaaa acaaaagatg accggagcct gcctcaattt
     1921 gaacggggcg gctttatgct tgcaggtaaa acaatgattg ctttggatgg taaaaaaggg
     1981 acgcttcatc ttgttgatcc tctgcccttt ggctacaagg agcggtcgcg ggcgcaggtg
     2041 ctggatggca gcaaaatgtg ggcgcccctt gccctttcgg acgggcggct gattgttcgc
     2101 agtcaggatg ttatgaaatg tccggatctg cgcgacaccg cccattgaat ttgcgccgga
     2161 cggcagtgac ggatttttcc ggaaagcagg tgtaagtgtt ttttagagtc ctccagcaat
     2221 gcacaaaact tattattctg acggtcagtt tgtctgtttc cggctgtctt ctgtatggat
     2281 ggatggcgca gccgcctgcc gtgaatatta ctgtgagtat gccgggcgga gacgggcgcc
     2341 ccgagggagc gagcgggggc gagaaagcga ctgatctgaa aggcgtgttc gtgcattacg
     2401 ccgaactgga aaacagactg cccggcagct ggacacggtt tcggggagag aaattcgaca
     2461 acatatgccg ggactgcccg ccgctggctt cccgctggcg cgaggaaggc ccccccgaat
     2521 tgtgggccgt tgacctggga gaaggctatg cggcaccggt agttcacgac ggacgggtct
     2581 acgtgctgga ctatgatgaa gagcggcatg gtgatctggt gcgctgtttt tcgctggaca
     2641 cgggacagga actgtggcag cgtttttatg aggtttctgt taagcgtaac cacggtatgt
     2701 ccagaacggt tcctgcagta acggattcgc ttcttgtgac gataggaccg cggtgccatg
     2761 tgctgtgtct ggatccgcag acgggcgatt accggtgggg cattgatttg cagcaggaat
     2821 tcggtgccac cgagcctctc tggtatacgg ggcagtgccc gcttattgat gacggtgttg
     2881 tcgtgcttgc agcgggcggt cccgaggcgc ttatgcttag cgtggacggc atgagcggag
     2941 aggtgttgtg gaagacaccc aatccccaga atagagaaat gtcgcattct tccgttatgc
     3001 cccttcatct ggccggtacc gccacctacg tttacagtgc gctcggtgcg gtcgtaggcg
     3061 ttgcggcgga gggagaaaag cggggagaac ttctctggga agtgccctgg aaagcccggg
     3121 tgattgcgcc gtcggcagtg caggtggacg aagagcgctt cctggtaacg gcaggctacg
     3181 gagagggcag cctgatgatc cgggtgcagc agcacggcgg gaagtatgag gcagaagtgc
     3241 tgtatgccgt cacgcccaaa gagggtatcg cctgcgaaca gcagacaccg gttctgtctg
     3301 aggggctttt gtatacggtg atgcccaagg atgccgccgc actcagagaa cagtttgcct
     3361 gttacaatgc ggatggaact cttgtctggt cgagcggttc cgagaatcgt ttcggtctcg
     3421 gtccttttat tcttgcggac gacaagttct ttattttaag cgatgacggt gtgctgacca
     3481 tggcggcatt gagcaaagag aaatttgagc gtctggattc ccgacgtgtt cttaacggaa
     3541 atgacgcctg gggtcccatt gccattgcgg gcaccagaat gcttctcaga gattcaaaaa
     3601 aactgatatg cctggatatg ggcgtggaag cgaataaaag aggatgatgg aaactaacga
     3661 aactaaaagt ctctttccct ggatttcagg agcggctgtc atcctgcttg gagtgacggt
     3721 agtggcgctg ttcatgtctg atcaccgccc tgagccgggt acagcctttc gctttgacgt
     3781 tgccggtttt caggagcgat ccaaagcgga tggtatctat gaagaaaccg ggaatctccc
     3841 gcttccttta gaaaggccta cagcggctgc tcctggtgcc gaagggactt tttttgccgc
     3901 tgcaagagat aaaataattc acttgaatcc ggacggcagt gtgctgcagg aatttgccgt
     3961 atccggcgtg ccccgttgcc tcgtatccgg gaatgaaaac agactgtatg ccggagtgga
     4021 tggtcacatt gaagtctgga gccttggcgg ggatttgctg gagagctggc agcctgccgg
     4081 agaaaaatcc tggcttacgt cgctgctgct tcatgaagaa aatttgtact ctgccgatgc
     4141 cggactgcgt tgcgtcctgc ggtatgatct ttcgggtgca cttttgcagc gcatcggaga
     4201 aaaagatgcc gggcgggata ttcccgggct tatcgtaccc accccctttc tcgatattgc
     4261 cgtcgatcct ctgggggcgt tctggactac caatccgggc agacacgggc ttgaatcgta
     4321 tcgcccggac ggagagttgt tgagtgcctg gtatcggccc gccatgggca cagcgggctt
     4381 ttgcggatgc agcaatccgg cgcatatcgc ctttttgccg gacggtactc ttgttacagc
     4441 ggaacatggg ctttcccggg tgaaactcta cagcccggat cagcagtttc tcatgatggt
     4501 tgccggaccg gaaacttttg gcgaaagccc gggcatgatg cttccacagg atcaggagcc
     4561 cgcaattcgc gatctgcttg tggacgaaaa agggcgcatc ctggttttgg atgcaaaaaa
     4621 agcgcaactg cgtatctttg agaagaaaaa gggtatgcca tgagcgataa agcgctgtcc
     4681 cggtatgatt tttttcatgc gctcgtgcgc tgggcgctgg gtacaggtct ggctgcactg
     4741 gtggcgtggc tctggcgtca gggacagctg acacggacat gtgccggatc ctgtgccgga
     4801 tgcatgatca atcaatggtg cgcacagcgc cgggacaatg aggagaacag gctgtgagct
     4861 gtctgaaccg tcgtgatttt ctcagagccg ccgcggcaac cgtgggatcc ggcattgcgc
     4921 ttcaggcagt gcagccgggt gccgaagcaa ctgtctggca gatcgatccc gccctgtgcg
     4981 tgcaatgcgg acgctgtgcc accaactgcg tgatcaaccc ttctgctata aaatatatcc
     5041 attcctatcc catctgtggc tattgtgacc tgtgcggtgc
//
LOCUS       DAOPWB010000185         5034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_16719_length_5034_cov_5.536855, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000185 DAOPWB010000000
VERSION     DAOPWB010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16719_length_5034_cov_5.536855"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PKN92_12315"
     CDS             <1..838
                     /locus_tag="PKN92_12315"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNZ49618.1"
                     /translation="LAREFKKYAPSLLVASSENGDVKVTADPAEAGDSGLFFLVNALP
                     ENPWGNVNYLLPAEEATFALLEEAYTNDVEKRPWVPDNSRLSEEERAEGFISLFNGKD
                     LDNWWNYKHDKDSFRVNEEGWIECYQSGAGGLITRDRYADFILRLEYMQREPGTNSGI
                     HLRVPRAARQSKLGFEFQLMADSDLTEPHSTSTGAIYDVLPAQALAVKKEGEWNSVEI
                     ILDGPRYKATLNGIVVQDVNFDDIEELKYRLRRGFIGLQDHHDYVAFRNIRIKVLNAD
                     MP"
     gene            856..1917
                     /gene="mutY"
                     /locus_tag="PKN92_12320"
     CDS             856..1917
                     /gene="mutY"
                     /locus_tag="PKN92_12320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNZ49619.1"
                     /translation="MRHGRTKFHAALLQWYDAEARDLPWRRTQDPYCIWLSEIMMQQT
                     RIDQGTPYYERFLKRFPDLQTLAQATEEAVLKQWEGLGYYSRARNLYRTAQILTDQYH
                     GRFPESPEVLQLLPGIGKYTAGAIASIAFGVPVPVLDGNIKRVLARLYALVESIDLPA
                     TDKRLWELAAATLNVKRPGDHNQAMMELGARVCIPAGPLCFSCPVQRHCRAFSASLQD
                     TLPHRTPKKVVPHHEMVVAVIANQDAYLIGKRPSQGFLGGLWEFPGDRIQKTEGCEDA
                     LRRIGEEMLRLKVVPGGLVAVVRHAYTHFKVTLHVYRCTAVKRRICSDAHTEYRWVTP
                     DSFDQYPFPAGHHKFLKLL"
     gene            complement(1937..3349)
                     /locus_tag="PKN92_12325"
     CDS             complement(1937..3349)
                     /locus_tag="PKN92_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49620.1"
                     /translation="MDTFFSACIALLFTTCSCDLASHITTSPSGHWVEYEGRTLMLVG
                     DSGTQCVLQNANLNYRAWIDDCAERGIPMIHVWSFMAPRQKSDGSELEERWKYVYPGL
                     TPWRRHSGGQPAHDQLPRWNLQLFDDGADGALDHYWPRARDLCAYARSKKILVGYTVF
                     TGWIKGNANAWAYHPFNKVNGGHLDRNLPDGVTIATPGQEIWNKDWQEDWSTAEKTQW
                     IWERFALKAIEELNPFGNVFFVFFDEHSYTEGNMGDHFLQFFKKRNALWMDWAPRRQQ
                     VDFVLNDTNDRPDKNSLSVAGFFAKPARPYFLLEGGPYRGADVRTSMWTFAMGGGHYS
                     YHGDTEQETPQTGIVGYDPLVPGGDKGMERRDWLGHMSRFFNDRIVNLDRMAPHNELV
                     SEGVFCMAAPGQEYALYFTSDSPRNTSLKLPSGSIEATVTFCNPRTGKESAPVAVAVE
                     QELQLSAPDEQDWAVHVLCR"
     gene            3552..5012
                     /locus_tag="PKN92_12330"
     CDS             3552..5012
                     /locus_tag="PKN92_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ49621.1"
                     /translation="MAKLKASSPTGITRRSFIQSASAISLGSLFSIGCARGDGPSVND
                     KINIACIGTGWQGLYNTEVFMQDKNARVVAVCDPFKEGQYMQRGICGREPARRMVNQY
                     YDYSGPEKGGCAGYTDFREMLEERPDIDAVVITAPDHIHAVAALAAIRKGKHVFCEKP
                     LGHSLKEVRMMREEAHAAGVATQMGNWGHGMEDIRRLCEMIWDGAIGAVREVHAWANR
                     PGGWWPYGIDRPEDEPPVPEGMDWDLWLGPAPWRPWHPAYHPFIWRGWWDFGSGALGD
                     MACHILDPVVWSLHLGAPESVEATSVKLNSKATPFGGEMPVWKVHPETTTAGAYLRWK
                     FPERKGMPPVTVIWSDGGLRPTRPDALAPDKPMGDGDGGVLFMGDEGILLCGCYGKDP
                     QLLPASKMEAYTMPKPTLPRSVGHYKEWVLACRGEGTAWSNFDYAGPLTEIVTLGIAA
                     IKADCKLLWDAESMKFPNAPEHEHLITPVMREGWSL"
BASE COUNT         1165 a         1256 c         1400 g         1213 t
ORIGIN      
        1 tcttgcccgg gaattcaaaa aatatgcgcc ttcattactt gtcgcatcat cggagaacgg
       61 tgatgtgaaa gtaacagcgg atccggctga agcgggcgac agcggactgt tttttcttgt
      121 aaatgctttg ccggagaatc cctggggtaa tgtgaattat cttcttcccg cagaggaggc
      181 gacttttgct ttgctcgaag aggcgtatac caacgatgtg gaaaaacggc cctgggtgcc
      241 cgacaacagc cgtctcagcg aagaggaacg ggctgagggt tttatttctc tttttaacgg
      301 aaaagatctg gataactggt ggaattacaa gcacgacaag gattcttttc gtgtaaatga
      361 agagggctgg attgaatgct accagagcgg agcgggaggg ttgatcaccc gtgaccgcta
      421 tgctgatttt attctccgtc ttgaatacat gcagcgtgaa ccgggcacta acagcggtat
      481 tcatctgcgg gttccccgtg ccgcacgcca atccaaactg ggctttgaat ttcagctgat
      541 ggctgactcc gatctgactg agccccacag caccagcacc ggtgccattt acgatgttct
      601 gcctgcgcag gcactggctg taaaaaaaga aggggagtgg aatagtgtcg aaatcatcct
      661 ggatggtccc cgttataaag ctacattgaa tggcattgtt gtgcaggacg tgaatttcga
      721 tgatattgag gaattgaagt accggctgcg ccgtggattt atcggtctgc aggatcatca
      781 tgattatgtg gcattccgga atatccgcat caaagtgctc aatgccgata tgccctgagc
      841 ccccctgatg gttttatgag acacggaaga acaaaatttc atgcagccct gctgcaatgg
      901 tacgatgcag aggcgcgtga tcttccctgg cgccgcacgc aggatcctta ttgcatctgg
      961 ctttcagaaa tcatgatgca gcagacccgc atagatcagg ggactccgta ttatgaacgc
     1021 tttctcaagc gttttcctga tctgcagacc ctggcgcagg caacggaaga agcggtgctg
     1081 aagcagtggg aagggttggg ctattacagc cgtgcccgta atctgtaccg gacagcacaa
     1141 attctgacag accagtatca tggacgtttt cctgaaagtc cggaagtgct gcagctgctg
     1201 ccgggcatcg gaaaatatac cgccggcgcc attgcaagca ttgcttttgg tgttcctgtt
     1261 ccggttcttg acggtaatat caaaagggtt ctcgcccgtt tgtatgctct tgtggagtct
     1321 attgatctgc ctgccacgga caagcgcctg tgggagctgg ctgctgcaac cttgaacgtg
     1381 aagagacccg gcgatcacaa tcaggcaatg atggaattgg gtgcccgcgt ctgcatccct
     1441 gccgggccct tgtgtttcag ttgtccggta cagcggcact gccgtgcatt ttccgccagc
     1501 cttcaggata cactcccgca ccgcaccccc aaaaaagttg taccgcatca tgaaatggtg
     1561 gtggcggtca tagcaaatca ggacgcttat cttatcggca aaaggccgtc tcagggtttt
     1621 ctcggaggac tctgggaatt tcccggcgac cgcattcaga agactgaagg ctgcgaggat
     1681 gctttgcgca gaatagggga ggagatgctg cgtctaaagg tcgtgccggg cggactggtc
     1741 gctgttgtgc gccatgccta cacccatttt aaggtaacgc tccatgtgta ccgatgtacg
     1801 gcggtaaagc ggcgaatctg ttcagatgcg catacggaat accgatgggt gactccggac
     1861 tcttttgatc aatatccttt tccggcagga catcataaat ttctgaagct gctgtagacc
     1921 gctccggtgc ctcaattcat cggcaaagaa catggacagc ccagtcctgt tcatcgggtg
     1981 cggagagctg taattcttgc tcaactgcca ctgcaacagg tgccgattct tttcctgttc
     2041 ggggattgca gaaagtgacc gttgcttcaa tgctgccgga tggcagtttc agggaagtat
     2101 tacgcgggct gtccgatgtg aaatagaggg catattcctg accgggggca gccatgcaga
     2161 aaacaccttc actgacaagc tcgttgtgcg gtgccatccg gtcgagattt acaatcctgt
     2221 cattaaaaaa gcggctcata tgtccgagcc agtcccgtcg ttccatacct ttgtctccgc
     2281 cgggcaccag cggatcgtag ccgacaatac ctgtttgcgg tgtttcctgt tctgtatcgc
     2341 catgataact gtaatgtccg ccgcccatgg caaaagtcca catacttgtg cgaacgtccg
     2401 cacccctata gggacctcct tccagaagga aataagggcg agcaggtttt gcaaaaaagc
     2461 ccgctactga aagactgttt ttgtcgggtc ggtcgtttgt atcattcaat acaaagtcca
     2521 cctgctgccg acgtggcgcc cagtccatcc ataacgcatt tcttttttta aaaaactgaa
     2581 ggaagtgatc acccatattg ccttctgtat aagagtgttc atcgaaaaaa acaaaaaaga
     2641 cattaccgaa ggggttaagt tcctcaatgg ctttcagagc gaaacgttcc cagatccact
     2701 gtgttttttc tgcagtgctc cagtcttcct gccagtcttt gttccagatc tcctgccctg
     2761 gtgtcgcgat ggttactcca tcgggcaaat tgcgatcgag atgaccgcca ttgactttat
     2821 tgaacgggtg gtaggcccag gcgttggcat tgcctttgat ccatccggta aacacggtgt
     2881 aaccgaccag tattttttta cttcgggcat aggcgcaaag atcccgggca cgtggccagt
     2941 aatgatccag tgcgccgtcc gcaccgtcat caaagagttg aagattccaa cggggcagct
     3001 gatcatgtgc gggctgtcca ccagaatgtc gcctccaggg agtcagtccc gggtagacat
     3061 acttccagcg ctcttccagt tcgcttccat ctgacttttg tcggggcgcc ataaaagacc
     3121 acacatggat cataggaatg ccccgttccg cacagtcatc gatccaggca cggtaattca
     3181 gattggcgtt ctgcagtacg cactgtgtcc cgctatcgcc gacgagcatg agcgttctgc
     3241 cttcatattc cacccaatgt ccgctaggac tcgtggtgat atggcttgca aggtcgcagc
     3301 tgcaggtagt gaacagaaga gctatacacg ctgaaaagaa tgtatccaca atgagttcct
     3361 gtactttgat acagttcaaa tgacgccatt atatctgtcg gaaaagaagg gcagtcgcga
     3421 acccgcccaa ttgttgcaaa aatagacgct ccgggagtat ggtaacatag aagtgctcga
     3481 gtgaatgtac gggtgctctg tattttgatg gaggtcaaaa agcggttagt cgctgcaaag
     3541 gaggaatgaa gatggcaaaa ttgaaggctt ccagtccgac cggcattaca cgacgaagtt
     3601 ttatccaaag cgcatcagca atttcgctgg gctctctttt ctccatcggt tgcgcccggg
     3661 gggacgggcc ttctgtcaac gataaaatta atattgcctg catcggcacc ggctggcagg
     3721 ggctgtacaa tacggaagtg ttcatgcagg acaaaaatgc ccgtgtggtg gcagtgtgcg
     3781 atccttttaa agagggtcag tatatgcagc gcggtatttg cggcagagag ccggcccgac
     3841 gcatggtcaa tcaatattat gactacagcg gcccggaaaa agggggctgt gcaggttata
     3901 ccgatttccg ggagatgctg gaagagcgcc cggatattga tgccgttgta attaccgcac
     3961 ccgatcatat tcatgctgtt gctgcgcttg cagccatccg caaggggaaa catgtgtttt
     4021 gtgaaaagcc cctcggccat tctttaaaag aggtgcgcat gatgcgggaa gaggcacatg
     4081 cagccggtgt tgccacgcaa atgggcaact gggggcatgg catggaagat atacggcgcc
     4141 tttgcgaaat gatctgggac ggtgcaatcg gtgctgtccg ggaagtgcat gcctgggcga
     4201 accgcccggg cggctggtgg ccctatggca tcgaccggcc ggaagatgag cctcccgtgc
     4261 ctgaaggcat ggattgggat ctgtggctgg gcccggcacc gtggcgcccc tggcatcctg
     4321 cctatcatcc ctttatctgg cggggctggt gggatttcgg atcgggtgct ctgggcgata
     4381 tggcatgtca tattctcgat cctgtagtct ggtcgcttca tctgggagcg ccggaatcag
     4441 tggaggcaac ttcggtaaaa ctcaattcca aagcaacccc tttcggcgga gaaatgccgg
     4501 tatggaaagt gcatccggaa acaaccacgg caggtgcgta tcttcgctgg aaattccctg
     4561 aacggaaagg gatgccgcct gttactgtca tatggagcga tggcgggctg cggcccacgc
     4621 gacccgatgc tctcgcgccg gataaaccga tgggagacgg ggacggcggt gttctgttta
     4681 tgggcgatga aggcatcctt ttgtgcggtt gctatggcaa agatccgcag cttcttcctg
     4741 cgtctaaaat ggaggcatat acgatgccga agccaacatt gccgcggtcg gtcgggcatt
     4801 ataaagaatg ggttctggcc tgccgcggtg agggaaccgc ctggtccaat tttgattatg
     4861 ccggtccgct gaccgaaatt gtaaccctcg gcattgctgc catcaaagcg gactgcaaac
     4921 tcctctggga tgcggaaagt atgaaatttc ccaatgcacc tgagcacgaa catctcatca
     4981 cgccggtcat gcgtgaagga tggagccttt aataaacgtg aatgcctttt tcag
//
LOCUS       DAOPWB010000186         4951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_17081_length_4951_cov_5.487541, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000186 DAOPWB010000000
VERSION     DAOPWB010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17081_length_4951_cov_5.487541"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PKN92_12335"
     CDS             <1..1128
                     /locus_tag="PKN92_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HNZ49622.1"
                     /translation="FALEGDIDFENAALHPIGSDQQGKAFTGTFDGKKHTVRNGQITV
                     GDKSSAGLFGTLGKGGVLKNISVESFTVTGKMNVGGLVGFAGNGSTIDACSSAADVSG
                     LQNVGGLAGRQEMATIKNSSASGKVTGSSIATGGFLGLSGGSGWASGGTISRCRATGA
                     VTGAQVVGGFVGSVQGGTISHCSASGTVTGKRTVGGFAGGSDDTLTSCFATGAVKNEG
                     PISGGFVGSSSGTISECYSTGNVSGNADTGGLAGKISGRVFNCYALGNAESAVNYSGA
                     LAARMLEQSALENCYAVGTVKGKAFSGGLVGKKSEGSTIQNCYWQQSDADKADESARS
                     KEALTGPQDENTFQGWDFEKTWSADEDQSVNKGYPYLKSTR"
     gene            complement(1327..2049)
                     /locus_tag="PKN92_12340"
     CDS             complement(1327..2049)
                     /locus_tag="PKN92_12340"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNZ49623.1"
                     /translation="MNDSRRNFLKTAGGIAAAGAVLTTVVSEAKAQNEVAGALTEHTL
                     PELPYAYDALEPYIDAKTMELHHSKHHAAYVSGLNAAEAALAKARAEGNFDLVQLYSK
                     RAAFNGGGHFLHSLFWKVMAPAGKGGGGEPSGVLLDAINRDFGSFDAFKKQFSAAALQ
                     VEGSGWGLLLHRPSDGRLVVSQAENQHKLTSWDAFPILGIDVWEHAYYLNYQNRRADY
                     IAAWWNVVNWAQVEANLRHHLV"
     gene            complement(2217..2885)
                     /locus_tag="PKN92_12345"
     CDS             complement(2217..2885)
                     /locus_tag="PKN92_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49624.1"
                     /translation="MILSRRKFMAGMAAAVAAGLTRKGIVMASAEKREITRHPNEYFY
                     KADGTFDEAKAKEAYYKMFEEFGYPIVPRLRSADFWVVDFGLGKFAEVGMGGIFWVNN
                     QEDNYFGHDIYLLPGQMIPEHRHVRTEAAPKMEGWQPRHGWVYIYGEGEPTPGVEERI
                     PPSHKECAIARCEKKLMPGEVGQLGGAEQWHWMYAPEGAIVTEYATYHDGAGLRFSHP
                     DVVF"
     gene            complement(2919..3806)
                     /gene="lsrF"
                     /locus_tag="PKN92_12350"
     CDS             complement(2919..3806)
                     /gene="lsrF"
                     /locus_tag="PKN92_12350"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HNZ49625.1"
                     /translation="MPDADNYADARKEYGVDIPMETPGFFLKGSAHLNWGMKNRLSQI
                     FNPKSGRTVMLAFDHGYIMGPTTGLERLDLVVPPLIPHVDCLMGTRGAFRSCISPEVR
                     KPLVMRCSTGATVLKELSNEVIGVTLEDALAFNAAAVTTQVCIGAEYERETLENLAYL
                     INEGNAFGMPTLGVTAVGKEMVRDARYLGLACRVIAEVGAHFVKTYYCEPGFETVVAG
                     CPIPVVIAGGKKLPEEEALAMAYKAVDQGALGVDMGRNIFGAEDPVAMAQAVGAVVHR
                     LESPKQAFALYNDLKGKCC"
     gene            complement(3799..4440)
                     /gene="dhaL"
                     /locus_tag="PKN92_12355"
     CDS             complement(3799..4440)
                     /gene="dhaL"
                     /locus_tag="PKN92_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HNZ49626.1"
                     /translation="MNTLDYHGLARMFEGAAASVLEKQDYLSRLDSVGGDGDHGTTMA
                     RAMGKVLHAVKSHESQKIATLLQEIGWAVMGVDGGAIGPLLGMMFISMAESAEGLDVL
                     DGEALACAFEAGLAGVHAQTKAQPGDKTLLDALIPAVEALSASARAGESIAAALYCAA
                     EAAEAGAEFTKTLQARFGRARNIGEQSIGSADPGATSIALIFKGFMKGITDHA"
     gene            complement(4447..>4951)
                     /locus_tag="PKN92_12360"
     CDS             complement(4447..>4951)
                     /locus_tag="PKN92_12360"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HNZ49627.1"
                     /translation="EAGRSLEECAEIARRMEAGMATLAVAVSGATHPSTGEVIAAIDE
                     GTMVVGMGQHGEAGGGTQSLKSADETAEIMLNALLDDIDAKAGDELFVLLNGAGATTL
                     MELYLVLRRVNQLLADKGITLARSLVGEYLTVQEMGGFQMCIGKLDEELKALWDAPCD
                     APAWTRK"
BASE COUNT         1124 a         1462 c         1294 g         1071 t
ORIGIN      
        1 tttgccctgg aaggggatat tgacttcgaa aacgcggcgc tccaccccat cggcagcgat
       61 cagcagggaa aagcctttac aggcactttc gacggtaaaa agcacacggt acgcaacggg
      121 cagattaccg tcggtgacaa aagcagcgca ggcctctttg gtaccctcgg aaaaggcggc
      181 gtacttaaaa atatatctgt ggagtctttc actgtcaccg gaaaaatgaa tgtgggcgga
      241 ctggtcggct ttgccggaaa cggcagcact attgacgcct gctcttccgc ggctgacgtg
      301 tccgggttgc agaatgtggg cggactggcg ggcaggcagg aaatggcgac cattaaaaac
      361 agcagcgctt cagggaaagt gacaggaagc agcatagcaa caggcggttt tctcggactc
      421 tccggcggaa gcggatgggc ttcgggcggc actatttcca gatgccgtgc cacaggcgcc
      481 gttaccggcg ctcaggttgt gggcggcttt gtcggctcgg ttcagggagg caccatctct
      541 cactgctccg cttccggcac tgtgaccgga aaaagaacgg tcggcggttt tgcgggcggc
      601 tctgacgaca cactgacttc ctgctttgcc acaggcgctg taaaaaatga aggacctatt
      661 tcaggcggat tcgtgggcag cagcagcggc accatcagcg aatgctacag cacgggcaac
      721 gtcagcggca atgctgatac cggcggtctg gcaggcaaaa tcagcggaag agtattcaac
      781 tgctatgcgc tgggcaacgc tgaatcggcg gtcaattaca gcggagcact ggcagccagg
      841 atgctggagc agagtgctct ggaaaactgc tatgccgtcg gaaccgtgaa gggcaaagcc
      901 ttttccggcg gactcgtcgg caaaaaaagt gaaggctcca ccattcaaaa ctgctattgg
      961 cagcagagcg atgccgataa agccgacgaa tcggcacgca gcaaagaggc gctgacaggc
     1021 cctcaggacg aaaacacctt tcagggctgg gatttcgaaa aaacctggag cgccgatgaa
     1081 gatcagagtg tcaacaaggg ttatccctac ctgaagagca cacgctgagc aggacgggct
     1141 caactgcccg ggcatgaagc agaatttata acgctgttcc tcttttgcgg gagcagcgtt
     1201 tttctttgcc acggcttacc ggcctcggct atatacttca gcgtcaaaaa aaaaggaaag
     1261 ggacagcagg tattagactg tccctttgag tgaggtttcc aaaggagtgg ttatgtcaga
     1321 gacacatcaa acaagatgat gccgaagatt tgcttctacc tgtgcccaat tgaccacatt
     1381 ccaccaggcg gcgatatagt cggcgcgccg gttctggtag ttcaggtaat aggcatgttc
     1441 ccatacgtca atgcccagaa tgggaaaagc gtcccaactg gtcagtttat gctggttctc
     1501 cgcttgcgaa acgaccaggc gaccgtcgga aggccggtgc aggagaagcc cccagccact
     1561 gccttccacc tgcaacgctg cagcggaaaa ctgcttttta aaggcatcaa agctgccgaa
     1621 atcgcggttg atggcatcca gcagcacacc ggaaggctca ccgcctcccc cttttcctgc
     1681 aggtgccatc actttccaga aaaggctgtg caggaaatgc ccgcctccgt tgaaagcggc
     1741 gcgtttgctg tacagctgca caagatcaaa gttgccctct gcacgagctt tcgccaaagc
     1801 ggcctcggcg gcattcagac cgctcacata cgccgcatgg tgcttgctat ggtgcagttc
     1861 catcgttttt gcatcaatat agggctcgag cgcgtcataa gcataaggca gttcgggcag
     1921 agtatgctcg gtgagggcgc ctgccacttc gttctgcgct tttgcttctg atacgactgt
     1981 ggttaaaaca gcaccggcag cggcaatacc gccggccgtt ttcaaaaaat tccgtcttga
     2041 atcgttcatt tttcctccgt ttaattcttt ggttcacgtg tgttccatag agagaaccca
     2101 agccggttaa tttatattcc tgaacatttg ttaattatag ttaatatccc gaaaaagaaa
     2161 aagccccctc acacaaaggg ggcttgcaca atcgggtcag atagggaaat cagaactcag
     2221 aaaacgacgt ccggatggga aaaacgcagt ccggcaccgt cgtgataggt ggcatattcc
     2281 gtaactatcg ctccctcagg ggcatacatc cagtgccact gttcagcgcc gcccagctga
     2341 cccacttcac cgggcatcag ctttttctca cagcgtgcga tggcgcattc tttatgggac
     2401 ggcggaatcc tttcttccac cccgggcgtc ggctcgcctt caccgtagat atatacccat
     2461 ccgtgccggg gctgccagcc ttccattttc ggcgcagcct ctgtccgcac atggcggtgc
     2521 tcgggaatca tctgcccggg cagcaggtaa atatcgtgcc cgaaataatt atcttcctga
     2581 ttgttgaccc agaagatacc gcccatgccc acttcggcaa atttgccgag cccgaaatcg
     2641 acgacccaga aatctgcact ccgcagacgg ggcacaatgg gatagccgaa ttcttcaaac
     2701 attttgtagt aggcttcctt tgccttcgct tcatcgaagg tgccgtctgc cttgtaaaaa
     2761 tattcattgg gatgccgggt aatctcacgc ttctccgcag atgccatgac aattcctttc
     2821 ctggtcaatc cggcagcgac tgctgcagcc atgccggcca taaatttccg tctgcttaaa
     2881 atcatactcg cagcctttca acctgtattg cgctgccgtc agcagcattt tcctttcaga
     2941 tcattataca gggcaaaagc ctgtttcggc gattcgagcc ggtgcacaac agcacccacc
     3001 gcctgcgcca tggcgacagg gtcttccgca ccgaaaatat ttctgcccat gtccacaccg
     3061 agagcaccct gatccaccgc cttgtatgcc atggcaagcg cttcttcttc aggcagtttt
     3121 ttgccgcccg caatgaccac cggaataggg catccggcaa ccacagtctc aaagccgggc
     3181 tcgcaataat aagtcttgac aaaatgtgct cccacttcag caatcacccg gcaggcaagc
     3241 cccagatagc gcgcatcgcg taccatttcc ttgccgactg cggtaacgcc cagcgtgggc
     3301 atgccgaagg cgtttccctc attgatgaga taggcgagat tctccagcgt ttcccgctca
     3361 tactcggcac cgatgcaaac ctgtgtagtc acggcggcgg cgttgaacgc cagcgcatct
     3421 tccagagtca ccccgatcac ctcattgctc agttctttca gcacggtggc gcctgtgctg
     3481 cagcgcatca ccagcggctt gcgcacttcc gggctgatgc aggaacggaa ggcgccccgt
     3541 gtccccataa gacaatccac atgcgggatc agcggcggca ccacaagatc cagccgttcc
     3601 agcccggttg tcggccccat aatatagccg tggtcgaaag caagcataac ggttcgcccc
     3661 gacttcgggt taaaaatctg ggacaggcgg ttcttcattc cccagttcag gtgcgccgaa
     3721 cctttcagaa aaaagcccgg tgtttccatg ggaatatcca cgccgtactc ttttcttgca
     3781 tccgcatagt tatccgcatc aggcatgatc cgttattcct ttcataaatc ctttgaaaat
     3841 aagagcaatg gaagttgcac cgggatcggc gctgccgatg ctctgctcac cgatgttgcg
     3901 cgctctgccg aaacgcgcct gtaatgtttt tgtaaattct gcaccggcct ccgccgcttc
     3961 ggctgcacag tacagagcgg cggcaatgct ttcccctgcc cgtgcactgg cagacagtgc
     4021 ctccacggca ggaatgagcg catccaggag tgttttatcg ccgggctgcg ccttggtctg
     4081 ggcatgaaca cctgccagcc ccgcctcaaa ggcgcaggct aaggcttcgc cgtcaagaac
     4141 gtcgagtcct tcggcacttt ccgccatgga aatgaacatc atacccagca gcggaccgat
     4201 ggcgccgccg tccacaccca tgaccgccca gccgatctcc tgcagcagag tggcaatctt
     4261 ctggctttca tgggatttaa cggcatgcag cactttgccc atggcacgcg ccatggtggt
     4321 accatgatcg ccatcaccgc ccaccgaatc aagacgggac agatagtcct gcttttccag
     4381 aacactggcg gcagcacctt caaacatacg tgccagaccg tgataatcaa gggtgttcat
     4441 ttccggctac ttccgtgtcc aggcaggcgc atcacagggc gcatcccaga gcgctttcaa
     4501 ctcctcatcc agtttcccga tgcacatctg aaagccgccc atttcctgca cagtcagata
     4561 ttcaccgacg agcgaccgcg ccagtgtgat gcctttgtct gccagcagct gattgacccg
     4621 gcgcagcacc agatacagtt ccataagcgt tgtggcaccg gcaccgttca gcagcacaaa
     4681 aagttcatca cccgctttcg catcgatgtc atcgagaagg gcgttcaaca tgatttccgc
     4741 tgtttcatcg gcactcttca gagactgggt accgccgcct gcttcgccgt gctgacccat
     4801 accgaccacc atggtgcctt cgtctatggc tgcaatcact tcgcctgtgc tcggatgggt
     4861 ggcaccggat acggcaacag cgagagttgc catgcccgct tccatgcgcc gggcgatctc
     4921 cgcacattct tccaacgagc gtcccgcttc c
//
LOCUS       DAOPWB010000187         4943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_17117_length_4943_cov_5.722381, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000187 DAOPWB010000000
VERSION     DAOPWB010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17117_length_4943_cov_5.722381"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..599)
                     /gene="dtd"
                     /locus_tag="PKN92_12365"
     CDS             complement(147..599)
                     /gene="dtd"
                     /locus_tag="PKN92_12365"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNZ49628.1"
                     /translation="MRAVIQRVSEASVSVEGRCVGRIASGLMVLLGVDISDDAKDAEL
                     MAEKIAFLRVFSDPDGKMNVAVQDSGGKVLLISQFTLLGDCRKGRRPSFITAARPEQA
                     VPLLQKVRELLLNKYEIPVEEGVFGAHMQVSLINDGPVTLLIDTKKYF"
     gene            complement(621..1337)
                     /gene="aat"
                     /locus_tag="PKN92_12370"
     CDS             complement(621..1337)
                     /gene="aat"
                     /locus_tag="PKN92_12370"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNZ49629.1"
                     /translation="MPIYFLDDDSLTFPPATHTHNGLLAVGGDLSPSRLLAAYQKGIF
                     PWYSDGEPILWWSPDVRMVLFPENFHLSRSLRRFCRATPFSFRMDTAFRQTISYCAGA
                     ERSGENGTWIHPEMIEAYCTLHEMGFAHSVECYRNGSLVGGLYGINLGSAFFGESMFS
                     LEKNGSKFALAVLSAQCRLWGFKFIDCQMYTPHLASLGAVEMTKAKFLKILKEALKEK
                     NRKGKWIADEQIILQEILNP"
     gene            complement(1355..2473)
                     /gene="hemW"
                     /locus_tag="PKN92_12375"
     CDS             complement(1355..2473)
                     /gene="hemW"
                     /locus_tag="PKN92_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNZ49630.1"
                     /translation="MASSPPLGVYIHIPYCRRICRYCDFVHEPCPASVPHDFIDSLVH
                     EIMTSSSGGVVDSVFLGGGTPSLLTAKDLQFIVGALHKKYDIQSPEFTLEANPDDVTT
                     EKLNYWQEYGINRISLGVQSFSDDTLRFLGRRHTAKTALDAAAKIAETFENWNLDLIY
                     GAPPLSSWKKTLAAALSFDPPHIAAYALGYVPGTELGDCGLKPYADLTVLRQYRRAEE
                     MLSAYNHYEISNFAKKGKESRHNLKYWRNEEHDAYGPGAYSLRGNRRQCNIPFVESYI
                     VDPLYKAEDQILSPLQIKLETMIQHMRLKEGITEAYYYNRFQENIADLFGFILKKLVA
                     DGLVHHKNNTYFPTRKGFYFNDTLGLQLVSSFENIIDV"
     gene            complement(2494..3888)
                     /gene="lepB"
                     /locus_tag="PKN92_12380"
     CDS             complement(2494..3888)
                     /gene="lepB"
                     /locus_tag="PKN92_12380"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNZ49631.1"
                     /translation="MSQKNKNRDISADEQISPLIPAYAAYIAAFLFVAGIVSMYFLGR
                     NGQDSFTWKNYLAIALTVCSAVLWFISGMDRTQVLDWFRSGFIALFLALTIRWAIAEP
                     YRIPSGSMETTLHGDPRFGRGDRVFVNKWIYGLRYPFLNKRIWQGKMPERWDIVVFKA
                     VEPDAVHKTLVKRIVGMPGEEVQIRSGRVYINGTPLTLPESIGQEIYYTSPLDGPYGV
                     QSLPQYSQIPDGHYLLLGDNSAHSRDGRYFGWVPNENMVGRVSSIWWPPPRWRDFTGF
                     SQQLWWRGGITVFLLLAVLRLLAGRVCSAKNANGPRGQRCVISFLSYGLLVPFLLKRI
                     ITWRRPASGELVLFKVKDPLTKSDFLFLGRIAAVAGERVTFDNGRLCINGSLPESGYF
                     VNKSFETETGNIKYGRIKSKEFSTVPEHAYFLLSDDSAPGACEHDSRTLGWISEDAIV
                     GKAVFCCCGVKQAD"
     gene            complement(3993..4379)
                     /locus_tag="PKN92_12385"
     CDS             complement(3993..4379)
                     /locus_tag="PKN92_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNZ49632.1"
                     /translation="MALSKKDLEKFKKLLLAEKERLIASISAAEHISRTEAEGDHGQE
                     ISDYAEIGTDNFFLETTLSIASNESVRLREVLDALQRIEDNKYGICEGIGKPIPKKRL
                     EAIPYARYCIEYQEQIENESATHRYR"
     gene            complement(4596..>4943)
                     /locus_tag="PKN92_12390"
     CDS             complement(4596..>4943)
                     /locus_tag="PKN92_12390"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNZ49633.1"
                     /translation="SLKNLITRAVGIRDTVDVDLFALELQPHDTLLLCSDGLTNMVND
                     ENLEKELAQEDLTAMSESLLEKALEAGGTDNITMILVRIGEVLKESNYQQGGRIISFH
                     KKSALRRLFSFFS"
BASE COUNT         1385 a         1203 c         1097 g         1258 t
ORIGIN      
        1 atgaaacaac gaaactctca taacgactgg accaaagaat gggggcttga cacccacctg
       61 tgatcatgcc ttctatccca gtgcccttca accactttcg aatttcgcac ccgcctgagt
      121 gcatgcatgc gttttcttgc agctggctaa aagtattttt tggtatcaat caagagggta
      181 accggaccgt cgtttatcag agacacctgc atgtgcgccc cgaaaacgcc ttcctcgaca
      241 ggtatttcat atttattcag cagcagttcc ctaacttttt gcagaagcgg aacagcctgt
      301 tcgggacgtg ccgctgtaat gaaagaaggg cgccttcctt ttctgcaatc cccgagcagg
      361 gtaaattgag agatcagcag tacttttccg cctgaatcct gcaccgctac attcattttt
      421 ccatcaggat cgctaaaaac acgtaggaaa gcaatttttt cagccatcag ttccgcgtct
      481 tttgcatcat cacttatgtc cacaccaagc agaaccatga gaccggatgc tatccggccc
      541 acacatcttc cctccacgga aacagacgct tccgacaccc gctgaatcac ggcgcgcata
      601 aagtcttcct tacagtcttt ttatggattg agaatctcct gcaagattat ttgctcgtca
      661 gcaatccact ttccttttct attcttttct tttaaggctt ccttaagaat cttaagaaat
      721 tttgctttag tcatttcaac tgctcccaga gaagccaagt ggggcgtata catctggcag
      781 tcgataaact tgaatcccca cagcctgcat tgagcgctca aaacggcaag agcaaatttt
      841 gatccattct tttcaagtga aaacatggat tcgccaaaaa aagcactgcc tagatttata
      901 ccgtaaagac caccgaccag actcccattc cgatagcact ccacagagtg ggcaaagccc
      961 atttcatgaa gggtgcagta ggcttcgatc atttccggat gaatccaggt tccattttcg
     1021 cccgaacgct ctgcaccggc acaataggaa attgtctgcc tgaatgcagt atccattcta
     1081 aaagaaaagg gtgtggcacg gcagaaacgg cgcagacttc gggataaatg aaaattctca
     1141 gggaaaagca ccatccgcac atcaggactc caccaaagga taggctcacc gtctgagtac
     1201 caggggaaaa ttcccttttg atatgcggca agaagtctgc tcggcgataa atctccgcct
     1261 acggcaagca acccgttgtg cgtgtgtgtg gccggcggaa aggtgagaga gtcatcatcc
     1321 agaaagtata taggcatgct ctgcctccgc ccatttatac gtcaataatg ttttcaaagc
     1381 tcgacaccag ttgcagaccg agggtgtcgt taaaataaaa gccttttctt gtcgggaaat
     1441 aggtattgtt tttatgatgc acgagaccgt ccgcaaccag cttttttaaa atgaaaccaa
     1501 acagatctgc aatattttcc tgaaaacggt tgtagtaata cgcttcagta attccttctt
     1561 ttaaccgcat atgctgaatc atcgtctcca gtttaatttg caggggagaa agaatttgat
     1621 cttcagcctt atataaagga tccacaatat aactttctac aaaaggaata ttgcactgcc
     1681 gacgattgcc tcttaacgaa taagcgcccg gcccataggc atcgtgttcc tcattgcgcc
     1741 agtactttag attatgacgg ctctctttgc cttttttagc aaagtttgat atttcataat
     1801 gattgtaggc agaaagcatt tcttccgcac gtcggtattg ccgaaggaca gtgagatctg
     1861 catagggctt caatccgcag tcgcctagtt ctgtccctgg cacataccct aatgcgtatg
     1921 ctgcaatgtg aggaggatca aaagaaagag ccgctgcaag tgtttttttc caggaactta
     1981 gaggaggcgc tccgtaaatc agatcaagat tccaattctc aaaagtctct gcaatttttg
     2041 ctgcagcgtc aagcgctgtt tttgctgtat gtcgcctgcc cagaaaacgc aaggtgtcat
     2101 cggaaaaact ttgcacgccc aggctgatcc tgttgatacc atactcctgc caatagttaa
     2161 gtttctctgt tgtgacatca tcaggattag cttccaatgt gaattcaggg ctttgtatgt
     2221 cgtatttttt atgaagggca cccacaataa attgcaggtc tttcgccgtc agaagcgatg
     2281 gagttcctcc gcctaaaaaa acactgtcca caacaccgcc tgaggaagaa gtcataattt
     2341 catggacaag agagtcaata aaatcgtgag gcaccgaagc agggcatggt tcatgaacga
     2401 aatcacaata ccggcaaatg cgtcggcaat aaggaatatg aatgtatact ccgagaggcg
     2461 gggaagatgc catgacaaac ccctgtggct ggattaatcc gcctgcttaa cgccgcagca
     2521 gcagaagact gctttaccaa caatcgcatc ttcggaaatc caacccagtg tccggctgtc
     2581 atgctcgcag gcacccggcg cactgtcatc gctaagcaga aaatacgcat gttccgggac
     2641 agtggaaaac tctttggatt taattctgcc gtattttatg tttcctgttt ccgtttcgaa
     2701 agatttgttt acaaaatatc ctgattctgg caaagaacca tttatacaca aacgcccatt
     2761 gtcaaaagta acacgctcgc ccgcaacagc ggcaatacgt cctagaaaca agaaatcact
     2821 ttttgtaaga ggatctttta ctttaaacag tacaagttct ccggaagcag ggcggcgcca
     2881 cgtaataatc cttttgagta aaaacggaac aagcagcccg taactcaaaa aagaaataac
     2941 acagcgctgt ccccgggggc cattggcgtt ttttgcagaa caaactcgcc cggcaagaag
     3001 gcgcaagaca gccagtaaca aaaacacagt aatgccgcca cgccaccaaa gctgctgaga
     3061 aaagcccgta aaatcacgcc aacggggcgg tggccaccag atagacgata cgcgccccac
     3121 catattttca ttgggtaccc atccgaaata acgaccgtca cggctgtgtg cgctgttgtc
     3181 cccaagaaga agatagtgcc cgtcaggaat ttgtgagtat tgtggtaagg actgaacgcc
     3241 atagggaccg tccagaggcg atgtataata aatctcctgc ccgatacttt caggaagggt
     3301 caaaggcgtc ccgttgatgt atacacgccc actgcgaatt tgcacttctt cgccgggcat
     3361 cccgacaata cgtttgacca gcgttttatg cacagcatca ggctcgactg ccttaaaaac
     3421 cacaatgtcc cagcgctccg gcattttacc ctgccagatt cgcttgttta aaaagggata
     3481 ccgcagtcca taaatccatt tattcacaaa gacgcggtca ccacgaccga agcgaggatc
     3541 gccatgcagt gttgtttcca tggagcccga aggaattctg tacggttctg ctattgccca
     3601 gcggatagtt aaagccagga acaaggcaat gaagccgctg cgaaaccagt caagaacctg
     3661 tgtccgatcc ataccgctta taaaccacag cacagcactg cagacggtaa gggcgatggc
     3721 cagatagttt ttccaggtaa aagagtcctg tccgttgcgt ccgagaaaat acatggacac
     3781 tatgcccgcc acaaacaaaa aagcggcaat ataggctgca taggcaggga tcagagggga
     3841 aatctgctca tcggcagaaa tgtctctgtt tttatttttt tgtgacataa gaaatcgtat
     3901 tcttacaggc aacagaaatg ctgttaaacc agtcgcatcg gttaatttct gccggagtgg
     3961 ctgttatgaa aaaagaaggc atcggatagt gactaacggt accgatgcgt agcactctcg
     4021 ttttcgatct gctcctggta ttcaatgcaa taccgggcgt aagggatcgc ttccagccgt
     4081 tttttcggga taggttttcc tatgccttca caaattccgt atttgttgtc ttctattcgc
     4141 tgcaatgcgt ccagcacttc gcgcaagcgt accgattcat tgctggcgat ggacagtgtc
     4201 gtttccagaa aaaaattatc agtgccaatt tccgcataat cagatatttc ctgaccgtgg
     4261 tcgccttcag cctctgttcg ggaaatatgc tcggcagcgg aaatgctggc aattaaacgt
     4321 tctttctcgg caagaagcaa ctttttaaat ttttcaagat cttttttgct gagcgccata
     4381 gtaatatcct cctgtctgat ccgggattat gcggccgcca cctaatgtcg gtttaaaaac
     4441 gaccgttcaa atgttatatg gcttattagg aaagtaagtt tagcagatgg aggaagcctt
     4501 ttcaacaaaa ggaaattaaa aagcagcgta cacacctcgg gagaaagggg gaaaaagaaa
     4561 gggcaggcgg gcaaaaaatt caccgactca atggcttaag aaaagaagga gaaaagccta
     4621 cgcagagcac tttttttatg aaaggaaata atgcggccgc cttgctgata attggattct
     4681 tttaaaactt caccgatacg aacaaggatc atggttatat tgtctgtacc gccagcttct
     4741 aaggcttttt cgagcaggct ttctgacatt gccgtcaggt cttcttgtgc aagctctttt
     4801 tcaagatttt cgtcgttcac catattggtc agaccgtctg aacaaagaag aagtgtatcg
     4861 tggggctgca attccaatgc aaagagatcc acatcaacgg tgtcgcgaat acctacggcg
     4921 cgggtaatca aattttttaa cga
//
LOCUS       DAOPWB010000188         4882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_17414_length_4882_cov_5.484152, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000188 DAOPWB010000000
VERSION     DAOPWB010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17414_length_4882_cov_5.484152"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PKN92_12395"
     CDS             <1..1157
                     /locus_tag="PKN92_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNZ49634.1"
                     /translation="RTWQQAGRAGRRSTTALIVLVASSAPLDQYIINHPDYFFDQTPE
                     NATIDANNLFVLSSHLKCAAFELPFTEGDTFGLDAASTAELLDFLAQHRVLRKAKNRW
                     HWSAESYPASDISLRTAAPGNVVILDTTDNGRVIGEVDYFNAPVEVYEHAIYLHETRQ
                     YTIERLDLEDRKAYARPVEVDYYTDAEQKVDLRVIERFEESGLLEANVCRGEVSVTWL
                     PTIFKKIKFGTHENVGWGEIHLPEQTMHTTGFWIEFPEEIASMLQLGQEALGEALHSL
                     ANALRQVAPVSILCDPQDILATARLKAPESQRPSVFLYERYPGGVGYSEKLFHHQAAL
                     IRAVIALLSDCGCREGCPSCVGPTLEAGLHGKRGALALARFCLKESGTAE"
     gene            complement(1161..2222)
                     /locus_tag="PKN92_12400"
     CDS             complement(1161..2222)
                     /locus_tag="PKN92_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNZ49635.1"
                     /translation="MTISVDPVRNLPSSTLRPWIAATAVLLLLLPGCGGTPKQIRLHG
                     EALGTFYAITVADPPANLSESDVRKLADNTLDRINVLMSTYREDSELSRFNRHEDTSP
                     FLLSPETYAVFSIAGEISEKSGGAFDITVGPLVNAWGFGPDPLRDPPTEEQIRELLSH
                     QGYEKLRFLENYYVAKEDPKIYCDLSAVAKGYAVDAIAEAFEAAGLSRYMIEVGGELR
                     VAGKNDKGQRWGLGIEKPLVGRRELETVVFLEKESLATSGDYRNSYELGGKRISHTID
                     PATGHPVQHALASVSVIHPSCAYADGYATALMVLGPEKGLALAEKEKLAVMMLVHDEK
                     EGGFQRITTPAFDAYLTGE"
     gene            complement(2206..3426)
                     /gene="nqrF"
                     /locus_tag="PKN92_12405"
     CDS             complement(2206..3426)
                     /gene="nqrF"
                     /locus_tag="PKN92_12405"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005977006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNZ49636.1"
                     /translation="MVTIVMGVVMFCFVVLSLVGVLLVAKWKLVSSGDVKILINEDES
                     KALVTPSGGTLLATLAQHGIFVPSACGGKGTCGVCKVAVQEGGGSLLPTEEAHISKRE
                     AREDIRLSCQVKVKADLKITIPPEIFDVRKWECTVRSNHNVATFIKELVLVLPEGESV
                     PFRAGGYIQIECPPHTAKYSDFLVEEQYRDAWDRSNLWRFESVVDETVTRAYSMANYP
                     EEHGIIMLNVRITPPPPGTDFPPGIMSSYIFNLKPGDKVTISGPYGEFFAKDTEREMC
                     FIGGGAGMAPMRSHIFDQFRRLKTKRKATFWYGARSLREMFYVEDFDSIAAENENFTW
                     YTALSEPQPEDNWNGLTGFIHQVLFDEYLSSHPEPEEIEYYLCGPPMMLDACRKMLAS
                     LGVPDEMIMYDDFG"
     gene            complement(3458..4072)
                     /gene="nqrE"
                     /locus_tag="PKN92_12410"
     CDS             complement(3458..4072)
                     /gene="nqrE"
                     /locus_tag="PKN92_12410"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009833701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNZ49637.1"
                     /translation="MEHYLSLFVRAVFIENMALAFFLGMCSFLACSKKVSNAMGLGVA
                     VVAVMAITTPICNLVYNWLMAPGAMAWIHPSLAKVDISFLYFLSFIGVIAAIVQILEM
                     LLDRFVPWLYAALGIFLPLIAVNCAILGAALFMVERRYTFGESFVYGTGAGLGWALAI
                     TCMAGIRERMKYSNVPPALRGLGIAFMLTGLMALGFMSFSGIQL"
     gene            complement(4074..4700)
                     /locus_tag="PKN92_12415"
     CDS             complement(4074..4700)
                     /locus_tag="PKN92_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNZ49638.1"
                     /translation="MLNKKQREALLDPIFNNNPIALQILGVCSALAVTTNLATALVMG
                     LAVTFVTGGSSAAVAAIRNSIPPSIRIIVQLSIIASFVILTDQILKAFLFDISKQLSV
                     FVGLIITNCIVMGRAEAYAMQNGPWLSFLDGVGNSLGYTLILLCVGTVRELFGSGSLF
                     GFVLLQKISDGGWYEPNGLMLLSPSAFFLIGIFVWLLRLWKPEQQELE"
     gene            complement(4703..>4882)
                     /locus_tag="PKN92_12420"
     CDS             complement(4703..>4882)
                     /locus_tag="PKN92_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNZ49639.1"
                     /translation="PAITVVKGSAGSVEEAPHEVDGLSGATITSRSVMEMLRFWLGDD
                     GFGPFLKQYRESGQA"
BASE COUNT         1172 a         1380 c         1320 g         1010 t
ORIGIN      
        1 cccgtacctg gcagcaggcg ggccgggcag gcaggcgcag caccacggca ctcattgtgc
       61 tggttgcgtc cagtgcgccc ctcgaccagt atatcatcaa ccaccctgac tacttttttg
      121 atcagacccc tgaaaatgcc accatcgatg cgaacaatct ttttgtattg tcaagccatc
      181 tgaaatgtgc cgctttcgag ctgcccttca cagaagggga taccttcgga ctggatgccg
      241 ccagtaccgc ggagctcctg gattttctgg cacagcaccg ggttttgcgc aaagcaaaaa
      301 accgctggca ctggagcgcg gaaagttatc ctgcctcgga tatcagcctg cgtacggctg
      361 caccgggcaa tgtggtgatt ctggacacga ccgacaacgg cagagtcatc ggtgaagtgg
      421 attatttcaa tgcgccggtc gaagtatatg agcatgccat ctacctgcac gagacccgcc
      481 agtataccat tgagcgtctg gatctcgaag accgtaaggc ctatgcacgt cccgtggaag
      541 tggactacta taccgatgcg gagcagaagg tggacctgcg tgtcattgag cgttttgagg
      601 aaagcggact gctggaagcc aatgtctgcc gtggtgaagt cagtgtgacc tggctgccca
      661 ccatcttcaa aaagattaaa ttcggcaccc atgaaaatgt cggctggggc gagatccacc
      721 tgcccgagca gaccatgcac accaccggct tctggattga atttcccgaa gaaattgcgt
      781 caatgctgca gctcggacag gaggcgctgg gcgaagcgct ccattccctc gccaatgcac
      841 tgcgccaggt ggcaccggtc tccattctct gcgatccgca ggacatactg gcgacagcac
      901 ggctcaaagc gccggaatcg cagcggcctt ccgtttttct ctacgaacgg tatccgggcg
      961 gggtgggcta cagcgaaaaa ctgtttcatc atcaggcggc actgatccgg gctgtcatcg
     1021 cgctgctttc agattgcggc tgccgggaag gctgccccag ctgcgtgggc ccgacgctgg
     1081 aagcggggct tcacggaaag cggggcgctc tggcgctggc ccgattctgc ctcaaagaaa
     1141 gcggtacagc agaataacct ttattccccc gtcaggtaag catcgaatgc cggggtggta
     1201 attcgctgaa agccgccttc cttctcatca tgcacgagca tcattaccgc cagcttttcc
     1261 ttttccgcca gagccagtcc cttttcggga ccgagtacca tcagtgctgt ggcatagcca
     1321 tcggcatagg cgcatgaggg atgtatcacc gacacggaag ccagggcatg ctgcaccgga
     1381 tgacccgttg ccggatcaat ggtatgggat attcgctttc cgcccagttc atagctgttc
     1441 ctgtaatccc cggaagtggc aaggctctct ttttcgagaa agacaaccgt ttccagctct
     1501 cttctgccga ccagcggttt ttcaataccc agaccccagc gctgtccttt gtcgtttttt
     1561 cccgccaccc gcagctcacc gcccacttca atcatataac gggataatcc tgccgcttca
     1621 aaagcctcgg ctatggcatc gaccgcatag cccttggcaa cagcagaaag atcgcaataa
     1681 attttcggat cttctttggc gacatagtag ttttccagga atcgcagctt ttcatagccc
     1741 tgatggctca gcagctcccg gatctgttct tccgtgggcg gatcacgcag cggatcaggc
     1801 ccgaagcccc aggcgttcac aagaggaccc actgtgatat caaaggcacc gccgcttttt
     1861 tcggatatct ctccggcaat ggaaaaaacg gcataggtct ccgggcttaa aagaaagggg
     1921 ctcgtatcct cgtggcggtt gaaacggctc agctcggaat cttcacggta ggtggacatg
     1981 agtacattga tgcgatccaa tgtattgtct gccagtttgc ggacatccga ttcggagaga
     2041 ttcgccggag gatccgccac ggtgatggcg tagaaggtac ccagcgcctc gccgtgcagg
     2101 cggatctgct tcggtgttcc accgcaaccg gggagcagca ataaaagaac agcggtcgca
     2161 gcaatccacg ggcgtaaagt gctggagggc agattccgca ccggatcaac cgaaatcgtc
     2221 atacatgatc atttcatcgg gcacgcccag gctggcaagc atttttctgc aggcatccag
     2281 catcatcggc ggtccgcaaa gatagtattc gatctcttca ggctcggggt gggaggaaag
     2341 gtattcatca aaaagaacct gatgaataaa cccggtcaga ccgttccagt tgtcttcggg
     2401 ctgtggctcg gacagcgcgg tataccaggt gaaattttca ttttcagccg caatggaatc
     2461 aaagtcttcc acatagaaca tttcacggag actgcgcgca ccgtaccaga aggttgcttt
     2521 gcgtttggtc ttgagccggc ggaactgatc gaaaatatgc gagcgcatgg gcgccatgcc
     2581 cgcaccgccg ccgatgaagc acatctcccg ttccgtatct ttggcgaaga attcgccata
     2641 gggaccggaa atcgtcactt tatcgcccgg cttcaggttg aaaatatagg aagacatgat
     2701 gcccggaggg aagtctgtgc ccggcggcgg cggcgtaatg cggacattca gcatgatgat
     2761 cccgtgctct tccggatagt tcgccatgga ataggcacgg gtcactgttt cgtctacgac
     2821 agattcaaag cgccacagat tggagcggtc ccaggcgtcg cggtactgct cttccaccag
     2881 gaaatcactg tatttcgcgg tatggggcgg gcattcgatc tggatatagc cgccggcacg
     2941 gaagggcacc gattcgcctt ccggcagcac cagcaccagt tccttgatga aggtggcgac
     3001 gttgtgattg gaacggacgg tgcattccca tttcctgaca tcgaagatct ccggaggtat
     3061 ggtaattttg agatccgctt tcactttgac ctggcaggaa aggcggatgt cttcccgtgc
     3121 ctcgcgtttg ctgatatgcg cctcttcggt aggaagcagc gagccgcctc cttcctgaac
     3181 ggcgactttg cagacaccgc aggttccttt tccgccgcag gcggagggca caaagatccc
     3241 gtgctgcgcc agtgttgcga ggagcgtacc gcccgacggc gtgaccagcg ctttcgattc
     3301 gtcttcatta atcaggattt ttacatcgcc tgatgagacg agcttccact ttgccaccag
     3361 cagtacgccg accagcgaca gcacgacaaa gcagaacatg acaacaccca ttacaatggt
     3421 aaccatgccg cccatgcgga agatctcctt aaaaagttta gagctggatg cccgagaagg
     3481 acataaatcc caaagccata agccctgtca gcatgaacgc aatacccagt ccacgcagtg
     3541 caggcggcac gttgctgtat ttcatgcgtt cacgaattcc tgccatgcag gtaatcgcca
     3601 gtgcccagcc aagcccggcg ccggtgccgt atacaaaact ttcaccgaaa gtgtagcgac
     3661 gctccaccat gaaaagagca gcacccagaa ttgcgcagtt caccgcaatc agcggaagaa
     3721 aaataccgag ggcggcgtaa agccagggca caaaccggtc aagcagcatc tccagaatct
     3781 gcacaatggc ggcaatgacg ccgatgaaac tcaggaaata aagaaaggaa atatccactt
     3841 ttgcaagaga aggatgaatc cacgccatgg cgcccggtgc catcagccag ttgtacacca
     3901 gattgcagat gggtgtggtg attgccatga ccgccaccac agccaccccc agacccatgg
     3961 cattggatac cttcttggag caggcgagaa aagaacacat gcccaggaaa aaggcaagtg
     4021 ccatgttctc aataaagacg gcccggacaa aaagacttaa ataatgttcc atatcactcg
     4081 agctcctgct gctcgggctt ccacaggcga agcagccaca caaaaatacc gataaggaag
     4141 aatgcactgg gtgaaagcag catcagaccg ttgggttcat accagccgcc gtcggaaatc
     4201 ttctgcagca gcacaaagcc gaagagactg cccgagccga aaagttcgcg caccgtgccc
     4261 acacagagga gaatcagggt gtagcccagg gagttgccga cgccgtccag aaaactcagc
     4321 caggggccgt tctgcatggc atacgcttca gccctgccca tgacaatgca gttggtgatg
     4381 atcagaccca caaaaacgga cagctgcttg ctgatgtcga aaagaaatgc tttcagaatc
     4441 tggtcggtca gaatcacaaa agaggcaata atcgacagtt gcacgataat ccggatgctg
     4501 ggagggatgc tgtttctgat ggccgccacg gcagcagaac tgccgcccgt gacaaaagtc
     4561 accgcaagac ccatgaccag cgccgtggca agattcgtcg tcacagccag agcggaacag
     4621 acgcccagta tctgcagtgc aatagggttg ttattgaaaa tgggatccag cagtgcttca
     4681 cgctgtttct tgtttaacat gctcatgcct gtccgctctc cctgtattgt ttaaggaaag
     4741 gaccaaatcc atcatcgccc agccagaacc ggagcatttc catgacagaa cggctggtaa
     4801 tggtggcacc gctgaggcca tccacttcgt ggggcgcctc ttccaccgat ccggcagagc
     4861 ctttcaccac cgtaatggcg gg
//
LOCUS       DAOPWB010000189         4876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_17451_length_4876_cov_4.634101, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000189 DAOPWB010000000
VERSION     DAOPWB010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17451_length_4876_cov_4.634101"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..617)
                     /gene="pyrR"
                     /locus_tag="PKN92_12425"
     CDS             complement(39..617)
                     /gene="pyrR"
                     /locus_tag="PKN92_12425"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNZ49640.1"
                     /translation="MSRETDVETTILMDADAMAATLNSMALAVVNGNPDLGSLALLGI
                     LRRGEPLAIRIAGLIEKATGIAPPVGALSTTLYRDDLHAGSAVDVTSRSGETYFDFSL
                     EGRTVLLVDDVLSAGRTIRAAMGEIMDYGRPRRIQLACLVDRGGRELPIQADYLGYSV
                     ETTEEEWVSVRLREVDGEDLVLVHKRSSERNL"
     gene            complement(760..1371)
                     /locus_tag="PKN92_12430"
     CDS             complement(760..1371)
                     /locus_tag="PKN92_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNZ49641.1"
                     /translation="MFSIIVTGTFLGFSAGLSPGPLMTIVIAKTLQHGTREGIKASFA
                     PLITDAPIIVATLLFLKPVAHLDPIMGMLALGGAALLFYLGHENWTAPLPGQIQNVNA
                     GSSIRQAALVNIFSPNPWLFWLTVGAPILHEEWAKHPAYPFLFLIFFYALLLGSKTVI
                     ACSLGIYGHFLKDKQRILVIRFLSCILFIYAFILLWKAWGLLF"
     gene            complement(1525..1698)
                     /gene="rpmG"
                     /locus_tag="PKN92_12435"
     CDS             complement(1525..1698)
                     /gene="rpmG"
                     /locus_tag="PKN92_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNZ49642.1"
                     /translation="MAAKAKGNRETIILECTEAPGTSRYNTEKNKRNNPERMELKKYN
                     AVLRKHTVHREKK"
     gene            complement(1756..2226)
                     /gene="hisI"
                     /locus_tag="PKN92_12440"
     CDS             complement(1756..2226)
                     /gene="hisI"
                     /locus_tag="PKN92_12440"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNZ49643.1"
                     /translation="MVDFAERKSVEQIEEGTELAPKFDAQGLIPVVTTDYESGELLMH
                     AYMNEEALKKTIELGEAVYWSRSRKVLWHKGATSGLVQKVRELRIDDDQDTVWLRVDV
                     QGGASCHVGYRSCFYRTVPVGADATERITLSFTETEKVFDPKKVYGDAPNPTQI"
     gene            complement(2529..4250)
                     /gene="ptsP"
                     /locus_tag="PKN92_12445"
     CDS             complement(2529..4250)
                     /gene="ptsP"
                     /locus_tag="PKN92_12445"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNZ49644.1"
                     /translation="MEIYLQGMGVSPGIAIAPALFYSRASFEVPRRPITDVQAELMRL
                     DEAVAKVRRELTSIYKKTVDAVGKAHAEIFRVHLMILDDVALRDDLAAQMEQERLNVE
                     FILSSIASKYTEMMNNIDDQRFRERTADLLDVVDRLQRHLLKSDGPLLRHISEPSILV
                     AQDLAPSDTASLDLDKALGLLVETGSPTSHSAILARALEIPAVVGISISQKSLSPGSL
                     MILDGTSGVVIINPSQETVEKYRTEQQRLQSIQSHLLESLLSGPACTKDGTAVTLESN
                     IELPLEIKHSLKMNVQGVGLYRTEYLFFNRDTLPTEEEQYQAYLHVASELFPAPVTLR
                     TMDIGGDKFVKYLQNYKDSNPQLGLRAIRLSLAYPDIFKVQLRAMLRASAQGNIRIMF
                     PMISSLEELRHAKAILQEARQELSSEGVAYNPDLKVGSMIEVPSAAVQADQIAEECDF
                     FSLGTNDLIQYLLAVDRVNEKIAHLYEPAHPAVIRMIANVSSAAAAHNIPCSICGEMA
                     GDPLYTELLLGLGVSSFSMASVSIPLIRARIAALNREDAKHLARDILQKKTAEEIKTI
                     LRSRLEG"
     gene            complement(4271..4552)
                     /locus_tag="PKN92_12450"
     CDS             complement(4271..4552)
                     /locus_tag="PKN92_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNZ49645.1"
                     /translation="MSELLEDVIVINPLGIHARPAALLAQSIAHFQSDVYLLFNGSRV
                     NAKSIMGLLTLGAARGSRISVSCIGEDADEAMARVKAIFESGFNEIKSD"
     gene            complement(4605..>4876)
                     /locus_tag="PKN92_12455"
     CDS             complement(4605..>4876)
                     /locus_tag="PKN92_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49646.1"
                     /translation="KMRPLRLLAVKYAIISKIRERVKHDILGCVKKDKIIRPFWYRQK
                     TVIPQPNPTEALVSVFRAAGSAGALQASLIAGRPFLNIQVQSTES"
BASE COUNT         1241 a         1216 c         1214 g         1205 t
ORIGIN      
        1 cagagcgtgg gagggggttg ttccatacaa gaggttcctt acaaattccg ttcgctgctg
       61 cgcttgtgca ccagtaccag atcttctccg tccacttcgc gcagccggac agaaacccat
      121 tcttcttcgg tggtttccac actgtatccc agatagtccg cttgaatagg aagttcccgg
      181 ccgccgcgat caacgaggca ggcaagctga attcttctgg gacgtccata atccatgatt
      241 tcgcccatgg cggcacggat agtccgtccg gcagaaagga cgtcgtcaac cagcagtact
      301 gtgcggcctt caagagaaaa atcaaaatag gtttcgccgc ttctggaagt aacatccact
      361 gcagagcctg catgcagatc atcacggtac agagttgtgg agagggcacc gacgggaggg
      421 gcgattccag tggctttttc aattaatccg gcgatgcgta tggcgagagg ttccccccgg
      481 cgcaggatac cgagcagtgc cagagatccc aaatcaggat tgccatttac gactgcaaga
      541 gccatgctgt tcaaagttgc cgccattgca tcagcatcca tgaggatggt ggtttcaaca
      601 tctgtttctc gactcataca gggcttcctt catcttgaat gtacagacgt gcaagcagaa
      661 gatacacttc aaaaggcggg aaatacagaa gtgccacttg cgcctgtcgc ctgtgctcat
      721 gcggtaacac tatagcaaaa acagaacgga acctgctact taaaaaagaa gaccccaggc
      781 tttccatagg agaataaaag cataaataaa aaggatgcag gaaaggaagc gtataacaag
      841 aatccgctgt ttatccttga gaaagtgacc gtagataccc aatgaacagg cgatgaccgt
      901 tttggaaccc agcagaagcg cgtaaaagaa aattaggaaa agaaatggat acgccggatg
      961 ttttgcccac tcttcatgca gtatgggcgc gcctacagtc aaccagaaaa gccaggggtt
     1021 ggggctgaaa atgtttacaa gagccgcctg ccgtatcgaa gatccggcgt tcacattttg
     1081 gatctggcca ggcaagggcg cggtccagtt ttcatgacca agataaaaaa gaagcgcggc
     1141 tccccccaaa gccagcatcc ccattatcgg atccagatgt gctactggct ttaaaaataa
     1201 aagggtcgcg acaatgatgg gtgcgtcggt tatgagggga gcaaacgacg cttttatgcc
     1261 ctctcgcgtg ccgtgctgga gggtttttgc gataacaatc gtcataagcg gtcccggtga
     1321 taagccggca gaaaacccca gaaaagtacc tgtgacgatg atggaaaaca tggaactgct
     1381 tttttttcgg tcctaaaacg agtgaaaaac acccgggccc cgccgtcaat agatgcgttg
     1441 acatgggatg cccgggtgaa tacagaaata ccgcttcgga atagaaatgg tcagtcccgg
     1501 ctgtttgcag ccggaatggt gcgactattt cttttcccga tgcacagtgt gtttacgcaa
     1561 cacagcgtta tactttttca gttccatgcg ctccggatta ttgcgtttgt ttttctccgt
     1621 attgtatctg gaggtgcccg gtgcttctgt gcattcaaga ataatggtct cgcgatttcc
     1681 tttagctttg gctgccatgt ctgtaatcct tattttgtct caaaaagaag tgttttaaag
     1741 attgtcgata ctctattaaa tctgcgtggg gttgggagca tccccataga cttttttcgg
     1801 atcaaaaact ttttctgttt cagtgaagct gagcgtgatg cgctcagttg catcggcgcc
     1861 gaccgggacc gtgcggtaaa aacaggaacg gtagccaaca tgacaactgg caccgccttg
     1921 cacatctacg cgcagccaca ctgtgtcctg atcatcatca atacgaagct cgcgcacttt
     1981 ttggacgaga ccgctggttg cccctttgtg ccagagcact ttgcggcttc tactccaata
     2041 tacggcttct ccaagttcaa tagtcttttt aagcgcttct tcgttcatat aggcgtgcat
     2101 gagcaattcg ccactctcat aatcggtagt aacaacggga atcagaccct gagcatcgaa
     2161 tttcggagcc agttctgtcc cttcttcaat ctgttcaacg cttttgcgtt cggcaaaatc
     2221 aaccacgtaa ttactcctga aaacaagata atattctaca gcaggccggg ataaaaaggt
     2281 gttcttgtgc tttggaatcg cattaattcc acacagagac agaatagcct gatgttattc
     2341 tgaaaatgag gaaaggagac agctccgctt taaaaagaca caccgaaccc cggcttgttt
     2401 ttaatagtat acctgaaaag ggtggctcat tgcatcctga ccgacacgaa gggtaagagg
     2461 ctgtgcagcg ccgggggatt cgtgcctgct ggagccgctt ccctcagatc attccctgcc
     2521 gtagggcgtt atccctccag ccgcgaccgg agaatagtct ttatttcttc cgctgttttt
     2581 ttctgcagaa tatctcgggc aagatgtttt gcatcttccc gattcagcgc ggctatgcgt
     2641 gcccgaatta acggaatgga aacagatgcc atcgaaaaag aagagacacc cagcccgagc
     2701 aaaagttccg tgtataaagg atcaccggcc atttctccgc atatactgca gggaatgtta
     2761 tgagccgcag ccgcagaact tacatttgca atcatacgaa tcaccgccgg gtgagccggc
     2821 tcgtagagat gggctatttt ttcattgacc ctgtccaccg caagaaggta ctgaatcagg
     2881 tcattggtgc ccaggctgaa aaaatcgcat tcttcggcga tctggtcggc ctgtacggca
     2941 gcagaaggca cttcaatcat ggagccgact ttgagatccg ggttataggc tacgccttca
     3001 gaagacaatt cctggcgtgc ctcctgcaat atggcttttg cgtggcgaag ctcttcaagg
     3061 ctgctgatca tcggaaacat gatgcgtata ttgccctgag cacttgcccg gagcatggcg
     3121 cgcagctgca ctttaaaaat atccggatag gcgagactca accgtatagc gcgcagcccc
     3181 agctgcgggt tgctgtcttt atagttctga aggtacttca caaacttatc gccgccaata
     3241 tccatggtgc gaagggtaac gggcgccgga aacagttccg aagccacatg aagataagcc
     3301 tggtactgtt cctcttccgt gggcagtgtg tcacggttga agaaaagata ttccgttctg
     3361 taaagtccga cgccctgcac attcattttt aaactgtgct tgatttccag aggcagttca
     3421 atattgcttt cgagggttac ggcagtgccg tctttcgtgc aggcaggacc ggacaacagg
     3481 ctttccagca gatgggattg tatggattgc aggcgctgct gttctgtccg gtatttttca
     3541 accgtttcct gcgaagggtt gatgattacg acaccgctgg tgccgtcgag aatcataaga
     3601 ctgcccggcg acagtgattt ttgggaaatg gaaattccga caacagcggg tatttccaat
     3661 gcgcgggcaa ggattgccga gtggctggtc gggctgcccg tttcaaccag cagtcccagc
     3721 gctttgtcca gatcaagaga cgctgtgtcg gagggggcaa gatcctgagc aaccagtatg
     3781 ctcggctcag aaatgtgccg gagcaagggc ccgtcagatt taagaagatg ccgttgcaga
     3841 cgatccacaa cgtccagcaa atccgctgtg cgctcacgga aacgctgatc gtcgatatta
     3901 ttcatcattt ccgtgtattt cgaggctatg ctgctcagga taaactctac attgagtctt
     3961 tcttgttcca tctgtgcagc aaggtcgtca cgcagtgcca cgtcgtcaag gatcatcaga
     4021 tgcacgcgga aaatttcagc atgcgctttg cccacggcat cgacagtttt tttataaatg
     4081 gaagtcagct cacgacggac tttagcgacg gcttcatcaa gacgcatcag ttccgcctgc
     4141 acgtctgtta tgggacggcg agggacctca aagctggcac gggaatagaa cagcgccgga
     4201 gctatcgcaa tccccgggga cactcccata ccttgtaaat aaatttccaa aaaactatac
     4261 tcctgacacc ttagtcggat tttatttcat tgaagcccga ttcaaaaatt gctttgaccc
     4321 gtgccatagc ttcatcggca tcttcgccga tacaggacac cgatatcctg ctcccccggg
     4381 cggcaccaag ggtaagcagc cccataatac tttttgcgtt gacccgactt ccgttaaaaa
     4441 gcaggtatac atcgctttgg aaatgggcga tactttgcgc cagaagcgca gccggcctcg
     4501 catggatgcc aagtgggttg atgacaatta catcttcaag cagctcagac atctttaata
     4561 acccttaatt ctccaaacac tgccatacaa aaatttctgt aatactacga ttcagttgat
     4621 tgcacttgaa tgttaaggaa ggggcgaccc gctataagcg atgcttgtaa agcaccagca
     4681 gatcctgccg cacgaaagac agaaacaagc gcttctgtcg ggttgggctg aggaattact
     4741 gttttctgcc tgtaccaaaa cggcctgatt atcttgtctt tttttacgca gcccaaaata
     4801 tcgtgtttta cacgctctct gattttgcta ataatagcat atttgacagc caaaagtcgc
     4861 agagggcgca ttttat
//
LOCUS       DAOPWB010000190         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_17487_length_4868_cov_5.816746, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000190 DAOPWB010000000
VERSION     DAOPWB010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17487_length_4868_cov_5.816746"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PKN92_12460"
     CDS             <1..1262
                     /locus_tag="PKN92_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ49647.1"
                     /translation="ERASILARTTEQLADGTLYFEFLEAELKQHYSADSGEYYQVWTR
                     SGESVGRSPSLGEESLPVRLPLYQDPVFQSIRLPDGRNGRLVALSFLPHEEEAPAVDT
                     FVMAFAVPLTELETIISRLLQGLFFSGLALLLGLFPGIWLSVNRSLESLDLLSSQTST
                     IRTDDLSFRFPASEMPQELRPISLRLNDLLDRLQAAFERERRFTGDAAHELRTPIAEL
                     KILAEVGLAEAVSDMPSFRSYFEDALAIARHMESLVSLLLSLSRCQSGQQVLEKVHCS
                     SIGALLKEVCQRYQGEAEERRLSLELAMEEDFSAESDPVLLAAVFENLLSNAVVYTPA
                     GGRIRLAVLSGSGLREILIGNAVSGVTLSDVSRFTEPFWRKNQTFSATAHAGLGLALV
                     SAYARLLQIELAFSLPEPGWLQCTLRF"
     gene            complement(1259..2017)
                     /locus_tag="PKN92_12465"
     CDS             complement(1259..2017)
                     /locus_tag="PKN92_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNZ49648.1"
                     /translation="MSSSRYLQKSPAAELMAVMLLLPLAAIVAAILSEYSGLDLFLAR
                     LFFNEAAQNWPLKSNVITAFILHKLPQKMLALFAVLLLLHAAVSCFYAKWRHWCRVSA
                     YLLVSLLLCTGAVALMKHSTHIQTPWSLILFGKDMPYIRFFDQVPAGTPIGNAFPGGH
                     SSGGFAFISLYYLAGACCPEYRFRGLFFGLFLGTLFAVTQEVRGAHFLSHDLFSLALC
                     CLISGALSFLFFGAPKKDRRLSEEEESDPETHGL"
     gene            complement(2094..3962)
                     /locus_tag="PKN92_12470"
     CDS             complement(2094..3962)
                     /locus_tag="PKN92_12470"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ49649.1"
                     /translation="MSDLWIKSAAVLLLAAIIFSVNVSGYDLWPPDEPRYALVAREML
                     STGDYFLPKVNNEGYKEKPPLFFWCIAACAKLTGSVGPLSARMPSILAALTVLIFTTL
                     LARALFSDRIAFWSLLILMTTQRFWWNARFGQIDMLLTACLTAGLYMYWRWEKTGRKG
                     WILLFYLFMTAGLFSKGPGVLVFPVLFVLTRTWRSPHRMRHWIGLAAGCSFCVLLYAL
                     WAVPAHLAFAQEAGSDAGAVLGSNLLRQTLGRFFLGVSHANWPWYYLTTLPVDWLPWT
                     LFLPWIIYWVWTHRKDTPAISFLLSWTLPAFIFFTIAIGKRGVYLLPLFPAWAPFFAA
                     GILDFMDKAVIHWRRRLGWIYGIALILLGLAPAALLFTEHRNSWSPGLLPISLAAFVC
                     TATLIPLSGKNKLSFLPQQIAGSFLLIYLLTAILILPVINQHKSARFFCTPIAELADK
                     GVDFDLFSVGFAREEYIFYSRHFFKELHTEAIPLQAREDLSPMQSLKLQKKLARALVK
                     KAGKVPVQDMGKIRPDELERLQEAVRQALDQEDCPIQLAEEFKEGLEKENAILFDAFD
                     SPKPAFLYVQDEDWRWIYAIHPDVRNAVMLSQANVGSRHVLLFANPAGAHLFYALN"
     gene            complement(3955..4752)
                     /locus_tag="PKN92_12475"
     CDS             complement(3955..4752)
                     /locus_tag="PKN92_12475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ49650.1"
                     /translation="MKILVSVVVPAYNEAACLPPLVHAVHAAFEPLKSYDAEYIIVND
                     GSTDDTAGVLRELALHCPRLRCLHMTRNSGQSAALIAGMRASKGSIIVTLDADLQNDP
                     ADIPALLEALQEGDCVCGYRKKRNDTAFRRLTSYLGNRVRRLIVDDGVRDAGCGLKAF
                     RRNCLDFIVPFHGVHRFFAAMVRNGGLTVTEVPVNHHPRKQGKSKYGFFNRFFWVMQD
                     FFGVAWLKKRYLLISFEEDAEKNEQESQNQEPLTQATTSRDGEPTHV"
BASE COUNT         1170 a         1256 c         1389 g         1053 t
ORIGIN      
        1 tggagcgcgc ctctattctt gcgcgaacta cggagcagct tgcggacggt accctctact
       61 ttgagtttct cgaggcggaa ctgaagcagc attattcagc agacagtggc gagtactatc
      121 aggtctggac acggagcgga gaatcggtgg ggcgttcccc ctctctggga gaggaaagcc
      181 tgcctgtgcg tctgcctctt tatcaggatc ctgtttttca aagcatccgg ctgccggacg
      241 ggcggaacgg acggcttgtc gccttaagct ttctgcctca cgaagaggaa gcgcctgcag
      301 tcgacacctt tgtcatggcg ttcgccgttc ccctgaccga attggaaacg atcatcagcc
      361 gtcttcttca ggggctcttc ttttccggac ttgcccttct gctggggctt ttccccggca
      421 tctggctgag cgtgaaccgt tctctggaga gtctggactt attgagcagt cagacaagca
      481 ccatcaggac ggatgatctg tctttccgtt ttcccgcatc ggaaatgcct caggaactgc
      541 ggccgatcag tctgcggctg aacgatcttc ttgacaggct gcaggcggcc tttgaaaggg
      601 aacgtcgttt caccggcgat gccgcgcatg agctgcgcac gcctattgcc gagctgaaaa
      661 tcctggctga agtcggtctt gcggaagcgg tttccgatat gccgtctttt cgctcctact
      721 ttgaagatgc gcttgcgatt gcccgccata tggaatcgct ggtatcgctg ctgctttccc
      781 tgtcccggtg ccagtcgggg cagcaggttc tggaaaaagt acactgcagc agtattggtg
      841 cactgctgaa agaggtttgc cagcgctatc agggggaagc ggaagaacgg cggctttctc
      901 ttgagctggc aatggaagaa gatttttctg ctgagagcga tcccgttctt cttgctgccg
      961 tttttgagaa tcttctttcc aatgcggtcg tgtatacgcc tgcgggtggc cggatccgcc
     1021 ttgccgtcct cagtggcagc gggctaagag aaattctgat cggcaatgcg gtatccggag
     1081 taaccctgtc ggatgtatcc cgcttcacag agcctttctg gcgaaagaac cagacctttt
     1141 ccgctacggc acatgcagga ctggggcttg cgcttgtttc cgcctacgcc cgcctccttc
     1201 agattgagct tgcgttttct cttccggaac ccgggtggct tcaatgtacc ctgcgctttt
     1261 aaagcccgtg cgtttccggg tctgattctt cttcttcgga aagccggcgg tcttttttcg
     1321 gagcgccgaa aaacaggaag gagagcgctc cgcttatgag acagcacagg gcgagagaaa
     1381 ataaatcatg gctcagaaaa tgagcgcctc tcacttcctg cgtgacggcg aagagtgtgc
     1441 ccagaaacag accgaagaaa agcccgcgaa accggtattc cgggcagcag gcgcctgcca
     1501 gatagtataa actgatgaaa gcaaatcctc cggaagagtg accgccgggg aaagcattgc
     1561 ctatgggtgt gcctgccggc acctgatcga aaaaacgaat gtagggcatg tctttaccga
     1621 aaaggatcag actccagggc gtctggatat gagtgctgtg tttcattagc gcaactgctc
     1681 cggtgcagag aagcagagaa accagcaggt aggcgcttac ccggcaccag tgccgccatt
     1741 tggcgtaaaa gcaggaaaca gccgcatgaa gcagaagcag cacagcaaaa agagccagca
     1801 tcttctgcgg aagtttatgc agaataaagg ctgtaatcac attggacttt aaaggccagt
     1861 tctgtgccgc ctcattgaaa aacaggcgcg ccagaaaaag gtcgagaccg gagtattcgg
     1921 acagaatggc ggcgacaatc gctgccagag ggaggagcag catgaccgcc atcagttctg
     1981 cagcagggct cttttgaagg taccggcttg atgacatggg aaggttatcc tgggttagat
     2041 ccaatggatc ggtttcaaaa gcacggcaga tcctgagata gggtgtcggt gtttcagttc
     2101 agtgcataaa agaggtgtgc tcctgccgga ttggcgaaga ggagtacatg gcgactgccc
     2161 acattcgcct gtgacagcat aacggcattg cgcacatcgg gatgaatggc atagatccag
     2221 cgccagtctt catcctgaac gtagagaaag gcaggtttcg gtgagtcgaa ggcatcaaaa
     2281 aggatggcat tttccttttc cagcccttcc ttgaattctt cggcaagctg gatggggcaa
     2341 tcttcctgat ccagtgcctg acggactgct tcctgcagcc tctccagttc atcgggccgg
     2401 attttgccca tgtcctgcac cgggactttc ccggccttct ttaccagtgc acgggcaagt
     2461 tttttctgga gcttcaggct ctgcatcggc gacaaatctt ctcttgcctg cagcggtatg
     2521 gcttcggtat gcagctcttt aaaaaagtgt cttgaataaa agatatattc ttccctggca
     2581 aagccgacgg aaaacaggtc aaaatccact cctttgtcgg cgagttcggc aataggcgta
     2641 caaaagaagc gtgcggattt atgctgatta atgacaggca ggatcagaat ggctgttaaa
     2701 agatagatca gcaggaaaga gccggcgatt tgctggggca gaaaagagag tttattcttc
     2761 ccggagaggg gtatcagcgt ggcggtacag acaaaggcgg ctagactgat ggggagtaaa
     2821 cccgggctcc acgaattccg gtgctccgta aagagcagtg ccgcaggggc aagtcccagc
     2881 aggattagag ctatgccgta gatccagccc agccgccgcc gccagtgaat gacggcttta
     2941 tccatgaagt ccagtattcc ggcggcaaaa aagggtgccc aggcgggaaa aagcggaagc
     3001 agataaacgc cccgctttcc gatggcgatc gtgaaaaaga taaaggcagg cagtgtccag
     3061 ctcaacagaa aggatatggc aggcgtgtcc ttgcgatgcg tccataccca atagataatc
     3121 cagggaagaa aaagcgtcca gggaagccag tccacaggca gtgtggtgag gtaataccac
     3181 ggccagttgg catggctcac gccgagaaaa aagcggccca gtgtctgacg caggaggttg
     3241 gatcccagca cggcgccggc atcgcttccc gcttcctgag caaaggcgag gtgggcaggc
     3301 accgcccaga gtgcatacag cagcacgcag aaggaacagc ctgccgccag accgatccag
     3361 tggcgcatgc ggtgcggact gcgccaggtt cgggtcagca cgaaaagcac agggaaaaca
     3421 aggacgccgg gaccttttga aaaaagcccg gcggtcataa aaaggtagaa gaggagtatc
     3481 caacccttgc ggcctgtttt ttcccagcgc cagtacatat acagcccggc ggtcaggcag
     3541 gcggtgagca gcatgtcaat ctggccgaag cgcgcattcc accagaagcg ctgggtcgtc
     3601 atcagaatca gcagcgacca gaaggcgatc cggtcggaaa acaaggctct tgccagcagc
     3661 gtagtgaaaa tgaggacagt caaagccgcc agaatagaag gcattcgagc ggacagaggc
     3721 cctacgcttc cggtaagttt ggcgcaggcg gcaatgcacc agaagaaaag cggcggtttt
     3781 tccttataac cctcattgtt tactttgggc aggaagtaat cgccagtact gagcatttcc
     3841 cgtgcgacaa gcgcatagcg gggctcatcg ggcggccata gatcatagcc gctcacattc
     3901 acagaaaaaa taattgccgc cagaagcagc acggcagccg attttatcca gaggtcagac
     3961 atgggtcggt tctccatcgc gggatgtggt tgcttgtgta agaggttcct gattttggct
     4021 ttcctgctca tttttctcag cgtcttcttc gaaagaaatg aggaggtagc gttttttcag
     4081 ccaggcaaca ccgaaaaagt cctgcattac ccagaagaag cggttgaaaa agccgtattt
     4141 ggattttccc tgcttgcgtg gatggtgatt taccggcact tctgtaacag tgagccctcc
     4201 gttacgcacc atggcggcaa aaaaacgatg gacgccatga aaaggaacga taaagtcaag
     4261 gcagttccgc cggaaagctt tgagcccgca gcccgcatcc cggacaccat catccacaat
     4321 caggcggcgc acccggtttc ccagatagct cgtgaggcgg cggaacgccg tgtcattgcg
     4381 ctttttccgg tatccgcaca cgcagtcacc ctcctgcaac gcctccagaa gagcaggaat
     4441 atcggcaggg tcattctgga ggtcggcatc cagggtgaca ataatgcttc cttttgaagc
     4501 gcgcattccg gcgataagcg ctgccgactg gccgctgttc cgtgtcatgt gcaggcacct
     4561 gaggcggggg caatgcaggg caagttcacg cagcaccccg gcagtatcgt cggtacttcc
     4621 gtcattgact ataatatatt cggcatcata gctttttaag ggctcgaagg cagcatgtac
     4681 ggcgtgtacc agcggcggca ggcatgccgc ttcgttatac gccggaacga caacagaaac
     4741 aagaattttc atgatcatac aatccgaaca ggttcaaaca tgggggtcac tgtttgcctc
     4801 agtatacaga tctgataatt accgaaagat tacgaaatac aataagtata tttttatgca
     4861 agacctgc
//
LOCUS       DAOPWB010000191         4860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_17508_length_4860_cov_5.094485, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000191 DAOPWB010000000
VERSION     DAOPWB010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17508_length_4860_cov_5.094485"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKN92_12480"
     CDS             complement(<1..228)
                     /locus_tag="PKN92_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNZ49651.1"
                     /translation="MRKTLNIESVRIAMQEQGLNQAWIAKEIGVTRAAVTKWLNLEDF
                     PRPDKLLKLGILLKLSFKQLVKESDDPMEPVV"
     gene            complement(834..2069)
                     /gene="gntH"
                     /locus_tag="PKN92_12485"
     CDS             complement(834..2069)
                     /gene="gntH"
                     /locus_tag="PKN92_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis MBL fold
                     metallo-hydrolase GntH"
                     /protein_id="HNZ49652.1"
                     /translation="MKTDFTRRDMLKLSALGAGSAVVAGGLTGCPVIPPQEVFPIGEP
                     LDENEMRITFMGTSVVPRQKQECNSVFVEVGSGDSFVFDCGSGVCSKYNAMGVTPARM
                     DKIFLTHLHGDHTSDIVTIYCFGPSQDRKWPLQVWGPSGDSAEDGTNAFCANLARMMH
                     WHIESFSFGRTGTIGLGDGYDIIPTELPYMTVGGVAYDNPETGVRITHFPAVHCRNGS
                     ISYKLEWNGMSMIFSGDTKPNDYMIQQGQGVDVLIHEMVVPPEVWASKNSGLKEGDPG
                     YPQALAYAKAVQDSSHTPQKALGYIFAQTRPRLGVPTHFQVNPDTVGPAMEDIRLFYD
                     GPVTIATDLLVLNVSKEKIRQRTAIIDEWAWYAQPIIPNPATLMPPRYPSPTAQLSEE
                     LLSHSIPEELYDPQVSAKV"
     gene            complement(2225..2887)
                     /locus_tag="PKN92_12490"
     CDS             complement(2225..2887)
                     /locus_tag="PKN92_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNZ49653.1"
                     /translation="MSDPKTKSSSSFAIRIAIVDDHPAVREGLALLLHPKGIVVCAEA
                     GSCEEALPAVLREKPDVVLVDLSLGSEDGTALLEKLHNNHFPSLVYSMYEDGRKITDA
                     FLAGAQGYVTKREVHGVLVHAIAEVAQGRRYVSPRAAVALAEYAVSRPKDNPLTELSV
                     QEQQVYRLLEQGKSTRSIAEAMGISVRTVESYCERICVKLDLEGMRALRVFAESHHSE
                     FR"
     gene            complement(2884..4191)
                     /locus_tag="PKN92_12495"
     CDS             complement(2884..4191)
                     /locus_tag="PKN92_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNZ49654.1"
                     /translation="MLKRDLMEDISADAAFAAWRTRAAGILTFANTMVFLPVVLIVAA
                     GQGPPLTLFTKISVYLFYGAILSNVLFWKRQPEIRIGINLAVGYLIAFVVGIALPQGP
                     FIKTLPLVLPLIAMVFFEKKAGYGAALLSAAMLFVSPLLSTLSFLSGILVDPSIYKPS
                     VPPFVWFQAVAQTAILLQLMLLLNQFHNLLKQTLTERARASQNLTDEMQKRLVLEQEL
                     ARHADEERRRLGCEIHDGICQMITASLLRAEALAGQLEPMDAAVEKSLRGITSLLEES
                     LNEAHVVAQGLCPLQPGPDALPSALRRLAGRIQSTAGIDCILQCSDNADPGDSTKENH
                     LFRIAQEAVSNAVKHARAGTILISLTGDRHFIELRIEDDGVGLSDAGTHEGMGLQTMS
                     YRAHLMEGELTAASIETGGTRILCRVPRHLQPKSGFTKEQAAS"
     gene            4589..>4860
                     /locus_tag="PKN92_12500"
     CDS             4589..>4860
                     /locus_tag="PKN92_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HNZ49655.1"
                     /translation="MPKIHETAIVHPGAELADDVEIQPFSIIEAEVVLGPGCIIGPHC
                     VIGKGTVMGANNRTFSGAQIGIPPQDLKHLSDRAGKTMIGDGNTFR"
BASE COUNT         1167 a         1303 c         1227 g         1163 t
ORIGIN      
        1 caccacaggc tccatcggat catctgattc cttaaccaat tgtttgaaac tgagtttaag
       61 caatatgccc agtttcagga gcttgtctgg tcttggaaaa tcctccaagt tgagccactt
      121 ggtcactgcg gcacgagtga ccccgatttc tttggctatc cacgcttgat taagaccttg
      181 ttcctgcatt gcgatccgca cggattctat gttgagtgtc tttctcatgt ctatatcata
      241 tccgatttgt taacttttgt caacttttgg ttcggataaa cggcggattc aatagtgaag
      301 cgctgttttg ctttgaatga atgcttcagc aggtgtgcag gaatccaagt tttttcctgg
      361 cctgttattc aatttatcaa cgacccgctg caggtgcttc ggatcaattt tatcaaaact
      421 ttttttagag aaacaatgtc gtagaaggac gccagtactg tcttttctgt ctctgccgtt
      481 tgagcagcaa ggccttgcta agtgcaccgc aatccccatc ttttgtgcta ttttttatgg
      541 tctgcaaatg cagttccatt agcaaatggc aattgaaaat aaactctttt ctttaaagaa
      601 cctcagaaga tggataataa cctctgtcgc caacccggca gaaaagcgcc tttcgatcca
      661 acaggctccc ctttcccagc acaaatacag gaggaatggc tttccctgga gatcgggttt
      721 cagatactga aaattagaag caggccagga aaaacctgtt ccggcttcct gacctgcttt
      781 tttgcatgat actggaaagg tgcgatgagc accggtgtat gcgcagcgaa cccttatacc
      841 tttgcagaca cctgcggatc gtagagttct tccgggatgg aatgggacag cagttcctcg
      901 ctcagctgcg ctgtgggcga aggataacgg ggcggcatga gtgtggcagg attcgggata
      961 atcggctggg cataccacgc ccactcatca atgatcgctg ttctctggcg gatcttttct
     1021 ttggagacat tcaatacgag aaggtctgtc gcaatcgtga caggtccgtc ataaaaaagc
     1081 cgtatatcct ccatggcggg tcccactgta tccgggttga cctgaaaatg ggtaggcaca
     1141 ccaagtctcg gacgggtctg agcaaaaatg tagcccagtg ccttttgcgg cgtatgcgaa
     1201 gaatcttgta ctgccttggc ataggcaagc gcctggggat agccgggatc gccttccttg
     1261 agtccggagt ttttggacgc ccagacctcc ggcggaacaa ccatttcatg aatcagcaca
     1321 tccacaccct gcccctgctg aatcatataa tcgttgggct tggtatcgcc gctgaaaatc
     1381 atggacatgc cattccattc cagtttgtaa ctgatggatc cgtttctgca gtgaaccgcc
     1441 gggaaatgcg taattctgac gccggtctcc ggattatcat aagcaacacc accgacagtc
     1501 atatagggca gttccgtcgg tatgatatcg tagccgtcgc ctaaaccaat ggtaccggta
     1561 cgaccgaagc tgaaactttc aatgtgccag tgcatcatgc gggcgagatt ggcgcagaac
     1621 gcatttgtcc cgtcttcggc gctgtctccg gagggccccc agacctgaag cggccatttc
     1681 ctgtcctgag atggaccgaa gcagtaaatt gtcacgatgt cgctggtatg gtcgccatga
     1741 agatgggtca ggaaaatctt gtccatgcgg gcaggcgtaa ctcccatggc gttgtacttg
     1801 gaacagacac cggagccgca gtcgaaaacg aaactgtcgc cactgcccac ctcgacaaaa
     1861 acgctgttgc attcctgttt ctgccggggc acgacagagg ttcccatgaa agtgatgcgc
     1921 atttcatttt catcgagtgg ctccccgata ggaaacactt cctgcggcgg aatcacgggg
     1981 cagcctgtca ggccgcctgc cactacggca ctgcctgcac ccagtgcgga cagcttcaac
     2041 atgtcccttc gtgtaaaatc agttttcatc ggttactctc ctttgttgaa actcatacga
     2101 tgaaagggaa acacctggtt tcttttttca ggagaattat agggagtttt ttttaatctg
     2161 tatgccgtgg aacccggaca tttctgtccg ggaaacccgg acatgctttg catcggagca
     2221 aggactaccg gaactcacta tggtggcttt ctgcaaaaac acgcagtgcc cgcatccctt
     2281 ccagatcgag cttcacacag atgcgctcgc aataggactc aactgtccgg acactgatcc
     2341 ccattgcctc ggcaatgctg cgggtggact taccctgttc cagcagacgg tagacctgtt
     2401 gttcctgaac actcagttcc gtgagcgggt tgtctttcgg ccgggacacc gcatattctg
     2461 ccagtgccac agcggcgcgg gggctcacat agcggcgacc ctgtgcaacc tccgcaatgg
     2521 catgcaccag aacgccgtgc acttcccgtt tggtcacata gccctgagcg cccgccagaa
     2581 aggcatccgt gattttgcgc ccgtcctcat acatggaata tacaagagaa ggaaaatgat
     2641 tgttgtgcaa tttttccaga agtgcggtac cgtcttccga acctaaggac agatcaacca
     2701 gtacgacatc cggtttttct cttagaacag caggcagcgc ttcttcacag gagcccgcct
     2761 ccgcacagac gacaattcct ttcggatgaa gcagcagcgc caatccttca cggacagccg
     2821 gatggtcgtc cacaatggca atgcggatgg caaaagagga ggatgatttt gtttttggat
     2881 cactcatgat gctgcctgct ctttcgtaaa accggatttc ggctgaagat gtcgcggcac
     2941 ccggcacaga atacgggtac cgcctgtctc aatagatgcc gccgtcagtt cgccttccat
     3001 caggtgcgcg cggtaggaca ttgtctggag ccccatcccc tcatgcgttc cggcgtccga
     3061 cagcccgaca ccatcgtctt caattcgcag ttcaataaag tgtctgtcac ctgtcaatga
     3121 aatcaggatg gtgcctgccc gggcatgttt cacagcgttg gataccgcct cctgcgcaat
     3181 acggaacaga tgattttctt tcgtgctgtc ccccggatcc gcattgtccg aacactgaag
     3241 aatacagtct atacctgccg tcgactgaat tcttccggca agccggcgca gggcggaggg
     3301 cagcgcatcg ggacccggct gcaaagggca tagcccctgt gccaccacat gcgcctcatt
     3361 cagactttct tccagaagcg acgtgattcc gcgcaacgac ttttcaacag cggcatccat
     3421 cggttccagc tgacctgcca gcgcttctgc ccgaagcagc gaagcggtaa tcatctggca
     3481 tattccgtcg tggatttcgc agcccaggcg acgacgttcc tcatcggcat gacgcgccag
     3541 ctcctgttca agcacaagcc gcttctgcat ttcatccgtc agattctgag aggcacgggc
     3601 gcgctcggtc agggtctgct tcagcagatt gtgaaactga ttcagcagaa gcatgagctg
     3661 cagcagaatc gctgtctggg caacggcctg aaaccagacg aagggcggta cagacggctt
     3721 gtagatcgac gggtcaacca gaattcctga aaggaaagaa agggtgctca gaagcggaga
     3781 aacgaacagc attgccgcgc tcagcagcgc cgccccatag cctgcttttt tttcaaagaa
     3841 gaccatggca atcagaggaa gtaccagtgg cagtgtcttg ataaagggac cctgtggcaa
     3901 tgcaatcccc acaacaaaag caatcaggta ccccacagcc agattaattc ctatgcgaat
     3961 ctcgggctgc cttttccaga aaagaacatt ggacaaaatg gcgccgtaaa aaagataaac
     4021 acttattttg gtgaagagtg ttagcggagg gccctgtccg gcagcaacaa tcagcacaac
     4081 aggcagaaag accattgtgt tggcaaacgt cagaataccg gcagctcggg tgcgccaggc
     4141 agcaaaagcg gcatcagcgg aaatatcttc catcaaatca cgtttgagca ttgccggcag
     4201 actccctcta ttcagcatga acagaatact gctattttaa atcctcgggc accggcagat
     4261 acacttttta tcggaacata ttcgggcagg gcatttccgg aagacttatt gttgtgtact
     4321 cttttctttt caacacagaa gaaatccatc agaataacca atgagcgggc ctgctggaaa
     4381 ccctgtttta catcctgacc gacagagggc tccccactgc ccggacttcc ctgtttggtt
     4441 taaatgcctg aattgtggta ttaaatggtg catacgcgtt gccctccatt tttgaagttg
     4501 tgcttttcaa agataccaac cgaggggtaa acgatttctt caggtaatca gcgaggacgg
     4561 tacctgtcct gttattcgaa agagtggcat gcccaaaata catgagacag ccatcgtgca
     4621 ccccggtgcg gaacttgccg atgacgttga gattcagcca ttcagcatta tcgaggcgga
     4681 ggttgtgctc ggccccggct gtattatcgg ccctcactgc gtcatcggaa aaggaaccgt
     4741 gatgggtgcc aacaaccgca ctttcagcgg ggcacaaatc gggattcccc cgcaggatct
     4801 gaagcatctc tccgacaggg cgggcaaaac gatgatcggt gacggcaaca cattccggga
//
LOCUS       DAOPWB010000192         4849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_17558_length_4849_cov_5.321026, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000192 DAOPWB010000000
VERSION     DAOPWB010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17558_length_4849_cov_5.321026"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..998
                     /locus_tag="PKN92_12505"
     CDS             171..998
                     /locus_tag="PKN92_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNZ49656.1"
                     /translation="MMLSDYMLPGISLVAFWLFVSIAVIIQGISKSGFAGGAGVLSLP
                     LMMLVMPVDKVVASLLPLLILLDFNAIYHHRHNRDWQVIRMIYPPALIGIILGALVWW
                     WIGRAGLASYEGYIKRFVGVIAIFLAFYILGKELSLKWVQGKKAGRKIGWAAGLFAGF
                     SSTIAHAAGPVVSLFVFSQDLGKTLFVGTVAWAFTLINLTKLPFYVAADLIDFSVLHF
                     DLYLLPLVPLGSWLGKWLHHRVSEKVFNRIIMGLTLLAGIQLLFNVNLVRSFLAWFQ"
     gene            1148..1579
                     /locus_tag="PKN92_12510"
     CDS             1148..1579
                     /locus_tag="PKN92_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49657.1"
                     /translation="MTFNELRKGERWMTEEELRLIRRYVLCMKNFLIARGVEESPLLC
                     FRVGDLGLQWLIVRRLENGLAQQENCEKTELPLATTALADHIGKGRERLRKAIRELED
                     TCARLGTPLDTGLADQLAPLMRETRALLHDPAFSPEALDSD"
     gene            1640..2101
                     /locus_tag="PKN92_12515"
     CDS             1640..2101
                     /locus_tag="PKN92_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNZ49658.1"
                     /translation="MQYRVSFGTLLLLGLALLLVSSGCATRPKPPRGVRAFDRQMEVT
                     AYDAGKKSTNWKRNWLLQPVVASGPDKGKRKKVGITASGTKAAPGTLAADTNHYPFGT
                     IMYIPGYGYGRVEDRGSAVKGPDRVDVFFKSRKEALRWGRQKLKVRVWPVR"
     gene            complement(2108..2953)
                     /locus_tag="PKN92_12520"
     CDS             complement(2108..2953)
                     /locus_tag="PKN92_12520"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNZ49659.1"
                     /translation="MMRKISLLTCSLSLLTTLLFITGCAGLERKVKVEILPDIPYARD
                     DGSGVPLLMDIVQGQKPKKLPRHAIVMVPGGNIPEGARKQEAQRKLAHALAQKGYVCF
                     LIDYRMPLLDVPPEMEKKIPFTDRIRPAAMDVKKALRQVRDHADGYQIDPDAIALLGD
                     SAGSVIALAAGVSPPDAFIEEGETPVKVAAIINLWGTADYFPELFGTHTPPIMSVHGG
                     QDYFIGYSLTPALNIDTLCKKHMIPHYFFPVPTAQHGAWQAVVDDRTLPELIDFFLTE
                     VTGAP"
     gene            3029..4501
                     /locus_tag="PKN92_12525"
     CDS             3029..4501
                     /locus_tag="PKN92_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49660.1"
                     /translation="MEHEKYSSLASFLGSCGVDREGLTQWLDIASRERFARAHFRDRN
                     GAPWQVRPYQRPSLESRALRKVHCDGRDVGKTAEIEIIACWAMVACPDREMLIATQCE
                     NHLFPLMNRLARRFQQTPAFTPSLVEMRRSPSWHFRFSNNFVLWGRIAGPRGMNFQGM
                     HVDWQIVDEAQEMTESGWGELYQALNGGGRRWVYGVPNGLRNSFYRMTCQPDAEHYNW
                     SSRENPEFTDEKDQELIRLYGGRNSPGYIHRVLGQHGEPSHAVFSLDEYLSCVDSSRP
                     CCSFSLQEGDSFEAPEGIALGRFYLGCDLGYARDPSEFVVYRDAPPWLIQECRVHLEK
                     MNYKRQEEIIVELDRAYRFSAIGIDAGNSGRAVAHMLMSRGQDWCARVHALEFGAMVD
                     LHLMPDGRRARRRAKEFMTELIQRRLSERTLIFPPDPDREAQYAAHTYSVNAQGQIVY
                     EKGDDHLIDADRCALFCHYLDQREPEPAFRSSLAPEVLLF"
BASE COUNT          971 a         1208 c         1476 g         1194 t
ORIGIN      
        1 attcccttcg ccggctccat gctttcccct tgcattcaaa agcggtatac tgctatgggt
       61 gcaggggatt tttttgtcct tttgcctttg catgtgccgg ggactctgtt ttaatatcag
      121 atccgggctg tcaccgaagt tcccgtcgtt ttttttcaat gaaagtgctg atgatgctct
      181 cagactatat gctgcctggt atttcactgg ttgccttctg gctttttgtt tccattgccg
      241 taataatcca ggggatcagc aaatccggtt ttgcgggcgg tgctggcgta ttatctctgc
      301 cgctgatgat gctggtcatg ccggtggaca aagtggtcgc ctcgctgctg cctcttttaa
      361 ttcttcttga cttcaatgcc atttatcatc accggcacaa ccgggactgg caggtcatcc
      421 gcatgatcta tcctcctgcg ctgataggta ttattctggg tgcgctggtc tggtggtgga
      481 taggccgtgc aggcctcgca agctatgaag gctatatcaa acgctttgtc ggggttatcg
      541 ctatttttct ggccttttat attctgggca aggaactttc tttaaaatgg gttcagggga
      601 aaaaggcggg caggaagatc ggctgggcgg cggggctttt cgccgggttt tcctcgacca
      661 tcgcccatgc ggcaggcccg gtggtcagcc tttttgtttt ttcgcaggat cttggcaaaa
      721 cgctttttgt gggtacagtc gcctgggctt ttacgctgat caatctgacg aaacttcctt
      781 tttatgtggc ggcggatctc atcgattttt ccgtgcttca ctttgatctt tacctgctgc
      841 cgctggttcc gctgggctcg tggctaggca aatggctgca tcatcgtgtt tcggaaaagg
      901 tcttcaaccg tataatcatg gggctgacgc ttcttgccgg tattcagctt ttgttcaacg
      961 taaatcttgt gcggagtttt cttgcctggt tccaatgaat gggccgtaaa cgcccagttt
     1021 accggttcag gcgccaccgc tttccctttt aatctgtatt ctctgtggca accgccctgt
     1081 tttctttgaa aaatgcagtg tttttctctg taaagtgagc gtctggctgc gctgacagga
     1141 ggaaaccatg acatttaacg aactgcgtaa aggggagcgt tggatgacag aagaggagtt
     1201 gagactgatc cggcgttatg ttctctgcat gaaaaacttt cttatcgccc ggggcgtgga
     1261 agaatcgccg ctgctgtgtt ttcgtgtggg cgatctcggg ctgcagtggc tgattgtccg
     1321 gcggctggaa aacgggctcg cccagcagga gaactgcgag aagacggagc tgccgcttgc
     1381 cacgactgct ctggcggatc atatcggcaa agggcgtgag cggctgagaa aagcgattcg
     1441 ggaactggag gacacttgcg cccggctggg cacccctctg gatacggggc ttgctgatca
     1501 gttggcgccg ctcatgcgcg aaacccgggc gctgcttcat gatccggcat tttcgccgga
     1561 agctctggat agcgactgaa ggcttttctg cggttttatg tcataataaa ggcagttcgg
     1621 aaccgacagg gagatcgcca tgcagtaccg tgtatcgttt ggaactcttc tgctgctggg
     1681 tctggcgctg ttacttgtaa gcagcggctg tgccacccgg ccgaagccgc cccggggtgt
     1741 ccgtgctttc gaccggcaga tggaagtgac cgcttatgat gccgggaaaa aatccaccaa
     1801 ctggaagcgg aactggctgc tgcagccggt ggttgccagc ggtccggaca agggtaagcg
     1861 caaaaaagta gggatcacgg cgagcggaac gaaagcggcg cctggtactc ttgccgccga
     1921 cacaaaccat tatcccttcg gcaccatcat gtatatcccc ggctacggct acggcagggt
     1981 ggaagaccgg ggcagcgcag tgaaggggcc ggatcgggtg gacgtgtttt tcaaaagccg
     2041 caaagaggca cttcgctggg gccggcaaaa actgaaagtg cgtgtgtggc cggtgcggta
     2101 acttcggtca gggcgcgccg gttacttccg tgaggaaaaa gtcgatcagt tcgggcaatg
     2161 ttctgtcatc gaccaccgcc tgccaggcgc catgctgcgc cgtcggcacg ggaaagaagt
     2221 agtgggggat catgtgtttt ttgcagaggg tgtctatatt cagtgccggg gtgaggctgt
     2281 acccgatgaa ataatcctgt ccgccgtgaa cactcataat gggcggcgtg tgtgtgccga
     2341 agagttcggg aaaataatcg gcagtgcccc aaagattgat aatggcagcc acttttacag
     2401 gcgtttcgcc ttcttctatg aaggcatcgg gcggtgacac tccggccgca agagcgatga
     2461 cggagcctgc gctgtcaccc agcagggcta tcgcatcggg gtcgatctga taaccgtccg
     2521 catgatcacg cacctgccgc agtgcttttt ttacgtccat cgctgccggg cggatcctgt
     2581 ctgtaaaggg gattttcttt tccatttccg ggggaacatc gagcaggggc atccggtaat
     2641 caatgagaaa acagacatag cctttctgtg caagtgcatg tgcaagtttt cgctgcgcct
     2701 cctgctttcg cgcgccttcc ggtatatttc cgccgggcac cataacgatg gcatggcggg
     2761 gcagtttttt gggtttctgc ccctgcacga tatccatcag cagcggtacg ccgctcccgt
     2821 catcacgggc ataggggatg tcgggcagaa tctccacctt cacttttcgt tcgaggccgg
     2881 cacagcctgt aataaagagc agtgtcgtca gcagagagag ggaacatgtc aggagggaaa
     2941 ttttacgcat catgtgggta atcatactct tttttaacag gatggaacat cttttttccg
     3001 gagcgccgct gtcgggggga gtctgctgat ggagcacgaa aagtattcga gcctggcgtc
     3061 ttttctcggc agctgcggcg ttgatcggga gggattgact caatggctgg atattgcgag
     3121 ccgggagcgt tttgcccggg ctcacttcag agacagaaac ggcgcgccct ggcaggtgcg
     3181 gccctatcag cgcccttcgc tcgaatcgcg tgccctgcgg aaagtgcatt gcgacgggcg
     3241 tgatgtgggc aaaacagcgg agattgagat tatcgcctgc tgggcgatgg ttgcctgtcc
     3301 cgacagagaa atgctgatag caacgcaatg tgagaatcat ctgttcccgc tgatgaatcg
     3361 tctggcgcgc cggtttcagc agacgcctgc ctttacaccc tctctggtgg aaatgcgcag
     3421 gtcgccgtcg tggcactttc gcttttccaa caattttgtg ctgtgggggc gcattgccgg
     3481 accacggggc atgaactttc agggcatgca tgtggactgg cagattgtgg atgaggcgca
     3541 ggaaatgacg gagtcgggct ggggcgagct gtatcaggca ctgaacgggg gtggacgccg
     3601 gtgggtgtac ggcgtgccga acggtcttcg caacagtttc tatcgtatga cctgccagcc
     3661 cgatgcggag cattacaact ggagcagccg ggagaatccg gagtttacgg atgaaaaaga
     3721 tcaggaactg atccggctct atggagggcg gaactcaccg ggttatattc atcgggtact
     3781 cggacagcac ggcgagccga gtcatgcggt cttttcgctg gatgaatatc tttcctgtgt
     3841 cgacagcagt cggccctgct gcagtttttc tctgcaggaa ggagatagtt ttgaagcacc
     3901 ggaagggatt gcgctgggcc gtttttatct cggttgtgat ctggggtacg cccgggatcc
     3961 gtcggagttt gtggtatatc gcgatgcgcc gccctggctg attcaggaat gtcgggtgca
     4021 tcttgaaaag atgaactaca aacggcagga agaaattatt gtggagcttg atcgcgccta
     4081 tcgcttcagc gccatcggca ttgatgcggg aaacagcggc agggcggtgg cgcatatgct
     4141 gatgagccgc ggtcaggact ggtgtgcccg ggtccatgcg ctggagttcg gcgccatggt
     4201 ggatctgcat ctgatgccgg acggcagacg tgcccggcgg cgcgccaaag aattcatgac
     4261 agaactgatt cagcggcggc tgtcggaacg gacactgatt tttccgccgg accccgatcg
     4321 ggaagcgcag tatgctgccc atacctattc ggtcaatgcg caggggcaga tcgtgtatga
     4381 aaaaggagac gatcacctga tcgatgcgga ccgatgcgcc cttttctgtc attatctgga
     4441 tcagcgcgaa cccgaaccgg ccttccgttc atctttggcg ccggaagtac tgcttttctg
     4501 aaaaagggca tgctggattg tttcattacg gtcggacgaa gggtgcgggg aaacacaatt
     4561 gtaaagcaat ccagtacccg catcgggcgt cagttctgtg cgcagagacg ccggagggtg
     4621 gacgtaacgg cggcgtgtac aggaatctta attcgggtat gggcgccttt gcttcacagg
     4681 actgcattcc acaccaaagt gagtggacac ggtggacaca gtggacacgg tggactgtgc
     4741 attcggtgta tggccttagc cttatttcct acctgagact tatatgggtt ccgcaccaaa
     4801 atgctctggc tcaaaacata aaatccgcca gcctgggaac gccaatcga
//
LOCUS       DAOPWB010000193         4831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_17632_length_4831_cov_4.388191, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000193 DAOPWB010000000
VERSION     DAOPWB010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17632_length_4831_cov_4.388191"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            470..1531
                     /locus_tag="PKN92_12530"
     CDS             470..1531
                     /locus_tag="PKN92_12530"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNZ49661.1"
                     /translation="MSDTPSLRIAVLKGGMSLEHEISLLSAQGVEAALESLGYDIVPV
                     LITRTGEWQVEGRKKEPALDGLKRLKKSGVDCVFLALHGANGEDGRIQGLLDLLNMPY
                     TGSGCSASALAMDKPLAKTVVAAAGVQVAPHLCFTPSLWQKEKRKLPTISKALSFPMV
                     VKAPCQGSSCGLAIVANEKELAQAIEEIMPMEQRVMVENFVKGREFTCAVLDLIGSNA
                     MEALAVTEIRPITSAFFDYHAKYTPGATEEITPAPLRAAVTKAIGVMACAAHAALGCS
                     SWSRSDFILGPDGPVWLEVNTLPGLTPTSLFPQAAAYRGIDYSKLIHLLILDALVRRG
                     HSTPGSDAASSTASGKKKA"
     gene            1542..2381
                     /gene="dapF"
                     /locus_tag="PKN92_12535"
     CDS             1542..2381
                     /gene="dapF"
                     /locus_tag="PKN92_12535"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNZ49662.1"
                     /translation="MPFTKMHGLGNDYLYINTEISPVEDPAALSRAMSHRHLGAGADG
                     IILVMPGDNNPFRMRIFNADGSEAETCGNGIRCFAKYVYERNMTPEKEFVIETLAGPN
                     RVQLSVEDGKVLSVRSNMGSPRFDRAEIPMTGAPGKVLEEPIRLADGREFRVTCANIG
                     NPHAVFFVDDATAVDLTDLGPLVENHPSFPARTNVEFVTVLDRNNIVMRIWERGSGIT
                     MASGSGSCGAALASMITGRVDRKVQVHLVHGALEIHWSEEDNCVYQQGPATEVYEGIW
                     PLP"
     gene            2516..2968
                     /locus_tag="PKN92_12540"
     CDS             2516..2968
                     /locus_tag="PKN92_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49663.1"
                     /translation="MKTWKLASVFVIAMLVFAGTFSAEAAEKGISVTVTGECVNLLKA
                     VGGENAPEADGTFGALNALQVKEITDADGKAVEGVAGKLLHFLPTASASSLLGGEAGK
                     TVTVKGLLFKDAATLQVNEVGGGADAGGDFGDDWDEIGITTMSQQAII"
     gene            complement(3060..>3741)
                     /locus_tag="PKN92_12545"
     CDS             complement(3060..>3741)
                     /locus_tag="PKN92_12545"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387596.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HNZ49664.1"
                     /translation="NPNHYLAVAIDYLLTHRSRWSPHAAVGKTLVSSSLIDRVVARAG
                     RSLCEVPVGFKWFSPGLFDGSLCFGGEESAGASFLRRDGSVWTTDKDGLILGLLAAEI
                     TAVTGKDPGIYFNELTDSLGMPYFIRLDAPATAGEKSRLKALSPEDVQASELAGEAIV
                     SKLTRAPGNGEPLGGLKVSTENGWFAARPSGTEDLYKIYAESFLSDAHLAVLVSEAQQ
                     IVSAALHG"
     assembly_gap    3742..3751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3752..4720)
                     /locus_tag="PKN92_12550"
     CDS             complement(<3752..4720)
                     /locus_tag="PKN92_12550"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387596.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HNZ49665.1"
                     /translation="MSKNDAGASSPLAGKAVPRNLLIDTDALAKHYYGIFPELSDPAQ
                     RVSFGTSGHRGSSLKGSFTEAHVLAIAQAICDYRRLMHLDGPVFVGMDTHLLSALAQE
                     TVLEVLASNSMDVKVQAGGGVTPTPVISHAILSFNKGRQEHFADGIIITPSHNPPEDG
                     GIKYNPPTGGPADEAATGWIQDRANVLLEGGNQEVRRASLSGKAPSATDMITPYVEDL
                     RQVVNMERIAEAGLRLAVDPLGGSSLPYWEPINAVYGLDIDIVNPVLDNTFSFMTLDH
                     DGKIRMDCSSPWAMARLVELKERYDVAFASDPDADRHGIVTPSRGLL"
BASE COUNT         1191 a         1288 c         1321 g         1021 t
ORIGIN      
        1 ctttcgccac tttttcagaa cgccaaaatc tgatgaatgg cgctttcact ccatataaag
       61 cagttaacaa aataaacagg tatcttgttt ccttgttaac aagcacgccg gtctatacct
      121 gcaaaattaa ccgtatttca ttatgcacag caacatttgg cgcagccggt gtgcggtagc
      181 tgcccgcgcc atacagttgc tcagcaatca gagtatatta tagtaaaatc agcataagaa
      241 cgcaatacaa gtaagaaaag tgaccgatcc agaggaaagt gtgagtcaaa agtacagccg
      301 aagaaaaacg ctcctgcgcc gatccgaatc acaacggcaa cttgacaaat ccgggggcaa
      361 aaagcgtact atatagcctg cccgccggaa atttccggtg cgcatccccg tcttcctcct
      421 cttcaaaccc ggacgctgac catacagcct cccattcgaa aggcaaaaca tgtcggacac
      481 accctctttg cgtattgctg tcctcaaggg cggcatgagc ctggagcatg agatttctct
      541 tttgtccgca caaggtgttg aggcggctct ggaaagcctc gggtatgata tcgtaccggt
      601 gctgattacc agaacaggtg aatggcaggt ggaaggaagg aaaaaagagc cggcactgga
      661 cggcctcaaa aggctgaaaa agagcggtgt agactgcgtc tttcttgcat tgcacggagc
      721 aaatggagaa gacggccgca ttcaggggtt gctggatctg ctgaacatgc cgtatacggg
      781 atcgggctgc agcgccagtg cgcttgccat ggacaagccg ctggcaaaaa cagtggttgc
      841 cgctgccggt gtacaggtgg caccgcatct ctgctttact ccgtccctgt ggcagaaaga
      901 aaagcggaaa cttccgacca taagcaaagc cttgagtttc cccatggttg tcaaggcgcc
      961 ctgtcaggga tccagctgcg gacttgccat tgttgccaat gaaaaagagc tcgctcaggc
     1021 cattgaagag attatgccta tggaacagcg ggtcatggta gaaaactttg tgaaagggcg
     1081 ggagttcacc tgcgctgtgc tggatctgat cggcagcaat gcgatggaag cgctggcagt
     1141 gacagaaatc cggccgatta catccgcctt ttttgattat catgccaagt atacacccgg
     1201 cgcaacggaa gaaattaccc cggctcctct ccgtgcggcg gtgacaaaag caataggtgt
     1261 catggcgtgc gctgcacacg cggcattggg ctgcagctcc tggagtcgca gcgatttcat
     1321 cctcggaccg gatggacctg tctggctgga agtgaatacg cttcccgggc ttaccccgac
     1381 ctcgctcttc ccgcaggctg ccgcatatcg cgggattgac tacagcaaac tgattcacct
     1441 gctgattctg gatgcactgg tcagacgcgg gcacagcaca cccggctccg acgcagcttc
     1501 gagcacagct tcgggaaaaa agaaagcata agactatgcc gattcctttc acaaaaatgc
     1561 atggactggg caatgattat ctgtatatca acacagagat cagccctgtt gaagatccgg
     1621 ctgcgctgtc ccgcgccatg agccatcgtc atctgggcgc cggtgcagac ggcatcattc
     1681 tggtaatgcc gggcgataat aatccgtttc gcatgcgtat tttcaatgcc gacggaagtg
     1741 aagcagaaac ctgcggcaat ggcatccgct gctttgcaaa atacgtgtac gaaagaaata
     1801 tgacgcccga aaaagagttt gttattgaaa ccctggcagg ccccaaccgc gtccaactgt
     1861 cagtggaaga cggaaaagtg ctttccgtcc gttccaatat gggcagcccc cgtttcgatc
     1921 gtgctgagat ccctatgacg ggtgcgccgg gaaaagtgct cgaagagccg attcgccttg
     1981 ctgacggaag agagttccgg gtgacttgtg cgaatatcgg caacccccat gccgtctttt
     2041 tcgtggatga tgcaacagca gtcgatttga ctgatctggg gcctctggtg gagaatcatc
     2101 cgtcatttcc ggcgcgcacc aatgtggaat ttgtgacggt gctggacagg aataatattg
     2161 tcatgcgcat ctgggagcgc ggcagcggca tcaccatggc gagcggcagc ggctcctgcg
     2221 gtgcggcgct ggcgtccatg attaccggca gggtggatcg taaagtgcag gtgcatcttg
     2281 tccatggcgc cctggaaatc cactggtcgg aagaagacaa ctgcgtttat cagcagggcc
     2341 ccgccacaga agtgtacgaa ggtatctggc ctcttccctg aggaagagtt gcgttttttt
     2401 cggcggagac ggaaccgctc catgaatagg ggcttccgct tttcgtcttg ttattgtaac
     2461 aattggtaca ggccccggaa cggggtttca aacaacaggt gaaaggaaag aattgatgaa
     2521 aacctggaaa cttgcaagtg tgttcgtaat tgcgatgttg gtatttgccg gtactttcag
     2581 cgctgaagct gcggaaaaag ggatcagtgt taccgtgacc ggtgaatgcg tgaacctgct
     2641 taaagcggtt ggcggtgaaa atgcccctga agcggacggc accttcggtg cgctgaatgc
     2701 cctgcaggta aaagaaatca cggatgccga cggaaaggcc gtggaaggcg ttgccggaaa
     2761 actgctccac ttcctgccca cagcatctgc ttcctctctt ttgggcggtg aagctggaaa
     2821 aacagttact gtaaaaggac tgctgttcaa agatgcggca acccttcagg tcaatgaagt
     2881 cggaggcggt gccgatgcgg gcggcgactt tggcgatgat tgggatgaaa tcggcatcac
     2941 caccatgagc cagcaggcca tcatctgatc gcgctgcaac tttattttcc ggactgacaa
     3001 taaatcccct gcctgaaaca gtaatcgggc aggggatttt taatggcggc tgcggctcat
     3061 cagccatgaa gagcggcgga cacaatctgc tgcgcttcgg ataccagcac cgcaagatgg
     3121 gcgtcactca ggaaactttc agcataaatt ttataaagat cttccgtccc cgacggacgg
     3181 gcggcaaacc agccgttttc cgttgaaact ttaagaccgc ccagaggctc tccattgccc
     3241 ggcgcccggg tcaacttgct cacaatcgct tcgccggcaa gttctgacgc ctgaacgtct
     3301 tccggagaca gggctttcaa ccggcttttt tctccggcag tggcgggcgc atcaaggcgg
     3361 ataaaataag gcatgccgag gctgtcggtc agctcattga aatagatgcc cgggtctttg
     3421 cctgtgacgg cggtaatctc agcggcgaga agcccgagaa tcagcccgtc tttgtctgtt
     3481 gtccagacag agccatcgcg ccgaagaaaa ctggcgcccg cactttcttc gccgccaaag
     3541 caaagggaac cgtcaaacag cccgggagaa aaccacttga accccacggg cacctcacac
     3601 agagaacgcc ctgcccgcgc cacaactcga tcaatgaggc tgctgctcac cagtgttttg
     3661 cctacagcag cgtgcgggct ccatctggag cggtgggtaa gaagataatc aatagcaacg
     3721 gcgagataat ggttggggtt cnnnnnnnnn ncagcaatcc ccgggaagga gtgacaatgc
     3781 cgtgccggtc ggcgtccgga tcactggcga aagcgacatc gtaacgctct ttcagttcta
     3841 caagccgcgc catcgcccag gggctggagc agtccatacg gattttgccg tcatgatcaa
     3901 gagtcatgaa agaaaaggtg ttgtccagaa caggattgac aatgtcgata tcaagaccat
     3961 acacagcatt gataggctcc cagtaaggca aggaagaacc gcccagcgga tcaaccgcaa
     4021 gacggagtcc cgcctccgca atccgttcca tattcaccac ctgccgcaaa tcttccacat
     4081 agggcgtgat catgtcggtt gcagagggcg ctttgcccga cagagacgca cggcgcactt
     4141 cctgattccc gccttccagc agaacattgg cgcgatcctg aatccagccg gtcgccgcct
     4201 catcagccgg accgcccgtt ggagggttat atttgattcc gccgtcttca ggcggattat
     4261 gggagggagt aatgatgatg ccgtccgcaa aatgctcctg cctgcctttg ttgaaagaaa
     4321 gaatggcgtg agaaataacg ggcgtaggcg tcacgccccc gcctgcctgc accttaacat
     4381 ccatgctgtt gctggcaagg acttccagca ctgtttcctg tgcaagtgct gacagaagat
     4441 gggtatccat cccgacaaac acagggccgt ccagatgcat aagccgcctg tagtcgcaga
     4501 tagcctgtgc aatcgccagc acatgcgctt cggtaaaaga gcctttcagt gaagaacctc
     4561 tgtgcccgct ggtaccgaaa gaaacacgct gcgccggatc ggaaagttcg gggaaaatac
     4621 cataatagtg ctttgcaagt gcatcggtat caatcagcaa attacggggc accgccttac
     4681 ctgccagagg tgaggatgcg ccggcatcat tcttactcat agtatctcct gtactcgcct
     4741 gcccggggaa cgctgcaatc aaaatgcgcc tcacacggaa gaaacgccac aactttcatt
     4801 gtacgatata ataacacgtt ccgccagaaa g
//
LOCUS       DAOPWB010000194         4733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_18169_length_4733_cov_4.918982, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000194 DAOPWB010000000
VERSION     DAOPWB010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18169_length_4733_cov_4.918982"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(379..2382)
                     /gene="uvrB"
                     /locus_tag="PKN92_12555"
     CDS             complement(379..2382)
                     /gene="uvrB"
                     /locus_tag="PKN92_12555"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNZ49666.1"
                     /translation="MDAAPFELISDLAPAGDQPEAIEALVQGVEEGLPHQTLLGVTGS
                     GKTFTIAHVIARTCRPTLVLAHNKILAAQLYGEFKALFPNNAVHYFVSYYDYYQPEAY
                     IPTTDTYIAKDSTINDEIDKMRHAATRAVLTRRDCIVVASVSCIYGIGTPQAYMGLRV
                     SLETGMAPGRDKVVDDLISMQYARNDFDFHRGVFRVRGDIVDVFPAYEADRAVRIEFF
                     GDEIEGLSEIDPLRGSVIRKLRRAEIFPGSHYVAEKGTMERALVSIRLELQDRLKELR
                     EEKQELFAQRLDQRTRYDLEMLEELGFCTGIENYSRHLDGRQPGEPPYTLLSYFPEDF
                     LLVIDESHMTIPQVGAMFRGDRSRKDKLVEYGFRLPCARDNRPLTFEEFEERLNQVIY
                     VSATPASYELEKSEGLIIEQVVRPTGLVDPEVEVRPVSDQVDDLLGEIRIRAEKGERC
                     LVTTLTKRMAEDLTEYYRDLGVRIRYMHSDVETLERIELIRQLRQGQYDVLVGINLLR
                     EGLDIPEVSLVAILDADKEGYLRSATSLIQTCGRAARNIAGRVIMYADKMTDAMKQAL
                     DETGRRRKKQLAYNKKHGITARSIRKAIPDTVAILTERDYLDLSKAAETPEIYVSERD
                     FNKSITRLRKEMKAAADKMDFEHAALCRDRIQELEIKRIEMGL"
     gene            complement(2386..4512)
                     /locus_tag="PKN92_12560"
     CDS             complement(2386..4512)
                     /locus_tag="PKN92_12560"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNZ49667.1"
                     /translation="MHEAFAKLLRILNKKCSTEQLEVVRHAYRFANEAHQGQTRASGV
                     PYIMHGLEVATILAENDQDYITCAAGLLHDVVEDTHHPVERIRQEFGEEIAGLVEGMT
                     KISSLSFSEGMLSREEQQAQNIRKMLIATIKDLRVLFLKLADRLHNMRTLEYLKPEAQ
                     QRIAQETLTIYAPLANRMGVSEWKWELEDRSFRYLMPGSYREISRQVNMRREERKKYI
                     QETIETLEPHLLAKGITARMTGRPKHLYSIYQKMTSQAKEFSEVHDMHGIRIITRTVT
                     ECYTALGVVHEMWQPDPALPFKDYIARPKINMYQSLHTTVLREMGRKMEVQIRTEEMD
                     RIAREGIAAHWQYKEGARNPDDSLTARLTWLRKMYDWLQDDEGSDNLMESMVHDLATP
                     YIYVVTPRGEVKELPEGATPLDFAYMIHSQIGHHCTGARVNNKFVPIRYNLQTGDCVE
                     ILTSKNQEPHISWLDVVTTGRARSRIRQRLREIGALPPAEDKHSEPRSRRPHFTPHVT
                     PQPLIREVDEATRRQMLRVDGKSGIQVQFAKCCDPMPGHEIIGYVTLASGISIHRTDC
                     GNIARPSRDTSRLIQVSWESEAPVIAQMRLVTRSRPNLLADITNALRPLNIEILNAHF
                     GPGSQENNHFDFQYEAREKAVMDQVAYAVGQVPGVFRVMHMGVRQKEKRNRAPRSEKK
                     PVSREEKKEVPRRHHPRKKMTRHGEA"
BASE COUNT          964 a         1393 c         1228 g         1148 t
ORIGIN      
        1 attggcacac aagcgggtca tgatgaagac agtcgctcag attcgccgcg gaaataggtt
       61 ctgagacata ttcccataat cacagaccac acacgcttct gccaatacgg agattggcgt
      121 tcccaggctg gcggatagaa tgttttaagt cagagtatta tggtgtgaag ctcacataag
      181 tctcaggtag aaattcgtgt cccttcgtgg ttcttcccca tgccaatagg tcgattggcg
      241 ctctcagaag atgcggcgac ggcttttata agggtggggt catgcaccga acgcacagtc
      301 cactttgtcc gcggtttttg gtgtggtgcg cagcttttga atcagcagcg ttcttgcgtc
      361 cttgtccggg ctggaggatt ataaacccat ctctatgcgc tttatttcca gctcctgaat
      421 gcggtcccgg cacagtgccg catgttcaaa atccatttta tccgccgccg ctttcatctc
      481 tttgcgcagc cgggtaatgg atttattaaa gtcccgctcc gaaacataga tttccggcgt
      541 ttccgccgct tttgacagat ccagatagtc ccgctccgta agaattgcca ccgtatccgg
      601 aatcgctttt cggatggagc gtgctgtgat gccatgcttt ttgttgtaag ccagctgctt
      661 tttccgccgc cgccccgtct cgtccaatgc ctgtttcatg gcgtcggtca ttttgtcggc
      721 atacatgatc acccgcccgg caatatttcg cgccgctctg ccgcaggtct gaatcaggct
      781 tgttgccgaa cggagatagc cttccttatc ggcatcgaga atggcaacga gagacacctc
      841 gggaatgtcc agcccctcac ggagcagatt gatgcccacc agcacgtcat attgtccctg
      901 gcgcagctgc cggatcagct ctattctttc gagagtctcc acgtcagaat gcatatagcg
      961 gattcgcacc cccagatccc tgtagtattc cgtaagatcc tccgccatac gcttggtcag
     1021 ggtcgtcacc agacaccgct cgcctttttc cgcccggatc cggatctcgc cgagaagatc
     1081 gtccacctga tcgctgaccg ggcgcacctc cacctcggga tccacgagcc ccgtcgggcg
     1141 caccacttgc tcgatgataa gcccttcgct tttttccagt tcataagatg cgggcgtggc
     1201 acttacataa ataacctggt tcagccgctc ttcaaattct tcaaaagtaa gaggccggtt
     1261 gtcccgggca cagggcaggc ggaatccgta ttccaccagt ttgtctttgc gcgaccggtc
     1321 tccccggaac atggcgccca cctgaggaat cgtcatatgg ctttcatcga tgaccagcag
     1381 aaaatcctcg gggaaataac tgagcagcgt atagggcggt tctccgggct gcctgccgtc
     1441 gagatggcgc gaataatttt caatccccgt acaaaacccc agttcttcca gcatttccag
     1501 atcgtagcgt gtacgctgat cgaggcgctg cgcgaagagc tcctgctttt cttcccgcag
     1561 ttctttcagg cgatcctgca gctccagccg gatggaaacc agcgcccttt ccatcgtgcc
     1621 tttttccgcc acataatgag agcccgggaa gatttccgcc cgccgaagtt tccggatcac
     1681 tgaaccgcga aggggatcta tttccgaaag tccttcgatc tcgtcgccga aaaattcgat
     1741 acgcaccgca cgatccgctt cataggcggg aaacacatcc acaatatcgc cgcggaccct
     1801 gaataccccg cggtgaaaat caaagtcatt tcgggcgtac tgcatgctga tcaggtcgtc
     1861 caccacttta tcacgcccgg gcgccattcc cgtttccagc gacacccgaa gccccatgta
     1921 ggcctgaggc gtaccgattc cgtaaatgca ggatacgctt gcaacaacaa tgcaatcgcg
     1981 ccgggtcagc accgcccggg tcgcagcatg ccgcatcttg tcgatttcgt cattgattgt
     2041 cgagtccttg gcgatatagg tgtccgtggt gggaatatag gcttccggct gataataatc
     2101 ataatagctc acaaaatagt ggacggcatt gttcggaaag agcgccttga attcaccgta
     2161 gagctgggcg gcaagaattt tgttgtgcgc cagtaccagg gtgggacggc aggtgcgtgc
     2221 aatgacatgg gcgatggtga atgtttttcc tgagccggtg actccgagaa gggtctggtg
     2281 agggagccct tcctccacgc cctggacaag cgcctctatc gcctccggct gatcgccggc
     2341 aggagccaaa tcactgatga gttcaaaggg tgccgcatcc aaaactcagg cctcgccatg
     2401 tcgcgtcatt ttttttcggg gatgatgccg acggggcacc tcttttttct cttctcggga
     2461 caccggtttt ttttcggacc ggggcgctct gtttcgtttt tctttctgac gcacacccat
     2521 gtgcatcacc cggaacacgc ctggcacctg tcccacggca taggcgacct gatccatcac
     2581 cgccttttcc cgggcttcat actgaaaatc gaagtggttg ttttcctgac tgcccggacc
     2641 gaaatgggca ttcagtatct cgatattcag cgggcgcagc gcattggtaa tatcggcaag
     2701 caaattgggc cggctccggg tcaccagacg catctgggca atgaccggtg cctcgctctc
     2761 ccaggaaacc tgaatgagcc gggaggtgtc ccgggaaggc cgggcgatgt tgccgcaatc
     2821 tgtacggtga atcgaaatac cggaggcaag ggtcacataa ccgataatct catgaccggg
     2881 catgggatcg cagcatttgg caaactgtac ctgaatcccc gactttccgt cgacccgaag
     2941 catctggcgg cgtgtcgcct catccacttc gcggatcagc ggctgcggtg tcacatgggg
     3001 cgtaaaatgg ggacgtctgc tccggggttc actgtgctta tcttcggcag gcggtaaggc
     3061 gccgatttcg cgaaggcgct gcctgatccg ggaccgggca cgtcctgttg tcaccacatc
     3121 cagccagctg atatggggct cctgattttt ggaagtcaga atttccacac aatcgccggt
     3181 ctggaggttg tagcgtatgg gtacaaactt gttgtttacc cgtgcgcccg tgcaatgatg
     3241 cccgatctga gaatggatca tataggcgaa gtccagaggc gtggcgcctt ccggcagttc
     3301 cttcacttca ccccggggcg ttaccacata aatgtagggt gtcgccaggt cgtgaaccat
     3361 gctctccatc agattatcgg agccttcatc gtcctgaagc cagtcgtaca tttttcgcag
     3421 ccacgtgagc cgcgccgtca gcgaatcatc cgggttacgt gccccttcct tgtactgcca
     3481 gtgtgcggca atcccttccc gggcgatacg gtccatctcc tccgtccgga tctgcacttc
     3541 cattttcctg cccatctcac ggagcaccgt cgtgtgcaga gactggtaca tattgatctt
     3601 agggcgggcg atatagtctt taaacggcag ggcgggatca ggctgccaca tctcatgcac
     3661 cacgcccagt gccgtgtagc actcggtcac cgtccgggtg ataatccgga ttccgtgcat
     3721 gtcgtgcacc tcgctgaact ctttcgcctg actcgtcatc ttctgataaa tgctgtaaag
     3781 gtgcttgggc ctgcctgtca tgcgcgccgt aatccccttc gcaagaagat gaggttccag
     3841 cgtctctatc gtttcctgaa tatacttctt tctttcttcc cggcgcatgt tcacctgacg
     3901 ggaaatttca cggtaggagc cgggcatcag ataacggaag gaacggtctt ccagttccca
     3961 tttccattcc gacacaccca tgcgattggc gagaggtgcg taaatggtta acgtctcctg
     4021 cgcaatgcgc tgctgcgcct caggcttcag atattccagc gtgcgcatat tgtgcagccg
     4081 gtccgccagc ttgagaaaaa ggacacgcag atccttgatc gtggcaatca gcatcttgcg
     4141 gatattctgc gcctgctgtt cctcgcggct gagcatgcct tctgaaaaac tcagactgct
     4201 gatcttggtc atgccctcca ccagaccggc tatttcttcg ccgaattcct gacggatacg
     4261 ctccactgga tgatgcgtgt cctccaccac atcatgcaga agccccgccg cacaggtgat
     4321 gtagtcctgg tcgttctccg caagaatcgt cgccacttcc agtccatgca tgatataggg
     4381 aacccctgag gcacgcgtct gaccctgatg cgcttcattg gcaaaacggt aggcatggcg
     4441 caccacctcc agctgctcag tggagcactt cttattcagg attctcagca gttttgcaaa
     4501 ggcctcgtgc atgggtcgtc cctgtcagag cagttgagga aatcctcctg cgttctgata
     4561 tacaagagta taccactttg aaggtctccg tcggatatgt ccccacctgc gaccggatga
     4621 ctgggcactt ttttactacc tgagacttat ttgcccccga agcaacataa tccgccagcc
     4681 ctgcacaccc cgtcatccct gcctttccac cgtcattcct gcgaaggcag gaa
//
LOCUS       DAOPWB010000195         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_18291_length_4710_cov_4.143502, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000195 DAOPWB010000000
VERSION     DAOPWB010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18291_length_4710_cov_4.143502"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2188)
                     /locus_tag="PKN92_12565"
     CDS             complement(<1..2188)
                     /locus_tag="PKN92_12565"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNZ49668.1"
                     /translation="MLRKWMNTCVLTVLVLGLTAGVAVGQTCIYKDDLGNPKMCVAGA
                     AIADALASVDTGGKFAQFHNFSTWDLNEDGVSDNDQLMLLTELLCLPDGAKHPFIDIQ
                     AVKDTFDANLVVYQDLMNKAEANLAAASALAPLAKSLGKQMLDSGKPELNQPIEDAAF
                     LSAAVPANWAGKKYSALANMIYKAGDGYVWHESKGNIETMRLALTSDGAAFLGAAVLG
                     LGDDTYVDRVLGSVWGDRALRALALLDLDWAYLEALFALDAGEVTDIQDAVDTVTVDE
                     ADVPEYGVDFETLEEIQADSVWDEGGTTLADMLADASGDEVIVADEMEAMVIYPVYMP
                     NVVGEKFEDALAAISALGLIVTDGVPTFSDTVDPGYVISQTVAPGTMLYPGDGTVLFV
                     SVGENACPEFFVTPETTIFDVGGAAIADAIAFIDVNGDYPQYHTYAAWDLNEDGVADK
                     AQFRLLAHLLCLPPQYLHPTIDLAAVRDVFALNLNTYQELRALAVANIDIAKNLAQPS
                     IDVANLMRLPANAAKIVELIEDDAFLTKGTVPAEWAGLRYSDLANFLFKAGDGTKYHD
                     SHGNFTAAQNAFDSDAAAYLGAAAFALGDGSIVDDVLNSSWGKRVLRGLVLKNLDWDY
                     LAVKFGLPDALRDAIKSEVAGSTVDVDSIPDWGIDLDAVAALDPGAIIDGGKTLAELL
                     ADLGGDIDAIFDLVEEDLNLPAEGEVEGEVEGEVEGEVEGEVEGEVE"
     gene            complement(2363..4588)
                     /locus_tag="PKN92_12570"
     CDS             complement(2363..4588)
                     /locus_tag="PKN92_12570"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HNZ49669.1"
                     /translation="MRNVQFVALFTCVLTGLVFSASAQDCEFRNEFGESNLNGGGEAV
                     AAAMAMLPSMGINNVPAMFQGPYATWDINSDGIPDKDQFELLTTLLCLPEGVQHPLLD
                     VAAMKEGFDANLALYWNLVDVVFDNEADIVALAPQAKAVGHQLRIAVNSRGYRNRPLD
                     PGVFLDQVIPELWEGKTYGELADTLYELGADIEYLILKGLYGSPTSLLRTTLVNDLTA
                     AFVAAAMGPLLTELFGVASFSSLQSGLLLKTLDWDFLSDLFALYGLNAGQAAVFGDAF
                     SALDVPDDFVPDPDEDVSYYEWNGIFYDDVYLLGDIEDLIASVLDDLLPFTMPVRVVH
                     ASPDLEAIAICQDAATFVGDLAFKDISGYEAASAWQPDFQYEFGLIHQGGDCLLPLLS
                     DSFDLDNTSTLVVLNRAINPEFLMVGDDLEMSRTGHARIRFLNASPDAEPLTLTGQVN
                     GAAAAPLFSDIAFKNVTGYTEVVPGIYTLGFTTGDKSNKALPQEQIVLNAGSVYTVFA
                     MGLAENMEMAVNADTEPGTVTVTADPVRTSFFAGESVQFTAVCSPELTPVSYAWLRNG
                     VALPGETGAELSIPSLAAADGGSYTVIVEAGVANVVGRVQVSASAPAIQVIKPVVSIV
                     GRRELASGEDLRLNAVVSGGATVLSYNWTWADGPLPGISTTDPQLVILSVDSERRGSY
                     RVEVEVALPGYPSVRKLVAAEVYVRIDLVPMPAASLIALGLLFAVLFVAGGMRVQKKV
                     S"
BASE COUNT         1232 a         1357 c         1126 g          995 t
ORIGIN      
        1 cctcgacttc gccttcaact tcgccttcaa cttcaccctc aacttcgcct tcaacttcac
       61 cctcaacttc gccttcagcg ggaagattga ggtcttcttc aaccaggtca aaaattgcgt
      121 ctatatcgcc accgaggtct gcaagcagtt cggcaagtgt ttttccgccg tcaataatcg
      181 caccaggatc aagggctgca acagcatcga ggtcaattcc ccaatcgggg atgctgtcga
      241 catcaacagt agaaccggcg acctcgcttt taatggcatc acgcagcgca tcgggaagac
      301 cgaatttcac cgcgaggtaa tcccagtcca gatttttcag cacaagcccg cgcagaacgc
      361 gcttacccca ggaactgttg agaacatcgt caacaatgga gccgtcgccc agtgcgaaag
      421 cggccgcacc caggtacgct gcagcgtcac tgtcaaaggc gttttgtgct gctgtaaagt
      481 ttccgtgcga atcgtggtat ttggtaccat cgccggcttt gaaaaggaaa tttgccaggt
      541 cgctataccg cagccctgcc cattcggccg gaaccgttcc tttggtcagg aatgcatcat
      601 cttcaatcag ctcaacgatt tttgcagcat tagcaggcag gcgcatcaaa ttcgcaacgt
      661 caatggacgg ctgtgccagg tttttggcga tatcaatatt cgccaccgcc agagcacgca
      721 gctcctgata ggtgttcaaa ttaagcgcaa agacatcacg caccgcagcc agatcaatgg
      781 taggatgcag atactgcggc ggaaggcaaa gcaggtgcgc cagcaggcgg aactgcgctt
      841 tgtcagcaac accgtcttca ttcagatccc aggcggcata ggtatgatat tgcggatagt
      901 caccattaac gtcgataaaa gcaatagcgt cggcaatagc ggcaccgccc acgtcaaaaa
      961 tggtggtttc gggggtgaca aaaaattcag ggcaggcgtt ttccccgacg gacacaaaaa
     1021 gaaccgtgcc gtcaccagga taaagcatag taccaggcgc cactgtctgg gaaatgacat
     1081 agcccggatc tacggtatca ctgaaagtgg gaacaccgtc ggtcacgata agaccgagcg
     1141 cactgatggc agccagcgca tcttcaaatt tctcgcccac tacattgggc atataaacag
     1201 gataaataac catcgcctcc atctcgtcgg caacaatcac ttcatccccg ctggcatcgg
     1261 caagcatatc cgcaagcgtt gtaccgcctt cgtcccagac ggaatcggct tgaatttctt
     1321 caagcgtttc aaaatcaacg ccgtattcag gaacatcggc ttcgtcaacg gtaaccgtat
     1381 cgacggcatc ctgtatatcc gtaacttcac ccgcatccag agcaaaaagg gcttccagat
     1441 acgcccagtc cagatcgagg agcgccagtg cacgcagggc gcgatcaccc cacacggagc
     1501 caaggactct gtctacatag gtatcatcac ccagtccgag aacggcagcg cccaggaaag
     1561 cagcaccgtc ggaggtgaga gcgaggcgca tggtctcaat attgcccttg ctctcgtgcc
     1621 acacatagcc atcgcctgcc ttgtaaatca tgtttgccag ggcgctgtat tttttacccg
     1681 cccaattggc aggaacagca gcactcaaaa aggcagcgtc ctctatcggc tgattcaatt
     1741 ccggctttcc ggaatccaac atctgtttgc caagcgactt ggcaagaggt gccagagcag
     1801 atgcagcagc cagattcgct tccgccttgt tcatcaaatc ctgataaacg acaagattgg
     1861 catcaaaggt gtcttttacc gcctgaatat caatgaaggg atgcttggcg ccatcaggca
     1921 ggcacagcaa ttcggtgagc agcatcagct gatcgttatc gctcacaccg tcttcattca
     1981 ggtcccaggt actgaaattg tgaaactggg cgaactttcc gcctgtgtca acgctcgcaa
     2041 gtgcatcagc gatagcagca ccggcaacgc acatcttcgg attgcccagg tcgtccttgt
     2101 agatgcaggt ctgaccaacg gcaacacctg ccgtcaggcc taagacaagc actgttaaaa
     2161 cgcacgtatt catccatttc cgcaacataa atgtttctcc ttgtttcttc gttcgtctgg
     2221 gtcgggtgcc agtctgcccc gcaacccacc cacggtcagt ggaacgtttt cacttcctac
     2281 accagttaac tacaatacct taccacttaa ttattgtaac tttagcacaa acaacctgcg
     2341 tatgtcaact atttctcgcc ttttaagaaa cttttttctg aacccgcatt cctcctgcaa
     2401 caaaaagcac tgcaaataac aaacccagcg ctataaggct tgcggcaggc atgggaacaa
     2461 gatcaatacg gacataaacc tccgcagcga ccagtttacg gacagaggga taaccgggga
     2521 gagcgacttc cacttccaca cgataggagc cgcgccgctc cgagtccaca ctcagaataa
     2581 ccagctgagg atccgttgtg ctgatgcccg gcagcggacc atccgcccag gtccagttgt
     2641 aggaaagcac cgtggcaccg cccgaaacga ccgcattcag acgcaggtct tcgcctgagg
     2701 caagctcgcg gcggccgaca atggacacaa ccggcttaat cacctgaatg gcaggggcgg
     2761 aagctgaaac ctggacacgt ccgacaacat tggcgacccc cgcctcgact atgaccgtat
     2821 aactgccgcc gtcggctgct gccagactcg gtatgctcaa ttcggcaccg gtttcaccag
     2881 gcagggctac gccgtttctg agccaggcat aagagacagg cgtcagttca gggctgcaaa
     2941 cggcagtgaa ctgaacgctt tccccggcaa agaagctcgt gcgcaccgga tcagcggtga
     3001 cggtcacagt tccgggctca gtatcggcgt taaccgccat ctccatattt tctgccagcc
     3061 ccatggcaaa cacggtgtag accgacccgg cgtttagcac gatttgctct tgaggaagtg
     3121 ctttgttgct cttatcgccg gttgtgaaac ccagcgtgta aatgccgggc accacctccg
     3181 tgtagccggt aacattttta aaggctatgt cagagaaaag tggagccgcc gcagcaccat
     3241 tcacctgccc ggttaaggta agcggctctg catcggggct tgcgttcagg aagcggatgc
     3301 gtgcgtggcc tgtccgggac atctcgagat catccccgac catcagaaac tcagggttga
     3361 tcgcccgatt cagcaccacc agcgtgctgg tattgtccag atcaaaactg tcggacaaaa
     3421 gcggaagcag gcagtcaccg ccctgatgaa tcagaccgaa ttcatattga aaatcaggct
     3481 gccaggcaga ggcggcttcg tagccggaaa tatctttgaa ggcaagatct ccaacgaaag
     3541 tagcagcgtc ctggcaaata gcgatcgctt ccagatccgg gctggcatga acgactctga
     3601 ccggcatggt aaagggcagc aaatcatcca gcactgaggc aatgagatct tcaatatcgc
     3661 ccagcaggta cacgtcatca taaaagatgc cgttccattc gtagtacgaa acatcctcat
     3721 ccggatccgg gacgaaatca tcagggacgt ccagggcgga aaaggcatcg ccaaagactg
     3781 ctgcctgccc cgcatttaac ccgtacaaag caaaaagatc actcagaaaa tcccagtcca
     3841 gagtcttcag aagaagtcct gattgcagag aactgaagct tgctacgcca aaaagctccg
     3901 tcagcaacgg ccccatcgct gctgccacaa aagccgctgt caaatcgttt acgagagtcg
     3961 tgcgcaaaag agatgtgggg cttccataaa gtcctttaag aatcagatac tctatatcgg
     4021 cacccagttc gtaaagcgtg tccgccaatt caccgtaggt tttcccttcc cacagttccg
     4081 ggataacctg atccagaaaa acgcccggat caagaggacg gttacgatac ccgcggctgt
     4141 tgaccgctat tcttaactga tgcccgacag ctttcgcctg aggcgccaaa gcaacgatat
     4201 cagcctcgtt gtcaaagaca acatcaacga gattccaata cagggcaaga ttggcatcaa
     4261 agccctcttt catggccgcc acatcaagga gcggatgctg cacaccttcg ggcaagcaaa
     4321 gaagcgttgt taaaagttca aattggtctt tatccggtat gccgtccgag ttgatatccc
     4381 aggtcgcata aggaccttga aacattgcag ggacattgtt gatgcccata ctgggaagca
     4441 ttgccatcgc cgcagcaacg gcttcccccc cgccattcag atttgactct ccgaactcat
     4501 tccggaattc gcaatcctgc gctgacgcag aaaaaaccaa tcctgtcaat acacaagtaa
     4561 acaacgcaac aaactggacg tttctcacca gactatcctc ctcgggatta ttatccactt
     4621 taggctacca aacagccgaa tcccatgctg ataggttttt aactcaatct tgtaagatta
     4681 taccataatc aattaaaatt tgcaagccaa
//
LOCUS       DAOPWB010000196         4704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_18314_length_4704_cov_4.993977, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000196 DAOPWB010000000
VERSION     DAOPWB010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18314_length_4704_cov_4.993977"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..931
                     /locus_tag="PKN92_12575"
     CDS             2..931
                     /locus_tag="PKN92_12575"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNZ49670.1"
                     /translation="MRELNRSEIECILDTAPQFLAVSQRASGIKKVPLLQGRLVVNWF
                     HEPSTRTRTSFELAAKRLSADTLSMTASSSSHVKGETLHDTLANIEAMRSDIIVLRHS
                     CAGAPHMLAADHSSHIINAGDGRHEHPTQALLDAFTMREARRKISGNPVATLDGAKVA
                     LIGDVEHSRVARSNIFALTRLGAHVTVCGPVALLPSQVEKLGVHSTTRLEEALSDADF
                     IYALRIQKERQGSGLFPSIREYVKLFRIDRDSLRFAPDHALVLHPGPINRDLELSTEV
                     ADGSRSLVLRQVTNGVAIRMAVMHLLANSRTRQ"
     gene            1002..2273
                     /locus_tag="PKN92_12580"
     CDS             1002..2273
                     /locus_tag="PKN92_12580"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNZ49671.1"
                     /translation="MSLVISNGLVIDPANNLSGICDVLIEDGKIKEISTALQGDKVLD
                     ATGLVVCPGLVDIHCHFREPGFESKETIASGSRSAARGGFTTVVTMANTNPPIDNAGL
                     VELVIRRAREEACIHVCPAACATKDLKGSEITEMADLKAAGAVAVTDDGRDISSSSVM
                     RHALDYAGMVGLPFLSHSEDEALTAGGAMNEGYCATLMGLPGIPKVAEDIRTDRNIRL
                     AEIANAHIHIQHVSTAASVEIIRQAKKRGAKVTAESAPHYFSLTDEATLNFNTNAKMN
                     PPLREKEDVEAIIEGFKDGTLDCIATDHAPHTQTEKRVEFEVAPFGIIGLETSLALTI
                     THLVNPGHVSLEKAVALLSPGGARVCNLNAGTLTPGVSADIAIFDPQEKWTVLSEEFA
                     SKSCNSPFVGATLQGRVRYTLCKGTVVYSAS"
     gene            2301..3416
                     /locus_tag="PKN92_12585"
     CDS             2301..3416
                     /locus_tag="PKN92_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49672.1"
                     /translation="MPEYQGFRFYKDESTMERALLEQGVILAAGWKRRAEYLSIHPRD
                     LTVLDKPQGITLSDSEFLDLQSARSEEACQWWEKQVDEYLQYWTARLKVFEEKQSRLL
                     NAVEKGRLTPARANQRAQKFSRQHEHARMQIQLCKDLAACLQGDHSLPLPVLPLCQYS
                     VSNHTHSRNWMSASSALKKSQSSILSRITGRFVAGSLSFHSLSITDRTALVLSLLLSA
                     LIAAGGCMYAFRGEKIAITVKAIDRGEYEVSFSNTSPKTLALILPYNGGGLEKSIPPP
                     LGVTVELLDKKKNLPLNQSIESLWYYRDKSAYLKGPVLLSPFSQEVLSLRLDRDEIEK
                     TSASEQLQIRLTIFRAPNRKSKSFTFPLSSDAGHPKE"
     gene            3649..>4704
                     /locus_tag="PKN92_12590"
     CDS             3649..>4704
                     /locus_tag="PKN92_12590"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ49673.1"
                     /translation="MKNTHCYARSLILFLALSISIYALAQGPVVSKVQVQPRTDGSGN
                     VDIYYDLHHPKGLASTASLRLSKDGGASDPFTLVHTSGHTGTNVTPGSNGHSLWEVGT
                     EFPGEEISRAVIEVAATGHNEEEGEIIPGEMITIPAGSFKMGRPYTDIGSPDELPVHT
                     VYLDRYQIGKYQVTNEEFAAVLNWAYARNLLKNSTGEAYTDGAIYANGQQLAETESSS
                     DFSQISFVNNRFSVRSRKGLDDRLCFMANHPVVMVSWYGAVCYCNWLSEKEGLESCYH
                     TTSWKRHEPVRNGYRLPTEAEWERAAAWDRTKHWRYGMTSDSIHVTKANYFPKRFANP
                     LGLKTDPYTSPVGWYNGV"
BASE COUNT         1184 a         1222 c         1180 g         1118 t
ORIGIN      
        1 catccgagaa ctgaaccgga gtgaaataga atgtatcctt gatacagcgc ctcaatttct
       61 cgctgtctct cagcgggcga gcggcattaa aaaagtacct cttctgcagg gaagactggt
      121 tgtaaactgg tttcatgagc ccagtacccg tacccgcacg tcttttgagc tcgcagcgaa
      181 acgcctgagt gcggacaccc tttccatgac agcctcctca tcaagccacg tcaaaggtga
      241 gacactccac gatacgctgg caaatatcga ggctatgcga tccgacatca ttgttttgcg
      301 tcacagctgc gccggtgccc cgcatatgct tgccgcagat cacagctcac atattatcaa
      361 cgccggagac ggccgccacg agcatcccac ccaggcgctt ctcgatgctt ttaccatgcg
      421 cgaagcacga cgcaaaataa gcggaaatcc ggttgcaact ctggacggtg caaaagtggc
      481 tctaatcggt gacgttgaac atagccgcgt agcgcgcagc aacatatttg ccttgacccg
      541 gctgggtgct catgtaacag tctgcggtcc tgttgctctt cttccgtcgc aggttgaaaa
      601 actgggtgtc cattcaacca cccgcctcga agaagcactg agcgatgcag actttattta
      661 tgcgcttcgt attcaaaaag aacgccaggg cagcggtcta ttcccaagta tccgtgaata
      721 tgtaaagctt ttccgcattg atcgggactc ccttcgtttt gcgcccgatc atgcattggt
      781 tcttcatccg ggtcccatca acagggatct ggagttgtca acagaagtag cagacggatc
      841 gcgcagtctg gtgcttcgtc aggtgaccaa cggtgtcgcc atccgaatgg cagtcatgca
      901 tctgctcgcc aacagcagaa cgagacagta aacgcctgtt gccgcttagc agcaagcact
      961 aacaaatgaa aattctctag tgtggtgtaa ggagacctta cgtgagtctt gttatttcaa
     1021 acggccttgt gattgatccg gcaaacaact tatcgggtat ctgcgacgtg ctgattgaag
     1081 acgggaagat taaggaaatc agcacagccc tgcaagggga taaagtgctg gatgccaccg
     1141 gtcttgtagt ctgtcccggt cttgtggata ttcattgcca tttccgtgaa cccggctttg
     1201 aatccaagga aaccattgct tccggcagcc gctctgctgc ccgcggagga ttcactacag
     1261 ttgtcactat ggcaaacacc aacccgccca ttgacaatgc cggattggta gagctggtca
     1321 tccgccgtgc ccgcgaagaa gcctgcatcc acgtatgccc tgccgcctgc gccacgaaag
     1381 atctgaaagg atcggaaatt acagaaatgg ctgatttaaa agcggcaggc gccgtagcgg
     1441 ttaccgatga cgggcgcgac atcagttctt catcggtgat gcgccatgcc ctggactatg
     1501 ctggaatggt cggactgcct tttttaagcc atagtgaaga cgaggccctg accgccgggg
     1561 gcgccatgaa tgagggatat tgtgcaacat taatggggct gcccgggatc ccgaaagtgg
     1621 cggaagacat acgcacggac agaaacatcc ggttggcaga aattgccaat gcacatattc
     1681 atatccaaca tgtttcgact gctgcgagcg tcgaaattat tcgacaggcc aaaaaacggg
     1741 gtgcaaaagt tacagcagaa tctgcgccgc attatttcag cctcaccgat gaagcaactc
     1801 tcaacttcaa caccaatgca aagatgaatc ctcctctccg ggaaaaagaa gacgtggaag
     1861 caatcatcga gggcttcaag gacggcaccc ttgattgtat tgccacggat catgccccgc
     1921 acacgcaaac cgaaaaacgg gttgaatttg aagtggctcc tttcggcatt atcgggctgg
     1981 agacctccct cgctttgacc attacccacc ttgtaaatcc cggccatgtg agtcttgaaa
     2041 aggcggtagc gcttctgagt cccggaggcg cccgcgtctg caatctgaat gcgggtacat
     2101 taaccccggg cgtgtcggcg gatattgcca tttttgatcc gcaggaaaag tggactgtcc
     2161 tcagtgaaga atttgcttct aaaagctgca attcgccttt tgtcggagca acgctgcagg
     2221 gtcgtgtgcg gtatacgcta tgcaaaggaa ctgtggtata cagcgcatcc tgagttcaga
     2281 tgtttcaagg aatgacactc ttgcctgaat accagggctt ccgtttttac aaggatgaaa
     2341 gcaccatgga acgtgcattg ctggaacagg gcgtcattct tgccgccgga tggaaacggc
     2401 gggctgagta tttaagcatc catccccggg atctcacggt tttggacaag cctcagggga
     2461 tcacgctttc tgattcagaa ttcctcgatt tacagagtgc gcgatctgaa gaagcctgcc
     2521 agtggtggga aaagcaggtg gacgaatatc ttcagtactg gacagcccgg cttaaagtat
     2581 ttgaggaaaa gcagagcagg ctcctgaatg ctgttgaaaa aggtcgcctc accccagcca
     2641 gagcaaacca gcgcgctcaa aagtttagcc gacagcatga acacgcccgc atgcagatac
     2701 agctttgtaa agaccttgca gcctgccttc agggcgatca ttcgctgccc cttcctgttc
     2761 tgccgttgtg tcagtactca gtatccaacc acacgcattc ccgtaattgg atgtccgcat
     2821 catctgcatt gaaaaaatca caatcttcca tcctctcccg gataacaggg cgctttgttg
     2881 cgggatctct ttcttttcac tctttgagca tcacagatcg cacagcactt gttttgtctc
     2941 ttttgttatc cgctttgatt gctgccggtg gttgcatgta tgcctttaga ggagaaaaga
     3001 tcgccattac cgtcaaggcg atagaccggg gcgaatatga ggtatccttt tccaacactt
     3061 ctccaaaaac actggcactt atccttcctt ataatggcgg agggcttgag aaaagtattc
     3121 cacctccgtt gggtgtcact gtggaactgt tggacaaaaa gaaaaacctg ccccttaacc
     3181 aatccattga gagcctctgg tattatcgtg acaaatcggc ttatctcaaa ggtcctgtac
     3241 tcctgagtcc cttttctcag gaagtacttt ctttgagact tgatcgggac gaaattgaaa
     3301 agaccagcgc ttccgaacaa ctacaaatcc gactgacgat ttttcgggca cccaatcgca
     3361 aaagcaagtc gttcactttc ccgctctcca gcgatgcagg ccatccaaaa gaatgactcc
     3421 gctcttggtt tcgatgtgta aagtgcggtt ccccccaaaa ttaggaccac gggctaaggt
     3481 ggaatcttta tcgctataat acccaaaaga aagggtagcg atgagcagga agcgcagtta
     3541 atagtacgtg tgctatacta tagtgaagtg tagaaattag cactattagc aaatagtgag
     3601 tcattctgca agaaaatgct tgcgacattc agggtaagga gcactaccat gaaaaatact
     3661 cattgttatg cgagatcact tattctattt ctggcgctca gcataagtat ttatgctctg
     3721 gctcagggac ctgtcgtatc caaagtgcag gttcagccgc ggacggatgg ctcgggtaat
     3781 gtggatatct attacgacct ccaccatccc aaagggttgg caagcacggc gagtctgcgc
     3841 ctctccaaag acggcggtgc cagtgacccc ttcaccctcg tccataccag tggccacaca
     3901 ggcaccaatg tcactccagg ttccaacggg catagcctct gggaggtggg caccgagttt
     3961 cccggtgaag agatcagccg ggctgtcatt gaagtagccg ctacgggtca taatgaggaa
     4021 gaaggggaaa tcattccagg tgaaatgatc accatccctg ccggatcttt taaaatggga
     4081 cgtccctata ccgatatagg atctccagac gaattgcctg tgcataccgt ctatctggat
     4141 cgttatcaga tcggaaagta tcaggtgacc aatgaggaat tcgccgctgt cctgaactgg
     4201 gcctatgctc gcaatctact caaaaactct acgggagaag cttatacaga tggcgctatt
     4261 tatgccaatg gccaacaact tgctgaaaca gagagttctt ccgacttctc tcagatcagc
     4321 tttgtcaata atcgattcag tgttcgaagc aggaagggcc tcgacgaccg gctctgtttc
     4381 atggctaatc atcctgttgt catggtgtcc tggtatggtg ctgtgtgcta ctgcaactgg
     4441 ctgagtgaaa aggaaggtct tgaatcctgt taccatacga cctcctggaa acgccatgag
     4501 cctgtgcgca atggctaccg tctgcccaca gaagcggaat gggaacgggc ggctgcctgg
     4561 gatcgtacaa aacactggcg ctatggcatg accagtgaca gcatccacgt taccaaagcc
     4621 aattacttcc ccaagaggtt tgccaatcct ctgggcttga agactgatcc ctatacctca
     4681 cctgtgggct ggtacaacgg tgtt
//
LOCUS       DAOPWB010000197         4554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_19092_length_4554_cov_4.414759, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000197 DAOPWB010000000
VERSION     DAOPWB010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19092_length_4554_cov_4.414759"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..1607)
                     /locus_tag="PKN92_12595"
     CDS             complement(342..1607)
                     /locus_tag="PKN92_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HNZ49674.1"
                     /translation="MKHMSWLLVLAMLAASPAFSGWTPVPEMELCPATVDDEDNPVNW
                     PKVNAMSGMPKIHNDEVTKNPWVAKPATFPDVAKEPYDFCGIFDVIYCSLGPAGSLSE
                     DILNFANLVQCMFADINGPVDLDADIPVTPNGVLDSYELAIIAAILNDVNHPLNADVT
                     ASFQGYFYEIKNLVMEALFVANVKGDKDLRGTLFGLAPYLGGSLVSVLAGFAVMGDPD
                     TDRALDALLGLLAEIGLEPPENGIASLGTPVPALGPLGDADGDGFTNKQEYNYFVNIL
                     EYSPEEFAAAALDPNAVPDTPPVPEVTIGGPTGRIAVGDTVTLSANVSVGSVTDYAWK
                     KNGVILDDEVGAALVLANVQLDDSGVYTAAVTVEYEDGNKALVVDTFEGSFTVTVLEY
                     TVPVGSALGLSIFAGACALAGAMGIRRRK"
     gene            complement(1986..2762)
                     /locus_tag="PKN92_12600"
     CDS             complement(1986..2762)
                     /locus_tag="PKN92_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNZ49675.1"
                     /translation="MQAEIISIGTELLLGQIVDTNAAHIAAALAEHGIPLYWKTTVGD
                     NAGRIEEALRRGLERSEILLCTGGLGPTVDDITRDCVARVLEQPLEFHPELYRHIEER
                     FRKFKRGLVPENNRRQACIPANALPLHNPCGTAPGLIAEAKNRCIICMPGVPAEMKTM
                     LSESVLPYLKERFNIQQVILHRTLLVRTLGESRVDALITDLFENSENPTIGLLATPGC
                     VQIRMTACAHDRNAALDLIAPLEQKIRERIAPYENGLEIK"
     gene            complement(2780..4210)
                     /locus_tag="PKN92_12605"
     CDS             complement(2780..4210)
                     /locus_tag="PKN92_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49676.1"
                     /translation="MQNSISDIARLCESWWSCPRIDSPVEQRSHVLQFLALLDWDLPI
                     PFSPGPAAEKAAALPYLLCADADTTIAAYFLRPGLLEQPIRLHEEKRDYSLLTRSLIA
                     ESDSPNIHYLFISDIGRSFLYHMPDGELLATADSPENFNRDLRRILRRGGVLQGALDG
                     LPRPSRCARAKRLDQWREYWKRELLRGFPASDEAVDLLFDRLTLLRYIFPRDVFRRTR
                     RRLQQRFYGLCRIDAPGDTRPAGDWGDQLRRLFHDMWLDWRIHLFAPHKELDALLSTA
                     PAMPGLLQASLLLADILFTDDVILESFNNGDAREKMRVRMVPDYNEERELLFARQSVE
                     TLDRLQVEIQIRDEGYRAIPFWIDRIIQSCEEIDARYSDACQHNADREEDDLFAWSDH
                     NSGRPDACTDPLGFICAQGVRVICTSPAQHRLALLLLTMRLVDLCRDKFPAREWLPDT
                     APVFTKTEKPLRDVPRIMLPPVTDLN"
BASE COUNT         1046 a         1108 c         1385 g         1015 t
ORIGIN      
        1 ggtcgacggg gggagaggca gggtcgacgg ggggaggagc aggaaggacg aggggtggcg
       61 agaaaaagca tatcggaaga aggaggctgg gacgacgggg agaggcagga atgacggggg
      121 gaggagcagg aaggacgagg ggcggcgaga aaaagcatat cggaagaagg aggcagggac
      181 gacggggaga cgggtggcgg agattggcgt tcccaggggg atccgcttgg gtgccaatag
      241 gtcgttagcg gggcgagaag gcatgtgatt tgtgaaagaa aaaagcgcag acatctgaat
      301 cagacgtctg cgcagaaaag gtcacagaaa ggattgtcta cttatttgcg gcgacggatg
      361 cccattgcac cagccagagc acaggcaccg gcgaagatgc tcagaccaag agcgctgcct
      421 acgggcacgg tgtactcaag cactgtaacg gtgaagctgc cttcgaaagt gtcaacgacg
      481 agggctttgt tgccgtcttc atactcaact gtgacagctg cggtgtagac accggagtca
      541 tcaagctgaa cgtttgcaag caccagcgca gcgcccactt cgtcgtccag aatcacgccg
      601 tttttcttcc aggcatagtc agtgacagag ccgaccgaca catttgcaga cagggtgaca
      661 gtgtcgccaa cagcgatgcg tcctgtcggg ccgccaatgg taacttccgg caccggagga
      721 gtatcaggca ctgcattggg atccagagca gcagcggcga attcttcggg actgtactcg
      781 aggatattta cgaagtaatt gtactcctgc ttgttggtaa atccatcacc gtcggcgtcg
      841 ccaagggggc cgagggcagg aacgggcgtt ccaagagaag caataccgtt ttcggggggc
      901 tcaagaccga tctccgccag cagaccgaga agtgcatcca gagctctgtc cgtatcggga
      961 tcgcccatca cagcaaagcc ggccagaacg gacacgaggg aaccgcccag atagggcgcc
     1021 agaccgaaga gcgtaccgcg caggtctttg tcgcccttca cattggcaac gaaaagagct
     1081 tccataacca gatttttaat ttcataaaaa tagccctgga aagaagcggt aacatcagca
     1141 ttgagcggat gattcacatc attcagaata gcggcaatga tggcaagttc gtaggagtcg
     1201 agcacgccat tgggggtaac gggaatgtcg gcatcaagat caacgggacc gttgatgtcg
     1261 gcaaacatgc actgcaccag attggcgaaa ttgaggatgt cttcggacag agagcccgcc
     1321 ggtccgagag agcagtaaat gacgtcgaaa attccgcaga aatcataggg ctcttttgcg
     1381 acatcgggaa aagtcgccgg ctttgccacc cagggattct tcgtgacctc atcgttgtga
     1441 attttgggca ttccggacat agcattcact ttaggccagt tcacgggatt gtcttcatca
     1501 tccaccgtcg ccgggcagag ctccatttcc ggtacgggcg tccagccgga aaaggcgggg
     1561 cttgcagcga gcatcgccag tacgagtaac caactcatgt gtttcatctt tcgtcctcct
     1621 gtttgaatac ctttgtaata aggttagacc aagaacatca acgcgttatt tatagcagtt
     1681 ctttttcggc aggcacaccc catgtgtgcc tcgtataaag aacatataac cagaagattc
     1741 aacttagcac ttggcgttct gattacataa tacaccaaaa acaatccgtt gtcaagcttg
     1801 ttgtgcattt ttatcggaga agtaaaaact tcatgattgc ggcctttgtc tgtgcggggc
     1861 acagggcccg tattctacag tcacctcagg cgtttttggt gcttttcggg cacccgggat
     1921 tgtacgccct attttccggg tttgcgcctt tgtattctgt cggtcttgcg gtctttttcc
     1981 ggtttttatt taatttcgag tccgttttca tacggtgcga tgcgttctct gattttctgt
     2041 tccaagggtg cgatgaggtc gagggcggcg tttctgtcgt gcgcgcatgc ggtcatccgg
     2101 atctgtacgc atccgggtgt ggcgaggagc ccgatggtcg ggttttccga attctcgaag
     2161 aggtcggtga tgagtgcatc gactctggat tcgcccagtg tacgcacgag cagggttcta
     2221 tgcagaataa cctgctgaat attgaagcgc tctttgagat agggcaaaac gctttcgctg
     2281 agcatggttt tcatttctgc cggtacgccg ggcatacaga taatacagcg gttctttgct
     2341 tcagcgatca atccgggagc ggtcccgcat ggattgtgca ggggtaatgc atttgcgggg
     2401 atacaggcct ggcgtctgtt gttttcgggg accagccctc ttttgaattt tcggaatcgt
     2461 tcttcgatgt gtctgtaaag ttcggggtga aattcgagtg gctgctcaag cacccgggcg
     2521 acacagtctc tggtgatgtc gtccactgta ggaccgagtc ctccggtaca gagcaggatt
     2581 tcggatcttt caagcccgcg gcgcagcgct tcttcaatac gtcctgcgtt atcgccgact
     2641 gttgttttcc agtaaagcgg gatgccatgt tctgcgaggg ctgcggcgat gtgtgcggcg
     2701 ttggtgtcga cgatctgtcc gagaagcagt tctgtgccga tggatataat ttctgcctgc
     2761 atgggagggc gcgactttct taattaaggt ctgttacggg cgggagcata atgcggggca
     2821 catcgcgaag cggcttttcc gtttttgtga aaacaggtgc tgtatcggga agccattccc
     2881 gtgccggaaa tttatcacgg cagaggtcaa cgagccgcat ggtgagcagg agcagtgcga
     2941 gtctgtgttg tgcggggctg gtgcatatga cccggacacc ctgagcgcag atgaagccca
     3001 ggggatccgt gcaggcatcg ggtctgccgc tgttgtgatc ggaccaggcg aagagatcat
     3061 cctcttccct atcggcgttg tgttggcatg catcgctgta tcgggcatca atttcctcac
     3121 agctttgaat gattcggtca atccagaaag gaatagcccg gtagccctcg tcccggatct
     3181 gaatttcaac ctgcagccgg tcgagtgttt cgacggactg gcgggcgaag aggagctcgc
     3241 gctcttcgtt gtagtcgggc accatgcgta ctctcatttt ttcccgggca tcaccgttgt
     3301 tgaaactttc gagaatcaca tcatcggtga aaaggatatc tgcaagcaga agagaagcct
     3361 gcagcagtcc gggcatggcg ggggcggtgc tgaggagtgc atcgagttct ttatggggtg
     3421 cgaaaaggtg tatgcgccag tcgagccaca tatcgtggaa aaggcgtctg agctgatcgc
     3481 cccagtcgcc ggcaggtctg gtgtccccgg gtgcatcaat ccggcagaga ccgtaaaagc
     3541 gctgctgcag gcggcggcgt gtccggcgga aaacatcgcg ggggaaaatg tagcggagca
     3601 gtgtaagcct gtcaaagagc agatcgacag cttcgtcgga agcgggaaag cctcggagca
     3661 actcgcgctt ccagtattcg cgccactggt cgaggcgctt cgcacgggca cagcgggacg
     3721 gacgtggcaa accgtcgagc gcaccctgca gtacgccgcc gcgccggaga atccgcctca
     3781 gatcccggtt gaagttttct gggctgtcgg cggtggcgag aagttcaccg tccggcatat
     3841 ggtagagaaa actgcggccg atgtcgctga tgaagagata atgaatattg ggtgaatccg
     3901 attccgcaat gagcgaccgg gtaagaagac tgtaatcgcg cttttcctcg tgcagccgga
     3961 tgggctgttc gagcagcccg ggacgaagga aataagcggc aatggttgtg tcggcatcgg
     4021 cacataagag atacgggagg gctgccgctt tttcggcggc aggtcccgga gaaaagggaa
     4081 tgggcagatc ccagtccagc aatgcgagaa actgcaggac atgactgcgc tgttccaccg
     4141 gtgaatcaat acggggacaa ctccaccagg actcgcagag tctggcgatg tcggatatgc
     4201 tgttttgcat gtaaagccct tggatcctcc tgggtctgca ttaaagcaga ctaccatata
     4261 ttgcaccgtg ctgactatta tcatatacaa attgtgtctg aaggtcaaga ggaaactgag
     4321 aaagggcggc tgtgaagaag acaagcattt ggatctgtat cggtattatg tcccgggatg
     4381 tatctccata aacatggatc acacacgccc atgccaatgc ggagattggc acgcaagcga
     4441 gtcatgaaaa agacaggcgc ctggatctga gtcggagtaa tgtcccggga catacctcct
     4501 ccatcagtcc tacacacgac gctgtcattt ctccttatcg ccgttacgtc cacc
//
LOCUS       DAOPWB010000198         4553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_19097_length_4553_cov_4.939084, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000198 DAOPWB010000000
VERSION     DAOPWB010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19097_length_4553_cov_4.939084"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PKN92_12610"
     CDS             complement(<1..1056)
                     /locus_tag="PKN92_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49677.1"
                     /translation="MRRLSFVGLAGRNLRYHWRIHSGVFAGILLTTAVITGALILGDS
                     TRFTLRNIAVARLAGAHYACQLPNRYFEASLAHRMAEEGQGVPAALLRLQGMALQSGA
                     PDPLYRVNRVQVLGVDDAFWQLSGTDAPQWNAQEVIVNEHLARALHAGRGDRFALRVV
                     KPGLMAFDAPLSPGGDAAAIRVQVKVGDIVSDNGAGRFNLNNEQRTPYNVFVPLRWLQ
                     ELVDLEGKANLLLAGETWSEGQLQAELKKVMHLRDAGFQIRALSDQSYLLESERIFLD
                     RTIADAALTLPQAEGSLAYLVNSISGERHSTPYSFAVADASLAQLNDEEAIINRWTAE
                     KLDVSMGDTITVRWFVVT"
     gene            complement(1053..1745)
                     /locus_tag="PKN92_12615"
     CDS             complement(1053..1745)
                     /locus_tag="PKN92_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNZ49678.1"
                     /translation="MTQVLVPALMELNAVTYAYAGLSGSIDVLKGVTLRIKSGECIAV
                     VGPSGSGKSTLLNCMGGLDKPGSGSILFDGTHLSEYSPEELARFRNREIGFVLQQHHL
                     LPQCSVLENVLIPTLVAPHARESGERALMLLHQVGLAHRLHHRPQELSGGECQRVAVV
                     RALINNPRLLLADEPTGSLNEESAVALVDLLMTLNREQGMAVVMVTHSPDLAERLGRT
                     YRLHDGKLEMLP"
     gene            complement(1742..3145)
                     /locus_tag="PKN92_12620"
     CDS             complement(1742..3145)
                     /locus_tag="PKN92_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ49679.1"
                     /translation="MKKVISSFSFVLFLISLTLLFLLIKKKEMETVPLSSDPVTPIEA
                     MLAVPEKEEPIGSGEKSPDLPDEDQTNTAVLSVSMAQTHAASPTGAAESGTENLPWHS
                     YHGSSTLTGSSPVSLPGSLVPLWQTDAEGAVLGTPVYSGSAVFFATVKGSVFAVDPEG
                     EFLWSAKIITGEKADGTPRFASFEVPLLYLEGTVYACDADGTVIALDAAAGNELWRSF
                     LDITVLGAPNYKLVSRNGMAPEISIYIIDQTAGALYCLDGATGEKKWISETGVGRCEA
                     SPGVYDEYVLFGSCASALHVFSSSTGKHLYDLPLGNDAQVASGVAISDGKGLSGSRNG
                     NLVLIDLKEEKTIWDTRLSRHEIFSTPAVSNQWVVVGDENGSLYGVSFATGEKRWETS
                     LGGSVLSPVIVGDHVAVSADGSLYLLTLASGERSWSYALSDEITSPCVAGNMLVVGSS
                     DATVTAFAGNRKGEEGI"
     gene            complement(3193..4527)
                     /locus_tag="PKN92_12625"
     CDS             complement(3193..4527)
                     /locus_tag="PKN92_12625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNZ49680.1"
                     /translation="MKIVHIVPGSGGTFYCQNCLRDTALVRALRAQRHDVMLTPLYLP
                     LFADDAALAEEVPVFFGGINVYLQQRFPFFRKTPAWLDRLFDASWLLRLAARQESSTS
                     ARDLGAMTLSMLQGKTGNQAKEVARLVDWLKDQEKPDVVHLSNALLLGLAPQLREELD
                     CPVLCTLQDEDTWMEAMRPDEAEACWNAMREAGESVDLFIAVSQWYADKIQKAMRIPA
                     ARLKVVHLGVENEIFQPDALPFDPPVIGYLSRLAPGQGLDRLIDAFIALKKQSEFHRL
                     RLRATGGITVAHQPFIQAMRQKLEAAGCLDDVEFLEDFGKAEREAFLRSISLLSVPAP
                     GGEAFGAFILEAHANGVPVVQPAEGAFPEVVALTGGGILYDPAQPGAYEEALAELLSR
                     PDYAASLGETGRRAVEKRFNIRVMADQLAAHYQECTEKTGTEPAVISTVCGK"
BASE COUNT         1087 a         1343 c         1148 g          975 t
ORIGIN      
        1 cgtcacgaca aaccaacgga ctgtaatcgt gtcgcccatg cttacatcca gtttttcagc
       61 agtccaccgg ttgatgattg cttcttcgtc attcagctga gcaagcgaag cgtctgccac
      121 tgcgaatgaa taaggagtgg aatgcctttc gccggaaatg gaattcacca gatacgccag
      181 acttccctct gcctgcggca gggtcagggc ggcgtctgca atcgtcctgt ccagaaagat
      241 tctctcagat tccagaagat aagactgatc ggacagcgcg cggatttgaa atcctgcatc
      301 ccgcagatgc atcacctttt tcagttctgc ctgcagctgc ccttcgctcc aggtctctcc
      361 tgcaagaaga aggttggcct tcccctccag atccaccagt tcctgaagcc agcgcaaagg
      421 aacaaagacg ttatagggtg tcctctgttc attatttaaa ttaaaacgcc cggcgccgtt
      481 gtctgacaca atatctccga ctttcacctg tacacgtatc gccgcagcat cgccgcccgg
      541 cgacagcgga gcatcgaaag ccatcaggcc gggtttcacc acacgaagcg caaaacggtc
      601 tcccctgcct gcatgaagcg cccgggcaag atgctcattc acgatcactt cctgtgcatt
      661 ccattgtggg gcgtcagtgc ccgataactg ccagaatgca tcgtcgaccc ccagcacctg
      721 cacccgattc acccgataca gcgggtcggg cgcgccggat tgaagcgcca tgccctgaag
      781 ccggaggagg gcggcaggca ctccctgccc ttcttctgcc atcctgtgtg ccagtgaggc
      841 ctcgaaatac cgattaggca gctggcaggc ataatgggcc ccggcgaggc gggcgacagc
      901 aatattccgc aaggtaaacc gcgtggaatc tccaagaatc agggcgccag tgatgactgc
      961 tgttgtaagc aggataccgg caaatacacc gctgtggatg cgccagtgat agcgcagatt
     1021 ccgacccgcc aacccgacaa aagacaggcg cctcatggca gcatttccaa tttcccgtca
     1081 tgaaggcggt aagtacgtcc cagacgctca gccagatcag gtgaatgggt caccatcacc
     1141 accgccatgc cctgttcgcg attcagcgtc atcagaagat cgaccagcgc gactgccgac
     1201 tcttcattca gtgacccggt aggctcgtcc gcaagcagaa gacgggggtt attgatgaga
     1261 gcacgcacaa cggcaacacg ctggcattct ccgccggaga gttcctgagg cctgtggtgg
     1321 aggcggtgcg ccagtcccac ctgatgaagc aacataagag cccgttctcc agattcgcgg
     1381 gcatggggag ccacgagcgt agggatcagc acattttcca gcacggaaca ctgcggcagg
     1441 aggtgatgct gctgcagcac gaacccgatc tctctgtttc tgaaccgtgc aagttcttcc
     1501 ggcgaatatt cggacaggtg cgttccatca aacaaaatgg atccggagcc gggcttatcg
     1561 agcccgccca tgcagttcag aagcgtgctt ttcccgctcc cggaagggcc gaccacagct
     1621 atacattccc cgctttttat gcgcagggtc acgcctttaa gcacatcgat ggatccggac
     1681 agccctgcat aggcatatgt cacggcattc aactccatta gagccggtac cagtacctgt
     1741 gtcatatgcc ctcttctcct ttcctgttcc ctgcaaaagc tgtgacggtt gcatcgctac
     1801 ttccgacaac aagcatattt cctgccacgc acggcgaagt gatttcatca ctgagtgcat
     1861 aagaccacga ccgctctccc gatgccagtg ttaaaagata gagactgcca tcagcggata
     1921 cagcgacatg gtcaccgaca atcacaggag acaaaacaga gccgccgaga gaggtttccc
     1981 atcttttttc accggttgca aaagacactc cgtaaagact tccgttttca tcgccaacaa
     2041 cgacccattg gtttgaaaca gcaggtgtgg aaaaaatctc atgcctggac agccgtgtat
     2101 cccatatcgt tttttcttct tttaagtcga tgagtacgag gtttccattt ctgcttccgg
     2161 acagtccttt tccgtcactt atggcaaccc cggaagccac ctgcgcatca ttgcccaacg
     2221 gtagatcgta gagatgcttc cctgtgctgc tggaaaaaac atggagcgct gaggcacagc
     2281 ttccgaaaag cacatactca tcatagacgc ccggcgaggc ttcgcagcgt cccacacccg
     2341 tttcggagat ccattttttt tctcctgtgg caccatccag gcagtacagc gcacccgcgg
     2401 tctgatcgat gatatagata gaaatctcgg gggccatacc gttccgggaa accagtttat
     2461 agttgggcgc acccagaaca gtgatatcca gaaaggagcg ccacaattca ttgcctgcag
     2521 cagcatcgag ggcaataacc gttccgtccg catcgcaggc gtaaacggtg ccttccagat
     2581 aaagaagggg cacttcaaaa gaagcaaacc ggggcgtgcc atccgctttt tcgccggtaa
     2641 taatctttgc cgaccagaga aattctcctt ccggatccac agcgaaaaca gagcctttca
     2701 ccgtggcaaa gaaaacagca cttccggaat aaacaggtgt cccaagaact gccccttccg
     2761 catctgtctg ccacagcggc acaagacttc cgggcaggga aaccggggaa gagccggtca
     2821 gcgtggatga gccgtggtaa ctgtgccagg gcagattttc cgtaccggac tccgctgcgc
     2881 cggttggaga tgctgcatgt gtctgtgcca tggatacgga taataccgct gtattcgtct
     2941 ggtcttcatc cggcagatcc gggcttttct ctccggaacc tatcggttct tctttttccg
     3001 gcacagcgag cattgcttca atgggcgtta ccggatcaga ggaaagcgga acggtttcca
     3061 tttctttctt tttaataagc agaaagagga gtgtaaggga tatcagaaaa aggacgaaag
     3121 agaaggatga aattactttt ttcatagact gctccagaat ttcaggcttg ccttttcccg
     3181 atagccttct gttcatttgc cacagacagt tgagataaca gcgggttcag tccctgtttt
     3241 ttctgtacat tcctgataat gggcagcaag ctgatctgcc atcacgcgga tattaaaacg
     3301 cttttccaca gcccggcgcc ctgtctctcc cagcgatgcg gcataatccg gacgggaaag
     3361 caattcagca agtgcttctt cataggcgcc cggctgcgcc ggatcgtaaa gaatcccgcc
     3421 gcctgtaagc gccaccactt caggaaaggc gccttccgcc ggctgcacca caggcacgcc
     3481 atttgcatgt gcttccagaa taaaggcgcc gaaggcttca ccgcccggtg cgggcacgga
     3541 aagcagcgaa atggagcgaa gaaacgcttc tctttccgct ttcccgaaat cctccaaaaa
     3601 ctccacatca tccagacaac ctgcagcctc cagcttctgc cgcatcgcct gaataaaagg
     3661 ctgatgggcg actgtaatac cgccggtggc acgcagccgc agccgatgga attcgctctg
     3721 ttttttcaat gctataaacg catcgatcag gcgatccaat ccctggccgg gcgccagccg
     3781 tgacagatat ccgataactg gcggatcaaa aggaagggca tccggctgaa aaatctcgtt
     3841 ttccacgcca agatgcacaa ctttaagcct tgctgcagga atccgcatag ctttctgtat
     3901 tttatccgcg taccactggc tcacggcgat aaaaagatcc accgattccc ctgcttccct
     3961 catggcgttc cagcaggctt cggcctcatc agggcgcatg gcttccatcc aggtatcttc
     4021 atcctgcaag gtgcagagca cagggcaatc cagttcctct ctcaactgag gagccagtcc
     4081 gagaagaagc gcattggaca ggtgaaccac atccggcttt tcctgatctt taagccagtc
     4141 aaccagacgc gccacctctt ttgcctgatt gcctgttttg ccctgaagca tggacagcgt
     4201 catcgctccc aggtcacggg cgctggttga actctcctgc cgtgccgcaa gccgcagcag
     4261 ccacgaggca tcaaagaggc gatccagcca ggcaggcgtt tttcggaaaa agggaaagcg
     4321 ctgctgcagg taaacattga ttccgccgaa gaaaacaggc acctcttccg ccagcgcggc
     4381 atcgtccgca aaaagcggaa gatagagcgg ggtcagcatc acgtcgtgcc tctgcgcccg
     4441 cagagcacgc accagcgccg tgtcacgcaa acaattctgg cagtaaaaag taccgccgct
     4501 tcccggtacg atatggacta ttttcacgga gaacattcct cctgatcatc aaa
//
LOCUS       DAOPWB010000199         4527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_19235_length_4527_cov_5.077147, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000199 DAOPWB010000000
VERSION     DAOPWB010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19235_length_4527_cov_5.077147"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..762)
                     /gene="recO"
                     /locus_tag="PKN92_12630"
     CDS             complement(100..762)
                     /gene="recO"
                     /locus_tag="PKN92_12630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNZ49681.1"
                     /translation="MTYEHTQALVARKVDFGESSRIVTLITPERGITPGMAKGSRRKG
                     SPLTALFDTYNLLDITLIWKDSRNVQTITDAALLKSYGVVKRDIIRSAAAAVLLETAL
                     CASVEGCPNSELFTQSKAGLEQLDSPDAEPLPSLCLALFQVLETSGVSASSGTLAELP
                     ITKNYSVRQRALIEEGLRCLEDQQKPQEKEIVYLLHYLHDFTQHHFEKKLRSVVFLNS
                     LL"
     gene            complement(781..2061)
                     /locus_tag="PKN92_12635"
     CDS             complement(781..2061)
                     /locus_tag="PKN92_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49682.1"
                     /translation="MKHNTRWLFLLLLVCVIPGVHAQSLKAGAAMRIITPDPLLPASG
                     GIGTPRPTTDKRGELSVRALVFDSGDTAVALVAVDNLGWPAYWGDKTRKRVSAIPREN
                     IIIAATHTHSAPDAYCFPDLEGKHHADLNYLEWVCVQAADAINEAWEKRQPARLKIAV
                     GKAEGKIAYNYYAPQLYDPRCGVIQAVASASDGAEKVIATFVNYAIHPEVLGSDKGII
                     SPDLCGPFYDRIESKAGGIALFMNSAQGGMVTADNRSEQGDMEIWEECVRIGELLADE
                     ALRIVADAEEQEQPVLHCSARIISLPVENILMQLLMQSVPMADPLGEYDKVKTQVNLL
                     SVGTAQLLTIPGEALPNIGFYLKRKMPARHPFLLGLANDAFGYMLTKYDFNSFERYDY
                     ISETSLGEMAAEKYVEEALDLIKSSPPPDRPLAK"
     gene            complement(2221..2778)
                     /locus_tag="PKN92_12640"
     CDS             complement(2221..2778)
                     /locus_tag="PKN92_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNZ49683.1"
                     /translation="MSKKQLVLLLLLMICLVLPGCPPDVPPIVVPPEEDECIEPTDPP
                     DDGQVVIREEVEAAIFEYVNAVRVEEGLTPLIYDADVSSVALAHSKDMFDRSYLDHKN
                     PDGLRPCDRLTQAGITWSHVGETIFYTEGYRPEKIAAMTIDAWLESPSHRKVLLNPVY
                     THTGLGVFGTPCGRFYVTEMYLKKK"
     gene            complement(2889..4034)
                     /gene="pelA"
                     /locus_tag="PKN92_12645"
     CDS             complement(2889..4034)
                     /gene="pelA"
                     /locus_tag="PKN92_12645"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HNZ49684.1"
                     /translation="MPHLLLLFILSASAVCAEKAAPVSVSMFFDNIHHYQALADSKTY
                     PRFKETQWADIADNILLMQRDNGGWASNWDPLRVIPPEEISVHQDRKAAKDTTFDNRS
                     TYTHMLYLSEVYKRTGEERFRAGAEKALQFILDAQYENGGWPHSYPQKEGYLSRITIM
                     DDVMVGVLSALRKVCSEESSVSFLDDALRLRAEKAWQCGHRCLLDLQVRVDGMPTVWA
                     GQYDERTLEPAAGRSFELPGLVSLESATVVHYLMKEAPQTEETVYAVECAIDWFKKSQ
                     LNGLRIDRIDCEPVQYKYRVSRKDVVAVPDPEAPPLWARFYEIGTNRPFMANRDGTKV
                     YSIEELEHERRSGYAWYGTGPALLLEEYASWKKPQTICSEARECVEK"
BASE COUNT         1147 a         1153 c         1027 g         1200 t
ORIGIN      
        1 catagtatac acaaggcaaa gagggaatgc aaacttttat tcctcttttt ttgtcctcac
       61 catcagagcc gctttaaagc ctttatttac agggtgaatc tatagaaggg aatttaaaaa
      121 aacaacgctt ctcagttttt tttcaaaatg atgttgagta aagtcgtgca ggtaatgcag
      181 taaataaacg atttcttttt cctgtggctt ttgctgatct tccaggcaac gcagtccctc
      241 ttcaatcagt gcccgctgtc ggacagagta attttttgtg atcggaagtt cagccagcgt
      301 gccggaactt gcggagactc ccgaagtctc caacacctga aaaagcgcaa ggcacaagga
      361 gggcagaggt tctgcgtcgg gcgagtcaag ttgttcaagt cctgctttgc tttgtgtaaa
      421 caattcagag ttcgggcacc cttcaacgga tgcacacaat gctgtttcca gcagaaccgc
      481 tgcagcagcg gaacgtatta tgtcgcgttt aacgacacca taacttttca acaacgcagc
      541 atccgttatg gtctggacat ttctgctgtc tttccaaatg agggtaatat caagaagatt
      601 gtatgtatca aacagtgcag tcagaggact cccttttctt ctgctgccct ttgccatacc
      661 gggcgtaata ccgcgctcgg gcgtgatcag cgtaacaatc ctgctggatt caccgaagtc
      721 aactttgcgt gcaaccagtg cctgagtatg ttcgtatgtc aagtaacact ccccgccttt
      781 ttatttcgcc agaggcctgt ccggaggtgg tgaacttttt attaaatcca gtgcttcttc
      841 gacatatttt tccgctgcca tttcgcccag acttgtttcc gaaatatagt cgtatcgttc
      901 aaaactgttg aaatcatatt ttgtcagcat ataaccgaag gcatcattgg caagaccgag
      961 cagaaacggg tgccttgcag gcattttccg ttttaaataa aaaccgatat tcggaagtgc
     1021 ttcaccgggg atggtaagca actgagcggt gccgacagac aaaagattca cttgggtttt
     1081 caccttatcg tactcgccca gcggatctgc catgggaaca ctctgcatca gaagctgcat
     1141 caggatgttt tctaccggca gagaaatgat ccgggcactg caatgcagca caggctgttc
     1201 ctgttcttct gcatcggcaa caatgcgcag cgcttcatcg gcaagcaatt cgcctatacg
     1261 cacacactcc tcccaaatct ccatatcacc ctgctccgac cggttgtcgg ctgtgaccat
     1321 tcctccttgt gcactattca taaaaagagc gatgccgccc gctttgcttt cgatgcggtc
     1381 ataaaaaggc ccgcaaagat cagggctgat aatccctttg tcgctaccga gcacctccgg
     1441 atgaatagca taatttacaa aagttgcaat cactttttcc gctccatccg aagcagaagc
     1501 gaccgcctga atcaccccgc agcggggatc atacagttga ggcgcataat agttgtaggc
     1561 aatttttcct tccgctttac cgacagcaat tttcaatcgg gcaggctgtc ttttttccca
     1621 cgcctcatta atggcatctg cagcctgtac acaaacccat tcaagataat tcaaatctgc
     1681 atgatgtttt ccttcaaggt caggaaagca ataagcatca ggggcgctgt gcgtatgtgt
     1741 tgcggcaatg ataatatttt cccgcggaat cgctgaaacc ctctttctgg ttttatctcc
     1801 ccagtaagcc ggccagccga gattatccac tgctaccagc gcaacagcag tatcgccact
     1861 gtcaaaaacc agggcgcgca ccgacagttc tcccctttta tccgtcgtcg gtcggggcgt
     1921 tcctatgccg cccgaagcgg gaagaagcgg atcgggcgta ataatgcgca tggcggcacc
     1981 tgctttcagt gactgggcat gcaccccggg aatcacgcaa acaagcaaca gaagaaataa
     2041 ccagcgggta ttgtgcttca taaaacagcg aatgctcctt aaaaaagaat gcaggtgcaa
     2101 aagaaaaaga tacacctgaa gtaaaataga ggcttctttt gtggcacatt tttttaagat
     2161 atcagcactg cctcagaaac cttttggatc tttctgtgtt taatttgact cttctctttt
     2221 ctactttttt ttcagataca tttccgtcac ataaaagcga ccgcatgggg taccgaagac
     2281 acctaaacca gtgtgcgtat atacgggatt aagcaacact ttccggtggg acggactctc
     2341 cagccaggcg tcaattgtca tggcggcaat cttttccggg cgatagccct cagtataaaa
     2401 gattgtttct cctacatggc tccaggtaat cccggcttgc gtgaggcggt cgcagggacg
     2461 cagcccatca ggatttttat gatccaggta tgatcggtca aacatgtctt tagagtgcgc
     2521 caatgccaca gaggaaacgt ctgcatcata aatcaagggg gtgagcccct cttcgactcg
     2581 gactgcattg acatactcaa atattgccgc ttctacctcc tcccggataa caacctgccc
     2641 gtcatcaggc ggatctgtcg gctcgatgca ttcgtcttcc tcaggcggaa caactatggg
     2701 cggcacgtct ggcggacagc cgggcagcac caggcaaatc atcaaaagca ggagcagaac
     2761 aagttgtttt ttactcatgg cacacattct ccagcatccg gccaatcata accggcaaat
     2821 gtttcatcag atcaaaaagg gcctttgctg tctgttttta ttttaccatt tttcgaggca
     2881 ttcgtaaatt atttttcaac acattctcgt gcctctgaac aaatagtttg cggctttttc
     2941 caggaagcat attcttcaag cagcagggca ggtcctgttc cgtaccacgc atagccggaa
     3001 cgcctttcat gctccagctc ctcgatagaa tagacttttg tgccgtcccg atttgccata
     3061 aacggccggt ttgtgcctat ttcgtaaaaa cgcgcccaga ggggaggtgc ttccggatcc
     3121 ggcacagcaa cgacatcttt tctggacact ctgtatttgt attgaacggg ctcgcagtca
     3181 atacgatcaa ttcgcagtcc gtttagctgt gattttttaa accagtcaat agcgcattcc
     3241 accgcataaa ccgtttcctc tgtttgaggg gcttccttca tgagatagtg cacaacggtg
     3301 gcactttcaa ggcttacaag tccgggcagt tcaaaagagc gtccggcagc cggttccagg
     3361 gtacgctcat cgtattgccc cgcccacaca gtaggcatgc cgtccacgcg cacctgcaga
     3421 tcaagaaggc atctgtgccc gcattgccag gctttttcag cacggagccg caaagcatca
     3481 tccagaaaag aaacgctgct ttcttccgaa cacactttcc gaagagcgga aagcacccca
     3541 accatcacat catccatgat ggttatgcgg gacagataac cctctttctg aggataggaa
     3601 tgaggccagc cgccgttctc gtactgggca tccaaaatga attgcagcgc tttttcggcg
     3661 cctgcccgaa atcgctcttc gcccgtacgt ttataaactt cgctcagata aagcatgtgc
     3721 gtgtaggtgc tgcggttgtc gaaagttgta tcttttgcag cctttcggtc ctgatgcacg
     3781 gagatttctt caggcgggat cacacgcagc gggtcccagt tggaagccca gcctccgttg
     3841 tcccgctgca tcagcaaaat attatccgca atatctgccc attgtgtttc tttaaaacgg
     3901 ggatacgttt tgctatctgc gagtgcctga taatgatgga tgttatcgaa aaacatgctg
     3961 acagataccg gcgctgcctt ttctgcacag acggcagaag cggacagaat aaaaagaagt
     4021 aagaggtgcg gcataggaaa aaagcctttc gtgtttccct atttgcagga cagcaacagt
     4081 tgaattatgc tttaatgcac tatagagaaa tcatccggat tatgcaatgt tttgaccgga
     4141 tgcatttttg tgcgataact gaaaactgct ccccctattt ttatttttta aaacatgccg
     4201 tttaacagcg tgcagcctgc tgaagcattt gactaaatag acaggcactt cgtattattg
     4261 ctctgtccgc cagatgcagt taacgaaccg gatgagaaag gatcttttag tggtgttgga
     4321 attcacaaca gtccacgttt tttctctctc gtaaaatcac atacagcaac ggaatgtttt
     4381 tccctgcttc ttctttgacc cggcttactg ccgctgacct gcccgatcaa acaatttgcg
     4441 atgcatagca taatcaggaa aagaaatgaa tgctaaaaaa gtggcagttg tattgcctgc
     4501 ctataattgc gccgctacgt tgggtgg
//
LOCUS       DAOPWB010000200         4490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_19445_length_4490_cov_5.000000, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000200 DAOPWB010000000
VERSION     DAOPWB010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19445_length_4490_cov_5.000000"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2180
                     /locus_tag="PKN92_12650"
     CDS             <1..2180
                     /locus_tag="PKN92_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNZ49685.1"
                     /translation="GADEMNLAIIHGLGRLYRATGEDRYLAMMRVVEEDWKETGDYFR
                     QGLAGVPFFRTPRPRWESLHDVQGLVELYRITGNEDYKTAFVQLWRSIAAYDRHNTGG
                     FTTDEAAIGNPYTPGAIETCCTTSWSALTLDMLLLTGESQAADELEWSLYNSILGSQH
                     PTGRWWTYNTPMDGKREASAHTIVFQSRAGTPELNCCSVNAPRGLGMLSEWALLSDAS
                     GALIVNYYGPMSAGVMLQDAGKVAVNVESAYPAEASATILLGLDAPASFPLRLRIPAW
                     SKHTTVTVAGDVLDGIGPGSYLTIERTWSDNDRIELAFDFSIRTWVGDLDQAECVSLF
                     SGPILLAFDQRDNSYDCDELAPLDFIHLSYTRIADNSRFSWMPLFEFKDVNGHAVRLR
                     DFASAGATGTEYRSWLPAANTPPPPFHLLEPAPQAAIPAGPFKFTWTGPRDGAAQTYT
                     VEIRDTNGALTWQSEPTRQPWCVVRAELTPGTPYAWRVLAQNTHGRIFNEEGPRRFVV
                     DPDMPNLFVDHPALLSFRKDGLAASGLLDGDGSPEYGYLDEACGITRGTDRNGKDGGA
                     VRFAGDGLLRYRMPYFPGEHYSFLAWVCPDILPETGLAQVFSAWSRSGDDPLRVVIEG
                     AQIYARIEGSGGANTKGQPLTAGEWVHIAAVKDGARLRLYCNGTLVDDTAAPAKLVTT
                     AEDFALGANPHFSGTEHFTGRIDDFAFYARALSEAEIHKMMKP"
     gene            complement(2292..2990)
                     /locus_tag="PKN92_12655"
     CDS             complement(2292..2990)
                     /locus_tag="PKN92_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HNZ49686.1"
                     /translation="METRKETVKNIYKALKRHFGLTHWWPGDTPFEIALGAILTQNTA
                     WANVEKALDNMKAKDWLSPEALTEAASEEVEEALRPSGYFRQKTERVRLFCSHLLTHY
                     QGSMKKLGERPLKELREELLSLKGIGPETADDILLYACHKKIFVVDAYTKRIFSRCGL
                     VSEKIAYHPLQALIEKNFAGTVKDYQEFHGLIVWTAKDFCRKTPACRGCPLALPEQRG
                     QAFPCHRSAIPWDG"
     gene            complement(2966..4039)
                     /locus_tag="PKN92_12660"
     CDS             complement(2966..4039)
                     /locus_tag="PKN92_12660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNZ49687.1"
                     /translation="MREQDLFYASVALVSFLAVFMLVPLCRKAALKLGALDQPDQERK
                     VHRRAVPYLGGLSFFLAWLLLLIVFAFAAPHRLLPEFYPLAAGGFLIVLLGVYDDICD
                     LTSKIKLPLELLLCASLYFWGFKSEVLSNPFGGTVYIGGFALVVTALWIAGIMNAVNF
                     ADGLDGLAAGLTCICSAVLFFVSFRSGSVFSCTIMAFLFGITLAFLCYNFHPASIFMG
                     DAGALFLGFLLGASTVIERQKGTAVIALSVPVMVMAVPLLDTVLSFMRRLRRARSGRF
                     FTPDREHLHHRLLALGLSQKQVVLSLYAVALGAGILAVLFDRVTSGYKMIVLAAVGAA
                     AFCAVALLRLFESRQQWKREKKQ"
     gene            complement(4036..>4490)
                     /locus_tag="PKN92_12665"
     CDS             complement(4036..>4490)
                     /locus_tag="PKN92_12665"
                     /inference="COORDINATES: protein motif:HMM:NF027047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="interleukin-like EMT inducer domain-containing
                     protein"
                     /protein_id="HNZ49688.1"
                     /translation="DSRRGVHLFAGEKDLGRRERGMHIALLDAGSGALLAAACFDVWE
                     RREEGQRLFSFLTEAPEGCIGLFAVYDDGAGVVTHDLEEAFLFFGLDRCLMDERKMRV
                     TAIRSSLAAIGVKGAVPGSALQVWSPQWYHGRRGHPVLCAAIPGGKAP"
BASE COUNT         1040 a         1371 c         1220 g          859 t
ORIGIN      
        1 ccggtgcgga tgaaatgaac ctcgccatca tccacgggct cggcaggctt taccgtgcta
       61 caggcgaaga ccggtatctc gcgatgatgc gtgtagtcga agaagactgg aaagaaacag
      121 gggattactt tcggcagggc cttgccgggg tgcctttctt ccggacgccg cgaccgcgct
      181 gggaaagcct ccatgatgtg caggggctgg tggaactgta ccggataacc ggcaacgaag
      241 actacaagac ggccttcgtt caattgtggc gcagcattgc cgcctatgat cggcacaaca
      301 ccggtggctt cacgacagat gaagccgcca ttggcaatcc ctatacgccg ggcgccatcg
      361 agacctgctg cacgacgtcg tggagcgcac tcacactgga tatgctcctg ttgacaggcg
      421 aatcacaggc ggcggatgaa ctcgagtggt ccctgtacaa cagcattctc ggctcgcagc
      481 atcctaccgg ccgctggtgg acatacaaca caccgatgga cggcaagcgt gaagcttcgg
      541 cgcacacgat cgtatttcag tcccgtgccg gcaccccgga attgaattgc tgctccgtca
      601 atgcgccgcg cggtctgggc atgctttccg agtgggcctt gctcagcgat gcatcgggcg
      661 cgctgatcgt caattattac ggtcccatgt ctgccggggt catgctgcag gatgcgggaa
      721 aggtcgctgt gaacgtggaa tccgcctatc ctgcagaagc aagcgcaacg atcctgctcg
      781 ggttggatgc accggcctcc tttccgctgc gactccggat acccgcctgg tcgaagcaca
      841 ccactgtcac cgttgccggc gatgtgcttg acggaatcgg gcccggctcc tacctgacca
      901 tcgagcgcac ctggtcggat aacgaccgga tcgagctggc ctttgacttc tcgatccgga
      961 cgtgggtagg cgacctcgat caggcggagt gcgtgtcttt gttttcgggg ccgatactgc
     1021 ttgccttcga tcagcgcgac aactcatacg attgcgatga actcgcgccc ctcgatttca
     1081 tccatctcag ctatacacgc atagccgata acagccggtt ctcctggatg cccctcttcg
     1141 agttcaaaga cgtgaacggc catgcagtgc ggctgcgcga cttcgccagc gccggtgcaa
     1201 caggcacgga gtaccgctcg tggctgcctg cggcaaacac cccgccgccg cccttccatc
     1261 ttctcgagcc cgccccgcaa gcggcaatac ccgcagggcc attcaagttc acatggactg
     1321 gtccaagaga cggggcagcg caaacgtaca cagtcgagat cagggatact aacggtgcat
     1381 tgacctggca gagcgaaccc acgcgccagc cctggtgcgt ggttcgggcg gagcttacgc
     1441 ccggcacgcc ctatgcctgg cgtgtgctcg cgcagaacac gcacggccgg attttcaatg
     1501 aggagggacc gcggcgcttc gttgtcgacc cggacatgcc aaacctattc gtagatcatc
     1561 cggcgctgct gtcgttcagg aaagacggcc tggcggcaag cggattgctt gacggcgacg
     1621 ggtctccgga atacggatac ctcgatgaag cgtgcggcat aacccgtgga acagaccgga
     1681 acggaaagga cgggggcgcg gttcgctttg ccggggacgg tctcttgcgc tatcgcatgc
     1741 cctatttccc cggagagcac tattcgtttc tcgcctgggt ctgcccggat atactgcccg
     1801 agaccggtct ggcgcaggtg ttcagtgcct ggtcacgcag cggcgacgat ccactgcgcg
     1861 tcgtgatcga aggcgctcaa atctacgcac ggatcgaagg ctctggcggt gcgaatacga
     1921 aaggtcagcc gctcacggcg ggagaatggg tgcacattgc ggcggtaaag gatggcgcgc
     1981 gactgcgcct gtattgcaat ggaacgctcg tggacgatac cgccgctccg gcgaagctcg
     2041 taacgactgc ggaagatttt gcactcggcg ccaacccgca tttttcaggc accgagcact
     2101 ttaccgggcg gatcgatgac ttcgcctttt atgcacgcgc actcagtgaa gcggagattc
     2161 acaaaatgat gaagccctga ccgccgcagc ggtgcctgca tttccggtcg ggattctcat
     2221 acccctgatg gagcggagta ccggtgcggt gacctgcggg ggcaatggac agaatggacg
     2281 gagaagaacg gtcagccgtc ccagggtatt gcggagcggt ggcagggaaa agcctgcccg
     2341 cgctgttcgg gtaatgccag aggacaaccc ctgcatgcgg gcgtttttct acagaaatct
     2401 ttggctgtcc agacaatcag cccgtgaaat tcctgataat cttttaccgt cccggcaaaa
     2461 tttttctcaa tcagcgcctg cagaggatga taggcgatct tttcagaaac gaggccgcat
     2521 cttgaaaaaa ttcgctttgt ataggcgtct accacaaaaa tctttttatg gcaggcataa
     2581 agcaggatat catctgccgt ttccggtccg atccctttga gagaaagcag ctcctctctg
     2641 agttccttca gaggacgctc cccaagtttt ttcatactgc cctgataatg cgtcagcaaa
     2701 tggctgcaga agaggcgcac ccgctccgtt ttctggcgga aataaccgga aggccgcagc
     2761 gcttcttcaa cctcttcgga agcagcttcc gtgagcgctt cgggacttaa ccagtctttt
     2821 gccttcatat tgtccagcgc tttttccaca tttgcccacg ccgtgttctg tgtcagaatg
     2881 gcgcccagcg caatctcaaa aggggtatcg cccggccacc agtgcgtcag tccgaaatga
     2941 cgcttcaggg ctttataaat attttttact gtttcttttc gcgtttccat tgctgcctgc
     3001 tttcaaaaag ccgcagcagc gcaacagcac aaaatgccgc tgcacccact gccgccagaa
     3061 caatcatttt atatcctgat gtcacacgat cgaagagcac cgccagaatc ccggcaccaa
     3121 gcgccacggc atagagggac agaacaacct gcttctggct gagcccgagc gccaggaggc
     3181 gatgatgcag atgctcccga tcgggggtga agaagcggcc cgagcgcgcc cgccgcagcc
     3241 tccgcataaa actgagtacc gtatcgagaa gaggcaccgc cataaccatg acaggaaccg
     3301 acagtgcaat gaccgctgtt cctttctgcc gctcaatcac cgttgaggca cccagcaaaa
     3361 atcccaaaaa aagcgctcct gcatccccca taaaaatgga agccggatga aagttgtaac
     3421 ataagaacgc cagcgtaatc ccgaacagaa acgccatgat cgtgcaggaa aaaacacttc
     3481 cacttcgaaa agacacaaaa aacagcactg cagagcagat acaggtcaat cccgccgcca
     3541 gcccgtccag tccgtccgca aagttgacgg cattcatgat acccgctatc cacagcgccg
     3601 tcaccaccag ggcaaatccg ccgatataaa cagttccgcc gaaaggattg gacagcactt
     3661 cacttttaaa cccccagaaa tagagggaag cacacagcag cagctccaaa ggaagtttaa
     3721 tcttgctcgt cagatcgcag atatcgtcat acacgcccag caatacaatc agaaaaccgc
     3781 ccgccgccag aggataaaac tccggaagca gccgatgcgg tgcagcaaaa gcgaacacga
     3841 taaggagcag cagccaggca agaaaaaagg aaagcccgcc cagatacggc acggcacgtc
     3901 ggtgcacctt gcgttcctgg tcaggctgat ccagagcacc cagtttcaaa gccgctttcc
     3961 tgcacaacgg caccagcata aacaccgcca gaaagcttac cagcgcaaca cttgcataaa
     4021 aaagatcctg ctccctcatg gcgctttccc tccgggaatg gctgcgcaga gaacaggatg
     4081 cccgcgcctg ccgtgatacc actgaggcga ccacacctgc agcgccgagc cgggaacagc
     4141 acctttgact ccgattgcgg ccagactgga ccgaatggca gtcacccgca tttttctttc
     4201 atccatcagg caccggtcga ggccgaaaaa cagaaaggct tcttcaagat catgtgtcac
     4261 tactcccgcc ccgtcatcgt aaacggcaaa aagcccgata caaccttccg gcgcctctgt
     4321 cagaaaagaa aacagacgct gcccttcttc ccggcgctcc cacacatcaa agcatgcagc
     4381 cgccagcagc gccccgctgc ctgcgtccag cagtgcaata tgcatacccc gctcacgcct
     4441 gcccaagtct ttttcgcctg caaagagatg cacgccccgc cgggaatctg
//
LOCUS       DAOPWB010000201         4455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_19669_length_4455_cov_5.627955, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000201 DAOPWB010000000
VERSION     DAOPWB010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19669_length_4455_cov_5.627955"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..500)
                     /gene="cas2"
                     /locus_tag="PKN92_12670"
     CDS             complement(156..500)
                     /gene="cas2"
                     /locus_tag="PKN92_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNZ49689.1"
                     /translation="MSKQLLSLSEYKGMWLLVLFDLPVVEAEERKKYTHFRNALLKDG
                     FMMLQFSVYARFCPSEEASEVHKKRVRFVIPEKGQVRIMSVTDRQFGKMENYVGKTRG
                     KAENEPEQLMLL"
     gene            complement(506..1396)
                     /gene="cas1"
                     /locus_tag="PKN92_12675"
     CDS             complement(506..1396)
                     /gene="cas1"
                     /locus_tag="PKN92_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HNZ49690.1"
                     /translation="MTNRILDLSESPGHVSVWQGLFCLERKGADKITIPFDEIIAIVG
                     SHRQLTFTQAFFARLAQSGGVFVSCDEKSRPVALMLPLEGHYLQTEQFMLQAQVPLPV
                     KKRLWQEVVQAKILAQANTLLELHGEDYGLAAMAARVRSGDSENMEGQAARRYWHHLF
                     QDEPDFRRDRDAEGKNALLNFGYTVLRAVTARAICGAGLHPSMGINHHNRYNTFVLAD
                     DLMEPFRPIIDKATACFQPEQEGMPVLDQKAKRCLYEALYASVSFNGEQRSLFDALTR
                     ICTSLVQVYRGNRRNILLPD"
     gene            complement(1389..>4455)
                     /gene="cas9"
                     /locus_tag="PKN92_12680"
     CDS             complement(1389..>4455)
                     /gene="cas9"
                     /locus_tag="PKN92_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HNZ49691.1"
                     /translation="LEDTLPVELMDMGVRIFEAGTTGDIESGKDESRAIERRAARQRR
                     RQTDRQSRRLRSLALVLQEHGLLPEGELKTGSDRHAFFLQLDGELRNHYAEQGIALNC
                     AAGLREFPYYLRAKALDEKMPLHATGRALYHLAQRRGFLSNRKTDSNDKETGPVKEGI
                     SDLAQKIKQANMRTLGEYLFNIDPVHEKRIRGRWTARSMYEEEFNLIWEAQSRWHPQL
                     YNENIRWNIHKAIFYQRPLKSQAGTVGYCDLEPFKKRAPKALVVSQQFRILQKINDLT
                     VHFADGDVRPLNNDERVSLFEYMNERESVTFKQIRKRLSLPPDTEFNLERGGEKKLPG
                     NVTEARLLKIFGKNWHSFNDAFHHEIISLIMGSEKAESVRKLGKNKYGLDDDQAEKFS
                     RLQFENGYMSLSKKAMTKLLPLMKEGVSFKTAEKKLYDNPRNLCLDFLPPLHTHKNDR
                     FSPQPDAGLDIRNPVVTRSLTELRRVVNNIIAKYGKPLRIHVELARDIKRNREQRKLI
                     STENRDRQRSRESIVAQIKEKLGEHYMPSEQDIEKVLLMRECRCICPYTGRTINMENL
                     LGDHPQFDIEHIIPFSRSLSNSFANKTLCYHEENRNRKRNKTPWEAYGNTDQWDQIVT
                     RVKSFCGPTARIKLERFQLRDLSSLDEMSSQQLNDTRYASKIAARYLGLLYGENAMRH
                     IQTSTGQITAYLRAGWHLNRILSTQGIKNRDDHRHHALDAIVIALTSRSAIQTLSKAA
                     GNAYNAKGRTRTFLNQLEEPWEGFLPEVQEMADNLFISRRINRRVRGRLHEDTNYGYP
                     RTDENGKQWHPIRKVLTETFKDTDIDNIIDPVVQDCVRTHFEANGSNPQKAFADPDNH
                     PRMHDGTVIHKVRCKRTVSTTPIGSGDGTRFVATDTNHHMEIVETQNKNGEIIWEDHV
                     VSLMEAVQRHKAGLPVIKRDHGPGSTFIYSLFKGDTCTITENDETIYCTVIGIAVGEV
                     TLVRMYDARKQVEIRQTKSRIRCNANSLRKKQLRKLLITPLGEVIPAND"
BASE COUNT          983 a         1055 c         1059 g         1358 t
ORIGIN      
        1 catgctgtag ttggtaggga atccccgctc aggtatactt cgacctgtct catcagcgtc
       61 ggtgggtcag ctgtagttgg tagggaatcc ccgctcaggt atactgtctc ccggtgcaag
      121 tccagtattt gcaccgggaa ccttcttttt tctttttata aaagcatgag ttgctcgggc
      181 tcattttctg cttttcccct ggtttttcct acataatttt ccattttgcc aaactgtctg
      241 tcggtaacag acataattct tacctgtcct ttttcaggga taacaaaccg cactcttttt
      301 ttatgcactt cgcttgcttc ttcgctgggg cagaaccggg cgtatacact gaactggagc
      361 atcatgaagc cgtccttgag aagcgcattt cgaaagtggg tatatttttt tctttcttct
      421 gcttccacaa ccggcaaatc gaataagacc aggagccaca tgcctttata ctccgatagt
      481 gacaagagct gtttggacat cgcaattaat ccggtaaaag gatatttctt ctgtttccac
      541 gataaacctg caccagagag gtacagatgc gggtcaatgc atcgaaaagg cttcgttgct
      601 ctccgttgaa agagacagac gcgtacaagg cttcataaag gcagcgttta gccttttgat
      661 ccagaacagg catgccttcc tgctcaggct gaaaacaagc tgttgcttta tcaataatcg
      721 gtctgaaagg ttccataaga tcatcagcga ggacaaatgt gttgtatcgg ttgtgatggt
      781 tgatgcccat ggaggggtgc agcccggctc cgcagatggc acgggcggtg acagcgcgta
      841 aaacggtata gccaaagttt aaaagcgcat ttttcccttc tgcatcacga tccctcctga
      901 aatcgggttc gtcctgaaac aggtgatgcc aatagcggcg tgccgcctgt ccctccatgt
      961 tctcagagtc gccgcttcgt acccttgccg ccatagctgc aagcccgtag tcttctccat
     1021 gcagttcgag gagcgtgttt gcctgcgcca gaatttttgc ctgaacaact tcctgccata
     1081 aacgtttctt tacgggaaga gggacttgag cttgaagcat aaactgttcg gtctggaggt
     1141 aatgcccttc cagaggaagc atcagggcaa caggtcggct cttttcatcg caggaaacaa
     1201 atacaccccc cgattgcgca agccgtgcaa aaaaagcctg ggtaaaagtc agttgcctgt
     1261 gactgcctac tatggcaata atttcatcaa agggaatcgt gattttatct gcgccttttc
     1321 gctccaggca gaaaagcccc tgccagacac tgacatgtcc cggcgattca gataaatcaa
     1381 gaatacgatt agtcattcgc aggtattact tctcccagcg gggttattag gagttttctt
     1441 aactgttttt ttctaagact gtttgcatta catctaatcc tgcttttggt ttgccgtatt
     1501 tctacctgct tccgtgcgtc atacatacga actagtgtaa cttcacctac ggcaattcca
     1561 atgacagtgc aataaattgt ttcgtcgttt tcagtgattg tacaggtatc gcctttaaac
     1621 aaagagtaaa taaaggtgga tcccggacca tgatcccgtt taataacagg taatcctgcc
     1681 ttatgccgct gtacagcttc catcaggctg actacgtggt cttcccatat aatttcaccg
     1741 tttttgtttt gtgtttcaac aatttccata tgatggtttg tatcggtggc aacaaatctt
     1801 gtcccatcgc ccgatccgat gggagttgtc gagacagttc ttttgcagcg tactttgtga
     1861 atcacagtgc catcgtgcat tcgagggtgg ttgtccggat cggcaaaagc tttctgcgga
     1921 ttggagccgt ttgcctcaaa atgcgtgcgt acacagtctt gaacgaccgg gtctataata
     1981 ttgtcaatgt ctgtatcttt aaatgtctct gtcagcactt ttctgatggg atgccattgt
     2041 tttccgtttt cgtccgtgcg tggatatccg taattcgtat cctcgtgaag ccttccgcgc
     2101 acccgtctgt taatccttct tgaaataaat aggttatccg ccatttcctg tacttcaggc
     2161 aggaagcctt cccagggttc ttcgagctga tttaaaaatg ttcttgttct tccttttgca
     2221 ttataagcgt ttcctgccgc tttgctgagt gtctggatgg cagaacggct tgtaagggca
     2281 atgacaatag catccagcgc atggtgcctg tggtcatctc tgtttttgat cccttgcgtg
     2341 ctgagtattc tgttcaaatg ccagccggct cttaaatatg ctgtaatttg tccggtgctg
     2401 gtctgaatgt ggcgcatggc attttcgccg tacagaaggc cgagataacg ggcggcaatc
     2461 tttgaggcat agcgtgtatc gttcagctgc tgcgaactca tttcgtccag cgaactcaaa
     2521 tcgcgcaact gaaagcgttc caacttgata cgggctgttg gaccgcagaa acttttaact
     2581 cgagtgacaa tctgatccca ctggtctgtg tttccgtatg cctcccaggg cgttttgttt
     2641 ctttttctgt ttctgttttc ctcgtggtag cagagggtct tattggcaaa actgttggag
     2701 aggcttctgc tgaaaggaat aatatgttcg atatcaaact gcggatggtc gcccagcagg
     2761 ttttccatat tgattgtgcg acctgtataa gggcatatgc agcggcattc ccgcataaga
     2821 aggacttttt cgatatcctg ctcgctgggc atgtaatgtt cgcctaattt ttctttaatt
     2881 tgagccacaa tactttctct ggatctttgt ctgtctctgt tctcggtgga aatgagtttt
     2941 cgctgttccc gatttctttt aatgtctctt gcgagttcga catgaatcct gagaggtttt
     3001 ccgtatttgg caataatgtt attaaccacc cgccgcagtt cggtgaggct tcgggtcaca
     3061 acagggttcc ttatgtcgag gcctgcgtca ggctgcgggc tgaaccggtc attcttgtgc
     3121 gtgtgcaagg gcgggagaaa atccaggcac agattgcggg gattgtcgta aagttttttc
     3181 tcggctgttt taaaagagac cccttccttc ataaggggca gtagtttggt cattgctttt
     3241 tttgagagag acatatagcc attttcaaac tgcagccggg aaaacttttc agcctggtca
     3301 tcgtccagac catatttatt tttgccgagt tttctgacag actccgcttt ttcactgccc
     3361 ataataaggc tgataatctc atggtgaaac gcatcattga aactatgcca gttttttccg
     3421 aagattttta gcagccgtgc ctcggtgacg tttcccggaa gttttttttc tcctccacgt
     3481 tccagattaa attctgtgtc cggaggaagg gaaaggcgtt ttcttatctg cttaaaagtt
     3541 acggattccc gttcattcat atattcaaaa agagacaccc gctcatcgtt atttaaaggt
     3601 ctgacgtcgc catctgcaaa atgaactgtc aggtcattaa ttttttgcag tatccggaat
     3661 tgctggctta cgacaagagc tttgggtgct ctttttttga atggttccag atcacagtag
     3721 cctactgtgc ccgcctgact ttttaacgga cgttgataaa aaatggcctt gtgtatgttc
     3781 caacggatgt tttcattgta taactgagga tgccaccggc tttgtgcttc ccagatgagg
     3841 ttaaattctt cttcgtacat ggagcgggct gtccagcggc cacgtatgcg tttttcgtgc
     3901 accgggtcaa tattgaagag atattcgccc agtgttcgca tatttgcctg tttgattttt
     3961 tgagcaagat cggagatgcc ttctttgaca ggccctgttt ctttgtcgtt gctgtccgtt
     4021 ttcctgttgc ttaaaaaacc ccggcgttgt gcgagatgat agagtgctct gcctgtagca
     4081 tgcagcggca tcttttcgtc cagtgccttt gcgcgcaagt aatagggaaa ctcacgaaga
     4141 ccagcagcac agtttagtgc aatgccctgc tcagcatagt ggttgcgaag ttcgccgtcc
     4201 aattgaagga aaaaagcatg gcggtcactg ccggttttta attctccttc cggcaagaga
     4261 ccatgttcct gaagtaccaa tgcaagggag cggagtcgcc gtgactgcct gtcggtctgg
     4321 cgtcgccgct gccgcgcagc tcgtcgctca atggcccggg attcgtcttt tcctgactct
     4381 atatctccgg ttgtgccagc ttcaaagatg cgaaccccca tgtccatcag ctcgacaggg
     4441 agggtgtcct caagg
//
LOCUS       DAOPWB010000202         4299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_20634_length_4299_cov_6.010368, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000202 DAOPWB010000000
VERSION     DAOPWB010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20634_length_4299_cov_6.010368"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="rhaT"
                     /locus_tag="PKN92_12685"
     CDS             <1..1041
                     /gene="rhaT"
                     /locus_tag="PKN92_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HNZ49692.1"
                     /translation="RVRLWSWETYWLVGGFFSWIIAPFILASILVPDLIGVIRSAPSS
                     SLLWAYFWGAMWGLGGLTFGLTMRYLGIALGMAIALGYCAAFGTLMPPIFDGSITTLI
                     LHAPGQIVLFGVLICLTGIGVSGMAGMSKEKEMPEEEKKAAIKEFNFPKGLMVATFSG
                     VLSASMAYGLAAGKPIASIALEHLTDAGKADLWQNLPSLVVVLWGGFTTNFLWCLYLN
                     ITNGSGHEYFATRTKAAEPEKSESVPMLNNYVFSAMAGVTWYMQFFFYSMGETKMGKY
                     SFSSWTLHMASIIIFSTLWGIFLREWHGTSSKTKVLVAVGLAVLILSTLIVGYGNYLN
                     TLPQYAAAAATN"
     gene            complement(1169..1933)
                     /locus_tag="PKN92_12690"
     CDS             complement(1169..1933)
                     /locus_tag="PKN92_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49693.1"
                     /translation="MKALPSEDKQLEKQIHALLREALGRRYELAHLRKYSEFDPVPDN
                     TLETLRLFGLRHIYPDWEQRAFQIRIFQKTQTLLQSPTRLMALSRTALLSLFRFSAQV
                     PGAVKAGMQVISAFDATRKLEEKIFSHARRLNLEDRIEKEGMGGLAPALAALVPEDYE
                     TYLQGLTALVQILIQPRLLDAGESILGEMARAMRKRPGLFDEEERQGAQYAAEALAEG
                     RKIFNSLPTDVIAGAAEGIPHLELDWLAEASQKPRS"
     gene            complement(1908..2120)
                     /locus_tag="PKN92_12695"
     CDS             complement(1908..2120)
                     /locus_tag="PKN92_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNZ49694.1"
                     /translation="MRTIANQTGITGWVRNLPDGAVEALIQGDDAALAAMKAWCREGP
                     AFARVDQVQETLRTGEAAHESFAIRG"
     gene            complement(2173..3540)
                     /locus_tag="PKN92_12700"
     CDS             complement(2173..3540)
                     /locus_tag="PKN92_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49695.1"
                     /translation="MKRSLFLCTVVVFAGLFSLTAISAPVPITGTSQSVSLSDLVGTR
                     TMLTVVLKDGDAKDSNLKLAEVRDDRLVFLSTTGEIVPYLLDSIASIEVQDGVVQQRR
                     APDLESQVLRAEHQRIVDRAWSRIKDIFQQSDDNQPLKMEAAMLLAMSGEENAHEYLR
                     QLAGSNDLVLQLQAAGMLYLIGDVVPENLIRQGLESGKRDARIMAASLSGLAGYKDAV
                     PTLRPLFMDRSVQYSAPAARALARLDDHTILDRLQAMLFELQQEKGEAAIFALTKLQD
                     ASVIEGLKFRLLEADGSNRLRIARVLFNLGDPTGIDELQHIFKTYPTLMPEVALMLAA
                     NGYWDAIQFLQTRLARREDPTESNLAFRARNAQALLASGDPSAMAVFQELLRSDKEAV
                     TSLVFSLMTELGQPRLITLLQPSIENSNPAYALDACKAVISLALPSFRTRLLEYRIEF
                     PEITH"
     gene            complement(3772..>4299)
                     /locus_tag="PKN92_12705"
     CDS             complement(3772..>4299)
                     /locus_tag="PKN92_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49696.1"
                     /translation="QAIVGEKKVQGKKGYYLETEIVPDVGFPIIYKMLLTGPATDKNN
                     VHEIIVREGFGAPETIALDNLEGAEDEKSPEAARTSLGTETVKTPAGDIQAEHFSIEQ
                     GGLKTEVWISDTVRPMGIVRMISSDGELLLSRSGQGGADGESAMNRTPPEEIRPDVKV
                     RVESGPTRNFSGKAQ"
BASE COUNT          918 a         1181 c         1160 g         1040 t
ORIGIN      
        1 cgtgtacgtc tttggtcctg ggaaacctac tggcttgtag gcggattttt ctcctggatt
       61 atcgcgcctt tcatcctcgc cagtattctg gtgccggatc tgatcggcgt gatcaggagt
      121 gctccctcat ccagcctctt gtgggcatac ttctggggcg ccatgtgggg gctgggcggc
      181 ctgaccttcg ggttgaccat gcgttacctg ggcatcgccc ttggcatggc aattgccctg
      241 gggtattgcg ccgctttcgg tacgctgatg ccgcccattt ttgacggctc cattacgaca
      301 ctgatccttc atgcgcccgg gcagattgtg ctgttcggcg tgcttatttg tctcaccggc
      361 atcggtgtga gcggtatggc gggcatgtcc aaggaaaagg aaatgcctga agaggaaaag
      421 aaagcggcga tcaaggaatt taattttccc aaaggactga tggttgccac cttctccggt
      481 gtactgagtg cttccatggc atatggactt gctgctggaa agcccatcgc aagcatcgct
      541 ctggagcatc tcaccgacgc aggaaaagcc gatctctggc agaatctgcc gtcactggtt
      601 gtggtgctct ggggcggatt taccaccaac ttcctttggt gcctttatct gaatattacc
      661 aatggcagcg gacacgaata ctttgccacc agaaccaaag ccgcggaacc agaaaaatcg
      721 gaatctgtcc ccatgctgaa taactatgtg ttttccgcca tggcgggcgt gacctggtac
      781 atgcagtttt tcttctacag catgggcgaa acaaaaatgg gcaaatacag cttttccagc
      841 tggacgctgc acatggcgtc tattatcatc ttcagcacct tgtggggcat tttcctcaga
      901 gaatggcatg gcacgagcag taagacaaaa gtgctcgtcg ccgttgggct tgcggtactg
      961 attctctcca cattgattgt gggctacgga aactatctga atacgctgcc ccagtatgcg
     1021 gcagcagcgg caaccaattg acggttttgt tttcggcgga tacgtgcagc agcgtcagag
     1081 agcgaaaact ctttggcgct gctttttttg aatgctccgc ccgaagtgca tactcccgga
     1141 atttgcttca ccctttaaag acggagactc aggatcgggg tttctgcgag gcttccgcga
     1201 gccagtccaa ttcgagatgt gggattcctt ctgctgcacc ggctataaca tcagtcggaa
     1261 gggaattaaa tattttccgt ccttccgcaa gcgcttccgc cgcatactgt gcgccctgac
     1321 gctcttcttc atcgaataag ccggggcgtt tccgcatggc acgcgccatt tcccccagaa
     1381 tggattctcc agcatccaga aggcgcggct gaatcaggat ctgtaccagc gctgtcaggc
     1441 cctgcaggta ggtctcataa tcttcaggca ccaaagctgc caaagccggc gccagaccgc
     1501 ccatcccttc cttctcgatg cgatcttcca gattcagccg gcgggcatgg gaaaaaatct
     1561 tttcttccag ttttcgggtg gcgtcaaaag cggaaatcac ttgcatccct gccttgacgg
     1621 caccgggcac ctgtgcggag aagcggaaga gagacagaag agccgttcgg ctcaatgcca
     1681 tcagccgtgt cggcgactgg agcagagtct gggtcttttg gaaaatacgg atttgaaaag
     1741 cacgctgttc ccaatccggg tagatgtggc gaagaccgaa aaggcgcagc gtttccagtg
     1801 tgttgtccgg aacagggtca aattcggagt acttgcgaag atgcgccagc tcgtatcgcc
     1861 tgcccagagc ctcgcgaagc aacgcatgta tttgcttttc gagctgttta tcctctgatg
     1921 gcaaagcttt catgcgctgc ttctcctgta cgaagggtct cctgtacctg atctacccgg
     1981 gcaaaagcag gaccctcgcg gcaccaggct ttcatggcgg caagtgcggc atcatctccc
     2041 tgaatcagcg cctccacagc gccatccggc agattgcgca cccagcccgt gatccctgtc
     2101 tggttagcga ttgtgcgcat ggtgtagcgg aagccgacgc cctgtacacg tcccgatatg
     2161 cgcagcagca gactagtgtg tgatttcggg gaactcaatg cggtattcca gaaggcgtgt
     2221 ccggaaagag gggagtgcca gagaaatgac ggccttgcag gcgtcaagcg cataggccgg
     2281 gttggagttt tcaatacttg gctgaagaag cgtaatcagc ctgggctgcc ccaattcagt
     2341 catcagggag aagacgaggc tggtgaccgc ttctttgtct gagcgcagaa gttcctgaaa
     2401 tacagccatg gcggaagggt cgccgctggc gaggagtgcc tgggcattgc gggcacggaa
     2461 agccagattg ctttcggtgg gatcttcacg ccgtgcaagc cgtgtctgca gaaactgtat
     2521 ggcatcccaa tacccgtttg ccgccagcat cagtgccact tccggcatga gcgtgggata
     2581 ggtcttgaaa atatgctgca gttcgtcaat tcctgtcgga tcgccaagat tgaaaagaac
     2641 gcgcgcaata cgcagccggt tggagccgtc ggcttccagc agcctgaatt tcagtccttc
     2701 aatgaccgag gcatcctgca gctttgtcaa tgcgaagatg gcggcttctc ctttttcctg
     2761 ctgcagttcg aaaagcatgg cttgtaaacg atcgagaatt gtgtgatcat cgagccgtgc
     2821 cagagcccgg gcggccgggg cggaatactg aaccgaacgg tccataaaca ggggacgcaa
     2881 tgtaggcacg gcatctttat agccagccag cccggaaagg ctcgccgcca taatgcgcgc
     2941 atccctcttt ccgctttcca atccctggcg gatcaggttt tcggggacca catcgccgat
     3001 caggtacagc atacccgccg cctgcagctg aagaaccagg tcgttggagc ccgccagctg
     3061 acgcaggtat tcatgtgcat tttcctcgcc ggacatggca aggagcatcg ccgcttccat
     3121 tttgaggggc tgattatcgt cgctctgttg aaaaatatct tttatccgtg accaggcacg
     3181 atccacgatg cgctgatgtt cggcacgcag tacctgagat tccagatccg gggcgcgcct
     3241 ctgctgcacg acaccgtcct gcacttcaat ggaggcgatg gagtccagca gataagggac
     3301 gatttctccg gtagtgctca aaaaaaccaa acggtcgtcc cgcacttccg ccagtttcag
     3361 attactgtct tttgcgtccc catctttcag aaccacggtc agcatggtcc gcgttcccac
     3421 cagatcagac aggctgacgg actgggaggt gcctgtgatc gggacaggtg ccgagatggc
     3481 agtcagactg aacagtcccg caaaaaccac tacggtacac agaaacagtg atctcttcat
     3541 ctttgtcaat tcctttttca tgtctcttgc aaaccgggca gtagcacggt gctctgccgg
     3601 gcattcgggg agtgttcacg cacaattata atgctcacat tatgcataag agggcacatg
     3661 gcttcaaaac agccttcaca cccgatcagc ccgtaaaaag aggctttccg gaaaagcaca
     3721 gggacttttc ggaagatacc gccgcttttc agtagtttct ccgttggagg tctattgggc
     3781 ttttcctgaa aaattccggg ttgggcccga ttcaacacgt acttttacat ccggacggat
     3841 ttcctcgggt ggtgtccgat tcatagcact ctcgccgtct gcgccacctt gtccgctgcg
     3901 gctgagcagc aattcgccgt cgctgctgat catccgtaca atacccatgg ggcggacggt
     3961 gtcgctgatc catacttccg tcttgagacc gccctgttca atggaaaaat gttccgcctg
     4021 aatatcccct gccggagttt ttactgtttc cgttcccagt gaagtgcgtg cagcctcggg
     4081 agatttttca tcctctgcac cctccagatt gtctaatgca atcgtttccg gggcgccaaa
     4141 gccttcccgc acaataattt catgaacgtt atttttatcg gtggcaggtc cggtcagcag
     4201 cattttataa atgatgggaa agccaacgtc cggcacaatt tctgtttcca gatagtagcc
     4261 tttttttccc tgtacttttt tctctcccac gattgcctg
//
LOCUS       DAOPWB010000203         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_20761_length_4280_cov_4.708639, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000203 DAOPWB010000000
VERSION     DAOPWB010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20761_length_4280_cov_4.708639"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            397..3195
                     /locus_tag="PKN92_12710"
     CDS             397..3195
                     /locus_tag="PKN92_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNZ49697.1"
                     /translation="MNAFIRTLMAFTGFGFCICICALLTGCPEDSTARYGILQVVNDR
                     TPTVVELYISPSDDDDLGDNLLDSRLPKGYLFEIYLSPGRWDIFTVDTNNEIRYALNV
                     SVLKGQTRAVYLSGMTVYHDEEEEGESPDPHEGESGSDLSTGARTLQGKKPFAASLPD
                     GTRLTLPAINLSGTLTSLTLSKEYSSPDLSATDIEPVSAVRIVSFDSFDPVRMGGRFA
                     DFTPSLTFPAADVAGQDPTTLTAVRVSDLLLGDDDLPGHISYLPVTKNAKGDYEITDF
                     YFPDSIMSDLAQKKNVQKALRRPREIRYVLGTFKGSYNWKAQPQLERFFAAEDLPHKR
                     TVWGNLTAEERNEEENKQIVNMVVLVHGHNEEEKEYGLNASAENPWYFAYKQDVWSPL
                     YDYVLRTKEDILDCTAFYEFIYPTYKPIFTDTPGIPGGRLDQAFAREVNAEVESLLSM
                     LPAGQELRLYIVAHSMGGLVARAGIQLFSANSHTAFQKLVTWGSPHMGGALVSMRYVL
                     GAPGGIYRVGSEAYAATQPLGNIDNTLFALRRSIDQMQADTPGTRDLRYANSHTTTPY
                     NLVLDQLFSMDISPAGDAALWHKYDLRSGTEIYNENLRLLNAEDSYRLSSKYHALFGV
                     TSKRAQVELTWYYRPYVEGNEIAKGALVIPWLIEKAKDSYEGYTRGASDGAVNIASMI
                     GAGVMGTRYSLGDVDHEEYYGAPTKDRFTRLDLAEKTLTSTFNALDIRPCTKTKLQIQ
                     SIAPSQGIIGCSVTIQGSGFQNAPRDSAGYIILNSDFFKTHKVTVGGVAAQLKDWNDN
                     SITITVPEFSQKTGDVVVHAGKEKSNSKSFTSVLDVMIEKKFLRQEPTEAEIAALIAQ
                     GIIPDPPAYYYEWKAVVTSPAVMPLSFKWFENGQEYGTTAAVTVKSSTPGKEMKVEVK
                     DSVGRSGTKSDLTGIADL"
     gene            complement(3212..>4280)
                     /locus_tag="PKN92_12715"
     CDS             complement(3212..>4280)
                     /locus_tag="PKN92_12715"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNZ49698.1"
                     /translation="LHCLDRDGTLLDSDKSVWAQGRMAWLLLTMYNQVEKRTQWLTWA
                     ESGIQFLEKHCVDQDGRFFFHVTREGLPIRKRRYAFSEAFAAIAQAAYARATGSDRAA
                     ERAIDLFERFVFLNFTPGAVAAKFTDTRPLTGLASRMITLVTARELTKNLGEQPGLNG
                     WMERVIGEIISLFVKPEERCVLESVAPDGSISDHFDGRMLNPGHAIEAAWFIMEEGRI
                     RKDKALVEEGCRMLDWMWQRGWDQEYGGLYYFRDLYNRPVQEYWHDMKFWWPHNEALI
                     ATLLAWRLTGEESYAEKHLQLLDWCLEHFADPEHGEWYGYLHRDGTVSVPLKGNLWKS
                     FFHHPRCLWKCWNLCRENISV"
BASE COUNT         1054 a         1138 c         1114 g          974 t
ORIGIN      
        1 tgcagatcaa gacgtccata ccctgattaa gattcctgcc ttcgcaggaa tgacgtggtg
       61 tgcaggaatg acggggtgtg caggaatggc ggtttcataa ggcggaagtg ctagcccgac
      121 tgcagggatc ttaccccgga gcaagatttc tgcaacatcg ttatttgtct cagccacctg
      181 gtattcattc gtgttcattc gtggttccgt ccccggcccg gtgttgtcat tccgtgcacg
      241 cagtgccgta gcagcaaaaa cattctcttt tataactatt tctttttttg ggacgtttta
      301 cctatactta tgagacaaac aaacaaaagt caaaaaatcg ccaggcgctt actgtgaaag
      361 caatgctgtt gccagcactg caacaggaga attcgcatga atgccttcat cagaacactg
      421 atggccttta cagggtttgg tttttgtatt tgcatctgtg cgctgctgac aggctgcccg
      481 gaagacagca cggcgcggta tggcattctg caggtggtca atgaccgtac gccaactgta
      541 gtggaactgt atatcagtcc ctccgatgat gatgacctcg gcgacaatct gctggactcc
      601 cgtctgccca aagggtattt atttgaaatc tatctgtcgc cgggccgctg ggacattttc
      661 acggtagata cgaacaacga aatccggtat gccctcaacg tgtcggtttt aaaaggacag
      721 acccgcgcgg tgtatctgtc gggcatgacc gtttatcatg acgaagagga ggaaggcgaa
      781 tctcccgatc cgcacgaagg tgaatcggga agcgatcttt ccaccggtgc ccgcaccctt
      841 cagggaaaga aaccctttgc cgcctctctt cccgacggaa ccagactgac actgccggcg
      901 atcaatcttt ccggcacact cacctcactg actctgtcga aagaatacag ttctcctgat
      961 ctcagtgcca ccgatattga acccgtaagt gccgtgcgca tcgtatcctt tgattccttt
     1021 gacccggtcc ggatgggcgg cagattcgcc gatttcacgc cctcgctgac ctttcccgct
     1081 gcggatgttg ccgggcagga tcctaccacg ctgacggcag tccgtgtatc cgatctgctg
     1141 cttggcgatg acgacctgcc gggacatatc agctatcttc cggttactaa aaatgcaaag
     1201 ggcgattatg aaatcacgga tttttatttt cctgattcca tcatgtccga tctggcacag
     1261 aaaaagaatg tgcaaaaagc ccttcgccgt ccccgggaaa tccgctatgt gctgggcact
     1321 tttaaaggga gttacaactg gaaggcacag cctcagctgg agcgtttctt tgctgctgag
     1381 gatcttcccc ataaaagaac tgtatgggga aatctgacgg ctgaagaaag aaacgaggaa
     1441 gagaacaagc agattgtcaa tatggtagtg cttgttcacg ggcataatga agaggaaaaa
     1501 gaatacgggc tgaatgcatc ggcggaaaat ccctggtatt tcgcatacaa gcaggatgtg
     1561 tggagtccgc tctatgacta tgtgctgcgc acaaaagagg acatactgga ctgcaccgct
     1621 ttttacgagt ttatctatcc cacctataag cccattttca cggatacccc cggcatcccc
     1681 ggagggcgac tggatcaggc ttttgcccgg gaggtcaatg ccgaagttga atcactgctg
     1741 tcgatgctgc ctgccggaca ggagctgcgc ctgtatatcg tcgcccactc catgggcgga
     1801 cttgtcgccc gggcaggcat acagcttttc agtgcgaaca gccatactgc ctttcagaaa
     1861 ctggtcacct ggggcagccc tcacatgggc ggtgccctcg taagcatgcg ctatgtactg
     1921 ggcgctcccg gcggaattta ccgggtaggc tcggaagcct atgccgctac gcagcctctg
     1981 ggcaatattg acaacacgct tttcgctttg agaagaagca ttgatcagat gcaggcagat
     2041 acgccaggca ccagagatct gcgctatgcc aacagccaca ccaccacgcc ttataatctg
     2101 gtgctggatc agcttttttc catggacatc tcgcctgcag gcgatgcggc actgtggcat
     2161 aaatatgatc tgcgttcggg cacggaaatt tataacgaaa acctgcgtct gctgaacgca
     2221 gaggattcgt accggctgag cagtaaatat catgcccttt tcggggtcac gagcaagcgc
     2281 gcacaagttg aacttacctg gtattaccgg ccctatgtgg agggcaacga gattgccaaa
     2341 ggcgctctgg tgatcccctg gctcatcgaa aaagccaaag acagctatga agggtacacc
     2401 cggggtgcca gcgacggtgc cgtcaatatt gccagcatga tcggggcagg cgttatgggc
     2461 acccgctatt cgctgggcga tgttgatcac gaggaatatt acggtgctcc gacaaaggac
     2521 cgcttcaccc gtctggatct tgcggaaaaa acactcacca gcacttttaa tgcccttgat
     2581 atccgaccgt gcacaaagac aaaacttcag attcagtcca ttgccccgtc gcaaggaatc
     2641 atcggctgct cagtcaccat ccagggaagc ggtttccaaa atgcgcccag agacagtgcc
     2701 ggttacatta ttttgaatag tgattttttc aaaacgcata aagtaactgt cggcggtgtg
     2761 gcggcacaac ttaaggactg gaatgacaat tccatcacga tcactgtacc cgaattcagt
     2821 cagaaaaccg gggatgtcgt ggtacatgca ggcaaagaaa aaagcaacag caaaagcttt
     2881 acctctgtgc ttgacgtgat gatagagaag aaatttttac ggcaggagcc cacagaggcc
     2941 gagattgcgg cacttatcgc acagggcatc ataccggatc cgccggctta ttattatgaa
     3001 tggaaagcag tagtcacttc gcccgccgtc atgcctttaa gttttaaatg gtttgaaaac
     3061 ggacaggaat acgggacgac tgcggcggta acagtaaaaa gcagcacccc tggcaaggag
     3121 atgaaagtgg aggtgaaaga cagcgtcggt cgttctggaa cgaaatcaga cttgacaggc
     3181 atagcggatc tttaagccgg ggcaggcagg attacactga aatattttcc cggcagagat
     3241 tccagcattt ccacagacag cgggggtgat ggaaaaaact tttccagaga ttccccttca
     3301 gcggaacaga aaccgttccg tcgcggtgca gataaccgta ccattcgccg tgttccggat
     3361 cggcaaaatg ctccaggcac cagtcaagca gctgcaggtg cttttcggca taagattcct
     3421 caccggtcag acgccaggcg agcagtgtgg cgatgagtgc ttcattatga ggccaccaga
     3481 atttcatatc atgccagtat tcctgaacag gtctgttgta caggtcccgg aaataataga
     3541 ggccgccgta ttcctgatcc cagccccgct gccacatcca gtcgagcatg cggcatcctt
     3601 cttcgaccag tgctttatct ttacgtatac gcccttcttc cataatgaac cacgccgctt
     3661 caatagcatg gccgggattc agcatacgtc catcaaagtg gtcagaaatg cttccgtcgg
     3721 gggcaacgct ttccagtacg cagcgctctt cgggcttgac aaaaagcgaa ataatttctc
     3781 cgatgacccg ctccatccag ccgttcaacc cgggctgttc ccccagattt ttcgtcagtt
     3841 cccgggcagt gaccagcgta atcatccggg aagccagacc ggtgagcgga cgggtgtcgg
     3901 tgaatttggc ggccacggcg ccgggtgtaa aattaaggaa gacaaaacgc tcgaacaggt
     3961 caatagcgcg ctccgctgcc cgatcacttc cggtcgcacg ggcatatgcc gcctgcgcaa
     4021 tcgcagcaaa ggcttcgctg aaggcataac gtcgtttccg gatcgggagc ccctcccggg
     4081 tcacatgaaa aaagaatctt ccgtcctgat caacgcaatg tttttccaga aactgaatgc
     4141 ctgattccgc ccaggtaagc cattgcgtgc gtttctccac ctggttatac atcgtgagca
     4201 gcaaccacgc catacgcccc tgtgcccaga ccgatttgtc agagtcgagg agggtgccgt
     4261 cccgatcgag gcaatgcaga
//
LOCUS       DAOPWB010000204         4270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_20832_length_4270_cov_3.897746, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000204 DAOPWB010000000
VERSION     DAOPWB010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20832_length_4270_cov_3.897746"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2214
                     /locus_tag="PKN92_12720"
     CDS             <1..2214
                     /locus_tag="PKN92_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNZ49699.1"
                     /translation="VTPSNEFQEQARQFRIKEIWSMEALEKAREAIPLFPGLIDVESC
                     TDWDIGMPMDEAALKDKDNEDYWNQWRQTPKLWVNLSAGQKMWGSRFGQYTALHFPAG
                     WGNAPALEKALLNKISPEMLGIRFNPVREEALHSVDQALDLGQLFLGMSFFLIFSAVL
                     LSVLLFTFSLQQRASEMGTLLALGFPPSRIWGIFSIEAALLAVAGATAGMLAGAGYAA
                     LLLQGLRHAWAGAVAGTMILFHLKLKTLFSGFFAGAGIPLLAVCWTVWRQCRRPVREL
                     LHRDFSQKKALTAAGRPGRLAHGLAFGGLLAGLIAVAAVFVIRPAATAPFFFATGTWL
                     LGFGIYAWYLFLRSFQMEKEGGSLSLNKLALQQLTRRPGRNTSAAALLACGVFIAISV
                     ISMQEDLGKHAAERSSGTGGFALFGETTAALTEAPKLEGIDAVALRLRQGDDAGCLNL
                     TRAAVPGIYGVDPAVMKKRGAFDKDADGASVWSLLEQESPDGAIPALVGDMDTAMWGL
                     KAKTHPEKGDVLYYSDDEGKEISVRLVGALPMRLSVFQGSILISLDNFTRIFPSESGF
                     RAFLFDSQNENTEETIRRLHRQEEKSGMQVETTLQRLEHFYAVERSYLSLFLVLGVLG
                     VTLGALGIGVITARSRIERRAEWAMLQVLGYEKRHLLRLLVVENAAILLVAAILGGGA
                     SLTALLPSVLLSSTTLPLLLQFIGFLSMLAGGALSVALALLVTRSQSLLLDLRRE"
     gene            2498..3742
                     /locus_tag="PKN92_12725"
     CDS             2498..3742
                     /locus_tag="PKN92_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ49700.1"
                     /translation="MKKYIFFMLCFCAVPLLFGTGCTVPAEGTVDITLAAAEPPITAT
                     PVQAVLPHRGTVSSWFETTSRVEAEVRVDLLSKGTGQCEKVHVNVGDRVQQGQVVAEL
                     ERRELEAQARQTRVGVQQAKTAYEIAERSFNEGIGASAERDNARFSYEQAKAAAELID
                     LQISNQTLRAPISGIVTRKMVQEGMVVAAGMPVVSIVDPDSFVLPIEVPEKELGRLKE
                     GQEAQVSIDAFPDRSFAAHVVRIDPSINPLSGTVRMLLSFLAEDKPLLREAAFARVKL
                     AMETKGGVLLVPRDAVMEEEGRKYVFALEKEDESQYKPEQLEELRGNALYVAKRIDIT
                     VGLEQNENIEVLTGLNPDNMVAIMGHHSLKDGAFVILTDMQKELAARSALTLEEALLA
                     AKKAKNLMVGSGQDGRSSVSSL"
     gene            3928..>4270
                     /locus_tag="PKN92_12730"
     CDS             3928..>4270
                     /locus_tag="PKN92_12730"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ49701.1"
                     /translation="MADTSSGSTPHYRFAIRRPVSTAMLFLTLIVFGWRSYQQLPVNL
                     MPDISYPSLTVRTEYEGAAPEDVEMLVTRPLEELLSIVSSMVEISSVSSPGLSEITLE
                     FDWSADIASAQQ"
BASE COUNT          941 a         1021 c         1210 g         1098 t
ORIGIN      
        1 gtgacgccct ccaatgagtt tcaggagcag gcgcggcagt tccgcataaa agaaatctgg
       61 tctatggaag cgctggaaaa agcgcgggaa gcgattcctc tgtttccggg gcttatcgat
      121 gtggaaagct gcacggactg ggatataggc atgcccatgg atgaggcggc tctgaaggac
      181 aaggataacg aggattattg gaaccaatgg cgccaaacgc cgaagctctg ggtgaattta
      241 tctgccggac agaaaatgtg gggcagccgc ttcggtcaat atacggcgct tcatttcccc
      301 gcaggctggg ggaatgctcc tgcgctggaa aaagcccttc tgaataagat ttcaccggaa
      361 atgctcggaa ttcgttttaa tccggttcgg gaagaggcgc ttcattcagt tgaccaggct
      421 ctggatctcg ggcagctgtt tctgggaatg agctttttcc tcattttttc agcagttctt
      481 ttgtctgttc tgctgtttac gttcagcctg cagcagcgcg catctgaaat gggaactctc
      541 cttgccctgg gatttccgcc ttcccggatc tggggcattt tcagtattga ggcggcattg
      601 cttgccgtgg caggggcaac agcaggaatg ctggctggag cgggatatgc agccctgctt
      661 cttcaggggc tcaggcacgc ctgggcaggt gcggtcgcag gaacaatgat tctgtttcat
      721 ctgaaactga aaacgctttt cagcggcttc tttgcaggag ccggtattcc attactggcg
      781 gtgtgctgga ctgtgtggcg tcagtgccgg cgtcctgtgc gcgaactgct gcaccgggat
      841 ttttcccaga aaaaagcctt aacagctgcc gggcgtccgg gcaggctggc gcacggtctt
      901 gctttcggcg gtcttttggc gggactgata gcggtcgctg ctgtttttgt tattcggccg
      961 gctgccacgg cacctttctt tttcgcaaca ggcacctggc ttctgggctt cgggatttac
     1021 gcctggtatc tttttctgcg gtctttccag atggaaaaag aaggcgggag cctgtctctg
     1081 aacaaacttg cactacagca attgacccgt cgtcccggca gaaataccag tgcggcagca
     1141 ctcctcgcgt gtggtgtttt tattgccatt tccgtaatat ccatgcagga agacctcggg
     1201 aaacatgcgg cggagcgaag ttccggcaca ggcgggtttg cgctgttcgg agaaacaaca
     1261 gcagccctga ccgaagcccc gaaactggaa gggatcgatg ctgtagcgct gcggctccgg
     1321 cagggagatg atgccggctg cctgaatctg acccgggcgg ctgttcccgg tatttacggg
     1381 gttgatccgg cggtcatgaa aaaaagagga gcctttgaca aagacgctga cggtgcatcc
     1441 gtctggtcat tactcgaaca ggaatcgccg gatggcgcca ttcctgccct tgtcggggac
     1501 atggatacgg caatgtgggg attaaaggct aaaacacatc ctgaaaaagg ggatgttttg
     1561 tactattctg atgacgaagg taaggaaatt tctgtacgcc ttgtcggagc gctgcccatg
     1621 cgtctttccg tttttcaggg gagtattctg atatctctgg ataactttac gagaattttt
     1681 ccatcggaaa gcggatttcg cgccttcctt tttgattctc agaacgaaaa tacggaagaa
     1741 accatccgcc gactgcaccg gcaggaagaa aagtcgggaa tgcaggtaga aacaacgctg
     1801 cagcgtctgg agcattttta tgcggtagag cgctcgtatc tcagtctttt cctcgttttg
     1861 ggtgtattag gcgtgacact gggcgctttg gggataggcg tgatcacagc ccggagccgt
     1921 attgaaaggc gtgctgaatg ggcaatgctt caggtactgg ggtacgaaaa gaggcatttg
     1981 ctccgcttgc tggttgttga aaatgctgct attctgctgg ttgccgcgat tctgggcggg
     2041 ggtgcatctc tcacggctct tttgccgtct gtgctgcttt cctccaccac ccttcctctc
     2101 ctgcttcagt ttataggttt tttatccatg cttgctggcg gtgcgctcag cgttgctctt
     2161 gcgctgctag tgacccgatc ccaatcgctc ctgttagatc ttcgccgcga ataaaaattg
     2221 atatccgtgt aatcgaaagg ccgcaaaaaa ggtctaagta gtgcgaataa taaagattct
     2281 tcggagtgtt cgcattttaa gttggctcta tttgccaaag catggtaaaa tattgccaga
     2341 caagtatgcc tgttgatgca ggagtacttt tgaagatgaa agtggttatt tatccgggat
     2401 ctttttctgc aacattttgt acacctgagt tgtagtatac atcagtaaga aggcgagtgc
     2461 tatttaactg ggttaactaa ttgtgaatga gtactttatg aaaaaataca ttttcttcat
     2521 gctctgtttc tgtgctgtac ctcttctttt tggaacaggt tgcactgtac cggcggaagg
     2581 gactgttgat attacgctgg ctgctgcaga gccccccatc actgccacac ctgttcaggc
     2641 cgtgctcccg catcggggaa ctgtttcctc ctggtttgag actacgtctc gtgttgaggc
     2701 ggaggttcgg gtggatctgc tttccaaggg tacaggccag tgtgagaagg tgcatgtgaa
     2761 tgtgggggat cgtgttcagc aggggcaggt tgttgctgaa ttggaacgcc gagagctgga
     2821 agcgcaggca cggcagaccc gtgtgggcgt tcagcaggca aaaaccgctt acgagatagc
     2881 cgaacgcagt ttcaatgaag ggatcggcgc cagtgccgaa agagacaatg cccgtttcag
     2941 ttatgaacag gcaaaagcgg cggcggagct gatagatctg caaatctcca atcaaacgct
     3001 gcgtgcgcct atcagtggta tcgtgactcg caaaatggtg caggaaggca tggtggttgc
     3061 ggccggaatg cctgtcgtct caattgttga ccctgattct tttgttcttc ccatcgaggt
     3121 gccggaaaaa gagttgggtc gtctgaaaga agggcaggaa gctcaggtca gcattgatgc
     3181 ctttcctgat cgctcttttg ccgcccacgt cgttcgcatt gatcccagta tcaatcccct
     3241 gagcggtacc gttcgcatgc tcctcagctt tctagcggag gacaagcccc ttcttcgcga
     3301 agcggccttt gcccgtgtga aacttgcgat ggaaacaaaa ggcggcgtac tgctcgttcc
     3361 ccgggatgcc gtgatggaag aggaagggcg gaaatacgtt ttcgctctcg aaaaagagga
     3421 tgaaagtcag tataagcccg aacagctaga agaattgcga ggcaacgccc tttacgttgc
     3481 aaaacgcatt gacatcactg tgggtcttga gcagaatgaa aatattgagg tgctgaccgg
     3541 tctgaatccc gataacatgg tggcgatcat ggggcatcac tccttaaaag acggtgcttt
     3601 tgtaattctg actgatatgc agaaagaact ggccgcccgt tccgcactca cactggaaga
     3661 agcgctgctc gctgcgaaga aagcgaaaaa tcttatggtg ggatccgggc aggacggtcg
     3721 cagcagcgtt tcctctctct gagcatgctt catttatccc ggaaagagcc tcaggcactt
     3781 tcatctccca acggagattt cccgcctaaa ggcagtgggg cagggcgcac agggatggtg
     3841 tgtctgccga agtaaaaaaa tatataataa tggctgttgc agtgtacgcg gttttttagt
     3901 cgaacagaat catagaaaca cgttttcatg gcagatacat cttcaggttc cacaccccac
     3961 tatcgtttcg ccattcgtcg ccctgtttca acagccatgc tgtttctgac acttattgtc
     4021 ttcgggtggc gttcctacca gcaacttccg gtcaatctga tgccggacat ttcctatccc
     4081 agcctgacag ttcgtaccga atatgaaggt gcggcacctg aagatgtcga aatgcttgta
     4141 acccggccgc tggaggaact gcttagtatc gtgagcagta tggtggaaat cagcagcgtt
     4201 tcttctcccg gactttccga gattaccctg gaattcgact ggtcggcgga tattgcttct
     4261 gcgcagcagg
//
LOCUS       DAOPWB010000205         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_20865_length_4265_cov_5.367696, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000205 DAOPWB010000000
VERSION     DAOPWB010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20865_length_4265_cov_5.367696"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PKN92_12735"
     CDS             <1..610
                     /locus_tag="PKN92_12735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNZ49702.1"
                     /translation="IRNPLASLQSAVEELQRNLASPDMAQRLAAIALRESRHLNGIVT
                     SFLDFARNPEINREAMELGSFLDSLGTAYSEMHPDIEFSVIRGDTQCFIAADPVQMRQ
                     LCDNILMNSVEAMDGRGMIRVELTEKDRFAEIAFHDTGPGISPASIPRLFDPFYTEKK
                     HGVGMGLAVCLRIVTAHDGSIQAVPHADGGATIIVRLPKRSD"
     gene            637..2031
                     /locus_tag="PKN92_12740"
     CDS             637..2031
                     /locus_tag="PKN92_12740"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ49703.1"
                     /translation="MTKKHKVLVVDDETSICEVLEIVLSNAGYTVRTALSVEAAIDLL
                     ASENVDVVLTDLYMDTDRNAGLRLLEHLKSYLPKTPAIMMTAHGSIDTAIEAMRLGAV
                     DYVQKPFKSNEEMLLRIERALEKRWLIRENEAFRTEQAKRGSLDTMVGDSPVFVQVRE
                     LISRVAALPSTVAIQGESGVGKELVARALHSLSPRASKPFVAINCGGIPEMLLESELF
                     GYKKGAFTGAVQDKEGLFVIASGGTIFLDEIGEMPLRLQVKLLRVLDDNTVTPVGGIT
                     STTVDVRVLSATNRDLGDMVAKGEFRNDLYYRLNVIPIRVPSLRERKEDIPLLVRHLV
                     KKHSESMHLPAKKVSREAEEALLRYSWPGNIRELSNVLERALGLSEEDMLNLMDLPEY
                     IRKETLSQDAPSFEIPAEGFLLEDEIAKIEKEFLCKALEMSRNSPQKTAQLLGLNIRA
                     LRYRLDKYGLSTEQ"
     gene            2265..2831
                     /locus_tag="PKN92_12745"
     CDS             2265..2831
                     /locus_tag="PKN92_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49704.1"
                     /translation="MMRSIVINSCLILAVVLTGLGCARAARDTSGFALREETTVASGY
                     ENTWQTVKHVLREQGYEVYTMDKRGYFVAYTPLKRILWMQPKRTKFTVELARLSDNET
                     VIAVESIRQIYGVTLLTHPNWHDRKQNDPAPGAALLEGIRTKLAEGGAPAESFADEVD
                     AAAAKEEVLPVEEPAVIEAEDLTDPATP"
     gene            complement(2866..2991)
                     /locus_tag="PKN92_12750"
     CDS             complement(2866..2991)
                     /locus_tag="PKN92_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49705.1"
                     /translation="MTPLSWLYMGFIWLAIVALNAYCFYRIFAKKDSAEKQRTQQ"
     gene            complement(2988..>4265)
                     /locus_tag="PKN92_12755"
     CDS             complement(2988..>4265)
                     /locus_tag="PKN92_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HNZ49706.1"
                     /translation="GILLPAMVGVYYIYIESWSLGYAWQTATGAYWGQNSYESMGKVF
                     TSYLGISDSFFSFNPQSYFFFLIAFAVNIVVFSRGISRGIEVVARYCVPLLMLLGIVL
                     AVRVLTLPPQDGRTISDGLAQIWHVTDWRVLLKSDVWIAAAGQIFFTLSVGLGMIHTY
                     ASYLNKSDDVTLNGLSASAMNEFAEVILGGTIAIPAAVLFFGAAQTREIAEGGTFSLG
                     FFALPVIFQQMPGGQFFGTLWFFLLFLAGLTSSMAMFTPLLLFLEDELRANRKRAVNI
                     MGIVVFILMQPAIIFMHHGVVDELDYWMGEFCLVLFATIETVLFSWVFGAARGWKELH
                     YGAELQVPRIFFYIIKYVTPTFMLALLGWWVYDSLFDRVFMTGVDPASRPYLWLARLM
                     MMGMAGFLIWGVRRAWKTHPKFFEEADTEGERV"
BASE COUNT         1096 a         1089 c         1168 g          912 t
ORIGIN      
        1 aatccgcaat cccctcgcat cactgcaaag tgccgtggaa gaactgcagc ggaatctggc
       61 atcgccggat atggcacagc ggctcgccgc cattgcactg cgcgaatcga gacacctcaa
      121 cggcattgtt accagttttc tggactttgc cagaaatccc gagataaacc gggaagctat
      181 ggaactgggt tcttttcttg acagtctcgg cacggcatac agcgaaatgc atcccgacat
      241 tgaattttct gttatccggg gtgatacgca gtgttttatt gcggcagacc ctgttcaaat
      301 gcgacagctc tgtgataaca ttctgatgaa cagcgtggaa gctatggacg gtcggggtat
      361 gatcagagtg gaattgacag agaaagaccg ttttgccgaa attgcttttc acgatacggg
      421 ccccggtatt tcacctgcca gtattccacg tctctttgac cctttttata ccgagaaaaa
      481 acatggtgtc ggaatggggc ttgctgtatg cctgcgtatt gtcactgctc atgacggaag
      541 tattcaggct gtgccccatg cggacggcgg ggcaacaatc attgtccgcc ttccaaaacg
      601 ctctgactga aaagataagg atcatcagca aagattatga ctaaaaagca taaagttctg
      661 gttgtggacg atgagaccag tatctgcgag gtgctggaga ttgtcctgag caatgcaggc
      721 tatacagtca ggacggctct ttctgtggag gcggcaatcg acctgcttgc ttctgaaaac
      781 gtggatgttg tcctgacgga tttgtatatg gacacagacc gcaatgccgg actgcgcctg
      841 ctggaacatc ttaaaagcta cctgcctaag accccggcta tcatgatgac ggcgcacggc
      901 agtattgata cagccattga ggccatgcga ctgggtgctg tggactatgt gcagaagcct
      961 tttaaaagca atgaagaaat gctgctgcgc attgagcggg cgctggaaaa gcggtggctc
     1021 attcgggaga acgaggcgtt ccgtacagaa caggcaaaac ggggcagcct ggataccatg
     1081 gtgggtgaca gtccggtttt tgtgcaggtg cgcgagttga tcagccgcgt ggcggcactg
     1141 cccagtacgg tggcgattca gggagaaagc ggtgtcggta aagaactcgt tgccagagcg
     1201 ctccactcgc tgagtccccg cgccagcaag ccttttgttg ccatcaattg cggtggtatt
     1261 ccggaaatgc ttttggaaag cgaactcttc ggctacaaaa aaggcgcttt taccggtgct
     1321 gtacaggaca aagaagggct ttttgtcatt gccagcggtg gcaccatttt tctggacgaa
     1381 atcggcgaga tgccgctccg gctgcaggta aagctgctgc gggtgctgga cgacaatacg
     1441 gtgactcctg taggaggcat cacatccacc acagtggatg tccgtgtgct gtccgccacc
     1501 aaccgcgatc tgggtgacat ggtggcgaag ggagaattcc gaaacgatct gtattacagg
     1561 ctgaacgtga ttcctatccg ggtgccgtct cttcgggaac gaaaagaaga tataccgctg
     1621 ctggttcgcc atctggttaa aaaacatagt gaaagtatgc atctgcccgc caaaaaagtg
     1681 agcagggaag cagaagaagc actgctgcgc tatagttggc cgggcaatat ccgggagctg
     1741 agcaatgtgc tcgagcgggc gctcggtctg agcgaagagg atatgctgaa cctgatggat
     1801 cttcccgaat atattcgcaa ggagacattg tctcaggacg cgccttcttt cgaaataccg
     1861 gcggagggat ttcttcttga agatgaaata gcgaagatag aaaaagaatt tctgtgtaag
     1921 gcgcttgaaa tgagccgcaa ttccccgcag aaaacggcac agctgctggg cctcaatatc
     1981 cgtgcactgc gctaccgtct tgataaatac gggctgtcca cggagcagta gacaaattgt
     2041 gccagaagaa aagagctgtt ccccgccccg gtgtcggcta gcttggtgaa gcgaccgggt
     2101 aaagggctgc acccggaatt tttcacctaa aatcagaaaa agaatgcttt tctttacgta
     2161 cgccccgggg cagggaagca ctgagtggct tttcatgttt ccttttttga atgctcaaat
     2221 agaaaacggg tacaatgacc ccacttattc agggagaatc caccatgatg cgttccatag
     2281 tgatcaatag ttgtctgatt cttgcagtgg tgttgacagg gctgggctgt gcccgggccg
     2341 cccgggacac ctccggtttt gcccttcgcg aggaaaccac tgtcgcaagc ggctatgaaa
     2401 acacctggca gacggtgaaa catgtactgc gcgagcaggg ctacgaagtg tacaccatgg
     2461 ataaacgggg ctattttgtt gcctacacac cgctcaagcg cattttgtgg atgcagccca
     2521 agcgtacaaa atttacggtg gaactggcgc gcctgtcaga caatgagaca gtaatcgccg
     2581 tggagagtat ccggcaaatt tacggcgtga cactgcttac ccaccccaac tggcatgatc
     2641 gcaagcagaa cgatccggcg ccgggagcgg cactgctgga aggcatccgc accaaactgg
     2701 cggagggcgg tgcgcctgcg gagtcctttg ccgatgaagt tgacgccgca gccgcaaaag
     2761 aagaggttct tcccgtggaa gaacctgctg tgatcgaagc agaagatctg actgacccgg
     2821 ccactccgta agaaagcagc ggaagcgggt ttcattgtgc ctgtttcact gctgcgtgcg
     2881 ctgcttttca gcactgtctt tctttgcaaa aatccgatag aagcagtagg cgttcagcgc
     2941 cacaatagcc agccatatga atcccatata aagccatgac aggggggtca taccctttct
     3001 ccttctgtgt ctgcttcttc gaagaacttg ggatgagttt tccaggcacg ccgtacgccc
     3061 cagatcaaaa atcccgccat gcccatcatc atcagccgtg caagccacag atagggcctg
     3121 gatgccggat ccacaccggt cataaagaca cggtcgaaga gactgtcata gacccaccac
     3181 ccgagcaatg ccagcatgaa ggtcggtgtg acatatttaa tgatataaaa gaagatgcgc
     3241 ggcacctgga gctccgcacc gtagtgcagt tccttccagc cccgtgccgc cccgaaaacc
     3301 cagctgaaaa gcactgtttc aatagtggcg aaaagtacca gacagaattc gcccatccag
     3361 taatccagct cgtccacaac gccgtgatgc ataaaaataa ttgcgggctg catgaggata
     3421 aaaacaacaa tgcccatgat attcactgcc cgtttccggt tggcgcgcag ttcgtcctcc
     3481 agaaaaagca gcaggggcgt gaacattgcc atggatgagg tgagccctgc cagaaaaagc
     3541 agaaagaacc agagtgttcc gaaaaactgt ccgccgggca tttgctgaaa aataacaggc
     3601 agcgcaaaaa agccaaggga gaaggtgccg ccttcggcga tttcccgggt ttgtgcggct
     3661 ccaaagaaaa gcacggcggc agggatggct atggtaccac ccagaattac ctcggcgaac
     3721 tcgttcatgg cactggccga cagaccgttc agagtgacat cgtcactttt attcaggtag
     3781 ctggcatagg tgtggatcat gcccagacct acactcagcg tgaagaaaat ctgacctgcc
     3841 gcggcaatcc atacatccga tttcagcaat acccgccagt cggtgacatg ccagatctgg
     3901 gcaagcccgt cgcttatggt acgtccgtcc tgcggtggca gcgtcagcac acgcacggca
     3961 agaacaatgc ccagcagcat cagaagaggc acacaatacc gtgccaccac ttcgataccc
     4021 cgcgagatgc cccggctgaa tacaacaata ttgacagcaa aggctataag aaaaaagaaa
     4081 taggactggg ggttgaaact gaagaagctg tcgctgatgc ccagatagga agtgaacact
     4141 tttcccatgg attcgtagga attctgaccc cagtatgccc ctgttgccgt ctgccacgca
     4201 taccccagag accaggattc gatatagatg tagtacacgc ctaccatggc aggcagcagg
     4261 atgcc
//
LOCUS       DAOPWB010000206         3870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_23800_length_3870_cov_5.584010, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000206 DAOPWB010000000
VERSION     DAOPWB010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23800_length_3870_cov_5.584010"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..971)
                     /locus_tag="PKN92_12760"
     CDS             complement(63..971)
                     /locus_tag="PKN92_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ49707.1"
                     /translation="MATVLCIDQDEQYGSFVGLHLKKGGHSYIYTRFGAQALPLAEKH
                     APDLLISEVMLPDVCGFEIARRIRAHEDLHMLPIILTSTMCEEDEVRHALAQGIDGFL
                     PKPFNPAALLTLVKHHLTEVKNHPLKDTLTGLSGQKKIRAELQRAIALKHKFSALYIE
                     ITGLAEFARKESDSTRDKLLVYFTKVLDRCREKTAASLFHAGHFGNGHFFCIVDEDKA
                     TALGEHLQHYWKSRIEKDLAPLSATSADLPGGAEKNGLDLMIYITGNATSSRRSAQEY
                     FETLAQLRKKDQPQKGGIIIDNRRAR"
     gene            1322..3775
                     /locus_tag="PKN92_12765"
     CDS             1322..3775
                     /locus_tag="PKN92_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49708.1"
                     /translation="MTFRSMRPKHSLAFLSAVLLLLSLLFAPNALAVKRILLVGDSWA
                     QWPWKMNSFQTVLNSNFGAGVYEVEGTYTALGGTTANSWATNAVPPEDTFPSPPGYSN
                     MPSLDRITWSLNKWPTIDIIHLSISGNDMWAWRADWTPAQTQALYDTIRNDVHAVVNW
                     IRTNHPHVKILLCGYDYLNITETCTYGMQEFSWGSAILANTLGFSVGDFDQNRANNHQ
                     INLFFAGLGPRLVSLAQGINRLEYVQNWGNIQWRVGYKNSAGQIWGAHTVPFPGMAPD
                     YNPMPGGDVSWGTPSFYMNKTDGNQRDAIHLSNDGYKLLFDNCLMQYYAGWLTDTTGP
                     AVASITRNQPSPTALNQVSFTVLFNEAVRGVTVADFVLAGTATSGAVITGIAGAEDTN
                     ARVVTVDISGAPNGTLRLDLIDEGRIFDKYLNELGGDGVGNGNFNGGESYLIDRGDPV
                     PVITADIPEPSLLDTVPFTVKFNKAVTGFVASDIALQVKSGAGTAHVVNFAGSGDQYS
                     FGVMVTRSEPITVGISVPAGVCQDASNRPNLAGQYTDGADNAYAFNYVSGPVAPGDLY
                     VSDGSLGELVLTSGMALSINTGSTPPGFQINGGAPVFGQVFSATNGDVAKFNFSRVVV
                     PAGVTVSVTGNRPLVLAASGDMYWDSAIYVSGTVPGRAGGGTGGSGGAGGAGVSGGAG
                     GSSANGGAVAAGGAGSPSPSTDYGNGLGVGATGTAGKPGGGGANGAVGAAGKVGAAGS
                     AGTTGFGSQGSGGSAGTPGSAGTPLSQNNGGSAGPGGAPGGGGGPWRLGNEGQPADAG
                     GGCCCNRYRRQCRYRAERR"
BASE COUNT          849 a          950 c         1109 g          962 t
ORIGIN      
        1 cggcgtaggc tgcatcagga tgtctgatcg ggggcagggg ctgctctctg cacgcacgca
       61 gatcagcggg cacggcggtt atcgataatg atgccgcctt tctggggttg gtcttttttg
      121 cgcagctgcg ccagcgtttc gaaatattcc tgcgccgaac gcctggaaga agtcgcattg
      181 cctgtgatgt aaatcatgag atcgagaccg tttttctctg ccccccccgg aagatcggca
      241 gaagtggcag agagcggtgc cagatctttc tcaatgcgcg atttccagta gtgctgcaga
      301 tgctctccca gtgcggtcgc tttatcctcg tcaacgatgc agaaaaaatg accatttccg
      361 aaatgacctg catgaaagag cgaggcagct gttttttctc tgcagcgatc aagaaccttt
      421 gtaaagtaaa caagcagttt gtcccgggtc gagtcgcttt cttttcgcgc aaattctgcc
      481 aggcccgtaa tttcaatgta cagcgcggaa aatttatgtt ttaaagcaat ggcccgctgc
      541 agttctgcac ggatcttttt ctgaccgctt aagccggtga gtgtgtcttt cagcggatga
      601 ttctttacct ctgtgagatg atgcttcaca agagttaaaa gcgctgctgg attgaaaggt
      661 ttcggaagga aaccgtctat accctgcgcc agcgcatgac gcacctcgtc ttcttcgcac
      721 atggtggacg tgagaatgat ggggagcatg tgaaggtctt cgtgagcgcg tatcctccgt
      781 gcaatttcaa agccgcagac atcgggcagc atcacttctg aaatcagcag atcaggcgca
      841 tgtttttctg caagcggcag tgcctgggca ccgaagcggg tgtagatata ggagtgcccg
      901 ccttttttca aatgaagccc tacgaaactg ccgtattgtt catcctgatc tatgcaaagg
      961 acagtagcca ctgtgagaaa ctccctgcag aaaaaaatgg tttacccgcc tgtcgaagcc
     1021 cttttaaaaa tttgatgtgc tctgtgtata ttccaattct accataaatg cagccaaatt
     1081 atgtagtaac cggaaaactt atttattaat aataataata aatagtagca catttgcctt
     1141 gtaaaaacaa gaggttttta tcagtttctg cttgacacgg gaaaaagtta tgttatacta
     1201 taaaaaaatg ttaaatctgt tgaagatgga caaagaagag cgttgtgcgg tcttcggcga
     1261 gacgatacgg aatgactatt cgggatatgt cctatttata tcatttttaa ggagctttaa
     1321 gatgacgttc agatcaatgc gccccaaaca ttctcttgct tttttatccg cagttctgct
     1381 tcttttatcc ctgctttttg ccccgaatgc gttggctgtc aagcgtattc ttcttgtggg
     1441 tgacagctgg gcgcagtggc cctggaaaat gaattccttt caaacggtgc tgaatagcaa
     1501 ttttggtgcc ggtgtttacg aggtggaagg aacgtatacc gctctgggcg gcacaacagc
     1561 caacagctgg gcaaccaacg cggtgcctcc ggaagacaca tttccctccc cgccggggta
     1621 cagcaatatg ccctccctcg accgtatcac ctggtcttta aacaagtggc ctactatcga
     1681 cattatccat ttgtccattt caggcaatga catgtgggcg tggcgtgcgg actggacgcc
     1741 cgcgcaaaca caggcgctgt acgataccat tcgcaatgat gtgcatgccg tggtgaattg
     1801 gattcgcacc aatcatcccc atgtaaaaat tcttttgtgc ggttatgact atctgaacat
     1861 tacggaaacc tgcacctacg gcatgcagga attcagctgg ggctccgcaa tactggcgaa
     1921 tacgctggga ttttcagtag gggacttcga ccagaacagg gcgaacaacc atcagatcaa
     1981 tctttttttc gccgggcttg ggccccgtct ggtatctctg gcgcaaggta ttaatcggct
     2041 ggagtatgtt cagaactggg gaaacatcca atggcgcgtc ggctacaaga attctgcagg
     2101 tcagatctgg ggcgcacata cggttccctt cccgggcatg gcaccggatt acaatcccat
     2161 gcccggtggc gatgtaagct ggggcactcc ttccttttac atgaacaaaa cagacggcaa
     2221 tcagcgggat gcgattcatc tcagcaacga tggatacaaa ctgctgtttg acaattgcct
     2281 tatgcagtac tacgccgggt ggcttaccga tacaacaggt cctgcagttg cctccattac
     2341 acgcaaccag ccgtctccga cagcattgaa ccaggtgtcc ttcacggttc ttttcaatga
     2401 ggcggtgcgc ggtgtcacag tggcggattt tgtccttgcg ggaactgcaa cgtctggtgc
     2461 ggtgattaca ggaatcgcag gggcagaaga taccaatgcc cgggttgtca ccgtcgatat
     2521 ttcgggagcg cctaacggaa cgcttcggct tgatttgatt gacgaaggac gcatttttga
     2581 caaatactta aatgagctgg gcggggacgg tgtcggcaat ggaaatttca atggaggcga
     2641 aagctatctc attgaccggg gtgatcctgt accggtgatt acggcagata ttcctgaacc
     2701 ctctcttctg gatacagtgc ctttcacggt gaaattcaac aaggcggtta ccggattcgt
     2761 agcttctgac atcgctctgc aggttaagtc aggcgcaggg acagcacatg ttgtcaattt
     2821 tgcgggcagc ggtgatcagt attcctttgg tgtgatggta acccgctctg agccgattac
     2881 ggtcggaatc tccgttcctg ccggtgtgtg ccaggatgcg tctaacaggc cgaatctggc
     2941 agggcaatat acggatggtg ctgacaatgc ctatgccttt aattatgttt caggacctgt
     3001 tgcgccgggc gatctctatg tcagcgacgg ctctctggga gagctggtgc ttacttccgg
     3061 catggcgctg agcattaaca cgggctcgac gccacccgga tttcagatca atggcggcgc
     3121 tcccgttttc gggcaggtct tttccgcaac caatggtgat gttgccaaat ttaatttctc
     3181 ccgggtcgtt gtgcccgccg gggttaccgt ttcagtaacg ggcaaccgcc cgcttgttct
     3241 ggcggcttct ggcgatatgt actgggattc agccatttat gtttccggta ctgtgcccgg
     3301 tcgtgccggt ggcgggacag gtggttccgg cggcgctggc ggtgcaggtg tatccggcgg
     3361 cgctggcggc agttcggcaa acggcggtgc ggttgctgca ggtggtgcag gcagcccaag
     3421 tcccagcaca gactacggta atggactagg tgttggagcg accggtacag cgggcaagcc
     3481 gggaggagga ggcgcaaacg gtgcggtcgg agcagcaggc aaggtcggtg ctgccggcag
     3541 cgccgggacg actggcttcg gcagtcaggg ttccggcggt tctgcaggaa cgcccggatc
     3601 ggcaggcact cctttatccc agaataatgg tggttctgcc ggtcccggcg gtgctcccgg
     3661 cggtggcggc ggtccctggc gtttgggtaa tgaaggacag cctgcagatg caggtggggg
     3721 gtgttgctgc aaccggtacc ggcggcaatg ccggtacagg gcagaacgga ggtaatggcg
     3781 ggggtggtaa tgccggcggt aatgccgcat taacgatcgc tgcagacgcg ctgcttctgg
     3841 ctgcaggcaa tggcggcggt ggcggtggcg
//
LOCUS       DAOPWB010000207         3850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_23954_length_3850_cov_5.263768, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000207 DAOPWB010000000
VERSION     DAOPWB010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23954_length_3850_cov_5.263768"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="PKN92_12770"
     CDS             <1..1786
                     /locus_tag="PKN92_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ49709.1"
                     /translation="PVAFLGGSTGVMYRQFGITIAMAVAVSGVVALTLTPALCAALLK
                     PNHNKLFIFRWFDKSFDFVSSGFMVSVKWVIRLGVISVLLFGALIWGAYTLMSRVPTA
                     FVPQEDQGYFFAVVQLPPGASLNRTIEVVKQVEEFLLAQPEVEGLTAMCGQDNLSGAN
                     TTNVASMFVVLRHWDERPNPENSAQSVVGRVFGRFGAMKEATVLAFIPPPVSGLGLRA
                     GIEAQLEARGSSDINELAEVMHGFVARLNADPLFEAVSGVLTVEQPKIRVNLNQTRAK
                     VLGIPIGEIYNTLQAYLGSYYINDFNVFGRVYRVQLQADSAFRDAPDDIRKIHVRNRS
                     GDMIELAGVLDVAMDAGPNVVSRFNSFSSVQITGAPAPGVSTGQLIRRVQELAAEHLP
                     PGYAVEWSSGSYQEIKAGNQSVYVIIFGMIMVFLVLAAQYEKWSLPVTVLLTVPFAAF
                     GAALWVWYKNGEMDIYFQIGLLTVVGLTAKMAILVIEFCAVERAEGRGIVEAALEAAR
                     LRLRPVMMTAVTTILGALPLVLSTGAGAAGRRSIGGSVMGGMISACFLAIFFIPLFFV
                     IVQWLSELFKKPKPVTAGDAPADKGATS"
     gene            1783..3360
                     /locus_tag="PKN92_12775"
     CDS             1783..3360
                     /locus_tag="PKN92_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNZ49710.1"
                     /translation="MKSLRYTVSFLVAVFLAGCMTGPDYQRPDLATPGNWSQPMQGGE
                     KADTPEAVHWWKEFSDSRLDALVSRALAANLDIRLAESRIREARGNLVRTGAALWPQL
                     NVSGSYQRRQIAEINTSRPPSARANAVFSKNGLTGLSISGTSPAGPGVSITPDLRGGG
                     NTTVGVSAGTESLAKKPDRQMDLYQTGFDASWELDIFGATRRAKEAARAEIEAAEENR
                     RAVYISVAAEMARSYFDLRALQHRLAIARSNTATVENSLQLVQARFDAGLTSELDVRR
                     AEAQLANTRALIPALEYGIEQAIHRMSILCAEIPGALREELITTEELPDAPPEVLVGI
                     PSELLRRRPDIRRDERLLAAATARIGVATADLFPKFMLTGSLMGSASDFSGLSLGANR
                     IWSIGPAIRWPVFDAGRIRANIDIQNARQEQALLSYEKTVIQSFAEVENALVLYAQEQ
                     NRFEELGKSVEAGEKALAIANDLYAQGLVSYLEVLDAERTLFTARDQRLQSQVSVLTS
                     LVSLYKALGGGWEESTV"
     assembly_gap    3579..3678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          743 a         1061 c         1071 g          875 t
ORIGIN      
        1 gccggtcgcc tttctcggtg gcagtacggg ggtcatgtac cgccagttcg gtattaccat
       61 tgccatggca gtcgccgttt ccggtgtagt tgcccttaca ctgactccgg cactctgtgc
      121 ggcgctgctg aaaccgaacc acaacaaact ctttatcttt cgctggttcg ataaatcttt
      181 tgattttgtg tcatccggtt ttatggtctc cgtgaaatgg gtgatccgtc ttggggtgat
      241 ttccgtgctg cttttcggcg cactgatctg gggtgcctat acactgatga gccgtgtgcc
      301 tacagctttc gtgcctcagg aagatcaggg ctatttcttt gctgtagtgc agctgccacc
      361 gggcgcctca ttgaaccgca ccattgaggt ggtcaagcag gtggaagaat ttctgctggc
      421 gcagcctgaa gtggaaggac tgacagccat gtgcggccag gacaatctct ccggagccaa
      481 cacaaccaat gtggcgagca tgtttgttgt cctgcgccac tgggacgagc ggcccaatcc
      541 tgaaaacagt gcccagtcgg tggtggggcg tgttttcggt cgctttggtg ccatgaaaga
      601 agcaacagtt ctcgctttta tcccgccgcc tgtgtcgggg ctcgggcttc gtgccggtat
      661 cgaagcgcag ctggaggcac ggggcagcag tgatattaac gagcttgccg aggttatgca
      721 tggctttgtc gcacggctga acgcggatcc tctttttgag gctgtatccg gagttctcac
      781 tgtcgagcag cccaaaatcc gggtgaatct caaccagacc cgtgccaaag tgctgggcat
      841 tcctatcggc gaaatttaca ataccctgca ggcttatctg ggctcctatt acattaacga
      901 tttcaatgtt ttcggccgtg tttaccgcgt gcagctccag gcggactccg cattcagaga
      961 tgcgcccgac gatatcagaa aaattcatgt gcgcaaccgc tccggcgata tgatagagct
     1021 ggcaggggtg ctcgacgttg ccatggatgc gggtcccaac gtggtgagcc gattcaacag
     1081 cttctcgtct gtccagatta ccggcgctcc ggcaccgggg gtcagtacgg gtcagttgat
     1141 ccggcgcgtg caggagttgg cggcggaaca tctgccgccc ggctatgcgg tggaatggag
     1201 ttccggttcc tatcaggaaa tcaaggcggg caatcagtcc gtctacgtaa ttattttcgg
     1261 tatgatcatg gtgtttcttg tccttgctgc ccagtacgaa aaatggtcgc tcccggtgac
     1321 cgtgctgctg actgtgcctt ttgcggcctt cggcgcagcc ctctgggtct ggtacaagaa
     1381 tggagagatg gatatttatt tccagatcgg cctgctgacg gtggtcgggc ttaccgctaa
     1441 aatggcgatt ctggtcattg agttctgcgc tgtcgaaaga gcggaaggca gaggcattgt
     1501 ggaggcggct ctggaagcgg cacggctgcg gctgcgtccc gtgatgatga ctgcggtgac
     1561 aaccattctc ggtgcattgc cgctggtgtt gagcaccggt gccggggcag cgggacgtcg
     1621 ttccatcggc ggcagtgtga tgggcggcat gatttccgcc tgctttctcg ccattttctt
     1681 tattcctctt ttcttcgtta ttgtgcaatg gctcagcgaa ttgtttaaaa agccgaagcc
     1741 tgtgactgcc ggagacgccc cggcagataa aggagcgact tcatgaaatc actccgctat
     1801 acagtctcct ttcttgttgc agtctttctg gcgggctgca tgaccggacc cgattatcag
     1861 cgccctgacc tcgccacgcc cgggaactgg agccagccca tgcagggcgg cgaaaaagcg
     1921 gatacgcctg aagcggttca ctggtggaaa gaattcagtg actccagact cgatgcgctg
     1981 gtgagccgtg ctcttgccgc caatctcgac ataaggcttg cagaaagccg aatccgggaa
     2041 gcgcggggta atctcgtgcg caccggtgcc gcgctctggc cgcagctcaa tgtctccggc
     2101 tcctatcagc gcaggcagat tgccgaaatc aatacgtccc gtccgccgtc ggcacgggcg
     2161 aatgccgtgt tttcgaaaaa cgggctgacc ggactgtcca tctccggaac ttcaccggcc
     2221 ggaccgggcg tttccatcac acccgatctg cgcggcggcg gaaacactac cgtgggtgtt
     2281 tctgcaggca ctgaaagcct cgccaaaaaa ccggatcggc agatggatct ctatcagaca
     2341 ggctttgatg cctcctggga actggatatc ttcggcgcca caaggcgtgc aaaggaggcc
     2401 gcgcgcgctg aaatcgaagc tgcagaagaa aaccgccgtg ccgtgtatat ttccgtagcg
     2461 gcggaaatgg cgcggagcta ttttgatctc cgcgctttgc agcaccggct tgccattgcc
     2521 cggagcaaca ccgccacagt ggagaattca ctgcagctgg tgcaggctcg ttttgatgcc
     2581 ggactgacca gtgaactgga tgtacgccgc gctgaagcgc agctagccaa tacccgggcg
     2641 ctgattcccg ccctggaata cggcatcgag caggctattc atcggatgag tattctctgc
     2701 gctgaaatac ccggagctct gcgtgaagaa ctgattacca cggaagagct gcccgatgcg
     2761 ccgcctgaag ttctggtggg tattccgtcc gaattgctcc ggcgccgccc tgatatacgc
     2821 agagacgagc gtctactggc ggcggcaacg gcgcgtatcg gtgtggcaac ggccgatctt
     2881 ttcccgaaat tcatgctgac cggatctctg atgggctccg cttcagattt ttcggggctg
     2941 agcctgggtg ccaaccgcat ctggtctatc ggcccggcaa tccgctggcc ggtctttgat
     3001 gccgggcgta tccgtgccaa tatcgacatt cagaatgccc gtcaggaaca ggcactgctc
     3061 tcctatgaaa aaacagtgat ccagtctttt gcggaagtgg agaatgcgct ggtgctgtat
     3121 gctcaggaac agaaccgttt tgaggaactg ggcaaaagcg ttgaagcagg cgaaaaagcg
     3181 ctcgctattg ccaatgatct ttatgcgcag gggctggtca gctatctgga agtgctcgac
     3241 gccgaacgca cgcttttcac tgcccgggat cagcggctcc agtcgcaggt ttctgtgctt
     3301 acctcgctgg tatccctgta taaagcactg ggcggcggct gggaggaaag cactgtctag
     3361 ctgcagatga actgttttgt tcctgtacca caatactctg actgaaagca ggctatccgc
     3421 attcctgggg gaagaattgc ctttcccctg tcacacctgc tccacactct ccgccgtcat
     3481 tccagctccc ccgccgtcaa caccgcactc tccgctgtcc ctgcactctc cgccgtcatt
     3541 ccagctcccc cgccgtcaac accgcactct ccgctgtcnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnncc tgcactctcc gccgtcattc ctgcgcaggc aggaatctta
     3721 atcaggatat ggacgtcttg atctgcacat cgccctgatc cacaccaaag gtttcaagac
     3781 atacaaacac aaaagaatcc ttactttcaa agactttccc gcgtagttga cggcaacagc
     3841 atgaccctta
//
LOCUS       DAOPWB010000208         3797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_24411_length_3797_cov_5.277392, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000208 DAOPWB010000000
VERSION     DAOPWB010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24411_length_3797_cov_5.277392"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PKN92_12780"
     CDS             <1..651
                     /locus_tag="PKN92_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49711.1"
                     /translation="NYKRDHMPVDESLPPLPERPYTVAFVTQTTVTKREMIDGQSIPV
                     EFDVLSYRQYEDSKEDRSESKVVLSDVKLNPDFKALHAFALTIPHGADVDYTYPRDPD
                     SNTVRSISSTWEGDKIVFDSDDQEMVAKLDKFVAGATIEKGFLPGKMRMKLAKLRYKI
                     AKTSFLTWYHMKNSMTLDWSTLLLSLMAIGGCFFVAGRIIYRKGAASRDKERPTAA"
     gene            complement(748..1311)
                     /locus_tag="PKN92_12785"
     CDS             complement(748..1311)
                     /locus_tag="PKN92_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNZ49712.1"
                     /translation="MQDTDCKKRIEIVQGDITQEKVDAIVNAANNTLLGGGGVDGAIH
                     RAGGPAILEECRALKGCNTGDAKITTGGNLKAKYVIHTVGPIYKDGARNEAELLASCY
                     RRSLEVAVENGVRTIAFPAVSCGVYGYPLEEAAQIALETTAAFLKSSVGIELVRFVLF
                     NDALFRIFQKVLEHVGDVQGSQQNDTI"
     gene            1804..2733
                     /locus_tag="PKN92_12790"
     CDS             1804..2733
                     /locus_tag="PKN92_12790"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008354.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNZ49713.1"
                     /translation="MQRYFQTAFFLFIAAALFCAPTFSEKIVIAHRGASGYVNEHTLA
                     AYAMAYGMGADYIEQDLGMTKDGVLICMHDRTLDRTSNVADMFPERKREDGKYYPEDF
                     TLEEIKTLRCVESVPKRFPREARLLQMVTFEEAIQLVQGLNRSTGRTVGIYPELKDAA
                     AYENAGLPYLKTFMETLARYGYEGADATVYVQCFSSGILKRLKEEYKCSVPMIQLMSS
                     DMVEEADMKEVATYAQGIGPDKKAIARDPSVVSRAHEAGLKVHPYTFRSDAFPSKFKD
                     AAEEMHLFLFEYDIDGGFTDHPDVMRRVIDAGN"
     gene            2764..>3797
                     /locus_tag="PKN92_12795"
     CDS             2764..>3797
                     /locus_tag="PKN92_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNZ49714.1"
                     /translation="MMLKDNTAVSYARAWALFLVSYAVFFGITLFMGKFIEADWDIRL
                     LLFLNPDHYVPILDQLVIFQTDWGAFYMGLLTIAWLVGYYASRSSAEAQTRTKYVYYV
                     LAVLFGLWYGAGMFVQKKGIFWWGKAEYTSVFLLLGLVLFGGLMMIGTLFTRLSDDDQ
                     RKITQACWLVLLCVFFVNPVGEDTIKALIKRPRPLHEANNAWNGAIRVLPDEVVKGSY
                     SYISGHTSSFWAQTVVFFWLIKSWRIRIPLLALGIFHGYTRIYTTAHFPYCVIMATFF
                     GFGVSSMVYYCFWNHRHLPLLAMLSVGASLLMVAKASQRPVALGVIVISLVWFAVWHL
                     INRGKAPAPAV"
BASE COUNT          866 a          915 c          989 g         1027 t
ORIGIN      
        1 aactacaaac gcgaccacat gcccgtcgat gaaagtctgc cgcccctgcc ggagcggccc
       61 tacacagtcg catttgtgac ccagacaacg gttaccaaac gggagatgat cgatggccag
      121 agcatacccg ttgaatttga tgtcctgagc taccgacaat acgaagacag caaagaagac
      181 agatcagaga gcaaagttgt cctctccgat gtaaagctga acccagactt taaagcgctt
      241 catgctttcg ccctcaccat tccacacgga gccgatgtcg attataccta tcccagagat
      301 cccgatagca atacggtgag atccatttct tcgacctggg aaggtgacaa gatcgtcttc
      361 gactccgacg atcaagagat ggttgcaaaa ctagacaagt ttgtggcagg agccaccatt
      421 gaaaaagggt ttctgcccgg aaaaatgcgc atgaagcttg ccaaattgcg ctataagatt
      481 gctaaaacaa gcttcctcac ctggtatcac atgaagaatt ccatgacgct ggactggagc
      541 acgctgttgc tgagcctgat ggcgattggc ggctgtttct ttgtcgccgg tcgaatcatc
      601 taccgtaaag gagctgcttc gagagataag gaaagaccca ccgctgcata agcgagctat
      661 agcctgaaaa aaaacagtga ccactgtgct cttaaacagg tacagtggtc actttgtctt
      721 ggggcagttt tagatcagaa gtgaaaatta gatcgtatcg ttttgctgtg atccttgcac
      781 atccccgacg tgctccagaa ctttttgaaa aatccggaag agggcgtcat tgaaaagtac
      841 gaaacgaacc agttcgatgc ccacggagct tttcagaaaa gcggcggttg tttccagtgc
      901 gatctgcgcc gcttcttcca gagggtagcc gtatacgccg cagctgacgg cagggaaggc
      961 gatggttctg acgccgtttt ctaccgctac ttccaggctg cgcctgtagc atgatgccag
     1021 cagttccgct tcgttgcggg cgccgtcttt gtagatgggc cctactgtgt gaatcacgta
     1081 tttggctttg agattgcccc cggtggtgat cttcgcatcc cctgtgttgc agcccttgag
     1141 agcgcggcat tcttccagaa tcgccggacc gccggcacgg tggatggcac cgtccacgcc
     1201 accgccgccc agcagcgtat tgtttgccgc atttacaatg gcatccacct tttcctgtgt
     1261 aatatcgccc tgtacaattt cgatacgttt tttgcagtct gtatcttgca tgggcagttt
     1321 cctcctgtac ggatgattct ctgtgtaata cacaattttg atgtgaaaag acaatttttt
     1381 gttaaaagag tgttaacgcc atagcagatt ttgtgtgaat cgacagcggg acagcatgag
     1441 cggtgcagaa cagagagatt ttttgaacat cagcgttcct ttatgattcc aggaggcctc
     1501 gtgtcgcttc aatgaccttg ccaaagaaac ctttcccaat gcacctttgt gccctctttg
     1561 ccctctgtgg ttgatcttct tcgtgaacct tcgtgccgct tcgttccttc gtggcttaaa
     1621 agatgtttgc ttgtgccata tagtgtggtg tccgctccgc ccgtgacgtt tactccttcg
     1681 tttttttgtt tttttttcag tacatctgtt ataacagacc catgcgaaaa acagcccggt
     1741 ctgcagcagg aggcagtgcg cctctgcttt tttgttttta tggatcgggc ggaaaggatt
     1801 ccgatgcagc gttattttca aacagcattt ttcctattta ttgccgccgc ccttttttgt
     1861 gccccgacat tttctgaaaa aattgtgatt gctcatcgcg gtgccagtgg ctatgtgaac
     1921 gaacacacct tggctgccta tgccatggcg tacgggatgg gagccgatta cattgagcag
     1981 gatctcggga tgaccaaaga tggcgtgttg atttgcatgc acgaccgcac gttggatcgc
     2041 accagcaatg tggcggacat gtttcctgag cggaagcggg aagacggcaa atattacccc
     2101 gaagatttta ctctggaaga gattaaaacg ctccgttgtg tggagagtgt tccgaaacgt
     2161 ttcccacggg aagcacgcct gcttcagatg gtgacttttg aggaggcaat tcagctggtg
     2221 caggggttga accggagcac agggcgcacc gtgggaattt atccggaact gaaagatgcg
     2281 gccgcatatg aaaacgccgg actgccttat ttaaaaacgt ttatggagac gctggcgcgt
     2341 tacggttatg agggtgcaga tgccactgtc tatgtgcaat gtttttcttc aggcatccta
     2401 aaacgactga aagaggagta taagtgttcc gtaccgatga ttcagctcat gtccagcgac
     2461 atggttgaag aggcggatat gaaagaagtg gcgacttatg cgcaggggat cggcccggat
     2521 aaaaaagcga ttgcccggga tccgtccgtg gtgagtcggg cacatgaagc cggactgaaa
     2581 gtacatccct acaccttccg ctccgatgct ttcccgtcca aattcaaaga tgcggctgaa
     2641 gagatgcatc tgtttctttt tgaatatgat atagacggtg gttttactga tcaccccgat
     2701 gtgatgcgtc gtgtcattga tgccggcaac tgaatcagta ccttcccata aggattttac
     2761 attatgatgc tgaaagacaa tacagcggtc agttacgccc gcgcctgggc gctttttctc
     2821 gtatcctatg ccgtcttttt cgggattacc cttttcatgg gcaaatttat agaagcggac
     2881 tgggacatca gactgcttct ttttctgaac ccggatcatt atgtgccgat actggatcag
     2941 ctggtcattt tccagactga ctggggtgcg ttctatatgg gactactgac catcgcctgg
     3001 cttgtcgggt attatgcgag ccggagcagc gcggaggcac agacaagaac aaaatatgtc
     3061 tattatgtgc ttgcggtact gttcggcctg tggtacggag ccggtatgtt tgttcagaaa
     3121 aaaggtattt tctggtgggg taaagctgag tataccagcg tatttcttct tttgggactg
     3181 gtgctgttcg gcgggctcat gatgatcgga acgctcttca cacggctgag cgacgatgat
     3241 cagcgcaaaa taacccaggc atgctggttg gtgctgctgt gcgtcttttt tgtcaatccc
     3301 gtcggagaag acacgattaa agcccttatc aagcgacccc gcccgctgca tgaggcaaac
     3361 aatgcgtgga atggtgcgat ccgtgtgctt cccgatgaag tggtgaaggg aagttattcc
     3421 tatatatcag gacacacttc cagcttctgg gcgcagacag tggtcttttt ctggctgatt
     3481 aagtcgtggc gaattcgtat tccgctgctg gcactgggta tatttcacgg atatacccgc
     3541 atctatacta ccgcccattt tccttattgc gtgatcatgg caaccttttt cggtttcggt
     3601 gtgagctcca tggtctatta ttgtttctgg aatcatcgcc atcttccgct gctggcgatg
     3661 ttgtccgttg gtgcctcgct tttgatggta gccaaagcgt cacagcgtcc cgttgccctg
     3721 ggtgttattg taatcagtct ggtatggttt gctgtatggc acttaataaa ccgcggtaaa
     3781 gccccggcac ctgcagt
//
LOCUS       DAOPWB010000209         3750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_24839_length_3750_cov_4.780785, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000209 DAOPWB010000000
VERSION     DAOPWB010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24839_length_3750_cov_4.780785"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="PKN92_12800"
     CDS             <1..1283
                     /locus_tag="PKN92_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNZ49715.1"
                     /translation="NDAVYATLITLCVDICQRNGKKKLIWLANKDKTLNYTPASDEMV
                     LTVHRWFANKSCPGDWLYNRLSDLAAKVTAKLGGSTTTTTGTQATEFANLTETQVIAK
                     VGPLFTANQKSSGILASVSLAQFILESGYGKSELAQNANNCFGMKKSLSGNTWSGSTW
                     DGSSVYTKKTQEQNSDGSYTTITADFRKYPNVEDSIADHSAYLRGAKNGSALRYAGIA
                     GMTEYKAVAQLIKDGGYATSLTYVDNLCSIIEKWNLTQYDAAATGSSSNGTGSTISVS
                     DLPSVPFTVKVLIDDLNYRSEGSMSGTVKGQTGKGTFTITEVNSAGWGRLKSGAGWIY
                     LLNPSYCTIGSTVANSTSTSSVPYTVKVTISDLNIRKGPGTNYAKTGKYTGKGVFTIV
                     EESAGSGSTKGWGLLKSYQKNRDGWVSLEYATKQ"
     gene            1321..1785
                     /locus_tag="PKN92_12805"
     CDS             1321..1785
                     /locus_tag="PKN92_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49716.1"
                     /translation="MGRRFLWVSLHTRHFNFIGGNDIVQRLTDTQKTAIRNLRASGYG
                     YGAIAQQLSLSRETVRSFCVRNNKADEPCAGITDSTGTDGPITDGPRADSLAPHDLSA
                     ENASEFRSGNTVFIIKTGYSETASETLEQKLKKLILADASKPSESYPFVQNY"
     gene            1850..3469
                     /locus_tag="PKN92_12810"
     CDS             1850..3469
                     /locus_tag="PKN92_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNZ49717.1"
                     /translation="MVRATSNTEKIAALYCRLSRDDGGDAESNSIGNQKTILSRYAAE
                     HGFPRTKFYVDDGWSGANFSRPGFEAMMSDVDAGLIGTIICKDMSRFGRDYLHVGLYT
                     EIKFPEAGIRFIAINDGVDSASGVSDDFTPFRNIINEWYCRDISKKIKASMQSRAKSG
                     EHLTGNPPYGYKKSESNPKLWVIDEEPAKIIREIFQLYLNGNNATQIAAEMAGRGYDA
                     PGDYLTKQHQYTKGKAVTFETPKAMWHPGTILNLIDRYEYCGHTVSYRRKSVSYKSHK
                     TVLNPEDDWIITKNTQDAIIDEETWQTVHKMRESGRRRKEHIWDKGPLNGFLYCPDCG
                     SKLYFSHPTRLKTRGTYMCGYYMHYKKCSTHYIRRDELEPAVLAQLRADCAFAKEHED
                     EFVKMVEKKTRRHGDNAVKKGEKEYAEARSRIEEIDQIINQLYEDKVSGGLSAERFSR
                     MLAKYEAEQETLRTKCEKLQEQIAAARTTSDNAKQFIRVVRKFTEMQELTPEIVSTLI
                     ERVEVGQAQMVDGVKKQEIKIIYNFIGNIQE"
BASE COUNT         1071 a          896 c          982 g          801 t
ORIGIN      
        1 tgaacgatgc agtttatgcc acactgatta ctctctgcgt agatatctgc cagagaaacg
       61 ggaagaagaa actgatctgg cttgccaata aggacaagac gctgaattat accccggcat
      121 cggacgagat ggtactcacg gttcatcggt ggttcgccaa taagtcgtgc cctggggact
      181 ggctgtacaa tcgtttgtct gatctggcgg caaaagtaac ggcaaagctg ggcggaagca
      241 ctacaaccac cacaggcaca caggcgacag agttcgctaa tctcaccgag acgcaggtaa
      301 tcgctaaagt aggaccgctc tttaccgcaa accagaagtc ctccggcatc ctggcatcgg
      361 tatccctggc acagtttatt ctggagagcg gttacggtaa gagtgagctt gcccagaacg
      421 ccaacaactg ctttggtatg aaaaaatctc tgtccgggaa cacctggtct ggctccacct
      481 gggatggcag ttccgtctac accaagaaaa cgcaggaaca gaacagtgat ggcagctaca
      541 ccaccatcac agccgatttc cgtaaatacc ctaatgtgga ggactctatt gctgaccatt
      601 ccgcctatct gcggggtgcg aagaatggct cggctctccg ctacgccggg atcgccggga
      661 tgacggagta taaggctgta gcgcaactca ttaaagacgg cggctatgcg acctcgctga
      721 cctatgtgga caacctttgc agcatcatcg aaaagtggaa tctgacgcag tatgatgcgg
      781 ccgcaaccgg gagcagtagc aacgggacgg gcagtaccat ctcagtatcc gatctcccgt
      841 ctgtcccttt cactgtgaag gtcttgattg atgatttgaa ttataggagc gaaggctcca
      901 tgagtggaac ggtcaaaggc cagaccggga aaggcacatt cacgatcaca gaagtgaatt
      961 ctgccggatg gggcaggctg aaatccggcg cagggtggat atatcttctg aatccttcct
     1021 actgcacgat tggcagtaca gttgccaaca gcacctcgac ttcctctgtt ccctacacgg
     1081 tgaaagtcac gatcagcgat ctgaatatcc gtaaaggacc tggcactaac tatgccaaaa
     1141 ccgggaaata tacggggaaa ggcgtgttta ccattgtaga agagtccgcc ggttccggct
     1201 ccaccaaagg ctggggactt ctgaaatcct accagaagaa ccgtgacggc tgggtgagtt
     1261 tggagtacgc tacgaagcag taaaactatc ctggacggcc cacagagcat tacctgttct
     1321 gtgggccgcc gttttctctg ggtgtcatta catactaggc atttcaattt tataggaggt
     1381 aatgatatcg tgcaaagact gacagacacc cagaaaacag cgatacggaa cctgcgggct
     1441 tccggttacg gatatggagc gattgcgcag cagctttccc tttcccgcga aacagtacgc
     1501 agtttttgtg taagaaataa taaggcagat gaaccttgcg ccggaataac cgactcgact
     1561 ggtacagacg gcccgataac agacggcccg agagcagaca gcctggcacc acacgacctg
     1621 agtgcagaaa acgcatccga attccgatcc ggcaataccg tctttatcat taagaccggc
     1681 tacagcgaaa cggcgtcaga aactctggag caaaagctga aaaaactcat cctggccgac
     1741 gcttcaaagc cgtcggaaag ttatccgttt gttcaaaatt attgacttgc tatttccagt
     1801 tgtcagagca atatatagta ctgaacaatc gaattgaggg agtgataaaa tggtaagagc
     1861 aaccagtaac acagaaaaga ttgcggcgct ttactgccga ctttccagag acgatggcgg
     1921 agatgccgaa tcaaacagca tagggaatca gaaaacgatc cttagccgct acgctgcgga
     1981 gcatggcttt cccagaacaa aattctatgt tgacgatggc tggagcggcg cgaatttcag
     2041 caggccggga tttgaagcca tgatgtcgga tgtggatgcc gggctgatcg ggacaattat
     2101 atgtaaagat atgtccagat tcggcagaga ttacctgcat gttggtttat acacggagat
     2161 caaattcccg gaagccggga tacgatttat cgccattaac gacggtgtgg acagcgccag
     2221 cggcgtttcc gatgacttta ctcccttccg caacatcatc aacgaatggt actgccgcga
     2281 tatttctaag aaaatcaagg cgagtatgca gtcccgcgca aagtccggag agcatctgac
     2341 gggcaatccc ccgtatggtt acaaaaaatc tgagtcaaat cccaagctat gggtgataga
     2401 cgaggagccg gcaaaaatta tccgggagat tttccagctt tacctcaacg gcaataacgc
     2461 cacccagatc gccgcagaaa tggccggtcg cggttatgat gcccccggcg attacctcac
     2521 gaaacagcac caatacacca aaggaaaggc tgtaacattt gaaaccccga aggcgatgtg
     2581 gcatcctggg acgatcctga atctgattga cagatacgaa tactgcggtc ataccgtcag
     2641 ctatagaaga aagtcggttt cctacaagtc ccataagacc gtactcaatc cggaagatga
     2701 ctggattatc acaaaaaata cgcaggacgc tatcattgat gaagaaacct ggcagaccgt
     2761 tcataagatg agggaaagcg gcagacgcag aaaagaacac atctgggaca aaggtccgct
     2821 gaatggattt ctctactgcc ctgactgcgg cagcaaactt tatttcagtc atccgacgcg
     2881 gctcaaaaca agaggtactt atatgtgcgg atattatatg cactataaga aatgctccac
     2941 acattacatc cgtcgggacg aactggaacc ggccgtgctg gcacagctgc gcgccgactg
     3001 cgcctttgcc aaagagcatg aggacgaatt cgtaaagatg gtggagaaaa agaccagacg
     3061 ccacggagac aatgccgtga agaagggcga aaaggaatac gcggaggcca gaagcaggat
     3121 tgaagaaatc gatcagatca tcaaccagct ttatgaggat aaggtcagcg gtggtctgag
     3181 cgcagagcgg ttttccagaa tgctggctaa atatgaagcg gagcaggaaa cactccgcac
     3241 aaaatgtgaa aaactgcagg agcagatcgc cgccgccaga acgacatcag ataacgcaaa
     3301 gcagttcatt cgagttgtca ggaaattcac ggagatgcag gaactgactc cggagatcgt
     3361 ttcgacgttg attgaacggg tcgaggtcgg acaggcgcag atggtcgatg gggtcaagaa
     3421 gcaggaaatc aaaatcatct acaacttcat aggaaatatc caggaataag ctctgcggga
     3481 gaaagagccg tggagcttac atttttatcc ccgtttatat cgattatatt gcttatattg
     3541 gttatattgc tcatatcact cattccccgc tgtcggtgtt ggcttaaggc tggtatcgat
     3601 agcggggctt ttttgcgttt tgggggtgtg caaaagtatt caccttgcaa gataactccc
     3661 atgagccgat tattccaagg gagcttttta tgcaagttca agaagagatg gttcgaagag
     3721 caaatcttcg tggccgcaaa ggcggtaaaa
//
LOCUS       DAOPWB010000210         3742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_24912_length_3742_cov_5.324654, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000210 DAOPWB010000000
VERSION     DAOPWB010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24912_length_3742_cov_5.324654"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PKN92_12815"
     CDS             <1..737
                     /locus_tag="PKN92_12815"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNZ49718.1"
                     /translation="KAHVDMVRACCLDGEPSPDADTVMMPASWEAALLSAGAGISAAK
                     ALLKGEIDNAFIAMRPPGHHAEAEGVMGFCLFNNIAITAHWLRASGAAERVAILDFDV
                     HHGNGTQHAFYDDDTVLFVSMHEYPHFPGTGFPYERGKNDSNLNYQMPPGSAPERWIS
                     TLEEAILPRLQAFGPDFLLLSAGFDAHRLDPLGNQLLETEHFAQITRLLRPIAGGRII
                     SFLEGGYHLGALGASAAAHIRVLMED"
     gene            complement(741..1718)
                     /locus_tag="PKN92_12820"
     CDS             complement(741..1718)
                     /locus_tag="PKN92_12820"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNZ49719.1"
                     /translation="MMRFFNIALAASLLLIVLAFHSYAEDTQTYAERLGFPKGAKVVI
                     FHNDDAGLCHEANLASIRGREEGMITSWSLMMPCPWVTEIRDYLKKNPDTCCGIHLTL
                     TSEWDHYRWRPVSCTDTCPGLADEDGYLWDNVRLVTEHATPDEFEAEVRAQIALAEKM
                     GIQISHLDTHMGTVFATEEYFQRYVAVGIDKQIPILLPAGHMTLLREEEPKFNDQAEK
                     VRALGSLLWNSGLAVIDDAHTNAYDWKKTDKREEFTQLIKSLHPGITHVIIHATVPSE
                     NFDAISGSGITRAGDFNAMLDPALKALIKEEGIILSSWKELQERRNALK"
     gene            complement(1819..>3742)
                     /locus_tag="PKN92_12825"
     CDS             complement(1819..>3742)
                     /locus_tag="PKN92_12825"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNZ49720.1"
                     /translation="DHVAMICTVNTFQARSTVRETARVMGYKEEEISALTRTLPHYGA
                     ENLSLIISALPECRHLRPEEEPLKSILKISEKIAGLPRHISIHAGGMVVTPEPLTHYL
                     PLEQAPKGMVITQYDKDAVEQMGLIKMDLLGHRALSAIRDTVQDIRAHRDPDFDIEKI
                     PHTDPATACFLRTGDTVGCFQIESPAMRGLLRKLNAQDCNALIQGIALVRPGASGSGM
                     KQHFIDRRLNREKVSCPHPFMEEALRDTHGVMIYQEDVLKVAHAVAGMSLEEADGLRR
                     AMSKKRSPREMGKSMKRFLEGAAQRGVVEEEASAVWELIANFASYAYCKAHAATYGEL
                     AYQCAYLKTHYPAEYFSAVLANGGGFYPAPVYIDEARRQGVKLLPPDVNRSGFSYQRE
                     GDGIRIGLIQIAQVTEKTARTTAQEREENGTFTTIDDFIRRVRPGRGEADLLCNAGAL
                     DSLVSRETNQPLPRPVQIWSLQQRSLCDDAALFSEPIPFFSQMPDHTQRIQTDKERNS
                     LGFPLRTPLLRYHAAVCWTRNPVASSCLAAYEGKTVCVLGTIIAVRRLSLHDGKGCMK
                     FLSLEDGWGVFEAVLFSESYQRLGHLAIPGNVCLCTGTVQREQDNITLLVSHMEKTAL
                     DSAPEILYTEVTEKTS"
BASE COUNT          824 a          995 c         1019 g          904 t
ORIGIN      
        1 caaaagccca tgtggatatg gtgcgcgcct gctgtcttga cggcgagccg agccctgatg
       61 cggatacagt gatgatgcct gcttcctggg aagcagcatt gctgtcggca ggtgccggaa
      121 tttcagcggc aaaagcattg ctgaaaggtg aaatcgacaa tgcctttatt gccatgcggc
      181 ctccggggca ccacgccgaa gcagaaggcg tgatggggtt ctgccttttc aataatattg
      241 ccattacggc acactggctc cgtgcttcag gtgcagcgga acgggtcgcc attctcgatt
      301 tcgacgtgca tcacggtaat ggcacgcaac atgcttttta tgacgatgac acagttctgt
      361 ttgtcagcat gcatgaatat ccccatttcc ccgggacagg cttcccttat gaacgcggga
      421 aaaacgacag caatctgaac taccagatgc caccgggcag cgctccggaa agatggatca
      481 gtacactgga agaggccatt cttccccggc tgcaggcctt cggtccggat ttcctgctgc
      541 tttcagcagg ctttgatgcg caccggctgg atcctttggg caatcagctg ctggagacgg
      601 agcattttgc acaaataacc cgactgttgc gccccatcgc aggagggcgc attatttcct
      661 ttctggaagg cggctaccat cttggtgccc tgggcgcaag cgctgccgct catatccggg
      721 tgctgatgga ggattgagtt ctatttgaga gcgttgcgcc gttcctgcag ctccttccag
      781 gaactcagaa tgatgccttc ctctttaatc aatgctttca gtgcggggtc aagcattgca
      841 ttgaaatctc cggcacgggt gatgccggat ccggaaatag catcgaaatt ttcagacggt
      901 accgtggcat gaatgatgac atgggtgatg ccgggatgaa gcgacttaat gagctgcgtg
      961 aactcttcgc gcttgtccgt tttcttccag tcgtatgcat ttgtgtgggc atcgtcaata
     1021 actgccagcc ctgagttcca gagcaggctg ccgagcgccc ggactttttc cgcctgatcg
     1081 ttaaacttgg gctcttcttc ccggagcagt gtcatatgcc cggcggggag gagtatgggg
     1141 atttgtttgt cgatacctac cgccacatag cgctggaaat attcttctgt ggcaaaaaca
     1201 gtgcccatgt gcgtgtccag atgtgatatc tgtatcccca ttttttccgc cagtgcgatc
     1261 tgggcgcgta cttccgcttc aaactcatcg ggagtcgcat gctcagtgac cagccgcaca
     1321 ttgtcccaga ggtatccgtc ttcatcagca agcccggggc aggtatcagt gcaggaaacg
     1381 gggcgccacc ggtagtgatc ccactcactg gtaagggtca gatgaatacc gcagcaggta
     1441 tcaggattct ttttgagata gtccctgatc tcggttaccc aggggcaggg catcataagg
     1501 ctccaggaag tgatcatgcc ctcttcacga ccgcgaatgg aagccaggtt ggcttcatgg
     1561 cagagaccgg catcgtcatt atgaaaaatc accactttgg cgcctttggg aaagcccagg
     1621 cgctcggcat acgtttgcgt gtcctcagcg tagctgtgga aagcaagcac aatgagaaga
     1681 agggaggcag caagagcgat attaaaaaag cgcatcattt cagttctttc cagggttcgg
     1741 cggcagtgaa taaactgccg ggctatgatt gtgttcctgt ataacccctc cgcagggaat
     1801 ccggaaaggg gcaaagcatc aggacgtctt ctctgtcact tcagtataaa gaatttccgg
     1861 ggctgaatcc aaagccgttt tttccatatg cgaaacaagc agcgtaatat tgtcctgctc
     1921 ccgttgtact gttcctgtgc agaggcatac atttcccgga atggcaagat gtcccagccg
     1981 ttgataggac tctgaaaaaa gaacggcttc aaaaacgccc cagccgtctt caagactcag
     2041 aaatttcatg cacccttttc catcatgaag ggaaaggcgc ctcacagcga taatggttcc
     2101 cagcacacac accgttttgc cttcgtaggc ggcaagacag ctgcttgcaa cgggatttcg
     2161 tgtccagcag acagcggcat gatatcgcag aagaggtgta cggagaggaa acccgaggct
     2221 gttccgctcc ttatcggtct gtatccgctg ggtgtgatcc ggcatttggg aaaagaacgg
     2281 aatcggttct gaaaaaaggg cggcgtcatc gcaaaggctt ctctgctgca ggctccatat
     2341 ttgtacaggg cgggggaggg gctgattcgt ttcccgggaa accagactat ccaacgctcc
     2401 ggcattgcat aaaagatccg cttctccgcg gcccggtctg acacggcgta tgaagtcgtc
     2461 aatagttgtg aaagtaccgt tctcctctct ttcctgtgcc gtcgtccggg cggttttttc
     2521 agtaacctgg gctatctgaa tcaggccgat ccggatgccg tcgccttccc gttgatagga
     2581 aaaaccgctc ctgtttacat cgggaggaag cagtttcacg ccctgccgcc gtgcttcatc
     2641 aatataaacg ggcgcaggat agaagccacc gccattggca agaacggcgc tgaagtattc
     2701 cgccgggtaa tgtgttttta agtaggcaca ctgatacgcc agctccccat aagtggcagc
     2761 gtgggctttg cagtaggcat atgaagcaaa attggcgatc agctcccaga cagcggaggc
     2821 ttcttcttcc acaacgcccc gttgtgccgc cccttccaga aaacgcttca tcgactttcc
     2881 catttcccgg ggacttcttt ttttgctcat ggcacgccgg agtccgtcag cttcttccaa
     2941 tgacatgccg gcgacagcgt gcgcgacttt cagaacatct tcctgataga tcataacgcc
     3001 atgagtgtcc cggagtgcct cttccataaa gggatgaggg caggacactt tttcccgatt
     3061 cagccttcgg tcgataaaat gctgtttcat tccgcttcct gatgcgcccg ggcgtaccag
     3121 tgcaatgccc tgaatcagcg cattgcagtc ctgagcattt aacttgcgca gcagcccgcg
     3181 catggcaggc gattctatct gaaaacatcc gacggtatcc ccggtacgga gaaagcaggc
     3241 tgttgccggg tctgtatggg ggattttttc aatgtcaaag tccggatccc gatgtgcccg
     3301 tatatcctgc accgtgtccc ggatcgcgct gagagcgcgg tgccccagaa gatccatttt
     3361 aatcagcccc atctgttcaa cagcatcctt gtcatactgc gtgatcacca tgcctttcgg
     3421 cgcctgctcc agaggcagat aatgagtcag cggttccggg gttaccacca tgcctcctgc
     3481 atggatggag atgtggcggg gaagtcccgc tattttttcg ctgatcttca ggatgctttt
     3541 caggggctct tcctccgggc gcagatgacg gcattccgga agggcgctga tgatcagaga
     3601 gagattttca gcaccataat gaggaagagt gcgggtaagt gcggaaatct cttcttcctt
     3661 atagcccatg acacgtgccg tttctcttac agtggagcgt gcctgaaagg tattgactgt
     3721 gcagatcatg gcgacatgat cg
//
LOCUS       DAOPWB010000211         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_25717_length_3657_cov_5.233759, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000211 DAOPWB010000000
VERSION     DAOPWB010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25717_length_3657_cov_5.233759"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1036
                     /locus_tag="PKN92_12830"
     CDS             368..1036
                     /locus_tag="PKN92_12830"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNZ49721.1"
                     /translation="MNSPRRGQSTKEAILEHACKIFAEKGYRGATHAEICAAAGANVA
                     SINYYYESKENLYKAAFDHLTRRMERLYPLDGGLPDTAPPEARFHAFVHAQIQRLFDP
                     DNLGARHRIRLLEVMDPTGLLEPTLSRQLEQNRKVIQHILRDLFGAPVPQRMLDWCEM
                     SIVGQCHIAFPGPGACGGPRKIFGLDASSVDALTEHIVRFSLAGIHELRRIHEKKEDT
                     SECD"
     gene            1104..2264
                     /locus_tag="PKN92_12835"
     CDS             1104..2264
                     /locus_tag="PKN92_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNZ49722.1"
                     /translation="MSSLQCTRVLLVGLLLSCTAFSQGPPGQRPTPKVSVAATETKTV
                     PVQYEYVGLTAPSKTVEVRARIQGFLDSRDFQEGSYVKKGTRLFTIEDSNYLSDREVA
                     AAQVEQAEARLHLSEQEVNRLRSVTQPGAITQRDLDQKVAEYQSASAALRLARAQLAK
                     AELQLSYTKVDAPLTGYVGKTLKEPGSFVDAAQNSLLTIMQQVDPLYVTFKVTESDFI
                     SWKHDEADGTLRRIDGDNQMRVEITLLDGRPFSSQGFLDFEDVNLDTKTGTVELRATF
                     ENKDRTLKPGQFVKVLLNGWERPDSLVVPQRAVGQAPQGAYVYVLDAENKVESRPVVT
                     GAWSGSDWVILEGLSAGERVIVNGRTKVRPGMQVEVEDPSAAPASHGPNSKR"
     gene            2282..>3657
                     /locus_tag="PKN92_12840"
     CDS             2282..>3657
                     /locus_tag="PKN92_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ49723.1"
                     /translation="MIAQFFVDRPVFAWVISIVIILGGLVSMVTLSIESSPQITPPTV
                     VISAFYPGADAETVMECVATPIEQQLSGAPNLLYYQSKSANDGSLSITLSFEIGTDLD
                     IAAVEVQNRLKRAEPSLPQEVIRQGTSVSKRMNTFLGIIALQSTNPDHDALFLSNYAM
                     IYMLDTLKRVPGVGQVMVFGNKDYSMRISVNPDLLVAKGLTVTDVAQAIREQNGLYAA
                     GEIGGRPNTEDVEFTFPVLAPGRLTTPQEFEDIILVAREDGAVVRLKDVASVQLGAQG
                     YSSEGRFRGETTAILPVIMQPLENALETMEGVKAALAELSKSFPEGMHYTIPMDTTRF
                     IKVSLWEVAMTFFQSTGLVVLVVLMFLGSWRASIVPLVAIPISIVGTFMGLLVLGFTI
                     NTLTLFALVLAIGIVVDNAIVAVENVERIMEDEHLAPRAATIKAMKQVTGPIIASVLV
                     LAAVYVPVA"
BASE COUNT          790 a          989 c         1021 g          857 t
ORIGIN      
        1 ctggaagaga ggcaggtatg acggggggag gcaggtatga cgggggagac ggggatgatc
       61 ccgaagacat gcaattcttc ccctgggaac gccaatcgac ctattggcac acacgcgggt
      121 caggacgaag acagtcactc gaatacgtaa cggaattatg cccagcgaca tattacctca
      181 gtcatttctt tgcacgccgg caattgccct gccgcccgat atttcttcgc atttatctcc
      241 agatctattg aaatccccgg gaaaatttta gatggcctag ttcgctttct tgcaatgggc
      301 taatctgatt ttctataatt caaatgcata tttgaaatga ttatttgatt ccccggaaaa
      361 tccgtacatg aattctcccc gacgcggaca gagtaccaaa gaagccattc tggagcatgc
      421 ctgcaaaatt tttgcggaaa aaggctaccg gggtgcgacc catgcggaaa tttgcgctgc
      481 cgccggcgcc aatgtcgctt cgatcaatta ttattatgaa tcgaaggaga atttatataa
      541 agccgctttt gatcatctga cccgacgtat ggaacggctg taccctctgg atggcggtct
      601 tcctgacacg gcccctcccg aggcgcgttt ccatgctttt gtccatgccc agatacagcg
      661 ccttttcgat cccgacaacc tcggtgcccg tcaccgtatc cgcctgctgg aagtcatgga
      721 tccaacgggt ctgctcgaac cgacgctcag ccgtcagctg gagcagaacc gcaaggtaat
      781 acagcatatt ctgcgtgatc tttttggtgc ccctgtgcct cagcggatgc tggactggtg
      841 cgaaatgagt attgtcggcc aatgccatat cgcctttccc ggtccgggtg cctgcggagg
      901 tcctcgaaag atctttggcc tggatgcctc ctccgtcgat gctctgactg agcacattgt
      961 ccgtttttcg ctggccggta ttcatgaact tcgccgtata cacgagaaaa aggaggacac
     1021 atcagagtgc gactaacggc tctgcccccc gctttccttt ttctgtcatt ctgtaagtcg
     1081 tccctatcat caggagtgtt tcgatgtcat cccttcaatg tacccgagtc ttactggtcg
     1141 gcctgctttt atcctgtacc gctttctcgc agggtcctcc gggtcagcga ccgacaccca
     1201 aagtgagtgt tgccgctacc gagacaaaaa cggtgccggt tcagtatgaa tatgtagggc
     1261 ttaccgcgcc gtcgaaaaca gtggaagtcc gtgctaggat acagggattt ctggacagcc
     1321 gtgatttcca ggaaggctcc tatgttaaaa aaggtacccg cctttttacc atagaagaca
     1381 gtaattatct gtccgatcgg gaggtggcgg cggcgcaggt tgagcaggcg gaagcacgtc
     1441 tgcacctgag cgagcaggaa gtgaaccgcc ttcgctcggt gacccagccc ggcgccatca
     1501 cacagcgcga tctggatcag aaagtggcgg aataccagag cgccagcgcg gcgcttcgtc
     1561 ttgcccgtgc ccagcttgcc aaagcggaac ttcagctgag ctacacgaaa gtggatgcgc
     1621 cgctcactgg ttatgtgggc aaaacgctga aagagccggg cagtttcgtg gatgccgccc
     1681 agaacagtct gctcacgatc atgcagcagg tggatccgct gtatgtgact tttaaggtaa
     1741 cggaaagcga tttcatctcc tggaaacatg atgaagcgga cggtaccctc cgccgcatcg
     1801 atggggataa tcaaatgcgg gtggaaatta cccttctgga cggccgccct ttcagctccc
     1861 agggttttct cgatttcgaa gatgtcaatc tggatacgaa aacaggaacc gtggagctgc
     1921 gcgccacttt tgaaaacaaa gaccgcacct tgaagccggg gcagtttgtg aaggtactgc
     1981 tgaacggatg ggaacgcccc gattcattgg tggttcccca gcgtgcggtg ggtcaggcgc
     2041 cacagggcgc ttatgtctat gtgctggacg cggaaaacaa agtagagtcc cggcctgtgg
     2101 tcaccggtgc ctggtccggt tccgactggg tgatcctcga agggctgtca gcgggagagc
     2161 gggtcattgt caacggcaga acgaaggtgc gcccgggcat gcaggtggaa gtggaagatc
     2221 cgtcagcggc acccgcttct cacggaccga attccaaaag ataaccggaa caggattcat
     2281 tatgatagcc caattctttg ttgaccggcc tgtcttcgcc tgggtgattt ccattgtgat
     2341 cattctgggc ggcctggtat ccatggtcac cttatccatt gaatcatctc cccagattac
     2401 gccgcccacc gtagtgatca gcgcatttta tccgggtgcc gatgcggaaa cagtgatgga
     2461 atgcgtggcg acgcccatcg agcagcagct gagcggtgcc ccgaaccttc tctattacca
     2521 atcgaaaagt gccaatgacg gcagcctctc gatcactctg tctttcgaaa tcggaacaga
     2581 tttggacatt gccgccgtgg aagtgcagaa ccgtctgaaa cgggcggagc ccagccttcc
     2641 gcaggaagta attcggcagg gcacttcggt ttccaagcgc atgaacacct ttctgggcat
     2701 tattgcgctg cagtcgacca atcccgatca tgatgcgctt ttcctgagta actatgccat
     2761 gatctacatg ctggacacgc tgaagcgtgt tcccggtgtc ggtcaggtga tggttttcgg
     2821 caacaaggat tattccatgc ggatttccgt caatccggat ctgctggttg ccaaaggtct
     2881 taccgtgacc gatgtcgcac aggcgatccg tgagcagaac gggctgtatg ccgctggaga
     2941 gattggcgga cggcccaata cggaggatgt ggaatttacc tttcccgtgc tggcgcccgg
     3001 ccgtcttacg acccctcagg aatttgaaga catcatcctg gtcgcccggg aagacggcgc
     3061 tgtggtgcgg ctgaaagatg tcgcttccgt acagctcggt gcgcagggct acagctctga
     3121 aggccgtttc cgcggagaaa ctacagccat attgcctgtc atcatgcagc cgctggaaaa
     3181 tgcactggag accatggaag gcgtgaaagc ggcgctggca gaactttcca agtcttttcc
     3241 cgaaggcatg cattatacca tccccatgga tacgacccgc ttcatcaaag tatccctctg
     3301 ggaagtggcg atgacctttt ttcagtccac cgggctggtg gtgctggtgg tgctgatgtt
     3361 tctcggcagc tggcgggcat ccattgtgcc tctcgtcgcc attcccattt ccattgtcgg
     3421 cacctttatg gggctgcttg tgcttggctt caccatcaac acgctgaccc tgttcgcgct
     3481 ggtacttgcc atcggcattg tggtggacaa tgccattgtc gccgttgaaa acgtggagcg
     3541 catcatggag gatgagcatc tggctccccg tgccgcaacc atcaaagcga tgaagcaggt
     3601 gacagggccc atcattgcca gtgtgctggt gctggcggcg gtttatgtgc cggtcgc
//
LOCUS       DAOPWB010000212         3558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_26719_length_3558_cov_4.956323, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000212 DAOPWB010000000
VERSION     DAOPWB010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26719_length_3558_cov_4.956323"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..3183)
                     /locus_tag="PKN92_12845"
     CDS             complement(214..3183)
                     /locus_tag="PKN92_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49724.1"
                     /translation="MSFSVKSFFPFVFLSIVGLCAAGFATAEAEFSVPETVYQGELAS
                     FPGAFAFQIPAPGIILINDEQLRQLAEGDTPVDLSLTYTKRVESLRQICEQAQAAGYR
                     TLILSFDYFFTQYREGGQTAPRLLTPDRPEYVELIAKISRFVQNYGLGLELSLLSPLE
                     IGNGYREQTGESGRWMHFRKGLRDPESGEYSVELWRQRRWANNKGVVNIEDAGIRVFA
                     FQEHTVSQSPYKVVPPDSIVDISDTASVQVYEHNINRSGDYEAVRIRVHGSGGVPGKN
                     RVLVVQHYTTPEMDYFSDSASAYLKKLGDCYADAGVVLNGLYSDEMHIQQDWNYFGHH
                     DHGQFAVRYVSKGFEKRFAELYGEEYKDFAKYMIYFVQGQEDAANNLTATLEIQHVFG
                     ASPEDIRRTALFRSNYYRLIQNGVVDLFTGAKKHLEMRMGHLLQARAHATWAESPTID
                     HWYVEARPHPQNQYEYTSNFLWSCTVHQAASACHDYFKWNDFLTGGGNDHTEGGWLDR
                     DYFGAAIACSTGILNRVPYAYAAHWGMPGAISERRMAVIEAFGASGRPMYGMVQDMEH
                     RDVDVLMLYPLDLVAANERFGSWMTQYAYANYVTQEKLLEEGRVEDGAIILGGRKFTT
                     LVTLFEPFPDKKLLRWMEELASGGGTVIWSGPPPVVTRDGAPARDQWNALTGAGYEPT
                     VQEGLLAPGRQIRFSGALGHLPEMTVLTDFTVDRIYPVQPSDGTEVLAREGGHIVGTR
                     RHLGTGSVSVLAFRPRDDQSRSLGYETRFWFEILHALGAYPATGIFGDQNDNTEYLSR
                     TTPYICCRFPNGALAVAPHLRELEECWPGGFARKEEEDQKLMERLALPPNTLHLDHFK
                     IDGREISYDGMNAMTFRADASGKLLAFAGQYTQAITVDGTTTQFADQPMPLIAWAPLR
                     QDRKVPGGASMLLYYGGAGTLRLPCSTGVRSVEAYAEGATPASRGSRLDARVEEGNIV
                     IAADAAQYRWIYICEEE"
     gene            complement(3418..>3558)
                     /locus_tag="PKN92_12850"
     CDS             complement(3418..>3558)
                     /locus_tag="PKN92_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49725.1"
                     /translation="LFNHVPGGSNVLYMDGHVEFQRYEEKGKSFANRRVAETLGVMAA
                     AL"
BASE COUNT          889 a         1012 c          874 g          783 t
ORIGIN      
        1 ggtggtgcga caggcggcgg tggcgccgat gtgaatgtcg cccaatactg atctgtattt
       61 tctctacgtt atgaattata aatacccggg cccctttcgg ggttcgggta ttttgtgttt
      121 ttttgtatcc cttcagtaaa gggacatcac ctcaaaggat gcaccgccac aggagaaggg
      181 gatgcgcctg taccggtttt cagtgtggtg cactcattct tcttcgcaga tataaatcca
      241 gcggtactga gcggcgtcag cggcaatcac aatattccct tcctcaacac gggcatccag
      301 gcggctgccc cggctcgccg gcgtcgcccc ttccgcatag gcttccacac tgcgaacacc
      361 ggtactacac ggcagccgca gtgtacccgc accaccataa taaagaagca tcgatgcgcc
      421 cccgggtact ttgcgatcct gtcttaaagg tgcccaggcg atgagaggca tcggctggtc
      481 ggcaaactgt gttgttgtgc cgtccaccgt tattgcctgt gtatactgtc ccgcaaaagc
      541 aagcagcttc ccggatgcat ctgctctgaa cgtcatggcg ttcatgccat cgtaagaaat
      601 ttctctgcca tcgattttaa aatgatccag gtgtagtgta ttgggcggca gcgccaggcg
      661 ctccatcagc ttctgatcct cttcctcctt gcgtgcaaaa ccgcccggcc agcattcctc
      721 cagctcacgc aggtgaggcg ccacagccag cgcaccattg ggaaaacggc agcagatgta
      781 tggtgttgta cgggaaaggt attcggtgtt gtcgttctga tccccaaaaa tgcccgtagc
      841 cggataggca ccgagtgcat gcagtatttc aaaccagaag cgcgtttcat acccgagact
      901 gcgggactga tcatcccggg gcctgaacgc taagacggat acagatcctg tgccaaggtg
      961 ccgccgtgta ccaacaatat gtccgccttc acgggcaagc acttccgtac cgtctgaagg
     1021 ctgcacagga taaatacgat caacagtgaa atccgtcaga acagtcattt caggcaagtg
     1081 ccccagcgca ccggaaaaac ggatctgccg cccgggcgcc agcagccctt cctgtacagt
     1141 aggctcgtaa cccgcaccgg tcagcgcatt ccactgatcc cgggcaggcg caccatctct
     1201 ggtgactaca ggaggcggcc ccgaccagat cacagtgcca ccgcccgatg ccagttcttc
     1261 catccagcgc agcagtttct tgtcaggaaa aggctcgaac aaggtgacca gtgtcgtaaa
     1321 ttttctgccg cccagaataa tggcaccatc ctccaccctc ccttcctcca gcagtttttc
     1381 ctgcgttaca tagttggcat aggcatactg agtcatccag ctgccgaagc gctcattggc
     1441 ggcaacaaga tccagcggat aaagcatcag cacatccaca tcgcgatgct ccatatcctg
     1501 gaccattccg tacataggtc tgccggaagc gccgaacgcc tcgatcacag ccatgcgccg
     1561 ttcgctgatc gctccgggca taccccagtg agcggcatag gcatagggca cacggttgag
     1621 aatccctgtg gaacaggcga ttgcagctcc gaagtagtcc ctgtccagcc atccgccttc
     1681 cgtatgatca ttacccccgc cggtaagaaa atcattccac ttgaaataat catggcacgc
     1741 cgacgccgcc tgatgcaccg tgcaggacca gaggaaattg gaggtgtatt cgtactggtt
     1801 ctgaggatga ggccgagctt ccacatacca atgatcaatg gtggggctct ccgcccaggt
     1861 cgcatgggca cgcgcctgaa gcaaatgtcc catccgcatt tcaagatgtt ttttcgctcc
     1921 ggtaaaaaga tccacaacac cattctgaat cagccggtaa taattcgatc gaaaaagagc
     1981 ggtgcgccgt atatcttcag gagacgcacc aaatacatgc tgaatctcca gtgttgccgt
     2041 cagattgttt gccgcatcct cctgaccctg cacaaaataa atcatatatt ttgcaaagtc
     2101 tttatactcc tcgccatata gttcggcaaa gcgcttttca aaccccttgc tcacataacg
     2161 cacagcaaac tgaccgtgat catgatgtcc gaaataattc cagtcctgct gaatatgcat
     2221 ttcatcgctg taaagaccat tcagcaccac gcctgcatcc gcatagcagt cgcccaactt
     2281 tttcagatag gccgatgcac tgtcgctgaa gtaatccatt tccggggtgg tatagtgctg
     2341 caccaccagg acacgatttt tgcccggcac accgccgctg ccatgaaccc ggatgcgcac
     2401 cgcttcataa tcgcccgaac ggttgatgtt gtgctcataa acctgcaccg atgcggtatc
     2461 ggaaatatct acaatactgt ctggaggaac gaccttgtaa ggcgactggg aaaccgtatg
     2521 ctcctggaaa gcaaaaacac ggataccggc gtcctcaatg tttaccacac ctttgttgtt
     2581 tgcccaccgc cgctgccgcc agagctccac actgtattca ccggattcag ggtcacgaag
     2641 tccttttctg aaatgcatcc accgccctga ctcgcctgtc tgctcacgat acccgttccc
     2701 aatttccaga ggcgacagaa gactgagttc aagacccaga ccgtagttct gaacaaagcg
     2761 gctgatcttg gcaatcagct ccacatattc cggtctgtcc ggcgtcagca ggcggggtgc
     2821 agtctgtccg ccttcccggt actgggtgaa aaaataatcg aaggaaagga ttaaagtcct
     2881 gtatcctgct gcctgtgcct gctcacaaat ctgacgcaga ctctcaacac gctttgtgta
     2941 ggtcagactg agatccaccg gtgtatcccc ttctgccagc tggcgcagct gttcatcatt
     3001 aatcaggata atgccgggcg caggaatctg gaatgcaaaa gcgccgggaa aagaagcaag
     3061 ttctccctga tacacagttt cgggtactga aaattccgct tcagcagtgg caaacccggc
     3121 agcacacaaa ccaacaatcg aaagaaaaac aaacgggaag aaggatttga ccgaaaaaga
     3181 cataataaac agattccttt cacgtgatat caagagtctg gtgcttccag agcaagtaac
     3241 cgggtggcac aattttaacg ggacaaagct gaatatatat caggctagca taaccggcaa
     3301 taaaaaagtt aatgcgagga ggtaccggtt tcgggaggga acagggcata aaaaagccgc
     3361 gccagaacga agcctggcgc ggacaagtca aatcaggtta aaaaatgtgt cttacgatta
     3421 aagggctgct gccatcacac ccagtgtctc ggcaacacga cgattggcaa aggattttcc
     3481 tttttcttcg tagcgctgga attcaacgtg accatccatg taaagcacgt tggagccgcc
     3541 gggaacgtgg ttgaaaag
//
LOCUS       DAOPWB010000213         3408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_28319_length_3408_cov_5.660304, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000213 DAOPWB010000000
VERSION     DAOPWB010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28319_length_3408_cov_5.660304"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PKN92_12855"
     CDS             complement(<1..1163)
                     /locus_tag="PKN92_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ49726.1"
                     /translation="MNVSQIVDALKTDEHFCANLTAWRSFSERPAQYSDFPETVHPEL
                     IDGFRKRGIARLYSHQAEAFSAIEAGENVCVVTPTASGKTLCYNLPVLNQILRHQESR
                     ALYLFPTKALSQDQVTELTETIETLGVKIGAYTFDGDTPSSARKAVRNAGHVIVTNPD
                     MLHAGILPHHTIWVRLFENLKYVVIDEIHQYRGVFGSHLTNVIRRLKRLCEFYGAKPQ
                     FICCSATIANPKELAERLIEAPARLIDNNGAPAGEKHFLFYNPPVVNRELGIRASSVK
                     QAGRLASSLLFKGVQSIVFARSRLRVEILTTYLKEAVARRGGDADKVRGYRGGYLPTE
                     RREIEQGLRNGEVMAVVSTNALELGIDIGSLDVCIMCGYAGGISRTWQQAGRAG"
     gene            1479..2942
                     /locus_tag="PKN92_12860"
     CDS             1479..2942
                     /locus_tag="PKN92_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49727.1"
                     /translation="MTCKSMRFRPLFSAILCLALCSVFVFTAQAASISELLGFLPEGC
                     QFAVALPNIESVEKEAAPLLQLPFASEADAFAAALGGDTFSEGLEKCGINIKAPAVLF
                     TRFDSPRDVVFGGILQVKDAAAASSAVAGLVAGSSEEVDLRGDLKANYVDSSHVGYFM
                     NGDKLFIASSLSMLNHFADRIQAPQAIRYNPGNREEVVAFTRIDLLDKSGLLDSVPQL
                     AKVKPIVDTLRPFSDELILAIGEVAGQAYLRAAAHDSSNAPVPSPGALGLHGFMNPEA
                     PLVANLRLTPELINTLSMAMVAEPATRQVGGYIRVAAGLLGDELALSFNSLKDGDVPD
                     ALIAAKVKQASTVPNLLKLIAKIDAPAYQFADNDVYVVEKVADGTDLHIAVAGDTLVV
                     TPGKEELKTSVDAFNQTSGTSGVDSGIVNQGVFGFISLDGAKAAKVLPPNLLPANLDL
                     SKISLALTLGIDGEWREMVLSSPKGFAGFASLLEDLI"
     gene            complement(3057..>3408)
                     /locus_tag="PKN92_12865"
     CDS             complement(3057..>3408)
                     /locus_tag="PKN92_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNZ49728.1"
                     /translation="GYFYIVDRKKDIIIRSGMNIYPREIEEILYGHPKVLEASVVGVP
                     DAARGEEVKAYISTIRGESVEPEEIIDYLSQRMAKYKVPREVEVLDELPKGPTGKILK
                     RELKVRAIKESSGG"
BASE COUNT          684 a          923 c          976 g          825 t
ORIGIN      
        1 cctgcccggc ccgcctgctg ccaggtacgg gagatgccgc ctgcatagcc gcacataatg
       61 cacacatcga gtgagccgat atcgatgccc agttccagcg cattggtgct gaccaccgcc
      121 atcacttcgc cgttgcgcag gccctgctcg atttcacgcc gctccgtggg caggtagccg
      181 ccccggtaac cgcgcacttt atccgcatcg ccgccccgcc gtgccaccgc ttctttcagg
      241 taggtggtga gaatttccac gcgcagccgg cttcgcgcaa agacaatgga ctgcactcct
      301 ttgaagagca gcgatgaggc gaggcgtcct gcctgtttca cgctggacgc acggattccc
      361 agctcccggt tgactacagg cgggttatag aaaaggaaat gcttttctcc ggcaggggcg
      421 ccattgttat cgatcagccg ggccggcgcc tcgatcagtc tttccgccag ttccttcgga
      481 ttggcgatgg tagcgctgca gcagatgaat tggggctttg cgccgtaaaa ttcgcaaagc
      541 cgtttcagcc gccggatcac attggtcaga tgactgccga atacgccccg gtactggtga
      601 atttcatcga taaccacata tttaaggttt tcgaaaaggc gtacccagat ggtgtgatgc
      661 gggaggattc cggcatgaag catatcgggg ttggtcacaa tcacatgccc ggcattgcgc
      721 accgctttcc gtgccgagga gggagtgtct ccgtcaaagg tgtaggcgcc gattttcaca
      781 ccgagcgttt caatcgtttc cgtcagttcc gtgacctgat cctggctcag tgctttggtc
      841 ggaaaaaggt aaagtgccct gctctcctga tggcgaagga tctgattgag gacaggcagg
      901 ttgtagcaca aggttttgcc cgaagcggtc ggcgtgacaa cgcacacgtt ttctcctgct
      961 tcgatagcgg aaaaggcttc cgcctgatgg ctgtacagcc gggcaatgcc ccgttttctg
     1021 aagccgtcaa tcagctccgg atgcaccgtc tccggaaagt cgctgtattg cgccggccgt
     1081 tcagaaaagc tgcgccaggc ggtcagattc gcacaaaaat gttcgtccgt ttttaaggcg
     1141 tctacaatct gggacacatt catggaaatt ctttctgagc tggtcataaa agacgcacaa
     1201 ggccgctgcg ctccgcacac cacagcgtac ctgtctattg tacagatttc ggcaggtcgg
     1261 tcaacaatcg gaaggcaaag aaataatata agggatgaaa ccttgtccgg gatttctgtg
     1321 tctaacgtcg gcagtcagta tttgtcggct gccgaagatt ctcttgtaaa ggcccggcga
     1381 gcgcccggct attgataagt aggctttgtg gtactgcgga aaggtgcttt ttgccagtac
     1441 gaaccagtta acgttaactt taggatggag aaaatgaaat gacctgtaaa tcaatgcgat
     1501 tccgcccgct cttttcagca atcctttgtc tggctttgtg cagcgttttt gtttttacgg
     1561 cgcaggcagc gtctatttcc gagctgctgg gctttttgcc tgaaggctgc caatttgcgg
     1621 tggcactgcc caatattgag agcgttgaaa aagaagcggc accgctgctg cagctgcctt
     1681 ttgcatctga agcggatgcc tttgcggcgg cactgggcgg cgacactttc tcggaagggc
     1741 ttgagaaatg tggcatcaat attaaggcgc ctgccgtcct gttcacccgt tttgactcgc
     1801 cccgggacgt tgtgttcggc ggtattcttc aggtgaaaga tgcagccgcc gccagttccg
     1861 ctgtggcggg gctcgttgcc ggttcatcgg aagaggtgga cctccgggga gacctcaagg
     1921 cgaactatgt ggactccagt catgtgggtt atttcatgaa cggggataaa ctctttattg
     1981 cttcgtccct ttccatgctg aaccactttg ccgaccgcat tcaagcgcct caggcaatcc
     2041 gatacaatcc cggcaaccgg gaggaagtag tggctttcac gcgtatcgat ctgctggaca
     2101 agagcggact ccttgattcc gttccgcagc tggcgaaggt taagcctatt gttgacacgc
     2161 tccgtccttt ctccgatgaa ctgattcttg ccataggcga agtggcgggc caagcctatc
     2221 tccgtgccgc cgcccatgat tcgagcaatg cacccgtgcc ttcacccggg gcgttgggac
     2281 tgcatggctt catgaatccc gaggcgccgc tggtggcgaa tctccgcctg acaccggagc
     2341 tgatcaatac gctcagcatg gcgatggttg cagagcctgc cacccgtcag gtgggcggtt
     2401 atatccgtgt tgccgccgga ctgctggggg acgagcttgc gctgagtttc aacagcctga
     2461 aggatgggga tgttcccgat gcactgattg ccgccaaggt gaaacaggct tcgaccgtgc
     2521 cgaacctgct gaagctgatt gcgaaaatcg atgcccctgc ctatcagttt gccgacaatg
     2581 acgtgtatgt ggttgagaaa gtggcggacg gtacggatct gcatattgcc gtcgccggtg
     2641 acaccctggt ggtgaccccc ggtaaagagg agctgaaaac gtcggtggat gccttcaatc
     2701 agacttccgg cacctcgggc gtggacagcg gtatcgtgaa tcagggcgtc ttcggcttta
     2761 tttcgctgga cggagcaaag gccgcaaaag tacttcctcc gaatcttctg cccgccaatc
     2821 tcgatctttc caaaatcagt ctggcactga cgctgggcat cgatggcgaa tggcgcgaga
     2881 tggtgctgag ttctccaaag ggctttgccg ggttcgcctc cctgctggaa gatctgatct
     2941 gatctgacct gatcctgttt tgcttcagcc gccattccgc accgggatgg cggctttttt
     3001 agtgcaggcg ttcatctctg aagcgggata gaaatctgaa gcgggataga aagggctcag
     3061 ccgccgctgc tttctttgat ggcgcggact ttgagttccc gtttcaggat tttgccggtg
     3121 ggacctttcg gtaattcatc gagcacttcc acttcacgcg ggactttgta tttcgccatg
     3181 cgctgggaga gatagtcgat aatttcttcg ggctcaactg attcgccacg gatggtggaa
     3241 atataggctt tgacctcttc gccccgggcg gcatcgggca cgccgaccac ggaggcttcc
     3301 agcactttcg ggtgaccgta gaggatctcc tcgatttccc ggggataaat gttcatgccg
     3361 cttcggatga taatgtcttt tttccggtcg acaatataaa aatagccg
//
LOCUS       DAOPWB010000214         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_28386_length_3401_cov_6.714883, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000214 DAOPWB010000000
VERSION     DAOPWB010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28386_length_3401_cov_6.714883"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1482
                     /locus_tag="PKN92_12870"
     CDS             97..1482
                     /locus_tag="PKN92_12870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNZ49729.1"
                     /translation="METANTGNRWWVVVGALIIQISLGAVYIWSVFQTPLRSHFPGWT
                     ETQVTWPAQVLLAIFALAVIFGGRIQDRLGPRIVSTAGGIILGIGLILSSFTNHYSES
                     AALWWLLLTFSILGGTGIGMSYVCPIATCAKWFPDKRGMITGLAVAGFGAGAFFFAPL
                     ARALISGGYYQIFGVDLFPLPAVGVFNTFLVLGFIFLVAVVLGSQFLKNPPAGYVPAG
                     WTPPTPAASATAAKVDYTPTEMLKTPVFWLLWITYFAGCTAGLQVIMKASPIWQSFSI
                     SAMTQFPIEQHVFVNVVSAGAMVVAVLAIFNATGRIFWGKVSDSLGRKNTLILMFVIC
                     GIVMLGLDWMHSYLLYTVSACTVGLCFGGYLALYPAVTTDFYGTKHIGVNYGWMFTAY
                     GAGGIAGPFLAAWLMKAGQAVEYQVMEGGALTTKLFHVGDYRQAFMISGAACLISAVL
                     MLFVKAPHPSK"
     gene            1703..2059
                     /locus_tag="PKN92_12875"
     CDS             1703..2059
                     /locus_tag="PKN92_12875"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HNZ49730.1"
                     /translation="MGFQDFTVKVTLRAFVTVLVFSIVLMVLGLPFIAACLAGLNIVT
                     FLIYGWDKYASRRYMTRIPEITLLSLGAAGGTAGALLDQELFRHKTKSKAFRLRFFAI
                     TVAQITVLLLFCWLWL"
     gene            complement(2164..3111)
                     /locus_tag="PKN92_12880"
     CDS             complement(2164..3111)
                     /locus_tag="PKN92_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNZ49731.1"
                     /translation="MADSILTRVGIFYDGNYFLHVSNYYAFSHLRRARINVSGLHNYI
                     RRKVAECEGADERYCQIVDAHYFRGRLRAYDADERDMLLKERVFDDILSREGVTSHYL
                     PLGPFGEKGIDVWLALEAYELAIYKKFDVTVLITGDGDFLPLVRKLNTLGTRVMLLSW
                     DFKYFDKNGTERETKTSQSLLEEATYPIPMHHLIGDGALSKDPMINGLFAHKTEQAFP
                     ALSSSFQEMMPPTSAADITHHAGDTAVPQKGYIENLKEGYGFISPEDGGENLFFFHSE
                     VLNADFNDLQIDDPVSYHLGENAKGICAVRVRILEEDIN"
BASE COUNT          728 a          824 c          885 g          964 t
ORIGIN      
        1 ctcttttacg ggagccggtt tcacgagaga gaccggctcc tacctctctc actttgctgg
       61 gattattttt caacctttcg aggagaaccg atttttatgg agacagcaaa cacaggtaat
      121 cgctggtggg tagtggtagg tgcactgatc attcagatca gcctgggcgc cgtgtatatc
      181 tggagtgtgt ttcagacgcc gctgcgcagt cacttccccg gatggacaga aacgcaggtc
      241 acctggcccg ctcaggtgct gttggcgatt ttcgctctgg cggttatttt cggcgggcgt
      301 atacaggatc ggctcggccc tcgtatagtc agtacggcag gcggcattat tctgggtatc
      361 ggactgattc tttccagctt taccaatcat tattccgaga gtgcggcgct gtggtggctg
      421 ctgctcactt tttcgatcct cggcggtacg ggtatcggca tgtcctatgt atgccccatt
      481 gccacctgcg cgaaatggtt tcccgataag cgcgggatga ttaccgggct tgccgttgcc
      541 ggattcggag cgggcgcttt cttttttgcg cctcttgccc gcgcccttat ttcgggcggc
      601 tactatcaga ttttcggtgt tgatcttttc ccgctgcccg ctgtgggcgt attcaacacc
      661 tttctggtgc tgggttttat tttcctcgtt gccgtggtgc tgggttccca gttcctgaaa
      721 aatccgccgg cgggctatgt ccccgcaggc tggacaccgc caacgcctgc cgcttcagcg
      781 acagccgcca aagtggacta tacgcccacg gagatgctga agacgccggt tttctggctt
      841 ctctggatca cctattttgc gggctgcacg gcagggcttc aggtcattat gaaagcatcg
      901 cctatctggc agtcgttcag tatttccgcc atgacccagt ttcccattga gcagcatgtt
      961 tttgtgaacg tggtgtctgc aggtgccatg gtggtggcgg tgctggcgat tttcaatgcc
     1021 accggcagaa ttttctgggg taaggtctcc gattcgctgg ggcggaaaaa cacgctgatc
     1081 ctgatgtttg tgatctgcgg tattgtcatg cttgggctgg actggatgca cagctacctg
     1141 ctgtacacag tcagcgcctg tactgtcggc ctgtgctttg gaggctacct ggcgctgtat
     1201 cctgccgtga caactgactt ttacggcaca aagcatatag gcgtcaatta tggctggatg
     1261 tttacagcct atggtgccgg cggtattgcc ggtccttttc tggcggcgtg gctgatgaaa
     1321 gcgggccagg cggtggaata tcaggtgatg gaaggcggag ctctcacaac caagcttttc
     1381 cacgtgggcg attatagaca ggcttttatg atttccggcg cggcatgcct gatttccgca
     1441 gtgctgatgc tctttgtgaa agcgccccac ccttctaaat aactttctgt ccggtatcac
     1501 ctccgataca ggcgagtcaa acactgaagc ggctccagac tccggtcgga gccgtttcac
     1561 ttatttcaga gcctgaaacg aacagcaaac aaaggcagat taaagaaaga tctatagaaa
     1621 aacgtgatta aattcggtat actggcacaa gattctgaaa ataaacaagg ggctttcaat
     1681 gccggatgca ggaagggcgg ttatgggttt tcaggatttt accgttaaag tgacactgcg
     1741 ggcttttgtg actgtgctcg tattttctat tgtgctgatg gtacttggct tgccttttat
     1801 cgcagcgtgc ctggcaggtc ttaacattgt tacttttctg atttacggct gggacaaata
     1861 cgcatcccgc cgttatatga cccgtattcc cgagattacg cttttgtcgc tgggagctgc
     1921 aggaggcaca gcgggagccc ttctcgacca ggaattgttc cgtcacaaaa caaagtccaa
     1981 agcattccga ctccgctttt ttgcaattac agtggcacaa atcaccgtac ttctgctttt
     2041 ctgctggcta tggctttaaa atagcaggta cacattcaga gaatgatctt ttcctgaaag
     2101 gccactccgt cctcatgagt cctgttttga agaaataaat tcaacgttat ttaaagtccg
     2161 gcatcagttt atatcttctt ccaggatacg gacccgtacg gcgcaaatgc ctttggcatt
     2221 ttcgcccagg tgataggaga cgggatcatc aatttgcaag tcgttaaagt ccgcattcag
     2281 cacttctgaa tgaaagaaaa atagattttc tcctccgtct tccggggaga tgaacccgta
     2341 gccttctttc aggttttcaa tatatccttt ctgaggaaca gcggtgtcgc cggcatgatg
     2401 ggttatgtca gcggcggatg tcggcggcat catttcctga aatgacgagg atagagccgg
     2461 aaaagcctgc tcagttttgt gtgcgaataa gccgttaatc atgggatctt tggaaagcgc
     2521 tccgtcaccg ataagatggt gcatcggaat gggatatgtg gcttcctcca gcagagactg
     2581 cgatgttttt gtttcgcgct ctgtgccatt tttatcaaag tatttaaagt cccatgacag
     2641 cagcatgact ctggtgccca gtgtattgag ctttcgtacc agaggcagaa agtccccgtc
     2701 tccggtaatt aagacagtta catcgaattt tttatagatg gcaagctcgt acgcttccag
     2761 tgccagccat acgtctatgc ctttctctcc gaacgggccg agcggaagat aatgactggt
     2821 aacaccttcc cgactgagga tatcgtcgaa cacgcgctct ttgagcagca tatcgcgttc
     2881 atcggcatca taggcgcgca gtcgtccccg gaaataatgg gcgtccacga tctggcaata
     2941 tctttcatct gctccctcgc attccgcaac tttacgccgt atgtagttgt gcagcccgga
     3001 gacattgata cgtgcccttc gcaggtggct gaatgcgtaa tagttgctga catgcaggaa
     3061 atagtttccg tcgtaaaaaa tgcctactct ggtgagaatg ctatctgcca cggtgctgtc
     3121 cttctgttga atctttgaaa ataattatac tgttcatatg tttattctga caccgccccg
     3181 gagaactatc tccatttttt ttgttttttt caatgaggag ctatcaggtt taaggcggta
     3241 aatctaacat ttaaatcagg gtgtatagat tataatgacc cgtacgctgg cggtgctatg
     3301 tagggggttt attttttcct gaagggtcaa ctcaagggga ttataacgaa gctctcagct
     3361 gaggttagac tttcacctgt tataaaagta tacttcgtcg g
//
LOCUS       DAOPWB010000215         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_29005_length_3349_cov_4.555859, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000215 DAOPWB010000000
VERSION     DAOPWB010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29005_length_3349_cov_4.555859"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PKN92_12885"
     CDS             complement(<1..1034)
                     /locus_tag="PKN92_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ49732.1"
                     /translation="MSLVRFENVNKSFGGEPLFSDVNLRVEEGERIALIGRNGTGKTS
                     LFRLITGESEADSGTIERMRRRRIVYLSQIPEVDPASTVFDVAMEGFQDLIQEEKELH
                     RLEQRLHHDEPGLLELYGERQHAFSLAGGYEFRTRARQILFGLGFLPEDFEKPFSALS
                     GGWRARLMLSLALLREADLLLLDEPENHLDMTAREWLETHLAGRPEAMILITHDRRMV
                     NVLAHRILEIEGGAVTAFTGNYDRWLKEKNLRREQQQKAFARQESYIQKEQAWIDRFR
                     AKNTKAKQAQAKMKRLEKLERVDAPPPELATAVFHLEKAERSGDMVLDARSLTMAYGN
                     RSLYQDLSFSL"
     gene            1260..2927
                     /locus_tag="PKN92_12890"
     CDS             1260..2927
                     /locus_tag="PKN92_12890"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNZ49733.1"
                     /translation="MNIAKSVLPVFCALVVSALFSACSTEAIWVSRDQIDFDRDESPM
                     YFHVANNNAEMGTFTVNITGNKNWIKVSPQTIPCKPPTESGMVMERIEVRIDRSRITS
                     TGKHTGEIQLRASGIKTVTLKVSVVQTSVNPTLPPLSINNPVVTYKSPSLIEFAFSLR
                     DQKDRAVTGEPAQFGLQAFESRRPVGTPEGLTLRRGASRQLWLSLVMDYSQYMTEIGE
                     NAIDEMERVATEALLPSLNEDALVSVRAFYRNTENSKEIVPFTVNREFAAQEIREIRS
                     KYLPGFNSGARVYEALLAAIQRFPEEERTEKDDRYIVLFCNGRDTTGVPSMEIVREAA
                     LKKKVQIFVACLGDSMDADKLITLARSTNGRFVAADSLNTLQTAFQRIVEDLYGQYIV
                     RWASGREDTFNIIPSITLTYNGAAASYEASKAFVPSQHLGDRMRGELILVQSETPGKN
                     TTVFLRAHYVPYGISALQLRVQSSHSYDVALVDAIDDGLLAGWQLETEDTQAGEKLIR
                     ATGSASIPFAAFGAMLRFEFDEEVEDAFTSFVIENAGYVDGQRFVLM"
     gene            complement(2936..>3349)
                     /locus_tag="PKN92_12895"
     CDS             complement(2936..>3349)
                     /locus_tag="PKN92_12895"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNZ49734.1"
                     /translation="VFMEVYRKLGGAIESLTEPDAVASALQSLERKGTIVDAMLGTGA
                     KGGAQGLVLAAIQHWPEMYTLAVDVPSGLDADSGIPGGICIRADLTVTFAFAKQGFLN
                     PAAKNYLGSLVVEDIGLPDCCAVPAVLERLCIEEH"
BASE COUNT          814 a          860 c          885 g          790 t
ORIGIN      
        1 agcgaaaaag aaagatcctg atacagagag cggtttccgt atgccatggt aagcgaacgg
       61 gcatcgagaa ccatatctcc gcttcgctcc gctttttcca gatgaaaaac ggctgtggcg
      121 agctccggcg gcggtgcgtc aacgcgctcc agtttttcaa ggcgtttcat tttggcctgc
      181 gcctgtttcg ctttggtgtt tttggcacgg aagcgatcga tccacgcctg ttctttctgg
      241 atataagatt cctgccgggc gaaggccttc tgctgttgtt cccgtcgcag atttttttct
      301 ttcagccatc ggtcatagtt gccggtgaat gcggttactg cgcccccttc aatctccagg
      361 atccgatgtg ccagtacatt taccatgcgc cggtcgtgcg tgatcaggat catggcttcc
      421 ggtctgcctg ccagatgggt ctcgagccac tcccgtgcag tcatgtccag gtggttttcc
      481 ggttcatcca gcagcagaag atctgcttca cgcaaaagag ccagcgatag catcagccgt
      541 gcacgccatc cgccgctgag ggctgaaaaa ggcttttcaa agtcttcggg gagaaagccg
      601 agtccaaaga gaatctgacg tgcccgggtg cgaaattcat agccgcccgc cagtgaaaag
      661 gcatgctgtc tttccccgta aagttcgagt aagccgggtt cgtcatgatg cagcctttgt
      721 tccagacggt gcagctcttt ttcttcctga atcaggtctt gaaaaccttc catcgccaca
      781 tcaaacaccg tgcttgccgg atccacttca gggatttggg aaagatagac gatgcgcctg
      841 cgccgcatac gttcaatcgt gccgctgtcc gcttccgact cgccggtgat taagcggaag
      901 agggaggttt tgcctgttcc atttcgcccg ataagagcga tacgttcacc ttcctccacc
      961 cggagattga catcagaaaa gagcggttct ccgccaaaac ttttatttac attttcaaag
     1021 cgaacgagac tcacaaaaac aaaacacttt cttttggaaa agataaacag ttgatgaagg
     1081 aaccagtatt ttcggcatca tgcgcactgc aggcacaggc agggggggct gtaacacacc
     1141 ttttaatttt gtggtatagt atacaaaggt gcgcagggta cagccaaccg gcttgctggc
     1201 tgcgtaattc catggttatg gcaggaaatt cactgccgca gtatgggaga ctgaatttaa
     1261 tgaatatcgc aaaaagcgtt ctgcctgttt tttgtgctct ggtcgtgtcc gctctgtttt
     1321 ctgcctgctc gaccgaagcc atctgggtga gccgtgatca gattgatttt gaccgggacg
     1381 aaagccccat gtattttcac gtggcaaaca ataacgctga aatgggcacc ttcacggtca
     1441 atataacagg caacaaaaac tggatcaaag tatcgccgca gaccattcct tgcaagccgc
     1501 ccacagaatc aggaatggtt atggagcgca tagaagtgcg catagaccga agccgcatta
     1561 catccacagg gaaacatacc ggcgagattc aactgcgtgc gtccggaata aaaacggtca
     1621 cactgaaagt atccgtcgtg caaacttcgg tcaaccccac tttgccgcct ttgagtatca
     1681 ataaccctgt agtgacctat aaatcgccgt cgctcattga gtttgccttc tctttgcgtg
     1741 atcagaaaga ccgtgccgta acgggcgaac cggcgcaatt cggactgcag gcttttgaaa
     1801 gcaggcgccc ggtcggaaca ccggaaggtc tgacgctgcg tcgcggtgcc tcccgtcagt
     1861 tgtggctatc actggttatg gattacagcc agtacatgac ggagataggg gagaacgcca
     1921 ttgacgaaat ggaaagagtc gccacggaag cgctccttcc aagcctgaat gaagatgcgc
     1981 tggtttctgt tcgagcattt tacagaaata cggaaaattc aaaggaaatt gtgccattta
     2041 cagttaaccg tgaatttgcc gctcaggaaa ttcgggagat ccgcagtaaa tatctgcccg
     2101 gattcaattc cggggcgcgg gtttatgaag cgcttctcgc cgctatccag cgctttcctg
     2161 aagaagaaag aacagaaaaa gatgaccgtt acattgtgct tttctgcaac ggaagggata
     2221 ccaccggcgt gccctccatg gaaatcgtca gagaagcggc gctgaagaag aaagtccaga
     2281 tttttgtggc gtgtctgggt gactccatgg atgccgacaa actgattaca ctggcgcggt
     2341 caaccaacgg gcgttttgta gccgctgatt ctttgaatac cctgcagaca gcattccagc
     2401 gcattgtgga agacctttac ggtcagtata ttgtgcgctg ggcgagcgga agagaagata
     2461 ccttcaatat aattccttcc attacgctga cctataatgg cgctgcagct tcctatgaag
     2521 cctcaaaggc ttttgttcct tcccagcatc tgggagaccg tatgcgcgga gagctgattt
     2581 tggtgcaatc ggaaacgccc gggaaaaaca cgacagtctt tttgcgggcg cattatgtgc
     2641 catacggcat ctcagccctg cagctgcgtg tacagagcag ccactcctat gacgtggcct
     2701 tagtggacgc catagatgac ggattgctgg cgggctggca attggaaacc gaagatacgc
     2761 aggcaggcga aaaactgatc agggccacgg gcagtgcttc aattccattt gccgctttcg
     2821 gagccatgct tcgctttgaa tttgatgagg aagtggaaga cgcattcaca tcatttgtaa
     2881 tagaaaatgc aggatatgtc gacggacagc gtttcgtgct gatgtagaag tacgctcagt
     2941 gctcctctat gcagaggcgc tccagaacag caggaacggc gcagcaatca ggcaatccga
     3001 tatcttccac cacgaggctg cccaggtaat tttttgctgc cggattgaga aacccctgtt
     3061 tcgcaaaggc aaaagtcacc gtcagatcag cccgtatgca gataccgccc ggtatacctg
     3121 agtctgcatc cagaccggaa gggacatcga cagcaagggt gtacatttca ggccagtgct
     3181 ggatggcggc aagaacgaga ccctgcgcgc cgccttttgc tccggtgccc agcatggcat
     3241 cgacaatggt tcccttccgt tcaagagact gcagggcgga agcgaccgca tcgggctcgg
     3301 tcagggattc tattgcaccg ccaagcttgc ggtagacttc cataaaaac
//
LOCUS       DAOPWB010000216         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_31259_length_3173_cov_4.845414, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000216 DAOPWB010000000
VERSION     DAOPWB010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31259_length_3173_cov_4.845414"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..306)
                     /locus_tag="PKN92_12900"
     CDS             complement(157..306)
                     /locus_tag="PKN92_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49735.1"
                     /translation="MKHLRLVSRPPATAQMDAGQLITMLAKILQAVGAMMVTKEENTQ
                     GPYDW"
     gene            660..>3173
                     /locus_tag="PKN92_12905"
     CDS             660..>3173
                     /locus_tag="PKN92_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49736.1"
                     /translation="MYRISVALLIGMLFLPLGALGQSDPCTLMDLTVQTPITLQELPS
                     LLVETLALPYGVSDLSSPQMKVNIWLKVLHRNQKANGEKLEIFFNKLPVLTLPADGWP
                     NIWTDEGWHLLPVNVSVGEPIAGAIEIKSYTRTDVPDRKPTTILIGAVQLAGTELTGG
                     PDQADTLKKAVYQSELTQSGLTIPGAYKITADGIAGEAQRYTARLGGIGAPRLSASVR
                     FNAGVGAEGEGEGEGEGEGEGEGEGEGEGEGETGGPVNAFIQLRYGDEPLNGELQVTG
                     FPSPYILGTNAFNVGGACVRVFDYIPDHMILMPFLPKVRVSVDPSFSSAFWMVDDFCL
                     TYLSPALEMADPEWIGTLNLIPNGDFEAGSYPYVVESLSGSITCPTAHDIIKRVSDAC
                     HGNGAAVFRFRIADAQIASCDVERLAGKSTNATLVITGLNLSSITFVALVPETYAGRP
                     FAEGNYIQLSPAGAPSFVGDMEEIRFPFHFGQPFNAASNGNVICTDGLASFEVAINGE
                     LKPGLLDACTTLIDTVPPALVIDTPGGVANACAGSPSICGVNRVFPDANWLMPAGWVP
                     GQIDPDVTPKNAASFAVSDMHVYVNPGSRQDVPYSSADALSYDFLEMLVKARFVDVPP
                     SQGGTSFPVTLSGFSAAAATPATPYVFPEGRPGTAQWNGTGVPATTVDSYTQTLVSPA
                     DMTVAWRVRMQRGSNADDWQTAFKLLASDRAGNQPDTASQNNIVVHWLYRTAARFSDK
                     MQVRSDPPSAAWQLVGPKMPEAAPACRQLARFRIYRVEDNGTLTPLGQSEWLGDPLQG
                     STLLGGTAFQAILRNNIGVPLCLDIIGADGGPVLRKRRPP"
BASE COUNT          699 a          802 c          899 g          773 t
ORIGIN      
        1 cgagtgagtt ttcttccagt gtgtgttgga aaaatagcgc ccctgtgaaa ttaagtagca
       61 ggataatagc attttcaggg atggcactgc aattttattt gcagactacg cccgaaaacg
      121 aggcgagata gagcagaaaa aagccggaaa aaggcgttac cagtcgtagg ggccctgggt
      181 gttttcttcc ttggtgacca tcatggcgcc cacggcctgc aggattttgg caagcatggt
      241 gatgagctgc cctgcgtcca tttgtgctgt ggcggggggg cggctgacca gtctcaggtg
      301 tttcatgggg gtatcctttt caaaatgtat ggctttttga ccgattgaga accgcacaga
      361 agaaaaacgc ctgaatactg ataaaagtat acagggcaag cctcttttcc cttgcataaa
      421 agcattctgt tactttccgg gtgtgccagt ttacacggga tcggcacccg gactgccatg
      481 caagggaaag cggcctgttt ttactgctga aaatgcatta aaaaattgac agaaaatcac
      541 ttctaaggta taatttatgt aataatactc caagtgtgca gagaaaagca cacagccggc
      601 acggttttga gaaaaaaccg gtgaaaagta ctccgggaaa gcactaagag gagaaagtta
      661 tgtaccgaat ttctgtggca ctgctgatcg gcatgctgtt tctgccgtta ggagcactgg
      721 ggcagtccga tccatgcacc ctgatggatc tgacggttca aacgccgatt accctgcagg
      781 agctgccgtc tcttttggtg gagacgctcg ccctgcctta tggtgtgtca gacctgagtt
      841 ctccgcagat gaaagtcaat atctggctga aggtgctgca ccgcaaccag aaggcaaatg
      901 gggaaaaact ggaaatcttc tttaataaat tgcccgtgct gacacttcct gcagatggct
      961 ggccgaacat ttggactgat gaaggatggc accttctgcc tgtcaatgtt tctgttggtg
     1021 agccgattgc cggagcgatc gaaattaaaa gctatacccg tacagatgtg cccgacagaa
     1081 agccgacgac tattcttata ggagcagtgc agctggcagg gacagaatta actgggggtc
     1141 ccgaccaggc tgataccctg aaaaaagccg tttatcagag tgaactcacg cagtccggcc
     1201 tcacgatacc gggtgcctat aaaattactg ccgacggtat cgcaggagaa gcgcagcggt
     1261 atacggctcg tttgggcggt attggggctc cgcgtctgtc ggcttccgtt cgctttaatg
     1321 ccggtgtcgg tgccgaaggc gagggcgaag gcgagggcga aggtgaaggt gaaggagagg
     1381 gagagggcga gggcgagggc gagggcgaaa ccggcgggcc ggtgaatgca tttatccagt
     1441 tgcgttacgg ggatgaaccg ttgaatggcg aactgcaggt caccgggttc ccgtcaccct
     1501 acatcttagg tacgaatgcc ttcaacgtgg gcggggcgtg tgtgcgtgtt tttgactaca
     1561 tccctgacca tatgatattg atgccgtttc ttcccaaagt gcgggtttcc gttgacccct
     1621 ccttctccag tgctttctgg atggtggatg acttttgtct gacttatctg tcgcctgcac
     1681 tggaaatggc tgatccggag tggattggta cgctgaacct gattcctaac ggcgattttg
     1741 aggcgggttc ctacccctat gttgttgaat ccctttcagg aagcatcacc tgtcccacgg
     1801 cacatgacat tattaaaagg gtgagtgatg cctgccatgg caacggagcg gccgtttttc
     1861 gttttcgcat agcggatgct cagatcgcat cctgtgatgt ggaacggctg gcaggaaaaa
     1921 gcaccaacgc cactctggtg atcaccggac tcaatctgtc atccataacc tttgttgctc
     1981 ttgttccaga gacctatgcc gggaggcctt tcgcagaagg caattatata cagttgagcc
     2041 cggcaggagc cccttctttt gtcggtgaca tggaagagat ccgttttcct ttccacttcg
     2101 gccagccttt taatgctgct tccaatggca atgtaatctg caccgacggt ttggcgtcct
     2161 ttgaggtcgc catcaacggt gagttgaagc cgggcttgct cgatgcctgt accacgttga
     2221 tcgataccgt tcccccggca ctggtaattg acacgccggg cggtgtggct aatgcctgtg
     2281 ctggctcgcc ctccatctgt ggcgtgaacc gtgtgtttcc cgatgcgaac tggctgatgc
     2341 ctgcaggatg ggtaccggga cagattgatc ctgacgttac acccaaaaat gcagcctctt
     2401 ttgctgtctc tgatatgcat gtttatgtca acccgggctc cagacaggac gtcccctatt
     2461 cttcggccga tgctctttca tacgactttc tggagatgct ggtaaaggcg cgctttgtcg
     2521 atgtgcctcc ctcacagggc ggaacaagtt ttcctgtaac cctttcagga ttttctgcag
     2581 ccgccgccac accggcaacg ccttatgttt tcccggaagg gcgtcccggt accgcccagt
     2641 ggaatggcac cggagttcct gccactactg tagacagcta cacccagacg cttgtctctc
     2701 ccgcagatat gacggtggcc tggcgtgtga ggatgcagcg gggcagcaac gccgatgact
     2761 ggcagaccgc atttaaactt ctggcgtcag accgtgcagg caaccagcct gacacagcca
     2821 gccagaataa tattgttgtg cattggctct acagaacagc agcccggttt tctgacaaga
     2881 tgcaggtacg ttcggatccg ccttcagcgg cgtggcaact tgtagggccg aaaatgcctg
     2941 aggctgcacc ggcgtgccgc cagctggcac gcttccgcat ctatcgtgtg gaagataatg
     3001 gcactctgac gcctttgggt caatccgagt ggcttggtga cccgctgcag ggttccaccc
     3061 tgctcggagg aaccgctttt caggcaattc ttcggaacaa cataggcgta ccgctgtgtc
     3121 tggatatcat cggggcggat ggcggaccgg ttcttcggaa acgccggcct cct
//
LOCUS       DAOPWB010000217         3155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_31532_length_3155_cov_5.193548, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000217 DAOPWB010000000
VERSION     DAOPWB010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31532_length_3155_cov_5.193548"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..345
                     /locus_tag="PKN92_12910"
     CDS             55..345
                     /locus_tag="PKN92_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family transcriptional regulator"
                     /protein_id="HNZ49737.1"
                     /translation="MPVYTYQVINEDGSEGEIFETIHGMNDPPLTVHPSTGQPVRRIY
                     TAPHIAGWSNSRKAKELTSDANLEKHGFTKYVRNGKGHYEKRTGKGPDHLQP"
     gene            1070..2278
                     /locus_tag="PKN92_12915"
     CDS             1070..2278
                     /locus_tag="PKN92_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNZ49738.1"
                     /translation="MIKRIELRGCEAGKEEAVDVVVRDDGKVESVTPAGRHAPDFGSE
                     AHVLGPTLFDMQVNGALGVDLQDPALTEERFRFLNTVMQCVGVERWMPTLITDSIDAL
                     EEKCHRLAQWMEDPEIASHIPGIHLEGPFISPADGPRGAHPARHVQRPDIKIFDRLQR
                     AAKRKIRMITLAPEQPGALQLIRHLSQSVMLVSLGHHAANEKAIFDAVEAGAAMCTHL
                     GNGMSPMIHRHYNPLWLQMIHDDLAASVIADLEHLPISMLEAILRCKGEGSIVLVSDS
                     VFLAGMKPGKYRLFDADVEMKESGRICLSGTDLLAGSSLFLPQAVAYMATFTSMSLED
                     AFAAASTVPAQLLGIRTRRWRPRTGANAGFALYPPLEYRARFLPIVTVTGGKIYTPSI
                     RDITHDFRNN"
     gene            2302..>3155
                     /locus_tag="PKN92_12920"
     CDS             2302..>3155
                     /locus_tag="PKN92_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNZ49739.1"
                     /translation="MARSDTHLRITISGTGFAGDFTARAFSMIPHKNGVALEFAGVTS
                     GHLGNARLFAQRHGVTAAFESHQEMLDAVQPHIDCIACANHAHIRYLLEAADAAVPVI
                     VLEKPPMIWPGYLENRAADAAMRKAESMDVLHEALEAVEASGSKLLYAENFVYVDGVK
                     ALAQLLREVESLEKGKILLQQGVCAHQGSHAPDYDIPEKSGGGALFNKACHPLGSALY
                     LKQVEGLLREGVPIRPVKVSAVVEAVLRRQPPASGEFFRVMRQVDDYARITVVFEDGT
                     LAELTGHDL"
BASE COUNT          735 a          785 c          905 g          730 t
ORIGIN      
        1 aaacgagccg ctgcgcgcaa cgcctgatcc ggatgctgca ggaaaggaac gatcatgccg
       61 gtctatacct atcaggtgat taatgaagac gggagtgaag gcgagatttt cgaaacaatc
      121 cacggaatga acgacccgcc tcttaccgtg catccgtcca ccgggcagcc tgtccgccgt
      181 atttatacgg caccgcatat tgccgggtgg agcaactccc gtaaagcgaa agagctgacc
      241 agcgatgcga atctggagaa gcatggcttt accaaatatg tgcgtaacgg aaaaggtcat
      301 tatgagaaac gtacgggcaa aggtcctgat caccttcagc cctgatcttt tgatttgtag
      361 tatggaatac aaataaaaat caaaagagcc aatgcgtgaa ataaggaaga acagatgcgg
      421 gctggcacgg aagtgcgctt tagtatccga aactaatttc acatttaata gttgaatcct
      481 taaaaagagg cgcaggcaac tctttatctg ccctgggaca gtatacttca ggtgcgcgcc
      541 ccccctgatt tcagcaaaaa aaatactttg aaaaaagtac aaagaagtct tgaatttgaa
      601 atccctttgg tatatactta agttgttgct gatgggctga aacaaaatga cgtttgaatt
      661 gtaacgttat tgagcagcaa cgcttttaaa agttgaatct tatttactga ggtaaaaaac
      721 ctatgccctc gggcattttt caggggtcga ttttcctctg caacgctctg ttcccctcag
      781 agccaaaaca cctcaccgga gatgccggtc ccccccctcc cggcatctcc ccctttaaaa
      841 attaatcaga aggttttcct ctgcaacgct ctgttcccct cagagccaaa acacctcacc
      901 ggagatgtcg ggccgccccc tcccggcatc tccccctttt tttatcaacc ggaagatact
      961 tccggttttt ttatgcctgc tttcctgaga aaaatatttt aacgtgcagg gaagtggtgt
     1021 tttacacaag ggctggttta ttatgacggg acaccctgga ggcatttgta tgataaaacg
     1081 gattgagttg cgcggttgtg aagccggaaa agaagaagca gtcgatgtgg ttgtgcgcga
     1141 tgacggtaaa gtggagagtg tcactccggc aggcaggcat gcccccgact tcggcagcga
     1201 agcgcatgtg ctggggccga ccctgtttga tatgcaggtg aatggggcgc tgggagttga
     1261 tctgcaggac ccggcactga cggaagaacg ctttcgcttt ctcaatactg tgatgcaatg
     1321 cgtcggtgtg gaacgatgga tgccgacgct gatcaccgat tcgattgatg cgctggaaga
     1381 aaaatgccat cgtctggcgc agtggatgga ggatccggaa atcgcatccc acattcccgg
     1441 cattcatctg gaagggcctt ttatctctcc ggccgacgga ccgcgcgggg cccatcctgc
     1501 ccggcatgtg cagcggcccg atataaagat ttttgaccgg ctgcagcgtg cggcgaaaag
     1561 aaagatacgc atgattacgc tggcacccga gcagccgggg gcactgcagc tgatccgtca
     1621 tctcagccag agtgtgatgc tggtgtcgct gggtcatcat gccgctaatg aaaaggcgat
     1681 ttttgatgct gtcgaagccg gtgcggcaat gtgcacccat ctgggcaacg ggatgtcgcc
     1741 tatgatacac agacattata atcctctctg gctgcagatg attcacgacg atcttgccgc
     1801 atcggtcatt gcggatctgg aacatctgcc gatatctatg ctggaagcaa tactgcgctg
     1861 taaaggcgag gggtcgatcg tgctggtttc ggacagtgtt tttcttgccg ggatgaagcc
     1921 gggcaagtac aggctttttg atgccgatgt tgagatgaaa gaaagcggca ggatctgtct
     1981 gagcggaaca gatctgcttg cagggagcag cctctttctt ccgcaggctg tggcgtatat
     2041 ggcgaccttt accagcatgt cgctggaaga tgcctttgcg gcggcttcca ccgttcccgc
     2101 acagctgctc ggaatcagga caaggaggtg gcgcccccgt acgggtgcca atgcgggttt
     2161 tgccctttat ccgcctctgg aatacagagc caggtttctg cctattgtga cagtgacagg
     2221 cgggaaaata tatactccct cgatcagaga tataacgcat gattttcgta ataactgatt
     2281 gaagaaaggg ttcagaaggc catggcacgg tcggatacac acctgcgcat caccatcagc
     2341 ggtaccggtt ttgcgggaga tttcacggca cgcgcctttt ccatgattcc ccataaaaac
     2401 ggtgtggcgc tggaatttgc cggggtcacg tccgggcatc tgggcaatgc gcgccttttt
     2461 gcacagcgcc acggtgttac tgccgccttt gagagccacc aggaaatgct ggatgccgta
     2521 cagccccata tagactgtat cgcctgcgcc aaccatgcgc atatccgcta tcttctggaa
     2581 gcggcggatg ctgccgtgcc ggtgatcgtg cttgaaaaac cgccgatgat ctggccgggc
     2641 tatctggaaa accgggctgc cgatgcggcg atgcgcaaag cggagagtat ggatgtgctc
     2701 catgaagcgc tggaggcggt ggaggcatcg ggatccaaac tgctttatgc ggagaatttt
     2761 gtgtatgtcg acggggtgaa ggcgctggcg cagctgcttc gcgaggtgga gtcgctggag
     2821 aaagggaaga ttctgctgca gcagggtgtg tgcgcacatc agggttcgca cgcccccgat
     2881 tatgatatac cggaaaagtc cggcggcggt gcgctcttca ataaggcgtg tcatcccctc
     2941 ggttccgctc tttatctgaa gcaggtggaa gggctgctca gggaaggagt gcccatccgc
     3001 ccggtaaaag tgtcggcagt tgtggaggcg gtgcttcgcc gccagcctcc ggcatcgggt
     3061 gagtttttcc gggtcatgcg gcaggtggac gactatgcgc ggataacagt tgtgtttgaa
     3121 gacggcaccc tggcggagct gaccgggcac gatct
//
LOCUS       DAOPWB010000218         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_32643_length_3079_cov_4.707672, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000218 DAOPWB010000000
VERSION     DAOPWB010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32643_length_3079_cov_4.707672"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..956
                     /locus_tag="PKN92_12925"
     CDS             312..956
                     /locus_tag="PKN92_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ49740.1"
                     /translation="MSLLISEIFASIQGESSRAGLPCIFIRLAGCNLKCRWCDTTYAK
                     EGGSEKSINTILEAVGSWKIRLVEITGGEPLYQPECSTLAQQLLQAGYTVLVETNGSQ
                     PIAQLPDGVIRIMDIKCPDSGMADHFYSANVEALTEEDEIKFVLASRRDYEYGVAMIR
                     QYRLDQRCRHLFFSPVLRDLPAAQLAEWMLADLPPARLHLQLHTVIWPGVERGV"
     gene            complement(966..1724)
                     /locus_tag="PKN92_12930"
     CDS             complement(966..1724)
                     /locus_tag="PKN92_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49741.1"
                     /translation="MKCFKCSGFVVLFVLISGIASLLYADEDGALGRQVFEARQTSVV
                     TLHTVVGINFGTGEMENEQEANATMIAPDGLAVLALSAVDPAQMISGMNLGGDEMTSR
                     IISMRMILTDGSEKPAEVVLRDKDLDLAFVRLTEKPESPLPFVDLHDAGEPALLDNVV
                     CLMQYGRVARRSHAAFLDRVELIVTKPRLFYALGDHRSRQVVCSPVFTLENKIVGVGV
                     MRLLRGDGDRSPDDMMVIIVPAAQIQELTAQLPE"
     gene            complement(1907..2755)
                     /gene="prmC"
                     /locus_tag="PKN92_12935"
     CDS             complement(1907..2755)
                     /gene="prmC"
                     /locus_tag="PKN92_12935"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNZ49742.1"
                     /translation="MPLTVAQILQQSAERLSVFSETPRLDAELLLSRALRISRASLLS
                     RMQEVVEDTAFFDTLLARRLAFEPLAYIFGEWEFYGRSFLVRPPLLTPRPETEHLIEV
                     GLAFLSEKEMPCRIIDVGCGTGCIAVTLACEAPRHEVFALDIRPDAAALTKMNARQHR
                     AEISVLRSDLLAAFPGNTPLFDLIVSNPPYVPEDEWSRLSPVITRHEDPGALLAGRDG
                     LDIYRRLIPQAKTLLFPGGALVLETAEDQHPPLKALLSKSGFVEIRSFSDLAGFDRIL
                     SARCPL"
     gene            complement(2757..>3079)
                     /locus_tag="PKN92_12940"
     CDS             complement(2757..>3079)
                     /locus_tag="PKN92_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNZ49743.1"
                     /translation="GMVVTCQDEKSQHKNKAQALRVLRARLLDARMQEEAAARSESRR
                     SQVRSGDRSEKIRTYNFPENRVTDHRVHLSLYRLREVLEGDLDEFFEALAIADRAERL
                     AAQD"
BASE COUNT          773 a          800 c          853 g          653 t
ORIGIN      
        1 ttgccgtcaa ctacgcggga aagtctttga aagtaaggat tattttgtgt ttgtacgtcc
       61 tgaagccttt ggtgtggatc agggcggtgt gtaagtcaag acgtccatat ccttattaag
      121 attcctgcct tcgcaggaat ggcgggaaaa ggagcaggaa cgacggggag aggcagggat
      181 gacagggcgt caagagggct ctgggaggaa tgaacgatgt tgagggacac ggggaggaac
      241 ggcgggaaaa gaaaaataat ttggctgcgg ccgtggtgtg gtgtgtaaaa tacccgaaag
      301 aggagcttct tatgtcttta ctgatttctg aaatttttgc gagcattcag ggtgagtctt
      361 cccgggcggg ccttccctgt atttttatcc gtctggcagg atgcaatctg aagtgcaggt
      421 ggtgcgatac aacctatgcg aaagaaggcg gttcggaaaa aagcatcaac accattttgg
      481 aggctgtcgg ttcctggaaa atcaggctgg tggaaataac aggcggagaa cctttatacc
      541 agcctgaatg cagcacactg gcacagcagc tgcttcaggc gggatatacg gtgctcgttg
      601 aaacaaacgg ttcgcagcca atagcacagc ttccggacgg tgttatacgc attatggaca
      661 tcaagtgtcc ggacagcggt atggcagacc acttttactc agcaaacgtg gaagctttga
      721 ccgaagaaga cgaaataaaa tttgtcctgg caagccgccg tgattatgaa tacggcgtgg
      781 caatgattcg acagtaccgg ctggatcagc gctgccgtca cctctttttt tcgcctgttc
      841 tacgcgatct tcctgccgct cagctggcag aatggatgct tgcggatcta ccgcccgccc
      901 gcctgcatct gcagctgcat acagtaatct ggccgggtgt tgaacggggc gtctgaaagc
      961 cgtttttatt ccggcagctg tgcggtgagt tcctggatct gcgctgcagg aacaataatc
     1021 accatcatgt cgtcgggtga acggtctcca tcaccccgca gaagacgcat gacacccaca
     1081 cctacaatct tattctccaa cgtaaaaaca ggagaacaga cgacctgtct tgaccgatgg
     1141 tcaccgagcg cataaaatag gcggggcttg gttacgatca gttccacccg gtcgagaaag
     1201 gcggcatgcg agcgccttgc taccctgccg tactgcatca gacagacgac attatcgagc
     1261 aaggcaggtt ctccggcatc gtgcaggtca acgaagggga gcgggctttc cggcttttcc
     1321 gtcaggcgga caaaagcgag atcaagatcc ttgtcgcgaa gcacgacctc cgccggtttt
     1381 tcagatccgt ccgtcaaaat catgcgcatg gatatgattc gggaagtcat ttcatcgccg
     1441 cctaaattca ttcctgaaat catctgtgcc ggatcaacgg cggacagagc aagcacagca
     1501 agcccatccg gggcaatcat ggtggcattc gcttcctgct cattttccat ttcgcccgtg
     1561 ccgaaattaa taccgaccac agtatgcagg gtaacaacac tggtttggcg ggcttcgaac
     1621 acctgacgac ccagtgcgcc gtcctcatcc gcataaagga gcgaggcaat ccctgagatc
     1681 agaacaaaca gaacaacaaa accgctgcat ttaaaacact tcatcgcgga aactccttgc
     1741 cgggggcgat tatctgggct gagagcacgg gtggggcgaa caggatgccg tctgacagtt
     1801 gaaacaaatt tatgatacag aaaagtccgc ttgtgattcc attcatgcaa atgatgaagt
     1861 acctcatgtc tttattccct ttcggtactg ccctatgacg ttgaattcac aggggacacc
     1921 gggcagaaag gatacggtca aaaccggcca gatcggaaaa acttctgatt tcgacaaagc
     1981 ccgatttcga cagcagagct ttaagcggcg gatgctgatc ttcggccgtc tccagaacca
     2041 atgcgccgcc ggggaaaagc agcgttttcg cctgtggaat aagacgacgg taaatgtcga
     2101 gaccgtcccg acctgcaaga agcgcgccgg gatcttcatg ccgggtaatc acaggcgaca
     2161 gacgactcca ttcgtcctcg ggcacatagg gcggattgga cacgataaga tcaaacaaag
     2221 gtgtattacc gggaaaagcg gcaagaagat cgctccgcag tacggagatt tcggcacgat
     2281 gctgccgggc attcatttta gtcagcgctg cggcatcggg gcgaatatct agagcaaaga
     2341 cctcatggcg gggtgcctca catgccagcg tcacagcgat gcagccggta ccgcatccta
     2401 catcaattat gcggcaaggc atttctttct ccgacagaaa agccagtccc acctcaatca
     2461 gatgttctgt ttcgggacgg ggcgtaagca gcggaggccg cacaagaaag gagcgtccgt
     2521 aaaactccca ttccccgaaa atatacgcca gaggctcaaa agcaaggcga cgtgccagaa
     2581 gcgtatcaaa aaaagccgtg tcctccacga cttcctgcat tcttgaaagc agcgaggcac
     2641 gggagatccg caatgcccgg gaaaggagca attcggcatc gagccggggt gtctcggaaa
     2701 aaacagacag ccgttcagca ctttgctgaa gaatttgtgc taccgtgaga ggcatctcaa
     2761 tcctgagcgg ccaggcgctc cgccctgtcg gctatcgcca gcgcctcgaa aaattcatcc
     2821 agatcgccct ccagcacttc ccgaagtctg tacagggaaa ggtgaacacg atgatcggta
     2881 acacggttct ccggaaaatt ataggtacgg atcttttcgc tccgatcgcc tgagcgaacc
     2941 tggctgcggc ggctttccga ccgggcggcg gcttcctcct gcatacgggc gtccagcagt
     3001 cgggcacgca gcacacgcaa tgcctgggcc ttgttcttgt gctgggattt ttcgtcctgg
     3061 caggtgacaa ccatacccg
//
LOCUS       DAOPWB010000219         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_33077_length_3051_cov_4.422897, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000219 DAOPWB010000000
VERSION     DAOPWB010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33077_length_3051_cov_4.422897"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1513
                     /locus_tag="PKN92_12945"
     CDS             398..1513
                     /locus_tag="PKN92_12945"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNZ49744.1"
                     /translation="MRLFGKNYSKTALRKRLGNMDQLAGIRLVSFDDGNERPVRAALF
                     HTGSGLEFTVLLDRGMDIASASFQGKAMGWRSTTGNVAPQYFEPEGLRWLRSYFGGLV
                     TTCGLTRVGAPGADSALQGDGLHGRISHIPARDIQVSQAWEGDRYLLSVSGVMRETSV
                     FGENLTLRRTISTELGASSFRIRDVVCNEGFTRTGLSLLYHCNIGWPVLDQGSRMIAP
                     VRALAPRDSAAEKGMETWDGMDAPTHGYREKCYYHDMIPDKKGEVTVALVNDGFPDNG
                     FGVYIRYNKKELPRFVQWKQMGEQVYVCGLEPCNCGIEGREKDEALGLLDMLNAGEER
                     AFNLEFGVLTDGGQVAALQQTMAKNKPQFKKSYTELL"
     gene            complement(1536..2456)
                     /locus_tag="PKN92_12950"
     CDS             complement(1536..2456)
                     /locus_tag="PKN92_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNZ49745.1"
                     /translation="MKKYTSFIILAFAAAFILLPSLFAVADTITLKSSVQIQGKIEKE
                     VGEFMHVRVGDRSIRVRKDEIAEVEKNDLDGSYDLEAAKTAAQEEDRKLTETLGLNEE
                     QRDTVYRYISLLNNPDVSKSRDARAALVKMQSDLNIVPYIVFLLPSYLPPVVLSVLEL
                     ITEISPDQAKEVLREYAFFTDENVRAKALEMLGVIRDKSSAELMMRGLLDHTGIVKIG
                     ACKALAMIRAQEATPLLIHFFDQSDLRIQNNVRQALSALWSDGTTTVSFNSQQEWMAF
                     WQEKSASVQKTVVLDHLEPLVEEGTHFSGC"
     gene            complement(2540..>3051)
                     /locus_tag="PKN92_12955"
     CDS             complement(2540..>3051)
                     /locus_tag="PKN92_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49746.1"
                     /translation="QPNNVYVYTKISEEHAVRYDESGGTVKKVADRLVQLGKNPASVL
                     NRASFQYMNPANNWVSEETLREFWWNDEMSRSRFSPWRFDGVLYSNNAIFAITRSDKR
                     HGSNTEGKMLVRGAMICPDIGVLCAGTDATGNESFRLFYDPRVQEFWAPHDTTQVVFQ
                     RLVYKTESA"
BASE COUNT          718 a          731 c          828 g          774 t
ORIGIN      
        1 ttgtccgtat tgcggggttc cgcgatcggg ctgcttaaaa acagtcggca tgtccggtat
       61 cttaacaggc tcagtcgaag aaggcggcgg cggtggcggc ggcggtggtg gcggcggtgt
      121 agaggttgta aaactgctgg aagggagagc aggctcctga ggtgcctggt ttttgttgat
      181 aaagcctaaa acaaagggga ctgctgctat aatggctaaa acaccgaata tgcctatagt
      241 aactttaggg ttcattcaat cagccttcta cacgtcctgt attttgtaag tatcagtgta
      301 ttctgttaga gtaaaacaat cttatattcg catagcgata tgtttctttc attatagcat
      361 aacctgcagc ctcgtactgt aaaaccggag aagtaccatg cgccttttcg gaaaaaacta
      421 ttcaaagaca gcactgcgga aacgtctggg taatatggat cagcttgccg ggattcgcct
      481 ggtttctttt gatgacggca atgagcggcc ggtacgggcc gctttattcc acacaggcag
      541 cggtcttgag tttactgtgc ttctcgacag aggtatggat atcgcttctg ccagttttca
      601 gggaaaagcg atgggctggc gttcaaccac cggaaatgtg gctccgcaat actttgagcc
      661 tgaaggtctc cgctggctgc gcagctattt cggagggctt gtaacgacgt gcgggttaac
      721 cagagtaggc gcgccgggcg cggacagcgc cttacagggc gacgggctgc acggcaggat
      781 cagtcatatt ccggcgcggg atattcaggt ttctcaagcc tgggaagggg accgctatct
      841 tttatctgta agcggtgtga tgcgtgagac ttctgttttc ggcgaaaact tgaccttgcg
      901 ccgtacgatc agcacggaac tgggggcaag cagtttccgg atccgggacg tggtctgtaa
      961 tgaaggcttc accagaacag gcctctctct gttatatcac tgcaatatcg ggtggccggt
     1021 acttgatcag ggttcccgta tgattgcgcc ggttcgtgct cttgcacccc gtgatagcgc
     1081 tgcggaaaaa gggatggaaa cctgggatgg catggatgca cctacgcacg gctacaggga
     1141 gaaatgttat taccacgaca tgattcccga taaaaaagga gaggttaccg ttgctctggt
     1201 caacgacggt tttcctgaca atggtttcgg ggtttatatc cggtacaaca aaaaagaact
     1261 gccccgtttt gttcaatgga agcagatggg tgagcaggtt tatgtgtgcg gtcttgagcc
     1321 gtgcaactgc ggcattgaag gcagagaaaa agatgaggca ctgggtctgc ttgacatgct
     1381 caatgccggg gaagagcgtg cgtttaacct ggaattcggc gtacttacag acggcggaca
     1441 ggtggctgca ctgcagcaga cgatggcgaa aaacaagcca cagttcaaaa aaagctatac
     1501 ggagctgctg taatttctac ataaaagaga cggtatcagc atccggagaa atgggtgcct
     1561 tcttccacca gtggctccag gtgatccagt acgacagttt tctgcaccga tgcgcttttt
     1621 tcctgccaga acgccatcca ctcctgctgc gaattgaaag aaacggttgt cgtgccatcg
     1681 ctccacaagg cacttaaagc ctggcgcaca ttattctgta tccgcaggtc ggactgatcg
     1741 aaaaagtgaa tcagaagagg tgttgcttcc tgtgccctga tcatggcaag ggctttacag
     1801 gcaccgattt tgacgatacc ggtgtgatcc agcagcccgc gcatcatcag ttctgcagac
     1861 gatttatccc tgataacccc cagcatttcc agcgcttttg cccgtacatt ttcgtccgta
     1921 aaaaaagcat attcccgcaa tacttctttt gcctgatcag ggcttatctc tgtgataagt
     1981 tcaagaacag acagcactac aggcggcaga taagagggca gaagaaaaac aatgtatgga
     2041 acaatattca ggtcactctg cattttgacc agggcagcac gggcatctct gctctttgag
     2101 acatcgggat tgttcaacag tgaaatgtag cggtagacgg tgtcgcgctg ttcttcattc
     2161 aatcccagcg tctcggtaag tttacgatcc tcttcctgcg ccgctgtctt ggcggcttca
     2221 agatcataac tgccgtccag atcatttttt tcaacctccg caatttcgtc cttccgcacg
     2281 cggatacttc ggtctcccac ccgcacatgc ataaactcgc ccacctcttt ttctatcttg
     2341 ccctggattt gcacgctgct tttaagggtg atggtatcag ctacggcaaa aagagaaggc
     2401 agcagaatga aggcggccgc gaaagccaaa atgataaacg aagtatattt tttcatggaa
     2461 agcctgatct cctttcagca tttgcaacaa agccgggcac agaacaccct gtggattttt
     2521 ctgatatggc gcagtacttt tatgcggatt cggttttgta gacaagtctc tgaaacacta
     2581 cttgtgttgt gtcgtggggc gcccagaatt cctgtacccg cgggtcatag aagagccgga
     2641 aactttcatt tcctgttgcg tcggtaccgg cacacagaac gccgatatcg gggcagatca
     2701 tggctccccg taccagcatt ttgccttctg tattggatcc gtggcgctta tcgcttcggg
     2761 taatggcgaa gatggcattg ttggagtaca gcacgccgtc aaagcgccag gggctgaagc
     2821 gtgatctgga catttcatcg ttccaccaga attcgcggag ggtttcctcc gatacccaat
     2881 tgttggcggg gttcatatac tggaaggaag cgcgattcag cacggaggcg ggatttttcc
     2941 cgagctgtac gagccggtca gcgaccttct tcaccgtgcc gccggattcg tcgtagcgta
     3001 cggcatgttc ttcactaatt ttggtgtata catagacatt gttaggctgg g
//
LOCUS       DAOPWB010000220         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_33368_length_3033_cov_3.912021, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000220 DAOPWB010000000
VERSION     DAOPWB010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33368_length_3033_cov_3.912021"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PKN92_12960"
     CDS             <1..1070
                     /locus_tag="PKN92_12960"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNZ49747.1"
                     /translation="REAVSGWQEKPAVPAEWKPVQVLGQMGIPPWGMIKPVGKRSGLY
                     PPSAALEALLCDLGIPPDFEAPAALRFIHKRTAEGALYFVSNGSASALDAEAVFRSAG
                     GLPILLHPEDGSVRALPEYARLEDGRMKIPLHFEAGESYFVLFSPEMPKSSIPASQKN
                     FVAYEKRAEIPGPWQVSFDPALGGPKEPVLFSELTDWSLHTSPEIRYYSGPAIYARDF
                     EIGEKLAELPGLQIDLGTVNVAASVKLNGRDLGICWKKPYRLPPGAALHAGTNHLEIE
                     VVNLWVNRMIGDERLPADSDRKPDGTLKFWPQWLLDGKSSPSGRISFATWRHWHADDP
                     LQPSGLLGPVTLLAPNSRVED"
     gene            complement(1271..2386)
                     /locus_tag="PKN92_12965"
     CDS             complement(1271..2386)
                     /locus_tag="PKN92_12965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNZ49748.1"
                     /translation="MGAIRILSGIAHTMMHPHHPGQLVLFVTDRCNCRCPMCFNADNV
                     FSGCRAPDLPLENILAIARSLKPLPQLLLSGGEPFLRKDIPEIIRAFHRLSHTSQVSI
                     PTNATQTNRITAACEQILQECPKILLNINLSLDGIGETHDAHRQYPGCYEKLCATYEK
                     LAALRERYQQLTVNIITVISERNVKDAASIIAEIREKFSPNYHALGLLRNDTMERESS
                     HILDAMTDSINVVNSEKESFTRLPAFSHLAPAVAAVVKRKALQSRKTKTRCFKCLAGS
                     RIIVITNDGRLMPCEPLWLEDDVRNSADQNAYCMAFLEEFQYNVKEALKSDSAAKVRD
                     FVKKRLCWCTYACAIQNGILYSPTQYILLIQEMMRKR"
     gene            complement(2373..>3033)
                     /locus_tag="PKN92_12970"
     CDS             complement(2373..>3033)
                     /locus_tag="PKN92_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49749.1"
                     /translation="SPVPLNPSFASRYFALQEYLRYSELIDSGMTDWSFITTRFGLLR
                     REVFEETGGFNESFSMASYEDLEFSSRMDNRHRIALNKSFVVRHHWPDSVWKILKRLH
                     INARGVMQFPASMRKKAGEPFARDRNARLYLGLFGILFLGGLIHPVLWIGAVVAHLAA
                     LWQAKWLIAGCIKHEGVLFTLKAWAVYNLTLLPFATGVAAGLFSMLSQKISGFLYHGR
                     H"
BASE COUNT          724 a          752 c          848 g          709 t
ORIGIN      
        1 gcagagaagc agtatccgga tggcaggaaa agcctgcggt tcctgcagag tggaagcctg
       61 tgcaggtgct ggggcagatg ggcataccgc cttggggcat gataaaacct gtcgggaaac
      121 gctccggtct ttatccgccc tctgccgcat tggaagcgct tctgtgcgat ttgggtattc
      181 cgcccgactt tgaagcgccg gcggctctcc gcttcattca taaaaggaca gcggaaggcg
      241 cattgtattt tgtcagtaat ggcagcgcct ccgctctgga cgcagaggca gtgttccgct
      301 ccgccggtgg cttgccgatt ctgcttcatc ctgaagacgg cagtgttcgt gccctgccgg
      361 aatatgcaag gcttgaagac gggcgcatga agatccccct tcattttgaa gcgggagaaa
      421 gttattttgt tctcttcagc ccggagatgc cgaaatccag cattccggca tcccaaaaga
      481 attttgtcgc ctatgaaaaa cgggcggaga ttccagggcc ctggcaggtc agttttgatc
      541 ccgctttggg cgggccgaaa gagccggttc tcttcagcga gttgacggac tggtctctgc
      601 atacttcacc cgaaatcaga tattattcag gtcccgcgat ttatgcacgt gacttcgaga
      661 taggggagaa gctggctgaa ttgccggggc ttcagattga tcttggtaca gtcaacgttg
      721 cggcatcggt aaaactgaat ggccgggatc tgggtatctg ctggaagaag ccgtaccgat
      781 tgcctccggg tgccgctttg catgcaggca caaaccatct ggaaatcgag gtggtgaatc
      841 tgtgggtcaa ccgaatgatc ggcgatgaac ggctgcctgc agacagcgac cgtaaaccgg
      901 acggtacgct gaaattctgg ccgcaatggc tgctggatgg aaagagcagc ccctccggca
      961 ggattagctt cgccacttgg cggcattggc acgcggacga tcccttgcag ccttcgggcc
     1021 tgctgggacc tgtgacactg ctggctccga atagtcgtgt ggaagattaa caacaatcaa
     1081 aggtagcccc atgcttttat cggatgttgt accccataaa cttgaaaagg gcttttcctg
     1141 cggattgttc cacatcacaa acctcggtta cacgagcgcc catgctatcc ggccaggaga
     1201 cacaatcatg aaacaccgaa actctcataa cgactggacc aaagaatggg ggcttgacat
     1261 ccccgttccc tcatcttttt cgcatcattt cctgaatcag cagaatatat tgggtcgggc
     1321 tgtagaggat gccgttttga atggcgcagg cataggtgca ccagcaaaga cgttttttca
     1381 caaaatcgcg cacttttgcg gcggaatcgc ttttaagtgc ctctttaacg ttgtattgga
     1441 attcttcaag aaaagccatg caataggcat tctggtcagc actgttccgg acatcatctt
     1501 cgagccacag cggttcgcag ggcataaggc ggccgtcatt ggtgataacg atgatgcgtg
     1561 atcctgccag acacttaaag caccgtgtct tcgttttccg gctctgaagc gcttttcttt
     1621 ttacaacggc agcaacggca ggtgcgaggt gactgaaagc cggcagccgg gtgaaagatt
     1681 ctttttcgct gtttacgacg ttgatggagt cggtcatcgc atccagaata tggctggatt
     1741 ctctttccat ggtgtcgttg cgaagcagtc ccagcgcatg atagttgggc gaaaattttt
     1801 cccggatctc agcaataatg gaagcggcat ccttcacatt ccgctcggag atgactgtga
     1861 tgatattgac agtcaattgc tgatagcgtt cccgcagggc agccagtttt tcataggtag
     1921 cacaaagttt ttcatagcaa cccggatact ggcggtgggc atcatgtgtt tcgcctatgc
     1981 catccagaga cagattgatg ttcaataaga ttttggggca ttcctgcaga atttgctcgc
     2041 aagctgccgt tatgcggttc gtctgtgtgg cgttggtggg aatgctgact tgcgaggtgt
     2101 gcgacaggcg atgaaaggcg cggatgattt ccgggatgtc ttttctgagg aaaggctctc
     2161 cgcccgagag cagcagctgt ggcagaggtt tcaatgaccg tgcaatggca aggatgtttt
     2221 caagaggcaa gtccggagcc cggcacccgg aaaatacgtt atctgcgtta aagcacatgg
     2281 ggcagcggca gttgcaccgg tcggtcacaa atagcactaa ctgcccggga tggtgcgggt
     2341 gcatcatcgt atgcgcgatt cctgaaagaa ttctaatggc gcccatgata taaaaatcct
     2401 gatatttttt ggctcagcat agaaaacagg ccggcagcga cccccgtggc aaaaggcaga
     2461 agtgtcaggt tgtagaccgc ccaggccttt agcgtgaata gcacgccttc gtgcttgata
     2521 caccccgcaa taagccattt tgcctgccaa agcgcagcca ggtgtgccac aacggcgccg
     2581 atccatagca ccggatggat cagaccgccc agaaataaga taccgaaaag acccagataa
     2641 agacgcgcat tgcgatcccg ggcaaaaggc tcaccggctt ttttccgcat ggacgcagga
     2701 aattgcataa caccccgagc attgatatgc aggcgtttca gaattttcca caccgaatca
     2761 ggccagtgat gtctgactac aaaggattta ttcagtgcaa tacgatggcg gttgtccatt
     2821 cgcgaactga attccagatc ttcataggaa gccatggaaa aggactcatt gaagccgccg
     2881 gtttcttcga agacttcgcg acgcagtaaa ccgaagcggg tagtgatgaa agaccagtct
     2941 gtcattccgc tgtcgatcaa ttcagagtac cgcagatact cctggagggc aaaataacgg
     3001 gatgcgaagg aagggttcag cggaacagga gag
//
LOCUS       DAOPWB010000221         3023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_33556_length_3023_cov_4.477763, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000221 DAOPWB010000000
VERSION     DAOPWB010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33556_length_3023_cov_4.477763"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PKN92_12975"
     CDS             <1..790
                     /locus_tag="PKN92_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNZ49750.1"
                     /translation="DFEITEQMLRYFIQKVHNRKRLPAWPRVAVSVPSGITAVERRAV
                     LESALQAGATQVFIIEEPMAAAIGAGLPVHEPQGCMIVDVGGGTTEVAVIALGGIVFP
                     RSVRVGGDELDQSIIQHLKRTYNLMVGERTAEEIKIAIGSAYELKEEMEMHVKGRDQV
                     LGLPKTISITSEEIREALREPVNAIVESVRVTLERTPPELSADIVDRGVVLAGGGALL
                     RGLDQLIARETGLHVTIAEDPLGAVVLGGGKFLEELKYFKPEVI"
     gene            849..1793
                     /gene="mreC"
                     /locus_tag="PKN92_12980"
     CDS             849..1793
                     /gene="mreC"
                     /locus_tag="PKN92_12980"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNZ49751.1"
                     /translation="MNPGRLLAEYRPQLILVVLVTLSLFSLITGADSSFIHMGLSRFV
                     NLSAYPFLKLRYATVTGVESTYKVFADPKSIQKENEALQLEVASLRARTARIKELDRE
                     NQRLRRLLAFQQQEPGLSLQPAAVLENYKGMLRIEGGSMQGFQVSMGVITAEGIVGII
                     TEVADFTSIVATLHHVDCRVGAMVLRNRLRAYDGVVHASGSDFNQLCSMEYIDMKEEV
                     RIGDQVVSSPESLFPAGLPVGKIVSLRGGGGLWKSASIEPVVNPYQLDEVLVILRALE
                     ASEYIAGPPASLSGTDVPFISKAPEMPDQRSLQERYAP"
     gene            1790..2323
                     /gene="mreD"
                     /locus_tag="PKN92_12985"
     CDS             1790..2323
                     /gene="mreD"
                     /locus_tag="PKN92_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNZ49752.1"
                     /translation="MIRQRVYKSRVFFLWCFCTLLAAMIQTNWPNLLKLQEVSPDIIL
                     VVLVYFAIAYGEEWAMFTGLLGGLYLDVAVGTTLGHYVLCYVVMGYILGRLSTRLITE
                     HAAIKAGAVFAAGTGWGILYASIQYMQRPQSGMIQPLITNFIPTAFYSAIITPLVFLT
                     LDRIFHRRDYMGGGLQS"
     gene            2320..>3023
                     /locus_tag="PKN92_12990"
     CDS             2320..>3023
                     /locus_tag="PKN92_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49753.1"
                     /translation="MTDNREQERYPGIERRVQQIGFAIAAALLLLGVQLWRLQVLSLN
                     EFSKLAESNRIWQKRLAADRGIIYARDGEVMADNRARVDVIIIPGECPADALDTVCER
                     LETWLNVDRQELDKKITASAKLPFSQILVKRDISRVDQIRVEENQHLLPGVTTIVHPQ
                     RRYLLGETAGQIMGYLGEINQAELDQRQELGYFQGDLIGKGGIELFYESLLHGQDGYM
                     LVTKYASGQPQLRTDKA"
BASE COUNT          687 a          711 c          865 g          760 t
ORIGIN      
        1 cgattttgaa ataacagagc aaatgctccg ttattttatc cagaaagttc acaaccggaa
       61 acggcttcct gcctggcccc gtgtcgctgt cagtgtgcct tccgggatta cggctgtaga
      121 gcgcagagct gtgctggaaa gtgccctgca ggcgggcgcc acgcaagtct ttattattga
      181 ggagcccatg gcagccgcca tcggcgccgg attgccggtt cacgaacctc agggctgcat
      241 gattgttgat gtgggcggcg gtaccacgga ggtggcggtg attgccctgg gtggcatcgt
      301 atttccccgc tctgtccgtg tcggaggtga tgagctggat cagtccatta ttcagcacct
      361 gaaacgcact tacaatctga tggttgggga gcgtacggct gaagaaatta aaatagccat
      421 cggttcggcc tatgagctga aagaagagat ggaaatgcat gtgaaggggc gcgatcaggt
      481 gcttggactt ccgaaaacta taagcattac ctctgaagaa atcagagaag ccctcagaga
      541 gccggtgaat gcaattgtgg aaagcgtccg tgtaaccctt gaacgaactc ctcccgagct
      601 ttccgcagat atagtggacc gcggtgttgt gcttgcaggc ggtggcgccc tgctccgggg
      661 actggatcag ctgattgccc gggaaacggg ccttcatgtt accattgctg aagatcccct
      721 gggtgctgtt gtgctgggcg gcggaaagtt tctggaagaa ctcaagtatt ttaaaccaga
      781 agtaatttaa aggtgcaaat acgatctctg gcgcatgaag aggctctcct tcgggaaggc
      841 aacggcttat gaatccggga agacttctgg cggaatatcg tccccagctc atcttagttg
      901 tccttgtgac actgtctttg ttttcgctca ttacgggggc ggacagctct tttattcaca
      961 tggggctttc ccgttttgtt aatctcagcg cctatccctt tctcaaactg cgttatgcaa
     1021 cggtgacagg tgtcgaaagt acctacaaag tctttgccga tccaaagagc attcagaaag
     1081 aaaatgaggc gcttcaactg gaggtggctt ctctgcgggc caggacggcg cgtatcaagg
     1141 agcttgaccg tgaaaaccag cgcctgagac ggctgcttgc ttttcagcag caggagccgg
     1201 gcctgtcgct tcagccggcc gctgtattgg aaaactataa aggcatgctt cgcattgaag
     1261 gggggagtat gcagggtttt caggtgtcaa tgggggtaat tactgcggag ggaatcgttg
     1321 ggattattac agaagtggct gatttcacct ctattgttgc cacactgcat catgtggact
     1381 gccgggtcgg tgctatggtg ctccggaatc gattgcgtgc ctatgatggg gtcgtccatg
     1441 cgagcggcag cgattttaat cagttgtgct ccatggaata tatagatatg aaagaagaag
     1501 tccggatcgg tgatcaggtt gtatccagcc cggagagcct gtttccagcc gggttgcctg
     1561 tagggaaaat agtcagcctt cgcgggggcg gcggattatg gaaaagcgca tccatagagc
     1621 cggtagtcaa tccctatcag ctggatgaag tactggtgat tctcagagca ttggaagcgt
     1681 ccgaatacat cgccggaccg cctgcctctc tttccgggac ggatgttccg tttatttcaa
     1741 aagcccccga aatgcctgat cagcggagcc ttcaggagag atatgcccca tgataaggca
     1801 gcgtgtttat aaaagtcgtg tctttttttt gtggtgtttc tgcacactcc ttgccgccat
     1861 gatccagacc aactggccca atctgttaaa gctgcaggaa gtgtctccgg atattattct
     1921 tgttgtgctg gtctattttg ccatcgcata cggtgaagag tgggcaatgt ttaccgggct
     1981 cctgggcggg ctttatcttg atgtcgccgt aggtacaacc ctgggtcatt acgtcttgtg
     2041 ctatgtagtc atgggctata tactgggcag actgtccacc cggctgatta cagagcatgc
     2101 ggcgatcaag gcgggcgccg tcttcgctgc agggacgggt tggggaattc tctacgcctc
     2161 cattcaatat atgcagcgac cacaaagtgg catgatccaa ccgctcatta ccaattttat
     2221 tcctactgct ttttattccg ccatcatcac ccctcttgtt tttctcacac tggatcggat
     2281 cttccacaga cgtgactaca tgggaggggg acttcagtca tgacggacaa tcgcgagcag
     2341 gaacgctacc cgggaatcga acgtcgggtt cagcagattg ggtttgcgat tgcggccgct
     2401 cttttattat tgggcgttca attgtggcgc ctgcaggtgc tctctttaaa tgagttttca
     2461 aaactggcgg aaagcaaccg aatctggcaa aagcgtcttg ccgccgatag aggaattatc
     2521 tatgcccggg atggagaggt gatggcagat aatcgtgccc gggttgatgt gattattatt
     2581 ccgggcgaat gccctgctga tgctctggat acagtatgtg aacggcttga aacctggttg
     2641 aatgttgacc ggcaggagct ggataaaaag ataacggcat cggcaaaact tcccttctct
     2701 caaattctgg taaaaagaga catctcccgg gttgaccaga tccgcgttga agagaatcag
     2761 catctgctgc caggtgtcac tacaattgtg catcctcagc ggcgttatct gcttggggaa
     2821 acagccggcc aaattatggg gtatcttggg gagattaacc aggcggaact ggaccagcgc
     2881 caggagctag ggtattttca gggagatctg attgggaaag gcggaatcga acttttctac
     2941 gaatcactgc tgcatgggca ggatgggtac atgcttgtca caaaatacgc ctcgggacag
     3001 cctcagctgc ggacagacaa agc
//
LOCUS       DAOPWB010000222         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_33856_length_3003_cov_4.716418, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000222 DAOPWB010000000
VERSION     DAOPWB010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33856_length_3003_cov_4.716418"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKN92_12995"
     CDS             complement(<1..582)
                     /locus_tag="PKN92_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49754.1"
                     /translation="MSQSKTRIVPLLLLLVVLALIGGSFYGYLAWEKAKFEGSLPAEK
                     KVAAPTTPQPAERPKPSAVRSFGELSEPPLTLSALRFLPAQADIALGIPPIASLLANG
                     VPLAQDILKDRLDLQAEIQSIAQGIALEMGLSEDKDMAAVLTDLGIDIQSGAAVFLTA
                     KDMVTGALAAETPPKLSKLFDMPGFSLTIALRVL"
     gene            complement(857..1837)
                     /locus_tag="PKN92_13000"
     CDS             complement(857..1837)
                     /locus_tag="PKN92_13000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNZ49755.1"
                     /translation="MNIFSPEIFGLALDNTVDSVVITDLNNVIQYANPAFTKITGFPR
                     DKALGKTPSILRSRHTTLETYQKMWATILAGGWWRGHIINVKANGEEWHSFLSISQVR
                     NPSGRAVGYVGIARDITEMKRLETQLHDMSLEAIYMLAQAAEAKDEVTGNHILRVRAY
                     SEAVAAEMGLTEEEVNKIGYSSILHDVGKLHVPDAILKKAGKLTEDEWEEMKKHPEGG
                     AAILRNKDFYHVARDIAANHHERWDGTGYPAGIKGEEIPLASRIVTAADVFDALTTER
                     PYKKAWTETEAMDKILQDRGKAFDPAVVDAFEALFRRGKISELMRRYADQ"
     gene            complement(1953..>3003)
                     /locus_tag="PKN92_13005"
     CDS             complement(1953..>3003)
                     /locus_tag="PKN92_13005"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNZ49756.1"
                     /translation="DWDLLRQRLNNQRLLGMDYYSGGHLTHGYRRNLSAKMFDAYSYA
                     VDRESGLLDYDAIEAQAMEVKPLVLLAGFSAYPRRINFRRMRAIADKVGAVFMVDMAH
                     FAGLVAGGVFTGDENPIPHAHIVTSTTHKTLRGPRGGFVLCTKEFEEAVDKGCPLVLG
                     GPLPHVMAAKAVAFREANTPAFKDYAHKVVENCRTLAEALKAKGCTLATGGTDNHLLL
                     VNATASYGITGTQAASALRDCGITLNFNSLPFDPNGPLITSGLRIGTAATTTLGMGEE
                     EMKEIAAIIHLALSNTKAALLTTGDNAGKPSKRKYTLDQSSHNEAQARVHDLLSRFPL
                     YPMLDLELLQESFQK"
BASE COUNT          663 a          839 c          794 g          707 t
ORIGIN      
        1 gagcacacgc agcgcaatgg tcagcgaaaa accgggcata tcaaaaagtt ttgaaagttt
       61 cggaggcgtt tctgccgcca gagcacctgt caccatgtct tttgctgtca gaaatacagc
      121 ggcaccggac tgaatatcaa tgccgagatc cgtcagtacc gccgccatat ccttgtcctc
      181 agacagaccc atttctaggg cgattccctg ggcgatggac tgaatctccg cctggagatc
      241 cagacggtct ttcagaatgt cctgtgccag aggaacgcca ttagccaaca gggaggcaat
      301 gggcgggata ccgagagcga tatcggcctg agcgggaagg aaacgaagcg ctgacaaagt
      361 cagaggcggt tcgctcagtt caccgaaact gcgcacagcg ctcggtttcg ggcgttcagc
      421 aggctgcggt gtcgtcggag ccgccacttt tttctcggca ggcagagaac cttcaaattt
      481 ggctttttcc caggccagat acccgtagaa ggatccgccg atcaatgcca gcacaacaag
      541 caaaagcagc agaggaacaa tacgcgtttt tgactgagac ataaaactat cccttccgga
      601 tcgggttgag gttgaacatg atcctgaata atgaccttta tggcacaaac agtgctgaaa
      661 ataaaggaac cgtacgtaat gagtatgaag gatgcatgcc ggaagaatca actttacagc
      721 catttcaagg agcggatttt accgccttga aggtctttga actgcctgaa gcagtgatcc
      781 ggctctactg agaaaaacta tttcggttat gcatgacata gagaagccgg ctcagggctg
      841 acgccgcaga agcaagtcat tgatcggcgt agcgccgcat cagttcgctg atttttccgc
      901 gccggaaaag agcctcgaaa gcgtccacca ctgccgggtc aaaggccttg ccgcgatcct
      961 gcagaatttt atccatcgcc tccgtttccg tccaggcttt tttataggga cgttccgtgg
     1021 tcaatgcgtc aaaaacatcg gcagctgtca cgatacgcga ggccaggggg atttcctcgc
     1081 ctttgattcc tgccggatag cctgtgccgt cccagcgttc atgatggttg gcggcgatat
     1141 ctctcgcgac atggtaaaag tctttgttgc ggagaatagc ggcgccccct tccggatgct
     1201 ttttcatttc ttcccactcg tcttccgtca gtttccctgc ttttttcaga atcgcgtccg
     1261 gcacatgcag tttacccaca tcatggagga tactggagta gccgatctta ttcacttcct
     1321 cttccgtcaa tcccatttcc gctgccacgg cttctgaata ggcacggaca cgcaggatat
     1381 gattgcccgt cacttcgtct ttggcttccg ccgcctgtgc cagcatgtag atggcttcca
     1441 gactcatgtc atggagctgc gtttccagcc gtttcatttc cgtgatgtcc cgggcgatgc
     1501 ccacataccc caccgccctt cctgaaggat tgcgcacctg tgaaatggaa agaaaagaat
     1561 gccattcttc gccattggct tttacattga tgatatgccc gcgccaccag ccgccggcaa
     1621 ggatggtcgc ccacattttc tgataggttt ccagagtggt atggcgtgac cgcagaatgc
     1681 tcggtgtctt tccaagagcc ttatcccgcg ggaaccctgt gattttcgta aaagcggggt
     1741 tcgcatactg gatgacattg ttcaaatcgg tgatgaccac cgagtccacc gtattatcca
     1801 gagcaagccc gaaaatttcc ggtgaaaaaa tgttcataga tcccgacact ccttttcctg
     1861 cggatgacac actccgatga cacactgctt ctgccggagc agccgtatct gcgccgggtc
     1921 ttctgcctca acccgaactg tgcattacct gtttatttct gaaaggattc ctgaagcagc
     1981 tccagatcca gcatgggata gagcgggaag cgggaaagca ggtcatgtac ccgcgcctgt
     2041 gcctcgttat ggctgctctg atccagcgta tatttccgtt tgctgggctt gcctgcatta
     2101 tcgccggtgg tcagcaatgc cgctttcgta ttggacagag caaggtggat aatagccgca
     2161 atttctttca tttcctcttc gcccataccc agtgtagtgg tggcggcggt accgatgcgc
     2221 agtccgctgg taatcagcgg cccgttggga tcgaaaggca gggaattgaa attcagcgtg
     2281 atgccgcagt cccgcagcgc cgaagccgcc tgcgtgcccg tgatgccgta gcttgctgtt
     2341 gcattcacca gcagcagatg gttgtccgta ccgcctgtgg caagggtgca gcctttggct
     2401 ttcagtgctt ccgccagtgt acggcagttc tcaaccactt tatgggcata gtctttgaat
     2461 gcgggggtgt tggcttcacg gaatgccacc gccttggctg ccatgacatg ggggagcggg
     2521 ccgccgagca ccagcgggca gcccttgtcc actgcttctt caaattcttt cgtgcacaat
     2581 acaaaaccgc cccggggtcc gcgcagcgtt ttatgggtgg tggacgtaac gatatgggca
     2641 tgggggatcg ggttctcatc gcctgtaaac acaccaccgg caacgagccc ggcaaaatgt
     2701 gccatatcta ccataaatac ggcgcccacc ttgtcggcaa tggcgcgcat ccgcctgaaa
     2761 ttgatccgcc gcggataggc gctgaatccg gcaagaagta cgaggggctt cacttccatc
     2821 gcctgcgcct caatggcatc ataatccagc agccctgatt cacggtctac ggcataactg
     2881 taggcatcga acatcttcgc tgatagattc cgccggtatc cgtgggtcag atgcccgccc
     2941 gaataataat ccatgcccag aagccgctgg ttattcagcc gctgccgcag cagatcccag
     3001 tcg
//
LOCUS       DAOPWB010000223         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_34633_length_2958_cov_3.054771, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000223 DAOPWB010000000
VERSION     DAOPWB010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34633_length_2958_cov_3.054771"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKN92_13010"
     CDS             complement(<1..251)
                     /locus_tag="PKN92_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HNZ49757.1"
                     /translation="MKKTAIWCLTLLLGAGMLLSLSGCETYGGAAGLGAGVGALAGGV
                     IGHQSGHTLEGAAIGAALGAATGLIAHDIKVKKMRSREET"
     gene            589..1188
                     /locus_tag="PKN92_13015"
     CDS             589..1188
                     /locus_tag="PKN92_13015"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNZ49758.1"
                     /translation="METDRADNYASLDDKALGLAFRNGDAQAFEEIVRRYQGRLYGAA
                     YRITGNREDALDVVQEALMKVYTKIAAWKPTGGFLAWMTRLTTNQAIDLVRSRKRRHH
                     ESLDEGFLSGTAATPLIAPAVDSQSLAQLHELEAHIRKGLVHLSPMQRKVFVARHFEE
                     SSLAEIAEDLNCSVGCVKVHLFRAVQKMRTHLKPLEPDR"
     gene            1241..2284
                     /locus_tag="PKN92_13020"
     CDS             1241..2284
                     /locus_tag="PKN92_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNZ49759.1"
                     /translation="MKLFFFDPVSILSLRRARRAAGPLMAQALYEPLSQAEEHKLQLL
                     VERHPELAQEFESLKAFLPRITLQEDDWCGDLMPALRAQIEEQALLRRGVFSRRGINY
                     VFVASALLLGCGLYAFFMLAGDNRTGTVTPSPLRTAQSASALQNELLAVHDFMNQGRY
                     QEAAARLAQEVKLHPDDPAHADALLQLADIQYTHLQQYDKAYESYKELERSHRPVFNS
                     HWDSNRQLRLLSAALPLNFQPLASLEAAARAESPLHAYEHILTEYPEEIWGEEALRAM
                     TLLVSDGSGEAPDQASALQRLSGMCTEPVALDRICLEIGHCYSDRLANREQAESRHLA
                     LADCAREALAHLE"
     gene            2438..>2958
                     /locus_tag="PKN92_13025"
     CDS             2438..>2958
                     /locus_tag="PKN92_13025"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49760.1"
                     /translation="MSEPIAFEKLLVHRGILDESRLERAKGEAARNGMSLLAVIPQLG
                     YATQESVYEALAEFCEMPFVMLSRRGIEKEIAQKTPARFATHYGFVPVEERGTALVVA
                     ISDPLSPQLLDDIRMVLKRRIEPVVTTPAEITRAIKELYGVGADTMEKMLIEAEGEGA
                     VLTLEGSSVESDLG"
BASE COUNT          616 a          797 c          858 g          687 t
ORIGIN      
        1 gtctcttctc tgctccgcat ttttttgact ttgatgtcat gggcaataag accggttgcg
       61 gcaccgagag cggcaccgat ggcggcacct tccagagtat gtccgctctg atgaccgatg
      121 acacctcctg ccagagcgcc aacgccggct ccgagtcctg ccgctcctcc atacgtttcg
      181 catccgctca gggaaagaag catcccggct cccagcagca gagtaagaca ccagatagca
      241 gtttttttca tatccaactt ccttttttgt tgcggagcag caccgcccac gatcttttca
      301 tctaccaatt cagacgcatt tccccgttga ttattcatta tacacgcacc cgccctccgg
      361 agtcgatgct tcggtttcgt ctgcgttctt ttccgtaaac atgcggaaag gcaaatacat
      421 ttcatttttt atcgggattt gtcgggcata tggaaaagaa atgaaacaaa agtccctctc
      481 cgggaatata ctgtaggaga gaaggatttg tttttgatgg ttctgcatgc cgttcaccgc
      541 ataaaaccgc atttgtaaac cttttgcaag aagagggcgt tcctcccaat ggaaaccgat
      601 cgcgccgaca actacgcatc actggacgac aaagcgcttg gtctcgcttt tcgtaacggc
      661 gatgcgcagg cctttgagga gattgtccgg cgttatcagg gccgcctcta tggtgccgcc
      721 taccgcatca cgggaaaccg cgaagatgcg ctggatgtgg ttcaggaggc actgatgaaa
      781 gtctatacaa aaatagcggc gtggaaaccg acaggaggct ttcttgcctg gatgacccgg
      841 ctgacgacca atcaggccat tgatctggta cgaagccgca agcgtcggca ccatgagagt
      901 ctggatgagg gttttctctc cggtacggct gcaacgccgc tgattgcgcc tgccgtcgat
      961 tcccagtcgc tggcacaact gcatgagctg gaagcccata tcagaaaagg gcttgtccat
     1021 ttgtcaccca tgcagcgcaa agtgtttgtt gcccgtcact ttgaagaaag ttctttggcg
     1081 gaaattgctg aagacttgaa ttgcagtgta ggctgtgtca aagtgcattt atttcgcgcg
     1141 gtgcagaaga tgcgcaccca tttgaagccg ctggagccgg atcggtaggc gtgccgggct
     1201 tcaaagcggg tggatatgtt ttttgcggag tatgttctga atgaaattat ttttctttga
     1261 tccggtctct attctttctc tgcgccgtgc ccgccgtgct gccgggcccc tcatggccca
     1321 ggcgctgtat gagccgctct ctcaggctga ggaacacaag ctgcagctgc ttgtggagcg
     1381 gcatcccgag ctggcgcagg agttcgaaag tctgaaagcc tttctacccc ggatcaccct
     1441 tcaggaggat gactggtgcg gcgatctgat gccggcactg cgcgcacaga ttgaagagca
     1501 ggcactgctt cgcagaggcg tattttcccg gcggggcatc aattatgtct ttgtggcttc
     1561 cgcgctgctg ctgggctgcg ggctgtacgc ctttttcatg cttgccgggg acaaccgtac
     1621 cggcaccgtg actccttccc cgcttcggac ggctcagtcc gccagtgcgc ttcagaacga
     1681 gcttctggcg gtgcacgact tcatgaatca gggcaggtac caggaagcgg cagcccgtct
     1741 tgcacaggaa gtgaagctcc accccgatga tcccgcccat gcggatgcgc ttttgcagct
     1801 ggcggatatc cagtacacgc atctgcagca gtatgataag gcctatgaaa gctataaaga
     1861 gctggaacgg agccaccggc cggtttttaa cagccactgg gacagcaacc gccagcttcg
     1921 tctgttgtca gccgcacttc ccctgaattt tcagccactc gcatcgctgg aagcggcggc
     1981 acgggctgag tccccactgc atgcctatga gcacatcctg accgaatacc cggaagagat
     2041 ttggggtgaa gaagcgctgc gtgccatgac gctgctggtc agcgacggat ccggtgaggc
     2101 accggatcag gcttctgcgc tgcaacgtct gtcgggcatg tgcacagagc ctgtcgcact
     2161 ggatcgcatc tgcctggaaa tagggcattg ctacagcgac cggctggcga accgggagca
     2221 ggcggagagt cgtcatctcg ctcttgcgga ctgtgcccgg gaagcgcttg cgcatctgga
     2281 gtaaggtttt tcttggaggc ttccgctctt cttgtggtat cgtattctat gaacggcgct
     2341 attgctttgt ccggcgccgg agaccattgc ggcagccctt tctgaaaata atgggaagtg
     2401 tctgctccgg aatcatacaa ccagggagaa tgcgtgcgtg tccgagccaa tagcctttga
     2461 aaaactgtta gtgcaccggg gcattctgga tgaatcgcgc ctggagcgcg ccaaagggga
     2521 ggcggcgcgc aacgggatgt cactgcttgc tgtgattcct caactggggt atgccactca
     2581 ggaaagcgtg tacgaggcgc ttgccgaatt ctgcgaaatg ccttttgtga tgctgtcccg
     2641 ccgggggatt gaaaaagaaa tcgcacagaa aacgccggcg cgctttgcca cgcattacgg
     2701 ttttgtgccc gtggaggagc gtggcacggc attggtggtg gcgatcagcg accctctgag
     2761 cccgcagttg ctggatgata tccgcatggt gctcaaacgg cgtatagagc ctgttgtgac
     2821 aacgcctgca gagattaccc gtgccatcaa ggaactctat ggtgtcggcg cagacaccat
     2881 ggagaagatg ctgatcgagg ccgagggaga gggagcggta ctcacgcttg aagggagttc
     2941 tgttgagtcg gatctcgg
//
LOCUS       DAOPWB010000224         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_35293_length_2914_cov_4.921301, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000224 DAOPWB010000000
VERSION     DAOPWB010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35293_length_2914_cov_4.921301"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKN92_13030"
     CDS             <1..1154
                     /locus_tag="PKN92_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNZ49761.1"
                     /translation="LLNVRVIRRNDKASYANLIALGPESDSGPRRPSETTGGARPGMR
                     KRRNMLRNPVPPPPPPGGTANKFPLRPRPFAPGPPRWMSHEEYDRLVQSQGIHWFLVT
                     LSIRPMQKDILADLILRLSITGAAWVACAALAAAWQSQKKTAHLAVALIGAEDAATRL
                     QELNTTAAGLMHETKNPLNIIRGMAQIIEQDHALPARTRTQARTITEEADRITGRLNQ
                     FLSFARPQTPQIKSVAVKKLAEDIFSILSADREEKNAVFSLSGQDLNINADEGMLRQM
                     LFNLIHNALQAIGYGGQISVELKQRPDRTLQIDITDSGPGVPAEKKEEIFQPYVSHFD
                     GGTGLGLSIVRRLADAQGWEVRCLPATEGAHFRISGIALSSAHVGGAQA"
     gene            1151..2500
                     /locus_tag="PKN92_13035"
     CDS             1151..2500
                     /locus_tag="PKN92_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNZ49762.1"
                     /translation="MNDRKHILVVDDDPGQRDLLRTFLEKKGSDVTTAASGDEALALL
                     QQGSFSLLISDIRMPGMSGLSLLEALRQICPLLPVLMVTAYPDVRDAVEAMRGGAVDY
                     LEKPIDLEELLSTVSRILGWNADKTAPPAPLPPLPEGIAAQSPALHKVLHEIALVAPS
                     DARVLITGESGSGKEIIADLLHAWSDRKAAPMIKVNCAALPENLLESELFGHEKGAFT
                     SAVARRTGRFEEADGGTLFLDEIGEISPSVQAKLLRVIQDGSYSRVGSNVELKSNARI
                     LAATNRDLENEVVAGRFREDLYYRLNVIEIHMPPLRHHKQDIPLLAARFARELSRGNV
                     RFSAGAMAALETWHWPGNVRELRNAIERAVLLSGGSVILPEHLPRQLIRNAENETGDA
                     PAATLMENVERTAILQALRENNYVRTETARALGISRRTLNYRLRQYKDEGYQIDPES"
     gene            2632..>2914
                     /locus_tag="PKN92_13040"
     CDS             2632..>2914
                     /locus_tag="PKN92_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49763.1"
                     /translation="MYIFIVPILVLSTLVDPISTNSGALRTEILSQEPISIDLQDRKD
                     EPEKERQWFFLYGMVSAYPKLQSERLLKDIFDPIVSTIAPGYDGTRTFTD"
BASE COUNT          701 a          783 c          775 g          655 t
ORIGIN      
        1 cacttttaaa tgtgcgtgtc atccgccgca atgacaaagc ctcttacgcc aatctgattg
       61 cactgggccc ggaatccgac agcggacccc gccgcccctc tgaaacgact ggcggagccc
      121 gcccgggtat gagaaaacgc cggaatatgc tcagaaatcc ggtaccgccg cctccgccgc
      181 cgggaggcac tgcaaacaaa tttccgctcc gcccgcgacc ctttgcccct ggaccgcccc
      241 gctggatgag ccatgaagaa tacgaccggc tcgtccaatc tcagggaata cactggtttc
      301 tggttaccct gtccattcgt ccgatgcaaa aagacattct cgccgatctt attttgcggc
      361 tgtcaattac cggtgccgcc tgggtggcgt gtgcggcatt ggcagctgcc tggcaaagcc
      421 agaaaaaaac ggcgcacctt gccgttgccc tgattggtgc ggaggatgca gcgacccggc
      481 tgcaggaact gaatacaacc gccgccggac ttatgcacga aaccaaaaac ccattgaata
      541 ttatacgggg catggcacaa ataatagagc aggatcatgc gctgcctgca cgtacacgca
      601 cgcaggcacg aacgattaca gaggaagcgg atcgtattac ggggcgttta aatcaatttt
      661 tgagctttgc acgtccccag acaccacaaa taaaaagcgt cgctgtcaaa aaactggcgg
      721 aggatatttt ctccatatta agtgcggatc gggaagaaaa aaacgctgtg ttttctctat
      781 cagggcagga tcttaatata aatgcagatg aaggaatgct ccggcagatg ctctttaacc
      841 ttatacataa tgctcttcag gcgattggct acggcggaca gatttccgta gagttgaaac
      901 aaaggccaga tcggaccctg caaattgaca tcaccgattc cggacccggg gttccggcag
      961 aaaagaagga ggaaattttt cagccctatg tcagtcactt tgacggcggg acgggattgg
     1021 ggctttctat cgtgcgacgc cttgccgatg ctcagggctg ggaagtgcgg tgcctcccgg
     1081 cgacagaagg cgcacatttc agaatcagcg ggattgcgct ttcctctgca cacgtcggag
     1141 gtgcacaagc atgaatgacc gaaagcacat acttgtggtg gatgatgatc cgggccagcg
     1201 ggatctcctt cgaacctttc tcgaaaaaaa agggagcgat gtcaccacag ccgcttcggg
     1261 cgatgaagca cttgctctgc tccagcaggg cagtttctcc cttcttatat ccgatatccg
     1321 tatgccgggc atgtccgggc tttctttgct tgaagcgtta aggcagatct gccctttgct
     1381 gcctgtgctg atggttactg cttatcccga cgtgcgtgac gccgtggaag ccatgcgtgg
     1441 cggcgctgtc gattatcttg aaaagcccat tgatctggaa gaacttctct ctactgtcag
     1501 cagaattctg ggatggaatg cggataaaac ggcgcccccc gctcccctcc cgccgctgcc
     1561 ggaaggtatt gccgcacaaa gccccgccct gcataaagta ctgcacgaaa ttgcgctggt
     1621 tgcgccctct gatgcccggg tactgattac cggagaaagc ggctcgggca aagaaattat
     1681 cgccgacctt ctgcatgcct ggagcgacag aaaagccgcg ccgatgatca aagtgaactg
     1741 cgccgccctt ccggaaaacc ttctggaaag cgagctcttc ggtcatgaaa aaggggcgtt
     1801 caccagcgct gtcgcacgcc ggactgggcg ctttgaagag gcggacggcg gcacgctttt
     1861 tctggatgaa atcggagaga tttctccttc cgttcaggcg aaattgctgc gggtaattca
     1921 ggacggctcc tacagccgtg tcggatccaa tgttgagctg aaaagcaatg ccagaatttt
     1981 agctgctaca aaccgggatc tggaaaatga agtggtagcg ggacgttttc gcgaagacct
     2041 gtattatcgg ctcaatgtta ttgaaattca catgccgcct ttgcgacatc ataaacagga
     2101 tattcctctg ctggctgccc ggtttgcccg ggaactctcc cgtggaaatg tccgtttttc
     2161 agcgggggcg atggcggcat tggaaacctg gcattggccc ggaaatgtgc gtgaactgcg
     2221 taatgccatt gaaagagctg tgctcctgtc cggcggctct gtaattcttc cggagcacct
     2281 gccgcggcag ctgatccgga acgccgaaaa tgaaaccgga gacgcaccgg ccgcaaccct
     2341 catggaaaac gtggagcgaa ccgctattct gcaggcactg cgggaaaata attatgtgcg
     2401 cacagaaaca gcccgtgcgt tggggatcag tcggcgcacc ctgaactaca gacttcgcca
     2461 gtataaggac gaaggctatc agattgaccc ggaatcttaa ggatcctgcc acagagtggc
     2521 gcaaagccag ttgacccggc aaaacctgtt ttttgcagat ggcaattgcc ctctcataat
     2581 tggtaggata actcctctcc cgcaccccta tgttttcatt ggttgtttat tatgtacatt
     2641 tttatagtac ctattttagt tttaagtact ctggttgatc cgataagcac gaatagcggc
     2701 gcccttcgca cagaaatcct gagccaggaa cctatatcca ttgaccttca ggacaggaaa
     2761 gacgagcctg aaaaagaacg gcaatggttc tttctttacg gcatggtcag tgcttatccg
     2821 aaactgcaga gcgaacggct tctgaaagat atttttgatc ctatcgtatc caccatcgca
     2881 cccggttatg atgggacccg cacttttacc gatc
//
LOCUS       DAOPWB010000225         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_35881_length_2880_cov_5.517522, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000225 DAOPWB010000000
VERSION     DAOPWB010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35881_length_2880_cov_5.517522"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="PKN92_13045"
     CDS             complement(<1..1691)
                     /locus_tag="PKN92_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49764.1"
                     /translation="MTGKVQNKQGMALILVLLSTIIILGAVFVVSQTVLSSKSQTDLT
                     TQMHLAEEAAKSGIDLAVEHLWNQYVVGRGNTTGNLASYRSFLDDVVKKNQTKFLVNP
                     DMPTVIDTETNSQVLSLSLRREDTLNGIQITAEAVGEAQGYRQRAVQTMLVSGAQFAG
                     YEYAVLANYINCILCHASFYNIEERLNTSAAKYNTFDRIKLATLEALLFRPAGADSVV
                     AGTIYTRGQVYNEAYQAINATTIKNSSMKHFEFSKTDGKLKQSATGTMTKSSLTQAGL
                     DRNGNLEQFASLYTNYPTDAKQMTDGILPETFPAPFPDNNGNRHVDPDEFEEVAELLE
                     GRLTGGIAYGVPDGGSYNSKNLPASSNSALADLSNTGRYDGNLILVGTDANPIVLNGE
                     IAVNGDLILQGKVKGWGQFFVKGNTYLTGDVTYADAPGKFGEAADGTRNGLALVSGGN
                     ILMGDYLTVRGKNYSNDTAKFPDSSNSIQVRTRSLKKTKKINNKNETIDIGYFSPGVS
                     DAGEVQAQMRNEQGKLVPRDGQQFSFTQSELKLFNNLELDKALADPNYRPRFYGLR"
     gene            complement(1725..2345)
                     /locus_tag="PKN92_13050"
     CDS             complement(1725..2345)
                     /locus_tag="PKN92_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ49765.1"
                     /translation="MNKSGFTLLELTISTALLVIIFSLGLVAMKTSSASVSLNRGKSQ
                     LQEEARRLMLVLTQELEQAIKPAPQGTTLPYGAKALTIINGGQGIRFQIPANPAFTAF
                     SAPIEYRFQTEDTPVAGGLFPFGNAWLDPGEDSNNDGILNRNIVRVQGGQTRALGAAN
                     SIADATFELLENGNLLRISLVLTAPIGDTRSQLVTYEFQRDIYLMN"
     gene            complement(2361..>2880)
                     /locus_tag="PKN92_13055"
     CDS             complement(2361..>2880)
                     /locus_tag="PKN92_13055"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNZ49766.1"
                     /translation="KFFTKHAMQIRGFTLVEIMFAMGIFILLMWSVAHSLVYSYAVLE
                     IQEQRNTALASCQAVLAAMRELSYNTQESADCTGGRPVFPCVLLNYSNSFPETLEGAS
                     AAVLNQYGSFFTLREQQFQLEMRDDDGAPAQTSVVAAMNTNPVYVTVTTTWLGARNHR
                     FTVSASAIITNS"
BASE COUNT          656 a          700 c          774 g          750 t
ORIGIN      
        1 cgcagtccgt aaaaacgcgg gcgatagttg ggatctgcca gcgctttatc cagttccaga
       61 ttgttaaaaa gtttcagttc cgactgggta aagctgaact gctgtccgtc acgtggtacc
      121 agcttccctt gttcattccg catttgggcc tgcacttcgc ctgcatcgct gacaccgggt
      181 gaaaaatagc ctatgtctat tgtctcgttc ttgttattga tttttttggt ttttttcagg
      241 cttcgtgttc tgacctggat actgttggag gaatcaggaa acttagctgt atcgttgctg
      301 tagtttttac ctcgaactgt caggtaatcg cccatgagaa tattaccgcc ggaaacgagt
      361 gcgagtccgt tgcgtgtacc gtcagccgct tcgccgaatt tgccgggcgc atctgcatag
      421 gtgacgtcac cggtcagata ggtgtttcct tttacaaaga actgacccca gcccttcacc
      481 ttgccctgaa gaatgaggtc gccgtttaca gcgatttcac cattgagcac aatggggttg
      541 gcatcggtgc cgacaagaat cagattgccg tcataacgcc ctgtattgga aaggtctgcc
      601 agggcgctgt tggaggaagc gggcagattt ttgctgttat aggaaccgcc atccggcaca
      661 ccgtaagcga tgccgccggt gaggcgccct tcaagcaatt cagccacttc ttcgaactca
      721 tcaggatcca catggcgatt gccgttgttg tccgggaagg gtgccgggaa ggtttccgga
      781 aggattccgt cggtcatttg tttcgcatct gtagggtagt tggtatagag gctggcaaac
      841 tgctccagat tcccgtttct gtccaagccg gcctgtgtca ggctggactt ggtcatggtt
      901 cctgtcgctg actgtttcag ttttccatcg gttttactga attcaaaatg tttcatggag
      961 ctgtttttaa tggtagtggc attgatcgcc tgataggctt cgttatatac ctgaccgcgg
     1021 gtatagatgg tgcccgccac tacggagtcg gcgccggccg gacggaagag cagcgcttcc
     1081 agcgtagcca gcttgatgcg gtcgaaggta ttgtattttg ccgcagacgt attgagccgc
     1141 tcttcaatat tgtagaaaga agcatggcac aaaatgcagt tgatgtaatt tgccagcacg
     1201 gcatattcat aaccggcaaa ctgggcgccg ctgacgagca tggtctgcac ggcacgctga
     1261 cggtaaccct gcgcttcacc gacggcttct gctgtgatct ggatgccgtt gagtgtgtct
     1321 tcccgtctga gagaaaggga gagaacctgg ctgtttgttt ccgtgtcaat tacagtaggc
     1381 atatcgggat taaccaggaa ttttgtctgg ttttttttga caacatcgtc aagaaaagag
     1441 cgataggagg caagattgcc tgtggtgttg cctcgaccga caacatactg gttccacagg
     1501 tgctcaacgg ctaaatctat ccctgatttt gccgcttcct ctgccagatg catctgtgtt
     1561 gtcagatccg tctgggattt ggaagacagc accgtctggc tgacgacaaa aaccgcaccc
     1621 agaataataa tggtcgaaag cagaacaaga attaaagcca tgccctgctt gttttgtact
     1681 ttgccagtca tggttttgcg ctccttacat gttttttgct gcattcagtt catcagataa
     1741 atgtcgcgct ggaattcata ggttaccagt tggctgcggg tatcgcctat gggcgccgtc
     1801 aataccagcg aaatccttag aaggttgccg ttttccaaca gctcgaaagt ggcatcagca
     1861 atggaattcg ccgctccgag agcccgtgtt tggccacctt gtacccggac aatattccgg
     1921 ttcagaatac catcgttgtt gctgtcttca cccggatcga gccaggcatt gccgaaaggg
     1981 aaaagacctc ccgcaacagg ggtgtcttct gtttgaaacc gatattcaat gggggcacta
     2041 aaagcagtaa aggcgggatt ggcgggaatc tgaaagcgaa tcccctgtcc gccattaata
     2101 atggtgagtg ccttggctcc gtaggggagc gtagtgccct ggggtgccgg tttgatcgcc
     2161 tgctccagtt cctgtgtcag cacaagcata agccgccgcg cttcttcctg caattgagac
     2221 ttgcctctgt tcaggctgac ggaagcggat gaagtcttca tggcaacaag ccccagcgaa
     2281 aaaataatga cgagaagggc tgttgaaatc gtgagctcca gcaatgtgaa cccggattta
     2341 ttcaatggga cctcctttcc ctaactgttg gtaatgatgg cggaagcact gacggtaaag
     2401 cgatggtttc gtgcgcccag ccaggtggtg gtcaccgtta cataaacagg atttgtattc
     2461 attgccgcaa cgacgcttgt ctgtgcaggg gcgccatcgt catcccgcat ttccagctga
     2521 aactgctgtt cgcgcagggt aaaaaaagaa ccgtactggt tgaggacagc agccgaggct
     2581 ccctccagcg tttcaggaaa gctattggag taattgagga gtacgcaggg aaatacaggc
     2641 ctgccgccgg tgcagtcagc actttcttgc gtgttgtacg aaagttcccg catggctgca
     2701 agtacagcct gacagctggc aagtgcggtg ttgcgctgct cctgaatttc gagaacagca
     2761 taggagtaaa caaggctatg tgcaacagac cacatgagca atataaagat gcccatggca
     2821 aacataattt cgaccagcgt aaaacccctg atctgcattg catgctttgt aaagaatttt
//
LOCUS       DAOPWB010000226         2875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_35983_length_2875_cov_5.421277, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000226 DAOPWB010000000
VERSION     DAOPWB010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35983_length_2875_cov_5.421277"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1337)
                     /locus_tag="PKN92_13060"
     CDS             complement(144..1337)
                     /locus_tag="PKN92_13060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 domain-containing metallophosphoesterase
                     family protein"
                     /protein_id="HNZ49767.1"
                     /translation="MFFVSPHYRCSLVIAATMTLILGTACGFASEGKERGNIHITHGP
                     VLQQPQRNEITVTWHTSKPALCEVRYGEGDTLNKRAVASSLGLIPNDSTCHAVRLTGL
                     TPGATVRYQLISKAMRNFHNPYVPVFGKTVESEIFTYRHLDPEKKKFSFVMWNDIHDD
                     CERLEAMFKDVDWKDIDFVVMNGDIVNDFKSDEQFFNAFYDACARQFGSSIPLVYVRG
                     NHETRGAWARRFFDYVPGVSGRSYYAFTHGGVYFLALDSGEDKPDDHNEYAGMVDFVR
                     FRDEETAWLLDELKSEAWKKVPYKVVISHQPCATSEEDWFGEQEIRRLWRGPLNEIGT
                     QLWLCGHTHRFSWCRPGENGDNRFHLMTNPTDGTVRVDVSPEAMEVTVIRKGGEILHK
                     ESIVP"
     gene            complement(1484..2413)
                     /locus_tag="PKN92_13065"
     CDS             complement(1484..2413)
                     /locus_tag="PKN92_13065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNZ49768.1"
                     /translation="MSEDTLKISVVIPIFNEERSLAELYERITATLEQMGQPYEVIAV
                     NDGSTDSSVDILKDIRKKDPRWRILLLSRNFGQSPALYAGFSQAQGDYVFMLDADLQV
                     YPEDIPILYEKLEAGYDMVGGWRVNRHDNPFRKIMSRFLNRYTGHATGFPLHDHGCSL
                     KGFRRQLVKHMTEFSHRCRYLPVDAAMLGGKVTEVKVRHARRQYGSSRYSLVKLFRTG
                     FDLITTVTILPLQTIGVLGVLSAGAGVLLTLHGIISGLMNDNVSQLEIILAAFLFFGG
                     VHLCALGIVCEYVGRIYIETQRKPLFIIQEEVE"
     gene            complement(2535..>2875)
                     /locus_tag="PKN92_13070"
     CDS             complement(2535..>2875)
                     /locus_tag="PKN92_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNZ49769.1"
                     /translation="KEHNPNIRLIAVEPKNVSALLGHEPGLHQIQGIGDGFIPAVLDV
                     SMIDEVVEVTDEDAIRTTRELGTEHGLLVGISSGANVWAARQAAPHIRGNVVTVLPDR
                     AERYFSTALM"
BASE COUNT          687 a          757 c          782 g          649 t
ORIGIN      
        1 gaatgacgag agcggaaccg gaatgacgga ggcgagggaa gcagggatga cgtgggggga
       61 accgagggaa gcagggatga cgtgggagga gcaggaatga cggaggggct ttaaatgttc
      121 gggccggttc ctagtcctgc tgatcagggg acaatacttt ccttgtggag gatttcgccg
      181 cctttacgga tgaccgtgac ctccatggct tcgggagaaa catcgacgcg gacagtgccg
      241 tcggtgggat tggtcatcag gtggaagcgg ttgtctccgt tttcaccggg gcggcaccag
      301 ctgaagcgat gggtatgtcc gcagagccag agctgcgtcc cgatctcatt gagggggcca
      361 cgccagagcc gccgtatttc ctgttcgccg aaccagtctt cctcggaagt ggcgcagggc
      421 tgatgggaga tcaccacttt ataaggcacc tttttccacg cctctgattt gagttcgtcg
      481 aggagccatg ccgtttcttc atctctgaat cggacaaagt caaccatacc ggcgtattca
      541 ttatgatcat caggtttgtc ttctcctgaa tcaagtgcga ggaaatagac accgccgtgc
      601 gtaaaagcgt aatagctgcg cccgctgacc ccggggacat aatcgaaaaa gcgacgtgcc
      661 caggcgcccc gggtctcatg attgccccgc acatacacca ggggaatgga cgacccgaac
      721 tggcgtgcgc aggcgtcgta gaaggcattg aaaaactgtt catcgctttt gaagtcattg
      781 acaatatcgc cgttcataac cacgaagtcg atgtctttcc agtccacgtc cttgaacatg
      841 gcttccagtc gctcgcaatc gtcgtgaatg tcattccaca tgacaaaaga aaactttttc
      901 ttttcaggat cgagatgccg gtaggtaaag atttcacttt ccaccgtttt tccgaacaca
      961 gggacatagg gattatgaaa gttgcgcata gccttggata tcagctgata gcggacggtg
     1021 gcgcctggcg ttaatccggt caaccgcaca gcatggcagg tactgtcgtt agggatcagc
     1081 ccgagactgc ttgccacagc ccgtttgttg agggtatccc cttcaccgta acgcacttcg
     1141 cacagagcgg gcttgctggt atgccaggtg actgtaattt cattgcgctg gggctgctgc
     1201 aatacagggc catgggtgat gtgaatattg ccccgttctt ttccttcgga agcgaagccg
     1261 caggcggtgc ccagaatcaa agtcattgtt gcggcaataa cgagggaaca gcggtaatgc
     1321 ggggacacaa agaacatcgc acaagccttt ctttattttt tttggaagca caaaagtacc
     1381 cgtagcaaac aaagaaagtg cgttttcttt ggaccaggca ccgggcacct tcaaacctgc
     1441 tcttcagtat acccaagggc atggagaaaa taaaacggcg gaattattcg acttcttcct
     1501 gaatgataaa aagaggcttg cgctgcgttt caatatagat gcgacccacg tactcgcata
     1561 caatgccgag tgcacacagg tgtacgccgc caaaaaaaag aaacgccgca agaataattt
     1621 cgagctggga aacattgtcg ttcatcaatc cggaaataat tccatgcagg gtaagcagca
     1681 cgcctgcacc cgcactgagc acacccagca cgccaatggt ctgcagaggc aggatcgtca
     1741 cggtggtgat cagatcgaaa ccggttcgaa acagtttgac caggctgtac cggctggatc
     1801 cgtactggcg acgggcatgg cgcactttga cttccgtcac cttgcctccc agcatggcgg
     1861 catcgacagg gagatagcgg cagcgatggg aaaattccgt catgtgctta accagctgcc
     1921 tccggaaacc cttgagcgaa cagccgtggt catgaagcgg gaacccggtg gcatgtcctg
     1981 tatagcgatt cagaaatcgc gacataattt tccggaaggg attgtcatgc cggttgaccc
     2041 gccatccgcc caccatgtcg taccctgcct ccagtttttc ataaaggatc gggatatctt
     2101 ccggatacac ctgcagatcc gcatcgagca taaagacata gtcaccctgc gcctgcgaga
     2161 atccggcata aagtgcgggg ctctgcccga agttcctgga gagaagaagg atgcgccagc
     2221 gcggatcttt tttccggata tctttgagaa tatccaccga gctatctgta cttccatcat
     2281 tgacggcgat aacttcatag ggctgcccca tctgctccag cgtggcagtg atccgctcat
     2341 aaagctccgc cagactccgt tcttcattaa agatagggat gaccacggat attttcagtg
     2401 tgtcttcgct catgaacgtg cttcctcatt ggcattatgg ggaactggga gccgatcttc
     2461 cattgatctg cttcttttga aaggcatcat atcggaagtg aacaaaggag tcaacaaaaa
     2521 tgtctgtttg cggtttacat aagcgctgtg ctgaaatacc gctccgcacg gtcgggcagg
     2581 acggtaacaa cattgccccg gatatgggga gccgcctgcc gtgccgccca aacgttggca
     2641 cctgaggaaa tccctaccag cagaccatgt tccgtgccca gttctctggt tgtccggatc
     2701 gcgtcttcat cggtcacctc cacgacctca tcgatcatgg acacatcgag tacagcgggg
     2761 ataaatccgt caccgatccc ctgaatctgg tgcagcccgg gctcgtgccc cagcagtgcc
     2821 gacacgtttt tcggctccac ggcaatgagc cggatgttcg gattatgttc tttaa
//
LOCUS       DAOPWB010000227         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_36059_length_2871_cov_4.247159, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000227 DAOPWB010000000
VERSION     DAOPWB010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36059_length_2871_cov_4.247159"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKN92_13075"
     CDS             <1..470
                     /locus_tag="PKN92_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49770.1"
                     /translation="ITSIIPDVTINPADEVNAQFGWTLDALLNTQNIGALLGSPVEGM
                     LPAVDKDVKVPAGFGNGGAGSASDTVYRLREGIERFLITDINNPAASALAQSTVYIMF
                     DQISTNPADFNHVPGGSNILYMDGHVAFQRYEEKGNGPVNGPMARLIGLLTGM"
     gene            complement(535..2724)
                     /locus_tag="PKN92_13080"
     CDS             complement(535..2724)
                     /locus_tag="PKN92_13080"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HNZ49771.1"
                     /translation="MKTRLFATALFLCLLLLPLQLFADDTLECISGDLALTFHQGSGG
                     IRITRLQDLGQDRNLLAALCRPLFVLVVCDQDGKETHVPSTDGWERVGMEATDGGALL
                     RWEDYPGADLSTFTVEVRLYARPEESAWEWHIATAGALNTTRLEDVVFPQITLHESIC
                     DGGILYPDGPGALIQYHWDTDMQKWQRYGGGWGSMQFMSTFSRWSPEQNQSGFYFAMH
                     DPQGSAKEIVAQSKAKERTVSLFFEHPIPDTTRTNNYSLPGKAVWRLFRGDWFDAAQY
                     YKNWVQKNALWMPRLGREGREDTPLWMRELCVWAQTGGTPEEVTERVKSFADYLDVPV
                     GFHWYNWHEIPFDNDYPHYFPAKEGFAEAVQALQAANVYVMPYINGRLWDTHDCGAED
                     CSFTSLALPAVTKEEDGAPYIEKYGSRESDGSQVALGVMCPATPLWQNKVKEIVLRLQ
                     KECGVKGVYIDQIAAATPALCMDPSHGHPLAGGSWWNRSYWNMLDRIRREMPEGHVIT
                     TECNGEPYINRFDGYLSWHWQTDGQLPVFPAIYGGTIQCFGRSYGGGETASLALRMKA
                     AQQLVFGEQIGWINPDLAMAEENAAFFKQIVQLRWLLRRYFYAGEMLRPPVTGGTIPQ
                     VRADWQWHGEHPVITDALLTGAWQIAGENRTALLFANVSDEPIEAPIVLRNTGTDGAP
                     LARTVLTDKSTAPVPDCITSGEPVMLTLPPRKALAWEFGEAIRYSPR"
BASE COUNT          581 a          845 c          812 g          633 t
ORIGIN      
        1 ccattacctc cattattccc gatgtgacca tcaacccggc ggatgaagtc aacgctcagt
       61 tcggctggac tctggacgct ctgttgaaca cccagaatat cggtgctctg cttggttccc
      121 ccgtcgaagg tatgcttccg gcagtggata aagacgtgaa agtgcctgcc ggtttcggta
      181 atggcggtgc cggcagtgcc agcgacacgg tctatcgtct gcgcgaaggt attgagcgct
      241 tcctgatcac ggacatcaac aaccctgcag ccagtgccct ggcacagagc acggtttaca
      301 tcatgttcga ccagatctcc accaatcctg ccgacttcaa ccacgtgccc ggcggctcca
      361 acatcctgta catggatggt cacgttgcct tccagcgcta tgaagaaaaa ggtaacggcc
      421 ccgtcaatgg tccgatggcc cgtctgattg gactgctgac cggcatgtaa gccgacccct
      481 tttctgctga ttatctgcgc ctcccggagt ttccgggagg cgcttttttt tgttttaacg
      541 ggggctgtaa cggatcgctt cgccaaactc ccaggcgaga gcctttcggg gcggcagggt
      601 gagcatgacc ggctctcctg aagtgatgca gtccggaacg ggtgccgtgc ttttatctgt
      661 gagcactgtc cgggcaaggg gtgcaccgtc tgtaccggtg tttctgagta cgatgggcgc
      721 ttctatcggt tcatcggaca cattggcaaa gagcagtgcc gtcctgtttt ctccggctat
      781 ctgccaggcg cccgtaagca gcgcatcggt aatgaccgga tgctctccat gccactgcca
      841 gtcggcgcgc acctgcggta tagtgccgcc ggtcacgggc ggccgcagca tttcgcctgc
      901 ataaaaatac cggcgcagca gccagcgcag ctgcacgatc tgtttgaaaa aggcggcgtt
      961 ttcttccgcc attgccagat caggattgat ccatccgatc tgttcgccga agaccagctg
     1021 ctgcgccgcc ttcattcgga gggcgagact tgccgtctca ccgccgccat aggacctgcc
     1081 gaagcattga atcgtaccgc cgtagatcgc aggaaaaacg ggtagctgcc catcggtctg
     1141 ccagtgccag ctgagatatc cgtcgaagcg gttgatatag ggttcgccgt tgcactccgt
     1201 agtgatcaca tgcccttccg gcatttcccg gcggatccgg tccagcatgt tccagtagct
     1261 ccggttccac caggagccac ccgcaagagg atggccgtgg gacggatcca tgcacagggc
     1321 aggcgtcgct gcggcaatct gatcaatata cacgcccttc acgccgcact ctttctgcag
     1381 gcgcagcacg atttccttca ctttattctg ccacagcggt gtggcggggc acatgacccc
     1441 cagcgcaact tgggatccgt cgctttcccg gctgccgtat ttttcgatat aaggcgcgcc
     1501 atcttcttct ttggtcacag cgggcaaagc aagcgaagtg aaggagcagt cttccgcacc
     1561 gcagtcgtgc gtatcccaga gccgtccgtt gatgtagggc ataacgtata cattggctgc
     1621 ctgcagggcc tgcaccgcct cggcaaatcc ctctttggca gggaaataat gaggataatc
     1681 attgtcgaag gggatttcat gccagttgta ccagtgaaag cctacgggca catcgaggta
     1741 gtcggcaaaa gacttgacgc gctccgtcac ttcttccggt gtgcctcccg tctgtgccca
     1801 tacgcagagc tcacgcatcc aaagaggcgt atcttcccgc ccttctctgc cgagccgggg
     1861 catccacagc gcattctttt gcacccagtt tttgtaatac tgcgccgcat cgaaccagtc
     1921 gccccggaag agccgccaca cggctttgcc cggaaggctg taattattgg ttcgggttgt
     1981 atcgggtatg ggatgttcga aaaagaggct gactgtccgc tctttcgctt tcgactgggc
     2041 aacgatttct tttgccgatc cctgtggatc atgcatggca aaatagaaac cggactggtt
     2101 ctgttcgggc gaccacctgg aaaaggtgct cataaactgc atgctgcccc agccgccgcc
     2161 gtagcgctgc catttctgca tgtccgtatc ccaatgatat tgaatcagcg caccgggccc
     2221 gtccggatag aggatgcccc cgtcacagat ggattcgtgg agggttatct gcggaaaaac
     2281 gacatcttcc agccgtgttg tattgagtgc cccggctgtg gcaatgtgcc attcccaggc
     2341 gctttcttcg gggcgggcat agagccgcac ttccacggta aaggtgctga gatccgcacc
     2401 gggatagtcc tcccaccgga gcagggcgcc tccatccgtc gcttccatgc cgacccgttc
     2461 ccatccgtct gtggagggga catgtgtctc ttttccgtcc tgatcgcaga caacaagcac
     2521 aaagaggggc cggcacaatg ccgccagaag atttctgtcc tgacccaggt cctgcagacg
     2581 ggtgatccgg atgccgccgg agccctgatg aaaggtaagg gcaaggtcgc cggaaataca
     2641 ttccagcgtg tcgtcggcga aaagctgcaa cggaagcagc agcaggcaga gaaagagcgc
     2701 tgtggcaaag agacgcgttt tcatggcaca ctcctgtcgg ctcatccatg cagcggcgct
     2761 gcagggttgt aggcggacag cctgctgatg ctgtaaaaag ctgtccttta aaaatcgatg
     2821 acgctgagct ccggaataca ggaatccagc ggaagggtct caatatgggt c
//
LOCUS       DAOPWB010000228         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_36300_length_2858_cov_5.208348, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000228 DAOPWB010000000
VERSION     DAOPWB010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36300_length_2858_cov_5.208348"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..774
                     /locus_tag="PKN92_13085"
     CDS             355..774
                     /locus_tag="PKN92_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNZ49772.1"
                     /translation="MLLKKLTIVLVDDDADVLESVAACFHRLGHDVIPFSNALRALDH
                     LHAGACDLLVADIKLREMSGLDLISQVRARHPLMAIVAMTGHEEEYPLSMALKAGADA
                     YLAKPFSLKTLELMLQQTFWETASRLDWWEHHSVSNC"
     gene            complement(798..2246)
                     /locus_tag="PKN92_13090"
     CDS             complement(798..2246)
                     /locus_tag="PKN92_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49773.1"
                     /translation="MLYLLLIFAVLGASEGEVLSEPPALSAPSALSEPVAEPTAYGLP
                     SGETVVAPEGMLDALKALGTAPLPEEQPWLCAYVEKEPLDLAARLFGISQYYETNAFT
                     AQHGTNLHAHIKFGSADAEKMARFAGSFAAEGIRRYWTQSLAGTALSPSLILPFMESL
                     YNDTPEMIRKAFASGIEDVFASGPLPLTEVLHPGSLYGEGLLLDAWQHCVTLSAFSKG
                     FDIFRWIDMPRIQALFFKSTGVWLFGGEALNPEQLAILESVFTSIPFSLHGIAAAFVQ
                     EGIPAVNPENALRIPGYTLRVPVLDLSLMRDATLLPPLLSLPPLPEYTALLLEQVAQA
                     VTSFQLIKRPELMQRCFHVLDWVRKRPEAGLEQIVPGALMPASPDRLVTYLVYLWLIN
                     SDMWLDGALALYVQHLAAPFLSLLLAADVFSEGSDVTRLLHTTSAGKVYSEQTAVRRT
                     EWAGVGNVVTAVALRGQLWHYEGDTPPVPWSF"
     gene            2442..>2858
                     /locus_tag="PKN92_13095"
     CDS             2442..>2858
                     /locus_tag="PKN92_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49774.1"
                     /translation="MRTLFFMSLCLGWIFLSPFHARAAEPMSIGSRLEVFADARMIES
                     LENLQHVPGTPVHRETVLVFDRPWEGRYCGYVTVLQDGDLYRMYYRGLPEAGRDGSAR
                     EVTCYAESKDGIRWEKPENNIVLADQPPYSHNFSPFI"
BASE COUNT          748 a          772 c          694 g          644 t
ORIGIN      
        1 ccaaacgatg accccgtttg tccaaataat ttacagaaac tatggtacgt tatccttatt
       61 cagatttctt attaaaaagg tagaattata tgcgtttgca aggaaaaaca cagcattgtt
      121 accctatatg tgacgcacca ggctgaaaac aacgtgaatt tcttttcaaa agcagcattt
      181 aaaaaagaaa aaaaaataga aataattcgg aatacattac tccattttta aaaagcgctc
      241 ttgactctta aggatggctt atactagtat tctccgtgta gtttcatgca acgggcagcc
      301 ccctatatcg cacccgcttc tttttacacg ccatcatgtc ggagatataa agcgttgctg
      361 ctgaaaaaac tcactattgt acttgtagat gatgacgccg atgtgctcga gtctgtagcg
      421 gcatgcttcc atagactcgg gcatgatgtt atccctttca gcaatgcgct gagggcgctg
      481 gatcacctgc acgcaggcgc atgcgatctt cttgtggcgg atatcaaatt gcgggaaatg
      541 agcggtcttg atctgatcag tcaggtgaga gcacgtcatc ccctcatggc aattgttgcc
      601 atgaccggcc atgaagaaga gtaccctctc tccatggcgt tgaaagcagg cgctgatgcc
      661 tatctcgcca agcccttcag tctgaaaacg ctggagctga tgttgcaaca gactttctgg
      721 gaaacggcat cccggctgga ctggtgggaa caccattctg tcagcaactg ctgaatcctc
      781 ttctgcagat acacgcctca gaaagaccac ggtaccggcg gtgtatcccc ttcgtaatgc
      841 cacaactgcc cgcgcagtgc aactgcagtc accacattac ccacgcccgc ccattccgtg
      901 cgccgcaccg cagtctgttc actgtatact tttcctgctg aagtcgtatg cagcagccgt
      961 gtgacatctg aaccttccga gaagacatct gctgcgagca gaagcgaaag aaaaggggct
     1021 gccagatgct gcacgtacag cgcaagtgct ccatccagcc acatgtcgct attaatcagc
     1081 caaagataaa ccagatacgt caccaaccga tccggcgaag caggcatcag cgccccgggc
     1141 acgatctgtt ccaatcctgc ttccggtctt ttccgtaccc agtcaagcac atgaaagcac
     1201 cgctgcatca gttcaggccg tttgatcagc tgaaaactcg tgacagcctg cgccacctgc
     1261 tccagcagca aagcggtata ttcaggcagc gggggcaacg aaagcagcgg aggaagcagc
     1321 gttgcgtccc gcatcagtga caaatccagc acaggcaccc gcagcgtata gcccggtatt
     1381 cgcaaagcat tttccggatt gaccgccgga atcccctcct gtacaaaagc ggcggcaatt
     1441 ccatgcagtg aaaagggaat cgatgtaaaa acactttcaa gaattgccag ctgctccgga
     1501 ttcaatgctt ccccgccaaa gagccagaca ccggtgcttt tgaagaaaag cgcctgaata
     1561 cggggcatgt caatccagcg gaaaatatca aatccctttg aaaaggcact taaagtgacg
     1621 cagtgctgcc aggcatccag cagcagtccc tcgccataaa ggctgccggg gtgcagcact
     1681 tcagtcagcg gaagcggacc ggaagcaaac acatcttcaa taccggaagc aaaagctttt
     1741 cgaatcattt caggtgtgtc gttgtaaaga gactccataa aaggaaggat aagcgacgga
     1801 gaaagcgctg taccggcaag agactgcgtc cagtaacggc ggattccttc ggcggcaaaa
     1861 gaaccggcaa aacgtgccat cttttctgca tcggcggaac cgaactttat atgagcatgc
     1921 aaattggtgc cgtgctgcgc tgtaaaagca tttgtttcat agtactgact tatgccgaac
     1981 agacgggcgg caagatcaag aggctccttt tccacataag cacagagcca gggctgctct
     2041 tccggaagag gcgccgtgcc aagtgctttc agtgcatcca gcatgccttc cggcgccact
     2101 acagtttcac cagacggcag cccgtaagcc gtcggctcag ccaccggttc tgatagagcg
     2161 gacggcgcag acagggcagg cggctcagac agcacctctc cctccgacgc tcccagcacc
     2221 gcaaatataa gcaataaata aagcatttca gtatctccgc tcagcaaaag aattctgaaa
     2281 gagcatacca tatttttaaa taagaaggtg ggcgcgcggc acaacctgcc cgaaaacagc
     2341 ggacatgaac acgttgtccc gttttccttt ttaaatacag tgcgctacaa tggtttctgc
     2401 tccgttcttc tgcgaaccgt ttttggtaaa aggaaaaagt aatgagaacg ctgtttttca
     2461 tgtccctctg cctcggctgg atatttctct caccctttca tgcccgggcg gctgaaccca
     2521 tgtccatagg ttcacgcctg gaagtttttg ccgatgcccg gatgatcgaa tctctcgaaa
     2581 acctgcagca tgtgccgggg acgcctgttc atcgcgaaac agtgcttgtt tttgatcgtc
     2641 cctgggaagg caggtattgc ggttatgtca ctgtcctgca ggacggtgac ctttatcgca
     2701 tgtactacag agggttgcct gaagcgggca gggacggttc cgcccgggag gtcacctgtt
     2761 acgcggaaag caaggacggc atccgctggg aaaagcctga aaataatatt gtccttgccg
     2821 atcagcctcc ttactcgcat aatttcagcc ctttcatc
//
LOCUS       DAOPWB010000229         2796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_37389_length_2796_cov_7.376870, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000229 DAOPWB010000000
VERSION     DAOPWB010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37389_length_2796_cov_7.376870"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="PKN92_13100"
     CDS             complement(<1..1426)
                     /locus_tag="PKN92_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNZ49775.1"
                     /translation="MAVLDSGVDKTHSFFPAGKVVAEACFSTTDAGQHAETVCPDGNE
                     SQTGAGAGIQCGGANGTEIDGCKHGTHVAGIAAGKSASINGVAKEANIIAIQVFSKIT
                     DAASCSSYGLTSPCALSFTSDQNLALEHVYSLKNTYNIAAVNISIGGGQYFTACDTSE
                     SSTKAVIDNLRSAEIATVISSGNEGYKNSVSSPACVSSAVSVGATTKADAEADYSNYH
                     PVMLSLFAPGSAINSSVPGGGWDSWDGTSMAAPHVAGAWAIIKQKWPTASVANVLKGL
                     QDTGVAVTLKAGDMAGGSIKRINVLAALNVNLALNMPTGLSGSSVSTSGIDLSWTDNS
                     TGETGFKVDRKTGASGTYSQVATVGAGTVTYSDTGLSEGKTYYYRVSAYNAEGDSPDS
                     NEASAVTMLGAPSGLIASAVSYTQINLSWTDNSGAESEFIISRAGVSGGPYTEIATVS
                     ANSTSYSDTGLTFGTTYYYRVQAVN"
     gene            complement(1520..1834)
                     /locus_tag="PKN92_13105"
     CDS             complement(1520..1834)
                     /locus_tag="PKN92_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49776.1"
                     /translation="MAAEGQGSQKASPALLAKVQKAGSVRVIVRLNTSFQPEGQLSGP
                     TAVAGQKARIASTQARLQQEFSKHNVRGIKKFKHIPYTAMAVDSNALDALIANPLVVS
                     IE"
     gene            complement(2014..2472)
                     /locus_tag="PKN92_13110"
     CDS             complement(2014..2472)
                     /locus_tag="PKN92_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49777.1"
                     /translation="MLKKRFLKIWPCLASLFLMILPGCSQIVNAHNLEANPPVSQNES
                     TSSVKDYSRLLTVAETAGSVRILVKLNMPFVPDGQLSPKQSEEQQRYISKMQDQLCTV
                     LVQHDVQGIKRFKYTPYMAMRVNAAALKVLISSSFVSSIEAESAMPPTER"
BASE COUNT          687 a          751 c          638 g          720 t
ORIGIN      
        1 cattcactgc ctgcacccgg taataataag ttgtgccgaa cgtcagcccc gtgtcgctat
       61 aactcgtcga attggcactc acagtagcga tctccgtata aggcccaccg gaaacgcctg
      121 cccggctgat gatgaactcc gactccgcgc cggaattatc cgtccagctc aaattgattt
      181 gtgtatagga aacagccgat gcgatcagac cgctgggtgc cccaagcatg gtcactgcgg
      241 atgcctcatt ggaatccgga gagtcccctt cagcgttata tgcactcaca cggtagtaat
      301 aggtctttcc ctcactgaga ccggtatccg aataggtaac cgttccggcg ccgacagtgg
      361 caacctggct gtatgtgccg cttgcacccg tttttctatc aaccttaaac cccgtctcgc
      421 ctgtagagtt gtccgtccag ctcagatcga tcccgctggt tgatactgaa gaaccggaaa
      481 ggcccgtcgg catattcaac gcgagattaa cattcagggc agccagaaca tttattctct
      541 ttatggaacc acccgccata tcccccgctt ttaaagtcac ggctacacct gtatcctgaa
      601 gtccctttaa tacatttgca acggaagccg tcggccattt ctgcttgatg atggcccagg
      661 cgcccgccac atgaggggct gccatggacg ttccatccca gctgtcccag cctccgcccg
      721 gaaccgaaga attgatggcg cttccgggcg caaagagaga cagcatgaca gggtgatagt
      781 tactatagtc cgcctcggca tccgcttttg tcgtcgcccc cacacttact gcagaagaaa
      841 cacaggcagg tgaactcaca ctgtttttat aaccttcgtt ccccgatgat atgacggtgg
      901 caatttccgc cgaacgtagg ttgtcaatga cggcctttgt agaagattcg gaagtatcgc
      961 aggcggtaaa atattgacca cctccgatgc ttatattgac ggctgcaatg ttataggtat
     1021 ttttcaatga gtaaacgtgt tccagggcaa gattctggtc agacgtgaag gaaagcgcac
     1081 aaggtgaagt aagaccgtaa ctgctgcagg atgctgcgtc agtgattttt gaaaatacct
     1141 gaatggcaat gatgttcgct tctttggcca caccatttat ggaagccgac ttgcccgcgg
     1201 caataccagc cacatgggtt ccatgcttac atccatcaat ttctgtaccg tttgctcccc
     1261 cgcactggat tcccgcgcct gctcctgtct gagactcatt gccatccgga caaaccgttt
     1321 ctgcgtgctg gccggcgtca gtggtggaaa agcaagcctc tgcaaccact ttaccggcag
     1381 gaaagaagct gtgagtttta tcaactccag aatcaagaac agccaccgtc catcctgatc
     1441 ctgtataacc cagtccccag gcttgatctg ccttgatcaa agggacactc tccgtcaata
     1501 tcgccggaac aagcttatct tattcaatcg aaaccacaag gggattggca atcaaagcat
     1561 ccagcgcatt ggaatccacg gccatagcag tgtacggaat atgtttaaat ttttttattc
     1621 ccctcacatt gtgtttggaa aattcttgct gcagccgggc ctgcgtactt gctattcggg
     1681 ccttttggcc agcaaccgct gtcggtcctg acaactgccc ctccggctga aaagacgtat
     1741 tcaatcttac gatgacccgc acgctgcccg ccttttgtac cttggcaagg agcgcaggag
     1801 atgctttctg gctgccctgc ccttcagccg ccatgatacc gccaaaaagc aagagaacaa
     1861 aaaatgtgaa tgcaaacagc cggcaaaacc gatgtgccca attggcttct gattccagat
     1921 ttctgtatgc cttgtctgcc atcctacccc tcctgaaaat cctatcattc acttatctga
     1981 caacgcctgt tttataaaat cacttttccc ggcctatctt tctgttggcg gcatggcact
     2041 ttccgcttca atcgatgata caaaggagct ggaaatcagc accttcaggg ctgctgcatt
     2101 cactctcata gccatgtagg gtgtatactt gaatctttta atgccttgga catcatgctg
     2161 aaccaatacc gtgcacagct gatcctgcat ttttgatatg tatctttgtt gttcttccga
     2221 ctgtttcggg gataattgac cgtcagggac aaacggcatg tttaatttta ccagaattcg
     2281 aacacttcct gcagtctccg ccacggtaag aagccggctg tagtctttga ctgaagatgt
     2341 actttcgttt tgtgaaacag gaggatttgc ctccaggttg tgcgcattca cgatttgaga
     2401 gcagcctgga agtatcatca agaagaggga tgcaagacat ggccagatct ttaaaaatct
     2461 ttttttcagc atatgaagtc tgtcccgatc aggtaatcag ttcagggatc tcccgccatg
     2521 atctttcatg gcgcaaaagc ggttctgctc aaggtgtagc ctgttattaa ttatacatcc
     2581 agtcgtggag aaaatgcaac agaacttctt gtaaatcgcc aatgtttgtc tgtaaatgct
     2641 cagcgagcaa cgataacttc caggaaaaaa agaatcatgt cggggaagcc atagcaaata
     2701 aatagaaata tcaggacgac tgcttctttt ctctctccct tatgaaacag cgcggatctt
     2761 ccgccaggta gtcgccactg tatgcatggg cccgcc
//
LOCUS       DAOPWB010000230         2796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_37390_length_2796_cov_4.445093, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000230 DAOPWB010000000
VERSION     DAOPWB010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37390_length_2796_cov_4.445093"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PKN92_13115"
     CDS             complement(<1..825)
                     /locus_tag="PKN92_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNZ49778.1"
                     /translation="MAEDTKAKDLAMDLAEDARESNWEYRSFTAEMFRGTFGWDLMHP
                     FPAQTEEDKKIGDDLLVKVKEVLETYVDPYETDRTGEYSREALDKLAEIGLFGMKIPK
                     KYGGLGLSISNYARVLGMVGSYCGSTVTYLSAHQSIGVPEPLITFGTEEQKEKYLPRL
                     AKGEISAFALTEPDVGSDPAKMTSWAEPSEDGTYYTLNGDKLWCTNGTDPKTGLIVVM
                     ARTPDKISKSGKPIPQISTFIVEMNWEGVEVVRRCQFMGLRGIANGALTFKNVKVPA"
     gene            complement(949..2520)
                     /locus_tag="PKN92_13120"
     CDS             complement(949..2520)
                     /locus_tag="PKN92_13120"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNZ49779.1"
                     /translation="MFWNKNKKDPESEKSFYSNLNPYAAKSARPQSSLVKRLTTKLRK
                     SNPPSGEAPLAEPSPVLQPGDADVSDQPVALNPDEESLLSSEEMTEDSLPGVLVMQGV
                     ASPAQMDEALRRQQENGVFFGDALADMGAVTATHLVGFLSKYCQIPHLSLLDYVVDKD
                     TANLLPAEVCWKYYVLAIDKMGRNLTVAMVNPLNEEARAEIARQCPTLRVKPILCSHK
                     DFETVATRIFGPRQDKSDKHWTRVSQNMMPTSTEGAPQTADTSPPPADPRPVIPAAPV
                     IPAQAQAPEMHSSEINFSINDSFVDHVFFDADNQVGQALSEFKSTPVTSKFKLSNTQD
                     RADEIVKSFSSEQDLVSQYTEMMMESMRNSYELLARKIPFFHGIEARDVTKFFSHGQV
                     KVYEFGQIVYEKGEFSNEMYIILNGCVELHAPGAEPIVLRRGDIFGETALRGGEVRSE
                     SARVIAHSSIMKVDFKSLTESLSQSVSTQLLANIVVILGQRLERVRRGQEITHYHWLD
                     ETASKNSANPSDEAD"
BASE COUNT          665 a          749 c          674 g          708 t
ORIGIN      
        1 cgcaggcact ttaacgtttt tgaacgtaag ggcgccgttc gcaattccgc gaagacccat
       61 aaactgacag cgacgaacca cttcaacgcc ttcccagttc atttcaacga taaaggtgga
      121 aatctgcgga ataggcttgc cgcttttgga gatcttgtcg ggagtgcgcg ccatgaccac
      181 gatcagccct gtcttgggat cggtgccgtt ggtgcaccac agcttgtcac cattcagcgt
      241 gtagtaagtg ccgtcttcgg aaggttccgc ccagctggtc attttggcag gatccgaacc
      301 gacatcgggc tccgtcaatg caaaagcaga aatttctccc ttggcaaggc ggggcagata
      361 tttttctttc tgctcttcgg taccgaaagt aatgagcggc tcaggcacac cgatgctctg
      421 gtgcgcggaa agataagtca ccgtgctgcc gcaatagctg cccaccatac ccagtacccg
      481 ggcatagttg gaaatggaaa gaccgagccc gccgtacttt ttggggatct tcatgccgaa
      541 cagaccgatc tccgctaatt tgtccagcgc ttccctgctg tactcaccgg tgcgatccgt
      601 ctcgtacgga tccacatagg tttccagtac ttctttgact ttcaccagaa ggtcatcacc
      661 gattttcttg tcttcctctg tttgcgccgg gaatgggtgc atcagatccc agccaaaggt
      721 tccccggaac atctctgctg taaagctgcg atattcccag tttgactcgc gcgcatcttc
      781 cgccagatcc attgccagat cttttgcttt tgtgtcttca gccatgatta cttcttcctt
      841 tgttctgaaa ttggtttcct tacctaccgt caagtatact ccattgtatc acggtttcaa
      901 ctatattacc agattttatt tgctgagtcg atccaaaaac agcggggctc agtcggcctc
      961 gtccgagggg ttggcactgt ttttggacgc tgtctcatcc aaccaatggt aatgtgtgat
     1021 ttcctgcccg cggcgaaccc gctcaagccg ctgtccgaga atcacaacaa tattcgcaag
     1081 aagttgtgtg gaaacactct gggaaagcga ttccgtcagt gatttaaagt ccaccttcat
     1141 aatgctggag tgggcaatga ctcttgccga ctcactgcgc acctctccgc cacgcaaggc
     1201 tgtttcacca aagatatcgc cgcggcgcag cacgatgggt tcagcacccg gggcatgcag
     1261 ctcaacacag ccattcaaaa taatatacat ttcattactg aattcccctt tttcatatac
     1321 tatctgcccg aactcgtata ccttgacctg gccatgggaa aagaacttgg tgacatcccg
     1381 tgcttctatc ccatggaaga aagggatttt ccgtgcaagc aattcatagg aattgcgcat
     1441 gctctccatc atcatttccg tgtactggct caccagatcc tgctcgctcg aaaacgactt
     1501 gacaatctcg tctgcccggt cctgcgtgtt ggagagtttg aatttgctgg ttaccggcgt
     1561 gcttttaaat tcggacagtg cctgccccac ctgattatcg gcatcaaaaa agacatgatc
     1621 cacgaagctg tcgtttatgc tgaagttaat ttcggagctg tgcatctcgg gcgcctgtgc
     1681 ctgagcaggg atgacaggtg ccgccggaat aacaggtctc ggatcggcgg gcggcggcga
     1741 tgtatccgct gtttgcggcg caccctcggt ggaggtgggc atcatgtttt gcgagacacg
     1801 tgtccagtgc ttgtccgact tatcctgtcg tggcccgaaa atacgggtag caacggtttc
     1861 aaagtcttta tgtgaacaga gaatcggttt gacacgcaaa gtcgggcatt ggcgtgctat
     1921 ttcagcacgc gcctcctcat tcagcgggtt caccatagca accgtaaggt tgcgtcccat
     1981 tttatcgatg gcaagcacgt aatacttcca gcagacttct gcgggaagca ggttcgccgt
     2041 atctttgtca acaacataat ccagcagact cagatgcgga atctggcagt acttggacag
     2101 aaaaccaacc agatgcgtag cggtgacagc gcccatatcc gccagcgcat ctccaaaaaa
     2161 tacgccgttt tcctgctgac gccgcaaggc ctcatccatc tgcgcgggag aagcaacgcc
     2221 ctgcatcaca agaactccgg gaagagaatc ctctgtcatt tcctctgaag ataacaggga
     2281 ttcctcatcc ggatttaaag ccaccggctg atcggaaacg tctgcatcgc caggctgaag
     2341 cacaggagag ggctcggcaa ggggagcctc accggaaggc gggttgcttt ttcttaattt
     2401 tgtggtcagc cgcttcacca gggaactctg tggccgggcg gacttcgccg cataaggatt
     2461 cagattggaa taaaaagact tttcagattc cgggtctttt ttatttttat tccagaacat
     2521 aagaacaacc ctttcgccac tttttcagaa cgccaaaatc tgatgaatgg cgctttcact
     2581 ccatacaaag cagttaacaa aataaacagg tatcttgttt ccttattaac aaaggtgccg
     2641 gtctgtacct gcaaaataga ccgcatttca ttaggcatag caacagttgc tgcagtcagc
     2701 acgcggaggc tgtccgcacc attcagttgc tcaatagccg acatatatta tagtaaaatc
     2761 agcgtaagaa cgcaatacaa ctgagaaaaa tgacgg
//
LOCUS       DAOPWB010000231         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_39110_length_2710_cov_5.749529, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000231 DAOPWB010000000
VERSION     DAOPWB010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39110_length_2710_cov_5.749529"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..2062)
                     /locus_tag="PKN92_13125"
     CDS             complement(8..2062)
                     /locus_tag="PKN92_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNZ49780.1"
                     /translation="MKISSTSRTLWLLPLFLFSAACSLFADERPDSITDQLSLSIKAA
                     AARVTPALVRIHVVEAYYREGREMKSESSGSGVVIDAQGHVVTNHHVAGHAKQMKCVF
                     ADKSEYAAELVGTDPLTDIAVIKLKGGENSSFNLAQWGDSSAVRVGDTVLAMGSPLAL
                     SQSVTRGIISNTEMTLPSWMSQMGGLTLDGENVGALVRWLAHDAQIFGGNSGGPLVNM
                     AGEIIGINEISLGLSGAIPGNLARSVAQELIASGSVRRAWVGLEVQPRLKSDSRSTGV
                     LVSGTIADSPARSSGLEPGDLLLSVGGEPVDIRFPVQLPDFNLMITHLPIGEAVAFEV
                     ERSGEKKILSVTPVEREIFEQKEYEEIQWGITVRNISFMMAKELKRKSTDGVLVTSVR
                     SGGPAGDAKPPISTNDVIVSIGGETVKNVRQFQEVTERITGNASEPVPTSTLLERKND
                     HLLTVVKVGIREPRDPGQEVRKAWLPIEYQVITRDIAGSLGDESLKGYRVSQVYKGGT
                     AEKAGLLVGDLILAVDDEPMTASSTEHYEELAALIRQYSIGDTVAVQVKRGNETLSLS
                     IELAASPKLPREMKKIEDEHFEFTARDITFFDRAAEQWAESQSGILVEQVKPGGWAAL
                     GSLTPGDLILEVNGTALSSVDIARKILGDLSEEKAPSIVFKVQRGIRTLFLELEPQWS
                     NS"
     gene            2327..>2710
                     /locus_tag="PKN92_13130"
     CDS             2327..>2710
                     /locus_tag="PKN92_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNZ49781.1"
                     /translation="MSDQLLHVVNPFTEKEEFTLPLLKPQEIDLVVQRAQNAFSVWRS
                     TSMGERIACCQRFADAVEKKRDEIARNLTRQMGKPLQQSLNEVSGMLGRMDYMMSIAE
                     KALADEWLPDVPGFKRYIRHEPLGVV"
BASE COUNT          616 a          761 c          689 g          644 t
ORIGIN      
        1 aggtcaatca actattgctc cactgaggct caagttcaag aaaaagtgtg cggatgccac
       61 gctggacttt gaaaactatg gaaggggctt tctcctcgga gaggtcgccc agtattttgc
      121 gtgctatgtc gacagaggat agcgccgtcc cattcacttc cagaatcagg tcgcccggag
      181 tgagactgcc gagagccgcc caaccgcccg gcttcacctg ttccacaagt atgcccgact
      241 ggctttccgc ccactgttct gccgcgcggt caaaaaaggt gatatcccgt gccgtaaact
      301 caaaatgctc atcctcaatc tttttcattt cccgtgggag tttcggcgat gccgccagtt
      361 ctattgaaag ggagagcgtt tcattaccgc gttttacctg cacagccact gtatcgccaa
      421 tggaatactg acggattaaa gcagccaatt cttcgtagtg ctctgttgat gaggcggtca
      481 taggctcatc atctacggca agaatcaggt cgcccaccag caggcccgct ttttccgcag
      541 taccgccttt gtacacctga ctcacacggt agcctttcaa ggattcatcg ccaagagaac
      601 cggcaatatc ccgggtgatc acctgatatt caatcggcag ccaggctttt cgcacttcct
      661 gacccgggtc acggggctct cgaatcccta cttttaccac agtcaacagg tggtcatttt
      721 tgcgttccag cagagtcgaa gtcggtacgg gctcagacgc attcccggtg atccgttctg
      781 ttacctcctg aaactgccgg acatttttca ccgtctcacc accgatggag acaataacat
      841 cgttggtgct gatcggtggc ttcgcatcac ccgccgggcc gcccgagcgc accgacgtta
      901 ccagcacccc gtccgtactt ttccgtttga gttcctttgc catcatgaag gaaatattgc
      961 gtaccgtgat gccccactga atttcctcat attccttttg ctcaaaaatt tctcgttcta
     1021 ccggcgttac agacagaatc ttcttttcac ccgaccgttc cacttcaaaa gcgacggctt
     1081 cgccaatcgg gagatgggtg atcataaggt taaaatcagg aagctgcacc ggaaagcgga
     1141 tgtctacagg ctccccgcct accgataaaa gaagatcgcc cggttcaagc ccggatgacc
     1201 gagcaggtga atcggcaatg gttccactga caagaacgcc tgttgaacgg ctgtctgatt
     1261 tgagccgggg ctgaacttcc agccctaccc aggcacggcg gacagagccg gaggcaatca
     1321 gttcctgcgc caccgaccgt gccagattgc ccgggatggc accgctcaag cccaaactga
     1381 tctcgttaat ccctatgatt tcacctgcca tattcacaag cggaccaccg gaattgccac
     1441 cgaaaatctg tgcgtcgtgg gctagccagc gcaccagtgc gcccacgttt tcgccgtcca
     1501 gagtcagccc gcccatctgg ctcatccagg agggcagggt catttctgta ttgctgatga
     1561 taccccgcgt cactgactgg gacagcgcca gcggactgcc catggcaagc acggtgtcgc
     1621 ctacacgtac ggcgctggaa tcaccccatt gcgcaagatt gaaactgctg ttttcgccgc
     1681 ctttcagttt aatcaccgca atatctgtca atggatccgt gcctaccagt tctgcggcat
     1741 actcgctttt atcggcgaag acgcatttca tctgtttggc atgccccgca acatgatggt
     1801 tggtcactac atgcccctgt gcatcgatga ccacgccact gcccgacgac tctgatttca
     1861 tttcacggcc ttccctgtag taggcctcga cgacatgaat ccgaaccaga gcaggggtca
     1921 cccgggccgc agccgctttg atggacaacg ataactgatc ggtgattgaa tctggtcttt
     1981 catcggcgaa cagagagcaa gcagcggaga aaagaaaaag gggaagcagc cagagagtgc
     2041 ggctggtgct ggatattttc atgatgatgt cccttcaaga acgattgagt ttccctggga
     2101 aaagttgccg gtacgccccg gcgcgaagag cctcatttta gcacagtaca taatgattgt
     2161 cgtatttccg gggcgactgg cgtgaagagg cagaaaaaaa acggtctttt cgggtgacag
     2221 gatcaagagc tgccttcacc cttggatccg tcaatggctt cacttttgga ttttttttaa
     2281 tactttcgta taatcaacgc ttccttaact taacgggagt gttgccatgt ccgatcagct
     2341 tcttcatgtt gtgaatcctt ttacggaaaa agaagaattt acgctgccgc tgctgaagcc
     2401 gcaggaaatt gacctggttg tgcaaagagc acagaacgcc ttttctgtgt ggcgcagcac
     2461 ttccatggga gagcgtatcg cctgctgtca gcgatttgct gatgctgtgg aaaaaaagcg
     2521 cgatgagatc gcccgcaatc tgacccgcca gatgggcaag cccctgcagc agtcgctcaa
     2581 tgaagtctca ggcatgctgg ggcgtatgga ttatatgatg agtattgccg agaaggcact
     2641 tgcagatgag tggcttcccg atgtgcccgg attcaaacgc tatatccgtc acgaaccgct
     2701 gggggttgtg
//
LOCUS       DAOPWB010000232         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_39247_length_2704_cov_4.966780, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000232 DAOPWB010000000
VERSION     DAOPWB010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39247_length_2704_cov_4.966780"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PKN92_13135"
     CDS             complement(<1..625)
                     /locus_tag="PKN92_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNZ49782.1"
                     /translation="MFLDMFLKVAGAASIAALLFVVYLARQVLSKPQGTERMAFLSTQ
                     IQKGAAAFLKREYTWVSGFVLVIMLFFVVLGMVKPDLALNWKTAVSFLAGSVASACAG
                     VVGMWIATRANARTAAAAEHSGVKGALDIAISGGAVMGMAVVGISLLGLVIIYKIFDG
                     NPTIVNGYAMGASLVALFGRSGGGIFTKGADMGADLVGKVEAGIPEDD"
     gene            complement(714..1433)
                     /gene="rnc"
                     /locus_tag="PKN92_13140"
     CDS             complement(714..1433)
                     /gene="rnc"
                     /locus_tag="PKN92_13140"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNZ49783.1"
                     /translation="MQQDANRIRALRAFAEKIAVPLHDPSWLDRALTHSSSLPEGLAT
                     SGKDFESLEFLGDAVLELAISHRLFELIPDKTPGEYTRLRASIVSRVNVARIASELSL
                     SSMIILGKGEEQSGGRKRSSLQGDCLEAVIGAVYLDAGWEAARDFVFRIFSLDLAALQ
                     RSPHCWDYKSLLQIYCQKSHIALPLFKVVNCSGPDHRKHFVVAVYVRDKLQGRGSGWS
                     KKEAEQDAARKALLILEKPES"
     gene            complement(1481..1726)
                     /gene="acpP"
                     /locus_tag="PKN92_13145"
     CDS             complement(1481..1726)
                     /gene="acpP"
                     /locus_tag="PKN92_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNZ49784.1"
                     /translation="MSDDLALKIKQVIAERCNRSLEQVTDDARFSEDLGADSLDITEL
                     MMALEEEFNITIEDDAAHISTVGDAIDYIKKSQSKKD"
BASE COUNT          653 a          777 c          649 g          625 t
ORIGIN      
        1 gatcgtcttc aggaataccc gcttccactt tacccaccag gtcagcgccc atgtcggcgc
       61 ccttggtgaa gatgccgccg ccggaacggc cgaaaagagc aaccagagag gcgcccatgg
      121 cataaccgtt tacaatagtc ggattgccgt caaaaatttt gtagataatg accagaccga
      181 gcagggaaat gccgacgacc gccatgccca taacagcacc gccgctgatg gcgatgtcca
      241 gggccccttt cactccggag tgctcggcgg ctgctgctgt gcgtgcattg gcgcgggtgg
      301 caatccacat gccgaccaca ccggcgcagg cgctcgccac agatccggca aggaaagaaa
      361 cagctgtttt ccagttcagc gcaaggtcag gctttaccat gccgaggacg acaaagaaca
      421 gcattattac cagaacaaac ccgctgaccc aggtgtattc ccgcttcaga aaagcggccg
      481 cgcctttctg gatctgggtg gacaggaagg ccatccgttc ggtgccctgc ggctttgaga
      541 gcacctgccg cgcaagatat acgacgaaga gcagcgcggc aattgatgcg gctcccgcca
      601 ccttcaggaa catgtcaagg aacataataa tgtgtttcct tcctattggt tatggaattc
      661 ctgaacgagg tcagcccccg tatacgccat cgtataaagg gagttgctca tcttcaggat
      721 tccggctttt ccagaatcag aagcgctttc cgcgccgcgt cctgctccgc ctctttttta
      781 ctccagccac tgccacgacc ctgcaatttg tcgcggacat acaccgctac cacaaaatgt
      841 tttcggtgat ccggaccact gcaatttacc actttgaaga ggggtaaagc aatatgtgat
      901 ttctgacaat aaatctgtag cagcgattta tagtcccaac agtgcggtga ccgctgcaat
      961 gccgcaagat ctaacgaaaa aattcgaaat acaaagtcgc gggcggcttc ccacccggca
     1021 tccagataaa ccgcaccgat cactgcttcc aggcagtcgc cctgaagact gctgcgcttt
     1081 ctgccgccgc tctgttcttc acctttccca agaattatca ttgacgaaag ggaaagttcg
     1141 gacgcaatgc gggctacatt gacccggctc actatagaag cgcgcaaacg ggtatattcg
     1201 ccgggtgttt tgtccggaat cagttcaaaa agccggtgag aaatggcaag ttcaagaact
     1261 gcatcaccta aaaattccag agattcaaaa tctttgccgc ttgttgccag accctcaggc
     1321 aggctggaag aatgcgtcag ggcacggtca agccacgaag gatcatggag cggtacagcg
     1381 atcttttccg caaaagcgcg cagagcgcgg attcggttgg catcttgctg catggtgctt
     1441 gctgaagggt gctgcggctg atgccggtca gacaccgctt ttagtccttc ttactttggc
     1501 ttttcttaat atagtcgatg gcgtcaccaa cagtgctgat gtgtgccgca tcgtcttcaa
     1561 tggtgatatt aaactcctct tccagagcca tcatcagttc cgtgatatcc agagaatcgg
     1621 caccaagatc ttcgctgaag cgggcatcat ccgttacctg ctccagagaa cggttgcatc
     1681 tttccgctat cacctgcttg attttcaggg caagatcatc actcatggtt actctccttg
     1741 ttgctgtggc ttttcagacc gcgacggggt acagggaaaa ttcccggaaa cagacccgcc
     1801 acaccgctgg cagcacagat accggtttcg gacaaggccg cacttccaat caggatggct
     1861 ccagcagatt aactgcaaaa actcctgacc cgaaagaagg caggctgact gtcaacagta
     1921 cttcctcggt tgtctgcatg tgaaaagaaa catttctgac tgtttagttc agcggactgc
     1981 tattattatg ccataaacaa caccgtataa ccaaaatgtt tgtatgctaa cagtccgcag
     2041 tcggaaatgc agggagaaac tacgcaaatt ccggcgcttc aggcattttc tgaatcgtcc
     2101 tgcaccgtct gttgttgttg cgtggtaaat acagggaaag tctgtgcaaa aaaacagcaa
     2161 aaaataactt attccctagt ctgtacctgc attactctgc attgcccccc gtcgtcattc
     2221 ctgcctcccc cctccgtcat tcctgcctcc ccccgtcatt cctgcactcc ccgtcattcc
     2281 tgcctccccc cgtcattcct gctcctccct ccgtcattcc tgcctccctc cgtcattcct
     2341 gcctccctcc gtcattcctg cctctccccg tcattcctgc gaaggcagga atcttaatca
     2401 gggtatggac gtcttgactt agacatcgcc ctgatccaca ccaaagactt caagacatac
     2461 aaacacaaaa taagccttaa tttaaaagac ttctccgcgt agttgacggc aacagcatga
     2521 cccttataaa taacatctcg cgttaagatt cctgcctgcg caggaatgac gccgggggag
     2581 gcagccatga cgcctgaggg tggacgtaac ggcggaaggg agaaatgaca gcgtcgtgtg
     2641 taggactggt ggaggaggta tgtctcaggg catcactccg ctgtgaatcc gagtgactgt
     2701 cttc
//
LOCUS       DAOPWB010000233         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_39399_length_2696_cov_3.947368, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000233 DAOPWB010000000
VERSION     DAOPWB010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39399_length_2696_cov_3.947368"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PKN92_13150"
     CDS             <1..1010
                     /locus_tag="PKN92_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNZ49785.1"
                     /translation="MQVLIDAPRARTAGLTPMIIAQTVDAALRGVRMPFLKQDGREIP
                     VWAQFREEDRRSQANLENIALQGLNQQLTPLSRLTNFSKAPSPRSIRRLDGKNVLSIS
                     AKTDSNNLMEVKAELKAMLDNIDLPPMYSYSFGDEFNELEDNLLNFSLSMTMAVILVY
                     LVMCALFESFILPFTIMTTVPLAMIGAIWLLYLTNTSFDSISLIGCILMAGVIVNNGI
                     VIVDHMRNTCKEFAVRDDGIVKAGKDRFKPVLMTAITTILGLVPVAIATTGGASTFAG
                     LGRTLIGGLSVGTVLTLFIVPIFYGLLDDLSIWLRNFMASVQMLPLNAETEPERPAAA
                     K"
     regulatory      1068..1187
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1297..2412
                     /gene="ribD"
                     /locus_tag="PKN92_13155"
     CDS             1297..2412
                     /gene="ribD"
                     /locus_tag="PKN92_13155"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNZ49786.1"
                     /translation="MLADHVYMNRALTIAKRGRGWTSPNPMVGCVIVRDGVVIAEGWH
                     QRVGMPHAEAMACEQCPPEALQGSVVYVTLEPCAHYGRTAPCTDLFLTHRPSRIVIAM
                     EDPNPHVKGGGIEILRSAGITVEVGLLEKEAARLNEIFIKYSKTGMPWVTAKCAMSLD
                     GKIATHTGHSRWITGMTARLATHELRHAHDAILVGSRTVHYDRPRLTTRLMYKEGRNP
                     VRIILDAEGILEGNRDAFQDTPDVPVWVAGKKNGDYPFADEVIDLPVNASGMDMPALM
                     ASLGARGVTSVLIEGGGETLASAFFSGVVDKLCFFVAPKIIGGRDAVTPVEGKGIDYM
                     NEALHFTDLTVQTYGEDVCLEAYVTHETSGRQDAAGV"
     gene            2416..>2696
                     /locus_tag="PKN92_13160"
     CDS             2416..>2696
                     /locus_tag="PKN92_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNZ49787.1"
                     /translation="MFTGLIEDIGVVSRRSGSDLAILSKKLLDDLKEGDSVAVNGACL
                     TVAALTADGFVVQVSQESYARTTLGALKPGHAVNLERAMRADGRFGGHFV"
BASE COUNT          627 a          659 c          731 g          679 t
ORIGIN      
        1 agatgcaggt actcattgat gcacccagag cacgcaccgc cggtctgacg ccgatgatca
       61 tcgcgcagac ggtcgacgct gctctgcgtg gtgtgcgcat gcccttttta aaacaggacg
      121 gcagagaaat tcctgtatgg gcgcagtttc gcgaagaaga ccgccgctcc caggccaatt
      181 tggaaaatat cgctttgcag gggctgaacc agcagctgac ccctttgagc cggctgacca
      241 atttttccaa agcaccgtcg ccccgttcca ttcgaaggct ggacggaaaa aatgtgctca
      301 gtatttcggc aaagacagac agcaacaacc tgatggaagt gaaggcggag ctgaaggcga
      361 tgctggataa tattgatctg ccacccatgt actcctattc ttttggggat gagtttaatg
      421 aacttgagga taacctcctc aacttttcgc tttccatgac catggccgtc attctggttt
      481 atctggtgat gtgcgctctc ttcgaatctt ttattcttcc cttcaccatt atgaccactg
      541 ttcctcttgc catgattggt gccatttggc tgctgtatct gaccaatact tccttcgatt
      601 ccatctcgct gattggatgt attctcatgg caggggtgat tgtgaacaac ggcattgtca
      661 tcgtggatca catgcgcaat acctgtaaag aattcgctgt tcgtgatgac ggcattgtaa
      721 aagcgggtaa agatcgtttt aaacctgtgc tgatgacagc gatcacgacc attctcggtc
      781 tggttcctgt cgctatcgcc acaacaggcg gtgcgtccac ctttgcaggc ctcggcagga
      841 cactgattgg tggcttgtcc gtcgggacag tgctcacact cttcattgtt cccatatttt
      901 acggactgct ggacgatttg agtatctggc tgcggaactt catggcaagc gttcaaatgc
      961 ttccgttaaa cgcagaaact gaacccgaaa ggccggcagc tgcgaaataa tttctttgct
     1021 ttatttgatt tgattcgctc atacacttta caatagatct aacaatagtc agccttcggg
     1081 gcagggtgaa attcccgacc ggcggtgaaa gcccgcgagc gtgaaaacgc tgactccggt
     1141 gaaaatccgg ggccgacggt acagtccgga tgaaagaagt cgctgaacgc cgttaccgga
     1201 aaagaaatta ctgctgatcc ggaatacgga cggtatccgg atctgaccgg atgcaatccc
     1261 cgaaggaatg aaataaatcc tttcggggta ttttttatgc ttgcagatca tgtatatatg
     1321 aatcgcgccc ttacgattgc caaacgtggt cgtggttgga ccagtcccaa cccgatggtt
     1381 ggatgtgtta ttgtccgcga cggcgttgtc atcgctgaag ggtggcacca gcgggtgggt
     1441 atgcctcatg cagaagccat ggcgtgtgag caatgcccgc cggaagcact gcagggttct
     1501 gttgtatatg tcaccctgga gccctgtgca cattacggcc gcaccgctcc ctgcactgat
     1561 ctttttctca ctcaccgtcc gtcgcgcatt gtgattgcca tggaagaccc caacccgcat
     1621 gtgaagggcg ggggcataga aattctccga tccgcaggaa ttactgtcga agtcggactg
     1681 cttgaaaaag aagcagcccg gctcaatgag atttttatta aatattcgaa gaccggcatg
     1741 ccctgggtga ccgccaaatg cgccatgagt cttgacggga aaattgcgac ccatacgggg
     1801 cattcccgct ggattaccgg gatgactgca cggctggcaa cccatgaact gcgacatgct
     1861 catgatgcta ttctggttgg cagcagaacc gtgcattatg acaggccccg gctgacaaca
     1921 aggcttatgt acaaagaggg aagaaatccc gtacgcatta ttcttgatgc ggagggtatt
     1981 cttgaaggaa acagagacgc ctttcaggat acgcccgatg ttccagtatg ggtggcaggg
     2041 aaaaaaaacg gagattatcc ctttgctgat gaagtgattg atttgcctgt aaatgcttcc
     2101 ggcatggata tgcctgcgct catggcttct ttaggagccc gcggggtcac ttctgtattg
     2161 atagaaggcg gcggagaaac gcttgcctcc gcctttttct ccggcgttgt tgataaactg
     2221 tgttttttcg tcgcaccaaa gattattggc ggacgcgatg cagttacccc tgtggaaggc
     2281 aagggaatcg attacatgaa cgaggcactc catttcaccg atttaacggt gcaaacttac
     2341 ggcgaagatg tctgtctgga ggcgtatgtt acccatgaaa cttccggacg gcaagacgcg
     2401 gcgggagtat aggcggtgtt tactggatta atagaagaca taggtgttgt ttccaggcgc
     2461 agcggcagtg atcttgccat cctgtcaaag aaattactgg atgatctgaa agaaggtgac
     2521 agtgtcgccg taaacggtgc ctgtctgacg gtggctgctc ttactgccga tggttttgtt
     2581 gttcaggtgt ctcaggaaag ctacgcacgt accacgctgg gcgcactgaa accggggcat
     2641 gcagtcaatc tcgagcgtgc catgcgggca gacggtcgtt tcggagggca ttttgt
//
LOCUS       DAOPWB010000234         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_40437_length_2646_cov_4.281359, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000234 DAOPWB010000000
VERSION     DAOPWB010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40437_length_2646_cov_4.281359"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKN92_13165"
     CDS             complement(<1..443)
                     /locus_tag="PKN92_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HNZ49788.1"
                     /translation="MAKDGTARGGARPGTGPKPKPLVEKISDGKAGGAMVLPEPAEFS
                     GVDVPPVKEFLKSEQKSGKDLCAEEVFNDTYRWLVERGCEKLVTIPQIEQYAMSVSRW
                     IQCEQCISEYGFLAKHPTTGNAIASPYVAMSRDYMKQINAAWYQI"
     gene            complement(436..597)
                     /locus_tag="PKN92_13170"
     CDS             complement(436..597)
                     /locus_tag="PKN92_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49789.1"
                     /translation="MRDRKKQRALRKAQREAEANKNINWHKNAEGYSDPTFYAAMKNA
                     EREGKKKNG"
     gene            complement(711..1058)
                     /locus_tag="PKN92_13175"
     CDS             complement(711..1058)
                     /locus_tag="PKN92_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNZ49790.1"
                     /translation="MPRKPKKPCAYPGCPNLTENRYCEEHAAKANREYDLYVRAPDKH
                     KKYGRAWKRIRDSYAREHPLCERCLEEGRVSLMDEVHHIVPVSQGGTHARSNLKSLCR
                     SCHQRTHQELGDR"
     gene            complement(1238..1699)
                     /locus_tag="PKN92_13180"
     CDS             complement(1238..1699)
                     /locus_tag="PKN92_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49791.1"
                     /translation="MSIMWQYLDKTAATEKALSDYGAMKDIIDNTDEKINTVHERMSS
                     VGSPVLSDMPRGPHNPQATEDRYIKGLDEIDVLKERYRQAVEYMEWMKPALASLTEDE
                     RACLETYYWVSYRERGGAIRAIMDYFGIEQSSAYNKKNRALAKLSLLLYGK"
     gene            complement(1782..2039)
                     /locus_tag="PKN92_13185"
     CDS             complement(1782..2039)
                     /locus_tag="PKN92_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49792.1"
                     /translation="MKKRVNDGLDPYQALANAVVQNAAEDYRAALKRLNKDPENVIAK
                     AEVQELKRFFRSQWYEVLTEVDGEYLINMLEKEYPDVDTGN"
     gene            complement(2036..>2646)
                     /locus_tag="PKN92_13190"
     CDS             complement(2036..>2646)
                     /locus_tag="PKN92_13190"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNZ49793.1"
                     /translation="ELDRYMALKSNMILPNEEITAANAATLCGKLVQMANGAIYNDNG
                     KSVDFHDRKLDALEDMVEAANGKPILVAYWYQHDLDRISNRLSEFGVKFDRINTSDSI
                     ERWNRGEIPVGLIHPASAGHGLNLQEGCSTIIWFGMTWSLELYQQTNGRLWRQGQKDK
                     TVVIQHIVCENTIDGEILRRLEKKDMTQEALIAAVKAEVRRR"
BASE COUNT          525 a          727 c          582 g          812 t
ORIGIN      
        1 taaatctgat accaggcggc attgatctgc ttcatgtagt cccggctcat cgctacataa
       61 ggactggcga tggcatttcc cgtcgtgggg tgctttgcca aaaatccgta ttcgctgatg
      121 cactgttcac actgtatcca tcgggatacg ctcattgcgt actgctcgat ctgcggaatg
      181 gttaccagct tctcacagcc acgctcgaca agccatctgt atgtgtcgtt gaatacttcc
      241 tccgcacata aatccttgcc gctcttttgt tcagatttca gaaactcctt tacaggaggg
      301 acgtctacac cgctgaactc tgccggttca ggcagtacca ttgcgccacc cgcttttccg
      361 tcactgattt tctcgaccag aggtttcggc ttcggccctg ttccgggtct tgcaccacct
      421 ctggcagtac catctttagc cattcttttt ctttccctct ctttccgcat ttttcatggc
      481 cgcatagaat gtggggtcag aataaccctc cgcatttttg tgccagttga tgtttttatt
      541 tgcctctgct tcacgctgtg ccttacgaag cgcacgttgt tttttcctgt ctctcataga
      601 tcctcctgaa tccgggggtt aataccccgt ttgaatagca atttttgcgt aaatgaccca
      661 gcgccggtct cctgcgggtc ccttggtaga gatgaagacc gccccttggg tcatcggtcg
      721 cccagctcct ggtgtgtccg ttggtggcac gagcggcaca ggctcttgag gttgctccgg
      781 gcgtgtgtgc ctccctgact gaccggcacg atgtgatgca cctcatccat cagcgagacc
      841 ctgccttcct caaggcaacg ctcacacagc gggtgctccc ttgcgtagct gtcacggatg
      901 cgcttccagg ctctgccgta tttcttgtgc ttatccggtg ccctcacata gaggtcgtac
      961 tcacggtttg ctttggcagc atgttcctcg cagtaacggt tctctgtcag gttgggacag
     1021 cctgggtaag cacatggctt cttcggtttt cttggcacgt tgttcatctc ctttcttccg
     1081 ggcattaaaa aaggccacga taaacgcagc cataaaaaag gtatgaaaaa agcccgaagg
     1141 atttctcccc cgagccattt ttccacgata ccattgtatc aggtttctgc cgtataagct
     1201 tccacgattt tactcatttt atttttcttt tcacttttta ttttccgtac agaagcaggc
     1261 tgagtttcgc aagagctctg ttctttttgt tgtaggcaga ggactgttcg atgccgaagt
     1321 aatccatgat agctctgatc gcaccgcccc tttcacggta agaaacccag taataggttt
     1381 caaggcaggc gcgttcatcc tcggtcaggg aagcaagggc aggtttcatc cattccatgt
     1441 attccactgc ctgacggtat ctttccttca gcacatcaat ttcatccagt cctttgatgt
     1501 agcggtcttc cgttgcctgc gggttatgcg ggcctcttgg catatccgat agcaccggac
     1561 ttccaacgga actcatgcgc tcatggacgg tgttgatttt ttcatcggtg ttatcgatga
     1621 tgtctttcat tgcgccgtaa tcactcagcg ccttttctgt tgctgcagtt ttatccaggt
     1681 actgccacat aatactcata atattcgcac ctccggtttt tatttccctc ggattggcac
     1741 gtttttacta tgattgtctt aggttgtctt ttcttttgtc attagttgcc ggtatcgaca
     1801 tcggggtact ctttttccag catattgata aggtactcac catcgacctc tgtcaggact
     1861 tcataccatt gggaacgaaa gaatcttttt aattcctgca cttccgcttt cgcaatgaca
     1921 ttctccgggt ctttattcag acgtttcaga gccgcccggt aatcttctgc ggcattctga
     1981 acgacagcat tggccagtgc ctgataagga tccagtccat catttactct tttcttcatc
     2041 ggcgacgcac ctccgcttta acggctgcaa tcagcgcttc ctgggtcatg tcttttttct
     2101 ccagccttct taatatttct ccgtcaatgg tgttctcgca gacgatgtgc tgaatgacta
     2161 cggttttatc tttctgaccc tgacgccaca gtcttccgtt tgtctgctga tacaactcaa
     2221 ggctccacgt cattccgaac cagataatgg ttgagcaacc ttcctgcagg ttcaaaccgt
     2281 gaccggcgct tgccggatga atcagaccga ccgggatttc tccacggttc cacctttcaa
     2341 tactgtcgct ggtatttatg cggtcgaact tgaccccaaa ttcagaaaga cggttgctga
     2401 ttctatccag gtcatgctga taccaatagg ccaccagaat cggcttgcca tttgcagctt
     2461 caaccatatc ctccagcgca tcgagctttc ggtcatggaa atcgaccgac ttaccgttgt
     2521 cattatagat tgcaccgttt gccatctgta caagctttcc gcaaagtgta gcggcattgg
     2581 cggcagtgat ttcttcattc ggaagaatca tattcgattt caaagccata taccgatcca
     2641 gctctt
//
LOCUS       DAOPWB010000235         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_40626_length_2637_cov_4.387684, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000235 DAOPWB010000000
VERSION     DAOPWB010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40626_length_2637_cov_4.387684"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PKN92_13195"
     CDS             complement(<1..1086)
                     /locus_tag="PKN92_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49794.1"
                     /translation="MVKWQIIMGILITCGLASANTLDITPFLGENWYGLYFNGEKVGY
                     LVKRTSLNDHKDIEVLEDAHFRVTMSGARQGMSITTTRTYAAAGDLISIDMEMQDPAQ
                     ASTFHARVDGDSMRVKSLLGGTAREETFPRPQESLTDALRDAVWVRSRPEPGAVINFS
                     VFEPMYQKEVMGMSRITGVEERILNGVPTRIYEIQSVLDLMDIESTSYVAEDGTTVED
                     VVSGVFTMRLEPEDSAKDVEYAVDTMVSNAVLLPERIADPRTRRWLRLWLKGPLREAH
                     LFNDVRQHMTLLDDKVDFYAERTDLSTLVIPQLPVEDPELLRHTKPSAYIQSDDAKLV
                     ARAREIVGDEKDILKASQKICSWVHGNM"
     gene            complement(1154..2188)
                     /locus_tag="PKN92_13200"
     CDS             complement(1154..2188)
                     /locus_tag="PKN92_13200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNZ49795.1"
                     /translation="MVQTGDYRHGIFFFLLCVCFVFPGLTGCGRMPTGAREALEDHRT
                     SRSAFVLALAGDVLLSPNCSRRPLAELECDNCGYGDLMRPARSFLKGADAAFCNLESP
                     LSMRGTAVPGKLWNFRAAPHAVKALTESGFNVVSVANNHILDYGSDAFADTLSHLERA
                     GIRYTGVSADGEQQNSVVIEAKGVRIAFLAYANVYPADYYHVPLRPVRATRERVRRDI
                     AQAKEQADHVIISIHWGMEYKAEPDPEQISLAHFMIDEGASVVAGHHAHILQDPEYYR
                     GGVIFYSLGNFLFMQGRLRTPETRVYALFLNKDAIVRVSYIPFDLKVNPWRMVYTPFS
                     RRAWRFPEAH"
     gene            2394..>2637
                     /locus_tag="PKN92_13205"
     CDS             2394..>2637
                     /locus_tag="PKN92_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium symporter"
                     /protein_id="HNZ49796.1"
                     /translation="MMQKRNHDGWATRFGLIMAMAGNAVGLGNFLRFPGKAAPFGGAF
                     MIPYFLVLVLVGIPLLWMEWTIGRIGGRYNHGSTPGM"
BASE COUNT          645 a          735 c          676 g          581 t
ORIGIN      
        1 catattgcca tgtacccagg agcatatttt ttgggatgcc ttcagaatgt ccttttcatc
       61 accgacaatt tcccgcgccc gggcaaccag tttcgcgtca tcgctctgga tgtaggcaga
      121 cggtttcgta tggcgcagca actccggatc ttcaacagga agctgcggaa tcacgagggt
      181 ggacagatcc gtgcgctccg cataaaaatc aactttgtcg tcaagaagcg tcatatgctg
      241 gcgtacgtca ttgaacagat gcgcttcacg cagaggccct ttgagccaga gccgcagcca
      301 gcgccgggta cgcggatcgg caatgcgctc aggcagaagc acggcattgg acaccatggt
      361 atccacggca tattccacgt ctttagcgga atcttccggt tccagccgca tggtaaagac
      421 accggacaca acgtcttcca ctgtcgtgcc gtcttcagca acataagaag tcgactctat
      481 atccatcaga tcgaggacac tctgaatttc ataaatgcgg gtaggaacac cgttgagtat
      541 tctttcttca acgcctgtaa tccggctcat gcccatcact tctttctggt acatgggctc
      601 aaaaacagag aaattgataa cggcgcccgg ctccggccgg ctgcgcaccc agactgcatc
      661 gcgcagtgcg tctgtaagcg attcctgcgg ccgcggaaaa gtctcttccc gggctgtacc
      721 gcccaaaaga ctttttaccc gcatggagtc accatcaacc cgggcatgaa aggtcgaagc
      781 ctgagcgggg tcctgcattt ccatatcaat actgatcaga tctccggcag cagcataggt
      841 gcgcgtggtg gtgatgctca taccctgccg ggcaccggac atggtcaccc ggaaatgcgc
      901 atcttccagc acttcaatgt ctttgtggtc gttcagcgag gtgcgtttta ccaggtatcc
      961 gactttttcc ccgttaaagt aaaggccgta ccagttctca cccagaaaag gagtgatatc
     1021 cagtgtgttt gccgatgcca acccgcacgt tatcagaatg cccatgatga tctgccactt
     1081 aaccatgaat cgtactctcc tcgatcttcc cattttaacg tattccctcc ccgtttatat
     1141 aacggagacc cctttagtgt gcttcaggaa aacgccaggc acggcggctg aagggcgtgt
     1201 aaaccattct ccagggattc actttcaggt caaaaggtat ataactgacc cttacaatcg
     1261 catctttatt caaaaagagt gcataaacgc gtgtttccgg ggtgcgaagc cggccctgca
     1321 taaaaagaaa attgccgaga ctgtaaaaga tcacaccgcc ccgataatat tccgggtcct
     1381 gaagtatgtg agcatgatgc ccggcaacca ccgatgcacc ctcgtctatc ataaaatgtg
     1441 ccagagatat ctgctccgga tccggttcag ctttatattc catcccccag tgtattgaga
     1501 taatcacatg atccgcctgc tcctttgcct gcgcaatatc ccggcggacc cgctcacggg
     1561 tagcacgaac aggacgcagt ggcacatggt aatagtctgc cgggtagaca ttggcatatg
     1621 cgaggaaggc gatgcgtacc cctttcgcct caatcaccac tgaattctgc tgctccccgt
     1681 cagcagatac gcctgtgtac cgtatgcctg ctctttccag atgagacaga gtgtccgcga
     1741 aagcatcgct gccgtagtcg agaatatgat tgttggcaac actgaccacg ttgaaaccgc
     1801 tctccgtcaa tgcctttacg gcatgcggtg ccgcccggaa gttccagagt ttgccgggaa
     1861 cggcagtgcc acgcatagaa agaggggact ccaggttgca gaaagcggca tccgcccctt
     1921 ttaaaaaaga gcgtgccggg cgcatcaggt ctccgtagcc gcagttgtca cattccagtt
     1981 ccgccagcgg gcgacggctg cagttgggtg aaagcagcac atcccccgca agagcaagga
     2041 caaaagccga gcgggaagtg cgatgatctt ccagtgcctc ccttgcgccg gtcggcatgc
     2101 ggccacagcc tgtgagcccc ggaaacacaa agcacacgca cagcaggaag aaaaagatgc
     2161 catgacgata atcacccgtt tgaaccatgc cttcactata atctttggta caggcaaatt
     2221 caaaaatacg gaaaaccagc cctgactcaa caggtataag ggcctttcgg aaggggcggc
     2281 acgcaaagga ataaagttga acaggactac cctgccgttt atactttaaa attcgaagtc
     2341 gcaagtagaa agttcatgca caacagatat atgcgaaaat atgctggaga cggatgatgc
     2401 aaaaacgcaa ccatgacggg tgggcaaccc gttttggctt gatcatggcg atggctggaa
     2461 acgcagtggg gctgggaaac ttcctgcggt tcccgggcaa ggcggctccc ttcggcggcg
     2521 ccttcatgat tccctatttt ctcgtgctgg tgctggtcgg gatcccgctt ctctggatgg
     2581 aatggacaat aggccgcatt ggcggccgtt acaaccatgg cagcacgccg gggatgc
//
LOCUS       DAOPWB010000236         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_41793_length_2587_cov_4.942733, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000236 DAOPWB010000000
VERSION     DAOPWB010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41793_length_2587_cov_4.942733"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..933
                     /locus_tag="PKN92_13210"
     CDS             178..933
                     /locus_tag="PKN92_13210"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNZ49797.1"
                     /translation="MSVRLIVSDVDGCISPEESCAWDGELFHTFAERCRRASSGESTL
                     APLTLCTGRPQPYVEVLMKLLDICFPAICESGAVIYRLDSNLSSFASSITTEMIQGLH
                     RVRDYITAEILPAVPGLLYQFGKEAQMSLFSEDPSCFAAVIPKIHAFADTVPGLRLKI
                     SPSQFYLNIDLEGVTKGSAIRTLAEEFGLRREEMAGIGDTLGDLSIRESVGFFACPSN
                     AVPALKEVADYISPFPDLRGVLDILDHPKMQRH"
     gene            952..2190
                     /locus_tag="PKN92_13215"
     CDS             952..2190
                     /locus_tag="PKN92_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49798.1"
                     /translation="MTHPGMDRRHFLYSAAAGVGLAAALQGAASQAESASAKNAYHRW
                     IHGLGGDDPAATAAEMKACGFERVVANGEAAIEAINGAGMEAWMCGGGFSLIRNEDAL
                     RSRDIFGQPQDWFGSGSPCCDEIREASLESYRKMAQTPGVKGILVDGVRFASPASGLL
                     PFLTDFSGHAERRARELDMDFALMKRDVTKLHDLLTGSGLADAAVRFGSAAPTAWLEE
                     LGRLPGLLEWLRFRRVCTTAHFRALSEIIHGAGLRMGVYIFTPCLAPLVGQSYEDLKS
                     FVGVFAPMIYRNYPDHPGPACLNWELAELPDVLGVSGTAGEKAVMEAVLNLTGFAGLM
                     DAPSVEAVKKALPPQAVALETRRARALIGDEKELAPIIYIDDPLMAETAAGVRAAGAD
                     GVNFFVYKDQWKEMTAPALH"
     gene            complement(2263..>2587)
                     /locus_tag="PKN92_13220"
     CDS             complement(2263..>2587)
                     /locus_tag="PKN92_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49799.1"
                     /translation="SYVGMRTEDILTAAHWLKTKEDKAIRLIATGEATVPARHAAALE
                     PDLFKMLRLEGGIPSWAEVVHTTRAKHQLINTVHNALAWYDLPDLTALTQAQVEEISS
                     TVPEF"
BASE COUNT          508 a          667 c          782 g          630 t
ORIGIN      
        1 ttttatatgg cttgacgtat ctgcttccgg taacattata gagggcttga ttcgggctct
       61 gtgaatgaaa gcgtaccatg atatactttg aggactgtgt gcggtgcggc aggatccttt
      121 tctgccattt tgccgctgcc ttcttcctat ccattcttat tacggagacc tgtatttatg
      181 agcgtccgac tgatagtgtc tgatgtagac gggtgtattt ctcccgaaga gtcgtgtgcc
      241 tgggacggcg aacttttcca cacttttgcg gagcgctgcc gtcgtgcttc ttcaggagaa
      301 agcacgctgg cgccgctcac cctgtgtacc ggcagaccgc agccttatgt ggaagtgttg
      361 atgaaactgc tggacatatg tttcccggct atctgcgagt cgggcgcggt aatctaccgg
      421 ctggacagca atctctccag tttcgcctca tccatcacaa cagaaatgat tcaggggctt
      481 caccgggtgc gggattatat cacggccgag attctgcctg ccgttcccgg gctgctctat
      541 cagttcggca aagaagcgca gatgtcgctt ttttctgagg atccttcctg ctttgcggcg
      601 gtgataccga aaatccacgc ctttgctgat accgtgccgg ggcttcggct caaaatttcg
      661 ccgagccagt tttatctgaa tattgatctt gaaggggtga ccaaaggatc ggcgatccgg
      721 acgctggcgg aagaattcgg cctccgccgg gaagagatgg cgggcatcgg tgatacactg
      781 ggcgatctgt ccatccggga atcagtcgga ttttttgcct gcccgtcaaa tgcggttccg
      841 gcgttgaagg aagtggcgga ttacatttca ccttttccgg atctgcgggg cgtccttgat
      901 attcttgatc acccgaaaat gcagcggcat taaatggaag gaataacagt aatgacacat
      961 ccgggtatgg acagacggca ttttctttac agtgcggcgg cgggtgtcgg tctggcggcg
     1021 gctttacagg gtgcggcgtc tcaggcagaa agtgcatccg caaagaatgc gtatcaccgg
     1081 tggatccatg gtctgggcgg cgacgacccg gcagccacgg cagcagaaat gaaagcatgc
     1141 ggttttgagc gtgtggtcgc caatggcgag gctgctattg aagcgatcaa tggtgccggg
     1201 atggaggcct ggatgtgcgg cggcgggttt tcgctgatcc ggaatgagga cgccttgcgt
     1261 tcccgggata ttttcggaca gcctcaggac tggttcggct ccggatcacc ctgctgcgat
     1321 gaaatcagag aagcctcgct cgagagctac cggaaaatgg cacaaacacc cggggtcaaa
     1381 gggattctgg tcgacggcgt acgctttgct tctccggcaa gcgggctcct tccgtttctg
     1441 accgactttt ccgggcatgc ggagaggcgt gcccgtgaac tggatatgga ttttgcattg
     1501 atgaagcggg atgtgacaaa gctccatgac ctgctgacgg gctccgggct ggcggatgct
     1561 gccgttcgtt tcggatctgc agcgccgacc gcctggctgg aggagctcgg cagactgccg
     1621 ggacttcttg aatggctgcg gttccggcgt gtctgcacga ctgcgcattt tcgtgcgctg
     1681 tcggagatta tccatggcgc cgggcttcgt atgggtgtgt acatttttac accctgcctc
     1741 gcaccgctgg tgggacaatc ctacgaagat ctgaaatctt ttgtgggtgt ttttgcgccc
     1801 atgatctaca gaaactatcc ggatcatccc ggccctgcct gcctgaactg ggaactcgcc
     1861 gagctgcctg atgtgctcgg tgtttccgga acggcgggtg aaaaggcggt gatggaagct
     1921 gttctgaacc tgacaggttt tgccggactt atggatgcgc cctctgtcga agcagtcaaa
     1981 aaagcgctgc ctccgcaagc ggtggcactg gagacccgtc gtgcccgtgc acttatcggc
     2041 gatgaaaaag aacttgcccc cattatttat attgacgatc cgctcatggc ggaaacagcc
     2101 gcgggtgttc gtgcagccgg agcggacggt gtgaactttt tcgtctataa agaccagtgg
     2161 aaagaaatga cagctccggc attgcactga cagcagtatg cagccggaaa cgcctctttt
     2221 cagccggatt gcggtataag gaaaaatcga aagcggcgct gcttagaatt ccggcacagt
     2281 gctgctgatt tcttccacct gcgcctgagt cagcgctgta aggtcgggca ggtcatacca
     2341 ggcaagggca ttgtgaacgg tattgatcag ctgatgcttt gcccgggtcg tatgcaccac
     2401 ctctgcccag gaggggatgc cgccctccag acgaagcatt ttgaacagat ccggttccag
     2461 agctgcggca tgccgtgccg gaacggttgc ctcgccagtc gctatcagcc ggatcgcttt
     2521 gtcttctttg gtcttcagcc aatgcgcggc ggtgagtatg tcttcggtgc gcatgccgac
     2581 atagctg
//
LOCUS       DAOPWB010000237         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_42351_length_2566_cov_3.678216, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000237 DAOPWB010000000
VERSION     DAOPWB010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42351_length_2566_cov_3.678216"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PKN92_13225"
     CDS             complement(<1..342)
                     /locus_tag="PKN92_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HNZ49800.1"
                     /translation="MSKNTSQIPDDQQYALHPYWREKKAKKWLTRLILFSIILHVAVI
                     LLLGLLSKFHFKKPEVEVLMLSGGVTISENAAETPANPEPQPEVKPEPPKPEPPKPEP
                     PKPEPPKPEPPK"
     gene            782..2332
                     /locus_tag="PKN92_13230"
     CDS             782..2332
                     /locus_tag="PKN92_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNZ49801.1"
                     /translation="MKRYCCLFSLTMLFALLFTSAYGAVSTPSIFGDHMVLQQKKTIP
                     VWGKADPGESVTVTLGDVSRKATACGEGRWRVDLPKMKAGGPYELKIKGAGNSLLFSD
                     VLVGEVWIGSGQSNMQWTVADSNNAEREIANAQYPKIRLFDVKRTVATSPQDDCTGEW
                     KVCSPETLPSFSAVLYYFGRELHQQLNDMPMGLIHTSWGGTPAESWTSRPMLESDPQL
                     VGLVKKWDKTLADYPQAKAAYDEALAVWEAEAEKAKAEGKEAPKKPKAPEGPDHPWLA
                     SGLYNAMIAPLVPYAIQGAVWYQGESNANRAYQYRTLFPAMINDWRNAWGQGNFSFYF
                     VQLANFTEVKEKPADSDWAELREAQTMALSLRKTGMATIIDIGEADDIHPRNKQDVGK
                     RLAFNALAKDYGRQIIFSGPMYKNMQIRNGAIVLSFNHAHGGLRAADNGELTGFAIAG
                     SDKKFVWADAVVRGKTVVVSSPDVKEPVAVRYAWANNPVCNLVNGAGLPASPFRTDEW
                     PGLTDNNE"
     gene            complement(2383..>2566)
                     /locus_tag="PKN92_13235"
     CDS             complement(2383..>2566)
                     /locus_tag="PKN92_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyketide synthesis methyltransferase"
                     /protein_id="HNZ49802.1"
                     /translation="DKISPPAGALFAVNMLVSTDGGNCYTFAEIQAALEKAGFTHAAL
                     LQEGSKMDGVVEGFKA"
BASE COUNT          579 a          635 c          724 g          628 t
ORIGIN      
        1 cttcggcggt tccggtttcg gcggttccgg tttcggcggc tccggcttcg gcggctccgg
       61 cttcggcggc tccggcttca cttcgggttg cggttccggg ttggcaggtg tttccgcagc
      121 attttcggag atggtcaccc cgccggacag cataagcact tccacttcag gttttttaaa
      181 gtgaaatttt gacagaagcc ccagcagcag aattactgcc acatgaagaa taatggagaa
      241 gagaatcagc cgggtgagcc attttttggc tttcttttca cgccagtaag ggtgaagtgc
      301 atattgctga tcatctggta tttgtgatgt gtttttactc attgtctctg ctgttgtgcg
      361 tacaggagtt gaggcgccgg aaagttggtg tcccactgtc cggcattcga cagctgcgcc
      421 gaaccatggg aagggagcgg ttccatgccg catcctcccg atgtgccgga caggtggaag
      481 cgtggagcag caatctgtac ggcctgcggg tacgaacaaa ttttgatttt tgctcattcc
      541 ctcttttcag tgcgattagt atactaaaaa aacgatacac aaaggaattc aggttgcgga
      601 ctcgctgaac aaaggcgtac cgaaaaagat ctgttgcatt tatctgcggt ctctccgtac
      661 aattgccttt tgtgccgtgt gtctgtcttc atgcagaccc tatcggcgga agaactgcgg
      721 atacccgatt ttaaacatgc tgcatggctc catgtggcgc aacttttatg gagagttgat
      781 tatgaaacgc tattgctgcc tgttttcgtt gacgatgctg tttgcgctgt tgtttacaag
      841 tgcctatgga gcggtcagta cgcccagcat attcggagat catatggttc tgcagcagaa
      901 gaagacaatt cctgtctggg gaaaagccga tcccggagaa agtgtaaccg ttactctggg
      961 cgacgtttcc cgaaaagcca cggcgtgcgg ggaaggcaga tggcgtgttg atcttcctaa
     1021 aatgaaggcg ggcggaccct atgaattgaa gattaagggg gcaggcaaca gccttctttt
     1081 ctccgatgtg cttgtgggag aagtctggat cggctccggt cagtctaata tgcagtggac
     1141 tgtggcagat tcgaacaatg cggagcgcga aattgccaat gcgcagtatc cgaaaatccg
     1201 tctttttgat gtaaaacgca ccgtggcgac atcgccgcag gatgactgta ctggcgaatg
     1261 gaaagtctgc tcgcctgaaa ccctgccgtc tttttccgct gtcctttatt acttcgggcg
     1321 tgaactgcac cagcagctga atgatatgcc gatgggtctg attcatacgt cctggggcgg
     1381 tactcctgca gaatcctgga ccagcagacc tatgcttgaa agcgatcccc agctggtggg
     1441 attggtcaaa aaatgggata aaacgctggc tgattatcct caggcaaaag ccgcctatga
     1501 tgaagcactg gcggtgtggg aagcggaagc cgagaaagca aaggcagaag ggaaagaggc
     1561 acccaaaaaa cccaaggctc ctgaaggccc cgatcatccc tggctcgctt ccgggcttta
     1621 caatgccatg attgcacccc ttgttcccta tgccattcaa ggagccgtct ggtatcaggg
     1681 cgagtccaat gccaaccggg cataccagta cagaacgttg ttccctgcca tgatcaatga
     1741 ctggcgcaat gcgtgggggc agggaaattt ctccttctat tttgtgcagc tggcaaactt
     1801 taccgaagtg aaagagaagc cggcggactc tgattgggcg gagctgcgtg aggcgcagac
     1861 catggcgctt tccctcagga aaacaggcat ggcgaccatt atcgatattg gcgaagccga
     1921 tgacattcat ccccgcaata aacaggatgt gggcaagcgc ctggccttca atgcactggc
     1981 aaaagactat ggcaggcaga tcatcttttc cggtcctatg tataagaata tgcaaatccg
     2041 aaacggtgcc attgtgctga gcttcaatca cgctcatggc ggactgagag cggcggacaa
     2101 tggtgagctg accggttttg ccattgcggg cagcgataaa aaattcgtct gggcagatgc
     2161 ggttgtccgc ggaaaaacag tagtggtctc ttcgccggac gtgaaagagc ctgttgctgt
     2221 ccgttatgcc tgggcaaaca acccggtttg caatctggtc aacggtgcag ggctgcctgc
     2281 atcgcccttc cgcactgatg agtggcccgg tttgacggac aataacgaat agccggctgc
     2341 tccgctcact ctatttacgg cgtggttgac gggttctctg cctcaggctt tgaacccttc
     2401 gactacgccg tccattttac tgccttcctg caggagtgcg gcatgcgtga atccggcttt
     2461 ttccagagct gcctgaattt ccgcaaaggt atagcagttg ccgccgtccg tggaaactag
     2521 catattcaca gcgaaaagtg caccggccgg aggactgatt ttgtcc
//
LOCUS       DAOPWB010000238         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_42843_length_2545_cov_3.686747, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000238 DAOPWB010000000
VERSION     DAOPWB010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42843_length_2545_cov_3.686747"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PKN92_13240"
     CDS             complement(<1..1244)
                     /locus_tag="PKN92_13240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNZ49803.1"
                     /translation="MKGQTYAAALESLYGQVEKPGRYIGSEWNAAVKDPARIKLRLCL
                     VFPDLYELSLGNLGLQILYRIFNAMPEVWAERAYLPGPDMDALLRERHLAPPMLESQE
                     PLTAADAIGFTLQSELTYVNILRMLDLAGLPLRTEKRGEEAPLVFAGGPCAYNPEPLA
                     PFIDFFVIGDGEEAAQEIAACLLSMRGRSKAAQLEALAALEGVYVPSLFPVEALCDGS
                     VVPVASGRKIRSRLVQDLNGIPFPDAPITPYVQLVHDGYGVEVLRGCSHGCRFCQAGM
                     VTRPVRERSVDTISGLLDKGLARSGFEDATLVSLSTCDYSVAPSLLHTSADVAEKHRA
                     SLSLPSLRLDRFSVQLADHVASMRRSGLTFAPEAGSLRLRRVINKDVTDEQLLELAEE
                     VFRRGWMHIKLYFMIGLPTEQE"
     gene            complement(1274..2257)
                     /locus_tag="PKN92_13245"
     CDS             complement(1274..2257)
                     /locus_tag="PKN92_13245"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNZ49804.1"
                     /translation="MPYSLDYIYGAISPTFTVFHEDGRLDDDGQRRFVDFLLQDNAIS
                     AYFVRSGMGLMYTFSMEDTLQIARNMCAHLKGIRPVLVGCSGIWDRNYDKRPDPDLYL
                     QQGIKLGNETLEMGAAAAVYTIPEALVPKEGQRHQDLIETYFTTLCAQVNGPVMVYQP
                     PGTRKEYEIAPEVLRRLTALPNFVAGKFSTSEGYYAYELQRAVRGCDFRYIVGNETML
                     YIGVMLGSRACIGQGAIINPQMLNALVDRYLTGNHAGVLAAQDAINNLLKPNIHAVEF
                     LKRYATEKGFDTPLYSRSQKTNPYASDRAAMTEGEYQNFKKLYELEMQAFR"
BASE COUNT          573 a          753 c          683 g          536 t
ORIGIN      
        1 tcctcctgtt ccgtgggcag cccgatcatg aagtacagtt tgatgtgcat ccagccccgg
       61 cggaaaacct cttccgcaag ttcgagcagc tgttcgtccg tgacgtcttt gttgatgacg
      121 cgccggagcc gcagcgatcc ggcttcgggg gcaaaggtca gcccgctgcg ccgcatcgat
      181 gccacgtgat ccgccagctg cactgaaaaa cggtcgagcc gaagcgaagg aagcgacagc
      241 gatgcacgat gcttttccgc aacgtccgcc gaagtgtgca ggagggaggg cgcaacagaa
      301 taatcgcagg tcgaaagaga caccagtgtt gcatcttcaa agcccgaccg ggcaagccct
      361 ttatccagca gcccgctgat cgtgtccacg gaacgctccc ggacagggcg ggtcaccatg
      421 cctgcctggc agaaacggca gccgtgactg cagccacgca ggacttccac gccgtagccg
      481 tcgtgtacca gctgtacata aggcgtaatc ggcgcatcag gaaaggggat gccgttcaga
      541 tcctgcacca gccgtgaccg aatttttctt ccggacgcaa ccggcactac gctgccgtca
      601 cacagggcct ccacaggaaa aagggaaggc acatagaccc cttccagtgc cgccagcgct
      661 tccagctgtg ccgcttttga tcttccccgc attgacagca ggcaggcagc gatttcctgc
      721 gctgcttctt cgccgtcgcc gatgacaaaa aagtcgatga agggcgcaag cggctcagga
      781 ttgtaggcac agggaccgcc ggcaaaaacc agcggtgctt cttcgccccg tttttctgta
      841 cgcagaggca gtcccgcgag atccagcatg cgaagtatat tcacataggt cagttcagac
      901 tggagcgtaa atccgatggc atcggcagca gtcagcggtt cctgcgattc cagcatgggc
      961 ggcgccagat gccgttcccg cagcagcgca tccatatccg gaccggggag ataggcacgc
     1021 tccgcccaca cctccggcat ggcgttgaaa atccggtaaa gaatctgcag ccccagattg
     1081 cccagcgaca gctcgtacag gtcgggaaac accaggcaca ggcgaagttt tatccgggcg
     1141 gggtctttga cagcagcatt ccattcgctg ccgatataac gacccggctt ctccacctgc
     1201 ccgtagagcg attccagggc agcggcataa gtctgacctt tcaaaaggga aactcccgat
     1261 gacggctccc ggcttaacgg aaagcctgca tctccagttc atacagtttc ttgaagttct
     1321 ggtattcccc ttccgtcatc gcagcccgat ccgatgcata tggattcgtt ttctgtgacc
     1381 gggaatagag cggtgtgtca aagccctttt ccgtcgcata ccgcttcaga aattccaccg
     1441 catggatatt cggcttcaac aggttgttga tcgcatcctg tgccgcaagc acgcccgcat
     1501 gattgcccgt cagataccgg tccaccagcg cattgagcat ctgcggattg ataatggcgc
     1561 cctgaccgat gcaggcgcgg ctgcccagca ttaccccgat atacagcatg gtttcattgc
     1621 ccacgatata acggaagtcg cagccccgta ctgcccgctg cagctcataa gcgtaatagc
     1681 cttcggaggt cgaaaatttg cctgccacaa aattcggcag tgctgtcagg cgccggagta
     1741 cctcgggtgc aatctcgtat tcttttcggg tgcccggcgg ctgatacacc atcaccggcc
     1801 cgttcacctg agcgcacaga gtcgtgaaat aagtctcgat cagatcctga tgcctctgtc
     1861 cttctttggg aaccagcgct tcaggaatgg tatacacggc ggccgccgca cccatctcca
     1921 gcgtttcatt tcccagtttg atcccctgct gcagataaag atccggatcc gggcgtttgt
     1981 cgtaattccg gtcccagatg ccgctgcagc ccaccagtac tgggcggatg cctttcagat
     2041 gggcgcacat gttccgggct atttgcagcg tgtcttccat gctgaaggtg tacataaggc
     2101 ccatgccgga acgcacaaaa tatgcgctga tcgcattgtc ctgaagaaga aaatcgacga
     2161 accggcgctg tccgtcatcg tccagccttc cgtcctcatg aaatacggtg aaagtggggg
     2221 aaatggcacc atagatataa tcaagtgaat atggcatgga aatctgtcct atgaaccgcc
     2281 gatgcggccc ggcttgaaag aaaaggaaag tgcgccccta tcaggcatcc ggaatatgat
     2341 gccacaacca tgccgctgca ggcaattggt tctggaggat attcgaggtc tatttttttg
     2401 gagaatgccc cgggacatga tacagcgttt agcggagctt ctttcttgtt taattgcctg
     2461 agatttattt tgcccctgca ctaaaacact ctgactcaaa acataaaatc cgccagcctg
     2521 ggaacgccaa tctccgtatt ggcaa
//
LOCUS       DAOPWB010000239         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_43409_length_2521_cov_4.207624, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000239 DAOPWB010000000
VERSION     DAOPWB010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43409_length_2521_cov_4.207624"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PKN92_13250"
     CDS             <1..1056
                     /locus_tag="PKN92_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNZ49805.1"
                     /translation="VLDQDGGAAMYKSLLKQWSRRSTAIVLDFASTDSIDSLGGAWIM
                     KAAGYARQKGVDFSWKGQHDALSDFLEMLAPSLQASPPARPKMPGPLEKLGEVIISVT
                     KECGEFLSLTVDTIYWTLIAPFEGRGFRFNAFIDEVHEMGVRAVRIVCLMNFLLGLII
                     AMLSAKQVEAFGIQIYVANLIFIGFARELAAVMTAVVVSARSGAAIAAELATMTVQEE
                     IDALRGMGISPAQYLVTPKLLAMLVALPCLATLGLISGVLGGAVWGIFVLGFRPFMWY
                     NQTLEAAAMDDIMQGMLKTFFFAIAIVMIGCHNGFRVKGGSRGVGLMTTRAVVMDIFS
                     LICIDIVFASIFYYLLG"
     gene            1074..1844
                     /locus_tag="PKN92_13255"
     CDS             1074..1844
                     /locus_tag="PKN92_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNZ49806.1"
                     /translation="MSETMISVENLVSHYGPVKVLDGVTFDVKKGEIFMVIGGSGCGK
                     STLLKQMCGLLKPTSGHIIYRGKDLAAMDEDELSAMQRTIGIAFQSSGLFNSMTVGEN
                     VAMPMREFGGVDPKLIDELVRYKLSLVGLAHAQHLMPSELSGGMRKRAGLARAIALDP
                     DLVYFDEPSAGLDPIMAAGLDNMILKLKSLLNTTFVIVTHELDSIRLAADRILMLDMG
                     EVIFCGTLEEAAASPVDRLRQFFDRRADDFIVQRNITS"
     gene            1874..>2521
                     /locus_tag="PKN92_13260"
     CDS             1874..>2521
                     /locus_tag="PKN92_13260"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNZ49807.1"
                     /translation="MATRKQKIKVSLFLIICVAIMAVSAAVISGFYKEPGISYWMEFN
                     ESILGLYEGGMVEYLGVPVGKITDIYVTSDQVAHVEIIINPQKVTLHEGVQGQLVIYS
                     IASGTMAISLSGGNKEGERLHEYAQIPTKTSTIEAFSTQLSEILSDVSEISGSIKGQL
                     AHLDETAVKNVVDQVQTLLGKGETFVEDTTMLVKESTETVQDIRSHASTLVSSLET"
BASE COUNT          577 a          662 c          690 g          592 t
ORIGIN      
        1 gttctggatc aggatggcgg tgccgccatg tacaagtcac tgctcaaaca gtggagccgg
       61 cggagcaccg ccattgtcct cgactttgct tccaccgaca gcatcgattc tttgggcggc
      121 gcctggatca tgaaagcagc ggggtacgcc cggcagaaag gcgttgattt ttcctggaaa
      181 ggacagcacg acgcccttag cgattttctg gaaatgctgg cgccctccct gcaggcatcg
      241 cctcctgcac gcccgaaaat gcccgggcca ctggaaaagc tgggtgaagt cattatctcc
      301 gtcacgaaag aatgcggcga gtttctctct cttactgtgg acacaatcta ctggacgctg
      361 attgcaccct ttgaaggcag aggtttccgc ttcaacgctt ttattgatga ggttcatgaa
      421 atgggggtgc gtgccgtgcg catcgtatgt ctcatgaact ttctgctcgg tctgattatt
      481 gccatgcttt ccgccaaaca ggtggaagca ttcggcattc agatctatgt tgccaacctg
      541 attttcatcg gtttcgcccg ggaactggct gccgtcatga cggctgtggt tgtatcggca
      601 cgcagcggcg ccgccattgc agcggaactg gcaacgatga ccgtgcagga ggaaatagat
      661 gcactgcgcg gcatgggcat cagcccggcg cagtatctgg tcacgccgaa actgcttgcc
      721 atgctggtgg cgctgccctg ccttgccacg ttgggcctga tctcgggtgt gctgggcggt
      781 gccgtctggg gcatttttgt tctcgggttc cgccccttta tgtggtacaa ccagacactg
      841 gaagcagcag ccatggacga tatcatgcag ggcatgctga aaaccttctt cttcgccatc
      901 gccattgtaa tgatcggctg ccacaacggc ttccgggtaa aaggcggatc cagaggcgta
      961 gggcttatga ccacccgtgc tgtggtcatg gacatctttt ctctgatctg cattgatatt
     1021 gtatttgcaa gtattttcta ctatctgctg ggctaaacat gccggacttt tctatgagtg
     1081 aaaccatgat cagcgttgaa aatctagtct cccactacgg accggtcaaa gtgctggacg
     1141 gtgtgacttt tgacgtcaaa aaaggagaga tattcatggt catcggcggc agcggatgcg
     1201 gcaaaagcac gctgctcaaa caaatgtgcg gcctgctgaa acccaccagc ggacatatca
     1261 tataccgggg caaagatctt gccgccatgg atgaagatga gttgagcgcc atgcagcgca
     1321 ccatcggcat tgcatttcag tccagcggtc ttttcaactc catgactgtg ggtgaaaacg
     1381 tcgccatgcc catgcgggaa ttcggcggtg tggatccgaa gttaattgat gagctggttc
     1441 gctacaaact cagtctggtg gggcttgccc atgcgcagca ccttatgcct tccgaactgt
     1501 cgggaggcat gcggaaaagg gcgggtctcg cccgcgccat tgcgctggat ccggatctgg
     1561 tttatttcga tgagccttcc gccggtcttg accccatcat ggcggccggg cttgacaaca
     1621 tgattctcaa attgaaatcg ctgctgaata caacctttgt catcgtgacc catgaactgg
     1681 attccatccg tctggcggca gatcgtatcc tgatgctgga catgggcgaa gtaattttct
     1741 gcgggacact ggaagaggcg gcagcctcac cagtcgaccg gctgaggcag ttttttgacc
     1801 gtcgtgccga tgacttcatt gtccaacgca atatcaccag ctgaatacag gcaaagaaaa
     1861 ggaaaacaga caggtggcta ctcgcaaaca gaaaatcaaa gtcagcctct tcctgattat
     1921 ctgcgtcgcc atcatggcgg tgtccgctgc ggtcatttcg ggattctaca aagaacccgg
     1981 catctcgtac tggatggaat tcaatgaatc cattctgggg ctctacgagg gcggcatggt
     2041 cgaatatctg ggcgtgccgg tcggtaaaat caccgacatc tatgtaacct ccgatcaagt
     2101 cgcccatgtg gaaatcatca tcaatccgca gaaagtaacc ctccatgagg gcgtacaggg
     2161 ccagctggtc atctacagca tcgcttccgg caccatggcg atttcactga gcggcggaaa
     2221 caaggagggt gaacgccttc atgaatatgc acaaattccg accaagacca gcacgataga
     2281 agccttcagc acccagctgt ctgaaattct ttcagatgtc tctgaaattt ccggatccat
     2341 caaaggacaa ctggcgcatc tggacgaaac tgctgtgaag aatgtcgttg atcaggtaca
     2401 gacgcttctg ggcaaagggg aaacttttgt tgaagacacc accatgcttg tgaaggaaag
     2461 cacggaaacg gtgcaggata tccgcagcca tgcaagcact cttgtttcca gtctggagac
     2521 c
//
LOCUS       DAOPWB010000240         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_44769_length_2468_cov_4.428098, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000240 DAOPWB010000000
VERSION     DAOPWB010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44769_length_2468_cov_4.428098"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PKN92_13265"
     CDS             <1..511
                     /locus_tag="PKN92_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HNZ49808.1"
                     /translation="PEPKPEPKPEPKPEPKPEPKPEPKPEPKPEPKPEPKPEPKPEPK
                     PEPKPEPKPATPSNPQELKLTTGEISEGIPSVLSGWVRLVQRKVEGVWAVPPGIMMDP
                     DNNIAEVSFWVDRYGDLIGTPVVTKHAADPALGESGINAIMLAQPFPPLPEGYPQVEQ
                     LVVYAFKLH"
     gene            562..1989
                     /locus_tag="PKN92_13270"
     CDS             562..1989
                     /locus_tag="PKN92_13270"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNZ49809.1"
                     /translation="MIRLFPAVLALCFFAGTAVADSTVTRHFDDNGAALVNPMMGWRL
                     NFYSNMLSNYGSTLAFSDTLDDFPGLSCIYMRLPWVYIEAEEGVFDWSVVDAPAQRWI
                     DKGLQVAFRFCCSESWLRYATPQWVKDAGAKGYDFVPGQGIVEDGVFWEPDYNDPIFL
                     DKLEKFVKAAAARYDGNPHVAFVDVGSFGVWGEGHTDASTRILYPADVVKKHIDLYAR
                     HFKKTLLAANDDFSFQGDTILPYAFEKGLTLCDDSILVQPPPNSYYSAALAQPFWPVR
                     PVILEHQHYDTSKSWGAWHPDIFLRAIEEYHAAYMCIHWFPREFLEQERELIDRVNLR
                     LGYRLQVREVQWQPEAHLHERISFRSSWANAGVAPCYPHGWVAYTLKDAQGGIAAVFA
                     DEHFDMFDLPVGAAGSAVIKETQSSHSFAPNMPPGKYDLFISAGLRDGTPVIALPLEK
                     EDGHRRYFVGTMTVLEAKTGAPAEE"
     gene            2225..>2468
                     /locus_tag="PKN92_13275"
     CDS             2225..>2468
                     /locus_tag="PKN92_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HNZ49810.1"
                     /translation="MAMTWIDYASILAFIALVLGVSMYKSRQKKDSAGYFLAGREATW
                     WLIGFSLIAANISTEQFVGMSGAGAGNTGLAIASYEW"
BASE COUNT          544 a          691 c          645 g          588 t
ORIGIN      
        1 gcccgagcct aagcccgagc ccaagccaga accgaaacct gaaccgaagc cggaacccaa
       61 acctgaaccc aaacctgaac ccaaacccga gcccaagccg gagcccaagc cggaacccaa
      121 gccggaaccc aagcctgagc caaaacctga gcccaagcct gcgacgcctt ccaatccaca
      181 ggaattgaaa ctcaccacag gcgaaatttc tgaaggcata ccttctgttc tcagtggatg
      241 ggtgcggctg gttcagcgca aagtggaagg tgtctgggct gttccgccgg gtattatgat
      301 ggacccggac aacaatattg ccgaggtcag tttctgggtg gatcgctacg gcgatctgat
      361 cggcactcct gtcgtgacaa aacatgcggc agatcctgca ctgggcgagt ccggcatcaa
      421 tgccattatg cttgcccagc ccttcccgcc gttgccggaa ggatatcctc aagtggagca
      481 gctggtcgtt tacgccttta aactgcactg atctcccgca caactgcctt gttttccgtt
      541 gcatcctgaa aggagtattg catgatcagg ctttttccgg ctgtcctcgc tctttgtttt
      601 tttgccggaa cggctgtggc tgacagcacg gtcaccaggc attttgacga caacggtgcg
      661 gcgctggtaa atccgatgat gggctggcgt ctcaattttt attccaacat gctgtccaac
      721 tacggttcca cactcgcctt ttcggacacg cttgacgatt ttccggggct gtcctgcatt
      781 tatatgcggc tgccctgggt ctatatcgaa gcggaggaag gcgtgtttga ctggtctgtc
      841 gtggacgcgc cggcacaacg ctggatcgat aaaggactgc aggtcgcttt tcgcttctgc
      901 tgctccgagt cctggctccg ctatgccacg ccgcaatggg taaaagacgc cggtgcaaag
      961 ggatatgatt ttgtgccggg gcagggcatc gtggaagacg gcgttttctg ggaaccggac
     1021 tacaacgacc ccatctttct tgacaaactg gaaaaatttg ttaaggctgc cgccgcccgt
     1081 tatgacggca acccccatgt agccttcgtc gatgtcggct cgttcggtgt atggggcgaa
     1141 ggacacaccg atgccagcac acgcattctc tatccggcag atgttgtgaa aaagcatatc
     1201 gatctttacg cacgccattt caaaaaaaca ctgctcgcag ccaacgacga tttcagtttt
     1261 cagggtgata caattctccc ttacgctttt gaaaaagggc tgactctctg cgatgacagc
     1321 attcttgtgc agccgcctcc caattcctat tacagcgctg cactggcgca gcctttctgg
     1381 ccggtacgcc ctgtcattct ggagcatcag cattatgaca cctcaaagag ctggggtgcc
     1441 tggcatcccg atattttctt gcgcgccatt gaagaatatc atgcggctta tatgtgcatt
     1501 cactggtttc cccgtgaatt ccttgaacag gaaagagaac tcattgaccg cgtcaatctg
     1561 cggctgggct atcgtctgca ggtccgggaa gtgcaatggc agccggaggc tcacctgcat
     1621 gagcgcatct ctttccgttc ctcctgggca aacgccggcg tagccccctg ctatcctcat
     1681 ggatgggtcg cctacacgct gaaagacgct cagggcggta ttgccgccgt ttttgcggat
     1741 gagcactttg acatgttcga cctgcctgta ggcgccgccg ggagcgctgt tataaaagaa
     1801 acacagtcca gccacagttt cgcccccaat atgccgcccg ggaaatacga ccttttcatt
     1861 tcggcgggtc tccgtgacgg cacgcccgtc attgcccttc ctctggaaaa agaagacggg
     1921 caccggcgct acttcgtcgg taccatgacc gttctcgaag caaaaacagg cgcccctgca
     1981 gaggaataac tttttattct gcccagaggg ctgcttcgag atctgctgaa aagagagaac
     2041 cggctcccct ttatcgcatt cactccggct tccgccctta tctcgccact agaaaggggc
     2101 gttccttttc cgttacaatc ctgtcggaag tgattcagga tctctgtatg tatttttctg
     2161 ctcggcagga caattgtggc ataaaacatt ttaattttgg agcactaaac aggagcgcat
     2221 tattatggca atgacatgga tagactatgc cagtattctg gcgtttattg cacttgttct
     2281 gggagtgagc atgtacaaaa gccgccagaa aaaggacagt gcaggctatt ttctggcagg
     2341 gcgggaagcg acctggtggc tcatcggctt ttcgctgatt gccgccaata tttcgaccga
     2401 acaatttgtc ggcatgagcg gtgccggtgc cggtaacaca ggacttgcca ttgccagcta
     2461 tgaatgga
//
LOCUS       DAOPWB010000241         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_45609_length_2437_cov_4.646935, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000241 DAOPWB010000000
VERSION     DAOPWB010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45609_length_2437_cov_4.646935"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1919)
                     /locus_tag="PKN92_13280"
     CDS             complement(66..1919)
                     /locus_tag="PKN92_13280"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNZ49811.1"
                     /translation="MRHGTNLLASMEGMSRTAFLIVQELSQAGRSGLTPALLAKKLEI
                     PQEEVEYFVDVHHGVMYTNLTRICLSSGGLGLARRVQGGLASRGDASFLLEHVRALGD
                     AALQRLEERLGLDGSASKTALALAVLHLLYKTPATIETHIRTGAYSPLARSVFQRLLE
                     SDTGSIRMETLRREFAAGSTALEAALQELISDYVLFEKFRFDDEGFLYRSFSLLQELR
                     DFLFSGNDERPAAADMPPPHKGVIEEQRSEGMCFTDLLGRITAAIAARPCRIRKGSTS
                     LDSDAALFKEDRARLEEIISEDHDPPLRAFMDAAYQLGWIKNTDNLCVVCDVAPLLEF
                     SLLERHRLVVRRLTGGQHASDYGLLIRASATLQPEQWYSIHDFARFALSQDDSTGTYA
                     LRRTDTGYAYVAPQPARMRYETLCRLLSQHLTWLGLVERCYCDGEACFRVSALGRRLF
                     SDPPSAGHAEGYSVSSGTLVVQPNFEIMAALEQMDPLRTIPLHIFAETDGKGSVGVFR
                     LTRERFMRALQQGHDPAAFLAFLKENSRSPLPENVVITLEDWLRSAKRIRLRTYHVIE
                     SDDPMVIAELAHHRKWNGHFTAVNEKKLLRYEGLTRQALEEELEKDGYIIR"
BASE COUNT          583 a          699 c          644 g          511 t
ORIGIN      
        1 cctgcccgaa tcgtgtcatg agatacctct gccgcagacc cttaggagag tgttctgcct
       61 gcctgtcatc gaatgatata cccgtccttt tccaattctt cttccagtgc ctgacgggtc
      121 agcccttcat accggagaag cttcttttca ttgactgccg taaaatgacc gttccatttc
      181 cggtgatgcg ccagctccgc aatgaccatc gggtcgtccg attctatcac atgataggtt
      241 ctcagcctga ttcgcttcgc cgacctgagc cagtcctcca gcgtgattac aacattctcc
      301 ggaagagggc tgcggctgtt ctctttgaga aaagcaagaa aagccgccgg atcatgcccc
      361 tgctgcaatg cccgcataaa ccgctcccgt gtcagtcgaa acacccctac agatcctttt
      421 ccgtctgtct ccgcaaaaat atgcagcggg attgtccgaa gcggatccat ctgctccagc
      481 gccgccataa tttcaaaatt aggctggaca acaagcgtgc ccgaagagac gctgtagcct
      541 tccgcatgac cggcggacgg gggatccgaa aaaagacggc gccccaatgc gctgacacgg
      601 aaacaggctt cgccgtcaca atagcaccgc tcgaccaggc ccagccaggt cagatgctgc
      661 gacaggaggc ggcacagcgt ttcataacgc attcgggcgg gctgcggcgc cacataggcg
      721 tagcccgtat cagtccgtcg cagtgcatac gtcccggtgc tgtcatcctg cgacagggca
      781 aagcgggcaa agtcatggat gctgtaccac tgctccggct gcagggtggc agatgcacgg
      841 atcagcaacc catagtcgga agcatgctgc ccaccggtca gcctgcgaac cacaagccga
      901 tgtctttcca ggagactgaa ttccagcagc ggcgccacgt cacacacgac gcagaggtta
      961 tctgtgtttt tgatccaccc aagctgatag gcagcgtcca taaaagctct cagcggcggg
     1021 tcgtggtctt cggaaatgat ctcttccagc cgcgcccggt cttccttgaa aagcgccgca
     1081 tcggaatcga gagaagtgct gcccttgcgg atgcggcagg gccgcgctgc tatggcagcg
     1141 gtaatacggc cgagcagatc cgtgaagcac atgccttcac tgcgctgttc ttcaatcacg
     1201 cctttgtgcg gaggcggcat gtctgccgca gcgggacgtt cgtcattgcc tgaaaataaa
     1261 aaatcccgaa gttcctgaag caggctgaag cttcgataga gaaagccttc gtcatcaaaa
     1321 cggaactttt cgaaaagaac atagtcgctg atcaactcct gaagcgcggc ttccagcgca
     1381 gtgctgcccg ccgcaaactc acggcgcagc gtttccatgc gaatactgcc ggtatctgat
     1441 tcaagcagcc tctgaaaaac cgatctggca agaggagaat aggcgcctgt ccgaatgtgg
     1501 gtttcaatgg tggcaggcgt tttataaaga agatgcagca cggcaagggc tagcgcagtt
     1561 ttggatgcgc tgccgtcaag acccaggcgc tcttccagcc gctgcagggc agcatcgccc
     1621 agcgcccgga catgctcaag aagaaaagag gcgtcgccac ggctggctag cccgccctga
     1681 acacgtctgg caagtccaag cccccccgaa ctgagacaga tgcgggtcag attggtatac
     1741 atcacaccgt gatgcacatc cacgaaatac tccacttcct cctgaggtat ttcgagtttt
     1801 ttcgccagca gcgcaggcgt cagcccggaa cgccccgcct gagacagttc ctgcacgatt
     1861 aaaaatgcgg tgcgactcat cccttccatt gaggcaagaa ggttcgtgcc atggcgcata
     1921 cagtattctc ccgttaaaag aaagcaaact aaacagccca ctattatagg aaatatacgg
     1981 ggaatgcggc aaatgcatcc ggcaggtatt cctgcgcttt ttatgttcca cgggcggttt
     2041 tgtaatgtgg agatgcccgc accgccgaca aaagccatag gatgcttgta gtgaaatttg
     2101 cagattaaag tccctgtttt tctccgccat gccacaccaa aatcagtgga cagagtggac
     2161 acagtggacg gcggcgcctg gcgtataaag ccgtcatcgc ctctcctggg aacgccactc
     2221 tccgcattgg caagggaaga acaacgagta aacacgaatt tataccggag acttatatgg
     2281 gtttcacacc aaaatgcttt gacttaaaac ataaaatccg ccagcctggg aacgccaatc
     2341 gacctattgg caagaggcag gtgaggacta tgtttttggg aggtatgtcg cagggcatat
     2401 ttccgcagtg gatttgagtg actgccttcg tcatgac
//
LOCUS       DAOPWB010000242         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_47141_length_2381_cov_4.378762, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000242 DAOPWB010000000
VERSION     DAOPWB010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47141_length_2381_cov_4.378762"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PKN92_13285"
     CDS             complement(<1..1371)
                     /locus_tag="PKN92_13285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNZ49812.1"
                     /translation="MHLLISALRPSGFRLEHFALILLLLFHLVMNLWWLRADNHGIQS
                     DEELHMLSARSYYDALFPVNENVSLAERLRNAWEVEVESESPVHPPLLPLTGALVMAL
                     FGCSTDVLAFCSTCFFLLMLPGVYLIARKFLSRRHALYSTAVVSFTPLIYTASRYFMT
                     DYLSMVLCVWAVAVLLRSRVLTSTRCAFFFGLLNGFALLARITSPLYYFLPAFFIFVT
                     GLLESRRNRKMLARLLMNAALILAVTIAIAAPWYISHGEHFYRYWTASELIRQTGSPI
                     AWLSKNKDRTSSVSAPRPRQETPPEKTADDTASTRTAMASHPETPAPAAAEKDAGKIP
                     WRRYPVMVINNGVFLPMFLAGVAGMLCAVAFRRLRSNPGIRLLLLWLLGSWFFLTVVL
                     TFATPRYALQALPALALFSALPFLMLPERRAAALLQGLYLAVLLFQYGNLTVAPYGTL
                     CARALPL"
     gene            1566..>2381
                     /locus_tag="PKN92_13290"
     CDS             1566..>2381
                     /locus_tag="PKN92_13290"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNZ49813.1"
                     /translation="MQTPFYSIIIPTYNSVGTLVPLLDSLEKQKRADTEIIVVDDGST
                     DKTAEAVQPFSVRYFRQEARGGPAAARNRGAREAQGEWFIFTDADTLYEADTLRQIIT
                     AVEKSDGDAFVGTYAGRPANSGFMPRYKALWELITIDEVLLSRGGPFIPYNAWAPRPG
                     IVRREAFENVNGFSESFRGADLEDVEFGYRLIKAGFKIFFCPNIRISHHYPATFCKEI
                     RPFARRCRLWMTLDHPGEFDAAGEGSFRQALTHLFGFSAFLFLVPALLIPYFFI"
BASE COUNT          614 a          640 c          654 g          473 t
ORIGIN      
        1 aagcggcaga gcacgggcgc ataacgtgcc gtagggtgcc actgtgaggt tgccgtactg
       61 aaagagaagc actgccagat aaagcccctg cagcaacgcc gccgcccttc tttcaggcag
      121 catcagaaaa ggaagggcgg agaacagtgc cagtgcgggc agcgcctgaa gggcatagcg
      181 gggcgtggca aaagtcagca ccacggtcag aaaaaaccag ctgcccagca gccacaacaa
      241 cagcaggcgt atccccggat tgctccggag gcgacggaaa gcaacggcgc aaagcatgcc
      301 tgccacgcca gccagaaaca tgggcagaaa cacaccgtta ttgatcacca tcacgggata
      361 acgccgccag gggattttcc ctgcatcttt ttccgcagca gcaggcgccg gagtttccgg
      421 atggcttgcc attgccgtcc gcgtggatgc tgtatcatct gccgtctttt ccggcggtgt
      481 ctcctgccgg ggtcggggcg ctgaaacaga agatgtacgg tctttattct ttgacagcca
      541 ggcgatgggg cttcccgtct ggcggatcag ctccgacgct gtccagtacc ggtaaaagtg
      601 ctcgccgtga ctgatgtacc agggtgcggc aatggcaatt gtaacggcaa ggatcaacgc
      661 tgcattcatc agaagccgtg caagcatctt tctgttccgc ctgctttcca gaagcccggt
      721 cacaaaaatg aagaaggcag gcaaaaagta atacagggga ctggttatcc gggcaagaag
      781 cgcaaagccg ttgagcaatc cgaaaaagaa ggcgcagcgc gtactggtca gaacccggct
      841 gcgcagcaga accgccaccg cccagacgca aagcaccatg gacaggtagt ctgtcatgaa
      901 atagcgggat gccgtgtaga taagcggggt aaaactgact actgccgtgc tgtaaagagc
      961 gtggcgccgg gaaagaaact ttcgggcaat cagataaacc cccggaagca taaggagaaa
     1021 gaagcaagtg ctgcagaaag caagcacgtc ggtgctgcat ccgaaaagcg ccatcaccag
     1081 cgcccctgta agcggcagca acggcggatg aacaggactc tcggactcaa cctccacttc
     1141 ccaggcattc cggaggcgct cagccaacga gacattttcg tttaccggga acagtgcatc
     1201 ataataactg cgggcactga gcatatgcag ttcttcatca ctctggatgc cgtggttgtc
     1261 cgcacgaagc caccaaagat tcatcaccag gtgaaaaaga agcagcagaa tcagagcaaa
     1321 gtgctcaaga cgaaagcccg aagggcgcag tgccgaaatc aataaatgca tgagatatcc
     1381 tttttcgctt ccctgatccg ggaataaagt actatagcaa aaaggtacga aaggaaacag
     1441 agaatcttca gccaccgggt tgcatgtgcg ccgacgaaaa ccgggcactg cttcatggta
     1501 aaatacctcc atgactgacc ggtcagcagg ccgccctgtt tgataaagga aaaaacaaga
     1561 atataatgca gacccctttt tattcgatta tcatccccac atacaattca gtgggtactt
     1621 tagtgcctct gcttgactct ttggaaaagc agaaaagggc agacacggag attatcgtgg
     1681 tcgacgacgg ttccacggat aaaacagcgg aagccgtcca gcccttcagt gtccgttatt
     1741 tcagacagga agcccggggc ggccctgcag cggcacggaa ccggggtgcc cgcgaggcgc
     1801 agggcgaatg gtttatcttt accgatgcgg acacacttta cgaagcggac acgctcaggc
     1861 agatcataac cgctgtagaa aagagcgacg gcgacgcttt tgtcggcacc tatgccggac
     1921 gtccggcaaa ctccgggttt atgccccggt ataaagcctt atgggaatta atcaccattg
     1981 atgaagtact gctttcccgt ggcggcccct tcatccctta taacgcctgg gcaccgagac
     2041 cggggatcgt ccggcgggag gcttttgaaa acgtgaacgg tttcagtgaa tctttcagag
     2101 gggctgatct ggaagacgtt gaattcggtt accgcctgat aaaagcgggt ttcaaaattt
     2161 tcttctgccc gaacataaga atctcccatc attatcccgc caccttctgt aaagaaatcc
     2221 ggcccttcgc ccgacggtgc cgtttatgga tgacgctgga tcaccccgga gaatttgatg
     2281 ccgccggaga agggtcattc cggcaggcgc tgacccattt attcggtttt tccgctttcc
     2341 tttttctggt tccggcgctg cttattcctt attttttcat t
//
LOCUS       DAOPWB010000243         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_47244_length_2378_cov_5.763237, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000243 DAOPWB010000000
VERSION     DAOPWB010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47244_length_2378_cov_5.763237"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKN92_13295"
     CDS             complement(<1..910)
                     /locus_tag="PKN92_13295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNZ49814.1"
                     /translation="MASPEIKHDLMIQFGNEYGPDDEAAVLEVLRKNAPTSGSACIAF
                     EKEFAAYCGVSHARCVSNGTAALFLAMIGIGIKPGDRVITTPLTWIATAAAPVTLGAQ
                     IDFVDVEEGTWNIDPAQIEAAITPETKAIIPVHLYGQCCDMDAINAIAEKHGLAVVED
                     ACHTVGGAYKGRKAGALGKVGCFSFHEQKNISTLGEGGIITTDDADIYERIALYRSHC
                     TRVYGASTKYCSLDESKLPKGKRFWWQDFDDTGYNFRLTDIQAAVGSVQLRKLDMLNQ
                     RRIDNAAYLTEGLKDIPGFRLPEVVEG"
     gene            complement(897..1811)
                     /locus_tag="PKN92_13300"
     CDS             complement(897..1811)
                     /locus_tag="PKN92_13300"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNZ49815.1"
                     /translation="MKKRALLTGFGGFVAGSIAWHCPPEWDLHGTSRKADLPAPAGIQ
                     VHPLELLDTADLERLFKRIRPDVVFHTAAMADIDRCQQNQEAAKAVNAGVTAQLSTLC
                     RDQGARLIFTSTDSIFDGIQGMYRESDAPSPVNYYAETKVQAEETVLSTVLDSVVARL
                     SLVLGFSVLGEGNSFLAKMIHTLESGGEASFPANEIRTPVDVITLGRAFWELAEHSFT
                     GILHLSGCSRLNRYEMAQRIAARLDLPQGKIIAVDSNAIPGRAPRPNDASLDNSLARR
                     VLKTPMLSLEEALELTLQIRREQENGKS"
     gene            complement(1808..>2378)
                     /locus_tag="PKN92_13305"
     CDS             complement(1808..>2378)
                     /locus_tag="PKN92_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49816.1"
                     /translation="FLRYALDSWLPTFLELQGLGKGQAAYYSSIFDGAGLAGAILAGI
                     ALDQIFKGRWDRLCFVLSLGTLAGYTTVTFFGANPRALALCFGLVGFMLYGPDTLLCG
                     AGSVVVAGERNAVAVAGLVNGMGSIGPIIQEQLIGRLMEGRTPQESVRIANSLGLYVS
                     CLFVLLMALIFWQTSVTIERRKQEEEDSS"
BASE COUNT          547 a          689 c          609 g          533 t
ORIGIN      
        1 ttccttccac cacttcaggc agccgaaaac ccgggatatc cttaagccct tccgtcagat
       61 acgcagcgtt atcaatacgc cgctgattca gcatgtccag tttgcgcagc tgtacgctgc
      121 ccactgccgc ctggatatcc gtcaggcgga aattgtagcc tgtgtcatca aagtcctgcc
      181 accagaagcg tttgcctttg ggcagcttgc tttcgtccag cgaacagtat ttggtgctgg
      241 ctccgtatac gcgggtgcag tgggagcggt agagcgcaat cctttcgtat atatcggcat
      301 catccgtcgt aatgatgccg ccttccccca gagtggaaat atttttctgc tcatgaaagc
      361 tgaaacagcc taccttgccc agcgccccgg cttttcttcc tttataggcg ccgccgaccg
      421 tgtgacaggc atcttcgacc acagcaagac cgtgcttttc ggcaatagca ttgattgcat
      481 ccatatcgca gcattgaccg tagagatgaa caggtatgat tgctttggtt tccggagtga
      541 tagccgcttc tatctgcgcc ggatcaatat tccaggtgcc ttcttcaaca tccacaaagt
      601 cgatctgcgc gcccagcgtg accggagccg ccgccgtggc aatccaggtg agcggtgtag
      661 tgatgacccg atcccccggc ttaatgccga tgccgatcat ggcaagaaaa agtgccgctg
      721 ttccattact gacgcaacgt gcatgactca caccgcagta cgcggcaaat tctttttcaa
      781 aagcaataca ggcacttccg ctggtcggcg catttttacg cagcacctcc agcacggctg
      841 cttcgtcatc gggtccgtat tcattgccga attgaatcat cagatcatgt ttgatttcag
      901 gacttgccat tttcttgttc tctcctgatt tgaagggtta attccagtgc ttcttccaga
      961 gaaagcatgg gggtttttaa aacacgacgg gcaagactgt tgtccagaga ggcatcatta
     1021 ggcctcggtg cccgcccggg aattgcattg gaatcgacgg caataatttt tccctgcggc
     1081 agatccaggc gtgcggcaat gcgctgcgcc atctcatagc gatttaacct gctgcagccg
     1141 gacagatgca ggatgccggt aaagctgtgc tccgccagtt cccagaaagc gcggccgagg
     1201 gtgattacgt ctacaggcgt ccgtatttca ttcgccggga aggaggcttc tccgcccgat
     1261 tccagtgtgt gaatcatctt cgccagaaaa gagttgccct caccgagcac ggaaaagccg
     1321 agtaccagcg acaggcgggc tactacggaa tccaatacgg tggaaagaac tgtttcttcc
     1381 gcctgtactt ttgtttcggc ataataattg accggagacg gagcgtcact ttcccggtac
     1441 atcccttgaa tcccgtcgaa aatgctgtcg gtagaagtga aaatgagacg cgccccctga
     1501 tcacggcata acgtggagag ctgtgcggta acgcctgcat tcaccgcttt cgccgcttcc
     1561 tgattctgct ggcaccggtc aatatccgcc atggctgccg tatgaaaaac aacatccggc
     1621 cggatccttt taaaaagtct ttcaaggtct gcagtatcaa ggagttccaa cggatgcacc
     1681 tgaatcccgg caggtgccgg gagatccgcc ttccggcttg tgccgtgaag atcccactcc
     1741 ggcggacagt gccaggcaat actcccggcg acaaatccgc cgaatccggt caggagcgct
     1801 cgttttttca tgaagaatct tcctcctcct gttttcgccg ttcaatcgtc acgcttgttt
     1861 gccagaaaat cagcgccatc agtaagacaa agaggcagct cacataaagc ccgagggaat
     1921 ttgcaatgcg caccgattcc tgaggggtac gtccttccat gagccgaccg atcagctgtt
     1981 cctgaatgat cggaccgata ctgcccatgc cgttcaccag cccggcaact gccacggcgt
     2041 tgcgctcccc ggctactact acagacccgg cgccgcagag aagggtgtca ggcccgtaca
     2101 gcataaaacc caccaacccg aaacagagcg caagcgcccg gggatttgcg ccgaaaaagg
     2161 taaccgttgt atagcccgca agcgtgccca gactcagcac aaaacagaga cggtcccatc
     2221 ggcctttaaa aatctgatcc agagcgattc cggcaagaat ggcaccggca agacctgcgc
     2281 cgtcaaagat ggaagaataa taggcggcct gccctttacc cagtccctgc agttcgagaa
     2341 aggtgggcag ccaggagtcc agcgcatagc gcagaaat
//
LOCUS       DAOPWB010000244         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_47395_length_2373_cov_5.213115, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000244 DAOPWB010000000
VERSION     DAOPWB010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47395_length_2373_cov_5.213115"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKN92_13310"
     CDS             <1..339
                     /locus_tag="PKN92_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNZ49817.1"
                     /translation="LGDPVEDFLCPDFDVNRRGDWTKDAAGRRSARLIKNLVSPRPVI
                     LKDKCTRCGTCVKVCPIDPPAVDFLQEDKSRPPEYDYKRCIRCYCCQEMCPDSATVVE
                     TPLLGRLIHR"
     gene            complement(340..>2373)
                     /locus_tag="PKN92_13315"
     CDS             complement(340..>2373)
                     /locus_tag="PKN92_13315"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNZ49818.1"
                     /translation="LIDLSQPLPEWAAFYDAAPCGAADSAEVPGLPVCFEHADWPQMR
                     LERPGEVWDWSAYAGVGLSLYNPTDEAFALCFRLDNPGTDGTSLSNRLRTTILPGQHL
                     DFTMRFVRKDGPGLWGMRGLPGTGQYGEGTALDLTRISSLQIFLPNPERSRTLILKRW
                     FLFGHASDAEEEVPLPFIDRFGQYKHADWPGKVTGEEELRQRLAEEREQRMRSAAVSG
                     YDRFGGWADGPRREATGWFRTEKIDGQWWLITPEGSLFFSLGADCVGYGERTFVEQRE
                     SWFDWLPGETETPFCQIYTRNSGAHSYAEPIGGEGRTFSFYQANLMRKYGDSWQDDWR
                     SDAVARLRCWGFNTIGNWSHWDILRQSEMPFVVSSGFWGIRLIERSRGYWSKMQDVYD
                     ESFPRQVEKAVAGLTEVWKNNPFCIGYFFDNELAWEGVQDGVLSSGAEQPARQAFIAW
                     LQERYSSPDQLNRAWAADFGSWDDVQGTGAPTQAFTLDMSDFLHHFALRYFTVIQETV
                     KRHDPNHLYLGCRFASAPPEVVRACSETADVLSFNLYYNTLPANFLKNTALQDKPVII
                     GEFHFGALDRGMFHTGLVGAKDQKARGRLYRDYVESVLAHPSFIGCHWFQWVDEPVTG
                     RWMDGENYNLGFVDVTDSPYPELTEAAKEVHSRAYTFRASFRNESNTFNEAAP"
BASE COUNT          514 a          690 c          653 g          516 t
ORIGIN      
        1 ctcggtgatc ctgtggagga ctttctctgt ccggactttg atgtcaaccg cagaggcgac
       61 tggactaaag atgcggcagg gcgccgcagt gcccgactca tcaagaatct cgtgtcgccg
      121 cgccccgtaa tcctcaaaga caaatgtacc cgctgcggta cctgcgtgaa ggtctgcccg
      181 attgaccctc ctgctgttga ctttctgcag gaagacaaaa gccgcccgcc cgaatatgac
      241 tacaaacgct gtatccgctg ctactgctgc caggagatgt gtcccgacag tgctaccgtg
      301 gtcgagacgc ccttgctcgg acggcttatt caccgctgat cagggagcgg cttcgttgaa
      361 cgtattgctt tcgttacgga agctggcgcg gaatgtgtat gcccggctgt gcacttcttt
      421 tgctgcttct gtcagctctg ggtaaggtga atcggtcacg tccacaaagc ccagattgta
      481 attctcgccg tccatccatc gccccgtgac aggttcgtcc acccattgaa accaatggca
      541 gccgataaaa gagggatgag caagcacgct ttccacatag tccctgtaga ggcggccgcg
      601 agccttctgg tcttttgctc cgaccagtcc tgtatggaac atgccccggt ccagtgcgcc
      661 gaaatgaaat tctccgatga taaccggttt gtcctgcagt gctgtgtttt tgaggaaatt
      721 tgcaggcagc gtattatagt aaaggttaaa tgagagaaca tcggcggttt ctgagcaggc
      781 acgaaccact tcgggggggg ccgaagcgaa gcggcatccg agatagaggt ggttgggatc
      841 atggcgcttc actgtttcct gaatcaccgt gaaatagcgc agcgcaaaat ggtggagaaa
      901 atcagacatg tccagcgtaa atgcctgggt cggcgcacct gtgccctgta catcgtccca
      961 gctcccgaaa tcagcagccc acgcccggtt taactgatcc gggctgctgt accgctcctg
     1021 gagccatgca atgaaagcct gccgtgccgg ttgctcggcg ccgcttgaga gaacaccgtc
     1081 ctgcacccct tcccaggcga gttcattgtc aaaaaaatag ccgatgcaaa aggggttgtt
     1141 tttccagact tcagtgagac ccgctaccgc cttttcaacc tgacggggaa aagactcgtc
     1201 atagacatcc tgcattttgc tccagtagcc tcgggaacgc tcgatcagcc tgatacccca
     1261 gaagccggac gaaacgacaa aaggcatttc agactggcgc agaatgtccc agtggctcca
     1321 gttgcctatc gtgttgaatc cccagcatct taggcgggca acagcatcgc tgcgccagtc
     1381 gtcctgccag ctgtctccat atttgcgcat cagattggcc tgataaaaac tgaaggtgcg
     1441 gccttctccg cctataggtt cggcgtaact gtgcgctcct gagtttcttg tgtaaatctg
     1501 gcagaagggc gtttccgttt caccgggaag ccagtcgaac cagctttccc gctgctccac
     1561 gaaggtccgt tctccatagc cgacacaatc agcgcccagt gaaaagaaaa gactgccttc
     1621 cggtgtgatg agccaccact gcccgtcgat tttttcggtg cggaaccatc cggtggcttc
     1681 ccggcgggga ccgtccgccc atccgccgaa acggtcatat ccggaaacag cagcgctgcg
     1741 catccgctgt tctctctctt ctgcaaggcg ctgccgcagt tcttcttcac ctgtgacttt
     1801 gcctggccag tcggcatgct tgtattgtcc gaaacggtcg ataaaaggca gcggaacttc
     1861 ttcttccgca tcggaagcat gaccgaaaag aaaccagcgc ttcagtatca gcgtccggct
     1921 gcgttccgga ttgggaagga aaatctgcag cgatgagatg cgtgtcagat ccagcgctgt
     1981 gccttcgcca tattgacccg tgccaggaag gccgcgcatg ccccagagtc ccgggccgtc
     2041 ttttctgaca aagcgcatag taaaatccag atgctgaccg gggagaatgg tcgtacgaag
     2101 cctgttggac agacttgtac cgtcggtgcc ggggttatcc aggcgaaaac agagggcgaa
     2161 agcctcatcg gtggggttgt acagggatag ccccacgcct gcataagcgc tccaatccca
     2221 cacttcgccc ggtcgctcca gtcgcatctg tggccagtcc gcatgctcga aacagaccgg
     2281 cagcccggga acttcggcac tgtccgcagc tccgcagggg gctgcatcat aaaaagccgc
     2341 ccactcggga agcggttgag agaggtcaat cag
//
LOCUS       DAOPWB010000245         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_49390_length_2306_cov_5.727232, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000245 DAOPWB010000000
VERSION     DAOPWB010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49390_length_2306_cov_5.727232"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PKN92_13320"
     CDS             complement(<1..959)
                     /locus_tag="PKN92_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49819.1"
                     /translation="MKNKLVIGMPAGSLADVNRGGNLVTLLKAAGFPTQGYEKGGPTT
                     FPLHSMLFGWDGRPQEFGAQLALHEVDIAIGGDDWIAERLLEYKHEYGKNITLSKALS
                     LGRGKVRLVIINAPRPEGQSFDAWLTQLLTEQKIIRAVSEMPYLFIQWFQKKRAALGF
                     GEEWDCYSIQKYNTPPKIEKGLVVYESWGKTEAKVLHGSVDFGLEITQTGSAIRNYGL
                     EISEEIMQSEAGVWAAEGLKNDPEKYELARMFLLNLYGAVFAENKVLVLFNARKDQVP
                     AMIDYLDKHRLFGDEPTMNEGINFTEFNIQVRTADPALPLAQVR"
     gene            complement(1103..1915)
                     /locus_tag="PKN92_13325"
     CDS             complement(1103..1915)
                     /locus_tag="PKN92_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNZ49820.1"
                     /translation="MKFGICNEIFKEWNDLARTINFVKETGYDGLEIAPFTLAPYVTD
                     IPASTRSEIVRLAAAADLDILGIHWVFVGPEGVFLTHPDAEVRRFTTQYLIDLVNFCG
                     DVGGKVLIFGSPKQRNVGRDVTYNQAFDYAREIFEAAMPACEARDVTICMEQLTHWET
                     NFCHTPEQTVELIDAVNHPHFQLLLDTKAMSYLEEDRATLIRKYGKYLRHYHANDTNF
                     HGPGWGEVDFAPIFDALRDINYDRYVSVEVFRFEAGPEAIAQKSLEYMKQFV"
     gene            complement(1963..>2306)
                     /locus_tag="PKN92_13330"
     CDS             complement(1963..>2306)
                     /locus_tag="PKN92_13330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNZ49821.1"
                     /translation="KAIGEVIRQCTRDYVDVGCRYGGDEFTVILPETDLVHAKNIAER
                     IRVSFEARAFDQCTLSIGLMTNNKTAPDKGASAESYIRFADEMMYEAKRSGGNRVCIY
                     DGSPNVAGPDA"
BASE COUNT          520 a          631 c          598 g          557 t
ORIGIN      
        1 cgcacctgtg caagaggcag tgcgggatca gcagtgcgca cctgaatgtt gaactcagta
       61 aaattaatgc cttcgttcat agtgggctca tcgccgaaaa ggcggtgttt atccagatag
      121 tcgatcatgg cgggcacctg atctttgcgg gcattgaaca aaaccagcac cttgttttcg
      181 gcaaagacag cgccgtaaag attcagcaga aacatgcgtg caagttcata tttttccggg
      241 tcgtttttca gaccttccgc cgcccagacg cctgcttcgg actgcatgat ttcttcactg
      301 atttccagcc cgtaattccg gatggcgctg ccggtctggg tgatttccag cccgaaatca
      361 acactgccgt ggagtacttt cgcctcggtc tttccccacg attcataaac gaccagcccc
      421 ttttcgatct tcggaggcgt attgtatttt tgaatggagt agcagtccca ttcctcacca
      481 aagcccagtg ccgcgcgttt cttctgaaac cattgaatga agagataggg catttccgaa
      541 acggcacgga ttatcttctg ctctgtcagc agttgtgtca gccaggcatc aaaactctgc
      601 ccttccggac ggggagcgtt gataatgaca agccgtactt tgccccggcc cagcgacagt
      661 gctttggaaa gcgtgatgtt tttcccgtat tcgtgcttgt attccagaag ccgttcggca
      721 atccagtcat cgccgccgat ggcaatgtcg acttcatgca gcgccagctg cgcgccgaat
      781 tcctgagggc gtccgtccca gccgaaaagc atggaatgga gcgggaaggt ggtaggcccg
      841 cctttttcgt atccttgcgt gggaaagccg gccgctttaa gcagggtaac aagattaccg
      901 ccccgattga catctgccag cgatccggca ggcataccga taacaagttt gtttttcatg
      961 aaatgctcct gcagcgggaa aaagttgccc gctgaaaaat gagttgattt acgggagagc
     1021 cgtttctgcc ttggcagaag agtgtcccgg tttatatcca cgcactcccg aagattgcag
     1081 cagtgtatca cctgaagggg gatcagacaa actgcttcat atattccaga ctcttttggg
     1141 cgatcgcttc cggccccgcc tcaaaacgaa atacttccac agacacataa cggtcgtaat
     1201 taatgtctct gagcgcatcg aagataggag cgaagtcgac ctcgccccag cccggcccgt
     1261 ggaaatttgt gtcgttcgca tggtaatgac gcaggtattt cccgtatttc cggatcagtg
     1321 tcgcccgatc ctcttccaga taggacatgg ctttggtgtc cagaagcagc tgaaaatggg
     1381 gatgattcac cgcatcgatg agctctaccg tctgctcggg cgtgtggcag aaattggttt
     1441 cccagtgggt cagctgttcc atgcagattg taacgtcacg cgcttcacag gcgggcatgg
     1501 cagcttcgaa aatttcgcgg gcgtaatcga aggcctggtt gtaagtaaca tcccggccta
     1561 cattgcgctg cttgggtgag ccgaaaatca gcacttttcc gcccacatct ccgcagaaat
     1621 tgaccagatc aatgagatac tgtgtggtaa aacggcggac ttcagcgtcg ggatgggtga
     1681 gaaaaacacc ctccgggccg acaaaaaccc aatgaatccc cagaatatca agatcggcgg
     1741 ccgctgccag ccgtacaatt tccgaccgcg tcgatgcggg gatgtcggtg acatagggcg
     1801 ccagtgtgaa gggagcgatt tcgagaccgt cgtaccctgt ttccttcaca aaattgatgg
     1861 tgcgcgccag atcgttccac tctttaaata tttcgttgca aattccgaat ttcataaaac
     1921 ctccggtatg tttagggaag cgctgcggct ctgccggcac tgtcatgcat cagggcctgc
     1981 aacgtttggg ctgccgtcgt aaatgcaaac ccggtttccg cccgaccgct tcgcttcgta
     2041 catcatctca tccgcaaaac ggatgtagga ttccgcactc gcccctttgt cgggcgccgt
     2101 tttattgttc gtcatcagtc cgatactgag cgtacattga tcaaaagccc gtgcttcaaa
     2161 ggaaacccgt atgcgctcgg caatgttctt cgcatgaacc agatccgttt ccgggaggat
     2221 caccgtgaat tcatcgcctc cgtaacggca gcccacgtcc acatagtcac gggtgcactg
     2281 gcggatcacc tcgccgattg ccttga
//
LOCUS       DAOPWB010000246         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_53787_length_2181_cov_4.247883, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000246 DAOPWB010000000
VERSION     DAOPWB010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53787_length_2181_cov_4.247883"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKN92_13335"
     CDS             complement(<1..296)
                     /locus_tag="PKN92_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49822.1"
                     /translation="MALRCLILSALLLALRCPAAPPEQVFTVRIKASVEGVGLSARKA
                     ALEKAEREALAEAIESIIESADLSRYRAMLDEAPRYIRQTRVLHTETVGQNTDL"
     gene            complement(320..1009)
                     /locus_tag="PKN92_13340"
     CDS             complement(320..1009)
                     /locus_tag="PKN92_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNZ49823.1"
                     /translation="MFFFHPADLLMIPAILLSLWAQMRVKGAYAKYAAVRTQSGLTGA
                     EVASRILNGARIDNVAIEPIPGELTDHYDSEKKKLRLSQSIHSGNSIAAVGIAAHEAG
                     HALQDAQNYGPMRLRHSIYPLASLGSSLAFPLIFIGLIFNFSFAGALIQAGILLYTAA
                     VAFTLLTLPVEFDASRRAVAILASTGQFSPDEMRGVRRVLSAAALTYVAAAAAAALQL
                     LRLILIAQRRR"
     gene            complement(1018..1746)
                     /locus_tag="PKN92_13345"
     CDS             complement(1018..1746)
                     /locus_tag="PKN92_13345"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNZ49824.1"
                     /translation="MNRSSGQKPQSGGTPSQPKKADKEAGLFSKLGNRFNTALQSSRG
                     EDAAQPAAGRPVPASAPAPASAAQASVRKPSEETASIRTKGNVASMKMYIPEGTVVQG
                     SIKGKTETEIHGRIEGDVSVEASLYLGKNAVITGNVHAASCQVDGKVEGSMNCTDELV
                     LSAGSALAADASAGKQVRISGKIEGNVTTPGTLRIEAGGVVNGDVQARVFSMSEGAVL
                     NGRCTMRTAQQETLFKETEKPNKG"
BASE COUNT          474 a          582 c          578 g          547 t
ORIGIN      
        1 tcaaggtctg tattctgccc gactgtttca gtgtggagga cgcgggtctg ccgtatatag
       61 cggggcgctt catccagcat ggcgcgatag cggctcaggt ctgctgattc gataatggat
      121 tctatcgctt cggccagtgc ttcccgctct gctttttcca gagccgcttt gcgtgcggaa
      181 aggcccaccc cttcgacaga ggcttttatg cggacagtaa agacctgttc gggcggagcg
      241 gcgggacagc gcagcgccag cagcagtgcg gacaatataa gacagcgcaa tgccataggc
      301 gtgcttcctg cagataagtt catcggcgcc tttgtgcgat aagaatcagg cgaagcagct
      361 gcagggcagc agccgccgct gcagcgacat aggtaagtgc cgctgcactc agcaccctgc
      421 gaacccctct catttcgtcc ggagaaaact gccctgtgga tgcaagaatg gcaacagcac
      481 ggcggctcgc atcaaactca acgggcaacg tgagcagcgt aaaggcaacc gctgccgtgt
      541 acagcagtat tccggcctga atcagtgccc ctgcaaatga aaaattaaaa ataagcccga
      601 taaaaatcag cggaaaagca agagaacttc ccagcgatgc cagtggataa atactgtggc
      661 gcagccgcat aggaccgtag ttctgtgcgt cctgaagcgc gtgccctgct tcatgcgccg
      721 caatgcccac ggcggcgatg gagttgccgg agtggatgct ctgggagagg cgcagctttt
      781 tcttttcaga atcgtaatga tccgtcagtt cgcccgggat cggttcaata gcgacattgt
      841 cgattcgtgc gccattgagg atacgggaag caacttcagc tccggtcagc cccgattgtg
      901 tccgcacagc ggcgtatttg gcgtaggcgc ctttcacacg catctgtgcc cacagtgaaa
      961 gcaaaattgc cgggatcatc aacagatctg cagggtgaaa aaagaacatg gttgtgctta
     1021 gcctttattc ggtttttccg tttccttaaa gagcgtttcc tgctgtgcgg tacgcatggt
     1081 gcagcgaccg ttcagcacgg caccctcact catggagaaa acgcgcgcct gaacatctcc
     1141 gttaacaacg ccgcccgctt cgatgcgcaa cgtgccgggg gtcgttacgt tgccttcaat
     1201 ttttccggaa atacgtacct gctttccggc tgaggcatca gcggcaagag cactgcccgc
     1261 actgagaacc agctcatccg tgcagttcat gctgccttca actttgccgt ccacctgaca
     1321 ggatgcggca tgaacatttc cggtaatcac cgcattttta ccgagataca gagacgcctc
     1381 aacggaaacg tcgccttcaa tgcgcccgtg aatttctgtt tctgtttttc ccttgattga
     1441 gccctgcact actgtacctt cggggatata cattttcata gatgccacgt ttccttttgt
     1501 acggatggaa gctgtttctt ctgaaggttt tctgactgat gcctgagcgg cggacgccgg
     1561 tgccggtgcg cttgccggga caggccggcc cgctgcgggc tgtgccgcgt cttcgccgcg
     1621 actgctttga agcgctgtgt taaaacggtt tcctaatttt gagaacaaac cagcttcttt
     1681 gtctgctttt ttaggctgcg acggcgtacc gccggactgg ggcttctgac cggatgatcg
     1741 attcataggg tgtatcctca taccccactg gggcgttcat ttaggcaatt gcaaaaactc
     1801 gggacacgtc aatcaatgca tgtgccctat agtctactca caagtccgta tcaggttcaa
     1861 cagacccggt ttctactgcc tgcaggggag cgccgactgc tttacgtccg ctcattatca
     1921 agttcaaaat ttaagtgttc cgtgaataaa taacaccttt cgtgtgtata atgtataata
     1981 taatcagtga cgtgaaagaa gccatgtagt ttttttttac cggcagtttt tgcctttcat
     2041 aactattata gtttcactgg ttgaatcacc ttgttaaaag gagagggctt tcttatggtt
     2101 ttcttgcgca taacctgtag tttcgttgtt ttggcgggtc tctgcgctgc agcatatgcc
     2161 catgaaccgg tgcccggacc c
//
LOCUS       DAOPWB010000247         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_54981_length_2149_cov_5.637058, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000247 DAOPWB010000000
VERSION     DAOPWB010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54981_length_2149_cov_5.637058"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="PKN92_13350"
     CDS             complement(<1..1750)
                     /locus_tag="PKN92_13350"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNZ49825.1"
                     /translation="MNRILLTFLTAGLFLTLQAGAALTLYVAPDGEDLFSGSAASVNG
                     DRTDGPLKSLAGARDRIRVLRSEKGLPSGGIRVLIAPGIYRLEESFVLDAEDTGMPGC
                     PIVYEAQEKNTVHISGGRAISNFVPVKDQAVLDRLDPAAREHVLSADLKALGISSFGT
                     PEGGAALYFRNTPMTLSRWPNEGFTSIVDLVVQDGHSIHGVPGSMTGLFTYEGDRPAR
                     WVDEKDGWLHGYWFWDWSDERKPIAAIHPELSQIRVAEPAHGYGYRKGQWYYAYNLLS
                     EIDEPGEWYLDRGEGILYFWPPETPEKEDACFSVLPKLIEIRECSNILLKGLSLEHAR
                     DLALEVTDGAGVQVMGCTVCNCGGDAMSLSGAQGHLVFGCHIYQVGGKGISLTGGDRT
                     TLTPAGHRAENNLITDFSQWYRMYRAGINLIGVGNCARHNHIHHAPHMGIFFSGNDHL
                     LEYNLIHHVCLESNDAGAIYAGRNWTMRGNLIRFNHLHDIQGFQDKGCVGIYLDDMFA
                     SADMISNLFERVTRAAFIGGGRDCDVVNNVFIDCVPALHVDARALGWAAYHADEWVAE
                     GKEKGTLSGIRYQEPPY"
BASE COUNT          484 a          614 c          577 g          474 t
ORIGIN      
        1 tgtagggcgg ctcctgataa cggatgcctg aaagagtgcc cttctctttt ccttccgcga
       61 cccactcgtc tgcatggtag gcagcccagc ccagcgcccg tgcatccaca tgaagcgcag
      121 gaacgcaatc gatgaacaca ttgttcacaa cgtcgcagtc tctgccgccg ccgataaaag
      181 cagcccgggt tacccgctca aaaagattgc tgatcatatc cgccgatgcg aacatatcat
      241 ccagataaat gccgacacag cctttgtcct gaaagccctg aatgtcatgg agatggttga
      301 accggatcag attgccgcgc atggtccagt tccgcccggc ataaatcgca ccggcatcgt
      361 tggattccag gcacacatga tggatcaggt tatattccag cagatggtca ttgccggaaa
      421 agaaaatgcc catatgcggc gcatggtgga tatgattgtg ccgggcgcag ttgcccacac
      481 cgatgagatt gatcccggcg cggtacatgc ggtaccactg gctgaaatcg gtgataagat
      541 tgttctcggc gcggtgtcct gcaggagtaa gcgtggtacg atcgccgccg gtcagactga
      601 tccccttgcc gcccacctga tagatatggc atccgaaaac gagatgtccc tgtgcgccgg
      661 aaagcgacat ggcatcaccg ccgcagttgc acactgtaca gcccataacc tgcacccctg
      721 ccccgtcggt cacttccagt gcaagatccc gggcatgctc caggctcagc cctttcagaa
      781 ggatattgct gcattcccga atctcgatca gcttgggcag cactgaaaaa caggcatctt
      841 ctttttccgg tgtttccggc ggccagaagt agagaatacc ctccccgcga tccagatacc
      901 attcgccggg ctcatcgatt tccgacagca ggttgtaggc gtaataccac tgcccttttc
      961 tgtagccgta gccgtgggcg ggctcggcaa cacggatctg tgagagctcc ggatggatgg
     1021 cggcaatggg ctttcgttcg tccgaccagt cccagaacca gtagccgtgc agccagccgt
     1081 ctttttcatc gacccaccgg gcaggccggt cgccttcata ggtgaaaagc ccggtcatgc
     1141 ttcccggtac gccatgaatt gaatgaccgt cctgcaccac gagatcgaca atggacgtga
     1201 agccttcatt aggccaccgg gacagagtca tcggggtatt gcgaaaataa agggctgctc
     1261 cgccttcagg cgttccgaag gaactgatac ccagcgcctt cagatctgcc gacagcacat
     1321 gttcccgggc tgcaggatcc agccggtcca gaacagcctg atcctttacc ggaacaaaat
     1381 tgctgatggc tcgaccgccg ctgatatgca ccgtgttttt ttcctgtgcc tcataaacga
     1441 tagggcagcc gggcataccg gtatcttccg catccagaac aaagctttcc tcaagcctgt
     1501 aaatacccgg agcaatcaga accctgatgc cgcccgaagg caatcccttt tcgctgcgga
     1561 gaacacggat gcggtcgcgg gctccggcaa ggcttttcag agggccgtcg gtacgatcgc
     1621 cgttgacgga cgcagcggat ccggagaaga gatcttcacc gtccggcgcc acgtaaagcg
     1681 tgagagcagc ccctgcctga agtgtgagaa ataatccagc ggtaaggaag gtcagcagta
     1741 tccggttcat ggtatatccc tttcagttgc ctgttgcgta ttatagcaat gtcctgggac
     1801 atactcccat aaagatagtc ctcacctgcc tcttgccaat acggagattg gcgttcccag
     1861 gctggcggat tttatgtttt gagtcagcgt attttggtgt gaagcccata taagtctcag
     1921 tagaaattca tgtccattcg tgttcattcg gggttcttcc ctatgacaag gagtcgattg
     1981 gtgctcgcag aagatgcggc gatggcttta taagggtggg ggcatgcgca gaacgcacag
     2041 tccactgtgt ccactctgtc cacccacttt ggtgtggcat gcagtcctgt aaggcaaaga
     2101 cgcccataac ctgattaaga ttcctgcctt cgcaggaatg acggtggaa
//
LOCUS       DAOPWB010000248         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_55357_length_2140_cov_6.458513, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000248 DAOPWB010000000
VERSION     DAOPWB010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55357_length_2140_cov_6.458513"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKN92_13355"
     CDS             <1..964
                     /locus_tag="PKN92_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNZ49826.1"
                     /translation="YRKDVPQDGRIDRDKTSAGRPMRVSAVPTVRGEKIVIRILGSSR
                     DLYQLDILGFPERIVHGVQRIITRPQGTLLLTGPSSSGKSTTIYAALHEILHLQKHTI
                     NIVSIEDPVEFAMDRIAQIQVNPHADLTFTTALRSILRQDPEVIVVGEIRDFETAKMA
                     IQAGLTGHLVISTIHSGTAAGVFTRLIDMGIEPFLVASSITGVLAQRLIRLNCPDCTE
                     EYTPDPLMMHYFGLTAESGPFYKGAGCKECQNIGYRSRAGIGEFLEVDNTLAEQLLRH
                     PTTSQIHEIAVSKGMIPLAKDGLDKARLGITTIEELYRVLPELS"
     gene            complement(967..2046)
                     /locus_tag="PKN92_13360"
     CDS             complement(967..2046)
                     /locus_tag="PKN92_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002759436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNZ49827.1"
                     /translation="MYNGKTVLVTGGAGFVGSHLALAFKRRFPAARVIVLDNLKRRGS
                     ELNIKRLIRGGVEFLHGDVRNKEDLTGISVLDLLIECSAEPSVLAGYSTSADYVIGSN
                     LMGAVHCLEVAKAHQAAFIFLSTSRVYPVKYLNDLSLVEKDTRFELAESQPVAGVSPA
                     GIDHTFPLEGARSLYGATKLSAELLIQEYRDMYGLQTVINRCGVIAGPGQMGRVDQGI
                     LAFWLAAHIYEKPLSYIGFGGMGKQVRDMLHIEDLSELILTQALSLEKADGKARNAGG
                     GREGSVSLFELTQMCEAFTGKRIPVTAVPETRPADIPLYITDNKAISDFYGWRPTRGI
                     TRLVEETAQWIYDNKEMLSPIFCAE"
BASE COUNT          545 a          567 c          486 g          542 t
ORIGIN      
        1 ctatcgcaaa gacgttccgc aagacggtcg aattgatcgt gataaaacat ctgcaggcag
       61 gcccatgcgt gtatctgctg tgcctaccgt ccggggagag aaaattgtta ttcgtatttt
      121 aggctcgtcc cgggaccttt atcaattgga cattcttggt tttccagaga gaattgttca
      181 cggcgtacag agaatcatca cacgcccaca ggggacactc ctgctgaccg gccccagttc
      241 aagcggcaaa tcaacgacta tttatgcagc gcttcacgaa attcttcacc ttcagaaaca
      301 tactatcaat attgtttcca ttgaagaccc tgtcgagttt gccatggatc ggattgcaca
      361 aatacaagtc aacccccatg ccgatctcac ttttaccacc gcactccgct ctatattgcg
      421 ccaggatcct gaagtaattg tagtcggaga aattcgcgat tttgaaacag caaagatggc
      481 tattcaggca ggtcttacag gtcatttggt tatcagcacg attcacagcg gtactgcagc
      541 aggcgttttt actcgtctga ttgatatggg catcgagccg tttctggtgg catcttccat
      601 cactggcgtg ctggcgcaac gtctgatccg gcttaattgt ccggattgca ctgaggagta
      661 cacgcctgat cccctaatga tgcactattt cgggttaacc gcggaatccg gtccctttta
      721 caagggagcc ggctgcaagg aatgccagaa catcggctat agaagtcgtg ccggaatcgg
      781 ggaatttctt gaagttgaca ataccctcgc agaacagctg ctccgccacc cgactacctc
      841 tcagatacat gagattgccg tttctaaagg aatgattcct ctggcaaaag acggtctgga
      901 taaagcaaga ctgggcatta ccaccattga agaactgtat cgtgtgcttc cggagctcag
      961 ctgattttac tctgcgcaga atatcgggct aagcatctct ttgttgtcat aaatccattg
     1021 tgctgtttct tccaccagcc tggtaatgcc tcgggtagga cgccatccgt aaaaatcgga
     1081 tatcgctttg ttgtccgtaa tgtacagagg aatgtctgca ggtcgggttt caggaaccgc
     1141 tgttacaggt attcttttgc ccgtaaaagc ttcacacatt tgcgtaagtt caaataaaga
     1201 cacgctgcct tctctgcccc cgccagcatt gcgtgccttt ccgtcagctt tctcaagaga
     1261 caatgcctgt gtcaggatga gttccgacaa atcttctata tgcagcatat ccctaacctg
     1321 ttttcccatg cctccgaagc cgatgtacga taagggcttc tcataaatat gggctgctag
     1381 ccagaacgca agaatgccct gatccacccg acccatctgc cccggtccgg caatcacacc
     1441 gcagcgattt attaccgttt gcaaaccata catgtcgcgg tattcctgaa tcagaagttc
     1501 ggcgctcaat ttggtcgctc cgtaaagaga gcgggctccc tccaggggga atgtgtggtc
     1561 aataccggcc ggggacacgc ctgctaccgg ttgtgattcg gccaactcaa aacgcgtatc
     1621 tttttccacg agtgacagat cattcaggta cttgacagga taaacacggc ttgtagagag
     1681 gaaaataaaa gcagcctggt gcgctttagc cacctcaaga caatgcactg ctcccatcag
     1741 attactgccg atcacataat cagccgatgt gctgtagcct gccaacacag aaggttccgc
     1801 actgcattca ataagcagat ccaatacgga tataccagtc agatcttcct tattccggac
     1861 atcgccgtgt agaaattcca caccgccccg gataagcctt tttatattca gttcacttcc
     1921 ccgccgtttc agattatcca gcactattac ccgtgcggcc ggaaaacgcc ttttaaaagc
     1981 aagagcaaga tgagatccaa cgaaaccggc accgccggtt acaagaacag tttttccgtt
     2041 atacatgact catcctttca tcttaataaa aaccgggcag gagacatggt agcatatctc
     2101 ctgcccggca aagcgttcaa gggtaaataa acaggtggtt
//
LOCUS       DAOPWB010000249         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_55405_length_2139_cov_4.566699, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000249 DAOPWB010000000
VERSION     DAOPWB010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55405_length_2139_cov_4.566699"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKN92_13365"
     CDS             complement(<1..645)
                     /locus_tag="PKN92_13365"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNZ49828.1"
                     /translation="MDNKESLSSPFIPWLAACCTVFVSSACIMVLELTAGRIIARHIG
                     VSLYTWTAVIGVIMLGMALGNHLGGRFADRYVPSRMLALLFLSAAGACFLILPLNSLA
                     GMLHFLGGAAWPLRIFLHVSLVFLVPALLLGMIPPVVVKMALDLHRKAGRTVGSIFAW
                     GVGGSLLGTFITGYYLVMVWNASTILFASGGGLGILGAAYSLHSLRHRSGGRTSR"
     gene            complement(761..1738)
                     /locus_tag="PKN92_13370"
     CDS             complement(761..1738)
                     /locus_tag="PKN92_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49829.1"
                     /translation="MKKVALIILAVLVVLLVVAAGGGAIAYKKFNSEYGLAASPVLHH
                     DAICEADTRIRLVAAPLLVAPFVEQYLPENMEIPTGPFSLQSVLDRVLPREVAALIKT
                     DMNTRKAQLTLFANEQRLGPYLEKILNDSAFLAKIKQISWTSNGFELPERGSLSARGT
                     LTVPETVESELFERWPTRVRETPAAVQGGHLLELVIDNRNGDILALAAAGVSASGQDW
                     ATFRKSQEAEMAMGVIQAIEVARITADLVDPDSAAFDLRIEADEKGGPGLQFLLSGLA
                     IPFATDYLKNEMGLALTGDLKWNAAEKAITGRYTLSGLKAVLRQQLAKK"
BASE COUNT          515 a          586 c          577 g          461 t
ORIGIN      
        1 acgggaagtc cttcccccgg aacggtgccg cagactgtgc aggctgtatg ccgcgccaag
       61 aatacccagt ccgccgcctg atgcaaaaag aatggtggag gcattccaga ccataaccag
      121 ataataaccg gttataaacg tgcccagcag acttcccccg acaccccagg caaaaatact
      181 gcccaccgtg cgcccggctt tccgatgaag atcaagtgcc attttgacca ccacaggagg
      241 gatcatgccg agaagcagag cgggtaccag aaaaacgaga gacacgtgaa ggaagattcg
      301 caaaggccag gcggcaccgc cgagaaaatg cagcatgccc gccagactgt tgagaggcag
      361 gatcagaaaa catgcccctg cagcactcag aaagagaagt gccagcatcc gcgacggcac
      421 ataacggtcg gcaaaacgtc cgccgagatg gttgcccagt gccatgccga gcatgatcac
      481 accgatcact gccgtccagg tatagaggga cacaccgata tgccgggcga ttatccttcc
      541 tgccgtcagt tccagcacca tgatgcatgc actggataca aatacggtgc agcaggctgc
      601 cagccacggg atgaagggag aggaaagaga ttctttattg tccatgactg actttctgca
      661 aggcttataa tgccggaaaa actgaaactg cacatcacct gcctgccggg gctggaaagc
      721 tgcccggcag agccgtcagg ggcattccgg agtccgcctg ttactttttg gcgagttgct
      781 gacggagtac tgctttcaga ccactcaggg tataacggcc tgtgatcgct ttttccgccg
      841 cattccattt aagatcgccg gtgagagcca gtcccatctc atttttcaga taatcggtgg
      901 cgaagggaat ggcaagtccg gatagcagaa actgcaggcc ggggcctcct ttttcatcgg
      961 cttcaatgcg caggtcgaaa gcggcgctgt ccggatcaac cagatctgcc gtaatccgtg
     1021 ccacctcgat ggcttgtata acacccattg ccatttccgc ttcctggctt ttgcggaagg
     1081 tagcccagtc ctggcctgaa gcagacacac cagccgctgc cagcgccaga atatcgccgt
     1141 ttcggttgtc gatgaccagt tccagcagat gaccgccctg aacagccgcc ggtgtctcac
     1201 ggactcgggt cggccaccgt tcgaagagtt cgctttccac cgtttccggt acggtcagtg
     1261 tgcccctggc ggaaagggaa ccccgttcgg gcaattcaaa accattggaa gtccagctga
     1321 tctgtttaat tttagccagg aaagcgctgt cattaaggat tttttccaga tagggcccca
     1381 ggcgctgctc gttggcaaaa agagtcagtt gcgctttgcg ggtattcatg tcggtcttaa
     1441 tcagggctgc cacttcccgc gggagaacac gatcaagaac agactgcagc gaaaagggac
     1501 ctgtcggaat ttccatgttt tcaggcagat actgctcaac aaagggtgcg acaagcaggg
     1561 gagcggcaac cagccggatg cgggtgtcgg cttcgcaaat ggcgtcatga tgcagtaccg
     1621 gtgatgcagc gagcccgtat tcactgttga attttttata ggcaatggcg ccgcctccgg
     1681 cagccaccac cagcaggaca acgagtacgg caagaataat cagagctact tttttcatct
     1741 gtttttcctt atgcctcaac cggacttttg tgcccggcgg gagaatgcag aaaaggacct
     1801 tatgacgtat gcaaagcgca gtaaacagcc ttcagtatac tgaaagggga aagagtgaat
     1861 ccagaaaata ccggtccctt tttctgcggc gcggcttttt tctattgtgc aggacataat
     1921 gccgcaccgt ttttgtgccc gatcgtcctg caaagcgggg ttgatttata ccacctttaa
     1981 taggtattct tgaaccggaa tccaattcat tccacagggc gggtctgcac tgcacgggtc
     2041 cttccctgtg cccttatcaa tgcgcaacga aaaggaactg ctcatgggac ttattgccgg
     2101 tcttttcttc cattggctcg gtggtttggc atctgccag
//
LOCUS       DAOPWB010000250         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_56713_length_2104_cov_4.494388, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000250 DAOPWB010000000
VERSION     DAOPWB010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56713_length_2104_cov_4.494388"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1173)
                     /locus_tag="PKN92_13375"
     CDS             complement(178..1173)
                     /locus_tag="PKN92_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49830.1"
                     /translation="MKQEIHITKWSMCLRLVLFMCVCMITADAFSVNAPVTPEMLLSL
                     RASQGMDISRDPLNVTPIMQEGQIVSVERKWDKATLDSAMFFLTRAFGIDISYEKAVD
                     QEFSAEKRSFEYSVGVNERLVDALEKLVEASEGYIRWRLLDGRIVLTAMPDDDRITAG
                     DRTLQVDIDTATMGEALFQLEKAYNEQHTDVPLAFYTRDYGMFHSRAVKDDRFRVKGQ
                     DTLRNIIISLMNQWDPAKAYYSLNIHRSVHKKYARQYFNDNSLYYYRLEIMDMEACRE
                     EEKRIRQGVPDEGFEEGYHWANAQFPEMSQRLMGYFDRIYPDWAAGEDAASAQEK"
     gene            complement(1192..1962)
                     /locus_tag="PKN92_13380"
     CDS             complement(1192..1962)
                     /locus_tag="PKN92_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49831.1"
                     /translation="MIQLVLIMVLAPFGAPEIVVDQDGLVTLIESPEERANEEAFSGA
                     VRAYNDYRNNGKGDLEELIAQHERILESDRELVPKGRQMIGNQLADLRRERDGASIAI
                     SPERYYEIKEHMNPFFSDNFKDKEEEIAWLQYKEIDLEYEEQRIENLPEEDAIEKARR
                     FYELALTAANTRDAKSRQQCARYQGRSRQKALEAAQKEDMWQREALALVETAGDFLLS
                     ELKSLKNAALCREFIAAYPDDKELVERIEKLLGELESN"
BASE COUNT          481 a          584 c          444 g          595 t
ORIGIN      
        1 ggacatttga atgatgttgt tgctgcatgc tcatgatgat ttcccttact atatatatgc
       61 agtgctgatc atctgattcc atgctagcac aaaggtcgtt agaagtcaag cattttgact
      121 gcccgacctt cacttctttt atccgccatg ggaagggtat ctcaatcata cctgtgccta
      181 tttctcctgc gcggatgctg catcttcccc ggccgcccag tcaggataga tgcgatcaaa
      241 atagcccatg agcctttggc tcatttcggg aaattgtgca ttcgcccagt gatagccctc
      301 ttcaaagcct tcatccggca caccttgcct gatacgtttt tcctcttctc tgcacgcctc
      361 catatccatg atttccaatc tgtaataata aagagaattg tcattaaaat attggcgtgc
      421 atatttcttg tgaacagacc tatgaatatt caagctataa taagctttag ccgggtccca
      481 ttgattcatg agcgatatga taatgttccg cagcgtgtcc tgacctttta cacgaaaacg
      541 gtcgtccttc accgccctgg aatgaaacat gccataatcc cgcgtataaa aagcaagggg
      601 cacgtctgtg tgctgctcat tgtacgcctt ttccagttga aacaatgcct ctcccatggt
      661 tgcagtatca atgtctactt gcagcgttct gtcgcctgct gtaatgcggt catcatcggg
      721 cattgccgtc agcacaatac gaccatccag aagccgccag cgaatatatc cctccgacgc
      781 ttccacaagc ttttcaaggg cgtccacaag ccgttcgttc acccctacgc tgtactcaaa
      841 actgcgtttc tcagcagaaa actcctgatc caccgctttt tcataggaaa tatcgatgcc
      901 aaatgcccgt gtcaaaaaga acatggcaga atccagggtc gctttgtccc atttgcgctc
      961 cacagacaca atctgacctt cttgcataat cggcgttaca ttcaaagggt ctctcgaaat
     1021 gtccatgccc tgactggcgc gtaaggagag caacatctcc ggcgtaaccg gtgcattcac
     1081 ggaaaaggca tctgcagtga tcatgcagac acacataaag agcacgagac gcaaacacat
     1141 gctccacttt gtgatatgaa tttcctgttt cattctgaat atcccttcgt tttaattgga
     1201 ttccagctca cccagcagtt tctctatcct ctccaccagt tctttgtcgt cgggataggc
     1261 cgctatgaat tcgcgacaga gtgctgcatt cttcagactc ttcaactccg agagcagaaa
     1321 gtccccggcg gtttccacca gcgccagagc ctcacgttgc cacatatcct ccttttgagc
     1381 ggcttccagt gccttctgcc gggaccggcc ttgatagcgg gcacattgct gcctgctctt
     1441 tgcatcccgg gtattggccg cggtcagcgc cagctcatag aaccgccggg ccttttcgat
     1501 tgcatcctct tccggcaggt tctctatgcg ttgttcctca tattcaagat caatctcttt
     1561 gtactgcagc cacgcaatct cctcctcttt atctttgaag ttatcggaga aaaaggggtt
     1621 catatgttct ttaatttcgt aataacgttc cgggcttatg gcgatagatg ccccgtcgcg
     1681 ttcacgccgc aaatcggcga gctggttacc aatcatttgc cgccctttcg ggacaagctc
     1741 cctatcggat tccagtatcc tttcgtgttg ggcaatcagc tcctccagat cgccttttcc
     1801 gttattgcga tagtcgttgt aagcacgcac cgccccggaa aaggcttcct cgttcgcacg
     1861 ttcttccggc gattcaataa gcgttaccaa gccatcctga tcaacaacga tttccggtgc
     1921 accaaatggt gccaggacca taatcaatac aagctgtatc atgagtgttc ctcctgtgtc
     1981 tgtgtgttca cccacggctt cgctttgcgg gcgaactgct tttcatcttg tcatggtaaa
     2041 tagagcatta tgataacgta ccatagtttc tgtaaattat ttggacaaac ggggtcatcg
     2101 tttg
//
LOCUS       DAOPWB010000251         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_57775_length_2077_cov_4.020772, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000251 DAOPWB010000000
VERSION     DAOPWB010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57775_length_2077_cov_4.020772"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..1903
                     /locus_tag="PKN92_13385"
     CDS             476..1903
                     /locus_tag="PKN92_13385"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HNZ49832.1"
                     /translation="MESIQQIVVRAVEEIFKTIQGTGLENIGKVVKALVSVTSGMTLE
                     IVESCIEQMDESLVYAAKAQRKQDGITVKERAVERVLTTELGELRYKRTYFAMPNGSY
                     AYILDHLIGVEAYERFSKELIADVLNAATVKSYQHAIASTKQDMSRQTVHNRLVALDD
                     LVTPVEQAEETPETLDIFADEDHVHLTPKGQAMVPLVTMTEGVDKSNPKRHKTIHPIH
                     VAAFGMTAEAFRENVVAVLTERYDLDKVKQINVHGDGAPWIHALQQLLPRSRLVLDGY
                     HLEKELRSFLRLEGAKPYGAAVRQAMRKEDGYEAFERYCESVDRKQSTAKGHERVREF
                     VSYCAQHWDAIVVRMNRETCGSCTEPQVSHVLSDRLSRNPIAWSREGLNRMTMLLVYT
                     KNGGKVRGENVRIRVNSDAKADFSEDGYARYRDYAIKQADEVLKAKHNWSIFERESSK
                     PRKIDWIYMLRKSLGAQQSLVKLIS"
BASE COUNT          581 a          469 c          589 g          438 t
ORIGIN      
        1 gcaatgatct cattggcgta ggtaatggta ccgccgtcgg gcgattctat ctccgcgttg
       61 acgggtaggt tttcattctt aggtgccatg cgacacacct cctcctatat cagagccagt
      121 atagcaaaat tagcgcgccc gcgcaataca gccgggtttc caaccacggg gcgttagtgt
      181 aaagtatttc tgtgagaaat gagttgcagg ggacatagaa agaggaaaga gcgccacgca
      241 ggaccagcga aagcagcgag aagtgtccgg ttagaaagaa tcgtggagca agaaaaagca
      301 gggggtcgtt gcagtagctt tgccggggga actgagcctg cgggagcgta gcgaccaagg
      361 gctcagttcc cccggcggcc gcttatcgaa gggacaaaag gggcagaaaa agaagattcc
      421 atctttgtgt tagactttta gcgcttaaac caaagccaga aagaaggaat cttccatgga
      481 aagtatacag cagattgtgg tcagagcggt agaggaaatt ttcaaaacga ttcagggtac
      541 cggtttggag aatattggaa aggtggtaaa agcccttgtt tcggtaacct ccgggatgac
      601 cctggagatt gttgaatcct gtattgaaca gatggatgaa tcgctggtgt atgcagcaaa
      661 agctcaaagg aaacaggacg gtattaccgt caaggagcgg gcagtagagc gtgttctcac
      721 gacagaactg ggagaactgc gctacaaacg aacctatttt gcaatgccga acggatcata
      781 tgcgtatatt cttgaccatc tcatcggtgt ggaagcatat gagcgattct ccaaggaact
      841 catcgcggac gtgctgaatg cagccacagt caaatcgtac cagcatgcga ttgcttccac
      901 gaagcaggat atgtccagac agacagtgca taaccggctg gtggcactgg atgatcttgt
      961 aacgcctgta gagcaggcgg aagagacgcc ggaaacactg gatatctttg cggatgaaga
     1021 ccatgtccat ctgacgccga aaggtcaggc aatggtgcct ctggtaacca tgaccgaggg
     1081 cgtggataag agcaatccca aacggcacaa aacgatccat ccgattcatg tggcagcctt
     1141 cgggatgacg gcagaagcat tcagggaaaa tgttgttgct gtattgacgg aacgatacga
     1201 tctggacaag gtgaagcaga tcaatgtcca tggagacggg gctccgtgga ttcatgcgtt
     1261 acagcaactg cttccgcgct cacggcttgt tctggatggg tatcaccttg agaaggagtt
     1321 gcggtctttc ctgaggctgg aaggcgcaaa accatatggc gccgcggttc ggcaggcaat
     1381 gcgaaaagaa gacgggtacg aggcttttga aaggtactgc gaaagcgttg acagaaaaca
     1441 gtccacggcg aaggggcatg aaagagtcag agagttcgtg tcatattgcg cccaacactg
     1501 ggatgccatt gttgtccgca tgaacaggga aacctgcggc agctgtactg agccacaggt
     1561 cagtcacgtc ctgtctgatc gtctgagccg caatccgatt gcctggagca gagaaggcct
     1621 gaacaggatg acgatgctgc ttgtctacac gaagaacgga gggaaagttc ggggagagaa
     1681 tgtcagaatc cgtgtgaaca gtgatgccaa agctgatttc agcgaggatg gatatgccag
     1741 atacagggat tacgccatta aacaggctga tgaagtgctg aaagcaaaac acaactggag
     1801 tatcttcgaa cgtgaatctt ccaagccgag aaagattgac tggatatata tgctccgtaa
     1861 gtccttgggc gctcagcagt ccctcgttaa actcatctcc tgatataaac tccgtaacta
     1921 cttgacacaa acccacgggg cggtaactgt tgcaggttcc cctgccctct gctacactac
     1981 aggcaaaacc aatacactcc aaaagaggat atgcagtatg ctgaactttg atttttacgc
     2041 cccggcccgc atcatctttg gcaaggatac ccaacag
//
LOCUS       DAOPWB010000252         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_58679_length_2056_cov_5.357821, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000252 DAOPWB010000000
VERSION     DAOPWB010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58679_length_2056_cov_5.357821"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PKN92_13390"
     CDS             complement(<1..889)
                     /locus_tag="PKN92_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNZ49833.1"
                     /translation="MKRKGFTLIELLVVIAIIGILAAILLPALARAREAARRSSCQNN
                     LKQWGLVFKMYANESPGEKYPRIQVGEFPQLNNDGLPSGTNFVFDLGPNTFSLYPEYL
                     TDPNIIFCPSDPELGQHKKDILDSTGQPNLSGVRPTSKFASAVDSSYTYLGWCFDRFG
                     YEEPGLSCNAILQIMQIVGEANKVPAGAENYTGPTQLIAGVEGLFGKLAPLYTANPPN
                     LDGIAQLTDKDIEVDAAYTGVGNGGGRTIYRLREGIERFLITDINNPAASAQAQSTLP
                     IMFDHVAVSLNLFNHVPGGS"
     gene            1143..1219
                     /locus_tag="PKN92_13395"
     tRNA            1143..1219
                     /locus_tag="PKN92_13395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Arg,seq:ccg)
     gene            complement(1394..>2056)
                     /locus_tag="PKN92_13400"
     CDS             complement(1394..>2056)
                     /locus_tag="PKN92_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNZ49834.1"
                     /translation="EERPLKERLTNRGSKAKLTPATIDKLKWLGRTRSLAYRTLAYTG
                     LRRSELRSITIGAAHLDSEPAYLLLQAENEKNRKGSRIPLHADLILALKEYRQERLKR
                     LAGDCAAFPGTFDAAPFFDGLPKELVRVFDKDLEAAGISKKDGAGHVLDIHALRNTFC
                     TMLARAGVGLATTQKLMRHNDPKLTANIYTILELTDTEKAVNSLPGIDTSREEKKVSS
                     SE"
BASE COUNT          468 a          492 c          552 g          544 t
ORIGIN      
        1 tggatccgcc ggggacgtgg ttgaagaggt tgagtgatac ggctacgtgg tcaaacatga
       61 tgggcagggt gctttgtgcc tgcgcactgg ctgccggatt gttgatgtcg gtgatcagga
      121 agcgttcaat gccttctctg agacggtata tagtgcgtcc tccgccattt cctacgcccg
      181 tgtaggcggc atccacttca atgtctttat ccgtcaattg ggcaatgcca tcaagattag
      241 gcggattggc ggtatatagc ggcgcaagtt ttccgaacaa cccttcaaca ccggcaatga
      301 gttgtgtggg tccggtgtag ttttcggcgc cggcaggcac tttgttcgct tcgccgacaa
      361 tctgcatgat ctgcagaata gcgttgcagc tgagacctgg ttcttcataa ccgaagcggt
      421 caaagcacca gccgaggtaa gtgtaactgg aatctacggc actggcaaat tttgaggtgg
      481 gacggacgcc ggacagattg ggctggcctg tgctgtcaag aatatctttt ttgtgctgac
      541 ccagctcagg atcagagggg cagaaaatga tgttaggatc ggtcagatac tccgggtaga
      601 gggaaaaagt gttcgggccc aagtcaaaca caaagttagt tccgctgggc agaccgtcat
      661 tattgagctg cggaaattct cctacctgaa tgcgggggta tttttcgccc ggcgactcat
      721 tggcgtacat tttaaatacg agaccccatt gcttgagatt gttctggcag gaggatcggc
      781 gcgccgcttc ccgggcacgg gcgagggctg ggagcaggat ggctgcaagg atgccgataa
      841 tggcgatcac gaccagcaat tcaatgagtg taaatccttt tcgtttcata agatgtctcc
      901 tgatggtttg tatgtatccg cagcatgttc ccgggtggaa catgcaaagg ttccatggag
      961 gtagctcttc gcgtattgca cgggagcggt atatccgaaa gcgcaagatt tcagcactat
     1021 gattgttgtc gtaaaaaaag tatagcacat tcctgggtac ttgtcaacgg cagaaatagg
     1081 ggcatgtggg actcaccatt agacacttcc gtcttcggta tggtatgata cagatatatt
     1141 ttgcgcccat agctcagttg gatagagcgt ctgcctccgg agcagaaggt cgcaggttcg
     1201 agtcctgctg ggcgcgccat ttcccacctt cattttaccc ctgtaaacat tggctataat
     1261 gccattttgc cctctcaaaa aacttgtaag ccttccatca acaataatgc caaatatacc
     1321 ccttccgtga cataaaaagc cacacttgtg acaagtagga gtacaagtgt tcacggggga
     1381 tatttcgggg aagttactca ctgctgctca ctttcttttc ttctctgctg gtatcgatac
     1441 cgggaagaga attcaccgcc ttctctgtgt ccgtcaactc cagtatggtg taaatgtttg
     1501 cggtgagttt agggtcattg tgtcgcatca gcttttgtgt cgttgccagc ccaacaccag
     1561 cccgggcaag catagtacag aaagtgttcc ggagagcatg aatatccaac acatgccctg
     1621 ccccatcctt ctttgaaata ccagccgctt ctaaatcttt gtcaaacact ctaaccagtt
     1681 ctttggggag tccgtcaaag aaaggtgccg catcgaaggt gccggggaaa gccgcacagt
     1741 cgccggcaag gcgtttaagt cgctcttgtc ggtattcctt caaggcaagt atcaaatcag
     1801 cgtgaagggg aattctggag cctttgcgat tcttttcgtt ttctgcttgg agcaagaggt
     1861 aagccggttc agagtctaag tgagccgccc ctatggtgat gctccttaat tcgcttcgcc
     1921 gtagtcccgt atatgcaagt gtgcgataag caagggatct tgtccgccct aaccatttca
     1981 gtttgtcaat ggtcgccggg gtcaacttgg ctttacttcc cctattggta agccgttcct
     2041 ttaagggacg ttcctc
//
LOCUS       DAOPWB010000253         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_59352_length_2041_cov_5.871601, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000253 DAOPWB010000000
VERSION     DAOPWB010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59352_length_2041_cov_5.871601"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="PKN92_13405"
     CDS             complement(<1..1547)
                     /locus_tag="PKN92_13405"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HNZ49835.1"
                     /translation="MKSFSNRLLYAATVAGLVLTGAVLQGCSDKIPPGKVEAFRAEAG
                     DGTVALSWTNPADKDLAGVQIRRKAGAMPTASDEGLEIFKDTGTGLADSTVTNGTPYY
                     YAARAYDRAGNYSEPVYANATPVSTLARVEVLDQLAAMTQNISQSPALTRKEQAEMLD
                     ILQEAGTLFISGQPCDAAELLRADFLEKAQELRAGSARKEAEEYYAEGRMIRVNIART
                     MADKDKCPELSRIDAEAETLVRRETPEGLLGEEIFGEPILTTLLPEDSPLPGEVFSQI
                     FIPGTDALHGEAGAPAVPMYRQLVAAPMGRGVRVWVREVDPVPAEEIFLNLYPIQDSP
                     MDQEVDPYDFSDRPFSINRQIYSSNDPWPRQPVSVKYLGNGRDMEFYLVEAAAGQYYP
                     LENRLVLFESSPYEIAFLGGNGSFATENMQSPFDSNSRYLMENVLNKVTVSANIRERI
                     REDIFGEEFLILTHPDFEQAALELRDWKREKGIWANVFTCGTDTDLHDMQTADDIDAF
                     IESRYTHG"
     gene            complement(1730..>2041)
                     /locus_tag="PKN92_13410"
     CDS             complement(1730..>2041)
                     /locus_tag="PKN92_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49836.1"
                     /translation="IRTFGRDVRRSAFETRRLSSLPSRKRQPANCIHHIARGLHRLQA
                     LITKAGSGTRMKTHALFPVSPPERSRQSLIFSGFSGMLRTGQHVNYNPFRSDAYSPDG
                     A"
BASE COUNT          435 a          537 c          549 g          520 t
ORIGIN      
        1 ccgtgcgtgt agcgggactc gataaatgca tcgatgtcat ctgcggtttg catatcgtgc
       61 agatcggtgt cggtgccgca ggtgaagaca tttgcccaga tccccttttc acgcttccag
      121 tcccggagtt cgagtgccgc ctgttcaaag tccggatggg tcagaatcag gaattcctcg
      181 ccaaaaatgt cttcgcgaat tctttcacgg atattggcgg acaccgtcac tttgttcaga
      241 acgttttcca tcagataacg tgaattgctg tcaaaggggc tctgcatatt ttccgtggca
      301 aaagagccgt tgcctccaag aaaagcgatt tcatagggac tgctttcaaa gagcacgagt
      361 ctgttttcca gagggtaata ctgccctgct gccgcttcca caaggtaaaa ctccatgtcg
      421 cggccattgc ccagatactt gacggagacc ggctgccggg gccagggatc gttgctgctg
      481 taaatttgtc tgttgatgct gaagggtcga tccgagaaat cataagggtc aacttcctga
      541 tccatcggag agtcctgtat cggataaagg ttgagaaaaa tttcctcggc aggcacagga
      601 tcaacttctc ttacccagac tctgacgccg cggcccatag gtgccgctac cagctgcctg
      661 tacatcggca ccgccggtgc acccgcttct ccgtgcagcg cgtctgttcc gggtatgaaa
      721 atctgtgaaa agacttcacc gggcagaggc gagtcttccg ggagtagggt ggtaaggatc
      781 ggttcgccga agatttcttc acccagcagc ccttccggag tttccctccg gacaagagtt
      841 tccgcttcgg catcaatacg gctcagctca gggcatttgt ctttgtccgc catggtgcgg
      901 gcaatgttga cccggatcat tcgcccttcc gcataatact cttccgcttc ttttcgtgcg
      961 cttcccgccc ggagttcctg ggctttctcc agaaaatccg cacgcagcag ctccgcagcg
     1021 tcgcagggct gtccgcttat aaaaagcgtt cccgcctctt gaaggatatc cagcatttca
     1081 gcctgctctt tacgggtgag cgccgggctt tgggaaatat tctgggtcat cgctgcaagc
     1141 tgatccagta cctcaacccg cgccagtgtg gagacaggcg tggcattggc atataccggc
     1201 tcggagtagt tgcctgctct gtcgtaggcg cgtgctgcgt agtaataagg ggttccgttg
     1261 gtaacggtgc tgtcggcaag cccggtgccc gtgtccttaa agatttccag tccctcgtca
     1321 ctggcagtgg gcatggcgcc cgcttttcgc ctgatctgta cccctgccag atctttatcg
     1381 gcgggatttg tccaggacag ggcgaccgtc ccgtctcccg cttctgcgcg gaaggcttcc
     1441 acttttcccg gaggaatttt gtcgctgcat ccttgaagca cagcgcctgt caagacaagc
     1501 cccgcgacgg ttgcagcgta aagtaatcga ttactaaacg atttcatcac tttctccttg
     1561 atcttttgtt tgggttctga ctatttgcag tggaaagagg ctttaaataa cgagaacgct
     1621 caaatagatt tttttatttt cgaactaatc atgttttcgt tcaacacaga cagattatac
     1681 ctgtgttttt ctgaaaaagg aaagaaaatt aacaatactg aatgtctttt cacgcaccat
     1741 ccgggctgta tgcgtctgag cggaaagggt tgtaattaac atgttgtccg gtacgcagca
     1801 ttccgctgaa gccggaaaaa atcagcgact ggcgggagcg ctcaggggga gagaccggga
     1861 aaagggcatg tgttttcatc cgggttccgg agcctgcttt tgtgatgagc gcctgcaggc
     1921 gatggagacc tcttgcaatg tgatgaatgc aattcgccgg ctgccttttc cttgaaggaa
     1981 gggaagacag ccggcgtgtt tcaaaagccg agcggcgaac atcgcgccca aatgtgcgta
     2041 t
//
LOCUS       DAOPWB010000254         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_60301_length_2021_cov_3.107833, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000254 DAOPWB010000000
VERSION     DAOPWB010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60301_length_2021_cov_3.107833"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1453
                     /gene="lhgO"
                     /locus_tag="PKN92_13415"
     CDS             227..1453
                     /gene="lhgO"
                     /locus_tag="PKN92_13415"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HNZ49837.1"
                     /translation="MDCKLRDLIIIGGGIVGVAAARMLARECSVSILLLEAEKHLAAH
                     QTGRNSGVIHSGLYYRPGSMKAKTCREGREALYRFCEEKGVPHERCGKVVVAVTEKEK
                     AQLAELARRGEANGLTGIRLLDRAGLQAHEPHAEGVAALHVPETGIVDFSVLTQRFAE
                     EAQVSGAEIRTGVRVTGCEFRDGLFHLDTDIGERFCARTLINTAGLHCDEVAQLCGLR
                     PDIRIIPFRGEYYTLKPERAELVNHLIYPVPDPRFPFLGVHFTRRIGGGIEAGPNAVL
                     AMKREGYSWKDWGAGDLASMLSWPGFWRMGAKYWNTGLQEMYRSFSRRSFARALARLV
                     PEIKETDLQPGGAGVRAQAVDRQGRLLDDFHILEGKQMLHVLNAPSPAATASLAIGRT
                     ITERACALFGFSRRKG"
     gene            complement(1462..>2021)
                     /locus_tag="PKN92_13420"
     CDS             complement(1462..>2021)
                     /locus_tag="PKN92_13420"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNZ49838.1"
                     /translation="LLGEKLKMECIFGPNYYFDGGEYGNAIATRLPVLKSYNTALPNP
                     TNKEPRGCLSVTVEWQGHPVEILCTHLGLTGLERLSQAQAIAALPGDTPHILAGDMNE
                     RITAPGMKLLSEQLQAAVLIEEEPVVNSCPTPIARTRIDLIFASSGLDILSSKVIQDE
                     RTKVASDHFPCIASFKLKLDSEEKN"
BASE COUNT          422 a          505 c          616 g          478 t
ORIGIN      
        1 ataccatact ttactgtttt gtcaagtggt atattatgtg gtattatctt ccgggaagta
       61 acgctcagcg tgcttgttgg acgacattgg ctggagcgta atttctgaat aaagctctca
      121 ttccggatca gaatttgctc ctgccatgca atctctggaa cagtatgtcc caaggtgtct
      181 gccgaaaaag atgctggtgt atttttaaac atgggacaac aaagcaatgg actgtaagct
      241 ccgggatctg attattatcg gcggcggtat tgtgggggtt gctgccgcgc gcatgcttgc
      301 ccgggaatgt tcggtgtcca ttctgctgct ggaagcggaa aaacatctgg ccgcacatca
      361 gaccggcaga aacagcggtg taatccattc agggctttac tacagacccg gctccatgaa
      421 agcaaagacc tgcagagagg gcagggaagc tttgtaccgg ttttgtgaag agaagggtgt
      481 ccctcatgaa cgctgcggca aagtggtggt ggctgtaacg gagaaggaaa aggcgcagct
      541 tgcggagctt gccaggcggg gcgaagccaa cggattgacc gggatccggc ttctggatcg
      601 tgccggactg caggcgcatg agccgcatgc ggaaggtgtg gcggcacttc atgtgcccga
      661 aacgggcatt gttgattttt cagtgcttac gcaacgcttt gcagaagagg cgcaggtctc
      721 aggtgctgaa atccggacag gtgtccgtgt gaccggctgt gagttccggg acggactttt
      781 ccatctggac acggatatcg gggaaaggtt ctgcgcccgg acactgatca ataccgccgg
      841 gctccattgt gatgaagtgg cgcagctgtg cggactgcgt cctgatatca ggattattcc
      901 ctttcgtggt gaatattaca ccttgaagcc ggagcgtgcg gaactggtta accatctgat
      961 ctatccggtg cccgatccgc gctttccttt tctcggcgtg cattttacac gtcggatcgg
     1021 cggcgggatc gaagcagggc ccaatgcggt gctcgccatg aagcgtgagg gatactcctg
     1081 gaaagactgg ggggcaggtg atcttgcttc catgctgtcc tggccgggat tctggcgcat
     1141 gggtgcgaaa tactggaaca cgggtttgca ggaaatgtac cggtctttca gccgccgctc
     1201 ctttgcccgg gcgcttgccc ggctggttcc tgagattaag gaaacagatc ttcagcccgg
     1261 cggtgcagga gtccgtgcac aggcggtgga tcgacagggc aggctgctgg acgattttca
     1321 tattctggag gggaagcaga tgctccacgt tctgaatgcc ccgtcaccgg cagccacggc
     1381 atccctcgcc atcggcagaa ccattacaga gcgggcatgt gccctcttcg gcttttcccg
     1441 cagaaaagga taaggggcaa gtcagttctt ttcctctgaa tccagcttca gtttaaaaga
     1501 ggcaatacag ggaaaatggt cggaggcgac tttggtgcgc tcatcctgaa tgactttgct
     1561 ggatagaata tccaggccgc tgctggcaaa gatcaggtcg atacgcgtcc gtgcaatcgg
     1621 tgtcgggcac gaattcacaa cgggctcttc ttcgatgagc acggctgcct gcagctgctc
     1681 ggacagcagt ttcatcccgg gcgcggtaat gcgttcgttc atgtctccgg cgagaatatg
     1741 cggggtgtct ccgggaagtg ctgctatagc ctgcgcttgc gacaggcgtt caagaccggt
     1801 taagcccaga tgggtgcaga gaatttctac cggatgcccc tgccattcca ccgtgacaga
     1861 aaggcagccc cggggctctt tattggtcgg attgggcagt gccgtgttgt agcttttcag
     1921 cacaggcagc cgtgtcgcaa tggcgttgcc gtattctccg ccgtcaaagt aataattcgg
     1981 tccgaagatg cattccatct tcagtttttc gcccagcagc g
//
LOCUS       DAOPWB010000255         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_60478_length_2017_cov_6.473496, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000255 DAOPWB010000000
VERSION     DAOPWB010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60478_length_2017_cov_6.473496"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            730..>2017
                     /locus_tag="PKN92_13425"
     CDS             730..>2017
                     /locus_tag="PKN92_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNZ49839.1"
                     /translation="MFINQTLPIVKRAEFQEEGKMTDDTCRDQESKGLRKHRFCRLFV
                     FTFFVLLFFGSIMVAEGQGSQKASPALLAKVQKAGSVRVIIRLDTSFQPEGQLSGPTA
                     VAGQKARIASVQTRLQQEVSEHNVRGIKRFKHIPYSAMAVDPDALDALIAHPLVLSIE
                     EDKLIPATLTESVPLIKADQAWGLGYTGSGWTVAVLDSGVDKTHSFFPAGKVVAEACF
                     STTDAGQHAETVCPDGNESQTGAGAGIQCGGANGTEIDGCKHGTHVAGIAAGKSASIN
                     GVAKEANIIAIQVFSKITDAASCSSYGLTSPCALTFTSDQNLALEHVYSLRNTYKIAA
                     VNMSIGGGQYFSACDTSESSTKAVIDNLRSAGIATVISSGNEGYKNSVGSPACVSSAV
                     SVGATTKADAEADYSNYHPVMLSLFAPGSAINSSVPG"
BASE COUNT          517 a          464 c          534 g          502 t
ORIGIN      
        1 ggcgggccca tgcatacagt ggcgactacc tggcggaaga tccgcgctgt ttcatccgtg
       61 aaaaacaaaa agctccgtaa tcattcaaac tcgaaagaaa tcttttgact ttgtcctgga
      121 attttatcat tatccttttc agataaaaaa gctgatatcg ggcacagaaa atgaattgtt
      181 cttttgacgg tccgttgctg ttccgggtga cctgaacggt gaacttgcta tcggaaccca
      241 ttctatgggg gactcatgac ccctaaaata atcatcaggg tattttcagg gtttttattt
      301 cttctggtta cattgtttgc aagctgtggc caagccatga acctgtctca aaccgaggga
      361 aaacctcccg tgtcaagaaa tgaacagtca tcttcactcc aacattacag aaatcttctt
      421 acccgtgcgg aagcggttgg cagcgtccgc atcatagcaa gactgaatat gccgtttgtg
      481 cctgacagtc aactgccacc gcaggcggct gttgaccagc agaaccgcat agcagaaata
      541 caggatcgat tatgtgcgga cctgtcagaa tatcaggtca ggagtgttaa aagaatgaag
      601 tacgcacctt tcataggaat ggaggtggat gcaaaggcgc tgagagtgct gatttcccat
      661 ccgctcatcc tgtcattaca ggaggacgct cctgtgccgc cggcaggaag gtaaacggga
      721 ggataggaat tgtttatcaa ccagacactg ccgattgtga aacgggccga atttcaagag
      781 gaggggaaga tgacagacga tacatgtagg gatcaagaat ccaaagggtt acggaaacat
      841 cggttttgcc ggttgtttgt attcacattt tttgttctct tgttttttgg cagtatcatg
      901 gtggctgaag ggcagggcag ccagaaagca tcccctgcgc ttcttgccaa ggtacaaaag
      961 gcgggcagcg tgcgggtcat cataagattg gacacttcct ttcagccgga ggggcagttg
     1021 tcaggaccga cagcagttgc tggccagaaa gcccggatcg caagtgtgca gacccggctg
     1081 cagcaagaag tttccgaaca caatgtaagg ggcattaaaa gattcaagca tatcccttac
     1141 agtgcgatgg cagtggatcc ggacgcgctg gatgctttga ttgcccatcc tcttgtgctt
     1201 tcgattgaag aagataagct tattccggcg acattaacgg agagtgtccc tttaatcaag
     1261 gcagatcaag cctggggact gggttataca ggatcaggat ggacggtggc tgttcttgat
     1321 tctggagttg ataaaactca cagcttcttt cctgccggta aagtggttgc agaggcttgc
     1381 ttttccacca ctgacgccgg ccagcacgca gaaacggttt gtccggatgg caatgagtct
     1441 cagacaggag caggcgcggg aatccagtgc gggggagcaa acggtacaga aattgatgga
     1501 tgtaagcatg gaacccatgt ggctggtatt gccgcgggca agtcggcttc cataaatggt
     1561 gtggccaaag aagcgaacat cattgccatt caggtatttt caaaaatcac tgacgcagca
     1621 tcctgcagca gttacggtct tacttcacct tgtgcgctta cctttacgtc tgaccagaat
     1681 cttgccctgg agcatgttta ttcattgaga aatacctata aaattgcagc ggtcaacatg
     1741 agtattggag gcggtcaata tttttccgcc tgcgatactt ccgaatcttc tacaaaggcc
     1801 gttattgaca acctccgttc ggcgggaatt gccaccgtca tatcatcagg gaacgaaggt
     1861 tataaaaaca gcgtgggttc acctgcctgt gtttcttctg cggtaagtgt gggggcaacg
     1921 acaaaagcgg atgccgaggc ggactatagc aactatcatc ccgtcatgct gtctctcttt
     1981 gcgcccggaa gcgccatcaa ttcttcggtt ccgggcg
//
LOCUS       DAOPWB010000256         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_61032_length_2005_cov_4.688205, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000256 DAOPWB010000000
VERSION     DAOPWB010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61032_length_2005_cov_4.688205"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="PKN92_13430"
     CDS             <1..1366
                     /locus_tag="PKN92_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49840.1"
                     /translation="ASSHCTLVNCLSGYNQTEWDLADQSRFIRSDASWLQTVTADSFT
                     PGTADWSRYCLGAALRLQHQFPAKGLTGVTAMVASDLPVGASLSSSTALNLVSLMAFA
                     AFNGLSLSLPHLLLASRDVEWYAGARSGLSDQAAILAGRRDQIFHVAIDPDDPDVRAG
                     KYIPFPDDLSLLVVNSCTTRMLSGAERVQYSLNRFAYSMALNVLQAELFRAGYPAEKV
                     SRMHRLSEVTPEALGGMSALMNLLQSIPETISLSDLKKRYAPDNLERDYALYFDGVPE
                     KEKPDQIPLRGPLLFGIAESARAQRFPLVLQEKNYALAGRLMSLGHAGDRVASKEGNP
                     FKANVRDTALKNQAAHALPVELIPGWYGASSPALDMLVDAAIDGGAFGASLTGAGIAG
                     AVLALCSRSKEEQVRQAILQCMQSRRYANVSGGDDVLRSTPVQEWVVANHAVSGAGEI
                     LLPF"
     gene            1442..>2005
                     /locus_tag="PKN92_13435"
     CDS             1442..>2005
                     /locus_tag="PKN92_13435"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNZ49841.1"
                     /translation="MLTDTVLHLIGNTPLLQLGSEAIFAKAEFLNPGGSIKDRVALAM
                     LEGAEARGELRAGALIVEPTSGNTGIGIALVGRLKGYDVHIVMPENMSEERKRLIRAL
                     GAQLTLTPAEDSIAGSVKRVQEMKASDPRVFVPQQFENPDNPRAHYENTARELWEQTG
                     GDIACFIAGVGSGGTLQGVGRFLKEHNP"
BASE COUNT          404 a          531 c          607 g          463 t
ORIGIN      
        1 cgcttcgtcg cactgtactc tggtcaactg cctttccggt tataatcaga ccgaatggga
       61 tctggcagat cagagccgtt ttatacgctc ggatgcttcc tggcttcaga ctgttaccgc
      121 agattccttc acgccgggta cagcggattg gagcagatac tgcctcggcg ccgcattgcg
      181 gctgcagcat cagtttcccg ccaaaggtct gaccggtgtg acagcgatgg tggcaagcga
      241 tcttcctgta ggcgcttcgc tcagttcttc cacggcgctg aatctggtct cactgatggc
      301 gtttgctgct ttcaatggcc tttccctttc cctgccgcat ctgcttttgg caagccgcga
      361 tgtggaatgg tatgcgggag cacgcagcgg gctgagcgat caggcggcaa tcctcgcggg
      421 gcggcgtgat cagatttttc atgtggcgat agatcctgat gatccggatg tgcgcgccgg
      481 taaatatatt ccctttcctg atgacctgtc attgctggtg gtcaattcct gtactacacg
      541 gatgctcagc ggagcggagc gtgtgcagta ttccctgaac cgttttgcct attccatggc
      601 gctcaatgtg ctgcaggcgg aactgttcag ggccggatac cctgcagaaa aagtttctcg
      661 tatgcaccgt ctgtcggagg tgacgccgga ggcattgggc ggcatgtcgg cgcttatgaa
      721 tctgcttcag tcaatccccg agaccatttc gctgtccgat ctgaaaaaac ggtatgcgcc
      781 tgataatctg gaacgggatt atgccctcta tttcgacggg gtgcccgaga aagaaaagcc
      841 tgaccagatt ccactgcggg ggccgcttct tttcggtatc gccgaatcgg cacgggctca
      901 gcgatttccc cttgtgctgc aggaaaaaaa ttacgcgctg gcgggacggc ttatgtcgtt
      961 ggggcatgca ggggatcggg tagccagtaa ggagggaaat cctttcaagg cgaatgtacg
     1021 ggacacggct ctgaagaatc aggcggcgca tgccttgcct gtggagctga ttcccggttg
     1081 gtacggcgcc agcagtccgg cgctggatat gctggtcgac gccgccattg acgggggcgc
     1141 attcggcgcc tcccttaccg gtgcaggcat cgccggtgcc gttcttgcct tgtgcagccg
     1201 cagcaaagaa gagcaggtgc ggcaggcgat tctgcagtgc atgcagtccc ggcgttatgc
     1261 gaatgtttcc ggcggcgacg acgtcttgcg ttccacgccg gttcaggaat gggttgtggc
     1321 aaatcatgcc gtctcaggtg ccggagaaat tcttctgccc ttctgaagca gggttgaatt
     1381 gactgattct ttctctgtat cgtagcgctt tagaaaccat tgcaggagaa tcaccgtaag
     1441 catgcttacc gacactgttt tacatctcat cggcaacaca ccgctgctgc agctgggtag
     1501 cgaagcgatt tttgccaaag cggagtttct gaatcccggc ggaagtatca aagacagagt
     1561 ggcgcttgcc atgctggaag gcgccgaagc acggggagaa ctgcgggcgg gtgcgctcat
     1621 cgttgaaccc acttccggaa acaccgggat cggtattgct ctggtcggaa gactgaaagg
     1681 ctatgatgtt catattgtca tgccggaaaa catgagcgaa gagcgcaagc ggctgatccg
     1741 tgccctcggt gcgcagctga ccctgacccc ggcggaagac agcatcgccg gatccgtgaa
     1801 acgggttcag gagatgaaag catctgatcc gagagtgttt gtaccccagc agtttgaaaa
     1861 ccccgataat ccccgggcgc actatgaaaa taccgcccgg gaactctggg aacagacagg
     1921 cggtgacatt gcctgtttta ttgcgggcgt gggcagcggc ggcaccctgc agggagtggg
     1981 ccgttttctt aaagaacata atccg
//
LOCUS       DAOPWB010000257         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_61695_length_1990_cov_4.892506, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000257 DAOPWB010000000
VERSION     DAOPWB010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61695_length_1990_cov_4.892506"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1472
                     /locus_tag="PKN92_13440"
     CDS             180..1472
                     /locus_tag="PKN92_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49842.1"
                     /translation="MSALRFFFPLFMVVGLIMTQVSATEFKAGFAKKDITPQAPLPMW
                     GYGARHDIKASATRDPLFAKVLVIEAGGERAALIGLDLGRSPTAAMMKIIEEGAKEKA
                     GVSYILAVGSHTHHGPVIELLDEPGMGQGKFDDAVKYAEQLPAVLLEAIEEAAASLQD
                     ARIGLAVEDTDLNRNRHTKKLPKPIDPRLSVLRIDDLSGNCLAVMINLAAHATIESVF
                     NLHWTSEWPGHMQKQVEDELGCACFFMQGAAGDMSPNTSDTRKGIDGFGKAAAEKAIE
                     LTKTIQTTIPETPSIGGRTGYFTGPSRIDLSNRLVLGALKQGFFPELLALTVEMPDNQ
                     VTVRMDTLVLNKQFAFVGASGELFSDLSNRIKARSPLRDTLVFGYCNGHSMYIPTREG
                     IEEGGYGADPLVAWCPEGTGEAIVEKAVENIEALVAAQ"
     gene            1674..>1990
                     /locus_tag="PKN92_13445"
     CDS             1674..>1990
                     /locus_tag="PKN92_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49843.1"
                     /translation="MSLDIAASAGTSTILPLHEIQTAVVNRLESRDEMGVTDAVDTIL
                     SQAVYHGSSDIHLEPWTECVSLRYRLDGILQQIAIIPQEFQPKLIARIKVLADLVVYR
                     KDVP"
BASE COUNT          508 a          483 c          487 g          512 t
ORIGIN      
        1 aacccccgaa tcctgaaaat tcatagggtg agattttatg aaacagaact aatttgatga
       61 aagtcttata aaaaaagtat tgtaaaggtg ttctattttt ttaagtgctt gccaaaacag
      121 aaaacaggag gcgcgttgga aaaaagaatg atgccatcgc accctccaaa ggagccgtta
      181 tgtctgcgtt gcgttttttc tttcctcttt tcatggtcgt gggtctgatc atgacacagg
      241 tctccgcaac tgagtttaaa gcaggttttg caaaaaaaga catcacccct caagctccgt
      301 tgccgatgtg gggatatggt gcgaggcatg atataaaggc ctcggcaaca cgcgatccgc
      361 tctttgcaaa agtactagtc atcgaagcgg gtggtgagcg cgccgctttg atcggtcttg
      421 atctgggacg cagccccaca gccgccatga tgaaaatcat tgaagagggg gcgaaagaaa
      481 aagccggcgt ctcttatatt ttggctgtcg gttctcatac acatcacggc cctgtcattg
      541 aactcctcga cgagccgggt atgggtcagg gaaaattcga tgacgctgta aaatatgcgg
      601 agcagctgcc ggcagttctg cttgaagcca ttgaagaagc ggcggcatct ttgcaggatg
      661 cacgtatcgg tcttgctgtg gaagataccg acctgaacag aaaccggcac acaaagaaac
      721 tgcctaagcc tatcgacccg cgcctgtctg tcttacgcat agacgattta tcgggaaact
      781 gtctagctgt aatgatcaat ctggctgccc atgctaccat tgaaagtgtt tttaatctcc
      841 actggacttc cgaatggccc ggacatatgc agaaacaggt ggaagatgaa cttggctgtg
      901 cctgcttttt catgcagggc gcagcaggcg acatgtcgcc caatacttcc gatacacgaa
      961 aaggaatcga cggtttcggc aaggctgcag cagaaaaagc cattgaactg acaaagacca
     1021 ttcagacgac tatccctgaa acgccttcca tcggaggaag aactggctat ttcaccggtc
     1081 cttcccgtat tgatctgagc aatcggctag ttcttggagc gttaaagcag ggatttttcc
     1141 ccgaattact ggcgctcact gtcgaaatgc ccgataatca ggttactgtg cgaatggaca
     1201 cccttgtatt aaacaaacaa tttgcatttg tgggtgcgtc gggcgagctt ttctctgatc
     1261 tatccaatcg tataaaagca cgctcacccc ttcgggatac cttggtcttc ggctactgta
     1321 acgggcacag tatgtacatt ccgacccggg aaggcataga agaaggtggt tacggcgctg
     1381 accccctggt agcctggtgt ccggaaggca caggtgaagc cattgttgaa aaagccgtcg
     1441 aaaatattga agccctggtt gcagcgcaat aacgccgctc tctttataaa aatgggaaaa
     1501 ccaacttctt ttttgttttt gcaggctcgt attctataat cgaatgaatc cggctgagct
     1561 ttagctccct cagaattcat gcgagctgcc tgctgccgga tttttcaggc actcttttct
     1621 gtttacggtc gcactacaat tttgcctttt tccatactgt gaaaggacac aggatgtccc
     1681 ttgatattgc agcctctgca ggcacttcca ccattcttcc tttgcatgaa atccaaaccg
     1741 ctgtggttaa ccggttggaa agccgtgatg aaatgggtgt caccgatgct gttgacacta
     1801 tcctcagcca agccgtatat catgggagca gcgatatcca tctggagccc tggactgaat
     1861 gcgtatcgct gcgctatcgc cttgacggaa ttttgcagca aatcgctatt attccgcagg
     1921 aatttcagcc caaactgatt gcccggatta aagtgctcgc tgacctggtc gtctatcgca
     1981 aagacgttcc
//
LOCUS       DAOPWB010000258         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_63351_length_1956_cov_5.938453, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000258 DAOPWB010000000
VERSION     DAOPWB010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63351_length_1956_cov_5.938453"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..85
                     /locus_tag="PKN92_13450"
     tRNA            9..85
                     /locus_tag="PKN92_13450"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Met,seq:cat)
     gene            complement(127..>1956)
                     /locus_tag="PKN92_13455"
     CDS             complement(127..>1956)
                     /locus_tag="PKN92_13455"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNZ49844.1"
                     /translation="TALEYAHTKGVLHLDIKPANMIIHKNKEGQINVKLTDFGIGQTR
                     RDISLTSAVTALTFTSEGGVGTPAYMAPEQIEPDRFGKFGPATDLYSFAVMLFEMLTL
                     RLPFSGAYTEVLHAHANTSPPDPRDFNPSIPASLARVVLKGLRKSPSNRHANAEEMLL
                     DFQGRLDPNQRVKNTFKLPKDKKLSTLSTVKKLFSQQVRLVVLVLLLTGVAFFTWRSF
                     FSGEKGPGSGGMSREEARASAAKFRDDLEKTWGDDLKNKEYRDAMELFEKAEKAESGR
                     ESVQLFFEAQSTFVALKKQKEADRKQQSEDEKKAAEQAEIAAEKKKAKEEEEKIADAP
                     KASPLVRVQNEVIDLGNGISLEMIWVPSGVFLMGDDVYRGPAKKGGSKSSDTSPAREI
                     WIPKAFWIGKYEVTQAQWKAIMGNNPSRFNNSEQNPVDSVTWNDCQLFIQKLNARVPG
                     GGFRLPSEAEWEYATRAGSNAPFFYGSSSDKLKDYGWFSFNSAGSSQEVGKKTGNTWG
                     LCDTNGNVMEWVQDWYDADYYKVAPSAYPVGPATGTHKVLRGGSWSMTAENCTSAVRH
                     YAMPDMALDTYGLRLVRNASDEKKERAASEEKQEQAASEEKQE"
BASE COUNT          392 a          519 c          506 g          539 t
ORIGIN      
        1 ccagatgagg cggtgtagct cagttggtta gagcagtgga atcataatcc acgtgtccgc
       61 ggttcgaatc cgtgcaccgc taccagcaaa ttcaacggtg atccgaacta tcgggtcacc
      121 gtttttttat tcctgttttt cttcagaggc agcctgttcc tgtttttctt ccgatgcggc
      181 acgctccttc ttttcgtcgg aggcattccg cacaagccgc agtccgtagg tatccagtgc
      241 catatccggc attgcataat ggcggacagc actggtacag ttttctgctg tcatgctcca
      301 ggaaccgcca cgcagcactt tgtgggtgcc cgtagcaggg ccgacaggat aggcggatgg
      361 tgccactttg taataatccg catcgtacca gtcctgtacc cattccatca cattgccgtt
      421 ggtatcgcaa agtccccagg tattgcctgt tttctttccc acttcctgcg acgatccggc
      481 actgttgaaa gaaaaccagc cgtagtcttt taacttgtcc gaactggagc catagaagaa
      541 gggggcattg gaaccggcgc gggtggcata ttcccattcc gcctctgaag gcagtcggaa
      601 accgccgcct ggaacgcggg cattgagttt ctgaataaac agctggcagt cattccatgt
      661 gaccgagtct accggattct gctcgctgtt gttaaaacgg ctggggttat tgcccatgat
      721 ggctttccat tgtgcctgtg taacttcgta tttgccgatc cagaaggcct tcggaatcca
      781 aatttcccgg gcagggctgg tgtcactgct tttgctgcct ccttttttgg cggggccgcg
      841 atatacatca tcgcccataa ggaacacgcc ggatggcacc cagatcattt caagagaaat
      901 accgttaccc agatcgatca cctcattctg cacacgaacc agggggctgg cttttggcgc
      961 atccgcgatt ttttcttctt cttcttttgc ttttttcttc tccgctgcaa tttctgcctg
     1021 ctcagcggct ttcttttcgt cttccgattg ctgtttacgg tctgcttctt tttgtttttt
     1081 cagtgcgaca aaggtgcttt gtgcctcaaa aaagagctgt accgattccc gcccgctttc
     1141 cgctttctcc gccttttcaa aaagttccat ggcatcgcgg tactctttgt ttttcaagtc
     1201 atcgccccag gttttttcca ggtcgtccct gaattttgcc gcacttgccc gggcctcttc
     1261 gcggctcatg cctcccgagc ccgggccttt ctctcctgaa aagaaggatc tccaggtgaa
     1321 aaaggcgacg cctgtcagaa ggagcacaag aacaaccagc cgaacctgtt gggagaataa
     1381 ttttttaaca gtcgataagg tgcttaattt cttatcttta ggcagtttga atgtattttt
     1441 aacccgctga ttcggatcca gacggccctg aaagtccagc agcatttctt cggcattggc
     1501 atgacggttg cttggagatt tgcgaagccc tttcagtaca acccgtgcca gtgaggcagg
     1561 gatggaaggg ttgaaatcgc gcggatcggg cggcgaagtg ttcgcatgag catgcagtac
     1621 ttcggtgtag gcgccactga aggggagccg cagtgtcagc atttcaaaga gcatgactgc
     1681 aaagctgtac aggtctgtgg caggaccgaa ttttccgaag cgatccggct cgatctgctc
     1741 aggcgccata taggcgggcg tgccgacacc tccttcgctg gtgaaggtca gcgccgtaac
     1801 ggctgaggtc agactgatat cacggcgggt ctggccgatg ccgaagtcgg tcagtttgac
     1861 gttgatctgc ccctctttgt tcttatggat aatcatattg gcaggtttga tatccagatg
     1921 aagtacgcct ttggtatgag cgtactccag agcggt
//
LOCUS       DAOPWB010000259         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_63887_length_1945_cov_4.737566, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000259 DAOPWB010000000
VERSION     DAOPWB010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63887_length_1945_cov_4.737566"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..981)
                     /locus_tag="PKN92_13460"
     CDS             complement(79..981)
                     /locus_tag="PKN92_13460"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNZ49845.1"
                     /translation="MPVTHEKRIAVLAPVLLSLFCMCLTAVAQPAERTVVANRSERTL
                     HQFQGKDGAVTFTNRPEKYKHRRDFVEVVIKYERIPLPPQYKPTPVAQPKVVTDENLR
                     AIVSRYASLYGLSEPLVYAVIRAESNFNPNAVSPAGARGLMQLMPGTAAEMGVTDIFD
                     PTQNVAGGTQYLSKMFSLFNDKRLALAAYNAGPENVKKHKGIPPFEETKNYVRMVMAF
                     EEAYAKGAKMPVMAVSGSSGAGMQIAQPQAKPSDKKAFIVHFHSGLTQPADQVHDKDP
                     YWYIEYGPRVYPVRKDLVKAIEKA"
     gene            1127..1573
                     /locus_tag="PKN92_13465"
     CDS             1127..1573
                     /locus_tag="PKN92_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49846.1"
                     /translation="MKKEFVSILKCADAAEAAFYTDYLAARGIPVTNTIETMRAWTGR
                     YAQLSGKPVLRVQKEYVAEARKLLKEVPEADYSEMEQLAEKQAETWLETDTLDHCPLC
                     GSSQIDSIPAGSLAGWLVWLFSLGLFKPDGTPFWVCRDCDWDSRRK"
     gene            complement(1639..1764)
                     /gene="ykgO"
                     /locus_tag="PKN92_13470"
     CDS             complement(1639..1764)
                     /gene="ykgO"
                     /locus_tag="PKN92_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNZ49847.1"
                     /translation="MRVLSSLKSAKKRHPACKIVRRKGRLYVINKKNPRFKARQG"
BASE COUNT          496 a          481 c          484 g          484 t
ORIGIN      
        1 acagccgcag cccggagcat tttctggatc tttttgtgga ggtggtgcag gcaggggacg
       61 aggaaggcta aaaacgtatt atgccttttc gatcgctttg accagatcct tgcggacggg
      121 ataaacacgg ggcccgtatt caatatacca gtagggatct ttatcgtgca cctgatcggc
      181 gggctgagtg agtccgctgt gaaaatgaac aatgaaagct ttcttgtctg aaggcttcgc
      241 ctggggctgt gcgatttgca tgcctgctcc cgaagatcca gaaactgcca tcaccggcat
      301 tttcgcccct tttgcatagg cctcttcgaa tgccataacc atgcgcacgt agttttttgt
      361 ttcctcaaaa ggcgggatcc ctttatgttt ttttacattt tccggtcccg cattataagc
      421 cgccaaagca agccgcttgt cattaaaaag actgaacatc tttgacaggt actgcgtacc
      481 gcctgccaca ttctgcgtag gatcaaaaat atccgtaacc cccatttctg cagcagtacc
      541 cggcatcaat tgcatcagcc ctcgggcacc tgccggtgac accgcattcg gattgaaatt
      601 gctttctgcg cggatgaccg catagacgag aggctcgctc aggccgtaca gggaagcgta
      661 acggcttaca atggcgcgta aattctcgtc tgtcaccact ttaggctgcg ccacaggggt
      721 aggcttgtac tgaggcggca aaggaatccg ttcgtactta attaccactt caacaaaatc
      781 acggcggtgc ttatattttt ccgggcggtt ggtaaaagtg acagccccgt ctttgccctg
      841 gaactgatgt aaggttcgtt cgctgcggtt ggcaaccaca gtgcgttcgg caggctgtgc
      901 caccgctgtg agacacatgc aaaaaagaga aaggagcacc ggcgcaagca cagcaatccg
      961 tttctcatga gtgacaggca tcagcagttt cctatgaatt cccataatag tcaagagtat
     1021 accatgaccg ccgaaaagac gcaagcgcaa aaaaaggcgg catgcttttt tgatcagggc
     1081 actccctgcg gttgtataat tttttttaac gaataaaggc tttcccatga aaaaagaatt
     1141 tgtaagcatt cttaaatgcg ccgacgcagc agaagccgct ttttatacag actatcttgc
     1201 cgcccgcggc atcccggtga ccaacacgat cgaaaccatg agggcgtgga cagggcgtta
     1261 tgcacagcta agcggaaagc ccgtgcttcg cgtacaaaaa gaatacgtag ctgaagcacg
     1321 caaactgctt aaggaagtgc cggaagcgga ctattcagaa atggaacaac tggcggaaaa
     1381 gcaggcggaa acatggctgg aaactgatac tctcgatcac tgcccgctgt gcggctcttc
     1441 ccaaattgat tccattcctg cgggcagtct cgcagggtgg ctcgtttggc tttttagcct
     1501 cggactgttt aagcccgatg gcactccttt ctgggtctgc cgtgactgcg actgggattc
     1561 acgccgaaaa taaacaaagg ctgctgcctg atcggaggat ccagacagca gcctgacata
     1621 ttacgtgatg tgataaaatc agccctgacg cgctttgaag cgggggttct ttttgttaat
     1681 tacatagagg cgcccttttc tgcggacaat tttgcatgca ggatggcgtt tcttggcgct
     1741 tttcagagag gataacacac gcattactga tactccttaa cattactatt tgaaaaagtg
     1801 atagacagaa taattacacc tcttccctca acctctatag ggtagcgtat ttaactacgc
     1861 tatgtcaaat ttttgtctcc ggtattcatc gcgcgggttc aaattcgaaa cgcaattatt
     1921 gagaaacaaa aaaggagaca ttgcg
//
LOCUS       DAOPWB010000260         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_65472_length_1914_cov_4.362023, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000260 DAOPWB010000000
VERSION     DAOPWB010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65472_length_1914_cov_4.362023"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PKN92_13475"
     CDS             <1..435
                     /locus_tag="PKN92_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HNZ49848.1"
                     /translation="REQGAKRLALLLGIAPFIVLFCAWIGMTSHVYLARLHPDIRLTE
                     RVLAEERGEVEGWTIESEAFRSGKVPIPALLESAHIIQKRYKRGAAWLGAFMGVVIAG
                     MIARLSIVRRRADYEANRTYCVSCARCFAYCPVEEESPCVTQ"
     gene            422..>1914
                     /locus_tag="PKN92_13480"
     CDS             422..>1914
                     /locus_tag="PKN92_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNZ49849.1"
                     /translation="MLRNKSRNFIQKPYLFQLSALVAFASGLFILIICVLMIANYVQI
                     VAMDPVDQPELLALREEFARTPDLDPVMVDRIRALDFLSRKAYFTSQTQLNTGARLLF
                     GAAVVFLISMRLAARWNPKPPGPPLQSATKGHWGRQALIRELIILSAMVLMIAAALAA
                     LFTPLEFPPLEQHAQTSAVEAAGDTDAPETAAVTFPVWEDLQKQWPSFRGPGGYGLAH
                     AESVPLKWDGESGKNIRWKTALDAPGFNSPVVWGDSLFFSSADETKRTVHCYDTDSGE
                     LKWMQVLPSFPGTPETPPKVSADTGYAAPTMVAHYPYVYAIFANGDLACYDFSGKLIW
                     GKNLGVPENHYGHASSLIAWENLLYIQYDQKKNPRLLALKADTGAPAWEVQRKKISWA
                     SPIIIQRTSGPQLILASEKDVDAYSLSDGTLLWTVQCLDGEVAPSPAYTRDRVFVGQD
                     YAAATAILLHDEESSTTAEATWEWDDSLTDIASPLGIGDYFIMATSRG"
BASE COUNT          398 a          495 c          540 g          481 t
ORIGIN      
        1 cgggagcagg gggcgaagcg tcttgcactg ctgctgggta ttgccccttt cattgtgctg
       61 ttctgtgcct ggattggcat gaccagtcat gtttatcttg cccggctgca tccggatatc
      121 cgcctgacgg agcgggtgct ggcagaagaa cggggcgagg ttgagggctg gacaatagaa
      181 agcgaagcgt tcagaagcgg caaagttccc atcccggctt tacttgaatc tgcgcatatc
      241 attcagaaac gctataaaag aggtgccgcc tggctgggtg cctttatggg tgttgtgatt
      301 gcgggtatga ttgcccgtct ttccatcgtg cgccgccgtg cggattatga ggcgaacaga
      361 acctattgtg tgagctgcgc tcgctgtttt gcctattgtc cggtagaaga ggaatcgccc
      421 tgtgttacgc aataaatcgc ggaatttcat acaaaaaccc tatctttttc aattgagcgc
      481 attggttgct tttgcatccg gtctctttat tctgatcatc tgtgtcctca tgatcgccaa
      541 ttatgtacag atagtcgcca tggatccggt ggatcagcct gagctgcttg cattgcgtga
      601 ggaatttgcc cggacgcccg atctggatcc ggtgatggtg gacagaatca gggcgctgga
      661 ttttctttcc cgcaaagcct atttcacaag tcagacacaa ttgaatacgg gtgcacggct
      721 gcttttcggt gctgctgttg tgtttctgat ttcgatgcgt cttgccgcaa gatggaatcc
      781 gaaaccgccc gggccgcctc ttcagtctgc caccaaagga cattggggcc gtcaggcact
      841 gatccgtgag ttgattattc tgtccgccat ggtgctcatg atcgcagcag ccctggcggc
      901 gctgttcaca cctctggaat ttccgcctct tgaacagcac gctcagacat ccgccgtgga
      961 agcagcaggc gataccgatg cgcctgaaac cgccgccgtt acttttcctg tctgggaaga
     1021 cctgcaaaaa cagtggcctt cttttcgcgg tcccggcggt tacggtctgg cgcatgcaga
     1081 gtcagtgccg ctgaagtggg acggtgaaag cggaaaaaat atcagatgga aaacggcgct
     1141 ggatgccccg ggtttcaatt cgccggtggt ttggggagac tccctgtttt tcagcagcgc
     1201 tgatgagaca aagcggacag ttcactgcta cgataccgat tccggcgaac tcaaatggat
     1261 gcaggtgctg ccttcttttc ccggaactcc tgagacgcct ccgaaagtaa gtgccgatac
     1321 cggttatgcg gcgccgacca tggttgcgca ctatccttat gtttatgcca tttttgccaa
     1381 tggcgatctt gcctgctacg atttttccgg aaagctgatc tgggggaaaa atctgggtgt
     1441 gccggaaaac cattacgggc atgcgtcatc gcttattgcc tgggaaaatc tcctttatat
     1501 ccagtacgac cagaagaaaa atccccggct tctggcgctg aaagcggaca caggtgcgcc
     1561 tgcgtgggaa gtgcagcgca aaaagatttc ctgggcatcg ccgatcatta ttcagcgcac
     1621 gtccggtccg cagttgattt tggcttctga aaaagacgtg gatgcttaca gcctctcaga
     1681 cggcaccctg ctctggactg ttcagtgtct ggacggtgag gtcgcgccat ctcccgcata
     1741 tacccgtgac cgtgtttttg tggggcagga ttatgcggcc gccacagcaa ttcttcttca
     1801 tgacgaggag agcagcacca cagctgaagc gacctgggaa tgggacgaca gcctcaccga
     1861 tatcgccagc cctttaggca ttggcgatta ctttattatg gcaacgtcca gagg
//
LOCUS       DAOPWB010000261         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_66235_length_1900_cov_3.147425, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000261 DAOPWB010000000
VERSION     DAOPWB010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66235_length_1900_cov_3.147425"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PKN92_13485"
     CDS             complement(<1..822)
                     /locus_tag="PKN92_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49850.1"
                     /translation="MKSIHIDIETFSSADLAKGGVYRYVESPDFEILLFGYSIDDGEV
                     EVVDLACGERIPEEIIEAIKGKDVIKTAHNANFERVCLSRYLGMPTGDYLDPSSWRCT
                     MVWSAYMGLPLSLAGVGTVLGLEKQKLEEGKELIRFFCTPRTPTKNNPATRNYPQDAP
                     EKWDLFKKYNVRDVEVETQIQDTLSKFPVPDFVWEEWFIDQQINDRGVELDMVLARNA
                     IEMDTRSKEELKNRMQQITELENPNSVQQMREWLADNGLETESLGKKEVKAMANEA"
     gene            complement(878..1021)
                     /locus_tag="PKN92_13490"
     CDS             complement(878..1021)
                     /locus_tag="PKN92_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49851.1"
                     /translation="MTHAQEMMLAICCGVCAGQLIGQILGAVVQGILILRDKRKKKKA
                     NNE"
     gene            complement(1018..1539)
                     /locus_tag="PKN92_13495"
     CDS             complement(1018..1539)
                     /locus_tag="PKN92_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49852.1"
                     /translation="MSIIEKYMHNVPWLESKMSFIRNGARLVENGICLNFGTPIFHAT
                     IEEHIKYKNTGHLKIRSIPSGNVIWCVYDEEMNEVGEYRSEEEARKAMPENGDILEID
                     LDEMFEIVDEYGKNITGMLYPTENRAWVFLYGILNPEVWSGYIAACILLELFDMVEDK
                     KKAKTEEKTEEIK"
     gene            complement(1606..>1900)
                     /locus_tag="PKN92_13500"
     CDS             complement(1606..>1900)
                     /locus_tag="PKN92_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HNZ49853.1"
                     /translation="ERPDDEAYAGCWFVNANNTAAPGIVDADRQPILDRSEVYSGVYG
                     RASISFYAFNSSGNKGIACSLNNLQKIADGEPLGGHASAEADFATDEDDDFLS"
BASE COUNT          424 a          514 c          402 g          560 t
ORIGIN      
        1 tgcctcgttt gccattgctt tgacttcctt tttcccgaga ctttctgtct ccagcccgtt
       61 gtccgcaagc cactcacgca tctgctgtac cgaattggga ttttccaatt cagtaatctg
      121 ctgcattctg tttttcagtt cttctttact tctggtatcc atctcgatgg cattgcgggc
      181 caggaccata tccagctcca caccccggtc attgatctgc tggtcaatga accattcttc
      241 ccagacaaag tccggcacag gaaatttgga gagagtatcc tgtatctgcg tttccacttc
      301 cacgtcacga acgttatatt ttttgaagag gtcccatttc tccggtgcat cctgcggata
      361 gtttcttgtg gcagggttat ttttcgttgg cgttctcggg gtgcagaaga agcggatcag
      421 ttcttttccc tcttctaatt tctgcttttc aagccccagc accgttccga caccggcaag
      481 cgaaagcggc agccccatat aggccgacca caccatggtg catcgccagg atgaagggtc
      541 aaggtaatcg cccgtaggca tcccaaggta gcgggataaa cagacccgct cgaagtttgc
      601 gttgtgggca gtctttataa cgtcctttcc ttttattgct tctatgattt cctccggtat
      661 ccgctctccg caggcaaggt ctacaacttc aacctcgccg tcatcaatgg agtaaccgaa
      721 cagcagtatt tcaaaatccg gagactcgac atagcgataa accccgccct tcgccaggtc
      781 tgcggatgaa aatgtctcta tatctatatg tattgatttc atttgaacct ccataacggc
      841 ataaagggtg gcccgatact ccgaaccacc ctgcctttta ctcgttattt gccttcttct
      901 tttttctctt atctctcaga atcagtattc cctgaacaac tgcccctaag atttgcccga
      961 tcaactgtcc ggcgcacaca ccgcagcaga tcgccagcat catttcctgt gcatgtgtca
     1021 tttgatttcc tccgtctttt cttcggtttt tgcttttttc ttatcctcaa ccatgtcgaa
     1081 cagctcaaga agtatgcagg cagcaatata gccgctccag acctccgggt ttaagatgcc
     1141 gtacagaaat acccacgcac gattttccgt aggatagagc atgccggtga tgtttttccc
     1201 gtattcatcg acgatctcaa acatttcatc aaggtcaatt tcaaggatat cgccgttttc
     1261 aggcatcgct tttctggctt cctcttcgga gcggtattcc ccgacttcat tcatttcctc
     1321 gtcatagacg caccagataa cattgccgga cggaatactg cggattttta gatgacctgt
     1381 gtttttgtac ttgatatgct cttcgatagt cgcatgaaaa atcggtgtac cgaaatttaa
     1441 gcagatgcca ttttcgacca gtcttgcacc gttgcgaata aagctcattt tgctttcaag
     1501 ccatggaacg ttgtgcatat atttttcgat aatactcata atcaattttt cctttcataa
     1561 tgtggggcag caggttttac cccaccaccc ctgtttggca cttccttagc tcaggaagtc
     1621 gtcatcctcg tccgttgcaa aatctgcctc agcagaagca tgtccgccga gaggttcacc
     1681 gtctgcaatc ttctggagat tgttaaggga acaggcgata cctttattac cggagctgtt
     1741 gaaagcgtag aaggagatag acgctctgcc gtaaactccg gaatagacct cgctgcggtc
     1801 gagaataggc tggcgatcag catctacaat accgggagcc gccgtgttgt tcgcatttac
     1861 gaaccagcag cctgcatagg cttcatcatc aggacgctcg
//
LOCUS       DAOPWB010000262         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_67633_length_1874_cov_3.282573, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000262 DAOPWB010000000
VERSION     DAOPWB010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67633_length_1874_cov_3.282573"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..>1874)
                     /gene="recG"
                     /locus_tag="PKN92_13505"
     CDS             complement(181..>1874)
                     /gene="recG"
                     /locus_tag="PKN92_13505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNZ49854.1"
                     /translation="KGLTLQNPEYEVLAEEEEGDPLNTGRIVPVYGLTAGITQRLLRR
                     WIRTALDAISAGTEESLPEQLRKKHALMPLADALAEVHFPGSLDGAQAARRRLVYEEL
                     LVMQCAILQRRALQEAEVQGVAHRTDGPLLQRLRAELPFTLTDGQQHAVKAIFDDMRA
                     KRPMFRLVQGDVGCGKTLVALHALTAAVDSGVQVAFMAPTEILAEQHYATLSPRLAPL
                     GIEAALLTASTPRAAQLRERIERGEVPVVIGTHALVQPSTRFHKLGLVIIDEQHRFGV
                     GQRDELMRKGDCPDILHLTATPIPRTLALTLYGGMDITLIPDMPPGRLPVKTTVIPET
                     RKEELYAYLCSQAAEGWQSYIVCPLVEESEHFASLTPLIDHYTALTEGALAEVRCAML
                     HGRMDPREKEEVMTAFKNREISILFSTTVIEVGVDSPAATTMVIEDAGRFGLAQLHQL
                     RGRVGRGNKQSYCFLLGASTTPEGRQRIKLLCETTDGFQIAEADLALRGPGDYCGARQ
                     SGLPDFRAADLLQDIQLLEEAREDAEALLKADPALNLREHAALKEKMARHTAIFI"
BASE COUNT          367 a          580 c          517 g          410 t
ORIGIN      
        1 tcgtgtgtag gactgatgga ggaggtatgt cccgggacat tactccgact cagattcaag
       61 tgcctgtctt tttcatggcc cgcttgtgtg ccaataggtc atcggcgttt ccgggaagaa
      121 gaattgcaca cgccgggcgt catcatctct gcgcccgtca catccgcccc cttgtatcat
      181 tcagataaaa atagcggtat gacgcgccat tttttctttc agcgcggcgt gctctctcag
      241 gttcagtgcg ggatccgcct taagcagtgc ctctgcatct tcccgtgctt cttctagcag
      301 ctgtatatcc tgaagcagat cggctgcccg aaaatcaggg agtccggact gtcgtgcacc
      361 gcagtaatct ccgggaccgc gcagcgccag atcggcctcg gcaatctgga agccgtctgt
      421 agtttcgcac aggagcttta ttcgctgcct gccttcaggc gttgtcgatg caccgaggag
      481 gaaacaataa gactgtttgt ttcccctgcc gactctgccc cggagctggt gcagctgtgc
      541 cagcccgaaa cgtccggcat cctctatcac catggtggtt gctgcaggcg aatcgacccc
      601 cacttcaatg actgttgtcg aaaaaagaat gctgatttcc cggtttttga aagccgtcat
      661 gacttcctct ttctcccggg gatccatgcg tccgtgcagc atggcacagc gcacctctgc
      721 cagcgcccct tctgtcagcg ccgtataatg atcaatcagc ggggtcagcg atgcgaaatg
      781 ttccgactcc tccacgaggg ggcagacaat atagctctgc cacccttccg ccgcctgact
      841 gcacagatag gcatacagct cctctttgcg tgtttcggga ataacagttg tttttacggg
      901 cagccttccc ggaggcatat ccgggataag ggtgatatcc atgccgccgt aaagagtcaa
      961 tgccagggtc cggggtattg gcgtggcggt gaggtgaaga atatccgggc agtctccctt
     1021 gcgcatgagc tcatcccgct ggcccacacc gaagcggtgc tgctcatcta taatcacaag
     1081 cccgagtttg tggaaacgcg tggacggctg cacgagtgca tgggttccta tgacgacggg
     1141 tacctcacct ctttcgatac gctcacggag ctgtgcggca cggggcgttg acgctgtaag
     1201 cagcgccgcc tcaataccca gcggcgcaag ccggggcgac agcgtcgcat aatgctgttc
     1261 cgccagaatt tccgtaggcg ccatgaaggc gacctgaacc cccgagtcca ctgccgccgt
     1321 aagcgcatga agggcgacca gcgttttgcc gcagcccaca tcaccctgca ccagccggaa
     1381 catggggcgc ttcgcccgca tgtcgtcaaa aatggctttt acagcatgct gctgcccgtc
     1441 ggtcaaagta aaaggaagct ccgcgcgaag ccgctgcagc aaaggcccgt ccgttctgtg
     1501 cgcaacgccc tgcacctctg cttcctgcag agcacggcgc tgcagaatgg cacactgcat
     1561 caccagcagc tcctcgtata ccagccgacg ccgggcagcc tgagcaccat ccagcgaccc
     1621 gggaaaatgg acttccgcaa gcgcatcagc aagaggcatc agcgcatgct ttttccgcag
     1681 ctgttcgggc aggctttctt ccgtcccggc actgattgca tccagtgccg tgcggatcca
     1741 tcgccgaagg aggcgttggg taatgcctgc ggtgagcccg tagacaggca caatccgccc
     1801 tgtattcagc ggatcgccct cttcctcttc cgccagcact tcgtattccg ggttctgcag
     1861 tgtgagcccc ttgt
//
LOCUS       DAOPWB010000263         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_67805_length_1871_cov_5.155286, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000263 DAOPWB010000000
VERSION     DAOPWB010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67805_length_1871_cov_5.155286"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1703
                     /locus_tag="PKN92_13510"
     CDS             132..1703
                     /locus_tag="PKN92_13510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNZ49855.1"
                     /translation="MLKRETMMSALDDRGLVWDFIIIGGGATGVGVAIDAATRGYRCL
                     LLEASDFGKGTSSRSTKLVHGGVRYLQQGNVSLVLDALRERGLMRQNAPHLVHDLAFI
                     VPNYSWWEAPFYGIGLRVYDFMAGKLGFGRSRNLSYEKTIEHIPTLETEGLRGGVIYY
                     DGQFDDARLLINMAQTAEEHGATLLNYMKVISLIKEDDLVKGVVAVDHESGQEYTLHA
                     RAVINATGPFADAVRHMDTPDAPAMISPSQGVHISLDRSFLPGDTAVMVPHTDDGRVL
                     FAIPWHNHTLIGTTDTSIPEVVEEPSVMAEEMDFLLTHAARYLTKDPSETDVLSAFTG
                     IRPLVAAQGESKTASLSRDHTLHISNSGLITITGGKWTTYRKMAEDTVNQAALVAELE
                     DRPCVTRNLPIHGHLLNSQYEGNLAVYGADACRLLELIRTNNAWEHLLHPDLNIQAGE
                     VIWAVRNEAARTVEDFLGRRSRALFLNARAAIDMAPKVAELMAAELGRDAAWKESQIR
                     ALEKIARRYYLYPAE"
BASE COUNT          440 a          515 c          502 g          414 t
ORIGIN      
        1 attggcacac aagcgtgctt cttcttctcc gcttggcttg gctttctttt tcttattcgt
       61 tacaataaag aaaatgcgga taaagcatag atgctgcttc agtacatata cagccgggag
      121 gttttcagat catgctgaaa agagaaacga tgatgtcggc tctggatgat aggggcttag
      181 tatgggactt tatcatcatt ggcggcggtg ccacgggcgt aggcgttgcc attgacgcgg
      241 caacacgggg ctaccgctgt ctgcttctgg aagcgtccga cttcggcaaa ggcacctcca
      301 gcagaagtac caagctggta cacggcggtg tacgctacct gcagcaggga aatgtatccc
      361 tggtgctgga tgcgctccgt gaaagagggc tcatgcgtca gaatgccccg catctcgtgc
      421 acgatctcgc ttttatcgtg cccaactact cctggtggga agcacctttc tacggtatcg
      481 gcctgcgtgt ttatgatttc atggcgggca aacttggatt cggacgttcc cggaatctct
      541 cctacgaaaa aaccattgaa cacatcccca ccctggaaac cgaaggcttg cgcggaggcg
      601 ttatttatta tgacggtcag tttgacgatg cccggctcct cataaacatg gcgcagacgg
      661 cagaagaaca tggtgccacg ctgctgaact atatgaaagt catcagcctg attaaagagg
      721 atgatcttgt aaaaggtgtt gtcgccgtgg atcatgaaag cgggcaggaa tatacgctcc
      781 atgcccgggc tgtcatcaat gcgacaggcc cctttgccga tgcggtcagg cacatggata
      841 cgcccgatgc tcctgccatg atcagcccga gccagggcgt gcacatctcc ctggatcgct
      901 cctttctgcc cggtgatact gctgtcatgg tgcctcatac cgacgacggc cgagtcctct
      961 ttgcgatccc ctggcataac cataccctga tcggtactac ggacacgagc attcccgaag
     1021 tggtggaaga accgtcggtc atggcggagg aaatggattt tctgctgacc catgccgcac
     1081 ggtatctgac caaagatcct tctgaaacgg atgtgctgag cgcattcacc ggaatccggc
     1141 ctcttgtcgc cgcacagggc gaaagtaaaa cggcatccct ctccagagac cataccctgc
     1201 atatttccaa ttcagggctg atcaccatta caggcggaaa atggaccaca tacagaaaga
     1261 tggcggaaga taccgtcaat caggctgcgc ttgtggcgga gctcgaggac aggccctgcg
     1321 tcacccgcaa cctcccgata cacggacatc tgctcaattc ccagtatgag ggcaaccttg
     1381 ccgtctacgg cgctgatgcc tgcaggcttc tggagctgat ccggacaaat aacgcctggg
     1441 agcaccttct tcatcccgat ctgaatattc aggcgggaga agtgatctgg gcagtgcgca
     1501 acgaggcggc gcgtactgtt gaagattttc tcggacgccg atcccgggca ctctttctca
     1561 atgcccgggc tgccatcgat atggcaccga aagttgcaga acttatggcg gcggaactgg
     1621 gcagagatgc ggcatggaag gaaagccaga ttcgcgcact cgagaaaata gcacgccgct
     1681 attacctgta tccggcagaa taaagcacag tcacattcaa tccgcctcag gcggaaatct
     1741 tctttctctt ttccggctct ccgaagggat acagcgcatc gctcactgca ggtgccgggg
     1801 cgttaccgcg gtttatcaag tgccatacag caaaccatac cagactgatt acaataacac
     1861 ccagggcaac g
//
LOCUS       DAOPWB010000264         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_68615_length_1857_cov_4.431188, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000264 DAOPWB010000000
VERSION     DAOPWB010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68615_length_1857_cov_4.431188"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="PKN92_13515"
     CDS             complement(<1..1822)
                     /locus_tag="PKN92_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNZ49856.1"
                     /translation="MIFDPATVDAILDRTGKGADALIPVLHALQAKYKYLPDEALRYL
                     CECSEITPAVVESVVSFFPGFRRRPTGKHTISVCDGTACHVKGAPGVYDAVAEALKLK
                     PGEDTDAGGNFTLRKVACLGCCTLAPAVQIDGVTYGHVQTDAVDIMLADFLKNADLPK
                     NAVPEAAAGPEKTEGEIRIGLGSCCVAGGSAKVRAALLKTLHELGATIPVKNVSCVGI
                     CHQTPLLEIILPGKPPALYAKVQAEDVESIIVRHIPAQKAFHSWKRKAARWFEWMYIS
                     DSIKKPQEYALDAEGASMTAFLKSQRRLATEYCGEIDPLDLEEYRRRGGFQALQTCLQ
                     SMHALDADGAVPFYEKILAEIEASGLRGRGGAGFPVAAKWRAVRASGDTKKYVICNGD
                     EGDPGAFMDRMMLESYPFRVIEGMLIAALAVNAQEGIFYIRAEYPLAVERVNRAIALC
                     EKGGLLGKTVAGTSFSFRIRVVQGAGAFICGEETALIASVEGRRGTPTLRPPYPAERG
                     VFGCPTLVNNTETLALVPWIMRNGAAAFAALGTPKSTGTKVFSLAGKVVRGGLIEVPM
                     GMSISAIVEEIGGGVADGRKLKAVQIGGPSGGCIPASLAQT"
BASE COUNT          405 a          585 c          456 g          411 t
ORIGIN      
        1 gggtctgtgc cagtgatgca ggaatgcacc cgcccgaagg cccgccaatc tgcacggctt
       61 tcagctttct gccgtccgcc acgccgccgc cgatttcctc aacaatggcg ctgatcgaca
      121 tacccatggg cacttcgatc aatccgccgc gcaccacttt tcctgccaga gaaaacactt
      181 tggttccagt gctttttggg gtgccgagtg ctgcaaaagc agccgcaccg ttgcgcataa
      241 tccagggtac cagcgccagc gtctccgtat tgttgacaag ggtcggacag ccgaagacac
      301 cccgctctgc cggatagggt ggccgcagag tcggcgttcc ccgccttccc tcgaccgaag
      361 cgatcagcgc tgtttcttcc ccgcagatga aagcacctgc tccctgtaca acccggatgc
      421 ggaaagaaaa ggacgttcca gcgaccgttt tgccgagaag accgcctttt tcacacaggg
      481 caatggcgcg gttcacccgc tcaactgcaa gagggtattc tgctctgatg tagaaaatac
      541 cttcctgcgc attgaccgcc agagcggcaa tcagcatgcc ctcgatcaca cgaaaaggat
      601 atgattccag catcatgcga tccataaatg cacccgggtc accttcgtct ccattgcaga
      661 tgacatattt tttcgtgtcg ccggatgcac ggacagcacg ccatttcgcc gcaacaggaa
      721 aaccggcgcc accacgcccg cgcaaaccgc tcgcttctat ctccgccagt atcttttcgt
      781 aaaacgggac agcgccatct gcatccaggg catgcatgga ctgaagacat gtctgcaaag
      841 cctgaaatcc gccccggcgt ctgtattcct ccagatcaag cggatcgatc tcaccgcagt
      901 actccgtagc caggcggcgc tgcgacttga ggaaagcagt catggacgcc ccctccgcat
      961 ccagagcata ttcctgcggc tttttaatgc tgtccgagat gtacatccat tcaaaccagc
     1021 gcgccgcctt tcttttccag gaatgaaagg ccttttgcgc cggtatatgc cggacgataa
     1081 tcgactcaac gtcctctgcc tgcacctttg catacagtgc aggcggttta ccgggaagaa
     1141 taatttccaa tagaggtgtc tgatggcata tgccgacaca gctcacattt tttacgggaa
     1201 ttgtggcgcc cagttcatga agcgtcttca gcaaagcggc acgcactttt gcactgccgc
     1261 cggcaacgca gcaggaaccg agccctattc tgatttcccc ttctgttttt tctggtccgg
     1321 ccgctgcttc aggcactgcg tttttcggca ggtcagcatt ttttaaaaaa tccgccagca
     1381 tgatgtccac agcatccgtc tgcacatgcc cgtaggttac cccgtcgatc tgcactgccg
     1441 gcgccagcgt acagcatccc aggcacgcca ctttacgcaa cgtgaaattt ccgcccgcat
     1501 ccgtatcttc tccgggcttc aatttcagcg cttccgccac cgcatcataa acacccggcg
     1561 cccctttgac gtggcaggct gtgccgtcac aaacactgat ggtatgtttg cctgtcgggc
     1621 ggcgccggaa gcccgggaaa aaggacacca cactttccac aacggcgggt gtaatttcgg
     1681 agcattcgca cagatagcgc agggcttcat caggcaggta tttgtatttt gcctgcagtg
     1741 catggagcac cggaatcagt gcatcagcac cttttccggt tctgtcgaga atggcatcta
     1801 cagtggcggg atcaaaaatc attgttccgc cccttcctcc acacaccaaa gataacc
//
LOCUS       DAOPWB010000265         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_69914_length_1835_cov_5.958989, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000265 DAOPWB010000000
VERSION     DAOPWB010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69914_length_1835_cov_5.958989"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..389
                     /locus_tag="PKN92_13520"
     CDS             18..389
                     /locus_tag="PKN92_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNZ49857.1"
                     /translation="MEFDRWSNKVIGCAVEVHRHLGPGLLESVYSKCLAHEMELCGLH
                     FTRETSLPVCYKGMTIEGGYRVDFLVEDELIVELKSTETILPLYQAQLLSYLKPSGKN
                     TGLIINFNVPLLKAGIKRMVL"
     gene            826..1323
                     /locus_tag="PKN92_13525"
     CDS             826..1323
                     /locus_tag="PKN92_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49858.1"
                     /translation="MKKMTMVMVAMAMVAGIAFASSLSIPWFVDNAPVGANWPPDTGL
                     MTLIYLKSNVTDVLQCEITYFSEDGVNLGPVYPANTFTIAPQSAVAFRPVALDPIGSL
                     PGGSTLGVEGAQGVAVPDRPRDVDTKKNGSASISWVGGPNDLQGMVATTSMAMSYAHL
                     LPPGN"
     gene            1498..>1835
                     /locus_tag="PKN92_13530"
     CDS             1498..>1835
                     /locus_tag="PKN92_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49859.1"
                     /translation="MSSAGKDTPDFSRNARCLACASKSLHFFGTRDRHRYDRCGHCGT
                     LQLNPMPSAAFLAEAYSKNYAVAGHCQGDPAIRNKAAVPQYDALCDALGRSGVRGRIL
                     DHGCGWGGLLL"
BASE COUNT          450 a          400 c          468 g          517 t
ORIGIN      
        1 agagaaggga agggaaaatg gaatttgatc gatggtcaaa taaagtaatc ggttgtgctg
       61 tagaagttca caggcattta ggacctggac tgcttgaaag tgtttattca aaatgtttgg
      121 cacatgagat ggagctgtgc gggcttcatt ttacaagaga gacttcgctc cctgtttgct
      181 ataaagggat gactatagag ggagggtatc gtgtagattt tttagtcgaa gatgaattga
      241 ttgttgaatt gaaaagtaca gaaacgattc tgcctttgta tcaggctcaa ttgcttagct
      301 atttaaaacc gtccggaaaa aacaccggat tgattataaa ttttaatgtc cctttattaa
      361 aagctggcat aaaaagaatg gtgctctgaa caacaagtag ttttcttcat gaatcttcgt
      421 gcccttcgtg ccttcgtggc tatctttcca tgcttttgcg gttaaagtgc cacccatttt
      481 aaaaaaactt actcagcaat gcagcttttt ccgttatttt atgttttccc ctgtttttag
      541 attcgtcctg gaagcacacc gccgctctaa tcggtaaatg aaagaaaagc ctttaaacac
      601 agaacaaaaa agtcttttcc ctccccgata tcacgttgtg ttttttgcgt gaaaaagaat
      661 tcgatctttt ttaaaaataa agcttgacaa aacgaactgg tttgtggtac aaactagttg
      721 gagttagtaa ggacgctgac tcacacaaaa gttgaaaaga ctgtagacgg cttagtcgcc
      781 gatataaaga ctacagaatt ttcagaagac aggaggtgaa aaagtatgaa aaagatgaca
      841 atggttatgg ttgctatggc catggtggca gggattgctt ttgcgagctc tctttctatt
      901 ccctggttcg tagacaacgc tcccgtcggt gccaactggc cgccggatac cggtctgatg
      961 acccttattt acctgaagag caatgtgact gatgtgctgc agtgcgaaat cacctatttc
     1021 tctgaagatg gtgtaaatct gggacccgtc tatccggcca atacgttcac cattgcgccg
     1081 cagtccgccg tggctttccg cccggtagcg ctggatccca tcggatcgct tcctggcggt
     1141 tctacactgg gtgtggaagg tgctcagggt gttgcagttc ccgaccgtcc gcgtgatgtc
     1201 gacacgaaaa agaacggttc cgccagcatc agctgggtgg gtggaccgaa cgaccttcag
     1261 ggtatggtcg ccacgacgag catggcaatg tcctatgctc acctgctgcc tcccggaaat
     1321 tgattccagg actcttaaca tctattgttt gacttgatag gttgaggcga gaatgccgta
     1381 gggcatgctc gcctctttct ttttttacac ggggcggcat tggtctttgc tgcactgttg
     1441 ggataaaata aggggcgaat cggtattttc cttctttttc ccaggaaaga aaagagcatg
     1501 tcttctgccg gcaaagatac accggatttc tcccggaacg cccgttgcct tgcctgtgct
     1561 tcaaaatcac ttcatttttt cggcacgcgg gacaggcacc gttatgaccg ttgcggccac
     1621 tgcggcaccc tccagttgaa tcccatgcct tccgccgctt ttcttgcaga agcgtattca
     1681 aaaaactatg cggtggcggg gcattgtcag ggagatccgg caatacggaa taaggcggct
     1741 gttcctcagt acgatgctct ttgtgatgcg ctggggcggt ctggcgtgcg gggaaggatt
     1801 ctggatcatg gttgtggctg gggcgggctt ctgct
//
LOCUS       DAOPWB010000266         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_72094_length_1800_cov_4.734097, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000266 DAOPWB010000000
VERSION     DAOPWB010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72094_length_1800_cov_4.734097"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PKN92_13535"
     CDS             <1..764
                     /locus_tag="PKN92_13535"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNZ49860.1"
                     /translation="ILKPGDSFSDELKDAFKVVLPDEGMWGGYAPPYSQMLIDAGKDK
                     LKRQMGWMTRNQPLTLDSGRILLPLYNDGFNISIMAYSDDLGDNWHAGAPIVGLGPIQ
                     PTLVRKNDGTLFAYFRDSGGEPQRIQQAHSEDDGIHWSLTSDTDIPNPSSSMELQRLD
                     DGRWILIFNDTEDGRHRLAVALSKDEGKSWPDKRYLEQAESKQDGSFGYPSIIQAKDG
                     TIHATYSYKTKEGAAIKHAAFSPDWITSGKVRKSR"
     gene            complement(775..>1800)
                     /locus_tag="PKN92_13540"
     CDS             complement(775..>1800)
                     /locus_tag="PKN92_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNZ49861.1"
                     /translation="LGVVLDIAAWNYPLLIAVNVVVPAVLAGNAVIVKHSSRTPLAGQ
                     MFQDLFRDAGAPPNLVQNVIADHAVTAMLIQHKQVDHVSFTGSVKGGHEVMAASAGRF
                     IDVGLELGGKDPAYVCADADFDYAVDNCVDGAFYNAGQSCCAIERIYVERSIYDRFVE
                     AYAAKAREYVLGDPMASGTSMGPLAGKSAAALLEKQVADAVAAGGRLVVSSDEFARPD
                     QGWFFGPAVVADAPQHCALMQEESFGPVIGITPVSGDEEAIQYMNDSAYGLTASIWTS
                     DFARAVRIGEQIATGTFYMNRCDYLDPALPWCGVKDTGRGATLSHHGFLHLTRLKSMH
                     LRTDIPT"
BASE COUNT          464 a          517 c          465 g          354 t
ORIGIN      
        1 ttatccttaa accgggcgac tctttttccg atgaattgaa agacgcgttt aaagtggtgc
       61 tccccgatga aggcatgtgg ggcggttatg cgccgccgta tagccaaatg ctgattgacg
      121 cgggaaaaga caaattgaaa cgccaaatgg gctggatgac aagaaaccag ccgctcacac
      181 tggactcggg cagaatcctc ctccctcttt ataacgacgg gttcaacatt tccatcatgg
      241 cgtactccga tgatctcggc gacaactggc acgccggggc gcccattgta ggactcgggc
      301 ctattcagcc aactcttgtc agaaaaaatg acggaacgct ttttgcctac ttccgagaca
      361 gcggcggaga gcctcagcgt atccaacagg cgcattccga ggatgacggc attcactggt
      421 cgctgaccag cgacacagac atacccaacc cgagttccag tatggagctc caaaggcttg
      481 acgacggtcg ctggatcctg atctttaacg atacggaaga cggtcggcac aggctagccg
      541 tcgccctttc aaaagatgaa gggaaaagct ggccggataa aagatatctg gagcaggcgg
      601 aaagcaaaca ggacggatct ttcggctatc cctccatcat ccaggcgaaa gacggcacaa
      661 tacacgccac ctattcgtac aagaccaaag aaggcgccgc tattaaacac gccgcctttt
      721 cgcccgactg gatcacaagc ggcaaagtgc ggaaaagccg ctgaagagtc agtttcaggt
      781 aggaatatca gtgcgcaggt gcatgctttt cagacgggtc agatgtaaaa atccgtgatg
      841 cgaaagggtg gcaccacggc ctgtgtcttt aacaccgcac cagggcagcg ccggatccag
      901 ataatcacag cggttcatat agaaagttcc ggtggcgatc tgctcaccga tgcgcaccgc
      961 ccgggcaaaa tctgacgtcc agatggatgc ggtcaatccg tatgcactgt cgttcatata
     1021 ctgtatcgct tcctcatcgc cggaaacggg cgttattccg atgacaggac cgaagctttc
     1081 ttcctgcatc agcgcgcagt gctgcggtgc atcagccacc actgccggtc cgaaaaacca
     1141 gccctgatcg gggcgggcaa actcatcgga agaaacaacc aatctcccgc ctgccgccac
     1201 cgcatcagcc acctgttttt ccagcagtgc cgccgcgctt tttcctgcca gcgggcccat
     1261 actggttccc gatgccatgg gatcgcccag cacatattcc cgtgctttgg cggcataggc
     1321 ttcaacaaaa cgatcataga tggatcgttc cacatagata cgttctatgg cacagcagga
     1381 ctggcccgca ttatagaatg cgccgtcaac gcagttgtca acggcgtagt caaaatcggc
     1441 atcggcacag acataagccg gatctttgcc gcccagttca aggcccacat cgataaatct
     1501 gccggcagaa gccgccatga cttcgtgccc gcctttaacc gagcccgtga aagagacatg
     1561 gtctacctgt ttatgctgaa tcagcatggc agtaaccgca tgatcagcaa tcacattctg
     1621 caccagattg ggcggtgcac cggcatcacg aaaaagatcc tgaaacatct gaccggccag
     1681 gggggtccgg ctcgagtgct ttacgattac cgcattgcct gcaaggacgg ccggaacaac
     1741 aacattcacg gcaataagaa gcggataatt ccaagcggca atatcgagca caacccccag
//
LOCUS       DAOPWB010000267         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_76861_length_1727_cov_3.007775, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000267 DAOPWB010000000
VERSION     DAOPWB010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76861_length_1727_cov_3.007775"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..662
                     /gene="cas4"
                     /locus_tag="PKN92_13545"
     CDS             30..662
                     /gene="cas4"
                     /locus_tag="PKN92_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNZ49862.1"
                     /translation="MYSEDDFIQLSALQHYAFCPRQCALIHVEQMWSENLLTAQGRLM
                     HEHVHEEGDESRGDLYIERGVALRSFTLGLIGKADVIEYHRQTDGNWLVFPVEYKRGK
                     AKPDNSDKIQLCAQAICLEEMRNVRIPAGAIFYGKTRKRLDVAFDDALRWETQDTAQK
                     THALLESGRTPGPVYGRRCENCSLIAECMPQTIQKKRSVESYLKRELDKE"
     gene            659..1690
                     /gene="cas1c"
                     /locus_tag="PKN92_13550"
     CDS             659..1690
                     /gene="cas1c"
                     /locus_tag="PKN92_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HNZ49863.1"
                     /translation="MKKLLNTLYVTTQGAYLAKEGETVAVKIDQEVRLRIPVHTIGGI
                     VCFGNVSCSPFLMGFCSENGICISFLNQHGRFLARVQGPVSGNVLLRREQYRSADNDD
                     LSGKLAKAFVTGKVANCRTVLQRALRDHSGKIDEEALRLALDNLNRTLKSLELNQTLN
                     EVRGLEGHAAHTYFSVFDHLIVAQKDEFSFQERNRRPPMDNVNCLLSFIYTLLVHDCR
                     SALESVGLDPAVGFLHRDRPGRPGLALDLMEEFRPFFADRLVLSLINLRQVQHKGFKK
                     TEAGSVVMNDETRKVLLVAYQERKQEEILHPFLEEKVSIGMLFHVQALLLARCLRGDL
                     DGYPPFIWK"
BASE COUNT          420 a          381 c          471 g          455 t
ORIGIN      
        1 aggtaataaa ttaggtaata gccggtgata tgtatagcga agacgacttc atccaacttt
       61 ccgccctgca gcattacgcc ttttgtccgc ggcaatgtgc gctgatccat gttgagcaga
      121 tgtggtctga aaatcttctc accgcccagg gacgtcttat gcatgagcat gttcatgaag
      181 aaggcgatga atcgagaggg gatctttata tagagcgtgg cgttgctttg cgctctttca
      241 ctctcggact aattggcaag gccgatgtca ttgaatacca tcgtcagacg gatggaaact
      301 ggcttgtttt ccccgtagaa tataagcggg gaaaggccaa gccggacaac agcgataaaa
      361 tacagctttg cgcacaggcc atctgtctgg aagaaatgcg caatgtgcgt attccggcag
      421 gtgctatttt ttatggcaag accagaaaac gtctggatgt agcttttgat gatgcactgc
      481 ggtgggaaac gcaagatacc gctcagaaaa ctcatgcact gcttgaatca ggccggacgc
      541 cagggccggt ttatggcagg cggtgtgaaa actgttcgct cattgctgaa tgcatgcccc
      601 aaacgattca gaagaagcgg tcagttgaaa gttatttgaa gcgagagttg gataaagaat
      661 gaaaaaactc ctcaatactt tgtatgtaac gacgcagggc gcctatctgg ccaaggaagg
      721 agagacagtg gcggtcaaaa ttgaccagga ggtgcgcctg cgtattcccg tacatacgat
      781 tggtggtatt gtatgcttcg gtaatgtatc atgcagccct tttttgatgg ggttttgcag
      841 tgaaaacggc atctgtataa gttttctgaa tcaacatggg cgttttctcg caagggtgca
      901 gggcccagtg tccggtaatg ttcttctgcg ccgtgagcag tatcgaagtg ccgacaatga
      961 tgacttgtcg ggaaaattgg caaaagcctt cgttaccggc aaggttgcta attgtcggac
     1021 cgtgcttcaa agggctctgc gggatcattc ggggaagata gacgaggaag ctttgcgtct
     1081 ggctcttgac aacctgaacc gtacgctcaa atcactggag ttaaatcaga cgctcaatga
     1141 ggtccgtggt ttggaggggc atgcggcaca tacatatttc agtgtgtttg accacttgat
     1201 cgtggcgcag aaagacgaat tttcgtttca ggagcgcaac agacgtcccc cgatggacaa
     1261 cgtcaattgc cttctttcct ttatttatac cttactcgtg catgactgcc gtagcgcgct
     1321 ggaatcggtc ggtctggatc cggccgtcgg tttcctgcat cgtgatcggc cgggccgacc
     1381 aggccttgca ttggatttaa tggaagagtt tcgcccgttt tttgcggatc gtctcgtttt
     1441 gtcgctcata aatctgcgtc aagtgcagca caaaggattt aaaaagactg aggcaggctc
     1501 cgtcgttatg aatgacgaaa cccggaaagt gttgcttgtt gcctatcagg aaagaaaaca
     1561 agaagaaatt ttgcatccgt ttctggaaga gaaagtgtcc attggcatgc tctttcatgt
     1621 tcaggcactt ttgctggcac gctgtttgcg gggcgatttg gacggatacc ccccgtttat
     1681 ctggaagtaa atgctcactt ccggtatctc ctttgcccct ttacggg
//
LOCUS       DAOPWB010000268         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_77149_length_1723_cov_3.789568, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000268 DAOPWB010000000
VERSION     DAOPWB010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77149_length_1723_cov_3.789568"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="PKN92_13555"
     CDS             <1..1350
                     /locus_tag="PKN92_13555"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNZ49864.1"
                     /translation="ASVVMGTPFYMSPEQYKNTRDVDARADIYSVGIMLYELLTGSVP
                     TGVPKPVSEAFKDVPPVMDDLLKQCIDPDREKRFSNATEMRKALAPVIELLKQGKDLT
                     KIKTQQIYQTKKQPIVPTHLWAAIGIVLVLALTGAALWALELQNRSYADNAASPVPGF
                     LSRGRSGLDELKPLAERMRASAAALSEKNSLQQKWLEDSGTLWTQAQSMARNDPAAAQ
                     KIGLSALQHLLAVLLHPDNMVLVPAGTVQIDGTSTFVPGFLIDRREVSISDFTAFCQQ
                     VPGGWPVPDELGSGLENWSDHPVSCVSWFDAQAYAAWKGRFLPTRAQWTRAAYGDNDG
                     PQFFPWGNDWKAGVANVQTGQSAPVESFPEDLSWSGCYDLAGNLSEWLRPDPAAAQPP
                     DFGVLMPICGGNFNRQRLLYESDVRPFESRSPDQGFRCVMELPWTKEAIESEIAHIH"
     gene            complement(1344..>1723)
                     /locus_tag="PKN92_13560"
     CDS             complement(1344..>1723)
                     /locus_tag="PKN92_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNZ49865.1"
                     /translation="RAPSEGGALTYEEQSEAGTRLLARYLGLSEENWSLAYQSRFGKG
                     WLEPSTGSLLLRLLASGKKRVALIAPSFVADCLETLEELDEELRVTFLQSGGDALLRI
                     PSLNAHPAWIRGAAELICRPLPQ"
BASE COUNT          396 a          499 c          489 g          339 t
ORIGIN      
        1 gcatccgtgg tgatgggcac gcctttttat atgtccccgg aacagtacaa aaacacacgc
       61 gacgttgatg cccgggcaga catttacagc gtcggaatca tgctctatga actgctgacg
      121 ggcagtgttc ccacaggggt gccgaaaccg gtttctgaag cttttaagga cgtcccgcct
      181 gtcatggatg acctgctgaa acaatgcatc gatcctgatc gggaaaagcg cttcagcaat
      241 gccacagaga tgcgcaaagc actggcgccg gtcatcgagc tgctgaagca gggcaaagat
      301 ctgactaaaa tcaaaacgca gcaaatctac cagacaaaaa agcagcctat agtgccgacg
      361 cacctttggg cggctatcgg catcgtgctg gtgctcgccc ttaccggtgc cgctttgtgg
      421 gcacttgaac ttcaaaatcg atcctatgcc gacaacgcgg cgtctcctgt tcccggcttt
      481 ctttcccggg ggcgcagcgg gcttgatgaa ctcaaaccgc tggcggaacg gatgcgggca
      541 agcgccgccg cactttcaga aaaaaacagc cttcagcaga agtggctgga agacagcggc
      601 accctctgga cacaggcaca aagcatggcg cgcaacgatc ccgccgcagc gcagaagatc
      661 ggactcagcg cgttgcagca cctgctcgcc gtactgcttc accccgacaa tatggtgctg
      721 gtgcctgcag gcactgtaca gattgacggg acgagtactt ttgtacccgg cttcctcatt
      781 gaccggcggg aagtgagtat cagcgacttc accgccttct gccagcaggt gcccggaggc
      841 tggcctgtcc ctgacgaact gggcagcggc ctggaaaact ggtccgatca tcccgtgtcc
      901 tgtgtgtcct ggtttgacgc acaggcctat gccgcctgga aaggacgctt tttacccacc
      961 cgggcgcaat ggacacgggc cgcctatggc gacaacgacg gcccacagtt ctttccctgg
     1021 ggaaatgact ggaaggccgg tgtcgccaat gtgcagacag ggcaaagcgc accggtggaa
     1081 agttttcctg aagatctgag ctggtcaggc tgttatgatc ttgcgggaaa cctctcggaa
     1141 tggctgcggc ctgacccggc agcggcacag ccgcccgatt tcggagtact gatgcccatt
     1201 tgcggcggca atttcaaccg ccagcgcttg ctctatgaaa gcgatgtacg gccctttgaa
     1261 agcagaagcc cggatcaggg tttccgctgt gtgatggaac tgccctggac caaagaagct
     1321 atcgaatcgg agatcgcaca cattcactga ggcaacggac ggcaaatcaa ctctgccgcc
     1381 ccgcggatcc aggccgggtg ggcgttcagt gagggtatgc gcagcagggc atcaccgcct
     1441 gactgaagaa aagtgacgcg aagctcttca tccagttctt caagcgtttc aagacaatcc
     1501 gccacaaaag aaggtgctat cagtgccact ctttttttgc ccgatgccaa aagacgaagg
     1561 agaaggctcc cggtggaagg ctcaagccat cctttgccga agcgggactg ataggcaaga
     1621 gaccagttct cttccgaaag tcccagatac cttgcaagaa gacgggttcc cgcctcactc
     1681 tgctcttcgt aagtgagagc ccctccttct gaaggtgccc gat
//
LOCUS       DAOPWB010000269         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_87523_length_1590_cov_4.289902, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000269 DAOPWB010000000
VERSION     DAOPWB010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87523_length_1590_cov_4.289902"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKN92_13565"
     CDS             <1..633
                     /locus_tag="PKN92_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49866.1"
                     /translation="LIMAREALDTHFSLAMPILKRKPGGPSLIKLVAKLLAFYSTWYP
                     TTYLPAKRNFNVKKLNAANRAHLKFASRTCKKLARKIFHTMALYGPKLEYEQIILGNF
                     VDIGTDLFVMCASLAYAEKLLTENPEDQTPQALVDLFCSEARRRIKANFKQARCNFNK
                     KYVKVAKLLMDGKLRWLAKDALTDIPPKYQDYAEQGYERPETKLTAKKEG"
     gene            complement(793..>1590)
                     /locus_tag="PKN92_13570"
     CDS             complement(793..>1590)
                     /locus_tag="PKN92_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49867.1"
                     /translation="RPLRHDLATLMLPHLPQKPAIRILFAESIVDDSPESGPVFEDGE
                     KILRERFSDFGFTVKGIHDLLDQYDMEFLAQVTGGERESGASFARSCLEEVVIVGASQ
                     VFQEAVAPGSNMLRSRAETTLRVFSGQDGKLQDTLYAQAVVQSADPGEGALQAVQDSC
                     AKLAGECIVAAILSGTGQLNGQQVIVTVEQPQEEERITLLLNFLQTLEGVEQATLLFY
                     APGQARLRLLYPGSMASLADMLTGIRLQDKRAEVTRCVGRELTLSLF"
BASE COUNT          393 a          422 c          426 g          349 t
ORIGIN      
        1 ctgatcatgg cgcgcgaagc actggatacc catttcagtc ttgccatgcc cattctcaaa
       61 cggaagcccg gcggacccag cctcatcaaa ctggtggcaa agctgctcgc tttctacagc
      121 acctggtatc cgacgactta cctgcctgca aagagaaact tcaatgttaa aaaactgaat
      181 gccgccaacc gtgctcacct caaatttgcc tccaggacct gcaaaaaact ggcgcggaaa
      241 attttccata ccatggcgct ttacgggccc aaactggagt acgagcagat tattctcggc
      301 aacttcgttg atatcggcac agatcttttt gtgatgtgcg cttctcttgc ctatgcggaa
      361 aaactgctga ctgaaaatcc cgaagatcag acaccgcagg cgctggttga cctcttctgc
      421 agcgaagcac gccgccggat caaagccaac ttcaagcagg cgcggtgcaa cttcaacaag
      481 aaatatgtca aagtggcgaa gctcctgatg gatggaaaac tcaggtggct tgccaaagat
      541 gcgctgacgg atattccgcc gaagtatcag gattatgcag aacaaggata tgagcggccc
      601 gagacaaaat tgactgctaa aaaagaaggc tgatgcctta ctgagcgtgt aatttccgac
      661 cgcaggagtc cctggattcc tgcggtcttt ttttgaggga gtgtgctgcg ttcaggttca
      721 cgtacgttgg cgaaaggcat gccggagtcg caaagaacag atagcgtaaa gatatcctgt
      781 gcgaagcggg ggtcagaaga ggctgagcgt cagttctctg ccgacacatc gggttacttc
      841 cgcccgttta tcttgcagcc ggataccggt gagcatgtcg gctaaggatg ccatggagcc
      901 cgggtagagc aggcggaggc gtgcctggcc gggtgcatag aaaagcaagg tggcctgctc
      961 cacgccttca agtgtctgga gaaaattaag aagtaaggta atacgctctt cttcctgagg
     1021 ttgttcgacg gtgacgatca cctgctgtcc gttcagctgt cctgttccgc tgaggatggc
     1081 ggcgacgata cattcaccgg caagtttggc acagctgtcc tgcacagcct gaagggcgcc
     1141 ctcaccggga tcggcacttt gtacaacggc ctgggcgtat aaagtatcct gcagttttcc
     1201 gtcctgccct gaaaaaacac gcaaagttgt ttccgcacgg ctgcgcagca tgttggagcc
     1261 cggtgcgacc gcttcttgaa acacctggga agcccccaca ataaccacct cttccagaca
     1321 ggatcgcgca aaggaagcgc cgctttccct ttcgccccca gtcacttgag cgagaaactc
     1381 catgtcatat tgatccagga ggtcatggat accttttacc gtgaaaccga aatcggaaaa
     1441 acgttcgcgc aggatttttt caccatcttc aaaaacaggc ccgctctccg gtgaatcgtc
     1501 gacgattgat tctgcaaaaa ggatacggat ggcaggcttt tgcggaaggt gaggcagcat
     1561 gagcgttgcc agatcgtgcc gaagcggtct
//
LOCUS       DAOPWB010000270         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Hydrogenedentota bacterium isolate
            AMR_MDS_1377 NODE_94412_length_1515_cov_4.413014, whole genome
            shotgun sequence.
ACCESSION   DAOPWB010000270 DAOPWB010000000
VERSION     DAOPWB010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854582
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Hydrogenedentota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Hydrogenedentota bacterium
            Bacteria; Candidatus Hydrogenedentota.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:10:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,716
            CDSs (total)                      :: 2,680
            Genes (coding)                    :: 2,670
            CDSs (with protein)               :: 2,670
            Genes (RNA)                       :: 36
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Candidatus Hydrogenedentota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94412_length_1515_cov_4.413014"
                     /isolate="AMR_MDS_1377"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030809"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKN92_13575"
     CDS             <1..217
                     /locus_tag="PKN92_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNZ49868.1"
                     /translation="EGAVLCALERDRYPQSGTMLAWDTLAVLMAGYESAEKGSRFIDI
                     SDYTLGRDFPLNVAPDPLLAGEPLQRH"
     gene            complement(165..1139)
                     /locus_tag="PKN92_13580"
     CDS             complement(165..1139)
                     /locus_tag="PKN92_13580"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNZ49869.1"
                     /translation="MKNLLDLFTRYFALFVLIFGILAYLFPAPFLFLKPGMNWYFALT
                     MFGIGAALELEDFKEILRTPRPVAIGVIAQFTIMPFGAFAVAKLLHLPDALLVGLVLT
                     GAAPGAMASNVMSYIAKADTAYSVSLTTVSTLLCPVLTPGLTLFLAGARMDVSFLEMF
                     SSVLFTVVVPLLLGLALKARYGVRLQPFLHFFPALSATFIIFVCTMVIALNRDSLAAM
                     TGITLAACLLLNFFGMLSGYGIGALFHLGKRRARTLSIEVGMQNAGLGAVLALKHLGR
                     ESAIPAAIFVFVCIFTASMLAAVWQRLDPDQANGAAGALPPAADPAQR"
BASE COUNT          391 a          376 c          437 g          311 t
ORIGIN      
        1 ggagggcgcc gttttgtgtg cactggagcg ggaccgctat ccccagagcg gaaccatgct
       61 tgcctgggat accctcgctg ttctgatggc aggctacgaa tcggcggaaa aaggatcgcg
      121 tttcattgac atcagcgact ataccctcgg gcgcgatttt ccccttaacg ttgcgccgga
      181 tccgctgctg gcgggagagc ccctgcagcg ccattagcct ggtcaggatc cagccgctgc
      241 cacacagccg ccagcatgct cgcggtaaaa atgcagacaa agacaaagat cgccgccgga
      301 atggctgatt ctctgccaag atgtttgagc gccagcaccg cacccagccc ggcattctgc
      361 atgcccactt caatactgag cgtacgtgcg cgccgtttgc ccagatggaa cagtgcgccg
      421 atgccgtagc cggaaagcat gccgaagaaa ttcaggagca gacatgcggc aagggtaatc
      481 ccggtcattg ctgccaggga atcccggttg agtgcaatga ccatggtgca cacgaaaata
      541 ataaaggtgg cggaaagcgc agggaagaaa tggaggaaag gctgcaggcg gactccgtac
      601 cgtgctttca gcgccaatcc gagcagcagc ggcacgacta cggtgaacag gacactgcta
      661 aacatttcaa gaaaagagac atccatccgg gcgcctgcaa gaaaaagcgt cagccccggg
      721 gtgagtacgg gacagagcag ggtggatacc gtggtcagcg atacggagta ggctgtgtcc
      781 gctttcgcaa tatagctcat tacgttgctt gccatggcgc cgggcgcagc gcctgtcagt
      841 accagaccga ccagcagcgc atcaggcaga tgcagcagct tcgccacggc aaaagcgccg
      901 aaaggcatga tggtaaattg agcgataaca ccgatcgcaa caggacgggg ggtgcgcagg
      961 atctctttaa aatcctccag ttccagtgcc gctccgatcc cgaacatggt caatgcaaaa
     1021 taccagttca tgccgggttt caggaataga aagggagcag ggaagaggta cgccagtata
     1081 ccgaagataa gcacaaacaa agcgaaatag cgggtgaaaa gatccagaag gtttttcatg
     1141 gcaggattct gtttttttaa aggcacggtt gttgcagaaa agaaaagtgt agcatgaaaa
     1201 aaggaggtct tgcataaatt ttggaaatga gaaatacttt aaactcaaaa cataaaatcc
     1261 gccagcctgg gaacgccaat ctccgcattg gctggagcgt gtgtggtcta tgttttatgg
     1321 gggtatgtcc cgagacgtat ttccgaatca gattcaagtg cctatctttt tcatgattcg
     1381 cttgtgtgcc aatgcggaga ttggcgttcc caggggaatc cgctttaggt tcgtgtggaa
     1441 acagaggtca taccccgctt gtaaacggct gaaggggtgt gtttcttttt tgcgtgtact
     1501 tctcgagatc ttatc
//
